LOCUS       DAZKJR010000001        89206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKJR010000001 DAZKJR010000000
VERSION     DAZKJR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.001"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..1718
                     /gene="garD"
                     /locus_tag="ACHOWS_000001"
     CDS             147..1718
                     /gene="garD"
                     /locus_tag="ACHOWS_000001"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFQ0045657.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAIP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1867..2202
                     /gene="prlF"
                     /locus_tag="ACHOWS_000002"
     CDS             1867..2202
                     /gene="prlF"
                     /locus_tag="ACHOWS_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFQ0045658.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2202..2666
                     /gene="yhaV"
                     /locus_tag="ACHOWS_000003"
     CDS             2202..2666
                     /gene="yhaV"
                     /locus_tag="ACHOWS_000003"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFQ0045659.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2721..3530)
                     /gene="agaR"
                     /locus_tag="ACHOWS_000004"
     CDS             complement(2721..3530)
                     /gene="agaR"
                     /locus_tag="ACHOWS_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFQ0045660.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3779..5059
                     /gene="kbaZ"
                     /locus_tag="ACHOWS_000005"
     CDS             3779..5059
                     /gene="kbaZ"
                     /locus_tag="ACHOWS_000005"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFQ0045661.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCL
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5082..5555
                     /gene="agaV"
                     /locus_tag="ACHOWS_000006"
     CDS             5082..5555
                     /gene="agaV"
                     /locus_tag="ACHOWS_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFQ0045662.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5566..6345
                     /gene="agaW"
                     /locus_tag="ACHOWS_000007"
     CDS             5566..6345
                     /gene="agaW"
                     /locus_tag="ACHOWS_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFQ0045663.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6335..7213
                     /gene="agaE"
                     /locus_tag="ACHOWS_000008"
     CDS             6335..7213
                     /gene="agaE"
                     /locus_tag="ACHOWS_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFQ0045664.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPAQKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7231..7665
                     /gene="agaF"
                     /locus_tag="ACHOWS_000009"
     CDS             7231..7665
                     /gene="agaF"
                     /locus_tag="ACHOWS_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFQ0045665.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKLGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7662..8795
                     /gene="nagA"
                     /locus_tag="ACHOWS_000010"
     CDS             7662..8795
                     /gene="nagA"
                     /locus_tag="ACHOWS_000010"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0045666.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAEIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQILGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHAQQIWIQGQLASF"
     gene            9145..10299
                     /gene="agaS"
                     /locus_tag="ACHOWS_000011"
     CDS             9145..10299
                     /gene="agaS"
                     /locus_tag="ACHOWS_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFQ0045667.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10312..11172
                     /gene="kbaY"
                     /locus_tag="ACHOWS_000012"
     CDS             10312..11172
                     /gene="kbaY"
                     /locus_tag="ACHOWS_000012"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFQ0045668.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11339..11815
                     /gene="agaB"
                     /locus_tag="ACHOWS_000013"
     CDS             11339..11815
                     /gene="agaB"
                     /locus_tag="ACHOWS_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFQ0045669.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMSITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11854..12657
                     /gene="agaC"
                     /locus_tag="ACHOWS_000014"
     CDS             11854..12657
                     /gene="agaC"
                     /locus_tag="ACHOWS_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFQ0045670.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12647..13438
                     /gene="agaD"
                     /locus_tag="ACHOWS_000015"
     CDS             12647..13438
                     /gene="agaD"
                     /locus_tag="ACHOWS_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFQ0045671.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPLLKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHIGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13439..14206
                     /gene="agaI"
                     /locus_tag="ACHOWS_000016"
     CDS             13439..14206
                     /gene="agaI"
                     /locus_tag="ACHOWS_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFQ0045672.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(14256..15119)
                     /gene="rsmI"
                     /locus_tag="ACHOWS_000017"
     CDS             complement(14256..15119)
                     /gene="rsmI"
                     /locus_tag="ACHOWS_000017"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0045673.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            15184..17220
                     /gene="lpoA"
                     /locus_tag="ACHOWS_000018"
     CDS             15184..17220
                     /gene="lpoA"
                     /locus_tag="ACHOWS_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFQ0045674.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            17178..17573
                     /gene="yraN"
                     /locus_tag="ACHOWS_000019"
     CDS             17178..17573
                     /gene="yraN"
                     /locus_tag="ACHOWS_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFQ0045675.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            17593..18183
                     /gene="diaA"
                     /locus_tag="ACHOWS_000020"
     CDS             17593..18183
                     /gene="diaA"
                     /locus_tag="ACHOWS_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFQ0045676.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            18193..18768
                     /gene="dolP"
                     /locus_tag="ACHOWS_000021"
     CDS             18193..18768
                     /gene="dolP"
                     /locus_tag="ACHOWS_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFQ0045677.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(18881..19921)
                     /gene="yraQ"
                     /locus_tag="ACHOWS_000022"
     CDS             complement(18881..19921)
                     /gene="yraQ"
                     /locus_tag="ACHOWS_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0045678.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(19994..20629)
                     /gene="yraR"
                     /locus_tag="ACHOWS_000023"
     CDS             complement(19994..20629)
                     /gene="yraR"
                     /locus_tag="ACHOWS_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFQ0045679.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            20757..21275
                     /gene="yhbO"
                     /locus_tag="ACHOWS_000024"
     CDS             20757..21275
                     /gene="yhbO"
                     /locus_tag="ACHOWS_000024"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFQ0045680.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(21255..21698)
                     /gene="yhbP"
                     /locus_tag="ACHOWS_000025"
     CDS             complement(21255..21698)
                     /gene="yhbP"
                     /locus_tag="ACHOWS_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFQ0045681.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            21749..22051
                     /gene="yhbQ"
                     /locus_tag="ACHOWS_000026"
     CDS             21749..22051
                     /gene="yhbQ"
                     /locus_tag="ACHOWS_000026"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFQ0045682.1"
                     /translation="MTPWFLYLIRTADDKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(22038..22541)
                     /gene="yhbS"
                     /locus_tag="ACHOWS_000027"
     CDS             complement(22038..22541)
                     /gene="yhbS"
                     /locus_tag="ACHOWS_000027"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0045683.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(22535..23059)
                     /gene="ubiT"
                     /locus_tag="ACHOWS_000028"
     CDS             complement(22535..23059)
                     /gene="ubiT"
                     /locus_tag="ACHOWS_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFQ0045684.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            23268..24263
                     /gene="ubiU"
                     /locus_tag="ACHOWS_000029"
     CDS             23268..24263
                     /gene="ubiU"
                     /locus_tag="ACHOWS_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0045685.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            24272..25150
                     /gene="ubiV"
                     /locus_tag="ACHOWS_000030"
     CDS             24272..25150
                     /gene="ubiV"
                     /locus_tag="ACHOWS_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0045686.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            25231..26238
                     /gene="yhbW"
                     /locus_tag="ACHOWS_000031"
     CDS             25231..26238
                     /gene="yhbW"
                     /locus_tag="ACHOWS_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFQ0045687.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(26356..27600)
                     /gene="mtr"
                     /locus_tag="ACHOWS_000032"
     CDS             complement(26356..27600)
                     /gene="mtr"
                     /locus_tag="ACHOWS_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFQ0045688.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(27754..29643)
                     /gene="deaD"
                     /locus_tag="ACHOWS_000033"
     CDS             complement(27754..29643)
                     /gene="deaD"
                     /locus_tag="ACHOWS_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFQ0045689.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(29636..29716)
                     /gene="yrbN"
                     /locus_tag="ACHOWS_000034"
     CDS             complement(29636..29716)
                     /gene="yrbN"
                     /locus_tag="ACHOWS_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFQ0045690.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(29823..30707)
                     /gene="nlpI"
                     /locus_tag="ACHOWS_000035"
     CDS             complement(29823..30707)
                     /gene="nlpI"
                     /locus_tag="ACHOWS_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFQ0045691.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(30816..32951)
                     /gene="pnp"
                     /locus_tag="ACHOWS_000036"
     CDS             complement(30816..32951)
                     /gene="pnp"
                     /locus_tag="ACHOWS_000036"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0045692.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(33198..33467)
                     /gene="rpsO"
                     /locus_tag="ACHOWS_000037"
     CDS             complement(33198..33467)
                     /gene="rpsO"
                     /locus_tag="ACHOWS_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0045693.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(33616..34560)
                     /gene="truB"
                     /locus_tag="ACHOWS_000038"
     CDS             complement(33616..34560)
                     /gene="truB"
                     /locus_tag="ACHOWS_000038"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0045694.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(34560..34961)
                     /gene="rbfA"
                     /locus_tag="ACHOWS_000039"
     CDS             complement(34560..34961)
                     /gene="rbfA"
                     /locus_tag="ACHOWS_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0045695.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(35125..37797)
                     /gene="infB"
                     /locus_tag="ACHOWS_000040"
     CDS             complement(35125..37797)
                     /gene="infB"
                     /locus_tag="ACHOWS_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0045696.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(37822..39309)
                     /gene="nusA"
                     /locus_tag="ACHOWS_000041"
     CDS             complement(37822..39309)
                     /gene="nusA"
                     /locus_tag="ACHOWS_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0045697.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(39337..39789)
                     /gene="rimP"
                     /locus_tag="ACHOWS_000042"
     CDS             complement(39337..39789)
                     /gene="rimP"
                     /locus_tag="ACHOWS_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0045698.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(39996..40072)
                     /locus_tag="ACHOWS_000043"
     tRNA            complement(39996..40072)
                     /locus_tag="ACHOWS_000043"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40036..40038),aa:Met,seq:cat)
     gene            40420..41763
                     /gene="argG"
                     /locus_tag="ACHOWS_000044"
     CDS             40420..41763
                     /gene="argG"
                     /locus_tag="ACHOWS_000044"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFQ0045699.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(41771..43396)
                     /gene="yhbX"
                     /locus_tag="ACHOWS_000045"
     CDS             complement(41771..43396)
                     /gene="yhbX"
                     /locus_tag="ACHOWS_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFQ0045700.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(43853..43939)
                     /locus_tag="ACHOWS_000046"
     tRNA            complement(43853..43939)
                     /locus_tag="ACHOWS_000046"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43903..43905),aa:Leu,seq:gag)
     gene            complement(43954..44286)
                     /gene="secG"
                     /locus_tag="ACHOWS_000047"
     CDS             complement(43954..44286)
                     /gene="secG"
                     /locus_tag="ACHOWS_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0045701.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQIQPAAPA
                     KPTSDIPN"
     gene            complement(44514..45851)
                     /gene="glmM"
                     /locus_tag="ACHOWS_000048"
     CDS             complement(44514..45851)
                     /gene="glmM"
                     /locus_tag="ACHOWS_000048"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0045702.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(45844..46692)
                     /gene="folP"
                     /locus_tag="ACHOWS_000049"
     CDS             complement(45844..46692)
                     /gene="folP"
                     /locus_tag="ACHOWS_000049"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFQ0045703.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYHDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(46782..48716)
                     /gene="ftsH"
                     /locus_tag="ACHOWS_000050"
     CDS             complement(46782..48716)
                     /gene="ftsH"
                     /locus_tag="ACHOWS_000050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0045704.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(48816..49445)
                     /gene="rlmE"
                     /locus_tag="ACHOWS_000051"
     CDS             complement(48816..49445)
                     /gene="rlmE"
                     /locus_tag="ACHOWS_000051"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFQ0045705.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            49571..49864
                     /gene="yhbY"
                     /locus_tag="ACHOWS_000052"
     CDS             49571..49864
                     /gene="yhbY"
                     /locus_tag="ACHOWS_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0045706.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(50020..50496)
                     /gene="greA"
                     /locus_tag="ACHOWS_000053"
     CDS             complement(50020..50496)
                     /gene="greA"
                     /locus_tag="ACHOWS_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0045707.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            50744..52177
                     /gene="dacB"
                     /locus_tag="ACHOWS_000054"
     CDS             50744..52177
                     /gene="dacB"
                     /locus_tag="ACHOWS_000054"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFQ0045708.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(52218..53390)
                     /gene="cgtA"
                     /locus_tag="ACHOWS_000055"
     CDS             complement(52218..53390)
                     /gene="cgtA"
                     /locus_tag="ACHOWS_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFQ0045709.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(53406..54371)
                     /gene="yhbE"
                     /locus_tag="ACHOWS_000056"
     CDS             complement(53406..54371)
                     /gene="yhbE"
                     /locus_tag="ACHOWS_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFQ0045710.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLGPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(54498..54755)
                     /gene="rpmA"
                     /locus_tag="ACHOWS_000057"
     CDS             complement(54498..54755)
                     /gene="rpmA"
                     /locus_tag="ACHOWS_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0045711.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(54776..55087)
                     /gene="rplU"
                     /locus_tag="ACHOWS_000058"
     CDS             complement(54776..55087)
                     /gene="rplU"
                     /locus_tag="ACHOWS_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0045712.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            55346..56317
                     /gene="ispB"
                     /locus_tag="ACHOWS_000059"
     CDS             55346..56317
                     /gene="ispB"
                     /locus_tag="ACHOWS_000059"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFQ0045713.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            56545..56823
                     /gene="sfsB"
                     /locus_tag="ACHOWS_000060"
     CDS             56545..56823
                     /gene="sfsB"
                     /locus_tag="ACHOWS_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFQ0045714.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(56871..58130)
                     /gene="murA"
                     /locus_tag="ACHOWS_000061"
     CDS             complement(56871..58130)
                     /gene="murA"
                     /locus_tag="ACHOWS_000061"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0045715.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(58185..58439)
                     /gene="ibaG"
                     /locus_tag="ACHOWS_000062"
     CDS             complement(58185..58439)
                     /gene="ibaG"
                     /locus_tag="ACHOWS_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFQ0045716.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(58599..58892)
                     /gene="mlaB"
                     /locus_tag="ACHOWS_000063"
     CDS             complement(58599..58892)
                     /gene="mlaB"
                     /locus_tag="ACHOWS_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFQ0045717.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(58892..59527)
                     /gene="mlaC"
                     /locus_tag="ACHOWS_000064"
     CDS             complement(58892..59527)
                     /gene="mlaC"
                     /locus_tag="ACHOWS_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFQ0045718.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(59546..60097)
                     /gene="mlaD"
                     /locus_tag="ACHOWS_000065"
     CDS             complement(59546..60097)
                     /gene="mlaD"
                     /locus_tag="ACHOWS_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFQ0045719.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(60102..60884)
                     /gene="mlaE"
                     /locus_tag="ACHOWS_000066"
     CDS             complement(60102..60884)
                     /gene="mlaE"
                     /locus_tag="ACHOWS_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFQ0045720.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(60892..61701)
                     /gene="mlaF"
                     /locus_tag="ACHOWS_000067"
     CDS             complement(60892..61701)
                     /gene="mlaF"
                     /locus_tag="ACHOWS_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFQ0045721.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            61911..62888
                     /gene="yrbG"
                     /locus_tag="ACHOWS_000068"
     CDS             61911..62888
                     /gene="yrbG"
                     /locus_tag="ACHOWS_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFQ0045722.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            62902..63888
                     /gene="kdsD"
                     /locus_tag="ACHOWS_000069"
     CDS             62902..63888
                     /gene="kdsD"
                     /locus_tag="ACHOWS_000069"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFQ0045723.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGVTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            63909..64475
                     /gene="kdsC"
                     /locus_tag="ACHOWS_000070"
     CDS             63909..64475
                     /gene="kdsC"
                     /locus_tag="ACHOWS_000070"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFQ0045724.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSIAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            64472..65047
                     /gene="lptC"
                     /locus_tag="ACHOWS_000071"
     CDS             64472..65047
                     /gene="lptC"
                     /locus_tag="ACHOWS_000071"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFQ0045725.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            65016..65573
                     /gene="lptA"
                     /locus_tag="ACHOWS_000072"
     CDS             65016..65573
                     /gene="lptA"
                     /locus_tag="ACHOWS_000072"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFQ0045726.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            65580..66305
                     /gene="lptB"
                     /locus_tag="ACHOWS_000073"
     CDS             65580..66305
                     /gene="lptB"
                     /locus_tag="ACHOWS_000073"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFQ0045727.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            66353..67786
                     /gene="rpoN"
                     /locus_tag="ACHOWS_000074"
     CDS             66353..67786
                     /gene="rpoN"
                     /locus_tag="ACHOWS_000074"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0045728.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            67809..68096
                     /gene="hpf"
                     /locus_tag="ACHOWS_000075"
     CDS             67809..68096
                     /gene="hpf"
                     /locus_tag="ACHOWS_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFQ0045729.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            68214..68705
                     /gene="ptsN"
                     /locus_tag="ACHOWS_000076"
     CDS             68214..68705
                     /gene="ptsN"
                     /locus_tag="ACHOWS_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFQ0045730.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            68751..69605
                     /gene="rapZ"
                     /locus_tag="ACHOWS_000077"
     CDS             68751..69605
                     /gene="rapZ"
                     /locus_tag="ACHOWS_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0045731.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            69602..69874
                     /gene="npr"
                     /locus_tag="ACHOWS_000078"
     CDS             69602..69874
                     /gene="npr"
                     /locus_tag="ACHOWS_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFQ0045732.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            70087..70719
                     /gene="yrbL"
                     /locus_tag="ACHOWS_000079"
     CDS             70087..70719
                     /gene="yrbL"
                     /locus_tag="ACHOWS_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFQ0045733.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(70716..71444)
                     /gene="mtgA"
                     /locus_tag="ACHOWS_000080"
     CDS             complement(70716..71444)
                     /gene="mtgA"
                     /locus_tag="ACHOWS_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFQ0045734.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(71441..72094)
                     /gene="elbB"
                     /locus_tag="ACHOWS_000081"
     CDS             complement(71441..72094)
                     /gene="elbB"
                     /locus_tag="ACHOWS_000081"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFQ0045735.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(72324..74660)
                     /gene="arcB"
                     /locus_tag="ACHOWS_000082"
     CDS             complement(72324..74660)
                     /gene="arcB"
                     /locus_tag="ACHOWS_000082"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFQ0045736.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLALVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(74756..75685)
                     /gene="yhcC"
                     /locus_tag="ACHOWS_000083"
     CDS             complement(74756..75685)
                     /gene="yhcC"
                     /locus_tag="ACHOWS_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0045737.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQTECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            76360..80820
                     /gene="gltB"
                     /locus_tag="ACHOWS_000084"
     CDS             76360..80820
                     /gene="gltB"
                     /locus_tag="ACHOWS_000084"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFQ0045738.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            80833..82251
                     /gene="gltD"
                     /locus_tag="ACHOWS_000085"
     CDS             80833..82251
                     /gene="gltD"
                     /locus_tag="ACHOWS_000085"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFQ0045739.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            82434..83561
                     /gene="yhcG"
                     /locus_tag="ACHOWS_000086"
     CDS             82434..83561
                     /gene="yhcG"
                     /locus_tag="ACHOWS_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFQ0045740.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(83621..84085)
                     /gene="nanQ"
                     /locus_tag="ACHOWS_000087"
     CDS             complement(83621..84085)
                     /gene="nanQ"
                     /locus_tag="ACHOWS_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFQ0045741.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQCDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(84082..84957)
                     /gene="nanK"
                     /locus_tag="ACHOWS_000088"
     CDS             complement(84082..84957)
                     /gene="nanK"
                     /locus_tag="ACHOWS_000088"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFQ0045742.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALHDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHCSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(84954..85643)
                     /gene="nanE"
                     /locus_tag="ACHOWS_000089"
     CDS             complement(84954..85643)
                     /gene="nanE"
                     /locus_tag="ACHOWS_000089"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0045743.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(85691..87181)
                     /gene="nanT"
                     /locus_tag="ACHOWS_000090"
     CDS             complement(85691..87181)
                     /gene="nanT"
                     /locus_tag="ACHOWS_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFQ0045744.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(87291..88184)
                     /gene="nanA"
                     /locus_tag="ACHOWS_000091"
     CDS             complement(87291..88184)
                     /gene="nanA"
                     /locus_tag="ACHOWS_000091"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFQ0045745.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(88306..89097)
                     /gene="nanR"
                     /locus_tag="ACHOWS_000092"
     CDS             complement(88306..89097)
                     /gene="nanR"
                     /locus_tag="ACHOWS_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFQ0045746.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        21691 a        24045 c        22463 g        21007 t
ORIGIN      
        1 cggcactgca atccctgaaa tgattgacat tgatcacatt tctgcattta aactcctgac
       61 attcttattt cacccaatga agttatttat ttttaaatga gaccaggtcc tcattttaat
      121 aacccctggc tggagaatat tgcacaatgg ccaacatcga aatcagacaa gaaacgccaa
      181 ctgcgtttta tataaaagtt cacgacacag ataatgtggc aattattgtt aatgataatg
      241 gcctgaaagc aggaacgcgt tttccggatg ggctggaatt aattgaacat attccccagg
      301 ggcataaagt cgcattgctg gacattccgg ctaatggtga aattattcgt tatggcgaag
      361 tgattggtta cgccgtgcgc gcaatcccac gcggaagctg gatcgacgaa tcaatggttg
      421 tactaccgga agcgccgccg ttacacacgc tgccactggc aaccaaagtc ccggcaccct
      481 taccgccact ggaaggatac acctttgagg gctatcgcaa tgccgatggc agcgtgggca
      541 ccaaaaacct gctcggcatc accaccagcg tccactgtgt ggcaggcgtg gtggactatg
      601 tagtaaaaat cattgaacgc gatctgctgc cgaaataccc gaacgtcgat ggcgtggtgg
      661 ggctgaatca tctgtacggt tgtggcgtgg cgattaacgc tccggcggcg gtggtgccca
      721 ttcggaccat tcacaacatt tcgcttaacc caaactttgg cggcgaggtg atggtgattg
      781 gcctgggttg cgagaaatta cagccagaac gcctgctggt cggcacggat gatgtacaag
      841 gtattccgct ggagaacgcc agtattgtca gcttgcagga tgaaaagcat gtcggttttc
      901 agtccatggt cgaggatatt ttgcaggtcg ccgaacgcca tttgcacaaa ctgaatcaac
      961 ggcagcgaga aacctgtcca gcttcagaac tggttgtcgg catgcagtgc ggtggcagcg
     1021 atgcgttttc tggcgtaacg gcaaacccgg cggttgggta tgcgtctgat ctactggtgc
     1081 gctgcggcgc aacggtgatg ttttcagaag tcacggaagt gcgtgacgcg atccatctgc
     1141 tgacaccacg cgcagtgaac gaagaggtcg gcaaacggct gctggaggag atggagtggt
     1201 acgataacta tctcaatatg ggaaaaaccg accgtagcgc caacccttcg ccgggcaaca
     1261 agaaaggcgg tctggcaaat gtggtggaga aagcgctcgg ctccattgct aaatcgggta
     1321 aaagcgcaat tgttgaagtg ctgtcgcccg gtcaacgccc gactaaacgc ggattaattt
     1381 acgccgcgac gccagccagc gattttgtct gtggcacaca acaggtggct tcagggatta
     1441 ccgtgcaagt gtttacgacc ggtcgcggta cgccgtacgg cctgatggcg atacccgtca
     1501 ttaaaatggc gacccgcacc gagctggcga accgctggtt tgatttaatg gatattaacg
     1561 cgggcactat cgctaccggc gaagaaacca ttgaagaagt gggctggaag ttgttccact
     1621 ttattctcga cgtcgccagc gggaagaaga aaactttctc ggatcaatgg ggactgcata
     1681 accagctggc ggtgtttaac ccggcaccag tgacctgatt tcttttggaa cgtccctcgc
     1741 aaaacatggc cttagtgcca tgtttttatt gtttaaagtc cacacatcca ttaataatgc
     1801 atttgcatta ccttagttca agcttataat ttgagcagaa aacaggctgt aaaaggacag
     1861 taaataatgc ccgctaatgc tcgctctaac gctgtactga ccactgaatc aaaggtcact
     1921 atacgcggac aaacaactat ccccgcgcca gtgcgtgagg ccttaaaact gaagccaggc
     1981 ctggacagca ttcattacga aattctgcct ggtgggcaag tatttatgtg ccgactggga
     2041 gatgaacagg aggatcatac tatgaatgca tttttgcgtt ttctggatgc agatatccag
     2101 aacaacccgc aaaaaactcg tccattcgac attcaacaag gaaagaaact tgtcgctggc
     2161 atggacgtca acattgacga tgagattggc gacgacgaat aatggatttt ccacaaaggg
     2221 ttaatggttg ggcgctatat gctcatccct gttttcagga aacctacgat gctttagttg
     2281 ccgaagtcga ggcattaaag ggaaaagatc ctgaaaatta tcagagaaaa gctgccacaa
     2341 agttattggc ggtggtccat aaagtgattg aggagcatat cacggtcaat ccatcatcac
     2401 cggcattccg tcatggcaag tcgttaggct ctgggaaaaa taaagactgg tcacgggtaa
     2461 aatttggtgc tggtcgctat cgtctcttct ttcgttacag tgaaaaagag aaagtcatca
     2521 ttctgggatg gatgaacgat gaaaacacgc tgcgcaccta cggtaaaaaa acagatgcct
     2581 ataccgtatt cagtaaaatg ttaaaaagag gacatcctcc tgccgactgg gaaagcctca
     2641 cccaagagac agaagaaaac cattgatggt gttcacaatg cccgccgaac gcattggtgt
     2701 tgaccagcgg gcatcctttc tcattccccg accagaatca cttcaacccc agcctttctc
     2761 agtccttcca gactatccgc aggaatgcct tcatcaacaa tgatcatgtc gatacgttga
     2821 gtatcaatga tcttatgtaa actagaacga ttgaacttac tggaatcggt gaccacgatg
     2881 atccgttccg caacttcgca catccgacgg tttaaacggg cttcatcttc attatgcgtg
     2941 ctgacgccgc gctccagatc gatcgcatct acaccaagaa acagcatatc gaagtggtaa
     3001 ttttgcagcg attgctcagc ctgatcaccg taaaaagatt gcgactgacg gcgcaaatgc
     3061 cccccggtca tcagcagctc aacgccttcc gcttccagca acgcattagc cacgttcata
     3121 ccgttggtca tcgcaattac gtcggtgtgc ttgcgcatca gacgagcaat ctcaaaagtg
     3181 gtggtcccgg agtcgaggat cacccgatga ccaggctgaa tcaactcaac ggcagctttc
     3241 gcaacgctgc gtttcatcgc ggtgttcagc gcgcttttat cttccactga tggctcgact
     3301 gacggcgttg tgctatcgca gatcaacgcg ccaccatagg cacgcacagc gatcccctgc
     3361 ttttccagaa acgccagatc gttgcggatc gtcacagtag atacaccata caacgccgat
     3421 agatcattaa cttgcacact tccttgctgg cgcaggcgtt gaatgatctg ctctcgtcgc
     3481 tcgctggtgc ctgtcactcg cttctcacct gaagcgtcgg tattactcat agtaagtcct
     3541 ttcgtaaaac tttcgtttca tttcgttttg cctattaacg cctttctatt aagcaaatgc
     3601 aagcccacct tacccattgg cgcaagctac tctcgtttca gtggctttca ttatgtttct
     3661 tttgtgaatc agatcagaaa accattatct ttcgttttat ttttatctca ccatgacgca
     3721 gtatcaactg aaacaaaacg aaagattaat gtcgcagtaa tctgaactgg agaggaaagt
     3781 gaaacatctg acagaaatgg tgagacagca caaagcgggc aaaacaaatg ggatttatgc
     3841 cgtttgttcc gcacatccgc tggtgctgga agctgcaatc cgctacgcca gtgcaaacca
     3901 aacgccgtta ctgattgaag caacctccaa tcaggtggac cagttcggcg gttataccgg
     3961 aatgacgccc gccgattttc gcggctttgt ttgtcagctc gccgactcgt tgaatttccc
     4021 gcaggatgcg ctgattctgg gtggtgacca tctggggcca aaccgctggc aaaacctgcc
     4081 tgccgctcag gcaatggcca atgccgatga tttgattaaa agctacgtgg cggcaggatt
     4141 caaaaaaatt caccttgatt gcagcatgtc ctgtcaggac gatccgattc ccttaaccga
     4201 tggcatcgtg gccgaacgcg ccgcccgtct ggcgaaagtg gcggaagaaa cctgtcttga
     4261 acactttggc gaagccgatc tggtgtatgt cattggcacc gaagtaccgg tacctggcgg
     4321 cgcgcatgaa accttaagcg agctggcggt caccacgccg gatgccgccc gcgccacgct
     4381 ggaagcccat cgtcacgcct ttgaaaagca aggtctgagt gccatctggc cacgcatcat
     4441 tgctctggtg gtacaacccg gcgtcgaatt cgatcacacc aacgttattg attatcagcc
     4501 cgtcaaagcg accgccttaa gccagatggt cgaaaactac gaaacgctga ttttcgaagc
     4561 gcactctacc gattaccaaa cgccgcaatc gttgcgccag ctagtgattg accactttgc
     4621 catcctgaaa gttggcccgg cgctgacctt cgccctgcgt gaagctctgt tctctctggc
     4681 ggcgattgaa gaagaactgg taccagcaaa agcctgttct ggtctgcgtc aggtgctgga
     4741 aaacgtgatg ctcgaccgcc cggaatactg gcaaagccac taccacggtg acggcaacgc
     4801 gcgtcgtctg gcgcgtggtt atagctactc ggatcgcgtg cgctattact ggccggacag
     4861 ccagattgat gacgctttcg ctcatctggt tcgtaatctg gcggattcac caattccgct
     4921 gccgctgatc agtcagtatc tgccgctgca gtacgtgaaa gttcgctccg gcgagctgca
     4981 gccaacgcca cgggaactca ttatcaacca tattcaggac atcctggcgc agtaccacac
     5041 agcctgtgaa ggccaataag caaaacaaag aggaacacgc tatgccaaat attgttttaa
     5101 gtcggattga tgaacgcttg attcacggtc aggtcggcgt tcagtgggtc ggatttgcgg
     5161 gggcaaatct ggtgctggta gccaacgatg aggttgccga agatccggta caacaaaacc
     5221 tgatggaaat ggtactggca gaagggatcg ccgtacgttt ctggacgctg caaaaagtca
     5281 tcgacaacat tcatcgcgcc gccgatcgac agaaaatcct gctagtttgt aaaacaccgg
     5341 ccgatttcct gacgttggtg aaaggtggcg ttccggtgaa tcgcattaac gttggcaata
     5401 tgcactacgc caatggcaag caacaaatcg ccaaaacggt ttctgtggat gcgggtgata
     5461 tcgcagcatt taacgatctg aaagccgctg gggtggaatg cttcgttcag ggcgtcccga
     5521 cagagcctgc tgtagacctc tttaaattac tttgagggat tcatcatgga aatcagtctg
     5581 ttgcaggcat ttgcgttggg aattatcgcc tttatcgctg gcctggatat gtttaacggc
     5641 ttaacccata tgcaccgccc ggtggttctc ggcccgttgg tcgggctggt acttggcgat
     5701 ctgcataccg gtattttaac cggcggtacg ctggaactgg tgtggatggg gctggcccct
     5761 ctggcgggcg cacagccgcc taacgtgatt atcggtacta tcgtcggcac ggcgtttgcc
     5821 attactactg gcgtgaaacc cgatgtcgcg gtcggtgtcg ccgtaccttt cgctgtcgca
     5881 gtacagatgg ggattacctt cctgttctcg gtgatgtccg gcgtgatgtc ccgatgcgac
     5941 cggatggcgg agaacgccga tactcgcggc attgaacgcg tgaactatct ggcattgctg
     6001 gcactcggca cgttctattt tctctgcgct ttcctgccta tctacttcgg cgcggaacat
     6061 gcaaaaacca tcattgatgt cctgccgcaa cgattaattg atggcctcgg cgtcgcgggc
     6121 ggcatcatgc cagcaatcgg ttttgccgtg ctgctgaaaa tcatgatgaa aaacgtctac
     6181 atcccctact tcatcctggg ctttgttgcc gcggcctggc tcaagttacc ggtgctggct
     6241 attgctgccg ctgcgctggc gatggcgctg atcgacctgc tgcgtaaatc tcctgaaccg
     6301 actcaacctg cggcacagaa agaggaattt gaagatggca tctaatcaaa ccaccctgcc
     6361 gaacgtcagc gaaaatgaag aaacactgct gactggcgtc aatgaaaacg tgtatgaaga
     6421 tcagagcatt ggcgcggagc tgacgaaaaa agatatcaac cgcgtcgcct ggcgttccat
     6481 gctgttacag gcttctttca actacgaacg tatgcaggct tccggctggc tgtacggcct
     6541 gctgccagca cagaaaaaga tccacactaa taaacgcgac ctggcgcgcg ccatgaaggg
     6601 gcatatgggt ttcttcaata cccatccgtt tctggtgacg tttgttatcg gcattatcct
     6661 tgcgatggag cgttctaagc aggacgttaa cagtattcag agcaccaaaa ttgccgtcgg
     6721 tgcgccgctc ggcgggattg gcgatgcgat gttctggcta acgctactgc cgatttgtgg
     6781 cgggataggt gcaagcctcg cgctacaagg ctctattctt ggcgctgtcg tctttattgt
     6841 gctgttcaac gtggtgcacc tggggctgcg ttttggtctg gcgcattatg cttaccgcat
     6901 gggcgtggcg gcgattccac tcattaaagc aaataccaaa aaagtcggcc atgcggcatc
     6961 tatcgttggg atgacggtaa tcggcgcgct ggtggcaacc tatgttcgtt taagcaccac
     7021 gctggaaatc accgcgggcg acgcagtggt taagttacag gctgatgtta tcgacaaact
     7081 gatgccagcc ttcttaccgc tggtctacac cctgaccatg ttctggctgg tacgccgcgg
     7141 ctggagtccg ctgcgcctga ttgcagtgac cgtggttctc ggcatcgtcg gtaaattctg
     7201 ccatttcctt taaatacaat gaggtattag atgttaagta ttattttgac agggcatggc
     7261 gggtttgcca gtggcatgga aaaagcaatg aagcaaatcc tcggcgagca atcgcagttt
     7321 attgccatcg attttccgga aacctcaagc accgcgctgc ttacatcgca gcttgaagag
     7381 gccatcgccc aactggattg tgaagacggc attgtttttc tgacggattt gctgggcggc
     7441 acgccgtttc gcgtagcttc aacgctggcg atgcaaaaac tgggctgtga agtgattacc
     7501 ggcaccaatt tgcaactgct actggagatg gtgctggagc gcgaagggtt aagcggtgaa
     7561 gagtttcgtg tgcaggcgct ggagtgtgga catcgtgggc tgaccagtct ggtggacgag
     7621 ttaggccgct gccatgagga atgtccggtc gaggaaggaa tatgacaccc gttttgcgcg
     7681 ccagaaggct gctgactgaa gagggatggc tcgatgacca tcagttgcgt attgctgacg
     7741 gtgtaatcgc agaaatcgaa ccgattccag tgggcgtgac tgaacgcgat gcggaactgc
     7801 tctgccccgc ttacatcgac acccatgtac acggtggtgc gggcgttgat gtaatggatg
     7861 acgcgccgga tgtgctcgac aagctggcaa tgcacaaagc acgcgaaggt gtcggcagtt
     7921 ggttaccgac caccgtaacc gcgccgctta ataccattca tgcggcgctg gaacgtattg
     7981 ctcaacgttg ccaacgcggc ggacctggtg cacaaatact ggggagttat ctcgaaggac
     8041 cgtacttcac gccgcagaat aaaggcgcgc atccgccgga gctgtttcgc gagctggaaa
     8101 ttgccgagct ggatcaattg attgccgttt ctcagcacac cttgcgcgtg gtggcgctgg
     8161 caccggaaaa agaaggggca ttgcaggcca tccgccatct taaacagcaa aacgtaaggg
     8221 tgatgctggg gcatagcgcg gcgacctggc aacaaacccg cgccgcgttt gatgctggtg
     8281 ccgacggcct ggtgcattgc tataacggga tgacaggttt acatcaccgc gaaccgggaa
     8341 tggttggcgc gggattaacg gacaagcgcg cctggctgga actgatagcc gatggtcatc
     8401 atgtgcatcc ggcggcgatg tcgctgtgtt gttgctgtgc gaaagagaga atcgtgctca
     8461 tcaccgacgc gatgcaggca gccgggatgc cggatggtcg ctatacgtta tgtggcgaag
     8521 aagtgcagat gcacggtggc gttgtccgta ccgcgtccgg tggactggcg ggcagtacgc
     8581 tgtctgtcga tgcggcagtg cgcaacatgg tcgagttgac gggcgtaacg cctgcggaag
     8641 ccatccatat ggcgtcgctg catccggcgc gtatgctggg tgttgatgat gttctgggat
     8701 cgcttaaacc gggcaaacgc gccagcgtcg ttgcgctgga tagcgggctg catgcgcaac
     8761 aaatctggat tcagggtcaa ttagcttcgt tttgatggtt tgctccttta ttgggccttc
     8821 acttcccccg taaggccttt ctttttcttt cgttttgatc tgtgcagcgg tgtcggatgc
     8881 gacgctaatg cgccttatcc gacctacggt tggtgaccgt aaggccggat aaagtattta
     8941 cgccgcatcc agcaatccct tttacttccc ttgtcttttc tttcaacgat cacagatttc
     9001 gttttatttc ctttttctcc attgaacttt cagtttcttt tctatagatt ttaaccaacg
     9061 aaagaaatca ccaagtgaaa tgaaacgaaa ggcaaacgaa agcgacaacg ccgacgtcaa
     9121 gttcatcaga ctaaggattg agttatgcca gaaaattaca cccctgctgc tgccgcaacc
     9181 ggtacatgga ctgaagaaga gatccgccat cagcctcgcg catggatccg ttcactcacc
     9241 aacatcgacg cgctacgttc cgcgctcaat aacttccttg aaccgttact gcgcaaagag
     9301 aatctgcaaa tcatcctgac cggagccgga acatcggcat ttatcggtga catcatcgcg
     9361 ccgtggctcg ccagtcatac cggtaaaaac ttcagcgccg taccgaccac cgatctggtc
     9421 actaatccga tggactacct gaatccagct catccgctgc tgttgatctc cttcggtcga
     9481 tccggcaaca gcccggaaag cgtcgccgcc gtggaactgg caaatcaatt tgtacccgag
     9541 tgctatcacc tgccgatcac ctgcaacgaa gcgggcgctc tttaccaaaa cgcgatcaac
     9601 agcgacaacg cgtttgccct actgatgccc gcagaaacgc acgaccgcgg ctttgcgatg
     9661 accagcagca ttaccaccat gatggccagt tgcctcgcgg ttttcgcacc tgagacgatc
     9721 aacagccaga gcttccgcga cgtggcggat cgttgccagg cgatcctgac ctcactgggc
     9781 gatttcagcg aaggtgtgtt tggttacgca ccgtggaaac ggatcgttta tctcggcagc
     9841 ggtggcttac agggcgcagc acgcgagtcg gcgctgaaag tgctggaact gacagcgggt
     9901 aaactggcgg ccttctatga ttccccgacc ggattccgtc atggcccgaa atcgctggtc
     9961 gataacgaaa cgctggtggt ggtatttgtc tccagccacc cttacacccg tcagtatgat
    10021 cttgatctgc tggcagaact ccgccgcgat aaccaggcaa tgcgcgtaat cgccatcgcc
    10081 gcggaaagca atgacgttat tactgccggt ccacatatca tcctgccgcc gtcccgtcac
    10141 tttatcgacg ttgagcaggc attttgcttc ctgatgtacg cccagacatt tgcactgatg
    10201 cagtcgctgc acatgggtaa tacaccggat accccatcag ccagtggcac cgttaaccgc
    10261 gtggtgcaag gcgtaatcat tcatccgtgg caggcataag aggatcgcat tatgagcatt
    10321 atctccacca aatatctgtt acaggacgcc caggccaatg gctacgcggt gcctgccttt
    10381 aacattcaca acgccgagac gatccaggcg atcctcgaag tgtgcagtga aatgcgatca
    10441 ccggtgatcc tcgccgggac gccggggacc tttaagcata tcgcgctgga agagatctac
    10501 gccctgtgta gcgcctattc cacaacctac aacatgccgc tggcactgca tctcgaccac
    10561 cacgaatcgc tggatgatat tcgccgtaaa gtccacgcag gcgtgcgcag cgcgatgatc
    10621 gacggcagtc acttcccgtt tgccgagaac gtgaagctgg tgaaatcggt tgtcgacttc
    10681 tgccactccc aggattgcag cgtggaagca gaactgggcc gcctcggcgg tgttgaagat
    10741 gacatgagcg ttgacgccga aagtgcattc ctgaccgatc cacaagaagc taaacgcttt
    10801 gtcgaactga ctggcgtcga cagcctggcg gtagcgattg gtacggcgca cggcttatac
    10861 agcaaaacgc cgaagattga tttccagcgg ctggcggaaa ttcgtgaagt ggtggatgtt
    10921 cctctggtgc tgcatggtgc cagcgatgtc ccggatgaat ttgtccgtcg cactattgaa
    10981 cttggcgtca caaaagtgaa cgttgccaca gaattaaaaa tagccttcgc tggcgcggtt
    11041 aaagcctggt ttgcggaaaa tccacagggt aatgatcctc gttattatat gcgcgtcggt
    11101 atggatgcga tgaaagaagt tgtcagaaat aaaattaatg tctgtggttc agcgaatcga
    11161 atttcagcat aatcattagt tttttatgat ttatcccaat gtacttcccg attaatatcg
    11221 gggagtgcct taatggtaaa ggagataact aaaccttaat aaatacatca gtaaaatatc
    11281 gcaacaataa tatatttaaa aaaattatat tattcaactt tatggtgagg atcacacaat
    11341 gagcagtcca aatattctct taacccgtat tgataaccgt ctggttcacg gtcaggttgg
    11401 cgtgacctgg acatccacca tcggtgcgaa tctgctggta gtcgtggatg atgttgtcgc
    11461 taacgatgat attcaacaga aattaatgag tattaccgca gaaacctacg gctttggcat
    11521 tcgtttcttt actatcgaaa aaaccattaa cgtcatcggc aaagctgcac cgcatcaaaa
    11581 gatcttcctg atttgccgta cgccacaaac ggtacgtaaa ttggtagaag gtggtattga
    11641 cctgaaagat gtcaacgtcg gcaatatgca tttctcggaa gggaaaaagc aaatcagcag
    11701 taaagtttat gtcgatgacc aggatctcac ggacttacgt tttattaaac aacgtggcgt
    11761 gaatgttttc attcaggacg tccctggcga tcaaaaagaa caaatccctg actaaatcta
    11821 aaatcgcctt aatattggtt tgaggtaata aaaatgcatg aaataaccct acttcaggga
    11881 ttatccctgg cggcgttagt tttcgttctg gggattgatt tttggctgga agccttattt
    11941 ttattccgcc cgataatcgt ttgtacccta actggcgcta ttctcggtga tattcagact
    12001 ggcttaatta ccggtggtct gacagagttg gctttcgccg gattaacccc tgcaggtggt
    12061 gttcagccgc ccaacccgat tatggcgggt ctgatgacca ccgtcattgc atggtctacg
    12121 ggcgttgatg ccaaaacagc aattggtctt ggcctgccgt ttagtttgtt aatgcagtat
    12181 gtcattctgt tcttctattc tgctttctca ttatttatga ccaaagccga taaatgcgcg
    12241 aaagaagcgg atacggcagc gttttcccga cttaactgga caacgatgct catcgttgct
    12301 tcagcgtatg cggtgattgc tttcctctgt acttacctgg cacaaggggc gatgcaggcg
    12361 ctggtgaaag cgatgcccgc ctggctgacc cacggctttg aagtggctgg cggtattctg
    12421 cctgccgttg gttttggctt gctgctgcgc gtgatgttca aagcgcaata tatcccttac
    12481 ctgatcgccg gcttcctgtt tgtttgctac atccaggtca gcaacctgtt gccggttgcc
    12541 gtactgggcg caggctttgc ggtgtatgag tttttcaatg cgaaatcccg gcagcaagcg
    12601 caaccgcagc ccgttgccag taaaaatgaa gaagaggact acagcaatgg gatctgaaat
    12661 cagtaaaaaa gatatcaccc gtctgggctt tcgttcgtca ctgctacaag caagctttaa
    12721 ctacgaaagg atgcaggcgg gcggctttac ctgggcgatg ttgccgctcc tgaaaaagat
    12781 ttataaggac gacaaaccag gcctaagcgc ggcgatgaaa gataacctag aatttattaa
    12841 tacccacccg aatctggtcg gattcctgat ggggttatta atttcgatgg aagagaaagg
    12901 ggaaaaccgc gacaccatta aaggcctcaa agtggcgctg tttggcccaa tcgccgggat
    12961 tggcgatgcg attttctggt ttaccttgtt accaattatg gcggggattt gctcatcgtt
    13021 tgccagtcag ggaaacctgc tggggccgat tctgttcttt gccgtttacc tgcttatctt
    13081 tttcttgcgc gtcggctgga ctcacatcgg ttactcggtc ggcgtgaagg ctattgataa
    13141 agtgcgagag aactcgcaga tgattgcccg ttcggcaacc atcctcggga tcacggtaat
    13201 cggcgggctg atcgcttcgt atgtgcatat taacgtggtg acatcgtttg ccatcgacag
    13261 tacccacagc gtcgcgctgc agcaggattt cttcgataaa gtcttcccga acattttacc
    13321 gatggcctac accctgctga tgtattactt cctgcgggtg aaaaaagcgc atccggtgct
    13381 gttaatcggc gtgacgtttg ttctttctat tgtttgttct gcattcggca ttttgtaaat
    13441 ggaacgaggc accgcgtctg gcggtgcctc tttacttaag gaatttcatc ctgtgcaaac
    13501 ccttcaacaa gttgaaaact atacggcgtt aagtgaacgt gccagcgaat atttattggc
    13561 cgtgatccgt agcaaaccgg atgccgtgat ttgcctggcg accggagcca cgccattact
    13621 gacgtatcat tatctggtag aaaaaatcca ccagcagcag gttgatgtca gccagctcac
    13681 cttcgtgaag ctcgacgaat gggtggatct gccattaacg atgcctggta cctgcgaaac
    13741 tttcctgcaa cagcatatcg tgcagccgct ggggctacgt gaagaccagc tcatcagctt
    13801 tcgctccgaa gagattaatg agacagagtg cgaacgggta acgaacctga ttgcgcgcaa
    13861 aggcggtctg gatttatgcg ttctcgggtt ggggaaaaac ggtcatcttg ggctgaacga
    13921 accgggagaa agcctgcaac cggcctgcca tatcagtcaa cttgatgcca gaacacagca
    13981 acatgagatg ttaaaaaccg cgggccgtcc tgtgactcgt gggatcacct taggcctgaa
    14041 ggatattctc aatgcccgcg aagttttgtt actggtgact ggcgaaggaa agcaggatgc
    14101 gacagaacgt tttctcacgg ctaaagtctc taccgctatc ccggcttcat ttttatggct
    14161 gcataactat ttcacatgca tgattgatga gatgtgtcgt cgttaactcg cctgctacag
    14221 gcgttgcaac gctattcctg ctgcgacgcc tgtaatcact ctccctgctg ctccagcgca
    14281 tacttataca gcgcattttt cttcacgccg tgaatctctg ccgccagcgc cgccgctttt
    14341 ttcagcggca gttctgcctg tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct
    14401 tcttcctgcg ctttatggcc ttcgacaatc agcaccattt cgcctttgcg acggttttca
    14461 tcttctttga cccacgccag cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt
    14521 ttggtcagct cacgcgccag aaccacgtag cgggattcgc ctaataccgc aacgatatct
    14581 tccaggctat ctaacagacg gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct
    14641 tcaatagctt ttagcgcatc acggcggcct tttgatttgg caggtaaaaa gccttcataa
    14701 caaaaacgat cggatggtaa acccgcggcg cttaacgcag tgatagcagc acacggccct
    14761 ggtagcggca ccacgcgaat ccccgcttca cgacaggtac gcaccagatg gtagccagga
    14821 tcgttaatta gcggcgttcc ggcatcggaa accagcgcaa tgttttgccc ctcttgcagt
    14881 ttcgccagca gcgtttcggc tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg
    14941 gcattaatcc caaaatgttg cagcaataaa ccggtgtgac gcgtatcctc ggcggcaatc
    15001 agatcaacgg cctgtaatac ctctaatgca cgctgggtga tatcagccag attgccgatt
    15061 ggcgtcggta caatgtaaag ctggccctga gaattatccg ccgattggtg ttgtttcatt
    15121 gtgtcgtccg tattgccgat ttaatattga gcattgcgta aaaaaaatat cactggatac
    15181 attatggtac cctcaacatt ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg
    15241 gcagccctga ttttcgccgg ttgtggcacc catactcccg atcagtccac tgcttatatg
    15301 cagggcacgg cgcaggctga ttctgccttt tacctgcaac agatgcagca aagttctgat
    15361 gataccagga tcaactggca attactcgcc attcgtgcac tggtgaaaga aggtaaaacc
    15421 ggacaggcgg tggagttgtt taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc
    15481 gagaaaacac tgctggcggc agagattaaa ctggcgcaga aagattttgc tggcgcgcaa
    15541 aacttgctgg cgaaaatcac acctgccgat ttagaacaaa accagcaagc ccgttactgg
    15601 caggcaaaaa tcgatgccag ccaggggcgt ccttccattg atttactgcg cgcgttaatt
    15661 gctcaggaac cgctgctcgg tgcgaaagaa aagcagcaga atattgacgc cacatggcag
    15721 gcgctctcct ccatgactca ggagcaggcg aatacgctgg tgatcaacgc cgacgaaaat
    15781 attctgcaag gctggctgga tttgcagcgc gtctggtttg ataaccgtaa cgatcctgac
    15841 atgatgaaag ctgggatcgc cgactggcag aaacgttatc cgaacaaccc gggcgcgaaa
    15901 atgctaccaa cgcagttggt taacgtaaaa gcgtttaaac ccgcatcgac caacaaaatc
    15961 gccctgctgt tgccgctgaa tggccaggca gcggtatttg gtcgcactat tcagcaaggc
    16021 tttgaagcgg cgaaaaatat cggcactcag ccagtggcgg cccaggtagc agccgcacct
    16081 gccgcagacg tagcagaaca acctcagccg caaaccgtgg atggcgttgc cagcccggca
    16141 caagcttcgg ttagcgagct gaccggtgaa cagcctgcgg cccagccggt acctgtaagc
    16201 gccccggcga caagcaccgc agcggtaagc gcacccgcaa atccatccgc agagctgaaa
    16261 atctacgata cctcatcaca accacttagc cagatcttaa gccaggttca gcaggatggt
    16321 gcgagtattg tggtcggtcc gttgctgaaa aataacgttg aagagttgct gaagagcaac
    16381 accccgctga acgtgctggc actgaaccag ccggagaaca tcgaaaaccg cgtcaatatt
    16441 tgttacttcg cgctttcacc ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac
    16501 cagggtaaac aagcgccgct ggtgctgatc ccacgcagtt cattgggcga ccgcgtagcc
    16561 aatgcgtttg cgcaagagtg gcagaaactg ggcggcggca ccgttctgca acaaaaattt
    16621 ggttccacca gcgaattacg cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt
    16681 agcccgatta ctcccagagc gacaaccgac tccggcatga cgaccaacaa tccaacgctg
    16741 caaaccacgc caaccgatga ccagttcacc aataatggcg gtcgtgtcga tgcggtgtac
    16801 attgtggcaa cgccgggtga aatcgctttt atcaaaccga tgatcgccat gcgtaacggt
    16861 agccagagcg gtgcaacgct gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg
    16921 gatttccgtc tggagatgga aggcttgcag tatagcgaaa tcccgatgct ggcaggcggt
    16981 aatctgccgt taatgcagca ggcgctcagc gcggtgaaca acgattattc gctggctcgc
    17041 atgtatgcga tgggcgtcga tgcctggtcg ttggcaaatc atttctcaca aatgcgccag
    17101 gttcagggtt ttgaaatcaa cggtaatacc ggaagtctga cggctaaccc ggattgcgtg
    17161 attaacagga agttatcatg gctacagtac caacaaggtc aggtagtccc cgccagttaa
    17221 ccaccaaaca gaccggcgat gtgtgggaag cacaagcgcg tcgctggctg gaaggcaaag
    17281 gactgcggtt tatcgccgct aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc
    17341 gtgaaggccg gatcaccgtt tttgtcgagg tgcgttaccg ccgctctgcg ctttatggcg
    17401 gcgcggcagc cagtgtgacc cgcagcaaac aacacaaatt attacagact gcccgcttgt
    17461 ggctcgcgcg tcataatggg agttttgata ctgtggattg ccggttcgat gtggtagcct
    17521 tcaccgggaa tgaggttgag tggattaagg atgcctttaa tgaccactca taattaaggt
    17581 ttaaggatta gcgtgcaaga aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa
    17641 attgcggcgg cagaggcgct tccggatgcc atctcgcgtg cagccatgac gctggttcag
    17701 tctctgctca atggcaacaa aatcctctgt tgtggtaatg gaacttccgc tgccaatgca
    17761 cagcattttg ctgccagcat gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc
    17821 attgcactaa atactgataa tgttgtctta acggcgattg ccaacgatcg cttacatgat
    17881 gaagtgtatg caaaacaggt gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt
    17941 tccacccgtg gcaacagccg cgatattgta aaagcggtag aagctgccgt tacgcgtgat
    18001 atgaccattg tggcattaac cggctatgac ggcggtgaac ttgcaggttt gttagggcca
    18061 caggatgtgg agatccgcat tccttcgcat cgtagtgctc gcattcagga aatgcatatg
    18121 ctgacggtaa attgcctgtg cgatctgatc gataacacgc ttttccctca ccaggatgtt
    18181 taaggagaat acatgaaggc attatcgcca atcgcagtcc ttctttccgc gctgctgttg
    18241 caaggttgtg ttgccgctgc cgtagtgggt accgctgctg taggtaccaa agccgcaact
    18301 gacccacgca gtgtcggcac ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc
    18361 gcattgtcga aagacgaaca aattaagaaa gaagcgcgca ttaatgtcac ggcctatcag
    18421 ggcaaagtgc tgctggttgg gcagtcacca aacgctgaac tttcggctcg cgccaaacag
    18481 attgctatgg gcgtagacgg tgccaacgaa gtgtataacg agattcgtca gggccagccg
    18541 attggtctgg gcgaagcgtc taacgatacg tggatcacca ccaaagtacg ttcgcagctg
    18601 ttaaccagcg acctggtgaa atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg
    18661 ttcctgatgg ggctggtgac tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg
    18721 gtaagcggcg tgaagcgggt aactacggcg tttacgttta ttaaatagca gcccttgtaa
    18781 tgcctgatgc gacgcttgca gcgtcttatc aggcctacaa cgatgcaaac cgtaggtcgg
    18841 ataaggcgtt tacgccgcat ccgacatgtt ttccctcaaa tcaaaccaac agcgccagcc
    18901 cgccgacaat cacaccagac actgccacca tcgcccccgt cagccataag gctttcgccg
    18961 ggaacgcttt gcgcagcata atcagtgacg gcaaactcac cgccgggagc gtcatcaaca
    19021 atgccagcgc cggagcagtt cccatacctg ccagcatcat cgtttgtaca atcggaattt
    19081 ctgctgccgt gggaatgaca aacaagcatc ccgctaccgc catcgccacc acccacatca
    19141 ggctgttatc gacagcacca tcggcatggg ggaataacca gacgcgagcg gcacccaaca
    19201 ccagtactgc aaggatgtaa accgggatcg tactccagaa aagcgtccat agcgccctgc
    19261 cccagcggct aaaaaatcct ccctgtgctt ccggtatgtc aatttcgacc ggtgcctgcg
    19321 tttgcggtgt ttcacgcacc catttttgca ccagcgtcgc aatcagcaac accatcacca
    19381 gccccgccac cagacgaatc gccgcaaaac cccagccgag gacgaacccc ataaacacca
    19441 gcgtcgccgg gtttaacaac ggattcccca tccagaatgc cagcgcaccg cccatcgaca
    19501 cctgttggcg acgcattccc gccgcgaccg gagccgcaca gcaggtacac atcatgcccg
    19561 gcagcgaaaa cagcgttccc agcagcgtgc cgcgaaagcg cgattgccca agcgtacgca
    19621 ataaccagtc acgcgggatc agcacctgaa tcaacgaccc gagcagtacg cccagcaccg
    19681 ccgctttcca taccgcgagg aaatagatca tcgcgtaatc caacgccgcc tgccacggat
    19741 tagcatccgc ctgcgcaagg atagatttac cgatactgtg ggtttcggca gcagtaaacg
    19801 ctttgccgta gtaaggttgc catttcacat accagagacc agcaatgaca acgagaaaga
    19861 aaagcgcggg tttccaccac tgaatgggcg ttgccgcctg agatgaagac tgaccagtca
    19921 tagcattccc caggaataag ttatgataat tgagcgcgtg aatattacgc tcactatcaa
    19981 ttcttgggaa taattattca gctcttttgc gtaattctga agagcttaaa atcgtcacac
    20041 cttcatgctc aggccgcatc gcctctgcca gcatgacccg cgccacgtct ctcgcatcaa
    20101 tggatttcca gttacccggt aacaaacgga acagcggcgc aaaaagcgtt tcgttcatcc
    20161 gctgtttgct acgatcgccc agtaacatcg acgggcgagc aatggtcaat ttcggccagt
    20221 tctgggcgat taatgcttct tccatctccc ctttgacgcg gttatagaaa aacggcgagt
    20281 gggcattagc acccatagca ctgaccacca gcatatgctg cgcaccgagc cgccgcccgg
    20341 ttaatgcggt atccaccacc agcgtgtaat cggcatgaat aaacgcctct ttactccccg
    20401 cttctcgccg cgtggtgccg agacaacaaa acacaatgtc gatgggatcg gtgacctgcg
    20461 ccagcgcgtc ggtcagttgc ggatcatggg gattaaacac accgggcata tcgcccaacg
    20521 gacgtcgcgt cggcgcggct atggcgttaa ctttcggttc attaatcaac atccgcagca
    20581 ggtgaccacc caccagcccc gttgcgcctg taatcagtac ctggctcatc ttcgctcctt
    20641 tacagaattg tctgccttgc gctccccggc tcatgcacca ggcttaatag tctacgaggt
    20701 aattatcccc gattgtggga aattcgcctc atccaatgca acaacgcgga ggaagcatga
    20761 gtaagaaaat tgccgtttta atcactgatg aatttgagga ttcagaattt acttcacccg
    20821 cagacgagtt ccgtaaagcc ggacacgaag tgattaccat tgaaaaacaa gcgggtaaaa
    20881 cggtgaaagg caaaaaagga gaagccagcg tggccatcga taaatccatc gatgaagtga
    20941 cgccagcgga gtttgatgcc ctgctgctac cgggcggcca ttcaccggat tatctgcgtg
    21001 gcgacaaccg ttttgtcact ttcacccgtg attttgtgaa tagcggcaaa cctgttttcg
    21061 ccatctgtca cggtccgcag ctgctgatca gcgccgatgt gattcggggg cgcaaactga
    21121 ctgccgttaa accgatcatt attgatgtta aaaacgcggg cgcggaattt tacgatcagg
    21181 aagtggtggt cgataaagat cagctggtta ccagccgtac accggacgat ctgccagcgt
    21241 ttaaccgcga ggcattacgc ctgctcggtg cctgagttac gtagccagat cattttttta
    21301 ccaaagccca gcgtgttgtc ggtgaatttg atttcatcga gccggatttc ccacaccggg
    21361 gccgacagca ttctggcaac cggaaagcgg cgattgtacg cctggcgcgc aaggtcgctt
    21421 tcctcacctt caagcctgcg gatctcgcct ttaaattgca cgccgcgaat taacgctacc
    21481 gttttcggct gaccgtttac cgttccggca actgccgcct gcggcccact catctgcgcg
    21541 tggcgcgttt tttcttccgt caaaatgtag aaggcgactt tctgcgcatc aaaaagataa
    21601 aaggcattag cgcaccagag ttccccttcc tgctgcacac accaggtgac aacatgttgt
    21661 tttgccagcc aacggctgat ggcggtgagt gtttccattg ctgttctctt ttatactgtg
    21721 ggcctgacct taacatgcga cgcagacgat gacaccctgg tttctttact tgatccgtac
    21781 cgccgacgat aagctttata ccgggatcac cacagatgtc gaacgccgct atcagcagca
    21841 ccaaagcggc aaaggggcga aagcactgcg cgggaaagga gaactaacgc tggcgttttc
    21901 cgcgccagtg ggcgatcgtt cgctggcact acgggcggaa tatcgcgtta agcaactgac
    21961 caaacggcaa aaagagcgtc tggtagcgga aggcgcaggg tttgcagaac tgttgagcag
    22021 cctgcaaacc ccaaagatta aaagcgattg aagtgctcgt gatactcaac cagaccagtt
    22081 acaccgttca gcgcgtcatc cgctaaacga tgtacctgga aggcactttc agtgcccggc
    22141 cagcggcaac gcagatcgtg atgtgccgcc agttcaaagc cgaaacggct gtacagcgcc
    22201 ggatcgccca gcgtcaccac tgcggcatag ccgaactcat taagcgaatc gagtccttca
    22261 tagaccagtt ggcgtgccag cccctgtcca cggtattttt catctaccgc cagcggtgcc
    22321 atgccgaccc attgcaggtc ttcgccctgc acatcaaccg gactaaatgc cacatagcca
    22381 atgacctgac cttcgtcatc tgtcgccacc agccccagcg tcagaaagcc atcttcacgc
    22441 agatcgtgaa ccagcttcgc ttccgcatca ctttcgaatg aacgacgcag cagggcatca
    22501 atacccggcg catcaatggg aatttctact cgaattagca tggttcacct accgatgtct
    22561 gtttggtttc aggcgcggtt ttcattcccg cctcaacaaa atccgccagt tgcagcagca
    22621 tcatgcgtaa ggctttcggc atttgctcca gttcaatggc gtccatcagg tttttcacat
    22681 acagccccag ctccgtatcg ccttcaatta ccagccgacg ctggaagaag agcgtatccg
    22741 gatcttgttt acgcgccgca atcatcagca gatcgctggc gtcggcacta aaactcacat
    22801 cagcttgcgc gttctggcta acgaccagtt tgccattcac caccgaggta aaccattgca
    22861 ggtcaatatc acgcacatga atacttaacc agcggccttc aagaaactcc agctcgccat
    22921 catccaacgc ctggcggaat tgccagctta agacctgctc aagaacctgg cgttttagcg
    22981 caaatggcgt cagttttacc ggtacactca acagagatgg ccccaaatgc acaatacggg
    23041 aacgcagttt atccaacacg agttttactc cctgtttcaa caatcatcct attttgccat
    23101 atcagaaaaa taacatagcg gtataaatca acaattccat atgaaattgc tgctaccacc
    23161 aatacaactt taactgcctt aaatcaaaaa ttgtcgcagc aaggttaact aaaatcccag
    23221 ttcgttaaca tttttgcgct ttgatagcgc aaccttcagg aaaaattatg gagctgctct
    23281 gccctgccgg aaatctcccg gcgcttaagg cggccatcga aaacggcgca gatgctgttt
    23341 atatcgggct aaaagatgat accaatgccc gtcacttcgc cggccttaac tttaccgaga
    23401 aaaaattgca ggaagcggtg agttttgtcc atcaacatcg ccgcaaactt cacatcgcga
    23461 ttaacacttt tgcgcatccg gacggttacg cccgctggca gcgcgccgtg gatatggcgg
    23521 cgcagttggg tgccgacgcg ctgatcctcg ccgacctcgc catgctggag tacgccgccg
    23581 agcgttaccc gcatattgag cgtcatgtgt cagtgcaggc ttcggcgacc aatgaagagg
    23641 cgattaactt ttatcatcgc cattttgacg ttgcccgcgt ggtgctgccg cgcgtgttgt
    23701 cgattcatca ggtgaaacaa ctggcacggg tcacacctgt cccgctggaa gtgtttgctt
    23761 tcggcagcct gtgcattatg tcggaaggcc gttgctatct gtcgtcgtat ctgacgggtg
    23821 agtcgcccaa caccgtgggc gcgtgttctc cggcccgttt cgtgcgctgg cagcaaacgc
    23881 cgcagggact ggaatctcgc ctgaacgaag tgctgatcga ccgttatcag gatggcgaaa
    23941 acgcaggtta tccgacgctg tgtaaagggc gttatctggt ggacggcgag cgctatcacg
    24001 cgctggaaga acccaccagt ctcaataccc tggaactgct gccggagtta atggcggcga
    24061 atattgcttc ggtgaaaatt gaaggccgcc agcgtagccc ggcgtatgtc agccaggtgg
    24121 cgaaagtctg gcgtcaggct atcgaccgtt gtaaggccga tccgcaaaac ttcgtaccgc
    24181 aaagcgcgtg gatggagacg ctcgggtcga tgtccgaagg cacgcaaact acccttggcg
    24241 cgtatcaccg taaatggcag tgagaaaagc aatgaaatat tccttagggc cggtgctatg
    24301 gtactggcca aaagagacgc tggaagactt ttatcagcag gccgccgcca gcagcgcaga
    24361 cgtgatttat cttggtgaag cggtctgtag caaacgccgg gcaaccaaag ttggcgactg
    24421 gctggagatg gcaaaatcgc tcgccggaag tggcaagcag attgtgctct ccacgctggc
    24481 gctggtgcag gcgtcctccg aacttggcga actgaaacgt tatgttgaga acggtgagtt
    24541 tctgattgaa gccagcgatc tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt
    24601 cgtcgccggg cacgcgctga actgctacaa cgcggtgaca ctgaaaatat tgctcaaaca
    24661 gggcatgatg cgctggtgta tgccggtgga gctttcccgc gactggctgg tgaatctgct
    24721 taatcagtgc gatgagctgg gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg
    24781 tcatctgccg ctggcctact ccgcccgctg ctttaccgcg cgttcggaag accgcccgaa
    24841 agatgagtgt gaaacctgct gcattaagta tccgaacggg cgcaacgtgc tatcgcagga
    24901 aaaccaacaa gtgtttgtac tcaatggcat tcagaccatg agcggctacg tttacaacct
    24961 cggtaacgag ctggcatcca tgcaggggct ggtcgatgtg gtccgcctgt caccgcaggg
    25021 tactgacact ttcgcgatac tcgacgcctt ccgcgccaat gaaaatggcg ctgcaccact
    25081 gccgttgacg gcaaacagcg attgtaacgg ctactggcgg cggctggccg ggctggagct
    25141 gcaagcctaa gtaaatagct cactttgtta acaactttaa ctactcttta atgcagtatt
    25201 aaagattaat cagtaacaaa gtgagctgtt atgactgata aaaccattgc gttttcgcta
    25261 ctcgatctgg cccccattcc cgaaggctct tcagcgcggg aagcgttctc ccactctctc
    25321 gacctcgccc gtctggctga aaagcgcggc tatcatcgct actggctggc ggaacaccac
    25381 aatatgaccg gcattgccag tgctgcaaca tcagttttga ttggctatct ggcggcgaat
    25441 accactacgc tgcatctggg gtctggcggc gtgatgttgc ctaaccactc accattggtg
    25501 atcgccgaac agttcggcac gcttaatacg ctctatccgg ggcgaatcga tttggggctg
    25561 ggtcgtgcgc cgggtagtga ccagcgaacc atgatggcac tacgtcgtca tatgagcggt
    25621 gatattgata atttcccccg cgatgtcgcg gagctggtgg actggtttga cgcccgcgat
    25681 cccaatccgc atgtgcgccc ggtaccaggc tatggcgaga aaatccccgt gtggttgtta
    25741 ggctccagcc tttacagcgc gcaactggcg gcgcagcttg gtctgccgtt tgcgtttgcc
    25801 tcacacttcg cgccggatat gttgttccag gcgctgcatc tttatcgcag caacttcaaa
    25861 ccgtcggcac gactggaaaa accatacgcg atggtgtgca tcaatattat cgctgccgac
    25921 agcaaccgcg acgctgaatt tctgtttacc tcaatgcagc aagcctttgt gaaactacgc
    25981 cgtggcgaaa ccggacaact accgccaccg attcaaaata tggatcagtt ctggtcaccg
    26041 tctgagcagt atggcgtgca gcaggcgctg agtatgtcgt tggtaggtga taaagcgaaa
    26101 gtgcgtcatg gcttgcagtc gatcctgcgc gaaaccgacg ccgatgagat tatggttaac
    26161 gggcagattt tcgaccacca ggcacgcctg cattcgtttg agctggcgat ggatgtgaag
    26221 gaagagttgt tggggtagtg tgttttaacg taggaagcct tatccgagat ggcaacgctg
    26281 tcctacgtag gcctgataag cgaagcgcat caggcattgt gtaggcagca gaaatgtcgg
    26341 ataaggcacc gctgattact gatacaccgg cagcaaatta aagctcgata agatatggac
    26401 cagtgcgttg ccaacgccaa ataccagaat cagcataatc atcggcttgc cgccccagac
    26461 gcggaatttc gggctaccaa agcgtttacg cgatgcgcgg gccaacagcg ccggaacaat
    26521 tgccgcccag atggtagccg ctaagccagc ataaccaatg gcgtacagga atccgttagg
    26581 aaacagcagg ccacccacaa caggcggggc aaaggtcagc aacgctgttt tcaaacggcc
    26641 catagccgag tcgtcgaaac caaacagatc tgccagatag tcaaacaaac ccagcgttac
    26701 gccaaggaac gaactcgcta ccgcaaagtt tgagaacacg accagcagaa gatccaggct
    26761 acggctgttc agcacgccgc ttaatgcctg taccagcaca tcaatattac cgcccttctc
    26821 ggcgatgccg ataaactccg gacgagggat gttgcccatc gtcgccagca accagatggt
    26881 atacagcgcc agcgccatca gcgtaccgta caccaggcat ttcacgatgg ttttcggatc
    26941 tttgccgtaa tacttcatca ggctcggcac gttaccgtga taaccaaacg atgccagaca
    27001 gaatggcagt gtcatcagca gatacggcgc ataagacgca ttgctttcgg cgacgttgaa
    27061 caaggtcgtt ggctgtacat gccccagcag gctgccgaag gtgaggaaga aagtaatgac
    27121 tttcgccccc agcacaattg ctgtcatccg gctgacagct ttcgtactca accacaccac
    27181 gaacgctacc agcagtgcaa aaccaaatcc agctgcccgt gcagggacgt tcagcgacat
    27241 ctccgcgaag gtgtgatgca gaatcgaacc gctggcagaa atataggcgt aagtcaggat
    27301 atagagcaca aaggcaatgg aaatgccgtt gactacgttc cagcctttgc ccagcaaatc
    27361 tttggtgatg gtgtcaaagc tagaaccgat tcgataattc aggttagctt ccagaatcat
    27421 caagccggaa tgcagcatac agaaccaggt aaagatcagc gccgccattg accagaagaa
    27481 ccacgccccg gacatgacca caggcagaga aaacatccct gcgccaataa tggtgccgcc
    27541 gataatcacc acgccgccaa gcagcgacgg tgacgtttgg gtggtggtta gtgttgccat
    27601 gagggcttct ctccagtgaa aaatagtgcg actgcgttgt tatgcattgc actgtaccag
    27661 tacacgagta caaaagacag aaaaaaagcc ccgatggtaa aaatcggggc tgtatatatt
    27721 attttacaga ttgtgttcgc tgttcagcga tgattacgca tcaccaccga aacgacgacg
    27781 accggtagag tcatcacgac gcggagcgcg gccttcacga cgttcgccgc taaaacgacg
    27841 accatcacca cgaccacctt cacggcgttc accgctgaag ttacgaccgc cttcacgacg
    27901 ttcgccactg aaaccacgac caccgccacg acgctcaccg ccagtatgcg gctgtgcatc
    27961 gcccagtaac tgcatgttca tcggcttgtt gagaatgcga gtgcgcgtaa agtgttgcag
    28021 cacttcaccc ggcatacctt tcggcagttc gatggtggag tgagaagcaa acagcttgat
    28081 gttaccaatg taacggctgc tgatgtcgcc ttcgttagca atcgcaccaa cgatatgacg
    28141 aacttcaaca ccatcatcgc ggcccacttc aatgcggtac agctgcatat cgccaacatc
    28201 acgacgttca cgacgcggac gatcttcacg gtcaccacgc gggccacggt cgttacgatc
    28261 gcgcggacca cggtcatcac ggtcacggaa ttcacgtttc ggacgcatcg gcgcatctgg
    28321 cggaacaatc agagtacgtt caccctgtgc cattttcagc agtgcggcgg ccagagtttc
    28381 gagatccagc tcttcacctt ctgccgtcgg ctgaattttg ctcagcagtg cacggtattg
    28441 atccagatcg ctgctttcca gctgctgctg tactttagcg gcgaattttt ccagacgacg
    28501 tttgcccagc agttctgcgt tcggcagttc aacttccgga atggtcagct tcatggtgcg
    28561 ttcaatgtta cgcagcagac gacgctcacg gttctcaacg aacagcagcg cacggccagc
    28621 acggcccgca cgaccagtac gaccgatacg gtgaacgtaa gactcagaat ccatcgggat
    28681 atcgtagtta actaccaggc tgatacgctc aacgtccagg ccacgggccg caacgtcggt
    28741 cgcaatcagg atgtccagac gaccatcttt cagtcgttcc agtgtctgct cacgcagcgc
    28801 ctggttcatg tcaccgttca gcgcggcgct gttgtagccg ttacgctcaa gggcttctgc
    28861 cacttccaga gtcgcgtttt tggtacgaac gaagataatc gccgcatcaa aatcttccgc
    28921 ttccaggaaa cgtaccagtg cttcgttttt acgcataccc cagacagtcc agtagctctg
    28981 gctgatgtca ggacgggtag tcacgctgga ctgaatgcgc acttcctgcg gctctttcat
    29041 aaagcggcgg gtaatgcgac gaatcgcttc cggcatggtt gcagagaaca gagcggtctg
    29101 atgaccttcc gggatctgcg ccataatggt ttcaacgtct tcgatgaagc ccatgcgcag
    29161 catttcgtca gcttcatcca gaaccagacc gctcagttta gagaggtcca gagtgccacg
    29221 tttcaggtgg tccagcagac ggcccggagt accgacaacg atctgcggcc cctgacgcag
    29281 ggcgcgtaat tgcacgtcat aacgttggcc gccgtacagg gcaaccacgt ttacgccgcg
    29341 catgtgttta gagaaatccg tcattgcttc agcaacctgt accgccagtt cgcgggtcgg
    29401 tgccagcacc agaatctgtg gtgctttcag ctcaggatca agattctgca gcagaggtaa
    29461 agagaatgct gcagtttttc cgctccccgt ctgggccata cccagaacgt cgcggccatt
    29521 cagcagatgt ggaatacact ctgcctgaat tggagatggt ttttcgtaac ccagatcgtt
    29581 aagggcttca aggataggag ccttcaggcc cagatctgca aaagtggttt cgaattcagc
    29641 catgtagtac gtgtgcctca aaattaatgg cggccagtct acataactca tcatgaaatt
    29701 gatcagcaat tttcattgaa aagtgtgaac cggctcaaag taggtgtatt aacgaacaac
    29761 aacgccctca cccgttaagg tgatggcaat caaaaaagat tacgggctga tgtgtacgtc
    29821 agctattgct ggtccgattc tgccaggtca tcttggtcct ggcccaggag cgataattcc
    29881 aacaatgcgt atcggtgctc aacaaagtta tgaacgttgt tggcaaccgc cagtttgaac
    29941 agtgccgtgg cgctgtccaa atcccccaga cttaggtagt acttacctaa atagaagttg
    30001 gtttcactga gatgctcagc gagcgaggtg ttatccgttg cgtccgcctt gagcctttcc
    30061 attaacgttt gttcgctaat gttgcccagg tagaactcga caatgttcca tccccactgt
    30121 tccttatccg atttttcgaa gtgctgtttc aacacttctt tagcctgctt ctcatcgagc
    30181 ttctgctcgg cgagataaag ccacagacta cggaaaggat cattgggatc gtcttgataa
    30241 aacgccagca gatcatcttg cgctaacttg tcacgaccgc cgtaatataa tgcgatcccg
    30301 cgattcaagt gcgcgtagtt gtaagttgga tcaagctcaa gtacagaatc aaacgcttca
    30361 taggcagcat caaaattgcc tgcctgcgtt aaatatatgc ctaagtaatt gaatacttca
    30421 ggcatatccg gtcggattgc cagcgcttgc gaaaaatcgt tacgcgctaa tgccctcaga
    30481 ccgagactat catacaacac tccgcgctca tataaaagct gtgcgcgttc gtcatcggtt
    30541 aaagcccgac tggcaaggat ttgttccata cgtgccagaa tcacttcctg ctgtaaagtc
    30601 ggttgcaatg gtaccgcgag gacttcactt ttacgccagg aagtattact gcatcctgca
    30661 agcgtaagtg ctgtcgcaac gaaacaccag cgcaaaaaag gcttcatttc ccactcccga
    30721 agaccacggt tgaatgaacg tcctgttccc ggttgctaac aaggcgtcct gcccggttaa
    30781 aagccccccg ccgcagcgga gggcaaatgg caaccttact cgccctgttc agcagccgga
    30841 gcttccggtg ctgcagcagg ttgagactgc tcagtcgctt ctttaatgct cagacggata
    30901 cggccctggc gatcaacttc cagaactttc accggtactt cctgacccat ctgcaggtaa
    30961 tcggtcactt tctcaacgcg tttgtcagcg atctgagaga tgtgtaccag accttcttta
    31021 ccgccgccga tggcaacaaa tgcgccaaag tcaacgatac gggtcacttt accattgtag
    31081 acgcggccca cttcgatttc tgcagtgatc tcttcgatac gacgaatagc atgtttcgct
    31141 ttctcgccgt cggtcgctgc gattttcaca gtaccgtcat cttcgatttc gatggtggtg
    31201 ccagtttctt cggtcagagc acggataaca gaaccgcctt taccgataac gtctttgatc
    31261 ttgtccgggt tgatcttgat ggtatggata cgcggagcga actcagagat atcgccgcgc
    31321 ggcgcgttga tcgcctgttc cattacgccc aggatatgca gacgcgcacc tttagcctgg
    31381 ttcagcgcaa cctgcatgat ctctttggtg ataccttcaa ttttgatatc catctgcaat
    31441 gcagagatac cgtcacggga acccgcaact ttgaagtcca tatcgcccag gtgatcttcg
    31501 tcgcccaaaa tgtcagacag tacaacgtag ttgtcgcctt ctttcaccag acccattgcg
    31561 atacccgcaa cggcagcttt gatcggcaca cctgcgtcca tcagcgccag agacgcgccg
    31621 cacacggaag ccatagaaga ggaaccgttg gattcggtga tttcagacac aacacgtacg
    31681 gtatacggga atttgtccat atccggcatg actgccagca cgccgcgctt cgccagacga
    31741 ccgtgaccaa tttcacgacg cttcggagaa ccgaccatgc cggtttcgcc tacagagtac
    31801 ggagggaagt tgtagtggaa caggaaggtg tcagtacgtt cgcccatcag ttcatcaaga
    31861 acctgcgcgt cacgtgcagt acctagcgtt gcggtaacca gcgcctgagt ttcaccacgg
    31921 gtgaacagcg cagaaccgtg agtacgcggc agcacaccag tacgcacatc cagaccacgg
    31981 atcatatctt tttcacgacc gtcgatacgc ggttcgcctg ccagtacgcg gctacgaaca
    32041 acatttttct cgatagcgtg cagaatttca cccagttcgt tttcgtccag ggtttcgtct
    32101 tcagcaagca gtgtcgcgat ggtttcagat ttgatgacat caacctgcgc ataacgctct
    32161 tgtttgtcgg tgatgcggta agcatcgctc agacgagctt cagccagtgc agcaacgcgc
    32221 gcgttcagcg cttcgtttac cggctccggc tgccagtccc aacgcggttt accggcttct
    32281 ttcaccagtt cattgatgtt ctgaataaca acctgctgtt gttcatgacc gaagactact
    32341 gcgcccagca tctggtcttc gctcagcagt tcagcttcag attcaaccat cagtacagcg
    32401 gcttcagtac ccgcaacaac cagatccagt ttgctctctt tcaactcgtc ctgagtcggg
    32461 ttcagtacgt actggtcatt gatgtaacct acacgggcag caccaatcgg gccattgaac
    32521 ggaataccag acagagacag ggctgcggaa gcaccaatca tcgcgacgat atccgggtta
    32581 acctgcgggt taacagaaac cacggtggcg ataacctgaa cttcgttgac gaagccttcc
    32641 gggaacagcg ggcgaatcgg gcggtcaatc agacgcgcga tcagggtttc gccttcgctt
    32701 gggcggcctt cacgacggaa gaagctaccc gggatacgac cagcagcgta ggtacgctcc
    32761 tgatagttaa cggtcagcgg gaagaagtcc tgacctggtt tggctttttt ctgaccaaca
    32821 acggtaacga ataccgcagt gtcatccatg ctaaccataa cagcggcagt agcctgacga
    32881 gccatcatgc cggtttccag agtcacggtg tgttggccgt actggaattt acgaacgatc
    32941 ggattaagca atgtaatatc ctttctcttt cttagacagt accttacggc actggtgtta
    33001 atacccgatc ttctgcgcat cctcgcgact aatgacaacc ctaacccagc tctatgtggg
    33061 taaagcctct cattagccgc gcgaacctct gcagcggaag atcattcata gcaacaatac
    33121 attagtttcc agtgaattgc tgccgtcagc ttgaaaaaag gggccactca ggcccccttt
    33181 tctgaaactc gcaagaatta gcgacgcaga cccaggcgct cgatgagctg ggtgtaacgt
    33241 gctacgtctt tacgtttcag gtagtcgagc agtttacgac gctgagaaac catgcgcagc
    33301 agaccacgac ggctgtggtg atcttttttg tgctctgcaa agtggccctg caggtggttg
    33361 atctgtgcag tcagcagtgc tacctgaact tcggtagaac cggtgtcgtt tgcgtcacga
    33421 ccaaactcag aaacgatttt agctgttgct tcagtactta gagacatttt aaaactccaa
    33481 agtatataga atgaaaggac gccgatctct aattcagcga tcccagtgta cgttacgcaa
    33541 agtgttaaac aatttacgcg acgttaagcg gcagtattct actcgtagcg acttgttatc
    33601 gcaagacggt taacattacg ccgggtattc aaccaccagg cgacgaggcg caacgcggcc
    33661 ttcatcgtca atttcgccca taccgataaa cttgccgttc tcaccctctg taacgcgaac
    33721 cagtccttcc agcggcgcac cggatgtacg aactgggtta ccatttttga agtaaacaga
    33781 agacgttaac ggaagattca ccactggata gtccgaagct ggactgtcca ttggcatcag
    33841 taatggatca agtaactccg cagccggaat atcctgctgt tcggcttgct caaccagttc
    33901 acgcagatgt tccagggtca ccatccgttc aaccggatat ttacttaccg ccagacgacg
    33961 caagtaaata acatgcgcgc cacaaccgag tttttcgccg agatcgtcaa tgatagtgcg
    34021 gatataagtg ccttttgagc agtgaatttc cagctccagc tcattgcctt catggcgaat
    34081 aaacagcaat tcataaacgg taatcgggcg cgcttcacgc ggaacttcaa tgccctgacg
    34141 cgcgtattcg tacagttttt tgccctgata tttcagtgct gaatacatcg aagggatctg
    34201 ttcgatatcg ccacggaagg tatccagcgc cgccgccagt tgctctgcac taaaggttac
    34261 cggacgttct tcaacgattt gtccgtcggc atcagaagta tcggtacgct gtccaagacg
    34321 cgcaatgacc cgataacgtt tgtcggagtc cagcagatac tgggaaaact tcgtcgcttc
    34381 cccgaggcaa attggcaaca tgccggtcgc cagcgggtcc agcgcaccgg tatgcccggc
    34441 acggttggcg ttataaatcc gtttcacttt ttgcagcgca tcgttgctgg acatcccctg
    34501 cggtttatcc agcaacaaaa cgccgttaat gtcgcgaccg cgacgacgag gacgactcat
    34561 tagtcctcct tgctgtcgtc cgggttaaca cgacgttctt cgtcatgttt gaccacgctg
    34621 gtcaccaggt ttgacatgcg cattccttca accagagagt tgtcgtagaa gaaggtcagt
    34681 tccggcacga tacgcaggcg catcgctttc cccagcaggc tgcggatgaa accagaagct
    34741 tcctgcagcg ctttgatgcc cgctttaacc gcgtcttcat ctttgtcgtt gaggaacgtt
    34801 acatatactt tggcatacgc caggtcgcga gacatttcga caccggaaac ggtggtcatc
    34861 atgcccagac gaggatcttt aatttcacgc tgcaggatga gagcgatctc tttttgcatt
    34921 tcctgcgcta cgcgctgcgg gcgaccaaat tctttcgcca taataaattc tcctgacaaa
    34981 aaaggggctg ttagcccctt tttaaaatta atttcaggtg gaagggctgt tcacgttggc
    35041 ctgataagac gcgccagcgt cgcatcaggc aatccatgcc ggatgcggcg taaacgcctt
    35101 atcccgcatg gaaccctaaa aaccttaagc gatggtacgt tggatttcga tgatttcgaa
    35161 tacttcgatc acatcgccag tgcggacgtc gttgtagttc ttaacgccga taccacattc
    35221 cataccgtta cggacttcgt taacgtcatc tttgaagcgg cgcagggact ccagctcgcc
    35281 ttcgtagata accacgttgt cgcgcagaac gcggatcggg ttgtgacgtt taaccacacc
    35341 ttcggtaacc atacaacctg cgatggcacc aaatttcggt gatttgaata cgtcacgaac
    35401 ttccgccaga ccgataatct gttgtttcag ttccggagac agcataccgc tcattgccgc
    35461 tttcacttcg tcgatcaggt tatagatgac ggagtagtaa cgcagatcca ggctttccgc
    35521 ttcaatcact ttacgtgcag aggcatcagc acgtacgttg aagccaacca ggatggcatt
    35581 ggacgccgca gccagggtag cgtcggtttc ggtgatacca cctacgccag aaccgatgat
    35641 cttcacttta acttcgtcag tagacagttt cagcaaggag tcggagatag cttccacaga
    35701 accctgtacg tctgccttca ggacgatatt cacttcgtga acttcgcctt cggtcatgtt
    35761 ggcgaacatg ttctcgagtt tagatttctg ctgacgcgcc agtttaactt cgcggaattt
    35821 accctgacga tagagtgcaa cttcacgcgc tttcttctcg tcacgtacaa cggtaacttc
    35881 atcacccgca gccggaacgc cggacaggcc gaggatttcc accggaatgg acggacccgc
    35941 ttccagcact tcctgaccca gttcgttgcg catcgcacga acacgaccgt attcgaagcc
    36001 acacagaacg atatcgccct tgtgcagagt accttcacgt accagaacgg tagcaaccgg
    36061 accacgacct ttatcgagga aggattcgat aaccgcaccg ctcgccatac ctttacgtac
    36121 cgctttcagt tccagaactt ccgcctgcag caggatagcg tccagcagct catcgatacc
    36181 agtacccgct ttcgcagaca catgtacgaa ctggctttca ccgccccact cttccggcag
    36241 gatgccgtac tgggagagtt cgttcttaac gcgatccgga tcggcttctg gtttatcgat
    36301 cttgttcact gcaaccacca ccggaacctg cgccgctttc gcgtgctgga ttgcttcgat
    36361 ggtctgcggc attacgccgt cgtcggcagc aacaaccagg actacgatgt ccgttgcctg
    36421 cgcaccacga gcacgcattg aagtaaacgc ggcgtgcccc ggggtgtcca ggaaggtgat
    36481 catgccgttt tcagtttcaa cgtggtatgc accaatgtgc tgggtaatgc cgcccgcttc
    36541 gccagaggcc actttcgttg aacgaatgta gtccagcaga gaggttttac cgtggtcaac
    36601 gtgacccatg atggtcacaa ccggcgcgcg cggttcagcc gctgcacccg tgtcacggtc
    36661 gctcattacc gcttcttcca actcgttttc acgacgcagg ataactttgt ggcccatctc
    36721 ttcagcaacc agctgtgcgg tttcctgatc gataacctgg ttgatggttg ccattgcgcc
    36781 cagtttcatc atcgctttga tgacctgaga gcctttaacc gccatcttgt tcgccagttc
    36841 gccaacggtg atggtttcgc cgatcacaac gtcacggtta acggcctgag caggcttctg
    36901 gaagccttgc tgcagcgaag aacctttacg ttttccgccc ttaccgccac gtactgctgc
    36961 gcgtgcttct tcacgatcag cttttgattc agcgtgtttg ttgcctttct tcggacgcgc
    37021 agctttcgcg ttacgaccac ggccacggcc gccttcgact tcacgatcgc tttcgtcttc
    37081 tgcctggcga gcatgttgag aagtagtaac gtgataatcg ctggaatctt cagtcggttc
    37141 cgcgttatca gtccatttgt tttcttccgc catacgacgt gcttcttcag caacgcgacg
    37201 tgcttcttct tcgagtttac gacgcgcttc ttcttcggct ttacgcttga gctctgcagc
    37261 ttcctgctca cggcgggctt tttcagcctg ggcgttttta gtcatatcgt cttgttgatt
    37321 gctcactttg tctttttccg cagcttcacg tttcgcttgt tcagcagctt cacgcttagc
    37381 ttgttctgcg gcctcacgtt cagctttttg ttgcgcctca cgtttggccg attcttctgc
    37441 ctcacgacgg gcttgctctt ccgcttcacg ctgcgcttgc tcttccgcag caaggcgttc
    37501 agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga tttgtaccga
    37561 tttgcttttt ccaccggtac caggaatgtt aagggtgctg cgtgttttac gttgcagcgt
    37621 caatttgtcc gggcctgaat ttttctgatt caggtggtca atcaaagtct gtttctcttg
    37681 tgcagacaca gagtcgtcag cagacttccg gatacctgca tcagcaaatt gctgtaccag
    37741 gcgttccacg gaggtctgtc gctctgcggc cagcgtttta atcgttacat ctgtcatgct
    37801 gttccttcct gctacagttt attacgcttc gtcaccgaac cagcaaatat tacgggcagc
    37861 cataatcagt gctccggctt tttcgtcggt caacccttcg atatcagcca gatcatcaat
    37921 gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa
    37981 atcacgatct atcccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct
    38041 ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc
    38101 aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccag
    38161 ttcttccagc gtcgagaagc cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc
    38221 gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag
    38281 gtcgtcaacg gtcatcacgt tgagttccca accgctcagc tgcgaagcca gacgcacgtt
    38341 ctgaccatta cggccaatcg cctgcgccag gttaccggct tcaacggcga tatccatggt
    38401 gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac
    38461 gaactgcgcc gggttatcat cccacaggac gatatcgata cgctcgccgc ccagttcagt
    38521 agaaaccgcc tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg
    38581 tttatcgttg gttttcaccg cgattttcgc acgagaaccc ggatcgcgag ccgctgcttt
    38641 aatttcaatc acttcttcgc cgatttccgg cacttcaatg cggaacagtt cgatcagcat
    38701 ttccggcttg gaacgggtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata
    38761 gagcacgcca cgaacgcggt cgccaggacg gaagttttca cgcggcagca tatcttcgcg
    38821 caggatcacg gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt
    38881 tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc
    38941 acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg tggtgatacg
    39001 gtcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaggttca ggctttcatc
    39061 ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac
    39121 taaccagcga cggaaggtgt caaaatcacc gcttttgcga tcgatctgta cacggacgtc
    39181 gatctcttgc tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa
    39241 aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc
    39301 tttgttcatc gcgggctttt cacctcatcc agactattaa aagtggggaa ccaggttcgc
    39361 cttctggata ttactcagcg cgaacacttc atctttacct tcgacggtaa ctgtgatcat
    39421 ttcaccgtct accgctttga taacgccctg ccatttacga cggttttgta ccgccatgcg
    39481 gagaaccaga gtcacctctt ctccgacgaa acgggcgtag tgttcagccg tgaacagtgg
    39541 gcgatcgaga cccggtgagg agacttccag gttataagca acggtgatgg gatcttcaac
    39601 atccagcaca gcacttacct ggtggctcac atcagcacaa tcatcaacat tgatgccatc
    39661 ttcactatca atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt cgatgccaac
    39721 cagttcaaaa cccagggcct caactggcgc agtaatcatc tctgttaatt tttgctctaa
    39781 tgtggacaag cccaccccca agacataaaa aaagggccta aagcccagtt attctgtagt
    39841 cagataacaa aaaaccccga taaatcgggg ctttatataa ctgaacccta taaccgcaac
    39901 tgcggtctgg agcactttcc agaaggattt tttcaaatcc cactacgaag gccgaagtct
    39961 tcacagtata tttgaaaaag gactctaagg gaaagtggtt gcgggggccg gatttgaacc
    40021 gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg cgcctgaaac
    40081 gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt tggtgtaccg
    40141 agacgggacg taaaatctgc aggcattata gtgatccacg ccacattttg tcaacgttta
    40201 ttgctaatca cgtgaatgaa tatccagttc actttcattt gttgaatact tttgccttct
    40261 cctgctctcc cttaagcgca ttattttaca aaaaacacac taaactcttc ctgtctccga
    40321 taaaagatga ttaaatgaaa actcatttat tttgcataaa aattcagtga gagcggaaat
    40381 ccaggctcct catcagttaa ttaagcaggg tgttatttta tgacgacgat tctcaagcat
    40441 ctcccggtag gtcaacgtat tggtatcgct ttttccggcg gtctggacac cagtgccgca
    40501 ctgctgtgga tgcgacaaaa gggagctgtt ccttatgcat atactgcaaa tctgggccag
    40561 ccagacgaag aggattatga tgcgatccct cgtcgtgcca tggaatacgg cgcggagaac
    40621 gcacgtctga tcgactgccg caaacaactg gtggccgaag gtattgccgc tattcagtgt
    40681 ggcgcatttc ataacaccac tggcggcctg acctatttca acacgacgcc gctgggccgc
    40741 gccgtgactg gcaccatgct ggttgctgct atgaaagaag atggcgtgaa tatctggggt
    40801 gacggtagca cctataaagg aaacgatatc gaacgtttct atcgttacgg tctgctgacc
    40861 aatgctgaac tgcagattta caaaccgtgg cttgatactg actttattga tgaactgggc
    40921 ggtcgtcatg aaatgtctga atttatgatt gcctgcggtt tcgactacaa aatgtctgtc
    40981 gaaaaagcct actccacgga ctccaacatg ctcggcgcaa cgcatgaagc gaaggatctg
    41041 gaatacctca actccagcgt caaaatcgtc aacccgatta tgggcgtgaa gttctgggat
    41101 gagagcgtga agatcccggc agaagaagtc acagtacgct ttgagcaagg tcatccggtg
    41161 gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga tgctggaagc taaccgcatc
    41221 ggcggtcgtc acggcctggg catgagcgac cagattgaaa accgtatcat cgaagcaaaa
    41281 agccgcggca tttatgaagc ccctgggatg gcactgctgc acattgcgta tgaacgtttg
    41341 ttgaccggta ttcacaacga agacaccatt gagcagtatc acgcgcatgg tcgtcagttg
    41401 ggccgtctgc tgtaccaggg acgttggttt gactcccagg cgctgatgct gcgggactct
    41461 ctgcaacgct gggttgccag ccagatcact ggtgaagtta ctctggagct gcgccgcggg
    41521 aacgattatt caatcctgaa taccgtctca gagaacctga cctacaagcc agagcgtctg
    41581 acgatggaaa aaggcgactc ggtgttctcg ccagatgatc gtatcggtca attgaccatg
    41641 cgtaacctgg atatcaccga tacccgcgag aaacttttcg gttatgccaa aactggcctc
    41701 ctctcctcct ctgccgcttc aggcgtgccg caggtggaga atctggaaaa taaaggccag
    41761 taattcgaca tcaaccctgc ccttcagggg tggggttacc ttgcttatcc tctgtaaact
    41821 cctttctgag catcacataa tcgaaaacat catggtttgc atccacaacc cgtgagtctc
    41881 ctttatagtg tgcaatcact tcctccagcg ttttcggttg ttccggcttt gttaccccca
    41941 tccacgcatt aatcaggtaa ttattgtaag ctgtggaaaa gatgttgttt tccgttgtgc
    42001 gatccacgcc atccccaaga acggggctat accagataaa catcgggaca tgatatgcct
    42061 gctggctggc ctccctgcct ccatgaaaat agacgttctt cttcgtaggg tcacgttcca
    42121 ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc ttttaataat tcaaaaacct
    42181 gacctagcaa actatctgtg taatgaatgg agttgtcata gcaggcatcc tgatcgtcct
    42241 gcggttgaaa cacggcgctg gattgcggat aggcgctaca agccggttca tggcttccat
    42301 ttaaatgaag aacaatcagt ttcttttgct gcgtattttg ctgtaatgct tgcgataaat
    42361 gcggcaacaa caattcatca aatcctctga cataaactgt ttccatggcg cgcatggcga
    42421 tactggtaac tgctgtacca ttctgccgaa aagcggattg cgagcttagc cagaaagtct
    42481 gaaatcctgc ttgattagcc atattaataa tgttgtccgg gtaattatga atgtcatgac
    42541 tcaaaacgga atcagcagtt aaagaaaggg gaaccgacag cgcagtgtaa ggtgcaccgc
    42601 ttattgcttg attaaacagt ttgatctgtt ttctttgtgc ttcgacttgc ggtgtcgtag
    42661 agcgtgtata tccatacaaa gacatattgt cgacgcgtac agactccccc acaatcaaca
    42721 cgtaggtatc aatacctgta tccctgactg ataattgaaa atacggcacc gtgtttgcaa
    42781 ttgagagtaa tctttgatgc tctttcgctg ctaaagcaaa ataattgaga ttgaaaaacg
    42841 gtgtataggt agcaaatcgc gacgctagta tatatggact gaacgctttt ttattttttg
    42901 catctttata agcaaattga caagcggaaa ataaactgcc cgaaatgaca atcagcaata
    42961 atattcctgt cacttttttc gtcggcaagg aaacatcata ttttattatt actgccaaaa
    43021 aaagaaggga taaaaaggca aaagcacata gataagggat atacatcccc agcattttga
    43081 caacttcatc cggatcactc tgcagcacac taatcgcaaa tccatcattg aaagttgtac
    43141 caaaggtaca ccatgaatag aggctaatac tcatgtctgc cgctgtcagt aacacaaatg
    43201 gtgcggcaac aagcaggcga agcggaaggg atgatatacg tttaacaacc aggatggtca
    43261 gaacgaagaa cagcaatcgc tgaaccatat gcgctccgga agaggcgact aagttcgtaa
    43321 taccaaaaac aataacacaa agatacaaca accaatgaga tttaaaactt ctaacaaatt
    43381 tgttgaatac tgtcatccag aaatccaagt tcacaaccaa tataaacaat tatcaacaat
    43441 gttcaatttt atatcagaca tgtttttcaa ctctgatcca tatcagaccg aaacactgtt
    43501 agttttttct ttttaaatga tgcggccatt gttctcagta attaaataac attatcaatg
    43561 cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg
    43621 tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg
    43681 ccaattatgt cagcagaagg gaaaagttta taactaaggc gtaccgatac catcgtttca
    43741 aagtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca
    43801 gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg
    43861 aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta
    43921 ccaattccac cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt
    43981 agccggagct gctggctgaa tttgttcggt tttcgccggc gcactcaggt tttcccattc
    44041 gctaccttta ttggttttgt tgctattgat gttacccagc accagactga tgatgaagaa
    44101 taacgttgcc agcagcgctg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa
    44161 cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg
    44221 cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacta aaagagcttc
    44281 gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc
    44341 gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaagc gaatgacctt
    44401 cgcaagggca attttattgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt
    44461 tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca gttatttatc gttttaaacg
    44521 gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct
    44581 tccaccatca cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg
    44641 ctacccagcg cagcttcaac ttctgcggtc acagctttga ctgactcatg ctccagcgga
    44701 tcgccgctgc ctgctgtgta acgcacgtta accagaatct gcgggaacat tttcatgccg
    44761 ctgcaaaggt cgtgcaggct catatggtta cgcgccatcg ccgccagcac ctgcaagcca
    44821 gcaacaatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc ggagttctct
    44881 gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc
    44941 gcgcgcgcaa atggaatacc taactgtttc agcgccagtt caagccccat gttgctcatc
    45001 aatgtaccca cagcaccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata
    45061 tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg
    45121 ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc
    45181 gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgttcgg ctcacaaccg
    45241 atagcgataa cgttcgcccc cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca
    45301 ccgtttgcac aatccaccac aatcttcagt tcactgaggc taagttcgtt cgggaacgtg
    45361 gctttgcaaa actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt
    45421 tctgccgaat caacgcagct gatctctttt tccatttccg cttcgatggc ctcttctacc
    45481 gcatccggta gtttggtgcc gtcgatagag aagaatttaa tgccgttatc gtagaatggg
    45541 ttatgcgatg cagaaattac aattccagcc tctgcgcgga aggtacgcgt cagataagcc
    45601 acggccggtg ttggcatcgg gccagtgaag agcgcggaaa gaccggctgc cgcaagcccc
    45661 gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc aataataatc
    45721 ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa
    45781 tcaggggtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta
    45841 cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc
    45901 ttctacggtt tctttgacgt catgaacacg aatgatgtgc gcgccttgca ttgcggcaat
    45961 gaccgcacag gccagactac cgctcaaacg ctcggacggc cccacgttca gcagctgtcc
    46021 aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc
    46081 caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc
    46141 gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa
    46201 gtagcgattc acttctgcaa agacatcgtg atacttcggg gcttcctgca tggtttttgg
    46261 atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc
    46321 gccaggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc
    46381 acggatgact tctggtttgg atgtatctac cgagatccag acttcgaagc gttgagcaat
    46441 tgcctcaacc acaggaataa cacgttgtaa ctcttcttca acgctaactt ccgccgcccc
    46501 tggacgtgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt
    46561 cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg
    46621 cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc
    46681 aaagagtttc ataatgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc
    46741 ccggggtttt tggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc
    46801 tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt
    46861 ggactaccat tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga
    46921 cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc
    46981 atgagagcat ctttcatcgc atgcagtata tccatattgt cggtcagaag ctgacgcgca
    47041 cgattatagt tgcgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca
    47101 tcggacatat gtttcgcctt cgctacgcta cggccgagga acacttcacc ttcttcttcc
    47161 gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat gttacgtgcc
    47221 aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag
    47281 atgatctctt ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga
    47341 cggctagcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg
    47401 cgtgggataa tcgtcacttt gtgcaccgga tcatgttccg gcaccaggcg accgataatc
    47461 gcatgacccg cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag
    47521 cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca
    47581 acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca
    47641 gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat
    47701 ggtacgcgac gcatgtgaac tttcaggatc tgttcacgac cgcgaacatc tggcaagcca
    47761 accacaacct gacggtcgaa acggccagga cgcagcagcg ccgggtcgag aacgtccgga
    47821 cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc
    47881 agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc tgcgccacgc
    47941 tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc
    48001 tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca
    48061 gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcaac
    48121 gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt
    48181 ttctggaagc ggctcggctc gcgcagatac tcaaccagtt cagcaacttc ttcttttgct
    48241 tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttcagt cagcatgcgc
    48301 gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg
    48361 aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc
    48421 agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaagtta
    48481 tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg
    48541 gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct
    48601 tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg
    48661 aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca
    48721 ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc
    48781 cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca
    48841 atatacactt cccgcgaacg tgcgcgagaa gagtccggct tacgaacttt gaccttcgta
    48901 aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact
    48961 acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga
    49021 tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac
    49081 atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca
    49141 tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg
    49201 caagcgatga tacggccttt accgccaatt tgggtgacca catattgtga ccaaccaccc
    49261 ggcgcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt
    49321 atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca
    49381 tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct
    49441 gtcatttaac tttcccattg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc
    49501 ctatttggcg atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc
    49561 aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc
    49621 gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat
    49681 tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga
    49741 aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt
    49801 catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg
    49861 ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca
    49921 ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt
    49981 tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat
    50041 tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt
    50101 gccgatcagg ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc
    50161 ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt
    50221 tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg
    50281 cgcgttcgac agcttggctt cgatgtcttt gatacggcct tcgcagaagc cctgctgttc
    50341 acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat
    50401 agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt
    50461 ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct
    50521 gatggggcaa gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg
    50581 gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt
    50641 ttgatagtca ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc
    50701 gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt
    50761 tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta
    50821 cattactcaa ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc
    50881 ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta cgcagaaagt
    50941 gattactgca ctggcggcgt tgattcaact cggccccgat tttcgtttta ccacgacgct
    51001 tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg
    51061 tgccgatccg acgttaagac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc
    51121 tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga
    51181 taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc
    51241 cgccatagtt gaccgcaact gtttctccgt ctcgctctat agtgccccaa aacctggtga
    51301 tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac
    51361 cctcccccgt ggttctgccg aagcgcaata ctgcgagctg gatgtggtgc caggcgacct
    51421 gaaccgcttt acgctgaccg gatgcctgcc acaacgttct gagccgctcc cgttggcttt
    51481 tgccgtgcag gatggtgcca gctatgctgg agcaattctg aaagatgagt taaaacaggc
    51541 aggtatcacc tggagcggaa cactgctgcg tcagacgcag gttaacgaac ccggaacggt
    51601 agttgccagt aaacagtcgg ccccgttgca cgatctgctt aagattatgc tgaaaaagtc
    51661 ggacaacatg attgccgata cggttttccg catgatcggc catgcgcgct tcaatgtgcc
    51721 tggaacatgg cgggccgggt cggacgccgt gcgtcagatc ctgcgccagc aagccggtgt
    51781 cgatattgga aataccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc
    51841 tcccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt
    51901 tatctctatg ctgccgctgg cgggctatga cggctctttg cagtaccgtg caggtctgca
    51961 tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa
    52021 cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcgtttgtgc aatatctttc
    52081 tggctatgca gtagaaccgg cggatcagcg taatcgccgt attccgttgg tacgttttga
    52141 aagccgtttg tataaggata tttaccagaa caattagtct aaagagaaac cccggcacat
    52201 ggccggggtt ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg
    52261 tcccagtcgt catcccagtc ttcatcatcc tcttcagcaa tctcttcaag ctgctggcga
    52321 tgataatcat cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg
    52381 accgggtttt caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtccg
    52441 ctcgccgcag agatcagata atatttatct tcccaaccca gcgcctcagc gatcgctttc
    52501 gctttctctt cggcttctgc cttatccagc aggtcgatct tgttgaagac taaccaacgc
    52561 ggtttcgccg ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt
    52621 tcaaccggat cggtgccatc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa
    52681 cgctccaggt gcttcaggaa gcgaatgccc agacctgcgc cttctgcagc gccttcaatc
    52741 agtcctggaa tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacaccaaga
    52801 cttggcacca gagtggtaaa cggataatcc gccactttcg gtttagccgc cgatactgcg
    52861 cggataaatg tcgatttacc cgcgtttggc atccccagca taccgacgtc agccagcagc
    52921 atcagctcca gtagcagctc acgcttatcg cccggcgtac cgttagtttt ctgccgtggt
    52981 gtacggttaa ctgacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca
    53041 accagcagac gctgaccgtg tttggtcata tcgcccatgg tttcaccggt accctggtcg
    53101 ataacacgcg tacctaccgg cactttaatc gtcacgtctt taccgcgctt accggtacag
    53161 tcgcggcttg cgccattctg accacgctct gcgcggaaag atttttcaaa acgataatcg
    53221 ataagcgtgt tcaggttctc gtcggcttcc atccatacgt caccgccgtc accgccgtcg
    53281 ccgccatccg ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta
    53341 ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc
    53401 gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc
    53461 caaatacgat gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga
    53521 taacctaaaa ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga
    53581 tctgaaaaaa acagcgtaaa cagtggggtg agcgtaatga tcgcgctcac ctgcgctgcc
    53641 tgccagcgag ccatcgcttc cgccagggcg ccatatccta ccaatgtatt cagtccgcaa
    53701 aaaattaaac atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga
    53761 gggaagagcg caattgtaca taaagtgtac agcaaaaaca ggatctgcgg tgaggccagt
    53821 cgacgcaata aaaccttttg cgccacgcca taactcaccc acaccgtcgc cgcaccgacc
    53881 ccaaagatta ctccccaggt gtaatcggtg agctttgtaa atatctcgac cagactggtg
    53941 ttaaaaaaca tcaccaggcc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc
    54001 attttctctt tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca
    54061 atcacctgcg aagcggtcgg acccaggtat tgcaaggatg agctgaacag gatgaagttc
    54121 ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg
    54181 cgtaatggcg gcaaccgctt cttcaccgca agaatggcac ccaggccaat actcgccatc
    54241 aagaaacggt aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg
    54301 attggcaacg ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc
    54361 tgctgcttca taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc
    54421 atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt
    54481 accgggacgc gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct
    54541 ttaacttcga atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa
    54601 cctacgttag cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc
    54661 agaacggatt cgccaccgaa acgcttaacg cccaggcgtt tagcttctga atcgcgaccg
    54721 ttacgtgtgg agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg
    54781 cgctgatgcc agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat
    54841 agtgtttacg acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa
    54901 cttcagcttt gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt
    54961 ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt
    55021 ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg
    55081 cgtacatata aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa
    55141 tattcacaat agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca
    55201 atacacgaag aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt
    55261 acaatcaccc tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat
    55321 cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca
    55381 agatatggcg ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccaactgat
    55441 caatcagtta ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc
    55501 tgtactggct gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct
    55561 gatcgagttt atccacacgg cgactctgct gcacgacgac gttgtggatg aatcagatat
    55621 gcgcaggggt aaagctaccg ccaacgctgc atttggcaat gccgccagcg tgctggtagg
    55681 cgattttatt tatacccgcg cgttccagat gatgaccagc ctcggctcgc tcaaagtgct
    55741 ggaagtcatg tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa
    55801 cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc
    55861 gcgtctgttt gaggctgccg cgcagtgttc cgggattctg gctggctgta cgccggagga
    55921 ggagaaaggc ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga
    55981 tttactcgat tacaatgccg atggcgaaca gttaggtaaa aacgtcggcg acgatctgaa
    56041 cgaaggtaaa ccgacgctgc cgctgctgca tgcaatgcat catggcacac cagaacaggc
    56101 acagatgatt cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct
    56161 ggaagcaatg aacgcttgtg gatctcttga atggacgcgt cagcgtgccg aggaagaagc
    56221 agacaaagcc atcgcagcgt tacaggtgct cccggacacc ccgtggcgag aagcactcat
    56281 cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag
    56341 cgcgcgggat gattccagta aattccataa aaacacttat tcagctctat aacacctgaa
    56401 aaatagggac gtacatcttt atatattctg aatattcaca ctctttacag gaacttttta
    56461 gagcaatagg ccatcaggag tatagtgatg ctcgacagaa gaagtgttct gaatgaaagc
    56521 gactaactta aggagtgagg aaagatggaa agtaatttca ttgactggca tcccgctgac
    56581 atcattgcgg gtttgcgcaa gaagggaacg tcaatggcgg cggaatctcg cagaaatggt
    56641 ttgagttcct caacgctggc gaacgcatta tcgcgcccct ggccgaaagg agagatgatt
    56701 attgcgaaag ccctgggaac tgacccctgg attatctggc catctcgcta ccatgatccg
    56761 cagacccatg agtttatcga cagaacgcag ttgatgcgta gctacactaa accgaaaaaa
    56821 tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc tctcagacaa ttactcgcct
    56881 ttcacacgct caatatttgc acctaaagcg cgcagtttgt cttcaatgcg ttcgtagcca
    56941 cgatcgatgt gataaatacg atcaactacc gtcgtccctt ccgcaataca gccagccagc
    57001 accaggcttg ctgatgcacg cagatcggtt gccataacct gtgcgccaga aagtttttca
    57061 acaccgtgac aaataacggt attgctttcg atttcggcgt gcgcgcccat acggctcagc
    57121 tctggcacat gcataaagcg gttttcaaag accgtttcgg tgatgaagcc ggtcccttct
    57181 gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc cggatgcggc
    57241 gcggtacgta cgttaacagc cttcggacgt ttgccatgca tatccaggct aatccagtct
    57301 tcgccgactt cgatgtctgc tccagcgtca cgcagtttcg ccagcacggc gtcgagagta
    57361 tctggctgcg cgttacggca gataattttg ccgcgagaga tcgccgccgc caccaggaaa
    57421 gtaccggttt cgatacgatc cggcagcacg cgatagacac cgccgcctaa acgttccaca
    57481 ccttcgatga cgatacgatc ggtgccctga ccgctaattt tcgcacccag cgtaatcagg
    57541 aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt tttcaataat cgtggtgcct
    57601 tccgccaggg ttgcagcaca catgatggtc accgttgcgc caacgctgac tttatccatc
    57661 acgatatgtg cgcctttcag acgaccatcg acggaagctt taacgtaacc ttcttccagt
    57721 ttgatggtcg cgcctaattg ttcgagacca gaaatgtgta gatcaaccgg acgtgcacca
    57781 atcgtacaac cgccaggcag tgaaacttgc ccctgaccaa agcgcgctac cagcggcccc
    57841 agcgcccaga tagaagcacg catggtttta accagatcgt aaggtgcgca gaatacatta
    57901 acgtcgcggg catcaatatg cacagaacca ttacgttcta ctttcgcacc cagctggctt
    57961 agcagcttca ttgatgtatc gacgtctttt agtttcggga cgttctggat ctctaccggt
    58021 tcttccgcca gtagtgcggc aaaaaggata ggcagagcag catttttagc gccggaaatt
    58081 gtgacttcgc cctggagctt cgttggcccc tgaacacgaa atttatccat ttagtttgtt
    58141 ctcagttaac aattcatatc cgctaccggc gaatcgccca tagctcaaaa gccgttcagt
    58201 ttgcgatcgc gcgcccactc cgcaggggta tacgctttga tcgacacagc atgaatgcgg
    58261 ttatccgcaa tatattccat cagcgggcca tagaccgtct gctgtttttt aacccgactc
    58321 atgccgtcaa acaactcacc cacggcaata acctgaaagt ggctgccatc gccggaaacg
    58381 tggacttcct ggagggagag agcgttcatc aacacgctct gaatttcatt attttccatg
    58441 ggatcttcaa tcatcagtta ataaaccagc gaaacatctt agagcaaagt tgcgctggca
    58501 taaataagca aaaagcctcg ctgataaatc agacaaggct cgacttgcag gcaggtttgc
    58561 cggacaggcg gttaacgcca tatccggcct gaaaaaattt aacgaggcag aacatcagca
    58621 ggcaaattat acaatttcgc cagggtatac actttgtcgt ttaccccctg aagcgtcaca
    58681 ttgttgccct gctttttcgc cagatcgata agatggagca gcagcgccag tccacctgta
    58741 tcaacgcggg atacacggct aagatcgata caggtaatcc ccttcaccgc ttcctcacgc
    58801 atttcccaaa gcggtagcag aacgtcctga tccagctctc cggataacgc cagcgtgtca
    58861 cccgtctgca tccagctcag tgactcgctc attatttttt ctcttccaga gtgattttct
    58921 gttgagaaat cgatttcagt tgcgcagtca ggccgtcaat acctttggta cgcagcagcg
    58981 ttccccactc gttttgtttg gtggtgatca tactgacgcc ttcagcaatc atgtcgtaag
    59041 cctgccagtt gcccgtctgg gagtttttac gccactggaa gtccagacgc accggcgggc
    59101 ggccattcgg gtcaataatg gtaacgcgaa taggcacaat ggttttatca cccagcggct
    59161 gttctggcgc aatctgatag gtttgaccgt gatacatcgc cagcgcctga ccgtaagcct
    59221 gcttcaggta ctcacggaaa gcggcaaagt aagcatcacg ttgagctggg gtcgcactct
    59281 tgtaatactg acccagcacc agcgcaccgg cgtatttcac ctgtacgtat ggcagcagtt
    59341 cctgatcaac aatggtacgc agataatccg ggttggcccg aatttgtggt tgctcattct
    59401 tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag cttatacgga ttggtctggt
    59461 ctgccgcggt tgccgcactc agcggtgcaa tcaccagcaa agcgaccatc attaaacgtt
    59521 taaacatgcg tcggttctcc tgaaattatt tcgttgtacc cacaggttca gtggtttcat
    59581 tattacctgg cgcagcagct ggcgcatcgc cactattctt attgtcatcg cctttactac
    59641 cgtaaaggaa ctgaccaata agatcttcca gcaccatcgc agacttggtg tcctgaattg
    59701 tatcgccatc cttcaggata gcagtcccca gttccgggtc ttcaaaaccg acgtttaatg
    59761 ccagatattg ttcccccagc aggccggaag tacgaatgct cagcgaactg gtatcaggaa
    59821 tgtggttata acgttgttca atttccagcg ttacgcgcgg cagataggtt ttcgggtcca
    59881 gcgtaatatc cgccacccgg cccacaacaa caccaccaat gctgaccgga gagcgcgctt
    59941 tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta ggtcggttca gtacgtatgg
    60001 acgtcacgtt cgccgccttc aggcaaacaa acagcgccgc cagtagtgct gctaataaaa
    60061 agatacccac ccaaatttca ttttttttcg tttgcatgaa ctcaattccc aaacatcaat
    60121 gcggtcagca caaaatccag ccccagaaca gccagagacg agtggacaac ggtgcgagtg
    60181 gttgcccggc taatcccggc agacgtcggg atggcgtcgt agccgttaaa caacgaaatc
    60241 cacgtcaccg tgatggcaaa caccacgctc ttaatcagac agttgaccag atccatacgc
    60301 cagtcgacgg cattttgcat tgccgaccag aagaacccgc tatcaatgcc tttccaactg
    60361 acgccgacca gagatccgcc ccagatcccc acggcgacga aaataaccgt caacagtggt
    60421 aatgaaataa ccccagccca gaaacgggga gaaataaccc gacgcagcgg atccaccgcc
    60481 atcatctcca tactggagag ttgctctgta gcgcgcatca ggccgatttc tgcggttagc
    60541 gccgaaccag cacgcccggc aaacaacaac gcggcaacca ccggccccag ttcacgcagt
    60601 agcgataacg ccaccagcat acccagactg gtttccgcac tataagtggt cagaaccaga
    60661 taaccttgca gccccaacac cattccgatg aacacgccag aaaccacaat aatcagcatc
    60721 gacaggacgc cgacattata gagctggcgc accagcagcg gcgcatgttt gcgaaattcc
    60781 ggtttgccga ccagcgcatt gaataacatt aacccggccc gcccgaacgt tctcagggtt
    60841 ttaatccctt tatgtccgag cgacgccagc gcatttaaca gcatgagtgg cttaactccc
    60901 tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg aacggaacag gcccgtcagc
    60961 tatcccgtcc agaaactgac gtacgcgtgg atcaggattc gcctgcaacg cctgggcact
    61021 gccatgagcg acaatttttt tgtccgccag gatccaggcg tgatcggcaa tacttaacac
    61081 ttccggcaca tcgtgagaaa ccaccacaca agtcacaccc agcgcgctgt tcagctcaga
    61141 aatcagcttc accagtacgc ccatggtgat gggatcttgc ccaacaaagg gttcatcaaa
    61201 catgatgaga tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc gcgccatccc
    61261 accggaaagt tcagaaggca ttagtttagc cgctccacgc agccccacgg cctccagctt
    61321 catcatcacc gtactatgca acaatggcgc gggaagttgg gtatgttcgc gcagtggata
    61381 ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc cctgactgaa ataacatgct
    61441 catccgtttg cgcactgtat acaggcgcga acgagacatc gccggaatat tctcaccatc
    61501 gaaaagaatc tcaccatgat ctggtgcgat ttgcccgcca atcagacgga gtagcgtcgt
    61561 tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca cggtcaggga
    61621 aatattatcg aagatgcagc gattgccacg cgtaaaactg acatcgcgca tatcgactaa
    61681 attcgccaca gactgctcca taattcaccc ttcgtcttgc gttgattttc taagcatggc
    61741 gctcaattta accttgaacc caacatattt acagaatatt acccgccgtg gttagcgaaa
    61801 gctggcattt gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc gttacggctt
    61861 tctgaaaatc ttcagcggac cggcgagtat acctgaagaa aggacgttag atgcttttag
    61921 ctacggcact gttaattgtt ggtttacttt tggtcgttta cagtgccgac cgcctggttt
    61981 ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc gctaatcatc ggcatgacgg
    62041 tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc gcttgccgcg tctctgcacg
    62101 aacaacgcga tttagccgtt ggtacagccc tcggctcaaa cattatcaat atattgctga
    62161 tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca ttctgatgtt ctacgccgtg
    62221 aattaccctt aatgttgttg gtcagcgtgg tggccggttc cgtactctat gacggacaac
    62281 ttagtcgaag cgatggtatc tttctcctct ttctggctgt gctatggctg ctgttcattg
    62341 ttaaacttgc acgtcaggct gaacgtcagg ggactgacag cctgaccaga gagcagcttg
    62401 cagagctgcc tcgtgacggc ggattgcccg tcgcgttttt atggctcggc attgcgctta
    62461 tcatcatgcc agtggccacg cggatggtgg ttgataacgc cacggtgctg gcgaattact
    62521 ttgccatcag cgagttgacg atgggtctga cggcaattgc tatcggaacc agcctgccgg
    62581 aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa cgacattgct gtcggaaata
    62641 tcattggcgc aaacattttt aatattgtca tcgtattggg tttacccgcg ctgataacgc
    62701 caggagagat tgatccactg gcgtacagtc gtgactacag cgtgatgttg ctggtgagca
    62761 ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc gggccgtggt gtaggggtat
    62821 tattaactgg cggatttatc gtatggctgg cgatgttgta ctggttatcg ccaatactcg
    62881 ttgaataact ggaaacgcat tatgtcgcac gtagagttac aaccgggttt tgactttcag
    62941 caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct tgatcaatac
    63001 atcaatcaga atttcacgct tgcctgtgaa aagatgttct ggtgtaaagg gaaagttgtc
    63061 gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa tggccgcaac gtttgccagc
    63121 accggtacac cttcattttt cgtccatcct ggtgaagccg cgcatggtga tttaggcatg
    63181 gtcaccccac aggatgtggt gattgctatc tctaactcag gtgaatccag cgaaatcacg
    63241 gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa tctgcatcac cggtcgcccg
    63301 gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc gaaagaagcc
    63361 tgtccgttag ggctggcacc gaccagcagc accaccgcca cgctggttat gggcgatgcc
    63421 ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg aagattttgc gctctcacac
    63481 ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa acgatattat gcatacgggc
    63541 gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct ggaagttacc
    63601 cgcaaaaatc ttggtgtgac tgtcatttgc gatgacaata tgatgattga aggcatcttt
    63661 accgacggtg atttacgccg tgtcttcgat atgggcgtgg atgttcgtca gttaagtatt
    63721 gccgatgtga tgacgccggg gggaatacgt gtgcgccctg gcattctggc cgttgaggca
    63781 ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg cgaccattta
    63841 ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg tagtgtaaag attcaaggat
    63901 aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct gtcagcgccg
    63961 acgttatagc aaaagcagag aacattcgtc tgctgatcct cgatgtcgat ggcgtactgt
    64021 cagatggcct gatttatatg ggcaataatg gcgaagagct gaaagcgttc aatgttcgtg
    64081 acggttatgg cattcgttgt gcgctcacct ctgatattga agtcgctatc attaccgggc
    64141 gaaaggctaa actggtagaa gatcgttgtg ccacattggg gatcactcac ttgtatcagg
    64201 ggcagtcaaa caaactgatc gcctttagcg atttgctgga aaaactggcg attgccccgg
    64261 aaaatgtggc ttatgtcggc gatgatctca tcgactggcc ggtaatggaa aaagtgggtt
    64321 taagcatcgc cgtggccgat gcgcatccac tgttaatccc gcgcgccgat tacgtgacgc
    64381 gcattgctgg cggtcgtggt gcagtgcgtg aagtttgcga cttattactc ctggcgcagg
    64441 gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt gggttatcat
    64501 tgtgctgtca ctggcggttc tggtgatgat cggcattaat atggccgaaa aagacgatac
    64561 cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa agcgagcata cggacacgct
    64621 cgtctataac ccagaagggg cactaagcta tcgattgatt gctcaacacg ttgaatatta
    64681 ttccgatcag gccgtttcgt ggtttacgca gccggtactt accacgtttg ataaggataa
    64741 aatcccgaca tggtccgtaa aagcagataa agccaagctg accaatgacc ggatgctcta
    64801 tttatatgga cacgttgaag tcaacgcact cgtgccggac tctcaacttc gcagaatcac
    64861 gacggataac gcgcagatca atctggtgac gcaggatgtt acctctgaag acctcgtcac
    64921 gttatacgga acaacattta actccagcgg tctgaaaatg cgcggcaact tacgcagcaa
    64981 gaacgccgag ctgattgaaa aggttagaac atcctatgaa attcaaaaca aacaaactca
    65041 gccttaatct tgtgcttgcc agctcacttc tggccgccag cattccggca tttgccgtaa
    65101 ccggagacac tgatcagccg atccacattg aatcggacca gcaatctctt gatatgcaag
    65161 gcaacgtggt tacctttacc ggcaatgtca tcgtcaccca gggcaccatc aaaattaatg
    65221 ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg taaagaagtg attgacggct
    65281 acggtaaacc ggcaacgttc taccagatgc aggacaacgg taaacccgtt gaaggtcacg
    65341 cttcacagat gcactacgaa ctggcaaaag attttgtcgt tctgacgggt aatgcttatc
    65401 tgcagcaggt cgatagcaac attaagggcg ataagatcac ttacctggtg aaagagcaga
    65461 aaatgcaggc tttcagcgac aaaggcaagc gcgtaaccac cgttctggtg ccgtcgcagc
    65521 tgcaggacaa aaacaataaa ggccagaccc cggcacagaa gaagggtaat taattcgtta
    65581 tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc gtggtagaag
    65641 acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca aacggtgccg
    65701 gtaagaccac cactttctac atggttgttg gcattgtgcc gcgcgatgcg ggcaacatca
    65761 ttattgatga tgacgatatc agtctgctgc ctctgcatgc acgcgcgcgc cgcggtatcg
    65821 gctatctgcc gcaggaagcc tccattttcc gtcgcctcag cgtttacgat aacctgatgg
    65881 cggtactgca aattcgtgac gacttgtctg ctgaacaacg tgaagaccgc gcgaacgagc
    65941 tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca ctctccgggg
    66001 gtgaacgtcg ccgtgtagaa attgcccgtg cactggctgc gaatccgaaa tttattctgc
    66061 tcgacgaacc gtttgccggg gttgacccga tctcggttat cgacattaaa cgcatcattg
    66121 agcacctgcg cgacagcggc ctgggcgtac tgatcactga ccacaacgtg cgtgaaacac
    66181 tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc cacggcacgc
    66241 ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa gacttcagac
    66301 tctgataggg tagaagtttg cgatgtttta gcaggagagt acgattctga acatgaagca
    66361 aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagctcc aacaggcaat
    66421 tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg cgctggagag
    66481 taatccgctg cttgagcaaa tcgacactca tgaagaaatc gacacccgcg aaacgcaaga
    66541 cagtgaaaca ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg aagagctgcc
    66601 gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca ccagcggtga
    66661 ctacattgac gacgagctgc cggtctatca gggcgaaacg acgcagacct tgcaggatta
    66721 cctgatgtgg caggtcgagc tgacaccgtt ttccgacact gaccgcgcta ttgctacctc
    66781 tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag atattctcga
    66841 aagtatgggc gatgaagaga ttgacatcga cgaagttgaa gccgtcctta agcggatcca
    66901 acggtttgat ccggtcggtg tggcggcaaa agatctgcgt gactgtctgc tgatccaact
    66961 ctcccaattc gataaaacca cgccatggct ggaagaggcc agactgatca ttagcgatca
    67021 tctcgatctg ttagccaatc acgacttccg cactttaatg cgcgtcacgc ggctgaaaga
    67081 agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc ccgggcagtc
    67141 gatccagacg ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta agcataacgg
    67201 tcactggacg gtagaactca acagtgacag cattccgcgt ctgcaaatca accagcacta
    67261 cgcctcgatg tgcaataacg cgcgcaacga tggtgacagc cagtttatcc gcagcaatct
    67321 gcaggatgcc aaatggttga tcaagagcct ggaaagccgt aacgatacgc tactgcgcgt
    67381 gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggcgaag aatatatgaa
    67441 accgatggta ctggccgata tcgcccaggc tgtcgaaatg catgaatcga cgatatctcg
    67501 cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga agtatttctt
    67561 ttccagtcac gtcaataccg agggcggcgg cgaagcttcc tccacggcga ttcgtgcgct
    67621 ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca gcaagttaac
    67681 ctctttgctg tcggaacaag gtatcatggt ggcacgccgc actgttgcga agtaccgaga
    67741 gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca accgataagg
    67801 aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag gcactgcgcg
    67861 aatttgttac agccaaattt gccaaacttg agcaatattt tgaccgaatc aaccaggtct
    67921 atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg catgtaaacg
    67981 gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt gatggcttaa
    68041 ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa cactaattgt
    68101 ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca tttgtacggt
    68161 taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa attatgacaa
    68221 ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg cgaagccgcg
    68281 tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg gcgaaacaac
    68341 ttagtctgcc gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa atgggcagta
    68401 ccggtatcgg caatggtatt gccattccgc atggcaaact ggaagaagat actctgcgcg
    68461 ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatt gacaaccaac
    68521 cggtagattt actatttgcg ttgctggttc cggctgacca aaccaaaacg cacctgcata
    68581 ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt ttacgcgcag
    68641 cccagagcga tgaagagctg tatcaaatca ttacggatac cgaaggtact ccggatgaag
    68701 cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac atggtactga
    68761 tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg ctggaagata
    68821 tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg gctcgaaccc
    68881 tggccgatcg cgagatttct gccgccgtca gcattgatgt tcgtaatatg ccggagtcac
    68941 cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg caactcctgt
    69001 tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc cgactgcatc
    69061 cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc gatttgctgg
    69121 agcctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc gttcacgagc
    69181 tggcagaaat gctgcgtacc cgtctgctgg gtaaacgtga acgtgaactg accatggtct
    69241 ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc tttgacgtgc
    69301 gcttcttgcc gaacccgcac tgggatccga aactgcgtcc aatgacaggt cttgataaac
    69361 ctgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac cagacgcgaa
    69421 gctatcttga gctatggtta cctatgctgg aaaccaacaa ccgtagctac ctgacggtcg
    69481 ccattggttg taccggcggg aagcaccgtt cggtgtatat tgcagagcaa ctggcagact
    69541 acttccgctc gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa aaacgtaaac
    69601 catgaccgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg cccggcctgc
    69661 aatgaagctg tttgaattaa tgcaaggttt tgacgcagaa gtgctgttac gtaatgacga
    69721 aggtaccgag gccgaagcca acagcgttat tgcactgctg atgctggatt ctgccaaagg
    69781 acggcagatt gaagttgaag cgaccggtcc acaggaagag gaagcactgg ccgccgttat
    69841 cgccctcttt aattctggtt ttgatgaaga ttaatcttca tcactttgac attcaaatcc
    69901 cttcaagctc ccccgacaat aagaaaagcg cgtgctgaaa tcgttctcaa tcaacgtcat
    69961 ttgtacattt tgtgcgcttt tcactttcag aagaacctta agaaaacctt aagaagcatt
    70021 gtttaggttt cgtttaagtt aatcgaccat actggagatc gtcagaaaat atttccagga
    70081 gatggcatga ttcgcttatc tgaacaaagt cccctgggca ccggaagaca tcggaaatgt
    70141 tatgcgcatc cggaagatgc ccaacgctgt atcaagattg tctaccatcg tggcgatggt
    70201 ggcgataaag agatccgccg tgagttaaag tactacgcgc atcttggtcg ccggttaaaa
    70261 gactggagtg gaataccgcg ctatcacggt accgttgaga cggattgcgg aaccggttat
    70321 gtctacgatg tgattgcaga ttttgacggc aaaccctcca ttacgctgac cgaatttgct
    70381 gaacaatgcc gttacgaaga agacattgca caactgcgcc agctactgaa acagctgaag
    70441 cgttatttgc aggataaccg tatcgtgacg atgtcgctga agccgcagaa tatcctctgt
    70501 caccgcatca gtgagtcaga ggttatcccc gtcgtctgcg acaacattgg tgaaagcaca
    70561 ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga gcggttatgg
    70621 aaacgattta tcgcccaacc ggcgctggct attgccctgc agaaagatct gcaacctcgt
    70681 gagagcaaaa cgctggccct cacttcgcgc gaagcttaat ccagctggtg ctgttgcata
    70741 aacgactcac cgcctaactg atacatctgc cgtaaaatcc acgcctgacg gctacgcacg
    70801 tagcccgatg gcgcggagac tttgaaacga agtggattag gtaatacagc agccagtaat
    70861 gcagcttccg agcgggtaag tttgctcgcg ggtttgtgga aataacgttg tgccgcagct
    70921 tcgacgccaa acacaccgtc gccaaattcg gcgatattca ggtaaaccgt caggatacgc
    70981 tttttgctcc agactgtttc tatccccagc gttaatccag cttccagccc ttttcgtacc
    71041 cagctacgcc catcccataa aaagagattt ttggccgtct gctgagaaat cgttgaagca
    71101 ccgcgaatac ggttttcatt gcgctcgttg tgcgccaggg ctttctcaat ggaagcgaca
    71161 tcaaagcccc agtgctcagg aaatttctga tcttctgcgg caatcaccgc cagtcccatc
    71221 cacggcgaga tttgatccat actgacccag tcagaatgtg ccacgtaacg aaaattgcca
    71281 tgcagccagg cgctgacctg tcgctcgacc attaccgctg agaagggaac aggcgcaaca
    71341 ctaaacaacg cgatgccccc gccccagaaa acggcgagga caaccattaa ccgcaaaaga
    71401 aaacggcgaa cgaaactaaa caccgttaag cggcttttac tcattcagcc agaaccagca
    71461 cgcgggaaac cagcttatca atgccgctcg ccgcttctgc aatgttctgc gccagcatat
    71521 atgctggggt ggtgacaatc ttattgtctt catcaaccac gatatcatcg acaggacatg
    71581 gcacatgctc cgcgcccatc tcttccagca cttctgcggt atcgatatca gtaccgatgg
    71641 tcaaacgcag cgggaaatcg aaaattttcg gcagcatcgc cggggcaata cacataaaac
    71701 caagcggttt tccggcctga tgcatcgctt gtgccagcgc ctttaattca cggtcaacgg
    71761 tgcattcgct accaagactg gcaaaattgc ttaaattctt cgccgcgcca aaaccccccg
    71821 gcacaatcaa cgcatccagt tcagcggcat cggcctgggc cagaggacgg atttcaccgc
    71881 gcgttattcg tgccgcttca atcagcacat tgcgcgtttc cgtcatcgct tcgcccgtta
    71941 aatggttgat aacatcaacc tgctgcttat ccggtgcaaa gcagaccgcc tgcgcaccgc
    72001 tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat ttcagaaccg tcatagacgc
    72061 cgcatccgct cagaattacg ccaattttct tcattgtgat catcctttcg caattgactg
    72121 aaacacatta atttttttaa taaaaatggt acgcatcaca catttaactg attcatgtaa
    72181 caaatcattt aagttttgct atcttaactg cgtgcggcct gaaaaacagt gctgtgccct
    72241 tgtaactcat cataataatt tacggcgcag ccaagatttc cctggtgttg gcgcagtatt
    72301 cgcgcacccc ggtctagccg gggtcatttt ttagtggctt ttgccaccca cgctttcagc
    72361 acttctacgt cgtgacgcca ctcttctttc atctcttcaa tccattcacc gacgttatct
    72421 tcccaggcag gaaggtcagg agactgaatt tgctgaccca gctgttgcag atggcgtaac
    72481 cccactgacc ccgccgcacc tttaatttta tgtccttcct caacaatgcc ttttttatcc
    72541 tgcgccgtca gattcgactc cagcacgctg acatagcccg gcatcatctt ctcaaacacc
    72601 gctaacccgt cggtgatcag cttcggtcct acaagtgcga gatactgttc cagcatggga
    72661 atatcgagca atgcttctga tttactgttc tcttctgtcg tcaccgtact ctcctcatca
    72721 tcctgggtat cccagaattt cttgatcatc gcggttagcg ccggaaccga aagcggctta
    72781 ctcagcacat catccattcc agcattgagg tactcttgtt tgtctttcag cacgttagcg
    72841 gttaaggcca ccagcggcgg taaatcctcg cgcggataac gcttcgtcag ttcacgagag
    72901 atatccagcc cggtcatatc tggcaactga atatccagca acaccaggtc gtattcgccc
    72961 ggtttaaaca tctccagcgc cgccttgccg gtcatggcga catcaacgct gttacctaat
    73021 ttttccagca cagaacgcgc aacaatcacg ttcagttcaa tgtcttccac cagcagcaca
    73081 ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat catcgacctc ttctgctacc
    73141 gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct ggtaaccgta
    73201 atatcgccgc ccatattttt cgccagacga cgagaaacgg ccagaccaat accggtgccg
    73261 gtggcaggtt taccgccatg actgtctttc acctggtaat acatggcgaa aattttatcc
    73321 agctcatcct gcggaatgcc gataccagag tcttccactt caaaatgcag catatcgcct
    73381 tcatcgtagc gcacgcgcac ggtaacctgg ccttgctggg tgaatttgac ggcgttactg
    73441 atgagattcc acaggatctg ccgtaaacgc gtcccgtcgg taatgacctg atgcggtaat
    73501 ggcagcgtag gctccaggtt aaagcgcaat cctttttgtt gcgcctgcaa tgcggaaaga
    73561 ttttccagat cggcaaggaa gctggtgaaa tcaaccggct gattatcaag ctggacctta
    73621 cgccgttcca tcttatccat gtcgataata tcgttgaaga tattcccgag cgtgacggcc
    73681 gaaacatgga tggttttgag atatttttcc tgctcggcgg tgagttcggt atccagcaga
    73741 atgcggctca ggccgacgat accattcagc ggtgtacgca attcgtgact gatggtggag
    73801 ataaacgtcg ttttgtcgcg gctggcccgt tcaagcgcat cctgataccg cttacgctcg
    73861 gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc gtagtacggc
    73921 actttacgga tttcaaagca ggctttgcgt ccgtcgggat aatccagcca ctgctcatag
    73981 gtcagtgaca cattatgacg gaacactttt tcatcggttt caatgacttt cgcagcggct
    74041 tccggtgagt aaacatcagc aggtttcaga tgaaccagtt gtttttcgct ttttccggtc
    74101 agcagctcca tcgcgcggtt acagccggaa aactctttat cttcgttacg ataaaaaacc
    74161 aggtcgggtg aagcgtcaag gaaggaacgt aagaatgagg attgctgctc gagctgaatt
    74221 tgtgtctctt cgcgctcttt gatttcaatt ttcagttggc cgaaggtttc ctgcagttct
    74281 gcttccgctt tttcacgaac ggcgatttcc tgatttagct gggcaatatt atcttttaac
    74341 tgaacgttga ggctcaaatc gcgctcgcgc atctcctcca gtttttgcac cagtcgtgac
    74401 agacgttgtc gtgactcctc cagttgctcg acgaccaccg atagaaagta gaccgcccac
    74461 ggcgtaatca gcaaaccaaa gaagatagaa cgaataacat caatgctttc gacctgacca
    74521 tgcagcacca tggttaccgc catttgtacc acaatggcaa gaacgacgag ggccagcgcc
    74581 agtaacattg agaagcgcac cagacctaac ttcatcatca ggtcaacata atactgcgcc
    74641 agcagacgaa tttgcttcat tagggaattc cttcacgaca acctgcccca ataataccca
    74701 attctgacag ttacgttata aattgtgtga gaaatgcgga attcccctca atggcctacg
    74761 ccgttggagg tagccacgga cgtcccagcg ccgatccctg tacgccatgt tcattcagat
    74821 aacgatccag ctcgaccatt cccgtccatc gattttcgca ccacagcgga gcaagcagcg
    74881 tcgggcgacg ggcgctggcg gaaatgcggt ggtagatcac ttccggtggc gtatggcgaa
    74941 tcatttctcc ggcagtgagc gtgtaatcct ccagttcaat tccgttcaaa cgccccgctt
    75001 cccaggcttt cgccataata ctgcctttca caatatgcag cggatgcagc tttatgccgt
    75061 ccacgccggt ttcaaccact cgctccagcg tttgcaaaca ttccgtctgc ccttcgccag
    75121 gcaggccgac aattaagtgg gaacacacct tcagcccgcg ctgacgtgcc agctgggttg
    75181 tacgctgata acaggcaaaa tcatggccgc ggttgatgcg atgcagcgtt ttgtcgtggg
    75241 cggtttgtag ccccagctcc agccacactt cgtaaccctg gtccttatat tcgcaaagca
    75301 gatccagcac cgcatccggc acgcagtccg ggcgggtacc aacacacaaa ccgacaatat
    75361 tggcctggct caccgcctgc tgatacatag aacgcagcac ctgaacttcc gcaaaggtgc
    75421 tggtatacgc ctgaaagtag gccagatagc gtttagcgcg gttaactaaa ttcgcctggt
    75481 gcgccagttg ctcggcaatg gaacgatgct gctgcgcttc atcggcaaac gaggccacat
    75541 tacagaatgt gcagccacca cgcccgatgg taccgtcacg gttagggcag ctaaaaccgc
    75601 cgtgcagcgt cagcttatgc accttttgcc cataacgacg ggtgagatca ccaccaaaca
    75661 tattgactaa tttctgtaac tgcataatct gatagaccgc gccttgaaaa gaggccaaag
    75721 cctgccattt ttagcccaat tcggcgatga cctggatcaa tcgtcccggc ccgcttttat
    75781 caactgcata atcaatcaaa attaccgaaa tttcatgcac aatcacataa atcacttttg
    75841 cttatcttgt gtcagatttt tttatctcct tatggatttt aggcaaaaac agtagcatga
    75901 aacgttatta ccaattaagg cagtataaaa tgctggtttt ctcgtcagtt caaggcagga
    75961 taagggttaa cacgccttta tgacagtcag gaattggctg tttctctaac gacgcccctt
    76021 ttagccttaa atagaaaatc cactttaatt tcagccaatt aataaagaat tttgcgctaa
    76081 agcacatttc tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg
    76141 gaaatccgct ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga
    76201 gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatgcgaggc
    76261 gcgcgtatga cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg
    76321 gaagtggggt tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt
    76381 gagagggata actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag
    76441 gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc
    76501 gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc
    76561 atcgttgcgc aggagcgcgg ctggcgttta gcaaaaaact acgccgtcgg gatgctcttc
    76621 ctgaataaag atcctgaact cgccgctgcc gcacgccgca tcgttgaaga agagctgcaa
    76681 cgcgaaacgt tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt
    76741 gaaatcgccc tctcctctct gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc
    76801 tggcgtccgc gcgatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt
    76861 ctcgaagccg acaaagactt ctacgtctgt agcctgtcga atctggtcaa tatttataaa
    76921 ggtctgtgta tgccggcgga tctgccgcgc ttctacctgg acctcgcgga cctgcgtctg
    76981 gaatcggcta tctgcctgtt tcaccagcgc ttttccacta acaccgtacc gcgctggccg
    77041 ctggcgcagc cgttccgcta cctcgcgcat aacggtgaaa tcaacaccat caccggtaac
    77101 cgccaatggg cgcgtgcgcg tacctataaa ttccagacgc cgctgatccc tgacctgcac
    77161 gacgccgcgc cgttcgtcaa cgaaaccggc tctgactcca gttcgatgga taacatgctg
    77221 gaactcctgc tggcaggcgg gatggatatc atccgtgcca tgcgtctgtt agtgccacca
    77281 gcctggcaga acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc
    77341 atgcatatgg agccgtggga tggtccggca gggatcgtga tgtccgatgg ccgtttcgcc
    77401 gcctgtaacc tcgaccgtaa cggtctgcgt ccggcacgct acgtcatcac caaagataaa
    77461 ctgatcacct gcgcctctga agtcggtatc tgggattatc agcctgacga agtggtcgaa
    77521 aaaggccgcg tagggccagg cgaactgatg gtgatcgaca ctcgcagtgg gcgcattcta
    77581 cactcggcag aaaccgatga tgacctgaaa agccgccatc cctataaaga gtggatggag
    77641 aaaaacgtcc gccgtctggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc
    77701 gaactggacg acgacacgct tgccagctac cagaaacaat ttaactacag cgcggaagag
    77761 ctggattcgg tgatccgcgt actgggtgaa aacggtcagg aagcggtcgg ttcgatgggc
    77821 gacgataccc cattcgccgt gctctccagc cagccgcgca ttatttacga ctacttccgc
    77881 cagcagtttg ctcaggtaac caacccgcca atcgacccgc tgcgcgaagc gcatgttatg
    77941 tcgctcgcca ccagtatcgg tcgtgaaatg aacgtcttct gtgaagcaga aggccaggcg
    78001 caccgtttaa gctttaaatc gccgattctg ctctattccg atttcaaaca gctcacgacg
    78061 atgaaagagg atcactaccg cgcagatacg ctggatatca cctttgacgt cactaaaacc
    78121 acgctcgaag cgacggtgaa agagctgtgc gacaaagccg aaaaaatggt acgtagtggc
    78181 accgtgctgc tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccggct
    78241 ccgatggcgg ttggcgcgat ccagacccgt cttgtcgagc aaagcctgcg ttgcgatgcc
    78301 aacatcatcg tagaaaccgc cagcgcccgt gatccgcacc acttcgccgt gttgctgggt
    78361 ttcggcgcga cggctattta tccatacctt gcctatgaaa cgctgggccg cctggtagac
    78421 acccacgcga ttgccaaaga ttatcgtacc gtgatgctca actatcgtaa cggcatcaac
    78481 aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc
    78541 tcgaaactgt ttgaagcggt cggtctgcac gatgatgtcg tgggcctgtg cttccagggg
    78601 gcggttagcc gcattggggg ggctagcttt gaagacttcc agcaggatct gctgaatctg
    78661 tcgaaacgag cctggctggc gcgtaagcct atcagccagg gcggcctgct gaaatacgtg
    78721 cacggtggcg aataccacgc ctacaatccg gacgtggtgc gcacgctgca acaggcggta
    78781 cagagcggcg agtatagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca
    78841 accacgctac gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgctgat
    78901 gttgaaccgg caagcgaact gtttaaacgc ttcgataccg ccgcgatgtc tatcggcgcg
    78961 ttaagcccgg aagcccacga ggcgctggcg gaagcgatga acagcatcgg cggtaactca
    79021 aactccggcg aaggcggtga agacccggcg cgctatggca ctaacaaagt gtcgcgcatc
    79081 aagcaggtgg cgtccggtcg cttcggcgtt actccggcgt acctggtcaa tgccgacgtc
    79141 attcagataa aagtcgccca gggcgcgaag ccgggtgaag gaggtcagtt accgggtgat
    79201 aaagtcactc cttacattgc caaactgcgc tattcggtgc cgggcgtgac gctgatctcc
    79261 ccgccgccgc accacgatat ctactctatt gaggatttag cgcagctgat tttcgacctc
    79321 aagcaggtta acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgtaggc
    79381 accatcgcga ctggcgtggc aaaagcttat gccgatttaa tcaccatcgc aggctatgac
    79441 ggcggcaccg gcgcaagtcc gctttcatcg gtgaaatacg caggctgtcc gtgggagctg
    79501 gggcttgttg aaacccaaca ggcgctggtt gccaacggct tgcgtcacaa gatccgtttg
    79561 caggtcgatg gcggcctgaa aacgggtgtc gatatcatca aagcagcgat tcttggcgcg
    79621 gaaagttttg gcttcggcac tggcccgatg gtagcgcttg gctgtaaata tctgcgaatt
    79681 tgccacctga acaactgcgc cacgggtgta gcaactcagg atgacaaact gcgtaagaat
    79741 cactatcacg gcctgccgtt caaagtgact aattactttg agtttatcgc ccgtgaaacc
    79801 cgcgagctga tggcacagct gggggtaacg cgtctggtgg atctgattgg tcgcaccgac
    79861 ctgctgaaag agctggacgg tttcaccgcc aaacagcaga aactggcact gtcgaagctg
    79921 ctggagactg ctgagccgca tgctggtaaa gcgctctact gcaccgaaaa caacccgccg
    79981 tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg caaaaccgtt tgtcgatgaa
    80041 cgccagagca aaaccttctg gttcgatatc cgcaacaccg accgttctgt cggcgcatcg
    80101 ctttcaggct atatcgccca gacgcacggc gatcaggggc tggctgccga tcctatcaaa
    80161 gcgtacttca acggcaccgc aggccagagc ttcggcgtgt ggaacgcggg cggcgtggaa
    80221 ctgtacctga ccggtgatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc
    80281 gccattcgtc ctccggttgg ctcagccttc cgcagccatg aagcgagcat tatcggcaac
    80341 acctgcctgt atggcgcgac cggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt
    80401 ttcggcgtgc gtaactccgg tgctatcacc gtggtagaag gcattggcga caacggctgt
    80461 gaatatatga cgggtggtat cgtctgtatt ctgggtaaaa ccggcgttaa cttcggcgca
    80521 ggcatgactg gcggcttcgc ttacgttctc gatgaaagcg gcgatttccg caaacgcgtt
    80581 aacccggaac tggtcgaggt cttaagcgtt gacgatctgg cgatccatga agagcatctg
    80641 cgcggtctta tcaccgagca tgttcagcat accggttctc agcgcggtga agagattctg
    80701 gcgaactggt caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgta
    80761 aaagcactgc tgggtcaccg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa
    80821 ggggtagcaa caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg
    80881 cctaagaaac cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gccgttttcc
    80941 gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag
    81001 tggaaatgcc cggtacacaa ctacatcccg aactggctga agctcgccaa cgaggggcgt
    81061 atttttgaag cggcggaact gtcgcaccag accaacaccc tgccggaagt atgcggacgt
    81121 gtctgcccgc aagaccgtct gtgcgaaggt tcctgcaccc tgaacgatga gtttggcgct
    81181 gtgaccatcg gcaacattga gcgttatatc aacgataaag cgttcgaaat gggctggcgt
    81241 ccggatctgt ccggcgtgaa acagaccggt aaaaaagtgg cgattatcgg cgctggcccg
    81301 gcgggtctgg cgtgtgcgga tgttctgacg cgcaacggcg taaaagcggt cgtcttcgac
    81361 cgtcacccgg aaattggcgg attgctgacc ttcggtattc cggctttcaa gctggaaaaa
    81421 gaggtaatga cgcgccgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat
    81481 accgaagtgg gccgcgacgt gcagctggac gatctgctga gtgattacga tgccgtgttc
    81541 cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc
    81601 gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggtgaa
    81661 acccgcgacg aaccgttcgt cagcatggaa ggcaaacgcg tggtggtcct tggcggtggc
    81721 gacactgcga tggactgcgt gcgcacgtct gtacgccagg gggcaaagca cgttacctgt
    81781 gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg
    81841 gaagaaggcg tagagttcaa attcaacgtc cagccgctgg gtatagaagt gaatggcaac
    81901 ggcaaagtca gcggcgtaaa aatggtgcgc acagaaatgg gcgaaccgga tgccaaaggc
    81961 cgtcgccgcg cggagattgt ggcaggttcc gaacatatcg ttccggcaga tgcggtgatc
    82021 atggcgtttg gtttccgtcc acacaacatg gaatggctgg caaaacacag cgtcgagctg
    82081 gattcgcagg ggcgcattat cgccccggaa gacaacgaca acgctttcca gaccagcaac
    82141 ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg atctggtggt gaccgctatt
    82201 gccgaaggtc gtaaggcggc agacggtatt atgaactggc tggaagttta agcctgtccc
    82261 agcgccgcct cctcagaaaa gagtcggcgc tgaaattctc ctttctttct ggaaagcgcc
    82321 tcgcataatc tgaagaaaac caatgtaata cgctcatctg gattgttacc ggtagatgcc
    82381 cggcgtatag ctgattccat tattcaacgc agcgccatat aaagggacag ataatggaat
    82441 cgctctcaga gagaacctca acaggctacc agcaaatcca cgacggtatt attcacctgg
    82501 tcgatagcgc ccggacggaa acggtacgta gcgttaacgc gctgatgacc gcgacatact
    82561 gggaaattgg ccgacgaatt gtcgaatttg aacaaggtgg cgaggccagg gctgcgtatg
    82621 gtgcgcagct aatcaagcga ctatcaaagg atttaagtct aaggtataag cgtgggttct
    82681 ctgcaaaaaa cttacgccaa atgaggcttt tttacctctt ttttcaacat gttgaaattc
    82741 gccagacagt gtctggcgaa ttaacaccat tgggaattcc gcagacacca tctgcagaat
    82801 tcccatcggc aaagatttgg cagacgctgt ctgccaaatc tttccccctg ccctggtcca
    82861 cctacgtccg tttactttcc gttaaaaacc ctgatgcccg cagcttttat gaaaaagaga
    82921 cgctccgctg tggttggtct gtccgccagc tagagcggca aattacgacc caattttatg
    82981 agcgaacact actgtcacat gacaaatcag ccatgctgca acaacacgct cctgccgaga
    83041 tgcatattct tccgcaacag gcgatacgcg atccctttgt gctcgaattt ctggagttga
    83101 aagatgaata ctcagaatct gattttgagg aggcgctgat caaccacctg atggatttca
    83161 tgctggaact tggggatgat tttgcctttg ttggtcggca gcgaaggtta cgcattgatg
    83221 acaactggtt tcgggtcgat ctgctgtttt tccaccgccg tttacgctgc ctgctaatcg
    83281 tcgatctaaa agtgggcaaa ttcagctata gcgatgccgg acagatgaat atgtatctca
    83341 actacgccaa agagcactgg acactaccgg atgaaaatcc gcccatcggt ctggttctct
    83401 gtgcagagaa aggagccggc gaagcgcatt atgcactggc tggtttgcct aacaccgttc
    83461 tggcgagtga atataagatg caactacctg atgaaaaacg actcgcggat gaactcgttc
    83521 gaacacaggc ggtgttagag gaaggctata gacgccgtta atttcagcgg tttcgaattc
    83581 tccagacact gtctggagaa tctttaaaac gaactaagtt ttaagccatt aaatcagcct
    83641 taaccttcac cacaaccttt ttaatctcac caggttcgcc gacaacgcat cctggtttat
    83701 gcggttctcc cggcataaac acggcgaaca ttcccggttt taagatgatg gcttgctcgt
    83761 tctcaatggc gctgcaaagc tggtaatcat cctcatggtg gaactcttca cactgacgcg
    83821 cagtgcctgc cgtgccaaac agaatccgtt cctcaccgtt taataacagc tggatatcaa
    83881 tgtattgctc gtgcagctcc gcttttttct caacgggcga ttgagtgtta aacgtcatga
    83941 cattcataaa gatattgtcg cactgtaatt cgtaacgacc cggtgctttt tcttgcggcc
    84001 tggcggctaa ggctagtgtt aacgcgtcct gtaacgcagg atgcaatccg gcagacggta
    84061 acgactgtac ttcacccatt atcataattt ttctccctgg gccaacaacg cagccccaag
    84121 taaacctgca tcgtggcggt agtgcgccgc cagtaaatca acatgaaatg ccgctggctc
    84181 ctgcgccaga tacgtttcca ccaacgccag atacccttct gccagaccaa cgctgccacc
    84241 gaccactacg cactggcaat cagttgtggc tttaatatca gcgatcagcc ttgccagcac
    84301 atgtgcggag cagtgaatca gttgctgcgc ctgctcatcg ccctgcccgg cgcgtgtgaa
    84361 aatagttctc gcatccgcgc ccgccagttc accctgcgct gccgctgcaa taccgcgacc
    84421 agaagcaatc gcttccacac aaccagtgcg tccacagccg cagactggac catgaggatc
    84481 agccagcgta tgcccgatgt gccccgccag accgccaggg ccggtgagca gtttgccgcc
    84541 gctcactaca ccgccgccaa caccagtgga aacggtgata aacaccatct cggttatatc
    84601 gccttccagc gcctgatact ccgcccacgc tgcggcctgc gcgtcgttaa tggcaatggt
    84661 cggcaaatcg gtaagttgtt ccaaagtttt gactaacgga aagtgtagca atccaccgag
    84721 attatgcgga ttcagcgcca gcaggctgcc gtcgcgaata attccggttg aagcgatagc
    84781 aacccgctgc gcatgaactt gcaacggaga gactaatgcg gataaggcat catgcaaggc
    84841 ttctggtgtc tggctggctg gcgtaggaag ttcacgacga tcgcggatct gcccatcagc
    84901 gccaatcagc gcggcggcaa gtttagtacc gccgatatca atcgccagtg tggtcatagc
    84961 accgcctttt tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg cgtaatggca
    85021 gaaccgaccg tcaccgccca cgcgccgtgg cgcatcgcat ctgccgcctg agcaggcgtg
    85081 ttgtaacgcc cttcggcaat cacccgacat ccggcgtcgc tcaacgtttt caccagcgcc
    85141 agatccggct cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt gccaataatt
    85201 tcggctccca gcttttggca tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc
    85261 agtaaaccgt gatggtgaat acgtgccagc agcgtttcaa caggcaccgg acgcggtcgg
    85321 tcggtgccgt caatggcgat aatgtccgcg cccgcctgcg ccagcgcatc aacatcttca
    85381 atataggccg tgatgcgtac cggagaatcc tccagatcgc gtttcacaat cccgataatt
    85441 ggcacgctca ccaccgcacg cgtggcttgc agatttgcca caccttcaat gcgaatggca
    85501 accgcgcccg cctgttctgc cgctaatgcc atggcggcga cgatttcggg tttatcgagc
    85561 gggctgtccg gaaccggctg gcaggagaca atcaggccac cgttagcagc gattttttga
    85621 tccagttgtg caagtaacga catacatctt cccttagcga aaggcccggt acagagaccg
    85681 ggcaacagga ttaacttttg gttttgacta aatcgttttt ggcgcggcca aatggcacgg
    85741 caccgctaaa tggtttgccg tcgatagcgt catgagtacg caatgcttcc gggcgcagcc
    85801 aacgctgaac gcgggaaggc atatccagac caatcagcag gatcaccaca aatgtcagac
    85861 tgaacgagag cgatgccagc gcagtaccca gatccagacg ttgagcgatc aacgcgccga
    85921 tgattggggc cagtgcaccg cccaatgcgc cgacgttgta ggtaaagccc aggcccgctg
    85981 cacgctggtc ggtatcgaaa taaccgccaa tcagtttcgg taagatcccg gcgatccctt
    86041 gtccaagcat ttgctggaag aacagtaaca gaccgagcac ccagacgttt gcgccgccaa
    86101 tcgcaaatac cggaataatc agcagctgcg aggccagcag actacaaacg tacgctttgc
    86161 gggttcccag ccagtcaccg aggaagccac cgacgcagca ccccaccgcc gcgccaaagc
    86221 cactaaagaa cagcacattg gcgacagtat gcgggtcata agccagatcg gttttcagat
    86281 acgttggcag cagcgcctga atcggccatg agtagaggaa agcaaacaac acgaccacca
    86341 tcagcattac gcccgttggc cagcgtttgc ccgtactctg caccataaag ctgataaaga
    86401 ttgcggcgca taacagcccg agtacagcga cgattgcggc attttgcagg ttaccggcga
    86461 agcagaacca cagcgcagta gccgccgcca gcgtcattac gatattggca atgcgatgtt
    86521 caccacggta aagaatgtct accatcgtgc gtactggtgc tttacccccg tgtttctctt
    86581 tccagtcttc cgcttccggg atgtttttac gcagccagag agcaaagatg attggcaaaa
    86641 tgccgataaa gaacagcgct ctccagcccc agaccggaac caccagacta tagacctgag
    86701 cggcaacgac ggcccccaca gagaagcctg aaatcaaaaa accactggct ttgttacgca
    86761 gatgttttgg ccagctttca atgacatagg tggcgctgga accgtattca cccgccatcc
    86821 ccatgccgat gaccagacgc gcgataaaca tggtgatgta gcctggcgca aagccgcagg
    86881 ccagcgtccc ggccgagaag agaacgatgc tggtgaccat tgccagacga cgcccgtagc
    86941 ggtcacccat agcgccgagc atcaggccgc cgaaccagcg agagataaag gctgcggaga
    87001 tcagacttgc cgcctgcacc gtcgtcagcc cgaattcacc ctgtacttcg gtgagtacca
    87061 gggcgattaa aacgaaatca aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg
    87121 cgcgccattg tgcacggttg agatggcgat accacgggat attctgggtt gtagtactca
    87181 ttgtgagtct cccgcggtgg gcgatgccca cacgctttgg tatgaaaatt gtagggtaca
    87241 gatgcgttta tttcccctca cccggtaggg gcgagcgagg ggaaaacaac tcacccgcgc
    87301 tcttgcatca actgctgggc cagcgccttc agttctggca gatatttttc atctaccggt
    87361 ccaaacggtt tgcggcacag cggcacagaa acgacatcca tataatgcag gacagttttc
    87421 aggccgcgga atacgcccgt tttgatcagt aaatcaatga ctttgttgca ctcagtttgc
    87481 agtttctgcg cggtctggat atcgccttct ttcagcgcct tcacgatccc ctgatatcgc
    87541 cagcccatga tgttgtaagt actgccgata ccaccatcag cgcctgccag caagccggaa
    87601 gcgaagattt cgtcgtaacc gttatagagc accagatcag gatgttcacg acggatctgt
    87661 tccatctgat agagatcgcc agaagtctgt ttcagcgcac cgacgccagg caacgtaacc
    87721 agggtgttga tctgatccag ggtcagtttt accccactca gggctggaat gttgtacacc
    87781 accatcggca aaccatccgc cgaatcaata attgcccgat agtgatcgca gtgttcttca
    87841 aagctgaaag gatagtagaa cggcgtgacg gcggagacgg catcgaagcc ataacgttta
    87901 gccgatgccg caagttgttg gctttcggcg gtgctgacgc aaccgacgtg ggcgatgagt
    87961 ttaatcttac ctttcgcctc ttcagcgacg atttccagta cctgttcacg ctcggaaagg
    88021 ctttgtacaa aggcttcgcc ggtagaacca cccacgtata aaccgtcgat gccctgctga
    88081 atattgaact gaaccaggcg acgcagactc gctttatcca gtgcttgttg ttgatcaaaa
    88141 ggagtcagga gtgcggccat tacgccacgt aaattcgttg ccataaatac ctctgaagtg
    88201 atgcttatct gataaacgat atacctttat acctgttata ccagatcaat taagcaacac
    88261 cccatacaga aagcttataa tgcgatctgc ttcactaaag tggcattatt tctttttgtt
    88321 ggtggtctga ccgaaggcgt gccaggtagc agagacgctg ttgagatgcg attgcaacgc
    88381 acgatcggct tcatcgggat cgtggcggcg gatcgcgtca acgatcgcaa tatgctgttg
    88441 atagctaacg ttgttatgct cgtgcagagc ctgatcagcc acggttggac gtgcggcgat
    88501 cagccagtcg agcaaggcaa cgtggatcgc catgaagatt gggttaccgg ggatctccgc
    88561 cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat ccagcgactg
    88621 actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg cgtgttcagc
    88681 cgcatagcgc accaggctgg attcaaagaa cagacgtaat tgttcgaaat gggcaatccc
    88741 gccaggatga gaaaggaaat ctttcgccat gccggaaagc tcgccgatga tggtgtccgc
    88801 agaaggacgt gagacgcgag cgcgttcgcc gttgtttatc tgcaccagac ctttgcgttt
    88861 taacgctgcc agcgcttcgc gcaccgaagg acgcccgacg ttaaagaacg ccatcagttc
    88921 gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca tctgttccag
    88981 ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctgc gtaagttgcg
    89041 gccaattgca ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa ggcccattct
    89101 gtaaggtcag tgtgattaac atcatcagtg acatcctatc acaggattga aagtaggggg
    89161 aaatggcggg gttttctctt tgtgcctcat cattactata attaac
//
LOCUS       DAZKJR010000002        79368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKJR010000002 DAZKJR010000000
VERSION     DAZKJR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.002"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            124..384
                     /gene="iraP"
                     /locus_tag="ACHOWS_000093"
     CDS             124..384
                     /gene="iraP"
                     /locus_tag="ACHOWS_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFQ0045747.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            484..1899
                     /gene="phoA"
                     /locus_tag="ACHOWS_000094"
     CDS             484..1899
                     /gene="phoA"
                     /locus_tag="ACHOWS_000094"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFQ0045748.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATVGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            2018..2338
                     /gene="psiF"
                     /locus_tag="ACHOWS_000095"
     CDS             2018..2338
                     /gene="psiF"
                     /locus_tag="ACHOWS_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFQ0045749.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            2440..3555
                     /gene="adrA"
                     /locus_tag="ACHOWS_000096"
                     /gene_synonym="dgcC"
     CDS             2440..3555
                     /gene="adrA"
                     /locus_tag="ACHOWS_000096"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFQ0045750.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3572..4381)
                     /gene="proC"
                     /locus_tag="ACHOWS_000097"
     CDS             complement(3572..4381)
                     /gene="proC"
                     /locus_tag="ACHOWS_000097"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0045751.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            4501..4959
                     /gene="yaiI"
                     /locus_tag="ACHOWS_000098"
     CDS             4501..4959
                     /gene="yaiI"
                     /locus_tag="ACHOWS_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0045752.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5142..5666
                     /gene="aroL"
                     /locus_tag="ACHOWS_000099"
     CDS             5142..5666
                     /gene="aroL"
                     /locus_tag="ACHOWS_000099"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFQ0045753.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            5716..5907
                     /gene="yaiA"
                     /locus_tag="ACHOWS_000100"
     CDS             5716..5907
                     /gene="yaiA"
                     /locus_tag="ACHOWS_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFQ0045754.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            6165..6842
                     /gene="aroM"
                     /locus_tag="ACHOWS_000101"
     CDS             6165..6842
                     /gene="aroM"
                     /locus_tag="ACHOWS_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFQ0045755.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            6914..7198
                     /gene="ppnP"
                     /locus_tag="ACHOWS_000102"
     CDS             6914..7198
                     /gene="ppnP"
                     /locus_tag="ACHOWS_000102"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFQ0045756.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSLFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            7355..7684
                     /locus_tag="ACHOWS_000103"
                     /pseudo
     CDS             7355..7684
                     /locus_tag="ACHOWS_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            7681..7761
                     /locus_tag="ACHOWS_000104"
     CDS             7681..7761
                     /locus_tag="ACHOWS_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0045757.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(7842..8753)
                     /gene="rdgC"
                     /locus_tag="ACHOWS_000105"
     CDS             complement(7842..8753)
                     /gene="rdgC"
                     /locus_tag="ACHOWS_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFQ0045758.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            8878..9786
                     /gene="mak"
                     /locus_tag="ACHOWS_000106"
     CDS             8878..9786
                     /gene="mak"
                     /locus_tag="ACHOWS_000106"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFQ0045759.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(9925..11109)
                     /gene="araJ"
                     /locus_tag="ACHOWS_000107"
     CDS             complement(9925..11109)
                     /gene="araJ"
                     /locus_tag="ACHOWS_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFQ0045760.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(11235..14378)
                     /gene="sbcC"
                     /locus_tag="ACHOWS_000108"
     CDS             complement(11235..14378)
                     /gene="sbcC"
                     /locus_tag="ACHOWS_000108"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFQ0045761.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISSMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIKEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSVREHLRQWQQQLTHA
                     EQKLNALTAITLTLTADEVATALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTMEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            complement(14375..15577)
                     /gene="sbcD"
                     /locus_tag="ACHOWS_000109"
     CDS             complement(14375..15577)
                     /gene="sbcD"
                     /locus_tag="ACHOWS_000109"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFQ0045762.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            15767..16456
                     /gene="phoB"
                     /locus_tag="ACHOWS_000110"
     CDS             15767..16456
                     /gene="phoB"
                     /locus_tag="ACHOWS_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFQ0045763.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            16514..17809
                     /gene="phoR"
                     /locus_tag="ACHOWS_000111"
     CDS             16514..17809
                     /gene="phoR"
                     /locus_tag="ACHOWS_000111"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFQ0045764.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKVTRFSFVIPERLIAKNSD"
     gene            18216..19535
                     /gene="brnQ"
                     /locus_tag="ACHOWS_000112"
     CDS             18216..19535
                     /gene="brnQ"
                     /locus_tag="ACHOWS_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFQ0045765.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            19611..20984
                     /gene="proY"
                     /locus_tag="ACHOWS_000113"
     CDS             19611..20984
                     /gene="proY"
                     /locus_tag="ACHOWS_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFQ0045766.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     SLKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
     gene            21140..22957
                     /gene="malZ"
                     /locus_tag="ACHOWS_000114"
     CDS             21140..22957
                     /gene="malZ"
                     /locus_tag="ACHOWS_000114"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFQ0045767.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSGGLPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLYQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(22962..23543)
                     /gene="acpH"
                     /locus_tag="ACHOWS_000115"
     CDS             complement(22962..23543)
                     /gene="acpH"
                     /locus_tag="ACHOWS_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFQ0045768.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     NSWYDLDAHYDALETCFWQFYPRMMEQASRKAL"
     gene            23636..24706
                     /gene="queA"
                     /locus_tag="ACHOWS_000116"
     CDS             23636..24706
                     /gene="queA"
                     /locus_tag="ACHOWS_000116"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0045769.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDERINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24761..25888
                     /gene="tgt"
                     /locus_tag="ACHOWS_000117"
     CDS             24761..25888
                     /gene="tgt"
                     /locus_tag="ACHOWS_000117"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0045770.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            25911..26243
                     /gene="yajC"
                     /locus_tag="ACHOWS_000118"
     CDS             25911..26243
                     /gene="yajC"
                     /locus_tag="ACHOWS_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0045771.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            26271..28118
                     /gene="secD"
                     /locus_tag="ACHOWS_000119"
     CDS             26271..28118
                     /gene="secD"
                     /locus_tag="ACHOWS_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFQ0045772.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            28129..29100
                     /gene="secF"
                     /locus_tag="ACHOWS_000120"
     CDS             28129..29100
                     /gene="secF"
                     /locus_tag="ACHOWS_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFQ0045773.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            29230..29577
                     /gene="yajD"
                     /locus_tag="ACHOWS_000121"
     CDS             29230..29577
                     /gene="yajD"
                     /locus_tag="ACHOWS_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFQ0045774.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(29615..30499)
                     /gene="tsx"
                     /locus_tag="ACHOWS_000122"
     CDS             complement(29615..30499)
                     /gene="tsx"
                     /locus_tag="ACHOWS_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFQ0045775.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(30798..31337)
                     /gene="yajI"
                     /locus_tag="ACHOWS_000123"
     CDS             complement(30798..31337)
                     /gene="yajI"
                     /locus_tag="ACHOWS_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFQ0045776.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            31488..31937
                     /gene="nrdR"
                     /locus_tag="ACHOWS_000124"
     CDS             31488..31937
                     /gene="nrdR"
                     /locus_tag="ACHOWS_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0045777.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            31941..33044
                     /gene="ribD"
                     /locus_tag="ACHOWS_000125"
     CDS             31941..33044
                     /gene="ribD"
                     /locus_tag="ACHOWS_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFQ0045778.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            33133..33603
                     /gene="ribE"
                     /locus_tag="ACHOWS_000126"
     CDS             33133..33603
                     /gene="ribE"
                     /locus_tag="ACHOWS_000126"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFQ0045779.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            33623..34042
                     /gene="nusB"
                     /locus_tag="ACHOWS_000127"
     CDS             33623..34042
                     /gene="nusB"
                     /locus_tag="ACHOWS_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0045780.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            34120..35097
                     /gene="thiL"
                     /locus_tag="ACHOWS_000128"
     CDS             34120..35097
                     /gene="thiL"
                     /locus_tag="ACHOWS_000128"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFQ0045781.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            35075..35593
                     /gene="pgpA"
                     /locus_tag="ACHOWS_000129"
     CDS             35075..35593
                     /gene="pgpA"
                     /locus_tag="ACHOWS_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0045782.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDHNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(35647..36621)
                     /gene="yajO"
                     /locus_tag="ACHOWS_000130"
     CDS             complement(35647..36621)
                     /gene="yajO"
                     /locus_tag="ACHOWS_000130"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFQ0045783.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(36676..38538)
                     /gene="dxs"
                     /locus_tag="ACHOWS_000131"
     CDS             complement(36676..38538)
                     /gene="dxs"
                     /locus_tag="ACHOWS_000131"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0045784.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEIVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            complement(38563..39462)
                     /gene="ispA"
                     /locus_tag="ACHOWS_000132"
     CDS             complement(38563..39462)
                     /gene="ispA"
                     /locus_tag="ACHOWS_000132"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFQ0045785.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLEALERIHRHKTGALIRAAVRLGALSAGDKGRRALSVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(39462..39704)
                     /gene="xseB"
                     /locus_tag="ACHOWS_000133"
     CDS             complement(39462..39704)
                     /gene="xseB"
                     /locus_tag="ACHOWS_000133"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0045786.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            39910..41358
                     /gene="thiI"
                     /locus_tag="ACHOWS_000134"
     CDS             39910..41358
                     /gene="thiI"
                     /locus_tag="ACHOWS_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFQ0045787.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(41412..42002)
                     /gene="yajL"
                     /locus_tag="ACHOWS_000135"
     CDS             complement(41412..42002)
                     /gene="yajL"
                     /locus_tag="ACHOWS_000135"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFQ0045788.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(41965..42876)
                     /gene="panE"
                     /locus_tag="ACHOWS_000136"
     CDS             complement(41965..42876)
                     /gene="panE"
                     /locus_tag="ACHOWS_000136"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0045789.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGITVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            43044..43535
                     /gene="yajQ"
                     /locus_tag="ACHOWS_000137"
     CDS             43044..43535
                     /gene="yajQ"
                     /locus_tag="ACHOWS_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFQ0045790.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(43663..45027)
                     /gene="yajR"
                     /locus_tag="ACHOWS_000138"
     CDS             complement(43663..45027)
                     /gene="yajR"
                     /locus_tag="ACHOWS_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0045791.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILAATGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADTAANEALEVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(45176..46066)
                     /gene="cyoE"
                     /locus_tag="ACHOWS_000139"
     CDS             complement(45176..46066)
                     /gene="cyoE"
                     /locus_tag="ACHOWS_000139"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFQ0045792.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(46078..46407)
                     /gene="cyoD"
                     /locus_tag="ACHOWS_000140"
     CDS             complement(46078..46407)
                     /gene="cyoD"
                     /locus_tag="ACHOWS_000140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFQ0045793.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(46407..47021)
                     /gene="cyoC"
                     /locus_tag="ACHOWS_000141"
     CDS             complement(46407..47021)
                     /gene="cyoC"
                     /locus_tag="ACHOWS_000141"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFQ0045794.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKVFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(47011..49002)
                     /gene="cyoB"
                     /locus_tag="ACHOWS_000142"
     CDS             complement(47011..49002)
                     /gene="cyoB"
                     /locus_tag="ACHOWS_000142"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFQ0045795.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(49024..49971)
                     /gene="cyoA"
                     /locus_tag="ACHOWS_000143"
     CDS             complement(49024..49971)
                     /gene="cyoA"
                     /locus_tag="ACHOWS_000143"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFQ0045796.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(50431..51906)
                     /gene="ampG"
                     /locus_tag="ACHOWS_000144"
     CDS             complement(50431..51906)
                     /gene="ampG"
                     /locus_tag="ACHOWS_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFQ0045797.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(51950..52528)
                     /gene="yajG"
                     /locus_tag="ACHOWS_000145"
     CDS             complement(51950..52528)
                     /gene="yajG"
                     /locus_tag="ACHOWS_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0045798.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            52833..53150
                     /gene="bolA"
                     /locus_tag="ACHOWS_000146"
     CDS             52833..53150
                     /gene="bolA"
                     /locus_tag="ACHOWS_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFQ0045799.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            53494..54792
                     /gene="tig"
                     /locus_tag="ACHOWS_000147"
     CDS             53494..54792
                     /gene="tig"
                     /locus_tag="ACHOWS_000147"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0045800.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            55038..55661
                     /gene="clpP"
                     /locus_tag="ACHOWS_000148"
     CDS             55038..55661
                     /gene="clpP"
                     /locus_tag="ACHOWS_000148"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0045801.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            55787..57061
                     /gene="clpX"
                     /locus_tag="ACHOWS_000149"
     CDS             55787..57061
                     /gene="clpX"
                     /locus_tag="ACHOWS_000149"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFQ0045802.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            57249..59603
                     /gene="lon"
                     /locus_tag="ACHOWS_000150"
     CDS             57249..59603
                     /gene="lon"
                     /locus_tag="ACHOWS_000150"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFQ0045803.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            59794..60084
                     /gene="hupB"
                     /locus_tag="ACHOWS_000151"
     CDS             59794..60084
                     /gene="hupB"
                     /locus_tag="ACHOWS_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFQ0045804.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            60276..62147
                     /gene="ppiD"
                     /locus_tag="ACHOWS_000152"
     CDS             60276..62147
                     /gene="ppiD"
                     /locus_tag="ACHOWS_000152"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0045805.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVVQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKK
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            62299..62670
                     /gene="ybaV"
                     /locus_tag="ACHOWS_000153"
     CDS             62299..62670
                     /gene="ybaV"
                     /locus_tag="ACHOWS_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0045806.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            62764..63162
                     /gene="fadM"
                     /locus_tag="ACHOWS_000154"
     CDS             62764..63162
                     /gene="fadM"
                     /locus_tag="ACHOWS_000154"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFQ0045807.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(63214..63909)
                     /gene="queC"
                     /locus_tag="ACHOWS_000155"
     CDS             complement(63214..63909)
                     /gene="queC"
                     /locus_tag="ACHOWS_000155"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFQ0045808.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLHHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(63974..65674)
                     /gene="ybaE"
                     /locus_tag="ACHOWS_000156"
     CDS             complement(63974..65674)
                     /gene="ybaE"
                     /locus_tag="ACHOWS_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFQ0045809.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLLLLQLPARDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            65774..66592
                     /gene="cof"
                     /locus_tag="ACHOWS_000157"
     CDS             65774..66592
                     /gene="cof"
                     /locus_tag="ACHOWS_000157"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFQ0045810.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGNVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            66745..67203
                     /gene="decR"
                     /locus_tag="ACHOWS_000158"
     CDS             66745..67203
                     /gene="decR"
                     /locus_tag="ACHOWS_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFQ0045811.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            67233..69005
                     /gene="mdlA"
                     /locus_tag="ACHOWS_000159"
     CDS             67233..69005
                     /gene="mdlA"
                     /locus_tag="ACHOWS_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFQ0045812.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            68998..70779
                     /gene="mdlB"
                     /locus_tag="ACHOWS_000160"
     CDS             68998..70779
                     /gene="mdlB"
                     /locus_tag="ACHOWS_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFQ0045813.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVIMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            70960..71298
                     /gene="glnK"
                     /locus_tag="ACHOWS_000161"
     CDS             70960..71298
                     /gene="glnK"
                     /locus_tag="ACHOWS_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0045814.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            71328..72614
                     /gene="amtB"
                     /locus_tag="ACHOWS_000162"
     CDS             71328..72614
                     /gene="amtB"
                     /locus_tag="ACHOWS_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFQ0045815.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(72663..73523)
                     /gene="tesB"
                     /locus_tag="ACHOWS_000163"
     CDS             complement(72663..73523)
                     /gene="tesB"
                     /locus_tag="ACHOWS_000163"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFQ0045816.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            73741..74310
                     /gene="ybaY"
                     /locus_tag="ACHOWS_000164"
     CDS             73741..74310
                     /gene="ybaY"
                     /locus_tag="ACHOWS_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFQ0045817.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQPAI
                     QQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVL
                     PFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQ
                     ASGGATATVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(74341..74652)
                     /locus_tag="ACHOWS_000165"
     CDS             complement(74341..74652)
                     /locus_tag="ACHOWS_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFQ0045818.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            74815..74911
                     /gene="ffs"
                     /locus_tag="ACHOWS_000166"
     ncRNA           74815..74911
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOWS_000166"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            75031..75384
                     /gene="ybaA"
                     /locus_tag="ACHOWS_000167"
     CDS             75031..75384
                     /gene="ybaA"
                     /locus_tag="ACHOWS_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFQ0045819.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(75426..76976)
                     /gene="pdeB"
                     /locus_tag="ACHOWS_000168"
     CDS             complement(75426..76976)
                     /gene="pdeB"
                     /locus_tag="ACHOWS_000168"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFQ0045820.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWSSTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(77140..77610)
                     /gene="ylaC"
                     /locus_tag="ACHOWS_000169"
     CDS             complement(77140..77610)
                     /gene="ylaC"
                     /locus_tag="ACHOWS_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFQ0045821.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(77726..78277)
                     /gene="maa"
                     /locus_tag="ACHOWS_000170"
     CDS             complement(77726..78277)
                     /gene="maa"
                     /locus_tag="ACHOWS_000170"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFQ0045822.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(78450..78668)
                     /gene="hha"
                     /locus_tag="ACHOWS_000171"
     CDS             complement(78450..78668)
                     /gene="hha"
                     /locus_tag="ACHOWS_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFQ0045823.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(78694..79068)
                     /gene="tomB"
                     /locus_tag="ACHOWS_000172"
     CDS             complement(78694..79068)
                     /gene="tomB"
                     /locus_tag="ACHOWS_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFQ0045824.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        19241 a        19687 c        21265 g        19175 t
ORIGIN      
        1 gtaatcaaac aaaaaatatt tgcgcaaagt atttcctttg tcataaaaat aatacttcca
       61 gacactatga agttgtgaaa cataatgtta acttctccat actttggata aggaaataca
      121 gacatgaaaa atctcattgc tgagttgtta tttaagcttg cccaaaaaga agaagagtcg
      181 aaagaactgt gcgcgcaggt agaagctttg gagattatcg tcactgcaat gcttcgcaat
      241 atggcgcaaa atgaccaaca gaggttgatt gatcaggtag agggggcgct gtacgaggta
      301 aagcccgatg ccagcattcc tgacgacgat acggagctgc tgcgcaatta tgtaaagaag
      361 ttattgaagc atcctcgtca gtaaaaagtt aatcttttca acagctgtca taaagctgtc
      421 acggccgaga cttatagtcg ctttgttttt atttttaatg tatttgtaca tggagaaaat
      481 aaagtgaaac aaagcactat tgcactggca ctcttaccgt tactgttttc ccctgtgaca
      541 aaagcccgga caccagaaat gcctgttctg gaaaaccggg ctgctcaggg cgatattact
      601 gcacccggcg gtgctcgccg tttaacgggg gatcagaccg ccgctctgcg tgattctctt
      661 agcgataaac ccgccaaaaa tattattttg ctgattggcg atgggatggg ggactcggaa
      721 attactgctg cacgcaatta tgccgaaggt gcgggcggct tttttaaagg tatcgatgca
      781 ttaccgctta ccgggcaata cactcactat gcgctgaata aaaaaaccgg taaaccggac
      841 tacgtcaccg actcggctgc atcagcaacc gcctggtcaa ctggtgtcaa aacctataac
      901 ggtgcgctgg gcgtcgatat tcacgaaaaa gatcacccaa cgattctgga aatggcaaaa
      961 gccgcaggtc tggcgaccgg taacgtttct accgcagagt tgcaggatgc cacgcccgcc
     1021 gcgctggtgg cgcatgtgac ctcgcgcaaa tgctacggtc cgagcgcgac cagtgaaaaa
     1081 tgtccgggta acgctctgga aaaaggcgga aaaggatcga ttaccgaaca gctgcttaac
     1141 gctcgtgccg atgttacgct tggcggcggc gcaaaaacct ttgctgaaac ggcaaccgtc
     1201 ggcgaatggc agggaaaaac gctgcgtgaa caggcacagg cgcgtggtta tcagttggtg
     1261 agtgatgctg cctcgctaaa tgcggtgacg gaagcgaatc agcaaaaacc cctgcttggc
     1321 ctgtttgctg acggcaatat gccagtgcgc tggctaggac cgaaagcaac gtaccacggc
     1381 aatatcgata agcccgcagt tacctgtacg cctaatccgc aacgtaatga cagtgtaccg
     1441 accctggcgc agatgaccga caaagccatt gaattgttga gtaaaaatga gaaaggtttt
     1501 ttcctgcaag ttgaaggtgc atccatcgat aaacaggatc acgctgcgaa tccttgtggg
     1561 caaattggcg agacggtcga tctcgatgaa gccgtacaac gtgcgctgga attcgctaaa
     1621 aaggatggca acacgctggt catagtcacc gctgatcacg cccacgccag ccagattgtc
     1681 gcgccggaca ccaaagcgcc gggcctcacc caggcgctaa ataccaaaga tggcgcagtg
     1741 atggtgatga gttacgggaa ctccgaagag gattcacaag aacataccgg cagtcagttg
     1801 cgtattgcag cgtatggccc acatgccgcc aatgtcgttg gactgaccga ccagaccgat
     1861 ctcttctaca ccatgaaagc cgccctgggg ctgaaataaa accgcgcccg gcggtgaatt
     1921 ttcgctgtcg ggtggttttt ttgctgttag caaccagact taatggcaga tcacgggcgc
     1981 aaacgctcat ggttaaaaca tgaagaggga tggtgcgatg aaaataacat tactggttac
     2041 cttgcttttc ggactggttt ttttaaccac cgtcggcgct gccgagagaa ccttaacccc
     2101 gcaacaacag cgtatgacct cctgtaatca gcaggcgacg gcgcaggcgt tgaaagggga
     2161 tgctcgtaag acctacatga gtgattgcct gaagaacagc aagtctgcgc ctggtgaaaa
     2221 aagtttgacg ccacagcagc aaaagatgcg cgaatgtaat aatcaagcaa cgcaacaatc
     2281 tctgaaaggg gatgatcgta ataagtttat gagcgcctgc ctcaagaaag ccgcctgata
     2341 cctgatagtg ctaacgggtg agctacgaaa atggctcacc cgaaatttca tacttctgcc
     2401 tttagccccg tctctataat ttgggaaaat tgtttctgaa tgttcccaaa aataatgaat
     2461 gatgaaaact ttttcaaaaa agcggcggcg catggggagg aacctccttt aactcctcaa
     2521 aacgaacatc agcggtccgg gctgcgcttt gcccgtcgcg tcagactacc ccgcgcggtt
     2581 ggcctggctg gcatgttctt accgattgct tcaacgctgg tttcgcatcc gtcgccgggc
     2641 tggtggtggc tgttgttggt cggctgggcg ttcgtctggc cgcatttagc ctggcaaata
     2701 gcgagcaggg ccgtcgatcc gcttagccgg gaaatttaca acttaaaaac cgatgcagta
     2761 ttagcgggaa tgtgggtagg cgtaatgggc gtaaacgcac tgccttccac cgcgatgttg
     2821 atgattatgt gtctgaattt gatgggggca ggcggccccc gtctgtttgt cgcgggtctg
     2881 gtgttgatgg tggtctcctg ccttgtcacc ctcgagctga cgggcatcac cgtgtcgttc
     2941 aatagcgcac cgctggaatg gtggctctcc cttcccatta ttgtcattta tcctctgctg
     3001 tttggctggg tcagctacca gacggcgact aaactggcgg aacataaacg caggttgcag
     3061 gtcatgagta cccgcgacgg tatgacgggc gtgtataacc gacgtcattg ggaaactatg
     3121 ttacgcaatg aatttgataa ctgtcggcgg cataatcgcg atgcaacgtt actgattatc
     3181 gatatcgacc atttcaagag catcaacgat acctggggcc atgatgtggg tgatgaagcg
     3241 attgtggcgc ttacccgaca gttacaaatt accctacgcg gtagcgatgt gattggtcgg
     3301 tttggcggcg atgagtttgc agtaatcatg tccggtacgc cagcagagag cgccatcacc
     3361 gccatgttac gggtgcatga agggctaaac acgttacgct tgccgaatac gccacaggta
     3421 actttacgga ttagtgtggg ggttgcgccg ctgaatccac aaatgagtca ctatcgcgag
     3481 tggttgaaat cggcagattt ggcgctctac aaagcaaaga aagccggacg taaccgcacc
     3541 gaagtggcgg cctgacgtcc ggcaaaagcc atcaggattt gctgagtttt tctgattttt
     3601 ccatacactt cgtcatcgct tcgatcactg cggcacggaa gcctttctct tccagtacgc
     3661 gtaccgcctc aatggtggtg cctcccggtg agcagaccat atctttcagt gttcccggat
     3721 gttctcccgt ttccagcacc atttttgcgg aacccatgac cgcctgagcg gcaaatttat
     3781 acgcctgggc gcgtggcatc ccgcccagca cggcggcgtc ggccatcgct tcgataaaca
     3841 taaagacgta ggctggcgaa gaaccgctca caccgaccac cgggtggatc atcggctcag
     3901 caatcacttc cgcttcgcca aagcagcgga aaatattcag cacatcagcg gtatcttctg
     3961 gggttaccag cgcgtttggc gttacggagg tcatcccggc attaaccagc gcgggagtgt
     4021 tcggcatggc gcggataatt ttccggtcat ggcccagcgc gcgggcaagc tggtcgagcg
     4081 tgatacctgc agcaatagaa acgaccagag agtctttatt caggctggag gtgatttcgc
     4141 taagcacttt aatcatgatg cctggtttaa cggcggcaaa aatgatgtcg gcgatttgcg
     4201 ctacttcttg cgccgattct gcggcgttga tgccgaactg gtcatgcagg gcggtgactt
     4261 tatccgggga gggggtatat acccagattt gccctggaag cacctgaccg ctggcaatca
     4321 gaccgccgag aatggctttt cccatattgc cgcagccaat aaaaccgatt ttcttttcca
     4381 ttgcctcact cctgccgtga aattcattgt tttgataatc gctggcagaa gcataaacag
     4441 aactatgccg gaaggcaaaa gcgcgacaca atagaggatt acccaacaaa ggatgacttt
     4501 atgacaattt gggtggatgc cgacgcgtgt cccaatgtaa ttaaagagat tttgtatcgc
     4561 gcggcggaac gtatgcagat gccgctggta ctggtagcaa accagagttt acgcgtgccg
     4621 ccatcgcgat ttattcgtac gctgcgcgtc gcggcaggtt tcgacgttgc tgataacgaa
     4681 attgttcggc agtgtgaagc gggcgatttg gtgatcaccg cagatatacc tttggctgct
     4741 gaagccatcg agaaaggcgc ggcggcgctt aatccgcgcg gcgaacgtta cacgccagcg
     4801 accattcgtg agcgcctgac gatgcgcgat tttatggata ccttacgtgc cagtgggatc
     4861 cagaccggag gaccagatag tctttcacaa cgtgaccgcc aggcttttgc tgcggagctg
     4921 gagaagtggt ggctggaagt gcaacgtagt cgtggctaaa tgtaatttat tatttacact
     4981 tcattcttga atatttattg gtatagtaag ggatgtattg agattttcac tttaagtgga
     5041 attttttctt tacaatcgaa attgtactag tttgatggta tgatcgctat tctcatgaca
     5101 ccggctttcg ccgcattgcg acctattggg gaaaacccac gatgacacaa cctctttttc
     5161 tgatcgggcc tcggggctgt ggtaaaacaa cggtcggaat ggcccttgcc gattcgctta
     5221 accgtcggtt tgtcgatacc gaccagtggt tgcaatcaca gctcaatatg acggtcgcgg
     5281 agatcgtcga aagggaagag tgggcgggat tccgcgccag agaaacggcg gcgctggaag
     5341 cggtaactgc gccatccacc gttatcgcta caggcggcgg cattattctg acggaattta
     5401 atcgtcactt catgcaaaat aacgggatcg tggtttattt gtgtgcgcca gtatcagtcc
     5461 tggttaaccg actgcaagct gcaccggaag aagatttacg gccaacctta acgggaaaac
     5521 cactgagcga agaagttcag gaagtgctgg aagaacgcga tgcgctatat cgcgaagttg
     5581 cgcatattat catcgacgca acaaacgaac ccagccaggt gatttctgaa attcgcagcg
     5641 ctctggcaca gacgatcaat tgttgatttt cgagcgccta tacttaacgt tcatcccgtg
     5701 aaataaggaa gaacgatgcc aacgaaaccg ccttatcctc gtgaagcata tatagtgacg
     5761 attgaaaaag gaaagccagg acagacggta acctggtacc aactcagagc cgatcatcct
     5821 aaaccagact cgttgatcag tgaacatccg accgctcagg aagcgatgga tgcgaaaaaa
     5881 cgctatgagg accctgacaa agagtgaccg catcagactg ctcggaaggg attctgagtg
     5941 ccgccacaag ggatctgcgt cacatttttc acaattcatg tttttcaaat aattagaata
     6001 ttaaacaata acaatccatt actggaatca tttggaatct ttacattatg cagtgcaggt
     6061 ctgctgctac gctttttgtc atttgtagca caagtaagtg tcagcagtgg tgcttaacac
     6121 ttgcccggta attaacgacg aaataaaagt aaggtggaag aacaatgagt gcgtcgttgg
     6181 cgatcctgac catcggcatt gtacctatgc aggaagtttt gccgctcctg acagaataca
     6241 ttgacgaaga taatatttcc catcatagcc tgctggggaa gttaagccgc gaagaagtga
     6301 tggcggagta cgcgccagaa gcaggcgaag acaccattct cacattatta aatgacaacc
     6361 atctggccca tgtttcgcgt cgcaaagtgg agcgagacct gcaaggtgtg gttgaagtgc
     6421 tcgataatca gggttacgac gtcattatat taatgagtac agcaaacatt agtagtatga
     6481 ccgcgcgtaa tacgatcttt cttgagccgt cgcggatatt gccaccgctg gtttcctcta
     6541 ttgttgaaga tcatcaggtg ggggttatcg taccggttga ggagttgctg accgttcagg
     6601 cgcaaaaatg gcaaattttg cagaaaccgc cagtattttc attgggtaat cccattcatg
     6661 attcggaaca aaaaatcatt gatgccggga aagaattact ggcaaaaggt gcagatgtca
     6721 tcatgctgga ttgtttggga tttaaccagc gtcatcgcga tttactgcaa aaacagctcg
     6781 atgttcctgt cttgctctct aacgtattga ttgcacggct ggctgcggaa ttactgatgt
     6841 aattttgcgt gacagccagc gcctctggcc cctatagtga agtagatgtt caactaccag
     6901 acagggccag tttatgcttc aaagtaatga gtacttttcc ggcaaagtga aatcgatcgg
     6961 cttttccagc agcagcactg gtcgcgccag cgtgggtgtt atggttgaag gtgaatacac
     7021 cttcagcacc gctgagcctg aagagatgac ggtaatcagt ggcgcgctga atgtgttact
     7081 gcctgacgcg accgactggc aagtgtatga agccggttcg ctgtttaatg ttcccggtca
     7141 cagcgagttt catctgcaag ttgccgaacc cacctcttat ctgtgccgct atctgtaatt
     7201 cctcgccttc cccttgaacg ggagggcatt tttctgaaat gtcctctctt taacccataa
     7261 taatatttcc tttgctggga ttttttcgat ttccgatata ttcataattc atcaaggttg
     7321 atataaatat cagcgaagat ctccagatac tattgcggaa ctggcgacgt taaaagataa
     7381 atcagatgat gaatggtggt gtgcattgct gcaaagtcgc aatgatcatt tgcgccaaac
     7441 ggcattgcac aacgcacata ctccggtgtt gttaacgaca ttgactgagc ctcaggagcg
     7501 gtcgctggct attaataatt cacagctggc tgccgatgtg aaaacggcgt ggttaaaaga
     7561 ggagccatca ttactcttat ttgtcgaaca acccgatctt tcgctgttac gtgatttagt
     7621 gaaaaacggg gcaacgcgaa aaattcgcag tgaagcgcgt caccggcttg aggaaaaaca
     7681 atgacacaac gcccctggag caagttacag cataaaacgc ataatattgc tgcgctaaga
     7741 attattgccc gctgtagcta acgattatat tcctggtgtg gcttcgtacg ccggataaga
     7801 cgcggcaggc gtcgcatccg gcattaaagg taaatcagca attaacgttg tgcttcgcca
     7861 cctaatcctt caatcaggtt ttgaattaac gctgccagtt caccagtcat caggatgaaa
     7921 tcggcatcaa agcgctgggc gaaatcttca cggtcgatat cttcgttttg atcgcgcagc
     7981 tcgtcgcaga acttcagacg cttgagcgaa ccatcgtcgc acatcacaaa ctgaatgcgc
     8041 tgctgccagt cgagcgccag tttagtcacc acttttccgg cttcaatgtg gttggtgatc
     8101 tcttcgctgg tcagatcttg tttcttcgcg cggatcactc cgccatcttc cagcaacgat
     8161 ttcagctcgg cttcatcaag cagctggaag ccctgtgccg cgctgccgga gcgaacccat
     8221 tcggtcagcg tcagttcaat cgggttttcc atgctcaatg gtacaaccgg taacgacccc
     8281 aggcttttac gcagcagtgc cagcgtatct tccgcttttt tggcactggc gcagtccacc
     8341 ataatcaaac cgttaaccgt gtcgatccac atcattgtct ggctaaaacg gctgaaagca
     8401 cgcggtagca gagagtgcag cacttcgtct ttcagcgaat ctttttcggt tttcttcagc
     8461 ttacgcgcct gttccgcttc cagtttggcg attttcgctt ccagcgcctg tttaatcacc
     8521 ggagacggga ggattttttc ttctttgcgc gcgcagataa caatttgacc attggcaacg
     8581 tgcgttaacg catcgctgtg cgatcccatc ggaggaaccc agcccatctt cgccatgtcc
     8641 tggctgccgc atggggtaaa tgccatcgag gctagctgtt tttccatctc ttctgcacgc
     8701 aacgaaatct cgcggctaag acggtaaacc attaaatttt tgaaccacag catgataatt
     8761 tccacggcct tgtcgttaaa tttagcgggc atgataacga attgtcggcg gccttgcatt
     8821 gccaatccag ttgtccgtct ctacgctatt gatattgaaa aaaataagga gagtaccgtg
     8881 cgtataggta tcgatttagg cggcaccaaa actgaagtga ttgcactggg cgatgcaggg
     8941 gagcagttgt accgccatcg tctgcccacg ccgcgtgatg attatcggca gactattgaa
     9001 acgatcgcca cgttggttga tatggcggag caggcaacag ggcagcgcgg aacggtaggt
     9061 atgggcattc ctggttcgat ttcgccctac accggcgtgg tgaagaatgc caattcaacc
     9121 tggctcaacg gtcagccatt cgataaagac ttaagcgcga ggttgcagcg ggaagtgcgg
     9181 ctggcaaatg acgccaactg tctggcggtt tcagaagcag tagatggcgc ggcagcggga
     9241 gcgcagacgg tatttgccgt gattatcggc acgggatgcg gcgcgggcgt ggcattcaat
     9301 gggcgggcgc atatcggcgg caatggcacg gcaggtgagt ggggacacaa tccgctaccg
     9361 tggatggacg aagacgaact gcgttatcgc gaggaagtcc cttgttattg cggtaaacaa
     9421 ggttgtattg aaacctttat ttcgggcacg ggatttgcga cggattatcg tcgtttgagc
     9481 ggacatgcgc tgaaaggcag tgaaattatc cgcctggttg aagaaagcga tccggtagcg
     9541 gaactggcat tgcgtcgcta cgagctgcgg ctggcaaaat cgctggcaca tgtcgtgaat
     9601 attctcgatc cggatgtgat tgtcctgggg ggcgggatga gcaatgtaga ccgtttatat
     9661 caaacggttc cgcagttgat taaacaattt gtcttcggcg gcgaatgtga aacgccggtg
     9721 cgtaaggcga agcacggtga ttccagcggc gtacgcggcg ctgcgtggtt atggccacaa
     9781 gagtaaaaaa accaggcaat tagcgcatca tgcctgatgc gacgcttgcc gcgtcttatc
     9841 aggcctacaa aatgagcagc ccgtaggccg gataaggcgt ttacgccgca tccggcaata
     9901 agcaacgcgc acggtgacag taatctaccc cagtggtttc gccagcaccg gactatccgc
     9961 cgcttgctgg cgcttatagc gaccatacag cagcaacgac gacatcgcag caaacgaaag
    10021 cagggcggca ggcagcgcca cgtaattata tgccagcccc agcgtcagca tcataccacc
    10081 gcaatatgcg ccgacggcgc taccgaggtt aaacgctatt tgcccacctg cggcacctaa
    10141 taactctccg cctttggcat tttgcagtaa caatatttgc agtggtgctg aaagggcaaa
    10201 taatcccgcg caacaaataa atgcaaaaat aagcgacgtt gttttcatgc cgccgaagaa
    10261 aaagagcatc agcagtgcca gcacaataat aaagtcagtt actgccgcaa tgcgcagtgg
    10321 tgaataacgt cctgaaatcc ggccacttag cacattcccc agcaccatcc ctagcccaac
    10381 taacatcata ataaaagtca tcgccgtttc cgaaaaaccg gaaataaaca tcatgtatgg
    10441 ctttacgtag ctgaaccagg caaatacacc tgcgttgcca aacatggtgg cggcgaaaat
    10501 taaccacggg gccgggctgc gtaaaaagtg aaattgttcg cgcagctttc ctttcgcttc
    10561 gtcgcgaata tccggcaccc aaaaatagac cgatgccatc accgcaatat taaaaacagc
    10621 gatcaataaa aaggtgtagc gccagctaaa ttcctgactt aaatacgttc ccagcggaat
    10681 gcccagcaaa ttggcgactg tcatcccgga aaccatcccc gccacggcgg cggtgacttt
    10741 tccgggtttg ataatttttg ataacacgat cgcgccgacg ccaaaaaatg cgccatgcgg
    10801 aaagccggat accagccgac caatggcgag catcaggtaa gacgaagaga gcgtgaacat
    10861 ggcgttgcca atgacgcaca acgccaccag aaacaataag atatgtttga gtgagtagcg
    10921 gctggaaaag agtgcgatga ttggcgcacc gaccaccacc cccagcgcat aatacgagat
    10981 catatgcccg gcagcaggaa tcgaaattcc tacgttatgc gccagctccg tgagcacgcc
    11041 cataatgcca aattcggcca tccccaaacc aaacgtgccc agagccaaag ataaaatgac
    11101 ttttttcata ccaccatcac aaccaccaga acggctacca atcaccgtag ccaataaagt
    11161 gatagcttgc aggataacga ccagcaaggc caagagataa ttcgcccctc tgtattcatt
    11221 atcctgctga atagttattt cactgcaaac gcactttcca gtttgctgta gcccaggccg
    11281 ttgatcttct tcactttgat ctgcaccgga atacgctctt tcatcgcttc aacgtggcta
    11341 atcacaccga tggttttgcc actggcattc agggcatcca gcgcatcaag ggcggtatcc
    11401 agcgtttcgc tatccagcgt gccaaaacct tcatcaagga acagcgagtc aatacgcgtt
    11461 ttatggctga ccagatccga aagcgccagc gccagcgcca gactgacgag gaaactttcg
    11521 ccgccggaaa gggtacgggt atcgcgtacc gcatctgcct gccaggtatc aacaacctcg
    11581 acttccagcg cctcgctggc tttgcgctgt aacagatagc gcccgtgcag ccgggtaagt
    11641 tgctgattag cgagatggac taaattatcc agcgtcagcc cctgggcaaa cttgcggaat
    11701 ttatcgccct ctttggaacc tattagcgaa ttcagatatc cccagtcctc aacctgctgc
    11761 gtcatttgag caatttgctg cattaaggtt tgttgttgct gacggttgtc tgcatcctgc
    11821 ttcagctgct ggcgaatctc gccctgactg gtggtgtttt cacgcaactt ttggtgagtt
    11881 tgcgctaact cttgctgaat ctgctccatc gtcacagtga gagccaaccc gtcaggtcgg
    11941 tgttgctgat gctgtgccag cgtttctgct gtctgagtga ccagcgtttg cgcctgacgg
    12001 cgctggtttt ccagattctg cttgagctgt tccagttgcg ttagtgtttg ttcatccatt
    12061 agcgccgcaa ggaacgcctg ctgatcgtca aagacgctgg cctgtagcgc ggtgtcaaac
    12121 tgcgcctggg ctttttgcag actttgcgcc gccagaacgt cctgttgctg taacgtctgc
    12181 tgctggctgt gtaatgcgag acattgttca tgaacctgcc gccagttttc cagcaccaca
    12241 gtttcttcgc tgtgcgggag ttcatcactt tgcggcaacg tttccagaat cggcgtcagc
    12301 tgctgcatac ggttttgcag cgcggttaat tcgttctggc gatgctgcca gctctgcgct
    12361 tcttgctgac gcgtcgccaa ccagctctct tcttcatctt cctgtggcaa tgtcagtgca
    12421 taacccgcca atgccgttaa gagctgctgc tggcgttgtt caatttgctg ttgatactgg
    12481 ataatttgct gattatgcgc ggcaatctgc ccttgtaatt catgtcgttg gctgagtaac
    12541 cgcagctggc gttcgtgctc atcttgtgca tccagccacg gttgaatatc gtcctgtggc
    12601 tgcaaggtga tattgaggct ggccgtgacg gcttgccatt gttgagtaag tgcttgctca
    12661 tcttgtcgga ggctttgcgc ttcgttttca tcgcgctgaa gttgctttgt taaggcgtcc
    12721 agttgcccac gtagcgtcgc accttcttca ccgagctttt taacttcgtt ttccagcgcc
    12781 agtaatcgag actgattaac gccaggctcc agcgcctgat acgcctcgac cgccgggtgg
    12841 ctggtggaac cacaaagtgg acaaggctga cccgcctgta actgtgcacg ttgagcttcc
    12901 agcgttttga tgcgcgcttc ctgctcgcaa atagttttca catcggcaag ttgctgcgtc
    12961 ttttctttat aacgctggcg catttcgtta agtgcggcgt tacgttgcgt ctgttcttgc
    13021 gtgacattct ggatagcgac ctgtaattgc gccaggcgtt tttgttgggg aacaatctgt
    13081 ccatgcagcg cgaccaggcg ctgacgcagc gtgcgttgct cagcatgttg cgccagggcg
    13141 gtagcaactt catcggcggt taacgtcaac gtgatcgccg taagcgcatt aagtttttgc
    13201 tcagcatggg ttaactgttg ctgccattgc cgcagatgct cgcgaacgct ggtttgttgg
    13261 gagaactgcg cacgccaacc cgccagttcg ttgttccact gacggaagcg gtcgtgttcc
    13321 tgtaaccagg cattcaggct ttgttgctgc tgctgtaatt ctgctgactg cttcgccgcg
    13381 tggtggcgaa tgctcgcccg aagcgccatt gtgctctgta agcgagtatt tacttcttta
    13441 atctgctggc gagtatgcgc cagcgccgcg ctgtgttctg cgatgcgttc ccagtgcgga
    13501 cgaagatttc gtgccggttg tgccagactg agcgccgcca gttgaggttg cgctttttct
    13561 tcttcggcta acgcctgttg caaggcctgc tgacggcggc tggcttcttg ctgcaattcg
    13621 tccagacgcg ttaaccagtt tagtgattgt tgttcttgct gctgcgcggt aagtaactgt
    13681 ttttcttcgt cagtaagtac ctgcaaactc gctgtcagcg attgcacttg ttccggcgtg
    13741 agcaacgcga cgccgctggc ctgcgcttgc agcttctcta gctcagtgcg ggccgatttg
    13801 tgctgctcaa acaccatcga cgagatttgt ccgtagattt cagtgccggt taactcctcg
    13861 agcaattccg cgcgttcttt gggtttggca ttcaggaagg cagcaaattg cccctgcgaa
    13921 agcagcatcg aacgggtgaa gcgcccgtaa tccagtccgg ttaacgtcgc cgtcagttcc
    13981 agcttatctt tcactttgtc ggcgagaatt ttgccatcgg cgcagcgcgc cagctctacg
    14041 cgtggcacct gcaaattacc gtcgggctgg ttacgcgccc gattctggct ccagaacgcg
    14101 cgatacgctt cacctttcac ttcaaactcc acctccgcca gacattcggc ggtatcgcgg
    14161 gtcatgagat cattttgcga ttgtgaaacg ttagaaaggc gcggagtttc gtgatacagc
    14221 gccagacaaa tggcgtccag cagggtggtt ttccccgcac cggttgggcc ggtaatggca
    14281 aacagcccgt tgctggcgaa cggctcgcgg gtgaaatcaa tcttccattc gccttttaat
    14341 gagttcaggt ttttcaggcg caggctgaga attttcatgc ttcgtgttct ccggcgaggc
    14401 tatgcaacgt cgtggcgaaa agatgctgca gacgttgctg ctgcgattcc tccagttctt
    14461 ccagtgccag acggcgattg aacacctctt cgacgctgag ttcgctgagg gtttcacgct
    14521 gttggctggc taacacacgc tcgcgctgtt cacgactccg gcgtaccagc aatacttcga
    14581 caggcaatga ttcggttaat gcctggattt tgcgctgaat atcatgcaga tactcatcag
    14641 tagtgatttc gatatccagc cagacaggtg gctcctgtga tacatcgcgc cactgttcca
    14701 gctgttcggt aatcgacgcc agatcgcctt tcagcaccgc catgggttgc gttaccggta
    14761 cgttcaggtt ttccacgctc tctaatttgc cgtttgaaaa tgtcacaaga tggacatatt
    14821 tactcttacc gcattcatca aaactcagtg gaatggggga accgcaatat cgaacatgtt
    14881 ccatgccgcc aataatctgt gcgcggtgaa tatgcccgag cgcgatgtag tcggctggtg
    14941 gaaagttttg tgccggaaac gcgtccagcg tgccaatata gatgtcacgc acggcatcac
    15001 ttttactggc acccacggtc gttaaatgtc ccgtggcgat gatgggcaga ggctgatcgc
    15061 cgcgcagttt gcaggcatcg gcatagtgtt gttgataata atcagtaata gccgccagca
    15121 aatgctgctg tttttcaata ccgttaagcc ccgcttggct ggtaataatg tcacgtgggc
    15181 gtaaaaacgg aatggggcac agtactgcgc ctggtgtccc gtcgcgacga ggcaagattt
    15241 gcggcgcatg tccggcgctg gcgaccacgg ttgtattgag gaacgccatg atatcgcgcg
    15301 attcattcag cgtggcgacc gagtcatggt ttcctgccag taccaccaga tgacagccag
    15361 tttgctgtaa attgacaaca aaacggttgt ataacgtgcg ggcgtaactg ggcggcgagc
    15421 cggtatcgaa aacatcaccg gcaacaataa tcgcatccac ctgatgggct tgtgctgtct
    15481 ccagcagcca gtcaagaaaa gcctgatgtt cggcttcgcg gcttttactg tagaagttct
    15541 ggccgagatg ccagtctgag gtgtgaagga tgcgcataac ggttccctgg cgaaaaagca
    15601 tgggcgcgat tatacccaaa cagatgtgcc atttgctttt ttctgcgcca cggaaatcaa
    15661 taacctgaag atatgtgcga cgagcttttc ataaatctgt cataaatctg acgcataatg
    15721 acgtcgcatt aatgatcgca acctatttat tacaacaggg caaatcatgg cgagacgtat
    15781 tctggtcgta gaagatgaag ctccaattcg cgaaatggtc tgcttcgtgc tcgaacaaaa
    15841 tggctttcag ccggtcgaag cggaagatta tgacagtgct gtgaatcaac tgaatgaacc
    15901 ctggccggat ttaattctcc ttgactggat gttacctggc ggctccggta tccagttcat
    15961 caaacacctc aagcgcgagt cgatgacccg ggatattcca gtggtgatgt tgaccgccag
    16021 aggggaagaa gaagatcgcg tgcgcggcct tgaaaccggc gcggatgact atatcaccaa
    16081 gccgttttcg ccgaaggagc tggtggcgcg aatcaaagcg gtaatgcgcc gtatttcgcc
    16141 aatggcggtg gaagaggtga tcgagatgca ggggctaagc ctcgacccga catctcaccg
    16201 ggtgatggcg ggcgaagagc cactggagat ggggccgaca gaatttaaac tgctgcactt
    16261 ttttatgacg catcctgagc gcgtgtacag ccgcgagcag ctgttaaacc acgtctgggg
    16321 aactaacgtg tatgtggaag accgcacggt cgatgtccac attcgtcgcc tgcgtaaagc
    16381 actggagccc ggcgggcatg accgcatggt gcagaccgtg cgcggtacag gatatcgttt
    16441 ttcaacccgc ttttaacgcc ttgctcatcg gacgcagagc agggcttatg atttcttaac
    16501 tggagtatct tacgtgctgg aacggctgtc gtggaaaagg ctggtgctgg agctgctact
    16561 ttgctgcctc ccggctttca tcctgggtgc attttttggt tacctgccct ggtttttgct
    16621 ggcatcggta acaggactgc ttatctggca tttctggaat ttattgcgcc tttcatggtg
    16681 gctgtgggtg gatcgcagta tgaccccgcc accggggcgt ggtagctggg aaccgctact
    16741 atacggctta caccagatgc agctgcgaaa taaaaaacgc cgccgcgaac tgggcaatct
    16801 gattaaacgc tttcgtagtg gcgcggagtc gctgcccgac gcagtagtgc tgaccacgga
    16861 agagggcggt attttctggt gtaacggtct ggcgcaacag attcttggtt tgcgctggcc
    16921 ggaagataac gggcagaaca tccttaacct gctgcgttac ccggagttta cgcaatatct
    16981 gaaaacgcgt gatttttctc gcccgcttaa tctggtgctc aacaccgggc ggcatctgga
    17041 aattcgcgtt atgccttata cccacaaaca gttgctgatg gtggcgcgtg atgtcacgca
    17101 aatgcatcaa ctggaagggg cgcggcgtaa cttttttgcc aacgtgagcc atgagttacg
    17161 tacgccattg accgtgttac agggttacct ggagatgatg gatgagcagc cgctggaagg
    17221 cgcggtacgc gaaaaagcgt tgcacaccat gcgcgagcag acccagcgga tggaagggtt
    17281 ggtgaagcaa ttgctgacgc tgtcgaaaat agaagccgca ccgacgcatt tgctcaatga
    17341 aaaggttgat gtgccgatga tgctgcgcgt cgttgagcgc gaggctcaga ctctgagtca
    17401 gaaaaaacag acatttacct ttgagataga taacggcctc aaggtgtcag gcaacgaaga
    17461 tcagctacgc agtgcgattt cgaacctggt ctataacgcc gtgaatcata cgccggaagg
    17521 cacgcatatc accgtacgct ggcagcgagt gccgcacggt gccgaattta gtgttgaaga
    17581 taacggaccg ggcattgcac cggagcatat tccgcgcctg accgagcgtt tttatcgcgt
    17641 tgataaagcg cgttcccggc aaaccggtgg tagcggatta gggttagcga tcgtgaaaca
    17701 tgcggtgaat catcacgaaa gtcgcctgaa tattgagagt acagtaggaa aagttacacg
    17761 tttcagtttt gttatcccgg aacgtttaat tgccaaaaac agcgattaat tcgcctttgt
    17821 catcttttat tgccataagc cagccgatgc tggcttattt tctttgcagt cgaaatacgg
    17881 gcgttagatt ttacaacggt tggtgatttt ttgttcgcat gattagccat gtctttttca
    17941 ttgaaatagt gttttatact ggttggtgat ttcttatcgc tatatacctc tggtttttag
    18001 atcccttctt gctttaaaac gttataagcg tttaaattgc gcttcaggtg ctgtcatact
    18061 gactgcatta acgcggtaaa tcgaaaaact attcttcgcc gcgcctggtt gggagtattt
    18121 cccgctaaaa ttgtttaaat ataccgctgt atcatcccca gggattggca caaaaattta
    18181 acgttacaac accacatcca caggcagtat gatttatgac ccatcaatta agatcgcgcg
    18241 atatcatcgc tctgggcttt atgacatttg cgttgttcgt cggcgcaggt aacattattt
    18301 tccctccaat ggtcggcttg caggcaggcg aacacgtctg gactgcggca ttcggcttcc
    18361 tcattactgc cgttggcctg ccggtattaa cggtggtggc gctggcaaaa gttggcggcg
    18421 gtgttgacag cctcagcacg ccaatcggta aagttgctgg cgtactgctg gcaacggtgt
    18481 gttacctggc ggtggggccg ctgttcgcta cgccgcgtac agctaccgtt tcttttgaag
    18541 tggggattgc gccgctgacg ggtgattccg cactgccgct gtttatttac agcctggtct
    18601 atttcgctat cgttattctg gtttcgctct atccgggcaa gctgctggat accgtgggca
    18661 acttccttgc gccgctgaaa attatcgcgc tggtcatcct gtctgttgcc gcaattatct
    18721 ggccggcggg ttctatcagc acggctactg aggcttatca aaacgctgcg ttttctaacg
    18781 gcttcgtcaa cggctatctg accatggata cgctgggcgc aatggtgttt ggtatcgtta
    18841 ttgttaacgc ggcgcgttct cgtggcgtta ccgaagcgcg tctgctgacc cgttataccg
    18901 tctgggctgg cctgatggcg ggtgttggtc tgactctgct gtacctggcg ctgttccgtc
    18961 tgggttcaga cagcgcgtcg ctggtcgatc agtctgcaaa cggtgcggcg atcctgcatg
    19021 cttacgttca gcataccttt ggcggcggcg gtagcttcct gctggcggcg ttaatcttca
    19081 tcgcctgcct ggtcacggcg gttggcctga cctgtgcttg tgcagaattc ttcgcccagt
    19141 acgtaccgct ctcttatcgt acgctggtgt ttatcctcgg cggcttctcg atggtggtgt
    19201 ctaacctcgg cttgagtcag ctgattcaga tctctgtacc ggtgctgacc gccatttatc
    19261 cgccgtgtat cgcactggtt gtattaagtt ttacacgctc atggtggcat aattcgtccc
    19321 gcgtgattgc tccgccgatg tttatcagcc tgctttttgg tattctcgac gggatcaaag
    19381 catctgcatt cagcgatatc ttaccgtcct gggcgcagcg tttaccgctg gccgaacaag
    19441 gtctggcgtg gttaatgcca acagtggtga tggtggttct ggccattatc tgggatcgcg
    19501 cggcaggtcg tcaggtgacc tccagcgctc actaaatcac tgaacatttg ttttaaccac
    19561 ggggctgcga tgccccgtgg ttttttattg tgttgatggg ttaggaattg atggaaagta
    19621 agaacaagct aaagcgtggg ctaagtaccc gccacatacg ctttatggca ctgggttcag
    19681 caattggcac cgggttgttt tacggttcgg cagacgccat caaaatggcc ggtccgagcg
    19741 tgttgttggc ctatattatc ggtggtgtcg cggcgtatat cattatgcgc gcgctggggg
    19801 aaatgtcggt acataacccg gccgccagct ctttctcgcg ttatgcgcag gaaaacctcg
    19861 gcccgctggc tggttacatt accggctgga cctactgctt tgaaatcctt attgtcgcca
    19921 tcgccgatgt gaccgctttt ggtatctata tgggtgtctg gttcccgacg gtgccgcact
    19981 ggatttgggt actgagcgtg gtgctgatca tttgcgccgt aaacctgatg agcgtgaagg
    20041 tattcggtga gctggagttc tggttctcgt tctttaaagt cgccaccatc atcatcatga
    20101 ttgtcgccgg tttcggcatc atcatctggg ggattggcaa cggcgggcaa ccgaccggta
    20161 ttcataacct gtggagcaac ggcggcttct tcagtaacgg ctggcttggg atggtgatgt
    20221 cgttgcaaat ggtgatgttt gcttacggtg ggatcgaaat tatcgggatt accgccggtg
    20281 aagcgaaaga tcctgagaaa tcgattccgc gtgcgattaa ctccgtgccg atgcgtattc
    20341 tggtgttcta cgtcggtacg ctgttcgtca ttatgtctat ctacccgtgg aatcaggttg
    20401 gcactgccgg tagcccgttc gtactgacgt tccagcatat gggcattacc tttgccgcca
    20461 gcattcttaa ctttgttgtg ctgaccgctt cgctgtcggc aattaacagt gacgtatttg
    20521 gcgtaggccg tatgctccac ggtatggcag agcagggcag cgcaccaaaa attttcagca
    20581 aaacctcgcg tcgcggtatt ccgtgggtta cggtgctggt gatgactacc gcgctgctgt
    20641 ttgctgtgta tctgaactac atcatgccgg aaaacgtctt cctggtgatc gcttcgctgg
    20701 caaccttcgc cacggtgtgg gtgtggatta tgatcctgct gtcgcaaatc gccttccgtc
    20761 gccgtttacc gccagaagaa gttaagtcgc tgaaatttaa agtaccgggt ggggtagcaa
    20821 ccaccatcgg cggtttgatt ttcctgctct ttattatcgg gttgattggt tatcacccgg
    20881 atactcgtat ctcgctgtat gtcggtttcg cgtggattgt tgtgctgttg attggctgga
    20941 tgtttaagcg ccgccacgat cgtcagctgg ctgaaaacca ctaatccttg cgttctgtaa
    21001 gccggttcag gaggaaatcc tgatccggct attccgaaag ttatccgccc ccgtcctcct
    21061 cccccaaata tccttcagat gatgagtgat cctgcattag gctatggcaa ggtgatcaga
    21121 ttttcatcac aggggaatta tgatgttaaa tgcatggcac ctgccggtgc ccccatttgt
    21181 taaacaaagc aaagatcaac tgctcattac actgtggctg acgggcgaag acccaccgca
    21241 gcgcattatg ctgcgtacag aacacgacaa cgaagaaacg tcagtaccga tgcataagca
    21301 gcgcagtcag ccgcagcctg gtgttaccgc atggcgggcg gcgatcgatc tctccggcgg
    21361 actacctcgg cggcgttata gtttcaaatt actgtggcac gatcgccagc gttggtttac
    21421 accgcagggc ttcagccgaa tgccgccggc acgactggag cagtttgccg tcgatgtacc
    21481 ggatatcggc ccacaatggg ctgcggatca gattttttat cagatcttcc ctgatcgttt
    21541 cgcccgtagt cttcctcgtg aagctgaaca ggatcatgtc tattaccatc acgcagccgg
    21601 acaagagatc atcttgcgtg actgggatga accggtcacg gcgcaggcgg gcggatcaac
    21661 gttctatggc ggcgatctag acgggataag cgaaaaactg ccgtatctga aaaaacttgg
    21721 cgtgacggcg ctgtatctca atccggtgtt taaagctccc agcgtacata aatacgatac
    21781 cgaggattat cgccatgtcg atccgcagtt tggcggtgat ggggcgttgt tgcgtttgcg
    21841 acacaatacg cagcagctgg gaatgcggct ggtgctggac ggcgtgttta accacagtgg
    21901 cgattcccat gcctggtttg acagacacaa tcgcgggacg ggtggtgctt gtcacaaccc
    21961 cgaatcgccc tggcgcgact ggtactcgtt tagtgacgat ggcacggcgc tcgactggct
    22021 gggctatgcc agcttgccga aactggatta tcagtcggaa agtctggtga atgaaattta
    22081 tcgtggggaa gacagtatcg tccgccactg gctgaaagcg ccgtggaata tggacggctg
    22141 gcggctggat gtggtgcata tgctggggga agcgggtggg gcgcgcaata atttgcagca
    22201 cgtagccggg atcaccgaag cggcgaaaga aacccagccg gaagcgtata tcgttggaga
    22261 gcattttggc gatgcacggc aatggttaca ggccgatgtg gaagatgccg ccatgaacta
    22321 tcgcggcttc acattcccgt tgtggggatt tcttgccaat accgacatct cttacgatcc
    22381 gcagcaaatt gacgcccaaa cctgtatggc ctggatggat aattaccgcg ccggactttc
    22441 tcatcaacag caactgcgta tgtttaatca gctcgatagc cacgatactg cgcgatttaa
    22501 aacgctgctc ggtcgggata ttgcgcgcct gccgctggcg gtggtctggt tgttcacctg
    22561 gcctggtgta ccgtgcattt attacggtga tgaagtggga ctggatggta aaaacgatcc
    22621 gttttgccgt aaaccgttcc cctggcaggt ggaaaagcag gatacggcgt tattcgcgct
    22681 gtaccagcga atgattgcgc tgcgtaagaa aagtcaggcg ctgcgtcgtg gcggctgtca
    22741 ggtactgtat gcggaagata acgtggtggt atttgtccgc gtgctgtatc agcagcgtgt
    22801 actggtggca atcaaccgtg gcgaagcctg tgaagtggtg ctacccgcgt cgccgttgct
    22861 taatgtcgcg caatggcaac gcaaagaagg tcacgggcaa ctgacagacg ggattctggc
    22921 tttgcctgcc atttcggcta cggtgtggat gaattaacgt cttataacgc cttgcgtgac
    22981 gcctgctcca tcatccgcgg ataaaactgc cagaagcagg tttcgagagc atcgtaatgg
    23041 gcgtctaaat cgtaccagga gttacgcagg gcatccagac gtgggcgacg gcttgccatg
    23101 ccgtttaaca cgctctggat gaaatccata tcgcgatagc gcaccaacca ctgctctgac
    23161 cataagtagt tgttcagatt gataaagcgt ggcggtgagt ctggaagaat agtcatcact
    23221 tgctcccggg cataacaggt aaattcctgt agcggaaaat cgggcgacag ctgcgaccag
    23281 tggcgggaca gaaagtgatc ccacatcaca tccagcgtaa taggcgcgac acggcgcgtt
    23341 tcattacgaa accactcccg cgcttcgcgg acttctggca gattgtcggt taatacgtcg
    23401 attcgccgat gcatatgaat gccagccacg acgtcgggcg gaaaactttc ttcaggattt
    23461 ccgcgtacga aatcagccag taaattgccg gaaagcgagc tttccgcgag atgggctaaa
    23521 tgcaggtgag ctaaaaaatt catcgattat attctatcca aaggggggta aaggttgcag
    23581 ggagagcgcc ccggcactag actacccgcc tcttatttta gtctgagtca gtgtcatgcg
    23641 cgttaccgat ttctcttttg aattgcccga atccctgatt gcccactatc ccatgcctga
    23701 acgcagtagc tgtcgtttac tgtcgctgga cgggccgacg ggcgcgctga cgcacggtac
    23761 tttcaccgat ttactcgata agctcaaccc cggcgatctt ctggttttta ataatacccg
    23821 cgtgatcccg gcgcgtctgt ttgggcgtaa agccagcggc ggcaagattg aagtgctggt
    23881 tgagcggatg ctcgacgaca aacgcattct tgcgcatatt cgcgcctcga aagcgccaaa
    23941 acctggcgca gaactgctgc tgggcgatga cgaacgtatt aacgcaacaa tgaccgcgcg
    24001 ccacggcgca ctgtttgaag tcgaatttaa tgatcaacgt tcggtgctgg atattctcaa
    24061 cagcatcggc catatgccgc tgccgccgta catcgaccgt ccggacgaag atgcagaccg
    24121 cgaactttat caaacggttt atagcgaaaa acccggcgcg gtagcagcac cgaccgccgg
    24181 actgcacttt gacgaacctt tgctggaaaa attacgcgcc aaaggcgtgg agatggcgtt
    24241 tgtgacgttg cacgttggtg cgggcacctt ccagccggtg cgcgtcgaca ccattgaaga
    24301 tcacatcatg cactcggaat acgctgaagt gccgcaggat gtggtagacg cggtactggc
    24361 ggcgaaagcg cgcggtaacc gggtgattgc ggttggtacc acctcagtac gttcgctgga
    24421 aagtgcggct caggcagcga aaaacgatct cattgaaccg ttctttgacg atacccaaat
    24481 ctttatctat ccgggtttcc agtacaaagt ggtcgatgcg ctggtgacga acttccacct
    24541 tccggaatcg acgctgatta tgctggtttc ggcatttgct ggttatcaac acaccatgaa
    24601 tgcctataaa gcggcggtag aagagaaata tcgctttttt agttacggtg atgcgatgtt
    24661 tatcacgtac aatccgcagg caattaatga gcgcgttggg gagtaattcc gcggcgctgg
    24721 tttaaacgtt ggactgtttt tctgacgtag tggagaaaaa atgaaatttg aactggacac
    24781 caccgacggt cgcgcacgcc gtggccgcct ggtctttgat cgtggcgtag tggaaacgcc
    24841 ttgttttatg cctgttggca cttacggcac cgtaaaaggg atgacgccgg aagaagttga
    24901 agccactggc gcacaaatta ttctcggtaa caccttccac ctgtggctgc gcccgggcca
    24961 ggaaatcatg aaactgcatg gcgatttaca cgattttatg cagtggaaag ggccgattct
    25021 taccgactcc ggcggcttcc aggtcttcag ccttggtgat attcgtaaaa tcaccgaaca
    25081 gggcgtgcac ttccgtaacc cgatcaacgg cgacccgatt ttcctcgacc cggaaaagtc
    25141 gatggagatt cagtacgatc ttggttcgga tatcgtcatg atctttgatg agtgtacgcc
    25201 gtatcctgct gactgggatt acgcaaaacg ctccatggag atgtctctgc gttgggcgaa
    25261 gcgtagccgt gagcgttttg acagtctcgg aaacaaaaat gcactgtttg gtatcattca
    25321 gggcagcgtt tacgaagatt tacgtgatat ttctgttaaa ggtctggtag atatcggttt
    25381 tgatggctac gctgtcggcg gtctggctgt gggtgagccg aaagcagata tgcaccgcat
    25441 tctggagcat gtatgcccgc aaattccggc agacaaaccg cgttacctga tgggcgttgg
    25501 taaaccagaa gacctggttg aaggcgtgcg tcgcggtatc gatatgtttg actgcgtaat
    25561 gccaacccgc aacgcccgaa atggtcattt gttcgtgacc gatggcgtgg tgaaaatccg
    25621 caatgcgaag tataagagcg atactggccc actcgatcct gagtgtgatt gctacacctg
    25681 tcgcaattat tcacgcgctt acttgcatca tctcgaccgt tgcaacgaaa tattaggcgc
    25741 gcgactcaat accattcata accttcgtta ctaccagcgt ttgatggcgg gtttacgcaa
    25801 ggctattgaa gagggtaaat tagagagctt cgtaactgat ttttaccagc gtcaggggcg
    25861 agaagtacca cctttgaacg ttgattaata ttaataatga gggaaattta atgagctttt
    25921 ttatttctga tgcggtagcg gcaacgggtg caccggcgca aggtagcccg atgtctttga
    25981 ttttgatgct ggtggtattc ggtctgattt tctatttcat gatcctgcgt ccacagcaga
    26041 agcgcaccaa agaacacaaa aagctgatgg actccatcgc taaaggtgat gaagtgctga
    26101 cgaacggtgg cctggtcggt cgcgtaacca aagtagcgga aaacggctac attgctatcg
    26161 cgctgaatga caccactgaa gtagttatta aacgtgactt cgtagctgcc gttctgccga
    26221 aaggcaccat gaaggcgctg taattaaaat ttttccctaa gggaattgcc gtgttaaacc
    26281 gttatccttt gtggaagtac gtcatgctga tcgtggtgat tgtcatcggt ctgctgtatg
    26341 cgcttcccaa cctgtttggt gaggatccgg ctgttcagat cactggtgcg cgcggagtcg
    26401 ccgccagtga gcaaacgctg atccaggtcc agaaaacgtt acaagaagaa aaaataactg
    26461 ctaagtctgt ggcactggaa gagggcgcta ttcttgcgcg cttcgactcc actgacaccc
    26521 agctgcgcgc tcgtgaagca ttaatgggcg ttatgggtga caaatacgtc gtggcgctta
    26581 accttgcccc ggcaacgccg cgctggctgg cagctattca cgctgagccg atgaagctcg
    26641 gccttgacct gcgtggcggc gttcacttcc tgatggaagt ggatatggac accgcgcttg
    26701 gcaaactcca ggaacaaaac atcgatagcc tgcgcagcga cctgcgcgaa aagggcatcc
    26761 cgtataccac cgttcgtaaa gaaaacaact acggcctgag catcactttc cgcgatgcta
    26821 aagctcgtga tgaagccatt gcgtatctga gcaagcgcca tccggacctg gtgattagca
    26881 gccagggcag caaccagctg cgtgcggtaa tgagcgatgc tcgcctgagt gaagcgcgtg
    26941 aatatgcggt gcagcagaac attaatatcc tgcgtaaccg tgtaaaccaa cttggcgtgg
    27001 cggagccggt ggttcagcgt cagggtgctg accgtatcgt tgttgaactg ccaggtattc
    27061 aggacacagc gcgtgcgaaa gagattctgg gtgcgacggc aacgctggaa ttccgtctgg
    27121 taaacaccaa cgttgatcag gccgctgcgg catccggtcg cgtaccgggc gactctgaag
    27181 tgaaacagac ccgcgaaggt cagccggttg tgctgtacaa acgcgtaatt ctgaccggtg
    27241 accatatcac cgactccact tccagccagg acgaatacaa ccagccgcag gttaacatct
    27301 cgctcgatag cgcgggtggt aacatcatgt ctaacttcac caaggacaac atcggcaaac
    27361 cgatggcaac cctgtttgtg gagtacaaag acagcggtaa gaaagatgca aacggtcgtg
    27421 cggttctggt gaaacaggaa gaggtgatta atatcgccaa catccagtct cgtctgggta
    27481 acagcttccg tattactggc atcaataacc caagcgaagc gcgtcagctt tcactgctgc
    27541 tgcgtgccgg tgcgttgatc gcgccgattc agattgttga agaacgtacc attggcccaa
    27601 ccctgggtat gcagaacatt gagcaggggc tggaggcttg ccttgccggt ctgctggttt
    27661 ctattctgtt catgatcatc ttctataaga agtttggtct gattgcgacc agtgctctga
    27721 ttgccaactt gatcttgatt gtcggtatta tgtcgctgtt gccaggcgca acgctgagta
    27781 tgccaggtat cgcgggtatc gtcttaaccc ttgcggtagc ggtcgatgcg aacgtactga
    27841 tcaacgaacg tattaaagaa gagttgagca acggacgtac tgttcaacag gcaattgatg
    27901 aaggttatcg tggcgcattc agttctatct tcgatgcgaa catcaccacg ctgattaaag
    27961 tcatcatcct gtacgcagtg ggtaccgggg caattaaagg gttcgcgatt actaccggta
    28021 tcggtgtggc gacgtcgatg tttaccgcga ttgtcggtac gcgtgccatc gtaaacctgc
    28081 tatatggcgg caagcgcgtc aagaagctgt caatctgagg agtgcgatgt ggcacaggaa
    28141 tatactgttg aacaactaaa ccacggccgt aaagtctatg actttatgcg ctgggactac
    28201 tgggcttttg gcatctctgg tctgctgtta atcgccgcta tcgttattat gggcgtgcgc
    28261 ggctttaact gggggctgga tttcaccggt ggtacggtta ttgaaattac gctcgaaaaa
    28321 ccggctgaaa ttgacgtaat gcgtgatgca ttgcaaaaag ccggttttga agagccgatg
    28381 ctgcaaaact ttggtagcag ccacgacatc atggtccgta tgccgcctgc tgaaggcgaa
    28441 accggcggtc aggtgctggg cagccaggtc ctgaaggtga ttaacgaatc caccaatcag
    28501 aatgcggcag tgaagcgtat tgagttcgtc ggtccgagcg tgggggcaga ccttgcgcaa
    28561 accggtgcga tggcgttgat ggcggcgctg ctgtctatcc tcgtgtacgt aggtttccgc
    28621 tttgagtggc gactggcggc aggggttgtt attgcgctgg cacacgacgt tatcattacg
    28681 ctgggtattt tgtcgttatt ccatatcgag attgacctga ccattgtggc atcgttgatg
    28741 tcagttatcg gttactcgct taacgacagt atcgtggtat cggaccgtat tcgtgaaaac
    28801 ttccgcaaga tccgtcgcgg tacgccttac gaaatcttta acgtgtcctt gacccagacg
    28861 ctgcaccgta ccttgatcac atccggtact accttgatgg ttatcctgat gctgtacctc
    28921 ttcggtggtc cggtactgga aggcttctcg ctgaccatgc ttatcggtgt ttccatcggt
    28981 actgcatctt ccatctatgt ggcatcggcg ctggctctga aactgggtat gaagcgcgaa
    29041 cacatgttgc agcagaaagt ggaaaaagaa ggggcggatc agccgtcaat tctgccttaa
    29101 tcaagtttca gctgatgttg aaaatcccgg tcagaagatc gggatttttt ttgatgtaag
    29161 gtgattgcga aaatccccgc atcttgggaa actgcgcgta accctacatt tcatccaggt
    29221 aactttttca tggctatcat cccaaaaaac tatgcgcggt tagaaagcgg ctatcgcgaa
    29281 aaagcattaa aaatctatcc gtgggtctgc ggtcgctgtt cccgcgagtt tgtttattcc
    29341 aacctgcgtg aacttaccgt tcaccacatt gatcacgacc atactaataa cccggaagat
    29401 ggcagtaact gggaattact ttgtctctat tgccacgatc atgagcattc gaaatacacc
    29461 gaagcggatc agtatggtac gaccgttatc gcaggggaag atgcgcagaa agatgtcggt
    29521 gaagcgaagt acaacccatt cgctgatttg aaagcgatga tgaacaagaa gaagtgataa
    29581 aaaatgccgg atatgaaatc cggcattctc acaattagaa gttgtaacct actaccagat
    29641 aaccacccca gccggtagag cgaacgttga agttgccgtt gccgaagttc agttctgcat
    29701 cgtcattcca ctgaccaccg ttatgccagt aacgggcaac gacagagtag tgccagtgat
    29761 cgtaattcag cgccagaata tggctggacg caatagagtt attggtacgt gttttaatac
    29821 cgttgttcgc ataaccgctg tcatcgccca ggtcggaacc ccagtcgaag ttggtgaagc
    29881 cgatgtagct cagctggccg ccccacagat cggtaatcgg cacaaagtac ttcactttga
    29941 aacggtaacc gtcccactcg ttttcgttcg ctgcgccgta gttctgccac tggtatttcg
    30001 catagacgtt catggacagg ctcatcggca gaccagtatc gatgtcggta cccagaccca
    30061 tgtaccaggt gctctggcgg ccatctttat tacgacccat gtcgtaaatg tagttgttcg
    30121 cgaagtacca ctctttgaac ggaccaaagc taaggtcagt attggtcagc ttgtcgatgg
    30181 agaaacgcgg ttcgatttcc ataaacagcg gagaaccgtg gttccagata ccttttgcat
    30241 cggagttacc gccgaagaat accggcgcat ccgcataacc atagaagtcg aaccagtctt
    30301 ttttagcgaa tgcttcgtac tcaaggtagg tatcgttgcg gagctgcggt ccgaaacggg
    30361 tgtgatagct tccgacaacg ttaacgctct ggtgccacca gtcggaaaga tactgcggtt
    30421 tgtcgttttc agctgcgttg acagtaaaag acgaagagag cgccagtacc gcaccggctg
    30481 ccagtaatgt ttttttcata tgtatgccac tgtttgaaaa tcccttgcgg gagtgaaaaa
    30541 ggcgcaaatt gcgtttctaa atatttcgta tacagaggag cctattataa aaatcattgc
    30601 tcacaaaaat atgtttcgtt tcacagttct atcatttacg taatcgattg cgttcacgtt
    30661 tacacacatt cgggcgggga ttgtactgac tttcaccctt gttgcaaact tttactatca
    30721 atccaggtga tgtaagcgga gtaacaaaat gacggagaaa gatgttgcgg gacgcacaat
    30781 atcgccccgc aatacgttta ctgcataaca ggttgaatgt cgtggatacg tacaaagcct
    30841 aactgatcca cagacatacc ctttaactgt aacggaatat caacatcgct gggggcgagg
    30901 acgctggcag gtgcgttaat caattgattc tgcacattga tttcctgaaa gttgtctatt
    30961 gttccctgaa tttgcccata ttcaacggtt ccgctgaatg cgggtaaagg gtcattggat
    31021 tcgccctgaa tacgtagcgt cagggtagtg ccatctgtgt caggcgtaat attcaccagc
    31081 gacatacgta aagtaccgat ctggctttcc agtcttgctg gtgtttttgc tccaggcaga
    31141 aggtaaaccc cactgctgga ttttgcattc agcttgtttt gctgagtgat tttgaccgtt
    31201 tctttattga gttgcgtcat ctcctggttc agcgtgctga cgctgtgttt catctgtcgc
    31261 acttcacttt gctgcacaca ggcgctaagg ctgaacaggc ttcccagcag gagcagtcga
    31321 aaaacgttag tattcatagg tcgtcccttt ctcgtcgcaa ttagcctaat ggtagagggt
    31381 aaaagcagtg aaagcatcga tcctttatct caaaagcgtt gcgcctttgt tgtatcgtca
    31441 gttcagggta aaatagattt ctgttaacca cctggtcagg acgccgtatg cattgcccat
    31501 tctgtttcgc cgtggacact aaggtaattg actctcgtct cgtgggcgag ggttcatccg
    31561 tacgccgccg tcggcagtgt ctggtgtgta atgaacgttt caccaccttt gaagtggcgg
    31621 agctggttat gccgcgtgtt gtaaaaagca acgacgtgcg tgaaccgttt aatgaagaga
    31681 aattacgtag cggaatgctg cgggcgctgg aaaaacgtcc ggtgagttcc gatgacgtcg
    31741 aaatggcaat caatcatata aaatcgcagc tgcgcgccac cggtgagcgc gaagtgccga
    31801 gcaagatgat tggcaatctg gtgatggagc aattgaaaaa gctcgataaa gtcgcctata
    31861 tccgttttgc ctctgtctac cgcagtttcg aagatatcaa agaatttggc gaagagatcg
    31921 cgcgcctgga ggactaagcc gtgcaggacg agtattacat ggcgcgggcg ctaaagctgg
    31981 cgcaacgagg acgttttacc acgcatccca acccgaatgt cgggtgcgtc attgtcaaag
    32041 atggcgaaat tgtcggtgaa ggttaccatc aacgcgcggg tgaaccgcat gccgaggtac
    32101 acgcgttgcg catggccggc gaaaaagcca aaggcgcaac ggcgtatgtc acactcgaac
    32161 cctgtagcca tcatggtcgt acgccaccgt gttgtgatgc attaattgct gcgggcgtgg
    32221 cgcgcgtggt tgctgcaatg caagacccca atccgcaggt cgctggacgt ggactttacc
    32281 gtctgcaaca ggctggcatt gacgtcagcc acgggctgat gatgagtgaa gctgagcaat
    32341 tgaataaagg ctttctcaaa cggatgcgca ccggctttcc ttatattcag ttaaaacttg
    32401 gcgcatcgct tgatggtcgc acggcgatgg cgagtggcga aagccagtgg atcacttcac
    32461 ctcaggcgag gcgcgacgta caacgactgc gcgcgcaaag tcatgccatt ttaaccagca
    32521 gcgccacggt gctggcggat gatccagcct taacagtgcg ttggtctgaa ctggatgaac
    32581 aaactcaggg actctacccg caacaaaatc tccgccagcc ggtacgtatt gtgattgata
    32641 gccaaaatcg cgtgacgccg gaacatcgca ttgtgcagca gcccggcgaa acctggttcg
    32701 cgcgtacgca ggaagattct cgtgagtggc cggaaacggt gcgtaccttg ctgattccag
    32761 agcataaagg tcacctggat ctggttgtac taatgatgca actgggtaaa cagcagatta
    32821 acagcatctg ggtggaagcg gggccaacgc tcgctggcgc attgctacag gcgggtttag
    32881 tcgatgagct gattgtctat atcgcaccta aactattagg caacgacgcc cgtggattat
    32941 gcacgctgcc agggcttgag aaattagccg acgctcccca atttaaattc aaagagatac
    33001 gtcatgtagg cccggatgtt tgcctgcatt tagtgggtgc atgatctccc ggctcgaaag
    33061 ggaagcagcg cacgaaatat tatgctaaaa tccgcccccc tgcggggcca tactcgaacc
    33121 cgaaggaaga aaatgaacat tattgaagct aacgttgcta ccccggacgc tcgcgtcgcc
    33181 atcaccatcg cgcgtttcaa caactttatc aatgacagcc tgctggaagg tgcaattgac
    33241 gcactgaaac gtatcggtca ggtaaaagat gaaaacatta ccgttgtttg ggtgccaggt
    33301 gcctatgagc tgccgctggc ggcgggtgca ctggctaaaa ccggtaaata cgacgcggtg
    33361 attgcgctgg gtacggttat tcgtggtggc actgcccact ttgaatatgt cgctggtggt
    33421 gcaagcaacg gcctggcgca tgttgcccag gacagcgaaa ttccggttgc ttttggggtt
    33481 ctgaccactg aaagcattga acaagcgatc gaacgtgctg gcaccaaagc tggcaacaaa
    33541 ggtgcagaag ctgcactgac cgcgcttgaa atgattaatg tattgaaagc catcaaggcc
    33601 tgaaattagt aaggggaaat ccgtgaaacc tgctgctcgt cgccgcgctc gtgagtgtgc
    33661 cgtccaggcg ctctactcct ggcagttgtc ccagaacgac atcgctgatg ttgaatacca
    33721 gttcctggct gaacaggatg taaaagacgt tgacgtcctg tacttccgtg agctgctggc
    33781 cggggtggcg actaacaccg catacctcga cggactgatg aagccgtacc tgtcccgtct
    33841 gctggaagaa ctgggccagg tagaaaaagc agtactgcgc attgcgctgt acgagctgtc
    33901 taaacgtagc gatgtgccat acaaagtggc cattaacgaa gcgatcgaac tggcgaaatc
    33961 gttcggcgca gaagacagcc ataagttcgt caacggcgta ctcgataaag cagcacctgt
    34021 gattcgccct aacaaaaagt aatatccagg ccggtagatt cacggaagac cgttccatga
    34081 tcgccggcct tttctttttt acctgctgag gcataacgta tggcatgtgg cgagttctcc
    34141 ctgattgccc gttattttga ccgtgtaaga agttctcgtc ttgatgtcga actgggcatc
    34201 ggcgacgatt gcgctcttct caatatcccc gagaaacaga ccctggcgat cagcactgat
    34261 acgctggtgg cgggcaacca cttcctccct gatatcgatc ctgctgatct ggcttataaa
    34321 gcactggcgg tgaacctaag cgatctggca gcgatggggg ccgatccggc ctggctgacg
    34381 ctggcattaa ccttaccgga cgtagacgaa gcgtggcttg agtccttcag cgacagcttg
    34441 tttgatcttc tcaattatta cgatatgcaa ctcattggcg gcgataccac gcgtgggcca
    34501 ttatcaatga cgttgggtat ccacggcttt gttccgatgg gacgagcctt aacgcgctct
    34561 ggggcgaaac cgggtgactg gatctatgtg accggtacac cgggcgatag cgctgccggg
    34621 ctggcgattt tgcaaaaccg tttgcaggtt gccgatgcta aagatgcgga ctacttgatc
    34681 aaacgtcatc tccgtccgtc gccgcgtatt ttacaggggc aggcactgcg cgatctggca
    34741 aattcagcta tcgatctctc tgacggtctg atttccgatc tcgggcatat cgtgaaagcc
    34801 agcgactgcg gcgcacgtat tgacctggca ttgctgccgt tttctgatgc gctttctcgc
    34861 catgttgaac cggaacaggc gctgcgttgg gcgctctctg gtggtgaaga ttacgagttg
    34921 tgtttcacgg tgccggaact gaaccgtggc gcgctggatg tggctctcgg acacctgggc
    34981 gtaccgttta cctgtatcgg gcaaatgacc gccgatatcg aagggctttg ttttattcgt
    35041 gacggcgaac ctgtcacgtt agactggaaa ggatatgacc attttgccac gccataaaga
    35101 tgtcgcgaaa agtcgcctga aaatgagtaa tccgtggcat ctgcttgctg tcggattcgg
    35161 aagtggatta agcccgatcg ttcctggcac gatggggtcg ctggcggcga ttcccttctg
    35221 gtatctgatg acttttttgc cctggcagct ctattcgctg gtggtaatgc tggggatctg
    35281 tatcggcgtc tatctttgtc atcaaacggc gaaagacatg ggcgtgcacg atcacggcag
    35341 tattgtctgg gacgaattta ttggtatgtg gattacgctc atggcgctgc cgaccaatga
    35401 ctggcagtgg gttaccgccg gatttgtgat tttccgtatt ctggatatgt ggaagccgtg
    35461 gccgatccgc tggtttgatc acaatgtaca tggcggcatg gggatcatga ttgacgatat
    35521 tgtcgccggg gtgatctccg ctggcatcct gtattttatc ggtcatcact ggccgctggg
    35581 tattctgtcg tagttgtgca ccgatgcctg atgtgacgct tgtcacgtct catcaggcct
    35641 gcatacctat ttaaatccta cgaccggatg cggtttatac ggcgtttcca gctcggcgat
    35701 ctgttccggc ttcaaggtga tatccaccgc gtttagcagt tcatcaagct gttcctcccg
    35761 agaagttccg ataatcggtg cggcaatgcc cggtttactc aacaaccagg ccagcgcaac
    35821 ttgtgctcgc gttgcaccaa gttcttcact gacgcccgtt aagcgttctg caatttgtgc
    35881 gtcattttca ttgctttctt ggtagagatt tttccccact tcatccgaca ccagccgtgc
    35941 ggtggtttct ccccaaggac gcgtcagacg gccccgcgcc agcggactcc atggaatcac
    36001 cgccacgcct tcctgataac acagcggcag catctcgcgc tcttcttcac gataaatcag
    36061 attgtagtga tcctgcatac tgacaaactg cgcccagccg tgctgttttt gtagttccag
    36121 tgcctgagca aactgcgaag cgtgcatgga tgacgcgccg atataacgtg ctttcccggc
    36181 ttttaccacg tcgttcaggg cttccagcgt ctcttcgatc ggcgtgttgt aatcccagcg
    36241 atgaatttgc aggatatcga cataatccat gccgagacgg agcaggctgt cgtcgataga
    36301 gcgcaaaatt tgtgcacggg ataatccttc cggtaaatca ccaacgcgat ggaacacttt
    36361 ggtcgcaacg accacgtctt cacgacgggc gaaatcccgc agtgcgcgac cgacgatctc
    36421 ttcgctgctg ccatcggaat agctattggc ggtatcaaag aaatttatgc cgccttccag
    36481 tgcgcgttta atgatgggac ggctgctctc ttccggcagt gtccatgcgt gattaccgcg
    36541 atctggctcg ccaaaggtca tacagccgag gcaaagtcgg gaaacacgaa ggtcggtttt
    36601 tcctaagggg ttgtattgca tgctgccact cctgccatga ataatttctt aagcatagca
    36661 ggagtagagt agggattatg ccagccaggc tttgattttg gcttccatac cggtggcatc
    36721 gaggccgagt tcggcgcgca tttcttcctg agttccttgt ggaataaaga agtccggcag
    36781 accaatgttc agcacgggta ctggtttacg atgggccatc agcacttcgt tcacgccgct
    36841 gcctgcacca cccataatgg cgttttcttc tacggtaacc agcgcttcat ggctggcggc
    36901 catttccaga attaacgctt catcaagcgg tttcacgaaa cgcatatcga ccagcgtagc
    36961 gttcagcgat tcagcgactt tcgccgcttc tggcatcagc gtaccaaagt taaggatcgc
    37021 cagtttctca ccacgacgct tcacaatgcc tttgccaatt ggcagttttt ccagtggcgt
    37081 cagttccacg ccaactgcgt tgccgcgcgg gtagcgcacc gcggacgggc catcgttata
    37141 gtgatagccg gtatagagca tctggcgaca ttcgttttca tcgctcgggg tcataatgac
    37201 aatttccggt atgcagcgca ggtaagagag atcaaaagcg ccctggtggg tttgaccgtc
    37261 agcaccaaca atgcccgcac ggtcgatggc gaacaggacc ggaagctttt gaatcgccac
    37321 gtcatgcagc acctgatcat aggcgcgttg caggaaggtg gagtagatcg caacaatggg
    37381 tttgtagcct ccaatcgcca gacccgcagc aaaggtcact gcgtgttgct cggcaattgc
    37441 cacgtcgaag tagcgatccg ggaatttacg tgaaaactcg accatgccgg aaccttcacg
    37501 catcgccgga gtaatcgcca tcagcttgtt atctttcgct gcggtttcgc acaaccagtc
    37561 gccaaagatt tttgaatagc ttggcaaacc gccgctactt ttcggcagac aaccgctgga
    37621 gggatcaaat ttaggcacgg cgtggaaggt gattgggtct ttttctgccg gttcataacc
    37681 acgacctttt ttggtcatga tatgcaggaa ctgcgggcct ttcaggtcgc gcatgttctt
    37741 aagcgtggtg ataagcccca gcacatcgtg accgtccacc gggccgatgt agttaaagcc
    37801 cagctcttca aacaacgtgc caggcactac catgccttta atatgttctt cggtacgttt
    37861 gagcagctct ttaatgggtg gcacgccaga gaaaactttt tttccgcctt cgcgcagtga
    37921 agagtaaagc ttaccggaaa gcagctgcgc cagatggttg ttgagcgcgc caacattttc
    37981 ggaaatcgac atttcattgt cgttgagaac caccagcata tcaggacgga tatcgcccgc
    38041 gtgattcatc gcttcaaacg ccatgccagc agtaatcgcg ccatcgccaa tgacacagac
    38101 ggtgcggcga tttttgcctt ctttctcggc agcaactgcg atcccaattc cggcactgat
    38161 ggaggttgat gaatgcccga cgcttaatac gtcatattcg ctttcgccgc gccacgggaa
    38221 cgggtgcagg ccacctttct gacggatggt gccgattttg tcgcggcgcc cggtcaaaat
    38281 tttatgcgga taagcctgat gccccacatc ccagattaac tggtcaaacg gggtattgta
    38341 gacatagtgc agcgccacgg tcagttcgac cgtgcccagc ccggaggcga agtgcccgct
    38401 ggaacggctc acgctgtcga gtaaatagcg gcgcagttcg tcgcagagtt tcggtaaact
    38461 ctctttcggc aacagacgta actcctgggt ggagtcgacc agtgccaggg tcgggtattt
    38521 ggcaatatca aaactcatca ggggcctatt aatacttatt gtttatttat tacgctggat
    38581 gatgtagtcc gctagcgctt ccagtgccga ggtatcgagt gactgttcag ccagttgttt
    38641 cagcgactga cgggcatcgt cgatcagatc ccgggctttc ttccgggctt gctcaagacc
    38701 cagaagtgca gggtaggtac ttttaccaag ttgctggtcg gcaccctggc gttttcccaa
    38761 cgttgcagta tctcccacca catccaggat gtcatcctga acctggaagg caaggccgat
    38821 gctctctgcg tacttgtcga gtacagacag agcacgacgt cctttatctc cggcgcttaa
    38881 tgcaccaagg cgaacggcgg cgcgaatcaa tgcgccggtt ttatgacgat gaatacgctc
    38941 aagcgcctcc agaggtacgt gtttgccttc cgcgtctaaa tctaatgcct gaccaccgca
    39001 cattccggca ataccgctgg cgctcgccag ttcagaaatc atcgaaattc tgtcgcgatc
    39061 cgacacttcc ggcatatcag catcgcttaa aatcgagaac gccagcgttt gtaaagcatc
    39121 gccagcgaga atcgcgttcg cttcgccaaa cttcacatgg caggtcggca aaccgcgacg
    39181 cagatcgtca tcatccatcg ccggtaaatc atcatgaatt aatgagtaag cgtggataca
    39241 ctcaacggcg gcagcgggtg cgtccagcgt gtttgtgcta acgccgaaca tatgaccggt
    39301 ggcataaacc aggaaaggtc gcaggcgctt accacctaat aatgcgccat actgcatggt
    39361 ttcgaccacg ggagtgttct gaaagggcag tggggcgata aaacggctca gcgcctggtt
    39421 ggcctgctta acgcaggctt cgagttgctg cggaaagtcc attactcatt gtccggtgta
    39481 aaaggggtta gagaggcgtc ttcattgtca gacagcagaa tttgcacgcg ctgttcggct
    39541 tgttgtaatt tggcctgccc ctgacgtgcc agttgcacgc cgcgttcgaa ctcgttcagc
    39601 gcctcttcca gcggcaggtc gccactttcc agacgggtta caatctgttc cagctcgctc
    39661 agcgcctttt caaagctggc gggcgcctca tttttcttcg gcataatgaa tgtctgactc
    39721 tcaatatttt tcgccccgtc atggtaacgg actcagggca aatagcaaat aacgcgcaat
    39781 ggtaaggtga tgtgcgcagc aaagcgatgt tagtggtata cttccgcgcc tggatgcagc
    39841 cgcaggtgtg ggctgctgta tttttcccta tacaagtcgc ttaaggcttg ccaacgaacc
    39901 attgccgcca tgaagtttat cattaaattg ttcccggaaa tcaccatcaa aagccaatct
    39961 gtgcgcttgc gctttataaa aatccttacc gggaacattc gtaacgtttt aaagcactat
    40021 gatgagacgc tcgctgtcgt ccgccactgg gataacatcg aagttcgcgc aaaagatgaa
    40081 aaccagcgtc tggctattcg cgacgccctg acccgtattc cgggcatcca ccatattctc
    40141 gaagtcgaag acgtgccgtt taccgacatg cacgatattt tcgagaaagc gttggttcag
    40201 tatcgcgatc agctggaagg caaaaccttc tgcgtgcgcg tgaagcgccg tggcaaacat
    40261 gattttagct cgattgatgt ggaacgttac gtcggcggcg gtttaaatca gcatattgag
    40321 tccgcgcgcg tgaagctgac caatccggat gtgactgtcc atctggaagt ggaagacgat
    40381 cgtctcctgc tgattaaagg tcgctacgaa ggtattggcg gtttcccgat tggtacccag
    40441 gaagatgtgc tgtcgctcat ttccggtggt tttgactccg gcgtttccag ttatatgttg
    40501 atgcgtcgcg gatgtcgtgt gcattactgc ttctttaacc ttggcggcgc ggcgcatgaa
    40561 attggcgtgc gtcaggtggc gcattatctg tggaaccgct ttggcagctc ccaccgcgtg
    40621 cgttttgtcg ctattaattt cgaacctgtc gtcggggaaa ttctcgagaa aatcgacgac
    40681 ggtcagatgg gtgttatcct caaacgtatg atggtgcgtg ccgcgtctaa agtggctgaa
    40741 cgttacggtg tacaggcgct ggtcactggc gaagcactgg gccaggtgtc cagccagacg
    40801 ctgactaacc tgcgcctgat tgataacgtc tccgacacgc tgatcctgcg tccgctgatt
    40861 tcttacgaca aagagcacat catcaacctg gctcgccaga ttggcactga agactttgcc
    40921 cgcacgatgc ccgaatattg cggtgtgatc tccaaaagcc cgacggtgaa agcggttaaa
    40981 tcgaagattg aagcggaaga agagaagttc gacttcagca ttctcgataa agtggttgag
    41041 gaagcgaata acgttgatat ccgcgaaatc gcccagcaga ccgagcagga agtggtggaa
    41101 gtggaaaccg tcaatggctt cggcccgaac gacgtgatcc tcgatatccg ttctatcgat
    41161 gaacaggaag ataagccgct gaaagtcgaa gggattgatg tggtttctct gccgttctat
    41221 aaactgagca ccaaatttgg cgatctcgac cagaacaaaa cctggctgtt gtggtgtgag
    41281 cgcggggtga tgagccgcct gcaggcgctc tatctgcgcg agcagggctt taacaatgtg
    41341 aaggtatatc gcccgtaatt tgtggttttt acgtcgcatc tggtcagatg cgacgtttgc
    41401 cgcatccgcc actactcgta ataattataa atccctgccg ccatcaccag ttgtgatgcc
    41461 acttcatggg ctttttcacg cccaaccagc aggtcgataa ttttcagacc aaagtcgata
    41521 gctgtacccg gcccctgact ggtcagcaat tttacccgtg catcccagac gacgcgcttg
    41581 tcctgccatt gttcggcggg tattttgtct ttcagcgtcg ggaagccggt catattgcca
    41641 atcgggaaga tatcgtgcgg gaccagcacg gtggctggtg cggcgcaaat agccgcgacg
    41701 atacgcccgg aacggtggaa ctgtttaacg gtttcaacca gcagggtgct atcgcgaaaa
    41761 cactctgcgc ctttaatgcc tccaggcagc acgatcacgt catattcgcc atcagctact
    41821 tcgaccagcg gcgcatccgc cagcagcttc acgccgcgcg agcaggtaat cgccaggttg
    41881 ccatcgctgg cgacgctggc agtggtgact ttgataccgc cgcgaaccag cagatcgata
    41941 gtggtgacgg cttcagtctc ttcactacca ggggcgaggc aaaccagtgc cgatgcgctc
    42001 atattcactc tcctttcttt ttaccatttc aaacaggcgg gtgttttccg gtacggtaat
    42061 cccatgcgcg cgggcgcggc gtaagagaaa accattgata tagtcgattt cggtgtggcg
    42121 cagcgcgcgg atatcctgca acatcgacga gatattttcc gctgtggcat caatcacctg
    42181 catcacgtaa tcacgcaaat cttctgctga agtatgatgt ccttcgcgtt cgatcactgc
    42241 cgcgacttcc tcgcatatct gcataatttc ttgcggatga tgacgtaatt caccgttcgg
    42301 gcaattccag atggcagtca gtggattaat cacacagttg actgccagct tgcgccacag
    42361 ctcggcgcga atattgttat gccaggcaac gtcaggcaac acggtttgca aaatatccgc
    42421 cagataactg taatcgccat cctgttgccg tgccggacca atatgcgtga taccgtttgc
    42481 cacatgaata atgacattgc cgtcgcggcg agcggcatgg gtggtggtgc ccatcagtaa
    42541 tggctgctga atgttttgca actcttcgat ggtgcccatg ccgttgtgaa ttaacagtat
    42601 tggcgtagtt acaggcagtg tggacgcgag gcttttgacg gcatcggaaa cctgccatgc
    42661 tttcagcgtc accaggagca gatcgctggt ggcgagaaaa tcgggatcgt tggcggtcag
    42721 cgattcgtta aatatcgaac catctgtctc aaccagattc acgctacaat aaggttgcgg
    42781 tacgcgcagc cagccctgaa cgtcgtgacc ctgtttacaa agtgctgtaa gccataattg
    42841 ccctaaggca ccgcatccca atacggtaat tttcattgtt cctcctcacc cgcaaccact
    42901 ccgggtgttc aataaggcta tcccttaatt gtgcatgctg ttgcgactat gcacaattaa
    42961 gggatacgtc ctggtgcagg actgttggtt atttaacttt gcaggtatta tgcttcgcat
    43021 caaaaatgaa gggagaggaa aagatgccat ctttcgatat tgtctctgaa gttgatcttc
    43081 aggaagcacg taacgcggtc gataacgcga gccgcgaagt ggagtcccgt tttgacttcc
    43141 gtaatgttga agcctcattt gagctgaacg acgccagcaa aaccatcaaa gtgttgagcg
    43201 agtccgattt ccaggtcaat cagttgctgg atattctgcg tgccaagctg ctgaagcgcg
    43261 gcattgaagg cagttcgctg gatgtaccgg aaaatatcgt tcatagcggt aaaacctggt
    43321 ttgtggaagc gaaactgaaa cagggcattg agagcgcgac tcagaagaaa atcgtcaaaa
    43381 tgatcaaaga cagcaaactg aaagtgcagg cgcaaattca gggcgatgag atccgcgtaa
    43441 cgggtaaatc tcgtgatgat ttgcaggctg tcatggcgat ggtacgtggt ggcgatctcg
    43501 gtcagccgtt ccagttcaaa aacttccgcg attaatcgcg acaacctgcg ctttgttcat
    43561 gtcagatgcg gcatgaacgc ctgatttggc ttacaaaggt ttgtaactca ggaacttagt
    43621 aggcctgata agcgtagcgc agcaggcaat ttcgcctcgc ttttatgcct ggcgaattgc
    43681 ctgttcaacc tcaaagcgat tcgtcacttt gctgtcgatt ttcacatacg ctgaatgttc
    43741 ttcttctgca atcaacactt ctttgacgcc ttcagtttcc agcaaacgta cttctaacgc
    43801 ttcgtttgcg gcagtgtccg ccgggatttc aatgcgcaag ctgctgacat acggcggttc
    43861 tttcatggta ctggcgactg ccagccacac tgcggccagc attgcgccag cgagaaatac
    43921 tccctgaccg tcaaacatgc cgtcaatcca gccgcccagc gaaccgccaa tcgccacgcc
    43981 aagaaactgg ctggtggagt aaacacccat cgccgtacct ttgtaacctg ctggcgattc
    44041 tttactgata agtgaaggca gaagggcttc catcaaatta aacgccacaa agaaaagctg
    44101 cacgccgacc accagttgcc agaactgcgt ttgtgcgttc cacaacacaa tttccgcaac
    44161 cacgatcagc ccgacgcaaa agacaaagac ttgcttcatt ttgcgcttaa cttcagcgta
    44221 gataatgaaa ggcacgaccg agccaaaggc gattaacatc gttgccaggt agaccttcca
    44281 gtgttcagcc gccgggaacc ccgcatcagc catctgtccg ggcagggcaa caaaggtcga
    44341 catcagcaaa atatgcagac acataatgcc aaagttgagt ttcagcagcc gcggttccgc
    44401 cagcacttta ctgaaacttc ctttcaccat tccggactca cgattaagta cgtgagtgct
    44461 actgttgggc acaacccaaa tggtcaacgc aatgccggtt gctgccagaa tagcgatcat
    44521 ccagaacagc gcgtgcagcc caagtttgtg agtgatgatc gggccaagca ccatcgcaat
    44581 ggcaaaggta atgccaaagc tcacgccgat aaacgccatc gctttggtgc ggttttgttc
    44641 gcgcgtgaga tcggaaagca gcgccataac ggcagcggca atcgcaccgg agccttgtag
    44701 cgcccggccc agaataattc cccagatgga gtcagagagc gcagcgataa cgctaccggc
    44761 ggcaaacact gccagcccac cgacaattaa tggtttgcga ccaatgcggt cggaaagcag
    44821 gccaaacgga atctgaaaaa cggcctgagt cagaccataa ataccaatgg caataccgat
    44881 taatgcttcg ctggcacctt gcagagccat gccgtacgtg gtcagaaccg gcagaaccat
    44941 gaacatgccc agcatgcgca acgagaatac ggtccctaaa ccccaggtcg cgcgtcgctc
    45001 acctggcgtc attttataat cgttcattac cacctctgtt ttaaattcgc gactagtgta
    45061 aagcggcaaa cgaatggggt aaacatgcgg attttttagt aaatatttcg agagggatat
    45121 gattctaatg ctaagaaaaa aggtgccgta gcaccttttt aactgagagg ttgtgttacc
    45181 acacagcagc cagcagcgta tgcgagtccg gcaccataaa atcaacggac atcatcaccg
    45241 agagggcagt gatggcgatg atagagaagc cgaacagctt gcgcgcccag attctgtcat
    45301 cagcaacttt ataaccgcgc agagccatgc ctaaccacca gacgctcacc gccgcggcga
    45361 ctaccagata tttataccca gcgtaaccgc caagagagag catcagcgtg gcaacggcaa
    45421 aggcgatgat atacagcgtg atgtgattct tcgccaccga aatgcctttt accactggca
    45481 ataccggaat gtttgccgcc tggtaatcct taaagcggaa aatggcgatg gcataggagt
    45541 gaggcatctg ccacagactg aagatagcaa gcaggatcgc tgcgccacta tcgaactcgc
    45601 cggttactgc acagtagccg atcaccggcg gcgcagcgcc ggagagcgaa ccaatcaatg
    45661 tgccgtagac tgagtggcgt ttcatgtaca ggctataaac gccgacataa accacaaagc
    45721 ccatcacccc cagccagcag gccagcggat tcgcgccaaa ccacagcagc ataaagccag
    45781 caatacccag caaggtggcg tacaccagcg agacagcagg tgagatcagg cctttaacca
    45841 gcacccgatt cttcgtcctt tccatctttc tgtcgatatc cctgtcgatg tagttgttaa
    45901 acacacaacc cgacgccaca accagtgaca ccccaaccag cgtgtagata aacaggggat
    45961 aatcaatgct gccctttgag gccaacagga atcccccaat caccgagatc aggttgccaa
    46021 agatgatgcc tggtttcgtt acttgcaggt attgcttaaa catcataacc gccgctctta
    46081 gtgcatcatc atgttgtagt tgaggttcca cataatccag atggagccta caaccaggat
    46141 agcgatgatt agcacggtga agacaaacgc cgtcatgttc cagccttcat ctgatttggt
    46201 attcatgtgc aggaagcaca ccagatgcac cagaacctgt accactgcca ttgccaggat
    46261 tgttcccaga attacggccg gagaggcagc tcctgtcatc accatccaga acggaatcac
    46321 cgtcagaatg atcgacagga taaagcctgt catgtaggtt tttacgctgc catgggacgc
    46381 gccgctgtga tcggtagaat gactcattac atcgccccca tcagataaac aacagtgaat
    46441 acacagatcc aaaccacatc caggaagtgc cagaacaggc tcaggcacat gatgcgggta
    46501 cgattagtgc tggtcaggcc gcgacgggcg atttgcacca tcagcaccgc catccagata
    46561 agaccggaag tgacgtgcag accgtgcgtg ccgaccagcg caaagaacgc tgacaggaag
    46621 ccgctgcgat ccggacccat gccgttaaca atcagatgat ggaattcata gagctccatc
    46681 ccgataaatc cggcgccaaa caaccaggtc aacgccagcc aggagacaac ctggcttttg
    46741 ttgtttttgt acatggcgat agccgccatg ccgtaggtga tggagctgaa caacagcagg
    46801 aaagtttcaa ccagaacgaa cggcagttcg aaaatgtcct tacctgtcgg gccgcctgcg
    46861 gtgccgttca ccagaacggc ataggtagca aacaggatag agaacagaat gcagtcgctc
    46921 atcaggtaga tccagaatcc gaagactttg gttccgcctg catcgtggtg cccgtgttcg
    46981 tgcgcgtggg cagtcgcgtg cgtcaaagta tcagttgcca tttttcagcc ctgccttagt
    47041 aatctcatcg aaatgctggt tttccagttt ttcgatttct gccaccggca cgtagtaatc
    47101 cacgtcctcg tcgaagcttt tcacgatcca ggtgatgatc atgcctgcga agccaacaat
    47161 cgccagccac cagatatgcc agatcatggc gaaaccgaag atggtggaga aagccgcaat
    47221 gacgatacct gcaccgctgt ttttcggcat atgaatttct tcatagtggt caggcttttt
    47281 gtacgcttcg cctttctctt tcatttccca gaatgcatca cgctcgtgaa cgtgcggcac
    47341 aacggcaaag ttatagaacg gaggcgggga agaggttgcc cactccagcg tacggccacc
    47401 ccacgggtcg ccagtcaggt cacggttctg gtcgcggtca cgaatagaaa cgtacatctg
    47461 aataacgagg cagagaatac ccagcgcaat cagtactgca ccgctggctg caatcatcag
    47521 catggtgtgg aactgcgggt caatctgctg gctcaaacga cgggtcatgc ccatgaagcc
    47581 cagcgcatac agcggcataa aggcaacgaa gaagccgatg atccagaacc agaacgcgcg
    47641 tttaccccag gtttcgttca gtttgaaacc gaacgcttta ggccaccagt aggtcatccc
    47701 tgcgaagcag ccgaagacca cgccgccgat gatcacgtta tggaagtgcg caatcaggaa
    47761 caggctgtta tgcagaacga agtccgcgcc cggtactgcc agcagcacgc cagtcatacc
    47821 gcctaccgag aaggtgacga taaaaccgat ggtccacagc atcgcagaat ggaacacgat
    47881 gcggccctga tacatggtga acagccagtt gaagatcttc accccggtcg ggatggcgat
    47941 aatcatagtg gtgataccaa agaaggcgtt tacgttcgcg cccgcaccca tcgtaaagaa
    48001 gtggtgcagc cagacgatga acgacagcac ggtgatacag acggttgccc ataccagcga
    48061 ggtataacca aacagacgtt tacgcgagaa ggttgccgca atttcggaga acacaccgaa
    48121 aacaggcagg atcaggatgt aaacttccgg gtggccccag gcccaaatca ggttgatgta
    48181 catcatcatg ttgccaccca tatcgttggt aaagaaatgg gtgcccagat agcgatccag
    48241 ggtcaacaac gcaacggtaa ccgtcagaat tgggaaggaa gcaataatca gtacgttcgc
    48301 gcacagtgat gcccaggtaa atactggcat cttgaacatg gtcatgcccg gtgcgcgcat
    48361 cttcagaatg gtaacgaaga agttgatacc ggtaagcgtt gtacctatac cggatagctg
    48421 gagactccat atccagtaat cgacaccgac tcccggactg tactctattc ccgatagcgg
    48481 tggataggcc agccagccgg tctgcgcaaa ttcgcccacg ccgagagaaa cgttaaccag
    48541 aatcacacca acaacggtaa accagaagct taggttgttg aggaacggga acgcaacgtc
    48601 acgcgcgccg atctgcagcg gaaccaccag gttcatcaga ccgataacga aaggcatcgc
    48661 cacgaagaag atcataatca cgccgtgcgc ggtgaagatc tgatcgtagt ggtgaggtgg
    48721 caggaagccc gcttcgcccg ccgaggcaag agcctgctgg ctacgcatca taatggcgtc
    48781 agcaaaacca cgcagcaaca tcacaatcgc cacgatgata tacatgatac cgaggcgttt
    48841 atggtcgacg gaggtcagcc actctttcca caggtaggtc cacttaccga agtaagtgat
    48901 caggccaacg agcgccagac ctcccaaaat aatgccagcg atcgtaacca tgacgatagg
    48961 ttcatggaac gggactgcat caagtgataa ttttccgaac atctttattc ttcctcaacc
    49021 cctttaatgg gcggattccg cgtggctcat gtccatgcct tccatacctt catgtgcgct
    49081 gtgctcacct tctggctggg tcatgtccat gctcttaccg tgagccataa acttgttaat
    49141 cacatcggca aacaagtctg gtttcacgtt ggagaaatat tccacctggt tgtattcgct
    49201 aggcgcggcc agtttttcga acgctgccat gtcagacatg ctgttcggcg actgtttcgc
    49261 ttttgcgacc cattggtcga atgcggcgcg atccggtgtt gcaatagctt tgaacttcat
    49321 gcctgagaag cccgggccgc tgtagctggc ggagataccg tcataagtac cgggttcgtt
    49381 ggcgatcaga tgcaggcgag tctgcatacc ggccatggca taaatctggc tacccagacg
    49441 cggaatgaag aaggagttca tcacggagtt ggaggtcact ttgaagtaca ccggagtgtt
    49501 cgccgggaaa gcgatttcat tcacggttgc aatgccctgt tccgggtaga tgaagaacca
    49561 tttccagtcc atggaaacca cttcgatggt aatgggcttc tcgtcgtgtg ccagcggctt
    49621 gctaggctca agagcgtgag tggttttcca ggtcagtacc gcaaggaaga tgatgattaa
    49681 gataggtacc gtccagacca cagcttccac tttattggag tgtgaccagt tcgggctgta
    49741 cttagcatct ttattgctcg cacggtactt ccaggcgaaa ccaacagcca tcaagattgc
    49801 gggaataacg acaatcaaca tcaggccaaa tgccgtcagt atcagtgaac gttgctccag
    49861 accaatctgt cctttgggat ctaacagcgc agaattacag ccactgagca atacagtgcc
    49921 tgcaaataat gacaaccatc ccaaactttt attgtatttc ctgagtctca tttaacgacc
    49981 tcaattccac gggatctggt ggcgtttaaa gtgtgtgggc attttacggg aaggttacat
    50041 tactgtaaac atgattaaat tcctgttact tggatttggc ttacaggtca catatgttgc
    50101 aaaacatatc aggttgattt taagaagctg ttgcaaaagg gcgttataac aaaaggggag
    50161 tgcagaagga gtctgggcgg gcaattggta taaccaatgc gaaataaaac aattatttaa
    50221 caaataatta tcattaaggt gacaactggg tagtgggaaa ataaagactc attagctgaa
    50281 tcgtgtaaga aaaatagaat tcataaatgg cgtcaataat ttccagacgc taagccgcac
    50341 aaaagaacaa atattaataa gcgatgctga tgtttattta gccgtaataa ttacggcgag
    50401 tgattactac ggctaaataa tatttacaga ttacgtcaga tgcgtttttc gtagcgccag
    50461 ataatccagt aaaccaccaa gcacgacgcc agaaagggcg actaaaaccc cgacttccag
    50521 cagagcaggc aggaaagaga agtgcgtcaa atccagcgcg tccatcgtca acagcagtaa
    50581 ccacacggcc aacaggctga cgcccgccgc cagtgtccac atggcaaagg cataacctgc
    50641 cggatattcg gtacgggaga taaagttatc atttactcgt gtatattcaa gcgtctggcg
    50701 acaaaccagc agcaagataa gccctggtac ggcagcggcg acggagaata gatagaatgt
    50761 cgaccagccg tgtgcttcaa caaaccaacc cgccacgggg ccgacataaa ctcgccctac
    50821 agcagaaagc gctgagagca gggcaaattg agtagcggaa aatgacttat tacacagcgt
    50881 cattaacagc gcgacaaagg ctgatgtacc catcccgcca cagaggtttt cgaaaaagac
    50941 ggctgcgccc atgctgtaga gatgcttatc agtaatcgac agcagccagt aaccggcgtt
    51001 agatgcacct tgtaaaatgc cgaaaatcag cagtgcacgg aacagcgaca ggcgctgcat
    51061 caaaatccca ccgtacaatg cgccaacaat ggtcgccagt aagccaagcg ttttgttaac
    51121 cacgcctact tcacccgcat caaacccgac gccgcgaatc aaaaacgtgg ttgtcaggct
    51181 catagcgaat gcgtcgccca gcttatacag cacgataaga agcaaaataa gccaggcatt
    51241 attgcgacca aagaaatctc gcagaggtgc aacaaccgct tgttccagcg ttttgggcac
    51301 aggaatggtg tcggtgggtt ctggtgcaag caacgtcgcg ataatacagg ggatcaacag
    51361 cgccgccatc aaccagtaca tgccctgcca gcccagccat ttatctgcca gccacagggc
    51421 taagccgccg gaaaccagca tccctaaacg gtaacccagc acgctgattg ccgcgcccgc
    51481 accacgttct tctgccggga gcacatcggt tttccacgca tcgaagacaa tatcctggga
    51541 ggcagagcaa aaagcgatca ccaccgccag tgccgccatc cagcggagct gggtgcctgg
    51601 ttcgagaaaa cccatcgccg caatggcgac taataacaga atttgcgtgg cgagcagcca
    51661 accgcgccgc cgcccgaaaa atgggggcgt gtagcggtcc atcagtggtg accagaggaa
    51721 tttaaaaacg taagcctggc ctaccagaga gaagaaacca atggttttga gatcgatatt
    51781 ctcgaccgtc atccaggcct gtaaggtgcc ggacgtcagg gcgagcggta gcccggaagc
    51841 aaaacccagg atcagcaata tggctgaacg cggctgttga aaaatacgta aatattgact
    51901 ggacatgggc gtctacaggc ccggcttgca ccgggccaga gggcagaaat taacgcgcgt
    51961 tctgcttgat gaattcgtgg atgctggtgt cctgagacat atcagcgatg gtatcggtca
    52021 gcacactgtt aaccgcatcg gcgatatttt tgttggaggc ctggaacgca ccttcaacgt
    52081 tgtagctggc acgatagttt ttggtcattt tgttgccatt ctgcgcggta gcgatgatgg
    52141 cgatatccgc tttggtcgcg atgttgtagc gcacgttgcc ctgggacacg tcagcataca
    52201 gttggctaac gatgatttgc agattaaccg ggccattcgg accaaccatg tatccacggg
    52261 cggtcatttg tttttccagc acttcttgca gcaggaaacg cagatcgcgg gaggcggtca
    52321 gggtaacgat ttgattatcg cgggtgactt ttgccagcgc ctgatcggta cgctgatcgg
    52381 caccattaat gcttacggtg acgcccatca ggcttggatc ctgctgtggc agtgtaatcg
    52441 tcggggaaac ttcaatagtt gttggcggtt ttgcgcatcc tgcaagcata aacagagcaa
    52501 ctaacgggaa gaggattttt ttgaacatgt tcgggctctc agagactctt aagcgtgttt
    52561 ggtaaaaatt cccgccatca taacattgcc aacggcgagg ggaagtgggt aaggcatgta
    52621 aattcatcat gttgacgaaa taatcgcccc tggtaaaaga aacgctgatg cgaggcctgt
    52681 gtttcaatct ttaaatcagt aaacttcata cgcttgacag aaaaaccagg acgaaaccta
    52741 aatatttgtt gttaagctgc aatggaaacg gtaaaagcgg ctagtattta aagggatgga
    52801 tgacatctca gcgttgtcgg aggagatatt tcatgatgat acgtgagcgg atagaagaaa
    52861 aattaagggc ggcgttccaa cccgtattcc tcgaagtagt ggatgaaagc tatcgtcaca
    52921 atgtcccagc cggctctgaa agccatttta aagttgtgct ggtcagcgat cgttttacgg
    52981 gtgaacgttt tctgaatcgt catcgaatga tttacagtac tttagcggag gaactctcta
    53041 ctaccgttca tgcgctggct ctgcatactt acactattaa ggagtgggaa gggttgcagg
    53101 acaccgtctt tgcctctcct ccctgtcgtg gagcaggaag catcgcgtaa aaacgcattt
    53161 gcaactgtcg gcgcttttcc agtatgttgc taaagatttt atgaaaaacg gcctgcgggc
    53221 cgttttgttt tgtctggatt ttgcgctttt tgcccagcat tcagacgaaa attgcccggg
    53281 aattgtgaaa aaatacgcga cagcgcgcaa taaccgttct cgactcataa aagtgatgcc
    53341 gctataatgc cgcgtcctat ttgaatgctt tcgggatgat tctggtaaca gggaatgtga
    53401 ttgattataa gaacatcccg gttccgcgaa gccaacaacc tgtgcttgcg gggtaagagt
    53461 tgaccgagca ctgtgatttt ttgaggtaac aagatgcaag tttcagttga aaccactcaa
    53521 ggccttggcc gccgtgtaac gattactatc gctgctgaca gcatcgagac cgctgttaaa
    53581 agcgagctgg tcaacgttgc gaaaaaagta cgtattgacg gcttccgcaa aggcaaagtg
    53641 ccaatgaata tcgttgctca gcgttatggt gcgtctgtac gccaggacgt tctgggtgac
    53701 ctgatgagcc gtaacttcat tgacgccatc attaaagaaa aaatcaatcc ggctggcgca
    53761 ccgacttatg ttccgggcga atacaagctg ggtgaagact tcacctactc tgtagagttt
    53821 gaagtttatc cggaagttga actgcagggt ctggaagcga tcgaagttga aaaaccgatc
    53881 gttgaagtga ccgacgctga cgttgacggc atgctggata ctctgcgtaa acagcaggcg
    53941 acctggaaag aaaaagacgg cgctgttgaa gcagaagacc gcgtaaccat cgacttcacc
    54001 ggttctgtag acggcgaaga gttcgaaggc ggtaaagcgt ctgatttcgt actggcgatg
    54061 ggccaggggc gtatgattcc gggctttgaa gacggtatca aaggccacaa agctggcgaa
    54121 gagttcacca tcgacgtgac cttcccggaa gaataccacg cagaaaacct gaaaggtaaa
    54181 gcagcgaaat tcgctatcaa cctgaagaaa gttgaagagc gtgaactgcc ggaactgact
    54241 gcagaattca tcaaacgttt cggcgttgaa gatggttccg tagaaggtct gcgcgctgaa
    54301 gtgcgtaaaa acatggagcg cgagctgaag agcgccatcc gtaaccgcgt taagtctcag
    54361 gcgatcgaag gtctggtaaa agctaacgac atcgacgtac cggctgcgct gatcgacagc
    54421 gaaatcgacg ttctgcgtcg ccaggctgca cagcgtttcg gtggcaacga aaaacaagct
    54481 ctggaactgc cgcgtgaact gtttgaagaa caggctaaac gtcgcgtagt tgttggcctg
    54541 ctgctgggcg aagttatccg caccaacgag ctgaaagctg acgaagagcg cgtgaaaggc
    54601 ctgatcgaag agatggcttc tgcgtacgaa gatccgaaag aagttatcga gttctacagc
    54661 aaaaacaaag aactgatgga caacatgcgc aatgttgctc tggaagaaca ggctgttgaa
    54721 gctgtactgg cgaaagcgaa agtgactgaa aaagaaacca ctttcaacga gctgatgaac
    54781 cagcaggcgt aatttacgca gcataacgcg ctaaattcgc acaaaggccc gtcaccgcca
    54841 ggtggtgggc ttttttttgt catgaatttt gcatggaacc gtgcgaaaag cctctttcgg
    54901 tgttagcgta acaacaaaag attgttatgc ttgaaatatg gtgatgccgt acccataaca
    54961 cagggactag ctgataatcc gtccataagg ttacaatcgg tacagcaggt tttttcaatt
    55021 ttatccagga gacggaaatg tcatacagcg gcgaacgaga taactttgca ccccatatgg
    55081 cgctggtgcc gatggtcatt gaacagacct cacgtggtga gcgctctttt gatatctatt
    55141 ctcgtctact taaggaacgc gtcatttttc tgactggcca ggttgaagac cacatggcta
    55201 acctgattgt ggcgcagatg ctgttcctgg aagcagaaaa cccagaaaaa gatatctatc
    55261 tgtacattaa ctccccaggc ggggtgatca ctgccgggat gtctatctat gacaccatgc
    55321 agtttatcaa gcctgatgtc agcaccatct gtatgggcca ggcggcttcg atgggcgctt
    55381 tcttgctgac tgcaggggca aaaggtaaac gtttctgcct gccgaattcg cgcgtgatga
    55441 ttcaccagcc gttgggcggc taccagggcc aggcgaccga tatcgaaatt catgcccgtg
    55501 aaattctgaa agttaaaggg cgcatgaatg aacttatggc gcttcatacg ggtcaatcat
    55561 tagaacagat tgaacgtgat accgagcgcg atcgcttcct ttccgcccct gaagcggtgg
    55621 aatacggtct ggtcgattcg attctgaccc atcgtaattg atgccagagg cgcaactgtg
    55681 ccgctatact tatccagggc ggcacaacgc tgtaagcggc ttgcgcctga gaatggcatt
    55741 tgcgtcgtcg tgtgcggcac aaagaacaaa gaagaggttt tgacccatga cagataaacg
    55801 caaagatggc tcaggcaaat tgctgtattg ctctttttgc ggcaaaagcc agcatgaagt
    55861 gcgcaagctg attgccggtc catccgtgta tatctgcgac gaatgtgttg atttatgtaa
    55921 cgacatcatt cgcgaagaga ttaaagaagt tgcaccgcat cgtgaacgca gtgcgctacc
    55981 gacgccgcat gaaattcgca accacctgga cgattacgtt atcggccagg aacaggcgaa
    56041 aaaagtgctg gcggtcgcgg tatacaacca ctacaaacgt ctgcgcaacg gcgataccag
    56101 caatggtgtc gagttgggca aaagtaacat tctgctgatc ggtccgaccg gttccggtaa
    56161 aacgctgctg gctgaaacgc tggcgcgcct gctggacgtc ccgttcacca tggccgacgc
    56221 aaccacgctg accgaagccg gttatgtggg cgaagacgtt gaaaacatca ttcagaagct
    56281 gttgcagaag tgcgattacg acgtacagaa agcgcagcgc gggattgtct acatcgatga
    56341 aatcgacaag atttctcgta agtcagacaa cccgtccatt acccgagacg tttccggtga
    56401 aggcgtacag caggcactgt tgaaactgat cgaaggtacg gtagctgctg ttccaccgca
    56461 aggtgggcgt aaacatccgc agcaggaatt cttgcaggtt gatacctcta agatcctgtt
    56521 tatttgtggc ggtgcgtttg ccggtctgga taaagtgatt tcccatcgtg tagaaaccgg
    56581 ctccggcatt ggttttggcg cgacggtaaa agcgaagtcc gacaaagcaa gcgaaggcga
    56641 gctgctggcg caggttgaac cggaagatct gatcaagttt ggtcttatcc ctgagtttat
    56701 tggtcgtctg ccggttgtcg caacgttgaa tgaactgagc gaagaagctc tgattcagat
    56761 cctcaaagag ccgaaaaacg ccctgaccaa gcagtatcag gcgctgttta atctggaagg
    56821 cgtggatctg gaattccgtg acgaggcgct ggatgctatc gctaagaaag cgatggcgcg
    56881 taaaaccggt gcccgtggcc tgcgttccat cgtagaagcc gcactgctcg ataccatgta
    56941 cgatctgccg tccatggaag acgtcgaaaa agtggttatc gacgaatcgg taattgatgg
    57001 tcaaagcaag ccgttgctga tttatggcaa gccggaagcg caacaggcat ctggtgaata
    57061 attaaccatt cccatacaat tagttaacca aaaagggggg attttatctc ccctttaatt
    57121 tttcctctat tctcggcgtt gaatgtgggg gaaacatccc catatactga cgtacatgtt
    57181 aatagatggc gtgaagcaca gtcgtgtcat ctgattacct ggcggaaatt aaactaagag
    57241 agagctctat gaatcctgag cgttctgaac gcattgaaat ccccgtattg ccgctgcgcg
    57301 atgtggtggt ttatccgcac atggtcatcc ccttatttgt cgggcgggaa aaatctatcc
    57361 gttgtctgga agcggcgatg gaccatgata aaaaaattat gctggtcgcg cagaaagaag
    57421 cttcaacgga tgagccgggt gtaaacgatc ttttcaccgt cgggaccgtg gcctctatat
    57481 tgcagatgct gaaactgcct gacggcaccg tcaaagtgct ggtcgagggg ttacagcgcg
    57541 cgcgtatttc tgcgctctct gacaatggcg aacacttttc tgcgaaggcg gagtatctgg
    57601 agtcgccgac cattgatgag cgggaacagg aagtgctggt gcgtactgca atcagccagt
    57661 tcgaaggcta catcaagctg aacaaaaaaa tcccaccaga agtgctgacg tcgctgaata
    57721 gcatcgacga cccggcgcgt ctggcggata ccattgctgc acatatgccg ctgaaactgg
    57781 ctgacaaaca gtccgttctg gagatgtccg acgttaacga acgtctggaa tatctgatgg
    57841 caatgatgga atcggaaatc gatctgctgc aggttgagaa acgcattcgc aaccgcgtta
    57901 aaaagcagat ggagaaatcc cagcgtgagt actatctgaa cgagcaaatg aaagctattc
    57961 agaaagaact cggtgaaatg gacgacgcgc cggacgaaaa cgaagccctg aagcgcaaaa
    58021 ttgacgcggc gaaaatgccg aaagaggcaa aagagaaagc ggaagcagag ttgcagaagc
    58081 tgaaaatgat gtctccgatg tcggcagaag cgaccgtagt gcgtggttat atcgactgga
    58141 tggtacaggt gccgtggaat gcgcgcagca aggtcaaaaa agacctgcgt caggcgcagg
    58201 aaatccttga taccgaccat tatggtctgg agcgcgtgaa agatcgcatc cttgagtatc
    58261 ttgcggttca aagccgtgtc aacaaaatca agggaccgat cctctgcctg gtagggccgc
    58321 cgggggtagg taaaacctct cttggtcagt ccattgccaa agccaccggg cgtaaatatg
    58381 tccgtatggc gctgggcggc gtgcgtgatg aagcggaaat ccgtggtcac cgccgtactt
    58441 acatcggttc tatgccgggt aaactgatcc agaaaatggc gaaagtgggc gtgaaaaacc
    58501 cgctgttcct gctcgatgag atcgacaaaa tgtcttctga catgcgtggc gatccggcct
    58561 ccgcactgct tgaagtgctg gatccagagc agaacgtagc gttcagcgac cactacctgg
    58621 aagtggatta cgaccttagc gacgtgatgt ttgtcgcgac gtcgaactcc atgaacattc
    58681 cggcaccgct gctggatcgt atggaagtga ttcgcctctc cggttatacc gaagatgaaa
    58741 aactgaacat cgccaaacgt cacctgctgc cgaagcagat tgaacgtaat gcactgaaaa
    58801 aaggtgagct gaccgtcgac gatagcgcca ttatcggcat tattcgttac tacacccgtg
    58861 aggcgggcgt gcgtggtctg gagcgtgaaa tctccaaact gtgtcgcaaa gcggttaagc
    58921 agttactgct cgataagtca ttaaaacata tcgaaattaa cggcgataac ctgcatgact
    58981 atctcggtgt tcagcgcttc gactatggtc gcgcggataa cgaaaaccgt gtcggtcagg
    59041 taactggtct ggcgtggacg gaagtgggcg gtgacttgct gaccattgaa accgcgtgcg
    59101 ttccgggtaa aggcaaactg acctataccg gatcgcttgg cgaagtgatg caggagtcca
    59161 ttcaggcggc gttaacggtg gttcgtgcgc gtgcggaaaa actggggatc aaccctgatt
    59221 tttatgaaaa acgcgacatc cacgtccacg taccggaagg tgcgacgccg aaagatggtc
    59281 cgagtgccgg tattgctatg tgcaccgcgc tggtttcttg cctgaccggt aacccggttc
    59341 gtgctgatgt ggcaatgacc ggtgagatca ctctgcgtgg tcaggtactg cctatcggtg
    59401 gtttgaaaga aaaactactg gcagcgcatc gcggcgggat caaaacagtg ttaattccgt
    59461 tcgaaaataa acgcgatctg gaagagattc ctgacaacgt aattgccgat ctggatattc
    59521 atcctgtgaa acgcattgag gaagttctga ctctggcgct gcaaaatgaa ccgtctggca
    59581 tgcaggttgt gactgcaaaa tagtgacctc gcgcaaaatg cactaataaa aacagggctg
    59641 gcaggctaat tcggacttgc cagccttttt ttgtctcgct aagttagatg gcggattggg
    59701 cttgccctta ttaaggggtg ttgtaagggg atggctggcc tgatataact gctgcgcgtt
    59761 cgtaccttga aggattcaag tgcgatataa attataaaga ggaagagaag agtgaataaa
    59821 tctcaattga tcgacaagat tgctgcaggg gctgatatct ctaaagctgc ggctggccgt
    59881 gcgttagatg ctattattgc ttccgtaact gaatctctga aagaagggga tgatgtagca
    59941 ctggtaggtt ttggtacttt tgccgttaaa gagcgtgctg cccgtactgg ccgcaatccg
    60001 cagaccggta aagagatcac catcgctgct gctaaagtac cgagcttccg tgcaggtaaa
    60061 gcactgaaag acgcggtaaa ctaagcgttg tccccagtgg ggatgtgacg aagttcaagg
    60121 gcgcatctac tgatgtgcct tttttatttg tattcggtga ctttctgcgt cttgtgggct
    60181 gacaattgcc cccgtttctt gtcacaatat gcctttgcgc gcatcgatac gttgcgtgag
    60241 gtacacagtc atctacagcg gagtgttgtt acaccatgat ggacagctta cgcacggctg
    60301 caaacagtct cgtgctcaag attattttcg gtatcattat cgtgtcgttc atattgaccg
    60361 gcgtgagtgg ttacctgatt ggcggaggca ataactacgc cgcaaaagtg aatgaccagg
    60421 aaatcagtcg tgggcaattc gagaacgcct tcaacagcga gcgtaatcgc atgcagcaac
    60481 agctgggcga tcaatactcc gagctggcag cgaacgaagg ctatatgaaa accctgcgtc
    60541 aacaggtgct gaatcgtctg atcgacgagg cgctgctgga tcagtacgct cgtgagctga
    60601 aactgggtat cagcgatgag caggttaaac aggcgatttt cgcgacccca gccttccagg
    60661 ttgacggcaa atttgataac agccgctata acggtatcct caaccagatg gggatgaccg
    60721 ccgatcagta cgcccaggcg ctgcgtaacc agctcactac ccaacagctg attaacggcg
    60781 ttgccggtac cgattttatg ctgaaaggtg aaaccgacga gctggcggca ctggtagttc
    60841 aacaacgcgt ggtgcgtgaa gcgactatcg atgttaacgc gctggcggcg aagcagcctg
    60901 tgaccgaaca ggaaattgcc agctactacg aacaaaacaa aaacaatttc atgacgccgg
    60961 aacaattccg cgtgagttac atcaagctgg atgccgcaac gatgcagcaa ccggttagcg
    61021 atgcggatat ccagagctac tacgatcagc atcaggatca attcacccag ccgcagcgta
    61081 cccgttacag catcatccag accaaaactg aagatgaagc gaaagcggta cttgatgagc
    61141 tgaataaggg cggtgatttt gctgcattag ccaaagaaaa atctgccgat attatctctg
    61201 cccgcaacgg cggtgatatg ggttggttag aagatgccac tatcccggac gaactgaaaa
    61261 atgctggtct gaaaaaaaaa ggccaactgt ctggtgttat caaatcttcg gtcggtttcc
    61321 tgattgtgcg tctggacgac attcagccag cgaaagtgaa atcgttagac gaagtacgtg
    61381 acgacattgc ggcgaaagtg aaacacgaaa aagccctcga tgcgtactac gcgctgcagc
    61441 agaaagtgag cgatgcggca agcaacgaca ccgagtctct ggccggtgca gagcaagctg
    61501 ccggcgttaa agccactcag acgggttggt tcagcaaaga taacctgccg gaagagttga
    61561 acttcaagcc agttgccgac gctatcttca acggcggtct ggtcggtgaa aacggcgcgc
    61621 cgggcatcaa ctctgacatc atcaccgtag acggcgaccg cgcattcgtg ctgcgcatca
    61681 gcgagcacaa accggaagcg gtgaaaccgt tggcagatgt tcaggaacaa gttaaggcac
    61741 tggttcagca caacaaagct gaacaacagg cgaaagtgga tgctgagaaa ctgctggttg
    61801 atttgaaagc aggcaaaggt gcggaagcta tgcaggctgc cggtctgaaa tttggcgagc
    61861 cgaaaacctt aagtcgttcc ggtcgtgacc cgattagcca ggctgcgttt gcactgccgc
    61921 tgccagcgaa agacaaaccg agctacggta tggcgaccga tatgcaaggt aatgtagttc
    61981 tgctggcgct ggatgaagtg aagcaaggtt caatgccgga agatcagaaa aaagcgatgg
    62041 tgcagggtat cacccagaac aacgcacaaa tcgtctttga agctctgatg agtaacctgc
    62101 gtaaagaggc gaaaatcaaa attggcgatg cgctggaaca gcaataatcc tgaagccgcc
    62161 tcgcaaaaaa aatgctttgc agctgtaaca agaaaaggtc gctttcgcgg ccttttccat
    62221 ttctgaacat tgccatttgt ttactgtttt cactgccgtt aaggtgattc cactgttaac
    62281 aaacaaggag aaaacagtat gaaacacgga attaaagcac tgctcattac cctgtccctg
    62341 gcctgtgccg gaatgtctca tagtgctctg gcggcagctc ctgcggcaaa accgacaacg
    62401 gtagaaacca aagcggaagc tcctgcagca caaagtaaag cagcagttcc ggcgaaagcc
    62461 agtgacgaag aaggcacccg ggtcagcatt aataatgcca gcgcggaaga gttagcccgc
    62521 gcgatgaatg gcgttggcct gaagaaagcg caggcgattg tcagttatcg cgaagagtac
    62581 ggtccgttta aaacggtgga ggatctaaag caggtgccgg ggatgggcaa ttcgctggtg
    62641 gagcgtaatc tggcggtatt aaccctgtaa ttaatttgca tagtggcaat tttttgccag
    62701 actgaagagg tcataccagt tatgacctct gtacttataa caacaacgta aggttctcgc
    62761 gctatgcaaa cacaaatcaa agttcgtgga tatcatctcg atgtttacca gcacgttaat
    62821 aacgcccgct atctggagtt tctcgaagaa gcccgctggg atgggttgga aaatagcgac
    62881 agttttcagt ggatgacggc ccataacatc gctttcgtgg tggtgaatat caatattaac
    62941 tatcgtcgcc cggcggtatt aagcgacttg ttaaccatta cgagccagtt acagcaatta
    63001 aacggtaaaa gcggcatctt aagccaggtc attacactgg agccggaagg gcaggtggta
    63061 gcggatgcgc ttattacgtt tgtttgtatt gatcttaaaa cgcagaaagc attagctctg
    63121 gaaggggaat tgcgcgaaaa gctcgagcag atggttaagt aaacgttttg tggtgccgga
    63181 tgctcaagcc gcatccggcg acacccggaa taattacttc aacccggttt tctgcttcat
    63241 cgctgccatc accgtcggtt tatcggccag ataatgatgc aaaccgttgg cacgtaaatt
    63301 acacgccgca caatgaccgc aaccgtcgcc tttaatgccg ttataacagg tcagcgtttc
    63361 gttacggact aaatccagct tgccgtaata atctgccagc gcccaggttt ccgctttatc
    63421 aatccacatc agcggcgttt caaaacgaat gtctttcgcc atacccagac tgacggcatg
    63481 gttcagtgct ttcacaaact catcgcggca atccgggtag ccggagaaat ccgtttcgca
    63541 gacgccggta atcaccgctt ctgcttttac ctgatacgca tatattgccg ccagtgtcag
    63601 gaacaaaata ttacgcccag ggacaaacgt attcgggatg ccatcggctt caggttcata
    63661 atctggcact ggaatgctgt cacgtgtcag gctgctgacc gccagctcgt tgagcagcgt
    63721 tacatcaaga accttatgcg cgcgtgcccc cagtttcagc gccagttcgc gtgccacgtc
    63781 gatttccgcg cgatgtcgct gaccgtaatc gaacgtcacg caatggactt catcatattg
    63841 ttgtaatgcc tgcaccagac aggtggtaga atcctggcct ccactgaaca caacgacagc
    63901 acgtttcata gataatcctg cttgaacaat aaaagcgtta tggtaacgcc tgcgatgaac
    63961 ccggaccagc tattcattgc gatggcgcgg gaagccaggc ttcggtaaat tcaaaccagc
    64021 ctcgcggtgt cagtcgcaca ccgttcacgc cgggaggggc gctaatgcga tagtgatagt
    64081 tgaacagcgg cgtcagcgtc gcatctgcca ttaactggct aaaaaccgct ttcagggcat
    64141 taaatcggtt ttcctcatca ggcattactt gcaccgcgtc cagtgtcgat tgcaaatgtg
    64201 catatgctgg agcgtcgaaa acatgtggcc atagtggatc gcaacgcagc cattgctcca
    64261 gggtatattc cggtgcttcg ccaattaatc tgtcgcccat catgaggtct gcgtgtgcca
    64321 gtagggtcgt gtcgtcccag ttttttgcgt tatgaaaaat aattgtgagt tcacagcctt
    64381 ccgctgccag tgtcgcctgt agacgttctg ccatggtatg aagttctatc ggtaggtgat
    64441 aaaccagcgt caaggttttc ggtagtttga cctcatccgg tacttgccat tgcggaatag
    64501 tccagcctgg cagtaatgca tgactggcgg tgatcaggtt ttctccgact tctaacgttt
    64561 gtaataaacc ggattgatga ataatggaaa tcacttttcg cgcctgccag agggacagtc
    64621 ggggactttt gcgcaacgtt aaatagcaaa aacctaaact gatgccacta ctgacctggc
    64681 tgacccgttg cagctcctcc ggtttgccga tggtgatttg cacgggatgc cgacaactgg
    64741 ttcccaaatc tttttcgaaa agcggcggag ttatccagta ctcaaccgct ttaagcagcg
    64801 gatgacgtaa atggtaataa tcatggcttt ccaggcgcac cagctccgct gtgaattgtg
    64861 ttaagcgaaa aggacccgtg ccgatcagtg ggaattgcgg atgcgccaga tggctgcaat
    64921 agctcgccag tcggtgcgca agccagtaat cggggcgatg taaaaagaag gtcagacact
    64981 gcggatgggt gacttcaata cgcttcacgc taataaataa ttgatcccgt gctggcagtt
    65041 gtaacagcag caataatcgc tggtgtaagt gtgaggcttt tactgcatcg ccgttatgcc
    65101 agtgtagggt tgaacgaaga taaaagtccc agcgtaaccc gtcagtagag gtttcccagt
    65161 gatgtgctaa atcgccaatc gggcgctgag tattattatc gaagcgggtc aggccggaaa
    65221 atatctgccc ggccagatgc tgctcggcac ggccgggcaa aaagcccggt tgtagcggtt
    65281 cgagcgggcg atagtaggga atacgcaacg tgggcgtatc gttttgccat tgtccgccca
    65341 taaacggctg taacagagtg cgcagctcac ctggggccag ttgcgccagc tccagcacat
    65401 cttgctgctt tccggtttcc agcgcctgtt ccatcatcgc attgcgtagc gattctggcg
    65461 tgaccagaaa gcgtagttgc ccgcgttttc cgcgtcctga ctgcgcctgc cactccagcc
    65521 atcccgcctc ctgtgcctgg cgtaacagcg tacgaacatg gcgttcgctg caaaaacagc
    65581 gttcggccag ttcgctgacg gtgacggttt gcggctttcc ggcggaaggt tgccacagac
    65641 gttgatactg gttaagacgg ttgagcagtc gcatataaac ccggaacaat attatttaac
    65701 tattcactat tacttccgta tatatcaggt gatactcaat caccattaac cgtgtcacag
    65761 agtggagaaa gaaatggctc gtctggcagc atttgatatg gatggcactt tattgatgcc
    65821 tgaccatcat ttaggtgaga aaaccctctc tactttggcg cgactgcgtg aacgcgacat
    65881 taccctcact tttgccactg ggcgtcatgc gctggagatg cagcatattc tcggggcgct
    65941 atcgctggat gcatatttga ttaccggcaa cggaacgcgc gtgcattctc tggaaggtga
    66001 acttttacat cgtgatgatt tacctgcgga tgtcgcggag ctggtgctgt atcagcagtg
    66061 ggatacccga gccagcatgc atatcttcaa tgacgacggt tggtttaccg ggaaagagat
    66121 ccctgcgttg ttgcaggcat ttgtctatag tggttttcgt tatcagataa tcgatgtcaa
    66181 aaaaatgcca ctcggcagcg tcaccaagat ctgcttctgt ggcgatcacg acgatcttac
    66241 acgcttgcag atccagctat acgaagcatt aggcgagcgt gcacatttgt gtttttccgc
    66301 cacggattgc ctcgaagtgc tgccggtggg ctgcaataaa ggcgctgcat tgacggtgct
    66361 gacccaacat ttaggtttat cgttgcgcga ttgcatggcc tttggtgatg cgatgaacga
    66421 tcgcgaaatg ttaggcaacg tcggtagcgg atttattatg ggcaatgcga tgccgcaatt
    66481 gcgcgcggag ctcccgcatt taccggtgat tggacattgc cgaaatcagg ctgtctctca
    66541 ctatttgacg cactggctgg actatccaca tctaccttat tcccccgaat aacgagatcc
    66601 cttccagcac cgggcaattg cccggttttt tttgcgttga atttgtcatt ttgtgccgtg
    66661 gtgtttaaac cgcacagaat aaattgtcgc gatttcacct ttaaaataga attaaaagag
    66721 aaaaaaattc tctgtggaag ggctatgtta gataaaattg accgtaagct gctggcctta
    66781 ctacagcagg attgtaccct ctctttgcag gcactggctg aagccgttaa tctgacaacc
    66841 accccgtgct ggaagcgtct gaaacggctg gaggacgacg gtattcttat cggcaaagtc
    66901 gccctgctgg atccggaaaa aataggcctc ggcctgaccg cctttgtgct gataaaaacg
    66961 caacatcaca gcagcgaatg gtattgccgc tttgtcacgg tggttaccga aatgccagaa
    67021 gtgctggggt tctggcgcat ggccggtgaa tacgattatc tgatgcgcgt ccaggttgcc
    67081 gacatgaaac gctacgacga gttttataag cgtttggtaa acagcgtgcc ggggctgtcg
    67141 gacgtcactt ccagcttcgc gatggaacag attaaataca ccacttcttt acccatcgaa
    67201 taaatatcca gaatcaggtc aggacacaac gcgtgcgatt atttgctcaa ttaagctggt
    67261 atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc cttgcttgtc attatcgcga
    67321 tgctgcaact ggttccgcca aaagtggttg gtattgttgt tgatggcgtg acagaacaac
    67381 actttactac cgggcagatc ctgatgtgga tcgccaccat ggtgctgatt gccgttgtgg
    67441 tttatctcct gcgttacgtc tggcgtgtat tgctgtttgg cgcgtcttat caactggctg
    67501 ttgaactgcg tgaagattat taccgtcagc taagccggca gcatcctgag ttttacctgc
    67561 gtcatcgcac gggtgacctc atggctcgtg cgaccaatga cgtcgatcgc gtcgtgtttg
    67621 ccgccggaga aggggtgctg acgctggtgg attcactggt aatgggctgc gctgtgttga
    67681 ttatgatgtc tacgcaaatt agctggcagt tgaccttatt tgccctgttg ccgatgccag
    67741 tgatggcgat catgattaag cgcaacggcg atgccttgca tgaacgcttt aagctggcgc
    67801 aggcggcgtt ttccagtctt aatgaccgca cccaggaaag cctcaccagt atccgcatga
    67861 tcaaagcttt tggtctggaa gatcgccagt cggcgttatt tgccgaggat gccgaagata
    67921 ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg tttcgacccg accatctata
    67981 tcgcgattgg tatggcgaac ttgctggcga ttggcggcgg tagttggatg gtggtgcagg
    68041 gcagtttaac gctgggccag ctcaccagtt ttatgatgta tttaggtctg atgatttggc
    68101 caatgctggc gctggcatgg atgtttaaca tagtggaacg tggtagtgct gcgtacagcc
    68161 gcattcgcgc gatgctggcg gaagcgccgg tggtgaatga tggtagcgaa cccgtgccgg
    68221 aaggtcgtgg cgaactggat gtaaatattc gccagttcac gtatccgcag actgaccatc
    68281 ctgcgctgga aaacgtcaat ttcgccctga aacccggtca gatgctgggt atttgcgggc
    68341 cgactggttc cggcaaaagt accctgttgt cgctcattca gcgtcatttc gacgtcagcg
    68401 agggggatat ccgctttcat gatattcctc tgacgaagtt acaactcgat agctggcgta
    68461 gccgcctggc ggtagttagc cagacaccgt tccttttttc cgacaccgta gcgaacaaca
    68521 tcgcgttggg ttgcccgaat gccacccagc aagagattga gcatgtcgcg cggttagcca
    68581 gcgtgcatga cgatattttg cgtttaccgc aaggttacga tacagaagtg ggcgagcgcg
    68641 gcgtgatgct ttccggtggg caaaaacagc gtatctccat tgctcgtgcg ttattagtca
    68701 acgcggaaat cctcatcctt gatgatgcgc tttcggcagt agacggacgc acagagcacc
    68761 agatcctgca taacctgcgt cagtgggggc agggaagaac ggtaatcatc agcgcccatc
    68821 gcctttctgc actgacggaa gccagtgaaa ttattgtgat gcagcacgga catatcgctc
    68881 agcgtggcaa tcatgatgag ctggcgcaac aaagcggctg gtatcgcgat atgtatcgct
    68941 atcaacaact ggaggcggcg ctcgacgacg ctccggaaat tcgcgaggag gccatcgatg
    69001 cgtagtttta gccaactgtg gccgactctc aagcgtctgt tagcgtacgg ttcgccgtgg
    69061 cgtaaaccgc tggggattgc ggtcataatg atgtgggttg cggcagcggc agaagtcagt
    69121 gggccgctgc ttatcagcta ttttatcgac aatatggtag cgaaaaataa cctgccgttg
    69181 aaagtggttg cagggctggc tgcggcgtat gttgggctgc aactgtttgc cgccgggcta
    69241 cattacgcgc agtcgctgct gtttaatcgg gcggcagtag gcgtagtgca acagttgcgt
    69301 accgacgtga tggatgctgc gttacgccag ccattaagcg agtttgatac ccaacccgtc
    69361 gggcaggtga tttcccgcgt cactaatgac actgaagtga tccgcgacct ctacgttacc
    69421 gtagtggcaa ctgtcctgcg tagcgccgca ctggtgggcg cgatgctggt ggcgatgttc
    69481 agcctcgact ggcgaatggc gctggtggcg ataatgattt tcccggtggt gctggtggta
    69541 atggtgatat atcagcgtta cagcacgccg attgtccgtc gtgtgcgcgc ttatctggca
    69601 gatatcaacg acggctttaa cgaaattatc aatggcatga gcgttatcca gcagttccgt
    69661 cagcaggcgc gatttggcga acgtatgggt gaggccagtc gttcacacta tatggcgagg
    69721 atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc tgctgagtct gttttcatcg
    69781 ctcattcttt gtggcttgtt gatgctgttt ggcttctccg ccagcggcac cattgaagtg
    69841 ggcgtgttgt atgcgttcat cagttatttg gggcgactta acgaaccctt aatcgaactg
    69901 accacgcagc aggcgatgct gcaacaggct gttgttgctg gtgagcgcgt ttttgagctg
    69961 atggacggac cgcgccagca atatggcaat gatgatcgcc cgttgcagag cggcaccatc
    70021 gacgtcgata acgtgtcatt tgcttatcgc gatgacaatc tggtgctaaa gaacattaat
    70081 ctctctgtgc cttcgcgcaa ctttgtggcg ctggtcgggc ataccggcag tggcaaaagc
    70141 accctcgcca gtttattgat gggctattac ccgctaacgg aaggtgagat tcgccttgat
    70201 ggtcgtccat taagtacgct aagtcacagc gcgctgcgcc agggcgtggc aatggtgcag
    70261 caagatccgg tggtgctggc ggataccttc ctcgccaacg tgacgctggg acgtgatatc
    70321 tctgaagaac gcgtctggca ggcgctggaa atcgtgcagc tggcggagct ggcgcgtagc
    70381 atgagtgatg gtatttacac gccgcttggc gagcagggga ataatctctc agtcgggcaa
    70441 aagcaactgc tggcactggc gcgcgtgctg gtggagacgc cgcaaatcct gatccttgat
    70501 gaggcaaccg ccagcattga ctccgggact gaacaggcga ttcaacatgc tctggcggcg
    70561 gtgcgtgaac atactacgct tgtggtgatt gctcaccgct tatcgactat tgttgatgcc
    70621 aacaccattc tggtgcttca tcgtgggcaa gccgtggagc agggcactca ccagcaactg
    70681 ctggcggccc agggacgata ctggcagatg tatcaactgc aacttgcggg cgaagagctg
    70741 gcagccagcg tgcgtgaaga ggaatcattg agcgcctgaa tagcgcaata tttcatcgtt
    70801 ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt tttcattttc aatcttctta
    70861 acttcctgct ctctttctcg tttttcattt ttggcacacc gcttgcaata ccttcttcgt
    70921 gtagcagaac cattaccgaa ttctgaccgg aggggatcta tgaagctggt gaccgtgatt
    70981 atcaaaccat tcaaacttga agacgttcgt gaagcgttat cttccattgg tattcagggc
    71041 ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag gacatgccga gctgtaccgg
    71101 ggggcggaat acagcgtcaa tttcctgcca aaagtaaaaa ttgatgtggc gattgctgat
    71161 gaccaactcg atgaagtgat cgatatcgtc agtaaggcgg cttacaccgg aaaaattggc
    71221 gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc gtattcgtac cggcgaagcc
    71281 gacgaagcgg cgctgtaatc tctggcacac agcaacagga acgaaaaatg aagatagcga
    71341 cgataaaaac tgggcttgct tcactggcga tgctgccggg actggtaatg gctgcacctg
    71401 cggtggccga taaagccgac aatgcgttta tgatgatttg tactgcgctg gtgctgttta
    71461 tgactattcc ggggattgcc ctgttttacg gtgggttgat tcgcggcaaa aacgtgctgt
    71521 cgatgctgac gcaggtgacg gtgacgtttg cactggtctg tattctctgg gtggtttacg
    71581 gttactcgct ggcgtttggt gagggaaaca acttcttcgg caacattaac gggttgatgc
    71641 tgaaaaacat cgaactgacg gcggtgatgg gcagcattta tcagtatatc cacgtagcgt
    71701 ttcagggatc gtttgcctgc attaccgtag gcttgatagt tggagcactg gcggaacgaa
    71761 tccgcttctc agctgtgttg attttcgtgg tggtatggct gacgctctcc tacattccga
    71821 ttgcgcatat ggtctggggc ggcggtttgc tggcttctca cggtgcactg gatttcgcgg
    71881 gtggcacggt ggtgcacatt aacgccgcaa tcgccggtct ggtgggcgcg tatctgatag
    71941 gcaaacgcgt gggcttcggc aaagaggcgt ttaaaccgca caacctgccg atggtcttca
    72001 ccgggactgc cattctctat atcggttggt ttggctttaa cgccgggtca gcgggcacgg
    72061 cgaatgaaat cgcagcactg gcatttgtga atactgtggt cgcaacggcg gcggcaattc
    72121 ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc ttcactgctg ggagcgtgtt
    72181 caggcgcgat tgccggtctg gtcggcgtga cgccagcgtg tggctacatt ggtgttggcg
    72241 gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt gtggggcgtt accatgctca
    72301 aacgcttgtt gcgggtggat gatccctgcg atgtcttcgg tgtgcacggc gtttgtggca
    72361 ttgtcggctg tatcatgacc gggatttttg ctgccagctc gctgggcggc gtgggcttcg
    72421 ctgaaggtgt gacgatgggc catcagttgc tggtacagct ggaaagcatc gccattacga
    72481 tcgtctggtc cggtgtggtg gcatttattg gctacaaact ggcggatctg acggttggtc
    72541 tgcgtgtgcc ggaagaacaa gagcgagaag ggctggacgt caacagccac ggcgagaatg
    72601 cctataacgc gtaacaagca ctgcaaaaaa cagccggacg gttttcacct ccggctattt
    72661 ttttaattat gattacgcat taccccttcc tgaacggtcg aggcaaccag cacgccgtcc
    72721 tgagtataaa actcaccgcg cacaaagccg cgtgcgctgg acgccgaggt gctctccacg
    72781 ctatacagca gccattcatt caaattaaac gggcgatgga accacatgga atggtcgatg
    72841 gtcgctatct gaattcccgg ttcgagaaaa ccgatgccgt gcggctgtag agctaccggc
    72901 aggaagttaa gatcagaagc gtaaccgagc agatactgat gaacgcgcag atcatccggc
    72961 acgctaccat ttgcgcggat ccacacctga cgatgcggtt ctgcgacgtg acctttcaat
    73021 gggttatgaa attccaccgg acggacttcc agcggacgat cgcagatgaa tttatctttc
    73081 agcactggcg gcagcaggtg cgccagcgat tgggcgattt gtgtttccga aggcaggcca
    73141 tcaggcgctg gcgcggacgg cattgttttt tgatgttcga aacccgcttc tggtgcctgg
    73201 aaagaggccg tcatataaaa aatcggtttg ccgttttgaa tagcagcaac ccggcgggcg
    73261 ctgaagctgt taccgtcacg cagcgtttcg acatcataaa taatcggctt cttactatcg
    73321 cctgggcgaa gaaagtagct gtgaaacgaa tgtaccagcc gctcttcagg gacggtctct
    73381 tttgcagcat acaaggcctg acccacgacc tggccgccaa acacctggcg taaacctaaa
    73441 tcttcactct ggccgcgaaa gagtccttcc tcaatttttt ccagatttaa caatgtcagt
    73501 aaatttttta gcgcctgact catataactc tccagtaaca aagctgccgc agcaagccaa
    73561 agtgagttga gtataacgca aatttgccac tggtccgatg ggtgcaatgg tctgaattac
    73621 gggctaatta caggcagaaa tgcgtgatgt gtgccacact tgttgacgtt actattttgt
    73681 taaccactct tccggcgagg aaagttagcc cgctggtgca ttgataataa ggagaagtga
    73741 atgaaactcg tgcacatggc cagtggttta gcggttgcga ttgcgttggc ggcttgcgca
    73801 gataaaagcg cggatattca gacgccagca ccggctgcaa atacgtctat ttcagcaaca
    73861 caacctgcta tccagcaacc gaatgtctcc ggtaccgtct ggatccgtca gaaagtcgca
    73921 ctgccgcctg atgctgtgct gaccgtgaca ctttctgacg cgtcgttagc cgatgcgccg
    73981 tcaaaagtgc tggcgcagaa agcggtgcgt accgaaggta aacagtcacc attcagcttt
    74041 gttctgccat ttaacccggc agatgttcag ccgaacgcgc gtattctatt gagtgcggcg
    74101 attaccgtga atgacaaact ggtatttatt accgataccg ttcagccggt gatcaatcag
    74161 ggcggaacta aagccgacct gacattggtg ccggtgcagc aaaccgccgt gccggttcag
    74221 gccagcggtg gcgcaacggc taccgtacct tcgacttcac caactcaggt gaatccgtct
    74281 tcggcagttc cagctcctac gcaatattaa gcgcagttaa ccctctccgc ccggagaggg
    74341 ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata ccatcacacc
    74401 ttctgccagt aatgcctgtc gttgacgctg taaatccggt ccggttagcg aaattgtgcc
    74461 gtggcgatta accacccggt gccagggcaa ggtgctgcct tcggggagac gctttaacac
    74521 accgcctacc tggcgcgcgg cgcgcggcga tcccgccagt ttcgccacat caccgtaagt
    74581 ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg gaaatgaatc
    74641 ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa aaacgcccga
    74701 gtgaagccgc attgcgcaag aaaccagcat ctggcacgca atgggttgca attagccggg
    74761 gcagcagtga taatgcgcct gcgcgttggt tctcaacgct ctcaatgggg gctctgttgg
    74821 ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag ccaaggcaga
    74881 tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct ccccccgttt
    74941 cgtcaaaaaa tcccaacatg gctaaacttt aaccacaact gacgtcgcaa gaattgtctg
    75001 gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt tgttgttgcc
    75061 gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc gccattattt
    75121 aaagagttcg gtgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc ggatggtaaa
    75181 gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt ctttagctgg
    75241 attgaatacc cttcaaaaga ggtccgcgac gctgctaatc aaaagatgat gtcggaccca
    75301 cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat ctatggtgga
    75361 ttcgaatcaa tcatcgacga gtaggtagcg tgacgggctg tgcggcgtgc cgtcagccct
    75421 aaaattcaca aatgttgctc ggcccagcgt aagaaatcct ctttcggtaa tgccttgctg
    75481 tagagccagc cctgaccgta gtgcacgcca tgctggcgta accactcttc ttgtttactg
    75541 gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc gatgatatgc
    75601 ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt cagaatgtcg
    75661 acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc atcgagataa
    75721 atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt cggatcggca
    75781 aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata gtgattgatc
    75841 atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc aagattgatc
    75901 gaaatatgct gctgcggatg ctgacgcagc cagtcgccca tatcttcgaa gacgcttctt
    75961 atgatcagta gcgtcaacgg ctcagaaagg cccgtttgct gtgccagcgg aataaaacta
    76021 tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc agcaccgaca
    76081 attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc acgattttcg
    76141 atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg cagcacaaac
    76201 atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac ttgccgatgc
    76261 cagcctttct gcaacatttt cgttgaagac caggtgatga tcgaaatatt catttccggg
    76321 aagggcagga tatcgtaaat gattccatta ttttcgatat gctcaccggg tgttttttgt
    76381 agtctggtaa taattccctg agcaatttca tcgctgctgg ttatcacaac gttatgggca
    76441 ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc aataaaggaa
    76501 gcggggtcga tcatgacgac ataatgtgcc gttcccatgg caaccatgta acggataatg
    76561 cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat tttacctggc
    76621 tcggggaagg tatcgggcgg gctttcatgc tccagagacg aacactgggg aacgttgtta
    76681 tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg catttccatg
    76741 agatgggctt cgctacaggc agcgccttgc catctttcca gctcttgcag cgcatctttc
    76801 ccttgtgtcg ccaccttatt ggcccgaata gcgacgcggg aggaatacgt attcagctct
    76861 tcaataaatg atgtttctac ctgctgatgg gccagccaga tgcttaagcc gacaggcagc
    76921 aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt tctcaccagc
    76981 atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag gctggcagga
    77041 gataaggagg tagagccggg atttcatgaa gggatgggtg gaaacgaacg ttgtcatttg
    77101 accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg attcggcgcg
    77161 agcgagggta aaaatatcgt aaaaagatag ttcaccttta cggacgatca ttttttgcag
    77221 ttgttcttta tgaacatccg cgacacgcgg agagttcagg atggcttcaa ctagcgcggc
    77281 aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat ccagcacgtc
    77341 gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat taagcaggat
    77401 aaataataca accagtagcc agacagagcc tgaaagcgtt tcggactgca acatcactgc
    77461 cagggtggca aaaaaagcaa cgtacatacc gataaacaac cccggatgtt tacggataaa
    77521 actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtatttagag actcaattgt
    77581 ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct gcaactttcc
    77641 agggaacacc ctattttaac gtgggaatag tagagggaaa gtaacagatt taccacaatt
    77701 ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct ggattaccgc
    77761 ccacgacaac gttgtccggg acatcttttg tgacaactgc acctgaggct accacgacgt
    77821 tatcaccaat ggtcacacca gggttaatga ccgcacgtcc gccaatccag acgttattac
    77881 cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg tcgatgggat
    77941 gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcacca atgcgaatag
    78001 ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg agaaaaatgt
    78061 tataaccata gtcacagcga aacgttggct caatataagc ctctgtcacc tgaccgaata
    78121 aatcagcaag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga ttgtatcggt
    78181 gaataagccg acgagcgcgc aggcgatcgc gagataacgc ctcatctgcc gagtgataca
    78241 actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg ttgctgaaaa
    78301 tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttcccattac atctgtgtga
    78361 tgaagatcac atagggtatg ccttcatggc gaaagaatta taaaactctt aataaacagc
    78421 cggttatagc tccgaaagcg agttatttat tagcgaataa atttccatac tgaggaaggg
    78481 atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc ggctgagtaa
    78541 aataccgcca gctcattatc tgataattcg tatttatttt tctcgataac acgctccagc
    78601 gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt taaaggtttt
    78661 tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg caggattctc
    78721 tttcgtcgca ttgacaaaac aacggaatag tcgattacct gacttccgcc atttctgaag
    78781 atcctgcata ttaataccat aactactgaa caacataaag gtgtcatcca gatattcgtc
    78841 gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa gtgcgaaggt
    78901 cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg ggtcgttaac
    78961 ccagccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg tttcacagag
    79021 aaacttaagc tgcgcgatat catgtctttt gggtgagtat tcatccataa cgcgtcccct
    79081 tcttagcggt tgaactaacg gacacctttc gggatggaaa aaacttactg acctggactt
    79141 gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat cgcattatta
    79201 cgaaaaatta caattaggaa ggaagcggga caggactcac tttttcatac tagcactaac
    79261 ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat tatcgccaga
    79321 tgaaattggt aacactaata ccaggattgc tctgaatatg acgtaata
//
LOCUS       DAZKJR010000003        78113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKJR010000003 DAZKJR010000000
VERSION     DAZKJR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.003"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..1297)
                     /gene="icd"
                     /locus_tag="ACHOWS_000173"
     CDS             complement(47..1297)
                     /gene="icd"
                     /locus_tag="ACHOWS_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFQ0045825.1"
                     /translation="MESKVVVPAQGKKITLQNSKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1469..2122
                     /gene="rluE"
                     /locus_tag="ACHOWS_000174"
     CDS             1469..2122
                     /gene="rluE"
                     /locus_tag="ACHOWS_000174"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFQ0045826.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            2132..2593
                     /gene="nudJ"
                     /locus_tag="ACHOWS_000175"
     CDS             2132..2593
                     /gene="nudJ"
                     /locus_tag="ACHOWS_000175"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFQ0045827.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2647..3753
                     /gene="mnmA"
                     /locus_tag="ACHOWS_000176"
                     /gene_synonym="trmU"
     CDS             2647..3753
                     /gene="mnmA"
                     /locus_tag="ACHOWS_000176"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0045828.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYDGEVCLGGGIIEQRLPLPV"
     gene            3789..4430
                     /gene="hflD"
                     /locus_tag="ACHOWS_000177"
     CDS             3789..4430
                     /gene="hflD"
                     /locus_tag="ACHOWS_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFQ0045829.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4434..5804
                     /gene="purB"
                     /locus_tag="ACHOWS_000178"
     CDS             4434..5804
                     /gene="purB"
                     /locus_tag="ACHOWS_000178"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0045830.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5973..6644
                     /gene="phoP"
                     /locus_tag="ACHOWS_000179"
     CDS             5973..6644
                     /gene="phoP"
                     /locus_tag="ACHOWS_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFQ0045831.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6644..8104
                     /gene="phoQ"
                     /locus_tag="ACHOWS_000180"
     CDS             6644..8104
                     /gene="phoQ"
                     /locus_tag="ACHOWS_000180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFQ0045832.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8180..9301
                     /gene="roxA"
                     /locus_tag="ACHOWS_000181"
                     /gene_synonym="ycfD"
     CDS             8180..9301
                     /gene="roxA"
                     /locus_tag="ACHOWS_000181"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFQ0045833.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETSWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9447..10676)
                     /gene="pepT"
                     /locus_tag="ACHOWS_000182"
     CDS             complement(9447..10676)
                     /gene="pepT"
                     /locus_tag="ACHOWS_000182"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0045834.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10926..12062
                     /gene="potA"
                     /locus_tag="ACHOWS_000183"
     CDS             10926..12062
                     /gene="potA"
                     /locus_tag="ACHOWS_000183"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFQ0045835.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPADEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFTVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12046..12903
                     /gene="potB"
                     /locus_tag="ACHOWS_000184"
     CDS             12046..12903
                     /gene="potB"
                     /locus_tag="ACHOWS_000184"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFQ0045836.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12900..13694
                     /gene="potC"
                     /locus_tag="ACHOWS_000185"
     CDS             12900..13694
                     /gene="potC"
                     /locus_tag="ACHOWS_000185"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFQ0045837.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13691..14737
                     /gene="potD"
                     /locus_tag="ACHOWS_000186"
     CDS             13691..14737
                     /gene="potD"
                     /locus_tag="ACHOWS_000186"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFQ0045838.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14795..15256
                     /gene="ymfA"
                     /locus_tag="ACHOWS_000187"
     CDS             14795..15256
                     /gene="ymfA"
                     /locus_tag="ACHOWS_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFQ0045839.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPYSKKTRRKRKSRP"
     gene            15253..16038
                     /locus_tag="ACHOWS_000188"
     CDS             15253..16038
                     /locus_tag="ACHOWS_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0045840.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHAASSMSNVKGSVLLLFTTRE
                     PRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDLPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(16164..16985)
                     /gene="cobB"
                     /locus_tag="ACHOWS_000189"
     CDS             complement(16164..16985)
                     /gene="cobB"
                     /locus_tag="ACHOWS_000189"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFQ0045841.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17001..17912)
                     /gene="nagK"
                     /locus_tag="ACHOWS_000190"
     CDS             complement(17001..17912)
                     /gene="nagK"
                     /locus_tag="ACHOWS_000190"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFQ0045842.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17941..19185)
                     /gene="lolE"
                     /locus_tag="ACHOWS_000191"
     CDS             complement(17941..19185)
                     /gene="lolE"
                     /locus_tag="ACHOWS_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFQ0045843.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19185..19886)
                     /gene="lolD"
                     /locus_tag="ACHOWS_000192"
     CDS             complement(19185..19886)
                     /gene="lolD"
                     /locus_tag="ACHOWS_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFQ0045844.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19879..21078)
                     /gene="lolC"
                     /locus_tag="ACHOWS_000193"
     CDS             complement(19879..21078)
                     /gene="lolC"
                     /locus_tag="ACHOWS_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFQ0045845.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21340..22413
                     /gene="ycfT"
                     /locus_tag="ACHOWS_000194"
     CDS             21340..22413
                     /gene="ycfT"
                     /locus_tag="ACHOWS_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0045846.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMTSLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22541..25987
                     /gene="mfd"
                     /locus_tag="ACHOWS_000195"
     CDS             22541..25987
                     /gene="mfd"
                     /locus_tag="ACHOWS_000195"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0045847.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26131..27093
                     /gene="ldtC"
                     /locus_tag="ACHOWS_000196"
     CDS             26131..27093
                     /gene="ldtC"
                     /locus_tag="ACHOWS_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFQ0045848.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27176..27433)
                     /gene="bhsA"
                     /locus_tag="ACHOWS_000197"
                     /gene_synonym="ycfR"
     CDS             complement(27176..27433)
                     /gene="bhsA"
                     /locus_tag="ACHOWS_000197"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFQ0045849.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            27674..28306
                     /gene="comR"
                     /locus_tag="ACHOWS_000198"
     CDS             27674..28306
                     /gene="comR"
                     /locus_tag="ACHOWS_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFQ0045850.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(28368..28907)
                     /gene="ycfJ"
                     /locus_tag="ACHOWS_000199"
     CDS             complement(28368..28907)
                     /gene="ycfJ"
                     /locus_tag="ACHOWS_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFQ0045851.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(29134..30438)
                     /gene="ndh"
                     /locus_tag="ACHOWS_000200"
     CDS             complement(29134..30438)
                     /gene="ndh"
                     /locus_tag="ACHOWS_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFQ0045852.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(30838..31380)
                     /gene="ycfP"
                     /locus_tag="ACHOWS_000201"
     CDS             complement(30838..31380)
                     /gene="ycfP"
                     /locus_tag="ACHOWS_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFQ0045853.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(31403..32428)
                     /gene="nagZ"
                     /locus_tag="ACHOWS_000202"
     CDS             complement(31403..32428)
                     /gene="nagZ"
                     /locus_tag="ACHOWS_000202"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFQ0045854.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAREAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHSDPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(32439..33263)
                     /gene="thiK"
                     /locus_tag="ACHOWS_000203"
     CDS             complement(32439..33263)
                     /gene="thiK"
                     /locus_tag="ACHOWS_000203"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFQ0045855.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(33244..33885)
                     /gene="lpoB"
                     /locus_tag="ACHOWS_000204"
     CDS             complement(33244..33885)
                     /gene="lpoB"
                     /locus_tag="ACHOWS_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFQ0045856.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(33899..34276)
                     /gene="ycfL"
                     /locus_tag="ACHOWS_000205"
     CDS             complement(33899..34276)
                     /gene="ycfL"
                     /locus_tag="ACHOWS_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFQ0045857.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTLPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(34279..34638)
                     /gene="hinT"
                     /locus_tag="ACHOWS_000206"
     CDS             complement(34279..34638)
                     /gene="hinT"
                     /locus_tag="ACHOWS_000206"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFQ0045858.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            34972..37161
                     /gene="fhuE"
                     /locus_tag="ACHOWS_000207"
     CDS             34972..37161
                     /gene="fhuE"
                     /locus_tag="ACHOWS_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFQ0045859.1"
                     /translation="MLSTQFNRDNQYQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATTPVDDENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWAYIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(37221..38654)
                     /gene="ptsG"
                     /locus_tag="ACHOWS_000208"
     CDS             complement(37221..38654)
                     /gene="ptsG"
                     /locus_tag="ACHOWS_000208"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFQ0045860.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(38949..39746)
                     /gene="ycfH"
                     /locus_tag="ACHOWS_000209"
     CDS             complement(38949..39746)
                     /gene="ycfH"
                     /locus_tag="ACHOWS_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0045861.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPQDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(39757..40761)
                     /gene="holB"
                     /locus_tag="ACHOWS_000210"
     CDS             complement(39757..40761)
                     /gene="holB"
                     /locus_tag="ACHOWS_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0045862.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFHGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(40758..41399)
                     /gene="tmk"
                     /locus_tag="ACHOWS_000211"
     CDS             complement(40758..41399)
                     /gene="tmk"
                     /locus_tag="ACHOWS_000211"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0045863.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATLPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(41389..42411)
                     /gene="yceG"
                     /locus_tag="ACHOWS_000212"
     CDS             complement(41389..42411)
                     /gene="yceG"
                     /locus_tag="ACHOWS_000212"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFQ0045864.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKLRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(42414..43223)
                     /gene="pabC"
                     /locus_tag="ACHOWS_000213"
     CDS             complement(42414..43223)
                     /gene="pabC"
                     /locus_tag="ACHOWS_000213"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFQ0045865.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(43343..44584)
                     /gene="fabF"
                     /locus_tag="ACHOWS_000214"
     CDS             complement(43343..44584)
                     /gene="fabF"
                     /locus_tag="ACHOWS_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0045866.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(44672..44908)
                     /gene="acpP"
                     /locus_tag="ACHOWS_000215"
     CDS             complement(44672..44908)
                     /gene="acpP"
                     /locus_tag="ACHOWS_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0045867.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(45119..45853)
                     /gene="fabG"
                     /locus_tag="ACHOWS_000216"
     CDS             complement(45119..45853)
                     /gene="fabG"
                     /locus_tag="ACHOWS_000216"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFQ0045868.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(45866..46795)
                     /gene="fabD"
                     /locus_tag="ACHOWS_000217"
     CDS             complement(45866..46795)
                     /gene="fabD"
                     /locus_tag="ACHOWS_000217"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0045869.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(46811..47764)
                     /gene="fabH"
                     /locus_tag="ACHOWS_000218"
     CDS             complement(46811..47764)
                     /gene="fabH"
                     /locus_tag="ACHOWS_000218"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0045870.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(47832..48902)
                     /gene="plsX"
                     /locus_tag="ACHOWS_000219"
     CDS             complement(47832..48902)
                     /gene="plsX"
                     /locus_tag="ACHOWS_000219"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0045871.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(48983..49156)
                     /gene="rpmF"
                     /locus_tag="ACHOWS_000220"
     CDS             complement(48983..49156)
                     /gene="rpmF"
                     /locus_tag="ACHOWS_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0045872.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(49208..49729)
                     /gene="yceD"
                     /locus_tag="ACHOWS_000221"
     CDS             complement(49208..49729)
                     /gene="yceD"
                     /locus_tag="ACHOWS_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFQ0045873.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            49928..50512
                     /gene="yceF"
                     /locus_tag="ACHOWS_000222"
     CDS             49928..50512
                     /gene="yceF"
                     /locus_tag="ACHOWS_000222"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFQ0045874.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(50625..51584)
                     /gene="rluC"
                     /locus_tag="ACHOWS_000223"
     CDS             complement(50625..51584)
                     /gene="rluC"
                     /locus_tag="ACHOWS_000223"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFQ0045875.1"
                     /translation="MKTETPSVKIVAINADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            52157..55342
                     /gene="rne"
                     /locus_tag="ACHOWS_000224"
     CDS             52157..55342
                     /gene="rne"
                     /locus_tag="ACHOWS_000224"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFQ0045876.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVAETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            55476..55769
                     /locus_tag="ACHOWS_000225"
     CDS             55476..55769
                     /locus_tag="ACHOWS_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFQ0045877.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            55766..56260
                     /locus_tag="ACHOWS_000226"
     CDS             55766..56260
                     /locus_tag="ACHOWS_000226"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0045878.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIDR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(56355..57308)
                     /gene="flgL"
                     /locus_tag="ACHOWS_000227"
     CDS             complement(56355..57308)
                     /gene="flgL"
                     /locus_tag="ACHOWS_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFQ0045879.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(57320..58963)
                     /gene="flgK"
                     /locus_tag="ACHOWS_000228"
     CDS             complement(57320..58963)
                     /gene="flgK"
                     /locus_tag="ACHOWS_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFQ0045880.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDASGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDTGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(59029..59970)
                     /gene="flgJ"
                     /locus_tag="ACHOWS_000229"
     CDS             complement(59029..59970)
                     /gene="flgJ"
                     /locus_tag="ACHOWS_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFQ0045881.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(59970..61067)
                     /gene="flgI"
                     /locus_tag="ACHOWS_000230"
     CDS             complement(59970..61067)
                     /gene="flgI"
                     /locus_tag="ACHOWS_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFQ0045882.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(61079..61777)
                     /gene="flgH"
                     /locus_tag="ACHOWS_000231"
     CDS             complement(61079..61777)
                     /gene="flgH"
                     /locus_tag="ACHOWS_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFQ0045883.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(61830..62612)
                     /gene="flgG"
                     /locus_tag="ACHOWS_000232"
     CDS             complement(61830..62612)
                     /gene="flgG"
                     /locus_tag="ACHOWS_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFQ0045884.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(62783..63538)
                     /gene="flgF"
                     /locus_tag="ACHOWS_000233"
     CDS             complement(62783..63538)
                     /gene="flgF"
                     /locus_tag="ACHOWS_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFQ0045885.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(63558..64763)
                     /gene="flgE"
                     /locus_tag="ACHOWS_000234"
     CDS             complement(63558..64763)
                     /gene="flgE"
                     /locus_tag="ACHOWS_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFQ0045886.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMTLVFNANGVLTSDPTANISTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(64788..65483)
                     /gene="flgD"
                     /locus_tag="ACHOWS_000235"
     CDS             complement(64788..65483)
                     /gene="flgD"
                     /locus_tag="ACHOWS_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFQ0045887.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(65495..65899)
                     /gene="flgC"
                     /locus_tag="ACHOWS_000236"
     CDS             complement(65495..65899)
                     /gene="flgC"
                     /locus_tag="ACHOWS_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFQ0045888.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(65903..66319)
                     /gene="flgB"
                     /locus_tag="ACHOWS_000237"
     CDS             complement(65903..66319)
                     /gene="flgB"
                     /locus_tag="ACHOWS_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFQ0045889.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            66475..67134
                     /gene="flgA"
                     /locus_tag="ACHOWS_000238"
     CDS             66475..67134
                     /gene="flgA"
                     /locus_tag="ACHOWS_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFQ0045890.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            67210..67503
                     /gene="flgM"
                     /locus_tag="ACHOWS_000239"
     CDS             67210..67503
                     /gene="flgM"
                     /locus_tag="ACHOWS_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFQ0045891.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            67508..67924
                     /gene="flgN"
                     /locus_tag="ACHOWS_000240"
     CDS             67508..67924
                     /gene="flgN"
                     /locus_tag="ACHOWS_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFQ0045892.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(67964..69499)
                     /gene="murJ"
                     /locus_tag="ACHOWS_000241"
     CDS             complement(67964..69499)
                     /gene="murJ"
                     /locus_tag="ACHOWS_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFQ0045893.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(69609..70532)
                     /gene="yceM"
                     /locus_tag="ACHOWS_000242"
     CDS             complement(69609..70532)
                     /gene="yceM"
                     /locus_tag="ACHOWS_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0045894.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(70534..71181)
                     /gene="yceH"
                     /locus_tag="ACHOWS_000243"
     CDS             complement(70534..71181)
                     /gene="yceH"
                     /locus_tag="ACHOWS_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFQ0045895.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(71192..71776)
                     /gene="rimJ"
                     /locus_tag="ACHOWS_000244"
     CDS             complement(71192..71776)
                     /gene="rimJ"
                     /locus_tag="ACHOWS_000244"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0045896.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            72012..73220
                     /gene="mdtH"
                     /locus_tag="ACHOWS_000245"
     CDS             72012..73220
                     /gene="mdtH"
                     /locus_tag="ACHOWS_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFQ0045897.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            73284..73931
                     /gene="grxB"
                     /locus_tag="ACHOWS_000246"
     CDS             73284..73931
                     /gene="grxB"
                     /locus_tag="ACHOWS_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFQ0045898.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPIELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            74065..74625
                     /gene="yceB"
                     /locus_tag="ACHOWS_000247"
     CDS             74065..74625
                     /gene="yceB"
                     /locus_tag="ACHOWS_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0045899.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            74731..75777
                     /gene="pyrC"
                     /locus_tag="ACHOWS_000248"
     CDS             74731..75777
                     /gene="pyrC"
                     /locus_tag="ACHOWS_000248"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0045900.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVRKEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            75851..76096
                     /gene="dinI"
                     /locus_tag="ACHOWS_000249"
     CDS             75851..76096
                     /gene="dinI"
                     /locus_tag="ACHOWS_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFQ0045901.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            76386..76640
                     /gene="bssS"
                     /locus_tag="ACHOWS_000250"
     CDS             76386..76640
                     /gene="bssS"
                     /locus_tag="ACHOWS_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFQ0045902.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            76755..77873
                     /gene="solA"
                     /locus_tag="ACHOWS_000251"
     CDS             76755..77873
                     /gene="solA"
                     /locus_tag="ACHOWS_000251"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFQ0045903.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            77921..78034
                     /locus_tag="ACHOWS_000252"
     CDS             77921..78034
                     /locus_tag="ACHOWS_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFQ0045904.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVYYF"
BASE COUNT        18938 a        20720 c        19567 g        18888 t
ORIGIN      
        1 aacgggcgtg ttatacgccc gtttcaatat ttaacacatg tagagattac atgttcttga
       61 tgatcgcatc accaaactcg ctgcatttca gcagtttagc gccttccatc agacgttcga
      121 agtcataggt tacagtcttg gcgttgattg cgccttccat acctttaaca atcaggtctg
      181 cggcttcagt ccagcccata tggcgtaaca tcatctcagc ggagagaata atagagccag
      241 ggttaacttt gtcctgaccg gcatatttcg gcgcagtacc gtgggtggct tcaaacaggg
      301 cgcattcgtc accgatgttt gcgcctgggg cgataccgat accgccgacc tgtgcagcca
      361 gagcgtcaga aatgtagtca ccgttcaggt tcatacaggc gataacatca tattcagccg
      421 gacgcagcag gatctgttgc aggaatgcat cagcaatcac atctttaatg acgatctctt
      481 tgccggtatt cgggttttta actttcagcc acgggccgcc gtcgatcagt tcaccgccaa
      541 actcttcacg cgccagctgg tagccccaat ctttaaacgc gccttcggtg aacttcatga
      601 tgttgccttt gtgcaccagg gtcacagagt cacgatcgtt cgcaattgcg tattcgatcg
      661 ctgcacgcac cagacgtttg gtgccttctt cagaacacgg cttaataccg ataccgcaat
      721 gttccgggaa acgaattttc ttcacgccca tctcttcacg caggaattta atcactttct
      781 cggcgtcggc agagtctgct ttccattcga tacccgcata aatgtcttcc gagttttcac
      841 ggaagataac catatcggtc agttcagggt gtttaaccgg gcttggggtg ccctggtagt
      901 aacgtaccgg acgcaggcag atgtagagat ccagttcctg gcgcagggca acgttcagag
      961 agcgaatacc gccaccaacc ggagtggtca gcgggccttt aatggcaacg cgatattcac
     1021 gaatcagatc aagggtttca gcaggcagcc agacatcctg accataaacc tgtgtggatt
     1081 tttcaccggt gtaaatttcc atccaggaga ttttacgctc gcctttatag gctttctcga
     1141 ctgcagcgtc gaccactttc agcatggctg gggttacatc tacaccgatt ccatcacctt
     1201 caatgtaagg gataatcgga ttttcaggaa cgttgagttt gctgttttgc agggtgatct
     1261 tcttgccttg tgccggaaca actactttac tttccattca cctctccttc gagcgctact
     1321 ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta
     1381 gagcaatttt ttgttaatga tttgtaattg gcttgtcaat actaccctat tgtttgccgt
     1441 catgaaagat gcgcgttatt aggctataat gcggcaattc ataatctctg aaaataccat
     1501 gcaaaaaact tcttttagaa atcaccaggt taagcgattc agctcgcaac gttctaccag
     1561 gcgtaaacct gaaaaccagc ccacgcgtgt gatcctgttc aataaaccct acgatgttct
     1621 tccgcagttc accgatgaag ccggacgcaa aacattaaaa gaattcatcc cggttcaagg
     1681 cgtttatgca gcaggtcgcc ttgaccgcga tagcgaaggg ttactggtgc tgaccaataa
     1741 cggcgcgttg caggcgcgtt taacccagcc gggtaaacgt accgggaaaa tctattatgt
     1801 acaggtagaa ggtattccca cggaagacgc ccttgaagcg ttgcgcaatg gcgtaacctt
     1861 aaatgatggc cctaccctgc ccgcaggtgc agagatggtt gaggagccgg aatggttatg
     1921 gccccgtaat ccgccaattc gtgaacgcaa aagtattccc accagctggc tgaagatcac
     1981 cttatatgaa ggacgaaatc gccaggtgcg ccgcatgacc gcccatgttg gtttccccac
     2041 gctgcgactg attcgctatg cgatgggtga ttactctttg gataatcttg ccaatggcga
     2101 atggcgagaa gcgacagatt aaggacgcaa aatgtttaaa ccgcacgtta ccgttgcctg
     2161 cgtggtgcac gcagaaggca aatttttagt cgttgaagag acgattaatg gtaaagcgtt
     2221 atggaaccaa cctgccgggc atctggaagc ggatgagacg ctagtggaag ctgctgcgcg
     2281 tgagctgtgg gaagaaaccg gcatcagcgc gcagccgcaa cactttattc gtatgcatca
     2341 gtggattgcg ccagataaaa cgccgttttt gcgtttcctg tttgctattg agcttgagca
     2401 aatatgcccg acccagcctc atgacagcga tatcgactgc tgccgttggg tcagcgccga
     2461 agaaatttta caggcgtcaa atctgcgttc gccgctggtg gcggaaagta ttcgttgtta
     2521 tcaaagcggg caacgctatc cgctggagat gattggcgat tttaactggc cttttacaaa
     2581 gggtgtcatc taaaaggctg cgtgatagaa tacgccgcct tgaagttcaa tgtcgtgagt
     2641 gatccaatgt ctgaaaccgc aaaaaaagta atcgtcggca tgtccggcgg tgtcgattcc
     2701 tccgtttctg cctggctgtt gcaacaacag ggatatcagg tcgaaggcct gtttatgaag
     2761 aactgggaag aagacgacgg tgaggaatat tgcacagcgg cagcggatct ggctgatgcc
     2821 caggctgtct gcgacaagct cggcattgaa ctgcacaccg ttaactttgc tgccgagtac
     2881 tgggacaacg tcttcgaact gttccttgcc gaatataaag ccggtcgcac gccgaatccg
     2941 gatattctgt gcaacaaaga gatcaaattt aaagccttcc tcgaatttgc cgccgaagat
     3001 ttaggtgccg attatatcgc taccggtcat tacgtacgtc gggcagatgt cgatggcaag
     3061 agccgcctgc tgcgtggtct ggacagcaat aaagaccaga gctacttcct ttatacgctc
     3121 agccatgagc agattgcgca aagcctgttc ccggtcggcg aactggaaaa accgcaggtg
     3181 cgtaagattg ctgaagatct tggtctggtc accgcgaaga aaaaagactc taccggcatt
     3241 tgcttcattg gcgaacgtaa attccgcgag ttcctgggcc gttatctccc ggcgcaaccg
     3301 ggcaaaatca ttaccgtcga tggcgatgaa attggtgaac accaggggct gatgtatcac
     3361 accctcggtc agcgtaaagg tctgggtatt ggtggcacca aagaaggcac cgaagaaccg
     3421 tggtatgtgg tcgataaaga cgtcgaaaac aacattctga ttgttgcgca gggccatgaa
     3481 cacccgcgtc tgatgtctgt cggtctgatt gcccagcagt tgcactgggt cgatcgcgaa
     3541 ccattcaccg gcactatgcg ttgcacggta aaaacccgct atcgccagac cgacattccg
     3601 tgcaccgtca aggcgctgga cgatgatcgc attgaagtga ttttcgatga gccggttgcc
     3661 gccgtgacgc cgggccagtc tgccgtattc tatgacggtg aagtgtgcct cggtggcggt
     3721 attattgagc agcgtctgcc gctgccggtc tgattatttt ctttacttaa aacaaggaag
     3781 cagtgaacgt ggcaaagaat tactatgaca tcaccctcgc tctggccggt atttgtcagt
     3841 cggcacgcct ggtgcaacaa ctcgctcacc aggggcattg tgatgccgat gcgctacacg
     3901 tctcactcaa cagtattatt gatatgaacc ccagctcgac gctggcggtt tttggcggta
     3961 gcgaagccaa cctgcgcgtc gggctggaaa ccttgctcgg cgtgctcaat gccagcagtc
     4021 gccagggctt aaacgctgag ttaacccgct acacactcag cctgatggtg cttgagcgca
     4081 aactctcctc agcgaaaggt gcgctcgaca ctctgggcaa ccggatcaac ggcctgcaac
     4141 gccagctcga acacttcgat ttacagtccg aaacgctgat gagcgcgatg gctgctatct
     4201 atgttgatgt gattagcccg cttggcccgc gcattcaggt caccggttcc cctgctgtac
     4261 tgcaaagccc acaagtgcag gcgaaagttc gcgctaccct gctggcaggc attcgcgccg
     4321 ccgtgctctg gcaccaggtc ggcggcggac gtctgcaact gatgttttct cgtaatcgcc
     4381 tgaccactca ggcaaaacaa attcttgctc atttaacccc ggagttgtga tctatggaat
     4441 tatcctcact gaccgccgtt tcccctgtcg atggacgcta cggcgataaa gtcagcgcgc
     4501 tgcgcgggat tttcagcgaa tatggtttgc tgaaattccg tgtacaagtt gaagtacgtt
     4561 ggctgcaaaa actggccgcg cacgcagcga tcaaggaagt tcctgctttt gctgccgacg
     4621 caatcggtta ccttgatgca attgtcgcca gtttcagcga agaagatgca gcacgcatca
     4681 aaaccatcga gcgtaccact aaccacgacg ttaaagcggt tgagtatttc ctgaaagaaa
     4741 aagtggcgga gatcccggaa ctgcatgcgg tttctgaatt catccacttt gcctgtactt
     4801 cggaagatat caataacctc tcccacgcgt tgatgctgaa aaccgcgcgt gatgaagtga
     4861 tcctgccgta ctggcgtcaa ctgattgatg gcattaaaga tctcgccgtt cagtatcgcg
     4921 atatcccgct gctgtcccgt acccacggtc agccagccac gccgtcaacc atcggtaaag
     4981 agatggctaa cgtcgcctac cgtatggagc gccagtaccg ccagcttaat caggtggaga
     5041 tcctcggtaa aatcaacggc gcagtcggta actataacgc ccacatcgcc gcttacccgg
     5101 aagttgactg gcatcagttc agcgaagagt tcgtcacctc gctgggtatt cagtggaacc
     5161 cgtacactac ccagatcgaa ccgcacgact acattgccga gctgtttgat tgcgttgcgc
     5221 gcttcaacac cattctgatc gactttgacc gtgacgtctg gggttatatc gcccttaacc
     5281 acttcaaaca gaaaaccatt gctggtgaga ttggttcttc caccatgccg cataaagtta
     5341 acccgatcga cttcgaaaac tccgaaggga atctgggcct ttccaacgcg gtattgcagc
     5401 atctggcaag caaactgccg gtttcccgct ggcagcgtga cctgaccgac tccaccgtgc
     5461 tgcgtaacct cggcgtgggt atcggttatg cgctgattgc gtatcaatcc accctgaaag
     5521 gggtgagcaa actggaagtg aaccgtgacc atctgctgga tgaactggat cacaactggg
     5581 aagtgctggc tgagccaatc cagacagtta tgcgtcgcta tggcatcgaa aaaccgtacg
     5641 agaagctgaa agagctgact cgcggtaagc gcgttgacgc cgaaggcatg aagcagttta
     5701 tcgacggtct ggcgttgcca gaagaagaga aagcccgcct gaaagcgatg acgccggcta
     5761 actatattgg tcgcgccatc accatggttg atgagctgaa ataagcctca aatcagtgcc
     5821 ggatggcgat gctgtccggc ctgcttatta agattatcca ctttttgttt tttcacttta
     5881 cctccccttc ccgctggttt atttaatgtt tacccccata accacataat cgcgttacac
     5941 tattttaata attaagacag ggagaaataa aaatgcgcgt actggttgtt gaagacaatg
     6001 cgttgttacg tcaccatctt aaagttcaga ttcaggatgc tggtcatcag gtcgatgacg
     6061 cagaagatgc caaagaagcc gattattatc tcaatgaaca tttaccggat attgcgattg
     6121 tcgatctcgg tttgcctgac gaggacggtc tgtcactgat tcgccgctgg cgtagcaatg
     6181 atgtttcact gccgattctg gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag
     6241 tattaagtgc tggcgctgat gattacgtga ctaaaccgtt tcatattgaa gaggtgatgg
     6301 cgcgaatgca ggcattaatg cggcgtaata gcggtctggc ttcacaggtc atttcgctac
     6361 caccgtttca ggttgatctc tctcgccgtg aattatctat taatgacgaa gtgatcaaac
     6421 tgaccgcgtt cgaatacacc attatggaaa cgttgatacg caataatggc aaagtggtga
     6481 gcaaagattc gttaatgctc caactctatc cggatgcgga gctgcgggaa agccatacca
     6541 ttgatgtact gatgggacgt ctgcgcaaaa aaattcaggc acaatatccg caagaagtga
     6601 ttaccaccgt tcgcggccag ggctatctgt tcgaattgcg ctgatgaaaa aattactgcg
     6661 tctttttttc ccgctctcgc tgcgggtacg ttttctgttg gcaacggcag cggtagtact
     6721 ggtgctttcg cttgcctacg gaatggtcgc gctgatcggt tatagcgtca gtttcgataa
     6781 aaccacgttt cggctgttac gtggcgagag caatctgttc tatacccttg cgaagtggga
     6841 aaacaataag ttgcatgtcg agttacccga aaatatcgac aagcaaagcc ccacgatgac
     6901 actaatttat gatgagaacg ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat
     6961 gaagatgatc cagcctgact ggctgaaatc gaatggtttt catgaaattg aagcggatgt
     7021 taacgatacc agcctcttgc tgagtggaga tcattcgata cagcaacagt tgcaggaagt
     7081 gcgggaagat gatgacgacg cggagatgac ccactcggtg gcggtaaacg tctacccggc
     7141 aacatcgcgg atgccaaagt tgaccattgt ggtggtggat accattccgg tggagctaaa
     7201 aagttcctat atggtctgga gctggtttat ctatgtgctc tcagccaatc tgctgttagt
     7261 gatcccgctg ctgtgggtcg ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa
     7321 agaagtccgc gaactggaag aacataaccg cgaattgctc aatccagcca caacgcgaga
     7381 actgaccagt ctggtacgaa acctgaaccg attgttaaaa agtgaacgcg aacgttacga
     7441 caaatatcgt acgacgctca ccgacctgac ccatagtctg aaaacgccac tggcggtgct
     7501 gcaaagtacg ctgcgttctc tgcgtagtga aaagatgagc gtcagtgatg ctgaaccggt
     7561 aatgctggag caaatcagcc gcatttcaca gcaaattggc tactacctgc atcgtgccag
     7621 tatgcgcggc gggacattgc tcagccgcga gctgcatccg gtcgctccac tgctggacaa
     7681 tctcacctcg gcgctgaaca aagtgtatca acgcaaaggg gtcaatatct ctctcgatat
     7741 ttcgccagag atcagctttg tcggtgagca gaacgatttt gtcgaggtga tgggcaatgt
     7801 gctggataat gcctgtaaat attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga
     7861 cgagcatctc tatattgtgg tcgaggatga tggccccggt attccattaa gcaagcgaga
     7921 ggtcattttc gaccgtggtc aacgggttga tactttacgc cctgggcaag gtgtggggct
     7981 ggcggtagcc cgcgaaatca ccgagcaata tgagggtaaa atcgtcgccg gagagagcat
     8041 gctgggcggt gcgcggatgg aggtgatttt tggtcgccag cattctgcgc cgaaagatga
     8101 ataaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg ttgcagatac
     8161 cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg attttcttga
     8221 acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt ttattgaccc
     8281 gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagtcgaca gtcgactggt
     8341 cagtcatcag gatggcaaat ggcaggtcag ccacggtcca ttcgaaagct acgatcatct
     8401 cggtgaaact agctggtcat tgttagtgca agcagtgaac cactggcatg agccgaccgc
     8461 cgcgctgatg cgaccgttcc gtgaactgcc ggactggcgt attgatgacc tgatgatctc
     8521 tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg tgtttatcat
     8581 tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga aacagcactg
     8641 cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg aagagctgga
     8701 gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg cgctggaaaa
     8761 tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaactga ttagtggatt
     8821 tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc cggatgttcc
     8881 acctcgcgct catcctgcgg acgttctgcc gcaagagatg gataaactgc gtgagatgat
     8941 gctcgaattg atcaaccagc cggaacattt taagcaatgg tttggcgagt ttatatccca
     9001 gtcacgtcat gaactggata tcgcgccgcc agagccgcct tatcagccgg atgaaatcta
     9061 cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggg ggtctgcgcg tattgcgcat
     9121 tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc cggcactgga
     9181 tgcgctcgcc agcaacattg cgctgaccgc ggagaatttt ggcgatgcgc tggaagatcc
     9241 gtcattcctc gcgatgctcg cggcgctggt caatagcgga tactggttct tcgaagggta
     9301 agtttggatt taagccggat gcggcgctga ttgcgcctta tcgtgcctac atgcactgaa
     9361 cgcctgtagg cacgataagt atgtggacca gtgtcaatat aaaggtttca tcccggtaat
     9421 tggcagtcca catacgcatc gggcaatcac gacttccgtt gcgccgtcaa ctcggcaata
     9481 cggacgatca cctgcaccgc tttttccata ccttccagag tcacaaactc atgcttacca
     9541 tgatagttgt aaccgccagt gaacaggttc gggcacggta atcccataaa cgacaactgc
     9601 gcgccgtcgg taccgccgcg gatcggtttc agttccggtt caatatcgca gtcacgcatc
     9661 gcctgctggg cgatatcgag aatatgcgga tgctcaacca ctttctcgcg catattgtag
     9721 taactgtctt caatcaccag ttcaatgtag caatcaggat gtaacccttt gcccactttt
     9781 ttggcgatct ccatcatttt acgtttacgc gcttcaaact gtttacggtc gaaatcacgg
     9841 atgatgtagt gcatatcggc ccgttcaacg gtgcctttca tgctcgccag gtgatagaaa
     9901 ccttcatagc cttctgtcat ttccgggctt tcatccgccg gaacttccgc atgaatacgt
     9961 gccgccagcg acagcgcatt taccattact cctttcgccg tgcccgggtg aacattgtta
    10021 ccgacaattt tgatattgac cgacgcggcg ttgaagtttt caaactccag ttcgcctacg
    10081 ccaccaccat caacggtgta agcccagcgg gcatcgaagg cgtcaacatc aaaatgcttc
    10141 gcccctttgc ccacttcttc atccggggta aaggcgacgc gaatatcacc atgcggaatg
    10201 tttttttgtt gcaataccgc cagcgcggtc atgatttctg caatacctgc tttgtcatcg
    10261 gcacctaaca aggttttacc atcggtggta atcagcgtct gacccagtag ctgatgcagc
    10321 accgggaaca taaccggtga taaaacttca tcgccgatac ccagcgcaat atcgccaccg
    10381 cgatagtttt caacaatttg cggattcaca tttttgccgc tgcaatccgg tgaggtatcc
    10441 acatgagaaa taaagccaat cgccgggata tcgccaggga cgttagccgg taacgtcgcc
    10501 atcaaagtgc ccttctcact taaggtcaca ttgataagcc ccatctcttc gagctgctct
    10561 ttcagcagat gcaataactt ccattggcct tccgtgctgg gaacctgtct cacccctgcc
    10621 tttgattggg tatccagaga cacgtagttc aaaaatcgct caagtagttt atccatgtag
    10681 tcaccctcac tttttgtgac aacattatta agaagcaaga aaagacaaat attgcgtcag
    10741 gtcactttta gccctgcaag cgggaatatt tatcagcatt accttaatga atataaagct
    10801 aagccagtaa ttcacaacaa agattggcga ttcaagcggt ttgccgtaga attaccgccc
    10861 tcattaagag tcaccaaggt ggttaaccac aaaccccgca tcggtaagcc atccgttgcg
    10921 tttacatggg acagagtaaa aaattgaata aacaaccgag ttcgctttca ccgctggtgc
    10981 aattggcggg aattcgcaaa tgctttgatg gtaaagaggt cattccccag ctggatctga
    11041 ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg
    11101 ttcttcgcct gattgcaggt ctggaaactg ttgattccgg acgcatcatg ttggataacg
    11161 aggacatcac ccacgttccg gcggaaaacc gctacgtgaa cactgttttc caaagctacg
    11221 cacttttccc ccacatgacc gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa
    11281 cccccgctga tgaaattacg ccccgcgtga tggaagccct gcggatggtg cagctggaaa
    11341 ccttcgctca acgcaaaccg catcagctct ctggtggtca acagcaacgc gtcgccattg
    11401 cccgcgcggt ggttaacaag cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg
    11461 attacaaact gcgcaagcaa atgcagaacg agctgaaagc gttacagcgt aagcttggta
    11521 ttacattcgt gttcgtaact cacgaccagg aagaagcact taccatgtcg gacaggatcg
    11581 tggtgatgcg cgatggtcgc attgagcaag acggcacgcc gcgtgaaatc tacgaagagc
    11641 cgaaaaacct gtttgttgcc gggtttattg gcgaaatcaa tatgtttaac gccactgtca
    11701 tcgaacgtct cgacgagcag cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct
    11761 acgttaactt caccgttgaa ccggggcaaa aactgcatgt tctgctgcgc ccggaagact
    11821 tacgtgttga agagattaac gacgacaacc acgccgaagg gctgattggt tacgttcgcg
    11881 agcgtaacta caaaggcatg acgctggagt cggttgttga gctggaaaat ggcaagatgg
    11941 tgatggtcag cgaattcttc aatgaagacg atcctgactt tgaccactct ctcgaccaaa
    12001 aaatggccat taattgggta gaaagctggg aggtcgtact ggctgatgaa gaacacaagt
    12061 aagttccaga atgtagtgat tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg
    12121 cccaacctga tgatcattgg caccagcttt ttgacccgcg acgacgccag tttcgtcaaa
    12181 atggtcttta cgctggataa ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta
    12241 ttgcactcgc tgaatatggc gctgatcgcc accctcgcct gcctggtgct gggctatcct
    12301 tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc cgctgctgct gtttctgctg
    12361 attgttccgt tctggaccaa ctcattgatt cgtatttacg ggctgaaaat tttcctcagc
    12421 accaaaggct atctcaacga gtttttgctc tggctgggcg ttatcgatac accaattcgc
    12481 atcatgttca cgcctagtgc ggtgattatc ggtctggttt acattctgct gccgtttatg
    12541 gtgatgccgc tgtactccag tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc
    12601 gatctcggtg ccagcaagtt gcagaccttt atccgcatca ttatcccact aacgatgccg
    12661 ggaattattg ccggatgtct gctggtgatg ctgccagcga tgggcctgtt ctatgtgtcc
    12721 gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg tcatcaaggt acagttcctc
    12781 aatattcgtg actggccgtt tggtgcagcc accagcatta cgctgactat cgtaatgggc
    12841 ctgatgttgc tggtttactg gcgcgcttct cgtctgctga ataagaaggt ggaactcgaa
    12901 tgatcggtcg actgcttcgc ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc
    12961 caatcattat tttgattgtg aactccttta acagctcgcg ctttggcatc aactggcagg
    13021 gttttaccac caaatggtat agcctgctga tgaacaacga cagcctgtta caggcagcgc
    13081 agcattcact aacaatggcg gtgttttcgg cgacgtttgc tacgcttatc ggttcactga
    13141 cagcagttgc gctgtaccgt tatcgctttc gtggtaagcc gttcgttagc ggaatgctgt
    13201 ttgtggtgat gatgtcacca gatatcgtga tggcgatttc gctgctggtg ctgtttatgc
    13261 tgctgggtat tcagcttggc ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc
    13321 catttgtggt ggtgacagtg tattcgcgcc tgaaaggttt tgacgtgcga atgctggaag
    13381 cggcgaaaga tctcggtgcc agcgaattta ccattctgcg aaaaatcatt ctgcctctgg
    13441 caatgccagc ggtagcggcg ggctgggtgt taagctttac cctgtcgatg gacgacgtgg
    13501 tggtttcttc gtttgtcacc gggccgagtt atgaaattct gccgttaaaa atttattcga
    13561 tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc aaccatatta ctggtgctgt
    13621 cgctggtgat ggtaattgcc agccagctta ttgctcgtga taaaacgaaa ggtaacacag
    13681 gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg
    13741 catgagcgcc gctcacgccg atgacaacaa cacgctgtat ttctacaact ggaccgagta
    13801 cgtgccgcca ggactgcttg aacagttcac caaagaaacc ggtattaagg ttatctattc
    13861 gacttacgag tcgaacgaaa ccatgtacgc gaagctgaaa acgtacaaag acggtgccta
    13921 tgacctggtg gttccttcaa cctattacgt cgataaaatg cgtaaagaag ggatgatcca
    13981 gaagatcgac aagtcgaagt taagcaattt cagcaacctc gatccagaca tgctcaacaa
    14041 gccgtttgac ccgaataacg actattccat tccgtatatc tggggtgcaa cggcgattgg
    14101 cgttaacggt gatgcggtgg atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc
    14161 tgagtacaaa ggcagcctgc tgctgaccga cgatgcccgt gaagtgttcc agatggcgct
    14221 gcgtaagctg ggctactccg gtaacaccac cgatccgaaa gagattgaag ctgcatacaa
    14281 cgagctgaaa aaactgatgc caaacgtggc ggcgtttaac tccgataacc ccgctaaccc
    14341 gtatatggaa ggcgaagtta acctcggcat gatctggaac ggttctgctt tcgttgcacg
    14401 ccaggcgggt acgccaattg atgtggtgtg gccgaaagaa ggcggcattt tctggatgga
    14461 cagcctggcg atcccggcaa atgccaaaaa caaagaaggc gcactgaaat tgatcaactt
    14521 cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact atcggttatc caacgccaaa
    14581 ccttgcggcg cgtaagctgt taagtccgga agtggcgaac gataaaacgc tctacccgga
    14641 tgctgaaacc attaaaaatg gtgaatggca gaatgacgtt ggcgcagcca gcagcattta
    14701 tgaagagtat tatcagaagc tgaaagcagg acgttaattc agcaagcggg cggtggtatt
    14761 accgcccgtt ttttatttag caaggatgtg gctaatgagc caggacagta gagttttctt
    14821 tcgtatattc ttcggtatcg gtctggtact gatcctcatt tctgttgtta tcttttataa
    14881 ccagttcacc tatagcaaag acgcgattca tactgaaggc gtcattgttg ataccgtctg
    14941 gcactccagt cattctcatc gcacgggtaa aaatggttcg tggtatcccg tcgttgcctt
    15001 tcgtccgacg ccagattaca cgctaatttt caattcaagt attggcagcg atttttatga
    15061 agacagtgaa ggcgataaag ttaatgttta ttattctcca ggacatcctg aaaaggctga
    15121 gataaataac ccgtgggtta atttttttaa atgggggttt atcggcatta tgggcgttat
    15181 ttttatctcc gtcggtttat taatcagtat gccctactct aaaaagacac gcaggaaacg
    15241 gaagtcccgc ccatgaaaaa aatcataata ttgctcagtt tactgatact cctgcctctg
    15301 acggtaacca gtaagccatt gattccaata atgaagactc tttttacgga tgttaccggt
    15361 actgtaccgg atgccgaaga gatcgcgcgt aaagccgaac tttttcgtca acaaaccggg
    15421 attgcgccgt ttatcgtggt tgtacctggc gttaatgaag caagcttcag gcaaaatggc
    15481 aaagcgatgc tggcgcatgc ggcatcttca atgagtaatg taaaaggaag tgttctgtta
    15541 ctttttacta cccgcgagcc acggttaatt atgataacca acggccaggt tgaaagcagc
    15601 atggacgata aacatctcgg ccttctggta gaaaatcaca ctctggctta tttacatgca
    15661 gatctctggt atcagggaat caataatgca ttggctgttt tacaagcaca gatattaaaa
    15721 caaccgacgc cgccactgac atattatccg catcccggac aacagcagga gaatgatctt
    15781 cccggcagta ccacgacatt aggccttttt gcctgggcag ttgcattcat cgtctttgct
    15841 gcatttttta actacacaac gcgcctttat tatgcgttaa aatttgcagt ggctatggct
    15901 gttgccaata tgggctatca ggctttatgt ttgtatatcg ataacagctt tgcgattacc
    15961 agaatatcgc cgctctgggc agggctgatt ggtgtctgta cgtttattgc cgcgttacta
    16021 tggacgagta aacggtaaaa gaggcagtaa agttgccgga tgcggcgcga acgccttatc
    16081 cggcccacaa aacccgcaaa ttcaattaat tgcgcccctt cacaggcctg acaagcgtag
    16141 cgcatcaggc aatttttccc gctttataat cccttcagca acttttcaac aaactctggc
    16201 accacctggc ttgccggacc gtaatatttc tcggcaaatt cattaccaac ctgactcggt
    16261 tcaagattca gttccacggt gtgcgcgcca tgcagtttcg cttcgtgaac aaacccagcc
    16321 gccggataaa catgccccga agtaccaata gcaatgaaaa tatcggccat cgacaacgcc
    16381 atataaattt catccatgcc gagtggcatt tcgccaaacc acactacgtg cgggcgcaag
    16441 ggtgccggga actggcaaca atggcattta tcttctgggg taacatctcc ggtccagtcg
    16501 agaacctgac cactttgcga acagcgaact ttcagcagtt ccccatgcat atgaatgaca
    16561 ttggtattac ctgcgcgttc atgcaggttg tcgatattct gcgtcaccag caaaaagcga
    16621 tcgccgaggg cgtcttgcag tttcgccagc gcaagatgcg cggcgttagg ctgaatttct
    16681 ggctgctgca gctgtcgacg acgggcgtta taaaacgttt gcaccagttc aggatcgcga
    16741 tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag gccatctgcg
    16801 gcgcgaaagg tacgaatacc tgattccgca gaaatccctg cccctgtcag tacgagtact
    16861 cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg ctgacgcaaa
    16921 cgctcgcgca gacggcgttt atttttacga aaacgactta accgatgacc ccgacgcgac
    16981 agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc acgcattcct
    17041 cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg taagagatga
    17101 cgaggcagcc tgtccgccag ttgcgttgtg attgccggga aattcgataa gccaccacca
    17161 atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagacaaac cgctaataaa
    17221 tccagataac gctcaacgtg cgcccttgcc tgctcatcac cttgatcata aagcgcaata
    17281 atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc aaaaccgcga
    17341 ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg taacgggaaa
    17401 tccagcccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa ctcgccggta
    17461 atatagcttt taccggtaat gggtttaccg ttgaaaatca gcccgccgcc aacgccggtg
    17521 ccgagaatca accccatcac cagtggatat tgagtaaatt catcatccca tgcttcagaa
    17581 agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg
    17641 tcggcacgca gcggtttacc gctggcagca gggacattgg cggcatacag cgtaccatct
    17701 tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc aaaacgccgg
    17761 tcagcttcgg ctaccagctc gcacactgca tctaaaaatg cgtcatagct gtcacgcggt
    17821 gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt
    17881 tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa tgtactgcct
    17941 ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgtcgcg ccggatacca
    18001 acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt ccagccagtg
    18061 caattccgat ggcaggaagt caataaaata gatatcgctg gagaggaact gatgaccgat
    18121 caacttttca atccactcaa taatcggggt aagttgcagt gaaacaacta cgccgataat
    18181 cacaccacac aggctaccga acagccctgc cagcaatcca taccagacaa agatggcgcg
    18241 aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac tcttgtcttt
    18301 caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca ccagtaccat
    18361 cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc cgtaagtacc
    18421 aatccagctt ttaatataaa catagctgtt ggtcacttca cccgcatcgc gtaccagctt
    18481 attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg aacccatatc
    18541 aagatattgc tgggcatccg ccagcgggat catggcaaaa ctgtgatcga gttgaccact
    18601 caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca gtttatgctc
    18661 gggattcgag ttggggatca taatcgacac ccaatcgccc tgcttcactt tcagcgcatc
    18721 cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc gccaggcatc
    18781 cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgcgggt taacgccctt
    18841 cacctggatt gcgcgcagat tggcaccact ttctaccagt ccggtgaaat tgatatacgg
    18901 cgcagcggcg gcaatacctg gcactttttg cacgttatcc agggcttcct gccagttagt
    18961 ccacggttga tccaccgcct ctatttcgcc atgcggcacc accgccagaa tgcggttatt
    19021 cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg ccacgccaag
    19081 ggcaatgcca atggtagaaa tgacggagat cagcgacacc atgccgccgc gccgccgtcc
    19141 gcggctaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc cgcccccatc
    19201 aggctcagtt ccgccgtcag acgaccatca cgcatctcca gttggcggct catacgtttt
    19261 gccagttgca ggtcgtgagt aaccaccagg aaggcggtgc cctgcaagcg attcaattct
    19321 ccaagcaact ggaagatgct gtcggcgtta cgtgcatcga ggttaccagt aggttcatcc
    19381 gccagcacca gccgcgggtt attgaccagc gcacgggcaa tcgccacgcg ctggcgttcg
    19441 ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac cgcttttaac
    19501 atctccagcg cacggctgtt gatttcagcg ggctttttct tgccaatcag cagcggcata
    19561 gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata aataaagccc
    19621 agcttctggt tgcgcagttc tgctttcgcc gccgacgaca gtttgctcat cggctgaccg
    19681 ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg cagcaaggta
    19741 cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc cacgctgaaa
    19801 ctgacattgt gcagcacatc ggtttgcaca ctgccttcct gatagcgttt gcacaggttg
    19861 tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg tggcggcagc
    19921 gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca gcgcaataac
    19981 aatgacctgt aaaggttcga tagcgaccgg cagcgccgcg ccatcaagca ggacgccgat
    20041 tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg ctccgaggat
    20101 cgcaccgata atcccggcgc tggccccttg caccataaag accatcatga tttgtcgcgg
    20161 agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca ccattagccc
    20221 tagcgaggta ataatgttaa acgccgctac ggcaacaatc aggctcagca gcaagcccat
    20281 catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac gccagtcctg
    20341 ccatttgctg ccttcaggca gtttttgttg acttaatgag tcgactttca gcggctcatc
    20401 cagccacaaa cgccagccgg taatattgcc tgccggataa cgcatcagac gcgaggcatc
    20461 ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga aagtgccaat
    20521 cacattgaac aggcgctggc ttggaatacg ccccatcggc gtgaactggc tggcagatgg
    20581 caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcca gctgttcacc
    20641 gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga ccagatacgg
    20701 cgttagtgga tctttttgtg ccgggtcgat acccagcatc accccgaccg ccacgctgcg
    20761 cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa cgccgtccag
    20821 tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag aagagagaat
    20881 tgcctgtggc atcaggccaa ggatgttgtt ttgtagctcg cgctcaaagc cgttcatcac
    20941 tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag aaagccagga
    21001 gacgaaacga ccgaagcgat ccgctgcacg cccacgcatg taacgcaggc caataaatag
    21061 agcgacaggt tggtacatga aatccgtctg gttgctgtta gcaaagacac gagtatataa
    21121 gcgaaagcgg gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    21181 tgccagcaaa tcaatcggct aaagggcctc attttctcat cggccttgta gcctgatctt
    21241 tatctgaaaa ataaccaaat acagacagat acgcattaca ccacgcgact ggcccctcag
    21301 gatcaacagc atgttaaata aaaagagcgt gttgtcctga tgaaacaaaa agagctatgg
    21361 attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc ggtcattacc
    21421 ttttatccgc atctcaccac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg
    21481 atctatttca atctttacct tgcacccttt cgtatgccgg tttttttctt tatatccggc
    21541 tatttgattc gccgttatat cgacagcgta ccgtggggaa attgtctcga taaacggatc
    21601 tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc gttaagtgcg
    21661 ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc cgcgtatgcc
    21721 gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagttt gtggtatctg
    21781 tatgcgttaa ttgtttattt cgtggtatgt aaaattttta gccgtctggc gctgccacta
    21841 ttcgccttgt ttgtactgat gagtgtggcg gttaatttcg ttcccacgcc gtggtgggga
    21901 atgaacagtg tgatccgcaa tttgccttat tacagccttg gcgcatggtt tggcgcaaca
    21961 ttaatgacct acgttaaaga ggtgccgttg cgccgccatc tgctgatgac ttctttgctg
    22021 accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc gctggtatcg
    22081 attgtggtga tcatgaagct gttttatcaa tacgagcaac gtttcggtat gcgctccacc
    22141 agcctgctga atgtgattgg caccaacacc attgctatct acaccaccca tcgcattctg
    22201 gtagagatat tcagcttaac tctgcttgcg ccaatgaacg cggcacgctg gtcgccgcaa
    22261 gtcgaactga cactcctgat ggtttatccc tttgtcagtt tgttcatctg tactgttgcg
    22321 ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt ctccccgcct
    22381 tcactgcccg ctgccgtcag ttactcccgc taagccatcg ccccagccgg ggcgattcaa
    22441 tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag atatgccccc
    22501 atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac aatatcgtta
    22561 tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg gcgctgcctg
    22621 cgcgacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac ttattgcacc
    22681 agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagtttaccg atcagatggt
    22741 gatgaatctg gcggactggg aaacccttcc ctacgacagt ttttcgcctc atcaggacat
    22801 tatctcctcg cgcctttcca ccctttacca gctaccgacc atgcagcgtg gcgtactgat
    22861 tgttccggtg aatacgctta tgcagcgcgt ttgcccgcac agttttctcc acggtcatgc
    22921 gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc aactggacag
    22981 cgccggttat cgccatgttg accaggtaat ggagcacggc gaatacgcca cgcgcggcgc
    23041 gttgctggat ctcttcccga tgggcagtga gctgccttat cgtcttgatt tctttgatga
    23101 tgaaatcgac agcctgcgag tgtttgacgt cgatagccag cgcacgctgg aggaagtaga
    23161 agagatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa ttgaactgtt
    23221 tcgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata tttatcagca
    23281 agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccgctat tcttcagcga
    23341 accgctgccg ccactgttca gttatttccc tgccaatacg ctgttggtga atactggcga
    23401 tctggaaaac agtgccgaac gtttccaggc tgacacgctg gcgcgttttg aaaatcgcgg
    23461 cgtcgatccg atgcgcccac tgttgccacc acaatcgctc tggctgcggg tagacgagct
    23521 cttttcagag ctgaaaaact ggccgcgtgt gcagctaaaa actgaacatt taccgacaaa
    23581 agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc aggcacaaca
    23641 aaaagcaccg ctggatgcgc tgcgtaagtt cctcgagtct ttcgacggtc cggtggtgtt
    23701 ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcgc gaattaaaat
    23761 tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt atttgatgat
    23821 tggcgctgcc gaacatggtt ttgtcgatac ggtacgtaat ctggcgctga tttgcgaaag
    23881 cgatctgctc ggtgaacgcg ttgcccgtcg tcgtcaggat tctcgccgcg ccatcaaccc
    23941 cgatacactg atccgtaacc tcgcggagct gcatattggt cagccggtgg tccatctgga
    24001 gcacggtgtc gggcgctatg ccggaatgac cacgctggaa gcgggcggta ttactggcga
    24061 gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt cgtcactgca
    24121 tctgattagc cgttacgcgg gtggcgcgga agaaaacgcc ccgctgcata aacttggcgg
    24181 cgatgcctgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg tggcagcaga
    24241 attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta aacacgatcg
    24301 cgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg atcaggcaca
    24361 ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc gtctggtgtg
    24421 cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc tggcagtaga
    24481 taaccacaag caggtggcgg tgctggtgcc aaccaccctt ctcgcgcagc agcattacga
    24541 caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct cccgtttccg
    24601 cagcgccaaa gagcagacgc aaatccttgc ggaagtagcg gaagggaaaa tcgatattct
    24661 gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag gcctgctgat
    24721 tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag cgatgcgcgc
    24781 gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga atatggcaat
    24841 gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc tggcagttaa
    24901 aacctttgtc cgtgagtatg acagtctggt ggtccgggag gcgatcctgc gtgaaatttt
    24961 gcgcggagga caggtttatt atctctacaa tgatgtggaa aacatccaga aagccgccga
    25021 acggctggca gaactggtac ctgaagcgcg gattgccatc ggtcacgggc aaatgcgtga
    25081 gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg tgctggtttg
    25141 taccaccatt atcgaaaccg ggatcgacat tcctaccgcc aacaccatta tcattgaacg
    25201 cgcggatcac ttcggtctgg cacagttgca ccagttacgc ggtcgcgtcg ggcgttcgca
    25261 ccaccaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta ccgatgcgca
    25321 aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg cgctggcaac
    25381 gcacgatctg gagatccgcg gcgcaggtga actgcttggc gaagaacaaa gcggctcaat
    25441 ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgctgtcg atgcgctgaa
    25501 agccggacgc gagccgtcgc tggaagatct caccagtcag caaacagaag tcgagctgcg
    25561 gatgccgtcg ctattgcccg atgatttcat ccctgacgtg aacacgcgtc tgtcgttcta
    25621 caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag tcgagcttat
    25681 cgatcgcttc ggcctgctgc cggatccggc gcgtaccctg ctggatattg cccgtctgcg
    25741 ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag gcggcgtgat
    25801 cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc tgcaaaaaca
    25861 gccgcagcat tatcgcctcg atggtccgac gcgcctgaag tttattcagg atttgagtga
    25921 gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag agaacgcgat
    25981 cgcttaatgg taaatcgggg gcgtttctgc gcccccatct gcaacattta caaattcttt
    26041 gcacttccct gcactatccg acactgtcac catccataat tcaggcttat ctgtttatta
    26101 caataacctt atatttatta tggatttttg gtgatgatca aaacgcgttt ttctcgctgg
    26161 ctaacgtttt ttacgttcgc cgctgccgtg gcgctggcgc taccggcaaa agccaacacc
    26221 tggccgctgc cgccagcggg cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa
    26281 aatgacggtg gttctctgga agccatcgcc aaaaaatata acgtcggctt tctcgctctg
    26341 ttacaggcta accccggcgt tgatccttac gtaccgcgcg cgggcagcgt gttaacgatc
    26401 ccgttgcaaa ccctacttcc agatgcgccg cgcgaaggca ttgtgatcaa tattgcggaa
    26461 ctgcgtctct attactaccc gccgggtaaa aattcggtaa ccgtgtatcc catcggcatt
    26521 ggtcagttag gtggtgacac gctgacgccg acaatggtga ccaccgtttc agacaaacgt
    26581 gcaaacccaa cctggacgcc aacggcaaac atccgcgccc gttataaagc acagggaatt
    26641 gagttgcctg cggtagttcc ggctggaccg gataatccaa tgggccatca tgcgattcgc
    26701 ctggcggcct atggcggcgt ttatttgctc cacggtacga acgccgattt cggcattggc
    26761 atgcgggtaa gttctggctg tattcgtctg cgggatgacg atatcaaaac actctttagc
    26821 caggtcaccc caggcaccaa agtgaatatc atcaacactc cgataaaagt ctctgccgaa
    26881 ccaaacggtg cgcgtctggt tgaagtacat cagccgctgt cagagaagat tgatgacgat
    26941 ccgcagctgc tgccaattac gctgaatagc gcaatgcaat catttaaaga tgcagcacaa
    27001 actgacgctg aagtgatgca acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc
    27061 cgccgtcatc aagtgagccc acaaactctg taaagctcgg ataaaaaaat ccccgcgaca
    27121 cggcatgtcg cggggaaaaa aatcagcagt agcaggcatt aatgagggtt aatgcttatt
    27181 tataaattac tgctgttcca tggagggtat tcggaccggt tacagaagta atacggaaag
    27241 attttgcgcc catctcatcc gctttttgcg ccagctgctc ttccagcgat cccagatttg
    27301 tccccgcgtt agcactgata gtaccgactt tttgttggcc ttctggcgtt gactgaactt
    27361 cgacagccgc aaagctggca aatgacattg agcttaaaat cgccgcagcg atgagggttt
    27421 ttacgttttt cataatagtg gccttatgca gatgaatgac gtcgcaagca attacttaac
    27481 gatcgataac taaatgatag atgtgatctg gatcacatac aagatatttt ttataacaat
    27541 cattaattaa tttaaaaatc tttaaatttc agttaaatag aaagcaatta tttttatggt
    27601 ttgtggactg gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag
    27661 gtacaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gcggccgccc
    27721 aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg
    27781 ttatgaagcg acttctcttg cggacctcgt cgaagcgacc ggagccaaag cgcccacgct
    27841 atacgcggaa tttaccaaca aagaggggtt attccgcgcc gtactcgacc gctatatcga
    27901 tcgttttgcc gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc
    27961 gctggctgac tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg
    28021 ctgcttcatg atcaacaact gcaccaccct ctccccagat tcaggagata tcgccaatac
    28081 gctgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
    28141 agcgcgcggg gaaatcccga cccactgtga cgtgacgcat ctggcagaat tccttaattg
    28201 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca
    28261 gattgcccga acgactttgc gtttatggcc cgaactggtg aaataaggcg caaaaaaagc
    28321 ccctcagcct gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta
    28381 ttcaaaatca gttgcccgtt gctatctagc gggatctgcg tacccggatc gcggtccatg
    28441 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
    28501 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
    28561 agagagccct ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtagcg
    28621 acatctttac cgcgaccacc accaaactga tgccctatca cgccgccagc aacagcgccg
    28681 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg
    28741 ttacgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
    28801 tgagcgtatt gcgggccacg ttcaaacacg ttcagactgg ccactgccgc tacgcccaac
    28861 gcagctgcga caccaatccc gatacccgcc aacattgatt tattcacggg aatacctcct
    28921 tctttgcagt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg
    28981 aaaagcgcgg gaatggctgg aaatacttag ggattcggag gagtctgaag gaatgcctga
    29041 tgcgacactt aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggataa
    29101 ggcgtttatg ccccatccgc cagtacaagc cgattaatgc aacttcaaac gtggacggat
    29161 aacgcggtta atactcccca ccagcatcat taatccagtt ttaaagtaac catgcagcgc
    29221 aatctgatgc attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat
    29281 cattgagccg cgtgtcaggt tacccatcag gctaccgacg gtggaaaagt tcgacagcga
    29341 taccagcgaa ccgtgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc
    29401 cagaatgttg ttcattgcgc aggttgccat ctgatgtgcg gcctgagcgc gcggcggaac
    29461 aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtata tgtctggatc
    29521 gcgagtggtt tgcagcgtcg gttccaccac cagctggttg atacggttgg tttcaagacc
    29581 accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc
    29641 ctcaatatat tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt
    29701 ttgcgtcagc acgcgaacgc caagtttcgt taactcacta tgggccgcag cagagatacg
    29761 tggcggcaat gcaggcaaaa tacgttcgcc cgcttctacc agcgttacgt tcagggcttc
    29821 gttggtcaga cctttataac cgtaactgtg cagttgcttg accgcgttgt gcaattcagc
    29881 ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgtttactt tgccattcgc
    29941 gcccaggttg gcggagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc
    30001 ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt
    30061 ggaggtgcta cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag
    30121 caattcacct ttttcgtcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat
    30181 atcaattacg gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct
    30241 caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca
    30301 caggtggctg tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt
    30361 atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatttt
    30421 tttcaatggc gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt
    30481 tattaacata gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcactattga
    30541 gaataacatg aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctg
    30601 aaaagatagg cgactttacc gggcgagatc aaaaaacaga gtttattgtt tattatttga
    30661 gcgaatttgt gagagtgata tagagtgagg ggtgtatgga gtgcgttcgg taggcctgat
    30721 aagaggcgca tcaggcattg atgctcgact gtcggatgcg gcgtggacgc cttatccgac
    30781 ttacagaatt cggagcgcat caggtaccgg caaggttacc tgatgcgacg agggcattta
    30841 cccgagggtt ttgaacgcct taatgcgctg caaatgcggg gagatattct tgaatttgtg
    30901 cgtctgctct tcgtcccaga caatctcgta ataatgatgc agctcttcag atgtccgctg
    30961 gctgttaaga gcttcatcat tacgcgacaa aatcaccaaa caacgatcgc gattcttttc
    31021 gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc gatcaatctt
    31081 gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc ggatatcgca
    31141 aagaaaacca atccgttccg cccagtatcc gcccagacca acgccgcaaa tcagcggacg
    31201 ctcgtcgacg ttcagttgca acattttgtc cacttctttg agcagatgct gcatatcatg
    31261 tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa actgcaattg
    31321 taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat agataatcat
    31381 cgcttcctca cataagccag ggttagtgac ctgctttctc ttcctgccag cgttcatgta
    31441 actgattcag acgggcgctg atcgctttcc agcgagccga gtccatcagt tcctgtcgcg
    31501 aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt gacagattat
    31561 ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg caacccgcgt
    31621 ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggctcct tccatcgata
    31681 aatcgtcaga gaaaatcacg ccatcaaaac ccagttcctg acgcaaaacg gttttcagcc
    31741 aatagggaga accgctcgcc ggacgcggat cgacatcact gtagatcaca tgcgcaggca
    31801 taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata tctttagcgc
    31861 gaatctccgc ttgcggacgt gggtcgcacg gtgtttcttt gtgtgagtct gccgttactg
    31921 caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc ataccatcaa
    31981 taaaccggct ggcaattgcc agggcttttt cgggatcgga atgataagaa cgctcgccaa
    32041 tcgccgcgct gatatgcccg acgtccagca ctggcgcaaa gctgatatca atatccatag
    32101 caatcatttc gctggccatc aaccaacctg cctctcgcgc cagttttccg ccttcttcca
    32161 ttcccaacag cgcagcaaat gattgtgccg ctggcaagcg ggtaaaacct tcacgaaaac
    32221 gctgcacgcg tccaccttcc tgatcaaccg ccaccaccag atgattgcgc gaagctgcgc
    32281 ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc gtaaagagaa
    32341 tcagccctcc caccagcgga tgcgccagta tttcacgctc ttccgcgtcc agttcgtaac
    32401 cttcgacatc caacattact ggacccacac tgctctcctt attgttttat taatagctgc
    32461 cgccaggtgt catcggccag cctgataaat tgttgatcgc cggtttgtcg ccagcggtac
    32521 tcaaaccacc ctgctttgag catcagcagc cagggaaacc atcgcctgac ctgacgccat
    32581 aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg ccggtgctgg
    32641 tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc tccggcatac
    32701 tcccagtcga tgagttttaa ccctgacgcg ctatgcacta aatttccggc gtggacatcc
    32761 atatgcaatg gacttaagcg taaaggccgt ggttcccgcg ctttgcgcag acgttttaac
    32821 cttcgcagcc aacccactgt ccgccgcgcc ggatcgcttt gctgccagta cagttccagt
    32881 aacggcaaca gcgttattcg ccagccaaaa cgcggttgtt gatgtagata atacagcaag
    32941 cctgccagtt cgttggtatc cggcaaatac gtttttacct cgccgggcag atagtcgacc
    33001 accatccagt cacggagata taaatgcggc ttcggtgcaa tgcatgcggg tagttgtgat
    33061 aaagcccgat actggcgcaa gaacgcgaac cgcggcgcat caggatcgtg cggctgacgc
    33121 acaacaaaac gctggccctg atgttcaatg agaaaactcc cgccactaag cccactatta
    33181 aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc gcgcgtgatg
    33241 ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg tctgcaccag
    33301 catcagctgc atttgaaggg tcggagcgtt aacattgcca gaggcgctgg agtacagcac
    33361 gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgggtgc ctaaactgtc
    33421 ctgcggcgac aaacctaact gttgctttgc catcgacagc tgctgggcgg aaaccagggt
    33481 aaatttaccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg cggcattcag
    33541 cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg cagtcacgcc
    33601 gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt cataatggcg
    33661 aatatgcggc gcaggcggtg cagtttgatc ttcgtgctca attgggcctg gctgctgcgg
    33721 tatcgtcggc accgatggca ctgtggggac aggctgttgc ggctcggctg gttgttccgg
    33781 cgctggtttc acttcttcta ccggcgcagg ttcacgttgc cccacacacc cggcgagaaa
    33841 catcgccagc gcggtaatca aggcgtagcg actcattttg gtcatcaaga ttcacccctt
    33901 acaaatatag ataaagtctg actttgtgcg cccccagaaa attggcgctg ccgtacagcg
    33961 ttaccgccga atgtgcggga agggtaacgc tgcgtggcct ttccagagga tgcatctcca
    34021 gccctctggc gtcataccag taaaaacgat aatgaacggt aacgggttct tgcctttcgt
    34081 tataaagcgt tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg
    34141 cactgatgcc cgcagccaat aaagatgact ccatcaccag cgattgctca tcattcaccg
    34201 gaatttccgg atgtgaacga cagcccacca gcactaacaa cgccagagac accagcccaa
    34261 agcatccttt tctcattgtt acagaccttt atgcgccagc attggtccca gtggacggcc
    34321 acccaacaaa tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt tggtgttcat
    34381 gatcaggcga tagccatctt cggcaatacc ttcttgctca gcaatttttg ccgctacggt
    34441 gatcatgcgt cccagcgcct gctcatgctc agctgacacg tcgttcacag tcggaatgag
    34501 gatattcgga atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt
    34561 taccagatca tcctggtaga cgatatcgga ggggatctca cgacgaataa ttttgctgaa
    34621 tatagtttct tctgccacga cattttcctt tttcaaatag ccatgcgttg cgccatgcta
    34681 cgccgcgcca cactgcgagt atagagtatg gtcgagttaa acctgctctt tcaaccttaa
    34741 ctcacgattt cttaagcaaa aaatcgtttt gctgccgtat atatcgccat tattcccatt
    34801 tctgcctgcg aatgcgctga aaagacattc tctgtttggt ttacacgtca aatatgaatg
    34861 cgtatatttc tcatttgcat ttacatgcaa aattattcgc acactaaata taaccgctca
    34921 tcaccctcct ccggcatgag cctgacgaca acataaacca agagatttca gatgctttca
    34981 acacaattta acagggataa tcaatatcaa accacctcca aaccgtcact gcttgccgcc
    35041 tgcatagcac tggcactctt accttccgcc gcttttgctg caccagccac tgaagaaacg
    35101 gtgattgttg agggttcagc cactactcca gttgatgacg aaaatgatta cagcgtaacg
    35161 tctacctctg cgggtaccaa aatgcagatg actcaacgtg atattcctca gtcggtcact
    35221 attgttagtc agcagcggat ggaagatcag cagttacaaa cgctgggcga agtgatggaa
    35281 aacacgctgg ggatcagcaa aagccaagcg gattccgatc gtgctcttta ttattcccgc
    35341 ggattccaga tcgataacta tatggttgat ggtatcccca cctattttga atcgcgctgg
    35401 aatctgggcg acgcactttc tgatatggca ctgtttgaac gcgtagaagt agtgcgtggc
    35461 gcgacaggac tcatgaccgg gacgggtaat ccatctgcgg caattaatat ggttcgaaaa
    35521 cacgcgacca gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag ctggaacaaa
    35581 gaacggtatg tggcggattt acaaagccca ctcaccgaag atggtaaaat ccgcgcacga
    35641 attgtcggcg gctaccagaa taacgactca tggctggacc gctacaacag tgaaaagacc
    35701 ttcttctccg gcattgtcga tgctgattta ggcgatctta cgacgctttc agccggttac
    35761 gaatatcagc gcattgatgt taatagccca acctggggcg gtttaccgcg ctggaatact
    35821 gatggcagca gcaacagtta cgatcgcgca cgcagtaccg cacctgactg ggcgtataac
    35881 gataaagaga tcaacaaggt ctttatgacc ctcaagcagc ggtttgctga tacctggcaa
    35941 gcgacactga atgccaccca ctccgaagtc gaatttgaca gtaaaatgat gtatgtcgat
    36001 gcctatgtaa acaaagcgga tggtatgctg gttgggccat acagtaatta tggacctggc
    36061 tttgattatg tcggcggtac cggttggaac agcggcaaac gtaaagttga tgcgctggat
    36121 ttgtttgctg acggtagtta tgaattgttt ggtcgtcagc acaatctaat gtttggtggc
    36181 agttacagca aacaaaacaa tcgttacttc agttcatggg cctacatctt cccggatgaa
    36241 attggcagtt tctacaactt taatggcaat ttcccacaaa ccgactggtc accacagagc
    36301 ctggcgcagg acgataccac acatatgaaa tcgttatatg ctgccactcg tgtcaccctt
    36361 gccgatccgc tgcatctgat cctcggcgca cgttatacca actggcgggt tgatacgctg
    36421 acttacagca tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt tgacatcaat
    36481 gacaactggt cgacctacgc cagctatacc tctattttcc agccgcaaaa tgatcgtgac
    36541 agttcaggca aatatctggc tccaatcacc ggtaacaact acgagctggg tctgaaatcg
    36601 gactggatga atagccgtct gaccaccacg ttagccatct tccgtattga gcaggataat
    36661 gtcgctcagt ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg
    36721 gtggatggga cagtcagtaa aggtgtggaa tttgaactca acggcgcaat taccgacaac
    36781 tggcagctga cgtttggcgc aacgcgctat attgcagagg ataacgaagg aaacgccgtt
    36841 aatcctaatc tgccacgcac cacggttaaa atgttcacca gctaccggtt gcctgtcatg
    36901 ccagagttga cggtcggcgg tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg
    36961 acaccgtatg gcaccttccg cgccgagcaa ggtagctacg cgctggtgga tctcttcacc
    37021 cgctaccagg tgacgaaaaa cttctcgtta caggggaatg tcaataacct gttcgacaaa
    37081 acctacgata ccaacgtgga aggttctatt gtctacggcg caccgcgtaa tttcagcatt
    37141 accggcacgt atcaattctg aataacacct gtaaaaaagg cagccatctg gctgccttag
    37201 tctccccaac gtcttacgga ttagtggtta cggatgtact catccatctc ggttttcagg
    37261 ttatcggatt tagtaccgaa aatcgcctga acaccagaac cagcaaccac tacgcccgct
    37321 gcgcccagtt tcttcaggcc agcctgatcc actttagaca catcagcaac gctgacgcgc
    37381 agacgggtaa tacatgcgtc gaggttagta atgttttctt taccaccaaa tgcagcaacc
    37441 agagccggtg ccatttcgct ggtacctgtc gcttttgcgt cttcagtcgc gtcttcacga
    37501 cccggcgttt tcagatccag tgctttaatc agcacgcgga agatggtgta gtaaacaatg
    37561 gcataaccga taccgacgat cgggaacagc cacagtttgc tgctgttacc agacagaacg
    37621 atgaagtcga tcagaccgtg cgagaacgac gtaccgtcac gcatccccag aagaatacag
    37681 attgggaatg ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac
    37741 atgaaggaga actcgatcgg ctcggtgata ccggtcagga acgaggtcag cgccgcggag
    37801 atcataatac cgcccacttt cgcgcggttt tctggtttag cagagtgcca gatagcaatt
    37861 gcggcagctg gcagaccgta cattttgaac aggaagccac cagacagttt acccgcagtc
    37921 gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg
    37981 tattcaccaa tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc
    38041 aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc
    38101 cactgagaga aggtctggat tgcagaacca atcggcggcc aaatgaagga cagcacaacg
    38161 ccagtaaaga tggcagccag gcctgaaatg atcggcacaa agcgtttacc ggcaaagaag
    38221 ccaagatact caggcagctt aatacggtag aaacggttaa acatgtacgc tgcgatcgca
    38281 ccggagataa tccccccgag tacgccagta tccgccaggt gtttagaggc gatttcttca
    38341 gcaggtaaat gcagtaccag tggcgcaacc acggccatgg ttttaaccat gatgccatag
    38401 gcaacaactg cggccagcgc ggatacgcca tcgttattgg taaagccgag ggcgacaccg
    38461 atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc cataacatgc
    38521 gatacaacgg cgggcagcca gctgaaattc gcggaaccga cgcccagcag aatacctgcg
    38581 ataggcagta cggataccgg cagcatcagc gatttaccga ccttttgcag gttagcaaat
    38641 gcattcttaa acataattga gagtgctcct gagtatgggt gcttttttta cgttttcacg
    38701 cgtggcaagg ggggagagcc tcgccgtgta cagggcatct aagcgccctt tatttattac
    38761 acagagtaaa ataattcagt gccgatttgt ttgacggcta tcacgtttca acttcagatc
    38821 attggagaag ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac gtggttattc
    38881 accgcccaat tttaggcggt gaactttact cgttttagcc attaattacg agctttaaaa
    38941 aaactcattc aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag
    39001 ttatcggtgg ttacctgcgc cagttcttca acggcaacac ctttcaacac agccatgtat
    39061 tctgcaacgt cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg aaccggtgca
    39121 aggtaaggtg agtcagtttc taccagtaac cgatcctggg ggacataacg cgcggcatcg
    39181 cgcagttgct ccgcattacg gaaggtcaca atgccggaaa aggagatgta aaatccgaga
    39241 tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa
    39301 tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg cgcgggcgtc acgggtatgg
    39361 acgataaccg gcttgttcag ttcacgacca atctggatat gatggatgaa ggattcttgc
    39421 tgccgtactt tagtttccgg cgtgtaataa taatccaacc cggtttcacc cagcgcaaca
    39481 acgccctctt ctgccgccag acggcgtaaa tcttctacat cgtagggatc attctggttg
    39541 agtggatgca cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag atcccgcatg
    39601 tgtaaataac ccggtaatgt tgtggcgact gccagacaaa atttcacatc gcgtgcggcg
    39661 gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt cataatccag accatcgaga
    39721 tggcagtgtg agtcgactaa aaacatgatg tctctcttaa agatgaggaa ccggtagcac
    39781 aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg
    39841 gttaatacct gtgacagaca ttaactgttc acgaatgtgg caaacatccc ccagtatagc
    39901 ctgcaggcgc gagggagaaa gatggtttgc cagttcgacg accaggcccg gcacatcaac
    39961 attggtcacc tgcgcagcgc catgatggcg ttttagcgca tccatcagca acgttgccag
    40021 ccagtgtaaa cgcgccggag cttgttcatg attaagggcc gctagcagcg aataccagtc
    40081 gcccgatggc acgctatatg ccaaagcctg acacaatgtt tcacgagcct gccagttatc
    40141 tccgtgaaac aacgccagtg ccgcgccagg cgaaccggca cttaagcgca atgcggcaag
    40201 taatgcatcc tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt actgttccgg
    40261 cggcggcgca aggtaatgta accgacaacg actacgtaat gttgccagta aacgttctgg
    40321 ctcgcgggta gcaaggaaaa accacgtttc tgctggtggc tcttcaagcg ttttcagcaa
    40381 tgcgttagcc gcggcgtcgg tcagtaaggc agcatcggtt acccagacaa ctttcgcacc
    40441 acctaagcgt gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat caatgcccag
    40501 cgtatttttc cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat
    40561 caactgacat ccacgacagt gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa
    40621 ataacggctc agggcgtaga ttaaagcatc atcgcccatg cccggtaacg cctgaatgag
    40681 cagcgcatgg tgacctcttc cggcctgata gctggctacc agtttttcga aatcaggtcg
    40741 taaccatgga taccatctca tgcgtccaac tccttcaccc agttggtcac ggtagtgcgg
    40801 attgcatcca tcacagcctc cagcggcagg gtggcatcaa tggtatgaat gcttttatct
    40861 tgtgctgcca gttccagata gcgggcgcgg gtgcgattaa aaaaatcgaa agactcttgc
    40921 tcaatacgat ccagctcgcc gcgtgcacgc gcgcgtttta agccaacttc cggggtaaca
    40981 tcgagataga gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg cagtgttgcc
    41041 agcatatgtt ggtcaatacc acgtccgccg ccctgatacg cctgagtgga gagatcgtgg
    41101 cgatcgccaa tcacccaagt gccgttagcc agcgcaggtt tgatgaccgt ttctaccagt
    41161 tgaacgcgcg cggcataaaa catcagaact tcggctttat cggtaatgac ttcatcgcct
    41221 accgatttga tatccagcac caggcttctt aacttttcgg caagttgcgt accgccaggt
    41281 tcccgagtga aaaccatgtc gcggataccc agttgctcaa gcgtctcaac caccacgtta
    41341 cgcgcggtag ttttgcctgc gccttccagc ccctcaatga cgatatactt actgcgcatt
    41401 tttttcctta agcactttca gataatcctg cacagacttg ttatgactgg caagattggt
    41461 attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg tttttgccgg
    41521 atgcgcagca gccttcagcg aatccgcccc cggcgtcgct atcgcacctg gcggcaaacc
    41581 ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc
    41641 attataacgc tctcccatcc cgtaaatcac ggtcgggtcg gtctgcaggc gcataccaat
    41701 gcgtaaccgg ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa cggcggtttc
    41761 tttttcgata attgatgcca tcgtcaccaa ctggttttta tctttataag gcagaccgtc
    41821 cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg ctcgcttgag
    41881 taacgcgaca tcggtggtat tggcggtata catccaggtg tctggccaga accaaccttc
    41941 aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact tatcgtcgct
    42001 cagcgtgtgc ttgatatagg gggcctcacg caattgcttg aggtaatcgc tcagacgcat
    42061 cccttctacc agtcgcagag ggaactgtgc ttctttaccg ctttccagca atttcagcat
    42121 ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc
    42181 cggttcgata cgcagcagcc attggaaaac ccgtgggcga ttgatgatct tatcggcata
    42241 aagctgttca ccgagcgcca gacgtccggt ccctggcttc agggtaaata tcgtctcttc
    42301 tttgataagt aatttgctgt cggcaagatg gcgaagcttc cagacgccca caccagcggc
    42361 gatacccagt accaccagca ataacaagat aatcaataac acttttttca tgactaattc
    42421 gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa ggagacatcg
    42481 ccacaggcac gtacgggcat cactggcatt aacgcattac aaataaccat ctcatctgcc
    42541 tgcaacgcct cctccagcga ggcttgcact tcgacaagct gataagagga ttgtgccagc
    42601 aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag tcgcggcgta
    42661 taaactacgt tgcccttccg ccagaacaaa ttagccgcac agcattccgt aacccaccct
    42721 tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga gcggatcaat
    42781 acttgctcaa gccgattaag atgtttaata cctgcaagat gaggattgcg ccccagccgc
    42841 accgggctta gcgccaacgt catcccctcg ttacgcaaac ggtcgtaatg tgcgggataa
    42901 accgtaacgg agagaatccg cgttgccggt ccgctgttca atgtgctgta tcctcgcccg
    42961 ccactaccgc gactgatcac cactttcagc acaccatttt gctgttctgc tgccagcgtt
    43021 ttcatctcta gttcaagctg aggccagaag tcacaggaaa tcatcaaccg ctgacaagca
    43081 tcctgtagtc gctggatatg cgccgataac aaactgactt taccgtcgat aactctggcg
    43141 gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag cgattcctgc
    43201 ttatgaccgt taattaagaa cataccggct ccttatcaga gaattgttag tgtggcagca
    43261 tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt
    43321 tataagggtg ggaaatgaca acttagatct ttttaaagat caaagaacca ttagtgccac
    43381 cgaagccgaa ggagttacac agcgtgtatt ccattccgct gacctgacgt gcttcgtgcg
    43441 gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt ggcggaacag
    43501 cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc gcacctaaca
    43561 ggtgaccggt catagatttc gtggagctta ccaacacacg gcttgcagct tcaccgaaga
    43621 tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta ccgtgagcgt
    43681 taacgtagcc aatctgactc gcttcaatgc ctgcatcacg cagagcattt gccattgcca
    43741 gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg ctgctcatac
    43801 caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt tcgtactctt
    43861 caagtaccag cataccggca ccatcgccca gtacaaaacc atcacgctct ttatcccacg
    43921 ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac
    43981 caccaacgcc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc acgtcagcat
    44041 cgccatacgc gataatacgc gcagcatggc caatgttgtg cacgccggaa gtacaggcgg
    44101 tcgcgataga gatgcttggg ccacgcaggc catacatgat agtcagatga cctgccacca
    44161 tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca ccgttcatca
    44221 gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca attgcggcac
    44281 caatgcgggt tgcgttctct tccgttattt caaggccaga atcctgcatg gcctgaacgc
    44341 cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg cgcgagataa
    44401 tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag gcgctagtat
    44461 cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc caggtagact
    44521 ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca cgacgcttag
    44581 acacgtttgt cctccaggga gggaaaaaat gattctagtg ggacaaaaag ataaaactca
    44641 ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg gtggccgttg atgtaatcaa
    44701 tggcagcctg aacggtggtg attttctcag cttcttcgtc cggaatctca gtatcaaact
    44761 cttcttccag agccattacc agctcaacgg tgtcaagaga atccgcgccc aggtcttcaa
    44821 cgaaagaagc attgttggta acttcttcct gcttaacgcc cagctgttcg ccgataattt
    44881 tcttaacgcg ttcttcgata gtgctcatac tcttaaattt cctatcaaaa ctcgctttcg
    44941 cgatggtttt cgtagtgtat aaaatgttga aaaatttgca actaaatccc ggcaggtctt
    45001 accacgattt tacgttattt tgaggcctta ccccccaata acgcaaatca ttttgcacta
    45061 attattactc ataaccacgc agactgcgcg caacatgagc attttgtgca aatcgcggtc
    45121 agaccatgta catcccgccg ttcacatgca aagtttcacc cgtgatgtaa gctgcttcgt
    45181 cggatgccag gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg cgacccgcag
    45241 gaacctgcgc caggataccc gcacgctggt catcgctcag cgcacgtgtc atgtccgttt
    45301 caataaagcc cggagcaaca acgtttacag taataccgcg tgacgcaact tcgcgcgcca
    45361 gtgatttact gaagccgatc aagcccgctt tcgccgcagc gtagttggcc tgaccgccat
    45421 ttcccatggt accaaccaca gaaccgatag tgataatacg accatgacgc tttttcatca
    45481 tagcgcgcat taccgctttt gacagacgga aaacagatga aaggttggtt tcgataatat
    45541 cgttccactc ttcatctttc attcgcatta acaggttatc acgagtgata ccggcattat
    45601 tgaccaggat atccacttca ccaaattctg cgcgaatttt ttccagaaca gattcgatag
    45661 atgccgggtc ggtcacattc aacatcagac ctttgccgtt ggcacctaaa taatcagtga
    45721 tcgcctgagc gccattttca ctggtcgcag tgccgataac tttcgcgcca cgggctgcga
    45781 gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt gcgatttttc
    45841 cttcaaaatt catgattttc ctcttttaaa gctcgagcgc cgctgccatc gctgaaggtt
    45901 cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca gtaagcactt
    45961 tgcccgggcc gacttcatag agatgttcta cgccttgcgc tgccatgtat tcaacagact
    46021 tcgtccactg aaccgggtta tacaactgac gtaccagtgc gtcacggatg gcatcaccat
    46081 tggtttcgca tttcacatca acgttattca caacaggaac tgttggtgcg ttaaaggtga
    46141 ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt catcagcgca cagtgagacg
    46201 gtacgctcac tggtaacggc agagcgcgtt ttgcgcccgc agctttacag gcagcgccag
    46261 cacgctcaac tgcttcttta tgaccggcaa taaccacctg tcccggagag ttaaagttta
    46321 ccggagaaac gacctgacct tctgcagctt cttcacacgc tttcgcaata gacgcatcat
    46381 ccagaccgat gattgccgcc atagcacccg ttccttccgg tacggcttct tgcatgaact
    46441 tgccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc aatcacacca gcgcaaacca
    46501 gcgcggagta ttcccccagg ctgtgaccag ccatcattgc cggtgcttta ccgccctgct
    46561 gctgccatac gcgatacagc gcaacagatg cagtcaacag cgctggctgg gtttgccagg
    46621 ttttattcag ttcttcagcc ggcccctgct gggtcagcgc ccacaggtcg tagcccagcg
    46681 ccgcagaagc ttcagcaaac gtttcttcga caattggata gctcgccgcc atatcagcca
    46741 gcattccaac ggtttgagaa ccctgtccag ggaacacaaa tgcaaattgc gtcatgtttt
    46801 aatccttatc ctagaaacga accagcgcgg agccccaggt gaatccaccg ccaaaggctt
    46861 caagcagaac caactgcccc ggcttaatgc gcccgtcgcg tacagcttca tccagagcgc
    46921 acgggacaga ggccgcagag gtattaccgt ggcgatccag cgtcaccacg acattgtcca
    46981 tcgacatgcc gagttttttc gctgttgcac tgataatacg caggttagcc tgatgcggaa
    47041 ccagccagtc cagttgagaa cggtcaagat tattcgccgc cagcgtctca tctacgatgt
    47101 gcgccagttc tgttaccgca accttgaaga cttcgttgcc cgccatcgtc agatgaattg
    47161 aattctctgg attcacacgg tcagcattag gcagcgtcag caactcacca tagctaccgt
    47221 cggcatgcag atgggtggag atgattcccg gctcttcaga ggcagccagc accgcagcgc
    47281 ccgcgccatc gccaaaaata ataatagtcc cacgatcggt tggatcgcag gtgcgcgcca
    47341 gtacatcgga accgacgacc agagcatact tcaccgcccc agatttcacg tattgatcgg
    47401 ctacgcttaa tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat gccgggcaac
    47461 ctttaatgcc cagcatgctt tgaatctgac aagctgcgct cgggaaagcg tgcgtagcag
    47521 aagtcgttgc cacaacgatc aggccaatct ggtctttctc aatgcccgcc atctcaattg
    47581 cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc gtttggcgcg gcaatgtggc
    47641 gttcgcggat accggtacga gtgacaatcc actcgtcaga ggtgtccacc attttttcca
    47701 aatcggcgtt tgtccgcact tgttcgggca gatagctgcc agtaccaata atcttcgtat
    47761 acatgtacgc tcagtcactt ttcggttata taccgtcact tgcaaactgc gagttcgctg
    47821 gcagcgtcct gctaccgcag agttccgctt ttgccaccgt ccagcagctc aaaaccagct
    47881 gggtatacag attccaggcg agcggcaatt cgctgaggaa cttgtcgctg caccgcctgc
    47941 actgcctgtt caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg actttttatc
    48001 accgtgccgc gcaatcctaa cagacaggcg ccgttatact ggtcggggtt gaggtgactg
    48061 aatcgcctcg tcaggctctt ttgtagccaa cgctttaata acagtagcca ccacgaccgt
    48121 tttttccctt caccctgaga tttcagcaga gaaaggaaca tcctgacaac accttccatc
    48181 gtctttaatg tgacatttcc tgtaaagccg tcacaaacca gcacatctgt cttgccagtt
    48241 aacaactcat tggcttcaag atagccgata taattgatag aagggattgt tttaagcacc
    48301 gctgaggcat cccgaatact gtcgagaccc tttacttctt cttcaccaat attgagcaac
    48361 gccacgcgag gattaggaat ttccaccacc tcttcagcca gaactgagcc cataatggca
    48421 aattgcacca acattgtact gtcacaatcg acgttggccc ctaaatcgag gaccaccgtt
    48481 ttgccctttt gctgatgcgg taataccgtc accagcgccg gacgctcaat cccctccagg
    48541 ggcttgagta ataattttgc cagccccatt agcgccccgg tattaccggc actgacacag
    48601 gcttgcgctc gaccttcttt caccagctcc agtgccacgc gcattgaact cccacgactg
    48661 gcgcggatag cttgcgaagg ccgggcatca ctggcgataa ctgactgcgc aggaataatc
    48721 tgcagacgcg aacgttgttc aaagtcagct ttagcaagta atggcgtgat ggcgtcggga
    48781 ttgccgacta aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa tgctgcaggc
    48841 actgtcacgg aagggccaaa atcccctccc atgacatcta acgccagggt tagacgtgtc
    48901 aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa tcctcactaa
    48961 gcttcatcac gcagacgcgt gattacttag cgatgacctt gcggccgcgg tagtaaccgt
    49021 cggcagtgat gtggtgacgc aggtgttttt caccagaagt tttgtctaca gacaggctgg
    49081 tgactgcggt cagcgcgtca tgggaacgac gcatgccacg tttggaacgg gttggtttat
    49141 tctgttgtac ggccatggac cttactcctc aattacggtt tatccccgat ccaacgggga
    49201 gcaccaatta cttacgcttt aagctggcta atacggcaaa tgggtttggc ttttgcgctt
    49261 cttcaggcag ttcaccaaag accatgtccg cttcggacac ttcacagtgt tcagaatcat
    49321 gcaccggaac taccggcaag gcgaggatga tttcatcttc aaccattgca agcagatcga
    49381 tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc cggcagtgct tcagcctgtt
    49441 cgtctgaacg cacaggacta aaacaatacg ttgtgtagac ctgatgagta aacggcttcc
    49501 cgcaacgctg acactcgagc gttaccgtca ccttcgcatc gccgtttaac actgcgagac
    49561 gttggttatc gatagcgaac gacatggagc attccacatc actgtccaca ctgactacgg
    49621 attcggcgac gcgctcaacc tgatcaggag tatagatacc ctggtaatca aggcgttttt
    49681 gagccgtacg aaccggatcg agagtcaggg gtaattttac cttttgcata gggcgcgcat
    49741 attaactttg taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc cttttgccaa
    49801 taattcgcac acattgcggg ttactgcttt attttcgttc agtgctgata acgaacatca
    49861 acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc aatacgctat atctgatgga
    49921 aaaaaatatg cctaaactta ttttagcctc cacctcgccc tggcgtcgcg ctcttctgga
    49981 aaaactgcaa atctcttttg aatgtgcagc acccgaggtc gacgaaactc cacgcagcga
    50041 cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa aaagcgcaat ctctggcgtc
    50101 acgttatccg gatcatttaa ttattggttc agaccaggta tgtgttcttg atggcgaaat
    50161 caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa ttacgcaaag ccagcggcaa
    50221 tatcgtcacc ttctatactg gactggcgtt gtttaattcg gcgaatgggc atctacaaac
    50281 agaagtggag ccttttgacg tccatttccg tcacctgagc gaggcggaga ttgataatta
    50341 cgtgcgtaaa gagcatcccc tgcactgcgc gggtagcttt aagagtgaag gatttggcat
    50401 tacgctgttt gagcgtttag aggggcgaga tcctaacacg ctggttggtt taccgctaat
    50461 cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg ctgatgggat agttttctgc
    50521 gccggatgcg gttacgtcgc atccggcaat cggtgctggc ggcctgatgc ggcgtaaacg
    50581 ccttatcaag gccaaccgcc cattatcagg tttatatgct tgtattagcg cgcgttacgc
    50641 agcttttgca aacaacgctt caaaccttca tccatcggcg cttcgatacg catcacctca
    50701 ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg atttaatcct
    50761 gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca
    50821 atcggatgac ccgcatactg tgtatgcaca cggatctgat gagtacgccc tgttaccggg
    50881 ctacaacgca ccagggtggc aaaagcatag cgttcttcca ctttaaagcg tgtttccgac
    50941 ggtttgcctt cctgactcac acgcacgata cgttcgccgc tttgcagaat atttttcagc
    51001 aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag cgccaggtaa
    51061 tctttttgca tccctttttc acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt
    51121 ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac
    51181 cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc cgaagcttaa accactgccg
    51241 ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatggtcatc ttcatataag
    51301 atgacgtccg ccagcgccgc caccttttgc agatgtggcg aaaccgcctc ttcttcccgt
    51361 tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg cttcgagttt atattcaggc
    51421 ttaatacgtt ttttgttcac ccgcacttcg cctttacgca aaatacggta aatcatactt
    51481 tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca
    51541 gcgttgatag caacaatttt tacggatgga gtctctgttt tcatggtggg cgattctaat
    51601 tagccaacag gattcgcgcc actcattttt ctatgcttat atttagtttt gcaccttatt
    51661 acttcactac gtgatcactt tattgatggt tattaaacca atcaccagca agaagtgaaa
    51721 aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca aggcttgcag
    51781 tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt tacggaataa
    51841 cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg atctttcagg
    51901 cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc tggagagtaa
    51961 tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg ggcaaccgac
    52021 actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact agacacgagg
    52081 ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa
    52141 taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg aagagttgcg
    52201 cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc cagggcacga
    52261 gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga gtctggaagc
    52321 tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag aaattgcccg
    52381 cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag atgtgttgcg
    52441 tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca aaggcgcggc
    52501 attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccga acaacccgcg
    52561 cgcgggtggc atttctcgtc gtatcgaagg cgacgatcgt accgaattaa aagaagcact
    52621 ggcaagcctt gaactgccgg aaggcatggg acttatcgtg cgcaccgctg gcgtcggcaa
    52681 atctgctgag gcgctgcaat gggatttaag cttccgtctg aaacactggg aagccatcaa
    52741 aaaagccgct gaaagccgcc ctgctccatt cctgattcat caggagagca acgtcatcgt
    52801 tcgcgcattc cgcgattact tacgtcagga tatcggcgaa atccttatcg ataacccgaa
    52861 agtgctcgaa ctggcacgcc agcatatcgc tgcattaggt cgcccggatt tcagcagcaa
    52921 aatcaaactg tacaccggcg agatcccgct gttcagccac taccagattg agtcacagat
    52981 tgagtccgcc ttccagcgtg aagttcgtct gccatctggt ggttccattg ttatcgacag
    53041 caccgaagcg ttaaccgcca tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat
    53101 cgaagaaacc gcgtttaaca ccaacctcga agctgccgat gagattgctc gtcagctgcg
    53161 cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc cagtacgcca
    53221 ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccggg cgcgtattca
    53281 aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgtcagcgtc tgagcccatc
    53341 actgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta ccgtgcgtga
    53401 caacgaatcg ttgtcgctct ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa
    53461 cactcaggaa gttcacgcca ttgttcctgt gccaatcgcg tcttacctgc tgaatgaaaa
    53521 acgttctgca gtgaatgcga ttgaaactcg tcaggacggc gtgcgctgtg tgattgtgcc
    53581 aaacgatcag atggaaaccc cgcactacca tgtgctgcgc gtgcgtaaag gggaagaaac
    53641 tccaacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga
    53701 agaagagttc gctgaacgta agcgtcctga acaacctgcg ctggcaacct ttgccatgcc
    53761 ggatgtgccg ccagctccaa ccccagctga acctgccgcg cctgttgtag ccccagcacc
    53821 taaagctgca acggcaacac cagcatctcc tgcacaacca gggttgttga gccgcttctt
    53881 cggcgcactg aaagcgctgt tcagcggtgg tgaagaaacc aaaccggccg agcaaccagc
    53941 accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa
    54001 ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtgctgaag gcaacgataa
    54061 tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag cagactgccg agacgcgtga
    54121 gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc agcaagcgcc
    54181 gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt caggcgcaac aagaagcgaa
    54241 ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaggaac gtgtacgtcc
    54301 ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt
    54361 agccgaagaa gcggtagtcg caccggtggt tgaagaaact gtcgctgccg aaccaattgt
    54421 tcaggaagcg ccagctccac gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca
    54481 aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg gtggcatgcc
    54541 gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta
    54601 tcgtgacgag cgttatccaa cccaatcgcc aatgccgttg accgtagcgt gcgcgtctcc
    54661 ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc aagatgtaca
    54721 ggtcgaagag cagcgcgaac aggaagaagt acaagtgcag ccgatggtga ctgaggtccc
    54781 tgtcgccgcc gctgtcgaac cggttgttag cgcgccagtt gttgaagaag tggctgaagt
    54841 tgtagaagcc cccgttcagg ttgccgaacc gcaaccggaa gtggctgaaa cgacgcatcc
    54901 tgaagtaatt gccgccgcgg taactgaaca gccgcaggtg attaccgagt ctgatgttgc
    54961 cgtagcccag gaagttgcag aacacgcaga accggtggtt gaaccgcagg aagagacggc
    55021 tgatattgaa gaagttgccg aaactgctga ggttgtggtt gctgaacctg aagttgttgc
    55081 tcaacctgcc gcgccagtcg tcgctgaagt cgcagcagaa gttgaaacgg tagccgcggt
    55141 tgaaccagag atcaccgttg agcataacca cgctaccgcg ccaatgacgc gcgctccggc
    55201 accggaatat gttccggagg caccgcgtca cagtgactgg cagcgcccta cttttgcctt
    55261 cgaaggcaaa ggtgccgcag gtggtcatac ggcaacgcat catgcctctg ccgctcctgc
    55321 gcgtccgcaa cctgttgagt aataattagc tcaagtaatc aagccctggt aactgccagg
    55381 gcttttttat ttcaggggta gcatctttgc tgtaatgaca ttagcgttac atgtactgta
    55441 taattatcat ccacgcaaag ccgaaggata tatttatgcc agcagccaat aacattccag
    55501 ccaggcgcga atctttaaac ttgcgcataa aacccgcaga acgcgatctt attgaccggg
    55561 ccgctaaagc ccgaggaaaa aatcgcaccg aatttgtcct tgaggccgcc agagccgcag
    55621 ctgaagaggc gctcattgag cagagaattt tcaggatcga ttctgatgat tatcagctat
    55681 ttttgaatcg tctggatcag ccccccgcgc ccaatgctgc attacgcaaa accatgcaaa
    55741 cacccgcgcc gtgggagcaa aaaaaatgat tcaggccccc gagccactca tggcaaggca
    55801 tcaattcacc tctttttgct cgggagtaga aacgatggat aactggctaa agcagagagc
    55861 cctaaaaaat cagcttgccg gcgcatcacg tacatttgtt agctgtgata cgtatagcaa
    55921 cgttttggca tattattcgc ttgcttccag cgccgttgaa acgtatgtcg cgacaggacg
    55981 atttcgccga aatatgccag aaccgatccc tgttgtagta cttggtcgtc tggcaataga
    56041 taaatcatta cagggtcagg gcatcgatcg tgcgatggtc agagatgccg gtctacgagt
    56101 actacaggcg gctgaagtca ttggtattcg cggaatgttg gtccatgcgt tatcagacca
    56161 ggcccgtgag ttttatctgc gagtggggtt tgaaccatcg cctgtcgatt ctatgatact
    56221 gatggcaaca ttggctgatt tgcaggaatg tcttaaatag ttcgttatgt ctcatgcaaa
    56281 aaaatgcccg ccccggcgat cccagagcag gcagacaaaa catacccagt ttcatgatat
    56341 gttttaaagc gaaattattt gttgagctgg aagagcgaca atccctgcat atcggtaaat
    56401 gctttatacg acgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat agttgcattc
    56461 cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct
    56521 aatgaatcca gcgactccag ttcgttcagc tgcgtgccta attccgcgcg aacagtcagc
    56581 acattgttca gcgagttttt cagtccgcgg ttggttttat ccagtgccgc agcggcggtt
    56641 tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc gctatccagc
    56701 atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc gttgctggta
    56761 atactgtcga aaattttgtc acccgtgtgc cctatcacca tcgaacgcga agcatcgacc
    56821 tgttgtttaa tactttctgc tccgccttcg tattttccat ccgcctcgct aaacggtgcg
    56881 gattctgttt tataaccggc aaaaatgtag cgcccgttac cgtcagtggt gttcgccaga
    56941 ttcagcaact ggtcacgaag cccctgaata tccgtagcca gcgaggcccg gtcatcgtca
    57001 ctcaaggtgc cattgctggc gtagacaatt ttttcctgag cattctggat agcggtggtg
    57061 acctggctaa gtacactctc ttccagtgac actttttgag tggcgaaagt acgcgccagc
    57121 gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa cagcttgtga tgcagcaatg
    57181 ggatcatctg aagggttaac gactcgctta cccgtcgaca tctgttcgcc gtacttcatc
    57241 cattctgcct gagaattggt gataccacgc atgttttgct ggtacatcat ctgggtactg
    57301 aaacgcatct tatctcccct tagcgaatgt taatcagcgc atcaaaaatc gcgtttgccg
    57361 tctgcagaac ctgcgcattc gccaggtaat actgctgaaa acgttgcaga tttccgtact
    57421 cctcatcgag attgacaccg gaaatcgact gctgctgatt ggaaagctgc gtcaccacat
    57481 taccttgcgt ggtgctactg gttttcaacg tcgcggtttt attaccgata tcactcacta
    57541 acgaggcata agcgtcgtta aaggatttcg caccgcccac cgttttactg ttgctttgca
    57601 gatcgagcag ggcctgaccg ttgcggttgt cgctatcacc tgtatcttct gcgctcgcca
    57661 tggcgatttt cgcttcgtcg gtgattaata catccatgtt gacgatggcg tcacttaccg
    57721 gtttcagcgt gaagctgtcg ttaacggcag gcgttcctgt aaacgtcaac tccagaccat
    57781 caaatgccac tttaccgctg gcatccggcg tcaccgtaaa agtggtatta ctggcaaggc
    57841 gggtgacctg ccactgatta ttatcgaacg agattttgta atctgtcgcc agtaccgcgg
    57901 aggcatcagt taccgttgca ccgatcgcaa cgtcaccttt gtttttcgtg ttttgcagaa
    57961 ccgcgggctt accgatagca aagaaatctt caccggcatc gccgttagca tcaaatccgg
    58021 ctttgtgttg ggtgttgaaa gcctcggcaa atgccagcgc cagttgtcca agcgtattac
    58081 gcgtctggtc cagatcctga gaacggaatg tcagaatgcc gcccagcgac ccggtattca
    58141 gtaatttctc cgggatctca atattgcctg ccgtcccatc aacataagcg acagtcgtac
    58201 gagaagggtc agcgctggaa ggaactgccg ccagttgccg agccgtactt ccctgaacca
    58261 gtgagtaacc attggccatc gtgatgttat aagtgccacc atcctgaacg ctgacttcta
    58321 caccaacaat ctggtttaat tcgctcacca gttgatcgcg ttgatccagc agattgttag
    58381 gtgacgcccc tgcccccacg cctgtcaggc gggagatttg atcgttcagg ctggcgattt
    58441 gtttagcgta attgttgatc tgatcaacgc tggcacctat cgcgatattg acctgtttgt
    58501 cctggtcgcg cagatattga tcggtggttt taaactgatt caccaaccct tctgatttcc
    58561 caatcagcgc ctggcgcgct gccggatctt ccgcgttact caccagcgtt tgcaggctgg
    58621 tgaagaaatc ctgcatctgt gttgccagcg aagaggtact ggtggagagc atattgtcga
    58681 ttttcgacat ctgctcatag cgggcggtca ggccgctact ttgcgtctgc gcagcacgta
    58741 actggttggt aataaacgca tcatactcac gctgcacacc agaaacgtag acgccattgc
    58801 caacccagcc gccagcgccc aacgtgctat tggcctgcgc cataatagtg gtttggcggg
    58861 tatatccggc aacgttatag ctggagatat tattacttgc cgtatttaac gccgcctggg
    58921 ccgcgttcag tccgctcatg gcgttattga tcaagctgga catggaggtt ccttataagc
    58981 cttcaattac agagtattat cggcagcgcc ccgccggact tgagttattc agaacagatt
    59041 atcaatgttc atgctgtagg ttttgctcac cttgtcgctt atcgatttca tctgctgaat
    59101 catgctggtg agtttgcggg catagtgagg atcggtggca tagcccgcgt cctgtagggc
    59161 ctgcgccccc tgctccgcac tcgcggcggt cgtcacggcg gcgtagcgcg ggttacgcgt
    59221 taacagccca acgtaatccg acaatgcttc cagatacgag ctgtagaccc gaaacttcgc
    59281 ttttactttc ttcgcttcgc cattttcata ttcagtcgtg gtgatttcag tgactggccc
    59341 tttccagttg ccagaggctt tgacgccaaa caggttatag ctcggctcgc cgttttcacg
    59401 gcggatttgc cgttgtcccc agccagattc cagcgccgcc tgagcgagga tcaaatgatg
    59461 tggcacaccg ctttgctggc ttgccagttg ggcgggcaac gagagttgcg cgaggaatgc
    59521 tttactgtca cccggcagcg aatcatcgta gttacgtggt acggcctttt gcaccagctg
    59581 cgaaagcgtc tgattttgat aacgcaccac ggtttcgagc gggaatttca tcggtgctgc
    59641 tggcgtggac tcctctggca atggttgttc tggcgtcatc tgtttaacca tcatctctgc
    59701 cagccccaga cctttgcccg ccgtcatctg ttgggcaatc tgctggtcat acatactggt
    59761 atacaggcga gtgtgctcgc tgctgaacag gccatctttt ggtaaagcgt cgcgcatgct
    59821 tttcaacatc atctgcacga acatcccttc cacctggcgg gccaccggac ggatatttgc
    59881 cgccggatct tcgcccgctt tcgccttaag ttcgttgagc gattgtgcat cccaggccgc
    59941 acttgccagt agtttgctgt cgctgatcat cagatgattt ccagttttgc ccgcagacat
    60001 cccgcacttt gcattgattg caaaatagac atcagatcca tcggcgtagc gcccagcgca
    60061 ttgagcgcac gcaccacgtt attgaggctg gcgctggaac gtacgctttg cagcgaaccg
    60121 ccgctctggc gtaaatcgat ctgcgtttgt ggagtaacca cagtctgtcc accaccaaac
    60181 ggtgtatctg gctggctgac attggcctgg cgattaactg ttactgagag atttccctgc
    60241 gctaccgcgc agctgtcgag ggtcacttcg cgattcatca ccaccgaacc ggtgcgcgag
    60301 ttaatcacta ctttagcgtc ctgcggggta acattaacct gcatattctg gatatcggca
    60361 aggaaacgga cctgggaact gttgccactc ggtacgcgca cctgaatagt ccgcgcatcc
    60421 aacgcggtgg cgctgccata tccacgcact cggttgatgg tgtcagcgat ttgctgtgcc
    60481 atgctgaaat cttcgtcgtt aagttgcaaa ttaagggtat tcccgacgcc aaactggctg
    60541 ggcaattcac gttcaataac cgcaccattg gtgatccgtc caccgttcag ttggttcacc
    60601 tgaacactgc taccgccagc ggaggcccct gcgccgccaa ccagaatatt gccctgcgcc
    60661 agcgcataca cctgactgtc aacgccctta agcggtgtca tcaataacgt accgccacgc
    60721 agacttttgg catttcccat ggaagaaacc accacgtcaa tggtttgccc ctgacgtcca
    60781 aacggtggaa gtgacgctgt caccataacc gcagcgacgt tttttagctg catattagtg
    60841 cccgtcggaa cggtaattcc cagctgtgag agcatgttat taagcgtttg tgtggtaaac
    60901 ggcgtctgag ttgtctggtc accagttcca tccagcccca ccaccaggcc atagccaatc
    60961 agtgagtttt gccttacccc ctgaacactg gtgagatcgc gaatacgctc agcctgagcc
    61021 gccgtcgtaa ccagtagaag aattaatgca gagagaaatt taatcaccac agcctcactt
    61081 acattggaga caggttaaga aagaaacgct gcaaccagcc catattttgc gcttcgttaa
    61141 tgtagccatt gcctacgtat tcaatgcgcg catccgccac ctgagtagac ggtacggtat
    61201 tgctgccgct gatagtgcgt ggattaacca cgccagagaa gcgaataaat tcggtaccct
    61261 gattaatggc gatctgtttt tcacccacca catgcaggtt gccgttaacc agtacctggt
    61321 caaccgtcac cgtcaacgtg ccgctaaagg tattactggc attggccccg ccttttccgt
    61381 tgaacgtgtt accaccggag gcttcgacat cggcacgagc gttaccaaac agcccctgca
    61441 aatagcgcgg cacagtatca aagccaaaat tagttttacc gtcacggctg gcattcgcag
    61501 aggagctttt gctggcgctg acgttctcct gcaacacgat ggtcagcgta tcgccaatat
    61561 tgcgtggtcg acgatcttca aacagcggtt gatagccata gttaatcggc tgagcagact
    61621 ggaaaataga accgttggcg acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc
    61681 cctgtaccag cggcgtggag ggtatccagg cgcagccggt tagtgaaagc accaacaagc
    61741 tggaaatggc ataagtatgc gcagcgtttt tttgcatggc tatcatcttc aaaaatcagt
    61801 aaaccggtga atctgccacc ggttatgcct tagagttgcg tcagtttttg cagcatctga
    61861 tcggtggtgg ataccgcttt actgttgatt tcgtaagcgc gttgcacctg aatcatattg
    61921 accagttctt ccgccacgtt gacgttagac gtttcaacat acccttgata cagcagtccc
    61981 gcgccgttca ggcccggcgt gctttcgttc ggtgcaccgg aggattgcgt ttcggtgtag
    62041 aggttttcgc caatactctc cagtccggta tcattcataa aggtggtgag attgagctgc
    62101 ccaacctgaa ccggagctgc ctggccttgt tgtgttacgc tgaccacgcc atcacgaccg
    62161 atggtgatac ttagcgcatt cgccggaatg gtgatcgctg gctgcacctg aaaaccacct
    62221 gccgtcacca gctgcccgtt ctgatccacc tggaaagagc cgtcacgggt ataggctgat
    62281 gagccatctg gcaacatcac ctggaaaaag ccctgccctt taatcgcgac atctttgctg
    62341 ttgttggtct gcgacagatt gccctggctg tgtaagcgtt cagtggcgac cgggcgtaca
    62401 cccgtgccga tttgtaatcc ggatggtaag gtggtttgtt cggaagactg agcacctggc
    62461 tggcgaatgg tttgataaag cagatcttca aataccgcgc gctgacgctt aaaaccgttg
    62521 gtactgacgt ttgccaggtt gttggcaatg acgtccatat tggtttgctg ggcatccagg
    62581 cccgttttgg cgatccataa tgaacttatc ataaaatgtc ctgtagtgga ttagtgacaa
    62641 agtgcatctg tcttcttgcc ggatgcggcg taaacgcctt atccggccta caagagctcg
    62701 cgaaatcaat aaattgcgat tgccccatag acccgataaa cttgcgcatc gggcaactta
    62761 tacttgtcat gtatcctttc aattaactca tcgacagcag ttggttggct cggcctgcgt
    62821 tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg ctggcaatca
    62881 tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaacaccc gacatcacac
    62941 gcaaggttgg atctgcctgc agtaccggcc cacgcgtggt ctgggtttct gcgcttaaac
    63001 gaaaaatgcc gtcgtcaccg cgctggactt cgctgcccgt ggctttcacc agtttcagac
    63061 gccccactgg cgcaaccgta tttgccggat cgcccggatt cagcgccgag attgtgccat
    63121 cggcagcaat agtgatttcc gccccttccg gcacagcaat tggcccagcc tcgcctatca
    63181 ccggatgtcc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg ccattacgcg
    63241 tatacccttc gctaccgtca gcggtctgca cggccagcca gccatcctgc tgcaacgcaa
    63301 cgtccagcgg gcgcgaggtg taatccatct tgccgggcgt catgtctgca cccggcgttg
    63361 acgccgttac cagcgtgcgc gtaggcagtg aaagcccttc caccggcact gcgcgcaagg
    63421 cgtttaactg cgcgcgaaaa ccgggcgttg aggcattggc cagattactg gcggttaccg
    63481 cctgttgatt caacgtctgg ctggctgctc ccatcgcggt ataaattgcg tgatccattg
    63541 agctatcccg tcagcgatta gcgtaagtta accagcgtgt tgaggatctg gtcctgggtt
    63601 ttgatggtct gggcgttaga ctgatagtta cgctgagcaa cgatcatatt gaccagttct
    63661 ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagggtgcc aaagttgccc
    63721 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    63781 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    63841 tgggtttgtt cgttggaata gttgccgaca accgtaccgt catcattgat ttgataactc
    63901 accagatcgc ccggtttgta gccgttctgg gtggttgcca caatgttgtt agcgccggta
    63961 ttttgctgca tggagttgag gaagctcagg ctaaacgtgg cgggttctgc accgttaatt
    64021 gcgccagtgg aaatattcgc tgttggatct gaggtcagaa cgccattggc attaaacacc
    64081 agcgtcattg caggctcagc tgtacctgtt ggatcactgc tatcctgggt gtagacctgc
    64141 cagttattat ccccggtctt cacaaagtag acgctcatgt catgagcatt accctgacta
    64201 tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctagc atcaaatgcg
    64261 ttaacagagg gaagcggatc actggaattc aggttgatct gcatcgacgc cgtggtggta
    64321 gttttcgctg ccatcagggt attcggaatc gaaatattgg tcggattcgc cccttgctga
    64381 atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct gtaaaccttg catattcacc
    64441 aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac cgaaccgttg
    64501 ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc tcgcccggtg
    64561 ttggtggtcg tgccatcggt aaagtcctga gtgataccag caacttttac ccccagtccc
    64621 actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa gccgtaggtg
    64681 gcagagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc gtttaatccg
    64741 ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta aattatctgc
    64801 cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt accgttgttg
    64861 ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag ttgtgtacca
    64921 ccgttactgg cgctaatcgc tacgttgtaa gaaccgttcg gcgcagtggt gccgtcagtc
    64981 aacgtaccgt cccaggtgaa actgtggact ccggcggtca gttcaccaat atcaatggta
    65041 cggacaaccg caccgttttt atcggtgatg gtggccgtca ctttgtctgc cgcctgttgc
    65101 agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc ggttcccgca
    65161 agaacagtgg tgccggggat catcacgccg tgaccgatca ggttactggc ctgtaacgac
    65221 tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt ttcaatcccg
    65281 ctgaccgtgc tgatttgagc caattgcgac gtcagctcgt tgttttccat tggattggtc
    65341 gggtcctggt ttttcagctg cgccaccaac aaagtcagaa aactgctttg taaatctgcg
    65401 gcgttgctgc ccgtgagcga actactactg gtggtgctga cgccggtatt tgtcggatcg
    65461 gtggtggtta ccgcaatgga catagttttc tcctttattg accgagcgta agggttttca
    65521 gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg cgtgacgccg
    65581 acatggtgtt aaccatctct ccgacgacat caacgtttgg cattttcacg tagccctttg
    65641 catccgccag cggattaccc ggttcataaa ccagtttgtc cggggcctga ctttctataa
    65701 catcggcaac ctttacgccg cctgtcgcag cacctggcgc agcgttaacc tgaaatacca
    65761 cctgttttgc ccgatatggc tgtccatcgg gaccggtcac gctatcagca ttcgccagat
    65821 tactggccgc cacgttcagg cgctgggact gagcagttaa cgccgacccg gcgatatcaa
    65881 aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga
    65941 tttgcccgct caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg
    66001 tgcgttcgcg atccatatcg acagtattac catccagcga gggctggtcc ggaatacggt
    66061 attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg aatgtgttgc gttgaggtca
    66121 tcgtcagtgc aaccacgctt gttgcatccc gtccacgttg catgactttt ttaagttcac
    66181 tggcaaaatc gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg
    66241 ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg
    66301 cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attcggccat cagcttaaat
    66361 gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg
    66421 atggtcattg cggacaggta caattcacgt tgtagaaatg gctggggggc gaaaatgctg
    66481 gcaataaaac gtagcgtggc gatcatcgcg atactgttca gtccgttaag tgcggcgagc
    66541 aatctcacat cgcaattgca cacctttttt agcgcccagc tcgcgggggt aagtgatgag
    66601 gttcgtgttt ctattcgtac agcgcccaat ctactaccgc cgtgcgagca gccattgctt
    66661 tcgatgagca ataattcccg cctgtggggc aatgtgaatg tgttggcacg ctgcggtaac
    66721 gacaaacgat atttacaggt taatgtgcag gccacaggaa attatgtggt tgccgcaatg
    66781 cccattgtgc ggggaggaaa gctggaagct ggcaatgtca aactgaaacg cggacggctg
    66841 gacaccttgc caccgcgtac ggtgctggat atcaatcaac ttgttgatgc cgttagcctg
    66901 cgcgatctat cacccgatca acctatccag ttaacccagt ttcgccaggc atggcgggta
    66961 aaagcgggac aacgcgtcaa cgtgatcgcc agcggtgatg ggtttagcgc caacgcagaa
    67021 ggtcaggcgc tgaacaatgc tgccgtcgca cagaatgcgc gggtgcgcat ggtatcggga
    67081 caagtggtca gcggcgttgt tgatgcagat gggaatattc ttataaacct gtaagctgtt
    67141 aaagattacc cgtcccttgc cgataaataa gcaacacatg ataaaagcgc cctcaatgag
    67201 gaataaacca tgagtattga tcgcacttcg cctctgaagc ccgtaagcac cgttcaaccg
    67261 cgcgaaacca ctgacgcgcc ggtaacgaac acccgggcgg caaaaacaac cgcctccacc
    67321 agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat
    67381 atcaatcttg aacgtgtcga agcgttaaaa ctagcgattc gtaacggtga actaaaaatg
    67441 gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt gcagagtaac
    67501 tgatcgtatg acgcgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct
    67561 caaaacggta atggatcaag agcagcaaca tctctctatg gggcagatca acggcagcca
    67621 gttgcaatgg attacagaac aaaaaagctc tctgctggcg acgctggatt acctcgaaca
    67681 gttacgcagg aaagaaccca atgcagcaaa tagcgttgat attagtcaac gctggcagga
    67741 aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga
    67801 aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac
    67861 gctatatgga gcgaacggtc agacctcaac aacccatcgc ggcagtaaaa agatttcgat
    67921 ctgaagatta tctccggcct gcactgcagg ccggaatgcc tttttacacc gtccggcggg
    67981 caaactcttt aactttgaag cccagtaccg ccagtgctgc aaagtacgcg gcaatccccg
    68041 ccagcacgac cgccattaaa cgcagtaaac gccagggcat ggtacccaat gaccactccg
    68101 gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac
    68161 gcaacagaaa cgccatccag ccgggttgcg gggtaaagat tttctgctta cgcaactgcc
    68221 agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccggcat
    68281 gtttcaacgg accgataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg
    68341 caattttcac tggcgtttta atgtcctggc gggaataaaa gccaggagcc aacactttca
    68401 ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg
    68461 catcaaaggc ggtaaattta ccgtactgga acagcgaaac ggttaacgga cctgaaagaa
    68521 tgcccaacgc aaccgcactc ggcagcgcca acaggaaaca aagacgcagc ccccagtcca
    68581 tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca
    68641 aaatggtgcc aagcgccacg cccagtacac cggacggaaa ctccattaag cggtcggcgt
    68701 aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta
    68761 aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca
    68821 ttgctccggc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt
    68881 gcggtagctg atacaccagc tgcaggatgc cgccgaccgt aacagcccac gccagcgcca
    68941 gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatattaa
    69001 gcagtgttgg agcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca
    69061 ccagcgacgc cagggagatc agcaagatat agggaaaggt tatctttagt agctggctgg
    69121 tcagggcaaa tttgtcggca gtgtcagcga agcctggcgc ggtcaccatg atcacccacg
    69181 gtgcagcgag catgccagcg accgtcacaa ccgccagcgc gagtgtcagc aggccagaaa
    69241 cataagagac aaagacccgc gtggcgtctt caccctgctt gcttttatat tctgccagaa
    69301 tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgcaac aagttaggaa
    69361 gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa
    69421 ttgcgtcacg tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca
    69481 gcgattttaa taaattcatc ggtgttctaa tccagaccca cgggcaaaac gcctgcaaag
    69541 caggcgctga aagaggcgaa tagtctacct ggattatggt gaattgctac cgccagatgt
    69601 tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgcccgc
    69661 actgcctgtt cgccggcggt ttgcggaact gtctgatttt gcacgcattc aatgaagtgc
    69721 cgcgcacagc cgacaaatcc acgttgctca agcgtactct gccaaccagg aatcggttta
    69781 tgcactacgc cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc
    69841 gcaccgtcag tcacggcctg cacggtttca cgctggcttc cggcacggcg atgcatacag
    69901 gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg
    69961 tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg
    70021 gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg
    70081 ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac
    70141 tctccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc
    70201 gccgccagtt ccaccaaccg ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca
    70261 cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg
    70321 gaatgcacaa aaaccgcatc gcaactggcg gcaaggctgg ataacgaatc ggcataagga
    70381 atgcgccagc tttcacaaat tggcagggct ttcgcgcgcg taggcgacca ggctccttgt
    70441 aacatccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct
    70501 aatcccacta cgccgatacg taattttttc actttaatct cccagatggg caagcaacga
    70561 atcaagacgc tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gagcctgtaa
    70621 atcaccgtca accgcgttcg acatatccat caccgccggc tgatcttcaa cctcaccact
    70681 gaaaagatgc atgtagcggc tttcacgttt acccggttcg cgggccagac gcaccacaaa
    70741 aggaccatct tcgcgatttg ccagttgttc cagcgtcgac tccacttccg ccatatcgct
    70801 gaattcatac attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg caccacgcaa
    70861 taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga
    70921 attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag
    70981 ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac tcagattcat
    71041 gaccggttca cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg
    71101 gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaatcacgc gagcttcaag
    71161 ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag
    71221 ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat
    71281 agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca
    71341 tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg
    71401 tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat
    71461 agcaggcatg aaaagagcca cgaacaacat tggaaaaatt ggcaacgcca ataatctctt
    71521 tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa
    71581 tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct
    71641 cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac
    71701 gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac
    71761 tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt
    71821 attactatac ctgcgcaacc agaccctgtg aaaagtccag aatgccattt tctgcattat
    71881 tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc
    71941 gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc
    72001 cgggagggga aatgtcccgc gtgtcgcagg ccaggaacct gggtaaatat ttcctgctca
    72061 tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct
    72121 tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgccctcggc ctacgccagt
    72181 ttattcagca aggtctgggt attttcggcg gagcaattgc cgaccgcttt ggtgccaaac
    72241 cgatgattgt taccggtatg ctgatgcgtg ccgccggatt cgccacaatg ggtatcgccc
    72301 acgaaccgtg gctactgtgg ttttcatgcc tgctttcggg actcggcggc acgttgtttg
    72361 atcctccgcg ttcggcgctg gtggtgaaat taatccgtcc acagcagcgt ggtcgttttt
    72421 tctcgctgtt gatgatgcag gacagtgccg gtgcggtcat tggcgcattg ttggggagct
    72481 ggctgttgca atacgacttt cgcctggtct gcgccacagg ggcagttttg tttgtgctgt
    72541 gtgcagcgtt taatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg
    72601 ttcgcgaagg catgacccgc gtgatgcgcg acaagcgttt tgtcacctat gtactgacgc
    72661 tggcgggtta ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg
    72721 acgtggcggg cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt
    72781 taacgttgct ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaacaccggt
    72841 tgatggctgg gctattgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggtc
    72901 tgcaacaact tttcaccctg atttgtctgt tttatatcgg gtcaatcatt gccgagcctg
    72961 cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt
    73021 ttagccgtct gggtctggcg attggcggcg ctattggtta catcggtggc ggctggctgt
    73081 ttgacctggg caaatcggcg caccagccag agcttccgtg gatgatgctg ggcattattg
    73141 gcatcttcac tttccttgcg ctgggttggc agtttagcca gaaacgcgcc gcgcgtcgtt
    73201 tgctggaacg cgacgcctga tttgatactc ccccctgact cctccatact gaaagtgaac
    73261 tgacagccaa atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc
    73321 tcaaagcccg catgattttc ggcctgaaaa atatccccat cgaattacat gttctgctca
    73381 acgacgacgc agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa
    73441 aagatgacag ccgctatatg ccagaaagca tggacatcgt tcactatgtc gataaactcg
    73501 acggcaaacc gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg
    73561 tcaatggcta cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt
    73621 tttctactcc tgccgcgcgc aaatatttcg tcgacaagaa agaggctagc gcgggtaatt
    73681 ttgccgacct gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttacgtg
    73741 cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata
    73801 ttcagctatt tccgctcctg cgtaatctga cgctggtagc cggaatcaac tggccaagcc
    73861 gcgttgctga ttaccgcgat aatatggcga aacagacaca aatcaatttg ttatcatcaa
    73921 tggcgattta atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag
    73981 ttttttctgc tggcgcgttt atcgctggtg acacatgctg cgttgtgtca ggatattgac
    74041 gcagatttca tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc
    74101 agtggcctgc tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt
    74161 aaccagtcgc ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct
    74221 gacgcccata ttgttctgac aaacctgacc agccaaattg gtcgcgaaga gccgaataag
    74281 gttaccctaa ccggagacgc caatctggac atgaactccc tgttcggtag ccagaaagcg
    74341 accatgaaac tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg cgctatcttc
    74401 ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg
    74461 caaacgttgc ttccctattt aaaccaggca ttacgcaatt actttaacca gcaacctgct
    74521 tacgtcctgc gcgaagatgg cagccagggc gaagcaatgg cgaagaaact ggcgaaaggc
    74581 attgaagtga agccaggcga aattgtcatt ccgtttactg attaatcacg agggcgcatt
    74641 cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct tatcctttgt gtccggcaaa
    74701 aacatccctt cagccggagc atagagatta atgactgcac catcccaggt attaaagatc
    74761 cgccgcccag acgactggca ccttcacctc cgcgatggcg acatgttaaa aactgtcgtg
    74821 ccgtatacca gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg
    74881 accaccgttg aggctgccgt ggcttatcgc cagcgtattc ttgacgccgt acctgccggg
    74941 cacgatttca ccccgctgat gacctgttat ttgacagatt cactagaccc taatgagctt
    75001 gagcgtggat ttaacgaagg cgtgttcacc gctgccaaac tttacccggc aaacgcaacc
    75061 actaactcca gtcacggcgt gacgtcagtt gacgcaatca tgccggtact tgagcgcatg
    75121 gaaaaaatcg gtatgccgtt actggtgcat ggtgaagtga cacatgcaga tatcgacatt
    75181 tttgatcgtg aagcgcgctt tattgaaagc gtgatggaac cgctacgcca gcgtctgact
    75241 gcgctgaaag tcgtttttga gcacatcacc accaaagacg ctgccgacta tgtccgtgac
    75301 ggaaatgaac ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat
    75361 atgctggttg gtggcgtgcg tccgcacctg tattgtctac ccatcctcaa acgcaatatc
    75421 caccaacagg cattgcgtga actggtcgcc agcggtttta atcgcgtatt cctcggtacg
    75481 gattctgcgc cgcatgcacg tcatcgcaaa gagagcagct gcggctgcgc gggctgcttc
    75541 aacgccccaa ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag
    75601 cactttgaag cattctgttc tgtaaacggc ccacagttct atggcttgcc ggtcaacgac
    75661 acattcatcg aactggtccg caaagagcaa caggttgctg aaagcatcgc attgactgat
    75721 gacacgctgg tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa
    75781 tcgtagcttc ctgttgtcat taggttattt taactgtata aataaccagt atattcaaca
    75841 gggggctatt atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctggggc
    75901 tattgacgcc ctggctggcg aactttcccg ccgtattcag tatgcgtttc ctgataatga
    75961 aggccacgta tcggtacgtt atgccgcagc gaataattta tcggttattg gcgcgacaaa
    76021 agaagataaa cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg
    76081 gtttgtcagc gaataatatg cagtgatttt tgtttttgcc gggtcgcccc ggctttttta
    76141 ttttacttgc gaattgtgag tacaaaattt ctacacttcc tcaaaataat tgcacaatat
    76201 gctaaaaaaa tatgaatgac atgctgtttt attgttcata taaatcttaa aagcaaaaat
    76261 ttatctgttc ccccctttaa aaattcggtg aataccctta cttattggta tactgaagtt
    76321 gcttcagata aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg aattaggggg
    76381 ccatgatgga aaaaaataat gaagtcattc agactcatcc gctcgtaggg tgggacatca
    76441 gcaccgttga tagctatgat gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt
    76501 ccgagcagga agggactgaa gttggtcaga cgctctggtt aaccactgat gttgccagac
    76561 aatttatttc gatattagaa gcaggaatcg ccaaaattga atccggtgat ttcccggtaa
    76621 acgagtatcg gcgtcattaa catgacatga ccatccaccc aaaaggcacc tcaccggtgc
    76681 ctttaccatt tctgggttgt atatctgcat tcagttaatt actctgctta aaccatgaga
    76741 gcagagagaa aaaaatgaaa tacgatctca tcattattgg cagcggttcc gtaggcgctg
    76801 ccgccgggta ttatgcaacc cgtgccggtt taaacgtgct aatgaccgac gcccatatgc
    76861 caccgcatca acacggcagc caccacggcg atacgcgatt aattcgccat gcttatggtg
    76921 aaggcgaaaa gtatgtcccg ctggttctcc gcgcgcaaac gctgtgggat gaactctccc
    76981 gccacaacga agaagatccc atttttgtac gctctggcgt cattaacctt ggcccggctg
    77041 attccacatt tctcgccaac gtcgcccaca gcgccgaaca atggcaactc aacgttgaaa
    77101 agctcgatgc gcaagggatt atggcccgct ggccagaaat acgcgtcccg gacaactaca
    77161 tcggcttatt tgagactgat tccggctttt tacgcagcga actggcgatt aaaacctgga
    77221 tccaactggc gaaggaagcg ggctgtgcgc agctgttcaa ctgcccggtc accgcaattc
    77281 gtcatgacga tgatggcgta actattgaaa cggctgacgg tgagtatcag gcgaaaaaag
    77341 cgattgtctg cgcgggaaca tgggtaaaag acctgctccc ggagttgcct gtccagcccg
    77401 tacgcaaagt atttgcctgg tatcaggccg atgggcgcta tagcgtgaag aataaattcc
    77461 cggcgtttac cggtgaactt cccaatggcg atcaatatta tggttttccg gcagaaaacg
    77521 acgcgttgaa gattggcaaa cataacggag gccaggttat ccattcagcg gatgaacgtg
    77581 ttccgtttgc ggaagtggtc agcgatggtt cggaagcctt cccgttcttg cgcaatgtat
    77641 tgccgggtat cggttgctgc ctgtacggcg ccgcctgcac ctatgataat tcgcctgacg
    77701 aagattttat tatcgatacc ctacccggcc acgataatac actgctcatt accggcctga
    77761 gtgggcacgg ttttaaattt gcgtcagttt taggggaaat agctgccgat tttgcgcaag
    77821 acaaaaaaag cgattttgat ttgacgccat tcaggctttc ccgcttccaa taatcatcat
    77881 tacggcctcc agaatgaggc cgtttttaca ggagtattta atgcgtcggc tgttgcacta
    77941 tctcatcaat aatattcgcg agcatctgat gctttatctt tttctctggg gattgttggc
    78001 aattatggat ctgatctatg tgtattattt ttagttttcc gcactgacat aaactgacaa
    78061 atatttaatc tccctttcat ttagcgtgaa aaattgaatg agattattcc tgc
//
LOCUS       DAZKJR010000004        71558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKJR010000004 DAZKJR010000000
VERSION     DAZKJR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.004"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..212
                     /gene="ypdI"
                     /locus_tag="ACHOWS_000253"
     CDS             <1..212
                     /gene="ypdI"
                     /locus_tag="ACHOWS_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFQ0045905.1"
                     /translation="AFSISGGRSETSYKETEKTSVMTTTHSTKLQPSQAILFKLREDA
                     PPLNLTEDITPPFSTKANYLIHPVR"
     gene            complement(289..531)
                     /gene="yfdY"
                     /locus_tag="ACHOWS_000254"
     CDS             complement(289..531)
                     /gene="yfdY"
                     /locus_tag="ACHOWS_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFQ0045906.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            884..1804
                     /gene="lpxP"
                     /locus_tag="ACHOWS_000255"
     CDS             884..1804
                     /gene="lpxP"
                     /locus_tag="ACHOWS_000255"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFQ0045907.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2160..2231
                     /gene="ypdK"
                     /locus_tag="ACHOWS_000256"
     CDS             2160..2231
                     /gene="ypdK"
                     /locus_tag="ACHOWS_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFQ0045908.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2296..3534)
                     /gene="alaC"
                     /locus_tag="ACHOWS_000257"
     CDS             complement(2296..3534)
                     /gene="alaC"
                     /locus_tag="ACHOWS_000257"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFQ0045909.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGIEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3911..5608
                     /gene="ypdA"
                     /locus_tag="ACHOWS_000258"
     CDS             3911..5608
                     /gene="ypdA"
                     /locus_tag="ACHOWS_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFQ0045910.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5623..6357
                     /gene="ypdB"
                     /locus_tag="ACHOWS_000259"
     CDS             5623..6357
                     /gene="ypdB"
                     /locus_tag="ACHOWS_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFQ0045911.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6370..7227
                     /gene="ypdC"
                     /locus_tag="ACHOWS_000260"
     CDS             6370..7227
                     /gene="ypdC"
                     /locus_tag="ACHOWS_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0045912.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPVRQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7230..9725)
                     /gene="ptsP"
                     /locus_tag="ACHOWS_000261"
     CDS             complement(7230..9725)
                     /gene="ptsP"
                     /locus_tag="ACHOWS_000261"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0045913.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDGCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLNLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQMLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9750..10787)
                     /gene="ypdE"
                     /locus_tag="ACHOWS_000262"
     CDS             complement(9750..10787)
                     /gene="ypdE"
                     /locus_tag="ACHOWS_000262"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0045914.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSTIRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10787..11872)
                     /gene="ypdF"
                     /locus_tag="ACHOWS_000263"
     CDS             complement(10787..11872)
                     /gene="ypdF"
                     /locus_tag="ACHOWS_000263"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0045915.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVICR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIFADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQLKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGVEVLYAMPKTVLLTGEA"
     gene            complement(11887..13134)
                     /gene="fryC"
                     /locus_tag="ACHOWS_000264"
     CDS             complement(11887..13134)
                     /gene="fryC"
                     /locus_tag="ACHOWS_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0045916.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13156..13482)
                     /gene="fryB"
                     /locus_tag="ACHOWS_000265"
     CDS             complement(13156..13482)
                     /gene="fryB"
                     /locus_tag="ACHOWS_000265"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0045917.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13701..14666)
                     /gene="glk"
                     /locus_tag="ACHOWS_000266"
     CDS             complement(13701..14666)
                     /gene="glk"
                     /locus_tag="ACHOWS_000266"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFQ0045918.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKIEVQDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14870..16126
                     /gene="yfeO"
                     /locus_tag="ACHOWS_000267"
     CDS             14870..16126
                     /gene="yfeO"
                     /locus_tag="ACHOWS_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFQ0045919.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16241..16567
                     /gene="ypeC"
                     /locus_tag="ACHOWS_000268"
     CDS             16241..16567
                     /gene="ypeC"
                     /locus_tag="ACHOWS_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFQ0045920.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16708..17946)
                     /gene="mntH"
                     /locus_tag="ACHOWS_000269"
     CDS             complement(16708..17946)
                     /gene="mntH"
                     /locus_tag="ACHOWS_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0045921.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18282..19484
                     /gene="nupC"
                     /locus_tag="ACHOWS_000270"
     CDS             18282..19484
                     /gene="nupC"
                     /locus_tag="ACHOWS_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFQ0045922.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYSMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19533..21722)
                     /gene="pdeA"
                     /locus_tag="ACHOWS_000271"
     CDS             complement(19533..21722)
                     /gene="pdeA"
                     /locus_tag="ACHOWS_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFQ0045923.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLARMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(21930..22005)
                     /locus_tag="ACHOWS_000272"
     tRNA            complement(21930..22005)
                     /locus_tag="ACHOWS_000272"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21970..21972),aa:Ala,seq:ggc)
     gene            complement(22045..22120)
                     /locus_tag="ACHOWS_000273"
     tRNA            complement(22045..22120)
                     /locus_tag="ACHOWS_000273"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22085..22087),aa:Ala,seq:ggc)
     gene            22341..22700
                     /gene="yfeC"
                     /locus_tag="ACHOWS_000274"
     CDS             22341..22700
                     /gene="yfeC"
                     /locus_tag="ACHOWS_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFQ0045924.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22702..23094
                     /gene="yfeD"
                     /locus_tag="ACHOWS_000275"
     CDS             22702..23094
                     /gene="yfeD"
                     /locus_tag="ACHOWS_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFQ0045925.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(23145..24560)
                     /gene="gltX"
                     /locus_tag="ACHOWS_000276"
     CDS             complement(23145..24560)
                     /gene="gltX"
                     /locus_tag="ACHOWS_000276"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0045926.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            24819..24894
                     /locus_tag="ACHOWS_000277"
     tRNA            24819..24894
                     /locus_tag="ACHOWS_000277"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24852..24854,aa:Val,seq:tac)
     gene            24939..25014
                     /locus_tag="ACHOWS_000278"
     tRNA            24939..25014
                     /locus_tag="ACHOWS_000278"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24972..24974,aa:Val,seq:tac)
     gene            25061..25136
                     /locus_tag="ACHOWS_000279"
     tRNA            25061..25136
                     /locus_tag="ACHOWS_000279"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25094..25096,aa:Val,seq:tac)
     gene            25141..25216
                     /locus_tag="ACHOWS_000280"
     tRNA            25141..25216
                     /locus_tag="ACHOWS_000280"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25174..25176,aa:Lys,seq:ttt)
     gene            complement(25327..26211)
                     /gene="xapR"
                     /locus_tag="ACHOWS_000281"
     CDS             complement(25327..26211)
                     /gene="xapR"
                     /locus_tag="ACHOWS_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFQ0045927.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(26252..26410)
                     /locus_tag="ACHOWS_000282"
     CDS             complement(26252..26410)
                     /locus_tag="ACHOWS_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFQ0045928.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(26463..27719)
                     /gene="xapB"
                     /locus_tag="ACHOWS_000283"
     CDS             complement(26463..27719)
                     /gene="xapB"
                     /locus_tag="ACHOWS_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFQ0045929.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(27780..28613)
                     /gene="xapA"
                     /locus_tag="ACHOWS_000284"
     CDS             complement(27780..28613)
                     /gene="xapA"
                     /locus_tag="ACHOWS_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFQ0045930.1"
                     /translation="MSQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            28861..29625
                     /gene="yfeN"
                     /locus_tag="ACHOWS_000285"
     CDS             28861..29625
                     /gene="yfeN"
                     /locus_tag="ACHOWS_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFQ0045931.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(29663..30589)
                     /gene="yfeR"
                     /locus_tag="ACHOWS_000286"
     CDS             complement(29663..30589)
                     /gene="yfeR"
                     /locus_tag="ACHOWS_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0045932.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWEVVRGQGRALMAGREGDPLYQI"
     gene            30679..31677
                     /gene="yfeH"
                     /locus_tag="ACHOWS_000287"
     CDS             30679..31677
                     /gene="yfeH"
                     /locus_tag="ACHOWS_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFQ0045933.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(31674..31892)
                     /gene="ypeB"
                     /locus_tag="ACHOWS_000288"
     CDS             complement(31674..31892)
                     /gene="ypeB"
                     /locus_tag="ACHOWS_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFQ0045934.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(31894..33909)
                     /gene="ligA"
                     /locus_tag="ACHOWS_000289"
     CDS             complement(31894..33909)
                     /gene="ligA"
                     /locus_tag="ACHOWS_000289"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0045935.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHEVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAVKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(33980..34978)
                     /gene="zipA"
                     /locus_tag="ACHOWS_000290"
     CDS             complement(33980..34978)
                     /gene="zipA"
                     /locus_tag="ACHOWS_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFQ0045936.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQLVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            35208..35969
                     /gene="cysZ"
                     /locus_tag="ACHOWS_000291"
     CDS             35208..35969
                     /gene="cysZ"
                     /locus_tag="ACHOWS_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFQ0045937.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36154..37125
                     /gene="cysK"
                     /locus_tag="ACHOWS_000292"
     CDS             36154..37125
                     /gene="cysK"
                     /locus_tag="ACHOWS_000292"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0045938.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            37509..37766
                     /gene="ptsH"
                     /locus_tag="ACHOWS_000293"
     CDS             37509..37766
                     /gene="ptsH"
                     /locus_tag="ACHOWS_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFQ0045939.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            37811..39538
                     /gene="ptsI"
                     /locus_tag="ACHOWS_000294"
     CDS             37811..39538
                     /gene="ptsI"
                     /locus_tag="ACHOWS_000294"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFQ0045940.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     TDLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            39579..40088
                     /gene="crr"
                     /locus_tag="ACHOWS_000295"
     CDS             39579..40088
                     /gene="crr"
                     /locus_tag="ACHOWS_000295"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0045941.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(40130..40981)
                     /gene="pdxK"
                     /locus_tag="ACHOWS_000296"
                     /gene_synonym="thiJ"
     CDS             complement(40130..40981)
                     /gene="pdxK"
                     /locus_tag="ACHOWS_000296"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFQ0045942.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            41086..41454
                     /gene="yfeK"
                     /locus_tag="ACHOWS_000297"
     CDS             41086..41454
                     /gene="yfeK"
                     /locus_tag="ACHOWS_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFQ0045943.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDDHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(41457..42368)
                     /gene="cysM"
                     /locus_tag="ACHOWS_000298"
     CDS             complement(41457..42368)
                     /gene="cysM"
                     /locus_tag="ACHOWS_000298"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFQ0045944.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MLAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPFAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(42502..43599)
                     /gene="cysA"
                     /locus_tag="ACHOWS_000299"
     CDS             complement(42502..43599)
                     /gene="cysA"
                     /locus_tag="ACHOWS_000299"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFQ0045945.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(43589..44464)
                     /gene="cysW"
                     /locus_tag="ACHOWS_000300"
     CDS             complement(43589..44464)
                     /gene="cysW"
                     /locus_tag="ACHOWS_000300"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFQ0045946.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(44464..45297)
                     /gene="cysT"
                     /locus_tag="ACHOWS_000301"
     CDS             complement(44464..45297)
                     /gene="cysT"
                     /locus_tag="ACHOWS_000301"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFQ0045947.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(45297..46313)
                     /gene="cysP"
                     /locus_tag="ACHOWS_000302"
     CDS             complement(45297..46313)
                     /gene="cysP"
                     /locus_tag="ACHOWS_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFQ0045948.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(46471..47262)
                     /gene="ucpA"
                     /locus_tag="ACHOWS_000303"
     CDS             complement(46471..47262)
                     /gene="ucpA"
                     /locus_tag="ACHOWS_000303"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFQ0045949.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(47391..48248)
                     /gene="murR"
                     /locus_tag="ACHOWS_000304"
     CDS             complement(47391..48248)
                     /gene="murR"
                     /locus_tag="ACHOWS_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFQ0045950.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKEQALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSELVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            48412..49308
                     /gene="murQ"
                     /locus_tag="ACHOWS_000305"
     CDS             48412..49308
                     /gene="murQ"
                     /locus_tag="ACHOWS_000305"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0045951.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSQEGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGPEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            49312..50736
                     /gene="murP"
                     /locus_tag="ACHOWS_000306"
     CDS             49312..50736
                     /gene="murP"
                     /locus_tag="ACHOWS_000306"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFQ0045952.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            50741..52042
                     /gene="pbp4b"
                     /locus_tag="ACHOWS_000307"
     CDS             50741..52042
                     /gene="pbp4b"
                     /locus_tag="ACHOWS_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFQ0045953.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSALMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPCSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSDTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(52100..52999)
                     /gene="yfeX"
                     /locus_tag="ACHOWS_000308"
     CDS             complement(52100..52999)
                     /gene="yfeX"
                     /locus_tag="ACHOWS_000308"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFQ0045954.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSIHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(53095..53670)
                     /gene="yfeY"
                     /locus_tag="ACHOWS_000309"
     CDS             complement(53095..53670)
                     /gene="yfeY"
                     /locus_tag="ACHOWS_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFQ0045955.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(53731..54180)
                     /gene="yfeZ"
                     /locus_tag="ACHOWS_000310"
     CDS             complement(53731..54180)
                     /gene="yfeZ"
                     /locus_tag="ACHOWS_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HFQ0045956.1"
                     /translation="MKSTEFHPVHYDAYGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(54167..54592)
                     /gene="ypeA"
                     /locus_tag="ACHOWS_000311"
     CDS             complement(54167..54592)
                     /gene="ypeA"
                     /locus_tag="ACHOWS_000311"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFQ0045957.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            54806..55675
                     /gene="amiA"
                     /locus_tag="ACHOWS_000312"
     CDS             54806..55675
                     /gene="amiA"
                     /locus_tag="ACHOWS_000312"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFQ0045958.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            55679..56578
                     /gene="hemF"
                     /locus_tag="ACHOWS_000313"
     CDS             55679..56578
                     /gene="hemF"
                     /locus_tag="ACHOWS_000313"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFQ0045959.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGTEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEDGSPEAALSEFIKVRDWV"
     gene            complement(56512..57636)
                     /gene="eutR"
                     /locus_tag="ACHOWS_000314"
     CDS             complement(56512..57636)
                     /gene="eutR"
                     /locus_tag="ACHOWS_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFQ0045960.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSSG"
     gene            complement(57682..58182)
                     /gene="eutK"
                     /locus_tag="ACHOWS_000315"
     CDS             complement(57682..58182)
                     /gene="eutK"
                     /locus_tag="ACHOWS_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFQ0045961.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(58195..58854)
                     /gene="eutL"
                     /locus_tag="ACHOWS_000316"
     CDS             complement(58195..58854)
                     /gene="eutL"
                     /locus_tag="ACHOWS_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFQ0045962.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(58864..59751)
                     /gene="eutC"
                     /locus_tag="ACHOWS_000317"
     CDS             complement(58864..59751)
                     /gene="eutC"
                     /locus_tag="ACHOWS_000317"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFQ0045963.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(59772..61133)
                     /gene="eutB"
                     /locus_tag="ACHOWS_000318"
     CDS             complement(59772..61133)
                     /gene="eutB"
                     /locus_tag="ACHOWS_000318"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFQ0045964.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(61145..62548)
                     /gene="eutA"
                     /locus_tag="ACHOWS_000319"
     CDS             complement(61145..62548)
                     /gene="eutA"
                     /locus_tag="ACHOWS_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFQ0045965.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTEARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPVGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDEKDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(62545..63771)
                     /gene="eutH"
                     /locus_tag="ACHOWS_000320"
     CDS             complement(62545..63771)
                     /gene="eutH"
                     /locus_tag="ACHOWS_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFQ0045966.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(63988..65175)
                     /gene="eutG"
                     /locus_tag="ACHOWS_000321"
     CDS             complement(63988..65175)
                     /gene="eutG"
                     /locus_tag="ACHOWS_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFQ0045967.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSLVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(65165..66001)
                     /gene="eutJ"
                     /locus_tag="ACHOWS_000322"
     CDS             complement(65165..66001)
                     /gene="eutJ"
                     /locus_tag="ACHOWS_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFQ0045968.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKTEGIY
                     AK"
     gene            complement(66012..67415)
                     /gene="eutE"
                     /locus_tag="ACHOWS_000323"
     CDS             complement(66012..67415)
                     /gene="eutE"
                     /locus_tag="ACHOWS_000323"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFQ0045969.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(67427..67714)
                     /gene="eutN"
                     /locus_tag="ACHOWS_000324"
     CDS             complement(67427..67714)
                     /gene="eutN"
                     /locus_tag="ACHOWS_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFQ0045970.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(67821..68114)
                     /gene="eutM"
                     /locus_tag="ACHOWS_000325"
     CDS             complement(67821..68114)
                     /gene="eutM"
                     /locus_tag="ACHOWS_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFQ0045971.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(68153..69169)
                     /gene="pta"
                     /locus_tag="ACHOWS_000326"
     CDS             complement(68153..69169)
                     /gene="pta"
                     /locus_tag="ACHOWS_000326"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0045972.1"
                     /translation="MIIERCRKLALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFALRWLARAGEKTPPDALDKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVQRQTEVNRESSLQ
                     TLVE"
     gene            complement(69166..69969)
                     /gene="eutT"
                     /locus_tag="ACHOWS_000327"
     CDS             complement(69166..69969)
                     /gene="eutT"
                     /locus_tag="ACHOWS_000327"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFQ0045973.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQSLTVKQIQQRLGETQ"
     gene            complement(69966..70667)
                     /gene="eutQ"
                     /locus_tag="ACHOWS_000328"
     CDS             complement(69966..70667)
                     /gene="eutQ"
                     /locus_tag="ACHOWS_000328"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFQ0045974.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(70642..71121)
                     /gene="eutP"
                     /locus_tag="ACHOWS_000329"
     CDS             complement(70642..71121)
                     /gene="eutP"
                     /locus_tag="ACHOWS_000329"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFQ0045975.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(71134..71469)
                     /gene="eutS"
                     /locus_tag="ACHOWS_000330"
     CDS             complement(71134..71469)
                     /gene="eutS"
                     /locus_tag="ACHOWS_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFQ0045976.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        17157 a        19654 c        17946 g        16801 t
ORIGIN      
        1 ttgcattttc catttctggt ggtagaagtg agacgagcta taaagagaca gaaaaaacgt
       61 cagtgatgac gacaactcat tctacaaaac ttcagccatc acaggcgatt ttgtttaagt
      121 tgagagaaga tgcgccacca ttaaatctca cagaagacat tacgccccct ttttcgacaa
      181 aggcgaatta tcttattcat cctgtgcgat agcgtctgtc acctggcagg tgaacaatca
      241 tgcctgatgc tgatgtgaac acctgcccaa atgaaccatc ctgacccact agcgcctgcc
      301 ataccccacc ataaagcgaa tcatcaagcc acaagccggg gcaagaaaca taaatatccc
      361 cgtcactagt tcattaccgc cagttaacca catggtgaag tagtaaatga gtgcagcgag
      421 gatcaccatg ccgaagaaag aagagaccgc cgagaccacg ttcagtacct gaccgatgta
      481 tccgcttacg cagacaatac acagggcgag aaacatccat aaataaatca tagctcaacc
      541 tcctgccgcg ctaaaaaacc tgtaacttca gtcactttaa tagtaagaca atccttaacc
      601 tctggcaatt tttgctaagc atcaatgaaa acagcacgtt aaatgcaacg ctattgcgtg
      661 cggaaaattt tttactttgc acgattaata atcagtagct gaaagcagtc agccagtgat
      721 aaagtagagc caccgcattc atgctcctcg tggttatgtc ctgactagtc tttacactct
      781 ttacaggaac cattgtcgta catgatggcc caaccaattg agtgtaccgc tgtcgacatg
      841 gaatcttctg cgatacaaca attcgtatct acagaaggta actatgtttc cacaatgcaa
      901 attttcccgc gagtttctac atcctcgcta ctggctcaca tggtttgggc ttggtgtact
      961 ctggctttgg gtacagcttc cttatcctgt tctctgcttt ctcggcacgc gtattggcgc
     1021 aatggcgcga ccattcctga aacggcgtga atctatcgcc cgtaaaaacc tggaactttg
     1081 tttcccacag cattctgcgg aagaacgcga gaagatgatt gccgaaaact ttcgttcact
     1141 cggcatggcg ctggtagaaa ccggcatggc gtggttctgg cccgacagtc gcgtacgtaa
     1201 atggtttgat gttgaagggt tggataacct taaacgcgca caaatgcaaa atcgcggcgt
     1261 aatggttgtc ggcgtccatt ttatgtcgct ggaactgggc ggccgcgtga tgggactgtg
     1321 ccaaccaatg atggctacct atcgtccaca taataatcag ctgatggaat gggtgcagac
     1381 ccgtgggcgc atgcgctcta acaaagcgat gatcggcaga aataatctgc gcggcattgt
     1441 cggtgcactg aagaaaggtg aagcggtatg gtttgctccc gatcaggatt atggtcgtaa
     1501 aggcagctcc ttcgcgccgt tctttgcggt ggaaaatgtc gccacaacca atggtacgta
     1561 tgtcctttca cgtctctctg gcgcagccat gttaaccgta acgatggtaa gaaaagcaga
     1621 ttacagcgga tatcgtttgt acatcacccc agagatggaa ggctacccgg cagatgaaaa
     1681 tcaagccgct gcctatatga acaagattat cgaaaaagag atcatgcgcg caccggagca
     1741 gtacctctgg atccaccgtc gctttaaaac gcgcccggtg ggagaatcgt cgctatacat
     1801 ttaacaaact gctcttactc tttccctcct caaatcagcc ataataacct catggttttt
     1861 atggcttttt tcttaaggtt ttttccttcg ctgaatctct aattattacc gtcagaaatt
     1921 aaactgtcgc cgatatgaga cacctgtaag ttactaaaag taaataagaa aatgcctctt
     1981 gtctcgtttc agttttaggc gtacattagc gccgtctgga gcagtttagc tcagaattct
     2041 ggggtgatgc ccacggtaaa atacaggact tttaaggtcg ttttgcccgg tgtcaactca
     2101 ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa ttacgcggag agatgtaaag
     2161 tgaaatattt ctttatgggc atttctttta tggtcatcgt ttgggccggt acttttgccc
     2221 tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc catctggctc ctgtttgcat
     2281 ttctggcgat ggtgtttatt ccgcgttttc gtgaatatgt ttgctgctgg cgggtagtaa
     2341 accgtcggca cggaacatcg ctttaattcc acgaatcgcc tgacgaatac ggtcgcggtt
     2401 ttcaatcagt gcaaagcgaa catgcgtgtc gccgtagtcg ccaaagccaa tccccggcga
     2461 gacacagacc ttcgcttcgt tcagcagctt cttggcaaat tccagcgagc ccatggcagc
     2521 atacggttcc gggattttcg cccagacata catcgaagcc ttcggcattt cgaccatcca
     2581 gcccgcttca tgcagccctt ttaccagtac atcacggcgg cgtttgtact gttcagcaat
     2641 gtcgcgcacg cactgttgat cgccctccag cgccgcaatc gctgccacct gcaacggcgt
     2701 aaaggtgccg taatcgtgat agcttttaat acgtgccagc gcgctgacca gcgttttgtt
     2761 gccaaccata aagccgatac gccagcccgc catgttgtag cttttcgaca gcgtaaagaa
     2821 ctcgaccgcc acatcgcgtg cacctggcac ctgcatgatt gacggcgctt tccagccatc
     2881 gtagacgata tcggcatagg ccaggtcatg gaccaccagc acatcgtagc gtttcgccag
     2941 cgccacaacc ttttcgaaga actccagctc cacgcattgc gcggttggat tcgacgggaa
     3001 gccgaggatc atcatcttcg gtttcggata actttcgcga atggcgcgtt ccagttcgtt
     3061 gaagaaatcg acaccttcca ccagcggcac tgagcgtacc tgcgccccgg caatcaccgc
     3121 gccgtaaata tgaatcgggt agcttggatt aggcaccagt acagtgtcac catgatccag
     3181 cgtcgccagc atcagatgcg ccagtccctc tttcgaacca atagtgacga tggcttctga
     3241 ttccgggtcg atctcaatgc cgtagcgatc ctgataccag cgggaaatgg cgcgacgtaa
     3301 ccgcggaatg ccgcgtgacg tggagtaacc atgcgtgtcc gggcgctggg ccaccgtaca
     3361 taatttttcg acgatatgcg gcggagtcgc accgtccggg ttacccatac tgaaatcaat
     3421 aatatcttcg ccgcgccgac gcgcagccat tttcagttca gcggtaatgt taaaaacata
     3481 gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg cgagtgtcag ccataatttc
     3541 ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct gccacgatgg tggctgtttt
     3601 gaaaatagcc tgattaattt ctgtctgtcc agcgttttag aacattttta ttaatgacgt
     3661 taaatgagga actttgcttt aaaaacagga aatttatctg cgctaacaag aaaattacca
     3721 cccattttat taacataata atttcaataa atttactaac cagaagtgca ttatcaagag
     3781 atgcattgct aatacaccat ttatctatca atcccctttt aaaattgtgg tttttcctca
     3841 aaaacggcct gctggtcatc agccttcagg ttacctatca tagaggttta atccttattc
     3901 agagtcaccc gtgcacgaaa tattcaacat gctgctggcg gtcttcgatc gggcagcgtt
     3961 aatgcttatc tgcctgttct ttctcatccg tatccgtctg tttcgcgaac tgttgcacaa
     4021 gtcggcgcac tccccaaaag agttgctcgc cgttaccgcc attttctcgc tgttcgccct
     4081 gttcagcacc tggtccggcg ttcctgtaga aggttcgctg gtgaacgtac gtattatcgc
     4141 ggtgatgtca ggcgggattc tgtttggccc gtgggtgggc atcattaccg gcgtgattgc
     4201 gggtattcac cgttatttaa ttgatatcgg tggcgtgacg gcgatcccct gctttatcac
     4261 cagcattctg gcgggttgta tatcgggctg gatcaacctg aaaatcccca aagcacagcg
     4321 ctggcgcgtc ggtattctcg gcggcatgtt gtgtgaaacg ttgaccatga ttctggtgat
     4381 tgtctgggca ccaactaccg cattggggat cgatatcgtc tctaaaatcg ggattccaat
     4441 gatcctcggc agcgtctgta tcggctttat tgtgttgctg gtacaaagtg ttgagggcga
     4501 aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg gatatcgcca acaaaacgct
     4561 gccgctgttt cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga
     4621 tgacattcac gctgacgcgg tggcaatcac caataccgat catgtgctgg cctacgttgg
     4681 cgtgggtgaa cataactatc agaatggcga tgacttcatt agcccgacta cccgccaggc
     4741 gatgaattat ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat
     4801 tcattccatg ctggtgatcc cattgtggga gaaaggcgtt gtgaccggaa cgctgaaaat
     4861 ttactactgc cacgcgcatc agataacctc gtcattacag gaaatggcgg tcggtctgtc
     4921 acaaatcatc tccacgcaac tggaagtttc gcgcgctgag cagctgcgtg aaatggcaaa
     4981 taaggcagag cttcgcgccc tgcaaagcaa aattaatccc catttcctgt ttaacgcttt
     5041 gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc gctcgccagc tgatctttaa
     5101 tctgtcgcgt tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa
     5161 aaaagagctg tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa
     5221 gctgacggtt atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat
     5281 ccagccgttg gtggaaaacg ccattgtcca cggtatccag ccttgcaaag gtaaaggcgt
     5341 tgtcaccatc agcgttgcag agtgcggtaa tcgggtacgc attgcggtgc gggataccgg
     5401 gcacggcatc gatccaaagg tgattgagcg ggtcgaagcg aacgaaatgc cgggcaataa
     5461 aattggcctg ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat
     5521 ccgccgcctg gaaccaggaa cggaaattgc gttttacatt cctaaccaac gcacccctgt
     5581 cgcctcacag gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt
     5641 gaagacgaat tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg
     5701 gagattgtcg gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc
     5761 gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg atggtgtgtt actggcacaa
     5821 aacatcagcc agttcgccca taaaccgttt attgtgttta tcaccgcgtg gaaagaacat
     5881 gcggtagaag cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca
     5941 cgcattaccg ggatgctgca aaaactggaa gcagcctggc agcaacagca gaccagcagt
     6001 acgcctgccg cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga
     6061 atcatcgtca cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt
     6121 gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa
     6181 ctgccgccat cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaatc
     6241 cgcgagatcg aaccgtggtt taataacacc tatattctgc ggctgaaaga tctggatttt
     6301 gaagtgccgg tcagccgcag caaagttaaa gaattccgcc agttaatgca tctttaatcg
     6361 ggaactttca tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc
     6421 agcggcctgg tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc
     6481 tcgttaccgg tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc
     6541 gaatacggca atctgctgga agcaaagcag caacgtatgg ttgaaggaga aatgctgttt
     6601 attccggcgc gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg
     6661 gtgttcgcac cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg
     6721 catccggttc gccagatcca acttcccagc ctgcaacgcg gcgaaggtga agcaatgctt
     6781 actgccctca cccatcttag ccgttcgccg ctggagcaga atatcattca gccgctggtg
     6841 ttaagtttgc tgcatctttg ccgtaacgtg gtgaatatgc cgccgggcaa ttcgcagccg
     6901 cgcggcgatt ttctctatca cagcatttgt aactgggttc aggataatta tgcccagccg
     6961 ctcacccgtg agagcgtggc gcagtttttt aatatcacgc caaatcatct gtcaaaactg
     7021 tttgctcagc atggaacaat gcgttttatc gagtatgttc gctgggtacg aatggcaaaa
     7081 gcgaggatga ttttgcaaaa atatcatctg tcgattcatg aagtggcgca gcgttgcggg
     7141 tttccggata gcgactattt ttgtcgcgtt ttccggcgtc agtttggtct gacgccggga
     7201 gagtacagcg cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc
     7261 aggatgcttt gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt
     7321 ttacgcgcca gctgcgagaa gactttcaca tgattcatcc cttcgttagc gcccagtgtc
     7381 agcatgatca ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcaccagt
     7441 cgggcaatgc tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg
     7501 ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc agacatcctc ttccagctcg
     7561 aacggatgtt cagtgcgccc gttaacgccg aggttaccgc acaggaactg gatcgcctgc
     7621 tctttattgc taaaagcctg atcaacaaag atattctcca gcgccagcag agggcgaacg
     7681 tcttcttccg gcgtaaacgc ggtgagtaac gcttcaatct cctgcgcgct gcggcattca
     7741 catgcctgac gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc
     7801 accgccggaa tgcgcgggct actcatactc agctcgtcca ggcccagccc aagcagtagc
     7861 ggcagataac ggctttcacc gcccagttca ccgcaaatac ctacccattt accccgctga
     7921 tgcgcagtgg taacaatctg ctgcaacatg cgcaggaacg atggcgtaat cgggttatac
     7981 agcggcgata cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg
     8041 gagccgatac tgaagaaatc gacctcatcg cagaagtgat cgatgatgta gcacaccgac
     8101 ggcacttcca ccatgatacc cagcgtaatc gtctctgcat gacgcaggcc atcgcgctta
     8161 agctcaacga tcgctttttg gatctcgccc ttcacccata agatctgatc gaggctgtga
     8221 accattggga tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt
     8281 tgagtgcgga acaggccagc aaattccggg taaattcgta ccgcacgata gccgaggaac
     8341 gggttctctt cctggggaat gttgaggtaa ggaatgcttt tatcgccgcc gatgtccatt
     8401 gtgcggaaga taatcggctt gtcgcccgcg gccagtagca cctgctggta ggcttcaaat
     8461 tgctcctgct cgtccggcgc actgtcgcga tccatataga gcatttcggt acgaaacagc
     8521 ccgacgcctt ccgcgccgtt ggcaaacgcg ccaggcgctt ccagcgcggt gccaatattc
     8581 gccgcgatat caatgcgctt gttatcacgg gaataggcca gctgcgcggc ggcctgcgcc
     8641 tgctgctgct gacgtttatc cgccagcatc tgcgcaacct gataataacc gctcaccgcg
     8701 tcattcgggt taatcgccaa cacgccgcac tgggcgtcaa gcaccgccgg ttgcccggca
     8761 tagcgcgcaa tggcatccag cggcaggccg ctcagtaccg ggatcgccga ggcgcgggcc
     8821 agaatcaggg tatgcgaggt gcgcccggtt ttctccagaa tcatgcccgc aagatttttt
     8881 aaattgaggc tcaaaaactg gctaggggtt aaatcctctg ccaccagaat ggtcggtttt
     8941 tccagcacca gattgttgcg cggttttagt tccggccagg tgatatgcag taactgttcg
     9001 ctgatatcgc gaatgtcgct aacccgctcg cgcagataat cactggcaga agcagaaagt
     9061 ttggcgcaaa cctgctccat attgctgatg atcgccgccc ccagcccctg atgctgttct
     9121 gccatcaggt gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgggcg
     9181 ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt gctggttcag ttgctcggca
     9241 agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gatcgcccgg
     9301 taactgtcga ggctgtcgct ctgtaacagg gtcagcgtac ccacgccgac gccgcttgcc
     9361 agcacattgc cgtacagtaa atcaggattc agacggctta atgaacgcgg cagcggatgc
     9421 gccgtcagtt ctgcctgcgt tggctggacg ctgtcgctgt cgataaagcg cacctggatg
     9481 tactcttcca gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa
     9541 ccgtcattaa atagggtgcc ggtgccaatc agcgccagcg agcttttggc atctgccttt
     9601 gcgttctggc gatggttaat aaaagtgatt tcgctttgcc actggctgca ctgttcttta
     9661 agttcccacg ccggacgagc atgcagaccg tttggcaaag gacagagaaa ttgaatcgtt
     9721 aacataaccg ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc
     9781 tcacgcgtaa gacgttgaat aagggcagat aaaagctgct gcatctgcaa aatgtcacgg
     9841 caatcggcaa tcgatgcggc gcaatgtccg tggcgggtgg caggccccat caccactgtg
     9901 ggtacgccag tgccggttaa gtgcaccgcc ccaccgtccg tgccaccgtt gctgaacata
     9961 tccgcctgca acggcacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt
    10021 ggcggcgcaa tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga
    10081 tggttagccg cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg
    10141 acgtccggcg acaccgcgcg ggtggcagtt tgcccgccgc gtaatcccac ctcttcgctg
    10201 gaacttgcca ccagccacac ctccgcaggc agttcggcgt cgtgcagctc acgtagtaac
    10261 gtcaccagca gatagcaacc gaggcgatca tcaaaggctt tccccatcac ccgctggtga
    10321 gggagaacct gaaaagttgt atcaaaggtg acgcgatcgc ctggacgaat tcccgcctgc
    10381 atcacttcgt catacgagcg cgcaccaatg tccacgcgta tggtgctgac gtcattcccc
    10441 tgccggtcgc cgtcaagcag gcctggaatt ttgcactctt cacgggtagt gatacgcacc
    10501 gactgaagct ggcgggcagc catgcgtaca ttgccaaccg gcaacacatc gatcgcccct
    10561 tcgcgggaga tgctgcgcac cataaatccc acttcgtcca tatgcgcaca gatcatcacc
    10621 ttcggacctg tcgactcatt gagtcggatc agcaccgatc ccagcccatc aaatcgcact
    10681 tctttttgca ggcgatccgc ttcttccagc aggatctgcc gtacttcctg ttccgaggag
    10741 gcgatcgcat ctgcctcgct caacgctttt aataacgata aatccattat gcctctcccg
    10801 tgagcaacac tgttttcggc atggcgtaga gcacttctac gccttgcggg gtgaccagca
    10861 caacatcttc gatgcgcacg cccccttgcc ctggcaaata aatccccggc tccacggtca
    10921 gtaacatgcc tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcctcatgga
    10981 cttcaatacc gatagcatga ccggtgttat gaccgaaata gtcgccataa ccggcttctg
    11041 taatcacccg gcgcgcggcg tcgtcaacct gctggcagcg tacgccgggg cgaatcgcgg
    11101 agattgctgc gagctgtgcc tgcaggacaa tctgatagac gttaaacagc gggtgggatt
    11161 cggcacttac cccttcgcca ttcaccagca aggtgcgcgt catatcagag cagtagccct
    11221 gatacagcgc accgaaatcg agagtgacaa actcgcccgc tgcaacaatc ttgtcgctgg
    11281 ctttgccgtg cggcagcgcc ccacgccagc cgctggcgac aatggtatcg aaagaggctt
    11341 tttctgcgcc ctgctggcgc ataaaccact ccagttcagc ggctatctcg cgctcgctca
    11401 tccccgcctg aataaagcgg cgaatatgct ctgcaccgcg atcggcaatc ccacaggcga
    11461 gacggatttt ctccacctcc tctggcgttt tgagttgccg cagcacatcc ggcgtggcgc
    11521 taaccagttt cgcattgagt tcagactgcc agcggtgcgc ggtttcccaa ctcacctgct
    11581 ggccttcaaa accgagcgtt tgcaactgct catcggcaaa gatttgcctg acgatggtgg
    11641 caagcgtgtg catcgcgtcg agcaaatgca gctggtaacc ttgcgtgcgg gcttctacat
    11701 ccgcgtaata gcgcgagtcc accagaatgt gcgcactttc acggcaaatc accacatagc
    11761 ctgatccggt ggagatcccc agatgcggct gtttgttctg ccgtgaggag agaagcaccg
    11821 catccagttg ttgcgcctta agccagtcgc gcagcgaagc gagtaatgtc atcaggattg
    11881 tccttcttac aggctatcga ttaacaattt gcctttacgg aacatcatca gacgcaggaa
    11941 caccaccatc agcgcagtta tgactgcccc cagcgcgatc cccgccatgt agacgccgag
    12001 gttagtgacc agcggccatg cccagatagc ggactccggg aaccattgca ctgcgcccag
    12061 ccagacagcg gcggttgagc cgacaatcgc gccgaccata taggacggaa tggcggtgat
    12121 ggggctttcc agcgcaaacg gaatcgcacc ctcactgatc cccataaacg ccaggaacat
    12181 ggcggtttta ccctgcggat aaagctgagc actgaacaga cgtttgccgg ttaaacggcg
    12241 gtcgataatg gtcgccagac ccagaccaat cggcggaata acaatagcga tggaacgcgc
    12301 ggtaaccggt aatacgtggt cagtggtaaa gctgaaggca acgaaaccag ctgctttgtt
    12361 gatcgggcca ccaaggtcga ttgctgtcgc cgcggcgatc cccatcgcgt acattagcgc
    12421 acccttctca cctgcggcag tcagcacggt acggataccg ccgttgatcc agccaccaaa
    12481 gggggtgatg acgtagtaca tcgccagcat gacaaaaatg gcggaaagaa tcggtagcaa
    12541 aaatgtggtt ttgaacgcta acaggaaatc cggtaactgg attttctggt tcatccactt
    12601 cacaaggtaa ccagcaacga tagaaataat cagggcacca atgaaggtgg acggcaccgg
    12661 cgaagaggtc gcccattgca tcgtgctggg atcgaagttc agcagttgcg tcggctgggt
    12721 agacatcaac ccaccgataa aaccagccgg gaacgccagt ttgccaccga tagagttcgc
    12781 cacaaatgca gcaaacatcg gaatggcaaa accaaacaac acgccgccga aggactgcga
    12841 caaccaggca aatttcagta aggagaggtc gaaaccggag aatttcccgc tattaagagc
    12901 atccatgatg ccgatatcag caggaatttt aagccagcta taagcaatca gctggctgaa
    12961 agcgaggatc acaccgccca taattaaggt cggcaccatg cgtgaaatcc ctgacatcac
    13021 atgctgcggg agttcacccc agaagctggt tttagaggcc tgcggattct taactgccgc
    13081 tgccgcaccg gatgcgccag gcacaacggt tgcactgcgt tttttaatgg ccataacaat
    13141 tatccctgtc gaatattact gttgttcaga ggcaatcatc tcttcgattt ctttgatgat
    13201 gcccgcagcg tttttaattg cgtcctgcaa agtgatttca taaacgtcgc gtgattcgaa
    13261 acgttcgtta tcttccgggg taaccgccac ggagtggatg atgatggtcg cttcggcgat
    13321 atcctgcgcg gtcaggcgat tctggatgcc gtccgcgccc tgagtttcaa ttttcacttc
    13381 atagcctgct tccaccgccg cttcttccag cgcctgggct gccataaagg tgtgagccag
    13441 acccatcggg caggcacata aagcaatcag tttcttactc atcgttaatt cctcgttcag
    13501 tgatagttga cgagtgaaat tatgaacgtg caaaacaaat cgccgttacc agacaaataa
    13561 tgcatttact ggagaattaa tcccgctgta cggaagtgtg acgcaggtcg gccttgtgag
    13621 gggacacaaa ggaaaattga attaactgta tgatttaaaa gattatcggg agagttacct
    13681 cccgatataa caggaaggat ttagagaatg tgacctaagg tctggcgtaa atgtgcaccg
    13741 gaaccaagaa ggcccggatt gtcatggacg atgagataca ccggaatatc atggacatat
    13801 tctttaaagc gccctttatc ttcaaatgcg gcacggaaac cagaggcttt gaagaactca
    13861 aggaagcgcg gcacgatacc acccgcaata aacacgccgc caaatgtccc gagattgagc
    13921 gccagattgc cgccaaaacg gcccataatg acgcaaaaca gcgacaacgc gcggcggcaa
    13981 tcggtgcagc tgtcagccag cgcgcgttcg gtaatatctt ttggcttgag attttctggc
    14041 aggcggttgt cggctttaac aattgcgcga tacaaattca ccagcccagg gccagaaagc
    14101 acgcgctctg ccgaaacatg accaatttct gcacgcagta tttcgaggat aatgccctct
    14161 tcttcactat tcggcgcaaa atcaacgtga ccgccttcgc ctggcaagct tacccaacgc
    14221 ttatcgacat ggaccagatg cgcaacccct agccccgttc cggcaccgta aaccgcaata
    14281 ggcttacctt ctaccggttc tgcgccacca aactgaatca gatgctcttt tttcagcatc
    14341 gggatcgcca tcgatacagc ggtaaaatcg ttgataattt ccagatggct aaaaccgaga
    14401 ttctttttca tttcggcaat tgagaacgcc caggtatggt tggtcattgc cacccagtca
    14461 ccggtaattg ggcaagcgat ggcaatacag ccgtcttgca cctcgatctt atgttcttca
    14521 agataaacgc gaatgaccgc ttcgaggctg gggtaatcaa gccctgaata ggtcttagcc
    14581 tgcgagattt caccactggc aatatcacac agagcaagac gtgcgttggt gccgcccaca
    14641 tcaccgacta atgcatactt tgtcattctt caactgctcc gctaaagtca aaataattct
    14701 ttctcacact gtaaatacct gggggcataa caacaacgcc ggaaaggcag gctccctgta
    14761 aatatcgatc tgggtcacac aattacttta tcgtttcagc accaattgca gcgatgcctt
    14821 tttgcaagct gggcaaacta agtatctgac cccgcataag gaatagaaca tgctccatcc
    14881 gcgagccaga accatgttgt tattatcgct ccccgccgtg gcaattggga ttgcgtccag
    14941 tcttattctg attgtggtga tgaaaatcgc ctcggtatta cagaatttgc tctggcaacg
    15001 actgccggga actctgggga tagcccagga ttcacctctc tggatcatcg gtgtattaac
    15061 gctaacgggt attgcagtgg ggttggttat ccgtttcagc cagggtcatg ccggaccaga
    15121 ccccgcctgt gaaccgctga tcggcgcacc agttccgccc tctgcgctac caggacttat
    15181 cgtagcatta attcttggtc ttgctggtgg cgtcagcctg gggccagaac atccgatcat
    15241 gaccgtcaat atcgcccttg cggttgccat tggcgctcgt ctgttaccgc gcgtcaaccg
    15301 aatggagtgg actattttag cctctgccgg aaccatcggc gcgctgtttg gcacacctgt
    15361 tgcggcggcg ttgatatttt cgcaaacctt aaatggcagt agtgaagttc cgctatggga
    15421 tcgtcttttt gcgccgttaa tggcggcagc agctggtgca ctaactaccg gattattttt
    15481 ccatcctcat ttttcactgc ccattgcgca ttacggacag atgcaaatga ccgatattct
    15541 cagtggtgca attgtcgcgg cgattgccat cgcagcaggg atggtcgccg tatggtgctt
    15601 accacgattg cacgcgatga tgcatcaaat gaaaaatccg gtgctcgtgc tgggtattgg
    15661 cggatttatt ctcggtattc tgggggttat gggtggacca gtttcgctgt ttaaagggct
    15721 ggatgagatg cagcagatgg tggcaaatca ggctttcagc accagcgatt actttttgct
    15781 ggcggtgatt aaacttgccg ccctggtcgt tgctgccgcc agtggctttc gcggtgggcg
    15841 aatcttcccg gcagtgtttg tcggcgtggc attagggttg atgctgcatg agcacgtccc
    15901 cgccgttcca gcggcaataa ccgtttcctg cgctattctc ggcatcgtgc tggtggtaac
    15961 acgcgatggc tggttaagtc tttttatggc ggcagtcgtt gtacccaata ccacattgct
    16021 accgctgctc tgtatcgtca tgcttccggc atggctgtta ttagcaggta agccgatgat
    16081 gatggtcaat cgtccgaagc aacagccccc ccacgataac gtttagcaaa aaatgctctc
    16141 ctttatgatt taagagttat ggcgatgata cggggcttta cctccccgta atattgcgtt
    16201 aacaggccgc tgacggcttc gatcgtgaag gagaataaca atgttcaggt cactgtttct
    16261 ggcggccgcc ctgatggcat ttaccccgct tgcggcaaac gcaggtgaaa tcaccctact
    16321 gccatcaatc aaattacaaa ttggcgatcg cgatcattac ggtaattact gggacggtgg
    16381 tcactggcgc gaccgtgact actggcatcg caattatgaa tggcgtaaaa accgctggtg
    16441 gcgtcatgat aatggctacc accgtggctg ggataagcgt aaagcgtatg agcgtggcta
    16501 tcgtgaaggc tggcgcgatc gtgacgatca tcgcggaaaa ggccgcggac atggacaccg
    16561 ccattaaaca cattcgccaa tggagcacaa tgcctgatgc gacgctagtg cgtcttatca
    16621 ggcctacaaa ccggctcttt aacgcgtagg gcggataagg cgtttacgcc gcatccgcca
    16681 atagtgccag atgcgacgct cattcaacta caatcccagc gccgtcccca ccaacaacca
    16741 gatattcagc gccacgacca gcactacaat cacccagcct gtctgtttta cgcgtttgct
    16801 gttcaccaga tcgcccatca acttgctgtc actggtgaaa atcagcagtg gaaccagcgc
    16861 cagagcgata ccaaaactta acagcacctg actcataacc agaatccgtg tcggatctaa
    16921 tcccatcaga atgacaataa atgacggcaa catggtaacg gtgcgacgca cccacagcgg
    16981 gatatggaag cgaatgaagc cctgcatcac cacctgcccc gccagtgtcc ccaccaccgt
    17041 tgatgacagt ccggcagcaa ccaggcttaa tccaaagacc gttgccgcag catggcttaa
    17101 caacggttgc agcgtcaggt aagcctcatc aagatcggca acgccagtat gaccggaaaa
    17161 gtggaacgcc gccgcagccg tagccatcat cgccagattg acaaaaccgg caatagtcat
    17221 agcgatagcc acatcccatt tggtggcaga ataacgttgt tgacgcgaac caccatgtaa
    17281 atgctgagtc agcgaagagt gtaaataaat cacgtgtggc ataatcgtcg cccccaacac
    17341 acctgccgcc aggaagactg cctccgaggt gggtaaactc gggattacca ttcctttacc
    17401 cagttgcgcc aggttaggct gggagaaaat caactcgaca atgtaagccg cagcaacaaa
    17461 cagcagtaac ccgccaatca ctttctccag cggtttttgc cctcgacgtt gcagcattaa
    17521 aatcaggaaa gtcgcgatcc ccgtcagcac cgcgccctgc aacagcgaaa cacccagaat
    17581 gagtttaaaa ccgatcgccg caccaataaa ttccgccagg tcggttgcca tcgcaatgat
    17641 ttctgcctga acccaataga accacactac gggacgcgga tagtgatcgc gaatctgctc
    17701 cgccaggttt ttaccggtgg caatccccag tttggcagac agtatctgaa tcagcatcgc
    17761 catcaggttg gcccaaacga caacccacag tagctgatag ccgaagctgg cacccgcctg
    17821 aatattggtc gcaaagttac caggatcgat ataaccaatc gccgcaatga acgcaggtcc
    17881 cattaatgcg agcctcatct tgcgcgccgc ccgtccgctg ctactctcaa cgcgatagtt
    17941 agtcatcttg tgcctctaaa acatagcctt tgctatgttt catgctatgt caaacgagaa
    18001 tgattatcaa attcatttaa gtggattgtg atgatttctt tgatagacca gaattatgac
    18061 gacgaaaaga ttgatgactt gaatgttgtg attatgttta atgttagcac atttacataa
    18121 ctttcagctt ccatacacaa catagcagaa atgtatgaca gctcactatt tttgaagctt
    18181 gtcacaggac gtcattatag tgtgtgtcag atctcgtttt ccttaaccat gttacataga
    18241 atgtgcacgg aaatttaacc tgcctcatat ttggagcaaa tatggaccgc gtccttcatt
    18301 ttgtactggc acttgccgtt gttgcgattc tcgcactgct ggtaagcagc gaccgcaaaa
    18361 aaattcgtat ccgttatgtt attcaactgc ttgttatcga agtgttactg gcgtggttct
    18421 tcctgaactc cgacgttggt ttgggcttcg tgaaaggctt ctccgaaatg tttgagaaac
    18481 tgctcggatt tgccaacgaa gggactaact tcgtctttgg tagcatgaat gatcaaggcc
    18541 tggcattctt cttcctgaaa gtgctgtgcc caatcgtctt tatctctgcg ctgatcggta
    18601 ttctccagca cattcgcgtg ttgccggtga ttatccgcgc aattggtttc ctgctctcca
    18661 aagtcaacgg tatgggcaaa ctggaatcct ttaacgccgt cagctccctg attctgggtc
    18721 agtctgaaaa ctttattgcc tataaagata tccttggcaa aatctcccgt aaccgtatgt
    18781 actccatggc agcaacggcg atgtccaccg tgtcgatgtc catcgttggt gcatacatga
    18841 ccatgctgga accgaaatac gtcgttgcag cgctggtact gaacatgttc agcaccttta
    18901 tcgtgctgtc gctgatcaac ccttaccgtg ttgatgccag tgaagaaaac attcagatgt
    18961 ccaacctgca cgaaggtcag agcttcttcg aaatgctggg tgaatacatt ctggcaggtt
    19021 tcaaagttgc cattatcgtt gccgcaatgc tgatcggctt tatcgccctg atcgccgcgc
    19081 tgaacgcact gtttgctacc gtgactggct ggtttggcta cagcatctcc ttccagggca
    19141 tcctgggcta catcttctat ccgattgcat gggtgatggg tgttccttcc agtgaagcac
    19201 tgcaagtggg cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg
    19261 atctgcagaa aattgcttcc acgctctctc cgcgtgctga aggcatcatc tctgtgttcc
    19321 tggtttcctt cgctaacttc tcatcaatcg ggattatcgc aggtgcagtt aaaggcctga
    19381 atgaagagca aggtaacgtg gtttctcgct tcggtctgaa actggtttac ggctctaccc
    19441 tggtgagtgt gctgtctgcg tcaatcgcag cactggtgct gtaagaccat acataaaaaa
    19501 gccggggata attccccggt tttttacgcc tgttaatcag ctaatggctg cgggcgacca
    19561 atcaaatacc cttgcagata ttgcaccccg agcttatgca atagcgcctg ctgctgttgc
    19621 gtctcgacaa actccgcgac cacactcaat gacttcgctt tcgccagatc ggtaattgat
    19681 ctcacaatca tcgcatccag cgtgttcgtg acgatatctt tcacaaagac gccatcaatt
    19741 ttgatgatat cagcctgcaa acgctttaac cgttcgtagt tggcatatcc ggtgccaaag
    19801 tcatcaatcg caatccggaa accaaactta tgcagctgct cgatgttgta catgctgctt
    19861 tctgcgttag aaaacgcctg ctcctcggtg atctcaagaa tgaccgcctg cggggagatg
    19921 tgataacgct taaacagacg aataatccgc ccggcaatat tcttttgcag cagcgtgagc
    19981 ggcattaaat tgactgaaaa gcgcgggcct tttttgtcgc aaggatgtgt tgccagccac
    20041 ttcaacaggg attccagcac ttgcaaatca aaacgcgcgc taaggttaaa ctgagcaata
    20101 aggggcagaa acttatccgg ggtcataatg ccgccgtcat atttcagtcg cgcgaggatc
    20161 tcatcataac cttcaccctc tttgttgcga attggctggg cgtagagcag caaatcaccc
    20221 tgatctaacg ccgtacgaat ggtattcagc aatagcacct gtttagtggt ctgcccggaa
    20281 accatctcct ctctgctatc cagcgccagc acatggtgat gtgcgcagga ttgctccgcc
    20341 agccagctta actgccccaa caagggttgc agggtttcct gatttccatc aaaacgcccc
    20401 caggcagcac catagcccat atccagcccg gtattgttcc agtgaatttg ccgactattc
    20461 aggatgttaa ccatatgctg gagtcgccct tccgtttccg gtccactcag cactaacagc
    20521 agttcactac ccggcaattg atacaacttt tcgttttcct gcatcaacgg cagcagcgta
    20581 cggtaaattg atcggataca gtgaacgcgc atcattaagc cgtaatgacg actcataaac
    20641 tcaagattat caatgcgcag gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgt
    20701 tctaacgcac gaaagttggg taggagcgtt aacggatcgg tcagcgcctg caaatgccag
    20761 cggcgattaa gccattcact gcgatgataa atgcgcacca tatagagcag gcaaacgcta
    20821 aaggaaatca gcaccgcgag aataaatgcc agcgaatatt cggttagtac cccttgcaaa
    20881 aagttctggt tgtaattcag caggcaaagc gttgaaacag cccaggtgag atttaaaaac
    20941 ggatagcgaa gcttaccgac accgagggta aagatgataa agaaaacagg taccagataa
    21001 ccagcaataa agtcattttc atacggtgtg cacatcagaa gtagcagcac gctaagagct
    21061 gccagccagc ttaaggtaaa tgcgcgtttc tctttgctca acgacggagc gatatcccta
    21121 cgccacaata tctgtgcaaa gtggggactt acaatcatgc gggcgagata gtaaaaaagc
    21181 atgttgtaaa tcagcactgc ggtgaaaagg cttagcaaat caacgaccgt gaaaatggca
    21241 tccgcatcgc cgaaaaaggt agatatcttt agcggaaaat caaagaaatt tcccacaaga
    21301 tacatgctgc atttgatgcc aatcggcgcc accaaaccaa gccagaaaag acgttgccag
    21361 acatatcggc tggtcaatcc ataacgccaa cgcgtcccca gctgccagcg taatattgcg
    21421 caggcacaca acaccacaaa cgtctggctg ccgagcatga ccgcggtctg taaaaaagag
    21481 agatgaaagt tccacagatt ggtgcaaaac atcccgcata aaacggggac gacaccgcgc
    21541 cagccaaaga taaacaagat tgacagcatt acgcacagtg gcatccacgc cagaaagata
    21601 taactggagt ggataatggc aagtggcgaa ataaaacggg atagctgaat aagtaccacg
    21661 gtgagcgtaa acgctagtgt gaatatcttg atatttttta tcaggttatg ctccacaaac
    21721 atgagtacac tactctcact gatcggggtt ttcaggcaca caaataataa gtttattcgc
    21781 atcgcgaagc aaacaggggc cagcctgttg ccgatttttc atccaatgat gctttcacca
    21841 ggcggcgcta acccggacgt agactgtatt acaaaagcgg caaaaagcag agacaaaaaa
    21901 cccccgcttt acagcgaggg tttgaaattt ggtggagcta agcgggatcg aaccgctgac
    21961 ctcttgcatg ccatgcaagc gctctcccag ctgagctata gccccacgat gcgtttacgt
    22021 accaaatttg ctgggtgcaa aatttggtgg agctaagcgg gatcgaaccg ctgacctctt
    22081 gcatgccatg caagcgctct cccagctgag ctatagcccc atcacgtaaa gcttgtcgag
    22141 ttgacgggcg gcatcatatg aattccgccc gaatgtgtca acggcaaatt gccacgtgta
    22201 gtttcaatcg ctgaaaaatc aggcaaatga acaattttgg aaaaccgctc gcattcagta
    22261 gttattcatg tcacggtttc ctgtaaagtg gtgttataaa atgaactact aatagaccca
    22321 catacattca gggaattgtt atgttcaagg agaggatgac gccagatgaa cttgccagac
    22381 tgaccggcta tagccgccag accattaata aatgggtacg caaggaaggc tggacaacgt
    22441 caccaaaacc aggcgtccag ggtggcaaag ctcgactggt tcacgtcaat gaacaggttc
    22501 gtgaatatat tcgcaatgct gaacgtccag aaggtcaggg agaagcgcca gccctttctg
    22561 gtgatgcgcc gcttgaagtg ttgctggtga cactggcgaa agagatgacg ccagttgaac
    22621 aaaaacagtt tacatccttg cttctgcggg aagggattat cggattgtta caacgcttag
    22681 ggattcgcga tagcaaataa tatgaaaaga ttacgcaata aaatgaccac cgaagaactg
    22741 gctgaatgcc tcggtgtggc taaacaaact gttaaccgtt ggatcagaga aaaaggctgg
    22801 aaaacggaaa aatttcccgg cgtgaaaggc ggtcgtgcca gacttattct ggtcgatacg
    22861 caagtttgcg agtttattca gaacacgcca gccttccata acacaccgat gcttctggaa
    22921 gccgaagaac cccttgccga atacgcaccg ggtattcgca ctcctgctta tcggcaaatc
    22981 atcagcgcaa tagacaacat gactcacagt gagcaggaaa aagttgcgca atttttgtca
    23041 cgcgaaggaa ttcgtaattt ccttgctcgt ctcgacatag atgaatcaga ataaataaaa
    23101 aacggcaggg gtatctctcc tgccgtttat cttttatgcg ctaattattg ctgattttcg
    23161 cgttcagcaa taaaagccag cgctttgttg atacgctcga tactgcgggt cttaccgatt
    23221 gcgtgaacgg taacatccag cgccggagac tgccccgcac cggttacggc gacacgcagc
    23281 ggcataccaa ctttacccat acccacttcc agctcatccg ccgtcgcctg aatagcgtga
    23341 tgaacgtttt cagcggtcca gtcagtaatc gcagtcagtt tgtcacgaac cacttccagc
    23401 ggctgacgcg ctaccggacg cagatgtttt ttcgcggcgt cggcatcgaa ctcagcaaaa
    23461 tcttcgtaga aataacggca gctctgtgcc atctctttca gcgtcttgca gcgttcgccc
    23521 agcagtttca ccagatcagc cagctgcggg ccgttacggg tatcgatatt ttcctgctca
    23581 atgtgccatt gtaagtgagt agcaacatac tccggcggca gcgcgttaat gtagtgatgg
    23641 ttcagccaca gcagcttgtc ggtgttgaac gcactggcag atttgctgac ggcattcaga
    23701 gtgaagtatt tgatcatctc ttcacgagtg aagatttcct gatcgccgtg ggaccagccc
    23761 agacgcacca gatagttcag cagtgcttct ggcaaataac cgtcatcacg atactgcatt
    23821 acgctgaccg ccccgtgacg tttggacagt tttttaccgt catcgccgtt gatcatagaa
    23881 acgtgcgcgt aaaccggtac cggcgctttc agggctttaa gaatgttgat ctggcgcggc
    23941 gtgttgttga tatggtcttc gccacggata acatgggtga tttccatatc ccagtcatca
    24001 acaaccacac agaagttata ggttggggaa ccatcggtac ggcgaatgat cagatcatcc
    24061 agctcctggt tgctgaactc aatcgggcca cggatctgat cgtcaaaaac aacagaacct
    24121 tcctgcgggt tagcaaaacg tacaacgcac ggttcatcat cagcatgatg ctcatggctg
    24181 tggcggcagc gaccgtcata acgcggcttc tcacctttcg ccatttgctc ttcgcgcagc
    24241 gcttccaggc gctctttaga acaatagcat ttataagctg tgccctcttc cagcatctga
    24301 tcgatcaccg cgttgtagcg atcaaaacgt ttggtctggt agtacggacc ttcatcccac
    24361 tccaggctca gccagttcat gccatccata atggcttcga tagcttccgg cgtggaacgc
    24421 tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc cgtggttacg tgcaaaaagc
    24481 caggagtaaa gagcagtacg cgcgccgcca acgtgcagat agcctgttgg gcttggcgcg
    24541 aagcgagttt tgattttcat gaaatggcct tacgtttaga aagatgccga caaccggcaa
    24601 atcctggaaa aattgagtgg gcgatattct atcactcccg cctgattcct caatgtagtt
    24661 cgggttgcaa ccttacgcat tattgttttc gttgacaaat tgcgcaacac ggtctgtttt
    24721 gcgatcatct tgcttaaatt tacgacgaac gaacaatttc tttagaaaat gcgttgactc
    24781 attttgaact ctccctataa tgcgactcca cacagcgggg gtgattagct cagctgggag
    24841 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accaactact
    24901 ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg gtgattagct cagctgggag
    24961 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accactttct
    25021 cgccagctag atttcttgta acaatgtgaa gtaccgaagt gggtgattag ctcagctggg
    25081 agagcacctc ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttc
    25141 gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat
    25201 cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct atctgcaatt
    25261 tgcgaaattg cctgatgcgc ttcgcttatc agaccaacta tgtccggcag ttcccgtgcc
    25321 tctcatctac tgcgccaacg ccgccagcag tttaaccagc gcgggcgtca cctgctgcgt
    25381 ttcataaaca atatataagt ctgccgggat gcgttctttg agcggacgga atatgacacc
    25441 gggccagttc atctgcgcgt agctgtccgc tatcaatgtg ataccaatac ccatactgac
    25501 catggcgagt accgtttgcg gttcattaac ttcgcgaata acaaccggtg aaaatcccac
    25561 ctgctggcaa actcgctgca aaaaatccca gtcagtgtac acgggcggca ttgtaacgaa
    25621 atactcgtca cgtagcgctt ccagcgggac ggtggaaaat gatgagagat gatgctcttc
    25681 aggcatcgcc accagaaacg ccgattcatg caaccgtaag ctggtaaaac cagtcggtgg
    25741 ttctgtcgcc attcgccaga tcccggcatc aagttcgcgg cgttccagca aggccatttg
    25801 catcgcaggc atcttttcgc gaaacagaac ttcaacgtta ggattttctc taaggaatcg
    25861 ccgcataacc gggcgcatcc gtccccacat tgccgttccc acaaccccga gttcaatccg
    25921 ccctgcttct ccccgaccta tttgttcaac ccgagccaat acattatttg cattcaccag
    25981 caatcgacgc gattcttcca tcaagatttt gcccgcgtgt gtcagtacga cgctgcgcga
    26041 atggcgaata aaaagctgcg tgccgagttg attttccagc tctttaatat gaatgctgag
    26101 cggaggctga gacatattta aacgcgctgc tgcgcgacca aaatgcaact cttccgctac
    26161 ggcaagaaaa taacggagca acttaagatc tgttctgtat acgcgttcca taattaagtg
    26221 ctccatataa taaatacatc gtattaaatt atcatggcat aaagtatttc accaccagat
    26281 atccgacata cataaacaca atggataata tacccggtaa tacatttggt acaggaatag
    26341 gcaagcgcac gattgtaaac aaagcaccaa tgccaaaacc aacgccagaa gaaaataaaa
    26401 tatctttcac taattaacct ttatcataaa agcagcccca aggaacagag ctgcgaatcc
    26461 ttttaatgtg taactgaacg atgcgttatc ttttcagggt catggttata tttaaaccca
    26521 aagaaaaata tcactgcgag gaaaagagca taccctgcaa acatcagcca aatggtatgc
    26581 caatctttta cgccatccac cgagaagtaa tccactgcca taccgctcag aatcgagcca
    26641 acccatgcgc cgacaccatt taccatggtc ataaaaaggc cctgggcgct ggcacgaatg
    26701 ctggaatcaa cttcctgttc gacaaatacc gaaccagaaa tattgaagaa atcgaatgca
    26761 cagccataaa caatcatcga cagcagcagc aaaataaacc cggttggtga tgggtcgcca
    26821 taggcaaaga agccaaagcg cagcgtccac gccaccatac tcatcagcat gacggtttta
    26881 ataccgaaac gcttcaaaaa gaacggtatc gtcagaatga aaccgacttc agccatctgt
    26941 gaaactgaca gtaaaatgga cggatatttc accacgaagc tatcagcaaa ctcaggatta
    27001 cgggcgaaat catgcaggaa cgggttgcca aaaacgttgg taatttgcag caccgcgccc
    27061 agcatcatgg cgaagaggaa aaagatagcc atgcgtggat ttttaaacag cacgaaggca
    27121 tccagaccca gcttgctggc aagcgatgta gtcgcttttt tctccgcaac cggaatcttc
    27181 ggcaaagtca gcgcataagc cgacagcagc aatgacgcac cggacgcgat atacagctgc
    27241 agactactca attccagatg cagcaggctt actgcccaca tggcgacaat gaaccccacc
    27301 gtaccaaaaa cgcgaatggg cgggaaagcg gtcaccgggt caagccctgc ctgggcaaga
    27361 caggaataag agacgctgtt cgataacgca atagtcggca taaacgccat cgcattgact
    27421 aacatcaccc aaaacatcat atccggatca gttacggatg ccgcataaaa aagtacgccc
    27481 gcacacacca ggtgacacag catgtatgca cgttctgcac gcagccattt gtctgcgatg
    27541 atccccatta tacccggcat aataatcgcg gcgatccctt tggaactgta aaccatgcca
    27601 acattagcgc cggtgaaatg aagggtatta atcatgtaag aaccgagggt aaccagccag
    27661 ctcccccaga taaaatattg caaaaaggac attaccttta agcgcaaagc gatgctcata
    27721 atcgtttcct tgtcgcatca tatggcctca ctcagtaagg ccgtttattg ttgttttact
    27781 caggcaattt tgcgcagaaa gccgcaaata agattaataa agttttgctt tgagagttcc
    27841 gctgctgcca gcgtttgggc atgagaaagc ttcacgtcac tcagaccttc cgccatgttg
    27901 gtaatcgcag agaccgcaac gactttaagt tcgcaatggc gagctgaaat aacctcaggc
    27961 accacagaca taccaacaac atccccacca ataatttgca tcatacgaat ttccgccgca
    28021 gtctcgaaat tcggccccgg atacgagacg aacacgccct ccgtcagagg gaacccctct
    28081 tctttcgcca ctttttgtaa cagtgcgcgg tattccgcat cgtaggcatt cgccagcgag
    28141 aagaagcgct caccaaaacg ttcatcatta agacccacca tcggcgttcc cggcatggtg
    28201 ttgatgtgat ctttcaatgc gaccagactg ccggccccca cttcaggacg cagtgagcca
    28261 gccgcattgg tgcagaacag caactcgcag cccagcaact taaaggtacg gattgcatcc
    28321 gtcatgatgg tcatcccacg accttcgtag aaatgtccgc gacctttcat gcacgccact
    28381 ggcaccccct ggagataccc cagcaccagc tcacccgcat gaccgtgtac ggtacttacc
    28441 gggaacccag gcagcttttc gtaggaaatt gcgaccgcgt tctcaatctg atcggccagc
    28501 gcgcccagcc cggaacctaa aataaaggcc actcgtggcg tgaaatcagg tttataagtc
    28561 ttgataatat ctacgcaata cagtgggtta tgagaaaact gagcctgaga catatatatc
    28621 ctttttttgt agggtggaat ctaacgcctt tttataacga gattgcccta cctcatacca
    28681 atacactttt ttcatgtatc cgatagttat tcgatatcga tatatttata ttttttcact
    28741 attaataggc tgaaaaaact gtattggtac tctcctttcc cttgcattaa caatatgccg
    28801 cttttaatca accttttatt aagcaatcaa ctaataacga tcggcatagg gaattatgct
    28861 atgaaaaaac atctttttac tctgacactc tcttctgtat tggcgatacc agcagtatcc
    28921 catgcagaat ttaaaggcgg ttttgctgat ataggtattc attatcttga ctggactagc
    28981 agaaccactg agaaatcgtc gaccaaatca cacaaagatg attttggata tctggaactg
    29041 gaaggcggcg ctaactttag ttggggagaa atgtacgggt tcttcgactg ggaaaacttt
    29101 tataatgatc gtcatgataa accgggtagc gagcagcgtt atacctttaa aaataccaac
    29161 cgtatttacc tgggtgacac cggatttaat ctctacttgc atgcgtatgg cacctacggt
    29221 tctgcgaatc gcgtgaattt ccatgacgat atgttccttt acggtatcgg ctacaatttc
    29281 accggtagcg gttggtggtt caaaccgttc tttgcaaaac gctatacaga ccaaacctat
    29341 tataccggcg ataacggtta tgtcgccggt tgggttgcag gttacagctt tatgctgggc
    29401 agtgagaaat tcaccctcac caactggaac gagtacgagt ttgatcgtga tgccacctac
    29461 gcggcaggta atggcggcaa agaaggtctg aacggtgccg tcgcactttg gtggaatgca
    29521 acttcacaca tcaccacagg aattcagtat cgttatgcgg atgacaaact gggcgaagat
    29581 ttttaccagg atgcaataat ctactccatc aaatttaatt tctgattgat tactgccggg
    29641 gctcccggca gtatgaatag ctctatatct gatacagcgg atccccttcc cgcccagcca
    29701 ttagcgcacg gccctggcct cgtaccactt cccataacgc ttgtgcggcg gtggaaagtg
    29761 aacggttctt cctgcgcacc agcatcaact ggcgttccac caccggcgta atgcgcttta
    29821 ccaccagcgg gctaccttca ggtaacggca gcgccagtgc gggaagaata ctaatgccga
    29881 tgcctgcggc taccatcggg aacagcgtcg ccggatgacc aatctcctgc acaatattcg
    29941 cctgaatacc gttgcgcgcc agtgctgcat caatcagcgg tcggctgcct gacgcgtaat
    30001 cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct tccgcggcca
    30061 aagcgctatc gcgatggcaa agcaagaaga aaggctcgga aagaatcgct tcacattgca
    30121 gatcgctcac tgggcctgga tcgatgacga tgccaaaatc gacatctccc tggcgaatac
    30181 tttccatcac ccactgctgc ggacgatcgt gcaggataaa ctgaatatct ggatagcggc
    30241 gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcga
    30301 cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa
    30361 gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca
    30421 cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac
    30481 tgtgactcac tgccgactgg ctcaggccga tacgctctcc tgcacgacta aaacttttct
    30541 cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc ttttttcttc
    30601 atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat
    30661 cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg
    30721 atcacggtgg tgttgctggc ctctttcttt ccggccagag gcgatttcgt ccccttcttt
    30781 gaaaatctga ccactgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg
    30841 cgtgaggcga ttattgctgg cggtgggcac tggcgactgc atttgtgggt aatgtgcagc
    30901 accttcgtat tgtttccgat tctgggcgtg ctgtttgcct ggtggaaacc ggtaaatgtc
    30961 gacccgatgc tctactccgg ttttctctac ttgtgcattc tccccgctac cgtgcagtct
    31021 gcaatcgcct tcacgtcaat ggcgggcggt aacgttgcgg cggcggtttg ttctgcgtca
    31081 gcatccagcc tgcttgggat tttcctttca ccattgctgg ttggtctggt gatgaatgtt
    31141 cacggtgcgg gaggcagcct tgagcaggtc ggtaaaatta tgctgcaatt gctactgccg
    31201 tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa
    31261 tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacggc attcagcgaa
    31321 gccgtcgtta acggtatctg gcacaaagtt ggctggggat cattgctgtt tatcgtggtg
    31381 gtcagttgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg
    31441 ggcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca
    31501 aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg
    31561 cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac
    31621 aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa agcttaaagc
    31681 ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt
    31741 tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga
    31801 tactcctccg gcaggtcgat taagcgacgc cctttgtact tgccaaacgg cattatcgta
    31861 ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc
    31921 cgtttcgtcg atgacttcaa tacccagttc ctgcgccttc gccagtttag atcctgcagc
    31981 ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag
    32041 ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac
    32101 cacggtttta ccagcaaacg ggctgtcaat ctcttccgcg ttaatcacca ccggcgcagg
    32161 ccagtgaacg ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa
    32221 gaagttgtga acgtgggagg caacgacaat gccaacatca ggcacctttt gcagctcttc
    32281 aatcgaagcg gcttccagcg cttccagcgt gccgaaatat gccgccagac ctgctgcggt
    32341 ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt
    32401 cgctttttcc agcgcgttaa ccacgttttg tgccgatttc ggccccatac gctccagtcc
    32461 ggtcagtttg actgcggtga gtttgaacag atccgccgga gtgtggacat attctttttc
    32521 aaccagttga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac
    32581 aaagtgtttc agagactctt tacgctgcgc accgcaaatc aggccaccgg tacagcgggc
    32641 aaccgcttca ccttccacac gctcgacgtc agaaccacat accggacaat gcgtcgggaa
    32701 tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca cctgcgggat
    32761 cacgtcgcca gcgcggcgaa taaccacttt atcgccaatg cgtaaaccaa gacgttcgat
    32821 ttcgtcagca ttatgcagtg tagcgttact caccaacacg ccagcaacat ggacaggttc
    32881 cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac
    32941 aaaggtcatc tgttcctgcg ccgggaattt aaacgctacc gcccagcgcg gggcacgcgc
    33001 gacaaagcca agctgctcct gctgtgccag tgagttgacc ttaatcacca cgccgtcgat
    33061 atcaaagccc agcgtcggac ggtctttttc tacttcgtga taaaacgcca gcacttcttc
    33121 cgccgattca caaagtgtta cccgatcact gacaggtaat ccccacgctt taaattgcat
    33181 taagcgtccc aaatgggtat ccggcaattc accgccttcc agaacaccaa cgccatagca
    33241 gaagaaagtg agcggccgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc
    33301 tgccgcatta cgtgggttag caaacacttt cccgcccgtg cgtcgcgcat cttcgttaat
    33361 cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat
    33421 attctctccg tgtagcttca gcggaatggc gcgaatagta cgcacattag acgtgatatc
    33481 ttccccggtg gtgccatcgc cacgggtggc ggcactgact aaaacaccat tttcatacag
    33541 aatactgacg gcaagaccat ccagcttcag ctcacagcac caggtgactt tctcgttgct
    33601 tttcagacgg tcctgcacac gtttgttgaa agcaagaaag ctttcttcat caaaaacgtt
    33661 atccagcgac agcattggca cttcatggcg gatctggctg aaagccgcca gcggcgcagc
    33721 acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc
    33781 gcgcaattcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac
    33841 atgataaaga tattcatgat ggcgaagcgt cgttcgcagt tctgtcagtt gttgttcgat
    33901 tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag
    33961 ttaatttgcc ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg atgtcctggt
    34021 actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagaaca acaccgccca
    34081 cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac
    34141 cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag
    34201 gatcaaaggt tcccggtttc accatattcg ccaggctgaa taacgccggg ccgctgccat
    34261 ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa
    34321 tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga
    34381 taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct
    34441 caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg
    34501 ctgaatgcac tggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct
    34561 gttcaggctg cggctgatag gcaggctgct gcacgggttg ctgcaccagc tgcgctggac
    34621 gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt tgacgcggct
    34681 gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca gcctcatgct
    34741 cctgggcgtt agccggggca tgattcacgc ggtgaactcg aacctcacca acgccctcat
    34801 catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg
    34861 gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca tgtaccagta
    34921 aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta
    34981 tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact aagagtattt
    35041 gtcgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc
    35101 gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa
    35161 atacgcagaa ttttcctggg attggtcaaa aggagctcat cctgattatg gtttcatcat
    35221 tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc
    35281 aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg
    35341 gcgcattctg gtggctcttt acgcaactcg atgtctggat cccgactctc atgagttatg
    35401 ttccggactg gttacaatgg ctgagctatc tgttgtggcc cctggctgtc atctctgtgc
    35461 tgttagtatt tggttatttc ttttcaacga ttgctaactg gattgccgct ccgttcaacg
    35521 gtttattggc tgaacaactg gaagcaagac tgactggcgc tacaccgcca gataccggaa
    35581 ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgcct
    35641 ggtatctgcc gcgcgcaatt gtattgctaa ttctttactt catccctggt attgggcaaa
    35701 ccgtcgcgcc agtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg
    35761 attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac
    35821 gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg atcccgttgc
    35881 ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct
    35941 atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt
    36001 gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc
    36061 tgcatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt
    36121 tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga agataactcg
    36181 ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg
    36241 gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg
    36301 atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccaacc
    36361 agcggtaata ccgggattgc actggcctat gtcgctgccg ctcgcggtta caaactcacc
    36421 ctgactatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca
    36481 aacctggtgc tgaccgaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa
    36541 attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac
    36601 cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga cggtcaggtt
    36661 gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt
    36721 aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca
    36781 gttatcgccc aggcgctggc aggtgaagag attaaacctg gtccgcataa aattcagggt
    36841 attggcgctg gttttatccc ggctaacctt gatctcaagc tggtcgacaa agtcattggc
    36901 atcaccaatg aagaagccat ttctaccgca cgtcgtctga tggaagaaga aggcattctt
    36961 gctggtatct cttctggagc agctgttgct gcggcgttga aactacaaga agatgaaagc
    37021 tttaccaata agaatattgt ggttattctg ccgtcatcgg gtgagcgtta tttaagcact
    37081 gcattgtttg ccgatctttt cactgagaaa gaactgcaac agtaatgcca gcttgtaaaa
    37141 aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc
    37201 tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg
    37261 cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc
    37321 ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc
    37381 aatggactgt attacgctct tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca
    37441 ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg
    37501 gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg cacacccgcc
    37561 ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca
    37621 acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag
    37681 gtaccgttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt gaacatctgg
    37741 ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta
    37801 aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct
    37861 gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttga
    37921 tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat
    37981 caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat
    38041 tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta aagataagca
    38101 catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga
    38161 gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct
    38221 gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat
    38281 tctggttgcc actgatctga cgccgtccga aaccgcacag ctgaacctga agaaggtgct
    38341 gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct
    38401 ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta
    38461 tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga
    38521 taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa
    38581 agatctgcca gctattacgc tggacggtca ccaggtagaa gtgtgcgcta acattggtac
    38641 ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac
    38701 tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta
    38761 caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg
    38821 cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg
    38881 gcgcgctatc cgtatcgcga tggaccgtaa agagatcctg cgcgatcagc tccgcgctat
    38941 cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga
    39001 agaagtacgt gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg
    39061 taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac
    39121 aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca atgatttaac
    39181 gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat
    39241 gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa
    39301 atggactggc atgtgtggtg agcttgcagg cgatgaacgt gctacacttc tgttgctggg
    39361 gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat
    39421 ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac
    39481 aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc
    39541 cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga
    39601 aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt gctccgctct
    39661 ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg
    39721 gtgatggcat tgctatcaaa ccaactggaa acaaaatggt cgcgcctgta gacggcacca
    39781 ttggtaaaat ctttgaaacc aaccacgcgt tctctatcga atctgatagc ggcgttgaac
    39841 tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg
    39901 ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc
    39961 tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg gacgaaatca
    40021 aagaactgat caaactgtcc ggtagcgtga ctgtgggtga aaccccggtt atccgcatca
    40081 agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg cgccattttt tatgcttccg
    40141 ccagcggcgg caaaatcaat tcatcgctct catgctgctg ggtgtagcgc atcacttcca
    40201 gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc cttccctttc agcaatccac
    40261 tgataagctg agcacaaaac aggtcgccag tccctttcag gtcggttttt acccgtgaat
    40321 gggaaatgac attcacgctg tcggcactga ccaccaccac ctgcatctcc tgattttctt
    40381 cattaccgga ggcgctggta atcaccaccc attttaatgt gtctgaaagc agactttttg
    40441 cggcagcaat ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccagctcaa
    40501 agatattggg ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcctcgggaa
    40561 ggtcaggttt gacataaatt ccgctatcaa tatcgccaat caccggatcg accatgatca
    40621 ataggtcagg atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg
    40681 atgccgttcc catatagccc gtggttacag ctcgaagttg gcgcagcgca tcacgctcct
    40741 gaagcgcacg caaatagccg ctaaaccatt catccggaat cgcaccaccg tagaaagtgt
    40801 cataatgcgg tgtattgctc agcaataccg tcggcacggc aaagacattc aggccgttct
    40861 gtttgatagc aggcaccgca atgctgttgc ctacgctgcc gtacactacc tgcgactgca
    40921 cggcgacgat atccgcctgc aatgccctgc tcttatcgtt aaacaacaac aaactactca
    40981 tttaattttt tctccttgcc gatgatcctc atcgtattcc aaccgaaact ttacctgatt
    41041 ctggcagtca aatcggctat cacaaaacaa ggataaggta attcaatgaa gaaaatcatt
    41101 tgtctggtca ttacgctgct aatgacactc cccgtttacg cgaagttaac tgcccatgaa
    41161 gaagcccgta tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg
    41221 cgtaacggcg atgatcatac ctgcgatgaa gcggtttccc acctgcgtct gaagctcggc
    41281 aatacccgta accgcattga cactgccgag cagtttattg ataaggtagc ctcgtcgtca
    41341 tcgattaccg ggaagccgta tatcgtgaag atgcccggta aaagcgatga gaacgcacaa
    41401 ccttttttac atgcgctgat tgcgcagacg gataaaacgg tgcctgcgca ataatcttaa
    41461 atccccgccc cctggctaaa atgctcttcc ccaaacaccc cggtagaaag gtagcgatcg
    41521 ccgcgatcgc agatgatcgc cacaaccacc gcgccggggt tagcttttgc cacgcgcagt
    41581 gctccggcaa ccgcgccgcc ggagctgacg ccacagaata ttccttcccg cactgccagt
    41641 tcgcgcatgg tgttttccgc atcgcgctga tgaatatcca gcacctcatc caccagcgaa
    41701 gcgttgaaaa tccccggcag atattccgca ggccagcggc gaatgccggg aatgctactg
    41761 ccctcttccg gctgcaggcc aacaatggtc accggtttgg attgttcgcg cataaagcgt
    41821 gaaacgccgg tgatagtgcc ggttgttccc atgctggaga caaaatgggt aatgcgcccg
    41881 ccggtttgct gccagatttc cggcccggtg gtggtgtaat gggcgaaggg attatcagga
    41941 ttattgaact gatcgagcag ctttccttca ccacgattgg ccatctccag cgccagatcg
    42001 cgcgcgcctt ccatgccctg ctctttggtg acaagaatca gttccgcacc gtatgccagc
    42061 atcgctgcac ggcgctcctg gctcatgttg tcgggcatca gcaatttcat gcgatagcct
    42121 ttaagcgcgg caatcattgc cagcgcaatg ccggtgttac cactggtggc ttcaatcagg
    42181 acatcgcccg gtttgatttc tccgcgcttt tccgcctcga cgatcatcga aagcgctgca
    42241 cgatctttca ccgaacctgc cgggttattg ccttccagtt tcaaccagac ttcactgccg
    42301 ttatccggcg ccattcgctg caacttcacc agaggcgtat tgccaattgt ttgttctaat
    42361 gtactcacga tctctgtcca tacgtggtgt tgcctgatgc gacgcttgcg cgtcttatca
    42421 ggcctacagg ttacaaacct tgccattaaa aagcccggat cgcgggaagc gcctccgggc
    42481 gtttaacatt cactcaacct atcaggcgct ttgtgcgaga gcaagttcct catcgcgggt
    42541 ttcaatacgc tcgtcgccgt tatacagccg cgcatgttgc agaccaacga ataaacgctc
    42601 gccacgctgc ggggcatcgt cgccatgcat cacaaccgtc agcggttcgt tgtaccaccc
    42661 cagcggctgc acgactaatt gggtgtagtg gcctttcggg ctggcttcca gtacctgtac
    42721 cggcagcggc gaatcgaggc tggtacggcg gctgatatcc acttcccacg gacgcaggaa
    42781 gagatccacc ggcccctgat acgcaggtgt atagcccagc ggccagcgat gtgcgccaac
    42841 atggaactgc ccgccgcgaa tggttccctg caggcggttc acttcgccca taaattcgag
    42901 tacaaaacgg gtcgctggtt cgcgccatac ctggtccggt gcatcagcct gctcgatatt
    42961 gccctggctc atcaccacta cacgatcggc tacttcggtc gcttcttcct ggtcatgggt
    43021 cacaaacacg ctggtgaatt tcagctcttc atgtaactga cgcagccagc gacgcaactc
    43081 tttacgcacc tgcgcatcca gcgcgccaaa cggttcatca agcagcagaa tttgcggttc
    43141 tacagccagc gcgcgcgcca gtgccacacg ctgtttctga ccgccggaaa gctgtgctgg
    43201 ataacgatcc gccagatgcg caagctgcac catctccagc aattttgtca ctttcgcttt
    43261 gatggctgcg gcattcgggc gctcgcgacg cggcagcacc gtcaggccga aagcgatatt
    43321 gtcgaacacc gtcatatggc ggaacagcgc atagtgctgg aacacgaaac cgactttacg
    43381 atcgcgcgca tgcaggcggc tgacgtcggt gccgtggaag cgaatatgcc cgctggtttg
    43441 atgctccagc ccggcgataa tgcgcagcag cgtggttttc ccggaaccgg acggccccag
    43501 caacgcgacc atctgaccag aaggaatatc cagtgagata tcgttcagca cctgggtgcg
    43561 accaaaagac ttcttaatat tggcaatctc aatgctcatg atgttcctcc tgctgcgcgc
    43621 gtttttcctg attctccaga cgccactgca acatactctt taaaaacagg gtgataatcg
    43681 ccatcagcgt caacaacgcg gcagcggtaa aggagccgac ggtgttgtag tcttgctcca
    43741 gcaattcaat ctgtaacggc agcgaaagtg tttcgccgcg aatcgagccg gaaaccaccg
    43801 acaccgcacc aaactcgcca atcgcgcggg cgttggtcag caccacgcca taaagcagcg
    43861 cccagcggat gttcggcagc gtgacgcggc gaaacatctg ccagccagac gcgccaagca
    43921 aaatcgccgc ttcgtcttcc tggctgccct ggcttaacat caccggcacc agctcacgca
    43981 ccacaaacgg acacgtcacg aagatggtga ccagcaccat tcccggccag gagaacataa
    44041 tttgcagatt atgttcatcc agccagccgc ccagcgggcc gttagagccg tagaacagca
    44101 gatacaccag accagcaacc accggcgaca cggcaaacgg aatgtccaac agcgtcagca
    44161 atagctggcg tccggggaag ttaaagcgcg tcaccagcca ggccagcaga ataccgaaca
    44221 ccaggtttac cggtacggca atcagcgcga tcatcaccgt cagccagatg gcgtgcagca
    44281 tgtccggatc ggccagattc tgtaaaaccg gcatcagccc tttgctgaac gcctgtacga
    44341 agatataaat catcggcacc agcaggatga acgccgatac cagcatcccg atgccaatca
    44401 gaaaccattt gccccagtta atcgggcgcg cgtcataacg cttcaactgt gtcacttccg
    44461 ccattaatga cctaccacac gccgaccaaa gcgactttgc agagtgttaa ttgagaacag
    44521 cagcagcagc gatgccgcga ggatcaccga ggcaatcgcg ctcgccgccg ggtaatcaaa
    44581 ctcctgtaag cgaacaaaga tcatcagcga cgtcacttct gttttccagg cgatattccc
    44641 ggcgataaaa atcaccgcgc caaactcacc gaggctgcgg gtaaacgaca gcgccacacc
    44701 cgccaccagc gccggagata gttccggcag caccactttg cgaaaactct gccagcgtgt
    44761 tgcgccgagc gtttctgccg cctcttcata ttccgggcct aactcttcca gcaccggctg
    44821 cacggtgcgc accacaaacg gaatgctggt aaaggccatc gccaccgcaa tccccagcca
    44881 ggtataggtg actttgatat caaacttcgc cagccattca ccgtaaaaac cgttcacgga
    44941 aaagagcgag gccagcgtta aaccggcgac tgccgttggc agcgcaaagg gtaaatccat
    45001 cagcgcatca agcagcgtgc gacccggaaa gcgatagcgg gttaagatcc acgccatcag
    45061 cagaccgaaa acgccgttaa aaatcgatgc cacaaacgcc gacagcagcg tcactttata
    45121 ggctgcgacc acctgcgggt tggtgatcac ctcccagtac tgcgcccagc tcatctgggc
    45181 cagttgcatc accagcgcgg agagcggcag cagcaaaatc aggcacacaa acagcagact
    45241 ggtgccgagg cttaaggtaa agcccggcag cacgcgtctt gaagaaactg caaacattac
    45301 ttacgccccg ccgctaacag cttgtctaac tcgccgccgc tggtgaagtg ggttttcatc
    45361 acttccggcc aggagccaaa tttatcttcc acgcggaaca gctcggtctg cgggaattta
    45421 tctttcagtt tgcccatgac ttccgggtta tttacgcggt agtaatagtc ggtgatgatg
    45481 gtttgcgcct gcgggctgta gagccagttc aggtaggctt ttgccgcttt ttccgtgccg
    45541 ttggcctgta cgtttttatc cacccacgcc accgggaatt ccgccagaat gttggttttc
    45601 ggaatcacca cttcaaagcc ctgcgcttca tactgtttac ggatgttgtt cacttccgac
    45661 tcgaagctga tcagcacatc gcccaggccg cgctcggcaa aagtggtggt cgcgccacga
    45721 ccgccagtat cgaacacttc aacgtttttc aggaactggg tcataaactg ttcggttttg
    45781 gctttatcac caccgtcggc tttgtccgct gcgccccatg ccgcaaggta ggtataacgc
    45841 gcgttacccg acgttttcgg gttcgggaaa atcagcttca cgtcagagcg taccaggtcg
    45901 ttccagtcgt ggatattctt tgggttgccc ttacgcacca ggaagcccat ggtggagtag
    45961 aacggcgagc tattattcgg caggcgcgac tgccagtccg ctgggatcag cttgccttta
    46021 tcgtgcagga tttgtacgtc ggtcacctgg ttataagtga caacgtcggc ttttaaaccc
    46081 tgcaaaattg ccagcgcctg ttttgatgat ccggcatgag attgtttaat cgtcagtttg
    46141 tcgccaccgt tatcttttgc ccattgttgc tcaaacggcg ggttcagggc ggcaaacagc
    46201 tcgcgggaga cgtcataaga actgttcagc agttccgttg cctgtacatg gcccgccagt
    46261 agcagagaag caaccagcac gagtgagttc tttttcagta agttaacggc cattgcgcac
    46321 ccttataaat ttaatgactt tctaatagcc atcatattta taacggacgt gaaaggagta
    46381 acggttttat ataccgtttg gtgatttgga agttgaaaag ggaataagac gaaatcctcc
    46441 ccacaaatgc agggaggaaa cagaggtgaa tcagatgccg acgctaaccg tctccggcag
    46501 tgtgctgccg ccatcaatca cattctgtgt accggttaaa tagctggatt catccgatgc
    46561 gaggaaggcc gccagttcgc cgacttccag cggatcggcg aggcgacgca tcgggattgc
    46621 tttcgccatt tcagtcagca ccgattctgg atcttccggg ttcgactggc gggcaatgct
    46681 ttccgccatt ggcgtgcgaa cgtatccagg gcagatggcg ttaacgcgaa taccagactg
    46741 cgcgtactcc accgccagcg atttagtcag gccaacaatc gccgctttcg ttaaggcgta
    46801 cgccgtttcg cccgggtctg ccaccatatc gccagtgact gaagacatca tcacaatccg
    46861 gccatctttg cgggcaatca tctccggcag caccgccttc gtgacgttcc atacgccttt
    46921 tatattgata tcaatatgga aatcgcgatc ttcatcgctc atatcaagga aactgcccag
    46981 acgacaaacg cctgcgttat tcaccagaat atcaatgcgc ccttcttttt ccttagcgcg
    47041 cttaatagct gcggcgaccg acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg
    47101 atgaccacgg ccacacagtt cgtccgccag cttttcgatc tcaggggaga tatccagcaa
    47161 gattaggttt gcgccatgac gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa
    47221 tgcgcccgta atcagtgctg tcttgcccgt gagtttaccc attttaatgc tctccttgtt
    47281 aatacagagc gttacactcc attccctctg aaaatacagg ataaacaaaa tattgctgcg
    47341 gatcagctca ctaattcagg aaaatctcaa tccagtcgct gctttatgca tcatttcagg
    47401 cgttgcgtta gctcactgct gcgctcaatc attttcaatg actccacatc attgagttgc
    47461 acaaggccaa caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt ggacatcgac
    47521 gagctgcgcc attcggtttc gccagaaacg gtatcgaggg tgtaatgcgc cagccgccgc
    47581 agcggggaat cggttaatga ggtaatggca atcaccgttg ccccctgctt tcgcgctgcc
    47641 tcggcgcaca agacaatctc ttttttactg ccactgtaag agatagcgat ttgtacatcg
    47701 ccttttttta gcgcctgaga aacggtggct tgtacgtggg tgtcggcctc acaggccacg
    47761 cgataaccaa ttttcatcag tttgaacgac agatcgcgcc ccaccagctc cgacccgccc
    47821 aggcccgtta tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc
    47881 gcgtagtcaa acaacgcgca ggtttgctcc agcgcctgct ctttttcgcg attcagttta
    47941 cgggcaatca cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg
    48001 gcattcgttt tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta
    48061 aatccttgcg cgccgagctt ctgggcaaac ttcacaatac tcgactggct gatgccgagc
    48121 tgtttcgcca tcttgcgcga agagacggat ttcagctcac tcacgttggc ccgcagaaag
    48181 tcagcaattt tttgctcgtt cccggtaaat tcactttccg cattgcgtat tttcgtcagg
    48241 tacaacatct tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc
    48301 tcttcacact ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata
    48361 tgattccata catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaactt
    48421 gaaaagatga ttaccgaagg ctcgaacgcc gcttcggctg aaattgaccg cgtatcaacg
    48481 ctggaaatgt gccggattat caacgatgaa gataaaaccg taccactcgc cgttgagcgc
    48541 gtactgccgg atatcgccgc agcgatcgat gttatccacg cccaggtcag cggcggcggt
    48601 cgtctgattt atctcggtgc gggaacatca ggtcgtctgg ggattctgga tgccagcgaa
    48661 tgtccgccga cctacggcgt gaaaccgggt ctggtggttg gattaattgc tggcggcgaa
    48721 tatgccattc agcacgcggt ggaaggcgcg gaagatagcc aggaaggcgg tgtgaatgat
    48781 ctgaaaaaca tcggattaac cgcgcaggat gtggtggttg gtattgccgc cagtggtcgc
    48841 acgccgtatg tgattgccgg actggaatac gcgcgccagc tcggctgccg cacagtggga
    48901 atttcctgta atccagggag cgcggtttcc accaccgctg agtttgctat tacgccgatc
    48961 gttggtcccg aggtggttac cggttcttca cgaatgaaag caggcacagc gcagaaactg
    49021 gtgctcaata tgctctccac cgggctgatg attaaatccg gcaaagtgtt cggcaaccta
    49081 atggtcgatg tggtcgccac caacgaaaaa ttgcatgtgc gccaggtcaa tatcgtcaaa
    49141 aacgctaccg gatgtagcgc agagcaagcg gaagcagcgt tgattgcctg cgagcgcaac
    49201 tgtaaaacgg ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc
    49261 ctggatcaac acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa
    49321 gagatcagca gtgaacttct gaacaccatt cttacccggg tcggtggacc gggaaatatc
    49381 gccagttgtg gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt
    49441 gatcccaata tcaaaacgct ggaaggcgtg aagggcgtca ttttaaccag cgaccaggtg
    49501 caggtcgttt ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg
    49561 ggagaagcac cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa
    49621 gccaaacaaa cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg
    49681 ctgatccccg gttttattgc cgctggtctg ctgctgggga tagcgacgtt aattgccacg
    49741 gtgatgcacg ttccggcaga tgctcagggc acgctacccg atgcgctgaa ttttatgaag
    49801 gtgttcagca aaggcctgtt caccttcctg gtgattctgg tgggctataa cgccgcccag
    49861 gcatttggcg gcacgggcgt caatggcgca attatcgccg cgctcttttt gctcggttat
    49921 aacccaactg caaccaccgg ttactacgcc ggatttcacg atttctttgg tctgcccatc
    49981 gatccgcgcg gcaatattat cggcgtgttg attgccgcgt gggcctgcgc ccgcatcgaa
    50041 ggcatggtgc gccgctttat gccggacgat ctcgacatgc tgttgacctc gttaatcacc
    50101 ctgctaatca ccgctacgct tgcgtacctg atcattatgc cgctgggcgg ctggctgttt
    50161 gaaggcatgt cgtggctgtt tatgcacctg aacagtaatc cgctcggttg tgcagtatta
    50221 gccgggctgt tcctgatcgc cgtggtgttt ggcgtgcatc agggctttat tcccgtttac
    50281 ctcgcgttaa tggacagtca gggattcaac agcttatttc ccatcctttc aatggcaggc
    50341 gcgggccagg tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta
    50401 cgcagccagg tgcgcggggc gattattccc ggtctgttgg gcgttggcga accgctgatt
    50461 tacggcgtca ccctgccccg catgaaaccg tttgttaccg cctgtttagg tggcgcggcg
    50521 ggtggtttgt ttatcgggct gatagcctgg tggggcctgc cgatgggctt aaacagtgcc
    50581 tttgggccgt ctggtctggt ggcgctgccg ctgatgacat cagcacaagg tatccttccg
    50641 gcaatggcgg tttatgcagg cgggattctg gtggcatggg tttgcgggtt tattttcacc
    50701 acgctctttg gctgccgtaa cgtcaatctg gactgataat atgaaacgga caatgctcta
    50761 tctttctctg ctggctgtca gctgtagcgt cagcgccgca aaataccctg ttctgacaga
    50821 aagttcgcca gagaaagcag ggtttaacgt cgaacggctt aatcagatgg atcgctggat
    50881 aagccagcaa gtggacgccg gttatcccgg cgtaaacctg ctgattataa aagataatca
    50941 gattgtttat cgcaaagcct ggggcgcggc gaaaaagtac gatggcagcg cgttaatggc
    51001 gcagcctgtc aaagccacca ccgggacgct gtatgacctg gcttccaata ccaagatgta
    51061 cgccactaac ttcgccctgc aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg
    51121 gattgcgaaa tatattccag ggtttgccga cagcccgaac gatgcgatca aaggcaaaaa
    51181 cacgctgcgg atttctgatc tgctgcatca cagcggcggt ttcccggcag atccgcaata
    51241 cccgaataaa gcggtcgcgg gcgcgttata ttcccaggat aaaggccaga cgctggagat
    51301 gatcaagcgc acaccgctgg agtatcaacc ctgcagcaag catatctaca gcgatgtcga
    51361 ttatatgctg cttggattta tcgtcgagtc ggttaccggt cagccacttg accgctatgt
    51421 tgaagagtcg atttatcgcc cgctcggcct gacgcacacg gtatttaatc cgctgctgaa
    51481 aggctttaaa ccacagcaaa ttgccgccac ggaactgaac ggcaataccc gcgatggcgt
    51541 gattcacttc ccgaatatcc gcacttccac tctctggggt caggtgcacg atgaaaaagc
    51601 cttttattcg atgggcggcg tttccgggca cgcaggtttg ttttccgata ccggcgatat
    51661 tgcggtgtta atgcaaacga tgctgaacgg cggtggctat ggtgatgtgc agctgttcaa
    51721 tgcggaaacg gtgaagatgt tcaccaccag ctctaaggaa gatgccactt ttggccttgg
    51781 ctggcgcgtg aatggtaatg ccaccatgac gccgacgttt ggcacgctgg caagcccgca
    51841 aacctacggt cacactggct ggaccgggac ggtgaccgtt atcgatccgg tgaatcatat
    51901 ggcgattgtg atgttaagca acaagccaca ttcgccagta gccgatccgc aaaagaatcc
    51961 caatatgttt gaaagcggtc agttgccgat tgcaacttat ggttgggttg ttgatcaggt
    52021 gtatgcggcg ttaaagcagt aataaaaagg ccagtcagtt gtgatactgg ctggccttta
    52081 atcatcgaat cagaaacgct tacaacgcca ttaacttgtc cagcgacggc gcgaagtaat
    52141 agccgccagt gaccggtttg gtaaaacgca acatcgcatc acgtttgcca tcggtatcgc
    52201 caaacatgct cagcagttgc tgctcaatgt tatgcagacg cgcgcagtag gcgcagaagt
    52261 acagaccgtg cgtaccgctg gcggtgccgt acggcaggct ctgtcgaaca atcttcagcc
    52321 ctttgccatc ttctttcaga tcaacgcggg tcaggtgaga ggtttccgga cgttcgtcgc
    52381 cgtcaatctc ttcgttggcc tctttagtgc gcccgatcat catctcttga tcgtgaatgc
    52441 tcatccggtt gagttgcttc aggttgtgct cccaacgctg cacaaacaca tagctgccgc
    52501 cggcatccac gccatctttg ataaccgcca cttcgcggcg cgtctcttca cccgccgggt
    52561 tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc aacccaacgg aagccgtgga
    52621 tctcttcttt cacttcaatg caatcaccaa acgcttccat cgccgcctgg gcgacagaga
    52681 agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac atcgaactgg gtcgtcggtg
    52741 caagaccttt accgtagccc ggaaaatctt tcagctcttc ggccccaacg ccgccgctca
    52801 gagcgcgcca ggtattgtta ccaaaggcaa ccaccgcacc aagatgcgcg tccgggaatt
    52861 tcgcttcaaa agttgccagc ttgtcggcaa atgttttact ggccgcacgc agggcgtcaa
    52921 cttccccttt cacgttggct tcgatccaaa tcgccgcgcg gcaatgttct ggcaaaatgc
    52981 cactctgaac ctgagacatt gttcctcctg aaaataagaa tgccacgcaa tccgtggcat
    53041 tgttgggcgc tattttacgt gttttttcgc ggcggcggtt tgttcagacg caaattaacg
    53101 ccgccagata attttactga ctttccagtt tttcagggta tcgtccgaag gcattaaccc
    53161 ttcaggacca ctccattccc cgctgaactg gtagctaata tgttgactgc cttcggcttt
    53221 acattcgacg gcacgattat catcaccatc agctttttgg caattgccaa atgctttgct
    53281 gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg tcagcaggaa tatcgctatc
    53341 cagcacgtca atgcgactga tcgtgccttg atcgccgtta atcaccatcg ccacgttgtc
    53401 gcccttcatc acttcaaaaa agcgcaccac gttgccgttc gcggttttca ttccgctgcg
    53461 caggcgataa tcgccatcaa gcgcatcggc aatggcttgt tcttgcagtg gtgtggacgc
    53521 cgttaattca ccaacgccct gctcgctcac tttggtggat gatccaaacc agttccacgg
    53581 attagcggca gaccagttaa cggacgacat cgtggaacag ccggtcagca tcaacggcat
    53641 agcgcataac attaaacgca gcgatttcat gtcacttcct ttggttattc aataacgttg
    53701 cttggagtgc aaattcacca aaaagtgccg ttattctttt tcttcattaa aacaagcgcg
    53761 taaacgtcga ttggtcagca gccagattaa cgccacaata tccgccacta ccagcgccag
    53821 accgataccg ctaacggatt caccgttcag ccacaaccac ggctgccagc aaagtaaaac
    53881 cacttgcgcc aacagcaaca gaaaatagag cacatgccag atacggggaa atgaagcccg
    53941 ccgaccactc agcaaaaacg ccagcaccgc cggaatgccg ggaatcaacc ccagccagaa
    54001 attatcgtga tcgggataaa acagatttag cagtgcagta ccctgctcgc gcgacgcacc
    54061 ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat agcacaagcc agaagagcaa
    54121 gggtaaacgc aggcgaccgt acgcatcata atggacagga tgaaactcag tactcttcat
    54181 cttcaatcaa acgcttaccc agactcagca cgtcggcgtg ttcatatccc aggcgttcat
    54241 acattccgag caccatgtcg ttatcttccg gcacgttgat ctgaattttc gggcagccgc
    54301 gagcaatcag ctttttctcc aaccgattaa gcaacgcatt agcaatccca cgcccacgaa
    54361 actctggatg cacgccaaga taataagcag acccgcgatg cccgtcataa ccgcccatca
    54421 ccgttccgac cacttcaccg ttcacctcag cgaccagaaa caaactgacg tcatggttca
    54481 tcttacgctc aatgtccatt tccggatcgt tccacggacg cagcaagtcg caacgctccc
    54541 aaagggtgat gacctcttcg aaatcttcct ggcgaaatac gcgtatctcc atggtattcg
    54601 ttaccttttt gcgggttaaa aggctgatta tggcgtgaac ggtcgaatta gccaatatct
    54661 gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt caactattga aatgaaaagt
    54721 aaaacaattc tcaacagcaa acggtcgtaa cggattacgc gatacgatat aacatctgga
    54781 actttattat tacaactcag gccgtatgag cacttttaaa ccactaaaaa cactcacttc
    54841 gcgccgccag gtgctgaaag caggacttgc agcgctgacg ttgtcaggaa tgtcgcaagc
    54901 catcgccaaa gaagaacctt taaaaaccag caacggacac agcaagccga aagcaaaaaa
    54961 atctggtggc aaacgtgtcg ttgttctcga tccaggccac ggcgggattg ataccggagc
    55021 gatcggacgc aacggttcaa aagaaaaaca tgtggtgctg gcaattgcca aaaacgtccg
    55081 ttccattttg cgtaatcatg gcattgatgc gcgtttaacg cgttctggcg atacgtttat
    55141 cccactgtac gatcgcgttg aaatcgccca taaacatggc gcagatctgt ttatgtcaat
    55201 tcatgccgac ggctttacca acccgaaagc tgccggtgct tcggtatttg ccctctctaa
    55261 ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa cgcgaaaacc gcgccgatga
    55321 agttgccggt aaaaaggcga ctgacaagga tcacctattg cagcaagtgc tgtttgatct
    55381 ggtgcaaacc gatactatta aaaacagcct gacgctcggc tcgcatattc tgaaaaagat
    55441 taagccggtg cataaactgc acagccgcaa caccgaacaa gccgcgtttg tggtgttgaa
    55501 atcgccgtcg gttccttcgg tgctggtgga aacctcgttt atcaccaacc cggaagaaga
    55561 gcggctgtta ggcacggcgg catttcgcca gaaaatcgcc acagcgattg ccgaaggcgt
    55621 gatcagttat ttccactggt ttgacaacca gaaagcacac tcgagaaagc ggtaagtgat
    55681 gaaacccgac gcacaccagg ttaaacagtt tctgctcaac cttcaggata caatttgtca
    55741 gcagttgagc gccgtcgatg gcacagaatt tgtcgaagat agctggcagc gcgaagctgg
    55801 cggcggcgga cgtagccggg tgttgcgtaa tggtggtgtt ttcgaacagg cgggtgtcaa
    55861 cttttcacat gtccacggtg aggcgatgcc tgcttccgcc accgctcatc gcccggaact
    55921 tgccgggcgc agtttcgagg cgatgggcgt ttcactggta gtgcatccgc ataatccgta
    55981 tgttcccacc agccacgcga atgtgcgctt ttttattgcc gaaaaaccgg gtgccgaacc
    56041 cgtctggtgg tttggcggcg gcttcgactt aacccctttc tatggttttg aagacgatgc
    56101 tattcactgg caccgcaccg cccgtgacct gtgcctgcca tttggcgaag acgtttatcc
    56161 gcgttacaaa aagtggtgcg acgactactt ctacctcaaa catcgtaacg aacaacgcgg
    56221 cattggcggg ctgttctttg atgatctgaa cacgccagat ttcgaccact gttttgcctt
    56281 tatgcaggcg gtaggcaaag gctacaccga cgcttattta ccaattgtag agcgacgtaa
    56341 aacgatggcc tacggcgagc gcgagcgcaa tttccagtta tatcgtcgcg gtcgttatgt
    56401 cgagttcaat ctggtctggg atcgtggcac actgtttggc ctgcaaaccg gcgggcgcac
    56461 cgagtctatc ctgatgtcaa tgccgccgct ggtacgctgg gagtatgatt atcagccaga
    56521 agatggcagc ccggaagcgg cgttaagtga gtttattaag gtcagggatt gggtgtaact
    56581 ccctcccccc cattcccgca tccgctgatg cagcgtcagt gacggcttct cggcaaacag
    56641 ctgctggtaa tccgtggcga attgccccag atgccagaat ccccactgca tggcggcgtc
    56701 ttttaccgtt gtgctttgcg accaagggct tatcagttcg cggcgtacgg cgttcaggcg
    56761 aatgcgtttc agccacgcgt ttgggccaat gcctaaaata gcgtgaaacg cgttttgtag
    56821 cgtgcggcgg ctgacatgca gttgattaca caagtccagc accgtcactg gttcggacat
    56881 gttttccagc acatattcac gggcgcggga aagcaatcga cggtaactct gatgactgat
    56941 gctttccgcc gtcaccattg gctgcgcttc ttccagcatc gcccccatcg ccattagcaa
    57001 attatccccc agcacttttc gcactgcagg ctgatggaga ttttccgggt tttcgcaaaa
    57061 cgtcgccagc gcctgttgga caaagcccca cagagcggct ttatgctgct ctttcacttc
    57121 caacgccgac tggctacgca gcatatgtaa tacccgatcc ggattatgca aaaagttagc
    57181 ctgacgagtg atgacatctt cagaaagcac tacgccaagg atcgtgtaat cgtccggcgt
    57241 acttaattca aactcggttc caccggggcg cgtcgctatt tctgcgcttc ccagacattg
    57301 cgaaccgata aatccctgct caccgcgcgt cgccggaatg ccaaaccaga acgagttcgg
    57361 ccagaccagg cacgactggc gcagcgccag accggtgtat tcacggaaaa cctgaatatc
    57421 atcgagtaga atttccgtaa attcaccatg aaacttgccc ggatgcagct gatcgtaaat
    57481 ctgctgccag gcggtaattg tcaaagcatg ttcatagaca tccgttgtct gtcgttgatg
    57541 aacattatcc acttcgacct tcggcgtgag cttcaggttt tcgggtaagg gttcatgata
    57601 aagatggtgc aaattggctg tacgggtctt tttcatgatg ttaatgccgg gtgttgtagg
    57661 acacccggca cctccgacag gttaatgggg cttgagacga taacgactac tgcgtttacg
    57721 taacgtcccg gcagaaaaga gctgttcgag cgcatttctg gctttttcca gcggccagcc
    57781 aaagtgggca gccacttctc ctgccgtcat tccctgacgt actgatgtta acagcgccaa
    57841 caactcgtca gcagattccg cgacgataac tggcgcgtcg ggttccttta cgggttgctt
    57901 aggctggcgg ttaaagccag taaccagcca ctgggtgtca tcgtctggcc gaccgatctc
    57961 cttacggctg atgacacgcc cggtacgcat cgcggcagca caacctgcgt ccagcgccgc
    58021 acgacacgcc gccagatcgc cttccaccac cagcgttagg cgaccagggt caagcacttc
    58081 gtgactgagc agacgaacgt tagctgcttt gagcatggca tcagctgcat ctatcgcggc
    58141 gaccatgccg tccacttcca gcaatcccag tgcattgatc atcggcaacc tccgttacgc
    58201 acgctggatt ggattacgcg cgatttccag cactgcatcg gtaaaggcgt tacaggctgc
    58261 tttacacgcg gcctggctac cggttaaaaa tgctgccgag tagttggttt cagacggtgg
    58321 cgggacatag gtcaccagct gtacgtcggc agatttcagg gctgcatcaa tgccgtaggt
    58381 cgcttccagc ggcggtgcca ccagatacgc catcggatcg ccaagcgtga tcccggcggt
    58441 tgatgagaga taagaaccgg tacgcgaaac tacatgtgcc aggaaggctg tgttttctgc
    58501 gtcattagcc cactggaaag cagcgccatt ttcaatatgc gcaaccatcg catccagacc
    58561 agcacgcact tccgccgggt ttggcccacc aagcataatc agcacttcac cggcagtcgg
    58621 tgacgggccg tgtgcggctc ccgcatacag cgagcggcca tacaccactt ccaccatcgc
    58681 ctgcttggtc gcttcgtcag ccgcaatgta ggtgacgtca tctgaatcag cagaaatcag
    58741 cccgaggcta cgaatatgcg gcggcaattt cagttcacgc gcaaattcgg cgttaacgga
    58801 ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa tctaaagctg gcatgatgcc
    58861 tccttatcgg gtcatgttga tgccggatgc tttctgctcc agcatacgtt tcgccaaatc
    58921 cacaatgacg gctgcggctt caaccggcgg cgtgccgccc tggtgaatgt tagagataca
    58981 ggtacggtcg gcctcaacgg tggttgccat acgcggcgag taaacggcgt agcaggagag
    59041 gctttctgac tgccctaatc ccgggcgttc ccccaccagt aggatcacta ctttcgcgcc
    59101 gaggatctca ccgatctgat cttcaatctt cacgcgacca taacgaacaa agaatggtgt
    59161 accgactttc agcccggcct gtttcaggcc cgccatcagc ggtggcagga tctcttcgta
    59221 gttcacggtg atcgcatcag ttgacaggcc atcagaaata acgacctgta cgtccggatt
    59281 agcaacacac tgtgctttca gcgcttcaac agcttctgca cacaggcggc ggcccatatc
    59341 cggacgagtc aggtagagat ttttgtcgct aatttcagag cggacttcca gcaagccctg
    59401 cgctttcacc cactcttccg gtacttcttt cagtacggta tctttcgaac gagagtgatc
    59461 ggccaggaaa cgcagcaacg cctgggtacg cggacgcgga ccggcacgac cggtacaaac
    59521 gcgggccacg gtgctgcgac gcagttctgt taatacgtct gcgcgatgcg ggttttccac
    59581 accaatccac gcttttgctt ccgcagaacc taaatccagc gcgcagcttt ccgaggtcac
    59641 tggtgtcgta caggtggtgg tggcgcactt tgcttctgac ggcgcggggg ccgcttgtcc
    59701 cattgacgcc atcacgctgc gtacaatttc ttcaatctgt ttttgatcca tgatgtgtta
    59761 tctccgcgtc atcagaagaa cagtgacgga tcgcccgccc gtttggtcag gcgaccgttt
    59821 gccataatgc ccatgctttc cagccagcgt tcaaactccg gtgacgggcg caggttgagt
    59881 aactgacgca cagtggcggt gtcgtggaag gccgtagtct ggtagttgag catgatgtca
    59941 tcacccagcg gcatccccat gatgtagtta cagcctgcgg tggcgagcag gatcatcagg
    60001 ttttcgttga ggttctggtc agcgtcagcg tggttggtgt agcagcagtc acagcccata
    60061 gagatgccgc tcagcttgcc cataaagtga tcttctaagc ccgcgcggat aatctggcgg
    60121 tcgttgtaga gatactccgg cccaataaaa ccgaccacgg tgttgacgat aaacggatcg
    60181 taatgacgcg ccagcccata gttacgcgct tccatcgtca cctggtcagc gccgaagtta
    60241 gcgccagcgg acagcgccga accttgtccg gtttcgaagt agaggcagtt ttccccggcg
    60301 atacggttaa actctgcacc cactgcgcgc gcttcgtcga gcatcgccag ctccacgcca
    60361 aactctttca gccctttttc gctgccgcaa atactctgga aaatcagccc gcccggtgcg
    60421 ccgcgacgaa tcgcttcgat ctgggtggtg acgtgcgcca gtacgcagcc ctgagttggg
    60481 atgttgaatt tgtcgatcac gccatagatg gtatccaaca cgcggcttaa gttttccacg
    60541 tcgtcagtca ccgggttaac gccgatcacc gcatcgccca ccccgaagga aagcccttcg
    60601 tagatttgcg cggcgatact ttgcacatcg tcacgggtat cgtttggctg caagcgggcg
    60661 ctaaaggtgc ccggaatacc gatggtggta ttggcctttt tgatcaccgg cattttcttc
    60721 gcgccgtaga tcaggtccgc gttggagcaa atcttcgcta ccgccgctac cacttccgag
    60781 gtcagccctt tgcgggtaaa ggcaatgtcg tccacgctgg tttcatcgct cagcacatac
    60841 tcacgcagtt cgctgatgct ccagttttta atctggttgt aggccgtttc gttaacatcg
    60901 tcctgaatca gccgcgtcac gcagtcatct tcataggcaa tcaccggatt attgcggatg
    60961 tccgctacgg tcatttccga caacacctgc tttgccgcca cgcgctcctg tgagcttgcc
    61021 gcagcaacgc ccgccagcac atcccccgaa cgcagttcgt tggctttagc cagcacctct
    61081 tttacatcct taaactgata tacattgccg aacaatgtgg tctttagttt cataagtcgt
    61141 tccctcagga aggaaatgcg agtgatttca ccgtcaccgg cacaaccgat ccgccaaaaa
    61201 gaggcgtacc aatgtcgata tagtcccccg cgcggacaat cacttcgtca atgactgcca
    61261 acgggagttg ttgtagctgt gggcgcaaca acatgcccag agctttacca aagtcctgcc
    61321 cggccaccac cagcagggga tgcggattcg gaaaacgcgc gacgaaatcg accaacgcgt
    61381 tgatgaccgt cagtaccgcg gcgtaacgca caggcagcga ggcgggaagc gccagcacgt
    61441 acgcgtcagt tttaggatca agatccagct gaatcagcgc ctgttgccag gcactcacca
    61501 gatccttttc atcaattggg atcgccaccg gcaagttgcg cagcggcagt tgtacgccct
    61561 ccagccagat tgtgctgcca gagagcgaaa gggtatgtgc acccgcgcca atcaccgtgg
    61621 cgcgcacggt ttgcgccgga aactgcacgt tcatctcacg caggcgcgga tggtcatgca
    61681 gcgccgttgc cagaagcggg ccaatatcgg caaaacagaa cgggtcggcg ggctggtggc
    61741 gataacattc gccaacgccg ccagaaagcg taatgatttc gggcgtaaca cctacgggca
    61801 gcaaaccggt ttgcatcaat gcctgcgcga gcggcgaaag cgttccgtca atcacttcga
    61861 caatcagctc tgccatccgc cgggtaacct gcaccagctg cgcgccggtc agcgaacggg
    61921 cttcggtgcc tgcaccgaag cactcatcca caatcatctg ccccggttta tgagcgtaaa
    61981 ccacacgccc ctggctgtcg gtttccagca ggcgaccgcc gacattgagg caggcggtgc
    62041 cgctgatttt tccggcatcg aacagggcgt agttcgcggt gccaccgccg atatcgatat
    62101 tcaggacccg acacagccgt tgttcagaaa gggtttgcgc cccggcaccg tgaccggcga
    62161 tcacggattc gagatgcggt ccagcgctgg cgacgacaaa atcgcccagc gactgggaga
    62221 gcgtcatcac cgccgggcga gcgttgcggg ttttcgcgct ttcaccggtg atgatgatgg
    62281 caccagaatc aacgctttcc ggcgcaatgc ccgcagcctg atattgctcg aggattaagg
    62341 ttttcagttc cgcttctttt aaaccaccct gtttatcgac aggggtaaag aacaccgggc
    62401 tttgccagct aatttcgcgt ttgatgaatt cgtagcgcgg cacctgcgac accgccgcac
    62461 ggttaaccag ctccaggtgg gagaaaatca cctgggtggt ggtggtgccg atatcgatac
    62521 cgacgctcag tagctggcga gtgttcacga ttgcgcctcc gcttcggttt tagccgcgct
    62581 cgcgtcttct ttaggtacca gcatcatcgc cacgccaatc gccgtgacgc cgccgatcag
    62641 cttgccgaca atcatcggga agatcatggc gttcatgttg gcagccgcga agcctaaatg
    62701 gtcgcccagg gcgaaagcag cggaaacggc gaaggcacag ttgatgactt tgccgcgggt
    62761 atccatctgc ttcatcatgc caaacatcgg gatgttgttg gcaagcgttg ccaccatgcc
    62821 ggctgccgct atattgttca tattcagcac cttaccgaca ctcatcagcg gtttttcaaa
    62881 ccagcgagtc agcagcagca ccatcggata cgcccccagc agcacgcagg agatagaacc
    62941 gataacttca atggcgcgca tcacttcacc gggtttgtcg cctggagcca tgaagatcgg
    63001 atccagaccg gggatcagtt cccaaccgag caggaatttc actaccgcag cggcaagacc
    63061 gagggtgatc aatgcaacga ggaatttggc gaagatctgg aagccgttga tcattttttc
    63121 cgggatgaat ttcagcccca gcgccaccag caccgcaacg ataagtaccg ggatcatgtt
    63181 catcaggatc agcgcaaagg tgaattccac cggctgaccg ttgatctgca caccggagta
    63241 catagcaacc agaccaccag caatacagcc aatcggaatg gtcacaatgc ccgccagcac
    63301 gccgagcgcc agataacgac ggtcagaagg ttcgataatg ccgagcgcca ccggaatgga
    63361 aaacacaatc gttggcccca tcatcgaccc gagaattaac ccagagtata gccacgctgc
    63421 tacgtcgccg cccgccagct ctttggcgag gaagaagccg cccatatcgc acgccagcag
    63481 tgttccggcg aacatcgatg ggttagcgcc gagcatttcg taaaccggga taatcaccgg
    63541 cccgagtacg tgggccagta ccggtgccag cgcggtcata ccgaccatag ccaggcccaa
    63601 agcgcccatt gccataaagc cttcttcgaa ctgaccgccg gatccttcga tacttttacc
    63661 gaacttaccg aggaaacgag cagaaccgcc gaactgcgac aggatcctgt ctacggcagc
    63721 tatcagcata aagaacatca tgatgtacat gatgatttcg ttaataccca tcgcctttac
    63781 tccctgttag ttgttattta ttggcggatg cggcgtgaac gccttatccg ccctacatgt
    63841 gcaatcccga aggccagata agacgcggca agcgtcgcat ccggcaattg caccgcgcca
    63901 ctggcggatg cggcgtgaac gccttatccg ccctacatgt gcaatcccgt aggccggata
    63961 agacgcggca agcgtcgcat ccggcattta ttgcgccgct gcgtacaggc cgacaatctg
    64021 ctccaggctg gcggtacgcg ggttactgcg cagacaaata tcttccagcg cggcctgcgc
    64081 ccatgcgccg taatgcgcag atgtcgcacc aacatcgccc agtcgtttac caatcccaac
    64141 ttccgctatc agctcactta ccgcattaat agcgtcacga tcgtcggatt ttttagttcg
    64201 cagcgcccga ccaaccaagc taaaccgatc acgacaaacc atccggttaa attccatcac
    64261 cgttggcaac aacatggcgt tcgccagacc gtgcggaata tgcagcgccg cccctggctg
    64321 atgcgccatc gcgtggcaca acccaagtcc ggcactggaa aacgccattc ccgccataca
    64381 tgaagccagc aacatgctct cgcgcgcggc aaggtcatga ccgtagccca ccgctttcgg
    64441 cagcgatttg ccaatcatcg caatggcacc aatcgccaga ctgtcggtaa acggtgtggc
    64501 gttcagcgcg ctgtacgctt caatggcatg ggttaacgca tcaatgccag tcatcgccgt
    64561 gacatgcgac ggcacacctt cggtcaatgc ggcgtcgaga atcgccacat ccggcatcag
    64621 cgaggcatgg gctaacacct gcttgcgccc gctcaccgcg tcgataatca ccgttacgtt
    64681 ggtggtttca gagccggttc cggcggtcgt tggaatggca atcaacggca ggcgcggttg
    64741 cagaacgctg gtttctgaca tttctgccag cgtgctatcg gggttcgtca ccagcaacgc
    64801 cacggctttc gccgcatcca gcaccgagcc gccgccaaac gcgatcaccc cgtcacaacc
    64861 tgattcacgc aactgcgcca cggctgcaca cacgtcggtg atgcacggtt cgcccaccgg
    64921 acatggccag agcgtcatgg cgataccttt aacggccagg ctacgcgtca gcccggcggt
    64981 catccccgcc tgatgcaaaa agctgtctgc catcacgaac agatgtttca gcccacgcgt
    65041 ttgcgcttgc tgcccacaac tgctcaccgc gcccggaccg caaagcgtca ccggtggaac
    65101 gctaaatgtt ttcacccgct gcagattcag ggtatcgaat gcctgaaaaa gcgccgtttg
    65161 caattcactt tgcatagatc ccctccgttt tctctcgccc actactggcg atcgccagcg
    65221 gggtcataaa caaactgtgc tgcggtaaat gcacctgtaa cgccgggaat tgtttgcgaa
    65281 acagttccgc tacgcccggt tgcatacagg aaccgcccgc cagccataaa tccgtaatgc
    65341 cctgcccttc aatatgccgg gccacaatgt ccgccatttt ttcgtacacc ggtttcaccg
    65401 cgggccaaat ctcgtcaccg tgaccgcgtt tgtactgctc tgcctcttcc agcgaaatac
    65461 ggcgatttcc ggcgagagtc agagagatgt gatgtccgcc ggtggcttca tccgccgagt
    65521 acgtcacttt gccctttttc acgatggcaa tgccggtagt gccgccgccg atatccacta
    65581 caccggcgtt gtccagttgc agcagatccg caactgcggt tggctcatcc agcacatggc
    65641 tcacttccag tccagcagac tccagcacgt tgatggaaat acgcgggtcc gtacctggag
    65701 gaaaagaggt cgccgcatgg ctaaaacggc gaccgaattg ctgctcgagg gtgtcgagat
    65761 ggcgacgaac aatggtgacc gcaccgaaga aatcccagac gatgccgtcg cggaccacat
    65821 cagcccagtc gaggcatacc gccaccggct gaccgtcgcg gtcgacaacc atcgacacca
    65881 catcgcaggt gcccagatcc acccccagcc acagcggaga ttccgtcgcg gcgggcgtct
    65941 ggttacacag cgttgccgcc gtttgcagac gtggggtgag ccattgttcg tcgtgcgcca
    66001 tctgttactc cttaaacaat gcgaaacgca tcgactaata cacagcgacg cagacggaca
    66061 aacgtccgcg cgctggttac cccttcaccg gttggcgtgg tgatggtcat agtggtccag
    66121 ccttccccgc ccagccccag cccggcaatg cacggtccgt tcttaacgaa aatgctggta
    66181 tcaatggcat tcgccatctg gttcatgttc tcgatattgc gcgagtgcat tgccgccgtg
    66241 tggtggcaac cgccttccag tttcaccgcc agcgcaatgg catccgccac gttggcgacg
    66301 cgcacgacgg gcagcaccgg catcatcagt tcagtcacag caaacggatg ttctgcggtg
    66361 gtttctacaa acagcagtcg cgtttgttcc ggaactttaa ggccgattgc cgccgcgatt
    66421 ttcgccgcat cgcgaccaac ccagtcacgg ctgacggtgc cttttccgcg ctcgtcgata
    66481 tttttcagca acaccgattg cagttgctgc gcctgctctg cggtcagttt caccgcatgc
    66541 tggccttcca tcagacgcat cagttcatcg gctacgctat caacaacaat cagtaccttt
    66601 tcgtcggcac aaatgatgtt gttatcgaaa gaagcacctt tgacgatgga ctgagctgca
    66661 cgggcgaggt cggcggtttc atccaccact accggcgggt taccggcacc tgccgcaatc
    66721 aggcgtttat tggtgtgttt gcgcgccgct tccactaccg cttcgccgcc ggtcaccacc
    66781 agcaggccga tacccggaaa cttgaacaag cgttgcgcgg tttcgatatc cggatttgct
    66841 acggtaacca gtaagttttc cggcccgcct gcggcaacaa tcgcctggtt gagcagcgta
    66901 atcgcccgct gggagacttt tttcgccgcc ggatgcgggg caaaaatgac gctgttgccc
    66961 gcggcaatca ggctgatggc gttattaatg acggttgccg ccgggttagt ggaaggcgtc
    67021 accgaagcca ctacgcccca gggcgcgttt tcaattaaag tcaggccgtt gtcgccagtc
    67081 agcacctgtg gagagaggca ctcaacgcct ggtgtgccgc gcgcctgagc gacgtttttg
    67141 gcaaatttat cttcaacgcg ccccatgccg gtttcactga cggcaagttc cgctaaatct
    67201 ctggcgtgtt tttcgcctgc ttcacgaatg gcagcaatgg ctaactggcg cattgccacg
    67261 ctttttaacc cttgctgggc gactttggct gccgcaacgg cgtcatccag ggacgcgaaa
    67321 acgcccatct catgaacggc ggcgggcggc gtgtcactgc tttgcatttt cagcagtacc
    67381 gctttcacca cctgttcaat atcctgttga ttcatgatgt tctgccttat ttgtggaaaa
    67441 tgacctgacc gccagacacc acctcatcga cgatgccaat cacgcacaga tcgaccggtg
    67501 acgtttcgct tttatgcgcc tggcgggcgg aactgccact caccagcaac acccactccc
    67561 cggttcccgc gccaatattg tcgatggcga cggcgcattg cccgtcagga ttaccttgtg
    67621 gatcaatcat ttccaccatc agcaatttgt catgcgccag tccgtgatgg cgtacggtac
    67681 aaacaatttg tccagtgacg actgccagtt tcatacccgc ctccgtggcg tatttcaggt
    67741 aaaagctccc cctaccctcc gcagaaggta aaatgaaaaa ggagagagcg tgacgcccga
    67801 atcgacgtca cacagggtga ttacaggttg ctgctatcgc ctttcaggcc gatcgggaag
    67861 acttcttcca ggtcgccgtg cggacgcggg ataacgtgta cagataccag ctcgccgata
    67921 cgctgtgccg ctgcggcacc tgcatcggtc gcggctttac aggctgcaac atcaccacga
    67981 accatggcag tacacaggcc gccgccaatc tgcttaacac caaccagctt gacgcgcgca
    68041 gctttaacca tcgcgtcaga agcctcaatc agtgcaacca ggccccgggt ttcgatcatt
    68101 cctaatgctt ccattgtgct ttcctcttta tcagggtcca gaacgggacc gttcattcaa
    68161 ccagtgtttg taaactgctt tcgcggttca cttctgtctg acgctgcaca gctgccacca
    68221 gcgccagttc gataatttcc tgcacgctac aaccacgaga gagatcgtgc atcggcgcgg
    68281 caagtccttg tatcaatggc ccgacggcac gatatccgcc aagtcgttgt gcgattttgt
    68341 agccaatatt tccggcttcc agcgacggaa aaaccatcac attagctttg ccctgtaacg
    68401 ggctggcagg cgctttttgc gccgccactt ccggcacgaa ggcggcgtca aactgcaact
    68461 cgccatcgac aaccagcttt ggtgcgcgct cacggacgat ttctgtcgcc tgctggacgt
    68521 tggcaacgca ggggtgacgg gcactaccgt tgctggaaaa cgacaacatc gccacgcgcg
    68581 gttcttctcc ggtgatggcg cgccaggttt cggcgctggc aagcgcgata tccgccagtt
    68641 gcgccgccgt cggctgtggc actacgctgc aatcggcaaa gcccaatgcg gggccactgt
    68701 actgtggcag catcaggaaa atggaggaga gcgttttaca gcctggctgt aagccgataa
    68761 tgcgtaatcc ggcacgcagc acattcgccg tggaagagag attgcccgcg atacagacat
    68821 ccgctgtccc ggcgctgacc attgcggcag cgaacatcag cggatcggtg agtttatcca
    68881 gcgcatccgg cggcgttttt tcgcccgcac gggccagcca gcggagagca aattcttccc
    68941 gcattgcgag gttgccatgc ggatcaatca cctgtagccc gtccatcgcc acgccgtgac
    69001 tgagcgcaaa ctgacgaagt tcaaacggat tggcgaccag aatgggcgtt gccagacctt
    69061 gttgatgtaa atattgcgcc gctttcagca cacgttgatc taacgcatcc ggaaaaacta
    69121 ctctggcggg cgctcgcaac gccagtttac gacaacgttc aataatcatt gcgtttcccc
    69181 cagtcgttgt tggatctgct tcaccgttag cgactgcttc gttacgctca ggatcatcat
    69241 cacgtaaacc gtgctggaaa ggcggttcag cgcctgcaag atgtccgggc gtaatacttc
    69301 aaagctgcgg gtgataaaca cctgcgccgc tacagtttcg gtttcccgca ctttggtgcg
    69361 tagcagattg agcaacgccg catcacgccc gtggctggct tccggcacca gatgatcgtg
    69421 gtcgagatag cgcagcggct ggtgagaaag ccgatgcaga tcttcgtcag aaagcccgac
    69481 aatcgcttgt tcgcccagcg gttcccccag cgcatcggcg cgcataatgt tgccaagacg
    69541 cgaacggata tccgccagcc acggctgcca cggttccgcc agttcaattt gcagccacac
    69601 tgccagcgca atggtgctgt cgagaacagc gcgaaaagcc aggcgcggat cgcttttggc
    69661 aaccattttt tccgccgaca ggtgagtcag cgtatccggc ttttttgcca ccggctggcg
    69721 gcacagttcg cagcacgcct gcggatgttc atcgctactg gttagcccgt gaacgggctg
    69781 cggctgctgc tgttcatcgt caacaaacag gcggccctgc tcgtcaataa acttgatgcg
    69841 cagatggcgg ctttccagta actcccgggc agagggcgtc aggcgactgt ccgcagggag
    69901 atggatctct gcgccttcgc tgagcgtatg attcgctctt agccatgctt cggtgatgaa
    69961 atctttcata aggattgcca gttagccggc caggcgacat acaggaattt cacgctggat
    70021 gtcgtaccaa attcgatgct ggagcctttc gggataaaca tcacatcgcc cgctttggcg
    70081 atcatagttt cgccttcgtg gcgaacatgc agctcccctt ccagcaccat gtcgatttcg
    70141 tcgtagttca gggtccacgg aaagaaggcg ttctcccact gcatgaaacc agcggccata
    70201 ctgctgccgt cgtctccggt cacgagatca gtcaagccta cgcagtgcgg ttccgcgcca
    70261 tcgaaacgac caaacttgac gctgctgcca tcaataactt tgatgccgcc tttgccggtc
    70321 accgatttaa agctcggctg cagtgcaccc tgctccagcg actgtttttc cttcatcact
    70381 ttttccatca gctgcgcgac caggctttcg gtaaactgac cttccggcag ttgggcgata
    70441 atggtttcac ggatgcgctg gctttcggtt ttatcggctg atgcgctggc gggaacagac
    70501 gccgttaccg ggatcgattc atcacattcg gtgatggtaa aacccaacag atcagccact
    70561 tcacgagctt ccggggtaat aatgctggcg cgcagaacca cggacattgc cagttcgccg
    70621 cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg cttcctcctg
    70681 tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt
    70741 tagctcgaaa atcggctctt cgaagcccgt ctcaagcaat aactttcgcg ttgcggcgac
    70801 gtcagcatct ggcatgtccg ttttactgat gacggcgatt tgccgtttgc tgacgccaat
    70861 atccagcaac ccggcaggca accgactttc cgggtcattc gcgccgtgga catagatcag
    70921 catgtccaca tcctgtagcg tggtaattaa tgcgtgatac cagcggggat ggctaaaata
    70981 ttcacccggc gtatcaatat cgccattatc attaaattcc acggcctgcg tttttctggc
    71041 gagggtataa ttcccctgca acgcattaaa taacgttgtt tttcctgccc cgacggtacc
    71101 cacaaacgca attcgtttca tgaatgcctc atattaactt ttggtcattt cacaaagcgt
    71161 ataatttaat aagcgtccta aaccactgac ggtttgtgat aacgcttctt ctactgcgcc
    71221 caccgaacca taaatcacca gcgcgccgct aaaacggtcg agaaaaccga tatgcacatc
    71281 ggcggctttc aacgctaaat caccggcgat catcgccgtt tctccgggag ttagcgtcat
    71341 aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct cgccgggatg
    71401 cgcgatgaga tgcgccagcg tgacctgttt gccgggcaca aattcctgaa tgatgcgttc
    71461 tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga
    71521 gtaaataaca aaattcggca agcgcgttta aaaggtga
//
LOCUS       DAZKJR010000005        69247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKJR010000005 DAZKJR010000000
VERSION     DAZKJR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.005"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..163
                     /gene="yhfL"
                     /locus_tag="ACHOWS_000331"
     CDS             <1..163
                     /gene="yhfL"
                     /locus_tag="ACHOWS_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFQ0045977.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
     gene            complement(270..1355)
                     /gene="yhfS"
                     /locus_tag="ACHOWS_000332"
     CDS             complement(270..1355)
                     /gene="yhfS"
                     /locus_tag="ACHOWS_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0045978.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVSRIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1367..2671)
                     /gene="yhfT"
                     /locus_tag="ACHOWS_000333"
     CDS             complement(1367..2671)
                     /gene="yhfT"
                     /locus_tag="ACHOWS_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFQ0045979.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2683..3036)
                     /gene="yhfU"
                     /locus_tag="ACHOWS_000334"
     CDS             complement(2683..3036)
                     /gene="yhfU"
                     /locus_tag="ACHOWS_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFQ0045980.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3047..3925)
                     /gene="php"
                     /locus_tag="ACHOWS_000335"
     CDS             complement(3047..3925)
                     /gene="php"
                     /locus_tag="ACHOWS_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFQ0045981.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNIVKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(3922..5148)
                     /gene="yhfW"
                     /locus_tag="ACHOWS_000336"
     CDS             complement(3922..5148)
                     /gene="yhfW"
                     /locus_tag="ACHOWS_000336"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0045982.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALLNADWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITFNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVLTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(5148..6311)
                     /gene="yhfX"
                     /locus_tag="ACHOWS_000337"
     CDS             complement(5148..6311)
                     /gene="yhfX"
                     /locus_tag="ACHOWS_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFQ0045983.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPLAHQ
                     GHLVQIPCHQVSDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVADIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(6395..6757)
                     /gene="yhfY"
                     /locus_tag="ACHOWS_000338"
     CDS             complement(6395..6757)
                     /gene="yhfY"
                     /locus_tag="ACHOWS_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0045984.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVKPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6774..7679)
                     /gene="yhfZ"
                     /locus_tag="ACHOWS_000339"
     CDS             complement(6774..7679)
                     /gene="yhfZ"
                     /locus_tag="ACHOWS_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFQ0045985.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVNRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(7969..8973)
                     /gene="trpS"
                     /locus_tag="ACHOWS_000340"
     CDS             complement(7969..8973)
                     /gene="trpS"
                     /locus_tag="ACHOWS_000340"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0045986.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8966..9724)
                     /gene="gph"
                     /locus_tag="ACHOWS_000341"
     CDS             complement(8966..9724)
                     /gene="gph"
                     /locus_tag="ACHOWS_000341"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFQ0045987.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9717..10394)
                     /gene="rpe"
                     /locus_tag="ACHOWS_000342"
     CDS             complement(9717..10394)
                     /gene="rpe"
                     /locus_tag="ACHOWS_000342"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0045988.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10412..11248)
                     /gene="dam"
                     /locus_tag="ACHOWS_000343"
     CDS             complement(10412..11248)
                     /gene="dam"
                     /locus_tag="ACHOWS_000343"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFQ0045989.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11355..12641)
                     /gene="damX"
                     /locus_tag="ACHOWS_000344"
     CDS             complement(11355..12641)
                     /gene="damX"
                     /locus_tag="ACHOWS_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFQ0045990.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12733..13821)
                     /gene="aroB"
                     /locus_tag="ACHOWS_000345"
     CDS             complement(12733..13821)
                     /gene="aroB"
                     /locus_tag="ACHOWS_000345"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0045991.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13878..14399)
                     /gene="aroK"
                     /locus_tag="ACHOWS_000346"
     CDS             complement(13878..14399)
                     /gene="aroK"
                     /locus_tag="ACHOWS_000346"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFQ0045992.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14800..16038)
                     /gene="hofQ"
                     /locus_tag="ACHOWS_000347"
     CDS             complement(14800..16038)
                     /gene="hofQ"
                     /locus_tag="ACHOWS_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFQ0045993.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15950..16354)
                     /gene="hofP"
                     /locus_tag="ACHOWS_000348"
     CDS             complement(15950..16354)
                     /gene="hofP"
                     /locus_tag="ACHOWS_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFQ0045994.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            complement(16344..16784)
                     /gene="hofO"
                     /locus_tag="ACHOWS_000349"
     CDS             complement(16344..16784)
                     /gene="hofO"
                     /locus_tag="ACHOWS_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFQ0045995.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16768..17307)
                     /gene="hofN"
                     /locus_tag="ACHOWS_000350"
     CDS             complement(16768..17307)
                     /gene="hofN"
                     /locus_tag="ACHOWS_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFQ0045996.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(17307..18076)
                     /gene="hofM"
                     /locus_tag="ACHOWS_000351"
                     /pseudo
     CDS             complement(17307..18076)
                     /gene="hofM"
                     /locus_tag="ACHOWS_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            18196..20748
                     /gene="mrcA"
                     /locus_tag="ACHOWS_000352"
     CDS             18196..20748
                     /gene="mrcA"
                     /locus_tag="ACHOWS_000352"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFQ0045997.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20916..21476)
                     /gene="nudE"
                     /locus_tag="ACHOWS_000353"
     CDS             complement(20916..21476)
                     /gene="nudE"
                     /locus_tag="ACHOWS_000353"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFQ0045998.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            21796..23931
                     /gene="igaA"
                     /locus_tag="ACHOWS_000354"
     CDS             21796..23931
                     /gene="igaA"
                     /locus_tag="ACHOWS_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFQ0045999.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAHDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23996..24664
                     /gene="yrfG"
                     /locus_tag="ACHOWS_000355"
     CDS             23996..24664
                     /gene="yrfG"
                     /locus_tag="ACHOWS_000355"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFQ0046000.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24675..25076
                     /gene="hslR"
                     /locus_tag="ACHOWS_000356"
     CDS             24675..25076
                     /gene="hslR"
                     /locus_tag="ACHOWS_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFQ0046001.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            25101..25979
                     /gene="hslO"
                     /locus_tag="ACHOWS_000357"
     CDS             25101..25979
                     /gene="hslO"
                     /locus_tag="ACHOWS_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0046002.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(26117..27841)
                     /gene="yhgE"
                     /locus_tag="ACHOWS_000358"
     CDS             complement(26117..27841)
                     /gene="yhgE"
                     /locus_tag="ACHOWS_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFQ0046003.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRRRGLLMAFDGVQRLQQQISEKA
                     LADNVFIASGSGKPDTIFWSAVMKNRYNAMICIEKDACVLVEQDLNSDGRAERILFAF
                     NDERYIIYGIDPAGKEWKEITMNSLPAVITKEKLLMAAEDGKLGTKPKVWRDLTVDGE
                     RLDLNLNE"
     gene            28220..29842
                     /gene="pckA"
                     /locus_tag="ACHOWS_000359"
     CDS             28220..29842
                     /gene="pckA"
                     /locus_tag="ACHOWS_000359"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0046004.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            29957..30274
                     /locus_tag="ACHOWS_000360"
     CDS             29957..30274
                     /locus_tag="ACHOWS_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0046005.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            30333..30629
                     /gene="nadS"
                     /locus_tag="ACHOWS_000361"
     CDS             30333..30629
                     /gene="nadS"
                     /locus_tag="ACHOWS_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFQ0046006.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(30661..32013)
                     /gene="envZ"
                     /locus_tag="ACHOWS_000362"
     CDS             complement(30661..32013)
                     /gene="envZ"
                     /locus_tag="ACHOWS_000362"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFQ0046007.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(32010..32729)
                     /gene="ompR"
                     /locus_tag="ACHOWS_000363"
     CDS             complement(32010..32729)
                     /gene="ompR"
                     /locus_tag="ACHOWS_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFQ0046008.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32957..33433
                     /gene="greB"
                     /locus_tag="ACHOWS_000364"
     CDS             32957..33433
                     /gene="greB"
                     /locus_tag="ACHOWS_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFQ0046009.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            33530..35851
                     /gene="yhgF"
                     /locus_tag="ACHOWS_000365"
     CDS             33530..35851
                     /gene="yhgF"
                     /locus_tag="ACHOWS_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0046010.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            36289..36516
                     /gene="feoA"
                     /locus_tag="ACHOWS_000366"
     CDS             36289..36516
                     /gene="feoA"
                     /locus_tag="ACHOWS_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFQ0046011.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            36533..38854
                     /gene="feoB"
                     /locus_tag="ACHOWS_000367"
     CDS             36533..38854
                     /gene="feoB"
                     /locus_tag="ACHOWS_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFQ0046012.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            38854..39090
                     /gene="feoC"
                     /locus_tag="ACHOWS_000368"
     CDS             38854..39090
                     /gene="feoC"
                     /locus_tag="ACHOWS_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFQ0046013.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            39293..40174
                     /gene="rpnA"
                     /locus_tag="ACHOWS_000369"
     CDS             39293..40174
                     /gene="rpnA"
                     /locus_tag="ACHOWS_000369"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFQ0046014.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGFQKGIRAIAKTMLDAGVPLEDV
                     LRFTSLTVEDLAEINHQ"
     gene            40246..40365
                     /locus_tag="ACHOWS_000370"
                     /pseudo
     CDS             40246..40365
                     /locus_tag="ACHOWS_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(40394..41164)
                     /gene="bioH"
                     /locus_tag="ACHOWS_000371"
     CDS             complement(40394..41164)
                     /gene="bioH"
                     /locus_tag="ACHOWS_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFQ0046015.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKMVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            41202..41796
                     /gene="gntX"
                     /locus_tag="ACHOWS_000372"
                     /pseudo
     CDS             41202..41796
                     /gene="gntX"
                     /locus_tag="ACHOWS_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
     gene            41855..42430
                     /gene="nfuA"
                     /locus_tag="ACHOWS_000373"
     CDS             41855..42430
                     /gene="nfuA"
                     /locus_tag="ACHOWS_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFQ0046016.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42791..44107
                     /gene="gntT"
                     /locus_tag="ACHOWS_000374"
     CDS             42791..44107
                     /gene="gntT"
                     /locus_tag="ACHOWS_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFQ0046017.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(44152..46236)
                     /gene="malQ"
                     /locus_tag="ACHOWS_000375"
     CDS             complement(44152..46236)
                     /gene="malQ"
                     /locus_tag="ACHOWS_000375"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFQ0046018.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTFTQEDQRVHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQTRDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QVAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGSETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(46246..48639)
                     /gene="malP"
                     /locus_tag="ACHOWS_000376"
     CDS             complement(46246..48639)
                     /gene="malP"
                     /locus_tag="ACHOWS_000376"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFQ0046019.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELAEYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWTNDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49261..51966
                     /gene="malT"
                     /locus_tag="ACHOWS_000377"
     CDS             49261..51966
                     /gene="malT"
                     /locus_tag="ACHOWS_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFQ0046020.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGDWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWHNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(52009..53025)
                     /gene="rtcA"
                     /locus_tag="ACHOWS_000378"
     CDS             complement(52009..53025)
                     /gene="rtcA"
                     /locus_tag="ACHOWS_000378"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFQ0046021.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(53029..54255)
                     /gene="rtcB"
                     /locus_tag="ACHOWS_000379"
     CDS             complement(53029..54255)
                     /gene="rtcB"
                     /locus_tag="ACHOWS_000379"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFQ0046022.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            54444..56042
                     /gene="rtcR"
                     /locus_tag="ACHOWS_000380"
     CDS             54444..56042
                     /gene="rtcR"
                     /locus_tag="ACHOWS_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFQ0046023.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRL
                     ELLHDARSRSLFETLKCDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLSQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITREVVEDEINRLRYNWQES
                     RPSAITALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(56024..56782)
                     /locus_tag="ACHOWS_000381"
     CDS             complement(56024..56782)
                     /locus_tag="ACHOWS_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFQ0046024.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(56799..57629)
                     /gene="glpG"
                     /locus_tag="ACHOWS_000382"
     CDS             complement(56799..57629)
                     /gene="glpG"
                     /locus_tag="ACHOWS_000382"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFQ0046025.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(57674..58000)
                     /gene="glpE"
                     /locus_tag="ACHOWS_000383"
     CDS             complement(57674..58000)
                     /gene="glpE"
                     /locus_tag="ACHOWS_000383"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFQ0046026.1"
                     /translation="MEQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWRRQFPA
                     EVAYGA"
     gene            58190..59695
                     /gene="glpD"
                     /locus_tag="ACHOWS_000384"
     CDS             58190..59695
                     /gene="glpD"
                     /locus_tag="ACHOWS_000384"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFQ0046027.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(59749..60354)
                     /locus_tag="ACHOWS_000385"
     CDS             complement(59749..60354)
                     /locus_tag="ACHOWS_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046028.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRTPPELKNRLHYWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(60357..61133)
                     /locus_tag="ACHOWS_000386"
     CDS             complement(60357..61133)
                     /locus_tag="ACHOWS_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046029.1"
                     /translation="MQPSSAMQALIEQVYHIFRRYPVPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDERAILQRFACQYMADWVSGDEAVELQYMLEMFSRADIALSPLLDAIIS
                     VPGYQPTVSMACLLWHNREENIQNSRFEQDDDDKAITAQINAWAFNNQFILKERARQA
                     IENLLNQPEQGTHYQVWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(61136..61846)
                     /locus_tag="ACHOWS_000387"
     CDS             complement(61136..61846)
                     /locus_tag="ACHOWS_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046030.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIIQQLINQV
                     LDEGEKFAGTVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAELKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            complement(62020..63141)
                     /locus_tag="ACHOWS_000388"
     CDS             complement(62020..63141)
                     /locus_tag="ACHOWS_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0046031.1"
                     /translation="MITYAGYKVKIIFIIFLFLGYHVVAYAEMGRDREFCYPGTPGNN
                     TTPATFFYDFGTVIISDVNNNSAGTVLPSQNWKIGRYKAYCNSLDDYEVYFSGISGID
                     PSGASGNPQGAEIFIPVTHEISISTHIKLYNQNGTMTDKTVPFENYSTNYPADRSKAD
                     YWSSGTEGYIKIRLDKKIISDVSLNNVLLVSLYASQRQTEHGPVPLFNAWISNLDIQV
                     PQGCTINEGTSFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDTDAVMTL
                     VFDGNISATRDTNGKQSIIQAQDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLVENQ
                     AASAQTADVTFLALPVSTTGKVPAAGRYNALAVLRVEYQ"
     gene            complement(63156..63935)
                     /locus_tag="ACHOWS_000389"
     CDS             complement(63156..63935)
                     /locus_tag="ACHOWS_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFQ0046032.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNISYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCK
                     AQGICEITWQTLDIYNNVLPVWKVNMNPLQMDVSGSQFKTD"
     gene            complement(63901..64473)
                     /locus_tag="ACHOWS_000390"
     CDS             complement(63901..64473)
                     /locus_tag="ACHOWS_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0046033.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTNSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEDGSVDGLALTIFDKNKGSISNDVKSIGFSLTSSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFIVTYD"
     gene            complement(64424..64987)
                     /locus_tag="ACHOWS_000391"
     CDS             complement(64424..64987)
                     /locus_tag="ACHOWS_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0046034.1"
                     /translation="MNLIFSKYSVFFILFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRLSAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(64987..67581)
                     /locus_tag="ACHOWS_000392"
     CDS             complement(64987..67581)
                     /locus_tag="ACHOWS_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFQ0046035.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALALSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELNNPV
                     SYATLECR"
     gene            complement(67618..68370)
                     /locus_tag="ACHOWS_000393"
     CDS             complement(67618..68370)
                     /locus_tag="ACHOWS_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFQ0046036.1"
                     /translation="MKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNTFVPPKSDI
                     TVSSAVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(68471..69175)
                     /locus_tag="ACHOWS_000394"
     CDS             complement(68471..69175)
                     /locus_tag="ACHOWS_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0046037.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDIEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT        17157 a        18499 c        16840 g        16751 t
ORIGIN      
        1 caaatttatt aaagttgcac tggtaggtgc ggtactggct acgttaactg catgtactgg
       61 tcatattgaa aaccgtgata agaactgctc ttacgactac ctgctgcacc cggcaatttc
      121 tatttctaaa atcattggcg gttgcggtcc tactgcacag taagtctctg actgaaagcg
      181 gctaacaccg gtttaattgc ctgacgccct cggggttacc cgagggcgta tttatttctg
      241 cattcttttc gaaaacatca tgcacaagat taaatactgg caatactctc acgcaaaatc
      301 cgcagcaccg tctcttcacc gctgcgattt gggttaatac gaatcgcgta atgttcgagt
      361 tgcggattcg cctggcgaaa cgttccggaa agtcgataaa aaagtggcgg aatttcatat
      421 ttcgactctg caccaaccgg gtaaggcaag gcaccgcgct tttgcgcctc ttccagtact
      481 ctggcggcaa tcggctgatg aaactccaca atcaacacct tcgactgcgc attggcaatc
      541 accgcgcttt tcacttccgc cactgcgcca ccgttaagta aagccagcaa ccgttcagat
      601 acccccgcct gcaccgcgtg catcactggc gcgagcacca gaccacgcaa cacctctaat
      661 gcctgcgccc cctgaatctg actgccgccg gaatagagcg tggcgcgaat ccggctaata
      721 acatcggcat cgcctaccac cgcaccaacg ccctctggcc caaatagctt gaagcaggaa
      781 aatgtcgaca cattcgcccc gcattcgcag ccaattcgcg acaccttcat caccgcatag
      841 ttgtcatcgg ttaacgctgg aacacctgcc gcgcgcaacg ttgccagcac gtctgccagc
      901 acatagcggt cctgcggctg ctggcgcgta tgctgcacca gcgcggcatc aggttgttgc
      961 tcgtcaacaa cctgcttcag tgccgacaga tcattgaaat cagccgtaat gagcgttagc
     1021 cccatctgct caataatgac gcgcgtcgtt ggataaaccg gcgcatcatg caccagaaga
     1081 cgttgccccg gtttgagcaa agccgccagt gccgcacgaa tcgcgccagt acccgcgccc
     1141 tgtaccagtg ccgccgcggg tgcatgaaac gcgtcagcca gcacctgttc cacacgctgg
     1201 gtaatgcggg gctgatttag ccccggcgtt aagcccaaat caccgcaggt aagaaactcg
     1261 ctgccaggaa aatgacggca aatcgtgtcc accagcgcaa actgcttttg ctgcgcctca
     1321 ataagcgtca ggctttgcag aggaaacgtc ttcataggtg ccttccttaa gccggaacga
     1381 acaggccaag ccagtaaaga acattcagca ggatgccggt gatcattact gctacgaccg
     1441 gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa atggcaaccg
     1501 cgatagagaa tccggtatag cccgccattt tgattgctgc aaagatcgaa ccgaccagca
     1561 acgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca ttacgtaccg
     1621 acgggtagcg ccccaaccat ttgccgatcg aacgaagcag caagacttcc gccgaaataa
     1681 ccactgcgcc taataccgct gcaaccatcg gattcggcga gagatagccc accgcataaa
     1741 caaaggtaaa gcccgcaact gcatacacac cagtggctaa cgcggtggtg gcaatcaacg
     1801 gtacaaaacc cagcccgcgc ataaattccg ccagagctgc ctggttgatt agtgtttgcg
     1861 attgttccgg cgttacgcct gcggaatatg ctttctccag tgtgaagatc gacacttcac
     1921 tgccagcaaa aatcttcatg ctggcaacgg cggcaatcag cgccccgaca atagcgatat
     1981 acggtaagtt tttgataatc cgtgacgtgc gttcttcaaa caccgaaagc ccgctggcat
     2041 caatgtcatt ttcatcacga tgacgcaggt cgtgagtaat cgcgatcccc agcagcatca
     2101 ccatgccaat aaagatttcg atggattccg gattgagatg cgggaaatag cgcactacta
     2161 ccacacgggt catcagtacc accacggcgg cgatcagact ttgcttccag ccaaactggt
     2221 agaaaatcgc caccaacggg aacagtgcaa aagctgaaac caccggcgag cttaattccc
     2281 ccaggctacc taatacatcc accggtagcg cggtcagcag ctggtttacc ggtaacaggc
     2341 aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg ctgtttatcg
     2401 ccagtacgcc gaggatatcg gtaggaagaa agagtaacca tgcgttgagc agtccggttt
     2461 tcagggtaaa agagatcccc accgaagcca caaaaccaat gctcagacca aaagcgatac
     2521 tccccgcctc gcgacggttc atatagcctt caatcagttg cggcaggatc gggcggatac
     2581 cgtcatgaaa aacggccgcc gagcgatgcg ccagaagcga tgtcataccc gtcaggcacg
     2641 ccaccacgat aatctgaata tacagatcca tagtcgtgcc ttttatttca gatggttaat
     2701 cagcatcgga atcgcgtgtt cgacgtgctc aacggaaagc ccaaacgcca ctttcccttc
     2761 ggcgatcatt ttggcgatat gttcgtcttt cgctttaatg cctggtttgg caatggtgca
     2821 acttttgtta tagccaatca ccgcgatggc aatcgacaat gccgcgcccg caccggtatt
     2881 acacgcgccg atgtagtaat ccagttgccc ggatttgact ttcattgccg cttccatgtc
     2941 gttgtgaatg aaaacttcaa aacagccagg agccgtcgct tcaatggttt ttttaatctg
     3001 ctcacgctgt aagcctgcaa caccaatctt tttcatgagt ctgtccttat tggaaaaatt
     3061 gagagggagt ttcacgtaac atcacatcca catcggcctg actgaatcct gactggcgca
     3121 attgcggaat aaaggtggtc aataaatagt catagccata accaccgttg gcttttaaat
     3181 gggagcggcg cgtaatatcc atcgacagca tgacgcggtt aagcaaacct cggtcacgta
     3241 gcgcgtggag catcgcaatg cgcttttcgt ccgggtagta actgttctta ccgatggtgt
     3301 cgaactgcac atacgcgccg agatcgatca tcttcacaat gttgtcgagg ttgtctttca
     3361 gatcgcagtg accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc agtagcgcca
     3421 gttgttccag ccccatggtg ctaaaagagg tatgcgtgga gatcgggcgt ccggtctggt
     3481 tatgcgccag cgcggcggca ataaatacct tctcttccag cggcgtgatt tttccttcgc
     3541 tggagcctat ctccgcgatg atcccggctt tcagctccgt gccatcgata ccctgttcaa
     3601 tttcatcgac catctcttgc gccagttcct gcacgctgcg ggtcgccaca tgttccggga
     3661 aaaacgcgtc ctggtaataa ccggtacagg ccaccacgtt gatcccggtt tcgcgcatta
     3721 catcgagcat aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca atcacattac
     3781 gcacgccctg ggccatcagg tcgttcatct cctggcaaat gaacgcatac tgatcaaggc
     3841 ggcagtccac gttgttttta aagccggaga gatcaatatg cagatgctca tgggcgaggg
     3901 tgtagcccgt cggatcaaaa ctcatagggt gtctcctgca aaccggaggg aggaaagaaa
     3961 agagcgacca ttttgtggcg gtggcgcgcg gaaaaattca catacggtag cccccacatc
     4021 agaaagcgtg gtgagcacac caagctgcgt agcgaccatc ccttgctgat aaaccagcac
     4081 tggcaccact tcgcgggtat ggtggctgtg accaatggtc ggatcgttac cgtgatccgc
     4141 catcacgacc aggcaatcat ctggctgcat cgcctcaata agccgggcaa ggttacggtc
     4201 aacgacctgc aaacgttccg cataacgtgc gacgtcttct gcatgaccag cgaggtcggt
     4261 ttcctggata ttggtacaaa taaacgccgt cggatgggtg ttaaattcgt tgaaggtgat
     4321 atccataatc cgctggctat ccaccaggtt ttgccagctc acgccataag gattactggc
     4381 aatatccgcc actttcccca ccagcacggt tggtacgccc acttcataca gtttttgtgg
     4441 cacctgcact ttttcatcga cgccgtagcc catatgcacg acctggaagc cgttgtcata
     4501 agcgccagaa cgcggcgcat tgatgccaat aaagcgccct tctttgcttt ctgcggcatc
     4561 gaggatgcgc tggctgtcgg ttaacaggcc accaaatgta atgacccgac cgacctgtac
     4621 ctgctcacgc acgatacgac caattttgat tgcgtcgtca aaagagatca cagagagatt
     4681 ggcggtgatg ttatagacct gacctaaatc cgcctcgaga ttatcgccaa tcgcaaccgc
     4741 ctgattgacc cacagaaatt gcagatcatc gccacggcgc tccacctgcc agtcagcgtt
     4801 aagtaatgct tgctcaacac ggtcaatcac atcgcgaaag ggcatccgta gcggcggtaa
     4861 cgggcgcgtg cctaaaattt cctgatgccc cataaaggta tcgccacctt catgttgcag
     4921 ctctgccacg ccccaggttg cggagtctga cggctgcata tctccaggcg cataacctaa
     4981 tgcgttgatt agccccattt tctccagcat tggtagctgc aaatgcggca actggctcag
     5041 gatgtgacca caggtatttg ccccggcatc ctgcggacgc accaacgtga catctttcat
     5101 tgcccctacg ccaaagctat caatcactaa caccacaaat cgcgccatta cgcccccaga
     5161 gagtttccaa gactgtcata acgaccgacg atttctggtt cgccacggtg aataccggac
     5221 agcaacacca catcgctacg ggtgacaaaa atctgcgtgc gaaaacagag cactactgca
     5281 ctgcttaccg gaaactcgcc tgccagcggc agggtgtagt cgatactgct gtcatccaca
     5341 gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccag cgcatgttgc
     5401 gcgtgaccac gacggtagta accgccgccg tagcagtagc tgtcgccacg aaaatgatgg
     5461 gagatttcac ttaaccagag catcgcgata cgttcaggct gatcgccctg ctggtttgcc
     5521 ggaatcgtcc ccgtcagcgc atgaccgggt tcggcatgag tcacaccgta ttccgccagc
     5581 aatggcagcg aagtgcagct ggtcgcagaa ggtgcgttca gttgctcaat cgcaatacca
     5641 gatttcgcca gttgatcccg tgcctgtatc agcgtgtgaa gattcggtgt cggcaaaact
     5701 ttcccggcag cctcatccca aagcaggcaa gggaaatggg ttagtccggc taaatgcagc
     5761 cccggcagtt tcttaatatc agcaaccacc tcgtgcagcg aatgctgtac aaaaccgctc
     5821 tcctgccccg gataaagaaa atcatcgtcg ctataaactt tgagcagcac agactgcaca
     5881 cgtccggcct tcaccgctgc tgcagaaact tcccgcgctt tatcgagagt aaacacggtg
     5941 atgacgtcag ttccctgtgc aacggcgtca gaaacctggt gacaagggat ttgcaccaga
     6001 tgcccctgat gcgccaacgg cagaccggcg cggcgcatga cccgcgcctc tttgtaatcc
     6061 accgccacaa tgccgctgta gcccagtgcc agaagttttt ccgccagcca cggattacgg
     6121 ccaaactgct tggtcatcag atacagttca atgccgtaaa gtcgcgccgt ctcaatcagc
     6181 cgcttaccgt tttccagtat ctgatccaca tcgatcaccc agctgtccgg ggcgatcttg
     6241 ccctgctgcc acaaacttag tgcggcggaa atcagcgccg ggttctggcg tttcaatgct
     6301 tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat ttttctgaat
     6361 atttagatta tgcgacgtgg atttgtcggg agcttcaaac ctcttcgtta gcagccatcc
     6421 ataatccata aagattagcc agcaaatagc cttcttcaca cggcttaact tccagtgcga
     6481 attccttcaa caacacctga tgcaattgca caacggcttg ccagtggctg gattgcgcca
     6541 gctctgccag caattcgtca tccagcggct ctatttcttc accacggcga ctgcgcatca
     6601 gtgcgctcgc catatgtgtc atcgccatca ttccttgctc gctgcgcacc ggtagatgac
     6661 actctgtttc cagtacgttg acgacctgca tcatgccttt gcagatgtcc ttatcaataa
     6721 cgcctgcctc acaaagcagg ttgagtctgg tttccatttc agatgccttt cgtttaataa
     6781 ctcggttcct gttccccact gaccactcgt tgttgatggg caagcagggc ctgtttatca
     6841 acaactgcac gcagcaattg ttgcattggg taatcatcga ctcgcgtcaa aactacggct
     6901 tcggtggcct gtaaaaatcg cggatcgtct gtgagcggcg tcgcttctaa tcccagcatg
     6961 gtcagttcgt tttccgccac cacgttccag atcaccgcat caacatcgcc tttaacaatg
     7021 cgttgtaaac tctcgtgata agagagatcg actcgttcca catcactatc gccaaaaaag
     7081 acatcggtca tgattttctg atccgccgaa cggttatcca gccccacgcg attcacgttt
     7141 gcggactcgc ctttacggca aatcaactgg tgctcgccaa cgtaggtgtg cggccccaac
     7201 tccagcgcga ggcataaccc tttttgcgtg agataacttt ccgccgccag tcgggaaaca
     7261 accgccatgt catacacgcc gttaagtaaa cactccacgc gaatatccgc gccgcgcatg
     7321 tgcgcatagt aaaaaggaat accatcaaac tgagctttca atccgctcgc caggccttcg
     7381 tacaaacggg tatagggcaa gggcatcgcg cacaccacat tgttgatatc cacatgactc
     7441 agcaatgctt tgttatccat ttccaccaga taactgccat tacgcccacg gcgttctatc
     7501 cgtatcgctc cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc aacggatgaa
     7561 cggcattcag ttgccagttc atctatggtt ttcagtcgat taccgcactt ttcacccaac
     7621 aaataacggg ccagtgtcgt gatgacgacg ccttcttttt tgataaacgt tcgacgcata
     7681 ataaattttc agtaaattga atatttatat cttcaggaat ttgaagataa ggcgcaacag
     7741 cgagatgtgg aaacggcgag gcacttcaca ttttttcgga ttattgcaaa tgaggataaa
     7801 aaaaccgggt ttccccggtt tcagagtgat gataaaagca aaattgcctg atgcgctacg
     7861 cttatcaggc ctacatttcc ttgcaatatg tgcattactt tgtaggccgg ataaggcgtt
     7921 cacgccgcat ccggcatgaa caaagcgcaa tttgccagca atagtgaatt acggcttcgc
     7981 cacaaaacca atcgcttcgt acaccgcttt cagcgtacga gaagcgtgtg cactggcttt
     8041 ttccgcgcca tctttcatca cctgttgcag gaaggcttcg tcattgcgga aacggtgata
     8101 gcgttcctgc aattcagtca gcataccgga aacggcatca gccacttcgc ctttcagatg
     8161 accatacatc ttgccttcga actgtttttc cagttccggg atgctctggc ccgttaccgc
     8221 agaaaggata tccagcaggt tggaaacgcc cgctttgttc tgcacatcgt agcgaactac
     8281 cggcggctcg tcggagtcag tgaccgcacg tttgattttc ttcactaccg atttcggatc
     8341 ttccagcagg ccgataacgt tattgcggtt atcgtcagac ttggacatct ttttggtcgg
     8401 ctccagcagc gacattacgc gcgcgccaga tttcggaata aacggctccg gcactttaaa
     8461 gatctcgcca tacagcgcgt tgaaacgctg ggcgatatcg cggctcagtt ccaggtgctg
     8521 tttctggtct tcaccgaccg gtaccagatt agtttgatac agcaggatgt ccgcagccat
     8581 cagcaccgga tagtcaaaca gaccagcatt gatgttctcg gcataacgcg cagatttatc
     8641 tttaaactgc gtcatgcggc tcagttcgcc gaagtaggta tagcagttca gtgcccagcc
     8701 taactgtgca tgttccggca cgtgggactg aacaaaaatg gtgcttttct caggatcgat
     8761 accacaagcc agatacaagg ccagcgtatc cagcgtcgct ttacgcagct tctgtgcatc
     8821 ctggcgcacg gtgatcgcgt gttggtcaac gatacagtaa atgcaatgat agtcatcctg
     8881 catgtttacc cactgacgca gcgcacccat gtagttacca atggtcaatt cacctgaggg
     8941 ctgtgcgcca ctaaaaacga tgggcttagt catttttcga ttcctgattt tcgctatgcg
     9001 gaagccctaa tgcgggcaga aggtcattta tagactgata aattacatca ggctggctga
     9061 gatcgatagc ctcgccgtag ttatatccgt aggttaagcc aactgatggg caacctgccg
     9121 cttttgctgc ctgaatatca ttgcgtgagt cgccaacaaa cagcatctgt tgtggggcaa
     9181 ttcccatccg ctcagccacc agtaacagcg ggtccggatg cggttttttg ttttgcacat
     9241 catcaccgcc gataaccacg ctgaagtatt tggcgatatc taaggcttcg agcagcggcg
     9301 cgacgaacgg cgtcggtttg ttggtgacca ggcctagcgg caggccttta gcctgcaatg
     9361 cgcccagcgt atcggcaacg tgcgggaaca aaaacgtccc ctcttcggca acctcgccat
     9421 agtagcgatc gaacagttta cgcagaatac gtacctgttc ttctgccgga atgtcgtcat
     9481 caacgggcgg tttacccatt gttttacgca gagtcgcacg ttcctgacgc gcccaggtca
     9541 atgcgcgctc catcagaata tctgcgccgt taccaatcca ggtaataacg cgttcttcac
     9601 ctgcgacagg caactccagc gcatacagcg ccatatctac cgcagcagca agaccgggtg
     9661 cactgtcaac cagcgtaccg tcgagatcaa aagcgacgcc gcgaatatct tcaaacttat
     9721 tcatgactta cctttgccag ttcactgcgc atttcatcaa tgactttttt gtagtctggc
     9781 tggtcgaaga ttgccgaacc agcaacaaac atatccgcgc ccgccgcagc gatttcgcca
     9841 atgttgttca ccttcacgcc accgtccact tccaatcgaa tgtcaaagcc agactcgtcg
     9901 atacgacgac gtacttcgcg cagtttatcc agtgtttgag gaatgaaaga ctgaccgccg
     9961 aaaccaggat tgacggacat cagcaggatc acatccagct tatccatcac gtaatccaga
    10021 taactcagag gtgtcgccgg gttaaatacc agacccgctt tacagccatt ttctttaatc
    10081 agttgcagcg tgcggtcaac atgctcggag gcttctggat gaaaggtaat gatgctggca
    10141 ccagcggcag cgaaatcagg cacaatgcga tcgacgggtt tcaccatcag gtgtacgtcg
    10201 ataggggcgg taatgccgta gttacgcaag gatttcagca ccattggccc aatagtcaga
    10261 ttgggaacat agtggttatc catgacgtca aaatgcacga catcagcgcc agctgccagg
    10321 gcttttgcgg tatcttcacc caggcgggca aaatcagccg acagaattga gggggcaatc
    10381 aaatactgtt tcatccgctt ctccttgaga attatttttt cgcgggtgaa acgactcctg
    10441 gtttgtacaa agccagcagt tcgtccacct ttttacgtgt gccgccgttg ctgcttatac
    10501 tgcgtcgaac tttgacgaca tgcaattttg cgcgctgata ccactcacgc gttaacatcg
    10561 tatcgtgatt ggagatcagc actggaatat ggcgatcaac cagaccttcg gcgatctccg
    10621 ccagatgcgc ttgttgttca agcgtaaaac tgtttgtgtg atacgccgta aagttggcgg
    10681 tcgcagacag cggcgcataa ggcggatcgc aatagacgac ggatgcatca tctgcgcgcg
    10741 ccatgctatc ggcgtaagac tcacagtaga aaaaggcatt ctgcgctttt tcagcgaagt
    10801 gatacaactc tgcttccggg aaatagggtt ttttgtagcg gccaaacggc acgttaaact
    10861 caccgcgcag attgtaacga cacagaccgt tgtaaccgta gcggttcaaa tataaaaaca
    10921 gtaccgcccg acggaacgga tcctggcttt tgttgaactc ttcgcggaac tgatagtaaa
    10981 cctcggcgca atttgtttcg ggaacaaaca gctcgcgtgc ggcctgtacg tactcatcag
    11041 tacgcatctt cacaatgtta tagagactga tcaggtcgct attgatatcg gcaaggatat
    11101 aacgagaaaa gtcggtgttg agaaacaccg acccggcacc tacaaaaggc tcaaccagac
    11161 attcgccctt gggcaaatgc cgtttaatat catcaagcag gggatacttg ccccctgccc
    11221 acttcaaaaa agcgcgattt ttcttcatgc tgactaacta attacacctt ctccggctgt
    11281 ggagaaagct ccgacagcat ccagcgcttc aggcaactcc cgcgatgata aaccattgcg
    11341 ggagataacc ttgattactt cagatcggcc tgtacctgac gcagcggttt cgcccacggg
    11401 tttttggcct ggacatcggc tggcaatgta gataccgctt ttttcgcctc ttctttcgaa
    11461 gcgtacacgc cagaaaccag gacataccac ggctgaccat tacgcgtcgt ttcatagaca
    11521 acgtagtttt tcagattctc tttcttcgcc caaccgttca ggttgtcgta gttagaggaa
    11581 ctgctcagct gcagagtgta atggctggac ggtgccgatt tcaacgaacc aacattacct
    11641 gcggtcttcc ctccagcggc tggtgttgcc gtggtttgcg ccggggatgc cgtctgtact
    11701 ggagccgtag tcgccgtctc ttttggtgct ggcgtagaag tcgcagccgg tgccttcgtg
    11761 cttgccacag gagcagctgg ttcagtacgc ttcggcgtct gtgctaccgg cttcggctcc
    11821 gttttcacgg ttgcttgcgg ttttttcggt tcaatcaccg cctgctggcg agctggacgc
    11881 gtagtggccg gacgttcagc ggtttgcgtt ttcgccgtgt cacgcgatgc attgccattg
    11941 cgaacaggcg cgaccgtcgc gggttcagtc ggcaacgtgg aattgaccgc cacattattc
    12001 aactgttgct gattttgtgg ctgggtcagc gcattgttca ggtcaccctg cacttcaaca
    12061 cgttgttgac catccgttgc cgccggggtt tgcccttgag tcggcgtaga agagatcggc
    12121 ggcagagaaa catcctgctg agtattttcc gcagacgtgg ttcccggcgc tggctgcacg
    12181 ccattcgcct gatcggtcgc attgccagca agatcaatac tcttctcgcc agacgcggtt
    12241 tgatcgctgg aagaggtcga gggggctttt agcgcagaac cgataccgat gatcaacagc
    12301 aggagaacca gaatgccgac gcccatcatc atatactgac gagaagcggg tttactggct
    12361 gcttttttgc gcttacgtgg acgacgctct acgcgctctt catccacggt ttcatcttcg
    12421 gattcgtcaa tttcttcttc gagttccggt tcctcattgc gctctttttg cgcacgagtc
    12481 ggacggcgat cgtcagtgtc atcaagttca atatcatcaa aattgatctg cggttcgcca
    12541 cgttcagtac gctcagaacg ttcagaagat tgacgagaac gaccagtacg acgatcgctg
    12601 ggatcgggtt tcagctcgtc ttctggtttg aattcatcca tttaacaccc cactaaaagg
    12661 ttaatgctta ccacgttgca attaacctga agctaataga ccgcttgata agcggcctga
    12721 cctttcttgt tgttacgctg attgacaatc ggcaatggcg ttaagaacaa gctcgtgcga
    12781 aacgccgctg cgaacttcac tcttaccaat tgccaacgga agaattaagc gcatctctcc
    12841 cgcaaggact ttcttgtcac gcagcatatg cggtaaatac gcctgcgcag acatttcgcg
    12901 cggcccattg accggtaacc cagcccgcgt gagcagggtt ataatacgct gcgtttcggc
    12961 agaactaaac tgcccgagac gttccgacgt ccgcgccgcc atcaccatac ccgcagcaac
    13021 cgcttcaccg tgtaaccaat tgccataccc catttcagct tcaatggcat gaccaaaggt
    13081 gtgtcccaga ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg cgacaacttc
    13141 tgccttcagt tcacaacaac ggcgaataca gtacgccatt gccggaccgt ccagacgcaa
    13201 caacgcatcc agattctctt ccagccagtt gaaaaacgca ccgtcaagaa taatgccgta
    13261 tttgatgact tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt tcagacagtc
    13321 gagatccacc accactgaag caggctggta gaacgcgcca atcatgtttt taccgagggg
    13381 atggttgacc gcagttttgc cgccaacgga ggaatcgacc tgcgacagca atgtcgtcgg
    13441 gacttgaatg aaacgtacac cgcgctgata actcgccgcc gcaaagccgg tcagatcgcc
    13501 cactacgccg ccgccaagcg ccaccagcgt agtatcgcga ccatgcggct tttgtaacaa
    13561 cgccgtaaag acggtatcga gtacagccag gcttttatac tgctcgccgt cagggaggat
    13621 aacgctatcg acgttaacac ccgcctgttc aagtacgccg cggaccttat cgagatacag
    13681 gggagccagg gtttcgttgg taaccaacat gacctgctcg cccgatttca gcggtaagaa
    13741 tgaagctggt tcattaaaca aaccagatgc gatggtaatt gggtaactac gttccccgag
    13801 agtaacgaca atcctctcca taacgcgaca tccaccttaa ttactgtacc cgcagacgag
    13861 tgtatataaa gccagaatta gttgctttcc agcatgtgaa taatctggtt tgcaaccact
    13921 ttagcgcttt gatcatcagt acgaatggtc acgtcggcaa tctcttcata cagcggattg
    13981 cgttcattgg ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg cagcaacggg
    14041 cgttttttat cacgctgcgt gcgtgcaagt tgcttttcga tggtcgtttc aagataaacg
    14101 acaacgccac gagcggaaag acggttacgc gtttcacggg atttcacaga gccgccgcca
    14161 gtagccagca caataccctg tttctcggtc aactcattga tgaccttttc ttcgcgatcg
    14221 cggaagcctt cttcgccttc taaatcgaaa acccagccca catcagctcc ggttcgtttc
    14281 tcaatctctt gatcggaatc gtaaaattcc atattgagtt gttgagctaa ctggcgccca
    14341 atagtgcttt ttccggcacc cataggccca accagaaaga tattgcgttt ctctgccatt
    14401 ttttcggtac tactaagact attcgttaat gataaacccg cttcgctcag agagcgccgc
    14461 aggacatgaa ctgaaacctc ataagatatt gcgagagtca gactgaaaat tatctcaata
    14521 ctccagcggg tttggcaact gaataaatca cccatccctc tgcatatctg gtcgctgcaa
    14581 gcgcgctgcc ttgctaccac cgctctggcg attaatcacc gggtaagatt agcgtaaaaa
    14641 agacagcaaa atgccgcctg aatgataaat catcatcatg gaacgcaggc agcgaagagc
    14701 accaactctc aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca aatagatgaa
    14761 acacgttcag cgtaaaaaca ttaccgcttt tacggctgtt tactcactgg aaaccagtcg
    14821 tggcgtgata aacaccacta actcgcgtcg ttcatcttct tttccgtcat gacgaaataa
    14881 ttgcccgaac cagggaatgt cgccaagcaa cggtacgcta tcctgacccg atttattttt
    14941 acgggtaaaa atgccgccca gcgccaacgt ttctccgctt ttgacctcga cctgcgtttc
    15001 gatctcctgc ttatcaatcg ccaacacttc gccatcggcc tgctgtagca cctgccccgg
    15061 aacgttctgg ctgatgtgta atttcagccg gatgcgacct ttttgtaaca ccgtgggcgt
    15121 aacttccatc cccaggacgg cctctttaaa ttccaccgac gtcgcgccac tttccccgct
    15181 ggaaacctga tatggaattt cgctcccctg tttaatgctg gcaggctgaa gatgtgaggc
    15241 cagcagacgc ggactggcga taatatccag ctgctgtttt tgttcgagcg cggaaagctc
    15301 aagatccagt aaacgtccgt tgatgcgtcc aatgttaaaa ccgacatgcg ttgtcgccgt
    15361 cgctacggag aggtcgctgc caagcgtggt gacttgccca acgccaccag cttgctgcgc
    15421 atcagccaac gtccatttca cgcctaactc acgcaaactt ttttcattaa tggtgacaat
    15481 atgcgccgac agctcaacct gcccgaccgg cagatccatt tgcgctaccc actgttcaag
    15541 cgcgcttaac gccgttttgt tatctcgcag caaaaggcga ttggtgcgtt tatcgacggt
    15601 catactccct ttggcactca gtagcttctc cccggctttc gccagttctc ccgcgtcggc
    15661 gtattgcagg gttatactgc gattttccag cggcagattt gcctgcgccc gcgcctgctc
    15721 cgcctcctga cgggcgatat tgtcattctg ccaggcaacg gaatgcaccg agagaatgtt
    15781 gccctcctgg cgcgttatca atccggcgct tttcactaca gtttgtagtg cctgcttcca
    15841 gggaacgtca gttaaatgta acgacaccgt accgctgacg tctggcgaaa ccaccaggtt
    15901 caacttctcc tgttcagcca gcgcctgcaa cacctgagct accggaacgt catccaccat
    15961 cagcgtcact ttttgcggct ttgccgcctg tacgccgggt ataagcatca acagtagtgc
    16021 ggctatccat tgcttcattt gtatctcctt gccgttgcca caaccagtgt ggcggttcgc
    16081 agtttgtccc ggttcccagc gttagtgcgt ctggcgtcag ctgtaaaatt gtccagccgt
    16141 tttccagcac gtcgttttgc tgcacccgtc gccatttttt ttgcccgtct tttattacac
    16201 cgatgatgcg ctcacctcgc cctaccatcc cctgatagcg ccactggcta agttcgctaa
    16261 tccggcatag atcttccggc ggtttaaaag ggtcacgcat accggttaaa aggcacaatg
    16321 taatacctgc caacaaccag cgtttaaccc tcatgcggcg tctccagttg tagcgtgaac
    16381 aaaaggtcat caccttccac gcttaacgaa aaacggctca cgctgacgtt gcgctctgcc
    16441 agccgtgtaa atgccgacgg cactgcttcc cacagcgttt tcaacgccaa ctcacctccc
    16501 tgcgcggatg gatgccagta aaccagttgc gcgccggata actgaaaatc cagtggcgaa
    16561 aagggcagcg ttttttcctc gctgaaggga gtggtgtcaa ccaggcgata cagtgcggcc
    16621 cactgctggt gatgacttgc ccgtagtcga attaatgcgt cgctctcttc atggtgtgtc
    16681 gatgacagaa aaatgagcgt aactaacatc agcaaccaga ctgcccagca aagctggcgg
    16741 aggcggggtg atgtggcgaa ccaccagtca aagagcatgt ccatcgctaa ccttccttgt
    16801 taactgatac tcaaattgcc agcgtccctg cgcatcctgc tgcgtggccc cccgctgatt
    16861 gagatgaaaa gatgcatcct ggcggagtga cgtttccagc gcgtttaacg cggtaatgct
    16921 tgttgtcagc cccttgatct ccagcgtacc ttgctgccag cttatcgttg tcagccaggc
    16981 atgttcgggt aaaagcgccg ccagtgcttc cagcgcagat tgccagtcac gggtaaattg
    17041 tcgctggagc tgcctttgca aacgctgttc gcgtaactgc tggcgttcca gcaaacgtgg
    17101 tttcgttatc tgtaagctgc gggcgagttg ttgttccgcc tgaagcaaaa cggtgtctac
    17161 gcgtgcttcg gcgttgcttg tcagacgcag tattagcgtt atcccgacag ccagcagcag
    17221 aggcgcaacg aacatcagca accagaaacg cagaaaagcg gtccggcgtt gctgtcgcca
    17281 gggcaaaaaa ttaattggcg ggttcatcag tacgccttcc ctagcgccag ccccagcgcg
    17341 atggcaaagt ctccacctgg cggcggtagc ggcggctgac gaacagaaac cgcctcccag
    17401 ggatcaaatc cgccttcgcc acatatcgcg acgctttcgg gatcaacgga taacgctgcc
    17461 gccagctcct tcgcgctagt catccccacc gccagtttgc gcccccagcg atagcgcgtc
    17521 gcccacagcc actgttcgtt atcacgccag gccagacatt gctgatgaga aggtaaaaaa
    17581 ggcaggaatc gctgtaatgc actggcatcc ggggtgatcg cgctaacatg aacacgcaac
    17641 ctttctgcca gagtaagcag cgttgccaac tctttgcttt gcgcggcagt cacgttatag
    17701 gcgggactga gtgagtcttc gctgtaatcg aagcgcaggg agtccggatc catatccagc
    17761 tcgcgggcca tcgtcccgga cagccaggcc gtttgctccc gctcaccaag ggacatcgac
    17821 ggacgtggaa atgaccgctg taatgtgcga ctggcgggaa acgccaacat aatgtgatga
    17881 cgctgcggca gttcgcgact ccacggtaac aacgttttag ccagccgctg cacatcaaca
    17941 atccgcccat ctttgataat gtcgttctcc agcggcaacc gccaccagcg ttgcaaaaag
    18001 cattcttttg tgccccaacc gctaccgctt cttgctgttg taaatgcaaa ccaatttgcc
    18061 agatcttaaa tgccattgtg atgatctcct tatcacccgt cactctgacg ggtatatcaa
    18121 tgcgtctggc ttgcctttat actaccgcgc gtttgtttat aaactgccca aatgaaacta
    18181 aatgggaaat ttccagtgaa gttcgtaaag tattttttga tccttgcagt ctgttgcatt
    18241 ctgctgggag caggctcgat ttatggccta taccgctaca tcgagccaca actgccggat
    18301 gtggcgacat taaaagatgt tcgcctgcaa attccgatgc agatttacag cgccgatggc
    18361 gagctgattg ctcaatacgg tgagaaacgt cgtattccgg ttacgttgga tcaaatcccg
    18421 ccggagatgg tgaaagcctt tatcgcgacg gaagacagcc gcttctacga gcatcacggc
    18481 gttgacccgg tggggatctt ccgtgcggca agtgtggcgc tgttctccgg ccacgcgtca
    18541 caaggggcga gtaccattac ccagcagctg gcgagaaact tcttcctcag tccagaacgc
    18601 acgctgatgc gtaagattaa ggaagtcttc ctcgcgattc gcattgaaca gttgctgacg
    18661 aaagacgaga tcctcgagct ttatctgaac aagatttacc tcggttaccg cgcctatggt
    18721 gtcggtgctg cggcacaagt ctatttcggt aaaacggtcg accaactgac gctgaacgaa
    18781 atggcggtga tagccgggct gccgaaagcg ccttccacct ttaatccgct ctactcaatg
    18841 gatcgtgccg tcgcgcggcg taacgtcgta ctgtcgcgga tgctggatga agggtatatc
    18901 acccaacaac agttcgatca gacacgcact gaggcgatta acgctaacta tcacgcgccg
    18961 gagattgctt tctctgcgcc gtacctgagc gaaatggtgc gccaggagat gtataaccgt
    19021 tatggcgaaa gtgcctatga agacggttat cgcatttaca ccaccatcac ccgcaaagtg
    19081 cagcaggccg cacagcaggc ggtacgtaat aacgtgctgg actacgacat gcgccacggc
    19141 tatcgcggcc cggcaaatgt gctgtggaaa gtgggcgagt cggcgtggga taacaacaag
    19201 attaccgata cgctgaaggc gctgccgacc tatggtccgc tgctgcctgc cgcagtcacc
    19261 agcgccaatc ctcaggaagc gacggcgatg ctggcggacg ggtcgaccgt cgcattgagt
    19321 atggaaggcg ttcgctgggc gcgtccttac cgttcggata ctcagcaggg accgacgccg
    19381 cgtaaagtga ccgatgttct gcaaacgggt cagcaaatct gggttcgtca ggttggcgat
    19441 gcatggtggc tggcgcaagt gccggaagtg aactcggcgc tggtgtcgat caatccgcaa
    19501 aacggtgccg ttatggcgct ggtcggtggc tttgatttca atcagagcaa gtttaaccgc
    19561 gccacccagg cactgcgtca ggtaggttcc aacatcaaac cgttcctcta caccgcggcg
    19621 atggataaag gtctgacgct ggcaagtatg ttgaacgatg tgccaatttc ccgctgggat
    19681 gcaggtgccg gttctgactg gcagccgaag aactcaccac cgcagtatgc cggtccaatt
    19741 cgcttacgtc agggactggg ccagtcgaaa aacgtggtga tggtacgcgc aatgcgggcg
    19801 atgggcgtcg actacgctgc agaatatctg caacgcttcg gcttcccggc gcaaaacatt
    19861 gtccacaccg aatcgctggc gctgggttca gcgtcattca ccccaatgca ggtggcgcgc
    19921 ggctacgcgg tcatggcgaa cggcggcttc ctggtggacc cgtggtttat cagcaaaatt
    19981 gaaaacgatc agggcggcgt gattttcgaa gcgaaaccga aagtagcctg cccggaatgc
    20041 gatattccgg tgatttacgg tgatacgcag aaatcgaacg tgctggaaaa taacgatgtt
    20101 gaagatgtcg ctatctcccg cgagcagcag aatgtttctg taccaatgcc gcagctggag
    20161 caggcaaatc aggcgttagt ggcgaagacc ggcgcgcagg aatacgcacc gcacgtcatc
    20221 aacactccgc tggcattcct gattaagagt gccttgaaca ccaatatttt tggtgaaccc
    20281 ggctggcagg gtactggctg gcgagcaggt cgtgatttac aacggcatga tataggcgga
    20341 aaaacgggta ccactaacag ttcgaaagat gcgtggttct cgggttacgg tccgggcgtt
    20401 gtgacctcgg tctggattgg ctttgatgat caccgtcgta atctcggtca tacaacagct
    20461 tccggagcga ttaaagatca gatctcaggt tacgaaggcg gtgccaagag tgcccaacct
    20521 gcatgggacg cttatatgaa agccgttctt gaaggtgtgc cggagcagcc gctgacgccg
    20581 ccgccgggta ttgtgacggt gaatatcgat cgcagcaccg ggcagttagc taatggtggc
    20641 aacagccgcg aagagtattt catcgaaggt acgcagccga cacaacaggc ggtgcacgag
    20701 gtgggaacga ccattatcga taatggcgag gcacaggaat tgttctgatt aaaaaggcgc
    20761 ttcagcgcct ttttaagttt gctgacaaag tgcactttgt ttataccgga tacggcgtga
    20821 acactttacc cgtctaaaaa catctagaaa aatcaatata ttgcaggagc cacacaggcc
    20881 tgataagcat tatgcatcag gcaaacttca cgcatttaca accgcccctg ccccttcaac
    20941 cattcgcgca cgaggaaaag cgcgctgaca ttacgcgctt cgttgaagtc agggtcttcc
    21001 agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc tggctcatcg
    21061 ccttccagcg attccgggta gagatcttgc gctaccacga tattcatttt gctggaaaag
    21121 taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc aaatcccacc
    21181 tcttctttta gctcgcggtt cgcggcttcg aagacgcttt cacccggatc aattaatcct
    21241 ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat caggatcagg
    21301 tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg cattcgttca
    21361 taaacacgcc gcacgccatt gctgaactcc agatccacgc tttcgacggt aaacagtcgg
    21421 gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt gctcatcgtg
    21481 ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt cggcaatgtg
    21541 aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa tcaaattaaa
    21601 agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag agggtttgct
    21661 atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaaactcca cgcttgccga
    21721 tagccaaccg cagaatcatg ttttgtgtcc ggtgcgactg accacgcctg acagactaag
    21781 taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct ggcctgctca
    21841 ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc ctggaccaac
    21901 gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc cgttgaaaat
    21961 tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacgggagc cagcgcggca
    22021 ccgatttctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctaac acacgctatc
    22081 acgcgttacg gcatctctac cgacgatccg aataaatggc gttactacct cgattcggta
    22141 gaagtccacc tgcccccttt ctgggagcag tacatcaacg atgagaatac cgttgaactg
    22201 attcataccg attcgctgcc gctggttatt tcactcaacg gtcatacgct gcaggagtac
    22261 atgcaggaaa ctcgcggcta tgccttgcaa cctgttccgt caacacaggc gtcgattcgc
    22321 ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca cgaagagtat
    22381 gcgctaagtc gtccgcgcgg gctgcgcgaa gcgttgctga ttgtcgcctc cttcctgatg
    22441 ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg cggcgcgtta
    22501 ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc ctccctgcgg
    22561 gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga aaacgatcag
    22621 gaacagatca acaatatttc actcggtatt atcgacctgg tctaccccgc acactggcag
    22681 ccctacattg ctcatgatct cggccaacaa accgatatcg atatctatct cgaccgccac
    22741 gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt tccgttacag
    22801 cactggctgc gcagtacgat tatcgctgcg ggttcgctgc tggtgctgtt tatgctgtta
    22861 ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa aggcgcgcag
    22921 accattgaag ccaccagcgt gaaacaactg gctgacgctg gcgtacgggt tggcgataca
    22981 ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggtacctg gagcgcgaaa
    23041 accaactcac cttttttacc gtttgactgc tcgcagatca tctggaatga cgcccgctca
    23101 ttgccattac cagaatctga attggtcaac aaagcgacgg cattgaccga agcagttaat
    23161 cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gcgcctcatt acgttcagca
    23221 attcaaaaat ccggcatggt attgcttgat gattttggcg acatagtact gaagacagcg
    23281 gatctgtgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt caatctcggc
    23341 aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaaact cgatggcgtg
    23401 aatgtgttat tacgcccggt gagtgcggaa tcgctggata acctggtggc aacctccacc
    23461 gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag tccggccccc
    23521 ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc ctggccttcg
    23581 gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa actggcacaa
    23641 atgctgatgc atacgccgtt taacgccgag ggtatcgtca caaaaatctt cactgacgcc
    23701 aatggtacgc agcatatagg ccttcatccg atcccggatc gttccggcct gtggcgctat
    23761 ctcagcacca cgctgctgct actgacgatg ctgggtagcg ccatttataa tggcgtacag
    23821 gcctggcggc gttaccagcg tcatcgtact cgcatgatga agattcaggc ctattatgaa
    23881 agctgcctga acccacaact gatcacccct tcagaaagcc ttatcgaata acacgtttgc
    23941 gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt ccctcatgca
    24001 tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg gcacgttgct
    24061 cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat ggggcgcgaa
    24121 aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc acgacgtaca
    24181 gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc tggatatctg
    24241 tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca ttccgtttct
    24301 tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgcgc atccgcacaa
    24361 cctggcggta aaacttgagc ataccggtct ggacgcacac cttgatttat tactttccac
    24421 ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg ccgaagctac
    24481 gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc tcgatgctgc
    24541 cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgactccg ggattgccga
    24601 gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc cctcgctaat
    24661 gtgaaggaga cgccatgaaa gaaaaacctg ctgttgaggt tcgactggat aaatggctat
    24721 gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa ggcggtaagg
    24781 tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat gccacgctca
    24841 ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact gaacagcgtc
    24901 gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta gagaaacgcg
    24961 aaaaaatggc gctggcacgt aaacttaatg ccttaaccat gccgcacccg gaccgacgcc
    25021 cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt gaataactgt
    25081 cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat ctgtttgaaa
    25141 actttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag atccttgaga
    25201 accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt gcgaccagcc
    25261 tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcaa ggcgacggtc
    25321 cgatgaatct ggcggttatt aacggtaaca acaaccagca gatgcgcggt gtggcgcgcg
    25381 tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat ggttacgtgg
    25441 tgatcaccat caccccgagc gaaggtgaac gctatcaggg cgtggtgggt ctggaaggtg
    25501 atactctggc ggcctgcctg gaagattact ttatgcgttc tgaacagctg ccgacgcgcc
    25561 tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg ttgttgcagg
    25621 taatgcctgc gcaaaatgcc cagcaggacg actttgacca cctggcgacg ctaaccgaaa
    25681 ccatcaaaac cgaagaactg ttgaccttac cggcaaacga agtgttgtgg cgtttgtatc
    25741 acgaagaaga ggtgacggtt tatgatccgc aggatgtgga gttcaaatgc acctgctcgc
    25801 gtgaacgttg cgccgacgcg ctaaaaacgc tgccggatga agaagtcgat agcatcctgg
    25861 cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat ctgttcaatg
    25921 cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgcaa gttcattaat
    25981 gagtatgtcc ggcagagagg gctctgccgg attttacgac gtaatggcct gatgcgctta
    26041 tcagacctac ggattcaatg caatttatcg caggtaagat aatgtgccga atccaataaa
    26101 aatcagatat gcgctgtcat tcattcagat tcaaatccag tctttcgcca tccaccgtaa
    26161 gatcgcgcca cactttaggc ttcgtcccca gttttccatc ctccgcagcc atgagtaatt
    26221 tttctttcgt tattacagcc gggagtgaat tcatcgtaat ctctttccat tctttcccag
    26281 caggatcaat accatagata atgtatcttt catcattaaa ggcaaacagg atccgctctg
    26341 ccctgccatc actattcaga tcttgctcta ccagcacgca ggcatctttt tcaatgcata
    26401 tcattgcgtt atagcgattt ttcatcactg ccgaccagaa tatcgtatcc ggttttccag
    26461 aacctgaggc aataaataca ttatctgcta atgctttttc cgatatctgc tgctgaagac
    26521 gttgtactcc atcaaatgcc atcagcagac cccgcctgcg tttcgggtct ttcatatact
    26581 cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctc tgttcgagca
    26641 tatagagact tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc atatgactgt
    26701 tcacgctaat gcgcatactg tccagcaccg gagaattaag aagcaccagt attaccaaca
    26761 ccaataacga aaccgcaagg ttcactttgc cctgaagaac aagggggttt tgccctttac
    26821 gccagacgat actgacaaaa taccccagcg accataccag taacaccagc accgccagcg
    26881 cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat aacgcccagg
    26941 cggcgacgaa cacatacagc ggagcaacca gcaaagcggt tttaatcagg caacgcaacg
    27001 gccccgtcca gggaagagac gctttttgcg gatcgcggac aatagccatt aagatcaatt
    27061 gcaaaaaggc cagcgttaac agcaacccag cggcggagat gtggcgagaa atcgcgctca
    27121 ggcccgtaaa cggcagggtg ataataaaca tcagggttag caatgatact aacggcagca
    27181 accccgtggc gattaacgtg aacaactttt gaatagagtc gattaaacgt gactgtgtac
    27241 gcgcgaggat caccgccagt gcggtaacca gacctaacgt gagataaata aaccagtcgg
    27301 ttgcaaaaaa gagcgttttg aaaaacgtga tgccaacaag tttaaacaac tctccccaca
    27361 gtaaaagtac cagccacgtc aggccattgg cgagaaaaat caccagtaat ataagtacgt
    27421 tatgccatac tgactggtaa aaatagcgat agcggctact gtcattgcgg atgcgaaggc
    27481 tttgttgtat ccacggtagc aacaacattg ccatcagcag cagatagcaa ccaaaatccc
    27541 aaagcgcctt ttctgctctc caggggttca tgccatcaat ttgccacttt agccaaccgc
    27601 tcatccctag cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa
    27661 aggagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa
    27721 caatccaact gtgatttttt ccggcaagcc aggcgatcag caggtagcaa accagaccct
    27781 gaagtaatcc ggtagcaatc atcccccagc gtgttgccgg tgaaagttca acgttatcca
    27841 tgttagttat ccagaatcaa aaagtgggtt aattattgca tccgggccgt agtattttgc
    27901 ttttttcaga aaataatcaa aaaaagttag cgtggtgaat cgatacttta ccgattgaat
    27961 ttgcatcaat ttcattcagg aatgcgattc cactcacaat attcccgcca tataaaccaa
    28021 gatttaacct tttaagaaca ttttccacac ctaaaatgct atttctgcga taatagcaac
    28081 cgtttcgtga caggaatcac ggagtttttt gtcaaatatg aatttctcca gatacgtaaa
    28141 tctatgagcc ttgtcgcggt taacaccccc aaaaagactt tactattcag gcaatacata
    28201 ttggctaagg agcagtgaaa tgcgcgttaa caatggtttg accccgcaag aactcgaggc
    28261 ttatggtatc agtgacgtac atgatatcgt ttacaaccca agctacgacc tgctgtatca
    28321 ggaagagctc gatccgagcc tgacaggtta tgagcgcggg gtgttaacta atctgggtgc
    28381 cgttgccgtc gataccggga tcttcaccgg tcgttcacca aaagataagt atatcgtccg
    28441 tgacgatacc actcgcgata ctttctggtg ggcagacaaa ggcaaaggta agaacgacaa
    28501 caaacctctc tctccggaaa cctggcagca tctgaaaggc ctggtgacca aacagctttc
    28561 cggcaaacgt ctgttcgttg tcgacgcctt ctgtggtgcg aacccggata ctcgtctttc
    28621 cgtccgtttc atcaccgaag tggcctggca ggcgcatttt gtcaaaaaca tgtttattcg
    28681 cccgagcgat gaagagctgg caggtttcaa accagacttt atcgttatga acggcgcgaa
    28741 gtgcactaac ccgcagtgga aagaacaggg tctgaactcc gaaaacttcg tggcgtttaa
    28801 cctgaccgag cgcatgcagc tgattggcgg cacctggtac ggcggcgaaa tgaagaaagg
    28861 gatgttctca atgatgaact acctgctgcc gctgaaaggt atcgcttcta tgcactgctc
    28921 cgccaacgtt ggcgagaaag gcgatgttgc ggtgttcttc ggcctttccg gcaccggtaa
    28981 aaccaccctt tccaccgacc cgaaacgtcg cctgattggc gatgacgagc acggctggga
    29041 cgatgacggt gtgtttaact ttgaaggcgg ctgctacgca aaaactatca agctgtcgaa
    29101 agaagcggaa cctgaaatct acaacgctat ccgtcgtgat gcgctgctgg aaaacgtcac
    29161 cgtgcgtgaa gatggcacta tcgactttga tgatggttca aaaaccgaga acacccgcgt
    29221 ttcttatccg atctatcaca tcgaaaacat cgttaagccg gtgtccaaag cgggccacgc
    29281 aactaaagtt atcttcctga ccgcagacgc ctttggtgtg ctgccgccgg tttctcgtct
    29341 gactgccgat caaacccagt accacttcct ctctggcttc accgccaaac tggccggtac
    29401 agagcgcggc atcaccgaac cgacgccaac cttctccgcc tgcttcggcg cggcattcct
    29461 gtcgctgcac ccgacgcagt acgcagaagt gctggtgaaa cgtatgcagg cggcaggcgc
    29521 gcaggcgtat ctggttaaca ccggctggaa cggcactggc aaacgtatct ccattaaaga
    29581 tacccgcgcc attatcgacg ccatcctcaa cggttcgctg gataacgcag aaaccttcac
    29641 tctgccgatg tttaacctgg cgatcccaac cgaactgccg ggcgtagaca ccaggatcct
    29701 cgatccgcgt aacacctacg cctctccgga acagtggcag gaaaaagctg aaacgctggc
    29761 gaaactgttt atcgacaact tcgataaata caccgacacc cctgcgggtg ccgcgctggt
    29821 agcggcaggt cctaagctgt aatcagctga tatctcattg accccttatc caaacggata
    29881 aggggttttt ttatctaatt agctaccaat atgtacacgg tacacttata attatcaata
    29941 gaccgcagga tgcattatgt tcacctttat tgaactacaa ggatttagca aacgccgcca
    30001 gttattgcta cctgatgatg agttccgggc ttttcaggaa ctactcattc aagatcccac
    30061 tgccggtgac attatcgcag gcacaggcag ttttagaaaa attcgctgga accgttctgg
    30121 aatggggaaa cggggtggca ttcggataat ttattacaac gtaacacgca atggccgaat
    30181 ttatctggcg ttgatttatc ctaaaaatga acaggatgat ttaacagaag aacaaagaaa
    30241 ggcattgaaa ctattgtccg agaagctgct ttaatgttga agctccacaa attccaccat
    30301 ggccgaaatg tcaacggagg cattgaaaac agatgaaaga tgaattattt gctgatcttc
    30361 ttgccagcgc agaagaaatg gtacgcatcg aaaagggtga agaaacgcct gaaccagagc
    30421 acgtccatac ttttagcgaa atcgatgtaa aagcgatccg tgaagcaacg ggtttacgtc
    30481 agcaagattt tgctgtcgcc gtaggggtaa gttatgacct ggtcaaaagc tgggaaacca
    30541 aacgccgtca acctaccgga gcaccccgaa aattgctcct gcttttgcaa gcgaatcctt
    30601 tcattatcaa tcaattgaaa acaatctaaa aacaggaggc accttcgcct cccgttttat
    30661 ttacccttct tttgtcgtgc cctgcgcccg cgttaccggc actggcagcc aggcgcgaat
    30721 ggaaagcccg ccccgctcgc tgctgccaag ctccagcatg ccgttatggt tatccacgat
    30781 acgctgcaca atcgccagcc ctaatcccgt gccgctaatg gtgcgcgcac tgtcgccgcg
    30841 aacaaacggc tggaacaggt gcttacgttg ttccggcgca attcccggac catcatcttc
    30901 cacctggaac caggcgcgat tcggctccgt tccgctgctg actttgatcc agccattgcc
    30961 atagcgggca gcgttcacca ccatattcgc caccgcacgt ttgatcgaca gcgggtgcat
    31021 tttcacctca atgctgccgg ggtaaagcgc ggtttcaatt tcccgctcat agccactttc
    31081 ggcggcaatc acctcaccga gcactgcgtt gagatccgcc atttccatcg gcatctcctg
    31141 cccggtgcgc aggtagtcga taaactgctc aatgatggcg ttgcactctt cgatatcttt
    31201 attgatcgat tcggccaggt agccatcctg ctcgctcatc atctcggtcg ccaggcgaat
    31261 gcgcgtcagc ggcgtacgca gatcatgact tacccccgcc atcagcagcg tgcgatcatc
    31321 cgccagttgc ttaacaccag ccgccatatg gttaaaggcg cgggtaacgg aacgcacctc
    31381 cgaagcgcca tattcacgca gcggcggcgg aataatccct ttaccgacct gcagagcagc
    31441 atgttcgaga tcgaccaacg gtcggttctg gatacggata aacagccacg ccccgcctat
    31501 cgccaatagc ataatcgcca gcgtatagcg gaacagcgga gaaaaatcac cctgatgaat
    31561 ttcggtcagc ggcacgcgca cccagatatt gggcgacagc caggttttca gccagacaac
    31621 aggcgaactt ttgttgacct caacgcgcac ttccgtcggg ccgcccagtt gctgcgccat
    31681 ctgatggctt aagaattcat agtgttgtgc ccaacgcaga cctgcctctt cggcagcttc
    31741 gttggaatag agagagatcc ccagctcacg gtagatctcc cgacggaaag cgggaggcac
    31801 aaccaactgc gtgccgtcct ccagttgcag tttgtcggtc atcaacatac gcacttcgta
    31861 cgcgaggact ttattaaact gctggaggct cggcaaaatc gcgaagttca gcaccaccag
    31921 ataagtcgtc accaggctga cgaacagcaa ggtgacgatg agcaataacg tacgggcaaa
    31981 tgaacttcgt ggcgagaagc gcaatcgcct catgctttag aaccgtccgg tacaaagacg
    32041 tagcccagac cccagacggt ctgaatgtaa cgcggatgcg ccggatcttc ttccaccatg
    32101 cggcgtagac gcgaaatctg cacgtcgatg gagcgttcca ttgcggagta ttcacggcca
    32161 cgggcaaggt tcatcagctt atcgcgggag agcggctcac gcggatggct gaccagtgcc
    32221 ttcagtaccg caaactcacc gctggtgagc ggcatcggct catcttcgcg gaacatttca
    32281 cgcgtaccga ggttaagttt gaacttaccg aaagcaatta ccgcctcttc ctgtgacggc
    32341 gcgcccggca gttcgttggc ctgacgacgc agcaccgcac gaatgcgggc cagcaattca
    32401 cgcgggttaa acggttttgg aatgtagtcg tcagcgccaa tctccaggcc aacgatacgg
    32461 tccacttctt cccctttcgc cgtcaccata atgatcggca tcgggttgct ctggctacga
    32521 agacgtcggc aaatcgacaa gccatcttca ccaggtaaca tcaaatccag taccataaga
    32581 tggaaagatt cacgagtcag caggcgatcc atctgttctg cattagcgac gcttcgaacc
    32641 tggaagcctt gttcggtgag ataacgttcc agcagcgcgc gcaggcgcat atcgtcatcg
    32701 accaccagaa tcttgtagtt ctcttgcatt gtttgtactc ccaaaggttc gcaacaattt
    32761 gtaagcgtgt attcttaaaa aagctcacgt tcgtcaccag ctaaatctgg tatgaatttc
    32821 agcctaaatt gttacaaagc atattaaaca gcagcttaag tatacaattt attcggcgaa
    32881 acattattga ttctgttgat atgatcacgt tatacccaat gtgcgcatta tcaaacagac
    32941 aaagggaatc aacgagatga aaacgcccct ggttacccgg gaagggtatg aaaaactcaa
    33001 acaagagctt aattatctct ggcgtgaaga acgcccggag gtcacaaaaa aggtgacctg
    33061 ggccgcaagt ctgggcgacc gcagcgaaaa tgctgactat cagtataata aaaagcgcct
    33121 gcgtgaaatc gaccgtcgcg tgcgctatct cactaaatgc atggaaaatc tcaaaatcgt
    33181 cgattactcc cctcagcagg aaggcaaagt cttttttggt gcgtgggtgg agattgaaaa
    33241 cgacgatggc gtgactcacc gtttccgtat tgtcggctac gatgaaattt ttggccgtaa
    33301 agattacatc tctatcgatt ccccgatggc tcgcgcattg ttgaaaaaag aggtgggcga
    33361 tttggcggtg gtgaataccc ctgccgggga agcgagctgg tatgtgaatg ctatcgagta
    33421 cgtgaaaccg taaggaagag tcttaacctc ctgccgatgg ctggcatttt tgccagccag
    33481 tccgtataac tatcccctga tttttgatcc gaaaagatga actcaaacca tgatgaatga
    33541 ttcgttctgc cgcattattg cgggtgaaat tcaggcgcgc ccggaacagg ttgacgctgc
    33601 cgttcgcctg cttgacgaag ggaataccgt gccgtttatc gcacgttatc gtaaggaaat
    33661 caccggcggt ctggatgaca cgcagctgcg taatctggaa acgcgtctga gctatctgcg
    33721 cgagctggaa gagagacgtc aggcgatcct caagtccatt tccgagcaag gcaaactcac
    33781 tgacgagctg gcgaatgcca tcaacgccac actaagcaaa accgaactcg aagacctcta
    33841 cctgccctac aaacctaaac gccgcacccg cgggcaaatc gccattgaag cagggcttga
    33901 gccgttggct gacctgctgt ggagcgatcc gtcacacacg ccagaagtcg ccgctgcaca
    33961 atatattgat gccgataaag gcgtggcaga taccaaagcc gcgctggacg gcgcgcgcta
    34021 tatcctaatg gaacggtttg ccgaagatgc cgcgctgctg gcgaaagtgc gtgattatct
    34081 gtggaagaac gcgcatttgg tctcaaccgt ggtgaatggt aaagaagagg aaggggcgaa
    34141 attccgcgac tatttcgatc atcacgaacc gctgtccacg gtgccttctc accgcgcgct
    34201 ggcgatgttc cgtggtcgta acgaaggcgt acttcagctt tcgctgaatg ccgatccgca
    34261 gttcgaagag ccgccgaaag agagctattg cgagcaaatc atcatggatc accttggcct
    34321 gcgcctgaac aatgccccgg cggatagctg gcgcaaaggt gtggtgagct ggacgtggcg
    34381 catcaaggtg ttgatgcatc tggaaactga actgatgggc accgtgcgcg aacgcgcgga
    34441 agatgaagca atcaacgtct ttgcccgtaa cctgcacgat ctgctgatgg cggcccctgc
    34501 cggactgcgt gcaacgatgg gcctcgatcc gggtctgcgt accggggtaa aagtggcggt
    34561 agtcgatgcg acaggtaaac tggtggcgac cgataccatt tatccgcata ccgggcaggc
    34621 cgcaaaagca gcgatgaccg ttgctgcgct gtgtgaaaag cataacgttg aactggtggc
    34681 gatcggcaac ggtacagctt ctcgcgaaac cgaacgtttc tatctcgacg tgcagaagca
    34741 gttcccgaaa gtgaccgcgc agaaagtgat cgtcagcgaa gctggcgcgt cggtttactc
    34801 ggcttccgag ctggcagcac aggaattccc ggatctcgac gtttcgctgc gtggcgcggt
    34861 gtctatcgcc cgtcgtttgc aggatccgct ggcggagctg gtgaaaatcg atccgaaatc
    34921 tatcggcgta ggtcagtatc agcatgacgt cagccagacg cagctggcac gcaaactgga
    34981 tgcagtagta gaagactgcg taaacgccgt tggcgtcgat ctcaacaccg cttctgttcc
    35041 tctgttaacc cgcgtggcgg gcctgacgcg catgatggcg caaaacatcg tggcctggcg
    35101 cgatgagaac ggccagttcc agaaccgtca gcaactgtta aaagtgagcc gtctggggcc
    35161 gaaagccttc gagcagtgcg cgggcttctt gcgcattaac catggtgata acccgctgga
    35221 cgcgtctacc gtccacccgg aagcctatcc ggtggtggaa cgcattctgg cagcaacaca
    35281 gcaggcactg aaagatctga tgggtaacag cagcgagctg cgtaacctga aagcgtctga
    35341 ctttactgat gaaaaattcg gtgtgccgac ggtaaccgac atcatcaaag agctggaaaa
    35401 accgggtcgc gatccgcgtc cggaatttaa aaccgctcag tttgccgatg gcgtcgagac
    35461 aatgaacgac ctgcaaccgg gtatgatcct cgaaggtgcg gtgaccaacg tcaccaactt
    35521 tggcgcgttt gtcgatattg gcgtgcatca ggacggcctg gttcacatct cttcattgtc
    35581 gaacaagttt gtggaagatc cgcataccgt ggtgaaagcg ggcgacattg tgaaggtgaa
    35641 agtgctggaa gtggatcttc agcgtaaacg tatcgccctg accatgcgtc tggacgagca
    35701 gcctggcgaa accaacgccc gtcgcggtgg cggtaacgat cgcccgcaaa acaaccgccc
    35761 ggcagccaaa ccgcgtgggc gtgaagcgca gcctgccggt aatagcgcga tgatggatgc
    35821 gctggcggcg gcaatgggca aaaaacgtta aacgcccgga ctggccccta aacctgcgcg
    35881 attaggcgtt cacgccacat ctggcaacga acgccttgcc tgacataaaa gtgccggaga
    35941 atatctccgg cctttttatt ccacaagcaa actcataata tatcccatta atatttatca
    36001 cttcattaac aactgaaacc ttaattaaac attaaccagc cctaataatt cgtcatgcga
    36061 attatatttt cgctgcgata taaccttgag ccatatcaac attgagtcag attattattc
    36121 aaaccaacat tcacacacat tttaagtatt gctgatagaa accattctca ttatcattgt
    36181 actgttgatt atttaatctc tccttcgttg gcaaatcatc tggtctcatg tcgctgtcaa
    36241 acgccccatg aggtagttat ccagttaatg agaaacaagt aggcacctat gcaatacact
    36301 ccagatactg cgtggaaaat tactggcttt tcccgtgaaa tcagcccggc atatcgccaa
    36361 aaactgcttt ctcttggcat gttacctggc tcctctttta atgtggtgcg cgtcgctcca
    36421 ctcggcgacc ccattcatat cgaaacccgt cgtgtgagcc tggtattacg aaaaaaagat
    36481 ctggccttat tagaagtgga agcggtttcc tgttaatacg gtgataacaa caatgaaaaa
    36541 attaaccatt ggcttaattg gtaatccaaa ttctggcaag acaacgttat ttaaccagct
    36601 cactggctca cgtcagcgtg taggtaactg ggctggcgtt accgtcgaac gtaaagaagg
    36661 gcaattctcc accaccgatc atcaggtcac gctggtggac ctgcccggca cctattctct
    36721 gaccaccatc tcatcgcaga cctcgctcga tgagcaaatc gcctgtcact acattttgag
    36781 tggcgacgca gacctgctga ttaacgtggt ggatgcgtct aaccttgagc gtaacctgta
    36841 cctgacgctg caactgttgg aactcggcat tccctgcatc gtggcactga acatgctcga
    36901 cattgccgag aagcaaaata ttcgtattga aattgatgct ctgtcggcgc gtcttggctg
    36961 cccggtgatc ccgctggttt caacccgtgg tcgcggtatt gaagcgctca agctggcgat
    37021 tgatcgctat aaagctaacg agaatgtgga actggtgcat tacgcacagc cgctactcaa
    37081 cgaagcagat tcactggcaa aagtgatgcc ttccgacatc ccgctgaaac aacgtcgctg
    37141 gctgggcctg caaatgctgg aaggcgatat ctacagccgc gcctacgccg gtgaagcgtc
    37201 gcagcatctg gatgccgccc tcgcccgtct gcgtaatgag atggacgatc cggcgctgca
    37261 cattgccgat gcgcgttacc agtgcattgc tgccatctgt gatgtggtaa gcaacaccct
    37321 gacggcagaa cccagccgtt tcaccactgc ggtagataaa atcgtgctca accgtttcct
    37381 cggtctgccg attttcctct ttgtgatgta cctgatgttc ctgctggcta tcaacatcgg
    37441 cggggcgtta cagccgctgt ttgatgtcgg ttccgtggcg ctgtttgtgc atggtattca
    37501 gtggattggc tacacgctcc acttcccgga ctggctgact atcttcctcg cccagggcct
    37561 gggtggcggt attaacaccg tgctgccact ggtgccgcag atcggcatga tgtacctgtt
    37621 cctctccttc cttgaggact ccggctatat ggcgcgtgcg gcgtttgtga tggaccgcct
    37681 gatgcaggcg ctgggcttgc cggggaaatc ctttgtaccg ctgatcgtcg gcttcggttg
    37741 taacgtacca tcggtaatgg gcgcacgtac gcttgatgca ccgcgtgaac gcctgatgac
    37801 catcatgatg gcaccgttta tgtcctgcgg cgcacgtctg gctatcttcg cagtattcgc
    37861 ggctgccttc ttcgggcaga acggtgcgct ggcggtcttc tcgctgtata tgctgggtat
    37921 cgtaatggcg gtgctgacgg gcctgatgct caagtacacc atcatgcgcg gtgaagcgac
    37981 gccgtttgtc atggagctgc cggtctacca tgtaccacac gttaaaagcc tgattatcca
    38041 gacctggcag cgtctgaaag gcttcgttct gcgtgctggt aaagtgatta tcatcgtcag
    38101 cattttcctg agcgccttca acagcttctc gctgagcgga aaaatcgtcg ataacatcaa
    38161 cgactcggcg ctggcttccg tcagccgggt gatcacccca gtcttcaagc cgattggcgt
    38221 gcatgaagat aactggcagg caacggttgg cctgtttaca ggcgcaatgg cgaaagaagt
    38281 ggtggtgggc acgctcaaca ccctctacac cgcagaaaat attcaggacg aagagttcaa
    38341 tccggcagaa tttaacctcg gtgaagagct gttcagcgcg gtagatgaaa cctggcagag
    38401 cctgaaagac accttcagcc ttagcgtact gatgaatccc attgaagcga gcaaaggcga
    38461 cggcgaaatg gggaccgggg cgatgggcgt gatggatcag aaattcggta gcgcagctgc
    38521 cgcatacagc tacctgattt tcgtcctgct gtatgtaccg tgcatctcgg tgatgggggc
    38581 tatcgcccgt gaatcaagcc gtggctggat gggcttctcc atcctgtggg ggctgaatat
    38641 cgcttactca ctggcaacat tgttctatca ggtcgccagc tacagccagc acccaactta
    38701 cagcctggtg tgcattctgg cggttatcct gtttaacatc gtggttatcg gtttgctgcg
    38761 tcgcgcgcgt agccgggtgg atatcgaact gctggcaacg cgcaagtcgg taagcagttg
    38821 ctgcgcagcc agcaccaccg gtgattgcca ttaatggctt cacttattca ggtgcgtgat
    38881 ttgctggcgt tacggggccg tatggaagcg gcccagataa gccagacatt gaacaccccg
    38941 cagccaatga ttaacgccat gctgcaacag ctggaaagca tgggcaaagc cgtgcggatt
    39001 caggaagaac ctgacggctg cctctctggc aattgtaaaa gctgcccgga aggcaaagcc
    39061 tgcctgcgcg agtggtgggc gctgcgttaa ccatactcca tcacctgttt tgaaaagccc
    39121 ggtatgcgtt tgcatccggg cttttttgcg tgcggctttc cataaaaatg caacccttgc
    39181 agcgcgacgt aaattcctct gaaagcgtct cgcagggatg aaaactcgct aatgcacagg
    39241 tgtggagtgg tgcgtagagt cgcggcattc aaacaacagg tgaaggaacg ccatgagcaa
    39301 aaagcagagt tccaccccac acgatgcgct gttcaaactc tttttacgcc aaccggagac
    39361 ggcgcgcgat tttcttgcgt ttcatttacc ggcacctatt cacgcgctct gtgatatgaa
    39421 aaccctcaag ctggagtcga gcagctttat tgatgacgat ctgcgtgaaa gctattccga
    39481 cgtgctgtgg tcggtgaaaa cggaacaagg gccaggatat atctattgtc tgattgaaca
    39541 tcaaagcacc tcaaacaaac tgatcgcatt tcgcatgatg cgttacgcca ttgccgcaat
    39601 gcaaaatcac ctggatgccg gatacaaaac attgccgatg gtggtgccat tgttgtttta
    39661 ccacggtatt gaaagcccct atccctattc gctgtgttgg cttgattgtt tcgccgatcc
    39721 caaactggca aggcagcttt atgcctccgc atttccgctg attgatgtca ccgtcatgcc
    39781 tgatgatgaa atcatgcagc accgacgtat ggcgctgctg gagttaattc aaaaacatat
    39841 tcgtcaacgc gatctgatgg ggctggtaga gcaaatggcc tgcttattaa gtagtggata
    39901 cgctaatgac agacaaatca aagggctgtt taattacatc ttgcaaaccg gcgatgcggt
    39961 acgttttaac gattttatcg acggcgttgc cgaacattca ccgaaacaca aggagagctt
    40021 aatgactatt gcggaaagat tgcggcaaga ggggtttcag aaaggtatac gagctatcgc
    40081 caaaaccatg ctggatgccg gagtccctct tgaggacgtt ctgcgattta ccagcctgac
    40141 ggttgaagac cttgctgaaa taaatcatca ataacgtaaa ggatggtttt aatgacagat
    40201 gcagaaagag aatatcttat tagttacctg attggttatt tattgggtcg acgcctggaa
    40261 gccctggata tcgccaaaag aatgctgtaa tccggagtcc ctcttgcaga catcatgcgc
    40321 tttaccgggg tgtcagaaga agagttggct gcggcgagcc agtaaagttc tgtctcgcca
    40381 tttcaaaagc cacctacacc ctctgcttca acgccaccag catgcgacaa aactcggccg
    40441 gatgcgaaat aaacggcgca tgggccgctt tggcgaagat gtatgattcg ctgtgaggcc
    40501 aaagtttatc cagcatcggc accactttgc gcggcaccag accgtcgaga tagccataca
    40561 atcgcaaaaa cggcatggac acgttttgca gcggctgacg gagatcgacc attttcagga
    40621 tttccagccc gccattaagc acgtcaacct ccggcatcgg taacgccaga acggttttct
    40681 tcagcgcccg cgcatcctgg cgcgccgttt cagtacccat ggtttgtaac gccaggaacc
    40741 gctccaccgt acgctgaaaa tcatcactga gttgctgctg aaatcccgcc agcacgtccg
    40801 gttttatccc cggccactcg tcacgagcac taaaacaagg tgacgacgcc acggtgacca
    40861 gcgcctgaac acgctcggga tgggttaacg caatctggct tgccaccagc ccgcccagac
    40921 tccagcctaa ccaaatggct ttatcaggtg cctgtcgcag cacggcttcg gccatatcag
    40981 caagtgacat cgcaccaaat ccccggctac gcccgaagcc gggcagatca acaaggtgca
    41041 gcgtaaaatg cgagctcagt tcctcgtcaa tgcaacgcca cacttcggca ttcagtcccc
    41101 atccgtgcag cagcacaaga tgaacattcc cctgaccttt ggtctgccac cagatgttat
    41161 tcatccgcta ttgttctctt ttgacttaca aggatgaaca tatgctaaca gtaccgggat
    41221 tatgctggct atgccgaatg ccgctggcgt taggtcattg ggggatttgt tcggtatgct
    41281 cacgcgccgc cctggcaaag actggtcacg gttgccgact atgcgccgcc gttaagtccg
    41341 cttatccacc agcttaagtt ttcccggcgc agcgaaatcg ccagcgccct ggcacgtctg
    41401 ttactattgg aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc ggatcgcatt
    41461 atcagcgttc cgttatggca gcagcgtcac tggcgtcggg gatttaatca gagcgatttg
    41521 ctgtgtcagc cgttatcacg ctggttgcac tgccgatggg atagcgaagc cgtcacacga
    41581 acacgggcca ctgcgaccca gcattttctc agtgcccggc tgcgcaagcg caacctgaaa
    41641 aatgcctttc gccttgaatt gcctgtgcaa ggtcgccata tggtgattgt ggatgatgtc
    41701 gtcaccaccg gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg
    41761 actgtccagg tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatgg gcgtattata
    41821 accaactaaa atagtcaact attaggccat tactatgatc cgtatttccg atgctgcaca
    41881 agcgcacttt gccaaactgc tggcaaatca ggaagaaggg acacaaatcc gcgtatttgt
    41941 gattaaccct ggcacgccta acgctgaatg tggcgtttct tattgtccgc cggacgctgt
    42001 agaagccacc gacactgccc tgaaatttga cctgctgacc gcgtatgttg atgagttaag
    42061 cgctccttac ctggaagatg cagagatcga ctttgttaca gaccagttgg gttcccagtt
    42121 aacgctgaaa gcgcctaacg ccaaaatgcg taaagtcgca gacgatgcac cgctgatgga
    42181 gcgcgtggag tatatgctgc agtcgcagat caacccacag cttgctggtc acggtggtcg
    42241 cgtttcgctg atggaaatca ccgaagacgg ttacgccatt ctgcaatttg gcggcggctg
    42301 taacggttgt tctatggtag atgtgacgct gaaagaaggg atcgagaagc agctgctgaa
    42361 cgaattcccg gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg gcgagcactc
    42421 ctactactaa gttatcccct cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc
    42481 gggcctacgt tgaacatgtg ccagacgttg gcgcagcatt gctttggcaa acccctccct
    42541 tcgtttcccc gcgataatat gaccaacctc tcataattta aatttacccc gctctggtga
    42601 ttctcaaacg ccggatgtta cccgtatcat tcacgtgggt accaaacata ctcctgacat
    42661 ctgactacaa taattagttt tagtgggtat cagtcgtggt gccgcaatat ctctgttccc
    42721 gattgggata attagagttt gtcgtcagaa aattgacgtt acccataaca aatgaaaggc
    42781 caggtaaatc atgccattag tcattgttgc tatcggtgta atcttgttgt tgctcctgat
    42841 gatccgtttc aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg ctgttggatt
    42901 aatgcaagga atgccgctgg ataaagttat tggctccatc aaagcaggtg tcggcggaac
    42961 gctcggtagc cttgccctga tcatgggttt tggcgcaatg ctgggcaaaa tgctggcaga
    43021 ctgcggtggc gcacaacgta tcgccaccac gctgattgct aaatttggta aaaaacacat
    43081 ccagtgggcg gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtggg
    43141 ctttgtgctg atgctgccgc tggtgtttac catcgcggct tccgcgaata ttccactgct
    43201 gtatgttggt gtaccaatgg cggctgcact gtctgtgacc cacggcttcc tgccaccgca
    43261 tccgggcccg actgcgattg ccaccatttt caatgccgat atgggtaaaa ccctgctgta
    43321 cggtactatt ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgct
    43381 gaaaggtata gataagccga tcccggaagg tctgtacagc gcgaaaacct tcagcgaaga
    43441 agagatgcca agttttggcg tcagcgtctg gacctctctg gtcccggttg tgctgatggc
    43501 gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga
    43561 gttcctcggt gacccggtaa tggctacgct gattgccgta ctgattgcga tgttcacctt
    43621 tggtctgaac cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa
    43681 aatcattgcg atgatgctgt tgattatcgg tggtggcggt gcgttcaagc aggtgctggt
    43741 agacagcggc gtggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct
    43801 gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc
    43861 ggcaatcact gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccgga
    43921 gctgatggtt attgcggttg gttccggtag tgtgattttc tctcacgtga acgatccggg
    43981 cttctggctg ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc
    44041 gatgctggaa accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt
    44101 gatttgataa cactgactgc cggatgcatc gcatccggca tcaaccgcac tctacttctt
    44161 cttcgctgcc gctctgcgcc gtctgtccaa atccttcagc aacttgttca caccatcatc
    44221 ggcaaacatc gcctcaagcg ttgcggagag cttgcgccgc cagtttttat actggtaact
    44281 ggtgccggga atatttaccg ggtccgccat atccagccag tcttctggct gtagtcctaa
    44341 cagggcgctg ttgctatcgg cgatgtagcg ctgtaagcca cgattcagcg tcggtgtcat
    44401 cgacatcaac gatgccttat gcccggcacg ttttggcagg caaccatatt tatgcagagc
    44461 atccagtagt ccttgcttcg ccagttcgcg atcctgatac agaccgcgca gtaccacttc
    44521 atccggatac agccccagcg ttttgcccag cgttaaatcc ccgctctccc agtagccgcg
    44581 tagcgtaggc aggtcgtgtg tcgccgcaac cgccatcgac tgctctgggt acgctttcgg
    44641 tgcgcggaat gtcttctcgt ggtcgttttc gaaatagagc actttgtagg agtacacacc
    44701 gctgctgcgc agcttaccga caatctctac cggtacggta ccgagatctt caccaatcac
    44761 catacagcga tgacgtttac tttcgagtgc cagaatcgag agcagatcat ccaccggata
    44821 gtgaacatac gcgccctgat ctgccgtctc gccatacggt atccaccaca aacgcagcat
    44881 cgacatcacg tgatcaattc gtaatgcgcc gcagttttgc atattggcac gcaacagctc
    44941 gataaacggt tcataggcac gcgcggtgat aatatgcggg tccattggcg gcaatcccca
    45001 gttctgcccc aacgggccga ggatatccgg cggtgctcca accgacgctt tcaggcaata
    45061 caactcccgg tcgcaccagg tttctgatcc accctctgct acaccaaccg ccagatcacg
    45121 atacaagcca atcggcattt catagccctg gcttatctcc cagcaggcgg caaactggct
    45181 gtaagccagc cactgcaacc agagataaaa atcgacgtca tcacgatgtt cttcgcagaa
    45241 ctggcgcact tctggtgaat ccacgttctg atacatctct ggccatgcag gccagcccca
    45301 gcgcatttcg tcctctttca cttgctgggc atgtagcgcg tcaaacgccg cctgccagaa
    45361 caggctatcg ccttgctcgg caacaaactg acgaaacgcg gccacctgtt catcatcacg
    45421 ttgcgcgaaa cctttccacg ccatgcgcaa tgctgtcatt tttagggcgg taaccgtgga
    45481 gtaatcgacc cagtcggcat cccgcgtctg ttgcagcgtc tgttgcgtgg tcggcaactt
    45541 ccaccaggcc tgagcctctt cgctaagatg gaaatcttcg acggcgttaa cgtcgatata
    45601 aatcacattc agccaacggc gagaagacgg gctgtatggg ctggcacttt ccggatttgc
    45661 cggatagagc gcatgaatcg ggttcagacc gatgaacgac ccgccacgtt ttgccacatc
    45721 caccagcatc gctttgagat cgccaaaatc cccaataccc cagttttttt ccgatcgcag
    45781 cgtataaagc tgaacgcagg caccccacag cttttgttta ttcagcaacg cctgcggttc
    45841 gtaacagcgt ttcggggcga caatcaccct gcaatgcacg cgctggtctt cctgggtaaa
    45901 cgtcagcgtg tgataacctt ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt
    45961 tacatggcct ttgtactgcg ttccttcttc ggtggtcagc agccagctat attcgccgct
    46021 gccctccacc accatcggca tttttttgcc gctggtataa accatgacat tcgggactgg
    46081 cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt
    46141 ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg taattggggc taatccccgc
    46201 cgccagcgcg gcattatcca gacgtttgct ttccatcgag cttccttagc gttttgcctg
    46261 ccagatacga gcctgataat cgcgaataga gcgatccgag ctaaacatac cgcagcgggc
    46321 ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg cggtacagca catccacctg
    46381 cttttgtgcc tctacatagg ctgcgaaatc cgccatcacc agatacggat cgccgccctg
    46441 tttgccgata ctgtgcagca tctgatcgaa ggcatgctta tcgccgtcgc tgtatttgcc
    46501 gctttccagc tctttcaata ctgcgtccag caccttatct ttcttccgcc atttcaccgg
    46561 gtcgtagcct ttggccagaa ttgccttcac ttgttccacg gtatgaccaa aaataaagat
    46621 attttcttca ccaactttct cggcgatttc aacgttcgca ccatccagcg taccgacagt
    46681 aagcgcacca ttgagcgcca gtttcatatt gccggtaccg gaagcttctt tacctgccgt
    46741 cgaaatttgt tcggagatat ccgccgccgg gatcagtttt tccgccgccg aaacgcaata
    46801 atccggcagg aacaccacct tcaatttgtc gccaacctgc ggatcgttgt tgatcacgtc
    46861 agccactttg ttgatcgcaa agataatgtt tttcgccagg tagtagcccg gtgccgcttt
    46921 cgcgccaaag aggaagacgc gcggtacgcg atcagcctgc gggttttcac gaatttcttt
    46981 gtacaacgcc agaatatgca gcagattcag gtgctggcgt ttgtactcat gcagacgttt
    47041 gatctgaata tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa ctttcacaaa
    47101 ctctgccaga cggactttat tcgcctgctt gatctcgcga tattgctgac ggaatttcgc
    47161 atcatcagcg aatttttcca gattgatcag ttgatcgaga tcgttagtcc actctttttt
    47221 cagtgattta tccaacagag ccgccagtgc cgggttgcac tgtttgatcc agcgacgtgg
    47281 ggtaataccg ttggtgacgt tatggaattt gttcggccat agctggtgat attccgggaa
    47341 cagatctttc accaccagat ccgagtgcag ggccgcaaca ccgtttaccg cgaaaccgcc
    47401 aaccacacac aggttcgcca tatgcacttg tttgtcgtgt accaccgcca gtttggccca
    47461 tactttttca tcgcccggcc aggttttttc caccagcgtt ttaaagcgtg tattaatttc
    47521 gttaataatc tgcatgtggc gcggcagtaa gcctttcacc agtttcacat cccagcgttc
    47581 cagcgcttct ggcatcaagg tatggttggt gtaagcgaaa gttttactgg tgatggccca
    47641 ggcatcatcc cagctcatct ggtgctcatc gatcagcacg cgcagcagtt ctggaatcgc
    47701 gatagttggg tgggtatcgt tcagctgaat aacttcgtac tccgccagct cgtgcagttt
    47761 acgccccgcc agatgatggc gacgcaaaat atccgctacc gagcaggcac actggaagta
    47821 ttgctgcatc aggcgcagct ttttaccggc agtatggttg tcgtttggat agagaacttt
    47881 ggtcagtttt tccgcgttga tgccctgctg ttcggcacgc aggaaatcac catcgttaaa
    47941 tttagtcaga tcaaacggat gcgcatgcgt cgcctgccac agacgcagcg gctgcgccac
    48001 gccattacga tagccgacaa cggggagatc ccacgcttga ccggtaatgg taaactccgg
    48061 ctcccagcgt ccgtctttcg tcactttacc gccaatccct acctgcacat ccagtgcttc
    48121 gttgtggcgg aaccacgggt agttactgcg atgccagtca tccggcgctt caacctgttt
    48181 gccatcgaca aatgactggc ggaacaagcc atattgataa ttaaggccgt aaccagtagc
    48241 tgactgcccg acagttgcca ttgagtcgag gaagcacgcc gccagacgtc ccagaccacc
    48301 gttacccagc gccgggtcga tctcttcttc cagcaagtca gtcaggttga tgtcataagc
    48361 cttcaacgaa tcctgtacat cctgatacca gccgagattc aacagattgt tgcccgtcag
    48421 gcgaccaatc aaaaactcca ttgagatgta gttaacatgc cgctgattcg ccaccggttt
    48481 ggcaaatggc tgagcacgca gcatttcggc cagtgcttcg ctcactgcca accaccactg
    48541 acgaggggtc atttcagccg cagaatttaa gccataacgc tgccactgac gtgaaagcgc
    48601 ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata ggagttccac ttttcttaga
    48661 ttttcaacac aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg
    48721 ggggattagg cagggaggag ttgcggggat gagcaaggaa atgtgatctc gaccacttaa
    48781 agctagtgca aaccacagga ttagcagcaa atcaatgcca tacagcgcag aaaatctgta
    48841 tcaaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc ggaaggtaac tctataatta
    48901 agtaaaggag tgaaacagtt tcacaagtaa aatatccagt gtgctccatc tcattcttaa
    48961 tagatttatt aagatcatct ttttagatgg cactttcatc agaaatgaag aagaaaccct
    49021 tgcttaaatg aaactgatga acataaggaa aataccgtac aacccagtat tcacgctgga
    49081 tcagcgtcgt tttaggtgag ttgttaataa acatttggaa ttgtgacaca gtgcaaattc
    49141 agacacataa aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac cttataacca
    49201 ttaattacga agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa gtgattaact
    49261 atgctgattc cgtcaaaatt aagtcgcccg gttcgactcg accataccgt ggttcgtgag
    49321 cgcctgctgg ctaaactttc cggcgcgaac aacttccggc tggcgctgat cacaagtcct
    49381 gcgggctacg gaaagaccac gctcatttcc cagtgggcgg caggcaaaaa cgatatcggc
    49441 tggtactcgc tggatgaagg tgataaccag caagagcgtt tcgccagcta tctcattgcc
    49501 gccgtgcaac aggcaaccaa tggtcactgc gcgatatgtg agacgatggc gcaaaaacgg
    49561 caatatgcca gcctgacgtc actcttcgcc cagcttttca ttgagctggc ggaatggcat
    49621 agcccacttt atctggtcat cgatgactat catctgatca ctaatcctgt gatccacgag
    49681 tcaatgcgct tctttattcg ccatcaacca gaaaatctca cccttgtggt gttgtcacgc
    49741 aaccttccgc aactgggcat tgccaatctg cgtgttcgtg accagctgct ggaaattggc
    49801 agtcagcaac tggcatttac ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca
    49861 tcgccaattg aagccgcaga aagcagtcgg atttgcgatg acgtttccgg ttgggcgacg
    49921 gcactgcagc taatcgccct ctccgcccgg cagaatactc actcagccca taagtcggca
    49981 cgccgcctgg cgggaatcaa tgccagccat ctttcggatt atctggtcga tgaggttttg
    50041 gataacgtcg atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg
    50101 aacgatgcac tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg cctcgaagag
    50161 attgagcgtc aggggctgtt tttacagcgg atggatgata ccggcgattg gttttgctat
    50221 cacccactgt ttggtaactt cctgcgccag cgctgccagt gggaactggc ggcggagctg
    50281 ccggaaatcc accgtgccgc cgcagaaagc tggatggccc agggatttcc cagcgaagcg
    50341 attcatcatg cgctggcggc aggcgatgcg ctgatgctgc gcgatattct gcttaatcac
    50401 gcctggagtt tgttcaacca tagcgaactg tcgctgctgg aagagtcgct taaggccctg
    50461 ccgtgggaca gtttgctgga aaatccgcag ttggtgttat tgcaggcgtg gctgatgcaa
    50521 agccagcatc gctacggcga agttaacacc ctactggccc gcgctgaaca tgaaatcaag
    50581 gacattagag aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca ggtagcgatt
    50641 aacgatggta atccggatga agcggaacgg ctggcaaaac tggcactgga agagctgccg
    50701 ccgggctggt tctatagccg cattgtggca acctcggtgc tgggtgaagt gctgcactgc
    50761 aaaggcgaat tgacccgctc actggcgcta atgcagcaaa ccgaacagat ggcacgccag
    50821 cacgacgtct ggcactacgc cttatggagt ttaatccagc aaagtgaaat tctgtttgct
    50881 cagggattcc tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct gatcaacgag
    50941 cagcatctgg aacagctgcc aatgcatgag tttctggtgc gcatccgagc acaactgtta
    51001 tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat tgaagtcttg
    51061 tcgtcttatc agccacagca acagcttcag tgcctggcaa tgttgattca atgctcgctg
    51121 gcccgtggtg atttagataa cgctcgtagc cagcttaacc gtctggaaaa cctgctgggg
    51181 aatggcaaat atcacagcga ctggatctct aacgccaaca aagtccgggt gatttactgg
    51241 caaatgacca gcgataaagc cgccgctgcc aactggttgc gtcatacggc taaaccggag
    51301 tttgcgaaca accacttcct gcaaggtcaa tggcacaaca ttgcccgtgc acaaatcttg
    51361 ctgggcgagt ttgaaccggc agaaattgtt ctcgaagaac tcaatgaaaa cgcccgcagt
    51421 ctgcggttga tgagcgatct caaccgtaac ctgttgctgc ttaatcaact gtactggcag
    51481 gccggacgta aaagtgacgc ccagcgtgtg ttgctggacg cattaaaact ggcgaatcgc
    51541 accggattta tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag
    51601 cttattcaac tcaatacgct gccggagctg gaacagcatc gcgcacagcg tattctgcga
    51661 gaaatcaatc aacatcatcg gcataaattc gcccatttcg atgagaattt cgttgaacgt
    51721 ctgctaaatc atccggaagt gccagaactg atccgcacca gcccgttgac ccaacgtgaa
    51781 tggcaggtcc tggggctgat ttactccggt tacagcaacg agcaaattgc aggtgagctg
    51841 gaagtggcgg caaccaccat caagacgcat atccgcaatc tgtaccaaaa actcggtgtc
    51901 gcccaccgtc aggccgcggt acaacacgcg cagaaactgc tgaagatgat gggatatggg
    51961 gtgtgagttt agccggataa tacgtcggat ccggcttaca tctttatatc attcaatgct
    52021 cacccgcgtt acgccatccg cctccaccag actaaaccgc actggcaaga aacgctccac
    52081 caccgcaata ttggtcagca gatggcatga gggatgggcg accgtaaatt ctcccgcgcc
    52141 cgccagcgcc atcggcagta ccagctggtc ggcgagatat tcccccaccg ccgccggact
    52201 tgccaggtag cgtttcacct ctttcaccaa ctgtgccgcg accacttcgg cgctgacgcg
    52261 cttttcaccg acgacaaaaa agcgttcggt gatattttca ctttcgactt caagcgaaac
    52321 ggtattaccc ggtccctggt cacgcggcag gttatgaata ttctgctcat gcagggaaaa
    52381 actccccgcc agtgtagcga tttcacgctc agcgacatgg cgcggcacgc cagctaatag
    52441 cacctctcca cgcatctgca caatgttccc gcgctcgcca agttgcaagg tgttaaacaa
    52501 tgccaccggc gagacttccg ttgccaccac cccgcctcca gcaggataaa aaccgtggcg
    52561 taataatgtg gtttgctgat gaatgcccat tttcgccagc agcggctcca gcacccggcg
    52621 gataaaatcc gcaggcggtg ccgacgggtt atcggtgccg ccgctcactt caacacgcga
    52681 aggcccatca gcaaaccaca gcgcgggtag caccgtttgc agcaccagcg tgctgcttcc
    52741 ggcactaccg atagcaaagc ggtaatcgcc gccacgcacg gtaccgggcc ggaagaccag
    52801 acgctgtgac cccagctccg cgccttccac cgttgcccta caaatttccg cagccgcttt
    52861 taccgcggtc agatgttggc gcaacagccc cggtttcgcc cgcccggcac gaatgccggt
    52921 gatggtaaat ggctggccgg ttatcatcga caggctcagc gccgagcgca ggatctgccc
    52981 gccgccttcg ccctgtgcgc catccagcgc aatcatcctt ttcatcattt atccttttac
    53041 gcacaccacc tgacgcaggg tatagataac ttccaccaga tcgctttgtg ccgccatcac
    53101 cgcatcaata tctttatacg ccatcgggat ttcgtcgatc acttcggcat ctttacggca
    53161 ttccacatgc gcggtggcac gaatttgatc ttccacgctg aacatttttt tcgctttagt
    53221 acggctcata acccgcccgg caccgtggct gcacgaacag aacgactctt catttcccag
    53281 cccacggacg ataaagcttt tcgctcccat cgaccctgga ataattccat attgaccagc
    53341 acgcgcagac accgcgcctt tacgcgtcac gtagatctct tcaccaaagt gctgttcttt
    53401 ttgcacatag ttgtggtgac agttgatctc ttccatcgcc agcgtttgtg gctgtctgac
    53461 cgttttctgc gtaatgctct gcaacgccgt taccacgttt tccatcatcg catcgcggtt
    53521 aaggctggca aaaagctgcg cccaggccac ggctttcagg taatcatcaa agtattccgt
    53581 accttccata aagtacgcca gatcacgcga cggcaatgtt tcaagcgttt cctgcatctc
    53641 tttttgcgcc agatcgataa agtaagtccc gatggcgtta ccaattccgc gtgaaccgga
    53701 gtgcagcata atccacacct ggtccgactc atcaaggcag atttcaataa agtggttacc
    53761 cgttcccagc gttcccaggt gtttatagtt gttagtgtta aggaaacggg gatatttttg
    53821 cgttaaccac tgataaccgg cttccagctc agcccatttc gcatcgacgt taacaggcgg
    53881 attttcccat gcgcctttat cacgtttaca acgtccggta gtacgtccgt gcggcacggc
    53941 tgtttcaatc gcctgacgca gctccgccag gttttccggc aggtctgccg ccgttaacgc
    54001 ggtacgcagc gcgttcattc cacaaccgat atccacgccc accgccgccg gaataatcgc
    54061 ccctttggtc gggatcacgc taccaatggt ggaaccttta cccaggtgta catccggcat
    54121 taccgcaata tgtttgaaaa taaacggcat cttcgccgta ttaataagtt gctgacgcgc
    54181 atcggcctct accggcacgc ctttggtcca catttttacc ggggcatttt cagtggtcag
    54241 taattcgtaa ttcattttgt ttttctcttt tcgttgtttg ctgtcctgat aattgcaacc
    54301 gtcgtgccag aaaattaaat aagcagctta atttttttat ttattgtttt taaaaagatt
    54361 atatctttac gtccgtaacc ggagatttcc cgcaaagccg atttaccgat aatgaaatct
    54421 cgtcttttat aaggatatct aagatgcgta aaacagtggc ttttggcttt gtcggaacgg
    54481 tgctggatta tgccgggcgc ggcagtcagc gctggtcgaa atggcgtccg tcactctgtc
    54541 tatgccagca agaatcgtta gtcatcaatc gactggaatt gttgcacgac gcccgctcgc
    54601 gctcgctgtt tgaaacgctt aaatgcgata tcgccagcgt ttcgccagaa actgaagtgg
    54661 tgagcgttga gattgaactg cataacccgt gggatttcga agaggtttac gcctgcctgc
    54721 atgatttcgc ccgtggctac gcgtttcaac ctgataaaga agactattta attcacatca
    54781 ccactggcac ccacgtcgca cagatttgct ggtttctgct ggcggaagcg cgttacctgc
    54841 ccgcccggct gatacaatct tccccaccgc gcaaaaaaga acagccgcgc ggcgcaggtg
    54901 aagtgacgat tatcgatctc gatttaagcc gttataacgc catcgccagc cgctttgccg
    54961 aggaacggca gcaaacgctc gattttctta agtccggcat tgccacgcgt aacccccact
    55021 tcaaccgcat gattgagcag attgaaaaag tggcgattaa atcccgcgca ccgatcctgc
    55081 ttaacggccc gacaggtgcg ggcaagtcat ttctggcacg acgcatcttc gaattaaaac
    55141 aggcacggca tcagtttagc ggcgcatttg tagaaatgaa ctgcgccact ctgcgtggcg
    55201 ataccgccat gtcgacgcta tttggtcatg tgaaaggcgc gtttaccggg gcgcgggagt
    55261 cgcgggaagg attattacgc agcgccaacg gcgggatgtt atttcttgat gagattggcg
    55321 aactgggcgc agacgaacag gcaatgctgc tgaaagccat tgaagagaaa accttttatc
    55381 cgtttggcag cgatcgccag gtgagtagcg actttcagct tatcgccgga acggtgcgcg
    55441 atttgcgtca gctggttgcc gaaggcaaat tccgcgaaga tctgtacgcg cggatcaatc
    55501 tctggacctt caccttgccg gggctgagcc agcgccagga agatattgaa ccgaaccttg
    55561 attatgaagt ggagcgccac gcctcactca ctggcgacag cgtgcgtttt aacaccgaag
    55621 cgcggcgcgc ctggctggct tttgcgacct ctccccaggc aacatggcgc ggtaactttc
    55681 gcgaactttc tgccagcgtc acgcggatgg caacctttgc cactagcgga cgcatcactc
    55741 gggaagtggt ggaagatgag ataaaccgtc tgcgctataa ctggcaggag agtcgcccct
    55801 ccgcgattac ggcgttgctg ggcgcagagg cagaaaacat cgatctcttc gaccgtatgc
    55861 aactggaaca cgtaatagcc atctgccgcc aggcaaagtc actttctgct gccggtcgcc
    55921 agctttttga tgtttcgcgc cagggcaaag ccagcgtcaa cgacgcagat cggctacgca
    55981 aatacctggc gcgttttggt ctgacgtggg aagccgtgca ggatcagcac agctccagtt
    56041 gaatatgatg gtccgtcagc acctgcatga cgcttactgg cggcggggcg tcggtgtaga
    56101 cagcatcaac catgctgatg ctccccatat tgaccatcgc attacggcca aatttcgagt
    56161 gatcgacaac cagcataacg tggcgcgagt tctcaataat ggcgcgtttg gtgcgaactt
    56221 cgtggtaatc gaattccagc aacgagccgt cgctatcgat gccgctgatc cccagaatgc
    56281 cgaaatcaag gcggaactgg gagataaaat cgagcgtcgc ttcgcctatg atcccaccat
    56341 cgcggctacg taattcgcca ccggcgagaa taatgcgaaa atcctctttt accatcagcg
    56401 tgttagcgac gttaagattg ttggtgacaa tgcgcaaatt gctgtgattg agcagcgcgt
    56461 gcgccaccgc ttccggggtg gtgccgatat cgataaacag cgttgagcca ttaggaattt
    56521 gctccgccac tttgcgggcg atgcgctctt tttcttcggt ctgggtggct ttacgatcgt
    56581 gccatggcgt gttaaccgaa ctggaaggca gcgccgcacc gccatgatgg cgcaggatca
    56641 ggttttgctc tgccagctcg ttgaggtcgc gacgaatagt ctgcgggctg acggagaagt
    56701 gctccaccag ttcctcggta ctgacataac cctgctgttt aaccagttcg ataataccgt
    56761 tgtgacgttg tgtttgtttc atttataaat ccctggaatt attttcgttt tcgcgcattg
    56821 agcgaatcaa caaaagccat cgctaaaccc acggctaacc cggcgatgtg tgctccgttc
    56881 gccatcgaca tcccaaacaa atcaaaccat ccggcgacaa tccagatcag cgcaaagata
    56941 attaacccac gttgcaggta aatgccactt tgcggatcgc gttcgccacg tagccagacg
    57001 tagcccatca gcgcatacac cacgccagaa agcccgccaa accacggccc gctgaatttt
    57061 tgctgcacat agccgcttaa cagggcgcta atgagagtaa tgacaattag cttaccgcta
    57121 ccgaggcgtt tttccaccgc accgccgaga taccaccacc agagcaggtt aaagaggata
    57181 tgcatcagcg agaagtgcat taacgcgtgg gtgaagtaac gccagaactc aaatttcagt
    57241 gttggatcga atggccaggc cagccataac atcacttcct gatcgccgag aatttgcatg
    57301 gcaataaaca ccaccacgca ggcgatcatc atcacccagg ttaccggacc tgcgcgttca
    57361 cgcaaggcag caaagaaagg ataacggcga taatgcaggc cactgccggt atggcccgcc
    57421 tgccagctgg ccgccagata acgcggatct gccgggtttt cgagaaaacg cgccagctct
    57481 gcccgcacgc gctcggcctg ggactcatcc gccagccaga catcgctttg gttatgttgt
    57541 tgaatcgtga ggataacacc ctgcgtcgcc atgtaatcaa caaacgcctg cgccacgcgg
    57601 gggttagcaa aagaggtaat catcaacatc gttgctgtcg cttattccac acaaaagggg
    57661 acagtataaa gcgttacgcg ccgtacgcca cctctgcagg aaactgacgc cgccaggctt
    57721 caaagccgcc gtcaatgcta tagaccacat cgtagccctg ttgcagcaga tactgcgccg
    57781 cgcctttgct gctattgccg tgataacaca tcaccatcac cggtgtgtca aagtcgttat
    57841 cacgcataaa cgcgcccagt gtgtcgttag ttaaatggaa agcctgcacc gcatgcccca
    57901 ttgcgaaact ctgtggatcg cgaatatcga ccagcaccgc ctctttttcc tgcaacttct
    57961 ggtgcgcgac ggcaacgtta atacattcga actgctccat gcgtctctct ttctttacaa
    58021 acaagtgggc aaatttaccg cacagtttac gtcgaagcgg cagataaacg ccataatgtt
    58081 atacatatca ctctaaaatg ttttttcaat gttacctaaa gcgcgattct ttgctaatat
    58141 gttcgataac gaatatttat gagctttaac gaaagtgaat gagggcagca tggaaaccaa
    58201 agatctgatt gtgataggtg gcggcatcaa tggtgctggt atcgcggcag acgccgctgg
    58261 acgcggttta tccgtgctga tgctggaggc gcaggatctc gcttgcgcga cctcttccgc
    58321 cagttcaaaa ctcattcacg gtggcctgcg ctaccttgag cactatgaat tccgcctggt
    58381 cagcgaggcg ctggctgaac gtgaagtgct actgaaaatg gccccgcata tcgccttccc
    58441 gatgcgtttt cgcctgccgc atcgtccgca tctgcgcccg gcgtggatga ttcgcattgg
    58501 tctgtttatg tacgatcatc tgggtaaacg caccagcctg ccgggatcaa ccggtttgcg
    58561 ttttggcgca aattcagtgt taaaaccgga aattaagcgc ggattcgaat attccgactg
    58621 ttgggtagac gacgcccgtc tggtactcgc caacgcccag atggtagtgc gtaaaggcgg
    58681 tgaagtgctt actcgtactc gtgccacttc tgctcgccgc gaaaacggcc tgtggattgt
    58741 ggaagcggaa gatatcgata ccggcaaaaa atatagctgg caagcgcgcg gcctcgtgaa
    58801 cgccaccggc ccgtgggtaa agcagttctt cgacgacggg atgcatctgc cttcgcctta
    58861 tggcattcgc ctgatcaaag gcagccatat tgtcgtgccg cgcgttcata cacaaaagca
    58921 ggcctacatt ctgcaaaacg aagataaacg tattgtcttc gtgatcccgt ggatggacga
    58981 gttttccatc atcggcacca ccgatgtgga atacaaaggt gatccaaaag cagtgaagat
    59041 tgaagagagt gaaatcaatt acctgctgaa agtgtataac acgcacttta aaaagcagtt
    59101 aagccgggac gatatcgtct ggacttactc cggcgtgcgt ccgctgtgtg atgatgagtc
    59161 cgattcgccg caggctatca ctcgtgatta cacccttgat atccatgatg aaaacggcaa
    59221 agcgccgctg ctgtcggtat tcggcggtaa gctgaccacc taccgcaaac tggcggagca
    59281 cgcgctggaa aaactgacgc catattatca gggcattggt ccggcgtgga cgaaagagag
    59341 cgtgctgccg ggtggtgcca ttgaaggcga ccgcgacgat tatgccgctc gcctgcgccg
    59401 ccgctatccg ttcctgactg aatcgctggc acgccattac gcgcgcacgt acggcagcaa
    59461 cagcgagttg ctgctcggca atgcggggac ggtaagcgat ctcggggaag atttcggtca
    59521 tgagttctac gaagcggagc tgaaatacct ggtggatcac gaatgggtcc gccgcgccga
    59581 cgatgccctg tggcgtcgca caaaacaagg catgtggctg aatgcggaac aacaatctcg
    59641 tgtgagtcag tggctggtgg agtatacgca gcagaagttg tcgctggcgt cgtaaggatg
    59701 atgcccggtt gtgaagtgca accgggctat ttggattcct tagactattc attgtaaata
    59761 attgaataac ggcccctgag ctaaaatcgc caaacttaag cacgttcccc actcaaccca
    59821 cttctgcccc tggcttctat ttttgctacg ccactggcag taatatccca acgcaaaacc
    59881 aagctgtgca ctaatcgggc cgacaaaatc agtccacggc tgcggccaaa aaggggaaaa
    59941 attcagggga agccagacaa acgtcagaaa gaagtttcgg atattttcca gcaacgctga
    60001 tttctctgga gtaatataaa gaatgaaatt cgagagaatt aaccagaaat aggccggaga
    60061 aaagaccagc cagtaatgca gtctgttttt taattcagga ggcgttctgc gggagaattg
    60121 ccaaccgatg ccacccatta cactgaaaaa aagtgcatat tcaaccatta actcaagttg
    60181 taattcctga agagaaatac gcacaaccag gagcggaaag gagaatatta tttcagccag
    60241 aaaagttaac gtgaatataa gtacggctaa ggcaataacc gtcggccata ccaataatgg
    60301 cacgataaca aaaccgagaa ttacaggggg aatataaagc cggaatttaa tcatttttat
    60361 tttcctggtg attcggatga tgcgtcatac atcgcgcata aacactcatc aatcatccag
    60421 tcgtcttccc agacctgata atgtgtgccc tgttcgggct gattaagcag gttctcaatc
    60481 gcctgccgcg cacgttcttt caggatgaat tgattgttga aggcccaggc attgatttgg
    60541 gcggtaatgg ctttatcgtc atcatcctgc tcaaaacgac tattctgaat attctcttcc
    60601 ctgttatgcc acagcaaaca tgccatcgat actgtgggtt ggtaaccggg tacagagatg
    60661 atggcatcaa gcaatggcga aagagcgata tcggcacggg aaaacatttc cagcatatac
    60721 tgtaattcga cagcctcatc gccagatacc caatcagcca tatactggca ggcaaatcgc
    60781 tgcaatattg cccgttcatc ctcacgccag ttttctttac tggcaaggtt gatacgccgt
    60841 agagaaaata cttcgtgaat attgtcaaat tgcccctgcg caacaaactc cagtaaacga
    60901 ggaagaaaat aacgaacctc atcgctgttt tgcggatcag gtcctggact actattccag
    60961 gcattgatta aggaataagg aatcgctcgc aacgatgtat tccgtaatgc tttttgttcc
    61021 tgttgactca ggcaatattc acaacaaacc acaaattgct gcggcaccgg ataacgacga
    61081 aaaatatgat aaacctgctc aattaatgcc tgcatggcgg agctgggttg cataattacc
    61141 ccctcttccg ctggtcgcgt gtaaacggtt gataaacaaa acgagcattc gccgcattca
    61201 ggcgaataat ggaactacga gagagggaaa agctgggccg aatgcaataa ttacaatccc
    61261 gacagctttc ctctgtgcaa ttttccactg agtaatgacg cgcgtagagt ttcagttcag
    61321 cctgcttatc ccgaaaaccg cgtaagctgt tatcggtatg gaatttccat gcttctttaa
    61381 acacctgaaa gatataaaca tcctcaatcc aggagtcaca ttttttaagc gggcgctttg
    61441 ctatgatcac ccgctcatcc ggattgagca ttgccgtaaa atgaacggta aagtcatttg
    61501 tcgtattcag gtcacattga acatctggta catcactgat ataggggtca aatccaaaat
    61561 cagcaccaca ggcacaaaga cgaccgatcg tggcggtatc gagggaaaat cccggccata
    61621 cattgctgga ggttaagcca atatttaatg tgacatcata tcgagataat ccctcaagac
    61681 cgacagtacc ggcgaacttt tccccttcat ccaggacctg gttaatgagt tgctgaataa
    61741 tgccttcatc ccagtatgtt gatttgcagt tggcaatcgt aatatccacg ccagtgaatg
    61801 gatcagtacc ggagaggctt gcaaataacg tatcaactaa ttccattttt attatccggc
    61861 ttaatgcaac attttatcca tcggcacata acctacattt tccaccagat tttgtccctg
    61921 tagaattaaa aatatttttt tgttttacat tctgttcatc cctattattg cattttattt
    61981 tgcggtgatg gctaaaccga ttctgtacta gctcaaaaat tactgatatt ccactcgcaa
    62041 tacagccagt gcattataac gacctgccgc aggcactttt ccagttgtac taacaggtaa
    62101 agccaggaaa gtaacatcag cagtttgcgc agaagcagcc tggttttcca ccaatctgaa
    62161 cggaacgcct actgatgttg ccagagcatt taaatctacg gagttttgct gactatccat
    62221 aatcataata ccaacatcag gattatcttg tgcctgaata atactttgtt tcccattggt
    62281 atcacgtgta gctgaaatgt taccgtcgaa taccaacgtc attaccgcat ctgtatcttt
    62341 attcgtacaa ttaatcggaa tagttgttgc tacaggagta acaccagcag gcttcgctcc
    62401 agcaccagcc cggctcaatt cactggccca cacatccggc atattaacag taaaactagt
    62461 accctcatta atagtgcaac cttgcggaac ctgaatatcc aggttactga tccaggcatt
    62521 aaataatggt actggcccat gttccgtttg ccgctgactg gcatacaatg aaactaaaag
    62581 tacattattc agagagacat cagagatgat ttttttatcc aacctaattt taatatagcc
    62641 ttctgttcct gaagaccagt aatctgcttt acttctgtca gcaggataat tagtactata
    62701 gttctcgaat ggtactgttt tatctgtcat cgtcccattt tgattgtaga gttttatatg
    62761 tgttgaaata gatatttcat gcgtaacagg aataaatatt tctgctccct gtggattacc
    62821 tgaagcacct gaaggatcta ttcctgaaat acctgaaaaa taaacttcat aatcatcaag
    62881 agaattacag tatgctttat atcgacctat tttccagttt tgagatggta aaacagtacc
    62941 ggctgaattg ttgttaacat ctgaaataat aactgtgcca aaatcataaa aaaaggtcgc
    63001 tggcgtagta ttgtttcctg gagtaccagg atagcaaaac tcacgatccc ggcccatttc
    63061 agcataggct acgacatggt accctaagaa taaaaaaatt ataaaaataa ttttcacttt
    63121 atatccggca taagtaatca tacgatttcc ttttactaat ccgttttaaa ttgtgagcct
    63181 gacacatcca tttgcaacgg attcatattt actttccata caggcaatac attattataa
    63241 atgtccaatg tctgccatgt tatttcacaa atcccttgcg ctttacacca tgattgttga
    63301 cgatggctaa atggtgcgat cataccacca tccataactt taccatcaac atgaaggtta
    63361 tagagtgtca catatctggg gcctggatta taaacggtaa caatttcatt ttttcgtgcc
    63421 cagcgtaatt tttgatatga aatattcggg tcttcagaca aatttgatgg ccgatagaat
    63481 attttcatac gattgcggac cgctatttgc aattgattat cagttttggt tggatgacta
    63541 ttttccaacg atggtaatgc agcaacatta agtataaata aactttcccg gtcggcaggt
    63601 aaactgccac caacacgatt aattcttatt tttgtatttt tgtcaggctt caaaacaaac
    63661 agtggcggag ttgcgataaa taccgttcca gaaaatgagg gcaaagattc aaccgttccc
    63721 gtcaactgac gagcatcatc aggggagact gcaaccttga ctaacatatc tctgtctgaa
    63781 gtgtttttga gttcaacaga tattgaggac tgattttccg gatacgcaaa acgcgttcca
    63841 ccgatcacta atcctgcttg tgctgtagag aataaaaggg aaaataatac tatgactacc
    63901 ttaatcatag gtgacaataa acgatactga cgcattggca tcccctggtt ggacattatt
    63961 ttttaatgct ttgtaagccg caaaaaaatg caatatatta tcaactgatg atgtaagtga
    64021 aaaacctatg cttttaacat cattactaat actgcctttg ttcttatcaa aaatcgtgag
    64081 tgcaagccca tctaccgagc catcttccag cttatataaa ctggcatctt cagaaactgt
    64141 attgccggta aactttatat acgcgttgcc aacatcacta ccacagcggg ttaacgttat
    64201 ggaaaaataa accttttcag taacatcccc tgcactcctt accgaactac tgggtacgtc
    64261 tggcatatta actgtaaaat tttttgactc atcagaaata atgcaggttt tttcggtaat
    64321 tagcccttca atacgaacca ctccggcatg agtgatatca tcagcaaaag aatttgttga
    64381 tattataaac aaaaataaac taatgaatat cttcatatct ccctcactga taatcgatat
    64441 caaaatacaa cagagcattt gcttgcccgg catgtacatt ttcctgagtt gatttatagc
    64501 gaagtttaaa cgtcaaatta gcatcaccgt tttcatttaa cactaaatca cgaaaacctg
    64561 attcaccatt aatagcaatc gccacatcat tgttatccaa tatttctatt cccagtccag
    64621 tcgcactatc tgcaccggcg gagagacgca gtaatgcgct attaatggca tctgcatcac
    64681 cagtaaaccg cacattggcg ctcacaccag aactataaac agtacttttt gcagtcgggc
    64741 atccttgtac attaatcgta aacgtttgac tcggagaaac atcacctaca tttaaaaatt
    64801 cattagcatc ataagtgccc atttttaccg actgatcttt acttccggta gtaatgtcac
    64861 agctttttgc caacaaaatc ccagtaaatg taacaacaac tgaatcattg gcctgccctg
    64921 agagcggaaa agcaaaaaaa gcgaaaagaa taaaaaatac agaatattta gagaatatta
    64981 aattcattag cggcactcca gagtagcata gctaacagga ttattcagtt ctgtttcagg
    65041 gagcttgtaa ttagcctgac atttttgctg aacaccatct ccccactgga tattaataac
    65101 accttctaaa ggcaatcctg agagataaag ttgtccatta tcgccgacaa tcccggtaat
    65161 ttgactacta tttttaatct gtgcgctggc tgtagcaccg aatgggactg atttattacg
    65221 ataacgaact gtgaacaaac cacgaatacc gatatgagca gcaaactctg cacgaactaa
    65281 cgcacctttt cccggaataa cttcagtgga agtgatatcc agttcagtat gcatattcat
    65341 tgacatagga tctaatgaaa tattattacg tctatattct gttgcataag gaacaactgc
    65401 atatccacgc cagtcagtgg atattcctgt cccccgctgg acatccacat tattcgctcc
    65461 aggcgctttt atcaaaacat tagtttcacc taatggttga ctcaacgtaa gaccattttc
    65521 atgtacaaca agagcaccac tgatgccata gtttatctgt tgattacctt cattatcgta
    65581 actgtaggcg gaattcaagg aaccatatgc cccacgataa ttaacaccaa cagaaccact
    65641 actgctatta tcctgattag caaaaccatg ttgtatgtta taactgagat tatgctgctc
    65701 caacagagaa ccagataccc ccgtatattg gtttacctgt ccgtggttat cctgactata
    65761 accatacgtt gcagtcatgg cattcgatga tgagcgccca tcattcgtgg aagataacca
    65821 attacttata ggtaatgaga catttagcga gacaatttta tccgcatttg tccctggtga
    65881 tttactgtaa ttccacgcaa catttagata gataccctta atactggtgt tataaccaac
    65941 ttggtataaa gaatcttttt tatcagtatt ccagtatgtt tgctgactag ccgataaata
    66001 catagacccg tagttaccga aaggttgcga tacactagcc tgaaattttc cacgtttgct
    66061 ataacgcaag ttataaaaat cattccagtt ataaacgtaa tctttatcat ctaacgtttt
    66121 tggatcaaca acagtccctg acatttgttg gtatgtcgta tcacttaaag tgtaaaagcc
    66181 ctgggttgaa taacgatagc caataatatg gaatgctgtt cctgtctgaa ctaacgattt
    66241 ggaataaaga aaacgataag attgcccttg atgtttgctc ccatccacaa gcgaactgtt
    66301 agcctgagta acatcaaacg atgttgcacc aaatacaccc agattcaggc caaggccgag
    66361 cgccaacgcc cggtaatcct cagagaattg tgccccacca taagccgtaa tatcccaggg
    66421 taaaccccaa attaattccc cctgaaatac ataaggactt ttctggttat agctattgct
    66481 tcgatattgg ccaaccgtag tagaatattt tgaatgacct tctcgttgca ggatggggac
    66541 agcggcataa ggtacggtat agacagtttc tgaattatca gactctttga tggtaacttc
    66601 cagatctccc gcagatgatg tcgggttaag atcgctaatc tcaaacggtc ccggcggcat
    66661 ataggtttga taaatgacat aaccattctg tttgattgtt acctgagcgc gacttttcgc
    66721 aattccgcgc accgtgggag cgaaaccact ttgactatcg ggcaacatat tttcgtcaga
    66781 ttccagtttt atgccacgaa tactaacact gtcgaaaaca tctgatgatg accatgtatc
    66841 tcctagcgtt aattcacttc tgaaaggaat aataacgcgc tgcaacgtac tactgatatg
    66901 ctccagttta cctgctgaat tactcccacg actccaggtt gaataatcgc gaaaacgcca
    66961 tgcaccaata ttgatcccag cgcgaagatt taaataatag ttatttgaag agtaatcacc
    67021 acgatcgtta ttaccagaaa agtcataatt cagcaaaaaa gctgtaatac cgttatccca
    67081 ctgttctggt ggaatatatc cgcgggcgtc acgttttatt atcgcctgcg gaaaggtcag
    67141 tttaagtctc tgtgcatcca gctccggcat gtaatgcaca tcaggaaacc agatacgcaa
    67201 atcaaggcaa ctattttcag ataaagccaa agcctgctct ggcagcgcat tcttgttaat
    67261 tccaaggctt aataagtcag caagtgaaag gcagggaatc agttgattct catcgtctgc
    67321 tttaaaagtt acagtacgag agagtaaagg ggaatcattg acccaaactt cgacttcata
    67381 gctaccagga agataagtca tccctttctc gaaacgggat aagtcagcaa cagcttcagt
    67441 atcacccgac agaaaagcag ggttaaagcg cattcccgcg attgaacatg caggcataat
    67501 aaccagaaga agcggtacaa cccgtttcat ctgcttttgt gccgccatcc aatattcgca
    67561 tcttttgagc tttaggttca tatccctgac ctgtgttgca tctgaactat cccgttgtta
    67621 ctgcattatt cctttctgcc gctccgtcgt agcgccaaag tcattaattg tttgataagt
    67681 aatttcacca gccaacgtca ctgccgacga gaccgtaata tcgcttttcg gtggtacaaa
    67741 cgtattgggc aatttactcc cttgtacttt aattcctgtc agtgtcaggt aataaggcga
    67801 aggattgata acttttagtt gtttgccctc atacttaaac cgcaacatat ttcgtgcttt
    67861 tcctggttgc acaggtagat tttccgggcg ataaaatagc ttgattttgt tttgtaatgc
    67921 caactgtaag gtattcttct gtgtattttt ctcatctgtt ggcggaattg ctttaacatt
    67981 catccaatac aaagtttcac gatcggtcgg caaattagtt ttaccaagaa acatgatacg
    68041 taatgaagct tctttacgcg ttggcaacat aaagagggga ggagtgataa caaaatcctg
    68101 gctctttttt tccgttttac tgtcatcaac ccatgattga attaaataac tggtgttgtc
    68161 accgctattt tgcacactga gtgttatttg cttactttct gccggataaa tcaatcgcgt
    68221 tacgccaagg ccaaacccgc ccgcggacgc attattaata aataatatcg atagtaaaaa
    68281 aagaatccct gagcgtataa tattttgtga tacttttaat tcatccctga atccataaac
    68341 ccatttttta tttccaataa tacatttcat acagcatcct tgttgatgaa ataacacctg
    68401 ttattcatac tctattaaat aatttcaatc tcaggttaca actttttgta acctgaaatc
    68461 gtatttatat ttactcgtac agcaccacaa aacttgctgt tgacttaact acaccaggtg
    68521 caacagtgct accgttttga gcataacctg cagtaaaggc cgcgctgaaa tcatgggtcg
    68581 tagcatttcc tggaattgta taagcggcgg tggaggcgct accctctgcc gcagtagtat
    68641 tcaggttaat cagaccatta ttcgtcgtac ctttggagta aattcgaatc cctacaccag
    68701 tcgcccccgc attgccgctg ttgtcagcat ccgttgatcc ttctacttcg ttagcgagcg
    68761 ttttattagc gctatctgcg gtaccggtaa agcggaactc aattttctga tcctctgtat
    68821 tgctacattc ggtcagatta atattgaaat cggtctggtt gctcgccccc tgtgcttcta
    68881 tgtcgatacc agtgaaaagg ttggaaggca caaccggcag gacaacttca atctgttttg
    68941 aatcggttgc gaccttacag gtagctttca ctacacggcc agtaaagcgg attttccccc
    69001 ccattgaatc aacgttgtca tccatatcag tgagcctggg ataatcaacg gtgctccagt
    69061 cagtatccgc tccaccaaat tgtgctgcat cgccgggtgc tgcggttacc gcagtagaca
    69121 tcagcatacc tgatgccagt aatactgcgg ataaattaga taaattgaat ttcatttata
    69181 cctttccttt tgttaaaaaa tccaagcaac attggtgttt ttacattctg cgttaataag
    69241 ttatatc
//
LOCUS       DAZKJR010000006        65866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKJR010000006 DAZKJR010000000
VERSION     DAZKJR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.006"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..478)
                     /gene="folK"
                     /locus_tag="ACHOWS_000395"
     CDS             complement(<1..478)
                     /gene="folK"
                     /locus_tag="ACHOWS_000395"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFQ0046038.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELLFPDGLSLVEALQAKGFNELDKW"
     gene            complement(475..1872)
                     /gene="pcnB"
                     /locus_tag="ACHOWS_000396"
     CDS             complement(475..1872)
                     /gene="pcnB"
                     /locus_tag="ACHOWS_000396"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFQ0046039.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1932..2828)
                     /gene="gluQRS"
                     /locus_tag="ACHOWS_000397"
     CDS             complement(1932..2828)
                     /gene="gluQRS"
                     /locus_tag="ACHOWS_000397"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFQ0046040.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTLFTDQLRGIIHANEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNTQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2895..3350)
                     /gene="dksA"
                     /locus_tag="ACHOWS_000398"
     CDS             complement(2895..3350)
                     /gene="dksA"
                     /locus_tag="ACHOWS_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFQ0046041.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3528..4232)
                     /gene="sfsA"
                     /locus_tag="ACHOWS_000399"
     CDS             complement(3528..4232)
                     /gene="sfsA"
                     /locus_tag="ACHOWS_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0046042.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTSC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4247..4777)
                     /gene="thpR"
                     /locus_tag="ACHOWS_000400"
     CDS             complement(4247..4777)
                     /gene="thpR"
                     /locus_tag="ACHOWS_000400"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFQ0046043.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4851..7280
                     /gene="hrpB"
                     /locus_tag="ACHOWS_000401"
     CDS             4851..7280
                     /gene="hrpB"
                     /locus_tag="ACHOWS_000401"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFQ0046044.1"
                     /translation="MSSLPVAAVLPELLAALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVAHFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDTAQMSWLDQPPTVNLQAAKRL
                     LQMLGALEGERLSAQGQKMAVLGSDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIACRRGQDG
                     RYQLANGMGAMLDADDALSRYEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLFAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDKPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7374..9905
                     /gene="mrcB"
                     /locus_tag="ACHOWS_000402"
     CDS             7374..9905
                     /gene="mrcB"
                     /locus_tag="ACHOWS_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFQ0046045.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEDEEP
                     MPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLP
                     AAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF
                     PDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRS
                     GFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLF
                     LSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYF
                     GRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDM
                     LSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAE
                     KAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIG
                     SLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVD
                     ALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAF
                     QTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTG
                     RQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMS
                     IYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEM
                     QQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10125..12314
                     /gene="fhuA"
                     /locus_tag="ACHOWS_000403"
                     /pseudo
     CDS             10125..12314
                     /gene="fhuA"
                     /locus_tag="ACHOWS_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
     gene            12365..13162
                     /gene="fhuC"
                     /locus_tag="ACHOWS_000404"
     CDS             12365..13162
                     /gene="fhuC"
                     /locus_tag="ACHOWS_000404"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFQ0046046.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13162..14052
                     /gene="fhuD"
                     /locus_tag="ACHOWS_000405"
     CDS             13162..14052
                     /gene="fhuD"
                     /locus_tag="ACHOWS_000405"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFQ0046047.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAIIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14049..16031
                     /gene="fhuB"
                     /locus_tag="ACHOWS_000406"
     CDS             14049..16031
                     /gene="fhuB"
                     /locus_tag="ACHOWS_000406"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFQ0046048.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAINPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16066..17346)
                     /gene="hemL"
                     /locus_tag="ACHOWS_000407"
     CDS             complement(16066..17346)
                     /gene="hemL"
                     /locus_tag="ACHOWS_000407"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFQ0046049.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPHEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17512..17559
                     /gene="yadX"
                     /locus_tag="ACHOWS_000408"
     CDS             17512..17559
                     /gene="yadX"
                     /locus_tag="ACHOWS_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFQ0046050.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17571..18992
                     /gene="clcA"
                     /locus_tag="ACHOWS_000409"
     CDS             17571..18992
                     /gene="clcA"
                     /locus_tag="ACHOWS_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFQ0046051.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19016..19081
                     /gene="yadW"
                     /locus_tag="ACHOWS_000410"
     CDS             19016..19081
                     /gene="yadW"
                     /locus_tag="ACHOWS_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFQ0046052.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19074..19418
                     /gene="erpA"
                     /locus_tag="ACHOWS_000411"
     CDS             19074..19418
                     /gene="erpA"
                     /locus_tag="ACHOWS_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFQ0046053.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19465..20088)
                     /gene="yadS"
                     /locus_tag="ACHOWS_000412"
     CDS             complement(19465..20088)
                     /gene="yadS"
                     /locus_tag="ACHOWS_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFQ0046054.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20126..20926)
                     /gene="btuF"
                     /locus_tag="ACHOWS_000413"
     CDS             complement(20126..20926)
                     /gene="btuF"
                     /locus_tag="ACHOWS_000413"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFQ0046055.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20919..21617)
                     /gene="mtnN"
                     /locus_tag="ACHOWS_000414"
     CDS             complement(20919..21617)
                     /gene="mtnN"
                     /locus_tag="ACHOWS_000414"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0046056.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21701..23218
                     /gene="dgt"
                     /locus_tag="ACHOWS_000415"
     CDS             21701..23218
                     /gene="dgt"
                     /locus_tag="ACHOWS_000415"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFQ0046057.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLYSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23348..24772
                     /gene="degP"
                     /locus_tag="ACHOWS_000416"
     CDS             23348..24772
                     /gene="degP"
                     /locus_tag="ACHOWS_000416"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFQ0046058.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24927..26084
                     /gene="cdaR"
                     /locus_tag="ACHOWS_000417"
     CDS             24927..26084
                     /gene="cdaR"
                     /locus_tag="ACHOWS_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFQ0046059.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEQR"
     gene            complement(26199..26585)
                     /gene="yaeH"
                     /locus_tag="ACHOWS_000418"
     CDS             complement(26199..26585)
                     /gene="yaeH"
                     /locus_tag="ACHOWS_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFQ0046060.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGIGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26743..27555)
                     /gene="yaeI"
                     /locus_tag="ACHOWS_000419"
     CDS             complement(26743..27555)
                     /gene="yaeI"
                     /locus_tag="ACHOWS_000419"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFQ0046061.1"
                     /translation="MITRRRFLQAAAATLAAGSSLSYMHYWEPGWLELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGVAQKPDLILLGGDYVLFDMQLNFSAFS
                     DVLSSLAECAPTFACYGNHDRPVGTEKNRLIGEALKSAGITVLFNEAMLITTQKQQFE
                     LVGTGDLWTGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIP
                     LVGEPFAPIEDKRYAAGLNAFGERQIYTTRGVGSLYGLRLNCRPEVTILELV"
     gene            complement(27608..28432)
                     /gene="dapD"
                     /locus_tag="ACHOWS_000420"
     CDS             complement(27608..28432)
                     /gene="dapD"
                     /locus_tag="ACHOWS_000420"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFQ0046062.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28463..31135)
                     /gene="glnD"
                     /locus_tag="ACHOWS_000421"
     CDS             complement(28463..31135)
                     /gene="glnD"
                     /locus_tag="ACHOWS_000421"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFQ0046063.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31197..31991)
                     /gene="map"
                     /locus_tag="ACHOWS_000422"
     CDS             complement(31197..31991)
                     /gene="map"
                     /locus_tag="ACHOWS_000422"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0046064.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32359..33084
                     /gene="rpsB"
                     /locus_tag="ACHOWS_000423"
     CDS             32359..33084
                     /gene="rpsB"
                     /locus_tag="ACHOWS_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0046065.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33342..34193
                     /gene="tsf"
                     /locus_tag="ACHOWS_000424"
     CDS             33342..34193
                     /gene="tsf"
                     /locus_tag="ACHOWS_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0046066.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34340..35065
                     /gene="pyrH"
                     /locus_tag="ACHOWS_000425"
     CDS             34340..35065
                     /gene="pyrH"
                     /locus_tag="ACHOWS_000425"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0046067.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35357..35914
                     /gene="frr"
                     /locus_tag="ACHOWS_000426"
     CDS             35357..35914
                     /gene="frr"
                     /locus_tag="ACHOWS_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0046068.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36006..37202
                     /gene="ispC"
                     /locus_tag="ACHOWS_000427"
     CDS             36006..37202
                     /gene="ispC"
                     /locus_tag="ACHOWS_000427"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFQ0046069.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37391..38149
                     /gene="ispU"
                     /locus_tag="ACHOWS_000428"
                     /gene_synonym="uppS"
     CDS             37391..38149
                     /gene="ispU"
                     /locus_tag="ACHOWS_000428"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFQ0046070.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38162..39019
                     /gene="cdsA"
                     /locus_tag="ACHOWS_000429"
     CDS             38162..39019
                     /gene="cdsA"
                     /locus_tag="ACHOWS_000429"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0046071.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39031..40383
                     /gene="rseP"
                     /locus_tag="ACHOWS_000430"
     CDS             39031..40383
                     /gene="rseP"
                     /locus_tag="ACHOWS_000430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFQ0046072.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40413..42845
                     /gene="bamA"
                     /locus_tag="ACHOWS_000431"
     CDS             40413..42845
                     /gene="bamA"
                     /locus_tag="ACHOWS_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFQ0046073.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42967..43452
                     /gene="skp"
                     /locus_tag="ACHOWS_000432"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42967..43452
                     /gene="skp"
                     /locus_tag="ACHOWS_000432"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFQ0046074.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43456..44481
                     /gene="lpxD"
                     /locus_tag="ACHOWS_000433"
     CDS             43456..44481
                     /gene="lpxD"
                     /locus_tag="ACHOWS_000433"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFQ0046075.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44586..45041
                     /gene="fabZ"
                     /locus_tag="ACHOWS_000434"
     CDS             44586..45041
                     /gene="fabZ"
                     /locus_tag="ACHOWS_000434"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0046076.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45045..45833
                     /gene="lpxA"
                     /locus_tag="ACHOWS_000435"
     CDS             45045..45833
                     /gene="lpxA"
                     /locus_tag="ACHOWS_000435"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFQ0046077.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45833..46981
                     /gene="lpxB"
                     /locus_tag="ACHOWS_000436"
     CDS             45833..46981
                     /gene="lpxB"
                     /locus_tag="ACHOWS_000436"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFQ0046078.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTTRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46978..47574
                     /gene="rnhB"
                     /locus_tag="ACHOWS_000437"
     CDS             46978..47574
                     /gene="rnhB"
                     /locus_tag="ACHOWS_000437"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0046079.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRVLGLAS"
     gene            47611..51093
                     /gene="dnaE"
                     /locus_tag="ACHOWS_000438"
     CDS             47611..51093
                     /gene="dnaE"
                     /locus_tag="ACHOWS_000438"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0046080.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51106..52065
                     /gene="accA"
                     /locus_tag="ACHOWS_000439"
     CDS             51106..52065
                     /gene="accA"
                     /locus_tag="ACHOWS_000439"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0046081.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52164..54304
                     /gene="ldcC"
                     /locus_tag="ACHOWS_000440"
                     /pseudo
     CDS             52164..54304
                     /gene="ldcC"
                     /locus_tag="ACHOWS_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            54361..54750
                     /gene="yaeR"
                     /locus_tag="ACHOWS_000441"
     CDS             54361..54750
                     /gene="yaeR"
                     /locus_tag="ACHOWS_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0046082.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54815..56113
                     /gene="tilS"
                     /locus_tag="ACHOWS_000442"
     CDS             54815..56113
                     /gene="tilS"
                     /locus_tag="ACHOWS_000442"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0046083.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRMENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56162..56422)
                     /gene="rof"
                     /locus_tag="ACHOWS_000443"
     CDS             complement(56162..56422)
                     /gene="rof"
                     /locus_tag="ACHOWS_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFQ0046084.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56409..56609)
                     /gene="yaeP"
                     /locus_tag="ACHOWS_000444"
     CDS             complement(56409..56609)
                     /gene="yaeP"
                     /locus_tag="ACHOWS_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFQ0046085.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56775..57320
                     /gene="yaeQ"
                     /locus_tag="ACHOWS_000445"
     CDS             56775..57320
                     /gene="yaeQ"
                     /locus_tag="ACHOWS_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFQ0046086.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFSNLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57317..57739
                     /gene="arfB"
                     /locus_tag="ACHOWS_000446"
     CDS             57317..57739
                     /gene="arfB"
                     /locus_tag="ACHOWS_000446"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFQ0046087.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57753..58463
                     /gene="nlpE"
                     /locus_tag="ACHOWS_000447"
                     /gene_synonym="cutF"
     CDS             57753..58463
                     /gene="nlpE"
                     /locus_tag="ACHOWS_000447"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFQ0046088.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58493..59317)
                     /gene="yaeF"
                     /locus_tag="ACHOWS_000448"
     CDS             complement(58493..59317)
                     /gene="yaeF"
                     /locus_tag="ACHOWS_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFQ0046089.1"
                     /translation="MDKPKAHCRLLLPCFLFLSACTVDISQPDSSATAVDVEAKTWVV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQVCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(59370..61088)
                     /gene="proS"
                     /locus_tag="ACHOWS_000449"
     CDS             complement(59370..61088)
                     /gene="proS"
                     /locus_tag="ACHOWS_000449"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0046090.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61199..61906)
                     /gene="tsaA"
                     /locus_tag="ACHOWS_000450"
     CDS             complement(61199..61906)
                     /gene="tsaA"
                     /locus_tag="ACHOWS_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFQ0046091.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61903..62307)
                     /gene="rcsF"
                     /locus_tag="ACHOWS_000451"
     CDS             complement(61903..62307)
                     /gene="rcsF"
                     /locus_tag="ACHOWS_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFQ0046092.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAESAKPKVP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62425..63240)
                     /gene="metQ"
                     /locus_tag="ACHOWS_000452"
     CDS             complement(62425..63240)
                     /gene="metQ"
                     /locus_tag="ACHOWS_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFQ0046093.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63280..63933)
                     /gene="metI"
                     /locus_tag="ACHOWS_000453"
     CDS             complement(63280..63933)
                     /gene="metI"
                     /locus_tag="ACHOWS_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFQ0046094.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63926..64957)
                     /gene="metN"
                     /locus_tag="ACHOWS_000454"
     CDS             complement(63926..64957)
                     /gene="metN"
                     /locus_tag="ACHOWS_000454"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFQ0046095.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            65145..65717
                     /gene="gmhB"
                     /locus_tag="ACHOWS_000455"
     CDS             65145..65717
                     /gene="gmhB"
                     /locus_tag="ACHOWS_000455"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFQ0046096.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15656 a        16304 c        18078 g        15828 t
ORIGIN      
        1 accacttatc taattcatta aagccttttg cctgtagagc ttcaactaat gataagccat
       61 caggaaatag caattccggc gcaatttcaa acagtggcca cagcataaag ccgcgatttt
      121 tcatatcgta gtgcggaacg gtcagacgtt cggtatttat tacttcatta ccaaacagca
      181 tgatgtcgag atccagcgtg cgcggtcccc agcgttcagc tttacggacg cgaccttgct
      241 gcaattcgat acgctgtgtg tgattgagta gctcttcagg ggcaagagtg gttttcagcg
      301 cgacggctgc atttaagtaa tcgggttgat cttgcggccc cagcggtggg gtgcggtaaa
      361 acgaagaaac agcaagaatg cggctttctg gaatatcgcc taatgctttc agggcagcat
      421 tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc actgtcatgc
      481 ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacggcgac gcggtgacgg
      541 ttcttcatcc agttcgttaa gcatcccttt ttggtctggt ggcgcggaaa cctggaactc
      601 accccaccat ttcaccagac gttgcagttc agcgttacgc tcaacttcgg ctcgtaaggc
      661 caacaggtcg tatgccgcac ggaacttagg atgctccagc agtttccagg cgcgtttacc
      721 ctgacgtcgg gacatacgca actgcaactg ccagatatcg cgggttaacg tcgtcagacg
      781 tttcgggatc gccagtgaac gacaggcttc gtccagcacg tcgttcatcg ccagcgcgaa
      841 agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg
      901 gtaccagaac atggcggcaa acaggaacgc cgggttcacg cgcatatcgt tatggatacg
      961 cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt cgccattttc
     1021 cgtgaagtag cgggtaatgg tcgggaacag cggctggaac aggtgatatt cacacagcag
     1081 cttataggtt tcgtaaccgt agcccgcttg cagcagtttc aaagattctt caaacagacg
     1141 tgccggtgga atatcgttca gcagggtagc caggcgaggg atcggttctg cggtttcagg
     1201 actgatacgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatcc gtaccggatc
     1261 ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct tcagatcctt
     1321 catgccgcca acgtaatcac ggacggtaaa atctgccacg ctgtaataca ggctgttgat
     1381 agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa
     1441 catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt cgtgatgtcc
     1501 acggaaggtc gcaacttcga taatctccgg gccaaacatc acatgagcca gacggaaacg
     1561 gcgacccacc aggcggcagt tacggaacag tttacgtacc tgttcaggcg tggcgttagt
     1621 ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc caccgccaac
     1681 cagccaggct tcgtatcccg ctttattgag cctgtacatt accttcaggg cattttcact
     1741 gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct gtggacgggc
     1801 gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac
     1861 tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg
     1921 ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc cgactcaggc
     1981 acggcggtaa gccgccagtt tttgacggct gactgaagga tttgctcgac gctgaaatcc
     2041 tgccagtgtg cttctgcctg ctgacccaga aattgaagtg ccgcgattag taccgggcgc
     2101 ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc gccttgtgtg
     2161 ttaagcgcca gcggtaaatg aatgtaatct ggcactttcc agccaaaaag ctggtacagc
     2221 gagatttgcc gcactgtcgg ttcgatcaaa tctgccccgc ggacaatttc gctcacgccc
     2281 tggaagtgat catccaccac caccgccagg ttataggcaa acaaaccatc acggcgatga
     2341 ataataaaat cttcccgcgc cagtttttcg ttggcgtgaa taatgccgcg cagctgatca
     2401 gtaaatagcg tgaccggatg ctgctggcgg atacgtactg cggcgttgtc tggtccatga
     2461 tgcaattccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg cgcgcgcgtg
     2521 caggtgcagt aataacttag tccttgctca tgtaaccagg cgagtgcttc gcgataggca
     2581 tcgtgacgtt gcgattgcca gagaacgtcg ccgtcccagt gcagaccgta atgttccagc
     2641 tggcgcagga tagtctctgc ggcaccggga acttcacgag gcgggtcgat atcttcaatg
     2701 cgtaccagcc agcgaccttg ccgggcgcga gcctgtaaat agctgccaag cgcggcaatc
     2761 agagagccaa aatgaagctc gccggaagga gagggggcaa agcggccaat atagtgtgtg
     2821 tctgtcatct cgttgaacaa aaaataaggc gggagcattt cccgcctgtg gtaaacgtga
     2881 tggaacggct gtatttagcc agccatttgt ttttcgcgaa tttcagccag cgttttgcag
     2941 tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat ttcaacaccg
     3001 caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt ctcgatcttt
     3061 ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc ttcttcctgg
     3121 gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg tgtaacggtg
     3181 cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg gaagtgcgcc
     3241 agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc caccccagcg
     3301 atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat gttgcttctc
     3361 cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta taaatagcag
     3421 atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg aggaaaagcg
     3481 tatttgcgca cgcgaccaga atgtaaatta accagttaca tactttacta caatgtaacc
     3541 ggcagtgatt ttttaagagc catgccttca gcagaaagtt ccgctttgta agccagaatt
     3601 tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc gatgtggcgc
     3661 gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac cgcacgctgg
     3721 ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc agtgaccgca
     3781 tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc aatatagcag
     3841 tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcggc gccgtatttt
     3901 acttcgcttt tcagcgagct atagcctgac agctctgaaa ttgattcatt aaggatagcc
     3961 tctttcgtca acctgttagc ccaaagcgtg ttgacgcaaa taaatgcgcc gctctggctt
     4021 tgagtcaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt cgaataccag
     4081 acggtatcgc caggcgttgc acaactggtc atcgcacccg tattcgggca gtgtagcgtt
     4141 aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc
     4201 gtcgcgcgct gtagaggggg agaaaattcc attacgacaa tccttgttat tgcgttagcg
     4261 cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag gcgtaaaggg
     4321 tgaactccgt caccgtatac gaccagttaa aacctggcgg cgggattgtc accgcctcgc
     4381 tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga aaacaaccgc
     4441 tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt ggtggctgac
     4501 gcatccctaa ccacaccaca cgcgaacgca gccattgacc ggcgtcatca agcgtgagtg
     4561 tgaaacccgg ttgacgaatc cgcccggcta aaagagaaag cgccttctct ttctcggcgc
     4621 tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc ggacgtcccg
     4681 cgtcaggtgg gaagtgtgtg gcgcgccagt ggataatctg ttcgcggatt tctgcaggta
     4741 agtcgatagc aaagaacagc cgttgcggtt cagacatgtg aggcactcgg ttatgaatta
     4801 ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt
     4861 tgcccgttgc tgccgtctta cctgagttac ttgctgctct cgattgtgca tcgcaggtgt
     4921 tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa ctgctggcgc
     4981 atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg gcgcgtaacg
     5041 ttgcgcaacg actggcggag ctgcttaacg aaaagccagg cgataccgtt ggctaccgga
     5101 tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc gaaggcgtgc
     5161 tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg atccttgatg
     5221 aatttcatga gcgcagcttg caggcggatt tggcgttggc gctgttactc gatgtgcaac
     5281 aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac aacgaccgct
     5341 tgcagcaaat gctgccagaa gcgcccgtcg tcatctcaga agggcgctcg tttccggttg
     5401 aacgccgtta tttgccgctg cccgctcatc agcgttttga cgaagccgtc gcggttgcca
     5461 ccgccgaaat gctgcgtcag gaaagtggat cattactgtt atttttacct ggcgtcggag
     5521 aaattcagcg tgtgcaggaa caactggctt cacgcatcgg cagtgatgtg ttgctctgcc
     5581 cgctgtatgg cgcgttgtcg ctgaacgatc agcgaaaagc gatcctcccg gcaccgcaag
     5641 ggatgcgtaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc atcgaaggta
     5701 ttcgtctggt ggtggattgt gcccaggagc gtgtggcgca ttttgatccg cgtacagggc
     5761 ttacgcggct ggttactcaa cgcgttagtc aggcgtcgat gacgcaacgt gccgggcgtg
     5821 ccgggcgtct ggagccgggt atctgcctgc atttaatcgc caaagaacaa gcagaacgcg
     5881 ctgccgcgca aagtgaaccg gagatcttac aaagcgatct ttccggtttg ctgatggaat
     5941 tactgcaatg gggatgcagc gatacggcgc agatgagctg gctggatcaa ccgccaacgg
     6001 tgaatctaca ggccgcgaaa cggctgttac aaatgctggg ggcgctggag ggtgaacggc
     6061 ttagcgcaca agggcaaaaa atggcagtgc tgggtagcga tccgcgttta gcggcaatgc
     6121 tggttagcgc gaagaacgac gacgaagctg ctaccgcggc aaaaattgcc gccattctcg
     6181 aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc aatcaacccg
     6241 cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag
     6301 acagttcgct tatcgcgccg ctacttgccg gggcgtatgc cgatcgcatt gcttgtcgcc
     6361 gtgggcaaga tggacgctat caactggcaa acggcatggg ggcgatgctc gatgccgacg
     6421 atgcgctaag ccgctacgaa tggttgatcg caccgttatt attgcagggc agcgcctcgc
     6481 cggatgcgcg gattttactg gcgctaccgg tcgatatcga tgaattagta caacgctgtc
     6541 cgcagctggt acagcagtcg gacaccgtgg aatgggatga cgcgcaaggc acgctgaaag
     6601 cctggcgtcg gctgcaaatc ggtcagttga cggtgaaagt gcagccactg gcgaaaccct
     6661 cggaagacga gttgcatcag gcgatgctta acggcatccg tgataaaggt ttaagcgtgc
     6721 tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttattcgcc gcaaagtggt
     6781 tgccggaata tgactggcca gcggttgatg atgagagttt gttggcgacg ctggaaacgt
     6841 ggctactgcc gcatatgagc ggcgtgcatt cactacgcgg cctgaaatca ctcgacattt
     6901 atcaggcgct acgcggatta cttgattggg gaatgcagca acgtctggat agtgaattgc
     6961 ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat gaagataaac
     7021 cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccaccaat ccgacgatcg
     7081 cccaggggcg cgtgccgctg gtgctggagt tgctttcccc tgcccaaagg ccattacaaa
     7141 tcacacgaga tttgagcgcc ttctggaaag gagcgtaccg tgaggtgcaa aaagagatga
     7201 aagggcgtta tcccaaacat gtctggccgg acgacccggc aaatactgca ccgacgcgac
     7261 ggacgaaaaa gtattcgtga agaccaaagc aaatttgaga gatatcttct tctgtcttgc
     7321 aacagaagaa cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg
     7381 ggaatgaccg cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg
     7441 taagccgtcg tcgttacgaa gatgacgatt acgacgatta tgatgactat gaggatgaag
     7501 aaccgatgcc gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt ggcaaacgcg
     7561 gctggctatg gctgctgcta aaactggcta tcgtttttgc cgtgctgatc gccatttacg
     7621 gcgtttatct cgatcaaaaa attcgcagcc gtattgatgg caaggtctgg caattgcccg
     7681 cggcagttta tggccgaatg gtcaaccttg agccagacat gaccatcagc aagaacgaaa
     7741 tggtgaagct gctggaggcg acccagtatc gtcaggtgtc gaaaatgacc cgtcctggcg
     7801 aatttaccgt gcaggccaac agcattgaga tgatccgccg tccgtttgat ttcccggaca
     7861 gtaaagaagg gcaggtgcgt gcgcgtctga cctttgatgg cgatcatctg gcgacgatcg
     7921 tcaatatgga gaacaaccgt cagtttggtt tcttccgtct tgatccgcgc ctgatcacca
     7981 tgatctcctc gccaaacggt gagcagcgtt tgtttgtgcc gcgcagtggt ttcccggatt
     8041 tgctggtgga tactttgctg gcgacagaag accgccattt ctacgagcat gatgggatca
     8101 gtctctactc catcggacgt gcggtgctgg caaacctgac cgccggacgc acggtgcagg
     8161 gtgcgagtac gctgacgcaa cagctggtga aaaacctgtt cctctccagc gagcgctctt
     8221 actggcgtaa agcgaacgaa gcttacatgg cgctgatcat ggacgcgcgt tacagcaaag
     8281 accgtattct tgagctgtat atgaacgagg tgtatctcgg tcagagcggc gacaacgaaa
     8341 tccgcggctt cccgctggcg agcttgtatt actttggtcg cccagtagaa gaattgagcc
     8401 tcgaccagca ggcgctgttg gtgggtatgg tgaaaggggc gtccatctac aatccgtggc
     8461 gtaacccgaa actggcgttg gagcgacgta atctggtact gcgtctgctg caacagcaac
     8521 agattattga tcaagaactc tatgacatgt tgagtgcacg tccgctgggg gttcagccgc
     8581 gcggtggggt gatctctcca cagccagcct ttatgcaact ggtgcgtcag gagctgcagg
     8641 caaaactggg cgataaggta aaagatctct ccggcgtgaa gatcttcact acctttgact
     8701 cggtggccca ggacgcggca gaaaaagccg ccgtggaagg cattccggca ctgaagaaac
     8761 agcgtaagtt gagcgatctt gaaaccgcga ttgtggtcgt cgaccgcttt agtggtgaag
     8821 ttcgtgcgat ggttggaggt tctgagccgc agtttgcggg ctacaaccgt gcgatgcagg
     8881 cgcgtcgttc gattggttcc cttgcaaaac cagcgactta tctgacggcc ttaagccagc
     8941 cgaaaatcta tcgcctgaac acgtggattg cggatgcgcc aatcgcgcta cgtcagccga
     9001 atggccaggt ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc ggcagagtga
     9061 tgctggtaga tgcgttgacc cgttcgatga acgtgccgac ggtgaatctg ggcatggcgt
     9121 tggggctgcc tgcggttacg gagacctgga ttaaactggg tgtgccgaaa gatcaactga
     9181 atccagttcc agcaatgttg ttgggggcgt tgaacttaac gccaatcgaa gtggcgcagg
     9241 cgttccagac catcgccagc ggtggtaacc gtgctccgct ttctgcactg cgttcggtaa
     9301 tcgcggaaga tggcaaggtg ctgtatcaga gcttcccgca ggcggaacgc gctgttccgg
     9361 cgcaggcggc gtatctgaca ctatggacca tgcagcaggt ggtacaacgt ggtacggggc
     9421 gtcagcttgg ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg actaccaaca
     9481 ataacgtaga tacctggttt gcgggcattg acggcagtac ggtgaccatc acctgggtag
     9541 gccgtgataa caaccaaccg accaaattgt atggagccag cggggcaatg tcgatttatc
     9601 agcgttatct ggctaaccag acgccaacac cgctgaatct tgttccgcca gaagatattg
     9661 cagatatggg cgtggactat gacggcaact ttgtttgcag cggtggcatg cgtgtcttgc
     9721 cggtctggac cagcgatccg caatcgctgt gccagcagag tgagatgcag cagcagccgt
     9781 caggcaatcc gtttgatcag tcttctcagc cgcagcaaca gccgcaacag caacctgctc
     9841 agcaagagca gaaagacagc gacggtgtag ccggttggat caaggatatg tttggtagta
     9901 attaacgtct aagcgtgaca taccggatgg cgagtagcca tccggtaaaa taacatccca
     9961 cccaaagtat taaccctttc ttttcatctg gttgtttatt aacccttcag gaaagctcag
    10021 attgcgtacc gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt tataataatc
    10081 attctcgttt acgttatcat tcactttata tcagagatat accaatggcg cgttccaaaa
    10141 ctgctcagcc aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca gcggttagcg
    10201 gcatgtctgt ttatgcacag gcagcggttg aaccgaaaga agacactatc accgttaccg
    10261 ctgcacctgc gccgtaagaa agcgcatggg gaccggctgc aactattgcg gcgaagcact
    10321 ctgctactgc gactaaaacg gatacaccaa ttgaaaaaac gccgcagtct atttcggttg
    10381 tgacaaatga agagatgcag atgcatcaat ttcagtctgt aaaagaagca ttaggttata
    10441 cacctggcgt tacggttagc agtcgtggtg cttctaatac atatgatttt gttattattc
    10501 gtggtttctc atctgttggt ctgaatcaaa ataattacct tgatggacta aaacttcagg
    10561 gcaactttta taacgatgct gtgattgatc cttacatgct cgagagggtt gaactgatgc
    10621 gcgggccgac gtctgttctc tatgggaaaa gtaatcctgg tgggattatt tctatggtga
    10681 gtaagcgtcc gacaactgaa cccctgaaag aaattcagtt taaaatgggg acggataatc
    10741 tgtttcagac cggattcgat ttcagtgatg cgctggatga taacggtgag ttctcctacc
    10801 gtttgactgg cctcgcacgc tcaacaaacg aacagcagaa aaactctgaa tctcagcgtt
    10861 ataccattgc tccatcattc tcatggcgtc cagacgacaa aaccaatttt actttcctgt
    10921 cctattttca gaacgaacca gaaacgggtt attacggttg gttgccgaaa gaggggaccg
    10981 ttgagccatt gcctaatggt aagcgtctac cgactgactt caatgaaggt gcatcgaata
    11041 atacatactc ccgtaaccag aaaatggtgg gatatagttt tgaacatggt tttaatgaca
    11101 ccttcaccgt gcgtcagaat ctgcgtttca gtgaaatgaa aacctcacag aaaagtgttt
    11161 atggcacagg gattgccagc gatggtcata ccctaaaccg cgggacagtg gtggataatg
    11221 agcgtctgca aaactttagc gttgataccc aacttgaaag taaatttgct acaggtgaag
    11281 tcgagcatac tttgctgaca ggggtagact tcatgcgtat gcgcaatgat atcaatgcca
    11341 gctttggatc tgcaccatcc atcgatcttt ataacaaata tcatcctgaa tactttgcat
    11401 ttggtaacgc agagccatac caaatgaatg aaagcaaaca aacaggtatt tatgttcagg
    11461 atcaggcgga atggaataaa tgggtattca ctctgggggg acgctacgat tggtctaagc
    11521 aagcgaccac tgttcgtgaa aactcttata cgccgactga aggttatatt gagcgtaatg
    11581 atcatcagtt cacctggcgc ggtggtgtaa actacttatt cgataatggt atttcacctt
    11641 actttagtta tagccagtcc tttgaaccga gtgctttcga tctgtggagc aacccgcgcg
    11701 tttcctataa gccatcgaaa ggtgaacagt atgaagctgg cgtaaaatat gttccgaatg
    11761 atatgccggt cgttgttacg ggcgcagtct atcaattgac gaaaacaaat aacctgacag
    11821 cagacccaac aaacccgtta gcgcaagtcc cagcaggtga gattcgcgct cgtggtgtgg
    11881 aacttgaagc aaaagcggcg ttaaatgcca atattaactt gacggcttct tatacctaca
    11941 cagatgcgga atacaccaaa gacaccaatc tcaaaggtaa aactccagaa caagtaccgg
    12001 agcatatggc atctctctgg ggggattata ccttcaatga agggccgctt tctggtttaa
    12061 cattgggaac aggtggtcgt tttattggtt ccagctatgg tgatccggcg aacactttta
    12121 aagtgggtag cgcagctgta atggatgctg ttgtaaaata tgatctggca cgctttggta
    12181 tggcgggatc cagccttgct gtgaacgtca acaatttgct cgatcgtgag tatgttgcca
    12241 gttgcttcca gacctatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa
    12301 ccttccgttt ctaatttttt ctttggggca cggatttccg tgcccgcttc acaagttggc
    12361 tgttatgcag gaatacacga atcattccga taccactttt gcactgcgta atatctcctt
    12421 tcgtgtgcca gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt
    12481 gactggtttg attggtcaca acggttctgg taaatccact ctgctcaaaa tgcttggccg
    12541 tcatcagcca ccgtcggaag gcgaaattct tcttgatgcc cagccgctgg aaagctggag
    12601 cagcaaagcg ttcgcccgca aagtggctta tttgccgcag cagcttcctc cggcagaagg
    12661 gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg tggcatggcg cactggggcg
    12721 ctttggtgcg gcagatcgcg aaaaggtcga agaggcgatt tcgctggttg gcttaaaacc
    12781 gctggcgcat cggctggtcg atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc
    12841 tatgctggtg gcgcaggata gccgttgtct gttgctcgac gaaccgacct cggcgctgga
    12901 tatcgcccac caggttgatg tgctggcgct ggtgcatcgt ttaagtcagg aacgtggtct
    12961 gacggtcatt gccgtgctgc acgatattaa tatggctgcg cgctactgtg attatctggt
    13021 cgccctgcgc ggtggtgaaa tgattgctca gggaacgcct gcggaaatta tgcgcggcga
    13081 aaccctcgaa atgatttatg gcatcccgat gggtattctg ccgcatccgg cgggtgctgc
    13141 acctgtgagt tttgtttatt gatgagcggc ttacctctta tttcgcgccg ccgactgtta
    13201 acggcgatgg cgctttctcc gttgttatgg cagatgaata ccgcccacgc ggcgattatt
    13261 gatcccaatc gtattgtggc gctggagtgg ttgccggtgg aattactgct ggcgctcggc
    13321 atcgtgcctt acggcgtggc ggacaccatc aactatcgcc tgtgggtcag cgaaccgcca
    13381 ttgccggact cagtgattga cgttggtttg cgtacagaac ccaaccttga actgctgacc
    13441 gaaatgaaac cgtcgtttat gatctggtcg gcaggatatg gcccttcacc agaaatgctg
    13501 gcacgtattg cgccggggcg cggatttaac ttcagcgacg gcaaacagcc gctggcgatg
    13561 gcgcgtaaat cactgacgga aatggcagat ttacttaacc tgcaaagcgc agcagaaacg
    13621 catttagcgc aatatgaaga ctttatccgc agcatgaaac cccgctttgt gaagcgtggt
    13681 gcacgcccgt tattgctgac gacgcttatc gatccgcgcc atatgttggt cttcggtcca
    13741 aacagcttgt tccaggaaat tcttgatgag tacggcatcc caaatgcctg gcaaggggaa
    13801 accaacttct ggggcagtac cgccgtcagt atcgatcgtc tggcggcgta taaagacgtt
    13861 gatgtgctct gttttgatca cgacaacagc aaagacatgg atgcgctaat ggcaaccccg
    13921 ctgtggcagg ccatgccgtt tgtccgtgca ggacgttttc agcgcgttcc ggcggtctgg
    13981 ttctatggcg cgacgctttc ggcaatgcac tttgtgcgta ttctggataa cgccatcgga
    14041 ggtaaagcgt gagtaaacga attgcgcttt ttccggcgtt attgctggcg ctgctggtga
    14101 ttgtcgctac ggcgctcacc tggatgaact tctcgcaggc gttgccgcgc agccagtggg
    14161 cgcaggccgc ctggtcgccg gatattgacg tcatcgagca gatgattttt cactacagct
    14221 tgttgccgcg tctggcgatt tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc
    14281 tgtttcagca agtgctgcgt aacccgctgg cggaaccgac gacgttgggc gttgcaacag
    14341 gcgcgcaact ggggattacc gtcactacgc tctgggcgat cccaggtgct atggcgagcc
    14401 agtttgctgc gctggcaggg gcttgcgttg ttggcttaat tgtctttggc gtcgcgtggg
    14461 ggaaacggct gtcgccggta acgctgatcc tcgcggggct ggtagtgagc ctttattgtg
    14521 gggcaatcaa tcagttactg gttatcttcc atcacgacca gctacaaagc atgttcctgt
    14581 ggagcaccgg aacgctgacg caaaccgact ggggcggcgt tgagcgttta tggccgcagc
    14641 tgctgggcgg cgtgatgctg acgttattgc tacttcgccc gttaaccctg atggggcttg
    14701 atgatggcgt ggcgcgcaat ctcggtctgg ccttgtcgct ggcgcgtctg gcggcgctgt
    14761 cgctggcgat agtcatcagt gcgctgctgg tgaatgcagt ggggattatc ggctttatcg
    14821 ggttgttcgc gccgctgctg gcaaaaatgc tgggggcgcg gcgtctgctg ccacggttaa
    14881 tgctggcgtc gctgattggt gcgttaattc tgtggctttc tgatcaaatc atcctctggc
    14941 tgactcgcgt gtggatggaa gtttccaccg gttcggtcac tgcgttgatc ggtgcgccgc
    15001 tgctactgtg gctgctgccg cgtttacgca gcattagcgc gccggatatg aaggtcaacg
    15061 atcgtgtcgc ggctgaacgc caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgc
    15121 tgttgatggc tgtggtagtg gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg
    15181 cgagcggggc gttgctcgag gatttaatgc cctggcgctg gccgcgaatt atggcggcgc
    15241 tgtttgcggg cgtcatgctg gcggtggcgg gctgtattat tcagcggctg accggaaacc
    15301 cgatggcaag cccggaagtg ctggggatta gctccggcgc ggcgtttggc gtggtgttga
    15361 tgctgtttct ggtgccgggt aatgcctttg gctggctgtt acctgcaggc agtctcggcg
    15421 cggcggtgac gctgttgatc attatgatcg ccgccggacg cggtggattt tccccacacc
    15481 gcatgttact ggcggggatg gcgttaagta ccgcgttcac catgcttttg atgatgttgc
    15541 aggcaagtgg tgacccgcga atggcgcaag tgctgacctg gatctccggt tcgacctaca
    15601 acgcgaccga tgcgcaggtc tggcgcacgg gaattgtgat ggtaattttg ctggcgatta
    15661 acccgctgtg ccgccgctgg ctgaccattt taccgctggg cggtgatacc gcacgagccg
    15721 taggaatggc gctgacgccg acgcgaattg cgctgctgct attagcggct tgcctgacgg
    15781 cgaccgcgac gatgactatt ggaccgttga gttttgttgg tttaatggca ccgcatattg
    15841 cgcggatgat gggttttcga cggacgatgc cacacatcgt aatttcggcg ctggtgggtg
    15901 gtttactgct ggtgttcgct gactggtgtg ggcggatggt attgtttcca ttccagatcc
    15961 cggcggggct gctgtcaacc tttatcggcg cgccatattt tatctatttg ttgagaaagc
    16021 agagccgtta atagaatgcc tcgcccggtg ggcgaggcaa aatcatcaca acttcgcaaa
    16081 cacccgacgt gcagcatcga tggtgttatt gatatcttcc atgctgtgcg ccacggacat
    16141 aaagcccgct tcaaacgctg acggtgccag ataaacacct tcgtccagca tcatatggaa
    16201 gaaacgctta aagcgttcca cgtcacaggc catcacatcc tgatagcacg tcacggactc
    16261 ggcgtcggta aagaaaatac cgaacatgcc gccaacgtgg tttaccacca gcggaattcc
    16321 ggcttcttct gccgcttcca gcagaccttc tgccagacgt gttgtcagct catccagcgt
    16381 ctcgtgaacg cccggctgtg cgacttcatt cagacaggcg aaaccagctg ccatcgcaat
    16441 cgggttaccg gaaagcgtac ccgcctgata gaccggacca gtcggggcca gcgcatccat
    16501 tacatcacga cgaccaccga atgcgcctac cggcattcca ccgccgatga ttttgccgag
    16561 gcaggttaaa tctggcacta cgccgtaata atcctgtgcg ccagctagcg ctacgcggaa
    16621 accggtcatc acttcatcga tgatcagcaa cgcgccaaat tcgtcacaca gcgcacgcag
    16681 acctggcagg aactctggca gcggtggaac gcagttcata ttgcctgcca ccggctcgac
    16741 gataatacag gcaatctcat gcgggtactg ttcgaacgcg gcgcgtacag aggccagatc
    16801 gttataagta caggttaagg tatgtttggc gaaatctgcc ggaacacccg gcgagtttgg
    16861 ctggcctaac gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag cgtgaccgtg
    16921 gtaacaacct tcaaatttaa tgattttgtc gcgaccggta aaaccacggg ccaggcggat
    16981 tgcgctcatg gtcgcttcag tgccggagtt caccatgcgc accatatcca tggtcgggac
    17041 cagttcggtc accagttgcg ccattttcac ttccatttcg gtcggtgcac caaagcttaa
    17101 accacgctcg gcggcttcaa tcacggcatt gcggatcgcc ggatggttat ggcccagcac
    17161 catcggcccc caggaaccga cataatcgat ataggctttg ccatcaacat cgtacagata
    17221 agcgccgtcc gctttttcga taaacagtgg agtgccgccc acgccagtaa aggcgcgaac
    17281 aggggagttc acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat tttcagactt
    17341 actcatggag ggttcctgat tcgtagaaaa agtgaatggc tgctattcta tgttattcat
    17401 aacaagttaa atactcgtca aacatcaggc tgcttgtact ggtgcaatcc tgatttctta
    17461 gagtataaaa gttttgtgca tttgaaacat tacgctttgc aaaggatttt catggaacgt
    17521 gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag tgatcattgg atgaaaactg
    17581 atactccctc tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa ctgattcgcc
    17641 aacttcttga gcgcgataaa accccgttag ccattttgtt tatggcggca gtcgtcggca
    17701 cgcttgtcgg gctggctgcg gttgcttttg acaaaggtgt cgcctggttg cagaaccaac
    17761 gtatgggggc actggtacat actgctgata attatccgct actgttaacc gtcgcttttc
    17821 tctgttcggc ggtgctggcg atgttcggct actttttggt gcgcaaatac gcgccggaag
    17881 caggtggttc ggggatcccg gaaattgaag gggcgctgga agatcaacgt cccgttcgct
    17941 ggtggcgtgt gttgccggtg aaattctttg gcgggctggg aacactcggc ggaggcatgg
    18001 tgctgggccg cgaagggcca accgtacaga tcggcggcaa tatcggtcgc atggtgcttg
    18061 atgttttccg cctgaaaggt gatgaagcgc gccatacgct gctggcaacc ggtgctgcgg
    18121 cggggctggc tgcggccttt aacgccccgc tggcgggtat tttgtttatt atcgaagaga
    18181 tgcgtccgca gtttcgctat acgttaattt cgattaaagc ggtatttatt ggcgtcatta
    18241 tgtcgaccat tatgtaccgg atttttaatc atgaagttgc gttgattgac gtcggtaaac
    18301 tttctgacgc gccgcttaat acgctgtggc tttatctgat cctcggtatt atttttggca
    18361 ttttcggccc tatttttaat aaatgggtgc tggggatgca ggatttgctg caccgtgtgc
    18421 acggcggcaa tattaccaaa tgggtactga tgggcggtgc aattggcggt ctgtgtggat
    18481 tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt taacctgatt cctatcgcta
    18541 ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt cgtcgcgcgg gtcattacca
    18601 ccttactctg cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg ctggcgctgg
    18661 gtactgtgct gggaaccgct ttcggaatgg ttgccgttga gctgtttccg caatatcacc
    18721 ttgaggcggg gacatttgct attgccggaa tgggggcatt actggcggca tctattcgcg
    18781 cgccgttaac ggggatcatt ctggttctgg aaatgaccga taactaccag ctcattttgc
    18841 caatgattat taccggtctt ggcgcaacac tattagcgca atttaccggc gggaaaccgc
    18901 tatactcggc gattcttgcg cgcacgctgg cgaaacagga agctgagcaa ctggcgcgaa
    18961 gcaaggccgc atcagccagc gagaatactt gaacgaaata ccagggtatt agataatggc
    19021 gattattatt gggttagaat ttgcccaatt gccgatgtcg tttggagcaa aatatgagtg
    19081 atgacgtagc actgccgctg gagtttaccg acgcagcagc caacaaagtt aaaagcctga
    19141 tcgctgacga agataacccg aatctgaaat tacgcgtgta tattaccggt ggcggttgca
    19201 gcggcttcca gtatggtttc acctttgatg atcaggtgaa cgaaggcgat atgaccatcg
    19261 aaaaacaggg cgttggcctg gtggttgatc cgatgagcct gcaatatctg gtcggcggtt
    19321 ccgttgacta taccgaaggt ctggaaggtt ctcgtttcat cgtgaccaac ccgaacgcga
    19381 aaagcacctg cggttgcggt tcttccttta gcatctaatc tgttgttcat gtgccggata
    19441 aactatccgg cacatcttct ctcttcaacg cccattctca tccagcgcaa acgtcggtag
    19501 cttaagatgc caacgaatag ccgccagccg aatcaatagc gtcacgacca tgcccatcat
    19561 actggctgtt tccagtggta cggaaaatgt gtaataagcc gtagcgtgga caataccgcc
    19621 gataatacag gctgttgcgt agatttctgt acgtaaaatc atggggatct cgcgggcaag
    19681 tacatcacga atgatcccgc cgccaacgcc agtaatgacg cccatacaaa ccgcgattaa
    19741 cggaccggct tccgcattaa aggctttatt cacgccaatg ccgacaaaca ctgccagacc
    19801 aacggcgtcc agcaccggca acatccattt tggtaagcgt cttggctggc gcaccagcac
    19861 gatggtcagc atgctggtga ccattgcaac gaccagatcg gtgggatctt tcacccaaaa
    19921 taccgggccg tgatccagcg ccatgtcgcg aattgttccg ccgcctactg cggtaaccac
    19981 gcccaatacc agaacaccaa aagggtccat acgcaatttt ccggctaaca aaacgccaga
    20041 gatagcaaat accgctgtgc cgactatatc cagccaatag acgagcattg ttcaatcccc
    20101 actgagcacc tgaaaaggtc aggcattaat ctacctgtga aagcgcatta cagagctgtt
    20161 gtgcagcgag gataatacgt gggcttgcac gttcaaacca gtcactcgtg agaggaataa
    20221 cgggaatttt gagctgttca ccccagtatt gtttgatttt aggaatttgg tccggtccgc
    20281 ctgtaatgac aatcgcctgt ggcgcgcgtg ctaacacctg ttcgcggcta acttgcggcc
    20341 agggaacccg gctgtcttta aagatgtttt ctccgccaca aacttcgagt acctggtttt
    20401 gaatcgactc ttttccacta gtaaatggcg gattaatgcc gaattgcaga aaaacacgtt
    20461 ttttaggttt atcagcatat tgcgctttca attgcgcgta ctggtccagc agggattgcg
    20521 cggcttgttc ggccttatcc ggttgcggac tccaggggac cagttgacgt aacgcattgg
    20581 caatttgttc aatgcttgtc gcatcgaccc acatcacttt tattcccaga gaagccagct
    20641 ggtcaacctg ccgctcggca ttacctccac gccaggcaat caccagatcg ggtttcagcg
    20701 ccacaatgcg ttccagattc atcccctgcc aggtggaaac ctgctcaatt ttttgcgctt
    20761 gtggaggata gtcggaatag ctgctgaccc caaccggcgt gatcccggcg gcaaaggcaa
    20821 gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc cacagcggcg
    20881 cgagaaaaga cagggcgacc agcgccctga acagtgactt agccatgtgc cagtttctgc
    20941 accagtgact caaccatcag gctggactgt ttagcggcaa cagccaggaa ctcatcgaag
    21001 ctaagatgag actgttgatc ggccacgtcg gaaatggcgc gtacgacaac aaacgggacg
    21061 ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca tctctacagc aatggcctgt
    21121 gggaagttgt ggcggatttt cgccagacca acagaaccat tgatgaaagc gtcgccgctg
    21181 acaatcaggc cacgtacagc gtttagattc agttcggcaa tgcaggcctc agcggcagcg
    21241 atcagtttat cgtcagcttt aaagcctgcc gggcagcctg gtaactggcc gtattcataa
    21301 ccaaatgccg tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac gatatcgccc
    21361 actttcagtg ttggtgccag gccaccggca gaaccggtgt taataatcac atctggcttg
    21421 cagtgttcca acagcaaagt ggcacccagc gccgcagcga ctttaccgat gcccgatttc
    21481 agaagcgcaa cctcggttcc attcagttgg ccggtataga tttcgcaacc gccgagactg
    21541 atagtttgac ggttttcgat tttgtcacgc agtagcgtaa cttcttcttc cattgcacca
    21601 atgatgccga ttttcataga tttactcgcg ataagcccga tttgaaggca tagtttacca
    21661 tgcgcttacg gggaagcgta tttctcacgc gggagaggac atggcacaga ttgatttccg
    21721 aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa ccgaacatga
    21781 gatcctacgg atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg caattcgtcg
    21841 tctgcaacaa aaaacccagg tttttccact ggagcgcaat gccgccgtgc gcacgcgtct
    21901 tacccactcg atggaagtcc agcaggtggg gcgctacatc gccaaagaaa ttttaagccg
    21961 tctgaaagag cttaaattac tggaagcata cggcctggat gaactgaccg gcccctttga
    22021 aagcattgtt gagatgtcat gccttatgca cgatatcggc aatccgccgt ttggtcattt
    22081 tggcgaagcg gcgataaatg actggtttcg ccagcgtttg cacccggaag atgccgaaag
    22141 ccagcctctg actgacgatc gctgcagcgt ggcagcacta cgtttacggg acggggaaga
    22201 accgcttaac gagctgcggc gcaaaatacg tcaggactta tgtcattttg aggggaatgc
    22261 acaaggcatt cgtctggtgc atacattgat gcggatgaat ctcacctggg cacaggttgg
    22321 cggtatttta aaatatactc gtccggcgtg gtggcgtggt gaaacgcctg agacacatca
    22381 ctatttaatg aaaaagccgg gttattatct ttctgaggaa gcctatattg cccggttgcg
    22441 taaagaactt aatttggcgc tttacagtcg ttttccatta acgtggatta tggaagctgc
    22501 cgacgacatc tcctattgtg tggcagacct tgaagatgcg gtagagaaaa gaatatttac
    22561 cgttgagcag ctttatcatc atttgcacga agcgtggggc cagcatgaga aaggttcgct
    22621 ctattcgctg gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt taagccgcag
    22681 tacggaagat cagtttttta tgtatttacg ggtaaacacc ctaaataaac tggtacccta
    22741 cgcggcacaa cgatttattg ataatctgcc tgcgattttc gccggaacgt ttaatcatgc
    22801 attattggaa gatgccagcg aatgcagtga tcttctaaag ctatataaaa atgtcgctgt
    22861 aaaacatgta tttagccatc cagatgtcga gcagcttgaa ttgcagggct atcgggtcat
    22921 tagcggatta ttagagattt atcgcccttt attaagcctg tcgttatcag actttactga
    22981 actggtagaa aaagaacggg tgaaacgttt ccctattgaa tcgcgcttat tcaacaaact
    23041 ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt aaattaccgt cagattctcc
    23101 tgagtttccg ctatgggaat attattaccg ttgccgcctg ctgcaggatt atatcagcgg
    23161 tatgaccgac ctctatgcgt gggatgaata ccgacgtctg atggccgtag aacaataacc
    23221 aggcttttgt aaagacgaac aataaatttt taccttttgc agaaacttta gttcggaact
    23281 tcgcgctata aaacgaatct gaagaacaca gcaattttgc gttatctgtt aatcgagact
    23341 gaaatacatg aaaaaaacca cattagcact gagtgcactg gctctgagtt taggtttggc
    23401 gttatctccg ctctctgcaa cggcggctga gacttcttca gcaacgacag cccagcagat
    23461 gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga
    23521 aggtagcaca accgttaata cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga
    23581 tgattctccg ttctgccagg aaggttctcc gttccagagc tctccgttct gccagggggg
    23641 ccagggcggt aatggtggcg gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat
    23701 cattgatgcc gataaaggct atgtcgtcac caacaaccac gttgttgata acgcgacggt
    23761 gattaaagtc cagctgagcg atggccgtaa gttcgacgcg aagatggttg gcaaagatcc
    23821 gcgctctgat atcgcgctga tccagatcca gaacccgaaa aatctgaccg caattaagat
    23881 ggcggattct gatgcactgc gcgtgggtga ttacaccgta gcaattggta acccgtttgg
    23941 tctgggcgag acggtaactt ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc
    24001 tgaaaactac gaaaacttca tccagaccga tgcagcgatc aaccgtggta actccggtgg
    24061 tgcgttggtt aacctgaacg gcgaactgat cggtatcaac accgcgatcc tcgcaccgga
    24121 cggcggcaac atcggtatcg gttttgctat cccgagtaac atggtgaaaa acctgacctc
    24181 gcagatggtg gaatacggcc aggtgaaacg cggtgagctg ggtattatgg ggactgagct
    24241 gaactccgaa ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca
    24301 ggttctgcct aactcctccg ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc
    24361 actgaacggt aagccaatca gcagctttgc cgccctgcgt gctcaggtgg gtactatgcc
    24421 ggtgggcagc aaactgaccc tgggcttact gcgcgacggt aagcaggtta acgtgaacct
    24481 ggaactgcag cagagcagcc agaatcaggt tgattccagc tccatcttca acggcattga
    24541 aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca acgtgaaaac
    24601 gggcactccg gctgcgcaga tcggcctgaa aaaaggtgat gtgattattg gcgcgaacca
    24661 gcaggcagtg aaaaacatcg ctgaactgcg taaagttctc gacagcaaac cgtctgtgct
    24721 ggcactgaac attcagcgcg gcgacagcac catctacctg ttaatgcagt aatctccctc
    24781 aaccccttcc tgaatacggg aaggggttct ccttacaatc tgtgaacttc gccacaactc
    24841 catacatctt catcatcctt tgggcatttg cacaatgccg tacgttacgt acttccttat
    24901 gctaagccgt gcataacgga ggacttatgg ctggctggca tcttgatacc aaaatggcgc
    24961 aggatatcgt agcacgtacc atgcgcatca tcgataccaa tatcaacgta atggatgccc
    25021 gcgggcgaat tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac gaaggagcat
    25081 tgctggtact ttcacaggga cgagtcgtcg atatcgatga cgcggtggca cgtcatctgc
    25141 acggtgtgcg gcaggggatt aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa
    25201 ttggcctgac aggtgaacca gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg
    25261 ctgaaatgat gctggaacag tcgcggttga tgcacctgtt ggcgcaggat agccgtttgc
    25321 gggaagaact ggtgatgaac ctcattcagg ctgaggagaa tactcccgca cttactgaat
    25381 gggcgcaacg gctggggatc gatctcaatc aaccgcgagt ggtggctatt gttgaggtcg
    25441 acagcggtca gcttggcgtg gacagcgcaa tggcggagtt acagcaactg caaaatgcgc
    25501 tgactacgcc cgagcgtaat aatctggtgg cgattgtctc gctaaccgaa atggtggtgt
    25561 tgaaaccggc gttgaactct tttgggcgct gggacgcaga agatcatcgt aagagagttg
    25621 aacaactgat tacccgcatg aaagagtatg gccagttgcg ttttcgcgtt tcgctgggca
    25681 actattttac cggtcctggc agtattgccc gatcctatcg tacggcgaaa acgacgatgg
    25741 tagtggggaa acagcggatg ccagaaagtc gctgctattt ttatcaggat ctgatgttac
    25801 ctgtgttact cgacagtttg cgtggcgact ggcaggccaa cgaactggcg cgaccgctgg
    25861 cgcggctgaa agcgatggat aataacggct tgctgcgacg tacgctggcg gcgtggtttc
    25921 gccataacgt gcagccgctg gcaacgtcaa aggcgctgtt tattcatcgt aataccctgg
    25981 agtatcggct taatcgtata tcggaactca caggcttaga tctggggaat tttgatgaca
    26041 ggttattgtt gtatgtggcg ttacaactgg atgaacagcg atagggtaaa aatagtaaat
    26101 gccgggaaat atgctcgtct taattcaagt tgcatgtacg gcgactcgaa ttattcagcg
    26161 tatactttac ccggctggca gaatggcaat atgcaaaatt atttacgtgt cagcttttct
    26221 aaatctgctt ctatctcgct gattttattg gtcaccactg actccaggtg acgcaagtcg
    26281 tcgagaattt tacgcttaag gtcaacttcg ctacgatcac gctggcagat ttgatcaagc
    26341 tcatcaatga tataacgaag attagggctg atttcctgaa cttctttata tccctggcca
    26401 ataccatcgg cgaccaccgt tttgcgctga cgcggatatt taaacttcac actcttggcg
    26461 aaaaactcgc ctttatcttt ctgaaaatag attttcaaaa tatcgttatt ggcttcctgc
    26521 cggaggctgt aacgatcaat ttcctcagga ttggtaatgc ccaaactttt cagattgtcg
    26581 tacatagttg tacctcaaaa gagtcagtaa ttgtgcttat cttagcattt cgcctggcgg
    26641 tttagctcga cggagatcgc aggaagcctt ttcttccaga cttttatcat ggcctaaaaa
    26701 tcaaaaaatt acctgcttta ttctgataag agaattcgct ttctacacca gttccaggat
    26761 tgtcacttcc gggcggcaat tcagacgcaa gccatacaaa cttccgacac cccgagtcgt
    26821 gtaaatttgt cgctcaccaa aggcatttaa accggcggcg tagcgtttat cttctatggg
    26881 cgcaaaaggc tcaccaacca acggtatgcg taactgcccg ccgtgggtgt gaccacagag
    26941 catcagatcc cacttttcat tacgcatgac ttctttgctg tcgggattat gcgccagcac
    27001 cagtcgtggc agatttgctt cgctggcggg aggcggtttg cattgtccgg tccataaatc
    27061 acccgtgcca accagttcaa attgctgctt ttgagtggtg attagcatgg cttcgttaaa
    27121 taacaccgtg atccccgccg attttaatgc ctcgccaatt aagcgatttt tctctgtacc
    27181 aacagggcga tcgtggttgc cgtaacaggc aaatgtcggc gcacattcgg caagggagga
    27241 gagtacgtca ctaaacgccg aaaaattcag ctgcatatca aataatacgt aatcgccgcc
    27301 cagcaatatc aaatcgggct tttgtgctac gccaagagca attgcgtcag aaatcaggct
    27361 taaaggaaca aaacgagagt aatggagatc tgccagaaaa aggattttga aaggagcagc
    27421 gttgtcttta aaaaaggcga ggcggtgacg gattaattca agccagccag gttcccaata
    27481 atgcatatag ctgaggcttg atccggcagc taacgtcgca gcggctgcct gtaaaaaacg
    27541 cctgcgtgtg atcatcatcc atccctgcaa aaaatcgggc agcgtcatgc tgcccgtgta
    27601 ctgattttta gtcgatggta cgcagcagtt cgttaatgcc gactttgccg cgagttttcg
    27661 cgtcaacttt cttaacgata actgcacaat aaaggctgta tttgccatct ttcgacggca
    27721 ggttgccaga aacaaccacc gaaccagccg gaacgcgacc gtagtggatt tcgccggttt
    27781 cacggtcgta aatacgggtg ctctgaccaa tgtatacgcc catagagatg acggaacctt
    27841 cttcgacaat caccccttca accacttcag agcgtgcacc gatgaagcaa ttatcttcaa
    27901 taatggtcgg gttagcctgc agcggctcca gcacgccgcc gatgcctaca ccgccagaca
    27961 gatggacgtt tttaccaatt tgtgcacagg aaccgacagt ggcccaggta tcaaccatag
    28021 tgccttcatc aacatatgcg ccgatgttga cgtaagacgg catcagcacg gtgttacggg
    28081 caataaatgc accctgacgt accgccgctg gtggcacaac gcggaagcct tctttctgaa
    28141 aacgcgcttc gtcgtagtcg gcgaatttca tcggcacttt atcgaagtag cggctttctg
    28201 ccccttcgat cacctgatta tcattaatac ggaaagagag cagcaccgct tttttcaacc
    28261 actgatgcgt cacccactga ccgtcaattt tttccgctac acgcagtgcg ccggaatcca
    28321 gcagggcgat cacctgattt accgcttcgc gggttacggt gtctgcattg gctggcgtga
    28381 tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg taactgctgc attgttaaac
    28441 tcttttcata tcagtaaaca catcaccctt tatcgtttgg attgagggcc tctgtcaacc
    28501 gctgatgcac ttcctgctgc aactcgttat taagcgcacg ccggtcagcg gtggcaatta
    28561 tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg ggcgccatga agcgaaattc
    28621 ccagatcggc aaaaattttc ccgactcgcg ccagcagtcc aggttggtcg agggcgatca
    28681 gttcgaggaa cgatttgcgg tcggtatggg tcggcaaaaa cgtcacttcg gtttcaacag
    28741 taaaatggcg caatttggcg ggttggcgac ggggctgcgg tggctgccag ctactttgcg
    28801 tcagcacctg ctccagacca aaccgaataa cctcatgacg atctgcggat agcgggctac
    28861 catcgggttc cagcacgatg aaggtatcca tcgccatacc gtcgcgagtg gtgaaaattt
    28921 gtgcgtcgtg aacacttaaa ttgcggcggt ctaattcggc acagacggcg gcaaacagat
    28981 aggggcggtc cgggctccag ataaaaatct cggtgcctcc acgcgtagcc tgcggactaa
    29041 gcaataccag cggtttgctt aaatcatgct gtaataaatg gcgcgcatgc caggccagtt
    29101 gatttgggct atggcggaca aaatagttag cacgacagcg tgaccaaatt tggtgcagcg
    29161 cctcttcgtc gatgttatcc atgcgcagta gtgccagcgc ctggagttgg tgatggcgaa
    29221 cccgttcgcg catatccggc gtgttttgca ttccgcgtcg tagctgcttt tcggtggcga
    29281 aatagagttc gcgcaacaga ctttgcttcc agctattcca cagcgtttcg ttggtggcgc
    29341 atatatcggc cactgtcagg cataccagat aacgaagtcg gttttctgtt tgcacttctt
    29401 cggcaaactg cttgatcact tccgggtcct gaatatcgcg gcgttgggcg gtcaccgaca
    29461 tcagcaagtg ctggcgaacc agccacgcca ccagctgtgt ttcgcgcgag ttaagcccgt
    29521 ggagttcggc aaaatgcacc acgtcttgtg caccgagaat ggagtggtcg ccgccacgcc
    29581 ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag ctcagttgac ggcaggcgcg
    29641 gccagacgtc tacacacaac ggatggcgct ggcgcgtttc ttcactggca aaactctcca
    29701 gtttcagcat cacgcgaata gtatgttcat ccaccgtgta ggcgtggaac aggtcaaact
    29761 gcatctgccc gacgatatgc gaccattggg gcatatacgc gccgagcacg ctatgacgat
    29821 gcatcggtaa tagtccgcga cgcaccgcac cagggtgacg cagaatgctc aaaaacagtt
    29881 ttcgcgcttc cggaatatta cacagcggtt gttgcaaatg gcgacgggcg tggcgtaact
    29941 ggcgcagcgt ggtagagtaa atgccggtga tcgcactgtt gcgcaccatg gtgtagaaca
    30001 tacgcaagat ggcttccggc tggcgcataa atagtgtttc atcacgcagg tcgatcagcg
    30061 ttccgcgtag ctgaaactca tcgtcgattg gacgtggttt ttcgtcggcg ggaagggcga
    30121 ggatggcttc atcgaacagt tgcagcagca tctggttgag ttcactgacg cggcgtgtaa
    30181 cgcggaagta atccttcatc atccgctcga ccggttcgtt accttcgcca ctgtaattca
    30241 gacgctgggc gacgctaagc tggcgatcaa acagcaggcg attatcgtaa cggctgacga
    30301 ccagatgcag ggcaaagcga atacgccaca atatatgcag acattcgttt aattccgctc
    30361 gctccgctga ggttaagaag ccaaacccga ccatttcatc cagcgatgtt gcgccaaaat
    30421 gacggcgggc cacccattgc agagtgtgaa tatcgcgcaa gccgccagga ctacttttga
    30481 tgtctggttc aaggttgtag ctggtgccgt ggtaacgctg atggcgctgg ttctgttctt
    30541 caactttcgc tgcgtagaac ttgtcggaag gccagaatcc ttcgctaaaa atatgttttt
    30601 gcagttcgag gaagagcgct acatcgccaa ttaataagcg ggattcgatt aaattggtgg
    30661 cgacggttaa atccgataac ccttccagca tgcactcttc aagcgtgcgc acgctatgac
    30721 cgacttccag ctttacatcc cagagcagcg ttaacagctc gcccactttt tgcgcctgat
    30781 catccgggag ctttttacgg cttaaaatca gtaaatcgat gtcggaaagt ggatgcagct
    30841 cgccacgacc ataaccaccg acggcaacca atgccaggtc ggcaatctgg ctgaatcccg
    30901 cttcaatcca taatcgttgc aggagttggt cgataaactc ggtgcgtgcc tcaatcaact
    30961 gttctgcgga gatcccattg tcaaaggcat cacccagcca acgctggaaa gtatcgatat
    31021 gggcttttat cccacagacg gttaattcat cacggggcca ggcgcatgga ttttgcggtt
    31081 gaccgggcag ggtggggaga gcggtgtttg cgtactgttc tggaagggta ttcattgtgc
    31141 gccacccata agattaaatt atcgcattaa aaaagccggc attcgccggc ttcatcttat
    31201 tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc gtagcgtcag aatttcgcag
    31261 ccgttatcag tcaccacaat agtatgctca tattgtgcag acaagctgcg atctttggtt
    31321 tttaccgtcc agccatcttt catggtgcgg atctcttttt tacccgcgtt gaccattggc
    31381 tcgatggtga acgtcatccc aggtttcagt acgacgttgg tttcacggga gtcatagtgc
    31441 agcacctgcg gttcttcgtg gaagccgcga ccaataccgt gtccacaata ttcacgaacg
    31501 acggagaagc cttctgcttc gacaaatttc tgaattgccg caccgatttc gcgcagatta
    31561 atgccaggtt ttaccatgcg tagcgccagg tacaggcttt cttgcgtgat gcggcacaga
    31621 cgttcgccca tgatggtcgg cttaccgacg ataaacattt tcgaggtatc gccgtggaaa
    31681 ccatctttga ttacggtgac atcaatgtta acgatatcgc catctttcag cagcttggcg
    31741 tcgtccggga taccgtggca caccacttca ttaatagaaa tgcaaacgga tttcggatag
    31801 ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat taacaatgta atcattacag
    31861 atgcgatcca gctcgccggt gctgacgccc ggtttaacat acggttcgat catctccagc
    31921 acttcggcag ccagtcggcc agcgacgcgc attttttcga tatcttctgg ggtcttgatt
    31981 gagatagcca ttaattctgt ccatcagcgt cggtgatacc gacaatatat atgtaagtgc
    32041 cgtcaatggt atcacacccg ggcaaattga gaatcattct gaatttcgcc aaacgtgcca
    32101 ctgaaggttt tctataatag aaaattcaac gtctgatgct gtacacagcg ccaacaatta
    32161 ttggtgtcca cgacgtattt gtggtataaa gcgcgccgga cttccgatcc atttcgtata
    32221 cacagactgg acggaagcga caatctcact ttgtgtaaca acacacacgt atcggcacat
    32281 attccggggt gccctttggg gtcggtaata tgggatacgt ggaggcataa ccccaacttt
    32341 tatatagagg ttttaatcat ggcaactgtt tccatgcgcg acatgctcaa ggctggtgtt
    32401 cacttcggtc accagacccg ttactggaac ccgaaaatga agccgttcat cttcggtgcg
    32461 cgtaacaaag ttcacatcat caaccttgag aaaactgtac cgatgttcaa cgaagctctg
    32521 gctgaactga acaagattgc ttctcgcaaa ggtaaaatcc ttttcgttgg tactaaacgc
    32581 gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg accagttctt cgtgaaccat
    32641 cgctggctgg gcggtatgct gactaactgg aaaaccgttc gtcagtccat caaacgtctg
    32701 aaagacctgg aaactcagtc tcaggacggt actttcgaca agctgaccaa gaaagaagcg
    32761 ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc tgggcggtat caaagacatg
    32821 ggcggtctgc cggacgctct gtttgtaatc gatgctgacc acgaacacat tgctatcaaa
    32881 gaagcaaaca acctgggtat tccggtattt gctatcgttg ataccaactc tgatccggac
    32941 ggtgttgact tcgttatccc gggtaacgac gacgcaatcc gtgctgtgac cctgtacctg
    33001 ggcgctgttg ctgcaaccgt acgtgaaggc cgttctcagg atctggcttc ccaggcggaa
    33061 gaaagcttcg tagaagctga gtaataaggc ttgataactc ccccaaaata gttcgagttg
    33121 cagaaaggcg gcaagctcga gaattcccgg gagcttacat cagtaagtga ccgggatgag
    33181 cgagcgaaga taacgcatct gcggcgcgaa atatgaaggg ggagagccct tatagaccag
    33241 gtagtacacg tttggttagg gggcctgcat atggccccct ttttcacttt tatatctgtg
    33301 cggtttaatg ccgggcagat cacatctccg aggattttag aatggctgaa attaccgcat
    33361 ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat gatggattgc aaaaaagcac
    33421 tgactgaagc taacggcgac atcgagctgg caatcgaaaa catgcgtaag tccggtgcta
    33481 ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg cgtgatcaaa accaaaatcg
    33541 acggcaacta cggcatcatt ctggaagtta actgccagac tgacttcgtt gcaaaagacg
    33601 ctggtttcca ggcgttcgca gacaaagttc tggacgcagc tgttgctggc aaaatcactg
    33661 acgttgaagt actgaaagca cagttcgaag aagaacgtgt tgcgctggta gcgaaaattg
    33721 gtgaaaacat caacattcgc cgcgttgctg cgctggaagg cgacgttctg ggttcttatc
    33781 agcacggtgc gcgtatcggt gttctggttg ctgctaaagg cgctgacgaa gagctggtta
    33841 aacacatcgc tatgcacgtt gctgcaagca agccagaatt catcaaacca gaagacgtat
    33901 ccgctgaagt ggtagaaaaa gaataccagg tacagctgga tatcgcgatg cagtctggta
    33961 agccgaaaga aatcgcagag aaaatggttg aaggccgcat gaagaaattc accggcgaag
    34021 tttctctgac cggtcagccg ttcgttatgg aaccaagcaa aactgttggt cagctgctga
    34081 aagagcataa cgctgaagtg actggcttca tccgcttcga agtgggtgaa ggcatcgaga
    34141 aagttgagac tgactttgca gcagaagttg ctgcgatgtc caagcagtct taattatcga
    34201 aaaggagccg cctgagggcg gcttcttttt gtgcccatct tgtaaattca gctaaccctt
    34261 gtggggctgc gctgaaaagc gacgtacaat gtcgctagta ttaattcatt tcaatcgttg
    34321 acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc gtctataaac gcattctgct
    34381 taagttgagt ggcgaagctc tgcagggcac tgaaggcttc ggtattgatg caagcatact
    34441 ggatcgcatg gctcaggaaa tcaaagaact ggttgaactg ggtattcagg ttggtgtggt
    34501 gattggtggg ggtaacctgt tccgtggcgc tggtctggcg aaagcgggta tgaaccgcgt
    34561 tgtgggcgac cacatgggga tgctggcgac cgtaatgaac ggcctggcaa tgcgtgatgc
    34621 actgcaccgc gcctatgtga acgctcgtct gatgtccgct attccattga atggcgtgtg
    34681 cgacagctac agctgggcag aagctatcag cctgttgcgc aacaaccgtg tggtgatcct
    34741 ctccgccggt acaggtaacc cgttctttac caccgactca gcagcttgcc tgcgtggtat
    34801 cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt gacggcgtgt ttactgctga
    34861 tccggcgaaa gatccaaccg caaccatgta cgagcaactg acttacagcg aagtgctgga
    34921 aaaagagctg aaagtcatgg acctggcggc cttcacgctt gctcgtgacc ataaattacc
    34981 gattcgtgtt ttcaacatga acaaaccggg tgcgctgcgc cgtgtggtaa tgggtgaaaa
    35041 agaagggact ttaatcacgg aataattccc gtgatggata aataagggta agattccgcg
    35101 taagtatcgc gggggcgtaa gtctggttat aaggcgttat tgttgcaggc agtttggtca
    35161 cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga ggatggcgag cactgcccgg
    35221 ggccaaaatg gcaaataaaa tagcctaata atccagacga ttacccgtaa tatgtttaat
    35281 cagggctata cttagcacac ttccactgtg tgtgactgtc tggtctgact gagacaagtt
    35341 ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat gctgaagtac gcatggataa
    35401 atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc acgggtcgtg cttctcccag
    35461 cctgctggat ggcattgtcg tggaatatta cggcacgccg acgccgctgc gtcagctggc
    35521 aagcgtaacg gtagaagatt cccgtactct gaaaatcaac gtgtttgatc gttcaatgtc
    35581 tccggccgtt gaaaaagcga ttatggcgtc cgatcttggc ctgaacccga actctgcggg
    35641 tagcgacatc cgtgttccgc tgccgccgct gacggaagaa cgtcgtaaag atctgaccaa
    35701 aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta cgtaatgtgc gtcgtgacgc
    35761 gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc agcgaagacg acgatcgccg
    35821 ttctcaggac gatgtacaga aactgactga tgctgcaatc aagaaaattg aagcggcgct
    35881 ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt gaacgacaaa aacgccgctc
    35941 agtagatcct tgcggatcgg ctggcggcgt tttgcttttt attctgtctc aactctggat
    36001 gtttcatgaa gcaactcacc attctgggct cgaccggctc gattggttgc agcacgctgg
    36061 acgtggtgcg ccataatccc gaacacttcc gcgtagttgc gctggtggca ggcaaaaatg
    36121 tcactcgcat ggtagaacag tgcctggaat tctctccccg ctatgctgta atggatgatg
    36181 aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca gggtagccgc accgaagtct
    36241 taagtgggca acaagccgct tgcgatatgg cagcgcttga ggatgttgat caggtgatgg
    36301 cagccattgt tggcgctgct gggctgttac ctacgcttgc tgcgatccgc gcgggtaaaa
    36361 ccattttgct ggccaataaa gaatcactgg ttacctgcgg acgtctgttt atggacgccg
    36421 taaagcagag caaagcgcaa ttgttaccgg tcgatagcga acataacgcc atttttcaga
    36481 gtttaccgca acctatccag cataatctgg gatacgctga ccttgagcaa aatggcgtgg
    36541 tgtccatttt acttaccggg tctggtggcc ctttccgtga gacgccattg cgcgatttgg
    36601 caacaatgac gcccgatcaa gcctgccgtc atccgaactg gtcgatggga cgtaaaattt
    36661 ctgtcgattc ggctaccatg atgaacaaag gtctggaata cattgaagcg cgttggctgt
    36721 ttaacgccag cgccagccag atggaagtgc tgattcaccc gcagtcagtg attcactcaa
    36781 tggtgcgcta tcaggacggc agtgttctgg cgcagctggg ggaaccggat atgcgtacgc
    36841 caattgccca caccatggca tggccgaatc gcgtgaactc tggcgtgaag ccgctcgatt
    36901 tttgcaaact aagtgcgttg acatttgccg caccggatta tgatcgttat ccatgcctga
    36961 aactggcgat ggaggcgttc gaacaaggcc aggcagcgac gacagcattg aatgccgcaa
    37021 acgaaatcac cgtagctgct tttcttgcgc aacaaatccg ctttacggat atcgccgcgt
    37081 tgaatttatc cgtactggaa aaaatggata tgcgcgaacc acaatgtgtg gacgatgtgt
    37141 tatctgttga tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt ctcgcaagct
    37201 gaggataatc cggctacaga gagtcgcgct atttgttagc gtagggcttc agtgatatag
    37261 tctgcgccat ctgatcgtaa gtagttggct ttataaggtc aggtatgccg tggttttaca
    37321 cggctttttt ttgtataggc ttcagtattc ctgagtaccg taaaccctgt cagggaataa
    37381 gaaacgcgtg atgttgtctg ctactcaacc acttagcgaa aaattgccag cgcatggctg
    37441 tcgtcatgtt gcgatcatta tggacggcaa tggtcgctgg gcaaaaaagc aagggaagat
    37501 tcgtgccttt gggcataaag ccggggcaaa atccgtccgc cgggctgtct cttttgcggc
    37561 caacaacggt attgaggcgt taacgctgta tgcctttagt agtgaaaact ggaaccgacc
    37621 agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg gcgctcgata gcgaagtaaa
    37681 aagtctgcac cgacataacg tgcgtctgcg tattattggc gataccagtc gctttaactc
    37741 gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca gccgggaata ccggtctgac
    37801 gctgaatatt gcggcgaact acggtggacg ttgggatata gtccagggag tcaggcaact
    37861 ggctgaaaag gtgcagcaag gaaacctgca accagatcag atagatgaag agatgctaaa
    37921 ccagcatgtc tgtatgcatg aactggcccc tgtagattta gtaattagga ctggggggga
    37981 gcatcgcatt agtaactttt tgctttggca aattgcctat gccgaacttt actttacaga
    38041 tgttctctgg cccgatttcg atgaacaaga ctttgaaggg gcgttaaatg cctttgctaa
    38101 tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa acagcctgat gggggtcgct
    38161 tttgctgaag tatcgcctga tatctgcttt tgtgttaata cccgttgtca tcgcggcgtt
    38221 gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg gtggtctgca tgctggcagc
    38281 gtgggaatgg ggacagctta gcggttttac cactcgttcg cagcgagtat ggttggcggt
    38341 gttatgcggg ttattgttgg cgctgatgct ttttctgttg ccggaatatc accgaaatat
    38401 tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg ggttggtgga ttgtcgcgct
    38461 attgctggtg ctgttttacc ctggttccgc agcaatctgg cgtaactcta aaacattgcg
    38521 ccttattttt ggcgtgctaa ccattgttcc cttcttctgg ggcatgctgg cgttacgggc
    38581 ctggcactac gacgagaatc attacagtgg cgcaatatgg ctgctctatg tcatgatcct
    38641 ggtatggggc gctgactctg gcgcatatat gtttggcaaa ttgtttggta aacataagct
    38701 ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt ataggtggac tcgcgaccgc
    38761 agcggtaatc tcatggggtt atggcatgtg ggcgaatctc gacgtcgctc ccgtcacctt
    38821 actcatttgc tctattgtcg cagcgttagc ctcagtgctc ggcgatctga ccgagagtat
    38881 gtttaagcgt gaagcaggaa ttaaggacag cggtcattta attccaggac acggtggtat
    38941 tttagatcgt attgatagcc tgacggctgc ggtaccggtc tttgcttgct tgttgttact
    39001 ggtattcagg acgctttaac ggaaggtaat atgctgagtt ttctctggga tttggcttcg
    39061 ttcatcgttg cactgggtgt acttatcacc gtgcatgaat ttggtcattt ctgggttgcc
    39121 cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt ttggtaaggc gctctggcgg
    39181 cgaactgata agctcggcac cgaatatgtt atcgccctga tcccgctggg cggttatgtc
    39241 aaaatgctgg atgagcgcgc agaaccggtc gttccggaac tccgccacca tgccttcaat
    39301 aataaatctg tcggccaacg agcggcgatt attgccgcag gtccggttgc aaacttcatt
    39361 tttgctatct ttgcctactg gctggttttt attattggtg tgcctggcgt acgtccggtg
    39421 gttggcgaaa tagcagccaa ttcgatagct gcggaagcac aaattgcacc aggtacggaa
    39481 ctaaaagccg tagatggtat cgaaacgcct gattgggatg ccgtgcgttt gcagttggtc
    39541 gataaaattg gcgatgaaag caccaccatt acggtagcgc catttggcag cgaccaacgg
    39601 cgggatgtaa agctcgattt acgtcactgg gcgtttgagc ctgataaaga agatccggta
    39661 acttcgctgg ggattcgtcc tcgtgggccg caaattgaac ctgtactgga aaatgtgcag
    39721 ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg acaggatcgt taaagtcgat
    39781 ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg tccgggataa cccgggtaaa
    39841 tccttagcgt tagaaatcga aaggcagggg agtcccttgt ctttgacatt aatcccggag
    39901 agtaaaccgg gtaatggtaa agcgattggt tttgtcggta ttgagccgaa agtcattcct
    39961 ttgccagatg agtataaagt tgtacgccag tatgggccgt tcaacgccat cgtcgaagcc
    40021 acggacaaaa cgtggcagct gatgaagctg acggtcagta tgctgggaaa attgatcacc
    40081 ggtgatgtga aactgaacaa cctcagtggg ccgatctcta tcgccaaggg ggctgggatg
    40141 acagcggaac tcggggttgt ttattacctg ccgtttcttg cgcttattag cgtgaactta
    40201 gggataatta acctgtttcc gttgcccgta cttgacgggg ggcatctgct gttccttgcg
    40261 atcgaaaaga tcaagggcgg accggtatcc gagcgggttc aagacttttg ttatcgcatt
    40321 ggctcgattt tgctggtgct gttaatgggg cttgcacttt tcaatgattt ctctcggtta
    40381 tgagagttag ttaggaagaa cgcataataa cgatggcgat gaaaaagttg ctcatagcgt
    40441 cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg gttcgtagtg aaagatattc
    40501 atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct cctcagtatg ccggtgcgca
    40561 caggcgacac ggttaatgat gaagatatca gtaataccat tcgcgctctg tttgctaccg
    40621 gcaactttga ggatgttcgc gtccttcgtg atggtgatac ccttctggtt caggtaaaag
    40681 aacgtccgac cattgccagc attactttct ccggtaacaa atcggtgaaa gatgacatgc
    40741 tgaagcaaaa cctcgaggct tctggcgtgc gtgtgggcga atccctcgat cgcaccacca
    40801 ttgccgatat cgagaaaggt ctggaagact tctactacag cgtcggtaaa tatagcgcca
    40861 gcgttaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt tgacctaaaa ctggtgttcc
    40921 aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt tggtaaccat gctttcacca
    40981 ccgacgaact gatctctcat ttccaactgc gtgacgaagt gccgtggtgg aacgtggtag
    41041 gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct tgaaaccctg cgcagttact
    41101 atctggatcg cggttatgcc cgtttcaaca tcgactctac ccaggtcagt ctgacgcctg
    41161 ataaaaaagg tatttacgtc acggtgaaca tcaccgaagg cgatcagtac aagctttctg
    41221 gcgttgaagt gagcggcaac cttgccgggc actccgctga aattgagcag ctgactaaga
    41281 tcgagccggg cgagctgtat aacggcacca aagtgaccaa aatggaagat gacattaaaa
    41341 agcttcttgg tcgctatggt tatgcctatc cgcgcgtaca gtcgatgcct gaaattaacg
    41401 atgccgacaa aaccgttaaa ttacgtgtga acgttgatgc gggtaaccgt ttctacgtgc
    41461 gtaagatccg ttttgaaggt aacgatacct cgaaagatgc cgtcctgcgt cgcgaaatgc
    41521 gtcagatgga aggtgcatgg ctggggagcg atctggtcga tcagggtaag gagcgtctga
    41581 atcgtctggg cttctttgaa actgtcgata ccgataccca acgcgttccg ggtagcccgg
    41641 accaggttga tgtagtctac aaggtgaaag aacgtaacac cggtagcttc aacttcggga
    41701 ttggttacgg tactgaaagt ggcgtgagct tccaggctgg tgtgcaacag gataactggt
    41761 taggtacggg ttatgctgtt ggtatcaacg ggaccaaaaa cgattaccag acctatgctg
    41821 aactgtcggt aaccaacccg tacttcaccg tagatggtgt aagcctcggt ggtcgtctct
    41881 tctataatga cttccaggca gatgacgccg acctgtccga ctataccaac aagagttatg
    41941 gtacagacgt gacgttgggc ttcccgatca acgaatataa ctcgctgcgt gcaggtctgg
    42001 gttatgtaca taactccctg tccaacatgc agcctcaggt tgcgatgtgg cgttatctgt
    42061 actctatggg tgaacatccg agcacctctg atcaggataa cagcttcaaa acggacgact
    42121 tcacgttcaa ctatggttgg acctataaca agcttgaccg tggttacttc ccgacagatg
    42181 gttcacgtgt caacctgacc ggtaaagtga ccattccggg atcggataac gaatactaca
    42241 aagtgacgtt agacacggcg acttatgtgc cgattgatga cgatcacaaa tgggttgttc
    42301 tgggtcgtac ccgctggggt tatggtgatg gtttaggcgg caaagagatg ccgttctatg
    42361 agaacttcta tgccggtggt tccagcaccg tgcgtggctt ccagtccaat accattggtc
    42421 cgaaagcagt ttacttcccg catcaggcca gtaattatga tccggactat gattacgaat
    42481 gtgcgactca ggacggcgcg aaagatctgt gtaaatcgga tgatgctgta ggcggtaacg
    42541 ccatggcggt tgccagcctc gagttcatca ccccgacgcc gtttattagc gataagtatg
    42601 ctaactcggt tcgtacttcc ttcttctggg atatgggtac cgtttgggat acaaactggg
    42661 attccagcca atattctgga tatccggact atagtgatcc aagcaatatc cgtatgtctg
    42721 cgggtatcgc attacaatgg atgtccccat tggggccgtt ggtgttctcc tacgcccagc
    42781 cgttcaaaaa gtacgatgga gacaaggcag aacagttcca gtttaacatc ggtaaaacct
    42841 ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc gatgacttta ggcgatcaat
    42901 ataagatcgc cgggccacgc aaagaactgc accctccggt gcaaatggga tggtaaggag
    42961 tttattgtga aaaagtggtt attagctgca ggtctcggtt tagcactggc aacttctgct
    43021 caggcggctg acaaaattgc aatcgtcaat atgggcagcc tgttccagca ggtagcgcag
    43081 aaaaccggag tttctaacac gctggaaaat gagttcaaag gccgtgccag cgaactgcag
    43141 cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc agtccatgaa agcgggcagc
    43201 gatcgcacca aactggaaaa agacgtgatg gcccagcgcc agacttttgc tcagaaagcg
    43261 caggcttttg agcaggatcg cgcacgtcgt tccaacgaag aacgcggcaa actggttact
    43321 cgtatccaga ctgctgtgaa atccgttgcc aacagccagg atatcgatct ggttgttgat
    43381 gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca tcactgccga cgtactgaaa
    43441 caggttaaat aagtaatgcc ttcaattcga ctggctgatt tagcgcagca gttggatgca
    43501 gaactacacg gtgatggcga tatcgtcatc accggcgttg cgtccatgca atctgcacaa
    43561 acaggtcaca tcacgttcat ggttaaccca aaataccgtg agcatttagg cttgtgccag
    43621 gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg cgaaaagtgc cgcgctggta
    43681 gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa ttttagatac cacgccgcag
    43741 cccgcgcaga acattgcacc cagtgcggtg attgacgcga cggcgaagct gggtaacaac
    43801 gtatcgattg gcgctaacgc ggtgattgag tccggcgttg aactgggcga taacgtgatt
    43861 atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg gtgcaggttc gcgtctctgg
    43921 gcgaacgtaa ccatttacca tgagatccag atcggtcaga attgcctgat ccagtccgga
    43981 acagtggtag gcgcagacgg ctttggttat gccaacgatc gtggtaactg ggtgaagatc
    44041 ccacagattg gtcgcgtaat tattggcgat cgcgtggaga tcggtgcctg cactaccatt
    44101 gatcgtggcg cgctggatga cactgttatt ggcaatggtg tgatcattga taaccagtgc
    44161 cagattgcac ataacgtcgt gattggcgac aatacggcag ttgccggtgg cgtcattatg
    44221 gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg gagccagcgt aatcaacggg
    44281 catatggaaa tatgcgacaa agtgacggtt acgggcatgg gtatggtgat gcgtcccatc
    44341 actgaaccag gcgtctattc ctcaggcatt ccgctgcaac ccaacaaagt ctggcgcaaa
    44401 accgctgcac tggtgatgaa cattgatgac atgagcaagc gtctgaaatc gcttgagcgc
    44461 aaggttaatc aacaagacta acgttccatc ttttgttcgc caaactttac ggcctgtctc
    44521 attcttacga ttgcggcagg ccgtgttatt attgtcgttt cttatatttt gacaggaaga
    44581 gtatcttgac tactaacact catactctgc agattgaaga gattttagaa cttctgccgc
    44641 accgtttccc gttcttactg gtggatcgcg tgctggattt tgaagaaggt cgttttctgc
    44701 gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca gggccatttc cctggaaaac
    44761 cgattttccc gggtgtgctg attctggaag caatggcaca ggcaacaggt attctggcgt
    44821 ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta cttcgccggt attgacgaag
    44881 cgcgctttaa gcgcccggtc gtgcctggcg atcaaatgat catggaagtc actttcgaaa
    44941 aaacgcgccg cggcctgacc cgttttaaag gggttgctct ggtcgacggt aaagtagttt
    45001 gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg atacgtgatt gataaatccg
    45061 cctttgtgca tccaaccgcc attgtggaag agggcgcgtc gattggcgcg aacgctcaca
    45121 ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg tgagggtacc gtactgaaat
    45181 ctcacgttgt cgtgaatggt catactaaaa ttggccgcga caatgagatt tatcagttcg
    45241 cctccatcgg cgaagtcaac caggatctta aatatgctgg cgaaccgacc cgtgtggaaa
    45301 tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca tcgtggcaca gtccagggcg
    45361 gtggattgac gaaggtgggc agcgacaact tactgatgat caacgcgcac attgcgcacg
    45421 attgtacggt aggtaaccgc tgtattctcg ccaacaacgc aacgctggcg ggtcacgtat
    45481 cggttgacga cttcgcgatc atcggcggca tgaccgcagt ccatcagttc tgcatcattg
    45541 gtgcgcacgt gatggttggc ggctgctccg gtgtggcgca ggacgtccct ccttatgtca
    45601 ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat cgaagggctg aagcgccgcg
    45661 gattcagccg tgaggcgatt accgctatcc gcaatgcgta taagctgatt tatcgtagcg
    45721 gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact ggcggaaaca tatccggaag
    45781 tgaaagcctt taccgatttc tttgcacgct caacgcgcgg tctgattcgt taatgactga
    45841 acagcgtcca ttaacgattg ccctggtcgc cggagaaacc tccggcgata tcctgggggc
    45901 cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc cgctttgttg gtgttgccgg
    45961 gccacgaatg caggctgaag gctgcgaagc ctggtacgaa atggaagaac tggcggtgat
    46021 gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg catattcgtg ccgatctgac
    46081 aacgcgtttt ggcgaactga agccagatgt ttttgtcggt attgatgcgc ctgacttcaa
    46141 tattaccctt gaaggtaacc ttaaaaagca gggtatcaaa accattcatt atgtcagtcc
    46201 gtccgtctgg gcgtggcgac agaaacgcgt tttcaaaata ggcagagcca ccgatctggt
    46261 gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa tataacgtgc cgtgccgttt
    46321 tatcggtcac accatggctg atgccatgcc gttagatcca gataaaaacg ccgcgcgtga
    46381 tgtgctgggg atccctcacg atgcccactg tctggcattg ttgccgggaa gccgtggcgc
    46441 ggaagtggag atgcttagcg ccgatttcct gaaaacggcc cagcttttgc gccagacgta
    46501 tcctgatctg gagatcgtgg tgccgctggt aaatgccaaa cgccgtgagc aatttgaacg
    46561 catcaaagct gaagtcgcgc cagacctttc ggttcatatg ctggatggaa tgggccgtga
    46621 ggcgatggtc gccagcgatg cggcgctatt ggcgtcgggt acggctgcac tggagtgcat
    46681 gctggcgaaa tgcccgatgg tggtggggta tcgcatgaag cctttcacct tctggttggc
    46741 gaagcggctg gtgaaaaccg attatgtctc gctgccaaac ctgctggcgg gcagagagtt
    46801 agttaaagaa ttattgcagg aagagtgtga gccgcaaaaa ctggctgcgg cgctgttacc
    46861 gctgttggcg aacgggaaaa ccagccacgc gatgcacgat accttccgtg aactgcatca
    46921 gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc gttctggagt tagcacaatg
    46981 atcgaatttg tttatccgca cacgcacctg gttgcgggtg tggatgaagt cggacgcggg
    47041 ccgttagttg gcgcggtcgt caccgctgcg gtgatcctcg acccggcgcg cccgattgcc
    47101 gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc tggcgctctg cgaagagatc
    47161 aaagagaaag cgttgagctg gagtctgggc cgagcggaac ctcacgaaat cgacgaactg
    47221 aatattttac acgcgaccat gctggcgatg cagcgtgccg tcgctgggct gcatattgcg
    47281 ccggaatatg tgttgattga tggtaaccgc tgcccgaaat taccgatgcc tgcgatggct
    47341 gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg cgtctatcct ggcgaaagtg
    47401 acgcgtgacg ccgaaatggc ggcgctggat attgttttcc cgcaatatgg ttttgcccaa
    47461 cataaagggt atccaaccgc ttttcatctg gaaaaactgg ctgaatatgg cgcgactgaa
    47521 catcatcgtc gcagctttgg gcctgtcaaa cgcgtactgg gacttgcgtc ctgattcttg
    47581 tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac cacgtttcgt acacctgcgg
    47641 gtgcacagcg actactcgat gatcgatggt ctggccaaaa ccgcaccgct ggtaaaaaag
    47701 gcggcggcgt tgggtatgcc agcactggcg atcaccgatt tcaccaacct ttgcggtctg
    47761 gtgaagttct acggagcggg acatggcgca gggattaagc ctatcgtcgg ggcagatttt
    47821 aatgtccagt gcgacctgct gggtgatgag ttaacccacc tgacggtgct ggcggcgaac
    47881 aataccggct atcagaatct gacgttgctg atctcaaaag cgtatcagcg cgggtacggt
    47941 gcagctgggc cgatcatcga tcgcgactgg cttatcgaat taaacgaagg gttgatcctt
    48001 ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt tgcgtggtaa cagcgcgctg
    48061 gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg atcgctattt tctcgagctg
    48121 atccgcaccg gcaggccgga cgaagaaagc tatctgcacg ctgcggtgga actggcggaa
    48181 gcacgcggtt tgcccgttgt ggcgaccaac gacgtgcgct ttatcgacag cagcgacttt
    48241 gacgcacacg aaatccgcgt cgcgatccac gacggcttta ccctcgacga tcctaaacgc
    48301 ccgcgtaact attcgccgca gcaatatatg cgtagcgaag aggagatgtg cgagcttttt
    48361 gccgacattc ccgaagccct tgccaacacc gttgagatcg ccaaacgttg taacgtaacc
    48421 gtgcgtcttg gtgaatactt cctgccgcag ttcccgaccg gggacatgag caccgaagat
    48481 tatctggtca agcgtgcaaa agaggggctg gaagagcgtc tggccttttt attccctgac
    48541 gaggaagaac gtgttaagcg ccgcccggaa tatgacgaac gtctggagac tgaacttcag
    48601 gttatcaacc agatgggctt cccgggttac ttcctcatcg ttatggaatt tatccagtgg
    48661 tcgaaagata acggcgtacc agtagggcca ggccgtggct ccggtgcggg ttcactggtg
    48721 gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat ttgacctgct gttcgaacgt
    48781 ttccttaacc cggaacgtgt ctccatgcct gacttcgacg ttgacttctg tatggagaaa
    48841 cgcgaccagg ttatcgagca cgtagcggac atgtacggtc gtgatgcggt atcgcagatc
    48901 atcaccttcg gtacgatggc ggcgaaagcg gtgatccgcg acgtaggtcg cgtgctgggg
    48961 catccgtacg gctttgtcga tcgtatctcg aaactgatcc cgcccgatcc ggggatgacg
    49021 ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa
    49081 gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag
    49141 cacgccggtg gggtggttat cgcgccgacc aaaattaccg attttgcgcc gctttactgc
    49201 gatgaagagg gcaaacatcc ggtcacccag tttgataaaa gcgacgttga atacgccgga
    49261 ctggtgaagt tcgacttcct tggtttgcgt acgctcacca tcatcaactg ggcgctggag
    49321 atgatcaaca agcggcgggc gaagaatggc gagccgccgc tggatatcgc cgcgatcccg
    49381 ctggatgata agaaaagctt cgacatgctg caacgctcgg aaaccactgc ggtattccag
    49441 cttgaatcgc gcggcatgaa ggacctgatc aagcgtctgc aacctgactg cttcgaagat
    49501 atgatcgcac tggtggcact gttccgcccc ggtccgttgc aatcagggat ggtggataac
    49561 tttatcgacc gtaaacatgg tcgcgaagag atctcctatc cggacgtgca gtggcagcat
    49621 gaaagcctga aaccggtact ggagccaacc tacggcatca tcctgtatca ggaacaggtc
    49681 atgcagattg cccaggtgct ttctggttat accctcggtg gcgcagatat gctgcgtcgt
    49741 gcgatgggta agaaaaagcc ggaagagatg gctaagcagc gttctgtatt tgctgaaggt
    49801 gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa tcttcgacct ggtggagaaa
    49861 ttcgcgggtt acggatttaa caaatcgcac tctgcggcct atgctttggt gtcatatcaa
    49921 acgttatggc tgaaagcgca ctatccggca gagtttatgg cggcggtaat gaccgccgat
    49981 atggacaata ccgagaaggt ggtgggcctg gtggatgagt gctggcggat ggggctgaaa
    50041 atcctgccac cagatataaa ctccggtctt taccatttcc acgtcaacga cgacggcgaa
    50101 atcgtgtatg gtattggcgc gatcaaaggg gtaggtgaag gtccgattga ggccatcatc
    50161 gaagcccgta ataaaggcgg ctacttccgc gaactgtttg atctctgcgc ccgaaccgac
    50221 accaaaaagt taaaccggcg ggtgctggaa aaactgatca tgtccggggc gtttgaccgt
    50281 cttgggccac accgtgcggc gctgatgaac tcgctgggcg atgcgttaaa agcggcagat
    50341 caacacgcga aagcggaagc tatcggtcag gccgatatgt tcggcgtgct ggccgaagaa
    50401 ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt ggccggagca ggtggtatta
    50461 gatggggaac gtgaaacgtt aggtctgtac ctgacgggac accctatcaa ccagtatcta
    50521 aaagagattg agcgttatgt cggaggcgta aggctgaaag acatgcaccc gacagaacgt
    50581 ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc gggttatggt caccaagcgc
    50641 ggcaatcgta tcggtatctg cacgctggat gaccgttccg ggcggctgga agtgatgttg
    50701 tttactgacg ccctggataa ataccagcaa ttgctggaaa aagaccgcat acttatcgtc
    50761 agcggacagg tcagctttga tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg
    50821 atggatattg acgaagcccg ggaaaaatat gctcgcgggc ttgctatctc gctgacggac
    50881 aggcaaattg atgaccagct tttaaaccga ctccgtcagt ctctggaacc ccaccgctct
    50941 gggacaattc cagtacatct ctactatcag agggcggatg cacgcgcgcg gttgcgtttt
    51001 ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa acgatctccg tggcctcatt
    51061 ggttcggagc aggtggaact ggagtttgac taatacagga atactatgag tctgaatttc
    51121 cttgattttg aacagccgat tgcagagctg gaagcgaaaa tcgattctct gactgcggtt
    51181 agccgtcagg atgagaaact ggatattaac atcgatgaag aagtgcatcg tctgcgtgaa
    51241 aaaagcgtag aactgacgcg taaaatcttc gccgatctcg gtgcatggca gattgcgcaa
    51301 ctggcacgcc atccacagcg tccttatacc ctggattacg ttcgcctggc atttgatgaa
    51361 tttgacgaac tggctggcga ccgcgcgtat gcagacgata aagctatcgt cggtggtatc
    51421 gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc aaaaaggtcg tgaaaccaaa
    51481 gaaaaaattc gccgtaactt tggtatgcca gcgccagaag gttaccgtaa agcactgcgt
    51541 ctgatgcaaa tggctgaacg ctttaagatg cctatcatca cctttatcga caccccgggg
    51601 gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg aagccattgc acgcaacctg
    51661 cgtgaaatgt ctcgcctcgg cgtaccggta gtttgtacgg ttatcggtga aggtggttct
    51721 ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata tgctgcaata cagcacctat
    51781 tccgttatct cgccggaagg ttgtgcgtcc attctgtgga agagcgccga caaagcgccg
    51841 ctggcggctg aagcgatggg tatcatcgct ccgcgtctga aagagctgaa actgatcgac
    51901 tccatcatcc cggaaccgct gggcggcgct catcgtaacc cggaagcgat ggcggcatcg
    51961 ttgaaagcgc aactgctggc ggatctggcc gatctcgacg tgttaagcac tgaagattta
    52021 aaaaatcgtc gttatcagcg cctgatgagc tacggttacg cgtaattcgc aaaagttctg
    52081 aaaaagggtc acttcggtgg ccctttttta tcgccacggt ttgagcaggc tatgattaag
    52141 gaaggatttt ccaggaggaa cacatgaaca tcattgccat tatgggaccg catggcgtct
    52201 tttataaaga tgagcccatc aaagaactgg agtcggcgct ggtggcgcaa ggctttcaga
    52261 ttatctggcc acaaaacagc gttgatttgc tgaaatttat cgaacataac cctcgaattt
    52321 gcggcgtgat ttttgactgg gatgagtaca gtctcgattt atgtagcgat atcaatcagc
    52381 ttaatgaata tctcccgctt tatgccttca tcaacaccca ctcgacgatg gatgtcagcg
    52441 tgcaggatat gcggatggcg ctctggtttt ttgaatatgc gctggggcag gcggaagata
    52501 tcgccattcg tatgcgtcag tacaccgacg aatatcttga taacattaca ccgccgttca
    52561 cgaaagcctt gtttacctac gtcaaagagc ggaagtacac cttttgtacg ccgggtcata
    52621 tgggcggcac cgcatatcaa aaaagcccgg ttggctgtct gttttatgat tttttcggcg
    52681 ggaatactct taaggctgat gtctctattt cggttaccga gcttggttcg ttgctcgacc
    52741 acaccgggcc acacctggaa gcggaagagt acatcgcgcg gacttttggc gcggaacaga
    52801 gttatatcgt taccaacgga acatcgacat cgaacaaaat tgtgggtatg tacgccgcgc
    52861 catccggcag tacgctgtta attgaccgca attgtcataa atcgctggcg catctgttga
    52921 tgatgaacga tgtagtgcca gtctggctga aaccgacgcg taatgcgttg gggattcttg
    52981 gtgggatccc gcgccgtgaa tttactcgcg acagcatcga agagaaagtc gctgctacca
    53041 cgcaagcaca atggccggtt catgcggtga ttaccaactc cacctatgat ggcttgctct
    53101 acaacaccga ctggatcaaa cagacgctgg atgtcccgtc gattcacttc gattctgcct
    53161 gggtgccgta cacccatttt catccgatct accaaggtaa aagtggtatg agcggcgagc
    53221 gtgttgcggg aaaagtgatc ttcgaaacgc aatcgaccca caaaatgctg gcggcgttat
    53281 cgcaggcttc gctgatccac attaaaggcg agtatgacga agaggcgttt aacgaagcct
    53341 ttatgatgca taccaccacc tcgcccagtt atcccattgt tgcttcggtt gagacggcag
    53401 cggcgatgct gcgtggtaat ccgggcaaac ggctgattaa ccgttcagta gaacgagcgc
    53461 tgcattttcg caaagaggtc cagcggctgc gggaagagtc tgacggctgg tttttcgata
    53521 tctggcaacc gccgcaggtg gatgaagccg atgctggccc gttgcgccag gcgaacgatg
    53581 gcacggcttt aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat
    53641 tctgacaccg gggatggacg agcagggcaa tatgagcgag gaggggatcc cggcggcgct
    53701 ggtggcaaaa ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cgtataacct
    53761 gctgtttctt tttagtattg gcatcgataa aaccaaggca atgggattat tgcgtgggtt
    53821 gacggaattc aaacgctctt acgatctcaa cctgcggatc aaaaatatgc tacccgatct
    53881 ctatgctgaa gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat
    53941 ccataagctg attcgtaaac acgatctttc cggtttgatg ttgcgggcat ttgatacctt
    54001 gccggagatg atcatgacgc cacatcaggc atggcagaga cagattaaag gcgaagtgga
    54061 aaccattgcg ctggaacaac tggtcggtag agtatcggca aatatgatcc tgccttatcc
    54121 acctggcgta ccgctgctga tgccaggaga aatgctgacc aaagagagcc gcacggtact
    54181 cgattttcta ctgatgcttt gttccgtagg gcaacattac cccggttttg aaacggatat
    54241 tcacggcgcg aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg
    54301 ataacttgcc agagcggctt ccgggcgagt aacgtgctgt taacaaataa aggagacgtt
    54361 atgctgggtt taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc
    54421 aaagcgtttt actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg
    54481 cgcgactcgt ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctca
    54541 tttccgttcc cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct
    54601 tttagcgttg atgatatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt
    54661 gaagccatcc gtgtcgatcc atacacgcaa aaacgcttca cgttctttaa cgatccggac
    54721 gggctgccgt tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac
    54781 gtgccgggca ttgctactgt aaaatcgcat catcatgaca ctcacgctca atagacaact
    54841 tctctcctca cgccagattc tggtggcctt tagcggcggg cttgactcca ccgttctgct
    54901 gcatcagttg gtgcagtggc ggatggaaaa tccgggcgtc actctgcgcg ctatccatgt
    54961 gcatcacggt ttaagtgcta atgccgatgc ctgggttagg cattgcgaga acatctgcca
    55021 acagtggcag gtgccgctgg tggtcgagcg cgtgcaactt gcgcaagaag gactgggcat
    55081 tgaggcccag gcgcggcagg cacgttatca ggcatttgcc cgcaccttgt tacccggtga
    55141 agtgctggtc accgcgcaac atctcgacga tcaatgtgaa acctttctgc tggcgctaaa
    55201 acgcggtagc ggccctgccg ggctttcggc tatgggggaa gtttcggagt ttgccggaac
    55261 acggcttatc cgcccgttgc tcgcccgcac gcggggagaa ctggtgcagt gggcgcgtca
    55321 gtatgattta cgctggattg aagacgaaag taatcaggat gatagctacg atcgtaactt
    55381 cctgcgcctg cgcgtggtgc cgttattgca gcagcgttgg ccgcattttg ccgaagcaac
    55441 ggcccgcagc gctgcgcttt gcgctgaaca agagagcctg ttggatgaac tgctggcgga
    55501 tgatatagca cactgtcaaa cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat
    55561 gagtgatgcc cgccgcgcgg cgattatccg ccgctggctg gcagggaaga atgcaccgat
    55621 gccttcccgc gacgcgttgg tgaggatctg gcaggaagtg gcgctggcgc gggaagatgc
    55681 ttccccctgt ttacgcctgg gcgcgtttga aatccgccgc tatcagtcgc aactgtggtg
    55741 gattaaatcc gtcactgggc aaagcgaaac cattgtgccg tggcagacgt ggctacaacc
    55801 gctggaatta ccggcgggac tgggaagtgt acagcttact gcgggaggcg atattcgccc
    55861 tccgcgtgca gacgaagcgg tcagcgtgcg tttcaaagcg ccaggattgc tgcatattat
    55921 cgggcgcaac ggcggacgta agctgaagaa aatctggcaa gagctgggtg tgccgccgtg
    55981 gctacgtgac accacgccac tgctgtttta tggcgaaacg ttgattgcgg cggcaggggt
    56041 atttgtgacg caagaaggtg tggctgaagg tgagaatggc gtcagttttg tctggcagaa
    56101 aacgcttagt taagtgaaag ccggataaga cgcttcaaac gtcgcatccg gcgaaagtca
    56161 atcaggactc gctcaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat
    56221 ccagacgcag ctcgcgggtt gtaccagcgg cctcgacgac cagatactcc acatttttgc
    56281 gggagactaa atcgctggcc ttcgcctgca atttttcgcc atctttcagc tcaagtgtca
    56341 gcattaaatg atgctggcag gcgagctcaa gattatcgta atcatcacaa ttgattggtt
    56401 gatacgtatc attcattgac ataatcgctc accagtaagt ttgccgcagc gtatgctgct
    56461 ttttccctga cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa
    56521 acgcagccca gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc
    56581 ttgcgtatta actcacaata tttttccaca tgccctcctg tcagcactct gacttaaccg
    56641 tggatgcaag tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact
    56701 catttctgca atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg
    56761 aaaggagttt tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg
    56821 atctcgaccg caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa
    56881 cccaggagcg catgatgctg cgcctgctgg cgtggctaaa atatgccgat gaacgtctgc
    56941 aatttacccg tggtttgtgt gccgatgatg agccggaagc gtggctgcgt aacgatcatt
    57001 tgggtattga tttgtggatt gagctggggc tgccggatga gcgacggatt aagaaagcct
    57061 gcacccaggc cgcagaagtg gcgctgtttg cctataatag tcgggcggcg caaatctggt
    57121 ggcagcaaaa tcagagcaaa tgcgcgcagt tttccaatct ttccatctgg tatctggacg
    57181 atgaacaact ggcgaaagtg agcgcctttg ccgatcgtac catgacgctg caggcaacga
    57241 ttcaggatgg cgtgatctgg ttatcggatg ataagaataa tctggaagtg aacttaaccg
    57301 cctggcaaca accttcatga ttgtgatttc ccgacatgtt gctatccccg atggtgagct
    57361 tgagatcacc gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac
    57421 ggctattcat ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagaacg
    57481 tctgctcgcc gccagccatc acttgatcag cagtgatggc gtgattgtca ttaaggcaca
    57541 ggaataccgc agtcaggaac tgaaccgcga agcggctctg gcccggctgg tggcagtgat
    57601 taaagattta acaacagaac aaaaagcccg acgacccacg cgccccaccc gtgcatcgaa
    57661 agagcgcagg ctggcatcga aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt
    57721 gcgcagcggt cgggaataaa aagaaggaat ggatggtgaa aaaagcgata gtgacagcga
    57781 tggctgtaat cagcctcttt actctgatgg gttgtaataa tcgggccgaa gtcgatacgc
    57841 tttctccggc gcaggctgcc gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc
    57901 cgtgtgctga ttgcgaagga atcgaaacct ctctgttcct cgaaaaagac ggaacatggg
    57961 tgatgaatga gcgttatctc ggtgctcgtg aagaaccttc ctccttcgct tcctacggta
    58021 catgggcgcg aaccgctgac aagctagtat taaccgacag caaaggtgaa aagtcatatt
    58081 atcgggcgaa aggcgatgcg ctggagatgc tcgatcgtga aggcaatccg attgaatcgc
    58141 agttcaacta tacgctagaa ccggcacaat ccagtttacc tatgacgccg atgaccctgc
    58201 ggggcatgta tttttatatg gctgatgcgg cgaccttcac tgattgcgcg accggaaaac
    58261 gtttcatggt agcgaataac gcagagctgg agcgtagcta cctggctgcg cgcggtcaca
    58321 gtgaaaaacc ggtgttattg tcagtagaag gtcactttac gcttgagggt aatccggata
    58381 ccggtgcgcc gactaaagta ttggcacccg atacggcggg taaattttat ccgaaccagg
    58441 attgcagtag tttggggcag taattgaaag ccggagcatt ctccggcttt tatcactgag
    58501 tcagcccaac aaagcgaccc gccttgatat aaatccctgg cttcatgtgc ccaacatact
    58561 gtaactgcgt ttctggctta aacgcagaga tatcgccagt acgcatatgc atcagatcgg
    58621 cagggctaat ccagcctgat tgcgccagcg taagcggatg cccggctttg gcaaatgcat
    58681 cagtgacaaa ttccgaacaa aaccacgact ttttgtctcc ttcgcccaca ctgcttaact
    58741 gcgctttcgc caggccgctg acgcactgtt gacgaaaatc ctcggagaac ggattcagtg
    58801 agcacacctg gcgagtcacc ataaagggaa taaattcgac aatgccgcga tagttataac
    58861 cgctatcttt gattttatta gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat
    58921 ccgggactcg taagacgaaa agcttatcac tgtgcttcat cgctttttta agggaaacga
    58981 tctggacgcc agcgcctgtc gcttctgcaa cgttattatc acccagaaag attgcaacgt
    59041 gactcacaga ggaagtgctg aagacgcgga ttccaaatga ggttacccca aggcttgagg
    59101 agaacagcaa atcgccgggt ttgagatcag gtgccgttat ttcttttatt gattgttcgg
    59161 tgaaagagct ttgatgctgg aatttgacta cccatgtttt tgcctcgaca tctaccgctg
    59221 tggcagagga gtctggctgg ctgatatcaa cggtacaggc cgaaaggaaa agaaagcagg
    59281 ggagaagcag gcggcaatgc gcctttggtt tatccatttt atacaatcca tgtaaaaaag
    59341 ggccctgaaa ttcaggaccc tttctggcat cagcctttaa tctgtttcac cagatattcg
    59401 acgatgtcac cagtcttaat taactgtttc tcgccgttac gacgatattt atattcgata
    59461 tcatcgttgt cgaggttacg gtcgcccagc acaatagtgt gcggaatacc gatcagttcc
    59521 atatcagcaa acatcacgcc cggacgctct ttgcggtcat ccagcagcac ttcgatacct
    59581 tgtgcacgca gttcgctgta cagtttctca gcaagctctt gtacgcggaa ggatttgtgc
    59641 atgttcatcg gcagaatcgc cacctggaaa ggcgcgatag cgtcaggcca tacgatgccg
    59701 cgttcgtcat agttctgctc aattgccgca gccacaacgc gcgttacccc gataccgtag
    59761 caacccatcg tcaggatttg attacggcca tcttcaccct gtacggaggc tttcagtgct
    59821 tcggagtact tggtacccag ctggaagatg tgaccaactt cgataccacg tttgatcagc
    59881 agcgtaccct gaccatccgg gcttggatcg ccagccacca cgttgcggat atcagcaatt
    59941 tccggggtag cgacatcgcg atcccagttg ataccgaagt agtgtttacc atcgatgtta
    60001 gcaccagcag cgaaatcact catcgccgca acggtacggt caatcaccac cggaattggc
    60061 atgtttaccg gacccagtga gccagggccg gctttaacca cggcacgaat ttcttcttcg
    60121 gtcgcgaaag tcagcgggct tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc
    60181 tcgtggtcac cgcgcaccag cagcgcaacc agcgggaagc tactgccttc aaccgcttta
    60241 accagcaggg tcttaaccgt tttctcaatc ggcagattga actgttcaac cagttccgcg
    60301 atggtcttcg cgttcggcgt atcaaccagc gtcatttcct gggtagcagc agcgcgcggt
    60361 tctttcggcg cgatagcttc tgccagttca atgttcgctg catagtcaga ggtgtcggag
    60421 aagaccacat cgtcttcacc gctttgcgcc agcacctgga attcgtgaga ggcgctaccg
    60481 ccgatagaac cggtatcggc ctgtacggcg cggaaatcca gccccatgcg gctgaagatt
    60541 ttgctgtagg ccgcatacat tgcatcgtag gtttcctgca gggattcctg agaggtatgg
    60601 aaagagtaag catctttcat caggaattcg cgggaacgca tgacgccgaa acgcgggcgc
    60661 acttcatcac ggaacttagt ctggatctga tagaagttca gcggcagctg tttgtaagag
    60721 ctcagctcgt tacgaatcag gtcggtaatc acttcttcat gagttgggcc aagtacgaac
    60781 ggacggtcac cacggtcagc aatacgcagc aattccgggc catactgttc ccaacgacca
    60841 ctctcttgcc acagttcaga tggctgaacc accggcatta acacctcaat cgcaccggcg
    60901 ttgttcatct cttcacgcac gatgttttcg acttttttca gaacgcgcac gccggtcggc
    60961 agccaggtgt ataacccgga ggccagcttg cggatcatcc cggcgcgcag catcagctga
    61021 tggctgatca cctcggcgtc ggcaggtgtc tccttgagag tggagagcag gtattggcta
    61081 gtacgcatgt tgttacggtt ccagttggaa ggtagaacag gctcaaggtg agcctgggac
    61141 aaaaaaagtg atttagttta ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt
    61201 aacgcggttc cagcgcaaag acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa
    61261 aatcatgcag ccagacggca taggttttgc ccgtttcctc acctttacga taggccgggc
    61321 gcgggtcctg cgctagtact tcgcggataa acagcgttaa ctgcggataa cgcttctcca
    61381 gcgtcaaaag ctgcttttcg acctctgcgg taaaactcac cgccatctct gcagctggcg
    61441 cactttgcgc atagctggca ctggcatttg gaagcgattc ggcaaagggg agatacggtt
    61501 tgatatccac taccggcgta ccatcgacca gatccagact gccgagcttc agaatcacgc
    61561 tgtctttatg gcaaacaacc tctttcagct ctaccagcga cataccaatt gggttagggc
    61621 gaaaagtgga gcgtgttgcg aaaaccccca ttctggcatt accgccaaga cgaggcggac
    61681 gcacagtcgg acgccagccg ccttccatcg tttgatgaaa gacgaaaagt atccataaat
    61741 ggctgaacgc ttccaggccg cgaacggcgt cggcttggtt gtagggagca atgagatgca
    61801 gttcgccgtt ggcgcttttt accagacctg gttggcgcgg aacggcgaat ttttctttat
    61861 agggcgagcg aataacgcct atttgctcga actggaaact gctcatttcg ccgtaatgtt
    61921 aagcgcagaa ccgatacata cagcctgacg atagcagcct ggcgtaccgc tggtgacttc
    61981 gcagctatgc agtaataccg cattggcttt cattttcgag gcgttgattt gcatccgctt
    62041 acgcgcggtt ggaatgctcg gcggagagtc ctgattagag gcttggcaag agtcgccact
    62101 gacttcaccg agatcgcgga acggtttgcc gactaattct tctgcattgg tataaattcg
    62161 gaccggcgtg gcgcgcggca ctttcggttt tgcagactcc gctttcggct ggggtgcagt
    62221 gctttgaacg ggttcgacag gggatctgct taacatggaa cagccgctta gcatgagtgc
    62281 tactaaacag atcggtaaag cacgcatagt atttcctcaa tgtatgatca aaacgtcaat
    62341 attgaatcag gagcttgtaa aaatgacaag acgggcaagc gcccgtcctg aatgatatta
    62401 caaattgtgg aaacagccta aaaattacca gcctttaaca gcgccgccgt taaacacttt
    62461 gtttgctgct tcgtaaactt cgtcagactg ataagcctgg acgaatttct tcacgttctc
    62521 ggcgtctttg ttatcttcac gcgttacgat caggtttacg tacggggact ctttatcttc
    62581 gacaaagata ccgtctttcg ccggagtcag gccaatctgg ctggcatagg tggtattgat
    62641 aactgccaga gcgatttgcg cgtcgtccag agaacgcggc agctgcggtg cttccagctc
    62701 aacaattttc agatttttgg ggttctcaac aacatcaaga acggtcggca gcaggccaac
    62761 gccatctttc agtttgatca agcccacttt ttgcagcagc agcagtgaac gaccaaggtt
    62821 agttgggtcg tttggcacgg caacctgcga accatcctgc agttcatcca gtgatttgat
    62881 tttcttggag taacctgcaa tcggataaac aaaagtgttg cctactgcga ccagtttgta
    62941 gccacgatct ttcagttgct gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat
    63001 atcgcctttg ctcaatgctt cgtttggcag aacatagtcg ttgaatgtta ccagctcaac
    63061 gtccaggcca tatttgtctt tcgcaacttt ctgcgcgact tctgcaacct gctgttcggc
    63121 accaacaatc acgccgactt taatgtggtt tggatctttt tcatcctgac cgcagcctac
    63181 cagtgccagt gatccaatca gggctcccac tgccgcaaag gttttgaatt tgaacgccat
    63241 accttattcc ttcttcaatt atttatgttg tgttgaacgt tacttgcgag tgacagcccg
    63301 gacgatgcgg tcgcctgcga actgaattaa ataaaccaga atgaccagca ataccagtac
    63361 cgtattcatc accgtcgcgt tgtagccgat gtagccatac tgatagccaa tctgacctaa
    63421 accaccggca ccgactgcac cacccatcgc ggaataaccg accagggtaa tcagggtgat
    63481 agttgccgca tttaccagac ccggcaacgc ttccggtaac agcactttac ggacgatctg
    63541 cattggcgtg gcacccattg cgcgggaagc ttcaattaac ccggttggga tctccagcag
    63601 agcgttctcg accatacggg caataaacgg tgctgcacca acggttaacg gaacaatcgc
    63661 tgcctgcaaa ccaatcgatg taccaacaat aacgcgggta aacggaatca tccataccag
    63721 caagataatg aacgggatgg aacggaaaat gttcacaatc gcagaaacgg tacgatacag
    63781 cttcgcgtta gcaataattt gccccggacg cgtgacataa agcaggacgc caaccggcag
    63841 accaatcaca aatccaaaaa aaccggatac gaaggtcatt gccagcgttt cccatacgcc
    63901 acgaaccagc agccacatca tcggctcaga cataacccag tacctctact tttacatggt
    63961 gttcctgcag ccaggtaatg gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca
    64021 gcatgatgcc gaacttaacg ccaccggcgt aatccatctg cgcgctaata atgttgttgt
    64081 tgacgttgaa acgacgcgcg gtttcagaaa gcagtggggc atcgaccgat tgaccggtaa
    64141 actccagacg caacatcggc acgcagtcag tgaatggctc cgcttgcaga cgttcctggt
    64201 aatcttccgg gatatccaga tgcagggtcg actgaataaa cttctgcgcc agcggcgttt
    64261 tcggatgcga gaacacttca cttaccgtgt cctgctcgat cagttcgcca ttgctgatga
    64321 ccgccacgca atcacaaata cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg
    64381 tcaaacccag acggcggttg atgtctttca gcagttcgag aatagaacgt gtcgttgccg
    64441 gatccagcgc gctggtggcc tcatcacaca gcaatacttt gggattgctg gctaacgcac
    64501 gggcaatcgc cacacgctgt ttctgcccac cggaaagatt cgacgggtag ctatcatgct
    64561 tatcgccaag accaaccaat gacagcaatt ccgtcacgcg acgtttgatc tcatctttcg
    64621 gtgtgttgtc cagctccagc ggcagagcca cgttgccaaa aacagtacgc gaagagagca
    64681 ggttaaaatg ctggaaaatc ataccaatct ggcggcgagc tttggtcaac tcggattctg
    64741 acagcgtggt cagttcctgg ccatcgacca gcacgctacc ctcggttggg cgctccagca
    64801 ggtttacaca acgtataagc gtactcttac ccgcgccaga ggcaccgata acgccataaa
    64861 tttgtccagc tggcacatgc aggctgacgt tgttcaacgc ctggatggtg cgggtgccct
    64921 ggtggaacac tttggtgata ttcgaaagtt ttatcattga ttatttatta tcgtcattaa
    64981 gttagtcgtg gcatttcgaa tgcctggaac gggcaacgcc ttcaatgaaa tggatgttaa
    65041 ggcatccaga cgtctaaatc aatcaggttt atgcgaagag cactttcttg ctggtcgaaa
    65101 catgcgatac tagcgtcaca tgccttatta aggagctata aaaggtggcg aagagcgtac
    65161 ccgcaatttt tcttgaccgt gatggcacca ttaatgtcga tcacggctat gtccatgaga
    65221 tcgacaactt tgaatttatc gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg
    65281 gctttgcgct ggtggtagta accaaccagt ctggcattgc tcgcggtaaa tttactgaag
    65341 cacagtttga aacgctgacc gagtggatgg actggtcgct ggcggaccgc gatgtcgatc
    65401 tggatggtat ctattattgc ccgcatcatc cgcagggtag tgttgaagag tttcgccagg
    65461 tctgcgactg ccgcaaacca catccgggga tgcttttgtc agcacgcgat tatttgcata
    65521 ttgatatggc cgcttcttat atggtgggcg ataaattaga agatatgcag gcagcagctg
    65581 cggcgaacgt gggaacaaaa gtgctggtgc gtacgggtaa acctgttacg cctgaagcag
    65641 aaaacgcggc ggattgggtg ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc
    65701 agcaaaaacc ggcgtaatga ttaaaagatg agcggttgaa ataaaaatgc atttttccgc
    65761 ttgtcttcct gagccgactc cctataatgc gcctccatcg acacggcgga tgtgaatcac
    65821 ttcacacaaa cagccggttc ggttaaagag aaaaatcctg aaattc
//
LOCUS       DAZKJR010000007        65637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKJR010000007 DAZKJR010000000
VERSION     DAZKJR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.007"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..882)
                     /gene="pgrR"
                     /locus_tag="ACHOWS_000456"
     CDS             complement(<1..882)
                     /gene="pgrR"
                     /locus_tag="ACHOWS_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFQ0046097.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLK"
     gene            1020..1940
                     /gene="ycjY"
                     /locus_tag="ACHOWS_000457"
     CDS             1020..1940
                     /gene="ycjY"
                     /locus_tag="ACHOWS_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0046098.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSREYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            2011..2649
                     /locus_tag="ACHOWS_000458"
     CDS             2011..2649
                     /locus_tag="ACHOWS_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0046099.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKAGDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            2940..3668
                     /gene="mpaA"
                     /locus_tag="ACHOWS_000459"
     CDS             2940..3668
                     /gene="mpaA"
                     /locus_tag="ACHOWS_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFQ0046100.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(3643..4608)
                     /gene="ycjG"
                     /locus_tag="ACHOWS_000460"
     CDS             complement(3643..4608)
                     /gene="ycjG"
                     /locus_tag="ACHOWS_000460"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFQ0046101.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQESLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            4727..5233
                     /gene="tpx"
                     /locus_tag="ACHOWS_000461"
     CDS             4727..5233
                     /gene="tpx"
                     /locus_tag="ACHOWS_000461"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFQ0046102.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5277..6818)
                     /gene="tyrR"
                     /locus_tag="ACHOWS_000462"
     CDS             complement(5277..6818)
                     /gene="tyrR"
                     /locus_tag="ACHOWS_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFQ0046103.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(6966..8027)
                     /gene="ycjF"
                     /locus_tag="ACHOWS_000463"
     CDS             complement(6966..8027)
                     /gene="ycjF"
                     /locus_tag="ACHOWS_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFQ0046104.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIEDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8024..9421)
                     /gene="ycjX"
                     /locus_tag="ACHOWS_000464"
     CDS             complement(8024..9421)
                     /gene="ycjX"
                     /locus_tag="ACHOWS_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFQ0046105.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            9576..10574
                     /gene="ycjW"
                     /locus_tag="ACHOWS_000465"
     CDS             9576..10574
                     /gene="ycjW"
                     /locus_tag="ACHOWS_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0046106.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGNQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMSLK"
     gene            complement(10625..11530)
                     /gene="ompG"
                     /locus_tag="ACHOWS_000466"
     CDS             complement(10625..11530)
                     /gene="ompG"
                     /locus_tag="ACHOWS_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFQ0046107.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11575..12657)
                     /locus_tag="ACHOWS_000467"
     CDS             complement(11575..12657)
                     /locus_tag="ACHOWS_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0046108.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFLNYALYPHMTVYDNMAFGLK
                     MQKIANEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTISEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(12671..13330)
                     /gene="pgmB"
                     /locus_tag="ACHOWS_000468"
     CDS             complement(12671..13330)
                     /gene="pgmB"
                     /locus_tag="ACHOWS_000468"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0046109.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     ADLRAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(13327..15594)
                     /gene="ycjT"
                     /locus_tag="ACHOWS_000469"
     CDS             complement(13327..15594)
                     /gene="ycjT"
                     /locus_tag="ACHOWS_000469"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFQ0046110.1"
                     /translation="MIRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWCTSNGAGYTITSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKRVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLGTAK
                     FWISRAVQVNDRLEIHNVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARKFGCSDDA
                     FIHRAEIFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(15591..16646)
                     /gene="ycjS"
                     /locus_tag="ACHOWS_000470"
     CDS             complement(15591..16646)
                     /gene="ycjS"
                     /locus_tag="ACHOWS_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0046111.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(16656..17444)
                     /gene="ycjR"
                     /locus_tag="ACHOWS_000471"
     CDS             complement(16656..17444)
                     /gene="ycjR"
                     /locus_tag="ACHOWS_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0046112.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVITACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEKVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALLEQLRADNFQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(17463..18515)
                     /gene="ycjQ"
                     /locus_tag="ACHOWS_000472"
     CDS             complement(17463..18515)
                     /gene="ycjQ"
                     /locus_tag="ACHOWS_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0046113.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGSDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(18546..19388)
                     /gene="ycjP"
                     /locus_tag="ACHOWS_000473"
     CDS             complement(18546..19388)
                     /gene="ycjP"
                     /locus_tag="ACHOWS_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0046114.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19375..20256)
                     /gene="ycjO"
                     /locus_tag="ACHOWS_000474"
     CDS             complement(19375..20256)
                     /gene="ycjO"
                     /locus_tag="ACHOWS_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0046115.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20277..21569)
                     /gene="ycjN"
                     /locus_tag="ACHOWS_000475"
     CDS             complement(20277..21569)
                     /gene="ycjN"
                     /locus_tag="ACHOWS_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0046116.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITVGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            complement(21583..23262)
                     /gene="ycjM"
                     /locus_tag="ACHOWS_000476"
     CDS             complement(21583..23262)
                     /gene="ycjM"
                     /locus_tag="ACHOWS_000476"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFQ0046117.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSTFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYIRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     IDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLRYLIYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            complement(23476..23790)
                     /gene="pspE"
                     /locus_tag="ACHOWS_000477"
     CDS             complement(23476..23790)
                     /gene="pspE"
                     /locus_tag="ACHOWS_000477"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFQ0046118.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(23865..24086)
                     /gene="pspD"
                     /locus_tag="ACHOWS_000478"
     CDS             complement(23865..24086)
                     /gene="pspD"
                     /locus_tag="ACHOWS_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFQ0046119.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(24095..24454)
                     /gene="pspC"
                     /locus_tag="ACHOWS_000479"
     CDS             complement(24095..24454)
                     /gene="pspC"
                     /locus_tag="ACHOWS_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFQ0046120.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(24454..24678)
                     /gene="pspB"
                     /locus_tag="ACHOWS_000480"
     CDS             complement(24454..24678)
                     /gene="pspB"
                     /locus_tag="ACHOWS_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFQ0046121.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(24732..25400)
                     /gene="pspA"
                     /locus_tag="ACHOWS_000481"
     CDS             complement(24732..25400)
                     /gene="pspA"
                     /locus_tag="ACHOWS_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFQ0046122.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            25567..26544
                     /gene="pspF"
                     /locus_tag="ACHOWS_000482"
     CDS             25567..26544
                     /gene="pspF"
                     /locus_tag="ACHOWS_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFQ0046123.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(26664..27929)
                     /gene="puuE"
                     /locus_tag="ACHOWS_000483"
     CDS             complement(26664..27929)
                     /gene="puuE"
                     /locus_tag="ACHOWS_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFQ0046124.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKMFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDEAMKILQDALSD"
     gene            complement(27967..29247)
                     /gene="puuB"
                     /locus_tag="ACHOWS_000484"
     CDS             complement(27967..29247)
                     /gene="puuB"
                     /locus_tag="ACHOWS_000484"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFQ0046125.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVHELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(29249..30736)
                     /gene="puuC"
                     /locus_tag="ACHOWS_000485"
     CDS             complement(29249..30736)
                     /gene="puuC"
                     /locus_tag="ACHOWS_000485"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFQ0046126.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(30886..31443)
                     /gene="puuR"
                     /locus_tag="ACHOWS_000486"
     CDS             complement(30886..31443)
                     /gene="puuR"
                     /locus_tag="ACHOWS_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFQ0046127.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(31470..32234)
                     /gene="puuD"
                     /locus_tag="ACHOWS_000487"
     CDS             complement(31470..32234)
                     /gene="puuD"
                     /locus_tag="ACHOWS_000487"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFQ0046128.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            32446..33864
                     /gene="puuA"
                     /locus_tag="ACHOWS_000488"
     CDS             32446..33864
                     /gene="puuA"
                     /locus_tag="ACHOWS_000488"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFQ0046129.1"
                     /translation="METNIVEVENFVQQSEERRGSSFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            34013..34177
                     /locus_tag="ACHOWS_000489"
     CDS             34013..34177
                     /locus_tag="ACHOWS_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046130.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            34167..35552
                     /gene="puuP"
                     /locus_tag="ACHOWS_000490"
     CDS             34167..35552
                     /gene="puuP"
                     /locus_tag="ACHOWS_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFQ0046131.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            35686..35931
                     /gene="ymjA"
                     /locus_tag="ACHOWS_000491"
     CDS             35686..35931
                     /gene="ymjA"
                     /locus_tag="ACHOWS_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFQ0046132.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            36244..37887
                     /gene="sapA"
                     /locus_tag="ACHOWS_000492"
     CDS             36244..37887
                     /gene="sapA"
                     /locus_tag="ACHOWS_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFQ0046133.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            37884..38849
                     /gene="sapB"
                     /locus_tag="ACHOWS_000493"
     CDS             37884..38849
                     /gene="sapB"
                     /locus_tag="ACHOWS_000493"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFQ0046134.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            38836..39726
                     /gene="sapC"
                     /locus_tag="ACHOWS_000494"
     CDS             38836..39726
                     /gene="sapC"
                     /locus_tag="ACHOWS_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFQ0046135.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            39726..40718
                     /gene="sapD"
                     /locus_tag="ACHOWS_000495"
     CDS             39726..40718
                     /gene="sapD"
                     /locus_tag="ACHOWS_000495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFQ0046136.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            40720..41526
                     /gene="sapF"
                     /locus_tag="ACHOWS_000496"
     CDS             40720..41526
                     /gene="sapF"
                     /locus_tag="ACHOWS_000496"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFQ0046137.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(41575..42738)
                     /locus_tag="ACHOWS_000497"
     CDS             complement(41575..42738)
                     /locus_tag="ACHOWS_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFQ0046138.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFIRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(42747..44120)
                     /locus_tag="ACHOWS_000498"
     CDS             complement(42747..44120)
                     /locus_tag="ACHOWS_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFQ0046139.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAAQETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQIGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(44124..47231)
                     /locus_tag="ACHOWS_000499"
     CDS             complement(44124..47231)
                     /locus_tag="ACHOWS_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFQ0046140.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(47231..48352)
                     /locus_tag="ACHOWS_000500"
     CDS             complement(47231..48352)
                     /locus_tag="ACHOWS_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFQ0046141.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(48501..49067)
                     /locus_tag="ACHOWS_000501"
     CDS             complement(48501..49067)
                     /locus_tag="ACHOWS_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0046142.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            49545..50333
                     /gene="fabI"
                     /locus_tag="ACHOWS_000502"
     CDS             49545..50333
                     /gene="fabI"
                     /locus_tag="ACHOWS_000502"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0046143.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            50477..51604
                     /gene="yciW"
                     /locus_tag="ACHOWS_000503"
     CDS             50477..51604
                     /gene="yciW"
                     /locus_tag="ACHOWS_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFQ0046144.1"
                     /translation="MEKRHITGKSYWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARSIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMNSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            51672..53606
                     /gene="rnb"
                     /locus_tag="ACHOWS_000504"
     CDS             51672..53606
                     /gene="rnb"
                     /locus_tag="ACHOWS_000504"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFQ0046145.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(53683..53787)
                     /locus_tag="ACHOWS_000505"
     CDS             complement(53683..53787)
                     /locus_tag="ACHOWS_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046146.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            53841..55826
                     /gene="pdeR"
                     /locus_tag="ACHOWS_000506"
     CDS             53841..55826
                     /gene="pdeR"
                     /locus_tag="ACHOWS_000506"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFQ0046147.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(55808..55936)
                     /gene="ymiD"
                     /locus_tag="ACHOWS_000507"
     CDS             complement(55808..55936)
                     /gene="ymiD"
                     /locus_tag="ACHOWS_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFQ0046148.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIITEYIAKYIYSSTYARFR"
     gene            55974..56147
                     /gene="yciZ"
                     /locus_tag="ACHOWS_000508"
     CDS             55974..56147
                     /gene="yciZ"
                     /locus_tag="ACHOWS_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFQ0046149.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            56237..56986
                     /gene="yciT"
                     /locus_tag="ACHOWS_000509"
     CDS             56237..56986
                     /gene="yciT"
                     /locus_tag="ACHOWS_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFQ0046150.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEIILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            57255..57473
                     /gene="osmB"
                     /locus_tag="ACHOWS_000510"
     CDS             57255..57473
                     /gene="osmB"
                     /locus_tag="ACHOWS_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFQ0046151.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(57599..57925)
                     /gene="yciH"
                     /locus_tag="ACHOWS_000511"
     CDS             complement(57599..57925)
                     /gene="yciH"
                     /locus_tag="ACHOWS_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFQ0046152.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(57925..58662)
                     /gene="pyrF"
                     /locus_tag="ACHOWS_000512"
     CDS             complement(57925..58662)
                     /gene="pyrF"
                     /locus_tag="ACHOWS_000512"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0046153.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(58853..60022)
                     /gene="lapB"
                     /locus_tag="ACHOWS_000513"
     CDS             complement(58853..60022)
                     /gene="lapB"
                     /locus_tag="ACHOWS_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFQ0046154.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(60029..60337)
                     /gene="lapA"
                     /locus_tag="ACHOWS_000514"
     CDS             complement(60029..60337)
                     /gene="lapA"
                     /locus_tag="ACHOWS_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFQ0046155.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            complement(60486..61250)
                     /gene="pgpB"
                     /locus_tag="ACHOWS_000515"
     CDS             complement(60486..61250)
                     /gene="pgpB"
                     /locus_tag="ACHOWS_000515"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFQ0046156.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            61420..62010
                     /gene="ribA"
                     /locus_tag="ACHOWS_000516"
     CDS             61420..62010
                     /gene="ribA"
                     /locus_tag="ACHOWS_000516"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFQ0046157.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(62074..64749)
                     /gene="acnA"
                     /locus_tag="ACHOWS_000517"
     CDS             complement(62074..64749)
                     /gene="acnA"
                     /locus_tag="ACHOWS_000517"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFQ0046158.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGNITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDDVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(64899..64994)
                     /gene="ymiC"
                     /locus_tag="ACHOWS_000518"
     CDS             complement(64899..64994)
                     /gene="ymiC"
                     /locus_tag="ACHOWS_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFQ0046159.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(65108..65275)
                     /gene="yciX"
                     /locus_tag="ACHOWS_000519"
     CDS             complement(65108..65275)
                     /gene="yciX"
                     /locus_tag="ACHOWS_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFQ0046160.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQQKQ"
     gene            complement(65278..65418)
                     /gene="ymiA"
                     /locus_tag="ACHOWS_000520"
     CDS             complement(65278..65418)
                     /gene="ymiA"
                     /locus_tag="ACHOWS_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFQ0046161.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        15895 a        17457 c        16137 g        16148 t
ORIGIN      
        1 cttcagccta tctataaata atgagaatgc cgagccagca tgtcgacggt gtggatagta
       61 caggtgataa ccgggtaaat ctggtgtgaa tttatccaaa acacgtatca gttttttttc
      121 tttaatagcc cgctcaacct gatcataagg tagatacgcc aatccatgcc catcaattgc
      181 agcatcaatg atcaggtcta tcgtattcag taaaagctga ccttccatgc gaacacgaac
      241 ttcacgtcca ccgcgtatta atctccagcg atttgctgta cccgatgtgg gaagatacaa
      301 attaattgcc tgatgatcta ttaattgtga cactgaggtt ggagcacttc gacgagaaaa
      361 ataatccggt gagccaacaa tagccattgg tatatctggc ccgattcgaa tggcgatcat
      421 atctttatcc atctccccac ccagacggac gcctgcatca aaacgttcag aaacgacatc
      481 ggtcaaacca taatcaatgg taagctggat atcaatttca ggatgcgatt tcaggaatgt
      541 gcgcatagct ggtaacaata tcgtttttgc tgcatgttct acagtagtaa tacgtattgt
      601 cccggatggg cggttctgca gatcgctcag ggatgtcagg gcagaatcta tgtcatgcaa
      661 catagggcca agaacagaca aaagatgctc gcccgcttca gtgggtacaa cgctgcgcgt
      721 ggttcgtgtc agaagccgca atcccaatcg ttcttctata cggcgcacta tctggcttaa
      781 agctgactgc gccatgctca ggcgggctgc tgcgcgagtg aagctacgct cctctgcaac
      841 gacgacaaac gccatcagat cagcgatttc ttcacgcttc attcagtatc tccatacaat
      901 ctattaatca tttaaatgat aggtctaatc atgattttgg gtctaatcaa cttaatgatc
      961 aatatctata gtgatatcac caaccgtcaa aacgaaccga caactccatg aggaacggaa
     1021 tgatgaataa taaagtcagc ttcactaaca gcaataatcc aaccatctct ttgtctgcag
     1081 tgatctattt ccctccaaaa tttgatgaaa cccgtcagta tccggcaatt gtggtatctc
     1141 acccaggtgg gggcgttaaa gagcaaacgg ctggaaccta tgccgaaaaa ctggcggaaa
     1201 agggatttgt cactattgct tatgacgcat cttatcaagg tgaaagcggc ggcgagccgc
     1261 gtcagttaga aaacccatac attcgtactg aagacgtcag tgccgtgatt gattacctga
     1321 caacgctttc ttatgtcgac aatagccgaa ttggtgcgat gggaatttgt gccggtgcag
     1381 gatatacagc caatgctgca attcaggatc gccgcatcaa ggccatcggt actgttagtg
     1441 cggtcaatat tggttcgatg ttccgtaatg gttgggaaaa caatgtgaaa tctatagatg
     1501 ccctaccata cgttgacgcg ggttcaaacg cccgaaccag cgatattagc agcagagaat
     1561 acgccactat gccactggca ccaatgaagg agtccgatgc gccgaacgaa gaactgcgtc
     1621 aggcctggga gtattaccat acccctcgcg cgcagtatcc aacagcaccg ggttacgcta
     1681 ctttgcgcag ccttaaccag attattacct atgatgctta ccatatggcg gaagtgtacc
     1741 tgactcagcc gatacagatt gtggcgggca gccaggcagg gagcaaatgg atgagtgacg
     1801 acctgtacga tcgagcatca agccaggata aacgctatca cattgttgaa ggcgcaaacc
     1861 atatggatct ttatgacggc aaagtctatg tggctgaagc aatttcagta ttagctccgt
     1921 tctttgagga aactctgtaa tgaaacatat tcaaatccgt aatagtgata tggcctggca
     1981 tattgctgcc aataatctgg gaaaagtttc atgaaaaatg tactgattct tggtgctggc
     2041 ggtcagatag cccgccatgt gattaatcaa ctggcagata aacaaaccat taaacaaact
     2101 ttatttgccc gtcagccagc aaaaatccac aaaccctatc ccactaatag caagattatt
     2161 atgggggatg tactgaatca tgcagcactg aagcaggcca tgcagggaca ggatgttgtg
     2221 tatgccaacc ttacggggga agatttagat attcaggcaa attcagtgat cgctgcgatg
     2281 aaagccggtg atgttaaacg tctgatcttt gttctttccc tcggtattta tgatgaagtt
     2341 ccaggaaaat ttggcgaatg gaataatgca gttattggtg aacccctaaa accattccgc
     2401 agggctgcag atgcaattga ggcttctgga ctggaatata caattcttcg ccctgcctgg
     2461 ctgactgatg aagatattat tgattatgag cttacctcac gcaatgaacc attcaaaggt
     2521 acgatcgtat cacgtaagag tgtcgcagca ttaattacag atatcatcga taaacctgag
     2581 aaacacattg gtgagaatat aggaatcaat caacccggga ctgatggcga taagccattc
     2641 ttcatgtaaa tcaattatat agctaccatc gtactgtact atgtgcagta cgatttatta
     2701 aacttattaa ggtgaactga tcaatcagat gcgattaaca tgtgtagaac cagtaagaat
     2761 aaaatacgcc atgtatataa cattacgaca attctaatat ctgcacatca atgaaaatgc
     2821 aaacaacttc cggcaaaaaa gtgatacaag atcgataaca tagcgttgaa gaaaaggatt
     2881 tgataatccg caaaatttgc acaacactgc ctgtaatact tcctgaacca gagacgatca
     2941 tgaccgtaac tcgcccacgc gccgaacgcg gcgcatttcc gcccggaaca gaacattacg
     3001 gacgttcatt attgggcgcg ccgttaatct ggtttccggc ccctgcagcc agtcacgaaa
     3061 gtggtttgat tctggctggc actcacggtg atgaaaactc ttcgatcgtc acgctctcct
     3121 gtgctctgcg gacgttgaca ccttctttgc gccgtcatca tgtggtgctg tgtgtgaatc
     3181 ctgacggctg ccagttgggg ttaagggcca atgctaatgg tgtggattta aacagaaact
     3241 ttccggcggc aaactggaag gaaggtgaaa ccgtttatcg ctggaacagc gccgctgaag
     3301 aacgtgatgt cgttttactg acaggtgaca aacctggctc cgaacctgaa acccaggcgc
     3361 tgtgccagct tatacaccgc attcaacccg cctgggtggt ctcattccac gatccgctgg
     3421 cctgtattga agatcccaga cacagcgaat taggtgaatg gctggcccag gcgtttgaat
     3481 tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc tttcggcagt tggtgtgcgg
     3541 atctcaacct gcattgcatc accgcagagt ttccacccat ctcctccgac gaagccagcg
     3601 aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca tcctaaagat gcaattcgcc
     3661 cgttgtgaac tgaagcgccg gttccacatc taccgccagc caggtcggtc cgtcaagatc
     3721 ggcgaaactg acctgcggca ccagcggtaa agcggcgcta atggcacgag aggtacacaa
     3781 catgcagccc agcatcagac taaaaccttg tgcacgtgct tcagtcgcca gcgccagcgc
     3841 ttccgtcagg ccccccgttt tatcgagctt aatgttaacc atctcatagc gccctttcag
     3901 cgccttcaga ttgctacgag tatgacaact ttcatcagca caaatcggta acggatgaat
     3961 aaaattctcc aacgccgcat cgtcctgcgc aggaagcggt tgttcaagca ttgcaacgcc
     4021 taaatccgcc aatagctggc aacgcgccgc caacccttct gcacgccagg attcatttgc
     4081 atcaacgatc agcgtcgcat cgggcacagc ggtgcgaatt gccaccatcc gctcactgat
     4141 aagatggtta tccagcttca ctttcagtaa tttcgcgcct gcctgccaga gtgttgatgc
     4201 actattggcc atctgatcag gcgtaccgat gacaaccgtc tgtgcagtga taactgtccc
     4261 gggaagcgtt atgccgatca aatcagccag cgattcctgc tgttttcgcg ccgcgagatc
     4321 ccacaatgca caatccagcg cattacgtgc tgcgccggca gggaggattt tttgcaactc
     4381 ctcccgtgtc agccctttct ctaattgcgg cacgacgctc ataatttgcg ccattaccga
     4441 ggcatcactt tctccataac gcggatacgg cgtgcattca ccggtgcctt taataccttc
     4501 ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga cttccccgag caatcacaaa
     4561 cggggtatgt aagggccagc cttcctcgaa taccttaacg gttctcatct ttactccttg
     4621 ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg ctgtgttatt gacagttagc
     4681 ataaactagg tgtgacgtta actatatgta aacaggaaag ataatcatgt cacaaaccgt
     4741 tcatttccag ggcaacccgg ttacagtcgc caattccatc ccgcaggcgg gtagcaaagc
     4801 gcagactttt actctcgtgg caaaagatct gtctgacgtc accctcggtc agtttgcggg
     4861 taaacgcaaa gtgctgaaca ttttcccgag tattgatacc ggtgtttgcg ccgcatcagt
     4921 acgtaagttt aaccaactgg caacagagat cgacaacacc gttgtgctgt gtatctctgc
     4981 cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa ggtctgaaca acgttatcac
     5041 cctctccact ttccgtaacg ctgaatttct gcaagcctac ggtgtggcaa ttgctgatgg
     5101 cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt gatgaaaatg acaatgtgat
     5161 tttcagccag ctggtggatg aaatcaccac cgagccggat tacgaagcag ctctggctgt
     5221 actgaaagca taagttaata tgcctgatgg tgttgcacca tcaggcatat tcgcgcttac
     5281 tcttcgttct tcttctgact cagaccgtat tcccgcaact tattggcaat cgcggtatgt
     5341 gaaacgccga gacgttttgc cagtttgcgc gtgctgggat aattgcgata aagctgggtt
     5401 aataccgagc gttcaaaacg actggtgatt tcgtccagcg aaccttccat cgcatcttcg
     5461 cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa tatcctgtgg acgcagctca
     5521 taaccgtcca gttgtgtcag tgcgcgatag atagcgttct ttaactgccg cacatttccc
     5581 ggccacgcat aacgtgtaag tacagtattc aggtcagcgg ccagtttcgg acgcggcacg
     5641 ccctgctcgt cggcaaagcg ggcgacgaac agctcggtta acggcatgat gtcctgcgga
     5701 cagtcacgta gcggcggcag attgagcgtc aacacgttca gacgataata gagatcttca
     5761 cggaacacgc ctttttgcac cagttcgacc aaattcttct gcgtagcgca aatcaccctt
     5821 acatcgacat gcacttcatg atcttcacca actcgacgga aagtaccatc attgaggaaa
     5881 cgcagtaatt tcgcctgcat ccttggcgac atctcgccta tctcatctaa cagcaccgaa
     5941 ccaccgttcg cctgctcaaa gaaccctttt ttaccttccg gagcatgacc aaacagttca
     6001 ctctcgaccg catcttccgg tatagacgcg cagttcagcg ccaggtaagg tttgcccgct
     6061 cgtgggcttg cctgatggca ggcgtaggca aagagatctt tacctgtacc ggtgtcaccc
     6121 gtaatcagca acggcgcgct tagcatcgcc agtttctgcg cctgttcgac aacatgcttc
     6181 attttcgggc tgacggcgac aatttgactg aaggcactga cgtcctgtgc agcgacattc
     6241 tgcaactgac ggcccatacg aatcgtcgag cgcaacatca ccaccgcacc ggtcagaaca
     6301 tgttgatcat tttcatcctg aagataaaca ggcgtaatct ccatcaggaa attctgccca
     6361 ttaataacaa catgctcgtt atgcgagtct tgcggttcgc tttccagcca gcgtaaaaaa
     6421 ttaaagccgt taattaattg cgcggcggta tggttgcgca ggcgatccaa tttttgccca
     6481 aaaagctgac agctcgccgg gttcgccata tccactttgc ttttcatatc aacagaaagc
     6541 acaggctcag gcaacgcctc cagtaacgcg ctcaacgcca gatgctcacg ttcggaaggc
     6601 atccacggaa cagtgcgcac atcggtaaca cctgcaatac ggcgtatttc ggccatcaga
     6661 ctgctgaaac tttcaaattc cagttcggca aaattgaggt agattcgccc aatggggtca
     6721 atctcaatac cgcgtaaatc aatgcctctc agcaccagta gatcgagtaa ttcgcgggtc
     6781 agaccaagtc ggtcttcaca aaagacttcc agacgcatgg gaaccttcac ctgaaaaaag
     6841 aacaattaat atgatgatat gacactattt ggatagcagg aaggtttctg tcaacaatga
     6901 ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga tatccggtca gcacccgata
     6961 ttgcattatt tttcgctggg cgttttgccc ttttgcagcg tctctttcac ctgaccgata
     7021 agctgacgac ggaaatcccc gaggcgaggt ttgtcatctt caatccacgg cagcgggcgg
     7081 caaagttcca tagctttaat ccccagtcgt gccgtcagaa gtccagcacc aatcccctga
     7141 gctgcccggg tagacaaacg cgcagcgaga tcttgcgaca tccagtccat ccccacttcg
     7201 cgcaccagtt cgctggctcc ggcgaaagcg atattcagca ataccagctt aaacaggcgc
     7261 aaacggctgt aatatcccag ttcaatgcca tacagcgtgg cgatgcgatt gatcaaacgc
     7321 agattgcgcc aggcgataaa agccatatcc accagcgcca gtgggctgac cgcaatcatc
     7381 aatgttgatt ccgctgccga acggctgatt tcgcgccgcg cctgggcatc taaaactggc
     7441 tggaccaaat gcgcatacaa actgaccact tcacggtcgt tttgcgtttc atggattgag
     7501 gcataccagc gttgcagcgc cggatgtgac tgatcgatgc ccgcctgctg tgccagtttt
     7561 tcgcaaaatg cgcggccttt acccgtgccg tgactatgca ataaatcacg cgcttcgtcg
     7621 cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc actctgttac cacagaacct
     7681 acgccagcgc cgataatcaa tgccccagcg gcacatccac ccagcgccac ccagtcctgg
     7741 gtttgccagg cgttcattgt ccactgtaca ccctgcccga caacgcttgc gccaaacaga
     7801 gccaacccgc ccatcaccat tttgcgccac aggctgcgtt tcggacgtaa cgctgcgtcc
     7861 atcaccgctt caacttgccc ctcttcttcc tgcgcttcgt cgagcgtggc cggggcaaaa
     7921 ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga attttggatt ctgatcgacc
     7981 tccagtggac cgtcgaaatc aatacgtggt tttaacggtt cggtcatcgc aatttatctc
     8041 ctattaaaaa ttccagcgca gcatcaaggc gaatatgcgg tagcggtttg tcgacatcca
     8101 tcacctgcgg acgaaacgcc tcaaactgga agccttgctt atcccagaac gcctgaccag
     8161 gcaaacgtgc ggggacttcg ccaggataaa cagtgagcgg tgcgccatca ctaagtcgat
     8221 taccgcgcag cgccgggatt ttctcaccgt taacatcaat aatgccgctg gtggtcgcct
     8281 gaactgacgc cagccccagg cagtccatgc tgatcccttc gaacgctgca ttttgccagg
     8341 catcctgaat cagttgttgc agtaatgaaa ccatattagc gtgctgatcg atggtcacat
     8401 ggtccgcttt agtggcagca aacaatagct tatcgataac cggcgaaaac aaacgccgga
     8461 acagggtacg ctgcccgtag tggaaacttt gcatcagctg cgtcagcgcc agacgcatat
     8521 cattaaatgc ctgtggtcca ctgttgagcg gttgcaggca gtccaccagc acaatctggc
     8581 ggtcaaaacg caggaagtga tttttataga atcctttcac caccttctcg cagtaataat
     8641 taaaacgctc gcgcaacatt ccggcgttgg tgtgtttatc ggcctgcgcc agtttagact
     8701 cgccccaggc atcgacatct ggccatggga agaattgcag cgcgggcgca cctgccatat
     8761 ctcccggcaa gacaaagcgc ccaggctgaa taaagtgcag cccctgctgt ttacagtggt
     8821 ggagataatc ggtccacgcg gcggcaatgt ctgccagccg gttttcgtcg gcaggcgcta
     8881 gcgggtccag cccttcgcac atcattcgcc atttcgccga ccattcgccg cgctgaccat
     8941 tgagtaagcc cgtcatctgg cgcgaccagc ttaaatagtc ctgcgccagc atcggcaggt
     9001 cgagcaacca ttcgccgggg taatccacaa tctccaaata cagcgtggag gtatccttaa
     9061 agtggcgcag cagcgaatcg ttcgatttat agcgtagcgc caggcgaatt tcgctgacgc
     9121 cgcgcgttgg cgtcggccag gcgggaggat cgccatataa ctgcgccagt ccttcatcat
     9181 aggtgaagcg cggaatgcca aagtcacgct ggggaatgcg ttttacgccc agcaggcgtt
     9241 cttcacgcac cgcacttaat agcggcaaac gtgctccggc atgaatattg agcaactgat
     9301 taaccatcgc ggtgataaac gctgttttgc cgctgcggct aagtccggtt acagcgaggc
     9361 gcagatgtct gtcgacaccc cgattcacca gcgcattaag ttcattttta agtcgcttca
     9421 ttgctgtcct gtgctgctct gaagaccaga aaaaaatcat tcgtaaggat atgaatgtgg
     9481 gggccaggcc attgattatc aactgcccgt gttgtcatcg acgggcgaac tccataagat
     9541 gtgcaacatt cacgttcgga cggagttcct gacctatgtc ccctactatt tatgatattg
     9601 ccagggttgc aggcgtatca aaatccaccg tatcacgcgt gctgaataag caaaccaata
     9661 tctccccgga agcgcgcgaa aaagtgttac gggccattga agaattacag tatcaaccaa
     9721 acaagctggc acgcgcgctg acctcttcgg gttttgatgc cattatggtg atttctaccc
     9781 gttcgaccaa aactacggcg ggtaatccgt ttttctcgga agttttacat gccatcaccg
     9841 ccaaagctga agaagaaggt ttcgacgtga tattgcagac gtcgcacaac ctggcagaag
     9901 acttacaaaa atgcgaaagc aaaattaagc agaaaatgat taaaggcatt attatgctaa
     9961 gttcgccagc ggatgagtca ttttttgccc aactcgataa atatgatatt ccggtggtgg
    10021 taattggcaa agttgaaggt caatatgccc atgtttattc tgtcgatacc gataattttg
    10081 gcgacagcat tgcgttgacc gatgcgttaa ttgaaagtgg gaatcaaaat attgcctgcc
    10141 tgcatgctcc gcttgatgtc catgtctcgg ttgaccgcgt taatggctat aagcaaagtc
    10201 tggctgcgca taatattgca gtgcgtgatg aatggattgt tgacggcggt tatacccatg
    10261 aaacagcctt gcaagccgca cggcaattat taagccagtc gccgttgcca gaagccgtat
    10321 ttgccactga cagcctgaaa ttaatgagca tttatcgtgc ggcagcagag aaaaatattg
    10381 ctattccgca gcagttagcg gtggtgggct atagcaatga aacgctgtca tttattttaa
    10441 cgcctgcacc gggcggcatc gatgttccga cgcaggagtt agggcaacaa agctgcgagt
    10501 tattattccg cttaattgcc ggaaaaccgt caccacaaaa tattaccgtt gccacgcata
    10561 tgtcgttgaa ataaatgtat gccggatttg gcgctcgcgc cgcatccggc aatttaaccc
    10621 attatcagaa cgagtaattt acgccgacac ctgcataatg gaatttatcg ctgtcgcctt
    10681 cgtcgtgatc ctgccactca aacgcgtatt ccagcgaaac ggaaagacca ttctggaaat
    10741 cataaccgta aaataacccc acacggttaa aatcatggcc ttcacgttca atatcatcct
    10801 gccagtccca gttactccag cgatccagcc caatgcgcgt atacggcgtc accgagtggt
    10861 tgccgagcgt cagcggcaaa taggcgcgaa tttcttgcgt ggaaaactca ccgttattgc
    10921 ggctatcatc catattgaaa ccgcgctcga gataatagtt cactcgcaag gcaaccgttt
    10981 cgttgaaggt atattgcaga cccgtttccg tttcgacacg ggtatcagca taaccggtcg
    11041 tattcagatc gttggcaaat ttatacatcg acagccaacc gttgaaacgt aaatcgtcag
    11101 tcagtttcac atcccagtct ggcgcaattt tccagcgctg catattcgcc gtgtctttac
    11161 ccggttcatc aacgtagtga taaccataat tacggaaacc accagtcaga ccgaaactga
    11221 aatcatcgct ttcgaggaac tgataatgta cctccagctc cgggcgatca aaccacgttc
    11281 cacgtttacc cgcgctataa tctaccggcc cttcctgata ataggccaga gaaattctcc
    11341 acggcccgtt ggcggcatta aaatagacag aaggctccgc cagcccatcc atatcttcgc
    11401 cataaccctc gacgttttct atttcgtaca tcgcgccgat attaaagtgc cagtcgttct
    11461 tttcctcggc ctgtgcgcag gccattcccg cacacatcac cagtgcggta cagggtaata
    11521 attttttcat tattgttcct tgtattatcc tgggggaatg ccttccccct gtatttagcg
    11581 aattgctatt tccgtttctg catcaaagaa atgacattta gtcatatcaa aatgaatagt
    11641 gatattttct cctgcatgat aatcatttaa cgcaccagca cggaccacca gttcgtgccc
    11701 cccaaccgtg gtgtagagca taaattcagc accggttaat tctgccacgc taattttggc
    11761 ggaaatgtta ttttcctctt gcgcgtccgg atgaatatct tccgggcgta ttcccatcac
    11821 aatgggctta tgcaaacttt cctgtgtttt cagaaccgct aatttctcct cggaaatggt
    11881 taatttaagc gtttccgtaa cgaatttatc gccatcaatc gtgccgcgaa taaaattcat
    11941 cgccggtgat cctataaatc cggcaacaaa catattcgcc ggttggttat aaaccgtttt
    12001 cggcgcacct acttgctgaa caatcccatc cttcataatc acaatccgcg tcgccatggt
    12061 catcgcttcg gtctgatcgt gggtcacgta gatcatggtg gtgttcagtt tctgatgcag
    12121 cttgctgatc tctgcgcgca tttgcacgcg cagcttggca tccaggttag agagcggttc
    12181 atccattaaa aacacgcccg cttcgcgcac aatcgcccgc ccgagcgcca ctcgctgacg
    12241 ttgcccgccg gaaagcgccc ccggcttgcg tttcaggtac tcacgcaggc cgagaatttg
    12301 cgccgcccag ttcacccgct catcaatcac ctcgttggcg attttttgca tcttcaggcc
    12361 aaacgccatg ttgtcgtaga ccgtcatatg cggatacagc gcgtagttca ggaacaccat
    12421 cgcgatattg cgctctttag ccggaacgtc attcattcgt ttgccgtcga tcaacagatc
    12481 gccgccgctg atctcctcaa gcccggcaat catgcgcagc gtagtcgatt taccgcagcc
    12541 tgacggaccg acaaagacaa tgaactcttt atcggcaatt tccaggttga agtccttcac
    12601 cacatgcacc tggttatcgt agattttttg aatatgttgt aacgaaagct gagccatgtt
    12661 gattcctttg ctatacgttt tgccagaagg ccgataaccg cggccaggtc agggagtccg
    12721 ttgagggcaa caataattgc gccccggtta agcccgcgcc gatccccacc gagcgcatac
    12781 cgctggcgtt aatggcgtca atgcccgcct gcgcatcttc aatgccgata catgcctgcg
    12841 gcggcacgcc cagtccggca caggcggcga gaaagatttc cgggtccggt ttcgagtttt
    12901 taagttggga agcgtccgcg cagaaggtga aaaactcgcg cagctccagc gctgctaaaa
    12961 tcgtcggcgc attcagggag acagaagcca gccctatagg gatctgctgt gcacggagat
    13021 ctgccagcaa attgcgaatg ccgggcagca cagcattaac cgtcagttcg cgcagtgagt
    13081 ggacgtagag cagatttttg cgatacgcca gttgcgccct ctcctgcggg ttaaagtcgc
    13141 cctctttgcc cccgtgttgc agaatgcgcc gcagagactc atcgcggctg atccctttta
    13201 gggattcgtt aaactgcgca tcaatgctga tgccaatttc agcggcaatc tgctgccacg
    13261 ccttgaaatg cagatgcgcg gtatcggtga ttacaccatc cagatcgaaa attacccctt
    13321 gcagtttcat tcatcctcct gatgcgtggt agcggtccca ttgaagggca aaataaaatc
    13381 acccaaacag aaaacagatt cttcagccac agatataagc tgcccgttca aacgcagtga
    13441 aacgggcgca gaagttcgaa tcgcaatacg ctgcgcgtcg agagtgacct gtaattcgca
    13501 gccctgccag aacagaggga aagacaactg ttgccactgc tcaggtaacg ctggattgag
    13561 atgcaattca ccgttacgca cgctcacccc ggcaaaaccc tgaatcgccc ccagccaaat
    13621 agcgccagtt gcggctgcgt ggataccgtc atcacagcta tgcggatcag caccaagatc
    13681 gatttcagtc ccctcgcgcc agaactgata actttgcgcc aacaggccac agcgtgcggc
    13741 aacaatgccg tggatcgctt tactcagtga agagtcgtga atggtgcgcg gttcataaaa
    13801 ttgcagattg gcaagacacg atgccgctga gtactgctcc ggcagcatgt aattgagcat
    13861 caccacatca gcttgtttga ggatctgcat ctcgttcact tctgcgcgtg aataatccag
    13921 cagaatggtt tgcttccccg ccgccgcttt gtatttcgcc agattaatcg ctggtttcgc
    13981 cataaacgaa tcatcctgcg gcaaaacgcc gtcaggctga atttctggca gccgcagttc
    14041 tttgaggaat atttcggcac gatgaataaa cgcgtcgtcg ctacaaccaa acttacgagc
    14101 gatattcagc gcctgctgga cgttgtaata cgccatatag ctggtgaagg cgttattatt
    14161 gacatgttcg gtatattcgt ctggcccaat aacattgtga atttccagac gatcgtttac
    14221 ctgtacagcg cggctaatcc agaattttgc ggttcccaga agtaacgcca tgccttcgtg
    14281 ggcaatgaaa ctttcatccc ccgttgcctg ccagtattga ataaccgccc aggcgatatc
    14341 agccaccaaa tggtgttcag cctgcgccga ggccactttc tggcgcagtc cggtgcggat
    14401 gttaatggcg gcaaattccg gcgtctcttc ttcgccgctg cgcgcgcttt cccacggaaa
    14461 cagagcgccc tgccagccgt tgcgtcgcgc tttctcctgc gcgccaggca agttgtgcca
    14521 gcgataacgc agtaaacttc gggcaaccgt cggatcgcta aacagatgga acggtagcaa
    14581 aaatacttct gtatcccaga aaacgtggcc tttgtagcct tcgccggtta aacctttcgc
    14641 cgcaatgctg ctgcgctcat cgtgggcagg cgtcatgatg cgcaggtgat aaagcgcata
    14701 atctaacgct tgctgatcgt gcgcatcgcc gccgtttacc gtgatacgac gtttctgcca
    14761 ccattgacgc cagttttctg ttgatgctgc aagaagttgg tcgtaactct gctgcgcgca
    14821 catttcaagc tgacgaagcg acgcactgcc ccattcgtct aaagcggctt gtctgtcgtc
    14881 ccgccagtcg atccacaccc gtttttgcag cgtcagcgtc tcacctgcgt gaagctgcgc
    14941 gcaggtatgt tgcagtaaac ggcgctcttt ggcggtatag cattgctgca catcaccgct
    15001 caccttacag caacagctga tggccacatc actgcgccca tcctgggtgg tgtagatccc
    15061 ctgcatcaga tgctgaccaa atacccgcac ctgggtttcg tcgagatgtt ggcgaccgtg
    15121 gttggtttgc gtggcgtcaa tgcctgttga aatcagcact aacgcgtcgg cgtccagtgg
    15181 cgtaatagtg atttccagcg caatgagcgg cagttggtct gccgaaacaa agcgacggct
    15241 ggtgatggtg taacctgcgc cgttgctggt acaccagaca acgttgcggc gtaattcgcc
    15301 actggcaaag ttaagctcac gctgccatgc ttcgcgggat aacgagaaaa tctcaccatt
    15361 tatggcaatc tccatcccca cgacatcagg caggttcacc agttcgttga tttcaccttt
    15421 acccgcccga tgataaagcc ctgccagata catgccgcgc gtctggcgcg tgtaatcttc
    15481 ttcatggctg gcgcgaagcc caagatagcc gttcccctgc gccatcagcg aagcatactt
    15541 gttcagggta tgctggctga aatggggttc cgttaacgtt actggcctga tcataattcc
    15601 acacgcgtcc ctgtttctgc ggattgataa agcgccgcca ccagttgctg gatgatgtat
    15661 ccctgctcgg cgtcggctat catcacgggc ttaccttgta cgtggttgat aaaggcttcc
    15721 atactgcgca gatgacggtt gtcgtctgcc atttcccgtt gcatcagcgt cattaactca
    15781 ccgttgttat cggtgtagat atgtgctgga aacagcgtcg ccccggcttt atcaccacaa
    15841 aagctgacgt tcataatcga ctgttcgcgg atgttgagtg caaatgaggt ctcaagccac
    15901 agaatgccgc cgttatgaaa ttcaatggtg ccaaacagcg aatcttcgac gctgtaggtt
    15961 gccgggtccc actcgccaaa ttgaccgcag ctcttttgcg tgccgatctt ttgaaagcta
    16021 tgcgcagtca cgcttttcac cgctggaaaa cccagcacat acatcgccgc atccagcata
    16081 tgaatgccga tgtcgatcag cgggcctccg ccctgcaatt ctttattagt aaagacaccc
    16141 cagccgggaa caccgcaacg acgcagggcg cgggcggtgg taacgtaaat ctctcccaaa
    16201 acgccgttgg tcacctgttc acgcagctgt tgcgtatcga gtgcaaaacg atggtgaaag
    16261 tcgtaggcca gcaccttgcc ctgtttgcgc gcggtatcgc acatttcccg cgcctgttct
    16321 gccgtcatgg cgggcggttt ttcgcacatc acatggcagc ccgcttccag cgccatcagg
    16381 gtatgttcgt aatgaaaacg gttaggtgag cagacgctaa ccacatcagg tttcaccgcc
    16441 agcagcatgg cctgcggatc gtcccacacg gatgcattcc cgtatttttc tgccagcgcc
    16501 tgcgcctggg aaaggcggct gtcacagaca gccaccagtt ccagatcgtt gcgggtgcag
    16561 tagtacgaag catgaacttt atccgccacc tggcccgcgc ctattatcgc gacccgcagc
    16621 ggagagcttg tcattgcact tttcacaaag acctcttagc aggtacgcaa ccaggccaaa
    16681 gaatcacggt acgcctgggc tggatcttcc gcccggatgc gcccttcgta caccacatag
    16741 ccctgaaagt tatccgcgcg cagctgttct aacagcgcgt ggaaatccag ggtgccgctg
    16801 cccggctggt agcgatggtt atcggcaata tgcacatgac cgagcaggtc gcggttgtca
    16861 tgcagcgcct gcgccaggtt atcttcttcg atattcatgt gatagaaatc gccgataatc
    16921 tgtacatgtt taagatcgtt ttcgacgata taacggcggg catcggcgag agtgttgatc
    16981 atgtgatcct gataacggtt taacggttcg aggtacacca cggttccggt acgtgcggcc
    17041 accttttcca gtacgcgcag ggaatcactc accattttgc ggtcgccgtc caggctacgc
    17101 ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc atgcagccgg aacgacgata
    17161 cctttaccgc ccacttctgc cagagcttca agaatgcgtt cgatctgctt taagccatta
    17221 agtcgacgct cttcgataaa gtcgccaatc caaccgtcat agccaccgca ggcggtgatc
    17281 accggtaaac cggtttcttt gattgctgct ttgacttctt cgagattgtt gaccagcagt
    17341 ttgccgtcga tctcaaaacc atcgaaaccc atctctttga tataacgaaa tttctccaga
    17401 atgttttccg gaaagaacgc ctgattctgt gtgccgattt tcattttttt cattcctttg
    17461 aattaaaacg taacgcccat tttgatgctc tgttccggat gctggtcgac atactgcatg
    17521 taactttccg ggctattggc aaaggtcacc accgggtcga ttaaatcttc acaattgaga
    17581 taaccgttca tcagcagttc ccaacaggtt tcttcgatac gtttgcggct ccagcgcgga
    17641 taatccgggt tcggctcgct gcacgcacgg gagaaaacaa ttttggcgtt attgaaatgg
    17701 gcttcgcgtc cgaggttaaa accttcggca aacggtttgg caaacgcgac atacgagatg
    17761 gtgccgccat atgccagacc tcgtagcgcc gattgcagcg cgtcggcata gccgctggtt
    17821 tcgataatca catcggcacc ctgcttgccg gttagcgttt tgatctcttt gcctacgtca
    17881 ctgccaatgg ggttaaggca gaaatccgcg ccatggcgac gggcaatctc acagcgatgg
    17941 gcgataggat cgacgccaat caccaccgac gcgccagcac gtttagccag ttgaatggca
    18001 atttgaccga tcgcgccaag ccctaccacc accacaaaat cgcccacgcg cacattggca
    18061 tcgcgcacgc cgctcatggc aaactgcgcc gggtcgtagc agacagcgtt tttccacgaa
    18121 ctgccttgcg gcattttgcg cagcttgtag ttattcactg cgttaatgat gaccgtctcg
    18181 gagagcgggc cgtagccgca tacgctgtcg cccaccgcgt agtcggtaac gtcgctgccg
    18241 cactcgataa tgtcgccaac caccatgttg ccaagctgga atttgccaaa ctcaatgccg
    18301 cgcggcgcat ctgccggacg cggggtgaac atctgccatt cgccgttaaa gtcttcatcg
    18361 ataaacggac tggcagcgcg gaaatcgacc acttccgtcc cgtgcttcgg tgcgccgaag
    18421 cgcacgcgga tcttcacttc attggctaaa atcgcccgat cttcatactc aaccagcgca
    18481 gctacgcgcg gtgctgtggc tactaacttt ttcatgtaaa actccttgta aagaaactgg
    18541 ccgctttagc ccttcacgcc accggcggtc aaaccactct taataaaacg ctccgaaagc
    18601 gcatacataa tcaccaccgg caatgcggtc accagcgagg ccgccatcat ccgtccccag
    18661 atgtaatcag gcgtactgaa cagcgcgttc aggccgactg gtaaggtgaa attgctggcg
    18721 ctggagagga aaatcgacgc aaacagatag tcgttccatg ccaccataaa gcagtagacg
    18781 aatacggata tcagcccgga catcgccagg ggcacggtga tacggaagat gatttgcaga
    18841 cggttgaggc catccatcat cgccgcttct tcgatctcat cagggatggt gtcgaagtag
    18901 cttttcagca taaacacggc ggtgggcagg gtttgcgtta ccatggtaat gatcagcgcc
    18961 atctcggtgt cgtatatgcc aagcgcggtg atgattttga acagcggcac caccagcaaa
    19021 atgccggaga acatatacac cgtgtaaaag ctggcgttga tggtcatccg ccctttaaag
    19081 cgcaggcggg aaagcgcgta agccccgaga atgccgagaa ataccgccac cacggatgaa
    19141 accaccgaca ccaccatgct gttacggaag tagtcgacaa acgggaaaat catcgggtta
    19201 aaaatgtcga cataatgctc cagcgtccac tgttgcggca gcagcgtagg atgcagcgag
    19261 atcgcctctt ttgcgctctt gaacgaggtc atcagcatca caaaaaacgg aaacagcgtg
    19321 atgatgagaa acagcgccag cccgcagtaa aaaccgatgc gactgagtgt gcgtttattt
    19381 gttgccattg aggttcaccc gttttctggt cagcagaatg acagcgaaaa taatgatgaa
    19441 gagcaccacc gagatcgccg ccgctttccc taagtcatta aaggcaaagg cggttttgta
    19501 gagatagaca ccgagaatat cgaccttggt cgtcagcaga taaacatccg cgaacatgta
    19561 gaacatccag atggtgcgca gggtcaccac cgtcgccagg accggcataa tcgcgggcag
    19621 cgtgacgata cgaaaccgtt gccaggcatt agcgccatcc atctctgccg cttcgtacag
    19681 cgatttgtcg atggtctgca aaatcgccag aaacgaaata aaggcatagg ggaagtagcg
    19741 ccagatggcg aacagcacca ccagcacaaa gctactgccc ggattatcga accacagcgg
    19801 tgcctgctca tagagatgca gaagatcgac gccgaggtag ttcacaatgc cgtaaccgtt
    19861 gttgaacatg tatttccagg cgaacaccag cgaaatagac ggcgttacgt aggagaggat
    19921 caccagcgaa cgcgcggttt tgcgcaggcg gaactcgcgg ttgaaaaaca tcgccacggc
    19981 cagcccgagc acggtgctgc ccgccaccac cagcgcggta taccagaccg tcatccacag
    20041 cgagtgccag aagccgggat cggagagaat acgcacgtag ttgctcaccc caacaaacgt
    20101 tgactcgatg ttgggattaa gcggcagacg taaaaaactg atttcgatat tcgacaccat
    20161 cggccacgcc accagaccgc ccagcagtaa taagctgggc gcgagaagca acagcgcaaa
    20221 gggcatatcg gaacgacctg aaaaaagcct gttcattgcg gattcctgtc ctgggtttag
    20281 cgctgttcaa ccaactcatc cagctttttc tggctcgttt gcagcgtgcc agggaggtcg
    20341 gctttaccca cggtgacgtt atgcaccatg gagctcacca cgccagaacc cgtcacatcg
    20401 cccatgcggg taaaattttt atcccctact gcgccaaaga cctgaatatt tggcagttcg
    20461 ctgattaact gatttggcag ctcacccagc gccttaataa cgtcgttgtc tttccaggtg
    20521 gcggtagtga caaccgcttt gttcaccggc agcgccgcgc ctggcgacat catcacccag
    20581 tcggcaatgt tgtctgcctg ctccataaag gtgacaaatt tttcagccgc ttccgtctct
    20641 tcggcctttt gcccgacggt aatggtcagc gaggtcaaca tgccgtagac cgcagagttt
    20701 ttctcggtgg gtacaacgaa accaacgttt ttcggatcgc cttctttaat cacagccgga
    20761 aggatatagg tggagtaaat cgccatcggc gcggtgccgt tcataaaagc gtctttcact
    20821 tccatgatgt cgttagaacc tggcatggtg ttggcagcaa ggttgcggta ataggtcagc
    20881 gcctgcatca tctctggtgt atcaagggtg attttgcctt cggcgttaaa gacgttagcc
    20941 tgattggata acgcaaactg ggagaaagac tgttccgtca acacgctttc tgctgtaggc
    21001 agcgcaatac cgtatttttt attcgccggg tcattcagtt tctgtgcaac gtccagcaat
    21061 tgctgccagt tttttggctc ctccagccca gcttttgcca gcacatcttt gcgataccag
    21121 atcccgccaa tccatgcgct gacaggaaca ccggtccagg cgctgccatc ttcggtgcgc
    21181 acaatccgca gtacgccatc gtagaacgcg ccttcaccga cgttgctgat gaccgtggca
    21241 accgctttac gatcgataag ctgctctttg tccatcactt tggcgtagtc atggctggtt
    21301 tcgatcactt ccggcagcga accgctacgt gaaagggtaa tgactttggt gttataggcg
    21361 tcttcttcca cgggcacttg ttttacggtg atacctgggt tttctttttc aaaacgggca
    21421 atcaatttgc tgatgacggc ctggcgctcc tgctccaccg aagaatgcat aaattcgatg
    21481 gatacattat ttttattttc ttctttacag cctgaaatca gggcgcagga aaccagtgct
    21541 gataacagca cgatttttga tttaatcatg aaatgttcct ttttatttaa tccacataac
    21601 ctgccaggga tgcagtgtta tttcattacc caatatatca acttcactaa tcagatcccg
    21661 accgtgcagg tcagtaatat ttatatgctg aatattttca ctgacattaa acaatcccgt
    21721 caggcaatcg ccatccgcat tgcttctttg aattcgcatt acaaatgaat taatcgcatc
    21781 aatggtgaaa ttattatccg gatgaaactc tttatgactg cgacgaagtg taattaaatg
    21841 cgacaactta taatatatca aataccttaa tgtcgcttta ttgttcagtt ctgtcgttat
    21901 ctctttacta gagtattttt tacggttaat cgcacgatta tatccaagtt tttcgacacc
    21961 tgcgtaatca ttacgcgacc ccagaatgct ttgaatatat atcgctggca caccggggaa
    22021 acttaacaaa atcgcatggg cgagaataaa cctggcacac cgttcttcgt cgctacactc
    22081 acggcggctc aacgcatcca tataggtgac attcatttca taggggctac gcgtaccgtc
    22141 gggattattt ttccagttta ctaatgcccc ttcctgctgt aacacctcga ccagcgctaa
    22201 tatttcgttt tcgggcaaca acccccgcag tgggttaagc ccgatgccat cgtgagaggc
    22261 aaggaagtta aaccaggtgg tgttgctgga aggtagtgtc aggttttgcg cccacgcaca
    22321 aagtgcctca acgttctgtt tttgcaccgc atgcagcacc agcggcggca gcgagaactg
    22381 gtacaccata tgcgcttcgt catcaccgtt gccaaagtaa gcaatgttgt ctatatgcgg
    22441 aacattggtc tcggtaatga tcactgtgcc tggcgcaacg ttatcaataa tcgaccgtaa
    22501 cagtttgata atcagatgtg ttttttccag atggatgcag cttgttcccg gctctttcca
    22561 cataaagcca acggcatcca gacggatata ctcagcacct ttctcaaggt aacacagtaa
    22621 aacatccacc atcgccagta acacttcagg gctacggtaa ttgaggtcaa tttgatcgtc
    22681 gctaaaggtg gtccataaat gacgcgttga attatcgcgc atctggaatg gcgttaataa
    22741 cggtaacgca cgcggacggg tgacggcgct gagatcggtt tgcggatcaa ccgcaataaa
    22801 aaaatcttca aaacctggct gctgttgtaa atagttttta aaccattcac tttttgccga
    22861 catatggttg cagacaaaat caaacattaa atgactgcat tcaccgagtt gctgaatatc
    22921 ctgccactcc cccgcttcac tggcgacctg atgataatca attaccgaaa agccatcatc
    22981 agatgaccat ggataaaacg gcaacaaatg aacatgtgaa aaagtgcttt gcagccattg
    23041 atggtaaaac tgattaaatg tgggtagtgg ttttaaatca ttactgtgaa attgatcggc
    23101 ataggtaatt aacacgacat cactttcatc ccagtgtttt ttacgtcgct gtgtaattaa
    23161 tcgtttcgca ctctcaagac gcgttacaag tttctgtaaa tgagtcgcgg taaatgttcc
    23221 accgtagatt tcgtccaggt aatccgtaat tttctgttcc atgttgcctt ttcgtggtag
    23281 cggtcccatg aaacgagaga ctactccgca gaaaataaac tgtcaattga gcacaagggt
    23341 tttagtcacg caggaaatca ttttttacca gggaaaaagc gtatttgtga gctgacgcaa
    23401 gtaatctatg gggatgtaaa taaggtgtga cagaaaaagt aaacggcgca taagcgccgc
    23461 ttagggtgga ttcttttaac ctttgacctt cggcattgcg atgtctttca ggccgccggc
    23521 gttctcaacg tgggtatatc ccatctcgct aaggatctct tttgcctgcc ctgactggcg
    23581 tccggcattg caatacactt tcacggtgtc gtttttatcc ggaacggcgg tggcaatgcg
    23641 ctctttcact tccttcaggg gaatattgat ggctccctga acgtgctctt gctgatactg
    23701 ctctggaaca cgaacatcga tccagtgttc agcggcgaaa acgggcagtg aaaacaccag
    23761 tgtcagagca agtaagcctt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc
    23821 atccataata cgccttagct ttaacaattt ttgcgaaact cccctcacct tttataacgc
    23881 tgcgccagtt tattggcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt
    23941 ttcagtggtc ggcgagcaac tgattttatc gcccagccag ctacacccgc cgggccatag
    24001 cgcagtgcgg taagaagtac cagcttgcct gctaatttga aaccaggctt caccttttgc
    24061 ccggcctgtt gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta
    24121 acgtgaaagt atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg
    24181 ccagttcacg gtcgacttca tccagcaatt cactgctgga aggtagctgc tcaccaaagg
    24241 ccatgttgtc cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa
    24301 acagcgccag accgaagaaa atcgacagca ccaccaggat acgcaccagt tttactggta
    24361 catcaaaata gttggcaatc ccggcgcaga cgccacggac catgccctgc tgtggaatac
    24421 gccataattt tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc
    24481 tgcatcaaga atggattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcagc
    24541 cagttgcgct aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct
    24601 gtaatgcagc cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc
    24661 cagaaatagc gcgctcataa ggactcctta gacgatgagc ggcggcgcgt tcggacgccg
    24721 cctggatgtt attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgctcg
    24781 ctgattgcat catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg
    24841 aagctgtggc tttctgcttc cgcttccatc tggtcaattc gacgttcgaa agattcgaaa
    24901 cgagccattg cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag
    24961 tttgccgcct gatggcgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg
    25021 ttttccagct cgccgatctc tttcttcatg cgtgccagcg tatcgtccac cagcgtcact
    25081 tcatgttcca ggctcttaat cagatcggtc agtttctgtt tttcaattaa cgccgcacgc
    25141 gccaggtctt ctctctcttt cagcagcgcc agttcggctt tttcctgcca ttcaacttca
    25201 cgcgccgacg cttgttcaat acggcgagtc agctgtttct tttctgccag cgcacgcgcc
    25261 gaagtagaac gtacttcaac cagtgtatct tccatctcct ggatcatcag acgcaccagt
    25321 ttctgtggat cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca
    25381 aagcgagaaa aaatacccat aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc
    25441 tgaactatta atacaatttg cgtgccaatt ttttatcttt ttgatttata aagatctgat
    25501 tgaagaatca acagcaacat gccaggatga gttagcgaat tacactaaca agtggcgaat
    25561 ttcatcatgg cagaatacaa agataattta ctcggtgagg cgaacagctt tctcgaagtg
    25621 ctggaacagg tttcgcatct cgcaccgctg gataaaccgg tgctcatcat cggcgagcgc
    25681 ggcacaggta aagagctgat tgccagccgt ctgcattatc tctcctcccg ttggcagggg
    25741 ccgtttattt cccttaactg cgcggcgtta aatgaaaatc tgctggattc cgaactgttt
    25801 ggtcacgaag cgggggcgtt taccggtgcg caaaaacgtc atccagggag atttgaacgt
    25861 gccgacggcg gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggag
    25921 aaattattgc gcgtgattga gtacggtgaa ctggagcgcg ttggcggcag ccagccattg
    25981 caggtgaatg tacggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa
    26041 ggtacttttc gcgctgacct gctcgaccgg ctggctttcg atgtagtgca actgccgccc
    26101 ctgcgcgagc gcgaaagcga cattatgctg atggcagaac actttgccat tcagatgtgt
    26161 cgggaaatca agctgcctct gttcccgggt tttacggagc gcgccagaga aacattgctg
    26221 aattatcgct ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcggtgtat
    26281 cgccacggca ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt
    26341 ccgcctgaag acgctatcgc cgtttcagaa accacctcgc ttccaacgct gccgctggat
    26401 ttacgtgagt ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacaaggg
    26461 aaatataacc agaagcgcgc ggctgaatta ctggggttaa cctatcatca gttccgcgcg
    26521 ttgttgaaaa agcaccagat ttagcgaagt tgggcaatac aacttgccgg atgcggcgtg
    26581 aacgccttat ccggcataca taatcaatac gttgtttgca ggcctgataa gcgcagcgca
    26641 tcaaacatta ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc
    26701 tcatcgaatt gcgcatccgg gatggtcagc ggatacagga agcgaatcac gttgccgtat
    26761 gcgccacagg tcagcaagag cagcccctgc gccagcgcgc gttgctggat tttctgtgca
    26821 atcgccgctg acggctcgcc cgtttgcgga tcgttaaact ctgccgcaat catagacccc
    26881 agaccgcgta ccgccgcgat ggccggaacg ctttctttgg catcaatcaa cgtgtttgtc
    26941 aggcgctggc ccagttgatt cgcgcgttca cagagtgatt ctttgtcgat aatgttgagc
    27001 acagcgtgcg cggcagccac cgccagcggg ttcccggcgt aggtaccacc caacccgccc
    27061 ggcgcgggcg cgtccataat attcgcgtta ccgaccacgc ccgaaagcgg catcccgcct
    27121 gcgaggcttt tcgccatcgt cattaagtcc ggcttatcgg cgtagtgatc catggcaaac
    27181 attttaccgg tacgcgcaaa gccgctttgc acttcatcgg caatcatcac aatgccgtgc
    27241 tcgtcgcaca ggcggcgaat ggcggcaacc agctcttttg gcgcaacgtt gaaaccgcct
    27301 tcgccctgaa ccggttcgaa aataatcgcc gccacctgct tcgcttcaat gtccgattta
    27361 aacaagcgtt cgatggcgtc gagggagtcc tgcgttgaaa tgccgtgtaa atctgacgga
    27421 taaggtacgt gatataccga accggggaac gggccgaagc cgattttgta cggcgcgacc
    27481 tttccggtca gcgccatggt catatatgta cgaccgtgga agccgccgct aaacgcaatc
    27541 acgccagggc gtccggtatg ggcgcgggcg attttcaccg cgttctccac cgcttccgca
    27601 ccggtggtga agaacgcagt tttagcctgc ccgctcaccg gggcaagggc gttaattttc
    27661 tccgccagcg tgacgtagct ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt
    27721 tgcaattgct gttccaccgc cgcgaccaga tcagggtggc gatgcccggt attcagtacc
    27781 gcaatgcctg cggcgaaatc gatatattcg ttgccctcta catccttcag cgtggcgttt
    27841 tctgccgact gggcgaagaa gttacacatc acgccaaccc cgcgtggggt agcagaaaga
    27901 cgacgctgat ggaattcatt gttgctcatg tttcacacct tttgactctt cactgttaat
    27961 cggaaattaa acgcccagac gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa
    28021 cggaacgcgc agcgtgcgcc cgccggggaa cgggtaatgc ggcagattgg cgaaggcgtc
    28081 gaaacgctcg gcgtcaccgc gcagcagttc agcaatcaaa cgcccggcga ggtgggtaca
    28141 ggtgacgccg tggccgctgt agccctgcat gtagtagatg ttggtatcga ggcgaccaaa
    28201 ctgcggcatg cgtgacaggg tcagcaggaa gttgcccgtc cagcggtaat caattttcac
    28261 gcccttcagt tgcgggaagg ttttcagcag tttcggcacc acaaggcgct caacatcgtc
    28321 cgggtcgcgt gcgccgtaga ccacgccgcc gccgtacagc aggcggttat cggcggtaag
    28381 acggtagtaa tcaagcagat agttacagtc ttccacacag tagtttttcg ggatcagcga
    28441 acgggctaaa tcttccgaca gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct
    28501 gcgtttcgct agttccggct ctactttatc gcccaggtag gcattcccgg cgacaatcac
    28561 atacttcgcc gtcacctgac cattggcagt ccgcaccacg gctggcgtgg tgtgctggat
    28621 ctgcgtcact gcggaaagct catgcacgcg cccgccgttc aggcggatgg catcggcttc
    28681 accaatggcg aggtttagcg ggtgaatatg cccaccgctg tgatccagca gcgcaccagt
    28741 gtagcgatcg ctggcgacct cgcggcgaat agcgttggcg tccagcagct ccagctgttt
    28801 attgccgtag cgttcccagt tctctttctg ctcttcgagt gtggcgagct gtttatcatt
    28861 catcgccaca aacagaccgc cggggcgata gtcgcagtca atctgataac gtttgatacg
    28921 ttcgcggatg atctcgccac cctcgaacat catgctgccg agcatacggg cggtatccat
    28981 gccgtagctt ttttcgatca cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc
    29041 gttgcgtccg ctcgcgccaa agccgatgcg tgaggcttcg agaaccacca cgtcaaagcc
    29101 cgcttccgcc agatgcagcg cggaggaaag cccggtatag ccgccgccga ccacgcaaac
    29161 gtcgcaggaa atcgactcat tcagcgtgtc gaatggcgcg tatttattcg cactggcggc
    29221 gtagtaactg ctggtgtgtt cggtcatttc aggcctccag gcttatccag atggttttca
    29281 gttcggtgaa tttttcaagg gcatgcaggg atttgtcgcg cccgttgccg ctctgcttat
    29341 agccgccaaa cggcacggtc atatcgccgt cgttgtagtt attgacgaag acggagccgg
    29401 ctttcaggcg gcgactcatg cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc
    29461 caagtccgta ctggctgtcg ttggcaagct gtagcgcctg ttcttctgat gtgaaacgcg
    29521 tgaccaccag caccggaccg aaaatctctt cgcggcttaa ggacgcattc gggtctacct
    29581 ccacaaagat ggtcgggccg atggcggcag ccagctcggc gttacggcca tccagcagca
    29641 gttgcccttt gctttcgcct tcccgaataa agctatggac cgagtcggcg tgggcgcagt
    29701 cgattaaggt gcccatggtg gttgcgggat caagcggatg gcccggctgc cagttttgcg
    29761 cttgctgttt taacagggct aagaattcat cggcgatgct ctcttccagc aacaggcgcg
    29821 ttccggcgat acacacctgc ccctggttgt agaaaatgcc cgcagcggtc gcgctcgccg
    29881 ctttttgcaa atccggacag tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca
    29941 gccagacgcg tttcatgttg ctgtcgcccg cgtccttcag cagctgtttc ccggtgcggg
    30001 ttgagccagt aaaggcaatg gcatcgatat cgttatggcg cgaaagcgcc tgcccggctt
    30061 catgaccaaa acccgtcacc acgttcaaca caccatccgg cagccccgct tctttcgcca
    30121 gccccgcgag acgaatcgca ctgagcggtg atttttcaga cggttttaga atcacgctgt
    30181 ttcccgccgc cagcgccggg ccgagtttcc agcaagtcag cagcagcggg aagttccacg
    30241 gcacgatggc ggcaatcacg ccgaccggtt cacgcacgat catcgccagc tcatggctac
    30301 tggtggtcgc cacttcgcca tacactttgt cgatcgcttc ggcgtaccag cgaatggcgc
    30361 gcgccgcgcc gggaatatca tcacgcaggc tgtgacgaat cggtttaccg gtgtcgagcg
    30421 tttccagcag cgccagttct tcagcgttgg cttccattaa atcggcgagt ttattcagca
    30481 ccgctttacg cttagccgga gaagagagtg accagtcgcc acgttcaaac acgccgcgtg
    30541 ctgcgctcac cgcacggtca atatcgacgc tcttgccgcg ggcaatattc gccagcggtg
    30601 cctgggtgac cggatcaacg gtttcaaaag tttcattttc cgccgcagca gtatattcac
    30661 cgttaataaa taagcgggtt tcaatggcga gacttaacgc tttatcctgc cagtaagcca
    30721 gatgatgaaa attcattatg actcctgttt cacgtctatc agatatatgc agtttgtgaa
    30781 cgtcagggcg cagagatccc cgggccgtat gcccgtttat ttctggcgac attctttata
    30841 gattgccggg cgcattgcgc ttacccggct tacagaacaa aaagattaaa acgtggtggg
    30901 cgtatgggcg ctgataattc ggcaaatacc tgccgacgta ttactgaaac tgtgcgggat
    30961 gccggtatta atggcataac tttgccccgc gacgagatga taatcctgac cattaatcgt
    31021 cagaacaatt tcaccttcca gtacagtgcc tatttcctca ccctgatgct taattctttc
    31081 cccagtggtt gtgcccggct ggtaagtttc aaagatcatc gccagcgtgc gattcgggtt
    31141 accgttatga accagcttca ttgacacacc ctgactgccc atctcaatta agtcgtcctg
    31201 attaatgacg acctgcggct catcaggttt ttccggctcg gaaaagaatt ccgagagtga
    31261 cagaccatac accttcagca gcttttgcag cgtactgatg gcagggctga ctttatcttg
    31321 ttctatcgta ctgatagcac tgtgagtcag cccggagagt tcggcggcac gacgttgtga
    31381 aagcccctgc tgctggcgga tttccgacaa gcgttttcct ggcgccagtc cctcatcact
    31441 catatttgca tttccttaaa ctgtagtggt cagagtcgct gtttttcagc gatatggtgc
    31501 tgacaagcgg tgataaagcc ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc
    31561 cattccgggt gccactgtac gcccagcgca aaaggatgat tgatgacgct aaccgcctca
    31621 actaagccat ccggcgagcg agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct
    31681 tgtccatgca gagagtttac ccaaaagttg ctacattcag gtaacaacgc agacagtaat
    31741 cccccctctt caacctgaac ttcatgcgac ggcgcatatt gctgctccac cggcagttcg
    31801 ggatcttccc gatgttccag caattcaggc tgttcgcaca gcttgcgatg caacgaacca
    31861 ccggttgcca ccaccagttc ctgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt
    31921 tcgagtgcgg cattaattaa cgccatgctc agaagatcac gcccaggatc ggcgtcaggc
    31981 tcatcgccgt tttcaccata taggtgcggc tgcacattgc tgggactacc aggaagataa
    32041 atgccatcga gtttcggcaa aagttgttca agtaatgacg gttccgccag cgcatgtggc
    32101 agcgcaattg gcaagccgcc tgcgtggatg atggcattca ggtacttttc ttgcagagtc
    32161 tgggtcgcat gacccttaag cctgttcctg cacattacga caccgataac cggattgttc
    32221 attatatttt ccatgctcaa tctcacaaag tggactaaat tatcgccatt actgcgcttt
    32281 aaatgacctt tatgttcaat attttttcaa tctagcagtg ggttttcatt tttgcaaact
    32341 caatttaaca tttgacaaac atttagtttg catacagatt cgaatagtgg tcattatatt
    32401 ttacgctttg ataacgagcg gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc
    32461 gttgaagtag agaactttgt tcagcagtca gaagagaggc ggggtagctc ctttacgcag
    32521 gaagtgaaac gctacctgga gcgctacccg aatacgcaat atgttgatgt tttacttacc
    32581 gatttgaatg gttgcttccg tggaaaacgt attcctgttt caagcctgaa gaagctcgag
    32641 aaagggtgtt atttcccggc ctcggtgttt gcaatggata ttctgggcaa cgtagtagaa
    32701 gaagcgggcc tgggtcagga aatgggcgag ccggatcgta cctgtgttcc tgttctcggt
    32761 tccttaaccc cttctgccgc cgatccagag tttatcggtc agatgctcct gaccatggtc
    32821 gatgaagatg gcgctccctt tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag
    32881 cagctgcgcc agcgcggatt gttcccggtc gtagcggtag agctggagtt ctatttactg
    32941 gatcgccagc gcgacgctga aggatatctg caaccgccct gcgcgcccgg caccgatgac
    33001 cgcaatacac aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc
    33061 aatgatattg atgagctggc gcagttacag ctgattccgg cagatggcgc ggtcgccgag
    33121 gcctcgccag gtcagtttga aatcaatctt taccataccg ataacgtgct ggaagcttgc
    33181 gatgatgcgc tggcactaaa acgcctggtg cgtctgatgg cagaaaagca taagatgcac
    33241 gccactttta tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc
    33301 agtatgcaaa ataatcgtgg cgagaacgtg ctttctgacg cggaaggcga agattcgccg
    33361 ctgctgaaaa agatgctcgc cgggatgatt gacctgatgc cgtcatcaat ggcgttgctg
    33421 gcaccaaacg tgaactcgta tcgccgcttc cagccgggga tgtatgtacc gacgcaggcg
    33481 tcgtggggcc ataacaaccg caccgtcgcc ctgcgtattc cctgcggcga tcgccataat
    33541 caccgcgtgg aatatcgcgt ggcgggtgcc gatgccaacc cgtatctggt gatggcggcg
    33601 atttttgccg gtattttgca tggccttgat aacgaactgc cattacagga agaagtcgaa
    33661 ggtaacgggc tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt
    33721 gagtttatcg agaatgatca cctgcgccgc tatttaggcg aacgcttctg ccatgtctat
    33781 cacgcctgta aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt
    33841 gagtggatgt tgaaaaacgc gtaatacccc ttgcggggtt gtttcatcgg cctgagcgga
    33901 gccaggcctg acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca
    33961 ggcggcaacg ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat
    34021 gattaaatcc ccgcatggcg agggaggctg cgtatgcgcc cctcctgcga ctgactggac
    34081 tccgccccct ctgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac
    34141 cttattacgc aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc
    34201 aacctggcaa aacccgtctg cgaaaatcac tgaaattgtg gcaggtggtg atgatgggtc
    34261 tggcctatct cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg
    34321 acggtcacgt tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca
    34381 gctacggcaa actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa
    34441 agtcgattaa cccgcacgtc ggatttatgg tcggctggtc atcgctgctg gattatctct
    34501 ttttgccgat gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag
    34561 tgccgccgtg ggtgtgggtg gtaaccttcg ttgccatttt aaccgccgcg aatctgaaga
    34621 gcgtcaacct ggtcgctaac ttcaatacct tgtttgtact ggtgcaaatc tccatcatgg
    34681 tggtgtttat cttcctggtt gttcagggac tgcataaagg agaaggcgtt ggcaccgtct
    34741 ggtcacttca gccgtttatc agcgagaacg cgcacctgat cccaattatt accggggcga
    34801 cgattgtctg tttctccttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc
    34861 cggacgccgc acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta
    34921 tctttatcgc tgcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag
    34981 acccggacgc cgcactgcct gaaattgcgc tatacgtcgg cggcaagctc ttccagtcga
    35041 ttttcctctg taccacgttt gtgaacacgt tagcgtctgg cctggcgtcg cacgccagcg
    35101 tgtcgcgtct gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct
    35161 atgtgcaccc gaaatggcgg actccggcac tgaacgtcat tatggtcggg attgtcgcgt
    35221 tgtcagcgct gttctttgat ttagtcaccg cgacagcatt gattaacttc ggtgctctgg
    35281 tggcatttac cttcgtaaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga
    35341 ataaaagctg gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg
    35401 tgggtgtgtt gtgggttaac ctcgagtcaa cgtcactgac actcggtctg gtatgggctt
    35461 cgttaggcgg cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgttgtatg
    35521 atggtgacag aacgccggtg agcgaaacgt aatagtcgga tgcgcggcgc attatcctgc
    35581 ctgagggtaa tgcaccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc
    35641 tatcatccgc ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc
    35701 ccgctaaaat attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca
    35761 gacgctgaac gcacaccgct ggccctctac ttccagttac tgctcacccg tttgatgaac
    35821 aatgaagaga tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc
    35881 gtacgcattg atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttcat
    35941 tgcagactct cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca
    36001 atgacacgca ctgcagcgtg cctcaatggg tttctcacga tcgtaccgct ctgctcctcg
    36061 ctaagttgtt ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg
    36121 gcgacgtcat ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac
    36181 ggtaatggca aatgtcatta aatattagta agatagcctc tttacataaa atccattaat
    36241 attatgcgcc aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca
    36301 atcgccgcgc ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc
    36361 gtcagcgggc aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat
    36421 acccttgccg cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg
    36481 atgccggaac ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac
    36541 ctgcgtcgcg atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat
    36601 gccgacgatg tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac
    36661 gtcaacggca gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc
    36721 gtccgtaaac tggataatca taccgttgag ttccgtctgg ctcagccgga tgcttctttt
    36781 ttgtggcacc tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta
    36841 gagaaagaag atcgccagga gcaactcgac cgtcagccgg tcggcactgg accgtatcag
    36901 ttgtcggaat accgcgccgg gcaatatatt cgcttacaac gtcatgatga tttctggcgt
    36961 ggtaagccgt tgatgccaca agtggtggtg gatttaggct ctggcggtac aggacgattg
    37021 tcgaaactcc tgaccgggga gtgcgacgtt ctggcctggc ctgctgccag tcagctatcc
    37081 attttgcgtg acgacccacg cttgcgttta acgctgcgcc cggggatgaa cgtcgcctat
    37141 ctggcgttta acaccgctaa accgccgcta aataaccccg ctgtccgcca tgcgctggca
    37201 ctggcgatta ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg
    37261 gcctctattt taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac
    37321 aatccggcaa aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa
    37381 ctgtgggtgc caacacgttc gcaggcgtgg aaccccagtc cgctgaaaac cgccgaactg
    37441 attcaggcgg atatggcaca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgt
    37501 tttcaggagg cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg
    37561 gacagtaacg acccggacag tttcttccgt ccgttactga gctgcgcggc aattcattca
    37621 cagaccaacc tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc
    37681 tcctcgcagc agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg
    37741 caggaattac ccattctgcc gctggcgtcg tcattgcgct tgcaggccta tcggtacgat
    37801 atcaaaggtc tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag
    37861 aaacaggatg aggtgaaaaa gccatgatta tcttcacctt gcgccgtatt ttgttattga
    37921 ttgtcacctt gttcctgctg accttcgttg gcttcagctt gagctatttc accccacatg
    37981 cgccgttaca aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc
    38041 actgggattt tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg
    38101 tcttcccggc gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca
    38161 tccccgtagg aatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg
    38221 ccatcgcctt actgggcttt tcaatcccgg tgttctggct ggcgcttctg ctgacgctgt
    38281 tttgttcact cacactaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag
    38341 tgaaaccgat taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg
    38401 atgaaatggt catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg
    38461 cgccaacaac tgaagtgatc cgactgatgc gtattagcac tatcgaagtg tatgaccaga
    38521 actatgttaa agcagcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg
    38581 ttctgcataa cgcgttacct ccggttattc ctcgcctggg cttacagttt tctaccatgc
    38641 tgacgctagc gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa
    38701 ttaacgccat ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct
    38761 cactggtcat tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga
    38821 aacataagga atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg
    38881 ctgcgtaccg cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc
    38941 tgcgcggggc tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac
    39001 cagcaatttc tcggttacca attattgccg ccgtcatggt cgcgctatgg cgaagtttct
    39061 ttcttcctgg ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct
    39121 gcgccaaccg taggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggtt
    39181 ctgggcacct tcgccggggc gacccacggc ctacgctcag cggtgcttaa ccatattctg
    39241 gataccttac tggcgatccc ttcgctgctg ctggcaatta tcgttgtagc gtttgccgga
    39301 ccgagtttgt ctcacgccat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt
    39361 tcgatttaca gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg
    39421 gatggcgcat caacgctgaa tattctctgg tttgctgtga tgcccaacat caccgccggg
    39481 ctggtcaccg agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc
    39541 tttctcgatc tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg
    39601 ctggaactga tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt
    39661 agcgtgctgt tggttaacct ccttggcgac ggtgttcgtc gtgcaattat tgcgggggtg
    39721 gaataatgcc attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt
    39781 gggttaaagc cgtcgaccgc gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg
    39841 ttggcgaatc cggttccggc aaaagtttga ttgcgaaagc gatttgtggg gtgaataaag
    39901 ataactggcg tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct
    39961 ccgcacgcga acggcgcaag ctggttggtc acaacgtgtc gatgattttc caggaaccgc
    40021 agtcttgtct tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct
    40081 ggacgtataa aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac
    40141 tactgcaccg tgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt
    40201 tgaccgaagg tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc
    40261 tgctgattgc tgacgaaccg acaaactcaa tggagccaac aacccaggcg caaatctttc
    40321 gcctgctgac gcgtctcaac cagaacagca ataccactat tttgcttatc agccatgact
    40381 tacaaatgct tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacggtgg
    40441 aaaccgcgcc aagtaaggag ttggtgacaa tgccacatca tccttatacc caggcgctga
    40501 tccgcgcgat accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc
    40561 ccggcgctat cccactgctg gaacagttac cgattgggtg tcgtctgggg ccgcgttgcc
    40621 cgtatgcaca acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct
    40681 atgcctgtca cttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa
    40741 gtgcgtaatt taagtaaaac gttccgttac cggaccggct ggtttcgtcg tcagaccgta
    40801 gaagcggtaa aacctttgag ctttacgcta cgtgaaggcc agacactggc gattattggt
    40861 gagaatgggt cgggtaaatc cacactggca aaaatgctgg cgggaatgat agaaccgacc
    40921 agcggcgagt tattgattga cgatcatccg ctgcattttg gcgattattc cttccgtagt
    40981 cagcgtattc gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc
    41041 tcgcagattc ttgattttcc actgcgcttg aacaccgatc tggaacctga acagcgtcgc
    41101 aaacagatta ttgaaacgat gcggatggtt gggctgctac cggatcacgt cagttactat
    41161 ccgcatatgt tggcgcccgg acaaaaacaa cgtctgggtc tggcacgcgc gctgatattg
    41221 cgtccaaaag tgattattgc cgatgaagcc ctcgcctcac tggatatgtc gatgcgttcg
    41281 cagttgatta atctgatgct ggaattacag gaaaaacagg gtatttcgta tatttatgtt
    41341 acccagcata tcggaatgat gaagcacatt agcgaccagg tactggtgat gcatcagggc
    41401 gaggttgtcg agcgaggcag caccgccgat gtgctggcat cgccgctgca tgaactcacc
    41461 aaacggctta ttgccggtca ttttggcgag gcattgacgg cggatgcgtg gcgtaaggac
    41521 cgttagtggt ttgcacctca tcctgacagg gcccccctca ggatgaggtg ttcattactt
    41581 ttgattgtcg attctggtga gagcaaccag caatgccagc gtataacaca ccgtaattgc
    41641 cagactcatt tttaacagtg tttctccgcc aattcccgac aatggcgcgg ctataccgcc
    41701 gaagacaaac atgctcatcc ccatcaacgc agacgccgtt ccagattcct gcgtccctac
    41761 tgcactcata gcctctgccc cgccaacagt gccgatgccg ctacaaaatg cgatggtgaa
    41821 aaataatgcc accagtgccg gaatgggcaa ttgtatccat gcgcataaga ccgtgagcaa
    41881 tgcacacaaa attgccgcta tcaggccacc tcgcaacaat gtcatcgcgt taatccgtcg
    41941 cgccaggcgc gagaagatcc atgaagcaat gataagtccg atgccgttaa ggccaaacac
    42001 caggctaaat tgcattggac taaagccaaa ttccttctgc aacacgaacg acgaagagcc
    42061 gatatatgca aacaaaccag ccagcataaa actttgaatc aggcaaaagc gtataaaacg
    42121 gcggttctgc accacgcttc gtccggttaa tagcaatgat gagccccttt tattttctgg
    42181 caaggtctca ttaataaata acaggcagcc cagcaacagt acggtgctaa tttcagccat
    42241 tacccagaat aaagtgcgcc agtcaaaagt gctaacaatg tacccgccca gcactggcga
    42301 caacaccggt gccaggccat tcaccgtcat taacagcgca aaaaactggg tcaacgttac
    42361 tccctgatat ttgtcacgag caatagaacg agagagcacc gaaccacccg cccccgcgat
    42421 cccttgaata aaacgccaga ccaccagcca gtaaatattg ttcgttgtcg cgcataaaat
    42481 ggaagaaaca ataaacaata gcaacgacaa gattaacgga cgtttacgcc ctattttgtc
    42541 gctcagaggg ccaaataaca gttgtccgac gccaagacca atcagtgatg cggtcagggt
    42601 taattgcgtt atggtcgtgg ttgccgccag ttgctcagac atctccggca aagccggcaa
    42661 ataaagatcg gtacacatcg ggccgatacc ggctaaaaga ccaagaatca atgcccatga
    42721 aagagagact ctagccatat tattccttac ttgagtgatg acataccgcc gcccagcgac
    42781 tgccagagcg tgattcggtt tgtaaaatca gtcagttgca gtgccagcag ctcctgctgt
    42841 gcgctccaca agctgcgctg cgccgtcaac acggtcaggt aatcaccaac acccgcctga
    42901 tagcgacgca agccaacatc gaccgtttgt tgctcagctt taacatactg gcgctgtgca
    42961 tccagttgct cttcaagcgt ggtccgccgc gccagtgcgt tagcgacatc tttaaatgcg
    43021 ctctgaacgg ttttttcata ggtggcaatg agcccacgct tttgtgcttc tgcgtaacga
    43081 agctgcgcca gattacttcc gccagtgaac agcggtaatg tgaccgatgg cgcaaatgac
    43141 cagatctgca ttccgtggct gaacaacgac gacaatgcgt cactgccaac accggcactg
    43201 gcagtcagcg aaatcgtcgg gaaaaagtta gcccgcgccg cgccaatatc tgcattggcg
    43261 ctcttcaaat tatgttcagc ttcctggata tccgggcgac gcagcagaac atcagatgat
    43321 actcccgcag gaaccagact tatcatttgt tccggcaggc tttccagtgt tcccggtagc
    43381 agattttccg caagcgttgt acctgccagt aagttcagcg catttttatc ctgcatcact
    43441 tgtgtctgat agctggcaac gctggcgcgc gcttgctgat aaacactcat cgcctcactg
    43501 acatctgtcg ctgccgccgt gccaatctgt tgctggcgct ggataatttt caatgagttt
    43561 tctgcactgg tcatcgtttc ttttgccagg gccaggttgc tgttatcagc cgccagtgtc
    43621 agccaggcgg tgctgatttc agcaatcagc gttaaacgag tgttctgggc tgtaaattca
    43681 ctggcaagcc aggtttcctg cgccgcccga gataaactct gattacgacc aaacagatct
    43741 aacgtataac tggacaccgt gccgtcagcc tcggcagttg ttcccgtacc attcgccagc
    43801 gaacgactgc gagtcgaact taaggccgca ttcaccgtcg gaaataacga cgcattagtt
    43861 tgcccataga gcgcccgtgc tgagtcaata tcagcaatcg ctttttgcac atcgcggtta
    43921 ctgttcagtg caatggttac tacctgctgc aatcggggat cgtggatcac ctgctgccag
    43981 tcatggctga ttgccttccc ctggccctgt gctcccggca aggtcgccgg gattggggat
    44041 tcaggcgtac tgtagtgggg atcgagcgag acacatcccg cactcaacaa caccagaaaa
    44101 ataagagaac gacgcaacat aacttactcc tgacggcgcg gtttaccggc aaacaaacgt
    44161 ttcaccagta caaaaaacag aggaacaaaa aaaatagcca gtaatgtagc ggtcagcgtg
    44221 ccgccaataa tgcccgtacc aatggcgatg cggctgttcg cccctgcacc ggttgctatc
    44281 gccagcggca ttacccccgc aataaacgcc agcgaggtca tgatgattgg gcgtaaacga
    44341 gtctgcgccg cgcgtaatgc cgcacgactc aaggaatagc cctctgcaac cgccgcttca
    44401 gcaaattcca caatcagaat ggcgtttttc gacgacaagc cgatagtggt taatagcgcc
    44461 acctggaagt aaacatcgtt gtttaaatca cgcatccagg ccgccagcgc cgcgccaaga
    44521 agccccagag ggatcaccag aatgaccgag aacgggactg accagctttc gtacagtgct
    44581 gcaaggcaga ggaaaaccac cagaatagaa actgcataca gactcaatgc ctgaccgctg
    44641 gccagtttct cctgcaatga caaaccactc caggcccagg ttgttcccgc aggaagactg
    44701 tttgccagtt cctccatttt tgtcattgca tcgccggaac taaagccagt cgcattttcc
    44761 ccctgaattt cataggctgc agaaccgttg tagcgcacca gtctttccgg tccatacagc
    44821 cagcgggtgg tcgcaaaagc agagaatggt gtcatggcgt tatcgctacc gcgcacaaac
    44881 catttaccta agtctgacgg cgcggaacga aattcactgt cgccctgaat gtagactttt
    44941 ttcacacgac cacggtcaat aaagtcattc acataagtac cgccccacgc actggacagg
    45001 gtgtcggtga catcattcaa actcaaccct aatgacaccg ctttattact atcaatatct
    45061 acctgcaatt gcggcatttg tggcaaatca ttggcacgca cagaatgtaa ctccggactt
    45121 tgattcgctt tttctatcaa ctgattacgc atttgcagta gtgtttcgcg atcggttcca
    45181 ccgttagcta acaattcaaa cgtaaaacca ttgctttggc ccagcccatc aacggctggc
    45241 ggcgtcatcg caaataccgt ggcatcacga attgtgccca gctctttggt agcccgtagg
    45301 gcgatagcct gggcggtgtt ttctgcccct ttacgttgag accagttttt caacgaaaca
    45361 aacgccatcc cggtgttctg tccgctgccg ctaaaactga aaccatcaac ggtaaaaatg
    45421 acatcggtat ttgctttctc gttaatcagg aaccagtcaa caatctggcg attcacttct
    45481 gctgtacggg cagcggtcgc cccggccggc agcgtgtact gcaccatgat ttcgccctgg
    45541 tcttcagtgg gtaagaaact gcccggcagt ttccacatca tcagggccat tccaccaccc
    45601 agtacgacat aaagccccat cgttcgagca gcacggcgca ggacataaat tacgcctcgc
    45661 tgatatttat cttcagtacg gcggtaaaag cggttaaatg cgccgaaaaa gcctttttta
    45721 tgtggcggaa catgctggag gacggaaccg cacagggcgg gagtcaaggt caatgccacc
    45781 accacggaaa gcagcattgc ggagatgatg gtgatggaga actgacgata aattacgccc
    45841 gtagatcccc caaagaaggc catcggcagg aatacggctg acaacaccag cgcaatggca
    45901 accagtgcgc cagaaatctc gcccattgat ttttccgtgg cttcacgcgc gggcaaccct
    45961 tcgtcacgca taatgcgttc gacgttctct accaccacaa tggcatcgtc caccagcaag
    46021 ccgatcgcca gcaccattgc aaacaacgtc agggtattaa tcgaatagcc aaacaacgca
    46081 agaacgccga atgtgcccag caaaacgacg gggacagcta acgccggaat cagtgtggca
    46141 cgcaggtttt gcaggaataa atacatcacg caaaccacca atacgatggc ttcaaacagc
    46201 gtctgaatta cgtcctctac ggagatttta ataaattcag tgctgtcttt cgggtatgcg
    46261 atgtcgtatc cctgcggcat gttccgctgg aattcggcaa tcttatcctt gaccagcgtc
    46321 gccgtattca gcgcattcgc accgggcgaa agcattacag caaccccggc agacgggtga
    46381 ccgttaagtt tgccgatagc ggtataatct tcactgccca tctcaacgcg agccacatct
    46441 tttatacgaa caactgcgcc gtctgactgg cttttcacga taatattttt gaactgatcc
    46501 accgtctgca aacgagactg cgcacgtacc gttgcggtca gttgctgagt attcggtgaa
    46561 ggcaatgccc ctattttccc ggcagtaacc tgcacgtttt gcgcttcaat agcactttgc
    46621 acgtctgaag gcattagcga gtaagacgcc agtttggccg ggtcaagcca gatgcgcata
    46681 gcgtattccg cgccaaagac ttgcagactc cccacacctt caacacgcgc cagcgggtcc
    46741 tgaacgttac taaccaacca gtcggcgata tccgaactgg aggctttgtc agtggtgtca
    46801 tacacggcag caatcagcaa aaagttgctt tgtgatttct ccaccgtaac acccgtttgc
    46861 tgcacttcgc tgggtaggcg cgactccgcc tgctgaattt tattctgcac ctgaacctgt
    46921 gcagtatctg gatcggtacc ttgttcaaag gtcacattaa tactgaccga accatcagag
    46981 ctgctggttg agctgaaata gagtaaatta tcaagcccag tgagttgttg ctcgataacc
    47041 tgagtcacgc tgttctccag cgtttcggca gaagcaccag tataagtggc tgaaatttta
    47101 atggtcggtg gcgcaacgtc aggatattgc gcgacgggca atgtgcgaat agccagaatc
    47161 cccgccaaca taatcaaaat ggcgataacc caggcaaaga ccgggcggcg aacaaaaaag
    47221 cgcgaaaaca tcaggcgttg cctccattag cctgaacttc aacagccttg acggtctgcc
    47281 ctgaagtgac tttggcagaa ccttcaacaa tcagtcggtc gccgttgtgc aggccgttca
    47341 gcaccagcca tttatcacca tacgtttctc ccgtttcgag cgttcgctgc tctactttat
    47401 tgtctttatt caccaccagc gcagttgcat tgcctttagc atcgcgcgtg acgccctgtt
    47461 gcggcgcgag aatagcgtct tccatcacgc cttcatcgac acgagcgcga acaaacattc
    47521 ccggtaataa ctgctgttgt ggattgggaa aaatcgcccg taatgtcacc gaaccggtag
    47581 actcatctac agcgacttcg gtgagttcca gacgcccttt ttcgctgtaa gttgtgccat
    47641 cttccagaat aagtgagacg ctcatggtgt cactgttggt cgccagtgac tgtttacgca
    47701 gacgtaataa atcgacacta gagcgagtga ggtcgacata cattgtatcc agaccacgaa
    47761 tagtcgttaa cgctgtatcc tgcgacgcgg tcaccagtgc gccaggggtt accgacgaaa
    47821 taccaatacg ccccgaaatc ggtgcggtta ccgtggtcca gtcaagatta atgcgcgcag
    47881 tttccagtgc ggcttttttc gcctctacac tggctttatc ttgtgcacag gtagactgag
    47941 catcatcagc atcctgttgt gaaacaccgt tctctttcac cagtcgggca taacgctgcg
    48001 ctttctggca atcggctttt accagtgcct gtgcttgttg taatgctgcc cgagcttcat
    48061 tccatgcagc ctgataactg gccgcatcaa tctggtagag cggctgtcca gccttgacca
    48121 gatcaccttc cttaaataag cgtttctgga taattccccc aacctgcgga cgtacttcag
    48181 cactgagcgc agcactggtg cgtccggtta attcactgac taccgaaacc ggttgagatt
    48241 tgagcgtaac aacgccgact tctgtttctg acggggatga attgttactt tgcgtattat
    48301 cacaacccga taagagcagc attgccacta cagatgttgc tatatacttc attattttct
    48361 ccagggtgag aaataccctc ctcattgaaa ttaatgagga tatgtcaggg atattcagtt
    48421 ataacgaacc cgcatcgcga aatgaatttc gcaatgaaat aagggggatt aatatccccc
    48481 gctattggtt aattctgttc ttatttattt atcaacatac taacaatatc ctgatatatt
    48541 tcctgcagat gttgtggatc gcttttttgc ggtgttaaac gacgataaac cgtaccttcc
    48601 atcatggtag caaacacttc aacgcagcaa cgaatgtgct cgtcgctaag atggggatgc
    48661 attcttttca tatgtgcaca accgttagcg aacattctgg cgtcagcttc ttcaagcatt
    48721 gccataaccc gcgggttgcg tgtggcttct gcagccactt ccagcatcag cgcgtcgtcg
    48781 tcctcattta aggatttacg caaggcaagg acttccggga agtgatcggt acgcgcgtca
    48841 atgtccattt gggcaatacg aaagtcgatg atacggcgaa ccatctcttc aataatggcg
    48901 tctttgttgg cgaagtaacg atatatctga cccacgctaa gctgagcttc gctggcaatt
    48961 tgcgacatac tggcggcatg aaatccgcag cgacgaaaac accgacgggc ggcgttaatg
    49021 atttcatcct gacgctgttt gtggcgtatc tccagcttgc tggtcatttc tgtctcctgg
    49081 cctatcactt gatgagaatg atcgttctca tttttgctaa ttatacgttt ctccaaagta
    49141 atggaaagta tttttatctt attcatgatg catattctga taatgaagag attgtggaga
    49201 ttctgtgaag atagttgaca atattaagtt ggggtcaatt catcagaaag acagcgactt
    49261 gttgtcgcca gttctgtttt catcatcaaa tttatcggag ctaatacgaa tagtttcata
    49321 gtcagattct ataagatatt tccccaacct cccccttgca ggcgtcagga gcagataacg
    49381 caaaatgctc tgtaccgcag cttctctccg ctacagaaag cgcaactata gccacccaca
    49441 gccaggttga ttataataac cgtttatctg ttcgtactgt ttactaaaac gacgaatcgc
    49501 ctgattttca ggcacaacaa gcatcaacaa taaggattaa agctatgggt tttctttccg
    49561 gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc catcgcctac ggtatcgctc
    49621 aggcgatgca ccgcgaagga gctgaactgg cattcaccta ccagaacgac aaactgaaag
    49681 gccgcgtaga agaatttgcc gctcaattgg gttctgacat cgttctgcag tgcgatgttg
    49741 ccgaagatac cagcatcgac accatgttcg ctgaactggg gaaagtttgg ccgaaatttg
    49801 acggtttcgt acactctatt ggttttgcac ctggcgatca gctggatggt gactatgtta
    49861 acgccgttac tcgtgaaggc ttcaaaattg cccacgacat cagctcctac agcttcgttg
    49921 caatggcaaa agcttgccgc tccatgctga atccgggttc tgccctgctg accctttcct
    49981 accttggcgc tgagcgcgct atcccgaact acaacgttat gggtctggca aaagcgtctc
    50041 tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg cgtgttaacg
    50101 ccatctctgc gggtccgatc cgtactctgg cggcttccgg tatcaaagac ttccgcaaaa
    50161 tgctggctca ttgcgaagcc gttaccccga ttcgccgtac cgttactatt gaagatgtgg
    50221 gtaactccgc ggcattcctg tgctccgatc tctctgccgg tatctccggt gaagtggtcc
    50281 acgttgacgg cggtttcagc atcgctgcaa tgaacgaact cgaactgaaa taatcgttct
    50341 gttggtcaag acgggcggcg ttctgccgcc cgttatctct gttatacctt tctgatattt
    50401 gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg atttccttta
    50461 cgaccaagga gcgcccatgg aaaaacgcca cattaccggc aaaagctact ggtatcatga
    50521 aacgcaatcc agtactgcgg agtatgacgt tctgcctctg gtcccggaag ccgcaaaggt
    50581 cagcgatccc tttctgctcg acgtgatcct tgatgaagaa acgctggccc ccttcctttc
    50641 atggctggtc cctgcgcgcg ttcttgcagt ggaattgttc cctgaccagc ttaccgtgac
    50701 ccgttcacag accttcaccg cttatgaacg cttgtcgacg gccctgacgg ttgctcaggt
    50761 ttgcggcgtc caacggttat gtaactacta ttcggcgcga cttacgccgc tccccgggcc
    50821 tgattcctcc agggaaagta atcatcggtt agcacaaatc acgcaatatg cccgccaact
    50881 ggctagctcg ccttctatta tcgacaaccg atcgcgccag catctgaatg acgtcggcct
    50941 aactgcctgg gactgtgtaa tcattaacca aatcattggt tttattggct ttcaggcgcg
    51001 gtcaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac ccggtctgga
    51061 gatacaaaac tatgccgacg cgtcactgtt tgctgatgaa tcaatacgct ggcgaagcag
    51121 ctatgaggtg gaaaaactcc ctgaagagca cacaaaaagt tcaactgcag aactttgcca
    51181 actggccgaa acactctctc tccaccctat ttcactttcc cttctcgaaa agttgctaaa
    51241 cagcacacgg gtcaatacac agccggataa tcagcttgcg gcgttgttat gcgcgcggat
    51301 aaatggcagt cctgcttgtt ttgccacctg tatgaattca tcaaatgaat ataaaaaaat
    51361 cagcgccctt ctgcgcaagg gcgaaaatga aattaaccaa tgggctgacc gtcattctgt
    51421 tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc gctttagcgc
    51481 cgcccagttc agcccattac tcgaacacga aaaatcatca acgcagatta ttaatctgct
    51541 ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg gtgagacata
    51601 ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa tggccaccaa
    51661 aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag caactgcatt
    51721 cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg
    51781 aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg
    51841 gcgaccgaat tatcgcggtt atccacagtg aaaaagaacg tgaatccgca gagccagaag
    51901 aactggttga accgtttctg actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc
    51961 tggccatcgt tcctgatcat ccactcttaa aagacgccat tccttgccgc gcagcccgtg
    52021 gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc
    52081 tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt ggtgacgacc
    52141 actttgtacc gtggtgggtt acccttgcgc gccataatct ggagaaagaa gcaccagacg
    52201 gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc gcgctggatt
    52261 ttgtcaccat cgacagtgcc agcacagaag atatggatga cgcccttttc gctaaggcgt
    52321 tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg tggattgctg
    52381 aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct
    52441 tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg
    52501 aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc attgaagata
    52561 atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat gacctggttt
    52621 ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt gctgaacaag
    52681 tccgtttgtt agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg
    52741 tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa gtgctggata
    52801 tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg attgccgcta
    52861 acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat aacgtgcata
    52921 tgggctttga tccggcgaat gccgacgcgt tggcagcgtt gctgaaaacg cacggtctgc
    52981 atgtcgatgc tgaagaagtg ctcacgctgg acggtttctg caaactgcgt cgtgaactgg
    53041 atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa
    53101 ttagcactga acccggtcct cactttggcc tcggtctgga ggcatacgcc acctggactt
    53161 cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg gttatcaaag
    53221 gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgag cgtcgccgtc
    53281 tcaaccgaat ggcagaacgt gatgttggcg actggttata cgcacgcttc ctgaaagaca
    53341 aagccgggac cgacacccgt ttcgcggcgg aaattgtcga tatcagccgt ggcggcatgc
    53401 gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc ttacacgctg
    53461 tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa ggtgaaacgg
    53521 cttacaaagt gactgacgtt attgacgtca ccattgccga agtccgcatg gaaacccgca
    53581 gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctta tgaatgggcc
    53641 gtttatttcc ccgctctacc ttcatcatat cccggcagtt ttttaactga ctttcgtttg
    53701 aaaactggcg tttttccaaa actggattac aaataaatat ataaaaaaaa caatgcgttt
    53761 agccgccgtt aatttcatgt aagtcatgtt gttcctttta tttaaccgcg gactccgctg
    53821 ttaaccggag gatatgcatc atgaaaaccg ttagggagtc cacaacgttg tacaactttc
    53881 tcggatcgca caatccatac tggcggttaa cggaaagcag cgatgttttg cgcttttcta
    53941 ccaccgaaac cacagaacct gaacgtacgt tgcagttatc tgccgaacag gctgctcgca
    54001 tcagggaaat gacggtcatc acctccagcc tgatgatgag tctgaccgtc gatgaaagcg
    54061 atctttctgt gcatctggta ggacgaaaaa tcaataaacg ggaatgggct ggcaacgcgt
    54121 ctgcatggca tgacacacca gcagttgctc gtgatttatc acacggactt tcctttgctg
    54181 agcaggtagt ttctgaagca cattccgcaa tagtgattct cgacagccgg gggaatatcc
    54241 aacgcttcaa tcggttatgt gaagattaca cagggttgaa agaacatgac gtcattgggc
    54301 aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc ggcatccagg cgcaataacc
    54361 gtgtattttt tcgaagcggc aatgcatatg aagtcgaact gtggatacca acacgtaaag
    54421 gccagcggct gtttctgttt cgcaataaat ttgtccacag cggcagtggc aagaacgaaa
    54481 tttttttaat ctgttccggc accgacatta ccgaagagcg ccgcgctcag gagcgactgc
    54541 gtattctggc aaataccgac agtatcaccg gactgccgaa tcgtaacgca atgcaggagt
    54601 taatcgatca cgccattaat caggcagata acaataaagt tggggttgtg tatcttgatt
    54661 tggataattt taaaaaggtc aacgacgctt atgggcattt gtttggtgac cagctattac
    54721 gcgacgtgtc attggccatt ttaagctgtc tcgaacatga tcaggtgttg gcgcgtccag
    54781 gtggcgatga gtttctggta ctggcatcca atacctcaca aagcgcgctg gaagcaatgg
    54841 catcacgaat tttgacccgc ttacgactcc ccttccgcat tggtttaatt gaagtttata
    54901 ccagctgttc agtgggtatt tcactctctc ccgaacatgg ttcggacagc gcggctatta
    54961 ttcgtcacgc cgacacagca atgtacacag cgaaggaagg cggacgagga caattttgcg
    55021 tttttactcc agaaatgaat cagcgggtat ttgaatatct ctggctggat accaacttgc
    55081 gtaaagcact ggaaaacgat cagttggtta ttcactatca accgaaaatc acctggcgtg
    55141 gcgaagtgcg cagtctggaa gcactagtac gttggcagtc acctgaacgt ggattgattc
    55201 caccgttgga cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt
    55261 gggtgattct cgatgtagta cgccaggtgg caaagtggcg ggataaaggt attaacctgc
    55321 gagtggcggt aaacatttct gcacgtcagc tcgccgatca aaccattttc accgccctga
    55381 aacaggttct ccaggaactc aattttgaat actgccctat tgatgttgaa ctgacggaga
    55441 gctgcctgat tgagaatgat gagctggcac tgtctgttat tcaacaattt agccaactag
    55501 gtgcgcaagt gcatctggac gattttggta ccggttactc ttcactttcg caactggcgc
    55561 gctttccgat cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac
    55621 ctgtctcgca gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg
    55681 tgatcgccga aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca
    55741 atgagcggca aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgttggt
    55801 ataaacgcta tctgaagcgc gcataagtag atgaatatat atacttagcg atatattccg
    55861 taattatata tcgtattccc ggatgataat tagccgttgc tgttaaacac ttctctttta
    55921 gcggcaattt ttgcattatt cattttccca cttttgctat aaggatgtcc ttgatgtctg
    55981 aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag
    56041 gcgattacca ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg
    56101 ttgctgaatc tacgccagac atcccgaagg caccgtggga gatttaacaa tcgtgccttc
    56161 aggttttcct atttgaggat gcccggcgcg tcagtatttt tttatttagt attataacgt
    56221 tataagaatt acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca
    56281 gggtcaggtt agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg
    56341 ccaggatctc aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt
    56401 atcgcttgat agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga
    56461 actcgccgag tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg
    56521 cagcagcaat gccctgctcg cccggactct gggcgagcag aagaaaaatg tcactatcat
    56581 cacggtcagc agctacatcg cgcatttgct gaaagacgcg ccttgtgaaa ttattttgct
    56641 cggtggcgtg taccagaaaa aaagcgaaag tatggttggc ccgttgacac gccagtgtat
    56701 ccaacaggta catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg
    56761 attcaccggt cgcgatatga tgcgtaccga tgtggtcaat gccgtgctgg aaaaagagtg
    56821 cgaagcgata gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg
    56881 tcccgttgag cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat
    56941 gcatcttgag cacagtaaac tcaccgttca cgttgttgac atttaaccat ttcccttctc
    57001 tacggatgat ttgcactttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta
    57061 agactattta cctgttatta acactctcaa gatataaaat tattatcagc gatataacag
    57121 gaagtcatta tcacctgcgt gatataaccc tgcgcgcgag cggatttcac ggaataattt
    57181 caccaggctt attcttagct attatagtta tagagagctt acttccgtga atcataaatt
    57241 caggagagag tattatgttt gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta
    57301 ccctggcaat gtctctgagt gcctgttcta actggtctaa acgggatcgc aacaccgcaa
    57361 tcggcgcggg tgcaggggca ttaggcggtg cagtactgac cgatggcagt acgttgggta
    57421 cattaggtgg cgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa
    57481 taaatgtcga ttatcgactc gcaattataa tacgcttgat tctgttcagt acgaaataac
    57541 tgaataatac acacagtaaa atattcgaaa gccacggata tatccgtggc tttttatgtt
    57601 aaccaccggc gagttttacc ttcatccctt tcgcttcaag cagtgatttt aataaatcac
    57661 gcttatcgcc ctggatttca ataattccat ctttaactgc cccgccgcag ccgcattttt
    57721 tcttcagttc cgctgccagt tttgtcagtt cggcatcatc gagatcgaca ccggtaatca
    57781 ggcaaacgcc cttaccttta cgtccactgg tctgacgctg aatacgcacc acaccgtcgc
    57841 ctttagggcg tacaggggcc gcttttggct catcaatacg tccggtttcc gttgagtaga
    57901 ccagacggct gttggaatca ctcatcatgc actccgctgt aaagaggcgt tgatcgcttt
    57961 cagcgtttgc gctggatcta ccgattgcgt taccgggcga ccaatcacca tataatcaac
    58021 accagccgcc aacgcctgtt ctggcgtcat aatgcggcgc tggtcaccag cttcactccc
    58081 ctgcggacga atgcccggcg taaccagttt gaactcctga ccaaatacct gtttaaagcg
    58141 cgcagcttcc tgagcagaac acaccacacc atcaaggcca catttttgcg tcagtgccgc
    58201 cagacgttct gcataatctg caggtgacag tgtcacgcca agatcggcca ggtcgctggc
    58261 ttccatgctg gtcaatactg tcacagcaat caaaagcggt gcatctttgc caaacggcac
    58321 cagtgcctca cgcgctgcgg tcatcatacg cgccccaccg gaggcgtgaa cattcaccat
    58381 ccacacgcct aagtcagctg cagcagcgac agcgtgcgct gcagtgttgg gaatatcgtg
    58441 aaatttcagg tcgaggaaga tatcaaaacc acgctgttga agttcgcgta caaattgagg
    58501 tccaaacagc gtaaacatct ctttgccgac tttcagacga caatcgcgtg ggtcgatctt
    58561 gtcgacaaag gccagcgcgg catcacgatt atgataatca agggcaacaa ccacaggaga
    58621 attcgtaaca gcgcgggaag aagatgaagc agttaacgtc atgaccagac cttcttgatg
    58681 atgggcaccg gaagtggcgc gaaacaggta aacggcgagc attctacccg tggaaataaa
    58741 aaattaacag ttgcgatttc tcccctggca ggcttttctg cgccggagtt accggagcac
    58801 aatgaacatc aattattagt atgttgtaac taaagtcgga tttttttaaa aattacaggc
    58861 catcaagacc gcgaatcggt ttaatggttg accaggcccg acaagacgga caatgccagt
    58921 agagggtgta tgcggtaaaa ccacatttct ggcagcgata acgaggctta ctacgcacct
    58981 tctcgccaac catgtcacgc agcaccatca gactctcttt ggcacgccct tcttccgctt
    59041 catttaagtg gtaatccatt agcttatgga acacacgcat ggtcggatga cgctgaagct
    59101 gacgcgtaat atagacctgt gcggcctcac taccgtcgcg cgcttcgata atatccgcaa
    59161 gcatcaattc agcatcggca ccggtgttct cttccaccgc gcgctgcagg aattctgccc
    59221 attcggcagt tttacccaac tgctgatagc aggtttgcaa catttccagc gtttcgctga
    59281 ccagttctct gtcctgagat atcacgcgtt gcagactttc gacggctttg gcgtattctc
    59341 ctttcgccat aaatacgcgt cccatcatga tggatacgcg ggcgctgttt ttatctgccg
    59401 ccgcaccttt ttttaacaag gccatggcgc gatcgagatc gtcgctggcc atatgctgca
    59461 gggctaactc acagtagaaa tgggcaattt cgacgcgctg cttatcttta cccaacttca
    59521 ccagacgttc ggcaacatca attgctttct gccactcgct ggtagcctgg tagatttgta
    59581 gcaactgttg cagcgcgcca atgcggaagt cagtttcatc ggtcagctga ttgaacatgt
    59641 cttccgcgcg gtcgtataac ccggcggcca tgtaatcacg ccccagttgt tgaatcgcca
    59701 acagacgctg ttcataggtc agcgaggcgc tttccattag ggtctgatgg atgcgaatag
    59761 cgcgatcaac ttcgccacgc gaacggaaca ggtttcctag cgtaaggtgg gcttcaacgg
    59821 tacccgtatc ctctttaagc atatcgagaa acaggtctac cgctttatcc tgttgattac
    59881 taagcaggaa gttaaccccc gctacgtaat cacgcgacaa gcggttggct tcatcttgct
    59941 tgttttgttg cgcacttctg cggcccatat accagccata ggcagcggct acaggcaaaa
    60001 gcagaaacaa caactccagc atagaaagtt attccttcgc cgctgacgaa tgcggcgcta
    60061 cagccacgtc agtcgcgggt gaaagctggt tttccagtcg ctttatttta cgttcagcgc
    60121 gcgccaggga aacacgaact cgcagccaga acagaccgca aatcagccaa ccgatagcaa
    60181 accccgcagc aaacaatacc gccagcaatg tggaaatacg gtactcccct tgcgctaaca
    60241 gataattaaa cgtcacctgt tgatcgttct gcgcacctaa cgtgaccgaa atcacgaaga
    60301 tcgctaacac cagtaagaaa atgagtaaat atttcacatt acttcccgtt atgtggttag
    60361 agcgaataaa ttatggtcaa cttaccgcaa tagcccttta aagtaccatt ttcgcgccgt
    60421 ctgcgaaatg gcatccacgc gcattgatga tgatttaagg gctaaattcg gaaaatcaac
    60481 cagcgttaac tttcttgttc tcgttgcgct atttcgcgat tttcttccgc aggtggtgtt
    60541 aatggcccac aaattcgttg cgcaagccac gttgccaccg ccaccagcgc ccacgaaatc
    60601 aacgtagcta ccaccagatc gcgtggccaa tgcatcccga gcagcaggcg gcttcccatg
    60661 actcccgttg cccagaccaa caagatagca atggttaacg ttcgccgacg cggccacagc
    60721 aaaccaacgg ccagcagcgc ccaactggca gcaaacatcg tgtgaccgga aggaaaggca
    60781 aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg ggatattttt ctcttcagcc
    60841 aactgttctt tcaccagatt tccgcgttct gctcgcttta aagtgtagaa ctcatcaacc
    60901 ggaatatgat gtgttttttc cagccagata acaaaaggtc gtggttcctg gactttgtct
    60961 ttgatccagg atttaacgcc ttgtcccaca agaattgccg ccgccagaat ggcaaataat
    61021 acaaaggcag ccttaatgcg aaaacgcaga caccagagaa accagccgaa taaaatcaaa
    61081 tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa cccaaaaagc cgcttttagt
    61141 agccaacttt gttctccagg ttgccaacgc caaccagaaa tccatacggc tactggcatg
    61201 acaagcaata gtgcagctcc cactgcggta cgtctggcaa tcgaacgcat ggcctctcct
    61261 ttttgataag tcccacaatc ataactgaaa acgccagttc caggaaaaat tgacagattt
    61321 gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga accccttctc gttatggcaa
    61381 aataagccaa tacagaacca gcattatctg gagaatttca tgcagcttaa acgtgtggca
    61441 gaagccaaac tgccaacccc atggggcgat ttcctgatgg tgggatttga agaactggca
    61501 accggacacg atcatgtcgc gctagtctat ggcgatatct ccgggcatac cccggtactt
    61561 gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt tcagcttgcg ctgcgattgt
    61621 ggcttccagc tcgaagcggc actgacgcaa attgccgaag aaggccgtgg tattttgctg
    61681 tatcaccgtc aggaaggtcg taacattggt ctgctgaata aaatccgcgc ttatgcactg
    61741 caggatcaag gttacgatac tgtagaggct aaccaccagt taggcttcgc cgctgatgag
    61801 cgcgacttca ctctttgcgc tgatatgttc aaactccttg gcgtcaatga agtccgcttg
    61861 ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag cagggattaa tattgttgaa
    61921 cgcgtaccat tgattgtagg tcgtaacccc aataacgaac attatctcga taccaaagcc
    61981 gagaaaatgg gccatttgct gaacaaataa ccctcttgca ttgtgtaatt catttgcttg
    62041 ccggaagcaa aataaccggc aagcaaatac ttgttacttc aacatattac gaatgacata
    62101 atgcaaaatg ccgtcgttct ggtagtaggt caactccgtc gcggtgtcga tacgacaacg
    62161 gcagggtacg acttcctggc taccatccgc gcgcgtaagc gtcaccggaa ccgtcgcgcc
    62221 gggttgtagg ttttgcagat cgccaatatc aatcttctct tccccggtta gccctaacgt
    62281 tttacgcgtt acgccttgcg gaaattccag cggcaggatg cccatgccaa ttaaattcga
    62341 acggtgaatt cgttcaaacg attcggcaat caccacacga ataccaagca gacgcggacc
    62401 ttttgccgcc cagtcacggc tggagcctga tccatactct ttcccggcaa tcaccgccag
    62461 cggcgtttgc tcctgcttat agcgcatcgc ggcatcatag atagagacat catcgctgtc
    62521 aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc atttcattac ggatgcgaat
    62581 attggcgaag gtgccgcgca tcatcacttc atggttacca cgccgcgaac cgtaggagtt
    62641 aaagtctttt cgctcaacac cccgaccttg tagatatcga cccgccgggc tgtcgggctt
    62701 aatactgccc gccggagaga tatggtcagt ggtgactgaa tcccccagca ttgcgaggat
    62761 ccgcgcaccg tgaatatctt ccacaggtgc tggtgttgcc tgcatttcat caaagaatgg
    62821 cgataagcga atataggttg aatcttcctg ccaaccgtag gtatcggatc gtgtgacgtt
    62881 aatttccttc cactctgctg tgccttcaaa aacttctgcg tactctttgc ggaacatttc
    62941 tgtggagact tgttctaccg cacgggcaat ttcttgtgcc gagggccaga tatctttcag
    63001 atataccggg tcgcctttac gatcgtgacc aataggctca gaagcgaggt taatattcat
    63061 atttcccgcc agcgcatagg caaccaccag cggcggcgag gccagccagt tagttttaac
    63121 cagcggatgg atacggcctt caaagttacg gttgccggac agcaccgcac cgacggttaa
    63181 atcgcctttt ttgattgccg tttcgatagg atcgggcagc ggcccagagt taccaataca
    63241 ggtggtacaa ccgtacccca caaggttaaa ccccagttcg tcgagatacg gtgtcaattt
    63301 cgcttttgcc agatagtcag aaacaacttt cgagcctggt gccagcgacg ctttgaccca
    63361 cggttgccgc ttgaggccca gagttacagc ttttttcgcc agcaagcctg cggccatcag
    63421 cacacttggg ttagaggtgt tggtgcacga ggttatcgca gcaatgacca ccgcgccatc
    63481 aggtaactga tactgatgtc cgttcataac ataatcgacc ggctggcgat ctttatgcgt
    63541 ggcattcact tccagttcgt tactggcggc aaatgctttt ggtacatcgg gcagtgcaac
    63601 gcgatcctgt gggcgtttag gccctgccag gctcgcttca acgtcattca tatccagttc
    63661 taacgtactg gtaaaaattg gttcatcgcc cgggttacgc cacatgccct gcgctttggc
    63721 atatttttcg accaactcga cctgatcttc gctgcgcccg cttaaacgca tgtaatcgag
    63781 ggtgacagca tcgattggga agaagccaca ggtggcacca tattctggcg acatattggc
    63841 aatggtggcg cgatccgcca gcggtagtga atccagccca tcaccataaa attcgacgaa
    63901 tttccccacc acgccatgtt tgcgcagcat ttgggtaacg gtgagaacca ggtctgtggc
    63961 ggtaatacct tcacgtaatt ttccggtgag tttaaagccc actacgtccg ggataagcat
    64021 ggaaaccggc tggcctaaca ttgcggcttc ggcttcgatg ccaccaacgc cccaccccag
    64081 cacgccaagg ccgttgatca tggtggtgtg cgagtcagta ccaacgagtg tatccggata
    64141 agcaatccat tcaccgtcct gcaattcgct ccacactgct ttgccgagat attcgaggtt
    64201 aacctgatgg caaatgcctg tgcctggcgg cacgacgcta aaacgactga acgcctgctt
    64261 tccccatttc aggaacacat aacgttcgtg gttgcgctcc atttccaggc gtacgttttc
    64321 ttcaaatgcc tcatcatcac caaaacgatc gacggtcacc gagtggtcaa tgaccaggtc
    64381 gaccggtgag agcgggttaa cctttgcagt atcgccaccg aggcgtttaa ccgcttcgcg
    64441 cattgccgcc agatcaacaa cggcaggtac gccggtaaag tcctgcatca gcacccttgc
    64501 cgggcggtag gcaatttcac ggtcagcatg ggcatttttc agccatcccg ccagcgcgtg
    64561 gatatcctct tcggtaaccg agttaccatc ctgccagcgc agcaggtttt cgagcaaaac
    64621 tttgagtgac ttaggcagac gggtgatatt gcccagtgat ttggcagcaa gcggcaggct
    64681 gtagtagtgg taagttttat ctttggcctg caacgtgtcc ttactggctt ctcgtagggt
    64741 tgacgacata gctcctcctt aatgacaggg ttgcgaagcc ctgattctct tcagggtgaa
    64801 tattaaagat aacacaaaca tcgcgtaacg atttgataac aacccaaatt gataaaagag
    64861 gagaaacctt gagacaaagc aaacagcggg gtcagggatc agtgagtaat gatccagagg
    64921 agttcggccc agaagagcat cgacagagaa aatgcgccca tccaggacca atatttcata
    64981 tttgtgttga tcataggcta cacctttaac ggttattact ttgtgttgag ataactcaac
    65041 ctgttattgc tgcggtgtat taaaagccct ttaattcatt aaaaatgggt aataagaatg
    65101 atgataatta ttgtttctgc tgatcatctt cagcaaacag ttctataaag gcttgctgct
    65161 gcgatgttag ttgccagtcg tctggaataa cgacggaaca ttcccgtttc gtttctgttt
    65221 cattgtcact atgctgacat aatggtgacg actccagttg ctcctgaccg accatagtta
    65281 tccccacact ttccaaatca gcagtgcgac aaccacccag aaaagagcag aaccaagaaa
    65341 taccgccagc caggctttgc gttttaattc agggtcccgg cggggttcct gatttccaga
    65401 aggcattgct aacctcatat aatcgccatc acttatcagc aagacgttga acgattggtg
    65461 taattaatca tacgatgaga caaatcctaa agaaacttta gtcaaaaatc cagcgaattt
    65521 acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt tgaaaactcg
    65581 agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat aaatatg
//
LOCUS       DAZKJR010000008        64282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKJR010000008 DAZKJR010000000
VERSION     DAZKJR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.008"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(175..996)
                     /gene="dapB"
                     /locus_tag="ACHOWS_000521"
     CDS             complement(175..996)
                     /gene="dapB"
                     /locus_tag="ACHOWS_000521"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0046162.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(1163..2077)
                     /gene="rihC"
                     /locus_tag="ACHOWS_000522"
     CDS             complement(1163..2077)
                     /gene="rihC"
                     /locus_tag="ACHOWS_000522"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFQ0046163.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(2143..3093)
                     /gene="ispH"
                     /locus_tag="ACHOWS_000523"
                     /gene_synonym="lytB"
     CDS             complement(2143..3093)
                     /gene="ispH"
                     /locus_tag="ACHOWS_000523"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFQ0046164.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKGVNCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3095..3544)
                     /gene="fkpB"
                     /locus_tag="ACHOWS_000524"
     CDS             complement(3095..3544)
                     /gene="fkpB"
                     /locus_tag="ACHOWS_000524"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0046165.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3652..4146)
                     /gene="lspA"
                     /locus_tag="ACHOWS_000525"
     CDS             complement(3652..4146)
                     /gene="lspA"
                     /locus_tag="ACHOWS_000525"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0046166.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4146..6962)
                     /gene="ileS"
                     /locus_tag="ACHOWS_000526"
     CDS             complement(4146..6962)
                     /gene="ileS"
                     /locus_tag="ACHOWS_000526"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0046167.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSLSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCANAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7005..7946)
                     /gene="ribF"
                     /locus_tag="ACHOWS_000527"
     CDS             complement(7005..7946)
                     /gene="ribF"
                     /locus_tag="ACHOWS_000527"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFQ0046168.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(7954..8172)
                     /gene="yaaY"
                     /locus_tag="ACHOWS_000528"
     CDS             complement(7954..8172)
                     /gene="yaaY"
                     /locus_tag="ACHOWS_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFQ0046169.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8275..8538
                     /gene="rpsT"
                     /locus_tag="ACHOWS_000529"
     CDS             8275..8538
                     /gene="rpsT"
                     /locus_tag="ACHOWS_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0046170.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(8634..9539)
                     /gene="nhaR"
                     /locus_tag="ACHOWS_000530"
     CDS             complement(8634..9539)
                     /gene="nhaR"
                     /locus_tag="ACHOWS_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFQ0046171.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(9599..10765)
                     /gene="nhaA"
                     /locus_tag="ACHOWS_000531"
     CDS             complement(9599..10765)
                     /gene="nhaA"
                     /locus_tag="ACHOWS_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFQ0046172.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     SVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            11351..11503
                     /gene="mokC"
                     /locus_tag="ACHOWS_000532"
     CDS             11351..11503
                     /gene="mokC"
                     /locus_tag="ACHOWS_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFQ0046173.1"
                     /translation="MKQPKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(11607..12737)
                     /gene="dnaJ"
                     /locus_tag="ACHOWS_000533"
     CDS             complement(11607..12737)
                     /gene="dnaJ"
                     /locus_tag="ACHOWS_000533"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0046174.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(12826..14742)
                     /gene="dnaK"
                     /locus_tag="ACHOWS_000534"
     CDS             complement(12826..14742)
                     /gene="dnaK"
                     /locus_tag="ACHOWS_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0046175.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEVGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            15118..15522
                     /gene="yaaI"
                     /locus_tag="ACHOWS_000535"
     CDS             15118..15522
                     /gene="yaaI"
                     /locus_tag="ACHOWS_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFQ0046176.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            15548..16261
                     /gene="msyB"
                     /locus_tag="ACHOWS_000536"
     CDS             15548..16261
                     /gene="msyB"
                     /locus_tag="ACHOWS_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFQ0046177.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            16410..16976
                     /gene="satP"
                     /locus_tag="ACHOWS_000537"
     CDS             16410..16976
                     /gene="satP"
                     /locus_tag="ACHOWS_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFQ0046178.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(17011..17598)
                     /gene="mog"
                     /locus_tag="ACHOWS_000538"
     CDS             complement(17011..17598)
                     /gene="mog"
                     /locus_tag="ACHOWS_000538"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFQ0046179.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYIETAPEVVAAFRPKSARRDVSE"
     gene            complement(17713..18666)
                     /gene="tal"
                     /locus_tag="ACHOWS_000539"
     CDS             complement(17713..18666)
                     /gene="tal"
                     /locus_tag="ACHOWS_000539"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFQ0046180.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            18945..20375
                     /gene="yaaJ"
                     /locus_tag="ACHOWS_000540"
     CDS             18945..20375
                     /gene="yaaJ"
                     /locus_tag="ACHOWS_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFQ0046181.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            20445..21221
                     /gene="yaaA"
                     /locus_tag="ACHOWS_000541"
     CDS             20445..21221
                     /gene="yaaA"
                     /locus_tag="ACHOWS_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFQ0046182.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(21261..21557)
                     /locus_tag="ACHOWS_000542"
     CDS             complement(21261..21557)
                     /locus_tag="ACHOWS_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFQ0046183.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEMTLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(21771..23057)
                     /gene="thrC"
                     /locus_tag="ACHOWS_000543"
     CDS             complement(21771..23057)
                     /gene="thrC"
                     /locus_tag="ACHOWS_000543"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFQ0046184.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(23058..23990)
                     /gene="thrB"
                     /locus_tag="ACHOWS_000544"
     CDS             complement(23058..23990)
                     /gene="thrB"
                     /locus_tag="ACHOWS_000544"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFQ0046185.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(23992..26454)
                     /gene="thrA"
                     /locus_tag="ACHOWS_000545"
     CDS             complement(23992..26454)
                     /gene="thrA"
                     /locus_tag="ACHOWS_000545"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFQ0046186.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(26480..26599)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(26535..26600)
                     /gene="thrL"
                     /locus_tag="ACHOWS_000546"
     CDS             complement(26535..26600)
                     /gene="thrL"
                     /locus_tag="ACHOWS_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFQ0046187.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(26814..27500)
                     /gene="yjtD"
                     /locus_tag="ACHOWS_000547"
     CDS             complement(26814..27500)
                     /gene="yjtD"
                     /locus_tag="ACHOWS_000547"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFQ0046188.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(27900..28040)
                     /gene="yjjY"
                     /locus_tag="ACHOWS_000548"
     CDS             complement(27900..28040)
                     /gene="yjjY"
                     /locus_tag="ACHOWS_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFQ0046189.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            28136..28852
                     /gene="arcA"
                     /locus_tag="ACHOWS_000549"
     CDS             28136..28852
                     /gene="arcA"
                     /locus_tag="ACHOWS_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFQ0046190.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(28911..30263)
                     /gene="creD"
                     /locus_tag="ACHOWS_000550"
     CDS             complement(28911..30263)
                     /gene="creD"
                     /locus_tag="ACHOWS_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFQ0046191.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFIAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(30321..31745)
                     /gene="creC"
                     /locus_tag="ACHOWS_000551"
     CDS             complement(30321..31745)
                     /gene="creC"
                     /locus_tag="ACHOWS_000551"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFQ0046192.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDTTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPTDPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(31745..32434)
                     /gene="creB"
                     /locus_tag="ACHOWS_000552"
     CDS             complement(31745..32434)
                     /gene="creB"
                     /locus_tag="ACHOWS_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFQ0046193.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLTLHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(32447..32920)
                     /gene="creA"
                     /locus_tag="ACHOWS_000553"
     CDS             complement(32447..32920)
                     /gene="creA"
                     /locus_tag="ACHOWS_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFQ0046194.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            33131..34000
                     /gene="robA"
                     /locus_tag="ACHOWS_000554"
     CDS             33131..34000
                     /gene="robA"
                     /locus_tag="ACHOWS_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFQ0046195.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(33997..34644)
                     /gene="gpmB"
                     /locus_tag="ACHOWS_000555"
     CDS             complement(33997..34644)
                     /gene="gpmB"
                     /locus_tag="ACHOWS_000555"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFQ0046196.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            34696..35211
                     /gene="yjjX"
                     /locus_tag="ACHOWS_000556"
     CDS             34696..35211
                     /gene="yjjX"
                     /locus_tag="ACHOWS_000556"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFQ0046197.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            complement(35205..35531)
                     /gene="trpR"
                     /locus_tag="ACHOWS_000557"
     CDS             complement(35205..35531)
                     /gene="trpR"
                     /locus_tag="ACHOWS_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFQ0046198.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(35621..37558)
                     /gene="sltY"
                     /locus_tag="ACHOWS_000558"
     CDS             complement(35621..37558)
                     /gene="sltY"
                     /locus_tag="ACHOWS_000558"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFQ0046199.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWELSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            37894..39561
                     /gene="ettA"
                     /locus_tag="ACHOWS_000559"
     CDS             37894..39561
                     /gene="ettA"
                     /locus_tag="ACHOWS_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFQ0046200.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(39617..39901)
                     /gene="nadS"
                     /locus_tag="ACHOWS_000560"
     CDS             complement(39617..39901)
                     /gene="nadS"
                     /locus_tag="ACHOWS_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFQ0046201.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPDFFRELASH"
     gene            complement(39903..40235)
                     /locus_tag="ACHOWS_000561"
     CDS             complement(39903..40235)
                     /locus_tag="ACHOWS_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0046202.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(40327..41559)
                     /gene="nadR"
                     /locus_tag="ACHOWS_000562"
     CDS             complement(40327..41559)
                     /gene="nadR"
                     /locus_tag="ACHOWS_000562"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFQ0046203.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(41580..42962)
                     /gene="radA"
                     /locus_tag="ACHOWS_000563"
     CDS             complement(41580..42962)
                     /gene="radA"
                     /locus_tag="ACHOWS_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0046204.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(43011..43979)
                     /gene="serB"
                     /locus_tag="ACHOWS_000564"
     CDS             complement(43011..43979)
                     /gene="serB"
                     /locus_tag="ACHOWS_000564"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFQ0046205.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            44085..44729
                     /gene="ytjB"
                     /locus_tag="ACHOWS_000565"
     CDS             44085..44729
                     /gene="ytjB"
                     /locus_tag="ACHOWS_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFQ0046206.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            44757..45773
                     /gene="lplA"
                     /locus_tag="ACHOWS_000566"
     CDS             44757..45773
                     /gene="lplA"
                     /locus_tag="ACHOWS_000566"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFQ0046207.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            45805..46068
                     /locus_tag="ACHOWS_000567"
     CDS             45805..46068
                     /locus_tag="ACHOWS_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0046208.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(46229..46948)
                     /gene="deoD"
                     /locus_tag="ACHOWS_000568"
     CDS             complement(46229..46948)
                     /gene="deoD"
                     /locus_tag="ACHOWS_000568"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0046209.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(47005..48228)
                     /gene="deoB"
                     /locus_tag="ACHOWS_000569"
     CDS             complement(47005..48228)
                     /gene="deoB"
                     /locus_tag="ACHOWS_000569"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0046210.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(48280..49602)
                     /gene="deoA"
                     /locus_tag="ACHOWS_000570"
     CDS             complement(48280..49602)
                     /gene="deoA"
                     /locus_tag="ACHOWS_000570"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFQ0046211.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(49680..50459)
                     /gene="deoC"
                     /locus_tag="ACHOWS_000571"
     CDS             complement(49680..50459)
                     /gene="deoC"
                     /locus_tag="ACHOWS_000571"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0046212.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            50717..52267
                     /gene="yjjI"
                     /locus_tag="ACHOWS_000572"
     CDS             50717..52267
                     /gene="yjjI"
                     /locus_tag="ACHOWS_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFQ0046213.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            52239..53102
                     /gene="yjjW"
                     /locus_tag="ACHOWS_000573"
     CDS             52239..53102
                     /gene="yjjW"
                     /locus_tag="ACHOWS_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFQ0046214.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(53320..54099)
                     /gene="yjjV"
                     /locus_tag="ACHOWS_000574"
     CDS             complement(53320..54099)
                     /gene="yjjV"
                     /locus_tag="ACHOWS_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0046215.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLKKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYTLFNVP"
     gene            complement(54096..55169)
                     /gene="yjjU"
                     /locus_tag="ACHOWS_000575"
     CDS             complement(54096..55169)
                     /gene="yjjU"
                     /locus_tag="ACHOWS_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFQ0046216.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(55291..55452)
                     /gene="ytjA"
                     /locus_tag="ACHOWS_000576"
     CDS             complement(55291..55452)
                     /gene="ytjA"
                     /locus_tag="ACHOWS_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFQ0046217.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(55579..56184)
                     /gene="osmY"
                     /locus_tag="ACHOWS_000577"
     CDS             complement(55579..56184)
                     /gene="osmY"
                     /locus_tag="ACHOWS_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFQ0046218.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(56577..58166)
                     /gene="prfC"
                     /locus_tag="ACHOWS_000578"
     CDS             complement(56577..58166)
                     /gene="prfC"
                     /locus_tag="ACHOWS_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0046219.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQSAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(58257..58934)
                     /gene="yjjG"
                     /locus_tag="ACHOWS_000579"
     CDS             complement(58257..58934)
                     /gene="yjjG"
                     /locus_tag="ACHOWS_000579"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFQ0046220.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(58949..59395)
                     /gene="rimI"
                     /locus_tag="ACHOWS_000580"
     CDS             complement(58949..59395)
                     /gene="rimI"
                     /locus_tag="ACHOWS_000580"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0046221.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(59364..59777)
                     /gene="holD"
                     /locus_tag="ACHOWS_000581"
     CDS             complement(59364..59777)
                     /gene="holD"
                     /locus_tag="ACHOWS_000581"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFQ0046222.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            59880..60911
                     /gene="rsmC"
                     /locus_tag="ACHOWS_000582"
     CDS             59880..60911
                     /gene="rsmC"
                     /locus_tag="ACHOWS_000582"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFQ0046223.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            61111..61197
                     /locus_tag="ACHOWS_000583"
     tRNA            61111..61197
                     /locus_tag="ACHOWS_000583"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61145..61147,aa:Leu,seq:cag)
     gene            61226..61312
                     /locus_tag="ACHOWS_000584"
     tRNA            61226..61312
                     /locus_tag="ACHOWS_000584"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61260..61262,aa:Leu,seq:cag)
     gene            61347..61433
                     /locus_tag="ACHOWS_000585"
     tRNA            61347..61433
                     /locus_tag="ACHOWS_000585"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61381..61383,aa:Leu,seq:cag)
     gene            complement(61472..61708)
                     /gene="yjjZ"
                     /locus_tag="ACHOWS_000586"
     CDS             complement(61472..61708)
                     /gene="yjjZ"
                     /locus_tag="ACHOWS_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFQ0046224.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     AMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
     gene            61849..62637
                     /gene="fhuF"
                     /locus_tag="ACHOWS_000587"
     CDS             61849..62637
                     /gene="fhuF"
                     /locus_tag="ACHOWS_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFQ0046225.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSQLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(62674..63351)
                     /gene="bglJ"
                     /locus_tag="ACHOWS_000588"
     CDS             complement(62674..63351)
                     /gene="bglJ"
                     /locus_tag="ACHOWS_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFQ0046226.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(63309..64034)
                     /gene="yjjQ"
                     /locus_tag="ACHOWS_000589"
     CDS             complement(63309..64034)
                     /gene="yjjQ"
                     /locus_tag="ACHOWS_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0046227.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        15417 a        17408 c        15963 g        15494 t
ORIGIN      
        1 caaatggtca aaatttacat taaaacaaca aaaacagaat acaaaacatg taattttcag
       61 cacttgcatt aaatttaaaa gcataacacc aaaaataaaa gggctaaaaa ttaaagccct
      121 ttatattcaa tgcattaaat gtgttatttt tgcatcacta taaatatttt gtggttacag
      181 attattgaga tcaagcacat ctcgcatatc aaaaagacca ctttccttac cactcaacca
      241 caaagccgat cttaccgcac cgttagcaaa tgtcatgcgg ctggacgcct tatgggtgat
      301 ctccagacgc tcgccaatat cggcaaacat cgcggtatgt tcaccaacga tgtcacctgc
      361 acgcacggtg gcaaaaccaa tggtgccagg cacacgttca ccggtatggc cttcacgact
      421 gtagaccgcg caatctttca gatctttatc cagagcgtgg gcgatcgcct ctcccattgc
      481 cagtgcggtg cctgacggcg catcaacttt atgtctatga tgtgcttcaa taatttcgat
      541 atcggtgtag tcacccatca ctttggctgc tttctccagc agtttaagca tgacgttaac
      601 gccaacgcta aaattggcgg caaagacaat cgcaatatcg gcggcggcgt cacgaatcgc
      661 ctgtttaccg gcttcgtcaa accccgtagt gccgatcacc atccctttgc catgctggcg
      721 acaaaaagcg agatggttca gcgtaccttc cggacgggta aaatcgataa acacatcaaa
      781 atcatctttt atcgcatcaa gactactttg cacagtaacg cctgttttcc cggctccggc
      841 cagttcgccc gcatcgctgc ccagtaaaga agatccttcg cgctccagcg ccgcgcccag
      901 ctgcacgcct tctaatgcca gcgccgcctg aatcaactgg cggcccatac ggcccccggc
      961 tcccgcgatg gcaacgcgga tgtttgcatc atgcatagct attctctttt gttaatttgc
     1021 atagaccgtt ttcagagtaa ccagcgcatc gctacgccgc cagccaaaaa cgctgataat
     1081 tagaaattaa tgataaacag ggagggatat cagtgaaagg catgagtcga tgaatgactg
     1141 catgccgata acatgtgaca ggttacggca ccagagccag cacctcagct acccactgct
     1201 ggaagccttt tacattcaga tccaatgcca cctgtacatt ggctggtttg cccaggcaac
     1261 cgtcgatatc aaccaccgtc gtgccagagg taaattcgcc ctgcgtttcc actgccacaa
     1321 aacacggctt gagagtgaac aggtccgggc gcaccagcca ggcgatggcg cagagatcgt
     1381 gcattcgcag tccactttgc atactgccgc tacggtagtg actaaacagg gcgtggagca
     1441 tttttccggt gcgattcaac tccggcaagg tagcgagata gtcaggggtt aatattgcct
     1501 gattggtgac atccagaccg cacatgacga tttcgatgcc gctacggaag acacaggctg
     1561 ccgcttctgg atcggcagcg atattaaact cggcgtttgg tgtgcagttg ccgcgaccag
     1621 cagaaccgcc catgatcacc agacggcgaa tatacggctt acattccggg cattgtgaaa
     1681 gtaacagcgc aatattggtt aacgggccga tggccaccag ggtaacgggc tctggcgcac
     1741 gcatcagggc atcacgaatt gccagaaacg ccggtatacc cagtggctga cggttgtgtt
     1801 caacaaagtc atagccagcc attcccgatt caccgtgtac agatgccgca tcgcgcggcg
     1861 cacgaaccag tggtacggca gcgccctggg caagcggaat ctccgcattc cagaaatgca
     1921 gcagttgcag ggcgttgcgg gtcgttttct ctaccgaaac attacccgcg acagtggtca
     1981 tcagttgcag atcgagttcg ggggtaaaaa tcgcggcggc aatggcaacg gcatcgtcaa
     2041 tgccggggtc tgtatcgagg aagataggta aacgcatgtt ttctccataa aaaatgccgg
     2101 taacaagacc ggcattttcg cataacttag gctgctaatg acttaatcga cttcacgaat
     2161 atcgacacgc agctctttcg gcacttcgaa aacaatgttt tcttcacgac cttccagcgg
     2221 aatggcttca ccaccgccca gctgttgcaa acgtgccacc acattctgca ccagaatatc
     2281 cggagccgat gcgcccgcag tcacgccaac gcagttcacg ccttttaccc actcttcctg
     2341 gatatctttc gcatcgtcaa tcaaaaatgc gcgtttgccc atacgctggg ccagttccgc
     2401 cagacggttg gagttggagg agtttttcga accaaccacc agtactactt ccgcctgctc
     2461 tgccagggcg cgtaccgcct cctgacggtt agtcgtggcg tagcagatgt catctttgcg
     2521 cggaccgaca attttcggga agcgtttacg cagcgcgtcg atcacatcag acgtgtcatc
     2581 caccgacagc gtggtttggg tcataaagga gagcttctct tcgtttttga ccgtcagttt
     2641 ccacacatcg tctggcgatt cgaccagata cattccccct tccgggttgc tgtactggcc
     2701 catcgtccct tccacttccg ggtgcccggc gtgaccgatg agaatagatt cttcaccacg
     2761 acggctggcg cgggcgactt ccatatgcac tttggtcacc agcggacagg tggcatcaaa
     2821 caccgtcaaa tcgcggcttt tcgcttcgtt acgtactgcc tgagaaacac cgtgtgcgga
     2881 gaaaatcagg atcgcgccgt ccggtacttc gctaatctgc tcaataaaga tagccccacg
     2941 ctcgcgcaag ctatcgacca cgtagcggtt atgcaccact tcgtgacgga catatatcgg
     3001 tgcgccgtaa atggccagcg cgttttcaac aatgctgata gcgcggtcta ccccggcaca
     3061 aaaaccacgc gggttggcca acaggatctg catgttacgc ctccagtgcc ggatcgattt
     3121 ccagcacttc aatatcaaaa tgaacggtct gcccggccag cggatggttg aaatcaacgg
     3181 taatggagtc gccgttaatt tcgcggatca cgccaggcat ctcactgcca tccattgcgg
     3241 taaaaagcat gattgcgcca atttctggct cgcctgcatc cataaattca cggcgggaga
     3301 agtactgaat caggtccggt gacggcacgc caaacgccgc atcaggctcc agcgagaagg
     3361 tggttttatc gcccactttc agccccagca ggtgttgctc cagcccttca gaaagagaag
     3421 catcacccag gcggaacagc gccggtttac cgttgttgcg ggtagactca gcggtggtgc
     3481 catcatcgag tttcagcgtg aagtgcacca ggacggcgct attgctctgt acagattcag
     3541 acatgcaggt ttgctcttat attttgagga tttttgccgg atgcggccta cgatttcgca
     3601 gcaatctgta ggccggataa gatgcgtcag catcgcatcc ggcagggttt attattgttt
     3661 tttcgcttta gaaggtaaaa aaccttccag cacaatcagt gccgcaccga cacaaatggc
     3721 agtatcggca aggttgaagg tggcgaagtg ccagtcgccg acatagaagt cgatcatatc
     3781 gacaacgaag ccgtgccaca ggcggtcgaa caggttgccc agcgcgccgc caataatcag
     3841 cgcgtaagcg atattgttta gcttctgcgt ggccttcgag cgatacatca tcactgccag
     3901 gatcacgcta ataccaatcg caataccggc aaaaaaccaa cgctgccagc cgccactatc
     3961 ggcaaggaaa ctaaacgccg cgccatagtt acgcgcataa tgcagattaa gcgacgggaa
     4021 cagcgggacc gtatccccca gagcaaagtt ctggaggatc aggtatttgc tgcccagatc
     4081 gataatcagc acgactacca ccagccacag ccagcgtagc cctgttgaac agatcgattg
     4141 actcatcagg caaacttacg tttttcaccg tcaccggcga cgttgctgac acaacggccg
     4201 cagatttctg cgtgttccgc caccttgccg acatcctggg tgtagtgcca gcagcgtggg
     4261 cacttctcac cttcggcttt actcaacgcg actttcagcc ctttgaggac ttcgctctgc
     4321 tgggcgtcag caggtgcgtc gttatagtct gcaacggtag cgccggaggt caacaggaca
     4381 aatcgtaatt catcgcccag cgcggtcagt ttcgccgcca gttccggttc tgcatacaag
     4441 gttactgccg cttccagcga gccgcccact ttcttatcgg cacgcgcttg ctcaatgact
     4501 ttgttcactt cgccacgcac tttcaacagc tcgtcccaga acgcatcgtt cattgcttca
     4561 ctgtctgcca gaccaaacag gccttcgtac cactcaccgg tgaagacgta tttttcacgt
     4621 tcgcccggca ggtaacccca cacttcatca gcggtgaagg agaggattgg tgccatccag
     4681 cgaaccagcg cttctgcgat gtgatacagc gcagtctggc agctacgacg cgccacgctg
     4741 tccgctttgg cggtatactg acggtctttg atgatgtcga ggtagaagga ccccatctca
     4801 acggagcaga agcgcatcag acgctgtacc acttcgtgga aatcgtatgc ttcgtacgct
     4861 ttgaggatgt cttcctgtgc cgcgttcgca caacctacgg cccagcgatc cagtaccacc
     4921 atctcttccg gtttcaccat atcttttgcc ggatcaaaac cgttcaggtt tgccagcagg
     4981 aagcgcgcgg tgttacggat acgacgatag ctgtcggcag cacgtttcag gatctcgtca
     5041 gaaacggcca tttcgccagt gtagtcggtt gatgccaccc acagacgcag aatatccgca
     5101 cccagtttgt tcatcacatc ctgcggcgaa acggtgttac cgatggattt agacatcttg
     5161 cgaccctgac catccacggt aaagccgtga gtcagtacct gacgatatgg cgctttgccc
     5221 ttcatcgcgg tagagatcat cagggaagac atgaaccagc cacggtgttg gtcagaacct
     5281 tccagataca tgtccgctgc gtgaccggca aattccggac gcacatcaac aacggaagag
     5341 tgggtagatc cggagtcaaa ccatacatcc agcgtatccg gtactttcac atactggtca
     5401 gcttcgtcgc cgaggatctc tttcgcatcg agatcccacc acgcctgaat gccgtcaact
     5461 tcaacgcgtt ttgccacttc ttccatcagt tcgagagtac gcggatgcag ttcttctgtg
     5521 tctttgtgca cgaacagtga catcggcacg ccccaggtac gctgacgaga gatacaccag
     5581 tcaggacggt tagcaaccat cgactcgata cgcgcctggc cccagtcagg gatccactgc
     5641 acgcctttga tctctttcag tgactgcgca cgcagacctt tctgatccat gctgacgaac
     5701 cactgcggcg tcgcgcggaa gatgatcggc gttttatgac gccagcagca cggatagctg
     5761 tgttgcattt tctcaacgtg caacagtgcg cctttttcct gcaacaacgc aatgacaata
     5821 tcgttcgctt tgaagacgtt aacgccatcc agagtcgggt aagtacccgg cagataagtg
     5881 ccgtccgggc caaccgggtt agcggtttcc agaccatatt tttgaccgat cacatagtcg
     5941 tccggaccgt ggcctggcgc ggtatgaacg gcaccggtac cggcatccag cgtaacgtgg
     6001 tcgccgagaa ttgccggaac atcgaagtcc ataaacggat gggtaaagcg caacagttcc
     6061 agctcggcac ctttcaccgt gccaagaatg gtgtaatcgc taacgccgat acgctgcatt
     6121 acgctttcaa ccagatcttt cgcgaggatc acggcctgac cgtcgatttg caccagcgca
     6181 taatcaaaat caggtgcgat ggagattgcg cggttagcag gcagcgtcca cggcgtggtg
     6241 gtccatataa ccagcgaaat tgggccgtta acattgctta cgccaaattt cgttttaagc
     6301 gcatcctgat cgaccgcctg gaaagcgacg tcgatggaca gagaagtttt gtcgtaatac
     6361 tcaacttccg cttctgccag tgcagaacgg cagtcaacgc accagtgcac cggcttcgcg
     6421 cctttgtgca ggtgaccgtt gccgatgatt ttgcccagcg cgcggatgat gttggcttca
     6481 gttttgaagt ccatggtcag gtacgggtgc gaccagtcgc ccagcacgcc cagacggata
     6541 aagtctttgc gctgaccgtc aacctgggtc gcagcgtatt cgcggcactt ggcgcggaac
     6601 tcagcggcgg tgaatttctc ccccggctta ccgtattctt gctctacttt cagctcgatt
     6661 ggcagaccat gacagtccca gccaggcaca tacggcgagt catatccaga aagccctttg
     6721 gacttaatga taatgtcttt cagaatcttg ttaaccgagt gaccaatatg aatactgcca
     6781 ttcgcataag gagggccatc atgcagaatg aaggtttttt tgcctttttt agctgcacga
     6841 atgatgccgt acagatcatc atcagtccaa cgcgccagca ttcccggttc gcgcttggcg
     6901 agatcgccac gcatcgggaa ccctgtttcc ggcaaattca gggttgattt atagtcactc
     6961 atcagattct cggttccgta tttcggtttg attacataac aggcttaagc cggttttgtt
     7021 agcccaaaaa attcgcgggc ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc
     7081 agcgatgcaa atcgctgctc attgcgtatt tttttacgca gcactacttg tatatggcga
     7141 ccataaaggt ccattgcaac atctaacaaa tgcacttcca gttgctggcg aataccggca
     7201 accgttgggc gtgttccgat gtttgcaacg ccgggtaacg gcttttcgcc aaggcccaac
     7261 acttctaccg cataaacccc tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc
     7321 gtcgggaaac ctatagtgcg ccctaattca tcaccgtgga ctacacgccc ggagatagca
     7381 aacgggtgcc ccagtaaact ttctgccaga gccagattgt catccgcaag cgcctgacgc
     7441 acggcggtgc tgctgatacg cacgccacct tcgcaaaaag tttgcgtgct ggtgatatcg
     7501 aagccgtatt ccatgcccgc tttctgtaat aacaagaaat cgccttcacg accagcgcca
     7561 aagcggaaat cgtcacctac ggcaagaaat tttacccgca agtgcttcac cagaagatca
     7621 ctgatgaagt tttgcgcggt taacgccgca aaacgcctgt caaaacgcac gcacagcacg
     7681 taatcaacac cacactctgc aagataacgc agtttttccc gcagccgggt gagccgtgcc
     7741 ggggctttat cagtagcaaa cagttccagt ggttgaggtt caaaaagcat caccatcacc
     7801 ggtaagttgc gcttgcgccc ttcttcctgc aagccctgta acagcgcgcg atgaccgcga
     7861 tgcacgccgt cgaaattacc aatagtcagc acacaccctt cttgcggggc ctggctgaga
     7921 ttatgtatgc cgcgtatcag cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat
     7981 accttgtaca gcggtcaagg ttaaccggcg attgagtacc gaaatcagaa agaagtggct
     8041 tttcattgct atggcgcaaa tcatgggaag aaactgaccg cctgctgcaa tttttctcgc
     8101 ggaaaagctg tattcacacc ccgcaagctg gtagaatcct gcgccatcac tacgtaacga
     8161 gtgccggcac attaacggcg cttatttgca caaatccatt gacaaaagaa ggctaaaagg
     8221 gcatattcct cggcctttga attgtccata tagaacacat ttgggagttg gaccttggct
     8281 aatatcaaat cagctaagaa gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca
     8341 agccgtcgct ctatgatgcg tactttcatc aagaaagtat acgcagctat cgaagctggc
     8401 gacaaagctg ctgcacagaa agcatttaac gaaatgcaac caatcgtgga ccgtcaggct
     8461 gctaaaggtc tgatccacaa aaacaaagct gcacgtcata aggctaacct gactgcacag
     8521 atcaacaaac tggcttaatt gcctgttgtt gtcagctttg taaaaaaacc cgctctggcg
     8581 ggttttttta tatccatctg caggccaaaa ttcggcctgc aaagtacaaa tgtttatttt
     8641 gaagctggag taaacagcgc agaatagtct gtattgcaga tacgctgtac tgcagggtgc
     8701 tgaatcatcc tttcggcaaa aatcgcgtgg tactcttcca tcacgttctc aacacggcct
     8761 atctccacaa ccgagtcatc gttatagaaa tcattagcgt aaagcgaagg tgcaacgaaa
     8821 atagcgttat gcgtcgcccc aaaggctttc atcaacgcag catcatcaaa ctcacccaaa
     8881 atttcgacgt tcaagccctg ggagttaaac cagtttaata gcttacgccc caacattgag
     8941 cgacgccccg gaataagtaa acgacgctct tcaagacagg caggaaacgg cttttctggt
     9001 agtgggttag tgcaccagaa actgacacca cattcgccaa ttttcatgga aaacagccct
     9061 tcctgctgag tggaatcgat cggacagtca gagatgatca tatccagttt atgctgactc
     9121 aactgctcta aaagcatctc gtgcgtcgat tcgaaacagc gtagatggat ctgctcgtct
     9181 tccacaactg cggcatccag aacactgctg accagacgtt tggaaagtgc atctgccaca
     9241 ccaacatcaa acaataagtt ggactctttg cgatagttga cgatatccag catttcctgg
     9301 cttaaggtga acattttatc ggcatagcga tagaccagct ctcccagctc gctgggctcg
     9361 aggccacgtc ccttgcgctt aaataatttg ccttgcaggc gctcttccag cgcccgaatc
     9421 tgtccggtaa tggtttgtgg tgttaaataa agcgcctccg ctgcgccaac cacggaacct
     9481 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     9541 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     9601 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     9661 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     9721 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     9781 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     9841 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     9901 gcagaatgga ggtcaagcca tccagcgtga cgccttgcag tgaaacgcca gcattagcaa
     9961 atgcaaacag cggtaaaatc agatacgcca cccacgggtg caacacatgc tccagtcgct
    10021 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
    10081 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacaccacg ccaaccagaa
    10141 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
    10201 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
    10261 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agggctaacg
    10321 gaacacgact tcccaacagc gccagcacac caagtgcaaa agcaatgtca gtagccgccg
    10381 ggatcgccca cccttcgcgg gtaatcggat cggcatggtt aaaggccaga tagagtaatg
    10441 ccggcacaat catcccacca atagcggcga taactggaaa tgcagcctgg cgtaagctgg
    10501 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
    10561 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
    10621 ggagctgaac cgacgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
    10681 ccataatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
    10741 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
    10801 actatcttac cccgcacgaa tcatcgttaa aatcagatta tagtgtagga ataattcgct
    10861 ttttacgagg cattgaagga tttgcgtcat cagtgatgag atgagttttt gacggctgta
    10921 agcgctgacg tgtgacccta agccctgata atcatagctt aaggtcagga tttctgaggg
    10981 attaattagg aaaagtaata attaatttgt tgtcagtcgg cgatgtttgt taggccaata
    11041 cttcaaaaat aatccagttt gtcgtcaata attgcgatga agagataaat taaagttaaa
    11101 taattaaatc attaaaaaat tacgccataa aaatgcgagg cgtcttcagg attagaatcg
    11161 ataaaacaga atgggttaaa gagagcgata taatagcggc gggtgcttga ggctgtctgt
    11221 ctcaggcatt agctgaacgg cagatagaga aaagccccga gtgatatttt actatcaacc
    11281 cgaggccccc tatatgcaga acacatgtag agtgcctctt actgaccgta aggtcaagga
    11341 gaagagagca atgaagcagc ctaaggcgat gattgtcgcc ctgatcgtca tctgtatcac
    11401 cgccgtagtg gcggcgctgg taacgagaaa agacctctgt gaggttcaca tccgaactgg
    11461 ccagacggag gttgctgttt tcacggctta cgaatccgag taagagcaac ggcggggagt
    11521 gatccccgcc acttttcagg tgctcgcata tcttcaacgc accctatttt tacccaggcc
    11581 tgcccacggg caggcttttg gggagattag cgggtcaggt cgtcaaaaaa cttcttcaca
    11641 ccatcaaaga agctctttga gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt
    11701 tcttgcagct cttgcagcag ctgtttctgc ttctcgttca ggcctaccgg tgtttcgacg
    11761 acaacgcggc acagcaaatc accctgtgcg ccaccgcgga cagacttgac gcctttaccg
    11821 cgcatacgga acagcttacc ggtctgggtt tcgccaggca ctttcagttt gacgcgacca
    11881 tcaagggtcg gtacttcgat ttcgccaccc agcgccgcca tagcgaagtt gatcgggact
    11941 tcgcaataga ggttgttgcc ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga
    12001 acgtacagat cgcctgccgg tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg
    12061 atgcggtctc cagtgtccac ccctgccggg attttaacgg acagcgtttt gctgcgctca
    12121 acacgaccat gaccatgaca tttgttgcac ggatctttga tcagcgtacc gcggccctga
    12181 cagtgtggac aggtctgctg cacggcaaag aaaccctggc gcatctgcac ctgaccagaa
    12241 ccatgacagg tcggacaggt ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa
    12301 acgtcacact cttccagagt cggaatgcgg atctctttgg tcacgccacg tacagcttct
    12361 tcgagggtga gctccatgtt atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca
    12421 cgtccgccgc caaaaatatc gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg
    12481 ccgccgaaac cgccgcctcc catgccacct tgctcaaacg cagcatgacc atactgatcg
    12541 tatgccgcac gtttttgcga gtcggtcaga acttcataag cttccttgat ctctttaaat
    12601 ttcgcctcgg cctctttgtc accctggtta cggtccgggt ggtatttcat ggccaggcgt
    12661 ttgtaggcct ttttgatttc acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa
    12721 taatcttgct tagccatctt ttttaaattg cccctagatg aatgcacggg cggagaggaa
    12781 attccccttc gcccgtgtca gtataattac ccgtttatag ggcgattatt ttttgtcttt
    12841 gacttcttca aattcagcgt cgacaacatc gtcatctttc gcgttgtttg cagaagcatc
    12901 agcaccggca gtctgctgct gggcatgttg ctgctgggcg atttccatca gtttctggga
    12961 aacctgtgcc agttcctgca ttttcgcttc gatagcggct ttgtcttcac ctttcagagc
    13021 agtttccagt gcagtcagtg cagactcgat agcagttttg tcgtcagccg gcagtttgtc
    13081 gcccacttct tcaacctgct tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt
    13141 ctgtaccagc tcttcaaact tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat
    13201 tttctggatt tcatcttcgt tcagaccaga agaagccttg atggtgatct tctgctcttt
    13261 accgctgttt ttatctttcg cggaaacgtg caggataccg tcagcatcga tatcgaaggt
    13321 aacttcgatc tgcggcatgc cgcgcggtgc cgggttgata ccatccaggt tgaactgacc
    13381 cagagatttg ttatcagctg cacgtttacg ttcaccctgc agcacatgga tggttaccgc
    13441 agactggttg tcttcagcgg tagagaacac ctggctgtgc ttggtcggga tagtggtgtt
    13501 tttcgcgatc agcgtcgtca tcacaccgcc catggtttcg atacccagag acagcggggt
    13561 aacgtccagc agcagtacgt cttttacgtc accagtcaga acaccaccct gaacagcagc
    13621 accgattgct acagcttcgt ccgggttaac gtctttacgc ggctctttac caaagaactc
    13681 agcaactttc ttctgaacca ttggcatacg agtctgacca ccaacgagga taacgtcgtc
    13741 gatatcagat acggacaggc cagcgtcctg cagtgcaact ttcagcggct caatggaacg
    13801 gtttaccaga tcttcaacca ggctttccag tttcgcacga gtcactttga tgttcatgtg
    13861 tttcggaccg gtcgcgtctg cagtgatgta cggcaggtta acgtcggtct gctgagcgga
    13921 agacagttcg attttcgctt tttctgccgc ttctttcagg cgctgcattg ccagcggatc
    13981 gttgcgcagg tcaatgccct gatctttctt gaattcttca accaggtagt tgatcagacg
    14041 gctgtcgaag tcttcaccac ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt
    14101 tttttcgccg tcaacttcgt cgatttcgat aatagaaata tcgaaagtac caccacccag
    14161 gtcataaacc gcgatagtac ggttgccagt acctttgtcc agaccgtaag ccagcgcagc
    14221 tgcggtcggt tcgttgatga tacgttttac ttccagacca gcgatacggc ctgcgtcttt
    14281 ggttgcctga cgctgagcat cgttaaagta tgccggtacg gtgataacag cttcagttac
    14341 cggttcaccc aggtaatctt cagcggtttt cttcattttt ttcagcactt cagcagagat
    14401 ctgcggcggt gccattttct ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc
    14461 aataattttg aacggcatga tggaaacatc acgctgtact tcttcgtcct ggaagcggcg
    14521 gccaatcagg cgtttaatcg caaacagggt gttttgcggg ttcgtcactg cctgacgttt
    14581 agccggctga ccaaccagag tttcaccatc ctgggtatag gcaatgatag aaggcgtggt
    14641 gcgatcgcct tcggcgttct ccagtacacg aggagtggtg ccatccataa tcgctacaca
    14701 agagttggta gtacccaggt cgataccaat tattttaccc atctaaacgt ctccactata
    14761 tattcggtca tcatgtggtt gtgagtctgt aataggggcg aaacgtctgg tttcaactgc
    14821 ccaatttcat ttttttgcag actcactcac tgcggttgac tactaaatgg ggtcgtaacc
    14881 cacgtcatca agggggagat gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata
    14941 tgccagaaca agacatgaaa ctgacagcaa ttttattcat atagagaatg aatagcagac
    15001 aagacattca tagaaaaatt gtaataaaaa gaaaatatta cgtacctgaa tcttctcttt
    15061 atcagcagta aactagtggg tattcatccc ctacctctcc ccactaagag aatccttatg
    15121 aaatccgttt ttacgctttc cgccagcctg gcgattagcc tgctgctgtg ctgcacggcg
    15181 caggcaaacg accataaaat cctcggcgtc attgcaatgc cacgtaacga aaccaacgat
    15241 ctggcgctga aacttcctgt ttgccgaatt gttaaacgca tacaactctc agccgaccat
    15301 ggcgatttac agttaagcgg tgcatcggtt tatttcaaag ccgcccgtag cgccagtcag
    15361 accctgaata ttccttcaga aataaaagaa gggcaaacca ctgactggat caacattaac
    15421 agcgataacg acaataaacg ctgcgtctca aaaatcacct tttctggtca tacggtgaac
    15481 tcatcggata tggccacgct gaaaattatc ggcgacgact aacgccagaa tatagaagcc
    15541 acaaaaaatg aatgttaatt acctgaatga ttcagatctg gattttctcc agcattgtag
    15601 tgaggaacaa ctggcaaatt tcgcccggct gctcacccat aatgaaaaag gcaaaactcg
    15661 cctctccagc gtactgatgc gcaacgaact gtttaaatcg atggaaggac atcccgagca
    15721 acaccgccgc aactggcagc tgattgccgg agaattacaa cattttggtg gcgatagtat
    15781 cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc attttgctcg atgtttcaaa
    15841 acgattgaag ctgaaagccg acaaagagat gtctacgttt gaaattgagc aacagttact
    15901 ggaacaattt ctgcgtaata cctggaagaa aatggacgag gaacataagc aggagtttct
    15961 gcacgcggtc gatgccaggg tgaatgagct ggaagagctg ctgccgctgc tgatgaaaga
    16021 caaattattg gcaaaaggcg tgtcgcattt gctttccagc caattgaccc gcattttacg
    16081 cacccacgca gcaatgagcg tacttgggca tggtttgctt cgcggcgcgg ggctgggagg
    16141 ccctgtaggc gcggcactaa atggggttaa agcggtcagc ggcagtgcct atcgcgtgac
    16201 aattccagcc gtactgcaaa tcgcctgcct gcgccggatg gttagtgcca ctcaggtctg
    16261 ataccggaaa ccggggaaaa ttttcatttt cccctcaaaa gatcgtagac actgccccac
    16321 tggctgatta ttatgccgcg ccctgaaaac actacagtta ttcagggaaa ttatttcacc
    16381 attcattcga tgatgatttt tgaggaatta tgggcaacac taagttggct aatccggcac
    16441 cgctgggcct gatgggcttc ggcatgacca ccattctgct taacctgcac aacgtgggtt
    16501 attttgctct ggacggtatt attcttgcca tgggcatttt ctacggcggc atcgcgcaaa
    16561 tttttgctgg tctgctggag tacaaaaaag gcaacacttt cggtttaacc gcattcacct
    16621 cttacggttc tttctggctg acgctggttg cgattctgct gatgccgaaa ctgggtctga
    16681 ccgatgcgcc aaatgcacag ttccttggtg tctacctggg tctgtggggc gtatttacgc
    16741 tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct gcaattcgtt ttctttagcc
    16801 tgaccgtgct gtttgccctg ctggcgatcg gtaacattgc cggtaacgcc gcaatcatcc
    16861 actttgccgg ctggattggt ctgatctgcg gtgccagcgc aatctatctg gcgatgggtg
    16921 aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat tggtgaatcc cattaattgt
    16981 tttgagatcc ccccgctcgg ggggattttt ttattcgcta acgtcgcgtc ttgcactctt
    17041 cggtctgaat gctgcaacca cttccggtgc cgtttcaatg tatggccctt ccagcaactg
    17101 aatgcagtac ggtacgctgg caaaaatacc atgcaccaca acgttacccg cagcgtcctt
    17161 cacgccttcc agcgtctctt taatcgactt cggctgaccg ggtaagttaa ggatcagcgc
    17221 ctgtttgcga atcacgccca cctgacgcga aaggatcgca gttggtacaa aatgcaggct
    17281 gatctggcgc atctgttcac caaagccagg catctcgcgg tccgctactg ccagcgtcgc
    17341 atcgggcgtt acgtcacgac gcgccgggcc agttccgccc gtggtgagca ccagatggca
    17401 actcatttca tccaccagct cacacaacgt ttgctcgatg atcgcctgct catcggggat
    17461 taagcgggtt tccagttcaa acggcgtggt tagcgccgat gtcagccatt cttccagcgc
    17521 agggatgcct ttatcctgat aaacgccgct ggatgcgcga tcagagatgg aaactaagcc
    17581 aatacgtaaa gtattcatca agatttttcc gtttaaacag tctcgttaaa cagaatgata
    17641 cactgcgaag ggagtgacag acaggcttca gaaaaggtag cgtgaccgac ttcccggtca
    17701 cgctaagaat gattacagca gatcgccgat cattttttcc agtttttcct ggtcaacagc
    17761 aaacttacgg ataccttccg ccagtttatc tactgccatt ggatcctggt tatgctgcca
    17821 caggaactcg gactcagtga tacgcgccgg acgcgctttc acttcgccgg tgtaagacag
    17881 tttacgttcg atagcccctt cgctctcagc cagctctttc agcagcgccg gtgcgatggt
    17941 cagacggtcg cagcctgcca gttccagaat ttcgccgatg ttacggaagc ttgcgcccat
    18001 caccacggtt tcataaccgt gctctttgta gtactggtag atttcagata cagaaaccac
    18061 gcccggatct tctgccggag cgtactcttt cttgtcggta ttcgctttgt accagtcaag
    18121 aatacggcca acaaacggcg agatcaggaa cacgcccgct tccgcacaag cacgagcctg
    18181 agcgaaggag aacagcaggg tcaggttaca gttaatacct tctttttcca gctgttctgc
    18241 agcacggata ccctgccagg tagaagccag tttgatcaga atacgatcgt tgctaatccc
    18301 tgcatcgttg tagagtttga tcaggcgttt tgctttcgca attgacgctt cggtgtcata
    18361 ggaaagacgc gcatcaactt cagttgagat acggcccgga accagtttca ggatttccag
    18421 accaatattt actgccagtt tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct
    18481 ctgctgtttc gcccaggcga cagcatcatc aatcaactta cggtattccg gaatctgcgc
    18541 tgcgttaaga atgagagaag ggttggttgt ggcatcctgc ggttgataca gcttcattgc
    18601 cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga cgaagggagg tcaatttgtc
    18661 cgtcatgata gtatttctct ttaaacagct tgttaggggg atgtaaccgg tctgccctga
    18721 tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac ggttatcgcc aggcgagact
    18781 gtttcggatt tctgacactg cgttgatatc gcccgccatt tttatacaaa acctcatgta
    18841 tgctacgcag aagttatcaa gtacctcgta gcgtatatac ttcttaaaca attggtaacg
    18901 tttacacagg aaagtcatcg cgaccggcaa taagagggat atgcatgcca gattttttct
    18961 cctttattaa cagcgtcctt tggggatcgg taatgattta cctgctcttc ggcgcaggtt
    19021 gttggttcac ttttcgcacc ggatttgtgc agtttcgcta catccgccag tttggcaaaa
    19081 gtcttaaaaa tagcattcat ccacagccag gcggtttaac ctcatttcag tcattgtgta
    19141 ccagtcttgc ggcgcgcgtg ggtagcggca acctggctgg cgttgcgctg gctattacca
    19201 ccggtggacc aggtgccgtc ttctggatgt gggttgccgc gtttatcggc atggcgacct
    19261 cgtttgccga atgttccctt gcacaacttt ataaagaacg tgacgttaat gggcagtttc
    19321 gtggcggacc ggcatggtat atggcgcgcg ggctggggat gcgctggatg ggcgttctgt
    19381 tcgccctctt tttgctcatc gcctacggca taattttcag cggagttcag gcgaacgccg
    19441 ttgcccgagc cctgagtttt tcttttgatt ttcccccgct ggtgacaggc attattcttg
    19501 ctgtctttgc tctgctggca atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg
    19561 ggtttgtccc gttgatggcg ataatctggg tactgaccag cctggtaata tgcgtaatga
    19621 atatcgggca acttccccac gtcatttggt ctatttttga gagtgctttt ggctggcagg
    19681 aagcggcagg cggcgcggcg ggatatacct taagccaggc gattactaac ggttttcagc
    19741 gcagtatgtt ttccaatgag gcgggaatgg ggtcgacgcc aaacgcggca gcggcggctg
    19801 cgtcctggcc tccgcatccc gcagcgcaag gaattgtcca gatgattggc atttttatcg
    19861 acaccctggt catctgtacg gcaagcgcca tgctgatatt actggcgggt aacggcacaa
    19921 cctacatgcc gctggaaggt attcagctta tccagaaggc gatgcgggta ctcatgggtt
    19981 cctggggtgc tgagtttgtt accctcgtgg ttattctgtt tgccttcagc tccatcgttg
    20041 ccaactacat ttatgccgaa aacaatctct tcttcttacg cctgaacaac cctaaagcga
    20101 tctggtgtct gcggatctgc accttcgcaa cggtcatcgg cggcaccttg ctaagtcttc
    20161 cgctgatgtg gcaactggca gatatcataa tggcctgcat ggctattacc aatttgaccg
    20221 ccatattact gctctcgcct gtggttcata ccattgccag tgattatcta cgccagcgta
    20281 aactcggcgt gcgcccggtg tttgatccgt tgcgttatcc ggatatcggt cgccagcttt
    20341 ctccggacgc gtgggatgat gtttcgcagg agtaatcaca actatcgatc aactcattct
    20401 ctttttttgc taaagtcggc ataaatttcc tgcaaggact ggatatgctg attcttattt
    20461 cacctgcgaa aacgctcgat taccaaagcc cgttgcccac cacgcgctat acgctgccgg
    20521 agctgttaga caattcccag cagttgatcc atgaagcgcg gaaactgacg cctccgcaga
    20581 ttagcacgct gatgcgcatc agcgacaaac tggcgggtat caacgccgct cgctttcatg
    20641 actggcagcc agatttcacg ccggagaatg cccgccaggc gattctggca ttcaaaggtg
    20701 atgtctacac cggcttgcag gccgaaacct tcagcgaaga cgatttcgat tttgcccaac
    20761 agcatttgcg aatgctttcc ggcttgtatg gcgtactgcg cccgctcgat ttaatgcagc
    20821 cttatcgtct ggaaatgggg atccgtcttg agaatgcccg agggaaagat ctgtatcaat
    20881 tctggggaga tatcatcacc aacaagctga acgaggcgct cgcagcacaa ggcgataatg
    20941 tggtgattaa tctggcgtca gatgagtatt ttaaatcggt gaagccgaag aaattgaatg
    21001 ccgagattat caagccggtg ttcctcgatg agaagaacgg caaatttaag atcatcagct
    21061 tctacgctaa gaaagcacgc ggtctgatga gtcgtttcat tattgaaaat cggctgacca
    21121 aaccagagca actgactggt tttaatagcg aaggttactt ctttgatgaa gcttcctcca
    21181 gcaacggcga actggtgttt aaacgctacg agcagcgtta agtttgcagt caatgccgga
    21241 tggcaatccg gcattgatat ttagcgatga tgtttgtcag gccccggacg atgatcgcca
    21301 cgatgatcat tgtgcttgtt atggcgcggc gatggcggcg gtccatatgg gtgccagcga
    21361 ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg accgccatcc
    21421 cagtaatagc cacgattatc acgatcgcct atctgtaatt ttactgacgg gactaacgtc
    21481 atttccgcag cctgcgcagc catgggagcg accagaacca gggaaagtgc gagtacgata
    21541 gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg
    21601 agattacgcg atgagaacgg cagttgttat tctccgcaat cctaatctct aagttaccgc
    21661 atttattgag catttctcct ttttccctgt cgtttttctc cataatttct tcataaaaaa
    21721 gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagata ttactgatga
    21781 ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgtga aagcaagggt
    21841 aaatcggcac gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc
    21901 acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga
    21961 tgcaactggt cacgcagtgc acgatacgct acggcagcgt gcggctccga agtgtagccc
    22021 agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc tgcataaccc
    22081 agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttc
    22141 ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg tgaccactga
    22201 ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc
    22261 accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa gtttccgctt
    22321 ggcaccgaga caaccagctg gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag
    22381 tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagctga gtttagcccc
    22441 agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg
    22501 aaatcgccgt cgatggcaac agtttcaata ttgccgccca atgtacagaa cagtttttct
    22561 tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt cggcaaaccg
    22621 tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag aatggtcact
    22681 ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta
    22741 aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc aacattggcg
    22801 accggagccg ggaagacaaa cgccgcgcgt acgcgctctt ccaggatttc ctgcgggatt
    22861 tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc
    22921 atctcatcaa tttcagtcag gctgaattcc ggcaggtcgt gcgggaaaaa cagcccctga
    22981 tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcatt gtgatctttc
    23041 agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa
    23101 atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc ggcaacgcgc
    23161 tgggcggtat ccggcttgtc acacagagcg aacaaagtcg ggccggagcc ggagataccg
    23221 ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc tggcagtaac
    23281 cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga
    23341 cgggaatagc aggcgtgaat aaagcctgcc agatgtcgcc cgtgcgcaat gcaatcctgg
    23401 cggcgatact gcgccggtaa aatagccctg gcttctgccg tcgagacttt aatccccgga
    23461 tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat gatgtcgttt
    23521 tcttcgatca tcaactgcat accaccgaga aaacacggtg ccacgttgtc gtaatgaatg
    23581 ctgccggaga tacgcccttc cagctcgccc atcaaagcca gcaaacgggt gtcattaagc
    23641 ggcttgccgc agtgttcatt catcgccatc agcgccgcga ccactgagca ggcgctggag
    23701 cctaagcccg agccgatcgg catattcttt tccagggtca tcgccactgg aatttgctta
    23761 cccagctcct ggcaaaaacg ctcccaacac tgataaacga tattttcccg aggttctgac
    23821 ggcagcttat cggcaaagcg tccgaggtta ttgagactga atgtctctgc cgcctcaacc
    23881 gtgactacat ctccgagcaa tgcaccatca acgggtgtca ccgccgcccc gagcacatcg
    23941 aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc
    24001 taacttccat gagagggtac gtagcagatc ggcaaagaca ccggcagctg taacgtcatt
    24061 gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc tatagaaggc
    24121 cagggcgttt tcgccgtttt tcactttgaa cagcggatcg ttgccatcca cttcagcaat
    24181 cttcacgcgg cagacgccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc
    24241 atcacgggcc ttcgccacac gagcggcaaa gagatcatcg agctgtgaca gattcgccat
    24301 aaaagcggca acatcaccct cggcgttaaa ctcagcgggt agcacaggtt caatttcaat
    24361 atccgccagc tccagttcac gtcccgtttc acgagcgaga atcaatagtt tacgcgccac
    24421 atccatacca gaaagatcat ctcgcggatc cggttcggta taacccattt cccgcgccaa
    24481 cgtggtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga
    24541 accagaaaga atgccggaga acttcatcaa ttcatcacct gcattgagca gattttgcag
    24601 gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga
    24661 tttttccgcc gcatgacgca acagatggta gtaatccatc gacgaggtgt tagccttttt
    24721 gttcggcgtg acgacgtgga aaccttcgcg caggaagtcg gcatattgat ccgccactgc
    24781 ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg
    24841 aattaagcgc ccgagattaa acggctcttt ggcttgcgcc agttcttcct gccagttttc
    24901 cagattaagg ccatgtacat tggtgagcag agccttcgag ttggcaacgc cgcagacacg
    24961 taagtcgata tgtttattct tcaaccagct ttgctgacgc ttcagttgct ccagcagcgc
    25021 accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag
    25081 catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat
    25141 tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagcgc
    25201 ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat
    25261 aatggccagc cgttccgtca ccgccagcgg ctccagtaag ccttctttca gttccaggta
    25321 gaactcttcc tgcattgccc gttcagctcg cacacagtcg ctttgtggaa cgcagaaact
    25381 gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat
    25441 cgctgcaaag acgcgcgccg ccatgccgac catccctttc atccccggac cagaaacgct
    25501 gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgt cttcatcacg
    25561 gctggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg
    25621 gatctggaac tgggcgatgg gggtaattgt gcgggggtga agaactttag cgccgaagta
    25681 ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg
    25741 acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg
    25801 taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag
    25861 ttcgcctttt tcattaccgg cggtgaaacc tgccatcaac accatgtgat cagccggaat
    25921 acgacttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt cgaggtaatg
    25981 ccccactgcc agcagttttt cgaccggatc gataacggta acgttgtgac cacgcgcttc
    26041 taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt
    26101 gatgctatcc gggcactgcc ccaacaaact aatgccatgc aggacatgtt ttatttgggc
    26161 aaattcctgg tcgacgaaag ttttcagttg cgccagcggg aatcccggct gagcggcggc
    26221 gagtcccgtc agaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc
    26281 ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt tggcgggggc
    26341 agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacccg
    26401 cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt
    26461 tacctcgtta cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt
    26521 gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg
    26581 gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tctgtgcgct
    26641 atgcctatat tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg
    26701 gcaggtaacc agttcagaag ctgctatcag acactctttt tttaatccac accgagacat
    26761 attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt tttttatttg
    26821 gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct
    26881 aaaagcccca ggcgttgttg taaccagtcg accagtttta tgtcatccgc cactgccaga
    26941 gtcgtcagca gtgccatggc tcgttcgcgt aaagcttgca gttgatgttt gtctgccgtt
    27001 gtgtcacttt tcgccggttg ttgtattaat gttgctaatt gatagcaata gaccatcacc
    27061 gcctgcccca gattgagcga aggataatcc gccaccatcg gcacaccagt aagaacgtca
    27121 gccaacgcta actcttcgtt agtcaacccg gaatcttcgc gaccaaacac cagcgcggca
    27181 tggctcatcc atgaagattt ttcctctaac agcagcacca gttctactgg cgtagcgtag
    27241 taatgatatt tcgcccgact gcgcgcagtg gtggcaacag tgaaatcgac atcgtgtaac
    27301 gattcagcca gtgtcgggaa aactttaata ttatcaataa tatcaccaga tccatgtgcg
    27361 acccagcggg tggctggctc caggtgtgcc tgactatcga caatccgcag ctcgctaaac
    27421 cccatcgttt tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc
    27481 agaataatcg ttatacgcat attgccactc ttcttgctca aataaccgcg aaccgggtga
    27541 ccactgtcaa cttattacgc ggtgcgaatt tacaaattct taacgtaagt cgcagaaaaa
    27601 gccctttact tagcttaaaa aaagctaaac tatttcctga ctgtactaac ggttgaattg
    27661 ttaaaaaatg ctacatatcc ttatgtttac ttaggataat tttataaaaa ataaatctcg
    27721 acaattggat tcaccacgtt tattagttgt atgatgcaac tagttggatt attaaaataa
    27781 tgtgacgaaa gctagcattt agatacgatg atttcatcaa actgttaacg tgctacaatt
    27841 gaacttgata tatgtcaacg aagcgtagtt ttattgggtg tccggcccct cttagcctgt
    27901 tatgttgctg ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg
    27961 aaagtaccca cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg
    28021 caattacgta ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag
    28081 gtcagggact tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca
    28141 gaccccgcac attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat
    28201 tttcgaagcg gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat
    28261 cctctctgaa tatgacatca acctggtgat catggatatc aatctgccgg gtaagaacgg
    28321 tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg
    28381 ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac
    28441 caaaccgttc aacccgcgtg aactgactat tcgtgcacgc aacctgctgt cccgtaccat
    28501 gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg
    28561 ggaactggac atcaacagcc gttcgttgat cggccctgat ggcgagcagt acaagctgcc
    28621 gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtcccg
    28681 tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga
    28741 cgtgacgatc cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat
    28801 cgccaccatt cacggtgaag gttatcgctt ctgcggtgat ctggaagatt aatcggcttt
    28861 accaccgtca aaaaaacggc gctttttagc gccgttttta tttttcaacc ttatttccag
    28921 atacgtaatt catcgtccgt tgtaacttct ttactggctt tcattttcgg cagtgaaaac
    28981 gcataccagt cgatattacg ggtcacaaac atcatgccgg ccagcgccac caccagcaca
    29041 ctggttccca acaacagcgc gctatcggca gagttgagca gtccccacat cacaccatcc
    29101 agcaacaaca gcgcgagggt aaacaacatg ctgttgcacc aacctttcaa taccgcctgc
    29161 aaataaatac cgttcattat cgccccaatc agactggcga ttatccatgc cacggtaaaa
    29221 ccggtatgtt cagaaagcgc cagcaagagc aaataaaaca tcaccaatga aagccccacc
    29281 agcaaatatt gcattgggtg taaacgttgc gcggtgagcg tttcaaaaac aaagaacgct
    29341 ataaaagtca gtgcaatcag cagaatggcg tacttagtcg cccggtcagt taattggtat
    29401 tgatcggctg gcgtcgttac tgcgacgcta aacgccggga agttttccca gccggtatca
    29461 ttgcctgaag caaaacgctc accgagatta ttagcaaacc agctgctttg ccagtgcgcc
    29521 tgaaaacctg actcgctaac ttcccgtttg gctggtagaa aatcacctaa aaaactggga
    29581 tgcggccagt tgctggttaa ggtcatttcg ctattacgcc cgccaggcac cacagaaaga
    29641 tcgccggtac cgcttaaatt cagggccata ttcagcttca ggttctgctt ccgccagtcc
    29701 ccttcaggta aagggatatg cacgccctgc ccgccttgct ctaagccggt gccgggttca
    29761 atggtcagcg ccgttccgtt aacttcaggt gctttcacca caccaatacc acgcgcatcc
    29821 ccgacgctaa tcacaataaa tggcttgcct aaggtgatat ttggcgcgtt gagttcgcta
    29881 agacgcgaaa catcgaaatc ggcttttagc gttaaatcac tgtgccagac ctgaccggta
    29941 taaatcccta tcttgcgttc ttccacgttc tgattgccat caaccatcaa tgactcaggt
    30001 aaccaaaaat ggataaaact tcgtttccgc tccacggttt tatcctcttc ctgcaccgta
    30061 taaagctcgg tcacaggaat agcgatgagc ggcccaacga gtttttgcgg cccgctggta
    30121 ctttggcgaa tcgcatcttc cacatcgcta cggtaatcag cacgttcgac aatcacctgc
    30181 cgaatcagca ttatcggaat caacaacagc aatactgcac caaacagggt ggtcattttc
    30241 cagaacaggg gggatttcaa catagtcttc tcctttgcaa tggcagcagg atacgaaggc
    30301 tatgtgggaa gaatttgaag ctatgtgaag tgacggtgaa gtcgaagcga agccagcacg
    30361 ccaccttcct gcacattgcg cagcgtgact tcgccgttaa acaaacgggc gacctcactg
    30421 acgaacgcca accccagacc gctgcttttt tgcccatttg cacgaggcaa agagtaaaag
    30481 cgttcaaaaa tacgtgaaag cgcgtagtca ggaataccac taccggtatc cagcacctta
    30541 agcgtgacgt gttcctgctc cacttcggcg cttagcgtga tgcaaccgct ctcgggggta
    30601 aaatcgatgg cgttatccag taaattcccc agagcctgct ccagtaacgc cggttcagca
    30661 gcaacgttaa cctcggtggg cgtaacatgt agagtgatgt ttttttctgc caactgcacg
    30721 gtgcgcgctt cgctgacgcg gcggaataat gccgccacat caacagcagt tagaacgact
    30781 tcctgacgat tctccagtct tgcctggcgt agtaacgttt ctaccaatgc ctgcatacgc
    30841 gcattttgcg tcagaatgtt gtcagtaaaa cgagccacca cttccggcgg cggaccttcg
    30901 cgtaaaattt ccgccgctcc acgaatcgcc gccagtgggc tttttagctc atgagttaat
    30961 gcgtaaacat actgctcaat atagtttttc ccttccagct tcacgcgcat actttccagc
    31021 gcctgcgcga gtttacgcag ctcgctacta ccgagatcgg ggagaggaac gggcttattg
    31081 tcagtgacgg aatcagcata gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc
    31141 atgcctgcgc caatcaccag tgcaatcccc aacaaaatgg cgctggccca taaaattcgc
    31201 cgctcgctgc gtttaatgac cggagccatc gccgcgttcg gtttgcctac gctcaaaacg
    31261 ccaataagcc gcgagccgtc cataatcggc gcggcaacat acatcaccga actttcggga
    31321 tcggtaggat tttgcaacgt gctgcgcgca ccatactgac cacgcaacgt tagccagacg
    31381 tcattccagc gcgaataatc ctgtccaacg gctttatttg ccgaatcgaa caatactttg
    31441 ccctgcgcat cggtcatata gacatgatat tcattgcgca ctttgttaat gccaccgata
    31501 ttggcgcgaa acgggcgatg ctgtagctga ttaaacgcct gcgccagttg cccatgcgtt
    31561 gtgtccccag agagcaaatc gggacgcgcc agctccgcca gcaacgttgc ggtgtcgatc
    31621 aacgtcccct ccgttgctct tcgcacgccc ggtttaactt ctttgacaaa aatggccagt
    31681 acgaaccaag ctgccaccgc caccagtaaa aaatagccca gcaacaaccg catgccgata
    31741 cgcattacag gcccctcagg ctatatccca tgccgcgatg agtattaatc ggtgaaagat
    31801 cggggttgat ggcgcgcagc ttggcacgca gcgttttaat gtgggtatcg acggtgcgat
    31861 cgtaggtgtc ctgcgcatct tcccacacgc tatccatcag ttgctggcgg gaccagacgc
    31921 ggcctggcga cttgagtaac gtcttcagca ataaaaactc ataccgcgtc agcgttaatg
    31981 gcgtgtcaaa ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga
    32041 tgacgggaga cggcgtcgag aacttcttca cccgacgcag taaggtgcgc accctggcgc
    32101 acacttcgcg gggtgaaaac ggtttagcca catagtcgtc agcgccaatt tccagcccaa
    32161 gcaggcgatc gacctcttca cttcgggcgg tcaggaacag tacaggtaac gccggatgga
    32221 gcgtcagtaa ctggcggcac aattcaaagc cactgatatc aggcaaacca acgtcgagaa
    32281 tcatgacatc gggaacctgc tggcgagctt tatccagcac cggcaagcct cgctcaaaga
    32341 cctcgacggc aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct atcccttgct
    32401 catcttccac taaccagacc gtttcccgtt gcataaatcg cctctgttat tgccgccacg
    32461 gcatgacagg aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt
    32521 aagccagata agcgagcgcg ttgcgtttag cgtcataaaa gcgcacgacc tgtaacgact
    32581 taaagaccag ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg ccgtttttaa
    32641 tacgatccga cagttcaatc ggcccgactt gctgacaaga aatggccgca tcggaggtat
    32701 cttctgccag gcccaatccc cctttaatac caccggtttt cgcccgactc acataacagg
    32761 tgacattttt cacatcggga tcgtcaaagg cttccacaac aattttgtga tccgggccga
    32821 tcattttaaa tacggtgtcg accgaaccaa tctcttctgc atgagccaat ggccccagca
    32881 ttatcaggct taaagacagg atcaaatgct tgtatttcat attgttacca tttttatcta
    32941 ttacacgagc aattagttcg tcacggtaaa taacatattg attaagaaca aaatctcaat
    33001 acttttattt ccgtcaagcc ctaaaacata ctctactaag aaaaaaacac tgaatgctaa
    33061 aacagcaaaa aatgctatta tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg
    33121 aggatatttt atggatcagg ccggcattat tcgcgacctt ttaatctggc tggaaggtca
    33181 tctggatcag cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca
    33241 cttacagaga atgtttaaag atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg
    33301 tcgtttgtcg aaatcggcgg tcgcactacg cctgactgcg cgtccgattc tggacatcgc
    33361 gctgcaatac cgcttcgact ctcaacagac atttacccgc gcattcaaga agcagtttgc
    33421 ccagactcct gcactttacc gccgttctcc tgaatggagc gcctttggta ttcgcccgcc
    33481 gctacgtctg ggtgaattca ctatgccaga gcacaaattt gtcaccctgg aagatacgcc
    33541 gctgattggt gttacccaga gctactcctg ttcgctggag caaatctctg atttccgcca
    33601 tgaaatgcgt tatcagttct ggcacgattt tctcggcaac gcgccgacca ttccgccggt
    33661 gctctacggc ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac aagaagtatt
    33721 ctataccacc gcgttagccc aggatcaggc agatggctat gtactgacgg ggcatccggt
    33781 gatgctgcag ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca
    33841 ggagtttatc ctgacggtat acgggacgtg catgccaatg ctcaacctga cgcgccgtaa
    33901 aggtcaggat attgagcgat actacccggc agaagatgcc aaagcgggag atcgcccaat
    33961 taatctgcgc tgtgaactac tgattccgat ccgtcgttaa cgctgcagct catctaatgc
    34021 aggggcgtcc agatgcgaga tgtcccctgc ggtttcaacg acccagccag acgccagcca
    34081 caggctttcc tgataatcca cgcgcgaaat agaacagtta cgcagacgta agcggcgctc
    34141 tgcccatgct ggtaatccga gaatcgtact caccagacat cccagtgcaa taccatggct
    34201 caccagcagc ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg ccgcattgac
    34261 gcgatcgctg agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt
    34321 gaccagctgc cgacgccagt tctcttcttc ttcggtgagt gaatcgatat ttcttgtttc
    34381 cagtacaccc atatttaatt cacgcaggcg agaatcaaag atgatatcac agccgcaggc
    34441 ctgggcgatg atttccgccg tgcgccgggt ccgtcctaaa tcgctactga tgatatgcgt
    34501 aatgccaagc tctttggcac gggttgccac ctgcatcgcc tgttgctcac ctttggcggt
    34561 cagcgggctg tcagactggc cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg
    34621 gcggactagg tatacctgta acatgctttt tttccgtata ctgctgtaac gttaattttc
    34681 tgagtaatgc tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag
    34741 gccattctgc aggcatttca cgagatcttc ggcgaaggat cctgccatat tgcatccgtt
    34801 gccgtcgaga gcggtgtacc ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca
    34861 cgaaatcggg tagcaaatgc ccgccgttta catccagagg ctgatttttg ggtggcgatt
    34921 gaagcaggca ttgatggcga cagcaccttc agttgggtgg tgattgaaaa cgccagccag
    34981 cgcggcgagg cgcgttcggc aactttgccg ttacctgcgg tgattctgga aaaagtgcgt
    35041 gaaggcgagg cgctcggtcc cgtgatgtca cgttataccg gtattgatga gattggtcgt
    35101 aaggaagggg cgatcggcgt gtttaccgcc gggaaactta cccgcgccag cgtctatcac
    35161 caggcggtga tcctggcgct gagtccgttt cataatgcgg tgtatcagtg atctttcagc
    35221 aacacctctt ccagccactg gcgcagctcc acgggcgcgg ctttcaggct gttagatcca
    35281 cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg gctcatttcg
    35341 ccgcgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc
    35401 gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg
    35461 tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat
    35521 tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt
    35581 actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt
    35641 ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag
    35701 ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca
    35761 cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt
    35821 tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt
    35881 aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg ctgctataac
    35941 caggaataga gaacatcttc accgtatggg tcgccgtgcc cggcataatc tgcatcaggc
    36001 cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg
    36061 ccatcgcata gctttgcggg atctccttcc cactggtgta acgtttgaaa agatcgttgt
    36121 aggccagcgg gaatctctct tccagatgat cccaaagctt cccggcgatc gttgcctgaa
    36181 cgctaagttc ccaccattgg ttattgaaag cataccgagc cagttgcgcc tgctctgttt
    36241 ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg agattccagt
    36301 acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct
    36361 gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct gcaaccatcg
    36421 ggtagaaacc acgctgttgc atgagttgat gcaaaatctc ttttgcttca gcttcacgtc
    36481 cgcgttccag cagtaaatcc gcctgccagt aacgccattc atctttctct ttcgcctcca
    36541 tcggcaaacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca agcgccattc
    36601 gcacgcgccg ttcaataagc gaagtagatt gcgaacgcat aatggcgtca tcgcgccatt
    36661 tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata tcgcgcagct
    36721 cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg
    36781 cattttcagc atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa
    36841 aatcggtcgc tccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg
    36901 aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta
    36961 cgagtccagt gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat
    37021 cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct
    37081 tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc
    37141 atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg
    37201 ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg
    37261 tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt
    37321 gattcatcag gtcatcggtg atctgacggt attccaggta gggataaagc ggataatctt
    37381 tcagtccagg catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga
    37441 tttgggcgta gcggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg
    37501 tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa
    37561 gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct
    37621 aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttat actctaaata
    37681 attcgagttg caggaaggcg ataagtgagt gagtccccag gagcttacag aagtaagtga
    37741 ctggggtgag cgagcgtaag cgcagcacat gcaacttgaa gtatgacgag tatactgcga
    37801 gggtgatcga ccggctggga ttagataagg aaacaggcta cacttcgcct ttgaaaactc
    37861 atcatcacga taaacataga ggcgaagtcc aacgtggctc aattcgttta taccatgcat
    37921 cgtgtcggca aagttgttcc gccgaaacgt catattttga aaaacatctc tctgagtttc
    37981 ttccctgggg caaaaattgg tgtcctgggt ctgaacggcg cgggtaagtc caccctgctg
    38041 cgcattatgg cgggcattga taaagacatc gaaggcgaag cgcgtccgca accggatatc
    38101 aagattggtt atctgccgca ggaaccgcag ttgaacccgg aacacaccgt gcgtgagtcc
    38161 attgaagaag cggtttctga agtggttaac gccctgaaac gcctggatga agtgtatgca
    38221 ctgtacgccg atccggatgc cgattttgac aaactggccg ctgaacaggg ccgtctggaa
    38281 gagatcattc aggctcacga cggtcacaac ctgaacgttc agctggagcg tgcggcggat
    38341 gcgctgcgtc tgccggattg ggacgcgaaa atcgctaacc tctccggtgg tgagcgtcgt
    38401 cgcgtggcgt tgtgccgcct gctgctggaa aaaccagaca tgctgctgct cgacgaaccg
    38461 accaaccacc tggatgccga atccgtggcc tggctggaac gcttcctgca cgacttcgaa
    38521 ggcaccgttg tggcgattac ccacgaccgt tacttcctcg ataacgttgc gggctggatc
    38581 ctcgaactgg accgcggtga aggtattccg tgggaaggta actactcctc ctggctggag
    38641 cagaaagatc agcgtctggc gcaggaagct tcacaagaag ctgcgcgtcg taagtcgatt
    38701 gagaaagagc tggagtgggt acgtcaaggt actaaaggcc gtcagtcgaa aggtaaagca
    38761 cgtctggcgc gctttgaaga actgaacagc accgaatatc agaaacgtaa cgaaaccaac
    38821 gaactgttta ttccacctgg accgcgtctg ggcgacaaag tgctggaagt cagcaatctg
    38881 cgtaaatcct atggcgatcg tctgctgatt gactccctga gtttctcgat cccgaaaggg
    38941 gcgatcgtcg ggatcatcgg tccgaacggt gcgggtaaat cgacgctgtt ccgtatgatc
    39001 tctggtcagg aacagccgga cagcggcacc atcactttgg gtgaaacggt gaaactggca
    39061 tcggttgatc agttccgtga ctcaatggat aacagcaaaa ccgtttggga agaagtttcc
    39121 ggcgggctgg atatcatgaa aatcggcaac accgagatgc caagccgcgc ctacgttggc
    39181 cgctttaact ttaaaggggt tgatcagggt aaacgcgttg gcgaactctc cggcggtgag
    39241 cgcggtcgtc tgcatctggc gaagttgctg caagttggcg gcaacatgct gctgctcgac
    39301 gaaccaacca acgacctgga tatcgaaacc ctgcgcgcgc tggaaaacgc cctgctggag
    39361 ttcccgggct gcgcgatggt tatctcgcac gaccgttggt tcctcgaccg tatcgccacg
    39421 cacatcctgg attaccagga tgagggtaaa gttgagttct tcgaaggtaa ctttaccgag
    39481 tacgaagagt acaagaaacg cacgctgggc gcagacgcgc tggagccgaa gcgtatcaag
    39541 tacaagcgta ttgcgaagta atgcgtaaaa tgccggatgc ggcgcgccag catcgcatcc
    39601 ggtattagtt aaatgattaa tgggaggcaa gttcccggaa aaagtcagga ttttctttaa
    39661 ttgtggcaag tacttttgca gccaatcccg taggatttct tcgcttcgat tcccagcttt
    39721 taatggtatc cacactggtt cccagagctt ttgccatctc actttgcgaa acgttgagtt
    39781 gctcacgaat agctttaaca tccgcaattt cgtagcgggt cacccgtgca ggttctttta
    39841 aaccttgttt aatctcgaca gcctcttcca gagaggtttt caactcatca aaaaaactca
    39901 ttttatatct cgcctttgag cagtttcgtc aacttcttta gttgagcttt ttccgtatct
    39961 gtcagactat ctttggcatt tttcggatag gccataatca aataaataat ctcttccgtc
    40021 gccagaaagt aaattattct gacactgcca ctttttccct gtgaacccgc agccattcgg
    40081 actttacgca atccaccagt ctgctgaatg agatcacctt tatcgggagt tccgatgagt
    40141 tctttttgta attcctttaa ttcatcatcg gttgcaatct gcttgatctg acgcgtaaac
    40201 attggcgtct ctataaatat tatcgcctta cccattaccc ctccctggaa ggtacattgt
    40261 acaccaaaat ggagtactaa gtacactttt gaatcagtaa tcccactcaa catcttataa
    40321 agatgattat ctttgctccc ccatcatctc ccgcaccagc tccacgcagc gcaggaaacg
    40381 gctgtcataa tcgtcttctt caacccgcac aaattcgata ttgttctctt ccagcatctc
    40441 caccagcaag ttctggaact ctttgcgatc caccgaactg ccgaggctgc gtaaaccatc
    40501 cgccacccaa ggggtgttgt tctccagcaa gataaccaga tcaaaacggt attcatcgat
    40561 cagcgcctgc acgaacggat gctcccgccc ttcgtacttt ttgcagaacg cctgggtagt
    40621 gacaaaatca gtgtcgataa acgccacttt attggcatat ttcactgcaa aatcaatgta
    40681 ttgtgcgtgt cccagcgcga ttttatcgta gtcggaatac tgcaatgcga tctcatcacc
    40741 gccgaggtgt gaaaagacat aatcgcgacc atattcccac gcactggtgg tattgaagat
    40801 attggcaagt ttgtttacca gggtggattt accgctcgac tcgccgccaa ggatcgccac
    40861 ggtacgcaca aaaaacggct tcacttcagt ggggatatat tcccagtagc ggaacgggtt
    40921 ttcgcggatc tgcgcaccgc tgatactcat aaaggtgcgt ttcggatcga ccagcaccgt
    40981 ttcgatcccc agatgttcca tatactgtgg cgcatcggct tcttccgagg tgtagatcag
    41041 gtccggctgg atcccctttt cagccataaa ctttttgatg ccgttgctcc atacatccca
    41101 gccgtgcgga tatggctcca tgccctcttc attgaaagca tgaatacgga tatttttctg
    41161 atatttaaag gtttgcaata accagcgcag acgatccggc accgtgggct gctgcgacat
    41221 ggcgctgtct tcgaacaacg cgcggtcgcg ggtatcgtca aagcccataa tgatatgcag
    41281 ctcgtcaacc tggctacagg cgcgctggat aaggtagata tgtccggtat gcagcgggta
    41341 gaacttaccg aatacgacac caatggtttt cttctgtcgg ggaaattcaa gccccaaaaa
    41401 acggtgcaac gcctccagct tttgcgcgct ggggctttta attttggcat tcagtaactg
    41461 gcttaaatac cctttggtca taccgctggc atcagcgacc tgctgtagcg tgcagccctg
    41521 ttgcttgatg gcagttttca ggtaatcaaa tgacgacata tctccctccg tatctctcat
    41581 tataagtcgt cgaacacgct aagcgcgtcg gagagttttt taacgccaaa aatctgcatc
    41641 ccttccggcg cttttttcgg cacgttagcc gccggaacaa tcgcccggcg aaaaccgtgt
    41701 ttcgccgctt cagaaattcg ttcctggccg ctgggcaccg ggcggatctc ccctgccagc
    41761 ccgacttcac caaacaccac cagatcctgc ggcagcggtc tgtcgcgcag gctggaaacc
    41821 atcgccagca gtaacgctaa gtcggcgctg gtttccgtta ccttcacgcc gccgaccacg
    41881 ttaacaaata catcctgatc ggccatttgc aacccaccgt gacggtgcag caccgccagc
    41941 aggattgcca gacggttttg ctccagccct actgcgacgc ggcgtgggtt cgccatcatc
    42001 gagtgatcga ccagcgcctg gatctccacc aacagtggac gcgttccttc ccacactacc
    42061 atcactgagc taccggaggt cacttcatca ccgcggctta aaaagatggc ggaagggttg
    42121 ctaacttcac gcagcccctg ttcggtcatc gcgaagacgc ccagctcatt caccgcgccg
    42181 aagcggtttt tatggctgcg taaggtgcga aaacgagagt cggcatcgcc atccagaagc
    42241 accgaacagt cgatacagtg ttccagcact ttagggccag ccagcgagcc atctttggtt
    42301 acgtgcccaa ccatgacaat cgccacgccg cgcgttttgg cgaagcgcgt cagataagcc
    42361 gccgtttcac gcacctgcgc cacgctgcca ggcgatgact gaacatccgc catatgcatc
    42421 acctggatgg agtcaattac catcagcttc ggttgctctt cttcggcaat caggcagatc
    42481 tgctcgatgc tggtttccga cagcatatta agattgtcag tcggcaggcc aaggcgatga
    42541 gcgcgcattg ccacctgttg cagcgactct tcgccagtga catacagcgt tttcatctgc
    42601 tgcgccagtt tgcacagcgt ttgcagcaac agcgtggatt tccccgcacc tgggttaccg
    42661 ccaatcagaa tggcgcttcc cggcaccacc ccgccgccta atacgcggtc gaactcttta
    42721 aatccggtgg aaaaacgcgg cagctcctca aggctgatgt cggaaagttt ctggactttt
    42781 gccactccgg cgctaccggc atagccactg agacgctcgt tacgcgccac cgttggcgac
    42841 gcagcaagac gcacctcggt gatggtgttc caggcatgac aggcgctgca ctgcccctgc
    42901 cagcgcggat aatcggcccc gcattcatta caaacaaagg cgcgttttgg agcttttgcc
    42961 acggtgtacc tcgttaatgc tgtgccgccc gcaggatgac gggcaggcaa ttacttctga
    43021 ttcaggctgc cagagaggat gcagaatacc cccatcaggt cagcgtgacg gatggtgact
    43081 tccgtctttt cattcacttt tggcttggca tggtaggcaa tccccagccc tgccgctttg
    43141 atcatcggca ggtcattggc tccatcgcca atcgccacgg tctgcgccag cgggatttca
    43201 tactcctgcg ccaggcgggt cagggttttc gctttatatt gcgcgtcaac gatgtcgccg
    43261 atcacattgc cggtaaattt accgtccatg atctccagtt cattggctac cactgctgtc
    43321 aggcgcagct tgtcgcgcag gtattcagca aagaaagtaa agccgccgga ggcaatcgcc
    43381 actttccagc ccagcgtttc cagcttgagt accagctgcg ttaagcctgg catcagcggc
    43441 agattttcac gcacctgttg cagaatattg gcgtcagcgc ctttcagcgt cgccacgcgg
    43501 ctgcgcaggc tggcggtaaa atcgagttcg ccgcgcatcg cccgttcggt tacctccgcc
    43561 accctctcgc ccgttccggc cagtttggca atttcatcaa tacattcaat ctggatggcg
    43621 gtggaatcca tgtccatcac cagcaaaccc ggcgtgcgca ggtgcgggat tttccccagc
    43681 ggcgcgacat ccagctgcgc ttcgtgggcc aggcgcgtag cccgcgcggt gagtgaacct
    43741 gccagacgaa tcacctgata atcttccacg caccaggcgg caacaatcac catcgccgcg
    43801 cccagtttgc tctggtattg ggtcagacgt tgtttatcca gcccacgacc atacagcagc
    43861 cagccgctac gacctgcgtg gtaatccagt ggcatcactt catcaccact taatgaaaga
    43921 ggcagacccg gccataaaga gacatcttca ggcaggtcgc accaggtaat gttaggcatt
    43981 aaggctcctg taaaatcgtt cgaagcaggg aaaataacgc atgaggctac cttgtatcca
    44041 ttgcttctgg caacattaag tctcaaattt tcaaagggtg gaagatggct cgcacaaaac
    44101 tgaaattccg gctgcatcgg gcagtgattg tcctgttctg tcttgccttg ttagtggcgc
    44161 tgatgcaggg agcgtcatgg tttagtcaaa accaccagcg acagcgtaat ccacagctgg
    44221 aagaactggc ccgcaccctg gcgcgtcagg tgacgctgaa cgttgcaccg ctgatgcgta
    44281 ccgactcacc ggatgaaaaa cgcattcagg cgatccttga tcagttaacg gatgaaagcc
    44341 gtatcctcga cgcgggcgtg tatgacgaac aaggcgatct tatcgcccgt tctggcgaaa
    44401 gcgtcgacgt gcgcgaccgg ttggcgctcg acggcaaaaa agcgggcggc tattttaacc
    44461 agcagattgt cgaaccaatt gccgggaaaa acggaccgct cggctatctg cgccttacgc
    44521 tcgacaccca tacgctcgcc accgaagccc aacaggtgga taacaccact aacattttac
    44581 gcctgatgtt gctgctctca ctggcaatcg gcgtagtgct gacccgcacg ctgctacagg
    44641 gtaaacgcac ccgctggcag caatcgccct tcctgttaac cgccagtaaa ccggtgccgg
    44701 aagaggaaga aagcgagaaa aaagagtgac ccattactac aagaaaggaa atcgttatgt
    44761 ccacattacg cctgctcatc tctgactctt acgacccgtg gtttaacctg gcggtagagg
    44821 agtgtatttt tcgccagatg ccggctacgc agcgcgttct gtttctctgg cgcaatgccg
    44881 acacggtagt gattggtcgc gcgcagaacc cgtggaaaga gtgtaatacc cggcggatgg
    44941 aagaagataa cgtccgcctg gcgcggcgca gtagcggtgg cggcgcagtg ttccacgatc
    45001 tcggcaatac ctgttttacc tttatggctg gcaagccgga gtacgataaa accatctcca
    45061 cgtcgattgt gctcaatgcg ctgaacgcgc tcggcgtcag cgccgaagct tccgggcgta
    45121 acgatctggt ggtgaaaacc gccgaaggcg atcgcaaagt ctcaggctcg gcctatcgcg
    45181 aaaccaaaga tcgtggcttc caccacggca ctttgctgct caattccgac cttagccgcc
    45241 tggcaaacta tctcaatccg gataaaaaga aactggcggc gaaaggcatt acctcagtgc
    45301 gttcccgcgt gaccaatctg accgaattgc taccggggat cacccatgag caggtttgcg
    45361 aggccataac cgaggccttt ttcgcccatt atggcgagcg cgtggaagcg gaaatcatct
    45421 ccccggacaa aacgccagac ttgccaaact tcgccgaaac ctttgcccgc cagagtagct
    45481 gggaatggaa cttcggtcag gctccggcat tctcgcatct gctggatgaa cgctttacct
    45541 ggggcggcgt ggaactgcat ttcgacgttg aaaaaggcca tatcacccgc gcccaggtgt
    45601 ttaccgacag tctcaacccc gcgccgctgg aagccctcgc cgggcgactg caaggctgtc
    45661 tgtaccgcgc ggatatgctg caacaggagt gcgaagcgct gctggttgac ttcccggaac
    45721 aggaaaaaga gctacgggag ttgtcagcgt ggatggcagg ggcggtaagg taactcgctg
    45781 attaaaatat ccagggaagg aaatatgatc gatctggaaa accaggaacg ggagattatc
    45841 aatttaatgt tcagccaggg tatatcctgg ctaaccgccg tgcgtatcag gcataaacta
    45901 tcacttgctg aagtaagtaa aatgttaggt atctccatta attctctaaa acagatcgaa
    45961 aagacggaac ggcttagttc aaacatcaaa agcaaaatgg caggaattta tggttgtcca
    46021 ccagagttgc tgatatgccc ttcctggatg acggctgaac ataaatgaat agattcgtga
    46081 atagatactt gaatggattt tattttctat ccatatgaaa aataatagta aatctataaa
    46141 tcagttgcta ttgatgaata aatactaaaa agccggagca gtctcccact ccggcttcac
    46201 acgacaatcg cctttcagca aaacacaatt actctttatc gcccagcaga acggattcca
    46261 gcgcgatttt gatcatgtcg ttgaaggtgg tctgacgctc agcggcagtg gtctgctcgt
    46321 gagtgcggat gtggtcagac acggtgcaga tggtcagggc tttcgcgcca aattctgcag
    46381 cgacgccgta gataccagcc gcttccattt ccacgccgag gatgccgtat ttttccatca
    46441 cgtcgaacat ttcgccgtcc ggagagtaga acaggtcagc ggagaacagg ttaccaacgc
    46501 gagcatcaac gcccagtgct tttgccgcgt ctaccgcgtt acgcaccatg tcgaagtcag
    46561 cgatagcggc aaagtcatgg tctttaaaac ggatgcggtt aactttggaa tcggtgcagg
    46621 cacccatacc gataacgacg tcacgcagtt ttacgtgcgg cagaacagca ccacaggaac
    46681 ctacgcggat aattttcttc acgccgaaat cggtgatcag ttctttggtg tagatggagc
    46741 aggacgggat acccataccg tgacccatta cggaaatttt gcggccttta taagtaccgg
    46801 tgaagcccag cataccgcga acgttgttca cttcacgggc atcttcaagg aaagtttcag
    46861 caatatactt cgcacgcagc gggtcgcctg gcatcaaaac tacgtcagca aaatcgccca
    46921 tttctgcatt aatgtgtgga gtagccatcg ttttatcctt tttgtgacat attaaaggcg
    46981 gagtcactgc tccgcccaaa tccatcagaa catggctttg ccatattcca tatcagaagt
    47041 accaaaatat tttgccagag tctggccgat atccgcgaag gtttcacgat gccccagcga
    47101 acctggtttc actttcgggc cgtaaaccag taccggaatg tgttcacgcg tgtggtcagt
    47161 accggtccag gtcgggtcac aaccgtggtc agcagtgaga atcagaatat cgtcatcgcg
    47221 cagcaaagac atcagctccg gcagacggcg gtcgaacagt tccagacccg cagcataacc
    47281 ggcgacgtcg cgacggtggc cccaggaaga gtcgaagtca acgaagttgg tgaagacgat
    47341 ggtgttatca cccgcttctt tcatctcttt gatggtggcg tcaaacagcg cgtccaggcc
    47401 ggtcgctttc acttttttgg tgataccgca gttggcatag atgtctgcaa ttttaccgac
    47461 cgaaaccacc tggccgtgtt tttcatcaac cagtttctgc agcacggtcg gtgctggcgg
    47521 ctcaacagcc aggtcgtgac ggttaccggt acgctggaag ttaccggctt tgtcgccgat
    47581 aaacggacga gcgataacac gaccaatgtt gtagccgccg ttggtcagct cttcacgggc
    47641 gatttcgcac agttcgtaga gtttatccag accaaaggtt tcttcgtggc aagcaatctg
    47701 gaacacggag tcagcggagg tatagaaaat cggcttgccg gttttcatgt gctcttcgcc
    47761 cagttgatcc agaatgaccg taccggaaga gtggcagtta ccgaggtaac ccggcagatt
    47821 agcgcgttca accagtttat ccagcagctc ttgcgggaag ctgttttcgt gatcggagaa
    47881 atatccccac tcaaacagaa ccgggacacc ggcgatttcc cagtgaccag acggggtgtc
    47941 tttaccggat gacatttcgt gcgcccatgc gtacgcgccg ataacttcag cgttgccatc
    48001 cattcccgcc ggaatgaaac cggtagaacc ttcgtgtgct ttcgccagcc caagacgggt
    48061 cagatttggc agattgagcg ggcctttacg accgtggtca gcttcgcctt tggcacaagc
    48121 ttctgcgata tgacccaggg tgtcagcccc gacgtcacca aagcgttctg catcttctgt
    48181 agcgccgatg ccgaatgagt ccagcaccat aataaatgca cgtttcatat gttctccgta
    48241 ctcagtgctt caaatttaaa tgatcagatc agtataccgt tattcgctga tacggcgata
    48301 gacagttggt gtgctttccg gtgctttatc ggcaagttta attgccgctt tcaccgcttt
    48361 cgccgcttcc tgccagctgt tttcgtcttt cgcgtggata actgccagcg gacgctgacc
    48421 gtctacctgg tcgcccagac gcgccatatc agtaaagccg acgctgtaat cgatggtgtc
    48481 agatgcctga cggcgtccgc cgcccattgc aaccactgcc atccccagtg cgcgggtatc
    48541 catttcactg acaaaccctt cggtatcagc atagactgct ttcgtcagca tcgctgtcgg
    48601 cagatacttc gcgtagttct caacgaagtc ggtcgggcct ttttgtgccg cgaccatacg
    48661 accaaagact tctgccgctt taccgttgtc cagcaccgcc tgcaatttcg cgcgcgcttc
    48721 ggcgtcattt ttcgccagtt tgccggagat cagcatctcc acgcacagtg ccatcgtgac
    48781 atcaaacaga cgcgggttac gatattcacc cgtcaggaac tgcaccgctt cacgaacttc
    48841 aaccgcgtta cctgcactgg aggccagtac ctgattcata tcggtgagca gcgcggtggt
    48901 gcgcacgcca gcgccgttag ccacgccaac aatcgcttcg gcaagggctt cagagagttc
    48961 gtaggtcggc ataaacgcgc cgctacctac tttcacgtcc atcaccagcg cgtccagacc
    49021 ttccgccagt ttcttcgcca gaatagaggc agtgatcagc gggatggagt ccacggttgc
    49081 ggtaatatca cgggtcgcgt agaaacgttt atccgccgga gccagtgagc tggtctggcc
    49141 gataatcgcc acgccgacgt ctttaataat ttcgcggaaa cggttgtcat ccgggaaaat
    49201 gtcgaagcca gggatggatt ccagtttgtc gagcgtaccg ccagtatgac cgaggccgcg
    49261 accggagatc atcggaatat agccgccgca ggctgcgacc atcggcccca acatcagcga
    49321 agtgacatcg ccgacgccgc cggtcgagtg tttatcaaca atcgggccat tcagatgcag
    49381 gcttttccag tcgagaacgg ttcctgaatc tcgcatagcc atggtcagcg agacacgctc
    49441 aggcattgtc atatcgtgga agaaaatggt catcgcgagg gcggcaatct gcccttcgga
    49501 gatagtgttg tcgcgaatac cgttgataaa gaaacgaatt tcttcatcgc tcagcgcatg
    49561 accatcacgt tttttacgaa taatttcttg tgcgagaaac aaggtaacct cccgtggaaa
    49621 agagacgagg taatcgggga accggatgag catcgctgca tcatccggca aatcaagcct
    49681 tagtagctgc tggcgctctt accgtcgccg tggcccagag ctttcagcag gcttgccagc
    49741 aggctggaag caccaaagcg gtagtgacgc gcatcagccc agtcagcacc gaacagttca
    49801 tcggcgatgg cgagatattt ctgcgcatct tccgcagtac gcacgccgcc cgccggtttg
    49861 aaaccaacgg ttttttctac gcccatatca cggatcactt ccatcatgat gcgcgcgctt
    49921 tccggcgtcg cgttcacagc cactttaccg gtagaggttt tgatgaagtc cgcacccgct
    49981 ttgatggaga tttcagacgc tttacggatc agcgcttcgt cttttagttc gccggtttcg
    50041 atgatcactt tcagcagtac attcgctgcc gcgcaagcct ctttacaggc tttcaccagg
    50101 tcaaaaccaa cctgctcgtt acccgccatc agcgcgcggt acgggaacac aacgtcaact
    50161 tcatcagcac cgtaggcgat tgccgcacgg gtttctgcca gcgcgatttc gatgtcgtcg
    50221 ttaccgtgtg ggaagttggt taccgtagcg atacggattt ccggggtacc ctgctctttc
    50281 agcgttttgc gagcaattgg gataaagcga ggatagatac agatagcggc ggtattgccg
    50341 accggagttt tggcctgatg acacagagca attactttct cgtcggtgtc gtcgtcattc
    50401 agggtggtca ggtccatcaa tttcagtgca cgcaggctgc ttgctttcag atcagtcatt
    50461 tcattctcct ggcttgtcgc caataaaatt caccttgcga gtttgttagt attctaacat
    50521 ctactccgca acacacttcg atatacatca caattaagga aatctactta caagtttgca
    50581 tcactgtaat gcgatctggt tcaaataatt cactttcaaa tgaatgcgtc agtggtggca
    50641 aacgcatcag gatcaaagtg aacatcacga aacttcttac aatggcgcat caccttcggc
    50701 ataaaaggaa aagaagatgc ccacttctca tgaaaatgca ctgcaacaac gttgccagca
    50761 aattgtcacc agcccggtac ttagcccgga gcagaagcgc cattttctgg cactggaagc
    50821 agaaaacaat ctgccttacc cacagcttcc tgccgaagcc cgccgcgcgc tggatgaagg
    50881 tgtaatctgc gatatgtttg aaggtcatgc gccgtacaaa ccgcgctatg tcttacccga
    50941 ttacgcccgt tttctggcga acggttccga atggctggag ctggaaggcg cgaaagatct
    51001 tgatgatgca ctctctctgc tgaccattct ttaccaccac gtaccatcgg tcacatcgat
    51061 gccggtctac ctggggcaac tggatgcgtt gttgcaaccg tatgttagaa ttctaacaca
    51121 agacgagatc gatattcgaa taaaacgttt ctggcgttac ctcgacagaa ccctgccaga
    51181 cgcctttatg cacgccaata tcggcccgtc tgattcgccc attacccgcg cgatcttacg
    51241 tgcagacgca gagttgaagc aggtttcacc gaacctgacc tttatctacg atcctgaaat
    51301 cacccctgat gacctactgc tggaagtggc gaagaacatc tgtgaatgta gcaaaccgca
    51361 catcgccaac ggtccggtgc atgataaaat tttcacaaaa gggggctacg ggattgtgag
    51421 ctgttacaac tcactgccgc tggcgggtgg tggcagcacg ctggtacgcc ttaacctgaa
    51481 agccattgcc gagcgcagtg aatcgctgga tgacttcttt acacgcactc taccgcacta
    51541 ctgccagcag cagatcgcca tcatcgatgc gcggtgtgaa ttcctctatc aacaatcaca
    51601 cttctttgag aatagcttcc tggtgaaaga agggctgatt aaccctgaac gttttgtgcc
    51661 aatgtttggc atgtacggat tggcggaagc agttaacttg ctgtgtgaga aagaagggat
    51721 tgccgcgcgc tacggtaaag aagccgccgc aaatgaagtg ggttatcgca tcagcgcaca
    51781 actggcggag tttgtcgcca atacccccgt gaaatatggc tggcaaaaac gcgccatgct
    51841 acacgcacag tcggggatca gttccgatat cggcaccacg cctggcgcac gcttgccgta
    51901 tggtgatgag ccagatccga ttacccatct gcaaactgtc gccccgcatc atgcttatta
    51961 ttattccggc atcagcgaca ttctgacgct cgacgaaacc atcaaacgta atccgcaggc
    52021 gctggtacag ctttgcctcg gtgcctttaa agccggaatg cgtgaattta ccgccaatgt
    52081 cagcggtaac gatctggttc gcgttaccgg ttatatggtg cgtttgtcgg atttagaaaa
    52141 atatcgcgcc gaaggttcac gcaccaacac cacctggctg ggagaagaag ccgcacgcaa
    52201 cactcgtatt ctggaacgcc agccgcgcgt gataagccat gaacagcaga tgcgctttag
    52261 tcagtaagat tatccccttc tcctgcgttg acgggccagg cagtcgtctg gctctgtttt
    52321 tgcagggctg caatctgcgc tgcaaaaact gtcacaatcc gtggacgatg gggcgctgta
    52381 atgattgcgg agagtgcgtg ccgcagtgtc cgcatcaggc gttgcagatt gtcgacggca
    52441 aagtggtgtg gaacgctgtg gtctgcgagc aatgtgatac ctgcctgaag atgtgtccgc
    52501 aacatgccac gccaatggcg cagtccatga gcgtggatga ggtgcttagc catatccgca
    52561 aagcagtgct gtttatcgaa gggataacgg taagcggcgg cgaagccacg acccagctac
    52621 cgtttgtggt ggcgctgttt actgctatca aaaacgatcc gcaactgcgc cacctcacct
    52681 gtctggtgga cagtaatggc atgttgagcg aaaccggctg ggaaaaattg cttccggtgt
    52741 gcgacggcgc aatgctcgat ctcaaagcgt gggggagcga atgtcatcag caattaaccg
    52801 gacgcgataa tcagcagatc aagcgcagca tctgtttgct ggcagagcgc ggtaagctgg
    52861 cggaactgcg tttgctggta atccctggcc aggtggatta tttgcaacac atcgaagaac
    52921 tggcggcgtt aatcaaggga cttggcgatg ttccggtacg cctgaacgcg tttcatgccc
    52981 acggcgtgta tggcgaggcg caaagctggc cgagcgccac gccggaggat gtggaacaac
    53041 tggctgatgc gttatgggag cgcggggtga gccggttaat atttccggcg ctatatttgt
    53101 gacatgccgg atacggccta cggatcggca cagacctgta ggcatgataa gatgcgtcaa
    53161 gcgtcgcatc aggcattgtg cgccagctgc cggatgcggc gtgaacgcct tatccggcct
    53221 acggttcggc acagacttgt aggcatgata agacgcgtca agcgtcgcat caggcattgt
    53281 gcgccaactg ccggatgcgg cgtgaacgcc ttatccggcc tacggcacgt taaacaacgt
    53341 atacgtatta tttagcaaca cttcggcaat ctcatcttct ggttccgggc gtaactcgca
    53401 aagtacgtca aacacccgcg cagcctgttc cgggcggtta ggctgcccct gaaagccgtt
    53461 gagcggcata tccggcgcgt cggtttccag caacaacgat gccagcggta attttgcgat
    53521 gacatcgcgg gttttactgg cgcgtggata ggtgatagta ccgcctacgc cgattttgta
    53581 gcccagcagt acaaaccttt cggcctgttg caggctgccg gaaaaaccgt gaaccacgcc
    53641 agtgcgtggc aaatcgtggc gtttaagatg catcgccagt ttgtcgtgtg tgcgccgtga
    53701 atgcaggatc accggcagat cgtagcgttt cgccagtttc agttgttcgt cgagtaacca
    53761 ctgctgcctc tcaaattgcg gatcgtcgcc gaagagatcc agaccgatct cccccaccgc
    53821 caccaccttc gccggacgcc tttccagcgc ctgctgtagc tgatcaagag acacatcgct
    53881 atgttttttc aacataccag gatgcaagcc caatgcggca tacagcggtt gatatttttc
    53941 cgctaacgcc tgcacgcggg caaaattttc cgcctctgtc gccggaacaa tgatcttgcc
    54001 tacgcccgct tgtgccgcgc gttgcaggct ggcctcttca tcaccactaa aaggcgggaa
    54061 atcaaaatgg cagtgggtgt cgataaaacg gcaaatcaag ccagatcctc attgttaaat
    54121 gtcgggtcgt tcgcctgcgg agcgtcgatc acttcggcaa ccagcgtatc gttggcgact
    54181 ggcgcaggcg gaatgacaat cgattccggc gtcaccaccg gcaccagatg gcgggtaagc
    54241 ggcgcttttt cagtcaataa cttaccgacc gttgccagaa aataacgacc gcataaacgc
    54301 ccaagtttat agtcttcacg cagcgccgga atccgactgc caagcgcgat actatgcaac
    54361 ggcttcggcg gataaatttc gaatatccgc agctggcccg gtggtttctc aataaattgc
    54421 tgaatgtcac gatagctggt ttcatgatgc tgcaccagat tgaccagcgg ctgcaggcta
    54481 ctgtcaccca gccagcgttc catgcgtttg aaccactgcg gcgtgtagta catttgcgac
    54541 ggcacggtgc ggatgacgac caaggttttt gctccctgcc ttgccgcctc tttaaccgga
    54601 atcgcatcac tgatcccgcc atccaggtag ttaatgcctt ccagcgacac tccgctacga
    54661 taaaagccag gtatcgcact ggaggcgcga atcacatcca gccagttttg tttggttggt
    54721 aaaaagtaat tcggcgcata gtcatcctga cgacaggcgc acatataaaa cgatttgccg
    54781 ctgtcaaaca accgcgcggc ggtatccatt tgcaacggca tctggcttgc agtggcctcc
    54841 accagccagt cgagatcgat aagatttcct ccacggacaa agcgcaatgg atcgaaaaat
    54901 tcgcgttttg tggtatagcg catgatgact ttgcgcgcgt aaccgggctg attgcagata
    54961 tacgccgaga ggttctgcgc cccggcagat gtgccgagat aaagatcgaa aggattaaac
    55021 tgcgcgcgca taaactcatc cagcacgcca gccgtgaaaa ttccacgctg tccgccgcct
    55081 tcgcacacca gagctattcg tccaggctgg aacggcctta gcgacaacgg cgcaatatta
    55141 ccaagcgtta caggtattcg ctgccccacc tctgccttcc tgtttttgtt gtgaaagaat
    55201 gacaaagtaa cgcaatttat tattcgctaa aaccgcaaaa gccagtccgc tggactggct
    55261 ttggataaaa ttttcttaat ggtctgaaat ctagggtcgt tttcggccca tgaacaaact
    55321 caccaggaac agaataatcc cgacgacaaa gacaatttta gctgcgcctg cagcggtacc
    55381 ggccagacca ccaaacccaa gtgcggcggc gattaacgcg ataaccagaa atatgatgcc
    55441 ccaacgaaac ataagcgtct cctttaccat agttaatgtc accgcttgat atgagcgtga
    55501 aaatcactca atgcgatatt tttagtgtgg tgcacatcgc gcctcccgac aaagttcggg
    55561 aggacgaatt acgacgaatt acttagtttt cagatcattt ttaacgcttt tcacaccatc
    55621 taccgctttg gcgatacttt cagcacggtc gctctgtgcc tgggaatcga cggtaccgga
    55681 gagctgaacc acgccgtcgg tggtttcaac tttcacatga cgggaaggga cgatatcgtc
    55741 cgccagcagt ttggctttga tttcactggt ggtggcggtg tcacccgcgt agcccttcac
    55801 cgagccttct ttagcgtcgc gaacgtgcag tttgtcgctg acagaggtca ccccttcaac
    55861 gcctttcgcc actttcactg cctcttcggc ctgagcctgg ctttcaacga aaccgctcag
    55921 ggtcacgact ttttgatcgg ttttgacaga gatatcggtg ctcttgatgt tgtcatgatc
    55981 caccagcgcc gccttcactt tcgcggtgat ggcgctgtca tccatgaaat taccgacttt
    56041 attcatagag ctatcgactt tttgccctgc gctttcattg gtagtctgtg cgttgttttc
    56101 cgcgtaggca gagccggtcg cgacggcaga ggtcaacatt acagccagca gagttttcga
    56161 aatcttcagt cttgtcatag tcatcgattt attcctgtat gtttgctcgt aatttgagcc
    56221 tggcaacacg aggttgcatt gctgaatagg gagagacttc accctctaca gaagtcaatg
    56281 gccgccatca caaaagcgat gggtgatgaa taacgaccat tacagcctct gaatcagtta
    56341 ttaatatcgg tagaatgaca atcgacggct ttagatactg atatctacgc attgaacggt
    56401 atttaacgcc gtcagaaatg tcattacttt gttaaatata gatcacaatt ttgaaaccgc
    56461 tcgggatatc agcgaaaaca taagcaaaag tgaatgtttt aagaacattc cgtaagcggc
    56521 taataaggaa gggaaattga cagggcgcag cggctaccgc gccctggagg caagaattaa
    56581 tgctcgcggg tctggtggaa ctgaacgtct ggataacgtt cctgcgccag gcgcaggttg
    56641 accatgctgg tagcgatgta agcgaggtta tcgccgccat caagcgccag ttggctttcg
    56701 ttcttacgct tgaactcttc gaatttcttc gcgtccgcac attctaccca gcgggcagtg
    56761 gcaacgttga ctgactcata cactgcttca acgttgtatt cgctcttcag acgcgctacc
    56821 accacatcaa actgcagcac accaaccgca ccaacgatca gatcgttgtt ggagatcgga
    56881 cggaacacct gcaccgcgcc ctcttcggaa agctgtacca gccctttaag cagctgtttt
    56941 tgcttcagcg gatctttcag gcggatacga cggaacagtt ctggcgcgaa gttcggaata
    57001 ccggtgaact tcatcatctc accctgggta aaggtgtcgc cgatctgaat ggtgccgtgg
    57061 ttgtgcagac caaggatatc gccaggatac gcttcttcaa cgtgcgaacg gtcacccgcc
    57121 ataaaggtca gcgcgtcgga gatcaccaca tctttcgcag tacgcacctg gcgcagcttc
    57181 atgccttttt catatttacc ggacaccaca cgcataaacg ccacgcggtc gcggtgtttc
    57241 gggtccatgt tggcctgaat tttaaatacg aagccggtaa atttgtcttc gctcgcctct
    57301 acggtacggg tatcagtctg acgcggcatc ggcgcaggag cccactccac caggccatcc
    57361 agcatatgat caacgccgaa gttacccagc gcagtaccga agaataccgg agtgatttcg
    57421 cccgcaagga acagctcttt gtcgaactcg ttagacgcgc ctttcaccag ctccagttcg
    57481 tcacgtagct gctgtgccag atcttcacca accgcagcgt cgagatccgg gttattcagc
    57541 cctttaacaa tgcggacttc ctgaatcgtg tggcctttac cgctctgata gagataggtt
    57601 tcatctttat aaaggtggta aacgccttta aacagcttgc cgcagccaat cggccaggtg
    57661 atcggtgcgc agccgatttt cagctcgttc tcaacttcat cgagcagctc catcgggtcg
    57721 cggatatcac ggtcaagttt gttcataaag gtgaggatcg gcgtgtcgcg cagacgggta
    57781 acttccatca gcttacgggt acgatcttca acaccttttg cggcgtcgat aaccatcagg
    57841 cagcagtcca ccgccgtcag ggtacgatag gtatcttccg agaagtcttc gtgccccggg
    57901 gtgtcgagca ggttaaccag gcaatcgtga tacggaaact gcatcacaga cgtagtaatg
    57961 gagatcccac gctgcttttc catctccatc cagtccgact tagcgtgctg gttggaacca
    58021 cggcctttta ctgtaccggc gctctgaatg gcctgtccga acagcagcac cttctcggtg
    58081 atggtagtct taccggcgtc cgggtgagaa ataatggcaa aagtgcggcg cttcgccacc
    58141 tcttgcaaat aaggagacaa cgtcataatt ctttcttctt gagtaagcgg catcgcgccg
    58201 cgcttgttga aaacgaaaaa ttgcggctat tttacccatc aacggggggg aggcaatcag
    58261 tgtttacaca ggagctgctc cagttcgtgc aacgaagaaa cggtccaggt gggcgcgatg
    58321 ccttctggtt gctcgcgatt gtgcgcattc agccagcagg tcgcaagccc ggcgttgatg
    58381 ccaccgagaa tatcggactc agctgtgtcg ccaaccatca gcacacgtga acggtcagga
    58441 ttgcctgcct gttccagcgc ataatcgaaa attttcttat tcggtttggc aacgccaact
    58501 tcttcggaaa tcaccagcaa atcgaaataa tcacgcaggc ccgtgcgttc cagacgcact
    58561 tgctgcaagg cgctaaagcc gttggtgatg atgccaattt tagcgttgcc acgaatggcg
    58621 ttaagcagag aaaccgcgcc cggcagtggc gtacagattt ccgccatcgc attaataaag
    58681 gcttcgttga gtttacccgg ctcgacgttc agccgttcgg cccagctctc aaaccgcccg
    58741 tgctgaagct gtaatgaagt gatcgcgccg ttttgataat ccacccacag tggcttgtta
    58801 acggcctgat agtcctgaaa atcttcagcg gtaaaggtga cgctgtaatc aagaaacatc
    58861 cgctgcaagc cggtgaatga gtcaaaggta aacagcgttt catcggcatc aaagaaaatc
    58921 cagtcccact tcattattcc accttgtctt acatactgat tggcaacgcc atgatgatgg
    58981 cgtcttcgcg accgtccgtg gtggggtagt aattgcggcg aattgtcgcc tcgttaaagc
    59041 ctaaactttc gtacagggca atggcggcag cgtttgaagc acggacttcc agccatagcg
    59101 tcgccacgcc gcgtttttcc agttcgtcga tcagatgttc cagcagcgcc cttcccaatc
    59161 cctgacgctg atagtcagga tcgaccgcaa tattgaacaa tgtagcttca tccagcacca
    59221 cttgcgtaat cgcaaacgcc gccattttgc cgttttgcgt taactgaaag ttgagataac
    59281 gctcgccctg gttgctggca aaggtttttt cactccacgg aaaggcgtgg gcgcgttgtt
    59341 caatgtggta agccgctggt aaatcagtcg tttcgaggga agaaatcgtg ttcatatgtg
    59401 caaatttgtt gccataacgc ggcgcgtgcc gttgggtttg cccgtaattc ggtgagcgcc
    59461 ggtgatgcca cctgagcgcc ttccagtgat agcggttcgt cagtacccaa ccgccagctg
    59521 ttgcagcgac tgccttgcgg cagcatcgcg attttttctg gcgtcagttg cagcacttga
    59581 tcggggctga cggttaacgc gcgcagaaca tcgctcacta taggatcggt cagggcggga
    59641 agatcgtttg ccaccatcac cagacggacg tgtgccggga tcgcaatggc aatctccccc
    59701 tgcaacgcgc cagggcgacg cagcgaccac tgggtaatgc ccagttgctg taactgccag
    59761 tctcgtcggg atgtcatagc gaatcgctcc tgttgtcagg ggcgcaaata tagcaaattc
    59821 gtcgataccg cgccaacata tggctataat cgccgccagt attaattgag gagcattcca
    59881 tgtctgcatt taccccggca agtgaagtct tgctgcgtca cagtgatgat ttcgaacaaa
    59941 gccgtattct ttttgccgga gacttacagg atgacctgcc cgcgcgttta gataccgcgg
    60001 ccagccgtgc tcatacccag caattccacc actggcaggt gttaagccgc cagatggggg
    60061 ataacgcccg ttttagtctg gtcgccacgg cggatgatgt cgcagattgc gatacgctga
    60121 tttactactg gccgaagaac aaaccggaag cccagttcca gttgatgaat ttactttctc
    60181 tgctgccagt gggcacggat atttttgtcg ttggcgagaa ccgcagcggc gtgcgcagcg
    60241 ccgagcagat gctgtcagat tatgcgccgt tgaataaaat ggacagcgct cgtcgctgtg
    60301 gcctctattt tggtcgtctg gaaaaacagc cagtatttga tgccgataaa ttctggggcg
    60361 aatacagcgt cgatggcctg acggtcaaaa cgctgcctgg cgtgtttagc cgcgacggtc
    60421 tggatgtcgg tagccagttg ctgctctcga cgttaacccc gcacacgaaa ggtaaagtgc
    60481 tggatgtcgg ctgtggcgcg ggcgtacttt cggttgcctt tgcgcgccat tcgccgaaaa
    60541 ttcgtctcac tctgtgcgat gtctctgcac cagcggtaga agccagccgc gcaacacttg
    60601 cggccaacgg tgttgaaggt gaagtctttg ccagtaacgt cttttccgag gtgaaaggtc
    60661 gttttgatat gatcatctcc aacccgccgt tccacgatgg gatgcaaacc agcctggatg
    60721 ccgcgcaaac gctgattcgc ggcgcggtgc gtcatcttaa tagcggcggc gagctgcgaa
    60781 ttgtagcgaa cgccttcctg ccttatccgg acgtgctgga tgagacattt ggcttccacg
    60841 aagtgatcgc gcaaaccggg cgcttcaagg tgtatcgcgc cattatgacc cgccaggcga
    60901 agaaaggttg agtatctgat atatgtcgcc gcataccgtg tcggatgcgg cgtacacgcc
    60961 ttatctgacc gacaaacgaa ttatccacaa tccgctgatg gacatttttc cagcaattac
    61021 acctctgtcg ataattaact attgacgaaa agctgaaaac cactagaatg cgcctccgtg
    61081 gtagcaattc tttttaagaa ttgacggtat gcgaaggtgg cggaattggt agacgcgcta
    61141 gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct cgcaccaaaa
    61201 accacgttga tattgctcgc actgggcgaa ggtggcggaa ttggtagacg cgctagcttc
    61261 aggtgttagt gttcttacgg acgtgggggt tcaagtcccc cccctcgcac caaacgaggc
    61321 aatatcaaaa aaagtaagat gactgtgcga aggtggcgga attggtagac gcgctagctt
    61381 caggtgttag tgtccttacg gacgtggggg ttcaagtccc ccccctcgca ccaattatct
    61441 ttacttcctt tcttgtttct tccctgtttt atcatccctg cttcaaattc attatcatca
    61501 gcatgatgcc agcaataagc gccaggcacg atggcagcaa aacgtaatgc cgcaactgtt
    61561 tgtgatacag catcgccggc gtcaatacta atcccatcag aatgataatc cgccattgat
    61621 ccggcgataa atccgcatac atcagccctg caatcagcaa tcccggcagc aacactcccc
    61681 agccactgcc cagcgtacgt tgcaacatgg tttcatctct ttcattgata atgataacca
    61741 atatcatatg ataattttta tcatttgcaa gccagagaaa tcccttgcta tcgggtaaac
    61801 ctatcgctag gattagcaat cattatcatt tagattacta tcacgattat ggcctatcgt
    61861 tccgcaccgc tctatgaaga tgttatctgg cgaacgcatc tccagccgca ggatgctggg
    61921 cttgcacaag ccgtacgggc gacgatcgcc gaacatcgtg aacatttgct ggagtttatc
    61981 cgtctggatg aacccgcacc gctccgtgcc atgacgctgg cgcaatggtc atcaccgaat
    62041 acgctaagtt ctcaactggc ggtctattcc gatcatatct accgcaacca acccttgatg
    62101 atccgcgaga acaaaccgct gatctcacta tgggcacaat ggtatatcgg cctgatggtg
    62161 ccaccattaa tgttggcgct gctgacgcag gaaaaagcat tagatgtgtc gccggaacat
    62221 ttccacgttg agtttcacga aaccggacgc gccgcctgtt tctgggtcga tgtgtgtgaa
    62281 gataaaaacg caacactaca ttcgccgcag cagcgaatgg aaacgttaat cagccaggcg
    62341 cttgttccgg tagtgcaggc attagaagcg acgggagaga tcaacggcaa acttatctgg
    62401 agcaataccg gttatttgat caactggtat ctgactgaga tgaaacagct gcttggcgag
    62461 gcgacggttg aatcgctgcg ccatgccctc ttttttgaga aaatgctcac gaacggtgaa
    62521 gataatccac tctggcgtac cgtggtactg cgcgacggcc tgctggtgcg ccgcacttgc
    62581 tgccagcgtt atcgcttacc ggatgtgcag caatgtggcg attgtacgct gaaatgaagc
    62641 actgccgggg aagtaaaccc ggcatcatgt ggattaatag ggatgcaaca cattacttgc
    62701 ttcgcaatgc cgcatacata acagaatatc tgcggcctcc aacaaccctg catcagaact
    62761 gacgcccagt ttcgacatca cattaaattt atgtgcacgg atcgttttga tattgcgttt
    62821 aagctgctcg gcaatttgtg tcattgagta gccacgcgac ataaagcgca atatttctct
    62881 ctccgtcggg cttagcgttc ggctttgatt aatgtaccac tgattgttca gtcggtcggt
    62941 cgcctgacgt acaccattta atgacaaaaa gagttcctga tgaaaaatct ccagcgttga
    63001 cgctttactt aatacaccgt ccaacggtga tggcgacaat gaaccaatca gccgagcttc
    63061 aatatcatca tccgcaataa ctaaacgccg ggtacgcgga aacttaatcg ccagttcagt
    63121 caggcaagat aatccctctc tgcgctcact tctcatggca gaaaaagaaa aaatgaccgc
    63181 cgcaaacgag atccgcgaca tggcatcctg aaatgactcc tgactggtat aggtatggag
    63241 cttataaggg ttacccgcaa actttctgaa taaactctca ataccgatac tgctcatgac
    63301 gcatttttct atgagtgcga catttctctt cttaattcgg ctgtgttcca ttctgcggca
    63361 tctccataaa cagaaggcaa gttaagttct cttgcaccct gcgcgctatt tatccatgca
    63421 tacatttcag cgttgctgtg aattgccagt cgacgcatcg cactgttttt ttgggcgctg
    63481 atagttttat tacttttctt aagcaatgaa gctatctggt tgatgcccca tcctttacct
    63541 aatagccgca gtaccttgcg ctctgaaaga gtgagaatga cggagcgata gctaaaatca
    63601 tgagtttcag tcattgccgg agaggtcagc atggcgctga tacgctctgc gtgcgtattg
    63661 ccggaacgta cggtcttgac cagattctca atgggttcaa catcagacaa taacgtcgcc
    63721 gtagggcaca taagcagttc tactgcctgg gaataccagg agcgtgacac catgaaaacc
    63781 cagtgaattt cccgatattg tgagattaaa gaataataat gttcacaaac agaacgtgga
    63841 tcttcacttt caccggctaa atcagcaatg actaatccgg aacgacgtaa ttgtaataag
    63901 gtaaggtcct ccgcagaggc gctggatatt atttcatatt cagggaagtg agtcctcatg
    63961 acctctttta accctgcttg cataacaggt attttactga taacaattcc atttttgcag
    64021 catcctggca acatatttcc tccacatcca ctgtctaaaa atcatgatgg tttattcagg
    64081 atattcattc cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag
    64141 cattcacttt catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag
    64201 gttagcatca aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa
    64261 gttgttagaa atacttaatg aa
//
LOCUS       DAZKJR010000009        63941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKJR010000009 DAZKJR010000000
VERSION     DAZKJR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.009"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..271)
                     /gene="yfhL"
                     /locus_tag="ACHOWS_000590"
     CDS             complement(11..271)
                     /gene="yfhL"
                     /locus_tag="ACHOWS_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFQ0046228.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(327..1175)
                     /locus_tag="ACHOWS_000591"
     CDS             complement(327..1175)
                     /locus_tag="ACHOWS_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0046229.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            1384..2019
                     /gene="yfhb"
                     /locus_tag="ACHOWS_000592"
     CDS             1384..2019
                     /gene="yfhb"
                     /locus_tag="ACHOWS_000592"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFQ0046230.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2077..2580
                     /gene="tadA"
                     /locus_tag="ACHOWS_000593"
     CDS             2077..2580
                     /gene="tadA"
                     /locus_tag="ACHOWS_000593"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0046231.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2577..4133)
                     /gene="mltF"
                     /locus_tag="ACHOWS_000594"
     CDS             complement(2577..4133)
                     /gene="mltF"
                     /locus_tag="ACHOWS_000594"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFQ0046232.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            4391..8278
                     /gene="purL"
                     /locus_tag="ACHOWS_000595"
                     /gene_synonym="purI"
     CDS             4391..8278
                     /gene="purL"
                     /locus_tag="ACHOWS_000595"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFQ0046233.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGASGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8835..10262
                     /gene="qseE"
                     /locus_tag="ACHOWS_000596"
     CDS             8835..10262
                     /gene="qseE"
                     /locus_tag="ACHOWS_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFQ0046234.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIDLPSSKNTK"
     gene            10427..11140
                     /gene="qseG"
                     /locus_tag="ACHOWS_000597"
     CDS             10427..11140
                     /gene="qseG"
                     /locus_tag="ACHOWS_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFQ0046235.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            11130..12464
                     /gene="glrR"
                     /locus_tag="ACHOWS_000598"
     CDS             11130..12464
                     /gene="glrR"
                     /locus_tag="ACHOWS_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFQ0046236.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            12525..12863
                     /gene="glnB"
                     /locus_tag="ACHOWS_000599"
     CDS             12525..12863
                     /gene="glnB"
                     /locus_tag="ACHOWS_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFQ0046237.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(12908..14098)
                     /gene="hmpA"
                     /locus_tag="ACHOWS_000600"
     CDS             complement(12908..14098)
                     /gene="hmpA"
                     /locus_tag="ACHOWS_000600"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFQ0046238.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            14427..15680
                     /gene="glyA"
                     /locus_tag="ACHOWS_000601"
     CDS             14427..15680
                     /gene="glyA"
                     /locus_tag="ACHOWS_000601"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0046239.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(15743..16936)
                     /locus_tag="ACHOWS_000602"
     CDS             complement(15743..16936)
                     /locus_tag="ACHOWS_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0046240.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGCLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            17054..20335
                     /locus_tag="ACHOWS_000603"
     CDS             17054..20335
                     /locus_tag="ACHOWS_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFQ0046241.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNITLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKCSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            20432..21415
                     /locus_tag="ACHOWS_000604"
     CDS             20432..21415
                     /locus_tag="ACHOWS_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0046242.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            21438..22949
                     /locus_tag="ACHOWS_000605"
     CDS             21438..22949
                     /locus_tag="ACHOWS_000605"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFQ0046243.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKDAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            22974..23972
                     /locus_tag="ACHOWS_000606"
     CDS             22974..23972
                     /locus_tag="ACHOWS_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0046244.1"
                     /translation="MSASSLLLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24005..25099
                     /locus_tag="ACHOWS_000607"
     CDS             24005..25099
                     /locus_tag="ACHOWS_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFQ0046245.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            25111..25983
                     /locus_tag="ACHOWS_000608"
     CDS             25111..25983
                     /locus_tag="ACHOWS_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFQ0046246.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDILC
                     LVYEHRSGVYHYRVSQTFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(26132..26554)
                     /locus_tag="ACHOWS_000609"
     CDS             complement(26132..26554)
                     /locus_tag="ACHOWS_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFQ0046247.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(26651..27853)
                     /gene="hcaD"
                     /locus_tag="ACHOWS_000610"
     CDS             complement(26651..27853)
                     /gene="hcaD"
                     /locus_tag="ACHOWS_000610"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFQ0046248.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRIDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLID
                     EDIALKSL"
     gene            complement(27863..28675)
                     /gene="hcaB"
                     /locus_tag="ACHOWS_000611"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(27863..28675)
                     /gene="hcaB"
                     /locus_tag="ACHOWS_000611"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFQ0046249.1"
                     /translation="MSDLHNESIFITGGGSGLGLALAERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(28672..28992)
                     /locus_tag="ACHOWS_000612"
     CDS             complement(28672..28992)
                     /locus_tag="ACHOWS_000612"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFQ0046250.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(28992..29510)
                     /gene="hcaF"
                     /locus_tag="ACHOWS_000613"
     CDS             complement(28992..29510)
                     /gene="hcaF"
                     /locus_tag="ACHOWS_000613"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFQ0046251.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            complement(29507..30868)
                     /gene="hcaE"
                     /locus_tag="ACHOWS_000614"
     CDS             complement(29507..30868)
                     /gene="hcaE"
                     /locus_tag="ACHOWS_000614"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFQ0046252.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKHGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            31004..31894
                     /gene="hcaR"
                     /locus_tag="ACHOWS_000615"
     CDS             31004..31894
                     /gene="hcaR"
                     /locus_tag="ACHOWS_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFQ0046253.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            32054..33193
                     /locus_tag="ACHOWS_000616"
     CDS             32054..33193
                     /locus_tag="ACHOWS_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFQ0046254.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSSLTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33185..34465)
                     /gene="csiE"
                     /locus_tag="ACHOWS_000617"
     CDS             complement(33185..34465)
                     /gene="csiE"
                     /locus_tag="ACHOWS_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFQ0046255.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(34656..35510)
                     /locus_tag="ACHOWS_000618"
     CDS             complement(34656..35510)
                     /locus_tag="ACHOWS_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0046256.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAEKAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(35655..36458)
                     /gene="suhB"
                     /locus_tag="ACHOWS_000619"
     CDS             complement(35655..36458)
                     /gene="suhB"
                     /locus_tag="ACHOWS_000619"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFQ0046257.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            36577..37317
                     /gene="trmJ"
                     /locus_tag="ACHOWS_000620"
     CDS             36577..37317
                     /gene="trmJ"
                     /locus_tag="ACHOWS_000620"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFQ0046258.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            37587..38075
                     /gene="iscR"
                     /locus_tag="ACHOWS_000621"
     CDS             37587..38075
                     /gene="iscR"
                     /locus_tag="ACHOWS_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFQ0046259.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            38187..39401
                     /gene="iscS"
                     /locus_tag="ACHOWS_000622"
     CDS             38187..39401
                     /gene="iscS"
                     /locus_tag="ACHOWS_000622"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0046260.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            39429..39815
                     /gene="iscU"
                     /locus_tag="ACHOWS_000623"
     CDS             39429..39815
                     /gene="iscU"
                     /locus_tag="ACHOWS_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFQ0046261.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            39832..40155
                     /gene="iscA"
                     /locus_tag="ACHOWS_000624"
     CDS             39832..40155
                     /gene="iscA"
                     /locus_tag="ACHOWS_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFQ0046262.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40251..40766
                     /gene="hscB"
                     /locus_tag="ACHOWS_000625"
     CDS             40251..40766
                     /gene="hscB"
                     /locus_tag="ACHOWS_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFQ0046263.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            40783..42633
                     /gene="hscA"
                     /locus_tag="ACHOWS_000626"
     CDS             40783..42633
                     /gene="hscA"
                     /locus_tag="ACHOWS_000626"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFQ0046264.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            42635..42970
                     /gene="fdx"
                     /locus_tag="ACHOWS_000627"
     CDS             42635..42970
                     /gene="fdx"
                     /locus_tag="ACHOWS_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFQ0046265.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            42982..43182
                     /gene="iscX"
                     /locus_tag="ACHOWS_000628"
     CDS             42982..43182
                     /gene="iscX"
                     /locus_tag="ACHOWS_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFQ0046266.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43241..44524
                     /gene="pepB"
                     /locus_tag="ACHOWS_000629"
     CDS             43241..44524
                     /gene="pepB"
                     /locus_tag="ACHOWS_000629"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFQ0046267.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            44643..45419
                     /gene="sseB"
                     /locus_tag="ACHOWS_000630"
     CDS             44643..45419
                     /gene="sseB"
                     /locus_tag="ACHOWS_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFQ0046268.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(45451..45576)
                     /gene="timP"
                     /locus_tag="ACHOWS_000631"
     CDS             complement(45451..45576)
                     /gene="timP"
                     /locus_tag="ACHOWS_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFQ0046269.1"
                     /translation="MKIRCFCIVLIVSGALLSEVNNNRSLSGNNLLAVNNLQSSK"
     gene            complement(46238..47083)
                     /gene="sseA"
                     /locus_tag="ACHOWS_000632"
     CDS             complement(46238..47083)
                     /gene="sseA"
                     /locus_tag="ACHOWS_000632"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFQ0046270.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            47290..52251
                     /gene="yfhM"
                     /locus_tag="ACHOWS_000633"
     CDS             47290..52251
                     /gene="yfhM"
                     /locus_tag="ACHOWS_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFQ0046271.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNSARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTTSGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFTLWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLSASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            52252..54564
                     /gene="pbpC"
                     /locus_tag="ACHOWS_000634"
     CDS             52252..54564
                     /gene="pbpC"
                     /locus_tag="ACHOWS_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFQ0046272.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLALLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAIPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLAEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            54713..55144
                     /gene="ndk"
                     /locus_tag="ACHOWS_000635"
     CDS             54713..55144
                     /gene="ndk"
                     /locus_tag="ACHOWS_000635"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0046273.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            55294..56448
                     /locus_tag="ACHOWS_000636"
     CDS             55294..56448
                     /locus_tag="ACHOWS_000636"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFQ0046274.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            56733..57740
                     /gene="rodZ"
                     /locus_tag="ACHOWS_000637"
     CDS             56733..57740
                     /gene="rodZ"
                     /locus_tag="ACHOWS_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFQ0046275.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            57767..58885
                     /gene="ispG"
                     /locus_tag="ACHOWS_000638"
                     /gene_synonym="gcpE"
     CDS             57767..58885
                     /gene="ispG"
                     /locus_tag="ACHOWS_000638"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFQ0046276.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            58996..60270
                     /gene="hisS"
                     /locus_tag="ACHOWS_000639"
     CDS             58996..60270
                     /gene="hisS"
                     /locus_tag="ACHOWS_000639"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0046277.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRTLGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            60288..60908
                     /gene="yfgM"
                     /locus_tag="ACHOWS_000640"
     CDS             60288..60908
                     /gene="yfgM"
                     /locus_tag="ACHOWS_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFQ0046278.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            60919..62097
                     /gene="bamB"
                     /locus_tag="ACHOWS_000641"
     CDS             60919..62097
                     /gene="bamB"
                     /locus_tag="ACHOWS_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFQ0046279.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            62215..63687
                     /gene="der"
                     /locus_tag="ACHOWS_000642"
     CDS             62215..63687
                     /gene="der"
                     /locus_tag="ACHOWS_000642"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0046280.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEADDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            63750..>63941
                     /locus_tag="ACHOWS_000643"
     CDS             63750..>63941
                     /locus_tag="ACHOWS_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFQ0046281.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKK"
BASE COUNT        15087 a        16061 c        17714 g        15079 t
ORIGIN      
        1 gggtagaata ttaaatttta tccgcgtgat gcataagcac aaatttatcc cacaactgtt
       61 cttccgtctc gacatgttgc ggatccttca caatagtatt ggggatcggg cacaccttct
      121 ggcaggttgg tgtctcgtag tgccctacgc attcggtaca cttatcgctg ttaatctcgt
      181 agatatgatc gcccattgaa atcgcctcat tcgggcattc gggttcacac atatcacaat
      241 tgatgcattt tttagtgatt aacagcgcca taaggaaact ctcaaaacaa cacaaaccgg
      301 gcgggcatta tacgcgcatt ctttactcag accagttttt tcaccagcgc ttcactgtga
      361 cgaatacgtt ctggagccag ttccagatcc tgctgaatca gcgcaatgaa cagcaaatcc
      421 gttaacattc cctgcgcgtg acaagcagag attgaagcac tgtttgtcgc ttgttcttcg
      481 gcaatggtat acaggcaatg agaagcacgc tgttgcaggg cattgggagt aaagccggta
      541 atcgccagca cttttccgcc cactcgcagc atctcatctg ccgccaggtt taactcgcgt
      601 cgtacaccgg tgtaggaaat ggctaataac agatcgtcag gggacgacgc ctgtactgta
      661 gcgagtagcg catgcatatc gcgcactgcg gcagcattga agccaatctt catcagcttc
      721 caggcaaagt tttgcgccac cagacccgaa gcgccaatac cggtcagaat tatccgccgc
      781 gcagagcgca acattgccac gcattcatgc agtttctctt cactattaac gtttaacgtt
      841 gcgtacatag cggcagtatt ttctttaatc agtttttcgc cgaccagccg taacggatca
      901 tcaccgcgga tttggttatg aatgggcacg gagggagatt ccggctggct tgccagcgct
      961 tcactcaacg ccaacttaag tgccggaaaa cctttatagc cgagtttttg cgcgaacttc
     1021 acgacgctgg actgactgac tccggcttcg ttggccagtt gctgagagct taaatggcgc
     1081 gccgtatcag gttgtagcag cagataatcc gccagttttt tatcactttg ggcaagcccc
     1141 tggtaacgct gacggatacg aagtaagccg ttcatatctt tcctcgcgat gaaatagtgg
     1201 cgacaacagg caatatgaga cacacgcgag tgaattttat attccattgc cgctaattgc
     1261 catgaattaa aaattccagt tagccatgat gcggtcgatt gttgccggaa aagtgatgat
     1321 ctatctgtta ggctatgtag tagcgaaaat taatcaggat gtttcagtcc agaggagtat
     1381 ggtttggcaa ctcacgagcg tcgtgtggtg ttttttgact tagatggaac attgcatcag
     1441 caggatatgt tcggcagttt tctgcgttat ttactgcgtc gccaaccgct gaatgcgtta
     1501 cttgtcctgc cgttgttgcc gatcatagcc attgcgttat tgataaaagg tcgtgcggca
     1561 cgctggccga tgagtctgct tctgtgggga tgcacttttg gtcacaatga agcgcgtttg
     1621 caggcgttgc aggccgattt cgtacgctgg tttcgtgaca atgttaccgc ctttccgctg
     1681 gttcaggagc gattaaccac ctacctgtta agttccgatg ctgatatctg gttgattacc
     1741 ggctctccgc agccgctggt tgaagcggtt tatttcgata cgccctggct gccgcgggtt
     1801 aatcttatcg ccagccaaat tcagcgtggc tatggtggtt gggtattgac gatgcgttgt
     1861 ctgggacatg aaaaggtcgc acaactggag cgcaaaatcg gcacgccgct gcggctgtac
     1921 agtggctata gcgacagtaa tcaggacaat ccgctgcttt atttctgtca gcatcgttgg
     1981 cgagtaaccc cgcgcggtga actccagcaa ctggaataga gtaaagcata gcgtccgtgt
     2041 ataatgcgcc gcgcttttat aaccggagtt ttctttttgt ctgaagtcga atttagccac
     2101 gaatactgga tgcgtcacgc gctgacgctg gcaaagcgtg cctgggatga gcgggaagtg
     2161 ccggtcggcg cggtattagt gcataacaat cgtgttattg gcgaaggctg gaaccgtccg
     2221 attggtcgac atgatcccac cgcacatgca gaaatcatgg ccctgcggca gggcggtctg
     2281 gtgatgcaaa attatcgact gatcgacgcc acgctgtatg tcacgcttga accctgtgta
     2341 atgtgtgccg gagcaatgat ccacagtcgc attggtcgcg tggtctttgg tgcgcgtgac
     2401 gcgaaaactg gcgctgcggg atctttaatg gatgtgctgc atcatccggg tatgaatcac
     2461 cgggtggaaa ttaccgaagg aatactggcg gatgagtgcg cggcgttgct cagtgacttc
     2521 tttcgcatgc gccgccagga aattaaagcg cagaaaaaag cgcaatcctc gacagattaa
     2581 ttttgtttct cttcactccc ttttctggaa aacagcagag agggagagtg ggtcaaatag
     2641 tttgatgacg actgggaaag aaacgagaga aaaggaaatt tctctttgcc cagctccgta
     2701 ggcgataccg ccggataatc ctgcgccagt tgcatcgccg cttctgtagc ctgcttctct
     2761 ttctcctgca gataacccac caggctaatc tgatacttac gaatattttc gacataagcg
     2821 taggcttcat gcccacgagc gtagccgtaa gtcagcttgc tgtaataggg tttctggcta
     2881 agtaaaggca gacgctgttt tacgtcagcc caactgtcag gattaccttt ggtttttgcc
     2941 gtcagggcgc gggcatccag catatgcgca tagcccatat tgtacgcggc aagggcaaac
     3001 cagatccgtt cgttctccgg cacactttcc ggcactttac tcatcatatc ctgcaaataa
     3061 cgcacgccgc cgctgatgct ctgttcggca tcggtacgat ccgtaatgcc gaggctttgc
     3121 gcggtgtttt tggttaacat catcatgccg cgcacacccg tcggtgaagt ggcctgagca
     3181 tcccagtgcg attcttgata agcaatagcg gccagcaaac gccagtcaat ttcttcggcg
     3241 tatttctcaa acaggggctt taactgcggc agtaccgcat cgacggcgcg taaaaatgtg
     3301 cgcgtatcga cgtaatcaaa atcatcgcca tgccccaggt atttctcttc aatgcgtgcc
     3361 agcgtaccgt cttcattcat ctcattgaag aaatcgagca gagcggcgga aagggtatta
     3421 tcgccatcta acgggctaaa ccaggtcacc ggttgttcat cggtgatatc gagcgctacg
     3481 gcgagttccg ggtgaacgcg ctgaaacagg ctgatggcga cagaatcagc aatggtgtaa
     3541 tcgagttttc cttcgatgac atcttccatt aattccgcag agcctttttt gtcgtctacc
     3601 ttccagctta attccgggaa ttttgtttct ttcagggtct ggagatcgtt aaccaccaca
     3661 tgacccggtg caacggtgag ttgctccgcc gtcaggttgc ccagtgtacg tgggcgatac
     3721 tgacccactt tataaaccag ttgttgtgac acggaataat aggtagggcc aggctgataa
     3781 ttttttacac gctcactgtt atagaccagg cctgccgcca gcaggtcggc gttaccatta
     3841 tcgaggtcgt caaacagctg gctgatattc tgccgcacgg tcactttcag ttttacgccg
     3901 aggtaatcgg caaactgttt cgccagttcg taatccaggc caaaaggttt cccgttgatt
     3961 tcgttataag tcaggggggt atgaatggtg ctcacacgca actctccccg cgcttgaatg
     4021 gcggcgatac ggttgtcggc tttaccaaac cagggaatgg atggccagag agcgaccgcg
     4081 agcagcagtg ccagaatgcc gatgaacaga taattaatct ttaatttttt caattagtta
     4141 attctctgtg tcgtgcgcgt cccagcttga aaaaacgtaa taatagtgaa aggtttactc
     4201 ataaatgagc ggcattttgc gtaaacctgc gccagttggc aacttattac agccattggc
     4261 ggcacgcgtt gctaattcac gatggtgatt ttatttccac gcaaacggtt tcgtcagcgc
     4321 atcagattct ttataatgac gcccgtttcc cccccttggg tacaccgaaa gcttagaaga
     4381 cgagagactt atgatggaaa ttctgcgtgg ttcgcctgca ttgtcggcat tccgaatcaa
     4441 caaactgctg gcacgttttc aggctgccag gctcccggtt cacactattt acgccgagta
     4501 tgtccatttt gctgacctca atgcgccgtt aaacgatgat gagcacgcac aacttgaacg
     4561 cctgctgaaa tatggcccgg cactcgccag ccacgccccg caaggcaaac tcctgctggt
     4621 gaccccgcgt cctggcacca tctctccctg gtcttcgaaa gcgaccgata tcgcccataa
     4681 ttgcggtcta caacaggtaa accgccttga gcgcggcgtt gcttactatg tagaagccgg
     4741 tacgctgacc aatgaacaat ggcagcaggt taccgctgaa ctgcacgacc gcatgatgga
     4801 aacggtcttt tttgctttag atgatgcaga gcagctgttt gcacaccatc aaccgactcc
     4861 ggtcaccagc gttgatttgc tggggcaggg ccgtcaggcg ctaatcgacg ctaacctgcg
     4921 tcttggcctg gcactggcgg aagatgaaat tgactatttg caggatgctt tcacaaagct
     4981 gggtcgtaac ccgaacgaca tcgaactgta tatgtttgcc caggcgaact ccgagcactg
     5041 ccgccacaaa atttttaacg ccgactgggt tatcgatggt gaacagcagc cgaaatcact
     5101 gtttaagatg atcaaaaaca ccttcgaaac cacgccggat cacgttctct ctgcttataa
     5161 agataacgcc gccgtaatgg aaggttctga agtgggccgc tactttgctg accacgaaac
     5221 gggccgctac gatttccatc aggaacctgc gcatatcctg atgaaagttg aaactcacaa
     5281 ccacccgacg gcgatttctc cgtggccggg cgcggcgacc ggttccggcg gtgaaatccg
     5341 cgatgaaggt gcctccgggc gcggcgcgaa gccgaaagct ggtctggtcg gtttctccgt
     5401 gtccaacctg cgaattcctg gcttcgaaca gccgtgggaa gaagatttcg gtaaacctga
     5461 gcgcattgtt accgcgctgg acatcatgac cgaaggcccg ctgggcggtg cggcgtttaa
     5521 caacgaattt ggtcgtccgg cactgaatgg ctacttccgt acttatgaag aaaaagtgaa
     5581 cagccacaat ggcgaagagc tgcgcggtta tcacaaaccg atcatgctgg cgggcgggat
     5641 cggcaacatt cgtgccgatc acgtacaaaa aggcgagatc aacgttggtg cgaagctggt
     5701 cgttctcggc ggcccggcaa tgaacatcgg tcttggcggc ggcgcggcgt cttccatggc
     5761 gtctggtcag tctgatgccg acctcgactt cgcttccgta cagcgcgaca acccggagat
     5821 ggagcgtcgc tgccaggaag tgatcgaccg ttgctggcag cttggtgatg ccaacccaat
     5881 cctgtttatc cacgacgttg gcgcaggcgg tctttctaac gccatgccgg aactggtgag
     5941 cgacggcggg cgcggcggta aattcgaact gcgcgatatc ctgagcgacg aaccgggcat
     6001 gagcccgctg gaaatctggt gtaacgaatc ccaggaacgc tacgtgctgg cagttgctgc
     6061 cgatcagtta ccgctgtttg acgaactgtg taagcgtgag cgcgcgccat acgcggtgat
     6121 tggtgaagcg accgaagaac tgcatctttc tttgcacgat cgtcattttg ataatcagcc
     6181 gatcgatctg ccgctggacg tactgcttgg taaaacgccg aagatgaccc gcgaagtaca
     6241 aacgctgaaa gcgaagggtg atgcgctggt tcgtgaaggg attaccattg ctgacgcggt
     6301 gaaacgcgtg ctgcacctgc cgaccgtggc ggagaaaact ttcctggtga ccattggtga
     6361 ccgcagcgta accggcatgg tggcgcgcga tcagatggtg ggtccgtggc aggtgccggt
     6421 cgctaactgc gcggtcacta ccgccagcct cgatagctac tacggtgaag cgatggcgat
     6481 tggcgagcgt gcgccggttg cgctgctgga tttcgccgct tctgcccgtc tggcggtcgg
     6541 tgaagcgtta accaacatcg ccgcaacaca aattggcgat atcaaacgca ttaaactttc
     6601 cgccaactgg atggcggcgg caggccaccc tggtgaagat gcaggcctgt atgaagccgt
     6661 taaagccgtg ggtgaagaac tttgtccggc gctgggcctg acgatcccgg tgggtaaaga
     6721 ctctatgtcg atgaaaaccc gttggcagga aggtaacgaa gagcgcgaaa tgacgtcgcc
     6781 gctgtcgctg gtgatttctg catttgcccg cgtggaagat gtacgtcaca ccatcacgcc
     6841 acagctttct accgaagata acgcactgct gctgattgat ttgggcaaag gcaataacgc
     6901 gctgggcgca acggcgctgg cgcaggttta tcgtcagctt ggcgacaaac cggcagatgt
     6961 acgcgatgtc gcgcaactga aaggcttcta tgacgcgatt caggcgctgg ttgcacagcg
     7021 taagctgctg gcgtatcacg accgctctga tggcggcctg ctggtaacgc tggcggaaat
     7081 ggcctttgct ggtcattgcg gcattgacgc ggatatcgcc tctctgggtg acgatcgcct
     7141 ggcggcgttg tttaacgaag aactgggtgc ggtgattcag gttcgtgccg ctgaccgtga
     7201 agccgttgaa gcggtactgg cacagcatgg gcttgcggat tgcgtccatt atgtagggca
     7261 ggcggtttcc ggtgaccgtt ttgtgattac cgccaacggg cagactgtat tcagcgaaag
     7321 ccgcactacg ttgcgtgtct ggtgggcaga aactacctgg cagatgcagc gcctgcgtga
     7381 caacccggag tgtgccgatc aggagcacca ggcgaaatca aacgacgccg atccgggcct
     7441 gaatgtgaaa ctgtcgttcg atatcaacga agatgtggca gcaccgtata ttgccactgg
     7501 tgcgcgtccg aaagttgccg tgctgcgtga gcagggggtg aactcgcatg ttgaaatggc
     7561 ggcagctttc caccgtgcag gcttcgatgc aatcgatgtg catatgagcg acctgctggc
     7621 tggacgcacg ggcctggagg atttccacgc tctggtagcg tgcggtggat tctcctacgg
     7681 tgacgtgctg ggtgctggtg aaggctgggc gaagtctatc ctgttcaatg accgtgtacg
     7741 cgatgagttt gcaaccttct tccaccgtcc gcaaacgctg gcgctggggg tatgtaacgg
     7801 ttgccagatg atgtctaacc tacgcgaact gatcccaggt agtgagttgt ggccacgttt
     7861 tgtgcgcaat acctccgatc gctttgaagc gcgtttcagc ctggttgaag taacccaaag
     7921 cccgtctctg ctgttgcagg ggatggtggg ctcgcaaatg ccgattgctg tctctcatgg
     7981 cgaagggcgc gtggaagtgc gtgatgcggc gcatctggcg gcactggaaa gcaaagggct
     8041 ggtggcactg cgctatgtcg ataacttcgg caaagtcact gaaacctacc cggctaaccc
     8101 gaacggttct ccgaatggta ttacggcagt cacgactgaa agtggtcgag tcaccattat
     8161 gatgccgcac ccggaacgtg ttttccgtac tgtcagcaac tcctggcatc cggaaaactg
     8221 gggcgaggat gggccgtgga tgcgtatttt ccgcaatgca cgtaagcagt tggggtaagc
     8281 cgtcaaccca ttggttttgc agccggatga ggcattaagc accaactact attataagct
     8341 cccaacttcg ggagcttttt tgtgtctgta aatcacgaca atgggtggtt taccgcgtcg
     8401 ctttctggcg acacttaact aattgatttt aatggcatct aataagttta tcttaagttg
     8461 tctcttttta gcgacacagt ggctgattta cagccaaata gcgaagaagc cataacccct
     8521 tcaaaacagc gtacaatcag tacgttacca aactattttc tttattggca cagttactgc
     8581 ataatagtaa ccagtggctc attcaccgac ttatgtcagc cccttcggga cgtgctacat
     8641 aaaatacgaa tgacgcacaa caaggtgcct gccgtccaac ttctgatatc agcgtagcta
     8701 tatcaaccat cgggcgaaac gtcgagttag gcaccgcctt attccataac aaagccgggt
     8761 aattcccggc tttgttgtat ctgaacttcc cctcggttag catcaggcta ttcgcgtctg
     8821 acgagagtaa caccttgaaa cgctggcccg tttttccccg ctcattacga caactggtaa
     8881 tgctggcatt tttgctgatt ctgctgcccc tgttggtgct ggcatggcaa gcctggcaaa
     8941 gcctgaatgc gcttagcgat caggcggcgc tggttaaccg cactacgctt attgatgccc
     9001 ggcgtagtga agcgatgacc aacgcggcgc tggagatgga gcgtagctac cgtcagtatt
     9061 gcgtgctgga cgaccctacg cttgcgaagg tttatcaaag ccagcgcaag cgttacagcg
     9121 aaatgctcga tgcccacgca ggcgtactgc cagacgataa actttaccag gcattgcgtc
     9181 aggatttgaa caatctggcc caacttcagt gtaacaacag tggtcccgat gctgccgccg
     9241 ccgcgcgtct tgaagccttt gccagtgcca ataccgaaat ggtgcaggcc acgcgcacag
     9301 tggtgttctc tcgtgggcag caacttcagc gtgaaatcgc cgaacgtggg caatattttg
     9361 gttggcaatc gctggtgcta tttctggtga gtctggtgat ggtgctactt ttcacgcgga
     9421 tgattatcgg gccggtgaaa aatatcgagc gcatgatcaa ccgtctgggg gaagggcgtt
     9481 ctctgggcaa tagcgtttcg ttcagtggac cgagcgagtt acgctcggtt gggcaacgta
     9541 ttctttggtt aagtgagcgc ctgtcatggc tggaatccca acgccatcaa tttttaagac
     9601 atttatctca tgaattaaaa acgccactgg cgagtatgcg cgagggcact gaattactgg
     9661 ctgaccaggt tgtcgggccg cttacgccag agcaaaaaga ggtggtgagc attcttgata
     9721 gcagcagccg caatttgcaa aaactgatcg aacaactgct tgattacaac cgtaaacagg
     9781 cggacagtgc ggtggaactg gagaatgttg aattagcacc gctggtggag acagtggttt
     9841 ctgctcatag cctgcccgca cgggctaaaa tgatgcatac cgacgtcgat ctcaaagcaa
     9901 cagcttgcct ggcggagcca atgctgctga tgagcgtact ggataatctt tactccaatg
     9961 cggtgcacta cggggctgaa tccggtaaca tttgccttcg cagcagttca catggtgcgc
    10021 gggtttatat tgatgtcatc aatacaggca cgcccattcc gcaagaggaa cgcgccatga
    10081 tcttcgaacc cttttttcag ggaagccacc agcgaaaagg ggcggtgaag ggcagcggtc
    10141 tgggattaag cattgccagg gattgtattc gccgtatgca aggggaactg tatctggtcg
    10201 acgagagcgg gcaagacgtt tgtttccgca ttgatttacc gtcgtcgaaa aacacgaaat
    10261 aatgctaaca gcccatccca tcaggctatt ttatttgtca gttcggcccc gggcagtgct
    10321 cgaaatcttc acgtacgaca tgtacgctct ggtttctccg cgctgtccat gtccaaactg
    10381 cctgcaacaa ttacgcccac tgggataggc tttaagtctg gtgaatatgc gacacatttt
    10441 tcaacgatta ttgccccgac ggctgtggct ggcagggctg ccatgtctgg cattgctggg
    10501 ttgcgtgcag agtcacaata agccagccat tgatacgcca gcagaagaaa aaattccggt
    10561 ttatcaactg gctgactatc tttctactga atgtagcgac atctgggcgc tgcaaggtaa
    10621 atcaaccgaa accaatccgc tttactggct gcgggcgatg gattgtgctg atcgtttaat
    10681 gcctgcgcag tcgcgtcagc aagcccgcca atatgacgac ggcaactggc aaaatacctt
    10741 taaacaaggg attttgctcg ccgacgccaa aattacgccg tatgagcgcc gccagctggt
    10801 cgcgcgcatt gatgcgctaa gtaccgagat cccggcacag gttcgccctc tgtatcaact
    10861 ctggcgcgac ggtcaggcgc tgcaactgca actggcggaa gagcgccaac gctacagtaa
    10921 actccagcag tccagcgaca gcgagctgga tactttgcgc cagcaacatc acgttctaca
    10981 acagcaactg gaactcacta cccgcaagct ggaaaacctg accgatattg aacgccagct
    11041 ctcgacccgc aaaccggctg gaaatttctc gccggatacg ccgcacgaga gtgaaaagcc
    11101 cgcgccatcc actgatgagg tcactcctga tgagccataa acctgcgcat ttattactgg
    11161 tcgatgacga tccgggattg ctgaaactgc ttggcctgcg cctgaccagc gaaggctaca
    11221 gcgtggtcac ggcggaaagt ggtgctgaag gattacgggt actgaatcgc gaaaaagtag
    11281 atttagttat cagcgacctg cggatggatg aaatggatgg tatgcagctg tttgctgaaa
    11341 tccagaaagt gcagccggga atgccggtaa ttattcttac cgcacatggt tctattcccg
    11401 atgccgttgc tgcaacacag cagggagtgt tcagtttcct caccaagcct gtcgataaag
    11461 acgcgctata tcaggcaatt gacgatgcgc tggaacaatc cgcgccagcc accgatgaac
    11521 gctggcgcga ggcgattgtc acccgcagcc cgctgatgct gcgtttgctg gaacaggcgc
    11581 ggctggtggc gcaatcagac gttagcgttt tgattaacgg tcagagcggc accgggaaag
    11641 agattttcgc ccaggctatc cacaacgcca gcccgcgcaa tagcaaacca tttattgcta
    11701 ttaactgtgg tgcattgccc gagcaattgc tggagtcgga gctgtttggt catgcgcgtg
    11761 gcgcgtttac tggcgctgtc agcaatcgcg aaggtttatt ccaggcggcg gaaggcggta
    11821 cgctatttct cgatgagatt ggcgatatgc ctgcaccgtt gcaggtcaaa ctgctgcgtg
    11881 tgttgcagga gcgtaaagtg cgcccgctgg gcagtaaccg cgatattgat atcgacgtgc
    11941 ggattatttc cgccactcac cgcgacctgc caaaagcgat ggcgcgcggg gaattccgtg
    12001 aagatctcta ttaccgcctc aacgttgtca gcctgaaaat tccggctctg gcagagcgca
    12061 cagaagacat tccgctactg gcaaatcacc tgttgcgcca ggcggcagag cgacataaac
    12121 cgtttgtccg cgcgttctcc accgatgcga tgaaacgcct gatgaccgct agctggccgg
    12181 gtaatgtacg ccagttggtc aacgtgattg aacagtgcgt ggcgctgacc tcatctccgg
    12241 tgattagcga tgcattagta gagcaggcgc tggagggcga aaacacggcg ctgccaacct
    12301 ttgttgaggc acgtaatcag tttgaactca actatttgcg taagctgctg caaatcacca
    12361 aaggcaacgt cacccacgcg gcgagaatgg cggggcgcaa ccggacagaa ttttataaac
    12421 tgctttcccg acacgagctg gatgcaaacg atttcaagga atgaattggc gttatgtgtt
    12481 acgtttagca gatcaaaaga caggcgacct tttcaaggaa tagcatgaaa aagattgatg
    12541 cgattattaa acccttcaag ctggacgatg ttcgcgaagc actggctgaa gtcggcatta
    12601 ccggcatgac ggtcactgag gtgaaaggtt ttggccgcca gaaaggccat accgagctgt
    12661 accgcggcgc ggagtatatg gtggattttc tgccgaaagt gaaaattgag attgtcgtac
    12721 cggacgacat tgtcgatacc tgtgtcgata ccattattcg cacggcgcaa accggcaaaa
    12781 tcggtgacgg taaaatcttc gtctttgacg tggcacgggt cattcgtatc cgtaccggtg
    12841 aagaagacga cgcggcaatt taaaccgcgt caagggttgc cggatgtttc catccggcaa
    12901 catcaaatca cagcacctta tgcgggccaa agcattcgta atgaatattt tcctgcttca
    12961 cgcccagatc cactaactgt ttcgcggcaa actgcatgaa gccaaccggg ccgcagagat
    13021 agaactgcat tgtcggatcg ctgaacgcac cttccagttt gctcaaatcc atcagacctt
    13081 cgctatcaaa ctgaccttta gcgcgatcgg cttcgctcgg ctgacgatac caggtgtgcg
    13141 cggtaaagcg cggcagtgac agccccagtt ccttaacttc atcagcaaag gcgtgaacat
    13201 cgccattttc tgccgcatgg aaccagttca cttgtgctgt gtggcctgct tttgccagtg
    13261 tgtcgagcat tgccagcatt ggcgtttgac cgacaccggc agagattaac gtcactggtg
    13321 tgtcatctgc gacagccata aaaaaatcac ctgctggagc gaccagtttc acgacatcgc
    13381 caacattggc gtgattgtgc agccagttgg atacctgccc accctcttcg cgtttcaccg
    13441 caatacgata gcctttgcca tccggtttgc gagtcaaaga gtactgacga atttcctgat
    13501 gcgggaaacc ttccggcttc agccagacgc cgagatattg ccccggacgg tattctgcca
    13561 ctgcgccacc gtcgaccggc tccagttcga agctggtgat aagcgcgctg cgcggtgttt
    13621 tagccacaat gcggaaatcg cgagtacctt cccagccacc ggctttgctg gcgttttcgt
    13681 tatagatttc cgcctcgcga ttgataaata cattagccag tacgccatag gctttacccc
    13741 acgcgtccag cacttcctgg cccgggctga acatttcgtc cagcgtcgcc aacaggtgtt
    13801 caccgacaat gttgtactgt tccggtttga tctggaagct ggtgtgcttc tgcgcgattt
    13861 tttctaccgc tggcagcagc gcaggcaggt tttcaatatt actggcgtag gcggcaatag
    13921 cgttaaacag ggcttcacgt tgatcgccat tgcgctggtt actcatgtta aaaatttctt
    13981 tgagttctgg gttatgagta aacatacggt cgtagaaatg ggcggttaac tttggtcccg
    14041 tttccaccag taaagggatg gtggctttta ctgtagcgat ggtttgagcg tcaagcatat
    14101 ggtcttcctt ttttttgcat cttaattgat gtatctcaaa tgcatcttat aaaaaatagc
    14161 cctgcaatgt aaatggttct ttggtgtttt tcagaaagaa tgtgatgaag tgaaaaattt
    14221 gcatcacaaa cctgaaaaga aatccgtttc cggttgcaag ctctttattc tccaaagcct
    14281 tgcgtagcct gaaggtaatc gtttgcgtaa attcctttgt caagacctgt tatcacacaa
    14341 tgattcggtt atactgttcg ccgttgtcca acaggaccgc ctataaaggc caaaaatttt
    14401 attgttagct gagtcaggag atgcggatgt taaagcgtga aatgaacatt gccgattatg
    14461 atgccgaact gtggcaggct atggagcagg aaaaagtacg tcaggaagag cacatcgaac
    14521 tgatcgcctc cgaaaactac accagcccgc gcgtaatgca ggcgcagggt tctcagctga
    14581 ccaacaaata tgctgaaggt tatccgggca aacgctacta cggcggttgc gagtatgttg
    14641 atatcgttga acaactggcg atcgatcgtg cgaaagaact gttcggtgct gactacgcta
    14701 acgtccagcc gcactccggc tcccaggcta actttgcggt gtacaccgcg ctgctggaac
    14761 caggtgatac cgttctgggt atgaacctgg cgcatggcgg tcacctgact cacggttctc
    14821 cggttaactt ctccggtaaa ctgtacaaca tcgttcctta cggtatcgat gctagcggtc
    14881 atatcgacta cgccgatctg gaaaaacaag ccaaagaaca caaaccgaaa atgattatcg
    14941 gtggcttctc tgcatattcc ggcgtggtgg actgggcgaa aatgcgtgaa atcgctgaca
    15001 gcatcggtgc ttacctgttc gttgatatgg cgcacgttgc gggcctggtt gcagctggcg
    15061 tctacccgaa cccggttcct catgctcacg ttgttactac caccactcac aaaaccctgg
    15121 cgggtccgcg cggcggcctg atcctggcga aaggtggtag cgaagagctg tacaaaaaac
    15181 tgaactctgc ggttttccct ggtggtcagg gtggtccgtt gatgcacgta atcgccggta
    15241 aagcggttgc tctgaaagaa gcgatggagc ctgagttcaa aacttaccag cagcaggtcg
    15301 ctaaaaacgc taaagcgatg gtagaagtgt tcctggaacg cggctacaaa gtggtttccg
    15361 gcggcactga taaccatctg ttcctggttg atctggttga taaaaacctg accggtaaag
    15421 aagctgacgc cgctctgggc cgtgctaaca tcaccgtcaa caaaaacagc gtaccgaacg
    15481 atccgaagag cccgtttgtg acctccggta ttcgcgtggg tactccggcg attacgcgtc
    15541 gcggcttcaa agaagcagaa gcgaaagaac tggctggctg gatgtgtgac gtgctggaca
    15601 gcatcaatga tgaagccgtt atcgagcgca tcaaaggtaa agttctcgac atctgtgcac
    15661 gttacccggt ttacgcataa gcgaaacggt taatctgttg aaaaaggctt ccttcgggaa
    15721 gcctttctac gttatcgcgc catcaaatct gtcgtaactg cgcctcaaca tacaaatagc
    15781 caatccccag cacctgttgt gcgcggctta attgcccaaa gccaattttc gtcgctttca
    15841 cgctcggtcc ttcgtcgcgg tcgaacgggt taaatcccgt ctggcgaata atggtattaa
    15901 gccagtgctc accaaaggca caactgcgac cgtacaacca aatctgatta atattgagga
    15961 tgttgaggaa gttatacaga ctcaaaccaa tggcattggc agagcgatca acccagctgg
    16021 tgatccacgg ttctccactc tgccaggcag cgattaactg cgctgttgtc agtttttcag
    16081 gatcaagctg agtattaacc ggttgtgatt ttagccatac ccgcgcctgt ttttttaatg
    16141 cacttaacga ggcgacggtt tccaggcagc cgtaacgtcc acagtcgcag acgacgccat
    16201 ccggattaac gatggtgtga ccaatctgtc cgctaccata caaacagccg cgataaattt
    16261 gctcgttaat cacgaacgac gagccaatgc cgtaatcaac gttgatcacg cagaaatccc
    16321 gttcctgcga attattttgc catttctccg ccagcgccag catcacgcag tcattatcga
    16381 ccatcacccg aatgccgagc ttctcttcca gcagatactt tacctcaacc ggcgttgtcc
    16441 acggcgcttg cggcatggtt tgcgacacgc cggtcaccgg atcaacctga ccgtggattg
    16501 ccagcgccag gttgatggta cggtccggcc acaatttacg gtggcgatgc cagcattttt
    16561 cgatttcgga cagcagcgcc tgcggcgtcg gtgcatcaat ctgtaaatac tcaaattcac
    16621 ctttcggact taaacaagca ttagcaacct gacactcaat actggtgggc gtcacgttca
    16681 ggcacaacgt ccagtcacct tccggcgcaa tcagccatgt accgctacta tgcccgcgcg
    16741 tctggctttc atcgtcgata ttcaccaccc gtttttcgct ttccagttcc tgcaaaatat
    16801 tactgaccgc cggaatcgaa atttgcgcca gccgggccag cgttgattta ttggcgcgct
    16861 tttgccggta cagcagttcc agaatcacgc atttgttatg gtggcgaatc tgttgattat
    16921 taatgcaggc tctcatcttc actaaacctt gtttagttaa ttgcgtgatt attccgcgaa
    16981 aacattgcca gattgttcaa tacactgcca caaatctttt aattcagtat gtcttgttaa
    17041 tattgagggc accatgactc cagtaaaagt gtggcaagag cgcgttgaga tcccgaccta
    17101 tgaaaccggg ccgcaggata tacatcccat gttcctggaa aatcgcgttt atcagggatc
    17161 gtccggcgcg gtttatccct acggcgtgac cgatacgctg agcgagcaga aaaccctgaa
    17221 atcctggcag gcggtgtggc tggaaaacga ctacatcaaa gtgatgatcc tgccggagct
    17281 gggcggtcgg gtgcatcgcg cgtgggataa agtgaaacag cgcgattttg tctatcataa
    17341 tgaagtcatt aaaccggcgc tggtggggtt gctggggccg tggatctccg gcgggattga
    17401 gtttaactgg ccgcaacacc atcgcccgac cacctttatg cccgtcgatt tcaccctcga
    17461 agcccatgaa gacggcgcac aaaccgtgtg ggtcggcgaa acggagccga tgcacggttt
    17521 acaggtgatg acaggtttca ccctgcgccc tgaccgggcg gcactggaaa ttgccagccg
    17581 cgtctataac ggcaacgcca cgccgcgtca tttcttgtgg tgggccaacc cggcagtgaa
    17641 agggggtgaa gggcatcaga gcgtcttccc gccggatgta acggcggtgt ttgatcacgg
    17701 caaacgggcc gtctccgctt tccccatcgc caccggcact tactacaaag tggactactc
    17761 cgccggagtg gacatttctc gctataaaaa tgtgcccgtt ccaacctcat atatggctga
    17821 aaaatcacag tacgattttg ttggcgcgtg gtgtcacgat gaagatggcg gtttgctgca
    17881 cgttgccaac caccatattg cgccaggtaa aaaacagtgg agctggggac acagtgaatt
    17941 tggccaggcg tgggataaga gtctgaccga caataacggc ccgtatatcg aactgatgac
    18001 tggtattttt gccgataacc agcccgattt tacctggctt gatgcttacg aagagaagcg
    18061 ttttgagcag tatttcctgc cttaccattc tttgggcatg gtgcaaaacg cctcccgcga
    18121 tgcggtgatt aaactccagc gtagcgagcg ggggattgag tgggggctgt atgccatctc
    18181 tccgttgaaa ggataccgtc tggcgatccg cgaaatcggc aaatgcaacg cgttgctcga
    18241 tgatgccgtg gccctgacac cagcgaccgc catccagggc gtgttgcacg gtatcaatcc
    18301 tgaaaggctg accattgaac tctccgatgc cgacggcaat atcacactga gttatcagga
    18361 acatcagccg caagagttgc cgttgccgga cgtcgccaaa gcgccactgg cagcacaaga
    18421 cattaccagt acagatgaag cctggtttat cggtcagcat ctggagcaat atcatcacgc
    18481 cagccgttca ccgttcgatt actacctgcg cggcgtggcg ctggatccgc tggattatcg
    18541 ctgtaacctg gcgctggcga tgctggaata taaccgagca gatttcccgc aagcggtggc
    18601 gtatgccact caggcgctga aacgcgcaca tgcgctaaac aaaaatccgc agtgcggaca
    18661 ggcgagtttg attcgcgcca gtgcttacga acgtcagggg caatatcaac aagccgaaga
    18721 ggatttctgg cgggcggtct ggagcggcaa cagtaaagca ggtggctatt atggtctggc
    18781 acggctggcg gcgcgtaatg gtcacttcga cgctggtctg gatttttgcc aacaaagtct
    18841 tcgcgcctgc ccaaccaatc aggaagtgct ttgcctgcat aatctgctgc tggtgttaag
    18901 tggtcgtcag gacaacgcgc gtttgcagcg cgagaaactg ctgcgtgatt atccgctgaa
    18961 cgccactctg tggtggctga actggttcga tggtcgtagc gaatcagccc tcgcgcagtg
    19021 gcgcggtctg tgtcagggac gcgacgttaa cgccctgatg accgccgggc aactgattaa
    19081 ctggggaatg cccaccctgg cggcagagat gctgaacgca ctggactgcc agcgcacgct
    19141 gccgctttac ttgcaagcca gcttgctgcc gaaagctgaa cgcggcgaac tggtcacaaa
    19201 agccattgat gtcttcccac agtttgtccg tttcccgaat acgctggaag aagtggcggc
    19261 gctggagagt attgaagagt gctggtttgc ccgtcattta ctggcctgct tctactacaa
    19321 caagtgcagc tacaacaaag ccattacttt atggcaacgt tgcgtagaga tgtcgccgga
    19381 gtttgccgac ggctggcgcg ggttagcgat ccatgcgtgg aataagcaac acgattatga
    19441 gctggccgcg cgttatcttg ataatgctta tcagcttgcg ccgcaggatg cacgtctgct
    19501 tttcgaacgg gatttgctgg ataagttaag cggtgtcaca ccggagaaac gactggcgcg
    19561 tctggaaaat aatctggaaa ttgcgctgaa acgcgacgac atgaccgcag aactgctcaa
    19621 tttgtggcat ctcacgggtc aggcagacaa agcggcggac attctcgcca cgcgcaaatt
    19681 ccacccgtgg gaaggcgggg aagggaagat caccagtcag tttatcctca accagttatt
    19741 acgcgcctgg cagcatcttg atgccagaga gccgcagcag gccagcgaac tgcttcatgc
    19801 cgcgttgcat tatccggaga atttaagcga aggccgttta ccggggcaaa ctgacaacga
    19861 catctggttc tggcaggcga tatgcgccaa cgcgcagggc gatgaaactg aagcgacgcg
    19921 ttgtttacgt ctggcggcga ctggcgatcg caccattaac attcacagtt attacaacga
    19981 tcagccggtt gattatctct tctggcaagg aatggcgctg cgactgctgg gcgaacaaca
    20041 caccgcacag caactgttta gtgaaatgaa acagtgggcg caagagatgg cgaaaaccag
    20101 tatcgaggcg gatttctttg ctgtttcaca acctgacctg ttgtcgctgt atggcgattt
    20161 acaacagcag cataaagaaa aatgcctgat ggtggcgatg ctggcgtccg cgggactcgg
    20221 ggaggttgcg cactatgaat ctgctcgcgc tgaattgatg gcgattaatc cagcctggcc
    20281 gaaagcggca ttattcacca ccgtgatgcc ttttattttt agctacgttc actaaacctt
    20341 attaactgta atatttgagc ggcacgcgct gccgctcacg ctcaaataat taaaataagc
    20401 cgttgtaaat aaaaacccta caggaggcac tatgcctaaa aaaatgacaa gaacccgtaa
    20461 tttattatta atggctacgc tattgggtag cgcactattt gccagggctg cggataaaga
    20521 aatgactatt ggcgcaatat accttgatac ccagggatat tacgccggag tgcgtcaggg
    20581 cgttcaggat gcggcaaaag attcttcagt acaggtacag ttaattgaaa ctaacgctca
    20641 gggcgatatt tcgaaagaaa gtacctttgt tgataccctc gtggcgcgta atgtcgatgc
    20701 cattatttta tcggcagtgt ctgaaaatgg cagtagtcgt accgttcgtc gcgccagtga
    20761 agcgggtatt ccggtgattt gctacaacac ctgtattaat caaaaaggtg tcgataaata
    20821 tgtctcggcg tatctggtcg gcgatccgct ggaatttggt aaaaaactgg gtaacgctgc
    20881 cgccgattat tttattgcca ataaaattga ccagccgaaa attgccgtca tcaattgcga
    20941 agcctttgaa gtttgtgtgc agcgacgtaa aggatttgaa gaggtattaa aatcccgcgt
    21001 tcccggcgcg caaatagtcg ctaatcagga agggactgtt ttagataaag cgatttccgt
    21061 tggtgaaaaa ctaattatct ccacgccgga tctcaacgcc attatggggg aatcgggcgg
    21121 tgcgacactc ggcgcggtaa aagcggtacg taatcaaaat caggccggaa aaattgctgt
    21181 tttcggttcg gatatgacca ctgaaattgc tcaggagctg gaaaacaatc aggtgctgaa
    21241 agcggtggtg gatatttccg gtaagaaaat gggcaatgct gttttcgcgc aaactttgaa
    21301 ggttatcaat aaacaagccg acggtgaaaa agtgattcag gtgccaatcg atctctatac
    21361 caaaacggaa gacggtaaac agtggctggc aacgcacgtt gatggtctgc cctaatcgct
    21421 gattcgcaga ggtccctatg ttcacagcaa cagaggcagt cccggtagca aaagtggtgg
    21481 caggaaataa gcgttatccc ggcgtcgttg cgctggataa cgttaacttc acgctcaata
    21541 aaggcgaagt tcgtgcgctg ttaggcaaaa acggtgcggg aaaatcgacc ctcattcgaa
    21601 tgcttaccgg tagcgaacgt ccggatagcg gtgatatctg gattggcgag acgcgactgg
    21661 aaggtgacga agctacgctg actcgccgtg ccgctgaact gggggtacgc gcggtttatc
    21721 aggagttaag tctggtggaa gggctgacag tggcggaaaa cctctgcctc ggtcagtggc
    21781 cccgccgcaa cggcatgatt gattacctgc aaatggcgca ggacgcccaa cgttgcttgc
    21841 aggcgttggg cgttgacgtt agtcctgaac aacttgtttc aacactaagc ccggcgcaaa
    21901 agcagctggt ggaaattgcg cgggtgatga agggcgagcc gcgcgtggtc attcttgatg
    21961 aacccaccag ctcgcttgcc agtgcggagg ttgaactggt gatcagcgcg gtgaaaaaga
    22021 tgtcggcact gggcgtggcg gtgatttatg tcagccaccg gatggaagaa attcgccgca
    22081 ttgcctcctg tgccaccgtt atgcgcgatg gtcaggtggc gggcgatgtg atgctcgaaa
    22141 acacctccac gcatcatatt gtgtcgctaa tgctcgggcg cgatcacgtt gatattgcgc
    22201 cggtttcccc tcaggaaatt gtcgatcagg ccgtgctgga agtccgtgcg ttacgccata
    22261 agcccaagct ggaggatatc agctttacgc tccgtcgtgg cgaagtactc ggcattgctg
    22321 gcctgctggg ggcagggcgc agtgaattgt tgaaagccat agttgggctg gagacgtatg
    22381 aacagggcga aattgttatc aacggcgaga aaatcactcg ccccgattac ggcgacatgc
    22441 tgaaacgcgg cattggctat acgccagaaa accgcaaaga tgcggggatc attccctggc
    22501 tgggcgttga cgaaaataca gtgctgacca atcggcaaaa aatcagcgcc aacggtgtgc
    22561 tgcaatggtc caccatccgc cgcctgaccg aagaggtgat gcagcggatg acggtcaagg
    22621 ccgccagtag cgaaacgccc atcggcacgc tttctggcgg caatcagcaa aaagtggtga
    22681 tcggtcgttg ggtctatgcc gccagccaga ttttgttgct cgacgagcca acgcgtggcg
    22741 tcgatatcga agccaaacag cagatttacc gtattgtccg cgagctggct gccgaaggaa
    22801 aaagcgtggt gtttatctcc agtgaagtgg aggagttgcc gctggtgtgc gaccgcattc
    22861 tgttgttaca gcacggtacg ttctcgcagg agtttcacgc tccggtcaat gtggatgagc
    22921 tgatgtccgc cattctgtct gtgcactgat gaattttacc gaggaacctg aagatgtctg
    22981 cttcgtcatt actattgccg cagggcaaga gcgtctcgct caaacaattt gtcagtcgcc
    23041 atattaatga gatcggtttg ctggtggtga tcgccattct ttatctggtc ttctctctga
    23101 acgcgcctgg ctttatctca ttgaataacc agatgaacgt gctgcgcgat gccgccacca
    23161 tcgggatcgc cgcatgggcg atgacgctga ttattatctc cggtgaaatt gatgtcagcg
    23221 ttggtccgat ggtggctttt gtctcggtgt gcctggcatt tttgctgcaa tttgaagttc
    23281 cgctggcggt ggcgtgtctg ctggtgttgc tgttaggcgc gctgatggga acgctcgccg
    23341 gggtgctgcg cggcgtgttt aacgtgccaa gttttgttgc cacgctgggt ttatggagcg
    23401 ccctgcgcgg aatggggctg tttatgacca acgccttgcc agtgccgatt gatgaaaacg
    23461 aagtgctgga ctggctgggg ggacaatttc tcggtgtgcc ggtatccgcg ctgatcatga
    23521 tggtattgtt tgcgctgttt gtgttcatca gccgcaaaac cgccttcggg cgctcggttt
    23581 ttgccgttgg cggtaatgcc actgcagcac agttgtgcgg tattaacgtt cgtcgggtac
    23641 gcattcttat ctttaccctt tcgggattat tagcggcggt gaccggcatt ttgttggcgg
    23701 cgcgcctcgg ttccggtaac gcaggtgccg caaacggtct ggagtttgac gtcatcgccg
    23761 cggtcgtggt tggcggtacg gcgctttccg gtggtcgcgg ctcgttgttc ggtacgctgc
    23821 ttggcgtact ggtgattacg ctaatcggta acggtctggt gctgctcggg attaactcct
    23881 ttttccagca ggtggtgcgc ggcgtcatca tcgtggtggc ggtgctggcg aatatcttgc
    23941 tgactcagcg tagcagtaaa gcgaaacgct aaaccttaac gcccctggcg cgggtgcggc
    24001 agggatgaaa attaatcgta ttgttaatga gggatttatg aaaacgatgc tggcagctta
    24061 tttaccagga aattcgaccg tcgatctgcg ggaagttgcg gtgccgacgc cggggattaa
    24121 ccaggtactg atcaaaatga aatcctccgg gatttgcgga agcgatgtcc actatatcta
    24181 tcatcaacac cgtgccacgg cagcggcacc cgataaaccg ttataccagg gatttatcaa
    24241 cggtcatgaa ccgtgcgggc agattgtggc gatggggcaa ggctgccgcc attttaaaga
    24301 gggcgaccgt gtgctggtgt atcacatttc tggctgtggt ttttgcccga actgccgtcg
    24361 cggctttcct atctcttgta ctggcgaagg aaaagcggct tacggctggc agcgtgacgg
    24421 cggtcatgcc gaatatctgc tggcggaaga aaaagatctg atcctcctgc cggatgcact
    24481 gagttacgaa gatggtgcgt ttatcagttg cggcgttggt acagcgtatg aagggatttt
    24541 gcgcggcgaa gtttccggca gtgataacgt actggtggtc ggtctggggc cggtcggcat
    24601 gatggcgatg atgctggcga aaggtcgcgg tgcaaaaagg atcatcggcg ttgatatgct
    24661 gccggaacgt ctggcgatgg cgaaacagtt aggagtgatg gatcacggct atttagcgac
    24721 caccgaaggt ctgccgcaga taatcgccga actcactcac ggcggcgcgg atgttgcgct
    24781 tgattgttcc ggtaatgccg caggtcgctt actggcactg caatccaccg ctgactgggg
    24841 acgcgtggtt tacattggtg aaaccggaaa agtcgagttc gaggttagcg ccgacctgat
    24901 gcaccatcaa cggcggatta tcggctcctg ggtgaccagt ctgttccata tggaaaaatg
    24961 cgcccacgat ttaacggact ggaaactgtg gccgcgtaac gccattaccc accgcttctc
    25021 gctggaacag gcgggagatg cttatgcact gatggcaagc ggcaaatgcg ggaaagttgt
    25081 gattaacttc ccggattaag gagttagcgg atgaccatct ataccttatc ccacgggtcg
    25141 ctaaagctgg gcgtttctga ccagggcggc gtaatcgaag gcttctggcg cgatacgacg
    25201 ccgctcttac gtcctggtaa aaaaaacggt gtggcaaccg atgcctcttg ttttccgctg
    25261 gtgccatttg caaaccgggt gagcggcaac cgttttgtct ggcaggggcg tgagtatcaa
    25321 ctgcaaccga atgttgagtg ggatgcgcac tatctgcacg gcgacggctg gctggggcag
    25381 tggcaatgtg tctcgcgcaa tgatgatatc ttgtgtctgg tgtatgaaca tcgcagcggt
    25441 gtttatcact atcgggtaag ccagacgttt catttaacgg cggatacgct gacggtgaca
    25501 ctctctgtca ccaatcaagg ggcagagacg ctgccatttg gtaccggctg gcatccttat
    25561 tttccgttgt cgccgcaaac gcggattcag gcacaggcga gcggttactg gctggagcgg
    25621 gagcagtggc tggcgggtga gttttgcgag cagctaccgc aagaattgga ttttaaccag
    25681 ctcgcgccgt tgccgcgcca gtgggtgaac aatggttttg ccggatggaa tggtcaggcc
    25741 cgcatcgagc agccgcagga gggttatgcc atcatcatag aaacgacgcc acctgcaccg
    25801 tgttatttca tctttgtttc cgacccggcg tttgataaag gatatgcgtt tgatttcttc
    25861 tgtctggaac cgatgagcca tgcgccagat gaccatcatc gcccggaagg tggtgacctc
    25921 attgcgcttg caccagggga atcaacaact tcagagatgt cgttgcgggt tgcgttactg
    25981 taatttataa aatgccggat aaggcgttca caccgcatcc ggcactcggc gcacgatgcc
    26041 tgatgcgacg cttgccgcgt cttatcaggc ctacaattgg tgttggaacg gtaggcagga
    26101 taaggcgttc acgccgcatc cggcatcttt cctaacgccg atcgagagaa atcgcccccg
    26161 gcccggtaat tgccaacagc aggaatgcgc cagcgatact gacattcttc cagaaattaa
    26221 tcatatttgg cccaaccgca tcgccggtca tatcccagta atggtgacca atcaccgccg
    26281 tacccagcgt gtagaaaata aacagcaccg ccagcggacg ggtgaaaaag ccaagcacga
    26341 ttaatatcgc ggcgggcact tccataacta ccgcaataat cgctgccagc atcggcatcg
    26401 gcgcgcccag cgaggccata tattggaccg taccgtcaaa gcccatcatt ttgggaaaac
    26461 caaaaataat gaaaattaag accacggcga tacgggcaat taataacaaa acggggcgtg
    26521 cagcaccaaa atcaaaataa cgtagtgagt tcataaccaa tccctttgca agataatgtg
    26581 atttaaacgt aatacacact cttgcacctc gccacgcccc tggcattgag atattcgcta
    26641 attatcctgg ttacagtgat ttaagcgcga tgtcctcatc tatcagcagt ttcgcatcaa
    26701 acgttttgcc gctctggatc catttgcgga ttaagcgaat ctcacgcccc tgattcagcg
    26761 ttaccgcacc gataagcacg ccgttttgca gattaaacca aatcgccttc tgagtttccg
    26821 ggttgccacg acaaatccag tcatcgccat gcatatcgcc aataaactgt aagttatcac
    26881 tgtactgatc gctccagaac cacggcggcg gcagtcgcgg aagcggtagc cccaacattg
    26941 cggcagcggc aatttgcgcc tggttattgg cgttttccca gctttcgcag cggtgtagtg
    27001 caccattatc aatacgggtg attgccacat cgccaccggc aaagatcgcg ggatcgcagg
    27061 tgcggcaagc ttcatcaatg acaataccat tggcagtatc aaggttggcc tcgcgagcca
    27121 gttggtcgtt ggcgctgata ccaataccgt aaatcaccac atcggcccga agcgtttcgc
    27181 cactttgcag cgtcagttct actttttcac catcgaccac atgttcaatg gcattattga
    27241 gcagaatgcg cacaccagcc tgctgatggc gcggtaaaag atagcgttgc acgggcggtg
    27301 gtgcattacg gcccatgacg cttgccgcca gttcaatcac tgtcacctta catccacgct
    27361 gcgtggcgct ggcagccagt tccagaccaa tagttccggc accgacaatc acgactgacc
    27421 gttcgggctg cagaacttct cgcagtctgg cggcatcacc ggcatggcgc agggtaaagc
    27481 agcgttctcc cagtgcatca agcaacggca gcggtcgagc tgccgcgccg gttgctataa
    27541 aaagctgatc ccagtgccag ctttcgccgt tagctaacac taactcttgt gtgtcgcggc
    27601 ccaatgtttt gatggttaca ccggaatgca gatgaacatt gttttcctgc caccagtgag
    27661 cgggtaacac agactgcaac tgtggggaat cttccagcaa catggatttc gagagcgggg
    27721 ggcgttcata aggaagatgt tgctcatcgg aaaacagatg cagctcaccg ctgaaccctt
    27781 gctggcgtag caaggccgca gccattgccg ccgcttgccc gccaccgaca ataatgatcg
    27841 ttttttcttt catcgtgctt ccttaaagat ccagcccagc cgctacgtgg cgaatgccgc
    27901 gaatcgctaa acccgcatca gcgttgatca tcacaccgct taatgcgcga ttattgcgcc
    27961 gcgatgccaa catcacatac ggacccgtaa aatccgccgg ttgcgggaaa aattgcagcg
    28021 gtaaaatggc ggcaattttc tccggcgtca gagactgcat tatcgaggtt tcactttgcc
    28081 cgagcgcctg tgggccgcgc aggtcgctgg ccataccaca cgggccgacg ccattcaccc
    28141 gcactttcgg tgccagttca taagccagtt ggcgaataag tccggttgcg gcatgtttac
    28201 tggcggtgta cagcgggcca ccgccgccgg gataccaggc ggcgtttgac agtgtgaaaa
    28261 tcatgctgcc ttcactggcg attaacgccg gagcgcaggc ttttgcgccc agcaggtaac
    28321 cgagtacgtt gacattaaac agctcgtgga agccggtttc gagcgtctct gcgggagtgt
    28381 taaccagtga ggcattgtga tcccagatgc ccgcattgcc gataaaacaa tccagcttgc
    28441 cagaacgagt caggatctga ttaaccgcgc gttgataatc ggcatagcag gtcacgttac
    28501 cttccaccgc cagaatatgt tcgccaaatc gctgacgcag actggcgact tttgccgccg
    28561 acagttccag cgtggcaacc tgcgcgcctt cttcgataaa tcgctcggcc agcgccaggc
    28621 ctaatcccga tccgccgccg gtaataaaga tggactcgtt atgcagatcg ctcatggctg
    28681 cgcctccggt aagtcgatga agatgtcacc accttcaacg tgtactggat aagtggttag
    28741 cggatcggtg gcgggcaggc ataacgcttt ccccgttttc aggcaaaaac tggcggcgtg
    28801 cagcgggcac tccaccgtgg cgtcatcttc cagataccct tctgacatcg acgcattacc
    28861 atggctgcaa cgatcgttaa tggcataaaa ctcgccgcct acgttgaaca gggcgatgac
    28921 gggcgaggta tcgatccgga gagcctcacc ctccggcaca tccgcaacgg gacacgcata
    28981 aattcgattc atcagaacag tacactcagg ttatgggagg agatgaccgc ttgatccagg
    29041 acgatatccc gttccagcaa gcgccagttc ccgtcctcca gacggcgaac tttgtcgaaa
    29101 cgcgttccaa catagaatgt ttcatcgcgc tctttttgtg cccgataaag tagataattc
    29161 accctcacgg caaatacgtt tgggatgtcg gtttcgctta cctggcagtt gctgattaag
    29221 tgacgagtgc gtgacggcgg ttcttctgcc caggccatgc cggtttccag acgggcgatt
    29281 cgccgttcca gctggtcttt ggtgtcatta aaaatccagg ttgtcggcgg ttgaacgcct
    29341 ttgcggcggt cgcgcgtttg tgcgttaacc gtggtgcgca tggtgtaacg aatctcttcg
    29401 tcgagctgtg ccagccagtc acgaaatttc cagtcatcca gcagactggc ttcgtgaaag
    29461 agaaactggc taatgcggtg atgtaactct agtgaaactt gcgcactcat ttcatcacct
    29521 cctgctggta agcggcggtt ttatcgagca cttcctgcca gctttcgcta ctcagaagat
    29581 cggcccagcg ttggtacatt ccacgagcgg ccgtttctga gaagatatag ttagtaatgc
    29641 ctggaatgcc gtcgtcgcga cgcttttcct gaccaagccc catttccaga cacagtttgc
    29701 tgttgcgggc gcggtggcct ttcagcaatt tctggatttc acaccagttc tccgagtcat
    29761 cctgctcgag aaaaccagca ggaccaaaag cacgagtggc gctgttttca aaagcggctt
    29821 taacttcatc ggaggcggct ttgtcagtaa tacagaacgc ccacacttcg acttgatcag
    29881 ggccgcgcgg atgccagacg cggagcgtgg cagtgccgtt gagccatgaa agcgtgggga
    29941 aaatattgtt atgacccgcc aggcgcaggg cgcgaacttc acctaaacgt tgttctgctt
    30001 cggcataggt ttcgcggtaa tagctggaaa ccgcgccatc gacccagaca ttagcatccg
    30061 gtttttcagt aaagaagaaa ccgctaccgt gaccgtcctg accaaattgc agcgcatctt
    30121 tggcggtttc ccacaccggg cgggcggttt gtccatcacc gagacgctta tcgctgccat
    30181 catctttcgc ccctaatacc tgaacggcag aagcatggct gaacagagca tgatattggt
    30241 cactggcgaa ctgctctgcc gggaatttcc agttgcagtt gatcacccac ttttgtacac
    30301 cgccgacaat ttcggtgccg ccttcgcgac gatccagcat gccatccaga taccaggcaa
    30361 tgtcacccag gtaatcacgc aggcccggtg cgctggtatc ccagttgcca aaaatcagcc
    30421 ctttataact ctccacacaa ggaacttcgt ttagtcccca gtgggattta cacaaccctt
    30481 gtgggtaggc gcgaggttcc agcggtacat cgatcaactc gccgttaatg ccataagacc
    30541 agccgtgata cggacaggta aaggcgcgag tgttaccgca atctgcataa ctcacacgca
    30601 tggcccggtg gcggcattgg ttgagaaacg ccttaatgct gccgtctttc tgacgcacta
    30661 cgacaaccgc atcttccccc atgtaggtgt taaagaaatc accatgtttt gggatctggc
    30721 tttcgtgggc gagaaatagc cagcaacgac cgaaaatgcg ctcaagctcc agttggtaaa
    30781 tatccgggtc ggtataaata cgcggagtga cccgaccgtt ttgggtgtca atcagttggt
    30841 aaatgttcaa atctgagggt gtggtcatat tttttaatcc tgaaagataa gatgtattga
    30901 ccttctattt ttgtgccata aggatgttgc tggttggttg ctgatatgtg aaatagttaa
    30961 tttctaccag ctgagacgtt ttttcgataa caaggggaag gtgatggaac tacggcattt
    31021 acgctatttc gtcgcagtgg cgcaggcact gaactttacc cgtgcggcgg aaaaactgca
    31081 tacctcacag ccttcgttaa gcagccagat ccgcgatctt gaaaactgtg tcggtgttcc
    31141 gttgctggtg agggataagc gcaaagtcgc gttgacggcg gcgggagaat gttttctcca
    31201 ggacgcgctg gcaatcctcg aacaagcgga aaatgccaaa ttacgggcgc ggaaaattgt
    31261 tcaggaagac agacaattaa ccattggctt tgtgccatcg gcggaagtga atttactgcc
    31321 aaaagtatta ccgatgtttc gtctcagaca gccagacacc ttaattgagc tggtgagttt
    31381 aatcaccacg gaacaggaag aaaaaattcg ccgtggtgaa ctcgacgtcg gcttaatgcg
    31441 ccatcctgtt tatagcccgg agattgatta tctggagctt tttgacgaac cattagtggt
    31501 ggtgttaccg gttgatcacc ctttagcaca tgaaaaagag atcactgccg cgcaactgga
    31561 tggcgtgaat ttcgtcagta ctgatccagc gtattccggt tcgcttgcgc cgatcgttaa
    31621 agcgtggttt gcgcaagaaa acagccagcc aaatatcgtc caggtggcaa cgaatattct
    31681 ggtgaccatg aatctggtgg ggatggggct gggcgtcact ttgatacccg gttatatgaa
    31741 taattttaat accggccagg tggtatttcg acccattgcc ggaaatgtgc ctgccattgc
    31801 tttactgatg gcgtggaaga aaggggagat gaagccggca ttacgcgatt tcatcgccat
    31861 tgtgcaggaa cgtttggcaa gcgtaacggc ataattacgc attatttttg caaaacgcgt
    31921 cgcggatcgc agttttttag tgcttgtgaa ctggcgttac agacatcggg aagcaggctc
    31981 gaatattctt atttttcaac ttgcaccaac attcgattaa ccccagacta acgcctcctg
    32041 acgggaggga ctcatggttt tgcaatccac gcgctggttg gcgctcggct atttcacata
    32101 cttttttagt tacggcattt ttctaccttt ctggagcgtt tggcttaagg ggattggttt
    32161 aacgccagaa accatcggcc tgttattggg ggcaggtctg gttgcccgtt ttctcgggag
    32221 cttgctcatc gcgccccgcg tcagcgatcc ttctcgcctg atttccgcct tgcgcgtgct
    32281 ggcactgctg acacttctct ttgctgtcgc cttctgggcg ggggcgcacg tagcgtggct
    32341 gatgctggtg atgattggct ttaacctctt tttctcaccg ctggtaccgt tgaccgatgc
    32401 actggcgaat acgtggcaaa agcagttccc gcttgattac ggcaaagtgc gattgtgggg
    32461 ctcggtggcg tttgtcattg gctcgtcgct gacgggcaaa ctggtcacta tgtttgatta
    32521 tcgggtgatc ctcgcgctgt tgacgttggg cgtggcatcc atgctgctcg gctttctcat
    32581 ccgtccgacg attcagccac aaggggcaag ccgccagcag gagagcaccg gttggtctgc
    32641 gtggttggcg ttggttcgcc agaactggcg ctttctggcc tgcgtttgtt tattgcaggg
    32701 ggcgcatgcg gcctattacg gttttagcgc catttactgg caggctgctg gctactcggc
    32761 ctcggcggtg ggctatttgt ggtcgctggg cgtggtggcg gaagtcatta tctttgcgct
    32821 gagtaataaa cttttccgcc gttgtagtgc acgcgatatg ctgttgatct cagcgatttg
    32881 cggcgtagtg cgctggggca ttatgggagc aactacggcg ttgccgtggt tgatagtggt
    32941 gcaaattctg cattgtggca ccttcacggt ctgccacctg gccgccatgc gctatattgc
    33001 cgctcgccag ggtagcgaag tcatccgttt acaggcggtt tactctgccg tcgcgatggg
    33061 cggcagtatt gcgatcatga ccgttttcgc cggtttcctg tatcaatatc tgggccacgg
    33121 cgtgttctgg gtgatggcgc tggtggcgct tccggcaatg tttttgcgcc cgaaagttgt
    33181 tccctcatgc tgattcgagc attttgcgga tctgctcctg ttgatgtgtt gtcaacgtca
    33241 ggtccgtata gatcagcggt ggtgaaaaga gcggtaacgg catggtataa ggagcaataa
    33301 tcagtgccgc gctgcgcgga gcgcctgtct gcaaaaatgc ctttaccgac atatgtttaa
    33361 tattgagcgg cagtaacgtt agttcgcgta gctgctgctc aatctgcgct tctcgctcgc
    33421 tatcattccc ggtcagtaga ataatctgtt tctcatgcag gtcgttttcc tgcattagcc
    33481 aggcaccaaa aatcaccgcg accagaccgc tttcctcatc agaaagatgg acgccgtatt
    33541 cactttcaaa tccggccagc gccgcgcggg tggtgcgcac caggcgtgga tacaggcggg
    33601 cgaactcttc cggcagggtg ttatcaatac cgatggcaaa caaactgcga tttaacgcct
    33661 gggcgaggtg ggtataaagc tgatcgcata acccctgttc atcataaaaa cgaacatttc
    33721 ccagctcacg aaaatggttt accagacgtt tgatagactt acgcagttgt ctgtcccgct
    33781 gatgcgcatc ccgcagtgga tcgggaaccc gcaacatgga aaaaagtaat gccataaaca
    33841 gtggctcatc aggcggtaca ggctggagcg cccgacgctg ccagtggcgt ccaatttcct
    33901 gcgctacctg aaattcaagg caggattccg cccagcgacg ttggagcgga ttaaactgag
    33961 gcgtaattcc agcgtggtgt tgcagcaaac aatattgcag atacagacac aggaaatgaa
    34021 tatcgcgcgt ttcaaagcgt ttttgcagcc gtcgggagca gagattcact aacgcttgta
    34081 gattggtatc gtcgtaaaag ttacgcgcaa ttccgcgccg cttcagttca ctcttcaggg
    34141 cgggggtgaa atggctggta ataaatgacg gacacagacg gaaaccacgt cgtagccagt
    34201 gaaataaaca caaacgttgg ttaagcactg taccttcaac ccggtagcta ccgtcataac
    34261 cagtagtcag tgtgagttga tgatagcgca ggatctcccg tcctgtttcg ctgatatcaa
    34321 gactggcaat atcatcatcc acaccattaa gctcgctgaa agttgccgtg gtggcggtca
    34381 accccggctg aaagagcgtc agcaagatct ggcagcggcg ctggggagcc gaaaggacag
    34441 atggtggagc aagcgtaggc atcatctgta gatctcccat aagcataatt gataagaata
    34501 gctaaagttt gctcagggaa atggcgaagg gaatgctaat catcagaaat gttggcaagc
    34561 gtcacagaat ttttctcatc ttaaactgtt ctgaccagtg gattagcatg tcttaaggtg
    34621 gttttgtgaa tgattatcgt gctagcgatc atgggttagt tctgcggatt caacgcgcta
    34681 aggataaaat ccaccatctg ttcgcgataa acatcgccgt gacgatcgga aaaagcatca
    34741 atgtgttcgc catccgggat taggatcaac cgttttggct cttttgccag gctatacaac
    34801 ttttcgctgt gctgccatgg gataacgtga tcagctttac cgtgaatcag taaaagcggg
    34861 atcgggctga cgctggcgat gtaattttcg ccgctgtaac tctcatcaag taagtagcca
    34921 ctgccgggga tcatctggtt ggcgatggtc gcataagagg caaatgtgga gtcgaggatc
    34981 accgcacgta tgccttcacg atcaccctga ccaataacat ccaaaatatt cgccccgcca
    35041 atactctgcc cgaacagcac cagccgttgt gggtttacat cactgcgatg gcgcaccaca
    35101 ttgatggcac tttgcgtatc gtccagcaat ccggcctggg acggcgtgcc ttttgattta
    35161 ccaaacccgc gataatcaaa cataaaaacg ttgaaattac gctcgggtaa ccaactgacc
    35221 agcggccagt gggcggacat atttccggca ttgccgtgag catgaataat ggtggcaatg
    35281 gccttttcag cggggccagt tgcagaaggg ataaaccaac catgtaggcg agtgccatct
    35341 ttagcggtga attctactga ttcggctgac cagggatcgg ggccgtagat tttgtcgtca
    35401 gggtaataga aaaaattgat ggcgatgcgc ggtattaaat aaatgcagaa ggcaacaaca
    35461 aagagaataa acagaatttt gagaacgcgt ttgttcactg gcagcgccat aacctttcct
    35521 tgcgacttct ttatcatcca ttaatcgggg aaagatggtg ggagagtggc gaaagaaagt
    35581 aaagcggtga ggaagtattt cggaatagcg cctgaaaggc gagggcgggt gagtgacatc
    35641 acccgccaga gtcattaacg cttcagggcg tcgcttaact cgtcacgcat gttcgccagc
    35701 atggctttaa caacgcgcgg gttaccagca acgatgttac cggtcagcat gtagttatga
    35761 ccaccggtga agtcgctgac gatgccgccc gcttcacgaa ccagcagctc gcctgccgcg
    35821 aaatcccacg gacgcagacc gatttcaaag aaaccgtcaa cgcgacccgc agcaacgtaa
    35881 gccagatcca gcgccgcaga accggtacga cggaagtctg cacattcgtt gaacagtttg
    35941 ccgacgatgt tgatgtaggt agtggcgtac tgttttgctt tgaacgggaa gccggtcgcc
    36001 agaatagtac cgtcgagatc gcgagcggtg ctgccgcgca gacggtagcc gttcagctgt
    36061 gcgccctgac cgcgagtggc ggtgaacagt tcgttacgca taggatcgta aaccacagca
    36121 acttcagtgc ggcctttgat acgaacggcg atagataccg cgaagtgcgg cagacgtttg
    36181 ataaagttgg tagtgccatc cagtggatcg ataacccatt gaacatcctg atcagtacct
    36241 tcaagttcac cgctttcttc ggtgatgatg gtgtgctgtg ggtaagattt acgaatcgtg
    36301 tcgataatca ccgcttcggc agctttatct acgttggtca cgaagtcgtt actgcctttc
    36361 tggctcgctt ctacagcgtc cggggtttca tagtttttgg caattaaatt acccgccttg
    36421 cgcgctgcgc gcacggcgat gttcagcatc ggatgcatcg gtctctctca ctggatgtta
    36481 aagaacggga aaacggcgca gagtatagca gcgagatcgg catctgtctt cgttttatga
    36541 taagatgcgt gaataatctt tagatgaaga aagacaatgc tgcaaaatat tcgaattgtg
    36601 ctggtggaga cgtcacacac cggcaatatg gggtctgttg cccgtgccat gaaaacaatg
    36661 ggattaacca atctgtggct ggttaatcca ctggtgaaac ctgactccca ggcgattgcc
    36721 ctggcagcag gggccagcga tgtgattggt aatgctcaca tcgtcgatac gctcgacgaa
    36781 gcgttagccg gttgtagcct ggtggtgggc accagcgcac gctcccgcac gctgccgtgg
    36841 ccgatgctcg acccgcgcga atgcggtctg aaaagcgtcg ctgaagcggc aaataccccg
    36901 gtggcactgg tgtttggtcg cgagcgcgtc ggcttgacca atgaagagct gcagaaatgc
    36961 cattatcatg tcgcgattgc ggctaacccg gaatacagtt cgctgaacct ggcgatggcg
    37021 gttcaggtta ttgcctatga agtgcgcatg gcctggctgg caactcagga aaatggcgag
    37081 caggtcgagc atgaagagac gccgtatccg ctggtcgatg atctggagcg tttttatggt
    37141 catctggaac aaacgctgct ggcaaccggt tttattcgtg aaaaccatcc ggggcaggtg
    37201 atgaataaat tgcgccgtct gtttacccgt gcgcgcccgg aaagccagga attgaatatc
    37261 ctgcgcggga ttctggcttc tatagagcag cagaataaag gtaacaaggc cgaataatag
    37321 tggttgcctg atgcgacgct taacgcgtct tatcaggcct gcagtgcaca gagcccctgg
    37381 gcggataagg cgttcacgtc gcatccgaca acaggtacaa acgccacgat aaaaaaatgg
    37441 cactgaaggt taaatacccg actaaatcag tcaagtaaat agttgaccaa tttactcggg
    37501 aatgtcagac ttgaccctgc tatgcaatac ccccactttt acaataaaaa accccgggca
    37561 ggggcgagtt tgaggtgaag taagacatga gactgacatc taaagggcgc tatgccgtga
    37621 ccgcaatgct tgacgttgcg ctcaactctg aagcgggccc ggtaccgttg gctgatattt
    37681 ccgaacgtca gggaatttcc ctttcttatc tggaacaact gttttcccgt ctgcgtaaaa
    37741 atggtctggt ttccagcgta cgtggaccag gcggtggtta tctgttaggc aaagatgcca
    37801 gcagcatcgc cgttggcgaa gtgattagcg ccgttgacga atctgtagat gccacccgtt
    37861 gtcagggtaa aggcggctgc cagggcggcg ataaatgcct gacccacgcg ctgtggcgtg
    37921 atttgagcga ccgtcttacc ggttttctca ataacattac tttaggcgaa ctggttaata
    37981 accaggaagt gctggatgtg tctggtcgtc agcatactca cgacgcgcca cgcacccgca
    38041 cacaagacgc gatcgacgtt aagttacgcg cttaataaaa agaattcaga atcaggccgg
    38101 agtgctaaac actccgtaaa cacggtcgta catccagccg gtagcctggt tccttgcatt
    38161 gagtgatgta cggagtttat agagcaatga aattaccgat ttatctcgac tactccgcaa
    38221 ccacgccggt ggacccgcgt gttgccgaga aaatgatgca gtttatgacg atggacggaa
    38281 cctttggtaa cccggcctcc cgttctcacc gtttcggctg gcaggctgaa gaagcggttg
    38341 atatcgcccg taatcagatt gccgatctgg tcggcgctga cccgcgtgaa atcgtcttta
    38401 cctctggtgc aaccgaatct gacaacctgg cgatcaaagg tgcagccaac ttttatcaga
    38461 aaaaaggcaa gcacatcatc accagtaaaa ccgaacataa agcggtactg gatacctgcc
    38521 gtcagctgga gcgcgaaggt tttgaagtca cttacctggc accgcagcgt aatggcatta
    38581 tcgacctgaa agaactcgaa gcagcgatgc gtgacgacac catccttgtt tccatcatgc
    38641 acgtaaataa cgaaatcggc gtggtgcagg atatcgcggc tatcggcgaa atgtgccgtg
    38701 ctcgtggcat tatctatcac gttgatgcaa cccagagcgt gggcaaactg cctatcgatc
    38761 tgagccagtt gaaagttgac ctgatgtctt tctccggtca caaaatctat ggcccgaaag
    38821 gtattggtgc gctgtatgtg cgtcgtaaac cgcgcgtacg tatcgaagca caaatgcatg
    38881 gcggcggtca cgagcgcggt atgcgttccg gcactctgcc tgttcaccag atcgtcggta
    38941 tgggcgaagc ctatcgcatc gcaaaagaag agatggcgac cgagatggaa cgtctgcgcg
    39001 gcctgcgtaa ccgtctgtgg aacggcatca aagatatcga agaagtttac ctgaacggtg
    39061 acctggaaca cggtgcgccg aacattctca acgtcagctt caactacgtt gaaggtgagt
    39121 cgctgattat ggcgctgaaa gacctcgcgg tttcttcagg ttccgcctgt acgtcagcaa
    39181 gcctcgaacc gtcctacgtg ctgcgcgcgc tggggctgaa cgacgagctg gcacatagct
    39241 ctatccgttt ctctttaggt cgttttacta ctgaagaaga gatcgactac accatcgagt
    39301 tagttcgtaa atccatcggt cgtctgcgtg acctttctcc gctgtgggaa atgtacaagc
    39361 agggcgtgga tctgaacagc atcgaatggg ctcatcatta atcggtatcg gaatcaggag
    39421 aatttataat ggcttacagc gaaaaagtta tcgaccatta cgagaatccg cgtaacgtgg
    39481 gttcctttga caacaacgac gagaacgtcg gcagcggcat ggtgggtgca ccggcctgtg
    39541 gcgacgtgat gaagttgcag attaaagtca acgatgaagg tatcattgaa gacgcgcgtt
    39601 ttaaaactta cggctgcggt tctgctatcg cttccagctc cctggtcacc gaatgggtga
    39661 aagggaagtc tctcgacgaa gcgcaggcga tcaaaaacac cgatattgct gaagaacttg
    39721 aactgccgcc ggtgaaaatt cactgttcta ttctggcaga agacgcgatc aaagccgcca
    39781 ttgcggacta taaaagcaaa cgtgaagcaa aataagagtt gaggtttggt tatgtcgatt
    39841 acactgagcg acagtgcagc agcgcgagta aatacctttc tggctaaccg cggcaaaggg
    39901 tttggcctgc gtctgggcgt gagaacctcc ggatgttcag gtatggctta tgtactggaa
    39961 tttgttgacg aaccgacgcc ggaagacatc gtgtttgaag acaaaggcgt gaaagtcgtg
    40021 gtcgatggca aaagcctgca atttctggac ggtacgcagc tggacttcgt aaaagaaggc
    40081 ctgaacgaag ggtttaaatt caccaacccg aacgtcaaag atgagtgtgg ttgcggcgaa
    40141 agcttccacg tttgatgcgc ataccgataa ccccaccgtg gtcgcctgcg cgtggggttt
    40201 gttttacctg attcgccgtt atcgcggcgg atcgcagccc tgagaatgtt atggattact
    40261 tcaccctctt tggcttgcct gcctgttatc aactcgatac ccaggcgctg agcctgcgtt
    40321 ttcaggatct acaacgtcag tatcatcctg ataaattcgc cagcggaagc caggcggaac
    40381 aactcgccgc cgtacagcaa tctgcaacca ttaaccaggc ctggcaaacg ctgcgtcatc
    40441 cgttaatgcg cgcggaatat ttgctttctt tgcacggttt tgatcttgcc agcgagcagc
    40501 atactgtgcg cgacaccgcg ttcctgatgg aacagctgga actgcgcgaa gaactggacg
    40561 agatcgaaca ggcgaaagat gaagcgcggc tggaaagctt tatcaaacgt gtgaaaaaga
    40621 tgtttgatac ccgccatcag ttgatggttg aacagttaga caacgagacg tgggacgcgg
    40681 cggcggatac cgtgcgtaag ctgcgttttc tcgataaact gcgaagcagt gccgaacaac
    40741 tcgaagaaaa actgctcgat ttttaatttc tggaagctaa acatggcctt attacaaatt
    40801 agtgaacctg gtttgagtgc tgcgccgcat cagcgtcgtc tggcggccgg tatcgacctt
    40861 ggcacaacca actcgctggt ggcgacagtg cgcagcggtc aggccgaaac gttagccgac
    40921 catgaaggcc gtcacctgct gccgtctgtt gttcactatc aacagcaagg gcattcggtg
    40981 ggttatgatg cgcgtactaa cgcagcgctc gataccgcca acaccattag ttctgttaaa
    41041 cgcctgatgg ggcgctcgct ggctgatatc cagcaacgct atccacatct gccttatcaa
    41101 ttccaggcca gcgaaaacgg cctgccgatg attgaaacgg cggcggggct gctgaacccg
    41161 gtgcgcgttt ctgcggacat cctcaaagca ctggcggcgc gggcaactga agccctggca
    41221 ggcgagctgg atggtgtagt tatcaccgtt ccggcgtact ttgacgatgc ccagcgtcag
    41281 ggcaccaaag acgcggcgcg tctggcgggg ttgcatgttc tgcgcttact taacgaaccg
    41341 accgctgcgg ctatcgccta cgggctggat tccggtcagg aaggcgtgat cgccgtttat
    41401 gacctcggcg gcgggacgtt tgatatttcc attctgcgct taagtcgcgg cgtgtttgaa
    41461 gtgctggcga ccggcggtga ttccgcgctc ggcggcgatg atttcgacca tctgctggcg
    41521 gattacattc gcgagcaggc gggcattcct gatcgtagcg ataaccgcgt tcagcgtgaa
    41581 ctgctggatg ccgccattgc agccaaaatc gcgctgagcg atgcggactc cgtgactgtg
    41641 aatgttgcgg gctggcaggg cgaaatcagc cgtgaacaat tcaacgaact gatcgcgcca
    41701 ctggtaaaac gaaccttgct ggcttgtcgt cgcgcgctga aagacgctgg cgtagaagct
    41761 gatgaagtgc tggaagtggt gatggttggc ggttctactc gcgtgccgct agtgcgtgaa
    41821 cgagtaggcg aatttttcgg tcgtccaccg ctgacttcta tcgacccgga taaagtcgtc
    41881 gctattggcg cggcgattca ggcggatatt ctggtgggta acaagccgga cagcgaaatg
    41941 ctgttgctgg atgtgatccc gctgtcgctg ggcctcgaaa cgatgggcgg cctggtggag
    42001 aaagtgattc cgcgtaatac cactattccg gtggcgcgcg ctcaggattt caccaccttt
    42061 aaagatggtc agacggcgat gtctatccat gtaatgcagg gtgagcgcga actggtgcag
    42121 gactgccgct cgctggcgcg ttttgcgctg cgtggtattc cggcgttacc ggctggcggc
    42181 gcgcatattc gcgtgacgtt ccaggtcgat gccgacggtc ttttgagcgt gacggcgatg
    42241 gagaaatcca ccggcgttga ggcatctatt caggtcaaac cgtcctacgg tctgaccgac
    42301 agcgaaatcg cctcgatgat caaagactca atgagctatg ccgagcagga cgtaaaagcc
    42361 cgtatgctgg cagaacaaaa agtagaagcg gcgcgtgtgc tggaaagttt gcacggcgcg
    42421 ctggctgctg atgccgcgct gttaagcacc gcagagcgtc aggtcattga cgatgctgcc
    42481 gctcacctga gtgaagtggc gcagggcgat gatgttgacg ccatcgaaca agcgattaaa
    42541 aacgtagaca aacaaaccca ggatttcgcc gctcgccgca tggaccagtc ggttcgtcgt
    42601 gcgctgaaag gccattccgt ggacgaggtt taatatgcca aagattgtta ttttgcctca
    42661 tcaggatctc tgccctgatg gcgctgttct ggaagcgaat agtggtgaaa ccattctcga
    42721 cgttgcgctg cgtaacggta tcgagattga acacgcctgt gaaaaatcct gtgcttgcac
    42781 cacctgccac tgcatcgttc gcgaaggttt tgactcgctg ccggaaagct cagagcagga
    42841 agacgacatg ctggacaaag cctggggact ggagccggaa agccgtttaa gctgccaggc
    42901 gcgcgttacc gacgaagatt tagtggtcga aatcccgcgt tacactatca accatgcgcg
    42961 tgagcattaa cagaggttag tatgggactt aagtggaccg atagccgcga aattggcgaa
    43021 gcactgtacg atgcgtatcc cgatcttgat ccgaaaacgg ttcgattcac cgatatgcat
    43081 cagtggattt gcgatctgga agatttcgac gacgacccgc aggcatcgaa cgagaaaatc
    43141 ctcgaagcga ttttgttggt ctggctggac gaagccgagt gaatgaacgg gctgcctggg
    43201 ggtagcccgt tttgctgtta aacgaataag gataactaaa atgacagaag cgatgaaaat
    43261 taccctttct acccaacctg ccgacgcgcg ctggggagaa aaagcgactt acagcattaa
    43321 taatgatggc attaccctgc atttgaacgg gacagacgat ctggggctga tccagcgtgc
    43381 ggcgcgcaag attgacggtc tgggcatcaa gcatgttcag ttaagcggtg aagactggga
    43441 tgcggatcgc tgctgggcat tctggcaagg ttacaaagcc ccgaaaggca cacgtaaagt
    43501 ggagtggccg gatctggacg atgcccaacg gcaggaactg gataaccgcc tgatgatcat
    43561 cgactgggtg cgtgacacca tcaacgcacc ggcggaagaa ctggggccat cgcaactggc
    43621 acagcgtgct gttgatctga tcagcaacgt cgcgggcgat cgtgtgactt accggatcac
    43681 caaaggcgaa gatctgcgtg atcaaggtta tatggggctg cacacggtcg ggcgcggttc
    43741 agaacgttcc ccggtattgc tggcgctgga ttacaaccca acaggcgata aagaagcgcc
    43801 agtgtacgcg tgcctggttg gtaaaggcat cacctttgat tccggcggct acagtatcaa
    43861 acagaccgca tttatggact cgatgaagtc ggacatgggc ggcgcggcaa cggttaccgg
    43921 agcgctggcg tttgccatta cgcgcggact gaacaagcgc gtgaagctgt tcctctgctg
    43981 tgcggataac ctgatcagcg gcaatgcgtt caagctgggc gatatcatta cctatcgcaa
    44041 cggtaaaaaa gttgaagtga tgaacaccga tgcggaaggg cgtctggtgc tggccgatgg
    44101 cctgattgat gccagtgcgc agaaaccgga actgatcatt gatgcggcga ccctcaccgg
    44161 ggcggcgaaa accgcgctgg gtaatgatta tcacgcgctg ttcagttttg acgatgcgct
    44221 tgccgctcgc ttgctggcga gtgccgcgca ggagaatgaa ccattctggc gtctgccgct
    44281 ggcggagttc caccgcagcc agctgccgtc taactttgcc gaactgaaca ataccggaag
    44341 cgcggcgtat ccggcaggtg caagcacggc ggcgggcttc ctgtcgcact ttgttgagaa
    44401 ctatcagcaa ggctggctgc atatcgactg ctcggcgact taccgtaaag cgccggttga
    44461 acagtggtct gcgggcgcga ccgggctagg tgtgcgcacg attgctaatc tgttaacggc
    44521 gtaaacgtct tatttggcct acaaaattgc gaaaatccaa tagattgaga ttcccttgta
    44581 ggcctgataa gcgtagcgca tcaggcactt gtgccccgaa cgttgaatga attgtggttc
    44641 ttatgtccga aacaaaaaac gaacttgaag acctgctgga aaaagcagca accgaaccgg
    44701 cgcaccgccc ggcctttttc cgtactctac tggaatccac cgtctgggtg ccgggtacgg
    44761 cggcgcaggg cgaggctgtg gttgaagata gcgcgcttga tttacagcac tgggaaaaag
    44821 aagacggcac cagcgtcatt ccttttttta cctcgttaga agcacttcaa caggcggttg
    44881 aagacgaaca ggcatttgtc gtaatgcccg ttcgcacatt gtttgagatg acccttggcg
    44941 aaacgctctt ccttaatgcc aaactgccaa ccggtaaaga atttatgccg cgtgaaatca
    45001 gtttgctgat tggtgaagaa ggaaatccgc tgagcagcca ggaagtactg gaaggcggtg
    45061 aatcgctgat attatcggaa gtcgcagagc caccagcaca aatgattgat tcactcacca
    45121 ccttatttaa aactattaag ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg
    45181 aagaggcaca gcctaattta cttattggca ttgaagccga tggtgatatc gaagaaatta
    45241 ttcaggcggc gggaagtgta gcgaccgata cattacctgg cgatgaaccc atcgatattt
    45301 gtcaggtgaa aaaaggggaa aaaggaatta gccactttat taccgaacat attgcgccat
    45361 tctatgaacg tcgctggggt ggttttttgc gtgactttaa acagaaccgg ataatctaaa
    45421 atatgccgcc ccaaagggcg gcattcgggc ttacttgctg ctctgaaggt tgttaaccgc
    45481 cagtaaattg ttgccggaga gactccggtt attatttacc tcgcttaaca gcgcaccaga
    45541 aacaataagt acgatacaaa agcaccgtat cttcattgtg cggtccatgc gaagaccgca
    45601 tcacgacagc accactggcg atattgccgc gatagcaagc gctgagacgc gcttgctcta
    45661 acggtcgcgg aacatccttt ccttaagcca gcgcccgctt gcgagacggc ggaaaggtcc
    45721 gcagtggata atacactaac agaacaataa taactctctt ttacttgaca aaaagagagt
    45781 tactggcgag tattgtgtcc gctgagacgc gcttgcatta ctacggtaat acaatctgct
    45841 ggaaaggccg cgaaccaaac cagcaacaaa taaccgcctg attttggcgg ttttttgttt
    45901 tttaaaggaa agtgaattta catgtttaag taatatcttt taccggttag tatttttaat
    45961 tttgagcagg atttgtttat atttgtctga attcggacta ataagccagg ttattttatt
    46021 accgaacacg gcggaaaggg ccggtgtcga taatacgcta aataaacaat gattactctc
    46081 tttcgcttga caaaaagaga gttactggcg agtattgctt tgctgtgaag cacctgcgtt
    46141 gctcatgcaa cacaagccaa tctgctggaa aggccgcgaa ccagaccagc aataaaaaac
    46201 cgccagattc ggcggttttt tattgcctgt taaaagctta tttctctggc tcaactggta
    46261 aatctgctcg tgcgccccat tcactccatg cgccgtcgta cagtttcacg tttggcacat
    46321 caagtgtcgc gagtgccaac aaaaccacgg ctgccgttac accagagccg cagctgacga
    46381 taataggttt gtcataactt acgccgcgac caaaaaatat cgcatccagt tcatcggtcg
    46441 tttttagttc accttcgcgc accagttccg tccacggaac atttagtgca ccgggaatat
    46501 gtccgcgacg taaacctggg cgaggttcat caacttctgc gttaaaacgt gcagccgggc
    46561 gggcatcgat aatttgcgcc gtattttcat ggcttgccaa taacacatcg gttactttca
    46621 ccacggcttc aggattaaac gcggcgttaa actctccttc cggcagctct actgcgcctt
    46681 cttctaatag cagatcatca cgctgccagc ctgcaagtcc accacccaga atcgacactt
    46741 tctctacacc aaaggtgcgc agcatccacc atgctcgtgg ggctgagaaa agattacctt
    46801 cgtcatagac aatcaggtgc ttatcctggt taacgcctaa ttcacgcatc gccacggcga
    46861 acgtttccgg gcgcggcagc atgtgcggaa gcggggaagt gtgatcagaa agcgcttcta
    46921 tatcaaaaaa cactgcgccg ggaatatgtc cattcagata ctcctgagca acgttacgat
    46981 cttcctgtcc aggcgacgcc atgcgggcat cgataatctg aatttccggg tcatcaatat
    47041 gttcggcgag ccagtcggct cctacaaacc atgtcgtgga cataggcatc tccatttttg
    47101 ccggggttag gctaaattgt cggatgttta gtgaaaggcg acaagcaaaa gaggctgatt
    47161 tggctgtctg agccacaatt ctcactaaaa ttcggattgt atgggcgtga tactgaatta
    47221 cataacgtaa attctcacgc ataataatta ttcgacgttg ctaacgggcc attgtggcag
    47281 ggatgaaaaa tgaaaaagtt acgcgtagcc gcctgcatgc taatgctggc gctggcaggg
    47341 tgcgacaaca acgataacgc gccaacagcg gtgaaaaaag atgcgccttc tgaagttact
    47401 aaagcggcct cttcagaaaa cgtgagttca gcaaaacttt ccgcgccaga gcgacaaaaa
    47461 ctggcccaac agagtgccgg taaggcgctg acattgctgg atctctctga agttcaactt
    47521 gatggcgcag ccacgctggt gctgacattc tccatccctc tcgacccgga tcaggaattc
    47581 tcacgcgtta tccatgtcgt cgataagaaa agcggcaaag tggatggtgc ctgggagctg
    47641 tcagataatc ttaaagagtt gcgtttacgc cacctcgaac cgaaacgtga tttgatcgtc
    47701 actattggca aggaggtcaa agcactcaac aacgcaacct tcagtaaaga ttacgaaaaa
    47761 actataacta cccgagatat ccaacccagc gtcggttttg ccagccgtgg ctcgttgtta
    47821 cctggtaaag ttattgaagg gctgccggta atggcgctca acgttaacaa tgtcgatgtt
    47881 aacttcttcc gtgttaagcc agaatctctg cctgcattca ttagccaatg ggaataccgc
    47941 aattcgctgg cgaactggca gtctgacaaa ctgctgcaga tggcggatct ggtttatacc
    48001 ggacggtttg atctcaattc tgcgcgtaac acccgtgaaa aattattgct gccgctgggc
    48061 gatatcaaac cgcttcagca ggcaggcgtg tatctggctg tgatgaatca ggctggacgt
    48121 tacgattaca gtaatcccgc gacgctgttt acgttaagtg atatcggcgt ttcagctcac
    48181 cgttatcaca atcgtctgga tatctttacc caaagcctgg aaaacggcgc ggcccagcaa
    48241 ggagttgaag tctcattatt aaatgagaaa gggcagactc tgactcaggc aaccagtgat
    48301 gcccaggggc atgtgcagct ggaaaatgat aaaaacgcgg cattactgtt ggcgcgtaaa
    48361 gacggtcaga caacgctact cgatttaaaa cttccggcgc tggacttagc agaatttaac
    48421 attgctggcg cgccaggcta tagcaaacag tttttcatgt ttggcccgcg cgatctttat
    48481 cgccctggtg aaacagtcat cctcaatggt ttattgcgtg atgcagatgg taaagcattg
    48541 cccgatcaac ccatcaagtt agacgtgatt aaacccgacg ggcaggtact caggagcgtc
    48601 gttagtcagc cggagaatgg cctctaccac tttacctggc cactcgatag caatgcggca
    48661 accggtatgt ggcatattcg cgctaacacg ggcgataacc agtatcggat gtgggatttc
    48721 cacgtcgaag attttatgcc agagcgcatg gcgctgaatc tgaccggtga gaaaaccccg
    48781 ctaacgccga aagatgaagt gagattctcc gtggtggggt actacctgta tggtgcgcct
    48841 gctaatggta atactttgca agggcaactt ttcctgcgcc cactgcgtga agctgtgtca
    48901 gccttacctg gtttcgaatt cggcgatata gctgccgaaa atctttcccg cacgctggat
    48961 gaagttcagt tgacgctgga tgataaaggg cgtggcgaag tttctacaga aagccagtgg
    49021 aaggaaacgc attccccatt acaggttatt ttccagggta gtttgttgga atcgggcggt
    49081 cgcccggtga cgcgccgcgc tgagcaggct atctggcctg ccgatgcatt gccggggatc
    49141 cgtccgcagt tcgcctcgaa atcggtttac gattatcgca ctgacagcac ggtgaaacag
    49201 cccattgttg atgaaggcag taacgccgct tttgacatcg tttatagcga tgcgcaaggc
    49261 gtaaaaaaag ccgtgtcggg cttgcaggtg cgcctgattc gcgaacgccg cgattactac
    49321 tggaactggt cagaagatga aggctggcag tcacagtttg atcaaaaaga tctgatcgaa
    49381 aatgaacaaa ctctggatct gaaagcggac gaaaccggca aggtcagttt cccggtagag
    49441 tggggcgctt atcgtctgga agtcaaagcg ccgaatgaag cggtcagtag tgtgcgtttc
    49501 tgggctggct atagctggca ggacaacagc gacgggagcg gtgccgtgcg acccgaccgt
    49561 gtcacgctga aactggataa agccagctat cgccctggcg ataccattaa gttgcatatt
    49621 gccgcgccaa cggcgggtaa aggttatgcg atggtcgagt ccagtgaagg gccgctgtgg
    49681 tggcaagaga ttgatgttcc ggctcaaggg ctggatctga cgattccggt cgataaaacc
    49741 tggaatcgtc atgatctgta tttaagtacg ctggtggtac gtcctggcga taaatctcgc
    49801 tccgcgacgc caaaacgcgc ggttggggtg ttgcatctgc cgcttggtga tgaaaaccgt
    49861 cgcctcgatc tggcgctgga aacccctacc aaaatgcgcc ccaatcagcc attaaccgtg
    49921 aaaattaaag ccagcaataa aaatggtgag atgccaaaac aggtgaatgt gctggtgtct
    49981 gccgttgata gcggtgtgct gaatattact gattacgtca cgccagatcc gtggcaggcg
    50041 ttctttggtc agaaacgcta tggcgcagac atttacgata tttacggtca ggtcattgaa
    50101 ggtcaggggc gtctggcagc tctgcgtttt ggtggtgatg gtgatgagct gaaacgtggt
    50161 ggtaaaccgc cggtcaatca cgtcaatatt gtcgcgcagc aggcgctgcc ggtaacgctc
    50221 aacgaacagg gcgaaggctc ggttacactg ccaattggcg attttaacgg tgaattgcgc
    50281 gtcatggcgc aagcctggac ggcagatgac ttcggaagca acgaaagcaa agtgattgtt
    50341 gctgcaccgg tgattgctga actgaacatg ccacgcttta tggcgagtgg cgatacctcg
    50401 cgcctgacgc tggatatcac taatcttacc gataaaccgc aaaaacttaa tgttgccctg
    50461 accgccagtg gtttgcttga actggtcagc gattcacccg cagccgttga attagcgcca
    50521 ggggtgcgta ctacgctgtt tatcccggtg cgtgcattgc cgggttatgg cgatggagac
    50581 attcaggcca ccattagcgg gttagcgtta cccggggaaa ccgttgccga tcagcataag
    50641 cagtggaaaa tcggcgtccg tccggcattc ccggcacaaa cggtcaatta cggtacggcg
    50701 ttacagcctg gtgaaacctg ggcgctcccg gctgatggat tgcaaaactt ctcgcctgtt
    50761 acgctggaag ggcaattgtt gttgagcggc aaaccaccgc tgaacatcgc acgttatatc
    50821 aaagagttaa aagcgtatcc gtacggctgt ctcgagcaaa ccaccagcgg cctgttcccg
    50881 tcactttata ccaatgcagc ccaactgcag gcgttgggca tcaaaggcga cagtgatgag
    50941 aaacgccgtg catcggtcga tatcggcatt tcccgtttgc tgcaaatgca acgtgataac
    51001 ggcggcttta cgctgtggga taaaaacggt gacgaagagt actggctgac ggcttacgtg
    51061 atggatttcc tggtccgcgc aggcgaacag ggttacagcg taccgacaga cgccattaac
    51121 cggggtaatg agcgtctgct gcgctattta caagatccgg gcatgatgtc gatcccgtac
    51181 gctgataatc tcaaagccag taaattcgcc gtacagtctt acgctgcgct ggtgttggcc
    51241 cgtcagcaaa aagcaccact gggtgcgctg cgtgaaatct gggagcatcg tgcagatgcc
    51301 gcttctggtt taccgctgct gcaacttggc gttgcgctga aaaccatggg tgatgcaatg
    51361 cgtggtgaag aagcgattgt gctggcgctg aaaacgccgc gtaatagtga tgagcggata
    51421 tggctgggtg attacggtag tccactgcgc gacagcgcgt tgatgctctc attgctggaa
    51481 gagaacaaac tgctacccga tgagcagtac tccctgttga acacactttc gcagcaggcg
    51541 ttcggtgaac gctggctatc gacgcaggaa agtaacgcgt tgttcctggc tgcccgtact
    51601 cttcaggatt taccgggcaa atggcaggcg caaaccactt tctctgctga gccgctgaca
    51661 ggcgagaaag cgcaaaccag caatctgaat agcgatcaac ttgccacctt gcaggtgacc
    51721 aacagtggcg atcagccgtt atggctgcgt gtggatgcca gcggttatcc gcaatccgca
    51781 cctttatcgg cgagcaatgt gctgcaaatt gagcgtcata ttctgggtac tgacggtaag
    51841 agcaaatcgc tggactcgtt acgtagcggc gatctggtgc tggtgtggtt gcaggttaaa
    51901 gccagtaaca gtgtaccgga tgcgttagtc gtggatctgt tgcctgcggg gctggaactg
    51961 gaaaaccaga atctggcgaa cggcagcgcc agcctggagc aaagtggtgg tgaagtgcag
    52021 aacttgctga accagatgca gcaggcgagc attaagcaca tagagttccg tgacgatcgc
    52081 tttgtggcgg cggttgccgt tgatgaatat caaccggtaa cgctggtgta tctggcgcgg
    52141 gcggtgacgc cgggaacgta tcaggtgccg cagcctatgg tggaatcaat gtatgttcct
    52201 caatggcggg cgactggtgc ggctgacgat ctgttgatag tcagaccgta aatgcctcgc
    52261 ctgttaacca aacgcggctg ctggataatg ctggcagccg cgccttttat catcattctg
    52321 gcagcatggg cagcagataa actctggcct ctgccattgc aagaagtgaa tcctgcacgc
    52381 gtggtggtgg cgcaggatgg tacgccactc tggcgtttcg ccgatgctga cggtatctgg
    52441 cgttatccgg taacaatcga agatgtttct ccacgttacc ttgaagcgct gatcaattat
    52501 gaagatcgct ggttctggat gcatccgggg gtgaatccgt tctcggtggc gcgtgcagcc
    52561 tggcaagatc tcacgtcggg tcgggtcatt tccggcggca gcacgctcac catgcaggtg
    52621 gcgcgcctgc ttgatcctca ccccaaaacg tttggcggca aaattcgcca gctctggcgc
    52681 gcgttgcaac tggaatggca tctgtctaaa cgtgaaattc tgaccttgta tcttaaccgt
    52741 gccccgtttg gcggtacgtt gcaggggatt ggtgcggcaa gttgggctta cctcgggaaa
    52801 tcgcctgcga atttaagcta ttccgaggcg gcaatgctgg cggttttgcc gcaagcgccc
    52861 agccgtcttc gcccggatcg ttggccggag cgtgccgaag ccgcgcgtaa taaagtgctc
    52921 gaacggatgg ccgtgcaagg tgtgtggtcc cgtgagcggg taaaagagtc aagggaagaa
    52981 cccatctggc tggcaccccg acaaatgccg caactggcac tgctgttttc gcgcatgatg
    53041 ctcggtaaaa gcaaaagcga caaaatcgtt actacgctgg atgccggtct tcaacgacgt
    53101 ctggaagaac tggcgcaaaa ctggaaagga cgattgccac cgcgcagctc actggcgatg
    53161 atcgtggttg atcataccga tatgcgtgtt cgcggctggg tgggatcagt tgatctcaac
    53221 gatgattcac gctttggtca tgttgatatg gtcaatgcga tccgatcgcc aggatcggtg
    53281 ctcaaaccgt ttgtttatgg tctggcgctg gatgaaggct tgatccaccc ggcatcgctg
    53341 ttgcaagacg tccccagacg caccggtgat taccgacctg gcaactttga cagcggtttt
    53401 catggcccga tcagcatgag tgaggcgctg gtgcgatcgc tgaacttacc tgctgtgcag
    53461 gtgctggaag cctatggacc gaaacggttt gcggcaaagt tacgcaatgt tggattgccg
    53521 ttatatttgc ccaacggtgc tgcaccgaat ctttcgctca ttctcggcgg cgctggcgca
    53581 aaactggaag atatggcggc agcgtatacc gcgtttgctc gccacggcaa ggcaggcaaa
    53641 ttgcgcttac agcctgacga tccgctgctt gaacggcctt taatgtcgtc gggggctgcg
    53701 tggatcattc gccggattat ggctgatgaa gcgcaaccct tgccggatgg tgccttgccg
    53761 cgcgtcgccc cactggcatg gaaaacgggc accagctatg gctatcgtga tgcctgggcg
    53821 attggggtta acgctcgcta tgtcattggg atctggactg gcagaccgga cggcacgccc
    53881 gttgttggtc agtttggctt tgccagtgcc ataccgctgt tgaatcaggt taataatatc
    53941 ttactgtcgc gcagtgtgaa tttgccggaa gacccacgtc ctgactctgt aagtcgtggc
    54001 gttatctgct ggccgggcgg acagtctttg gcggaaggtg acggtaactg tcgccgccgc
    54061 ctggcaacct ggctgctgga cgggagccag ccgccaactc tattactgcc ggagcaggaa
    54121 ggcattaatg gcattcgttt ccccatctgg ctggatgaaa acggcaaacg tgttgccgct
    54181 gattgccctc aggcgcgaca agaaatgata aatgtctggc cgctaccgct ggaaccctgg
    54241 ctgcccgcat cagaacgtcg cgctgtgcgc ttgccaccgg cctcgacaat ttgcccgcca
    54301 tacggtcacg atgcacaact cccgctgcag ctaacaggtg tgcgcgatgg cgcgattatt
    54361 aaacgtttac cgggcgcagc ggaagcgact ttgccgttgc aatcaagtgg aggggcaggt
    54421 gaacgctggt ggtttctgaa cggcgaaccg ttaactgaac gtgggcgcaa cgtgaccctg
    54481 catttgacgg ataaaggcga ttatcaattg ctggtgatgg atgaggtggg gcaaatagcg
    54541 acagtgaaat ttgtcatgca atagtctgtt ttgtccctga ttgttgctta aactgggttt
    54601 attttaaaaa aatgttacct tcatcaatag tcaacggctc tgttgctcat tataatccgc
    54661 gccatctcgt acgctggtac agacaacaac agaacaattt acagaggtaa aaatggctat
    54721 tgaacgtact ttttccatca tcaaaccgaa cgcggtagca aaaaacgtga ttggtagtat
    54781 ctttgcgcgc tttgaagctg cagggttcaa aattgtcggc accaaaatgc tgcacctgac
    54841 cgttgaacag gctcgtggct tttatgctga acacgatgga aaaccgttct ttgatggtct
    54901 ggttgaattc atgacttctg gcccgatcgt tgtttccgtg ctggaaggtg aaaatgctgt
    54961 tcagcgtcac cgcgatctgc tgggcgcgac caatccggca aacgcactgg ccggtactct
    55021 gcgtgctgat tacgctgaca gcctgaccga aaatggtacc cacggttctg attccgtcga
    55081 atctgccgct cgcgaaatcg cttatttctt cggcgaaggc gaagtgtgcc cgcgcacccg
    55141 ttaataattt cgtaaatgcc gcgtgcaaac gtggcatccg tgcgccagaa tttgtacaat
    55201 gcagcgcccc cggacgagca gccgctcacc ggggcgtttc ttttttcaac cctccagggg
    55261 ccataacgtg taataacgag gccggaatag catatgtctg aacaattagt cacacctgaa
    55321 aacgtcacca ctaaagatgg aaaaatcaac ctgctggatc tcaaccgtca gcagatgcgg
    55381 gagtttttta aagatttagg tgaaaaaccc ttccgcgccg atcaggtgat gaagtggatg
    55441 tatcactatt gctgcgacaa ctttgatgag atgaccgaca tcaacaaagt gttgcgcggc
    55501 aaactgaaag aggtggcgga gatccgcgca ccggaagtgg ttgaagaaca gcgttcatct
    55561 gacggcacca ttaaatgggc aattgccgtt ggcgatcagc gcgtcgaaac ggtgtatatc
    55621 ccggaagacg accgtgccac gctctgcgtc tcttcgcagg tggggtgtgc gctggagtgt
    55681 aaattctgtt ccaccgccca gcagggcttt aaccgcaacc tgcgggtatc ggaaattatc
    55741 ggccaggtgt ggcgtgcggc gaaaattgtc ggcgcagcaa aagtcaccgg tcagcgccca
    55801 atcactaacg tcgtgatgat gggtatgggc gagccgctgc tcaacctgaa caacgtcgtt
    55861 ccggcgatgg aaatcatgct tgatgatttc ggttttggcc tgtcaaaacg tcgcgtcacg
    55921 ctttccactt ccggtgtggt tccggcgctg gataaactgg gcgatatgat cgacgttgcg
    55981 ctggctatct ccctgcacgc gccgaacgac gaaattcgtg acgaaattgt gccgatcaac
    56041 aaaaagtaca atatcgaaac gttccttgcg gcggtgcgcc gttatctgga gaaatccaac
    56101 gccaatcagg gccgagtcac tattgaatac gtgatgcttg accacgttaa cgacggcact
    56161 gaacacgcgc accaactggc ggaactgctg aaagatacgc cgtgtaagat caacctgatc
    56221 ccgtggaacc cgttcccggg cgcgccgtat ggacgcagtt cgaacagccg tatcgaccgt
    56281 ttctcaaaag tgctgatgag ctacggtttc accactattg tgcgtaaaac gcgtggtgat
    56341 gacattgatg ctgcctgtgg tcagttggcg ggcgatgtta tcgaccgtac gaaacgtacc
    56401 ctgcgtaaac gaatgcaggg tgaagccatc gacattaaag cggtctgata gccattacgc
    56461 cacggttact ttatgtaccg tggcgtaatc ttttatgaat gaagtattaa gttgcaattg
    56521 tctggtcaat attaacggtg cgtttttgct gactttaagg cagtatgtaa cgatgcaaca
    56581 gtaagttagc cttagcgaat gtgggctttg tcacgagcac acagacggtc ttatactgta
    56641 tgataacggt taacttaacg gatgtttcgc ggtgtgggtg ggcatgatgg ttcaccggca
    56701 tctcaattct catttaaacg tacctgcagc gaatgaatac tgaagccacg cacgaccaaa
    56761 atgaagcact tactaccggc gctcgcctgc gtaatgctcg cgaacaacta ggacttagtc
    56821 aacaggccgt tgccgagcga ctttgcctga aggtttccac ggtacgcgac attgaagaag
    56881 ataaggcacc cgccgatctt gcttcaacat tcctgcgcgg atatatccgc tcttatgcgc
    56941 gtctggtaca tattccagaa gaagaactgc tgccagggct ggaaaagcag gctccactac
    57001 gggctgcaaa agttgcgccg atgcagagtt tttccctcgg taaacgccgc aaaaaacgtg
    57061 acggctggct gatgaccttc acctggctgg tattgtttgt ggttattggc ctgagcggtg
    57121 cctggtggtg gcaagaccac aaagctcagc aggaagagat caccactatg gccgatcaat
    57181 cttctgcgga actgaataat aatcaaagcc agagtgtacc gttagatacg tcgacaacca
    57241 cagatcaggc tatggcgacc acgccgacat cacctgtgga taccaccgca accaacacgc
    57301 aaacacctgc cgtaactgcg ccagcaccag ctgttgatcc gcaacagaat gcggttgttc
    57361 caccttcgca ggcaaatgtt gataccgctg cgactccggc accagcggca acaacaacac
    57421 cagatggcgc tgcgccgttg ccaaccgatc aggctggcgt gaccacgccg gcggttgacc
    57481 cgaatgcgct ggtgatgaac tttactgccg attgctggct ggaagtcacc gatgctaccg
    57541 gtaaaaaact gtttagcggt atgcagcgta aagacggtaa tttgaactta accggccagg
    57601 caccgtacaa actgaaaatt ggtgcgccag ccgcagtaca gatccagtat caagggaaac
    57661 ctgtcgatct gagtcgtttt atcagaacta accaggttgc gcgtctgacc ctcaatgccg
    57721 aacaatcacc ggcgcagtaa cagacgggta acgcgggaga tttttcatgc ataaccaggc
    57781 tccaattcaa cgtagaaaat caacacgtat ttacgttggg aatgtgccga ttggcgatgg
    57841 tgctcccatc gccgtacagt ccatgaccaa tacgcgcacg acagacgtcg aagcaacggt
    57901 caatcaaatc aaggcgctgg aacgtgttgg cgctgatatc gtccgtgtct ccgtaccgac
    57961 gatggacgcg gcagaagcgt tcaaactcat caaacagcag gttaacgtgc cgctggtggc
    58021 tgacatccac ttcgactatc gcattgcgct gaaagtagcg gaatacggcg tcgattgtct
    58081 gcgtattaac cctggcaata tcggtaatga agagcgtatt cgcatggtgg ttgactgtgc
    58141 gcgcgataaa aacattccga tccgtattgg cgttaatgcc ggatcgttgg aaaaagatct
    58201 gcaagaaaag tatggcgaac cgacgccgca ggcgttgctg gaatctgcca tgcgtcatgt
    58261 tgatcatctc gatcgcctga acttcgatca gttcaaagtc agcgtgaaag cgtctgacgt
    58321 cttcctcgct gttgagtctt atcgtttgct ggcaaaacaa atcgatcagc cgctgcatct
    58381 ggggatcacc gaagccggtg gtgcgcgtag cggggcagta aaatccgcta ttggtttagg
    58441 tctgctgctg tctgaaggca tcggcgacac gctgcgcgta tcgctggcgg ccgatccggt
    58501 cgaagagatc aaagtcggtt tcgatatttt gaaatcgctg cgtatccgtt cgcgcgggat
    58561 caacttcatc gcctgcccga catgttcgcg tcaggaattt gatgttatcg gtacggttaa
    58621 cgcgctggag caacgcctgg aagatatcat cactccgatg gacgtttcga ttatcggctg
    58681 cgtggtgaat ggcccaggtg aggcgctggt ttctacactc ggcgtcactg gcggcaacaa
    58741 gaaaagcggc ctctatgaag atggcgtgcg caaagaccgt ctggacaaca acgatatgat
    58801 cgatcagctg gaagcgcgca ttcgtgcgaa agccagtcag ctggacgaag cgcgtcgaat
    58861 tgacgttcag caggttgaaa aataataacg tgatgggaag cgcctcgctt cccgtgtatg
    58921 attgaacccg catggctccc gaaacattga gggaagcgtt gagggttcat ttttatattc
    58981 agaaagagaa taaacgtggc aaaaaacatt caagccattc gcggcatgaa cgattacctg
    59041 cctggcgaaa cggccatctg gcagcgcatt gaaggcacac tgaaaaacgt gctcggcagc
    59101 tacggttaca gtgaaatccg cttgccgatt gtagagcaga ccccgctatt caaacgtgcg
    59161 attggtgaag tcaccgacgt ggttgaaaaa gagatgtaca cctttgagga tcgcaatggc
    59221 gacagcctga ctctgcgccc tgaagggacg gcgggctgtg tacgcgccgg catcgagcat
    59281 ggtcttctgt acaatcagga acagcgtctg tggtatatcg ggcctatgtt ccgtcacgag
    59341 cgtccgcaga aagggcgtta tcgtcagttc catcagttgg gctgcgaagt tttcggtctg
    59401 caaggtccgg atatcgacgc cgaactgatt atgctcactg cccgctggtg gcgcacgctg
    59461 ggtatttccg agcacgtaac tcttgagctg aactctatcg gttcgctgga agctcgtgcg
    59521 aattaccgcg atgcgctggt ggcattcctt gagcagcata aagaaaagct ggacgaagac
    59581 tgcaaacgcc gcatgtacac taacccgttg cgcgtactcg attcaaaaaa tccggaagtg
    59641 caggtgcttc tcaacgacgc tccggcatta ggcgattatc tggacgagga atctcgtgag
    59701 cactttgccg gtctgtgcaa actgcttgag agcgcgggta tcgcttacac cgtaaaccag
    59761 cgtctggtgc gcggtctgga ttactataac cgtaccgttt tcgagtgggt gactaacagc
    59821 ctcggttccc agggcaccgt gtgtgcaggt ggtcgttatg acggtctggt ggaacaactg
    59881 ggtggtcgtg caacaccggc agtcggtttt gcgatgggcc tcgaacgtct tgtattgtta
    59941 gttcaggccg ttaatccgga atttaaagcc gatcctgttg tcgatatata cctggtggct
    60001 tcaggtgctg acacacaatc tgcggctatg gcattagcag agcgtctgcg tgatgaatta
    60061 ccgggcgtga aactgatgac caaccacggc ggcggcaact ttaagaaaca gtttgcccgt
    60121 gctgataaat ggggtgcccg cgttgctgtg gtgctgggtg agtctgaagt ggctaacggc
    60181 acagcagtag tgaaggattt gcgctctggt gagcaaactg cagttgcgca ggatagcgta
    60241 gccgcgcatt tgcgcacgtt actgggttaa ggaaggagaa ggacagcgtg gaaatttacg
    60301 agaacgaaaa cgaccaggtt gaagcggtta aacgcttttt tgctgaaaat ggcaaagcac
    60361 tggctgttgg ggtgattttg ggcgttggcg cactgattgg ctggcgctac tggaatagcc
    60421 atcaggttga ttctgcacgc tccgcttctc ttgcctatca aaatgcggtt accgcagtga
    60481 gcgaaggcaa accggatagc atcccagcgg cggaaaaatt tgctgctgaa aataaaaata
    60541 cttatggtgc gctggcttct ttggaacttg cgcagcaatt tgttgacaaa aatgaactag
    60601 agaaagctgc cgcccagtta caacaggggc tggcagacac gagcgatgaa aatctcaaag
    60661 ccgtgataaa tctgcgtctt gctcgcgttc aggtacagct caagcaggct gatgccgcgc
    60721 taaaaaccct tgatgctatc aaaggtgaag ggtgggctgc cattgttgcc gacctgcgtg
    60781 gtgaagcatt gctgagcaaa ggtgataagc aaggtgcgcg tagtgcatgg gaagcaggcg
    60841 tgaaaagcga tgttaccccg gcactgagcg aaatgatgca gatgaaaatt aataatttgt
    60901 ccatctgaga gggacccgat gcaattgcgt aaattactgc tgccaggact gctttccgtt
    60961 acccttttaa gcggctgttc gctgtttaac agcgaagaag atgtggtaaa gatgtcccca
    61021 ttgccaaccg ttgaaaacca gtttacgccg actactgcgt ggagcacttc cgttggtagc
    61081 ggcattggca acttctattc caatcttcat ccggcactgg cggacaacgt tgtctatgca
    61141 gcggaccgcg ctggtttagt aaaagcgctg aatgcggatg acggcaaaga aatctggtct
    61201 gtcaacctgg ccgagaaaga tggctggttc tctaaagatc ctgcattact ttctggcggc
    61261 gtgaccgtgt ctggtgggca tgtctacatt ggcagcgaaa aggcgcaggt ttacgcgctg
    61321 aataccagcg atggtactgt ggcatggcaa actaaagtcg cgggtgaagc actttcgcgc
    61381 ccggtggtca gcgacggtct ggtgttaatc cacaccagta acggtcagtt acaagcgctg
    61441 aacgaagctg acggcgctgt caaatggaca gttaacctcg atatgccgtc gctctctttg
    61501 cgtggcgagt ctgcgccggc aacggctttt ggtgcggccg tcgtgggtgg cgataatggt
    61561 cgcgtcagcg cagtgctgat ggaacagggc cagatgattt ggcagcagcg tatttcccag
    61621 gcgaccggtt ctaccgaaat tgaccgtctg agcgatgttg acacgactcc cgtcgttgtt
    61681 aacggcgttg ttttcgcgct ggcctataat ggtaacctga cggcgcttga tctgcgcagc
    61741 ggtcagatta tgtggaaacg cgaactgggt tcggtgaatg atttcatcgt cgacggcaac
    61801 cgcatctatc tggtcgatca aaatgaccgg gtgatggcgt tgaccattga tggcggcgtt
    61861 acgctgtgga cacaaagcga tctgctgcat cgcctgctga cttctccggt gctgtataat
    61921 ggcaacctgg tggtaggtga cagtgaaggt tatctgcact ggattaacgt cgaagatggt
    61981 cgtttcgttg cccagcaaaa agttgatagt tccggtttcc agactgaacc ggttgctgct
    62041 gacggcaaac tgctgatcca ggcaaaagac ggaaccgtgt actctattac acgttaatcg
    62101 tctctgtcgt tcactttgaa aacggctcct ggacaggggc cgttttcctg tttttaacaa
    62161 cgacacgaat atagcgtgcg ttgtctgatg atttataaaa atgaggcttt aaacatggta
    62221 cctgtggtcg cgcttgtcgg gcgccctaac gtaggaaaat ccacgttatt taaccgtcta
    62281 actcgcaccc gagatgcgct ggttgcggat ttcccgggtc tgactcgtga ccgtaagtac
    62341 ggtcgtgcgg aaattgaagg ccgcgagttt atctgcattg ataccggcgg gattgatggc
    62401 accgaagacg gtgtagagac acgtatggcg gaacagtcgc tgctggcgat tgaagaagcg
    62461 gacgttgtac tgtttatggt ggatgcgcgc gcgggcctga tgccggcgga tgaagcgatt
    62521 gctaaacatc tgcgctcccg tgaaaaaccg accttcctgg tggcaaacaa aactgacggt
    62581 ctggatcccg atcaggcagt ggttgatttc tacgcgcttg gtttaggtga aatctacccg
    62641 atcgccgcgt ctcacggtcg tggcgtatta agtctgctgg agcacgtgct gctgccgtgg
    62701 atggaagatc tcgcaccgca agaagaagtc gacgaagacg ccgaatactg ggcgcaattt
    62761 gaagcggaag agaacggcga agaagaagag gcagacgact tcgacccgca aagtctgccg
    62821 atcaaactgg cgattgtggg tcgtccgaac gtaggtaagt ctacactcac taaccgtatt
    62881 cttggtgaag agcgcgttgt tgtttacgac atgcctggca cgacgcgtga cagtatctac
    62941 attccaatgg aacgcgacgg acgtgagtat gtgctcattg acaccgctgg tgtacgtaaa
    63001 cgcggcaaaa tcaccgatgc ggtagagaaa ttctcggtaa tcaaaacgtt gcaggccatt
    63061 gaagatgcca acgtggtgat gttggtgatt gatgcgcgtg aaggtatttc cgatcaggat
    63121 ctctccttgc tgggctttat tctcaatagt gggcgctcac ttgtgattgt agtgaacaag
    63181 tgggacggcc tgagccagga agtgaaagag caggtgaaag agacgctgga cttccgtctg
    63241 ggctttatcg attttgctcg tgtgcacttt atctctgcct tgcacggcag tggtgttggt
    63301 aacttgtttg aatcagtacg tgaagcgtat gacagctcca cccgtcgtgt ggggacctct
    63361 atgctgacgc gcatcatgac gatggctgtt gaagatcacc aaccaccgct ggtacgcggt
    63421 cgtcgtgtga agctgaaata cgcccacgcc gggggttata acccgccgat tgtggtgatt
    63481 cacggcaatc aggtgaaaga cctgcctgat tcctacaagc gttacctgat gaactacttc
    63541 cgcaaatcgc tggacgtaat gggatcgccg attcgtattc agttcaaaga aggggaaaac
    63601 ccgtatgcga acaagcgtaa caccctgacg ccaacccaga tgcgtaaacg taagcgtctg
    63661 atgaagcata tcaagaaaag taagtaatca cttatctggt ttccctttcc acacggagag
    63721 ggaagccgaa taacacgcga ggagtattta tggcactcaa ctgtccaacc tgcaaaaccc
    63781 ctctcgaacg caatggcgag actgctcact gtgcaacttg cgacaaagac tttaccgtcc
    63841 aggcactttg ccctgagtgt catcaggcgt tacaagtgtt aaaagcttgt ggtgctgtgg
    63901 attatttctg ccaacatggg cacggtttaa tatctaaaaa a
//
LOCUS       DAZKJR010000010        59632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKJR010000010 DAZKJR010000000
VERSION     DAZKJR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.010"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..837)
                     /gene="rfbA"
                     /locus_tag="ACHOWS_000644"
     CDS             complement(<1..837)
                     /gene="rfbA"
                     /locus_tag="ACHOWS_000644"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0046282.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFHQLLGDGGQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGGLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKN"
     gene            complement(895..1794)
                     /gene="rfbD"
                     /locus_tag="ACHOWS_000645"
     CDS             complement(895..1794)
                     /gene="rfbD"
                     /locus_tag="ACHOWS_000645"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0046283.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEQCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVATTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            complement(1794..2879)
                     /gene="rfbB"
                     /locus_tag="ACHOWS_000646"
     CDS             complement(1794..2879)
                     /gene="rfbB"
                     /locus_tag="ACHOWS_000646"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0046284.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            complement(3251..4144)
                     /gene="galF"
                     /locus_tag="ACHOWS_000647"
     CDS             complement(3251..4144)
                     /gene="galF"
                     /locus_tag="ACHOWS_000647"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFQ0046285.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(4387..5382)
                     /locus_tag="ACHOWS_000648"
     CDS             complement(4387..5382)
                     /locus_tag="ACHOWS_000648"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFQ0046286.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLTAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(5540..6934)
                     /gene="wcaM"
                     /locus_tag="ACHOWS_000649"
     CDS             complement(5540..6934)
                     /gene="wcaM"
                     /locus_tag="ACHOWS_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFQ0046287.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            complement(6945..8165)
                     /gene="wcaL"
                     /locus_tag="ACHOWS_000650"
     CDS             complement(6945..8165)
                     /gene="wcaL"
                     /locus_tag="ACHOWS_000650"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFQ0046288.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLCGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(8162..9442)
                     /gene="wcaK"
                     /locus_tag="ACHOWS_000651"
     CDS             complement(8162..9442)
                     /gene="wcaK"
                     /locus_tag="ACHOWS_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFQ0046289.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(10103..11581)
                     /gene="wzxC"
                     /locus_tag="ACHOWS_000652"
     CDS             complement(10103..11581)
                     /gene="wzxC"
                     /locus_tag="ACHOWS_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFQ0046290.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDTI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLIVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(11583..12977)
                     /gene="wcaJ"
                     /locus_tag="ACHOWS_000653"
     CDS             complement(11583..12977)
                     /gene="wcaJ"
                     /locus_tag="ACHOWS_000653"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFQ0046291.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(13032..14402)
                     /gene="cpsG"
                     /locus_tag="ACHOWS_000654"
                     /gene_synonym="manB"
     CDS             complement(13032..14402)
                     /gene="cpsG"
                     /locus_tag="ACHOWS_000654"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFQ0046292.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     NNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(14595..16031)
                     /gene="cpsB"
                     /locus_tag="ACHOWS_000655"
     CDS             complement(14595..16031)
                     /gene="cpsB"
                     /locus_tag="ACHOWS_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFQ0046293.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAIDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(16034..17257)
                     /gene="wcaI"
                     /locus_tag="ACHOWS_000656"
     CDS             complement(16034..17257)
                     /gene="wcaI"
                     /locus_tag="ACHOWS_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFQ0046294.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWQPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVANADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(17254..17733)
                     /gene="gmm"
                     /locus_tag="ACHOWS_000657"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(17254..17733)
                     /gene="gmm"
                     /locus_tag="ACHOWS_000657"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFQ0046295.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVVEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(17736..18701)
                     /gene="fcl"
                     /locus_tag="ACHOWS_000658"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(17736..18701)
                     /gene="fcl"
                     /locus_tag="ACHOWS_000658"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFQ0046296.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(18704..19825)
                     /gene="gmd"
                     /locus_tag="ACHOWS_000659"
     CDS             complement(18704..19825)
                     /gene="gmd"
                     /locus_tag="ACHOWS_000659"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFQ0046297.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYDMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(19852..20400)
                     /gene="wcaF"
                     /locus_tag="ACHOWS_000660"
     CDS             complement(19852..20400)
                     /gene="wcaF"
                     /locus_tag="ACHOWS_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFQ0046298.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(20416..21162)
                     /gene="wcaE"
                     /locus_tag="ACHOWS_000661"
     CDS             complement(20416..21162)
                     /gene="wcaE"
                     /locus_tag="ACHOWS_000661"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFQ0046299.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAHGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(21173..22390)
                     /gene="wcaD"
                     /locus_tag="ACHOWS_000662"
     CDS             complement(21173..22390)
                     /gene="wcaD"
                     /locus_tag="ACHOWS_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFQ0046300.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(22365..23582)
                     /gene="wcaC"
                     /locus_tag="ACHOWS_000663"
     CDS             complement(22365..23582)
                     /gene="wcaC"
                     /locus_tag="ACHOWS_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFQ0046301.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQRVAGKRQLFSEMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQSSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(23579..24067)
                     /gene="wcaB"
                     /locus_tag="ACHOWS_000664"
     CDS             complement(23579..24067)
                     /gene="wcaB"
                     /locus_tag="ACHOWS_000664"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFQ0046302.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(24070..24909)
                     /gene="wcaA"
                     /locus_tag="ACHOWS_000665"
     CDS             complement(24070..24909)
                     /gene="wcaA"
                     /locus_tag="ACHOWS_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFQ0046303.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(25087..27249)
                     /gene="wzc"
                     /locus_tag="ACHOWS_000666"
     CDS             complement(25087..27249)
                     /gene="wzc"
                     /locus_tag="ACHOWS_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFQ0046304.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGEITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(27252..27695)
                     /gene="wzb"
                     /locus_tag="ACHOWS_000667"
     CDS             complement(27252..27695)
                     /gene="wzb"
                     /locus_tag="ACHOWS_000667"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFQ0046305.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFVAVYTLLERSARQWAQALNAEQV"
     gene            complement(27701..28840)
                     /gene="wza"
                     /locus_tag="ACHOWS_000668"
     CDS             complement(27701..28840)
                     /gene="wza"
                     /locus_tag="ACHOWS_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFQ0046306.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            29149..29298
                     /locus_tag="ACHOWS_000669"
     CDS             29149..29298
                     /locus_tag="ACHOWS_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046307.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            29499..31082
                     /gene="yegH"
                     /locus_tag="ACHOWS_000670"
     CDS             29499..31082
                     /gene="yegH"
                     /locus_tag="ACHOWS_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFQ0046308.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            31157..31495
                     /locus_tag="ACHOWS_000671"
     CDS             31157..31495
                     /locus_tag="ACHOWS_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0046309.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSNIDLAKRRYK
                     ELVQERKNEN"
     gene            31485..31775
                     /locus_tag="ACHOWS_000672"
     CDS             31485..31775
                     /locus_tag="ACHOWS_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0046310.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(31828..33681)
                     /gene="asmA"
                     /locus_tag="ACHOWS_000673"
     CDS             complement(31828..33681)
                     /gene="asmA"
                     /locus_tag="ACHOWS_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFQ0046311.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLKETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVIGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(33703..34284)
                     /gene="dcd"
                     /locus_tag="ACHOWS_000674"
     CDS             complement(33703..34284)
                     /gene="dcd"
                     /locus_tag="ACHOWS_000674"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFQ0046312.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(34376..35017)
                     /gene="udk"
                     /locus_tag="ACHOWS_000675"
     CDS             complement(34376..35017)
                     /gene="udk"
                     /locus_tag="ACHOWS_000675"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0046313.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            35335..38652
                     /gene="dgcE"
                     /locus_tag="ACHOWS_000676"
     CDS             35335..38652
                     /gene="dgcE"
                     /locus_tag="ACHOWS_000676"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFQ0046314.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(38761..39609)
                     /gene="alkA"
                     /locus_tag="ACHOWS_000677"
     CDS             complement(38761..39609)
                     /gene="alkA"
                     /locus_tag="ACHOWS_000677"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFQ0046315.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEC
                     RGVVTAIPDIASHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPLRSYALLHIWYTEG
                     WQPDEA"
     gene            39743..41095
                     /gene="yegD"
                     /locus_tag="ACHOWS_000678"
     CDS             39743..41095
                     /gene="yegD"
                     /locus_tag="ACHOWS_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFQ0046316.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(41108..43047)
                     /gene="yegI"
                     /locus_tag="ACHOWS_000679"
                     /pseudo
     CDS             complement(41108..43047)
                     /gene="yegI"
                     /locus_tag="ACHOWS_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(43044..43805)
                     /gene="pphC"
                     /locus_tag="ACHOWS_000680"
     CDS             complement(43044..43805)
                     /gene="pphC"
                     /locus_tag="ACHOWS_000680"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFQ0046317.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWLP"
     gene            complement(43802..44461)
                     /gene="yegL"
                     /locus_tag="ACHOWS_000681"
     CDS             complement(43802..44461)
                     /gene="yegL"
                     /locus_tag="ACHOWS_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFQ0046318.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(44682..44741)
                     /gene="ibsA"
                     /locus_tag="ACHOWS_000682"
     CDS             complement(44682..44741)
                     /gene="ibsA"
                     /locus_tag="ACHOWS_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFQ0046319.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(45014..45070)
                     /locus_tag="ACHOWS_000683"
     CDS             complement(45014..45070)
                     /locus_tag="ACHOWS_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFQ0046320.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(45342..45398)
                     /locus_tag="ACHOWS_000684"
     CDS             complement(45342..45398)
                     /locus_tag="ACHOWS_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFQ0046321.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(45670..45726)
                     /locus_tag="ACHOWS_000685"
     CDS             complement(45670..45726)
                     /locus_tag="ACHOWS_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFQ0046322.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            46005..47252
                     /gene="mdtA"
                     /locus_tag="ACHOWS_000686"
     CDS             46005..47252
                     /gene="mdtA"
                     /locus_tag="ACHOWS_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFQ0046323.1"
                     /translation="MKSSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            47252..50374
                     /gene="mdtB"
                     /locus_tag="ACHOWS_000687"
     CDS             47252..50374
                     /gene="mdtB"
                     /locus_tag="ACHOWS_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFQ0046324.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            50375..53452
                     /gene="mdtC"
                     /locus_tag="ACHOWS_000688"
     CDS             50375..53452
                     /gene="mdtC"
                     /locus_tag="ACHOWS_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFQ0046325.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRIGLNPQALFNQGVSLDDVRSAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGALRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            53453..54868
                     /gene="mdtD"
                     /locus_tag="ACHOWS_000689"
     CDS             53453..54868
                     /gene="mdtD"
                     /locus_tag="ACHOWS_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0046326.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRCFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNCALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            54865..56268
                     /gene="baeS"
                     /locus_tag="ACHOWS_000690"
     CDS             54865..56268
                     /gene="baeS"
                     /locus_tag="ACHOWS_000690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFQ0046327.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            56265..56987
                     /gene="baeR"
                     /locus_tag="ACHOWS_000691"
     CDS             56265..56987
                     /gene="baeR"
                     /locus_tag="ACHOWS_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFQ0046328.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLMIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLEALDEE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            57178..57510
                     /gene="yegP"
                     /locus_tag="ACHOWS_000692"
     CDS             57178..57510
                     /gene="yegP"
                     /locus_tag="ACHOWS_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFQ0046329.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            57658..59019
                     /gene="yegQ"
                     /locus_tag="ACHOWS_000693"
     CDS             57658..59019
                     /gene="yegQ"
                     /locus_tag="ACHOWS_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFQ0046330.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTTFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(59361..>59632)
                     /gene="yegR"
                     /locus_tag="ACHOWS_000694"
     CDS             complement(59361..>59632)
                     /gene="yegR"
                     /locus_tag="ACHOWS_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFQ0046331.1"
                     /translation="GCAVHNDETSIGKFGLAYKSNIQRKLDNQYYTEAEASLARGRIS
                     GAENIVKNDAVHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT        14014 a        15933 c        15121 g        14564 t
ORIGIN      
        1 attttttttc agcggttcag ctaatacttt cacctgctca gcatcaataa accctttacg
       61 gtaagcaatt tcttccggac aggaaacttt taacccctga cgctcttcaa ttgttgcaat
      121 gaagttgctt gcttcaataa gactttgatg tgtacccgtg tccagccatg cataaccacg
      181 tcccatcatg gcaacggata accccccctg ttccatataa atacggttaa tatcggtaat
      241 ttccagttca ccacgggcag aaggcttaag gtttttcgcc atttcgacaa cgtcgttatc
      301 atagaaataa agcccggtta ccgcataatt actttttggt tgtaacggtt tttcttccag
      361 gctaattgcc gtaccttttt tatcaaactc aacgacaccg tagcgttcag gatcattaac
      421 atgataggca aatactgttg caccactttc tttgttaaca gcgacatcca ttaacttcgg
      481 cagatcatga ccgtaaaaga tattatcacc gagaaccaga gcacaatcat caccaccgat
      541 aaactcttca ccgataataa acgcctgcgc aagcccatct ggagtcggtt gcactttgta
      601 ctgaagattt agcccccact ggcccccgtc acccagcagt tggtgaaaac gaggagtatc
      661 ctgtggtgta ctgataatca gaatatcgcg aatacccgct aacatcagtg tagaaagcgg
      721 gtaatagatc atcggtttat cataaatagg taacagctgt ttactgacag ccatagtcac
      781 aggataaaga cgagtaccag agccacccgc taaaataata cctttacgcg ttttcatttc
      841 atcattcctt ttaattcatc ttgctccatc atcacgaaca agatgcaaaa actattaaat
      901 tactgtagtc gtaaataatt cgttgagcat tcgtttcacg ccaacctgcc agtcaggcaa
      961 aacaagcgca aaattctgct gaaatttttc tgtattaagg cgagagttat gtggacgacg
     1021 agctggtgta ggataggctg ttgttgctac tgcgttgagc ttgttgagtg caaggggtat
     1081 gcctgctttg cgcgcctctt caaaaaccag cgcagcataa tcgtgccagg ttgtggtacc
     1141 actggctacc agatggtaca aacctgcgac ttccggttta ttcagtgcca cacgaatggc
     1201 atgtgccgta caatcagcca gcaactctgc gccagttggc gcaccaaact gatcattaat
     1261 aacggctaat tcttcacgct cttttgccag acgtaacatt gtcttggcaa aattgtttcc
     1321 tttacctgca tatacccagc tggtacggaa aataaggtgc ttcgcacact gctcttgtaa
     1381 tgctttttct ccagctaact tggtttcacc gtaaacattt agcggtgcgg ttgcatccgt
     1441 ctccagccat ggtatttcac cggttcccgg aaatacgtag tcagttgagt aatgaattac
     1501 ccaggcccca acttcattag ccgcttttgc aattgattca acgctggtcg cattgagtaa
     1561 ttgtgcaaat tcgggttctg actcagcctt atctactgcg gtatgagccg cagcattaac
     1621 aataacatca gggcgaattt ttttgacggt ttcagccaca ccttcaggat tactaaaatc
     1681 accacaataa tcagtggagt gaacatcaag agcaatcaaa ttacccaaag gtgccagagc
     1741 acgctgtagt tcccaaccta cctgccctgt tttgccgaaa aggagaatat tcattactgg
     1801 cggccctcat agttctgttc aatccacgat tgataggcac cacttttcac attatcaacc
     1861 cattttgtat tggacaggta ccattccact gtcttccgaa tcccgctctc aaacgtttcc
     1921 tgtggtttcc atcccaattc gcgaccaatc ttctcagcat caatggcata acggcgatcg
     1981 tgtcccggac gatcggcaac ataagtaatt tgctcgcggt aagatttctc tttcggtaca
     2041 atctcatcca acaaatcaca aatagtgagc actacatcga tgtttttctt ttcgttgtgt
     2101 ccaccaatgt tataagtttc acccgcttta ccttcggtta cgacggtata taacgcacgt
     2161 gcatgatctt caacatacag ccagtcgcga atttgatccc ctttgccata aataggtaat
     2221 gccttacctt ccagagcatt aagaattact agtggaatca atttttccgg aaagtgataa
     2281 ggaccgtagt tattcgaaca attagtcaca atggtcggta aaccataggt acgtttccac
     2341 gcacggacta aatgatcgct ggatgctttt gatgcggaat aaggactact aggcgcgtaa
     2401 gctgtcgtct cagtaaagag gggtaattgt tctttattat ttacttcgtc aggatgaggc
     2461 aaatcaccat agacttcgtc agtagaaata tgatgaaaac ggaagctatt tttcttgtcg
     2521 ccatcaagag cagaccaata attgcgagcg gcttccaaaa ggacataagt accaacaata
     2581 ttggtttcaa taaatgccgc agggcctgta attgaacgat ccacatggct ttcagcagcc
     2641 aggtgcatca ctgcatccgg ctgatgctga gcaaaaatcc gcgccattgc agcagcatcg
     2701 caaatatccg catgttcaaa aatatagcgt tcagaatcag aaacatcagc aagtgattcc
     2761 aggtttccgg cgtacgttaa tttatcgaca ttaacaacac tatcctgcgt attatttata
     2821 atgtgacgaa ctacagcaga accaataaat cctgcgccac cagtaacaag tatcttcact
     2881 cttttattcc atattacttc agagcatgct gtgaaataag cggctcttag tttgattaat
     2941 agaggtatta atgcacgcta ccgcccctgg ctttacagct accagagcac tgcatgcatg
     3001 cctacgatgt gacgagcgtt acccactcgc gcttaactcg aaaaattcaa acgctaattg
     3061 tcttaccaat ccgctctgga aacaaggaaa atcctggaaa actttgacga aaatcctatt
     3121 gctaactctt tgttattctg attgtttata taaaacaacg gcaggaatat tcgcaacaaa
     3181 ttactttcgc cacgaatctt cactgccgtt ataattttct tatcaaccgt tacatccggt
     3241 cagattttca ttattcgctt aacagcttct caataccttt acggaacttc gccccttctt
     3301 tcaggttgcg cagtccatac ttcacaaacg cctgcatata gcccattttt ttaccacagt
     3361 cgtagctgtc accagtcatc agcatggcat caacggactg ttttttcgcc agttcggcaa
     3421 tagcatcagt cagctgaata cgcccccatg caccaggctg agtgcgttcc agttccggcc
     3481 aaatatcggc agaaagcaca tagcgaccaa cggccatgat gtctgagtcc agcgtctgcg
     3541 gctgatccgg tttttcgatg aattcaacaa tgcggctgac tttgccttca cgatccagcg
     3601 gttctttggt ctggatgacg gagtattcag agaggtcacc cggcatacgt tttgccagca
     3661 cctggctgcg gcccgtttcg ttgaagcgcg caatcatagc ggcaaggttg taacgcagcg
     3721 ggtcggcgct ggcatcatcg ataacaacgt ccggcagcac cacgacaaat gggttgtcac
     3781 caatggcggg gcgtgcacac aaaatggagt gacctaaacc taaaggttcg ccctgacgca
     3841 cgttcataat agtcacgccc ggcgggcaga tagattgcac ttccgccagt agttgacgct
     3901 tcacgcgctg ctcaaggaga gattctaatt cataagaggt gtcgaagtgg ttttcgaccg
     3961 cgttcttgga cgcgtgagtt accaggagga tttctttgat ccctgcagcc acaatctcgt
     4021 caacaatgta ctgaatcatt ggcttgtcga cgatcggtag catctctttg ggaatcgcct
     4081 tagtggcagg caacatatgc atcccaagac ctgctaccgg tataactgct tttaaattcg
     4141 tcattctttt cctacctcta agtggctgat agtgcgtaaa ttattgccat aggttagcca
     4201 aacgttatgg ctatatacca aagcttagtt ccgcataact ttgcttaaac cttagaataa
     4261 cactacacac catcagcatc tgggttactc ggattactcg gaattccgca tactgataat
     4321 ttaatcagta cctctttccg aataatcgta gtccaacctg gtcctttttt ctctgactcg
     4381 tctgcattac tcagaaacaa acgttatgtc gtcttttttg gcatgaacga attcatactg
     4441 cagtgttcga tccagacctt gcgacagcgt atacggtgcc acaaaacctg aagaatgcac
     4501 tttcgttgcg tcaaactgtg ttgttgcgca gaattttttc acgcgcacag agctgacagc
     4561 gtattttttg cccgtaattt tgctcaggat atcaaagcaa tatccaccca acattcctag
     4621 tgggtaaggc aagtgcatag aagggatctt tttgttcaga ctttgttcaa cttcagcaac
     4681 caactggttc atgttcagat ctggcttatc aacgtaatta taaacctcat aacctgcggc
     4741 aacattcttc agtttgtact tgataaactc aacaatgttt ccaacataag ccatggactt
     4801 atagttagtc cctgcgccca ccatcataaa cttgccgcca gcgatctgtt tcagcaagtt
     4861 atagacgtta ccgcggttgc gttcaccgaa gataacggta ggacggatga tggttaatga
     4921 acgttctgtt ggtgctttgt tataccattc acgcagcact tcctctgcct gccacttact
     4981 tttgccgtag tggttgaaag gatcgtgtgg atggttttcg tcagggttgt gtttgttcaa
     5041 accataaaca gcaacggaac tggtaaagat gatattttta acgccatttt tttccatggc
     5101 cgtcagcaca ttgcgggtac cctgaacgtt gacatcataa tagagagaag tagggctgac
     5161 gtcatcgcgg tgttccgctg ccagtagcac aacagtgtca aaaccagcta acgcctggtc
     5221 gagtgcctgt tgatcacgaa catcgccaat ctgtgtgatt tctggataaa agtggctctg
     5281 ctgtttgtcc aggttcttga tattaaagtc agcaattgcc gtttcaagta gtcgggttcc
     5341 tacgaatccg gaagctccta tgagcaaaac gttatcgttc ataaatcact ttagtctggt
     5401 tgttacgtaa gaaacacaag ataaagatga gtaccttccc tgagtagtca atgctgccca
     5461 gccccagctt taacagttag tgtgaggatt ataatctttt agaacattat atccagtaag
     5521 tttatgaatg gtcgcaaatc tactccctcc gttctggcaa tctaaagtta atcttttcca
     5581 cattaacaat atggtgatta accctgtcga tatcgacgga gctttgccct ctttcattca
     5641 tcgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag acgccgcgaa
     5701 cgtctttgcg catgtcgaag ttcatgctca atgcgggtcc aacagaggat tcctgcatca
     5761 ctttgatatt tcgcataaaa agatgttgcg gtttattgtg aagctccagc gaggcacgct
     5821 tcatctcaat gttagtcagc gccacaaagg agacagcatt cccggcggag atttggatgc
     5881 cgcgcaattt ataagcaagg tgagtgttat ccagttgaat attattcact cggaaatttt
     5941 gcggtatcga gagatatttg cctttaatta ccccatagcc aattaacatc ccggcgctat
     6001 taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg accgtggcgt
     6061 tatcaatacc tgctttctta ctgaaatccg gcgtgatatt gcgggctttg atattacgaa
     6121 taacaaaatg tttaccattt tcaacatgta tcaactgtcg acaatctgat cccgtgatat
     6181 tcgccacgac aaagtttttc actgcctggt cttccggata gttgttatcg taagtgcttc
     6241 ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg atgcgctcaa
     6301 taacgtggtc ggatatcaaa atatcgcggt cgttaatcgc cacattccat tcaatggcgt
     6361 cgccctgtaa gtcgctgaac ttacagttgg tgatgttggc accgataatc tggttatgaa
     6421 atccctggcg taaaatggcg taattagcgt ggctgacggt caggttatcg atggtcaagt
     6481 tgcgcatgac gcgtttattt ttgccgccga tataaatctg cgttaccggg ccaaaaccgc
     6541 tcatcgccaa ccctttgatg atgcagtcag aaccgcgcac atccagggtg atgttatgca
     6601 tactgccgcc ctcctcccct gtcacctggc tgccgtcctg taagacaaat cgccctctgc
     6661 cgttgccgcg caggcttcca aggatgtgta aggtttttcc aggaggaatg aagatgccgg
     6721 tgttgatatt gtcacaaacc aatccggcag gcacgacgac cgtttgccct tcactgaagg
     6781 cttgtttaaa tgaggcgatc cagtcgtgcg gattgtaatc gttgatatta acgctttgtc
     6841 gggctggaag tgcgcgggcg aagggggtat ggaggaaggc aagcgccgag cttgccgtca
     6901 ggaaggtgcg tcgggagagt tttttaaatg gcatacgttc tcctctataa agcctgcagc
     6961 aagctggcga gttctcgatt gatcacctgc tggttaaaat cgtgttcgac tttttcgcga
     7021 gcacgtttga taaccggagc cagttcgtcg gtgtccagtt ggctaaacgc cgccagtcgt
     7081 tgcgccagtg cgcgagcatc gttctcaggc accagccagc cggatttatc ggcctccacc
     7141 agttccggta ttccgctatg cagagtagaa accaccggaa tgccgaccgc catcgcttcc
     7201 attagtgcca ccggaatacc ttccatatcg ccgtccgcac ctgtaaccga tggcaacagg
     7261 aagacatccg cgtcgtcgag catcgccttc acttcatggc tcggtttaaa gcccggcatc
     7321 tctatcacat cttccagttg atattgttcg atgagggtgc gcaggcgtcg ttcccacggg
     7381 ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg acggcaggct
     7441 tcgatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacggaaat gatttccagc
     7501 ggcgttgcag gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac gcccatacgc
     7561 gatacggcga ttttttccct cgggcagccc attttttgca gccttccggc ccacagatcg
     7621 cttatcggta acatcaggtc gccacggcgg aacagttgct gatattcggg agtgtaatgg
     7681 ttgagcacct cccgactgga gatatcaata ccgtggaaga tagtggcaat tttgccgcga
     7741 atgacaccca gttcgcggag ttttgctgcg gttacccctg ccggaccaaa gtgagcgata
     7801 aagacatcgg catgaaacgg tgttgcgacc tgaccgcaaa tagcagacaa aatcaggttc
     7861 cgcgactcgg cgccatagcg tttgaggttg agcgcctgcc aggtattttt acgatgaatg
     7921 ccgcataagg tctggctggc gcggtggcgc agtttcgcca ctttgcccgt aggttcgtcc
     7981 tgtaaccagc gggttctggc agcaaggttg tatttcgtcc atgccgcgtg ggtgttttgt
     8041 gtgtcgcctt tttgcagcgc gataatctcc acctcaaatc ccatatcaat aaacgcagtg
     8101 atttggttaa gaacgaaggt ttccgacgac aacggaaatt tcagtaaaaa gaagccgacc
     8161 ttcatttcac ctccccgatg cgttcaagca cagattgcac catctgcatt cccgtctgac
     8221 gttcgcgact gacggcttcg ttaagtcgcg tgttcagtgc cggaagctgg cctaaggtat
     8281 ccgcaaccat cgcttgcagg ctgccgtcta ataaatgacg gatatcaatc gccatttctg
     8341 gtagccccag ttgttgcatg atcccggcgg atttatgttc gtagttgatg gcaatcgccg
     8401 gagtggcaaa gttcatcgag ataatagcag agtgcaggcg cgtaccgacg gtaagttcac
     8461 aggcccccag aattttgccc atttccagat cgttgagttc atccatcact acgtgataac
     8521 gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg tctttgttat
     8581 aactgtcgat gcccgtacag gtggagagcg caatcacctg atacccttca tcgagaatgc
     8641 gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg agacgtttgt
     8701 cgaacggtgc cagttcgcgc agggtaattg ccaccgtttt ctgttgtgcg gcaacatcca
     8761 gccagtgttg aacagcatag ctggcggtga agtcttcaat gtggtgatcg accagccacg
     8821 cggtatcgac gccatgttcc acttttgcag tggtgatatt gctgcgtttc atcaaatcaa
     8881 ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag ttcgccagtt
     8941 ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac agcggctttt
     9001 tcgccatgaa agtgcaaagc gcatgttcaa actgcggcac gccgtagaga tcgacaaaaa
     9061 acgatccgcc gacctgaata atggcgtcgt aacctgacag cagtcgcacg aagtcagtga
     9121 atccctgggc gatggcgata ttacgcagct tgccagtgtc ggtgacgcgt gagagcaata
     9181 cctggtgctg gtagcggcga cggaggactt ttttaacacg cccgacaacg cccgccgcgc
     9241 tgttgtgttg tttcatttgc aggaacagcg gatcgcccat taccgggcgg ttgagcagcc
     9301 aggaagaact gaccggatag cggctcatca cgtccacttc ggcatgtgga ttgaggatat
     9361 tgatggcatc aagtaagcca cgcaggatgg cgctatcgcc acgattgccg caagtgtggt
     9421 tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg aggctgtgtt
     9481 tgttgtgttc gttgccgttt tttgcctgat gcgacgctaa cgcgtcttat caggcctaca
     9541 agatccgagc acagaaccgt aggacggata aggcgttcac gccgcatccg gcattcagtg
     9601 cctgatgcga cgctgacgcg tcttatcagg cctacaagat ccaagcccag aaccgtagga
     9661 cggataaggc gttcacgccg catccggcat tcagtacctg atgcgacgct gacgcctctt
     9721 atcaggccta caagatccaa gcccagaacc gtaggacgga taaggcgttc acgccgcttc
     9781 cggcattcag tgcctgatgc gacgctgacg cgtcttatca ggcctacaag acccgagcac
     9841 agagccgtag gacggataag gcgttttacg ccgcatccgg cattcagtgc ctgatgcgac
     9901 gctgacgcgt cttatcaggc ctacaagatc cgagcacaga accgtaggcc ggataaggcg
     9961 ttcacgccgc atccggcatt cagtgcctga tgcgacgctg gcgcgtctta tcaggcctac
    10021 aagatccaag cccagccccc caggacggat aaggcgtttt acgccgcatc cggcaaccgt
    10081 tgccggaacc gaaaacaacc attcaccccg cccgtaaaag cattttcatt ttttcgctgc
    10141 gacaaaactg acgcttcact tccaccacca gcggatggcg cgacagcaca atcatcacca
    10201 caaacgccag cacgcccgtg gctatttgca ccgccagcag catccccagc gccagttgcc
    10261 ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcagcgag agataaaacg
    10321 gcagccacaa actcaggatg tactggcgat aactggaacc gagcaccggt ttaatcatca
    10381 cgaaatagct cagaatggtg ttgataatct gcaccagcag gaagccaagc gtgacgccga
    10441 ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaag gttttgaata
    10501 cgttgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa ccaatcgggt
    10561 tccccactga gcgcagcagc cccaccacac acagcaattg cagcaccgga ataatgctgt
    10621 tccacttctc accaaatacc agcggtacaa agttattcga caccactatc agccccagca
    10681 gcgccggaaa gttgataatc cccaccaccg acagcagctt gtagaagttg acgcgcagct
    10741 tttcggtgtc gtcctgaatt ttggcaaatg ccggaaacaa cacgcgcgtg atgatggggt
    10801 tcagtttcat cggcggcaca acggccacgt tgtacgccag gttgtatccc cctgccacgc
    10861 ccgcgccgag aatacgcgcc agcacgagtg ttgaaaggtt ggtattgaga tagttgatga
    10921 tgctgtccgc cgtcagccag gcaccaaaac gtaagtttgg tgccaccgac gccagcgaga
    10981 aatgcagacc ggggcgataa attttgcggc caaagtagcc aaacagcagc gttcttaccg
    11041 cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa tgggcgctaa
    11101 ccaccgtaaa agtgaagccc gccagcaccg cactggtttc gatcatgccg attttgttga
    11161 actccagctc cttctgcatc aacgcgcgga actgctgccc gtgggggatc accacaaacg
    11221 ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt attcagcacg tcgccaatgg
    11281 tatcactcaa caaaaacacc gccacgcaca ccacgatccc cagcccgacg ttcagccagt
    11341 acagcgtggt gagttcaagg tggctgattt cttttcgctg aataatcgag ttagcgatgc
    11401 cgaagtcaga aagcgtatct gccagcgcga tgatcaccag cgacacggta agcaaaccga
    11461 actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg aggccgatga
    11521 tgatcaccgt ggcaatcgcc gaccacttcg cgccgctgat ggttttttca cgtaagttca
    11581 tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac gattttgata
    11641 tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg tttttccatt
    11701 ttctccagtg tgtcggtttc gccgcgccag ccgttaatct gcgcccagcc ggtaatgccc
    11761 ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg ttcgttatgc
    11821 gccaccgcgt gcggacgtgg cccgacaatc gacatcccgc cagtcagcac attgataaac
    11881 tgcggcaatt catccagcga ggtgcggcgc agaaagttcc ccactttggt gacgcgcgga
    11941 tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat ggaacggaac
    12001 ttccacactt tgatcggctt gccgtccatg ccgtagcgag tctggcggaa aataaccggc
    12061 cccggtgagc tcagtttcac cgccagcgca atgcagcaca gcaccgggga gatcagcagc
    12121 aggataagcg tcgccagcac aatatcttcc gcacgtttga gcaggcggtt aaccccggaa
    12181 agcggcgtgt catacagcgg caccaccggt acgccgttca tctcttcgat acgggaatgg
    12241 agaatgttga aggtaaagac atcggggatc agcagcaccg aacaggtggt gtccgccaat
    12301 tgatggacca gttttttcac gcgtgcgccg tcgcacattt gcatcgcgat atagacgtta
    12361 tgaatcttgc ccgctttcgc atcctcgacc agctgttgca gattgcccgc ccagtcgtta
    12421 gaaacgccac ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc taaccacggc
    12481 tgattacgga agctctccat cagcatttgt ccggcggcta aatcccccgc caccgcgacc
    12541 atgcgcttgt tatagccatg attacgcagc cagcccgccc caatgcgaat acacgaacgg
    12601 caaaccacca gtccgatact ggtcagccca taccacgcca gccagatttt cagttgcgtg
    12661 tcgaaatcat tgttgaacgc caccagcccg gcgctgaaaa tcacgcttaa ggtccagttt
    12721 tgtagcagca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg ataaaaatcg
    12781 gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa caggtgcatg
    12841 tagaggaatg acagtccgct gacttcgcaa accagccata gtccggcaaa catgatggtg
    12901 atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc tcgctcgcgc
    12961 ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt ggggtaaggg
    13021 aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc ttccatcagc
    13081 ggcacatcac cgcgcgattc aacattcagg cgcaccaccg gttcggtgtt ggaggtgcgc
    13141 aggttaaagc gccagtcggc aaaggtcatg ctgatgccat cggtgcgatc caccgccagc
    13201 gcctcgcggc taaaatgctg ttcgacgcgg ttaatcgcct caacagggtc cgccagtttg
    13261 ctgttgatct caccgcttgc cggaaatgct gccatccggt cacgcaccag ttcgcccagc
    13321 gttttccctt tcaggcacac cagttcggcg accagcagcc acgggatcat gccgctgtcg
    13381 cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta gatggcgtct
    13441 tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac cggggtgccg
    13501 cctgcggcag tcaccacatc aacggtgttc caggagagac gcggatcgtg gatgatcttc
    13561 gcgccgggat tattttcgag gaacgcttct gccagcaggc cgacaatgta gtagccctcg
    13621 ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc aaaggcaatg
    13681 cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcggca ttccggcagc
    13741 agcgggttag gaattccgtt ggggaaattg ccgtccggcg tgttgtgcac tttgatcaat
    13801 tccactggtg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac cggacccgct
    13861 gcgccgttcc cggagttgat caccagcttg agcggcgtga ggtttttgac gttgatataa
    13921 ccgaacaggt gatcaacgta agcgtcacgc agattgattt gctgatagcg accgcgtttg
    13981 gtttcatcaa cgggaggaaa gtcgttggct tctgccagac gctggacgtc gcgcagtccg
    14041 gtatccccgc tgatcgggcg ggccccctcg cgcacgagtt tcataccgtt gtaatccatc
    14101 gggttatggc tggcggtaac ttcaatgccg ccatccacgc cgagatggaa cgtggcgaaa
    14161 tagatttctt cggtgccgga cataccaata tccagcacat cgacgcccgc atcctgtaaa
    14221 cctttcgcca gcgccagttt taaggtttcg ctggtgaggc gggcatcacc acctaacaca
    14281 atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc gatatcttca
    14341 ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt taattttttc
    14401 atatcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa ttttgttttt
    14461 gtcgttattt acatcgcggt ttcgcattca ttgcctgatg cgacgctgcc gcgtcttatc
    14521 aggcctacaa acccgaaccg taggtcggat aaggcgttca cgccgcatcc gacaaacagc
    14581 gcctgatgcg acgtttacac ccgtccgtag cgatccgcga agcgcaccac atcatcctct
    14641 tcaagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc cgggttttcc
    14701 aggcaatgcg tcgcccccag cggaatataa atggactcgt tttcaccaag gagtttgata
    14761 tcaccgtcga tagtgacttt tgccgttccc gcgaccacta cccagtgttc cgcgcggtga
    14821 tggtgcatct gtactgacaa gccttcgccc ggtttcacgg tgatgcgttt cacctggtag
    14881 cggtcgcccg cgtcgataga gtcatatttg ccccacggac gatacacttc gcgatgcacc
    14941 cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac atcctgcacc
    15001 gcgttacggt cggcaatcag caccgcatct ttggtctgca ccaccaccag atctttcacc
    15061 ccgacggtgg tgaccaggcc agattcggca tacacatagc tgttttcagt tttgtgatta
    15121 atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc ccataaagag
    15181 gaccaggaac cgacatcgct ccagcccgca tccatcggca ccacaacagc atctgccgta
    15241 cgttccatga ctgcgtaatc caccgactct tccgggcagg cgagaaacgc ttcttcatcc
    15301 acacgaataa aatcgagatc cggatcgatg gcgctcatcg ctttttcaca ggcgtcgaga
    15361 atatccgggc gatatttttt cagttcttcg agatagcgtc cggcgcggaa cagaaacata
    15421 ccgctgttcc agtaatattc gccgcttgcc acataggcct gcgcggtttc cagattcggt
    15481 ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccgc agacacttca
    15541 ccgcgacgaa tatagccata tccggtttca ggtagatccg gcacaatgcc gaaggtcacc
    15601 agcttgcccg cttcggcata cggcatggca ttacgcacgg cggtgcgaaa agcgtcttca
    15661 tcggcaatca catgatccgc cgccagcacc agcattaatg ggtcgctctc cgggctatga
    15721 cgttttgccg ccagcgccgc cagcgcaatg gcaggtgcag tgttacgccc tgccggttcg
    15781 agaataatat tctcggtgag tttgttcagt tgacgcagct gttccgcgac aataaagcgg
    15841 tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag gcggcagatg
    15901 gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg gtaaagtacg
    15961 cgggaaagcg gccataagcg gctaccggag ccacctgcca tcacaactgg atagagtttc
    16021 gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg ttctctttat
    16081 cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttgtg tttgggcagc aggagcgcct
    16141 gacgaatccc cgccaccagc gcctcgaccg attccggttc cacgcaaacc gcaatgcccg
    16201 gaaaggtttc gcaaagctgt cccagttcgg tgtgggcttc tgcggtaatc accgcgttac
    16261 cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt
    16321 tttgcaccac cagatggcaa tcgcccatct tcagcagtgc gggtaaagcg tcatacgatt
    16381 gcagcggaaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt tccagccgcg
    16441 ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc aggcgatcgg
    16501 cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag taaagaatga
    16561 tttttttgtt atccggcagg ccaagctggt tacgaagggc atcaacatcg gcatttgcaa
    16621 catgctgaaa acgggcgatt tccgaccagt tggggaagaa gatgacgttt tccgccgcca
    16681 cgcctttttc gatagcttta ttcatcatcg aacgcgaaat cgtggagacg ttatcgacgt
    16741 tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgccg cctttacctt
    16801 ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata tggagcaccg
    16861 tgcgcgcccc agagagtttc gccagcaggc gcattcccgg cgtgcaaaac agcgttggca
    16921 ccacgccaat aatgcgatcc ggctgccagc gacgttgcgc catcaacggg aaaaaactgc
    16981 tgacggcaaa actgccgaga tgcaacaggc gtttcagggt gctcggctgt tttggcacgt
    17041 acagcgggca gcgccacacc gtggcggccc cctcttctcg tttgtaccgc caggcggaat
    17101 agttctcgcc cacctgccat tgcgggtagt aaggcggtgc ggtaataacc cgcacctcat
    17161 gaccttgcgc cgcaagccat tccaccatct cgccggtgta tttgccgatg ccggttaact
    17221 ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc cggcacgctt
    17281 ctcagcgaga aaataggcgc ggctgttagc gtgaacattg tcactggcga gcagtgcgtc
    17341 cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtaacagct cttcttccac
    17401 tactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc cggagaagtt
    17461 atcgtcataa aagtgctgcc agacaccgta aaactggcct gctgttatcg gcagacgcag
    17521 ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt ctttctgcac
    17581 gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt tgccaagcag
    17641 aaactcgccg cgactgttct cgacaataaa gtcgagagag acaagcggtg tggagcgcac
    17701 taccgtggca aagtcttcct gacgtaaaaa catcattacc cccgaaagcg gtcttgattc
    17761 tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc gtgataccag
    17821 ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gtgtaccatc cggtttactg
    17881 gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg cgccagctcg
    17941 cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg ctgggtgttt
    18001 tccagccaga cttcgtgcgc cagctccatg acatgaatgc tcgccgccgc catatcatcg
    18061 acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc cggcgcattc
    18121 tgtgccgtcg cctcgtggaa gcgacgcagc aatgcaggta tcacatggga gttactcggg
    18181 tggaagttgt catgcggccc gtacaggttg gtcggcatga ctgagcggta atcgcgtccg
    18241 tactggcgat tgtaagattc gcacagtttg atcccggcga ttttggcaat agcataaggc
    18301 tcgttagtcg gctccagcgt gccctgcaac aactcgcttt ctgccatcgg ctgttttgcc
    18361 agtttcgggt agatgcagga cgatccgaga aacagcagtt tgttcacgtc gttctgatgc
    18421 gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc cgccggatac
    18481 gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg gtcaatgctt
    18541 tcgctggcaa agaaatcatg caccgcgcgg ctgtccagca ggttcagctc gtcgcgggtg
    18601 cgtaatacca gctctacatc accgcgctgt tcgagctgcc gcctgatggc ggaaccgacc
    18661 atcccgcgat ggccagcaat aaaaatgcgt tgtttactca tgcttatgac tccagcgcga
    18721 tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct tcgaggtcat
    18781 tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag cccagttttt
    18841 cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc cgccggacgg aagtaacgcg
    18901 ggtcaacggc gataatcaca tcgcccggtt taacgcccgg cgcgtcatgc ccggtgacgg
    18961 aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg atgcccaact
    19021 gtgctgccgc catttccacg aactgacgta cggagtactg aacgccggtc gcgataacga
    19081 aatcttccgg ttgttcctgt tgcagcatca tccactgcat ttttacgtag tctttggcgt
    19141 ggccccagtc acgcagggaa tccatattgc cgagatacag gcacgactcc agcccctggg
    19201 cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa agtctcgcca cggcgcgggg
    19261 attcatggtt gaagagaata ccgttacagg cgtacatgtc gtaggattcg cggtagttaa
    19321 cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agagcgtggg tagaacggcg
    19381 tggtctcttt ctgcggaatt tcctgcacca gaccgtacag ttcagaagta gaagcctgat
    19441 agaaacgggt tttcttttcc agaccgagga agcggatcgc ctccagcagg cgcagcgtac
    19501 ccatcgcatc gacgtctgcg gtatattccg gtgactcaaa agaaaccgca acgtggctca
    19561 ttgcgcccag gttgtacact tcatccggct gcacttcacg caaaatacgc gtcaggttgg
    19621 aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg tgcggatcct
    19681 gataaatgtg atccacgcgc tcggtgttga atgacgatgc acgacgctta ataccatgca
    19741 cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt ccggttacac
    19801 cggtgatgag agcgactttt gacatgtctt attcctctgt attttttgaa tttattcagt
    19861 ttcaacgcgt tcgcgtatca ccactgcggg attcccccgg caaaccacat ttgccggaag
    19921 cgatttaaaa acactgcttc gtgcacccac gacggtgccg ttgccgatcg tgacgcctgg
    19981 ggcaacaaag acatcggttg ccagccagca tttctcgcca atcacaatag gcgtggcgtt
    20041 aatggtgaaa tgttgacttg catggtcgtg gctaccggtg cataaataac ttttttgcga
    20101 tatcaccgaa tgtgcgccaa tggttatttc accgagggta tataaattga cgtcatcgcc
    20161 gacccacgcg taatcaccta aggttaattt ccacggatag gtaattttta ctgacggacg
    20221 aataactacg ttttttccta tttttgcgcc gaataaacgt aataaaaatg cccgccagcg
    20281 atacaatact tgtggcgacc aggcaaatat tgttgcctgt actgcccacc acaattgcac
    20341 tttaatagcg ttgccacccc ggaacccttt cggtaccgag aaaccgctta aatcttgcat
    20401 cgttttccct tatattcagc ttttgttata taaggctttc gtctttgagg tagtacgttg
    20461 gcgtaaatgc caggataatt ctgcccagaa gccaggcaca tgtaatattt gccgttggac
    20521 ttttttggcg tcagcacaca attccatatt attggtggtt gataccccac ccatagaaaa
    20581 ttcagacacc aggccattga gttttttaaa tgcataaccg gctttataca ttttggctgc
    20641 cagcgcgtag tcggaagaaa ctttatattc caggtcataa cgccattttt tcaagccgga
    20701 caccgggaaa aatatcgcct gatgactggc gggcaggctg tgataaatat accaacccgg
    20761 tttggcgcta cgtttaattt tatgcccgtc gccaaaatcc agcagcgcat cgccggtgat
    20821 catcatgtta tctttttgca cttttaactt acggacaaaa tttgcggcat cctgatgaaa
    20881 aatatcgcct gagttgagaa acaacgcgaa cttgccatgc gccatcgcaa tgcctttgtt
    20941 catggcgtcg tagataccgt tatctggctc gctgacaaag cgtaggttat agataccatt
    21001 gagattttcc agatactcac gggtgccgtc attggaaccg ccatcgacaa caatccattc
    21061 gaagctgata tcgtcagcct gcgccagatg cgccaacgag gcatgtgttt tgactatccc
    21121 tgcgaggtta cgaaacgcga cagtgattat gctaagcaac atgttcttat tcttacctcg
    21181 taatatttaa cgcttttcgc aaaataaacg gacagacgat taaaaatgca tattccgggc
    21241 taaatatcga accggtaaaa aacagcgata ccggagtaaa aagatataat tgcacgcgaa
    21301 aattgcggtt atcaccaaaa gcgttgatca tcatttttat cactttcccc atgtaccaca
    21361 gcgataaaaa cactgcgaac caggaaaaat aaataatcag cagatacaaa ccattgtcta
    21421 tggtttttcc gacatccgca ccgttaaata ttccgaatga tgcgacatat tcataaagtg
    21481 agccaaatct gactacaccg tcaatatggg tcaaagaata accgaccatc accagcggac
    21541 cgacaatacg ataatacgat gacgatcctt ccgtacctaa atcgcccaga cgggtggaaa
    21601 tataaggaaa cgcgattacc acaccaacca ggaatacagc cagagaaatc aatgctaacg
    21661 gtaacttttt cttaatcgcc tctttattca gatattgaaa tgcccactcc agcaaataaa
    21721 acaggataaa ggtcataacc cctgaaaacg atccggataa tattatccct gcaagaatca
    21781 tagcatcggt tttaggcgtt ttgataccaa actgtttgat gctgagccaa attgagatta
    21841 atgccagagc gaaaaatgcc ggttcgaaat aaagtgctgt cgtgcgcttg ccgccgaatt
    21901 taatgaaatt cagcacatag ctgttgctgt aaatcagata tttcgaaatc gactccataa
    21961 tactgctacc gccggtgagg ataatttgcg ccatctccac cgccgccagc accaccacca
    22021 gcccgaccac cagataaaag aaacgtaata tcttgcggtg gttgtggggc gaaatcgttt
    22081 taaagcgaat actccacacc atgccaataa tgatcacaat atagacaaac agcatagttg
    22141 aagtgacgta tttaccggca tccaacgact gaccaaacag gtagttaaac gccgtgagtc
    22201 ccgcaccaat ccctaaggca atcatcaatt ttttaacgct gatgcgttct aaaaacagca
    22261 gtaacaagac aggtaaaaaa gtgacgatgg tgatgggaaa actttcgcca agctgggcga
    22321 ttttgacgtt aaccagcagg tagatcagcg gcagcagcag gtagctacag attctgatag
    22381 aagttgacat actcctccag catctgttgt ccactgtagg cggcgcggct gcgctggctg
    22441 aactcagcca gcgtggtacc aaatatcgcc tgtgcgattt ccggtttgct tgactgcacc
    22501 agttgcagca catcttcttc gctgacggtt ttaccgccgg atttttgcag cacttcccgc
    22561 gccgcgtcgc tatgggtggc aatcaccggc acgccaatcg ataacgcctc acacaaaatc
    22621 agcgggtagt tatcgacgcg agaactgaac accagcgcat ccatctggtt gagcgcgctc
    22681 atcagtttac gtttgtcagt ttcaaagccg tgattaacaa cgttaccagc ggtgaacggc
    22741 gagaacttac cgaaagtatg cagttcgatt ttgtcgccca gcgccatcat ctcgcgtacc
    22801 agttgctggt tagttttgcc gtcgtaacgc aggtcatgcg ccaccaccgc gattttcggc
    22861 ttgccctggg tttcgcgcac cggaggcaag tccgccagaa tcgcttcggt tgccatatca
    22921 ataccgttat tgataatccg gcaacgccct ggaccgtaca gactattgaa agcgtctgcc
    22981 acatgctggc tgggggaaat aaactgacag cccagcgcca gcatctcgct gaataactgc
    23041 cgtttgcccg ccacccgttg gtgtgcgcga tcaatcttca ccggcggata attatttaag
    23101 gtcgggcatt tctggcagcc cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt
    23161 ccggtaacgc tccagtgatc gtgtaacgtc cagaccagag tgacatccgg tttatggttt
    23221 ttcacttttt cgcaaaagcg caccacgctc ttaagattca accagtagct gtgcagcaca
    23281 tgaaaatgca ggaccaccgg acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa
    23341 ttgccgaaca gatcgcgatt aagcagacga aacaacgcaa tgttcgccat cgcggtcatc
    23401 cgcggcgtat gtttgatgac ctgcggatag ttttgatggc tgacgctctc tttgccgcct
    23461 ttgccgtagc cgtagacaaa atgtgacgcc agcccctgtt gcagcgcgcg ctggtggaga
    23521 tctaacgcca cacctgccgc cccgccttcc gccagtcgca cattgaattg cagaatattc
    23581 atttaattac cttcactcgc gctttttctc ccaccaccag cgcgttgtcc gggacagagt
    23641 cgagcaccac gctgcccgcg cccacggtga cgttgttacc aagcgtgata tcaccaagaa
    23701 taatgacgtt ggcaccgagt tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg
    23761 caccacgatt gccgatagtg acgccgtggc gaatggtgaa atcatccccc gctaccacgt
    23821 ttttattgat cactacggcg taaccgtgat ggatagtgaa acggcggcca atggtcgcgg
    23881 cggcctggat ttcataaccg aaaaagcatt cggtgataat gcgatacagc accagcagcg
    23941 gggccgccca aagattgttg aggacgttct ttttgcgcca caccgaacaa aaatgagcgg
    24001 cgcgataggc aagaaccatg cagcacgggc gtaaactcca gctgttggcg cgcagatctt
    24061 ccagcatctt tagcgccccc gaataccatc agccagacgt ttgccgttac gcaccgacag
    24121 cagcgtcaac aaggtgcgcc aggtcatgcg tttattgcgg atctggtaga gggtaaacag
    24181 ctgatatttt ttgctggcgc ggtcaaattt gtctttgtgc ttacggtaaa agtggaagta
    24241 tccggagaat tttttcggtg acgaggtgat ctgcatttcg ccgtgattga tatgcaggat
    24301 ctgcgtcgcc tcttccactt tccacggttc gccatactcc accaccatcc gcaggaaaat
    24361 gtcgtaatcc tgtgccgctt tcagttcggt atcgaacaaa cactctttga aacgccacgc
    24421 ccaggtaaag acctgattgc caatgatatt gcgtttgtag aacaggcggc gtgaatacgg
    24481 cgatttggga tacagtggca aacttgccgg ttgggaatag acttcgccct ggcaaacgta
    24541 gtcgttagcg tacaaaaagg cgtgtgtgac cagttgctgt ttatgggcga ggaagacgct
    24601 cagacggttg ggtgtccatt catcatcatc atcgatcccg gtgatatatt ccccttgcgc
    24661 cagcataatc gcctggttac gtaccgcgca cgccccgctg ttaatgtcgt tgtgaatgta
    24721 agtgatacgc ggatcgttga gggcggtgac gtactgttgt agttgttccc aggaggtgga
    24781 gcaatcatcc acgatgatca tctcccagtt actgtagtcc tggcgcagga ccgattttat
    24841 cgcccgaatc gccagttgtt gacggttcca ggtcggcata tagattgaaa tcagcggatt
    24901 gtctttcata ggttgttctc ccggagtgtc gagatgttct gccggtttat aagctggttt
    24961 acaggttctg ttgccggatg cggcgtgaac gccttatccg acctacggag cgggtttgct
    25021 tgcctgatgc ggcgtgaacg taggtcggat aagatgcgtc agcatcgcat ccgacagcgg
    25081 atttcgttat ttcgcgtccg acttatattc gtattcgtaa tagccataat cctgatacgc
    25141 gctggcgcga cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
    25201 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
    25261 caacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
    25321 cgtatcaatc aacaccaggt cgtagttttt actcgcccag ttcaccagtt cggcaaaacg
    25381 ttcgctcatc aacagttccg aaggatttgg cggtacctga ccgcgcggga tcaggtcaaa
    25441 tttggcaata gaggtcggtt tagcagcggt agtaatctcg ccctgaccaa tcagaatttc
    25501 cgacaggcca ttgacgttat tagtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
    25561 gcagtcgatc aacaacaccc gtttattggt ctgactgatc accgccgcca ggttggcgca
    25621 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgtgc
    25681 ctgcatcatc gcgaagtgca gactggtgcg cagactacgg atggcttcaa tcgccagatc
    25741 ggttggattc cccaccgcca gtagctggct ctgtttatag cgtttaatcc ctttgatggt
    25801 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
    25861 accgtgttct tccagcacct gcggactttc gataccgcga ttaaacaacg agcgcagcag
    25921 cacacccacg atagagagca tcaggccaag gataatcgcc ccaaggataa tcagcccttt
    25981 cttcggtttt agcacaccag gctgggtgat tgccgggtca acaatgcgca catcgccgac
    26041 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
    26101 ctgcccagac tcgacatcgc gggtcagacg gacaatttcc tgctgggttt tcggcatcgc
    26161 cgttacgcga ccattaagtt tggctttttc gtcttccagc gcctgccgtt tctccagcag
    26221 cgtgcgatac gccgggtgaa ctttggtgta cagcttggag atttccgctt ctttaaaggt
    26281 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttccag
    26341 cggcagatca actgaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
    26401 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
    26461 cgccgatttg cgctcaatat tttgttcctg atagttacgg gcgatactgt taagaatgtc
    26521 gcggatctgt tcgcgatctt ctccagtata agtcaggctc aaaacgccag catctttgcc
    26581 gttctccgtt accgtcaggc tgttttgcag ttgattgatc atccccagcg tggagtattt
    26641 ggtgacggta aactcactgc ccgggctggc gtgaatagct tcaaccatca gcgtgacgcc
    26701 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cgctgctcaa
    26761 agtgtagttt ttgtcatcaa gcacattaag cgtaaacacc tgatccgcca tctccttagg
    26821 acggttaaag gtggtcactt ttaccgtctc gttctggcgt cccatcaggc gatcccagcc
    26881 cgcaccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
    26941 cgttttacca agcaccaggc gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
    27001 tttgttggct aatgccgatc cgatatcctg cactaacgaa ttgccgctgc tttgttcgat
    27061 ttgtaccagt gcgtcggcgc tgtatatcgg cgtagcgaag aaggtgtaaa ccacggcaca
    27121 gagggcaaat acggcggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
    27181 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
    27241 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
    27301 tcaagtaatg tgtacaccgc tacaaacgtt tcccggcttt tgcgatacgg atcggggatt
    27361 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
    27421 tcgcataagc gttcgatatg gcgcttttcc atggtcaaaa tcaggtcgta gttgcgacac
    27481 aggcgacggc tgatttgccg tgcacagtga ccttccagag acagttgatg ttctgcggcg
    27541 acgctgatag cagtagggtc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
    27601 actttcagct ccgggtgata acgttgcagt aagcgttccg ccgtcgggga acggcaaata
    27661 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
    27721 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
    27781 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
    27841 attctgtcgc catcaccagc gatgtggcat cggacatatc cagctggtag atattggcaa
    27901 tcttgccgtt acgcccgccc tcgcctttca acggacgaat gacaaagatg ccgctggcgt
    27961 tggaggtggt catgtcgatg ccttcagcat tacccagggc ttcagttagg gtcatgccgc
    28021 taaagtccat tttcagggtg ctctgttttt taacttcacc catcacaaac actttcagat
    28081 catcattacg cggcacgtag agaatatcgc cggggtaaag cagtcggttc tgattgaggt
    28141 cgccgttttg catcagcgcc tgcaaagaaa tgcgttcttc acgaccattg tgtgttagca
    28201 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcatcgagaa
    28261 tggtcagtgg tacgttggta atcgcctgtt gaccggattt attcacctga cctgagatat
    28321 aggctttttg cgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
    28381 tcgctaagcg accggtaata tcactgcgga tttcagcgag cgttttcccc acgacgtgaa
    28441 ccttgccgat atacgggtag aacatggtgc cgtcaggctg tacccagttg ccggtgtcgc
    28501 tggagctgcg gtactgaccg gctggcgtgg tgagttccgg gtgatcccag acggtgacat
    28561 taagaacgtc ccccggcccg acgcgatact gataattcgc gatctcactt tccagcgtca
    28621 tattggggcg cgctacattc gggcgtgggc gtaattgatc aatcaggcgc ggggtcaacg
    28681 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
    28741 tacccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
    28801 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
    28861 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
    28921 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
    28981 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
    29041 tagctaccaa tacactgatt tagcttaatt tttaaaaccc tctcaacata cagtcgttga
    29101 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
    29161 tattggctct ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
    29221 acaattcggc aattggctat ttttaagaat tatattaagt gtcattcaat atggttttta
    29281 ggagtttctt taggttgaca atatttaata tagtgtctcc acatgcgata tttcttaaat
    29341 aatgttttat tattaccact tttaattcag ggataatgtg aggttataaa cctcaaataa
    29401 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
    29461 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
    29521 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
    29581 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
    29641 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctggcgtca atctcctggc
    29701 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
    29761 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
    29821 ggctggaagg gaaagacagc aataacccca cccaacgcaa aggtgcgaag ttctgggggg
    29881 tggtgacaca aattgtggta ctggacgcca tcttctcact cgactcggtg attaccgccg
    29941 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
    30001 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
    30061 tctgcctaag tttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
    30121 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcgctca
    30181 atcagttggc tatctttaac cggcgacgtt ttctttccgc taaccagaca ctgcgccagc
    30241 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
    30301 ccgaaaccgc gtctatgctg gtggatcatg gtaaccagca gatctttaat ccgcaggaac
    30361 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtcagcagc attatgacgt
    30421 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttac
    30481 tggagcgaaa tcagcatacg cggcttgtcg ttactgatgg cgatgacgca gaagatttgc
    30541 tcggtgttgt tcacgttatc gacctgttac aacagtctct gcgcggcgaa ccgctcaacc
    30601 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttacctgccc
    30661 tggagcagtt ccgtaatgcc cgcacacact ttgcttttgt ggtggatgag tttggctcgg
    30721 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
    30781 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
    30841 cgaatggtca tatgccgctg gaagatctgg tgcaatatgt gccgctaccg ctggatgaga
    30901 aacgcgaata tcacaccatt gccgggctgt tgatggaata tttgcaacgt attccaaaac
    30961 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
    31021 gcgtgcagaa ggtgcagatt attccgctgc ataaggatgg cgagatggag tacgaggtgt
    31081 gataaaacat caaatgcgca ggttcaaact gcgcatttga tttatacaaa atttagtata
    31141 atctaaggat ggagtaatga gaaaaaagct ggcttttctt gataccagtc ttgacgattt
    31201 acgcgcattt ccagaatcgt ctcgacagga aattggctat caactcgaca ggatccaaca
    31261 agggttaaac ccatacgact ggaaaccttt ttcaactatc ggccctggtg tgcgagagat
    31321 tcgtactcgc gatgctgatg ggatctatcg cgtcatgtat gtcgcaaaat ttgaagaagc
    31381 ggtttacgta ttgcattgtt ttcaaaagaa aacacagact accagtcaat cgaatattga
    31441 tttagccaaa cgacgctata aagaacttgt tcaggagcgg aaaaatgaaa attgaaacct
    31501 ttgatagtgt gtgggatgcg gtgagtgaca cccctgaaca agcggaaaat atgcggatcc
    31561 gtgccgaact tgttacgatc attaataact ggattgaaca acaagggttt agccaggctc
    31621 aggcagcgtc tgctttaggg gtcactcaac ctcgcatatc tgaacttgcc agaggtaaga
    31681 tccagatctt tagtatcgat aaattgatca ccatgatggc tcatgcagga ttgcatattc
    31741 aacgtattga agttcaatat ccacatgctg cttagtacta taaatacaga tggcgacaaa
    31801 ttgacgccgc catctgcata acttgtctta catcttctcc agcaacttct tcacatcttt
    31861 gccattgcgg gaatctttat tccgctcggc ccagtcattc aggcgacgtt tcgcttcgtc
    31921 ctgtagatgt ttgcgcagta actgatccac ttgcaggctg taattaagtt gctgccagtt
    31981 gccataaacc cgcagcggta ctggcgtttc tttgaggaaa tcaatcagtt tgctttcccc
    32041 gttccaaccg ccaatcacac gaatgtcaaa ctgggtgtcg caggtttgat ctgccagatt
    32101 caacatgcct tcccctgtca gcgccagcac aggcgattga ccttgcatgt cgtttaacgt
    32161 cacgacgcca tccttcaacg tcaaatcggt ggtaaagcgg tcaagacgcg ttacgttatc
    32221 gaagttttca gcggccttca catcaccacc attacgttct accgcttgct gaatcatctg
    32281 ctggaagttc atcccttcca tgcgcgtgtc ggtcatttcg acatgtgcct gtccttgcca
    32341 gttatggcgg aatgcgtcgg catctatgtc agcaccggag aaatcaccag ccagtgacat
    32401 ctttccggtc aacgaaatcg gatagttaaa cgccttcaga atggtgccaa tctcaacgtt
    32461 ttccagccgt ggctggaagt ttattcgcgg atttattgat gtcgcgtcca gcgtgcccgg
    32521 cagtgaaacc tgtccaccgt taagtttgcc ctgcagttga gtaatttcca gcagacccga
    32581 cttgttggtc atttgcgtgg caacatctgt aaaattcatt ccgcgccagc gcacgttact
    32641 ggcctgcaac aaaatatcag ccgtaaagcc ttgcagtccc tgataggccg gttcatcaat
    32701 acgcgaagaa atgaccgggc gcggcaacgt ggattggctc tgcccctgct gcgcggcacc
    32761 gttttcaccg ttcgctgttt ctttaagcgg gatgaggttg tcaagattca gttgcgggaa
    32821 ttgcagcctc agctgccatt ccggtttctc tgtcagcgtg acctgtgctt gcccgctcag
    32881 cgtactgtca ttggcggtca agctgatttg gttaaatgaa aggcgtttat gtgactcctg
    32941 ccactgggcc tggaaactcc cctgcccctg aataccttgt tttggtaaat ctgcaccctg
    33001 caactgccag ttaacttgtt caatagccgc cgttaaatca tgcggataat cagaagcatc
    33061 taccgtaccg ttaagggata tcgtgagatc gcgctgatcg cgattaaccc gcccggagaa
    33121 ctcaaatgaa ccacgatgtt ggggatcttg ttccatttgc aggcgaatat tgcggattgt
    33181 cacctgctcg tcatcttcat gctggaacac cagcacgctg tccgccacct taagactgga
    33241 tatatcaaac gaccatccgc gatcgtctga cagatccggc aaggtattgt cgcgcggtgc
    33301 aaccggagcg tcttcactgc gcaccgcttc cgtctgcggc gtcagttgga tcactgcccc
    33361 ttttagcatc acctgcttaa cgctcagttg atgactcagc agtggtaaaa gcgccacgtc
    33421 cagacgcatg ttgtctgcgc gaaccagtgg ctggctcgcg ccctgggcag tgagagacat
    33481 tcgcccggag aggatactaa gctgcggcca gacgtgccaa cgcagtggcc cgtcgagctg
    33541 caattgataa ccgctacgtg cagcaacttg cttgaccata tagtcgcgga aatcattcgg
    33601 attcaccagc aacactaacg cagataaccc ggccaccagc acgaccagga gtatcatcag
    33661 cgtcgtcaga aatcgtctca tggtatcctc aatgggcctg aattagtctt tatcgattcg
    33721 actggctacc gcgccctgct ggttgcgata tttcgcatct tcacggcggt tgtaaggtcg
    33781 cgccgccggg ccggaaagcg gctcaaagct cagcgcacca attaacatgc ccggacgcag
    33841 cgccagcggc agcttaccgg agttgtagaa ctccagcaca atacagccag accagcccgg
    33901 atcgatacgg tgcgcggtga cgtgcaccat aagccccaga cgcgccagtg aggaacgacc
    33961 gtccagccat cccaccagat cggctggcag cgtcaccgac tccagcgtca ccgccagcgc
    34021 cagctctcct gggtgaagat aaaacgcctc gctttcatcg agtacgatct catcgctcat
    34081 cacgcggtca agcgcggcgc tcacttcatc tttgggaccg ctcagatcga taaacgcagc
    34141 cgtgtgacca cggaaggtac gaaatttatt gcccaggcgc acatccaccg tcgcgccgtt
    34201 aatacgctcc actggcggac gtgggttgat cgacaaacgg ccttcatcaa gccaggcttc
    34261 aatatctcgg tcacacagac gcatggcatt ttctcctttc gcgcatcact cccttaacgc
    34321 caattacgtc aagggcatac caggttatca ctgaacggta cacaatttat caagcttatt
    34381 caaagaactg acttattttc gctttcaata tatcgatggc gatgcggttt ttcccgccgc
    34441 gcggcacgat aatgtccgca tattgtttag aaggctcaat gaattgcagg aacatcggac
    34501 gcacggtttt ttgatattgc gccatcactg aatccattga acgcccacgc tcgttaacgt
    34561 cacgcttgat gcggcgcatc aggcagatat ccagcggggt atcaacgaaa atggagaagt
    34621 taagttcatc acgcaaacgc gcatccgtca gcagcaaaat gccttcgaga atgatgactt
    34681 tcttcggctc aaccgtcacc gtttctttca tacgcgtatg ttcaacatag ctgtaaaccg
    34741 gcaggtcaat ggccgagccg cgtttcaacg cttgtaaatg ctcaagcaac aggctgtgat
    34801 ccatagcgct gggatggtcg tagttggtct taacgcgttc ttccatcgac agatggcttt
    34861 gatctttgta atagcagtct tcgggaatta cgccgatgtg ttcatcaccg acttgctcac
    34921 gtaattcacg ataaagggta ctggcaataa gactcttgcc ggaagccgat gcgccagcga
    34981 taccgataat gacgcactga tgagactgat cagtcatata tttagcgacc tgattaacct
    35041 ggatgttagg aagggggcga cgaagcgcca aacgcggcaa ttatagggat ttcatccgcc
    35101 tgataccagt cgaatagcgt tgccgcgcgc tcagagttaa ttgttgacga agaattcccg
    35161 gtggcaaatt acgttgatca gttttataca aggtaaaaat tgttatacgc agttgcgcaa
    35221 attatccgcc tttacgtcac tttatgagca attcgcatat aaaatgtaaa acttttgtac
    35281 tagcataaac acagaaacga atactggcga cctggtcttg cggataaagc ggtaatgagc
    35341 aaacaatcac agcatgtatt aattgccctg ccccacccgc tgcttcacct ggtcagttta
    35401 ggtttagtct cgtttatctt tacccttttc tcgcttgagc tttcgcagtt tggcacccaa
    35461 ctcgccccac tgtggttccc gacgtccatc atgatggtgg cgttttatcg ccatgccggg
    35521 cgcatgtggc cggggattgc gctgagctgc tcgctgggaa atatcgccgc atccatcctg
    35581 cttttttcca ccagctcgct gaacatgacc tggacgacca tcaatattgt tgaagccgtg
    35641 gtcggggcag tgctactgcg taaattgctg ccgtggtata acccattgca aaatctggct
    35701 gactggctgc gtctggcact cggcagcgcc attgttccac ctctgttagg gggtgttctg
    35761 gttatcctgc tgacgcccgg agacgatcct ctcagggcat ttttgatatg ggtactgtca
    35821 gaatccatcg gcgcactggc actggtgccg ctgggattgt tatttaaacc acactatctg
    35881 ctgcgccatc gcaacccacg gttgcttttt gagtcgctgc tcacgttagc catcacactg
    35941 acgttaagct ggctttcgat gctgtatctg ccgtggcctt ttactttcat tattgtgctg
    36001 ttgatgtgga gcgccgtgcg cctgccacga atggaagcct ttttgatctt ccttaccacg
    36061 gtgatgatgg tgtcactgat gatggccgcg gatccctccc tgcttgctac gccgcgtaca
    36121 tacctgatga gccatatgcc gtggctaccg tttttgctga tcctgctgcc cgccaacatc
    36181 atgaccatgg tgatgtatgc ctttcgtgcg gaacgcaaac acatttccga aagcgaaacc
    36241 cgctttcgga acgcgatgga atattccgcc atcggcatgg cattagtggg caccgaggga
    36301 caatggctgc aatccaacaa agcgctctgc cagtttctcg gttacagtca ggaagagctg
    36361 cgcggactca cctttcagca actgacctgg ccggaggatc tcaataaaga tctgcaacag
    36421 gttgaaaagc tgatcagcgg tgaaataaac acctattcaa tggaaaaacg ctactacaac
    36481 cgcaatggcg atgttgtctg ggcgttgctt gccgtctcac tggtgcgcca caccgatggt
    36541 acgccgctct attttatcgc tcagattgaa gacattaacg agctaaaacg caccgaacag
    36601 gtgaaccagc aactgatgga gcgcatcacg ctggctaacg aagcgggcgg gattggcatc
    36661 tgggagtggg agctaaagcc gaatattttt agctgggata agcggatgtt cgagctgtat
    36721 gaaattcctc cgcatatcaa accgaactgg caggtgtggt acgagtgcgt actgccggaa
    36781 gatcgccagc atgccgaaaa agtgattcgt gattcgttgc aatcacgctc gccctttaag
    36841 ctggaatttc gcattgccgt gaaagatggc atccgccata tccgcgccct cgccaaccgg
    36901 gtactgaata aagaaggcga agtcgaacgc ctgctcggca ttaatatgga tatgaccgaa
    36961 gtgaaacagc ttaacgaggc attgtttcag gaaaaagagc gcctgcacat tacgctggat
    37021 tccatcggcg aagccgtggt ctgtattgat atggcaatga aaattacctt tatgaatccg
    37081 gtggcggaga agatgagcgg ctggacgcag gaagaagcgt taggtgttcc gctcctgacg
    37141 gtgttgcata ttacttttgg cgacaacgga ccattaatgg agaacattta cagtgccgac
    37201 acctcacgtt ccgcgattga acaagatgtg gtgttgcact gtcggagcgg cggcagctac
    37261 gacgtgcatt acagtattac gccgttaagt actctggacg gcagcaatat aggctcggtt
    37321 ctggtgattc aggacgtcac cgaatcgcgt aaaatgctgc gccagctgag ctacagcgcc
    37381 tcccatgatg cactgacgca tctcgccaat cgcgccagtt ttgagaagca actacgcatc
    37441 ctgctgcaaa cggtaaacag tacacatcag cgacatgcac tggtgtttat cgatcttgat
    37501 cgctttaaag cggtgaatga cagcgccggg cacgccgctg gcgacgcttt actgcgcgaa
    37561 ctggcgtcat tgatgctgag tatgctgcgc tccagcgacg tgctggcgcg actcggtggc
    37621 gatgaatttg gtctgctgct gccagattgt aatgtcgaaa gtgcgcgttt tatcgctaca
    37681 cgcattatca gcgccgtgaa tgactatcac tttatctggg aaggacgtgt acatcgggta
    37741 ggtgccagtg ccgggattac cttgattgat gacaacaatc atcaggcggc tgaagtgatg
    37801 tcgcaggctg atatcgcctg ttatgcctcc aaaaatggtg gacggggccg ggtgacggtt
    37861 tacgaaccgc agcaagctgc cgcacatagc gaacgggcgg tgatgtcgct tgatgaacag
    37921 tggcggatga ttaaagagaa tcagttgatg atgatcgccc acggtgtcgc ttcgccgcgg
    37981 atcccggaag cgcgtaattt gtggctgatt tcacttaagc tctggagttg cgaaggcgag
    38041 attattgatg aacaaacatt tcgtcgtagc ttcagcgatc cggcacttag ccatgctctt
    38101 gaccgacggg tattccacga ttttttccag caggccgcaa aagcgattgc cagtaaaggc
    38161 ttaagcatcg ccctccccct ttccgttgcc ggtttgagta gcgccacgct ggtgaatgaa
    38221 ctgcttgagc agctggaaaa tagccctcta cccccacggt tattacatct gattattccg
    38281 gcagacgcga ttttagatca cgcagaaagc gtgcaaaaac tgcggctggc gggatgtcgg
    38341 atcgtattca gtcaggtggg ccgcgatctg caaatcttca actcgctgaa agcgaatatg
    38401 gcagattacc tgctacttga tggtgagtta tgcgccaatg tgcagggaaa tttgatggat
    38461 gagatgctga ttacgatcat tcaggggcac gctcagcggc tcgggatgaa gaccatcgcc
    38521 gggccagtag ttttacccct ggtgatggat acgctttctg gcatcggcgt cgatctgatt
    38581 tatggcgatg tgattgccga tgcccaaccg ctggatttgc tggtgaatag cagttatttc
    38641 gcgattaact gaggcgtgct tctccatcgc ctgatacgac gctaacgcgt cttatcatgc
    38701 ctacaaatcg ctcattcccc aggccggata aggcgctcgc accgcatccg gcgaccaacg
    38761 tcatgcttcg tctggttgcc agccttccgt ataccagata tgcaacagcg cataagaacg
    38821 caagggcttc cagcgttcgg cataacggcg gatttgtgcc ggtgtcattc ccggaaatcg
    38881 ctgtttaatc agataatcat ccggcaggaa aacatctttc gcctgccagc cgcgcaaggc
    38941 gaaataattc gccgtccagc gcccgatacc cggaaaagtt tgcagcgttt tcatcgcctg
    39001 ctccacatcg cccggtattg tcattggtaa ggtgccctcc agcgccgcat ttgccagatg
    39061 aatcagcgcc tctgcccgtt tcaacggcat acctaacgct tttaatgcct gcggatctgc
    39121 tgctgccagc cgctgaggcg tggggaagca gacataatcc ggaaaatcat ccagcctttc
    39181 gccataaagc tgtgccactc tggcggtcaa ttttgccgcc atcgccacgc tcaccagctg
    39241 gcctaaaatc gcccgcacgc cctgctcaaa agcatcaaca caaccgggta aacgcaatcc
    39301 gggccgtgcc gcgcctaact tgcccaacgc accgttaaca atctgcgggt tacattgcag
    39361 atcaaacagg cggctcattt tcgccagaca ctctgcggca acgggttcta aacctgcact
    39421 taaatttatg tgcagagtat ggctggctat atccggaata gcagtcacca cgccgcgaca
    39481 ttcgcccacc gccagactac gggcataata atcgtcagcg accgtttcca caccgttcac
    39541 cgcacgggcg gcgagaaatc ccaacatcca cgaccagtca tacggcggct gccagttcag
    39601 ggtatacatc gcatctcctt gttaatccgc tttcagcata aacgttattc agacgctacg
    39661 ctttgctttc atattccggt tgtcgcgacg gcaacatttc gctaaagtca cgccccttct
    39721 tcaccggcat ggggattatt tcgtgtttat tggttttgat tacggtacag caaactgttc
    39781 agtggcggtc atgcgcgacg gtaagccgca attactgaaa atggaaaacg acagcacgct
    39841 gctgccttca atgctttgcg cgccaacgcg tgaagcggta agcgaatggc tgtaccgcca
    39901 tcatgatgtt ccggcagacg acgatgaaac gcaggcgctg ttgcgtcggg cgattcgtta
    39961 taaccgcgaa gaagatatcg atgttacggc gaaaagcgtg cagttcggtc tttcctcact
    40021 ggcacagtac attgatgatc cagaagaggt gtggtttgtg aaatcaccaa aatcattcct
    40081 cggtgccagc ggcttaaaac cgcagcaggt agcgctgttt gaggatctgg tctgcgcaat
    40141 gatgttgcac attcgccagc aggcgcaggc gcagctgccg gaggcaatta ctcaggcggt
    40201 gattggtcgc ccgatcaact tccaggggct gggcggcgat gaagcaaacg cccaggcgca
    40261 agggattctg gaacgcgcgg cgaagcgtgc cgggtttaag gatgtggtat tccagtacga
    40321 gccggtcgcg gctgggctgg attacgaagc caccttgcag gaagaaaaac gggtgctggt
    40381 ggtggatatc ggcggtggta cgactgactg ttcattgctg ctgatggggc cgcagtggcg
    40441 ttcgcgtctc gatcgtgaag ccagcctgct gggtcacagt ggttgccgta ttggcggtaa
    40501 cgatctggat atcgcactgg cgtttaaaaa cctgatgccg ttactgggca tgggtggcga
    40561 aaccgaaaaa ggcatcgccc tgccgattct gccgtggtgg aatgcggttg ccatcaacga
    40621 cgttcctgcg caaagtgatt tctacagtag tgccaacggt cgtctgctta acgacctggt
    40681 acgcgatgcc cgcgaaccgg aaaaagtggc cctgttacag aaagtctggc gtcagcgttt
    40741 aagctatcgc ctggtacgta gcgcagaaga gagcaaaatt gcgctttcaa gcgtagcgga
    40801 aacccgcgcc tcactgccgt ttatcagcga tgaactggct acgctgatta gccagcaagg
    40861 gctggaaagc gccctcagcc agccactggc gcggattctg gaacaggtgc aactggcgct
    40921 ggataacgcc caggaaaaac cggatgttat ctatctgacc ggcggtagcg cccgttcacc
    40981 gctgattaaa aaagcgctga cagaacagtt gccgggcatt ccgattgcag gtggcgatga
    41041 ctttggctcc gtcaccgccg ggctggcacg ctgggcggaa gtggtgtttc gttaattgaa
    41101 aactggctca gcaacggctt aaatcagcct gcgcctgagc cagtttttct gccgcctgac
    41161 gtagcggttc catcaacggc atggtccgtg ctggagcatg aaggcggaat cgctgcaact
    41221 ctgtcgcacc gacattcagc gccgattcca gccgatgtcg tttggcggcc atttttgcca
    41281 ttaccgcttg tctgtctgca ggcgttaccg cttcgttcgg cctgaacaca aaacggcgtt
    41341 cacagctcgc tttccagtcg ataaccgcct gggtcagatg atcaccaaaa cctttcactt
    41401 gcttaacgct ccgtcgggta acatccgctg ctgtttcaat accaaaggac cgtaacgccg
    41461 ctttacgcgc agggccaacg ccgggaatag aggcaacatc aataaaaaat ccctccagaa
    41521 acttctgctt ctgccgttcc cttgcggtgt cctgaagtgc tgccagatcg cgcttttctt
    41581 cttccggtaa cccaagaatt tcgtccttca ttttttcgag catcgtccgt ttggcgataa
    41641 aaccttccag cccgcccaac tgctggatct ggctgactaa atggtcatag tccattttcg
    41701 cgcgattaaa cgcctcacgg cgttgctgga tttctgcttt gtacgctttg cttgtcaggc
    41761 tgccaataat ccagatagcc gccagcacag gaaccaaaat aatataacgt ggttctgtct
    41821 gaaggccgca aagcagcagc gataacgctg acagtgcgat ctcaatcaga atgatgtatt
    41881 cacgccttaa caggcctaaa ggaagcggcc tgccagtcgg ttggaaatga tcgggtaatg
    41941 gtaattgcaa tgctggcggt gctactgacg ccatcaccat cgcccagact ttcgccagca
    42001 caaaatcacc gctggtggta atgacctctt cgccgagatc aataaaatag ataacgcctt
    42061 gattatccag agcacaccac gggcagtcag ccaaatgacc gggataaaca tgcattgccg
    42121 aaacggtaca tttctttaac tgttggcgta gtaaatcaag tgccgctacc cacgcttttg
    42181 ccgtcggacg cccggttgcc acgccacttt ccgtgaatgc ctgctgaaac atggcttcaa
    42241 catgcccggt aacatcgaca gcggaataga tcgtggcggc ggttttaaac cacgtcgctg
    42301 attatctgac gcgtaggcat aacggaaatg ggcaatatcc gtctccagtg cattacccgc
    42361 atcagagaca agcggtacac cggaataagg atgccgccca ccaaacaaga cgtgaaaaat
    42421 cagcaacgca aggccaaaat tatcgtgatt cgcggtgcgt tcaaagccga caaatgatgg
    42481 caaggtttgt tgctctggcg gcgtaaaatg cgacacgccg acttcgcata aatgcagcgt
    42541 gccattggcg ttaatctgaa aggagtcgct atcaatcaac accactttgc tgtcgcgacc
    42601 taccataaag ctgttctggt ttacgtcacc cacgacgtgc ccgtgctcgt gaatcgtagc
    42661 aaaagatgaa gcaatattgc gcgcaacata gagtagaaaa tcccacgcac aatgagggta
    42721 acgttgacga cgatgtgccg ggctatagat catatgaatc ggttctttac cagaaacttt
    42781 tggcatcata aaaccgatta cttttccgcc tcgtccaccg tgaagcgttg cctgcggcca
    42841 ggcgacataa ttcaacaact gcgcgtcagc tgtcgcagcc ataaaggcaa gtttgtcctg
    42901 ttttaaggcg ggtggcggcg tgtgataaat cttggcgacg ctatcgacaa actcctcgat
    42961 atcataaacc gcgccttcac cgcctttgcc cagttcacag cctggcgtaa cgcactcacc
    43021 agtagcagta tataaagtgg gtttcatggt aaccacagcg ccagtgccaa cgttttatca
    43081 tcatcggtac gctcgtttac cgccgggccg gacaaaaact gctttaacaa ttcaggtaat
    43141 aaatcgagtt gttcctgcgt tgctgacgcc agcccattga aaaacggggt aaagaaaggc
    43201 acatgcggag aattatccag catatttaac gccagccgct ggataccatc cgtaaatgct
    43261 gcaactttgt gcgcacgctc agtgctggtg aaagtttcca gtctggaaac ggcatcttca
    43321 tcggtaatga agtgcgtcat attggtatat tcacccacca tcggcgtgag cggcagttgc
    43381 aaaccgtgcc ccaaatctac caccacgccg ccatcgccaa tctgcataat caaggtgcca
    43441 ttagctgatg aaatcaatcc caaaaacgtg caggcaaaat cgcgaaccgc taattctttg
    43501 gcttccgcct cggcaaaaag tcgctgacga atggtcagca caatgtccgt tgcgagaaca
    43561 tcattaagcc ccaattcccc gccctgcact ttttgcgaca tataagccat cgcttcattg
    43621 acggcgagca tcgcaccttc gcctccctgc gaggcgctac ctgcgccatc agcgacaaat
    43681 accgacaata atggctgctg gtcatttaac caggcaacct gcatctggca ggcatcctgg
    43741 caaggcaagt ccgcgctgat gtgcgacgtt ccaacggttg atgcatagac cagacgccag
    43801 ctcacactga cgtccagcct tttggcgctt ccagcacaac ttccgttccc ggcgtggagc
    43861 gggaaaccga acgcagcgaa ctggataacc agctaaatag ttcgcggaat tgtaatcctt
    43921 gaagaggcaa aggctgacga acgctgattt gggccagcgt cttcatatcc gccccctgaa
    43981 caccgatgga aaagaaggca aacttcttat cttcttcccc ctgaaacacc ttgttggcag
    44041 cagcctgcca ctcatcggtt ggtgcgccat cggtaatcag gaaaatccac ggacgataat
    44101 aggaaatacc attggcgcga tactcacgtt tgcgctcctc aaccatatcc agggctttgg
    44161 taatggcggc ccccattggt gtatcgccct gggcaaacag gatgggcggg aaaaaattag
    44221 cggcgctggt aaatggttgt tccacatgca ccgggccgaa cgtcacaatg ccgagttcca
    44281 ctctttttaa ggccagcgga tcggcaagca gctcatcgcg aaaggtaacc aatccggcgt
    44341 taagttcatt aataggtcta ccgttcatag aaccagaaac atccaacagc aaaatgcagg
    44401 ggcaacgtgg ttccgggtta ctggcaaaat cgctggtggc aaacgtgatt tgttcgctca
    44461 taagaaatcc tttcttagag aattattgag gcccggtatc ataccgactg caacaatgaa
    44521 ataagagcaa acgaagaaac tttcagaatt aggcccggcg gcgcagaaaa ttttgctatt
    44581 cgcctcgcaa gaacggagag ccgtcgattg acactaaggg cggagtgaca taatttcagg
    44641 agtgagggtt agggagaggt ttccccctcc ccctggtgtt cttagtaagc ctggaagcta
    44701 atcactaaga gtatcaccag tatgatgacg tgcttcatca taaccctttc cttattaaaa
    44761 gccctcttct ccgggagagg gtttcccgtt tcagcgtccc gctgaaatcg tcggcttacc
    44821 tcctttcgcc ctgaatgcag tctatcgcta acgcgtgaac aaaatagttt cctctgtgat
    44881 tattgcgtaa cgactcgcaa agaatatttg ctggcaggat cgcagactac aaagcctgcg
    44941 gattgacaat cttatcgtga aggcataatt tcaggagtga gggtagagcg gggtttcccc
    45001 cgccctggta gtcttagtaa gctggccagc taataactaa gagcaccacg atgatgagta
    45061 gcttcatcat gaccctttcc ttatttaagg ccccttcctc gggaggggct ttcccgtttc
    45121 agcgtcccgc tgaaatcggc ggcctacctc ctttcgccat gcaagcagtc tatcgctaac
    45181 ttgcagataa aatagtttca catgtgatta ttgcgtaacg tctcgcaaag aatatttgct
    45241 ggcaggatcg cagactacaa agcctgcgga ttgacaatct aattgtgaag gcatactttc
    45301 aggagtgagg gtagagcggg gtttcccccg ccctggtagt cttagtaagc cgggaagctt
    45361 atgactaaga gcaccacgat gatgatccac ttcatcatga ccctttcctt atttaaggcc
    45421 ccttcctcgg gaggggcttt cccgtttcag cgtcccgctg aaatcatcgg cttacctcct
    45481 ttcgccctga atgcagtcta tcgctgactt gcggataaaa tagtttcaca tgtgattatt
    45541 gcgtgacgtc tcgcaaagaa tatttgctgg caggatcgca gactgcaaag cctgcggatt
    45601 gacaatcgga ccgtgaaggc atactttcag gagtgagggt agagcggggt ttcccccgcc
    45661 ctggtagtct tagtaagctg ggtagctaac gactaagagc accacgatga tgagtatctt
    45721 catcatgacc ctttccttat ttaaggcccc ttcctcggga ggggcttgcc cgtttcagcg
    45781 tcccgctgaa atcgtcggcc tacctccttt tgccctgaat gcagtctatc gctaacgcgt
    45841 agataaaata gtttcctgtg ttattactgg atgggtgctc gcaaatgtgc ccgccattca
    45901 gacgattccc gatagtgttt cataattcct ccatttttct ccctgattgg ctggctacac
    45961 tagtatcatt ccgcgaatcg tttcaggaag agaaactctt aacgatgaaa agcagttata
    46021 aatcccgttg ggtaatggta atcgtggtgg ttatcgccgc catcgccgca ttctggttct
    46081 ggcaaggccg caatgactcc cggagtgcag cgccaggggc gacgaaacaa gcgcagcaat
    46141 cgccagcggg tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga
    46201 ccgccgtaga acaggcagtt ccgcgttacc tcaccgggct tggcaccatt accgccgcta
    46261 atactgttac ggtgcgcagc cgcgtggacg gtcaactgat ggcgttgcat ttccaggaag
    46321 gccagcaggt caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg
    46381 cattagcaca agcccagggc caactggcaa aagataaagc cacgcttgcc aacgcccgcc
    46441 gtgacctggc gcgttatcaa caactggtaa aaaccaatct cgtatctcgt caggagctgg
    46501 atgcccaaca ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg
    46561 tcgccagcgc gcagctgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg
    46621 ttggtctcaa gcaggttgat gttggtaacc aaatctccag tggtgatacc accgggattg
    46681 tggtgatcac ccagacgcat cctatcgatt tgctctttac cctgccggaa agcgatatcg
    46741 ctaccgtagt gcaggcgcaa aaagccggaa aaccgctggt tgttgaagcc tgggatcgca
    46801 ccaattcaaa gaaattaagt gaaggcacgc tgttaagtct cgataaccaa atcgatgcca
    46861 ctaccggtac gattaaagtg aaagcacgct tcaataatca ggatgatgcg ctgtttccca
    46921 atcagtttgt taacgcgcgc atgttagtcg acacagagca aaacgctgtg gtgatcccaa
    46981 cagccgccct gcaaatgggc aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca
    47041 aggtcagcaa acatctggtg acgccgggca ttcaggacag tcagaaagtg gtgatccgcg
    47101 caggtatttc tgcgggcgac cgcgtagtga cagacggcat tgatcgcctg accgaagggg
    47161 cgaaagtgga agtagtggaa gcccagagcg ccaccactcc ggaagagaaa gccaccagcc
    47221 gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg
    47281 cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat
    47341 cttactcgcc gggattatcg gttatcgagc cctgcccgtt tcggcgctgc cggaagtgga
    47401 ctatccgacc attcaggtag tcacgctcta cccaggtgcg agcccggatg tcatgacctc
    47461 tgccgttacc gcgccgcttg aacgccagtt tgggcagatg tctggcctga aacagatgtc
    47521 gtcgcaaagt tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct
    47581 cgatgtcgcc gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag
    47641 cgatctgcct aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac
    47701 gctcgctgtc acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg
    47761 cgtcgcgcag aaaatctcgc agatttccgg cgtcggcctg gtgacacttt ccggcggtca
    47821 gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg gcctgaccag
    47881 cgaaaccgtg cgcaccgcca ttaccggcgc taacgtcaac tcggcaaaag gtagcctcga
    47941 cggcccttct cgtgcggtta cgctttccgc taacgaccag atgcaatccg ccgaagagta
    48001 tcgccagcta atcatcgcct accagaacgg cgcgccgatt cgtctgggcg atgtcgcaac
    48061 cgtagagcaa ggtgcagaaa acagctggct tggcgcatgg gctaacaaag aacaggccat
    48121 tgtgatgaat gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcattcg
    48181 gcagatgctg ccacagctca ccgagagtct gccgaaatcg gtgaaggtga cggtactttc
    48241 tgaccgtacc accaatattc gcgcatccgt ggatgatact cagtttgaat tgatgatggc
    48301 tatcgcgctg gtagtcatga ttatctacct gtttttgcgt aatattccgg cgaccatcat
    48361 tcccggcgtc gcagtgccgc tgtcgttaat cggcaccttc gcggtcatgg tgtttctcga
    48421 tttttcaatc aataacctga cgctgatggc gttaactatc gccaccggat ttgtggtcga
    48481 tgacgccatc gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgct
    48541 ggcggcggcg ctcaaaggcg caggtgaaat cggttttacc attatctcgc tgaccttctc
    48601 gctgattgca gtgctgatcc cgctactgtt tatgggcgat atcgtcgggc gactgttccg
    48661 cgaatttgct attactctgg cagtcgcgat tttgatctca gccgtggtgt cgctgaccct
    48721 gacaccgatg atgtgtgcgc ggatgctcag ccaggagtcg ctgcgtaaac agaaccgctt
    48781 ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc agggacttgc
    48841 gaaagtgctg aatcatccgt ggctgacctt aagcgtggcg ctcagcacgc tgctgcttag
    48901 cgtgctgttg tgggtgttca ttccgaaagg tttcttcccg gtacaggaca acggcattat
    48961 tcagggcact ttgcaggcac cgcaatccag ctcctttacc aatatggccc agcggcaacg
    49021 ccaggtcgcg gatgtgattt tgcaagatcc ggcggtgcaa agtctgacct catttgttgg
    49081 cgttgatggc actaacccgt cgctgaacag tgcgcgtttg cagatcaacc tcaaaccgtt
    49141 ggatgaacgt gatgatcggg tgcaaaaagt catcgcccgc ctgcaaacgg cggtggataa
    49201 agtgccgggc gtcgatctct tcctgcaacc aacgcaggac ctgaccatcg atactcaggt
    49261 cagccgcacc cagtaccagt ttaccttgca ggccacgtca ctggatgcgc tcagtacctg
    49321 ggtgccacag ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga
    49381 ctggcaggac aaagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct
    49441 ggggatcagc atggcggacg tcgataacgc cctttacaac gcgtttggtc agcgactgat
    49501 ttccactatt tatactcagg ccaaccagta tcgcgtggtg ctggagcaca acaccgaaaa
    49561 tactcctggg cttgcggcgc tggatactat tcgcctgacc agcagcgacg gcggcgtggt
    49621 accgctaagc tcaattgcca aaattgagca gcgttttgcg ccgctttcca tcaaccatct
    49681 ggatcagttc ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga
    49741 tgcggtgcag gcgattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac
    49801 gcagttccag ggcagcaccc tcgccttcca gtcggcgttg ggcagcactg tctggctgat
    49861 tgtcgcggcg gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca
    49921 cccgatcacc attctctcga cgctacccac cgcaggggtc ggcgcactgc tggcgttact
    49981 gattgccggt agcgagctgg atgtgattgc gattatcggc attattttgc tgatcggtat
    50041 cgtgaagaag aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg
    50101 catgtcgccg cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat
    50161 gaccaccctg gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc
    50221 ggaactgcgt cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct
    50281 gacgctgttc accacgccgg tgatttatct gctattcgac cgcctggcat tgtggaccaa
    50341 aagccgcttt gctcgtcatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta
    50401 ccgcccggtg gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggttt
    50461 ccgtatgctg ccagtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc
    50521 ctcgctgccc ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggaacg
    50581 ttcgcttgga cgcattgccg gggtcagtga aatgacctcc agcagttcgc tcggcagcac
    50641 gcgcattatt ttgcagtttg attttgaccg cgatatcaac ggcgcagcgc gcgatgtgca
    50701 agcggcgatc aacgccgcgc aaagtttgct gcccagcggg atgccaagcc gtccgaccta
    50761 tcgcaaagcg aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta
    50821 ttcgcagggt gaactgtacg atttcgcctc gacgcagctg gctccgacga tttcgcaaat
    50881 cgacggtgtc ggtgatgtcg atgttggcgg cagctcactg cccgccgtac gcatcgggct
    50941 gaatccgcag gcgctgttta atcagggcgt gtcgctggac gatgtgcgca gcgccatcag
    51001 caatgccaac gtgcgtaagc cgcagggggc gctggaagat gatacccatc gctggcagat
    51061 ccagactaac gatgaactga aaaccgccgc cgaatatcag ccgttgatta ttcattacaa
    51121 caacggcggc gcgcttcgtc tgggcgatgt ggcgacggtg accgactcgg tgcaggatgt
    51181 gcgcaacgcc gggatgacca acgccaaacc ggctattttg ctgatgatcc gcaagctgcc
    51241 ggaagccaat attatccaga cggttgacag catccgggca aaattaccgg agttgcagga
    51301 gaccattccg gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgcgc
    51361 ctcgctggag gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt
    51421 ctttttattc ctgcgctcgg gtcgcgccac tattattccc gccgttgcgg tgccggtttc
    51481 gctgattggt acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt
    51541 aatggcgctc accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa
    51601 cattgcccgt cacctggaag cggggatgaa accgctacag gccgcgctgc aaggcactcg
    51661 cgaagtcggt tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt ttctgccgct
    51721 gctgttgatg ggcggattgc cgggtcgcct gttacgcgag tttgccgtga cgctttctgt
    51781 cgccattggt atttcgctgc tggtttctct gacgctaacg ccaatgatgt gcggctggat
    51841 gctaaaagcc agtaagccgc gcgagcaaaa gcgactacgt ggttttggtc gcatgttggt
    51901 agccctgcaa caaggctatg gtaagtcgct gaaatgggtg cttaatcata cccgtctggt
    51961 gggcgtggtg ctgcttggca ccattgcttt gaatatctgg ctgtatatct cgatcccgaa
    52021 aaccttcttc ccggagcagg acaccggcgt gttgatgggc gggatacagg cggatcagag
    52081 tatttcgttt caggcgatgc gcggtaagtt gcaggatttt atgaaaatta tccgtgacga
    52141 tccggcagtg gataatgtca ccggatttac cggcggttcg cgggtgaaca gcgggatgat
    52201 gtttatcacg ctgaagccac gcggcgaacg cagcgaaacg gcgcagcaaa ttatcgaccg
    52261 tctgcgcgtg aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacagga
    52321 tattcgcgtt ggcggacgtc agtcgaacgc cagctatcag tacacgttgc tgtccgacga
    52381 cctggcggca ctgcgcgaat gggagccgaa aattcgcaaa aaactggcga cattgccgga
    52441 actggcggac gtgaactccg accagcagga taacggcgcg gagatgaatc tggtttacga
    52501 ccgcgacacc atggcacggc tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa
    52561 cgccttcggt cagcgacaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt
    52621 gatggaagtg gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat
    52681 caataacgaa ggaaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc
    52741 tccgctgtcg gtgaatcatc agggattatc ggcagcctcg accatctcgt tcaacctgcc
    52801 gaccgggaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    52861 tgtgccttcg acggtgcgcg gtagttttgc cggcacggcg caggtgttcc aggagacgat
    52921 gaactcgcag gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat
    52981 cctttacgag agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt
    53041 tggagcgctg ctggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg
    53101 gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    53161 tgaagcccaa cggcacggta acctcacgcc gcaggaagct attttccagg cctgtctgct
    53221 gcgttttcgc ccgattatga tgactaccct ggcggcgctg tttggcgcgc tgccgctggt
    53281 attgtcgggc ggtgacggtt cggagctgcg gcaacccctg gggatcacca ttgtcggcgg
    53341 tctggtcatg agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    53401 ccgtctgcga ctgcgttttt cgcgtaaacc taaacaagcg gtaaccgagt aaatgacaga
    53461 tcttcccgac agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc
    53521 gctggacacc accatcgtta acaccgcgct tccctcaatg gcgcaaagcc tcggggaaag
    53581 tccgttgcat atgcacatgg tcattgtctc ttatgtactg accgtggcgg tgatgctgcc
    53641 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    53701 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    53761 ggcacgcgcg ttacagggcg ttggcggcgc gatgatggtg ccggtcggca ggttgacggt
    53821 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgctgcccgg
    53881 tcagatcggg ccgctgcttg gcccggcgct cggcggtctg ctggtggagt acgcatcgtg
    53941 gcactggatc tttttgatca acattccggt ggggattatt ggcgcgatca ccacactgat
    54001 gttaatgccg aactacacca tgcagacgcg gtgctttgat ctctccggct ttttattgct
    54061 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    54121 gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct
    54181 gcacgccaga aataacaact gcgccctgtt cagtctgaaa ctgttccgta ctcgtacctt
    54241 ttcgctgggt ctggcgggga gttttgccgg acgtatcggt agcggcatgt tgccctttat
    54301 gacgccggtt ttcctacaaa ttggccttgg cttctcaccg tttcatgccg gactgatgat
    54361 gatcccgatg gtgctcggca gcatggggat gaagcgaatt gtggtacagg tagtgaatcg
    54421 ctttggttat cgtcgggtac tggtggcgac cacgctgggt ctgtcgctgg tcaccctgtt
    54481 gtttatgact actgctctgc tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    54541 agggatggtc aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc
    54601 ggacaatctg gcgagcagcg gcaacagtct gctgtcgatg attatgcaat tgtcgatgag
    54661 tatcggcgtc actatcgccg ggctgttgct ggggcttttt ggttcacagc atgtcagcgt
    54721 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat
    54781 cgcccttccg gcgtttgtct ttgccagagt gccgagcgat acgcatcaaa atgtagctat
    54841 ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    54901 tttctggcga ttttcgccac ctgcattgtc ttattgatca cgatgcactg ggcggtacgt
    54961 atcagttttg agcgcggctt tatcgattac atcaagcatg gtaatgaaca acggctgcaa
    55021 atgctcggcg atgcgcttgg tgagcagtac gcccagcacg ggaactggcg cttcctgcgt
    55081 aataacgatc gctttgtatt tcagatccta cgttcactgg agcatgataa caacgaagat
    55141 aagcccggcc ccggtatgcc accacacggc tggcgcacgc aattttgggt ggttgatcaa
    55201 aataataaag tgctggttgg cccgcgagca ccggttccac ccgacggcac acggcggccc
    55261 attatggtca atggtgcgga agttggcgcg gtgatcgcct cccctgttga acgactgacc
    55321 cgtaatactg atatcaattt tgacagacaa cagcggcaaa ccagttggct gattgttgct
    55381 ttatctacct tattagcggc gctggcgaca ttcccactgg cgcgcggttt gctggctccg
    55441 gtaaaacgac tggtggatgg cacgcacaaa ctggcagcgg gtgatttcac tactcgcgta
    55501 gcccccacca gcgaagatga gttgggcaga ctggcagagg atttcaatca actcgccagc
    55561 acactggaga aaaaccagca gatgcgccgc gattttatgg ccgatatttc tcatgaactg
    55621 cgcacgcctt tagcagtact gcgcggcgaa ctggaagcca ttcaggatgg tgtgcgtaaa
    55681 ttcacgccgg agacgctggc ttctttacag gcggaggtcg gtacactgac caaactggtt
    55741 gacgatcttc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    55801 gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg atttgccagc
    55861 cgtggcctga aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgat
    55921 cgtttaatgc agttgttcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    55981 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    56041 agcgcgccgg gcgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    56101 gaaggttccc gcaaccgtgc cagcggcggt tccgggctgg gactcgcgat ttgcctgaac
    56161 attgttgaag cacataatgg ccgcattatt gctgcccatt cgccttttgg cggggtaagc
    56221 attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa
    56281 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg ggacagttgc
    56341 tcattgatta tctgcgtgcc gcgagctatg cgccgacgct tatcagccac ggcgatcagg
    56401 tactgccgta tgtgcgccag acaccaccgg atctgatcct gttagatctg atgctccctg
    56461 gcaccgatgg cctgacgctg tgccgggaaa ttcgtcgttt ttctgacatt ccgatcgtga
    56521 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg
    56581 actacatctg caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc
    56641 gccgttgtaa accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgatgatcg
    56701 acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat
    56761 ttcgtctgtt gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcaattgc
    56821 tcaatcatct ttatgacgac taccgcgtag taactgaccg caccatcgac agccacatta
    56881 aaaacctgcg tcgcaagctg gaagctctgg acgaagagca gtcttttatc cgcgccgtct
    56941 acggtgtcgg ttatcgctgg gaagccgacg cttgccgcat cgtttagttt tagcgacatg
    57001 actttgttag ccggagatgg cgttcagatc agatccggca acattatccc acgtatggtc
    57061 agcaaactga cattatctct ccgccctggc aggcaccaga aaatcgctca tactttaatc
    57121 ggtaaacagc acctttagat gctgttttcg atacgcaatt tcaatcaagg agtcattatg
    57181 gctggttggt ttgaactcag taagagcagt gataatcaat ttcggtttgt gctaaaagcg
    57241 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    57301 ggcatcgcgt cggtgcgtag caacagcccg caagaagaac gctatgagaa aaaaacggca
    57361 agcaacggca aattctattt caatctgaaa gccgccaatc atcaaattat cggctccagc
    57421 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc
    57481 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgcgtgt tgttggcgtc
    57541 cggcgcttta ccagctcccc cctacagcgc tacaatgccc gcccttaaag tgggggcact
    57601 cccctggccg cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg
    57661 tttaaaccgg aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct
    57721 tatggcgcag atgctgttta tgcgggccag ccgcgttact ccctgcgtgt gcgcaacaac
    57781 gaattcaacc acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag
    57841 ttttatgtcg tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac
    57901 ctgaaaccgg tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt
    57961 atgctggtgc gtgagcactt ccctgaaatg ccgatccatc tctcggtaca ggctaacgcc
    58021 gtgaactggg cgacggtgaa attctggcag caaatgggcc tgacccgcgt gatcctctct
    58081 cgcgagctgt cgctggaaga gattgaagag atccgcaatc aggtgccgga tatggagatc
    58141 gagatcttcg ttcacggtgc gctgtgcatg gcctactccg gtcgctgcct gctctctggc
    58201 tatatcaaca agcgcgaccc gaaccagggt acctgcacca acgcctgccg ctgggagtac
    58261 aacgtccagg aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccaatt
    58321 ccggtgcaaa atgttgagcc gacgctgggt atcggcgcgc caaccgacaa agtgtttatg
    58381 atcgaagaag cccagcgtcc gggcgagtat atgaccacgt ttgaagatga gcacggcact
    58441 tacatcatga actcgaaaga tctgcgcgcc atcgcccatg ttgaacgcct gaccaaaatg
    58501 ggcgtgcatt cgctgaaaat cgaaggccgt accaaatctt tctactattg cgcacgcacc
    58561 gcgcaggttt accgtaaagc tatcgatgac gccgctgcgg gcaaaccgtt cgataccagc
    58621 ctgctggaaa cgctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt
    58681 catacccacg acgattatca gaactacgaa tacggttatt cggtttctga ccgccagcag
    58741 tttgttggtg agtttaccgg tgagcgcaag ggggagctcg cggcggtagc ggtgaaaaat
    58801 aaattctccg ttggcgacag ccttgagcta atgacgccgc aaggcaacat taactttacc
    58861 cttgagcata tggaaaacgc caaaggtgaa gcaatgccgg tcgcaccagg cgatggttat
    58921 actgtgtggc tcccggtgcc gcaggatctc gaactgaatt acgcgctgct gatgcgtaat
    58981 ttctccggtg aaaccacgcg taacccccac ggtaagtgat taatttcgat tatttttccc
    59041 ggatgaaaaa ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt
    59101 ttcgctgaaa aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg
    59161 tgtaatctcc cttacacggg cttatttttt atacataaac catagagacg gattgatccc
    59221 aggtcaccgt cttccattga ccacatcgat agaatctccc ttcatagcac gatgcctttc
    59281 acttattggc atcgtgctcg cacaggttcc ggttacgcac agccagaacg cgcatgtttg
    59341 atgcttacca aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt
    59401 aatcctgcac cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag
    59461 aaatgaactg catcattttt tactatattt tctgcaccag atattctacc cctggctaaa
    59521 gaagcttcgg cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat
    59581 gcaagaccaa atttaccgat acttgtctca tcattatgta cagcacaccc ag
//
LOCUS       DAZKJR010000011        57943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKJR010000011 DAZKJR010000000
VERSION     DAZKJR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.011"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..561)
                     /gene="pphB"
                     /locus_tag="ACHOWS_000695"
     CDS             complement(<1..561)
                     /gene="pphB"
                     /locus_tag="ACHOWS_000695"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFQ0046332.1"
                     /translation="MPSTRYQKINAHHYHHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADYFIFGH"
     gene            complement(667..3228)
                     /gene="mutS"
                     /locus_tag="ACHOWS_000696"
     CDS             complement(667..3228)
                     /gene="mutS"
                     /locus_tag="ACHOWS_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0046333.1"
                     /translation="MRTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3514..3858
                     /gene="ygbA"
                     /locus_tag="ACHOWS_000697"
     CDS             3514..3858
                     /gene="ygbA"
                     /locus_tag="ACHOWS_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFQ0046334.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(3897..4325)
                     /locus_tag="ACHOWS_000698"
     CDS             complement(3897..4325)
                     /locus_tag="ACHOWS_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFQ0046335.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(4370..6448)
                     /gene="flhA"
                     /locus_tag="ACHOWS_000699"
     CDS             complement(4370..6448)
                     /gene="flhA"
                     /locus_tag="ACHOWS_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFQ0046336.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRHDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(6522..7532)
                     /gene="hypE"
                     /locus_tag="ACHOWS_000700"
     CDS             complement(6522..7532)
                     /gene="hypE"
                     /locus_tag="ACHOWS_000700"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFQ0046337.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(7529..8650)
                     /gene="hypD"
                     /locus_tag="ACHOWS_000701"
     CDS             complement(7529..8650)
                     /gene="hypD"
                     /locus_tag="ACHOWS_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFQ0046338.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(8650..8922)
                     /gene="hypC"
                     /locus_tag="ACHOWS_000702"
     CDS             complement(8650..8922)
                     /gene="hypC"
                     /locus_tag="ACHOWS_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFQ0046339.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8913..9785)
                     /gene="hypB"
                     /locus_tag="ACHOWS_000703"
     CDS             complement(8913..9785)
                     /gene="hypB"
                     /locus_tag="ACHOWS_000703"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFQ0046340.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9789..10139)
                     /gene="hypA"
                     /locus_tag="ACHOWS_000704"
     CDS             complement(9789..10139)
                     /gene="hypA"
                     /locus_tag="ACHOWS_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFQ0046341.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            10351..10812
                     /gene="hycA"
                     /locus_tag="ACHOWS_000705"
     CDS             10351..10812
                     /gene="hycA"
                     /locus_tag="ACHOWS_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFQ0046342.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10937..11548
                     /gene="hycB"
                     /locus_tag="ACHOWS_000706"
     CDS             10937..11548
                     /gene="hycB"
                     /locus_tag="ACHOWS_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFQ0046343.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            11545..13371
                     /gene="hycC"
                     /locus_tag="ACHOWS_000707"
     CDS             11545..13371
                     /gene="hycC"
                     /locus_tag="ACHOWS_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFQ0046344.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            13374..14297
                     /gene="hycD"
                     /locus_tag="ACHOWS_000708"
     CDS             13374..14297
                     /gene="hycD"
                     /locus_tag="ACHOWS_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFQ0046345.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            14315..16024
                     /gene="hycE"
                     /locus_tag="ACHOWS_000709"
     CDS             14315..16024
                     /gene="hycE"
                     /locus_tag="ACHOWS_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFQ0046346.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            16034..16576
                     /gene="hycF"
                     /locus_tag="ACHOWS_000710"
     CDS             16034..16576
                     /gene="hycF"
                     /locus_tag="ACHOWS_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFQ0046347.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            16576..17343
                     /gene="hycG"
                     /locus_tag="ACHOWS_000711"
     CDS             16576..17343
                     /gene="hycG"
                     /locus_tag="ACHOWS_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFQ0046348.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            17340..17750
                     /gene="hycH"
                     /locus_tag="ACHOWS_000712"
     CDS             17340..17750
                     /gene="hycH"
                     /locus_tag="ACHOWS_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFQ0046349.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17743..18213
                     /gene="hycI"
                     /locus_tag="ACHOWS_000713"
     CDS             17743..18213
                     /gene="hycI"
                     /locus_tag="ACHOWS_000713"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFQ0046350.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(18371..19795)
                     /gene="ascB"
                     /locus_tag="ACHOWS_000714"
     CDS             complement(18371..19795)
                     /gene="ascB"
                     /locus_tag="ACHOWS_000714"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFQ0046351.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(19804..21261)
                     /gene="ascF"
                     /locus_tag="ACHOWS_000715"
     CDS             complement(19804..21261)
                     /gene="ascF"
                     /locus_tag="ACHOWS_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFQ0046352.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            21521..22531
                     /gene="ascG"
                     /locus_tag="ACHOWS_000716"
     CDS             21521..22531
                     /gene="ascG"
                     /locus_tag="ACHOWS_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFQ0046353.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            22680..23207
                     /gene="hydN"
                     /locus_tag="ACHOWS_000717"
     CDS             22680..23207
                     /gene="hydN"
                     /locus_tag="ACHOWS_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFQ0046354.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            23360..25612
                     /gene="hypF"
                     /locus_tag="ACHOWS_000718"
     CDS             23360..25612
                     /gene="hypF"
                     /locus_tag="ACHOWS_000718"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFQ0046355.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAGLGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(25836..26969)
                     /gene="norW"
                     /locus_tag="ACHOWS_000719"
     CDS             complement(25836..26969)
                     /gene="norW"
                     /locus_tag="ACHOWS_000719"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFQ0046356.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQRQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRNARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILAALMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(26966..28405)
                     /gene="norV"
                     /locus_tag="ACHOWS_000720"
     CDS             complement(26966..28405)
                     /gene="norV"
                     /locus_tag="ACHOWS_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFQ0046357.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLTDIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            28592..30106
                     /gene="norR"
                     /locus_tag="ACHOWS_000721"
     CDS             28592..30106
                     /gene="norR"
                     /locus_tag="ACHOWS_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFQ0046358.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     IVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            complement(30103..31068)
                     /gene="gutQ"
                     /locus_tag="ACHOWS_000722"
     CDS             complement(30103..31068)
                     /gene="gutQ"
                     /locus_tag="ACHOWS_000722"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFQ0046359.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31061..31834)
                     /gene="srlR"
                     /locus_tag="ACHOWS_000723"
                     /gene_synonym="gutR"
     CDS             complement(31061..31834)
                     /gene="srlR"
                     /locus_tag="ACHOWS_000723"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFQ0046360.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            complement(31901..32260)
                     /gene="gutM"
                     /locus_tag="ACHOWS_000724"
     CDS             complement(31901..32260)
                     /gene="gutM"
                     /locus_tag="ACHOWS_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0046361.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(32366..33145)
                     /gene="srlD"
                     /locus_tag="ACHOWS_000725"
     CDS             complement(32366..33145)
                     /gene="srlD"
                     /locus_tag="ACHOWS_000725"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFQ0046362.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            complement(33149..33520)
                     /gene="srlB"
                     /locus_tag="ACHOWS_000726"
                     /gene_synonym="gutB"
     CDS             complement(33149..33520)
                     /gene="srlB"
                     /locus_tag="ACHOWS_000726"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0046363.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(33531..34490)
                     /gene="srlE"
                     /locus_tag="ACHOWS_000727"
                     /gene_synonym="gutE"
     CDS             complement(33531..34490)
                     /gene="srlE"
                     /locus_tag="ACHOWS_000727"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0046364.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(34487..35050)
                     /gene="srlA"
                     /locus_tag="ACHOWS_000728"
                     /gene_synonym="gutA"
     CDS             complement(34487..35050)
                     /gene="srlA"
                     /locus_tag="ACHOWS_000728"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0046365.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            35306..36391
                     /gene="mltB"
                     /locus_tag="ACHOWS_000729"
     CDS             35306..36391
                     /gene="mltB"
                     /locus_tag="ACHOWS_000729"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFQ0046366.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            36536..37033
                     /gene="pncC"
                     /locus_tag="ACHOWS_000730"
     CDS             36536..37033
                     /gene="pncC"
                     /locus_tag="ACHOWS_000730"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFQ0046367.1"
                     /translation="MTDSELMQLSEQVGQSLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            37113..38174
                     /gene="recA"
                     /locus_tag="ACHOWS_000731"
     CDS             37113..38174
                     /gene="recA"
                     /locus_tag="ACHOWS_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0046368.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            38243..38743
                     /gene="recX"
                     /locus_tag="ACHOWS_000732"
     CDS             38243..38743
                     /gene="recX"
                     /locus_tag="ACHOWS_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0046369.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            38871..41501
                     /gene="alaS"
                     /locus_tag="ACHOWS_000733"
     CDS             38871..41501
                     /gene="alaS"
                     /locus_tag="ACHOWS_000733"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0046370.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVNSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            41736..41921
                     /gene="csrA"
                     /locus_tag="ACHOWS_000734"
     CDS             41736..41921
                     /gene="csrA"
                     /locus_tag="ACHOWS_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFQ0046371.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            42237..42329
                     /locus_tag="ACHOWS_000735"
     tRNA            42237..42329
                     /locus_tag="ACHOWS_000735"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42271..42273,aa:Ser,seq:gct)
     gene            42333..42409
                     /locus_tag="ACHOWS_000736"
     tRNA            42333..42409
                     /locus_tag="ACHOWS_000736"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42367..42369,aa:Arg,seq:acg)
     gene            42608..42684
                     /locus_tag="ACHOWS_000737"
     tRNA            42608..42684
                     /locus_tag="ACHOWS_000737"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42642..42644,aa:Arg,seq:acg)
     gene            42747..42823
                     /locus_tag="ACHOWS_000738"
     tRNA            42747..42823
                     /locus_tag="ACHOWS_000738"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42781..42783,aa:Arg,seq:acg)
     gene            42887..42963
                     /locus_tag="ACHOWS_000739"
     tRNA            42887..42963
                     /locus_tag="ACHOWS_000739"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42921..42923,aa:Arg,seq:acg)
     gene            43244..43810
                     /gene="yqaB"
                     /locus_tag="ACHOWS_000740"
     CDS             43244..43810
                     /gene="yqaB"
                     /locus_tag="ACHOWS_000740"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFQ0046372.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            43807..44235
                     /gene="yqaA"
                     /locus_tag="ACHOWS_000741"
     CDS             43807..44235
                     /gene="yqaA"
                     /locus_tag="ACHOWS_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFQ0046373.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            44308..45864
                     /gene="gshA"
                     /locus_tag="ACHOWS_000742"
     CDS             44308..45864
                     /gene="gshA"
                     /locus_tag="ACHOWS_000742"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFQ0046374.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFIAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            46014..46529
                     /gene="luxS"
                     /locus_tag="ACHOWS_000743"
     CDS             46014..46529
                     /gene="luxS"
                     /locus_tag="ACHOWS_000743"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0046375.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(46580..47692)
                     /locus_tag="ACHOWS_000744"
     CDS             complement(46580..47692)
                     /locus_tag="ACHOWS_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0046376.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALVTQPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(47689..48396)
                     /locus_tag="ACHOWS_000745"
     CDS             complement(47689..48396)
                     /locus_tag="ACHOWS_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFQ0046377.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINADYWQDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFDYGEQS"
     gene            complement(48654..50192)
                     /gene="emrB"
                     /locus_tag="ACHOWS_000746"
     CDS             complement(48654..50192)
                     /gene="emrB"
                     /locus_tag="ACHOWS_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFQ0046378.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(50209..51381)
                     /gene="emrA"
                     /locus_tag="ACHOWS_000747"
     CDS             complement(50209..51381)
                     /gene="emrA"
                     /locus_tag="ACHOWS_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFQ0046379.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(51508..52038)
                     /gene="emrR"
                     /locus_tag="ACHOWS_000748"
     CDS             complement(51508..52038)
                     /gene="emrR"
                     /locus_tag="ACHOWS_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFQ0046380.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(52129..52464)
                     /gene="ygaH"
                     /locus_tag="ACHOWS_000749"
     CDS             complement(52129..52464)
                     /gene="ygaH"
                     /locus_tag="ACHOWS_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFQ0046381.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(52454..53191)
                     /gene="ygaZ"
                     /locus_tag="ACHOWS_000750"
     CDS             complement(52454..53191)
                     /gene="ygaZ"
                     /locus_tag="ACHOWS_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFQ0046382.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(53315..54499)
                     /locus_tag="ACHOWS_000751"
     CDS             complement(53315..54499)
                     /locus_tag="ACHOWS_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0046383.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRTL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLPWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASVWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AI"
     gene            complement(54691..55683)
                     /gene="proX"
                     /locus_tag="ACHOWS_000752"
     CDS             complement(54691..55683)
                     /gene="proX"
                     /locus_tag="ACHOWS_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFQ0046384.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(55741..56805)
                     /gene="proW"
                     /locus_tag="ACHOWS_000753"
     CDS             complement(55741..56805)
                     /gene="proW"
                     /locus_tag="ACHOWS_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFQ0046385.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(56798..>57943)
                     /gene="proV"
                     /locus_tag="ACHOWS_000754"
     CDS             complement(56798..>57943)
                     /gene="proV"
                     /locus_tag="ACHOWS_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFQ0046386.1"
                     /translation="RAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLS
                     GSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHM
                     TVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARAL
                     AINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAI
                     MQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFG
                     PRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQ
                     TPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT        13438 a        15110 c        15789 g        13606 t
ORIGIN      
        1 atgtccaaat ataaaataat cagcgccgtt tattttttgt aattcgctat taagtgattt
       61 ctgcacacga tcaacaggcc agagtaattc gctctccgct atttctttgc caaactgata
      121 ctccttcccc ggataatctg catgtgcgat gacatatttt atggtgtcgt tagtgatttc
      181 aataatatgt ggaaggtgat ggaatttcag caacagatct attgcctctt gttgctctga
      241 atcatttaaa tcgaaaaacc agtcaccacc gctggcaagc cacatatttc catcgccagt
      301 ttcgaatgcc tcaagcgcca tcgcttcgtg gttgcctttg acggagataa accagggctg
      361 gtttaacagg cgcagcacgt taagactctc gggcccacga tcgatgttat cgccgacaga
      421 aataagcaag tcggtttcag gacaaaaaga gagttgatgt aagcgggatt gtaataattg
      481 atagtcacca tgaatatcac caacggccca tatatggtga tagtgatggg cattgatttt
      541 ttggtagcgt gtagatggca tggttttacc ctgtaaaata agatttccta ttatacaggg
      601 tatttttatt tgattcgtca gttgtcgtta atattcccga tagcaaaaga ctatcgggaa
      661 ttgttattac accagactct tcaagcgata aatccactcc agcgcctgac gcggagtcag
      721 tgaatccggg tcgagatttt ccagtgcttc gaccgcaggc gacgtttctt ctggcactga
      781 cagcaaagac atttgcgtac catccacttg cgtagcggcg gcgttcggcg aaatgctttc
      841 cagctcacgc agtttttgcc gtgcgcgctt aataacctct tttggcacgc cggccagagc
      901 tgcaaccgcc aggccgtagc ttttgctcgc tgcgccatcc tgcacgctgt gcataaaggc
      961 aatggtgtcg ccgtgctcca gtgcatcgag atgcacgttg gcgacgcctt ccattttctc
     1021 cggtaactgg gtcagctcga aatagtgggt agcaaacaac gtcaacgctt taatcttatt
     1081 cgccagattt tccgcgcacg cccacgccag cgacagacca tcgtaagtgg acgttccacg
     1141 cccaatctca tccatcagca ccagactgta ctcggtggcg ttatgtaaaa tattggcggt
     1201 ttcggtcatt tccaccataa aggttgaacg cccggatgcc agatcatccg ctgcccctac
     1261 gcgggtaaag atgcgatcga taggtccaat ctcgactttt tgtgccggta catagctgcc
     1321 gatgtaggcc atcagcgcaa tcagtgcggt ctggcgcata taggtacttt taccgcccat
     1381 attcggaccg gtaatgatca acatgcgacg ctgcggcgac aggttcagcg ggttggcgat
     1441 aaacggctca ttcagcacct gttcaaccac cggatggcgg ccttcggtga tgcgaatacc
     1501 cggtttatca ataaaggtcg ggcaggtgta gttcagcgta taggcccgtt ccgccaggtt
     1561 caccagcacg tcgagttccg ccagcgcgct cgcgctctgt tgcaacgctt ccagatgcgg
     1621 caacagcagg tcgaacaact cttcataaag ctgtttttcc agtgccagtg ctttgccttt
     1681 tgaagtgaga actttgtctt cgtactcttt tagctctgga ataatgtagc gctcggcgtt
     1741 tttcagcgtc tggcgacgca tgtaattgat gggtgccaga tggctttgtc cacggctgat
     1801 ctgaatgtaa taaccatgaa ccgcgttaaa gccgactttc agggtgtcca ggccagtacg
     1861 ttcacgctcg cggacttcca gacgctccag ataatcggtc gcgccgtcag ccagcgcgcg
     1921 ccattcatcc agttcttcgt tataaccagt cgcaataaca ccaccgtcgc gtaccagcac
     1981 cggcggcgtg tcgatgattg ctcgctccag caggtcgcgc agttcggcaa actcgcccat
     2041 cttctcacgc agtgcttgta ccggggcgct atcgacacct tccagttgcg cgcgcagctc
     2101 cggcagttgc tggaaagcgt gacgcatacg ggccagatcg cgtggacgag cagttcgtaa
     2161 agccagacgc gccagaatac gttccaggtc gccgacctga cgcagtaccg gctgcagctc
     2221 ggcggtgaaa tcctgcaatg cgccaatagt ttgctggcgc tcaagcaaca cgcgggtatc
     2281 gcgcactggc atatgcagcc agcgtttcag catacgacta cccatcggcg ttacagtaca
     2341 gtcgagcact gaagccagcg tattttccgc accgccggcc aggttctgag taatttccag
     2401 gttacgacgc gtcgcggcat ccataatgat gctgtcctgc tgacgttcca tagtgataga
     2461 acgaatatgc ggcagggtcg tgcgttgggt atctttcgca tactgcaaca gacaaccggc
     2521 agcacaaagt ccgcgtggtg cgttctccac accaaaaccg accagatctc gggtgccaaa
     2581 ttgcaggttc aattgctggc gagcggtgtc gatttcaaac tcccacagcg ggcgacgacg
     2641 caggccgcgg cgaccttcaa tcagcgacat ctcggcgaaa tcttccgcat acagtaattc
     2701 cgctggattc gtacgttgca gttctgccgc catcgtttcg cggtccgccg gttcgctcag
     2761 gcgaaaacgc ccggagctga tatccagcgt ggcgtagccg aaccccttgc tgtcctgcca
     2821 gatagccgcc agcaggttgt cctgacgttc ctgcaacagc gcttcatcgc tgatggtgcc
     2881 cggcgtaacg atacggacaa ctttgcgctc gaccggccct ttgctggtcg ccggatcgcc
     2941 aatctgttca caaatagcaa ccgattcgcc ctgattcacc agtttggcga ggtagttttc
     3001 caccgcatgg tagggaatcc ccgccatcgg gattggctct cccgctgaag caccgcgttt
     3061 ggtcagtgaa atatccagca gttgcgacgc ccgttttgcg tcgtcataaa acagttcata
     3121 aaagtcgccc atccggtaaa acagcaggat ctcgggatgc tgggctttca gcttgagata
     3181 ctgctgcatc ataggcgtat gggcgtcgaa attttctatt gttctcatgg ggttaagtcc
     3241 ggctccctga tgttaaatgt agtgtgatgg tttttatttt cgttgtaatc acataaggcg
     3301 cacatgatac cggagttaat catttcaggg gaagacgcga aaggtgagtt cttgatgtcg
     3361 gttccgcgaa aaatgctgct ttgcagcttt tgcgtgatga cgatgaaaca agatcgccaa
     3421 attcgtcggt tgaagaaacc tcctccttca gagccatagt cccctttaaa attaagttgt
     3481 aatataaatg caccttaaga gtatggaggc agcatgtccg gcaagcgtat ctctcgtgaa
     3541 aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca ggcgtcagca
     3601 gagccggagc attacgaggc gttgtttgct tacgcgcaaa agcggctgga taaatgtgtg
     3661 ttcggtgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca gcctgcaaag
     3721 cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg gcgtcatccg
     3781 atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccggagtt gccagaaaaa
     3841 tatcgtccaa agaaataaat ctgccggaga gccatactct ccggcaaata aggcgtttac
     3901 gctttggtgg caatcaggat ggctgatgct ttgatcaatg caataacgcg cgagccagtg
     3961 gtgagtttca tttcatcctg actttcattt gttaccactg ccacaatttc aaaacccgca
     4021 tccgttttga tatggacagt ggcgttcact gcgccttcgg tgattgtcga cacgctgcca
     4081 gcaaactggt tgcgagcaga gaatttcagt ccacagtctt ccgttgccag cgtgacccac
     4141 ggggctttaa tcagggcaat cgcttctttc cctttcgcca gacccagcgc ttgctggctg
     4201 ctgtgagtca caattgccac cagctttgca ccacctgcca gcgtcagttc tacttcatca
     4261 ttcacagcgc ccattgctac tgtgctaact gttccggtta attgatttct tgctgaaact
     4321 gccatactgc ctcctttcat tgaatgttga tcgagtataa ccacatctgt taaatcaatg
     4381 ccgatttatc aattcccagt cgcttcatcc gtgacagcag agtcgtgcgt ttcagcccca
     4441 gacgttgcgc agcgccttta ggcccggcaa ccacgccgtt tgtttctttc agcacgcgaa
     4501 caatcaactg atattcatct tcgccctcct gggcgacaac cgttgcggca ggcggcgttt
     4561 caggttctgg taaagcaata tctggcaatg acagctgcag cacgttgccg cgtgttagca
     4621 ataccgcgcg ctcaatgacg ttttccagct cacgcacgtt gcccggccat tccatgttgc
     4681 tcaggatgcg cagcgtctcg gcaggaatgc tgtcgatatt gcgccccaga cgacgggcaa
     4741 ttttgaaggt aaaggctttc gccagcagcg gaatatcttc cggacgctcg cgtagtggcg
     4801 gcaggtggat cgggaatacg ttcagacggt aatagagatc gctacggaac tctcggtcgg
     4861 cgaccatttt tttcagatcg cggttagtcg cggcaattaa acgtacgtcc gtctgaatga
     4921 ttttgttgct gccgagacgt tcaaactcct gttcctgtaa tacacgcagc aacttcggct
     4981 gtaactccag cggcatatcg cccacttcat cgaggaatag tgagctttta tccgccagtt
     5041 caaaacgacc aatgcgctga gcgctggcac cggtaaaagc cccacgctca tgaccaaata
     5101 gatcgctttc cagcaatccg gcaggcatcg ccgcgcagtt cattttgacc atacggcgat
     5161 tattacgccc actgagatta tggatcgcgc gggcaatcag ctctttacct gtaccagttt
     5221 caccgaggat cagaacggta ctgtcacttt gcgccaccat ttccacttgt ttaagtacgc
     5281 tgtacatggc ttcgctgcgg ccaataatct cgccaaattc actatcaaca ttgttgagct
     5341 gctcggtcag ggcgaggttt tcatcaacca gccgttcttt cagacgatga atttcctgat
     5401 aggcgagggc gttatcgaca gcgattgcca cacgttcggc aatctggcgc agtaagctca
     5461 gattggtagt ggtaaagact ttctcttcgc attgcgccag ttttagcacg cccagcatgg
     5521 tgtcgccaga catcagcggt aacaggcaca aggtttgaat ctggttgccc caggtgttga
     5581 acaacatgcg ttcatagggg gctaaatcgt cccgctcgtg gagattaatc aacagcatct
     5641 ctttactttt gaagacgcgt tcggtgaggg ttccggcttc atcgacttcg ctctgttcgt
     5701 gagcgggatg ctgtttatca agatagtgag tggagtagat gttgagtttg tttttacggt
     5761 ggctgcgtaa gacgatgctg atatcgtcaa tgtcgaagta gtagtggatt tctttggcga
     5821 cttcgctgac cagttcgtcc atatccaggc gggaaagcac cgcgttagtg atggcgacca
     5881 ggatgcggaa gttatcgcgt tcccggcata acaactcata gtcgacattg ttaacgacgc
     5941 ggctctggat ttgttcggtg acgacagaaa cgatctgcgt aaatgtttgc agacgattga
     6001 actctttttc gctccagggg cgatcgtcat gacgaataaa ttcacagcca ccaaaaatat
     6061 gcccttccgc cgccagtggc atcaggcaat agtgaccaaa tttggggtat agcccacctg
     6121 tggccagctg cggccaggtt tcacaaaatt cttcgtaact gcaatgcagc gtatcagggc
     6181 gcgacaaaat gctgcgtacc ggaccgtgtg ccagaacagt ttcgtcttca tatttaatgg
     6241 gggtgtcttt ttcacgcgat gcgtaataag acgcacgttg agtctgcgct tgccacaaca
     6301 caatagccgc gttgtcggcg agcgcagaac gctttaccag ttgcgaaaga gcctcacaca
     6361 gcgaggccag atcgggctgc tgcaatagtg tccgagtgat gtcgaacaac ccttgctgtc
     6421 cgagatcact catcggtgta tatgacattt tgttcttcca ggaaagcacc gcaaagcggt
     6481 gcaaaaggtc attgactaac tataggagat ttagaatttt attagcatat acgcggaagc
     6541 ggttcggcgt gcggtagatc gagggttcgt ttcacgccat acagaccggc aagacgaaca
     6601 cctttacgtt ctactacttc gccaatcagc gccgcatctt ttcccaatgg atgggaatgt
     6661 aacgccgcca gcacttgctc tgctgcgttg cgttcaacgg cgatcaccag tttgccttcg
     6721 ttggcaaagt taagggcgtc cagtcccagc agttcgcaaa cgccgcgcac agcaggttta
     6781 accggcagtg ccgcttctga aagttcaata ccacaaccgc aggctgccgc gaactcatga
     6841 accaccgcgt ttacaccacc acgggtggca tcacgcagcg ctttcacgcc gggaatgtca
     6901 cgcagcgtct gaataagcgg cgtcagcacc gcgcagtcgc tgaccagttc tccatccagc
     6961 cctagttgct cacgcaggtt aaggatagtc gccccatggt cgccgagtgt accgctaacc
     7021 agcaatacat cgcctgcggt tagcgtctgt gcgccccagt gaatattcgc cggaattgcg
     7081 cccatgccag cggtgttgat aaacagttta tctgccgcgc cgcgctgtac cactttggta
     7141 tcgccagtaa cgatggcaat gcctgccgcg cgggcggttt ctgccatgct ggtcactacg
     7201 gctttcagtg tctccatcgg caatccttct tcgaggataa agccacagga gagatagcgc
     7261 ggaattgcgc cactgaccgc aacgtcattg gcggtgccgc aaatcgccag tttgccgata
     7321 ttaccgccag gaaagaacag cgggtcaatg acgtaactgt cggttgagaa cgccagacgg
     7381 tcgccttccg ctaccagctg tgccaaatca agacgggcct gatcttcctg ctctgccagc
     7441 cacgggttgg caaaggcttc cataaacagg ctgttgatta attgctgcat cgcctggccg
     7501 ccgctaccgt gggcgagttg gatattattc acgcttcact ctcctgctga cgatactgat
     7561 accacgcggc gcacgctcct tcggaggaaa ccatcagcgc accaaacgcg gtttgaggat
     7621 tacaggtgtt accaaacagc gggcattgat gcggcttaca tttgcccgtc aagacttcgc
     7681 cacaacgcgc acgcgggtca tcgcagacct gctgcggtgc cgggcggaaa tgtgtttcgg
     7741 catcgaatcg ttgataatcc ggcgtcaggt gcacgccaga agattcaatc acgcctaagc
     7801 cgcgccattc actgtcgccg ttgacacaga acacatcggc aatcgcctgt tgcgccagca
     7861 ggttaccggc atccggtacc acccgacgat actgattctc taccttgctg tgggccgcta
     7921 ttttctgctc caccagcatc accacgcctt gcagtagatc aaggggttcg aaaccagcga
     7981 ccaccagcgg gcgatgaaaa tcgctggcga taaaattata ggcatcagtg ccgataacca
     8041 tactgacgtg gcccggcgcg aggaacgcgt cgataccgtt atctggctgt tccagcaaac
     8101 tgcgcagcgt cgggataagc gtaatgtgct ggcagaagaa gtaaaaattc tgcacatcgc
     8161 gggctttcgc ctgttgcaga gtgatggcgg tggtcggcat ggtggtttca aaacctaagc
     8221 cgaagaatac cactttacgg gttggattct cctgcgccag ttttaacgca tccattggcg
     8281 agtaaacgat gcgcacatcg gcaccgcgtg cttttgcctg caacagcgac ccctgtttcc
     8341 ctggtacgcg catggcatcg ccaaaggtac agaagatgac ttccggatgg ctggcaatct
     8401 ccacgcaggt gtcgattcta cccattggca gtacgcacac cgggcaaccc ggaccgtgga
     8461 taaactcaac gttttccggt agcaactggt cgaggccgaa tttaaaaatg gcgtgggtat
     8521 gaccgccaca cacttccata atccgcagag ggcgttcggc ggtgtaagag agatgtgaag
     8581 cacgttcgcg cagatgctca attaactgca tcacctgttc cggcgcgcga tattcatcaa
     8641 caaaacgcat tatttttcct cgccatacaa cagcgcgccg acatctggct caacgtcaaa
     8701 catgttttgt aaggcgtcga gagtgtcgcg tgcttcggct tcattaatta cgctcatggc
     8761 aaagccaacg tgaaccagta cccactggcc cacgcgtggc tgaccgtttt catcgcagct
     8821 gccgactaac gttaaatcga catcgcgctg aatgccgcag acgtcgactt tcgcctggtt
     8881 gccgtcaata gtgcggatct ggccgggaac gcctatgcac atcgctgtgt ctccagccag
     8941 ttcagccact ggtccatccc ttcgccgctg gtggcggaaa taaggatgat ttcaatttct
     9001 ggattgactt cacgggcgca ggcgatgcac ttctcaacgt cgaaattgag atacggcagc
     9061 aagtcaactt tgttgagcag catcagcgag gcggcggcaa acatgtgcgg atatttcagc
     9121 ggtttgtctt caccttcggt aacggaaagc accgccactt tgtgtttttc accgagatcg
     9181 aagctggccg ggcaaacgag gttaccgacg ttttcgataa acagaatacc gttatcgtcc
     9241 agtggcaggc gcggcgcggc gtcggcaatc atctgtgcgt caagatggca gcctttaccg
     9301 gtgttcacct gaatcgctgg ggtgccggta gcgcgaatgc gtgcggcatc gttcacggtt
     9361 tgctggtcac cttcaataac cgcgcacgga acgctgtctt tcaggcgcat taaagtttcc
     9421 gtcagcaggg tggttttacc ggaaccaggg ctggaaacca ggttgagcac cagttgcttg
     9481 cgggcagcaa agcgcgcgcg gttgcgttca gccagacggt tatttttgtc cagcacgtca
     9541 atttcgactt ccagcatccg acgctggctc atgcccggcg cgtgagtgcc cgcttcgcca
     9601 tgaccgtaat gcaggtcgcc ttcttcagtc tggctggggg taaattcagg cgctttaatg
     9661 ccggtgattt tcatcttcgg gcgtgccgcc ggggcaaatg gcgcgctacg aaacgcggaa
     9721 tgagggttat gttcatcacc ctcaatatag aggttgcctt caccgcaacc gcatgttgta
     9781 cacatcgctc actcctggtc tatttctatc cgccgaatct gtaaaccgtc gtctgccaca
     9841 atctgtagca tgtcaccatg acactgtgga cagcggcgga cgcgctgggt cagtagcgtc
     9901 acatactgtt ggcatgtttc acaccagcat tcggcctctt gttcttcgag gtgcagttta
     9961 caaccttccg ccacgctgcc gcggcaaacc agatcaaaac aaaaggcaag agagctggtt
    10021 tcgacacaag aaaatgcgcc aattttgagc cagaccccag ttacgcgttt tgcgccgtgt
    10081 tttgcggcct gctgttcgat caattccagt gcccgttggc agagggttat ttcgtgcata
    10141 tcgcctccca ttaactattg ccagctacaa gcaataattg tgccagtgtt gattatccct
    10201 gcggtgaata atgtcgatga tgtcgaaatg acacgtcgac acggcgacga aattcatctt
    10261 tagcttaaaa atcgctttaa taacaataaa ttaaaagttg gcacaaaaaa tgcttaaagc
    10321 tggcatctct gttaaacggg taacctgaca atgactattt gggaaataag cgagaaagcc
    10381 gattacatcg cacagcggca tcgtcgccta caggaccagt ggcacatcta ctgcaattcg
    10441 ctggttcagg gaatcacgtt atcgaaagcg cgcctgcatc acgccatgag ctgcgcgccg
    10501 gacaaagaac tctgtttcgt cctttttgaa cattttcgta tttacgtcac cctggcggat
    10561 ggctttaaca gccacaccat cgagtattac gtcgaaacaa aagatggtga agacaaacag
    10621 cggattgcgc aggcgcaact gagcattgac ggcatgattg atggcaaggt caacatccgc
    10681 gatcgcgaac aggttctgga acactatctc gaaaaaatcg ctggcgttta cgacagctta
    10741 tacaccgcca ttgaaaacaa tgtgccggtg aatttaagcc aactggtaaa gggacaaagc
    10801 ccggcagcat gagctgaggc tttgcccgtt ttgcaggcgt tacgcctgtt tggggatggg
    10861 cgtgtcgatg agtgtcgaaa atgacatttc atcggcatgt tttcgtcaaa aatgacaatc
    10921 acctgaggaa tgcctggtga atcgttttgt aattgctgac tccacgctct gtatcggctg
    10981 ccacacctgt gaggccgcct gttcagagac gcatcgccag cacggcctgc aatcaatgcc
    11041 gcgcctgcga gtgatgctca atgaaaaaga atctgcgccg cagctctgtc accactgtga
    11101 agatgcgccc tgtgcggtgg tttgcccggt taacgccatc acccgcgttg atggggccgt
    11161 gcagttgaat gaaagtctgt gcgtaagctg caaactgtgc ggcatcgcct gcccgtttgg
    11221 cgcaattgaa ttttccggca gccgtccgct ggatattccg gcaaacgcca ataccccgaa
    11281 agcgccaccg gcaccgcctg ctccggcgcg tgtcagcaca ttgcttgact gggtgccagg
    11341 tattcgcgcg atcgccgtca aatgtgacct ttgtagcttt gatgaacaag gtccggcctg
    11401 cgtgcggatg tgcccgacta aagccctgca tctggtggat aacaccgata tcgcccgcgt
    11461 cagcaaacgt aagcgtgagc tgacttttaa cacggatttt ggcgatctca ccttgtttca
    11521 gcaggctcaa agtggagagg ctaaatgagc gcaatttccc tgatcaatag cggcgtggcg
    11581 tggtttgtcg ccgccgctgt tctggcattt ctcttttctt ttcaaaaggc gctaagcggc
    11641 tggatagccg gaattggcgg cgcggttggt agcctgtata cggcagccgc gggcttcaca
    11701 gtgctgactg gcgcggttgg cgtgagcggt gcgctgtcgc tggtaagcta cgatgtgcaa
    11761 atctctccgc ttaatgcgat ttggctgatt acgctcggcc tatgcggtct gtttgtcagc
    11821 ctctacaaca ttgactggca tcgccacgcg caggtgaagt gcaacggctt gcagatcaat
    11881 atgttgatgg ctgccgccgt ctgcgccgtc atcgccagca acctcggcat gttcgtggta
    11941 atggccgaaa tcatggccct gtgtgcggtg ttcctcacca gcaacagcaa agagggcaaa
    12001 ctgtggtttg cgctggggcg tcttggcact ctgctgctgg cgattgcctg ctggctgctg
    12061 tggcagcgtt acggcacgct ggatctgcgc ctgctggata tgcgtatgca acagctgccg
    12121 ctcggttccg atatctggct gctcggtgtg attggctttg gcctgctggc cgggattatt
    12181 ccgctgcacg gctgggtgcc acaggcacat gccaacgcct ctgcgccagc tgccgcgctg
    12241 ttttccacgg tagtcatgaa aattggcctg ctgggcattt taactctgtc actactgggc
    12301 ggtaatgcac cgctgtggtg ggggatcgca ctgctggtac tcggcatgat caccgcattc
    12361 gtcggcggtc tgtatgcgtt gatggaacat aatatccaac gtctgctggc ttaccacacc
    12421 ctggaaaata tcggcatcat cctgctgggg ctgggcgctg gcgtaacggg tatcgcgctc
    12481 gaacaaccgg cgctgattgc ccttggtctg gttggtggtc tgtaccatct gcttaaccat
    12541 agcctgttca agagcgtact gttccttggt gcggggagcg tctggttccg taccggtcat
    12601 cgcgatatcg aaaaactcgg tggtattggc aagaaaatgc cggttatctc catcgccatg
    12661 ttagtcgggc tgatggcaat ggctgcgctg ccgccgctga acgggtttgc cggggaatgg
    12721 gttatctatc aatccttctt caaactgagc aatagtggcg cgtttgttgc ccgtcttctc
    12781 gggccgctgc tcgccgtggg gctggcaatt accggtgcgc tggcggtgat gtgtatggcg
    12841 aaagtttatg gcgttacttt cctcggcgcg ccgcgtacca aagaggccga aaacgccacc
    12901 tgtgcgccgc tcctgatgag cgtaagcgta gtggcactgg cgatttgctg cgtaattggc
    12961 ggtgttgctg cgccgtggct actgccgatg ctctctgctg ctgtacctct gccgctggag
    13021 cctgctaaca ccaccgtttc tcaacccatg atcacgttgc tgctgattgc ctgcccgctg
    13081 ctgccattca tcattatggc gatttgcaaa ggcgatcgtt tgccgtcgcg ttcccgcggc
    13141 gcggcctggg tgtgcggcta cgatcatgaa aaatcaatgg tgattaccgc tcacggtttt
    13201 gccatgccgg tgaaacaggc gtttgcgccg gtgctgaaac tacgcaaatg gctgaatccg
    13261 gtgtcgctgg tgccgggctg gcagtgcgag gggagtgcgt tgctgttccg ccggatggcg
    13321 ctggttgaac tggcggtact ggtggtgatt attgtttcac gaggagcctg agaatgagtg
    13381 ttttatatcc gttaattcag gcgctggtgt tatttgccgt tgcgccgctg ctctccggta
    13441 ttacccgcgt agcgcgcgcc cgcttgcata accgtcgtgg gccgggcgtg ttgcaggagt
    13501 atcgcgacat tatcaaactg ctggggcgtc agagcgttgg cccggatgcc tccggctggg
    13561 tgttccgcct gacgccgtat gtgatggtgg gcgtcatgct gactatcgcc actgcgctgc
    13621 cggtggtgac cgtcggctct ccgctgccgc aactgggcga tttgatcacc ttactgtatc
    13681 tcttcgccat cgcgcgtttc ttctttgcca tttctggtct ggatactggt agcccgttta
    13741 ccgctatcgg cgcgagccgt gaagcgatgc ttggcgtgtt ggttgaaccg atgctgctgc
    13801 ttggtctgtg ggtcgccgca caggttgccg gttccaccaa catcagcaac atcaccgaca
    13861 ccgtttatca ctggccactg agccagagca tcccgctggt gctggcgctt tgtgcctgtg
    13921 cgttcgccac ttttatcgaa atgggcaaac tgccgttcga cctggcggaa gccgagcagg
    13981 agttgcagga aggtccgctc tctgagtata gcggcagcgg ctttggcgtc atgaaatggg
    14041 gcatcagcct gaaacagctg gtggtgttgc agatgttcgt cggggtgttt attccgtggg
    14101 gacaaatgga aaccttcacc gtcggtgggc tgctgctggc gctggtgatt gccatcgtca
    14161 aactggtggt cggcgtactg gttatcgcgc tgttcgaaaa cagcatggcc cgtttgcgtc
    14221 ttgatattac tccacgcatt acctgggctg gttttggctt tgcattttta gcgttcgtct
    14281 ccttgctggc ggcgtgatta aagagagttt gagcatgtct gaagaaaaat taggtcaaca
    14341 ttatctcgcc gcgctgaatg aggcatttcc gggcgtcgtg ctggaccacg cctggcagac
    14401 caaagatcag ctgaccgtga ccgtgaaggt gaactacctg ccggaagtgg tggagtttct
    14461 ctactacaag caggggggct ggctgtcggt gctgtttggt aacgacgaac gcaaactgaa
    14521 tggtcattac gccgtttatt acgtgctgtc gatggagaag gggactaagt gttgggtaac
    14581 ggtgcgcgtc gaagttgacg ccaacaaacc ggagtatcct tccgtgacgc cgcgcgttcc
    14641 ggcggcagtg tggggcgagc gtgaagtgcg cgatatgtac ggtttgattc cggttggtct
    14701 gccggatgaa cgccgtctgg tgctgccgga tgactggccg gatgaacttt atcctctgcg
    14761 taaagacagc atggattatc gtcagcgtcc ggcaccgact accgatgctg aaacctacga
    14821 gttcatcaac gaactgggcg acaagaaaaa caacgtcgtg ccgattggtc ccctgcacgt
    14881 cacttctgac gaaccgggtc acttccgtct gtttgtcgat ggtgaaaaca ttatcgacgc
    14941 cgactaccgc ctgttctacg tccatcgcgg catggaaaaa ctggcggaaa cccgtatggg
    15001 ttacaacgaa gtgaccttcc tctctgaccg tgtgtgcggg atctgcggct ttgctcacag
    15061 caccgcctac accacgtcgg tggaaaacgc gatgggtatt caggtgccag aacgtgcgca
    15121 gatgatccgc gccattctgt tggaggtgga acgcctgcac tcgcatctgc tcaatctcgg
    15181 cctcgcctgt cactttaccg gcttcgactc cggctttatg cagttcttcc gcgtgcgtga
    15241 aacctccatg aaaatggcag agatccttac cggtgcacgt aaaacctacg gcctgaacct
    15301 gatcggcggg attcgtcgcg atctgctgaa agacgacatg atccagaccc gccagctggc
    15361 acaacagatg cgtcgtgaag tgcaggagct ggtggatgtg ctgctgagca caccgaacat
    15421 ggaacagcgc accgtcggca ttggtcgtct ggacccggaa atcgcccgcg atttcagtaa
    15481 cgttggcccg atggttcgcg ccagcggtca cgcccgcgat actcgcgccg atcacccgtt
    15541 tgtcggttat ggcctgctgc caatggaagt ccacagcgag cagggctgcg acgttatttc
    15601 ccgtctgaaa gtgcgaatca acgaggtcta taccgcgctg aacatgatcg actacggtct
    15661 ggataacctg ccgggcggtc cgttgatggt agaaggtttt acctacattc cgcaccgttt
    15721 tgcgctgggc tttgccgaag cgccgcgcgg cgacgatatc cactggagca tgaccggcga
    15781 caaccagaag ctgtaccgct ggcgctgccg tgccgcgacc tacgcgaact ggccgaccct
    15841 gcgctacatg ctgcgcggca acaccgtttc cgatgcgccg ctgattatcg gtagcctgga
    15901 cccttgctac tcctgtaccg accgcatgac cgtggtcgat gtgcgtaaga agaagagcaa
    15961 agtggtgccg tacaaagaac tcgagcgtta cagcattgag cgtaaaaact cgccgctgaa
    16021 ataaggaatc gccatgttta cctttatcaa aaaagtcatc aaaaccggca cggcgacctc
    16081 gtcttatccg ctggagccga ttgcggttga taaaaacttc cgtggtaagc cagagcagaa
    16141 cccgcagcag tgcatcggct gcgcggcctg cgtcaatgcc tgcccgtcaa acgccttaac
    16201 ggttgaaacc gacctcgcca caggagagct ggcctgggag tttaatctcg ggcgctgcat
    16261 cttctgtgga cgctgcgaag aagtttgccc gacggcggcg atcaaactgt cgcaagagta
    16321 cgaactggcg gtgtggaaga aagaagactt cctccaacag tcccgcttcg cgctgtgcaa
    16381 ctgccgcgtc tgcaatcgtc ctttcgccgt ccagaaagag atcgactacg ccattgcgct
    16441 gcttaagcac aatggtgaca gccgcgcgga aaaccaccgc gaaagctttg agacttgccc
    16501 ggaatgtaag cgccagaaat gcctggtgcc gtccgaccgt attgaactga ctcgccatat
    16561 gaaagaggcc atctgatgag caatttatta ggcccccgtg acgccaacgg cattccggtc
    16621 ccgatgacgg tggatgaatc catcgccagc atgaaggcgt cgttactgaa aaaaatcaaa
    16681 cgttctgcct acgtttaccg cgtggactgc ggtggctgca acggctgcga aatcgaaatt
    16741 ttcgccacgc tttcgccgct gtttgatgca gaacgcttcg gcattaaagt cgttccttca
    16801 ccgcgtcatg cggatatttt actgtttacc ggcgcggtca cccgtgcaat gcgatcccct
    16861 gcgctgcgtg cgtggcagtc cgcgccggac ccgaaaatct gtatctccta cggtgcctgc
    16921 ggtaacagtg gcgggatctt ccacgatctc tactgcgtgt ggggcggtac ggataaaatc
    16981 gtcccggtag atgtctacat ccctggctgc ccgccaacgc ctgccgctac gctatacggc
    17041 tttgcaatgg cgctcggcct gctggagcag aaaattcacg cccgtgggcc gggtgagcag
    17101 gatgaacaac cggcggagat cctgcatggc gatatggtgc agccgctgcg tgtgaaagtg
    17161 gatcgcgaag cacgccgcct ggcgggttat cgttacggtc gtcagattgc cgatgattac
    17221 cttacacagt tagggcaggg cgaagaacag gttgcacgct ggctggaagc ggaaaacgat
    17281 ccgcgtctga acgagattgt cagccatctg aatcatgttg ttgaagaggc gcgtatccga
    17341 tgagtgaaaa ggtggtgttc agtcaactga gccgtaaatt tattgatgag aacgatgcta
    17401 cgcccgccga ggcgcagcag gtggtctatt acagcctggc gattggtcac caccttgggg
    17461 ttatcgattg cctggaagcg gcgcttacct gcccgtggga tgactatctg gcatggattg
    17521 ccactctgga ggcgggcagc gaagcccgcc gcaaaatgga aggcgtgccg aaatatggtg
    17581 agatcgtcat cgacattaac catgtgccga tgctggctaa cgcattcgat aaagcccggg
    17641 cagcgcaaac ttcgcagcag aaagaatgga gtacaatgct gttaagtatg ctgcatgata
    17701 ttcatcagga aaacgccatc tatttgatgg tgaggagact gcgtgactga cgttttactt
    17761 tgtgttggca atagcatgat gggcgatgat ggcgcaggcc cgctgctggc ggaaaagtgc
    17821 gccgccgcgc cgaaaggtaa ctgggtggtg attgacggcg gtagcgctcc ggaaaacgac
    17881 atcgtcgcca tccgcgaact gcgcccgaca cggctgctga ttgtcgacgc cacggatatg
    17941 gggttaaacc ccggcgagat ccgcatcatc gatccggatg atatcgccga gatgtttatg
    18001 atgactaccc ataacatgcc gttgaactac cttatcgacc agttaaaaga agacattggc
    18061 gaagtgattt tcctcggcat ccagccggat atcgtcggct tttactaccc gatgacccag
    18121 ccgattaaag atgcggtaga aaccgtttat caacgactgg aaggctggga agggaatggc
    18181 ggattcgcgc agttagcggt ggaagaagag tagtttttca gtaaggaatc aggacaggga
    18241 tgttcttgat gagggaatca gaactgatag caaacgccag attgcccgat gcgctgcgct
    18301 tatcgggcct acctggttcg tgcgacatgt aggccggata aggcgttcac gccgcatccg
    18361 gcactgttac ttactctaaa tcctcaccat tactggcaat cactttttta taccaccaga
    18421 acgatttctt acgcgtgcgc gccagcgtac cgttgcctgc gtcgtcacgg tctacgtaga
    18481 caaaaccgta gcgtttgctc atttcacccg ttgaggcgga aaccaaatca atacagcccc
    18541 aagtggtgta gcccatcagc ggaatgccat ctgcaatcgc ttcgcccatt gcgcggatat
    18601 gttcgcgtaa atagctgatg cgatagtcat cgttaatctc gccattggca gcaagttcat
    18661 cttttgcgcc gaggccgttt tccaccagaa acagcggctt ctgatagcgg tcatacatca
    18721 tgttcatggt gatacgcaga ccgagtggat caatccccca gccccagtcg cttacctgta
    18781 gatatgggtt acgcagcgat ttcaccacgt tcgccgcact gctgttgttg gcgttcatct
    18841 ccgccgaggc gcagcgtgag gcgtaatagc tgaaagagac aaaatcgacg gtgtttttca
    18901 ggatctcatc atcgcccggt gctttgtcga tggttacccc tttttcgcgg aatacgcggg
    18961 cagagtaagc cggatacgcg ccccgcgcct gcacatcgat aaaaaacagg ttttcccgat
    19021 ctttctccag cgccgcccag acatcttccg gcttgcaact gtaagggtag aagttaccgc
    19081 ccgccagcat acagcccacc tgattttgcg ggttaacctc atgggcgatt ttggtggcta
    19141 atgcactggc aatcagttgg tgatgcgcag cctgatattt cacctgatcc tgattttcgc
    19201 cttcttcaaa caccagaccc gcgccggaga acgggctatg caacataatg ttgatttcat
    19261 tgaaggtgag ccagtatttc accagaccat caaatgcttc aaagcaggtc cgggcgtagc
    19321 ggctgaaaaa ctccaccagc ttgcggttac gccaggagcc atattcggtg accagatgca
    19381 tcggcacatc gaagtggcac aacgtgacca gcggttcgat accgtacttt ttacactctt
    19441 caaagacaga acggtaaaaa gcaatgccct gctggttggg cgtcagttca tcgccctgcg
    19501 ggaagaggcg gctccaggca attgaggtac ggaacacttt gaatcccatc tctgccatca
    19561 gggcgatatc ttctttataa cgatgataaa aatccgtcgc ctcatggctg ggataaaact
    19621 cgtcatcgcg caactgaaaa cgtttttcca gccccagttt caccgccatt cgatgctcgc
    19681 cgtgtgggat catatcgacg gtggtcagcc ctttgccacc ttcccggtac gcaccttcag
    19741 actggttggc ggcaagcgcg ccgccccata aaaaaccttc tggaaatact gacattttca
    19801 tcctcaatta agacttactt ctttggcgac ggtcggttgt gcgctctgat gctttcgcgc
    19861 ctcggcagtc gcttcctcaa caggaatatc ctcaaagccg agcaacagtg tgaggatgaa
    19921 cgacagcacg accgccagcg ccatgacggc gaacacccag acgatgctca ttggattcgc
    19981 cggatcgaag aactgcacgc tggtaaatag ccccggcgct gccattgagt ggctggcaag
    20041 ccccgccata ccggcaaccg cgccgcaaat aaaaccgctg ataagactgg cgataagcgg
    20101 acgtttcagg cggatcgcca cgccatataa cgccggttcg gaaatccccg ccataatggc
    20161 tgatgccgcc gcagccagcg ccgtctgacg cagttccggg tttttcgttt tccacgccac
    20221 tgccagtgat gaaccgccca gcgacaggtt agcgccgatc tctgacggca tgaccatccc
    20281 ttctttgccg gtttcggcaa tggtctgaat gatggttggc gtaaagacgc ggtgcatccc
    20341 ggtcattacc agcagaggcc acagcgcgcc cataatggcg actgaaagcc agcccagata
    20401 accatgaatg gtgtaaacca gcgccgaaat ggcgctaccg atccagatac caatcgggcc
    20461 aatcagcagg attgccagcg gtgcggcaat caacacaatc agcattggct tgaggaagtt
    20521 tttagtcacc gccggtgtaa tgcgatccac ccagcgttcg atatatgaca ggcaccaggt
    20581 catcaccagc gccgggatca ccgtgtaggt gtatttcacc gcggtgaccg ggatcagggc
    20641 aaattcgaca tgttcaccct gggccgcttt cgccatcagt tcaataaaac tcggatgtac
    20701 cagaacaccc gcgatggcaa tcgccagcga catattggtt ttaaatttga tggcggccga
    20761 agccgcgacc atcagcggca ggaagaagaa agcaccgtca ccaatcacat tcagaatggt
    20821 taaggtcggc gatccttttg tcagcacgcc gctcatctcg aggatcatcg ccagcagttt
    20881 gaccatcgat ccgccgataa tcgccgggat cagcggtgac atggtgccga tcagcgcatc
    20941 gaggatcccc gcacctatgc gacgtagcgt aagtttgggt ttacccacgg gctgtgcggg
    21001 ctgcatatct cccggcagca ggctgacgac ttcctgaaag gcttgtgaaa cggtattgcc
    21061 gataatcacc tgacactggt tgtcactacg taccacgccg agcacgccgg agatggtttt
    21121 caacgtcgga ctgtcgataa gctggtcatc tttgataaca aagcgcaaac gcgtcataca
    21181 gtgcgtgact gccgagatgt tatcaacgcc gcccagtgcc gctatcaccg agcgtgccag
    21241 cgccgcataa tttttggcca tcggatttta tcctgttatc agtagggtag atgtagttgt
    21301 tatcagtcga cgtgacggaa aaatgccgat gctgaaacgg aattaggaaa ccggtttcac
    21361 aaaatcatga atagttgtca ctgaataagc aagagagtta attgttcatt ttttatattt
    21421 gtgaaaccgg tcacctgaat atttttcccg ccgattgcgc aaggacaatc ggcaaacggt
    21481 gcagtacact gcgttgattg cgtgtaaagg gatgaaaatg atgacgacga tgctggaagt
    21541 ggcgaagcgc gccggggttt caaaagcgac cgtttcccgc gtgctttcag gtaatggcta
    21601 cgtcagccag gagactaaag atcgcgtgtt tcaggcggta gaagagagcg gttaccgtcc
    21661 aaacttgctg gcgcgcaatc tgtcggcgaa gagtactcag acgctggggc tggtcgtgac
    21721 caacacgctt taccacggca tttattttag tgaattactc tttcatgccg cgcgaatggc
    21781 ggaagagaaa gggcgacagt tgctattggc agatggtaaa cacagcgccg aagaagagcg
    21841 ccaggcgatt cagtatctgc tggatttgcg ctgcgacgcc attatgattt acccgcgttt
    21901 tttaagcgtg gatgagattg atgacatcat tgacgcacac agtcagccga taatggtgct
    21961 taatcgccgc ctgcgcaaaa acagcagcca tagcgtctgg tgcgatcata aacagaccag
    22021 ctttaacgcc gtggcagagt tgataaacgc cggacatcag gagattgctt tccttaccgg
    22081 ttcgatggat tcccccacca gcgttgaacg tcttgccggg tataaagacg cgctggcgca
    22141 gcatggtatt gcgctcaatg aaaaacttat cgctaacggt aaatggacgc ctgccagcgg
    22201 ggccgaaggg gtagaaatgt tgctcgaacg tggggctaaa tttagcgcgt tagttgccag
    22261 taacgacgat atggcgatag gtgcgatgaa agcgttacac gagcgcggcg tagcggtgcc
    22321 agagcaggtg tcagttatcg gattcgatga tatcgctatt gccccctaca ccgttccggc
    22381 gctctccagc gtaaaaattc cggtgactga gatgattcag gaaataattg ggcggctgat
    22441 atttatgctc gatggtggtg cttacacacc gccgaaaacc ttcagcggaa aactgatccg
    22501 ccgcgactcc ctcattgctc tttcgcgata aacattgtca tcacatgtca tcgtcactag
    22561 tgtcgatgac atcgtttttc ctgcattaca gaatttaatt tctttaaata acagtaaatt
    22621 aaaaactggc atgatttgtg aatgtatcgg cgcattaact gtcattgctg gagaatttga
    22681 tgaaccgttt catcattgct gacgcgagta aatgtattgg ttgccgtacc tgtgaagtgg
    22741 cgtgcgtggt ttctcatcag gaaaatcagg actgtgcatc gctgaccccg gaaacttttt
    22801 taccgcgaat ccatgtcatt aaaggtgtga acatttccac ggcgacagtt tgccgtcagt
    22861 gtgaagatgc accgtgcgct aacgtctgtc cgaatggtgc tatcagtcgt gataaagggt
    22921 ttgttcatgt gatgcaggaa cgttgtattg gttgcaaaac ctgcgttgtg gcttgcccgt
    22981 atggcgcgat ggaagtggtg gtacgtccgg tgattcgcaa cagcggcgcg gggctgaatg
    23041 tacgggctga caaagccgaa gccaataaat gcgacctgtg caaccatcgt gaagacggcc
    23101 cggcgtgtat ggcggcttgc ccgacccatg cgctgatttg tgtcgatcgt aataaacttg
    23161 aacaactgag cgcagaaaaa cgtcgccgta cggcgctgat gttctaaaaa tcttactgaa
    23221 ctgttctggc atcaaaggaa ttcccggaaa tattcgtaga atacgaagcg ggcagtatcg
    23281 ccgacctgtg actgccggat gcgacgctgt cgtgtccggc tgggcgggtt acgcaattca
    23341 tgatggcggg aataactcaa tggcaaaaaa cacatcttgc ggtgtccaac tgcgtattcg
    23401 tggcaaagtg cagggcgtcg gttttcgtcc gtttgtctgg caactggcac agcaattaaa
    23461 tcttcacggc gatgtctgta atgacggcga tggcgtagaa gtccggctgc tggaagatcc
    23521 ggaaacgttt cttgtgcagt tgcatcagca ctgcccgcca ctggcgcgta ttgatagcgt
    23581 cgagcgtgag ccgtttatct ggtcacaact gcccactgag ttcaccatcc gccagagcgc
    23641 gggtggcgtc atgaatacgc aaattgtccc ggatgccgcg acttgccctg cttgtcttgc
    23701 cgaaatgaat accccaggcg aacgacgctg gcgttatccg tttatcaact gtactcactg
    23761 tggtccgcgt ttcaccatta ttcgcgccat gccttacgac cgcccgttta ccgtgatggc
    23821 ggcgtttccg ctgtgtccgg cctgtgacaa agagtaccgt gacccgctcg atcgtcgctt
    23881 ccacgcccag ccggtggcct gcccggagtg tggcccccat cttgaatggg taagtcatgg
    23941 tgaacatgca gaacaagagg cggcattaca ggcagctatc gcacagttaa aaatgggcaa
    24001 cattgtcgcc atcaaaggga ttggcggatt tcatctcgcc tgcgatgcgc gtaacagtaa
    24061 cgcggtggcg acactgcggg cgcgcaaaca tcgcccggcg aaaccgctgg cggttatgtt
    24121 gccagtggca gaaggtttac cagacgctgc ccgtcagttg cttaccacac ccgccgcgcc
    24181 gattgtgctg gtggataaaa aatacgttcc tgagctttgt gatgatatcg cccctggcct
    24241 taacgaagtt ggggtgatgt tgcctgcgaa cccgctccag catttgctgt tacaggaact
    24301 gcaatgcccg ctggtgatga cctccggcaa cctgagcggt aaaccaccgg ctatcagcaa
    24361 cgaacaggcg ctggcggatt tgcagggcat tgccgacgga ttcttgatac ataaccgcga
    24421 catcgtgcag cggatggatg attcggtggt gcgcgaaagc ggcgaaatgc tgcgccgttc
    24481 gcgggggtat gtgccggatg cgctggcttt gcctccgggc tttaaaaatg ttccgcctgt
    24541 gctgtgtctc ggcgcggatc tgaaaaatac cttctgcctg gtgcgcggtg aacaagcggt
    24601 gttgagccag catctgggcg atttaagtga cgatggcatc cagatgcagt ggcgcgaagc
    24661 gttacgcctg atgcaaaaca tctacgattt caccccgcaa tacgttgtgc atgacgcgca
    24721 tccgggctat gtctccagcc agtgggcgcg tgagatgaat ctgccgacgc aaacggtgct
    24781 gcatcatcat gctcatgcgg cggcgtgtct ggcagaacat cactggccac tggatggcgg
    24841 tgatgtcatt gcgttgacgc tcgacggtat cggtatgggg gagaacggcg ctttgtgggg
    24901 cggcgagtgc ctgcgggtga actatcgcga atgccagcac ctgggcggct tgcctgcagt
    24961 ggcgcttgct gggggcgatt tggcggcgaa gcagccgtgg cgtaacctgc tggcgcagtg
    25021 cctgcgcttt gtgccggagt ggcagaatta ccctgaaacg gcaagtgtgc aacagcaaaa
    25081 ctggagcgtg ctggcgcggg ccattgagcg tggaattaac gcgccgctgg cgtcatcgtg
    25141 tgggcgtttg ttcgatgctg tggcggcggg actgggctgt gcgccagcca cgttaagtta
    25201 tgaaggtgaa gcggcttgtg ctctggaggc gctcgcagct tcgtgcgacg gagtgacgca
    25261 tccggtgacg atgccgctgg tggacaatca actggatctc gccactttct ggcagcagtg
    25321 gctgagctgg caggctccgg ttaatcaacg cgcgtgggcg tttcatgatg cgctggcgca
    25381 gggttttgcc gcgttgatgc gtgagcaggc cacgatgcgg ggtatcacta cgctggtatt
    25441 tagcggcgga gttattcata accgtttgct gcgtgcacgt ctggcgcatt atcttgctga
    25501 ttttacgttg ctcttcccgc agagtttacc ggcgggcgat ggtggattgt ctctggggca
    25561 gggggttatt gctgcggcgc gctggttagc gggtgaagtc cagaacggat aaagcgtgtt
    25621 gcatttggac accattgccg gatgcggcgt aaacgcctta tccggcctac acgtgcgtcc
    25681 ccgtaggtcg gataagacgc gccagcgtcg catccgacat tttgggacag tgcaggatgc
    25741 gacataaacg tattatgtga tcaacacatg cgttccctgt aggtcggata agacgcgtca
    25801 gcgccgcatc cgacatttgg ggcactgtag ctcgcctaca ccggcaacgt tttcaacaat
    25861 ccaaacgcct ctttcatccg atcctcactg accacaaagg cgcgaagctg gtcggcatca
    25921 tcaacaccgc gcgccaccat tccctggcgt tcggtattaa tttgccagcg taaatcctga
    25981 cgctgggttt cgcctgccag atgcagcggt aattccggcg ttttgatttt caccagcatc
    26041 gccgggagtt tcagcggcgt gttattgccg agaagatttt ttgccagcac catcgcgcta
    26101 agttgaatcg gctggaggaa cggcaatacc tgaccgttaa tttccgcgca atcgcccagc
    26161 gcataaatat cggcattact ggtttgcaga taactatcga cgcaaacgcc gcgattaatc
    26221 gtcagcccgg cgcgtcgtgc cagggcggtt tccgggcgca gtccggtggc ggcaattacc
    26281 gcatccactt cgatgctgcg ctggcggtcc agcgttgcca gaatgccaga atccgttttt
    26341 tccagcccct gcaactgaga ttttaacagc agatgaacgc ccatctccgt caaccgatgc
    26401 tgcaagcggc tgcttacttc cggtggcatt aacgctgcca gaatactggc agcgttgtcg
    26461 attagcgtga ccgctttgcc tgcacgacaa aaatccatcg ccagttcgct accaatcaag
    26521 ccaccgccaa caatcaacac gcgtcgggca ttccgcagtt gcgtttcaca ggcgcgatac
    26581 tcttgctgac tatttaacgt cagcattaac tcacgcccgg gcactggcgg gacaaaggcg
    26641 ctggcaccgg ttgccagcac taacttgtcg tattgccact gattattctg acttttcacc
    26701 acatgggctt cggcatcgat atccgttacc caggtgtgcg gaaacaggtg cagattaaac
    26761 tgctcggcaa attcacccgc cgtctggcgg gtaaggtcat cggcacgttg cctctggctg
    26821 ataacatggc tgaggtccgg tttgttgtac tcatccatgc tgtcggcggc aatcagggtt
    26881 aatggaatac aggcgtcctg tttgcgaata tttttcacca gttggcgggc ggcgaagccc
    26941 gaaccaatga tcacaatgcc gttgctcatt ttgcctccga tgccagttcg tcaaagacgt
    27001 ctttgccaag ggagcattcc gggcacagga agttatccgg aacttcactc cacggcgttc
    27061 ctggcgcaac gtcctgcatt ggctcgcctt ttgccggatc gtaaatccac tggcagacgc
    27121 tgcactgcat ccgtgggccg aggtcagccg tcgtggtggc agaggtttct tctttaacca
    27181 ccgtattcac cgtgctctgc ggcagcggcg cgagcgccca ctgacgggcg atttcgcgac
    27241 cgtgttcacg acatagctcc agagcgtcct ggtccggtcg ccatttggct tttaggctaa
    27301 gcgacatttc gaaacccgca tcctgcaggc gtgtggaaag acgatccacc gcaccgccgc
    27361 tccagccgtg agagccgaaa gcactggcgc gtttgttacg aaagcgtaaa ccagtcatct
    27421 cctccaccag cccggcgatt tttggcatca tcacgttatt catcgtcgaa gtgccgacca
    27481 gcacgccttt tgagcggaag acattggtca ggatttcgtt tttatcgctt cgggcgacgt
    27541 tgaaaatttt caccgctacg cgtgggtcgg tttccgcaat accctgggct atagcatcgg
    27601 ccatcatgcg ggtattattc gacatggtgt cgtagaaaat ggtgattctg tcttcctgat
    27661 aatccgccgc ccatttcagg tacagctcga caatttgcgt cgggttatcg cgccatacca
    27721 cgccgtgaga ggtcgctatc atatcgactg gtaagttaaa gcccaggatc tcggtaattt
    27781 tcggcgttac caggcggctg aacggcgtca ggatattggc gtaataacgc tggcactgct
    27841 cgaaaagctc cgtctgatcc acttcatcgt tgaacagatg ctcgtcgcag tagtgctgac
    27901 cgaaagcatc gttactgaac agtaccgcgt cgcctgtcag gtaagtcatc atgctgtccg
    27961 gccagtgcaa cattggcgtt tcgacaaaaa tgagctgttt gccgttgccg atatccagcg
    28021 tgtcgcctgt tttcaccaca ttaaaattcc actccggatg atggtgatga ccatttatcg
    28081 agtcgatagc gttggcagta cagtagatcg gcgtatcggg aatttgtgcc atcagttcgg
    28141 tcagcgcccc ggcgtggtcc tcttcagcat ggttaattac gatgtaatcg atatccgtca
    28201 gatcgatttc attacgcagg ttctgcacaa attcgcggct gaatttatgg tcgacggtgt
    28261 cgatcagcac gtttttttct tcgcggatga ggtagctgtt gtagctgctg ccgcgcagcg
    28321 ttttatattc tgtaccgtga aaatcacgca cttcccagtc acgttgacca acccaatgaa
    28381 tgttattttt caccacaata gacatagcaa cctcaattta ttcagcgtgt tctaaaaaga
    28441 tgtcttgctg attgcagatt gcgtgccagt tttttatcta attgttttta ctctataaat
    28501 aaaattgtgc tcattaatga gtagtcaaaa tgactatcaa taaaatggtc gatatgacaa
    28561 tatctatagt caaattgaca gtgaggcaaa gatgagtttt tccgttgatg tgctggcgaa
    28621 tatcgccatc gaattgcagc gtgggattgg tcatcaggat cgttttcagc gcctgatcac
    28681 cacgctgcgt caggtgctgg agtgcgatgc gtctgcgttg ctacgttacg actcgcggca
    28741 gtttattccg cttgccatcg acgggctggc gaaggatgta ctcggtagac gctttgcgct
    28801 ggaaggtcat ccacggctgg aagcgattgc ccgcgcgggt gatgtggtgc gctttcccgc
    28861 agacagcgaa ttgcccgatc cttatgacgg tttgattcca gggcaggaga gtctgaaggt
    28921 tcacgcctgc gttggtttgc cgttgtttgc cgggcaaaac ctgatcggtg ccttgacgct
    28981 cgacgggatg cagcccgatc agttcgatgt tttcagcgac gaagagctac ggctgattgc
    29041 cgcactggcg gcgggagcgt taagtaatgc gttactgatt gaacaactgg aaagccagaa
    29101 tatgctgcca ggcgatgccg cgccgtttga agcggtgaaa cagacgcaga tgattggttt
    29161 gtccccaggc atgacgcaat tgaaaaaaga gattgagatt gtggcggcat ccgatctcaa
    29221 cgttttaatt agcggtgaga cgggaaccgg taaggagctg gtggcgaaag cgattcatga
    29281 agcctcgcca cgggcggtga atccgctggt ctatctcaac tgtgccgcac tgccggaaag
    29341 tgtggcggaa agtgagttgt tcgggcatgt gaaaggggcg tttactggtg ctatcagtaa
    29401 ccgcagcggg aagtttgaaa tggcggataa cggcacgctg tttctcgatg agatcggcga
    29461 gttgtcgttg gcattgcagg ccaagctgct gagggtgttg cagtatggcg atattcagcg
    29521 cgttggcgat gaccgcagtt tgcgggtcga tgtgcgcgtg ctggcggcga ctaaccgcga
    29581 tttacgcgaa gaggtgctgg cagggcgatt ccgcgccgat ttgtttcatc gcctgagcgt
    29641 gtttccactt tcggtgccgc cgctgcgtga gcggggcgat gatgtcattc tgctggcggg
    29701 gtatttctgc gagcagtgtc gtttgcggct ggggctctcc cgcgtggtat taagtgccgg
    29761 ggcgcgaaat ttactgcaac actacagttt tccgggaaac gtgcgcgaac tggaacatgc
    29821 tattcatcgg gcggtagtac tgtcgagagc cactcgcagc ggtgatgaag tgattcttga
    29881 ggcgcaacat tttgcttttc ctgaggtgac gttgccgccg ccagaagcgg cggcggtgcc
    29941 tatcgttaag caaaacctgc gtgaagcgac agaagcgttc cagcgtgaaa ccattcgtca
    30001 ggcactggcg caaaatcatc acaactgggc cgcctgtgcg cggatgctgg aaaccgacgt
    30061 cgccaacctg catcggctgg cgaaacgtct ggggctgaag gattaaataa tcccggcctg
    30121 atagaaatcc tgcaggttaa ttgcgccggt gagtttgccg ttttcatcca ctaccggtgc
    30181 ggcggtgatt ttgcgtttca tcaggatttc tttggcgtcg atggcacggc tttgcgattg
    30241 caacgtggtg ccgccgaccg tcatcgcttc attgactggc gtggtgagtg cgccgccgcc
    30301 aaccagccag cgacgtaaat caccgtcggt aaagacgcct ttcaccagtc gttgatcatc
    30361 gcacactgcc accagaccta aaccggtgcg gctaagttcc agcatcgcgt ccatcacgct
    30421 ggcggttaac gccacctgcg ggatggcatc gtcacggcgc atcagatgat gcactttatt
    30481 cagcaagcga gcgcccagtg ctccagcagg gtgggagcgg gcaaaatctt cttcattaaa
    30541 tccgcgcgcc tgcatgaccg ccatcgccag cgcgtcaccc atcatcaggg tattgacggt
    30601 gctggaggtc ggcgcaaggt gcatcgggca ggcttcgcgt tctacggaga tatccagcac
    30661 cgctttcgcc gccaggccca gcggtgacgt cggtttgccg gtcatcgcca gcagcgcgat
    30721 agatttatct tccagacgcg gaataatcag atccagttcc ttcgcgccac cggagtaaga
    30781 gataaacagc atcacatcgc ggctttcgat catccccagg tcgccgtgca atgcctctgc
    30841 cggatggaca aaaaaggcag gagtgccggt gctggcaagc gttgcggcga ttttcttacc
    30901 aatgtggccc gatttgccaa ttcccgaaac caccactttg ccttcgcagt gcaggatgat
    30961 attggcggcg cgaacaaaat catcgcccag acgttccggt aaacggcttg cttcctgcaa
    31021 ctccagcatt aacgtctgac gtcccgcgtt cagtagtgct tcactcattg ctctctccgg
    31081 ttatgatcac gtcgatccct ttcttttcca gcgcctgacg aaacgctgga tcgatacctg
    31141 cgtcggtaat tagcttatcg acgctttcaa gactgcaaac tacgttgggg cttttacggc
    31201 caaactttga tgagtccgcc atcaaaatca cttcgcgcgc ggcattgcac attgccttac
    31261 tgacggtata aacctcgttg aacgtggtta cgcccgcatt gagatcgatg ccgtcggtgc
    31321 ccataaacaa tttatcgaag gtgaaatgct cgaaggcatt ctctgccagt tgcccgtgaa
    31381 atgaggccga ttttttacga aatgttccgc ctggcatcag gatagtttgt tcgttatcca
    31441 gttcggatag cgcattgacg atatgcaggc tgttggtcat cactgtgatg ttattaaagc
    31501 gcgagagcag ggggaccatc tgcaaaacgg tactgccagc atcaagaatg atcgaatcgc
    31561 catcatggat aaaactaacg gcagcttctg caatcagctc tttcttgtgg gtgttgatga
    31621 gtgttttatg atcgattggc ggatcggatt cctctttatt caacaccact ccgccataag
    31681 tacgaatgac ggttccggca tgttccagaa tgaccagatc tttgcgaatg gttgtgcctg
    31741 tggtgtcaaa gtattgcgcc aattcttcaa ccgagcattt accctgcttt tgcagatact
    31801 ccagaatggc ggcctgacgc tgacgaggtt tcatgggcgt gattacctga tttaggtttc
    31861 aaaatgataa cttcgcaagc aagtaacatt cacaacgaaa ttatccacgt ttcagtttaa
    31921 gcgccaatga tagagcattc tgtgatagtg gatcatgggg aaagatcaca tcgggctgta
    31981 aatcacccgc atacataccg gtaattgcgg gaattttttg tggtcgggcg aagacggtca
    32041 gtcctttcat aaacaaggtg tcgacgatgc gttgctgatc gtccagggcg atggcgacca
    32101 cgacccgcgg tttaaagcgc ccgctggaac ggcccacgcc aacccgccct tgctggcata
    32161 gtgtgtcgaa ggcacggtta aaacgagaaa tttgccaacc gcctaaggcc agttgcgcac
    32221 accaggcgat aacggcgacg gtgatgagtg cggataccat aacttttcct taatgtagtg
    32281 gcggaagtcc tgtatgcctg atgagcgtag cgcatcaggc attttattag tctcgcggag
    32341 atccttaacg gatctccgct gttaatcaga acatcacctg accgccggtg acattgatcg
    32401 actgaccagt gcagtacgac gccttaggac tggcgtagaa cagcagcata ttcagcacat
    32461 cctgataatc gcagccgcgt ttgagcggta ctttgtcgat gtaatactgc tcgacttgat
    32521 ccggtttgat acccagcttg gtcgcatatt gtggcaatag cgactgaaac atcggcgatt
    32581 tcagcagatt accgagcatc agtgaatgca ccgtaatgcc gtactccgcc agatccagcg
    32641 ccagtgattg agtcagcccg acgccaccaa atttcgctgc gctgtagcca gagttgtgtt
    32701 tgctgcccac tttgccggat ttcgagttga tctgaataat gcgcccctga atcccgtcgc
    32761 ggatcatcaa acgcgaaaat tcacgggcac acaggaaata acccaccaga ttcacctgta
    32821 gcgaacggtc aaaatcgccg agctggaagt cgctgataaa ggctgctttt gctattccgg
    32881 cgctgtagac cagcaaatcc acgcgaccaa agatttcatc taccccacga gagagcgcca
    32941 gaacgctttg ctcgctagtg gcgtcagcac caaaaccgta cgccatacct tcaccatatt
    33001 cggcgttaat ttcttgtgcc acatttgcgg ctttgtcgct ctgaatatcg acaaccgcga
    33061 cgcgataccc ctcggcagcc agaccgtggc acaggaacgc gcctaaggtt tgcccaccac
    33121 cgatgacaac ggcaacctga ttcatttttt actccttaac agattcaaac ttcaaaaccg
    33181 atcccggcgc gatatcgtcg ggtacagggc ctgccacatg gacagtgccc ggaaattccg
    33241 cttcgtttaa accatcgaag cgcagggtga catgacccag ttcgcgaagg ttgtcttccg
    33301 ccacgctgcc aacggcggtc accggatagc gatgctgccc gagtgaaaat tgcaaaccgg
    33361 gatggagtgc acctttcaac tcgccgtggc aatgaatgaa gcaatactct tcgagatccg
    33421 ctggcgcgcc ttcacgaaag gtgatgagca tctggtcgct gagggcgtca gtggcactcg
    33481 cgccaatacg ggtgatggtg gtctgataaa taacggtcat gtttcagcct ctattgatag
    33541 ataaaaccgg agacaaacca ggcgatcagt acagttggcg caccggttaa aaagcggctc
    33601 accagaacag aagggacacc gacgcgaacc gtgtcctgac gggcttccgc cagcgacaaa
    33661 ccgaccggga tgaagtcgca ggccgcctgc gcgttgatgg caaacagtgc aggtaaagcc
    33721 agatgcggcg gaatattgcc gagaccaatc tgcacgccaa tcaatacgcc gataacctgt
    33781 gcgataactg cgcctgggcc gaggaaaggt gaaagcagcg ggaaggagca gatgagcgcc
    33841 agcatgacca gacccagtgg atggctcgcc agcggagcaa gaccgtgggc aatccagtcg
    33901 ccaagaccgg aagccataat gatgccgata agcgccgaaa cgaatgccat aaacggcaga
    33961 atggttttta atacagtgtc gatggtgtca cgaccagatt gaaacaacac ggcaacggca
    34021 gaacccatac ccattcccac cttcgccagt aaaccatcgc tttgttcggt gattttcttg
    34081 ctggtgtcat agtcacggcc cacggaagag ggttgtggtg tcgcatcacc cactacagtg
    34141 atgttttctt cttttacgcc agagacataa atatcttcta caatgtattg cgctagtgga
    34201 ccggacttgc ccgtcgagtg gatattaatg gtgggaatac gccgtttcgg ataaatgccg
    34261 cagcgtaatg tgccgccaca gtcgattacc gcgacaccaa tttccgcctc cgcgggttca
    34321 ccttctttaa atccgtcaat agcctgccag ccagtaagct gtgccagttt gtcaacaatc
    34381 gcaggccggg taccggcggt gatatagacg atttttttgc ccggcgtggc ttccagctca
    34441 agcgggccgc cccagccacc cgttcctttt tcgatccgaa tacgcgtcat gatgttgctc
    34501 ctgccagatg aactttttgt tcaagttgaa tgcccatctt tttctcaaaa atggcggtgg
    34561 tcagatcggt cacccagccg cggaagaagt tggtgaccag accaaccagc agataactca
    34621 ccgccagtgg gccaagtggc aggttcagtg ttgtcagacc gctggcaatg ccaagataaa
    34681 caaacagttc gcctgggttg atatggggga agaggccatt cattgagtgg cagctataag
    34741 aggcggccgc gtagtagctg ggtttgtact tttccggcat aaagcgaccc aggcttaagg
    34801 tcatcggatt gcaaaagaca aacgtgccaa tgcacggtaa cagcaggtaa cgggaaacag
    34861 ggttaccggc gcaacgttga gcaaaacgtt caatacgatg ctgaccgata aaattaatca
    34921 gtgcgttcat gataaccagc agactaatca acagcggaag aatgccggtc accatcccgg
    34981 taaacacctc tccgcccttt tggaacagcc cgataaacca ctctgcacca tgagtaatgg
    35041 tttctatcat tgttctctcc ttcaggattt attgttttat taccaaacgg caacctaatc
    35101 taatcagatt gaaagattta aaagtgttat tttgatcgca aaatgaaaga taaatatttt
    35161 aatttgaaag tttgaataaa aggatagcga ggagaatgag ttgagttatg taaagtccgt
    35221 atcgggcagt gactaccgct tccttgtgcg gggcgtgatg ctttaccata cttgcccctg
    35281 gttgaatctg ttaaatggac ccctcatgtt caagcgtcgt tatgtaacat tgcttcccct
    35341 ttttgtgttg cttgccgcct gtagcagcaa gccaaaacct actgagactg aaacgaccac
    35401 cggaacgccg tctggcggct tcctgcttga gccgcagcac aatgtgatgc agatgggcgg
    35461 tgacttcgcc aataatccga atgcccagca gttcatcgac aaaatggtga acaaacacgg
    35521 tttcgatcgt cagcagttgc aggaaattct ctcccaggcg aaacgtctgg attcggtact
    35581 gcggctgatg gataaccagg caccaaccac atcggtgaaa ccgccatcag gtccgaacgg
    35641 cgcatggctg cgttatcgca aaaaatttat tacgccggac aacgtgcaga acggtgtggt
    35701 tttctggaat cagtatgaag atgcgttgaa tcgcgcgtgg caggtgtatg gtgtaccgcc
    35761 ggaaattatc gtcgggatta tcggcgttga aacccgctgg gggcgcgtga tggggaaaac
    35821 tcgcatcctc gatgcgctgg caacgctgtc atttaactac ccacgccgcg cggagtattt
    35881 ttctggcgag ctggaaacct tcctgctgat ggcgcgcgac gagcaggacg atccgctcaa
    35941 tctgaaaggt tcctttgccg gggcgatggg ctacggacag tttatgccgt cgtcttacaa
    36001 acaatatgcg gtagatttca gcggcgacgg gcatatcaac ctgtgggatc cggttgatgc
    36061 cattggtagt gtggcgaact atttcaaagc gcacggctgg gtgaaaggtg atcaggttgc
    36121 ggtgatggca aacggtcagg ctccaggctt gccaaatggc ttcaaaacca agtacagcat
    36181 ttcgcagctt gccgcggcag gtttaacgcc acagcagccg ctgggcaacc atcaacaagc
    36241 cagcctgctg cgtctggatg ttggcaccgg ctaccagtac tggtacggtc tgccgaactt
    36301 ctacaccatt acccgttata accacagcac ccattacgca atggcggtct ggcagttagg
    36361 acaagccgtg gcgctggcgc gcgtacagta actatttgta agaggggggc gtgagcttcc
    36421 ctctggctaa tcagcttttt tctgaatcct cctcgtaaaa ttgcaacgcc aacaccatct
    36481 tcctgacgaa agtgttatct tgtccggcat aaatttttga ctgacagagg ttgtgatgac
    36541 tgacagtgaa ctgatgcagt taagcgaaca ggttgggcag tcgctgaaag cccgtggcgc
    36601 aaccgtaaca actgctgagt cttgtaccgg cggttgggta gcgaaagtga ttaccgatat
    36661 tgccggcagt tccgcctggt ttgaacgcgg atttgtcacc tacagtaacg aagcaaaagc
    36721 gcaaatgatc ggtgtacgcg aagagacgct ggcacagcat ggcgcggtga gcgaacctgt
    36781 cgtggtggaa atggtgatag gcgcactgaa agcggctcgt gctgattacg ccgtgtctat
    36841 tagtggtatc gccgggccgg atggcggcag tgaagagaaa cctgtcggca ccgtctggtt
    36901 tgcttttgcc actgcccgtg gtgaaggcat tacccggcgg gaatgtttca gcggtgaccg
    36961 tgatgcggtg cgtcgtcagg ctactgcgta tgcattgcag accttgtggc aacaatttct
    37021 acaaaacact tgatactgta tgagcataca gtataattgc ttcaacagaa catattgact
    37081 atccggtatt acccggcatg acaggagtaa aaatggctat cgacgaaaac aaacagaaag
    37141 cgttggcggc agcactgggc cagattgaga aacaatttgg taaaggctcc atcatgcgcc
    37201 tgggtgaaga ccgttccatg gatgtggaaa ccatctctac cggttcgctt tcactggata
    37261 tcgcgcttgg ggcaggtggt ctgccgatgg gccgtatcgt cgaaatctac ggaccagaat
    37321 cttccggtaa aaccacgctg acgttgcagg tgatcgccgc agcgcagcgc gaaggtaaaa
    37381 cctgtgcgtt tatcgatgct gaacacgcgc tggacccaat ctacgcacgt aaactgggcg
    37441 ttgatatcga caacctgctg tgctcccagc cggataccgg cgagcaggca ctggaaatct
    37501 gtgacgccct ggcgcgttct ggcgcagtag acgttatcgt cgttgactcc gtggcggcac
    37561 tgacgccgaa agcggaaatc gaaggcgaaa tcggcgactc tcacatgggc cttgcggcac
    37621 gtatgatgag ccaggcgatg cgtaagctgg cgggtaacct gaagcagtcc aacacgctgc
    37681 tgatcttcat caaccagatc cgtatgaaaa ttggtgtaat gttcggtaac ccggaaacca
    37741 ctaccggtgg taacgcgctg aaattctacg cctctgttcg tctcgacatc cgtcgtatcg
    37801 gcgcggtgaa agagggcgaa aacgtggtgg ggagcgaaac ccgtgtgaaa gtggtgaaga
    37861 acaaaatcgc tgcgccgttt aaacaggctg aattccagat cctctacggc gaaggtatca
    37921 acttctacgg cgaactggtt gacctgggcg tgaaagagaa gctgatcgag aaagcaggcg
    37981 cgtggtacag ctacaaaggt gagaagatcg gtcaaggtaa agcgaatgca actgcctggc
    38041 tgaaagataa cccggaaacc gcgaaagaga tcgagaagaa agtacgtgag ttgctgctga
    38101 gcaacccgaa ctcaacgccg gatttctctg tagatgacag cgaaggcgta gcagaaacta
    38161 acgaagattt ttaatcgtct tgtttgatac acaagggtcg catctgcggc ccttttgctt
    38221 ttttaagttg taaggatatg ccatgacaga atcaacgtcc cgtcgcccgg catatgctcg
    38281 cctgttggat cgtgcggtac gcattctggc ggtgcgcgat cacagtgagc aagaattgcg
    38341 acgtaaactc gcggcaccga tcatgggcaa aaatggcccg gaagagattg atgccacggc
    38401 agaagattac gagcgcgtta tcgcctggtg ccatgaacat ggctatctcg atgacagccg
    38461 atttgttgcg cgctttatcg ccagccgtag ccgcaaaggt tatggacctg cgcgtattcg
    38521 tcaggaactg aatcagaaag gtatttcccg cgaagcgaca gaaaaagcga tgcgtgaatg
    38581 tgacatcgac tggtgcgcac tggcgcgcga tcaggcgacg cgaaaatatg gcgaaccttt
    38641 gccaactgtc ttttcagaaa aagttaagat ccagcgtttt ctgctctatc gtggctatct
    38701 gatggaagat atccaggata tttggcgaaa ttttgccgac tgaacgcata cgggatttta
    38761 cttcccagtc aagaaaactt atcttattcc cacttttcag ttaccagccc ggcggttaaa
    38821 acacgctgta gctggtggcg atatttcgtt agcttgattt caggataatt atgagcaaga
    38881 gcaccgctga gatccgtcag gcgtttctcg actttttcca tagtaaggga catcaggtag
    38941 ttgccagcag ctccctggta ccccataacg acccaacttt gttgtttacc aacgccggga
    39001 tgaaccagtt caaggatgtg ttccttgggc tcgacaagcg taattattcc cgcgctacca
    39061 cttcccaacg ctgcgtgcgt gcgggtggta aacacaacga cctggaaaac gtcggttaca
    39121 ccgcgcgtca ccataccttc ttcgaaatgc tgggcaactt cagcttcggc gactatttca
    39181 aacacgatgc cattcagttt gcatgggaac tgctgaccag cgaaaaatgg tttgccctgc
    39241 cgaaagagcg tctgtgggtt accgtctatg aaagcgacga cgaagcctac gaaatctggg
    39301 aaaaagaagt cgggatcccg cgcgaacgta ttattcgcat cggcgataac aaaggtgcgc
    39361 catacgcatc tgacaacttc tggcagatgg gtgacactgg tccgtgcggc ccgtgcaccg
    39421 aaatcttcta cgatcacggc gaccacattt ggggtggccc tccgggaagt ccggaagaag
    39481 acggcgaccg ctacattgag atctggaaca tcgtcttcat gcagttcaac cgccaggcag
    39541 atggcacgat ggaaccgctg ccgaagccgt ctgtagatac cggtatgggt ctggagcgta
    39601 ttgctgcggt gctgcaacac gttaactcta actatgacat cgacctgttc cgtacgctga
    39661 tccaggcggt agcgaaagtc actggcgcga ccgatttgag caacaaatcg ctgcgcgtaa
    39721 tcgctgacca cattcgttct tgtgcgttcc tgatcgcgga tggcgtaatg ccgtccaacg
    39781 aaaaccgtgg ttatgtactg cgtcgtatca ttcgtcgcgc agtgcgtcac ggcaatatgc
    39841 tgggcgcgaa agaaaccttc ttctacaaac tggttggtcc gctgatcgac gtaatgggct
    39901 ctgcgggtga agacctgaaa cgccagcagg cgcaggttga gcaggtgctg aagactgaag
    39961 aagagcagtt tgcccgtact ctggagcgcg gtctggcgct gctggacgaa gagctggcaa
    40021 aactttctgg tgatacgctg gatggtgaaa cggctttccg tctgtacgac acctatggct
    40081 tcccggttga cctgacggct gatgtttgtc gtgagcgcaa catcaaagtt gacgaagctg
    40141 gatttgaagc agcaatggaa gagcagcgtc gtcgtgcgcg cgaagccagc ggctttggtg
    40201 ccgattacaa cgcaatgatc cgtgttaaca gtgcatctga atttaaaggc tatgaccatc
    40261 tggaactgaa cggcaaagtg accgcgctgt ttgttgatgg taaagcggtt gatgccatca
    40321 atgcaggcca ggaagctgtg gtcgtgctgg atcaaacgcc attctatgcg gaatccggcg
    40381 gtcaggttgg tgataaaggc gaactgaaag gcgctaactt ctcctttgcg gtggaagata
    40441 cgcagaaata cggccaggcg attggtcaca tcggtaaact tgctgcgggt tctctgaaag
    40501 tgggcgacgc ggtgcaggct gatgttgatg aagctcgtcg cgcccgtatt cgtctgaatc
    40561 actccgcaac gcacctgatg cacgctgcgc tgcgccaggt tctgggtact catgtatcgc
    40621 agaaaggttc actggttaac gacaaagtgc tgcgcttcga cttctcacac aacgaagcga
    40681 tgaaaccaga agagattcgt gcggtcgaag acctggtgaa cgcacagatt cgccgtaact
    40741 tgccgattga aaccaacatc atggatctcg aagcggcgaa agcgaaaggt gcgatggcgc
    40801 tgttcggcga gaagtatgat gaacgtgtac gtgtgctgag catgggcgat ttctccaccg
    40861 agctgtgtgg cggtactcac gccagtcgca ctggtgatat tggtctgttc cgcatcatct
    40921 ctgaatcggg tactgctgca ggcgttcgtc gtatcgaagc ggtaaccgga gaaggcgcta
    40981 tcgccaccgt tcatgcagac agcgatcgct taaacgaagt cgcgcatctg ctgaaaggcg
    41041 atagcaataa tctggctgat aaagtgcgct cagtactgga acgtacgcgt cagctggaaa
    41101 aagagttaca acagcttaaa gaacaagctg ccgcacagga gagcgcaaat ctttccagta
    41161 aggcaattga tgttaatggt gttaagctgt tggttagcga gcttagcggt gttgagccga
    41221 aaatgttgcg taccatggtt gacgatttaa aaaatcagct ggggtcgaca attatcgtgc
    41281 tggcaacggt agccgaaggt aaggtttctc tgattgcagg cgtatctaag gacgtcacag
    41341 atcgtgtgaa agcaggggaa ctgattggta tggtcgctca gcaggtgggc ggcaagggtg
    41401 gtggacgtcc tgacatggcg caagccggtg gtacggatgc tgcggcctta cctgcagcgt
    41461 tagccagtgt gaaaggctgg gtcagcgcga aattgcaata atataagcgt caggcaatgc
    41521 cgtggactcg cttcacggca ttcgcattaa cgctatcgac aacgataaag tcaggttgaa
    41581 gttgtgtata tcggctaaac ttaggtttaa cagaatgtaa tgccatgact gcttagatgt
    41641 aatgtgtttg tcattgctta ctttttggcg ttatatgatg gataatgccg ggatacagag
    41701 agacccgact cttttaatct ttcaaggagc aaagaatgct gattctgact cgtcgagttg
    41761 gtgagaccct catgattggg gatgaggtca ccgtgacagt tttaggggta aagggcaacc
    41821 aggtacgtat tggcgtaaat gccccgaagg aagtttctgt tcaccgtgaa gagatctacc
    41881 agcgtatcca ggctgaaaaa tcccagcagt ccagttacta atctttccgc gtctcatctt
    41941 tatcggtgag acgcaccctc aaaatttctt cttcactcca ttgtcttttc gctttactcc
    42001 cgttcattca acttaagtct ccattttttt gcattactgc tatctgacag acctccgttt
    42061 ttctgttgat aaaacactct ttttgacgtt tttacagact aattgagcgt gaagtgtgca
    42121 aacgataaaa gtgtgggaaa aattgtttga cttataagtc tcagaaagta atatgtgcgc
    42181 cacgcagcga cgatgagcga taaacaagtt cttcgaagca ctcgtaagag gcgtgtggtg
    42241 aggtggccga gaggctgaag gcgctcccct gctaagggag tatgcggtca aaagctgcat
    42301 ccggggttcg aatccccgcc tcaccgccat ttgcatccgt agctcagctg gatagagtac
    42361 tcggctacga accgagcggt cggaggttcg aatcctcccg gatgcaccat attctacgta
    42421 ccttcagcga tgaaggtatg gaagaggtgg cggtaataac cgcaggcacc agggaggata
    42481 acgttgcttt agcaacggcc cgaagggcga gccgcaaggc gagtaatcct cccggatgca
    42541 ccatctctta cttgatacgg ctttagtagc ggtatcaaaa aatctgcagt aaagtaagtt
    42601 tcccgatgca tccgtagctc agctggatag agtactcggc tacgaaccga gcggtcggag
    42661 gttcgaatcc tcccggatgc accatctctt acttgatatg gctttagtag cggtatcaat
    42721 atcagcagta aaataaattt cccgatgcat ccgtagctca gctggataga gtactcggct
    42781 acgaaccgag cggtcggagg ttcgaatcct cccggatgca ccatctctta cttgatatgg
    42841 ctttagtagc gatatcaata tcagcagtaa aataaatttt cccaatgcat ccgtagctca
    42901 gctggataga gtactcggct acgaaccgag cggtcggagg ttcgaatcct cccggatgca
    42961 ccatattctc cgtaaccttc agcaatgaag gtatcgaaga ggtagcggta ttaaccgcag
    43021 gcaccaggga ggataacgtt gcgccagcag cggcccgaag ggcgaacgcc agtgagtcat
    43081 cctcccggat gcaccatctt ctccaaaaaa tttccttcaa aaatatctca tttctaaata
    43141 actactttat ccatttcctg aatattcttc tccttctcca tcagcgacaa aatcaagcaa
    43201 ttacgctaaa gtagcgtcat atttttcgac ttgcgagaag accatgtacg agcgttatgc
    43261 aggtttaatt tttgatatgg atggcacaat cctggatacg gagcctacgc accgtaaagc
    43321 gtggcgcgaa gtattagggc actacggtct tcagtacgat gttcaggcga tgattgcgct
    43381 taatggctcg cccacctggc gtattgctca ggcgattatt gagctgaatc aggccgatct
    43441 cgacccgcat gcgttagcgc gtgaaaaaac agaagcagta agaagtatgc tgctggatag
    43501 tgtcgagccg cttcctcttg ttgaagtggt gaaaagctgg catggtcgtc gcccaatggc
    43561 tgtaggaacg gggagtgaaa gcgccatcgc tgaggcattg ctggcacacc tgggattacg
    43621 ccgttatttt gacgccgtcg tcgctgccga tcacgtcaaa caccataaac ccgcgccaga
    43681 cacatttttg ttgtgcgcgc agcgtatggg cgtgcaaccg acgcagtgtg tggtcttcga
    43741 agatgccgat ttcggtattc aggcggcccg tgcagcaggc atggacgccg tggatgttcg
    43801 cttgctgtga gtgaagcgtt atcgcttttc tcgttgtttg ccagtagttt tctcagcgct
    43861 acactattac ccggcaactc cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt
    43921 catccctggg ttttagtttt aacagcaaca atgggtaata gccttggagg gttaactaac
    43981 gttatccttg ggcgtttctt tccattgcgt aaaacatcgc gctggcaaga gaaagctacc
    44041 ggctggctga aacgctatgg tgcagtcacg ctattattaa gctggatgcc cgtggttggc
    44101 gatttactgt gtctgttagc gggatggatg cgcatctcgt ggggaccggt aatctttttt
    44161 ttgtgccttg gtaaagcgtt acgctatgtt gcggttgcag cagctaccgt tcagggcatg
    44221 atgtggtggc actaattgta ggcctgcaca tatggtcacc attacagtta tgctaattaa
    44281 aacgattttg acaggcggga ggtcaatttg atcccggacg tatcacaggc gctggcctgg
    44341 ctggaaaaac atcctcaggc gttaaagggg atacagcgtg ggctggagcg cgaaactttg
    44401 cgtgttaatg ctgatggcac actggcaaca acaggtcatc ctgaagcatt aggttctgca
    44461 ctgacccata aatggattac taccgatttt gcggaagcat tgctggaatt tattacacca
    44521 gtggatggtg atattgaaca tatgctgacc tttatgcgcg atctgcatcg ctatacggcg
    44581 cgcaatatgg gcgatgagcg gatgtggccg ttaagtatgc catgctacat cgcagaaggt
    44641 caggacatcg aactggcaca gtacggcact tctaacaccg gtcgctttaa aacgctgtac
    44701 cgtgaagggc tgaaaaatcg ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactat
    44761 aatttctctt tgccaatggc attctggcaa gcgaagtgcg gtgatatctc gggcgctgat
    44821 gccaaagaga aaatttctgc gggctatttc cgcgttatcc gcaattacta tcgtttcggt
    44881 tgggtcattc cttatctgtt tggtgcgtct ccagcgattt gttcttcttt cttgcaagga
    44941 aaaccaacgt cgctgccgtt tgagaaaacc gagtgcggta tgtattacct gccgtatgcg
    45001 acctctcttc gtttgagcga tctcggctat accaataaat cgcaaagcaa tcttggtatc
    45061 accttcaatg atctctacga gtacgtggcg ggccttaaac aggcaatcaa aacgccatcg
    45121 gaagagtacg cgaagattgg tattgagaaa gacggtaagc ggctgcaaat caacagcaat
    45181 gtgctgcaga ttgaaaacga actgtacgcg ccgattcgtc caaaacgcgt tacccgcagt
    45241 ggtgagtcgc cttctgatgc gctgttacgt ggcggcattg aatatattga agtccgctcg
    45301 ctggatatca acccgttctc gccgattggt gtagatgaac aacaggtgcg attcctcgac
    45361 ctgtttatgg tctggtgtgc gctggctgac gcaccggaaa tgagcagtag cgaacttgcc
    45421 tgtacacgcg ttaactggaa ccgggtgatc ctcgaaggtc gcaaaccggg cctgacgctg
    45481 ggtatcggct gcgaaactgc gcagttcccg ttaccgcagg tgggtaaaga tctgttccgc
    45541 gatctgaaac gcgtcgcgca aacgctggat agcattaacg gcggcgaagc gtatcagaaa
    45601 gtgtgtgatg aactggtcgc ctgcttcgat aatcccgatc tgactttctc tgcccgtatc
    45661 ttaaggtcta tgattgatac tggtattggc ggaacaggca aagcgtttgc agaagcctac
    45721 cgtaatctgc tgcgtgaaga gccgttggaa attctgcgcg aagaggattt tatagccgag
    45781 cgcgaggcgt ctgaacgccg tcagcaggaa atggaagccg ctgataccga accgtttgcg
    45841 gtgtggctgg aaaagcacgc ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat
    45901 ttcatctctg aattcaggga tgatgataac aaatgcgcgt ctttcatata ctcagactcg
    45961 cctgggaaga aagagttcag aaaattttaa aaaaaattac cggaggtggc taaatgccgt
    46021 tgttagatag cttcacagtc gatcataccc ggatggaagc gcctgcagtt cgggtggcga
    46081 aaacaatgaa caccccgcat ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc
    46141 cgaacaaaga agtgatgcca gaaagaggga tccataccct ggagcacctg tttgctggtt
    46201 ttatgcgtaa ccatcttaac ggtaatggtg tagagattat cgatatctcg ccaatgggct
    46261 gccgcaccgg tttttatatg agtctgattg gtacgccaga tgagcagcgt gttgctgatg
    46321 cctggaaagc ggcaatggaa gacgtgctga aagtgcagga tcagaatcag atcccggaac
    46381 tgaacgtcta ccagtgtggc acttaccaga tgcactcgtt gcaggaagcg caggatattg
    46441 cgcgtagcat tctggaacgt gacgtgcgca tcaacagcaa cgaagaactg gcgctgccga
    46501 aagagaagtt gcaggaactg cacatttagt cagtttaagc agaagaggcc gggctggtga
    46561 aaacaccgcc cggcttttgt tacctgtcag tcatccgata ttccacgcta tgtgcttcat
    46621 caggttgagg aacttccagc cttgaggcca tccgcatcac gaccgcttca ggaactgcat
    46681 actcgcgtct ggcattttgc tgcttccatt gcgcccatgg cacttccaga taaacgattt
    46741 ttactcgtgc gccgtaagcc gtaaacagac tgatgagctg gctgcgcagc tggcgggtaa
    46801 tgttcgttgc attccagatg aacggctttt tctggcgtaa aaaaacgcgc gcctcttctt
    46861 ttgcctgttg cacaatacgc ccggtggcgg ttttgtcgtc cggactggca ttgattcgcc
    46921 gccgcatatc atcaaggctg ataacgtcca ttccctggca ctgttcactg atatagcgat
    46981 ctttgcccat acccggtaag ccacacagca aaataacctc aaaaggttca gcctcccagg
    47041 gaacaaaatc aggagaactt tgctcatgag tcaggtagtg ccagcgtgcg gaatcagaaa
    47101 cgaacgggcg catttttccc cagcattgtt gctcatggca aaacagctcg aacagatcga
    47161 tgcgttccag cattgattgc tgatccgggc tttggcgacc aagcagatcg gcacgagcaa
    47221 gcaaagcgag cagacgcgtg tcgatgcgca ttgcagccgt gagcagcaaa cgctccggtt
    47281 ccggacgttc cagtaaccat agcggtaacc catgcagacg caccagcgca acaatttgct
    47341 cgcgtagcac gaacggcgtt gggatatcgc gccataaaat ttgtcgggcc gtcagttcgc
    47401 cccgacgagc atggccaggc gactgaatac gtccgttttc ctgcacggta gtgctgcgct
    47461 tttcaacatc atgcaataac gccgccgccc ataaaacttc ctgctgttga gcggggagtt
    47521 gctggaattc tggctgagta acgagggcgt tcagcaccat ttcggtatgc acgccgacgt
    47581 caccttcacc atgatgctcc gggtcttgtg gcgtgtgatg catctcctca acccagctaa
    47641 aacgctgacg tagcgccgac cagcgtttgt cgtcggtaag ctgccaaatc atgactgttc
    47701 tccataatcg aactcatggg ccatccgcgc ccgttgccag tgtcgtttcc agtggacggt
    47761 ggttttgaca tgatttttgc gcacatactt gaagacgtta tgcgaaaagt cagcgacgga
    47821 aaacgcatca cgattcctgc tgacaatgcc ttccagcgta cagggttgcc cggtttgcgt
    47881 atcccaggga tcgaacgcgc cgcgcgcatt ggtcagcgcc agaaaatcac gttgccagct
    47941 tttttcatca ttccccggtt gcgggccgga gatttcggga acgcagggga aatcaaacag
    48001 agcggcgtaa aactgcacct cttcccagct cagccacata tcctggcagc gaacggcaaa
    48061 cagataaaaa tcctgctcca gggcgcgata ttcaatcgaa tgcacggcat aaagattttc
    48121 accaaacaac tccagatcgc cgagatcgtt tttcagcagt tgccagcgtt ggcgaatttt
    48181 ataggtccat gccgactgcg taggcgcggc atgggaacgg gcaaacacgc cgtagcggtt
    48241 aaggcagtta ttttcaccgt cgagtttttc tgtgtgcacc aattgcgtga tggcctgtaa
    48301 atcctgccag taatcggcat taatgcggtc atcgctggtg gtgccggggg agaaaggata
    48361 atgccaggtt ctcccgtatt ttcgttgggt attcataata atccattatt ttgtgtgtaa
    48421 taaatgtacg cccgcgaggg tgcgagttat ggggttaaaa gaaaaatgtg agtatcaccc
    48481 gcgaaagcga gggcgaaaga aaaagagaat taacaacctg agcgaatgct cagaaccggt
    48541 taatggtgaa tggaagagtt tggggcggcg aaaagataca ccgaatgagt ggataaaaca
    48601 accgtttgca aaatgagcaa ataaaaaggg agccgcagct ccctttgaca accttagtgc
    48661 gcaccgcctc cgccgccacc tgcaccaaat ggcggtttag caaaccacac cagccccagc
    48721 aggacgagga atattccggc tgacatccag aagatctcat tggcggaaat aatcagcccc
    48781 tgattagtga tctgctgggc aatccagcct gatgcctgtt gttgcgtcat ccccagccct
    48841 tccagttggc tgtacatcgc ctgagcgttc gggttaaacg ggtttaccga ctcggttaac
    48901 tgtgcgtggt gcatcgactc gcggttagtc cacatggtgg tggtgatgga tgtgccgata
    48961 gaccccgcca gcgttcgcgt aaagttggag aggctcgatg ccgccgccag tcgttccggt
    49021 ggcaaaccgg acagcgtaat ggtggtcagc ggcataaaga agcaggccac cgcaaacccc
    49081 tggataaact gcggccaagc cgacgcgcca aaatccatac ctggttcaaa ggtataggcg
    49141 cgccagtaga agcagacggc atacataata aagctgaagg ttaccagccg ccgcatatcc
    49201 agtttatgcg cgaagcggcc gataatcggc gacaggatca ccggaataat ccctaccggc
    49261 gcagaggcca gacctgccca cgttgccgtg taaccgtaga cctcctgcaa caactgcggc
    49321 agcagaacaa tcgcgccgaa gtagagcata tacgcgaggc tgatacacaa gcagccgatg
    49381 gtgaagttgc gcgacttaaa caacgacaga tcgactatcg ggttatcgtc ggtcagctcc
    49441 cagacaatca ggaagcagat agccaccact gccaccacgg taaggatgat aatttcctgt
    49501 gatgagaacc agtccagctc tttaccgcgg tcgagcataa tctgcaggct accgatacca
    49561 ataaccagca gtgccagccc cacggcatca atccgccgcc gttcggtgcg ggtttcacgt
    49621 ccgcgcaggg tttgcagtgt catcaacacc accgccacgc caatcggtac gttgatgaag
    49681 aatatccagc cccagtggta attgtcactg atatacccgc cgaggatcgg gccgcaaatt
    49741 ggcgcgacaa tcaccgtcat cgaccacaac gccagcgcga tcgagcgttt ggctggcggg
    49801 tagttattaa gcaatagact ttgcgaaagc gggatcagcg gcccggcgac aatcccctga
    49861 atcacgcgga agaagatcag catattcagg ctgctggaga caccacacgc ccacgacgca
    49921 atagcaaagg cgatggtgga ccagaggaac agtttcactt ccccgacgcg ctttgccagc
    49981 cagccggtaa gcgggatcga gatggcattc gccaccccga aagaagtgat tacccacgtt
    50041 ccctggctga gcgatgagcc cagattcccg gcgatagtgg ggatcgccac gttagcaatg
    50101 gtggagtcca gcacctgcat gaatgtcgcc agtgacagcg caatcgtcat aatgaccagt
    50161 tgcgcgcctt ccagcggttt ttgctgttgc atcacacgca cctctggatt agccagcgtt
    50221 agcttttacg atatcgtcga tcagtttatt gacaggtgcc aggctgattt cacgcgcggt
    50281 gcttaccgct accggtgtgg aacgtacttt atttgccagt acctgaccgt cacggttagt
    50341 ggtattgacg ctcaccagcg tggacaaacc gatacgcagc gggtattgct cgagctgttt
    50401 ctggtccagt tcaatacgaa caggcagacg ctgaacgact ttgatccagt taccggttgc
    50461 attttgcgcc ggaagcagtg agaacgcgct acctgtgccc atatccagac caaccacttt
    50521 tccggtgtat ttcacatcat cgccgtaaat atcggtggtg atggtgaccg gctgaccgat
    50581 acgcatattg gcaatctgcg tctctttaaa gttggcatcc acccacatat tggtggctgg
    50641 aacgaccgcc atcagcggcg tcgttgggct aatttgcgcc ccaggctgta ccgcgcggcg
    50701 ggagacataa ccggtcatcg gactgacaat acgggtacgc tccagcgcca gccaggcgtt
    50761 acgtacttcg gtggcagcct gttgcacggc aggctgatct tccagtttag tccccagaat
    50821 catcgcctga ttggcattgt attgttgaat agcgacgtcc agttgtgcct gggcactggt
    50881 aacggcgtcg cgggcgtgtt gcagctcttc gcgaccaatc aggttggcat tgcccagcgg
    50941 cacacggcgg ttgtagtcgc tttgtgcttt cgcgagggcg attttttgca cctcaatatt
    51001 cgcctgcaac tgtttgctgt taatcatcag ctggtgggtt tggcgaacgc tggaagccag
    51061 tgcagttttg gctttttcaa acgcctggcg agcatctgtc gggtcgagag tgaccagcac
    51121 atcgccttct tttacaaaat cggtgttatc ggcccagact ttcgtcacgc tgccagacac
    51181 ctgagacata atttgaatct gattccctgc cacgtatgcg tcatcagttt cttcgaaatg
    51241 acgcagtacc aaaaaccaat aaatccctat cgctacggca ataattataa agagcaaggt
    51301 gagaaggagg agcagacgct tacgtttgcc gctcttcttt accggttgct gcggggtttg
    51361 agtctccgca tttgcgctca tattgttctc cacgatcttc ttaactcatc ggctgagccg
    51421 acctgcttgc caaaaaggcc agcatgttgc gatgctggcc agtcattttt tcttttataa
    51481 atctggattt ttgagcgaga tgacgcgtta gctcatcgct tcgagaacca caccgtcttg
    51541 ttccatctgg tcgagacggg agagcaattt gcgggtgatt tgctcgagct gatctttttc
    51601 tgttgtgctg agcgcggacc agagttgatg caggcagtta tgctgcggtg gtaaaacctc
    51661 gcgcaaaaac tcgtgacctt tttccgttaa ttgcagatgc aggcagcggc gatcgttatc
    51721 gctttcacga cgttcgatcc aaccgcgttt ttccagttca tcggcaatac gcgtcgcgtt
    51781 ggtacgggat gatccaagag cacaactcaa ttcagaaggc tgaatactgt ggttttcctg
    51841 agactccagc gtaatcaacg ccataaacaa cgtctcgtta atcccctgag ccttcagcat
    51901 tttattgcgg ttctccagca gcttgctttg catgtgcatg caaagacggg tcagaaggat
    51961 ctcctgataa ggaaaatctt cgtggcggct ggcgcgaaat tttagcattt gttcaatggg
    52021 cgtaaacgaa ctatccattt gggtatgacc tcattaattt cagccgatat agtaacgaca
    52081 gtgacaaata aagtaaatgt attgttttag aaaaatgatt cttgtgggtt atataatcgc
    52141 catcactttc caggcgagcc cataggccag cgcactaagc agtgttggga tgataatgct
    52201 gcgtgtttta tagaaactgg cacccagtac cgcgaagccg accagagtgg gcacgaaacg
    52261 gcgtgtatcg tgcatcactt ctggtgcggt agagacaacc agcagagcgc atatcgaggc
    52321 gatgccaatg gtgtcgagca aaatacctac cgcgccacgt ttggttgggc gggcattacc
    52381 cacacgcagg cgcagcggca aatagcggaa gcaataattc gccgcgccaa ccagtaaccc
    52441 aagcagcaga acctcatagc tcatcgggcg ctccttgcca gaatgcctgg attaacgcag
    52501 tgaggcagcc acagacaatg cctgccagaa tggcgacggg aatagaaaat agcgttacgc
    52561 ctgcaagggc accaactaac gctgcggtaa cgcaaagaga ttgtttgcgc tggaaagagg
    52621 cgagcaggaa actcataaag agtgccggaa gcataaaacc taatgctgct tcaacggcgg
    52681 gatatccttg cagcaagccg ctgccggaga atgcccctat taccgtacca aatacccacg
    52741 atgaccatga actgaaggca atgccgatca tccagttctc gctccagcgg cgattattgc
    52801 gtaccagttt tgcggttgcg gcggcaaaaa cctcatccgt caggccaaac gcccacaggg
    52861 cagttttcga tttttgcaga cgctgaataa tacggctacg cagtgacggg ccatacaaaa
    52921 catggcgaac atccattgcc atgacggtca gtgcagcaac ccacaaacta ctcccggctg
    52981 ccagcatcgc ggtaatgacg aactggctcg cgcctgcata aatgatgcag gagaaaaaaa
    53041 cgctttcgag aggagagaat cccagacggg tcgcattcag accgaacgca aaggccaccg
    53101 gaatataact aataacaatc ggtaaactgt ctttgcatcc ttccatgaag gtcgccgaac
    53161 caggagcagg ctgtggagta gggctttcca tagagtgtac gcttaacgat tgttaatatt
    53221 ataaatagac tgaatgaata tcttaacctt atcagactga tgggcttctt aacaccctta
    53281 taagtgtaaa gccacgaaaa cggttgctga ttgcttaaat tgcggcttcc tgacgatgaa
    53341 aacctcgcca ccagaccagt aagttaacca gggcaatcgt cgcgccagcc agacaaacgc
    53401 cagcccaacc gccatgttgc cagactgagg ctgaaattag cgaaccggcg gcaccgccaa
    53461 tgaagtagct ggtcatgtaa cctgcggtca ggcgattacg cgcatcaggg tgtattcgat
    53521 aaattaccgt ctggttagtg atatgcacgc cctgcacggt gagatccagc accaggatgc
    53581 cgataatcag cgccagtacg gaagtgtgac caaaccagat cgccagccat ggaagtaaca
    53641 gcagcagcag accgaaagtt gtggtgtggt gcgatttgcc cttatcggca aatccgcccg
    53701 ccggacgagc gcccaacgct ccggctgctc ccgcaagtcc aaacagacca atgacgccat
    53761 cgctgtaatt aaaaggcgga gcggcgagca aaaaggccat cgaggtccag agaatgctga
    53821 agttggcaaa ggtcaggcag cccagcaacg tgcgggtgcg cagaatttta tcgctgataa
    53881 acatgctgaa aacggaacca agcaactgtg ggtagttgag gtgggtttct gatttcattt
    53941 gtggcagacc acgccataat gccagcgcca tcagtgccat taacaccgaa gcaacccaaa
    54001 agacggtgcg ccagccgccg aggtttgcca gcaacccggc aacggttcgt gccagcaaga
    54061 tccccaacag cagcccgctc ataatggtgc cgaccacttt gccgcgtttg tccggtgaag
    54121 ccagcgtcgc tgccagcgga accagaattt gtgccacgac cgagaataaa ccggttaatg
    54181 ccgtaccgag gatcatcatc gccagcgact gactgctggc agtgattaac atgccgcatg
    54241 ccgccagtaa ggtcatcgag acaatcaggc ggcggcgttc aaacatatca ccgaggggaa
    54301 caagaaacag cagacctgcg gcatagccca actgcgcggc ggtaacaata aagcctgccg
    54361 aactggcgga aagggaaaag ttacgcgcga tggtgtcgag caatggctgg gcgtaatagt
    54421 tactggctac cgccaggccg gtggcgatag acatcagcac gatcagcgcc gggctaagct
    54481 catgattagg tttagtcatt atttttgttc atcaggtgat gattaagctg gaaatcaata
    54541 atagcggatg ttgatgaata tgggatgaaa gatagattgt tgaaatgtgc ggtgtggttt
    54601 gtaggtatga taagacgcac cagcgtcgca tcaggcattg tgtacagagc ggcggatgag
    54661 gcaaaaacgc cttatccgcc cgaataaaaa ttacttctgc gctgccagcg cctcattcac
    54721 ccagccatcg aactgctgct ggtgggcttt gatccagcca tcaacgtggc cctgaatatc
    54781 gccttctgag gctttgccgt catgcataat ggcgttctgg gcgttaatat cagccactgg
    54841 taactgcata atggcaaaca gtttcgctgc tgccgggttt ttctcggccc aggctttgtt
    54901 ggcaacgata tgcatggtac tgaccgggaa gccgtaattc gcgccattcg gcagtttggt
    54961 atcggcattt ttatcgcccg gcagtgcgga gaacggcacc tgcaaccaga ccacatcttt
    55021 ccccggtttc agttcgttac tcacccagta cggcgtccag gtgtagtaaa acaccggctt
    55081 accctctttg taacgactga tggtgtcagc catcatcgct gcgtagttcc cctgattatg
    55141 cgtcacggta ttggtcagtc catacgcggc aagctggtgg ttgatcgcac cttcgcagcc
    55201 ccagcctggg ttacagccgg ttaaatccgc tttaccgtcg ccgttggtat cgaacagttt
    55261 ggcgatcttc ggatctttaa gttgcgcgat gttggtaatt ttgtactggt cggcagtttt
    55321 cttatcgatc aggtaaccct gtgccgcgcc gttaacaaat actccttcac gataaaattt
    55381 cttatcgcca ccggcagctt cgtacatgtt gtcatgcagt ggcgtccagt tcactgcggt
    55441 gaaggttgca tcgccggaag caagcgaggt gtagccaacg ttgtaatcca cttcgctggg
    55501 tttattgacg gtataaccta atttctccag cgcgcggctg accagcagcg tctggaaagt
    55561 ttcttcggtg atggtgctct gaaccggatt aacagtaatg cctttgcccg gcaggtcggc
    55621 agcaaaagtt tgtgtagaga taagcgtggc aaacgctgtc gcaaaaagta cgctatgtcg
    55681 cattgttatt cctttttttg gcagggtgat aatgcccggc gtcaccgggc aagtgcagag
    55741 ttacttaatg aatgggcggg tcagcagacc gacagggcca gtggtgtacc agcgacggtt
    55801 gccgcgactg cgtgagtcgc gcccaacggc ctgcgtcagg cggtcgagga taatggcgag
    55861 gatcacaatc ccgacgccgc caacggtggc aagccccata tccagacgac cgataccgcg
    55921 aagcaccatc tgacccagcc cgccgacggc aatcatcgag gcgatgacca ccatagaaag
    55981 ggccagcatc agcgtctggt taacgcccgc cataatggtc ggcatcgcca gcggtaactg
    56041 aactttgaac agcatctggc gcgggctggc accgaatgag cgcgaggctt caatcagatc
    56101 cgccggaacc tggttaattc ccagaatcgt cagacggata atcggcggca gcgcaaagat
    56161 gatcgtcacc accacgcccg gcacgttacc gataccaaac agcatgacga ttggtaccag
    56221 ataaacaaac gcgggcgtgg tctgcatggc atcaagcagt ggacgaataa ttttcgccgc
    56281 tcgcggactt ctcgccagcc agatccccaa cggcaaaccg atgacgatac agaacagcag
    56341 ggcggttagc accagcgcca gagtaaccat tgcctgtgac caggcaccga ttgcgccgat
    56401 ggcaatcagc gaaaccagtg tcgccacgcc cattccgaca ccggaaattt gccaggcgat
    56461 gagagcgaaa acgataatcg ccaccggagc gggcataccc agcagcaatt gctggaaacc
    56521 gttgaggatg taatcaaccg gaacgcgcac gccctgaaag acgggacgaa aatgggtaac
    56581 gacccagtcg atcccttcag tgacccaact gtcgagcggg atcagcgttt tatggaacgg
    56641 atcgagaata ttaaaatgct cgacgtttgg cgcaggcgta ctggtcagcc agtcagcacc
    56701 accgccgtca gtcggtgcag cagccggtgt accccaggcg tctgcggact gtgcggcact
    56761 gtccgccgct ggcgtggtat cccacggatt attttgatca gccattattt accccctcac
    56821 gatctaaagc gcgcagcagc attcctttcg aaatgatgcc gacatactgt tggtcctcat
    56881 cgaccacggg caccgcacag ggggcctgtc cgacatgaga gagcaactcg ctaagaggcg
    56941 tttgtgcatc gaccgctaac ggcgcatcaa tcagcgccgc atcaagacct tgctgctgcg
    57001 ttaacgcggc tttaagcgaa tcgatagaca cagcgccgac aaacttatta ccgcgttcga
    57061 tgacgtagcc atattcgcga tcttcatcct gcaataattt cagtgccgaa cgcgggccga
    57121 agccgggggt tttacgaatt aagccgttcg gtgtacgacg ggcaatatct ttcgcactga
    57181 acacctggct aatatcgacg ccacggaaga aggtacggac ataatcattc gccggattat
    57241 tgagaatttc atccggtgtg ccgacctgta ctacttcacc attttgcata atggcaattc
    57301 ggtcgccaat acgcatggct tcatcgagat cgtgggaaat aaagacaatg gtgcgctgat
    57361 gtttcgcctg taattttacc agctcatcct gcatctcggt gcgaattaat ggatcgagcg
    57421 ccgagaaggc ttcgtccatt aataatatat ccggattaat cgctaacgcg cgggctaatc
    57481 ccacacgttg acgcatcccg ccagagagtt catccgggta gctgtgggca taattttcca
    57541 gcccgacctg acgcagtgca tcaagggctt tttcacggcg ttcttcggca ttaattccgg
    57601 ccagttccat accgaacgca gtattgtcca gcacggtcat atgcggcatt aaggcaaagg
    57661 actggaagac catcgcaatc ttttttctgc gcacctcacg gagttcggcg tcggatattt
    57721 tggcaatatc cacaccatca atcagcactt gcccgcgggt gggttcaatc aggcgattga
    57781 gaaggcgtac cattgtggat ttacccgaac cggataatcc catgatgaca aatatctcgc
    57841 cttcttcaat ggccagactg gcgtctttaa cgccaagcga tagcccagtt ttttccagaa
    57901 tttgttcttt tgaaagtcct tgttcgatat atttgaaagc tcg
//
LOCUS       DAZKJR010000012        53061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKJR010000012 DAZKJR010000000
VERSION     DAZKJR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.012"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..507)
                     /gene="curA"
                     /locus_tag="ACHOWS_000755"
     CDS             complement(<1..507)
                     /gene="curA"
                     /locus_tag="ACHOWS_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFQ0046387.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQI"
     gene            688..1203
                     /gene="mddA"
                     /locus_tag="ACHOWS_000756"
     CDS             688..1203
                     /gene="mddA"
                     /locus_tag="ACHOWS_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFQ0046388.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAI"
     gene            1203..1652
                     /gene="ydcZ"
                     /locus_tag="ACHOWS_000757"
     CDS             1203..1652
                     /gene="ydcZ"
                     /locus_tag="ACHOWS_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFQ0046389.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(1653..1886)
                     /gene="ydcY"
                     /locus_tag="ACHOWS_000758"
     CDS             complement(1653..1886)
                     /gene="ydcY"
                     /locus_tag="ACHOWS_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFQ0046390.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1972..2145)
                     /gene="ortT"
                     /locus_tag="ACHOWS_000759"
     CDS             complement(1972..2145)
                     /gene="ortT"
                     /locus_tag="ACHOWS_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFQ0046391.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2340..2435
                     /gene="yncL"
                     /locus_tag="ACHOWS_000760"
     CDS             2340..2435
                     /gene="yncL"
                     /locus_tag="ACHOWS_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFQ0046392.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2532..3956)
                     /gene="patD"
                     /locus_tag="ACHOWS_000761"
     CDS             complement(2532..3956)
                     /gene="patD"
                     /locus_tag="ACHOWS_000761"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFQ0046393.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(3978..4772)
                     /gene="ydcV"
                     /locus_tag="ACHOWS_000762"
     CDS             complement(3978..4772)
                     /gene="ydcV"
                     /locus_tag="ACHOWS_000762"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0046394.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAVYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4762..5703)
                     /gene="ydcU"
                     /locus_tag="ACHOWS_000763"
     CDS             complement(4762..5703)
                     /gene="ydcU"
                     /locus_tag="ACHOWS_000763"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0046395.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWGNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(5704..6717)
                     /gene="ydcT"
                     /locus_tag="ACHOWS_000764"
     CDS             complement(5704..6717)
                     /gene="ydcT"
                     /locus_tag="ACHOWS_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0046396.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKSASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVDKKQRYAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDALMADKLCGMTGSFALRPEHIRFNIPGEVQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(6735..7880)
                     /gene="ydcS"
                     /locus_tag="ACHOWS_000765"
     CDS             complement(6735..7880)
                     /gene="ydcS"
                     /locus_tag="ACHOWS_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFQ0046397.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGIRDPYQLTEEQY
                     QAVLKVMRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMSWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(8125..9531)
                     /gene="ydcR"
                     /locus_tag="ACHOWS_000766"
     CDS             complement(8125..9531)
                     /gene="ydcR"
                     /locus_tag="ACHOWS_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0046398.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(9610..10026)
                     /gene="hicB"
                     /locus_tag="ACHOWS_000767"
     CDS             complement(9610..10026)
                     /gene="hicB"
                     /locus_tag="ACHOWS_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFQ0046399.1"
                     /translation="MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(10070..10246)
                     /gene="hicA"
                     /locus_tag="ACHOWS_000768"
     CDS             complement(10070..10246)
                     /gene="hicA"
                     /locus_tag="ACHOWS_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFQ0046400.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLN"
     gene            10468..10698
                     /gene="yncJ"
                     /locus_tag="ACHOWS_000769"
     CDS             10468..10698
                     /gene="yncJ"
                     /locus_tag="ACHOWS_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFQ0046401.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGHR"
     gene            complement(10790..12751)
                     /gene="rlhA"
                     /locus_tag="ACHOWS_000770"
     CDS             complement(10790..12751)
                     /gene="rlhA"
                     /locus_tag="ACHOWS_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFQ0046402.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(12824..13360)
                     /gene="sutR"
                     /locus_tag="ACHOWS_000771"
     CDS             complement(12824..13360)
                     /gene="sutR"
                     /locus_tag="ACHOWS_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFQ0046403.1"
                     /translation="MENLTRFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQVMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            13413..14624
                     /gene="ydcO"
                     /locus_tag="ACHOWS_000772"
     CDS             13413..14624
                     /gene="ydcO"
                     /locus_tag="ACHOWS_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFQ0046404.1"
                     /translation="MLRYLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDDQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGVVIVIAQGDVVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(14659..15327)
                     /gene="ydcL"
                     /locus_tag="ACHOWS_000773"
     CDS             complement(14659..15327)
                     /gene="ydcL"
                     /locus_tag="ACHOWS_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFQ0046405.1"
                     /translation="MRTTSIAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPKPSTQVGQKVLDKILSYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(15666..16259)
                     /gene="tehB"
                     /locus_tag="ACHOWS_000774"
     CDS             complement(15666..16259)
                     /gene="tehB"
                     /locus_tag="ACHOWS_000774"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFQ0046406.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLENLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(16256..17248)
                     /gene="tehA"
                     /locus_tag="ACHOWS_000775"
     CDS             complement(16256..17248)
                     /gene="tehA"
                     /locus_tag="ACHOWS_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFQ0046407.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
     gene            17372..18352
                     /gene="ydcK"
                     /locus_tag="ACHOWS_000776"
     CDS             17372..18352
                     /gene="ydcK"
                     /locus_tag="ACHOWS_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFQ0046408.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDCETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(18344..18883)
                     /gene="rimL"
                     /locus_tag="ACHOWS_000777"
     CDS             complement(18344..18883)
                     /gene="rimL"
                     /locus_tag="ACHOWS_000777"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFQ0046409.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(18946..19170)
                     /gene="ydcH"
                     /locus_tag="ACHOWS_000778"
     CDS             complement(18946..19170)
                     /gene="ydcH"
                     /locus_tag="ACHOWS_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFQ0046410.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            complement(19310..20965)
                     /gene="opgD"
                     /locus_tag="ACHOWS_000779"
                     /gene_synonym="mdoD"
     CDS             complement(19310..20965)
                     /gene="opgD"
                     /locus_tag="ACHOWS_000779"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFQ0046411.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTDFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(21190..22533)
                     /gene="ydcJ"
                     /locus_tag="ACHOWS_000780"
     CDS             complement(21190..22533)
                     /gene="ydcJ"
                     /locus_tag="ACHOWS_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0046412.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            22750..23673
                     /gene="ydcI"
                     /locus_tag="ACHOWS_000781"
     CDS             22750..23673
                     /gene="ydcI"
                     /locus_tag="ACHOWS_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0046413.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(23711..25351)
                     /gene="trg"
                     /locus_tag="ACHOWS_000782"
     CDS             complement(23711..25351)
                     /gene="trg"
                     /locus_tag="ACHOWS_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFQ0046414.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            25750..25899
                     /gene="hokB"
                     /locus_tag="ACHOWS_000783"
     CDS             25750..25899
                     /gene="hokB"
                     /locus_tag="ACHOWS_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFQ0046415.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(25877..25945)
                     /gene="yncP"
                     /locus_tag="ACHOWS_000784"
     CDS             complement(25877..25945)
                     /gene="yncP"
                     /locus_tag="ACHOWS_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFQ0046416.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(25971..26144)
                     /locus_tag="ACHOWS_000785"
     CDS             complement(25971..26144)
                     /locus_tag="ACHOWS_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046417.1"
                     /translation="MKKLALILVIGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCIN"
     gene            complement(26389..26919)
                     /gene="cybB"
                     /locus_tag="ACHOWS_000786"
     CDS             complement(26389..26919)
                     /gene="cybB"
                     /locus_tag="ACHOWS_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFQ0046418.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            27108..28109
                     /gene="gap"
                     /locus_tag="ACHOWS_000787"
     CDS             27108..28109
                     /gene="gap"
                     /locus_tag="ACHOWS_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0046419.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILAKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(28150..29589)
                     /gene="aldA"
                     /locus_tag="ACHOWS_000788"
     CDS             complement(28150..29589)
                     /gene="aldA"
                     /locus_tag="ACHOWS_000788"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFQ0046420.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(29786..30586)
                     /gene="ydcF"
                     /locus_tag="ACHOWS_000789"
     CDS             complement(29786..30586)
                     /gene="ydcF"
                     /locus_tag="ACHOWS_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFQ0046421.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(30858..34760)
                     /gene="hrpA"
                     /locus_tag="ACHOWS_000790"
     CDS             complement(30858..34760)
                     /gene="hrpA"
                     /locus_tag="ACHOWS_000790"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFQ0046422.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELSLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            34961..35566
                     /gene="azoR"
                     /locus_tag="ACHOWS_000791"
     CDS             34961..35566
                     /gene="azoR"
                     /locus_tag="ACHOWS_000791"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFQ0046423.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(35620..36912)
                     /gene="ynbD"
                     /locus_tag="ACHOWS_000792"
                     /pseudo
     CDS             complement(35620..36912)
                     /gene="ynbD"
                     /locus_tag="ACHOWS_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(36926..38683)
                     /gene="ynbC"
                     /locus_tag="ACHOWS_000793"
     CDS             complement(36926..38683)
                     /gene="ynbC"
                     /locus_tag="ACHOWS_000793"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFQ0046424.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLASSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIISDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALVFEKMQ
                     SFISHLYANKSQKLDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIFLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRGN"
     gene            complement(38699..39595)
                     /gene="ynbB"
                     /locus_tag="ACHOWS_000794"
     CDS             complement(38699..39595)
                     /gene="ynbB"
                     /locus_tag="ACHOWS_000794"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0046425.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWVTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGLNC
                     FELFLLFIPLFGFLILATWRVFVGEPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGEALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     VSLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(39595..40200)
                     /gene="ynbA"
                     /locus_tag="ACHOWS_000795"
     CDS             complement(39595..40200)
                     /gene="ynbA"
                     /locus_tag="ACHOWS_000795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0046426.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKRQVSANQITLAALALSLLTGL
                     LLALVPYHGMFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWVL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMNAEK"
     gene            complement(40369..41724)
                     /locus_tag="ACHOWS_000796"
                     /pseudo
     CDS             complement(40369..41724)
                     /locus_tag="ACHOWS_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(41788..42648)
                     /gene="pdxI"
                     /locus_tag="ACHOWS_000797"
     CDS             complement(41788..42648)
                     /gene="pdxI"
                     /locus_tag="ACHOWS_000797"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFQ0046427.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLGALDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(42879..43469)
                     /gene="paaY"
                     /locus_tag="ACHOWS_000798"
     CDS             complement(42879..43469)
                     /gene="paaY"
                     /locus_tag="ACHOWS_000798"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFQ0046428.1"
                     /translation="MPIYQIDGLTQFVPEESFVHPTAVLIGDVILGKGVYVGPNTSLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIHRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRSKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(43451..43603)
                     /locus_tag="ACHOWS_000799"
                     /pseudo
     CDS             complement(43451..43603)
                     /locus_tag="ACHOWS_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
     gene            43604..43810
                     /locus_tag="ACHOWS_000800"
                     /pseudo
     CDS             43604..43810
                     /locus_tag="ACHOWS_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(43982..44308)
                     /gene="ydbL"
                     /locus_tag="ACHOWS_000801"
     CDS             complement(43982..44308)
                     /gene="ydbL"
                     /locus_tag="ACHOWS_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFQ0046429.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(44316..44501)
                     /gene="ynbE"
                     /locus_tag="ACHOWS_000802"
     CDS             complement(44316..44501)
                     /gene="ynbE"
                     /locus_tag="ACHOWS_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFQ0046430.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(44498..47137)
                     /gene="ydbH"
                     /locus_tag="ACHOWS_000803"
     CDS             complement(44498..47137)
                     /gene="ydbH"
                     /locus_tag="ACHOWS_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFQ0046431.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGHLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDSALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
     gene            47345..48334
                     /gene="ldhA"
                     /locus_tag="ACHOWS_000804"
     CDS             47345..48334
                     /gene="ldhA"
                     /locus_tag="ACHOWS_000804"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFQ0046432.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            48445..48867
                     /gene="hslJ"
                     /locus_tag="ACHOWS_000805"
     CDS             48445..48867
                     /gene="hslJ"
                     /locus_tag="ACHOWS_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFQ0046433.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(48864..49130)
                     /gene="ydbJ"
                     /locus_tag="ACHOWS_000806"
     CDS             complement(48864..49130)
                     /gene="ydbJ"
                     /locus_tag="ACHOWS_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFQ0046434.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTTIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            49404..52928
                     /gene="nifJ"
                     /locus_tag="ACHOWS_000807"
     CDS             49404..52928
                     /gene="nifJ"
                     /locus_tag="ACHOWS_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0046435.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPDSVRTVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSVDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLKDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        13514 a        13837 c        12559 g        13151 t
ORIGIN      
        1 gatttgaccc accgtcgccc ccacaggacc tgtcgccgca gcaacaacca atgtttcgcc
       61 ctctttgggc tgaccgatat ccagcagccc catataagcg gtaaagcctg gcatccccag
      121 cacacccagc gaccacgatg gattttgcgg atgatcgcca agtttcacca gatcatcacc
      181 actggatatg tcatagtcct gccatccact gtagcccagc acccagtcgc caggctggta
      241 atcaggatga ttcgactcca caacacggct caccgtaccg ccgaccatta cgccaccaat
      301 atcaaccggc ggtgaataag atggctcatc gctcatacgt ccacgcatat acgggtccag
      361 tgacaaataa actgtgcgca gtaacacctg tccttcaccc ggtgtggcga tatcatcttc
      421 ttcaagacgg aaattctccg gaaccggcgc gccatgcgga cgcgaggcca gaacccaacg
      481 tcgattacgc tgcttttgtt gccccataat gctctcctgt gcttttggca tgaatccatc
      541 aatagtagca ggctcgcagg aatggttttg tttgcagccg tcacatcccc tctgcaacac
      601 gcaataattt catcgaggaa tccgttgctt cgggctgttt tcgatagtat tgataacgtc
      661 atcatcatta tttaatcgag gccaaacatg tccatccgtt ttgcccgcaa agccgactgt
      721 gctgccattg cggaaattta taaccacgcc gtgttgtata cggcggctat ctggaatgac
      781 caaacggtgg atgcagataa ccgcattgcc tggtttgaag cgcggaccat agcaggttat
      841 ccagtgctgg tgagcgaaga agatggcgta gtgacgggat atgcctcgtt tggcgactgg
      901 cgtagtttcg atggttttcg ccataccgtg gaacattcgg tttatgtcca tcccgatcat
      961 cagggcaaag gtctggggcg taaattgtta agccgattga ttgatgaagc gcgggattgc
     1021 gggaagcatg tcatggtcgc cgggatcgaa tcgcaaaatc aggcgtcttt gcatctgcac
     1081 cagtcgctgg gatttgtcgt caccgcgcaa atgccgcagg tgggcactaa atttgggcgc
     1141 tggctggacc tgacatttat gcagttgcaa ctcgacgaac gcagtgaacc ggacgcaatc
     1201 tgatgaatca gtcgctcacc cttgcttttt tgattgccgc cgggattggt ctggtagtcc
     1261 agaacacgct gatggtgcgc atcacccaga cctcctctac cattctcatc gccatgttgc
     1321 tgaactcttt ggtaggaatt gtgctctttg tttctatttt atggtttaaa caaggtatgg
     1381 cggggtttgg cgaactggtg tccaacgtgc gctggtggac acttatacct ggcttgctgg
     1441 gatcgttttt tgtcttcgcc agtatcagcg gatatcagaa tgtgggagcg gcgacaacta
     1501 ttgcggtact ggtcgccagc cagctgattg gcggtctggt gctggatatc ttccgcagcc
     1561 atggcgtacc actacgggcg ttgatcggcc caatttgtgg cgcaatttta cttgtggttg
     1621 gtgcctggct ggtggccagg cgcacatttt aatcagagga tggtgccacc tttggttaac
     1681 tgttcgtggc gcgcttccat atcttcttta tgtttgcgac cgtgatgggc gatcgctgtg
     1741 cgaagacgct gctgctgggt atagcgttct tcccgactta actccgcatc atcgctcagg
     1801 gcgattaata attcgttcat atgggcaatg acgctctctt caatcgcggc atccacgcgc
     1861 gcgatgactt catccagatg cgacatactt tctccttagc cacttgtgaa atttatcgat
     1921 tcatccaaat gatataagtc tgcgtggtgc cgtcaagcca gtgagtaaga tttaaaacag
     1981 tgaaaacaac agcgccaccg ggaaactcat cggccatgtt gcccccacca gaaaggcgct
     2041 taagaaacgg atgcgtttct tatcgtgaga aagaaaccag gtgataagaa atgcgattgc
     2101 tgccataacc gcataaaaaa caagcatgtg ttgatagaga gacatgtacg ggcgtccagc
     2161 cagatgaata aaacgcgcgc aaatatgcgt cattttttga cgtacatcaa gttttttaac
     2221 cggtttgaga aaaaaacatc aaacagcaac ttgcccttat ccatcttcag aatgagtatt
     2281 attgcggatt ttcttaaccc gtactataca cataggggca atgataaaag gtggcaaaaa
     2341 tgaatgtttc cagtagaact gtagtactga taaatgtctt tgctgctgtt ggtttgttta
     2401 ctcttatctc tatgagattt ggctggttta tttgatgtag tgattttgca cgcagcacgt
     2461 actgtcagag aaatcagtgc tctgacagta cgcaagatca tctcatactc ttacgatctg
     2521 caatattatc cttaatgttt aaccatgacg tggcggacga cggtgtaatc ctccagccca
     2581 taaagtgaca tatccttgcc gtaaccagaa agtttctgcc caccgtgcgg catttcactt
     2641 accagcatga aatgggtatt gacccaggta caaccatatt gcagccgtgc gctgacgcga
     2701 tgcgccctgc ccacatcttt cgtccatacc gaagatgcaa gtccgtactg gctgtcattc
     2761 gcccagttta ccacctgttc ttcgttgtcg aagagtgtaa cactcactac tggaccaaat
     2821 acctcttttt gcacgatggc atcgtcctgt aatgcgccag ccagcagcgt cggcgcatag
     2881 taatagccat tacccttgcg cttttcaccg ccagtgatca ctttgatgtg ccctgtcgct
     2941 ttcgcctctt ctactgcctt gctgacgcgt tcgagatgcg ccagcgagct taaaggtcca
     3001 agctccgtag actcgtcatc cggcgcacca gattttaacg ttgccaccgc agcacccagt
     3061 ttttccacca gcgtatcgta aatgcctttt tgcgcgtaga tccgacaagc cgcagtacaa
     3121 tcctgtccgg cattgtaata gccaaatgta cgtacacctt cgaccactgc ctcaatatcc
     3181 gcatcatcaa aaacaatcac tggcgctttg ccaccaagtt ccatatgagt acgctttatg
     3241 gacggcgcgg tatggctgat gatgtgttcc ccagtggcga tagagcccgt cagcgacacc
     3301 atccgcactt tgggatgacc ggtcagcgga tcgcccaccg ttttgcctct gccaaacagt
     3361 acattaatca cgccagccgg gaagatatct ttcgccaact ctgccaactt caacgctgtc
     3421 agcggggtaa tttctgatgg tttaagcact acgcagttcc ctgccgccag cgccggagcc
     3481 agtttccacg cagccatcat cagaggataa ttccacggtg cgatagaagc cacgaccccc
     3541 aacggatcgc gacggatcat cgaagtatga ccttcaagat attcacctgc cgccagacca
     3601 ttcagacagc gcgccgcacc agcgaaaaag cgaaaaacat cgacaatcgc cgggatttca
     3661 tcattgaacg cactatgcag cggtttgcca caattacggg actccagttc ggcaaaaacc
     3721 tgaccatttt cttcgataac atcagccagt ttcagcagac attccgcacg cactttcggc
     3781 gtggtttgcc cccattcggc aaatgctgca tctgccgcgc gaacagcagc atcgacctgc
     3841 tctgcggatg cctcggcaat ctccagtaaa acgtcccccg ttgccggatt atagacaggc
     3901 tgtttttccc cttcgccgct aaccagttct ccgttaatca gtaacttatg ttgcatagca
     3961 tattcctgta tcagtggtta ttgaccattg tcgccttcgc gggttagcca ccaggccccc
     4021 aggatcggca aggttgttac caacataacc agcagtgcca ccacgttagt taccggtaca
     4081 tcacgcggtc gcccaagctg attgagcaac cacaacggta acgttcgttc atgacctgcc
     4141 gtaaaggtcg taacgatgat ttcatcgaac gacaaggcaa acgccagcat tcctcctgcc
     4201 agtaacgccg aactgagatt cggcaacact acgtagcgga aggtttgcca gccattagcc
     4261 ccaagatcca ttgacgcctc aaccagactc cacgaggtgc ggcgaaaacg ggcgatgaca
     4321 ttgttaaaga ccaccactac acaaaaagtc gcatgaccga ccacgatggt gaaaaatccc
     4381 ggctccagat tgatggtttt aaaggcggtt aataacgcca gaccagtgac aatgcccggc
     4441 agcgcaatgg gcagcagcag taacagcgaa atggcgtttt tgccgaaaaa gtctcgtcgc
     4501 cacagcgcgg cagcggccag cgtccctaac accagcgcga ttaatgtcgc cagcgccgcc
     4561 actttaagtg acagtgtcac ggcatcaaga atatcactac gctgtgctgc cacgctaaac
     4621 cagcgcagcg tcaggccttg cggtggaaaa ctaaacgccg catcttcagt gttaaacgca
     4681 taaacggcga taatcagaat gggaaaatgt aagaaaacca cgccgcccca ggccgccagt
     4741 ttgaggaaaa acggtgcgcg ttcagagtgc atcgaacgct cccagacgtt tcacgaacgc
     4801 taggtaaagt gcgatgagta caatcggcac tagggtgaat gccgccgcca tcggcatatt
     4861 gccaatcgcc ccctgctggg aataaaccat attgccgata aaatatcctg gagggccaac
     4921 cagctgcggg acaataaaat cgcccagcgt gagtgagaag gtaaagatag agccagcggc
     4981 aatacccggg attgccagcg gcagcaccac atagcgaaag gtttgtcgtg gacgtgcgcc
     5041 gagatcagct gacgcctgca acaatgacgg cggcaaacgc tcaagcgccg cctgcaccgg
     5101 gaggatcatg aacggcaacc agatatagag aaacaccaga aagcgcccca gcccggaagt
     5161 tgacagcgta ttacctccca ccgccggtag cgttaggaac gcagtcagca gtggttccag
     5221 ccccagatgt tgtaaaaacc actgcgccac gccatctttt gccagcaata acgtccaggc
     5281 ataggctttc acaatgtagc tcgcccacat cggcaacatt accgcgatat aaaaaaacgc
     5341 tttcattttc ccgctggtat agcgcgccat ataccacgcc attggaaaag ccagaatggc
     5401 gctggcgata gtgactgcca cagccataat cagcgtgcgg agaatgatgt cgtaattcgc
     5461 cggattaaac agcgcacgga tattcgccag cgtcagttcc ggcgttaccg acatggtgaa
     5521 atcgtcaaag gtataaaatc cctgccataa cagtgtcagc agcgagccga aatagacaat
     5581 gccaaaccac attagcgggc caagcagcag taaaaacagc cccagccccg gattacccca
     5641 gaagaaaccg gacaccttac ccagacctgg acgtgaaggt gattgcaata cgttcatcgc
     5701 cattcacctc tcctcaacca gcggtaccat cacatcacgc gaccaggaaa ccatcacctg
     5761 ttgtccgggc gtgagcgtgg cgggcagttc ttcgcctgtc atattggcct gactcaccag
     5821 cagtttttca ccgccgttca gtctcagttc aaaacgggtc gccgcgccct gatactgcac
     5881 cgcctggatc gtgccattag cctgcacttc gccggggatg ttgaagcgga tatgttccgg
     5941 tcgcagggcg aagcttcccg tcatgccaca aagtttgtct gccatcagtg catcaaaaac
     6001 attcgatgtc ccgacgaacc cggcaacaaa cggcgtgcgc gggcgcatat agagatcacg
     6061 aggggaatcg acctgctcaa tgcgtccatt attgaaaacc gccacacgat cggacatcga
     6121 taacgcttcg ccctgatcgt gggtaacgaa gataaaagtg ataccgagag actgttgcag
     6181 ttttttcagt tccagctgca tctgctcacg caatttgaga tccagtgcgc cgagcggttc
     6241 atccaacagc aatacgcgcg gttcattcac caatgctctg gcgatagcaa cccgctggcg
     6301 ctgaccacca gaaagctgtg acggcttacg ttgctgaaca aaaccaagcg ccactttctc
     6361 cagcgcttct tgcgccatag catatcgctg ttttttatcc acgcctttga ccatcagccc
     6421 ataggccacg ttgtcaagaa tcgacatatg cggaaaaagt gcgtagtcct ggaagacggt
     6481 attaacatcc cgctcccacg gtggcagatt gctggcggat ttaccaaaga tagagatagc
     6541 cccgccggaa agctgttcga agccagcaat caggcgcagg caggtggttt tgccggagcc
     6601 ggacggcccc agcatagaga agaactcacc atcttttatc gcaatactga cgccatctac
     6661 tgcgcgcacg tcaccgtaca accgtgagac gttgtcaaac tccactgcgt acgtcataaa
     6721 acaccccagc gaagttagcg accgcccata atggcaatgt aatcctgcgt ccagcgactg
     6781 tagggaacaa acttgccccc ttctgctata ggcgttttcc agaaggcgat tttatcgaaa
     6841 tagttaaaac cgttggtttc acaacctttt tcacctaata acggactggc tttacaccct
     6901 tgcggcacta ctggtaacga gccaaaccag gccgccacat caccctgcac ttttggcgtt
     6961 aatgaccagc tcatccattt gtaggcgcaa accggatgtt tcgcttcgct atgtagcatg
     7021 gtggtgtcag cccaaccggt aacgccctcc ttcgggaaaa cggtagcgac aggctggcct
     7081 tcggctttca gggcgttggc ctgatagggc catgcactgg aagcaaccac accctcgttt
     7141 ttgaaatcgc tcatttgcac ggtagtgtca tgccagtagc gatggatcaa actatgttga
     7201 tcgcgcatca ctttcagtac cgcctgatac tgttcttcgg tgagttgata cggatcgcgg
     7261 atgcccaact gcggctgagt ggctttaacg aacaacgcag cgtccgcaat gtagataggg
     7321 ccatcataag cctgaacgcg gcctttattg ctcttgccgt ccggcagatt ctgctcaaca
     7381 aaaaccactt gccagctatc cggcggcgtc gggaaggttt tagtgttgta catcagcagg
     7441 ttcggccccc attggtaagg tgtgccgtaa actttgccgc caacgttaaa ccagtcgcct
     7501 ttaaccacgc gcggatcgag cgctttccag ttgggaatca aggcggtatt aattggctgc
     7561 acgcgtttac ccataatcaa acgcagcgag gcatcgccgg atgccgtaac cagatcgtaa
     7621 ccccctttgg ccatcagact gaccatttca tcggaagtcg cggcggtttt aacattcacc
     7681 gcgcagcctg tctctttttc gaattgcgtt acccagtcgt attgtttatc agtttgtccg
     7741 cgttcgatgt atcccggcca ggcgataata tccagtcgcc cttccggttt atctaaattg
     7801 gtaggtggtt cggcggcgtg agcggtcatt attgtcatgg tgagcgcaca caggctgctg
     7861 cgggcaaatg tcttgctcat aaagtcttac tcctgtcgta attaattgtt cagccgccgt
     7921 gccgtaaaaa tatctccttg tttaaccata gacggcggct agcaggcaca ccttcgcccc
     7981 tgagaaaatt taatcaggtt gtgagctacc gcgcattatg ccgtatgtaa ggttatcttc
     8041 tggagtatag acaaggagtt aggaaatatg actgctattc gaaattcata ttacccaacc
     8101 tgagaggaat ttaattaaac gctattacag ccgttcttga ataagtttgc ctaattgttt
     8161 aaccgcctgt tcttcacgtt ctccccactg ccacgcggta ttaaaacgga aaaaacgtga
     8221 ccagttttca ccggtagaaa acattttacc cggcgcaata ctgatatgat gcgtcagtgc
     8281 tgccaggctt aattcgcccg catctaacgg ctcggggagc tccagccaga gaaaataacc
     8341 gctgtcatta tgatgaattt tcacttccgc aggcagataa cgcagcagtg cttgccaggc
     8401 acgttgttta cgttccgcaa gctggcgacg caggcgacga agatgggcgt cgtatcggcg
     8461 cgtggaaagg taatccacca gcgcaagctg catcggtgag ctggtggaaa gcgtactcat
     8521 caactgcaag cgttgaattt tacgtgcatg ttttccggcg gcgacccaac caatacgaaa
     8581 accaggcacc agacatttcg aaaacgaaga gcaatgcaaa acaccatcgt ggcgatccca
     8641 cgctttcgca ggcagcggtt tttcccgtcc aaaataaagt tcgctgtaaa cgtcatcttc
     8701 aatcagcgtt acgttgtact gattgagcaa cgctaccagc cgcgcttttt tctgcggcgt
     8761 taaggtaaat ccgagtggat tctggctatt agtcatcagc cagcacgctt tcaccggata
     8821 ctcctgcaac gccagttcca gcgccttgag atcaatccct tctttaacgt ccgtcgccac
     8881 cgataacgcc ttcagccgta accgctccag cgcctgcaac gcaccgtaga aacaaggatt
     8941 ctctactatc acccaatcgc ccggttcagt gaccgcttgt aaactgaggt ttaatgcctc
     9001 taacgccccg gcagtaatga caatttcatc gggtgaaatg gtgatgccct gtaaggcata
     9061 gcgacgagct atagcctgac gcagttcagc gtttcctggc ggtaagtttt caatcacgct
     9121 catcgccgtg gcggttttgc ttacctgtgc cagcgagcgg ttgagttgtt ggagggggaa
     9181 aagtcgcggg tcgggaaagg ccgaggcaaa cggaactacc gacggatcgc gactggcctg
     9241 caacatatca aaaatatagg tgttgatatc gactgcttca tctcgagtga ctggaatgac
     9301 tggcgctttc ggcattttta ttgcctgtgt cgcaacgtaa taacccgact gcggtcgcgc
     9361 gataatatgt ccctgacttt cgagcaactg ataggcatgg ctgacagtca taaagctcat
     9421 gccagaaagc gccacctggt cacgcaacga aggcaaacga tcgccaggtt gccagatacc
     9481 cgacgcaatc tgctcgcgta attgttctgc aagctgctgg tattttttca ttatcgaatc
     9541 gtaattatgt gcagatgatt cggcagtcta tatcagttgt taaaaatgac aacttttcat
     9601 taactgttat caaaccatca ccagtgataa ctctttgcca agcgccttag ccgcaagctg
     9661 gacggcgtcg atttttgtag catggtgcaa gttaaataag cgagtaatct cctgtttagg
     9721 tttgccaatt cgtctggcta actcttgctg agtgatttct gactgtaaaa aagcatttaa
     9781 cagcaatact ttagaggcga tacttaaagg cacttcaata aagtgatcgt tgttagttaa
     9841 tggcgaaggc aacgggatta gctcattatc ttcaaaataa aaatcaaatg cggtgagtaa
     9901 ggcatctttt gctgcttcca tcgcttcagc aacagtttct ccctgcgtca acgcttcagg
     9961 gatatctaca aaagaaacca cataaccgcc ttccggcgca ggagtaagag tgacgggata
    10021 acgcataatt ttgactgtct ctcgcatgtc attaaccttc ttatggcgat taatttaaac
    10081 cgagttgctt caggattgct ttacgcaatg gttcttttat ctcatcactg gggtgacgcg
    10141 gcatgacact gcgtcgccct tgaaacctga gtttcaaatg gttgctgcca ttcgccacat
    10201 cgacgccctg agattcgagc caacgtctga actcgctttg tttcacatcg cctccctgcc
    10261 gctgagtata tcaacaaagt aaacattagt gtttacctcc gtgtgtaatt tgctcgaaaa
    10321 tagcactaaa tcgccgcaac ggcagaatgc aatgcaaaat caggaaggaa gattccagaa
    10381 tttctttgcg ctaatttgca aaactgaata ataacctcaa gaaacgatcg gcatcatagc
    10441 gacaaaaggt aagaaaaagg tggcaggatg tttacgaagg cgttatcggt tgtcttatta
    10501 acgtgtgcac tgttctcagg acaactcatg gcagggcaca aaggacatga atttatgtgg
    10561 gtaaagaatg tggatcatca gctgcgtcat gaagcggaca gcgatgaatt gcgtgctgtg
    10621 gcggaagagt cggcggaagg tttgcgcgag catttttact ggcaaaaatc gcgcaaacca
    10681 gaagcgggac atcgttgagt catttgttgc cggatgcggc atgacgcctt acccggcagg
    10741 caatatcatg cagatttgat gagttgtgag taaccagaaa actggcgttt tacccttttc
    10801 gcttcggtaa agttttcagc agctcatccg gacttacgga tgccactacg cttcccggta
    10861 acggcatttt cagtatgtga tttttgattt tgccaatgac gtgcatctcg catgggcggc
    10921 aatcaaactt tagcgttaat acttcatcgc cgttaaccag ttgcattggc gtcgccttcc
    10981 agcttttgat attgcctttc gcctgtttcg ggcacagatt gaaggcaaag cgcagacaat
    11041 gcttagtgat catcaccggg acttcgccct tctcttcatg cgcttcatac gccgcatcaa
    11101 tcagttgcac accatagcga tgataaaatt cacgcgcttt ctggttgtaa acgttcgcga
    11161 ggaaactcaa atgcgtttgc ggataaaccg gcgcaggatc agcaaccggt ttacggctgc
    11221 cgcgctggta actggcaaga cgcgcagcat ctagcatgtc ggcggcttca cggcggaact
    11281 ggtttaacag actgtttggt acaaacagcg cacccggcaa attaatttgc acatcgcggg
    11341 cgtaatagat ggtttgcccc agttttgcca gaccatcctt tagattgttc attgcttttt
    11401 cggcgttatt ggcttcgtcg aactgcccgt ccagcgtatg cgtgatgctg acaccctctt
    11461 cactggtgag ggtcagaatc agttgttcct gccagccgcc cagttcaatg tctaccgcca
    11521 cccgacgttc gctggaggtt tttgtcagtg cctgctgcca gttatgatca agattacggt
    11581 ttagtgggtg atgcgggcga attttgtgca actctgctgg catttcattg ggccagacgc
    11641 ggtactgatt ttctccggtt ttctcgaccg tattggcacg aaagccgacg acttcacgtt
    11701 taatcatcac gttcaggcca tcgccatttg ccagtggctc ggtaacggca acatcgagat
    11761 gatctttcgc cactttcaat acttcaccca ccggcaggcc gataaatttc ggcgaatcga
    11821 acgcgccaat atcgccttta cgggcattca caaaataatc tgtgctacca cggtggaaag
    11881 tcttttccgt cgatggaaca aagaaatgtt cagtacgacc tgatgaagca cgcgtcagat
    11941 cgccacgttc ttcaataatg gcatccagca tctggcgata atgggcggtg atattcttca
    12001 cgtagctcat atctttgtaa cgcccttcaa tcttgaagga gcgtacgcca gcatcaatca
    12061 gcgcgccgag gttggcagtc tgatcgttat ctttcatcga cagcagatgt ttttcatagg
    12121 aaaccacccg cccctgatcg tctttcaatg tgtatggcaa acggcacgcc tgcgagcaat
    12181 cgccacggtt ggcgctacgc cctgtttgcg catgagaaat gtagcactga cccgaatagg
    12241 ccacgcacag tgcgccatga ataaagaatt caatggtcgc gtccgtggcc tggtgaatcg
    12301 cgcggatctg ctcaagattc agctctcgcg ccagcacaat ctgcgtgaag ccaacatcag
    12361 agaggaactt cgctttttct actgtacgaa tgtcgcactg cgtactggcg tgcagttcaa
    12421 tcggcggaat atcaagttcc agaatcccca tatcctgaac aatcagcgcg tcgacgccgg
    12481 tctggtagag gtcggtaatc agccgttgcg caggttccag ctcatcatca tgcaaaatgg
    12541 tgttaagcgt gacgaaaatt tttgcaccat aacgatgggc aaatggcacc agttcggcaa
    12601 tatctttcag gctattactg gcattatgac gggcaccaaa accagggccg ccgatataaa
    12661 cagcatcggc accgtgcaaa atagcttcgc gggcaatggc ggcatcgcgt gccgggctta
    12721 acagttcaag tcgatgagaa gatacggtca cattcttatc cagtagctga aaaatggcgg
    12781 ctattttagc cacaagcgcg acgttgcgaa atagttttcc cgcttaactg cgtgggtaat
    12841 ggatgaggga atgaaaatgt acgatttgct ccccaccatt acgatagaca tgcgtaacgt
    12901 ctgcagcaaa tcgagcgcct tctccacaat taaggctctg ccattcacca tcaacgcaca
    12961 gatcaagttg cccatcaatg acgacaacat gttcgattac tcccttctca tggggcgtcg
    13021 attcacttat tgcaccaggt gccatctgaa tcgagaagtg ttcaaagcag agttgtggat
    13081 cataaggaaa cagcgaagtg atcaccatca cctgctgttg cggatcataa actgaaggcg
    13141 tagcggactg cggcggagaa ataaatgtcg aaaatggcac attcaacccg gtggcaattt
    13201 tccataatgt cgcgaccgtc gggctggact cattacgctc aatctgccct aacatcgctt
    13261 tagaaacgcc cgtcgcttct gccagtcgcg aaagactcca gccgcgctgc tgacgtagtt
    13321 gtttaagtgt ggtggataaa aagcgagtga gattttccat gcgactcctc cggcaaaacg
    13381 gaagtttatc acttgtgcgt tataacggac aaatgctacg gtacctgtat gctataacgc
    13441 acgaggtgac catgcgtctg ttttctattc ctccacccac gctactggcg gggtttctgg
    13501 cggtattaat tggctacgcc agttcagcgg caataatctg gcaagcagcg attgtcgccg
    13561 gagccaccac tgcacaaatc tctggctgga tgacggcgct ggggctggca atgggcgtca
    13621 gtacgctggc tctgacatta tggtatcgcg tacctgttct caccgcatgg tcaacgcctg
    13681 gcgcggcttt gttggtcacc ggattacagg gactaacact taacgaagcc atcggcgttt
    13741 ttattgtcac caacgcgtta atagtcctct gcggcataac gggactcttt gctcgtctga
    13801 tgcgcattat tccgcactcg cttgcggcgg caatgcttgc cgggatttta ttacgctttg
    13861 gtttacaggc gtttgccagt ctggatgatc aatttacgtt gtgtggcagt atgttgctgg
    13921 tatggctggc aaccaaggcc gttgcaccgc gctatgcggt aattgccgcg atgattattg
    13981 gggtcgtgat cgttatcgcg caaggtgacg ttgtcacaac tgatgttgtc tttaaacccg
    14041 ttctccccac ttatattagc cctgattttt cgtttgctca cagcctgagc gttgcactcc
    14101 ccctttttct ggtgacgatg gcatcgcaaa acgcaccggg tatcgcagca atgaaagcag
    14161 ctggatattc ggctcctgtt tcgccattaa ttgtgtttac tggattgctg gcactgtttt
    14221 tttccccttt cggcgtttat tccgtcggta ttgcggcaat caccgcggct atttgccaaa
    14281 gcccggaagc gcatccggat aaagatcaac gttggctggc cgctgccgtt gccggcattt
    14341 tttatttgat cgcaggtctg tttggtagtg ccattaccgg gatgatggct gccctgcccg
    14401 taagttggat ccagatgctg gcaggtcttg cgctgttgag taccattggc ggcagtttgt
    14461 atcaggcgct gcataatgag cgtgagcgcg acgcggcggt ggtggcattt ctggtaacgg
    14521 caagtggatt gacgctggtc gggattggtt ctgcgttctg gggattaatt gccggaggcg
    14581 tttgttacgt ggtgttgaat ttaatcgcta acagaaaccg ataataaaaa gcgccatcta
    14641 acgatggcgc aactggtatt actttttgtt aacgtcaaac atggtggcat cggtcgccat
    14701 gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt tattcaggtc
    14761 tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg catcaatcag
    14821 ctcaccttca aaatacaggc gagtatccat ggtacggtgg cccgtcgcca tttgcgtccc
    14881 cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt tgctggtatc
    14941 tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg taaccagtgg
    15001 tttacgctgc gcaatcgctt ctttcatttc ggtgttggta taactcaaaa ttttatccag
    15061 aactttctgc cctacctggg tagatggttt cggtaccgga taataagtga ctgggttcca
    15121 gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta aaacaggttt
    15181 acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac cagaatattt
    15241 atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc ataaagctgc
    15301 aactttcgca attgatgtgg tacgcatgaa tagtttcctg agatgattat attactttct
    15361 aagttatagc aaaaagcgcc gcttcgtgtt gtggcaaaaa aagtgttggt gaaaataaat
    15421 tttttagccc cttttcagac gacatagtca atgccgaaaa agacatagtc aatgccgaaa
    15481 aaggggctgc cgaaaaaggg gctaagtcaa tcatcagtag ttaaatttca gtaagttaag
    15541 ctgctaaaaa atttcgacta ctgtgattaa tttcacatcc attcgataaa aaatatgctt
    15601 accgaaaaga agaacagtaa accatctcag cgtggggaat tttcacgcgc cttgttccta
    15661 gcgggtcatt ttttacgtgc cagcatcgtg gcgaaacgca gtttaatacg attaccgttg
    15721 gcgtcggtgc ggtgcagctc gccgacgtct tcattgtatt tcaccatctc ccagccttcg
    15781 taatatcgac gtaattcccc ctctttgaag gcaaacggga agccgacggt acatggataa
    15841 tcagcggtat ccatcgccgc cacaatcagg ttgtaaccgc caggtttagt gcaacgttgc
    15901 atattggcaa tcagccctgg gatggtttta gcctcaagga acatcagcac cacagtcgaa
    15961 agaataaaat catactgtcc atcaaatgtg aggttattca gatcaacgac ccgggtgtgt
    16021 aaattctcca gattttcaat ggatttaatc cgctcgacgt tggcgatact catggcattt
    16081 ttatcccatg cgtcaacatc ataaccattg gctgccaggt aaagactgtt acgaccattg
    16141 ccacagccca gatccagcgt tttacccggt ttaaccactt tcaccgcttc cagcacttca
    16201 gagtgtgtgc gagttaattc atatttatca gtaaaatagt tttcgtcacg aatgatcatt
    16261 ctttgtcctc tgctttcatt aaaacggcgc gctcggttct gattaacaat tttccctgca
    16321 tcagaagcgc aaaagtacgg atgagcagta ttgcaataat aaaattggta aagataaaca
    16381 gcggcaccgc cagcgtatgg aaaaatccat tatcgctgcc actccccaga tgcaaaccgg
    16441 tggttgccag tgcagatacg ccgaacgaga aactccagaa tgaagcatta aatggctggg
    16501 agagatacca tggcatcaga cgtagcataa acagcagttg cagcagtcca taaccgaaaa
    16561 gcattttcgc cagcgtgtca ccctcgccgc cgttgacgct cagccaggca ctacaggcca
    16621 ccagcgcagg agcgagctga atgccgagtg atgtccgcag cgccgtgggt aattctcccg
    16681 aactgcgcag acgctgcaag atcacaggtt caaggcttag ccatgagaaa acgcctgcgc
    16741 ctaaaaacac cagaccggcg tcggtgtagc ccaacgcacc acaggccatt gcgctgataa
    16801 agttgttggc aactgtcggc agatacagtc caggcgtggt agcttcttca gggtgagatc
    16861 cgcgccataa tcccgcagtt tgccaggcgg cataagccaa ctgaaccacg acgccaaaac
    16921 tgaacaggca caccgccagt gggcgaaacc acggaacaaa accaatcgcc accagcatcg
    16981 tcgttgccgg aaacaaactc acaaaactgc tcagcactgg atggcgcact tccgccagca
    17041 cgctatgcgg aaagcgtatg agtcgggtaa taaatgcgct agttaataat ccccagatga
    17101 tcatcgccag aatcaccagc ccatccccta accagtggct gacctgccaa acctggctgg
    17161 catagcgcca ggcaaatccc atccctatcg tccccaacac aataccaaag tagcctgccg
    17221 gcaaattgag cactttatcg ctctgcatct tcttactcat tttgtttaat ttataaagta
    17281 tatttgaaat gcattttatg gaattttctc gagcatagcc agagccgcag aatttgctac
    17341 ggttatagta agtcacctgt gggaaaacgc tatgcgcaaa tatcgtctaa gtgaagaaca
    17401 gcgagccttt agttatcagg atgatggcac taaaaaaagt gtgttattac ggcagattat
    17461 cgccatgagc gattttaacg atgtgatagc aggaaccgct ggcggctgga tcgattgcga
    17521 aacggtgtta gcacaggaag gaaattgctg gatttacgac cagaatgcta ttgcgttcgg
    17581 cggagcggtg atttccggta atacgcgcat caccggcacc agcgtactgt ggggagaggt
    17641 ttacgcaacg gataacgtct ggatcgataa tagtgaaatc agtcagggtg catatataag
    17701 tgacagtgtc accattcgtg actcattagt ttgcgggcaa tgtagaatat ttggtcacgc
    17761 cttaattgat caacattcta tgatcgtcgc cgcacaaggc ttaacgcccg accatcaact
    17821 cctgctacaa atttatgatc gcgcaagggt tagcgcctcg cgtattgttc accaggcaca
    17881 aatttatggc gatgccgtcg tccggtatgc ttttattgaa catcgcgccg aagtttttga
    17941 ttttgccagc gttgaaggta atgaagagaa caacgtctgg ctatgcgatt gtgctaaagt
    18001 ctacggtcac gcgcaggtga aagctggcat agaagaagat gccattccga cgattcatta
    18061 cagttcgcag gtggcggaat atgccattgt ggaaggtaat tgtgtgttaa aacatcatgt
    18121 cttgattggc ggaaatgccg tggtacgtgg cggaccgatt ctgctcgacg agcacgttgt
    18181 aatacaaggt gagagccgta ttacaggtgc ggtgataatt gaaaaacatg tggagttaac
    18241 cgaccacgcc gtcgttgaag cctttgatgg cgacacggtt catgtccgtg gtccaaaagt
    18301 cattaacggc gaggagcgca ttacgcgaac gccgctggca gggttattgt gaatcgataa
    18361 tacgcgcgta taagttcaca tcatcatagg catcattcag gaactcagcc tgtttcagac
    18421 aaccttcaag gataaaacca ttgcgcaaag cgacctggtt gctttgcgca ttgtccaccc
    18481 gacatttgat cacgaagcgt ctaagttcac cagactgagc gtaatgatga atcaatgcct
    18541 gcagcgcctg agaaatgatc ccctgcccct gatgagattc gtccagccag tagccgattt
    18601 cagcggtttt attcagtggt tcaatacgat taaacgagat aacgccgata agttcatctt
    18661 ctttgaaaat catgaacatt ttggcatagc cgcgttgatg caacatcaca ttaccctgca
    18721 ccgtttttcg cgtgtcctct tcactttgaa caaactgcgg ccagtttagc gactgctgta
    18781 accaggttct atttttacag attaactgat aaagaggtgt gacatgactt tctgcaacag
    18841 catgtaattc aagtgattca cttactttta tcgtttcagt catcttatct ccagtcaatc
    18901 catgcagttg ttaaaaaagc cgcctggctg gcggcttagg aaagtttaca cctctttgac
    18961 gctcacctgc tgcaggattt tgagcatctc atcttttaac tgtagctttt gttttttcat
    19021 gcggaccact tccgcattgt accctcgacc gtcggaacct tcctttctgg caatttcatg
    19081 atcaagttta ttgtgtttat cgaacaagga cataaagcga ggattttcgt ttttcagacg
    19141 ggatattaaa tctcggtatt ctggaaacat acgcactccc tgttagtggc tgacaaggtg
    19201 tatctgaaat acacaccatc agattactca tttaacttag ggaaaaatgc gagcgggatc
    19261 gtgtttaacc agacggcacc tcccgaagaa gatgccgaag aaaaaacgat taactcatca
    19321 cgcggtcatc aacatactga cgtttatccg gcgctggcgg gaaatactga tacagccatg
    19381 tttcactgat agcgtcgccc tgacaacgca gatacatccg catatcgacc ggatcagtgg
    19441 agtccgaagt cggataccag tcaaactgaa tacgataacc atcaatgggt tcaatataga
    19501 gaatttcgat ttgtttcgct tccccactgg aaagcgtaat caccggctca atgccttttg
    19561 gcgcggcagc tttcagatca ccaccaacga aatcgacggc aaaacgtcgc gcccattttt
    19621 cgggatagtg ttcacctggc gcccaacctt ccgggaaacc gcccatgccg gtacgcgtcg
    19681 ccataacgcg cgctaatggg caatgaacag gcggttgcgc actccagtac agacgatact
    19741 ggaatgcaaa ctcatcaccc gcttttaccg ctttttctgg ctgccagaag cagacaatgt
    19801 tatccagcgt ttcgcccgtt gttgggattt ccatcaggcc gatggtgccc ttaccccact
    19861 tgttacgcgg ctccacccac agacttgggc gtttgttata ccagcccata atgtcctgat
    19921 aatgggagaa atcacgatcc agttgcaata aaccaaaccc tttcgggttg ttgtcggtgt
    19981 aagcattgaa ctgcaatttt tgcggattat tcagcggacg acaaatccac tcgccattgc
    20041 cccgccacat ggacaaacga tcagagtcat ggatttgcgg atgaatggtg tcgcacatcc
    20101 gacgttcatt agtaccgcag ctgaacatgc tggtcatcgg cgcaatgccc agctgtttaa
    20161 tgtctttgcg cgcatacagg tgattttcca catccataat cacctgactt ttctcacaat
    20221 ggatagtgaa cttataggca ccagtaatgc tggcgctatc gagcaacgca taaacggtaa
    20281 aggtggttgc ccccggtttt accgtatcaa accagaagtc agtaaagtcg gggaactctt
    20341 ctttactgtc ggtgtaagtg tcgatcgcca ggccgcgggc cgacaaaccg tattgatatg
    20401 tatcatcaac cgcgcggaaa taactcgcgc cgaggaatga tacgacatca cggcgcgcca
    20461 gttcgggggc tttaaacacg cgaaaaccgg caaaaccgag atcactttgc ccttctaatt
    20521 gtttggtatc aacacccgca tcgttgtatt tgaacaactc cgggcgaaag tgaatttcac
    20581 gcgccagatg tgttgctgga tctacagaaa acatacgaac gcggcgacgg aatcccattc
    20641 ccatatggaa gaactgagcg tccagttgac ggttctcaac gttatgccag agcgattttt
    20701 cggcgtcgta ttgaatactg ttataagcct gcggcgtcat tgtcgccaga gtgtcaggta
    20761 acggacgcgg cgcaccacgc cacgctgttt gcgctaagtc gtgcgccatt gactgtagaa
    20821 tggagaagtc aaaacgttgg gtttgcccgt cggcaatatc agaatctgcc gcgaatgccg
    20881 cctgagaaaa aagagaagca atgccgctgg taccgcacac ggcggccata gccattgaac
    20941 ctttaataaa tcgtctacga tccatacctg aaagtgagtc cttctggtgt tgtgaatggt
    21001 cccgcgctga tcgttttcag cggcaagaaa gtgcttattt atgaccgctc actctagaca
    21061 atatttatta ataatccaat tgttgaactg agagaatatg cataaatcgg caattttttt
    21121 atgtcgatgg gatgagcctg attcgtttgt aaaatcaggc aaatttttgc aaaggcggat
    21181 ggtcagaatt taaagcaaac cacagcgacg tttactgcgt tcttccgctt cctgataaag
    21241 ctggaactca tccaaaaccg ggcagcccaa cgcctgctca aatgcttcgc gactggcatt
    21301 accgtgactg cgtgcctgcg tttcattacc cagatttgac tggaagatcc ccgccgcgct
    21361 aacaggcaag aaatcttcat aggtaatggg ttgcgccact acccaaccac gttcaattaa
    21421 gggctgtgga tcgtctccgg gatgaatcgc ctgacgatgc gtctcacctg aaggcgtcag
    21481 acggtaccgg aaccatgcca acccttgctg acgcattaaa aactcactgt caggaaaagt
    21541 gcggaaggtt tcctgtaaat gcatttggtg agtgagatta tcctgcccgg ttccagcgtt
    21601 ccgcagtaga tcatcataca gttgtcgtcc tttcggcgtt aatgccacgc cacgctgttc
    21661 tatttcacca aagcgcgcgg tatgcgtgcc ctgtttctgc cctgcaaaca acaccgtctc
    21721 ttccagtgct ttaaagctag tctggcgtag taaaatcggt acctcgcggc gcggcggccc
    21781 ttcgatcaga attttgggtt caattccgca ttcaggcatc atcgactgca cccggtcaat
    21841 atccagcgtg cgtggcgtca ggtggttgat atggcatcca ggaaaacaga ccacatcagc
    21901 aatcaaccga tgttcgttgt gcaaggcgcg ataggtttct tcatctaccg ttgctgactg
    21961 gtgccaacga aacgtttcca gggcttcctg cacaaactcc tgtgcctgtg tttcgttaaa
    22021 accgccccgc tgctcatatt cctctaacag ttgtcgacaa cgtggggtga agatatcgcg
    22081 ctgacgcaga atctccgccg ctttctggcg caaaatttcg ttctcgataa gctcaaggcg
    22141 gagtaatgaa gtaaaaacgc ggaagggatt acgcgccaga gaagcatcat caatgggccg
    22201 aaatgctgtg gaatgtaccg gcacccccgc ctgcgagaga tcgtaatagc tcaccgggta
    22261 catccccata atggcaaaca tccgccgaag agtagcaagc tcttgtgcag tcccaacacg
    22321 aatcgcccca tgacgttcaa cattcagtcg cgccagctcg tctgcattta ccattttttc
    22381 gtgcagttga ggattgtttt ccagcacagc cagattcaca tcagctacca gttccagcag
    22441 cgtaccgtac tgcggaactt cttgctggta catggctgac attgcctgcg aaaactgttc
    22501 ccgaatctca tccgccgtga tgctgttcgc cataatgtct gcctccagtg gtcagtaatc
    22561 tggagtgtag gtaaccgcat tcactctggc gggaagaatt tgcaaactgt gatctcgccg
    22621 cgaaaacatc aatattatcc atttcgctgt aacataattg ctttaattgt taataatatt
    22681 ttgcaatcaa gttatcataa tcaaacaact tcacttgtca gcgacaccgc ttcgttttta
    22741 acatcgctta tggaaaaaaa tagtctgttt agtcagcgca tccgtttgcg ccaccttcat
    22801 acattcgtag ctgtcgcaca acaaggaact ttggggcgcg cggctgaaac ccttaatttg
    22861 agtcaacctg cgctctctaa gacattgaat gaactggagc agctgacggg cgctcgcttg
    22921 tttgagcgtg gtcgtcaggg ggcgcaactt accttacccg gcgaacaatt tttaacgcat
    22981 gcagtcagag tgcttgacgc catcaacact gctggacagt cgcttcatcg taaagaaggt
    23041 cttaataatg atgtcgtcag ggttggtgca ctaccgactg cggcactggg gatattacct
    23101 tcggttatag gtcagtttca tcagcaacaa aaagagacga ccttgcaagt tgcgacaatg
    23161 agtaacccta tgattcttgc gggtttaaaa accggggaaa tcgatatcgg cattggtcgg
    23221 atgtcagatc ctgaactgat gaccggactt aattacgaac tgctgtttct tgaatcgctg
    23281 aagctggttg tccgccctaa tcatccgcta cttcaggaga acgtaacgct aagccgggtg
    23341 ctggaatggc cggtcgttgt atcaccagaa ggcactgcgc cacgccagca ttcagatgca
    23401 ttagtgcaga gccagggatg taaaattcct tcgggttgta tcgaaacgct gtctgcttcg
    23461 ctatctcgtc aacttacggt tgaatacgat tacgtgtggt ttgtcccttc tggcgctgta
    23521 aaagacgacc tgcgtcatgc cacgctggtg gccctgcctg ttccgggtca tggtgcaggc
    23581 gaaccgattg gaatactgac ccgcgtagat gcgacgttct cttctggttg ccagttgatg
    23641 attaacgcta ttcgaaaatc aatgccgttc tgaaaggtga agggatctgt cgatccctcc
    23701 tttaacattt tcacactgta gcgaaactaa ctggttcacc agctccgcga ggttctgccg
    23761 acacagaatg tttgttcaga cggaatacgt ccaccgcctc agttaatcgt gccgcctgtt
    23821 cttcaaggga cactgccgct gctgaggcct cttctaccag agaagcattc tgttgcgtca
    23881 ccttatccat ttcagatatc gcctggctaa cctgcgttat acccctactt tgctcatccg
    23941 aggcggcggc aatttcctgc atgatatgtg tgacactcgc gacggcatca acaatagtgc
    24001 tcatggtttt cccggccgtg gccacctcat ccgatcccag gtcaattaac ctgactgatt
    24061 cactgatcaa gccttcaatc tctttcgccg cctgagcgct gcggcttgcg agtgtccgta
    24121 cttcgctggc gacaacggca aatccacgcc cttgctcacc cgctcgcgcg gcttcaacgg
    24181 cagcattcag tgccagaata ttcgtctgga aagcaatact gttgatgacg gcggtgatct
    24241 cagaaatttt cttcgaactc gtggagatag cgcccatcgt ttttactaca ccggaaaccg
    24301 tctgcccgcc atcgctggct ttaatagaag cctcttgcgc cagtttgctg gcatgatgcg
    24361 cgttatccgc attctgtttc accgtcgcag tgagttgctc catgctggcg gcagtttgtt
    24421 cgattgccgc cgcttgttct tcggtgcgag atgaaaggtc cgcattgcca gcggaaattt
    24481 cgctggtgcc acgataaatc tcttccgcac cctgtcgaac agtccctact gtcatcccca
    24541 gtgaatgctg catttgctgt aaatgacgac ttaagcgacc gatttcatta cgacccgccg
    24601 gttcatcatt catcgtcaga tcgccactgg cgattttttc aatccgttgt gcggcatgtt
    24661 gcagtggacg aatgacgatc cgacgtagca ccataaatgt tatcagcgtc atgaccaggg
    24721 caagcacaaa cgcgccaatc atgaacatcc cacccaggcg ggtgcgctga tgggccagtt
    24781 ccgccagttg gttggctctg gtgctacgta tcttaacggc tttgctcaaa atatcggtat
    24841 aagcattatc cagcggtcgg atctgctcac tttcatgatt gataatcgct tcaaacatgc
    24901 catttttggc atatttcaac ataggttgca tacccgtgat ataagcctgg aagcgttgat
    24961 ttaattcagt gtcgagggct tcatcagcag gtgttttcac cgaccgattc tgataagcac
    25021 gataaccttg ctgcgactgt ttaatctccg attcggcttg cgcaatattt cgcttcattg
    25081 cttccatttc cgcaatacga ctcgctgccc cggcctgaat catattgatc cgggctgaac
    25141 gcaaaaaatc tgaactgttc gctaacccgg tccgaatctc aatctccgca gtaacatcac
    25201 gctgatcgcg atcggcctgc cagaggaaat agccagccag agcagaactt aatgcgaata
    25261 gaactaagat accgccaaga atgcaggcaa ataacggaac cagcctgatg tgatgcaaaa
    25321 aacctaatcg ctgtgaggga gttgtattca ttaccgttgt ctctcgtcca ggtttactcc
    25381 tgatagaaag tcatcggcac ttgacggtaa ttacttatga ccaaaaacct ctttgcgtca
    25441 catttttaca acaattatgg cagagaagaa taatgccatt ggaatgaaga gagtaacgaa
    25501 tgattaaact ggctaacccg aatgacgaaa aattctggaa aacagcacgc aatttcgtca
    25561 ggtgattatt ttcgtcccta aggaaaccac ataggatgct cacgggtgcc tgagactttc
    25621 tgttttcagg ctaaccggga atcagaaaga cgaaggcccc gaccgatatt tctatcaacc
    25681 gaggccaacg aatgaacaca gcaattcaga tcttagcctc ttacccgcca tccggcaagg
    25741 agaaaggcta tgaagcgcaa ccctctggtg gtgtgtctgc tcattatctg cattacgatt
    25801 ctgacattca cactcctgac ccgacaaacg ctctacgaac tgcggttccg ggacggtgat
    25861 aaggaggttg ctgcgctcat ggcctgcacg tccaggtaag ggcaagcgcg gggattttcc
    25921 ccgcgcatct gactagcaga ttcattttct catggcaccc cttttacact tcagttaatg
    25981 catgtttttt tcgatgcact aatagaacca tcattacaaa caaatttgcc gcctgccgta
    26041 cagtgtgaga tccccccttt cgaaccagaa cagggtttgc gcccggcatc ggcataaaag
    26101 gaaacaagcg ttcctataac caagataagt gccagttttt tcatataacc tccatggttt
    26161 atcgaatctt atgttcgatt ttaacgttat acgctggttt attttgatac cgttgatgag
    26221 atcaaagatc gggtgaattg attaaaaata agccagatgg acgtatgggc aaggattatg
    26281 gttttatttg tcatacaaat aaatataata ggcgcttccg atgtagaccc gtattcttcg
    26341 cctgtaccac gggtcggttt tagtacaggc gttttcttta aatatccttc aggaacgttt
    26401 acgcggcatc atgcgtagaa gtgtgttgtc cttccagaaa tagtggtgtg ccagtgcagc
    26461 cgcagcgtgc aacccgatga caaaatatcc cagattcgcc agcatttcat gccacgactt
    26521 taagctatct acccgttcga aattggcctc tgaagcgtaa ggcatcgtca aaccaaacgc
    26581 aaaccacggg ttgccccggt tatacatcat caccaaacca atcactggca gcgcaataaa
    26641 caggagataa atcaccaaat ggcccaaatg cgccagtccc gtcatcatcg gttttggctt
    26701 aggtataatc ggcggggttg ggtatttcag tcttaacaaa agacgaacca ccatcagtac
    26761 gagaattgag atgccacagg aaacatgaat catgttgatg aggggccgat cgctacgtgg
    26821 gaagaaacca cgaaactcca ttgcgcaata cgctacgata accagcaaaa agaccagcca
    26881 gtgaatgctg atttgtaacc ttgagtattt atttcccata acatttcctg ctttaacata
    26941 atttgccgtt aacataacgg gcttttctta aaatttcatt aaatattgtt cacccgtttt
    27001 cagtacgtta cgtttcactg caagaatgta gattgcccat cccgccatcc tggtctaagc
    27061 ctggaaagga tcaattttca tccgaacgtt cctgacagga gaagaccatg agtaaagttg
    27121 gtattaatgg ttttggtcgt atcggtcgac tggtgttgcg tcgattactt gaagtcaaaa
    27181 gcaacataga cgttgtcgct attaatgatc tcacttcccc aaaaattctc gcctacctgc
    27241 tgaaacatga ttcaaactac ggaccgttcc cctggagcgt tgattttacg gaagattcac
    27301 ttatcgttga tggaaaaagc atcgcggttt acgccgaaaa agaggctaaa aatattccgt
    27361 ggaaagcgaa aggggcagaa attattgtcg aatgtactgg cttttatacc tccgccgaga
    27421 aatcgcaggc gcatcttgat gctggtgcga agaaggtgtt gatttccgcc cctgccggtg
    27481 aaatgaaaac tatcgtttat aacgtcaatg acgacactct ggatggcaac gacaccattg
    27541 tttccgtggc gtcatgcacc actaactgcc ttgcgccgat ggccaaagcc ttgcatgaca
    27601 gtttcgggat agaagtcggc acgatgacga ccattcatgc ctataccggc acccagtcac
    27661 tggtggatgg tccgcgtggg aaggatttac gagcttcacg cgcagcggca gaaaatatca
    27721 ttccccacac tacaggtgcg gcaaaagcca ttggtctggt gatcccggaa ctgagcggca
    27781 aactgaaagg tcatgcgcaa cgcgtgccgg tgaaaacagg ttcggtcact gagctggtgt
    27841 ccattctcgc aaaaaaagtg actgccgaag aggtgaataa cgcacttaaa caagcaacca
    27901 ccaataacga gtcatttggt tataccgatg aagaaatagt ctcttccgat atcattggca
    27961 gccatttcgg gtcggtgttt gatgccacgc aaacggaaat taccgccgtg ggcgatttac
    28021 aactggtgaa aacggtcgcc tggtacgata acgaatatgg cttcgtcaca caactcattc
    28081 gcaccctcga aaaattcgct aaactctgac gcacacaggc ggaggaaaaa cctccgcctc
    28141 ttccactcat taagactgta aataaaccac ctgggtctgc agatattcat gcaagccatg
    28201 tttgccatct gcgccgccaa taccagattt acgccatccg gcgtggaatc cttgcatagc
    28261 ttcgaagttt tcacggttga tgtaagtttc accaaacttc agccctttaa tggctttcat
    28321 cgcgacgttc agattttggg tatagattga tgaggtcagg ccgtaatcac tatcattcgc
    28381 cattgagata gcctcttcca gcgtgtcaaa tgcgaccacc ggcagaaccg ggccaaaggt
    28441 ttcctcatgc ataatcgaca tttcctggcg aacatccagc agcaatgtcg gcggataata
    28501 atatcctttc ccctctaccg ctttgccacc caacgccact ctcgcccctt cttctactgc
    28561 gcgcgccact ttttgctcga ccctttccag cgccgcggcg ttaatcaacg gccccatcgc
    28621 aatgtcgttg cgttcagcgg ggttaccaaa ttgaaccgcc tgcatcgctt cacccagccg
    28681 attgacaaac tgatcataaa tgcctttctg tacataaaca cgttctgcac agttacacac
    28741 ttgcccacta ttaatgacgc gtgaatcaac gatggctttg actgccagtt caagatcggc
    28801 atcgtccatt acgatagccg gtgctttacc ccccaattcc aggcacactt tggtgatgtt
    28861 tttcgccgca gtcgccataa tcttttcacc tgcagagacg ctgcctgtca tactgaccat
    28921 tgcgaccttt gggttacccg ccagttcttg cccaacggtt tcaccacgcc ccagaacaag
    28981 gttaaacacg ccgcgcggaa ggcctatttc atcgacaatt ttggcgaatg caatcgcatt
    29041 gtttggcgta aattcactgg gtttaatgac gatggtatta ccggtcaaaa gagcgggagc
    29101 cattttgcgg gcaatgagga agaacgggaa gttccacggc agaatgccgg tagtcacgcc
    29161 aagcgcacgt ttaaacagaa gaatattttc tcctggacga tcgctttgaa taatctcgcc
    29221 ttcataacgc cgtgcccact ccgccatgta atcgatatag tccgcagtaa aagccacttc
    29281 gacttcagcc agctgctgga tcttaccccc ttcttcaaca atcagcgtac tgatttcact
    29341 ggcgcgttcg cggatcccgg cggagatttt gcgcaaccaa ctggcgcgtt caatagcagg
    29401 caacgcttcc cattctggtt gtgcacgttc tgctgcatcg attgccttac gggcatcctc
    29461 ggcctgacca tcgggaatgc gggaaatgac agcctctgta gcagggttta ccacatcaat
    29521 ccatgcgtct ccacgccagg taacaaactg tccatcaata tacataggat gttgaacggg
    29581 tactgacatg agcgactcct gtgatttata tgtttgtttt tggtaaatgt attgttaatt
    29641 aacatcaatc atcatagaga cggcatcatg aaaggacaat tctgtgaagg gcttcataaa
    29701 aacaagtata aaaggaataa gaggcagaaa taaaaggcag gagttatgcc cgtttgcaaa
    29761 caagtggttc acaaacgggc aatttttaac gtaacgagcg actttccatc gcctcgatta
    29821 gcaccgcatc gtgtttcagc gtttgccatg catggatgac ttctgccgga aaatcaacgt
    29881 gaacgataaa atctcgcccg cggggaccgt agccatcgct atcatcgcgt aaacgcggca
    29941 gctcgccagt gagtagtgag agataacgct caaccggcca taatccttgc aacggattaa
    30001 caaagattac actgtctgcg ttatttccta actgaggaac gaatccggga taacttaacc
    30061 agcgtggtgc atcgggattg tccccagtca tacggcggaa cgtcgccatc gtgcgccgct
    30121 gcatggtggg gtcctgaaca acgatagccg tatgaactcg ttctacggcc tgattcaata
    30181 gcgcgatgct aaagcgtgcg ttttcaccgc agtttgttga ctggtcttca atccagattt
    30241 tttcatgcgg aatgtgccag aactgatgag cgatatccgc caggatggtc gcttctgctc
    30301 tgccagtggt gcggatagtg ttgtagtgcg gatgctgtgc gatggcgcta tacaaaaaag
    30361 ttgtcgagtg accgatacca ccactaatca gtagaggaat ttgctgatcg cgagcaatct
    30421 tacatgccgc gtcgatagtc ggcataaccg cattgcctgc aaggatcacg caatcggcct
    30481 gatacggcat ctcaccggag aaatcatcct gcgccagcca ctgaccgata acatttatgg
    30541 catctatggt tgccggcgaa agcgtcggaa acggggtaat gttcatggtt cctccttatc
    30601 gttcccttaa gaatactgcg tgagataata gtgagaagcg gagcactctg aaaggaggaa
    30661 gtcgtatttc cttaaccgcc actccgcaag cctgacatat cttttaagga aatgctgtag
    30721 ataaacatta ttcatcttgc agctgagaat tgtatttttc tgaattcctg aatttttgcc
    30781 cttaccgttt tagaattcat aaaaaacccg gcgctaaatg cttaccgggt tttagattta
    30841 tcaggcaaat agccgcgtta accgctaatc tgttccatcg cctgcaaaat acgcttatct
    30901 gaaatcggat aaggcgtacc aagttgttga gcgaagtaac taacgcgcaa ctcttctatc
    30961 atccaacgga tctctttcac gtcttcatcc tcacgacgtg cgggcggcag tttgttgatc
    31021 cattgctgcc acgcctgctg gacattttcg actttcagca tctgagcacg gtcacgatgc
    31081 ggatcaaccg ccagtttttc cagtcgtttt tcaatcgcct gcaaataacg cagcgtgtcg
    31141 cccagccgtt taaaaccgtt accagtgaca aaaccgcgat acaccagccc gcccatctgc
    31201 gctttgatgt cagaaagccc cagcgccata gtcatatcca cccgcccttt caggcgtttg
    31261 ttgatattga acactgccgt aaggatttgc tcgacctgct tcgcaatatc caccaccgta
    31321 tcgttcagtt cagcgcggac tttctcatgc agcgcagcaa agccttcttc cgtccagacc
    31381 gggcctccat tggcgtcgat cagttgatcc accccgcagg agatacagtc gtcgatcagc
    31441 tccagcactt tgccatacgg gttaaagtac agccccagct tggctttgtt cggtaacttt
    31501 tcatgcaaat acttgatcgg cgatggaata ttcagcagca gtaaacgacg aagaccgttc
    31561 cacatcgctt gcttttgttc cagcgggtta tcaaacagtt tgatcgccac gctgtcgcgc
    31621 tcatccacca acgctggcca cgccttcact ttgtagttgc cacgcttctg ttcgtagctt
    31681 tccggcagtt gcccaaagct ccagatatgt aagccgctct gctcgatacc gtcatccgcc
    31741 accgcagaca gcgtttcctg cactttgcct ttcagagcat ctttcagatc ctgtaatgag
    31801 cgcccttctt ttagcttctt gtttttgtca tccaccacac ggaaggtgat tttcaggtga
    31861 tcgggcacct gatcccagtg ccagtcttcg cggtcaacgg tgacgccagt catccgccgt
    31921 agctctcgtt caagactgtc gagcaacgac aattccaacg gtgtgacgcg gcctagaaac
    31981 gcttcggcgt agtttggcgc gggcacaaaa ttacggcgta ctggtttcgg caacgattta
    32041 atcagagcaa tcaccagttc gcggcgcaga ccggggattt gccattcaaa cccgctctct
    32101 tctacctgat taagcagcgg cagcggaata tgcacggtca caccgtccgc gtccgcgcct
    32161 ggctcaaact gatagctcaa acgcagcttg agattgccct gatgccagaa gttcggataa
    32221 tccagcttgc tgattttttc cgccccttct ttgatcaaca tgctcttttc aaagttgagc
    32281 aaatcaggcg tttcgcggct gacttttttc caccagctat cgaagtgacg agcggagatt
    32341 acatcgtggc tgatgcgctg gtcgtagaac tcaaacagcg tttcgtcatc aaccagaata
    32401 tcgcggcgac gtgatttgtg ttccagctct tccacttcgg cccgcagttt caggttttca
    32461 cggaagaatg cgtgacgcgt ctgccagtca ccttccacca gagcgtggcg aataaagagt
    32521 tcacgacata acgccggatc gatctggctg tagttgacct tacgcgcagc aacaatcggc
    32581 aaaccataaa cagtgacttt ttccgttgcc atcaccgcgc cctgcgcccg ttcccagtgc
    32641 ggttcgctgt aggtgcgttt aatcaaatgc tgagcaacgg gttctaccca ttcagggtcg
    32701 atacgcgcag caatgcgccc ccacaggcgg ctggtttcta ccagttccgc caccattacc
    32761 catttcggcg gctttttgaa taaaccagaa ccgggaaaga tggaaaaacg cgcgttacgt
    32821 gcgccggtat attcttgttt atcggcatct ttcatgccga tatgggaaag caaacccgtc
    32881 agcaacgcaa tgtgaatttc gcgatactcc gccggttcgc tgttaaccgg aatgccaagt
    32941 tctttcacca cctggcgcaa ctgggtgtag atatcctgcc attcgcgcac gcgcagatag
    33001 ttgagataat cggtacgaca caggcgacgg aaggcgttag aagaaagcgc cttttgctgc
    33061 tcgccgagat aattccacag atttacaaac gcgaggaagt cggattcttt gtcgtggaag
    33121 cggcgatgtt tttcgtctga tgcctgctgt ttatccatcg gacgctcgcg cggatcctga
    33181 atggagagcg cggacgtgat aatcatcgcc tcacgcacgc agccatgttt ttgcgcttcc
    33241 agcaccatac gcgccagacg aggatcgaca ggcaattgcg aaagctggcg accgagcggc
    33301 gtcagtttat aggcgctggc ctgttcatca gtggtgatcg cgcccagctc ttcgagcaga
    33361 cgcacgccat cctggatatt acgtttatcc ggtgcttcga caaatgggaa cgcggcgata
    33421 tcgcccaacc ccagcgcggt catctgcaaa ataaccgagg ccaggttagt acgcagaatc
    33481 tccggatcgg taaactccgg gcgcgagagg aaatcgtctt cggaatagag acgaatacag
    33541 atcccttcgg acacacgacc acagcggcct ttacgctgat tggcagacgc ctgagaaatc
    33601 ggctcaatcg gcaaacgctg cactttagtg cgatagctgt agcggctgat acgcgccgta
    33661 cctgggtcga taacgtattt aatacccggt acggtcagcg acgtttccgc gacgttggtc
    33721 gccagcacaa tgcgccgtcc actgtgcgac tggaacacgc ggttctgttc gctgttcgaa
    33781 agccgcgcat aaagcggcaa gatttcggta tggcgtaagt tcagcttgtt cagcgcatcg
    33841 gcggtatcgc gaatttcccg ctcaccactc ataaagatca gaatgtcgcc agggctttcc
    33901 tggctcagtt cgtctacggc gtcaaaaatc gcctgcagct gatcgcgctc ggtgtcatcg
    33961 gcttcttcaa caatcggtcg atagcgcact tccaccggat aggtccgacc ggagacttcg
    34021 ataatcggcg cattattaaa gtggcgcgaa aagcgttccg ggtcgatagt cgcggaagtg
    34081 ataatgattt ttaggtcagg acgccgcggt agcaactctt tcaaatagcc gagcagaaaa
    34141 tcgatattca ggctacgttc gtgcgcttcg tcaataatga tagtgtcgta ctgcatcagc
    34201 aggcggtctt gctggatctc cgccagcagg ataccgtcgg tcatcagctt gaccatcgtg
    34261 ttatcactaa cgtgatcact gaaacgcact ttgtaaccga tgcaaccgcc cggctccgtt
    34321 ttcagctctt ccgcaatacg gttcgccacc gttcgtgccg ccaggcgacg cggttgggta
    34381 tgaccgatca gccctttaat cccgcgcccc agttccatac agattttcgg tagctgagtc
    34441 gttttaccgg aaccggtttc cccggcgacg atcaccacct ggtgatcacg aatcgcttcg
    34501 agtatgtcct gctttttctg actaaccggc aaattgtccg gataagtgat ttccggtcgt
    34561 gccgcttcac gcagcaggac tttccctgcc gcatggtcaa tctctttcgc catctcctgg
    34621 aaaatggcct gttgtgcatc aggattttta accttcttca cgccgtgcag acggcgagaa
    34681 aaacgcagtc tgtcgcgcag catcagcgaa tccagccgct gctgcaaggc cgtaaaggtc
    34741 aatttttgtt gttctgtcat aacgttagtg ggcagtagtc tgcctgatta attttctttt
    34801 aaatgataga tatagagtac cacatcgcgg ctttctatgc gttgttcaat aaattcgaac
    34861 ataggcttcg atttattgcg ctatctctgt ggcaggattg tgaataaagt gtcaacaagc
    34921 aacggggcat cgcccattca aacatctata aggaaacacc atgagcaagg tattagttct
    34981 taaatccagc atcctggcag ggtactctca gtctaatcag ttgtccgatt attttgttga
    35041 acaatggcgc gaaaagcact ccgctgatga aatcaccgtt cgcgacctgg ctgcaaatcc
    35101 gattccggtg ctggatggcg aactggttgg tgcgctgcgt ccgagcgatg caccgctgac
    35161 tccgcgtcag caggaagctc tggcactttc cgatgagctg attgccgagt tgaaagccca
    35221 cgacgttatc gttattgcgg caccgatgta taacttcaac atctcgactc agttgaaaaa
    35281 ctattttgac ctggttgcac gcgcaggcgt tactttccgc tatacggaga acggtccgga
    35341 aggcctggtc accggtaaaa aagccatcgt tatcaccagc cgcggtggga tccataaaga
    35401 tggcccaacg gatctggtga cgccgtatct gtccacattc ctgggcttta tcggcattac
    35461 cgatgtgaaa tttgtcttcg ccgaagggat cgcatacggt cctgaaatgg cagcgaaagc
    35521 gcagtctgac gcgaaagcag ccatcgacag tattgttgct gaataactgg acacgatctt
    35581 gatgtggact gtagatattc agtccacatc tcaatccgct tacctgtttt cccataattt
    35641 cagcatcgct ttgtgctctt ccttaagcac aatccgcgag cgtctggctc gaatataact
    35701 aatcgcttca tcaacggttt tacaatgtcc gtaacataac agccatgccg ccaccaccag
    35761 cgcactgcgc gataatccca acgcgcaatg gaccagaacg ctgccttgct cttcgcgtaa
    35821 tgtttccagc atcgccacgg cctgtcggag ctccccctct tccggaacca ccagatccag
    35881 catcggtaca caaaaataga gttgatcttt tgtggctcgt ccgcgaggga attcaaaggt
    35941 gacgtccaga acagcattct gtgccggaat atgttgtgga aacgccccta aataaacacc
    36001 agcagtaatt ttgctcaccg actccaggcg aagacaaaac caacgcatag acagccacat
    36061 cccgatgcgc cagggcaatg tcagccagta aacggcgggc gggagtttcc cctaactatc
    36121 tttccctgtt gttatcgcgc caagcccacc gtacccacgg ccaatgatga atagcgataa
    36181 tactggccaa cataaccaga ctgaccacca taactgaagc attatcatta gctccatcaa
    36241 cacaatgcac gagcacgcgc ctacgacata tggcagtgct attttgattc gacgttgatc
    36301 aggtttctga taattccaac gacggtcgac gggcaccatc cagtcaatta acatacctac
    36361 cgccagccct gtgatgacat caataaaatg atgctgccag gtcgtcagcg tcgaaatggc
    36421 gatgagtaaa aaccatccgc cgcagacttt acgccacctc acagccagat gctgacgaaa
    36481 gtgacgccaa agtagccagc agagaataat atgcagcgaa ggagactggt tataaggcag
    36541 atcaaacaat tcaagttgcg aaaataacca tcctgtcacc ccactcactt caggacggat
    36601 aaaactaaac ttcagcggat agagaaaaaa accgcagcag gccattaccg ttgccagaat
    36661 aagccggtgg accagacggc gctgctcaaa tgtcgagcta caaacaaaca gcgaaaatcc
    36721 atataaaaga tccagactcc agtaaggaac aatagtccag ggaagaaaag ggatcgccgt
    36781 ttcccaaccg aataactgac tggggatatc atggttgtta agatcctgag tcgcggtgaa
    36841 ctgattaaga aatccatatg tgaagaagaa aaacggggcc agcaacaata accagccagc
    36901 gccttgtagc aatacgttcc ggcgttcagt taccacgacg caccgccatc gaaaccgtaa
    36961 aaatgcccca ctcatcaatc cgttgtgtgc atttatcgaa tccggcatca tgcacgagcg
    37021 aatccatctc cccttgagaa cgcacgcgca ttacccacgg tttaccatct ttatgactgg
    37081 ttaacacccc ggcaatcagc tccagttgtg ggtgccacgg ctgcccggtg tagatgagaa
    37141 tgccacccgg atcgatggca ttggcaagac ctgcgagtga gttttttacc tgctcatttt
    37201 cgggaaaaag ctcatacagt ccagagacaa tcgccagcgt aggccgcgga gttaacgcgc
    37261 tgagctcctc cgggttaaac gcatcgccct gttcaaaacg cacccgccca gacattcccc
    37321 gttgggcaat catctcttgc ccctgtgcaa catttaactc actgtaatca cgtaagaaaa
    37381 tatcgcttac ggcaggctcg ttcgccagcg catccagtac atagcgtcca tgccctgcgg
    37441 caatgtcaac cacgcggacg gctaaacctt tagcgtggag atcggcaacg gcttgtttaa
    37501 tcagcatttg taaatgggtt ttgcgctggc gaataccgcg ccagccaaca ctgttcaggt
    37561 agattttgtc gactaaacgc ccgaatgcgt tactaccttg cggttgattt tgatagacat
    37621 aatccagcga actgcctgaa tcaaagccgg tgctcattcc gagatgcagg cccgcagagt
    37681 gcgccccgaa caatttcatc gctttacgca taaagcgata cgccaaatca accggcgata
    37741 atggcacggg tccaccagaa agtagccgcc atcgatccgc tgatggtcct gtgcagtctt
    37801 catgctgata atctaatttt tgcgatttgt tagcatataa gtgactaata aagctttgca
    37861 ttttttcaaa taccagcgcc ctgttctctt cacccaacgt gtcgtgataa aagcctggca
    37921 gcagatgcag ctctttcaga gggctacgta aacgctgata aaaatcaatc tgcggttggc
    37981 ggtgcaccac atagtcatcg cctgatatca gaagttgcgt ggggagcgta atcgccgccg
    38041 catcgctaat aatacgttca gacgttttat agagatctag caagatattg acggcaatcg
    38101 cccttgtgat cagcggatca ttattgaaac tagccacccg ttgccgatcg tgggtcaaat
    38161 agcgtccttt cacataggaa ttaataaaaa acagaccacg cagacgatgc cataacgcca
    38221 gcgcaggacg tgccagcggc acatacaatt taaccttaaa ggccggagaa gccagcacca
    38281 gcccgcgaat agcaggcgca taatcatgaa cccatgtagc aaccatcact gcgccgacgc
    38341 tttgcgcgat cacaaccacc tcttccagtc cgacctggct gtcgctggca gcaaaacgga
    38401 caaattcatc gacatcttgc actgaactcg caagagacgg actataacca cgcggccccg
    38461 aagtttgtcc atgccctcgg gcatcccatg cataaaaagc agtatctggc atcgccagtt
    38521 catcaacgag atgttgtaga cgaccagaat gttcatgacc gcgatgaaat aagacgatga
    38581 cctttttcgc ccctggctgt aaagtgggcc agtgccgata aaacaacgct gtattatcac
    38641 tggtggtaaa aaaatgttcc ccagggatgc gtgaattttc cattctctct attttccttc
    38701 agtaacagca atagcgaata aaataaaaaa ataccggagc ggtgaaaatt aatgagtcaa
    38761 tcctgtcaag gagtccgcca tgtcctggta atagttttcc actatcttta acaccaacat
    38821 ctcgtttgat ggctgacatc actacatcgc cgcaaaaacc actaatacca attaacaaac
    38881 ccgctaataa tgcctgtaat gtatctagcg gtgtcagtaa cggtccaata atcagtgaca
    38941 caatcatggt agtgattacg ccccccacca gaccttccag cgtttttccc gggctgactt
    39001 tggggaccac ttttcttctg ccgcaggatt tcccccataa atactgtgca atatcgtttg
    39061 actcggttaa tgccaaaaga aataacacca gtaacgcctc gccctggata tttgtggttg
    39121 gcaacattaa taaccaggcg gcatgactca aggcgaaaac ggtcattatc cagccacaaa
    39181 aaatggcgct cacggtatgc agaaaaccgg agggttcacc cacaaaaact cgccaggtgg
    39241 ctaatatcag aaacccaaac aaaggtataa ataacagaaa cagctcaaag caatttaaac
    39301 cgataagcaa atagttaaga ggaataaccc aatataacca acgcggaaaa tgtacggata
    39361 taagcgtaca atattctttc aatgccagaa aacttatcag cgcaaaaaac gtcaacgtca
    39421 cccagcgtgg acctgaaatc accagtgaaa aacaaatgac gatgccccac caggtacgaa
    39481 tacgtaatgt aacttcatcc ccttttctct ccggtaaccg ccataccaga aaacggttta
    39541 tcagcgttgc taaaattaac aacgcgaaaa gtgttgccag agatttttcc agcattattt
    39601 ttcagcgttc atcaggagca cactccgaca acgattaatc gcagtccaga gaagcagaat
    39661 tgaagcaatg ctccataaaa gattattcca ttgcatccac tgtggataaa tggcaacggc
    39721 cagcacccac aacccaaata ttaacgcgcg atcgcttttg ccaaatggac cggcataact
    39781 gcgaataccg ttaatcgtct gcgcgagtaa accgcaaaac tcagtcaata tggtgcaaaa
    39841 taacatgagt attacgagtg atgcgttact ttccggtaaa aataaaaacg gtaaataaag
    39901 cgcaatatcg gaaataacat cgccagtttc attcaaaatc gccccaagac gtgtttgctg
    39961 attgcactca cgcgccatca tgccatccag cgcattgagt gccatacgga caaaaagcac
    40021 gatgggcaat agcagaaaca tgccatgata tggcaccaac gccaacaaca aaccggtgag
    40081 aagagaaagt gccaacgcgg caagggtgat ctgatttgca gacacttgcc gcttatgaag
    40141 ccaaaacatc gtcggcctta acagtgactg aaagagcggt tttatctggt agagcgtcat
    40201 tatcaatcct tgataattct gtttgcgcta tggtgcatca attgccctgg cagagatatc
    40261 gcaacacgcc gcttcattaa aaattcagaa tatatcaagg tattggggct cactgtcagg
    40321 aatgattatc cccaattaat caaatagtta aaaatattac gctgttattc aatgcaactc
    40381 atcaagtttt ctcatcgctt gttcccgtat tgcgcgcgtc ttaccgttaa gcgccagctg
    40441 gcggactaaa tccagttcag ggttaacaag ctctaacgcc agcaaaggat cacgtttcag
    40501 cctcgccttt aatacatcaa ctggaaaacg gggattattc atcgccacaa tccaccactc
    40561 gggatcaatt tctgccgcca gtactgcagg cggtgtgtgg cggttgttaa gtgcgccgaa
    40621 cttgaaaaat gtgagattgc ttttcgccag tgtactccac catatacgat ccggttgttt
    40681 tgccagtaca tccagttgtg ctaacgcatc gggttctagt gcataataaa cacgtaacgc
    40741 ttcgttaacc tgcgcacggg aactgttttc ctgcctcaga aaaaacttcg cccaaatatc
    40801 atttcgtggc gtgttttgct gtaattcctt taagccttgg gagagttcgc attatggcaa
    40861 gatcaaatgt cgcaatgtct cgtccttcaa atgcagcaac gttgccttca tccagcattt
    40921 gcaattcctg caataattca ggagaagact tagggcgaaa cagcgcttct tttttcatct
    40981 gattacagac attccctgct atgcgaatat tgcttcgctt aaaaatttct ttcagagcat
    41041 cttcatcatt tattaggccc caataatatg catcaggatt tattatcatc agcgcttttt
    41101 tgacattttc agtgtcattt ttgtcactcg cacggaccat gaattcacta tttgtcgaag
    41161 atgcaacaac aggtgcaatt atgtcattga taaatatccc gcgcgcatta atacttccct
    41221 taatcgtaga cggattatcg taatatgagt cgataaatac cagtccactt gcatttatcg
    41281 aaccggtaat taacagtggt gaatccgtaa ctatattcgt tgccgttacg ttacgttacg
    41341 ttacgttacg ttacgttacg ttacgttacg ttacgttacg ttaccgagaa taacccatgg
    41401 attagaaaga gagaaagtgg aaatattacc tttgactgtc aaattgccaa gaacaatcaa
    41461 tttttgatta ctctctatat taccttctac cattaaatcg ccacgatgca ctttccagtc
    41521 cgatgcagat aaaccgacgg ggacaagatt cacgctactg ttgatagctt gttcaacttg
    41581 ttgtttatct tcagcggtag ccgcagaaaa aaatggttca atatcaactg catttacaac
    41641 acatcgaagt tccgcctgta ctggaagact cagcataaac aggggaagta aacctatcca
    41701 gctgcaaatc ctgggcttaa acatatgcac tccatgccat taaaaaagaa acaaaaggga
    41761 aatgcatctt tcccttttgt atattcgtta ttctcgcgaa ataccgtcca acgtagacaa
    41821 cacttcctca gaaagatgca atttttcagc agccatattc tcccgtaaat gcgcaacaga
    41881 agacgtccct gggatcagca aaatattcgg tgaacgctgt aacagccacg ccagcgccac
    41941 ctgcattggt gttgcgccca ggctcgctgc tacatccgaa agtgtggagg attgcagcgg
    42001 cgtaaagccc ccaagcggaa agaacggcac gtaggcaatg ccgtcacgag ccaaagcatc
    42061 aatcattgcg tcatcagcac gatgagcaat gttgtattcg ttctgcacac agacgatttc
    42121 ggtaatctta cgcgcctctg caacctgcgt cggtgtaacg ttgctcagac caatatgttt
    42181 taccaggcct tgttgttgca tctctgccag cacggtcagg ctggcttcaa tcgatccttc
    42241 cgctgggcca tgaccatccc ccatcataac gcgcaggtta accacatcca gtgcgcccag
    42301 cccgagatta cgtagattat cgtgcaccgc tttttgcagc tctgccggag aaaatgcggg
    42361 caaccaggat gcgtcctctc cacgccgcgc accaatttta gtgacaattg tcaggtcgtc
    42421 agagtaagga taaagcgctt cgcggataat ctgattggtg acgtgcggac cataaaagtc
    42481 gctggtatca atatgattga cgcccaatgc cagcgcctca cgcagcacgg ttatagcgac
    42541 gtggcgatct cttggggggc caaaaacccc aggacctgcc agttgcatcg cgccataacc
    42601 aagacggtta acggattttg taccgagagt aaatgtattg ctgctcatcg caccctgccc
    42661 tgtaataaaa cggattaaga aaaatagtat agaaacgaaa agagataaat aatgcgaagg
    42721 cgtaaacaag attactggat gagttgctct attgccctga aagacaccaa aacaaaacgc
    42781 gtcaagtacc gcatccggca ataaaaaaac cgcaacctgt atcagcgttg cggtttaggt
    42841 atcacatgct aaaaattaga acgtccagca tcaggacgtt actgtttcgg tcgcagattc
    42901 tcatcaaata ccaggcgctg cctgccaggt tcaacttccc gcaatggctc aacttgatgc
    42961 aacgtctgct tactgcgtgt caccagcacc tgatactcat gcgtgccctg ctttttccat
    43021 gccagttcct gctcactaag ttcacgaatc gcttttgccg ggctgccgac aatcaggtaa
    43081 ttagcaggca tttctgcttt ggctttcaca aatgcggaag cgccaacaat gctgttctcg
    43141 ccaatcaccg caccgtccat cactaccgcg ttcataccca ctaatgcatt gcggtggata
    43201 atgcagccgt gaaggatagc gctatgacca atatgcccat cttcttccac gacagtatcc
    43261 tgatcgggaa aaccgtgcat aacgcaatta tcctgaatgt tcgcgccatc tttcaccacg
    43321 atacgaccga aatcgccacg caggctggta tttggcccaa cgtacacgcc cttgccgaga
    43381 ataacatcgc cgatcaatac cgctgtcgga tggacaaaac tctcttctgg cacaaactga
    43441 gtcagaccgt ctatctgata aattggcata acgcctcctg ttcaatattc aagccgccaa
    43501 aacgttgaaa atacaggctt cccggcgcag gcaattcacc gaccgaggtt tcaccttttt
    43561 cactaacgaa cgctaaagca gcaggcgcta cgcgctgata aagtttgccg ataaaggttt
    43621 ggtagtcgcg caatatatcc gtaaccgtcg tctcgatttt tgtgcagatg cgattcgcca
    43681 tgccgcagat gatgaaaaac tggcaggcat tggctttcat tggggatttt ccgaccagag
    43741 tcatttttca acggtattta agcaacgctt tgggatgacg ccaggcgagt atcgacgtaa
    43801 attccgctaa ctaattcgcg ttgcatgtac agcaacgcga attagttaaa acagatattt
    43861 gactatgcat ttttcagggc gaaaggcaat ttgacaggaa tttttgaata ttcacgttgt
    43921 ttccggaaaa tagattaatc gtcttctcct gtagcgttat cccgatattg cggttttgct
    43981 attaaaattt tcgcacccat ttaccgttta tcccttgtac atattccccg ggtttggccc
    44041 gttctacaag cttttgcccg gcgagtttcg cgatatcatc caccgacaca ttattctgct
    44101 ttgccagttg ttgatagctc gctttacgtt cggcattgat gtcggttact aatttctcag
    44161 tttcagcatc tgttttcagc gcaaccagat aaccataaaa tgtttcgcct acccgcccct
    44221 gagttctggc ttcatccaga gtcaatgcca ttacattgct gcttaccaag ccaacaagaa
    44281 atgcacaaag aagtaatgtt cttttcatca tcacctcaaa aaagatcgct acgggtctca
    44341 agcagctctt cgacatcttt gtctgccttg atgatgatct catgctcaat tttaacgttc
    44401 atattgatag tgatcggttc cttaggtgca gcgacttcaa tgcgaggcgt acagccaacc
    44461 agcataaaag atgacgtcaa cgcagccagt aaaattttca ttgtttatcc tcacactctt
    44521 ttccttgcgg acagtcattt cccggtagat gcgcgttctg ctccagccat gcctggagat
    44581 tatcgccaaa gcgtaaactg cgccacagcg taaaaatatt ctcttcatga tggtaattaa
    44641 gatttaccgt accgctttta ccatcaacgc gactggtacc tgtaatattg gcctgcatgg
    44701 ttaataaacc gagattatct aaattaattt ttgtcgatga acggctaatt tccatatagc
    44761 gcaaccagtt aattgctgaa cccgcggtca tattgtcttt caccaccgca tctgcggtgt
    44821 ctttatccag ccgcaaggtc attggcccgc tatttgccag ccagccgtct ttcactatcc
    44881 atttttcgtt gtttagccat aacggcaatg caccactaaa tgccccggac atggcgaatt
    44941 gtttcggatt gacggcgcta accagttcgc tggatgagag attattcagc cgcaacagcg
    45001 ccgagtcatg ttgcggcatg cgtaattgtt tcatcagcac gttaccgcct aacacatcga
    45061 cgctaacatc agtcaacagc aatggttcgt cttcactcca cggataacgc ccttgcaaat
    45121 cagccgtaat atttttcgct gtcaccagat taatcacttc ggcaattcgc aacgtaacgg
    45181 ggccgcgagt ccccagatgc caggctccat cggcaaaacg gaaaggcagg acaaaatcga
    45241 caccgttaac ctgattatct ggcatccagg cactaccgcc tttcaacaca ccgtgccctc
    45301 ccgcgcggaa tccttgttca ggcgcagctg aaaatgcaac ctgagcatac agttcgccat
    45361 cgcgtaagtt catcttccag tcgggtggca ccagcggctg gaatacagtc agtgattgtt
    45421 taggccacca ggcgttaccg cgcagacgaa taccgtccca gcgaccattt acccgaaccg
    45481 ggccaatctc accagcatgc aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat
    45541 cgacactaaa ttttaaggtt gatggcggta acacactgcc gccagtgaac agcgtttgcc
    45601 cggcgtccag tgacagcgcg ccgctaaagg agggatgctg tgcgtcacgt acccagacga
    45661 cgggcttgtc gagaattaat cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt
    45721 cgaaaccggt ggagagatcg gtcagcgtaa tcgtgctgtc atgccactca ccctttcctg
    45781 cgacatccca ggtggcattc atcggtgtaa aactgccttt tccccagtag cgccactgcc
    45841 agcggccagc gtcagggaga aaatcattcg ccagtccatc catatgcagc acgaaatcgc
    45901 ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaatg tccgtcaaca ccacgttggg
    45961 tgacttttac acccgctaaa ggccagcgga tttcatcgat gtccagcgaa tcgatgactc
    46021 ttcccttcga acgtaataac gcgcctggct caaaggccag cgttgggtca gtcagacttc
    46081 cgcttagctg cgcaggtaaa cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg
    46141 tcagctgcaa aggcagctgg ctgttatcca tacttaattt tcctgggcca aaattaagca
    46201 ccgcgttgcc cttacccgct tgcccctggg tcagcacact cagtcgtccg ctgaccagag
    46261 cgttctcgag ccctgcctgc cagttgtcga ctttgacacc cagtcgtcca ctcaaaggaa
    46321 aacctgcata cggccagctc cagcgaccat cgcttacggt caattgttgg cgagtaattt
    46381 gccacggcag atcgagcaac ggatcgccgt tatcccgtgc caccacaatc aattgcccgc
    46441 tattttcctg ccagtccagc tcggcatcca ccagtgaagg ctcctgcggc aagtttaacg
    46501 tcgcagtagc atgaccactt acaggaagtc catccggcac tagcggcata gtaaattccc
    46561 ccaccagttt aaccggcggc tgattttcaa acgcgacgac atccagttcg ctgactgtaa
    46621 gttgttgccc tttcagctgg ccttgaaatt taactttttc gccctgataa cgcagttgct
    46681 ggatatcgga ggttaatgcg agagagagtt ttccctgcca ttcctgccag ggagaaaaaa
    46741 tcagtttatc gatattgatc caggtgttag gcaacatggc ctgccactgc gcgagggttt
    46801 ttggagcggc aggcgattgc tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt
    46861 ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga aaggctggcg ttggtgatat
    46921 gcgcaagctg acaatctccc accagataac ggagatcggg aatgattaaa cctttacgcg
    46981 taatgcgtgg gctttcatct aatgcaatac gtgtcccgag cggtagccag atgcccgcca
    47041 gcgtgggaac ccacagcccg agcgtcatca gcagtgtcaa cggcacaaga ataatcagta
    47101 ataatagcgc gagaacggct ttatatttac ccagcatggg tagttaatat cctgatttag
    47161 cgaaaaatta agcattcaat acgggtattg tggcatgttt aaccgttcag ttgaaggttg
    47221 cgcctacact aagcatagtt gttgatgaat ttttcaatat cgccatagct ttcaattata
    47281 tttgaaattt tgtaaaatat ttttagtagc ttaaatgtga ttcaacatca ctggagaaag
    47341 tcttatgaaa ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt
    47401 gaacgagtcc tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc
    47461 taaaaccgcc aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccgccc
    47521 ggtgctggaa gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt
    47581 caataacgtc gaccttgacg cggcaaaaga actggggctg aaagtagtcc gtgttccagc
    47641 ctatgatcca gaagccgttg ctgaacacgc catcggtatg atgatgacgc tgaaccgccg
    47701 tattcaccgc gcgtatcagc gtacccgtga cgccaacttc tctctggaag gtctgaccgg
    47761 ctttactatg tatggcaaaa cggcaggcgt tatcggtacc ggcaaaatcg gtgtggcgat
    47821 gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc
    47881 ggcggcgctg gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga
    47941 cgttatctct ctgcactgcc cgctgacacc ggaaaactat catctgttga acgaagctgc
    48001 cttcgaacag atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga
    48061 ttctcaggca gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt
    48121 gtatgagaac gaacgcgatc tgttctttga agataaatcc aacgacgtaa ttcaggatga
    48181 cgtattccgt cgcctgtctg cctgccacaa cgtgctgttt accgggcatc aggcattcct
    48241 gacagcagaa gcgctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga
    48301 aaaaggcgaa acctgcccga acgaactggt ttaatcttgc cgctcccctg cattccaggg
    48361 gagctgattc agataatccc caatgacctt tcatcctcta ttcttaaaat agtcctgagt
    48421 cagaaactgt aattgagaac cacaatgaag aaagtagccg cgctcgttgc gctaagcctg
    48481 ctgatggcgg gatgtgtaag taatgacaaa attgctgtaa ccccagaaca gctacagcat
    48541 catcgctttg tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca
    48601 gaaatcagct ttggtgaaaa gatgatgatt tccggcagca tgtgtaaccg ctttagcggt
    48661 gaaggcaaac tgtctaatgg tgaactgacc gccaaagggc tggcaatgac ccgtatgatg
    48721 tgcgctaacc cgcagcttaa tgaactcgat aacaccatta gcgaaatgct gaaagaaggt
    48781 gcacaagtgg atctgaccgc gaaccagtta acgctggcga ccgcgaaaca gacattaact
    48841 tataagctgg cggatttaat gaattaatag ctgccacagc tcccggcggc aagtgactgt
    48901 tcgctacagc gtttgccgtt gggtaatgca cacatcccaa tcgtcgtacc atccagttga
    48961 cgggcaacag aaagcgaacc gccgatcatt gcacaatttg cttctccact actggacatc
    49021 gacgctttta aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg ttcactacta
    49081 cacgccgaca acaataaagc ggcacaccct acccaaaacg ctgctcgcat cttttcttcc
    49141 tctgatcttc aagccaaacg acaccgccat aaataatagg cagcacagag ggcggcgtcg
    49201 agagctgtcc tgagcgttgc cccgcatttt tactttttta tggctatttt tttgccctct
    49261 gtttgatcaa aacattcatt acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa
    49321 agccatttgc taaaattgaa ggattactac tgggcgcgca gcaatttcgt gcgcccctca
    49381 tttgcgcaat gtaagggtgt catatgatta ctattgacgg taatggcgcg gttgcttcgg
    49441 tggcgtttcg caccagtgaa gttatcgcca tctaccctat tacccccagt tccacgatgg
    49501 cagaacaggc tgatgcctgg gccggaaacg gcttgaagaa cgtttgggga gacacaccac
    49561 gcgtggttga aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga
    49621 cgggtgccct ctcaacatcg tttacgtcat cgcagggttt gctgctgatg atcccgacgc
    49681 tgtacaaact ggcaggcgaa ctgacgccat ttgtcctgca tgtagcggca cgtaccgttg
    49741 ccacacatgc actctctatt tttggcgatc attccgacgt tatggcggtg cgccagacgg
    49801 gttgcgcgat gttgtgtgcg gctaacgtcc aggaagcgca agactttgct ctcatttcgc
    49861 acatcgcaac gctgaagagc cgcgtgccat ttattcattt ctttgatggt ttccgcacgt
    49921 cccacgaaat caataaaatt gtcccgctgg ccgatgacac tattcttgat ctcatgccgc
    49981 aggccgaaat tgatgctcat cgcgcccggg cgctcaaccc ggaacatccg gtgatccgcg
    50041 gtacgtccgc caatcctgac acttatttcc agtctcgcga agccaccaac ccatggtaca
    50101 acgcagtcta tgaccatgtt gaacaggcga tgaatgattt cgctgccgcg acaggtcgcc
    50161 agtatcagcc gtttgagtat tacgggcatc cacaagcgga acgggtgatt atcctgatgg
    50221 gctctgccat tggcacctgt gaagaagtgg ttgatgaatt gctgacccgt ggcgaaaaag
    50281 tcggcgtgct gaaagttcgc ctgtaccgcc ccttctccgc caaacactta ctgcaagcgt
    50341 tgccggactc tgttcggaca gtggcggtgc tggatcgcac caaagagcca ggtgcgcaag
    50401 cggaacccct gtacctggat gtgatgaccg cactggcaga agcctttaat aatggcgagc
    50461 gtgaaaccct gccccgtgtc attggtgggc gctatggtct ttcatccaaa gaatttggcc
    50521 cggactgtgt actggcggta tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta
    50581 cggtaggtat ttacgatgat gtgaccaatc tgtcactgcc gttgccggaa aacaccctgc
    50641 caaactcggc gaaacttgaa gccttgtttt atggccttgg tagtgatggc agcgtttccg
    50701 cgaccaaaaa caatatcaag attatcggta attccacgcc gtggtacgca cagggctatt
    50761 ttgtttacga ctccaaaaag gcgggcggcc tgacggtttc tcaccttcgc gtgagcgagc
    50821 agccgattcg ttccgcttat ctgatttccc aggctgattt tgttggctgc caccagttgc
    50881 agtttatcga taaatatcag atggctgaac gtttaaaacc tggcggcatt ttcctgctca
    50941 acacgccgta cagcgtagat gaagtatggt cgcgcttgcc gcaggaagtt caggccgtgt
    51001 taaaccagaa aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg
    51061 gtctggcggc ccgtattaat accgtcatgc agatggcttt tttccatctg acgcaaattc
    51121 tgcctggcga tagcgccctc gcagaattgc agggtgcgat tgccaaaagt tacagtagca
    51181 aaggtcagga tctggtggag cgcaactggc aggctctggc gctggcgcgt gaatccgtag
    51241 aagaagttcc gttgcagccg gttaatccgc acagcgccaa tcgaccgcct gtggtttccg
    51301 atgccgctcc tgatttcgtg aaaaccgtta ccgcggcgat gctcgccggg cttggtgatg
    51361 ccctccccgt ttcggcgctg ccgccagacg gcacctggcc gatgggcacc acgcgctggg
    51421 aaaaacgcaa tatcgccgaa gagatcccca tctggaaaga ggaactctgt acccaatgta
    51481 accactgcgt cgctgcttgc ccacattcgg ctattcgcgc aaaagtggta ccgcctgaag
    51541 cgatggaaaa cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg
    51601 ggcagaaata tgtcttgcag gtggcaccgg aagattgcac cggctgtaac ctgtgcgtcg
    51661 aagtttgccc ggcgaaagac cgtcagaatc cagagattaa agccatcaat atgatgtcgc
    51721 gcctggaaca tgtcgaagaa gagaaaatca attacgattt cttcctcaac ctcccagaaa
    51781 tcgaccgtag caaactggaa cgtattgata ttcgtacatc acagctgatt acaccgctgt
    51841 ttgaatattc aggtgcttgc tccggttgtg gcgagacgcc gtatattaaa ttgctgactc
    51901 agctctatgg cgaccgaatg ttgatcgcga acgccaccgg ctgttcttca atttatggcg
    51961 gtaacctgcc ctctacaccg tatactaccg atgccaacgg tcgtggaccg gcatgggcga
    52021 actcgctgtt tgaagataac gccgaatttg gccttggttt ccgcctgacg gtcgatcaac
    52081 accgtgtccg cgtgttgcgt ctgctggatc aatttgccga taaaatcccg gcggaattac
    52141 taactgcgtt gaaatcagac gccacgccag aggttcgtcg tgaacaggtt gcagctttac
    52201 gccagcaact caaagatgtt gccgaagcac atgaactgct acgtgacgca gatgcactgg
    52261 tagaaaaatc aatctggctg attggtggtg atggctgggc ttacgatatc ggctttggcg
    52321 gtctggatca tgtattgagt ttgacggaaa acgtcaacat tctggtgctg gatacgcaat
    52381 gctattccaa caccggtggt caggcgtcga aagcgacacc gctgggtgca gtaactaagt
    52441 tcggcgagca cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg
    52501 gtcatgttta tgtggcgcag atttctctcg gcgcgcagct caaccagacg gtgaaagcga
    52561 ttcaggaagc ggaagcgtat ccggggccat cgctgatcat cgcttatagc ccctgtgaag
    52621 agcatggtta cgatctggca ctcagtcacg accagatgcg ccaactcact gccaccggct
    52681 tctggccgct atatcgcttc gatccacgtc gagccgatga aggcaaactg ccgctggcat
    52741 tggattcacg cccgccgtca gaagcactgg aagaaacgtt acttcacgag cagcgtttcc
    52801 gtcgtctgaa ttcgcagcag ccagaagtgg cagaacagtt atggaaagat gctgcagctg
    52861 atttgcaaaa acgctatgac ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca
    52921 ccgattaaat gcactggata aggattatcc aattctaaaa aaaagcccgg acgactgttc
    52981 gggcttgtct ttttatatgt tggaaaatca gtagcaatgc aatggcccaa cagaaaataa
    53041 aatctgtgac aataaaggca t
//
LOCUS       DAZKJR010000013        52817 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKJR010000013 DAZKJR010000000
VERSION     DAZKJR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.013"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..979
                     /gene="pfkB"
                     /locus_tag="ACHOWS_000808"
     CDS             50..979
                     /gene="pfkB"
                     /locus_tag="ACHOWS_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFQ0046436.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPVGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1080..1370
                     /gene="ghoS"
                     /locus_tag="ACHOWS_000809"
     CDS             1080..1370
                     /gene="ghoS"
                     /locus_tag="ACHOWS_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFQ0046437.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            1476..2336
                     /gene="yniA"
                     /locus_tag="ACHOWS_000810"
     CDS             1476..2336
                     /gene="yniA"
                     /locus_tag="ACHOWS_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0046438.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2377..2913)
                     /gene="yniB"
                     /locus_tag="ACHOWS_000811"
     CDS             complement(2377..2913)
                     /gene="yniB"
                     /locus_tag="ACHOWS_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFQ0046439.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFFLIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3060..3728
                     /gene="hxpB"
                     /locus_tag="ACHOWS_000812"
     CDS             3060..3728
                     /gene="hxpB"
                     /locus_tag="ACHOWS_000812"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFQ0046440.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYIDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3892..4482
                     /gene="ydjM"
                     /locus_tag="ACHOWS_000813"
     CDS             3892..4482
                     /gene="ydjM"
                     /locus_tag="ACHOWS_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0046441.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4615..6006
                     /gene="tcyP"
                     /locus_tag="ACHOWS_000814"
     CDS             4615..6006
                     /gene="tcyP"
                     /locus_tag="ACHOWS_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFQ0046442.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6053..6316)
                     /gene="cedA"
                     /locus_tag="ACHOWS_000815"
     CDS             complement(6053..6316)
                     /gene="cedA"
                     /locus_tag="ACHOWS_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFQ0046443.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            6499..8760
                     /gene="katE"
                     /locus_tag="ACHOWS_000816"
     CDS             6499..8760
                     /gene="katE"
                     /locus_tag="ACHOWS_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFQ0046444.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(9018..9776)
                     /gene="chbG"
                     /locus_tag="ACHOWS_000817"
     CDS             complement(9018..9776)
                     /gene="chbG"
                     /locus_tag="ACHOWS_000817"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFQ0046445.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSLDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGCYRDV"
     gene            complement(9789..11141)
                     /gene="celF"
                     /locus_tag="ACHOWS_000818"
                     /gene_synonym="chbF"
     CDS             complement(9789..11141)
                     /gene="celF"
                     /locus_tag="ACHOWS_000818"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0046446.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIADLKKAH
                     "
     gene            complement(11245..12087)
                     /gene="chbR"
                     /locus_tag="ACHOWS_000819"
     CDS             complement(11245..12087)
                     /gene="chbR"
                     /locus_tag="ACHOWS_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFQ0046447.1"
                     /translation="MMQPVINALEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(12095..12445)
                     /gene="chbA"
                     /locus_tag="ACHOWS_000820"
     CDS             complement(12095..12445)
                     /gene="chbA"
                     /locus_tag="ACHOWS_000820"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFQ0046448.1"
                     /translation="MMDLDNIPDTQTEVEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKTMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(12496..13854)
                     /gene="chbC"
                     /locus_tag="ACHOWS_000821"
     CDS             complement(12496..13854)
                     /gene="chbC"
                     /locus_tag="ACHOWS_000821"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFQ0046449.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWTLNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(13939..14259)
                     /gene="chbB"
                     /locus_tag="ACHOWS_000822"
     CDS             complement(13939..14259)
                     /gene="chbB"
                     /locus_tag="ACHOWS_000822"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFQ0046450.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(14558..14896)
                     /gene="osmE"
                     /locus_tag="ACHOWS_000823"
     CDS             complement(14558..14896)
                     /gene="osmE"
                     /locus_tag="ACHOWS_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFQ0046451.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            15098..15925
                     /gene="nadE"
                     /locus_tag="ACHOWS_000824"
     CDS             15098..15925
                     /gene="nadE"
                     /locus_tag="ACHOWS_000824"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0046452.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            16155..17042
                     /gene="cho"
                     /locus_tag="ACHOWS_000825"
     CDS             16155..17042
                     /gene="cho"
                     /locus_tag="ACHOWS_000825"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFQ0046453.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(17002..17577)
                     /gene="ves"
                     /locus_tag="ACHOWS_000826"
     CDS             complement(17002..17577)
                     /gene="ves"
                     /locus_tag="ACHOWS_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFQ0046454.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(17780..18265)
                     /gene="spy"
                     /locus_tag="ACHOWS_000827"
     CDS             complement(17780..18265)
                     /gene="spy"
                     /locus_tag="ACHOWS_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFQ0046455.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(18595..19563)
                     /gene="astE"
                     /locus_tag="ACHOWS_000828"
     CDS             complement(18595..19563)
                     /gene="astE"
                     /locus_tag="ACHOWS_000828"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFQ0046456.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(19556..20899)
                     /gene="astB"
                     /locus_tag="ACHOWS_000829"
     CDS             complement(19556..20899)
                     /gene="astB"
                     /locus_tag="ACHOWS_000829"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFQ0046457.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAS
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(20896..22374)
                     /gene="astD"
                     /locus_tag="ACHOWS_000830"
     CDS             complement(20896..22374)
                     /gene="astD"
                     /locus_tag="ACHOWS_000830"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0046458.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVMAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGRWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(22371..23405)
                     /gene="astA"
                     /locus_tag="ACHOWS_000831"
     CDS             complement(22371..23405)
                     /gene="astA"
                     /locus_tag="ACHOWS_000831"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFQ0046459.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEESETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLAANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(23402..24622)
                     /gene="astC"
                     /locus_tag="ACHOWS_000832"
     CDS             complement(23402..24622)
                     /gene="astC"
                     /locus_tag="ACHOWS_000832"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFQ0046460.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFAYRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRCGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSGGSS"
     gene            25068..25874
                     /gene="xthA"
                     /locus_tag="ACHOWS_000833"
     CDS             25068..25874
                     /gene="xthA"
                     /locus_tag="ACHOWS_000833"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0046461.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTANRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            26041..26751
                     /gene="ydjX"
                     /locus_tag="ACHOWS_000834"
     CDS             26041..26751
                     /gene="ydjX"
                     /locus_tag="ACHOWS_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFQ0046462.1"
                     /translation="MNAERKFLFACLIFALVIYAFHAFGLFELLTDLPHLLTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            26756..27433
                     /gene="ydjY"
                     /locus_tag="ACHOWS_000835"
     CDS             26756..27433
                     /gene="ydjY"
                     /locus_tag="ACHOWS_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFQ0046463.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            27447..28154
                     /gene="ydjZ"
                     /locus_tag="ACHOWS_000836"
     CDS             27447..28154
                     /gene="ydjZ"
                     /locus_tag="ACHOWS_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFQ0046464.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            28154..28702
                     /gene="ynjA"
                     /locus_tag="ACHOWS_000837"
     CDS             28154..28702
                     /gene="ynjA"
                     /locus_tag="ACHOWS_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0046465.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SIFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLVTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            28712..29878
                     /gene="ynjB"
                     /locus_tag="ACHOWS_000838"
     CDS             28712..29878
                     /gene="ynjB"
                     /locus_tag="ACHOWS_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0046466.1"
                     /translation="MRHCVWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYDINLKIVHLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQFLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            29851..31386
                     /gene="ynjC"
                     /locus_tag="ACHOWS_000839"
     CDS             29851..31386
                     /gene="ynjC"
                     /locus_tag="ACHOWS_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0046467.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAVLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQAKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLTATFIALILIL
                     AWLEWGSSRRHFWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            31386..32039
                     /gene="ynjD"
                     /locus_tag="ACHOWS_000840"
     CDS             31386..32039
                     /gene="ynjD"
                     /locus_tag="ACHOWS_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0046468.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGGLAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            32106..33413
                     /gene="ynjE"
                     /locus_tag="ACHOWS_000841"
     CDS             32106..33413
                     /gene="ynjE"
                     /locus_tag="ACHOWS_000841"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFQ0046469.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKKRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDIEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(33422..34042)
                     /gene="ynjF"
                     /locus_tag="ACHOWS_000842"
     CDS             complement(33422..34042)
                     /gene="ynjF"
                     /locus_tag="ACHOWS_000842"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0046470.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALIVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            34129..34536
                     /gene="nudG"
                     /locus_tag="ACHOWS_000843"
     CDS             34129..34536
                     /gene="nudG"
                     /locus_tag="ACHOWS_000843"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFQ0046471.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(34502..34774)
                     /gene="ynjH"
                     /locus_tag="ACHOWS_000844"
     CDS             complement(34502..34774)
                     /gene="ynjH"
                     /locus_tag="ACHOWS_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HFQ0046472.1"
                     /translation="MSRALFAVVLAFPIIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQQDDKNLSTNPLVWRRVKP"
     gene            35010..36353
                     /gene="gdhA"
                     /locus_tag="ACHOWS_000845"
     CDS             35010..36353
                     /gene="gdhA"
                     /locus_tag="ACHOWS_000845"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0046473.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(36430..38385)
                     /gene="topB"
                     /locus_tag="ACHOWS_000846"
     CDS             complement(36430..38385)
                     /gene="topB"
                     /locus_tag="ACHOWS_000846"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0046474.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(38390..39433)
                     /gene="selD"
                     /locus_tag="ACHOWS_000847"
     CDS             complement(38390..39433)
                     /gene="selD"
                     /locus_tag="ACHOWS_000847"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFQ0046475.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRHACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRELLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(39550..40101)
                     /gene="ydjA"
                     /locus_tag="ACHOWS_000848"
     CDS             complement(39550..40101)
                     /gene="ydjA"
                     /locus_tag="ACHOWS_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFQ0046476.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAAMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            40262..42118
                     /gene="sppA"
                     /locus_tag="ACHOWS_000849"
     CDS             40262..42118
                     /gene="sppA"
                     /locus_tag="ACHOWS_000849"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0046477.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            42283..43299
                     /gene="ansA"
                     /locus_tag="ACHOWS_000850"
     CDS             42283..43299
                     /gene="ansA"
                     /locus_tag="ACHOWS_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0046478.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            43310..43951
                     /gene="pncA"
                     /locus_tag="ACHOWS_000851"
     CDS             43310..43951
                     /gene="pncA"
                     /locus_tag="ACHOWS_000851"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFQ0046479.1"
                     /translation="MHPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(44112..44384)
                     /gene="yeaC"
                     /locus_tag="ACHOWS_000852"
     CDS             complement(44112..44384)
                     /gene="yeaC"
                     /locus_tag="ACHOWS_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFQ0046480.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(44426..44839)
                     /gene="msrB"
                     /locus_tag="ACHOWS_000853"
     CDS             complement(44426..44839)
                     /gene="msrB"
                     /locus_tag="ACHOWS_000853"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0046481.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            45181..46176
                     /gene="gapA"
                     /locus_tag="ACHOWS_000854"
     CDS             45181..46176
                     /gene="gapA"
                     /locus_tag="ACHOWS_000854"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0046482.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            46260..47162
                     /gene="yeaD"
                     /locus_tag="ACHOWS_000855"
     CDS             46260..47162
                     /gene="yeaD"
                     /locus_tag="ACHOWS_000855"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFQ0046483.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGPAAQQGLPAHG
                     FARNLPWTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKACEIDLEAH
                     GEFETTSALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYL
                     NPQDCSVINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAY
                     ASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(47213..48067)
                     /gene="yeaE"
                     /locus_tag="ACHOWS_000856"
     CDS             complement(47213..48067)
                     /gene="yeaE"
                     /locus_tag="ACHOWS_000856"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFQ0046484.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(48157..48903)
                     /gene="mipA"
                     /locus_tag="ACHOWS_000857"
     CDS             complement(48157..48903)
                     /gene="mipA"
                     /locus_tag="ACHOWS_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFQ0046485.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYDGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            49339..51273
                     /gene="yeaG"
                     /locus_tag="ACHOWS_000858"
     CDS             49339..51273
                     /gene="yeaG"
                     /locus_tag="ACHOWS_000858"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFQ0046486.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            51386..52669
                     /gene="yeaH"
                     /locus_tag="ACHOWS_000859"
     CDS             51386..52669
                     /gene="yeaH"
                     /locus_tag="ACHOWS_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFQ0046487.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        12969 a        13761 c        13095 g        12992 t
ORIGIN      
        1 ctttccgctg attcggtgcc agactgaaat cagcctatag gaggaaatga tggtacgtat
       61 ctatacgttg acacttgcgc cctctctcga tagcgcaaca attaccccgc aaatttatcc
      121 cgaaggaaag ctgcgctgta ccgcaccggt attcgaaccc ggaggcggcg gcatcaacgt
      181 cgcccgcgcc attgcccatc ttggaggcag tgctacagcg atcttcccgg tgggaggtgc
      241 gaccggcgaa catctggttt cactgttggc agatgaaaat gtccccgtcg ctactgtaga
      301 agccaaagac tggacccggc agaatttaca cgtacatgtg gaagcaagcg gtgagcagta
      361 tcgttttgtc atgccaggcg cggcattaaa tgaagatgag tttcgccagc ttgaagagca
      421 agttctggaa attgaatccg gtgctatcct ggtcataagc ggaagcctgc cgccaggtgt
      481 gaagctggaa aaattaaccc aactgatttc cgctgcgcaa aaccaaggga tccgctgtat
      541 cgtcgacagt tctggcgaag cgttaagtgc agcactggcg attggtaaca tcgagttggt
      601 taagcctaac caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc agccagacga
      661 tgtccgcaaa gccgcgcagg aaatcgttaa tagcggcaag gccaaacgag ttgtcgtttc
      721 cctgggtcca caaggggcgc tgggcgttga tagtgaaaac tgcattcagg ttgtgccacc
      781 accggtgaaa agccagagca ccgttggcgc tggtgacagc atggtcggcg cgatgacact
      841 gaaactggca gaaaatgcct ctcttgaaga gatggtccgt tttggcgtag ctgcggggag
      901 tgcagccaca ctcaatcagg gaacacgcct gtgctcccat gacgatacac aaaaaattta
      961 cgcttacctt tcccgctaac tgaaacattc ccccagcatt gggggaatca ccaccaacct
     1021 gtcggcaacg cgtttctccg actatgctca aaagtcatgt gataacaaag gggtgaacta
     1081 tggccagtgg cgatcttgtc cgttatgtca taaccgtcat gttgcacgaa gatacattga
     1141 ctgaaattaa cgagttgaat aattacctga ctcgcgacgg ttttttgctc accatgacgg
     1201 atgatgatgg aaatatccat gagctgggga ctaatacttt tggacttatc agtacccaaa
     1261 gtgaagaaga aattagagaa cttgtttcgg ggcttaccca aagtgcaacc ggtaaagatc
     1321 ctgaaatagc catcacgacc tgggaggaat ggaatagcaa cagaaaataa atggtttttg
     1381 ggcaataatc agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt cttgcgctaa
     1441 ccttatgatc tggcagacaa catgggagag acatcatgtg gcaggcaatc agtcgtcttt
     1501 tgagcgagca gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct ggcggagaag
     1561 tccatgccgc atggcatttg cgctatgcag gacatgactt tttcgtcaaa tgtgatgaaa
     1621 gggaactgct tcccggtttt accgccgaag ccgaccaact ggagttactg tcgcgtagta
     1681 aaaccgtcac cgtgcctaag gtttgggcag ttggcgctga ccgtgactac agttttctgg
     1741 tgatggatta tctcccacct cgcccgctgg atgcgcatag cgcatttatt cttggtcagc
     1801 aaattgcgcg tttacatcaa tggagcgacc aaccgcaatt tggcctcgat ttcgataacg
     1861 cgctctccac aactccccag cccaacacct ggcaacgtcg ctggtcaact ttttttgctg
     1921 aacaacggat tggctggcag ctggaactgg cggcagaaaa agggatcgct ttcggcaata
     1981 tcgatgccat cgtcgagcat attcagcagc gtctggcctc gcatcaaccg cagccttctc
     2041 tgttgcacgg cgatttatgg tccggcaact gtgcgctggg tccggatggc ccgtacattt
     2101 tcgacccggc ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta ccgctgcata
     2161 ctgaacagcc gccacaaatc tatgacggct atcagtcagt atccccgcta cctgccgatt
     2221 tccttgaacg tcaaccggtt taccaactct acacgctgtt aaatcgtgca aggttatttg
     2281 gcggtcagca tttggttatt gctcagcagt cactggatag attattagca gcataatatg
     2341 gggtgaggat aatggccgct ccgtgcggcc ttttgattaa ataaacccca gcagagaaaa
     2401 gaacacataa cctgcagcaa tacaaataac cggcaggata tacagcgaga aaaactgcag
     2461 gaaaatagta tggtgcggca caacaatacg tgactcaatt tgttcacgcg ttagcccctc
     2521 ttcccctttg gctttttcca gaatgagttg atcttcaaca ccttcccgca agaagcgcgc
     2581 ctggcgactc atccgggcac cggaatcctg caacgccagt ccgacaaaaa tcaggaagaa
     2641 aatcacccag aacataacgt tcacgccacc attaaaattg ggcgtcgggg agttatacca
     2701 gaacagattc aaaaaaggcg tattcgcctg catcatatcg atcatgacat gagtaaagtc
     2761 gagcatgacc gcattaatgc cttcctggtt ttcctgccgg gtattcataa atttaagcaa
     2821 agaaatcaat gtagagatca atgcaggtat aaaaatcacc caacccaaaa tccttttcaa
     2881 aacagcaatg cgtccagctt gttgatacgt catgagttct ccttgattaa gacgcgtcgt
     2941 ttcacttagt ttacctgtag atatctgttt tcgcccattc tttaaaggcg atatgatagg
     3001 cgcttaatca taagcacggc ttaatacctt acatataatg ctctaaagga gaggttgtaa
     3061 tgtcaacccc gcgtcagatt cttgctgcaa tttttgatat ggatggatta cttatcgact
     3121 cagaaccttt atgggatcga gccgaactgg atgtgatggc aagcctgggg gtggatatct
     3181 cccgtcgtaa cgagctgccg gacaccttag gtttacgcat cgatatggtg gtcgatcttt
     3241 ggtacgcccg gcaaccatgg aatgggccaa gccgtcagga agtagtagaa caagttattg
     3301 ctcgtgccat ttcactggtt gaagagacgc gtccattatt accaggtgtg cgtgaagccg
     3361 ttgcgttatg caaagaacaa ggtttattgg tggggctggc ctccgcgtca ccgctgcata
     3421 tgctggaaaa agtattgacc atgtttgatt tacgcgacag tttcgatgcc ctcgcctcgg
     3481 ctgaaaaatt gccttacagc aagccgcatc cgcaagtata tatcgactgc gcagcaaaac
     3541 tgggggttga ccctctgacc tgcgtggcac tggaagattc ggtaaatggc atgatcgcct
     3601 ctaaagcagc ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa aatgatccac
     3661 gttttgtatt agcaaacgtc aaactctcat cgctgacaga actcaccgca aaagaccttc
     3721 tcggttaatg accagggggc agtgatcaca ctgcccctgg ttctttatct gaatttgcat
     3781 tcaactgacg gattaatcgc caatttaaga gaaagagtta caccgtcacc acttccgtgc
     3841 actgtataaa aaccctatac tgtacgtatc gacagtttag tgagttttat catgacggcg
     3901 gaaggtcacc ttctcttttc tattgcttgt gcggtatttg ccaaaaatgc cgagctgacg
     3961 cccgtgctgg cacagggtga ctggtggcat attgtccctt ccgcaatcct gacgtgtttg
     4021 ttaccggaca tcgatcaccc aaagtcgttt cttgggcagc gattaaagtg gatatcaaaa
     4081 ccaatcgccc gcgcttttgg gcatcgtggt tttacccaca gtctgctggc ggtatttgcg
     4141 ctgctggcaa ccttttacct taaggttcct gaaagctggt tcattccggc tgatgcgcta
     4201 caaggaatgg tgctgggtta tttgagccac atacttgccg atatgctgac acccgccggt
     4261 gttcccctgc tctggccatg ccgctggcgt ttccgcttgc ctatcctggt tccccaaaag
     4321 ggcaaccaac tggaacgttt tatctgcatg gcattatttg tctggtcggt atggatgccc
     4381 cattcattac ccgaaaacag cgctgtccgt tggtcatcgc aaatgatcaa taccttgcag
     4441 atccagtttc atcggcttat taagcatcag gttgaatact aaaaaggcaa aaatcacctt
     4501 tctggaataa gcaattccat ttgaatataa gagccagctc acagttctgt taatcttgcg
     4561 ccaacactat gactgctacg cagtgataga aataataaga tcaggagaac ggggatgaac
     4621 tttccattaa ttgcgaacat cgtggtgttc gttgtactgc tgtttgcgct ggctcagacc
     4681 cgccataaac agtggagtct ggcgaaaaaa gtgctggtgg gtctggtgat gggtgtggtt
     4741 tttggcctcg ccctgcatac catttatggt tctgacagcc aggtacttaa agattctgta
     4801 cagtggttta acattgttgg taacggctat gttcaactcc tgcaaatgat cgttatgccg
     4861 ttagtctttg cctctattct gagcgcggtt gcccgtctgc ataacgcatc acagttaggc
     4921 aaaatcagtt ttctgaccat cggaacgctt ttgtttacca cactgattgc ggcgctggtc
     4981 ggtgtgctgg tcaccaacct gtttggtttg acggctgaag gtctggttca gggtggtgca
     5041 gaaactgcac gtttgaacgc catcgaaagt aactatgttg gtaaagtctc tgacctgagc
     5101 gttccgcagc tggtcttgtc ctttatcccg aaaaacccgt ttgccgattt gaccggagcc
     5161 aatccgacgt caattatcag cgtggtaatt tttgccgcat tcctcggcgt agctgcgctg
     5221 aagctgctga aggatgatgc gccgaaaggt gaacgcgtct taaccgctat cgataccctg
     5281 caaagctggg tgatgaaact ggttcgcctg gtcatgcagc tgacccctta cggcgttctg
     5341 gcgctgatga ccaaagtggt tgcaggttct aacctgcaag acatcatcaa actgggaagt
     5401 ttcgttgtcg cgtcctacct cggtcttctg attatgtttg cagtgcatgg ccttctgctg
     5461 ggtattaatg gcgtgagccc gctgaagtac ttccgtaagg tatggccagt actgacgttt
     5521 gcctttacca gccgttccag tgctgcgtct atcccactga atgtggaagc acaaacgcgt
     5581 cgactcggtg ttcctgaatc catcgccagt ttcgccgcct ctttcggtgc aaccattggt
     5641 cagaacggct gtgccggttt gtatccggcg atgctggcgg tgatggttgc gcctacggtt
     5701 ggcattaacc cgatggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     5761 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     5821 gcgatgggcc tgccggtaac gctggtggca ttgttaatct ccgttgaacc gcttatcgac
     5881 atgggtcgta cggcgctaaa cgttagtggc tcgatgaccg ctggcacgct gaccagccag
     5941 tggctgaagc aaaccgataa agctattctg gatagcgaag acgacgccga actggcacac
     6001 cgttaatcct gatttgttac gttaaacgcc tgtcatcccg acaggtgttt ttttattctt
     6061 cgacctcatt ttcctgacga atctgatttg cccaacgctg ggcggattca ggcacagtaa
     6121 acgccggtga gcgcagaaat gactctccca tcagtacaaa cgcaacatat ttgccacgca
     6181 gcatccagac atcacgaaac gaatccatct ttatcgcatg ttctggcggc gcgggttccg
     6241 tgcgtgggac atagctaata atctggcggt tttgctggcg gagcggtttc ttcattactg
     6301 gcttcactaa acgcatatta aaaatcagaa aaactgtagt ttagccgatt tagcccctgt
     6361 acgtcccgct ttgcgtgtat ttcataacac cgtttccaga atagtctccg aagcgggatc
     6421 tggctgatgg tctatagtta gagggttttt tgaccaaaac agcggccctt tcagtaataa
     6481 tttaaggaga cgagttcaat gtcgcaacat aacgaaaaga acccacatca gcaccagtca
     6541 ccactacacg attccagcga agcgaaaccg gggatggact cactggcacc tgaagacgga
     6601 tctcatcgtc cagcggctga accaacaccg cctggtgcac aacctaccgc cccagggagc
     6661 ctgaaagccc ctgatacgcg taacgaaaaa cttaattctc tggaagacgt acgcaaaggc
     6721 agtgaaaatt atgcgctgac cactaatcag ggcgtgcgca tcgccgacga tcaaaactca
     6781 ctgcgtgccg gtagccgtgg tccaactctg ctggaagatt ttattctgcg cgagaaaatc
     6841 actcactttg accatgagcg catcccggaa cgtattgttc atgcacgcgg atcagcagct
     6901 cacggttatt tccagccata taaaagctta agcgatatca ccaaagcaga tttcctctca
     6961 gatccgaaca aaatcacccc agtatttgta cgtttctcta ccgttcaggg tggtgctggc
     7021 tctgccgata ccgtgcgtga tatccgtggc tttgccacca agttctatac cgaagagggt
     7081 atttttgacc tcgttggcaa taacacgcca atcttcttta tccaggatgc gcataaattc
     7141 cccgattttg ttcatgcggt aaaaccagaa ccgcactggg caattccaca agggcaaagc
     7201 gcccacgata ctttctggga ttatgtttct ctgcaacctg aaactctgca caacgtgatg
     7261 tgggcgatgt cggatcgcgg gatcccgcgc agttaccgca ccatggaagg cttcggtatt
     7321 cacaccttcc gcctgattaa tgccgaaggg aaggcaacgt tcgtacgttt ccactggaaa
     7381 ccactggcag gtaaagcctc actcgtttgg gatgaagcac aaaaactaac cggacgtgac
     7441 ccggacttcc accgccgcga gttgtgggaa gccatcgaag caggcgattt tccggaatac
     7501 gaactgggct tccagttgat tcctgaggaa gatgaattta agttcgactt cgaccttctt
     7561 gatccaacta aacttatccc ggaagaactg gtgcccgttc agcgtgtcgg caagatggtg
     7621 ctcaatcgca atccggataa cttctttgcc gaaaacgaac aagcagcatt ccatccaggt
     7681 catattgttc ctggtctgga cttcaccaac gatcccctgt tgcagggacg tttgttctcc
     7741 tataccgata cacaaatcag tcgtcttggt gggccgaatt tccatgagat tccgattaac
     7801 cgcccgacct gcccttacca taatttccag cgtgacggca tgcatcgtat ggggatcgac
     7861 actaacccgg cgaattatga accgaactcg atcaacgata actggccgcg cgaaacaccg
     7921 ccggggccga aacgcggcgg ttttgaatca taccaggagc gcgtggaagg caataaagtt
     7981 cgcgagcgca gcccatcgtt tggcgaatat tattcccatc cgcgtctgtt ctggctaagt
     8041 cagacgccat tcgagcagcg ccatattgtc gatggtttca gttttgagtt aagcaaagtc
     8101 gttcgtccgt atattcgtga gcgcgttgtt gaccagctgg cacatattga tctcactctg
     8161 gcccaggcgg tggcgaaaaa tctcggtatc gagctgacgg acgaccagct gaatatcacc
     8221 ccgcctccgg acgtcaacgg tctgaaaaag gatccatcct taagtttgta cgccattcct
     8281 gacggtgatg tgaaaggtcg cgtggtagcg attttactta atgatgaagt gagatcggca
     8341 gaccttctgg ccattctcaa ggcgctgaag gccaaaggcg ttcatgccaa actgctctac
     8401 tcccgaatgg gtgaagtgac tgcggatgac ggtacggtgt tgcctatagc cgctaccttt
     8461 gccggtgcac cttcgctgac ggtcgatgcg gttattgtcc cttgcggcaa tatcgcggat
     8521 atcgctgaca acggcgatgc caactactac ctgatggaag cctacaaaca ccttaaaccg
     8581 attgcgctgg caggagacgc gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt
     8641 gaagaaggga ttgtggaagc tgacagcgcc gacggtagtt ttatggatga actgttaacg
     8701 ctgatggcag cacaccgcgt gtggtcacgt attcctaaga ttgacaaaat tcctgcctga
     8761 tgggaggcgg cgcagttgcg ccgcctcaaa tgatttacat agtgcgcttt gtttatgtcg
     8821 gatgcggcgt gaacgcctta tccggcctac aaaactgtgc aaattcaata tattgcagga
     8881 aaaacgtagg cctgataagc gaagcgcatc aggcagtttt gcgtttgtca gcagtctcaa
     8941 gcggcgcagt tacgccgcct ttgtaggaat taatcgccgg atgcaaggtt cacgccgcat
     9001 ctggcaaaca tcctcactta cacatcccga taacacccca accgataacc acgctgagcg
     9061 atagcacctt tcaacgacgc tgatgtcaac acatccagct ccgttaaccg cgggaagcaa
     9121 taagcactct ggcgaatcgt attatcgata aacgccgggt ggcacatcac ttccagagag
     9181 cgctcgccgc gatgactgga gtcatcaagc acttgtagga acagtgtctc gctaatctct
     9241 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattagccgg taaatcacca
     9301 tcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacttt
     9361 gccacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
     9421 ggtttgcgtc caaataactc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
     9481 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
     9541 tcgcgagtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
     9601 cctatggcca gactcggttc atcaagactc aactgcaccg catggtcaat agcctgccca
     9661 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
     9721 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
     9781 agcctcggtt aatgtgcttt tttaagatct gcgatgcagt cggcaaaatt tggcaaccat
     9841 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
     9901 aacggactaa ggtttagggc cagtaacaca tcgttaaact ctccgctaag tgcggcgttg
     9961 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    10021 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca gcgtacaggt catttctacc
    10081 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    10141 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    10201 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atatagctca
    10261 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    10321 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    10381 aacagattca tagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    10441 ccagatgctt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgtgatttg
    10501 ccatttacca gcacatcctt aatgaacacc atatggttga ggccgaacag atcgatagat
    10561 aaatcatcgc tgtctttcag catcagaaca tcgcgaataa acatcttcat gccgatcgga
    10621 atattacaca cgccgataaa gcgtttaaat ccggtatgac gataaacggc ttcagtgacc
    10681 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacatagttc ttcgacatct
    10741 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacagacc gcccgcaccg
    10801 ttagtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcgcgcgcc
    10861 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    10921 cgatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    10981 agatcaaaaa taatatccag tttcgcttta ccaccttcga catccaccag ccataattcg
    11041 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    11101 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    11161 gaatactgat atctggcata tctgcccccc ggacataaat aatccagcaa caggacagat
    11221 atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct tacgatagct
    11281 cttaggcgta aaggatgtta atttcttaaa cgttttaata aacaagctcg ggctactata
    11341 accggcctca aacgcaatat ccgtcactga atagttggtc atttccagtt gttttttggc
    11401 aaaattaata cggatttcat taataatctg cattggcgtt ttaccatagt atcgttgagt
    11461 cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga
    11521 ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc actgcggtat
    11581 atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac
    11641 catctcaaca aactcttcca gccctgtttc gcggaaattc aatgaagaaa tcactgtttc
    11701 cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagcgacaa agcaataagg
    11761 caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt tcaatatacg
    11821 cgtggcacca aattcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc
    11881 gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac
    11941 cagagtaaat tcataatagt cgtgctgatg cagtccgctg atactctcag ttttattgta
    12001 gataaacaca tggaaatttt tgccattaaa caactgctgt tctcgggcag tggcaatttc
    12061 cagcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct
    12121 caattaattc agtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt
    12181 ggaccagcac cagactcact ttcatcttac cttcgcccgc atcgccttca atcagtttcg
    12241 tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg gtttttgctg
    12301 cggcaaaatc gccctgcttc gcctgtttca gtgccgcata cgccaggctg cgcgcttgtc
    12361 cggagttgat gataagcccc atcaccactt cttccagctc ttcaacttcc gtttgcgtat
    12421 cgggaatatt atcgagatcc atcatacatc gttcctctct attcttaccg gcacgattac
    12481 ccgtaccggc atcgattaaa atttcagggc gttagcgata tcttcttcgc tctcttcttt
    12541 atcaatcgcg ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaacgttgc
    12601 gataccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
    12661 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaataatgcc
    12721 catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
    12781 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
    12841 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
    12901 aaagatagcc aggatcagac ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
    12961 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
    13021 accatattgc tgataggtcg cgatattttc cagcgcccaa ggcgtcataa tgccgttgtc
    13081 cagtgcggtc agcgccagcg caccatgaat accgaagaac cagagcagtg gaacaaagat
    13141 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
    13201 aataatctga tggaagttgg tgccccaggt attcaacgtc caggcaataa tccccatcac
    13261 ggaaagaata ataaagccgg gaattaaagc cgagaaggaa cgcgatactg aagcaggtac
    13321 gctatcgggc aatttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
    13381 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
    13441 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
    13501 cccggccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
    13561 tgccattaaa gacattatcc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
    13621 accattaagt gtttcaatgg ttgaggcatc caggcgaata cctaaggaat aaaaaaacga
    13681 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
    13741 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
    13801 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
    13861 atacccttaa aaaccgcaat ttaaatattg cggtattgat ttatgaaata actctttgac
    13921 gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt
    13981 aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt cgaccggttt
    14041 gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac
    14101 gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac
    14161 cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag aggtagacat
    14221 gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat
    14281 tatctgtcag ccagacactc cgcaagcctt aacctgcttc catgctctgg gtaacttgcg
    14341 aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt
    14401 atttctttga cctgataaat tgtctctcct tgacctgcgg ttatgaccct cttcacattt
    14461 cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc
    14521 cggtttccgt tgtaactcaa gctttcaggc gaagtgatta cttcgcagcc tgtggatcag
    14581 tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg
    14641 cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac
    14701 cacgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg
    14761 cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac
    14821 gatcataagc cgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg
    14881 ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata
    14941 cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat
    15001 gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct
    15061 ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat
    15121 aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga
    15181 ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag
    15241 cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg
    15301 ccaggaaact ggcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca
    15361 ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac
    15421 cgttaatatc aagggcgcgg tattggccag cgaacaggcg ctgcgcgaag cgggtattga
    15481 attaagtgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag
    15541 cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac
    15601 cggattcttc actaaatatg gtgacggcgg tacggacatt aatccactgt atcgtcttaa
    15661 caaacgtcag ggtaaacagt tactggcggc attaggttgc ccggaacacc tttataagaa
    15721 agcgccaacg gccgatctgg aagatgatcg cccttctcta ccagatgaag tggcactcgg
    15781 cgtgacctat gacaatatcg acgactatct ggaagggaaa aacgtacctg aacaggtcgc
    15841 cagaacaata gagaactggt atctgaaaac tgaacataaa cgccgtccgc caattaccgt
    15901 tttcgatgat ttctggaaga agtaataatt tgcacatatt ggtttgtgcg aaaaagagta
    15961 ctttgttcac gccggatgcg gcgtgaacgc cttattcgcc ccacaaaact atgcaaattc
    16021 aatatattgc atgagtcgaa taggcctgac aggcgtagca cgtctgacgg tgtaaccttt
    16081 gtcatcaacc cgcctctttt ttaatcgctc cccgcctgtt acactggata gataaccagc
    16141 attcggagtc aactgtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg
    16201 caatttatga atatcccgaa catttacgtt cattccttaa tgacttaccc acccgaccag
    16261 gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc aaaagcatta
    16321 acattcgcag ccgcgtcctt tctcatttac gtaccccgga tgaagccgcc atgctacggc
    16381 aatcccgacg catcagctgg atatgtaccg ccggtgaaat cggcgctcta ctccttgaag
    16441 cgcgattaat caaagagcag cagccgctgt ttaataaacg cttgcgccgc aatcgccagc
    16501 tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt
    16561 tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat
    16621 tgcagaccat cgctgatgaa caaaaacttt gttatggtct gttgggactg gagccgttaa
    16681 gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta
    16741 aagagagcca tgacgatcat gcgctacgtt tgcgccagtc tctggaacgt ttacgagtag
    16801 tttgttggcc gtggaaaggt gcggtggcgc tgaaagaaca gcacccggaa atgactcaat
    16861 atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa
    16921 tgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc
    16981 tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt
    17041 gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc
    17101 gaaccagcac gcgccctggt cggtagtgag cagtttatcg cccaattgcc acgccccgtt
    17161 aatgacaaac actaccccgc cacgtgagcc aaaggtagta aacgtacgtt cggcaattct
    17221 gactttcgcc ttacagacat ccaggcgtgt catgatgttg aaatccatcg acatctgccc
    17281 tgccgtcagt ttcgctttta ccacctggtc cgccgcaaag gcaaaaggct gtaacggttt
    17341 taaggtatgg ttaaagcggt ctgtgctttc aaggaacatc tcgccgcctt ccagcaacgt
    17401 cactatcctt tccatacctg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat
    17461 gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc
    17521 accggcagca tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact
    17581 cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa
    17641 ctcgggtgat ttacgttagt ggcgatcagg ctttttttgc atctgtttga gaacaaaaaa
    17701 gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac cgacggtctt
    17761 acgttttttg gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac
    17821 gttctgtcag acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcaaga
    17881 tgttgtaaat cttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt
    17941 ccatttttgc gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt
    18001 catgcattgc gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca
    18061 tgatttcgcg gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt
    18121 cctgatgcgg accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg
    18181 aggtagtggt gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa
    18241 caaacagtgc agttaattta cgcatattct atatccttcc tttcagttat ttattacggc
    18301 tttcttaagt agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat
    18361 cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa
    18421 aaagaaaatt tgatggagag taatgagaga atattacaac acgatgattt tgcagagatt
    18481 atgaagaact ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta
    18541 ttaatttata agaaagtaac ttaatacccg cagaatgatt tctgcgggta agtattagct
    18601 tattttttcg agcattaatc ccgcgcgtaa tcccaacgct accaaaggat tagggaataa
    18661 cacatactct acatcatggg ttacggtaaa acgttcctcc ccgtcctgcg ccagcaatgt
    18721 tcctttctca aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg
    18781 cgagtggcga gtaatttgcg aaaccacccg ataacggagc ggcggcgttc tcacgatacc
    18841 gacactctca ccagatagca gcgcagcaat tgcgctggca gtgactgcaa actggcgaag
    18901 atcgttttgt ccaaagggca acgctttgcc gagttccagc gtacaggcca gcgcgccaaa
    18961 atgtctggcg ctgaaatggg taaacgtacc accaggctcc tgatggaaca ccagtgcctc
    19021 cagacccgcc gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg
    19081 taatacaccg aaccgcggat gcaaggagcc acgaattgcg gtatggagat caaggtgcca
    19141 gcgcacagat tctttgccct ggtcataaaa atcttccagg cactgctcca gttcgcgcgc
    19201 ccgacaggtt tccccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcgattcat
    19261 atcgctatgg caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag
    19321 taaccgccaa cgtaacggga tctcaccgtg agatatcgcg ccaagcaacg cgtccagcat
    19381 ttccacaggt gccgtctcat taccgtggat ccccgctgaa atcaccagcg cgccttgcgg
    19441 tggcgttaat ggcgtcagtt ccagcacgcc atcgcccagc cagcgccagc gaacgccgtt
    19501 gatttctcgc tcggtgataa ccgttttttt acccgttaag gtcagagcaa gaaaattatc
    19561 catttccccc tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca
    19621 atacatccag tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag
    19681 taaggcgatc gcggtagtaa cgatccaccc agtcattgag cgcattaaac agcgtatcgt
    19741 tcatcatcac cgccggattc actgcccggc gttcttcttc tgtcaatacc acgcgcaacc
    19801 gcaggcacgc cgggcctccg ccattcgcca tgctttcacg taaatcaaag acttttagtt
    19861 cgctaatcgg gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgtt
    19921 cccgacactc ctgaggcagc accaacatca tggaaccatc atcgcggctc agcagttggc
    19981 tgttaaacag atatgtagac accgcatctg acacggaaac ctgagtcgcc ggaacttcta
    20041 tcgccataaa accgttcacc cgcgcacgca ggcttgccag taactgtgac tggcgagcga
    20101 acgcctgttg atggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa
    20161 aaacgccctg gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca
    20221 cctgattcag ccttgccacc gcctcgctgg cttcgcgagt ctgccgcgcc ggataacggg
    20281 aaggccgggt atcattgcct tcttctcgcc cgtagacaaa aagttgcata cccggttcac
    20341 cgtaatgacc gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct
    20401 gcggcaacgc cgaatggaca ctaaatttct cttcgtcgtt aaaaatcgct tttaacaacg
    20461 attcggtgac gggggcttcc agcgaacggt gaaatttatt gttcaggttg gcaacggtga
    20521 gatgcacttt gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc
    20581 acattggcga agcggagctg acgctggaaa gccagtgcgg tgcctggcgg gcaacctttt
    20641 ccagtacctg ctcatcgctg ccgctgaatc ccaactgacg cagcaccgga ataaacggac
    20701 gctcgtgcgg cgggatcacc gcctggggga atcccgcgtc ggcaagggct ttcattttca
    20761 gtaagccctg cttcgccgcc agtcgcgggt tagagacctg aaaacggtga cgggtggagg
    20821 cttcattacc aaacgacagg cccgcgtaat gatgcgtcag ccctaccagc ccgtcgaaat
    20881 tgatttccca ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt
    20941 tgcgggcaat gttaacgagt ccgactccag acttgccatc ggccaggcac aataatctgc
    21001 ggcataccag gcgctggggc gatggttacc ggaagcgcca atgccgccga atggcgcggt
    21061 actggcagca ccggtaagcg gtttgttcca gttaacaatc cccgcccgtg cctccagcaa
    21121 cagttgatcg aatttttccc gctcggggga aaccagaccg caagagaggc cgaagcgagt
    21181 gttattcgcc attcgaatcg cttcatcgaa attgtcataa cgccagacgc gcaataacgg
    21241 cccaaacact tcttcatccg gcacgccagt aacgcctgtc atttcaatga tccccggcgt
    21301 cagcaacgat gtccctgctt gtaataagcg cggcgcaagc agggtccgtc cgcccatcgc
    21361 ctccagttcc tgccaggcag taaccacctg ctgtgcggcc tgctcagaaa tcagcccgcc
    21421 aataaacggc tgcggttcgt catcccaacg gcccggcgtt aaccgctggc tgacggcaac
    21481 caggcgagca agaaatgcat cgccctgcgt tccacttttc agcaataaac ggcgggcgca
    21541 ggtgcagcgt tgcccggcag tgacaaacgc cgactgaatg gtcagatgga cagcggcatc
    21601 gatatccgcc acctcatcga taattagcgg gttattaccg cccatctcaa gggcgagaat
    21661 tttctccggc tgaccggaaa gctggcgatg cagctggtag ccggtattgg cgctgccggt
    21721 aaacagcaag ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc
    21781 ctgcaccagg ttcagcacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc
    21841 ttcgccactc cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa
    21901 tgccggaacg atatgtccgt tcggtaaatg accggggaaa ttatacggcc caaacaccgc
    21961 cagcacgccg tgcgggcgat gtcgcaggct cgccgcgccg tccggcattt cactacgctg
    22021 ctcgccggta cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccat
    22081 cacttcggtt gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa
    22141 ttcggcttta ttgctttcca gcaatccggc aaagcgttca actcttacct gacgatcgcc
    22201 aaaggagaga cgcgcccagc gcggaaacgc cgcacgggca gcccgacaag cctgcccgac
    22261 ctgagcggca tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt
    22321 cacacgcgat gcgccctggc ccgtaaccca gtcaccattg atccataaag tcatgctgtt
    22381 ttctcctctg cgcacaggcg caccagacga acgcgatccc cggcgtggca tttcagggca
    22441 tccagttgtg cggcggttaa tatcaaacgc tcggttgccg gatcggtacg caccagcacc
    22501 acgcggaaat ggtgataatt ttcattggcg gccaggcagg ccgggaaatc gccctgcgca
    22561 ggctgccctt ctgccacttc caccagccga cttttgcgga tggcgcgcac gcggtcgatg
    22621 tcacactcaa gcgtcggccc gccgtcaaag atgtcgatat agttgcggta gcgaaaacct
    22681 tctttctcca gcaccgcgcg ggcgggcgcg gtttgcggat gtacctggcc gataacgtcc
    22741 tgcgcttcct gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttccgcaata
    22801 aatgcctttt gcccggtgcc acagagaaaa tcggcgcggc taaaatccat cgaaaagaag
    22861 cgtttaccga ggctttgcca gaacggcgaa tagccatgtt cgtcaatcac cccgcgcatt
    22921 tcggcgacca ctttgtcatt aaacttgtcg cgaaaggccg ccataaacat aaagcgcgat
    22981 ttcgacagca aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac
    23041 agctcgctgc tgccggtgtg atcgttactg agaaacagcg tcggcaacgc gttatagaca
    23101 ttcagctctt ttgaggcgtg aaccagcgtg ccgacgcgat agttgtacca gggatcgttc
    23161 agcccaaccg ccacctcaat ggcgcaaatc cccgccacag tgcctgtttc gctctcttcc
    23221 agtacaaaca cataaccctg ctcgcttttg ggcagttcgc cctgccaggt tttgatggcc
    23281 ctttcgatac gcgctgaaag cgtggcttca ttagcaggga gcgacgtcag gccgccgccc
    23341 gttttgctgg caagctgcat cagcgccgca acgtctgagc gctcaacggg acggatgacc
    23401 atcatgatga acctccgctc acaaagtgtt cacaagccgc cgcaaaacga tccagcccgg
    23461 tcgtcacctc ttcttcgctg acattgagcg ctggcgcaaa acgcactacg ttgccaccgg
    23521 caatcagcac cattacgccc gctttcgccg cttcctgaga aatttgtttc gcttgcccgg
    23581 cgtaatcggc attcagcaca cagccaatca gcagacctaa tccgcgaatt tcactgaaca
    23641 aaccgcagcg gtgattaatg gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa
    23701 cgccattaag catctctggc gtgttgatga gatccagcac tttacctgcc accgccgagg
    23761 ccagcggatt accgccataa gtggtaccat gtgtgcccac ggtcatcacg ctggcgcatt
    23821 cttcggttgc caacagagcg ccaacgggga aaccgccgcc cagtgctttg gcggtagtta
    23881 acagatcggg cgtcacgccg tagtgcatat aggcatacag ttccccggtg cgcccgacgc
    23941 cggtttgtac ttcatcaaaa atcaacagcg cattgtggcg gtcacacaat tcacgcagac
    24001 cttgtaaaaa tgcgttgctt gctggcacca caccgccttc cccctggatg ggttcgacaa
    24061 tcaccgcaca ggtagagtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcgg
    24121 catgacgaat atccggcggc agtggcgcaa aatcctgtga ataggctggc tgaccgcccg
    24181 cactgacggt aaacagcgtg cgaccatgaa acgcattttt aaacgccacg ataccgctct
    24241 tatggctgcc gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg
    24301 cttccgcacc ggagttacaa aagaagacgc gataggcaaa cgtggcgtcg atcaattttt
    24361 tcgccagtcg cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg
    24421 cctgttcgtt cagcgcctca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa
    24481 tgccacccgc gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc
    24541 cacgtaccgg tataaacggt gccggagcgt aaacaggtat catccattca tcaaagtttt
    24601 cacgcgtaat tggctgagac atagcgacct ctacagtaaa taattcgtta tttatatgtt
    24661 aataataagt aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata
    24721 aaagtgcata aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca
    24781 tttaaagtgc atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac
    24841 gcaatgttat gcagaagtaa aatataattc tggaattgtg atcattgacg aaatttactg
    24901 gaaattactg cgccattctg acgcaacgcg caccaaaagc gggcattttt tgcgccatcg
    24961 ttgacatcat taacaaccat cgatcaaatc acttaacaac aggcggtacg caaagcgaaa
    25021 ttctgctacc atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct
    25081 cttttaatat caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc
    25141 accaaccgga tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg
    25201 aagaggtggc gaagctcggc tacaacgtgt tttatcacgg acagaaaggc cattatggcg
    25261 tggcgctgct gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg
    25321 aagaggcgca acggcggatt attatggcgg aaatcccctc gccgctgggc aatgtcaccg
    25381 tgatcaacgg ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa
    25441 aggcgcagtt ttatcagaat ctgcaaaact atctggaaac cgaactcaag cgtgaaaatc
    25501 cggtgctgat tatgggcgat atgaatatca gtcctggcga cctggatatt ggcattggcg
    25561 aagagaaccg taagcgctgg ctgcgtaccg gaaaatgttc tttccttccg gaggaacgcg
    25621 aatggatgga gagactgatg agctggggat tggttgatac cttccgccat gcgaatccgc
    25681 aaacagccaa tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg
    25741 gtctgcgcat cgacctgctt ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg
    25801 gcatcgacta tgaaatccgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga
    25861 ccttccgccg ctaatttagc tgttctcctg gctcaaactg ggtcaggaga attaaccttg
    25921 agaaaaatca acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca
    25981 ctgcgttttt agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg
    26041 atgaacgctg agcgtaaatt tctttttgcc tgtcttattt ttgcgctggt catttacgct
    26101 ttccacgctt tcggtttatt cgagctgctc accgatttac ctcacttact gacactcatt
    26161 cgccagagcg gattgttcgg ttatagcctc tatattctgt tattcatcat tgccaccctc
    26221 tttctgttac caggaagcat actggtgatc gctggaggaa tagtttttgg cccgctctta
    26281 gggacactac tatcattaat tgccgccacg ctggcctcct cgtgctcatt cctgctggcg
    26341 cgctggctgg gacgtgattt actgctgaaa tacgttggcc atagccatac ctttcaggcc
    26401 attgaaaaag gcattgcgcg taacggtatt gattttctta ttctgacccg cttaatcccg
    26461 ttgtttcctt acaatattca aaattacgct tacggattaa ccacaatcgc cttctggcct
    26521 tataccctta tttcggcact cacaaccctg cccggtattg ttatttatac cgtgatggca
    26581 agcgatctcg ccaatgaagg cattacgctg cgctttattt tacaactctg tctggcgggc
    26641 ctggcactgt ttattctcgt ccagctcgca aaactctacg cccgacacaa acatgtggat
    26701 ctgtctgctt cgcgccgcag cccactttct caccccaaaa atgaaggata gaacgatgtt
    26761 gcaacattat tcagtgtcat ggaaaaaagg actggctgca ctctgcttac tggctgttgc
    26821 cgggcttagc ggctgcgatc aaaaagagaa tgcggctgca aaagtggaat acgacggact
    26881 ttcgaacagc caaccgctgc gtgtcgatgc caataaccat acggtaacca tgctggtgca
    26941 aattaatggt cgtttcctca ccgacgacac tcgtcacggt attgtgttta aagatggctc
    27001 caacggacat aaatcgctgt ttatgggtta tgcgaccccg aaagcctttt atgaagccct
    27061 gaaagaggca ggtggtacac cgggcgaaaa catgacgatg gataataaag aaacgactca
    27121 tgtcacaggc agcaaactgg atatttcggt caactggcaa ggggcggcaa aagcgtattc
    27181 cttcgatgaa gtgattgttg acagtaatgg aaagaaactg gacatgcgct ttggcggtaa
    27241 tttaacggca gcagaagaga agaaaacggg ctgcctggtg tgtctggata gctgcccggt
    27301 cggcatcgtc agcaatgcaa catacactta tggtgcggtt gaaaaacgag gtgaagttaa
    27361 attcaaaggc aatgcctcgg ttctcccggc ggataacacg ctggcaacgg ttacctttaa
    27421 aatcaccgaa taaagcagga taaaggatga tgaagatgca atcgcgaaaa atctggtact
    27481 accgtataac cctcatcatc ctgttgttcg ctatgctgct ggcatgggcg ctgcttcccg
    27541 gcgtccatga gtttatcaat cgcagcgttg cggcgtttgc cgccgtggac caacagggta
    27601 tagaacgctt tattcagtct tacggcgcac tggcggcagt tgtctcgttc ttgttgatga
    27661 ttttacaggc cattgctgca ccgctacctg cgtttttgat cacctttgcc aatgcgtcgc
    27721 tgtttggcgc gttctggggc ggcttgctgt cgtggaccag ttcgatggcc ggcgcggcgc
    27781 tgtgcttttt tatcgccaga gtgatgggcc gcgaagtggt ggaaaagtta acgggtaaaa
    27841 cggtactcaa tagtatggac ggctttttta cccgctatgg taaacacacc attctggtat
    27901 gccgattatt gccttttgtg ccttttgatc ccgtaagcta tgctgccggt ttgacttcaa
    27961 tacgttttcg ctcgtttttt atcgccaccg ggcttggtca gttaccagcg actattgttt
    28021 attcctgggc gggcagcatg ttaacaggcg gtactttctg gtttgtcacc ggactgttta
    28081 ttctgtttgc cctgaccgtg gtgattttta tggcgaagaa aatatggctt gagcgccaga
    28141 agaggaatgc ctgatgggtt taccgccgct tagcaaaatt cctttcattt tacgtccgca
    28201 ggcgtggctg catcgtcgcc attacggcga ggtgctaagc ccgattcgct ggtgggggcg
    28261 gatcccgttt atcttttatc tggtgtcgat atttgtcggc tggctggagc gcaaacgctc
    28321 accgctggat ccggtagtgc gatcgcttgt cagcgcgcgc attgcgcaaa tgtgcctgtg
    28381 tgagttttgc gtggatatca ccagtatgaa agtcgccgag cgcactggca gcaccgataa
    28441 actgctggca gtggctgact ggcggcaaag cccgctcttt agcgatgaag aacggctggc
    28501 gctggagtac gcagaagccg caagcgtaac gccgccaacg gtcgatgatg ccctgcgcgc
    28561 ccgactggtt acgcattttg acgctcaggc gctcaccgaa ctgacggcat tgatcggact
    28621 gcaaaacctg tcagcccgtt ttaattctgc catggacatt cccgctcagg ggctgtgccg
    28681 tattcctgaa aaacgttctt aaggagagat gatgcgccat tgtgtgtggt tgctgggatt
    28741 gttatcgctg ttttctttgg caacacatgc cagtgactgg caggaaatca aaaacgacgc
    28801 caaagggcaa accgtctggt ttaacgcctg gggcggcgat accgcaatta accgctatct
    28861 cgactgggtt agcggcgaga tgaaaaccca ttacgatata aacctgaaga ttgttcatct
    28921 cgcggatgcc gcagacgcgg tgaagcgcat tcagaccgaa gctgctgccg gacgcaaaac
    28981 aggcggttcg gtggatctgc tctgggtgaa cggcgaaaac ttccgcacct taaaagaggc
    29041 taatttactg caaaccgact gggcagagac tctgcccaac tggcgctatg tcgacacaca
    29101 gcttccggtg cgggaagatt tttcagtgcc tacagaaggg gctgaatcgc cctggggcgg
    29161 cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac aaacgccgca
    29221 agccttactg gagtttgcta aagccaatcc cggcacggtt acctatccgc gcccaccgga
    29281 ctttaccggc acggcgtttc ttgaacagtt gctgattatg ttgacacccg atcccgccgc
    29341 attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct tgtggcaatt
    29401 tcttgatgcg ctgcatccgt atttatggcg cgaaggaaag gatttcccgc cttcgcccgc
    29461 gcggatggat gctctgctga aagctaacac attgcgcctg tcgctgacct ttaatcctgc
    29521 tcatgcgcag caaaaaatcg ccagcggcga tttacccgcc agcagttaca gctttggctt
    29581 tagcgagggg atgattggca acgtccattt cgtcaccatt cccgccaacg cgaatgccag
    29641 tgctgcggcg aaggtagttg ccaatttcct gctctcaccc gatgcacaac tgcgtaaagc
    29701 agatcccgct gtctggggcg atccttctgt tctcgatccg caaaaactgc ctgacgggca
    29761 gcgcgaatca ttgcaatcga gaatgccgca ggatctgccg ccggtactgg ctgaaccgca
    29821 cgcaggttgg gtaaatgcgc tggaacaaga atggctacac cgttacggta cgcattaatc
    29881 tttttgctgt gggcgatgat ggcggtgatt tatgcaccgc tgatcccggc agttctcacg
    29941 ctgatatcgc ctgctttgtc gttgacgcac tggcaggcgt tatttgccga tccacagtta
    30001 ccgcaggcat tactggcaac gctggtgtcg acaaccattg cggcggtcgg ggcgttgttg
    30061 attgccctgc tggtgattgt agcgctgtgg ccagggccga aatggcagcg tatgtgcgcc
    30121 cgtctgccgt ggctgctcgc cattccccat gtggcttttg ccaccagcgc ccttctgctc
    30181 tttgctgacg gagggctgct ttatgactat ttcccgtatt tcactccgcc aatggacaaa
    30241 ttgggtattg ggctgggcct caccctggca gtgaaagaaa gcgcatttct gctgtggatc
    30301 ttagcggcag tattgagcga aaaacggctg ttgcagcaac tcattgtgct ggattcgctg
    30361 ggctacagcc gctggcaatg cctgaactgg ctgctgttgc cctctgtcgc gcctgcgctg
    30421 gcaatggcga tgctggcgat tgtggcctgg tcgctgtcgg tcgtggatgt ggcgattatc
    30481 cttgggccag gtaatccccc gacgctggca gtaattagct ggcagtggtt aactcagggc
    30541 gacgccgatc aacaagcgaa aggcgcactc gccagcctgc tgctgatgct gttactcgcc
    30601 gcctacgttt tgctgggcta tctgctatgg cgtagctgga ggcgtactat tccccgcgta
    30661 gatggcgttc gtaagcctgc cacgccttta ttgcctggca ttactctggc gagtttttta
    30721 cccttgaccg gtgtgctgtg tgtggttctg ttggcgatcc tcgcggatca gtcgacgatc
    30781 aatagtgaag cgctcatcaa tagcctgacg atgggactta ccgcgacgtt tatcgcttta
    30841 atcctgatac tggcgtggct ggaatgggga tcgtcacgtc ggcatttctg gctatggttg
    30901 cccattttat tacccgctct gccactggtg gcgggccagt acacgctggc gctatggcta
    30961 aacctagatg gaagctggac ggcggtggtc tgggggtatc tgctgtgggt aatgccgtgg
    31021 atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat tttgattgcg
    31081 caaacactgg gctggtcgcg gtccaaaatc ttcttttacg tgaaatgtcc gcttatgcta
    31141 cgccctacgc tgattgcctt cgcggtggga ttttcagtca gtattgcaca gtatatgccg
    31201 acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaagcggt ggcattaagc
    31261 agcggcggca gcaacggcat tctcgccgcc caggctttat ggcaactgct attaccgctt
    31321 attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag acaaggactc
    31381 cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc ttgctgacaa
    31441 acgttaactt tacggtggat aaaggtgaca ttgtcacgtt aatggggccg tctggctgtg
    31501 gaaaatccac tctgttttca tggatgattg gtggactggc cggacagttt tcttgtacag
    31561 gggagctatg gctcaatgag caacggattg acatgctacc caccgcacag cgtcagattg
    31621 gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa aatttactgc
    31681 tggcgctacc ggcgacactt aaagggaatg cccgacgtaa tgccgtgaat gatgcacttg
    31741 agcgttcagg ccttgacgga gctttccatc aggatcctgc tactttgtct ggcggtcagc
    31801 gagcgcgcgt tgctctgcta cgcgcccttc tcgcccagcc aaaagcgtta ctcctggatg
    31861 agccattcag ccgtctagat gtggctctgc gcgataattt tcgccagtgg gtgttcagcg
    31921 aagttcgcgc ccaggcgatc cccgtcgttc aggtaacgca cgatctccag gatgttcctg
    31981 ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa ctgcgataac
    32041 gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca atgtcgggat
    32101 tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg ctggcttgcg
    32161 cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt caacaacaaa
    32221 atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca caaaccttaa
    32281 atggcccttc tggtcatgaa cctgccgcct taaacctctc tgctaactgg ctcgacaaaa
    32341 tgagcaccga acagctcaac gagtggatca agcaacataa cctgaaagcc gatgctccgg
    32401 tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aaagcgactg caaaaagcag
    32461 gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt ctgcaaaaac
    32521 tgccgcattt tgagcagctg gtttatccgc aatggctgca cgacctgcaa caaggtaaag
    32581 aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg ggcgctccta
    32641 agctttacct tatcagccat attcccggcg ctgactacat cgataccaac gaagtggaaa
    32701 gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg gcaaaacacg
    32761 gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct gcagcgcgtg
    32821 tggcacaaat tatgctctac gctggcgtga aagatgtccg cctgctggac ggcggctggc
    32881 aaacctggtc cgacgcggga ctgcccgttg agcgcggaac gccaccgaaa gtgaaagcgg
    32941 aaccggattt cggcgtgaag atcccggcac aaccgcaact gatgcttgat attgaacaag
    33001 cgcgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg tggccagaat
    33061 ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata gccggagcac
    33121 gttggggaca cgctggtagc gactcgacgc atatggaaga tttccataac ccggatggca
    33181 ccatgcgtag cgccgatgat attaccgcta tgtggaaagc atggaatatc aagccagatc
    33241 agcaagtttc attctactgc ggcaccggct ggcgcgcgtc agaaaccttt atgtacgcac
    33301 gcgcaatggg ctggaataac gtctccgttt atgacggtgg ctggtacgaa tggagcagcg
    33361 atccaaaaaa tccggtagca accggtgaac gcggcccgga cagcagtaaa taacattgca
    33421 attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc gtaaaggtcg
    33481 tcatccagca caacgcacca aatacccacg caaaccacgg aaaacaggcc ggaaagaggc
    33541 aactcagaac aaacagcaaa atcgtctcgc tgccttcggt taagccgccc agataataaa
    33601 acgatttatg cgcataaccg ggattatcaa tctgatgttt cgccgccagc gcagcaaaag
    33661 cgagaaaact gctgcccgtg ccgataaacg caaacaacag ccagcctccg gccagcgcgt
    33721 tttgttctgg tgcggcgaga ataaaaccaa acggcaccag cgcgtaaaag agaaaatcga
    33781 gagaaatatc gagaaagccg cccgcgtcgg taagtcctct gcgccgcgcc agcgcaccgt
    33841 ccagaccatc aagcagcctg ttcaacaaaa tgacgattag cgccgccaga taccagccca
    33901 gcgccagaaa cggcagtgcc agcacgccga tggcaaatcc cactaatgtt aaaccatctg
    33961 gcgtgatgcc gggtttatcc agcaccttca cacactgatg caacaaaggt ttaattcggg
    34021 gatgaagatg gcggtctagc acaggcactc cttaaatata aagcctttcc gattgagcaa
    34081 cagtgcggat attatggcat ttttcgctta tctgcccgtg tgtaatttat gaaaatgatt
    34141 gaagttgttg ccgccatcat tgaacgtgat ggcaaaattt tactcgcgca acgccccgcc
    34201 cagagcgatc aggcgggatt atgggagttt gccggtggta aagtcgagcc ggatgaaagc
    34261 cagcggcagg cgctggtgcg tgagttaaac gaagaactgg gcatcgaagc aactgttggt
    34321 gactatgttg ccagccatca gcgagaagtt tcggggcgga ttatccatct tcatgcctgg
    34381 cacgtacccg acttccacgg gacgttacag gcacatgaac atcaggcgct ggtctggtgc
    34441 tcacctgaag aggcgctgca atatccgctg gcccctgctg acattccatt attagaggcg
    34501 tttatggctt tacgcgccgc cagaccagcg gattagtgct aaggtttttg tcatcctgtt
    34561 ggcattgcag cagtattcct tcggctttaa ttaccgcccc ttcagaataa ttttgatcct
    34621 gataaacgca gcactgagta cagggctgcg ctgactgccc gcctgaactg aacacttctg
    34681 gcggtacgtt tacctccacg tccggacgat aatgcgggtt agccagtgca attattggaa
    34741 atgctaatac tacggcgaac aatgctcgac tcacagggaa ctccttaacg ttattgtctc
    34801 tgctactgat aacggtagcc gggtggcaaa actttagcgt ctgagttatc gcatttggtt
    34861 atgagattac tctcgttatt aatttgcttt cctgggtcat ttttttcttg cttaccgtca
    34921 cattcttgat ggtatagtcg aaaactgcaa aagcacatga cataaacaac ataagcataa
    34981 tcgtattaat atataagggt tttatatcta tggatcagac atattctctg gagtcattcc
    35041 tcaaccatgt ccaaaagcgc gacccgaatc aaaccgagtt cgcgcaagcc gttcgtgaag
    35101 taatgaccac actctggcct tttcttgaac aaaatcccaa atatcgtcag atgtcattac
    35161 tggagcgtct ggttgaaccg gagcgcgtga tccaatttcg cgtagtatgg gttgatgatc
    35221 gcaaccaggt gcaggtcaac cgtgcatggc gtgtgcagtt cagctctgcc atcggcccgt
    35281 acaaaggcgg tatgcgcttc catccgtcag ttaacctctc cattctcaaa ttcctcggct
    35341 tcgaacaaac cttcaaaaat gccctgacta ctctaccgat gggcggtggt aaaggcggca
    35401 gcgatttcga cccgaaaggg aaaagcgaag gcgaagttat gcgtttttgc caggcgctga
    35461 tgactgaact gtatcgccac ctgggcgcgg atactgacgt tccggcaggt gatatcggcg
    35521 ttggtggtcg tgaagtcggc tttatggcgg ggatgatgaa aaagctctcc aacaataccg
    35581 cctgcgtctt caccggtaag ggcctttcat ttggcggcag tcttattcgc ccggaagcta
    35641 ccggctacgg tctggtttat ttcaccgaag caatgctaaa acgccacggt atgggttttg
    35701 aagggatgcg agtttccgtt tctggctccg gcaacgtcgc ccagtacgct atcgaaaaag
    35761 cgatggaatt tggtgctcgt gtgatcactg cgtcagactc cagcggcact gtagttgatg
    35821 aaagcggatt cacgaaagag aaactggcac gtcttatcga aatcaaagcc agccgcgatg
    35881 gtcgagtggc tgattacgcc aaagaatttg gtctggtcta tctcgaaggc caacaaccgt
    35941 ggtctgtacc ggttgatatc gccctgcctt gcgccaccca gaacgaactg gatgttgacg
    36001 ccgcgcatca gcttatcgcc aatggcgtta aagccgtcgc cgaaggggca aatatgccga
    36061 ccaccatcga agcgactgaa ctgttccagc aggcaggcgt actgtttgca ccgggtaaag
    36121 cggctaatgc tggcggtgta gcaacatcgg gcctggaaat ggcgcaaaac gctgcgcgcc
    36181 tgggctggaa agccgagaaa gttgacgcac gtttgcatca catcatgctg gatatccacc
    36241 atgcctgtgt tgagcatggt ggtgaaggtg agcaaaccaa ctacgtgcag ggggcgaaca
    36301 ttgccggttt tgtgaaggtt gcggatgcga tgttaagcca gggcgtgatt taagacaatt
    36361 gtgccggata cagcgcaaac gccttatccg gccataaaaa tcttcagtat cagatggttg
    36421 aaagaacctt tatattatca ctccattgcc cgcctcttct gatggcgggc tttttttcgg
    36481 cgctctcttg cgtggcgaat cctttttctt cccttctcca ccgccgggag ccattattcc
    36541 ccgaaactgc cttaccgacg tgcgctttgc ctgatcaatc aactggtaca atgtccccac
    36601 cagcggctgc ataaagtcct gatagcgaca ctgcttttcg ctgatttgcg tcagcaccga
    36661 ttcccagtgc gcggtcatgt ccggtcgcgt cgccatttct ggcagcgaat ggaatagcgc
    36721 ttttccggcg tcggtggagt ggatatagcg cccttttttg gtcaggaagc ctcgcttgaa
    36781 cagcagttca ataatcccgg cgcgagtcgc ttctgtcccc agaccatcgg tcgcacgaag
    36841 gatctttttc agatctttgt cctgcacaaa acgtgctatc ccggtcatcg ctgaaagcag
    36901 tgttgcatcg gtaaaatggc gcggcggctg ggtttgccgc tctaccactt cacctttttc
    36961 acacagcaac tcatcgcctt tcgccaccac aggcagcggc gtgccgtcgt tttcttcatc
    37021 gcgctctttg ctgcctaaca gtgtgcgcca gcctgcttca gcaagaaaac gggctttagc
    37081 gacaaatttg cctttggcaa tgtccagttc gataacacac ttgcggaaca ccgcatccgg
    37141 gcagaactgc atcaaatact gacgggcaat caggttatag accttcgctt cgttctccgt
    37201 caggttgatc gcagaactcc gtgcggtcgg gatgatggcg tggtgcgcat cgactttttt
    37261 gtcatcccaa cagcggttgc gtatatctgg atcgaccact ggctgcggca acagatctgg
    37321 tgcatgaacg ctgatagcat tcatcaccgc gtggcgtccg gcaaaatgtt cttctggcaa
    37381 atagcggcaa tcggaacgcg gataagtgat tagcttgtgc gtttcgtaca gcttctggca
    37441 gatatcaagc acgttctgcg cgctcagacc aaaacgttta gccgcttcaa tctgcaacgc
    37501 cgaaagcgaa aaaggcagcg gcgcggattc tgattcccgt ttatcgttat agctggtgac
    37561 aatagctggt tgaccactaa tgcggttaac cacatgctcc gccagtggac gatgtaataa
    37621 gcgcccttct tcatcctggt acggttcaca cgcttcgctc ggttgccaga tagcggtaaa
    37681 ccgctcatcg gcaggtgtca cgatatgtgc tttgacttca aagaagtctt tcgccacgaa
    37741 gttttcaatc tcttcgtcgc ggcgcaccac cagtccgagt acgggcgtct gcacgcgtcc
    37801 cacggaaagc acgccctgat aaccggcatt gcgaccgaga atggtatacg cacgggtcat
    37861 attaatgccg tacagccagt cagctcgcgc acgcgccagt gcagaaacgc acagcggtac
    37921 aaactcgcta ttggagcgaa gacgatcgat cgcccgctca accgcctgcg ggttcaggtc
    37981 gtttatcaaa caacgctgta cctgctggcg cttttccggt gccagttgca ggtagtccag
    38041 cacttcatcc accagcagtt gcccttcgcg atccgggtct ccggcgtgaa cgatttcgct
    38101 ggcttcatgc aggaagcgtt tgatgacatt aagttgttta gtcacggagg gtcggggctg
    38161 taattgccac ttttccggga caatcggcaa atccgcaaga ttccagcgcg catagcggct
    38221 gtcgtaggcg tctggctgcg cctgctcaag caggtgaccg atacaccagg tcaccacctg
    38281 accattaccg cactcgataa agccatcgcc tttccggtgc ggtttaggca ggacatcagc
    38341 aatggcgcgc gccagactcg gtttttcggc aataaacaac cgcattgaat taacggatct
    38401 caaccatggc acgaccgccg cgcgctggca ccagttcgcc aatcgccgtc agttcaatgc
    38461 caaactcggc ggctgtagct ttgacctcat tttctgcttc cggcatgacc gccagcagca
    38521 aaccgccaga agtttgcgga tcgcacagca gttcgcgcac ttcacgcggc atttcaccca
    38581 tcagatgacc gtagctggca aagttacgtt cggtgccgcc aggcactgcg cccaacttaa
    38641 tgtactcttc aacccccggc aatttcggga tcgcgtcata gtcgacgcgc gcctgcacac
    38701 ctgcgccctg acacatttcg cttaaatgcc ccagtaaacc aaaacccgta acgtcggtca
    38761 tcgcttttac gccttcgatg ttggcaaagg acgcgcctgc gatgttcatc cggcacatca
    38821 cttccgtcgc cagtccctga tgttctggtt tcagcagtga ttttttctca gccgtggtaa
    38881 gaacgccaat ccccagaggt ttcgtcagga acagtttgca tccggcttgt gcggtactgt
    38941 ttttcttcac ccgctcggta ggtacgatcc ccgttaccgc cagaccaaaa atcggctctg
    39001 gcgcatcgat ggagtgaccg ccagccagcg caatccccgc ctgacggcat gcatggcgtc
    39061 caccttcggt cacttcgcgg gcaatttccg gggaaagttt gttaatcggc cagccgagga
    39121 tcgcaatcgc cataatcggt ttgccgccca ttgcgaagat atcgctgatg gcgttagtcg
    39181 ccgcaatgcg gccaaaatcg aaaggattat caacgatcgg cataaagaag tcggtggtac
    39241 tgataacgct ggtgccattg cccagatcgt ataccgccgc atcgtcgcgg gtttcattac
    39301 ccacaagcaa attcggatca acaaacttcg cctgctcact gtgcaggatg gtttccaaca
    39361 cttttgggga aattttacag ccgcaaccag cgccgtggct gtattgggtc aaacgaatcg
    39421 agttctcgct catggacatc tcctgtcaat gcaatccggg tatggtaacc ctcattccat
    39481 gaagtgataa gtgagagtgt ctgaattcct gcgcctttgc tcacaatcca gacagtttcg
    39541 cgacaattat cagaaataag tcacaaacgg cgtcgggtcc gggacgttta tcgacgtaga
    39601 tgctttcaac tgcggcgtac cgaggtagag aaaaccgaca attttatcct gctcacggca
    39661 accgaatgct tcacgcacta ccggactttc agttaatgca ccactgcgcc agatgccgcc
    39721 aaacccctgg gcaactgctg ccatttgcat cgccatgacc gcgcatcccg cagacatttc
    39781 ctgttcccag cgcgggactt tatgattttc ttcgcatttc gccaccaccg ttatgatgag
    39841 cggtgcgcgg aacggcgcat tacgcgcttt gtcgatggct ttgttatcac taccggcagc
    39901 aatcgccccc tgctccagta cggcgctgaa acgctcgcgc ccttcccctt caatcacaaa
    39961 gaaatgccac ggttgcatgg acttatggtc cggcgcacgc atagccgcac gcaggatgtt
    40021 ttgcagttgt tcacccgttg gcgcaggttc agccaggcgg gaggcgctac ggcgattgat
    40081 caatagttcg agtgcatcca tttgattaac tcctgtcatg atatttattc acaaaattaa
    40141 cacgagagtg gattttgtta cagcccagtc cgcaattcct gctgacaagt gccggttggg
    40201 tcattacgat aaccacatct attgcgcctg aaacaggtgt gaccttaagt tgggagaata
    40261 catgcgaacc ctttggcgat ttattgccgg attttttaaa tggacgtggc gtctgctgaa
    40321 tttcgtccgt gaaatggtac ttaacctgtt ctttattttt ctcgtgctgg ttggtgtggg
    40381 gatctggatg caggtcagtg gtggtgattc gaaagaaacg gccagtcgtg gcgcactgct
    40441 gctggacatt tctggtgtga tcgtcgataa acccgacagt tctcagcggt ttagtaaatt
    40501 aagccgccag ctgcttggtg ccagttccga tcgtctgcag gaaaactcac tgtttgatat
    40561 cgtcaacacc attcgccagg cgaaggacga ccgcaatatc accggtattg tgatggatct
    40621 gaaaaacttc gcaggcggcg accagccgtc tatgcagtac atcggcaaag ctctgaaaga
    40681 gtttcgtgat agcgggaaac cggtttatgc cgttggcgag aactacagcc aggggcaata
    40741 ttatctcgcc agtttcgcca ataaaatttg gctgtctccg caaggcgtgg ttgatctgca
    40801 cggctttgcc accaacggtc tgtactacaa atcattactg gataagctga aagtttccac
    40861 ccatgtgttc cgcgtgggta cgtataaatc tgccgttgaa ccgtttattc gtgatgatat
    40921 gtcgccggca gcccgcgaag ctgacagccg ctggattggt gagctgtggc aaaactatct
    40981 gaatactgtt gccgctaacc ggcagatccc tgctcagcag gtattccctg gcgcgcaagg
    41041 gttgcttgag ggtttaacca aaaccggtgg cgataccgcg aaatatgcgc tggaaaacaa
    41101 gctggtcgat gcactggcat cgagtgccga aatcgaaaaa actctgacca aagagttcgg
    41161 ctggagcaag actgataaaa attatcgcgc catcagttat tacgattacg cattgaaaac
    41221 gccggcagat accggtgaca gcatcggagt cgtcttcgct aatggcgcaa ttatggatgg
    41281 cgaggaaact caggggaatg ttggcggtga taccactgcg gcacaaatcc gcgacgctcg
    41341 ccttgacccg aaagtgaaag cgattgtcct gcgtgttaat agcccaggcg gcagcgttac
    41401 cgcgtctgaa gtgattcgcg ctgaactggc agcagcccgg gcagcgggta agcctgtggt
    41461 tgtatcgatg ggcggcatgg cggcatctgg tggttactgg atttccacac cagctaatta
    41521 cattgtggct aaccccagca ccctgaccgg ttctatcggt atcttcggcg tgatcaccac
    41581 cgtagaaaat agtctggatt cgattggtgt tcatactgat ggtgtctcaa cttcaccgct
    41641 ggcggatgtt tctatcacca gggcactgcc gccggaagcg cagcagatga tgcagttaag
    41701 cattgagaat ggctataaac gctttatcac gctggttgct gatgcgcgtc attcaacgcc
    41761 agagcagatt gataaaatcg cccagggcca tgtctggacc ggtcaggatg caaaagctaa
    41821 cgggctggtc gatagtctcg gggatttcga tgatgcggtc gccaaagcag cagagctggc
    41881 aaaagtgaaa cagtggcatc tggaatacta cgttgatgaa ccgaccttct tcgacaaagt
    41941 gatggacaac atgtctggtt ctgtccgggc aatgttgcca gatgcgttcc aggccatgtt
    42001 acctgcaccg ctggcctcgg tagcctctac cgttaaaagt gaaagcgaca agctggcagc
    42061 gtttaacgac ccacaaaacc gttatgcgtt ttgcctgacc tgcgccaacg tgcgttaagt
    42121 cttgtactga gtggccgaca gatcgtcggc cacatttttt tacctcgacg aatcctcttc
    42181 ccgctgtctc gccccatatt tccttatccg catagtatca ggtgcgctcc cccttcctca
    42241 cgtatatact tttgctcttt cgatatcatt catatcaata tcatgcaaaa gaaatcaatt
    42301 tacgtcgcct acacgggcgg gaccatcggg atgcagcgtt ccgagcaggg ttatattccg
    42361 gtgtcaggtc atctacagcg ccaactggcg ctgatgccgg aattccatcg cccggagatg
    42421 ccagatttca ctattcatga atatacgcca ttgatggatt cttctgatat gacgccagaa
    42481 gactggcagc atattgctga agatattaaa gcgcactatg acgactatga tggttttgtc
    42541 attctgcacg gcacagacac gatggcgtat accgcctctg cactgtcgtt catgctcgag
    42601 aatctcggta aaccggtcat tgtgacaggg tcacaaatcc cgctggctga gttacgctct
    42661 gacggacaaa ttaatctgct gaatgcgttg tacgttgcgg cgaattatcc tatcaacgaa
    42721 gtaactctct tttttaataa tcgactgtat cgtggcaaca gaaccaccaa agcccatgcc
    42781 gatggttttg atgcgtttgc ctctccaaac cttcctccgt tactggaagc aggtatccat
    42841 attcgccgtt tgaatacgcc acccgccccg cacggtgaag gggaattgat cgttcatcca
    42901 atcacgccgc aaccaattgg cgtagtgacg atttatccgg ggatttctgc tgacgtcgtg
    42961 cgcaattttc tgcgccaacc tgtgaaagca ttgattctgc gctcatatgg cgtgggtaat
    43021 gcgccacaaa acaaagcctt cctgcaggaa ttacaagaag ccagcgatcg cggtattgtg
    43081 gtggtcaacc tgacacaatg tatgtccggt aaagtgaaca tgggtggtta tgccaccggt
    43141 aacgccctcg cccatgccgg cgttattggc ggtgcagata tgactgtaga agccacgcta
    43201 accaaactgc attacctgct tagccaggaa cttgatactg aaaccattcg caaggccatg
    43261 agccaaaacc tgcgtggcga actgacgccg gatgattaag gagactgcaa tgcaccctcg
    43321 cgccctgtta ctggtcgatt tacaaaatga tttctgtgct ggtggcgcgc tcgccgtgcc
    43381 ggaaggtgac agtacggtgg atgtcgctaa ccgcctgatt gactggtgcc agtcgcgcgg
    43441 tgaagcggtt atcgccagtc aggactggca cccggcgaat cacggcagtt ttgccagtca
    43501 gcacggtgta gagccttata cgccaggcca actcgacggt ttgccacaaa ccttctggcc
    43561 agatcactgt gtgcagaaca gtgaaggcgc acaattacat ccgttactga accaaaaagc
    43621 gatcgcagcg gtgttccata aaggcgaaaa tcctttagtt gacagttaca gtgccttttt
    43681 tgataacggc cgtcggcaga aaaccacgct cgatgactgg ttacgcgatc atgaaatcga
    43741 tgaattgatc attatgggtc tggctactga ctattgcgtg aagtttaccg tgctggacgc
    43801 gttacagtta ggttataagg ttaatgtgat taccgatggt tgtcgtggcg tgaatatcca
    43861 gccacaggac agtgcgcacg cgtttatgga gatgtcagca gctggggcaa cgctatatac
    43921 gctggcagat tgggaagaga cgcaggagta atattagttg gattcatctc tggcctacat
    43981 ttcctcaatg acattgtagg ccaggtaata cttgttagtg ttgcatttaa cctactcgca
    44041 gctaaaacac gacatccgaa caagtctatt tgccctccct taataacagg cttaaatact
    44101 agacaactca ttcacttaaa catggcaata ggctttgctg agataccaaa atcttctttt
    44161 aactgttgtt tgctcttcat gaccatctga ccatttgtgt caattgtcat atgctgtgct
    44221 tcggtattat tgcgggcttg ccacagcatc accagttgca ggcagttctc tttttgttcc
    44281 tcggtcaacg taacgccatc aggccatttc cccagttcaa cggcggtcga caaacgctgg
    44341 tatacctcag gcgtcatgct gttgataatg tcatcaagat tcataatttt tccgctcctg
    44401 tggaataagt tgctgaatcg ttttttcaac cgttgatttc ttcgccgttt tcgccatcag
    44461 tgaagcgtaa agaagcagag ttaacacaat aacgttcgcc cgttggctgc ggcccgtcag
    44521 ggaagacatg gcccagatgg gcatcgcagt taccgcaacg aatttctatg cgctgcattc
    44581 catgtgacaa gtctttgata taacgaatag attcttcact caccggttcg tagaaactgg
    44641 gccagccaca gccggaatca tacttggttt gggaatgaaa cagcggggca tcgcagatca
    44701 aacagtgata tacgccgtca cgcttgttat gcagtaaacg acccgtaaat ggcggttctg
    44761 tcccatgatt ctgcgtcacg tagaactgca tctcggacaa attttttttc agttcttctg
    44821 ccgaaggttt attagccatt tgctcacatc tcactttaat cgtgctcaca ttacgtgact
    44881 gattctaaca aaacattaac accaactggc aaaattttgt cctaaacttg atctcgacga
    44941 aatggctgca cctaaatcgt gatgaaaatc acatttttat cgtaattgcc ctttaaaatt
    45001 cggggcgccg accccatgtg gtctcaagcc caaaggaaga gtgaggcgag tcagtcgcgt
    45061 aatgcttagg cacaggattg atttgtcgca atgattgaca cgattccgct tgacgctgcg
    45121 taaggttttt gtaattttac aggcaacctt ttattcacta acaaatagct ggtggaatat
    45181 atgactatca aagtaggtat caacggtttt ggccgtatcg gtcgcattgt tttccgtgct
    45241 gctcagaaac gttctgacat cgagatcgtt gcaatcaacg acctgttaga cgctgattac
    45301 atggcataca tgctgaaata tgactccact cacggccgtt tcgacggtac cgttgaagtg
    45361 aaagacggtc atctgatcgt taacggtaaa aaaatccgtg ttaccgctga acgtgatccg
    45421 gctaacctga aatgggacga agttggtgtt gacgttgtcg ctgaagcaac tggtctgttc
    45481 ctgactgacg aaactgctcg taaacacatc accgctggcg cgaagaaagt ggttatgact
    45541 ggtccgtcta aagacaacac tccgatgttc gttaaaggcg ctaacttcga caaatatgct
    45601 ggccaggaca tcgtttccaa cgcttcctgc accaccaact gcctggctcc gctggctaaa
    45661 gttatcaacg ataacttcgg catcatcgaa ggtctgatga ccaccgttca cgctactacc
    45721 gctactcaga aaaccgttga tggcccgtct cacaaagact ggcgcggcgg ccgcggtgct
    45781 tcccagaaca tcatcccgtc ctctaccggt gctgctaaag ctgtaggtaa agtactgcca
    45841 gaactgaatg gcaaactgac tggtatggcg ttccgcgttc cgaccccgaa cgtatccgta
    45901 gttgacctga ccgttcgtct ggaaaaagct gcaacttacg agcagatcaa agctgccgtt
    45961 aaagctgctg ctgaaggcga aatgaaaggc gttctgggct acaccgaaga tgacgtagta
    46021 tctaccgatt tcaacggcga agtttgcact tccgtgttcg atgctaaagc tggtatcgct
    46081 ctgaacgaca acttcgtgaa actggtatcc tggtacgaca acgaaaccgg ttactccaac
    46141 aaagttctgg acctgatcgc tcacatctcc aaataagttg agatgacact gtgatctaaa
    46201 aagagcgact tcggtcgctc ttttttttac ctgataaaat gaagttaaag gactgcgtca
    46261 tgattaagaa aatttttgcc cttccggtca tcgaacaaat ctcccctgtc ctctcccgtc
    46321 gtaaactgga tgaactggac ctcattgtgg tcgatcatcc ccaggtaaaa gcctcttttg
    46381 cattacaggg cgcacacctt ctctcatgga aacctgcggg tgaagaagaa gttctgtggt
    46441 tgagcaataa cacaccgttc aaaaatggcg tcgctattcg cggtggcgta ccggtttgct
    46501 ggccgtggtt tggtccggct gcacaacaag gtccggctgc acaacaaggt ctgcctgcgc
    46561 acggttttgc ccgcaacctg ccgtggacgc tgaaatcaca tcgtgaagat gctgatggcg
    46621 tagcgctgac ttttgaattg acacaaagcg aagagacgaa aaaattctgg ccgcacgact
    46681 ttacgctatt agcgcatttc cgcgtgggta aagcctgtga aatcgatctt gaagcacacg
    46741 gcgaatttga aaccacctct gccctgcata cctactttaa cgtgggtgat atcactaagg
    46801 taagcgtcag tggactgggc gatcgcttca ttgataaagt gaatgacgcg aaagaagatg
    46861 tactgaccga cggtattcag accttccctg accgtaccga tcgcgtgtat ctgaatccgc
    46921 aagattgcag cgtgattaat gacgaagcgc tgaatcgtat tatcgccgta ggccaccagc
    46981 atcatctgaa cgtcgttggc tggaacccag gaccggcgct ttcagttagc atgggcgata
    47041 tgccggacga tggctacaaa acctttgttt gtgtagaaac ggcttacgct tcagaaacgc
    47101 aaaaagtgac caaagaaaaa cctgcacatc tggcgcaatc cattcgcgtt gcgaaacgtt
    47161 aatttgcgtt aatgttgtgt gccgggtgcg ttgcatccgg cacgtatcaa catcacacca
    47221 tatccagcgc agtttttcct tttggtgccg gatatgcctt atccagcata gctaattccg
    47281 ctgacgaaag ttcaacctca agcacagccg cattttgttg gacatgggca atcgtggccg
    47341 cttttggaat cgccatcaca ccctgatgac tgatcaccca cgccaacaat acttgtgccg
    47401 cgctgatatt gtgagcatgt gcaatttcgt tgactactgc gttttttagc agcccattgc
    47461 gcaaccgccc ggcctgagct aacggactgt aagccatcac cggtatctgc tgttgctggc
    47521 accaggggag tagatcgtac tcaatccctc gtgaaccgag atggtaaagc acctgattag
    47581 tggcacactg atttcccccc ggcagctgcc agagttcctg catgtcggca taatcaaggt
    47641 tagaaacgcc ccagcggcgg atttttccct gggcgatcaa tttttccatc gctgcgacag
    47701 tctcttcaaa agcgaaactg ccagaccagt gtaataagta gagatcgaga taatcagtat
    47761 tgagacggcg taaactggct tcgcatgcat ttatcgcttt ttgcccgcca gcattccacg
    47821 gatagacttt agagacgaga aagacgttat cacgcagacc ggttaatgct tccccaacca
    47881 ccttttcagc accgccttcg gcatacattt cggcggtatc aatgagggtt aaaccgagtt
    47941 caatgcccgc gcgtagtgca gcaacttctg ttttgcgctg actggcatct tcgcccatat
    48001 accatgttcc ctgccctatg gctggcagtg agacatcgcc actaaattga atcatttttt
    48061 gttgcattgt ttcctcccag gtattgcacc accgtaatgc aaaacagggc atgacgccct
    48121 gtttttatgc acaaaatgcc ctggaaagat gcattatcag aatttgtagg tgatcccggt
    48181 agaaatcagg ccagtccagg atttatccac cattgggctg tcagtaactt catcagacag
    48241 acgggtgtag cgcgcggtac cgtaaacact ccagtcgccg aggaagttgt agctggcgct
    48301 cagctccagg taagggctcc agctgtcgtt cgggttatag ccacgcagac cgctgcgagc
    48361 ggactctttg cgcgatacgc cataatagta ttcgttctgg ttttcgctgt tccactgcac
    48421 accaataccc ggagtcacgg tcaggccacc gttggtgtaa cgatacaacc aggccatatc
    48481 ccagacgatg ccgttgctgt tatccagggt atcgccagcc agggtggtac gcaggtaacc
    48541 gtactgggta aagtgagcat aagacagacc agccatcatg gtgctcttac ggtcatccag
    48601 gtgacgcatt tggtgatcgc cactgtcttt agctttgaag taaagcggcg accagtaagc
    48661 ggtaattgaa agtttgtccg ttgcgtcatt ccacaggtag taaccaccac ctaagccacg
    48721 gaaccagaag ttatcgccgt catagttgat taccggtact gggtaaacat cggtatcgta
    48781 atctttatat gggtgttcaa cgacacctac gcctgcgccc agggaaaatt taccttcagc
    48841 gtgcgctacg cctgcagacg ttgcgataag cactccaagt gccagaagtt tgagtttggt
    48901 cacaattaat cattccttaa acaaatgttt agcgggcgac aaagtttacc cgtcaataca
    48961 tcgaaaccca acctttttac gttttcattt tttatagtaa ctgtttaatt ttcctgacgc
    49021 agatgacacc actcttacag ccaaatgaat ttagcgttac cggcgaccct ggtatttaca
    49081 ttaattatgc aaaatttatg gatgagttgt tgatatgcca ttgaaattaa gaaagccgtg
    49141 caggcaagtt ttccatttgc catctacgct taattttgaa ggtgtatcac cgggcacgtt
    49201 gttctcatcg tcgataaaat ggcatgagag ttgctgtgtt ttagcaagag acgtcgttca
    49261 gtttacttct tccgggagcc tctactattc atatgaacgg ctcttaacct gtgctaaaaa
    49321 acgaaaggac ggcataccat gaatatattc gatcactatc gccagcgata tgaagctgcc
    49381 aaggacgaag agttcacact gcaggagttt cttaccactt gtcggcaaga tcgcagtgct
    49441 tatgccaacg cggctgagcg gctattgatg gctatcggtg agcctgtcat ggtcgataca
    49501 gcccaggaac ccagactttc tcgactcttt tctaaccggg tcatagcacg ttatccggca
    49561 tttgaagagt tttacggcat ggaagacgcg attgaacaga ttgtctctta tctgaaacac
    49621 gcggctcagg ggctggaaga gaagaaacaa atcctgtatc tgcttgggcc tgtgggtggg
    49681 ggtaaatcat cgcttgctga gcgactgaaa tcattaatgc agctcgtacc gatttatgta
    49741 ttgagcgcca acggtgagcg tagcccggtc aacgatcatc cgttctgtct tttcaatccg
    49801 caggaagatg cgcagattct ggaaaaagag tatggcattc ctcgccgtta tctcggcacc
    49861 atcatgtcgc cgtgggcggc aaaacgcctg catgaatttg gtggcgatat cactaagttc
    49921 cgggtagtga aggtctggcc gtcaattctg caacaaattg ctatcgccaa aacggaaccc
    49981 ggtgatgaga acaaccagga catctccgcg ctggttggga aagtcgatat tcgtaaactc
    50041 gaacactacg cgcagaatga cccggacgcc tacggctatt ccggtgcact gtgccgcgcc
    50101 aaccagggga tcatggaatt cgttgagatg tttaaagcac cgattaaagt gctgcatccc
    50161 ttgctaaccg ccactcagga aggtaactac aacgggacgg aaggtatctc cgccctgccg
    50221 ttcaacggga ttattctcgc ccactcgaac gaatccgaat gggtcacttt ccgtaataac
    50281 aaaaacaacg aagccttcct cgatcgtgtt tacatcgtga aggtgccgta ttgcttgcgc
    50341 atttccgaag agatcaaaat ctacgagaaa ttgcttaatc acagtgaatt gactcacgcc
    50401 ccctgcgccc ctggcacgct ggaaacgctg tcacgttttt ccattctttc gcgccttaaa
    50461 gagccagaaa actccagcat ttattcaaag atgcgggttt atgatggcga aagtctgaaa
    50521 gacacagatc ccaaagccaa gtcgtatcag gaatatcgtg actacgccgg tgtcgatgaa
    50581 gggatgaacg gtctgtcgac gcgttttgcg tttaagatcc tctcccgcgt gttcaacttc
    50641 gatcatgtag aagtggcggc aaacccggtt catctgttct acgtcctgga acagcagatc
    50701 gagcgcgagc agttcccgca agaacaggca gaacgctatc tggagttcct gaaaggttat
    50761 ctgatcccga aatatgccga gtttatcggc aaagagatcc agacggccta ccttgaatcc
    50821 tattccgaat atgggcaaaa cattttcgac cgttatgtta cctacgcgga tttctggatt
    50881 caggatcagg agtatcgcga tcctgatacc gggcagttgt ttgaccgcga gtctcttaac
    50941 gccgagctgg agaaaatcga gaaaccggcg gggatcagta atccaaaaga tttccgcaac
    51001 gagattgtta acttcgtact gcgcgccaga gcgaataaca gcggacgcaa tccgaactgg
    51061 accagctatg aaaaactgcg cacggttatt gagaagaaaa tgttctccaa taccgaggag
    51121 ctgttgccgg ttatttcgtt taacgccaaa acgtcaaccg acgagcaaaa gaaacatgac
    51181 gactttgtcg accgtatgat ggaaaaaggc tacacccgta aacaggtgcg tttactgtgc
    51241 gaatggtatt tgcgcgtacg taaatcgtct taacaaccct ggcccggtac acatgctacc
    51301 gggcctacaa cgacagcgaa ccgtgggcca gagaagcggc aacacaggcg tagcataaag
    51361 ttggcaaatg tagtacgggg ggcatatgac ctggtttatt gaccggcgtc tgaacggcaa
    51421 aaacaaaagc atggtgaatc gccagcgttt tttacgccgt tataaagcgc aaattaaaca
    51481 gtcgatctcc gaggccatta ataagcgttc ggtgactgac gtcgacagcg gcgagtccgt
    51541 atccattccc acggaagata ttagcgaacc gatgtttcat caggggcgtg gcggtctgcg
    51601 ccaccgcgtg catccgggca atgaccattt cgtccagaac gaccgaattg aacgtcccca
    51661 gggtggcggc ggaggttccg gcagcggtca gggccaggcc agccaggatg gtgaaggtca
    51721 ggatgaattc gtctttcaga tttcgaaaga tgagtatctt gatctgctct ttgaagattt
    51781 ggctttaccg aatctgaaac aaaaccaaca acgccagctg accgaatata aaacgcatcg
    51841 ggcgggttat accgctaacg gcgttccggc caatatcagc gttgtgcgtt cattgcagaa
    51901 ctcactggcg cggcgcacag ccatgacggc gggcaagcgg cgggaacttc atgcgctgga
    51961 agagaatttg gccatcatca gcaacagtga acctgcgcaa ctgctggaag aggaacgtct
    52021 gcgcaaagaa attgcagaat tacgtgccaa aattgaacgc gtccctttca ttgacacctt
    52081 cgatttacgt tacaagaact acgaaaaacg gcccgatccc tccagccagg cagtgatgtt
    52141 ttgcctgatg gacgtttccg gttcaatgga tcagtccact aaagatatgg ctaaacgttt
    52201 ttatattctg ctgtatctgt tcctcagcag aacatataag aacgtggaag tcgtatacat
    52261 ccgccatcat acccaggcga aagaagtcga tgaacatgag tttttctact cgcaggaaac
    52321 aggcggcacc attgtttcca gcgccctgaa actgatggat gaggtagtga aagagcgtta
    52381 taacccggca cagtggaata tttacgcagc ccaagcatca gacggcgata actgggccga
    52441 tgactctccg ctttgccatg aaattctggc gaaaaaaata ttacccgttg ttcgttatta
    52501 cagctatatc gaaattacac gtcgcgcgca tcagacactg tggcgagaat atgagcatct
    52561 gcaatctact ttcgacaact ttgcgatgca gcatatccgc gaccaggatg atatttatcc
    52621 ggtgttccgt gaactgtttc ataaacaaaa tgcaacagct aaagactaaa actgtcagcc
    52681 gggtcattat cgcctggctg atttttagtt cactgtaaat tatctcattt attacataca
    52741 ttagcttaca atcgctttaa atatgacagc ataaccttta cacaatttag ttccagaaaa
    52801 caatcattcg gaaaaat
//
LOCUS       DAZKJR010000014        51499 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKJR010000014 DAZKJR010000000
VERSION     DAZKJR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.014"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(229..1629)
                     /gene="asnS"
                     /locus_tag="ACHOWS_000860"
     CDS             complement(229..1629)
                     /gene="asnS"
                     /locus_tag="ACHOWS_000860"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0046488.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1798..3000)
                     /gene="pncB"
                     /locus_tag="ACHOWS_000861"
     CDS             complement(1798..3000)
                     /gene="pncB"
                     /locus_tag="ACHOWS_000861"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0046489.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQYLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3266..5878
                     /gene="pepN"
                     /locus_tag="ACHOWS_000862"
     CDS             3266..5878
                     /gene="pepN"
                     /locus_tag="ACHOWS_000862"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFQ0046490.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVRWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQLLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6084..6851)
                     /gene="ssuB"
                     /locus_tag="ACHOWS_000863"
     CDS             complement(6084..6851)
                     /gene="ssuB"
                     /locus_tag="ACHOWS_000863"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFQ0046491.1"
                     /translation="MNTARLNQGTPLLLNAVSKQYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(6848..7639)
                     /gene="ssuC"
                     /locus_tag="ACHOWS_000864"
     CDS             complement(6848..7639)
                     /gene="ssuC"
                     /locus_tag="ACHOWS_000864"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFQ0046492.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7651..8796)
                     /gene="ssuD"
                     /locus_tag="ACHOWS_000865"
     CDS             complement(7651..8796)
                     /gene="ssuD"
                     /locus_tag="ACHOWS_000865"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFQ0046493.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8793..9752)
                     /gene="ssuA"
                     /locus_tag="ACHOWS_000866"
     CDS             complement(8793..9752)
                     /gene="ssuA"
                     /locus_tag="ACHOWS_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFQ0046494.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9745..10320)
                     /gene="ssuE"
                     /locus_tag="ACHOWS_000867"
     CDS             complement(9745..10320)
                     /gene="ssuE"
                     /locus_tag="ACHOWS_000867"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0046495.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10557..11567
                     /gene="pyrD"
                     /locus_tag="ACHOWS_000868"
     CDS             10557..11567
                     /gene="pyrD"
                     /locus_tag="ACHOWS_000868"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFQ0046496.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11741..12283
                     /gene="zapC"
                     /locus_tag="ACHOWS_000869"
     CDS             11741..12283
                     /gene="zapC"
                     /locus_tag="ACHOWS_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFQ0046497.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFNLEQAV"
     gene            complement(12280..13389)
                     /gene="ycbX"
                     /locus_tag="ACHOWS_000870"
     CDS             complement(12280..13389)
                     /gene="ycbX"
                     /locus_tag="ACHOWS_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFQ0046498.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEVLATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            13633..15741
                     /gene="rlmKL"
                     /locus_tag="ACHOWS_000871"
     CDS             13633..15741
                     /gene="rlmKL"
                     /locus_tag="ACHOWS_000871"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFQ0046499.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15753..17660
                     /gene="uup"
                     /locus_tag="ACHOWS_000872"
     CDS             15753..17660
                     /gene="uup"
                     /locus_tag="ACHOWS_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0046500.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17790..19043
                     /gene="pqiA"
                     /locus_tag="ACHOWS_000873"
     CDS             17790..19043
                     /gene="pqiA"
                     /locus_tag="ACHOWS_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFQ0046501.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADDPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            19048..20688
                     /gene="pqiB"
                     /locus_tag="ACHOWS_000874"
     CDS             19048..20688
                     /gene="pqiB"
                     /locus_tag="ACHOWS_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFQ0046502.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            20685..21248
                     /gene="pqiC"
                     /locus_tag="ACHOWS_000875"
     CDS             20685..21248
                     /gene="pqiC"
                     /locus_tag="ACHOWS_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFQ0046503.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            21504..21671
                     /gene="rmf"
                     /locus_tag="ACHOWS_000876"
     CDS             21504..21671
                     /gene="rmf"
                     /locus_tag="ACHOWS_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFQ0046504.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21741..22259)
                     /gene="fabA"
                     /locus_tag="ACHOWS_000877"
     CDS             complement(21741..22259)
                     /gene="fabA"
                     /locus_tag="ACHOWS_000877"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFQ0046505.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(22328..24088)
                     /gene="ycbZ"
                     /locus_tag="ACHOWS_000878"
     CDS             complement(22328..24088)
                     /gene="ycbZ"
                     /locus_tag="ACHOWS_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0046506.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            24274..24726
                     /gene="matP"
                     /locus_tag="ACHOWS_000879"
     CDS             24274..24726
                     /gene="matP"
                     /locus_tag="ACHOWS_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFQ0046507.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24802..25854)
                     /gene="ompA"
                     /locus_tag="ACHOWS_000880"
     CDS             complement(24802..25854)
                     /gene="ompA"
                     /locus_tag="ACHOWS_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFQ0046508.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVPGGASTKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(26210..26719)
                     /gene="sulA"
                     /locus_tag="ACHOWS_000881"
     CDS             complement(26210..26719)
                     /gene="sulA"
                     /locus_tag="ACHOWS_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFQ0046509.1"
                     /translation="MYTSGYAHRASSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            26937..27566
                     /gene="sxy"
                     /locus_tag="ACHOWS_000882"
     CDS             26937..27566
                     /gene="sxy"
                     /locus_tag="ACHOWS_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFQ0046510.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(27529..29691)
                     /gene="yccS"
                     /locus_tag="ACHOWS_000883"
     CDS             complement(27529..29691)
                     /gene="yccS"
                     /locus_tag="ACHOWS_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFQ0046511.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(29701..30147)
                     /gene="yccF"
                     /locus_tag="ACHOWS_000884"
     CDS             complement(29701..30147)
                     /gene="yccF"
                     /locus_tag="ACHOWS_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0046512.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            30270..32324
                     /gene="helD"
                     /locus_tag="ACHOWS_000885"
     CDS             30270..32324
                     /gene="helD"
                     /locus_tag="ACHOWS_000885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFQ0046513.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWQRWSSEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEVIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPSVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(32356..32814)
                     /gene="mgsA"
                     /locus_tag="ACHOWS_000886"
     CDS             complement(32356..32814)
                     /gene="mgsA"
                     /locus_tag="ACHOWS_000886"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0046514.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(32910..33572)
                     /gene="yccT"
                     /locus_tag="ACHOWS_000887"
     CDS             complement(32910..33572)
                     /gene="yccT"
                     /locus_tag="ACHOWS_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFQ0046515.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            33745..34158
                     /gene="yccU"
                     /locus_tag="ACHOWS_000888"
     CDS             33745..34158
                     /gene="yccU"
                     /locus_tag="ACHOWS_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0046516.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(34203..34520)
                     /gene="hspQ"
                     /locus_tag="ACHOWS_000889"
     CDS             complement(34203..34520)
                     /gene="hspQ"
                     /locus_tag="ACHOWS_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFQ0046517.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(34578..35768)
                     /gene="rlmI"
                     /locus_tag="ACHOWS_000890"
     CDS             complement(34578..35768)
                     /gene="rlmI"
                     /locus_tag="ACHOWS_000890"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFQ0046518.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVNIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            35863..36141
                     /gene="yccX"
                     /locus_tag="ACHOWS_000891"
     CDS             35863..36141
                     /gene="yccX"
                     /locus_tag="ACHOWS_000891"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0046519.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(36138..36467)
                     /gene="tusE"
                     /locus_tag="ACHOWS_000892"
     CDS             complement(36138..36467)
                     /gene="tusE"
                     /locus_tag="ACHOWS_000892"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFQ0046520.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRNFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(36558..37217)
                     /gene="yccA"
                     /locus_tag="ACHOWS_000893"
     CDS             complement(36558..37217)
                     /gene="yccA"
                     /locus_tag="ACHOWS_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFQ0046521.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(37627..38646)
                     /locus_tag="ACHOWS_000894"
     CDS             complement(37627..38646)
                     /locus_tag="ACHOWS_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0046522.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(38624..38866)
                     /locus_tag="ACHOWS_000895"
     CDS             complement(38624..38866)
                     /locus_tag="ACHOWS_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFQ0046523.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(38934..41372)
                     /locus_tag="ACHOWS_000896"
     CDS             complement(38934..41372)
                     /locus_tag="ACHOWS_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFQ0046524.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRFGIKGGFYWSTEKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQEKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINDGLA
                     AECKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQLETEQND
                     QKAEQNSQNALQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCGAVMGDATYQET
                     FDEKNQAEVQEDDPEEIEGAEHPNNENAGNDQHHTSDSETGEAADPLIAVNGHHEITS
                     TSRTCDHLMIDLETMGKNPNAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILR
                     RSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYV
                     SAIWQKLIPSQADF"
     gene            complement(41465..41653)
                     /locus_tag="ACHOWS_000897"
     CDS             complement(41465..41653)
                     /locus_tag="ACHOWS_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFQ0046525.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(41650..41838)
                     /gene="dicB"
                     /locus_tag="ACHOWS_000898"
     CDS             complement(41650..41838)
                     /gene="dicB"
                     /locus_tag="ACHOWS_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFQ0046526.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(42324..42476)
                     /locus_tag="ACHOWS_000899"
     CDS             complement(42324..42476)
                     /locus_tag="ACHOWS_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFQ0046527.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            42751..43053
                     /locus_tag="ACHOWS_000900"
     CDS             42751..43053
                     /locus_tag="ACHOWS_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFQ0046528.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNVKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            43056..43415
                     /locus_tag="ACHOWS_000901"
     CDS             43056..43415
                     /locus_tag="ACHOWS_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0046529.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(43462..43854)
                     /locus_tag="ACHOWS_000902"
     CDS             complement(43462..43854)
                     /locus_tag="ACHOWS_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0046530.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            43981..44241
                     /locus_tag="ACHOWS_000903"
     CDS             43981..44241
                     /locus_tag="ACHOWS_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0046531.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            44238..44675
                     /locus_tag="ACHOWS_000904"
     CDS             44238..44675
                     /locus_tag="ACHOWS_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFQ0046532.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            44762..45772
                     /locus_tag="ACHOWS_000905"
     CDS             44762..45772
                     /locus_tag="ACHOWS_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFQ0046533.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEVYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSELHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAIQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWR
                     DD"
     gene            45765..46226
                     /locus_tag="ACHOWS_000906"
     CDS             45765..46226
                     /locus_tag="ACHOWS_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFQ0046534.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISGSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            46260..46976
                     /locus_tag="ACHOWS_000907"
     CDS             46260..46976
                     /locus_tag="ACHOWS_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFQ0046535.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLIKAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPVADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEDIKTETVADIVQSLPSFTETRADDLVLPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDILRDITATRELLR"
     gene            47009..47290
                     /locus_tag="ACHOWS_000908"
     CDS             47009..47290
                     /locus_tag="ACHOWS_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFQ0046536.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            47287..47592
                     /locus_tag="ACHOWS_000909"
     CDS             47287..47592
                     /locus_tag="ACHOWS_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFQ0046537.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            47579..48181
                     /locus_tag="ACHOWS_000910"
     CDS             47579..48181
                     /locus_tag="ACHOWS_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFQ0046538.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLADVRAEVFND
                     LCAAFVRHAKVAGLDDADTVTFKEATEALLHCAEQLRAPE"
     gene            48277..48459
                     /locus_tag="ACHOWS_000911"
     CDS             48277..48459
                     /locus_tag="ACHOWS_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046539.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            48452..48637
                     /locus_tag="ACHOWS_000912"
     CDS             48452..48637
                     /locus_tag="ACHOWS_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046540.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGEDDED"
     gene            48743..48946
                     /locus_tag="ACHOWS_000913"
     CDS             48743..48946
                     /locus_tag="ACHOWS_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046541.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVDKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            48939..49178
                     /locus_tag="ACHOWS_000914"
     CDS             48939..49178
                     /locus_tag="ACHOWS_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046542.1"
                     /translation="MFKQNEKSIAQIAEYIPRACRGMQLQEAKARLEKKIALYINDGC
                     DAAVLNAAFAPALNSHTRESFFSCIAAQIHKGGNQ"
     gene            49175..49519
                     /locus_tag="ACHOWS_000915"
     CDS             49175..49519
                     /locus_tag="ACHOWS_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFQ0046543.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALSAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            49521..49964
                     /locus_tag="ACHOWS_000916"
     CDS             49521..49964
                     /locus_tag="ACHOWS_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046544.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VVYMWDSERKDIDAPGYYRTEHLVFAESSVKQWGGRVVPLYTAPPAPIVPAVLERLRA
                     IVADPRALPRRKEWVSGQQYSYVLLENVEAMVDEACRAAMLQGDK"
     gene            49964..50107
                     /locus_tag="ACHOWS_000917"
                     /pseudo
     CDS             49964..50107
                     /locus_tag="ACHOWS_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            50135..50665
                     /locus_tag="ACHOWS_000918"
     CDS             50135..50665
                     /locus_tag="ACHOWS_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046545.1"
                     /translation="MEEAKELHEDLVRSHISKALSGEKMKKKDLDADLCWIHGVIVQA
                     SWFVKASLEQNALTGNSQVIPDDGREQFETLIRFHADDKNHETLLLRANEGMNYRDPN
                     VDLAWIFWKSSREHILPGKSDNNPALGDQVSELTMLVKRLASSLKSVNKSSKLPDKAM
                     DYLKRNGLISAEDILR"
     gene            50963..51118
                     /locus_tag="ACHOWS_000919"
     CDS             50963..51118
                     /locus_tag="ACHOWS_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFQ0046546.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(51225..>51499)
                     /locus_tag="ACHOWS_000920"
     CDS             complement(51225..>51499)
                     /locus_tag="ACHOWS_000920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046547.1"
                     /translation="VIISFKTNSPPQEIQECILSEWQRTPLLATITSQKIGKYYSVLA
                     VGDNADIYKLADGSTIIDFYSLRGVLDPTNGKSKRIQGIKSCISTK"
BASE COUNT        13159 a        12456 c        13414 g        12470 t
ORIGIN      
        1 tccagaatgc caaaaaatag tatgaaaatt gtgctatcag ctaagaaaaa actaatccgc
       61 aataagaatg cgggttattt tttgtgcgta aatgttgcaa tgtaatattc cgtaacagga
      121 tgatcgttat ggcggggttg caaatagatt gcttgactta acaaacagat aacttgacag
      181 aaaggataaa aaaagagcca gcgggtgctg gctcttgaag acgctaaatt agaagctggc
      241 gttacgcgga gtacgtggga acggaatcac atcacgcacg ttttgcacac cagttacgta
      301 agcaatcagg cgttcaaaac caagaccgaa acctgaatgc ggaacagtac cgtagcgacg
      361 cagatcgcga taccaccagt aatcttcttt attcaggccc atttccagca tacgctcgtc
      421 cagcacgtcc agacgttctt cacgctggga gccaccaatg atctcaccga tgcccggagc
      481 cagaacgtcc atagccgcaa cggttttacc gtcttcgtta aggcgcatat agaacgcttt
      541 aatatctttc ggatagtttt taaccactac cggtgcttta aagtgttctt ccgccagata
      601 acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc gggttttcaa acttcctgcc
      661 gcagttttcg agaatggtca ctgcgtcggt gtaatccacc tgcgcgaaat cggcctcaat
      721 gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt tcagcgaaga atttcatgtc
      781 gtcagcgcgt tcttcgagaa ccgctttgaa gacatatttc agcatggctt cagccagacc
      841 cgcgatatcg ttcaggttag caaacgccac ttccggctcc agcatccaga attccgccag
      901 gtgacggctg gtgttggagt tttcagcacg gaaagtcggg ccgaaggtat aaattttgga
      961 caatgcgcaa gcgtaggttt cgccgttcaa ctggccagat acggtcagga aagactcttt
     1021 accaaagaag tctttgtcga aatccacttt gccctgatcg ttacgcggca gattttccag
     1081 atccagcgta gaaacacgga acatttcgcc tgcaccttcg gtatcagatg cggtaatcag
     1141 tggcgttgaa acccagaaaa atccctgctc gttaaagaag cgatgcagcg cctgcgccag
     1201 cgtatggcga acgcgcgcga cggcaccaat caggtttgtg cgcggacgca ggtgagcgac
     1261 ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc gggtaagtgt ctggatcttc
     1321 aacccaacca gcaacttcaa ccttgctggc ctgaatttca aattgttgcc cctggcccgg
     1381 cgacgccacg actttacccg tcacaatgac cgagcagccg gtggtcagac gcaagacgtc
     1441 ttcattgtaa ttgggcagag aattattgat gacagcctgt acaggatcaa agcaggaacc
     1501 gtcataaacg gcgaggaagg agatgccagc ttttgaatct cggcgggtac gtacccatcc
     1561 gcgcacggtg acttcgctgt caacggctac gcggccctgg agtacgtcgg ctacaggcac
     1621 aacgctcata atattctctc tgttaatagt cggaaaaaat aaacacttgt ccacccgtaa
     1681 atggggggat acctatgtta cctggcatct gcaatcagac aagcagaatt cgcaaatgca
     1741 gcgaaagatt tcggaattaa aggtaaaaaa agggagccga ttagctccct gatgatatta
     1801 actggctttt ttaatatgcg gaaggtcgaa cgctttgcgt agcgcccgaa caaacgcttt
     1861 atcgtggcag atagttttgc ctgggctgtc agaaagtttc gccactggtt taccgttaca
     1921 ctctaccaac ttaatgacaa tattcagggg ttttacctgg gggatatcgc aggtcaggcg
     1981 agtcccaata ccaaaactta attgcacgcg ggaagagaag tggcggtata gctcaaccgc
     2041 tttgcgtaaa tccagattgt cagagaaaac cagcgtttta ctctgtggat caattcccag
     2101 cttttcataa tgtgcaatgg ctttttcacc ccattcaacc gggtcaccag agtcatggcg
     2161 caggccctga tagcgactgg cgaactcgac accgaaatct cgcaggaaag catccatagt
     2221 gatgcagtcg gttaacgcaa tgccaagttg gtcgggatac tcttccagcc aggcagcaag
     2281 tgcagctcgc tggctgttgg ctagatccgg gctgatttgc tgatgcgcct ggaaccattc
     2341 gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc gccagatcgt agttgctggt
     2401 gcccacgaac caggattcct gttgcagacg tttcacgatg gtttcttgta cttcacgaga
     2461 aaaacgtcgg cgggtgccaa aatccatcag atggaagcgc gacatatcaa gaccggcggt
     2521 taatgccgag aagtcggcta atttgctttc cagcgtgtcg agggcttgcg caacgtcggc
     2581 ctgcggtgag cgatagcgat gtaccatttc actgataacc gccagcaaag gaacttccca
     2641 gaggatgact tcacgccacg ggccgcttaa acgaatatcc agcttgccat tatcgttgga
     2701 cacggtgact tgttccgggt taaagcggaa ctcgcgtagc cagttaagat agtcggcctt
     2761 aaagaaaggc agggcagaga gccactgata ttcatcatcc tgcaggcgca ggtactgcat
     2821 cgcctgaacc tgttcacgaa tagcatcggc ataaataccc agcagatcgt cacctcggca
     2881 acgaaactcc gccgcgacat gcacatcgta atagtgatga aacacggctt gctgcatatg
     2941 caacttataa gcatctgtat ccagcaacga gtgcagaaca ggagaagcga attgtgtcat
     3001 aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca ataaacatct tcaggaactg
     3061 ctggagtata ccttgtttcg caatttattg aaccccgatc acaccatatg ccacctttct
     3121 ggtcgatggc attacgcggt gtatgttata aaaatgtagc aataaaggcg tttgtacctg
     3181 aaaagatgaa gattctgcat agcgcgattt acgcaacagg aatagactga acaccagact
     3241 ctataaaaga tgctaaaggt tatttatgac tcaacagcca caagccaaat accgtcacga
     3301 ttatcgtgcg ccggattacc agattactga tattgacttg acctttgacc tggacgcgca
     3361 aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat ggtgcatcag atgttccgct
     3421 tcgtctcaac ggcgaagacc tcaaactggt ttctgtttat attaatgatg agccgtggac
     3481 cgcctggaaa gaagaagagg gcgcactggt catcagtaat ttgccggagc gttttacgct
     3541 taagatcgtt aatgaaatta gcccggcggc gaatactgcg ctggaagggc tttatcagtc
     3601 aggcgatgcg ctttgcaccc agtgtgaagc cgaaggtttc cgccatatta cgtattatct
     3661 cgaccgcccg gatgtgctgg cgcgttttac caccaaaatt attgccgata aaaccaaata
     3721 tcccttcctg ctttccaacg gtaaccgcgt tgcgcaaggt gaactggaaa atggacgtca
     3781 ttgggtacgg tggcaggacc cgttcccgaa accgtgctac ctgtttgcgc tggtggcagg
     3841 cgactttgat gtgctgcgcg acactttcac cacgcgttct ggtcgcgaag tagcgctgga
     3901 gctgtatgtc gatcgtggca accttgatcg tgcgccgtgg gcaatgacct cgctgaaaaa
     3961 ctccatgaaa tgggatgaag aacgcttcgg cctggagtat gacctcgaca tctatatgat
     4021 cgtcgcggtg gatttcttca atatgggcgc aatggagaat aaggggctga atatctttaa
     4081 ctccaaatat gtgctggccc gcaccgacac cgccaccgac aaagattacc tcgatattga
     4141 acgcgttatc ggccatgaat atttccataa ctggaccggc aaccgtgtca cctgtcgcga
     4201 ctggttccag ctcagtctga aagaaggttt aaccgtcttc cgcgatcagg aattcagctc
     4261 tgaccttggt tcccgcgcgg taaaccgtat caataatgtg cgcaccatgc gcggattgca
     4321 gtttgcagaa gacgccagcc cgatggcgca cccaattcgc ccggatatgg tcattgaaat
     4381 gaacaacttc tacaccctga ccgtttacga gaagggcgcg gaagtgattc gtatgatcca
     4441 cactctgctt ggcgaagaaa acttccagaa agggatgcag ctctatttcg agcgtcatga
     4501 tggtagcgca gcgacctgtg acgactttgt gcaggcgatg gaagatgcgt cgaatgtcga
     4561 tctttcccat ttccgccgtt ggtacagcca atccggcacg ccggttgtga ctgtcaaaga
     4621 cgactacaat ccggaaactg agcagtacac cctgaccatc agccagcgca cgccagctac
     4681 gccggatcag gcagaaaaac agccgctgca tattccgttt gccatcgaac tgtatgataa
     4741 cgaaggcaaa gtgatcccgt tgcagaaagg cggtcatccg gtgaattcgg tgctgaacgt
     4801 cacccaggct gaacagacct ttgtttttga taatgtctac ttccagccgg tgcctgcgct
     4861 gctgtgcgaa ttctctgcgc cagtgaaact ggaatataag tggagcgatc agcaactgac
     4921 cttcctgatg cgtcatgcgc gtaatgattt ctcccgttgg gatgcggcgc aaagcctgct
     4981 ggcaacctac atcaagctaa acgtcgcccg tcatcagcaa gggcagctgc tgtctctgcc
     5041 ggtgcatgtg gctgacgctt tccgcgcggt gctgctcgat gagaagattg atccagcgct
     5101 ggcggcagaa atcctgacgc tgccttctgt caatgaaatg gctgaactgt tcgatatcat
     5161 cgacccgatt gctattgccg aagtacgcga agcactcact cgtactctgg cgactgaact
     5221 ggcggatgag ctactggcta tttacaacgc gaattaccag agcgagtacc gtgttgagca
     5281 tgaagatatt gcaaaacgca ctctgcgtaa tgcctgcctg cgcttccttg cttttggtga
     5341 aacgcatctg gctgacgtgc tggtgagcaa gcaataccat gaagcaaaca atatgaccga
     5401 tgcgctggca gcgctttctg cggcggttgc tgcacagctg ccttgccgtg acgcgctgat
     5461 gcaggagtac gacgacaagt ggcatcagga tggtctggtg atggataaat ggtttatcct
     5521 gcaagccacc agcccggcgg cgaatgtgct ggagacggtg cgtggcctgt tgcagcatcg
     5581 ctcatttacc atgagcaacc cgaaccgtat tcgttcgttg attggtgcgt ttgcgggcag
     5641 caacccggca gcgttccatg ccgaagatgg cagcggttac cagttcctgg tggaaatgct
     5701 taccgacctc aacagccgta acccgcaggt ggcttcacgc ctgattgagc cgctgattcg
     5761 cctgaaacgt tacgatgcca aacgtcagga gaaaatgcgc gcggcgctgg agcagttgaa
     5821 agggctggaa aatctctctg gcgatctgta cgagaagata actaaagcac tggcttgata
     5881 cttttgagcc aaaatgccgg atgcgacgct tgacgcgtct tatcaggcct acaagtccgt
     5941 tgaaaatgct ggttttgtag gctggataag gcgttcacgc cgcatccggc agccgtgccc
     6001 aaatgcctga tgcgacgctt gacgcgtctt atcaggccta caatccgttg aaactgctgg
     6061 ttttgtgtgc cacatccggc atattaacca tgacgccgaa tcggttgttc agagtgccca
     6121 cgctgcatca cccgttgcaa aacttccgct tccagctccg ctaatcgcac cgaccctaac
     6181 cggcgcggac gggggatatc caccgtcaaa tccagaccaa tttttccctc ttcaattaac
     6241 aatacccggt cagccattgc taccgcttcg ctcacatcat gcgtcaccaa cagtacggta
     6301 aagccgtgct cctgccaaag tgacacaatc aaatcctgca tctcgagccg agtcagggcg
     6361 tccagcgccc cgagcggttc atcaagcaat aataaacccg gtcgatgaat taacgctctt
     6421 gccagcgcca cacgctgttt ctgcccgcca gaaagtgcgg caggccattc ccctgcgcgg
     6481 ttctccagtc ctactgcagc caacgcccga cgcgcggcat cgcgccactg gcctttaagg
     6541 cctaacccaa cgttatcaat caccgatttc cacggtagca gacgcgcatc ctgaaacatc
     6601 attcgcgtgt cttcctgaat ctcagccagc ggtgtggtgc ccgctaacac gtcgcctgcg
     6661 gttggcgttt ccagacctgc cagcaggcgc agcagggtac ttttgccacc accgctgcgg
     6721 cccaccaccg ccacaaactg acctgccgga atatgtaaat ccagttggtt caggacgata
     6781 ttttccgcgt attgtttgct tactgcattg agcaacaatg gtgtgccctg gttcagacga
     6841 gcagtattca taccgtggcc tccttcaaat gataagccgg gttccagcgc aaccagagac
     6901 gctctaacag ctgcgcgctg acgtcagcaa gtttgccgag cagggcgtaa agaataatag
     6961 cgactaccac cacgtccgtt tgcagaaact cccgcgcatt catcgccaga taaccaatgc
     7021 cggaattggc agaaatagtt tcggcaacga tcagcgtcag ccacatcagg cccaaagcaa
     7081 aacgcacgcc gaccataatt gagggcaggg cgccaggcag gatcacatgg ataaacagcg
     7141 gtatgccgga taatccatag ctacgcgcca tttccaccag accgcgatcg atattacgga
     7201 tgccgtgcca ggtgttgata taaatgggga atagcgtgcc gagcgccacc agaaagattt
     7261 ttgcggattc atcaatgcca aaccacaaaa tcaccagcgg gatcagcgcc agatgcggca
     7321 cgttacgcaa catctgaatg gaggtatcca gcagtcgttc tccccagcga gatagcccgc
     7381 taatcagtcc caaaatcaac cccagcgatc cgccaattga aaagccaatg agcgcccgcc
     7441 aggagctgat cgccagatgc tgccacaatt cgccgctggc ggagagcgtc cagaacgcca
     7501 tcaccactcc ttccggtgag ggcaaaatac gcgttgacag ccagccaact gaggaggcca
     7561 gttgccacac cgccacgatg cccaccggta aaaaccaggg ggcaacgcgc aataaccact
     7621 ttttcactgg cgttgccatt atcgtattcc ttagctttgc gcgacgttac gggggataaa
     7681 atcatttgcc accgcttcgc cttgcggatt cagcggctgc ggctggggaa tttccgggat
     7741 ggcgacatcc agatgcggga acagcaactc gccaacccga tacgcttctt ccagatgcgg
     7801 gtagcccgaa agcacaaagc tgtcgatgcc aagcgcggcg tattcgttga ttcgcgcagc
     7861 gaccgtcgga ccatcgccca ccagtgccgt cccggcaccg ccgcgcacta agccaacgcc
     7921 cgcccataaa ttggggctga tctccagatt gtcgcgcttg ccgttatgta gcgccgccat
     7981 tcgctgttgt cctacggaat ccgtccgggc gaatgcggct tgtgctttgg cgatagtctc
     8041 atcatcaaga tgcgagatta accgctcggc ggcctgccac gcttcgtcgt tggtttcacg
     8101 aacaatcaca tgcagacgaa taccgaaacg aattttgcgt ccatgcgcgg cagctttcgc
     8161 ccgcacttgt tcgattttct ctttaaccag ttccggcggt tcgccccagg tgaggtagag
     8221 atcaacttgt tctgccgcca gctcctgggc gacatctgac gatccgccaa agtaaagcgg
     8281 aggatacggc tgttgaatcg gcgggaagag cagtttggct ccgcgcacat gaatatgttt
     8341 accgttgaaa ttgacggttt cccccagcaa taagcgccgc cagacctggg taaattccgc
     8401 cgaggcttcg tagcgctcgc tatgatcaag gaacacgccg tcgcctgcca gctcttgtgg
     8461 atcgctgcct gtgaccagat taaacaacgc acgaccattc gagagacggt caagcgtggc
     8521 ggcctggcgg gcggcaacgg taggtgaggt tacgctggga cgcagggcga caagaaactt
     8581 cagccgttgc gtcaccggga tcatcgacgc ggcaaccagc cacgcatctt cacacgatcg
     8641 tcctgttggg atcagcacac cggtataacc cagacgatcc gccgcttgcg caatttgttg
     8701 cagataaccg tgatcaaccg gacgtgaacc ttcttccgtt cccagatagt gcccgtcacc
     8761 atgggtcggt aaaaaccaga acatattcag actcataatt gttttccttc cagttgagtg
     8821 ggctgccaga tgcgctggcg aatatcgact tttttcggca ccagacggtt ttcataaaac
     8881 agatctgccg tttgctgctg taaggcggca acttcagcgc tcaacggttt gatggtggtg
     8941 ggaggacgat gatccagata tgaggcaata accggtgccg gtaagcccat cgtttttgcc
     9001 agtaaagcga tgctttgttc gcgctggctg cgggttaacg catcggcctc actaaaggtt
     9061 gtcagcacac cctgaataaa agcgccgttt ttttctgcat acggacgagc tgccagataa
     9121 aacgatccag tttgattgag atcggtgccg tctttcagca cccgcacgcc gccctgtaat
     9181 aatgcagcgg agtagtaggg atcccagata gcccaggcgt caacgttacc ttgctggaac
     9241 gcggcgcggg catcggcagg cgtcaggtaa gtgggctgaa tatccgtgaa tttaagcccg
     9301 gcctggcgaa gggctcgcag taaaagattg tgcgaactgg aacctttctg aaaggcaact
     9361 ttgtgacctt taagatcggc tacggttttg atcgggctgt tttctgccac cagaatcact
     9421 tcggctttgg gtttcggtgg ctcgacgccc acgtatacca aatcagcccc ggcagcctgg
     9481 gcaaagattg gcggaatatc cccggtactg ccgagatcga tactgccaac gtttaacgcc
     9541 tccagcatct gtgggccagc ggggaactcc acccaggaga tttttgtttg cggatagcgt
     9601 ttttccagta actggtggct ttttgccagc accataccaa tactgccttt ttgatagcct
     9661 atacgtaacg cctctggcga agactccgcg gcaacagcaa gtgtagagac gctaagtaaa
     9721 cccgccagcg ccagtttaat gatcttacgc atgggcatta cctcgcagag acagaaggtc
     9781 aggaacctga acatcgcggc ggtgcaacgc ctgccagaaa gtttctagcg cggtatcaag
     9841 acgagtttgc agatttggcg tgaactgggg tttgtgttgg taatcaatta cttgcgagtc
     9901 atcggcaaac acgccgtgca ggatctcctg ggctttcagc gtacttaaaa ctggcttaag
     9961 ggcataatcg accgccagca gatgggccac ggtgccgcct gtcgccagcg gcagcaccac
    10021 tttgccttgc aacgcacgtt ctggcagcag gtcgagcagg gttttcagcg cgccggaata
    10081 ggcggcttta tacacaggcg tggcgacaat cagcccatcg gcctgttgca gctgttcggt
    10141 gaaggtcttg agtgccggac tatcgaaacg agcataaagc agatcttccg ggacgaagtt
    10201 ttgcaaattc cagtgataaa cctctacatc caggccattt agtttttccc gcgcatattc
    10261 cagcaaggag ctggagcgag aaggaaagcg aggactaccc gccagggtga tgacacgcat
    10321 actctctcct tataaccaat tgttcttgtt ttgttaacat tgataacaat tcggtcagtc
    10381 tgacagaggg cgtccgcgag tcgaaatgat ttatctgagg aagaaaagcc aaaaaaagca
    10441 taaaaaaagc gcaataagaa aacgtttgct atttattgcc gcaggtcaat tcccttttgg
    10501 tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga gagttcatgt
    10561 actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct catgagttta
    10621 cttttcagca attacgccgt attaccggaa cgccgtttga agcactggtg cggcagaaag
    10681 tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag
    10741 ccggtcttga taaagacggg gagtgcattg acgcgttagg cgcgatggga tttggatcga
    10801 tcgagatcgg taccgtcacg ccacgtcccc agccaggtaa tgacaagccg cgtctctttc
    10861 gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt ggcgttgata
    10921 acctcgtaga gaacgtaaaa aaagcccatt atgacggcgt tctgggtatt aacatcggca
    10981 aaaataaaga tacgccggtg gagcagggta aagatgacta tctgatttgt atggaaaaaa
    11041 tctatgccta tgcgggatat atcgccatca atatttcatc gccaaatacc ccaggattac
    11101 gcacactgca atatggcgaa gcgctggatg atctcttaac cgcgattaaa aataagcaaa
    11161 atgatttgca agcgatgcac cataaatatg tgccgatcgc agtgaagatc gcgccggatc
    11221 tttctgaaga agaattgatc caggttgccg atagtttagt tcgccataat attgatggcg
    11281 tgattgcaac caataccacg ctcgatcgtt ctctggttca gggaatgaaa aattgtgatc
    11341 aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag cacagaaatt attcgccgct
    11401 tgtcacagga attaaacggt cgcttaccga tcatcggtgt tggcggcatt gattcggtta
    11461 tcgctgcgcg tgaaaagatt gctgcggggg cctcactggt gcaaatttat tctggtttta
    11521 tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta tttcttcgaa
    11581 ttcctcttta taagataatc agggctttat tttaagccct ggttgtttta tattcatcgc
    11641 tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga agcgacaatg
    11701 gattgtgttg ctgcggtttt atagggtagg ggagaggcag atgcgaatta aaccagacga
    11761 taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt tagccaatgg
    11821 tatgctattt cgctcacgtt ttgcgcgcaa aatgttgact ccagatgcct tttctcccgc
    11881 aggcttttgc gttgacgacg ccgcgctcta tttctcattt gaagaaaaat gccgcgattt
    11941 taatttatct aaagaacaaa aagccgaact ggtgcttaat gcgctggtag caattcgtta
    12001 tctcaaaccg caaatgccga aaagctggca ttttgtttcc catggtgaaa tgtgggtgcc
    12061 catgccgggc gatgcggcct gcgtttggtt gagcgacact cacgagcaag tcaatttgct
    12121 ggtcgttgaa tctggcgaaa atgccgcact atgcctgctg gcacaaccct gcgttgttat
    12181 tgcgggtcgc gcgatgcagc tgggcgacgc catcaaaatc atgaacgaca ggctgaaacc
    12241 gcaggttaat gttgacagct tcaacctcga acaggcagtc taacgcgtca gcttaagcgc
    12301 agtcttcggt acacagctac agcaaagaat ggtgccatca tcgcccattg ctgatttttt
    12361 cagcggcgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa tgcccgcgcg
    12421 gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcaaca cctgctggtt
    12481 atttccacga aatgcctgtc cctgccagtc aatatctaca tttgcgtccg gttgttgcgt
    12541 gatgttgacg gtatcatcag cggcggctgc gccgtaaatt ttagccggag ccgttgccag
    12601 aacttccacc tcatcgccaa cgcgaattac gccgctatta cgggcaatta aattctgacc
    12661 aaaatcgaca tcgccgttat cctgggcagt gcggaaagat tgcaatgttt ttaatggttc
    12721 gcctgccgga tgtttttgcc ctttttctgg actgacggtg gtgaaaatac agcggctaca
    12781 aggtttaacc acatcaaaca ccacatcacc aatgcgaatc actttccagc tatcttcttc
    12841 ccaggctgac gcgccggaaa ccaccagatt ggggcggaat tgctccattt ttacactggc
    12901 cggacaacgt tgttggagat cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc
    12961 atcagcaaat gacaggggta cagtgttgtg gcgtttcacg cgccgcgtca tttgtggccc
    13021 cacccagcgc aactgcactt cgcgggagaa aaatccactt agccatttgt tgatcgcgtc
    13081 tggtgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc
    13141 gaaatcagca aaacgaacat atgcgctact gccatctggt gcggtgagat gcaagccatc
    13201 atgcacaggt gaaggagtaa accgtaccat ctggggaaac tggcgagcgg taataaacgt
    13261 accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga catctgccag
    13321 ggcatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa gccgggttaa
    13381 tgtcgccacg gtgcggtcct caaatgaaaa taagccctca actttatgac atgacgcgct
    13441 tattagctat aatgcgcaac aattttctta gcgcctgtcc cattaggcta ttttatttgt
    13501 cagtttggcc cagggaagtg ctcgaaatcc tcacgtactt gtgtacgctc cggtttctcc
    13561 gcgctgtcca tgtccagact gactgcaaca attacgccta ctgcggtagg ctcttagagt
    13621 aaaagtgacg atatgaattc tctgtttgcc agtacggccc gtgggctgga agagctgtta
    13681 aaaactgaac tggaaaacct gggggccgtt gaatgccagg tggttcaggg tggggtccat
    13741 ttcaagggcg acacacggct tgtttaccag agcctgatgt ggagccgcct ggcctcgcgt
    13801 attatgttgc cgctgggcga gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt
    13861 caggcgatca actggacaga gatgtttaat cctggcgcga ccttcgctgt ccacttcagt
    13921 ggtttgaatg acaccatccg caacagtcag tacggcgcga tgaaagtgaa agacgcgatc
    13981 gtcgatgctt tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat
    14041 atccgcgtta acgtctggct gcataaagaa accgccagta ttgcccttga tctcagtggt
    14101 gatggtttac atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc
    14161 ctggcagccg cgattgttat gcgatccggc tggcagccag gaacaccgct gctcgatccg
    14221 atgtgtggtt ccggtacgtt gttgattgaa gcggcgatgc tggcgaccga tcgcgcacca
    14281 ggcttgcacc gtggacgttg gggctttagc ggctgggcac agcatgatga agccatctgg
    14341 caggaagtga aagcagaagc gcaaacccgc gcccgtaaag gcctggctga gtatagctct
    14401 catttttacg gttcggacag cgacgcacgg gtgattcaac gtgcgcgcac taacgcccgt
    14461 cttgcgggga ttggtgaact gatcaccttt gaagtgaaag atgtcgcgca actgaccaat
    14521 ccgctgccga aagggccgta cggtacagtg ttgagcaacc cgccatacgg tgaacgtctg
    14581 gacagcgaac cggcgctgat tgcgctgcat agtctgctgg gccgaatcat gaaaaaccag
    14641 ttcggcggct ggaatctctc tttattcagt gcctcgccgg atctgttaag ttgcctgcag
    14701 ttgcgtgcag acaaacagta caaagcgaaa aacggtccat tagactgcgt gcagaaaaat
    14761 taccacgttg ccgaaagcac accagacagc aaaccggtaa tggcagcgga agactacgcc
    14821 aaccgtctgc gtaagaacct caaaaaattc gagaagtggg cgcgtcagga aggcattgaa
    14881 tgctaccgcc tgtacgacgc cgatctaccg gaatacaacg ttgcagttga ccgctacgcc
    14941 gattgggtgg tggtgcagga gtatgcgccg ccaaaaacta ttgatgcgca caaagcgcgt
    15001 cagcgtctgt tcgatattat cgctgcaacc atttcggtat tggggattgc gccaaacaaa
    15061 ctggtgctga aaacccgtga acgccagaag ggcaaaaatc agtaccagaa actgggcgag
    15121 aagggcgagt ttctcgaagt taccgaatat aacgctcact tgtgggtgaa cctgacggat
    15181 tacctcgata ccggtctgtt cctcgatcac cgtatcgccc gtcgtatgct cggtcagatg
    15241 agcaaaggca aagatttcct caacctgttc tcttataccg gcagcgccac cgtgcacgcg
    15301 ggattaggcg gtgcacgcag cactaccacc gtggatatgt cgcgtactta tctggagtgg
    15361 gcagaacgca acctgcgtct gaatggccta accgggcgtg cgcatcgcct gattcaggcc
    15421 gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg atctgatttt tatcgatccg
    15481 ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg cgatcatctg
    15541 gcgctgatga aagatttgaa acgtctgctg cgtgctggtg ggacgatcat gttctcgaac
    15601 aacaaacgtg gcttccgtat ggatcttgac ggactggcaa aactgggact gaaagcacaa
    15661 gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta accgccagat ccacaactgc
    15721 tggctgatta ccgcagcctg aaaggaatag taatgtcatt aatcagtatg catggcgcat
    15781 ggctgtcgtt cagcgacgcg ccgcttctcg ataacgcaga actgcatatc gaagataacg
    15841 aacgtgtttg tctggtgggc cgcaacggcg caggcaaatc gacgttaatg aaaatcctca
    15901 accgtgaaca agggctggat gacggtcgca ttatttacga gcaagatttg attgtagctc
    15961 gtctgcaaca ggatccgccg cgtaacgttg aggggagtgt ttatgatttc gttgccgaag
    16021 gcattgaaga acaagcggaa tacctgaaac gctatcacga tatttcgcgc ctggtgatga
    16081 acgacccgag cgagaaaaat ctcaacgaac tggcgaaggt tcaggaacag ttggatcacc
    16141 acaacctgtg gcagctggaa aaccgcatta acgaagtgct ggcgcaactg gggttagatc
    16201 ctaacgttgc gctgtcgtcg ctttccggcg gctggttgcg taaagcggca ttaggacgcg
    16261 cgctggtgag caatccgcgc gtattgctgc tcgacgaacc gactaaccac ctggatatcg
    16321 aaaccatcga ctggctggaa ggattcctta aaaccttcaa tgggacgata attttcatct
    16381 cccacgaccg ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc gatcgcggca
    16441 agcttgtgac ctatcctggg aattacgacc agtacctgct ggaaaaagaa gaagccctgc
    16501 gcgtggaaga actacaaaat gccgagttcg atcgcaaact ggcgcaggaa gaggtgtgga
    16561 tccgccaggg gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc gccctgaaag
    16621 caatgcgtcg tgagcgtggc gaacgtcgcg aagtgatggg taccgcaaag atgcaggtgg
    16681 aagaggccag ccgctccggt aagatcgttt tcgaaatgga agacgtttgc taccaggttg
    16741 acggtaagca actggtgaaa gatttttctg cccaggttct acgtggcgac aaaattgccc
    16801 tgattggtcc gaatggatgc ggcaaaacca cgctgttgaa actgatgctc ggtcagcttc
    16861 aggcggacag cgggcgtatt cacgttggca ccaaactgga agtggcttat ttcgatcagc
    16921 accgtgcgga actggatccc gataaaacgg tgatggataa ccttgctgaa ggtaagcaag
    16981 aggtgatggt taacggcaag ccacgtcatg ttttgggcta tttgcaggac ttcctgttcc
    17041 atccgaaacg ggcgatgacg ccggtacgtg cgctttctgg cggtgagcgt aaccgcttgc
    17101 tgttggcgcg tttgttcctc aaaccaagca acttattgat tcttgacgaa ccgaccaacg
    17161 atcttgatgt tgaaacgctg gaactgctgg aagaactgat cgacagctac cagggcacgg
    17221 tattgctggt gagccacgat cgtcagtttg tcgataacac cgttacagaa tgctggatct
    17281 tcgaaggcgg cggtaaaatt ggtcgttatg tcggcggtta tcatgatgcc cgtggtcagc
    17341 aagaacagta tgtggcgctc aaacagcctg cggtgaaaaa aatcgaagaa gccgccgcgc
    17401 caaaagcaga aactgtaaaa cgcagcagta gcaaactaag ctataaattg cagcgcgaac
    17461 tggagcagct accgcaattg ctcgaagatc tggaggcgaa gctggaagcc ctacagacgc
    17521 aggtggcgga tgcttccttc ttcagtcagc cgcatgagca gacgcaaaaa gtgctcgccg
    17581 atatggctgc tgcagagcag gagctggagc aagcctttga acgctgggag tatcttgaag
    17641 cgttaaaaaa tggtggctga tcgcaaaata gtcgatttaa acagagacag ctgacaacgg
    17701 taaaattgcc tgatgaactg ggtttatcag gcatgaaaac caacactttg ttagtcatct
    17761 gtaaaatagc gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc gcgaagcaca
    17821 tcctgtgctc gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat ggtcagaaag
    17881 cggcatgtcc ccggtgtggc acaacgttaa ccgtggcgtg ggatgcccct cggcagcgtc
    17941 ccaccgccta tgcgttggct gcactgttca tgctgttgct gtccaacttg tttccttttg
    18001 tgaatatgaa cgttgcggga gtcaccagtg aaattacatt actggaaatt cccggcgtgc
    18061 ttttttctga ggactacgcc agcctcggca cctttttcct gttgtttgtg caactggttc
    18121 ccgcgttttg tctgataacc attctgttac tggtgaatcg cgcagaatta ccggtccgtt
    18181 taaaagagca actggcacgg gtgctttttc aactcaaaac ctggggaatg gcggagattt
    18241 tcctcgcggg tgtgctggtc agtttcgtta aactgatggc ttacggcagc attggcgtag
    18301 gcagcagctt tctcccctgg tgtttatttt gtgtcctgca actgcgtgct tttcagtgcg
    18361 ttgatcgtcg ctggttatgg gacgacatcg ccccgatgcc agaactgcgc cagccgctaa
    18421 aacctggcgt cacggggata cgccaggggt tacgttcgtg ctcctgttgt acagcaattc
    18481 ttccagctga tgatccggtg tgtccgcgct gtggtactaa agggtacgtt cgtcgtagaa
    18541 acagcctgca atggacactc gcgctgcttg ttacgtccat catgctgtat cttccggcta
    18601 atattttgcc catcatggtg acggatttat taggctcgaa gatgccatcg acgattctcg
    18661 ctggggtcat cctgttatgg agcgaaggat cttatcccgt cgctgcggtt atctttctgg
    18721 ccagtattat ggtgccaacg ttaaagatga tcgccatagc gtggttgtgt tgggatgcca
    18781 aagggcatgg caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt gttgagtttg
    18841 taggccgctg gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg ctggtgcgta
    18901 tgggaggttt aatgagtatt tatccggcaa tgggtgcatt aatgtttgct ttagtcgtca
    18961 taatgacaat gttttctgct atgacgtttg acccgcgttt gtcgtgggat cgtcaacctg
    19021 aatcagagca tgaggagtcc tgacagtatg gaatctaata atggggaagc caaaatccag
    19081 aaagtgaaga actggtctcc cgtgtggata tttcctatcg tcacggcgct cattggggcc
    19141 tgggttcttt tttatcatta cagccatcag gggccggaag tgaccctgat caccgcgaat
    19201 gcggaaggaa tagaaggtgg taaaaccacc attaaaagcc gtagcgttga cgtcggcgtg
    19261 gttgaaagcg ccacactggc tgatgatttg acgcacgttg aaatcaaagc gcggctgaat
    19321 tccggtatgg aaaaattgct gcataaagat accgtctttt gggtggtgaa accgcagatt
    19381 ggtcgagaag ggattagcgg cctgggaacg ctgctgtctg gtgtttatat cgaactgcag
    19441 ccaggcgcga aaggcagcaa aatggataaa tacgatttgc tggactcacc accgttggcc
    19501 ccgcctgatg cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg gcagctctcg
    19561 ccaggagatc ccgtgctgtt ccgtggctat cgggtaggtt cggttgaaac cagcaccttt
    19621 gatacgcaaa aacgcaatat cagctatcaa ctgttcatca atgcacctta tgaccgactg
    19681 gtgaccagca atgttcgctt ctggaaagat agtggcattg cggttgattt gacgtcagcg
    19741 ggaatgcgtg tggagatggg ttcattgaca acgctgctta gtggcggtgt cagctttgat
    19801 gtgccggaag gtctggattt agggcagcca gtggcaccga aaacagcttt cgttttgtat
    19861 gatgatcaga agagcattca ggattcgttg tacaccgatc acattgatta tctgatgttc
    19921 tttaaagatt cggtacgcgg tctgcaaccg ggagctccgg tagaattccg gggtattcgc
    19981 ctgggtaccg taagcaaagt gccattcttt gcgccgaata tgcgtcagac atttaacgat
    20041 gattaccgta ttccggtact gattcgtatc gagccagagc ggctgaaaat gcagcttggc
    20101 gaaaatgcgg atgttgttga gcaccttggc gaattgttga aacgtggttt acgcggatcg
    20161 ctgaaaaccg gaaacctggt cactggcgca ctgtatgttg atctcgattt ctatccaaat
    20221 acgcctgcaa taaccggtat tcgtgaattt aatggttatc agattatccc gaccgttagc
    20281 ggcggtctgg cgcaaatcca gcaacgactg atggaagcgt tggataagat caacaaactg
    20341 ccattgaatc cgatgattga acaggcaacc agtacgcttt ctgaaagtca gcgcacaatg
    20401 aaaaacctgc aaacgacgct ggatagcatg aacaagatcc tcgccagcca gtcgatgcag
    20461 cagctgccga cggatatgca gtcaacgttg cgtgaattga atcgcagcat gcagggcttc
    20521 cagcctggct ccgcagccta caacaagatg gtggcggata tgcagcgcct tgatcaggtg
    20581 ttgcgagaac tgcaaccggt gctgaaaacg ctcaatgaga agagtaacgc gctggtattt
    20641 gaagcgaagg acaaaaaaga tccagagccg aagagggcga aacaatgaaa aagtggctag
    20701 tgacgattgc ggcactgtgg ctggccggat gcagctccgg cgaaattaat aaaaactatt
    20761 accagttacc tgtggtgcag agcggtacac aaagcaccgc cagccagggt aatcgcctgt
    20821 tatgggttga gcaggtcgct gttcctgact atctggcggg gaatggtgtg gtttatcaaa
    20881 ccagtgatgt gaagtatgtg attgccaaca acaacctgtg ggccagcccg ttggatcaac
    20941 agttgcgcaa cactctggtt gccaacctga gcacgcaact gcccggctgg gtggttgcct
    21001 cccagcctct gggaagcgcc caggatacgc tcaatgttac cgtaacggag tttaacgggc
    21061 gatatgatgg caaggtcatt gtcagtggtg agtggctgtt gaaccaccag ggacaactga
    21121 tcaaacgtcc gttccgtctg gaaggggtgc aaactcagga tggttacgat gaaatggtta
    21181 aagtgctggc cagtgtctgg agtcaggaag ccgcttctat tgcgcaagag ataaagcgtc
    21241 taccgtaatc ataaagattt gtaaatataa ccgtctccgg tatgttgcct gaggcggttt
    21301 ttttgtttct aaggtgcgga aaaagttgtt cctcttcaca ttttttgtac aactgacatg
    21361 cccgtgtagc tcacaaatat gacagtggcg tgaattttgc gcattgacgg cagttatgat
    21421 tcgcggtatt gcttaactgt gattgcacat ttagtaatca ctgttttctt ttccaccaga
    21481 aaccagtatg agggaaacga ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac
    21541 atcaacgtgg ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga
    21601 cgctgaatca aaggtcacaa tggctgggag gctggcgaga agccatggcg gacagggtag
    21661 taatggcctg attctgtctc tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt
    21721 gatactctgt ctgattataa tcagaaggca gacgtatcct ggaacagacc gactttcagg
    21781 tcgctggcgg tatagatcag acgaccatca accagcactt cgccatctgc cagacccata
    21841 atcagacgtc gattaacaat gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc
    21901 ggcagtacct gaccggtgaa tttcacttcg ccaacgccca gcgcgcggcc tttaccttcg
    21961 ccgcccagcc agccgaggta gaaccctacc agctgccaca ttgcgtccag gcccaggcat
    22021 cccggcataa ccggatcgcc aatgaagtgg catccgaaga accacagatc cggattgata
    22081 tccagttctg cttcaacata ccctttgtcg aagttaccac ccgtttcggt cattttgacc
    22141 acacggtcca tcatcagcat gttcggtgct ggcaattgcg ggcctttagc gccaaacagt
    22201 tcaccgcgac cagaggcaag aaggtcttct tttgtatagg attcgcgttt atctaccatg
    22261 ttctctgtaa gccttatttt attgaagcac gcaggatagc taacacgtgt acgctgaaca
    22321 agtccgatca gttcggaata aaccagttca gccaacgcaa tggccatgga aaacggtgac
    22381 gtccttcctg ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt
    22441 ggccttcgcc atcccacacc agatttaata ataacggcag tgcgtcagtc acatcgtcta
    22501 ctgcccagat ggtgaatttg ccttcttcta ccgctttcac cagttcactg tgaagactta
    22561 aatggcgaat gttagcagtg gggataatga caccttgttt cccggttaac tcacgttgct
    22621 ggcaaatagc aaagaagcct tcgattttct catttaaacc accgaccggc tgggcacgac
    22681 cgaactgatc gactgaacct gtgatagcga tactctgatt caccggcaca tcggcgaggg
    22741 cgcttatcag ggcgcagagt tcagccatcg aggcactatc gccatccact tcactgtatg
    22801 actgctcaaa tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt agttccgaca
    22861 tcaggaacgc ctgcatgatc atcatccctt tcgcatggat attgccgcca agctccgctt
    22921 tgcgttcgat gtcggtgaac tcaccatcgc caatatgcac aacgcagcta atgcgagaag
    22981 gttcgccaaa agcgcgtgga tgacccggaa attcaatgac cgaaagggcg ttaatttgcc
    23041 cgatgcgttc gccttcggtt tcaatcagga tttgctcctg aagaatctca tcctgcatgc
    23101 gttcagcgag gaaaccttca cgccattcac gctgctgcag cattaagttt agctgctcgc
    23161 cggagaaggt gtcgccatcg cacagggagg cgacctcttt acactggcgg aggatccact
    23221 gcgggctaag cggaagtgtt tcttgttcac cggtgtagcg ggctgcttcg cggataagta
    23281 ccggccaggc atccgctccc ggtgcaggta agtgattatg tcttgcggtc aatgttaccc
    23341 agcgacacca ctggcttact gactccgcat cgacaatctg cagagtatct tcaaattcgc
    23401 tataaatagc ctgctctgaa agctctggtt ccatctcctg gaaatcagcc aatgattcgc
    23461 gttcgcctac cagaatgact ttcagcttca atggcatcga aggcacagag acggggagag
    23521 ggcgcgactc atcaaacgca acccagtcaa aacgctcgcg gttaacgata tttttcagcc
    23581 gcatccacag cagaggttgc gccagcagtg tacgcaaaga gatgatgaga ataccgccat
    23641 ttgcctgatg caccagacct ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc
    23701 caaagagttg ttccgcttct acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat
    23761 tatcatctgc actcactgcg ttacgtaagc ggatggtgtg cccggaaacc tcatagtgac
    23821 cgcccaccag ttggcctgca tcgctttgta gcgcacgcgc ggcatcggca ataagattca
    23881 gatactcaga ctcttccggg gccttcgcca gcataaaaga ggaggatgct cgcgtatgca
    23941 gcaactgctc cagcgcaaat tgcagtcgcg gttgagtatc actgaataaa ggatcgtttt
    24001 cgtcaatcaa atgtggctga gcaaatattt cctgatagct atcggtatca ggaaccaggt
    24061 cacgccatgc aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg
    24121 cgattatacc gtaaccggca ctacagcaca cgtagaaagc accgacaata ctcctggcat
    24181 gggcgttaaa gctcacagga tggagattct tttcttcact ggcccaaaaa gctgatattc
    24241 tgtaaagagt tacacggtaa cattgagatc gctatgaaat atcaacaact tgaaaatctt
    24301 gaaagcggtt ggaaatggaa gtatctggtg aagaagcacc gcgaagggga gttaatcacc
    24361 cgttacatag aagccagtgc cgcccaggaa gccgtcgatg tgttgctctc gctggaaaat
    24421 gaacccgtgc tggtaaatgg ctggattgac aagcatatga atccggaact ggtcaatcgc
    24481 atgaagcaaa ccattcgggc cagacggaag cgccatttta atgcggaaca tcagcatacg
    24541 cgcaaaaaat cgatcgatct ggaatttatt gtctggcaac gtctggctgg tcttgcgcag
    24601 cgtcgcggta aaacgctttc tgaaacgatt gttcagctga ttgaagatgc ggaaaacaaa
    24661 gagaaatacg cgaataaaat gtcttctttg aagcaggatc tgcaggcatt gctgggtaag
    24721 gaataactga cgaaagtcag ttcaattttc taaagacgaa aaaaaacccc gcagcagcgg
    24781 ggttttttac cagacgagaa cttaagcctg cggctgagtt acaacgtctt tgatgccttt
    24841 aacttcgatc tctacgcgac gatccggagc caggcaatcg atcagtgcag cacgctgttt
    24901 cacgttgtca caggtgttgc cagtaaccgg gttggattcg cccataccac gtgcggagat
    24961 tttgtctgcc gggatacctt tagagatcag gtaatcaaca acggactgag cacgacgctc
    25021 ggacagagcc tggttataag cgtcagaacc gatgcggtca gtgtaaccca gaacaactac
    25081 ggaaccgtct ttcggatcca ggttgctcag ctggctgtac agctgatcca gagcagcctg
    25141 accttccggt ttcagggttg ctttgttgaa ggtgaacaga acgtcagact tcagagtgaa
    25201 gtgcttggtc tgtacttccg gtgccggagc tggagccgga gcaactactg gagctgcttc
    25261 gccctgaccg aaacggtagg aaacacccag gctcagcatg ccgttgtccg gacgagtgcc
    25321 gatggtgtgt gcgtcaccga tgttgttggt ccactggtat tccagacggg tagcgatttc
    25381 aggagtgatc gcatactcaa caccgccagc gaagaccgga gaaacgccgg tgtcgtggtc
    25441 tttagtggat gcgccaccag gtacgttaga cttggtgtct gcacgccata ccataccacc
    25501 cagacgagtg taaacgtcca gatcgtcagt gattgggtaa cccagtttag cggtcagctg
    25561 aacgccctga gctttgtatg cgccgttgat gttgtcgcct ttgtacggca tacgacctaa
    25621 ccagtcgtaa cccatttcaa agccaacata cgggttaacc tggtaaccac caaaagcacc
    25681 tgcacccagt tggttttcgt gggtcggacc attgttagga ataaaaccag tgtcatggta
    25741 ctgggaccag cccagtttag caccagtgta ccaggtgtta tctttcggag cggcctgcgc
    25801 tacggtagcg aaaccagcca gtgccactgc aatcgcgata gctgtctttt tcattttttg
    25861 cgcctcgtta tcatccaaaa tacgccatga atatctccaa cgagataaca cggttaaatc
    25921 cttcaccggg gatctgctca atattaactc taccgatatc ttcggcttat gccgagcacc
    25981 cctggcgatg taaagtctac aacgtagttg aaaacttaca agtgtgaact ccgtcaggca
    26041 tatgaaaaaa aagtcttgta taaggtacgt ttaatctttt ttgtcagcga caatttacag
    26101 aagaaaatcg cggaaaccgc ttcagacaag cctccgcaag gaaaattagt cacgactgaa
    26161 agcattggct gggcgacaaa aaaagttcca ggattaatcc taaatttact taatgataca
    26221 aattagagtg aatttttagc ccggaaagtt gtctcgttgc gtgagaggat gcgcttaccg
    26281 gacgcataat aaaccccata gcgttacctt catttgccgc atcaacaagt tcagcatgct
    26341 cttcttcagt caaatcatct gccaaccaac cgatcaccac gctgtaattg cccgtgcgta
    26401 aagcgcgaac cattgactca acggtgtggc aaggggagag ctggctaatc tgcattactt
    26461 tcgttaaggg tagcccagat gcctgaaccc attcgcgact cagtttttgt tgcggtgtta
    26521 accagagttg ccagcgcgat tgctgaccga gttgctgtaa caatggcaac agtagaagtt
    26581 gcgtcatcat gggctgatct tcgcgataga caacttcact gataagcccg gctgtagtgt
    26641 tttccgtaga gacacgcgca attttacttg ctgcggatga gaacgacgaa gcacgatgtg
    26701 catagcctga agtgtacata atcaatccag ccctgtgggt tactgtatga atatacagta
    26761 catccggtga cgacaaagat caaccctatt ttcggaaaga gcctcgcaaa ttttgtcgtt
    26821 agtgacggga aaacataaat taatcttgcc ccttaagaat aagttgccta ttttcgtagt
    26881 taacggatcc gttaatgtga atcattcttt tatgttatga ttttaaaagg aattttatga
    26941 aaagcctctc ctataagcgg atctataaat cacaagaata cctggcaacg ttgggcacaa
    27001 ttgaataccg atcattgttt ggcagttaca gcctgaccgt tgacgacacg gtgtttgcga
    27061 tggtttctga tggtgagttg tatcttcggg cttgtgagca aagtgcacag tactgtgtaa
    27121 aacatccgcc tgtctggctg acatataaaa agtgtggccg atccgttacc ctcaattact
    27181 atcgggttga tgaaagtcta tggcgaaatc aactgaagct ggtgcgtctg tcgaaatatt
    27241 ctctcgatgc tgcgctgaaa gagaaaagca cgcgcaatac ccgggaaaga ctgaaagatt
    27301 tgcccaatat gtcttttcat ctggaagcga ttctcgggga ggtggggatt aaggatgtac
    27361 gggcgttacg tatacttggg gcaaaaatgt gttggttgcg actgcggcag caaaacagtc
    27421 tggtgacaga aaagattctg tttatgcttg aaggggccat tatcggcatt catgaagctg
    27481 cgctcccggt ggcacgccgc caggagcttg cagaatgggc tgactctctt acgccgaaac
    27541 aggagtttcc tgcggaactt gagtaatctg gcgttgcaga cgaccaatct ctggcagtaa
    27601 tgcaatcaat aacccaactt gttgtacgac cagaggttct ttgctgtctg cccgtggttc
    27661 aagttgctgc atccgctgtt tcaggccagc taatgcctga ttgacgcgtt cttcatcagc
    27721 aggttgatga tgtaacgcgt catcaacata gcaaactgcg tcatcaagaa acgccagaat
    27781 ttcaggatta gttaactgct cccggtgagc accgagggct gagatatagc tggtaaacgt
    27841 atggttaagg cacagcaacc gaaacgcggc ttcgcgaatt tgcggggtaa cgttcggttc
    27901 gctggacata tttgatacca ccgacgccag ttcagcatca cggttgtgtg catcgcggcg
    27961 ggcaatacga tacgccagac ggttatcacg cccctgatgg tattgctcca gtatggcatc
    28021 gagataccgg cagttggcct cggtggcgcg ttcgagcatt cgcggcagat tgcgaaactt
    28081 ccagtcaggc cagatgtagc tcactgccgc ccacgcaatg gcacaaccaa tcagcgtatc
    28141 gattacgcga ggtaacgcca cttcaaaacc ttcacccagc aagttgaaac acagtagcac
    28201 caaaagtgtg atgaacatcg ttgcatgggc gtattgcacg ttacggaagg caaaaaagag
    28261 cacgccggta ataaccagca gcaccagctg cccttccagt gatggcacaa accacagcac
    28321 aggaatacca atcgcgatac ctaccagcgt accaataatc cttaacttca ggcggtggcg
    28381 cgtggcgtta tagtttggct ggcagacaaa caaacttgtc agcaagatcc aatacccgtg
    28441 atgcattccg gttatctgaa tgatggcgta gccgaagcac aacaccagcg acattcttac
    28501 cgcatgacgg aagagggcgg attccggcgt gaagtgacgg ctaagacgca gccagatatc
    28561 actcaatccg tgcgggctgt catcagcgag ctcattttcg tcattattat ggggcagtgc
    28621 ctgggcctgt tctgattcaa ttgttgccag ttgggcatca atggcacgta aattgttcag
    28681 taaaaatcct agtgttttga gtaaatcagc aggagcgccg ttatcgcgca tccgctccag
    28741 cgcagcatca atatgcgtaa aagcgcgctc aaaatgcgga tcatgttgat aaggctgacg
    28801 cagcaaaata cagcgtgaca gttgctggca cgcctggccc tgcatcgaca tcagccgctg
    28861 aaaacggaac agcacgtcgc tgtggcgaaa atgttcacgc aatgtttgat attgaatatg
    28921 agaagagctg gcacgctcgt gaatatcctg cgcgacaaag taataatgca gtgtgcgacg
    28981 cgttcctcgt tgaccacgat cgccacgtaa gcgggtcagc agcgagagtt tcgtctgatt
    29041 caatgtcgcc atcagctgac cgttggcgag agccaaatcg tacagcggtg cctggctttc
    29101 atcttcaata tcaggatcaa acatgcgcga cttgagctca agataacgcg ccagttgttc
    29161 atagcaacgc gccaggttgt cctgcagcgg gcggaccggg aacagcagat gaccaataag
    29221 tgtcaggacg ttgtaccaga cggcaccggc cagcagatac atcggctgct gataccagtg
    29281 ctcatacagt gacgtcccca acatagtgta aatggcgatc agtaacgcac cgaaggcaat
    29341 tgttgcatag cgttgaccca gaccgccgag caaaatgaag ccgctggtag agagcgttaa
    29401 accaattgca aatagccagg gccagggaaa cagcaattct actgaggccg aggcgataaa
    29461 aaagcagaac agcgtaatga tgaggttacg caaacgtccc gccagtcggt catcgagatc
    29521 ggtcagcgcc gctgccacca tccccagcgt tagcggaatc gtcagtttta catcacccag
    29581 ccaccacgga aacgctgtgg ttccacaaag cgcaataaaa atacgcgcgt aatacagcca
    29641 ggcgctgttc caggtatagc gtttgagcaa aggacttagc ataaaggcca tattagtgga
    29701 ttattcaaaa cgacgacgtg cattggcttc acgcgcagct tgcgctgttt ctaccgatac
    29761 cacgcgacga ccgaccggcc atagtgcaat agcggcaatt ttaaagttcg caatgccgac
    29821 aggaatgcca atgatggaaa tacattgtgc gatgcccgtt gcaatgtgca tcaggcataa
    29881 ccaccagcca aagaaaatca gccagaaaat attcaatacc gtaccgccag tatttagcag
    29941 cacatttttg ccagccgggt tcagttcatc gacatgaata gcttcattgc cataaggcac
    30001 cagagacagt ttagtgatct cccagcagga tcgtgtcagc ggtaaggtaa aaatcagcac
    30061 aatactgacc agagtcgcca acagccagcc cagagtggtg gcaaatccgc caagcacaaa
    30121 gttcagaatg ttcaaaacgg tacgcataaa acctcgcttt actgtggttt tcagtaatgg
    30181 gcggcaattg taacgttttt ttgggctgga gcacgttttc tctgacggtt acactgataa
    30241 gaaataattt cgtgtggatc tacagagtca tggaactgaa agcgacaacg cttggaaaac
    30301 gtctggcaca gcacccttac gatcgggcgg tgatcctcaa tgccgggatt aaagtctccg
    30361 gcgatcgcca cgaatacctt attcctttca atcaattact ggcgattcac tgtaagcgcg
    30421 gtctggtatg gggcgagctg gaatttgtac tgccgggcga aaaagtggtg cgtctgcacg
    30481 gtaccgaatg gggcgagacg cagcgttttt accatcatct tgatgctcac tggcagcggt
    30541 ggagtagcga gatgagcgaa attgcatctg gtgttttacg ccagcaactg gatttgattg
    30601 ccacgcgtac tggagaaaat aaatggctga cgcgtgagca aacctctggc gttcagcaac
    30661 aaatccgcca ggctttgtcg gcgttgccgt tgccggttaa ccgactggaa gaattcgata
    30721 actgccgtga ggcatggcgt aaatgtcagg cctggttgaa agatattgaa agcgctcggt
    30781 tgcagcataa ccaggcgtat accgaagcca tgcttaccga gtatgcggat tttttccgcc
    30841 aggtcgagtc ttcaccgctg aatccggcgc aggcccgggc agtcgttaat ggcgagcatt
    30901 ctctgttagt gctggctggt gcaggaagcg gaaaaacgtc ggtgctggtg gcccgtgcag
    30961 gctggttgct ggcgcgtggt gaagcgtccc ctgagcaaat tttattgctg gcgtttggtc
    31021 gcaaagccgc tgaagagatg gacgagcgta ttcgagaacg actacatacc gaagacatta
    31081 ccgcacgcac atttcatgcg ctggcgctgc atattattca gcagggcagc aaaaaagttc
    31141 cgatagtcag caaactggaa aatgataccg ctgcccgtca tgaacttttt attgctgagt
    31201 ggcgcaagca atgcagcgaa aagaaagcgc aggcgaaggg ctggcggcaa tggctgacgg
    31261 aagaaatgca gtggtcagtg ccagaaggta atttctggga tgatgaaaaa ttacagcgtc
    31321 gtcttgcctc tcgcctcgat cgctgggtaa gtctgatgcg gatgcacggt ggtgcacagg
    31381 cagaaatgat tgccagtgca cccgaagtga ttcgcgatct gttcagtaaa cgtatcaagt
    31441 tgatggcccc gttattaaaa gcctggaaag gtgcgctgaa ggcagaaaac gctgtcgatt
    31501 tttcgggcct tattcaccag gcgattgtga ttctggagaa aggtcgcttt atcagcccgt
    31561 ggaagcatat tctggttgat gaatttcagg atatctcgcc gcagcgcgca gcgttgttag
    31621 cggcattacg caagcaaaac agtcagacga cgttgttcgc cgttggtgat gactggcagg
    31681 cgatttatcg cttcagcggt gcgcaaatgt cgctcaccac cgctttccat gaaaactttg
    31741 gtgaaggtga tcgctgcgat ttagacacga cttaccgttt taacagtcgt atcggtgagg
    31801 tggcaaaccg gtttattcag cagaacccag gccagctgaa aaagccgcta aacagcttaa
    31861 ccaatggaga caaaaaagcc gtcacgttat tggatgagag ccatcttgac gctttgctgg
    31921 ataagctctc tggttatgcc aaaccggaag agcgcattct gatcctggcg cgttaccatc
    31981 acatgaggcc tgccagcctg gaaaaagcgg caacacgctg gccgaagttg caaatcgact
    32041 ttatgaccat tcatgccagc aaagggcaac aggcggatta cgtcatcatc gttggcttgc
    32101 aggaggggag tgatggtttt ccggctgcgg cgcgggagtc gattatggaa gaggcgctac
    32161 tgccatcggt tgaggatttc ccggacgctg aagaacggcg gttaatgtac gtggcgctga
    32221 cccgggcacg ccatcgggta tgggcactgt ttaacaaaga gaatccctct ccatttgtcg
    32281 aaatactgaa aaatcttgat gtgccggtgg cgagaaaacc gtaagaaaca ggtggcgttt
    32341 gccacctgtg caatattact tcagacggtc cgcgagataa cgctgataat cggggatcag
    32401 aatatcgacc gcgtcgttga aatgcggcga ctggataatg aagtctgccg ttgccacgtt
    32461 ggtggcgacc ggaatgttcc ataccgtcgc cagacgcagc aaggctttca cgtcagggtc
    32521 gtgcggcacg gcgtttagtg ggtcccagaa gaaaatcaat acatcaattt tcccttctga
    32581 gatcaatgcg ccaacctgct ggtcaccccc cattgggcca ctcaacatcg cgttgacgtt
    32641 catgccggtt gcacgggaaa ttaagttacc ggtagtgcct gttgcataca gtacgtgttg
    32701 ttccagtaac ggttgatgcc gttccaccca gctcatcagc atttgtttgc agtgatcgtg
    32761 tgccaccagc gcaatatgtt tccgcgcagg taaagtgcgt gtcgtcagtt ccataatgta
    32821 catccgtagt taactttcct acagattact gtaagcactt atcgctgcaa gataaagacc
    32881 gaaaaagcct gcgcacaggc acaaaaatct caggaagatg gttgtttttc cgcccattgc
    32941 aggaaagtat ttcgcgtttg tgggtcagcc agtttaaacc aatacttcag ccgttgttct
    33001 gtgagcacct gagactgcgg tggaatagca tccagctcgg aaacacccga aagcagcgta
    33061 ctatcatctg ccatcatcgt ggcaaattgc ggtagtgaag cgcgtttcgc ggatttgtta
    33121 tagcgttcaa cctcgacctc ataatcaatc gtttttgccg ttgaggtaat ggcgagaata
    33181 tccagcttta ccggaatcgg cgtcgcatcg ccatccaaca attcaaggcg cggcgcggca
    33241 tcaaaatggt tagcttcccg ctcattctcc agacgaggca ggcgaaaatt gacctggttg
    33301 atgagctggg tattaaaact gaccaccagc ggtggggaga tatagagccg ttcttcacta
    33361 ttggaaagat gaatcgtctt ctcaacgcga aacactaact gatgcggtcc gttatccagt
    33421 tcaatgctgt ccgcaccgcg cagcagagag ctggaaacct tcttgccgtc gagcaccaga
    33481 agatcgacat cggttgaaag ccgcagcgtg gtggcaaaaa cggataccgg caaacataat
    33541 gcaatcaagg tggtcacgat gccggttttc ataggtagct cctgtcaaaa gaccgcccga
    33601 ataataatgt gtcaaaattt ttctttaatc atacttacta acatatagac atatttcccg
    33661 cttttgctct cattcattcg tattagctgc atggttggca tgtcggcttt gtcgtacact
    33721 ttgttaaaca gtctggagga aataatgaaa gaaaccgata ttgcaggcat tttaacgtcc
    33781 actcacacca ttgcgctggt gggggcaagc gacaaacccg atcgtcccag ctatcgcgtg
    33841 atgaaatatc tactcgacca ggggtatcac gtcattccgg tttcgccaaa agttgctggc
    33901 aaaacactgc tgggccagca ggggtatggc acgctggcag aagttcctga aaaagtcgat
    33961 atggtcgatg ttttccggaa ttcagaagcg gcgtggggcg tagcgcagga agcgatcgcc
    34021 atcggcgcga aaaccctctg gatgcaactg ggggtgatta atgagcaagc ggcagtgctg
    34081 gctcgggatg ccggattaaa tgtagtgatg gatcgctgcc cggctattga gatccctcgc
    34141 ctgggcctgg ccaaataaaa aatccccgga aggcaaaaac cttccgggga tttgttcagg
    34201 gattagttac gcagacgcgg ggcctggagt tgtttacgga tggtctgcgc cagctcatcc
    34261 atggatggct gttcaggatg ttcatcctgc aattcgctac ttagctgggc ttcagccagg
    34321 taggtatgaa ccggtaggcc gttatcgtcc tccatcacca cgtgatacca cggagcggcg
    34381 cgaagctcgt cattcaccgc cagctcatca ggcgacggtt cagagagcga ataaaccggg
    34441 tcgatatcca cgaccactcc gagataacct aacagggaat ggcggacctg ctggccgata
    34501 ccgaatttgc tggcaatcat agtcacctcc cgggaaatct ttacctttga tataagggta
    34561 aaattccaca tttcaagtta catgacgcga caggcaaacc ctttcagata tagcccttcc
    34621 ggataggtag cgatcaccgg atgatcggct gcctgacgga actgctctat aaattgtaca
    34681 tcacgaccgg catcaattgc ggcatccgcg atgattttct gaaataaatc gctggtcatc
    34741 agaccggaac aggagaaagt caggagaata ccgccttcat tcagcagctg aatcgccagc
    34801 atgttgatgt ctttataacc acgacacgcg cccatcaact ggcttttatt ctcaacaaac
    34861 ttcggcgggt ccatcacgat aacgtcaaat ttttcaccgc gatcgcgata agtacgcagc
    34921 aatttaaaga catcatcacg gacaaactca gccttgctca gatccagttt gttcagctca
    34981 acgttctgcc gtgcaatatc cagggcttcc tgggaggtat caacgctgac aacctggctg
    35041 caaccgccca tcagtgccga tacggcgaaa ccaccggtat aggagaaaca gttaagtacg
    35101 cgtttatttt cgacgtagcg gcgggtagcc aggcggctgt cacgctgatc caggtagtag
    35161 cccgttttgt gtccgtgctg aatattcacc agcagtttca tgccgtgttc ttcaatcggc
    35221 agcagggcag gcggcaactc gccggtgatg gggccctgag tcagctccat cccttctttt
    35281 ttacgtaccg cgacgtcgct gcgatcgtaa atggcacatt ccgggtacag cgtttgcagg
    35341 gcgctaatta atgccgcgcg ctgatactct gcgccagcac tcagcagttg cagcaccaga
    35401 aaattaccga aacgatcgat agtaataccc ggcaggccat cagattctcc ggcgattaaa
    35461 cgatagctgt cgaggccatc tttttgcgcc agccagtcac gccatttttg tgcttgctgc
    35521 aaacggcggg taaaaaaagc aatgtcgata gattcattcg ggtcaaacgt ccagacgcgc
    35581 gcccggattt gcgaagctgg cgaataagcg ccgcgtgcta accattttcc ctgatgatca
    35641 acaatatcga tggtttcacc gaggctggct ttaccttcca tgcgggcaac ggccccggaa
    35701 aagacccacg gatggcgacg aagtaatgat ttttcgcgcc ctttggctaa cactaaacgt
    35761 acactcataa tttacttttc tgtcgatgca aagaaatggg cgtcattgtc cggagttttc
    35821 catggatttg caacgcgtcc attaaggata aggagagcag caatgtcgaa agtctgcata
    35881 attgcctggg tttacggtcg agttcagggc gttggatttc gctataccac gcagtacgaa
    35941 gcgaaaaaac tgggattagc cgggtacgcc aaaaatcttg acgatggcag cgtggaagtg
    36001 gttgcctgcg gtgaagaagg gcaggtagaa aaattaatgc agtggttaaa gagtggcggc
    36061 ccgcgttctg cgcgtgtaga acgggtgctc agcgagccgc atcatccctc gggggaatta
    36121 accgattttc gtatccatta aatacatttt accggttttg gcaggccagc aattttggtg
    36181 gcttgcttag ccggaccttt cggaaacagt cggtataaat agcggctgtt tcctttttct
    36241 tcgccaaatt tattcgccat cgcttttacc agcatacgaa tcgccggaga agtattgaat
    36301 tccagataga aattacgcac aaaacgcacc acttcccagt gttctggcga cagcgtaatc
    36361 ccttcgttct ctgcaatcac cactgccagc ggctcactcc actggctgct ttctttgaga
    36421 tagccttcgg tatccgtttc tatctcttta ccttcgaaga tcagcataat tattactacc
    36481 ttaatcagac tggcggcagt gtaacaaaaa acaaagcccc gcataaagcg aggctatgaa
    36541 agtgttagcg ggtaagatta atcgcggctg gcgaagccca gaatgctcag caggctgacg
    36601 aagatgttgt acagcgaaac atacaggcta accgtggcac gaatatagtt cgtttcaccg
    36661 ccatgaatga tgttgctggt ttcaaacaag atagcgccag aggagatcag aatgaagacc
    36721 gcgctgatcg ccagatgcag agcaggcagt tgcaggaaga tattagcaac cataccaatc
    36781 agcaccacca caatacccgc catcagcata ccgccgagga acgacatatc tttgcgggtg
    36841 gtcagcacat atgcggagca gcagaagaac actaacgccg ttccgcccag tgccatagcg
    36901 attacgtcac ccattccggc agacagatag gtattcagaa ttggtccgag gatataaccc
    36961 agaaaaccgg taaaggcgaa tgcggagata atcccggtcg gcttattcgc cgttttatag
    37021 gtcaggaaca tcaaaccata catacccacc agcgtcagaa tcagacccgg agatggcagc
    37081 atcagcaccg tactggcagt tgcggtaatc gccgaaaagg ccagcgtcag gctcagcaga
    37141 aaataggtat tacgcagcac cttatgggtg ctaagcagcg atgtacggtc atgtgaagaa
    37201 ctaacaatac gatccattga gtcactctct atgacagatg taattaatta agcagcataa
    37261 tgataatgcg taagggcacc cagaagtttt acccatcttt acgcatttga tctggaacag
    37321 gtttaacagc ggattatcag gttattaagc aatgatataa cgctctgaga atttcgacag
    37381 acaaaagaaa aaggggttag catttagcta accccttatc ttatttggcg gaagcgcaga
    37441 gattcgaact ctggaaccct ttcgggtcgc cggttttcaa gaccgaagaa aaacaaataa
    37501 aaatcagtgt aatacgtgaa gctgctgaaa atttaatcaa atttaacacc aagaaaaatc
    37561 aaggtgttag gaagttgctc atagctagta taccaagtat ttttgccaga aaattttctg
    37621 tattgcctac ttgctaacca cgagcggcag atccagtgtt ggtgtgattt ttgttttacg
    37681 atcgtaaatc aacacctgat tttctgtttt atgcccgctg aaaatttgtt tgtcgcgact
    37741 gctgccttcg taatcagaga tccccttggc ttttatatcg tgaaaattgc acccgaacgg
    37801 aacgccaact ttttgctcgg ctgagcgttt agcctgattc caccagttgt tcagcgtttt
    37861 agctatgact ttcccgcctt tggttgtatt gatcacatat tcgcatgtac aggaagatac
    37921 atttcgggct aactggatcg ctgtacgtaa tcgcggtgac cattccttga tttgtttggt
    37981 gccggttttg ttttgctcaa tgtagatccc tttatccata atatcctgcc atttcaactc
    38041 aagcacatca ccgagccttg ccgcacagag ataggaaatc tccattgcaa tgcgtaactg
    38101 tggaattgct tccgcatata tcgctgcata ctgttcatcg gtgatgtaaa cagtgcgggc
    38161 tttaagagag aattttctga ctcctttgca tgggttattc ttcacgtatc cacgctcata
    38221 tccccacccg tatactcgac tcaggcttgc cagttcatga tttgcctggg tcttgctctc
    38281 aagccctcgt ttatccatga aaattcttac ctgctcagtt ttgacattat cagcaagcac
    38341 ttttccgaat accatcagca acgccttctg atgttgtcga taatcttttt gggttcgggg
    38401 ggccagttct gtaaatgcag gggagtccat aaacatgtgc cataatttag ctacggtcat
    38461 tatgttgtgg agttttgctt tttccagttc ataattttgc cagactttag ctacgctggt
    38521 tttccgcact cttcctagcc ctatagttct tgtacttcct tctggtttcc acacgtaact
    38581 gtaaccattc gatctaaccc gcggtggcag tacattatct tttttatttt ttcttggtct
    38641 tcccattgtt cagcgcctca aaatcgggtt cagcagaaac cagttcagat gtttttggca
    38701 tcgttgtcag tccgtgtgga atatccctgc gtagaactat tggttcgttt ttaggaccga
    38761 ttacaaatgg gatgccgtgc agtcttaact ggtgttgctg ttttgtgtat cgctcgtatt
    38821 tcgtgatctc tttaatctct gctggagata gagttaattc gtacatgtgg tcacgtgcct
    38881 ttacagcatg accgccgcca ctatagctgg tgggcggcga tatgggttga acattaaaaa
    38941 tcagcctgac tcgggatcag tttttgccag atagctgaaa cgtattttgc ctgataacgg
    39001 gcgtcatcaa gtgcattatg gcgctcacct tcgaatggaa tagccgttct ggcatcgaag
    39061 tctatggctt tccccagctc aacgattgtg cgtacatcgc gatcgttgta gtaacgccac
    39121 gggcagggga tcccctgccg ttcgtatgaa cggcgcaaaa tcgtgttgtc gaagttggct
    39181 ccatttcccc agacctgaac aaaaaattca ccggagtttt cgtcgataaa ttcccgcagt
    39241 tgtaacagtg catcatctaa cgggatttca tcggtcataa tggcagattg cgcttcgcgt
    39301 gattgcttaa gccaccattt aatggtgtcc cgatcaatga ctccgccagc agtttccaga
    39361 tcgatagtct tactaaattc cggtcccata tctccggttt gcggatcgaa aaatattgca
    39421 cctattgaga tgatcggggc attaggattt tttcccatgg tttcaaggtc gatcattaga
    39481 tggtcacacg tcctgctggt ggatgtgatt tcgtgatgac cgttcaccgc aattaaggga
    39541 tctgccgctt cgccagtttc actatcgctg gtgtgatgct gatcgttacc agcattctca
    39601 ttgttcggat gttcagcgcc ttctatttcc tccggatcat cttcctgaac ttcagcctga
    39661 tttttttcat cgaatgtttc ctggtatgtt gcgtcgccca tcaccgcgcc acagtcagga
    39721 cagttgccgc caccgctctg accgcaggcg gtgcagactt tttcctcttc ctgttgcgct
    39781 actggttcgg attgtttcgt ttctggctcg tcttgtaacg cattttggct gttttgttct
    39841 gctttctggt cgttctgttc cgtttctagc tggttctggt tcacagaatc gcaggtttca
    39901 atccccttaa cccatttcga atcattcggg tcgctaatcc cttcaacaaa ctcaccacga
    39961 gaagcggcaa gcaatttgtc ggcatctggt cgactgatgt ctgctgcctc cataatttta
    40021 tttacttctt cagcggtaac ttttacctgt gacttgtcat ccagcggctg cgggtcctga
    40081 tgatgttcaa ctgtatccgc tgccattgtt tcagccgttg cctgttcatc tgccattgcg
    40141 ccagatggtt gcgggttttc ttcatcattt ttttcttctt ctgttttgca ctcagcagcc
    40201 agtccgtcgt taatttcttc caggatatct ttttccggtg tatgccgggc agccatgagc
    40261 gtttcagctg tgggattctc gtgatcagtt tctgtcaggt tggcattgat atacccctga
    40321 aggcgtcccg ggtagtgata aaactcaggg tgggcgctcc ggataagtgc aaaaatagct
    40381 gcgcgggaat agtccaggat accaggggtt gcgcgaagtg ctgcggacca ttccctgaac
    40441 ggactttctt ttttcaggac gatttctttt gcgcgacgat aaacgctcac gggtatttca
    40501 taaatattaa aatccatcgg aagcgtggct gctgcagcct ccacatccag agtatcgagg
    40561 gtgtgtacta agtcaggatt acgatcggtt ttatttccgc caccggcatt tgcaccggaa
    40621 gccgtgcggg tgatgcgaga aacacgattt cctttcatcc actcttttgt cagcagacct
    40681 cgatcggtat agtcagcgtc caggtatgct tcgaaaaaag cagtcattaa ccccagactg
    40741 gaattaacgg gattagggaa aactttgtct gtgtcgcgta ccagtttatg aaggtcgcga
    40801 atctccagcg ggtcgagcag ctttgtgttg tgggaaatag ccagggcagt aacagccggc
    40861 agttcttcag ctcgtgcgat atgtaatgcc tgaagttctt cccgtgaaac gtgcgttacc
    40921 ggtttttcgc tgccatgttg cgcaagccag cgaatgggta gttcctgacc ggaaattggc
    40981 aggagcatat tatcctcaat ctcagccatg tcttcgccat tgacgttggt attgtcagtg
    41041 ctggctggtt tttcctgaac tgagggggaa ggcgctataa atatcattgt gatgccatct
    41101 tccccgcctt tttcatagcg gttgcagaat tcagtatcaa acacgccttc cggtggaagg
    41161 tcattcacaa cggggaaatg gacgcggacg ggttttttga aatcatcagc gtcgtagcct
    41221 gcctcgtcca ttgcaacaac gcaccgcgag attgctacag ataatttctt ttctgtactc
    41281 cagtaaaaac cccctttaat cccaaagcgt tttctgacct tgtcattttt tgcttcgcaa
    41341 tatagcgcaa attcttcttt atcagtgctc attgataaac ctcataacta ttttaaggtt
    41401 gtacgaaccc ctgccattgc tggcatgctt aatcaacggg tatggcgtta atatggctgg
    41461 tggattatcc agccggtatt tcgttattca ggtacagcga tacttttttt aacgggaggc
    41521 attcgccaga aattttttgc tcgtctcttg cccggaggca tgattcttta ctggcataaa
    41581 ttccggtaat cacattctgt ggctcacccg ttataagaaa aacagtcatt accagtgcaa
    41641 atgcagaagt cactgctgcc ccccgaaaat accaagttta agaagggcaa ttctggagag
    41701 tatggaatta tcattgagaa gataaggctc atattttctc atcttaatgg catcttcagt
    41761 aaactcccgg ttactgagca gaacaccaat atcaaaacac ccttcagacg tattaacgtt
    41821 tggtaataac gtttccatta tcgtgtcctc aacaatgaat tttgtgatgt ggtgcctggt
    41881 gcctccaggt gacgttaacc agttaacaat taacgccgga atacagaagg atgcccgtta
    41941 cgccccgtaa aagaccactt tacggattta actgttccgc gtgcgcatag ccgcattcac
    42001 cgcatcacaa aattcacttt aaaaaggatg gcagagcagt catggagtcg aactgatgcc
    42061 accaaagact acacatggat attgtggcgg ggctgtcact taagcgtatg gtcagcctga
    42121 caacccggtg tcctcaaggg gaaggaataa ccccgccata cttaccgccg cgccatttcg
    42181 cggattgcca caaccggaag cgcacggtcg aattaaattt aacgacaaag tacagagaga
    42241 cgagcttctc cgtgcgcttt cgtgttatgc cctgactttt cagggtaaat taaccagtgg
    42301 caacctgttt ttactggtgc taatcagtta gtgtttctgg ctaaccagcg acgcgcgcca
    42361 gcttcggttt taaacttttt acttttggta tacgtcatcg cggtaaacgt gccgtcctgg
    42421 ttagggaaca caccgcatac cagagattcg ttgttgccaa gattgagcgt atccatgttg
    42481 acctcatttc cccttaacgc cggggtagcg gaacaaaaaa cctgctgcat agttaaacgt
    42541 aatccccgcc gtcatgttca tacgcctcag gctggctact taacccctga ccactgccgg
    42601 gtaactcgaa gtattgccct gcgttctgtg ggtcggggtg ggtgtatgga tacaatttac
    42661 aaattaaaat ttaactggtc aacatgttta ttattaaatt tgtaattgcg agctgttgca
    42721 ttagtcccaa aatgggacta ctatacagtt atgaagatta tctcagttaa aacactcagg
    42781 gatttttggg cggagaatcc tgatgcagaa caaccgctaa gggcatgggt ggatgaggcg
    42841 gcaaaagctg actggaaaag cccagcagac attaaggcac aataccgaac ggccagtata
    42901 ttgaaaaacc ggcgcgtggt attcaacgta aaaggtaatc attaccgttt aattgttgca
    42961 attgcgtatc agcgaggatg ggtatttgtt aaattcatcg gaaaccacaa ggaatacgat
    43021 gctattgatg ctgataccat cgaactggag taagcatgaa catcaaacct attcgtacag
    43081 agcaagatta tgaagccgcg ttgcgtgctg ttgaacccat gttcgacaat gagcccgaaa
    43141 ttgacacgcc tgagggggat ttttttgagg tgatgtgttt gctaatagag gaatatgaaa
    43201 aaaaacatta tcccattgag ccaccatccc caattgaagc tataagattt cgcatggaac
    43261 agcaggggct gactgtgaaa gatttggaac ccgcaattgg gaaaaaaaat cgggtttatg
    43321 aggtgttgaa tggtaccaga agccttacgt taccaatgat tcgccgtctt cataatcaat
    43381 ttggtatccc cctggaaagc ctggttggat tataaaatct gctagtcatt tgcctgatgc
    43441 tcgttccaga aaaggaatgc atcaggcagt tttgtttttc tgccgcagta actcttcaag
    43501 tttccgttta tagaaatcgc gtttttgctc catatcacga atgatctgct ctgcgtcgct
    43561 ttgaggtaac tcatctaaaa gcgatatgat ttttcgttgt tgttctgtaa gttgcggttg
    43621 gttgtcatta ctggatacag ccattttatc gccgagagtt tcttcttcca taaagaacca
    43681 atggacggga tgttgtgaga gctctgctaa tttttccagt ttatccattc ttggcatcac
    43741 gcctttcaac caaccttgca cggattgggg ttttacacca agacgtcttc ccagctctga
    43801 ctggtttata ttcaattcct gcaacacctg ctgaaggcgt tttacaaaga tcatcaccac
    43861 ccctcgtaaa ctagttccgc gatcctacag aaaaaattga taagtggcat tacaaataga
    43921 agttgaaatt taaatttaaa tttgtaatca tcggtgtcat cgtaaagttc ggagggaaac
    43981 atgcaaaaaa gtactcaagt gaaaatcctg tcaataatga gccaatcaga attagggcgt
    44041 cgtcttggta aaacaccgca aaccataagt gggtggttta aaaaacgagt gccagcggag
    44101 gaggttatac cagcatgtga ggcgcttgac tggggagtaa ctccgcatga attgcgccct
    44161 gataaatacc ctaatccaac cgatggttta cctgttgagt atcaggctaa cgcacaagca
    44221 gcggcgggag ttgattcatg aaaatcaagc atgagcacat ccgcatggcg atgaatgcgt
    44281 ggctgcttta tccgagggta gggcgcaaaa aaatcgctga tgatatagcg acagcatatt
    44341 ttgagcttga aatgacttat ccaccaatgc atgacacctc tacgacagag ggtattggat
    44401 tgaatataca aaatattttt cgctggcttg aaaaggatac gcctgatgct gttgaaaaaa
    44461 ttcaggcact aattccggct atcctgactg ttcttccgcg tgaactgcgt tatcacctca
    44521 gtatttttga cactgttgag cgccgtgcat tactggcggc gcaggaagcg ttgagcacgg
    44581 caattgatgc acatgatgat gctgtccagg ccgtttaccg gaaagcgtat ttcagcgacg
    44641 gcgggtcatc cggcgagtct gttgtggtgc attgatattt atgccagccc cctgctgatt
    44701 ctgttgattg gggaatcaca gaatatcacc agaggatggt tcgtcacaag atgaggcaat
    44761 tatggccgca ttaccataca tgcaactgta catagctgat tacctggctg acaccatgca
    44821 tttgtccgca gaggagcacg gtgcgtattt gttgctgatg ttcaattact ggcaaacagg
    44881 gaaaccaata cctaaaaaca ggctggcaaa aatttcccgt ctgactaacg agcgatgggc
    44941 tgatgtggaa ccatccttgc gggagttttt ttgcgataac ggcgatgaat gggtgcatct
    45001 tcggattgag gaagatctgg catcagtcag ggaaaaattg accaaaaaat cagccgccgg
    45061 aaaagcatct gttcaggcca gaagaagcag aaaggaagta tatgttcaaa caaaacaaga
    45121 gagagattta acaggtgttc aaacaaatgt tggtgttgtg tttgaacatg acgcaaacac
    45181 aaaagcaact aataaagata cagatctaaa agaattaaac cccacacata acgtgcgtga
    45241 acgcgagagt attccgacca gtgagttgca tggtgcgccg ttgcagacag ccgaacctga
    45301 atacctggac ggcctgagcg aaccgatcgg gaaattttcg atgaccactg tctggcagcc
    45361 gtcgccggat tttcgacaac gggcagcagt gtggggtatg gctctgcctg agccggaatt
    45421 tacacctgct gagcttgccg cattccggga ttactggatg gcggagggga aggttttcac
    45481 acaggttcag tgggagcaga aatttgcccg ccacgtgcag cacgtcaggg cacaggtaaa
    45541 accagtcagc aagggggtaa gccatgcagc atcaggtggc acggcatcac gggcaattca
    45601 ggaaatccgg gcagcacgcg aacagtggga acgagacaac ggatttatca gcaacggaaa
    45661 cggcctggaa gctgtgggag cttatggggg aggtgtattc gaaccgctgg actcagaaga
    45721 acggggccgc accttcgaag ctctggattg cccagattgg cgcgatgact gaacagcaaa
    45781 tccggctggt ctgccgtcag tgcatggacc gctgccgggc gggtgaaacg tggcccccgg
    45841 acctggctga gtttgttgcg ctgatttcgg ggagtggggc aaatccattt ggtcttacgg
    45901 tggatgccgt gatggaagag taccggcgct ggcgcaatga atcctggcga tacgacggga
    45961 gtgataaata cccgtggcca cagcctgtgc tgtaccacat ctgcctcgaa atgcgtacca
    46021 gagggattga gcgccagatg acgcagggtg agttaaaacg acttgcggaa cggcaactga
    46081 cgaaatgggc aaagcatgtt ggtaacggga tgagtgttcc gccagtgcga cgacaactgg
    46141 aaggggcgaa acacccgcaa gggccaacgc caattgaacg gctgaaacag gaatacgaac
    46201 gccggaaggc agctggtttt atttgaatct gagaaacgat tttgtcggag gaaattttaa
    46261 tggaaaccgt atttgacgca ctgaaagcac tgaaaaaagc ctcttcacag gtagtggcat
    46321 cgcgccttgg aatcagccgc gaagatgcgg tcaacgaact gtggaaactg aagcgccgcg
    46381 gtgaagcgga taacaagggt tcgatgtggt ggctgattca ggctggtgaa agtgaaccag
    46441 tgtcaccggt accgaaagtg acagcgcaaa tgctgattaa ggcgattgaa caacatggcc
    46501 cacaaacggc ggatgagctg gcactgatgt tcgggattac ctcccgccgg gcgaattcat
    46561 cgctggccat ggcaatcagc aaagggcgtc tgattcgcgt gaatcagggc ggtaaatttc
    46621 gttactgcat accggtcgct gatttaccgg cagagccgga agctgcatcc gtagcggaaa
    46681 cggatggtaa agcctttcct cagccagcag gtgttgcgtt accagtccgg gaagcggaaa
    46741 cacaggaaga tattaaaaca gaaactgtgg ctgatattgt gcaatcgttg ccatcgttta
    46801 ctgaaacgcg agcggatgac ctggttttac catcgctgca tgtggctaac cgcgagctgc
    46861 gccgggcaaa aggtcaggtt cagaagtggg agcgagtctg tgccgcgctg cgggaactga
    46921 acaaacacag ggatattctc cgggatatta ccgccaccag agagctgctg cggtgagtgg
    46981 ctggaagaag tggcgctggg cggaaattat gatactccgg cagtgtgcgg gaacgatgac
    47041 agtcgaaagc atcggcagtc tgattggccg tagtggggct gccgtcagga cgaaagcgcg
    47101 ggaactgggt atctgcatga tgttacgggg tgattatcac cagtcagcca aatgttcaca
    47161 gcgtgatatt gagctggcgc ggcaactgca tcagcgtggc gtaccccgac gggaaattgc
    47221 cgaaaagttt gggatgaagt tgggcgcagt gaataactac gtttatttcg acaggagggt
    47281 tcaggcgtga gggtgagaat ttatatcgcc ggtccgatga cgggatatga aaatttcaac
    47341 cgtgaggcgt ttcacaaggc ggaagaagca ctgaaacagg aagggcatac cgtattaaat
    47401 cccgcagtac ttccggacgg gctgactcag ccacattaca tggatatttg tatggcaatg
    47461 attcgttgtg tggatgcggt ttatatgctg aaaggctggc agcggtcggc aggcgctaag
    47521 gcagaactgg cactggcgga gaaactgggg catgcagtta ttttccagga ggcaaccagt
    47581 gagaaaaatt aattatcaga cgctacgtga gatagcaaaa ctggcaaccc agggcgaatg
    47641 ggtcgcattc atttcgccgg gcactgatac atatgcggtg catacgccgg gggatgaacg
    47701 ttgcggagac attatcaaat ggcctggctt tgatgaccag aaaaatgcag agaacaacgc
    47761 agaatttatt gcagctttca accctgaagt agtacaggca ctgctggatg aacgggaagc
    47821 ccaaagcaaa cgtattgccg agctggaagc taatcttgtg gcgctggctg cggagaatgc
    47881 acttgctcgt aaagcagttc aggcattctg cgatgttgtt ggcgacaaca ccgaggttat
    47941 ttgcgaggag gttgggagag atggcgttct ggttattttg gaggccatga aggcaacagg
    48001 aaatatgcca gccaccgatg ctttcctggc tgatgtacga gcggaggtgt ttaacgacct
    48061 ttgcgcggcg tttgtcaggc acgcaaaagt tgcaggactg gatgatgccg ataccgtaac
    48121 gtttaaggaa gcgacagaag cattgctgca ttgtgcagaa caacttcgcg cacctgaata
    48181 attaaattta gcactgtaaa taaaatttaa tccttaaccg gagggattcc tgcaccctca
    48241 gaacatcagg agaccgcccg gcagggcggt agtgaaatgc gaaagttcaa aataattatt
    48301 gaaacgggaa tagccggtgg agattttgag gatgtattcg aagtggacga tgacgcaaca
    48361 cctgatgaaa ttcatgacga agcaaaagaa attttcttta actactgcaa ttactcatat
    48421 cacgaaataa aagacgaaga ggaagaacaa aatggctgat tttggttcaa ctaaatataa
    48481 cgccagtttt gaagaatggc atgaactgtt aatggactat gcagagttac gcggtggaag
    48541 tgccgccgat gctgaagcat ggcgtgatga ctatgaagca ggaaaaactc cggttgaagc
    48601 ttattgtgat gagtggggtg aggacgatga agattaacta tcaggcgatg gtgcaggcca
    48661 ggcgattcga taccggctcg tgttcagagt catgtgaaat ctggggtgaa caattggaat
    48721 tgaaattcga agaggtggtg gcatgacaat cgtaaaaacc cataccggca tcgtgatcac
    48781 caaagacggt ccgcaggtaa aaaaactgca ccagacggag cggatgtggg tcgtcgacaa
    48841 aaacgagttt taccacaaag aaaccggacg ccgccacttt gcagaaaata cgcgccgccg
    48901 actactgatc gacaccatca agcctgtcga ggtgaagcat gtttaaacag aacgaaaaat
    48961 ctatcgctca aattgctgag tatatcccgc gtgcgtgccg gggtatgcag ttgcaggaag
    49021 ccaaagcgcg cctggagaaa aaaattgcgc tctatatcaa tgacggctgt gatgctgccg
    49081 ttcttaacgc ggcgttcgca ccagctctta acagtcatac gcgggagtct tttttttcgt
    49141 gcatcgcagc gcagatccac aaaggaggca accagtgagc aagattgact atcaggcatt
    49201 gcgtgaggca gcacaaaact atcggtcgat gctggcgtgg tatcaggaga aaccagacag
    49261 tccaaacgct gagcaggatt gtgatgcagc tttgtctgcg tttaagcgag aaatccgtca
    49321 tcgtgaagtg gatattatcg ctgatttgct ggatgaactg gaggaagcaa aacagcgtat
    49381 cgacgagcag gagtcccgca cagtgaagtt accagaacca ttcaagctgg ctaaatcatc
    49441 aagtggatta acgtactact acgctgatga ggtcaatgca gcactgactg ctgctggtat
    49501 tcgcatagaa ggagagtgag atgaccacta ttaccaaaga gcgactgcaa tggctggcta
    49561 acatttctgg ccgcgatgac attgacgata tagacggcgg tgaaattcgt gagctggcgc
    49621 ttatcgcact ggcatcgctg gaagcagaac cggtggttta catgtgggat agcgaaagaa
    49681 aagatattga tgctcctggg tattatcgga ctgagcattt agtgttcgct gagagtagtg
    49741 ttaagcaatg gggagggcga gttgttccac tttacaccgc cccgccagcg ccgatagtgc
    49801 cagctgttct ggaacgtttg cgagctattg tagcggaccc acgcgcatta cctcgcagaa
    49861 aagaatgggt tagcgggcag cagtacagtt acgtacttct cgaaaacgta gaagctatgg
    49921 ttgatgaagc ctgccgcgca gccatgcttc agggagataa ataatgatta atcgaaccaa
    49981 actggagcac attctcgaat atgccaggca gcagaaatgt attgggcaac tttgtaaaat
    50041 tccaccaggg gatatggttg aaatcatgga gattgccatg cgcaaggctg gcaactctcc
    50101 gataactcag gctagtctgc ctggcggttt caccattgag gaggcgaagg agttacatga
    50161 agacctggtg cgcagccaca taagcaaggc tttaagtggc gaaaagatga agaagaagga
    50221 tctcgatgct gatttgtgct ggatccatgg ggttatagtc caggcatcct ggtttgtaaa
    50281 agcatcactg gagcagaatg cgctaacggg taactctcag gtaatccctg atgacggtcg
    50341 cgaacagttt gaaacactta tcaggttcca tgcagatgat aaaaaccatg aaacattatt
    50401 gcttcgtgct aacgaaggga tgaattaccg ggatccaaat gtggatttgg cgtggatatt
    50461 ctggaaatcc agtcgagagc acatattgcc gggaaaaagc gacaataatc cagcattagg
    50521 cgatcaggtg agtgaattaa ctatgttggt caagcgatta gccagttcat taaaaagcgt
    50581 caataaatca agcaaactac cggataaagc gatggactac ctgaagcgaa acggactgat
    50641 aagcgcggag gatattttac gatgacctgg cctgaggcat tcgtggcggt aggaattgca
    50701 atggcggtgg cgctggtgct ggtggtgtat tcgatttgcc gctggggata aaaacggttt
    50761 gcggggaaag aagagttaag tagaagtgct gcgggtgctt gaggctgtct gcctcgggca
    50821 tgccgccgta aggcagacag agaaaagccc cagttaacat tacgcgtctt gcaggacgct
    50881 taacattaat ctgaggccca atctatgctt cacaaacgta ggttagcctc ttacggaccg
    50941 aaaggtcaag gagaagcaga ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt
    51001 catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct gcgaggtacg
    51061 aatccgaacc ggccagacgg aggttgctgt cttcgtagac tacgaatctg agaagtaaga
    51121 gtgaccaggc gagggagaaa tccctcgcca cctctgatgt gtcaggcatc ctcaacgcac
    51181 ctgagataaa atctatttgg ctgtttctgg gttcgaactg atgattattt ggtgctaata
    51241 catgatttaa ttccctgaat cctcttgctt ttcccattgg tggggtctaa gactcctcga
    51301 agggaataaa aatcaatgat agtagaacca tctgccaact tataaatgtc ggcattatct
    51361 ccaaccgcta atacgctgta atacttgcca attttttgag acgtgatagt ggctaagaga
    51421 ggtgtacgct gccactcact gagaatgcat tcttgtattt cttgtggtgg agaatttgtt
    51481 ttaaacgaga taataacat
//
LOCUS       DAZKJR010000015        51365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKJR010000015 DAZKJR010000000
VERSION     DAZKJR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.015"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..756
                     /gene="ydhY"
                     /locus_tag="ACHOWS_000921"
     CDS             130..756
                     /gene="ydhY"
                     /locus_tag="ACHOWS_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFQ0046548.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTHRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            777..2879
                     /gene="ydhV"
                     /locus_tag="ACHOWS_000922"
     CDS             777..2879
                     /gene="ydhV"
                     /locus_tag="ACHOWS_000922"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFQ0046549.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRKTLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2892..3530
                     /gene="ydhW"
                     /locus_tag="ACHOWS_000923"
     CDS             2892..3530
                     /gene="ydhW"
                     /locus_tag="ACHOWS_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFQ0046550.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDHADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3594..4262
                     /gene="ydhX"
                     /locus_tag="ACHOWS_000924"
     CDS             3594..4262
                     /gene="ydhX"
                     /locus_tag="ACHOWS_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFQ0046551.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTMQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4259..5044
                     /gene="phsC"
                     /locus_tag="ACHOWS_000925"
     CDS             4259..5044
                     /gene="phsC"
                     /locus_tag="ACHOWS_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFQ0046552.1"
                     /translation="MNPSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSANDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     TVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5048..5860
                     /gene="ydhT"
                     /locus_tag="ACHOWS_000926"
     CDS             5048..5860
                     /gene="ydhT"
                     /locus_tag="ACHOWS_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFQ0046553.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5866..7476)
                     /gene="ydhS"
                     /locus_tag="ACHOWS_000927"
     CDS             complement(5866..7476)
                     /gene="ydhS"
                     /locus_tag="ACHOWS_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFQ0046554.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEEIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAIAQAVVKPASRARRRLSFNQM"
     gene            complement(7602..7907)
                     /gene="ydhR"
                     /locus_tag="ACHOWS_000928"
     CDS             complement(7602..7907)
                     /gene="ydhR"
                     /locus_tag="ACHOWS_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFQ0046555.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTNEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8015..8091)
                     /locus_tag="ACHOWS_000929"
     tRNA            complement(8015..8091)
                     /locus_tag="ACHOWS_000929"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8055..8057),aa:Val,seq:gac)
     gene            complement(8096..8172)
                     /locus_tag="ACHOWS_000930"
     tRNA            complement(8096..8172)
                     /locus_tag="ACHOWS_000930"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8136..8138),aa:Val,seq:gac)
     gene            8481..9737
                     /gene="ydhQ"
                     /locus_tag="ACHOWS_000931"
     CDS             8481..9737
                     /gene="ydhQ"
                     /locus_tag="ACHOWS_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFQ0046556.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGSINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9778..11151)
                     /gene="mdtK"
                     /locus_tag="ACHOWS_000932"
     CDS             complement(9778..11151)
                     /gene="mdtK"
                     /locus_tag="ACHOWS_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFQ0046557.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11366..12007
                     /gene="ribE"
                     /locus_tag="ACHOWS_000933"
     CDS             11366..12007
                     /gene="ribE"
                     /locus_tag="ACHOWS_000933"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFQ0046558.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12047..13195)
                     /gene="cfa"
                     /locus_tag="ACHOWS_000934"
     CDS             complement(12047..13195)
                     /gene="cfa"
                     /locus_tag="ACHOWS_000934"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFQ0046559.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13486..14697)
                     /gene="punC"
                     /locus_tag="ACHOWS_000935"
     CDS             complement(13486..14697)
                     /gene="punC"
                     /locus_tag="ACHOWS_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFQ0046560.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            14810..15742
                     /gene="punR"
                     /locus_tag="ACHOWS_000936"
     CDS             14810..15742
                     /gene="punR"
                     /locus_tag="ACHOWS_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFQ0046561.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15739..16764)
                     /gene="purR"
                     /locus_tag="ACHOWS_000937"
     CDS             complement(15739..16764)
                     /gene="purR"
                     /locus_tag="ACHOWS_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFQ0046562.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17063..17152
                     /gene="cydH"
                     /locus_tag="ACHOWS_000938"
                     /gene_synonym="ynhF"
     CDS             17063..17152
                     /gene="cydH"
                     /locus_tag="ACHOWS_000938"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFQ0046563.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVVVGLTAALH"
     gene            17318..18487
                     /gene="ydhP"
                     /locus_tag="ACHOWS_000939"
     CDS             17318..18487
                     /gene="ydhP"
                     /locus_tag="ACHOWS_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0046564.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18633..19214)
                     /gene="sodB"
                     /locus_tag="ACHOWS_000940"
     CDS             complement(18633..19214)
                     /gene="sodB"
                     /locus_tag="ACHOWS_000940"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFQ0046565.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19342..20169)
                     /gene="mepH"
                     /locus_tag="ACHOWS_000941"
     CDS             complement(19342..20169)
                     /gene="mepH"
                     /locus_tag="ACHOWS_000941"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFQ0046566.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20504..20851
                     /gene="grxD"
                     /locus_tag="ACHOWS_000942"
     CDS             20504..20851
                     /gene="grxD"
                     /locus_tag="ACHOWS_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFQ0046567.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20902..25518)
                     /gene="lhr"
                     /locus_tag="ACHOWS_000943"
     CDS             complement(20902..25518)
                     /gene="lhr"
                     /locus_tag="ACHOWS_000943"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0046568.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSMERLDTLLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQADEWYSRVRRAAPWKDLPRRVFDVTLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARTLLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEANQANSSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSTNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANAWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25611..26258)
                     /gene="rnt"
                     /locus_tag="ACHOWS_000944"
     CDS             complement(25611..26258)
                     /gene="rnt"
                     /locus_tag="ACHOWS_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFQ0046569.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26361..26768)
                     /gene="gloA"
                     /locus_tag="ACHOWS_000945"
     CDS             complement(26361..26768)
                     /gene="gloA"
                     /locus_tag="ACHOWS_000945"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0046570.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26849..27946)
                     /gene="nemA"
                     /locus_tag="ACHOWS_000946"
     CDS             complement(26849..27946)
                     /gene="nemA"
                     /locus_tag="ACHOWS_000946"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFQ0046571.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(27983..28582)
                     /gene="nemR"
                     /locus_tag="ACHOWS_000947"
     CDS             complement(27983..28582)
                     /gene="nemR"
                     /locus_tag="ACHOWS_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFQ0046572.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATPAV"
     gene            28685..28924
                     /gene="ydhL"
                     /locus_tag="ACHOWS_000948"
     CDS             28685..28924
                     /gene="ydhL"
                     /locus_tag="ACHOWS_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFQ0046573.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            28973..29869
                     /gene="ydhF"
                     /locus_tag="ACHOWS_000949"
     CDS             28973..29869
                     /gene="ydhF"
                     /locus_tag="ACHOWS_000949"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0046574.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDYLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            29950..30471
                     /gene="sodC"
                     /locus_tag="ACHOWS_000950"
     CDS             29950..30471
                     /gene="sodC"
                     /locus_tag="ACHOWS_000950"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFQ0046575.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30472..32484)
                     /gene="ydhK"
                     /locus_tag="ACHOWS_000951"
     CDS             complement(30472..32484)
                     /gene="ydhK"
                     /locus_tag="ACHOWS_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0046576.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELQHL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGSNALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32484..33341)
                     /gene="ydhJ"
                     /locus_tag="ACHOWS_000952"
     CDS             complement(32484..33341)
                     /gene="ydhJ"
                     /locus_tag="ACHOWS_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFQ0046577.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSNLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33344..33580)
                     /gene="ydhI"
                     /locus_tag="ACHOWS_000953"
     CDS             complement(33344..33580)
                     /gene="ydhI"
                     /locus_tag="ACHOWS_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFQ0046578.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLSLAILIAW"
     gene            33781..34215
                     /gene="slyA"
                     /locus_tag="ACHOWS_000954"
     CDS             33781..34215
                     /gene="slyA"
                     /locus_tag="ACHOWS_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFQ0046579.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(34262..34729)
                     /gene="slyB"
                     /locus_tag="ACHOWS_000955"
     CDS             complement(34262..34729)
                     /gene="slyB"
                     /locus_tag="ACHOWS_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFQ0046580.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35003..36112
                     /gene="anmK"
                     /locus_tag="ACHOWS_000956"
     CDS             35003..36112
                     /gene="anmK"
                     /locus_tag="ACHOWS_000956"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFQ0046581.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLILSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36210..36539
                     /gene="mliC"
                     /locus_tag="ACHOWS_000957"
     CDS             36210..36539
                     /gene="mliC"
                     /locus_tag="ACHOWS_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFQ0046582.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLFERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36598..37254
                     /gene="pdxH"
                     /locus_tag="ACHOWS_000958"
     CDS             36598..37254
                     /gene="pdxH"
                     /locus_tag="ACHOWS_000958"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFQ0046583.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37383..38657
                     /gene="tyrS"
                     /locus_tag="ACHOWS_000959"
     CDS             37383..38657
                     /gene="tyrS"
                     /locus_tag="ACHOWS_000959"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0046584.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38716..39579
                     /gene="pdxY"
                     /locus_tag="ACHOWS_000960"
     CDS             38716..39579
                     /gene="pdxY"
                     /locus_tag="ACHOWS_000960"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFQ0046585.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39623..40228)
                     /gene="gstA"
                     /locus_tag="ACHOWS_000961"
     CDS             complement(39623..40228)
                     /gene="gstA"
                     /locus_tag="ACHOWS_000961"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFQ0046586.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40334..41836)
                     /gene="dtpA"
                     /locus_tag="ACHOWS_000962"
     CDS             complement(40334..41836)
                     /gene="dtpA"
                     /locus_tag="ACHOWS_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFQ0046587.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42447..43082)
                     /gene="nth"
                     /locus_tag="ACHOWS_000963"
     CDS             complement(42447..43082)
                     /gene="nth"
                     /locus_tag="ACHOWS_000963"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0046588.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43082..43777)
                     /gene="rsxE"
                     /locus_tag="ACHOWS_000964"
     CDS             complement(43082..43777)
                     /gene="rsxE"
                     /locus_tag="ACHOWS_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFQ0046589.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(43781..44401)
                     /gene="rsxG"
                     /locus_tag="ACHOWS_000965"
     CDS             complement(43781..44401)
                     /gene="rsxG"
                     /locus_tag="ACHOWS_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFQ0046590.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44405..45463)
                     /gene="rsxD"
                     /locus_tag="ACHOWS_000966"
     CDS             complement(44405..45463)
                     /gene="rsxD"
                     /locus_tag="ACHOWS_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFQ0046591.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45464..47686)
                     /gene="rsxC"
                     /locus_tag="ACHOWS_000967"
     CDS             complement(45464..47686)
                     /gene="rsxC"
                     /locus_tag="ACHOWS_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFQ0046592.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKACKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(47679..48257)
                     /gene="rsxB"
                     /locus_tag="ACHOWS_000968"
     CDS             complement(47679..48257)
                     /gene="rsxB"
                     /locus_tag="ACHOWS_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFQ0046593.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISYNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48257..48838)
                     /gene="rsxA"
                     /locus_tag="ACHOWS_000969"
     CDS             complement(48257..48838)
                     /gene="rsxA"
                     /locus_tag="ACHOWS_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFQ0046594.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFFQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48915..49355)
                     /gene="ydgK"
                     /locus_tag="ACHOWS_000970"
     CDS             complement(48915..49355)
                     /gene="ydgK"
                     /locus_tag="ACHOWS_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFQ0046595.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49441..49656)
                     /gene="ydgT"
                     /locus_tag="ACHOWS_000971"
     CDS             complement(49441..49656)
                     /gene="ydgT"
                     /locus_tag="ACHOWS_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFQ0046596.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(49929..50054)
                     /gene="blr"
                     /locus_tag="ACHOWS_000972"
     CDS             complement(49929..50054)
                     /gene="blr"
                     /locus_tag="ACHOWS_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFQ0046597.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50297..51337
                     /gene="ydgJ"
                     /locus_tag="ACHOWS_000973"
     CDS             50297..51337
                     /gene="ydgJ"
                     /locus_tag="ACHOWS_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0046598.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEGWGYDMRD
                     GVLTRVEGEERIEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
BASE COUNT        12913 a        12726 c        13389 g        12337 t
ORIGIN      
        1 tttctcatac actattgggt tagggaaaat atctggtgtt gaggacagag tcagcagcgt
       61 attagttgag tgcctaatat caggctgctg aattgtcagc actgtgtttg cgaagaggat
      121 gcgaaataaa tgaacccggt tgatcgtcca ctattaaata ttggcttaac gcgattagaa
      181 ttcttgcgta tatcaggaaa aggccttgct ggtttaacaa ttgcccctgc gttactgtca
      241 cttttgggtt gcaaacagga agatattgat agcggcacag taggcttgat aaatacaccc
      301 aaaggggtgc tggtgaccca tcgtgcgcga tgcacgggtt gccaccgctg tgaaatctct
      361 tgtaccaact tcaacgatgg ctcagtaggg acattcttct cccgtatcaa aatccatcgc
      421 aattatttct ttggcgataa cggggttggc tctggtggcg gcctgtatgg cgatctcaac
      481 tataccgcgg acacctgccg tcaatgcaaa gagccgcaat gcatgaacgt ctgcccgatt
      541 ggtgcgatta cctggcagca gaaagaaggc tgtattaccg tcgatcataa acgttgtatt
      601 ggctgtagcg cctgtactac ggcatgcccg tggatgatgg ccaccgtaaa taccgaaagt
      661 aaaaaatcct cgaaatgtgt gttatgcggt gaatgcgcaa acgcctgccc aacaggggcg
      721 ttaaaaatta tcgagtggaa agatattact gtgtgaatct tgcaaaggaa aaaatcatgg
      781 ctaacggttg gacaggtaat atattaagag tcaatctcac gacaggaaat attaccctcg
      841 aagattccag taagtttaaa agttttgtcg gtggcatggg cttcggctac aaaattatgt
      901 atgacgaagt accgccaggc acgaaacctt tcgatgaagc gaataaatta gtttttgcta
      961 ccggcccatt aactggatct ggtgccccct gtagttctcg cgtaaatatc acctcacttt
     1021 ctacttttac caaaggaaat ttagtcgtcg atgcccatat ggggggcttt tttgcagcgc
     1081 aaatgaaatt cgctggatac gacgtcatta ttatcgaagg gaaagcgaaa tcaccggtat
     1141 ggctgaagat taaagatgac aaagttagcc ttgaaaaagc cgatttctta tggggaaaag
     1201 ggacgcgcgc aacgacggaa gaaatttgtc gcttgaccag tccggaaacc tgtgtggcgg
     1261 ctattggtca ggctggggaa aaccttgttc ctctctctgg catgttgaat agccgtaacc
     1321 acagcggtgg tgcgggaact ggcgcaataa tgggttcgaa aaacctgaaa gcgattgcgg
     1381 ttgaagggac gaaaggggtc aacattgccg atcgtcagga gatgaagcgt ctcaacgatt
     1441 acatgatgac tgaacttatc ggtgcgaata acaaccatgt cgtgccaagt acgccgcaat
     1501 cgtgggcaga gtattcagat cccaagtcac gttggactgc acgtaaaggg ttgttttggg
     1561 gcgcggctga aggtggtccg attgaaacgg gtgagattcc gccaggcaat cagaacacgg
     1621 tcggctttcg tacctataaa tccgtttttg acttaggtcc ggcggcagag aaatacacag
     1681 taaaaatgag tggctgccac tcttgcccga tccgttgtat gacccaaatg aatattcctc
     1741 gggtgaaaga gtttggcgtg cccagcacag gtggtaacac ttgtgtagca aactttgtcc
     1801 ataccaccat cttcccgaac ggtccgaaag attttgaaga taaagatgat ggtcgtgtga
     1861 ttggtaacct ggttggtctg aatctgttcg atgactacgg cctatggtgt aactatggtc
     1921 agcttcatcg cgactttacc tattgttaca gcaaaggggt gttcaagcgt gttctgcctg
     1981 ctgaagagta tgcggaaatt cgctgggatc aactggaagc gggtgacgtt aacttcatta
     2041 aagattttta ctaccgtctg gcgcatcgcg tgggtgagct gagtcacctg gctgatggtt
     2101 catatgccat cgcagaacgc tggaatttgg gtgaagagta ctggggctac gcgaaaaata
     2161 aactctggtc gccgtttggc tatccagttc accacgccaa tgaagcgtca gcgcaggtcg
     2221 gctccattgt taactgtatg ttcaaccgtg actgcatgac gcatacccat atcaacttta
     2281 ttggttccgg cttgccattg aaactgcaac gtgaagtggc gaaagaactt tttggttctg
     2341 aagatgctta cgatgaaacc aaaaactaca cgccaatcaa tgacgcaaaa attaaatatg
     2401 ctaagtggtc gctgttgcgg gtctgtttgc ataacgccgt caccctgtgc aactgggtct
     2461 ggccaatgac cgtttcgccg ctgaaaagcc gtaattatcg gggcgatctg gcgcttgaag
     2521 ccaaattctt caaagcgatc actggcgaag atatgactca ggaaaaatta gatttagctg
     2581 cagagcgtat ctttacgttg catcgcgcct acacggtaaa actgatgcaa actaaagata
     2641 tgcgtaatga acacgatctt atctgttcct gggtattcga caaggatccg cagatcccgg
     2701 tctttactga aggtacagac aaaatggacc gtgacgatat gcatgcttcg ctcacgatgt
     2761 tctacaaaga aatgggctgg gacccacagc ttggttgtcc gacccgcaaa acattgcagc
     2821 gtctggggct ggaagatatt gccgccgatc tggcagcaca caatctactg cctgcgtaag
     2881 gaatgggtga gatgaaccat caggatgaat tgccgttagc gaaagtcagc gaagttgatg
     2941 aggcgaagcg gcaatggttg caggggatgc gtcaccccgt tgatacggta accgaaccag
     3001 aacctgctga aatcctggca gagttcattc gtcagcattc tgcggctggg cagctggtgg
     3061 cgcgcgccgt gtttctctcg ccgccctatt cggttgcaga agaggaactg tcagttttgc
     3121 tggaaaacat taagcagaac ggagaccacg cggatatcgc gtgtatgacg ggaagccagg
     3181 atgattacta ttactcaacg caggccatga gtgaaaacta cgcggcgatg tctttgcaag
     3241 ttgtggagca ggacatttgt cgcgccattg cacacgccgt tcgttttgag tgccagacgt
     3301 atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc atattacttc caggaagcgc
     3361 agatcgaagc cgcgatcgcc gcgatggata ttgcacctga atatgctgat atacggcagg
     3421 tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg atttatgacc tatggcaagg
     3481 cgtatggctt atgcgaatgg ttcgaggtag agcagtttca gaacccctga aatttcaggg
     3541 agatgtcatg gatagggtgg acagtagcag ccactgcatt aggggataat cagatgtcct
     3601 tcactcgacg caaatttgtt ctggggatgg gaacagtaat cttttttact ggttctgcgt
     3661 cttctctgtt agcgaacacg atgcaagaaa agaaagttcg gtatgccatg attcatgacg
     3721 agtcacggtg caatggctgt aatatttgcg cccgcgcatg ccgtaaaact aaccacgtcc
     3781 ccgctcaggg aagccgttta tcgatagcac acattccagt caccgataac gacaacgaaa
     3841 cgcagtatca cttttttcgc cagtcatgcc agcactgtga agatgctcct tgcatcgacg
     3901 tttgcccgac aggggcgtcg tggcgtgatg agcaggggat cgtgcgggta gaaaaatcgc
     3961 agtgcatcgg ctgcagttat tgcattggcg cttgtcctta ccaggttcgc taccttaacc
     4021 ctgtcactaa agtggcagat aaatgtgatt tctgcgctga gtcccgactg gcaaaaggct
     4081 ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt gatctttggt cgcgaagata
     4141 gcccagaaat tcaggcgtgg ttgcaggaaa ataaatacta tcaatatcag ctacctggtg
     4201 ccgggaaacc gcacctgtat cgccggttcg gtcaacattt gattaaaaag gaaaatgtat
     4261 gaacccgtcg caacatgctg aacagtttca gagccagttg gcgaactatg tgccactgtt
     4321 cactccccaa ttttggccgg tgtggttgat cattgccgga ctattgctgg tagggatgtg
     4381 gttggtgctg gggctgcatg ccttgcttcg tgctcgtggc gtgaagaaat cagccaacga
     4441 ccatggtgag aaggtttatc tttacagcaa agcggtcaga ttatggcact ggtcgaatgc
     4501 gttactcttt gtattgttgc tggcaagtgg gctgataaat cactttgcgc tggtgggcgc
     4561 aactacggtt aaaagtctgg ttgcggtgca tgaagtttgc ggatttttgt tactggcatg
     4621 ctggctcggc tttgttctga tcaatgccgt tgggggtaat ggtcaccact atcgcattcg
     4681 tcgtcagggg tggctggagc gagcggcaaa acaaacgcga ttctatttgt ttggcattat
     4741 gcagggggaa gaacatcctt tcccggcaac aacccagtct aaatttaatc ccttacagca
     4801 ggtcgcctat gttggtgtca tgtatggatt gctgccattg ttactattga cggggctgct
     4861 gtgtctctat ccgcaagccg tgggagatat gtttcctggc gtaagatact ggttattgca
     4921 ggcgcatttt gctctggcat ttataagcct cttttttatc ttcggtcatc tttatctttg
     4981 caccacgggg cgtacgccac acgaaacctt taaaagcatg gtcgatggct atcaccggca
     5041 ctaacgaatg atcattacac gagcagacct gcgagaatgg cgtatcggag cggtgatgta
     5101 tcgctggttt ctgcgccatt tcccacgcgg cggtagttat gccgatattc atcatgcact
     5161 gattgaggaa ggatatacgg actgggcgga gagtctggtt gagtatgcgt ggaaaaaatg
     5221 gctggcggat gaaaacttcg cgcatcagga agtttcttct atgcaaaaac tggccacaga
     5281 tcccggagag agagcatttt gcagtcagtt tgcccgcagc gacgatcatg ctcgcatcgg
     5341 ctgttgtgag gataatgcca gaatcgcaac cgctgggtat gccgcgcaaa ttgccagtat
     5401 gggttatagc gtgcggattg gaagtgtcgg ctttaacagt cacattggca gctccggtga
     5461 acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata agcagcgctg gagatagcag
     5521 tcggattgcg aataccggaa tgcgagtacg cgtttgtaca ttgggtgaac gatgtcatgt
     5581 cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc gcaaacgcca gaattgccaa
     5641 cagcggcgac aatgtacaca tcattgccag cggtgaagat tcaacaatag tgagcacggg
     5701 cgtggtcgac tcaattattc tcgggccagg gggaagcgcc gcactggcat atcatgacgg
     5761 cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac aatattcgag ccggagtccg
     5821 ctatcgcctt aatgaacagc atcagtttgt tgagtgctga tagctttaca tttgattaaa
     5881 cgaaagacgc cgacgagcac gggatgcagg ctttacgacc gcctgagcta tcgcctcacc
     5941 aatttctgca catgccgtaa gtccctgtac gaatggctgg tcgtgcatca accagggcaa
     6001 cgcgccgaac gctacgcgcc cacgaatttc ttcgggttgc tgtagcgtat aatcttcgcc
     6061 aacatcaggg atttcatccc ctgttttctg taattgttcg cgtagtccag ggaaaggaat
     6121 atctttcact ttaagcggac gctgcccacg ggcatcaata aaaacgtcaa acgagtagct
     6181 attgtcttcc gttttcagga cggtgcgcga ctcattaatt tccatttcgt agtcttcacc
     6241 gagggcgaga atatgaatga ttcccgcttc acgtaaggcc agtagtcgac gaatagactc
     6301 tgaagggatt gccgcataat tatcgatgaa aacccgggca aggcctttac tgaaccgttt
     6361 atggtcgtgt tcattcagat gaggaacaat ttcctgtacg gcttcatgca ggcgcagaat
     6421 gacataacgc cagggaacag tatgtttttc tcgtttattg cgttcgactt cctggagatt
     6481 tttttctgcc cagtcgaatg ggtcgcgttg tttgcgctcg gcaaaccagg cttgagcaaa
     6541 ggaatcgaca ttcaggcttt ctaaggctat gcgttggctc cagtctggat cagcaaattt
     6601 gatttcctct actatcaatc taaatacccg atccaaaagg cccacttcgc ctttttgaat
     6661 ctcagcattt aatgcttgat cggtgacgat gtgtaagggc tcgtagggaa tagggcaata
     6721 gaaatcggct tcgggtaaaa tacccgtacg cgacatcaac gtaatattta gcttttcact
     6781 tgcgttatcg cggtgaaaaa cgacgtgttg tttatcatct tcaatgaacg aaccatgctg
     6841 aatagccact gccattgccg catccagtcc gctcaaggat gttcccataa tacccacgtt
     6901 acacgcatcg acctttgctt ccatcaggcc tgaccacggg ctgggaaaat acgttcgggt
     6961 tgcttcttct tcatcaggcc agacgtgacc cgtggcgatc accgctaaat caaacgtctc
     7021 gctgggtaaa tcctgatttg tagcgagcat gacgccagca tttgtaattt gcagatcggt
     7081 aacctggcat gattcataaa cagccactgc aaatttttgc tgtcgtgcct ggtctactaa
     7141 tcgtaaaaat tgatcgcgga aatattcgcc cagcagaatt cgcggtaaaa actgacgatc
     7201 gtgcaaggtt tcttttttaa cgccataacg ctggagatgg ctggcttctt gcttttgtag
     7261 ccattcgaga tacgtacaat aaatcggcgg tatttcaata ctggcaatat ttgccagcat
     7321 catttttgag ttttcctcat cactgtatgg catcccgaca ccggcttcat cagcctgctc
     7381 gaagatagaa atagaaagtg gagtttgttg ctgtagaagc gagaataagg tgtagatccc
     7441 cgtaggcccg gcacccacaa tagcaatttt tttcattgtt acttacctgt acgcattctt
     7501 tcgaatttag tgttatcaga taagtttggt tgagattttt agaatttgag aaatttcaga
     7561 cagggatgaa aagggcgcta accgcccttg attaaattct gtcaggcgag ttttgcctga
     7621 ttgatttgac taagtggctc attgacatcg aaaactttgg cgacaacttc ctcaacgccg
     7681 agatttttca gtcgggcagt atgtttttcc agataggcaa gggcgctttt ttcattagta
     7741 aaaaggtaga tcccaccggc ttcgtggttc ttttcacttt ctgtccatac cttccacaga
     7801 aaaccaggtt cctgattaat cgactcagca agcggtttaa gctgctcagc cattgcgtcg
     7861 ccaaatgggc cattgaaaga aaaatgaagt tgtaacaaag ttgccatagc gattctcctt
     7921 ctgataacgc atacgaagac agtgtaaatc ttttaacgta ttggatatag agaagaaaca
     7981 gagggaaaaa ggagaagacc agattaagaa aatctggtgc gtccgagtgg actcgaacca
     8041 ccgaccccca ccatgtcaag gtggtgctct aaccaactga gctacggacg caggatggtg
     8101 cgttcaattg gactcgaacc aacgaccccc accatgtcaa ggtggtgctc taaccaactg
     8161 agctatgaac gcaacgttgt aggtgacaac ggggacgaat attagcggca gagtgggaag
     8221 gtggcaagag gcaaaacgta attttctgcg ctatttcgac cgtttgcaga gcttttaagc
     8281 aaattggcta tattttgttg atttgcaagg gcgattttta ttcaggatcg catttacatc
     8341 agatacaacc caataatccg tgtatcagag tttaaacaga gttgtttgcc accatctcaa
     8401 aatctgtcgt ggataaatcc tccgtagcct ttacacttag gtagcaataa aggcacacag
     8461 atgcagcaaa ggaataaatc atgggatctg atgcgaaaaa cttgatgagc gacgggaacg
     8521 tgcaaattgt taagaccggc gaggtcattg gcgcgacgca acttactgaa ggtgagttaa
     8581 ttgttgaagc tggcggaaga gccgaaaata ccgtggtcac gggggctggc tggttgaaag
     8641 tggcaaccgg tgggatcgcc aaatgcacac agtacggcaa caatggcacg ctatcggtca
     8701 gcgacggtgc cattgccaca gatattgttc agtccgaggg aggcgcaatt agtctctcta
     8761 cgctcgctac ggttaatggc cgccatcccg aaggtgaatt cagcgttgat aaaggttatg
     8821 cctgcggttt gttgctggaa aatggcggta acctgcgtgt actggaaggc catcgcgcgg
     8881 aaaaaattat tctcgatcaa gagggtggcc tgttggtcaa tgggacaacc tcagcggtcg
     8941 tggtagatga aggtggtgaa ttgttggtgt atccaggtgg ggaagccagc aattgtgaga
     9001 ttaatcaggg cggcgttttt atgctggcgg ggaaagccag tgatacattg cttgctggtg
     9061 gcaccatgaa taatctcggt ggtgaagact ctgacactat tgttgagaat ggatccactt
     9121 atcgtctggg gacggatggt cttcagctct acagttccgg taagacgcaa aacctgtccg
     9181 ttaatgtggg tggtcgggct gaagtgcatg ccggtacgct ggaaaatgcg gtaatacaag
     9241 gtgggacagt catcctgttg tcacccacca gcgcggacga aaattttgtc gtagaggaag
     9301 atcgcgcacc ggttgaactg accggtagtg ttgcattact ggacggcgct tcaatgatta
     9361 ttggctatgg cgcagatctg caacaatcaa cgattactgt acagcagggc ggtgtattga
     9421 ttctcgacgg cagtacggta aaaggtgaca gtgtcacttt cagtgttggt agcatcaatc
     9481 taaatggcgg aaaactgtgg ctaatcactg gtgcggcaac gcatgtgcaa ctgaaagtga
     9541 aacgcctgcg cggagaggga gcgatttgcc tgcaaaccag tgcgaaagaa atttcacctg
     9601 acttcatcaa tgtgaaaggg gaagttactg gtgatataca cgttgagata acagatgcca
     9661 gtcggcaaac tctgtgtaac gcactgaaac tacagccaga cgaagacggg attggcgcaa
     9721 cgctccagcc tgcgtaaaaa cacaggcgca acccgtgaag gttgcgcctt gtctttacta
     9781 gcgagctgct cgttgcagaa tgatggctga cggcagacgt tgcaggaacc gcatacgcag
     9841 catcatcata atggctgccg acgtcaggcc aataataaag cctatccaga agcctgctgg
     9901 ccccataggt tcaacgacca gatcggtcag tgccagaata tagccgcttg gcaagcccag
     9961 cacccagtaa gccgtaaagg taatatagaa aatggaacgc gtatctttat atccacgcaa
    10021 aatcccactg ccaatcacct ggattgagtc agaaatctga tataccgccg ccagcaacat
    10081 caaatgcgca gccagcgtta caacctcggg attgtcgttg tacaacaggg cgatttgctc
    10141 ccgcagtgaa accgtgaaaa tggccgtcag ggttgccata cagacaccca ccataagccc
    10201 ggtccgcgca gcggtttgcg catccagcgt tgagccctga cccagacgat aacctacgcg
    10261 gatggttacc gctgccgcca gcgacatggg aagtacgaac atcagtgaac taaagttcag
    10321 ggcaatctga tgtcctgcga catcaacaat accgagcgga gacactaaca gagccacgac
    10381 ggcaaacagc gttacttcaa agaacagcgc cagcgcaatt ggcaaaccga gttgaatcag
    10441 tcgtttcata accgcaggat cgggcttcgc agtgcctttt tcgttacgaa tatcgcgcat
    10501 cgagcgggcg cgtttaatgt aagaaaccat ggcaaggaac atgacccaat acaccgcagc
    10561 agtagccacg ccacaaccaa cgccaccgag ctcaggcata ccgaaatgac cgtaaataaa
    10621 gatatagttc accggaatgt tcaccagcag gccgataaag cccatcacca tacccggctt
    10681 ggtttttgcc agaccttcac actggttacg ggcaacctgg aagaaaagat agcccggcgc
    10741 gccccatagc aacgcacgca gataacccac ggctttgtcc gccagagccg gatcgatgtt
    10801 ttccatggag cggataatgt aacctgcatt ccacagcacc agcataatga gaacggaaac
    10861 aaaacccgcc agccagaaac cttgtcgcac ctgatgcgca atgcgctcac gtcgaccgga
    10921 accatttaat tgcgcgataa ccggcgttaa tgccagcagc agtccgtgac caaagaggat
    10981 cgccggaagc cagatagaag taccgatagc gaccgccgcc atgtcggtgg cactatagcc
    11041 gcccgccatc acggtatcga caaaacccat cgcagtttgg gcgatttgcg cgagaatcac
    11101 cgggattgct aatgctaata acagacgcgc ttcactgata tacttctgca cgtgaacacc
    11161 ttttatttgt agttatatga gagactaaaa agccgcctga atgggcagca aaaagaagaa
    11221 gcaggggaaa ttcagtctat tgtagcgagg atttattaat tctccagtga aaaaatagtc
    11281 attcttaccc cttcactggt aagggcgttt gccacctgct attgtgctga acagaatgtc
    11341 ttaactgatt tcaggagttg taagtatgtt tacggggatt gtacagggca ccgcaaaact
    11401 ggtgtcgatt gacgagaaac caaattttcg cacacatgtg gtggagttac ccgaccacat
    11461 gctggacggt ctggaaaccg gtgcttccgt ggcgcataac ggttgctgcc tgaccgtgac
    11521 ggaaattaac ggcaaccatg tcagttttga cctgatgaaa gaaacgttac gcattaccaa
    11581 tcttggcgat ttaaaagtgg gggattgggt aaacgttgag cgtgcggcga aattcagtga
    11641 tgaaattggc ggacacttaa tgtcaggtca tattatgacc accgctgaag tggcgaaaat
    11701 attaacctcg gaaaataatc gccagatctg gtttaaagtc caggatagtc agttgatgaa
    11761 atatattctg tacaaaggat ttattggcat cgacggtatt agcctgaccg tcggtgaagt
    11821 cacgccaacg cgtttttgcg tccatttaat tccggaaaca ctggaacgca cgactcttgg
    11881 gaagaaaaaa cttggcgcac gcgtcaacat tgaaatcgat ccacaaactc aggcagtggt
    11941 agatacggta gaacgagtgc tggcggcacg agaaaatgcc atgaatcaac caggtacaga
    12001 agcctgatct gaaatcgccc ccggagaggg ggcgatagaa tagcctttag cgagccactc
    12061 gaaggccgtt ttcaacaccg cgtgagaaca cgacctgcca gagctgaata tcacgggcgc
    12121 ggaaagcacc cgcacaggca ttcagataat aggtaaacat ccgtttaaag cgttcactgt
    12181 agttatccgc aatttctggc catgcggcga ggaatcgttc ataccacgcc atcaacgtag
    12241 tatcgtaatc agcaccgaag ttatgccagt cttccatcac aaagtggggt tcgctggact
    12301 gagcaatctg gcgtacagag ggcaggcaac cgttcggaaa aatatattta ttaatccagg
    12361 gatcaacatt cagatcggtt tttttcgaac cgatagtatg gagcaggaat atgccttccg
    12421 gtttcaaatt acgatccacc accgcaaaat aggtatcgta atttttcggt ccgacgtgct
    12481 cgaacatccc cacagaaaca atacgatcaa actggtcgtt caggtcacga taatcttgca
    12541 gcaaaatggt gacatccagg ccttcacagc gttcctgagc cattttttgc tgttcggcag
    12601 aaatggtgac gccgaccacg cttacgtcat aattagacgc catgtagtgc gccagtccac
    12661 cccagccaca gccaatatcc agtacgcgca tccctggttt taactgcaat ttttcacaaa
    12721 tcattttgag cttcgcctgc tgggctgatt ccagattatc ggcatctttc cagtaagcac
    12781 aggaatattg catgaaggga tcaagcatgc ggctgaacaa gtcattaccc aaatcgtaat
    12841 gctctttgcc gactatccag gcacgttttt tactctgcag attgaagaga cgagcgccgg
    12901 caatacgcag cgtgtctttg aaatgatggg ggagttggtt ctcgagacct gcgcgtaaga
    12961 ctttgctaaa aaacatatcc agtcggtcac attcccacca gccatccata taactttcgc
    13021 ctaaccccaa cgatccttcc tgcagaacgc gtttaaaaaa atcggggttt ttcacacgaa
    13081 tatccgccgg ggcagaaccg ttaatggcta taccggcacg gctaagtaat tcgttggcga
    13141 tacggtacca gttgtcatcc ggtacactga cttcttctat acacgatgaa ctcatagttt
    13201 ctccatcact ggtcggtgat cagaacctta aaacagcgta gacgcttttt gggctttgtg
    13261 agaaatctcg cagaaaaaac cgcgagtcta taatccgacg tagaacagag ggaaaggagg
    13321 aacccttgcc gaaatgacca tccatagtga gtcgggaacg atcctgtccc cgtaaaaaca
    13381 taatatttat cgtagcttta acaactcgaa attagtatag gccgctaatt tcaatcatac
    13441 aacaaaattt tgttagcagc ctaagtataa tcatatcgat atagatcagt gtgattcgct
    13501 atgagcgact tcggcattgc catgattctg acagtctact tcttcacaac gttgcatcat
    13561 gtaacccaga gcgaccagca ctactgttga taacatcacg ctggtggtgg tgagcaatgg
    13621 cgtgctgata ctgatcagcc aggaaactac cagacttgcg aggaagcaca gacccagttg
    13681 tagagtgttc tgcaacgctg cggcgcgacc ggttgcatgt gggaagggac gcagcgcctg
    13741 ggcgacaaca atcgggtaga tcgcgccatt ggcaatcgcc atcacacaga atgggatcag
    13801 gatttcgact agcgacacat ggctaatgaa gcccgcagcc caggtcgcaa tgacgctgac
    13861 agcaaacagt accagcaacc acggtaataa ctgcttgcct tgccatttct gcagtgcggc
    13921 gcgacagcca taaccaccaa tcagaaacgc gatagtttgc gggacataac ttaaaccaat
    13981 aactgccggg ctgtagccca tttcactaag gatgaacggt gagccggtca gccatgcaaa
    14041 aaaactggct gaacaggccg catatatcag cacgttgccg cgataggttt tagaacgtag
    14101 caggtcggta aaggtcagac catcctgact attgttacgg gccttcgtcg tgggtttgag
    14161 ccagaaaata ggcagaatca gcaccacggt aatggcaaac agggtggcga aaatcgcctg
    14221 ccaggaaaaa tggaccagca gccagcttcc taacagagga gccagtgccg gagatagacc
    14281 caccagcggc atgatggtcg caaaaatacg gttaactttc tgtgaaggat aataatccgt
    14341 cactaacgct tgccagataa ctgccgcggc gcagacaccc acagcctgta caaaacgcaa
    14401 taccagcagc gtagcggcgt tttctaccca cagcatcccc agactaccta acgcaaaaat
    14461 tgtcaggccg attaataata ccggtttacg accataacgg tcggagagcg gcccccacag
    14521 aagctgggct gcggcaaaac cggcaaggaa cagactaagg ctggcactga cagcagacgc
    14581 aggcgtttgc aggtcggcct gtatggcggc gaaagcaggc agatacatat cggttgccag
    14641 aaaaccgagt acgctcaaac ccgccagcca gactaaaaat cttttcccag gttgcatgta
    14701 tattctctaa atctctcaat taaacaccgc cgcaaagtgt agggagtgaa attagtgttg
    14761 tgaaacggta atatttgatg actggtttca aaaattttgc aggcagacta tgtggtcaga
    14821 atattcactc gaagttgttg atgcggtagc gcgtaatggt agttttagcg ctgcggcaca
    14881 ggagttgcat cgcgttcctt ctgcggtcag ctataccgtg cgtcagctgg aagagtggct
    14941 ggcggtgccg ctgtttgaac gacgtcaccg tgatgtggaa ctgaccgctg ccggggcgtg
    15001 gtttctcaaa gaagggcgct ctgttgtcaa aaaaatgcag atcacccggc agcaatgtca
    15061 gcagatagcg aacggctggc gcggtcagtt agctatcgca gtggataata ttgtcaggcc
    15121 agaacgtaca cggcagatga tcgttgattt ttatcgccat tttgatgatg tcgaacttct
    15181 tgtctttcag gaagtgttca acggtgtctg ggatgcgctt tccgacgggc gcgtggaact
    15241 ggcgattggc gcaacacgcg cgattccggt aggcggtcgt tatgccttcc gcgatatggg
    15301 gatgctaagc tggagttgcg ttgttgccag ccaccacccg ctggcgttga tggatggccc
    15361 gttcagcgat gatacgttgc gcaactggcc gtcgttggtg cgcgaagaca cctcgcgaac
    15421 gctacctaaa cgtattacct ggttgctgga taaccaaaaa agacttgtcg tgcctgactg
    15481 ggaatcatcg gcaacctgta tctcggcagg attatgcata gggatggtgc caacacattt
    15541 cgccaaaccg tggcttaatg aggggaagtg ggtagcacta gaactggaaa accccttccc
    15601 ggattcggca tgttgtctga catggcagca aaatgatatg tcgcctgcgt taacctggtt
    15661 gcttgagtat ctcggcgata gtgagacgct gaataaggag tggctgcggg agccggaaga
    15721 gactcccgca acgggtgatt aacgacgata gtcgcggaac gggccgtcag ccacggagcg
    15781 gcgttcaatc aagcgcggat gcacttcaat ggactgcggt tcttcgcgtt tattgacgat
    15841 acgatccaac agcatgttga acgccgtttc gcccagcgaa tcttttggct gatggatcgt
    15901 ggtcagcgct ggcgtaaaat agcgggcgtt gcgcacgtta tcataaccga tcagcgaaac
    15961 atcctgcggc acgcgcagac ccatttcatc agcagcacaa agtgcgccca ttgccatgat
    16021 atcgccacca cagaagacgg cagtagggcg atgcggttgc gacagtattt gctgcatggc
    16081 gcgataaccg gattcaggtt caaagtcacc ctgtacaatc cagctttccg gcactttgat
    16141 catcgcttct tccatcgcct tcataaaacc ggcaaggcgg cctgcgccgg tgttacgttc
    16201 cagcgggcca gggataacgc cgatttcgcg atgaccgcgt tcaatcagat aacgcccggc
    16261 catgtagccg ccttcgaatg cgttatcaat gaccgcatcg gtgaagtcag cttttgcttc
    16321 accccagtcc atcaccacca ttgggatatg gcgatactct tccagcatcg ccagcaacgg
    16381 ctctgggtac tcagaacaca tcaccagcag accatcgacg cgtttttgcg ccatcatcga
    16441 cagataagcc cgctgtttct caagattgtt ccacgcattg cccagaatca gggtgtaacc
    16501 tttctggaag caattttttt caaccgcttc aatgatctcg gcaaaatagg ccgcttcgct
    16561 gctggtcgcc agcaaaccga tagacttggt gtggttaacc ttcaggctac gcgccaccgc
    16621 gctaggggag tagtgtaatt ctttaatcgc tgcccacacg gcgttgcgcg tttcttcagc
    16681 gacgaaacgt gttttgttga tcacgtgtga cacagttgta gtggaaacgt ttgctcgttt
    16741 cgctacatct tttattgttg ccattccatt tcactccaga ccctaacttc agctcctgca
    16801 aaatcgcaag gtaaacgttt gcctttacac accttacgca caacaaaagt gttgcggtac
    16861 gccggaaaaa cgacacggag cgtcaggaag gggtcaatgg ccagtacgca aatagtgtaa
    16921 gcgtggaatt ttggccgatc ttgacgaaaa ggggaagtgg taaaaacact tatcattata
    16981 aatgacggga gattttttca tcacagtgtg taaaaatgcg taatatcact ttttgtgtgg
    17041 taataaaaaa ggagaaaact tgatgagcac cgaccttaaa ttttcactgg taacaacgat
    17101 tatcgtcctc ggtttgatcg tggtcgtggg tttgactgcc gcgctgcact gatttcctct
    17161 cgagggagcg cgttctccct cgcatctcct tcctgtttgt tagcaaaatg gcaaaagtgt
    17221 tttctctttt tgttattttc atttttttgt gattgctgtc atgctttcgc cactttaatg
    17281 aatatcgcgt aatgacgcga agaggtggag ataaggcatg aaaattaact atccgttgct
    17341 ggcgctggcg attggcgcgt ttggtatcgg gacaacggag ttctcgccaa tgggcttgtt
    17401 gcccgtcatt gcgcgcggtg tggatgtctc gattcccgct gccggaatgt taatcagtgc
    17461 ttatgcagtt ggcgtaatgg ttggcgcgcc gctgatgacg cttctacttt ctcatcgtgc
    17521 ccgccgcagt gcgttgattt tcctgatggc gattttcacg cttggcaacg tactttccgc
    17581 catcgcgccg gattatatga ccctgatgct ttcacgcatt ttgaccagcc tgaatcacgg
    17641 ggcatttttt ggtttgggtt cagtcgtggc cgcaagcgtg gtgccaaaac ataaacaggc
    17701 cagcgcagtt gccactatgt ttatggggtt aaccctggca aatatcggtg gcgtgccggc
    17761 ggcgacctgg ttgggtgaaa ccatcggctg gcggatgtca tttctggcaa cggcggggct
    17821 gggagtgatt tcaatggtaa gtctgttctt ctcattacct aaaggtggtg caggggcacg
    17881 acctgaagtg aaaaaagagc tggcggtatt aatgcgtccg caggtgctgt ctgcattgct
    17941 gacgacggta ctgggagctg gtgcaatgtt taccctctac acctatatct ctccggtact
    18001 gcaaagtatt acccacgcaa caccggtgtt cgtcacagca atgctggtgc tgattggtgt
    18061 cggattctct atcggtaact atctcggcgg caaactggca gatcgttcag ttaacggcac
    18121 gttgaaaggc tttttgttgc tgctgatggt gattatgctg gcaatcccgt tcctggcccg
    18181 caatgagttc ggcgcagcta ttagcatggt ggtgtggggc gctgcaacct ttgcggtcgt
    18241 accgccgtta cagatgcgcg tgatgcgtgt cgccagtgaa gcgccaggtc tgtcttcatc
    18301 agtcaatatt ggtgccttta atcttggaaa tgcgctggga gcagctgctg gtggtgcggt
    18361 aatttccgct gggctgggat acagctttgt gccggtgatg ggggcgattg tcgcgggact
    18421 ggcattattg ctggtgttta tgtcagccag aaaacaacct gaaacagttt gcgttgccaa
    18481 cagctaatta aacaaaaacg cagaaggata accttctgcg ttacagttgc tggcaacgtg
    18541 cgatttatct tgccgaatgc gctgttaacg cctcatccgt gttggcatgg cgcgcgaagc
    18601 gcatcaggta atgttgcatt tgccatcagt tattatgcag cgagattttt cgctacgaat
    18661 tcccagttca ccagcgccca gaagtgctcc agatagccag gacgtgcatt gcgatagtcg
    18721 atgtaataag cgtgttccca gacatcaacg gtcagcagcg gagttgcatc ggtggtcagt
    18781 ggagtacccg cgttagaggt tgaaacgata gccagtttgc catcgctgtt tttcaccagc
    18841 caggtccagc cagaaccaaa gtttttgatc gctgcatcag taaactgagc tttgaaatcg
    18901 gcaaagctgc caaaagatgc ggcgatagct tcagcgactt ttccagtcgg ttcgccaccc
    18961 gcgttcggtg ccaggcagtt ccagtagaaa gtatggttcc agacctgagc tgcgttgttg
    19021 aatacgccac cttcagagct gcgaataatc tcttccagtg atttaccttc aaacgcggta
    19081 cctttaatca ggttgttcag gttagtgaca taagtctgat gatgcttgcc atagtgatac
    19141 tcgatggttt ccgcagaaat gtgcggtgcc agagcatctt tagcatatgg tagtgcaggt
    19201 aattcgaatg acattgctac tctcctttat tattaatttg catacgtaca atagccttat
    19261 tgtgcgtatg gatagggtag caaattaaaa ggtgagacaa aagatgaact taccctgttg
    19321 ccgtaacaac agggtaaagt tttagcgaag tgtttttggg gtcattaccc gacgagcgcc
    19381 aacatagtgg cgctgccagt agtcttcact gagagaagtg atttgaattt cttggcccgt
    19441 gcgcggtgac tgaataaatt tgccgttgcc gacatacacg ccgacatgat cggctgtacc
    19501 gcgtccctga gtacggaaga agaccaggtc gccgtttttc agttcactac gttcgatagg
    19561 tgctgcatca cgcaggtgat acatttcatt cgccgtacgc ggaatacgaa ttttcaccaa
    19621 atctttataa gcgtaataaa ccaggccgct gcaatcaaaa ccggtacgcg gtgagctgcc
    19681 gccccaacga tatggcttac caatttgttg catcagttta ttcattgcca ctttcgtggc
    19741 tttttgcacc ttcgctttgt gcgcgtcggc aagggttcct gacgcggcat ttttgacttt
    19801 cacacaatgc gatttatagc ccttacgttt ggtacacttt tcggtaaagt tggctgaggc
    19861 cgttttattg acggcatgtt tgctgcgagt tttggcgttt ttagaagcgg taatggaaga
    19921 ttttttaacg gaagaagcgg ttttgctttt ggtcgtactt ttactggcgg cttttttcgc
    19981 tgttttctgg ctggctttct gggtcttttt ggtggttgcc gtgcttttct ttttatctgc
    20041 ttttttggtg atatgagtag tagcagaact ctccctggct tgctttgaag catgggccat
    20101 aggcgttaaa ggcagggtgg taaaaagtaa agcacagagc gtgatcgaaa tacggtttat
    20161 ccgcgccact gagcgtccct ctgttaaggc aggtctatca tgcctgcgtc tgatttacaa
    20221 aacttgatga ttctaatgca tagaagaaga gaaagttaat agcttttatt gttaaaaaat
    20281 acgtttcgtt ggcgagcgca aaaaaatgca gcagtgaaag ctaaaaatgg ttataaatac
    20341 gccagtttgc cgtgcgtcag ggaagaaacg aatatttgaa atgcaacaaa tagagcaaag
    20401 ggataaaata gcaaaagcgt gatgaaaatg ttattttccg atgccgcact gacccgctac
    20461 aatgtcagat tactgccgta aagaagtttg aggaagcaag acaatgagca ccactatcga
    20521 aaaaatccaa cgccagattg ctgaaaaccc gatcctgctg tacatgaaag gttcaccgaa
    20581 actgccgagc tgcggtttct ctgcccaggc agtccaggcg cttgccgcat gtggcgaacg
    20641 ttttgcctat gttgatatcc tgcagaatcc ggacattcgt gcggaactgc cgaaatatgc
    20701 taactggccg accttcccgc aactgtgggt tgacggcgag ctggtcggcg gttgtgatat
    20761 cgtgatcgaa atgtatcagc gtggcgaact gcagcagctg atcaaagaaa ctgccgctaa
    20821 atacaagtct gaagagccgg acgctgaata atccgtcctg caatactgac ggatgcgaac
    20881 gggcattcgt cagtcctttc tctatcccca atccagccct tgtggcgaac ttgaaaatcc
    20941 cgcctcacgc agcagcgtaa acatcggcgt ttgccggacc ggtaggtcat tcacttctgt
    21001 tagcgtaaag cgcagccgtg gttcgcgacg cagtgcggta gtcagcgcgt ggaaaatctc
    21061 tggggcgagt aattcctctt tttcctgcca gaccagcatt tttttgccac cttgcgccag
    21121 atagagtaac aatttgccgc cagaaacgac caccagcgca cccgcccgac gcgttggtat
    21181 cagcgttgcc ggatgtgcag gccagggcag aagatttccc catgcattag ccggatcgtt
    21241 ggcagagagc gccactggtg tatagtggcg cgtttgcgtg gcttgtgtcg ccagatcgcg
    21301 caatcggtca atagtcagac gttcagcgaa ttgcgcgcca cccagacctt ctacaaaacg
    21361 acctcgcata attcgcccgg agtcttccat acttcgacaa agcgtttgca tcgatggaaa
    21421 cccgccaggg atattttcgg ctatcaccgc ctgacgactg atgatgccgt agcggtcgag
    21481 catattttcc gccagcgcca gcatcctttc ggtatcgttt agtggctcca cctgcaataa
    21541 cgaccagcgt ccagcaagct ttggtgtgtt gtaagatacc agcggcgaga cgggttgcgc
    21601 atagacagga cgtccacgac gagcccggtg actgcggcga gttgaggtgc gtgtgttggt
    21661 actgctgcgg gtgagggcgc gtaacggtgc ccaaatgtcg ctggtgatga cgccttgcca
    21721 gaccagcgcc cataacgcct cttgcatggc tgagggatca accgattcgc cgattttatc
    21781 gcgtatccgt tggctgattt gttgtgcaaa ccaggctcct ccgtcagcca gaaccgtgac
    21841 tatcgcttgc tgcagcgccg aactattcgc ctgattcgct tcggcgggag tgaacgattc
    21901 tgcggcatat tcctgtagat gcagtgccac cagaccgtca tcttcaccca gctttttttg
    21961 ccccgaccag ataaccgcac cggttgccag taattcatcg agcatttctg gcgaatagtc
    22021 gcgtacgcgg gcaggcagga tctggctttc ccagagtgag gcgggtaaac cgactccggc
    22081 aagctgttcg atcacccgca tgacgccatc cacgccctca taaacgcctg gcgatgttga
    22141 ggcaaagagt gccgggctac catcggtggc gggtaatacg ccctgacgtt ccagcagcaa
    22201 tcgcgcatag gtcgtggccg caacgggacg cgtcgcttct ctggcggctt gcagcgagcg
    22261 caaacgcaga cgacgaaata cttcatcgct cacccagatg tcttgttgca gattcatcac
    22321 cagcccctgt tcgcgcagtt gctgaagttg ctcttcgaca atggcaatac cgagactaaa
    22381 ctcatgagcc agttgttcag acgtgaccag agcatgagcc cggagatagc gcagaaagag
    22441 gtcgcgaagc gggtaactga ctcggtgcaa ataaatctct ggcagtgact ctggtagtcg
    22501 tacgccgaga gcatcgcgca gcctggcggc atcatccata caggcaagac gctcctgccc
    22561 gctaatcatg gtcgggaaga ttcgttttac tgcaagaaga ttttccagat acgacgcaat
    22621 ctcttcactg ctgcctgtat gtcgttgcgc caggtcttca acggtcattg gccccagttc
    22681 gcgcagcagg tcgaacaatc cttcttcacc tttcgctctt ctgccaggag ccagtcgttg
    22741 caactcttct tccacctggc gaatgacctg tgggtcgagt aattcccccg gatcgacctg
    22801 tccgagtaga ttgcgcagta actcgctgtc cagcgacagt acggatgccc ggcgctctgc
    22861 cagcggggcg tcgctctggt acataaattc cgcgacatag ccgaacaata aactggcggc
    22921 gaaaggcgaa ggcgtagtgg tcgttacatc ggatatttga atttcgccac cgttcaggcg
    22981 acgcatcaaa cgttccagcg cgggaagatc ataaacatct tgcagacatt cgcgtagggt
    23041 ttcgagaatg accggaaaat ctggatatcc ctgagcgatt tccagcaact gactggcgcg
    23101 cagccgttgt tgccagagcg gggtgcgatg gcccggcgtg cggcccggca ttaataatgt
    23161 ccgcgcggcg cattcgcgaa aacgggcggc gaaaagtgcc gagctgccta ccgcctcgcg
    23221 gacaatttgc agcaactttt ctggttcaaa caaaaaaatc gcggcatcag gcaatttacc
    23281 atcggtgtca ggaatgcgcg caacaatgcc gtcatcactg gcaaccacag acgcgtcagc
    23341 gccccatagt gcatgtattc gcccggcaat cgccagcgcc cagggttcat gcacccgtct
    23401 tccataggga gagtgcaaaa taatacgcca gtcaccaatt tcatcacggc accgttcgag
    23461 gaccagatga cgactccccg gaacgatgcc cgtcgcgttg cgctgctcgt caatcaaccc
    23521 ctgaatattg gcgatcgtat tttcttctgc caaccacggg ggaatagtcc cggaaaagaa
    23581 cgcgccatcc gccagtaaat ggagaaaatc gccgatcatc tcgcctaatt ctgccggacg
    23641 tccgttacct tcaccacgcc agaagggaag gcgggcagaa cgacctggag caggagtcac
    23701 aatcacctga tcgcgggtga tttgctggat ccgccatgag gtagcgccga gagtgataat
    23761 gtcgttcacc cgcgactcat ataccatctc ctcatccagt tcacccaccc gccgcgaacc
    23821 ggcctgttct tcaccttcgg gtaataacac gctatacatg ccacgatccg gaatggtgcc
    23881 accgctggta accgccaaca gctgagcgcc aggtcgggcg gtcaatatcc cggtctccct
    23941 gttccagacc agttttggtc gaaaagcaga aaaatcgcca gagggatagc gcccggaaag
    24001 catatccagc gtgacgtcaa aaacacgtct tggcagatct ttccacggtg cggcacggcg
    24061 tacgcgggag taccattcgt ctgcctgtaa tgcatccatc gccgcggcgg caacggtttg
    24121 ctgcgcaagg acgtcgagtg gattatgcgg cggtgtcagg ttttccagcc tgcctgcgaa
    24181 catacactct acaatgactg cggaatcgac taagtcacgc ctggttcggg ggaaaaaaag
    24241 ccctttagat acaccgccaa cctgatgtcc ggcgcgacca atgcgttgta acccactggc
    24301 aacagaaagc ggcgttgcca cctgaatcac cagatccacc gcgcccatat caatccccag
    24361 ttcgagactg gaggttgcga ccacacagcg taattcaccc gatttcaacg cctgttcggt
    24421 gattgctcgt tgttctttag agacggagcc gtggtgtgag cgggcaataa aaatgtcgct
    24481 actttgtaca cggttagagg ttgcgccgga ggtcgactcg aaatgggccg catcaacggc
    24541 gatagatggg gaacgctgta agcgtgcggc gtaaagctca tttaatcgtg ccgtcagttt
    24601 ttccgccagc ccgcgtgaat tagtaaagac aatggtcgag cgatggcgca aaacttcatc
    24661 aaggataccc gtttcaatat atggccagat ggagccttcc cggccggcat ggctgtcttc
    24721 gccggtgccg ctggcgaccg atgagacatc atccatattg gcgaccggta cgacaatccg
    24781 tatctgcgga tggcgcattg cgggcgggtt gactaccgta accgggcgat cgccaccaag
    24841 aaatgctgcc acatcgctgg ctgaacgcac agtggcagaa aggccaattc gctgtgctga
    24901 ggtgtggagc agcgtatcga gccgctccat acttaacgcc agatgcgcac cacgtttact
    24961 gcccgctacc gcgtggactt catcaataat taccgtttcg acgccgcgta gcgtttcgcg
    25021 cgcgcgggag gtcagcatca gatagagtga ttcgggtgtg gtgatcagaa tatccggcgg
    25081 attacgggtg agtttgctgc gttcctgtgc aggcgtatcg ccagtacgga tccccacgcg
    25141 aagattgact tccgtttcgc cgcgccgccg ccgttcatcg gcaataccct gtaacgggat
    25201 ctgcaagttg cgctgaacgt cggtgcccag agctttgatc ggtgaaatat agaggatgcg
    25261 tgaggtttta cgcttatgcg cctcgcgggt atcttcgccg ccttcgcgga agagctgatc
    25321 gagggcgtag agaaatgctg ccagcgtttt cccggagcca gtcggtgcaa tcaccagcgc
    25381 atgttcgcct cgcgccgcca catgccaggt ttgcgactgg acagcggtcg gctgtttaaa
    25441 ggcgcgaaga aaccagtcgc gggtcgccgg tgaaaacacg tccggcaggg gcgatgaagg
    25501 gtttagatta tctgccatta acgctattcc ccgtgaatat catgcatgat gcgtggaata
    25561 gcctcactat agatgcctga atcattgcat gtcatcaggc atcgactcga ttacacctct
    25621 tcggcggcag gtagcggcca gcctcccaga cgtttccagc ggttgacgat ttcacaaaac
    25681 agcacagcag tgcgttcggt gtcgtacagc gcggagtgcg cctgggtgct gtcgaagtcc
    25741 atgccagcgg tctggcaagc ctttgacaat acggtttgtc cgagtgccag cccggccagt
    25801 gcagcagtgt caaaagtggc gaaagggtgg aacgggttac gtttcagtga ggcgcgttct
    25861 gcggcggcca tcataaagct gtgatcgaaa ttggcgttgt gcgccaccat aatggcgcgg
    25921 ttacaaccgc tcgctttaat acctttacgc acaactttaa aaatttcgtg cagcgcctcg
    25981 tattcgctga ccgcgccgcg atcgggatcg ttcgggtcaa tgccgttgaa ggcgagggcc
    26041 tctggctgca aatttgcgcc gacaaatggt tcgacgtgga aatgtaatgt ggtgtccggc
    26101 atcagccagc cttgttcatc cattttcagg gtgatggcgg caatctcaag cagcgcatcg
    26161 gttttggcgt taaatccagc tgtttcaaca tcgatcacaa caggataaaa accacgaaaa
    26221 cggtcgcaca gaccggtaag ttgagcgtta tcggacatct gattctctta atacggataa
    26281 attgcagcgc gcattatgac aaatattact tgtcgatgca gtaaagatgc gggcgcgatg
    26341 agttcacgcc cggcaggaga ttagttgccc agaccgcgac cggcgtcttt ctcttcgatt
    26401 aactcaattt tgtaaccgtc cggatcttcc acaaacgcga taaccgtagt gccgcctttt
    26461 accggacccg cttcacgggt gacgttaccg ccgttctgac ggattttttc gcacgcttca
    26521 gcggcgttat ctacgctaag cgcgatgtga ccataagcag tgccgagttc gtatttatcc
    26581 acgccccagt tgtaggtcag ttcaatcacc gcttcttcgg tttccgggcc gtagccaaca
    26641 aacgccagtg agtatttgta ttccgggttt tcgctggtac gcagcagttt catgcccagt
    26701 actttggtat aaaaatcgat ggagcgttgc agatcgccaa cgcgcagcat ggtatgaaga
    26761 agacgcatct ttatatcctc aaaatggcta acagattcaa aatgttgttt tagtatagcg
    26821 gcggctttgc gccgctcgca atgttggatt acagcgtcgg gtaatcagtg tagccttccg
    26881 cgccgccacc gtaaaaactt tcggcacgct gtgggttaag ctcagctttg cgttgcaagc
    26941 gggcgaccag atccgggttc gcaatccagt cacgaccaaa tgccaccgca tcaattaacc
    27001 ctttgccgat cagcgtttcg gctttttcta ccgtgtatgc acctgcgccg ataatcggac
    27061 cgtggaaacg ggcgcgtact ttttcgcgga acgcatcagt atacggttca ccccccgccc
    27121 aatctggttc tgacatatgc agataagcaa tgccgcgttt acccagttgt tctatcagat
    27181 acagtgcatc ggcttcttca ttcgggccgt tatctgtgtt ctggaaagta ccgattggcg
    27241 aaacgcgaat gccaatgcga tcggcacccc attcttcaat cccggcatcg accacttcca
    27301 gcaccagacg tgcgcgattt tccacgctgc cgccgtactg atcggtacga tggtttgaag
    27361 aaggagaaag gaactgatgc agcaaataac catgagcaga gtggagctct accagatcaa
    27421 aaccggcttc acgcgcgtta gcaatggcct gacggaaatc attgacgata cccggaatct
    27481 cttccagttc aagcgcacgc ggcatggatg tttcaacacg gatcgcctga ccattttcat
    27541 cgcgcagaga agtacgtgtt cccgcgctaa gtgcggaagg cgctaccggt gcctgaccgc
    27601 caggttgcag gctggcgtga gaaatgcgtc cggtgtgcca cagctgtacg gccatatgac
    27661 cattttcagc atgaacgcca gcggtaattt ttttccatgc ggcaatttgt tccggactat
    27721 ggatgccagg tgcaccagca tacccttttg cctgggcaga aatttgcgtg gcttcgctaa
    27781 taatcaaacc ggcactggca cgttggcgat agtattccgc catcaacggg gtagggatgt
    27841 cacccggttc aatactgcgc agacgcgtca gcggtgccat aaaaatacgg tttgccgccg
    27901 tgatcgcgcc cactttcagt ggggaataca gtttttcaga tgacataaag gctcccggtc
    27961 gtctggtgat aaaaaatgcc tgctaaacgg caggcgtcgc aataatggtt tttacatgtg
    28021 ccagcgcgtt ttccagtggc tcgaaactgc gcgaaatttt ggcctgcaga ttcgcaccca
    28081 gccatagtgc gtaaagcact tgtgcctgtt gcagcggttc gccacaaaag gttaaacaat
    28141 ggctatcacg gccattttcc agcgcctgcg agagcagggc gatcacgccg cgagcgcctt
    28201 tatccatcgc gctacgcata tcttctgaca gatcgcacac ttcggcagag agttttactg
    28261 tcaggcaacc actgatggtt ccatgttggc aaaactggtt cagtgtttgc tggtaataag
    28321 ccagtatgcg gtcgcggtag ttaccttcgc cggattgcag caactcagtc agtcgttggt
    28381 gatatgtggc gtaatggcgc tcaagcatgg caacgccaaa cgcttcttta gagcgaaagt
    28441 agtgatagaa ggaccctttc ggcacttcag cggtttttag taattcgctt agccccatcc
    28501 cggtgaatcc acgttgcagg caaagttgct cgcccgtcgc caggagatgt tcgcgagtat
    28561 catgttcggt gtgtttgttc atgcgttgga gtgtagtaga ccagtcggtc tagtgcaagc
    28621 aaagttgcta ctttgggcat taacagcttc aataatggta ataatcttat ttgaggaggg
    28681 cgaagtggcg gagcaattag agttctttcc cgttcagagc ccgtgccggg gaatttgcca
    28741 gtctgacgaa cgcgggtttt gtcgcggctg ttttcgtagt cgggatgagc gttttaactg
    28801 gaataaaatg agcgatggcg aaaaacaaga ggtgctgcgc ctttgtcggc agcgattaat
    28861 gcgcaaatta cgcgcaaata agccagcgtc atcagacgaa ccggagcaac catcactctt
    28921 ttgagcctaa tcttgcgtat actcctctca tatctgataa gaggaagcga ttatggttca
    28981 gcgtattact attgcgccgc aaggcccgga gttttcccgt tttgtgatgg gctactggcg
    29041 attgatggac tggaatatgt ccgcccgcca gctggtcagt tttattgaag agcatctgga
    29101 tctcggcgtg accaccgtgg accatgctga tatttatggt ggctatcagt gcgaagcggc
    29161 gtttggcgag gcgctgaaac tggctcctca ccttcgtgaa cggatggaaa tcgtcagtaa
    29221 atgcggtatc gcgacgaccg cgcgtgaaga aaacgtcatt ggtcattaca ttactgaccg
    29281 cgatcacatc attaagagcg ccgaacagtc gctaattaat cttgcgaccg attatctgga
    29341 tctgctgtta atccaccgac cagacccgtt aatggatgcc gatgaagtgg cggacgcgtt
    29401 taaacatctg catcagagcg gcaaagtgcg ccattttggc gtatcgaact ttacgcctgc
    29461 gcaatttgcc ctgttgcaat cacgtctgcc gtttaccctt gccactaatc aggtggaaat
    29521 atccccggtg catcagccgt tactgctgga tggcacgctc gaccaactgc aacaactgcg
    29581 tgttcgtccg atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg atgattattt
    29641 ccagccgctg cgtgatgaac tggctgtggt ggcggaggag ttaaacgcgg gctcgattga
    29701 acaggtggtt tacgcctggg tattacgttt accgtcgcag ccgctgccaa ttatcggctc
    29761 aggtaaaatt gagcgtgtac gggcagctgt cgaagctgaa acactgaaaa tgacccgtca
    29821 acaatggttc cgtatccgta aagcggcatt gggttacgac gtaccgtaag cacttttagg
    29881 aatagccgcc gttcaaaaat gtgtcactgg tttacactta ttcaggaatg cacaatgaac
    29941 ggaggtccta tgaaacgttt tagtctggcc attctggcgc tggttgttgc gaccggcgca
    30001 caagctgcca gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt tgggcagtca
    30061 attggtagcg tcaccattac tgaaaccgat aaaggtctgg agttttcgcc cgatctgaaa
    30121 gcattacccc ccggtgaaca tggcttccat attcatgcca aaggaagctg ccagccagcc
    30181 accaaagatg gcaaagccag cgccgcggaa tccgcaggcg ggcatcttga tccacaaaat
    30241 accggtaaac atgaagggcc agaaggtgcc gggcatttag gcgatctgcc tgcactggtc
    30301 gtcaataatg acggcaaagc taccgatgcc gtcatcgcac ctcgtctgaa atcactggat
    30361 gaagtcaaag acaaagcact gatggtccac gttggcggcg ataatatgtc cgatcaacct
    30421 aaaccgctgg gcggtggcgg tgaacgctat gcctgtggtg taattaagta attaagaggc
    30481 cagcgtacct tgtggcggtg cttgctcaag ttgcgaaagc gagcagtaca gccgccagac
    30541 aattgccgcc agctcgcggg cggcaggttg atgatggcgg gcaaggctgt cgcaaatccg
    30601 ctgtaattct tcaagtgtgg ccgccagtga tttttgctga acgccacgct cactcatcac
    30661 tccccgcaac agtgaaatac agttatcccg tactcgcgat aacggatcgg aacgcgattc
    30721 ccagtcgcgc aattgccaga caacatgaga acagttcagc agcactacac cccagcgtaa
    30781 taaccaacgg cgagccagcg catcctggct gttactcaac tgactgacgt gatgataagt
    30841 gagcgattca aattcgcttt cactcagtgt tggatggcgg cttagttgat cgacaaaatc
    30901 ccggcgcagc gcgcgtatat ggcggcggct tttgcgagca tccgatcctg gacgcagaat
    30961 ggcgaacgct aaccacgcca acgcgacgcc aacgattttt gccagattat cgttaagaaa
    31021 atcagcaaaa tcatacaccg ggggattagt gacagcgata aacgaaccca taaaaacaat
    31081 cagttgcccc cacagtgcgg caaatttggg catctgcaat ttaagaagct gcattgtcgc
    31141 cagcagtgga aagagaaaca gtaaaaattg ccacagatcg ctaatctgca ccatcaggcc
    31201 aaatttgacc acaaagctga atagcgaaag taacaccagc gtgcgcatca gcagtgacaa
    31261 cgacttaaac ggtgctgcga cggcggagta gagtacgcag ctaattgctg ccagcgttaa
    31321 tgcattggaa ccggcatccc attgcgaagc aatactccat gcgccaatca tcatcaacgt
    31381 acaaaatgta cgcagtccgc tccacatagc ttcggcgtta tcggtatgac gagccaggcc
    31441 ggatgtccgt gggagtctgg tatgatcatc cacaccgctt tgcagatgtt gtaattcctg
    31501 actactttgc agataaaggc ggcaaaaata acgtagtcgc tgccagaagg cgacgtgccg
    31561 atagtcggcg acgttggtcg ggcgtagcgg ggcgataata cgtgcgacgg tgtaaacatc
    31621 tgtttgcgaa ctggcgagcg ccgtcagcag ctgttcgaga atttctcgtg tggcacatgg
    31681 cggtgagggc cagttgagca acatacgtcg caggctggag atgacactgg tcatacgtaa
    31741 ttgctggtgg agcagcgcat taaggcgtgc gttttgctgg cgaaaacgat agtggctcca
    31801 gaaagcctgg atacggagca aattcatggt cagtatctgt ccaatcaccc cctcatgtgc
    31861 tgcacgaatg gcatcggttg tttcaggctg ccagagtaaa ctggcatgtt ccagtaatcg
    31921 ggcgtgcatg tttttcaatg cggttaaaag ggctgtagca tcggaactgc tcggcaggat
    31981 catcatcatc atgccgccgc acaaaatacc gacaatcacc tcgcaaacgc gcgcctgagc
    32041 gatatcccac agctggctgg cctcagtaat attaaccatc ggaaaggcga tgatggcagc
    32101 tgtgtagccc gccagttgaa atgcatacgc gacattattc gtgaagtgcg cacaggccca
    32161 ggtacaaaag ccaagccacg ccgccatgct caaaagaaaa aaccacggct cattgagcgt
    32221 atgtccggca agaagcagtg ccgcaatggc tccgagcaaa ctgccagcga tgcgtccgag
    32281 gcttttgctg atgacaccgc caacggtggg aaagctaacc acggcagccg aggtcatcgc
    32341 ccaatagggt tcatccagat ttaaataata ggcaaccgtc agcgccagac acatggcaat
    32401 ggtattgcgt aacgcataac gccattgcgc cagcgtggcc ctgaaccagg gcaaattgcg
    32461 caaggaccat gacgatgcgt tcattattga cctacggcaa tactacaggt ggtgcccgac
    32521 accagcgtga catcgcctgg gactttatcc agcgcaaagc ggacgggaac tcgttgggcg
    32581 aggcgaaccc aggggacgtt aggtttcaca tccggaataa ggtttgagtc actttcaacg
    32641 ctctgatcat aaatcgcccg accgatactg gaaacgtgac cctgtaacgt tttgttatca
    32701 ctatagaggg taatttgtgc tggtgcacct tcgcggatat ggcgcaactt ggtttcttca
    32761 aaataaccaa tgacgtaaaa cgagtggctg tcgacaaggg caaacagcgg tttcccggtg
    32821 tcagcgtaat cgccgatgcg ggtagtgagg ttagtcaccc atccactcac cggggcgcga
    32881 atttccgttt gcgccagttg ccattgtgcc tgtttcagcg tcgcctgtgc ggcatcaaca
    32941 ctggcctgca tcgctttaac attgaggtta gcggtatcga gttcttcggc agaaataaaa
    33001 ttttgtgaga gatgacgacg acgattcgct tcgttattgg cttttgcgag gtcagattgc
    33061 gctttcgcca actgagcctg tgcgttcagc tcggcgatct gaaagggcgt tttatcgatc
    33121 gtgagcagaa gatcccccgc gttaaccagt tggttgtctt ttatattcag ctcaacaata
    33181 cgtcccgaca cctgaggggt gatagaaacc tgttctgcgc gtattttccc gtcgcgtgtc
    33241 cacggagatt gcatgtaata attccacagc caccatccgg caagaaccgc aactaccgct
    33301 acaatgattg ttgagaaata cttaattgtt ttaattgaca tagttaccac gcaatcagta
    33361 ttgccaggga aaggcaaacg caaatcgcaa acagcgataa atccattaac aagggatgcc
    33421 agatgtcacc ggcgtagatc cagccacgaa gcaggcggtg tacgacaagc cagatgacga
    33481 atccaaacgc gaaggctttg aaaaacggag gaaagtagac ggacgcaccg aacaccagat
    33541 cttgtaaggg caatcctgtt gcattgagca taaacttcac aaagtagatt cctttacgac
    33601 cgaatatatt gcgttagatt aataaatatt ctttaagtgc gaaaaattta cgcgcaattt
    33661 ctgaaaaata tgtctgacgg taaccaaatg cagcaataca tttgttttag caatacaatt
    33721 gctgcacact attctaaaag ccgcataata tcttagcaag ctaattataa ggagatgaaa
    33781 ttggaatcgc cactaggttc tgatctggca cggttggtgc gcatatggcg tgctctgata
    33841 gaccatcgcc tgaaaccgct ggagttaaca caaacccatt gggttacgtt acacaatatc
    33901 catcagttac ctccagacca gtcgcaaatt caactggcaa aagcgattgg catcgagcag
    33961 ccatcactgg tccgtactct ggaccaactg gaagaaaaag ggttaatttc gcgtcaaact
    34021 tgtgccagcg atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga gccgctgatc
    34081 agcgaaatgg aagctgttat taacaaaacc cgcgcggaaa ttttacatgg catctccgca
    34141 gaggaactgg aacaactgat taagctcatc gcaaaacttg agcataatat cattgagtta
    34201 caggccaaag ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca cgcaaactta
    34261 tttagcgcgg ggaaacggtc acctgactgc cattgctggc cagtacgaca cgttggcccg
    34321 gagagaaacg agtgttgcct tgtttctgta ccaccatgat ggtattacca tcgtctttac
    34381 gaatttccag ctcgacaccc tgcgttttgt tcattgcact ctgtacgccc tgaccggcta
    34441 cgccacctgc aacagcgcct gcagcagtag ccagagaacg cccggttccg ccaccaacag
    34501 tattccccag gaaaccacca agaacagcac cgccaattgc gccgataacg ttggaatcat
    34561 caccgccctg aatctgtacc ggacgtacgt taacgatggt gccatagctg acattctgta
    34621 cttgtttcgc ttcagaagcg gtataaacat cccctgacag ggtgtcgtta ttaacacaac
    34681 cgacaagaga cagacctacc attgaaacaa ccaatacgcg tttaatcatt gaaaaatctc
    34741 ctgttcacca tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg gcatatgtga
    34801 atccatggtt acatgttcta ccaaatatga gcaaatcata atgaaagtag gatcaaccaa
    34861 ttataaacaa aacattcatg atgattattg atttgaagat aatgttttac taccaacggt
    34921 aaaaatcggt cattccgcat ggtattcacg aaaagtttat gtttgagtgg tgcgcaaacg
    34981 tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac
    35041 cagccttgat ggtgttgatg ttgtgttggc gacaattgat gaacaccggg tcgcacagct
    35101 ggcaagtttg agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca
    35161 gggccagcag cttatacttt ctcagtttgg acagcttgat actcaactcg ggcgactttt
    35221 tgctgatgcg gtcaatgcct tgcttaagga acaaaacctg caggcgagag atatcgttgc
    35281 gatcggctgt cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac cacacacttt
    35341 acagattggc gataacaatc aaattgtggc acgcaccgga attacggttg tcggtgattt
    35401 tcgccgtcgc gatattgcct tgggaggaca aggcgcaccg ctggtacctg cgttccatca
    35461 tgcgctgctg gctcacccaa ccgagcgacg aatggtgctc aatattggcg gcatcgccaa
    35521 tctgtcactg ctcattcctg ggcagccggt tgggggctac gataccggtc ctggtaacat
    35581 gctgatggat gcctggatct ggcgtcaggc cggtaaacct tacgataaag atgccgagtg
    35641 ggcacgggcg ggtaaagtta ttctcccact gctgcaaaat atgctcagcg acccgtattt
    35701 ctcgcaaccc gcaccgaaaa gcaccggacg cgaatacttt aactacggct ggctggagcg
    35761 ccatttgcgc cattttccgg gtgttgatcc ccgtgatgtg caggccacac tggcagaact
    35821 caccgccgtg accatttctg aacaagtttt gttgagcggt ggctgtgaac gattgatggt
    35881 atgtggtgga ggaagtcgta atccgctact catggcgcgt ctggcggcat tactgccagg
    35941 cacagaagtc accaccactg atgccgttgg cattagtggc gatgacatgg aagcattggc
    36001 tttcgcctgg cttgcctggc ggacgcttgc gggattacca ggaaatctgc cttccgtcac
    36061 tggcgcaagc caggagacgg tactcggggc tattttcccc gctaacccgt gatatcagag
    36121 ttaactgaat ttttcttacc gcgtctgccg ttacacttgg aacgttaggg agggcgtatt
    36181 gccctccaga ccaggaaagt cttcgggata tgtctatgaa aaaactgtta ctgatctgtt
    36241 tgccggtcct tctcaccggc tgtagcgcgt tcaatcaact gtttgagcgc atgcaaaccg
    36301 atacgctgga ataccagtgt gatgaaaaac cgttgacggt caaactgaat aatccgcgcc
    36361 aggaagtcag ttttgtttac gataatcaac tactgcatct caaacagggc atttctgcgt
    36421 ctggtgcacg ttacaccgac gggatctatg ttttctggtc gaaaggcgag gaagcgactg
    36481 tctataaacg cgaccgcatc gtcttgaata actgtcagtt acaaaatcca cagcgttgag
    36541 atttttccag gggcggcgca caatagcgtc acccactgac aatccgtaaa gaaaaccatg
    36601 tctgataacg acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcgga
    36661 ttacgccgcc gcgatcttcc cgccgatcca ttaacccttt ttgaacgctg gctctctcag
    36721 gcgtgtgaag ccaaactggc ggaccctacc gcgatggtgg tcgctaccgt ggatgaacat
    36781 ggtcagcctt atcagcgcat cgttttactc aaacattacg acgaaaaagg catggtgttt
    36841 tacaccaacc tcggcagccg taaagcacat caaatcgaaa ataatccgcg cgttagcctg
    36901 ctgttcccgt ggcataccct tgagcgccag gtgatggtga tcggtaaagc ggaacgactt
    36961 tcgactctcg aagtgatgaa atattttcat agtcgcccac gtgatagcca gattggtgca
    37021 tgggtttcga agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg
    37081 gagctgaagc agaagtttca acagggcgaa gtgccattgc cgagtttttg gggcggtttt
    37141 cgcgtcagcc ttgaacagat tgagttctgg cagggcggtg agcatcgcct gcatgaccgc
    37201 tttttgtacc agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat
    37261 gcaaaaatct tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctatt
    37321 tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt
    37381 tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga
    37441 cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct
    37501 tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac
    37561 gcttccagca ggcgggccac aagccggttg cgctggtagg cggcgcgacg ggtctgattg
    37621 gcgacccgag cttcaaagct gctgagcgta agctgaacac cgaagaaact gttcaggaat
    37681 gggtggacaa aatccgcaag caggttgccc cgttcctcga tttcgactgt ggagaaaact
    37741 ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc
    37801 gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc
    37861 gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg
    37921 gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt ggtggttctg
    37981 accagtgggg taacatcact tctggtatcg acctgacccg tcgtctgcat cagaatcagg
    38041 tgtttggcct gaccgttccg ctgatcacca aagcagacgg caccaaattt ggtaaaactg
    38101 aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct
    38161 ggatcaacac agcggatgcc gacgtttacc gcttcctgaa gttcttcacc ttcatgagca
    38221 ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc
    38281 agtatgtgct tgcagaacag gtgacacgtc tggtgcatgg tgaagaaggt ttgcaggcgg
    38341 caaaacgtat taccgaatgc ctgttcagcg gttccttgag tgcgctgagt gaagcggact
    38401 tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggagaagggc gcagacctga
    38461 tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg
    38521 cctccaatgc catcaccatt aacggtgaaa aacagtccga tcctgaatac ttctttaaag
    38581 aagaagatcg tctgtttggt cgttttacct tattgcgtcg cggcaaaaag aattactgtc
    38641 tgatttgctg gaaataatgc attaagtgga aagggggagt gagaaatcac tccccctggt
    38701 ttttatacag ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt
    38761 catgcgggta acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa cgtctggccg
    38821 ctgaacaccg ttcaattttc taatcacact caatacggca aatggactgg ctgcgtgatg
    38881 ccgcccagcc atttaaccga aattgtgcaa ggcattgccg ccattgataa attacacacc
    38941 tgtgatgccg tattaagtgg ctatctgggt tcggcggagc agggtgaaca tattctcggt
    39001 atcgttcgtc aggtgaaagc agcgaatccg caggcgaaat atttttgcga tccggtaatg
    39061 ggtcatccgg aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac
    39121 ggtttgcctg ccagcgatat cattgcgccg aatctggttg agctggaaat actctgtgag
    39181 catgcggtaa ataacgtcga agaagcggtt ctggcagcgc gtgaactcat tgcgcaaggg
    39241 ccacaaattg tgttggttaa acacctggcg cgggctggct acagccgtga ccgttttgaa
    39301 atgctgctgg ttaccgccga tgaagcctgg catatcagcc gtccgctggt ggattttggt
    39361 atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt tactgctggt gaaactgctt
    39421 cagggggcaa cgctgcagga ggctctggaa catgtgaccg ctgcagtata cgaaatcatg
    39481 gtgaccacca aagcaatgca ggaatatgag ctgcaagtgg ttgctgctca ggatcgtatt
    39541 gccaaaccag aacattattt cagcgcaaca aagctctgaa atatctcatt tcggcccaca
    39601 acgacctgtg ggccgatggc tattacttta agccttccgc tgacagcgcg tcttgtactt
    39661 ccggacgttc agccatacgt tgcataaatg ccgcaatgtg ctctaaccct tccagattca
    39721 gtttcaccgc gtatgcccag cgcagaaccg taaacagata ggcatcagca attgtaaatc
    39781 gttgcccgca gatccagtgc tcatccttca gtgcctcgtt cacatattgc agcttcttct
    39841 ccagctgcgc gcgaactgtc gatttgtact cttccggtgt atcagggcga aacagaggtg
    39901 tgaaaccttt atgcagctcg gtggcgatgt aattcagcca ttcgatggtt ttatagcggg
    39961 aaatactgtt taccggtgcc agcaactggc ggtcggggac gctgtcggca agatactgca
    40021 taatcgctac gccttccgtc agcaaagtac catcgtccag cagcaatgca ggtacctgtc
    40081 ccttagggtt aacggcaaag taatcgtcac cgttttcgag acgttttttc attaaatcca
    40141 cactgacgag cgtaaaatcc tttccgctct cacgcagggt gatatgggaa gcgagagagc
    40201 aggcacccgg tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat
    40261 ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc ttgtaaaaga
    40321 gtttccctga attttacgct acggctgctt tcgccgcttt gtctgcagcg tcatcctgcg
    40381 tcatgcggtg cagtttcggc gcggtcagca gcatcagtac tgcaataacg gcagtagcga
    40441 caccaatctg caagaatacg cgaccataga cttccagtga catcagtgga tcggtaacgt
    40501 tatccggcac agccatcata cccgcaacat aaccaccaat caggtttgca ccggcagtgg
    40561 tcaggaacca gctacccata atgaagccca tcagacgctg cggaacgagt tgtgcaacca
    40621 ttgccagacc cagaccagag atcatcagtt ctccgatact ctgcaggcca tagcttgcga
    40681 ccagccagct tacagacacg atacctgcgt cagatgcgaa ttttgcaccc agcggcagaa
    40741 tcaggaacgc accagaacac atcaccatac cgattgcaaa cttagttggc atcggcaggg
    40801 tatcgcccat cttgttatag atagcggcca gaatcgggct accaatgatg atccagaacg
    40861 ggttcagtgc ctgatactgc tcaggttcta cggccaggcc cagaatggtg tgttcaacgt
    40921 tacgaatcgc aaagaagttc agtgacgttg gcatctggct gtacagcacg aagaagataa
    40981 tggcttcgag catcaggatg aaggcaacga tcattttacg acgcgcagca cctttcatcg
    41041 cgaaggcttc tttaccgaag ataaccacga taccaaaggc aacaacgccc agcgccatac
    41101 gcgcaacttc ctgattgtgc agcagccagg tggcgatagc gatcagtgcc acaacaccaa
    41161 taatggtcag cagcaggtta cggtagttga taggctcgaa gtctggtttt gaaccgtact
    41221 gtttaaccca gcgttggcag aaggcgaagt taacgatagt gatcagcagg cctacaacgc
    41281 tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag ccacggggtg gcgatcatag
    41341 agaagaaaga gccgatgttg acggacatgt agtacatggt gaatgcaccg tccagacgcg
    41401 ggtcgttttt ctcatagcat gtagaaagca gagaagacgg gttagcttta aacaggccgt
    41461 taccgaccgc aatagccgcc atacccatat aaacgatacc ggcgtcgtga ccagaccagg
    41521 caaccagagc ataaccaatc gccagcacaa tagcgccaag cataattacg cgtttagtac
    41581 ccagtacctt gtcacctaac cagccgccga tagcgaccag accataaacc agggcactaa
    41641 aggaagagaa aagggtgatt gaatccgctt cagacatacc cagttgttta accaggtaaa
    41701 cagccataat tccttgtagg ccgtaataac caaaacgttc ccataactca atcgagaaga
    41761 tgagatagaa cgccttcggt tgtttgaaag cgttcaaact gacgctttca gttggttttt
    41821 ggtttgcagt ggacactttt acctcttttt ttacatccca tattaacggg ggtgctttct
    41881 cgcgttatgc ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa cggcaggtaa
    41941 tgttcactat cttgcacttt cagacaagtc ctttgtaata atctgttaca tataactgag
    42001 gcggggaatt caaccattcc gggaaatgtt atccagcgtt aaatttttca aaaaacgtaa
    42061 cggcagattt tttcactacc ctgagagggg gatgatggct taacggcgat atgttctgct
    42121 atttagcgtt tttcctagtg agatagtcca gtttctgaaa aatagccagt gtaatgtttt
    42181 gtaggtcaat atttgttgcg tgtgtaaatt taaatgattt taacctagtt cttgcaatgt
    42241 ttttacattg ccacagcatc gtgatcttga tcacgaatga atagaaaatt tcgctattaa
    42301 aaaaacaatt aacctgcatt tttggttatc cgcaaacgga ttacctgatg ataacgctaa
    42361 gcaatggcat cattgacgtt aaaaagatgt tttaaaagag gataaagaaa ggttatcaat
    42421 ggggtaatcg gtgttacccc ttttcttcag atgtcaactt tctctttgta ttcacaaaga
    42481 tcttcaataa tacaagagcc acagcgaggc ttgcgggcaa tacaggtata acgcccgtgc
    42541 aggatcaacc aatggtggca gtcgacttta aactctgccg gaaccacttt cagtagcttt
    42601 tcttctacct gttcgacgtt tttccccggc gcaaattgag tacgattaca aacgcggaaa
    42661 atgtgcgtgt cgacagcaat agtaggccag ccgaatgcag tgtttaatac gacgttggct
    42721 gttttacgac ctacaccagg cagggcttca agcgcagcac gatcttccgg aacctcgcca
    42781 ttatgctgtt ccagtaagat acggcaggtt ttgatgatat tttctgcttt gctgttataa
    42841 agcccaatgg ttttgatata ggttttcacc ccttcgacgc ccagttcaag catcgctgca
    42901 ggtgtattag ccaccgggta gagtttcgcc gtcgccttat taacactgac atcggtcgcc
    42961 tgagcggaaa gcagtacggc aatcagcaat tcaaaaggcg aactgaaatt aagctcggtg
    43021 gtgggatgag gattgttctc acgcaggcga gtgaggatct ccaggcgttt tgctttgttc
    43081 atcagacatt ccctgtttca ccgtttggca atgcacgttc ggcagctgct tcagcacggc
    43141 gctttttcat tctttcatca atcaggtatt tccctgccag catcagtccc aggccaataa
    43201 atgcacctgg tggcagcatc gccagcagga aaggggagtc ggtgtggaat atctccacgc
    43261 gtaatacttt tgcccagcta cctaacagcg catctgcacc gtcaaacaat gtgccattgc
    43321 cgataatttc gcgtagtgaa cccagcacga acatggcgca ggttgcgccc ataccaattg
    43381 aaaagccgtc cagtgccgaa agcgccggac cttttttggc ggcgaaggct tcagcgcggc
    43441 ccacaacgat acagttagtg acaatcagcg gaataaaaat ccctaatgat tgatacaggc
    43501 caaaggcgta ggcgttgatc agcatctgta cagcgctgac caccgaggcg atgatcatca
    43561 cgtaaatggg aatgcggatc tcggctggcg tccagtgacg cagcgtcgaa atggtcaagt
    43621 tggtcagcgt cagtaccagc gtagtcgcaa gtcctaaacc cagagcgtta gtggcagtgg
    43681 acgtgaccgc caatagcgga cagaggccga gcaactggac cagcgcagag ttgtttttcc
    43741 acaacccctg aacaataacg tctttaattt cgctcatgct ttattctcca caggcaggaa
    43801 gttgagaaag ttgtgccggt aacgtctgag cgtacaatcc ggcgcgtttt accgcattta
    43861 ccaccgcgcg gggagtaatc gtcgcgccgg tgaactggtc gaaatcacca ccatctttct
    43921 tcaccgccca gtttgcatca tccgcaccac tgattttttt acccgcaaaa tgggtgatcc
    43981 agtcagaaag gcgcagttcg attttatcgc caagccctgg cgtttcgtgg tgctctgtca
    44041 cgcgcgtgcc aagtaccgtg ccgttaaaat cggctcccac cagcagctga atcgcaccgg
    44101 aatagccatc tggcgcggtt gtttccagaa cggcggctac cggtttgtca tcctgtttgg
    44161 cgatgtaaac ccgatgctca cctttaccta actctggcgc agtgaccaga tagcaactct
    44221 gtgccagcgc attgttatag cgttcggctg gcagcacctg atcaaataac gccttttgtt
    44281 gcagactggc ctgttcagca attgtcgttt tggtcatctg gttgatggcc gcagttaacc
    44341 ctgtagaacc cgctgcaaat agtgccagtg taatgccgtg ttttcggata gttttcagca
    44401 tggtttaccc tttgcgatgg ccgtagacgc gcggacgcgt gtagtaatcg atcagaggaa
    44461 ccgtgatgtt cgccagcagg acggcaaaag ccacgccgtc aggatagccg ccgaaactgc
    44521 ggatcaacca gactaataaa cccgccagcg cgccgaacat cagacgacca cgattggtcg
    44581 tagaagcagt aaccgggtca gtcaaaataa agaatgcgcc gagcatagtc gcaccagaca
    44641 gcagatgaat ttgcggtgat gccagtgttt ctggtgagaa caaccagccc aacgttgcgc
    44701 ataacgccag cgttactaag aagctgaggg gaatatgcca gcgaatcgct ttctgccata
    44761 gcaaccacac gccgccagcc agccaggcga gatttaccca ttgccaacca acgcccgcca
    44821 gcataccgct gtagatcgga tattgcataa tctgttcaac cgaatgaccg gcacggacag
    44881 aggttttaaa tgtatccaac ggtgtcgcct gactaatgcc atcaatacct aagcgtagcg
    44941 tgttcatatc accaccactg gtggtatgtc cgctgaaaat aacctgtatg gcgtcgataa
    45001 aaccagggat gttgaccgca atttcatgtg gcggtaacca gctggtcatc tgcaccggga
    45061 aggagatcag taagaccaca taaccaatca ttgccggatt aaacgggttt tgccccagac
    45121 cgccatacaa ctgtttagca atgatcaccg caaacaccgt acccagcacg accatccacc
    45181 atggcgcgag ggggggaata cttaccgcca gcaataagcc tgtcagcaat gctgagttat
    45241 ctttcaacgt tgcggctacc gactgcttgc gtagtttgag tacgagagct tcggctaaca
    45301 gagcactgac cgacgccaac aggatctgaa cgagagtacc ccaaccaaaa aaccacagtt
    45361 gcgctgcgat tcctggcacg gctgcgagca acaccaacag cataatgcgc gatgtttggc
    45421 gctggttatg ggtataaggg gagctagcta ttctgaatac catttagtcc tcgtttacaa
    45481 ccttttgctg ggcggctttt ttggcctgaa cgcgggcaat agccgcagca actgccgctt
    45541 tgcgcggatc aatctgttct tctggctccg cattagcctg ttgctgttcc agcttgcgtg
    45601 ctttggcacg ggcaatagca gcttcgacgg cggctttgcg cggatcaacc tgttcttctg
    45661 gttctgcgtt agcctgttgc tgttccagct tgcgcgcttt ggcacgggca atagccgctt
    45721 cgacggcagc tttgcgcgga tcgacctgtt cttctggttc cgcattagcc tgttgctgtt
    45781 ccagcttgca tgctttggca cgggcaatag cggcttcgac ggcggctttg cgcggatcga
    45841 cctgttgttc tggctcggca ttagcctgtt gctgctccag cttgcgcgct ttggcacggg
    45901 caatagcggc ttcgacggcg gctttgcgcg gatcgacctg ttcttctggt tccgcattag
    45961 cctgttgctg ttccagcttg cgcgctttgg cgcgggcgat agcggcttca acggcagttt
    46021 tacgtggatc agcaacggtt gctgcgtcgt tagtttgctg cagttctgcc tgtttcgctc
    46081 tggcttgcgc tttacgggct tcccgtgctg caataattgc actgttatcc gggcgttcgc
    46141 ccgctttaat cacaataggc tgtgtagcct gggcctgttt ctctttcacc cgcgccagag
    46201 cggcagcaat cgcatcttta tctttggctg caggttgaac ggctgcgctc ttatgtcgtt
    46261 caaggcgagc cgctttttcg cgctccagac gagcctggcg cgcttcgaaa cgcgctttgg
    46321 cttctgcggc gcgcttttct tcctgacgaa tagctgcaat ttcagctttt tcctgacgga
    46381 aatattgcac caggggaata ttgctcgggc aaacccacgc gcaagcccca cattcaatgc
    46441 agtcggcaat gttatgcgtg gtagctttat cgtgttgctg acctttgctg aaccagtaca
    46501 actgttgcgg caaaagatcc gcagggcagg cgtcagcaca ggcgctacac cggatgcagc
    46561 tttgttcttc ctgtggttcg ccaagctcat tggcagaggg agccaacaga cagttggtaa
    46621 tctttacgac cgggacatcc agccatggca aggtaaagcc cattagcggg ccgcccataa
    46681 tcaccatttg atcggcagag gggcagaatc cggcgtcatt caataaatga cgcactggcg
    46741 tccccagccg tgcccagacg ttgcccgggc gagcgattgc ttcgccagtc agggttacga
    46801 cacgctcggt aatcggctcg ccatcaataa cggcacgttt cactgcataa gcagtgccga
    46861 cgttttgcat taatacgccg atatcggatg aacgcccgcc atgtggaacc tgcttcccgg
    46921 tcagaatgta ggttaattgt ttagcaccgc cagaaggata tttggttgga atcacccgca
    46981 gagaaatatc gtgagagtcc gccagcaccg cgcgcagcat ggaaatcgcc tgcggtttgt
    47041 tatcttcaat gccgataaga atttcgcgtg gctgcagaat atgcgcaaga atgcgaatac
    47101 cctctacgac ctgagccgcg caatcctgca tcaaacggtc atcggcggta atgtacggct
    47161 cgcactcagc agcgttgata atcaacgttt caatcttatc tccgccaccc tgcaatttaa
    47221 cgcctgtcgg gaatcctgca ccgcccagcc cggcaacacc aaactgatga atgcgctcga
    47281 ttaactcttc gcgactgcga gagcgataat cggcccagcc gtctcgcggg atccagcagt
    47341 cttcaccatc ggcatcaata atcacgctta attcagctaa cgctgaagga tgagccgtag
    47401 agtggggcgc aatagccgta acggtacccg aggtgggcgc gtgaacaggc agcattttgc
    47461 cgcgaccacg ggtaagcggc tggccgcgca atactttatc gccgacgcta acgcacaact
    47521 caccttcagc gccaatatgc tgtttcagtg gaataacaaa acgctgcgcc agtggtacct
    47581 ggcgcagggg tgtaccgttg gactgggttt tcatctccgg tggatggatg ccgccgttga
    47641 aatcccagat tttatttttt ctgaatgcag agaataactt aagcatggtg ttccacggga
    47701 atgatacgca cgggaatggt gttcagatcc catttccagg agtcaggtgt ttctgcgacc
    47761 ggttgcaacg agatgcagtg cgtcgggcac ggatcaacac ataaattgca gcccgtacag
    47821 agatcactca ttaccgtatg catggcacgg gtagcgccaa cgatggcgtc taccggacaa
    47881 gcctgaatac atttagtgca gccaatacag ttattttcat caataaccgc caccatccgc
    47941 gcaggcgtta gctcttgcgc ttcgccatcc agcggctgcg gctcgacatt aagcaactcg
    48001 gcaattttta gcatcacagc ttcgccacca ggggcgcaac ggttgatttt ttcaccgtta
    48061 tagctgatgg cttccgcgta ggggcgacag ccgggataac cgcactgacc acactggctc
    48121 tgcggtaaga tttcgtcaat tttctcaacg accggatcgt cttccaccgc aaaacggcgg
    48181 gaggcataac ccagaatggc gccaaacgcc aggcccagca ggctcacggc ggcaacggca
    48241 atccagatag cattcattac aacttcacca aaccactaaa gcccataaag gccagagaca
    48301 taagacctgc ggtaattaat gcaatggcat taccgcgaaa aggtgccggg acatcagcca
    48361 cagcaaggcg ttcgcggatg gcggcgaaga gcaccatcac cagcgagaaa ccgacagcgg
    48421 cggaaaaacc gtacagcgcc gactgaaaga aattgtgccc gagattgata ttcagcaacg
    48481 ccacgccgag cactgcacag ttggtggtga taagcggcaa aaaaatcccc agcagacggt
    48541 aaagcaccgg gctggttttg cgcaccacca tctcggtgaa ctgcacaacc acagcaatca
    48601 ccagaataaa cgccagggtg cgcaggtaaa tcagattaag cgggatcaaa atccacgtat
    48661 cgataagcca ggcgcaaata gacgccagcg tcatcacaaa cgttgtagcc agccccatgc
    48721 ccatcgcggt ttccagcttt ttggaaaccc ccataaacgg acagagaccg agaaacttga
    48781 ccagtacaaa gttattgacc agtacagttc cgacaaacag taacaggtag tcagtcattt
    48841 ttcggcctga aataaaaaag ccgcctatta tccgttaatc cagagcaggc gacaacaggt
    48901 taactgtaag gttattacgg attcacaaac gtcgctttca cccgcgacga gcgtttaaaa
    48961 taggggacga tcaatgcggt tgccaacaaa gtaaacagta actggcgaac ggctatgcca
    49021 tcttccacgg gtgaaaaagc gaacgctttt actgccagca atacggaaat aagcagccag
    49081 ataatatagt gtttaggaac acaacgccga cgtttgaaga aagcgatggt caaccacagt
    49141 gtgtaatacc acatggcgat agcggtaata aaagaaacgc cccataagat ttgtgtcgtc
    49201 agtgcttgtt cgccgagcgt ttgaaatgtt tgcggagaag ataacgcagc ggtatacagc
    49261 aacaacgcca gcgtcgtact taacagagcg actaaaagcc aggccaatgg gccaagcaac
    49321 cagcctccaa tacgttgtgg cgttgtggtg gtcataggtt ctccataata gcggcgcaaa
    49381 caggcaaatc taattttagg ggcgttatta taaggctttt ataaccgatc gctactttct
    49441 ttattggaca tagtgccaga cggacttcgg tacctggccg aggtcaaaca aacgcccgcc
    49501 agaaaccagc tctgcgcgac ggtgatcggc agcacgatac atattgatca gttcctgatc
    49561 gtccgtcagg gtgtaattaa gatggtcgta gagtttttcc aaactttcga gtgaactgat
    49621 tttgcgaaat tttaataagt agtcctgaac agtcataaaa ggtccataaa ataattaata
    49681 ccaaaggggt aggtattatt agtgatgata aaatttcacc aaaagtgaac gcttgtgtta
    49741 ttacaataat aagaatgcgc tgatggcaaa tattcaggga tgaaaaactg ctctgaaaag
    49801 agtaacgcgc tttactatct ctgacaatca ccaacaacat cgaacaagat aataaattcc
    49861 tggtttaata tccgacaagt gaaaacatgc acccggacgg gcagcatgtc gctctacaag
    49921 cgcagagctt acttgtgttg taccgtagta ctctgttcag gtggctgata gttgtcaatg
    49981 tgactcgcca cgccaagaag aatgactgaa acgacaagaa cgatccaacc tgttaattca
    50041 ataagacgat tcattacagc ccacatctct gttgattgat ccattaactt cagggggtaa
    50101 atgttactta gcaataatag ctccagcaag atttttactg aggtttttgc gatatttagc
    50161 ttttgtcgtt ggaaaattcg ctattttttt gacttaagtt aaacaacatc ccttattgct
    50221 ggcaggttat taaactgttg agcgagggta aaggatagtg tcaaatagcc atcatacttc
    50281 aatgagaggc aatgacatga gcgacaacat ccgtgttggg ttgattgggt atggttatgc
    50341 gagcaaaacc ttccatgcgc ccctgattgc gggcacgccc gggctggaac tggcggtaat
    50401 ctccagcagc gatgaaacaa aagtaaaagc cgactggcca acggttacgg ttgtctctga
    50461 gccgaagcat ctgtttaacg atcccaacat agacctgatt gtcattccta cacccaacga
    50521 tacccatttc ccgttagcca aagcggcgct tgaggcgggt aaacatgtgg tcgttgataa
    50581 accctttacc gtgacactgt cacaagcgcg agagctggaa gcgctggcaa aaagcctagg
    50641 gcgtgtgctg tctgtattcc ataaccgtcg ctgggatagc gatttcctga cgctaaaagg
    50701 tttgctcgcg gaaggcgtgc tgggtgaagt tgcttacttt gagtctcatt ttgaccgctt
    50761 ccgtccgcag gttcgcgatc gttggcgtga acagggcggc ccaggcagcg gtatctggta
    50821 cgatttagca ccgcaccttc ttgatcaggc cattacgcta tttggtttac cggtcagcat
    50881 gacggtagat ttggcacagt tacggcccgg agcgcagtcg accgattatt tccacgccat
    50941 cttgtcctat ccgcagcggc gagtcatttt acacggtacc atgctggcag ctgctgagtc
    51001 agcacgttat atcgtgcatg gatcccgagg cagttatgtg aaatatggcc tcgatccaca
    51061 ggaagaacga ctgaaaaatg gcgagcgtct gccgcaggaa ggctggggct acgatatgcg
    51121 tgatggcgta cttacccgcg tggaaggtga ggaacgtatc gaagaaacgc tgttgacagt
    51181 accagggaat tatccggctt actatgcggc tattcgtgat gcgttaaatg gcgatggtga
    51241 aaatccggtt ccggcaagtc aggcaatcca ggtaatggag ttgattgagc tgggcatcga
    51301 atccgccaaa catcgcgcga cgctgtgcct tgcgtgaaaa gaggatggtg caattagcac
    51361 catcc
//
LOCUS       DAZKJR010000016        50191 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKJR010000016 DAZKJR010000000
VERSION     DAZKJR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.016"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1675
                     /locus_tag="ACHOWS_000974"
     CDS             <1..1675
                     /locus_tag="ACHOWS_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFQ0046599.1"
                     /translation="AVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSMDVEPGLYR
                     VTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAVAAEILKRA
                     EASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGETGPQGLQG
                     VKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAATPKAVKAAMDKADGCL
                     EKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFVAWLKEKGAFDQPYWMM
                     KASLPAVLNKVITDVGPGKLNLGGCVIEVMGTYNAAIVRVTIGEYGATGFINGTVCTC
                     TVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDTGSNDETKAATPKAVK
                     AAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGETGATGPAGPRGAQGPKGE
                     KGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGNEQTYRAEESSGFLSISLSIGGVI
                     TGLKSRGADGVISVSYRPLQVTFDGSQWRTVSVGEYVSSGASGFEYFPL"
     gene            1717..2388
                     /locus_tag="ACHOWS_000975"
     CDS             1717..2388
                     /locus_tag="ACHOWS_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFQ0046600.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITGLLTAEK
                     ARGMKGDEINAWRNAMEAANYMFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            complement(2547..2876)
                     /locus_tag="ACHOWS_000976"
     CDS             complement(2547..2876)
                     /locus_tag="ACHOWS_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFQ0046601.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
     gene            complement(3016..3103)
                     /locus_tag="ACHOWS_000977"
     tRNA            complement(3016..3103)
                     /locus_tag="ACHOWS_000977"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3067..3069),aa:Ser,seq:tga)
     gene            3530..4648
                     /gene="hyaA"
                     /locus_tag="ACHOWS_000978"
     CDS             3530..4648
                     /gene="hyaA"
                     /locus_tag="ACHOWS_000978"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFQ0046602.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     KMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            4645..6438
                     /gene="hyaB"
                     /locus_tag="ACHOWS_000979"
     CDS             4645..6438
                     /gene="hyaB"
                     /locus_tag="ACHOWS_000979"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFQ0046603.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLSDDGSELISVQVR"
     gene            6457..7164
                     /gene="hyaC"
                     /locus_tag="ACHOWS_000980"
     CDS             6457..7164
                     /gene="hyaC"
                     /locus_tag="ACHOWS_000980"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFQ0046604.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            7161..7748
                     /gene="hyaD"
                     /locus_tag="ACHOWS_000981"
     CDS             7161..7748
                     /gene="hyaD"
                     /locus_tag="ACHOWS_000981"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFQ0046605.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPAKESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            7745..8143
                     /gene="hyaE"
                     /locus_tag="ACHOWS_000982"
     CDS             7745..8143
                     /gene="hyaE"
                     /locus_tag="ACHOWS_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFQ0046606.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            8140..8997
                     /gene="hyaF"
                     /locus_tag="ACHOWS_000983"
     CDS             8140..8997
                     /gene="hyaF"
                     /locus_tag="ACHOWS_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFQ0046607.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQQQISERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            9131..10675
                     /gene="appC"
                     /locus_tag="ACHOWS_000984"
     CDS             9131..10675
                     /gene="appC"
                     /locus_tag="ACHOWS_000984"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFQ0046608.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            10687..11823
                     /gene="appB"
                     /locus_tag="ACHOWS_000985"
     CDS             10687..11823
                     /gene="appB"
                     /locus_tag="ACHOWS_000985"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFQ0046609.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            11836..11928
                     /gene="cbdX"
                     /locus_tag="ACHOWS_000986"
     CDS             11836..11928
                     /gene="cbdX"
                     /locus_tag="ACHOWS_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFQ0046610.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            12008..13306
                     /gene="appA"
                     /locus_tag="ACHOWS_000987"
     CDS             12008..13306
                     /gene="appA"
                     /locus_tag="ACHOWS_000987"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFQ0046611.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQCLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFSQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(13426..15606)
                     /gene="etk"
                     /locus_tag="ACHOWS_000988"
     CDS             complement(13426..15606)
                     /gene="etk"
                     /locus_tag="ACHOWS_000988"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFQ0046612.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIVSVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEGFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(15626..16072)
                     /gene="etp"
                     /locus_tag="ACHOWS_000989"
                     /gene_synonym="yccY"
     CDS             complement(15626..16072)
                     /gene="etp"
                     /locus_tag="ACHOWS_000989"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFQ0046613.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(16060..17199)
                     /gene="gfcE"
                     /locus_tag="ACHOWS_000990"
     CDS             complement(16060..17199)
                     /gene="gfcE"
                     /locus_tag="ACHOWS_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFQ0046614.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(17245..19341)
                     /gene="gfcD"
                     /locus_tag="ACHOWS_000991"
     CDS             complement(17245..19341)
                     /gene="gfcD"
                     /locus_tag="ACHOWS_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFQ0046615.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(19341..20087)
                     /gene="gfcC"
                     /locus_tag="ACHOWS_000992"
     CDS             complement(19341..20087)
                     /gene="gfcC"
                     /locus_tag="ACHOWS_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFQ0046616.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(20084..20728)
                     /gene="gfcB"
                     /locus_tag="ACHOWS_000993"
     CDS             complement(20084..20728)
                     /gene="gfcB"
                     /locus_tag="ACHOWS_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFQ0046617.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(20835..21140)
                     /locus_tag="ACHOWS_000994"
     CDS             complement(20835..21140)
                     /locus_tag="ACHOWS_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046618.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(21581..21793)
                     /gene="cspH"
                     /locus_tag="ACHOWS_000995"
     CDS             complement(21581..21793)
                     /gene="cspH"
                     /locus_tag="ACHOWS_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFQ0046619.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            22079..22291
                     /gene="cspG"
                     /locus_tag="ACHOWS_000996"
     CDS             22079..22291
                     /gene="cspG"
                     /locus_tag="ACHOWS_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFQ0046620.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            22302..22490
                     /gene="ymcF"
                     /locus_tag="ACHOWS_000997"
     CDS             22302..22490
                     /gene="ymcF"
                     /locus_tag="ACHOWS_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0046621.1"
                     /translation="MTPHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            22465..22695
                     /gene="ymcE"
                     /locus_tag="ACHOWS_000998"
     CDS             22465..22695
                     /gene="ymcE"
                     /locus_tag="ACHOWS_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFQ0046622.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            22685..22858
                     /gene="gnsA"
                     /locus_tag="ACHOWS_000999"
     CDS             22685..22858
                     /gene="gnsA"
                     /locus_tag="ACHOWS_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFQ0046623.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(22906..23979)
                     /gene="yccM"
                     /locus_tag="ACHOWS_001000"
     CDS             complement(22906..23979)
                     /gene="yccM"
                     /locus_tag="ACHOWS_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0046624.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDTPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLNFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(24051..26795)
                     /gene="torS"
                     /locus_tag="ACHOWS_001001"
     CDS             complement(24051..26795)
                     /gene="torS"
                     /locus_tag="ACHOWS_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFQ0046625.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPQRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLLLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDDIDIARANQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            26878..27906
                     /gene="torT"
                     /locus_tag="ACHOWS_001002"
     CDS             26878..27906
                     /gene="torT"
                     /locus_tag="ACHOWS_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFQ0046626.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMAEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQEQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(27879..28571)
                     /gene="torR"
                     /locus_tag="ACHOWS_001003"
     CDS             complement(27879..28571)
                     /gene="torR"
                     /locus_tag="ACHOWS_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFQ0046627.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            28701..29873
                     /gene="torC"
                     /locus_tag="ACHOWS_001004"
     CDS             28701..29873
                     /gene="torC"
                     /locus_tag="ACHOWS_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFQ0046628.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            29873..32419
                     /gene="torA"
                     /locus_tag="ACHOWS_001005"
     CDS             29873..32419
                     /gene="torA"
                     /locus_tag="ACHOWS_001005"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFQ0046629.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGINAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            32416..33015
                     /gene="torD"
                     /locus_tag="ACHOWS_001006"
     CDS             32416..33015
                     /gene="torD"
                     /locus_tag="ACHOWS_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFQ0046630.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATQTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(33270..33575)
                     /gene="cbpM"
                     /locus_tag="ACHOWS_001007"
     CDS             complement(33270..33575)
                     /gene="cbpM"
                     /locus_tag="ACHOWS_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFQ0046631.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(33575..34495)
                     /gene="cbpA"
                     /locus_tag="ACHOWS_001008"
     CDS             complement(33575..34495)
                     /gene="cbpA"
                     /locus_tag="ACHOWS_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFQ0046632.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            34648..34749
                     /locus_tag="ACHOWS_001009"
                     /pseudo
     CDS             34648..34749
                     /locus_tag="ACHOWS_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35030..36271
                     /gene="agp"
                     /locus_tag="ACHOWS_001010"
     CDS             35030..36271
                     /gene="agp"
                     /locus_tag="ACHOWS_001010"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFQ0046633.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(36309..36536)
                     /gene="yccJ"
                     /locus_tag="ACHOWS_001011"
     CDS             complement(36309..36536)
                     /gene="yccJ"
                     /locus_tag="ACHOWS_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFQ0046634.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(36557..37153)
                     /gene="wrbA"
                     /locus_tag="ACHOWS_001012"
     CDS             complement(36557..37153)
                     /gene="wrbA"
                     /locus_tag="ACHOWS_001012"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFQ0046635.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNS"
     gene            37526..37699
                     /gene="ymdF"
                     /locus_tag="ACHOWS_001013"
     CDS             37526..37699
                     /gene="ymdF"
                     /locus_tag="ACHOWS_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFQ0046636.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(37782..39110)
                     /gene="rutG"
                     /locus_tag="ACHOWS_001014"
     CDS             complement(37782..39110)
                     /gene="rutG"
                     /locus_tag="ACHOWS_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFQ0046637.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGHVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(39131..39625)
                     /gene="rutF"
                     /locus_tag="ACHOWS_001015"
     CDS             complement(39131..39625)
                     /gene="rutF"
                     /locus_tag="ACHOWS_001015"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFQ0046638.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(39636..40226)
                     /gene="rutE"
                     /locus_tag="ACHOWS_001016"
     CDS             complement(39636..40226)
                     /gene="rutE"
                     /locus_tag="ACHOWS_001016"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFQ0046639.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(40236..41036)
                     /gene="rutD"
                     /locus_tag="ACHOWS_001017"
     CDS             complement(40236..41036)
                     /gene="rutD"
                     /locus_tag="ACHOWS_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFQ0046640.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(41044..41430)
                     /gene="rutC"
                     /locus_tag="ACHOWS_001018"
     CDS             complement(41044..41430)
                     /gene="rutC"
                     /locus_tag="ACHOWS_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFQ0046641.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(41442..42134)
                     /gene="rutB"
                     /locus_tag="ACHOWS_001019"
     CDS             complement(41442..42134)
                     /gene="rutB"
                     /locus_tag="ACHOWS_001019"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFQ0046642.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(42134..43225)
                     /gene="rutA"
                     /locus_tag="ACHOWS_001020"
     CDS             complement(42134..43225)
                     /gene="rutA"
                     /locus_tag="ACHOWS_001020"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFQ0046643.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDSLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAAHAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSDTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            43513..44151
                     /gene="rutR"
                     /locus_tag="ACHOWS_001021"
     CDS             43513..44151
                     /gene="rutR"
                     /locus_tag="ACHOWS_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFQ0046644.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(44191..48153)
                     /gene="putA"
                     /locus_tag="ACHOWS_001022"
     CDS             complement(44191..48153)
                     /gene="putA"
                     /locus_tag="ACHOWS_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFQ0046645.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPPVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGDGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            48208..48417
                     /locus_tag="ACHOWS_001023"
     CDS             48208..48417
                     /locus_tag="ACHOWS_001023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046646.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            48576..50084
                     /gene="putP"
                     /locus_tag="ACHOWS_001024"
     CDS             48576..50084
                     /gene="putP"
                     /locus_tag="ACHOWS_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFQ0046647.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
BASE COUNT        11936 a        12891 c        13537 g        11827 t
ORIGIN      
        1 agcggtaccg ggatgcacga tagagctgaa agcgcgccgc acaacggaga cggtgattgt
       61 caccacggtg gcgtatggtc agccggggga aaccggcagt tacagtatgg atgttgagcc
      121 ggggttgtac cgggtgacgc tgaacacgga agggtacgcg ccgtcatatg tgggtgacat
      181 tctggtgaag gcggattctg caccgggaac gctgaataaa tttctgatgg acctggagga
      241 cgcacagtat tacccgaaag cccttgcaga gctggaagcg gtggcagcgg aaatcctgaa
      301 acgtgcagaa gcgtcagcgg ccagtgcaga ggaagcgaag aaacgggcag agaatgcgcg
      361 gggaccgaag ggggataagg gagacaccgg gccgcagggt attcccgggc caaaaggcga
      421 taccggcgag cgggggccaa agggtgagcg tggtgagaca ggaccacagg ggcttcaggg
      481 tgtgaaaggt gaacggggag agaagggcga aaaaggcgag ccgggaggac cggatgcgac
      541 gacggcacag aagggaattg tgcagttaag cagcgcaacg gacagtgatg atgaaacgaa
      601 ggcagccacc ccgaaagcgg tgaaagcggc aatggacaaa gcggacggat gcctggagaa
      661 agcgaaaaac ggtgacgata tcccggataa ggtgaagttt ctgaacaccg tgggagcagc
      721 cagagtatac gggcgggaca ttcatacggg ggccggtgaa tggaccacga gcgagtttgt
      781 cgcctggctg aaagaaaaag gggcatttga ccagccttac tggatgatga aggcatcact
      841 gcctgcggta ctcaataaag tcatcacgga tgtcggaccg ggaaaactca atctgggtgg
      901 ctgtgtcatt gaggtgatgg ggacgtataa cgcagccata gtccgggtca ccattggcga
      961 atacggtgca acggggttta ttaatggcac ggtctgtacc tgtacggttt acggcgacac
     1021 acagtatttc cactggcggg tggattacag cacaaaaaac aaaccggata cggtcagcca
     1081 gcgggatgcc agcacgacgc agaaaggtgt ggtgcagtta agcagtgata ctggcagtaa
     1141 tgacgaaaca aaggcagcca cgccgaaggc cgtgaaggcg gcaatggatg tggcaaatga
     1201 agcgaaaaca aaggcagaag aggctgcagc aggaggtggt gttcccggtc cgaaaggaga
     1261 gaaaggcgac cccggagcac agggtccgaa aggtgaaacg ggagcaacag gccccgctgg
     1321 tccgcgggga gcgcagggac cgaaagggga gaaaggcgac cccggtccca gaggtgagcg
     1381 tggagaaacc gggccgatgg ggccgcccgg ggcatcagac gggaagagcc gggttgtggg
     1441 tatcaggctg ggtaatgaac agacttacag agcagaagag agctctggtt ttctgtcaat
     1501 aagcctgagt ataggaggtg tgattactgg tctgaaaagt agaggagcag atggtgtgat
     1561 atcggtttct tacaggccgc ttcaggtgac atttgatggt tctcagtggc gtacagtcag
     1621 cgtgggtgag tatgtgtcgt caggagcttc ggggtttgag tattttccat tataaagcgc
     1681 atcatctttc agacatgtaa ccgggaggac ttacagatgc acataaagaa tttcagacaa
     1741 tacacgccgg aaaatccgga tgtgccgggt gcgatgtacc tgaaatcaga ggatggtcag
     1801 gactggtatg agtgtcagtc gttattttca gcagagacgc tgaaggtggt ttataacagt
     1861 gccggggtca ttaccggcat cagcagggtg gcatcagttc tgtggccagt gggccagagt
     1921 gtggtggagg tggcggatac ggaagaaaac cgcaaagctg acatctcggg gcgctggggt
     1981 tttgacgggg agaaaatcac gggcctgctg accgcggaga aagcgcgagg gatgaagggc
     2041 gatgaaatta acgcctggcg taatgcgatg gaagcggcga actacatgtt tgagcacaat
     2101 gggcgtaaat gggactacgg gaagtcaacg cagacgcgtc ttgagccgtc ggtggcggca
     2161 gcgaaagcgg ggaaactgcc ggaggcgttt ttctggacgg atgcggaaaa caatgatgtg
     2221 gaggtgacag cagaagagct tatagcactg agtgaagcgg cagagcaggc gatgttcacc
     2281 aaagggatgg aaatccacgt ccggcagcgc accatgaaga aagagctgga aaagctgacc
     2341 agtgcggatg agatactggc atacagggtt ggctgggcac aggaataaca gacaaaaaga
     2401 tgggggacca tcacctcccc catgaactga gtcaattata cacttgtgaa gtatttacta
     2461 tgcgaataaa tactgtcacg gaaagtgcag gcctggatgt gatggaaatc atgtttccgg
     2521 cgcatcctgt tgatatggat aatttttcag atttccggaa gtccgtattt tgcccgtgcg
     2581 cggttacatg cttcattcgc gatgccattt tcgccggaca ttgcgaattc ccggcatgtg
     2641 gacggacggt tcttgtaaac agagcaatat gcgtttttac ccggagttcc cgccagagcg
     2701 acacatcggg gatttttctg actggtgccg cgcatacagc gatgaaaggg ggatatttgc
     2761 tcagtgagac tgaccggaac ctttccgcca gcatcgtcag cttctgccca gtaaaaagag
     2821 acgcggaaaa atgcacaaca ggcaccacac gtcatgcatg gattggggtt attcataatt
     2881 catcggtata gcgataaatc gtaaagagaa caatgtacct agtgtgtatt gagctaaata
     2941 taccaattag tataccaagt gtatactaag agcaaaaaaa gaaaggggct accatttggt
     3001 aaccccttga ttgtttggcg gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc
     3061 cggttttcaa gaccggtgcc ttcaaccgct cggccacact tccggaatga cgcgcactat
     3121 aaacatcccg atgcggcgtg taaaccccta atttgtttgt ttgcctgaaa aacagccaaa
     3181 agtgcattga tagcatgaaa taacagcaga ttgatcattt catcaccatg aattccttct
     3241 cttttactcg tttagcaacc ggctaaacat ccccactgcc cggcaaaaag aaaaataggt
     3301 ccatttttat cgctaaaaga taaatccaca cagtttgtat tgttttgtgc aaaagtttca
     3361 ctacgcttta ttaacattac tttctggcga cgtgcgccag tgcagaagga tgagctttcg
     3421 ttttcagcat ctcacgtgaa gcgatggttt gccttgctac agggacgtcg cttgccgacc
     3481 ataagcgccc ggtgtcctgc cggtgtcgca aggagaagag acgtgcgata tgaataacga
     3541 ggaaacattt taccaggcca tgcggcgtca gggcgttacc cggcgcagct ttctcaaata
     3601 ttgtagtctg gctgccacgt cgctgggatt aggcgcggga atggcaccaa agattgcctg
     3661 ggcgctggag aacaaaccgc gcattccggt ggtatggatc cacggtctgg aatgcacctg
     3721 ctgtaccgaa tcttttatcc gctccgctca cccactggcg aaggacgtca tcctttccct
     3781 gatttccctc gattatgacg atactttgat ggctgccgcc ggaacccagg cggaagaagt
     3841 ctttgaagac atcatcacgc aatacaatgg caaatatatc ctcgcagtag aaggtaatcc
     3901 gccgctgggc gagcagggga tgttctgtat cagcagcgga cgaccgttta ttgagaaact
     3961 caaacgtgcc gctgccggag ccagtgcgat tatcgcctgg ggaacctgcg cgtcctgggg
     4021 ctgcgtgcag gccgcgcgac ccaatccgac gcaggcaacg cctatcgaca aagtcatcac
     4081 cgacaaaccc attatcaaag tacctggctg cccgccgatc ccggatgtga tgagcgccat
     4141 cattacttac atggtgacct ttgatcgctt gccagatgtc gacaaaatgg gccgtccact
     4201 gatgttttat ggtcagcgta tccacgataa atgttatcgc cgcgctcact tcgacgccgg
     4261 agagttcgtc cagagttggg atgatgacgc tgcccgcaaa ggttactgcc tgtacaaaat
     4321 gggctgcaaa gggcctacca cctataacgc ctgttcctcc acacgctgga acgatggcgt
     4381 ttccttcccg atccagtcag gtcacggctg cctgggttgt gcggaaaatg gtttctggga
     4441 tcgcggttcg ttctacagcc gcgtggtcga tattccgcaa atgggtaccc attccaccgc
     4501 cgataccgtc ggtttaaccg cacttggcgt ggtggcagcg gctgttggtg tgcacgcagt
     4561 cgccagcgcc gttgaccagc gcagacgcca taaccagcaa cctacagaaa ccgaacatca
     4621 gccaggcaat gaggataaac aggcatgagc actcagtacg aaactcaggg atacaccatc
     4681 aataatgccg gacgccgcct ggtggtcgac ccgattacgc gcattgaagg ccacatgcgc
     4741 tgcgaagtga atattaacga tcagaatgtg atcaccaatg ccgtctcctg cggcaccatg
     4801 tttcgtgggc tggagatcat cctgcaaggg cgcgacccgc gcgatgcgtg ggcgttcgtt
     4861 gaacgtatct gcggcgtctg tactggcgta cacgctctgg cttcggttta cgccatcgaa
     4921 gatgccatcg gtattaaagt gccggacaac gccaatatca tccgcaacat tatgctggca
     4981 acgctctggt gccacgatca tctggtgcac ttctatcagc ttgccgggat ggactggatc
     5041 gatgtgttag atgcgctgaa agccgacccg cggaaaacct ccgaactggc gcaaagcctc
     5101 tcctcatggc cgaaatcatc ccctggctat ttcttcgacg tacaaaaccg cctgaagaaa
     5161 ttcgttgaag gcgggcagtt ggggatcttc cgcaatggct actgggggca cccgcagtac
     5221 aaactgccgc cagaagccaa cctgatgggc tttgcccact atcttgaagc tctcgatttc
     5281 caacgtgaaa ttgtcaaaat ccacgcggtc tttggcggta aaaacccgca tccaaactgg
     5341 attgtcggcg ggatgccttg tgccattaac attgacgaaa gcggcgcggt cggggcggtc
     5401 aatatggaac gcctgaacct ggtgcagtca atcattaccc gtacggcgga tttcattaac
     5461 aacgtgatga tccccgacgc cttagccatc ggtcagttca acaagccgtg gagcgaaatc
     5521 ggcactggcc tttcggataa atgcgttctc agttacggcg cgttcccgga catcgccaac
     5581 gactttggcg agaaaagtct actgatgcct ggcggcgcgg tgattaacgg cgacttcaac
     5641 aatgtgctgc cagtggattt ggttgatccg cagcaggtgc aggagtttgt cgatcacgcc
     5701 tggtatcgtt atcccaacga tcaggtcggg cgtcatccgt tcgatggcat taccgacccg
     5761 tggtacaacc ccggcgatgt caaaggcagc gataccaaca ttcagcagct gaatgaacag
     5821 gaacgctact cgtggatcaa agcgccacgc tggcgcggta atgcgatgga agtggggccg
     5881 ctggcacgca cgttaatcgc ttatcacaaa ggcgatgcgg cgaccgttga gtcggtcgat
     5941 cgcatgatgt cggcgttgaa cctgccgctt tccggcatcc agtcaacatt aggccgcatt
     6001 ttgtgtcgcg cgcacgaagc gcagtgggcc gcaggcaagt tgcaatattt cttcgacaag
     6061 ctgatgacca atctcaaaaa cggcaatctc gccaccgcct ccactgagaa gtgggaacca
     6121 gcaacctggc cgacagagtg ccgtggtgtc ggctttaccg aagcgccgcg cggagcgtta
     6181 ggccactggg ccgccattcg cgatggcaag attgatctct accagtgcgt ggtgccgacc
     6241 acctggaacg ccagcccgcg cgatcctaaa gggcaaattg gcgcttatga agcggcgctg
     6301 atgaacacca aaatggcgat ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc
     6361 tttgatccgt gcctcgcctg ttcaacacac gtgctgagcg acgacggtag cgagctgatc
     6421 tccgtgcagg tgcgttaaca gcgaaggaga atcatcatgc aacagaaaag cgacaacgtt
     6481 gtcagccact atgtctttga agcgccagtg cgtatctggc actggctgac ggtgttatgc
     6541 atggcggtgt tgatggtcac cggttacttt atcggcaagc cgctaccttc cgtcagcggc
     6601 gaggcgacgt atctgttcta tatgggctac atcaggttaa ttcacttcag cgccgggatg
     6661 gtttttaccg tggtcttgct gatgcggatc tactgggctt ttgtcggcaa tcgctactca
     6721 cgcgagctgt ttatcgtgcc agtatggcgt aaaagctggt ggcagggcgt gtggtatgaa
     6781 atccgctggt atctgtttct ggcaaaacgt ccgagtgccg atataggtca taaccccatc
     6841 gcccaggcgg cgatgttcgg ctatttcctg atgtcagttt ttatgatcat caccggcttt
     6901 gcgctgtaca gcgaacacag ccagtacgct atttttgcgc cgttccgtta tgtggtggaa
     6961 tttttctact ggacgggcgg caactcaatg gacgttcaca gctggcatcg gctggggatg
     7021 tggctgattg gcgcgtttgt gatcggtcat gtctacatgg cgctgcgtga agatatcatg
     7081 tccgacgaca cggtgatctc caccatggtc aacggctacc gtagccacaa atttggcaaa
     7141 ataagtaaca aggagcgttc atgagcgagc aacgcgtggt ggtaatgggg ctgggcaacc
     7201 tgctgtgggc cgatgaaggc ttcggcgtgc gggtggcgga acggctgtat gcccattacc
     7261 actggcccga gtatgtggag attgtcgatg gcggtactca gggactgaac ttgctggggt
     7321 atgtcgaaag cgccagtcac ttgttgattc tcgatgccat tgactacggg ctggaaccag
     7381 gaacgctgcg aacctatgcc ggagaacgca ttccggctta tctcagcgcg aagaaaatga
     7441 gcctgcacca gaacagcttc tccgaagtgc tggcgctggc ggatatacgt ggtcatttgc
     7501 cagcacatat tgccctcgtc ggtctgcaac ccgcaatgct cgacgactac ggcggtagcc
     7561 tgagcgaact ggcgcgggag caactgcccg ctgcggaaca atcggcgctg gcgcagctgg
     7621 ctgcgtgggg aattgtgccg caaccggcta aggaatcgcg ctgtctcaat tatgactgtc
     7681 tgtcgatgga aaattacgaa ggcgttcgct tgcgtcagta caggatgagg ctggaggaac
     7741 agggatgagc aacgacacac catttaatgc gttgtggcaa cgaatgctgg cgcgcggctg
     7801 gacgccggtc agtgaatgcc gtcttgacga ctggcttacg caagcgccag acggcgtggt
     7861 gttattaagt agtgacccga aacgcacgcc agaagtcagc gataacccgg taatgattgg
     7921 cgaattactg cgcgagtttc ccgactatac atggcaggta gcgattgctg atcttgagca
     7981 gagcgaagcc atcggtgatc gttttggcgt atttcgtttt cccgccacac tggtgtttac
     8041 cggtggtagc tatcgcggcg tgctgaatgg tattcacccg tgggcggaac tgataaacct
     8101 gatgcgtggg cttgtcgaac cacaacagga gcgtgcctca tgagcgaaac ttttttccat
     8161 ctgttggggc caggaacgca accgaacgat gacagtttca gcatgaatcc actgccgatc
     8221 acctgtcagg tgaatgatga accgagtatg gcggccctgg agcaatgtgc tcacagcccg
     8281 caggtgattg cgctgttaaa cgagttacaa caacaaataa gcgaacgcca gccaccgttg
     8341 ggcgaggtgc tggcagtcga tctgttaaat ctcaacgccg acgatcgtca ctttatcaat
     8401 acgcttctcg gggaagggga agtgtcagtg cgcatacagc aggctgacga cagtgaaagt
     8461 gaaatacagg aggcgatctt ctgcggatta tggcgggtgc gcagacgtcg cggcgacaag
     8521 ttgctggagg acaaactgga ggctggctgc gcgccgctgg cattgtggca ggcggcaacg
     8581 caaaacgtct tgccgacaga ttcgctgtta ccgccgccca ttgatggcct gatgaatggc
     8641 ctaccgttgg cgcatgagct actggcgcat gtacgtaacc ccgacgcgca gccgcacagc
     8701 attaatctga cgcaattacc catcagcgag gctgatcggc tttttctctc acgtctctgt
     8761 gggccgggaa atattcagat tcgcaccatt ggctatggcg agagctatat caacgccacg
     8821 gggttacgcc atgtctggca tttacgctgc acggacacct taaaaggccc gttactggaa
     8881 agttatgaaa tctgcccaat accggaagtg gtgctggcag cgccagaaga tttggtcgac
     8941 tctgcgcagc ggcttagcga ggtatgccag tggctggcgg aaggtgcacc gacgtaaaaa
     9001 gacggtaagt atcgctttca ggcttatgaa tatcgcaatc ggcgaatacc tctggtcgta
     9061 gagtttcagg ataaagaggg agatctacca ttaacgggtt atttttctct cttcgcctac
     9121 aggagtgcgc atgtgggatg tcattgattt atcgcgctgg cagtttgctc tgaccgcgct
     9181 gtatcacttt ttatttgtac cccttaccct ggggctgatt tttttgctgg ctatcatgga
     9241 aaccatttac gtggtcaccg gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa
     9301 gctcttcggt atcaactttg ctcttggcgt ggctaccggc ctgacgatgg agtttcagtt
     9361 tggtactaac tggtcattct attccaacta tgtgggcgat atttttggcg caccgctggc
     9421 gatggaagca ttaatggcct tcttcctcga atccaccttt gtcgggctgt tcttcttcgg
     9481 ctggcagcgg ctgaataaat accagcacct gctggtgacg tggctggtgg cgttcggttc
     9541 aaatctctct gcattgtgga tattgaatgc caacggttgg atgcaatacc cgaccggtgc
     9601 gcattttgat atcgacaccc tgcgtatgga gatgaccagt ttcagcgagt tggtctttaa
     9661 cccggtcagc caggtgaaat ttgtgcacac tgtaatggcg ggctacgtga ccggggccat
     9721 gtttattatg gcgatcagcg cctggtattt actgcgcgga cgggagcgcg atgttgcatt
     9781 acgctcgttt gccatcggtt ccgtcttcgg tactctggcg attatcggca cgctgcaact
     9841 cggagacagt tctgcgtatg aagtcgcgca agtacaaccg gtaaaactgg cggcgatgga
     9901 aggggagtgg caaacggaac ctgcacctgc accgttccat gtggttgcct ggccggaaca
     9961 ggatcaagag cgtaacgcct ttgctatcaa aattcccgcg ctgctaggga tcctcgccac
    10021 tcactcatta gataaacccg tgccgggtct gaagaatttg atggctgaaa cctacccacg
    10081 cttgcaacgc ggacgtatgg cctggctgtt aatgcaggaa atatcgcaag gcaatcgtga
    10141 gccgcatgtg ttgcaggcat ttcgggagct ggaaggcgac cttggttacg gcatgttgct
    10201 ctcccgctat gcgccggata tgaaccatgt cacagctgca cagtaccagg cggcgatgcg
    10261 tggcgcgata cctcaggttg cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg
    10321 ttctctgctg ctactggtga tgctgattgc gcttgtccag acgctgcgtg gcaaaattga
    10381 ccagcatcgc tgggtgctga aaatggcgct ctggagtttg ccgctgccgt ggattgcgat
    10441 tgaagccggg tggtttatga ccgagtttgg tcgtcagccg tgggcgatac aggacatctt
    10501 accgacatac tccgcgcact ccgccttaac cacaggacaa ctggctttct cactgatcat
    10561 gatcgtaggg ctttacaccc tgttcttaat cgccgaagtc tacctgatgc agaaatatgc
    10621 ccgtctgggg ccgagcgcga tgcagagtga acaaccgacg cagcaacagg ggtaaaggag
    10681 aaaatcatgt ttgattatga aacattgcgc ttcatctggt ggctgctgat tggcgtgatc
    10741 ctggtggtct ttatgatctc cgacggattt gacatgggga tcggctgtct gctgccgctg
    10801 gtggcgcgta atgatgatga acgtcggata gtgataaaca gcgttggcgc gcactgggaa
    10861 ggcaatcagg tctggttgat cctcgcaggt ggggcattat ttgccgcctg gcccagagtg
    10921 tatgcagcgg cgttttccgg cttttatgtg gcgatgattc tggtgctgtg ctcgctgttc
    10981 ttccgcccgc tggcctttga ttatcgcgga aaaatcgccg atgcacgctg gcgtaaaatg
    11041 tgggatgccg gactggtcat cggcagtctg gtgccgccgg tagtcttcgg tattgcattc
    11101 ggcaacttgc tgctcggcgt gccgtttgct ttcacaccgc aattacgcgt ggaatatctc
    11161 ggcagcttct ggcaactgct gacgccattc cctttattgt gtggattgct cagtcttggg
    11221 atggtgattt tgcaaggtgg cgtctggtta caactgaaaa ctgttggtgt gattcatctg
    11281 cgttcacagc tggcgaccaa acgcgccgca ctactggtga tgctgtgctt tttgctggcg
    11341 ggttactggc tgtgggttgg tattgatggc tttgtactgc tcgcccagga tgctaacggt
    11401 ccttccaatc cgttaatgaa actggtggca gtgctacctg gtgcctggat gaataatttc
    11461 gtcgaatcgc ccgttttgtg gatcttcccg ctgctggggt tcttctgccc attgctgacg
    11521 gtgatggcga tttatcgtgg tcgcccgggt tggggatttt tgatggcgtc attgatgcaa
    11581 tttggcgtga ttttcacggc aggcatcacg ctgttcccct ttgtcatgcc gtcaagcgtg
    11641 agtccgatct ccagcctgac gttgtgggac agcacttcca gtcagctgac gctgagcatt
    11701 atgttggtaa tcgtgctgat atttttgcct attgtgttgc tctacactct ctggagctac
    11761 tacaaaatgt ggggacgcat gacgacagaa actctccgcc gtaacgaaaa cgagctgtac
    11821 taaggagcag gaacaatgtg gtatttactt tggttcgtcg gcattttgtt gatgtgttcg
    11881 ctctccaccc ttgtgttggt atggctggac ccgcgtctga aaagttaacg aacgtaagcc
    11941 tgatgtggtg cattagcatc gcatcaggca atcaataatg tcagatatga aaagcggaaa
    12001 catatcgatg aaagcgatct taatcccatt tttatctctt ctgattccgt taaccccgca
    12061 atctgcattc gctcagagtg agccggagct gaagctggaa agtgtggtga ttgtcagtcg
    12121 tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg caggatgtca ccccagacgc
    12181 atggccaacc tggccggtaa aactgggttg gctgacaccg cgcggtggtg agctaatcgc
    12241 ttatcttgga cattaccaac gccagtgtct ggtggctgac ggattgctgg cgaaaaaggg
    12301 ctgcccgcag cctggtcagg tcgcgattat tgctgatgtc gacgagcgta cccgtaaaac
    12361 aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca ataaccgtac atacccaggc
    12421 agatacgtcc agtcccgatc cgttatttaa tcccctaaaa actggcgttt gccaactgga
    12481 taatgcgaac gtgactgacg cgatcctcag cagggcagga gggtcaattg ctgactttac
    12541 cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg cttaattttt cgcaatcaaa
    12601 cttgtgcctt aaccgtgaga aacaggacga aagctgttca ttaacgcagg cattaccatc
    12661 ggaactcaag gtgagcgccg acaatgtctc attaaccggt gcggtaagcc tcgcatcaat
    12721 gctgacggag atatttctcc tgcaacaggc acaaggaatg ccggagccgg ggtggggaag
    12781 gatcactgat tcacaccagt ggaacacctt gctaagtttg cataacgcgc aattttattt
    12841 actacaacgc acgccagagg ttgcccgcag tcgcgccacc ccgttattgg atttgatcat
    12901 ggcagcgttg acgccccatc caccgcaaaa acaggcgtat ggtgtgacat tacccacttc
    12961 agtgctgttt attgccggac acgatactaa tctggcaaat ctcggcggcg cactggagct
    13021 caactggacg cttccaggtc agccggataa cacaccgcca ggtggtgaac tggtgtttga
    13081 acgctggcgt cggctaagcg ataacagcca gtggattcag gtttcgctgg tcttccagac
    13141 tttacagcag atgcgtgata aaacgccgct gtcattaaat acgccgcccg gagaggtgaa
    13201 actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc atgtgttcgt tggcaggttt
    13261 tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt ttgtaatgca taaaaaagag
    13321 cattcagtta tctgaatgct ctgaggctga tgacaaacga agaactgtct aatgcgtaga
    13381 ccggaaaagg cgttcacgcc ccatccggcc accttacctt cagttttact ctttctcgga
    13441 gtaactataa ccgtaatagt tatagccgta actgtaagcg gtgctggcgc gtttaatcac
    13501 accattgagg attgcgcctt taatattaac gccagcctgt tccagacgct gcattgacaa
    13561 gctcacctct ttggcggtgt tcaagccaaa acgcgcaacc agcaggctgg tgccgacaga
    13621 gcgtcccacg accgcagcat cgctcaccgc cagcatcggc ggcgtatcga caatcaccag
    13681 atcgtaatgg tcgttcgccc attccagtaa ttgacgcatc cgatcgcgca tcagcagttc
    13741 agacgggtta ggtggcacct gaccgcgagt aatcacatca aagcctcctt tgccaaaatg
    13801 ctggatcact ttgttgagct catctttacc tgccagatat tccgacaagc catgttcatt
    13861 actcacggta aacaggttat gcgaataacc acggcgtaag tcggcatcaa taaataacac
    13921 tttttgatcg gactgggcga tcaccgctgc cagagttgaa ctgacgaacg ttttaccact
    13981 gtctggcgtc gcaccggtga tcatcagaat gttattctct gtctccatca tagcgaagtg
    14041 cagactggtt cgtagcgcac gtacggcctc cacagcagaa tccgccgggt tatccaccgc
    14101 caggaagggg atatttttag tacgatggcg ctgctgatta gaaaataaat ttttcttacg
    14161 cagacgggta cgtttatcca gccactcgga cattgggata gtggcataaa cgctgatgcc
    14221 gtgctcttcc agttgttccg gggcttctac accacgacgc aacatcgcac gcgccagcac
    14281 ggcacccacc gaaataaaca ggccaagaat aaaaccaagc accacattca acgctttttt
    14341 cggtttcact ggctgcggct gagtgactgc cgggtcgata atccgcacgt taccaatcgc
    14401 actggatttc gaaatactca actcctgctg gcggttaagt aattgcagat ataccgcacg
    14461 gcccgcttct acgtcacgac ttaaacgcaa cacttcctgt tgggtggaag gcattgccga
    14521 tacccgctta ttcaggcgtt tgcgttcttg ctccagtgtc tggcgttttt ccagcagcgc
    14581 acgataagtt gggtgatctt tcttatacag ctgggagatc tctgcctcgc ggaaagtcag
    14641 ctcattgagt tgattatcaa cgttcacaat ctgctcaaga acggctttgg cttccaggtt
    14701 aaggtcaacc gaatcgcgct gttggcgata aacgttgagt ttttcttccg cctggtccag
    14761 ctcgctgcgc acttctggca actggcgctg taagaattca aggctttgtg aatcctgcgc
    14821 cgcctggcga gcgatattct gttgcaaata gttgttagcg atgctgttca gaatacgagt
    14881 aatcaactgg ggatcatcac cggtcatggt aagttccagc atcccgcttt ctttactgcg
    14941 ttcgctaacg gtaaaggttt cctgcaacgc gttaatcgct tccagttcgg tacgctggct
    15001 caggacaaac tgcgttcctg gtttggcctt aatgtccgcg atagtcagcg caacgccgtc
    15061 tttttccaga cgctggccga ccataccatt gacggtgaac ccttcacctt ccagcgtata
    15121 gtggccgttt tccccaactg tgagtgtcag ttgctgatcc tgaccattca gttgtgggat
    15181 gtgcatccag ctgatcgcca gctcacctgg tttctctttg gttaatctcg cccagccgcg
    15241 acccacaatc ggaaaatact tctgctcaac catgtcgcgc agattcagtt cagcaatggt
    15301 tttaccgaga atcatgcgcg attgcagcag ttggatctcc ggtgctgact cgggcgatga
    15361 gttagggatc atatcgctca ggccgctgag aatggcgttg ccctgtttct gctcaacctg
    15421 gaccagagta tctgcctgat aaattggcgt gcttaacagc gagtaagcga cagcgatcag
    15481 cgtgaacaac gcggtcacgc tgacaataaa cttacggtga tcccataact cgccgaccag
    15541 acgaagcaga tcgatctcat tttcctgagt gctgcctggt ggcgtattca tatttttagt
    15601 tgtcatcgta ttttataaac tcaaattacc ggctgaggcg cttcgcccat tcctgactgg
    15661 cgcgctccaa cataccgtag acatgctcaa atgcgtcctg acttttacga tagggatccg
    15721 ggatctcttt ctgttccagc cattgcccaa acagcattgt cttcccgcgg acctcgggag
    15781 cgattgccgt aacctgagca atatgttccg actccattgc cagaatcaga tcgtaatttc
    15841 gtgccatctc agcagtgagc ttacgtccgg catgtccttc aagagatacg ccatgattag
    15901 cggcaacatc tgccgctgtc gcatctgcag ggtgctttac cagaccatga acacctgccg
    15961 atttgacttt tacgcctggc agacgtttac gcagcaagcg ctcgccaatg ggcgaacggc
    16021 aaatgttgcc ggtacaaacc accaggattg agttaaattt tagttgggcc atgtttgaat
    16081 ccaccgtgaa gtctctgtca ggtcatgcac cccggaaatc gtcggtacca gtagagaaat
    16141 cacacggttc caacgagcca gcggcgcagt agtgacataa acaatgtcgt aaggttcaag
    16201 ctggaattcc gttccgagta tcattgccga tgcgtccttc gcattcagct gataaatatt
    16261 ggcgattttg ccattctgct tgttttgcgt tgcacgaata acaaaaatcc ccgtcgcatc
    16321 agcgacatcc tgattcatac cctcagcgtt acccagcgct tccgccaggg tcataccgct
    16381 gcggtccatt ttcagcgtgc tctgtttacc cacttcaccc atcacgaaga ctttcagatc
    16441 atcgttgcgc ggaataaata aaatatcgcc cgggtgcagc agtttgttct ggcgcaagtc
    16501 gccacgctgc atcagcgcat agaggttcac tttggtcttt acgccattct gggtgagcac
    16561 cacgttgcgc cagtctgcat ctgcggtcag cccccccgcg gcgttaatcg catccatgat
    16621 ggtcagagga atattggtaa tcggctgctg acccgatttg ctgacttcac cggtgacgta
    16681 cgctttttgc gagcggaacg ccgcgacgct gacatcgacc tgcgggcttt caatgactga
    16741 atccagacgc gctgtaatct cgttgcgtac ctgggtaagc gttttcccgg ccactttcag
    16801 gcgaccaata taggggtaga aaattgcacc atcagcgtta acccagttgc cggtatcact
    16861 ggcgctacgg tattgccctg cgggtgtggt tagttccggg tgatcccaga ccgtaaccat
    16921 caacacatca ccaatgccga tacgatattc atagttcgcg atttgctgct ccagctccgg
    16981 gttagcctgc gacataatcg gtttggcgcg cagctgatca atcaatcctg gcgtgacggg
    17041 atagatattg accattttgt ccaggtcata ctgattgtca ggtagttcaa tgacatcctt
    17101 gttactggtc gataaattct gaccaggaac aagggtacat gctgtcaaag acagaacagc
    17161 caatgtcagt accgaaaact taaaaatgtt ctttttcatt ggtttatatt tatcagtcat
    17221 tgtaatgtta tgaattacgt caggttattg tccaaccggt acttctcgct ctgccgtcat
    17281 tggatagagc tggtatttgc gtgacagcat ttgtccacca tcacgcgtca atggtgtcca
    17341 cgaaaccacc gcgcggttgc ggttaggtcc aatggtcatc aagtcgaacg gaatcgagat
    17401 ataaaaacct ttactaaagc cgccttcgcc gtagtcatct tttgatacgt tactgattgc
    17461 cgcccatacc cctactgcca cgccgctgtc aaagcgtttg gcgacgtcga tcgttgcacc
    17521 tttatctttt gccagatatt gcccaacgct aagtttcatc agtacgccat tgagcgtcgg
    17581 cgggttccag taagccgtca cgaaacctgt tggcgtagaa taatcggtga agcgcatcat
    17641 gttgtcccag tcacgctgct tcacgtagtt aacgtccaca cccagcgccc agctggcatc
    17701 tagcgggcga taaagcagct cggaaccgac accggcatac atcgtttcca gataaccgcc
    17761 atacacctgg ccatagaaac cgttgcctaa gtcggcaaag tagttcgctt gcaagttgtt
    17821 gagataaacg tcattgcgaa cgtaatcacg gatatgcgtg cgcacgcggg gcagggtaga
    17881 gtccgcgggt aatagcgaag acttaaactt gtcgtagtta ttgtaaatat tggtgaaaat
    17941 accgccatca agcagcaggt ggtcggtaaa ccagtagcgg gcgctggaca tcagccccag
    18001 ctggaacata tagaaatctt ccgggccgcc cagcgactgt gaaagtgttg ggttgaaaga
    18061 gtagctaaag cgatcttcac gaatacggta gccccgacca aaggcagaaa gatcttcagc
    18121 ttcaacacgt tgttgttgca gtggctctga ttgaccaggc gctgtaccgg ccagttgttt
    18181 gcgcaggctg gctacgtcgg tttcggtagt caccatcgcc atatgctcgc gcttttgcgt
    18241 cacgctaatc ttctcaacgc cttgcggcag gttattcacc agaatccggt tggcacgatc
    18301 gaccgcttcg cgagagtcac ggtatttgta ttgctgacca gacatataca gcgtcttatc
    18361 gcgtagctga atttctggcg cgtcaaagcc cgcgttatac ttcagggcgg taagttggtt
    18421 tgctaccgtg gtgtactgca atccttcaga ttccggcgca ggttgatatg ccggttttgg
    18481 cgtatcgcgc agggcagggc gcagatcgtt gaaattggtc cgtaacgtga agccaaacat
    18541 caacgtgtta ccgcgttcat aactcaggtt gagatctgcc cagctggcag cgcgatagac
    18601 tgcgccgacg ttgaaatggc ttgcctgagg cagtttgccc gcaaaatcat tctggtaatt
    18661 gttgccatcg tattcgagtt tcagacgcag aggattccac ggcgtttgat actcaatacc
    18721 gccaaaaata gaggctggac cacgaaagat atcgctaaag ctgatatctc ccgcatcgtg
    18781 agactctgcg cgatggcaat atttatcgct tacccgacaa aacgggttgg taatattgcc
    18841 cgcattaccg gcgtagcccc aggccatccc gagggtgaaa tcgaacgggc ccgcttgctt
    18901 gctggccacc aaatattcac cgtcaaacag acccgtacca gcaatatcac gtttaccaaa
    18961 cgccacttgc ggtagccagt aaccttcttc ccacaggcga agtttaaaat cgaaagattt
    19021 gtctttgtat gactgatcgc cgctgaaatc ttcccactgg ctatatttgc gtgtgcgcac
    19081 atccgtataa cgaatggtgc cttccagcca tgggaacagc gccacggagg tggagtagaa
    19141 acggtattga tcgttatccc ggtagtttac gctgaattca ccttccggtg cgatgcgcgc
    19201 atttggcatc tgcaacaatc ctgtgccgcc aaagtctgat tgcgacgaac ccagcggatc
    19261 cggataggtt aatacttcag catgacaggc actggagacg gcaatggcca ggcagcttaa
    19321 aagataagag ttcttcttca tcagtcagga acacgttgcg ttaaaacaga cacaatctgg
    19381 tcatttaaat cagcatattt ttcgggcagt acatgcgcag aaaagcctaa ccaaagctgg
    19441 ctgccaggcg gtggttccac atgtctttta ttccacaggg caacaggggc gacgacagcc
    19501 tcaccctcag gcgtaatcac cataacgtta ttcttgtccg cgcctgcaag acgggtgtga
    19561 tcttgcagat aatcggtaac gctacggcct gccagccagg gcaattgccc agccccagaa
    19621 accgcaccta gcagagtaat agtcaccgga cgctgtacgg tatagagcgt atagtccccg
    19681 accagcgggg gattgctgtt ctcatccact cgtacaaaat cagggtccag tttgaccggt
    19741 aagcgaccag tgatattgag attgagtaac tgctggcgca cggatttaat cgttgccacc
    19801 acatcgtcgt cagcttctgc ttcccaggaa gcaagctgtg ccatgacatg ttggtagtct
    19861 ttaagcgctt tagctttcgc cgcagagtcg gtcagcaagg caccaggcca ccagaggcga
    19921 tcgcgcagtt gcggttgtgt caccaactgg acaacatttt ccacggggcc aacggaaagt
    19981 gtctgttgtt cgccaggcag ataaatagtc accgttcctt gcgcaaaggc atggggtgtc
    20041 attacgtaaa gtacgctggc aatgaaatac gactgtaatt tattcatggt gcgggtttaa
    20101 gaaacgtcat ttctacggga ataacccctg cgcctaacat ttgccgactt tgacgaacct
    20161 ggccagtagc cgaatcgacc cagaatgtgt tttgccactg tttttctggc agcgtggaag
    20221 tcacctcttc ctgccagaca ttacacagca catcgcgacc cgcaatattc agcgtctcta
    20281 atccagcaaa ggaaaaagtg gaactcaggg tcgcagagcg aaaatggttg tcttcactcc
    20341 agagaatatc gcgcgtccag cggctgccgt cttttattgc cagcgcgtta cgcagagggt
    20401 cctgcccgga atttgtcact tccagcagat tattattaag tttgacggtt tttaaaagac
    20461 gtccattgtg ggtaaccagc atggcgttat cctgggataa ccatttgctt tgttcttgtt
    20521 ctatatatcc cagtaccaca aagattcgtt gcccttcatt aaggcgtaaa tacatcgtgg
    20581 aataaggcaa cgcctggatc tgctgatccg ctacggtgat atcctgatta tcgaaaaggc
    20641 tggcgcgaaa tgtatcgacc atactttgct ggctgtgcgt acatcccgca agaaatagtg
    20701 cgaaaatcga taaaataaga gggcgcacct ttaatccttg aaaggttaat taaaaaaatg
    20761 caaatctcta aagcccggta cgaatatcgc aaccgggtat catttaactt taattttgac
    20821 gcgttaaagc agtattagcg cgtagtggac gtggtggttg aggtgttatg agaaccgcca
    20881 tcgccactgt catttgctgc aactactgcc aacgtcgcca caccaacagc cgttgccaca
    20941 ccgacaaccg tcgccgtgcc gacagcgcca gcagccacgc caccagtggt agcagcagtc
    21001 gtggttgcac ctgtagtggt cgttgtggtg ccagtagtac ccgtcgttgc ctcagtcccg
    21061 tttgctgctt caggggcagc aaatgcagct ggcgtcgtca acatgaaggc cataaggatt
    21121 gcagaaagct tgtgtttcat aacttttcct ttattcatcg catggacaat acgtacgctt
    21181 gagatcagga ttatctactt tgagtataac atccttgtga aatctccctt tcctcatacc
    21241 gccagcacta tatactgcgg gtgggaaagg cgcgcagagg gaaatataag attgttaact
    21301 attcccctct ggaaatacga cttgtgagca taagataaat cttagcaaaa tacaaattaa
    21361 agataagaaa attgttaaga aagctattaa ctattgttgg taatatagtt tcaagtggta
    21421 ctataactgg ctgttaagtg gcatttataa ctattaggtg cagagatatt cgcttaaatg
    21481 gatttgttgc tcggtgaaaa agtaacttgc gttataattt atggtgttga ctattgagct
    21541 attccaataa aaccagccaa ctttaaacaa gcaactcata ttaagagaga taaacatttg
    21601 ccgctgttgg tcctcgcagg ccatttacgc ggcaaaattc cacgcgtaat cctggtataa
    21661 gcacttctgc gtcgcgggga gtgaatgcgg aaatatggac ctggacttct ttacgaccgt
    21721 cggaggggat aatgaatcct ttgccgcttt tgcgatcaaa ggttttgaca attcctgtca
    21781 ttttacggga caaacaaatt ccttactgaa aatactgcgc tgcactatac ggggttaata
    21841 aaataaagcc agcgatattt aagaccgccg gacggctaaa ataaaatttg cttaatctca
    21901 attatcatgc gttaatagct gcgtcggttt gaaagacaga cagcatacaa agtagtttac
    21961 taaagcagtt ctcattatca ggcattatcc ccttcttttg agtctctctc ctgaacacta
    22021 agtagtttct gtactaaagc cctgtttgcc gaaaggccca aaatgaagga agtaaaatat
    22081 gtctaataaa atgactggtt tagtaaaatg gtttaacgca gataaaggtt ttggctttat
    22141 cactcctgat gatggcagca aagacgtttt cgtccatttc accgccatcc agagcaatga
    22201 attccgcacg ctgaacgaaa atcagaaagt tgaattttct attgagcagg ggcaacgtgg
    22261 tcccgcggca gcgaacgttg ttacgctcta aggttgccgt tattactcca catctccatt
    22321 tccgctgtcc atgttgtcat ggttcacagt accgcacatc ggcattcgat gtgacggagc
    22381 gaaatccttt aggggcaaag tgtatttttt gtaaatcgac gatgatcacc tttgataacg
    22441 tcgcgctgca aatacgcact gaccatgcgc cgctggattt cacaaaataa tatcaggctc
    22501 cctcgtggag ccttttttat atctgcctta tttttcttca acgctgtatg tatagtaagc
    22561 gataacctgt tgattattga atctttcggg gagatggctt ataacatttc ttacctgacc
    22621 agggtaccgg gaaccaacac cttactggcg tgttgctgtc tttcaagacc agaagaggtt
    22681 aacagtgaat attgaagagt taaaaaaaca agccgaaacg gaaatcgctg actttatcgc
    22741 gcaaaaaatc gccgagctga acaagaatac agggaaagaa gtctctgaaa ttcgcttcac
    22801 cgcacgagaa aaaatgaccg ggcttgaaag ttatgatgtc aaaatcaaaa taatgtgatt
    22861 ttgtgaacat cacccgtgcg aggtgatgtt ccgcttgttg ctaatttagt ggccaatcat
    22921 tggcgcttgt ggaattaagc gtcggtacaa ttcctccggc accgggcttt gccatactcc
    22981 cgcatacatt gcgtaaccaa tcaccgcaaa cataatcccc agaatcagta gcgtcattaa
    23041 ccagccagac aacgcaaagg cttttttatt tgccgcaggt ttttgcagtg aaaaggtcaa
    23101 tgttgaggct accggacatg actctacgca agtcatacag ccggtacatt ccactgttcg
    23161 tacctgaatt aatttatcga ccgggatccg tgatgggcaa ttttttgcgc atttgccaca
    23221 gtcgatacaa ctttcggcat tgcgacgaat cttaaacggc gataatagcg aaaccacgcc
    23281 catcagcgcg ccatatgggc aaagataacg acaccaggca tggcgaataa acaggctggc
    23341 aatcagcaaa acgatcatgc tgattaatgt cgcggtcccc atatgacgaa agaaattgag
    23401 cattttaacg tccatcacca cgctgtaggg cgacaacata aaatagtgaa tcgcctgagc
    23461 gggcatcaat aacgcaatat agagaaaaaa actcaacagc aaatacttca cgccgcgcag
    23521 aggagtatcc agccagcggg gaaggacaaa ttgccgacca aacagtttgt taccgagatc
    23581 gccgattaat tcagaaagcg taccaaccgg gcataaccat gaacaaaagg ccttttttaa
    23641 taatagactg atgacgataa aagcgaccaa taacagcatc gcggcggcgt ggacggacgg
    23701 taactggcct gttgcaaggc tatatttcag attcatcaga ccggcaatcg gtagccagcc
    23761 ttcgatacct cccggtctgg tgacaaatgt cgtactacct gccgtttcgt aatagcgcac
    23821 ccaataccag aacgtgatgg caatataaat attcattgcc aacagtaata attgcgtcgc
    23881 tttacgccag gtcgtggcat tgcgccagtc attccacggt aatttgccgc ccgtcgtgcc
    23941 tggccgccgc tgccagcggg ttcttctttt ctctgtcatg attttgccag tccgttaagt
    24001 tgcataccaa atgccactat tctagttgtt cttaactggc tgatattgat tcaaatcgcg
    24061 ttcaggtctt tcttatgcaa ccatgcttcc agagcggcaa cactgcgtgt aatttcttcg
    24121 tgtggaaggg gggcagataa tggctgctgc tccagttgtg cgcatagctg gctggcgata
    24181 cgcattccca ggctcgagca actgcttttt agctgatgtg cggcacgctt tattttctcg
    24241 ctatcctgat tggcgcgggc aatgtcgatg tcatcgagaa gcggcagggc atgttgtgta
    24301 aataatacca gccattcgtg gatcttctcc gtccccatta actgagcatc ttcattgagt
    24361 tgcgatacat ccagcggttg atcgttattg acttgcagtt ggagatagtg cgccagtaac
    24421 tgaccgagca cttcacgcgg caccggttta gggataatcc cgcgaaatag cgaactggta
    24481 cgctggcgca acgtttcgtc gatgacatgg gcgctaaagc caatcaaaac cagcgacgga
    24541 tattgctgtg ccagttgccg ggcaagcgta atgccgtcga tatccggcag atcaaaatcc
    24601 accagtgcga cagcaaatgg ttcactattt tgcagtgtct ctaaagcctg cgcggcattt
    24661 cctacggcaa cgacctgcgc cccactgctg ttcaacatct caacggtaat ccgctgggtt
    24721 agcgggttat cttcaattaa cagcaaacgt aaaccgtcca gacgcaccgc ctgattgact
    24781 gtttttggca cgggtgcagt ggcaatacgt aacagcaagc gtaaacaaaa acagcttcca
    24841 acctccggcg tgctggtggc gctcagttcg ccacccatcg cctgggccag acggctactg
    24901 atagtcagcc ccagcccggt gccgccgcgt ttgccgctaa cctgaacaaa tggctggaag
    24961 atttcagcca gtttcgcggg atcaataccg cagccgctgt cttccacttc gaccagccat
    25021 tgttcgccat cagtgcgact acgcaggata atgtgccctt cgtcggtgaa acgcagggcg
    25081 ttgctcaaca ggttggtaat gacctgacga atacgctgcg gatcgcccat taacgcagtc
    25141 ggtacatcgt cggcaattgc cgttgccagg cgaatcgggc gacctttcac ccgtccgctc
    25201 attaattgca gggtactttc cagcaacggg cgcggttcaa agggttcatc gctgaccgaa
    25261 acattcttgc cgcctgcttc gatagccgaa taatcgagaa tatcgttgag gatggtcagc
    25321 aacgattcgc cagagtcggt aattgcccgc aaatcatcac gctgagcgtt aagtgcgggg
    25381 ttatctgcca gtagctgtgc ggtaccgaga ataccgtaca gcggtgtgcg gatctcatgg
    25441 ctcatcgccg ccagaaacgc tgattttgcc tggttggctt tttctgcttc cgcccgtgcc
    25501 tgtcggtgtt ctatcaccag ttcctgcaat tcagctgtac gcgctttgac ctgcgccgcc
    25561 aactgttcac ggtggcgatt cagtgcatga acactgctgc gaaacgcatc catcagccgc
    25621 ccgatggtgt ccagttcccg cacgccagcg gtttccggga aaggggagtc gatatcacca
    25681 tccagcaagc gttgtagcgc ctgcgtttgt tctgcaagag ggcgcgtgac tgagcgataa
    25741 accacgcgcc agaggatcag aatcagtgcg caaagagaaa ctattcccag caataacagg
    25801 ctgtattgcc cgcgtgcact ggctttttcc agatgtgcca gcccgtgctg attacgcagc
    25861 tcaatggtgt cgaccagctg actgacttcg ctactaaact gcgcgaattg ggcgatgtta
    25921 ttttgggcga gagtttgcag gtgattgctg atttcactgt cctgctgata cagcgccagc
    25981 aaatcgctat attggctaac ggtagttaac gttgttgcta cctgagtacg aacacccgga
    26041 tcttcaatgc gtatttgccg acgttgcaga attttcaccg cattattgag ctgcttttcc
    26101 agcgttggcg catttttctg gatctgttcc agccccagat tcatcaccat ttgctgcacc
    26161 cgcagggcgc taaggcgcag ttcattcatc tggttaacat actcaagatc gatatcaatc
    26221 agccgatcga gtgcactttc tgcggcctga cgctgatgtt gttcgatcaa atcgtaaatc
    26281 ccggcctggg tcgctccggc ggaggttgcc gcattattcg cctgaccttg cgccaggcgt
    26341 gcgatttcat cggcggcggc gactatctgc tgactgagtt gctgttgttg ctggcgaagt
    26401 tgcaaacgct gccccaccag ttccccttgc tgacgtaacg aacgggagat ctcctgctcc
    26461 tgttgttcaa tagcggtggt gtcaaaatct tgttcccgta acgcttgcag caacgcatta
    26521 atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg
    26581 gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc
    26641 gccatattca tggtaggaat caatgcctga gtgttgtctt tttccacctg gctgataaag
    26701 cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag
    26761 cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcactttg ggtgaaaaag
    26821 gttgagtcgc aaagcggaat gcatctagca taaagcctta ttattgatga ggctatcatg
    26881 cgcgtactgc tattgttact actttccctt ttcatgttat cggcattttc ggctgataac
    26941 ctgttgcgct ggcatgatgc acagcattac acggtgcaag cctctacgcc gctaaaagcc
    27001 aaacgcgcat ggaaactgtg cgcgctttat cccagtttga aagattcata ctggttatcg
    27061 ttgaactatg gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag
    27121 gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg
    27181 ggcgcagagg ccattttgct cggtagtagc acgacgtcat ttcccgatct gcaaaagcag
    27241 gtagcaaatc tgccggtgat cgaactggta aatgctattg atgctcccca ggtgaaaagc
    27301 cgcgttggtg tgccctggtt tcagatgggc tatcaacctg ggcgatatct ggtgcaatgg
    27361 gcgcacggta acccactgaa cgtgctgttg atgcccggac ccgataacgc cgggggcagt
    27421 aaagagatgg ccgagggttt tcgcgcagct attgctggaa gcccggtacg tattgttgat
    27481 attgcgcttg gcgataacga tattgaaatc cagcgtaacc tgttgcagga gatgctggag
    27541 cgccatccag aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt
    27601 gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg
    27661 tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcagat ggtctggcag
    27721 ggggaactgg cggttgagca ggccatcagg caattacagg agcaatcggt gtctgataat
    27781 gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgcgaaca tatccgccgc
    27841 tcgctgtcac cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag
    27901 aaataacctt cgccatgttg cgtcaccagt aaatccgcgc tgagtttatg gcgtaaacga
    27961 cgaattaaca catcgacggt gcgcaagtca gggttttcca cccgacgcgc agagagcata
    28021 cgcagcagac gttcacggct aagaatttcg cccggattcg tcacaaatgc caccaacatt
    28081 tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac
    28141 acattcaggc aataaccggc aaagcgatag cagttgtcct gtgtgtgcgg ttgagcttgt
    28201 cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc
    28261 agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga
    28321 tcgctgcgtc ccgtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt
    28381 aacatcaggc cattttcatc gggcaaatta atatccagca gaattaaatc caccggctga
    28441 ttctgcataa tttcccgcag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc
    28501 tgagtgaagt aggattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg
    28561 tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatt ctgccgtaag
    28621 ccgttcatcc tgaccagtgc cgctgttcat atttactcat taagatcgct tcactaaacc
    28681 ataattttac aggggctatt atgcggaaac tctggaacgc gctacgccga cccagtgctc
    28741 gttggtcggt actggcgctg gtcgctattg ggattgtgat tggcattgcg ctgattgtat
    28801 tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta
    28861 tgcaaccggt gtatgaagaa tataaacagt cggtgcactt ccagaacgcc tccggcgtgc
    28921 gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac
    28981 tggaagcgag caacgacatt taccagacct ttattgccca ctccattgat acacctgaaa
    29041 aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca
    29101 actcggcaac ctgtcgctcc tgccataact acgatgcgat ggatcatgcc aagcagcatc
    29161 ctgaagcggc gcgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc
    29221 ataaaggtat tgcccaccag ttaccggata tgagtagtgg cttccgtaag cagttcgatg
    29281 agctgcgcgc cagtgctaat gatagtggtg acacgctgta ctccattgat attaagccga
    29341 tttatgcggc gaaaggcgat aaagaagcgt ctggttctct gctgcctgct tcggaagtga
    29401 aagtccttaa acgtgacggc gactggctgc aaattgaaat cactggttgg acggaaagtg
    29461 caggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc
    29521 gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc gacaccaata
    29581 ccgagtggag caagttgcag gctactgcgt ggatgaagaa aggcgacatg gtgaacgata
    29641 tcaaaccgat ctgggcttat gcggattcgc tgtacaacgg cacctgtaac cagtgccacg
    29701 gcgcaccgga aatctctcac tttgacgcta acggttggat cggcacgctc aacggcatga
    29761 ttggctttac cagcctcgat aaacgtgaag aacgcacctt gttgaaatat ctgcaaatga
    29821 atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa
    29881 taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt
    29941 cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc
    30001 gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg
    30061 cgcgacggtg aaggagggtc gcttcgtggc agcaaaaccg ttcgaactgg ataaatatcc
    30121 gtcgaaaatg atcgccggat tgccggatca tgtacacaac gcggcgcgta ttcgttatcc
    30181 gatggtacgc gtggactggc tacgtaagcg ccatctgagc gacacctccc agcgcggtga
    30241 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
    30301 acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
    30361 ggggatgttc cataacgctt cggggatgct ggcgaaagcg attgccttgc atggtaatag
    30421 cgtgggtaca ggcggagatt actctaccgg ggctgcgcag gtgatcctgc cgcgcgtagt
    30481 cggttcgatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa
    30541 aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc
    30601 ggatcacgat gtttatgaat attacgagca gctaaaagcg aaagtcgccg ccggtgaaat
    30661 tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
    30721 tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg ctctggcgca
    30781 tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtaggttt
    30841 tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg
    30901 ggctgaaaaa ctgacaggta ttaatgccga aaccattcgt gggctggcgc ggcagatggc
    30961 ggcgaacaga acgcagatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca
    31021 gtgggcgtgg atgattgtcg ttctggcggc gatgttgggg caaattggcc tgccaggtgg
    31081 aggttttggt tttggctggc actataacgg cgcaggcacg ccggggcgta aaggcgttat
    31141 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gcgactacaa
    31201 aggctacagc agcaccattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
    31261 agtgatcaac tggaacggta aatcggtaaa actaccgccg ctgaaaatgt gtatttttgc
    31321 cggaactaac ccgttccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
    31381 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
    31441 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
    31501 caatcgtggc attatcgcca tgaaacaggt cgtgccgccg cagttcgagg cgcgcaacga
    31561 cttcgatatt ttccgcgagc tgtgccgtcg ctttaatcgc gaagaagcct ttaccgaagg
    31621 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac agggcaaagg
    31681 acgcggcgtt catctgccag cgtttgatga cttctggaat aataaagagt acgtcgagtt
    31741 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
    31801 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgctg atatgaacta
    31861 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
    31921 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
    31981 gcagttatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggca aagagccagt
    32041 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
    32101 gtttaacgct cgcggtcagg tgttggcagg ggcagtggtt tctgaccgct atgcacccgg
    32161 cgtggcgcga attcatgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    32221 gctgtgcaaa tacggtaacc ccaacgtgtt gaccatcgac atcggtactt cgcagttggc
    32281 gcaggcgacc agtgcgcata ccacgctggt ggaaattgag aagtacaacg gaacagtgga
    32341 gcaggtgacg gcgtttaacg gccctgttga gatggtggcg cagtgcgaat atgttcccgc
    32401 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    32461 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    32521 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    32581 gagctggaaa accgtattgc cactcagaca gtacgtgacg atgctcgtct ggaactggcc
    32641 gctgactttt gcggcctgtt tttgatgacc gacaaacaag ctgcgctgcc gtatgcatcg
    32701 gcctacaaac aggacgagca agagattaaa cgcttgttag ttgaggcagg gatggaaacc
    32761 agcggcaatt tcaacgaacc ggcagaccat ctggcgatct atctcgagtt gttgagtcat
    32821 ctgcattttt cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacaa tctgcggcaa
    32881 aaaacactga cggcgctgcg acaatggtta ccagagtttg ctgcgcgttg ccatcagtac
    32941 gacagtttcg gtttttacgc ggcactaagc cagttattgc tggtgttagt ggaatgcgac
    33001 caccaaaaca gataacgtcg tttgttggcc tgataagacg cgtttagcgt cgcatcaggc
    33061 attagtgcgc agttgccgga tgcggcgaga acgccttatc cggcctacgg gaacagtgct
    33121 ttttgtgggc ctgataagat gcgttaagcg tcgcatcagg cattatggcg cagttgccgg
    33181 atgcggcgag aacgccttat ccggcctacg ggaacagtgc tttttgtggg cctgataaga
    33241 cgcgttaagc gtcgcatcag gcaacaaact cacgggtgag ccacaaaccg ggaaagccgc
    33301 tggcgcagca ggcggttttc ctgcttcagg tgcgcaatat catccattaa cgtcagcgcc
    33361 accgcgatcc ccggccagtc cagagccagt tcatgacgca ggcgtaccgc gcgttgcacc
    33421 acaatggcgg catggtcgtc aaatacccag gttgtctcct ggatctcccg cggttcaacc
    33481 acccccaaac cgacaatttc attcaactcc tcttcagaga tgccggtatg caggcaaaat
    33541 tcggtaatag taaaagtcac cgtaacatta gccattatgc tttcccccaa tctttacgtg
    33601 gatcaaaaga cgactgggcg tctgccagtt gctgccacag cgcggcagtg ttttcatccg
    33661 gtttcggcgg catcacgatt ttcagtaccg catacagatc gccggtctgt tttttgctca
    33721 ccagaccttt gcctttaacg cgcaatcgtt gcccggcctg gctgccaggc gggatagtca
    33781 gcaaaatgct ttctttcagt gttggaacgg tgactttagt acccagcgcc gcttcccacg
    33841 ggctaaccgg caccactatt tccagatcct ggccgacaat atcaaacagc ggatgtggcg
    33901 caatgtgaat caccagccac aggtcgccat ttggaccgcc gttttcgccc ggcgtcccct
    33961 ggcctttcag gcggatgcgt tgaccattgc cgacgcccgc cgggatcttc acattcagcg
    34021 ttttcgggat ttcctgttcg atcatgccaa aggcgttata aaccggcagg ttatagctga
    34081 tggtacgctt atgctcagta agcgtttctt cgaggaaaac cgccacttcg atttcaatat
    34141 cgtggccgcg tgtggcgggg cgttgacggc tctggcgggc atgctgaccg aaaattgacg
    34201 agaagatatc gtcaaaatct tcggcgttaa aactctgacc gtcgccatga tggaactgac
    34261 ggttaaattg cggatcgttg cgatgttgcc acatctgatc atactcagcg cgacgttgtt
    34321 catcacttaa cacttcccag gcttcggcga cctctttgaa gcgggcttcg gcatccggtt
    34381 ctttgctgac atcaggatgg tatttgcggg ccagtcgacg ataggcggtc ttgattgtct
    34441 tgagatcgtc cgtcggtttc acgcccatga tggcgtaata atcctttaat tccatagcgt
    34501 tatctcgcgt aaatcaacac aaattgaagg aacccctgta aggtaactcc tataagtgta
    34561 gggtaatcct caaaatttca tatgccaaca cagaatatgt tattgaaatc atcgcggagg
    34621 ggaggtcgcc tcaaggtggg aggaacatat tttatttacc tccctcctga acttttggct
    34681 catgcttaca agttaaacaa taaggaaagg aatgcacaac tcgactattt aaatcgttat
    34741 gcaatttaat gataaagaca tgtgctaaaa accatttcta ttagtctcac attctttttt
    34801 tattggtaat ggtaacgccg ctgattttct ttatagctac tgatgtaatc attagcggcg
    34861 taaacgaggc cggggctaat caggcatagt ttgcgtcaaa acttgcctgt ttttaaagga
    34921 gaatatagaa aaacaggcgt tcaacgagcc attttgcgaa cctgttcccg gaaaaatgac
    34981 atatttctgt cacactcttt aattattgat aacaaaagag gtgtgaggaa tgaacaaaac
    35041 gctaatcgcc gcaactgtgg cagggatagt tttactcgct tcaaatgctc aggcacaaac
    35101 cgtaccggaa ggctatcagc tacagcaagt ccttatgatg agccgtcata acttacgtgc
    35161 gccgctggcg aacaatggca gtgtgctgga gcagtcgacg ccgaataaat ggcctgaatg
    35221 ggacgtcccc ggtgggcagc tcaccactaa agggggcgtg ctcgaagtgt atatgggcca
    35281 ttatatgcgt gaatggctgg cagaacaggg gatggtgaaa tcaggggaat gcccgccacc
    35341 ggacaccgtt tatgcctatg ccaatagtct gcaacgcacc gtcgcgaccg cgcagttctt
    35401 tattactggc gcattccctg ggtgtgatat tcctgttcat caccaggaaa aaatgggcac
    35461 tatggacccc acctttaacc cagtgatcac cgatgattcc gccgcattca gtgaacaggc
    35521 ggtggcggca atggagaaag agctcagcaa actccagctt accgacagct acaagctact
    35581 ggaacaaatc gttaactata aagattctcc tgcgtgtaaa gagaagcaac aatgttcgct
    35641 tgtagatggc aaaaatacct ttagcgcgaa gtatcaacaa gaaccgggcg tttccgggcc
    35701 gctgaaagtc ggcaactcgc tggtagatgc gtttactttg caatactacg aaggttttcc
    35761 gatggatcag gtggcctggg gagaaatcaa atctgaccag cagtggaagg tgttgtcgaa
    35821 gctgaaaaac ggttaccagg acagcctgtt tacctcaccg gaagtggcgc gcaatgttgc
    35881 gaaaccgctg gtcagttata tcgacaaagc cctggtcacc gatcgcacca gtgcaccgaa
    35941 aatcacggta ttagttgggc acgactccaa cattgcctct ctgttaacgg cgctggattt
    36001 caaaccgtat cagttgcatg accagaacga acgcacgccg attggcggca aaatcgtttt
    36061 ccagcgttgg catgacagca aagccaatcg cgatttgatg aaaattgaat atgtgtacca
    36121 gagtgcggaa cagttacgta atgccgatgc gttaaccctg caggcacctg cgcagcgcgt
    36181 gacgctggaa ttaagcggtt gcccgataga tgccaacggt ttttgcccaa tggataagtt
    36241 tgatcgcgta ttgaatgaag cggtgaaata acagaaaact cccccgcgtg aagcggggga
    36301 gtcgctggtt aaacgttttt acgttcgatg gtctgttcgc cccaaaaaag cgaatcttta
    36361 tcggtcttag caaaggcttt gactaacact tcatcgctac cttcttccca aatcttttcc
    36421 gccatttttt cgtcgtaccc ggcgacttcg aaaatggcct cggctatttc cggcgacgta
    36481 ttgcgcagag atgcccattc accgacgtga tgagctttcg cttcttgagt tggcatgcgt
    36541 atcctcctgt tgaagattag ctgttaagtt taactgccag acctgcgaca tattcccctt
    36601 gataacgagc aatagacagt tcttcctggc tgggctggcg tgaaccgtca ccgcctgcga
    36661 tggtggttgc gccgtacggc gtaccgccgc gaacctgtga aacgtcaaat aattcctgcg
    36721 ctgcgtagcc aatggggaca atcaccatgc cgtgatgcgc aagggtcgtc caggtggatg
    36781 tgatggtttg ttcctgaccg ccgccagtac cggtggaact aaagacgctc gccagttttc
    36841 cgtatagtgc gccggaagcc cacaggccgc ccgtctggtc gaggaaggta cgcatttgac
    36901 cggacatgtt accaaagcgg gtaggtgtac caaaaataat ggcgtcgtaa tcggccagtt
    36961 cttgcggggt tgcaaccggt gcagtttgcg ttttaccgcc tgctttttca aataattgcg
    37021 gcggcatggt ttccggtaca cgcttaacga caacttctgc gccatccact ttgcttgcac
    37081 cctcagcaac tgcgcgtgcc atcgtttcaa tatgtccgta catggaataa taaagcacca
    37141 gaactttagc catttctaac cactcctcgt gttatctcta ttccgtagcg attcgctacc
    37201 acttatttaa agataagacg tccttttcag agtgcaaatt tcacaaccac ttatttgatt
    37261 tataacaact ttcacaagca cgtaattttg tcgcaaaatg acacattttt atctcatcgc
    37321 gtttttttaa tcataagagc ggcttatgga taattattgg agatgatatc tattctcgct
    37381 aagaagctgt tgcaggatat tactaaacgc gggtctgccc gcgtcagttc actaagctta
    37441 gtcccacgta gcgaaaatat ggcagccgcc atacgccgcg ttaattctat gcaatatgat
    37501 gtctataccc agacggaggt cagtaatggc aaaccatcga ggcggttccg gcaattttgc
    37561 agaagaccgc gaaagagcat cagaagcagg taaaaaaggt ggacagcaca gcgggggtaa
    37621 tttcaaaaat gatccgcagc gcgcatctga agcaggtaaa aaaggaggca agagcagtca
    37681 cggtaaaagc gacaactagc cgggctaatc catgacgaat gtatttttgt ctgaaactcg
    37741 tcaaaaagtc atcaccgccg gttaccccgg cggttttcta tttatggctc ctgatgaaca
    37801 acttctggcg gtggaacgtc aaccaacctt ctgcttaaca acgcattgag taaaatcgcg
    37861 ccaaaggtgg ctgtaccgat ccctcccaac gtaaaaccgc ccagtgtgag agcaaaatca
    37921 cccgcaccca gcactaaggt tactgcgacc ataatcaaat taccgttctg gcttaaatcc
    37981 acactgtttt gtacccagat ccgtgcgcct gcgacggcaa tcagcccgaa cacaacaatt
    38041 gatgcaccac caatcaccgc ggccggaata gtatggatca gcgcgccaaa tttcggcgaa
    38101 aagcccaaca gcatggcgat gacggcagcg gcaacaaaca ccagcgtcga gtagactttt
    38161 gtcacggcca tcacaccaat attctcggcg taggtggtta cgccgctacc gccgacagag
    38221 ccagaaagca tcgtcgccag cccatcgcct acgaatgccc gccccatata cgggtccata
    38281 ttgcgtccgg tcatcccggc gaccgccttg agatgaccta agttttccgc caccagaatc
    38341 accgccacgg gcgcaatcag catcattgcc tgaccattaa aagcaggagt ggaaaaatgt
    38401 ggcagaccga accaggcagc atggctgacg agagtaaaat cgacggcttt tcccagccct
    38461 aaaacgttgg tcatcacgcc atacagcaga caggcgacaa ttaatcctac gagaatcaat
    38521 aatcgctgga tcatgccacg ggtaaatacg gccaccagcc caatacacag caccgtcatt
    38581 accgccatcc agctatcaaa ggccgaagcc gatacatttt tcactgcgat aggcgctaag
    38641 ttcaggccaa tcgccatcac taccgcacct gtcacaaccg gtggcattag tcgttcaatc
    38701 cagcgcgtac cgattttcat caccaccagg ccaatgacgg tataaaccag cccacaggcg
    38761 ataatcccgc ccagcgcgat gctgatattc ggattaattc cctgaccatt aaagcccgtc
    38821 gcggcaatca ccacgccgac aaaagccgcg ctggaaccga gataactggg cacgtgcccg
    38881 ccggtaataa agaaaaacag taacgtgccg atccccgaca ttaaaatgga aagattggga
    38941 tccagcccca tcagaattgg cattaacacc gtcgcgccaa acatcgccac cgcgtgttga
    39001 acgcccatta ctgccgtttg agcaaacggc aatcgttcat ccggcgcgac cacgccgctc
    39061 tctgtagagg tcgattttaa ctgccagtga ggaaaaccga acattgccat cagctgtctc
    39121 cttaaggagg ttaacaagca gggcgcatca gcgcgtgata actgcgatcg aaccacacca
    39181 gcccgtaggg tgtggcgtga cgatgaatcg cttcgatggc gcaaaacaaa atgtcgtggg
    39241 tgccgacgct caccacctgg ctgatacggc agtcaaacga aaccagtgcc tcttccagtt
    39301 gcgggcatcc ggtcacacca gtctgccagc gggcggcggc aaagcggtgt tccatgggcg
    39361 ttttgccgcc aaaaaggttt gaaagcggct cctgcccggc gctaagtgta tttacacaca
    39421 gcgttcgatt ttcattgaat accggccaga cggacgcccc acgattcagg cacaccagta
    39481 atgtgggcgg cgtgtcagtc acgctgcaga cggcgctggc ggtgaacccg gcgcgcccgg
    39541 ctggaccgtc cgtagtgatg atattgaccg ccgcgcccat acaggacatc gcatcgcgaa
    39601 aggtttgttg atcgacaatg ttcattgttt gctccttaca acagcccgca ggcttcttca
    39661 aaggacagac gtggcaggcg cgcaaaaagt ttgctggaat cgccatagcc gatattaatc
    39721 agcagattgc tcttcagcgt gctgcccgca aaaaaggcgt cgtcaacgta ttgacggtca
    39781 aagcccgaca tcgggccggt atccagcccc agcgcacggc aggcgacgat cagataggcc
    39841 gcctgcatgg aactgttgcg aaacgctgtt tcttcggcaa gttgtggact ggaggtaaac
    39901 caactgcggg catcgccgtg aggaaacagc tgtggtaacc gttcataaaa ttcactgtcc
    39961 caggcgacga tagcggtgac gggcgcggtc agggtttttt gcagattgcc gctggaaagc
    40021 gccgggcgca gacgttcttt tccttctgca gtgcggataa acacgatccg tgccggagaa
    40081 cagttagctg atgtcggccc ccatttcatc agggcataaa tctcccgtaa cgtctcatcg
    40141 ctgacgggtg tctcccgcca gccgttgtga gtgcgggcat cggtgaacag ggtgctaagc
    40201 gcacctgggc taacggcttc gttcatagca attccttaca gagcggcttc acggtgatgt
    40261 aacaggctgg caagcccgtt gagtaacaga gcattaaacg tttcgggatc ggtcacgttg
    40321 caggcgtgtc cgccatagcg catcaccatt ttctggctat cgggcagggc ggcatgaagt
    40381 tcactggaac atgctgttgg caccagcaga tcatcactgg cgcagatgat ttgcaccggg
    40441 cagcggatac gatccgcatg gtgactaaag tcagcgcgtt tgagggcgtt aagtcgacgc
    40501 agtaaattat ttttgccttg aaaatgcgcc agtgccagcg cgtcttctgc ctcaaggcga
    40561 ggtgcgcggg ccgccatcca gtcggcggga tagaggaaca acggctgcgc ttccacccat
    40621 gcctgcgcgc cgccgctatg tagtaactgt tcgcgaacct gaaaacagcg gcgcgtatgg
    40681 gcgtttattc gtagccagcc gttaacgctg accagcacag ttaccgacgc gggataatcc
    40741 agcgccagct gcattcccac cagcgcaccg agcgcatggc cgaccaccgc gtaacgctca
    40801 atccctgcgg ctaccagcgc ctgatgcagt tccgctgcca tctgggcgat actgtaatct
    40861 tctgccagcg tgtcgggatt attgccggtg ccgcgctggt cgtaacagac tacctgatac
    40921 tcctgaacca acaccgccag ttgcggtaac cagtaactgc cgctaccgcc aagacccgaa
    40981 atcaacacca ctacgggcgc atcagcataa gggggaggtg agagtgaaag tttcatcgcg
    41041 gcctcacttg gcgatatgcg caattgtggc gatttccacc agcgcgtcag gttttaccag
    41101 tccgcactga atgcagaatc gcgccggttt gtcacccggg aaaaattcgg cgtagatttc
    41161 gttaatcgcg gcgtaatttt tccagtcggt aataaagatg ctgttgaagg tcacatccgc
    41221 cattgtgcca cccgccgtct cgatgacctt gcggatggtt tccagaacgt ggcgggtttg
    41281 cgcctttggg tcatcggcaa acagcacgtt gttatgttga tcaaaagcca gcgtaccgga
    41341 gacatacacc acgccatcag ccagcgtgcc gggaacgaag ggggccagcg gtgcgctgct
    41401 gccagcagga ataattacgg attttggcat cgttaaactc cttaagcgat acgagcaaag
    41461 gacgtggaag aaagcgcgtc gcagaacgtt tcgacgtcgc tgacccagcc aaaaaaggtt
    41521 tcgatattga acaacgcggc tttctgtgca aattccggtc ccgcctggtg tgttgcgtct
    41581 tccagcacca cgccgaaata ctccagaaaa aagccgtcgc gtagcgtcga ttcgacgcag
    41641 acgttggtag cgataccggt gaaaaccaga tggcgtattc cacggctgcg caaaatgctg
    41701 tccagcggcg tattgaagaa accgctgtag cgcggcttcg gcagcacaat atcgccaggc
    41761 tgcggcatca gttcatccac cagttgataa tcccaggagc ctttcgccag caatttcccc
    41821 tgcagctgcg gctgcttacg catggttttc agggcgttcg atttatgaaa attcggtgag
    41881 ccggggccgc cagcctcgac atactgttca tcccagccat tttgaaacca gatgatcaac
    41941 atccctgccg ctcgcgctgc ggtcacggcg gtttgaatgt tggcaatgac cgggcgagtg
    42001 gttgagacat caaacccggc gagatctaag tagccgcctg gcgtggcata agcgttttgc
    42061 atatccacca cgatcagcgc agtttgctgc ggatcgaagg taatggcttc cggtcgtgcg
    42121 gttaaggtcg tcatcatgcc acctcctgag tcagcgcagg gagatgggcg cggcactgca
    42181 tcagtggttg aatgcgctcg ccgaagtttt cgattcccga cagaaaatca tcgaaggtca
    42241 acagcacgcc ttcggcacca ggcacgcttg cgacttcatc taacatgcgc gcgacactgg
    42301 cgtaagaacc gactaacgtc cccatattga tgtttaccgc cgaagtggga tcggccatct
    42361 gacggacgtt ggtgtcagta tcggagcggg tatctttctg actttgttcg gttagccagc
    42421 ttaacgcctc ttcatccgcg cccgctttgt agtgttccca ttttgcatga gcggcatcgt
    42481 cggtttcgtc ggcaatcacc ataaacaaca cataagagcc aacgtcgcgt ccggtttgct
    42541 ctgcggcctg tttcatccgc gcagcggtcg gggcgaaagc cgtgggtgta tttacgcctt
    42601 tgccgaaaca gaagttaaaa tcggcatacc gggcggagaa cgccatgcca gcgtcgcttt
    42661 gcccggcgca gatcactttc atggggacac tcggttgcgg actgacgcga caatcatcca
    42721 tagtgaaaaa atcgccttta aaatcgcttt tccccgagcc ccacaggtcg cgcagcacct
    42781 gaacatattc ggtgagagag tcgtaacgac gggagaaata gtcatcgcca ggccagatcc
    42841 ccatctgctc atactcgggc ttctgccagc cagtcacgag gttgacgcca aaacgcccgc
    42901 cagagatgga gtcgatggtt gcggccatac gggcgacgat tgccggaggt aacgttaagg
    42961 tggcagctgt cgcgtatatc tgaatgcgtg aggtgacggc tgccagcccc gccatcaagg
    43021 tgaacgactc aaggttgtga tcccagaact cagttttgcc gccaaagcca cgcagtttga
    43081 tcatcgacag ggcgaaatcg aaatggtagt gctccgcttt ttgcacaatg gctttattca
    43141 gttcaaatgt cggcatgtac tgcggcgcgt gggtcgaaat gagccagccg ttgttgccaa
    43201 taggtacgaa tacgccaatt ttcatcatca acctctcttc gtctcgtaaa gtgaaagtca
    43261 gacggggcgc tgcatcctgc atatcctttt cagccgcgta atggcttgtt tgcaaagcgg
    43321 atgccagttt ttaaaaagtg aatgttatta atgtgttaat gttaagttgt ctaaaatgcc
    43381 tgaaaaaaag tggactaaac ggtcaaaaca gttgcacata aaacatgcat tcgtgcgcga
    43441 tgagagtgca gaaggtcgag gccgggcggg ggttttgcta tcctgttgcc aatcaacaag
    43501 aggggagagc gcatgacgca aggcgcagtg aaaacaacgg gtaagcgttc gcgcgcagta
    43561 agcgcgaaga aaaaagcgat tcttagcgca gcactggaca ctttttcaca attcggtttt
    43621 cacggcacaa ggctggagca gatcgcggag ttggcgggtg tttcaaaaac caatctgctg
    43681 tattactttc cgtcaaaaga ggcgctgtat atagccgtac tgcggcagat tctcgatatc
    43741 tggctggcac cgttaaaagc gtttcgagaa gatttcgccc cgctggcggc gatcaaagaa
    43801 tacatccgtc tgaagctgga agtttcccgc gattatccgc aggcttcgcg cctgttctgt
    43861 atggagatgc tggcaggtgc accgctgtta atggatgaac tgacgggcga tttgaaggca
    43921 ttaattgatg agaaatcggc gctcattgcc ggttgggtta aaagcggcaa actcgcgccg
    43981 attgacccgc agcatttgat ttttatgatt tgggcttcca ctcaacatta cgccgatttt
    44041 gcccctcagg tggaggcggt gacaggcgcg acgttgcgcg atgaggtgtt tttcaatcaa
    44101 acggttgaaa acgtgcagcg gattattatt gaggggattc gaccacgtta aagatgccgg
    44161 aggaggttgt aacatcctcc ggctacctgt ttaaccgatc gtcattaaac tggcgttacc
    44221 gccagcagca gcggtattca cactcagcga acgctcgata tacagccgtt ccagaaggat
    44281 attgctttcg ccacgggcaa aaccctgcac cgaaacaatt gcaccatccc gcgcggcaac
    44341 ggcttcacac aatgcgcgaa gctgatccga atcaccgtgg aagatcaccg catcaaatgg
    44401 ttgagcggtt atattttccg ctttcgccag ttgaatacgt tcgctgactg ccgatggcaa
    44461 tgccttcact aactgacgat gcagcgcatc atccggccac agcacctggc tacccaccgc
    44521 cagcacggcg gcgagctgag tcagcgcatc ctgttcatca tccgcaatac acaacacgcg
    44581 ctcacgcggc agcagcgtcc aggtgttgcg ttcacccgtc ggccctggca acaatcgttg
    44641 tgttcctgcc tgcgccaact cgccatattg cgtacataac gcctgcaatt ctggacggtt
    44701 tgccgcccat tcccgcagtg catttagcgg ctgagtcaat gcggctttca actgcgcatc
    44761 gaccggatac tccgcatcct gacgcgcgag cgtcactgcc agcgcacttt ccgggcgatt
    44821 cgccagcaga cggtagagat agagcggacc gcctgctttc ggcccggtac cggacaaccc
    44881 ttcgccgccg aacggttgca ccccaaccac tgcgcccacc atattacggt taacgtacag
    44941 gttaccgaca tgcgccgagc cagtgacctg ggcgatggtt tcatcaatgc gcgtatggac
    45001 gccaagcgtc agaccataac cggaagcgtt aatctgctcg atcagcgccg gcagctggtt
    45061 acggttgtaa cgcaccacat gcaacaccgg accaaagacc tctttttgta attcggcaaa
    45121 gtcatccagt tcgatcagcg tcggagcgac aaaggtgccg ctttgccatt cacgggcatc
    45181 ttcgctgttt tcccgcaccg cctggaacac ctgacggcct ttactacgca tggtctgaat
    45241 atgacgctca atattggctt tcgcttcgct atcaatcacc ggaccgatat cggtggtcag
    45301 gcgacccgga ttacccatcc ggcattcggc cattgcgccg cgcagcattt tcagcgtgtg
    45361 gtcggcaatc tcatcttgca ggcacagcac gcgcagtgcc gaacaacgct gacctgcact
    45421 gtcgaacgcc gaggccagca catctattac gacttgttcg gtcagtgctg aagaatcgac
    45481 aatcatcgcg ttcatgccgc cggtttcagc gatgagcgga atagggcgac cctgagcgtc
    45541 caggcggctg gcgatattgc gctgcagtaa cgtagcgact tcggttgaac cggtaaacat
    45601 caccccgcgc acgcgatcat cacccgtcag ttgcgcgccc acggtttcac cccgacctgg
    45661 cagcaattgc accacgcctg gcggtacacc cgcttccagc aaaatggcga tcccttgcgc
    45721 ggcaatcagc ggcgtttgtt ctgccggttt tgccagcacg ctgttacctg ccgccagtgc
    45781 ggcagcgatc tgcccggtga aaatagccag cgggaagttc cacggactga tacataccac
    45841 aggccctaat ggacggtggg tttcgttagc gaaatcatcc cgcacctgtc cggcatagta
    45901 gtggagaaaa tcgaccgctt cgcgcacttc ggcaatggcg ttactgaagg tttttccggc
    45961 ttcacgcacc agaataccaa tcagttgctg catctggctt tccattagca cggcagcgcg
    46021 atgcaaaatc gccgcgcgtt cagccggagg agtggcaaac cagattggcg cgttattaac
    46081 cgcactttcc agcgcctgtt ctacttcacg cggcgtggct tcacgcacaa agcccacaat
    46141 atctttcggt tccgcagggt taataacggg cgacatctca ccagccgcga ccgattgttc
    46201 cagcattggc aaggcctgcc atttttgcag tgcactattg agcagggcag aagagagcga
    46261 ggccagccgg tgttcattgg cgagatccag ccctgccgag ttgtcgcgcc cgtcaccgta
    46321 aagatcgcgc ggcaggggaa ttttcggatg cggtaatcca gtttgccctt cctgttgcgc
    46381 cagtttttct acagcagtga ccggatcggc aaccagttca tccagtggca aagaggtgtc
    46441 agcaatacgg ttaacaaacg aggtgttagc accgttttcc agcaggcgac gcaccagata
    46501 cgccagcagc gtttcatggg taccgacagg agcataaata cgacacggac ggttaagttt
    46561 gccgtcggca actttcccgg tgacctgctc atacagtggc tcgcccatac cgtgcaggca
    46621 ctggaactcg tactgacccg ggtagtagtt ctgacccgcc agttgataaa ttgccgccag
    46681 cgtatgggcg ttatgcgttg cgaactgcgg gtagattaga ttcggcaccg ccagcagctt
    46741 tttcgcacag gcgagatatg aaacgtcggt atacaccttg cgggtataaa ccggataacc
    46801 ttcaaggccg tccatctgcg cgcgcttgat ttcactatcc cagtacgcgc ctttcaccag
    46861 gcgaatcatc agacggcgac ggctgcgggt ggcgagatca atcaggtaat caatcaccgg
    46921 tgggcagcgt ttttgataag cctgaataac aaaaccgatg ccgttccagc ctgccagttc
    46981 cggctcgaaa cagagttttt ccagcagatc gagggagatc tccagacgat cggcctcttc
    47041 ggcatcgatg ttaataccaa tatcgtactg acgcgccagc agggtgagtg atttcagacg
    47101 tgggtaaagc tcttccatta cccggtcata ctgggcgcgg ctgtaacgcg gatgcagcgc
    47161 cgacagcttg attgaaatgc ccggaccttc atagatgcca cgaccattgg acgctttacc
    47221 aatggcgtga atcgcctgct gataggaaac catatacgcc tgtgcatctg cggcggtcag
    47281 cgcggcttcg cccagcatat cgtaggagta acggaaacct ttctcttcca gcttgcgtgc
    47341 attggctaac gcttctgcaa tggtttcacc cgtgacgaac tgctcgccca tcaggcgcat
    47401 cgccatatcc acacctttgc ggatcagcgg ttcaccgctc ttaccgataa tgcggttcag
    47461 cgagcgggag aggctggctt cgttatgggt ggaaaccagt ttgccggtaa acagcagccc
    47521 ccaggtggcg gcattaacaa acaacgacgg gctacgacca atgtgtgact gccagttacc
    47581 gttgctgatt ttgtcgcgaa ttaacgcatc acgggtggct ttgtcgggaa tacgcaacaa
    47641 cgcttctgcc agacacatca gcgccacgcc ttcctgcgat gacaacgaaa actcctgcaa
    47701 taacccctga accatacctg cgcgaccact ggcgtttttt tgattacgca gtttatcggc
    47761 cagttgatac gccagtttgt gcgcctgttc agcaactggc tgcggcaggc gagcttgttc
    47821 cagcagcatc gaaaccgctt cggtttccgg acggcgatag gcagcggtga tcgcggcgcg
    47881 ggaaaccgac tggggcaaaa tttgctcggc aaagtcgagg aaaggctggt gtggttcctc
    47941 tgccggagtc ggtgcttcat cgctctcatt ggcagcgcca gaaagcagcg caggtagctc
    48001 cggcagagta tcgctgtttt ccagttgttc aagataagaa aaaatcgcct gtttaattaa
    48061 ccagtgtggc gtgcgatcga tacgtgtcgc ggcagactta atacgctcac gcgtcgcgtc
    48121 gtccagctta acccccatgg tggtggttcc catgccatta ctcctgttgt tctgaaaggt
    48181 gcaacttaac gttatcgtga aatatccatg atgttgcaac tttgtgcaac tgtgttaaat
    48241 gtgacctgcg tagcaagctt aaaaatgaat gaaatgttaa taaacgaaat cgatatgaca
    48301 gggattaaaa aaataactca gactttttct ctgcggcagt taacattttt gaaaggtgca
    48361 accgcaaaaa atgtgagaga gtgcaacctg atgaaaaata gtgtcgctga gcactaaaat
    48421 ttaatgtaaa tggtgtgtta aatcgattgt gaataaccag cgcttccggc aggatacggt
    48481 cgccctggta aaacataaac tctgttaccc cgttccggtg gcagatataa cggcaagttt
    48541 cgacattgcc gataataatt ttttggagac tttagatggc tattagcaca ccgatgttgg
    48601 tgacattttg tgtctatatc tttggcatga tattgattgg gtttatcgcc tggcgatcaa
    48661 cgaaaaactt cgacgactat attctgggcg gtcgtagtct tgggccattc gtgacggcat
    48721 tatcggcggg agcgtcggat atgagcggct ggctgttaat ggggttgccg ggcgcggttt
    48781 ttctttccgg gatttccgaa agctggatcg ccattggcct gacattaggc gcgtggataa
    48841 actggaagct ggtggccggg cggttgcgtg tgcataccga atacaacaat aacgccttaa
    48901 cattgccgga ttatttcacc gggcgctttg aagataaaag ccgcattttg cgcattatct
    48961 ccgcgctggt tattttgctg ttcttcacca tttattgcgc ttcgggcatt gtggcaggcg
    49021 cgcgtctgtt tgaaagtacc tttggcatga gctacgaaac ggctctgtgg gcgggggctg
    49081 cggcgacgat cctttacacc tttattggcg gtttcctcgc ggtgagctgg actgacactg
    49141 tacaggccag cctgatgatt tttgccctga tcctgacgcc ggttatcgtg atcattagcg
    49201 tcggtgggtt tggcgactcg ctggaagtaa tcaaacaaaa gagcatcgaa aacgtggata
    49261 tgctcaaagg gttgaatttt gtcgccatta tctcgctgat ggggtggggg ctgggttact
    49321 tcgggcagcc gcacatcctg gcgcgtttta tggcggcaga ttctcaccac agcattgtcc
    49381 atgcgcgtcg tatcagtatg acctggatga tcctctgcct ggcaggggcg gtggctgtcg
    49441 gcttctttgg tatcgcttac tttaacgagc acccggcggt agcaggtgcg gtaaaccaga
    49501 acgctgaacg cgtgtttatt gaactggcgc aaattctgtt taacccgtgg attgccggga
    49561 ttttgctgtc ggcgattctg gcggcggtaa tgtcaacgtt aagttgccag ctgctggtat
    49621 gttccagtgc gattaccgaa gatttataca aagcgtttct gcgtaaacag gccagccaga
    49681 aagagctggt gtgggtaggg cgagtgatgg tgctggtggt ggcgctggtg gcgattgcgc
    49741 tggcggcaaa cccggaaaac cgcgtgctgg gcttagtgag ctacgcgtgg gcaggctttg
    49801 gcgcagcgtt tggtccggtg gtgctgttct cggtgatgtg gtcacgcatg acgcgcaacg
    49861 gtgcgctggc ggggatgatc atcggtgcgc tgacggttat cgtctggaaa cagttcggct
    49921 ggctgggtct gtacgaaatt attccgggct ttatcttcgg cagtatcggg attgtagtgt
    49981 ttagtttgct gggtaaagcg ccgtcagcgg cgatgcaaaa acgctttgcc gaggccgatg
    50041 cgcactatca ttcggctccg ccgtcacggt tgcaggaagg gtaagggact tagcctgcgg
    50101 cggttttgtt tggcttcagc agcgggttgc gctcccttaa tgtgcctcgc catataaatt
    50161 gaatggtgca gggagcgcgc agggggcggc c
//
LOCUS       DAZKJR010000017        49843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKJR010000017 DAZKJR010000000
VERSION     DAZKJR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.017"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..921
                     /locus_tag="ACHOWS_001025"
     CDS             <1..921
                     /locus_tag="ACHOWS_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046648.1"
                     /translation="NSLFICCLLIIAYYIYYMTKWNGYIITAFSIISIFIHSYVTCAI
                     RLFRKQKSRLNNTVRYEKLSRKKGFNFWLSLQLIIPGVVQILPMMFLFNQLNFSEGTS
                     DWYEMSILIAITVVIVTIGILPGIIHINERQNGNKMTGIIVVLIFIPVATAALSVWYR
                     PIPNMIANMTMNLSGISDQRTHEYYIERATHPAGMFNGKIWNTRYYKNIPDRFFITGV
                     NTFTLGNIKLICPTAIVKARIESLKFTVNDIDEYEQKGKKLKKTAMKCIPFDKNDIHT
                     WDSPLSEPIYYEKIKQTIDNSMLKILHVVK"
     gene            989..1297
                     /locus_tag="ACHOWS_001026"
     CDS             989..1297
                     /locus_tag="ACHOWS_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFQ0046649.1"
                     /translation="MQSQTALLLLIIPFPVLVIASPESTQLALALKQLDGVKTSLEHA
                     RTSASNDPQARYFFDYLQAQQDISVVEQGIRRYLAPARAQPRAIQPLSANYQQENPDD
                     "
     gene            1290..1523
                     /locus_tag="ACHOWS_001027"
     CDS             1290..1523
                     /locus_tag="ACHOWS_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0046650.1"
                     /translation="MTDSQLAAFQAASLSKPETVSLLVLGLFTSVLLLWCAWTLMVAW
                     QGVCRQRLSWQSFGAVAAHSLLLVLVSFWLVLS"
     gene            1533..1850
                     /locus_tag="ACHOWS_001028"
     CDS             1533..1850
                     /locus_tag="ACHOWS_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFQ0046651.1"
                     /translation="MRWFLLLLFATFNALADLPTMEDPSRGQGKGIMETLQNYGFDIV
                     VLLALAICAVGFLVVANNCIATYSEIQSGRKQWKDLGSMAGVGSILLVVIIWLLNQAS
                     DVL"
     gene            1852..2205
                     /locus_tag="ACHOWS_001029"
     CDS             1852..2205
                     /locus_tag="ACHOWS_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFQ0046652.1"
                     /translation="MATIPFLPDRLNREPVVWRGLTTSELFLALALGLGGGCAFGILL
                     ALITHYWPLIPGSALSGAALLIQGGGRILARAKRGKPDTWLPQALLAWLERHHLRGAQ
                     LIQHSAVWVVRRSPR"
     gene            2202..2861
                     /locus_tag="ACHOWS_001030"
     CDS             2202..2861
                     /locus_tag="ACHOWS_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0046653.1"
                     /translation="MSRFRHALAERDNHILTLRAVIVLLCLCLAGVGYGWYSAPQKLT
                     LWFPPDLRSGSTRPWWEVPPASVYAFTFYIFQQLNRWPDNGEEDYSRNLRSLRAYLTP
                     ACYTRLEHDFQVRRNGGELRDRTRGVYELPGHGYSSLVHEGEKRPFPRVEALSRDSWR
                     VTLDVAVEEYYQNEPVKKALVRYPLQVVRYAIDAEKNPWGLAIDCTDAAPQRLVAEEK
                     P"
     gene            2858..3625
                     /locus_tag="ACHOWS_001031"
     CDS             2858..3625
                     /locus_tag="ACHOWS_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0046654.1"
                     /translation="MKPVLFTLLFSFPAFATEVVQWENIPLPVTLHVGQERIVDVGKP
                     VRIGYPATLEGKVRLQSAGGKVFLLANTAFPATRIQLRDTGNGELILLDIHATQGTST
                     LEPMQISYTPQTPATAKTSVPVTASTEPLPVLLVRYAAQNLYAPLRTVEALPGVTPAP
                     VRLAKRITTLLPQQPVTATPLASWQVNATTVTAIRLQNHSGQRITLDPRELQGQFTAA
                     AFQHDWLGPRGLAEDTTVAYLVTEGPVSRALLPEPKP"
     gene            3622..4917
                     /locus_tag="ACHOWS_001032"
     CDS             3622..4917
                     /locus_tag="ACHOWS_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0046655.1"
                     /translation="MMPRQSLLLKILLPLIVLVTVWLLLRPASKNTQETSLSRTVAAL
                     TPQELRDLGIDGDTPADTVATLVGQMKQYRRELQAIQTNNESQQAREKRLNAQQATLA
                     SQLREDMRRQQDQMRDELVKSQRGLLDKLDRLMAKRPDDIPPGLGLTPGELPADVQPD
                     IRWLEPLDAPPPVDEKLLRVPLKAADKPPPKPDVKVYTLPQNATLTGSVAMTALIGRV
                     PLNGTVSDPYPFKLLIGEDNLTANGVELPDVAGAVVSGIASGDWTLSCVRGQITSMTF
                     VFHDGIIRTVPQGGKSGNKQQEIGWLSDNQGLPCIPGERKSNAKEYLTSQFLLAGSGA
                     AAQAFANGESTTVVEDGSITSAVTGNNGQYVLGQAIGGGLKETADWMKQHYGQMFDAV
                     YVPPGHKVAVHLTEALSIDYEPEGRQVKYQQASTPGGLE"
     gene            4914..5273
                     /locus_tag="ACHOWS_001033"
     CDS             4914..5273
                     /locus_tag="ACHOWS_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFQ0046656.1"
                     /translation="MKFTILILLLLLAGCSTSQEELMPVDPNASMLTLWQQQSGGNAA
                     LLDARAQLRRPPDGPVAQPEADTIRSQFPRLPNPDMVLYVFQHLNSNTPVPGYSTVFP
                     FYTRPQYALPGERLTPP"
     gene            5270..7912
                     /locus_tag="ACHOWS_001034"
     CDS             5270..7912
                     /locus_tag="ACHOWS_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFQ0046657.1"
                     /translation="MNERRGLTEQALNTSYRKGPSFVDWLPWVEFLPESQTLLLEDGR
                     SVGAVFELYPVGTEGRSTAFLEQVRDTVTDALQDAFDEHERAPWVVQFFCQDEDDSEA
                     WLASLASYAAPEIRDSAFTREWLTQMANHMVNLTRDEGLFLDNTVTRTRWRATWRRTR
                     MVVYRWLPGLSPDEPSPEETLNNICERLTAGLKAAGISAIRQNEQDIHRWLVRWFNPA
                     PPAEDKKAFWQQLDLPETEPLPVLADFAERLLASEPRSDAANGVWWLDNRPHKVVVVD
                     RLRKAPPPGLLTGETARGDGINALVDLLPGGTIMNLTVVVYPQDKLEQHISRLADRAI
                     GDNTDSEYTRRDCSEVRSFLKDGHKLYRSTLAFYLSAPDTDILFRQQRQLQSLLLNAG
                     LVPVQEQHEVAPLSHWLRWLPMCFDPLRDPRGLYTRLNFVQHIAGLLPLYGRETGTGH
                     PGISYFNRGGTPLCFDPLNKLDRAQNGHLLLLGPTGAGKSATLNAKIAQLMALHRPRL
                     FIIEAGNSFGLMADYAAAHGLTVNKISLRPGSGVTLPLFADAWRLVEQAVTDAEPPED
                     EESDGDDQRDVLGEMEITARLMITGGEAKEDARLTRPDRAMIRQAIIAAAHTCYEEKR
                     QTLTQDIRDALFALSRDNSLPEARRQRAYEMAESINLFCAGFEGELFNREGQMWPEAD
                     ITLVDLATFAREGYEAQLAISYISLINHINNLGERAQHLARPIVNITDEAHIITVNPL
                     LARFLTKGSKMWRKLGIWLWLATQNLADFPDEAKKLLNMIEWWELLVMPPEEIEQVCR
                     FKALTEEQSQLLRSAVKSPGQYTEGVVLSPRIEALFRVVPPALYLALAMTEKHEKADR
                     MRIMKETGCSEVEAAITLSSVISQ"
     gene            7961..8881
                     /locus_tag="ACHOWS_001035"
     CDS             7961..8881
                     /locus_tag="ACHOWS_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046658.1"
                     /translation="MTAIACWFNREESDSIWAVSDSRITQQNSRLTDHCPKLFSIPVS
                     VIRSSDVYRIHPQKIFEFGFGFAGSTMIGINVKEMLAVSLARLHEIANNNPEQQISTE
                     AFPSLNEIASLAKNIAEQYMSDIGQSFPQAVRIQMIIFGFCWRTQTYKIIKLNNSIEH
                     PANLQIEDIQNLSTGRPVLLGDNLHELNEFIENTRQQFTPNTINWWRTPFIALNNWIN
                     QETVNTIGGYIQMVTAHPLGTRISFLTNLNENIIRTSYAGINTTESFGPTIGGFILMP
                     MDGMSLPDENGWDVGNQVTRTAADRVATNR"
     gene            complement(8884..9108)
                     /locus_tag="ACHOWS_001036"
     CDS             complement(8884..9108)
                     /locus_tag="ACHOWS_001036"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0046659.1"
                     /translation="MKNTSSIQAQFGAHLKKLRLQSGLSQEAFADKCGLDRTYVSGIE
                     RGVRNPTLEVISVLGKGLDMDIKELFNFER"
     gene            complement(9148..9411)
                     /locus_tag="ACHOWS_001037"
     CDS             complement(9148..9411)
                     /locus_tag="ACHOWS_001037"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0046660.1"
                     /translation="MEKTGSIKKVLGKRIRALRREMDLSQEALADKCGIDRTYMSGIE
                     RGVRNPTLVVICAISDGLGMDLSHLFMFDDGAEKDAESTKCDS"
     gene            9519..9794
                     /locus_tag="ACHOWS_001038"
     CDS             9519..9794
                     /locus_tag="ACHOWS_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0046661.1"
                     /translation="MTKKDWHPADIIAALHKRGTSLAKLSREAGLASSTLANALNRPW
                     PKGEWLIAIALGKHPSEIWPERYLNTGNKREPRKPLHLMTTTENSGT"
     gene            10013..10387
                     /locus_tag="ACHOWS_001039"
     CDS             10013..10387
                     /locus_tag="ACHOWS_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0046662.1"
                     /translation="MMTYCARIVAIGLFVADGLADRTLITFDSNGPKDCLDYSLSLEP
                     SFREDDLLILPGDRLLLASHDYLVTAVGKEAQQSLFELGHLTMVFNGSLNACHSGAVH
                     LSGPIPKLHDLYGELLIEEGRP"
     gene            10431..11789
                     /locus_tag="ACHOWS_001040"
     CDS             10431..11789
                     /locus_tag="ACHOWS_001040"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0046663.1"
                     /translation="MELNRSLKARHLTMISVGGVIGAGFFLGAGAAIARTGPSVVLAY
                     LIGGVITLFIMLLLAEMAVARPVSGSFESYARTAFGPWCGFITGWTYWLAFLIGPASE
                     AIAAGTFLNLWFPGVPVWLFCLVIASSLTAINMVGVHFFGEVEFWLSLVKVVALLAFI
                     VAGCYSLGLDNASPASISHPTDSGSFFAGGLPGFLGAMLMVIFSFGGTEAIGTAAGES
                     ENPQRDIPRTLRGTVVRILLLYVGSLTVLLLVLPWQQAGVSSSPYVEASGILGGHMAR
                     HIMNFVVLTAALSCIDTGVYATSRMLHAMASDGYFPAWFARLHPAHKTPDNAILASSL
                     VLFTGVIIAILSPDAYVILAGISGFGFLFTWLMIALSQPTLHRIALHDGTLKYKAPAA
                     RYIQPLTVGLIALIMAGQFFTDGGGITLVTVLIWLALASAYYIFVVRKNRRLSDEKSH
                     AQ"
     gene            11767..12558
                     /locus_tag="ACHOWS_001041"
     CDS             11767..12558
                     /locus_tag="ACHOWS_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HFQ0046664.1"
                     /translation="MKKVMLNELTGREIRELLQSGKELTAIVNFGSCENHADHLPLGP
                     DMFVPEIVACRVAQRVDNTLVLPCTPFGTSVHYNAYPLAVTLRYETNIALAEDIFISL
                     LHHGIRRILVINGHDGNIPAIEIAARNVKARHTQAAIVVVPAWWDLVGHHLGKGFFDS
                     WDNTGLGHGGEGETSVMMALIPELVELKYAQKQIPVEVFADYGVNRIWDIGEISATGA
                     TGDPRCATAEKGQRILACLVDVLCALLRKLDAANWQYALNKDANM"
     gene            complement(12661..13080)
                     /locus_tag="ACHOWS_001042"
     CDS             complement(12661..13080)
                     /locus_tag="ACHOWS_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046665.1"
                     /translation="MANQQAAKWAGVAAIAACLTFVVTTIGLLLAWRSLHQWKPQYKE
                     NSRLLLIEALIAFQKCLITIPKNLDNDPTYQSRKEFLKASTEVELRGQIYLKQHSNEK
                     LKDELANLRSKCAEFVGGKVTKPELSFISAIILLIEV"
     gene            complement(13319..14209)
                     /locus_tag="ACHOWS_001043"
     CDS             complement(13319..14209)
                     /locus_tag="ACHOWS_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0046666.1"
                     /translation="MISQCFDSRFYTFKVESLVYARTLPVNVKFSTKYKQIKASVDEI
                     GLIEPIVVYINDANDKVILDGHLRVEVLKELGITTAHCLISPVEDTYSYNKRVSRLTI
                     VQAQNMLLKAVESGVSVEKLCAVLGVTPDTLIGKLKISDGIAPEVMALLAEKNVPQTI
                     FSVLKKLKFYKQIEMVNTMISINNFTRKFAMSMLHAVAPEHLVAPKKDASRETDIRKN
                     LERLEKEMASVQVETQHLQDEYAENNLRLVIIKNHIERLLANGQVLNWLYDNHQEYLS
                     VLKQISGLDDLNKLGQTIEE"
     gene            complement(14211..15098)
                     /locus_tag="ACHOWS_001044"
     CDS             complement(14211..15098)
                     /locus_tag="ACHOWS_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0046667.1"
                     /translation="MKSFDEQIELVDISDIYIANPRKRNKFIHNEIKENIKTIGLKRP
                     VSVRPVNNENYKYALICGQGRLEAYIEYGETKIPAIIKQVDEETAHLMSLAENIARRN
                     PRAAELMEDIRRMKTSGLTDREIGLRLGYNSSWVNGVITLLSSGEKHLLRAFETGDIP
                     LYLAVEISRSGDEETQELLTVALKEGHIKGGQINKIRRILEQRKKGDKGTISTAFFPG
                     KKQKKLTPEELANVYQENINEQKAILLKSTMVKEKILAVKQIFGYLLKDNDFVTLLHS
                     NNIHDIPSLLDGSDVSGEK"
     gene            complement(15098..16633)
                     /locus_tag="ACHOWS_001045"
     CDS             complement(15098..16633)
                     /locus_tag="ACHOWS_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0046668.1"
                     /translation="MSDIVAGNRIAAQYLRMSTEHQKYSPENQREYIEKYAKEHCITI
                     KYTYDDAGRSGLSLDNRPGLRSLLYDVENGIIKVSLILVYDVSRFGRFQDIEEAAHYS
                     WLLKRNGVRIIYCAEPFSEEQTELFMLGLSYSRYGAATYSKNLSQKVFLGQANLVRRG
                     YYQGGMPGYGIRRLLIDEHTNPKGMLLPGQRKSLQTDRVILIPGPAEEVVIVNKIYDM
                     FIDEGKPEFVIASELNRKNIPAENGLQWSRCKIHQILTNEKYIGNNVYNRTSGRLKTR
                     SVRNPQDQWIRCDGAWDPVVSVEKFEAAKRIIEARAVQYSDDELLDKLRHLLKLRGRL
                     SGIVIDEEELFPSSSIFRSRFGGLVRAYTLIGYHPRHDYSYLEIKKQIAECHLTFMDN
                     LVQDIYRYGGWAEMHQDGSLYINDEFFVSANLVRCQRLSSGKLRWKIKFNVSHYCDVM
                     IFPRLDSTNNSVVDYFMFPAIDVLYNDLTLHENNGFILELYRFDDLSCLENLVKRTGL
                     ESK"
     gene            complement(16657..17604)
                     /locus_tag="ACHOWS_001046"
     CDS             complement(16657..17604)
                     /locus_tag="ACHOWS_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0046669.1"
                     /translation="MRKILFFLLLMIAVLPEVQATCSFYGGVTSEVSGYLNFGNVVIQ
                     RDAQVGSTIATAITGAYNNGDGIAGCKREAWTARWELTQWGTLSGYGNGVYNTNLPGV
                     GLRLSAASTGKTLPYETSYAYNAGGTWVSIPGDGIKGELIKTGDITSGTLTDGVLARA
                     SVVNQFYFANVTLNGMNTVKSEACTVTTPSVDVPMGDHDKSEFSGAGSSTDWVSFDIG
                     LSCDVGARINVRIDATADASASSQGVMLIDSGGASGVGIQLHYRPDDAAVQYGQERFY
                     WQSVYGDEIVQLKARYYQTAGSITPGAANGTATFTMSYK"
     gene            complement(17623..18627)
                     /locus_tag="ACHOWS_001047"
     CDS             complement(17623..18627)
                     /locus_tag="ACHOWS_001047"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0046670.1"
                     /translation="MRVWRGMILLAGILFCQASWAFCTSSVSFSISPGTVVVQRDAVV
                     GEPISDWLVSNQGMAYQDCNYDATPQYVINAGIKNTGSRASVTWNGEAVFNSNVAGVG
                     FVFEGRSSVDSNPLPENWIGIASGQSQVFTLQISHSVGYHPSINDQARIRLIKTGDIT
                     PGTLSGNLGRFIAGTRENGQWSTEIPITFSGGQITTIACSVTTPDVNVPLGQHQKAEF
                     SGPGTGTGWQAFNIGLDCDKSARINVQIDAVQDPSNVAGVMKLDSAPGDMAATGIGVQ
                     LFFAPDNSAVQFGQSRYYYTSPNGGTETVQLKARYYQTAATITAGVANATATFTLTYK
                     "
     gene            complement(18624..21140)
                     /locus_tag="ACHOWS_001048"
     CDS             complement(18624..21140)
                     /locus_tag="ACHOWS_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFQ0046671.1"
                     /translation="MNNPTPRLMIILLTLLLAIVLLSGYLRNAEAREVFNPHALEIDN
                     PGQPVADLSTFAESGGQLPGTYRVTVFVNGEKQSDAQDIAFIDGAKKKLSPQITPAML
                     KAWGVKTDVFPALAALPPDQPLDDIGRYIPMANTELRFSKLQLNVSIPQAAMNASARG
                     YVDPSEWDEGVPAALLNYNLSGSNTWRDGQNGSDDNYYANLQSGINLGAWRLRNYSTW
                     NYDEDNGSHWDSVNTYLQRDIQRLKGQLTLGDSYTPSDIFDSVQFRGAQLASDDNMLP
                     DSLRGFAPVIRGIAQSNAQVTIKQNGYIIYQSYVAPGAFTITDLYPTSGSGDLEVTIK
                     EADGSERTFVQPFSAVPIMQREGRLKYALTAGKYRSGNEDSDEPQFGQVTAIYGLPHA
                     ITVYGGTLYSDDYQSGAAGLGFGLGELGSLSADITAAHTTLNNDETHNGQSYRVQYSK
                     DFQTTDTSFTLAAYRYSTAGFYTFQEANDLRADDDNGWQMTYNKRQRLQLDLSQSIGS
                     YGSFFISGYQQDYWQEDGYERTLSAGWNGNIDGISYSVTWSYSDYPESTQPADQQLAF
                     NIQVPLSRFMPNAWASYSVNTAKHGDTRQQVGLNGTALADNNLSYSLQQSYTNHGVGG
                     SGNINADYKGGQGEITGGYNYDDDTQQVNYGLKGGIVAHPHGITLSQPLGQSLAIVKA
                     PGADDTKVQNNTGVYTDWRGYAVVPYVNPYKKNRIALDTSTLGDEVDTDTAVQTVTPT
                     QGAVVMADFNTRVGRRVLMTLLYRGLPVPFGAEAKLKEGGSGIVGDDGQVYLTGVPEE
                     GDIAVNWNGAQQCVVHYRLPEQENQPSVILVNQECREVAK"
     gene            complement(21152..21832)
                     /locus_tag="ACHOWS_001049"
     CDS             complement(21152..21832)
                     /locus_tag="ACHOWS_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFQ0046672.1"
                     /translation="MNRYADVVLALLLAFLSVQAQAGIVIGGTRVIYDGDKKETAASV
                     RNPEKSGVYLVQSWVDSGEQGGKTPFIVTPPLFRINPGEENMLRIVRTGGNLPQDRES
                     VFWLNVKSIPATDDSHSRTNVLQVVVKSRLKLFYRPAGLEGQPESAYHQLSVAHSGNH
                     LTVSNPTPYYVTLFTLKVDGQEIKEADMVPPKGSVSFTLPSATASAVTWQAISDYGGV
                     SQTESRKL"
     gene            complement(21946..22458)
                     /locus_tag="ACHOWS_001050"
     CDS             complement(21946..22458)
                     /locus_tag="ACHOWS_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0046673.1"
                     /translation="MKKTMLSACFLLVAGNAVANDGTIHFTGSITDQTCTVDSGSQNL
                     NVDLGNVGQTALNGAAGMKASPTRFTLNLSGCPDTVTGANVKFDGTSDGVNQNLLALD
                     SGTGIATGVGIEIADKNGAVIPLHTASMDYPLAAGSNTLDFVARYVSTGTAVTTGTAN
                     GTSQFTINYK"
     gene            complement(22826..23416)
                     /locus_tag="ACHOWS_001051"
     CDS             complement(22826..23416)
                     /locus_tag="ACHOWS_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFQ0046674.1"
                     /translation="MPEKRKHLTRHEVGQILLAADRGIHFERDYCLIQMCFLHGLRVS
                     ELCGMRLSDIDLIGRSVYVRRLKNSLSTQHPLFDNELPALMRWLAVRNRWRDADSDWL
                     FLSQKGGALSRHQVRLLLKRYGELAGVSISAYPHMLRHGCGYALADLGRDTRLIQDYL
                     GHRNIRHTVIYTATNTQRFMNVWGNVRENHTISARM"
     gene            23629..24552
                     /locus_tag="ACHOWS_001052"
     CDS             23629..24552
                     /locus_tag="ACHOWS_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0046675.1"
                     /translation="MRAQYILLLLCAAFPLRAITTPEIAASALTPDCVKYRVVGLCYW
                     LYCTPFGCTVRTSVKVSHFRPDLVVSAYSNTGQNPWAEMSLLSPPLPGVAEGGGDTNP
                     RSASQHSKVRFKNADAIGFPAGDELAKFFTQFGYICSPSSQPFQPYFLSQPDTLAWRS
                     GVPEMTYPEALTPGMREVGQNGDMWGNIYPRAGAINQTHDYKAAAVIAQRAADLVTRT
                     GQPHLYTPLTASSRAGYWPPSPVIEGDSDNHRWQMLTPKKSAACSVFPDGSATDTYAN
                     KLAEDGAYAWTLWRPYKCCPRRGQTFLGSTG"
     gene            24564..25925
                     /locus_tag="ACHOWS_001053"
     CDS             24564..25925
                     /locus_tag="ACHOWS_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFQ0046676.1"
                     /translation="MRALFLVLLFFAGVCVAADDYSFRTRGAVDDLLYYQIGGGEAVM
                     PPPVRRSPSPLSLGLGWNADMMCGNFDIDTTVRNQLNGITDGFQQLMGNVIQSATGAV
                     ASLPAMIIQRANPQLYDLLTNGVLQGRLDFDKSLLNCQKMAEKMTDLAADSAWFSGAK
                     AENYQSLAASDNDAIRTDQKAAKEAAEKGKRWVGGEKRGGKGQLPIKVVHDATAAGWN
                     ILNQQPATSTTSLSSSECDGELCSTWTSPEEAAGWMTRVVGEQTISVAQVTDDPDARS
                     GAQAGIGLHPLIDEEKAKILPLLTDLVNGSQKPGFDNLAKASGGSMQLTRGVVEALRD
                     DPDVAVLTERLAGELALARVTEQALLARRTLLAGMREPNIANVKEAQESLGKTTAQLD
                     EELNQLKLELDFRQALTRNTASQILQRKQQRDQLQGQVVEVPDDSDSRLHNLNNPQPD
                     SPR"
     gene            25922..27424
                     /locus_tag="ACHOWS_001054"
     CDS             25922..27424
                     /locus_tag="ACHOWS_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0046677.1"
                     /translation="MTANSYFEYVLTLLGWLVNNGVWQTLTATGLFAVPLLAKLVALW
                     LKARGQGADEGNVARLLVVWVEHSLYSALLVIFFAGVPFLNVDINTLTYDSQRTKQCG
                     WSVVQPGDSGYAPLINELSGQSASVPVWWYFTHALGKGITSAAVAVLPCKPDLRQLRF
                     EVQHTRLADPALAQELQDFVNDCYGPSRARLKRLSSDISEEQSREVDWVGSSFYLATL
                     GYYDRDHSRSPRAAWPYDASRDDGLSDTGGGGYPACKEWWSDGDTGLKNRLLKQVDTT
                     LWQQLQKLGQSKEEYEEAVLRSLVSPRNMQVSQGGLVYNGFGGSAGDNLSQTQLLSRV
                     GGIIGAGLSSYALFPAFDNVRQALPMVQAFLEMALVICIPVILLFSAWDLKTVITLSF
                     VQFALFFLTFWWELARWLDNWLMQMMYDSDTHSYFNLWGLQNTSDDLIVNIIMGVMFL
                     VLPAFWLGALTWAGIRIGAAVAGGMGSAVGDIRSAGEQVGKMIVSKTRIP"
     gene            complement(27569..27769)
                     /gene="glgS"
                     /locus_tag="ACHOWS_001055"
     CDS             complement(27569..27769)
                     /gene="glgS"
                     /locus_tag="ACHOWS_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFQ0046678.1"
                     /translation="MNRQDINALKNFDFLARSFARMHALGQPVDIDAVTGNMSDEQQA
                     WFRERYDHYRKQAERARVIELR"
     gene            28103..28393
                     /locus_tag="ACHOWS_001056"
     CDS             28103..28393
                     /locus_tag="ACHOWS_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046679.1"
                     /translation="MSLFDKKPSLMPVVIAPDVWQSAVEFPADPQQVEERLGNLLLVV
                     LLTLRTAGTSRKKVTFTIYCQPPQGDMKVPVALPLSLNYHGHYVQVSMAEQA"
     gene            28459..29364
                     /locus_tag="ACHOWS_001057"
     CDS             28459..29364
                     /locus_tag="ACHOWS_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFQ0046680.1"
                     /translation="MDRSNDIYQKVTDQIIAELENGNVPWLRPWRDGEPPFPINALSG
                     RPYHGINVPLLWNSADRQSFASDRWLTFHQVSALGGQVRRGEKSSLAVLYLPRTQEET
                     DALGQPLLDKDGEPKVRHFGIIREFWLFNLSQCDGLPQALSEPFTRPATPVEMAEAIT
                     MNSGVTLRHRRQSKAYYKPATDLVMMPHPQQFESSEAYYATLLHELTHATGHASRLNR
                     PGIAGAERTEALYAAEELVEEMGSAFLCAHCGIQGRLQHASYIGYWLDILRQDKRAVI
                     RASGMARHASEWLLNAVPQPCQLSA"
     gene            29430..29801
                     /locus_tag="ACHOWS_001058"
     CDS             29430..29801
                     /locus_tag="ACHOWS_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046681.1"
                     /translation="MGLDLFFFERKKNAPDESQYARVGYFRKVNPLLKWVDKHVGLVR
                     NQVLLEVTKANLQALLSDLQSLTPDNCGAVYPTIDGFFYGYTDYGEHYWKDVRTVTLW
                     VKETLETFDFERDRLMFYAWW"
     gene            29975..31564
                     /gene="mobH"
                     /locus_tag="ACHOWS_001059"
     CDS             29975..31564
                     /gene="mobH"
                     /locus_tag="ACHOWS_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFQ0046682.1"
                     /translation="MLKWLKRPSQSQPPTPDATLPEGWFAPQSAEQLLATPLRQSCLH
                     QLRQLTPLPDTLFDSWILAAIHRFAALVQLLPASQSHHHSTPGGLLDHSLEVACNAIR
                     LRQGYLLPPEAGAEEQARQASAWTVAVVCVALLHDTGKIAVDMEIHQRDGTRWHSWQG
                     YLAQPYRWQYQSHGRDYHLHPAATAMLINQLIPSPLLDWLAGYPAAFASLIYQLTGHY
                     ERSGALAELVQQADKASVARYLGGDIKTALAQKPSSFPQQLLTALRSLVQNEYRLNNP
                     ECGSDGWLTEDALWLISKTTADRVRAWLLQHGVSSVPENNNRLFDELLTHQLIVANED
                     KAIWRCIIRSDKGWSPVEPLTLLRLTPSVIWGQDDQRPACFDGDVTPVIEAEPAPELS
                     APTQPLPEIADDPRLGDNNNFLQWLKNHTSPSATVNTRNGKVHVVENHLFLISPGIFK
                     TYAAETTGDSGDGWKLAQRMFQESGLALRCNDDSFIWICEVRVPQKKSSQLKGFLMED
                     PKQVLGEKVPVNNPWLKLLRE"
     gene            complement(31620..32273)
                     /locus_tag="ACHOWS_001060"
     CDS             complement(31620..32273)
                     /locus_tag="ACHOWS_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0046683.1"
                     /translation="MKKKLPDQLSRECIIAAMRAYDEGVRHQFSESRLYDVVFEGRSY
                     PPKAIVGIAASNMTGVEFSPEDFSGGIKSKCVKLLTDQGFQIVEKHLGNEQPAISIFP
                     DELPPQSVFTEGAATQVTVNRYERDRKARQAALAWHGCLCKVCGIDMMKVYGEIAKGF
                     IHIHHLIPLSDIKENYSLDPKKDFIPVCPNCHAMLHRRNPPFSPEELKSIIEYHAAL"
     gene            complement(32385..32651)
                     /locus_tag="ACHOWS_001061"
     CDS             complement(32385..32651)
                     /locus_tag="ACHOWS_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005289972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046684.1"
                     /translation="MSPRTHRQLVSVEVMWPAQTLPLPLQQVVEALNQGETPDQIIIR
                     MNQRGLLAWREDASAQDTHDIFQVRLDNQNEARFLCRYVTLPLH"
     gene            complement(32778..33119)
                     /locus_tag="ACHOWS_001062"
     CDS             complement(32778..33119)
                     /locus_tag="ACHOWS_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012776626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0046685.1"
                     /translation="MNVTSPKMLSQAIRDARKQRNLTQRETAASMGMKQSTVSEFENH
                     PEGTSLDTLFKLLAALDLKLQVVSWRGKKTLLMAGSRSGDLWKYEQKGHGAIRTEPPF
                     SLAYHLDFQST"
     gene            complement(33530..34018)
                     /locus_tag="ACHOWS_001063"
     CDS             complement(33530..34018)
                     /locus_tag="ACHOWS_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0046686.1"
                     /translation="MAKKTAPLVPGTNQLLAEFGERLKLARLRRKLTAKRVAERAGMS
                     PMTLRSLESGGTGVTIGAYLSVMQILGLEKDLNKLAAEDEQGRQLQDSRLINKTRVSR
                     PAAISHTNRHTGNVIKNDAFDKQFSTSQEKSIDSEVKTEVVTSASLAALLKPIKKPRK
                     GS"
     gene            complement(34326..35135)
                     /locus_tag="ACHOWS_001064"
     CDS             complement(34326..35135)
                     /locus_tag="ACHOWS_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0046687.1"
                     /translation="MTNIFTLMNPISSATETLLSQINRICTAQGIPFFIAGATAREIL
                     LHHIHSRSIGRRTRDIDIAVFVDSWPRFDALKNAFLAEGAEVVQGNAHRMIVKGIELD
                     IIPFGGVAEGNQIAWPPEREIIMAVDGFAEVFSHTVVVRLDNSENIPFCSLPGLALLK
                     LFAWRDRGHGSTKDATDLFKIICEYGVIENERIYESPVEGERLNWDPIRMGAFLLGSD
                     IATISEHSSLQDLLHLDKERLIDVIVRQHDVGDAGIEQIINDFWDGMASDG"
     gene            complement(35089..36087)
                     /locus_tag="ACHOWS_001065"
     CDS             complement(35089..36087)
                     /locus_tag="ACHOWS_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HFQ0046688.1"
                     /translation="MKEEQLLSDAIENLPKGFHLQYEHSGRHGKHDAWGKLTGPDGES
                     VIFALEVKFIHRKESLMAVREQMVRFPADFPVLLVCNRLTSALAEYCADSQINFIDTA
                     GNARIQVPGLYVRIEGRYEKKPVSASSRFAEGVMKLLFVLLSSPETLNETYRSLAGKA
                     GISLGMVSKAFDYLEAQRYFRKSKKGRRLINEGELEALWLKDYATALRPKLDSLSLSA
                     PVSWDELTLASGEYWSGEIAAAKLSGGYLIPENGVIFTSHPLLQRRKELGLKPVPGGK
                     LQLIAGFWGNFVLNHKAEAMLCVAELLASGDDRNWETARIINDKYLHLNESDLFSY"
     gene            complement(36715..37962)
                     /locus_tag="ACHOWS_001066"
                     /pseudo
     CDS             complement(36715..37962)
                     /locus_tag="ACHOWS_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(38185..38269)
                     /locus_tag="ACHOWS_001067"
     tRNA            complement(38185..38269)
                     /locus_tag="ACHOWS_001067"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38233..38235),aa:Leu,seq:caa)
     gene            38465..39484
                     /gene="ahr"
                     /locus_tag="ACHOWS_001068"
     CDS             38465..39484
                     /gene="ahr"
                     /locus_tag="ACHOWS_001068"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFQ0046689.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTILNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            39614..41116
                     /gene="yjgR"
                     /locus_tag="ACHOWS_001069"
     CDS             39614..41116
                     /gene="yjgR"
                     /locus_tag="ACHOWS_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFQ0046690.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLPWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(41235..42317)
                     /gene="lptG"
                     /locus_tag="ACHOWS_001070"
     CDS             complement(41235..42317)
                     /gene="lptG"
                     /locus_tag="ACHOWS_001070"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFQ0046691.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(42317..43417)
                     /gene="lptF"
                     /locus_tag="ACHOWS_001071"
     CDS             complement(42317..43417)
                     /gene="lptF"
                     /locus_tag="ACHOWS_001071"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFQ0046692.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(43480..43530)
                     /gene="ytgA"
                     /locus_tag="ACHOWS_001072"
     CDS             complement(43480..43530)
                     /gene="ytgA"
                     /locus_tag="ACHOWS_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFQ0046693.1"
                     /translation="MSIIILATISTTQENK"
     gene            43684..45195
                     /gene="pepA"
                     /locus_tag="ACHOWS_001073"
     CDS             43684..45195
                     /gene="pepA"
                     /locus_tag="ACHOWS_001073"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFQ0046694.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYRGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            45329..45772
                     /gene="holC"
                     /locus_tag="ACHOWS_001074"
     CDS             45329..45772
                     /gene="holC"
                     /locus_tag="ACHOWS_001074"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFQ0046695.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            45772..48627
                     /gene="valS"
                     /locus_tag="ACHOWS_001075"
     CDS             45772..48627
                     /gene="valS"
                     /locus_tag="ACHOWS_001075"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0046696.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLSADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(48681..>49843)
                     /gene="yjgN"
                     /locus_tag="ACHOWS_001076"
     CDS             complement(48681..>49843)
                     /gene="yjgN"
                     /locus_tag="ACHOWS_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFQ0046697.1"
                     /translation="HSFVFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLYA
                     NMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCVLTLLLLVFLIFM
                     AAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLPANS
                     SSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYA
                     ILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYF
                     GIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAY
                     PLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT        11831 a        13633 c        12904 g        11475 t
ORIGIN      
        1 aatagcttat ttatatgctg cctgctaatc attgcttatt acatttatta catgacaaaa
       61 tggaatggct acataattac agcctttagt attatttcca tatttattca cagctatgtt
      121 acatgtgcga ttcggctatt tagaaaacaa aaatctcgct taaataatac agtgcgatac
      181 gaaaaactat ccagaaagaa aggattcaac ttctggttat ccttacaact tataatacct
      241 ggagtagtac aaatattacc aatgatgttt ctgtttaacc aacttaattt cagtgagggt
      301 actagtgact ggtatgagat gagcatactc attgccataa ctgtagttat tgttacaatt
      361 ggcattctgc ctggtatcat acatattaat gaacggcaaa acggcaataa aatgacagga
      421 attattgttg ttttaatatt tatacctgtc gcaaccgctg cacttagtgt ttggtacaga
      481 cctattccca acatgatagc taatatgaca atgaatcttt cagggattag tgaccagaga
      541 acccatgaat actatattga aagagcaact cacccagcag gtatgtttaa tggtaaaata
      601 tggaatactc gatactataa aaacatccct gatagattct tcattactgg agttaacaca
      661 tttactttag ggaatataaa actcatctgc ccaacagcaa tagtcaaagc aagaatagaa
      721 agtttgaaat tcactgtaaa tgacattgac gagtacgaac aaaaagggaa aaaactaaaa
      781 aagacagcga tgaaatgtat tccctttgat aaaaatgata tacatacctg ggactcgcca
      841 ttatctgaac caatttatta cgagaaaatc aaacagacca ttgacaactc tatgctaaaa
      901 attcttcatg tagtgaaatg attttttagc tgcttcacct ctgaagctct cctactttgc
      961 taatcattga gtataaggag agcttcaaat gcagtcccag acagcactat tactgttaat
     1021 aatacctttc ccggtactgg taatcgcctc cccggaatcc acccagctcg ccctcgcgct
     1081 gaagcaactc gacggcgtta aaacctcgct ggagcacgcc cggaccagcg ccagcaacga
     1141 tccgcaggcg cgctacttct tcgactactt acaggcgcag caggacatct ctgtcgttga
     1201 acaaggcatt cgccgctatc ttgccccggc tagagcacag ccgcgtgcta ttcagcccct
     1261 gagcgccaac taccagcagg agaatcccga tgactgactc gcaactcgcc gcctttcagg
     1321 ccgccagcct gagcaaaccg gagaccgtca gcctgctggt gctcggcctg tttaccagcg
     1381 tcctgctgct gtggtgtgcc tggacgctaa tggtcgcctg gcagggcgtc tgccgccagc
     1441 gtctgagctg gcagagcttt ggcgcggtgg cggcccattc actgttactg gtgctagtca
     1501 gcttctggct ggtactgagc tgaggaggca tcatgcgctg gtttctgtta ctgctgttcg
     1561 ccacatttaa cgccctcgcc gacctgccga cgatggaaga tccgtcacgc ggtcagggca
     1621 agggcattat ggagacgctg caaaactacg gttttgacat cgtggtgctg ctggcgctgg
     1681 ccatctgtgc cgtcggtttt ctggtagtgg cgaacaactg tattgctacc tattcggaga
     1741 tccagagcgg acgcaagcag tggaaagatc tgggctcgat ggcaggcgtc ggcagcattc
     1801 ttctggtggt catcatctgg ctgctcaacc aggcttcgga cgtcctgtga tatggcaacc
     1861 atcccctttc tccccgaccg cctcaaccgc gagcccgtgg tctggcgcgg cctcaccacc
     1921 agcgaactgt ttctggcgct ggctctcggt cttggcggcg gctgtgcgtt cggcattctg
     1981 ctggccctga taacccacta ctggccgttg atccccggtt cggcactttc tggcgcggca
     2041 ttactgattc agggtggtgg gcggattctg gcccgcgcaa aacgcggtaa gcccgacacc
     2101 tggctgccgc aggcgctcct cgcctggctg gagcgtcatc atcttcgcgg cgcacaactg
     2161 atccagcaca gcgccgtgtg ggtggtgcgc aggagtccac gatgagccgc tttcgccatg
     2221 cgctggctga gcgcgataac catatcctga cgctgcgggc ggtgattgtt ctgctgtgcc
     2281 tgtgtctggc gggcgtcggc tacggctggt acagcgcgcc gcaaaagctc accctctggt
     2341 tcccgccgga tctgcgcagc ggcagcaccc gcccgtggtg ggaagtgcct cccgccagcg
     2401 tctacgcctt caccttttat atcttccagc agcttaaccg ctggccggat aacggcgagg
     2461 aggactattc ccgtaatctt cgttcgctgc gtgcctacct gacgcccgcc tgttacaccc
     2521 ggctggagca cgattttcag gtgcgtcgca acggcggtga actgcgcgac cgcacgcgcg
     2581 gcgtctatga actgcccggc cacggctaca gcagcctggt gcatgagggc gagaagcgcc
     2641 ccttcccgcg cgtggaggcg ctgagccggg attcatggcg ggtaacgctg gatgtggcgg
     2701 tggaagagta ctaccagaac gagccggtga aaaaagcgct ggtgcgctac ccgttgcagg
     2761 tggtgcgcta cgccatcgat gcggagaaaa atccgtgggg actggcgatc gactgtaccg
     2821 atgccgcgcc gcagcgtctg gtggcggagg agaaaccatg aagccagtcc tctttacttt
     2881 attgttcagt ttccccgcct tcgccacgga agtggtgcag tgggaaaaca tcccgctgcc
     2941 cgtcacgctg catgtcgggc aggagcgaat tgttgacgtg ggtaaaccgg tgcgcattgg
     3001 ctatcccgcc acgctggaag gcaaagtgcg tctgcaaagc gcaggcggca aggtcttcct
     3061 gctggcgaac acggcgttcc cggccacccg catccagtta cgtgataccg gcaacggtga
     3121 gctgatcctg ctggatattc atgccaccca gggcacttcg acacttgaac caatgcaaat
     3181 tagctatacg ccgcagacgc ctgccacagc aaaaacatcc gtcccggtga cagcatcaac
     3241 agaacctctg cccgtcttgc tggtgcgcta cgccgcgcaa aatctgtatg cgccgctgcg
     3301 caccgtcgaa gccctgcccg gcgtcacacc tgcgcccgtc cggctggcga aacgcatcac
     3361 cactttactg ccgcagcagc cggttaccgc caccccgctg gcgtcatggc aggtcaacgc
     3421 caccaccgtc accgccatcc gcctgcaaaa tcacagcgga cagcgcatca cgctcgatcc
     3481 gcgcgagctt caggggcagt tcaccgctgc cgcgttccag catgactggc tcggcccgcg
     3541 tggtctggca gaagatacca ccgtggcgta cctggtgacg gaaggtccgg tttcccgcgc
     3601 cctgctgccg gagcccaaac catgatgccg cgccagagcc tgctgctgaa aatcctgctg
     3661 ccgctcatcg tactggtaac ggtatggctg ctgctgcgcc cggccagcaa aaacacgcag
     3721 gagacttccc tgtcccgcac cgtggcggcc ttaacgccgc aggaactgcg cgatctgggc
     3781 atcgacggcg acacgcccgc cgacaccgtc gccacgctgg tcggccagat gaaacagtac
     3841 cgccgcgaac ttcaggcaat ccagacaaac aacgaaagcc agcaggcgcg cgaaaaacgg
     3901 cttaacgcac agcaggccac gctggcctct cagcttcggg aagacatgcg ccgccagcag
     3961 gaccagatgc gcgatgaact ggtgaaaagc cagcgcgggc tgctggataa gctcgaccgt
     4021 ctgatggcca agcgcccgga cgatatcccg cccggtctgg ggctgacgcc cggcgaacta
     4081 cctgccgacg ttcaacccga tatccgctgg ctggagccgc tggatgcgcc tccacctgtg
     4141 gatgaaaagc tgctgcgcgt ccctctgaaa gcggccgaca aaccaccgcc gaaacctgac
     4201 gtgaaggtct acaccctgcc gcagaacgcc acgctgaccg gatcggtggc gatgacggcg
     4261 ctgattggcc gcgtgccgct taacggcacc gtcagcgatc cctacccgtt taaacttctg
     4321 attggcgagg acaacctcac cgccaatggc gttgagctgc cggacgtggc gggcgcggtg
     4381 gtctccggca tcgcctcggg agactggacg ctctcctgtg tgcgcgggca aatcacctcg
     4441 atgacctttg tgtttcacga cggcatcatc cgcaccgtcc cgcagggcgg caaatccggc
     4501 aacaaacagc aggagatcgg ctggctttcg gataaccagg ggctgccatg tattcccggc
     4561 gagcgtaaaa gcaatgcgaa ggagtacctg acctcgcagt tcctgctggc gggttccggt
     4621 gcggcggcgc aggccttcgc caatggcgaa agcaccacag tggtcgagga cggcagcatc
     4681 accagcgcag tgacgggcaa caacggtcag tacgtgctcg gccaggctat tggcggcggg
     4741 ctgaaagaga ccgccgactg gatgaaacag cactacggcc agatgttcga cgccgtgtac
     4801 gtgccgccgg gccacaaggt ggcggtgcat ctcaccgaag cgctgtctat cgactacgag
     4861 ccggaaggcc gtcaggtgaa ataccagcag gccagcacac cgggaggtct ggaatgaaat
     4921 ttaccatcct tatacttctg ttgttactgg ccggatgcag cacctcgcag gaggaactga
     4981 tgcctgtcga ccccaatgcc agcatgctga cgctgtggca gcagcaaagc ggcggcaacg
     5041 cggctctgct ggatgcccgc gcccagctac gccgcccgcc agatggtccg gtggcgcagc
     5101 ctgaggcaga cactatccgc agccagtttc cgcgcctgcc caacccggat atggtgcttt
     5161 acgtcttcca gcacctcaac agcaacacgc cggtacccgg ctacagcacc gttttcccct
     5221 tctatacccg tccgcagtat gcgttgccgg gcgaacgcct gacgccgcca tgaatgaacg
     5281 gcgcggcctg actgaacagg cgctgaatac cagctaccgc aagggcccgt catttgtcga
     5341 ctggctgccg tgggtggagt ttttgccgga gtcgcagacg ctgctgctgg aagatggccg
     5401 ctcggtaggt gccgtatttg agctatatcc cgttggcacc gaaggccgct caacggcgtt
     5461 tctggaacag gtccgcgata ccgtcaccga tgccttacag gatgcgtttg atgagcatga
     5521 gcgcgcaccg tgggtggtac agtttttctg tcaggatgag gacgacagcg aggcgtggct
     5581 ggcatcactt gcctcttacg ccgcaccgga aatccgcgac tccgccttta cccgcgagtg
     5641 gctcacacag atggcaaacc atatggtgaa tctcacccgc gacgagggac tgtttctcga
     5701 taacaccgtc acccgtaccc gctggcgggc gacgtggcgg cgcacgcgga tggtggtgta
     5761 ccgatggctt cctggacttt caccggatga accgtcgccg gaagagacgc tgaataatat
     5821 ctgcgaacgc cttaccgccg ggctaaaagc ggcgggtatc agcgctatcc gccagaatga
     5881 gcaggacatt caccgctggc tggtgcgctg gtttaatccc gcgccgcctg ctgaggacaa
     5941 aaaagccttc tggcagcagc ttgacctgcc ggaaactgaa ccgctgccgg tcctggccga
     6001 tttcgccgaa cgcctgctgg cgagtgaacc ccgttcggat gcggcaaacg gcgtctggtg
     6061 gctggataac cgcccgcata aagtggtggt ggttgaccgc ctgcgcaaag cgccgccgcc
     6121 cggcctgctg accggggaaa ccgcgcgcgg agacggcatt aacgccctgg tcgacctgct
     6181 gcctggcgga accatcatga acctgaccgt ggtggtgtat ccgcaggaca agctggagca
     6241 acacatcagc cgcctggccg accgggcgat tggcgacaat accgattcgg aatacacccg
     6301 ccgcgactgc agcgaggtgc gaagcttcct gaaggacggc cataaactct accgcagcac
     6361 gctggcgttt tatctcagcg cgccggacac cgatatcctg ttccgccagc agcgtcagct
     6421 acagagcctg ctgctcaacg ccgggctggt tccggtgcag gagcagcacg aagtcgcccc
     6481 cttaagccac tggctgcgct ggctgccgat gtgcttcgat cccctgcgcg acccgcgcgg
     6541 gctgtatacc cggctcaact ttgtgcagca tatcgccggg ctgctgccgc tgtacggacg
     6601 ggaaaccggc accgggcatc cggggatcag ctacttcaac cggggcggca cgccgctgtg
     6661 cttcgatccg ctgaacaagc tggatcgtgc ccagaacgga caccttttac ttcttgggcc
     6721 aaccggagcg ggcaaatccg ccaccctcaa cgcgaaaatc gcgcaactga tggcgctaca
     6781 tcgtccgcgc ctgtttatca tcgaggccgg aaactcattt ggcctgatgg ccgactacgc
     6841 cgccgcccac ggcctgacgg tgaataaaat cagcctgcgc cccggttctg gcgtcaccct
     6901 gcccctgttc gccgatgcgt ggcgactggt ggaacaggca gttaccgacg ccgaaccgcc
     6961 agaggacgaa gagagcgacg gcgatgacca gcgcgacgtg ctgggtgaaa tggagatcac
     7021 cgcccggttg atgattaccg gtggtgaagc gaaagaggat gcgcgcctca cccggccgga
     7081 tcgggcgatg atccgccagg cgattatcgc tgccgcgcac acctgctacg aggaaaaacg
     7141 acagacgctg acccaggata tccgtgacgc actgttcgcc ctttcccgcg acaacagtct
     7201 gccggaagcc cgccgccaga gggcgtatga aatggcggaa tccatcaacc tgttctgcgc
     7261 cgggtttgaa ggcgaactgt ttaaccgtga aggccagatg tggccggagg cggatatcac
     7321 cctagtggat ctcgccacct ttgcccgcga aggctatgaa gcgcagcttg ccatcagcta
     7381 tatctcgctg attaaccata tcaataacct cggcgagcgc gcccagcatc tggcgaggcc
     7441 tatcgtcaat atcaccgatg aggcgcatat catcactgtt aatcccttat tggcgcgctt
     7501 tctcaccaaa ggctcgaaga tgtggcgcaa gctcggtatc tggctgtggc tcgccaccca
     7561 gaacctggcg gattttcctg atgaggcgaa gaagctgctc aatatgatcg aatggtggga
     7621 actactggtg atgccgccgg aagagatcga gcaggtctgc cggtttaaag cgcttacgga
     7681 agaacagagc caactgctgc gctcagcggt aaaatcaccg gggcagtata ccgagggcgt
     7741 ggtgctttca ccgcgtatag aggcactgtt tcgggtggtg ccgccagcgc tctatctggc
     7801 gctggcgatg acagagaaac atgaaaaggc cgataggatg cggattatga aggagacagg
     7861 gtgtagtgaa gtggaggcgg cgataaccct aagctcggta atctcgcaat aaaaatatgc
     7921 taagctattc gcattaatgt taaataactg gaaaaacctt atgaccgcaa ttgcatgttg
     7981 gtttaataga gaggaaagcg atagtatttg ggcagtcagc gactcaagaa tcactcagca
     8041 aaatagcaga ctaactgacc attgtccgaa attattctca atccccgtat cagttattcg
     8101 ctcttcggac gtttatcgaa ttcacccgca aaaaatattc gaatttggct tcggatttgc
     8161 aggaagtaca atgattggaa ttaatgtcaa agaaatgctt gcagtatcat tagcaagact
     8221 tcatgagatt gcgaacaata atccagaaca acaaatatcg accgaagcat tcccaagtct
     8281 taatgaaata gcttcattag caaaaaatat tgccgagcaa tatatgtctg atatcggcca
     8341 atcctttccc caagcagtaa gaatacagat gataattttt ggtttttgct ggagaacaca
     8401 aacctacaaa attataaaac tcaataactc tattgagcac ccagccaacc tccagattga
     8461 agatattcaa aatttatcaa caggcagacc tgttttatta ggtgataact tgcatgaact
     8521 taatgagttt atagagaata ccaggcaaca atttactcct aacacaataa attggtggcg
     8581 taccccgttt attgctttga ataattggat taaccaagaa acagtcaata ctattggtgg
     8641 atacatccaa atggtaaccg cgcacccact tggtactcga atatcatttt tgaccaattt
     8701 gaatgaaaat attatcagaa caagctatgc aggaattaac actactgagt catttggacc
     8761 aactattggt ggttttatcc taatgccaat ggatgggatg tcactcccag atgaaaacgg
     8821 ctgggatgtt ggaaatcaag ttacccgtac tgctgcggat agagttgcta ctaatagata
     8881 aacctatctt tcaaagttga ataattcttt gatgtccatg tccaaccctt ttcctaaaac
     8941 acttatcacc tcaagggttg gattccttac tccccgctca ataccgctca cataagtacg
     9001 atcgagacca cacttatccg cgaatgcttc ctgagacagg cccgactgga gtctgagttt
     9061 tttgagatgt gcgccaaact gcgcctgaat actggatgta tttttcatcc agataaaatg
     9121 aacttacgtt gtctatcaga ccacggacta tgagtcacac ttcgttgatt cggcgtcctt
     9181 ttctgcgcca tcgtcaaaca taaataaatg gctaagatcc atccccagac cgtccgagat
     9241 tgcacaaatg acaacaagcg tggggttcct tactcctcgc tcaattccac tcatataggt
     9301 gcgatcgata ccgcatttgt cggctaaagc ttcctgggat aggtccatct cacggcgtag
     9361 agccctgatt cttttaccca gtaccttttt gattgatccc gtcttttcca tctagccagc
     9421 atcaatcttc gttgattatc agtccacgga ctatgagtca cataaaaagc gtgctagcat
     9481 acattcgtga ctcatagtca acaatagagg taaacggaat gactaaaaaa gactggcacc
     9541 ccgccgatat cattgccgcc ttacacaagc gcggcaccag tctggcgaaa ctctcgcggg
     9601 aagcgggact ggcctcgtca acgctggcga atgccctgaa ccgaccgtgg ccgaaaggtg
     9661 agtggctgat tgccattgcg ctgggaaagc atccgtcaga aatctggccg gaacgttacc
     9721 tcaatacggg aaataaacgt gaaccccgta aaccgcttca cctcatgaca acaacagaaa
     9781 actccgggac ataacgaccc gcgctgtgag tggcaggacg cattaatcaa cgacacctga
     9841 caggagagtt gtttatgcac accgaaccac acccttgctc catcgctctt acaaccggag
     9901 gccaccttca cccggcctcc cggactgaag gctttattca cctattcagg ctacatgcct
     9961 gctcacctac accaggccgt actctcatgc ccgacaaacg ggagaacgag ccatgatgac
    10021 ctactgcgca cgcatcgttg ctatcgggct gtttgtcgcc gacggactgg cagacaggac
    10081 gcttatcacc tttgacagca acggaccgaa agactgcctg gactattccc tgtcgctgga
    10141 accctcattc cgcgaagacg acctgctgat cctgcccggc gatcggctgc tgctggcgag
    10201 ccatgactac ctggtgaccg ccgtgggtaa agaggcacag cagtctttgt ttgaacttgg
    10261 ccacctgacc atggtgttta acggcagcct gaatgcctgc cattcgggag ccgttcatct
    10321 ctccggccca atccctaaac ttcacgacct gtacggtgaa ctgctgattg aggagggacg
    10381 gccataagcg ctgccttgaa tccatccaca acaacaaaag agaagacatt atggaactca
    10441 acagaagtct gaaagcccgt cacctgacga tgatttccgt gggaggcgtg attggtgccg
    10501 gatttttcct cggcgcaggc gcggccattg cccgtaccgg gccgtcggtg gtgctggcct
    10561 accttatcgg cggtgtcatc accctgttca ttatgctgtt gcttgccgaa atggcggtcg
    10621 ccaggcccgt ctccggttcc tttgaatcct atgcccgcac cgcctttggc ccatggtgtg
    10681 gctttatcac cgggtggacc tactggctgg ccttcctgat tggcccggcg tcggaggcca
    10741 tcgctgcagg caccttcctg aacctgtggt ttcccggagt ccccgtctgg ctgttttgcc
    10801 tggtgatcgc ttcgtcgctg actgccatca acatggtcgg cgttcatttt ttcggtgaag
    10861 tggagttctg gctgagcctg gtgaaagtcg tggcgctgct ggcctttatc gtcgcgggct
    10921 gctacagcct gggactggat aacgcctcac ccgccagcat cagtcatccg accgacagcg
    10981 gcagcttttt tgctggcggg ctgcccggat ttctcggcgc gatgttaatg gtgattttct
    11041 ctttcggcgg gacagaggcc atcggcaccg ccgcgggtga aagcgagaac ccacagcgtg
    11101 atattcctcg tacgctgcgt ggcaccgtgg tgcggatcct gttgctgtat gtcggctcat
    11161 tgaccgttct gctgctcgtt ctcccctggc agcaggcagg tgtcagcagc agcccgtatg
    11221 tcgaagcttc cggcattctc ggtggccata tggcgcggca tatcatgaac tttgtggtgc
    11281 tgaccgccgc gctctcctgc atcgataccg gggtttacgc cacctcgcgg atgctgcacg
    11341 ccatggcgtc tgacggctat ttcccggcct ggtttgcccg cctgcacccg gcacataaaa
    11401 caccggataa cgccattctt gccagcagcc tggtgctgtt taccggcgtc atcatcgcca
    11461 ttctctcgcc agatgcgtat gtcatcctcg ccgggatttc cggattcggc tttctgttca
    11521 cctggctgat gattgcctta agccagccca ccctgcaccg aatagccctg cacgacggaa
    11581 cgctgaaata caaggctccg gccgcccgct acatccagcc actcaccgtg gggctgattg
    11641 cgctgatcat ggccgggcaa tttttcaccg acggcggcgg cattaccctg gttaccgtcc
    11701 tgatctggct ggccctcgcc agcgcgtact acatctttgt cgtccgaaaa aacaggaggc
    11761 tgtccgatga aaaaagtcat gctcaatgaa ctgactggcc gggaaatccg cgagctgttg
    11821 cagtctggta aggaactcac tgctatcgtc aacttcggca gttgtgagaa ccacgccgat
    11881 cacctgccac tcggccccga tatgtttgta cccgaaatcg tcgcctgccg ggtggcgcag
    11941 cgggtggata atacccttgt gctgccctgc acgccgttcg gcacatccgt tcactacaat
    12001 gcctatccac tggcggtcac cctacgctac gagaccaata tcgcgctggc ggaagatatc
    12061 ttcatcagcc tgctgcacca cggtattcgc cggatcctgg tgattaacgg ccatgacggt
    12121 aatattccgg cgattgaaat cgccgcccgc aatgtgaagg cccgacatac gcaggcagcg
    12181 attgtcgtgg ttccggcgtg gtgggatctg gtgggacatc atctcgggaa gggatttttc
    12241 gactcatggg ataacaccgg actaggacac ggcggcgagg gcgaaacgtc cgtcatgatg
    12301 gcgctgattc cggagctggt ggaactgaaa tatgcgcaga aacagatccc ggtggaggtg
    12361 ttcgctgatt atggcgtgaa ccggatctgg gatatcgggg aaatcagcgc caccggagca
    12421 accggcgatc cgcgctgcgc cacggcggaa aaaggccagc ggatcctcgc ctgtctggtg
    12481 gatgtgcttt gcgcgttgct gagaaagctg gatgcagcta actggcaata tgcgttaaat
    12541 aaagacgcaa acatgtaatc gatatgctcg ggcaacctgt ggagtggctt ttgcctttcc
    12601 acaggttacc cggaagcgat atcccaaccg ggtaaccgat tggtgttagc ccagaatcta
    12661 ttatacttca atgagtaata ttattgctga gatgaatgac agttcaggtt tagtcacctt
    12721 accaccaaca aattctgcac atttactcct gagatttgct aattcatctt ttaatttttc
    12781 gttgctgtgt tgcttaagat atatttgtcc tcttagctca acttcagtac ttgccttcaa
    12841 aaattccttt cggctttgat aggtgggatc gttatcgaga ttttttggta tagttattaa
    12901 acatttctga aatgcgatta aagcttcgat taggagaagt ctagagtttt ctttgtattg
    12961 aggcttccac tgatgcaacg accgccaagc aagtagtaaa cctatcgttg tcacaacaaa
    13021 cgtcaaacag gcagcaatag cagcaacccc cgcccatttt gcagcttgtt gattagccac
    13081 caatgtgctc aagcttatga aatccgcatc tatatgcact atttttacct cagtaagaaa
    13141 agtggatgta aatctacttt ttcatttttg aaacagcaac ttgattttaa gccatcgtcc
    13201 tttgagcaag attttccgca ccagttgaat cacgtcaacc accagcacgc cctcctctct
    13261 cagtgcatcc catcataatg gcaatctgta aaacagcagt ccttgcttta aaaatgtgtc
    13321 attcctcaat tgtctggcca agcttattca aatcatccag ccccgagatt tgcttgagca
    13381 cagaaaggta ctcctgatgg ttatcataaa gccagttaag tacctgaccg ttcgccagca
    13441 gacgttcaat gtgattttta atgataacca gccgaaggtt attctcagca tattcatcct
    13501 gaagatgctg cgtttccacc tgaacggaag ccatttcttt ttccagtctt tcgagatttt
    13561 ttctgatatc tgtttctctg ctggcatctt ttttaggcgc tacaagatgt tcaggggcaa
    13621 cggcatgcag catcgacatc gcaaatttgc gggtgaaatt attaatactg atcatggtgt
    13681 taaccatttc tatctgctta tagaacttta gttttttcaa cacagagaaa atggtctgcg
    13741 gtacattttt ttctgccagt aacgccatga cttcaggggc aatgccatca gaaattttca
    13801 gtttaccaat cagcgtatct ggcgtgacac ccagtacggc gcatagtttt tcaactgaaa
    13861 cgccagattc aaccgcttta agcagcatgt tttgggcctg cacaatggta aggcgactaa
    13921 cacgtttgtt atatgaatat gtatcctcca caggggatat cagacaatga gcagtagtta
    13981 ttccgagttc tttcaaaact tcaacgcgaa gatgtccatc cagaatgact ttatcattgg
    14041 cgtcattgat ataaaccaca atgggttcaa ttaacccaat ttcatcgaca gaggctttaa
    14101 tttgtttgta cttcgtactg aatttaacat taaccggcag cgttctggca tacaccagtg
    14161 attccacctt aaaggtataa aatcgggagt caaaacattg tgaaatcatc ttatttctcc
    14221 cccgatacat cactgccatc cagtaaagaa ggaatatcgt ggatattatt actgtgcagc
    14281 aatgtaacaa aatcattatc tttaagtaaa taaccaaata tttgctttac tgccagaatt
    14341 ttctctttaa ccattgtgga tttcagcaga atggcttttt gctcattaat gttttcctgg
    14401 tagacattag ccagttcttc cggggtcagt tttttctgtt ttttcccagg aaaaaaagcc
    14461 gtactaatcg tacctttatc gcctttctta cgttgttcaa gtatgcgcct gatcttatta
    14521 atttgcccac ccttgatatg tccttctttc agggcgaccg tcagcagttc ctgagtttcc
    14581 tcatctcctg accgggatat ttccacagca agatacaagg gaatatcgcc agtctcaaat
    14641 gcccgcaaaa gatgcttctc tccggatgac aacagagtga tcaccccatt tacccaactc
    14701 gaattatacc caagccgcaa gcctatttca cgatcggtca ggccactggt cttcattctt
    14761 ctgatatctt ccattagctc tgccgcccgg ggattacgtc ttgcgatgtt ttcagccagg
    14821 ctcatcaggt gcgctgtctc ttcatcaacc tgcttgatga ttgcaggaat tttcgtttca
    14881 ccatactcaa tatacgcttc aagtcgtccc tgaccacaaa tcagggcata tttatagttt
    14941 tcattattca ctgggcgcac gctaacaggc cgtttcagac caatggtttt tatgttctct
    15001 tttatttcat tatgaatgaa tttattcctt ttccgtggat ttgcgatata aatatcactg
    15061 atatcgacaa gttctatctg ttcatcaaaa gatttcatta tttactctcc agaccagttc
    15121 gttttacaag attttccaga catgacaaat catcaaatcg atatagttcg aggataaaac
    15181 cattgttctc atgaagcgtt aaatcgttat acaacacatc aatagccgga aacataaagt
    15241 aatcaaccac ggagttattt gtgctatcca accgtggaaa gatcatgaca tcacaataat
    15301 gggatacatt aaattttatc ttccaacgaa gcttacctga cgacaaacgt tggcagcgaa
    15361 ccagattagc ggaaacaaaa aactcatcat taatatataa tgaaccatcc tgatgcatct
    15421 ctgcccaacc gccatatcgg tatatatcct gcaccaaatt atccatgaaa gtgagatggc
    15481 attctgcaat ctgctttttt atttcaagat aagagtaatc atggcgcggg tgataaccaa
    15541 taagggtgta agccctgact aacccaccaa accgtgaacg aaaaatgctg ctcgatggaa
    15601 acagttcctc ttcatcaatc acaatcccgg ataatctgcc acgaagcttc agcagatggc
    15661 gtagtttatc cagcagttca tcatcactgt actgaacggc cctggcttca ataatccttt
    15721 tagccgcctc aaatttctct actgatacca cagggtccca cgcgccgtca catctgatcc
    15781 actgatcctg aggattcctg acacttcggg tcttcaatcg tccggaggtt ctgttataaa
    15841 cattattgcc aatatatttt tcattggtca gtatctgatg aattttacat cggctccact
    15901 ggagcccatt ctcggcgggg atgttcttcc ggttcagttc tgaagcaata acaaactcag
    15961 gcttgccctc atcaataaac atgtcataga ttttattgac tattaccacc tcttccgcgg
    16021 gccccgggat caatattact ctgtcagttt gcaggctttt cctttgcccc ggcagaagca
    16081 tgcctttcgg attagtatgt tcatcaatta ataatctccg aatgccatag ccaggcatac
    16141 caccttgata ataaccccgc cgaacgaggt ttgcctggcc aagaaaaact ttttgtgaaa
    16201 ggtttttact gtaagttgcg gcgccatagc gggagtaaga taatcccagc ataaacaatt
    16261 cggtctgttc ttccgagaaa ggctccgcgc aataaataat gcgcacacca ttacgcttca
    16321 gtagccacga gtaatgagca gcctcttcaa tatcctgaaa acgaccaaac cgactgacat
    16381 cgtaaacaag aattaacgag acttttatta ttccgttttc aacatcatat aacagcgaac
    16441 gcaggccagg cctgttatcc aggctcagcc cacttcggcc agcgtcgtca taggtatatt
    16501 taatggtaat acagtgttct tttgcatact tctcgatata ttcccgctgg ttctccggcg
    16561 aatatttctg atgctcggta gacattctta aatattgagc agcaatcctg ttcccggcga
    16621 caatatccga catagaatat tccatatgat tacgcgctat ttataactca tcgtaaacgt
    16681 cgccgtaccg tttgccgcac ctggcgtgat cgagcctgca gtctggtaat atcgcgcctt
    16741 aagctgcacg atctcgtcac catataccga ctgccagtaa aaccgttcct gaccgtactg
    16801 gacagcggca tcatccgggc ggtaatgaag ttgtatcccc acgccagacg ccccgccgct
    16861 atcaatcagc atgacgcctt gcgaactggc ggaagcatcc gcagtggcat cgatacgtac
    16921 gtttattctc gccccgacat cgcagctcaa accaatatca aagctcaccc agtcggttga
    16981 agatcccgca ccactgaatt cgcttttatc gtgatccccc atcgggacat caacagaggg
    17041 cgtggtcaca gtacaagctt ctgatttcac tgtgttcatc ccgttcaacg tgacgttggc
    17101 aaaataaaac tgattcacta ccgaagcgcg cgccagcaca ccgtccgtta atgtaccgct
    17161 ggtgatatcc ccggttttga taagctcgcc cttgatgcca tcaccgggaa tggataccca
    17221 ggtgccgcca gcgttgtagg cgtagctggt ttcatagggc aatgttttac ctgtcgaggc
    17281 cgcactcaga cgtaatccta cccctggcag gttggtgttg tagacaccat tcccatagcc
    17341 actcagcgtc ccccattgcg tcagttccca gcgggcggtc caggcctccc gcttacaccc
    17401 ggcaatgccg tcgccattgt tgtatgcccc tgtaatggca gtggcaatcg tgctgccgac
    17461 ctgtgcatcc cgctggataa ccacgttgcc gaaattcagg taacccgata cttctgatgt
    17521 cacaccaccg tagaacgaac aggtggcctg aacctccggc aggacggcta tcatcaacag
    17581 caggaagaaa agaatttttc tcatctcaac actccggcga cgtcatttat aggtcagcgt
    17641 gaaggtcgcc gtggcattgg caacacctgc agtaatcgtg gctgcggtct ggtaataccg
    17701 cgccttaagc tgcactgtct cagtaccgcc attgggagag gtatagtaat agcgtgactg
    17761 accaaactgt acggcgctgt tatccggtgc aaagaacaac tggacaccga tgcccgtagc
    17821 cgccatgtct ccgggcgcgc tgtccagctt catcacgcca gccacgttgg acggatcctg
    17881 tacggcatct atctgaacgt tgatacgggc acttttatcg cagtccagcc cgatgttaaa
    17941 cgcctgccag ccggttcccg ttcccggccc ggagaactcc gctttttgat gctgccccag
    18001 cggcacattg acatcaggcg tggtcactga gcaggcgatg gtggtgattt gcccaccgga
    18061 aaatgtaatg ggaatttcag tcgaccactg cccgttctcc cttgtccccg cgataaaacg
    18121 ccccaggttg ccgctcagcg taccgggcgt aatgtcgccg gtttttatca gccggatccg
    18181 cgcctgatcg ttaatggagg gatggtagcc aacgctgtgg cttatctgga gcgtgaatac
    18241 ctgcgactgc ccgctggcga taccgatcca gttttccggt aacggattgc tgtcgaccga
    18301 ggagcggcct tcaaacacaa atcccacacc tgcgacatta ctgttaaaca ccgcctcacc
    18361 attccaggtg acgctggcgc gggagcccgt gtttttgatg cctgcattga tgacgtattg
    18421 tggcgttgca tcataattgc agtcctgata ggccatcccc tggttcgaca ccagccagtc
    18481 gctaatgggt tcccccacca cagcatcccg ctggaccacc accgtaccgg ggctgatgga
    18541 gaaactcacc gaagacgtgc aaaacgccca cgacgcctgg cagaacagga tccccgccag
    18601 cagaatcatc ccgcgccata ctctcatttt gccacctccc tgcattcctg gttcaccagg
    18661 atcactgacg gctggttttc ctgttccggt aaacggtaat gcaccacgca ctgttgtgct
    18721 ccgttccagt taaccgcaat atcgccttcc tccggcacgc cagtcagata gacctggcca
    18781 tcatcgccga caataccgct gccgccctct ttcagcttcg cctctgcgcc aaaggggact
    18841 ggcagcccac ggtacagcag cgtcatgagc acacgccgcc cgacacgggt gttaaaatcc
    18901 gccatcacca cggcaccctg cgtgggggtc acggtctgta cggcagtgtc ggtatccact
    18961 tcgtcgccaa gcgtgctggt gtcgagcgca atacggtttt tcttgtacgg gttcacatac
    19021 ggcactaccg cataaccgcg ccagtcggtg taaaccccgg tgttgttctg taccttcgta
    19081 tcatccgcac cgggcgcttt cacgatcgcc agcgactggc cgagcggctg cgaaagcgtg
    19141 atgccatgcg gatgcgcgac aataccgccc ttcagcccgt agttaacctg ctgcgtgtcg
    19201 tcgtcatagt tgtagcctcc ggtgatttcc ccctgcccgc ctttatagtc ggcgttgata
    19261 ttgccggagc cgcccacacc atggttggtg tagctctgct ggaggctgta gctcaggttg
    19321 ttgtcggcaa gcgccgtacc gttcagccct acctgctggc gggtatcgcc gtgtttcgcg
    19381 gtgttgacgc tgtagctcgc ccaggcgttc ggcataaagc ggctgagcgg cacctggatg
    19441 ttgaacgcca gttgctggtc ggcaggctgc gtgctctccg ggtagtcgct gtaactccag
    19501 gtaacgctgt agctgatacc gtcgatattc ccgttccagc ccgcgctcaa cgtgcgttcg
    19561 tagccgtcct cctgccagta atcctgctgg tagccggaga taaaaaacga gccatagcta
    19621 ccgatactct gactgagatc taactggagg cgctggcgtt tgttgtacgt catctgccag
    19681 ccgttatcat catcagccct caggtcgttg gcttcctgga aggtgtaaaa tccggcggtg
    19741 gagtagcgat atgccgccag cgtgaagctg gtgtcggtgg tctgaaaatc cttcgaatac
    19801 tgcacccggt acgactgccc gttgtgggtt tcatcgttat tgagcgtggt atgggcggcg
    19861 gtgatatcgg ctgacagcga accgagttca ccgaggccaa aaccgagccc tgcggcgcca
    19921 gactgataat cgtccgagta cagcgtgccg ccatagacgg tgatagcatg cggcagaccg
    19981 taaatcgcag tgacctggcc gaactgcggc tcgtcgctgt cctcattgcc gctgcggtat
    20041 ttcccggcgg tcagggcata tttcaggcgg ccttcacgtt gcataatcgg caccgccgag
    20101 aacggctgga cgaaggtgcg ctcgctgccg tcggcctctt tgatggtcac ttccaggtca
    20161 ccgctgcccg aggtcggata cagatcggtg atggtaaacg cacccggcgc gacgtagctc
    20221 tggtagatga tgtagccgtt ctgcttaatg gtcacctgag cgttagactg ggcgataccg
    20281 cggatcaccg gggcgaaccc gcgcaggctg tccggcagca tgttgtcgtc agatgccaac
    20341 tgcgcgccgc gaaactggac gctgtcgaag atgtccgacg gcgtgtagct gtcgccgagc
    20401 gtcagttgtc ccttcaggcg ctggatatcc cgttgcaggt acgtgtttac tgaatcccag
    20461 tggctaccat tatcttcgtc ataattccag gtcgagtagt tgcgtagccg ccacgcgccg
    20521 aggttgatac cgctttgcag gttcgcgtag tagttatcat ccgaaccgtt ttgtccatcg
    20581 cgccaggtgt tgctgccgct gaggttgtag ttcagcagtg cggcggggac accttcgtcc
    20641 cattcggacg gatccacgta tcctctggcg ctggcattca ttgccgcctg gggaatactg
    20701 acgttcaact gcaatttact gaagcgcagt tcagtgttgg ccatcgggat atagcggcca
    20761 atatcatcca gtggttgatc cggcggcagt gctgccagtg ccgggaagac gtccgttttg
    20821 acgccccagg ctttcagcat cgcaggcgtt atctgcgggc tgagtttctt tttggctccg
    20881 tcaataaaag caatatcctg tgcgtcgctc tgcttttcac cgttcacgaa gaccgttacg
    20941 cggtaggtac caggtagctg accaccggat tccgcaaagg tggataaatc cgccaccggc
    21001 tggccggggt tatcaatctc cagcgcgtgg gggttaaaca cctcacgcgc ctcggcatta
    21061 cgcaaatagc cagacaaaag cactattgcc aacaacagcg tcagcaggat tatcatcaga
    21121 cgtggcgtcg ggttattcat cacgtcctcc ttcacagctt acggctttcg gtctggctga
    21181 caccgccata gtcgctgatg gcctgccagg tcaccgcgct tgcagtcgct gacggtagcg
    21241 tgaaactcac actgcctttc ggcggcacca tatccgcctc tttaatctcc tgcccgtcga
    21301 ccttcagggt gaacagcgtg acgtaatacg gtgtgggatt gctgacggta agatggttac
    21361 cgctgtgggc aacgctcaac tggtgatagg cactctccgg ctgcccttcc aggcctgccg
    21421 gacggtaaaa cagtttcagc cgcgacttga ccactacctg caacacgttg gtgcgcgaat
    21481 ggctgtcgtc ggtagcgggg atcgatttca cgttcagcca gaacaccgac tccctgtcct
    21541 gcggcaggtt gcctccggtg cgcacaatgc gcagcatgtt ctcttcgccg ggattaatgc
    21601 gaaacaacgg tggcgtgacg ataaacggcg ttttgccccc ctgttcgcca ctgtctaccc
    21661 agctttgcac caggtagacg ccggatttct ccgggttgcg taccgaggcg gccgtctctt
    21721 ttttatcccc gtcataaatc acgcgcgtac cgccaatcac gatcccggcc tgcgcctgta
    21781 cgctcaggaa agccagcaac agtgccagca ccacgtctgc atacctgttc atcttcttgt
    21841 tctccggacg aaaaatacgc atccctgcga aacagtccct gttatgcggc ggtacaccag
    21901 gtcgggtagg atgggagagg tggtgaaccc cgcgacggca cagcgttatt tgtagttaat
    21961 ggtgaactgc gaggtaccgt tagctgtacc tgtggttacc gcggtaccgg tggaaacata
    22021 gcgggcgacg aagtccagcg tattgctgcc tgccgcgagg gggtaatcca ttgaggccgt
    22081 atgcagagga atgactgcac catttttatc ggcgatttcg ataccaacgc cggtcgcaat
    22141 cccggtaccg ctgtccagcg ccagcaggtt ctggtttacg ccatcagacg tcccgtcaaa
    22201 cttcacgttc gccccggtaa cggtatcagg gcaaccactc aggttgagcg tgaagcgcgt
    22261 cggggaggct ttcatcccgg ccgcaccgtt cagtgcggtt tgccctacat tgcccagatc
    22321 gacgttcagg ttttgcgaac cgctgtccac ggtgcatgtc tggtcggtaa tactgccggt
    22381 aaaatggatg gtgccatcat tggcgaccgc attcccggcc accagtaaaa aacacgcgct
    22441 taacatcgtt tttttcatac gtttctcctt gatgaaagat cactcagggc tcaaccctga
    22501 ttcactctgg tgacgagaaa acccgcgtaa gactttcccg ttagtgccag agtggtgagt
    22561 tcaggagaaa atgtggtgga cgaagggggt gaaaaataaa atgtcagtgg aagtgaagta
    22621 tggctttcaa aatggcataa aaccgtaatg cggtgagtga gtaaaaactg gcaacgtgaa
    22681 ccacttaagc acgttgccag ccaggaccag agagagacta catatttgtt acattaaata
    22741 ttattttaaa aacaacaaga tagtccgtta ccaaaagggg tctcagaaaa attttgacag
    22801 tttgctaaat ttttctgata ctactttaca ttctggcact aattgtgtgg ttttctctca
    22861 cgtttcccca gacattcata aacctctgcg tatttgtcgc cgtataaatc accgtatgcc
    22921 ggatgttccg gtgccccaga taatcctgta tcagacgggt gtcgcgcccc agatctgcca
    22981 gcgcgtaacc gcaaccgtgt ctcagcatat gcggataagc ggaaatgctg acacctgcca
    23041 gttctccgta acgtttgagt aacaatctaa cctgatggcg cgacagcgca ccacccttct
    23101 gcgacaggaa caaccagtct gaatccgcat cccgccagcg gttgcgtacc gccagccagc
    23161 gcatcagcgc aggcagttca ttatcaaaca gcggatgctg cgtggagaga ctgtttttca
    23221 accgccggac atacaccgag cgcccgatca gatcgatatc agacaggcgc atcccgcaca
    23281 gttcactgac ccgcaggccg tgtaaaaaac acatctgaat gaggcaataa tcgcgctcaa
    23341 aatggatccc tctgtctgct gcaagcaata tctgtcccac ttcatgacga gtgagatgct
    23401 ttcgtttttc aggcatcagc cttgctcctt atcaaaataa aaaatgaaaa aaaatcaggc
    23461 cagaactgcc ttcacagtcc aggcctgaac cagagaatac caccagaaac aacgtctggt
    23521 gatgatgtaa aactcaacaa ttttctgtaa cggcttccag tttcataccg ttgatgaaca
    23581 gatattgcgg tacacccgac agaagaaata tctgctgtcg gaggcgcgat gcgagcccaa
    23641 tacatccttc tgctgctgtg cgctgctttc cccctcaggg cgattactac gccggagatc
    23701 gcagcctctg cgcttacgcc ggactgtgtg aaataccggg tggtcggcct gtgctactgg
    23761 ctgtactgca cgccgttcgg ctgtacggta cgcacgtcgg tgaaggtcag ccatttccgc
    23821 ccggatctgg tggtgtcggc ttacagcaac accgggcaga atccgtgggc ggagatgtcg
    23881 ttactcagcc caccgttgcc cggtgttgcc gaaggcggcg gcgatacgaa tcctcgttct
    23941 gccagccagc acagcaaggt ccgctttaag aatgccgatg ccatcggctt cccggcgggt
    24001 gatgaactgg caaaattttt cacccagttt ggctatatct gctcaccttc atcgcagcct
    24061 tttcaaccgt actttctgag tcaacccgat acgctggcgt ggcgcagcgg tgtgccggaa
    24121 atgacatacc cggaggcgct gacgccgggc atgcgggaag tcggtcagaa cggcgatatg
    24181 tggggcaata tctacccgcg agccggagcc atcaaccaga cgcacgacta taaagccgcc
    24241 gccgttatcg cccagcgcgc ggccgatctg gtgacccgta ccgggcaacc gcatctctac
    24301 acgccgctga ccgcctcatc gcgcgccggg tactggccgc cctccccggt tatcgaaggc
    24361 gacagcgata accatcgctg gcaaatgctc acgccgaaaa aatccgccgc ctgttcggtg
    24421 ttcccggatg gcagtgccac tgacacctac gccaacaaac tggcggagga tggcgcgtac
    24481 gcctggacgc tgtggcggcc ctataaatgc tgtccgcgcc gggggcaaac ctttctcggc
    24541 agtacgggtt aacggaggcg gtgatgagag cccttttcct ggtcttattg tttttcgccg
    24601 gggtttgcgt ggcggcagac gactacagtt tccgcacgcg aggcgcggtt gacgacctgc
    24661 tttactacca gattggcggc ggtgaagcgg tcatgccgcc tccggtacgc cgtagcccct
    24721 cgccgcttag cttaggtctt ggctggaacg ccgatatgat gtgcggcaat ttcgacatcg
    24781 acaccaccgt gcgcaaccag ctcaacggca tcaccgacgg cttccagcaa ctgatgggca
    24841 acgttatcca gagtgccacc ggggcggtcg ccagcctgcc cgcgatgatt atccagcgcg
    24901 ccaatccgca gctttatgat ctgctgacca acggcgtgct gcaggggcgg ctggattttg
    24961 ataagtctct gctgaactgc cagaaaatgg cggagaagat gaccgatttg gcggccgatt
    25021 ccgcctggtt ttccggagcc aaagcggaga actaccagtc cctcgccgcc agcgataacg
    25081 atgccatccg caccgaccag aaagcggcaa aagaggccgc cgaaaagggt aaacgctggg
    25141 tgggcggtga aaagcgcggc ggcaaaggcc agctgccgat taaggtagtc catgacgcca
    25201 ccgccgcagg atggaatatc ctcaaccagc agcctgccac cagcactact tctcttagct
    25261 cctctgaatg cgacggggaa ctttgtagca cctggacatc ccctgaagag gccgccgggt
    25321 ggatgacccg cgtcgttggc gagcagacca tcagcgtggc gcaggtcacc gacgatccgg
    25381 atgcgcgttc cggcgcgcag gcggggatcg gcctgcatcc gctgatcgac gaagaaaagg
    25441 cgaagatcct gcccctcctg acagatctgg ttaatggcag tcagaaaccc ggcttcgaca
    25501 atttggcgaa agcttccggc ggttctatgc aactgacccg cggcgtggtc gaggcgctgc
    25561 gcgacgatcc ggacgttgcc gtcctcaccg aacggctggc gggagaactg gcgctggccc
    25621 gcgtcaccga gcaggcgttg ctggcccggc gtacgctgct cgcaggtatg cgtgaaccca
    25681 atatcgccaa cgtcaaagag gcgcaggaga gcctgggcaa aaccaccgcc caactggatg
    25741 aagagctgaa ccagttgaag ctggaactcg atttccgcca ggcactgacg cgcaataccg
    25801 ccagccagat cctgcaacgc aaacagcagc gcgaccagct tcagggacag gtggtggagg
    25861 tgccggacga cagcgacagc cgtctgcaca acctcaataa cccgcagccg gacagcccgc
    25921 gatgaccgcc aacagctatt tcgagtacgt gctcaccctg ctcggctggc tggtgaataa
    25981 cggcgtgtgg cagacgctga ccgccaccgg gctgttcgcc gtgcccctgc tggcaaaact
    26041 ggttgccctg tggctgaaag cgcgcggcca gggggccgat gaaggcaacg tcgcccgcct
    26101 gctggtcgtc tgggtggagc actcgctcta cagcgcactg ctggtgatct tcttcgcggg
    26161 tgtgccgttc ctcaatgtcg atatcaacac cctcacctac gacagccagc gcacaaagca
    26221 gtgcggctgg tcggtggtac agcccggcga ttccggctac gcacctttga ttaatgaact
    26281 cagcggccag agcgcttccg ttccagtgtg gtggtacttc acgcatgcac tgggcaaagg
    26341 catcaccagc gccgccgtcg ccgtattgcc ctgcaagccg gacctgcgcc agttgcgctt
    26401 tgaggtgcag cacacccggc tcgccgatcc ggcactggcg caggagctac aggatttcgt
    26461 taacgactgc tacgggccgt cgcgggcaag gctcaagcgg ctgagtagcg atattagcga
    26521 ggagcaaagc cgggaagtgg actgggtggg atcgtctttt tacctcgcca cgctaggcta
    26581 ttacgaccgc gatcactccc gttcgccccg cgccgcctgg ccttatgacg ccagccgcga
    26641 cgacggtctg agcgatacgg gtggcggtgg ctatcctgcg tgtaaggagt ggtggtccga
    26701 tggcgatacg gggctgaaaa accgcctgct gaagcaggtc gataccacgc tctggcagca
    26761 actgcaaaaa ctgggacagt ctaaagagga gtatgaagaa gcggtactgc gctcgctggt
    26821 cagcccgcgc aatatgcagg tatcgcaggg cggactggtg tacaacgggt ttggtgggag
    26881 tgcgggggat aacctcagcc agacgcagtt gctgagtcgt gtgggcggga tcatcggcgc
    26941 ggggctttcg agctacgccc tgtttccggc gtttgataac gtccgccagg cgctgccgat
    27001 ggtgcaggcg ttcctcgaaa tggcgctggt gatttgtatt ccagtaattc tgctgttctc
    27061 ggcatgggat cttaaaacgg tcatcacgct gagctttgtg cagttcgccc tgttctttct
    27121 caccttctgg tgggaactgg cccgctggct ggataactgg ctgatgcaga tgatgtacga
    27181 cagcgatacc cactcatact tcaacctctg gggcctgcaa aacacctccg acgatctgat
    27241 tgtaaatatc attatgggcg tgatgttcct ggtgttaccc gcattctggc tgggggcgct
    27301 gacgtgggcg ggcatcagga tcggcgcagc agtcgctggg gggatgggca gcgcggtcgg
    27361 cgatatccgc agcgctggcg aacaggtcgg gaagatgatc gtcagtaaaa cacgcattcc
    27421 ctgaagattt cgtactgacg gtgtgatgaa cagggcttgt agctgagttt atgcgcaccg
    27481 cataaactgt cgtgtcagtg acaatctcgt ggtaggtagc tggagtctga aaaaacgcaa
    27541 cgggccgtta agcccgttgt tcgttacatc accgcagttc tatcactctc gcccgctcag
    27601 cctgctttcg gtagtggtcg tatcgctccc ggaaccatgc ttgctgttca tcactcatat
    27661 tgccggtaac ggcatcgata tcaactggct ggcccagggc gtgcatacgg gcaaaactgc
    27721 gcgccagaaa atcaaaattc tttaatgcgt ttatatcttg tcggttcatt agcatatccc
    27781 tccagtcatt gtgtttactg ttcaaggtca tatgcttttc tgatttcagt atccgcctga
    27841 aaaagatcgt tttttgagcg atgcgtgcgc atcaatctac ctgtgttcac atttcaggaa
    27901 cgacaaaact cttcagcccc agtaatgatt ttcccctccc ttttatcacc cgcgcagaaa
    27961 acactaccgc cagccatgca gtaagttcgc catgtatccg gaaggtgcga acggcgcaga
    28021 tgcgcaggat acccgtacca ttccatcccg cagggaaacg ccctgcgaag ggtgttccct
    28081 gctcatttac aggagaaaca ccatgtcgct tttcgataaa aaaccgtcgt taatgccggt
    28141 ggttatcgcc cctgatgtct ggcaatccgc agtggaattt cccgccgacc cgcagcaggt
    28201 ggaagaacgg ctgggcaatc tgttgctcgt cgtactgctg accctgcgca ccgccgggac
    28261 atcccgcaag aaagtcacct ttacgatcta ctgccagccg ccgcagggcg atatgaaagt
    28321 ccctgtcgca ttaccgctat cgctcaacta tcacggccac tatgtgcagg tctcgatggc
    28381 tgaacaggca taacctctcc ccgccggagt cgcttccgga tggaggaggc tccggcttct
    28441 ttccatgagg accacacgat ggacagaagc aacgatatct accagaaagt caccgaccag
    28501 attatcgctg agctggaaaa cggcaatgtg ccgtggctcc gcccgtggcg cgatggcgag
    28561 ccgccgttcc cgatcaatgc gttatccggt cgtccgtatc acggcatcaa tgtaccgctg
    28621 ctgtggaaca gcgccgacag gcagagcttc gccagcgacc gctggctgac gttccatcag
    28681 gtcagcgcgc tcggcgggca ggtgcgccgg ggggagaaat cctctctggc ggtgttgtat
    28741 ctgccgcgca cgcaggaaga gaccgacgca ctcggccagc cgttgctgga taaagacggc
    28801 gagccgaagg tgcggcattt cgggattatt cgtgaatttt ggctgtttaa cctgtcgcag
    28861 tgcgacggcc tgccgcaggc gctgtccgag ccgtttaccc ggcccgcaac gcctgtggag
    28921 atggctgaag ccattacgat gaatagcggt gtgacgctgc gccatcgccg ccagtcgaaa
    28981 gcgtattaca aacccgcgac cgatctggtg atgatgccgc acccgcaaca gtttgaaagc
    29041 agcgaggcgt actacgccac gctgctgcat gaactgacgc acgctaccgg acacgcttca
    29101 cgccttaacc gccccggtat tgccggagcg gaacgcaccg aagcactcta cgctgctgaa
    29161 gagttagtgg aggagatggg tagcgccttc ctgtgcgccc attgcggcat tcagggcagg
    29221 ctccagcacg ccagctatat cggctactgg ctggatatcc tgcgccagga caagcgggcg
    29281 gtgatccgcg ccagcggtat ggcgcgccac gccagtgaat ggctgctcaa cgctgtacca
    29341 caaccctgtc agcttagcgc ctgacagccc acccgcacgg ggagccttcc ccgccgggaa
    29401 gttgctcccc tttttctgag gatcaatgta tgggactcga tctcttcttt ttcgaacgga
    29461 aaaaaaacgc gcccgatgaa agccagtacg ccagagtggg ttatttccgc aaggtgaatc
    29521 cgctgcttaa atgggtggat aaacacgttg ggctggtgcg caaccaagtg ctgttagaag
    29581 tcaccaaagc aaatttgcag gcgcttctga gcgacctgca gagcctgacg ccggataact
    29641 gcggcgcggt ttatccgacg attgacggct ttttctatgg ttataccgat tatggagaac
    29701 actactggaa agacgttcgc accgtcactc tctgggtgaa ggagacgctg gagacgtttg
    29761 attttgagcg agacaggctg atgttttatg cctggtggta gtattaaagt ggccgacaaa
    29821 acgtcggtca ctttgcctcg tcggccagaa atacagcgcc agatatacac atgagagcat
    29881 accgttttta ctaataaaaa tgtaaaaggc ccaaagggag acactgcaaa ggaagggcga
    29941 caagggcaaa ggggcctgcc cggaaagggg aaatatgctg aagtggctaa aaagaccatc
    30001 ccagagccag ccacctacgc cagacgctac gctgccggag ggctggtttg cgccacaatc
    30061 cgcagaacaa ttgctggcga cgccgttacg ccagtcctgt ttgcaccagt tacggcaact
    30121 gaccccgctg ccggataccc tgtttgatag ctggatcctc gcggcgattc atcgcttcgc
    30181 cgcgttggta caactgcttc ccgcctcaca aagtcatcac cattccactc ccggcgggtt
    30241 gctggatcat tcgctggagg ttgcctgcaa cgccatccga ctgcgccagg gttatctgct
    30301 accaccggaa gcgggcgcgg aagaacaggc ccggcaggcc agcgcatgga cggtcgcagt
    30361 ggtctgcgtg gcattactgc acgatacggg aaaaatcgcc gtggatatgg agattcatca
    30421 acgtgatggc acccgctggc attcgtggca gggttatctg gcacagcctt atcgctggca
    30481 gtaccagagc cacggtcgcg actatcatct gcatccggcg gctaccgcaa tgctgattaa
    30541 tcagttgata ccctcgccat tactggactg gctggccgga tacccggcgg cttttgcgtc
    30601 cctgatttac cagctcaccg gacactacga acggtctggc gcactggctg aactggtgca
    30661 gcaggccgat aaagcctctg tcgcccgata cctcggcggc gatatcaaaa ccgcgctggc
    30721 gcaaaagccc tcttccttcc cccagcaact tcttacagcc ttgcggtcgc tggtacagaa
    30781 cgagtaccgg ctcaataatc ccgaatgcgg atccgatggc tggctgacgg aggatgcgct
    30841 gtggcttatc agcaaaacca ccgccgaccg ggttcgcgcc tggctgctcc agcatggagt
    30901 ttccagcgtg ccggaaaaca ataaccgcct gtttgacgaa ctgctgacgc atcagcttat
    30961 tgttgccaac gaagataaag cgatttggcg ctgcattatc cgatcggata aaggctggtc
    31021 gcccgttgaa ccgctgacgc tactgcgcct gacgccgtct gtaatctggg ggcaggatga
    31081 tcaacgtccg gcctgtttcg atggtgatgt aacgccggta attgaagccg aacccgctcc
    31141 tgaacttagc gcaccgactc aaccgttacc ggagatagca gacgatccga ggcttggtga
    31201 caataacaat ttcctgcagt ggctgaagaa ccacactagt ccttcagcca cggtgaatac
    31261 ccggaacggc aaggtacatg tggtggaaaa ccatcttttc ttgatttcgc cggggatctt
    31321 taaaacatac gcagcagaaa ccacaggcga tagcggcgac ggctggaaac tggcgcaacg
    31381 aatgtttcag gagtcagggc tggccctgcg ctgtaatgat gacagtttta tctggatctg
    31441 cgaggttcgg gttccacaaa agaaaagtag tcagttgaaa gggtttctga tggaagatcc
    31501 gaagcaggtg ttgggggaga aagtgccggt gaataatccg tggctgaagt tgctgcgcga
    31561 atagctattt atatcaaaat actctcttcc accgagctac agcgaatggg ctttaatacc
    31621 taaagagccg catgatattc aataattgat ttcaattctt caggcgaaaa aggcggatta
    31681 cgccgatgca acatggcgtg gcaatttggg cagacgggaa taaaatcttt tttgggatcc
    31741 agagagtaat tctctttgat gtctgatagc ggaatcaggt ggtgtatatg gataaaacct
    31801 ttcgctatct cgccatacac cttcatcata tcaataccac atactttgca cagacaacca
    31861 tgccaggcta atgcggcctg tcgggcctta cggtcgcgtt catagcgatt aaccgttacc
    31921 tgcgtagctg caccttcagt aaatacgctc tgcggtggca gttcatcagg aaaaatactg
    31981 atggcgggct gctcgttacc caaatgtttc tcaacgatct ggaacccttg atcggtaagc
    32041 aacttgacgc atttcgactt gataccacca ctaaaatctt caggggaaaa ctcaacgcca
    32101 gtcatatttg aagcagcaat accgactata gctttcggtg gataactgcg cccttcaaag
    32161 accacatcat acagccggga ctcgctgaac tggtggcgta caccttcatc ataggcacgc
    32221 atagccgcaa taatgcattc cctggaaagc tggtctggca gtttcttctt catttacaac
    32281 tcatcatccc tgaaacgaca tttgctaact ctttatttta tagcatatta atttatccgg
    32341 tgataaacag cactcttcaa tttcgcgtta ccgggatagg ggcatcagtg cagcggcaac
    32401 gtaacgtagc ggcagagaaa tcgcgcctca ttctggttgt ccagacgtac ctgaaagata
    32461 tcgtgtgtat cctgcgcgga tgcatcctca cgccacgcca gcagaccccg ttggttcatg
    32521 cggataataa tctgatcggg cgtttcgccc tgattcaatg cttcgactac ctgttgtagg
    32581 ggaagcggta gcgtctgagc aggccacatc acttcaacac ttacgagctg gcggtgtgtc
    32641 cgaggtgaca tcggtggttt cctccatggc tggaatttag cggttaatta gccatgaaat
    32701 aggggcattt tttactgttt caactactgc cgagatggct acggaatacg cgctgactga
    32761 tgcaaaaata gcttctctta tgtgctctgg aaatccagat ggtacgctag tgaaaatggt
    32821 ggttccgtcc ggatagcgcc atggcctttt tgttcatatt tccacagatc accactcctg
    32881 ctcccagcca tcagaagagt ttttttaccc ctccagctta cgacctgaag cttaaggtcc
    32941 agagcggcaa gcagcttaaa gagcgtatcc aggctggtcc cctcaggatg gttctcaaac
    33001 tctgaaaccg ttgactgttt catgcccata cttgctgcag tttcacgctg ggtcaggtta
    33061 cgttgcttgc gtgcatccct gattgcctga gacaacattt tgggtgaggt tacgttcatt
    33121 ttatccccca ggatagatat tttgagcata tcccctatag gggataaaaa ttcatattct
    33181 cagcggagaa aagattaata actaaatgaa ttaaaattac ttttcatgga aattaagggg
    33241 ggacgatatt cctcgagcca gtaaatatga acattgccag atggcagaac gcgccatttt
    33301 acaagcattt tccataacag atataagact gattgagcgg ttggattaag catgacgata
    33361 tgtttaaaaa aaccagtttt atcgacataa cgccaaaata tgtttacagc atcgacatat
    33421 caacgccata tgtacatttt ttggcatttt ttaaacatat catcagcagg ctttattttt
    33481 tgctatcgaa atctacagtc cctcgtttca atacaaaaat tccagcgtgt cagctacctt
    33541 ttctgggttt ctttattggt tttagcagtg cagcaagaga tgcagatgtc acgacttctg
    33601 ttttaacttc agaatcgatt gatttttctt gtgatgtcga gaattgcttg tcgaaggcat
    33661 cgttttttat cacgtttccg gtatgcctgt ttgtgtgaga gatagcagca ggacgagaaa
    33721 cacgcgtttt atttattagc cgggaatcct gaagttggcg tccctgttca tcctcagcgg
    33781 caagtttgtt cagatccttt tcaagaccca aaatctgcat aactgacaaa taagcgccga
    33841 ttgtcacgcc cgtcccgcct gattcaagag aacgcaaggt cattggcgac atcccggctc
    33901 gctcagcaac ccgcttcgct gtgagcttac gtcgcagtcg cgccagctta agccgttcgc
    33961 caaactcagc aagcaactga ttagtaccgg gaactaaggg agccgttttt ttagccatag
    34021 tgataatatt ttatcttttc aatacctata tagccataat attagcgcat catgccgaag
    34081 gtgcggtgcg ccgtggaagg tggaacatca agtttacctg cgttctccac tatctttgat
    34141 gggaaccagc aagcctttga ccttcagttt gatgatgtcg cgactgactc tgggctggca
    34201 ttattgataa cataagcgca tcttaatcgg gcagttggcg ttacgccctg ccattgttca
    34261 caataatacc gttcatgatt catgaacaaa actctgttgt gaacatttca tctaaaacta
    34321 catcctcaac catcacttgc cataccatcc cagaaatcat tgatgatctg ctcaatccca
    34381 gcatcaccca cgtcatgttg tcgtactatc acatcgatca ggcgttcttt atcaagatgg
    34441 agtaaatcct gcaaggatga gtgttcacta attgtcgcta tatcgcttcc caacagaaaa
    34501 gcgcccatac gtatgggatc ccagttcagg cgttcccctt cgacgggcga ttcatagatg
    34561 cgttcattct cgataacacc atattcacag ataatcttaa agagatcggt cgcgtcttta
    34621 gtactcccat gcccacgatc acgccatgca aaaagtttta acagagccag tcctggcaag
    34681 gagcagaacg gaatattttc gctgttatcc agccgcacaa ctacggtatg agaaaagact
    34741 tcggcaaatc catccacagc cattatgatc tcacgttctg gtggccaggc aatttgattt
    34801 ccttcagcaa caccaccaaa aggaataata tctagctcga tccctttgac aatcatacga
    34861 tgggcattac cttggacaac ttctgcccct tcagcaagga aagcattttt aagcgcatca
    34921 aatctcggcc agctatccac aaagacggca atatcaatat cacgggttcg gcgaccaata
    34981 cttctgctgt gtatatgatg gagcagaatt tcacgagccg ttgcacctgc aataaaaaac
    35041 gggatcccct gagcggtgca aatacggttg atctgactca gtaacgtttc agtagctgaa
    35101 gagatcggat tcattaaggt gaagatattt gtcattgatt atccttgctg tttcccagtt
    35161 tctgtcatcc ccgcttgcca gcagttcagc cacgcatagc atcgcctcgg ctttgtgatt
    35221 cagtacaaag ttcccccaaa aaccggcgat cagctgtagt ttcccccccg gaacaggttt
    35281 tagtcccagt tcctttcgcc tttgtaacaa cggatgggat gtaaaaatca cgccattttc
    35341 tggtatcagg tatcctccgc tcagctttgc cgccgcaatc tcgccgctcc agtattcccc
    35401 ggacgcaagc gtcaactcat cccatgaaac aggcgcggat aaagaaagcg aatccagctt
    35461 agggcgcaaa gctgtagcat aatctttcag ccacaaagcc tctaactcgc cttcattgat
    35521 taagcgacgc cccttcttcg atttgcggaa atagcgttgc gcttcgagat aatcaaaagc
    35581 cttgctgacc attcccagag agatacctgc ctttccggcc agactgcggt aagtctcgtt
    35641 cagggtctct gggctagaca gcaaaacaaa aagaagcttc atcacccctt cagcaaaacg
    35701 actgctggcg gaaacgggtt tcttttcata gcggccttcg atccgcacat acaagcctgg
    35761 aacctggata cgggcattcc ctgccgtatc gataaagttg atctggctgt cggcgcaata
    35821 ttccgccagt gcagacgtca ggcggttaca caccagcagc acaggaaaat ccgcaggaaa
    35881 gcgaaccatt tgttcgcgaa cagccatcag tgactcttta cggtggataa attttacttc
    35941 cagagcaaat atcactgact caccatccgg ccctgttagc ttaccccagg catcgtgctt
    36001 accatgtcta ccgctatgtt catactgcaa atggaatcct tttggcagat tctctatcgc
    36061 atcagacaaa agctgttctt ccttcatttg ttcacacctc ataactgttc atgaatcatg
    36121 aacaaaacaa tgttatgaac aaataaagaa agaagcaacg taaatgatca aaaaaagacc
    36181 gccagaaatg cgatgcctta ttcaaccttc caaacgtcat tttccattaa agaaagaagg
    36241 gtaaagagta gttggacgca ttctttcagg gttgagtaac cattcctact cccccgactc
    36301 cacctcctcc tgcgccgcca tcagaaccaa ctctgacttc gaaatcagca ggtctttcgg
    36361 cctcccacct ctcaacctgc gattagccga gccaaaccag atagccagcc cccacggtgt
    36421 tttacgctcc ttaaacagtg aaagaatcaa acggacaacc ttgagaggcc gcattttctc
    36481 atccagaaca taatcagggt ataaatcctc accatcgacc ttcagcgaga atatcttccc
    36541 ggcggctttc cagcctttcg gaccagcatc aggatcggcg gaacgaaagc ccgcttttgc
    36601 ggataattca gacgccgtca gccattccga gtcagccagt attctggctt tcagctcctc
    36661 tagctgccgg tcattgcgct cctggaaact catatggtcg agtgcgcctc ccggcgactt
    36721 tttgaagcca tacggcacaa catgcctgaa ccggtttgca tccagataat ccgcccacca
    36781 ctgcatcatc tcccaacgtt cttcgagatg ctgcgccttg tggatatacg ccgcacgcac
    36841 gcgcttgcgc tcctgatggc tcatctggcg ctctaccgcg tcgcttgacc acagccccga
    36901 ctccaccagc gcgctacagg ccatagtgcg aaaaccgtgg ccacagacat ctttctgggt
    36961 gtcgtagccc atcacccgca gcgctttgtt gatggtgttt tcactcatcg gcttgcggta
    37021 attgtgatcg ccggggaaca ccagttcagt tcccggtcgg caatgctgcc gcacctcgtg
    37081 cagcagttcg atggcctgac gtgacaatgg gaccaggtgt ggggagtgca tcttcgcccc
    37141 acgcgctgaa tatttaacgc cggggatcgg ctcacgctca gcggggatgg tccacatggc
    37201 gttacgcaga tcaatttcat cccagcgggc aaagcgcagt tcgctggagc ggataaacag
    37261 cagcagtgcc agttttaccg ccagttgagt cagttgacgg cctttgtaat cgtcgattct
    37321 ttccaggaaa tcagggataa gattcagatc caacgccggg tgatgccgta ctttgggcgt
    37381 ggagaccgcg ccggttaaat ctgatgcagg gttatgatcg ataatgccgt tctggacggc
    37441 gtagcgcatc acgcaggcag tgcgctgctg aagccgggaa gcaacatcca gtttgcccgc
    37501 tttctccact tccttgatgg gcaccagcag atctttgacc ttcattttgg tgatgtcaca
    37561 gctgccgttc gtggggaata cgtaaagctc gaaataacgc agcacccgct tcttatgttc
    37621 cgccgaccag ttcacgttac tggtgaccca ttcacgtgcc acagactcaa acgtatgttc
    37681 cggcgttatc gcaaggcgtt ctgcttttcg gcgtgcactc ggatcgatgc cggaaatcag
    37741 caataattgt gcctcttcac gcatgcgtct tgcctgtgca agcgtcaaaa gcggataagg
    37801 gcctaaagcg agctttttct ctttgtttac aaagcggtac ttgatgtacc agcgttttga
    37861 accgttaggg ttaaccagca ggtaaagtcc atgcgagtca ctgagcttat acggcttgcc
    37921 acaaggcttg gcgtggacta tagcgcgatc tgttagtgcc atttgggggc ctcactatta
    37981 tcgaactacg tctaagcccc caatttggcc cccaaatcgc gtggatgtca atggatgaaa
    38041 aatgaccaac acggaaagac cacaaaataa atatggttat tgtggaaaaa taaattacct
    38101 ttgaattaca aacggatatg gacgaatttg gacggaaatg gacacaaaaa aagccaccct
    38161 taggtagcta attattgcat cacttggtgc cgaaggccgg actcgaaccg gcacgtattt
    38221 ctacggttga ttttgaatca actgcgtcta ccgatttcgc cacttcggca ctgaagaggt
    38281 atgcggaaaa cgttgtggat tatacctgtc gcacgccacc atgcaagcgc cagcttgctc
    38341 gcacctcgct aagtgctgaa attttcagca ttgcataccg cgatgtgtaa cctttgtcac
    38401 actccaggcg ccccgccctg ccatgctcta cacttcccag accacaccag agaaggataa
    38461 aaacatgtcg atgataaaaa gttatgccgc aaaagaggct ggcggcgaac tggaagttta
    38521 tgagtacgat cccggtgaac tgaagccaca agatgttgaa gttcaggtgg attactgcgg
    38581 gatctgccat tccgatctgt cgatgatcga caacgaatgg ggattttcac agtatccgct
    38641 ggttgccggg catgaggtga ttgggcgcgt ggttgcattg ggtagcgccg cgcaggataa
    38701 aggtttgcag gtcggtcagc gtgtcgggat tggctggacg gcgcgtagct gtggtcactg
    38761 cgacgcctgt attagcggta atcagatcaa ctgcgagcaa ggtgcggtgc cgacgatttt
    38821 gaatcgcggc ggttttgccg agaagttgcg tgcggactgg caatgggtaa tcccactgcc
    38881 agaaaatatc gacattgaat ctgctgggcc gctgttgtgc ggcggtatca cggtctttaa
    38941 accactgttg atgcaccata tcactgccac cagccgcgtc ggcgtgattg gtattggcgg
    39001 gctggggcat atcgctataa aacttctgca cgcaatggga tgcgaggtga cagcctttag
    39061 ttctaatccg gcgaaagagc aggaagtact ggcgatgggt gccgataaag tggtgaatag
    39121 ccgcgatccg caggcactca aagcactggc ggggcagttt gatctcatta tcaacaccgt
    39181 caacgtcagc ctcgactggc agccttattt tgaggcgctg acgtatggcg gtaatttcca
    39241 tacggtcggt gcggttctca cgccgctgtc tgttccggcc tttacgttaa ttgcgggcga
    39301 ccgcagcgtc tctggttctg ctaccggcac gccttatgag ctgcgtaagc tgatgcgctt
    39361 tgccgcccgc agcaaggttg cgccgactac cgaactgttc ccgatgtcga aaattaacga
    39421 cgctatccag catgtacgcg atggtaaagc gcgctaccgc gtggttctga aagccgactt
    39481 ctgatccctt tgccgggtgc ctgtcgcccg gcgtttaaat ctctttggaa ggcgtctcgc
    39541 aatctgctga atttagcggt gaagagtgcg cagccgttgc ctatactcca ggcaggaaac
    39601 tggaggatat ctcatgagcg aacccctgtt aattgcccgc acgtcggaca cagaactgtt
    39661 tttattgccg ggaatggcga atcgtcacgg gctgattact ggcgcaacgg ggacgggtaa
    39721 aaccgttacg ctgcaaaaac tggcagagtc tctatctgaa atcggcgtgc cggtgtttat
    39781 ggccgatgtg aaaggtgatt taacgggagt agcggaggaa ggtacttctt cggaaaaact
    39841 gctcgcaagg cttaaaaata tcggcgtaaa tgactggcaa cctcatgcta atccggtggt
    39901 ggtgtgggat atctttggcg agaaaggcca cccggtgcgg gcgacggttt cggatctggg
    39961 gccgctgttg ctggcgcgac tgttgaatct caacgatgtg caatctggcg tgctgaatat
    40021 tatcttccgc attgcggacg atcagggatt gttgctgctc gactttaaag atttgcgggc
    40081 gattacccag tacatcggcg ataacgccaa atccttccag aatcagtacg gaaatatcag
    40141 tagcgcatcg gttggtgcca tccagcgcgg attactgtcg ctggaacagc aaggcgcagc
    40201 acacttcttt ggtgagccga tgctggatat caaagactgg atgcgcaccg atgccaacgg
    40261 taaaggcgtt atcaatatcc tcagcgccga gaaactttat cagatgccga aactgtacgc
    40321 cgccagcctg ccgtggatgc tttcagagtt atatgaacaa ttgcctgaag cgggcgatct
    40381 ggagaaacca aaactggtgt ttttcttcga cgaagcacat ctgctgttta acgatgcgcc
    40441 tcaggtactg ctggataaga ttgagcaggt gataaggctt attcgctcaa aaggcgtggg
    40501 cgtctggttc gtttcgcaaa acccgtctga tattccggat aatgtgctcg ggcagctcgg
    40561 taatcgtgtt caacacgctt tgcgggcttt tacgcccaaa gatcagaaag cagtaaaagc
    40621 tgcggcgcaa accatgcggg ccaatccggc atttgatacc gaaaaggcaa tccaggaact
    40681 ggggaccggc gaggcgttaa tctcgtttct cgatgcaaaa ggaagtcctt ctgtggtgga
    40741 acgggcgatg gtgatcgcac cttgttcgcg aatggggccg gtgacggaag atgagcgtaa
    40801 tggcctgatt aatcactccc cggtgtatgg caaatatgaa gatgaggtgg accgcgagtc
    40861 cgcctatgag atgctgcaaa aaggctttca ggccagtacc gagcagcaaa ataatccccc
    40921 cgtgaaaggg aaagaggtgg cggtggatga cggtattctt ggtggattga aggatatttt
    40981 gtttggcact accggaccac gcggcgggaa gaaagatggt gtggtgcaaa caatggcgaa
    41041 aagcgccgct cgccaggtga cgaatcagat tgtacgcggg atgttgggga gtttgctggg
    41101 ggggagaaaa agataagtca gacgtgatct gtgcccctca ccctaaccct ctccccaaag
    41161 gggcgagggg accgttcgaa ctcgtatttg actttgtcaa caataataaa gggagctttc
    41221 gctcccttgt tagattacga ttttctcatt aacagccaca ggctgattaa gaagaagctg
    41281 gcgcttggca acagtgcgcc gatgatcggc gggatgccat aaaccaacgt cagcgggccg
    41341 aagatctggt ccagtacgta gaagacaaaa ccgaagctga taccggtgac cacacgcacg
    41401 cccatcggta cgctacgcag tgggccaaag atgaacgaca gcgccatcag catcatcacc
    41461 gccacggata gcggctggaa gattttgctc cacatgttga gctgataacg tccggcatcc
    41521 tgaccgctcg acttcagata cttcacgtag ttgtgcaaac cactgatgga gagtgcatcc
    41581 gggtccagcg ccaccacgcc cagtttgtct ggcgtgaggt tggttttcca ggtgccgctc
    41641 accgtctgcg aaccggtaat ctgtttcgga ttggtcaggt cagattcatc aacctgcgac
    41701 agacgccaga ctttatgttc cgggtcaaac ttcgcggtag cggcataacg tacggattgc
    41761 agacgacgat tctcgttaaa ggcataaata ctgatgccac ctaactcctc gtcaccttta
    41821 acccgctcaa tgtagacgaa gttgttgcca tctttcgccc ataagccttg ctgggtagag
    41881 agcaacgagc cgccgtacat cgcctgcgca cggtagttac gcgccatctg ctcgccctgc
    41941 ggcgcgaccc attcgccaat cgccatcgtc agcaagacca gtggaatggc ggttttcatc
    42001 accgacagcg ctacctgcat acgggtaaaa ccagaagcct gcatcaccac cagttcgctg
    42061 cgctgcgcca gcatcccaag accaagcaac gccccaagca gagccgccat cgggaagaag
    42121 atttgcacat ctttcggcac gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa
    42181 ctcccctgcc cggctttttt cagctgatcg acaaacttga taatgcccga cagcgacacc
    42241 agcatgaaca gcgtcatcat gatggtggtg aaaatagttt taccgatata gcggtcaagt
    42301 acgccaaaag gttgcatcac accgctcctt tacgcgaaaa actggcgcgc aggcggcgga
    42361 ccggcacggt gtcccaaagg ttgagaacaa tcgccaaagc cagataaatc aggttaacgg
    42421 tccacatcca cagcgtcggg tccagcttac ctttaccgcc gttcgatttc agggaggtct
    42481 ggatcaggaa gaaaagtaga tacagcagca tggctggcag catcgacagt acgcgtccct
    42541 gacgcgggtt aaccacgctc agcggtacga ccataagtgc catcataaac acggtgaata
    42601 ccaacgtgat acgccagttc agttctgcac gggcacgatc ggtgtcagtg ttccacaatg
    42661 tgcgcatgtc catctggtcg gtatcgttcg ggtcgagcgc caccgcctgg tgaccaatga
    42721 tcgcctgata atcctggaag tccgtaatgc ggaaatcacg taacagtgca gtgccttcga
    42781 agcgcgttcc ctggttgaga gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc
    42841 cggaatcggc caccaccaca gaaggacgtg cattaccttt tggtcgaatt tgcgcgagga
    42901 acacatcttt gaaatcgctg ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag
    42961 tcgcttgctg gaattgccct tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg
    43021 ctaacacttc atcctgatga cgcgatgacc acggtcccgc ccacatcacg ttaaccgccg
    43081 cgacgattgc cgtgaatacc gcaaggatca ttgccgcttt caccagaacc gctttgctca
    43141 ggccgcaggc atgcattacc gtaatttcac tttcggtata cagtttgccc agcgtcatca
    43201 gcagcccgag gaacaggctt aatggcagga taagctgcgc catttccggc acgcccaacc
    43261 cgagaaggga gagcaccaga ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc
    43321 tcactaactt ttgacagaag aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt
    43381 tgagcgtctc ccgcaccaga tatcttatga ttatcacttt aaatacgccc gtaaaaactc
    43441 gtcttttgca ggattttagc ttgtttcatg gcttaaacgt catttattct cttgagtcgt
    43501 cgaaatcgtc gctaagataa ttatactcaa cggattcacc tctcagattt tgttctgacg
    43561 tgccaatgcc gtaataacgt taagattaac acgaagtcat cgcaacagcg gacatgagtt
    43621 acgaaagctt gcaattctat ctgtagccac cgccgttgtc tttaagattc aggagcgtag
    43681 tgcatggagt ttagtgtaaa aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc
    43741 gtgggcgtct tcgaaccacg tcgcctttct ccgattgcag aacagctcga taaaatcagc
    43801 gatgggtaca tcagcgccct gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca
    43861 ctgttgctgc accatgttcc gaatgtactt tccgagcgaa ttctccttat tggttgcggc
    43921 aaagaacgtg agctggatga gcgtcagtac aagcaggtta ttcagaaaac cattaatacg
    43981 ctgaatgata ctggctcgat ggaagcggtc tgctttctga ctgaactgca cgttaaaggc
    44041 cgtaacaact actggaaagt gcgtcaggct gtcgagacag caaaagagac gctctacagt
    44101 ttcgatcagt tgaaaacgaa taagagcgaa ccgcgtcgcc cgctgcgtaa gatggtgttc
    44161 aacgtgccga cccgccgtga actgaccagc ggtgagcgcg cgatccagca cggtctggcg
    44221 attgccgccg ggataaaagc agcgaaagat ctcggtaata tgccgccgaa tatctgtaac
    44281 gccgcttatc tcgcctctca ggcgcgccag ctggctgaca gctacagcaa gaatgtcatc
    44341 acccgcgtta tcggcgaaca gcagatgaaa gagctgggaa tgcactctta tctggcggtc
    44401 ggtcagggtt cacaaaacga atcgctgatg tcggtgattg agtacagagg caacgcgtcg
    44461 gaagatgcac gcccaatcgt gctggtgggt aaaggtttaa ccttcgactc cggcggtatc
    44521 tccatcaagc cttcagaagg catggatgag atgaagtacg atatgtgcgg cgcggcggcg
    44581 gtttacggcg tgatgcgtat ggtcgcggag ctgcaactgc cgattaacgt tattggcgtg
    44641 ctggcaggct gtgaaaacat gcctggcggg cgtgcctatc gtccgggcga tgtgttaacc
    44701 accatgtccg gtcaaaccgt tgaagtgctg aacaccgatg ccgaaggccg cctggtactg
    44761 tgcgacgtgt taacttacgt tgaacgtttt gagccggaag cagtgattga cgtggcgacg
    44821 ctgaccggtg cctgcgtgat cgcgctgggt catcatatta ctggtctgat ggcgaaccat
    44881 aatccgctgg cccatgaact gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc
    44941 ttaccgctgg gtgacgagta tcaggaacaa ctggagtcca attttgccga tatggcgaac
    45001 attggcggtc gtcctggtgg ggcgattacc gcaggttgct tcctgtcacg ctttacccgt
    45061 aagtataact gggctcacct ggatattgca ggaaccgcct ggcgttctgg taaagcaaaa
    45121 ggcgcaaccg gtcgtccggt agcgttgctg gcacagttcc tgttaaaccg cgctgggttt
    45181 aacggcgaag agtaagtttg cgttttaccc tcaccctaac cctctcccta agggagaggg
    45241 aaccgatcga gcgcaataga aattcattgc tcatctttca gggtgaggaa tgcagcaata
    45301 tttaaatcca ccacaagaag ccccatatat gaaaaacgca acgttctacc ttctggacaa
    45361 tgacaccacc gtcgatggct taagcgccgt tgagcaactg gtgtgtgaaa ttgccgcaga
    45421 acgttggcgc agcggtaagc gcgtactcat cgcctgtgaa gatgaaaagc aggcttaccg
    45481 gctggatgaa gccctgtggg cgcgtccggc agaaagcttt gttccgcata atttagcggg
    45541 agaaggaccg cgcggcggtg cgccggtgga gatcgcctgg ccgcaaaagc gtagcagcag
    45601 cccgcgcgat atattgatta gtctgcgaac aagctttgca gattttgcca ccgctttcac
    45661 agaagtggta gacttcgttc cttatgaaga ttctctgaaa caactggcgc gcgaacgcta
    45721 taaagcctac cgcgtggctg gtttcaacct gaatacggca acctggaaat aatggaaaag
    45781 acatataacc cacaagatat cgaacagccg ctttacgagc actgggaaaa gcagggctac
    45841 tttaagccta atggcgatga aagccaggaa agtttctgca tcatgatccc gccgccgaac
    45901 gtcaccggca gtttgcatat gggtcacgcc ttccagcaaa ccatcatgga taccatgatc
    45961 cgctatcagc gcatgcaggg caaaaacacc ctgtggcagg tcggtactga ccacgccggg
    46021 atcgctaccc agatggtcgt tgagcgcaag attgccgcag aagaaggtaa aacccgtcac
    46081 gactacggcc gcgaagcttt catcgacaaa atctgggaat ggaaagcgga atctggcggc
    46141 accattaccc gtcagatgcg ccgtctcggc aactccgtag actgggagcg tgaacgcttc
    46201 actatggacg aaggcctgtc caatgcggtg aaagaagttt tcgttcgtct gtataaagaa
    46261 gacctgattt accgtggcaa acgcctggta aactgggacc cgaaactgcg taccgctatc
    46321 tctgacctgg aagtggaaaa ccgcgaatcg aaaggttcga tgtggcacat ccgctatccg
    46381 ctggctgacg gcgcgaaaac cgcagatggt aaagattatc tggtggttgc gactacccgt
    46441 ccggaaaccc tgctgggcga taccggcgtg gccgttaacc cggaagatcc gcgttacaaa
    46501 gatctgattg gcaaatatgt cattctgccg ctggttaacc gtcgtattcc gatcgttggc
    46561 gacgaacacg ccgacatgga aaaaggcacc ggctgcgtga agatcacccc ggcgcacgac
    46621 tttaacgact atgaagtggg taaacgtcac gccctgccga tgatcaacat cctgaccttt
    46681 gacggtgata tccgtgaaag cgcccaggtg ttcgatacca aaggtaacga atctgacgtt
    46741 tattccagcg aaatcccggc agagttccag aaactcgagc gttttgccgc acgtaaagcg
    46801 gtcgttgctg cggttgacgc actcggcctg ctggaagaaa ttaaaccgca cgacctgacc
    46861 gttccttacg gcgaccgtgg cggcgtagtt atcgaaccga tgctgaccga ccagtggtac
    46921 gtgcgtgccg atgtcctggc gaaaccggcg gttgaagcgg ttgagaacgg cgacattcag
    46981 ttcgtaccga agcagtacga aaacatgtac ttctcctgga tgcgcgatat tcaggactgg
    47041 tgtatctctc gtcagctgtg gtggggtcac cgtatcccgg catggtacga cgaagcgggt
    47101 aacgtttatg ttggccgcaa cgaagacgaa gtgcgtaaag aaaataacct gagcgcggac
    47161 gtcgcgctgc gtcaggacga agacgttctc gacacctggt tctcctccgc gctgtggacc
    47221 ttctccacgc tcggctggcc ggaaaacacc gacgcactgc gccagttcca cccaaccagc
    47281 gtgatggtat ccggcttcga catcatcttc ttctggattg cccgcatgat catgatgacc
    47341 atgcacttca tcaaagatga aaatggcaaa ccgcaggtgc cgttccacac cgtttacatg
    47401 accggcctga ttcgtgatga cgaaggccag aagatgtcca aatccaaggg taacgtgatt
    47461 gacccgctgg atatggttga cggtatttcg ctaccggaac tgctggaaaa acgtaccggc
    47521 aatatgatgc agccgcagct ggcggacaaa atccgtaagc gcaccgagaa gcagttcccg
    47581 aacggcattg agccgcacgg tactgacgcg ctgcgcttca ccctggcggc gctggcttct
    47641 accggtcgtg acatcaactg ggatatgaag cgtctggaag gctaccgtaa cttctgtaac
    47701 aagctgtgga acgccagccg ctttgtgctg atgaacacag aaggtcagga ttgcggcttc
    47761 aacggcggcg aaatgacgct atcgctggcg gaccgctgga ttctggcgga gttcaaccag
    47821 accatcaaag cgtaccgcga agcgctggac agcttccgct tcgatatcgc cgcaggcatt
    47881 ttgtatgagt tcacctggaa ccagttctgt gactggtatc tggagctgac caagccggta
    47941 atgaacggtg gaacagaagc cgaactgcgc ggtactcgcc atacgctggt gactgtactg
    48001 gaaggtctgc tgcgcctcgc gcatccgatc attccgttca tcaccgaaac catctggcag
    48061 cgtgtgaaag tactttgcgg tatcactgcc gacaccatca tgctgcagcc attcccgcag
    48121 tatgatgcat ctcaggttga tgaagccgca ctggccgaca ccgagtggct gaaacaggcg
    48181 atcgttgctg tacgtaacat ccgtgcagaa atgaacatcg cgccgggcaa accgctggag
    48241 ctgttgcttc gtggttgcag cgcggatgca gagcgtcgcg taaatgaaaa ccgtggcttc
    48301 ctgcaaacgc tggcgcgtct ggaaagcatc accgtgctgc ctgccgatga caaaggtccg
    48361 gtttccgtta ccaagatcat cgacggcgca gagctgctga tcccgatggc tggcctcatc
    48421 aacaaagaag atgagctggc gcgcctggcg aaagaagtgg cgaagatcga aggtgaaatc
    48481 agccgcatcg agaacaagct ggcaaatgaa ggctttgtcg cccgcgcacc ggaagcggtt
    48541 atcgcgaaag agcgtgagaa gctggaaggt tatgcagaag cgaaagccaa gctgattgaa
    48601 cagcaggctg ttatcgccgc actgtaatca acacagtgaa gaaaacgaag gccggagcat
    48661 gctccggcct tttttatctc ttacagaaat ggtaaagaag gcataactcc ccgagaaata
    48721 ctggctaaga agcctttatc tggttgttct tctttattga ttaaaggaag gtcatccaaa
    48781 tcgcccagca aatacgtatt ttttgcctgc cagtcaatca tccatatttt cagcagtgga
    48841 taagccagac cgcccgtaat cccggatatc accaccaacg cacacatgcg ataaagcata
    48901 ccgtggtacg ttaaagttga gcgaaaacga atacgcccat cattcagtga caggttgctc
    48961 ataaaatgat ttcgcaacga caccgttaaa taactggtgc taacagcaat cccaaaataa
    49021 tagattaact gcgcgattat cattttacgt tgcatttcca taaattgctg taaattctca
    49081 atgtcatcat ttgcatatac agaactatca tacgcattta atatttgatc gaagataata
    49141 taaccagcaa cagcgagaaa aggcaataaa gctaaaaatg cgagaatggc atattttata
    49201 caatacgtag tatctaattt caccttgaaa cgatgtatac cgaaactggt attgctccag
    49261 agaaagctca ttaccaggct atataaagta ccattaaaaa taccaatgga aacaatacca
    49321 actattgcca tcagaaccac agatataata acactacttg aactattggc aggtagcatc
    49381 tttgtcgaga taaagaaaac agtccccatc ccaatggcca ttaaaatcgg caagaaaaag
    49441 gttacccacc agaacccttt caaagagcaa ttaaaactaa atcttacgcc gttgagactg
    49501 gtcattaagg cctgataacg cagtcctttt gctgccataa atataagaaa aaccaacagt
    49561 aacaaagtca aaacacagcc aacaagcggc atatctgctg acactgtcat taagattgcg
    49621 aaatagaaaa aaacaaaaac aagacaacta acaaaaacat tcccaccggt aattccataa
    49681 gaaaatcgtt gtccgttaac ttccatatta gcataaagat aacgcttaca tttcattaat
    49741 gcccatggta aatagatacc tagcgttata atcgttaaca acacattcac cacacaaata
    49801 agaaaatatc tctcacctgt accatgaaaa acaaaagaat ggg
//
LOCUS       DAZKJR010000018        48223 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKJR010000018 DAZKJR010000000
VERSION     DAZKJR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.018"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(25..492)
                     /locus_tag="ACHOWS_001077"
     CDS             complement(25..492)
                     /locus_tag="ACHOWS_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFQ0046698.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTTSEIRKIVVKVALTALN"
     gene            complement(504..1502)
                     /gene="yiaK"
                     /locus_tag="ACHOWS_001078"
     CDS             complement(504..1502)
                     /gene="yiaK"
                     /locus_tag="ACHOWS_001078"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFQ0046699.1"
                     /translation="MKVTFEQLKAAFNRVLISRDVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1703..2551
                     /gene="yiaJ"
                     /locus_tag="ACHOWS_001079"
     CDS             1703..2551
                     /gene="yiaJ"
                     /locus_tag="ACHOWS_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFQ0046700.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            2653..3126
                     /locus_tag="ACHOWS_001080"
     CDS             2653..3126
                     /locus_tag="ACHOWS_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0046701.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(3278..4531)
                     /gene="avtA"
                     /locus_tag="ACHOWS_001081"
     CDS             complement(3278..4531)
                     /gene="avtA"
                     /locus_tag="ACHOWS_001081"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFQ0046702.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMSEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(4709..6736)
                     /gene="malS"
                     /locus_tag="ACHOWS_001082"
     CDS             complement(4709..6736)
                     /gene="malS"
                     /locus_tag="ACHOWS_001082"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFQ0046703.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALVEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAA
                     SVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            7056..7880
                     /locus_tag="ACHOWS_001083"
     CDS             7056..7880
                     /locus_tag="ACHOWS_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFQ0046704.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(7988..9166)
                     /gene="xylR"
                     /locus_tag="ACHOWS_001084"
     CDS             complement(7988..9166)
                     /gene="xylR"
                     /locus_tag="ACHOWS_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFQ0046705.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(9244..10425)
                     /gene="xylH"
                     /locus_tag="ACHOWS_001085"
     CDS             complement(9244..10425)
                     /gene="xylH"
                     /locus_tag="ACHOWS_001085"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFQ0046706.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(10403..11944)
                     /gene="xylG"
                     /locus_tag="ACHOWS_001086"
     CDS             complement(10403..11944)
                     /gene="xylG"
                     /locus_tag="ACHOWS_001086"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFQ0046707.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(12022..13014)
                     /gene="xylF"
                     /locus_tag="ACHOWS_001087"
     CDS             complement(12022..13014)
                     /gene="xylF"
                     /locus_tag="ACHOWS_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0046708.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            13380..14702
                     /gene="xylA"
                     /locus_tag="ACHOWS_001088"
     CDS             13380..14702
                     /gene="xylA"
                     /locus_tag="ACHOWS_001088"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFQ0046709.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            14774..16228
                     /gene="xylB"
                     /locus_tag="ACHOWS_001089"
     CDS             14774..16228
                     /gene="xylB"
                     /locus_tag="ACHOWS_001089"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0046710.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVIIGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWSMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            16397..16738
                     /gene="yiaB"
                     /locus_tag="ACHOWS_001090"
     CDS             16397..16738
                     /gene="yiaB"
                     /locus_tag="ACHOWS_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFQ0046711.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGILMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            16783..17220
                     /gene="yiaA"
                     /locus_tag="ACHOWS_001091"
     CDS             16783..17220
                     /gene="yiaA"
                     /locus_tag="ACHOWS_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFQ0046712.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(17262..18257)
                     /gene="wecH"
                     /locus_tag="ACHOWS_001092"
     CDS             complement(17262..18257)
                     /gene="wecH"
                     /locus_tag="ACHOWS_001092"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFQ0046713.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPHHFLRIGLCLLFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            18431..18739
                     /locus_tag="ACHOWS_001093"
     CDS             18431..18739
                     /locus_tag="ACHOWS_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFQ0046714.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            18834..19745
                     /gene="glyQ"
                     /locus_tag="ACHOWS_001094"
     CDS             18834..19745
                     /gene="glyQ"
                     /locus_tag="ACHOWS_001094"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0046715.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            19755..21824
                     /gene="glyS"
                     /locus_tag="ACHOWS_001095"
     CDS             19755..21824
                     /gene="glyS"
                     /locus_tag="ACHOWS_001095"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0046716.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNTNVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            22146..22298
                     /gene="hokA"
                     /locus_tag="ACHOWS_001096"
     CDS             22146..22298
                     /gene="hokA"
                     /locus_tag="ACHOWS_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFQ0046717.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(22486..22698)
                     /gene="cspA"
                     /locus_tag="ACHOWS_001097"
     CDS             complement(22486..22698)
                     /gene="cspA"
                     /locus_tag="ACHOWS_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFQ0046718.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(22979..23269)
                     /locus_tag="ACHOWS_001098"
     CDS             complement(22979..23269)
                     /locus_tag="ACHOWS_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFQ0046719.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            23703..24413
                     /locus_tag="ACHOWS_001099"
     CDS             23703..24413
                     /locus_tag="ACHOWS_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFQ0046720.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(24463..25437)
                     /gene="ghrB"
                     /locus_tag="ACHOWS_001100"
     CDS             complement(24463..25437)
                     /gene="ghrB"
                     /locus_tag="ACHOWS_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFQ0046721.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAILA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(25541..26200)
                     /locus_tag="ACHOWS_001101"
     CDS             complement(25541..26200)
                     /locus_tag="ACHOWS_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFQ0046722.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            26353..28686
                     /gene="bisC"
                     /locus_tag="ACHOWS_001102"
     CDS             26353..28686
                     /gene="bisC"
                     /locus_tag="ACHOWS_001102"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFQ0046723.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVMVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(28655..29095)
                     /locus_tag="ACHOWS_001103"
     CDS             complement(28655..29095)
                     /locus_tag="ACHOWS_001103"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFQ0046724.1"
                     /translation="MIREVQHSELPAILELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(29092..29655)
                     /gene="tag"
                     /locus_tag="ACHOWS_001104"
     CDS             complement(29092..29655)
                     /gene="tag"
                     /locus_tag="ACHOWS_001104"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0046725.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            29813..30511
                     /locus_tag="ACHOWS_001105"
     CDS             29813..30511
                     /locus_tag="ACHOWS_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFQ0046726.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            30740..31948
                     /locus_tag="ACHOWS_001106"
     CDS             30740..31948
                     /locus_tag="ACHOWS_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0046727.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            32272..33963
                     /gene="eptB"
                     /locus_tag="ACHOWS_001107"
     CDS             32272..33963
                     /gene="eptB"
                     /locus_tag="ACHOWS_001107"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFQ0046728.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            34055..34131
                     /locus_tag="ACHOWS_001108"
     tRNA            34055..34131
                     /locus_tag="ACHOWS_001108"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34089..34091,aa:Pro,seq:cgg)
     gene            34166..34299
                     /locus_tag="ACHOWS_001109"
     ncRNA           34166..34299
                     /ncRNA_class="other"
                     /locus_tag="ACHOWS_001109"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            35044..36651
                     /gene="dppA"
                     /locus_tag="ACHOWS_001110"
     CDS             35044..36651
                     /gene="dppA"
                     /locus_tag="ACHOWS_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFQ0046729.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(36714..36941)
                     /locus_tag="ACHOWS_001111"
     CDS             complement(36714..36941)
                     /locus_tag="ACHOWS_001111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046730.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            36959..37978
                     /gene="dppB"
                     /locus_tag="ACHOWS_001112"
     CDS             36959..37978
                     /gene="dppB"
                     /locus_tag="ACHOWS_001112"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFQ0046731.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            37988..38890
                     /gene="dppC"
                     /locus_tag="ACHOWS_001113"
     CDS             37988..38890
                     /gene="dppC"
                     /locus_tag="ACHOWS_001113"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFQ0046732.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            38901..39884
                     /gene="dppD"
                     /locus_tag="ACHOWS_001114"
     CDS             38901..39884
                     /gene="dppD"
                     /locus_tag="ACHOWS_001114"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFQ0046733.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            39881..40885
                     /gene="dppF"
                     /locus_tag="ACHOWS_001115"
     CDS             39881..40885
                     /gene="dppF"
                     /locus_tag="ACHOWS_001115"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFQ0046734.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(40915..42186)
                     /locus_tag="ACHOWS_001116"
     CDS             complement(40915..42186)
                     /locus_tag="ACHOWS_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFQ0046735.1"
                     /translation="MQHNTLPKHDQKMPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            42662..42769
                     /locus_tag="ACHOWS_001117"
     CDS             42662..42769
                     /locus_tag="ACHOWS_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFQ0046736.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
     gene            43145..43252
                     /locus_tag="ACHOWS_001118"
     CDS             43145..43252
                     /locus_tag="ACHOWS_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFQ0046737.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            43628..43735
                     /locus_tag="ACHOWS_001119"
     CDS             43628..43735
                     /locus_tag="ACHOWS_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFQ0046738.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            44111..44218
                     /locus_tag="ACHOWS_001120"
     CDS             44111..44218
                     /locus_tag="ACHOWS_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFQ0046739.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(44305..45984)
                     /gene="bcsG"
                     /locus_tag="ACHOWS_001121"
     CDS             complement(44305..45984)
                     /gene="bcsG"
                     /locus_tag="ACHOWS_001121"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFQ0046740.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDITEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(45981..46172)
                     /gene="bcsF"
                     /locus_tag="ACHOWS_001122"
     CDS             complement(45981..46172)
                     /gene="bcsF"
                     /locus_tag="ACHOWS_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFQ0046741.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(46169..47740)
                     /gene="bcsE"
                     /locus_tag="ACHOWS_001123"
     CDS             complement(46169..47740)
                     /gene="bcsE"
                     /locus_tag="ACHOWS_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFQ0046742.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            48013..48201
                     /gene="bcsR"
                     /locus_tag="ACHOWS_001124"
     CDS             48013..48201
                     /gene="bcsR"
                     /locus_tag="ACHOWS_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFQ0046743.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT        11841 a        11822 c        12526 g        12034 t
ORIGIN      
        1 gttctggcgt agacgattat tcgattaatt cagcgctgtt aatgcgactt taaccacaat
       61 ttttcgtatc tcagaggttg tttgcagaat acaacctggt cggtgaacat cctgcgggaa
      121 gaaaatagcg tagctgcccg gtatcatttc tataaatgat tcatgttcac tatcgtgata
      181 aaaaataata tcgcgctgct ctaatagtga ttcgctgact ttattatttc ccgtatcaat
      241 agcaatgccg attttctctt caccccacgc cagaaactga atatcgatat accgacgatg
      301 cacttccgga cggttttccg ccgcttcgcg tgtggttaaa tcgataattt gcgcataaat
      361 attcttgccg tcgatttcga caacgcccgg ttccagggcg ttgaagtcgg tggtgcgcag
      421 aaaatcaagc gccttttcaa tggcggcagg caaacggcac gggtcaggct gcgcgatatg
      481 tccaaaaatc atgggttatc tccttataac gcttggattt ttgcccacac gctgtcatca
      541 acggtaatac cgttacggcg gttttcggcc agcagggtag taaattcgtg gccgggtaag
      601 cgaatggcct gattttcgtc agcgcgttca gcggtggtaa cgtaatccat gatgcgttgc
      661 agtttggcat cgcgggtggg gccgtcgata agcttatcta cttcaatggc aataaaaatt
      721 tgtgaaatgc cgtattcgtc gctgttgtcc tgggtgactt cggcaaccga tgcgccgtca
      781 gaaaggagag tggcgatcat atccagcaca atcgacatgc ccgaaccttt ccagtaaccc
      841 atcggcaaaa tgcggcgatt cttctcgata acgccaggtt ctttggtcaa attgccctca
      901 tcatcaaagc caccatcgac cgggagctga cggcctgcca gacggttaac ttctaacatg
      961 ccgtaagaga acatcgacat cgacatatcg accatggtga ttggggtaga aggaatggcg
     1021 acgatcagcg ggttagtgcc gatgcgacac tcttttgcgc cccacggcgg cattacggcg
     1081 atggagttgg tccagcaaat gccaatatag cctttttccg ccgcctgcca gccgtagctg
     1141 ccgccgcgca tccagtggtt ggcattacgt agtgccacca gaccaatacc gtgatcggca
     1201 gccagttcaa tggcgcgatc catcatcttt ttcgctgtca ggttaccgat cgaacgctgg
     1261 gcgtcccact gttcaattgc gccgaggctg gttatacgtt tgggttgggc atcaggaatg
     1321 atatcgccgt tttccagttg ttgaatgaaa cgagggaaac ggttgacgcc gtgagaataa
     1381 acgccggatt cggtggtgcg ggcgaacatt tcggcacagg catcagccgt ttcgctgtca
     1441 acgtcgcgtg aaattaagac tcgattaaag gctgctttta actgctcaaa tgtcactttc
     1501 atccctggct tccttgtttt tttgatcgct tttttggctt aaaatttcaa tatgcgaaat
     1561 ctgatttcaa atatatcgat cgctttttaa caaagcaatc tgattgatga atttcaaaaa
     1621 acttaaaaat caagtggtta taaattatct gtcgagatcg tgaactacga cacactttgc
     1681 gctaccatct tgacgcgaca aaatggggaa agaagtgatg ggaaaaaaag agaacgagat
     1741 ggcgcaggaa aaagagcgtc cagccggaag ccagagtctg tttcgcgggt tgatgctgat
     1801 tgagattttg agcaactatc caaacggttg tccgttggcg catctttcgg agctggctgg
     1861 tttaaataag agtaccgtcc atcgcttatt gcagggatta caatcctgcg gctatgtgac
     1921 caccgcgccc gccgcaggga gttatcgcct gaccaccaaa tttattgccg tcgggcagaa
     1981 ggcgctgtct tcgctgaata tcattcatat cgccgctccg catcttgagg cactgaacat
     2041 cgccactggt gaaactatta acttctccag ccgcgaagac gatcacgcta ttttgattta
     2101 taagctggaa cccacaaccg ggatgctgcg aacccgtgcc tatattggcc agcatatgcc
     2161 gctctactgt tccgcaatgg gcaagatcta catggcgttt ggccacccgg actacgtgaa
     2221 gtcatactgg gaaagccatc agcatgagat ccagccgtta acccgcaata ccattaccga
     2281 gctgcccgcg atgttcgacg aactggcgca cattcgtgaa agcggagcgg cgatggacag
     2341 agaagaaaac gaactcggcg tctcctgtat tgctgttccg gtgtttgata ttcatgggcg
     2401 ggtgccgtac gccgtgtcga tttcgctttc gacatcacgt ctgaaacagg tgggggagaa
     2461 aaatctcctg aaaccactgc gtgaaaccgc gcaggctatt tctaatgaac tgggatttac
     2521 tgtcagggac gaccagggcg cgatcacata acgctttgga caagtgccaa aactttaaca
     2581 tttccttcgt tggatcaaag cagtagagac gcgctctctg gcactctgct gttttagtgc
     2641 aaaggagtga tcatgaaccg gtttattatt gcggatgcga cgaaatgtat cggttgccgt
     2701 acctgtgaag tggcttgcgc agtgtcgcat caggagaatc aggattgcgc tgcgttgtca
     2761 ccagacgagt ttatttcccg tattcgtgtc attaaagacc acacatggac cacggctgta
     2821 gcctgccatc agtgtgaaga tgcaccgtgc gcgaatgtct gccctgttga cgcgataagc
     2881 cgcgaacatg ggcatatttt cgttgaacaa tcacgttgca ttggctgtaa aagctgtatg
     2941 ctggcttgcc cgtttggtgc gatggaggtc gtttcttcgc gcaaaaaggc gagggcgatt
     3001 aagtgcgatc tgtgctggca tcgggcgacg ggaccggcct gtgttgaagc ctgcccgaca
     3061 aaggcgttgc agtgcatgga tgtcgagaaa gtgcagcgac atcggctacg gcagcagcct
     3121 gtttgaaacg tttttccggc gtttctcaac gcagggatag tgcgctttgt ttatgccgga
     3181 tgcggctaaa acgccttatc cggcctacaa attctaccaa attcaatata ttgcagaaat
     3241 catgtaggcc tgataagcgt agcgcatcag gcaattttta gtgactttca gcccaggctc
     3301 tttctatctc ttctgccaga atcttcacac ccgcctcaat tttctccggc tccggcacgt
     3361 agttcatgcg catacattga tgtgtatgtg gccacggttt atccagccct gggaagaagt
     3421 tgtgccccgg caccatcagc acgccccgtg ctttcaggcg ctggtagagc tgctcggtcg
     3481 taatgggcaa atccttaaac catagccaga ggaaaatggc tccttccggt ttatgaatca
     3541 ggcagcgatc ttccggtaaa tagcggcgaa tgatagcgat agtttcctga acacgctggt
     3601 agtaaaacgg tttgatgact gtttcagaca ggcgcagcag atcgttacgc ttaatcattt
     3661 cactcatcat cgccggacca ataccgccag gtgccaggct gataatgccg ttcatattgg
     3721 tgatggcggt gatgattttt tcattggcga tgataatgcc gcagcgggag cctggtagac
     3781 ccagcttgga aagactcatg cacagtacga tattcggatt ccacagcggg cgcgcttcgc
     3841 tgaagatgat acccgggaac gggacgccat aagcgttatc aatcaccagc ggaatgccgt
     3901 gttgattcgc cagcgcgtca agcttcagca actcttcgtc agtaatcaca ttgcctgttg
     3961 ggttcgtcgg ccgggagacg caaatcatcc cggtttcttc gccaatatgc agatgctcaa
     4021 aatcgacgtg gtatttaaac tggccttccg gcagcagctc aatattcgga cgcgcagaga
     4081 caaacagatc ttcttccagt ccggcgtcag cgtagccaat gtattccggt gccagcggaa
     4141 acagcacttt tttgatccga ccatcggcac ggcgtccggc gaacagatta aataagtaga
     4201 aaaacgcgct ctggctgccg tttgttagtg caatattctg tggttcgata tcccaaccca
     4261 acttctcgcg tagcattccg gcaagcagtg tgagtagctc cgttttcccc tgtggaccgt
     4321 cgtaattgca cagtgcatca gtcgctttgc cactttccag catgtcggtc agtagcgtct
     4381 ggaagtagtc ctgcatttcc gggatctgcg ctggattacc gccgccgagc ataatcgcgc
     4441 caggcgtgcg taaaccgtcg ttcagatctt ccatcagcag cgtaatgccg gagtggcggg
     4501 taaatttgtc accaaaaagg gagaatgtca tagcgggata tctgtcgaac tctgtagcaa
     4561 ggaaggtaac aataacgcta catttgctgg ggtgcaaatc gctctgttga agagggagcg
     4621 gtgctttatc ctgcaacgct gattaggtct gacattttat cctggttggc ggcttcccga
     4681 ggtactgacg aacgggaagc cggaaaagtt actgttgccc agcccagatg accagcactt
     4741 tatcgtcgcc atgctcacga acaaagccgt agccctgctt cagcgaaagt gtcgtttgtt
     4801 tgcccgcgcc aattgcggga tggcgggcgc ggaactggct gattttctgc cagtgcgcga
     4861 cgctggcggc agatttgccg ctaacatcct gccagttcat atccgaacgc gtgccttgca
     4921 gtggatccga accggtgggg ccgaacggac gcgaggattc atcaccataa aagatttgta
     4981 ccgcgcctgg cgctaataat aactctgctg ctttgtcgcc cccttcacgg aacaggcggg
     5041 tatcatgcga cgagaggtag ctcaacacgt tgaaatcctg caatttctcc gccatttgct
     5101 gccaggtcgt atccatctgc gccagacaat cgactgcttt agccgcctgc tcctgataat
     5161 cgaaattgat catcgcatcg aagccgtggc gatagtagtc actttgcatc acgccgtggc
     5221 cccaggcttc accggtcatc cagaacggtt tgtcatctaa cgctttatcg ggattcgctt
     5281 ttttccattc gacaagcgcg gcgctggctt cggttttcag ttgctgccag gcgggcaact
     5341 caacatgttt ggcggtgtcg acccgaaaac catcaatccc atagtcgcga acccactgac
     5401 ttaaccagtg ggttaagtaa tcgcgagggg taaagccgtc gatgacttta gcgtgggtat
     5461 ccgttttgtt tttatagaac accggcagac cagaagcggt cgttgattcg gttttgatat
     5521 ccggcaaaaa ggccagcgac atagtgagat cgtcaaatcc aggattgtcg taatcgccga
     5581 tatcggttct gatccagttt tttccccacc atttatccca gcctgttttg tcgctgaaat
     5641 taatgtaatc gttaaagcta tgccaggttt gcccggcggc aggtttccag tcgctccagc
     5701 gttcacccaa cgtttttttc acttcgtcac cagaaagata taacgcgcca aactgatact
     5761 cctgcatatc cgccagtgtg gcatagccgg tgtggttcat cacgacatca aagagaatac
     5821 gaataccgcg ctgatgcgcg ctatcaacca gtgtccgtag atcggcttca ctgcccatat
     5881 tggcatcaag attcgtccag tcctgtgtgt aataaccgtg gtaggcataa tgcgggaaat
     5941 cgccttttgt accgccgccg acccagccgt gaatttgctc aaatggggca cttatccata
     6001 aagcattgac gcccagctgc tggaggtaat ccagtttgtt ggtcaggccg cgtaaatcgc
     6061 cgccgtgaaa agtgccaatt tctgccatac cgtctttatg acgtccgtaa ctctggtcat
     6121 tactgggatc gccgttttcg aaacgatctg tcagcacaaa gtaaaccgtg gcgttatgcc
     6181 agtcgaaagg ggcaggggcg tcagtttctg cccgttccag caggagtaaa ccgttgctgg
     6241 tggcagcggg ttgtaacgtt atttgaccgt tcttcactat cgcaatttgc tggctgtaat
     6301 aatcccggac ggcggctccc tccggaaaag tggcgctgac atccagtgtg agcggtaatc
     6361 catcccattt cgggcattca cggaccaggc ttgctaccgg ttcgacggcg ttctggatgg
     6421 aaatcatcaa tgttggtgta ccggagcggg tgtctagttg cagcgtatat tcgccgtccc
     6481 tgaacaatcg ccattgaggc ggcgtgttgc tacaaggttg cagggaaagc atctgattga
     6541 gttttatcgc atccgcaggc tgccagcact gttggtcaaa atttagcgtt agtggacgcg
     6601 tacctttggg caactgcgcg tggctgacaa atgttcccgt cccctgttcg ctaaaggtgg
     6661 gaaaccccgg agaagtccag ctggcggcaa cggcgaagcc aggaaggagt gtcagaaaac
     6721 aggcggcgag tttcatggcg gatgagtcct tattgctgcg atttcagaca gtttgccagc
     6781 gataagccag acaaaactca tccctgagcg gtaagtccac aggatgagaa gcaagggtga
     6841 gcgatcgcgc acaaaaaacg gctgaatttt gcgataaacc ccacattttc tgcgatttag
     6901 cgccaatctg aatcgttaac tcgtgacata gtttcagatt tggactattc cttggggtgc
     6961 tcttgacagc tatttttgat atgatttgag attcaactct caaatttgtg aaaaatataa
     7021 ggtgttggaa tgattaaatc cgaccaggag acctgatgat tttgactccc atacgacgat
     7081 atggggcgat gattcttatg ttactcactc tggtgttttc gagtgaggtg ttagcgaaga
     7141 cgcacacaac aacagcgagt caaaagtccc acttaactaa agctagtaat aaacaggtaa
     7201 gcagtaaaca agagtattct cgcaatagtg caaagagtag ttcacttcct gatttgcgaa
     7261 aatacccttc cggaacacca aggaaaaagg cgtttctccg gactgtaatg ccttacatta
     7321 ccagccaaaa tgcggccatt actgcggaac gtaactggct gatttcaaaa cagtatcagg
     7381 gccaatggtc acctgctgag cgtgcgcgtc tgaaagacat cgccaaacgc tacaaggtga
     7441 agtggtctgg taatacgcga aaaatcccgt ggaatacctt gcttgaacgc gtagacatta
     7501 tccctaccag tatggtggcg acgatggctg cagcagaaag cggttgggga acgtcaaagc
     7561 tggcgcgcaa caacaacaat ctgttcggca tgaaatgcat gaaaggacgt tgtaccaatg
     7621 cgccgggtaa agtgaaaggg tactcacagt ttagttctgt caaagaatcg gtgagcgcct
     7681 atgtcactaa cctgaatact catccggctt actcttcgtt ccgtaaatcg cgtgcacagc
     7741 tgcgtaaagc ggatcaggaa gtgactgcca cagcgatgat tcacaagctg aagggctact
     7801 cgactaaagg gaagagttat aacaactacc tgttcgcaat gtaccaggat aaccaacggt
     7861 taatcgcggc gcatatgtaa ttgcatttcc tcgagcctta tccgacttgt cagtcggata
     7921 aggcttttgc ttgcatcagg tggttgtgct gagttacctg atgcgacgcc gacaattctc
     7981 atcatcgcta caacatgacc tcgctattta catcgcgata ctcttttggc gtcgtgtcat
     8041 atgctttttt aaaaacagag tagaaatatt gcagcgatgg ataaccgcac atttgcgata
     8101 tctcattgat cgacaaggtg gttgaaatca gcagactgcg cgctttctcc agcttctcgg
     8161 catgaatcat ggcatggatg gtttcaccca cctcttcttt aaaacgcttc tcaagattgg
     8221 agcgcgagat cccgaccgca tccagtacct gatccacttt aatcccttta caggcgtgat
     8281 tacgaatgta atgcatggcc tgaataacgg cgggatcggt cagcgagcgg taatctgttg
     8341 agcgccgttc aatgacgcga actggcggga ccaaaatccg ctgtagcggc atttcttctt
     8401 tatctaataa tcgatgcaac agttttgccg cctgatagcc catttgccgc gcgccctgag
     8461 cgaccgaaga aagggcgaca cgcgacagat agcgggtcag ttcttcgtta tcgatgccaa
     8521 tcacgcataa tttttccggt acgggaatat gtagatgttc acatacttgc agaatatgcc
     8581 gcgcccgggc gtcagtaacg gcaataatcc cggtttgcgg tggcagcgtt tgtagccagt
     8641 ctgccagccg attttgcgcg tgttgccagt tctctggcgc ggtttctaac ccctgataaa
     8701 ccactccgcg atacttttct tcggcgacaa gctgacgaaa tgcatattcg cgttcagtgg
     8761 cccaacgttt accgcttgat tccggaagac cataaaaagc aaagcggtta acgcctttct
     8821 cttttaaatg caaaaatgcg ctttcaacca gcgcatagtt atcggtggca atgtaatgaa
     8881 cgggtgggta actttcggca aggtgatacg agccaccaac cccaacaatg gggacgtcga
     8941 catcagccag cgcttgctcg atttgtttgt cgtcgaagtc ggcaatgacg ccatctccta
     9001 accagtcctt gattttatca atgcgggcgc ggaaatcttc ttcaatgaaa atatcccatt
     9061 ccgattgtga cgcctgtaaa tattccccta cgccttctac tacctgccgg tcataggctt
     9121 tattggcatt gaacagtaat gtgatgcggt gacgtttagt aaacatggtt cttttcctgc
     9181 tgaatcatgc aaaaactcaa aaccggtaat acgttaccgg ctttgagaaa atttttatca
     9241 aaatcaagaa cggcgtttgg ttgcggagtc catccataca gccagcaaca gaatcgcacc
     9301 tttaacgata tactgccaga aggtcggtac atccatcata ctcatgccgt tatccagtga
     9361 agccatgatg aatgccccca ttaccgctcc agccacgctc ccgacgccgc ctgccagact
     9421 ggtgccgcca atcacacatg ctgcaattgc gtccagctcg gcgatattcc ccgcagaagg
     9481 tgaaccagcg ccaagtcgag aactgaggat taatccggcg atggctacca ttaatccgtt
     9541 aatcgcgaac acggcaagtt tggtgcgttc aacgttaatc ccggagagac gtgccgcttc
     9601 cagattgccg ccgatggcat aaatgcgtcg tccaaatgct gtccgcgttg ccataaacat
     9661 tccgccgagt aacagcaacg tcagcagcag aacaggagtg ggaacgccac ggtaatcatt
     9721 caacagccag attgcgccta atacgatgat agcggttaaa gcctggcgac caactactgc
     9781 ggtagaggcg ggagattgca aacccaaagc ctgacggcgc attcttccgc gccattgcca
     9841 gccaacaaaa gccattaagc caagcgcgcc aatgatgaag ccagtgctgg caggtagata
     9901 gctttgccca atttgtgaca tcgcggcgct ggtgggggat acggtcgtgc cgttggtgat
     9961 gccaatgagt atgccgcgaa atgccaacat gcccgcgagg gtgacaataa atgaagggac
    10021 tttgcggtac gcgacccacc atccgttcca ggcaccgaga agcaatccca gaaccagcgt
    10081 tacaatgatg gtaagtggca aaggccagcc tagccagacg tcacaaatcg ccgcgacgcc
    10141 acctaacagc cccatcattg agccgacgga aaggtcgatt tcagcagaaa ttatgacgaa
    10201 caccattcct accgcgagga tgccggtaat cgcggtttgg cgtaacaggt tggagacgtt
    10261 acgggcgctt aagtaggcac catcggtggt ccaggtaaag aacagcatga ttgcgatgat
    10321 agctgcaatc atcacgaaga cctgcaaatt cagtgatttc agcccggaaa agccaccgga
    10381 tgtcggtacg gctaatttca cttcagacgg attgcttttc gacatgatgt tcgctcctca
    10441 atgcggcttc catcacctgc tcctgagtca ggttatgatt tatcaggttg gcttttagtt
    10501 tcccttcatg catcaccagt acacgatcgc taaggccgag cacttcaggt aattcggaag
    10561 agatgacaat aacggcaata ccctgctgga cgagttggtt aattaatttg tagatctcgt
    10621 atttcgcgcc aatatcgata cccctggtgg gttcatcaag aatgagaatg cgcgggttaa
    10681 gtaacagaca gcgagcgagg atcgcttttt gctgattgcc gccgctcaaa cgtccaatag
    10741 caaggtcggg ggacgacgtt ttaactttga gttgctggat tgattccaga atacattttt
    10801 gctctgccgc gtcatcaagc tggctaatgc caccggtaaa ttttttgagt gcggcgaggg
    10861 taatattttt accaaccgcc attaccggaa cgatgccgtc gcgctttctg tcttcgggta
    10921 ccatcgcaat cccctgggcg atggcttgct gacagttacg aatatctacc tgtttgccat
    10981 caatataaat tttcccttcc cattgtccgg gccacacacc aaacaggcac tgaatagtct
    11041 cggtacgtcc ggcaccaacg agtccggcga tacccagtat ttcgccacgt ttcagggaaa
    11101 acgagacatc attaactcgt ttaatatgac gattaaccgg atgccatgcc gtcagatgtt
    11161 caatacgtaa tatttcatct ccggtggtat gtggttcatt agggtaaagc gcggttaact
    11221 ctcgtccgac catcatggtg ataatatcgt cttcactcat tccagcagca tcacgcgtgc
    11281 caatgtgctg accgtcgcga ataacgcaaa tcgtatcgga aatcgctttg acttcgttga
    11341 gtttgtgcga aatataaata caggcgatac cgtgctgttg tagatcgcga ataatatcca
    11401 gtaaaaccga cgtttcctgc tcagttaatg aggctgtcgg ctcatcgaga attaacaaac
    11461 gcacctgttt attaagtgcc ttggcaattt caaccagttg ttgttgccca agtcctaaat
    11521 cgccaacgcg ggtatcaggt gaaatggata aactgacctg tgcgagcagc ttctgacagc
    11581 gtagcgtcat cagatcataa tccataatgc cattatgggt tatttcgtta cccaggaaga
    11641 tattttccag cacggtcaat tctttcacca gggccaattc ctggtgaatg atggcgatac
    11701 ctttgcgttc ggtatcgcgg atgtgactcg cctgaatctc ttctcccgca aaaataattt
    11761 cgccttcgta ggaaccatgg ggataaatac cacacagcac tttcatcagc gttgatttac
    11821 cagacccatt ttccccacag agtgagacga tttcgccagc attcaaccgc aagctgacgt
    11881 tatcaatcgc cttcacactg ccgaaggttt tggtaatgtt cttcatttca agtagataag
    11941 gcataacgac tccacctaag ccaattaatt cacacggcat ggagagaaat cacgcccccg
    12001 ctcctcgccg gggcgtaacg cttacagctc gctctctttg tggaatccgt ctttaactac
    12061 cgtgtctttg atgttgtttt tattcacatc gatcggtgtc agcaggcggg agggaacatc
    12121 tttcaggcca ttattcagtg tggtatctgc ttttggttcc tgaccattgc ccaactcaac
    12181 ggcaatttct gcggcagtat ttgccaacaa cgtaataggt ttatacaccg tcatagtttg
    12241 cgtaccggca gcaatacgtt taatacctgc gagatccgca tcctggccgg aaattgctac
    12301 tttccctgat aaaccttgcg cgcttaatgc ctgaattgcc ccacctgcgg tggcatcgtt
    12361 tgaggcaact acagcatcaa ttttgttatt attggcggtt agcgcgtttt ccataatttt
    12421 caatgcgttt tccggtaacc agccatcaac ccattggtca ccaacgactt taatttttcc
    12481 agaatcaacg taaggtttta acactttcat ttgtccggcg cggaacagct tagcgttgtt
    12541 atctaccggc gagccgccca tcaggaagta attaccttgc ggaacaatat cgaccagggc
    12601 ttttgcctgc agttcaccga ctttttcgtt atcgaaagaa atataaaaat cgatatccgc
    12661 atcgttaatc atacggtcgt aagctaatac tttaatacct tcttgtttgg cttcttttac
    12721 aacgttactt aatacctgac cgttataagg aataatgaca agaacatcga caccccggtt
    12781 aatcatgttt tcaatctgcg acatttgtgt ttcttcattg ccatttgcag actgtacaaa
    12841 tactttcgcg ccgagagatt ctgctttttt cacaaaaata tctcgatctt tttgccagcg
    12901 ttcaagacgg agatcatcaa tcgccatacc tattttgact tctttggcgt gtgccgcaac
    12961 gttggtaagc agaagtgagg tgcaaagggt gagtagaatg ttctttattt tcatggtgta
    13021 gggccttctg tagttagagg acagctttaa taagtaacaa tcaccgcgat aaacgtaacc
    13081 aatttttagc aactaaacag gggaaaacaa ttacagattt ttatctttcg attacgattt
    13141 ttggtttatt tcttgattta tgaccgagat cttacttttg ttgcgcaatt gtacttattg
    13201 catttttctc ttcgaggaat tacccagttt catcatccca ttttattttg cgagcgagcg
    13261 cacacttgtg aattatctca atagcagtgt gaaataacat aattgagcaa ctgaaaggga
    13321 gtgcccaata ttacgacatc atccatcacc cgcggcatta cctgattatg gagttcaata
    13381 tgcaagccta ttttgaccag ctcgatcgcg ttcgttatga aggctcaaaa tcctcaaacc
    13441 cgttagcatt ccgtcactac aatcccgacg aactggtgtt gggcaagcgt atggaagagc
    13501 acttgcgttt tgccgcctgc tactggcaca ccttctgctg gaacggggcg gatatgtttg
    13561 gtgtgggagc gtttaatcgt ccgtggcagc agcctggtga ggcactggcg ttggcgaagc
    13621 gtaaagcaga tgtcgcattt gagtttttcc acaagttaca tgtgccattt tattgcttcc
    13681 acgatgtgga tgtttcccct gagggcgcgt cgttaaaaga gtacatcaat aattttgcgc
    13741 aaatggttga tgtcctggca ggcaagcaag aagagagcgg cgtgaagctg ctgtggggaa
    13801 ctgctaactg ctttacaaac cctcgctatg gcgcgggtgc ggcgacgaac ccagatcctg
    13861 aagtcttcag ttgggcggca acgcaagttg ttacagcgat ggaagcaacc cataaattgg
    13921 gcggtgaaaa ctatgtcctg tggggtggtc gtgaaggtta cgaaacgctg ttaaataccg
    13981 acttgcgcca ggagcgtgaa cagctgggcc gctttatgca gatggtggtt gagcataaac
    14041 ataaaatcgg tttccagggc acgttgctta tcgaaccgaa accgcaagaa ccgaccaaac
    14101 atcaatatga ttacgatgct gcgacggtct atggcttcct gaaacagttt ggtctggaaa
    14161 aagagattaa actgaatatt gaagctaacc acgcgacgct ggcaggtcac tctttccatc
    14221 atgaaatagc caccgccatt gcgcttggcc tgttcggttc tgtcgacgcc aaccgtggcg
    14281 atgcacaact gggctgggac accgaccagt tcccgaacag tgtggaagag aatgcgctgg
    14341 tgatgtatga aattctcaaa gcaggcggtt tcaccactgg cggtctgaac ttcgatgcca
    14401 aagtacgtcg tcaaagtact gataaatatg atctgtttta cggtcatatc ggcgcgatgg
    14461 atacgatggc gttggcgctg aaaattgcag cacgcatgat tgaagatggc gagctggata
    14521 aacgcatcgc gcagcgttat tccggctgga atagcgaatt aggccagcaa atcctgaaag
    14581 gccaaatgtc actggcagat ttagccaaat atgctcagga acacaatttg tctccggtgc
    14641 atcagagtgg tcgccaggag caactggaaa atctggtaaa tcattatctg ttcgacaaat
    14701 aacggctaac tgtgcagtcc gttggcccgg ttatcggtag cgataccggg cattttttta
    14761 aggaacgatc gatatgtata tcgggataga tcttggcacc tcgggcgtaa aagttatttt
    14821 gctcaacgag cagggtgagg tggttgcttc gcaaacggaa aagctgaccg tttcgcgccc
    14881 gcatccactc tggtcggaac aagacccgga acagtggtgg caggcaactg accgcgcaat
    14941 gaaagctctg ggcgatcagc attctctgca ggacgttaaa gcattgggta ttgccggcca
    15001 gatgcacggt gcaaccttac tggatgccca gcaacgggta ttacgccctg ccattttgtg
    15061 gaacgacggg cgctgtgcgc aagagtgcac tttgctggaa gcgcgagttc cgcaatcacg
    15121 ggtgattatc ggtaacctga tgatgcccgg atttactgcg cctaaattgc tatgggttca
    15181 gcggcatgag ccggagatat tccgtcaaat cgacaaagta ttattaccga aagattactt
    15241 gcgtctgcgt atgacggggg agtttgccag cgatatgtct gacgcagctg gcaccatgtg
    15301 gctggatgtc gcaaagcgtg actggagtga cgacatgctg caggcttgcc acttatctcg
    15361 tgaccagatg cccgcattat acgaaggcag cgaaattact ggtgctttgc tacctgaagt
    15421 tgcgaaagcg tggagtatgg cggcggtgcc agttgtcgca ggcggtggtg ataatgcagc
    15481 tggtgcggtt ggtgtaggaa tggttgatgc taatcaggca atgttatcgc tggggacgtc
    15541 gggggtctat tttgctgtca gcgaagggtt tttaagcaag ccagaaagcg ccgtacacag
    15601 cttttgccat gcgctaccgc agcgttggca tttaatgtct gtaatgctga gtgcagcgtc
    15661 gtgtctggat tgggccgcga aattaaccgg cctgagcaat gtcccagctt taatcgctgc
    15721 agctcaacag gctgatgaaa gtgccgagcc agtttggttt ctgccttatc tttccggcga
    15781 gcgtacgcca cacaataatc cccaggcgaa gggggttttc tttggtttga ctcatcaaca
    15841 tggccccaat gaactggcgc gagcagtgct ggaaggcgtg ggttatgcgc tggcagatgg
    15901 catggatgtc gtgcatgcct gcggcattaa accgcaaagt gttacgttga ttggtggcgg
    15961 ggcgcgtagt gagtactggc gtcaaatgct ggcggatatc agcggtcagc agctcgatta
    16021 ccgtacgggg ggggatgtgg ggccagcact gggcgcagca aggctggcgc agattgcggc
    16081 gaatccagag aaatcgctca ttgagttgtt gccgcagtta ccgttagaac agtcgcatct
    16141 accagatgcg cagcgttatg ccgcttatca gccacgacga gaaacgttcc gtcgcctcta
    16201 tcagcaactt ctgccattaa tggcgtaaac gttatcctct gcctgaccgg gtgggggata
    16261 attcacatct atatatctca gtaattaatt aatatttagt atgaatttat tctgaaaatc
    16321 atttgttaat ggcatttttc agttttgtct ttcgttggtt actcgtaatg tatcgctggt
    16381 agatatggag atcgttatga aaacctcaaa gactgtggca aaactattat ttgttgtcgg
    16441 ggcgctggtt tatctggttg ggctatggat ctcatgccca ttgttaagtg gaaaaggcta
    16501 ttttcttggc atattaatga cagcaacttt tggcaactat gcatatcttc gcgcagaaaa
    16561 actcgggcaa ctggataatt tttttaccca tatctgccag ttagttgcgt taatcactat
    16621 cggtctcttg tttatcggtg ttttaaacgc acctatcaat gcttatgaaa tggtgatcta
    16681 ccctatcgcc ttttttgtct gcttgtttgg tcaaatgcgt ttgtttcgct cggcatgagc
    16741 aacataaagc tcttacacat tcaggaatga aggaatactg tgatggacaa caaaatatca
    16801 acctattctc cggcattcag tattgtgtca tggatagctc tcgttggtgg tatcgttacc
    16861 tatctgttag ggctatggaa tgcagagatg cagttaaatg aaaaaggata ttattttgcc
    16921 gtactggtat taggactgtt ttctgcggcg tcttatcaaa agaccgttcg ggacaagtat
    16981 gaaggcatac cgaccacctc catttattat atgacctgcc tgactgtctt tattatctct
    17041 gttgcgttac tgatggtagg tctgtggaat gcaacgttat tactcagcga gaaaggtttt
    17101 tatggactgg ctttcttctt aagcttgttt ggtgcagtag cggtgcagaa gaatattcgt
    17161 gatgccggaa taaacccacc aaaagaaaca caggttaccc aggaagaata cagcgaataa
    17221 ttcacataag cccggtcagt ccaatgtgac cggcctttta cttaactcac tagtctgttt
    17281 ctgtcgattc gttgtaccag catagaaagt aacaaactcg ctgccaacgt cgcgcaaaag
    17341 atccaaataa tatcgagtat tggccagttt ttaagctcaa tcccccgggt gcgcagtgca
    17401 tggataatca aggcgtggaa cccgtatatg cccaacgaat ggcgggagat taagccaagt
    17461 ccgcgaacgg tacgcgtatc aagcgtgttt ttaaccagag tcaatagcgt gattgcgcag
    17521 ataaaaacca tcggcccaca gtaaagatac caggtatcgg caaaatttcc gcgccactgc
    17581 aattcatata atgtcccgcg agagataata aaaacccccg tcgcaaacag tgctgcgcac
    17641 acccacgaca gtgctttatg ctgtgtgtcc atcatcccta tagcgcggcc caacatgcca
    17701 tacaggatgt agtaaaaagt atcgccattg atatataagt taattggcag ccattcaaaa
    17761 ccgtcaattt tctgcggcac tgtgtttggg ttagcgataa taccaatcac caccattagc
    17821 accagcaaca tttttccgcc gacgttcttc acctgaatca gcggtgaaac cagataaatc
    17881 accgcaatcg cgaagaaaaa ccataagtgg taaaacactg gcttttgcag caggtttttc
    17941 agcgctaact ccacattgat ggaggtaaac agcgcaatgt agagcagtgc gattgcgcta
    18001 taaaaaagca gacataagcc gatacgcaag aaatggtgcg gctgggcgct gcgttcgcca
    18061 aaaaagagat agccggaaat catgaaaaat agcggcacgc tgacacgaga ggcagaattc
    18121 agaacattgg cgatatccca ggtgacgggg ctaacactat gagcattggt cacataccag
    18181 gtagtggtgt gaatcatcac caccattaaa cacgctatcc ctcgcaggtt atcaatccag
    18241 taaattttgg gctgcatctg tgtctctgta tctggttaaa aaagtctgac cgataaatca
    18301 ttcggttggc gcactggaat aatctgagtt ttatcactac agcttataga ggcttaagga
    18361 aattcgtaag atatcagcca ctataccgat ataaataata agactcacct gcaaaccaga
    18421 cggtaattta atgatgatga actctttctt tccggcaatg gcgcttatgg tgcttatggc
    18481 gctagtgggt tgttctacac cgccacccgt gcagaaagca caacgggtaa aggttgatcc
    18541 tctgcgttcg ttgaatatgg aagcgttatg caaggatcag gcggcgaaac gttataacac
    18601 cggcgagcaa aaaatcgacg tcaccgcatt tgaacagttc cagggaagct acgaaatgcg
    18661 cggttatacc ttccgtaaag agcagtttgt ctgttctttt gacgcagatg gccatttttt
    18721 gcatctttcc atgcgttaag ccctgctttt tcccgtttcg tactgtatat cttccatcca
    18781 gcgggtatac tgatcccttc ctttaaatcc acacgtatcc agcacgaaat aatatgcaaa
    18841 agtttgatac caggaccttc cagggcttga tcctgacctt acaggattac tgggctcgcc
    18901 agggctgcac cattgttcaa ccattggaca tggaagtcgg cgcgggaacc tctcacccaa
    18961 tgacctgtct gcgcgcgctg gggccagaac cgatggcggc tgcttatgtt cagccttctc
    19021 gtcgcccgac cgatggtcgc tacggcgaaa accccaaccg tttacagcac tactatcagt
    19081 tccaggtggt cattaagcca tcgccggaca atattcagga gctgtacctt ggttctctga
    19141 aagagctggg catggacccg accatccacg acatccgttt cgtggaagat aactgggaaa
    19201 acccgacgct gggtgcctgg gggctgggct gggaagtgtg gctgaacggc atggaagtga
    19261 cgcagttcac ttacttccag caggttggtg gtctggagtg taaaccggtt accggcgaga
    19321 tcacctacgg tctggaacgt ctggcaatgt acattcaggg cgtagacagc gtttacgacc
    19381 tggtctggag cgacggcccg ctgggtaaaa ccacctatgg cgacgtgttc catcagaacg
    19441 aagtggagca gtccacttac aacttcgaat acgcggatgt ggacttcctg ttcacctgct
    19501 ttgagcagta cgagaaagaa gcgcagcagc tgctggcgct ggaaaatccg ctgccgctgc
    19561 cagcctacga gcgtattctg aaagccgccc acagcttcaa cctgctggat gcgcgtaaag
    19621 ccatctccgt caccgagcgt cagcgctata ttctgcgcat tcgcaccctg accaaagcag
    19681 tggcagaagc atactacgct tcccgtgaag ccctcggctt cccgatgtgc aacaaagata
    19741 agtaagaggc ggctatgtct gagaaaactt ttctggtgga aatcggcact gaagagctgc
    19801 caccaaaagc actgcgcagc ctggctgagt cctttgctgc gaactttact gcggagctgg
    19861 ataacgctgg cctcgcacac ggcaccgttc aatggtttgc tgctccgcgt cgtctggcgc
    19921 ttaaagtagc taacctggcg gaagcgcaac cggatcgtga aatcgaaaaa cgcggcccgg
    19981 cgattgccca ggcgttcgac gctgaaggca aaccgagcaa agcggcagaa ggctgggcgc
    20041 gtggttgcgg tattaccgtt gaccaggctg agcgtctgac taccgataaa ggcgaatggc
    20101 tgctgtatcg cgcccacgtg aagggcgaaa gcactgaagc actgctgccg aatatggttg
    20161 cgacttcgct ggcgaaactg ccgatcccga aactgatgcg ttggggtgca agcgacgtgc
    20221 acttcgtgcg tccggtgcac accgtgaccc tgctgctggg cgacaaagtt attccggcta
    20281 ccattctggg cattcagtcc gatcgcgtga ttcgcggcca ccgctttatg ggcgagccgg
    20341 aattcactat cgacaatgct gatcagtatc cggaaattct gcgtgagcgc gggaaagtca
    20401 tcgccgatta cgaagaacgt aaggcgaaga ttaaagccga tgccgaagaa gcggcgcgta
    20461 agattggcgg taacgctgac ttaagcgaaa gcctgttgga agaagtcgct tcgctggtgg
    20521 aatggccggt tgtgctgacc gcaaaattcg aagagaaatt cctcgcggtg ccgtctgaag
    20581 cgctggttta caccatgaaa ggtgaccaga aatacttccc ggtgtatgcg aacgacggca
    20641 aactgctgcc gaactttatc ttcgttgcca acatcgaatc gaaagatccg cagcaaatta
    20701 tctctggtaa cgagaaagtc gttcgtccac gtctggcgga tgccgagttc ttcttcaaca
    20761 ccgaccgtaa aaaacgtctg gaagataacc tgccgcgcct gcaaaccgtg ctgttccagc
    20821 aacagctggg tacgctgcgt gacaagactg accgcatcca ggcgctggct ggctggattg
    20881 ctgaacagat tggcgctgac gttaaccacg ctacccgtgc gggcctgctg tccaagtgcg
    20941 acctgatgac caacatggtc ttcgagttca ccgacaccca gggcgttatg gggatgcact
    21001 acgcgcgtca cgatggcgaa gcggaagatg ttgccgtggc gctgaacgag cagtatcagc
    21061 cgcgctttgc cggtgatgac ctgccgtcta acccggtagc ctgtgcgctg gcgattgctg
    21121 acaagatgga tactctggcg ggtatcttcg gtatcggcca gcatccgaaa ggcgacaaag
    21181 acccgtttgc gttgcgtcgt gccgcacttg gcgtgctgcg tattatcgtt gagaagaacc
    21241 tcaaccttga tctgcaaacg ctgaccgaag aagcggtgcg tctgtatggc gataagctga
    21301 ctaataccaa cgtagttgat gatgttatcg actttatgct cggtcgcttc cgcgcctggt
    21361 atcaggacga aggttacacc gttgatacca tccaggcggt actggcgcgt cgtccgactc
    21421 gtccggcaga tttcgatgcc cgtatgaagg cggtatcgca cttccgtact ctggatgcgg
    21481 cagcgtcact ggcggcggca aacaagcgtg tatctaacat tctggcgaag tctgacgaag
    21541 tgctgagcga tcgcgtgaat gcttctactc tgaaagagcc ggaagaaatt aaactggcga
    21601 tgcaagttgt ggtgctgcgc gacaagctgg agccgtactt cgctgaaggt cgttaccagg
    21661 acgcgctggt cgaactggct gaattgcgtg aaccggttga tgccttcttc gataaagtga
    21721 tggtcatggt tgatgacaaa gaattgcgaa tcaaccgtct taccatgctg gagaaactgc
    21781 gcgaattatt cctgcgcgtt gcggatattt cgctgttaca gtaataacgc cgttattaaa
    21841 aagcctgcca tctggcaggc ttttttatcg ctaaataata gagcaacctt taataatctt
    21901 ctgctgaata aagattgtct catatattaa ttttatgaga tttttttagg attatatcaa
    21961 ggagaagaaa cgaacttatt aagctagaat agctgtgggt gcttgagact gtttgtctca
    22021 ggtattcacc gaaaggcaga cagagaaaag ccccacctga ctataaatca aagtgaggct
    22081 accctatgcc tgaacaccat aaggttagcc tcttactcgt tggaaatcaa cacaggaggc
    22141 tgggaatgcc gcagaaatat agattgcttt ctttaatagt gatttgtttc acgcttttat
    22201 tttttacctg gatggtaaga gattcactgt gtgaattgca tattaaacag ggaagttatg
    22261 agctggcggc atttttagcc tgcaatttga aagagtaaga gactttggcg ggaagttatt
    22321 cccgcctttc ttacggcgtt gcgcattctc attgcaccca aatttattct tcacaaaaat
    22381 aacactagat tctattacgc gatcgattat ttatttcctg aaaaacaaat aaaaaaatcc
    22441 ccgccaaatg gcagggatct tagattctgt gcttttaagc agagattaca ggctggttac
    22501 gttaccagct gccgggcctt tagcgccgct ttcgatggtg aaggacactt tctgaccttc
    22561 gtccagagat ttgtaaccat cgttctggat agcagagaag tgtacgaaca cgtctttaga
    22621 gccatcgtca ggagtgatga agccgaagcc tttgtcagcg ttgaaccatt ttacgatacc
    22681 agtcatttta ccggacatag tgtattacct ttaataatta agtgtgcctt tcggcgatat
    22741 ggcgtgcttt acagattttg aagcgttaaa ggaatgtgca ctacgagggg tatcaacgat
    22801 aactcttgaa gggacttgcc ttactacact gctttaatgg tctgtacgtc aaaccgttga
    22861 tgtgcattaa gccacgcatt ggcgggtgat gcaacaatta tttttcatat ttatgattaa
    22921 tcggttggaa aacggtcctg tcatcaggac cgtaaacagc aataaagtgg ataaaagtct
    22981 attccatcaa ctgcttactt aatgccgggt tggcttgaat caaacgcatc aattttagtt
    23041 cggcacttga aggcttcacg cgtctggatt cccattcctt taccatggcg actgatacgc
    23101 ccaaaacccg ggcgaaatca tcgattttta accctgtccc ttttcgtaac tgctcaattt
    23161 cggtacagga cgttgttctg tgcgtcaggg taattttctg cgtttcatct ttaaaaacaa
    23221 tctgttccag gctgctcaac agctcatgca ttggatcttt atattccatt gaaaactcct
    23281 caaatcacac tgcgggatcg tgaattgcat cgaagctcat taagaatagt cgggaaaaac
    23341 agacatagcg gggtgtagtc agggcatgct ctggctatga ttcgattcag ccagtcattt
    23401 agcgtgactt atagccacct aacgatctga taaatgccga tatatctctt accactcccg
    23461 gtttgtaaag atttcgaaaa tctaacgttc tgcgaaggcg taaatctcag gctacaaaaa
    23521 aagcaacggc actatcattt tttaacaatg aaacaacaaa ttgaccttgt ttcattttct
    23581 ccatgatttt accaggaaga ttacgtagaa agggtatcct gcaagcctgt cctggactat
    23641 ccttgtcacg tcagacacgc gtgtgtcgtt gtgcgctttt tttgggtgaa aggagtaaga
    23701 aaatggcgac aggaaagtcc tgctctcgct ggtttgcgcc tcttgcggcg ttattaatgg
    23761 tagttagcct gagtgggtgt tttgataaag aaggcgatca gcgtaaagcg tttatcgact
    23821 tcctgcagaa tacagtgatg cgtagcggtg aacgtctacc aaccctgact gccgatcaga
    23881 aaaaacagtt tggtcctttt gtctctgatt acgcgattct gtatggttat tctcagcagg
    23941 tgaatcaggc gatggattcc ggtctgcgcc cggttgtaga cagcgttaac gcaattcgcg
    24001 taccgcagga ttacgttacg caaagcggcc cactgcgtga aatgaacggt tctctgggcg
    24061 tactggcgca acagctgcaa aatgcgaagt tgcaggccga tgctgcgcac tctgcgctaa
    24121 aacagagcga tgacctgaaa ccggtcttcg atcaggcatt caccaaagtg gtaacgacgc
    24181 cagctgatgc gttgcaaccg ttgattccgg cggcacaaac tttcacgcaa caactggtga
    24241 tggttggaga ctacattgct cagcaaggta ctcaggtgag ctttgtcgca aatggcatcc
    24301 agttcccgac ttcgcagcag gcaagtgaat ataacaaact gattgcgcca ttaccggcac
    24361 aacatcaggc gtttaatcag gcctggacta cagcagttac tgcaacccaa taaagagtaa
    24421 aagcccgagc gattatcggg ctttactcca cgcagtcgcg gcttagtccg cgacgtgcgg
    24481 attcacacag ttcttctcaa cctttccttg taacgcatca atcaagttat ccacggcaca
    24541 ggcggccatg ccataacgcg tctcatgagt ggcagatcca atatgcggta ctgcgacgac
    24601 gttggccatt gagagcaacg gcgaatctac tgacagtggc tcttgttcga agacatccag
    24661 cccggcagcg tgaatttccc ctttctgcaa tgcagcaatc agtgcatttt catcaaccac
    24721 cgggccacgt ccggcattaa tgaaaatggc ggaggatttc attttggcga attgttctgc
    24781 gccaaacaga tgatgcgttt catcagttaa cggcaggatc aggcaaacga aatctgactc
    24841 ttgtaacagt gtatccaaat cgcagtagcg ggcgttgaag cgttcttctg cttctttatg
    24901 gtggcggcgc gcgttataga ggatgggcat gttgaagcca aagtgcgcac gttgtgccag
    24961 cgccatgccg atccgtccca tcccgacaat gcccagtgtt ttatggtgaa cgtcagtgcc
    25021 gtaccagtcc gggcctatgc tcgcggtcca ttcgcctgct tttactcgtt ctgctacctc
    25081 cacaacccga cgagcggtag acaataccag cgccatcagc gtatcggcga cggtttctgt
    25141 taatacggtt ggcgtgtgca tcagcagaat ttttcgggcg gtaagcgcat cgacatcaaa
    25201 attgtcatag cctacggaga tcgttgatgt ggcacgcagt ttcggcattt tttccagcaa
    25261 tgcggcatca acattctcgt ttgaacccag taaaccttca gcttcggcaa gaattgctgc
    25321 attttgttcg atggtttgtg ggctgaggtt tgccacctgg tgaacggtga aatgctcttg
    25381 caggcgttgc agtaaatcat caggtaaggc tttgtagagg ataacggacg gcttcattct
    25441 tctctccatt cactgataag gcgatcacct tagcctgaaa tatgatgaag gaaaactgaa
    25501 gaatgccagg tgatttctgc atcacctggc atgaaaggga ttacagcggg cttaaggtaa
    25561 tttctacacg gcggttttgc gccttacctt ctgcagtgct gttgctggcg attgggttag
    25621 ccgggccaag gccctgagta cggatgcggc tggcgtccac gccctgggtg atcaacgcgc
    25681 tggcaacgga atccgcacgc tgctgggaga gacgcatgtt caggtcgtgc ccacccgtgc
    25741 tgtcggtata accaatcacg ttaaccgccg ttttcggata ctctttcagt accattgcca
    25801 cgccggtcag ggtgttagcg cccgccggtt tcagggttgc gctgctgctg tcgaaggtca
    25861 cattgttcgg catattgagg ataatgttat ccccgctgcg ggttacgcta acaccagtgc
    25921 cgcgcatttt gtcacgcagc ttcgcttcct gcacatccat gtaataaccc acgccgccgc
    25981 ccagagctgc gcctgctgct gcgccaatga gcgcgccttt accgcgatct ttcttcgaag
    26041 aagagagcgc accaataccc gcgccaacga gagagcccag acctgcgccg atagcagatt
    26101 tacctgcttc gcgttcgccg gtgtaagggt tagttgtaca gccagatacc gccagagcgc
    26161 cactcactac ggcggcaata agataaacac gtttcttcat tgttaatcct taataacctt
    26221 tttattcttt gccacgggtt ctgtggcggg agattatgcc gcgtgaacat gaagattatt
    26281 cctgggaata ctcggaaatt tgtaagtaat atttaactgc tcaatacatc taacctttca
    26341 ggagtcttcg gtttggccaa ctcatcctca cgatattccg ttctgactgc cgcccactgg
    26401 gggcctatgc tggttgaaac cgacggcgaa accgtgttta gctcgcgtgg cgcgttagcc
    26461 acaggaatgg aaaactcctt gcagagcgcg gttcgcgacc aggttcacag caatacgcgg
    26521 gtgcgatttc caatggtgcg caaaggcttt cttgcgtcac cggaaaatcc gcaaggcatt
    26581 cgtgggcagg atgaatttgt tcgcgtgagt tgggatgagg cgctggagct tattcaccat
    26641 caacataaac gcattcgtga ggcttatggt ccggcatcga tttttgctgg ttcctacggc
    26701 tggcgttcaa acggcgtgct gcataaggcc tcgacattat tacaacgcta tatggcgctg
    26761 gcaggcggtt atactgggca tctgggggat tattcgaccg gcgcggcaca ggcgatcatg
    26821 ccgtatgtcg tgggtggcag cgaagtttat caacagcaaa ccagttggcc gctggtgctg
    26881 gaacatagcg atgtcatggt gctgtggagt gctaacccac tcaatacgct gaaaattgcg
    26941 tggaatgcat ccgatgagca ggggctttct tacttttccg cactgcgtga cagcgggaaa
    27001 aagctgatct gcattgatcc aatgcgatcg gaaaccatcg atttctttgg cgataaaatg
    27061 gagtgggtgg caccacacat gggcaccgat gttgcgttga tgctggggat cgcccatacg
    27121 ctggtggaaa atggttggca cgacgaagcg tttctggcgc gttgcaccac aggttatgcc
    27181 gtcttcgcct cttatttgct gggcgagagt gatggtatag cgaaaaacgc cgaatgggcg
    27241 gcagagattt gtggtgttgg cgcagcgaaa atccgcgagc tggcggctat tttccaccaa
    27301 aatacaacca tgctgatggc aggctgggga atgcagcgcc aacagtttgg tgagcaaaaa
    27361 cactggatgc tcgtcacgct ggcggcaatg ttggggcaaa tcggcacacc cggcggcggt
    27421 tttggtcttt cttaccattt tgccaatggt ggtaacccca cgcgccgcgc tgcggtgctc
    27481 tcttccatgc agggcagttt gcctggcggc accgatgcgg tggataaaat ccctgttgcc
    27541 cgcattgttg aagcactgga aaaccccggt ggcgcatatc aacataacgg tatggaccga
    27601 catttcccgg atattcgttt tatctggtgg gcgggtggtg ccaactttac tcatcatcag
    27661 gataccaatc gcctgatccg tgcctggcaa aaaccggagc tggtggtgat ctctgaatgc
    27721 ttctggacgg cggcggcgaa acacgcggat atcgttctgc ctgcgaccac ctcttttgag
    27781 cgtaatgatc tcaccatgac cggcgattac agtaatcagc atctggtgcc gatgaagcaa
    27841 gtggtgccgc cacgctatga agcgcgtaat gattttgatg tttttgccga gttaagtgaa
    27901 cgttgggaga agggcggcta tgcgcggttt acggaaggaa aaagtgagct gcaatggctg
    27961 gaaacgtttt ataacgttgc ccggcaacgc ggggcaagcc agcaggttga attgccgcca
    28021 tttgctgagt tctggcaagc caaccagtta attgagatgc cggaaaaccc ggacagcgag
    28081 cggtttattc gcttcgccga ttttcgccgc gatccgcagg cgcatccatt aaaaaccgcc
    28141 agcggcaaga ttgaaatctt ctcgcagcgt attgccgatt acgcttatcc ggattgccct
    28201 gggcatccaa tgtggctgga gccggacgaa tggcagggca atgccgaacc ggaacagttg
    28261 caggtacttt ctgctcatcc ggcacatcgt ctgcacagcc agctgaatta cagttctctg
    28321 cgcgaattgt acgcggtggc aaaccgtgag cctgtcacca ttcatcctga cgatgctcag
    28381 gcgcgcggca tacaagatgg cgatactgtt cggctgtgga acgcacgcgg gcaaattctt
    28441 gccggggcag tcattagcga gggaattaaa cctggcgtga tttgcattca cgaaggggca
    28501 tggccggatc tggatttaac cgctgacggt atttgtaaaa acggcgcagt gaacgtgctg
    28561 accaaagatc tccccagctc gcggctgggg aatggctgtg cgggtaatac ggcgctggca
    28621 tggctggaaa aatacaacgg tccggaactg acacttacag cgtttgaacc accggccagc
    28681 tcataatcca ggtgggtagt tgggtttcat cctgccatgc gcagtcgaca atgtgaaaac
    28741 cctgcgcctg gtaaaaatct atcgccggtt gatttttttg ataaacctcc agcatcagat
    28801 ggggatggcg ctgctgcaca tactgcatta gcgccttacc aataccgcgt ctgacggcct
    28861 tcggtgcgac aaacattgca gccagaaatc ggccttccat aatgctgaca aaaccgagaa
    28921 gcttaccgtc ttcttcccag acccagtttt gcgcgttggc aagatacgca tcgcgcacca
    28981 gcggaatgca gtcacgccag taactcgatt ttataaaggg atgcccccag gttgtacttt
    29041 ccagccacag ttcgaggatc gcggggagtt ctgaatgttg cacttcccga atcatggttt
    29101 atttcccgga tggcaacagc agccaaccac atgatcattc accagcccac atgcctgcat
    29161 aaaggagtaa cagattgtgg taccgacaaa cttaaaacca cgttttttta gtgccttaga
    29221 tagggcgtcg gaggcgggtg tagatgtggg gatttcgctc aacgttgtgg cttgtgtcac
    29281 ctgtggttga tgattgacaa acgaccagac aaagtcggca aacggttcgc cgttctgttc
    29341 catttgcagg tacgcccgcg cattaccaat aattgcctga attttcccgc gatggcggat
    29401 aatcccggcg tcctgtacca gtctttcgac atcctcttcc tgcattgcag ccaccttcac
    29461 cggatcgaac tgatgaaagc tggcgcgata gttttcgcgt tttttgagga cggtgatcca
    29521 cgataatcca gcctgctgcc cttcaaggca gatcatttcg aacagttttt tactgtcagt
    29581 ttcaggcacg ccccactcat tatcatggta ggcaatataa agcgggtcct gactcaccca
    29641 gccgcaacgt tccatacttt ccctcgctat gtacccgatc gtaaaaattt cagccgcttt
    29701 tcttgacgca tcggtgaaaa agtcaatatt taatacaggg ctactatgcc cgatatcctt
    29761 catacaatga caataatatt gccgaattcg gctcttccct ggagtcgtgt taatgatcat
    29821 aaaaaaaagc ggtggtcgct ggcagctaag cctgctggcg agtgtggtaa tcagtgcctt
    29881 ttttctcaac acagcttacg cctggcaaca agaatatatc gttgatacgc aacccggaca
    29941 ttccacagag cgttacacct gggatagcga tcatcaacct gattacaacg atattttgtc
    30001 gcaacgtatt caaagtagcc aaagggcgct gggactggaa gtcaatctgg cggaagaaac
    30061 tcctgtggat gtgaccagca gtatgagtat gggctggaat tttcctttat atgagcaggt
    30121 tacaaccggc ccggtcgcgg cattacatta cgatggcaca accacctcga tgtataacga
    30181 gtttggcgac agtactacca cgctggccga tccgttatgg catgccagcg tgagtacctt
    30241 aggctggcgt gttgactccc ggcttggcga tctccgaccc tgggcgcaaa tcagctataa
    30301 ccagcaattt ggcgagaata tctggaaggc gcaatcaggc ctgagccgga tgacggcgac
    30361 aaaccagaac ggcaactggc tggatgtcac cgtaggcgct gatatgttgc tcaatcaaaa
    30421 tattgctgcc tatgctgcgc tatctcaggc agaaaatacc actaacaata gcgactatct
    30481 gtatacgatg ggggttagcg ccagatttta acgttataga tacaattgaa accaataaat
    30541 aacaactaat gtggcgatag tgggtgataa cttactaaat aataaatttg gtgaataatt
    30601 gtcgagtcat tcattcctga actaatgcat ttcattccgt tctgatggca tttcatgccg
    30661 tttttcccca ggcataaagt gcacttcgtt atggttgtca gcagagattt ttccttttta
    30721 ttactgcagg aatactgcca tgacaccttc aaattatcag cgtacccgct ggttgacact
    30781 catcggtact atcattaccc agtttgccct tgggtcggtt tatacctgga gcctgtttaa
    30841 cggcgcgctt tctgccaagc tggatgcgcc ggtaagccag gtcgctttct ctttcggctt
    30901 gttaagtctg gggctggcaa tttcatcttc tgttgcgggg aaattacagg aacgttttgg
    30961 cgttaaacgc gtcactatgg cttccggcat tttgctggga ttaggcttct tcctgacagc
    31021 gcattctgac aacctgatga tgctgtggtt aagcgccggt gtgctggtcg gtctggcgga
    31081 tggcgcgggt tacctgctga cgctctctaa ctgcgtgaag tggttcccgg agcgtaaagg
    31141 tctgatctcc gcgttcgcta tcggttctta tggtctgggt agcctgggtt tcaaatttat
    31201 cgacacgcag ctgctggaaa cggtgggtct ggaaaaaacc tttgtgattt ggggagcgat
    31261 tgcgctggtg atgattgtct ttggcgcaac attaatgaaa gacgcaccaa aacaggaagt
    31321 gaaaaccagc aatggtgtgg tggagaagga ctacaccctg gcagagtcga tgcgtaaacc
    31381 gcagtactgg atgttagcgg ttatgttcct gactgcgtgc atgagtggtc tgtatgtgat
    31441 tggtgtagcg aaagatatcg ctcaaagtct ggcgcatctt gatgcaattt ccgcagccaa
    31501 tgctgtgacg gttatttcca tcgccaacct ttctggtcgt ctggtgctgg gcattctgtc
    31561 tgacaaaatc gcccgtatcc gtgttatcac cattggtcag gtgatatcgc tggtgggtat
    31621 ggcggccctg ctgtttgcac cattgaatgc agtgacgttc tttgcagcga ttgcctgcgt
    31681 ggcatttaac tttggcggca ctatcacggt gttcccgtca ctggtcagtg agttcttcgg
    31741 cctcaataac ctggcgaaaa actacggtgt gatttatctc ggtttcggta tcggcagcat
    31801 ttgtgggtcg attatcgcct cactgtttgg cggcttctat gtgaccttct acgtgatttt
    31861 cgcactgctg attctgtcac tggcgctctc tacgacgatt cgtcagccag agcagaaaat
    31921 gttgcgtgag gcgcatggct ccctttaatc atctgccgcg agctactggg ctttagcctt
    31981 agtgtgaatc aaacgcttca acgcaatgta taaacaatga cgcgtaaaat ttgggccagt
    32041 taactggccc tttttactgc tttttgtaac gctaaagtct cttttcaaac ttgcattttt
    32101 gtaaatttgt gcttcatgca cactctttcc ccacactttt tccctttgct gtggtctact
    32161 tattcgcgcg tgtagatttt acttatctga ctacctccgc acttttcccc tgccgggcct
    32221 gaaaagccgc taagcagggt gttatcacct gtttgtccag ggtttgtttg catgagatac
    32281 atcaaatcga ttacacagca gaagctgagc tttttgcttg caatctatat tggccttttt
    32341 atgaatggcg cggtttttta ccgccgcttc ggcagctatg cgcacgattt taccgtctgg
    32401 aaaggcattt ctgctgttgt tgaactggcc gccaccgtac tggtgacctt ctttttacta
    32461 cgtcttcttt cgctgtttgg ccgccgtagc tggcgtattc tggcgtcgct ggtggtgctc
    32521 ttttccgcag gtgccagcta ttacatgacc ttccttaatg tggtcattgg ttatggcatc
    32581 atcgcttccg tcatgaccac cgatatcgac ctgtcaaaag aagttgttgg tctgaacttt
    32641 attctctggt taatcgccgt tagcgcgttg cctcttatcc ttatctggaa taaccgctgt
    32701 cgttacacct tgctccgaca actgcgaacc ccagggcagc gtattcgcag cctggcggtc
    32761 gtcgtactgg caggtattat ggtttgggca ccgattcgtt tgctggatat ccagcagaag
    32821 aaagtggaga gggcgacagg tgttgatttg ccgagttatg gcggcgtcgt cgcgaactct
    32881 tatctgccat caaactggct ttcggcatta ggtctttatg cctgggcgcg ggtcgatgaa
    32941 tcttccgata acaattctct ccttaaccct gcgaagaagt ttacgtacca ggctccacaa
    33001 aacgttgatg atacatatgt cgttttcatt attggtgaaa ccacgcgctg ggatcatatg
    33061 ggcattttcg gctatgaacg caataccaca ccgaagctgg cccaggaaaa aaatctggcg
    33121 gcgttccgtg gttactcctg tgataccgca accaaactct ctctgcgttg catgtttgta
    33181 cgtcaggggg gcgcggaaga taatccgcag cgcacattaa aagaacagaa cattttcgcg
    33241 gtattgaagc agttaggatt cagttctgat ctctacgcta tgcaaagcga aatgtggttc
    33301 tacagcaata cgatggcgga caacattgct tatcgtgagc agattggtgc ggagccacgt
    33361 aaccgtggca aaccggtaga tgatatgttg ctggtagacg aaatgcagca atcgctgggg
    33421 cgcaacccgg atggtaagca tctgatcatt ctgcatacca aaggctcaca ctttaactac
    33481 acccagcgtt acccgcgcag ctttgcgcag tggaagccgg aatgtattgg tgttgatagc
    33541 ggctgtacca aagcgcagat gatcaactca tacgacaact cggtgaccta tgtggatcac
    33601 tttatttcca gcgtgattga tcaggttcgc gataagaaag cgattgtgtt ctatgcagct
    33661 gaccatggtg agtcaattaa tgaacgcgag cacctgcacg gcacgccgcg tgaactggca
    33721 ccgccggaac agttccgcgt gccgatgatg gtctggatgt cggataaata tctggaaaat
    33781 ccggtcaatg cgcaggcatt tgcgcagctg aaaaaagaag ctgacatgaa agtgccacgc
    33841 cgtcacgtag agctgtacga caccatcatg ggttgtcttg gctatacttc accggatggt
    33901 ggaattaacg aaaacaacaa ctggtgccac atcccgcagg caagagaggc ggcggctaac
    33961 taagaggcct gctgactttc tcgccgatca aaaggcattt tgctattaag ggattgacga
    34021 gggcgtatct gcgcagtaag atgcgccccg cattcggtga ttggcgcagc ctggtagcgc
    34081 acttcgttcg ggacgaaggg gtcggaggtt cgaatcctct atcaccgacc aaattcgaaa
    34141 agcctgctca acgagcaggc ttttttgcat ttgtagccta tgaggatgag aacctccggg
    34201 ggcagggagg ttcgactcga gcgaagcgag agaacgttgc gccagcaacg gcccgcaggg
    34261 cgagccacga agtggcgagt aatcctctat caccgaccaa attcgaaaag cctgctcaac
    34321 gagcaggctt ttttgcatct gtagctcatg aggaagagaa cccccggggg cggggaggtt
    34381 cgactcgacg taggtctgat aagacgcgcc agcgtcgcat caggcaacgg caccgcactt
    34441 attgtcggat gcggcgtgaa cgccttattc gatctacaca ccacacattt ccctgtcaaa
    34501 tttgcgcccc atatctcact atccattaac ggatttgtga cagaaaccat tgtctttttt
    34561 tatatttgct taaatctttt tttgcgttac ttatggctgg cgctggcatt ttccgctgtg
    34621 cgttttagcg ttcatggtat taattgctca gatatccacc ttttcctcag atttttttca
    34681 ttaaatgcat aaaacttaat caccaaatgt tgctaaatat taagctgatt gttctggttg
    34741 tgttggcgta gcacaaattt ctctgctgca aatagctgtt tgaagttttt ttaatctttg
    34801 tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg tattgacgtt ttcacattct
    34861 gttgacagat tgtaggtcac gaggggcatt ttatggagga tccgcactgt tacactgatg
    34921 ttaattagta cggcatcccc acctcataac gttgacccga ccgggcaaaa aacaaaaaag
    34981 gtcaggcagc gacaacccac tgcaaagggt taaaacaaca aacatcacaa ttggagcaga
    35041 ataatgcgta tttccttgaa aaagtcaggg atgctgaagc ttggtctcag cctggtggct
    35101 atgaccgtcg cagcaagtgt tcaggctaaa actctggttt attgctcaga aggatctccg
    35161 gaagggttta acccgcagct gtttacctcc ggcaccacct atgacgcctc ttccgtaccg
    35221 ctttataacc gtctggttga atttaaaatc ggcaccactg aagtgatccc gggcctcgct
    35281 gaaaagtggg aagtcagcga agacggtaaa acctatacct tccatctgcg taaaggtgtg
    35341 aagtggcacg acaataaaga attcaaaccg acgcgcgaac tgaatgccga tgacgtagtg
    35401 ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc ataaagtctc tggcggcagc
    35461 tacgaatact tcgaaggcat gggcttacca gagctgatca gcgaagtgaa aaaggtggac
    35521 gataacaccg ttcagtttgt gctgactcgc ccggaagcgc cgttcctcgc tgacctggca
    35581 atggacttcg cctctattct gtcaaaagaa tatgctgacg cgatgatgaa agccggtaca
    35641 ccagaaaaac tggacctcaa cccaatcggt accggcccgt tccagttaca gcagtaccag
    35701 aaagattccc gtattcgcta taaagcgttt gatggctact ggggcaccaa accgaagatc
    35761 gatacgctgg tcttctctat tactcctgac gcttccgtgc gttatgcgaa attgcagaag
    35821 aacgaatgcc aggtgatgcc gtacccgaac ccggcagata tcgcccgcat gaagcaggat
    35881 aaatccatca acctgatgga aatgccgggg ctgaacgtcg gttatctctc gtataacgtg
    35941 cagaaaaaac cactcgatga cgtgaaagtt cgccaggctc tgacctacgc ggtgaacaaa
    36001 gacgcgatca tcaaagcggt ttatcagggc gcgggcgtat cagcgaaaaa cctgatcccg
    36061 ccaaccatgt ggggctataa cgacgacgtt caggactaca cctacgatcc tgaaaaagcg
    36121 aaagccttgc tgaaagaagc gggtctggaa aaaggtttct ccatcgacct gtgggcgatg
    36181 ccggtacaac gtccgtataa cccgaacgct cgccgcatgg cggagatgat tcaggcagac
    36241 tgggcgaaag tcggcgtgca ggccaaaatt gtcacctacg aatggggtga gtacctcaag
    36301 cgtgcgaaag atggcgagca ccagacggta atgatgggct ggactggcga taatggggat
    36361 ccggataact tcttcgccac cctgttcagc tgcgccgcct ctgaacaagg ctccaactac
    36421 tcaaaatggt gctacaaacc gtttgaagat ctgattcaac cggcgcgtgc taccgacgac
    36481 cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg tgatgcacga tcaggctccg
    36541 gcactgatca tcgctcactc caccgtgttt gaaccggtac gcaaagaagt taaaggctat
    36601 gtggttgatc cattaggcaa acatcacttc gaaaacgtct ctatcgaata attaaaagcc
    36661 atacaagact gatggcaaag gcaaaactgc ctgatgcgct ccgcttatca ggcctacgaa
    36721 aactctgcaa tgtattgagt ttgcacgatt ttgtaggtcg gataaggcgt ttgcgccgca
    36781 tccggcataa acaaagcgct ctttgtcaac aatctgcata cccggtggcg ctgtgcctct
    36841 gcgtgcagcg ccatccggca gcaatacttc attccctgtt ccacaagggc gggaattgat
    36901 ttgtgagcaa tacagacacg cagttccagg ctgcgggtca ttatagagaa tccgggttat
    36961 gttgcagttt attctccgac gtttgggact cgtcatcccc acgtttatcg gtattaccct
    37021 tctcacattt gcctttgtcc acatgatccc gggcgatccg gtgatgatca tggcgggcga
    37081 gcgtgggatc tccccagagc gtcacgcgca gctgctggct gaactcggct tagataaacc
    37141 gatgtggcag cagtatctcc attacatttg gggcgtaatg cacggcgatt taggcatttc
    37201 gatgaaaagc cgcatcccgg tatgggaaga gttcgtgccg cgcttccagg ccacgctgga
    37261 acttggcgtc tgcgcgatga tttttgctac ggcagtcggt attccggttg gtgtgctggc
    37321 tgcggttaaa cgcggttcca ttttcgatca cacggcggtt ggcctggcgc tgacaggtta
    37381 ttcaatgcct atcttctggt ggggcatgat gctgatcatg ctggtttcgg tgcactggaa
    37441 cctgacgccc gtctccggtc gcgtgagcga catggtgttc ctcgatgatt ccaatccgtt
    37501 aaccggtttt atgctgatcg acaccgccat ctggggtgaa gacggtaact ttatcgatgc
    37561 cgtcgcccat atgatcttgc ccgccattgt gctgggtact atcccgctgg cggtcattgt
    37621 gcgtatgaca cgctcctcga tgctggaagt gctgggtgaa gattacatcc gcaccgcgcg
    37681 cgccaaaggg ttgacccgcc ttagggtgat tatcgtccat gcgctgcgta acgcgatgct
    37741 gccggtggtg accgttatcg gcctgcaggt gggaacgttg ctggcggggg cgattctgac
    37801 cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg attgacgcac tgcaacgccg
    37861 cgactatccg gtagtgcagg gcggcgtatt gctggtggcg acgatgatta tcctcgtcaa
    37921 cttgctggtc gatctgctgt acggcgtggt gaacccgcgt attcgtcata agaagtaagg
    37981 ggacatcatg tcacaggtta ctgaaaataa agtgattagc gcaccggtgc cgatgacccc
    38041 gttacaggag ttctggcact attttaaacg caacaaaggc gcggtcgtcg ggctggttta
    38101 cgtcgtcatc gtgctgttta tcgcgatctt tgccaactgg attgcaccct ataacccggc
    38161 ggaacagttc cgcgatgcac tgctcgcccc gccagcctgg caggaaggcg gcagcatggc
    38221 gcacttgctg ggcaccgatg acgtaggccg tgatgtgctg tcgcgtctga tgtacggtgc
    38281 gcgattgtca ctgctggttg gctgtctggt agttgtgtta tcgctgatta tgggcgttat
    38341 tctcggcctg atcgccggtt acttcggcgg cctggtcgat aacatcatca tgcgcgtggt
    38401 cgatatcatg ctggcgctgc caagtctgct gttggcactg gtgctggtgg cgattttcgg
    38461 cccgtcgatt ggtaacgccg cgctggcgct gacctttgtt gccctgccgc actatgtgcg
    38521 cttaacccgc gccgccgtgc tggtggaagt taaccgcgat tacgttaccg cgtctcgcgt
    38581 ggcaggtgcc ggggcgatgc gtcagatgtt tattaacatc ttcccgaact gccttgcgcc
    38641 tctgattgtt caggcgtcgc tcggtttctc taacgccatt ctcgatatgg ctgctcttgg
    38701 tttcctcggc atgggggcgc agccgccaac gccggagtgg ggcaccatgc tctccgacgt
    38761 gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc ccgggtctgg cgatcctgct
    38821 gacggtgctg gcatttaacc tgatgggtga cggtctgcgt gacgcgctcg atcccaaact
    38881 gaagcagtaa gaggttcgag atggcgttat taaatgtaga taaattatcg gtgcatttcg
    38941 gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag ctacagcgta aaacagggcg
    39001 aagtggtcgg gattgtgggt gagtccggct ctggtaaatc ggtcagttcg ctggcgatta
    39061 tggggctgat tgattatccg ggccgcgtaa tggcggaaaa gctggagttt aacggtcagg
    39121 atttgcagcg tatctcggaa aaagagcgcc gcaaccttgt gggtgccgaa gtggcgatga
    39181 tcttccagga cccgatgacc agccttaacc cgtgctacac cgtgggtttc cagattatgg
    39241 aagcgattaa ggtgcatcag ggcggtaata agagcactcg tcgtcagcgg gcgattgact
    39301 tgttgaatca ggtcggtatt cccgatccgg cttcgcgtct ggatgtttac ccgcatcagc
    39361 tttccggcgg catgagccag cgcgtgatga ttgccatggc gattgcctgt cggccaaaac
    39421 tgctgattgc cgatgaacca accactgcgc tggacgtgac cattcaggcg caaatcattg
    39481 aactactgct ggagctacag cagaaagaga acatggcgct ggtgttaatt acccatgacc
    39541 tggcgctggt ggcggaagcg gcacataaaa tcatcgtgat gtatgccggt caggtggtgg
    39601 aaacgggcga tgcgcacgcc atcttccatg cgccgcgtca cccgtatact caggcattgc
    39661 tgcgtgcgct gccggaattt gctcaggata aagaacgtct ggcgtcgttg ccaggtgttg
    39721 ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa cccgcgctgc ccctatgcca
    39781 ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct tgctgacggg cgtcagtcca
    39841 aatgccatta cccacttgat gatgccggga ggccgacact atgagtacgc aagaggccac
    39901 ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa cattatccgg tgaagaaagg
    39961 tatgttcgcg ccggaacgtt tggttaaagc gctggacggc gtttcgttta accttgaacg
    40021 cggcaaaacg ctggcggtag taggggaatc tggctgcggt aaatcgaccc tcggtcggtt
    40081 gctgacgatg atcgaaacgc ccaccggtgg tgaactgtat taccaagggc aggatctgct
    40141 caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa atccagattg tcttccagaa
    40201 cccttacggt tcgctgaatc cacgtaaaaa agtcgggcaa attcttgagg agccgctgct
    40261 gattaatacc agcttaagca aagaacagcg tcgggaaaaa gccctgtcga tgatggcgaa
    40321 agtcggcctg aaaaccgaac attatgaccg ctatccgcat atgttctccg gcggtcagcg
    40381 tcagcgtatc gccattgccc gaggtctgat gctcgacccg gatgtggtga ttgccgatga
    40441 gccggtttcc gcgctggacg tctcagtgcg cgcgcaggtg ctgaatctga tgatggattt
    40501 gcagcaggag ttggggctgt cttatgtctt tatctcgcac gacctgtcgg tggtagagca
    40561 cattgctgat gaagtgatgg tgatgtacct gggccgctgc gtggagaagg gaacgaagga
    40621 ccaaatcttc aataacccgc gtcatccgta cactcaggcg ctactctccg cgacgccgcg
    40681 cctgaacccg gacgatcgcc gcgagcgcat caagctcacc ggtgaactgc caagcccact
    40741 gaatccaccg ccgggctgcg cgtttaacgc ccgctgtcgt cggcgcttcg gcccctgcac
    40801 ccagttgcag ccgcagctaa aagactacgg cggtcaactg gtagcttgtt ttgctgttga
    40861 tcaggatgaa aatccgcagc gttaactcac aaaaccgggg ccgtgcgctc cggtttactc
    40921 aatcagcttc aacagtggcg acaagcatag caaaatgccg taaagcagaa tcagccaggc
    40981 tttaaacccg cgaagttttt ccagttgtgc gactttatag atcaggaaaa atggaatgag
    41041 gcacgacaca atgccatata acgggcttcc caactgaaag aacaccagca ccgatacacg
    41101 aaacgagacc caaatcgtca acgtaatgac gataaaagcg cagatcgcca gagtcagcat
    41161 gcgtgagtta attttcttgg tatcaataat tcggcttaac agattgagaa taatgccttt
    41221 aatggcctcg tggaaaccga gataaatgcc aaagaatgcg gtcagtacgg caaagatatt
    41281 cagcaccgtc gaggtgatat gaatgatatg cccagggatc acctgcgcgg ccagtgccag
    41341 tgccgagata ttttgttcaa aggcagaaac ggcttcttcg tggctaattg agaaggtaaa
    41401 cgaaaaggca aaaaatagga tcaccgcgat gagcgtgaca taactaattc ggtgggtacg
    41461 cagcgcgagc cgggttgcca gtactttatc cgcttcccgt ttgcggtagg caatattcat
    41521 cgggtttagc acctgaataa ataccgcaga aaagaagcaa aaaggaatag taagcaggac
    41581 gtcgcggaag aagacggagg cttgcgggaa ggcggttata ttggcgaaat tccagtgcgg
    41641 gatcatcgca aaaccgaaga cgacaataat ccctactttg accaccacca ttggcccgga
    41701 aatcttaaac agtaatcgtt caccaccaga cgcaattgcc accagtacgg cgaaaatggc
    41761 gactttataa agtagagatt gtgaaagatc ggcatcggtt aaaccgaagg ttttcaggta
    41821 cgaggcgctg tcgaaaacca cggagagaga gtagataaaa atcccgtgga taatcatcaa
    41881 aaagtagata accccgagga aaattcccca gttcttcccc aggtaatgac tgataatatc
    41941 ggtgtagtca ttacaggaat cgctttcaga aagggttttt aaataaatgt cctgcactac
    42001 ccaggtggca ggataagcaa tgatcgctgc ggtaataaat acccaaattc ccttcaagcc
    42061 aatttgtact ggcatcagca cggttccggc accaatcgcc atgcctatgc ataataaaac
    42121 ccagccgaag tcgtagcgtg taaacggcat tttctgatcg tgtttcggta gtgtgttgtg
    42181 ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa cccagtttct
    42241 cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc aacagattca
    42301 ctggggaggt cgcgaggagg cgcaattcta caaaattgcg catttcacta ccagtctgga
    42361 gtcagcccgg atatgaactg caactaatgc aaataaagaa attaagctgg aaggcatcac
    42421 gcaatgagga agattgccgg gtgctgacgg taaaaagcaa tcgtaaaatc agcggcaagc
    42481 cggaaaggtt ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattggttgc
    42541 tggagagaga aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggctaat
    42601 cttgaaccta gaccactcaa gaatagccgc gaaacgttgt cattacaaca caggcggcta
    42661 tatgacgctc gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcatagc
    42721 tggcattctt gccagtctga tcgtgaactg gctgaacaag cggaagtaac gtgtcagacg
    42781 ggcgtcaggc tgccgtaatg gcaatttgcg cccggatcag gccgcaggga taaaactctg
    42841 cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga taatattctg cggaatgcac
    42901 agcacagagt gaagaagtga cggatttcgg agctgaaaaa cagtcgtaga atcagcggca
    42961 agccggaaag gttccggtga ggcgcaatgt tgcgggggct taatccctgg tggcattggt
    43021 tgctggagag agaaaacccc cgcatattgc aggtatgcac ctgacaacat cacgggggcc
    43081 attcttgaac ctacacaact caagaatagc cgcgaaacgt tgtcattaca actcaggcgg
    43141 ctatatgacg ctcgcagagc tgggcatggc cttctggcat gatttagcgg ctccggtcat
    43201 tgctggcatt cttgccagca tgatcgtgaa ctggctgaat aagcggaagt aacgtgtcag
    43261 acgggcgtca ggctgccgta atggcaattt gcgcccggac caggccgcag ggataaaact
    43321 ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt ctgcggaatg
    43381 cgcagcacag agtgaagaag tgatggattc cggaacagaa aaacagtcgt agaatcagcg
    43441 gcaagccgga aaggttccgg tgaggcgcaa tgttgcgggg gcttaatccc tggtggcatt
    43501 ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa caccacgggg
    43561 gccattcttg catctagacc attcaagaat agccgcgaaa cgttgtcatt acaactcagg
    43621 cggctatatg acgctcgcag agctgggcat ggccttctgg catgatttag cggctccggt
    43681 cattgctggc attcttgcca gcatgatcgt gaactggctg aataagcgga agtaacgtgt
    43741 cagacgggcg tcaggctgcc gtaatggcaa gttgcgcccg gatcaggccg cagggataaa
    43801 actctgcggc ctttttcgta agaaaaatgc aacggttgca agcggataat attctgcgga
    43861 atgcgcagca cagagtgaag acgtgacgga ttccggagca gaaaaacagt cgtagaatca
    43921 gcggcaagcc ggaaaggttc cggtgaggcg caatgttgcg ggggcttaat ccctggtggc
    43981 attgtttgct ggagagagaa aacccccgca cgttgcaggt atgcacctga caacaccacg
    44041 ggggccattc ttgaacccaa acaactcaag aatagccgcg aaacgttgtc attacaactc
    44101 aggcggctat atgacgctcg cagagctggg catggccttc tggcatgatt tagcggctcc
    44161 ggtcattgct ggcattcttg ccagcatgat cgtgaactgg ctgaacaagc ggaagtaacg
    44221 tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc ccggatcagg ccgcaggggt
    44281 gaaactctgc ggcctttttc gttcttattg cgggtaaggc acccagtcgc cgccgttcag
    44341 gcgaacgtac ggtttatcct ggtattgaat aactactgca tttgagttct cggagaccgg
    44401 tgctgtttgt ggcaacccac tggtgagttt tttccagtca acattgtctt cggagaaaat
    44461 cttgccatca agaacgcgaa ccaccagatc ggagatagcc aggaagctgc tcggttggtc
    44521 gatgacaatc ggtgccccct gatgtggtgc cttcatgccg aagaatttca ccccaacggg
    44581 gacgtctgtg atagacgggc tagggatatc acgtaggcca gatacctgca ttctgtcgcc
    44641 cttcagcgcg ccgccgtgtt ccggcaccac gaccaccatc actttacgac ccgatttctc
    44701 cagttcagta aagaaggcgt ccagttcatc aaagaatttc tgcgcccgcg ctttgtaatc
    44761 cgctgttttg ctgacgcccg gataatggtt gccgtcatgc agtggaagcg tgttgtagaa
    44821 cgtggcacta cggctgtttt tatctttttc ggtaatgtcc agccagcggt taagcacggc
    44881 ggtatcgtca taaaccggcg aaccatcaaa gcccagcaaa ataaccggca gatttgtttg
    44941 atccatcaat tcggtctgca tgccgccatt ttcgcgaact tctttcaaaa aaccgccgaa
    45001 ctggccgtta tgtcccatca tcaggtgctg ggtaaagccc agtttcgaca ggttatcaaa
    45061 cagatagcag tcgttatttg ccggttgata cagattagtg tgcgaagtct gcccgcagct
    45121 ggcgcgcagt aaacggatcg ccgccgggcc actgtaggag gtggcggagt taaagttctt
    45181 gaactcaata tcgaaatgcg accacagcgg atgcgacatc aacccggcgg cttcaatatc
    45241 cgaccaggaa agcgaacaga tattaatcac cagtagttca aatggctgag catcagcggg
    45301 cagcgaagac gggaaggtcg atttacgttt cgcctccgcg ttatagaaat tatttagcca
    45361 ggcgttaagg ttcgccgttg ttggcggtgc agtttgtgcg ggcatatcac ccactaccgg
    45421 tgcgccaccc gtcgccgcaa cggttgccgc tgcgttacca cccgtcgttg ttacagtggt
    45481 cgtcggttga ccggctggcc acaaggagaa acttggtccc gccagggtaa gtacgttcag
    45541 ccatagcaga atggcaacca caaaaacggt aatgcgaatc cattgtgaca ggaataacca
    45601 ggccacgaat aaaacaaaaa tggccccaat catctgccag ttaataaagc gtgtgacaag
    45661 gtcgattaaa taatcggtac tgaaccccgc cacctgcgaa ccctggctca ttatgctttc
    45721 cgggccaggc aaccaggtgt catgccagaa caaagcaaag ccgatcggca gggcaatcca
    45781 gtggcgcaag cgatgcaggc tgtagcgcgg aatgggcatc agcagaaacg cggcaaagac
    45841 caaattgagg agcggatgga agttaagata tcccgcccac aacaggccga acttaaccag
    45901 aaaatagaag ttccagccgg aaaggccgcg ccagtattgc cagagggaag aaggcatggc
    45961 ggtattttgc gtaaattgag tcattttttg gttgccctgg ctttttccgt gcggcgtaac
    46021 gtcccggccg gtttaacata acgaggttta gcaaagaaca aacgtaaggt gtcgcgaatg
    46081 cgtcgcaaag agtgccgcgc cagatagccc agcgggaaaa atatcagtgc gcaaacgaca
    46141 ataatttcaa tgatatcgct gatggtcatc atgatgagcg ctccacagca tcatctaaca
    46201 gtcgcattgg ttctggtatt cgtcgccagt ggcgaccatc gtgctcggca ttgatgaccg
    46261 gtttagaact ttgcgttaaa ggcagcggca tgccccattg ttctggggca agcaggcgca
    46321 tctgcaccag ctcggcactg atttgatcat cttcaaacca gaccatacgg tttgagaaaa
    46381 tgtcgccagt aggcaatggg aaaatatgat tcaacgcggt atccagatcg ttaatccgac
    46441 agaatgagag aaacagcacc agccgattac cgccaatggt catgatatcg ccggtgcgat
    46501 tagggcgaca tagcgtcagg gcttgctcaa cgcggatacc cggtaccgga cgtagggcaa
    46561 ccagaacgcc tttaccgtgg gcaggtaata gagggttatt catcatgttg ttgacggcat
    46621 tacagaacac atcccacttc tggaaaccac gcagtttgag cggctgggtc attgacagca
    46681 aggtagtgat atcttccggc acatagcgac taaacttctg cccttgcacg ctttcgatca
    46741 tcgtcagaca gcgggagagt ggcgcattcc acgggataac catatttgca ccgcaggcca
    46801 ataataaacg ttcatcggtg gcgcgcaggc tagcggtatt ttcccgtacg aggattttca
    46861 tcgcactacc gcgctggcga cgcagagtat gaatgctgcg ggccagtggc tcgatttgcg
    46921 cattttgctg taaagaaaag accaccgtcg ccgcctgagc ggtacgggct tcattgaaca
    46981 ggacttcgtt attgttgaac agttgccagt gttccgatag cggcggcgca ccttccagta
    47041 cagcaacatt actcagaatg cgtttttcgt cgctgcgtgg ttggatttcc gcctcctggt
    47101 gttgaactaa tgtccagcaa ccattttgtt gctgaacgct aagctgctga cgggcgctga
    47161 cccctttttc gttgcaccag aaagcaatat ccagcaaatg ttggtcgccc tgaaaacgca
    47221 aactggcaag accaaaaagt gaacggtatt cctcaagtaa caatgaaaat tgtttatcgt
    47281 tattatttcc ggaattaatt accaaaagcg aacaatggtt tagccggctc catttattca
    47341 ttttatccaa ccatgagcga agccgctcgg caggaatgtt ttgccatgcg ttatttgcgc
    47401 aaacaagaat aaaaaggtaa ttatggggat caatagaaca ctgcaaatca cgggccaaat
    47461 agtatagacc tttttcatga tttagcattg aaaataatgt tatttttccc ggcccttgag
    47521 aatcatctaa ttgaaagatt ttcgctggat cgctgtccat gctaatgacc gcgacgtgtg
    47581 cggtttcagc ctgggatgca attgtttgat tcgccagact gatagcatct tcatggcgat
    47641 cgacgttaaa ccaccagacg ccgcctgctg gcatatgtcg cagctcatcc cataatgatg
    47701 agataccgat agagaataca gggtccacaa tgtccctcat gtcaacattt tgtctgatta
    47761 tcagtttact agcgaaagct aagaaataaa cctaccattg aaattaaaac ttatcagatt
    47821 tagcatgtga atcaatttag caggtcacaa gacatgtgat tttagtcttt tcgtttttaa
    47881 ctttttctgt tgcgtaacgg aaagtcaaaa agtgagcaca ttcccgtctc gccgtaaaca
    47941 ataaaccgac gaaatgctca cagttagaat tgtctcatga acggtacggt tatttcatag
    48001 ggatcaagaa aaatgaataa caatgaacca gatactctgc ctgatcccgc gataggctat
    48061 atcttccaga atgatatttt ggcgttaaag caggcatttt cactgcctga tattgattat
    48121 gccgatattt cccaacgcga acagttggcc gcggcattaa aacgctggcc gttgctggca
    48181 gagtttgcgc aacaaaagta ggggattggt gaatggccgt act
//
LOCUS       DAZKJR010000019        48046 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKJR010000019 DAZKJR010000000
VERSION     DAZKJR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.019"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..138)
                     /locus_tag="ACHOWS_001125"
     CDS             complement(<1..138)
                     /locus_tag="ACHOWS_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HFQ0046744.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SY"
     gene            complement(344..670)
                     /gene="ynfA"
                     /locus_tag="ACHOWS_001126"
     CDS             complement(344..670)
                     /gene="ynfA"
                     /locus_tag="ACHOWS_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFQ0046745.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            805..1146
                     /gene="ynfB"
                     /locus_tag="ACHOWS_001127"
     CDS             805..1146
                     /gene="ynfB"
                     /locus_tag="ACHOWS_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFQ0046746.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1181..1741
                     /gene="speG"
                     /locus_tag="ACHOWS_001128"
     CDS             1181..1741
                     /gene="speG"
                     /locus_tag="ACHOWS_001128"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFQ0046747.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(1744..2454)
                     /gene="ynfC"
                     /locus_tag="ACHOWS_001129"
     CDS             complement(1744..2454)
                     /gene="ynfC"
                     /locus_tag="ACHOWS_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFQ0046748.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2562..2867
                     /gene="ynfD"
                     /locus_tag="ACHOWS_001130"
     CDS             2562..2867
                     /gene="ynfD"
                     /locus_tag="ACHOWS_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFQ0046749.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3066..5492
                     /gene="ynfE"
                     /locus_tag="ACHOWS_001131"
     CDS             3066..5492
                     /gene="ynfE"
                     /locus_tag="ACHOWS_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFQ0046750.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLVNENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            5553..7976
                     /gene="ynfF"
                     /locus_tag="ACHOWS_001132"
     CDS             5553..7976
                     /gene="ynfF"
                     /locus_tag="ACHOWS_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFQ0046751.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            7987..8604
                     /gene="ynfG"
                     /locus_tag="ACHOWS_001133"
     CDS             7987..8604
                     /gene="ynfG"
                     /locus_tag="ACHOWS_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFQ0046752.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            8606..9460
                     /gene="ynfH"
                     /locus_tag="ACHOWS_001134"
     CDS             8606..9460
                     /gene="ynfH"
                     /locus_tag="ACHOWS_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFQ0046753.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWYNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            9503..10117
                     /gene="dmsD"
                     /locus_tag="ACHOWS_001135"
     CDS             9503..10117
                     /gene="dmsD"
                     /locus_tag="ACHOWS_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFQ0046754.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            10276..11568
                     /gene="clcB"
                     /locus_tag="ACHOWS_001136"
     CDS             10276..11568
                     /gene="clcB"
                     /locus_tag="ACHOWS_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFQ0046755.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(11521..12216)
                     /gene="bioD"
                     /locus_tag="ACHOWS_001137"
     CDS             complement(11521..12216)
                     /gene="bioD"
                     /locus_tag="ACHOWS_001137"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFQ0046756.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(12341..13561)
                     /gene="mlc"
                     /locus_tag="ACHOWS_001138"
                     /gene_synonym="dgsA"
     CDS             complement(12341..13561)
                     /gene="mlc"
                     /locus_tag="ACHOWS_001138"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFQ0046757.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(13696..14589)
                     /gene="ynfL"
                     /locus_tag="ACHOWS_001139"
     CDS             complement(13696..14589)
                     /gene="ynfL"
                     /locus_tag="ACHOWS_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0046758.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMMDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            14696..15949
                     /gene="ynfM"
                     /locus_tag="ACHOWS_001140"
     CDS             14696..15949
                     /gene="ynfM"
                     /locus_tag="ACHOWS_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0046759.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYVGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            complement(15999..16118)
                     /locus_tag="ACHOWS_001141"
     CDS             complement(15999..16118)
                     /locus_tag="ACHOWS_001141"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFQ0046760.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            16374..16682
                     /gene="asr"
                     /locus_tag="ACHOWS_001142"
     CDS             16374..16682
                     /gene="asr"
                     /locus_tag="ACHOWS_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFQ0046761.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            16775..16858
                     /locus_tag="ACHOWS_001143"
     CDS             16775..16858
                     /locus_tag="ACHOWS_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046762.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            16958..17779
                     /gene="ydgD"
                     /locus_tag="ACHOWS_001144"
     CDS             16958..17779
                     /gene="ydgD"
                     /locus_tag="ACHOWS_001144"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFQ0046763.1"
                     /translation="MRRTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(17818..18147)
                     /gene="mdtI"
                     /locus_tag="ACHOWS_001145"
     CDS             complement(17818..18147)
                     /gene="mdtI"
                     /locus_tag="ACHOWS_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFQ0046764.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(18134..18499)
                     /gene="mdtJ"
                     /locus_tag="ACHOWS_001146"
     CDS             complement(18134..18499)
                     /gene="mdtJ"
                     /locus_tag="ACHOWS_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFQ0046765.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            18910..19944
                     /gene="tqsA"
                     /locus_tag="ACHOWS_001147"
     CDS             18910..19944
                     /gene="tqsA"
                     /locus_tag="ACHOWS_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFQ0046766.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLQQYRNSIM
                     TPLKAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(19969..21357)
                     /gene="pntB"
                     /locus_tag="ACHOWS_001148"
     CDS             complement(19969..21357)
                     /gene="pntB"
                     /locus_tag="ACHOWS_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFQ0046767.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATILGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(21368..22900)
                     /gene="pntA"
                     /locus_tag="ACHOWS_001149"
     CDS             complement(21368..22900)
                     /gene="pntA"
                     /locus_tag="ACHOWS_001149"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFQ0046768.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFTAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            23424..24368
                     /gene="ydgH"
                     /locus_tag="ACHOWS_001150"
     CDS             23424..24368
                     /gene="ydgH"
                     /locus_tag="ACHOWS_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFQ0046769.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            24554..25936
                     /gene="ydgI"
                     /locus_tag="ACHOWS_001151"
     CDS             24554..25936
                     /gene="ydgI"
                     /locus_tag="ACHOWS_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0046770.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWS
                     ITGAGILLLTFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            25973..26695
                     /gene="folM"
                     /locus_tag="ACHOWS_001152"
     CDS             25973..26695
                     /gene="folM"
                     /locus_tag="ACHOWS_001152"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFQ0046771.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIISYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(26692..27027)
                     /gene="ydgC"
                     /locus_tag="ACHOWS_001153"
     CDS             complement(26692..27027)
                     /gene="ydgC"
                     /locus_tag="ACHOWS_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFQ0046772.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            27156..27875
                     /gene="rstA"
                     /locus_tag="ACHOWS_001154"
     CDS             27156..27875
                     /gene="rstA"
                     /locus_tag="ACHOWS_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFQ0046773.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILQE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            27879..29180
                     /gene="rstB"
                     /locus_tag="ACHOWS_001155"
     CDS             27879..29180
                     /gene="rstB"
                     /locus_tag="ACHOWS_001155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFQ0046774.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAVESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            29256..30185
                     /gene="tus"
                     /locus_tag="ACHOWS_001156"
     CDS             29256..30185
                     /gene="tus"
                     /locus_tag="ACHOWS_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFQ0046775.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKTAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(30182..31585)
                     /gene="fumC"
                     /locus_tag="ACHOWS_001157"
     CDS             complement(30182..31585)
                     /gene="fumC"
                     /locus_tag="ACHOWS_001157"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFQ0046776.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRVFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(31728..33374)
                     /gene="fumB"
                     /locus_tag="ACHOWS_001158"
     CDS             complement(31728..33374)
                     /gene="fumB"
                     /locus_tag="ACHOWS_001158"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFQ0046777.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            33573..34748
                     /gene="manA"
                     /locus_tag="ACHOWS_001159"
     CDS             33573..34748
                     /gene="manA"
                     /locus_tag="ACHOWS_001159"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFQ0046778.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISECYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            34849..36357
                     /gene="ydgA"
                     /locus_tag="ACHOWS_001160"
     CDS             34849..36357
                     /gene="ydgA"
                     /locus_tag="ACHOWS_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFQ0046779.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(36402..37667)
                     /gene="uidC"
                     /locus_tag="ACHOWS_001161"
     CDS             complement(36402..37667)
                     /gene="uidC"
                     /locus_tag="ACHOWS_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFQ0046780.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(37706..39079)
                     /gene="uidB"
                     /locus_tag="ACHOWS_001162"
     CDS             complement(37706..39079)
                     /gene="uidB"
                     /locus_tag="ACHOWS_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFQ0046781.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            complement(39076..40887)
                     /gene="uidA"
                     /locus_tag="ACHOWS_001163"
     CDS             complement(39076..40887)
                     /gene="uidA"
                     /locus_tag="ACHOWS_001163"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0046782.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQEIRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(41274..41864)
                     /gene="uidR"
                     /locus_tag="ACHOWS_001164"
     CDS             complement(41274..41864)
                     /gene="uidR"
                     /locus_tag="ACHOWS_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFQ0046783.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLSREASFAQGLRAMIGGILTAS"
     gene            complement(42085..42852)
                     /gene="hdhA"
                     /locus_tag="ACHOWS_001165"
     CDS             complement(42085..42852)
                     /gene="hdhA"
                     /locus_tag="ACHOWS_001165"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFQ0046784.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(42964..43992)
                     /gene="malI"
                     /locus_tag="ACHOWS_001166"
     CDS             complement(42964..43992)
                     /gene="malI"
                     /locus_tag="ACHOWS_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFQ0046785.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            44167..45759
                     /gene="malX"
                     /locus_tag="ACHOWS_001167"
     CDS             44167..45759
                     /gene="malX"
                     /locus_tag="ACHOWS_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFQ0046786.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            45769..46941
                     /gene="malY"
                     /locus_tag="ACHOWS_001168"
     CDS             45769..46941
                     /gene="malY"
                     /locus_tag="ACHOWS_001168"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFQ0046787.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFASAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            47045..48046
                     /gene="add"
                     /locus_tag="ACHOWS_001169"
     CDS             47045..48046
                     /gene="add"
                     /locus_tag="ACHOWS_001169"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0046788.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
BASE COUNT        11906 a        12425 c        11708 g        12007 t
ORIGIN      
        1 ataagaggcc acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc
       61 gtcctcagtg gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc
      121 agcctttacg atcttcatgt tcgattcctt gcatcgcttg tcgtgatgca tgaaatctac
      181 gcaactgagc tactaccata caagtatgaa gatcaaaaaa agccggagtg atcacaaaaa
      241 aaggcgtata tttgcgccgt gaatggttga caaaagatga aatagaatac cttttgtcag
      301 ctgacacttc ctcgtatctt attgataaaa gggatttatg ttcctacgtg cgcccccagc
      361 ccgcaacaat gatcaacatg ccgcaaagcg caatcaacgc ccccgtccag tcataaagac
      421 tcagtttcac gccatccaca acgcgcagcc acatcaacgc cgtgcagaca taaacgccac
      481 cataagcggc gtaaacacgc ccactcgccg caggatgcaa cgttaacaac cagacaaaca
      541 gcgccagtga aatccccgcc ggaagcaaca gccagatact ggcgtttcgt tttaaccaca
      601 accagggcag aaagcatcca ataatttcac acagcgcagt agcaaaaaat agtaacgttg
      661 ttttaatcat ctttgtcact tattgacatc atgtatagtt ataggacgac ataatatcat
      721 caatataaac atcctcctgg tacgttttgc gttcgcagtg gatggtgtag aatcaccttt
      781 aatcattcat acagggaatg aattatgaaa attactctca gcaaacgaat tggcctgctc
      841 gcttttctgc tgccttgcgc actggcattg agcacaactg ttcatgccga aactaacaaa
      901 ctggtgattg agtctggcga cagtgcacaa agccgccagc acgccgctat ggaaaaagag
      961 caatggaatg acacgcgcaa tctgcgccag aaagtgaata aacgcactga aaaagagtgg
     1021 gataaagccg acgccgcttt tgataaccgc gataaatgtg agcaaagcgc caacatcaat
     1081 gcctactggg agccaaatac tttgcgctgc ctggaccgtc gaactggccg cgttattacc
     1141 ccctaacctg ttattgattt aaggaatgta aggacacgtt atgccaagcg cccacagtgt
     1201 taagctacgc ccgctggagc gtgaagattt acgctatgta catcaactcg acaataacgc
     1261 cagtgtgatg cgttactggt ttgaggaacc ctacgaagcc tttgttgaac tctctgatct
     1321 gtacgataag catattcacg atcagagcga acggcgcttt gtggtggaat gtgacggcga
     1381 aaaagccggt ctggtggagc tggtggaaat taaccatgtt catcgccgcg cagaatttca
     1441 gataattatc tccccggaat atcaggggaa aggtctggca acgcgtgccg caaaattagc
     1501 aatggactat ggcttcaccg ttctcaatct ctataagctg tatctgatcg tcgataaaga
     1561 gaatgaaaaa gcgattcaca tttaccgcaa gcttggcttt acggttgaag gtgaattgat
     1621 gcatgagttc tttattaatg gtcaatatcg taatgccatt cgcatgtgca tattccagca
     1681 tcagtatctg gcagagcaca aaacaccggg tcagactctc ctgaagccga ccgcacaata
     1741 gcattaataa taatcgatag tatttttgat ggtgtaaacc cgttcgacgg cgggttttac
     1801 tccttcatca acaatgatta gctgacagtc caccggattg gcgtgactgt catattcaca
     1861 gctctgtttt acattaccaa ccctttgatt attcagtaaa gtaaccgctg tgtaatctaa
     1921 ttttttgatc ggatccgttg atggcgtagc gctgacagat aatgttttgt cgttactttt
     1981 cgtggtttta cccagcggat acccctgatc atcatagcga tattccatct gcatctgttt
     2041 gctgctggct ttaatcacaa agccattatc atcggtttcc cagctcaccc cggcagaagg
     2101 taattctgct agctggcatt ttccctgcaa acgaactctc ttctccagtg tctgggcatc
     2161 acggtaataa ttggcgtcca gtaccagggc aaccacggta ttattttcca gatccagtaa
     2221 ttcgagtgaa tcaaaacagc cttcttccga caaagtcccc gaaacacgtt tcgtcacttc
     2281 accttgctca tccattaatg tctgagtgaa atcttttacc ggaccacgca gcggatcaaa
     2341 atcgaattca ttagagaaac tggccatctc aggggtaaat gaaagtgtta cctctgtgcg
     2401 gtcacatcct gtgaggaata tcgcgagtaa gcatggcagt aatttgtatt tcacaacagt
     2461 caccagagag tagagatgat tctcaatcat agtagcaaat acagtacttt acacgttaaa
     2521 tgctatgctt aaagaagtta tcttcgcgta aggagcttat gatgaaactc tcaacctgct
     2581 gtgccgcgct tctgctcgcc ctcgcctcac ccgcggtact cgccgcgccg ggatcctgtg
     2641 agcgcataca gagcgatata tcacagcgca ttatcaataa tggcgtaccc gaaagcagct
     2701 tcacgttaag tattgtaccc aatgaccagg ttgatcagcc tgattcccag gtcgtcgggc
     2761 attgcgctaa tgatacgcat aaaattctct acacccgcac caccagcggt aacgtctctg
     2821 ctcccgcgca gtctacccag gatggcgcgc ctgccgaacc gcagtaatac attatcgact
     2881 gcacgccgga tacgacacat ccggcgattt gaacatacaa cataatccca ccttattact
     2941 catacccttc tattgatatg gattaataat tcttaaccca aaataggtag actccctcta
     3001 ttgttagcgc gctaaatatt caatatataa acttttatat aacgataaag aacagggagt
     3061 gagttatgtc caaaaatgaa caaatggtgg gcatcagccg cagaacactc gttaaatcta
     3121 ccgcgatagg ttctctggcg ctggctgcag gcggtttttc tttaccgttc accctgcgca
     3181 gtgcagcggc agcggtacag caggccagcg agaaagtggt ctggggtgcc tgttccgtca
     3241 actgtggtag ccgctgtgca cttcgtctgc atgttaaaga taatgaagtg acctgggtgg
     3301 aaactgacaa caccggcagc gatgagtatg gcaatcatca agtacgcgcc tgtttgcgcg
     3361 gtcgctccat ccgtcggcgt attaatcatc ccgaccgctt gaattaccca atgaaacgcg
     3421 tgggcaaacg cggcgaaggc aaattcgaac ggattagctg ggatgaagcc ctggatacta
     3481 tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaggctgta tatattcagt
     3541 actcttcagg gatcgttggc ggcaatatga cccgctcttc gccatccgcc tcggcggtca
     3601 aacgcctgat gaactgctac ggcggttcac tcaaccagta tggctcctac agcaccgcgc
     3661 aaatctcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc accacggata
     3721 ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggctgaaacc cgcatgagcg
     3781 gcggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgca aaaatgattg
     3841 ttatcgatcc gcgatatacc gatacggctg ctggtcgtga agatgaatgg cttcctattc
     3901 gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattggtt aacgaaaatc
     3961 tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa accttacccg
     4021 cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt gatgataaaa
     4081 cagcgaaaac gccgcagtgg gcttcgcaaa ttacaggtat cccggtagac cgtatcatca
     4141 aactggcgcg tgaaattggc acagcaaaac ccgcttatat ctgccagggc tgggggccgc
     4201 aacgccaggc aaacggcgag ctgacggcac gcgctattgc tatgttgcct attttgacgg
     4261 gcaatgtcgg catcagcggc ggaaatagtg gtgcgcgtga atcgacctac accattacca
     4321 tcgaacgcct gccggtgctg gataatccgg tcaaaacgtc aatctcctgc ttcagttgga
     4381 cagatgctat cgatcatggc ccgcaaatga cggcaatccg cgacggcgtc cggggcaaag
     4441 ataaactgga tgtgcccatt aagttcatct ggaactacgc gggaaatacc ctggttaatc
     4501 agcattctga catcaacaaa acgcatgaaa ttctgcagga cgaatcgaaa tgcgaaatga
     4561 ttgtggtcat cgaaaacttt atgacctcat cggcaaaata tgccgacatt ctgctgccag
     4621 acctgatgac cgttgagcag gaagatatta ttcctaacga ctacgccggt aacatgggat
     4681 atctcatttt cctccagcct gtcaccagcg aaaaattcga acgcaaacca atttactgga
     4741 tcctgagtga agtcgcgaaa cgtcttggac ctgacgtcta tcaaaagttc actgaaggtc
     4801 gcacgcagga acaatggtta caacatctgt acgccaaaat gcttgccaaa gatccggcgt
     4861 taccgtctta cgacgaatta aaaaaaatgg gtatctataa gcgtaaagat cccaatggtc
     4921 attttgtcgc ctacaaagca tttcgtgacg accccgaggc aaatccactt aaaacgcctt
     4981 ccggtaagat tgaaatttat tccagcaagc tggcggaaat tgcccgtacc tgggaactgg
     5041 aaaaagatga agtgataagc ccactgccgg tttatgcctc aacctttgaa ggctgggact
     5101 cccctgaacg tagtaccttt ccactgcaac tgtttggttt ccattacaaa tcccgtactc
     5161 actcgaccta cggcaacatt gatgtcctga aggctgcctg ccgtcaggag gtgtggatca
     5221 accctataga cgcacagaaa cgtgggattg ccaacggcga tatggtgcgg gtgtataacc
     5281 atcgtggcga agttcggcta ccagccaaag tgacaccacg cattctccct ggggttagcg
     5341 ctatgggcca gggagcctgg cacgaggcca atatgtctgg cgataaaatc gaccatggcg
     5401 gctgtgtgaa tacgctaacc actctgcgcc cttcaccact ggcgaaggga aacccgcagc
     5461 acactaatct ggtcgagatc gaaaaaatat aacccactac aaccataaat tctggcatga
     5521 cattttgttt gaaaagcaat aagtgagtaa tgatgaaaat ccataccaca gaggcgctca
     5581 tgaaggctga aatcagccgt agaagtctga tgaaaacctc cgcacttggc agtctcgcgc
     5641 tggcaagcag tgctttcact ctgccatttt cccaaatggt ccgagcggca gagacaccgg
     5701 tagaagagaa agcggtctgg agttcctgca ccgttaactg cgggagccgc tgtctgttac
     5761 gtttgcatgt gaaagatgac accgtgtact gggtggagtc tgatacgaca ggagacgacg
     5821 tttacggtaa tcatcaggtt cgggcatgtt tacgcgggcg ctctatccgc cgacggatga
     5881 atcatcctga taggttgaaa tatcccatga agcgcgtcgg caagcgcggt gaaggtaaat
     5941 ttgaacggat aagttgggat gaagccctgg ataccatcag tgataatctt cggcggatcc
     6001 ttaaagatta cggcaatgag gctgtacatg tcctgtacgg aacaggcgta gatggcggaa
     6061 acatcaccaa ctcgaacgtc ccgtaccgtc tgatgaactc ttgcggtggt tttctcagtc
     6121 gctatggcag ctacagtacc gctcaaatca gtgccgcaat gagttatatg ttcggtgcca
     6181 atgatggcaa cagcccggat gatatcgcca atacgaaact ggtcgttatg ttcggaaata
     6241 acccggcaga aacccggatg agcggcggtg gtgtcactta ctacgtcgag caagcccgag
     6301 aacgttcaaa cgcacgcatg atcgtcatcg atccacgtta taacgacaca gctgccgggc
     6361 gtgaagatga atggctgcca attcgcccag gcaccgatgg cgcactggcc tgtgcgattg
     6421 cctgggtact gattactgaa aacatggtcg atcagccatt tctcgacaaa tattgtgttg
     6481 gttacgatga aaaaacgctg cccgccaacg caccacgtaa cgcgcattat aaagcctata
     6541 ttctgggcga agggcctgac ggcatagcta aaacgccgga atgggcagca aaaatcacca
     6601 gcatcccggc agaaaaaatt atccagttgg cacgagagat cggttcagca aaacctgctt
     6661 atatttgtca gggttggggg ccacaacgac actccaacgg cgaacaaacg tcccgcgcta
     6721 ttgccatgct ttccgttctc accggtaacg tcggcataaa cggcggcaac tcaggtgtac
     6781 gcgaaggtag ctgggatctg ggggtagaat ggttcccgat gcttgagaat cctgttaaaa
     6841 cgcagatttc cgtctttaca tggacagatg ccatcgacca cggtacggaa atgaccgcga
     6901 cccgtgatgg tgttcgtgga aaagaaaaac tggatgtccc catcaagttt ttatggtgct
     6961 acgccagtaa cacattgatc aatcaacatg gcgacatcaa tcacacccat gaggtgcttc
     7021 aggatgacag caagtgcgag atgattgttg gcattgacca cttcatgact gcctcggcta
     7081 agtattgcga tatcctgttg cccgacctga tgccaacaga gcaagaagac cttatctctc
     7141 acgaatctgc cgggaatatg ggctatgtga tcctcgccca acccgcaacc tcagcaaaat
     7201 ttgaacgcaa acccatctac tggatgctga gcgaagttgc caaacgctta ggaccagacg
     7261 tttatcaaac ctttactgaa ggccgcagtc agcacgaatg gatcaaatat ctccatgcga
     7321 aaacgaagga acgtaaccct gagatgcccg actacgagga gatgaaaacg accgggatct
     7381 ttaagaaaaa atgcccggaa gaacactacg tcgctttccg cgcattccgt gaagatccac
     7441 aggcaaaccc gttgaaaaca ccttcgggga aaatcgaaat ttattctgaa cgactggcga
     7501 cgattgcgga tacctgggaa ttgaaaaaag atgaaattat tcatcccctt cctgcgtata
     7561 ccccaggttt tgatggctgg gacgatcccc tgcggaaaac ctatccactg cagctaacgg
     7621 gcttccacta caaagcgcgt acccactcca gctacggcaa tattgatgtg ttacagcagg
     7681 cctgcccaca agaggtgtgg atcaacccta ttgatgctca ggcacgcggt atccgtcatg
     7741 gcgataccgt gcgggtattt aacaataatg gagaaatgct gattgccgca aaagtgactc
     7801 cgcgtattct gcctggcgtt accgccatcg ggcaaggtgc gtggcttaaa gcggatatgt
     7861 ttggtgaccg ggtcgatcac ggcggcagta tcaatattct gacctctcac cgcccttcac
     7921 cgctggcaaa gggaaacccg tcgcacagca atcttgtcca gatcgaaaag gtttaaggag
     7981 tagcccatga ccacacaata tggatttttt atcgattcca gccgctgtac tggctgtaaa
     8041 acctgcgaac tggcgtgcaa agattttaaa gatcttggcc cggaagtcag tttccgccgt
     8101 atttacgaat atgctggcgg cgactggcag gaagataatg gcgtctggca ccaaaacgtg
     8161 ttcgcttatt atctctccat ctcctgtaac cattgcgatg accccgcatg tacaaaagtc
     8221 tgccccagcg gcgcaatgca taagcgtgaa gatggctttg tggtggttga tgaggatgtt
     8281 tgtatcggct gccgctactg ccacatggct tgtccgtacg gcgcgccaca gtacaatgct
     8341 gaaaaagggc acatgacgaa gtgcgatggt tgttattcgc gcgtcgccga ggggaaacaa
     8401 cccatatgtg tcgaatcctg cccgctgcgt gcacttgagt tcggtccaat tgaagagctg
     8461 cgccagaagc acggcactct ggctgccgtc gcgccactac cgcgcgcgca tttcacaaaa
     8521 cccaatatcg ttatcaaacc taacgccaac agccgcccga ccggcgatac cactggttat
     8581 ctggctaatc cggaggaagt gtaaaatggg aaatggatgg catgaatggc cactggttat
     8641 ctttaccgta ttgggtcaat gcgtagtagg cgcgctgatt gttagcggaa ttggctggtt
     8701 cgccgccaat aatgacgcag accgtcagcg tattgtccgt ggcatgtttt tcctctggct
     8761 gttgatgggc atcgggttta ttgcctctgt catgcacctg ggatcgccac tgcgagcgtt
     8821 taactcgctg aatcgaatcg gagcttctgg tctgagtaac gagattgctg ccggttccat
     8881 attttttgcc gttggcggat tatggtggct ggtggcagtc atcggtaaaa tgccgcaagc
     8941 gttggggaaa ctctggttac ttgtcagcat ggcgctcggc gtcattttcg tctggatgat
     9001 gacctgcgtg tatcaaatcg acacagtgcc aacctggtat aacggttaca ctacgctggc
     9061 tttcttcctg acagtattgt tgagcggtcc aatacttgct gccacaattc tgcgggcagc
     9121 ccgcgttaca tttaatacca caccatttgc catcattagc gttctggcgc tgatcgcttg
     9181 tgccggggtg attgtgctgc aaggtttgtc tctggcgtct attcactcat ccgtacaaca
     9241 agccagcgca ctggtaccag attacgcctc cttacaggtc tggcgtgtgg tattgttatg
     9301 cacaggtcta ggatgctggc tttgtccgct cattcgtcgc cgtgaacctc acgttgccgg
     9361 acttattctg gggctaatcc tcatacttgg cggtgagatg atcgggcgtg tgctctttta
     9421 tggcctgcac atgacggtag gaatggctat tgcaggttaa caaattggtg cggggcaacc
     9481 cgcacatttc aggatgttag gaatgaccca tttttcacag caagataatt tttctgtcgc
     9541 ggcgcgcgtg ttgggcgcgc tgttttatta cgctccagag agtgcggaag ccgcacctct
     9601 ggttgcggta ctcacccgtg acggctggga aactcagtgg cctctaccag aagcgtcatt
     9661 agcgccgctg gtgacggctt ttcagacaca gtgtgaagag actcacgccc aggcctggca
     9721 gcgtttgttt gtcggcccgt gggcactgcc gtctccgccg tggggatcgg tctggctgga
     9781 tcgcgaatct gtgctgtttg gcgattcaac attggcactt cgtcagtgga tgcgcgagaa
     9841 aggcattcag tttgaaatga agcaaaacga accggaagat cattttggtt cactgctgtt
     9901 gatggctgcg tggttggcgg agaatggtcg ccagacagaa tgcgaagaat tgctggcatg
     9961 gcaccttttt ccgtggtcaa cacgatttct tgacgttttt atcgaaaaag cagaacaccc
    10021 tttctaccgt gcactggggg aactggctcg cttaacgctg gcgcaatggc aatcacaact
    10081 gttgattcct gtcgcggtta aaccactctt tcgataggat caccgtaata ttgccggatg
    10141 gtggcgatta cgcgcaattc ggcaataata ggttacagtg tcacgttttt ttatctctta
    10201 aagcacgcac tgcttttgcg gctggcctct tttgccgcaa aatagtcgcc cgtgtttcat
    10261 tgcccatttc tgctcatgca tcatctacac atctatccgg atctgcgcac tatgttccgc
    10321 cgtctgctta tcgcaacagt cgtcggtatt cttgcggcct ttgccgttgc cgggtttcgt
    10381 catgcaatgc tgctactgga gtggttgttc ctcaataatg actcgggcag tctggtcaat
    10441 gcagcgacaa atctttcccc atggcgacgg ctgctaactc cggcgctcgg cggactggcg
    10501 gcgggtttgt tgctgatggg ctggcagaaa tttacccagc aacgccctca tgcgccgacc
    10561 gattacatgg aagcgttgca aaccgatggg cagtttgatt acgcagcaag cctggttaaa
    10621 tcgcttgcct ctctgctggt agtaaccagc ggcagtgcaa ttggtcgcga aggtgcgatg
    10681 attcttttag ctgcccttgc cgcctcctgt tttgcccaac gttttacgcc acgccaggag
    10741 tggaaattat ggatcgcctg tggagctgcg gcgggaatgg ctgcggccta tcgcgccccg
    10801 cttgctggca gtttatttat agccgaagtg ctgtttggca ctatgatgtt ggcctctctc
    10861 ggcccggtga ttatttccgc cgtcgtggcg ttgctggtta gcaatctgat taatcatagc
    10921 gacgcgttac tctacagcgt acaactctca gtgacggttc aggctcgtga ctatgcgctg
    10981 attatcagta caggtgtgct ggcaggtctg tgcggaccac tgttgttaac gttaatgaac
    11041 gcctgtcatc gtggatttgt aagtctcaaa cttgcgccgc cctggcaact ggcactgggc
    11101 gggttgatcg tgggtctgct ttccctgttc acacctgcag tgtggggcaa cggctatagc
    11161 accgtacaat cctttttaac cgccccgcca ctgttaatga tcattgccgg gatcttcctt
    11221 tgtaaactgt gtgccgtgct ggcgagtagc ggttccggcg cacccggtgg tgtctttaca
    11281 ccgacgctat ttatcggtct tgccattggc atgttgtatg gtcgtagcct gggattatgg
    11341 ttccctgatg gcgaagaaat tacgctttta ctcggattga ccgggatggc gacactgttg
    11401 gcggcaacca cgcacgcgcc gattatgtcg acgttgatga tatgtgaaat gaccggggag
    11461 tatcagctac tccccggttt attgattgcc tgcgtaattg cgtcggtaat ttcgcggacg
    11521 ttacaccgtg actctatcta ccgccagcac actgcgaagc atagctaagc ggatgtattg
    11581 ccccagttca cgctgttcgg cgcgcggcag ataaggcagt tcaccaatga gcggtgccgg
    11641 aagtttttta ccgagcacat caatgatttc cgcataatgc gccagtcctg gattgattcg
    11701 gttagccacc cagccaatga gcggcagccc gtcgttggca atcgcctgag ctgttagtag
    11761 tgcatggtta atgcaacctt cctgaatacc gacaaccatc aataccggca gttgttcctg
    11821 caccacccat tcagagagcg gacgcaaatc attcatcaga ctgcgccagc cgccagtccc
    11881 ttctaccacg acatgatcga ctttatcggt caggtttgcc aggccgtttg aaatgagggt
    11941 gtaattgatt gggcaactgt gcgccacgct actttcttct tcgcttaacg cgataggatt
    12001 aactgcttca taaggcagtt cgatggttga aacactctgc aacaccaggg catctttatt
    12061 acgcagccct tcgggtgtct ctttgctccc cttcgctacg ggtttatatc ccgcaaccgt
    12121 ttttccctgg gaggctaacg cttgtagcaa tgcgcgggaa accaccgttt tccctacaga
    12181 agtgtctgta ccggtaataa agaaacgctt cagcatcact aactccaccg ttatgcttca
    12241 caaatataaa ccaggaaaat aattaacctt gaaagtctaa gttatgcttt cctggcccaa
    12301 attgagatag cgcaaatttt ggtagaacag ttaaaaaatg ttaaccctgc aacagacgaa
    12361 tcaacaaaga accgttatac atcgcgtctt ttaccagcgc agcccctgcc atcgtaccct
    12421 ggttagaaaa ctgagtactc tcaacgctaa tgtgctgact atacgcagga agggcctgct
    12481 gacggatgct gtccgagatg accgggaaga ggatatctgc cgctttactt aacggtgagc
    12541 caatcagtat tttttgtggg ttaaataaat tcaccatgat ggcaagaatg cgccctacat
    12601 gcgcgcccac cccggtaatg atgtcttttg ccagtagatc gccgcgcaat gccgcctgac
    12661 acaatgaatc cacggttaac ggctgtccat gtaacatcga gctcatggat tgattgagac
    12721 gcagctgtgc cagctcaaga atactgtcca cactggcgat ggtttcgagg cagccgtgat
    12781 tcccgcaata acagcgtttc ccatacgggt cgacctgcgt gtgacctatt tccacgaggc
    12841 tactgctgcc tgcgtgtagc agatgaccat cggtaatgac gcccgccccc acgttgtgat
    12901 cgataaccac ctgaatcaca tcgcgcgccc cgcgtgaggc accaaacaag gcctcagcca
    12961 tcgtccatgc gctgatatca tgctgaatat aaaccggtac gccggtatgc tgctccagcg
    13021 cctcgccgag cggcatctct tttacatcct cgtagaacgg catgcgatgt acaataccat
    13081 tttccgtatc aataattccc ggcaaggtta tggcaatcga agtcagacgc tcaagttttt
    13141 tctggtggcg gataaaaaac tgatcgatat gggaaatgat acgatccagc aatggcgagt
    13201 catcttttaa cgccagttcc tgcgactcct ccactaccag tttgctgctc agatcgcgca
    13261 aagcaaggaa aatctccccg cgactaatgc gcagagaaag atagtgccag gcttcagttt
    13321 caaccaccag ccccactgcc ggacggccac ggttccccgc ttctttgatt tccagctctt
    13381 gcaccaggtg tgcttcgagc atctcacgga caattttagt gatactggca ggagccagct
    13441 gcgccagacg ggaaagatcg atacgcgaga ctggaccaag ctgatcaatc aggcgataaa
    13501 ccgcgcccgc gttggtctgc tttatttgat caatgtgccc aggctgattt tcagcaacca
    13561 ccgcatactc cctatatttt cgcgctccga aataatctgt aggctatggt gaagcacttc
    13621 aatacgtgtc gtcaaatttt tacttaggca tgtgattaac agcacatttt tcgggctttt
    13681 tcgctgaaat ttccctcacc tgagagcatt cagcagatga atacgaaagt tacgcgcagc
    13741 cggactttgt tcatggtgtt tcggccagac caaccacatt tcagaaaccg catcctcttc
    13801 agcaatcggc acccagcgca tttcgttgag ctgaacacgt ttaaatgacg caggcaaaat
    13861 tgaaacaccc agaccagcgg aaaccagacc gatgatggtc attgcctcac ccacctcctg
    13921 agtgatgacg ggcgtcaaat tgtaacgtcg catcagcccg agaatatcgt catacagccc
    13981 tgtccctacg tgcggatcaa aaaagacaaa gggttctttc gccagttcag ccagcgttac
    14041 actcgggtta tttgccaagg gatgatcgtg cgggatcatc gccataagcg gttcatggac
    14101 gatgactgcg tgttcaagcg tctccggtaa cgctgtgtta cgcagcaatc ccatatccag
    14161 cgttccttca atgagcggag cgatttgctc gcgggtgttc atttcgcggg tttgtaaatg
    14221 gacatcggga taatcacggc gaaacagcga taacgtatcg gacaccgccc gaataaaagg
    14281 agcggacgaa gtaaaaccaa tgcgcaactc ccccgcttca ccctgatgca gtctttcagc
    14341 gcgagcggca gcgtcatcca tcatagacag gatttgccga ctatctgcaa gaaactgttt
    14401 tcctgctgcc gtcagcaata cactgcgatt agttcgtgcc agcagtcggg caccaatttg
    14461 ttgctccagc gcctgaatct gctgacttag cggcggttgc gaaatattca ggcgggcagc
    14521 ggcgcgcccg aaatgcagct cttctgcaac agcaacaaag taacgcagat gacgaagttc
    14581 aatattcata tttaaaacat cttatttgag attattaata tattagacag aacaattcga
    14641 ttttcctaat ctattcataa gcctgaacca caggcatatt tagcaaggat ttcaagtgag
    14701 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
    14761 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
    14821 ctctgccgga ctggcaacct tcgcacttct ctattgtgtg caacctatcc ttccggtgct
    14881 ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttctacggc
    14941 gatgttggct atcggtttgc tgtttactgg cccgttatcc gatgccattg gtcgcaaacc
    15001 agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac
    15061 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtagc
    15121 agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat
    15181 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagcggtgt
    15241 cttcacggac tttttcaact ggcgaattgc gctggcggca atcggttgtt tcgcgctggc
    15301 ctcggcgtta atgttctgga aaatcctccc tgaatcacgc cattttcgcc cgacttcact
    15361 gcgccctaag acgttattca tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
    15421 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacgtcgg
    15481 ctatcggttg atgctctccc cctggcatgt cagccaggcc gtggttggct tattatcgct
    15541 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctatgg
    15601 gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg tttggtttat tgatgacctt
    15661 attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagccg gattcttcgc
    15721 agcccactca gtagccagca gctggatcgg cccccgcgca aaacgcgcta aaggccaggc
    15781 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
    15841 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
    15901 cattgctctg ctggtcggga cgcgtttgca tcatcgtctg cacgcctgaa aaaataagtc
    15961 cggactgagg taaatacccg tccggactta ttgcctgctc aaaccaacgt taatagccat
    16021 cctaaaatag acgaagcgcc agccaattcc cgcagcgcat ctagtgtcat caggattata
    16081 agtacccaaa taaacggatt cattttgctg tgtgtcattt attactgatg cgcagttatt
    16141 ctaccgcttt gtaagtagta aaatagttaa cacgatcaag actactatta ttggtagatg
    16201 aattttcctt aagtcacaat acgttattat caacgctgta atttattcag cgtttgtaca
    16261 tatcgttaca cgctgaaacc aaccactcac ggaagtctgc cattctcagg gatatagtta
    16321 tttcaacggc cccgcagtgg ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa
    16381 aagtattagc tctggttgtt gccgctgcta tgggtctgtc ttctgctgcc tttgctgcag
    16441 agactgcgac cacacctgct ccgactgcga cgaccaccaa agcagcgcct gcgaaaacca
    16501 cacatcataa aaaacagcat aaagcagcac ctgcccagaa agcgcaggcg gctaaaaagc
    16561 atcataaaaa tgccaaagct gaacagaaag ctcctgaaca aaaagcgcag gcagcgaaga
    16621 aacacgccaa gaaacacagc catcagcaac cggcaaaacc tgctgcacaa cccgcagcgt
    16681 aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg aaaattacgg tgctaaacgg
    16741 gtaacgttta gcaccgcctt tagcggaggg tagtatggtg ggccgttatc gctttgagtt
    16801 cattcttatc atccttattt tatgcgcact gattgccgcc cgtttttatc tttcctgatc
    16861 gtagttatct gattttactc ccactttcat cccgtcccgt ctatagtatt tatgagggtt
    16921 tgcttttaat aatcataatt acccaccaga gtgtgatatg cgtagaacca ttgctgtagt
    16981 gttgggtgca attagtttga cgtctgcttt tgtgtttgca gataaaccag acgttgccaa
    17041 atcggcaaac gatgaggtca gcaccctgtt ttttggtcat gatgatcgtg tgccagtgaa
    17101 tgacacgacc caatcaccgt gggatgcggt tggccaactg gaaacggcca gcggcaattt
    17161 atgtacagcg acgctgattg cacccaatct ggcattaacg gcaggacact gtttattgac
    17221 gccgccaaag ggtaaagccg ataaagcggt ggcgctgcgt tttgtgtcaa ataaaggtct
    17281 ttggcgctat gagatccacg acatagaagg ccgcgttgac ccgacactgg gaaagcggtt
    17341 aaaagcagat ggggatggtt ggattgttcc tcccgcagct gcgccgtggg acttcggttt
    17401 gattgtgcta cgtaatcccc cttctggcat tacaccattg ccgttatttg agggagataa
    17461 agccgcgctt accgccgcat taaaagcggc aggtcgtaaa gtgactcagg cgggataccc
    17521 tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt gaagtgactg gctgggcgca
    17581 aacgtcggtg atgtcgcatc agtgcgatac cttgccgggt gacagcggtt cgcctctgat
    17641 gttgcatacc gatgacggct ggcaattaat tggggtgcaa agttcggctc ctgccgcgaa
    17701 agatcgctgg cgcgccgata accgggccat ttctgttacc ggttttcgcg acaagctgga
    17761 tcaactgtcg caaaaataat gttcaaacgc tgcccgacag cgcgggcagc atgttcatca
    17821 ggcaagtttc accatgatca ttccagccaa cagcagcacc aggccaatcc agcctttacg
    17881 atttaaccgt tgaccaaaca agatccaacc tgcggctaac gtggcggcaa taccaaaccc
    17941 gccccacaat gcataagcga cagacaagtc gatcccttta acggcctgag aaagcgcact
    18001 aaaggcagcc agcaccgccg ccagagagag caagccaaat attttgcgac gaaagccgtc
    18061 agaaaatttc aaaaagacgt tagcaacgat ttccagcacg attgccaatg ccagccaggc
    18121 ggcgtgaacc cattcaaact gcgccatggt tcacctccag ttcaggttta cgcgctttac
    18181 gggtacctga tttgatcaac acaatcccgg cgaccagggt ggttaacccg gcaattttca
    18241 tcagagataa actttcgtcg aataacaaaa cgctaaacaa ggtaataaat aaaataccga
    18301 taccttccca cagcgcataa gctacgccta aggcgatctt tttaacggcg aaagagagaa
    18361 atatatacga cagagaaatc atcaccagca ttaaaataaa gccgccattt ccctcactga
    18421 cgctcgccca tttcatggac agcgtaccgg taatttctgt agcaatagcc agacctaata
    18481 aaatccaata aatatacatt gtccttctcc tgcaagagaa ttattttaat tttcgcttaa
    18541 ttcagcgaaa ccaaagtaaa gtggtcaagc ccgcgcttag cgcaagtttt ggcagaagaa
    18601 aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac
    18661 gaagatgggt aaatgtagaa aataacgtcc tgaacaaatt gtccataata ttacaattat
    18721 ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg aacacgcctg
    18781 atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc tgagcggttt
    18841 gattttcgtt atgatgacgg agcgaaaaag acattattat tagcaaagga agaaaaaacg
    18901 gggacaagca tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt cattatgttg
    18961 ggaatgctgg tcattattct ctgcggtatc cgttttgccg ccgagatcat cgtgccgttt
    19021 attctcgcat tatttattgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg
    19081 cgtgtgccgc gtgtactggc agtgtcaatt ttgatgacca tcatcgtgat ggcgatggtg
    19141 ttgctgttag cttatctggg ttccgcgctc aatgagttga cgcggacgtt acagcaatat
    19201 cgcaactcta ttatgacgcc actgaaagcg attgaaccgt tgttgcaacg cgtagggatt
    19261 gatgtctcag ttgaccaact ggcgcattac attgatccga acgcggcgat gacgttgctc
    19321 accaacttat tgacgcagtt atctaatgcc atgtcatcaa tctttttatt gctgctgacg
    19381 gtgctgttta tgctgctcga agtgccacaa ttgcccggaa aatttcagca aatgatggcg
    19441 cgtccggttg aagggatggc ggcgattcag cgtgcgattg acagtgtgtc tcattatctg
    19501 gtgctgaaaa cagccatcag catcatcacc ggcctggtcg catgggcgat gctcgccgca
    19561 ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatcccg
    19621 aatattggtt cagttctcgc ggcaatcccc cctattgctc aggtactggt gtttaatggc
    19681 ttctacgaag cgttgctggt gctggcggga tatctgctga ttaatctggt cttcggcaat
    19741 attctggagc cgcgcatcat gggacgtggg ctggggcttt cgacattggt ggtatttttg
    19801 tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgttg
    19861 acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat cgccgttctg
    19921 ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt acagagcttt
    19981 caggattgca tccacgctgg ctttggcgtc accaaacagc atgtgggtgt tttccttgaa
    20041 gaacagcggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac
    20101 gttctgcgct ttccacactt ccagcacagg cataccagca ataggactct tcggatcatc
    20161 ctgcgccgcc gggttaaccg tatcgttagc accaatcacc agtacggtat cggtatcagc
    20221 aaagtcatcg ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc
    20281 cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc gggtggatac cgaaacgcac
    20341 gttgatacca cgagcgcgca gtttctcggt aatttcagcg acaggatatt gcgcctgcgc
    20401 gactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca gcagttccgc
    20461 tgtctcttct gcggtgattt cgcggtgctc gcccacttcc tgatcatcgc cagtagaaga
    20521 gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca
    20581 cataatgtaa gaaaggatag cgcccgaaga accgaccagc gcaccggtca caatcagcag
    20641 atcgttgctg agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat
    20701 cgacaccacc actggcatat ctgcaccacc gatggaggcg actaaatgcc agccgaatac
    20761 cagcgcaatg gcggtcatta tcagcaatgc cagcacttgc aggccgacgc tgtccgtgcg
    20821 aacaaataca atcagcagca ggaaggaaac gaccagagcc gccaggttca ttttgtgacg
    20881 gtttggcagc atcaacggtt tagacgaaat cttgccacac agtttgccga acgccaccac
    20941 cgaacccgtg aacgttaccg ccccgatgaa gataccgagg aacacttccg tcaggtgaat
    21001 attgaccaga atcggtgcca ttcccgcgtc atgatgcaga tagctgttaa agccaaccag
    21061 cactgccgcc agacccacga agctgtgcag gatcgccacc agttctggca tttcggtcat
    21121 ttcaactttc ttcgccagcc ggataccaat tgccccacca atgaccatcg ccagcaagat
    21181 ccagccaaca ttacccgtat ccggtcccaa aatggttgcg attaacgcaa tcgccatccc
    21241 ggcgataccg aagttgttac cctggcgaga cgtttcatgt ttcgaaagac cggccagact
    21301 gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg
    21361 ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga
    21421 aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct
    21481 ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca
    21541 tcaacggtgt atgcagcgcg tgcgatacat tccacaccac gtaataaccg acaacacagg
    21601 ccagcgcaaa aacggtgaag tgcccaagaa actctttcgg cgcaacgctt gccaaccagc
    21661 cgaaaagaat gattgccagc gccatcaacg cgtatttacg ccacggtgag caggcacatt
    21721 tttcctcggt tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga gctgatacct
    21781 gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacggtcacg ccgcgaatca
    21841 ccacatcatc aaaatcaaca gtgatattgc cgtctttctc tttgcacaac agtttcagca
    21901 gattaacgag gttagtaccg taaagctgtg aggattgcgt cggcagacgg cccggaagat
    21961 cggtataacc aatcactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt
    22021 attcacagtt gccgccgttt tgggctgcca ggtcgacaat cacactaccc gccttcatgg
    22081 agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg
    22141 tgacaatgat atcgacctct tttgcctggg cggtaaagag ttccatttcc gctttgatga
    22201 acgcgtccga catcactttg gcatagccat cgccgctgcc cgcttcctct ttaaaatcca
    22261 gctcgaggaa ttccgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga
    22321 atgcacgcac aatcgcgccg agactgtttg cagcgccaat ggcggccaga cccgcaacac
    22381 ccgcaccaat caccatcact tttgccggtg gcactttccc ggccgcagta atttgcccgg
    22441 taaagaagcg cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatattcg
    22501 ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gagtccatcg
    22561 ccatcacggt cacgttacgt tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc
    22621 agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg
    22681 cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca atttcagcgc
    22741 ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg
    22801 cgacggtaaa acccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg
    22861 tttcattggt taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt
    22921 ttattgatga tggtttgcct gtgtcaggag ccacacaagc cgctcatgta cgagctaaat
    22981 gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg
    23041 tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc
    23101 gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt cttcggcgtt
    23161 cgcataaaac gagacatcgc cccggcacga atcactactt aacattaaat taacttatac
    23221 aattcagttg cttcagtagt attgatgctg atacggctgt tttttaagca tagacggcca
    23281 tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc agacagttac
    23341 accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt
    23401 gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc
    23461 tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag
    23521 cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg
    23581 tgaaagccgt ttctcgtcgc gctgataaag aaggtgccgc ctctttttat gttgtcgaca
    23641 cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg
    23701 ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag
    23761 atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc
    23821 agccagaagt caatgatgcc atcaccaaag cggcaaaagc gaaaggtgcc tactctttct
    23881 acatcgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct
    23941 ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg
    24001 aagcaggacg tgcagctctg gctgccggtg gcgaagctgc gaagaaagtt gagatcccgg
    24061 gtgttgcgac taccgcatca ccaagttctg aagtcggtcg cttctttgaa acccagtcat
    24121 caaaaggcgg gcgttacact gtcacgctcc cagacggcac taaagtcgaa gaactgaaca
    24181 aagcgaccgc agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca
    24241 acatgactga agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt
    24301 accacatcac ccgccagtgg caagaacgtg gtaataacct gaccgtcagc gcagatctgt
    24361 ataaataact attcagttac tcctataagg cggcttgatg ccgcctttcg catttttagt
    24421 cgcaaatttc cccgcgacca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca
    24481 gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttgt cacgttttaa
    24541 tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc tggtattaag
    24601 ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag ttgccagccc
    24661 ggcagcactg ctcatcggct ggagtattac tggtgctggt attttattac tgacctttgc
    24721 catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta cctatgcccg
    24781 cgaaggattt ggtgagctaa tcggcttttg ttccgcatgg ggatactggc tgtgcgcggt
    24841 catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt ttacggacac
    24901 gccggaattg cgcctgtttg gcgatggtaa tacctggcaa tcgatcgtcg gtgcatcggc
    24961 tttattgtgg attgttcact ttttgatcct gcgcggagtg caaaccgctg ccagcattaa
    25021 cctggtggcg acactggcaa aattgttgcc gctgggtctg tttgttgtgc tggcgatgat
    25081 gatgttcaaa ctggatacct tcaagctcga cttcaccgga cttgcacttg gcgtacccgt
    25141 ttgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg gtgtggaagg
    25201 tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag cgacactgct
    25261 ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc tgggcgtggt
    25321 ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggactga tggtggaaat
    25381 gatgggaccg tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg tttgcggtgc
    25441 gtacttaagc tggaccatca tggcggcaga agttccgttc ctggcagcca ctcataaagc
    25501 attcccgcgc atttttgcgc gccagaacgc acaagctgcg ccatccgctt cactgtggct
    25561 gaccaatatc tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt ccgattacaa
    25621 cacattgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg tcggcgcgtt
    25681 cttgctgaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg cctgcattta
    25741 tggcttatgg ttattgtacg cctctggtcc aatgcacttg ctgttatccg ttgttctgta
    25801 tgcaccgggg ctgctggttt tcctgtacgc gcgtaaaacg catacccatg ataatgtact
    25861 gaatcgtcag gaaatggtgc tgattggcat gctgttaatt gcctcagttc cggcgacctg
    25921 gatgctggtg ggataacgtt catcccatcg ttggatagca aaggagataa cgatgggtaa
    25981 agcccaaccc ttgccaatat taattactgg cggaggtcgt cgcatcggcc tcgccctcgc
    26041 ctggcatttc attaatcaaa agcaaccggt gattatcagc tatcggacgc actatcccgc
    26101 cattgatgga ctgattaacg caggtgcgca gtgtattcag gctgattttt cgaccaacga
    26161 cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggcctgc gtgctatttt
    26221 gcataacgcc agcgcgtgga tggcagaaaa accaggtaca ccactgaccg acgttctggc
    26281 ttgcatgatg cagatccacg ttaatacccc atacctactc aaccatgcac tggaaagatt
    26341 actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt atgtggtgga
    26401 gcgcggtagc gacaaacata ttgcctatgc tgcaagcaaa gcggcactgg ataatatgac
    26461 ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg cgccatcgct
    26521 gatcctgttt aatgagcatg atgatgccga ataccgacaa caggcgctga ataaatcact
    26581 gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact tacttaccag
    26641 ctgctttgtc accggacgca gtttccctct tgatggcggt cgtcatctgc gttaatgcag
    26701 ctttatccag catataatca acacccatgc gcttatcccc cagcatgcaa cagaaccaac
    26761 aaaagcggcg ggcaagcgca tcattccggt gaaataccac agcgacgcca ggtaaacaaa
    26821 atagggaata atcgaccaca tgctaaaaat gatggttgcg cgtaaggctt caatgccgcg
    26881 ttcgctggca acaatataat gcgcgatgag cgcaaaggtc gggaaaagtg gtatcagccc
    26941 ggcgatataa taatttttcg tttttgctaa aacaccaatc aacagcacta ccagcgcacc
    27001 gagcgcggct ttgattacca gccccatctt tttaccttaa cacttccata acaagttatc
    27061 agtagaatac ctgatgaaac cttgtttaga aacgattgat agtaagtaaa aacagcgcgg
    27121 tgtattgtga cgtttttata tctaccgtga atgttatgaa cactatcgta tttgtggaag
    27181 atgatgcgga agtcggttca ctgattgccg cgtacctggc aaaacatgat atgcaggtta
    27241 ccgtggaacc gcgcggcgac cgggccgaag aaactattct gcaagaaaat ccggatttgg
    27301 tgttactcga catcatgcta ccaggcaagg acggcatgac catttgtcgt gatttacgcg
    27361 caaagtggtc cggaccgatt gttcttctaa cctctctcga tagcgatatg aaccacatcc
    27421 tggcactgga aatgggtgcc tgcgactata ttctcaaaac gacgccccct gctgttttgt
    27481 tagcgcgttt acgtttgcat ttgcgtcaga atgagcaagc cacactgagc aaaggtcttc
    27541 aggaaacgtc tctgactccc tacaaagccc tgcatttcgg cacgttgacc atcgatccta
    27601 tcaaccgcgt agtcaccctg gctaacactg aaatctcgct ctcgacagct gatttcgaat
    27661 tattgtggga attagctacc catgctgggc aaatcatgga ccgcgatgca ttgctgaaaa
    27721 atttacgcgg cgtcagttat gacggactgg atcgtagcgt ggacgtggct atttcgcggt
    27781 taagaaaaaa actgctcgat aacgccgcag aaccttatcg cattaaaact gttcgtaaca
    27841 aaggctatct ttttgcgcct catgcatggg aataagcgat gaaaaaactg tttatccagt
    27901 tttacctgtt attgtttgtc tgcttccttg tgatgtctct gctggttggg ctggtgtaca
    27961 aatttaccgc cgaacgcgcg ggcaaacagt cgctggatga tttgatgaac agttcgctgt
    28021 atctgatgcg cagcgaattg cgtgagatcc ccccacacga ttggggtaag acgctgaagg
    28081 agatggattt aaatctctct ttcgatctgc gagtcgagcc actgagtaaa taccatcttg
    28141 atgatatttc catgcaccgg ctgcgtggcg gcgaaattgt cgccctggac gatcagtaca
    28201 cttttttgca acgtatcccg cgcagccact acgtgctggc agttggtcct gttccttatc
    28261 tttattacct ccatcagatg cgattactgg atatcgccct gatcgctttt attgccattt
    28321 ccctcgcctt tccggtgttt atttggatgc gtccgcactg gcaggatatg ttaaaactgg
    28381 aagcagcggc gcaacgattt ggcgatgggc atctcaatga acgtatccac tttgatgaag
    28441 gttcgagctt tgaacgactt ggcgtcgcat ttaaccagat ggcggacaat atcaacgcct
    28501 tgattgccag caaaaaacag cttattgacg gtatcgctca cgaactgcga acaccgttag
    28561 tgcgcctgcg ttatcgactg gagatgagcg ataacctgag cgccgtcgaa tcccaggcgt
    28621 tgaatcgcga tatcagtcaa cttgaagctt taattgaaga gctgctgact tatgcccgac
    28681 tcgatcgccc acaaaatgag cttcatctta gcgaaccaga cctgccgttg tggctgtcaa
    28741 cgcatctggc agatattcag gcagtaacgc ccgataaaac ggtacggata aaaacgctcg
    28801 cgcaaggcca ttatgctgcg ttggatatgc gcttaatgga gcgcgtgctg gataatttgc
    28861 tcaataacgc cctgcgctac tgtcactcaa cggttgaaac cagcctgcta ctgtcgggga
    28921 atagagcgac attaattgtt gaggatgatg gcccagggat tgcccctgaa aaccgcgaac
    28981 atatctttga accttttgtt cgcctcgatc ccagccggga tcgctcaacc ggcggctgcg
    29041 ggctggggct ggcaattgtc cactctatag cactggcaat gggcggtacg gttaattgtg
    29101 acaccagcga actgggtggt gcccgcttct cgtttagctg gccgttatgg cataacatcc
    29161 cgcaatttac ctctgcttga cattacgcgc acggtggtca agtcaccacc actgtgctat
    29221 aaaataagga tgttgtaact aaagtggtta atattatggc gcgttacgat ctcgtagacc
    29281 gactcaacac tacctttcgc cagatggaac aagagctggc tgcatttgcc gctgagctag
    29341 aacagcacaa attgctggtt gcccgcgtgt tctctttgcc ggaggtaaaa aaagaggatg
    29401 agcataatcc gcttaatcgt attgaggtaa agcaacatct cggcaacgac gcgcagtcgc
    29461 tggcgttacg tcatttccgc catttattta ttcaacaaca gtccgaaaat cgcagcagca
    29521 agaccgctgt tcgtctacct ggcgtgttgt gttaccaggt cgataacctt tcgcaagcag
    29581 cgttggtcag tcatattcag cacatcaatc aactcaagac tacgttcgag catatcgtca
    29641 cggttgagtc agaactcccc acagcggcgc gttttgaatg ggtgcatcgt catttgccgg
    29701 ggctgatcac ccttaatgct taccgaacgc tcactgttct gcacgatccc gccactttac
    29761 gttttggctg ggccaataaa catatcatta agaatttgca tcgtgatgaa gtcctggcac
    29821 agctggaaaa aagcctgaaa tcaccgcgca gtgtcgcacc gtggacgcgc gaggagtggc
    29881 aaagaaaact ggagagagag tatcaggata ttgctaccct gccacaggac gcgaagttaa
    29941 aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt ctggtacaag ggagatcaaa
    30001 aacaagtaca acacgcttgc cctacaccgc ttattgcgct gattaatcgg gataatggcg
    30061 cgggcgtgcc ggacgttggg gagttgttaa attacgatgc cgacaatgtg caacaccgtt
    30121 ataaaccaca ggcgcagccg cttcgtttga tcattccacg gctgcacctg tatgttgcag
    30181 attaaccccc ggctttcata ctgccgacca tctgttctgg ccgtacccag ctgtcaaact
    30241 cggcttcgct aagatacccc agcgcaaggg ccgcagcttt taaggtcagc ccttctttat
    30301 gcgctttttt ggcgatctcg gcggctttgt cataaccaat gtgggtgtta agcgcagtca
    30361 ccagcatcag cgattcattg agtaattgat tgattcgctc acgattcggt tcaatgccca
    30421 ctgcgcagtg tttgttaaaa ctttccatgc catctgccag caagcgcacc gattgcagga
    30481 aattatggat caccatcgga cggaagacgt tcagttcaaa gttaccggaa gcgccaccca
    30541 tgttgatcgc cacatcgttc cccatcacct gacagcagag catggttaat gcttcacact
    30601 gcgtcgggtt cactttcccc ggcatgattg agctacccgg ctcattttcc gggattgaga
    30661 tttcaccaat tccgcagcgc gggccagagg ccagccagcg gacatcattg gcaattttca
    30721 tcagtgacgc agccaaccct ttcaacgcgc cgtgcgcctg aaccagggca tcacaggtcg
    30781 ccagcgcttc aaatttgttc ggcgcggtaa caaacggtgc acaagtaatg actgccagtt
    30841 catctgccac gcgacgcgca tactccggat gggtatttag tccagtaccc accgctgtac
    30901 cgccaagagc cagttccgct acatgcggca ggctgtattc gatatgtttc agattatgct
    30961 cgagcatcgc tacccagccg gaaatctcct gccccagcgt taacggcgtg gcgtcctgca
    31021 agtgggtacg accgattttg acgatatcag caaatacgcg ggatttttca ctcagcgtct
    31081 gggtcagggt tttaagttgt ggaatgagtt gcttgcgcag cgccagtagt gccgcaacgt
    31141 gcatcgccgt cggaaagaca tcgttggaac tttggctttt gttcacgtcg tcgttagggt
    31201 gaactttacg ttccatcccg cgcacgccac cgagtaattc actggcccgg ttagccagca
    31261 cttcgttcat gttcatgtta ctttgcgtgc cggagccggt ctgccagata gccagcggga
    31321 attcgtcgtc atgctgtcct gccagtactt catccgccgc ctgccgaatg gcgctcgctt
    31381 tctcttcaga caacaagcct aaatcttcat taacttttgc cgctgcgcgc ttggttagcg
    31441 ccagcgcatg aatcagtgag gtgggcattt tctccgtcga aatgcggaaa tgctccagcg
    31501 agcgctgagt ttgtgcgccc cacagcttat ctgccgggac atcaatcgct cccatcgaat
    31561 ctttttcgct gcgtactgta ttcatggcct gctcctcacc tgattaattt tttctttctg
    31621 ttttgctttc gttaagcaac ttttagctta ctcattattt accatttgat aacaaatgtt
    31681 tggtctttcg tgccatgtaa aaaaaccgcc ccgaagggcg gctctgttta tttcacacag
    31741 cgggtgcact gtgtgagttg tatctgctgg aagaagtcat ttcctttatc atccacaagg
    31801 ataaatgccg ggaaatcttc cacttcaatt ttccagatgg cttccattcc cagttccgga
    31861 tattcaacac attccaggct cttaatgctt ccctgcgcca atacagctgc cggaccaccg
    31921 atactgccaa ggtagaagcc gccgtgtttt ttacaggcat ccgtcacctg ctggctgcgg
    31981 ttgcctttcg ccagcatgat catacttccg ccctgcgctt gcagttgatc gacataagaa
    32041 tccatccgtc cggcggtcgt tgggccaaga gaaccggagg cataaccttc cggcgttttg
    32101 gccggacccg cgtagtaaat cggatgatct ttaatatact gcggcagccc ttcgccgtta
    32161 tccatccgct ctttcagttt ggcgtgagca atatcacgac caacgataat cgtgccattc
    32221 agcgataagc gtgtagaaac gggatactgc gacaactgtg cgaggatctc tttcatcgga
    32281 cggttaaggt caacgcgcac cgcttcgcct tctcccgctt tacgcagctc ttccgggata
    32341 tatttgcctg gattatgttc cagtttttcg atccagatcc cctgacggtt gatcttcgct
    32401 ttgatattac ggtcagcaga gcaggagacg cccataccga ccgggcagga tgcgccgtga
    32461 cgtggcaggc gaatcacgcg gatgtcgtga gcgaagtatt taccaccaaa ctgcgcaccc
    32521 agaccaagat tttgcgcttc gatcagcaat tctttttcca gttccacatc gcggaacgcc
    32581 tgaccgtgct cattcccttc cgttggcagt tcgtcatagt atttagcgga agccagtttc
    32641 accgttttaa ggttcgtttc cgcagaagtt ccaccaatta cgaacgcaat atgatacgga
    32701 ggacaggccg ccgtacccag cgtgcgcatc ttctcaacca ggtaattttt cagtttcccc
    32761 ggcgttagta acgctttggt ttcctgatag agatacgtct tgtttgccga accaccacct
    32821 ttggcgatac agaggaattt gtactcgtcg ccatcaacgg cataaagatc gatctgcgct
    32881 ggtagattgg tgccggtatt cacctcttta tacatatcca gcggcgcgtt ttgcgagtag
    32941 cgcagattat cttcgatata agtgttatag acaccgcgtg ccagcgccgc ttcatcaccg
    33001 ccaccggtcc atacacgctg cccttttttg ccaacaataa tcgcggtgcc ggtatcctga
    33061 caggttggca gaacgccttt cgccgcgatg tcggagttgc gcaggaattg cagcgccaca
    33121 tatttatcat tttcgctggc ttccgggtca cgcagaatgt cggccacctg ttgttggtga
    33181 gccggacgca gcataaatga cgcatcatga aacgcctgac gcgccaacaa agttaacgct
    33241 tcgggtgcga ctttcaaaat ctcctgccct tcaaattcag atacgctaac gtgttcgctg
    33301 gttagcaggt aatactcagt atcatctttt ttgagtggaa aaggagcctg ataatgaaag
    33361 ggtttgtttg acattgttct ctcacttact gcctggtttg gttatgctct gggcgggtgt
    33421 tccgttgccc tgttaaaagc gagtaacaat atcctacaca cttttttaac aaaaactgag
    33481 actagtacga ctttttgcgg ctccaggtta cttcccgtag gattcttgct ttaatagcgg
    33541 gattaatttc cacattaaaa cagggattga tcatgcaaaa actcattaac tcagtgcaaa
    33601 actatgcctg gggcagcaaa acggcgttga ctgaacttta tggtatggaa aatccgtcca
    33661 gccagccgat ggccgagctg tggatgggcg cacatccaaa aagcagttca cgagtgcaga
    33721 atgccgccgg agatatcgtt tcactgcgtg atgtgattga gagtaataaa tcgactctgc
    33781 tcggagaggc cgttgccaaa cgctttggcg aactgccttt cctgttcaaa gtattatgcg
    33841 cagcacagcc gctctccatt caggttcatc caaacaaacg caattctgaa atcggttttg
    33901 ccaaagaaaa tgccgcaggt attccgatgg atgccgccga gcgtaactat aaagatccta
    33961 accacaagcc ggagctggtt tttgcgctga cgcctttcct tgcgatgaac gcgtttcgtg
    34021 aattttccga gattgtctcc ctgctccagc cagtcgcagg tgcacatccg gcgattgctc
    34081 actttttaca acagcctgat gccgaacgtt taagcgaact gttcgccagc ctgttgaata
    34141 tgcagggtga agaaaaatcc cgcgcgctgg cgattttaaa atcggccctc gacagccagc
    34201 agggtgaacc atggcaaacg attcgtttaa tttctgaatg ttacccggaa gacagcggcc
    34261 tgttctcccc gctgttgctg aatgtggtga aattgaatcc tggcgaagcg atgttcctgt
    34321 tcgctgaaac accgcacgct tacctgcaag gcgtggcgct ggaagtgatg gcgaactccg
    34381 ataacgtgct gcgtgcaggt ctgacgccta aatacattga tattccggaa ctggttgcca
    34441 acgtgaagtt cgaagccaaa ccggctaacc agttgttgac ccagccggta aaacaaggtg
    34501 cagaactgga cttcccgatt cctgtggatg attttgcctt ctcgttgcat gaccttagtg
    34561 ataaagaaac caccatcagc cagcagagtg ccgccatttt gttctgcgtc gaaggcgatg
    34621 caacgctgtg taaaggttct cagcagttac agcttaaacc gggtgaatca gcgtttattg
    34681 ccgccaacga atcaccggtg actgtcagag gccacggccg tttagcgcgt gtttacaaca
    34741 agctgtaaga gcttactgaa aaaattaaca tctcttgcta agcttattaa aggcttataa
    34801 caccttcagg cggccagtcc gcctgatttc attttatgga taatcattat gaataaatcg
    34861 ctggtagcgg taggcgtcat tgttgcgcta ggcgtagtct ggacaggcgg cgcatggtat
    34921 acaggcaaga agattgaaac ccatctcgaa gacatggtcg cgcaggcgaa cgcgcaactc
    34981 aaactgaccg ctcctgaatc caacctggaa gtgagttatc aaaactatca tcgcggcgta
    35041 ttcagcagtc agctgcaact gttggtgaaa cccattgccg gaaaagtaaa tccgtggatt
    35101 aaaagcggtc agagcgtcat cttcaacgaa tcggttgatc atggtccctt cccgcttgcc
    35161 cagcttaaaa aactgaatct gatcccgtcg atggcatcaa ttcaaaccac gctggttaat
    35221 aacgaagtaa gcaaaccact gtttgatatg gcaaaaggtg aaacgccttt tgagattaac
    35281 tcccgcattg gttacagcgg agattccagt tccgacattt cgctcaagcc actgaattac
    35341 gagcaaaagg atgaaaaagt cgcctttagc ggcggcgagt tccagttaaa tgcagacaga
    35401 gacggcaaag ctatctccct ttccggggag gcgcaaagtg gtcggataga cgctgttaac
    35461 gaatacaacc agaaagtaca gttgaccttt aataatctga aaaccgacgg ttccagcacg
    35521 ctggcaagtt ttggtgagcg cgtaggaaac caaaaactgt cactggaaaa aatgaccatt
    35581 tcagtggaag gcaaagaact ggcactgctg gaaggcatgg agatcagcgg taaatcggat
    35641 ctggtcaatg atggtaaaac gatcaatagc caactggatt actcgctaaa cagcctgaag
    35701 gtacagaatc aggatctggg cagcggcaag ctgactttaa aagtcggcca aattgatggc
    35761 gaagcctggc atcagtttag ccagcaatat aacgcgcaaa ctcaggcgct gctggcacag
    35821 ccagaaattg ccaacaatcc cgaactttat caggaaaaag tgacggaagc cttctttagc
    35881 gccctgccgc tgatgttgaa aggcgatccg gtgattacta tcgcgccgct aagctggaaa
    35941 aacagtcagg gtgaaagtgc gctgaatctg tcgctgttcc tgaaagatcc ggcaacgact
    36001 aaagaagcgc cgcaaacgct ggcgcaggaa gtagaccgtt cggttaaatc tctggatgcg
    36061 aaactgacca ttccggtgga tatggcaact gagttgatga ctcaggtagc gaagctggaa
    36121 ggttatcagg aagatcaagc gaaaaaactg gcgaaacagc aagttgaagg tgcatcagca
    36181 atggggcaga tgttccgtct gaccaccttg caggacaata ccatcaccac cagcctgcaa
    36241 tatgctaacg gtcagatcac gttaaacggg cagaaaatgc cactggaaga ttttgttggg
    36301 atgtttgcga tgccggcatt aaacgttccg gctgtacccg ctattccgca gcagtaattc
    36361 acagccaaaa tgccggagat ttttctctcc ggcatttttt cttacttcag cataaagtca
    36421 tactgaatac tcattttcac atcccagtaa ttcaggcgat cgcccagcgc actgccatcg
    36481 cgcttttccc gccctttatc ggcagcaaga aagaaattca tcccttgtaa cggcccttcg
    36541 ctgacccgat agttcatacc acctaagata cccgcgcctt caatccgcac atctcgttta
    36601 ctggattcat agccgcgagt cacaatcggt gcaaatgtca gcgtaaattg cggcgttaaa
    36661 cgatagttga cacccaattg ccaggactgc attccttcac ggttacgatc aagacttaat
    36721 gaaaagggat agccaatgtc ggtatccacc acggcaccat tcattgcatg gcgcaaataa
    36781 ccaccactgc caaatacgct tccccacggc gcattccagg tcaattgtcc actaaccaac
    36841 gccgtttcat tgggctggct ggtattacga cttaaacctt caagttgcgc gtaaaagccg
    36901 agcaactccc ctttcaacgt ataattcggg tcaagcggta tgcttttctc gagaatactt
    36961 tgtatctgat tggaaacatc tttctgtacc gatgccccca gagaatagcg actggtatca
    37021 tcatgccaac tggcggctaa aaaagaacgt gcgcgatgtt tatcgtattt tcccgtctgg
    37081 ctgttataca ccccttcatc acgaataccg gttgttgaat cggtccataa aaatactcgt
    37141 gaccgccaca ttccccacag atcaatattt ggcgttaaag cccagtaacc taatgctcct
    37201 tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca ttaacggaat acgataaggt
    37261 aagctaccat aactataatc cgtcccgaag cgacctaatt ttagcaggct attttcactg
    37321 ggttttattt ttactgcccc cagggcaaag ccaaaacttt tatcaccatc aagataccac
    37381 cgggtactca tatcagcacg agcacctaat ttataaacat aatacgcccc gccttcaacg
    37441 ccaataatat tggaataata accactattg aaatcgagca atcctccctg tacccatgcg
    37501 taaatatctc ttcccgtgcc agcactgggc ttatcagccc tgcgtaattc gtttttcagt
    37561 ctgatgcgta gtcccgcttc atcgtcagcc ggttgcgccg cgtaagcaga ggtaaggcca
    37621 gaggcagccg tcaggcaaat aacggccatg gccactattt ttctcatagt tgcacctttg
    37681 atgttctgat tatttttatt gaatattaac tagtgatatc gttgattaat tgctgctgaa
    37741 cttttttacg attatcaatt tcaacaacga tttctttgaa ttttttatcc gtgagcggat
    37801 aaaaccagat aatgacgaat gccagtagca taaatccgca aggtactaag gcaattgatg
    37861 tgcggatgcc cataataact tccggcgttt gcgcctgatt ggcgatatat ccgcttaatc
    37921 ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc gcatttacgg gtaaatgaga
    37981 atagtgaata ggtgagccct tcaattcgca cgccggtcag gtattcaccg tattctacgg
    38041 tatcagcttc cagtgcccac atcacggtca tggtaacgcc ctggccaatt gatgcgatgg
    38101 ccaacgcaac caacgccacc ggcagtgacc agacggaaac ccagaagaac agcagataac
    38161 cgcaggttcc cagcaaagcg ccaatcagga aggtattctt tttaccgatc ctcgcgacca
    38221 tccccggcac cagcggtgcc gatgccacag taccgaccag gttttgcacc agtaccagca
    38281 cagtgaacag cccggtatca tttaacacat agcgcacgta gaacaacgac gaggcgctga
    38341 ccgcgaaggt cgaaatcagc acacacagcg caccgatgca caacataaac agcgggcgat
    38401 tccgtttcag ggtttgcaga ctgatcttca atgacggctg cgccacgata cgtaccacat
    38461 tctcacgcgt cgatttgaag cagatgaagt aaagcaccat tccggcaatc gccagcacaa
    38521 ttgtccagaa atgatatacc gacaccatct cttccgggct ggagttctta atgctcggtc
    38581 ctatcagaaa tgccaggcag acaaaagtca atgaagcggc aatcccgcga gccgcgccca
    38641 gacgggcgcg ggattgtggt tgttgggtca ttgcggtagc aagtgaacca taaggaatat
    38701 tcaccaggct gtagcaaagc ccgaggccca tgtaagtcaa atatgcatac actactttgc
    38761 tgctatggct ccagtcggtc ggtacccaga ataccagcac gctgaagatc attaacggcg
    38821 cagtaccgaa gagtaaaaac gggcggaatt ttccccatcg ggtattcaca ctgtccacca
    38881 ctcgtccggc aaagacatcg gcgaaggcat cgaatacccg caccagtaac agcatggtgc
    38941 ccgccgcagc ggcaccgacg ccagcgacgt cggtgtagta actcaacagg aagagcgccc
    39001 ccattgcgaa ggcgaagtta ttggcgacgt caccgaggct gtagccgacg atggcgcgcc
    39061 aggagagttg ttgattcatt gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc
    39121 ggtccagcgt ttttgcagca gaaaagccgc agacttcggt ttgcggtcgc gagtgaagat
    39181 ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt
    39241 ccatacctgc tcaccgacga cagcgctgac gcgatcaaag acgcggtgat acatatccag
    39301 ccatgcacac tgatactctt cactccacat gtcggtgtac attgagtgca ggccggctaa
    39361 cgtatccaca ccgtattcgg tgatgataat cggctgatgc agtttctcct gccaggccag
    39421 aagttctttt tccagaacct tctctgccgt ttccaaatcg ccgctttgga cataccaacc
    39481 gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc
    39541 gcagaacatt acattgacgc aggtgatcgg acgcgtcgga tcgagtttac gcgttgcttc
    39601 cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat
    39661 actccacatc accacgcttg ggtggttttt gtcacgcgca atcagctctt taatcgcctg
    39721 taagtgcgcc tgctgggttt ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt
    39781 gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagctg cagtttcatc
    39841 aatcaccacg atgccatgtt catctgccca gtcgagcatc tcttcagcgt aagggtaatg
    39901 cgaggtacgg taggagttgg ccccaatcca gtccattagt gcgtgatcgt gcaccatcag
    39961 cacgttatcg aatcctttgc cgcgcaaatc cgcatcttca tgacgaccaa agccagtaaa
    40021 gtagaacggt ttgtggttaa tcaggaactg ttcgtccttc actgccactg accggatgcc
    40081 gacgcgcagc gggtagatat cacactctgt ctggcttttg gctgtgacgc acagttcata
    40141 gagataacct tcacccggtt gccagaggtg cggattcacc acttgcaaag tcccgctggt
    40201 gccttgtcca gttgcaacca cctgctgatc cgcatcacgc agttcaacgc tgacatcacc
    40261 atttgctacc acctgccagt caacagacgc gtggttacag tcttgcgcga catgcgtcac
    40321 cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc tgcgatggat
    40381 cccggcatag ttaaagaaat catggaagta agactgcttt ttcttgccgt tttcatcggt
    40441 aatcaccatt cccggcggga tagtctgcca gttcagttca ttgttcacac aaacggtgat
    40501 acgtacactt ttcccggcaa taacatacgg cgtgacatcg gcttcaaacg gcgtatagcc
    40561 accctgatgc tccatcactt cctgattatt tacccacact ttgccgtaat gagtgaccgc
    40621 atcgaaacgc agtacgatac gctggcccgc ccaacctttc ggtataaaga cttcgcgctg
    40681 ataccagacg ttgcccacat aattacgaat atctgcatcg gcgaactgat cgttaaaact
    40741 tcccggcaca gcaattgccc ggatttcttg taacacgctt tcccaccaac gctgatcaat
    40801 tccacagttt tcgcgatcca gactgaatgc ccacagaccg tcgagttttt tgatttcacg
    40861 ggttggggtt tctacaggac gtaacataag ggactcctca ttaagataaa aatactggtc
    40921 aacctttaat ctgattagtt aaccaattct gtgttgcaca cagttatctg tgagaaaccc
    40981 atggaagaga aatggaaata aaaagaactg acaccgggat caaaaaacca aaaactgcaa
    41041 tacccacagc gtgatataga tcgcattaat ctttaaaacg gcgttgacag aagccataaa
    41101 ataagcaacc gaacgttcgt tcgagcataa tgggaacgat aaactctcat gacatatggc
    41161 tacagtgaat attttggggg gaattctccg aacccgggaa ggataatttt tgcacagaga
    41221 aattacgata tgaaacgcgg gcacagttat caccagcccg cgaaatgaga ggatcaggat
    41281 gcggttaaga taccgccaat catcgcccgc aatccctgag caaacgacgc ttcacgcgac
    41341 agatcttccg cttcgatatc ggccagtaca ccgtaggtca gatccagcag taaacgtgaa
    41401 gtcattgccg tgttgatgtc tgggcttatc tcgccttttt gctgggcatc acgcatccgc
    41461 tgggcaacaa attccgtgat cgtcatatgc ttatttttaa gcatggcggc gacctgtggg
    41521 ttacgcatcc cttccgccat aatttcgacc accagcgccc gttgtccaaa ggcctcatgg
    41581 gtgagagaga caatagactc gaccatatag tcaacgaaat gaatcccttc aatcggttcc
    41641 cggaaacggg ccagcgccct ctcctggtcc tgtaagataa tcgcctgaat caaggcttct
    41701 ttggagatga aatggtgata gagcgtcccg ggactaatgg cgcaagattt acagatggct
    41761 ttcatcgagg cactgtgaaa tccattttct gaaaaaatct ctctggcagc attgaggatc
    41821 cgggtccgtg tcggttgtgc ttcagtctgc atgttatcca tcattaaacc attatagtta
    41881 gcgtgaataa ctagtttacc gcgttgtcag gtgagcgcct atcattgctg ttgaatagtg
    41941 cgaaggcaca ctctattcgt atacttttca ctcggaaatt ggttaaccaa tagccgtaat
    42001 ttggctgcca gttatttagc tgagacgttg atgagtttat cccggtaaac aacacgcacg
    42061 gttgtttacc ggtccgttag tgtattaatt gagctcctgt accccaccac cggagacggt
    42121 gagaatttgt ccgcttaccc agctggctgc aggcgagcaa aggaacagcg ccgcgttagc
    42181 aatatcttgc ggttggccca gacgtctgat cggcgtgtgc tgtaacattt tttgttcaat
    42241 ttctggtgta ataacggatt tcagggcatc ggttaatatt gcccccggcg caatgccatt
    42301 cacccgaata tttttttcac ccaggtcaaa cgccatattt ctgaccagat gactggccgc
    42361 agctttagat gatgcataag aagtcatgtt tatattttta ttttctgccg ccatagaagt
    42421 gatggtcaga ataacgccac cgccattttt ttccatttct ggcgcaacaa gttgtgacag
    42481 atggaaaaaa gaaaacacat tcagttcata agcacggcga aaatctgcca ttggcatatc
    42541 aaacggttta ggaccaccgc caccggcgtt gttaaccagg atatcaactt tacccagctt
    42601 actgacggca aagtctgcca gtgcagagag ttcctgttcg gaagtaatat cacaacggca
    42661 ggcaaatgcc tgaccaccca gttgttgaat ttcatcaaca acatggttag ctgcgtcggc
    42721 gttaatatca ctgaccacca cagatgcgcc agctgtcgcg aatgtaatgg cgatttcttt
    42781 accaatacct gcaccggcac ctgtgatgat ggcacatttt ccgtcgagtc tcaggttgtc
    42841 agaattaaac acataaacct cctgtgaact tcagtaaaaa taaaaggcac cgcctgtgtg
    42901 ccacattttt tatcattaac cttgaggaaa ggacactacc aggtatagca gataaagaga
    42961 ggattatttc gccgcaatga gccgggcggg aataataaga ttgcgtgaat gcgtctcttc
    43021 atgggtgatt ttttgcatca tgcgatccgc aagtgtggta ccaagttccc gcgcaggcgt
    43081 gctggcccag gtaacaggta tatcatcaag tgtggttggc gttgcatcgg taaatgccgc
    43141 caacgaaacc tgttgttcaa agtaacgatc gactccgctt tccccgcttt gccgccctgc
    43201 tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg ttatagcaaa ccacagcact
    43261 gatggtcggg ttatgacgta aaagcgccgt gatagcttcc gcggcttgct tctggctgga
    43321 agagcactcc aacacccaat cgctgtgaaa cggcaggcca aattttagta gagttgcaca
    43381 atagcccccc acccgttctg cccgggttaa tgaggaactt tgccctccca gccaggcgat
    43441 acgctgatgc ccattgcgaa tgagatgctc cgtcaacaac tgtgcagcct gcatgttatc
    43501 cgggcgaacc gtatcaacat catcaagata actggcacgg gaagcgaaaa tcaccgggat
    43561 agctttttct tctgccatcc gtcgcaggtc atcgctgctt cctgcagccc cggcaatgac
    43621 cacaccatcg accccctgat tcagtaacag tgaaaaccgc tgtgccagct gctcgccgtc
    43681 tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct tccagagctt ccgtcaatcc
    43741 ggccgttaat tcggcgtaaa acggcgcaga taaatcacgg acgatcaaac caatgacgcc
    43801 gctttgcccg ccgcgcagcg ccgacgcctg acgattgcgc acaaatccca gctcttcaat
    43861 ggcggcgtta acgcgttctc ctgtggcggt agagattcgc cctttgccac ttagcaccag
    43921 cgaaacggtg cttaccgaca cgcccgcagc cagcgcaaca tcatgaatgg ttattttttt
    43981 ggcggtagcc atgcagatga cctactccct gattatgtga cagataaaac gttttacctt
    44041 ttatttatct tatacccgct attatcgttg cgtaatgtga tttatgcctc actaaaattt
    44101 gataaaacgt tttatcttct cgcgcaattt actgaatcca gattgttctc tacgaggagt
    44161 cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt gggagttctt ccagcagtta
    44221 ggcaaaacct tcatgctacc tgtggcatta ttgtcgttct gcggcattat gctcggcatt
    44281 ggtagttctc ttagcagcca tgatgtcatc accctgatcc cggtcctggg caaccccgtg
    44341 ttgcaggcta tctttacctg gatgagtaag attggctcgt ttgcttttag tttcctgcca
    44401 gtcatgttct gtatcgccat cccgctgggc ctggcacgcg aaaacaaagg tgtagcggca
    44461 tttgctggct ttgtcggtta tgcggtaatg aatctcgcgg taaacttctg gttgaccaat
    44521 aaaggcattc tgccaaccac ggacgccgcg gttctgaaag ccaataacat tcagagcatt
    44581 cttgggatcc agtcgatcga tacagggatc ctcggtgcgg tgatcgccgg tattatcgtc
    44641 tggatgctcc atgagcgttt ccataatatc cgcctgccgg atgcgctggc attcttcggc
    44701 ggtacgcgct tcgtgccaat catctcctcg ctggtgatgg gccttgtcgg tctggtgatt
    44761 ccattagtct ggccaatttt cgccatgggt attagcggct taggccatat gatcaacagc
    44821 gcgggtgatt tcggaccgat gctgtttggt accggtgaac gtctgctgtt gccgtttggt
    44881 ctgcatcaca ttctggtggc attaattcgc tttaccgacg caggcggcac gcaggaagtc
    44941 tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg cgcaattgag ttgcccgacc
    45001 actcacggtt tttctgaaag cgccacgcgt ttcctttcgc aaggtaaaat gcctgcgttt
    45061 ctcggcggcc tgccaggtgc agcgttagct atgtatcact gcgcgcgccc ggaaaatcgc
    45121 cataaaatta aaggtctgct tatttctggc ctgatcgcct gtgtcgttgg cggcacgacc
    45181 gaaccgctgg aattcctgtt cctgttcgta gcgccagttc tgtatgtcat ccacgcgctg
    45241 ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg tcaccatcgg taataccgac
    45301 ggcaatatca tcgacttcgt ggtgttcggt attttgcatg gtctgtcaac caagtggtac
    45361 atggtgccag tggtggcggc aatctggttt gtcgtttact acgtcatctt ccgtttcgct
    45421 atcacccgct tcaatctgaa aaccccgggg cgcgatagcg aagttgccag ctcaatcgaa
    45481 aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg ttccggcaat cctcgaagcc
    45541 ttaggcggtg ccgacaatat tgtcagcctc gataactgca ttacccgtct gcgtttgtct
    45601 gtgaaagata tgtcgcttgt taatgtgcag gcactgaagg acaatcgggc aattggcgta
    45661 gtacaactta atcaacataa cctgcaggtt gttatcgggc cacaagttca gtcagtaaaa
    45721 gatgaaatgg ccggtctgat gcatactgtc caggcataag gataagatat gttcgatttt
    45781 tcaaaggtcg tggatcgtca tggcacatgg tgtacgcagt gggattatgt cgctgaccgt
    45841 ttcggcactg ctgacctgtt accgttcacg atttcagaca tggattttgc ctctgccccc
    45901 tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg tgtttggcta cagccgctgg
    45961 aaaaacgatg agtttctcgc ggctattgcc cactggtttt ccacccagca ttacaccgca
    46021 attgatacgc agtcggtggt gtatggccct tctgttatct acatggtctc ggaactgatt
    46081 cgtcagtggt ctgaaaaagg tgaaggcgtg gtgatccaca cacccgccta tgacgcattt
    46141 tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg tcgctttaga gaagcgggct
    46201 gacggctggt tttgcgatat gggcaagctg gaagccgtgt tggcgaaacc agaatgtaaa
    46261 attatgctcc tgtgtagccc acagaatcct accgggaaag tgtggacgtg cgatgaactg
    46321 gagatcatgg ctgacctgtg cgagcgtcat ggtgtgcggg ttatttccga tgaaatccat
    46381 atggatatgg tttggggcga gcagccgcat attccctgga gtaatgtggc gcgcggagac
    46441 tgggcattgc taacgtcggg atcgaaaagt ttcaatattc ccgccctgac cggtgcttac
    46501 gggattatag aaaatagcag cagccgtgat gcctatttat cggcactgaa aggacgtgat
    46561 gggctttctt ccccttcggt actggcgtta actgctcata tcgccgccta tcagcaaggc
    46621 gcaccgtggc tggatgcctt acgcgtctat ctgaaagata acctgacgta tatcgcagat
    46681 aaaatgaacg ccgcgttccc tgaactcaac tggcagatcc cgcaatccac ttatctggcc
    46741 tggcttgatc tacgtccatt gaatattgac gacaacgcgt tgcaaaaagc gcttatcgag
    46801 caagagaagg tcgcgatcat gccggggtat acctacggtg aagaaggtcg tggttttgtc
    46861 cgtctcaatg ccggctgccc gcgttcgaaa ctggaaaaag gtgttgctgg attaattaac
    46921 gccatccgcg ctgttcgtta accccaattg cgcaacgtaa aaaatcgttg cgcaatcgtg
    46981 gatttttacc ctgctttgtt tttataatgg tgcgcacttt tatatccaga aaaagagtgc
    47041 gaccatgatt gataccaccc tgccattaac tgatatccat cgccaccttg atggcaacat
    47101 tcgtccccag accattcttg aacttggccg ccagtataat atctcgcttc ctgcacaatc
    47161 cctggaaaca ctgattcccc acgttcaggt cattgccaac gaacccgatc tggtgagctt
    47221 tctgaccaaa cttgactggg gcgttaaagt tctcgcctct cttgatgctt gccgccgcgt
    47281 ggcatttgaa aacattgaag atgcagcccg taatggcctg cactatgtcg agctgcgttt
    47341 ttcaccgggc tacatggcaa tggcacatca gctgcctgta gcgggtgttg tcgaagcggt
    47401 gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg caggcgaagc ttatcggcat
    47461 tatgagccgg accttcggcg aagccgcctg tcagcaagag ctggaggcct ttttagccca
    47521 ccgtgaccag attaccgcac ttgatttagc cggtgatgaa cttggtttcc cgggaagtct
    47581 gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg catattaccg tccatgcagg
    47641 cgaagctgcc gggccggaaa gcatctggca ggcgattcgt gaactggggg cggaacgtat
    47701 tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg gattttctcg ccgagcagca
    47761 aattggtatt gaatcctgtc tgacctccaa tattcagacc agcaccgtgg cagagctggc
    47821 ggcacatccg ctgaaaacgt tccttgagca tggcattcgt gccagcatta acactgacga
    47881 tcccggcgta cagggagtgg atatcattca cgaatatacc gttgccgcgc cggctgctgg
    47941 gttatcccgc gagcaaatcc gccaggcgca gattaatggt ctggaaatgg ctttcctcag
    48001 cgcagaggaa aaacgcgcac tgcgagaaaa agtcgccgcg aagtaa
//
LOCUS       DAZKJR010000020        47523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKJR010000020 DAZKJR010000000
VERSION     DAZKJR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.020"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(55..786)
                     /gene="rlmB"
                     /locus_tag="ACHOWS_001170"
     CDS             complement(55..786)
                     /gene="rlmB"
                     /locus_tag="ACHOWS_001170"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0046789.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(877..3318)
                     /gene="rnr"
                     /locus_tag="ACHOWS_001171"
     CDS             complement(877..3318)
                     /gene="rnr"
                     /locus_tag="ACHOWS_001171"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0046790.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3357..3782)
                     /gene="nsrR"
                     /locus_tag="ACHOWS_001172"
     CDS             complement(3357..3782)
                     /gene="nsrR"
                     /locus_tag="ACHOWS_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFQ0046791.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3987..5285)
                     /gene="purA"
                     /locus_tag="ACHOWS_001173"
     CDS             complement(3987..5285)
                     /gene="purA"
                     /locus_tag="ACHOWS_001173"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0046792.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5389..5586)
                     /gene="yjeT"
                     /locus_tag="ACHOWS_001174"
     CDS             complement(5389..5586)
                     /gene="yjeT"
                     /locus_tag="ACHOWS_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFQ0046793.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5668..6672)
                     /gene="hflC"
                     /locus_tag="ACHOWS_001175"
     CDS             complement(5668..6672)
                     /gene="hflC"
                     /locus_tag="ACHOWS_001175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFQ0046794.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6675..7934)
                     /gene="hflK"
                     /locus_tag="ACHOWS_001176"
     CDS             complement(6675..7934)
                     /gene="hflK"
                     /locus_tag="ACHOWS_001176"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFQ0046795.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8020..9300)
                     /gene="hflX"
                     /locus_tag="ACHOWS_001177"
     CDS             complement(8020..9300)
                     /gene="hflX"
                     /locus_tag="ACHOWS_001177"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFQ0046796.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGEGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9377..9685)
                     /gene="hfq"
                     /locus_tag="ACHOWS_001178"
     CDS             complement(9377..9685)
                     /gene="hfq"
                     /locus_tag="ACHOWS_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFQ0046797.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9771..10721)
                     /gene="miaA"
                     /locus_tag="ACHOWS_001179"
     CDS             complement(9771..10721)
                     /gene="miaA"
                     /locus_tag="ACHOWS_001179"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0046798.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10714..12561)
                     /gene="mutL"
                     /locus_tag="ACHOWS_001180"
     CDS             complement(10714..12561)
                     /gene="mutL"
                     /locus_tag="ACHOWS_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0046799.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTVEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKMVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPLYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12571..13908)
                     /gene="amiB"
                     /locus_tag="ACHOWS_001181"
     CDS             complement(12571..13908)
                     /gene="amiB"
                     /locus_tag="ACHOWS_001181"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFQ0046800.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPSPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTFLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13927..14388)
                     /gene="tsaE"
                     /locus_tag="ACHOWS_001182"
     CDS             complement(13927..14388)
                     /gene="tsaE"
                     /locus_tag="ACHOWS_001182"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0046801.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14360..15907)
                     /gene="nnr"
                     /locus_tag="ACHOWS_001183"
     CDS             complement(14360..15907)
                     /gene="nnr"
                     /locus_tag="ACHOWS_001183"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFQ0046802.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15906..17045
                     /gene="queG"
                     /locus_tag="ACHOWS_001184"
     CDS             15906..17045
                     /gene="queG"
                     /locus_tag="ACHOWS_001184"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0046803.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17028..17081)
                     /locus_tag="ACHOWS_001185"
     CDS             complement(17028..17081)
                     /locus_tag="ACHOWS_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046804.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17315..17390)
                     /locus_tag="ACHOWS_001186"
     tRNA            complement(17315..17390)
                     /locus_tag="ACHOWS_001186"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17355..17357),aa:Gly,seq:gcc)
     gene            complement(17426..17501)
                     /locus_tag="ACHOWS_001187"
     tRNA            complement(17426..17501)
                     /locus_tag="ACHOWS_001187"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17466..17468),aa:Gly,seq:gcc)
     gene            complement(17659..17734)
                     /locus_tag="ACHOWS_001188"
     tRNA            complement(17659..17734)
                     /locus_tag="ACHOWS_001188"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17699..17701),aa:Gly,seq:gcc)
     gene            complement(17945..18490)
                     /gene="orn"
                     /locus_tag="ACHOWS_001189"
     CDS             complement(17945..18490)
                     /gene="orn"
                     /locus_tag="ACHOWS_001189"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFQ0046805.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18585..19637
                     /gene="rsgA"
                     /locus_tag="ACHOWS_001190"
     CDS             18585..19637
                     /gene="rsgA"
                     /locus_tag="ACHOWS_001190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFQ0046806.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19734..20702
                     /gene="asd"
                     /locus_tag="ACHOWS_001191"
     CDS             19734..20702
                     /gene="asd"
                     /locus_tag="ACHOWS_001191"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFQ0046807.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            20724..24047
                     /gene="mscM"
                     /locus_tag="ACHOWS_001192"
     CDS             20724..24047
                     /gene="mscM"
                     /locus_tag="ACHOWS_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFQ0046808.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24078..24392)
                     /gene="yjeO"
                     /locus_tag="ACHOWS_001193"
     CDS             complement(24078..24392)
                     /gene="yjeO"
                     /locus_tag="ACHOWS_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFQ0046809.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24389..24703)
                     /gene="yjeN"
                     /locus_tag="ACHOWS_001194"
     CDS             complement(24389..24703)
                     /gene="yjeN"
                     /locus_tag="ACHOWS_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFQ0046810.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(24766..26268)
                     /gene="yjeM"
                     /locus_tag="ACHOWS_001195"
     CDS             complement(24766..26268)
                     /gene="yjeM"
                     /locus_tag="ACHOWS_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFQ0046811.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(26487..27464)
                     /gene="epmA"
                     /locus_tag="ACHOWS_001196"
     CDS             complement(26487..27464)
                     /gene="epmA"
                     /locus_tag="ACHOWS_001196"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFQ0046812.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27789..29597
                     /gene="frdA"
                     /locus_tag="ACHOWS_001197"
     CDS             27789..29597
                     /gene="frdA"
                     /locus_tag="ACHOWS_001197"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFQ0046813.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29590..30324
                     /gene="frdB"
                     /locus_tag="ACHOWS_001198"
     CDS             29590..30324
                     /gene="frdB"
                     /locus_tag="ACHOWS_001198"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFQ0046814.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30335..30730
                     /gene="frdC"
                     /locus_tag="ACHOWS_001199"
     CDS             30335..30730
                     /gene="frdC"
                     /locus_tag="ACHOWS_001199"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFQ0046815.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30741..31100
                     /gene="frdD"
                     /locus_tag="ACHOWS_001200"
     CDS             30741..31100
                     /gene="frdD"
                     /locus_tag="ACHOWS_001200"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFQ0046816.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            31163..32296
                     /gene="ampC"
                     /locus_tag="ACHOWS_001201"
     CDS             31163..32296
                     /gene="ampC"
                     /locus_tag="ACHOWS_001201"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFQ0046817.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            32385..32918
                     /gene="blc"
                     /locus_tag="ACHOWS_001202"
     CDS             32385..32918
                     /gene="blc"
                     /locus_tag="ACHOWS_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFQ0046818.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32919..33236)
                     /gene="sugE"
                     /locus_tag="ACHOWS_001203"
     CDS             complement(32919..33236)
                     /gene="sugE"
                     /locus_tag="ACHOWS_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFQ0046819.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33411..33557)
                     /gene="ecnB"
                     /locus_tag="ACHOWS_001204"
     CDS             complement(33411..33557)
                     /gene="ecnB"
                     /locus_tag="ACHOWS_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFQ0046820.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33668..33793)
                     /gene="ecnA"
                     /locus_tag="ACHOWS_001205"
     CDS             complement(33668..33793)
                     /gene="ecnA"
                     /locus_tag="ACHOWS_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFQ0046821.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33845..34411)
                     /gene="efp"
                     /locus_tag="ACHOWS_001206"
     CDS             complement(33845..34411)
                     /gene="efp"
                     /locus_tag="ACHOWS_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0046822.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34453..35481
                     /gene="epmB"
                     /locus_tag="ACHOWS_001207"
     CDS             34453..35481
                     /gene="epmB"
                     /locus_tag="ACHOWS_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFQ0046823.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            35716..36585
                     /gene="yjeJ"
                     /locus_tag="ACHOWS_001208"
     CDS             35716..36585
                     /gene="yjeJ"
                     /locus_tag="ACHOWS_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFQ0046824.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(36635..36988)
                     /gene="yjeI"
                     /locus_tag="ACHOWS_001209"
     CDS             complement(36635..36988)
                     /gene="yjeI"
                     /locus_tag="ACHOWS_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFQ0046825.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(37126..38772)
                     /gene="groL"
                     /locus_tag="ACHOWS_001210"
     CDS             complement(37126..38772)
                     /gene="groL"
                     /locus_tag="ACHOWS_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0046826.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(38816..39109)
                     /gene="groS"
                     /locus_tag="ACHOWS_001211"
     CDS             complement(38816..39109)
                     /gene="groS"
                     /locus_tag="ACHOWS_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0046827.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            39385..40641
                     /gene="yjeH"
                     /locus_tag="ACHOWS_001212"
     CDS             39385..40641
                     /gene="yjeH"
                     /locus_tag="ACHOWS_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFQ0046828.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(40657..41133)
                     /gene="fxsA"
                     /locus_tag="ACHOWS_001213"
     CDS             complement(40657..41133)
                     /gene="fxsA"
                     /locus_tag="ACHOWS_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFQ0046829.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            41470..42906
                     /gene="aspA"
                     /locus_tag="ACHOWS_001214"
     CDS             41470..42906
                     /gene="aspA"
                     /locus_tag="ACHOWS_001214"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFQ0046830.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            43025..44326
                     /gene="dcuA"
                     /locus_tag="ACHOWS_001215"
     CDS             43025..44326
                     /gene="dcuA"
                     /locus_tag="ACHOWS_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFQ0046831.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            44442..44780
                     /gene="cutA"
                     /locus_tag="ACHOWS_001216"
     CDS             44442..44780
                     /gene="cutA"
                     /locus_tag="ACHOWS_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFQ0046832.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            44756..46453
                     /gene="dsbD"
                     /locus_tag="ACHOWS_001217"
     CDS             44756..46453
                     /gene="dsbD"
                     /locus_tag="ACHOWS_001217"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFQ0046833.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIANVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            46490..47065
                     /gene="yjdC"
                     /locus_tag="ACHOWS_001218"
     CDS             46490..47065
                     /gene="yjdC"
                     /locus_tag="ACHOWS_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0046834.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            47172..47247
                     /locus_tag="ACHOWS_001219"
     tRNA            47172..47247
                     /locus_tag="ACHOWS_001219"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47205..47207,aa:Phe,seq:gaa)
BASE COUNT        11329 a        12851 c        11961 g        11382 t
ORIGIN      
        1 tatagacaaa aaaaaaccat ccaaatctgg atggcttttc ataattcaga ggaactagct
       61 gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt tcagggaaga
      121 aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg tcaggcgacg
      181 cataccttca ccttccgcgc ccatcaccag cgccaggcgt ccggtcattt tgctctgata
      241 gagtgtatga tccgcctcgc ctgccgtacc gacgatccag atattctctt cctgcaacat
      301 acgcatggtg cgcgccagat tagtcacccg aatcagcgga acgctttctg ccgcaccaca
      361 ggccactttt ttcgccgtgg cgttgagctg tgcggagcga tctttcggca caatcaccgc
      421 atgaacacca gcggcgtccg cgctacgcag gcacgcgccg aggttgtgcg gatcggttac
      481 gccgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg gcagatcgtt
      541 ttcctgatac tgccgtcctg gcttcacacg agcgataatg ccctgatgca cggcaccgtc
      601 gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct gagactcaag
      661 ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa agacttcctg
      721 aaaacgttca ggggcgcgct ccagcagggc ctgcactgca tggatgccgt aaatcatttc
      781 gctcattaat gtactcgtta tttacgtttt gccgggagcg ccacgcttgc ccggcctacg
      841 gataattcac cctcttctgt ttgaagaggg taatgatcac tctgccactt ttttcttcgc
      901 cgcacgcttc gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc
      961 ttttctcgcg tctttcttcg ctgctttcgg cttcggtttt ttctcgccgc ggaaggcgct
     1021 gtctggctca aagttgacct ttttgccgac ctgacgccgc ttgccgcctt ttttgcctgc
     1081 atcgcctttt ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcgct
     1141 ggagatcagg ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc
     1201 cacgcgatcg cccaggcgat aagtctggcc gctggattcg cccatcaggc gttgccccac
     1261 ctggtcaaag cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa
     1321 caagtcatcc aggcgaacaa agaagccaaa accagtgacg ctggaaatta cgcctttaaa
     1381 gacgttacct acctggtcga gcatgaagtc acacttcagc cagtcagcca catcgcgcgt
     1441 tgcttcgtcg gcacgacgtt ccgccatcga acagtgctga cccagttgca gcatctcttc
     1501 catcgaataa tggtagccgc cggtttcagt ggtattcccc tgatgcccct gctctttcgc
     1561 caacagatac ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt
     1621 aaagtgcgca taggactgca atgccagacc gaagtgacca cggttttccg gatcgtaaat
     1681 cgcttgtttc atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc
     1741 aaccgactcc agcagctctg cgtagtcgcg cggttccggc ttgttaccgc ccggcagttc
     1801 cagccccagc tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc
     1861 gtgaatacgg aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt
     1921 cgccagaatc atgcactctt caattaactt gtgtgcgtcg ttacgctgag tctgttcgat
     1981 acgctcaata cggcgttcgg cgttgaaaat gaacttcgct tcttcgctct caaatgagat
     2041 cccgccacgt tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag
     2101 atgcttaacc agcggggcat actgctcgcg cagatcctga tcgccttgca gtatatgcca
     2161 gactttggtg taggtcagac gcgcgtgaga actcatcacc gcttcgtaga atttgtagcc
     2221 agtcaggcgg cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg
     2281 cgggttgagc gagcacaggc cgttagagag cacttccggc agcatcggga taacctgcga
     2341 agggaagtac accgacgtac cacggttacg cgcttctctg tccagcggtg ttggcggacg
     2401 cacatagtaa ctgacgtcgg caattgcgac ccataaacgc cagccgccgc cgcgtttttt
     2461 ctcacagtaa actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg
     2521 taaatcgcgc agatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc
     2581 ggcaacctgt tgctcaacag cctgcggcca gatgtacgga atttcatggg tacgcagagc
     2641 aatatcaacc gccatgccgg tgcccatatt atcgcccagc acttcgacga ttttacccac
     2701 cgctttggta cggcgagtcg ggcgttgagt cagttcaacc accaccacaa agcccatccg
     2761 cgcgcccatg atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg
     2821 aaccacaaag ccgacgcccg cttcggtgaa gtagcgacca acaatctggc tggtttttgg
     2881 caccagtacg cggacaatac gcgcttcacg acgaccttta cggtcagcgc ccagcggctg
     2941 agccagcacc tgatcgccat gaatgcaggt tttcatctgc tcgctggaga gatacaaatc
     3001 atctttacgc ccttcaactc gcagaaagcc gtagccatca cggtggccaa taacggtacc
     3061 tttcaccagg tcgaggcgtt ccggcagcgc atagcactga cggcgagtga agaccagttg
     3121 accatcgcgc tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc
     3181 aatgtgcagt tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg
     3241 ttcgaggata aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg
     3301 gaaaggatct tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac
     3361 tccaccagca ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag
     3421 ttatccagtt ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag
     3481 gcaggtgtaa tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg
     3541 cgcaccacat caccaatacg tattgcactc gccggtttac ccaagcgaat gccgccattt
     3601 tttccacgaa cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata
     3661 tgattacggg agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct
     3721 ggcaatgacg ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc
     3781 acatcaacct caaaagggaa atcggggaaa aatacattta tattgatgat aaaccagcca
     3841 caagctgtgt cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg
     3901 tgtcggatag cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg
     3961 caaaatatct gccaggcgta ccagaattac gcgtcgaacg ggtcgcgcag aatcatggtt
     4021 tcagtacgat ccggaccagt agagatgata tcgatcggca caccggtcag ctcttcaatg
     4081 cgcttgatgt agttcagtgc cgcctgcggc aggccgctac gatctttcac gccgaaggtg
     4141 gattcagacc agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct
     4201 gccagcggag tggtagtcac ttcacgacca tccggcatac ggtaagccac gcagagttta
     4261 acctctttca ggccatccag aacgtccagt ttagtcaggc agaagccaga cagagagttc
     4321 agctgtaccg cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc
     4381 gtagttgcgc cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt
     4441 tcggtcggga acggacctgc acccacacga gtggagtaag ctttgaggat acccagaacg
     4501 taatcaacat aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg
     4561 gaagaagtta cgtacggata tgtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct
     4621 tcaaacatga cgaaatcgcc acgctgacgc gcctggtcaa gcaggtcaga aacgtcaaca
     4681 accatagaag tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca
     4741 accgcttcag ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc
     4801 agtttttcag cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgcc
     4861 actttatctt cataagcagg cccgatacct cgaccggtgg tgccgatcgc tttcgcgcca
     4921 cgcgctttct cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct
     4981 tcggacagca gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct
     5041 ttcatcagcg cagccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg
     5101 cggagaatac ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta
     5161 tggcctgcgt tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga
     5221 tcgacgatct tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta
     5281 cccatttttc aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt
     5341 acagcacttt ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt
     5401 tttcctcaac atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg
     5461 taaaatatta tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta
     5521 aagcatcggc cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga
     5581 attcattcat tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct
     5641 ttattgacct gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta
     5701 gcggaagaaa tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt
     5761 ctcataagca cgcaggctac ggataaatgc gtagaagtcc ggatctttac tgaatgcatc
     5821 agcaaacagt ttggctgctt ctgcatcgcc ttcaccacgc atgatgcggc cctgacgctc
     5881 agcttcagcc agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc
     5941 ttcctgacct tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt
     6001 gtagatcgct tcagacactt cggtcggcag gttgatctgc ttaatacgca catcgacaac
     6061 ttcaataccc agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc
     6121 agccgttacg cgctctgccg cttcggcaat ggcgttatct gctgccgggg tagtaacttc
     6181 atcttctgta cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc
     6241 acgggaatcg gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc
     6301 agagaactta cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag
     6361 gtagtaacgg ctgaaatcgc tgatgcgcca tttgatgtaa gagtcgacga tcaggtcttt
     6421 cttctctttg gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag
     6481 cattttcacc gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg
     6541 tttgttgtca tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc
     6601 tttgacgaca aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac
     6661 tgacttacgc atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg
     6721 cgttggtcca taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg
     6781 gaggaagagg ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca
     6841 ggggcgttac cacctttcag catctggtct aacggcagaa ccatcaagtt gccaccttta
     6901 tcgttaacca gcactttgcg ggtgttgccc aacacttttt ccatcgtctc gatatacaga
     6961 cgctcgcgag taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc
     7021 acttcaccct gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata
     7081 cgttgcgcct gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga
     7141 atgtattgct gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct
     7201 tccggcggac gagctgcctg gaagttgacg tccagcagcg tgatacccat gtcatacgga
     7261 cgaatcgtct cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc
     7321 agaatgcggt ccatggtgta tttaccgata accccacgca gggcgctgtc ggtagcctga
     7381 cgcaggctgt catccgggct ggtcacgcta tacagatatt tttccggatc ggtgacgcgg
     7441 tactgcacgt tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa
     7501 gcggccagtt cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt
     7561 ttccagttca gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgcca
     7621 cgttcggctt ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc
     7681 gctgcgatgg taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca
     7741 ccgccagatc cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg
     7801 aagatatcat ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca
     7861 gagttgccgc caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc
     7921 tgattccacg ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga
     7981 cgccagagga tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa
     8041 cgccggttct tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact
     8101 tacgctgccg tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact
     8161 tctcagacgc ccttcctgcg gtggcagacg caatgtatgc tgcgccacct cgccggaaag
     8221 ccgctccgtc aaagcctgaa aaagctgtgg tatcccctct ccggtctgtg cggaaagcca
     8281 gacacggatc ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat
     8341 atcgatcttg ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac
     8401 cgtattcacc gcttcgatgt tttcttgtac acgcacatcc gccgcatcaa tgacgtgcag
     8461 cagcaatgtg gcttgccgcg tctcttgtaa cgtggcttta aatgccgcca ccagatcgtg
     8521 cggcaggtgg cgaataaacc ctacggtatc tgccaacacg gtttcaccga catctgcgac
     8581 gtcaatacgc cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg
     8641 cgcttcggtg atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga
     8701 aacagtagga acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac
     8761 tctttccagg cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc
     8821 gagctgggtt tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt
     8881 ccagccacgc accaggcgcg tagccagatg gcgcagctgc gccagctcaa cctgcaactt
     8941 accctcatgg gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat
     9001 aacacgacac tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc
     9061 aaaaagaacg accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc
     9121 ttcacctaca aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc
     9181 ttcgacaccg gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc
     9241 ttgcgtaaaa tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa
     9301 gcgtataacc ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa
     9361 aaaacagccc gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg
     9421 cgctgctacc atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag
     9481 aaaccgggcg agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg
     9541 tgtttttcaa caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac
     9601 cattcaccaa ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat
     9661 cttgtaaaga ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga
     9721 acctttcgat tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg
     9781 atagcaccaa caacctgtaa tacttcgtca cgcgcttgtt ctggtttttc actgtcaagc
     9841 cagtgaaccc cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg
     9901 gcgcaaacac ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac
     9961 atctggcgat aacccacgca acgaatggaa ggcaaatccg tatgcaaatc tcctcgggca
    10021 aaaagcgccc ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca
    10081 attcgttgat ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac
    10141 ggtagagcgt ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa
    10201 acttccagtg cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc
    10261 ggatctacct cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc
    10321 tcaattctgg cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat
    10381 gccttgaaat acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata
    10441 tcggccattt ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga
    10501 tcgcgaatat ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc
    10561 gtcccgatat ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt
    10621 aaaattttac gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa
    10681 aaaatcgcct taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc
    10741 cggatgtaaa tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca
    10801 taaccgttcc acgtccgcca gcagggttat ggcctgcgcc attgaccact gcgcatgttc
    10861 gctcatcaga tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt
    10921 cgccaggtag cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa
    10981 aggcacggcc ctgatggtca catgctgcgc atcggactgg aaatcaatac ccaattccgc
    11041 cagggcagac tgcgcttttt ctaatgcaga tttttcttcg ccagaaactt ttagccgcag
    11101 cggaatcagc aacggctggg cgcaaacggg cgcttcaccc ggcgtcaatt gtgcctgacg
    11161 cagccaacgt tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa
    11221 cgcacagtcg gaatggacga tagtcagtac ccgaccaaag ctttgactgt tcgccgcaag
    11281 tgcaggttcc tgcggttctg gcgcttttgg tttttgcatc ggcgcgggcg tttgcaaaag
    11341 ctggcgatac acttcacctt gctgtttctg gtaacctggc tgcgcattcg gccagggggc
    11401 agccggacga ctgcctgagg cgggcgcagg agtgtaaagc ggagctactg gctcacgaac
    11461 tgccggttca gcaaaatgat tgcgccccgc cgccacgcgg ttttccggga tggcgcgcgg
    11521 tgcaggttgt ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct
    11581 cagcacgccc tgatagataa agtcatgcac cagacgcgac tgatggaaac gcacttcgtg
    11641 tttggcgggg tgtacgttga cgtccacctg atgcgggtcg atctccagat acagcacaaa
    11701 cgccggttgt tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg catgattgat
    11761 caggcgatcg cgcatcatgc gaccattcac gtagcaatac tgaatttccg ccagtgcggg
    11821 cgtggtgtga ttcggatctg ccacccagcc gcgcagcgta agatcgccgt gttgccattc
    11881 aatcgccagc gcttgttcga gaaaagcggt gccgcaaatc gcccctaaac ggcgctcttt
    11941 ttgtccacct tccggcacgg cgcggtactg gcggaccatt ttcccgttat gcgacaggtt
    12001 gatcgtgacg tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa
    12061 ttcggttttc tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag
    12121 cacctccagc gtagtcccca caggatgcgc cgccggtttt accgtcacgt ccatatcgcg
    12181 cccttcggca taggcctgcc aggcttcctg ctgttctacg gtgcgtgaag tgagcgtcag
    12241 gcgggaaacc gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc
    12301 ttcgagatcg tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag
    12361 ctcatctttt ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccgccacg
    12421 ttcgatatca atatcaatgc gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt
    12481 gactaccgac gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg
    12541 cggtaagacc tgtattggca tcaatcgtcc ttagtttggc agcgtacgat ctggcgtcgt
    12601 caccgtactg gcagtttgtg ccgttgcacc ctgcggcgca gattgcatcg gatgcgcaag
    12661 gaaataattg cgcagacctt tataaatggc ttctgccagc tgttgttggt aatcgtcgct
    12721 cgccagcaaa cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgatgg
    12781 gatatccggc gaacgcagaa cgccaaggct ggcgtgttct ggtcgacgtt tatgtatttc
    12841 gccaatgcgt tgcaactggc tgatcatact ggtcgctaca tcatacccta cacgctggga
    12901 atgaccgaac tgtaaatcca gcaccgcctg gcttaaatag ggatcagact gactgttcgc
    12961 cagcacatcg cccgctccgc ccagcagctc cgactgtttc tcgtgctgct ccagccagct
    13021 ggccatttcg ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact
    13081 gcggtttggt gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc
    13141 cacatcgctg cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt
    13201 aaacattgga tcgtcattga gaaaagtacg caatttacgt gcgatggcga tggtgacatt
    13261 tttctcccgc gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtcctgcatc
    13321 aatagcgatg ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac
    13381 cgtattactg ctgataacac ctgtagtgcg gttactttcc gttttaaacg gattgcgcgc
    13441 cggttcgctg acgcgcggtg cgacaaccgc aggcgtttca acgcgtttag caaccacagg
    13501 cggcgaagga ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct
    13561 gccattctgc cgcttcaccg cttcggtttt accgttttcg gttagatcga ccaccagccg
    13621 tagcgtttgt gcatctttag gcgttccgga gcgaatcgcc ttcaccagat tattgccgct
    13681 aaacaacaac ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg
    13741 tttgctttga tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc
    13801 ctgctgattg ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgcgca
    13861 cagcagcagc aacgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt
    13921 caaaggttaa ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac
    13981 gcgcgcctca cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag
    14041 aacacctgta ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc
    14101 gcggatcccc ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata
    14161 aaccattaaa ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt
    14221 accctgatgg cccaaagcct gcaaaaagcc ccggctaaag gtggttttac ctgcgcctaa
    14281 atcgccatac agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag
    14341 gtctaatgtt gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag
    14401 tcacttccgg gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc
    14461 gcgttccaaa acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac
    14521 aggctgcatc atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca
    14581 catcgcccat gccgccgctc gccatgcctg cattcccggc atcaataatg cctaaagcgt
    14641 caggatgggc ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt
    14701 gtaccagacg tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta
    14761 acaaccgtgc ggcctcgcca ggatgtggcg tgatcacgcg attgtgacgc ttatcgggat
    14821 tgattgccag caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa
    14881 ctttttgcag ggcttttttc ccccactctt gctggcccag accgggacca atcaccacca
    14941 catcggccca ttccaggctt tcggtaagag agtccatagt cagttcatgc accatcaatt
    15001 ctggtcgtgc agtcagcagc ggcgcaatgt tctcactgcg ggtcagtact cggactaaac
    15061 cagcaccagc acgtagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat
    15121 cgccaccgat aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggta
    15181 tcagccactg agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca
    15241 gccagctatc cagccccagt gagtcaaaat gcagttgtcc ggtaacatcc cgcgcttttc
    15301 cagtgagcaa gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgattaccg
    15361 cgcctggcgt agcgccggtt tcagccagaa ggccggaagg gatatcaacc gccacaatcg
    15421 gcgcagggtg agtattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct
    15481 gcaagccggt gccgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat
    15541 tcgaagcatg gatctcgcct cccgcgttta accatgcttc gcgtgccagc gcggcctctt
    15601 ccggcaacgg tttgtcactt tcctgggcca gcagcgtgac atcaataccg atcgctttgg
    15661 ccagccgcgc gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc
    15721 agtggcgggc gtcaggataa gccgaacgac acacctggaa tgcggcttcg ccagcgcgaa
    15781 gcatcagctc atagagtgtg agccccagcg catctgccgc ctcgcgttct ccgcggcgga
    15841 tatcgtcggc gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt
    15901 ccgtcatgtc agagcccctc gatctcaatc aattagcgca aaatattaaa cagtgggggc
    15961 tggaactggg ctttcagcag gtaggtatta ccgataccga tctcagcgcg tccgagccca
    16021 aactgcaagc atggctggac aaacaatacc acggcgaaat ggactggatg gcgcgtcacg
    16081 gtatgctgcg tgctcgtcct cacgaattat tgcccggtac gctgcgcgtg atcagcgttc
    16141 ggatgaatta ccttcctgct aacgccgcat ttgccagcac gctgaaaaat cccaaactcg
    16201 gctatgttag ccgttatgcg ctgggccgtg actatcacaa gcttctgcgc aaccgactca
    16261 aaaagctggg cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg
    16321 attctgcgcc tattctcgag cgcccgttag ctgaaaaagc aggtcttggc tggacaggta
    16381 agcactcact tatcctcaat cgcgaggccg gttcgttctt ctttttaggc gaattgctgg
    16441 tcgatattcc gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcgtggcct
    16501 gtatgacgat ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct
    16561 gtatctctta tctcaccatc gaactggaag gggcgatccc ggaagagttg cgaccgttaa
    16621 tgggaaaccg tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt
    16681 cgcaactcac tacagaagac gatttcagcc cgcgtaagcc gctacacgca ccggaactca
    16741 ttgagttatt cgcctggagt gaagagaagt ttttaaaagt cacggaaggt tcggcgattc
    16801 gccgtatagg tcacctgcgt tggctgcgta atatcgccgt agcattaggc aatgccccct
    16861 gggatgaaac gattttgaca gcgctggaaa gtcgtaaagg tgagcaccca cttcttgatg
    16921 agcacatagc gtgggcgatt gcgcagcaaa tcgagaggcg aaatgcgtgc gtggtcgaag
    16981 tgcaattacc caaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg
    17041 cctgattcat ccacagcttg tgaataaaaa taaaaacgta ttgcaattca agacgaataa
    17101 aatcgtcaag tgatcgactc gacaaaacga aatcttaatt tttaacttat ctatcaatag
    17161 gttaaaaaaa tactattcac cgtgcgaagt ttctttgaaa ttaagattac ggcctgaggc
    17221 tgtggataac tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc
    17281 gcgtcgctgt ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc
    17341 gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc
    17401 tgtgctttac agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg
    17461 accttggcaa ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa
    17521 ccgctactgc cttggctgcc ttcactcacc ttagtcactt acttttgtaa gttcccaagg
    17581 attcgttcag ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt
    17641 gcagcacttt tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg
    17701 caaggtcgtg ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat
    17761 tttcgcattt ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac
    17821 ccccctaaaa aatttttttt gagtttttat ctcaagtgct gaaattatca gcaagacgtt
    17881 caatttagcg tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa
    17941 aattttacag cttgataaaa tgctcgcggt agtaagccag ttctgccacc gactcacgga
    18001 tatcatccat cgcctgatgc gtcccctgct tggtaaaacc atcaagaatt tccggcttcc
    18061 agcggcgcgc cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt
    18121 ccagctccgg catatattta aacaggaagc gacgatcctg gccgatgctg ttaccgcaaa
    18181 tcggcgattt tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt
    18241 cacgatcgcc catcgtgctc gctttcacgc gctccaccag cccgctggcg gtatgggtgc
    18301 gcacgttcca gtcatccatc agcgccagct gttcatcaga ctggtgtact gcaatggtcg
    18361 gcccttctgc cagaatattc aggttggcat cggtcaccag cgtggcaatc tcaataatgc
    18421 gatcgcgctc gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat
    18481 tggcactcat gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta
    18541 tcatagatgt tttgcccatc aggggcgacc aggagtctgt acgattgagt aaaaataaac
    18601 tctccaaagg ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta
    18661 aggagaagcc cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca
    18721 gccgctttgg tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata
    18781 ttcgccgtac catccgttcg ttggtgaccg gcgaccgcgt agtctggcgt ccgggtaaac
    18841 cagcggcgga aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgcacctcag
    18901 tgttgacgcg cccggatttt tacgacggtg tgaaacctat tgctgccaac atcgaccaga
    18961 ttgtcattgt ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctgg
    19021 tggcctgcga aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg
    19081 acgacgaagg catggcattc gtcaacgagc agatggatat ctaccgcaat atcggttatc
    19141 gcgtattgat ggtttccagt catactcagg atgggctgaa accgctggaa gaggcgttga
    19201 ccgggcgcat cagcattttt gccgggcagt ctggcgttgg caaatccagc ctgctgaacg
    19261 cattgctggg tctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcgggtctcg
    19321 gtcagcacac cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg
    19381 actccccagg agtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg
    19441 gctttgtcga attccatgac tacttaggtc tgtgtaaata tcgcgattgc aaacacgata
    19501 ccgatccggg ctgcgctatc cgggaagcgg ttgaggaagg aaaaatcgcg gaaacccgtt
    19561 tcgaaaacta tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt
    19621 ctgatacgga tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg
    19681 atccggcatg taggccggat caggaacgac aaaacaatgg cctggaggct accttgttaa
    19741 attcatttaa actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg
    19801 gttggggcgc aagcaagcgg gcaggatggc tgacaaaact ggttatcgat ctgttcgtta
    19861 aatactacaa ggtcgatatg aaagaggcgc aaaagccgga caccgccagc taccgcacct
    19921 ttaacgaatt ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga
    19981 atgtactggt tatgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata
    20041 aaatcctgca agccaaaggc cacaactaca gcctcgaagc cctgctggct ggcaactatc
    20101 tgatggcgga cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccacgtgact
    20161 accaccgcgt acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg
    20221 atctcttttc cgttaaccat ctcaccgctc agaacgtgcc gaatctgttt gcccgtaacg
    20281 aacgcgtgat ctgcctgttc gataccgaat ttggcccaat ggcgcagatt ctggtcgggg
    20341 cgacgattgt aggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag
    20401 gtatcatcaa gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga
    20461 aaggccagga aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg
    20521 gtaaagtgaa tctggttgag caactggaaa gcctgtctgt cacgaaaatt ggccagccgc
    20581 tggcagtatc taccgaaacc tttgttacac cagacgctga accggccccg cttcctgctg
    20641 aagagatcga agcagaacac gacgccaacc cattggttga cgacaacaaa gaccaggtct
    20701 aatccatcaa aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc
    20761 tcagttgggg ggcgtacgcc gcgacggccc ccgatagcaa acaaatctca caagaactgg
    20821 agcaggcaaa agcggcgaaa cccgcgcagc cggaagtcgt agaggcgctc cagtccgcct
    20881 taaatgcgct tgaggaacga aaaggttccc ttgagcgcat caagcaatat caagaagtca
    20941 ttgataatta cccgaaactt tccgctactc tgcgcgcaca attaaacaac atgcgtgacg
    21001 aaccgcgcag cgtgtcaccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg
    21061 tcagcagcca gttgctggat aaaagccgtc aggcccagca agagcaggaa cgcgcccgcg
    21121 agattgccga ttcgctgaat caactgccgc aacagcaaac cgatgcccgc cgtcagttaa
    21181 atgaaatcga gcgccgcctg gggacgctta ccggcaattc cccgctcaat caggcacaaa
    21241 atttcgcgtt gcagtctgac tccgcacgcc tgaaagcgct cgtcgatgaa ctggaactgg
    21301 cgcagctgtc tgccaataac cgtcaggaat tagcgcgctt acgctcagag ctggcggaaa
    21361 aagagagcca gcaactggat gcgtatttgc aggccttacg taatcagttg aacagccaac
    21421 gtcagcttga ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg
    21481 atttgccgaa agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga
    21541 atcaacaggc gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga
    21601 cgttacaggt ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggctcgt
    21661 ccaacctgct cggcgaagcg ttgcgggcgc aggtggcacg gctaccggaa atgccaaaac
    21721 cacaacagct tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc
    21781 tgctaaataa acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg
    21841 ccgagcaaaa ccgtattctg gaagcacagc tacgcactca gcgtgagttg ctgaactcat
    21901 tgttgcaggg tggagacacg ctactactgg aactgaccaa gctgaaagtc tccaacgggc
    21961 aactggagga tgcgctgaaa gaggtgaatg aagcgacgca ccgctatctg ttctggacct
    22021 ctgacgtgcg cccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc
    22081 tcatttcgct ggacaccttc agtcagttgg gcaaagccag tgtaatgatg ctgaccagta
    22141 aagagacgat tttgccgctg cttggcgcgt tgattctggt cggttgcagt atttactcgc
    22201 gccgctattt cacccgtttt cttgaacgtt cggcggcgaa agtcggcaaa gtgactcagg
    22261 atcacttctg gctgacgtta cgcactcttt tctggtcgat tctcgtcgcg tcaccgctgc
    22321 ctgtgctgtg gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg
    22381 tcgcgattgg tgatggcgta acagccaccg tgccgctgct gtgggtagtg atgatttgcg
    22441 ccacctttgc ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacggg
    22501 tttcccgtgg gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg
    22561 cgctgatgat gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggacggcttt
    22621 gctttatcct catttgcggt gcgctggcgg tggtcactct cagtctgaaa aaggccggga
    22681 tcccgctgta tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga
    22741 acatgatgat tggcgcaccg ctggttgcca ttctggcgtc ggcggtgggt tatctggcaa
    22801 cggcgcaggc gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg
    22861 tggtttatca cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc
    22921 gggcgaagca tcgccgggca gagatgttag cgcagcgtgc gcgtggcgaa gaggaagcgc
    22981 atcatcacag tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg
    23041 ccatcagtgc gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt
    23101 cggtcattgt gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc
    23161 tgtgggatgt cacttccacg gtacagggcg tagaaagcct ggagccgatt accctcggtg
    23221 cggtgctgat tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg
    23281 cgctgctgga actggcgatt ctgcagcacc tggatttaac gccgggtacg ggctacgcca
    23341 tcaccaccat caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga
    23401 ttggtattga gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt
    23461 ttggtttgca ggaaattttc gccaacttta tctctggcct gattatcctg ttcgaaaaac
    23521 cgattcgcat tggcgatacg gtaacgatcc gcgatcttac cggtagtgtg acgaaaatta
    23581 acacccgcgc caccaccatc agcgactggg accgcaaaga aattatcgtg ccgaacaagg
    23641 cgtttattac cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtggtgt
    23701 tgacgattcc ggcccctgcc gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg
    23761 cagcgcgtcg ctgctcgctg gtaatcgata acccagcacc ggaagtcttc ctggtggatc
    23821 tgcaacaggg gattcaaatt ttcgagctgc gtatttacgc cgctgagatg ggtcaccgta
    23881 tgccactacg ccatgagatt caccagctaa ttctggcagg attccatgcc cacggtattg
    23941 atatgccatt cccgcccttc cagatgcgtt tggaaagcct gaatggtaag caaacgggga
    24001 gaacattaac ttctgcgggc aaaggtcgtc aggcgggaag tttgtaaggt ctttgtggcc
    24061 ggatgaaccc atccggctta taagcaaaac tgatagcgca gaaaaaactg ccacagccaa
    24121 tagccgaaaa tagccagcgc ggtggcgtac ataaaccagc cgcgtttttt tcgccacaaa
    24181 gcgaaccaga gaaaagggaa aaagagcggt aacgtcatta tcacaccgac atcacgggta
    24241 tcgtcatctt cgagatacat cagcacatcg cagacgttat taatcccgcg tccatcaatc
    24301 atccactctt tgtccagcgc ggaagtgatg gttatccaga ggaacgccac aataaaccag
    24361 atgcagcaca agataaacat gcgcgcactc atgagataat cggtcgctta atttcgctat
    24421 agcgcatacg aaacaaatcg ccgtatccca ctaattctcc gcgaaaaacc agacatctaa
    24481 taagataagc gcaatagata tccggcaggt ttccccaccg cggaccatcg cgctctttga
    24541 gggtattaat agtgttaccc accaccatcg ctactttgcg ggtgtggcgg gtatcgacaa
    24601 aagagaggac cgtttgctct atttccgcca catccccggc agaaaattgc agctccctga
    24661 tttcatcttc ggttatcact ggatcccgac tagcgtcgtc cataacgtct tcctttccag
    24721 acaactcatc gccggagaga cgatgagttg caggatttca tatgatcagt cgagagctaa
    24781 ctcaggtttg ttcgccatgc gactgcaata gttctggtaa atcgccatcg ctaacagcga
    24841 gaagaagaca gggccgccaa tcatccacaa tgtgctgtcc cagtctccag cttcaaccac
    24901 aggttgaatg atggtgaaga cgttcgcaaa tgtcaccacc agtacgacaa ccactgttgc
    24961 aatcattgcc gacatatgcg ttttgaaaat cacaaacggt ctgtcgagat cctgacgtgc
    25021 tttaaagaat gggaaagcca gcgcgaggaa caggtaagga agcgtcatag acacgttcgc
    25081 catcagcgtc agcttgttaa agaacgccga tgcggtaccg ccaccaaacg aaaccagcag
    25141 gatgaagaca gtaaccaacc cgcactgcat ccacatggcg atagacggca tccccatcgc
    25201 attcaggcgc gtcatcggtt ccggccacaa cgctttcggc gtcccctgga tgatggcttt
    25261 cagcggtgaa tagcaaagcg taaagaacgc accggtataa gcgaggaaca tcgaaagtcc
    25321 ggtaatacgc gcaaaccata cgcccagcga caatgacgct tcaggtgaca aatgcagtgc
    25381 gttacccagc gtcaccccga ggctcttcat cagcacatag gtaatattgc cgaggttaac
    25441 ggaaccatta cttaatacct gctgccagtt tgtgctgacg ccccataaaa atattgccag
    25501 cgaataaccg attgaaataa caatagcggc aaaaacaata cctttggcaa agttcttttc
    25561 tggattttcc gttttatcga ccagaccacc aaccgcttca attccaccat aggcaaaaat
    25621 agcaaatacc acaaacgata gcattgccag accggactga taacctgggt tcggtgatgc
    25681 aaggaaatta atatcctgcg cgaaatgccc accatttaat aacaaaatag taatgcttac
    25741 taacagcaat actaaattca gacacattac tgcaataccg cccaccgcag taatgcgggc
    25801 aattttatta atcccttttg aagcaacgac ggtgaccaga atcatccatg ccactgccag
    25861 cagaccaacc acctgcgtag gctccagtcc ggcaatacgc cagtgctggg tcatgtcgct
    25921 accatagagg aatgttgaga acggtaccca aactttcgcg gaggtactca ccatccagat
    25981 gatataagag gaaaaccaca taaacgtacc aatgaaggca aaacgtggtc cgacactatt
    26041 attcatccag gaatagatac cgccttcttc tttgcgataa gcagctccca tttcagccat
    26101 cattaaggcg aatggaataa agaataacaa tgcagaaaat atataaaagg gaatcgcact
    26161 ataacccatt aagtaataag ccgatgggct attggcaaat ccaaatacgg aagtaaagat
    26221 catcaatatg agtcctatca gactcatctt ttttatcgtg tgaggcatga aaacatcctt
    26281 catttcagcg cgtgtataaa acgcgcccac tgaactgcac tgcccaacat tgagcgggca
    26341 gcgtaaaaac caatacagaa ttgatacctg gatgatatca aatatccagt cttaaattgt
    26401 ggcctttagc ggaaaaatga aaacatatgc tacagaatgg cgcttatcac gccattcttc
    26461 gcggttaatt cagtaatttt tcagaattat gcccggtcaa cgctaaaggc gatgacttca
    26521 gccagtgtct ccgcgcccag cgccaacatc accagacgat caacgcctaa tgccacgccg
    26581 gaacagtcag gcataccgac tttcaaggct tcaatcagat tctggtcaat ggggtgctgc
    26641 ggcaaaccgc gcgccgcgcg cttacggtta tcttgttcaa agcgttgttg ctgctcacgg
    26701 gcatccgtca attcatggaa accattcgcc agctcaatac ctttataata aacctcaaag
    26761 cgttcagcga cccgatgatc ttcggtactg atttgcgcca gtgatgcctg gctggctgga
    26821 aagtggtaca caaaggtcgg tttttctttg ccaatatttg gctctacgcc aaaggtaaac
    26881 agcaattgta gaagcgtgtc gcggtcttct tcagtatcgg caacgttgct caaatccagt
    26941 ttcgctgcca cttcccgcag ttgcgttttg tcggcagaga gtgggtcaat ttccagataa
    27001 cgcaagaaag cttgttgata agaaaggctt tctgctgccg gacagtccag cacctgttgc
    27061 aagagatcgt ccacctcgtt catcaaccgg tacatatcat agtgcggtcg ataccactca
    27121 agcatagtga actcagggtt gtgataacgc cccatctctt cattacggaa gctgcggcac
    27181 agctggaata ccggcccaca accagcaacc agcaggcgtt tcatatggta ttccgggctg
    27241 gtcattaacc agagattcat cccctgcgaa tgcccggggc ctacgaaacg tgtctcaaac
    27301 gggaccaaat gaatatcggt taccgtcgcc tggctcatac aaggcgtctc cacctccagc
    27361 actccacgat cggcaaagaa acgacggatc tccgccataa tcgccgcgcg ttttaataag
    27421 ttaggaatgg atgcgctcgg ctgccaggat gccgtttcgc tcatagttaa atctccagtt
    27481 tttgacaagg gcacgaagtc tactcgcaac gcgacggcga gacaaatttt acgcaggaat
    27541 caaacaaggg tgggcagtga ctaaaaaaag cacgatctga tggtttagta attaaattaa
    27601 tcatcttcag tgataattta gccctcttgc gcactaaaaa aatcgatctc gtcaaatttc
    27661 agacttatcc atcagactat actgttgtac ctataaagga gcagtggaat agcgttcgca
    27721 gaccgtaact ttcaggcact taccctgaag tacggggctg tgggataaaa acaatctgga
    27781 ggaatgtcgt gcaaaccttt caagccgatc ttgccattgt aggcgccggt ggcgcgggat
    27841 tacgtgctgc aattgctgcc gcgcaggcaa atccaaatgc aaaaatcgca ctaatctcaa
    27901 aagtataccc gatgcgtagc cataccgttg ctgcagaagg gggctccgcc gctgtcgcgc
    27961 aggatcatga cagcttcgaa tatcactttc acgatacagt agcgggtggc gactggttgt
    28021 gtgagcagga tgtcgtggat tatttcgtcc accactgccc caccgaaatg acccaactgg
    28081 aactgtgggg atgcccatgg agccgtcgcc cggatggtag cgtcaacgta cgtcgcttcg
    28141 gcggcatgaa aatcgagcgc acctggttcg ccgccgataa gaccggcttc catatgctgc
    28201 acacgctgtt ccagacctct ctgcaattcc cgcagatcca gcgttttgac gaacatttcg
    28261 tactggatat tctggttgat gatggtcatg ttcgcggcct ggtagcaatg aacatgatgg
    28321 aaggcacgct ggtgcagatc cgtgctaacg cggtcgttat ggctaccggc ggtgcgggtc
    28381 gcgtttatcg ttacaatacc aatggcggca tcgttaccgg tgacggtatg ggtatggcgc
    28441 taagccacgg cgttccgctg cgtgacatgg aattcgttca gtatcaccca accggtctgc
    28501 caggttccgg tatcctgatg accgaaggct gccgcggtga aggcggtatt ctggtcaaca
    28561 aaaatggcta ccgttatctg caagattacg gcatgggccc ggaaactccg ctgggcgagc
    28621 cgaaaaacaa atatatggaa ctgggtccac gcgacaaagt ttctcaggcc ttctggcacg
    28681 aatggcgtaa aggcaacact atctccacgc cgcgtggcga tgtggtttat ctcgacttgc
    28741 gtcacctcgg cgagaaaaaa ctgcatgaac gtctgccgtt catctgcgaa ctggcgaaag
    28801 cgtacgttgg cgtcgatccg gttaaagaac cgattccggt acgtccgacc gcacactaca
    28861 ccatgggcgg tatcgaaacc gatcagaact gtgaaacccg cattaaaggt ctgttcgccg
    28921 tgggtgaatg ttcctctgtt ggtctgcacg gtgcaaaccg tctgggttct aactccctgg
    28981 cggaactggt ggtcttcggc cgtctggccg gtgaacaagc gacagagcgt gcagcaactg
    29041 ccggtaatgg caacgaagcg gtaattgaag cgcaggcagc tggcgttgaa caacgtttga
    29101 aagatctggt taaccaggat ggcggcgaaa actgggcgaa gatccgcgac gaaatgggcc
    29161 tggctatgga agaaggctgc ggtatctacc gtacgccgga actgatgcag aaaaccatcg
    29221 acaagctggc agagctgcag gaacgcttca aacgcgtgcg catcaccgac acttccagcg
    29281 tgttcaacac cgacctgctc tacaccattg aactgggcca cggtctgaac gttgctgaat
    29341 gtatggcgca ctccgcaatg gcacgtaaag agtcccgcgg cgcacaccag cgtctggacg
    29401 aaggttgcac cgagcgtgac gacgtcaact tcctcaaaca caccctcgcc ttccgcgatg
    29461 ctgatggcac gactcgcctg gagtacagcg acgtgaagat tactacgctg ccgccagcta
    29521 aacgcgttta cggtggcgaa gcggatgcag ccgataaggc ggaagcagcc aataagaagg
    29581 agaaggcgaa tggctgagat gaaaaacctg aaaattgagg tggtgcgcta taacccggaa
    29641 gtcgataccg caccgcatag cgcattctat gaagtgcctt atgacgcaac tacctcatta
    29701 ctggatgcgc tgggctacat caaagacaac ctggcaccgg acctgagcta ccgctggtcc
    29761 tgccgtatgg cgatttgtgg ttcctgcggc atgatggtta acaacgtgcc aaaactggca
    29821 tgtaaaacct tcctgcgtga ttacaccgac ggtatgaagg ttgaagcgtt agctaacttc
    29881 ccgattgaac gcgatctggt ggtcgatatg acccacttca tcgaaagtct ggaagcgatc
    29941 aaaccgtaca tcatcggcaa ctcccgcacc gcggatcagg gtactaacat ccagaccccg
    30001 gcgcagatgg ccaagtatca ccagttctcc ggttgcatca actgtggtct gtgctacgcc
    30061 gcgtgcccgc agtttggcct gaacccagag ttcatcggtc cggctgccat tacgctggcg
    30121 catcgttata acgaagatag ccgcgaccac ggtaagaagg agcgtatggc gcagttgaac
    30181 agccagaacg gcgtatggag ctgtactttc gtgggctact gctccgaagt ctgcccgaaa
    30241 cacgtcgatc cggctgcggc cattcagcag ggcaaagtag aaagttcgaa agactttctt
    30301 atcgcgaccc tgaaaccacg ctaaggagtg caacatgacg actaaacgta aaccgtatgt
    30361 acggccaatg acgtccacct ggtggaaaaa attgccgttt tatcgcttct acatgctgcg
    30421 cgaaggcacg gcggttccgg ctgtgtggtt cagcattgaa ctgattttcg ggctgtttgc
    30481 cctgaaaaat ggcccggaag cctgggcggg attcgtcgac tttttacaaa acccggttat
    30541 cgtgatcatt aacctgatca ctctggcggc agccctgctg cacaccaaaa cctggtttga
    30601 gctggcacca aaagcggcca atatcattgt aaaagacgaa aaaatgggac cagagccaat
    30661 tatcaaaagc ctctgggcgg taactgtggt tgccaccatc gtaatcctgt ttgttgccct
    30721 gtactggtaa ggagcctgag atgattaatc caaatccaaa gcgttctgac gaaccggtat
    30781 tctggggcct cttcggggcc ggtggtatgt ggagcgcaat tattgcgccg gtgatgatcc
    30841 tgctggttgg tattctgctg ccactggggc tgtttccggg tgacgcactg agctacgagc
    30901 gcgttctggc gttcgcgcag agcttcattg gtcgcgtatt cctgttcctg atgatcgttc
    30961 tgccgctgtg gtgtggttta caccgtatgc accacgcgat gcacgatctg aaaatccacg
    31021 tacctgcggg caaatgggtt ttctacggtc tggctgctat cctgacagtt gtcacgctga
    31081 ttggtgtcgt tacaatctaa cgcatcgcca atgtaaatcc ggcccgccta tggcgggccg
    31141 ttttgtatgg aaaccagacc ctatgttcaa aatgacgctc tgcgccttat taattaccgc
    31201 ctcttgctcc acatttgctg cccctcaaca aatcaacgat attgtgcatc gcacaattac
    31261 cccgcttata gagcaacaaa agatccccgg tatagcggtg gcggtaattt atcagggtaa
    31321 accttattac tttacctggg gctatgcgga catcgccaaa aagcagcccg tcacacagca
    31381 aacgttgttt gagttaggtt cggtcagcaa aacatttacg ggcgtgcttg gtggcgacgc
    31441 tattgctcga ggggaaatca agttaagcga tcccgcgaca aaatactggc ctgaacttac
    31501 cgctaaacaa tggaatggga tcacactatt acatctcgcg acctacaccg ctggcggcct
    31561 gccattgcag gtgccggatg acgttaaatc ctcaagcgac ttgctgcgct tctatcaaaa
    31621 ctggcaacct gcatgggctc caggaacaca acgtctgtat gccaacgcca gtattgggtt
    31681 gttcggtgca ctggcggtga aaccatccgg tttgagcttt gaacaggcga tgcaaactcg
    31741 tgttttccag ccactcaaac tcactcatac gtggattaat gtgccgtccg cagaagaaaa
    31801 gaattacgcc tggggatatc gcgaaggtaa ggcagtgcat gtttcgccag gggcgttaga
    31861 tgctgaggct tatggtgtga agtcgaccat tgaagatatg gcccgctggg tgcaaagcaa
    31921 tttaaaaccc cttgatatca ctgagaaaac gcttcaacaa gggatacaac tggcacaatc
    31981 tcgctactgg caaaccggcg atatgtatca gggcctgggc tgggaaatgc tggactggcc
    32041 ggtcaatcct gacatcatca ttaacggcag tgacaataaa attgcactgg cagcacgccc
    32101 cgtaaaagcg attacgcccc caactcctgc agtacgcgca tcatgggtac ataaaacggg
    32161 ggcgaccggc ggatttggta gctatgtcgc gtttattcca gaaaaagagc tgggtatcgt
    32221 gatgctggct aacaaaaact atcccaatcc agcgagagtc gccgccgcct gtcagattct
    32281 caacgctcta cagtaaaatt tcatcgggtc cgaatttttg gaccttttct ccgcttttcc
    32341 ttgctgtcat ctacacttag aaaaaaacca gtaaggaaac atttatgcgc ctgctccctc
    32401 tcgttgccgc agcgacagct gcatttctgg tcgttgcctg cagttctcct acgccgcccc
    32461 gtggcgtgac cgtagtgaat aatttcgatg ctaaacgcta tctcggtacc tggtatgaga
    32521 ttgcccgttt tgatcaccgt tttgaacgtg gactggaaaa agtcaccgca acatacagcc
    32581 tgcgtgatga cggcggcctg aatgtcatta ataaaggcta taaccctgac agagaaatgt
    32641 ggcagcagag tgaagggaaa gcgtacttta ccggcgcacc aactcgcgct gcgctgaaag
    32701 tgtcattctt tggtcctttc tatggcggtt ataacgttat tgcactcgat cgggaatacc
    32761 gccatgcgct ggtttgcggt ccggaccgcg actacctgtg gatactctcc cgcacgccaa
    32821 ccatttctga cgaagtgaaa caggagatgc tggcagtcgc gacccgggaa gggtttgatg
    32881 tcagtaagtt tatttgggta cagcagcccg gtagttagtt agtgagtgct gagtttcaga
    32941 ccaataatcc ccaatacgat taacgccaga ctcgccaggc gcatcgggtt agcggactcg
    33001 ccgagcagca caatgccggt gatggccgcg ccgactgcgc caatacccgt ccacacggca
    33061 taagccgtcc ctactggtaa cgatttcatc gcccaggcaa gtagcgccat actgacaatc
    33121 atcgccgtca cagtaataac acttggcgtc aaacgactaa agccgtgggt atatttcagg
    33181 ccaacggccc ataccacttc cagcaggcca gcaataacta agataatcca ggacatatca
    33241 ggctccggag aattggggcc gtccccggtg aaaagaggcg tttgcaggtc gtcctgcaaa
    33301 gctgatggtt gaacggatat tttgccaggt ggcaggggaa tttcaactat tacctttgtg
    33361 actatcgcaa aaaaacaaac ggcacgacac agctgtcgta ccgttattgc ttactgctgc
    33421 gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
    33481 cgcgtggtgt tgcaagcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
    33541 attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc
    33601 aaattaataa tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaggaga
    33661 agaaaaatta gctggcagcg cgggagatgg agttgccgag atgtttaatg tcttcaccaa
    33721 aaccgcgagc ggtgttacag cccgtgagca gcgcactggc aagcaaaaca agaacgataa
    33781 ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac
    33841 cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc
    33901 cgatctgtac gaacagcgga actttaacca cagcgccagt agacagggta gctggtttgc
    33961 cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagctcaa
    34021 cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg
    34081 cctgatccag caaccatttg gcgttgtcgc caattgcttt tgcatcagca gacagctgct
    34141 cgaaagtttc gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt
    34201 tcatatcgac aacatcagca ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc
    34261 gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt
    34321 ttacgaattc actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag
    34381 cacgaaaatc gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag
    34441 ctaagccaca aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta
    34501 acgcaacttg ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac
    34561 gcggacgaaa aactgttggc cgggcgcagc gccaaaaagc tgtttgccct gcgtgtgccc
    34621 cgctcattta tcgatcgcat ggagaaaggc aatccgaacg atccgctttt gcgtcaggta
    34681 cttacctcgc aagatgagtt tgttgtcgcg cccggattct ccaccgaccc gctggaagaa
    34741 cagcacagcg tagtgccagg tttgttgcat aaataccaca accgggcgct tttgctggtc
    34801 aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa
    34861 aatcagggca acaagcgtaa ctggcagact gcacttgagt atgttgctgc gcatccggaa
    34921 ctggacgaga tgattttctc cggcggcgat ccgctgatgg cgaaagatca cgagctggac
    34981 tggttgctca cacaactgga agccatcccg catatcaaac gtctgcggat tcacagccgt
    35041 ctgccgattg tgatcccggc acgcatcaca gatgcgctgg ttgaacgctt ttctcattct
    35101 acgctgcaaa tcttgctggt gaatcacatt aaccatgcca atgaggtaga tgaaacattc
    35161 cgtcaggcga tggctaagtt gcgccgggta ggcgttactt tgctcaacca gagcgttctg
    35221 ttacgtggtg tgaacgataa cgcgcaaacg ctggcaaacc tgagtaatgc gttgttcgat
    35281 gcgggcgtga tgccgtatta cctgcatgtg ctcgataaag tacagggcgc ggcgcatttt
    35341 atggtgagtg atgacgaagc acggcagatt atgcgtgagt tgctgacact ggtgtcggga
    35401 tatctggtgc ctaaactggc gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat
    35461 ctccagctac gccagcagta actgattaaa cagggaagtt taatctttgc acgttgcata
    35521 acctttaatt attcatatac attgctgtta ttacttttta tataaccaac accaataatt
    35581 aacctttctt ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat
    35641 tactaccaag cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg
    35701 ttagggagaa cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag
    35761 agcaataatt tcattgcact ggcgctgaaa attaaagagc ctcgcaataa agaatcgttg
    35821 ttctttctgt ctgtcatgga actgcgcgac cttctgattg cgctggaaag ccgtctgcat
    35881 caaaagcata aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc
    35941 attaagaaaa tggtagaaaa tatcccagaa attctggttg atgagcttaa aaatgccgat
    36001 attaatcgtc gggttaacac gctggaactt accgataatc agggcgaaaa tcttaccttc
    36061 gtattaacac tacatgacgg tagcaaatgt gagttagtca tcaatgaatt acagattgaa
    36121 atgctggcgc gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta
    36181 cgtattactt cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc
    36241 aatctggaat atgacaccta ttcccaaccg gaatggaaac ataacttgtt caaccattat
    36301 ctggcggtgc tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg
    36361 gtgaaaacgc gtgaagcaac gccgggtaaa gaagtggagg cgatcactcg ccgaatgctt
    36421 gatttcagcc cccgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc
    36481 gtagcggcaa ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac
    36541 ttacgtgttc aatccaccag caaaactgca ccacaggcga aataatctca tctgttttac
    36601 tacgaggctg gaagtatcca gcctctggat cacatcagtt cgggcactta taaacctgac
    36661 cgataatctg gctatccgtc gggacaaaac tggacaacat tccctgcgac gggctgctga
    36721 tgccataaat cacgttaccg cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc
    36781 cgcgcataga accgccctct tctccgtgtt gcccggaaag ccagttgctt tgcttacctg
    36841 tcgcagtacc aatcagctgg cactctgcgc ctggctgctc gtccacaatg cgtacactct
    36901 gaccggcagc actcaattcg ttgctggagc tacaacccgc catcagtagc gcggcaccga
    36961 caatccctgc taagtatttt acgtgcatgt tattccccat aatcaatgag ctggacgcca
    37021 ctcgcgtcgt ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga
    37081 catttctgcc cgggggtttg tttatttctg cgaggtgcag ggcaattaca tcatgccgcc
    37141 catgccaccc atgccgccca taccgccagc agcgcctaag tcagctgcat cgtttttcgg
    37201 caggtcggta accatgcatt cggtggtgat catcaggcca gccacagaag ctgcgtactg
    37261 cagagcagaa cgagttactt tggttggatc caggataccc atgtcgatca tgttgccgta
    37321 ttcttcggtt gctgcgttgt aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac
    37381 aacagacggt tcttcgccgc agttcaatac gatctgacgc agcggagctt ccattgcacg
    37441 cagtgcaact ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt
    37501 agacgctacg cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc
    37561 acgggtcgcg tgcagggcat cttcaacgcg tgcttttttc tctttcattt caacttcggt
    37621 agcagcaccc actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gctcctgcag
    37681 tttttcacgg tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg
    37741 gccctggatt gcagcttctt cacccacgcc atcaatgatg gtggtggtgt ctttgttgat
    37801 cacaacacgt ttagcctgac ccaggtcttc cagggttgct ttttccagct ccataccgat
    37861 ctcttcagag attacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg
    37921 acgatcgccg aagcccggag ctttaaccgc agcaactttc acgatgccac gcatggtgtt
    37981 aacaaccaga gttgccagcg cttcgccttc tacatcttca gcgatgatca gcagcggttt
    38041 gcctgctttg gcaacagctt ccagaaccgg cagcatttcg cggatgttgg agattttctt
    38101 gtcagccagc aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat
    38161 aaagtaagga gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc
    38221 ctgcagaccg gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc
    38281 ttcagcgatc agtttaccta cggtttcgtc ggagttagcg gagatggtac caacctgagc
    38341 aatcgcttta gagtcagagc atggtacgga cagcgctttc agttcttcaa ctgcagcggt
    38401 aaccgctttg tcgataccac gtttcaggtc catcgggttc atgcccgcag caacagcttt
    38461 cagaccttca gtgatgatag cctgagccag tacggttgca gtggtggtac cgtcgcctgc
    38521 agcgtcgttc gctttggagg caacttcttt caccatctgc gcacccatgt tttcgaactt
    38581 gtcttccagt tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc
    38641 gaaagattta tccagaacta cgttacggcc tttcggaccg agggtaactt tcactgcatc
    38701 tgccagtacg tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc
    38761 tttagctgcc attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gcggattacg
    38821 cttcaacaat tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct
    38881 tctcggattt cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacgtcca
    38941 gcggcttcac ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc
    39001 gggtggattt agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa
    39061 cttctttacg cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc
    39121 ctttgagaaa gtccgtatct gttatgggtg acgccggagc ttacgtggtt tcccggctgg
    39181 tgaccagaga aatggggatg aggcttcgcc ccttcaaggg ggaaaaagaa aaaaaattct
    39241 gatcagcaca aaatcgggtg aaaaccctga ttcacctcac atttcatcgc aatttcttca
    39301 tcgccgtcat aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat
    39361 ttgggcgcaa tcggacatca acccatgagt ggactcaaac aagaactggg gctggcccag
    39421 ggcattggcc tgctatcgac gtcattatta ggcactggcg tgtttgccgt tcctgcgtta
    39481 gctgcactgg tagcgggcaa taacagcctg tgggcgtggc ccgttttgat tatcttagtg
    39541 ttcccgattg cgattgtgtt tgcgattctg ggtcgccact atcccagcgc aggcggcgtc
    39601 gcgcacttcg tcggtatggc gtttggttcg cggcttgagc gagtcaccgg ctggctgttt
    39661 ttatcggtca ttcccgtggg tttgcctgcc gcactacaaa ttgccgccgg gttcggccag
    39721 gcaatgtttg gctggcatag ctggcaactg ttgttggcag aactcggtac gctggcgttg
    39781 gtgtggtata tcggtactcg cggtgccagt tccagtgcaa atctacaaac cgttattgcc
    39841 ggacttatcg tcgcgctgat tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat
    39901 atccccttcc cggcacctgg taatatcgaa cttaccgggt tatttgctgc gttatcagtg
    39961 atgttctggt gttttgtcgg tctggaggca tttgcccatc tcgcctcgga atttaaaaat
    40021 ccagagcgtg attttcctcg tgctttgatg attggtctgc tgctggcagg attagtctac
    40081 tggggatgta cggtagtcgt cttacacttc gacgcctatg gtgaaaaaat ggcggcggca
    40141 gcatcgcttc caaaaattgt agtgcagttg ttcggtgtag gagcgttatg gattgcctgc
    40201 gtgattggct atctggcctg ctttgccagt ctcaacattt atatacagag cttcgcccgc
    40261 ctggtctggt cgcaggcgca acataatcct gaccactacc tgtcacgcct ctcttctcgc
    40321 catatcccga ataatgccct caatgcggtg ctcggctgct gcgtggtgag cactttggtg
    40381 attcatgctt tagagatcaa tctggacgct cttattattt atgccaatgg catctttatt
    40441 atgatttatc tgttatgcat gctggcaggc tgtaaattat tgcaaggacg ttatcgacta
    40501 ctggcggtgg ttggcgggct gttatgcgtt ctgttactga caatggtcgg ctggaaaagt
    40561 ctctatgcgc tgatcatgct ggcggggtta tggctgtttc tgccaaaacg aaaaacgccg
    40621 gaaaatggca taaccacata atccggcgtt tcgacattaa tcctggcgat cgtctttatg
    40681 atcaaggcgg tcgcggtcat catcctttcg ctggtactca ccatcaaaag tattaccgcc
    40741 accggtcccg gcgctaaaac cgccgcctgg catgcgagaa aagcgcaaat gcggcatcaa
    40801 cttcactgtc agatgctttt gcaccggcgg caacaaaagc agaagaccga ggaagtcggt
    40861 gaaaaagccc ggtaataaaa gcagcaaacc ggcaatgatc agcgaaacac ttttaatcat
    40921 ctccgccgct gggttttcac ccgccgccat tttttgctgc atcagcacga aattcttaaa
    40981 gccctggtta cgtaccagtg acataccgat aactgacgtg aatataacca gcacgagagt
    41041 cagcaatacc cccaatacat gggcaacctg aataaagatt gaaatctcaa tatagacata
    41101 aaggaaaatg gcaataaaag gtaaccagcg caaaggtttc tcctgtaata gcagccggtt
    41161 aaacccggct acctgaatgg gttgcgaatc gcgttcagct tatattgtgg tcattagcaa
    41221 aatttcaaga tgtttgcgca actatttttg gtagtaatcc caaagcggtg atctatttca
    41281 caaattaata attaaggggt aaaaaccgac acttaaagtg atccagatta cggtagaaat
    41341 cctcaagcag catatgatct cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa
    41401 aacattcgaa accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag
    41461 aaggttcaca tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt
    41521 ccagctgatg cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc
    41581 aacaacaaaa tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc
    41641 gcagctatgg caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt
    41701 gccgcatgtg atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc
    41761 taccagggcg gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc
    41821 ggtctggaac tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat
    41881 gttaacaaat gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac
    41941 tcttccctga ttaagctggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa
    42001 gctgtcgaat tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg
    42061 atgaccctcg gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaac
    42121 atccaacgta ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact
    42181 ggtctgaaca cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact
    42241 ggcttcccat gcgtaccggc tgaagacctg atcgaagcga cctctgactg cggcgcttat
    42301 gttatggttc acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac
    42361 ctgcgcttgc tctcttcagg cccacgtgcc ggcctgaacg agatcaacct gccggaactg
    42421 caggcgggct cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac
    42481 caggtatgct tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt
    42541 cagctgcagt tgaacgttat ggagccggtc attggccagg ccatgttcga atccgttcac
    42601 attctgacca acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac
    42661 aaagaagtgt gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg
    42721 ttcatcggtc accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt
    42781 gtacgtgaag tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc
    42841 tccgtacaga atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa
    42901 cagtaatcgt acagggtagt acaaataaaa aaggcacgtc agatgacgtg ccttttttct
    42961 tgtgagcagt aacttaaaaa taacaatcta atatcaactt gttaaaaaaa caaggaaggc
    43021 taatatgcta gttgtagaac tcatcatagt tttgctggcg atcttcttgg gcgccagatt
    43081 ggggggaata ggtattggtt ttgcaggcgg attgggggtg ctggttcttg ccgctattgg
    43141 cgttaaaccc ggtaacatcc cgttcgatgt catttccatt atcatggcgg ttatcgccgc
    43201 tatttctgcc atgcaggttg ctggcggtct ggactatctg gttcatcaga cagaaaagct
    43261 gctgcgccgt aacccgaaat acatcacgat cctcgcaccg atcgtgacct atttcctgac
    43321 aatctttgct ggtactggca acatctctct ggcgacactg ccagttatcg ctgaagttgc
    43381 gaaggaacaa ggcgttaaac cttgccgtcc gctgtctact gcagtggtat ccgcgcagat
    43441 tgcgatcacc gcatcgccaa tctcagcggc agtggtttac atgtcttccg taatggaagg
    43501 tcatggcatc agctacctcc atctgctctc cgtggtcatc ccgtccaccc tgctggcggt
    43561 tctggtgatg tccttcctgg tcactatgct gttcaactcc aaactctctg acgatccgat
    43621 ttatcgcaag cgtctggaag aaggcctggt tgaactgcgc ggtgaaaagc agattgaaat
    43681 caaatccggt gcaaaaacgt ccgtctggct gttcctgctg ggcgtagttg gcgttgttat
    43741 ctatgcaatc atcaacagcc caagcatggg tctggttgaa aaaccgctga tgaacaccac
    43801 caacgcaatc ctgatcatca tgctcagcgt tgcaactctg accaccgtta tctgtaaagt
    43861 cgataccgac aacatcctca actccagcac cttcaaagca ggtatgagcg cctgtatttg
    43921 tatcctgggt gttgcgtggc tgggcgatac tttcgtttcc aacaacatcg actggatcaa
    43981 agataccgct ggtgaagtga ttcagggtca tccgtggctg ctggccgtca tcttcttctt
    44041 tgcttctgct ctgctgtact ctcaggctgc aaccgcaaaa gcactgatgc cgatggctct
    44101 ggcactgaac gtttctccgc tgaccgctgt tgcttctttc gctgcggtgt ctggtctgtt
    44161 cattctgccg acctacccga cgctggttgc tgcggtacag atggatgaca cgggtactac
    44221 ccgtatcggt aaattcgtct tcaaccatcc gttcttcatc ccgggtactc tgggtgttgc
    44281 cctggccgtt tgcttcggct tcgtgctggg tagcttcatg ctgtaatgat caatcgcggg
    44341 gcgttcacgc cccgctttct ttcccgccga ctaacatcct tcccccgtcc gttgtatagt
    44401 gacctctctc ttgcggttcc atctgttctt gcgaggtgtt tatgcttgat gaaaaaagtt
    44461 cgaataccgc gtctgtcgtg gtgctatgta ccgcaccgga tgaagcgaca gcccaggatt
    44521 tagccgccaa agtgctggcg gaaaaactgg cggcctgcgc gaccttgatc cccggcgcta
    44581 cttctctcta ttactgggaa ggtaagctgg agcaagaata cgaagtgcag atgattttaa
    44641 aaactaccgt atctcaccag caggcactgc tggaatgcct gaagtctcat catccatatc
    44701 aaaccccgga acttctggtt ttacctgtta cacacggaga cacagattac ctctcatggc
    44761 tcaacgcatc tttacgctga tcctgctact ttgcagcact tccgtttttg ccggattatt
    44821 cgacgcgccg ggacgttcac aatttgtccc cgcggatcaa gcctttgctt ttgattttca
    44881 gcaaaaccaa catgacctga atctgacctg gcagatcaaa gacggttatt acctctaccg
    44941 taaacagatc cgcattacgc cggaacacgc gaaaattgcc aacgtgcagc tgccacaagg
    45001 cgtctggcat gaagatgagt tttacggcaa aagcgagatt taccgcgatc ggctgacgct
    45061 tcccgtaacc atcaaccagg cgagtgcggg agcgacgtta actgtcacct accagggctg
    45121 tgctgatgcc ggtttctgtt atccgccaga aaccaaaacc gttccgttaa gcgaagtggt
    45181 cgccaacaac gcagcgtcac agcctgtgtc tgttccgcag caagagcagc ccaccgcgca
    45241 attgcccttt tccgcgctct gggcgttgtt gatcggtatt ggtatcgcct ttacgccatg
    45301 cgtgctgcca atgtacccac tgatttctgg tatcgtgctg ggtggtaaac agcggctctc
    45361 cactgccaga gcattgttgc tgacctttat ttatgtgcag gggatggcac tgacttacac
    45421 ggcgctgggt ctggtagttg ccgccgcagg gttacagttc caggcggcgc tacagcaccc
    45481 atacgtgctc attggcctcg ccatcgtctt taccttgctg gcgatgtcaa tgtttggttt
    45541 gtttaccctg caactcccct cttcgctgca aacgcgcctc acgctgatga gcaatcgcca
    45601 acagggcggc tcacctggcg gtgtgtttgt tatgggagcg attgccggac tgatctgttc
    45661 accatgcacc accgcaccgc ttagcgcgat tctgttgtat atcgcccaaa gcgggaacat
    45721 gtggctgggg ggcggcacgc tttatctcta tgcgttgggc atgggcctgc cgctgatgct
    45781 aataaccgtc tttggtaacc gcctgctgcc gaaaagcggc ccgtggatgg aacaagtcaa
    45841 aaccgcgttt ggttttgtga tcctcgcact gccggtcttc ctgctggagc gagtgattgg
    45901 cgatatatgg ggattacgct tgtggtcggc gcttggtgtc gcattctttg gctgggcctt
    45961 tatcaccagc ctacaggcca aacgcggctg gatgcgcgtg gtgcaaataa tcctgctggc
    46021 agcggcattg gttagcgtgc gcccacttca ggattgggca tttggtgcaa cacataccgc
    46081 gcaaactcag acgcatctca actttacaca aatcaaaacg gtagatgagt taaatcaggc
    46141 gctcgttgaa gccaaaggca aaccagtgat gttagatctc tatgccgact ggtgcgtcgc
    46201 ctgtaaagag tttgagaaat acaccttcag cgacccgcag gtgcaaaaag cgttagcaga
    46261 cacggtctta cttcaggcta acgtaactgc caacgacgca caagatgtgg cgctgttaaa
    46321 gcatcttaat gtccttggcc taccgacaat tctctttttt gacggacaag gccaggagca
    46381 tccacaagca cgcgtcacgg gctttatgga tgctgaaacc ttcagcgcac atttgcgcga
    46441 tcgccaaccg tgaacgacac ttgcagtggg aaagacggag gaaaatagcg tgcaacgtga
    46501 agatgtactg ggagaagccc tgaaattatt agaattacaa gggattgcca acaccacgct
    46561 tgagatggtt gctgaacgtg tggattaccc actggacgag ctacgccgct tctggccaga
    46621 caaagaggca atcctctacg atgcgctgcg ttatcttagc caacagatag atgcctggcg
    46681 tcgtcagctg atgctggacg aaacgcaaac cgccgaacaa aagctgctgg cacgttatca
    46741 ggcgttatcg gagtgcgtta aaaacaaccg ctatccgggc tgtctattta tcgctgcctg
    46801 tacgttttat cccgatcctg gccaccctat tcatcaactg gccgatcagc aaaaaagcgc
    46861 ggcctacgat ttcacccacg aactgttaac cacactggaa gttgacgatc cggcgatggt
    46921 agcaaagcag atggaactgg tgctggaagg ctgtttaagc cgaatgctgg tgaatcgtag
    46981 ccaggcggat gtcgacaccg cacatcggct ggcggaagat atcctgcgct tcgcccgctg
    47041 ccgccagggt ggtgctttga cctgacagaa acacagaaaa gaagcgattt gccgcaatct
    47101 taagcagttg aatcgctttt actgaaatta ggttgacgag atgtgcagat tacggtttaa
    47161 tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcg
    47221 gtggttcgat tccgcctccg ggcaccatat accagaacac gctggttaag ggatgagatc
    47281 cttgacgggc ggttaataca ggatgcggca tcaaagggtc tccgtttgtg taagttgtgg
    47341 ttacactgat tgagttcggt atcgtatcct caaacagggt gtcttttgcg gagacgccct
    47401 gttctcgtta acactgtatg cttacatcgc tgattcagcc tgtcaagatt tattcccccc
    47461 tttgcaaatc ttgctcactg tgcgctcatc tgtatacaaa ctcagggcat cttccagtgt
    47521 ctg
//
LOCUS       DAZKJR010000021        47212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKJR010000021 DAZKJR010000000
VERSION     DAZKJR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.021"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(70..636)
                     /gene="mepS"
                     /locus_tag="ACHOWS_001220"
     CDS             complement(70..636)
                     /gene="mepS"
                     /locus_tag="ACHOWS_001220"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFQ0046835.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(1048..1761)
                     /gene="lpxT"
                     /locus_tag="ACHOWS_001221"
     CDS             complement(1048..1761)
                     /gene="lpxT"
                     /locus_tag="ACHOWS_001221"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFQ0046836.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(1800..2786)
                     /gene="yeiR"
                     /locus_tag="ACHOWS_001222"
     CDS             complement(1800..2786)
                     /gene="yeiR"
                     /locus_tag="ACHOWS_001222"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFQ0046837.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHGVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(2904..4370)
                     /gene="yeiQ"
                     /locus_tag="ACHOWS_001223"
     CDS             complement(2904..4370)
                     /gene="yeiQ"
                     /locus_tag="ACHOWS_001223"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0046838.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARALMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNTIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            complement(4593..5165)
                     /gene="yeiP"
                     /locus_tag="ACHOWS_001224"
     CDS             complement(4593..5165)
                     /gene="yeiP"
                     /locus_tag="ACHOWS_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFQ0046839.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            5320..5574
                     /gene="yeiW"
                     /locus_tag="ACHOWS_001225"
     CDS             5320..5574
                     /gene="yeiW"
                     /locus_tag="ACHOWS_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFQ0046840.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(5571..6752)
                     /gene="setB"
                     /locus_tag="ACHOWS_001226"
     CDS             complement(5571..6752)
                     /gene="setB"
                     /locus_tag="ACHOWS_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFQ0046841.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVTVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            7120..8250
                     /gene="fruB"
                     /locus_tag="ACHOWS_001227"
     CDS             7120..8250
                     /gene="fruB"
                     /locus_tag="ACHOWS_001227"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFQ0046842.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            8250..9188
                     /gene="fruK"
                     /locus_tag="ACHOWS_001228"
     CDS             8250..9188
                     /gene="fruK"
                     /locus_tag="ACHOWS_001228"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0046843.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFDVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            9205..10896
                     /gene="fruA"
                     /locus_tag="ACHOWS_001229"
     CDS             9205..10896
                     /gene="fruA"
                     /locus_tag="ACHOWS_001229"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFQ0046844.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVATSG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYISFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            11320..12261
                     /gene="psuK"
                     /locus_tag="ACHOWS_001230"
     CDS             11320..12261
                     /gene="psuK"
                     /locus_tag="ACHOWS_001230"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFQ0046845.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            12249..13187
                     /gene="psuG"
                     /locus_tag="ACHOWS_001231"
     CDS             12249..13187
                     /gene="psuG"
                     /locus_tag="ACHOWS_001231"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFQ0046846.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            13281..14531
                     /gene="psuT"
                     /locus_tag="ACHOWS_001232"
     CDS             13281..14531
                     /gene="psuT"
                     /locus_tag="ACHOWS_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFQ0046847.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(14599..15297)
                     /gene="yeiL"
                     /locus_tag="ACHOWS_001233"
     CDS             complement(14599..15297)
                     /gene="yeiL"
                     /locus_tag="ACHOWS_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFQ0046848.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            15427..16368
                     /gene="rihB"
                     /locus_tag="ACHOWS_001234"
     CDS             15427..16368
                     /gene="rihB"
                     /locus_tag="ACHOWS_001234"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFQ0046849.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            16468..17718
                     /gene="nupX"
                     /locus_tag="ACHOWS_001235"
     CDS             16468..17718
                     /gene="nupX"
                     /locus_tag="ACHOWS_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFQ0046850.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKSFINRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(17774..18862)
                     /gene="yeiI"
                     /locus_tag="ACHOWS_001236"
     CDS             complement(17774..18862)
                     /gene="yeiI"
                     /locus_tag="ACHOWS_001236"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFQ0046851.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(18865..19722)
                     /gene="nfo"
                     /locus_tag="ACHOWS_001237"
     CDS             complement(18865..19722)
                     /gene="nfo"
                     /locus_tag="ACHOWS_001237"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0046852.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(19796..20845)
                     /gene="yeiH"
                     /locus_tag="ACHOWS_001238"
     CDS             complement(19796..20845)
                     /gene="yeiH"
                     /locus_tag="ACHOWS_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFQ0046853.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            20944..21825
                     /gene="yieE"
                     /locus_tag="ACHOWS_001239"
     CDS             20944..21825
                     /gene="yieE"
                     /locus_tag="ACHOWS_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFQ0046854.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            22030..23499
                     /gene="lysP"
                     /locus_tag="ACHOWS_001240"
     CDS             22030..23499
                     /gene="lysP"
                     /locus_tag="ACHOWS_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFQ0046855.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAVS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            23792..25771
                     /gene="cirA"
                     /locus_tag="ACHOWS_001241"
                     /pseudo
     CDS             23792..25771
                     /gene="cirA"
                     /locus_tag="ACHOWS_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(25803..26639)
                     /gene="yeiG"
                     /locus_tag="ACHOWS_001242"
     CDS             complement(25803..26639)
                     /gene="yeiG"
                     /locus_tag="ACHOWS_001242"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFQ0046856.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            26897..27565
                     /gene="folE"
                     /locus_tag="ACHOWS_001243"
     CDS             26897..27565
                     /gene="folE"
                     /locus_tag="ACHOWS_001243"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFQ0046857.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            27582..28739
                     /gene="yeiB"
                     /locus_tag="ACHOWS_001244"
     CDS             27582..28739
                     /gene="yeiB"
                     /locus_tag="ACHOWS_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFQ0046858.1"
                     /translation="MGRNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPARSKTSR"
     gene            28881..29921
                     /gene="galS"
                     /locus_tag="ACHOWS_001245"
     CDS             28881..29921
                     /gene="galS"
                     /locus_tag="ACHOWS_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFQ0046859.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            30201..31199
                     /gene="mglB"
                     /locus_tag="ACHOWS_001246"
     CDS             30201..31199
                     /gene="mglB"
                     /locus_tag="ACHOWS_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFQ0046860.1"
                     /translation="MNKKVLTLFAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            31260..32780
                     /gene="mglA"
                     /locus_tag="ACHOWS_001247"
     CDS             31260..32780
                     /gene="mglA"
                     /locus_tag="ACHOWS_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFQ0046861.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            32796..33806
                     /gene="mglC"
                     /locus_tag="ACHOWS_001248"
     CDS             32796..33806
                     /gene="mglC"
                     /locus_tag="ACHOWS_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFQ0046862.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(33877..35112)
                     /gene="preA"
                     /locus_tag="ACHOWS_001249"
     CDS             complement(33877..35112)
                     /gene="preA"
                     /locus_tag="ACHOWS_001249"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFQ0046863.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(35106..36344)
                     /gene="preT"
                     /locus_tag="ACHOWS_001250"
     CDS             complement(35106..36344)
                     /gene="preT"
                     /locus_tag="ACHOWS_001250"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFQ0046864.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLKGACSC"
     gene            complement(36663..36902)
                     /gene="yeiS"
                     /locus_tag="ACHOWS_001251"
     CDS             complement(36663..36902)
                     /gene="yeiS"
                     /locus_tag="ACHOWS_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFQ0046865.1"
                     /translation="MDVQQFFVVAVFFLIPVFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(36905..37624)
                     /gene="sanA"
                     /locus_tag="ACHOWS_001252"
     CDS             complement(36905..37624)
                     /gene="sanA"
                     /locus_tag="ACHOWS_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFQ0046866.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(37774..38658)
                     /gene="cdd"
                     /locus_tag="ACHOWS_001253"
     CDS             complement(37774..38658)
                     /gene="cdd"
                     /locus_tag="ACHOWS_001253"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0046867.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALVAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(38788..39483)
                     /gene="yohK"
                     /locus_tag="ACHOWS_001254"
     CDS             complement(38788..39483)
                     /gene="yohK"
                     /locus_tag="ACHOWS_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFQ0046868.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(39480..39878)
                     /gene="yohJ"
                     /locus_tag="ACHOWS_001255"
     CDS             complement(39480..39878)
                     /gene="yohJ"
                     /locus_tag="ACHOWS_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0046869.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            complement(40009..40917)
                     /locus_tag="ACHOWS_001256"
     CDS             complement(40009..40917)
                     /locus_tag="ACHOWS_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0046870.1"
                     /translation="MANWAQKLKLHHLQMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGLTLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLKEMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYRVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            41044..42402
                     /locus_tag="ACHOWS_001257"
     CDS             41044..42402
                     /locus_tag="ACHOWS_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0046871.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAIIRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAVCIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTQLITVVRQSTSVPDSLPHAGVV
                     NE"
     gene            42414..43442
                     /gene="gtdA"
                     /locus_tag="ACHOWS_001258"
     CDS             42414..43442
                     /gene="gtdA"
                     /locus_tag="ACHOWS_001258"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFQ0046872.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTSMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVTIGNETFSFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            43458..44159
                     /locus_tag="ACHOWS_001259"
     CDS             43458..44159
                     /locus_tag="ACHOWS_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFQ0046873.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAIVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEN
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKASETGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGSVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            44167..44811
                     /gene="maiA"
                     /locus_tag="ACHOWS_001260"
     CDS             44167..44811
                     /gene="maiA"
                     /locus_tag="ACHOWS_001260"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFQ0046874.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLESHYPQVPLMPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            44826..46019
                     /locus_tag="ACHOWS_001261"
     CDS             44826..46019
                     /locus_tag="ACHOWS_001261"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFQ0046875.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARQGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEIARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWIGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPCCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            46081..47028
                     /gene="dusC"
                     /locus_tag="ACHOWS_001262"
     CDS             46081..47028
                     /gene="dusC"
                     /locus_tag="ACHOWS_001262"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFQ0046876.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPAGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT        11492 a        11710 c        12482 g        11528 t
ORIGIN      
        1 aagcactgcc taagcagtgc tttcagttaa ctcgtcagga tagccaaggg attgcatcca
       61 aacggtttat tagctgcggc tgagaacccg gcgtgcttcg ttgtaacgct tcttccagta
      121 cggttcattc atgctggaaa taataacacc actgctggtg gaagcatgga caaactgatt
      181 gttgccgata taaataccga catggcgtcc tgttgaaccg gcacggaaca gaactaaatc
      241 acccgtacgc aaattactac gggaaacaga tttacccatt tcctgctgtt cgtaagtcga
      301 acgcggaagt tctaagccaa attgctcacg gaatgtacgc tgtacgaaac cagaacaatc
      361 gatgcctttt ttagtgctgc cacccagacg atagcgtaca cctttccagt cagcatactg
      421 atccataatt cgcgatttta cgtcgacatt acgaaccagg ttttcaaatt catcctgaga
      481 agcttgcagt gatgatgttt cactacccac tgcacgtgtc tcaggatgca tattctttgc
      541 ggtgttattt gcactacatg cagaaagcag aaccgctact gcaatcgcgg ggatcccgcg
      601 caagatatat ctcaaaatcg gttgagattt gaccatgttg tttgttttcc cttgaagtcc
      661 ttaacgacaa atatcgttat aaaaaaatgc caagcgacac gagactaatt acctgggcat
      721 gttgaaacaa ttccttaagg ccaaatctgt ggactaacgc acaaaattat atgctgtgag
      781 gataatctac cgccgaggca aaacgagttc gagattaccc gattggtgcg gtcttggcga
      841 gcggttttac atgatttttt ataagaaaaa gtgatacaga aagttaataa acgggattgg
      901 tcaattaaag tacgattgag tgaaacgcat gcaatccatt catttactta gagaatgatt
      961 tttgcgcgcg atggatgaaa gcacggtgcg catcatcagg attatcctca ctataaaaat
     1021 aaccctgatg atgttaatta ctgtgagtta tttgttttgg aaatgtttgt tttttcccgg
     1081 tagtgatttg tcaaaaaagg tgattaatcg atcacttaat ggcgtcagca acacccaggg
     1141 caaaccgatc aatatcacgg tcatcgaacc gacaatgatg tcagtaaacc agtgtgcgcc
     1201 aatcattact ctgggaaacg caaaaaccac aaaaataata agggcgataa ggcctgcaac
     1261 tttgccgaaa taacgccaca tgaatgccga aaaaataagc agcatcatac cgtgatcgcc
     1321 gggaaagcta tctcgtgagg catctttcgt gggaacagag agcagttcgc tgacgcggtt
     1381 aatatcggta aaagtcaatg ttgggctggc ccgttttaca ggaattaatg cctgacccag
     1441 ctggtttaat accactgcag ttaatagcat gaccagacca ataatcacga tacgtcgtct
     1501 gcctggggcg ttttctttca gccagaaact cagcatcaac attcccatcg ccagcagtga
     1561 acaaccgtcg aaagcgcgat tgttggtcaa tgcaaccagc cacaaaaagg ccttactttc
     1621 gaccagtttc tgattaaaaa aataaaaaat atccgcatca atcggcaacc agaatccatg
     1681 attaacggga atataccagg aaagaaacag cgcgaggccg acaatattca acaacactat
     1741 ttgcggcaaa tttttaatca tattttctct aattatctta ctgaaaaacg caggcaacct
     1801 tacgcggtgg tcgctaaacg aagcttcaac agcgcgctct gcaaggcatt ccagtcagct
     1861 tcgctgctgg aaatcagctc aatacgactg tccggtggcg caacgttttg cgtttcaatg
     1921 tgcaggtcat cgccctgacg gttgattcgc accagccctt cgggaatacg catcacgcct
     1981 ttgacgcgtt ccaccggtgc aagtcgcgcc cattccagaa tgccaatggt gtcgaatacc
     2041 gtatcagcgt cgaatatcca gccacaggcc tgatatcctt gcccgctgtt cagactgcga
     2101 cgccagcgtt gatgctctgg caggcttaac gctgctaacc cttttttcac gccatgctga
     2161 tgagaatgcg cggcgctggc gggcaactcg gctaaattgc gacgcggcaa atccagaaga
     2221 tgaccgtcaa ttttcccatg ctcgctgtga attaattgcc gatcgccacc attttgctgc
     2281 caccagcgct gtagcgcttg ctcactttcg ggcgtcgcac ggtcggattt attggcgaca
     2341 ataatgtctg cggcagccag ctggtcacgg aagttttcat tgctggcgct tttttcgtcc
     2401 agcagcaggc gtggatcgag aatgcacaac gtggcgcgca gatctatcca cggttcatac
     2461 acgggcgcgg ttaagagatc taggatctgt ttcggatgac ccagtccggt cggctctatc
     2521 aacaagcggt ctggttttcc ctgacgcagt aaggtattca atcctacctg catgggtaaa
     2581 ccattaacgc agcacatgca gccgccgggg atctctttca gcaatgcgcc gctatcggcg
     2641 agcaaagcac catcaattcc gacttcccca aattcattaa ccagtaccgc ccatttttcg
     2701 ttgggatctt tatgggctaa cagatgaaga atcgacgtgg ttttcccgct gccgagaaaa
     2761 ccggtgatga ggttggtcct ggtcacaacg gctccgcaat actcttaaaa gttaactgag
     2821 cgttaatcct ggctaaaaac ggcagaaaag agaaggggag agcggacgaa ggcgagcttc
     2881 atccgcttta accgcagaaa tcgttaaatt ttgagggtgt ttaatagcgc ctgatgcgcg
     2941 ccgaattgtg cgatttgttg ccaggcttgt tcgatggcct gcacaaaatg cgggttatct
     3001 ggcagatcat caccgaaaac ttcacgcagg gaaagcaggg cggtgacgcg ttgctcactg
     3061 ctgctgcccg caacaagatc gcgaatttta tcgctaagcg gatcgcgaac atcaatggta
     3121 tttccggcat catcaacgcc gctgacgtaa cgcatccagc ctgcaacgcc taatgccagc
     3181 aacgaccagt ccgtttcgcg cgccagatgt atgcgaatac ctgccagcat acgttgcggt
     3241 aatttctggc taccatccat tgcgatttgc caggtcttat gtttcagcgc cgggttagca
     3301 aaacgtgcaa ttaacttatc cgcatattgt gttaaatcga catctttaat tcgcagtgtc
     3361 ggcgcttgct catccagcat taatgctctg gcggcatggc gaaatgcgcg atcctgcatg
     3421 caatcactga tatgggcgaa tcctgagagg taacccagat aagcgagaaa agagtggctg
     3481 ccattaagca tacgcagttt catctcttcc catggcagga catcattcac catttgtaca
     3541 cctgcgactt cccaggcagg acgcccagcg acgaagttat cttccaccac ccactggata
     3601 aacggttcgc agctaatcgc gcagggatca tttaccccca gatgctggct gatttccgcc
     3661 agtgattcgt cggttgctgc cggaacaatg cggtcgacca tagttcccgg aaaactgacg
     3721 tgctctttta tccacccggc gagttctggc gaacgttttt ctgccattcc cagcaccgcg
     3781 tttttcacca catggccatt gtcgggaata ttgtcgcagg agagcacggt aaacggtgta
     3841 aggccgcgct cacggcggcg tttcagtgct tcgacgagaa tacccggtgc ggagtgaggt
     3901 tcttcagggg tttgtagatc gtgaataatc cgcggattgc tggtgtcgag tgcaccggtg
     3961 gccgggtcaa tacaatagcc tttttcggta atcgtcaggg aaacaattgc cacctgtggc
     4021 tcgcaaaatt tctcaataat tgccgctaag gaatccagtt ttgcattcag gcattcgtga
     4081 acggcaccga caattatcgc ctgattgccg tccgcccctt tctccagcac ggtgtataaa
     4141 tggttctgtg cgcggagctg gctcatcagt tgatcaccgc tgaacaagct gatttcacag
     4201 atcccccagt cgccgctctg ggcattcagc acacgatcgg tcagtaacgc ctgatgtgcg
     4261 cggtgaaagg ctccaaagcc aaaatgaacg atacgtgatt gcaactgctg gcgatcgtag
     4321 cgtggagcgt gtacatgatg cgggagcgta acggaggcga ttgtattcat aggagtcacg
     4381 tcttagtaac gattaattaa caatgacagt gtaaagttat atgacagcaa ttgaaattgg
     4441 tctggattgt taatgcgatg aagtgtgagg gcgatcaatc aacgagtgaa aattgcgggc
     4501 gtaaggtgtt caggaagcgc aaaatagaag attggtataa taaattaaaa aagggacagc
     4561 ctgagctatc ccttttctgc atgcggctga agttagtcag cacgccccat ataacggcgt
     4621 tcttcgatat ggatacgaat tttttcgccc gggcttaagt attctggtac ctgaatcacc
     4681 agaccagtgc tcaatgtcgc cggtttgtta cgggcgctgg cagatgcccc tttgatgcct
     4741 ggtgcggttt caacgatttc cagatcaacg gtctgcggca actcaagcgc cagcagttga
     4801 ccgtcccagg tcagcacctg catgtccggc atgccgccct ctggcataaa cagcaactct
     4861 tcttcaatct gatctttggt gaaggtatac ggggtatagt cttctttatc cataaagaca
     4921 tattcgttgc catcgacata ggagaagtca acgtaacggc gggtcagcgt cacggtgtca
     4981 acgatatcat ctcctttgaa acgctcttct actttcagcc cggtacggac atcagaaaaa
     5041 cgcattttgt acagcgtagc ggcaccgcgg gcagtgggcg actgaatatc aatgtcctta
     5101 accagcagca gtttgccgtt gtaattcagt accatacctt ttttaatttc gttcgctctt
     5161 ggcatcgaaa aattcctgta gtcgaaaatg tcaaaaatat cgcgacaaag tactcgcgac
     5221 gggcctttcg ggcaagtggt attcgcactt ttgctggtgc aaaaaaggtg gtactgtgcg
     5281 cgctcatcaa tccggtggtt aaccttaaga gaacaacgta tggaatgccg tccgggttgc
     5341 ggggcgtgtt gcactgcccc ttcgatttcc agccctattc ccggtatgcc agatggcaag
     5401 cccgccaata cgccctgcat tcagcttgat gaacagcaac gctgtaaaat tttcacctcg
     5461 ccactgcggc cgaaagtctg tgcaggctta caagccagcg ctgaaatgtg cggtaactcg
     5521 cgtcagcagg caatgacctg gcttatcgat ctggaaatgc tgaccgcgcc ttaaacatct
     5581 ttaatccgca gtaagcaaaa cagagtggcg ataatcatca ccatcgcaaa ccagaacaca
     5641 gcgtgataat tccagatctc ggcgacgatg cccgccactg atcctgcgat gatccagccc
     5701 acgcgcgacg tattggtata gagcgtggtg gctgaacctg cctgaccggg catcaaatcc
     5761 tgaaaataga gcatcccgat gccacccaga atgccaataa aaatagcatt tagcagctgc
     5821 aagcccaaca gaatgacagg tgaatgcgcc atcagcattc ctgcgtaaaa acagacgcca
     5881 cccacggcag caacgcgcat taagaaacgc ttacccagac gtttggcgaa atatccggca
     5941 atcaacatgg tcgggatttc cagcccggcg gcggtcccca tcatcacacc ggccagtttc
     6001 tcgggaagat gcagttcgtt gataataaat agcggcatgt tgatgatgta caggctgttc
     6061 gagccccaca tcaatgtaca aatgacaaac agcagcagcg tatcgcgacg gttacggcgc
     6121 ggcgcttcaa ccgtgccggt cgccagcgga agttcttttt gcattgacgg taaaaacagc
     6181 cacaccgtca cgccgcaaac aataaacgct accgctgcgc tcagatacat taccgtaaag
     6241 ctgaaaccca tcgctaaggc ataagccagc ggtgggccaa tgacccatgc cagtgaaacc
     6301 tgagcgcgta aaaaagagct gaacatcacc gcctcacgtc cggttttgtc ggcatgttca
     6361 cgggcaaggg caaacatttg cgggttagcg gtcgagccaa agctgctaag aaagacgcca
     6421 acgaatagca aaacaaagta gttgcgattc caggcaaaaa gggtgcaggc cagcacgcct
     6481 aacaggcagc aaaagacaat cagcgatttg cgatcgccgc gcttatcaga gcgcccggcg
     6541 agaaactgac tcaccagaat cccaatgaca gcgctgccgg taaagaagaa tcccaccatc
     6601 gccggacggg catgtacttc atcggtaaga aaaatactga gtgtcggggt ttgcagagcg
     6661 cccgcaatac cggtgagaaa ggcaacgatt aaaaacgccg tcgaggtcag gtcaaacgat
     6721 ttcgcgctgg tgactgcggg ggagttatgc atgctggtcg atcactggaa gaggaagacg
     6781 cggagtttac gctgcttgtc agaaaataaa cagacagcga aatcaaaagc gccacgaata
     6841 atcaaaatgc catttcactg ttgctgattg gctgctgacg ctgctgaaat gaaattgctg
     6901 ataacggatt ttcccatcag caattaggaa aaatgacaaa aaattgtgca gcacatcaaa
     6961 cttttgctca taactttacg gctttccttg cgtgctgaaa caaaagtatt atgctctctt
     7021 gaaacgtttc agcgcgatct tgtctttaac cctaagccag gttggcgctt tttttctcat
     7081 agaagctgaa tcgtttcaat tcagcaagag aggagaacta tgttccagtt atccgtacag
     7141 gacatccatc cgggcgaaaa ggccggagac aaagaagagg cgattcgcca ggtcgctgcg
     7201 gcgctggtgc aggccggtaa tgtagctgaa ggctacgtca atggcatgct ggcgcgcgaa
     7261 cagcaaacct caacgttcct cggcaatggt attgctattc cacacggcac taccgacacc
     7321 cgcgatcagg tgctgaaaac cggcgttcag gtatttcagt tcccggaagg cgtcacctgg
     7381 ggtgacggcc aggtagcgta cgtggcgatc ggtattgctg ccagctcgga tgaacatctg
     7441 ggcctgctac gccagctgac ccacgtactg agcgatgatt ccgttgctga acaactgaag
     7501 tcagcaacaa cagcagaaga acttcgcgca ttgctgatgg gcgaaaagca gagtgagcag
     7561 ctgaagctcg acaacgaaat gctgacgctg gatatcgtcg ccagcgatct gctgactctt
     7621 caggcgctga acgctgcgcg tctgaaagaa gcgggggcag ttgacgccac tttcgtcacc
     7681 aaagccatca atgaacaacc gctgaacctc gggcagggta tctggctgag cgatagcgcc
     7741 gaaggcaatc tgcgtagcgc gattgcggta agccgtgcgg caaatgcttt tgatgtggac
     7801 ggcgaaacgg cagccatgct ggtgagtgtg gcgatgaatg acgatcagcc cattgcggtt
     7861 cttaagcgtc tcgctgattt gttactcgac aataaagctg accgcttgct gaaagcggat
     7921 gcggcaacgt tgctggcgct gctgaccagc gatgatgcgc cgaccgacga cgtgttaagc
     7981 gcggagtttg tggtgcgcaa tgaacacggc ctgcatgctc gtccaggtac catgctggtc
     8041 aataccatta aacaatttaa cagtgatatt accgtgacaa accttgatgg taccggcaaa
     8101 ccggcaaacg gacgtagtct gatgaaagtt gtggcacttg gcgttaagaa aggtcatcgc
     8161 ctacgcttta ccgcccaggg tgcagatgct gaacaggcgc tgaaagcaat cggcgacgct
     8221 atcgctgctg gtcttgggga gggcgcataa tgagcagacg tgttgctact atcaccctta
     8281 atccggctta tgaccttgtt ggtttctgcc cggaaattga acgcggcgaa gtgaacctgg
     8341 tgaaaaccac cggtctgcat gcggcgggta aaggcatcaa cgtggccaaa gtattaaaag
     8401 acctgggaat tgatgtcacc gttggtggct tcctcggtaa agacaatcag gatggttttc
     8461 agcaactgtt cagcgagctg ggcattgcca accgtttcca ggttgtacag gggcgcaccc
     8521 gaattaacgt taagctgacg gaaaaagacg gcgaagtgac cgacttcaac ttctcgggtt
     8581 ttgacgttac ccccgccgac tgggaacgct ttgtgactga ttctctgagc tggctcggtc
     8641 agttcgatat ggtctgtgtc agcggaagct taccgtcagg cgtcagcccg gaagcgttca
     8701 ccgactggat gactcgcctg cgtagtcagt gtccttgcat tatctttgat agtagccgtg
     8761 aagcgttagt agcaggtttg aaagcggcac cgtggctggt gaaacctaac cgccgcgagc
     8821 tggaaatctg ggcaggccgt aaactgcctg aaatgaaaga tgtgattgaa gctgcgcatg
     8881 cgctgcgtga acaaggtatc gcgcatgtcg ttatttcact gggtgccgaa ggcgcgcttt
     8941 gggttaatgc ctccggcgaa tggatcgcca aaccaccgtc agtcgatgtc gtaagcaccg
     9001 ttggcgcagg ggattctatg gttggtggcc tgatttatgg cttgctgatg cgtgaatcca
     9061 gtgaacacac actgcgtctg gcgacagctg ttgcagccct ggcggtaagt caaagcaatg
     9121 tgggtattac cgatcgtccg cagttggccg caatgatggc gcgcgtcgac ttacaacctt
     9181 ttaactgaca gcaggagagg cataatgaaa acgctgctga ttattgacgc taatctcggt
     9241 caggcacgcg cctatatggc gaagaccctg ctgggcgcgg cggcgcgaaa agcaaaactg
     9301 gaaatcatcg acaatccgaa cgacgctgaa atggcgattg ttctcggtga ttccatcccg
     9361 aacgacagcg cgctgaacgg taaaaatgtc tggctgggcg atatttcccg ggcagttgcg
     9421 caccctgagc tgttcctgag tgaagccaaa ggccatgcga aaccttacac tgcaccggtc
     9481 gctgcgacag caccagttgc caccagcggt ccgaaacgcg tagttgcggt gacagcttgc
     9541 ccgactggcg tagcacacac atttatggcg gctgaagcca ttgaaaccga agcgaaaaaa
     9601 cgtggctggt gggtgaaagt tgaaacccgt ggttctgttg gcgcgggtaa tgcgatcacc
     9661 cctgaagaag tagccgcagc ggatctggtg attgtggcgg cagatatcga agtggatctg
     9721 gcgaaatttg ctggtaaacc gatgtatcgc acctctaccg gtctggcgct gaagaaaacc
     9781 gcgcaggaac tggataaagc ggttgctgaa gcaacgccgt atgaaccggc gggcaaagct
     9841 caaacagcga ccactgaagg taagaaagag ggtgcaggcg cttaccgtca cttgctgacg
     9901 ggcgtctctt acatgctgcc gatggtcgtt gcaggtggtc tgtgtatcgc gctttctttt
     9961 gcttttggta tcgaagcgtt taaagagccg ggtacgttgg cagcggcgct gatgcagatt
    10021 ggtggtggtt cagcctttgc gctgatggtg ccggtactgg caggttacat ttccttctcc
    10081 attgccgatc gtccgggtct cacgccgggt ctgattgggg gtatgctggc ggtcagcacc
    10141 ggttctggct tcattggggg tattattgcg ggcttcctgg ctggttacat tgcgaagtta
    10201 atcagtacgc aactgaaact gccacagagt atggaggcgc tgaaaccgat cctgatcatt
    10261 ccgctaattt ccagtctggt ggtcggtctg gcgatgatct acctgatcgg taaaccggtt
    10321 gctggcattc tcgaagggtt gactcactgg ctgcagacca tggggactgc gaatgcggtt
    10381 ctgctggggg cgatcctcgg tggcatgatg tgtactgaca tgggcggtcc ggtaaataaa
    10441 gcagcgtacg cattcggtgt gggtctgctg agtactcaaa cctatggccc gatggcggcg
    10501 attatggcgg caggtatggt gccaccgctg gcaatgggtc tggcaacaat ggtggcgcgt
    10561 cgcaaattcg acaaagcgca gcaggaaggt ggcaaagccg ctctggttct tggtctgtgc
    10621 tttatttcgg aaggtgcaat tccgtttgct gctcgtgatc cgatgcgtgt gctgccgtgt
    10681 tgtatcgttg gtggggcgct gactggcgca atctcaatgg cgattggtgc gaaacttatg
    10741 gcaccgcacg gtggtctgtt tgttctgctg atccctggcg ctattacgcc ggtactgggt
    10801 tacctggtag caattattgc cggtacgctg gtggcgggtt tggcctatgc cttcctgaaa
    10861 cgtccggaag tggacgcagt agcgaaagca gcgtaataaa aggtgtgtta cagggcagaa
    10921 atttcctgcc ctgtaacatt tgatgagcgc aaagggaatg accagtttgt gaatgtaatt
    10981 ttatgaattt aaatggtttt atctttcagt agaaacctga aatgttcatc aaacactaag
    11041 gttatttcct caagggcaga ttattttctg ccctttttta gtgttcctgt tctgtgcatc
    11101 ccgtcacaaa ttcctcactt ttgtaattca ggcatttaat cgcattttgt gagcctgctc
    11161 aaatctgccc attttgcctc tctggcggca atttacttta cgtaaaacat gcatactatg
    11221 agcacatgtt taaatatgaa cacatgttta aaccgtcttt gcagtatcta tattcttgtg
    11281 gattgacagg attggtaatt ttttaccaga ggacgtgcta tgcgcgaaaa ggattatgtc
    11341 gtaattatag gttcggcgaa tattgatgtc gccggatatt cacatgaatc attaaattat
    11401 gcggattcaa atccaggtaa aataaagttt acgcctggtg gagtagggcg caatattgca
    11461 caaaacctgg cgttgctggg gaataaagcc tggctactga gcgccgtagg cagtgatttt
    11521 tatggtcaat cgctgctaac gcaaaccaat caatctggtg tttatgtcga taaatgcctg
    11581 attgtgccgg gagaaaatac gtcgagctat ttatcattac tcgataatac cggtgaaatg
    11641 ctggttgcta taaatgacat gaatattagc aacgctatta cagctgaata tctcgcacag
    11701 cacggtgaat ttattcagag ggcaaaggtc attgtcgcgg actgtaatat cagtgaagag
    11761 gcactggcat ggattctgga taatgccgcc aacgtacccg tatttgtcga tcctgtttcc
    11821 gcgtggaaat gtgtcaaagt gcgcgaccgt ctaaatcaaa tccacactct caagccaaac
    11881 cgccttgaag cggaaaccct gagtgggatt gctttgtcag ggcgtgaaga tgtggcaaaa
    11941 gttgctgcct ggttccatca acatggcctg aaccgactgg tattgagcat gggcggcgac
    12001 ggcgtttatt acagtgatat cagcggtgaa agtggttggt ctgcgccgat caaaaccaat
    12061 gtcattaatg ttaccggagc gggcgatgcc atgatggcgg gacttgcttc gtgttgggta
    12121 gacggaatgc cgtttgccga atctgttcgt ttcgcacagg gatgttcgtc aatggcgctc
    12181 tcctgtgaat acaccaataa ccccgattta tcgattgcca acgttatatc gttagtggag
    12241 aacgcagaat gtctgaatta aaaatttccc ctgaattatt acaaatttcc ccggaagtgc
    12301 aggacgcttt aaaaaacaaa aaaccggttg tggcgctgga atcgaccatt atttctcacg
    12361 ggatgccgtt cccacaaaat gcccagaccg caattgaagt tgaagaaact attcgtaaac
    12421 agggcgctgt acctgccact atcgccatta ttggcggcgt gatgaaagtg ggtttaagca
    12481 aagaagaaat tgaattgctg ggtcgtgaag ggcataacgt gaccaaagtt agtcgtcgcg
    12541 atttaccttt tgttgttgcc gccggaaaaa atggtgcaac caccgttgct tcaacgatga
    12601 ttattgcggc gcttgccgga attaaagtat ttgccaccgg gggaattggt ggtgtgcatc
    12661 gcggggcgga acataccttc gatatttctg ccgatttgca agaactggcg cacaccaatg
    12721 tcaccgtcgt ttgtgccggg gcgaaatcta ttctcgattt aggcttaact accgaatatt
    12781 tagaaacttt cggtgtaccg ctaattggct atcagaccaa agcgctgcct gcgtttttct
    12841 gccgcaccag cccgtttgac gtgagcattc gtctcgacag cgccagcgaa attgcccgtg
    12901 caatggcggt gaaatggcaa agcgggctga acggtggcct cgtggtagcg aacccgatcc
    12961 cggaacagtt tgctatgccg gaacacacta tcaatgcggc gatcgatcag gcggtagctg
    13021 aagctgaagc gcagggtgtt attggtaaag aaagtacgcc attcctgctg gcgcgcgttg
    13081 ctgagttgac cggcggtgac agcctgaaat ccaacatcca gctggtgttc aacaacgcca
    13141 tcctggcgag tgaaatagcc aaagaatacc agcgtctcgc gggttaattt ctgacgggca
    13201 gaaagccgtc gcccgtactc atcaagcata ccaaaacctg gcgctgacgc cgggtttact
    13261 ggcatgaagg gaaaatcatt atggatataa tgagaagtgt tgtggggatg gtggtgttac
    13321 tggcaatagc atttctgttg tcagtgaata aaaagagcat cagtttgcgc acggttggag
    13381 ccgcactgct gctgcaaatc gctattggtg gcatcatgct ctacttccca ccgggaaaat
    13441 gggcagtaga acaggcggca ttaggcgttc ataaagtgat gtcttacagt gatgccggta
    13501 gcgccttcat ttttggttcg ctggtcgggc cgaaaatgga tgtcctcttt gacggtgcgg
    13561 gttttatctt cgcctttcgc gtacttccgg cgattatctt cgtcactgcg ctcatcagtc
    13621 tgctgtacta cattggcgtg atggggctgc tgattcgcat ccttggcagc attttccaga
    13681 aagccctcaa catcagcaaa atcgaatctt ttgttgcggt taccactatt ttcctcgggc
    13741 aaaatgagat cccggcgatc gttaaaccgt ttatcgatcg catgaatcgc aacgagttgt
    13801 ttaccgcaat ttgtagcggg atggcgtcca ttgctggttc gatgatgatt ggttatgccg
    13861 gaatgggcgt accaattgac tacctgttag cggcatcgct gatggcgatc cctggtggta
    13921 ttttgtttgc acgtattctt agcccagcca ccgagccttc gcaggtgaca tttgaaaatc
    13981 tgtcgttcag cgaaacgccg ccaaaaagct ttatcgaagc ggcggcgagc ggtgcgatga
    14041 ccgggctgaa aatcgccgct ggtgtggcga cggttgtaat ggcgtttgtc gcaattattg
    14101 cgctgatcaa cggcattatc ggcggaattg gcggctggtt tggtttcgcc aatgcctctc
    14161 tggaaagtat ttttggctat gtgctggcac cgctggcgtg gatcatgggt gtggactgga
    14221 gtgatgccaa tcttgcgggt agcctgattg ggcagaaact ggcgattaac gaattcgtcg
    14281 cttacctgag tttctcccca tacctgcaaa cgggcggcac gctggaagtg aaaaccattg
    14341 cgattatctc ctttgcgctt tgtggttttg ctaactttgg ttctatcggt gttgtcgttg
    14401 gggcattttc ggctatttcg ccaaaacgcg caccggaaat cgcccagctt ggtttacggg
    14461 cgctggcagc agcaacgctt tccaacctga tgagcgcgac cattgccggg ttctttattg
    14521 gtttagcttg agtgtcattg ccggatgtac gcgcttatta agcgtcacat ccggcaatcg
    14581 ctctattgca aataattttt actgcatcat ccctgagaat ttattttccg ggtccatctc
    14641 cagtgccagt cctgacaact gctttctgtt tttaatgaga taccctttct tgcttttaat
    14701 taataaaccg tcgtgaatga attgcgcgag aacatataaa agatgtcgat aagaaacacc
    14761 taaatactct gccgcttgcg tatgcttttc gtgataaaga tcaccttcct gcgagagtaa
    14821 tataaaagct gccaggcgat tcactaacgg aaatgattga ttctgagtta aagaaacaat
    14881 attacgataa tttttatgac ttaaggtgac gcagagtttt cgtaaaaata gcgtgtcgtt
    14941 taataacagc gggcggtaat gtttcatagg gagcgcaagg caccaacact cttcaatagc
    15001 ctgcaccgca cgcggttcat ggtctttgtc gattaactca atctcgccaa taaaacaggg
    15061 agcggcaaag aaatcgatca gcgacacgcg accattagca agcgtggcgt aaagcctggc
    15121 acggcctcgc gtcagataaa acagccatga cggctgttgc ccttcctgca caatgtagtc
    15181 acgcgctaaa aaatgaaaca gccgcgtatc ggctgaaaca tccgttgaaa agcaatcctt
    15241 aaacgcagat tcactcatca attgctgctt aaggtcatta ttgtggattt ctttcacgat
    15301 gtttatccgc tatgagattt ctcatattta gcatacttac gctgttgtta agattcaatc
    15361 atcggcccgg tcgaattttc tttgctgtag aaaaaataac gcaactggaa acagaggaaa
    15421 taaacaatgg aaaagagaaa aattattctg gattgtgatc cgggtcatga tgatgctatt
    15481 gccataatga tggcagcgaa acatccggcg attgatttat taggtatcac cattgtggcg
    15541 ggtaatcaga cgcttgataa aacattaatt aatggcctga atgtttgcca gaaactggag
    15601 attaatgttc cggtttatgc ggggatgcca cagcccatta tgcgtcaaca aatcgttgcc
    15661 gataatattc acggtgaaac cggactggat ggcccggtat tcgagccgct gacccgccag
    15721 gcagaaagca ctcatgcggt gaaatatatc attgataccc tgatggcaag cgatggcgat
    15781 atcactttgg tgccggttgg cccactttca aatatcgcgg tggcaatgcg tatgcaaccc
    15841 gcgatcctgc cgaaaatccg tgaaattgtg ctgatgggcg gcgcttacgg tactggcaac
    15901 ttcaccccat ctgccgagtt caacatcttt gctgacccgg aagccgcacg cgtggtgttc
    15961 acctccggcg ttccattagt gatgatgggc ctcgatctca ccaaccagac cgtttgcacc
    16021 ccggacgtga ttgctcggat ggaaagggca ggcggccctg ccggagagct gttcagcgac
    16081 atcatgaact tcactctcaa aacccagttc gaaaactacg gcctggctgg cggcccggtg
    16141 catgacgcca cctgcatcgg ttatctgatt aaccctgatg gcattaaaac ccaggagatg
    16201 tacgtcgaag tggacgttaa cagcggccct tgctacgggc gtacggtctg cgacgagctg
    16261 ggcgttcttg gcaagcccgc caataccaaa gtcggcatca ctattgatac tgactggttc
    16321 tggggattag tcgaagagtg cgtgcgcggc tacatcaaaa cccattaagt gttgataaca
    16381 agccgggaca gtatcgcaag atgcaggccc ggctatttct gatgggcaga aagccgtcgc
    16441 ccaaaatgac atgaagagat aataactatg gatgtcatga gaagtgttct gggaatggtg
    16501 gtattgctgg caattgcgtt tttgctgtca gtaaacaaga agaagatcag cctgcgtacc
    16561 gttggcgcgg cgttagtgtt acaggtcgtg attggcggca ttatgctttg gttaccacca
    16621 gggcgttggg tcgcagaaaa agtcgctttt ggcgtgcata aagtgatggc gtacagcgat
    16681 gcaggtagcg catttatctt tggttctctg gtcggaccaa aaatggatac cttatttgat
    16741 ggcgcaggat ttatctttgg tttcagggta ttaccggcaa ttatcttcgt tacagcactg
    16801 gtgagtattc tctactacat cggtgtgatg gggatcttaa ttcgcattct cggcggtatc
    16861 ttccagaaag cattgaatat cagcaagatc gagtcattcg tcgcggtcac taccattttc
    16921 ctcgggcaaa acgaaattcc ggcaatcgtc aaatccttta tcaatcgtct gaatcgcaat
    16981 gaattattta cagcgatttg tagcgggatg gcctcgattg ctggttcgac aatgattggt
    17041 tacgccgcac tgggcgtgcc tgtggaatat ctgctggcgg catcgttaat ggcgatcccc
    17101 ggcgggatct tgtttgcccg cctgttaagc ccggcaacag aatcttcgca ggtttccttt
    17161 aataacctct ctttcaccga aacaccgcca aaaagcatta ttgaagccgc tgcgacaggg
    17221 gcaatgaccg ggctgaaaat cgccgcaggt gtggcgacag tagtgatggc atttgtcgcc
    17281 atcattgcgt taattaacgg tattatcggc ggcgttggcg gctggtttgg ctttgaacat
    17341 gcctcgctgg agtccatttt aggttacctg ctggccccac tggcgtgggt aatgggtgtt
    17401 gactggagtg atgcgaatct tgccgggagt ttgattggac agaaactggc aataaatgaa
    17461 tttgtcgctt acctcaattt ctcaccttat ctgcaaacag ctggcactct ggatgctaaa
    17521 accgtggcga ttatttcctt cgcgttgtgc ggtttcgcta actttggttc tatcggggtg
    17581 gtggtggggg cgttttctgc ggttgcgcca caccgtgcac cggaaatcgc ccagcttggt
    17641 ttacgcgcgc tggcggcggc gacactttct aacctgatga gtgcaaccat tgcaggattc
    17701 tttattggct tagcgtagtg cgcgagttat gagccggata agatgtagtc aagcgtcgaa
    17761 tccggcaaca ttgttacacc atcggcacta atgaaagcgc gttatcggca gacagggtag
    17821 ggttgtttaa gctgccgctg gcgcgtgaaa ttgccgcgca ggccatcgca aaacgggcgc
    17881 tgtcacggaa attgtttccc tccagaaagc tatataccag gcccgccata aaaccatcgt
    17941 cagcaccaaa actgtctact gtcgtatgcg ctggcgctgt cagcaaaaat tgctctccat
    18001 ccttttcgct gcaataaact gactcatcgg gcaaataaac aaacagttgc tgaacgcctt
    18061 gctgatgcaa cgcattcact gcggcattac ggtcagcatc gctggtgatc gcctgtcccc
    18121 ataaaatttc cagctccggt aaagtgggtt tcagggtgtg aatatgcgcc agccagtgtt
    18181 tgattttgcc cgctttgaat tctgaaacgg tatcgacaaa caccgggatt tcatcagcga
    18241 gggtaaagac ccattccagc gcctcggctg tcaggttaca atcagcaagt accacgcccg
    18301 catgacgaag taaatcgcgc gacccgttca gtagttgcgg tgtcaactga tccagcagat
    18361 gggtatcgtt aatcgccagc acggtttgat cgtctcgatt ggcaattgcc agatacgtcg
    18421 atgtgctttg gccatgtaaa cgaacgcagc cggagacatt cacgccggcg cggcgcgttt
    18481 cttccaggag catttcgcca taaaagtcat caccaatcac tgaaagcaaa tggacgtcgc
    18541 ggcctaacag cgccaggttg tgggcgatat tgcgtccaac gccgcctgcc gagcaatgaa
    18601 tggtaccggg atgagaagcc gcttgcgggt aacggatatc cgccatcccg cgaatatcca
    18661 tattgattgt ccccaccact acgcagtatt cctgctcggt gagaatgtaa cctttgcctt
    18721 taatccggcc tttgcgcatt aaatccataa tatgcgccgc aacgcgcgaa cggctgattt
    18781 gcagcatatc cgcaatttcg ttctgctgaa tcagcgggtt acgccgtaaa attgcaagga
    18841 tctccttctc ccggttgttc atcttcaggc taccgctttt tcagtttgtt gcgctttcag
    18901 ccaggcgatc tcttctgccc agatatccgg gttgatggtt tcaaggatca gcggaatgcc
    18961 gtcgaaacgg tcgtcctgca tgatccagcg gaacgcatca tgaccaatat tgccttcacc
    19021 gaggctatga tggcggtcaa cgcggctgcc aaaggtgctt ttcgcatcgt taaggtgcat
    19081 cccgcgcaga tacttaaagc caacaatacg ggcaaaatcc gcgaacgttt tctcgcattc
    19141 ggcagaagta cgcaaatcat acccggcagc aaaagcatgg caggtatcaa tgcagacgcc
    19201 gacgcgggat ttatcttcca cgccgtcgat aatcgccgcg agatgttcga atttaaaccc
    19261 taagttactg ccctgaccgg cggtgttttc aatcaccgct gtcacacctt gagttttatc
    19321 cagcgcaatg ttgatggatt cggcaatacg cgcaaggcaa tcctcttctg aaatctgcat
    19381 cagatggctg ccagggtgga agttgagcaa agaaagcccc agctgttcgc aacgctgcat
    19441 ttcatctata aacgcatcgc gcgatttttc cagagcttca gcgaccggat gtccgaggtt
    19501 aatcagataa ctgtcgtggg gaagaatttg cgccgatgtg tagtgatatt tttcacaggc
    19561 ggctttgaat tcatcgatgg tttgcgtcgt gagcggtgcg gcacgccact gacgttggtt
    19621 tttggtgaac aaggcaaacg cggttgcgtc gatttcggcg gcgcgaattg cggcatttgc
    19681 cagaccgcca gcagcactaa cgtgcgctcc aatgtatttc atgcgaggac tcctgttaaa
    19741 cccgctggag gaaaacggta atgatagcgg gttaacagga gcaagatgta gtggtttatg
    19801 cgatgacgct ttgaatcaca tagttaatcg caccaccacc aacaatcagc caggcaaaca
    19861 gtaccagtgc catcagcaga ggtttcgccc cggctttttt cagcgcgctg acgtgagtgg
    19921 tcagacccag cgccgccatc gccattgcca gcaggaaggt atccagcgtt accagcatgt
    19981 tcaccacgct ctgcggtaac aggtggaacg agttaaagat ggcaactacg atgaacaaga
    20041 tggcaaacca cggaatagtg attttgcttt tctcgccgct gttcgcccca gacagctgtt
    20101 taacacgcgc cgccagcagg atgaggaacg gagccagcat catcacgcgc agcattttgg
    20161 aaataactgc tgcgttttct gcatccgggc tgatggcatg acctgccgcc accacctgcg
    20221 ccacttcgtg cacagtagaa ccaatgtaga taccgaaagt ttccggacta aaccattgag
    20281 acatcagcgg atatatcgcc gggtagagga aaatcgcgac ggtcccgaag ataacaacgg
    20341 ttgcaacagc cacggttact ttactggctt ccgctttcac taccggctca gtcgccagta
    20401 ccgcggcagc accacagata ctgctaccgg caccgatcaa ccagctggtg tgcttatcca
    20461 gaccaaacac tttctgcccc aggaagcaag ccagcaggaa ggtactggac agcgtcagca
    20521 cgtcaatgat gatcccactg ataccgacat cggcaatttg cgagaacgtc agacggaagc
    20581 cataaagaat gatacccaga cgtaataaat attgcttggc aaacagcaca ccaccgtcac
    20641 agcttttcca gatgtgcgga tagatggtgt tgcctaaaac catccccaac aagattgcga
    20701 gggtgagggc actaaacccg gcacccgcaa ccgcgggaat ggaaccaccc cacagggcga
    20761 ccccggtgat aactgcactc agggctaacc ccggaataaa atgccacagt gtacgatgtt
    20821 gtttctgtaa ggtgatattc gtcataaccc tctcctttaa ccgggcataa ggttacgact
    20881 aactggttta aaaataaaat tgattatata tttataatta atctttataa gtggtaagcg
    20941 actatgcaca tcaccctccg gcagttggaa gtttttgcag aagtattgaa aagtggatca
    21001 accacccagg cgtcggtgat gctggcgttg tcgcaatcag cagtgagcgc agccttgacc
    21061 gatctggaag ggcagcttgg cgtgcaactg tttgatcgcg tggggaaaag actggttgtt
    21121 aatgaacacg ggcggctgct ctatccgcgc gcgttggcac tgcttgaaca ggcggttgaa
    21181 atcgaacaac tgtttcgcga agacaacggc gcgattcgta tctatgccag tagtactatc
    21241 ggtaactaca ttctgcctgc agtcattgcc cgttatcgcc atgattatcc gcagttgccg
    21301 attgaactta gcgttgggaa cagccaggac gtgatgcaag cggtgctgga tttccgcgtt
    21361 gatattggct ttattgaagg accgtgccac agcactgaaa tcatttctga accgtggctg
    21421 gaagacgagc tggtggtttt cgccgcgccg acttcgccat tggctcgtgg tccggtcacc
    21481 ttagaacagc tggccgctgc gccgtggatc ctgcgtgaac gcggctccgg cacgcgggag
    21541 attgtcgatt atctgttgct gtcacattta ccgaagtttg agatggcgat ggaattaggt
    21601 aactccgagg caatcaaaca tgcggtgcgt catgggttgg gaattagttg cctgtcgcga
    21661 cgggtgattg aagatcaatt gcaggcaggc acattaagtg aagttgcggt ccctctgccg
    21721 cgcctgatgc gtacgttgtg gcgtatacat catcggcaaa aacacctttc caacgcgcta
    21781 cggcgctttc tggactattg cgatcccgcg aatgtaccgc gttaagttgc tgtacaagaa
    21841 catgctggtg ctgtgtcgat ttcgtgacgc agcgccctca gcatgcattc gccagaaaag
    21901 agattggctg ctttacttat aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat
    21961 ttctgccgga aggattgcca atcgtctgct acaatcgcgc ctcattttta agatggatag
    22021 catttttgta tggtttccga aactaaaacc acagaagcgc cgggcttacg ccgtgaatta
    22081 aaggcgcgtc acctgacgat gattgccatt ggcggttcca tcggtacagg tctttttgtt
    22141 gcctctggcg caacgatttc tcaggcaggt ccgggcgggg cattgctctc gtatatgttg
    22201 attggcctga tggtttactt cctgatgacc agtctcggtg aactggctgc atatatgccg
    22261 gtttccggtt cgtttgccac ttacggtcag aactatgttg aagaaggctt tggcttcgcg
    22321 ctgggctgga actactggta caactgggcg gtgactatcg ccgttgacct ggttgcagct
    22381 cagctggtca tgagctggtg gttcccggat acaccgggct ggatctggag tgcgctgttc
    22441 ctcggcgtta tcttcctgct gaactacatc tcagttcgtg gctttggtga agcggaatac
    22501 tggttctcac tgatcaaagt cacgacagtt attgtcttta tcatcgttgg cgtgctgatg
    22561 attatcggta tcttcaaagg cgcgcagcct gcgggctgga gcaactggac aatcggcgaa
    22621 gcgccgtttg ctggtggttt tgcggcgatg atcggcgtag ctatgattgt cggcttctct
    22681 ttccagggaa ccgagctgat cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac
    22741 attccacgcg cggtacgtca ggtgttctgg cgaatcctgt tgttctatgt gttcgcgatc
    22801 ctgattatca gcctgattat tccgtacacc gatccgagcc tgctgcgtaa cgatgttaaa
    22861 gacatcagcg tcagtccgtt caccctggtg ttccagcacg cgggtctgct ctctgctgca
    22921 gcggtgatga acgcagttat tctgacggcg gtgctgtcag cgggtaactc cggtatgtat
    22981 gcgtctactc gtatgctgta caccctggcg tgtgacggta aagcgccgcg cattttcgct
    23041 aaactgtcgc gtggtggcgt gccgcgtaat gcgctgtatg cgacgacggt gattgccggt
    23101 ctgtgcttcc tgacctccat gtttggcaac cagacggtat acctgtggct gctgaacacc
    23161 tccgggatga ccggttttat cgcctggctg gggattgccg ttagccacta tcgcttccgt
    23221 cgcggttacg tattgcaggg acacgacatt aacgatctgc cgtaccgttc aggtttcttc
    23281 ccactggggc cgatcttcgc attcattctg tgtctgatta tcactttggg ccagaactat
    23341 gaagcgttcc tgaaagatac cattgactgg ggcggcgtag cggcaacgta tattggtatc
    23401 ccgctgttcc tgattatttg gttcggctac aagctgatta aaggaactca ctttgtacgc
    23461 tacagcgaaa tgaagttccc gcagaacgat aagaaataag ttttctccct tccttgctaa
    23521 gccctctcaa ccgagagggc tttttcaatt ccatttccct gacaaatcat gcggatataa
    23581 aatttaacat ttggattgat aattgttatc gtttgcatta tcgttacgcc gcaatcaaaa
    23641 aaggctgaca aatcagaggc tggtctggct ttctgggatg atcacctgca tacaaataag
    23701 tccaccgcga tgctgccgta cgcaagggga cgtgaagaag atgtgagcga taacccattt
    23761 tattttcgta gttacctcat ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt
    23821 cgggctgtgt ttgtccgcta tttcttgtgc atggcctgtg ttagcggtcg atgatgatgg
    23881 cgaaacgatg gttgtcactg catcttccgt tgagcaaaac cttaaagatg cacctgccag
    23941 tatcagcgtc attacctagg aagacctgca gcgaaaaccg gtacagaatc tgaaggatgt
    24001 cctcaaagaa gtgcctggcg tacaactgac gaacgaaggg gataaccgta agggcgttag
    24061 tattcgtggt ctggacagca gctacaccct gattctcgtc gacggtaaac gcgttaactc
    24121 ccgcaatgcc gtcttccgcc acaatgattt cgatctgaac tggatcccgg tcgattccat
    24181 cgaacgtatt gaagtggtcc gtggcccgat gtcgtcgctg tacggttccg atgcgctcgg
    24241 cggtgtagtg aatatcatca ccaaaaaaat cggtcagaaa tggtcgggca ccgttaccgt
    24301 cgataccacc gttcaggaac atcgcgatcg cggtgatacc tataacggtc aattctttac
    24361 cagcggacca ttaattgacg gcgtgctggg aatgaaagct tacggcagcc tggcaaaacg
    24421 tgaaaaggat gacccgcaaa actcaacgac caccgatacc ggagaaacgc cgcgtattga
    24481 aggattctcc agccgcgacg gcaatgtcga atttgcctgg acaccgaatc aaaatcacga
    24541 ttttactgcc ggatacggtt tcgaccgtca ggatcgtgat tccgactcgc tggacaaaaa
    24601 ccgcctggaa cgccagaact actccgtcag ccataatggg cgttgggatt acggcaccag
    24661 cgaactgaaa tactacggtg agaaagtcga gaacaaaaac cctggcaaca gcagcccgat
    24721 aacttccgaa agcaatacgg tcgacggcaa atacacgttg ccgctgacgg cgattaatca
    24781 gtttctcacg gttggcggtg aatggcgtca cgacaaactt agcgatgcgg tgaacctgac
    24841 tgggggaacc agctccaaaa catctgccag ccagtacgcg ctatttgttg aagatgaatg
    24901 gcggatcttc gagccgctgg cgctgacgac cggcgtacgt atggacgatc atgaaaccta
    24961 cggtgaacac tggagtccgc gtgcctacct ggtttataac gccaccgaca ccgtaacggt
    25021 gaaagggggc tgggcgacgg catttaaagc accttctctg ttgcaactta gccctgactg
    25081 gacgagcaat tcctgccgtg gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga
    25141 aaccagcgaa agttgggagc tggggcttta ctacatgggc gaagaaggct ggctggaagg
    25201 ggttgaatcc agcgttaccg ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg
    25261 tacgtctgac gtcaatgctg caccaggcta ccaaaacttt gttggtttta cagcgaacgg
    25321 tgtgccggta tttagctact acaacgttaa caaagcacgt attcagggcg tggaaaccga
    25381 gctgaaaatt ccgttcaacg atgaatggaa actgtcactc aactacacct acaacgatgg
    25441 tcgtgatgtc agcaacggcg aaaataaacc tttatccgat ctgccgttcc ataccgctaa
    25501 cggtacgctg gactggaaac cgctggcgct ggaagactgg tcattctatg tttccggaca
    25561 ctataccggg cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat
    25621 ctggaatacc ggtgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct
    25681 gaaccttggc gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg
    25741 ttactttatg gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac
    25801 tttcacttca gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg
    25861 aagtagtaac tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg
    25921 cgggcggctt ctgccagcac tgcgggttgc agctggtcgg caagaaactg atcattatca
    25981 ccctgatcga taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcacaa
    26041 ctgtcccatt ccagccatgc atttttgtcc tcacctaaat agctgctaaa cgctttaatg
    26101 ccccagggta cgctgcatgg attcacaatc ggcgcaaagg cggaaacgct ggtgtatttg
    26161 cccgggtttt tcagcgccat aatcagcgca ccgtgaccac ccattgaatg accgctaata
    26221 gcgcagcggt cgctgacatt aaattgcgac tgaaccagcg ccggtaattc atcgcgcaga
    26281 taatcataca tccggtaatg cgtcgcccac ggcggttgcg tggcattaag ataaaagcct
    26341 gcgccctggc ctaaatcgta gccatcgtcg ttggcaacct gttcgccgcg cgggctggtg
    26401 tctggcatca ccagtacaat ccccagttct gccgccacgc gctgggcacc cgccttggtg
    26461 gtgaagttct cgtcattgca ggttaatccg gaaagccagt acagcactgg tggcggggtg
    26521 tgatcacgag gtggtgggag aaagatactg aacgtcatcg gacagtttaa ggtactggag
    26581 tcgtgtcgcc agcgttgctg ccagccttca aaacagcggt gctcttcgag catttccatg
    26641 cgtggctcct tgttgtgttg tttgcaattt cttcatcata ccgtttattc aatgtgctgt
    26701 gagtaacttt cacttccgta tttgcataac gatgttttaa catctgctga tgaaaggcag
    26761 cggcaattac aataattatc gctgtgaata ctggattatg tgcgccgcct cacgcacaat
    26821 aatcaggctg taaatcagct taataacttt gcccccacgc agggcggagg cgtcacacct
    26881 gcaggagaaa tcataaatgc catcactcag taaagaagcg gccctggttc atgaagcgtt
    26941 agttgcgcga ggactggaaa caccgctgcg cccgcccgtg catgaaatgg ataacgaaac
    27001 gcgcaaaagc cttattgctg gtcatatgac cgaaatcatg cagctgctga atctcgacct
    27061 ggctgatgac agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg tcgatgaaat
    27121 tttctccggt ctggattacg ccaatttccc gaaaatcacc ctcattgaaa acaaaatgaa
    27181 ggtcgatgaa atggtcaccg tgcgcgatat caccctgacc agcacctgtg aacaccattt
    27241 tgttaccatc gatggcaaag cgacggtggc ttatatcccg aaagattcgg tgatcggtct
    27301 gtcaaaaatt aaccgcatcg tgcagttctt tgcccagcgc ccacaggtac aggaacgtct
    27361 gacccagcaa attctcattg cgctacaaac gctgctgggc accaataacg tggctgtctc
    27421 tatcgacgcg gtgcattact gcgtgaaagc gcgtggcatc cgcgacgcaa ccagtgccac
    27481 aacaacgacc tctcttggtg gattgttcaa atccagtcag aatacgcgcc acgaatttct
    27541 gcgcgctgtg cgtcatcaca actgattaaa aggcaggaac catggggcgc aacgtcacgc
    27601 tcgattttgt tcgcggcgtc gccattctgg ggatcctgct attaaacatc agcgcctttg
    27661 ggctaccaaa ggcggcttat cttaatcccg cctggtacgg cgctattacg ccgcaggatg
    27721 catggacctg ggcatttctc gatctcatcg gccaggtgaa attcctcacg ctttttgcgc
    27781 tgctgtttgg tgctggcctg caaatgttgc tgccccgtgg cagacgctgg atccagtcgc
    27841 ggttaacgct gttagtcttg ctgggcttta ttcacggttt attgttctgg gacggcgata
    27901 ttctgctggc ttacgggctg gtgggcttaa tctgctggcg gctggtgcgc gatgcgccat
    27961 cggtaaaaag cctgtttaat accggcgtca tgctttatct ggtggggctt ggcgttttgc
    28021 tgttattggg gttgatttcc gatagccaga ccagccgcgc ctggacacct gatgcatcgg
    28081 ctattttgta tgaaaagtac tggaaacttc acggtggtgt tgaagcgatc agtaatcgtg
    28141 ccgatggtgt tggcaacagt ttactggcac tgggcgcaca gtatggctgg caactggcag
    28201 ggatgatgct cattggtgcg gcgctgatgc gcagcggctg gctgaaaggg cagttcagct
    28261 tacgtcacta tcgtcgtact ggttttgtgc tggtggcgat tggggtgacc attaaccttc
    28321 ctgccatcgc cctgcaatgg cagctggact gggcgtatcg ctggtgtgcc tttttacttc
    28381 agatgccgcg ggaactgagt gcgccgtttc aggcgattgg ctatgcgtcg ctgttttatg
    28441 gcttctggcc gcaattgagc cgctttaagc tggtgcttgc gatcgcctgc gtcggacgga
    28501 tggcgctgac caactatcta ttgcaaacgc tgatttgtac cacgcttttt taccacctcg
    28561 gtttgtttat gcattttgac cgcctggagc tgctggcgtt tgttattccg gtatggctgg
    28621 cgaatatcct cttctctgtt atctggctgc gttacttccg ccaggggccg gtggaatggc
    28681 tctggcgtca gttaactttg cgtgcagctg ggccggcaag atctaaaaca tcaagataac
    28741 gatctggatc acattcgtta acaaaacggc tgtaaccgtt tccattgctg tgactcgatt
    28801 cacgaagtcc tgtattcagt gctgacaaaa tagccgccag caagcagtca tttactgcaa
    28861 tctcataaca ggtagtgaat atgatcacca ttcgtgatgt agcgcgtcag gctggcgtct
    28921 ctgtggcaac ggtttcccgg gtgctcaata acagcacgct ggtcagtgcc gacacgcgtg
    28981 aagcagtaat gaaagccgtg agtgagctgg attatcggcc aaacgccaat gcccaggcgc
    29041 tggcaactca ggttagcgat accattggcg tggtggtgat ggacgtttct gatgcgtttt
    29101 ttggtgcgct ggtaaaagcg gtggatttgg tcgcccagca gcatcagaaa tacgtgctaa
    29161 taggcaatag ctatcatgaa gcggaaaaag agcgtcacgc cattgaggtg ttaattcgcc
    29221 agcggtgtaa tgcgttgatt gttcactcaa aagcattgag tgatgatgaa ctggcacaat
    29281 ttatggataa cattcctggt atggtgttaa tcaaccgcgt tgtgccgggg tacgcccatc
    29341 gttgcgtttg cctggataat ctcagcggtg cccgaatggc gacgcgcatg ttgctgaata
    29401 acggtcatca acgtattggt tatctttctt ccagtcacgg cattgaagat gacgccatgc
    29461 gtaaagcagg ctggatgagt gcgttgaaag agcaggatat tattccgccg gaaagctgga
    29521 ttggcacagg tacgccggac atgccgggcg gtgaggcggc gatggttgaa ctgctggggc
    29581 gcaatctaca acttaccgct gtatttgctt ataacgacaa tatggccgct ggcgcactga
    29641 cagcattaaa agataatggc attgcgattc cgttacatct ctccatcatc ggtttcgatg
    29701 atattcccat cgcccgttac accgacccgc aattaacgac tgtgcgttat cccattgctt
    29761 caatggctaa attagccacc gaactggcct tgcagggggc agcaggcaat attgatccgc
    29821 gtgccagcca ctgttttatg ccgacgttag tgcgtcgcca ttctgtcgca acgcgccaga
    29881 atgcggcggc gatcactaac tcaacaaatc tggcgatgta accgctttca atctgtgagt
    29941 gatttcacag tatcttaaca atgtgatagc tatgattgca ccgttttaac gttgtaaccc
    30001 gtatgtaaca gtgaataatc actttcaccg aggtaatagc gtcataacaa caattaaagc
    30061 cgttttctgg agcgttaccg ggcatggaag aacgaatttt aaaaagtgag cttcggcgtt
    30121 cagtaacact tcattaactc tactgccccg ccgagcattt atctcaagca ctaccctgca
    30181 taagaaaaac cggagatacc atgaataaga aggtgttaac cctgtttgct gtgatggcca
    30241 gcatgttatt cggtgccgct gcacacgctg ctgatactcg cattggtgta acaatctata
    30301 agtacgacga taactttatg tctgtggtgc gcaaggctat tgagcaagat gcgaaagccg
    30361 cgccagatgt tcagctgctg atgaatgatt ctcagaatga ccagtccaag cagaacgatc
    30421 agatcgacgt attgctggcg aaaggggtga aggcactggc aatcaacctg gttgacccgg
    30481 cagctgcggg tacggtgatt gagaaagcgc gtgggcaaaa cgtgccggtg gttttcttca
    30541 acaaagaacc gtctcgtaag gcgctggata gctacgacaa agcctactac gttggcactg
    30601 actccaaaga gtccggcatt attcagggcg atttgattgc taaacactgg gcggcgaatc
    30661 agggttggga tctgaacaaa gacggtcaga ttcagttcgt actgctgaaa ggtgagccgg
    30721 gccatccgga tgcagaagca cgtaccactt acgtgattaa agaattgaac gacaaaggca
    30781 tcaaaactga acagttacag ttagataccg ctatgtggga taccgctcag gcgaaagata
    30841 agatggacgc ctggctgtct ggcccgaacg ccaacaaaat cgaagtggtt atcgccaaca
    30901 acgatgcgat ggcaatgggc gcggtagaag cactgaaagc acacaacaag tccagcattc
    30961 cggtgtttgg cgtcgatgcg ctgccagaag cgctggcgct ggtgaaatcc ggtgcactgg
    31021 cgggcaccgt actgaacgat gctaacaacc aggcgaaagc gacctttgat ctggcgaaaa
    31081 acctggccga tggcaaaggt gcggctgatg gcaccaactg gaaaatcgac aacaaagtgg
    31141 tccgcgtacc ttatgttggc gtagataaag acaacctggc tgaattcagc aagaaataag
    31201 actgattggg cgcagtcgtt tactgcgccc ttattgtatg gccaacaagg taaaattata
    31261 tggtcagctc aacgactccg tcttccgggg aatacttgtt ggaaatgagc ggtatcaaca
    31321 agtcttttcc aggtgttaag gcacttgata acgttaattt aaaagtccgg ccacattcta
    31381 tccatgcatt aatgggggaa aacggcgcag gaaaatcaac attattaaaa tgcctgtttg
    31441 gtatttatca aaaagactcc ggcaccattt tattccaggg taaagagatc gatttccatt
    31501 ctgccaaaga agcgctggaa aatggtattt cgatggtaca ccaggagtta aacctggtat
    31561 tacaacgttc ggtgatggac aacatgtggc tggggcgata tcccaccaaa ggcatgtttg
    31621 tcgatcagga caaaatgtac cgcgaaacca aagcgatttt tgatgaactg gatattgata
    31681 tcgatccgcg tgcgcgcgtc ggcacattat ccgtttcgca aatgcagatg atcgaaatcg
    31741 ccaaagcgtt ttcctataac gcgaaaattg tgattatgga tgaaccgact tcttcgttaa
    31801 ccgaaaaaga ggtcaatcat ctgttcacta ttattcgtaa attaaaagag cgcggctgcg
    31861 gtattgttta tatctcacat aaaatggaag aaatcttcca gttatgtgat gaagttaccg
    31921 tattgcgcga cggtcagtgg atcgccactg aaccgctggc aggactgacg atggacaaga
    31981 tcatcgccat gatggttggg cgttctctta accagcgttt ccctgacaaa gaaaacaagc
    32041 cgggcgaagt gattcttgaa gtgcgtaacc tgacgtcgct gcgccagccg tcgattcgcg
    32101 atgtctcgtt tgatctgcat aaaggggaga ttctcggtat agccggtctg gtgggagcga
    32161 aacgtaccga tattgttgag acgttatttg gtattcgcga gaaatcggct ggcaccatta
    32221 cgttgcacgg caaaaagatc aataaccata atgccaacga agccataaac cacggatttg
    32281 cactggtaac tgaagagcgc cgctcaacgg gaatttatgc ctatctggat atcggtttta
    32341 actcgttaat ttccaatatt cgcaactaca aaaataaagt tggtttactg gataactcgc
    32401 ggatgaaaag cgatacccag tgggtgattg attcgatgcg ggtaaaaaca ccggggcatc
    32461 ggacgcaaat tggttcgctc tccggcggta atcagcaaaa ggtgattatt ggtcgctggc
    32521 tactaacgca accagaaata ttaatgctcg atgaaccgac gcgcggtatt gatgtcggag
    32581 cgaagtttga gatctatcag ttaattgccg aactggcgaa gaaaggcaaa gggattatta
    32641 ttatctcctc tgaaatgcct gagttgttag ggataacaga ccgtattctg gtcatgagca
    32701 atggtctcgt ttccggaatt gtcgatacaa aaacaacaac gcaaaacgaa attctgcgtc
    32761 ttgcgtcttt gcacctttaa gattaggggc ttcccatgag tgcgttaaat aagaaaagtt
    32821 ttcttactta cctgaaagag ggcggtattt acgtcgttct tttagttttg ctggcaatta
    32881 ttattttcca ggacccaaca tttttaagtc tgttgaactt aagtaatatt ctcacccagt
    32941 catcggtgcg tattattatc gcgctcggtg tggcagggtt aattgtcacc caggggaccg
    33001 acctttctgc aggtcgccag gtggggctgg cggcagtagt agcggcgacg ttattgcagt
    33061 ccatggataa cgccaacaaa gtgttcccgg aaatggcgac gatgccgatt gcgctggtta
    33121 ttctgattgt ctgtgccatt ggtgcggtga tcggtttgat caacggtctg attatcgcct
    33181 atctcaacgt gacgccgttc attaccacgc tcggcacgat gatcatcgtc tatggcatca
    33241 actcgctcta ttacgacttt gtcggggcgt cgccgatttc tggtttcgac agtggcttct
    33301 ctacctttgc tcagggcttt gtcgcgctgg ggagtttccg tctctcttac atcaccttct
    33361 acgcgttgat tgcggtggcg ttcgtctggg tgttgtggaa caaaacccgc ttcggtaaga
    33421 acatttttgc cattggcggt aacccggaag cggcgaaagt atctggtgtc aacgtcggcc
    33481 tgaacctgct gatgatctac gcgttgtctg gcgtgttcta tgccttcggc gggatgttag
    33541 aagccggacg tatcggctct gccaccaaca acctcggctt tatgtatgag ttggatgcta
    33601 tcgcggcatg tgtggtgggt ggtgtatcgt tcagcggtgg tgtagggacg gtgattggcg
    33661 tggtgaccgg ggtgattatt tttaccgtca tcaactatgg cctgacgtac atcggcgtaa
    33721 acccatactg gcagtacatc atcaaagggg cgattattat cttcgccgtg gcgctggatt
    33781 cactgaaata cgcgcgtaag aaatgatgtg aatgtgggta ttgccggatg cgccgcatcc
    33841 ggcaatcggt gccaaatgcc ggatgcgtct ctggttttac aacgttaccg ggtgttcttt
    33901 ctctcccttc ttaaacttca cttctccgag atcaatacaa cccaccgggc agacgtgacc
    33961 acaaagcagg caaccaacac atttctcggt attacaatgc ggtgtacggg ttttctcgct
    34021 ccattccatc gcctggtgac cgccgtcgta acaggaaata taacagcgtc cacagccaac
    34081 acatttatcg agattgatat ggggatagac aatataactg cggtctaaat cttccgccgg
    34141 gacaatattg ttattcgcca gacctaccat ctcctgcaac gaatcaaacc cttgatcggc
    34201 gagataatgc gacaacccgc ttgccatatc ttccactatc cgatacccgt actgcataat
    34261 gccggtggtc acctgcaacg tcgctgcacc gagcaataaa aactccgccg catcttccca
    34321 ggtttcaatg ccgccgatac cgctgatcgg gaaatcgcgc agttctggat gggtacgcat
    34381 ttgctgaatg aagcgcaggg cgatcggttt taccgcttta ccggaatatc cggaaatact
    34441 cgatttaccg ttaacgatcg gcataccgat tttctgatta agatcgatat tggtgatgga
    34501 tttaacggtg ttaatcgccg caatgccatc tgcgccgccg cgcttcgccg ccagcgccac
    34561 ttcgcacata tcaccgatat tcggcgtcat cttcgccagc attggcagcg tggaaccacg
    34621 ttttactgcc cgacaatatt tttctaccag ctccgggctt tgcccgacat cgctacccat
    34681 cgcatgagaa gtcatttgcg gacaggagaa gttacactcg atcatatccg cgccagcttc
    34741 ttgcaccaga cgcgccagct cttcccattg ctgctcattt tcgcccatga ttgaagcgat
    34801 caatactttg tccgggtaat cttctttcag ccgacgcagt gcggccagat tatcttccaa
    34861 cggatgttca gcaatctgct ccatattttt gaagccgata aaaccggtat cttctttcac
    34921 cagatgatca aaacgcggcg agacttcgtt ggcgataaaa aaaccgatcg ttttaaacac
    34981 cacgccaccc cagcctgtgt cgtaggcttt ggcacacatc tcatagcagt tgcctacagg
    35041 cgaagaagag aggcagaacg ggttgggaaa cttcacacca caaaaggtaa tcgaaagatc
    35101 tttcgttaac atgagcaagc tccctttaaa tagtgatgaa tcgcctcagc ggcttctttc
    35161 ccggttttca cggcataaac cacggttttg tcaccctcaa caatatcgcc agcagcaaag
    35221 acttgcgggt cgcgggtctg gtaattttgt gttttgatgg tgttacgctg cggctctaac
    35281 tccgcaaagg catccagtct ggcatgctga ccgacggcga gaataatttt atctgccgcc
    35341 atcgtcagtt cgcccggtaa ccgtacatgc ttaaacgtga ctttattgcc ttccacagct
    35401 actggcgtga atccatcgat gatcgaaacg cccagctccc gggcgctggc aaactctttt
    35461 tcgcttgccg gaaactcatc taactcttca cgcgctacgc aagttactgc ctgacagccg
    35521 agaactttca gcgtgctggc tacgtccatc gcgacatcac cgccgccgat aattaatgcg
    35581 ctttgtggaa tgctgatatc gccttgcgcc tgtcgtgcac gttgcaagaa gtcgacggca
    35641 atctcaacat cgctatgctc aaacagtggt agccccgaac cgctcgataa ccccacagtg
    35701 accagtaccg cgcggttttc cgctttcagc tgctccaggg tgagtgtgtt accgatttcg
    35761 ttattgcatt taatggtcac gcccattttt tcaatacggg cgatttctgc atccagtact
    35821 gattgcggta accggaattg cggaatgccg ttacgcaacc agccaccggg ttgtgcttct
    35881 ttctcataga tcgtgacgtc ataaccctgg tttgtcagtg tcacactggc ctgcaatccg
    35941 gcaggacccg cgccaataat ggcgactttg ccgagcgttt tagtaccggg ctgataaatt
    36001 tccatcccag tttgttgttc aaaatcagta acaaaacgct gtaagcgacc gatatcaatg
    36061 ggagtatcaa caccggcacg ggtacaaccg ctttggcata atttctccgt cgggcacact
    36121 ctggcacaaa cggcaccgag ggcattattt tcacgaattg tctcggcagc gcctttaaaa
    36181 ttacgaaagt agattgagcg aataaatttc cccggatcgg tctgtgccgg gcaagcctga
    36241 ctacaggggg cgtcatggca taataaacag cgagaggctt ctttaatcgc cagtaacggt
    36301 gtaaaagccg gagtgagttc atccagataa ttttgttgcg gcatgtgatc ttccttaatg
    36361 ctatgagtaa ttaaggcgaa cgctaaatat actcgtcata cttcaagttg catgtgctgc
    36421 gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggggact cactcacttg
    36481 tcgccttcct gcaactcgaa ttatttagag tataaatatt gcgtgctgat attaaggcta
    36541 tgcgcgtggg gaaatttaaa ctgtgaaagg attcacaaat agctgcggtt cagaatgtta
    36601 tatccgcagc ttattgagtg aagtaataat tattctcaat aattaacaga aaagagaaaa
    36661 tattaacgat agaaaataag ataaacaatc atgccgatag ataaaattcc gaagccgata
    36721 tatgccacca tataagcgaa gaagagtccg ggattttttg ctcgccagac atacaccggt
    36781 tccggtgcat ttttaacccg tttatcaatc gcgcctgcgc gccagccttt ccatgcttcg
    36841 cggaaacaaa ataccgggat aaggaaaaaa acggcaacga caaaaaactg ctgtacatcc
    36901 ataactactt tccttgtttc ttttgtaatt caagtaactg ttcgggtgtg acggcgggat
    36961 acccctgcgc atcttcaggc acctggtgca tagccggaat agggaccagc ggccctaaaa
    37021 aacgcggttc acgtttaaaa atataaaggt cagccagcgc accgaaacgg gcggcaaatt
    37081 cgcgaatacg tactgacagc atatctttcg gtgacggtac ggcataacac tgagcctgaa
    37141 tccccatatg cagcgcaata aataatgccc gctcacagtg gaaacgttgg gtgataataa
    37201 tgaaatcatt agtatcgaaa actttgcgtg tacgcacgat ggaatccagc gtacgaaagc
    37261 ctgcgtaatc gagaacaata tctgatgggt cgacaccagc agcgattaaa tctttgcgca
    37321 tggtcatcgg ctcattataa ctttgcaatg cgttatcgcc gctcagtaat agataattta
    37381 ccttaccgct gttataggca ttaatcgctc cttgaatgcg gtagcgataa tactgattaa
    37441 ttacgccagt acgataatat tttgctgttc cgagcaccac accgacctgg cggtagggga
    37501 gatcctgcaa ttcgtcgtag atataaggcg cggttttcca gctcatccag cgatcgaggc
    37561 cgagcacagt caacagcagc aagccgatca ggactaacag gctgaggaac acgcgcttta
    37621 acatgcagga gactcgatct aaggaaaatt tccatcaggc tactttaccc gctggttttg
    37681 cgcaagaaac ggcaaactgt ttgccgggat tttcgacacc aggagagggc tggcaagccg
    37741 gacaaggcgt tcacgccgca tccggcacca ggcttaagcg agaagcacgc ggtcgatatt
    37801 gtgacagccg agagctttca gtgttgcgga ggtggcatcc cactgaatca acggcgcatc
    37861 ggctttttct gccagaaccg cgcgctggat atccgggtaa tcataaccct tgagattcaa
    37921 cagaattaac gccccctgca acggtggcag agtcgggttg aatgcggcgt tttcagcgta
    37981 gctgccgctg aaaatacgac cgtctttaca ttccagcgcg acaccgcttg gcgacttact
    38041 gtaaggcatg tgcgaacggt ttgccgccgc aatcgctgcc tgagaaagcg catcacccgt
    38101 cagcgcatag ccgtgatctt gctcgtccat cagcagcgtt ttaatctcca gatctttcgg
    38161 cccaaaggca tccggcagat agtcacgcag cgcgtgtgcc tcgcggcccg gcagatgaat
    38221 acgcagatcc agaccgctgt tcagttcatt cataaactga cggcagtgac cacaaggcgt
    38281 atagttaacg gtgatggcta caagcgcttt ttcaccactc aaccaggcgt ggctgatcgc
    38341 gctttgttcg gcatgaacgg tttgctgcat tgtcgcacca ataaattcca tattggcacc
    38401 aaaataccag gttccgctca caccgcgcgc aattgcgcca acattaaaat tcgacaatgg
    38461 cgtacgcgca caggctgccg ccagcggaag tagtgcgaat gccagcgcgt cttcgtccag
    38521 ccccgttgcg ctcttcagcg atgagacttg ctccccggtc aacaaagcgg ggaagtactt
    38581 gtctgccaga ataggttcca gtgcagattg caagttatcc gcaagttggg caaaagcggt
    38641 ttgaaaacgt ggatgcatgg cgttgcctca taatcatgta atgggataac agtttacgaa
    38701 cccgttatgg ctttatatgt gatctgaatc tcattaatta tgcaacgcgt gaaacttaca
    38761 tcaaaaatgc gcgacgcatc gcaaatttta gcccattact gccagaataa tcgggaaaag
    38821 gaacggtgcg atcagcgaag taataatccc gcataacacc agcgccagcg aactaaatgc
    38881 accttcctga taatccagct cggcgcagcg cgccgtaccg agggcgtgcg aggctgtgcc
    38941 cattgccaga ccgcgcgcag ctttggtacg aatacgcatc gcattaagca atgtatggcc
    39001 aaacacagcg ccgaggatgc cgacgaaaat cacgcaaact gcgctgattg ccggaatacc
    39061 gccaatactg ccgccaaccg ccattgcaat aggcgtggtg actgatttcg gcaggattga
    39121 cgcggcgatt tccggtgaag cgcccatcaa caatgccaca gaagtccccg tcaccatcgc
    39181 aaccacgctg ccgataaaac agatggtgat gatcgatttc cagcgcgcgc ggatctggtg
    39241 cagctgctca tataaaggat aggccagtgc gacaaccgcc ggttgaagca gatcgtttag
    39301 cacttcgcta cctttaaagt agctgtcgta agagatgccg gtcagcatta aaaaaggaat
    39361 gatcaccacc attgctacca gcaacgggtt aagcagggga aacttatacc gtgccgccag
    39421 tttgcgggcg gcaaaaaaga caatcaacgt cagcggtaat gaccaccaga tattcgccat
    39481 cattcttctg atcctttctg acctacgact ttacgttcac cgtgcaccag ttgtgaactc
    39541 cagctcacca ccagaaaaac caccagcgta ctgactgcac aggaaaccac taccggacca
    39601 aactgtgcgc ggagcaaatc aaaatattgc atgacgccta caccaatcgg cacaaacaat
    39661 agcgccatat agcgaatcag tacgtagcac cccggattga cccatttcgc cggaagaatt
    39721 tgcaaggcca gcaggacaaa caggatcagc atcccgatga tgctgcccgg aatggttacc
    39781 ggtagcaggg aagcaatgaa aatgcctgca tacaggcagg cataaatcag gacaaaagca
    39841 cgtaaatatt gccagataat gttcagtgtc ttactcataa tcaacacccc agatgagatg
    39901 gattcatcat acaattaatt cgtaaaacgt gctaccgatc acattaagaa atctcagcat
    39961 agttgaattt tagggacgta acctcgggcg agcgcccagt tcagcacact aattctgctg
    40021 taaaaagtgc agaaactgac ttaaagcata agaaggcgtt tcgttattgc gccagaacac
    40081 accgacgctg cctttctgtt ctatctttgg caggttgaga atcaccagtt gtccctggct
    40141 ggcataacgt tgcgccaggc gtaatgacaa aatagagatc atatcgctgc tttgtaacag
    40201 gttggtactg acgttcatcg acgccgactc aatcgtgttt tctggcagca tcacgccgtg
    40261 gtctaccagc gcattatcaa tgctgatgcg gatgggggta cctgtcggcc agactatcca
    40321 ccgccagtta gcaagatcat tccagctgag ggtggcgtat ttcgccagtg gatgctcagg
    40381 acgggcgaca aaacacaccg gctcggtgta gagcacgcga taattcagcg gcagctgcaa
    40441 cgcgcgaccg ccaattcgcc caaccaccac atcgacagaa cctgccaaca gatcttgcag
    40501 caatggggtc atcaccttct cttcgatatt caggtgtaga gttggcatct ccttgagcaa
    40561 atgcaaaatc gcctgggata cgcagtcagt ggcgacggga gaacagccaa ttttcagact
    40621 gccgaccagt ccaccttgtt taaaacgggc cagttcatgt tgcgagcgct ccatgtcatt
    40681 gattaaccgt tgggcatgtt gcaataacaa tttgccgcct tcagtaggac gaagtccttt
    40741 gctgtgccgc tcaaagagtg tgaggccgat ctcatcttcg agttgtgaca accatttcga
    40801 cagcgcaggc tgggtgatat tcatcatccg cgcgacatgg gtcagattgc cctgttcacc
    40861 cagcgccacc agcatttgca ggtgatgaag ctttagtttc tgcgcccagt ttgccatttg
    40921 ccataacctc caggttatct ctctgtccga aatgccattg tacatttcat ctctttaatc
    40981 acaatatcgc aacatacata aaacaaatga aacatctacc ttacctgcac tgaggcattt
    41041 actatgactc aacgacgtga tttacaagca ctgatcgatg ctgctccggt cggcaagatg
    41101 cagtggcgcg ttatcatctg ctgttttctg gtggtaatgc tcgatggttt tgacaccgcg
    41161 gctatcggct ttatcgcccc tgatattcgc actcactggc aactcaccgc aggcgatctc
    41221 gccccgcttt ttggcgcggg tctgctgggg ttaaccgcag gcgcactgct ttgtggtccg
    41281 ttgtccgatc gctttggccg caaacgggtg attgagctgt gcgtcttttt gtttggcgcg
    41341 ttaagcctcg cctcggcttt ctcaccggat ctgcaaacgc tggtattcct gcgtttcctc
    41401 accggccttg ggctgggtgg cgcgatgcca aatacaatta ccatgacctc ggaatatctg
    41461 cctgcccgcc gccgcggcgc gctggtgacg ctgatgtttt gcggctttac cctgggttcg
    41521 gcgtttggcg ggattgtcag cgcgcagctg gtgccggtga ttggctggca cgggatcctg
    41581 gtgttgggtg gcgtattacc gctgatgctg tttgttgcac tgctggtggt tctgccggaa
    41641 tcgccacgct ggcaggtgcg ccgccagcta ccccaggcgg tgattgcgaa aaccgtcagc
    41701 gccatcatcc gcgagcgtta tgtcgatacc cacttttatc tcattgaatc agcctccgtt
    41761 acaaaaggca gcattcgtca actgtttatg gggcgtcagt tacccatcac gttgatgctg
    41821 tgggtggtgt tctttatgag cttgctgatt atttacctgc tctcaagctg gatgccaacg
    41881 ctgctcaacc atcgcggcat cgacctgcaa catgcctcct gggttaccgc cgcgttccag
    41941 attggcggta cgttgggcgc gctggcgctg ggcgtgctta tggataagtt caatcctttc
    42001 cgcgtactgg cgctgagcta tgccataggc gctgtatgca tcgtgatgat tggcttaagc
    42061 caggacggat tatggctaat ggcgctggcg attttcggca ccggcattgg catcagcgga
    42121 tcgcaggtgg ggctgaatgc gctgaccgca acactttatc caacacaaag tcgcgccacg
    42181 ggcgtgagct ggtcaaacgc cgtgggccgc tgcggcgcga ttgttggctc gctgtctggc
    42241 ggcgttatga tggcgatgaa tttctctttc gacaccctgt ttttcattat cgctgtgccc
    42301 gcggcgatca gtgcggtgat gttaacccag ctgatcaccg tggttcgcca gtcgacctct
    42361 gtccctgact cactgcctca cgcaggtgtt gtgaacgaat aaggagcatc ccaatgactg
    42421 ataacaatca aaatagccgc gagcaatttt atcagcatat ttccgggcaa aacctgaccc
    42481 cgctgtggga gtcactgcac catctggtgc caaaaacgcc caacgctaac tgtgcgccag
    42541 cgtactggaa ttatcaggag atccgcccgt tgctgctgga gagcggcagc ctgattggcg
    42601 cgaaagaagc cgtgcgccgc gtgctggtgc tggaaaaccc ggcgctgcgc gggcagtctt
    42661 ccatcaccgc gactttatat gccggtttgc aactgatcat gccgggcgaa gtggcaccga
    42721 gtcatcgcca taaccagtcg gcgctgcgtt ttattgttga aggtaaaggg gcattcaccg
    42781 ccgttgatgg cgaacgcacg tcaatgaatg agggcgattt tatcctgacc ccgcagtggc
    42841 gctggcacga tcacggtaac cctggcgacg aaccggttat ctggctcgat gggctggatc
    42901 tgccattagt gaacactctg ggctgcggtt ttgccgaaga ttacccggag gagcaacaac
    42961 cagtaacgcg taaagagggc gattacctgc cgcgttacgc tgccaatatg ttgccgttgc
    43021 gccatcagac cgggaactcc tcgcccatct ttaactaccg ttatgaccgt agccgcgaag
    43081 tgctacacga tttaacccgc ctgggcgatg ccgatgagtg ggatggctac aaaatgcgct
    43141 acgtcaaccc ggtcaccggt ggctatccga tgccgtcgat gggcgctttt ctgcaactgc
    43201 tgccgaaagg gtttgcctca cgcgtggcgc gtaccactga cagcaccatc taccatgtgg
    43261 tggaaggtag cgggcaggtc actatcggca atgaaacctt cagctttagt gcaaaagata
    43321 tcttcgtggt gccgacctgg cacggcgtgt cgttccagac cacgcaagat accgttttat
    43381 ttagtttttc ggacagaccg gtacaagaag ccctggggct gttccgcgaa gcacgttatt
    43441 aatttgggag agagaaaatg actcaatacg tattcgaacc acaagcgccc gttaccgtac
    43501 cggttgttgg cagtgatgaa caattccccg tacgccgcgt ttactgcgtc ggacgtaact
    43561 atgccgccca cgcccgcgaa atgggctttg atccggatcg tgaaccgcca ttcttcttct
    43621 gcaaaccggc ggatgccatt gttccggttg ccgctggcga aacgctggcg ctgccgtatc
    43681 cggcgcagac cgataactac cattatgaaa ttgaactggt ggtggcgatt ggtaaaaaag
    43741 gcagtgatat cccgctggaa aacgcccatg aatatgtctg gggttatgcc acgggtcttg
    43801 atatgactcg ccgtgaccgt caaatggaaa tgcgccagat gggccgcccg tgggaaatcg
    43861 gcaaagcgtt cgatctctcg gcccccatcg cgccgctgca caaagccagc gaaactggcg
    43921 gtattgataa cgcacctatc tggttacagg ttaacggcga agatcatcag cgcagcgata
    43981 ttcgtcatct catctggtca gtgaatgaaa ccatcagcta cctgtcaggc ttctttgaac
    44041 tgcaaccggg cgatctaatc tttaccggta cgccggaagg ggttggctca gtagtcaaag
    44101 gtgatgtgat cacgggcaac gtcgagggct taacgcctat cgcggtgaag attgtctgag
    44161 gtgatgatga agctgtacag tttttttaat agttcagcgt cttaccgggt gcgcattgcg
    44221 ctggcgctga agggtatcga ttaccagacc gaaggggtga atatccgcat tggtcagcaa
    44281 aacgaactgg cctaccggcg gatgaatccg gtcgggctgg tgccgacgct gctgaccgac
    44341 gagggcgaat cactcggaca atcgctggcg attattgact ggctggagag tcactacccg
    44401 caagttccgc tgatgccgca ggaagaaccg gcccgcaaca aggtgctgga aattgtctat
    44461 gccatcgcct gcgatattca tccattgaat aacctacgcg tgctgcgcta cctgaccgaa
    44521 gagctgaacg tcagtgaaga agataaaaaa cgctggtatg cgcactggat ccagcaaggg
    44581 ttaagcgcgg tggaacagct actgcgccag agccagtcgg ggcaattttg tgtgggcgag
    44641 acgccgacgc tggcagattg ctgccttgtt ccacagtggg cgaacgcgct acgtatgaac
    44701 tgcgatctga gcgggtatcc gcgctgcaaa gcggtatacg acgcctgcac gcagctgcca
    44761 gcgttcattg ccgcagcgcc agaaaatcaa caagataaaa tttcagcctg accaggagaa
    44821 ccataatgac taaagtgaca cgcgcagtaa ttgtgggagg cgggatcggc ggtgcggcaa
    44881 ctgcattgtc actggcccgc caggggatca aagtggtgct gctggaaaag gcgcatgaga
    44941 tcggtgagat tggcgcaggt atccagttgg ggccaaatgc gttttcggcg ttggatagcc
    45001 tcggcgtcgg cgaaatcgcc cgccagcgcg cggtattcac cgatcacatc accatgatgg
    45061 atgcggtaaa cgccgaagaa gtggtgcgca ttgaaaccgg tcaggccttt cgcgatcatt
    45121 tcggcggccc ttacgcggtg atccaccggg ttgatattca cgcctcggtg tgggaagcgg
    45181 tactgacgca tccggacgtg gaatatcaca cctccaccaa cgtcgtggat attcgcgaga
    45241 cagaagacga tgtcaccgtt ttcgacgatc gcggcaatag ttggatcggc gatattctga
    45301 ttggctgtga cggcgtgaag tcagtggtgc gccagagctt attgggcgat gcgccgcgtg
    45361 tcaccgggca tgtggtctac cgtgctgtta tcgattgtgc cgatatgccg gaagatttgc
    45421 gcattaacgc accagtactg tgggcggggc cgtgctgtca cttggtgcat taccctttac
    45481 gcggcggcaa gcagtataac ctggtggtga cattccacag ccgccagcag gaagagtggg
    45541 gcgtgaagga tggcagtaaa gaggaagtgc tctcttactt tgaagggatt cacccccgtc
    45601 cgcgccagat gctggataaa ccgacctcct ggcgacgctg gtcaaccgcc gaccgtgaac
    45661 cggtagcgaa gtggggaacg aagcgcatta cgctggtggg cgatgcggcc catccggtgg
    45721 cgcagtatat ggcgcagggt gcctgtatgg cgctggaaga cgcggtaaca ctgggtaagg
    45781 cgctggcgca gtgtgagggt gatgcggcac aagcctttgc gctgtatgag tcggtacgta
    45841 ttccgcgcac cgcgcgcatc gtctggtcga cccgtgaaat ggggcgggtt tatcatgccg
    45901 caggagtaga gcgccaggta cgtaacctgt tgtggaaagg gaaatcgcag gcagagtttt
    45961 accgcggcat ggagtggctg tacggctgga aagaagataa ctgtttgcaa ccacgctgag
    46021 tactttaatc cgctgaccat aggcgctaac atagcgcctc atttttttgc gggtgatgat
    46081 atgcgtgtgt tactggcacc gatggagggt gtactcgact ctctggtgcg tgaattgctg
    46141 accgaagtta acgactacga tctgtgcatc accgagtttg tccgcgtggt ggatcaactg
    46201 ctgccggtaa aagtctttca tcgcatttgc cctgagctac aaaacgccag ccggacacca
    46261 gccggtacgc tggtgcgcgt gcagttgtta ggtcagttcc cacaatggct ggcagagaat
    46321 gccgcccgtg cggtcgagtt aggttcctgg ggcgtggatc tcaattgcgg ctgcccgtcg
    46381 aaaacggtta acggtagcgg cggtggggcg acgttactca aagatcctga actcatctat
    46441 cagggtgcaa aagcgatgcg cgaagctgta ccggcgcatt tgcctgtcag cgtaaaagtg
    46501 cgtctgggct gggacagcgg tgagaagaaa tttgaaatcg ccgatgcggt gcaacaggct
    46561 ggcgctacgg agctggtagt acatgggcgg acaaaagagc agggttaccg cgcggagcat
    46621 attgactggc aggcgattgg cgagattcgc cagcggttga atattcctgt gattgccaac
    46681 ggtgaaatct gggactggca gagtgcgcaa caatgcatga cgatcagcgg atgcgacgca
    46741 gtgatgattg gtcgcggggc gctcaatatt cccaacctga gccgggtggt aaaatataac
    46801 gaaccgcgaa tgccgtggcc ggaggtagtt gctttgctgc aaaaatatac ccgtctggaa
    46861 aagcagggcg ataccgggtt atatcacgtt gcgcggatta aacagtggtt gagttatttg
    46921 cgtaaagaat acgatgaagc aacggaatta tttcagcatg ttcgggtgtt gaataattcc
    46981 cctgatattg caagggctat tcaggcaatt gatatcgaga aactctaaga aaccatagtg
    47041 atgttttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    47101 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    47161 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata ag
//
LOCUS       DAZKJR010000022        43734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKJR010000022 DAZKJR010000000
VERSION     DAZKJR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.022"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            228..1322
                     /gene="gcvT"
                     /locus_tag="ACHOWS_001263"
     CDS             228..1322
                     /gene="gcvT"
                     /locus_tag="ACHOWS_001263"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFQ0046877.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1346..1735
                     /gene="gcvH"
                     /locus_tag="ACHOWS_001264"
     CDS             1346..1735
                     /gene="gcvH"
                     /locus_tag="ACHOWS_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFQ0046878.1"
                     /translation="MSNVPAELKYSKEHEWLRKEAGGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1854..4727
                     /gene="gcvP"
                     /locus_tag="ACHOWS_001265"
     CDS             1854..4727
                     /gene="gcvP"
                     /locus_tag="ACHOWS_001265"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFQ0046879.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4891..6324)
                     /gene="bglA"
                     /locus_tag="ACHOWS_001266"
     CDS             complement(4891..6324)
                     /gene="bglA"
                     /locus_tag="ACHOWS_001266"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFQ0046880.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLHEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            6369..6680
                     /gene="yqfB"
                     /locus_tag="ACHOWS_001267"
     CDS             6369..6680
                     /gene="yqfB"
                     /locus_tag="ACHOWS_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFQ0046881.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTDLKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            6844..7503
                     /gene="yqfA"
                     /locus_tag="ACHOWS_001268"
     CDS             6844..7503
                     /gene="yqfA"
                     /locus_tag="ACHOWS_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0046882.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7580..8560)
                     /gene="ygfZ"
                     /locus_tag="ACHOWS_001269"
     CDS             complement(7580..8560)
                     /gene="ygfZ"
                     /locus_tag="ACHOWS_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFQ0046883.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            8813..9079
                     /gene="sdhE"
                     /locus_tag="ACHOWS_001270"
     CDS             8813..9079
                     /gene="sdhE"
                     /locus_tag="ACHOWS_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFQ0046884.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9060..9467
                     /gene="ygfX"
                     /locus_tag="ACHOWS_001271"
     CDS             9060..9467
                     /gene="ygfX"
                     /locus_tag="ACHOWS_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFQ0046885.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9507..10028)
                     /gene="fldB"
                     /locus_tag="ACHOWS_001272"
     CDS             complement(9507..10028)
                     /gene="fldB"
                     /locus_tag="ACHOWS_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFQ0046886.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10140..11036
                     /gene="xerD"
                     /locus_tag="ACHOWS_001273"
     CDS             10140..11036
                     /gene="xerD"
                     /locus_tag="ACHOWS_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0046887.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11061..11771
                     /gene="dsbC"
                     /locus_tag="ACHOWS_001274"
     CDS             11061..11771
                     /gene="dsbC"
                     /locus_tag="ACHOWS_001274"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFQ0046888.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11777..13510
                     /gene="recJ"
                     /locus_tag="ACHOWS_001275"
     CDS             11777..13510
                     /gene="recJ"
                     /locus_tag="ACHOWS_001275"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0046889.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHSGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGVA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAL
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLTYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13601..14699
                     /gene="prfB"
                     /locus_tag="ACHOWS_001276"
     CDS             join(13601..13675,13677..14699)
                     /gene="prfB"
                     /locus_tag="ACHOWS_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0046890.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14709..16226
                     /gene="lysS"
                     /locus_tag="ACHOWS_001277"
     CDS             14709..16226
                     /gene="lysS"
                     /locus_tag="ACHOWS_001277"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0046891.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16269..16817)
                     /gene="idi"
                     /locus_tag="ACHOWS_001278"
     CDS             complement(16269..16817)
                     /gene="idi"
                     /locus_tag="ACHOWS_001278"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0046892.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            16872..16943
                     /locus_tag="ACHOWS_001279"
     CDS             16872..16943
                     /locus_tag="ACHOWS_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0046893.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16940..17065)
                     /gene="yqfG"
                     /locus_tag="ACHOWS_001280"
     CDS             complement(16940..17065)
                     /gene="yqfG"
                     /locus_tag="ACHOWS_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFQ0046894.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17067..18515)
                     /gene="uacT"
                     /locus_tag="ACHOWS_001281"
     CDS             complement(17067..18515)
                     /gene="uacT"
                     /locus_tag="ACHOWS_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFQ0046895.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18950..20869
                     /gene="ygfT"
                     /locus_tag="ACHOWS_001282"
     CDS             18950..20869
                     /gene="ygfT"
                     /locus_tag="ACHOWS_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFQ0046896.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20869..21357
                     /gene="ygfS"
                     /locus_tag="ACHOWS_001283"
     CDS             20869..21357
                     /gene="ygfS"
                     /locus_tag="ACHOWS_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFQ0046897.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVSACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(21393..22760)
                     /gene="ghxQ"
                     /locus_tag="ACHOWS_001284"
                     /gene_synonym="ygfQ"
     CDS             complement(21393..22760)
                     /gene="ghxQ"
                     /locus_tag="ACHOWS_001284"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFQ0046898.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22796..24112)
                     /gene="guaD"
                     /locus_tag="ACHOWS_001285"
     CDS             complement(22796..24112)
                     /gene="guaD"
                     /locus_tag="ACHOWS_001285"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFQ0046899.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24130..25530)
                     /gene="xanQ"
                     /locus_tag="ACHOWS_001286"
     CDS             complement(24130..25530)
                     /gene="xanQ"
                     /locus_tag="ACHOWS_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFQ0046900.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGATLQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25695..28565)
                     /gene="xdhD"
                     /locus_tag="ACHOWS_001287"
     CDS             complement(25695..28565)
                     /gene="xdhD"
                     /locus_tag="ACHOWS_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFQ0046901.1"
                     /translation="MIIHFTLNGTPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEEDNC
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28562..29341)
                     /gene="ygfM"
                     /locus_tag="ACHOWS_001288"
     CDS             complement(28562..29341)
                     /gene="ygfM"
                     /locus_tag="ACHOWS_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFQ0046902.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQTLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEEYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGDALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29392..30720)
                     /gene="ssnA"
                     /locus_tag="ACHOWS_001289"
     CDS             complement(29392..30720)
                     /gene="ssnA"
                     /locus_tag="ACHOWS_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFQ0046903.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKGGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDDAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSHSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLCDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30723..33821)
                     /gene="ygfK"
                     /locus_tag="ACHOWS_001290"
     CDS             complement(30723..33821)
                     /gene="ygfK"
                     /locus_tag="ACHOWS_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFQ0046904.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSCKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPPELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTELKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDNEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34143..34721)
                     /gene="mocA"
                     /locus_tag="ACHOWS_001291"
     CDS             complement(34143..34721)
                     /gene="mocA"
                     /locus_tag="ACHOWS_001291"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFQ0046905.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKIMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            34825..35595
                     /gene="yqeC"
                     /locus_tag="ACHOWS_001292"
     CDS             34825..35595
                     /gene="yqeC"
                     /locus_tag="ACHOWS_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFQ0046906.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRSECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            35643..37268
                     /gene="yqeB"
                     /locus_tag="ACHOWS_001293"
     CDS             35643..37268
                     /gene="yqeB"
                     /locus_tag="ACHOWS_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFQ0046907.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTECGFIPVIVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37309..38241)
                     /gene="arcC"
                     /locus_tag="ACHOWS_001294"
     CDS             complement(37309..38241)
                     /gene="arcC"
                     /locus_tag="ACHOWS_001294"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0046908.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38289..39674)
                     /gene="hyuA"
                     /locus_tag="ACHOWS_001295"
     CDS             complement(38289..39674)
                     /gene="hyuA"
                     /locus_tag="ACHOWS_001295"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFQ0046909.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQVPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(39727..40938)
                     /gene="ygeY"
                     /locus_tag="ACHOWS_001296"
     CDS             complement(39727..40938)
                     /gene="ygeY"
                     /locus_tag="ACHOWS_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFQ0046910.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(40996..42192)
                     /gene="dpaL"
                     /locus_tag="ACHOWS_001297"
     CDS             complement(40996..42192)
                     /gene="dpaL"
                     /locus_tag="ACHOWS_001297"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFQ0046911.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42250..43437)
                     /gene="ygeW"
                     /locus_tag="ACHOWS_001298"
     CDS             complement(42250..43437)
                     /gene="ygeW"
                     /locus_tag="ACHOWS_001298"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFQ0046912.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        10599 a        11006 c        11521 g        10608 t
ORIGIN      
        1 aactgatgtg ttttttggtt ttttattctg ttgcgatttt tgcatttttt aaccataagc
       61 taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta agttcaggca
      121 aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttc gtggtgataa
      181 tttcgccatg gaaaagttgt cagccccgct tattcaatga ggacaagatg gcacaacaga
      241 ctcctttgta cgaacaacac acgctttgcg gcgctcgcat ggtggatttc cacggctgga
      301 tgatgccgct gcattacggt tcgcaaatcg acgaacatca tgcggtacgt accgatgccg
      361 gaatgtttga tgtgtcacat atgaccatcg tcgatcttcg cggcagccgc actcgggagt
      421 ttctgcgtta tctgctggcg aacgatgtgg cgaaactcac caaaagcggc aaagcccttt
      481 actcggggat gttgaatgcc tctggcggtg tgatagatga cctcatcgtc tactacttta
      541 ctgaagattt cttccgcctc gttgttaact ccgccacccg cgaaaaagac ctctcctgga
      601 ttacccaaca cgctgaacct ttcggcatcg aaattaccgt tcgtgatgac ctttccatga
      661 tcgccgtaca agggccgaat gcgcaggcaa aagctgccac actgtttaat gacgcccagc
      721 gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca ggcgggcgat ctgtttattg
      781 ccaccactgg ttataccggt gaagcgggct atgaaattgc gctgcccaat gaaaaagcgg
      841 cggatttctg gcgcgcgctg gtggaagcgg gcgttaagcc atgcggcctg ggcgcgcgtg
      901 acacgctgcg tctggaagcg ggtatgaatc tttatagtca ggagatggac gaaaccatct
      961 ctcctttagc cgccaatatg ggctggacta tcgcctggga accggcagat cgtgacttca
     1021 tcggtcgtga agccctggaa gtgcagcgtg agcatggcac agaaaaactg gttggtctgg
     1081 tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt acgctttacc gatgcgcagg
     1141 gcaaccagca tgaaggcatt atcaccagcg gtactttctc cccgacgctg ggttacagca
     1201 ttgcgctggc gcgcgtgccg gaaggtattg gtgaaacagc gattgtacaa attcgcaacc
     1261 gtgaaatgcc ggttaaagtg acgaaacctg tttttgtgcg taacggtaaa gccgtcgcgt
     1321 gatttacttt tttggagatt gattgatgag caacgtacca gcagaactga aatacagcaa
     1381 agaacacgaa tggctgcgta aagaagccgg cggcacttac accgttggta tcaccgaaca
     1441 tgcccaggag ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg gcgcaacggt
     1501 tagcgcgggc gatgactgcg cggttgccga atcagtaaaa gcggcgtcag acatttatgc
     1561 gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg agcgattccc cggaactggt
     1621 gaacagcgaa ccgtatgcag gcggctggat ctttaaaatc aaagccagcg atgaaagcga
     1681 actggaatca ctgctggatg cgaccgcata cgaagcattg ttagaagacg agtaacggct
     1741 ttattcctct tctgcgggag aggatcaggg tgaggaactt ttacgcctca ccctcactct
     1801 cttcgcaagg agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac
     1861 agacgttaag ccagcttgaa aacagcggcg cttttattga acgccatatc ggaccggacg
     1921 ccgcgcaaca gcaagaaatg ctgaatgccg ttggcgcaca atcgttaaac gcgctgaccg
     1981 gccagattgt gccgaaagat attcagcttg cgaccccgcc gcaggttggc gcaccggcga
     2041 ccgaatacgc cgcgctggcg gaactcaagg ctattgccag tcgcaataaa cgcttcacgt
     2101 cttacatcgg catgggttac accgccgtgc agctaccgcc ggttattctg cgtaacatgc
     2161 tggaaaatcc gggctggtat accgcgtata ctccgtatca acctgaagtc tcccagggcc
     2221 gccttgaagc actgctcaac ttccagcagg taacgctgga tttgaccggg ctggatatgg
     2281 cctctgcctc tcttctggac gaggccactg ctgccgccga agcaatggcg atggcgaaac
     2341 gcgtcagcaa actgaaaaat gccaaccgct tcttcgttgc ttctgacgtt catccgcaaa
     2401 cgctcgatgt ggtccgtact cgtgctgaaa cctttggttt tgaagtgatt gtcgatgacg
     2461 cgcaaaaagt gctcgaccat caggacgtct tcggcgtgct gttacagcag gttggcacaa
     2521 ccggtgaaat ccacgactac actgcgctca tcagcgaact gaaatcacgc aaaattgtgg
     2581 tcagtgtcgc cgccgatata atggcgctgg tgctgttaac tgcgccgggt aaacagggcg
     2641 cggatattgt ctttggttcg gcgcaacgct tcggtgtgcc gatgggctac ggtggcccac
     2701 acgcagcatt ctttgcggcg aaagatgaat acaaacgctc aatgccgggc cgtattatcg
     2761 gtgtatcgaa agatgcagct ggcaataccg ctctgcgcat ggcgatgcag actcgcgagc
     2821 aacatattcg ccgtgagaaa gcgaactcca acatttgtac ttcccaggta ctgctggcaa
     2881 acatcgccag cctgtatgcc gtttatcacg gcccggttgg cctgaaacgt atcgctaacc
     2941 gcatccaccg tctgaccgat atcctggcgg cgggcctgca acaaaaaggt ctgaaactgc
     3001 gtcatgcgca ctatttcgac actttgtgtg tggaagtggc agacaaggcg ggcgtactgg
     3061 cgcgtgccga agcggctgaa atcaacctgc gtagcgatat cctgaacgcg gttgggatca
     3121 cccttgatga aaccaccacg cgtgaaaacg tgatgcagct tttcagcgta ctgctggggg
     3181 ataaccacgg cctggacatc gacacgctgg acaaagacgt ggctcacgac agccgctcta
     3241 ttcagcctgc gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctacc
     3301 acagcgaaac cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga
     3361 atcaggcgat gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga
     3421 tcccaatcac ctggccggaa tttgccgaac tgcacccgtt ctgcccgccg gagcaggccg
     3481 aaggttatca gcagatgatt gcgcagctgg ctgactggct ggtgaaactg accggttacg
     3541 acgccgtttg tatgcagccg aactctggcg cacagggcga atacgcgggc ctgctggcga
     3601 ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgtga tatctgcctg atcccggctt
     3661 ctgcgcacgg aactaacccc gcttctgcac atatggcagg aatgcaggtg gtggttgtgg
     3721 cgtgtgataa aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg
     3781 gcgataacct ctcctgtatc atggtgacct atccttccac ccacggcgtg tatgaagaaa
     3841 cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg
     3901 cgaacatgaa cgcccaggtc ggcatcacct caccgggctt tattggcgcg gacgtctcgc
     3961 accttaacct gcataaaact ttctgcattc cgcacggtgg cggcggccct ggtatgggac
     4021 cgatcggcgt gaaagcgcat ctggcaccgt ttgtgccagg tcatagcgtg gtacaaattg
     4081 aaggcatgtt aacccgtcag ggcgcggttt ctgcggcacc gttcggtagc gcatccattc
     4141 tgccaatcag ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc
     4201 aggtggcaat tcttaacgcc aactatattg ccagccgcct gcaggatgcc ttcccggtgc
     4261 tgtataccgg tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga
     4321 aagaagaaac cggcatcagc gaactggata ttgccaagcg tctgatcgac tacggtttcc
     4381 acgcgccgac catgtcgttc ccggtggcgg gtacgctgat ggttgaaccg actgaatctg
     4441 aaagcaaagt ggaactggat cgctttatcg acgcgatgct ggctatccgc gcagaaattg
     4501 accaggtgaa agccggtgtc tggccgctgg aagataaccc gctggtgaac gcgccgcaca
     4561 ttcagagcga actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt gcggtattcc
     4621 cggcaggcgt ggcagacaaa tactggccga cagtgaaacg tctggatgat gtttacggcg
     4681 accgtaacct gttctgctcc tgcgtaccga ttagcgaata ccagtaattc actgattcga
     4741 ctatcttcta aaggcgcttc ggcgcctttt tagcttgtta ataaagtgcg ctttgtgtgt
     4801 cggatgaagc gtaaatataa actcctggag ttcaatatat gacaggagcg ccaggcctga
     4861 tgagcggggc gcatcaggca catcgggtaa ttaaagcttc tcgccgttgc tggcaatcac
     4921 ttctttgtac cagttaaagc tcttcttacg tgaacgcgac atatcgccag taccgtcgtc
     4981 atgtttattc acatagataa agccgtagcg tttgctgtac tgcccggtag tgaacgacac
     5041 gcaatcgatg cagccccacg gtgtgtagcc catcagatcc acgccatcgt aagtcaccgc
     5101 ttttttcatc tcttcaatat gggcgcgcag gtagtcaatg cggtagtcat cgttgatgct
     5161 gccatcttct tccactttgt cataagcgcc aaaaccgttt tcgacaataa acagcggctt
     5221 ctgataacgc tcatacagtt cgcaaagtgc atagcgcaga cccacaggat caatctgcca
     5281 gccccagtca gacgctttaa catacgggtt tggtacgctg ccttcaaaac cagagatcgc
     5341 atcgccggtg ccgccttcgg ccttcaccgc gttggtcatg taatagctga aaccgagata
     5401 atcgcaggtg ccttcgcgca gcacatcgag atcgccgtct tccattttga tgttaaatcc
     5461 gcgacgctcc cactcgtgca acacatagga cgggtaatag ccacgcagtt gcacatcggt
     5521 aaagacgtag cgttcgcgca tcgactcctg ggcgaacatc acatcgtccg gattacagga
     5581 gtaagggtaa agcggcacca tcgccagcat acagccgact ttcatttccg ggttaatgcg
     5641 acgcgcggct ttcaccgcca gggcgctggc gacaaactgg tgatgcagca cctggtacat
     5701 cgtctcttcc gggttttcat gctcggtata caccacgccg gagcagcagt aaccgaacag
     5761 cggtgcacgc cagttacgct ggttgttaat ttcgttgaag gtcatccagt atttgacttt
     5821 atgcttatag cgttcaaata ccacttctgc gaaacggaca aagaaatcaa ccactttacg
     5881 gttggtccag ctaccgtatt gctgcaccag atgcagcggc atttcaaagt gggagagggt
     5941 gatcaccggt tcgatgttgt atttcagcag ttcatcgaac atatcatcgt agaacttcag
     6001 cccttcttcg tttggctgag cttcatcgcc ttttggaaaa atgcgcgtcc aggcgatgga
     6061 tgtacgaaaa catttgaagc ccatttcggc aaatagcttg atgtcttcct tgtaatgacc
     6121 ataaaaatca acggcttcat ggttcggata gtattttcct ggcaacactt ctttggtgat
     6181 ttcgcgcggc acgccgtgtg cgccaccggt cagaacgtca caaatgctcg gccctttgcc
     6241 gcctttgttc cagccgcctt cgacctgatg agcggcaact gcgccgcccc ataagaaatc
     6301 tttcggtaag gtgagttttt tcactatcat aggctccaga tgagtcaatt tttacaggaa
     6361 atagtaccat gcagccaaac gacatcactt tttttcaacg tttccaggat gacattctgg
     6421 ctgggcgtaa aaccatcacc atccgcgacg agtctgaatc gcacttcaaa acgggtgatg
     6481 tgcttcgtgt cgggcgtttt gaagacgacg gttatttttg cacgattgaa gtcaccgcaa
     6541 cctcaaccgt aacgctggat acactgacgg aaaaacatgc agagcaggaa aatatgaccc
     6601 tgactgatct gaaaaaggtc attgccgata tctatcccgg ccagacacag ttttatgtga
     6661 ttgaatttaa atgtctttaa attgaactca cgcctgttag ttgaaatttc gcatttaatt
     6721 tcatgatttt tcggagatta gtctcaaatc agaatttatt ttagctaaca ggtgttcact
     6781 ggaactattc tcagttacgc tggagaggta tacatgtgcg tgtacgtttc tccggagtaa
     6841 gttatggttc agaagcccct cattaagcag ggatattcgc tggcagagga aattgccaac
     6901 agcgtcagtc acggcattgg gttggtgttt ggtatcgttg ggctggtgtt gctactggtt
     6961 caggcggtgg atctcaatgc cagcgccaca gcgataacca gttacagcct ctatggcggc
     7021 agtatgatcc tgctgttcct cgcttcgacg ctctatcacg ccattcctca tcaacgggca
     7081 aaaatgtggc tgaagaaatt tgaccattgc gctatttacc tattgattgc cggaacctac
     7141 acgccgtttt tgctggtggg gctggattct ccgttagcgc gcgggttgat gattgttatc
     7201 tggagcctgg cattgctggg gattctgttc aaactgacca tcgcgcaccg attcaaaatt
     7261 ttatctctgg tgacctatct ggcgatgggc tggctgtcgc tggtggtgat ttatgaaatg
     7321 gcaattaagc ttgcggcggg cagcgttacc ttactggctg taggcggcgt ggtttactcg
     7381 ctcggggtga ttttctacgt ctgcaaacgc attccctaca accatgccat ctggcacggc
     7441 tttgtgctcg gcggcagcgt gtgccacttt ctggcgatct atttatatat tgggcaggcg
     7501 taatgggtgt gcctttgtag gcctgaaaag acacaccctt gtcgcatcag gcgataaatg
     7561 catgatgcga cacagagatt tactcttcga gtgaatacgg cagcggctcg atatgcaatg
     7621 tattcgcatc gtcacgcacg cggaagatgc tgtccggttc catatcgtta ttcatgacga
     7681 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcacggtt ccggtacgac
     7741 gccagttctc gcccattttc agctctaagt cttcaccggc ttccggcagt cggctggcgc
     7801 tacccgtcag caaccagagc gcgcgtttgt tggcaccacg gaatttagct cgcgccacca
     7861 tctcttgtcc ggtatagcag ccttttttaa agctaatgcc gcccagcgcc tggaggttgg
     7921 tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa cccgcttcaa
     7981 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
     8041 tcagcatatt ggcagtggct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
     8101 accatagcaa agtggtcgcg ccttctttaa ctacctgttt ttctttcgaa ggcagttcac
     8161 taaagatatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
     8221 cgtcgtctgg cgcgatggtc actttagaga acaccgcata ttttttcagt tcagtcagct
     8281 gcggttcacg cacgctgcgc cgttcaatcc acgcaaagcc atcgccgtcg cggaacagac
     8341 gtaaattgct ccacattttg cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
     8401 ctgtcatctg gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc
     8461 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
     8521 aagccgtagg ctgacgggga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
     8581 aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
     8641 gcgatctgtg ccttctgata tgctttgcga ggcgacttcc aaaaaaatga agcccttgaa
     8701 ttgttgcgaa ttaatttcgc gatcgcgatt gtgtttactg atattcccgc aaaagaagtc
     8761 gaaaaacacg ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat
     8821 taacaacaaa gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc
     8881 aatcatgccg tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt
     8941 tattcgtctg ctggaatgcg acgacccgga cctgtttaac tggctgatga atcacggtaa
     9001 accggccgat gcagaactgg aaatgatggt ccgactcatc cagacacgga accgggaacg
     9061 tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga
     9121 ttcatgggct ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt
     9181 tatggatggt gttgctttcg ctggtggtgt ttgattgcgt tcgcagccag cgccgcatta
     9241 atgctcgcca gggggaaatt cgcctgttga tggacgggcg tttgcgttgg caagggcagg
     9301 agtggagcat cgtcaaagcg ccgtggatga ttaagagcgg catgatgctg cgtttacgtt
     9361 ctgacagcgg taaacggcaa catttatggc tggcagccga cagcatggac gaagctgaat
     9421 ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa
     9481 gcatatgcgc taagccgtca gtgacatcag gcgtaatgct ctgccatttc gttgaggatt
     9541 tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc
     9601 gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat
     9661 ccttccgttg gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc
     9721 ataccgagcg catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc
     9781 gcaacaattt taccttcgag gttcaggtcg tcgagctgat cccagacggc ttcccagtct
     9841 tcctggattt cgccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc
     9901 attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata
     9961 tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc
    10021 atattcataa cgtaagcttc tcgattctgc tgtggctttt gcggctagtg tatcagaatc
    10081 gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag
    10141 tgaaacagga tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc
    10201 tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc
    10261 atcaccgtgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag
    10321 aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc
    10381 gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga tgatcccagt gcgcatctcg
    10441 cttcaccgaa attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt
    10501 tattacaggc accattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag
    10561 tgttgtatgc taccggactg cgtgtctctg aactggtcgg gctgacaatg agtgatatca
    10621 gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt
    10681 taggtgaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgt
    10741 tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac
    10801 agaccttctg gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc
    10861 tgtcaccgca tgtgttgcgt cacgcttttg ccactcactt attaaatcat ggtgcggatt
    10921 tacgcgtggt gcagatgcta ctgggccaca gcgatctctc caccacgcaa atttatacgc
    10981 atgtcgctac cgagcgtctg cgacaacttc atcaacagca tcacccgcgg gcgtgatgtc
    11041 tgaaaagaac aggaaaattt atgaagaaag gttttatgtt atttaccttg ttggcagcgt
    11101 tttcaggctt tgttcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca
    11161 tcaaaagcag cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca
    11221 gcggtgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg
    11281 ttagtggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc
    11341 ttgaaaaaga gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta
    11401 ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc
    11461 tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga
    11521 aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg
    11581 caggtaaaag cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg
    11641 gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc
    11701 cgggttacca gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca
    11761 gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgagaag tcgatgaaac
    11821 ggccgacttg cccgctgaat tgcctccctt gctgcgccgt ttatatgcca gccggggtgt
    11881 gcgtagcgcg caggaactgg aacgcagtgt taaaggcatg ctgccctggc agcaactgag
    11941 cggcgtcgaa aaggccgttg agatccttta caacgctttt cgcgaaggca cgcggattat
    12001 tgtggtcggt gatttcgacg ccgacggtgc caccagcacg gctctaagcg tgctggcgat
    12061 gcgctcgctt ggttgcagca atatcgacta cttggttcca aaccgtttcg aagacggtta
    12121 cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac
    12181 ggtggataac ggtatttcct cccattcggg cgttgaacac gctcgctcgt tgggcattcc
    12241 ggttattgtt accgatcacc atttgccggg cgaaacatta cccgcggcgg aagcgatcat
    12301 taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc
    12361 gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg
    12421 cggcgtcgca attcctaacc tggcagaact gctggatctg gtcgcgctgg gaacagtggc
    12481 ggacgtcgtg ccgctggacg ccaataaccg catcctcacc tggcagggga tgagtcgcat
    12541 ccgtgccgga aagtgccgtc cagggattaa agcgctgctg gaagtggcaa accgtgatgc
    12601 actaaaactc gccgccagcg atttaggttt tgcgctgggg ccacgtctca atgctgccgg
    12661 gcgactggac gatatgtccg tcggtgtggc gctgttgctg tgcgacaaca tcggcgaagc
    12721 gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg
    12781 aatgcagatt gaagccctga ccctgtgcga gaaactggag cgcagccgcg acacgttacc
    12841 cggcgggctg gcaatgtacc accccgaatg gcatcagggc gttgtcggta ttctggcttc
    12901 gcgcatcaaa gagcgttttc accgtccggt tatcgcgttt gcgccagcag gtgacggtac
    12961 gctgaaaggt tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt
    13021 agacacactc taccccggca tgatgctcaa gtttggcggc catgcgatgg cggcgggttt
    13081 gtcgctggaa gaggataaat tcgaactctt tcaacaacgg tttggcgagc tggttaccga
    13141 gtggctggat ccttcactat tgcaaggcga agtggtgtca gacggtccgt taagcccggc
    13201 cgaaatgact atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt
    13261 cccggagccg ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgagcg
    13321 tcatttgaaa gtcatggtcg agccggtcgg cggcggtccg ctgctggatg gtattgcttt
    13381 taatgtcgat accgccctct ggccggataa cggcgtgcgc gaagtgcaac tgacttacaa
    13441 gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg
    13501 gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt
    13561 atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt
    13621 aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact
    13681 acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct
    13741 ggaacgaacc cgaacgcgca caggccctgg gtaaagagcg ttcttccctc gaagccgttg
    13801 tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg
    13861 ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgctctgg
    13921 aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg
    13981 actgctacct cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca
    14041 tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg
    14101 aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt
    14161 acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aagagcccgt
    14221 ttgactccgg cggtcgtcgc cacacgtcgt tcagctccgc gtttgtttac ccggaagttg
    14281 atgacgatat cgatatcgaa atcaatccgg cggatctgcg catcgacgtt tatcgcgcgt
    14341 ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt actcacatcc
    14401 caaccgggat cgtgacccag tgccagaacg accgttccca gcacaagaac aaagaccagg
    14461 ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga
    14521 agcaggcgat ggaagacaat aaatccgaca tcggctgggg cagccagatt cgttcttatg
    14581 tccttgatga ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg
    14641 ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag
    14701 gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg
    14761 aactgaaaac gcgtcgtgag aagctggcga acctgcgcga gcaggggatt gccttcccga
    14821 acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga
    14881 acgaagaact ggaagcgctg aacatcgaag ttgccgttgc tggccgcatg atgacccgtc
    14941 gtattatggg taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt
    15001 acgttgcccg tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc
    15061 tcggcgatat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta
    15121 tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc
    15181 acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg
    15241 atgaatcccg caacaccttt aaagtgcgct cgcagatcct ctctggtatt cgccagttca
    15301 tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc cctggcggtg
    15361 ccgctgcgcg tccgttcatc acccaccata atgcgctgga tctcgacatg tacctgcgta
    15421 tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa
    15481 tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga
    15541 tggaactcta tatggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt
    15601 tccgtactct ggcacaggat attctcggta agacggaagt gacctacggc gacgtaacgc
    15661 tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc
    15721 cggaaaccga catggcggat ctggacaact tcgactctgc gaaagcgatt gctgaatcta
    15781 tcggcatcca cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag
    15841 aagtggcaga agcacatctg atccagccga ccttcattac tgaatatccg gcagaagttt
    15901 ctcctctggc gcgtcgtaac gacgttaacc cggaaatcac agaccgcttt gagttcttca
    15961 ttggtgggcg tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc
    16021 agcgcttcct ggatcaggtt gccgcgaaag atgcaggtga cgacgaagcg atgttctacg
    16081 acgaagatta cgtcaccgca ctggaacacg gcttaccgcc gacagcaggt ctgggaattg
    16141 gtatcgaccg tatggtaatg ctgttcacca acagtcatac catccgcgac gttattctgt
    16201 tcccggcgat gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc
    16261 ggggtttttt aattgtgctg cgcgaaagca gacaacaatt ttcttgcttc actattggct
    16321 gcctgcatca ccatccacgg actgaacgcc cacggcgtgg catcaatacc gtgtaataca
    16381 tctgctaaat cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg
    16441 gtggtgcgtg cggcaaatac cggacacact tcattttcca caatgccatt cggatcggtg
    16501 gcgcgatagt agaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca
    16561 taacggcaac ggcggatcac cgcgtcttcg ttgctttctc ccagttgtgg gtgcccacaa
    16621 accgagttag tccacacgcc aggccacgct tttttgctaa gggcgcggcg ggtaactaat
    16681 aattgccctt tggcattaaa cagccaactg gagaacgcga gatgtaagag agtgtctgcc
    16741 gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg
    16801 acgtgttccg tttgcataat ttctcacatg taattctaat cactcatcat tatagataat
    16861 tgaagagtga aatgattaac caagtgagcg tttatcgaca accgcccgtt ttgagcggat
    16921 gtcgacaggt aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg
    16981 ccattgcaac acagtcagaa atgacaggaa tagagagagt aaaaagcaac agcagactga
    17041 atatagcgcg cattaaaaga ttcatattat tctccatgct catttttctt cagcttgcgc
    17101 atcagaagcc acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc
    17161 accagatcag catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg
    17221 gttgcgagca taatgccgct atgcagcaac ggttgcagta cggccggtaa cttagaaaag
    17281 aaatcgtgag agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg
    17341 ttgtaacggt tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc
    17401 atgccgaaca tcaccagacc agcgccgccc agcacaaatt gcggaatgga ggcgaccagc
    17461 accgccattt ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc
    17521 cagcggctat gaacgcgcgt cacgctaacc aggccaacgt tttgagaaaa tgacgtatgg
    17581 gggaagctgt taaacgttcc gcctatcatt gtccctacgc catcgacacg cagcccgcga
    17641 ataatatcgt gtgaagagag tttacgacca actatttcac ccagtgccag gaacatcccc
    17701 atcgactcga taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatca
    17761 aaaatcggca taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc
    17821 ccggataaat tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt
    17881 aatacggcga cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt
    17941 aagacggcaa aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct
    18001 cctgcggccc agtcaatacc cacctgaatg atgctaagtc cgatagaagt aataaccaca
    18061 ccggtaacca gtggcgggaa taaaggcatc aagcgaccga taagtggcgc taataatgtg
    18121 gtgataaaac ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc
    18181 atcccaatgg ctatcattgg tgttacagcg gcgaaggtca ccgacataat caccggcagg
    18241 cggatcccca taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa
    18301 aagagatccg agctaatgag catcgcaata gcttcttttg agaggcccag tcggtcgcca
    18361 atcataagag gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt
    18421 atgatcagct ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca
    18481 gaagatgagg gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag
    18541 agtaaaaagc aaccgtttac gttcatgaaa ctgtctaaat acatgctcgt ggagtgccag
    18601 ttttgcgagg aaggggagtt agagacagga attgcggagg taatcacata atcgtggtta
    18661 aaccacgaaa atgagaagag aacctgacaa tggaattatc tgtaattttc tcatattgat
    18721 atttcattaa tcattctggc atgtcgcact ctcgcattta atcgttttta tctggatagc
    18781 attcttttga tcggcaatgt tgaaataatg aaagctgcat agattgaggg gcaataattt
    18841 gaactcagtc gcaaaattat aattaaatcg gccatgctac ctaatacatg cccatttatt
    18901 gcatcgcgaa atttgagaag acataactca ttaaatgaaa gggatgcaaa tgaataagtt
    18961 tatcgctgct gaagctgcgg aatgtatagg ctgtcatgct tgtgaaattg cctgtgcgat
    19021 ggcacataat caagaaaact ggccgctgag tcacagtgac tttcgaccgc gtatccacgt
    19081 tgtagggaaa ggccaggctg cgaatccggt ggcctgccat cactgcaaca atgccccttg
    19141 cgttacggct tgtccggtta atgctctgac tttccagccc gatagcgtac aactggacga
    19201 gcaaaaatgt attggttgta aaagatgcgc aatcgcttgc ccctttggcg tcgttgagat
    19261 ggtcgatacg attgcgcaga aatgcgacct ttgtaaccag cgcagttccg gcacgcaagc
    19321 ctgtattgaa gtctgcccaa cacaggcgtt acggctgatg gacgataaag ggttgcagca
    19381 gataaaggtg gcccgccagc gcaaaacggc agcaggaaaa gcgtcatcag acgctcagcc
    19441 atctcgcagt gcagcgttgc tccccgttaa ctcgcgtaaa ggcgcagata aaatttcagc
    19501 gagtgaacgg aaaacccact ttggcgaaat ctattacggg ctggatccac aacaagcgac
    19561 ttatgagagt gaccgctgtg tttattgcgc cgaaaaagct aactgcaact ggcattgccc
    19621 gctgcataac gctattccgg attacatccg tctggtgcag gaaggaaaga ttattgaagc
    19681 ggcagaactt tgccaccaga ccagttcctt gccagaaatc tgcggcaggg tatgtccaca
    19741 ggaccgtctt tgtgaaggcg catgtacttt gaaagatcac tctggcgcag tctctatcgg
    19801 gaatctggaa cgctacatta ccgataccgc gctggcgatg ggctggcgtc ccgatgtcag
    19861 caaagttgtt ccccgtatcg aaaaagtggc ggtgattggc gcagggcctg cagggttagg
    19921 gtgtgctgat attttggcgc gcgcaggagt tcaggttgat gtctttgatc gccatccaga
    19981 aattggcggt atgctgactt ttggcatacc tcctttcaaa ctcgataaaa cggtattaag
    20041 ccagcggcga gagatattca ccgcaatggg aatcgacttt catcttaact gtgaaattgg
    20101 ccgcgatatt acctttagcg atttaacttc tgaatatgat gcagttttta tcggcgtggg
    20161 gacttacggg atgatgcgag cagatctgcc gcatgaagat gcgcccggtg tcattcaggc
    20221 gttaccgttc ctgactgccc atacccgcca gctcatgggg ttgccggagt ctgaagagta
    20281 tccgctgacg gatgtggaag gtaagcgagt cgtggtattg ggcggtggcg atacgaccat
    20341 ggattgtttg cggacttcca tccgcctcaa tgccgccagc gtgacctgcg cgtatcgtcg
    20401 tgatgaagtc agtatgccgg gctcgcgcaa agaggtggtc aatgcgcgcg aggaaggtgt
    20461 cgagtttcag ttcaatgttc agccgcaata tatcgcttgt gacgaagatg ggcgtttaac
    20521 tgcggtgggc ctgattcgta ccgccatggg tgagccgggg ccggatggtc gccgtcgtcc
    20581 tcgtccggta gcgggttcag agtttgaatt gcccgccgat gttctcatta tggcctttgg
    20641 tttccaggcg catgccatgc cgtggttgca gggcagcgga attaaactcg ataaatgggg
    20701 cctgattcaa accggtgacg tcgggtattt acctacccag acgcatctga aaaaagtctt
    20761 tgctggtggt gatgctgttc atggcgcgga tctggttgtc actgcaatgg ccgcaggaag
    20821 gcaggcggcg cgcgatatgt taactctgtt tgatacgaag gcatcatgat gaaatcgtta
    20881 attatcgtta atccggctga ctgtattggc tgccgtactt gtgaagtagc ctgtgtggtc
    20941 gctcaccctt cagaacagga gttgaatgcc gatgtctttt taccccggct gaaggtacag
    21001 cgactggaca gcatcagtgc gccggtgatg tgccatcagt gtgaaaacgc cccttgtgtt
    21061 agcgcttgcc ccgtgggggc gctgacgatg ggcgagcagg tggtgcagac caattctgcc
    21121 cgttgtattg gctgtcagag ttgcgtcagt gcgtgcccgt ttggaatgat caccattcag
    21181 tcgttgccgg gggatacccg gcaacaaatc gtgaaatgcg atctctgtga acagcgggaa
    21241 gaggggccag cctgcgttga atcttgccca acacaggcgc tgcagttgct tactgaaaga
    21301 gaactcaggc gagtccgcca gcagcgtatt gttgccagcg gtgagaatcc tctgtgaaag
    21361 aaatctccgc gctgaaaggc gcggaggatc tattagattg cccaaccacc cgcgtaaaat
    21421 gcgaccagtg cggcagtaat gatcaccgta ccaatattca gcttttgcca ttcgcgtgca
    21481 aagacgcggc ctacgaccag tgtcacaaag ccaagcataa taccggtaac gatattacaa
    21541 gtcagaacga tgaacacggc acacaccagg ccagccattg cgtcaataaa atcattgaaa
    21601 tccagcttcg agacgttact taacatcagc aaacctacgt acatcagagc gggggcagtg
    21661 gcgtaaccag ggatcaaaaa tgacagcggt gataagaaca gaatcagcag gaataacgcc
    21721 cccactacgg ttgcggttaa accggttttc cccccggcgg cggttcctgc cgctgattcg
    21781 atataaaccg ccgcgggcgc tgcgcccacc aggccggaga atattgaact tactgagtca
    21841 ctggtcaggg ctttgccgcc gttgatgatc tggttgtctt tatccaacaa attcgcctga
    21901 ccggcgacgg cacggatggt gccagtggcg tcgaacactg cggtcatcac caatgccagt
    21961 acactcggaa gtacagttgg ctggagtgcg cccataatat cgaggctgaa aatcagagac
    22021 ttaccatctt cgccagtcag gcttggcatc gccaccagac cgtggtactt caccgctggg
    22081 tcaaagatta agccgatgat cgaaattgca ataatcacca acaagatccc gccgggtacg
    22141 cgacacttct ccagaccgaa gataaccgcc agccccagca agctcatcat caccgggaag
    22201 gaggtaaacg caccgagcgc cactggcaag ccttcaatcg ggtttttgat aaccattccc
    22261 acaccgttag cagcaatcag cagcagaaac aggccgatac cgatacctgt accgtgagca
    22321 atccccatcg gcaaattacg taagatccag gtacgcacac cggttacgga aatggcggtg
    22381 aagatgacgc ccatcagaaa taccgcgccc agtgcgacag gaacgctaat ttgttgcccg
    22441 agtaccagac tgaatgcggt aaacgccgtc aaggaaatcg cacaaccaat cgccattggc
    22501 aaattggccc acaaccccat cagcaacgag ccgaagcccg cgaccagaca ggtggcaaca
    22561 aacacagctg cgggaggaaa acctgctttg cccagcattc ccggaacgac gataacggaa
    22621 taaaccatgg ccagaaaggt cgttaagcca gccagtactt cctgacgaac ggtactgcca
    22681 cgagcggtaa ttttaaaata attatccagc gcgccctgtg gctttggtgc gtccggtgtt
    22741 tgtaggatgt ctccagacat aatgatgtcc tcacgaggat gttaaagttt tattattagt
    22801 tgcgttcgta caccagacga ccatcaacgt aggtgcggta gatcgaacgg tcatcgccca
    22861 acgtcatcat cacgaacaat ttgtcgacta aagaaacaga gttgtcatag cgcagctgct
    22921 gtagcggagt ggcagtgggt tccatcacca cgaaatcagc ctctttgcca ggtaaaaagt
    22981 tgccaatcaa atcgtcaagg cccagagatt tcgctccgcc gagcgtggcc aggtaaaacg
    23041 cttcatatgc cgagaggcga tagccttgta attgcaatac cttgtaggct tcgttcagcg
    23101 tttgcagcat gttaaaagtt gttccggcac cgatatccgt tcccatgccc actttgactt
    23161 tcttctgcca tgcttttttc aagttgaata agccgctgcc gaggtaaagg ttggaggtcg
    23221 gacagaaagc aatgctggat ttggtttcgc tgagacgatc ccactctttt tcttcgagat
    23281 ggacgcagtg agcaaagaca cagtttttac cggtcagacc gtactgatgg taaacatcca
    23341 gataaccatc atggtcagga taaagcgatt tgacccaggc aatttcatct ttgttttcac
    23401 agagatgggt atgtacccac gtatccggat attcttcttt caggcgttgc gccatcgcca
    23461 tctgttcagg agatgaggta ggggcgaagc gtggcgtaat cgcatacaac agacgaccat
    23521 ttttgtgcca gcgttcaatc agttctttgc tttggtgata gctgctttcg gcagtgtcga
    23581 gcagataatc cggtgcgttt cggtccatca tcaccttacc ggcaatcata cgcatattga
    23641 tatgactggc ggcttcaaac agcgcatcaa cggattgtgg atgaacagtg ccaaacacca
    23701 gcgccgtggt ggttccgtta cgtaaaagct gcttgatgaa gaacgccgac atctcgcggg
    23761 cgtactctaa atcctcataa cgacgttcag tagggaaggt gtgtttattc aaccactcca
    23821 gcaattgttc accataggcc cccaccattt cactttgcgg ataatggata tgtgtatcga
    23881 caaagcccgg tactatcagt ttgccgcgat agtcgcgcac gcgaatggtg tcaggaattt
    23941 gatgctttcc gtcttcccat tcgccaaacc attccacttt tccctgttta atgagtaata
    24001 aaccatcctc aataaaccgc agcgcagagg caatttcttc cgggttatcg accgtacggg
    24061 tgacatcaat aaaactgcct cgtaccgctt ttaacgtgtg ttctcctgac atcattgact
    24121 cctttactgt taatccatct cttccgctga ggtaatacca ggcagaacgt tttcctgtcg
    24181 gtagccacca gggaggataa tatttaataa aatcgcagtt aacccgccag cacaaatagg
    24241 gttttcaact aatacataaa tagaggctgg caatatttta aaaatttcgg gatcgtagga
    24301 gacgccaagc cctaaaccta aagacgtggc gacaataagt gtttctcggc gctttaatcc
    24361 gttggtgatg atgatgcgaa tcccagcgat ggcaatcatg gaaaacatca acgtcattgc
    24421 gcctcccaga actgccgagg gaatggtcgt gaagaagccg ccaatcatcg gaaataagcc
    24481 gaggataacc agcattaccg cgatggttcg cccgacataa cgtgaagcga cgccagtcat
    24541 ctgaataacc ccattatttt gcgcaaacgt ggttaatggt aatgaaccga cagcggaggc
    24601 gataacagaa accagaccat ctgccagcac gccgcctttc agtcgggact gatactcttc
    24661 cccctgaatg gggcggcggg aaaccattgc cgtggcggtg atatcgccga ctgcttccag
    24721 cacgctaagc agataaatcg tgccaaccac caggaactga tggaagctaa aactaaagcc
    24781 gtatttgaac ggatgcggga tggtgattaa cggcaaattg cgcatactgc tgaaatccac
    24841 catgcccagg cataacgatg cgatatagcc gacacatagc ccaatggcga tccctcccat
    24901 gcgtagcaac ggactgcgac agcagttaaa gccgatcacc acaattaaaa ccaataaacc
    24961 aacgccgaga tgttcgtaat tgccgaacgt accgctgctt ttggctgcaa atcctccacc
    25021 aaaatcgata atgccgactt taatcaggct taagccgatc atcagtacca caataccgct
    25081 gacggtaggc gtaataaccc gacgtaaata gggcaggata aaagaagagc cgacaaccag
    25141 aaatgcgcca acgaaggaga cgccgaggag cgacgacatg attaactctt cgtgaaaacc
    25201 gtcgcttttc atgctgctgc ccagcgcaat catgaccgta acaaatgaaa aattgactga
    25261 ctgaattgag agaagaccag aaccgacgat gccgtagcgg tttacttgta accaggtacc
    25321 aataccagag gcgatcatcg ccatagaaac aagataggca gttgtttcag cggaaagctg
    25381 taaggtcgca cccacgatta acgcgggggt taccatcgga acgaaaattg ccaacagatg
    25441 ggtaatggca cctacgagag cctgatgaaa gggagggcga tcctccagtt caaatataag
    25501 gtcagaacct gcatggttta tatcagacat cccttcactc cttttaaagt aaatttgatc
    25561 atctgaatta gtaaaataaa ttcccacgaa tttggggcgc aggacgcctt gagaacggct
    25621 tatctcattt tcgcagtcac tatttagtca tcgttcaaac agagaaacag gccgccgaag
    25681 cggccattta ttacttatat tttttccagc gcggtgagta ttttctccgg tgtgaaatgc
    25741 cattcgcgta accagatgcc gcaggcatcg tgaattgcgg tagcaatcgc cggagcggcg
    25801 ccatttacac cgatttccga gatcgatttc gccccgaatg ggccgacttt atcgtcgctc
    25861 ggcaccagca cagcgcggaa atcacgcgga atgtcaccaa ttttcggtgc gccgtaactg
    25921 cgtaaatcac gcgttaacgg gtgaccttcg gcgtcataaa tgatctcttc gctcatactg
    25981 tggccgatag ctcgcagagt ggcaccgtag atttgtccca acgctaactc tggattgacc
    26041 ggtgtaccgc agtccagcaa ggcgtagaat ttatccaggc ggatttcacc cgtacgcgtg
    26101 ttgacggcaa cttcagcgaa gtttgcgcca tacgggaagg cgaaatcagg cgtgatataa
    26161 ctgccagttc ccaccagtga accaaagccg gtgccggttt cgcctttatg ggctatctct
    26221 ccgaaactaa cttcgccttt cttgccgcgt acgacgcccg gcgttgctag ttggacatct
    26281 gccactggct cacccaacat ttgcgcaccg tggaacagaa ttttctcccg cagattttct
    26341 gctgccaaac gcgccgcgtt acccgagaag caagtaccgg acgaggcata tgcgccttta
    26401 tcaaacaacg catgatcggt atcaccggag ataacatgca cgtcctgcgg tgggcagtgc
    26461 agcacttctg ctgccagttt cgtcaccaca gtatccagac cagtaccaat atccgcaccg
    26521 ccagaatgaa cgataaaggt accgtctgat tccagtttga tcatgcagtt agcctgatcg
    26581 atatctggga tccctgattt ctgcatgata atggcgacgc cacgaccgat gtgccagtca
    26641 ccgttttgtg gttttggtga agaccattgg atcatctcgc gaccctgacg caggatctct
    26701 tccagtgcgc agctggcggc ggaaggaacg gaggtcggcg ctttaccttc accgattgca
    26761 ccgagaattt tcagctcttg cccttcgtgt acccggttac gttcgataat ttccagttgg
    26821 tcgatctgta actgttcagc cagttccgct aatgccatgg tgatagcgaa gttacctttc
    26881 ggtgcgccat aaccctgata agcaccattt gggcaaatgt tgctgtagta ggtggtgacc
    26941 tggaaatcga cgttatcgca cggatataac ggcagcgaca gcgccggtcc gttacatggt
    27001 acggtgagtg agtggttgcc gtaagggcca gtgttggcgc ggaaatccat cttcactgct
    27061 gtcaggcgac catctctttt cgctcccagt ttgacggtga ctttcgcaac gtgacgagag
    27121 gtgttagcaa taaactcttc ttcacgggtg tagcggaaca gtaccggacg cccggtcacg
    27181 caggttgccc aggcgcacac ttcttccagc aggatgtcct gtttagaccc aaaaccgccg
    27241 ccaactcgct ctttaatgac atgaacttta tgttgtttca tgcccacgag gcgcgcgacc
    27301 tggcggcgta agtgccatgg tacctgggtg gaggcgtgga tcaccagacg atcgccgtcc
    27361 atacgggtaa agcagatatg tgtttcagtc gggcactgct gtgcctgcgt tgagttatag
    27421 gttcgctcaa tgatcacatc ggcatcggca aagcctttgt ccatatcacc aatatgacca
    27481 tgaatactgg cagcgatatt tttgcgtggg cgagaaccga tcgggaagtt gatgatcata
    27541 tgctcgccgc gctgggctgc atgacagtta tcttcctcca gagtatctgg tgcaccagca
    27601 acatacacca ccggttcatc gtgcacgaca ggcgcatctt ccgccattgc ctcgtcaatc
    27661 gacattaccg gcttaagcac ttcatattca acgtcgatga gcttcagtgc ttcgagcgca
    27721 atgtcttcac tttcagcgac gaccgcagca acgcgatcgc cgacgtgacg cattttcttg
    27781 ccgaacattc ggcggtcaag cggtgacggt tccggtgcgc tctgaccacc cggggtatag
    27841 taaatatccg ggcaattcag gtgagtaata acgtgaacga cgcccggtaa ggcttcagct
    27901 ttgctgacat ccagatgagt aatcagtgcg tgagcgtgtg ggctacgtaa cattttaatg
    27961 acgcaggcgt cagccgttac gcgatcttca acatagcagg gtttcgcctg caccattttc
    28021 gcggcatcag ttttaggata atgcttgcca atgacttcaa ggtcgtcacg gaaagtcgga
    28081 gcgatatcaa tggtggcctg cggattattt ttacgtgcaa ccgccagttc aatgacctga
    28141 tagtattgct gccagccagc atcgcggcta aacagacctg agagcgcgtc gtcgatctct
    28201 tcgcgggtag gtgcgtcgat gcgatcgaga agatcggtga taatcagagc cgcagctgga
    28261 tcgttataac cagactgcac cacgccaaca tcaaccatgg cctgttgaac cagacttaac
    28321 tcattccatt tgcccagaga ttctgcggta cgaatatctg ccttctctaa ctgtgcggcg
    28381 ataagcaagg acgcgttaac gatattaccg ttaaagataa ttgcgtcaga accggcaaac
    28441 ccaaagccat catcgctgtt gcgtacagag tgcattccca tgttaaacaa cagcttttgc
    28501 acgttttcac caggattaac ggttagctcc tggggcgtgc catttaaagt aaagtggatg
    28561 atcatacggc ttcctcccca gcctgttggc agtcggcata aaggtcggct accagaactc
    28621 ccgtgatata gcgtttatag gccacgctgc cccgcaaatc ttcctgcggg aaaatggcgt
    28681 tggcgacagc ctgttcaagg gcatcacctt ccaggttttg tttttcaaca tcgtgcagac
    28741 gcagtgcttt actggctacg ccatccagcg caattcgcat accgtcgtgg tctgttattg
    28801 caacggctgc cgtcacgacg gttaaacctg cctgagaacg gctaattttg cgagtcgcac
    28861 aggtgcgata cggatcttta atgatgattt cggttagcag gcgatcgcat gggcaggcca
    28921 ggtactcctc aattgacaga gtttcgccgt tgccaaaaac cagttcagca tccagtgcca
    28981 gcaggacggg aagcagcact gactcttctt gccgggcggc aatttcacca ccaatggtcg
    29041 actgattacg aacatggcgt gagtaaacaa aaccgagagc ttcacgcagt gctgcaggaa
    29101 taaatcgcgc atcacgcagt gtttgcaagc gagacatcgc gccaatccgc agtgcaccgt
    29161 tatcccagtc aacccagtcc agttccagat cctgtaagga aatggcaatc tttttatcgg
    29221 tacgggttgg tgtagcgttg agtttgctgc ccccggcgaa ccagacggct tcatcctggt
    29281 agcggcgctt cagttccagc gcctgttcga cagagtcggg tctgaaaaat tgttcaatca
    29341 tcgtctttcc ttaatttccc ctcttctctg aaagaagagg ggagctgtta tttatgccag
    29401 cgcatccatc cgacgccaca tactggcagc ggcttttctg gcttgcgcat aaatggaatc
    29461 gcaatcgaag ttaaactgac ggtcttcata gaccatcaca ccattgacca tcacgctgtg
    29521 aacgctgcct gagcccatac cgaaagcgat atgcccggcg atattgtctg ccagcagcgg
    29581 cgtcggcgag ttgtaatcgc aaatggttaa atcggctttg taaccggctt ccagaaggcc
    29641 aaatttcgcg ccaaagttgc ggctcatcag ttcgttgccg ttagccagag ctttggcaaa
    29701 gctgtcaggc cacagcggac cacccgcatc gcgatgttta aagaaggcaa atttcatctc
    29761 ttcaaacata tctgaaccaa taccgtccgt acccaacgcc agattgcgga tgtcgcaaag
    29821 atgatggttg tagccgacat ggttgttcat gtttgaacgg gcgttatgca ccaggaatgc
    29881 atcacgctga ttgagcaacg cgatgtcgtc tttagacaga taaagcccat gagcgaccag
    29941 cgttttgctg tcgatgagat cgaattgcgc taatcgtgcc agcagatctt tgccgtacca
    30001 gtgatgacta tgggaaacat cgtaaaggtc ttccgcagcg tgaatatgta aaccacggcc
    30061 tgtggctttc acggcctcac gcagcatttc cagaccggca tccggcacag taaacggcgc
    30121 gtgagcacca atatgagcct ctaccagata cggttcagtt gccgctttct tcgcatcatc
    30181 aatctgacgg gcgaagcgga tgttttcttc tacaccttcc tgcaactctt tgataccgtt
    30241 gttacggtca gtagtttcaa aacaggtcat cgcgcgcagg ccaactttta aaaatgcgtt
    30301 gcgcaatgtg gagagcgacc cgtcgatata cgccggagag gcatggtgat cgataaccga
    30361 tgtacatccg cccttaatcg cttccaggga acaaatcagt ccgctgtaat agagcgactc
    30421 ttcatcaagg gcgcgatcga gtcgccacca gagatttttc agcgttgaga tgaaatccgg
    30481 gcagggggcg atgtttgcca taattccgcg ggacagcccc gagtaaaaat ggttgtgcga
    30541 gcagacaatt cctggcatca caatccgacc atgcatctct ttgtagctgg catcggggta
    30601 gcgttgcgtc agggcatcgc cgatagcgac aatcacatca ttttcgatgg cgatatcaac
    30661 gccttcctgc actttcgctg ggtgtaactg cactgcggtg acattcttca gaatcaacat
    30721 gattacacct ccacacggcc cagcagataa tgatgatgct ggtggacatg gctaatgatg
    30781 cggcacatat cgttcagctc cggtggtacg ttgttgaact gaccttcgtt gtcgatgttt
    30841 aacacccagc tttggttatt cagacgtacg cgtacccggc aatcttccac aaggaagcct
    30901 gggttgctgc tgttatcaaa gtcttgcgac aggctgaaga cggtgatttt gtctttgtac
    30961 ggtttaccgt tccacgggca gaattgggcg cagttgccac attcgttaca gtaagcgtcg
    31021 aggtgcagcg tctggaaacg gttctggaag cctgggaccg caatggatac gttggcgcgg
    31081 ttcgggcaga catccacaca cttgctgcaa acgtagttac attcaaggca gcgtgcggct
    31141 tcctgggcga caaacgcgtc acgatcgtcg ctatccacca gggtgacaga gatatcgcct
    31201 ttgcgttgat agatttccgc cggattaacg ttgttccagt atttatcgtt ctggtgggaa
    31261 cggatatttt cccgactaag gatggcatcg gtcgcccgac gcgcggttcc gacagcagcg
    31321 acaatggagg atggtccgcg ctgtacgtcg ccgatcataa agacgtcagt cagacgagtt
    31381 tcgccattat ggtcgacgtc tggccagccg tttttgtcca gcggcacgcc catcgcattc
    31441 agggcttcag tatcctgctg ttcaccaatg gcggtgatca ggctgtctac atgcagtgtt
    31501 actgtttcgt tggtttcaac cggacggcga cgacctttct catcaggttc gccaagcgac
    31561 ataacgcgca aggttaaggt gccatcagca tcgaaacgtt ccggattatt cagaaaacgg
    31621 aactccacgc cgtcgtgcaa cgcttcttca tactcttcgc gccatgctgg catctcttgc
    31681 agtgaacgac ggtaaacgac cgttgctttt tctacgcctg gaacgcgtaa cgccgcacga
    31741 gcgcagtcca ttgcggtgtt acccgccccg acaacgacca catgtttgcc cagcttgagc
    31801 tctgtgccct tgttgtattc acgcaggaag gggagtgatt tccagacatt ttggttgtcg
    31861 cccgccagtt tcacaccgct atttttatca gtgccggtgg caatcagaac atagtggaag
    31921 ccctgatttt ttaactgttc aacggtcaga tcgggtgagc agccatactc aaatttcacg
    31981 ccgtgagcgg caacaaaatc gatatcgtgc tgaattaact ctggaggaat acggaactga
    32041 ggaatgatat ttttcaccac gccgcccgcg ttggcttcgc gttcaaacag cgtaaccgga
    32101 tggcccgctc tggcaaggaa gtaacctgcg gccagacccg ccggacctgc accaatcacg
    32161 gcaaccggat ggcgtgaacc agaaccggct ggtttgtgcc agcgttgctt atattcatcc
    32221 caaccttttt ccagcgcgac ttttttcagt tcgcggatat tcagcgcact gtcgtaatcc
    32281 aggcgggtac agttgtattg gcactggtga tcgcaaatat gaccggtaat ggcgggcagg
    32341 gcgttgcgtt gatagatgag ttccagcgcg tcggcatagc ggtgttcgcc aagcagacgg
    32401 atgtattccg gaatatcttg cttgatagcg caggcagtaa cacagggggc aacgtagcaa
    32461 tcggtcagcg gcaggtcttc agccacttca atacgctctt ctggcttcca gtgtttctgg
    32521 gtgtattcca tggttaacgc gtctgctgcc agtctgttca gtcgttcgac gtcaacgtgg
    32581 tcaagtcccc aggcgtcgga gccttccagc tcgcgcatgc aggcacttaa gcgcagatag
    32641 ccgccaggtt tcagcaggtc ggttgccata gtaatagggc gaataccagt atcaaaaata
    32701 tcgcggatag tcagctgact ggcaccaccg gaataagaaa tgggcagttt gccgtcaaag
    32761 gcgcgagaga gaactgctgc aacgttgatg gagagcggga acagcgcacg gcctgacata
    32821 tacatctctt caccgggcag tgcgcctttg ttattaatgg tgccgagagt gttagtcagt
    32881 tttacgccaa agccgagtga tttttctttt gccagtgcca tcaggcgttc cagcatttcc
    32941 agtgcttgcg tcagcttgag gtcgtgatca aatgactctt cttttaagcc gatgtaaccg
    33001 aaaccgcaga catcgaggat ctcacgaaca cgcgcgtacc ccagtaaggt cgggttgagt
    33061 ttcacaaagg tgttgagccc tttttcttcc agcatgtagc ggcaaatggc ttcgatttca
    33121 tgcggaggac agccgtgcat ggtggagagg gtaacccctt gtaccatgct ggtggggatg
    33181 cgagcgggta aggcttgcaa gctttcgcgt ttttcctgta agccgtggcg ggctaaaaat
    33241 gcgtcatcct ggagtagctt attaagtgca tcgcggtatt gagcgaattt cggatggtca
    33301 gatgcgtcca tcatattgtc gatgaactgc tgcatcggcg gttgcttaat accttcgagg
    33361 ttgtaaccga cgctcatatt aaagatgaac gatttacagg aatcagaagg ctggaacatc
    33421 gcttcgagaa ggtgcagggc aaaccaggct ttgaggtatt catcccaggc tttaagcagg
    33481 gtaaattcgg tagaccattc ggtgttaaag cactcgtctt cggcatcgat acagggcttt
    33541 tccagctcca ggcggtcaag aatttggacg gtttttagtt cgatgaagcg tccgccagtc
    33601 agccaggaag tgacaatatt ttgcgcgagc tgcgtgtgcg ggccagcggc agggccgacg
    33661 ggagtggcac aggtttcacc gaagacgcta acagttttac cttttacggg tgagtaaaac
    33721 tgttgctcgg gaataccaaa gattgagcgt tgttgttggt attcatcaaa tatgcgcgtc
    33781 aaaagttcct caaacggaat gggacgcata atatccccca taaccctctc cttagcttat
    33841 acagatatca ttgagtgacc ggtgttgaga aatccggtgg tttaggggga tagagcaaca
    33901 atcgcaccac tcttgccagg tttggcggga aatataaaaa aatgtgaggc ggctcgaaac
    33961 ttaatggctt tttgtgaatg aactcacagg tgccaaatga gaattaaaat cactcctgaa
    34021 aattattatt gccagcccat ttatcacttt ttctcttttc tcaaaatcag tgattcctta
    34081 tcattatgat aaagagtgct ggagggatta aatatttgaa cgcaatcgtt taactacgtt
    34141 ttctaaattt cagtatacct tttcttcgct gtaataaagt cgtcaggagt atcaatatct
    34201 aaaattattt ctgcattttc tatttcgacg gaataatgtt ctcccataag gagtgcctga
    34261 cgcatattgg tgacattggg tcgctggatt gcctgcatca ggcatggttt tgacactaaa
    34321 atcggatggc cggggatgcc attatggagt ggcagtattg cgccatcatt tcgtaacgac
    34381 cagatttttc taaaaatatc gatggtgagg gttggcatat ctccgtgggt gagaaaacaa
    34441 tgttctgttt gcaccgcggg tactgcggcc tttactgatg tcagtaaacc ctgcgcataa
    34501 tctgggttgt ggataatagt gatattgctc tggttcgcat aacgttcgtg cagttcatta
    34561 ccacgatagc cggtgactaa aataattcgg ctacaaaact gcaacgcatt tttgatactt
    34621 gtatcaagaa ttgttccctg ttgccagggt aacataattt tccattgccc cattcttgat
    34681 gataatcctg cggcggtaat tatacagtcg atggctgaca tagtgattcc attatttaca
    34741 ggagctaata acgcctgata attgcgttac ttcctgatgg attaagttga gggtaattta
    34801 tcccagagag gtccataaag acgcgtgaaa agtataattg acccatcggc attattcatt
    34861 gatttaggcg cgcagaaacg tcccactgtg atttctgttg ttggtgctgg ggggaaaacc
    34921 agcttgcttt tttggctggc agaactgttc caggcgagcg gcaggcgtgt attaatcact
    34981 acgacgacac atatgtttat gccaacatct cactggcccg tggttttctg tcgtgatccc
    35041 gccatgcttc ctcatgcgtc ttttatatcc cccatttcat tttgttttca cagctggaaa
    35101 gcgaaccagg gaaaagtgca gggatttacg ccagaagcga ttgatgcact ggtacaacga
    35161 tcagagtgtg acgtaattct cattgaggca gatggctcgc gtggaatgcc gttaaaagcg
    35221 cctgatgagc acgaaccttg catacctaaa agcagttgct gcgtgattgc tgtgatggga
    35281 ggacatattt tgggtgcgaa agtgagcaca gaaaatgtcc atcgctggtc gcagtttgct
    35341 gatattaccg ggttaacacc tgatgcaacc ttgcaactga gcgatctcgt tgcgctggtt
    35401 cgccaccctc agggggcgtt taaaaacgta ccgcaaggtt gtcggcgagt ctggttcatt
    35461 aaccgttttt ctcaatgtga gaatgcgatt gcgcaaagcg agctccttca accgctgcaa
    35521 caacacgacg tagaggcaat ctggctgggc gatatacaag aacatcctgc aatcgcgcgc
    35581 agatttgtga attagcggcc cgggtattcc gggttactga aaggatatcc attgaggttg
    35641 gtatgaatat tttcacagag gctgcaaaac tcgaagagca aaattgtccg tttgcgatgg
    35701 cgcaaattgt tgatagccga ggctcaaccc cccgccattc tgcacaaatg ttagtgcgcg
    35761 ccgatggctc tatcgtcggt acaattggtg gcggaatggt tgagcggaag gtgattgaag
    35821 agtcgcttca ggcattgcag gaacgtaagc cgcgattatt ccatggacgt atggctcgta
    35881 acggtgcgga tgctgtcggg tcagattgtg gaggtgcaat gtcagtattt atcagcgtcc
    35941 atggtatgcg tccacgtctg gtgttgattg gcgcggggca tgtcaaccgg gcgatagccc
    36001 agagtgcggc gctattagga tttgatatcg ccgttgccga tatttatcgc gaaagcctca
    36061 atcctgaact attcccacca tcaaccacgc ttctccatgc tgagtcgttt ggtgcggcag
    36121 tggaagcact ggatattcgc cctgataatt ttgtcctgat tgccacgaat aatcaggatc
    36181 gtgaagccct cgacaaactc attgaacaac ccattgcatg gctggggttg ctggcaagtc
    36241 gccgcaaggt tcagcttttc ctgcgccaat tgcgtgagaa aggcgtggct gaagaacata
    36301 ttgcccgttt acatgcgccc gttggttaca acataggtgc ggaaacgccg caggagatcg
    36361 ccatcagcgt gctggcagaa atattacagg tgaaaaataa cgcgccgggt gggctgatga
    36421 tgaagccttc tcatccttcc ggacaccagc tggtggtgat tcgtggtgcg ggggatatcg
    36481 ccagtggtgt agcgctacgt ctgtatcatg cgggttttaa agtgatcatg ctggaagtgg
    36541 aaaaaccgac agtgattcgt tgtaccgtgg catttgccca ggccgtgttc gatggcgaaa
    36601 tgacggtcga aggcgtcacc gcccgcctgg caaccagctc tgcggaggcg atgaaactta
    36661 ccgaatgcgg attcatccct gtgatagtag atcccgcctg ttcattgctt gatgaactga
    36721 aaccgctttg cgtggtggac gctattctgg cgaaacagaa tttggggacg cgggcagata
    36781 tggcaccagt aacaatcgcg cttgggccgg gctttactgc agggaaggat tgtcatgcgg
    36841 taattgaaac aaatcgcggg cactggctcg gtcaggtgat ttactctggt tgtgcgcagg
    36901 agaataccgg tgttcctggc aatattatgg ggcataccac ccgacgggtg atccgtgctc
    36961 ctgctgcagg cattatgcga tccaacgtga aattaggcga tctggtgaaa gagggcgatg
    37021 tgattgcctg gattggtgag catgaaatta aagcaccgtt gacggggatg gtgcgtggct
    37081 tgttgaacga cggactggca gtggtcggtg gttttaaaat tggtgatatc gatcctcgtg
    37141 gtgaaacggc tgatttcacc agcgtttctg ataaagcccg ggcgattggc ggcggcgtac
    37201 ttgaggcgtt aatgatgttg atgcatcagg gcgttaaagc gacgaaagaa gtgctggaag
    37261 tggcttaaat gaaaatggcc ggatggtatt acccacccgg cctttaaatt actgacaaat
    37321 caccgttccg gttttgcctt caatcccttc tttcgctttg ctcaatacgg taatcagtgc
    37381 ttcacgacct gcgcgagagc gagcaaacga ggcggcagct tccactttag gcagcataga
    37441 acctttcgca aaatgacctt cttcgataaa gcgttcagca tcacttaatg acaggcgatc
    37501 gagccactgt tcattctctt taccgaagtt aatggccact ttttctaccg cagtcagaat
    37561 gatcagcata tcggcatcaa tcatttctgc taaacgggcg ctggcccagt ctttatcgat
    37621 aaccgcactg gcaccgcgca gatggttccc ttcacgaata acgggaatgc caccaccgcc
    37681 aacggtgatc actacctggc ctgcttctac cagcgctttt accgtctctt tttcaataat
    37741 atcaaccggt ttcggcgagg caacaacacg gcgatagccg cgaccagcat cttctttcag
    37801 agtgtaaccc tgttttgtca gttgctctgc ttcctgctcg gtaaagaacg aaccgatcgg
    37861 cttggtcggg ttgaggaatg ccgggtcgtt agcatcgact tcgacctggg taaccagcgt
    37921 tgctacaggt ttattaatgc cacgagaaag cagttcttct cttaatgcgt tttgtagatc
    37981 gtaaccaata taaccctggc ttaaggcaac acagacagac atcggcagca ttggcgagtg
    38041 cgcttcagtt tttgcggcag cttcaaaggc ctggttaatc atgcccacct gtggcccgtt
    38101 accgtgagtg acgataactt catgtccctg ggcaattaaa tcaacaattg cctgagaagt
    38161 aatttttacc gctttcattt gtccggccag gtcatcgccc agcgcattcc cgcccagggc
    38221 gagaacaatt ttcttactca tttttcctca ctcatcattc ttgattgtgt atagatccct
    38281 ggcagtggtt agagcacggg agggacaaac ggtttgcgtc gcaggaaacg acctcgcccg
    38341 gctttgcctg taaaggtgcc gtcacagaaa atcgtttcac cacgggataa agttctgaca
    38401 atcgcgccct gacaggtaaa accctcccat ggcgagtagt cggcgttgtc gtggagatgg
    38461 cgatgctgaa tttgttgact ctgacgtggg tcgataatca ccacgtcgcc atcggaaccg
    38521 ggcgctaata atcctttttg cggccacagg ccaaacagcc tggcgggcat cgcgctggtt
    38581 aattcaacaa aacgttccgg tgttatacgt cccgtcataa cgccactgga aaacagtaac
    38641 tgcatgcggt tttccacacc gggtaagcca tttgggcagc gactgaaatc gcctttagaa
    38701 atttgcaggc gttgagccat cgagaatgtg cagtgatcgg tcgctaccac gtcaatcgca
    38761 ccatcgctga tgccacacca cagtttgtcc tgctcgcgta cgttacgcag cggtgggcta
    38821 agaatgaact tcatgccatc ttctgtatcg taactgcgtt cgtccaacag gagatattgt
    38881 gggcaggttt caacccagac tggctggtga ttcgcacggg caagacgcag ataatccaga
    38941 cctaagccgt tagacaggtg cacgatatag agcggggcgt taccggcaat ttgtgccagg
    39001 ttaatcatgc gggcgatggc ttccgcttcg cattccagag gtcgactcaa ggcatgataa
    39061 cgcggcgcgg ttaaccctgc ggcgataaac tccgcccgct tgctggcgat agctgcgtca
    39121 ttttccgggt gcacggtggt cagcgcgccg gattcatgca agcggcgtaa tgcctgcaac
    39181 acttcatcat cgttgagttt atattgatag gttaaataga gtttaaaact gctcagtcct
    39241 tcctcaacca tcatcggaat ttcgtcgagg atagcgtgat tgatatgttg aatgacaccg
    39301 tgaaagctgt agtcgatgac tgctttgtgg gcggcataac cgcgataaac ttccagttga
    39361 tggcgtaacc ggcagccatt cgggccaaat cccatatggt caataatggt tgttgtaccg
    39421 ccacacgcag ctgcgcgggt accggtaaaa aaatcatcac aactgcgcgc gatgccgaca
    39481 tcaatattga aatgcgtatg gacatccacg ccgccaggga aaacgtaaca gccagtggca
    39541 tcaatttctt catacgggac ctgcggcgaa atattgctgc ccaattggcg aacaatcccg
    39601 ctctcaatca gcaaatcttg tttggcttgt ccatctgcgt taacgacagt gccgtttttg
    39661 atcaatacgc gcatagcaaa ctccaaaaac cctccggcga accggagggg aataagtgaa
    39721 gagtaattat tcggttgcca gccagcttaa cgggattgca gcgtacatcg cggcacaggt
    39781 caccaggtga gatttccagg ttttttcgtt aggtgcatgc gcttcaggtt ctttacccgg
    39841 gccaaagccg atcaccggaa tgccgtgacg gcccatgata gatacgccgt tagttgagaa
    39901 ggtccactta tcaacaaccg gcgctttgcc aaacagacct tcgtaggcat tcaccagtgc
    39961 tttaacggtg aagtgatctt cttccacttt ccaggtcggg aagtagcatt cggttgggta
    40021 aaccaggcca gtccaggacg gacggtcgta gttgtacata gaaacaaccg cgttagcttt
    40081 ctgtactgca ggcagggcgc ggatttcgtc cagcgcgcct tcccaggttt cgccccaggt
    40141 cagacggcgg tcaatagaga cggcgcagct atctgctaca gcgcaacggc ttggggatgt
    40201 gaagaagatt tcagaaacgg tgagggtgcc tttgccgagg aattcgtcat aacccagacg
    40261 ttgggagagt tcttgtaatt cgccaagaat cggacccatt ttgaaaatgg cgttgtcacc
    40321 gcgttctggt gcagaaccgt ggcagctcac accctgaaca tcaatacgaa tttccatacg
    40381 accgcgctga ccacggtata cctggcagtc ggttggttcg gtactgacca caaattccgg
    40441 gcgaatgccg gattgttcaa taatgtactg ccagcacagg ccgtcgcagt cttcttcctg
    40501 cacagtaccg gtaaccagca gggtatattc atcttccaga ccgaggtctt taatgatttt
    40561 accggcataa accatagatg ccatgccgcc ttcctggtcg gaagtaccgc gaccaccgat
    40621 cagctcatca gtttccatgc cttcgtacgg atcgaagtcc cagtttttga tgttgccaat
    40681 gccgacggta tcgatatgag cgtccattgc caccagacgc gggccgtggc cgatataacc
    40741 gagaacgttg cccatcgggt cgatttcaac tttatcgaag ccgacttttt ccatctcttc
    40801 tttaatacga tgtactacgc gtttctcgtc gcagctttca ctgggaatag caaccatatc
    40861 gcgcaggaag cgagtcatgt ctgcctggta atcttttgct ttttcaagaa tcagtttgaa
    40921 tggaatattc ttagccatta tttttctcca gattttttac tccccgtttt gcagggagca
    40981 gttccaatag ggtgattaag gtgctacagc gtgtttgcct tcccagacaa cttcgcggta
    41041 gtgcttcacg tcggtgtcgc cttcagtgct gataaccagc accacggcat ctttgttcag
    41101 cgccagtttt tccatcaggc tttgacgttg cgggtgataa tgaaccgctg cgagaacgcc
    41161 caaaccgaca gcgccggatt caccggagat gatgcgcggg tcgttgccat acggattacc
    41221 cagcacgcgc atacctaatg cggcaacgct gtcctggcag gagatgaatt gggtggcgca
    41281 gttacgtagg atttcccagc ccagtgggtt aggttcgccg caggccaggc ccgccatgat
    41341 ggtggccata tcaccgccaa cgttgacgat gtcgcctttg acgccggagc gataaataca
    41401 gtcagctttg tcaggttcaa caataatgct gtgcagattt tgcgggctat agacgtcgac
    41461 cagataaccc agcacaccac cggccattgc accgacaccg gcttgcagca gaacgtgtgt
    41521 cggggtgacg cccatttcac gcatttgctc gacggcttca tccgccagtg ttgcgtagcc
    41581 ttgcatgatc caggttggga ttttggtgta accttcccat gccgtgtcct gtaccacttc
    41641 ccagccgtgc tgctgcgcgt gttgcatggt caggcgaacg gtatcgtcat agttcatatc
    41701 cgtgacgatg cactcggcac cgaggtttag aatggcgtca acgcgttcct gagcagaacc
    41761 tttcggcatg taaatcaccg cattctgtcc gagttgctgt gctgcccatg ccacaccgcg
    41821 cccgtggttg ccgtcggtgg tagtcgcgaa agtcattttt tcgccgatgg catttttcag
    41881 gtgctcaaat gacagcgttt cgatatcaag atgatatttt tcacacaata attgagcgat
    41941 ggcgtacgca ccgccaagca ttttgaaggc gttcagaccg aatcgttttg attcgtcttt
    42001 gacgagaatt ttctttacac caaacaggtt tgcgagatcg tccagcgcac aaagcggtgt
    42061 tgggtgataa ccagcaattt tctggtggaa ctggcgtgcc agttttgcct ggctttgcga
    42121 aaaaagcggt gatgtttcgc cgttgaaaaa tttgttatcg gcgatatcaa tcttcaatga
    42181 gaaaacggac atatcctatc cttttggcct ctggaaatag agggccgacc agctgatcgg
    42241 cccgaggggt tatttcacgc gttcttgcgc ttctttcagc agttgctcga gcagtgcacc
    42301 tggtttggcg tatttacggg acaggatcat cgcggcgatg atataaggct tccagctggc
    42361 ttctttgtag gtagcgatac ggtatttttc gaatacgcct tcagtcactt caccttcttt
    42421 gcaggatacg ccgctgatat cagctggcag gcagtgcatg tacagggctt cgccatcacg
    42481 ggtcagttcc atcatctctt cagtacaatg ccagtctttg tgttgcgcgt tctgtgccag
    42541 acactgtttt tccagtgctt ttaagccttc gtgatcgttc gcacgcagca attcagtacg
    42601 ctcttccatc actttgtaag gtgcccatga cttcggataa acgatgtctg cgtctttgaa
    42661 ggcttcttcc atgctggtga cctgacggaa gctaccaccg gaggctttag cattgttttt
    42721 agcaacttca acgacatccg ggatcaggtc gtagccttcc ggatgggcca gggtgacatc
    42781 cataccgaag cgggtcatca gaccgatgat gccttgtggt acagagagcg gtttgccata
    42841 gcttggggag taggcccagg tcatggcgat ttttttgcct ttcaggtttt ccagcgaacc
    42901 aaagtgttca cgcaaccatg ccaggtcagc catagactga gtcgggtggt caatatcgca
    42961 ttgcaggttc actaaagccg gacgctgtgg cagaacccct tgtttgtagc cgtcatcaag
    43021 tgccgcgcca acttcacgca tataggcgtt gcctgcgccc agatacatat cgtcgcgaat
    43081 accgatggca tcggcgcaga aggagatcat attggcggtt tcacgcacgg tttcgccgtg
    43141 agcgatttgt gatttgcctt cgtcgagatc ttgctgtgca aggccgagca ggttaagcgc
    43201 ggaagcataa gagaagcggg tacgggtgga gttgtcgcgg aatacggaaa tacctaatcc
    43261 actattaaag actttggttg agatgttttc agcacgcagt gcttttaatg ctgcggcaac
    43321 gtccagtact tgtttcagtt catctggcgt ctgttcccac gttaacaaaa aatctttctc
    43381 gtgaaggtga gaggtcagcg aattgatatc cttaatcagc tcattaacag ttttcatcat
    43441 aaagtcccgg tagtggaatg acaggcaaag tggtttattt cactgtgact tgccaacgtg
    43501 attggtgttt tcacagctat acctgggact aacaataagt gtgccagaat tatccacggg
    43561 gctgcaaaag actaatttga cggataatgt gtggggttga tcacaaattg atgccagctt
    43621 ctggttgttt gtaaaatacg aaaacgtttg gcttttttta tcgcgttatt tatttgctgc
    43681 taagtatcag aaataagata aagggacagg cgatctctct tcagtttgat aaac
//
LOCUS       DAZKJR010000023        41904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKJR010000023 DAZKJR010000000
VERSION     DAZKJR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.023"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            161..1060
                     /gene="lysO"
                     /locus_tag="ACHOWS_001299"
     CDS             161..1060
                     /gene="lysO"
                     /locus_tag="ACHOWS_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFQ0046913.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1204..2856
                     /gene="hcp"
                     /locus_tag="ACHOWS_001300"
                     /gene_synonym="priS"
     CDS             1204..2856
                     /gene="hcp"
                     /locus_tag="ACHOWS_001300"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFQ0046914.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2868..3836
                     /gene="hcr"
                     /locus_tag="ACHOWS_001301"
     CDS             2868..3836
                     /gene="hcr"
                     /locus_tag="ACHOWS_001301"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFQ0046915.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3969..5687
                     /gene="poxB"
                     /locus_tag="ACHOWS_001302"
     CDS             3969..5687
                     /gene="poxB"
                     /locus_tag="ACHOWS_001302"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFQ0046916.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5724..6725
                     /gene="ltaE"
                     /locus_tag="ACHOWS_001303"
     CDS             5724..6725
                     /gene="ltaE"
                     /locus_tag="ACHOWS_001303"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFQ0046917.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6736..8166
                     /gene="ybjT"
                     /locus_tag="ACHOWS_001304"
     CDS             6736..8166
                     /gene="ybjT"
                     /locus_tag="ACHOWS_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFQ0046918.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKA
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            8265..9278
                     /gene="ybjS"
                     /locus_tag="ACHOWS_001305"
     CDS             8265..9278
                     /gene="ybjS"
                     /locus_tag="ACHOWS_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0046919.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9275..10105)
                     /gene="amiD"
                     /locus_tag="ACHOWS_001306"
     CDS             complement(9275..10105)
                     /gene="amiD"
                     /locus_tag="ACHOWS_001306"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFQ0046920.1"
                     /translation="MRRVFWLVAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTLVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10102..10425)
                     /gene="ybjQ"
                     /locus_tag="ACHOWS_001307"
     CDS             complement(10102..10425)
                     /gene="ybjQ"
                     /locus_tag="ACHOWS_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0046921.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            10551..11066
                     /gene="ybjP"
                     /locus_tag="ACHOWS_001308"
     CDS             10551..11066
                     /gene="ybjP"
                     /locus_tag="ACHOWS_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0046922.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            11284..12012
                     /gene="artP"
                     /locus_tag="ACHOWS_001309"
     CDS             11284..12012
                     /gene="artP"
                     /locus_tag="ACHOWS_001309"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFQ0046923.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            12030..12761
                     /gene="artI"
                     /locus_tag="ACHOWS_001310"
     CDS             12030..12761
                     /gene="artI"
                     /locus_tag="ACHOWS_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFQ0046924.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            12768..13484
                     /gene="artQ"
                     /locus_tag="ACHOWS_001311"
     CDS             12768..13484
                     /gene="artQ"
                     /locus_tag="ACHOWS_001311"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFQ0046925.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTVLRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            13484..14152
                     /gene="artM"
                     /locus_tag="ACHOWS_001312"
     CDS             13484..14152
                     /gene="artM"
                     /locus_tag="ACHOWS_001312"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFQ0046926.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            14378..15109
                     /gene="artJ"
                     /locus_tag="ACHOWS_001313"
     CDS             14378..15109
                     /gene="artJ"
                     /locus_tag="ACHOWS_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFQ0046927.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(15138..16265)
                     /gene="rlmC"
                     /locus_tag="ACHOWS_001314"
     CDS             complement(15138..16265)
                     /gene="rlmC"
                     /locus_tag="ACHOWS_001314"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFQ0046928.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLSVKHMWDLFCGVGGFGLHCAMPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYCIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(16306..16794)
                     /gene="ybjO"
                     /locus_tag="ACHOWS_001315"
     CDS             complement(16306..16794)
                     /gene="ybjO"
                     /locus_tag="ACHOWS_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFQ0046929.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(16854..17699)
                     /gene="potI"
                     /locus_tag="ACHOWS_001316"
     CDS             complement(16854..17699)
                     /gene="potI"
                     /locus_tag="ACHOWS_001316"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFQ0046930.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDNAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(17696..18649)
                     /gene="potH"
                     /locus_tag="ACHOWS_001317"
     CDS             complement(17696..18649)
                     /gene="potH"
                     /locus_tag="ACHOWS_001317"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFQ0046931.1"
                     /translation="MSTLEPAAQPKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(18659..19792)
                     /gene="potG"
                     /locus_tag="ACHOWS_001318"
     CDS             complement(18659..19792)
                     /gene="potG"
                     /locus_tag="ACHOWS_001318"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFQ0046932.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(19887..20999)
                     /gene="potF"
                     /locus_tag="ACHOWS_001319"
     CDS             complement(19887..20999)
                     /gene="potF"
                     /locus_tag="ACHOWS_001319"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFQ0046933.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(21350..21826)
                     /gene="ybjN"
                     /locus_tag="ACHOWS_001320"
     CDS             complement(21350..21826)
                     /gene="ybjN"
                     /locus_tag="ACHOWS_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFQ0046934.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(21914..22816)
                     /gene="rimK"
                     /locus_tag="ACHOWS_001321"
     CDS             complement(21914..22816)
                     /gene="rimK"
                     /locus_tag="ACHOWS_001321"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFQ0046935.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(22877..23599)
                     /gene="nfsA"
                     /locus_tag="ACHOWS_001322"
     CDS             complement(22877..23599)
                     /gene="nfsA"
                     /locus_tag="ACHOWS_001322"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFQ0046936.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(23583..23870)
                     /gene="ybjC"
                     /locus_tag="ACHOWS_001323"
     CDS             complement(23583..23870)
                     /gene="ybjC"
                     /locus_tag="ACHOWS_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFQ0046937.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            24030..24287
                     /gene="grxA"
                     /locus_tag="ACHOWS_001324"
     CDS             24030..24287
                     /gene="grxA"
                     /locus_tag="ACHOWS_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFQ0046938.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(24317..24694)
                     /gene="ybjM"
                     /locus_tag="ACHOWS_001325"
     CDS             complement(24317..24694)
                     /gene="ybjM"
                     /locus_tag="ACHOWS_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFQ0046939.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            24964..26649
                     /gene="ybjL"
                     /locus_tag="ACHOWS_001326"
     CDS             24964..26649
                     /gene="ybjL"
                     /locus_tag="ACHOWS_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFQ0046940.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(26825..27361)
                     /gene="rcdA"
                     /locus_tag="ACHOWS_001327"
     CDS             complement(26825..27361)
                     /gene="rcdA"
                     /locus_tag="ACHOWS_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFQ0046941.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            27445..28653
                     /gene="ybjJ"
                     /locus_tag="ACHOWS_001328"
     CDS             27445..28653
                     /gene="ybjJ"
                     /locus_tag="ACHOWS_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0046942.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            28653..29468
                     /gene="ybjI"
                     /locus_tag="ACHOWS_001329"
     CDS             28653..29468
                     /gene="ybjI"
                     /locus_tag="ACHOWS_001329"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFQ0046943.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFSTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            29553..29837
                     /gene="ybjH"
                     /locus_tag="ACHOWS_001330"
     CDS             29553..29837
                     /gene="ybjH"
                     /locus_tag="ACHOWS_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFQ0046944.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(29878..31110)
                     /gene="mdfA"
                     /locus_tag="ACHOWS_001331"
     CDS             complement(29878..31110)
                     /gene="mdfA"
                     /locus_tag="ACHOWS_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFQ0046945.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHVWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            31395..31991
                     /gene="ybjG"
                     /locus_tag="ACHOWS_001332"
     CDS             31395..31991
                     /gene="ybjG"
                     /locus_tag="ACHOWS_001332"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0046946.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWSMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            32049..32807
                     /gene="deoR"
                     /locus_tag="ACHOWS_001333"
     CDS             32049..32807
                     /gene="deoR"
                     /locus_tag="ACHOWS_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFQ0046947.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(32854..34056)
                     /gene="dacC"
                     /locus_tag="ACHOWS_001334"
     CDS             complement(32854..34056)
                     /gene="dacC"
                     /locus_tag="ACHOWS_001334"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFQ0046948.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            34303..34929
                     /gene="gstB"
                     /locus_tag="ACHOWS_001335"
     CDS             34303..34929
                     /gene="gstB"
                     /locus_tag="ACHOWS_001335"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFQ0046949.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(34926..36041)
                     /gene="yliI"
                     /locus_tag="ACHOWS_001336"
     CDS             complement(34926..36041)
                     /gene="yliI"
                     /locus_tag="ACHOWS_001336"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFQ0046950.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESDARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(36152..36535)
                     /gene="bssR"
                     /locus_tag="ACHOWS_001337"
     CDS             complement(36152..36535)
                     /gene="bssR"
                     /locus_tag="ACHOWS_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFQ0046951.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            36748..38073
                     /gene="rimO"
                     /locus_tag="ACHOWS_001338"
     CDS             36748..38073
                     /gene="rimO"
                     /locus_tag="ACHOWS_001338"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFQ0046952.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            38299..38685
                     /locus_tag="ACHOWS_001339"
     CDS             38299..38685
                     /locus_tag="ACHOWS_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0046953.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVIQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            38675..39004
                     /locus_tag="ACHOWS_001340"
     CDS             38675..39004
                     /locus_tag="ACHOWS_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0046954.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(39074..40402)
                     /gene="dgcI"
                     /locus_tag="ACHOWS_001341"
     CDS             complement(39074..40402)
                     /gene="dgcI"
                     /locus_tag="ACHOWS_001341"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFQ0046955.1"
                     /translation="MSRINKFVLTVSLLIFIIISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQLPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(40410..41904)
                     /locus_tag="ACHOWS_001342"
                     /pseudo
     CDS             complement(40410..41904)
                     /locus_tag="ACHOWS_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT        10349 a        10862 c        10434 g        10259 t
ORIGIN      
        1 gcacatgaaa ggagcgcaac aatgctaaac actcatatag aattaaaaat ctccctctga
       61 tgcataacat ttccttttaa ttgcaacttt actcgtcctt ccgctttcag tagtgtgtca
      121 tcgcgcattt cgacttcatc tttgcaagga ctgggttttc atgttttctg ggctgttaat
      181 cattctggtt cccctgattg tgggttacct cattccgctt cgccaaaaag ctgcattaag
      241 agttattaat cagctattaa gctggatggt ttaccttatt ctctttttta tgggtatcag
      301 tctggcgttt ctcgataacc tcgccagtaa cctgttggcg attctgcatt attctgccgt
      361 cagtattacc gttattttac tgtgtaatat tgccgccctg atgtggctgg agcgcggcct
      421 gccatggcgc aaccaccatc atcaagaaaa actcccgtcg cgtattgcga tggcgctgga
      481 gtcgctaaaa ctgtgcggcg tagtagtgat tggttttgcc attggtctga gtggactggc
      541 tttcttacaa cacgcgaccg aagccagtga atacacctta attctgctac ttttcctcgt
      601 cggtattcag ttgcgcaata atggcatgac cttaaagcag attgtcctta atcgccgggg
      661 aatgattgtc gccgtggtgg tggttgccag ttcattaatt ggtggtttaa ttaacgcctt
      721 tattcttgat cttcccatca ataccgcgct ggcaatggcc tccggtttcg gctggtattc
      781 tctttccggt attttattga ccgaatcttt tggtccggta atcgggagcg cggcgttttt
      841 taatgatctg gcccgtgagc tgattgctat tatgttaatc cctgggctga ttcgccgtag
      901 ccgctctact gcactgggct tatgcggtgc gacgtcgatg gatttcaccc tgcccgttct
      961 tcaacgtaca ggcgggctgg atatggtccc ggcggcgatt gttcacggtt ttattcttag
     1021 tctgttagtg ccgattctca tcgccttttt ctctgcataa tacctctctg gcggtagatc
     1081 tctgccgcca aaattgcgct aaatcaatct cccttaaagt tgcattaaaa atctctttta
     1141 tccccgcgtt aagcgtctta accttaaaca tgtatattaa atataacttt aaaaggtgtg
     1201 atcatgtttt gtgtgcaatg tgaacaaact atccgtactc cggcaggaaa cggctgctca
     1261 tacgcgcagg ggatgtgtgg taaaacggca gaaacctctg accttcagga tttactcatc
     1321 gcggcgctgc aagggctttc ggcctgggca gtgaaagcac gtgaatacgg cattatcaac
     1381 cacgatgtag acagctttgc gccacgtgcc tttttctcaa ccctgaccaa cgttaacttc
     1441 gattctccgc gtattgtcgg ctacgctcgt gaagcgattg ccctgcgcga ggcgctgaaa
     1501 gcacaatgcc tggctgtaga tgccaacgcc cgcgtcgata acccgatggc tgacctgcaa
     1561 ctggtgagcg acgatctcgg cgagctgcaa cgtcaggcag cagaatttac ccctaacaaa
     1621 gataaagcgg cgattggcga aaacattctc ggcctgcgtc tgctgtgcct gtatggcctg
     1681 aaaggtgcgg cggcctatat ggaacacgcg catgttctcg gtcaatacga caacgatatt
     1741 tatgcccagt accataaaat catggcgtgg ctggggacct ggcctgccga tatgaacgcg
     1801 cttcttgagt gttcaatgga aatcggccag atgaacttca aagtgatgag cattctggac
     1861 gcaggcgaaa ccggtaaata cggtcacccg acgccaactc aggtcaacgt caaagcgacg
     1921 gcgggtaaat gcattctgat ttccggtcac gatctcaaag atctctacaa cctgctggaa
     1981 cagaccgaag gcacgggcgt taatgtttac acccacggcg aaatgctgcc tgcgcatggc
     2041 tacccggagt tgcgtaaatt caagcatctg gtcggtaact acggcagcgg ctggcagaat
     2101 cagcaagtgg agttcgctcg tttcccaggc cccatcgtga tgacctcgaa ctgcatcatc
     2161 gacccaaccg taggcgctta tgacgatcgt atctggaccc gcagcatcgt tggctggccg
     2221 ggtgtgcgtc atctggatgg cgaagatttc tctgcagtca ttgcccaggc gcaacagatg
     2281 gcgggcttcc cgtacagcga aattccgcac ctgatcaccg tgggctttgg tcgccagaca
     2341 ctgcttggcg cagccgatac gctgattgat ctggtgagcc gtgaaaaact gcgtcatatc
     2401 ttcctgcttg gcggctgtga cggcgcacgc ggcgagcgtc actatttcac cgatttcgcc
     2461 accagcgtgc cggatgactg cctgatcctg accctcgctt gcggtaaata tcgctttaat
     2521 aaacttgagt ttggcgatat cgaaggtctg ccgcgtctgg tagatgctgg tcaatgtaac
     2581 gatgcttact cagcaattat tctggcggta acgctggcag agaaactggg ctgcggagtc
     2641 aacgatctgc cgctgtcgct ggtgctctcc tggtttgaac agaaagcgat tgtcattctg
     2701 ctgacgctgc tttctctggg tgtgaaaaat atcgtcaccg gcccgactgc acctggtttc
     2761 ctgacaccag acctgctggc ggtgctgaac gagaaattcg gcctgcgttc tatcaccact
     2821 gttgaagaag acatgaagca actgttgagc gcgtaaggag tttgtcgatg acgatgccaa
     2881 cgaatcaatg cccgtggcgg atgcaggttc atcacattac gcaagaaacg ccggatgtgt
     2941 ggacgatttc cctgatttgc cacgattact acccatatcg cgccgggcaa tatgcactgg
     3001 tcagcgtgcg taactcagcg gaaacgctgc gtgcttacac catttcctcc acgccaggcg
     3061 tgagtgaata catcaccctg actgtgcggc ggattgatga cggtgtcggc tcccagtggc
     3121 tgacgcgcga tgtaaaacgc ggtgattatc tctggctttc ggacgcgatg ggggaattta
     3181 cctgcgatga taaagcagaa gataaattcc tgttgctggc ggcaggctgc ggcgttacgc
     3241 cgattatgtc gatgcgtcgc tggctggcga agaaccgtcc acaggccgat gtgcaggtga
     3301 tctacaacgt gcgtacgccg caggatgtga ttttcgccga tgagtggcgt aactatccgg
     3361 taacgctggt ggcggaaaat aacgttaccg aaggctttat cgctggtcgt ctcactcgcg
     3421 aactgctggc aggtgtccct gatttagcct cacgtaccgt gatgacctgt ggccctgctc
     3481 catatatgga ttgggtagag caggaagtga aagcgctcgg cgtgacgcgt ttctttaaag
     3541 agaaattctt caccccagta gcggaagcgg cgaccagcgg tctgaaattc accaaactgc
     3601 aaccggcacg agaattttac gccccggttg gcaccacgct actggaggcg ctggaaagca
     3661 ataacgttcc ggttgtcgcc gcctgccgcg caggtgtttg cggctgctgt aagacgaaag
     3721 tggtttccgg tgaatatacg gtgagcagca caatgacgct gaccgacgcc gaaatcgctg
     3781 aaggttatgt actggcctgc tcctgccatc cgcaggggga tttggttctc gcataatcgc
     3841 ctgatgcccg atgatattcc tttcatcggg ctatttaacc gttagtgcct cctttttctc
     3901 ccatcccttc cccctccgtc agatgaacta aacttgttac cgttatcaca ttcaggagat
     3961 ggagaaccat gaaacaaacg gttgcagctt atatcgccaa aacactcgaa tcggcaggcg
     4021 tgaaacgcat ctggggagtc acgggcgact ctctgaacgg tcttagtgac agtcttaatc
     4081 gcatgggcac catcgagtgg atgtccaccc ggcatgaaga agtggcggca tttgccgctg
     4141 gcgctgaagc acaacttagc ggagaactgg cggtctgtgc cggatcgtgc ggtcccggca
     4201 acctgcactt aatcaacggc ctgttcgatt gccaccgcaa tcacgttccg gtactggcga
     4261 ttgccgctca tattccctcc agcgaaattg gcagcggcta tttccaggaa acccacccac
     4321 aagagctatt ccgcgaatgt agtcactatt gcgagctggt ttccagccca gagcagatcc
     4381 cacaagtgct ggcaattgct atgcgcaaag cggtgcttaa ccgtggcgtt tccgttgttg
     4441 tgttaccggg cgacgtggcg ttaaaacctg cgccagaagg ggcaactacc cactggtatc
     4501 atgcgccaca gccggtagta acaccggaag aagaagagtt acgcaaactg gcgcagctgc
     4561 tgcgttattc cagcaatatc gccctgatgt gtggcagcgg ctgtgcgggg gcgcataaag
     4621 agttagttga gtttgccggg aaaattaaag cgcctatagt ccatgccctg cgcggtaaag
     4681 aacatgtcga atacgataat ccatatgatg ttggaatgac gggattaatc ggcttttcgt
     4741 caggtttcca taccatgatg aacgccgaca cgttagtgct gctcggtact cagtttccct
     4801 accgtgcgtt ctacccgacc gatgccaaaa ttattcagat tgatatcaac ccagccagca
     4861 tcggcgctca cagcaaggtg gatatggcac tggtcggcga tatcaagtcg actctgcgtg
     4921 cattgctgcc attggtggaa gaaaaagccg atcgcaagtt tctggataaa gcgctggaag
     4981 attaccgcga cgcccgcaaa gggctggacg atttagctaa accgagcgag aaagccattc
     5041 acccgcaata tctggcgcag caaattagtc attttgccgc cgatgacgct attttcacct
     5101 gtgacgttgg tacgccaacg gtgtgggcgg cacgttatct aaaaatgaac ggcaagcgtc
     5161 gcctgttagg ttcgtttaac cacggttcga tggctaacgc catgccgcag gcgctgggtg
     5221 cgcaggcaac cgagccggaa cgtcaggtgg tcgccatgtg cggcgatggc ggttttagca
     5281 tgttgatggg cgatttcctc tcagtagtgc agatgaaact gccggtgaaa attatcgtct
     5341 ttaataacag cgtgctgggc tttgtggcga tggagatgaa agccggaggc tacctgacag
     5401 acggtactga gctgcacgac accaactttg cccgcattgc cgaagcgtgc ggcattacgg
     5461 gtattcgtgt agaaaaagcc tctgaagtcg atgaagccct gcaacgcgcc ttctccatcg
     5521 acggtccggt gttggtggat gtggtggtcg ccaaagaaga gttagccatt ccaccgcaga
     5581 tcaaactcga acaggccaaa ggtttcagcc tgtatatgct gcgcgcaatt atcagcggac
     5641 gcggcgatga agtgatcgag ctggcgaaaa cgaactggct aaggtaaaaa gggtggcatt
     5701 tcccgtcata ataaggacat gccatgattg atttacgcag tgataccgtt acccgaccga
     5761 gccgcgccat gctcgaagcg atgatggccg ccccggttgg ggacgacgtt tacggagacg
     5821 accctaccgt taatgctctg caggactatg cagcagagct ttccggtaaa gaagccgcca
     5881 tttttctgcc taccggcact caggccaacc tggtcgctct gctcagtcac tgcgaacgcg
     5941 gcgaagagta tatcgtcggt caggccgcgc ataactatct gtttgaagcc ggtggcgcag
     6001 cagtgctggg cagtattcag ccgcaaccca tcgacgcggc tgccgacggc acgctacctc
     6061 tggataaagt ggcgatgaag atcaaacccg acgatatcca tttcgcccgc accaaattac
     6121 tcagtctgga aaacacccac aacggcaaag tgttgccgcg ggaatacctg aaagaagcat
     6181 gggaatttac tcgcgagcgc aatctggcgc tgcatgttga cggtgcgcgc atctttaatg
     6241 ccgtggtggc ttacggctgc gaactgaaag agatcacgca atattgtgat tcgttcacca
     6301 tttgcctgtc gaaaggtctt gggacgccgg tcggttcatt actcgtcggt aatcgtgatt
     6361 acattaaacg tgccattcgc tggcggaaaa tgacaggtgg cgggatgcgt cagtccggca
     6421 ttctggctgc cgccgggatg tatgccctga aaaataacgt cgcacgtttg caggaagacc
     6481 acgataacgc cgcctggatg gcggagcagt tgcgtgaagc aggcgcggat gtgatgcgtc
     6541 aggacaccaa tatgctgttt gttcgcgtcg gggaagaaaa tgctgccgcg ttaggcgaat
     6601 acatgaaagc gagaaacgtg ctgattaacg cctcgccgat tgtccgcctg gtgacccatc
     6661 ttgacgtctc gcgtgaacaa ctggcggaag ttgccgccca ctggcgtgcg ttcctggcgc
     6721 gttaaggaga gagacgtgcc gcaacgcatt ttagttctcg gtgccagtgg ctacattggt
     6781 cagcatctgg tgcgaactct cagccagcaa gggcatcaga tcctggcggc ggcacgtcat
     6841 gtcgacaggc ttgcaaagct gcaactggca aacgtcagtt gccataaagt cgatctcagc
     6901 tggccggata accttccggc cctgttgcag gatatcgata cggtctattt tctggtgcac
     6961 agcatgggcg aaggcggcga ttttatcgct caggagcgcc aggtggctct caacgtccgc
     7021 gatgcgctac gcgaagtccc tgttaagcaa ttaatcttcc tcagttcgtt gcaggccccg
     7081 gcacatgagc agtctgacca tctgcgcgcc cgtcaggcta cggcggatat tcttcgtgaa
     7141 gcgggtgtcc cggtgaccga actacgtgcc ggaataatcg ttggtgcagg ttcagcggcg
     7201 ttcgaagtca tgcgcgatat ggtctacaac ctgccggtgt taacgccgcc acgctgggtt
     7261 cgttcacgca ccacgcccat cgcgctggaa aacttgctgc actatctggt ggcgctgtta
     7321 gatcatccgg ccagcgaaca tcgcatcttc gaagccgccg gaccagaagt gctcagttat
     7381 cagcaacagt ttgaacattt tatggcggtg agcggtaagc gccgctggtt gatccccatc
     7441 cccctcccca cccgctggat ttcggtgtgg tttctcaatg tgattacttc cgtaccgccc
     7501 accaccgcca gggcgttgat tcaggggctg aaacatgatc tgctggcgga tgacaccgcg
     7561 ctacgtgcac tcatcccaca acggctgatc gctttcgatg acgcggtacg tcgcaccctg
     7621 aaagaagaag aaaagctggt caactccagc gactggggct acgacgctca ggcctttgcc
     7681 cgctggcgac cggagtacgg ttatttcgcc aaacaggcgg ggtttaccgt taaagcgtcc
     7741 gccagccttg cggctttatg gcaggtggtg aatcaaatcg gcggtaaaga gcgttatttc
     7801 tttggcaata ttttgtggca gacacgggcg ttgatggacc gcgcgatcgg tcataagctg
     7861 gcgaaaggcc gcccggagcg tgaatatttg cagaccggcg atgcggtgga tagctggaaa
     7921 gtgattgtcg ttgaaccgga aaaacaactt acgctgttat ttggcatgaa agcgccgggg
     7981 ctgggacgac tgtgttttac cctggaagat aaaggtgact atcgtactat cgatgttcgc
     8041 gcattctggc atccgcacgg tatgccgggg ctgttttact ggttattgat gatccccgcg
     8101 catctgttta tttttcgcgg aatggcaaaa cgaatcgcca gactggcaga acaaagcaca
     8161 gattaatcga caaaacctgc gttattcttt catgtttccc attgcatcat gggggaaatc
     8221 acggaagaat gcgcacgtta ttttctcaac aacagtggat tgatatgaag gtactggtta
     8281 ccggcgccac cagcggctta ggtcgaaacg cggtagagtt tttatgccag aaaggcatca
     8341 gcgtgcgagc gaccggtcgc aacgaggcaa tgggtaaatt gctggaaaaa atgggcgcag
     8401 agtttgttcc ggcggatctg accgagctgg tctcatcaca agctaaagtg atgctcgcgg
     8461 gcattgatac gctgtggcac tgctccagct ttacctcgcc ctgggggaca caacaggctt
     8521 tcgatctggc taacgttcgc gccactcgcc gcctgggtga atgggctgtc gcctggggtg
     8581 tgcgtaactt tattcatatc tcttcaccct ccctgtactt cgattatcac caccatcgcg
     8641 atattaaaga agattttcgc cctcaccgct tcgccaacga gtttgcccgt agcaaagcag
     8701 ccagcgaaga ggtgatcaat atgctttcgc aggcgaatcc acaaacgcgc tttactattc
     8761 tgcgcccaca aagtctgttc ggaccgcacg ataaagtctt tattccccgt ctggcgcata
     8821 tgatgcacca ctacggcagc attctgttac cgcatggcgg cagtgcgctg gtcgatatga
     8881 cctactatga aaatgccgtg cacgccatgt ggctggcaag ccaggaagcc tgcgataagc
     8941 taccttccgg gcgtgtgtac aacatcacca acggcgagca tcgcacactg cgcagcatcg
     9001 tgcagaagct gatcgacgag ttgaatattg actgtcgtat tcgttccgtc ccctacccga
     9061 tgctggatat gatcgcccgc agcatggagc gtttaggccg caagtcagca aaagagccgc
     9121 cgctgaccca ctacggcgtc tccaagttga attttgactt tacgctggat attacgcgcg
     9181 cgcaggaaga gttaggttat cagccggtca tcaccctgga tgaaggtatc gagaaaactg
     9241 ccgcctggct acgcgaccac ggaaaactgc cgcgctaatc ctgcccgtat ttctccagca
     9301 acgcttcggc aatcgcctga gtttctgcat ccgcttcgcc gttgtataac gtcgggcgga
     9361 aatgcatctg gaacgccata atcacgcgcc gctgctcgcg cggtgtcata tcaggtttaa
     9421 cgtcataacc gtagcgcgcc aaaagctcca gtaatgatgc ggtatctacc agtgtgtgcg
     9481 gcgcgcgccc ggcaaggtaa aagttcaccc gctgcgcatc cggccaggca ccaatcccct
     9541 gctgcgccag ttgttgccag gggaataacg gccccggatc gtctttgcgc tgcggcgcaa
     9601 tatccgcatg cgccactacg ttttccggct tgatgtgata acgggcaata atatctttcg
     9661 ccagcggaat aagcgcctga atctgtgcct gttcaaacgg ggcaaaatat ttcactccgg
     9721 atgatttttg ccagccacgg ttttccagct caatgccaat agaggtgtcg ttaagacgcg
     9781 ttgccccgcg ccaggcgcta atccccgcat gccaggccag ttcctgttcc ggcaccagtt
     9841 gccagatgcg cggtttaccg ttgtatcgcg gtggtaccgc agggaccaga taatgcgagc
     9901 tgacctgctt atcggtcagt gtcgccagcg agctatcaaa atcatctgcg gtgtagtgga
     9961 taaccagcac tttaatgcgc ggatacgccg cctgcgcctg gcgtcgggta tcaagctgat
    10021 atccctcttt ctcgacaatg cctttttcgc ctgcacaccc tgccagtaac agagcgacag
    10081 cgaccagcca aaagactctt ctcatcgacg cgttttcacc gccgtaccgc tgacgctaac
    10141 catcagcata ctgccatttt gcccgaccgt ttcgtagtcg atatcaatac cgactacggc
    10201 atcagccccc agcgcccggg cctgatcgcc gagctcctca aaggcgatct cccgcgcttt
    10261 acgcaattct ttttcataag ccccggagcg tccgccaacg atatcgcgga taccggcaaa
    10321 gaaatcacgg aaaatattgg cacctaaaat cgcttcgccg gtcaccacac cgcaatattc
    10381 gacgatggtc tggccttcca gagttggggt tgtagaaaat tgcatgatcg atctccttgt
    10441 ttgctaagcc ttcgatcttc aaagcattat cggactgata cgctattatt gaaaggatat
    10501 cattattatc gtagaacgct ttcaaggtgt tcgcttataa ggaaatcatc atgcgctata
    10561 gcaaattgac catgcttatc ccttgcgcac tgctactcag tgcctgtacc accgtcacgc
    10621 cagcttataa agataacggc ccccgcaccg gttcctgtgt ccaggggggg cctgatagcg
    10681 tggctcaaca gttctatgac taccgcattc aacatcgcgg caatgacatt accgctctgc
    10741 gcccctattt gagtgacaaa ctggcaaccc tacttagcga tgccagccgc gataatagtc
    10801 atcgtgaatt gttaagcagc gatccgttct ccagtcgaac caccttgcca gacagcgccc
    10861 acgttgccag tgcatcgact atccctaatc gtgatgcccg taacattcca ttgcgcgtgg
    10921 atctcaagca gggtgaccag ggctggcagg atgaagtgtt aatgattcag gaaggccagt
    10981 gttgggtcat cgacgatgtg cgctacttag gcggtagcgt tcatgccaca gcgggtacgc
    11041 tccgtcagtc catcgagaat cgctaacgtc ttgatcaacc aataaacccc gtaaattgta
    11101 cggcatttcg tgaggaatgc cgacatttat gctcgccgac caccgccccc gttattttgt
    11161 gctatgttta ttgaataatg cgctttgctt ttaactttta aagcagaaat attgcataat
    11221 tattctgtca aaggtactat cttcgacctc aatagctatc agactgccag tatacgagtg
    11281 tcaatgagta ttcaattaaa cggcattaat tgcttctacg gcgcgcatca ggcgctgttc
    11341 gatatcacgc tggattgccc acagggcgaa acgctggtgt tacttggccc cagcggcgcg
    11401 ggtaaaagct cgctgctgcg tgtactcaat ctgcttgaga tgccgcgctc cggtacgctc
    11461 aacattgcag gcaaccattt cgatttcacc aaaaccccct ctgacaaagc gattcgcgat
    11521 ttgcgtcgta acgttggcat ggtgtttcag caatacaacc tgtggccgca tctgaccgtg
    11581 cagcaaaacc tgattgaagc gccctgtcgt gtactggggt tgagtaaaga tcaggcgctg
    11641 gcccgtgcag aaaaactgct ggaacgtctg cgtctcaaac cttatagcga tcgttacccg
    11701 ctgcatcttt ctggaggtca gcagcagcgt gttgctattg cccgtgcgtt gatgatggaa
    11761 ccgcaggtac tgctgttcga tgaaccgacc gccgcactgg accccgaaat tacggcacaa
    11821 atcgtcagca tcattcgtga gctggcagaa acgaatatta cccaggtgat cgtcacccac
    11881 gaagttgaag tggcgcgtaa aaccgccagc cgagtggtgt atatggaaaa cggtcatatc
    11941 gtagaacaag gcgacgcgag ctgctttacc gagccgcaaa ccgaagcatt taaaaactat
    12001 ctctctcact aagattcggg ataacaacaa tgaaaaaagt tctgattgcc gcgttaattg
    12061 caggttttag tctttccgcc acagcagccg aaaccattcg ttttgccacc gaagcctcct
    12121 atcctccgtt tgaatcgatt gatgcaaaca accagatcgt tggttttgat gtcgacctgg
    12181 cgcaggcatt gtgtaaagag attgatgcaa cctgcacttt ctctaaccag gcgtttgaca
    12241 gcctgatccc aagcctgaaa ttccgtcgcg tagaagcagt gatggcgggc atggatatca
    12301 ctccggagcg tgaaaagcag gtgctgttta ccaccccgta ctatgacaac tctgccctgt
    12361 ttgtgggtca gcaaggcaaa tacaccagtg ttgatcagct gaaaggcaaa aaagtcggcg
    12421 ttcagaacgg gacgacacac cagaagttca ttatggataa gcatccggaa atcactaccg
    12481 tgccatatga cagttaccag aacgcaaaac tggatctgca aaacggtcgt atcgacagcg
    12541 tatttggtga caccgcagtg gtaaccgaat ggctgaaaga taacccgaaa ctggcggcag
    12601 tgggcgataa agtgaccgat aaagattact tcggtactgg cctcggcatc gcagtacgcc
    12661 agggcaacac tgaactgcag cagaaactca acactgcgct ggaaaaagtg aagaaagatg
    12721 gcacttacga aaccatctac aacaaatggt tccagaagta attcctgatg aatgaatttt
    12781 ttcctttagc aagcgccgcc gggatgaccg tcggccttgc cgtttgtgca ttgattgtcg
    12841 ggctggcgct ggcgatgttc tttgcggtat gggagtctgc aaaatggcgt cctgtcgcgt
    12901 gggcaggttc agcgttggtg acggttctgc gcggcctgcc agaaattctg gtggtgctgt
    12961 ttatctattt tggctcctcg cagctgctgc tgacgctttc ggatggcttc actatcaatc
    13021 ttgggttcgt gcagatcccg gtgcagatgg acattgagaa cttcgacgtt agcccgttcc
    13081 tttgtggtgt catcgctctg tcactgctgt atgccgccta tgcctcgcaa acgctgcgcg
    13141 gcgcgttgaa agcggtgccg gtgggccagt gggaatctgg tcaggcgctg gggctgtcga
    13201 aatcggctat ctttttccgt ctggtgatgc cgcagatgtg gcgtcatgcg ctgccgggtc
    13261 tcggtaacca gtggctggtg ctgctgaaag ataccgcgct ggtcagtttg attagtgtga
    13321 atgatttaat gctacaaacc aaaagcatcg ctacccgtac ccaggaaccg tttacctggt
    13381 acattgtggc ggcggcgatt tacctggtga tcaccctgct cagtcagtac attctcaaac
    13441 gcattgacct gcgcgcgaca cgttttgagc ggaggcccag ctaatgtttg agtatttacc
    13501 cgaactgatg aaagggttgc acaccagcct gacgctaacc gttgcctcgc tgattgtggc
    13561 actgattctg gcattgattt ttaccatcat cctgacgctg aaaacgccgg tgctggtgtg
    13621 gctggtgcgg ggctatatca cgctgtttac cggtacgccg ctgctggtgc agatcttcct
    13681 gatttattac ggaccgggcc agttcccgac gttgcaggag tatccggcac tgtggcattt
    13741 gttgtcagaa ccgtggctgt gtgcgctgat tgcgttgtcg ctgaacagtg cggcgtatac
    13801 cacgcagctg ttttacggtg caattcgtgc gatcccggaa ggtcagtggc agtcctgtag
    13861 cgccctggga atgagcaaaa aagatacgct ggcgatcctg ctgccgtatg cctttaaacg
    13921 ctcgctctct tcttattcca acgaagtagt gctggtgttc aaaagtacct cgctggccta
    13981 caccattacg ctgatggaag tgatgggata cagccagttg ttgtacggac gcacctacga
    14041 tgtcatggtg ttcggcgcgg cagggattat ttatctggtc gttaacggcc tgctgacgct
    14101 gatgatgcgt ctgatcgagc gtaaagcgct ggcgtttgag cgaaggaatt aatttggctg
    14161 cgtgctgatg ccggatgcgg cgcggacgcc ttatccggcc tacatagtgc ataaattctt
    14221 ataatgaaga cggacaactc actaagttgt ccgtcttttt ttatttcatt taaattattt
    14281 aatcatattt attgcatata aattcacttg atggcattgt tatcccatgc cgcagacacg
    14341 gccaaaaatc ataagattga cagacgggag ttccatcatg aaaaagttag ttcttgccgc
    14401 attacttgct tcctttactt tcggtgcttc tgccgcagag aaaatcaatt ttggcgtttc
    14461 agccacctat ccaccctttg aatctatagg tgctaataat gagattgtcg gctttgatat
    14521 cgacctggca aaagccttgt gcaaacaaat gcaggcagaa tgtactttta ctaatcacgc
    14581 gttcgacagc ctgatcccgt cactgaaatt cagaaaatat gacgccgtaa tctccggtat
    14641 ggatatcact ccagagcgta gcaaacaggt atcgtttacc acgccctact atgaaaactc
    14701 agccgtcgtg attgccaaaa aagataccta caaaacgttt gccgatctga aaggcaaacg
    14761 tattgggatg gaaaacggta ctacgcacca gaaatatatt caggatcagc acccggaagt
    14821 gaaaactgtc tcctatgaca gttatcagaa tgcctttatc gatctgaaaa atggccgtat
    14881 tgatggggta tttggtgaca cagcggtggt taatgaatgg ctgaaaacca acccgcaact
    14941 gggcgttgcg actgaaaaag tgaccgatcc gcaatacttc ggcaccggcc tgggtatcgc
    15001 tgtacgtccg gataacaaag ctctgctgga aaaactgaat aacgcgctgg cagcaattaa
    15061 agctgacggt acatatcaga aaatcaatga ccagtggttc ccgcagtaag attcagaaag
    15121 ccgcacctgc ggctttttta ttgcttaacc agcagtgtca gcacttcgta atgcgcagtg
    15181 tgcgggaaca tatcaaaaag ctgtacccgt tcgatgcagt aacctggcag ttcgcggata
    15241 tctttcgcca tggtttgggc gttacagctg gagtagataa taaaacgcgg tgccatcgtt
    15301 gagagataat cacacagcgg tttaccaatg ccgcggcgcg gcgggttaac cagcaccagc
    15361 tccggcacct ccccctgagc agtggcaaac tgagtggagt ccagcgcctg aaattgcaaa
    15421 cgcgttaagc ccagttcagc ggctgactgc tttgcacagg caatggcctc tggcgcaatt
    15481 tcgatcccgg ttaactgcat gtcaggcatc gcgcagtgta aaccaaagcc ccccacaccg
    15541 cagaacagat cccacatatg tttaaccgac agctgtcgta cccagtcgcg cgcggtggcg
    15601 tacaactggc tggcgaccgc cggattagtc tggaagaaac tttgcggacg gatccacagc
    15661 ggtacgtcat taaaacgctc tgccagcgcc tgttgttcag tcaggtaaat ctccgtctcc
    15721 ccttccataa tcgccatatg taccggctga atattgacgg taataacttt cagctgcggt
    15781 agttgttcct gtaaccacgg cagcgcctta cgcagttgcg ccagcttggt atcagaacgc
    15841 agtacaaagc gcagcatcat gccgccatcg ctctggcttt cagtcagcag aatgtatttc
    15901 agttcgccac gcttacgcgc cacgttgtag ggcgttaacc ccgctcgggc gataaacggt
    15961 tttagcgccg caaaaacggg cgcaaatgag gcaggataaa gcggacagtc acaaaggtct
    16021 tccggcgtgc cgtcgcgatg cagcataccg agcagtggtt tttcaacgct accactcacc
    16081 accattttgg ctttattacg aaacccttgt tccgggcctg acaccggcgc gcaccattcc
    16141 tcaaccggaa agtcggcgag cagattttta agatcggcgg ttttagcgga gagttgctct
    16201 ggaatcggct gcgttatcca ctgacaggaa cgacagcgac ccgcgtcgta aagtgcgcac
    16261 tgcatacatt gaccttcaca tcatcagggg cgacgattat acacattatt gcaactggaa
    16321 gaaccgccga ctggtcgagg gaacgaacag cagcatcagg atgagcatat ccggcagctt
    16381 ctgcagcatc aggctatgga agatttcacg ttttgattca ccgggaatgc tgaacagctc
    16441 cggataacca taccccagcg aagctgccca caagtaactt gcggccgtga tttgtgtcaa
    16501 cagatacagc cagcgcgccc agcgacgccc tttcaccagt gaaaacgcac accagatctc
    16561 aatgaaaacc agcaccagac tgctcaaaaa gaccagcgtt aaactccagg tttgtacgct
    16621 gcgatgaatg aactcgccaa taccgcgcac gcccagcgta ttgaaaatca tcagcacgtc
    16681 gaggccacgg atcataataa tggcgagcgc cgccacctgc accagcgcag gcacattcag
    16741 gcgagcatga gatgaagatg ttttcttaaa aaatcccaac gtttcgtctt ccatgaaaac
    16801 aatgccgcga catgcgcggc attatgtagc caggttggca aattttagtg tcttcagcca
    16861 cgtcttgcac gctggatgtc gcgtatccgc tgtttttctg cgcgcgccat cagataccag
    16921 gcgataaatc cgacaattcc gaccgcgccg aggatcaatg ttgccagggc gttgatttcc
    16981 ggattcaccc ccatccgcac gctggaaaag accagcatcg gtaaagtggt ggctcccggc
    17041 ccggaaacaa agctggcgat caccagatca tcaagcgaca aagtaaaagc cagtaaccag
    17101 ccagaaatga tcgcgggcat gatcatcggt agcgtaatga caaaaaacac tttcagcggc
    17161 gtcgcaccga gatccatcgc ggcttcttct atcgagcgat ccagttcccg cagacgcgac
    17221 gaaataacga ctgccacata agccgtacag aaagtgacat gcgccagcca gatggtgagc
    17281 ataccgcggt ccgcaggcca gccaatagca tgagcaagcg cgacgaataa caacaatagc
    17341 gacaagcccg tgatgacatc tggcatcacc agcggcgcgg tgatcataaa ggcaaaacca
    17401 tttgatccgc gaaacctacc aaagcgcacc agcacgaccg ccgcaatcgt cccgaggatc
    17461 gccgccgccg tcgccgcaca ggccgcaatt gtcaggctta aaccaaccgc actcatcatc
    17521 gcgttatcgc gcaataactc accataccag cgtgttgacc agccggccca caccgtcacc
    17581 agtttcgagc tgttgaatga atagatcacc agcatcagca ttggcgcgta gagaaaggtg
    17641 aagcccagca gcaaaatcac aatccgccag ggcgaacgaa caaccggtaa attattcatc
    17701 cgtgttctcc cacgcttttt tgctggtgtt tgtgaaacca cattatcggc acaattagca
    17761 gcagcaacat gatgatcgct accgccgagg ccaccggcca gtcgcggtta ttgaaaaact
    17821 cctgccacag cacgcgcccg atcatgatgc tgtccgggcc accgagcagt tcagggatca
    17881 caaactcgcc cacagccggg ataaacacca gcatcgatcc ggcaataatc ccgcctttgg
    17941 tcagcggcac gatcacagta aagaacgttt tcagcggtcg tgcaccgaga tccagtgccg
    18001 cttccaccag cgaataatca atacgaatca aggcggtgta gatcggcagc accataaacg
    18061 gcacataggc gtaaacaatg ccgatataaa ccgccaggtt ggtatgcaga atggtcaacg
    18121 gttgatcgat aacccccagc cacagcagaa aattattcag cacaccgttg ttttttaata
    18181 ttcccatcca ggcataaacg cggatcagaa acgaggtcca cgacggcagg atcaccagta
    18241 gtaataagat attacgggtc gaaggcttac tgtgcgccac cgcccacgcc agcggatagc
    18301 cgatcagtaa acagcaaaat gtcgaaatcg cagccacctg tagcgactgg agataagcat
    18361 cgaaatagag cggatcgtca gtcagttgca gaaaattacc gagattaaga gtgatggaca
    18421 gttgcccgtc agcccactcc atcagttcgg tataaggcgg aatggcgcgc gccatctccg
    18481 ccagacttat tttaaagacg atcagaaatg gcagcagaaa cagcaggatc aaccagatat
    18541 acggcaacgc aatgaccagt ttgcgcccat gcttcatttg cagctgcgac aaccacagct
    18601 taaatccgcc cggcggtttg ggctgggcag caggttcaag tgtactcatc tttgctcctt
    18661 aaaccgtcag caccacacag ctgtccactt cccagcacaa acgcacttcg tcgccccagg
    18721 ttggtaaccc tttgcgatat cgatgagcgt tttgtagctg agcgctgatc atctgtccgc
    18781 tcttgagacg aacgtgatac accgaaagat cgccgagata ggcaatgtgc atcacctcac
    18841 ctaccgcaaa gttaaaaccg tcagcgggag gttcctcgca aagcatgatt ttttccgggc
    18901 gcagcgccac atgcaccggc acattatcga ccaccgaagc atctacatcg actttcagtg
    18961 gatgcaccag ccccggcgaa taaagcacca gaccatcttc ctgacgctct ttgagtacac
    19021 cttcaaagac attaaccgag ccaataaatt cggcgctgta gcgagtggtc ggatgctcgt
    19081 agatctcttc cggttcgcca atctggacaa atttccctcg attcataatg gcgatacgcc
    19141 ccgccatggt catcgcctct tcctgatcgt gggtgaccat cacacaagtc acaccgacac
    19201 gctccagaat atccaccact tcaagctgca tccggtcacg cagcttttta tccagcgcgc
    19261 ccataggctc atcgagcagt aatagtttcg ggcgcttggc aaggcttcgg gccagtgcca
    19321 cacgctgtcg ctgaccgccg gaaagctgat gcggtttgcg tttggcgaac tcctgcatat
    19381 gcaccagccc gagcatctca ttgacccggc tggcaatttc cgctttcggt agtttgtcct
    19441 gtttcaggcc aaaagcgatg ttctgttcca cggtcatatg cggaaacagc gcgtaagact
    19501 gaaacatcat atttatgggg cgcaggtaag gcggaacctg tgacaaatcg acgccatcaa
    19561 gcattatctg tccggcagaa ggttgttcga aacctgccag catacgcagc agcgtggact
    19621 tgccacagcc ggatgcgccc agcagcgcga agatttcgcc tttatagatg gtcaggctga
    19681 catcatcgac cgcatgctga ccatcgtaag atttagtcag attacggatt tctaatagcg
    19741 gcgtcagcac cttacgggtt ttcgcctgcg ggcgagggat agcgtcattc acggctgctc
    19801 tccggcattg aatgtgtgca agcgcaccat aatggtgcga attgccggcg ggcgtggtgc
    19861 gcccgtccgg catctacgac tgcggattat tttccgctct tcactttggt ccacgcgcgg
    19921 gtgcgcacac ggtcgatttt cggatcctgt actttcagag tgaacagctt cgcacgaaca
    19981 tccgcaggcg ggtaaatacc cgggttatca cggacttccg cactcaccag cggcgtagct
    20041 gctttattgg cgttagcata gaacacatgg tcggaaatat gcgccaccac atccgggcgc
    20101 agcaggtaat tcaggaactg ataggcttcg tctttatttt tggcatccgc aggcatggcg
    20161 aagacatcaa agaacgccat cgccccttct ttcggaatcg agaacgagac attaacgcca
    20221 ttcttcgctt ccttcgcgcg gtttgacgcc tgccagacat cgcccgccca gccaatagcg
    20281 acgcagatat cgccgtttgc caggtcgtta atgtattgag atgaatggaa ataacgaatg
    20341 ttcgggcgca gctttaacag cagatcggtt gccggtccgg tgtaatcatc tgctttagtg
    20401 ctgttgggat ctttaccgag ataattcagc acggtagcaa acacttcttc cggtgcatca
    20461 aggaaagaga cgccgcagct tttcagtttt tccagatttt caggcttgag gatcaagtcc
    20521 cagctatcga cgggcgcgtt ttcgcccagc accgctttaa ctttatcaac gttatagcca
    20581 atcccggtcg tcgcccacat atagggcata gcaaatttat tgtcgggatc gtgtttggca
    20641 actaacttca gcagctccgg atcgagattc ttccactccg gcaatttgct tttgtccagc
    20701 ggctggaaaa ctcccgcagt caactggcgt tcaagaaagc ttgctgacgg caccaccagg
    20761 tcaaagccgg tgctacccgc cattaatttg ccttccagta cttcgttaga gtcgaaaaca
    20821 tcgtagacga ctttaatacc ggtttctttt tcaaaattgg ccaccgtgtc cggggcgata
    20881 taatcagacc agttataaat gtgaagtgtt ttttgttcag ccgcgagcgt gccgacagag
    20941 acggccatca gagcacccgt aaccagaccc gatagccatt ttttatttaa ggcggtcata
    21001 tctctttcct tctgaaagtt cgttaacaaa cgaacaaaag ttgtcacact gaaagatatg
    21061 caataaacgt gcatattttt tatcggcgcg gtgtaagaaa agagaactct ctcccggcca
    21121 gcactgctcg ctatataaaa gcatcgcaag aataacttta gccagggttt gagactatag
    21181 ctcggattaa aaaacggtat ttaacaaatt tttatgcgta tttgctctat gtgataaagc
    21241 caaatcggca caagagaagt gaaataatct ttaagaaaag gttaagaata gcagataatt
    21301 ttgcagcacg cagtcactgc ggcagcgact gcgtactcga aaggagttat cagtggagga
    21361 aatagttttc ggtaacgttg ttttgccctt catcgtcagt cggtaacagc agttgatggg
    21421 cattagcttc aagaatgacc atcgaaatct gctcttcgct ctgacgcacg aaccatgcaa
    21481 actgctcata ggttacgccc tgcataacgg ataaagactg gcaaaccacc agctttggca
    21541 gattatcatc ctgcatatca agaaatgctt tcacggtcag cgaactggca ttgatggcag
    21601 ataaatccgc cgccagcggc aatacggctg aaggtcggac ttccgccatg gcagaaaaca
    21661 ggatcgtgtt atcgatcaga tcgattttgg catcaaagac accgtcgaaa ttctgcatat
    21721 ggggcagatg cagagcctga cagttatcac attcaaaaaa actcatcccc aggtcatcga
    21781 gccattgacg cagcgtatcc agaccaggaa cgaccagcga tgtcataatt ttgtacaacc
    21841 tctttcgata aaaagaccgg cacagcttac gcaaaaagcg caggcaaaac catgatcagt
    21901 aatgtgattg cgactaacca cccgttttca ggcaatattc cgtcgtagcg tggcgttcga
    21961 tccagcggat cattttaccc gcgatgtcga taccggtggt tttttctatt ccttccagcc
    22021 ccggcgacgc attcacctcc atcaccaatg gcccgcgatt agcacgcaga atatccacac
    22081 cagcaacgtc cagcgccatc gttcgcgcgg cttttatcgc gatttcacgc tcctgtggtg
    22141 tgatactggc gacgcttgcc gcgccgccac gatgcaaatt ggaacgaaaa tcgccctctt
    22201 tcgcccgccg ttcaatcgca gcgaccactt catcgccaac aaccagacag cggatatcgc
    22261 acccttgcgc ctctttgata tattcctgca ccagaatatg cgcgttcaga ccgcggaaag
    22321 cgtcaatcac gctttccgcc gcctggcgcg tctccgccag cacgacacca attccctgcg
    22381 tgccttcaac cagcttgacc accagcggcg caccaccgac catgtcgatt aaatcgctgg
    22441 tatcatccgg cgaatgcgca atgcccgtga caggcaggtc gatgccctga cgcgccagca
    22501 gttgcatgga acgcaatttg tcacgcgccc gggcaatggc gaccgactca ttgagcggat
    22561 agctccccag catctcgaac tggcgcagtg ccgccgtccc ataaaaggta atggcggtac
    22621 caatacgcgg gatcacagca tcaaaatggg gtaacttgcg gcctttgtag tgaatagaag
    22681 acgccgcagg atttatgttc atgtagcaag aaagcggatc aagaatttca accaggtgac
    22741 cgcgctgtat agcggcttca cgcagccgct tacacgaata gagcgttcca tcccgggaca
    22801 atatggcaat tttcaccctg cacctctctg tcagacctgg tcaaaagccc gcgtatcata
    22861 catcggcgtg gcggttttag cgcgtcgccc aaccctgttt gtgcaaataa tccagtataa
    22921 atgggcggct ttctttaatg attgttcggc ggatatgatc gctccaggta tcccggcgat
    22981 tattgctgcc acgggtgagg taatattccg ccagttgttc gtcatactgc gccagtgcgt
    23041 ctttatccag cggttgatag ttgttttcat gcaccaaaat ggaggccggt aaacgcggct
    23101 taagatccgg attatccgca ggccagccaa ggcacagccc aaacagcggc agaacatgct
    23161 gcggtaattt cagcagctcc gtcaccgctt caatattatt gcgcaggccg ccgatatata
    23221 ccccgcccaa tcccagcgat tccgctgcgg ttaatgcatt ctgcgccatc attgccgtat
    23281 caacgacacc gagcaacagt tgttccgcca ggccaagttg tgcatccgga cagatctgta
    23341 aatggcggtt aaagtcggca cagaacaccc agaactccgc cgcttgcgct acgtgttttt
    23401 gcccgccggt cagcgtcacc agttcatcac gtaacgcttt gtcggtaatg cgaataatgc
    23461 tactgcactg caaaaaactg gaactggacg tcgcacgggc gctgttaata atcgcctcac
    23521 gctgcgcttc ggaaatgggt tcatcagtga aatggcgaat ggagcgatgg ccacaaataa
    23581 gttcaatggt tggcgtcatt atctttttct ctttctgaac gtgaatattg cggtggacgg
    23641 ttcatcagct gtggggcaag acgttttgcc acctgaagaa taaccaccac cgcagcggga
    23701 agcatgagca aaacaccgag aaaaatcatc agaatctgca cttctggccg agaaaatggc
    23761 tcaggcagcg acagggagcc gcttaccgac agcagcgcca ccgccagcaa catcattccg
    23821 ataaattcca gtatcaacac gcttttaggc aatttaccga tcgcgcgcat acgcttccct
    23881 ctgcaaagtg agccttcagt ctaaaacttt tcactgtatt gtgtttaaca gttatagctt
    23941 ttagcaatta atgcaacagg ttaaacctac tttcagcgaa tacattttag cgtgatcatt
    24001 acaggtataa atctatgagg agagaaataa tgcaaaccgt tatttttggt cgtccgggtt
    24061 gcccttactg tgtgcgtgca aaagatctgg ctgagaaatt gagcaatgaa cgcgatgatt
    24121 tccagtatca gtatgtagat attcgtgcgg aagggatcac taaagaagat ctacaacaaa
    24181 aggcaggaaa acccgtagaa accgtgccgc agatttttgt cgatcagcaa catatcggcg
    24241 gctataccga ttttgctgca tgggtgaaag agaatctgga cgcctgatcg tctgacaagc
    24301 cctcgcgttg agggctttat tgattttttc tgtgctgtgg tttaaacaaa ctactgataa
    24361 ataagaaaca caatgccccc agcgcacacc agaacaccgc acttaataac catgccagct
    24421 cttgccagaa tgagcgcgtc ggtgaaaaaa acagccgcat aatgagcatc gaacagggtg
    24481 ccgccagcat tgcgccaaac agaggtttca ggacttctct acgatgtgaa aagaagctgg
    24541 caactgcacc aggaagaatg aaaaatagca aaccgatttc aggatgcccg gcagcccgaa
    24601 aagcgccttt catgtgcgtc gccagaaaaa gacacaccac aatgaagagg acaaaacagc
    24661 agattgcccc cgcccaacgt tgtttatgtt tcactcgttc ctcctgacac tgcgtctatc
    24721 gaacacattt ttcgccagtg tggcgttcag taagataaag ccgcttcgca ttccatgcta
    24781 atataggcca acgcaattca tatagccgtt gatacctaat gtgattacac tagtaaaata
    24841 tattgttact ttactatcgt ttaggtgcgc tgaatgaatc tgcgccctga attctggtaa
    24901 aaaacattat cgtaaattac catttctttc aacagcttac tagtaaacaa gaagttagcc
    24961 tccgtgaata taaacgtcgc cgaattgtta aatgggaatt acattctgtt attatttgtg
    25021 gtcctcgcgc ttgggctatg tctcgggaaa ttacgacttg gttcgatcca actgggtaat
    25081 tccattggcg ttttagtcgt atcgctgtta ttaggccaac aacatttcag cattaacacc
    25141 gacgcgctta atcttggctt tatgctgttt attttctgcg tcggggtcga agccggaccg
    25201 aacttttttt ccattttttt tcgcgatggg aaaaattacc taatgttagc actggtgatg
    25261 gttggcagtg cgctggtcat cgccttaggg ttaggtaagc tgtttggctg ggatattggc
    25321 ctgacggccg gtatgttagc aggctctatg acgtcaacac cggttctggt cggtgctggc
    25381 gatacactgc gtcattccgg catggaaagc aggcagctct cattggcact ggataatctg
    25441 agcctcgggt atgccttaac ctatttaatc ggcctggtga gtttgattgt tggtgcgcgt
    25501 tacttgccga aattgcagca tcaggactta cagaccagcg cccagcaaat cgcccgcgaa
    25561 cgtggcctgg acacagatgc caaccgtaag gtttatttgc cggtgatccg cgcctaccgc
    25621 gtcggcccgg agctggtagc ctggaccgac ggcaaaaatc tgcgtgaact gggtatttat
    25681 cgacaaactg gctgttacat tgaacgtatt cggcgtaacg ggattctggc aaatccagac
    25741 ggtgatgccg tactacaaat gggcgatgaa atagcgttgg taggctatcc tgacgcccac
    25801 gcccgactcg atcccagttt ccgtaatggc aaagaagttt tcgatcgtga ccttctcgac
    25861 atgcgtatcg tcactgaaga agtggtcgtt aaaaaccata acgctgttgg caaacgcctg
    25921 gcacaactga agttgaccga tcacggttgc ttccttaacc gcgtcatacg tagccagatt
    25981 gagatgccga ttgatgacaa cgtcgtgctt aacaaaggtg acgttttaca agtcagcggc
    26041 gatgcccgcc gggtaaaaac catcgccgat cgcatcggct ttatctcgat tcacagccag
    26101 gtcactgact tgctggcatt ctgcgccttc tttgttattg ggctgatgat cgggatgatc
    26161 accttccagt tcagcacatt cagtttcggt atggggaacg ctgccgggtt gttattcgcc
    26221 ggaattatgc tgggctttat gcgtgctaac cacccgacct tcggttacat tccgcagggt
    26281 gcattaagca tggtgaagga gttcggcttg atggtgttta tggcaggcgt tggtctgagc
    26341 gctggtagcg gtattaataa cggcctgggc gcgattggcg gtcagatgtt gattgccgga
    26401 ttgattgtca gtctggtgcc agtggttatc tgcttcttgt tcggtgctta tgtattgcga
    26461 atgaaccgcg cactgttgtt cggcgcaatg atgggcgcac gcacctgcgc gccggcaatg
    26521 gagatcatca gtgatacagc tcgcagtaat atcccggcac tgggctatgc gggcacttac
    26581 gcaatcgcca acgtcctgct gacgctggca gggacaatca tcgtcatggt atggccagga
    26641 ttaggataaa actgaagttg ccctgaaaat gaaatttttt ttgcacaacc gcagaacttt
    26701 tccgcagggc atcagtctta attagtgcca ctgcttttct ttgatgtccc cattttgtgg
    26761 agcccatcaa ccccgccatt tcggttcaag gttgatgggt tttttgttgt ctaaaattta
    26821 tctactaccc tgcaaccctc tcaaccatcc tcaaaatctc ctcgcgcgat agcggcttac
    26881 ggtcggtgac aaaatgcagc gtcatcccct caataaacgc atcaagcgcg cgggcggttc
    26941 cgggttcaaa ccattgttcg agcgtttgct gactgcgctg catccagttt tgcattaccg
    27001 tttttaatag cggttttcgg ctagccagcg cgtagagctg gtacatcagc tccatgttat
    27061 ccggcgttgc aacctgtgag ctgtaaatca tatcggtgat agcctggcat gcgcccggag
    27121 catcactaac atcgctaaaa aatgcctgat attgccggga catgatctca gtaaaactgc
    27181 tgaacgcctc cagcaacaac tcatcaattc ctgaaaaata gtaggtcatc gaccccaacg
    27241 gtaccccggc aagggtagca attttgcggt gcgtaacagc atgtattcca taaagtttca
    27301 ccgcctccag cgtggcctgg ataatttttt ctcgccgttg cggatcgtta gcgcgacgca
    27361 tgtcatttcc tctctctgca attgtgtaca aatgtacaca accttgctaa tgttgtcttc
    27421 cctcttctta ttttgacgtg atctatgacc gtaaattctt cacgtaatgc attgaaacgc
    27481 cgaacctggg cgctgtttat gttcttcttt ttgccaggcc tgttaatggc gtcctgggca
    27541 acccgtacgc ctgctatccg cgatattctt tctgtttcga tcgctgaaat gggcggagtc
    27601 ctctttggtc tgtcgatcgg ttcaatgagc ggtattctct gctcggcgtg gttagtgaaa
    27661 cgctttggga cgcgtaatgt catcctggtc acgatgtcct gcgcattgat cgggatgatg
    27721 atactaagtc tggcactctg gctgacatcg cccctactct tcgcggttgg tctcggcgtc
    27781 tttggggcaa gttttggttc tgcggaagtg gcgataaacg ttgaaggtgc tgccgttgag
    27841 cgagaaatga ataagacggt tttgccgatg atgcacggtt tttatagcct gggcacgctg
    27901 gcaggcgctg gtgtcgggat ggcactaacg gcctttggcg ttccggcaac ggtgcacatt
    27961 ttattggcgg cgctggtagg catcgcgcct atttatatcg ccattcaggc aatccctgat
    28021 ggtacgggca aaaatgctgc cgatggcacc cagcatggtg aaaaaggcgt acctttttat
    28081 cgcgatatcc agttgctgct gattggtgtt gtggtgctgg cgatggcctt tgccgaaggt
    28141 tctgccaacg actggttacc cttattaatg gttgacggtc acggttttag ccctacttcc
    28201 ggctcgctga tttatgccgg ttttaccctg gggatgaccg ttggacgctt taccggcggt
    28261 tggttcattg accgttacag tcgcgttgcc gtggttcggg ccagtgcact aatgggggcg
    28321 ttgggtattg ggctgattat ttttgtcgat agcgcctggg ttgctggggt gtctgttgta
    28381 ctttggggac tgggtgcctc gttgggcttc ccgctgacca tttctgccgc cagcgatacc
    28441 ggccccgatg caccaacccg cgtcagcgtg gtagctacga ccggttatct ggctttcctc
    28501 gtcgggccgc cactgctggg ctatctcggc gaacattatg gattacgtag tgcaatgctg
    28561 gttgtactgg cgctggttat tctcgcggct attgtcgcga aagccgtcgc caaacccgat
    28621 accaaaacgc agacggcgat ggagaatagt tgatgagcat taaattaatt gcggtagaca
    28681 tggatggtac tttcttaagc gatcaaaaaa cctataaccg tgagcggttt atggctcagt
    28741 atcagcaaat gaaagcacaa ggcattcgct ttgtggtcgc cagcgggaat caatattatc
    28801 agttgatctc tttctttccg gaaattgcta atgaaatagc ctttgtggct gaaaacggcg
    28861 gctgggtagt gagcgaaggc aaagatgttt ttaatggcga gctgtcgaag gatgcgtttt
    28921 ctactgtcgt ggaacatttg ctgacgcgcc cggaagtgga aattattgcc tgcggaaaaa
    28981 atagcgccta tacactcaaa aaatatgacg atgccatgaa aacggtggcg gaaatgtatt
    29041 atcaccgtct ggaatacgtc gataactttg acaacttaga agatatcttc tttaagtttg
    29101 gtctgaatct ttccgatgaa ctgattccac aagtacaaaa agcattacat gaggccatcg
    29161 gcgatattat ggtgccggtc cacaccggca acggcagcat cgatctgatt atccccggcg
    29221 tacataaagc caatggcctt cgccaactgc agaaattatg gggaatagac gacagcgaag
    29281 tggtggtctt tggcgatggc ggtaacgata ttgagatgct gcgtcaggca ggctttagtt
    29341 ttgcaatgga aaatgccggc agcgcggtcg tcgcagcggc aaaataccgg gcaggctcca
    29401 ataaccgtga aggcgtactg gatgtgatcg ataaagtgct taaacacgaa gcgccattta
    29461 accaataaaa cggcagcacg cccggcaaca caatacctga atgaaggctg cataattcgc
    29521 ggaaattaac ccgctgcatt atttcaggcg aaatgattat gaaaaattgt ctactgttgg
    29581 gcgcgctttt aatgggcttt actggtgtgg cgatggcgca aagtgtcacc gtcgatgtgc
    29641 cgagtggata caaagtggtt gtggttcctg attcagtcag cgttccacaa gcggtcagcg
    29701 tcgcgactgt gccgcaaacc gtctatgtgg ctcctgcgcc agctccggct taccgcccgc
    29761 atccttatgt tcgccatctt gccagcgtag gtgaagggat ggttatcgaa caccagattg
    29821 acgaccatca ccattaattc gagaatgcct gatcgcacaa agcaatcagg cattttttta
    29881 cccttcgtgc gaatttccca tctgtttatc ttttaaaaag ataaccatca gcaacagcca
    29941 caaaattccg ttgacaaggt tgaagagatt aaacagtccg ttgcccccgt tcagccagac
    30001 atgtttgctg atttcaatac cgacggtaaa gatcagcatt tgcagcatcc ccatcgccgc
    30061 agaaaccgta cctttactca tatcgctggc aaacagggtt aatcgcacca gtcccgcatt
    30121 cgccagacca ataccgaaag cataaatact taacccggcg gtcatccata aatacgcgtg
    30181 cgatgagata accgttgccg cagcagcgac caacagacca atcataatcg gccagccgcc
    30241 cataataatc agcgaacgta cggtgcggcg cgaggtcaga cgcgctaaca gcaagttacc
    30301 cgcaattaac gccccgaaaa taggcacttg cagcaagcca tattcatagc tgctcaactg
    30361 ctcaccggta ataatgataa tcggcgactg ggcgatccac gccaacaacg gcaggctaac
    30421 gaatcccagc gccagcgccc ccgccacaaa gcggccgttc ttcagcacca gcttatagtc
    30481 acgaccgagt tctttcagcg acagtttctc gcctatacgc gtggcggttt caggcatggc
    30541 tcgttgcaga ccgaaaaagg agatcgctgc caatgcggca aacaagacaa acatcccttc
    30601 ccagggcagc acatggatcc acgccgcgcc caccagcgga ccaagtagtg gagcaatcag
    30661 cgccacgttc gccattagcg cggtgatctt gatacaaacc gcctcttcga aggattcctg
    30721 aattgcggcg tatcccacag cgccaatgaa acagaggctt atgccctgca agaagcgcaa
    30781 cagggtgaat tgttcaatgt tttgcgccag taatattgcc agacaggtga cgataaacca
    30841 caccactccc gccagcatca ccggacggcg gccaatacga tccgacaacg gccccaggag
    30901 ccattgtaaa aacatcccgc ccgccagata cgcagtcatc gaagtaggaa cccaatcaat
    30961 gcccgcctga tattgttcca ccacggccaa cataccgggt tgaatcatat cgttgccgat
    31021 ataggttgaa aattcgtaaa gcaccagaca gagagggaaa agtaacgcct gacgtccaag
    31081 cctggcaccg gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcaa
    31141 agtttaataa aagcgcagct aacgagaaag cgaattttgt agctgaaacc acggttaagc
    31201 acattcttac attattgcga gtatagctac gctttcttta agttttattt aacctttgcc
    31261 cgttacaatc acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc
    31321 cagtttaacc tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt
    31381 tgcgtcggga agttatgctg gaaaatttga atctctctct attctctctt attaacgcga
    31441 cgccagactc tgcgccgtgg atgatctcgt tggcgatttt tattgcaaaa gatttgatta
    31501 ccgtggtgcc gttgctggcc gcggtacttt ggctgtgggg gcttacagca caacggcaac
    31561 tggtgataaa aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg tcgatggggc
    31621 atctttttcc gcacgaccga ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg
    31681 cggcggatga ctcattcccg agcgatcacg gtacggtgat tttcaccttt gcactggcat
    31741 ttttatgctg gcatcgcctg tggtccggct cacttttaat ggtgctggcc gtcgtcattg
    31801 cctggtcgcg cgtttatctt ggcgtccact ggccgctgga tatgctcggt ggattgctgg
    31861 caggtatgat tggctgcctt agcgcccaga ttatctggca agcgatgggg cataaactct
    31921 atcaacgtct gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg
    31981 tgcgtgactg atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca
    32041 gagggattat ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac
    32101 gcagtgataa gttacatctt aaagacgccg ccgccctgct tggggtttcg gagatgacga
    32161 ttcgtcgcga tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc
    32221 tggaaccacg cagcgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag
    32281 aaaaacgccg ggcggcaaaa ctggctgcga cgctggtaga acccgatcag accctctttt
    32341 ttgactgtgg caccaccacg ccgtggatta ttgaagcaat tgataatgaa atccctttta
    32401 ccgccgtttg ttattcgcta aatacctttc tggcgctgaa agagaaaccc cattgccgcg
    32461 cgtttctttg cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc
    32521 agcaaacgct gaataatttt tgcccggata tcgcttttta ttccgctgcg ggcgtgcatg
    32581 tcagtaaagg cgctacctgc tttaatcttg aagagttgcc ggtaaaacac tgggccatgt
    32641 cgatggcgca aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtgcgtccgg
    32701 cgcgcatggg tgacctgaaa cgctttgata ttgttgtgag cgattgttgc ccggaagatg
    32761 agtatgtgaa gtacgcgcag acgcagcgta ttaagttgat gtattaatga cgtataaccg
    32821 gatgacgttt cgcgccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg
    32881 atggaatttc atcatcacga aatcccacat ccgaccaaag aatccgccct cttccacatt
    32941 ttccatcacg atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt
    33001 gccgacaacc tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtataact
    33061 cgccttcagg tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc
    33121 gagattcact tcgctcttat caccaaacca gacgcgctga gtcacaaatg tggcatcagg
    33181 tttaattggc gtcacggttt caaagaaacg gaagccccag gtcaataatt tctcagactc
    33241 attaaaacgg atacggtcag ttttcgcccc cagcactacg gagattaaac gcatatcgcc
    33301 ctgggtagcc gaagcaacca gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc
    33361 atcaacattc agattgctgc tccacagcag acgattacgg ttaggctgac gaattttgtt
    33421 gaaggtgaac tctttctctt tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc
    33481 tttacccagc aatgccatat cgcgcgcggt actgaactgc cccggcgcat ccagaccgtg
    33541 caccgtctgg aaggtagtgt tggtcagacc cagttttttg gcataaccat tcatcagacc
    33601 aataaatgac tcctggctcc cggcaacgta atcagccagc gcaatacagg cgtcattacc
    33661 ggactggata atcacacctt tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag
    33721 gaacattacc gatgaaccac gcagtgccgg atttcccgtc gcccaggcat ctttaccgac
    33781 cgtcaccata tcggtgagtt taatcttatc ggccttaagc gcctgcccaa ccacatagct
    33841 ggtcatgatt ttagtcaggc tcgcggggtc cagtttttca tccgcattgc cttctgccag
    33901 cactttaccg ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc
    33961 aacggtttgt tccgccgcga atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc
    34021 tgcaagacca cgaaggagag aggagtattg cgtcataaat gccacccgag tatccattca
    34081 aaaaaaatac gttatatcgc cgtatgataa gttcggtgat taataacgca cacacaaact
    34141 taaagaaacc gtaagttggt aaagttttta aagtttatgc aatatcctgc cgcttcgctg
    34201 cttctggtgt catttttttg ccagctgttg cttaaaagtt aactttactt caccatcgga
    34261 gtcctgttca gcctgtggct gcctgtcgga aaaggggtga gtatgattac gctgtggggt
    34321 cggaataatt caaccaacgt aaaaaaagta ttgctgacgc tcgaagaact ggaactacct
    34381 tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc acgatgctga ttttctggcg
    34441 atgaacccta acgggctggt gccgttgttg cgtgacgacg aaagtgatct cattctttgg
    34501 gaatcaaacg ccattgtccg ctatctggcg gcacagtacg ggcaaaaacg cctgtggatc
    34561 gactcaccgg cacgtcgtgc ggaagccgaa aaatggatgg actgggcaaa ccagacgctc
    34621 agtaatgctc atcgcgggat cctgatggga ttagtcagaa caccaccgga agagcgcgat
    34681 caggccgcca ttgatgccag ttgcaaagag tgcgacgccc tgtttgccct gctcgatgcg
    34741 gaactggcaa aagtaaaatg gttctcaggc gacgagtttg gtgtgggcga tatcgctatc
    34801 gcaccgttca tctacaattt gtttaacgtt ggcctgacct ggacaccgcg tccaaatctg
    34861 caacgctggt atcagcaact cactgaacgc cccgcagtgc gcaaagtggt gatgattccc
    34921 gttagctaat tgcgtgggct aactttaagt aattccccac tggactcgtc ggtgagaacg
    34981 tataaataac cgtcgggtcc agtgcgaaca tcacgaattc gctgccctct gtccgttaaa
    35041 atacggccat cttctgtcac tttgtcgccg ttgacgctca tcacaatgac atctttatct
    35101 ttcagcgcgc caataaataa tttttgctgc cactggggga atttgtcgct gttatagaag
    35161 gccatgccgc tcacggcggg cgaatctttc cagtaaaaaa caggttgctc ggtcccggcg
    35221 acgatctccc ctttcgcctc cggtatctta aagcctgaat agttgattcc ccaggttgcc
    35281 agcggccagc cgtagttttt gcctttttgc gggatattaa tttcatcgcc accgcgcggg
    35341 ccatgttcat tcagccatag tgcattactc cacggattca tcgccattcc ttgcggatta
    35401 cgaatgccat aagaccagat ctcggcgcgt gcatcggatt cctttataaa aggattatca
    35461 tccgggattt ctcccaggtc ggtcagtcgc accagtttgc cctgtaattt atccagatcc
    35521 tgcgccgtcg ggcgctgatt gttttcgccc agagcaataa aaagataacc tttaccgtca
    35581 aataccatcc gcccgccaaa atggttgccg gtagacagtt ttggcatctg acgaaagacg
    35641 gtgcggaagt cggtcacttt tgagagatca tcacttaagc ggccataacc cacagcagtt
    35701 ccggctttgc catcatcgcc aacttcggaa taacttaacc agatgcggcg cgactgagca
    35761 aaatcgggcg ctaaaaccac gtccagcagg ccgccctgcc cgtgcgccca aacgtccggc
    35821 actccggaaa gcggcgcaga taatcctttt cctgcttgcc agtgacgcaa ctcgccgccg
    35881 cgcagagtga ttaacatacc gtgattgtcg ggtaaaaagg ccagcgccca gggatggtcg
    35941 agtttgtcct gcagtacttc gacatttacc gttgcaggag ccgcccagag agcggaagaa
    36001 agacaaataa ggggcacaag gaaaaaggat tgtcgatgca taacgcctcc ggattgactc
    36061 tggcttaaag cgtagtcagt ggaggagata acaaatttat ttttacaaaa acttaaacat
    36121 gaagggggag acgctttctc ccccttagtt ttcaggcctt ctcaagcatg gcgtgcttct
    36181 gcaggctctg gatactcagc gttaagctca tcagacaatt ttcaagctta tcggcgttga
    36241 cggtaataac agtcgggcaa tcatggtgcc cactcatcaa acatactgcg gctgtcgcta
    36301 atgcttcttc agcatgatga agagcactcc actcttcctg atccagatga agattcaacc
    36361 gcagcgattt atcgtgcagt tcgcgattca gtttaaaaaa gttatctcgt agatgattgc
    36421 tttcgctgac ggacatgtat ccttttgcct tcctcagttg cacgtatgcg gcgaggtcaa
    36481 cgcgtgcgtc gatcaaccgg ttcagcagat cgattcgctg tctgtcaacg aacatacttc
    36541 gatcctcctc tttccagcct tgttgcaatt aaatagtatg gctatttttt gagcaaaatg
    36601 ctgtctagga tcaattcttc acgccgaatt aacaaaattt acatttcctt cgcctccgcc
    36661 agcgaatgag attgcgatac aaattttatc cagaactctg tagaatccct gccctgacct
    36721 ttactgtaac tgaatatttt ttaagctatg agcaaagtaa ctccccagcc gaaaatcggc
    36781 tttgtttccc ttggctgccc gaaaaacctt gtcgattcag agcgtattct caccgaacta
    36841 cgcactgaag gttatgacgt ggtaccgagc tatgacgatg cggacatggt gatcgtcaac
    36901 acctgcggct ttattgacag cgcggtacaa gaatcactgg aagccattgg tgaagcgttg
    36961 aatgaaaacg gcaaggtaat tgtgaccggt tgtctggggg caaaagaaga tcagatccgc
    37021 gaagtccacc cgaaagtgct ggaaatcacc gggcctcata gctatgagca ggttctggag
    37081 cacgttcatc actacgtgcc aaaaccgaaa cacaacccat tcctgagcct ggtgccagaa
    37141 caaggtgtga aactgacgcc gcgtcattat gcctatctga aaatttctga aggctgtaat
    37201 caccgctgca ccttctgcat tattccgtct atgcgcggcg acctggtgag ccgtccgatt
    37261 ggcgaagtgt taagtgaagc gaaacgtctg gttgatgcgg gcgttaaaga gattctggtg
    37321 atctcgcagg atacttccgc ctatggcgtc gatgttaaac atcgtactgg cttccacaac
    37381 ggcgagccgg taaaaaccag catggtcagc ctgtgcgaac agttatcgaa actggggatc
    37441 tggacacgtc tgcactacgt ttacccttat ccgcatgtgg acgacgtcat cccactgatg
    37501 gcagaaggca aaatcctgcc ctatctggac attccgttgc agcacgccag cccgcgcatt
    37561 ctcaaactga tgaagcgtcc gggttctgta gatcgccaac tcgcgcgcat caaacagtgg
    37621 cgcgaaatct gcccggaact gaccctacgc tcaaccttta ttgtcggctt ccctggcgag
    37681 acggaagaag atttccagat gctactcgac ttcctgaaag aagcacgtct ggatcgcgtt
    37741 ggctgcttta aatacagccc ggttgaaggt gcagacgcca atgccctgcc tgaccaggtt
    37801 ccggaagaag tgaaagaaga acgctggaac cgtttcatgc agttgcagca gcagatttcc
    37861 gccgagcgcc tgcaagagaa agtgggccgt gaaattctgg tgattatcga cgaagtggac
    37921 gaagaaggtg cgattggtcg cagcatggca gatgcaccgg aaatcgacgg cgcggtttat
    37981 ctcaatggtg aaaccaacgt taagccgggt gatatcctgc gtgtgaaagt cgagcacgcc
    38041 gatgagtacg atttgtgggg tagccgggtt taattaatct taaggcagat ctaacaagga
    38101 tattgttctt cgatctgcct ctgctaccct tcccgatgtc ctacccttac gcacaaaatc
    38161 ccctgacgca taaatcgaga aaaatgaatc gtactcgagg caactctcac tttttcacac
    38221 caaattaaag ttttgatcgc aattacttga aataagcgta atggttacta tagtaaacat
    38281 taccacaagg atggatgtat gggtgtgtat aaggcaagac gattcagcca aagcacgaaa
    38341 aaacttggta ttcacgataa agttctcatg gctgccgctg aagaagtgat tcagggcata
    38401 tgggaggctg atttaggtag cggtgtcatc aaaaaacgtt tacccctcca acaaggaaag
    38461 agtggtggag ccagaacaat tatctttttt aaatcagcaa atcatgtgtt tttttatgat
    38521 ggttggtcca agagtggtct aagcagtaaa ggaaccaaag aaatcgaaga tgatgaactg
    38581 gcggcctaca aaaaaatggc aaatgcattt ctggccttta gcaataagca aatagaggat
    38641 ttgatcgaaa ctggcttttt gattgaggtg aaaaatgagc gataaattac aaatgatgct
    38701 ggaagatgca aaggaactga atgcactggg cctgatgtct gatagtgaag ttgatgccat
    38761 tgccgtcatg gtcaaagctc gtgaagtcag cgcccgtata gccaaagtca aatcaatgac
    38821 gggtgaagag atcaaagcca tccgcacccg atacggcatg tcacaatccg tgctggcaca
    38881 tacaatggga atgtcaaaag aaagtgtctc gaaatgggaa cgtaatgaaa taaaaccgag
    38941 cggcccggca ttacgtattt tgaatacatt ggcaataaaa gggccggaag tttttgcaaa
    39001 gtaaaatgtt tgcaaatgat ttaccacatt cattagtacc ccttgattaa ggggtactaa
    39061 cactattcta attctactgc cagctaatct gcgccctgcc gtttttctta ctcttataca
    39121 gtcgctcatc cgccagcatt ttcaagcgtt cgacggtagt acattcattt gaatgcgcta
    39181 caccgatact ggtgcttaaa ttaatgcttc ccacctggaa atgaacaatt tcttttagaa
    39241 tcgatagcaa ctcttccagc gttccacccg tataaatcac cgcaaattcg tcaccgccga
    39301 agcggtagac tttaccttta tctacgggga gctgtgattt aaggattttc accactttca
    39361 tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt aatgtttttg aatttatcga
    39421 cgtcaaacag gcacaaataa ccgccttttt ccggcagcgc ttttaaatct tcgtcaaagg
    39481 attttctgcc catcgctttg gtcagatcgt ccaggaatgc cgagttatac atactgtaga
    39541 gttttttgac atcggcagcc gaaaagaaaa gcccgccatc cagtacgcgt tcaaaatggt
    39601 tattaataat ggcttctttc tccacaatac cacgattaat cactctgcgt accagataga
    39661 ggcaactggc acccgatatc actgccacca atgcggcaaa gaaaataatc aatgtacata
    39721 cctgaaaata aacgtctttt ttataagcaa atacgtacaa cccggtcggc aaataggcga
    39781 agtgctccat cgcttccgaa acagtgaaga tggcgttttt agcgttatat tcatcaccgg
    39841 tcaccgattt accgttcttg aacagggaga acatcaggtt ctcgctatcc atcgccagcg
    39901 attttgacag taaagaaaaa cgttccagac caagcaaaaa tatcagacta cgattgtgag
    39961 ttaccggata gaggtaatag acgaaggttt taccatcaaa agagataaac cctgtatttt
    40021 tgacatgtcg atacttcaca acctgcataa tatctgcaca ataatttttc tcgaactcct
    40081 ttacctgaat acattcgtca ctggaaatca gataaggacc gacatcgtac aaataagagg
    40141 gtgaaaccga gtcggcgtaa gctgtgcgta agccaaccaa cagctgttcc tccgtgggat
    40201 ttagcgtgtt gataagatca attgccacgc tacgccgata ttcagcaata tttgccaccg
    40261 caaaagcagt atcaataacg gactgtttca ggctatacac ccgttccttt accatttgag
    40321 tgtagatccc gcaggcaact gctgaaataa tgataaaaat cagcagactg actgtaagta
    40381 cgaacttatt gattctggac atacgtaaac tactctttta ctaattttcc acttttatcc
    40441 caggcggaga atggcatggg tttctggtac agatacccct gagcctgaac acatcctaat
    40501 ttgattaatt cgcgcgcaac gtcttcgctc tctacgcctt ccgcaatcac cgggatccgg
    40561 ttatagcgtg ccagatcgaa aataatgcgc acaatttttc ccgcagtacc aaaaatatcg
    40621 ccggtaaact gcgagtccac ctttatgctg tctggattaa tttcataaaa acgtttcagg
    40681 tttgacaaac cggtgccaaa gtcatcaatc gatatccgta ccccttgttt acgcagttgt
    40741 tcaatattga gataaaaatg ttcattaatt cgctcaatca cggtttcagt gatctcaaca
    40801 caaaggctgt tcgccttcag gccattttct ttcatgatgg tagtaaaacg ctcatgaaag
    40861 tgtggttcgt ttaactgcca gggcgaaaca ttaatatgca gtaagaaatc atccttcagc
    40921 gacgcacgct gactaaaatg tctgaattca cccatcgcgg ttttaataat ttgatagccc
    40981 agctcattga taaacccgat atcttcagca atggtaatga acttcagcgg tgaaatgatc
    41041 ccccgctccg cagacaccca acggcacaac gcctccgcgc ccagaatggc gcgggtgtta
    41101 atatcaacga tcggttgata gacgaggaat agttcgccaa tttccattgc atggcgaata
    41161 ttacgcgcaa tccagatatt ctcttctatt tcgttgagtt tcgcatcatc acagaaaata
    41221 aactctgaga acatatggtt gcggatactg gaaatcgcca gtttcagctt ttctgcgtaa
    41281 ctccaggcgt tttccccctg caaaggttca caaatagcag acttatggat gcgcagattg
    41341 cggtagccaa aactgctgtc aaaatcgttt accgtcgcta tcttacgggt aaacgtctgt
    41401 acattttccc ggcacaggag taaatagaga tcggcattaa atgtcacgat atcgcagcat
    41461 tgcgcatatt tctcggcaat tcgctgacac aatttcctga cgattttatt gcgttcatca
    41521 acaccaaaca ccgcttccac agcttccaga ccacacagtt agatgatccc cacagccaga
    41581 tactgattat tgttggattc aataatcgcc atatttttaa taaaaccgct ttcctgatcg
    41641 taagagatca gtgagagcaa tttatttgac cgttcaattt cggcatcttt catttccgcg
    41701 atcgaatttt tgatgctgat aatctctttc gcatcgcctt cgcgaatttt cagagcctcg
    41761 atctgatccg ttgagtcggg caaaaacgcc agcgtattaa ccagctcggt catttcagaa
    41821 aattgccgac ggaacacacg agtggtcatt aaataaacca ccaacaatac gataataatg
    41881 aacgcaaaca gcagataaaa aagt
//
LOCUS       DAZKJR010000024        41668 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKJR010000024 DAZKJR010000000
VERSION     DAZKJR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.024"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(267..533)
                     /locus_tag="ACHOWS_001343"
                     /pseudo
     CDS             complement(267..533)
                     /locus_tag="ACHOWS_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(941..2146)
                     /locus_tag="ACHOWS_001344"
     CDS             complement(941..2146)
                     /locus_tag="ACHOWS_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0046956.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFKKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFKTKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIETQLAHVDKNAIRGTYNHALYLDGRREMMQWYADYIDSLSSH
                     ESQCQGPLY"
     gene            complement(2446..2540)
                     /locus_tag="ACHOWS_001345"
     tRNA            complement(2446..2540)
                     /locus_tag="ACHOWS_001345"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2504..2506),aa:Sec,seq:tca)
     gene            2833..4215
                     /gene="yicJ"
                     /locus_tag="ACHOWS_001346"
     CDS             2833..4215
                     /gene="yicJ"
                     /locus_tag="ACHOWS_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFQ0046957.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            4225..6543
                     /gene="yicI"
                     /locus_tag="ACHOWS_001347"
     CDS             4225..6543
                     /gene="yicI"
                     /locus_tag="ACHOWS_001347"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFQ0046958.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKHPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEMPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLMVTPQGNALTITL"
     gene            6839..8392
                     /locus_tag="ACHOWS_001348"
     CDS             6839..8392
                     /locus_tag="ACHOWS_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0046959.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENITDLLHLDHLLVAECEEIDGVMNNSRRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            8419..8889
                     /locus_tag="ACHOWS_001349"
     CDS             8419..8889
                     /locus_tag="ACHOWS_001349"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0046960.1"
                     /translation="MDITKILNTNRVILDMQATNKEEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            8907..9221
                     /locus_tag="ACHOWS_001350"
     CDS             8907..9221
                     /locus_tag="ACHOWS_001350"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0046961.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            9246..10337
                     /locus_tag="ACHOWS_001351"
     CDS             9246..10337
                     /locus_tag="ACHOWS_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0046962.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            10349..11200
                     /locus_tag="ACHOWS_001352"
     CDS             10349..11200
                     /locus_tag="ACHOWS_001352"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0046963.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVAVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            11281..12141
                     /locus_tag="ACHOWS_001353"
     CDS             11281..12141
                     /locus_tag="ACHOWS_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0046964.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            12189..13121
                     /locus_tag="ACHOWS_001354"
     CDS             12189..13121
                     /locus_tag="ACHOWS_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0046965.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            13102..13782
                     /locus_tag="ACHOWS_001355"
     CDS             13102..13782
                     /locus_tag="ACHOWS_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFQ0046966.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(13826..15535)
                     /gene="yicH"
                     /locus_tag="ACHOWS_001356"
     CDS             complement(13826..15535)
                     /gene="yicH"
                     /locus_tag="ACHOWS_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFQ0046967.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGSWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVTGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(15656..17047)
                     /gene="xanP"
                     /locus_tag="ACHOWS_001357"
     CDS             complement(15656..17047)
                     /gene="xanP"
                     /locus_tag="ACHOWS_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFQ0046968.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            17326..18531
                     /gene="gltS"
                     /locus_tag="ACHOWS_001358"
     CDS             17326..18531
                     /gene="gltS"
                     /locus_tag="ACHOWS_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFQ0046969.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(18565..20646)
                     /gene="recG"
                     /locus_tag="ACHOWS_001359"
     CDS             complement(18565..20646)
                     /gene="recG"
                     /locus_tag="ACHOWS_001359"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0046970.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(20652..21341)
                     /gene="trmH"
                     /locus_tag="ACHOWS_001360"
     CDS             complement(20652..21341)
                     /gene="trmH"
                     /locus_tag="ACHOWS_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFQ0046971.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(21348..23456)
                     /gene="spoT"
                     /locus_tag="ACHOWS_001361"
     CDS             complement(21348..23456)
                     /gene="spoT"
                     /locus_tag="ACHOWS_001361"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0046972.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(23475..23750)
                     /gene="rpoZ"
                     /locus_tag="ACHOWS_001362"
     CDS             complement(23475..23750)
                     /gene="rpoZ"
                     /locus_tag="ACHOWS_001362"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0046973.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(23805..24428)
                     /gene="gmk"
                     /locus_tag="ACHOWS_001363"
     CDS             complement(23805..24428)
                     /gene="gmk"
                     /locus_tag="ACHOWS_001363"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0046974.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            24686..26371
                     /gene="ligB"
                     /locus_tag="ACHOWS_001364"
     CDS             24686..26371
                     /gene="ligB"
                     /locus_tag="ACHOWS_001364"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFQ0046975.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVIHPVAHTGVR
                     KMADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPQTVSGPLANSTLQGEIFLKREGHIQKQMGGINARAKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKTKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVARVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRSLHQTHRFEHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLLSTEQFWQQLPGTGSGRVRQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(26368..26985)
                     /gene="yicG"
                     /locus_tag="ACHOWS_001365"
     CDS             complement(26368..26985)
                     /gene="yicG"
                     /locus_tag="ACHOWS_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFQ0046976.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(27277..28101)
                     /gene="dinD"
                     /locus_tag="ACHOWS_001366"
     CDS             complement(27277..28101)
                     /gene="dinD"
                     /locus_tag="ACHOWS_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFQ0046977.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVNIKQLENSVKITEKK"
     gene            complement(28323..29186)
                     /gene="yicC"
                     /locus_tag="ACHOWS_001367"
     CDS             complement(28323..29186)
                     /gene="yicC"
                     /locus_tag="ACHOWS_001367"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0046978.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            29313..30029
                     /gene="rph"
                     /locus_tag="ACHOWS_001368"
     CDS             29313..30029
                     /gene="rph"
                     /locus_tag="ACHOWS_001368"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0046979.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            30095..30736
                     /gene="pyrE"
                     /locus_tag="ACHOWS_001369"
     CDS             30095..30736
                     /gene="pyrE"
                     /locus_tag="ACHOWS_001369"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0046980.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(30773..31369)
                     /gene="slmA"
                     /locus_tag="ACHOWS_001370"
     CDS             complement(30773..31369)
                     /gene="slmA"
                     /locus_tag="ACHOWS_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFQ0046981.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(31476..31934)
                     /gene="dut"
                     /locus_tag="ACHOWS_001371"
     CDS             complement(31476..31934)
                     /gene="dut"
                     /locus_tag="ACHOWS_001371"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0046982.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(31912..33132)
                     /gene="coaBC"
                     /locus_tag="ACHOWS_001372"
     CDS             complement(31912..33132)
                     /gene="coaBC"
                     /locus_tag="ACHOWS_001372"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFQ0046983.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            33304..33972
                     /gene="radC"
                     /locus_tag="ACHOWS_001373"
     CDS             33304..33972
                     /gene="radC"
                     /locus_tag="ACHOWS_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0046984.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            34189..34425
                     /gene="rpmB"
                     /locus_tag="ACHOWS_001374"
     CDS             34189..34425
                     /gene="rpmB"
                     /locus_tag="ACHOWS_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0046985.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            34446..34613
                     /gene="rpmG"
                     /locus_tag="ACHOWS_001375"
     CDS             34446..34613
                     /gene="rpmG"
                     /locus_tag="ACHOWS_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0046986.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            34711..35520
                     /gene="mutM"
                     /locus_tag="ACHOWS_001376"
     CDS             34711..35520
                     /gene="mutM"
                     /locus_tag="ACHOWS_001376"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFQ0046987.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGESCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(35559..36038)
                     /gene="coaD"
                     /locus_tag="ACHOWS_001377"
     CDS             complement(35559..36038)
                     /gene="coaD"
                     /locus_tag="ACHOWS_001377"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0046988.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(36046..37323)
                     /gene="waaA"
                     /locus_tag="ACHOWS_001378"
     CDS             complement(36046..37323)
                     /gene="waaA"
                     /locus_tag="ACHOWS_001378"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFQ0046989.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            37736..38794
                     /gene="rfaQ"
                     /locus_tag="ACHOWS_001379"
     CDS             37736..38794
                     /gene="rfaQ"
                     /locus_tag="ACHOWS_001379"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFQ0046990.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            38791..39915
                     /gene="waaG"
                     /locus_tag="ACHOWS_001380"
     CDS             38791..39915
                     /gene="waaG"
                     /locus_tag="ACHOWS_001380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0046991.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQFSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            39908..40705
                     /gene="rfaP"
                     /locus_tag="ACHOWS_001381"
     CDS             39908..40705
                     /gene="rfaP"
                     /locus_tag="ACHOWS_001381"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFQ0046992.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKKEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVASLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            40721..>41668
                     /gene="waaO"
                     /locus_tag="ACHOWS_001382"
                     /gene_synonym="rfaI"
     CDS             40721..>41668
                     /gene="waaO"
                     /locus_tag="ACHOWS_001382"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFQ0046993.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKLIEAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNF"
BASE COUNT        10108 a        10006 c        10941 g        10613 t
ORIGIN      
        1 ataaagcgaa acaaatattt ttttacttat tttaattagt agaggttcgt atgagtaaaa
       61 ataactttag taaaagtccc tattatagaa gccgtagcaa aacgaaattt taacagaagc
      121 aacttcattt tgtaagtgtc cggcttaata atgaagagct cacgcttcta aacgataaac
      181 gtggtagcca tcggcttcct ttattttttc ttaatgtgct tcctagtatt gttccagtca
      241 ttaatataga agcatgggat ctttagcagg cttttcacag aaataaataa aaatattgcc
      301 caccttggct gctccagaaa tgcatatgcc agatgagcgt gtatatcaat aaagtcaggc
      361 tcccgttggg caagctacct gagtttgttg atataactcg tctcgctgat ttcatcagtg
      421 ccgtatgcgt catgcacttc ttcaaactcc tccagtaatg ggccaaacct ggtattcgga
      481 aatacgaaac ggccttggtt atcgttcaca tcaaagcgta atctgtcgtt cattttctga
      541 gcctgtaaaa cggatgggac acaatctgaa aagcataaaa gtcgtgatgt tgtgtatcaa
      601 gtcttgttat tgcccgggat gaatattgtt tacaatatca tcgacagtaa ttcttcatgt
      661 ggataaaaat ttatctttat ctgcattcca atgcattgtg aatagtcggc taagacaaca
      721 tgttagttga tttttggtgt gatggtgctg aaataacgac aggatgatga ttgaaattac
      781 aggcatcaaa attgttagcg gtctatagcg gtgtgatgtt ctgagattgt ttgcagatgc
      841 aatataaata ttttgtcaaa aaatatctaa aaataaaaca tgaaaacaaa attcctccag
      901 acctggaggc cggaggaatt ttgtgaattg agagaaaata tcagtacaag gggccttgac
      961 actgactctc gtggctggaa agcgaatcaa tgtaatcagc gtaccattgc atcatttcac
     1021 ggcgaccatc tagatacagg gcatgattat aggtccccct gatagcgttt ttatctacat
     1081 gagctaactg cgtttcaatc caagcggaat taaagccctg ttcgtgtaga atggtactca
     1141 tggtgtgtct gaaaccgtgc cctgtgagtc ttcccttata tcccagtaat tcaatgactt
     1201 ggttaatgct ttccttacta attggcttgc gaggatcgtt cctaccgata aagaccagag
     1261 gatgatgttt tgtgatgggt tcaagttgct taaatagggc aatgacctgc tcagagagcg
     1321 gcacaatatg aggtcgcttc atcttcatta cttctgccgg aatctcccat aattttgtct
     1381 taaaatcgat atcctcccag cgtgcaaaac gtaattcttg tgttcgtaca ccggttaaca
     1441 taatgatctg agtagctgtt ttagtgatga tacttccggt ataacccgcc agatcgttga
     1501 ggaagtgggg aagttcatta gcagtaagaa agggaaaatg tacttttttc ggtgtagcta
     1561 aagcacaggc aagatcggga gcagggttgt agtctgcccg accagtaaca attgcatagc
     1621 ggaacacctc accacagcgc tggcggactt tgcgcatttt ctccagagct ccacgttttt
     1681 ccattttacg cagtgcttcc agtaactcca ttggtttgat ttcggcgata gggcgtttgc
     1741 caatgtaggg aaaaatgtct tttttaaagg tatcaatgat ttcatcacga tagcgtaatg
     1801 accaccgatc ggcttttgac tgatgccatt cgcgagcaat ggcctcgaac gtatttttca
     1861 aactcatttg ctgcgcgatt ttttcttctt tttttgccac ccctggatcg cctccagcag
     1921 cgaggatttt tctggcttcg ctgcgttttt cacgagcgtc ggctagcgtt acatcggggt
     1981 atacgccaat agaaagcatc ttttctttac cagcaaagcg gtacttcata cgccagtatt
     2041 tagaaccatt agtgttaatc agtagataca taccgccgcc atcagcaagt ttatagggct
     2101 tttcttttgg ttttgcggtt ttcactttaa tatcggttaa tgccatgaca tcctccggct
     2161 tttaatgaat tcatgttcct gagggggatt tctgccagta attaacctga tacccccaat
     2221 gcaccccttg tataacaaag atgttgttga aagccgttgt tatatatcat attttaccaa
     2281 ttatgggggt gctgatattt caataggaaa taataccccc taaagtaccc ccaaaagcaa
     2341 cttgatacgg ggtgaagtcg gttgacttct ggatacaaag gaatcctgat atacaaggga
     2401 ttagaagggg gggattgaca tcgggagagt tcagaagatg ttaattggcg gaagatcaca
     2461 ggagtcgaac ctgcccggga ccgctggcgg ccccaactgg atttgaagtc cagccgcctc
     2521 accggagacg acgatcttcc gcgcctcgat tgctacatgg aggcggggcg cattatagct
     2581 acttccttga gtttctacat cccccagatc agttttttcc gcctaataca cgcagcgtat
     2641 tatcacgaaa acattatagt tcatataaaa ctcatatttt acaatgatat atgagcgaaa
     2701 ttaacgctac cacgatcaca taaaacaaga atcataaatt aataaccaga tatcggaata
     2761 ttcgctctcg cagggatggt ttacaaaatg cgttatcggt ctcagcaccc ctatagcatc
     2821 aaggaaaagc agatgaagag tgaagtgttg tccgttaaag agaaaattgg ttatggcatg
     2881 ggagacgccg ccagccacat tattttcgat aacgtaatgt tatatatgat gttcttttat
     2941 accgatattt ttggcattcc tgccggattt gtcggaacca tgtttttggt cgctcgtgca
     3001 ctggatgcga tttccgatcc ttgcatgggg ttgttggccg atcgaacgcg ctctcgctgg
     3061 ggtaaatttc gtccgtgggt actgtttggc gcactgccat ttgggatcgt ctgtgtactg
     3121 gcctatagca cgccagatct cagtatgaac ggaaaaatga tttatgcagc aattacttac
     3181 accctactta ccttacttta taccatcgtt aatatccctt actgcgcatt gggtggtgta
     3241 atcaccaatg acccgactca gcgtatctcg ctgcaatcct ggcgttttgt gctggcgacc
     3301 gcgggaggca tgctctctac tgtgctgatg atgccgctgg ttaatttaat tggtggtgat
     3361 aataaaccac tcggtttcca gggcggtatc gcggtccttt ccgtggtggc attcatgatg
     3421 ctggcattct gtttcttcac cactaaagaa cgcgttgaag caccacctac aacgacgtct
     3481 atgcgggaag atttacgtga tatctggcaa aacgaccagt ggcggattgt cggtttacta
     3541 accattttca atattctggc ggtgtgcgta cgcggtggag cgatgatgta ttacgtcaca
     3601 tggattttgg gtacgccgga agtgtttgtc gcttttctca ccacttattg cgtgggtaac
     3661 ctgattggtt ccgcactggc aaaaccgctg accgactgga aatgtaaagt cactatcttc
     3721 tggtggacga acgccctgct ggcagtgatt agcctcgcga tgttctttgt tcccatgcag
     3781 gccagcatca ctatgtttgt cttcatcttc gtgattggtg tattgcatca actggtgaca
     3841 cctatccagt gggtaatgat gtccgatacc gtcgactacg gcgagtggtg caatggtaaa
     3901 cgcctgaccg ggatcagttt tgctggcacg ctgtttgtgc tcaagctggg gttggccttc
     3961 ggcggcgctc ttatcggctg gatgctggct tatggcggat atgatgcggc agaaaaagcg
     4021 cagaacagcg ccacgattag catcattatc gcgctattca cgattgttcc ggcgatctgt
     4081 tacttgctga gcgcgattat cgctaaacgc tactactcac tcacgacgca caatctgaaa
     4141 accgttatgg aacagctggc gcagggcaaa cgccgttgcc agcaacaatt cacctctcaa
     4201 gaagtgcaga actaaggaac ggcaatgaaa attagcgacg gaaactggtt gattcaacct
     4261 ggcctcaatt tgattcaccc gcttcaggtg ttcgaggttg aacagcaggg taatgaaatg
     4321 gtggtctatg ctgccccccg tgatgtgcgt gaacgtacct ggcagcttga tacaccttta
     4381 tttacgctgc gctttttctc cccacaggaa ggtattgttg gtgtacggat tgagcatttt
     4441 cagggggcgc tgaataacgg tcctcattat ccgctcaata ttttgcagga tgtgaaggtc
     4501 acaatcgaaa acacagaacg ttatgctgag tttaaaagtg gcaacttaag cgcgcgtgtc
     4561 agcaaaggtg agttctggtc actggatttt ctgcgcaacg gcgaacgtat taccggtagt
     4621 caggtgaaaa ataatggcta cgtgcaggac acgaataatc aacgaaatta tatgtttgag
     4681 cggctggatc ttggcgttgg cgaaacagtt tacggtctgg gagagcgctt tactgccctg
     4741 gtgcgcaatg gccagacggt agagacctgg aaccgggacg gcggcacaag tactgaacag
     4801 gcgtataaaa atatcccgtt ctatatgact aaccgtggtt atggggtact ggtcaatcat
     4861 cctcaatgcg tctcttttga agtgggatcg gagaaagtct ccaaagtgca gttcagcgtt
     4921 gagagtgaat atctcgaata ctttgttatc gacggcccga cgccgaaagc ggtacttgat
     4981 cgttataccc gttttactgg tcgtccggcg ctgccgcccg cgtggtcctt cggcctgtgg
     5041 ctaaccactt catttaccac caactacgac gaagcgacgg taaacagctt tatcgatggt
     5101 atggcggaac gcaatctgcc gctgcatgtt ttccactttg actgtttctg gatgaaagcc
     5161 ttccagtggt gcgattttga gtgggacccg ctgactttcc cggacccgga agggatgatc
     5221 cgccgcctga aagcgaaagg gctaaaaatc tgcgtctgga ttaaccccta tattgggcaa
     5281 aaatcccctg tctttaaaga gttacaagag aaaggctatt tactcaaaca cccggacggt
     5341 tcgttatggc agtgggataa atggcagcca ggtctggcaa tttatgactt taccaatccg
     5401 gatgcctgca aatggtacgc cgacaaactg aaaggtctgg tcgcgatggg cgttgattgc
     5461 tttaagaccg actttggcga acgtattcca accgatgttc aatggtttga cggttccgat
     5521 ccgcagaaaa tgcataacca ttatgcgtac atctacaacg aactggtgtg gaacgtgctc
     5581 aaggacaccg ttggtgagga agaagctgtc ttgtttgccc gctcggcttc cgtcggtgcg
     5641 cagaaattcc cggtacactg gggtggcgac tgttacgcta actacgaatc aatggcggaa
     5701 agcctgcgcg gtggtttgtc tattggcctt tcaggttttg gcttctggag ccacgatatc
     5761 ggcggctttg agaataccgc gccggcgcat gtttacaaac gctggtgcgc gtttggtttg
     5821 ctctccagcc atagccgttt acatggcagc aaatcttatc gtgtgccgtg ggcctacgat
     5881 gatgagtcct gtgatgtggt gcgcttcttc acgcaactga aatgccgcat gatgccgtat
     5941 ctgtatcgtg aagctgctcg tgcgaacgcg cggggtacgc cgatgatgcg ggccatgatg
     6001 atggagtttc cggacgatcc ggcttgtgat taccttgacc gtcaatacat gttaggcgac
     6061 aacgtgatgg ttgcgcctgt gttcactgaa gcgggcgatg tgcagttcta cttgcctgaa
     6121 ggtcgctgga cacacctgtg gcacaacgat gaactcgatg gtagtcgctg gcataaacag
     6181 cagcacagct tcctgagtct gcccgtttat gtgcgtgata acaccctact ggcgctgggc
     6241 aacaacgagc aacgtcccga ttacgcgtgg cacgaaggca cggcattcca gctctttaat
     6301 ctacaagacg ggcatgaagc cgtctgtgaa atgcccgctg ctgacggttc ggtgatcttt
     6361 actctaaaag caacacgtac tggtaacacg attactgtga ctggtgcggg cgaggcgaag
     6421 aactggacac tgtgcttgcg caatattgtg aaagtaaatg gtctgcaagg cggttcgcag
     6481 gctgaaagtg agcaggggct gatggtgacg cctcaaggga atgcgctgac aattacgttg
     6541 taatctattg atggaatgcc gggtacgaat ggttttgtgt cgtatccggt aaataatctc
     6601 acatctggta ataaatattt cttccatcgt atttgcgagc atgatcacga ttattcatcg
     6661 cgaaaatgtg atctgtgctg aaaaatatcc cttaaccgcc ttgctttgtt ttatcactgt
     6721 ccggattacg gacagatttt agtattgttg tgaaattatt ctgctttata tttaccctca
     6781 gagatggcaa ctgaagctct tcctcatgag gcttcttaat ctgtgataag ggtaaaaaat
     6841 gcaaatgatt acctcgcgac aaaacagatt attacgattc cttttacctc gaagggaata
     6901 tacgactatt gttacgattg ccggctattt aaatgtttcg gaaaaaacca ttcaacgtga
     6961 tttacgttta cttgagcaat ggctgggaca atggagaata aatgttgaga agcgtgctgg
     7021 cgcgggtgtg atgttaagcg cggagaatat tactgatttg ctgcatcttg atcatttgtt
     7081 ggtggcagaa tgtgaagaga ttgatggtgt aatgaataat tccaggcgcg ttaaaatagc
     7141 gtcgcagtta ttaagtgaaa caccgaatga aacgtcgatc agtaaattgt cagaacgcta
     7201 ctttatcagc ggagcctcta ttgttaatga cctgagagta attgagtcct ggcttgcgcc
     7261 gttggggtta tcgttgatcc gcagtccaag tggtacgcat attgaaggta gtgaagggca
     7321 agtccgacag gcaatggcat tactgattaa cggcattatt aaccataatg agccgcaagg
     7381 tgtcgtgtat tcacgtctgg atcccggaag ctataaagca ttagtccatt attttggaga
     7441 agaagaggtg ttatttgtcc agtcattgtt actggatatg gaaaatgaat taagttggtc
     7501 tttgggagaa ccttattacg ttaacatttt tactcacatc cttattatga tgtatcgcaa
     7561 cacgcacggg aatgcgttat caagagaaga agatcaaacc aggcaatatg atgaaaatat
     7621 ctttaatgtt gccagtcaga tgattcataa gatagaacaa cgaattgcac atacattgcc
     7681 cgatgatgaa gtctggttta tttatcaata tatcatttca tcaggtgtgg cgattgatgg
     7741 acaaaaagat gttagcatta tttcacatat gcaggccagc aatgaagcgc gtctgattac
     7801 ctggcgttta attacggtat tcagtgacat cgtggactgc gattttagtg aagacagcgc
     7861 attatatgat ggcttattgg tgcatattaa accgctgatt aaccgactaa attatcgtat
     7921 tcatatccgt aatccattgt tggaagatat taaagcagaa ctagcggatg tctggcggtt
     7981 gacgcaatat gtggtgaatc aggtatttaa aacctggggt gagaatgcag tgagcgagga
     8041 tgaagtaggt tacctgaccg ttcattttca ggctgcgatg gagcggcaaa ttgcccgtaa
     8101 acgcgtatta ctggtctgtt caaccggaat cggaacttcg catctactga aaagccgtat
     8161 tctgcgagca tttcctgaat ggacgattgt tgatgttatt tcagcagcga atttatcaca
     8221 ggttttgcct gacaatatcg aactgattat ttcgaccatt aatttgccta cagtcactat
     8281 gccggtcgct tatgttaccg ctttttttaa tgatgccgat attaagcggg tcactgaaat
     8341 ggtgattacg gaaaaattac atcatgcgac atctcgggtc gttgaaattt aatgtcagaa
     8401 ttttatgagg tatgacgaat ggacataaca aaaatcctga atacgaatcg cgttatttta
     8461 gatatgcaag cgacgaataa agaggaggcg attgaagaat taactaatct tctcaaaaaa
     8521 gatggtgcga tcgattgtcg ggaaaccttt attaaagatg tctggcagcg cgaagcggaa
     8581 ggctcgactg gttttgaaaa tcatattgcg atcccgcatg gtaaatcttc agctgtgatt
     8641 aatactacgc tggcaattgg ccgtacccgg caggatattc cctgggaaac gctggatggc
     8701 agtagcgtgc gctgtattat tttatttgcg gtacgtcttg aagatcaaaa taccacccat
     8761 atccgtttac tttcccaggt cgcgggcgcg ctggcggatg atgacatcat agaacaattg
     8821 ttagttgaga cgagtccgca aaaaatcatt gatctcttta gtcagtatgc acaatccgat
     8881 gtctgctaaa taacagggag tatgtcatga atattgtctg tgttgccgcc tgtacggcgg
     8941 gtattgctca tacctatatt gctcgtgaaa agttaatcaa aggagcaaaa gctctgggtc
     9001 ataccattaa agttgaaacc cagggaacca ttggtacaga aaatgaatta caggcggcag
     9061 atatctctgc tgctgacgtc gtgattcttg ctgtagatgt gaaaataaaa ggagaagaac
     9121 gcttcaccaa taaacgtatt gtgcgagtaa agacagagat cgtcattaaa tcgcccgttc
     9181 aatttcttga aaaggtcgaa aaatcactgg gaaacaaatg atccttatac tggagggatc
     9241 ttaatatgaa agaaaataaa attccggttt cacaggaaat taaaaaacat ttattaaccg
     9301 gcatctcgtg gatgataccc cttattgtgg ccgccgggat atgtattgcg ttggggcaag
     9361 ttcttggtgg tacagatgtt ggtgaaaaaa caggaacaat cccgtggatg cttaatcaga
     9421 ttggcggctg gggaatgggg ctgattgtgc cgctgattag cgcggcaatt gcctattcta
     9481 ttgccgatcg tcccggattt gccccgggcc tgattgtcgg ctttctctgt gggcaaatcc
     9541 ataccggatt tattggcggt atgttgggtg gtttcctggt gggttacacc attttgctac
     9601 ttaaacgcta tatccgcctg ccgcagtcga tgcaaggact catgccaatc atggtgttgc
     9661 cggtattaag caccattatc ggtgggctgt taatgatgac gctgattggt aaacccatcg
     9721 cctggttaca agaagcactg attcatttgc tcgagtcgat gcagggcgga tcgcgcttcc
     9781 tgatgggggc catacttggc gcgatggcaa ccttcgattt cggtggtccg gtcaacaaaa
     9841 ccatgtcgct gttttccgat ggcttgctgg tcagtggcgt ctacggacca gaagccgtga
     9901 aatttgtcgg ctcaatcatc cctccgtttg gcatcacact ttcgttctta ctgacacgcc
     9961 ataaatacac ccgtgcggag cgcgaggcgc tgaaagccgc gtttccgatg gggatctgca
    10021 tgatcactga aggagtcatt ccgatcgccg cgcgtgacct gctgcgagta gtgggatctt
    10081 gtgtggtggc atcggcggtc gcgggcggct taatcatggt gtggggcgtg gagagtccgg
    10141 ttccgcatgg cggcatgttt gttgtgcctc tgttcaccca tcctctgctg ttttgcctgg
    10201 cgctgggtat aggcacggtg atttgtggcg tgatgctttc actctggaag aaaccggtaa
    10261 cggaacgcga cgaagagttt gacgaactga gcgatcaaaa gctgaaagat gaagagatca
    10321 ctttcaccct cgaataaccg gaggccctat gtttgccgat atgaaaagta tggtgatgaa
    10381 agcctggcat gaacattacg ctttgctggc gatcaactgt atgaatctgg aaagtgctca
    10441 cgcagctatc cgcgtggcgg agaagaatcg cgctccgatc attctcaatc tctatcaggg
    10501 gcatctggcg cattttcctg cacccgtcgc ggcggcagtg gttaaaacgc tcgcggaatc
    10561 tgcctcagta gcggtcgctc tggcgcttga tcatggcaaa aatccggact gtattcgtca
    10621 ggcgtttcgt gctggtttca gcggattaat gattgatgct tctgcttttc ccctggagga
    10681 aaacgtgcgc cagaccaggg cggtggtgga actcgccgcc agcgtgcagc tctgcgtgga
    10741 aggcgaactg ggccatctgg cagatgcgcc gcgttacgac caggccgcca atgcagacct
    10801 gatgacgcaa cccactgatg tggaaacgtt tatcgcgcag acaggcattg atctactggc
    10861 cgtctcggta ggcacggcgc acggcatgta cgcgcctggt gttgtacctg ctattgattt
    10921 tcagcgtctg gcggagattt cgcgctgctc gagcgtgcct ctggcattac atggcggtag
    10981 cggtacgccg ttcgatcagc tccagctctg tacgacgctg ggggtagcaa aaatcaacgt
    11041 gggggcggcc atttttgaac gggggaaatc cgcgctcctg cataccctct gtcacgatat
    11101 atccattgaa ctggtggacg cgctgaaagc tatggaactg gccttcgaag aagcgattac
    11161 accgtattta caggcgagcg gcgctatcgg caaagcctga ttcattttct gtcatggcct
    11221 ttatgccagt gcaataacgc accggcaggg cggactacac gctgaaactg gagatttaac
    11281 atgttagttt cgatgaaaga attactgcaa cctacccgcc agcacggctt tgccattggc
    11341 gcatttaatg tggcggataa ctgtttcctt cgcgcagtcg tggatgaagc agaagcgaca
    11401 aatactccga cgattattgc catccatccc agcgaacatg attttgtggg cgatgccttt
    11461 ttcgctcacg ttcgcgaaat tacccagcgt agtccggtcc cgtttgtact gcacctcgat
    11521 catggtgcct ctgtagagca tgttttgcgt gcgattcgct gcggatttac ctcagtaatg
    11581 atcgacggtt cgctgctgcc atatgaagaa aacgtggcgc tgaccgcaga agtggttcgc
    11641 ctcgctcacg cggttggagt atcggttgag ggtgaactgg gaaccatcgg acaaacagga
    11701 acgtcggtag aaggtggtgt ttcacaagtg acctacacag atccggcgca ggccgccgat
    11761 tttattgctc gtacaggtgc cgacacactg gctgtggcga ttggtacagc gcatggcatc
    11821 tatccaaaag ggatgcagcc gaagctgcaa atggacattt tgcgcgacat tgccggacgt
    11881 attgatattc cgctggtgct acacggtggt tcggcaaacc cggacgcgga gattgctgaa
    11941 tcagttacgc tcggcgtcgg caaaatcaat atctccagcg atatgaaata cgcctatttc
    12001 cagaaagtgc gtgaaattct cgctaaagag agctggtggg acccgaacgt catctatccg
    12061 gatgcgatta acgcagcgcg ggaagtcatt cgccacaaaa tgaaactgtt cggttcgctc
    12121 ggtaaagcat cactgtacta agccagaagg gcggggtgac ccgcctttta cgcaacaagg
    12181 aacgagatat gcactatctg gggttggata ttggtgggac caaaatcgcc gccgtcgtca
    12241 tggatgcgca tggctgggag attcgccgtt accgctgccc gacgcaaaag tcgacatatc
    12301 aacaatttgt ctcatgcgtt gtggcgctta tcgagcagat taggcgggac gttcaacgac
    12361 cgatgctgac agggatcgcc ttacccggca gtatctcgcc actcactggc ctgattaaaa
    12421 acgcgaatat tcaggtgatt aacggtcatg cgttacaggc tgatttgcag caattgcttg
    12481 ggcaaccggt ggtgatagcc aatgatggta actgtttcgc gctatcagaa gcttgcgacg
    12541 gtgccgggca agattatgac gtggtatttg gtattacgct tggtacgggc tgcggcggtg
    12601 gcattgccat caagcaacga ccgtttatag gggcctgggg aaatgctgcc gaatgcggtc
    12661 atatcacgtt gccaggctat acggagcagg aagatggtcc atcagtcagt tgctattgcg
    12721 gcaaacacaa ctgcgtggag tcgtttgttt ccggcagcgg ttttagtgaa cgctatcaac
    12781 agatgactgg taacttgctc actcctgcgg cgattgtcac cctggcacaa cgtggtgatg
    12841 cttgtgccat gcagcaggtg gcacgttttc gccaacagct tgcccgcacg ctggcaacca
    12901 tcgttaatgt tgttgaccct ggcgtgattg tgatcggcgg cgggctttcg aatgtggaac
    12961 tgcttatcac cgatctgaac gcagaagtcg ctccactggt tttcaccgat caattcacca
    13021 ctcccattgt aaaagcacag cacggcgaca gtagcggaat gcgtggcgct gcctggcttg
    13081 ctatgcgcaa cggagaagcc aatgaaacgt tcacaaatta attatgttat tgataaagca
    13141 catgccattg cgcagacctt ccgcgtttgt ttgccagagt tcgcctattt tacggtggat
    13201 gactggctgc agcgggaacg ggataactgg caagaagtgg tcgacttaca gctcggttgg
    13261 gatatcaccg attttggtcg tggtgatttc aaccaaacgg gactaacact gctaacgatg
    13321 cgcaacggcg cgctggggtc ggcggcatat ccaaaaccgt atgctgagaa aatgttgcag
    13381 atccaacaag atcagcagac tccctggcat tttcacaccc acaaaatgga agatatcctc
    13441 aatcggggcg gtggagactt atgcatgcag ttggcctggg ccaacgaagc caatctctgt
    13501 gatgatcaac gagtcattac cgtgagtgtt gatggtcagc ggcgtaccat gaaacctggt
    13561 gaaacgctgg tgctaaagcc ggggcagggc atttgcctgc cgccgcgtct gtatcatcgc
    13621 ttctgggcgg aaaaagcgtt tgttctcggc tgggaaatct cgatggtaaa tgatgaccaa
    13681 cacgataatt attttcttga gccgggagga cgttttccgg cgatagaaga agatgaaccg
    13741 gtgaaatggt tattgtgcag tgagtatgga attttgcgct aataacgggg aacgctagcg
    13801 ctgatatccg ctagcgtcct tatttttact gcaccggctc cgtcgacgta acttcaccac
    13861 tggaaactac gcccgcattc atggtttgag tcacttgctg ctttgcggca ttcacggcat
    13921 gcaattgccc ggtaaccgta ggtttcagcg ggacattggc ctgaatatcg ccactggcag
    13981 taagctgaat attgccgtcg ccagtcaacg gtaattcagg ccatccccac tgttgcaaaa
    14041 tattcaccgg cacaccgcgt ccattcaggc tgatatgggt ctgacgttgt ggcgtttgtg
    14101 aaacactggc ggtggcttcc agaatgcctt tttcagtaaa tgcactcagt tcgctgatat
    14161 tcaccgtgct gctgttggcg gtcagcgcca gcgatgggcg acgaacatca acacgattga
    14221 atgtcgcggc ggcggcattc agattcgccg aaccactcca gacgccccat ttatgatcgg
    14281 taaccagcgt caggttggca ccgtaaccat cgagcgcggt gagctgccac gggaagtcag
    14341 ggtcgatatc aatgatcaga ttgcggctgg cgctaaatct cttcagttgc aggctattta
    14401 accaaccggg tgtcgtttcc atccacaact gttgccagtt ttttggcaag gtatattcca
    14461 gcccggcaat tgccgcatca tcaaggagta acgttttccc gtcacgcaac cagctccctg
    14521 acgttctgac cataccccct tcccagcggc tggtgaactg gcgcagcgct acgccctgcg
    14581 gggaaaattc cgcgtttata atcgggtcaa ataaatgcag cgaaccataa atgaactcgc
    14641 tagcgttcat cgacagtttg ccttcctgtg tctgccagtc atctttactg aaggtcatgt
    14701 tgcgcaagct gagatcgagg tcggtcaccg cccagtccgg accttgcaaa cgagcgtcaa
    14761 tgacttccag gcgaccaatc tgcaacgacg ggacagagcg taatggcgca aagaagtcgg
    14821 ttagcgattt ttcgctttgt agacggatat ccgccatgcg cagattttcc acttgccagc
    14881 tgccgtcggc gttacgctgc gcgtttccgg ttaatgtccc gcgggcgatg tcggcaccca
    14941 agttagtcag cgtaacgcga tcgttatcaa tactgccttc aatcagtaca ttggtggcag
    15001 gaacatcatt gagcgaaagc gatccggcac taaactgaat ctgcgccttc gtacccagca
    15061 ctttaccggc ttctggtgac cacggaacca cgccaccatt tacccgctgc gcgcttagtt
    15121 tccattcgct attcgggcta ttaaacgcca tatcacgcag ttgcagacga tcggctttga
    15181 acggtagcgg cgcggtctgg tcggtgagat tcagcgtccc gttttccagc aagatggtat
    15241 cgacatggcg tggttcggtc agttggcgac tgcttagcgc aatgtcgaca cttttggcca
    15301 ccagggtcgc gggctgacca tcacgaccaa acgtgacgtt ctccagcacg atatgagatg
    15361 gcgcggaaaa acggtgatcc atcgccccga aggccagatg atagtcgcta ttctcggaaa
    15421 cccatgcgct gatatgttct gctccccagc gggtttgcag aagaaaatag aggccagcga
    15481 tcaccactaa cagagcgatg agaatgtaga gaagcagctt cccaataaat ttcatggtct
    15541 tccatcccgt gaaaagcaca taagggagtt atgcacgatt tacgcgcaat cctcaaggcg
    15601 ggaatggtga aagacgtgag actgcggcgg taattatctt accgccgcca gtgaattact
    15661 gtttttctgg tgggaaaatc agattcagca cgatggcagt aataccgccc gcggcaatcc
    15721 cggaggagag caggtttttc agccattcag gggcaaactg caaaatcagc ggctgctgag
    15781 agacgcccag accgaccgcc agcgacagcg cgataatcag aatcgcccga cggttcagcg
    15841 gctcacgaga aacgatacgc acaccggagg cggcgatggt gccaaacatt acaagcgttg
    15901 cgccgcccag aaccggttca ggaatgtgtt gtacaaaacc gctcactgcc gggaacagac
    15961 ccagcacgat caacatcagc gcgacgacaa aaccgacata gcggctggca acaccggtta
    16021 actggatcac tccgttgttc tgcccgaagc aggagttcgg gaaggtgtta aacaccgccg
    16081 aaacaaacga gttcaggccg tttgccagca caccgccttt caggcgtttc atgtacagcg
    16141 gaccggacac tggctgttcg gaaacgtcag aggtcgccgt gatatcgcca atggtttcca
    16201 gcgaagtgat cataaagacc agcatcagcg gcagcagcag actccattca atgccaagac
    16261 cgtaatagag cggcgttggc accataatca gttcttgtgt catcggttcg ttgctttctg
    16321 gcaacatgcc cataaaccac gccagcgcat atccggccgc catcgcaatt accagtgagg
    16381 ccacgcgtaa gtaagggtta cgttgacggt taagcaggat aattaaggct aaaaccacgc
    16441 ctgccagcag cagatttttc ggtgcgccga aggtgttatc gctcatggct gcgtaaccgc
    16501 cgccaatgga cgttaaccca acctgaatta gcgacaggcc gataatcatc accacaacgc
    16561 cagaaactag cggcgtaata atgcggcgcg ccagatgcag aacgcgggag atcaccatct
    16621 cagtgcaact tgccagcatc aacgtgccga acaaagccgc catcatggta ggaacatcag
    16681 caccaccggt tttcagcgcg gtaccgccca taatcagtgg ggcaacaaag ttgaagctgg
    16741 tgccctgaat agacaacagc ccggagccaa ccggacccca ggccttaatt tgaataatcg
    16801 atgccacacc ggaggcaaac agcgacatac taataatgtg ttgcgtgtct tgtgccggta
    16861 aacccagcgc ctggcagatt aatagcgctg gcgtgatcac cgcaacgaac atcgccagca
    16921 gatgctgaca ggcggcaaac agagtttgag gaagcggcgg acgatcttca agacggtaaa
    16981 tcagttcgct gttttgagtc tgcgcaaccg gttgcgcatt ttctgactcg agggtggaaa
    17041 cagacatcga aaacaatccc gttgtagcaa agcgggcatt ttagctgacc gaaagagaaa
    17101 agcaaacgtt tgccacggcc tctgctaaat atttaaatga gataaatgca cttttttata
    17161 taacttttgg tatttttctg cttaaaatcc atgccatttg ttcattaaca gctgagaatt
    17221 tgacccagga aagcataact cgatacccgt catagttcat aagttgccac tgcgcgatga
    17281 agtatgacga gtatgaaaga gtgatgcgga tacaaaggag taactatgtt tcatctcgat
    17341 actttagcaa cgcttgttgc cgcaacgctg acgttgctgc tcgggcgtaa gttggtccat
    17401 tccgtctcct ttttgaagaa atacaccata ccggaacctg ttgcgggtgg tttgttggtg
    17461 gcgctggcgc tactggtact gaaaaaaagc atgggctggg aagtcaactt tgatatgtcc
    17521 ctgcgcgatc cgttaatgct ggcattcttc gccaccattg gcctgaacgc caacattgcc
    17581 agtttgcgtg ccggtgggcg tgtggttggc atcttcttga ttgtggttgt tggtctgttg
    17641 gtgatgcaaa atgccattgg cattggtatg gctagcctgt tagggcttga tccgctgatg
    17701 gggctgttgg ccggttctat tacgctttcc ggcggtcacg gtacgggcgc tgcgtggagt
    17761 aaattgttca ttgaacgcta tggcttcacc aatgcgacgg aagtggcgat ggcctgtgcg
    17821 acgtttggtc tggtgctggg cggcttgatt ggcggtccgg tagcgcgcta tctggtgaaa
    17881 cactccacca cgccgaacgg tattccggat gaccaggaag tcccgacggc gttcgaaaag
    17941 ccggatgtgg gccgcatgat cacctcgctg gtgctgattg aaactatcgc gctgattgct
    18001 atctgcctga cggtggggaa aattgttgcg caacttttgg ctggcactgc ttttgaactg
    18061 ccgaccttcg tctgtgtact gtttgttggc gtgattctga gcaacggtct gtcaatgatg
    18121 ggcttttacc gcgtctttga gcgtgcggta tccgtgctgg gtaacgtaag cctgtcgttg
    18181 ttcctggcga tggcgctgat ggggctgaaa ctatgggagc tggcttcgct ggcgctgccg
    18241 atgctggcga ttctggtggt acagaccatc ttcatggcgt tgtatgccat cttcgttacc
    18301 tggcgcatga tgggcaaaaa ctacgatgcg gcagtgctgg ctgcgggtca ctgtggtttt
    18361 ggcctcggtg caacgccaac agcaatcgcc aacatgcagg cgatcactga acgttttggc
    18421 ccgtcgcaca tggccttcct ggtagtgccg atggtcggtg cgttctttat tgatatcgtt
    18481 aacgcgctgg tgattaagct gtatttaatg ttgccgattt ttgccggtta accgatgaag
    18541 cggcggtctt ctcaccgccg ccttttacgc attcgagtaa cgttccgttt ctggcatcca
    18601 gcgttctatc agggcttttg cctgctgtgg gtaacgttcg tgaatatggc gtgccaggcg
    18661 ctgaacttcc gggatcatcg cctgatcgcg cagtaaatcc gccactttaa attcagcatt
    18721 acccgtctga cgcgtgccta acaattcgcc agggccgcga atctccagat ctttttgcgc
    18781 aatcacaaac ccgtcgttgc tgtcgcgcag tacttgcaga cgaatttgcg ccgttttaga
    18841 gagcggcgtt ttgtagagca gcacgcagtg agaagccacc gcaccacgtc ccacgcgccc
    18901 gcgaagctgg tgcaactgcg ccagacccag acgctccggg ttttcgataa tcatcaggct
    18961 ggcgttaggc acatcaacgc cgacttcaat aacggtagtg gcaaccagca ggtgtaactc
    19021 gccttgttta aatgatgcca tgacggcctg cttctcggct ggtttcatcc gtccgtgtac
    19081 cagaccaaca ttcaactctg gtagcgccag tttcaactct tcccaggtag cttccgccgc
    19141 ctgcgcttcc agtaactctg actcttcaat caacgtacaa acccagtatg cctgacgacc
    19201 ttcagttatg caggcgtggc gcacgcggtc aataatgtcg gtacggcggg tatcaggaat
    19261 agcgaccgta gtcactggcg tgcggcctgg cggcagctca tctatcaccg aggtatcgag
    19321 atcagcatac gcagtcattg ccagcgttcg ggggatcggc gtggcggtca tgatcaactg
    19381 atgaggatgg aagccttgct gctggccttt ctcccataac gccagacgct gatgcacgcc
    19441 aaaacgatgc tgttcgtcga taatcaccag cgccaggccg ttaaactgca cctgttcctg
    19501 gaagatggcg tgtgttccga caatcatctg cacctgaccg ctggcgatgg cttcctgctg
    19561 tgccagtcgc gctttacctt tctgcttacc ggcgagccag cccacttcga taccgagcgg
    19621 tgcaaaccag ttgcggaagt tattcgcgtg ctgttcggcg agtaattcgg ttggtgccat
    19681 caatgccacc tgtttgccgt gcgcaatcgc acgcagcgcg gcgagggcag cgaccagcgt
    19741 tttaccggaa cctacatcgc cctgtaccag gcgcatcatc ggcgcatcca gcgccatatc
    19801 gcgctcgatc tccgccacca cgcgtgcctg tgcgcccgtt ggcttgaacg gtaaggctgc
    19861 gaggagttta tttttcagcg cgtcattggc gctaagcggc tgggcatgga aacgttgtgc
    19921 accagcgcgt aacgccaaca tgctgaggtt gtgcgcaagc agttcttcca gaatcagacg
    19981 gcgttgtgct ggatgctgcc cggtttcgag atcgctaagc tgtagcgtcg gcggcgggcg
    20041 gtgcaaagtg cgcaacgctt ccggtagcgt catcattcct tgtgacagtt ccggcggcag
    20101 gagttcttca atggcgcagg tgtcgagcag atccagcgcc tgatcggtta atttacgcag
    20161 cgtggcctgc tttacgcctt ccgttgttgg gtaaaccggc gttagcgttt cctgcaactc
    20221 tggcgtactg agatcgccct gcacgcggta ttccgggtgg atcatctccg caccatattt
    20281 accgcgcttt gcttcgccat aggccagcac gcggcggcct gtcgccaggc tatttttcat
    20341 tgccgcgttg aagttgaaaa agcgcatggt gagaatgccg gaaccgtcgc tgatctggca
    20401 ggtcatcatc cgccgaccgc cgaaggagat attgcagttc agcacttcgc cttccaccgt
    20461 ggcataaacg cccggtagta gttctccgat ggggtagaga tgggtgcgat cttcgtagcg
    20521 caggggaagg tgtaagagta gatcctgcac ggtatgcagg ttgattttcg ccagcttgtt
    20581 actaagcgct gcgccaacgc ccgttaggga actgagcggg acagcatcta acaggcgacc
    20641 tttcatggca cttaccctgc agcctgcatg gtagcccacc agtcagcatc ggcttcaatt
    20701 tcgccttgct gattaacgtg ggggtaaggc aggcctttgc gttttgcgac tttcgccagc
    20761 accggatagc cgccttcaaa cagcaggcgt tgttgctctg cttccggcaa catgctgttt
    20821 tcacgcaggt acatgcctgc attttgccgc tggcgctggg cttcataaag aatgagggct
    20881 gaggcaacgg aaacattcag cgactgcacc atgccgatca tcggtatgat gatgtcccga
    20941 tccgccaggg ccaacgcttc ctgcgtgatg cctgttttct cctgtcccat caaaatacag
    21001 gtcgggcgag tgtaatcaat ttcgcggaaa tcgacagcgt tatcagaaag atgggttgcc
    21061 agaatctgca tgccctggcc tttcagatga gcgacggcat cgccaatggt gcggtgcgtt
    21121 ttcacctgta cccagctgtt actacccgca gctgccgaag ccatggtgcg catccggcta
    21181 ccaggccaga cagcatgaac ttcatgtaca ccaacggcat ctgcggtacg aataatcgca
    21241 gaaacgttat gaggtttgtg gacctgctcc atgcagacgg tcagatcagg ctgccgcctg
    21301 gcgagcattt cgcagatgcg tgcataacgt gttgggttca taaaacatta atttcggttt
    21361 cgggtgactt taatcacgtc tggcatcacg cggattttgc gcatgatatt cgccagatgc
    21421 acacggtcac gggcggtcag acgaataaag gcgctgtaga cgcgaccatc tttctcttcc
    21481 gtattcaaac tttgaatatt cgaggtggtg gtgttaattg ccgctgtcag gtttgccagc
    21541 gcaccctgat gattgaacat ctccaccttg atttcggtga tgaactcctg cgccgtctct
    21601 ttatcccatt ccacagccat aaacttctct ggctctttct ggtagccacg gatgttacgg
    21661 caggattcat ggtggatcac cagaccttta ccggggctga cgtgggcgat aatcgggtcg
    21721 ccaggaatag ggcggcagca tttcgcaaag gtgatcagca cgccatcggc acctttaatg
    21781 ggcagatgtc cgtgactttg ggttgccggt ggaatggagg cgtccccatg ttgcagattt
    21841 ttcgcgacca ccacgctcat tgcgttacca aggccgattt cagccagcag atcgtcaagc
    21901 gttgccagct tcatgcggtc cagctcgcgc tgaatatttt cctgcgggat ttcattgagc
    21961 ttgcggctgc cacccaacgc atggttgagc agacgacggc ccaggcttac agaatcatca
    22021 cgcttgaggt ttttcagcaa ttgacgaatt ttggcgcgcg ctttcgagct aacgacaaag
    22081 ttcagccacg cggcattcgg gcgagcgccc ggtgcggtaa taatttcaac ggtttgaccg
    22141 ctggtaagcg gctgcgacag cgggtaaggc tggcggtcga cgcgtgcgcc cacgcaggca
    22201 tgaccgatat cggtatgcac cgcataagcg aagtcgacgg gcgttgcacc ggcaggcagt
    22261 tcgacaatgc gcccttctgg tgtgaaaacg taaatctcat ccgggaagag atcggattta
    22321 acgctctcga taaattcaaa cgagctaccg gcactctgtt gcagctccag caggctttgc
    22381 atccagcgct gggcgcgtat ttgcgcggta gtgctggttt cgccgtgctc tttataagcc
    22441 cagtgcgcgg caacacccat ctccgccatc tgatccatat cttcggtacg gatctggacc
    22501 tcaaccggca cgccgtgcgg gccaatcatc gaggtgtgca aagactgata gccgttcgct
    22561 tttggaatgg cgatatagtc tttcacgcgg cccggacgcg gcttgtacag gctgtgcatc
    22621 tggcccagca cgcgataaca ggtgtcagaa tcattgacga tcacgcggaa agcgtagatg
    22681 tccatgattg agtgaaaacg ctgctctttg agcaccattt tgcagtaaat cgaataaaga
    22741 tgcttttcgc gaccactgac gcggcacggt attcccgctt cctgcaaacg cccttcgatt
    22801 tcagaaagaa tcttctggat catctcttta cggttgccgc gcgcggcttt caccacttct
    22861 ttgattacgc gataacggtt gggatacagc gcctcaaaac ccagctcttc gagttcggtt
    22921 ttaatgtggt ggatacctaa acggtgcgcc agcgggctgt aaatttcgag ggtttcacgg
    22981 gcgatgcggc gacgtttgtc cgggcgaagt gagcccagcg tgcgcatgtt gtgggtacgg
    23041 tcggcaagtt tgatgagaat gacgcggata tcctgcacca tcgccataat catcttgcga
    23101 aagttttcgg cctgcgcctc tttcttatcg cggaacttga gcttatcaag tttcgacacc
    23161 ccctctacca gctcggcgac gcttttacca aaaagctgtt ccatgtcctg gtaggtggcg
    23221 ggagtatctt caatcacgtc atgcagcagc gccgccataa gcgtttcata gtcgagtttc
    23281 atctcggcca gaatgcaggc aaccgccacc gggtgcgtga tatagggttc accgcttgaa
    23341 cgtgtttgcc cctcgtgagc atcacgtgca acgagatacg cctgccgcag acgcttgatt
    23401 tggtcttccg gcaggtaggt ttgaatcagt tgattcaggc tttcaaacag atacaagggc
    23461 gacccgcttt gtgattaacg acgaccttca gcaatagcgg taacggcttg taattcagcg
    23521 gcttcctgct cttgctgttc ctggcgttcg cgaacgtcga ggatctggtt gttgatcaga
    23581 ccttcttcga tttcgcgcag cgcgattaca gtggttttat cgttttcttc cggtaccagc
    23641 ggatcctttc cgcctacctg catctgacga gcgcgacgcg cggcgaccag taccaggtca
    23701 aaacggttac caattttctc tacagcgtcc tgaacagtta cgcgtgccat acttaaaaag
    23761 ctccacaggt gaagaaatga ctgggcatga tactgaaatc aggttcagtc tgccaacaat
    23821 ttgctgatta aagcgtcatg acgctgcttt tggcggctca tgcgcagacg ttcggcgcga
    23881 ataatggtct tcaaatcggt caacgcggta tcgaagtcat cattcacaat caggtaatca
    23941 tattcggcgt aatggctcat ttctgcaaca gcttgcgcca tacgctttgc aatgacctct
    24001 tcgctgtcct gaccgcgacc gcgtagacgg cggtccagtt caattttgga cggcggtaaa
    24061 ataaagatac tccgcgcgtg cggcatcttc tggcgaattt gctgcgcacc ctgccagtcg
    24121 atatcgagaa aaacatcgac accggtcgcc agtacttgct caatggcctc acgcgaagtg
    24181 ccatagtaat taccaaaaac ttctgcgtgt tcgaggaacg catctctgct aatcatttct
    24241 ttaaattcat catgattaac aaagaaataa tgttcaccgt ggacctcacc aggacgcggt
    24301 tggcgtgtgg tgtgtgaaac agaaacctgg gtgtcataca acggttgggt ttttaataaa
    24361 gcctgaatca ggctggattt acccgcgcca ctgggggcag aaacaatata aagcgtgcct
    24421 tgagccatga gtatctttcg tatgtgatta gcgaaataaa gcctacatac gagcttatta
    24481 tacacggcgc ggccatgtga cgtagccctt gctcacactt tttgcgcaaa gttgttgcgt
    24541 tttgcgtagc tgcttccgca tttacctgtc attcactgca ataaagacga ttctcaggaa
    24601 gcgtcctcgc agactccgtt tttgccactc ggctttgctt tcatcacgtt gtatatctgc
    24661 ctgactatct acagcggagg tgatgatgaa agtatggatg gcgatattaa taagtatctt
    24721 gtgctggcaa tcatcggcgt gggcagtctg tccggcctgg tcgccagcca gagcacagga
    24781 agaaatttcc cgcctgcaac agcaaataaa acagtgggac gatgactact ggaaagaagg
    24841 gaaaagtgag gtggaagacg gtatttacga tcagttaagt gcccgtctca cgcagtggca
    24901 acgctgtttt ggtgaagaga cgcatcgcga cgctatgatg ccgcctttaa acggtgcggt
    24961 tatacatccg gttgcacata cgggtgtacg caaaatggcg gataaaatcg cattaagtct
    25021 gtggatgcgc gaacgtagcg atctttgggt gcagccaaaa gttgatggcg ttgcggtaac
    25081 cctggtttat cgggacggga aactgaacaa agcaatcagt cgcggcaatg gcctgaaagg
    25141 cgaggactgg acgcagaaag ttcgcttaat ttccgctgtg ccgcaaaccg ttagcgggcc
    25201 tttagccaac agtacgcttc agggggaaat atttctcaaa cgcgaggggc atatccaaaa
    25261 acaaatgggg ggaataaatg cccgcgcaaa agttgctggt ctgatgatgc gccaggacga
    25321 tagcgacacg ctgaattctc tgggcgtttt tgtctgggca tggccggatg gaccgcagtt
    25381 aatgaccgat cgtttaaaag agctggctac cgctggtttt actctaacgc agacgtatac
    25441 ccgtgcggtg aaaaatgctg atgaagttgc gcgcgtacgc aatgcgtggt ggaaaacgaa
    25501 attacccttc gtcaccgatg gcgtggttgt gcgagcggcg aaagaacccg aatcccgcca
    25561 ttggctacca ggccaggcag aatggctggt ggcctggaaa tatcaacctg tagctcgggt
    25621 tgccgaagtg aaggcgattc agtttgcggt gggtaagagc ggtaaaatat cggtggttgc
    25681 gtcactcgca cctgtcatgc ttgatgataa aaaagtccag cgggtgaata ttggttccgt
    25741 caggcgctgg caggagtggg atattgcgcc tggtgatcag atcctcgtca gccttgccgg
    25801 tcagggtatt cctcgcattg atgatgtggt gtggcgcggt gcagaacgta caaaaccgac
    25861 accgccagaa aaccgcttta actcgttgac ctgctacttt gcttctgatg tttgtcagga
    25921 acagttcatt tcacgcttag tctggctggg gtcaaaacag gttcttgggc tggatggcat
    25981 tggtgaggcc ggctggcgct cgctgcatca gactcatcgc tttgagcata tcttttcctg
    26041 gcttttatta acgccagagc aattacagaa cacgccgggg atcgcgaaaa gtaaaagtgc
    26101 gcagctatgg catcagttta atctggctcg taagcagcct tttactcgct gggtgatggc
    26161 aatgggaata ccgctaaccc gggcggcgct taatgccagt gatgaacggt cctggtcgca
    26221 acttttattg agcacggagc agttctggca gcagctgccg gggactggat cggggcgtgt
    26281 cagacaggtt attgaatgga aggaaaatgc gcaaatcaag aagctgggca gttggctggc
    26341 tgcccagcag atcacaggtt ttgaacctta gtgtccttcg tggctgtagt aaaagaccgg
    26401 taatcccagc ttaaggcgta gcgccagtaa gcgggcaaaa aagccgaata ccagggtgga
    26461 gataattacc acatcatggt tagaaacata gtgttgcagc gcgatgtaca gcacggcgga
    26521 ggcaaatgag acaccggcat acaactcttt ctggaatacc agcgggatgc gtttacagaa
    26581 catatcgcgc aaaacgccgc caaatacgcc ggtcgtcaca gctgcgacaa cggcaatgat
    26641 cgggccgtgt cccatatcca gtgcaacctg tgcgccaatg atagaaaaga ccaccagtcc
    26701 gagcgcatcg agcaccagaa acactttgcg caggtaaggc attacaggag caacgatagt
    26761 ggtgagtact gcggcggtag cgacaataat cacatattcc gggtgtttga cccagccgag
    26821 cggatagtgg cccagcagaa tatcgcgcac tgaccctccg ccaattgccg tggcggtcgc
    26881 aataataatt acgccaaatg tatccatgcg ccgtcgtccg gccgccagcg cccccgtcat
    26941 ggcttcggca gtaatgccaa ccaaatacaa aatgtgtaac agcatggttc tctccctaaa
    27001 aatctgtctg gcagggtagc gatttgtgcg tattgtcacg attgagattt tttaaggtga
    27061 gaggaaattg ataacttgta gttatgtgaa atcgctattt tctgtagcgt agatgcattc
    27121 ttctgacttc ttgtctgaga aaatgcataa gaaaaaatag gttaaaattg aggggtggaa
    27181 aagaaaggtg caatgtttag ccattgtgtt caccgcacct ggactttggc ttacgcatcg
    27241 ggtatctcaa tcaacaaaaa gagtttttag tttctgctat ttcttctctg taatttttac
    27301 actgttttcc aactgcttga tgttgacctg cggggtcggc aactcctcag gcatggttcc
    27361 gccaagttcc tgaatggttt gcctcacctt gcgacccacg tcaaagtgtg tggtgttagc
    27421 ttgctgtttt gaattcacgc catcccgctt gagtttttct tctgtttggg tagctcgaaa
    27481 gagattagcc gccagttctg ttgaacccat atgatccagg atcttctgac tctttttcag
    27541 ccccttccgc tgatggatgg ctttctgatc taatccacca tacagccccc ggtaaccatg
    27601 attctgaaat atggcgaaat cgatagctgt cgctacccca gcttgctgcg ctgcctcaac
    27661 taattgtttg ttatgttctt tcaactcatt tctcagaaaa agacgctttt cgtcttcacg
    27721 aagttgcctg aaagcttcat cgtcagccag ctcctgccgt cgggtctgga tagcaaaata
    27781 agtttgccct gccgcaataa ccggtttcgc agggtcgccg ttttgtacta ccaggtaaca
    27841 ggcatagcgg gagagtacga tgtcttttaa tgctcttttg gcatttgagc caattttgac
    27901 catatcgagg atctcctcga aatggtcatc aattgtgtga ccactattca gacaagcttc
    27961 tttggcgcgc gttaatacag gtataaagtg acgatactct gaataaccca gtagttttcc
    28021 aagttgcctt gctgaccatt gttctgctcc gtttgcatta atcagcttta tctcttcaaa
    28081 aggttgatga tgttcgttca ttgcccactc catgtgatat cgattgcaag gaaagtaagt
    28141 ctacaactgt gtttatatac agttgtaagt atctcacgtt ctcactgcgc tgggcaaaaa
    28201 aacatcgcaa aatctggagc aggcacgcaa aacagaattg aatgttgcgg ggaggaaaaa
    28261 tgccgctctg tttaagtggg cggcatttgt gaaggctgaa aaggaagaat tggcgttacg
    28321 agttattcaa tgttctggat ctgctcgcgc atctgctcaa tcaacacttt cagctcgatg
    28381 gcagaattcg tcacttcggc attgatagat ttcgacgcaa gagtgttcga ctcgcggttg
    28441 aactcctgca tcataaagtc cagacgacga ccaaccgctt cttttttctt cagaatgttg
    28501 taggtctctt tgacatgcgc ttcgaggcgg tccagttctt cggcaacgtc aattcgttgt
    28561 gccagcagaa ccaactcctg ctccagacgg ttgttttcca gttgcacctg agcatcttcc
    28621 agcttcgcga ccagacgctc acgctgccat tgcaggattt ccggcatatg ggcgcggact
    28681 ttgaccactt cggcggtgac gccttccaga cgctgctcga tcaatgcttt cagcgcctga
    28741 ccttcggttt cgcgcgcgac gataaagtcg tccagcgtac catccaacgc cgcgagaatt
    28801 tcagcggcaa tcgcgtcgag atcctgctcc tgggctgcca tcacgcccgg ccagcgtaga
    28861 atatcaaccg ggttgatttc cccttcgtca ctctgcattt ttacccagtt cgcggcagtt
    28921 accagctgtt tagccagttt ttcgttgagg atcagctcac cttgcgcgct aacatctggc
    28981 tcatagcgca gggtacattc cactttaccg cgcgtcaggc gagaacgaat acgctcacga
    29041 acgacaggtt caaggctacg gaactgctcc ggcagacgaa agtaagtttc cagataacgc
    29101 tggtttaccg agcgcatttc ccaggttgcg ctcccccatt cacccttgat ttcacgccgg
    29161 gcgtaggcgg tcatactgcg gatcatagac gttcctgttt ataaaaggag aggtggaagg
    29221 attatagcca tcgatgcctt gtcaggatag gaataaccgc cggaagtccg tataatgcgc
    29281 agccacattt gtttcaagcc ggagatttca atatgcgtcc agcaggccgt agcaataatc
    29341 aggtgcgtcc cgttaccctg actcgtaact atacaaaaca tgcagaaggc tcggtgctgg
    29401 tcgaatttgg cgataccaaa gtattgtgta ccgcctctat tgaagaaggc gtgccgcgct
    29461 tcctgaaagg tcagggccag ggctggatca ccgcagagta cggcatgctg ccacgttcta
    29521 cccacacccg taacgcgcgt gaagcggcga aaggtaagca gggtggacgc accatggaaa
    29581 tccagcgtct gatcgcccgt gctcttcgtg cggcagtaga tttgaaagcg ctgggtgagt
    29641 tcaccattac gctggactgc gacgtgcttc aggctgatgg tggcactcgt accgcgtcga
    29701 ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa gctggtggaa aacggaaagc
    29761 tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc tgtcggaatt gtgaacggcg
    29821 aagcggtttg cgatctggaa tacgttgaag actcagccgc agagaccgac atgaacgtag
    29881 tgatgaccga agacgggcgc atcattgaag tgcaggggac ggcagaaggc gaaccgttca
    29941 cccacgaaga gttactcacc ttgttggcgc tggcccgagg gggaatcgaa tccattgtag
    30001 cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg actgatgagt cgcctttttt
    30061 ttgtctgtag aaaagtaaga tgaggagcga aggcatgaaa ccatatcagc gccagtttat
    30121 tgaatttgcg cttagcaagc aggtgttaaa gtttggcgag tttacgctga aatccgggcg
    30181 caaaagcccc tatttcttca acgccgggct gtttaatacc gggcgcgatc tggcactgtt
    30241 aggccgtttt tacgctgaag cattggtgga ttccggcatt gagttcgatc tgctgtttgg
    30301 ccctgcttac aaagggatcc cgattgccac cacaactgcc gtggcgctgg cggagcatca
    30361 tgacctggac ctgccgtact gctttaaccg caaagaagca aaagaccatg gtgaaggtgg
    30421 caatctggtc ggtagcgcgt tacaaggacg cgtaatgctg gtggatgatg tgatcaccgc
    30481 cggaacggcg attcgcgagt cgatggaaat tattcaggcc aatggcgcga cgctggctgg
    30541 cgtattgatt tcgcttgatc gtcaggaacg cgggcgcggc gagatttcgg cgattcagga
    30601 agttgagcgt gattacaact gcaaggtgat ctctatcatc accctgaaag acctgattgc
    30661 ttacctggaa gagaagccag aaatggcgga acatctggcg gcggttaagg cctatcgcga
    30721 agagtttggc gtttaaagaa actcgccgga tgaaaagtca tccggcgtca tattactgca
    30781 actgtgcggc aattagcggc cagcgggcgt caaaatcatc cgtcgggcgg tatttaaact
    30841 cgctgcggac aaagcgtgac agcatacctt cacagaaggc cagcaactgg cttgccagca
    30901 gggtttcatc ggtggtgtaa ccttcaccct cacgcattct cttttcacgc aatacctgac
    30961 gcaactgcgc ttcaatacgc tcgaacagct ggttgatgcg cccttgcaga cgatcctgtt
    31021 cgaacattag cgcatgacca gtgaggatgc gagtcaggcc aggattacgc tcaccaaaac
    31081 cgagaagcag caacacaatc agacgcaggc gcgctgtggt gtccttctca tctttcagaa
    31141 tcagattgat gcgagtaatc aggctatctt cgataaactc aatcaggcta tcgaacatgc
    31201 gggtcttact ggggaagtgg cgatacagtg ccgcttcgga aacgccgaca gaggcggcca
    31261 gttttgccgt cgtgatacgt tggcttccat cgctggattc cagcatcagc gccagagact
    31321 gaagtatttc ctcgcgacgg ttccttttcg cagtttgttt ttctgccatg ttacaaaata
    31381 cccctgaaaa taagcacttg ccaggcggca cccacgctat gaccgcaaac gaaatgtttg
    31441 cggctatgtt atgacgttat tcggatgcgt atgtgttact gacgaccaga gtgaccaaag
    31501 ccgccttcac cgcggtcggt ggcgtcgaaa tcttccacca gattaaattc agcctgtact
    31561 accggaacaa aaatcatctg ggcgatgcgt tcgccaggtt gaatggtgaa gctgtcctga
    31621 ccacggttcc acacggaaat catcaactgg ccctgatagt cagaatcgat caatcctacc
    31681 aggttaccaa gcacgatacc gtgcttatgt cccaatccgg agcgcggtag catcattgcc
    31741 gccagtgaag gatcggcaat atgaatcgcc agcccggtcg gaaccagcgt agtgtcaccc
    31801 ggagccagtt ctacggcgtc gtcgagacag gcacgcaggt caagtccggc agagccagag
    31861 gtggcataag tcgggagcgg aaattccttc ccaacgcgcg ggtccagaat cttaacgtcg
    31921 atttttttca tcataacggg tcacgatctc gtcgagtaat aattggccaa ggagctcttt
    31981 gcgctcaagc ggtaagactt tatctccgtc ctgccagaaa aggtgtaatg cgttgttgtc
    32041 gctgttaaat ccttgagttg gctgggaaac atcgttcgcg cagatcagat caaggttttt
    32101 acggatacgt ttttgccggg cgtattcttc cacattattt gtttcggcgg caaatccaac
    32161 gacgtagggg cgatggtctt ttagtgcggc aacgcctgcg acgatatcgg ggtttttaac
    32221 catttttatt gttaattcat caccctgcgt ggcctgcttt ttgatttttt ctggggccac
    32281 ggtagctgcg cgataatccg ccacggcggc gcaaccgata aaaatatttt gctgctgtac
    32341 agatgcattc acggcggctt ccatttccag cgcggtcatc acatcaacac gtttaacaaa
    32401 cggtggcgtc ggtagtgaaa ccggacctga taccagcgtg acgttcgcgc cacgacgggc
    32461 agcggcggcg gcgatagcaa aacccatctt gcctgagctg tgattagaga tataacgcac
    32521 cggatcgagt ggttcacgcg tcgggccggc ggtaatcata atgttcagat gtttcaggtc
    32581 gttgacgggc gaaaaatgcg ctaccgccat atcaacaatg gttaacggat cgagcattcg
    32641 cccagggccg atatcaccac aagcctgact gccactgtct ggcccccaaa tgagcaaacc
    32701 acgggaagca agcacctcta aattatgctg cgtggcagcg gcacggtaca tctgctggtt
    32761 catggcgggg agcacggcta caggcgcagg tgtggccaga caaatcgtcg ataccagatc
    32821 attcgccatt ccggcagcaa cacgggcaat caaatctgcc gtggcagggg cgagaatcac
    32881 taaatcagcc catttaccca gctcaatatg gcccatagcg gcttcggctg ctgggtccag
    32941 cagactgtcg gaaacgggat aaccagaaac cgcctgcaag ctaagtgggg tgataaaggc
    33001 ttttgccgct tcggtcatgg ctacgcggac gtcggccccg cgctcgcgca aacgacgcac
    33061 cagttcaggg gttttatagg cagcaatacc gccgctgacg ccgagaacga tttttttacc
    33121 ggccaggctc atgatgattt ttcctgttgg gtgacaccag aagttggcga ttttatcaca
    33181 atacttttgt tgtcgtgcct tcacctgaga atcactttgc gaggcgcttt ccaggattga
    33241 aaactggccg tcgatttaac ggaacggcta tgacaggatg cgagcaccac aaaggaggtg
    33301 aaggtgaaaa acaattcaca gctgttgatg ccgcgcgaaa aaatgctgaa gtttggtatt
    33361 agcgccttaa cggatgtcga gctgctggcg ctatttctgc gtaccggaac gcgcggtaaa
    33421 gatgtattaa ccctggcaaa agagatgctg gagaatttcg gctctcttta tggcttgtta
    33481 acctctgaat atgaacaatt tagtggtgtt catggaattg gcgtggcgaa atttgcccag
    33541 ttaaagggga ttgccgaact ggcgcggcgt tactacaacg tgcggatgcg tgaagaaagc
    33601 cctttactca gcccggagat gacgcgggaa tttttacaaa gccagctcac gggtgaggag
    33661 cgggagatct ttatggtgat ctttctcgac tcacaacaca gggttataac gcatagccgt
    33721 cttttttccg gcacgctaaa ccatgttgaa gtccatcctc gggaaattat ccgcgaagcg
    33781 ataaaaataa atgcctcggc gctgatcctt gcacataatc acccttcggg ttgtgctgaa
    33841 cccagtaaag cggataaact cattactgaa cggataataa agagttgtca gttcatggat
    33901 ttacgcgtgc tcgaccatat cgtgattggg cgtggagagt atgtttcttt tgccgaacgc
    33961 ggctggattt aacccgctat gcgcgatcct tcgggatctt tgtctgttcg ggacttgagc
    34021 acatcgctga gtcagcgtat actacgccac ctttgagaat ctcgggtttg gcatttgggc
    34081 ctggcaatcg agagttcaca tagaactgcg atgaccgggc tgtaaagcct gacgaggcgc
    34141 caatacccca tacgaagctc gagctaattt gatttttgga gaatagacat gtcccgagtc
    34201 tgccaagtta ctggcaagcg tccggtgacc ggtaacaacc gttcccacgc actgaacgcg
    34261 actaaacgcc gtttcctgcc gaacctgcac tctcaccgtt tctgggttga gagcgagaag
    34321 cgttttgtca ccctgcgcgt atctgctaaa ggtatgcgtg taatcgataa aaaaggcatc
    34381 gatacagttc tggctgaact gcgtgcccgt ggcgaaaagt actaagtact tagaggaaat
    34441 aaatcatggc taaaggtatt cgtgagaaaa tcaagctggt ttcttctgct ggtactggtc
    34501 acttctatac cactacgaag aacaaacgta ctaagccgga aaaactggaa ctgaaaaaat
    34561 tcgatccagt tgttcgccag cacgtgatct acaaagaagc gaaaatcaaa taatttttgc
    34621 tttgatgtaa cgaaaaacct cgccccggcg gggttttttg ttatctgcct gcccccatat
    34681 tgactgcatc tgttcattcc tggagatgct atgcctgaat tacccgaagt tgaaaccagc
    34741 cgtcgcggca tagaaccgca tctcgttggt gcaaccattc ttcatgcggt ggtgcgcaac
    34801 ggacgcttgc gctggccggt ttcagaagag atctaccgtt taagcgacca accagtgctt
    34861 agcgtgcagc ggcgggctaa atatctgctg ctggagctgc ctgagggctg gattatcatt
    34921 catttgggga tgtctggcag cctgcgcatc cttccagaag aacttccccc tgaaaagcat
    34981 gaccatgttg atttggtgat gagcaacggt aaagtgctgc gctacaccga tccgcgccgc
    35041 tttggtgcct ggctgtggac caaagagctg gaagggcaca acgtgctggc ccatcttgga
    35101 ccggagccgc ttagcgacga tttcaacggt gagtacctgc atcagaagtg cgagaagaaa
    35161 aaaacggcga ttaaaccatg gctgatggat aacaagctgg tggtaggcgt agggaatatc
    35221 tatgccagcg aatctctgtt tgcggcgggg atccatccgg atcggctggc gtcatcactg
    35281 tcgctggcag agtgtgaatt gttagctcgg gtgattaaag cggtgttgct gcgttcgatt
    35341 gagcagggcg gtacaacgct gaaagatttt ctgcaaagtg atggtaaacc gggctatttc
    35401 gctcaggaat tgcaggttta cgggcgaaaa ggtgagtcgt gtcgggtgtg tggtacgccg
    35461 attgtggcga ctaaacatgc gcagcgggca acgttttatt gtcggcagtg ccagaagtaa
    35521 ttgatgcgcg ccggatggca taccatccgg cataaactct acgctaactt cgccatcagc
    35581 gcctgatgga cattctccgg caggaaatgg gtgacatcgc cctgatggcg cgccacctct
    35641 ttcaccaacg atgaagagat aaacgaccac tctttcgacg gcatcagaaa cacactttcc
    35701 agttccggca ttaagtggcg attcatatgc gccagctgca tttcatattc aaaatccgcc
    35761 accgcacgca ggccacgaat cagcaccgta gcgtgttgat tacgggcgaa gttcgccatc
    35821 aaatcactaa acccgaccac ttccacgttc cccagatgcg cggttgcctg ctgtgccagc
    35881 gccacacgct cttccagagt aaacatcggt tttttactgg gactggcggc gatcgccaga
    35941 ataacgtgat cgaacatttg cgtggcgcgc gtcacgatat cgatatgacc attggtaatg
    36001 ggatcgaaag tacccggata aatcgcccgt ttttgcataa caacctcaat gcgttttcgg
    36061 tggcaggtaa ggttccagca gttgaagcag acgctgtagc gcgccctggt tttgatacag
    36121 tacttcaacg gcatgacggc catagaaact acggtaatcg gcgtcggtga gtaaagagga
    36181 aacctctttt gcaagcgacg tggcatcggt aacggtaatc agcccgcttg cctgctccag
    36241 ccgcgcgcaa atgtctttaa agttaaaagt atgaggcccc atcaataccg gaatagcgtg
    36301 tgcggcagct tccagcggat tatgcccacc acgttcaacc agtgaaccgc caacaaaggc
    36361 gagatcggca atgccataca gtaacatcaa ctcgcccatc gtatcgccaa ccacaacctg
    36421 cgtgctggtg gaggggactt cccctgaaga gcgtgtgata tagcttagtc cagcctggcg
    36481 gacaaggtta atcgcatccg ggaagcgttc cggatgacgg gggaccagga tgagcaataa
    36541 attcgggaat tgctgtaaca atgcctgatg tgcggcgatc accacactct cttcgccttc
    36601 gtgagtgctg gtggcaatcc ataccgggcg gtgtggtgcc cactggcggc gcagcgtcac
    36661 ggctttagca gccaactgcg gcgttacaga aatatcgaat ttcaggctac cggtaacggt
    36721 tacctggtta ttttttgcgc ccagcgccac aaaacgtgca ccatcttctt cattttgcgc
    36781 agcaatcagc gtaatacgac gcagcaagcg acggacgaat ttacccagtt tggcataacc
    36841 tgcggccgag cgggcagaga gtcgcgcgtt agcgatcacc agcggaattt tacgtttatg
    36901 tagcgccgca atcaggttag gccatagttc ggtttccata atcaacacta gtttagggtc
    36961 gactttattc aggaaacgat taagtgcatc gggcaggtca tatggcagat aaacgtgctg
    37021 aacatccttc ccgaaagccg attgtacgcg ctccgaaccg gttggcgtca tggttgtaac
    37081 ggtaatcggt aaatcaggat aacgatggcg cagcgcgcgc accaacggga tcgccgccag
    37141 agtttcaccg acggagacgg agtgcagcat gatgccgcct ggttttagcg gatggcggta
    37201 aaaaccgtaa cgttcacccc agcgttttcg ataggccgga gccttacgtc cgcgcaccca
    37261 gagccgtatc cagatcagcg gctgaataag gtagagaagg gcggtgtaaa gcaattcgag
    37321 catagtaaat agctgactta tggatgtgct ggggattcta tgtatttagc tgtggcttta
    37381 ccattacttt tcccgttttt gacttaaata gcaatttacc cgccaattct tgctgaacat
    37441 ctatactttt tttgtatttt tatgcgattc attgatttct gcagtacgtg caggaaggaa
    37501 tcgcagagca acagtgaaag aatatggttg ctaatgattt tatttgattg atttctttat
    37561 tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat tgccgcgaaa gaagtgatac
    37621 actagcgcgt caatatcagt gcggtatcag tattcaggta gctgttgagc ctggggcggt
    37681 agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa caaaaagagt tacttgtgga
    37741 taagccattt cgaagaattt tgctcattaa gatgcgtttt catggggata tgttattaac
    37801 tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac gcaaaaatcg atgtgctgct
    37861 ttatcaggac accatcccga tcctgtctga aaatccagag attaacgcgc tctacggcat
    37921 aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac tttttccatc tcatcaaggt
    37981 attacgtgcc aataagtatg accttatcgt taatcttacc gatcaatgga tggttgctat
    38041 actggtgcgc ttattaaatg cccgtgtgaa aatttcccag gattatcatc atcggcagtc
    38101 tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg cagggtggaa atgtggtgga
    38161 aagtaactta tccgtgctga caccactggg agttgattcg ttggtgaagc agacaaccat
    38221 gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa cttgatcacg ctggtgttgg
    38281 acaaaattat gtggttatcc aacctacggc gcggcaaatc ttcaaatgct gggacaacgc
    38341 caagttttcc gctgtgattg atgccttaca tgctcgtggt tatgaagtcg ttctgacgtc
    38401 cggcccagat aaagacgatc tggcctgcgt caatgaaatt gcgcagggat gccagacgcc
    38461 accagtaacg gcgctggccg gaaaggtgac cttcccggaa cttggtgcgt taatcgatca
    38521 tgcgcagctg tttattggcg ttgactccgc accggcgcat attgccgctg cagttaatac
    38581 gccgctgata tcgctgtttg gtgcgacaga ccatattttc tggcgtccct ggtcaaataa
    38641 catgattcaa ttctgggcgg gagattaccg ggaaatgcca acgcgcgatc agcgtgaccg
    38701 aaatgagatg tatctttcgg ttattccggc ggcagatgtc attgctgctg tcgataaatt
    38761 actgccctcc tccacgacag gtacgtcgtt atgatcgttg ctttttgttt atataaatat
    38821 tttccctttg gcggtttgca gcgcgatttt atgcgtattg ctcagacagt cgccgcccga
    38881 ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg aatgccctga tgtatttgaa
    38941 ctgatcaaag tgccggttaa atcgcatacc aatcacgggc gcaatgcgga gtattttgcc
    39001 tgggtgcaaa aacatttacg cgaacatccc gtcgataaag tcgttggatt caacaaaatg
    39061 ccggggctgg acgtttatta tgccgctgat gtttgttatg ccgagaaagt agcgcaggaa
    39121 aaaggctttt tctatcgcct gacgtcacgt tatcgccatt atgccgcctt tgagcgggca
    39181 accttcgaac agggcaagcc gacacagctg ctgatgctga cagataagca aatcgccgat
    39241 ttccagaaac attatcagac tgaagcggag cgttttcata ttctgccacc ggggatttat
    39301 cctgatcgta aatatagcca gcagccagct aatagccgtg aaatcttccg taagaagaat
    39361 ggaataaccg aacaacaata tttattgttg caggtcggtt cagacttcac gcgtaaaggt
    39421 gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt cgctgcgcca caacacattg
    39481 ctatatgttg ttgggcagga taaaccgcga aaatttgagg cgctggcaga aaaacgcggc
    39541 gtgcgcagta atgttcactt cttctcgggg cgcaacgatg tctcggaatt aatggcggcg
    39601 gcggatttat tactgcatcc tgcctaccag gaagcggcgg gaattgtgct gctggaagcg
    39661 attactgcag gattaccggt actaacaact gccgtttgtg gctatgcgca ttatattgtc
    39721 gacgccaatt gcggcgaggc tattgctgag ccattccgcc aggaaacatt gaatgagatt
    39781 ttacgcaaag cgttaacgca attttcattg cgccaggcct gggcggaaaa tgcgcgacat
    39841 tatgctgata cacaagattt atacagtctg ccagagaaag cggcggatat cataacgggt
    39901 ggtctggatg gttgaactta aagagccgtt tgccacgtta tggcgcggaa aagatccttt
    39961 tgaggaagtt aaaactttgc agggtgaggt atttcgtgaa ctggaaactc gccgtactct
    40021 gcgctttgaa atggcgggca aaagctattt tctcaaatgg catcgcggca cgaccctgaa
    40081 agagataatc aaaaatttac tctcattgcg gatgccagta ttaggcgcag accgcgaatg
    40141 gaatgcgatt catcgactgc gggatgtcgg cgttgatact atgtatgggg tggcattcgg
    40201 cgaaaaaggc attaatccgc tcaccagaac ctcgtttatt ataaccgaag atctgacacc
    40261 aaccatcagt ctggaagatt actgtgctga ctgggcgact aaccctccag atgttcgcgt
    40321 aaagcgtatg cttattaagc gtgtcgcgac gatggtgcgc gatatgcatg ctgcgggcat
    40381 taaccaccgt gactgttata tctgtcattt cctgctgcac ttgccttttt ccggtaagaa
    40441 agaggagtta aaaatttcgg taattgatct gcaccgggcg cagcttcgca cgcgcgttcc
    40501 acgtcgttgg cgcgataaag atcttatagg gctttatttt tcttcgatga atattggcct
    40561 gactcagcgg gatatctggc ggtttatgaa agtgtatttt gtcgcctcgc ttaaagacat
    40621 tctcaagcag gaacaaggac tgctgtcgca agcagaagca aaagccacaa aaatcaggga
    40681 aagaacgatt cgaaaatcgt tgtaatttcc ggagagaaat atgagtgccc actattttaa
    40741 tccacaagag atgatcaata agacaatcat cttcgatgaa aggccagcgg cgtcagtggc
    40801 atcatcattc catgttgctt atggcattga taaaaacttt ctttttggtt gtggtgtttc
    40861 aatcacgtca gttttgttac ataacaacga cgtaagtttt gttttccacg tttttattga
    40921 tgatatccct gaagccgata tccagcgttt agcccaattg gcgaaaagct atcgtacctg
    40981 tatccagatc catctagtaa attgtgaacg gcttaaggca ttaccgacga ccaaaaattg
    41041 gtctattgcc atgtatttcc gttttgtaat tgcagattac tttattgatc aacaagataa
    41101 gatcctttac ctggatgctg atatcgcctg tcagggaaac ttaaagccgc tgataacaat
    41161 ggatcttgcc aataacgttg ctgctgttgt tactgaacgc gatgctaact ggtggtcgtt
    41221 acggggtcaa agtctgcagt gtaatgaact tgaaaagggt tactttaatt caggtgtcct
    41281 gttaattaat acactagcgt gggcgcagga gtccgtttct gctaaagcga tgtcgatgct
    41341 tgctgataaa gccatcgttt cccgtttaac ctatatggat caagatatcc ttaatcttat
    41401 cctgttaggg aaagttaaac tcattgaagc taaatacaat acgcaattta gtttaaatta
    41461 tgaattaaaa aaatcatttg tttgtccaat taatgatgaa accgtattaa ttcattatgt
    41521 cggcccgaca aaaccctggc attactgggc cggttatcca agtgcgcaac cttttatcaa
    41581 agccaaagaa gcatcgccct ggaaaaatga accgttaatg cggccagtta actcaaacta
    41641 tgctcgttat tgcgctaagc ataatttt
//
LOCUS       DAZKJR010000025        41352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKJR010000025 DAZKJR010000000
VERSION     DAZKJR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.025"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            334..1902
                     /gene="cydA"
                     /locus_tag="ACHOWS_001383"
     CDS             334..1902
                     /gene="cydA"
                     /locus_tag="ACHOWS_001383"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFQ0046994.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1918..3057
                     /gene="cydB"
                     /locus_tag="ACHOWS_001384"
     CDS             1918..3057
                     /gene="cydB"
                     /locus_tag="ACHOWS_001384"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFQ0046995.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFHLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3072..3185
                     /gene="cydX"
                     /locus_tag="ACHOWS_001385"
     CDS             3072..3185
                     /gene="cydX"
                     /locus_tag="ACHOWS_001385"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFQ0046996.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3185..3478
                     /gene="ybgE"
                     /locus_tag="ACHOWS_001386"
     CDS             3185..3478
                     /gene="ybgE"
                     /locus_tag="ACHOWS_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFQ0046997.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3628..4032
                     /gene="ybgC"
                     /locus_tag="ACHOWS_001387"
     CDS             3628..4032
                     /gene="ybgC"
                     /locus_tag="ACHOWS_001387"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFQ0046998.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4029..4721
                     /gene="tolQ"
                     /locus_tag="ACHOWS_001388"
     CDS             4029..4721
                     /gene="tolQ"
                     /locus_tag="ACHOWS_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFQ0046999.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4725..5153
                     /gene="tolR"
                     /locus_tag="ACHOWS_001389"
     CDS             4725..5153
                     /gene="tolR"
                     /locus_tag="ACHOWS_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFQ0047000.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5218..6483
                     /gene="tolA"
                     /locus_tag="ACHOWS_001390"
     CDS             5218..6483
                     /gene="tolA"
                     /locus_tag="ACHOWS_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFQ0047001.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6616..7908
                     /gene="tolB"
                     /locus_tag="ACHOWS_001391"
     CDS             6616..7908
                     /gene="tolB"
                     /locus_tag="ACHOWS_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFQ0047002.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7943..8464
                     /gene="pal"
                     /locus_tag="ACHOWS_001392"
     CDS             7943..8464
                     /gene="pal"
                     /locus_tag="ACHOWS_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFQ0047003.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8474..9265
                     /gene="cpoB"
                     /locus_tag="ACHOWS_001393"
     CDS             8474..9265
                     /gene="cpoB"
                     /locus_tag="ACHOWS_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFQ0047004.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     EAIVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9430..9505
                     /locus_tag="ACHOWS_001394"
     tRNA            9430..9505
                     /locus_tag="ACHOWS_001394"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9463..9465,aa:Lys,seq:ttt)
     gene            9641..9716
                     /locus_tag="ACHOWS_001395"
     tRNA            9641..9716
                     /locus_tag="ACHOWS_001395"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9674..9676,aa:Val,seq:tac)
     gene            9719..9794
                     /locus_tag="ACHOWS_001396"
     tRNA            9719..9794
                     /locus_tag="ACHOWS_001396"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9752..9754,aa:Lys,seq:ttt)
     gene            9846..9921
                     /locus_tag="ACHOWS_001397"
     tRNA            9846..9921
                     /locus_tag="ACHOWS_001397"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9879..9881,aa:Val,seq:tac)
     gene            9925..10000
                     /locus_tag="ACHOWS_001398"
     tRNA            9925..10000
                     /locus_tag="ACHOWS_001398"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9958..9960,aa:Lys,seq:ttt)
     gene            10147..10222
                     /locus_tag="ACHOWS_001399"
     tRNA            10147..10222
                     /locus_tag="ACHOWS_001399"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10180..10182,aa:Lys,seq:ttt)
     gene            10256..10331
                     /locus_tag="ACHOWS_001400"
     tRNA            10256..10331
                     /locus_tag="ACHOWS_001400"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10289..10291,aa:Lys,seq:ttt)
     gene            10579..11622
                     /gene="nadA"
                     /locus_tag="ACHOWS_001401"
     CDS             10579..11622
                     /gene="nadA"
                     /locus_tag="ACHOWS_001401"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFQ0047005.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCSVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPV
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11660..12379
                     /gene="pnuC"
                     /locus_tag="ACHOWS_001402"
     CDS             11660..12379
                     /gene="pnuC"
                     /locus_tag="ACHOWS_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0047006.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQMFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12376..13317)
                     /gene="zitB"
                     /locus_tag="ACHOWS_001403"
     CDS             complement(12376..13317)
                     /gene="zitB"
                     /locus_tag="ACHOWS_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFQ0047007.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13431..13811)
                     /gene="ybgS"
                     /locus_tag="ACHOWS_001404"
     CDS             complement(13431..13811)
                     /gene="ybgS"
                     /locus_tag="ACHOWS_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFQ0047008.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14127..15179
                     /gene="aroG"
                     /locus_tag="ACHOWS_001405"
     CDS             14127..15179
                     /gene="aroG"
                     /locus_tag="ACHOWS_001405"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFQ0047009.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            complement(15327..16079)
                     /gene="gpmA"
                     /locus_tag="ACHOWS_001406"
     CDS             complement(15327..16079)
                     /gene="gpmA"
                     /locus_tag="ACHOWS_001406"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0047010.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16282..17322)
                     /gene="galM"
                     /locus_tag="ACHOWS_001407"
     CDS             complement(16282..17322)
                     /gene="galM"
                     /locus_tag="ACHOWS_001407"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFQ0047011.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPF
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYTDWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            complement(17316..18464)
                     /gene="galK"
                     /locus_tag="ACHOWS_001408"
     CDS             complement(17316..18464)
                     /gene="galK"
                     /locus_tag="ACHOWS_001408"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0047012.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDATIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18468..19514)
                     /gene="galT"
                     /locus_tag="ACHOWS_001409"
     CDS             complement(18468..19514)
                     /gene="galT"
                     /locus_tag="ACHOWS_001409"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFQ0047013.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19524..20540)
                     /gene="galE"
                     /locus_tag="ACHOWS_001410"
     CDS             complement(19524..20540)
                     /gene="galE"
                     /locus_tag="ACHOWS_001410"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0047014.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20801..22273)
                     /gene="modF"
                     /locus_tag="ACHOWS_001411"
     CDS             complement(20801..22273)
                     /gene="modF"
                     /locus_tag="ACHOWS_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFQ0047015.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            complement(22341..23129)
                     /gene="modE"
                     /locus_tag="ACHOWS_001412"
     CDS             complement(22341..23129)
                     /gene="modE"
                     /locus_tag="ACHOWS_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFQ0047016.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARNHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23258..23407
                     /gene="acrZ"
                     /locus_tag="ACHOWS_001413"
     CDS             23258..23407
                     /gene="acrZ"
                     /locus_tag="ACHOWS_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFQ0047017.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23573..24346
                     /gene="modA"
                     /locus_tag="ACHOWS_001414"
     CDS             23573..24346
                     /gene="modA"
                     /locus_tag="ACHOWS_001414"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0047018.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24346..25035
                     /gene="modB"
                     /locus_tag="ACHOWS_001415"
     CDS             24346..25035
                     /gene="modB"
                     /locus_tag="ACHOWS_001415"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFQ0047019.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25038..26096
                     /gene="modC"
                     /locus_tag="ACHOWS_001416"
     CDS             25038..26096
                     /gene="modC"
                     /locus_tag="ACHOWS_001416"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFQ0047020.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26097..26915)
                     /gene="ybhA"
                     /locus_tag="ACHOWS_001417"
     CDS             complement(26097..26915)
                     /gene="ybhA"
                     /locus_tag="ACHOWS_001417"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFQ0047021.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27070..28065
                     /gene="pgl"
                     /locus_tag="ACHOWS_001418"
     CDS             27070..28065
                     /gene="pgl"
                     /locus_tag="ACHOWS_001418"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFQ0047022.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSEDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28106..29059)
                     /gene="ybhD"
                     /locus_tag="ACHOWS_001419"
     CDS             complement(28106..29059)
                     /gene="ybhD"
                     /locus_tag="ACHOWS_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0047023.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29243..30295
                     /gene="ybhH"
                     /locus_tag="ACHOWS_001420"
     CDS             29243..30295
                     /gene="ybhH"
                     /locus_tag="ACHOWS_001420"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFQ0047024.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLASIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISCSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30371..31804
                     /gene="ybhI"
                     /locus_tag="ACHOWS_001421"
     CDS             30371..31804
                     /gene="ybhI"
                     /locus_tag="ACHOWS_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFQ0047025.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            31987..34248
                     /gene="ybhJ"
                     /locus_tag="ACHOWS_001422"
     CDS             31987..34248
                     /gene="ybhJ"
                     /locus_tag="ACHOWS_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFQ0047026.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34389..35672)
                     /gene="ybhC"
                     /locus_tag="ACHOWS_001423"
     CDS             complement(34389..35672)
                     /gene="ybhC"
                     /locus_tag="ACHOWS_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFQ0047027.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35824..36300)
                     /gene="ybhB"
                     /locus_tag="ACHOWS_001424"
     CDS             complement(35824..36300)
                     /gene="ybhB"
                     /locus_tag="ACHOWS_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFQ0047028.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNIHFHSLASASITAMFS"
     gene            complement(36359..37648)
                     /gene="bioA"
                     /locus_tag="ACHOWS_001425"
     CDS             complement(36359..37648)
                     /gene="bioA"
                     /locus_tag="ACHOWS_001425"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFQ0047029.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTVAVNRAVQDETFFCQ"
     gene            37735..38775
                     /gene="bioB"
                     /locus_tag="ACHOWS_001426"
     CDS             37735..38775
                     /gene="bioB"
                     /locus_tag="ACHOWS_001426"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFQ0047030.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLASARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            38772..39926
                     /gene="bioF"
                     /locus_tag="ACHOWS_001427"
     CDS             38772..39926
                     /gene="bioF"
                     /locus_tag="ACHOWS_001427"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFQ0047031.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDTRREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            39913..40668
                     /gene="bioC"
                     /locus_tag="ACHOWS_001428"
     CDS             39913..40668
                     /gene="bioC"
                     /locus_tag="ACHOWS_001428"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFQ0047032.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            40661..41338
                     /gene="bioD"
                     /locus_tag="ACHOWS_001429"
     CDS             40661..41338
                     /gene="bioD"
                     /locus_tag="ACHOWS_001429"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFQ0047033.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
BASE COUNT         9891 a        10240 c        11053 g        10168 t
ORIGIN      
        1 taattcttga aaaaaatctg caaaacacga taaattgtcg tagatcaaat tggtgagatc
       61 gtgacaattt cgctataaat tgatcactgt cgaaaaatgc aaatttgctc aataaaaacc
      121 tgtttattgt aaggattttg cggcgtaata taattacggg atcaatttgg tttattcatt
      181 aacacatttg taactttttt atcattgtct tttcctcttt tgatgaaatg gcaacgatag
      241 aggcgagcaa ataattttgt attggggcat gcgtgtgacc ctttctaacg gggttcactc
      301 tcggagtctt catgcgatga gcaaggagtc atgatgttag atatagtcga actgtcgcgc
      361 ttacagtttg ccttgaccgc gatgtaccac ttcctttttg tgccactgac gctcggtatg
      421 gcgttcctgc tggccattat ggaaacggtc tacgtccttt ccggcaaaca gatttataaa
      481 gatatgacca agttctgggg caagttgttt ggtatcaact tcgctctggg tgtggctacc
      541 ggtctgacca tggagttcca gttcgggact aactggtctt actattccca ctatgtaggg
      601 gatatcttcg gtgcgccgct ggcaatcgaa ggtctgatgg ccttcttcct cgaatccacc
      661 tttgtaggtc tgttcttctt cggttgggat cgtctgggta aagttcagca tatgtgtgtc
      721 acctggctgg tggcgctcgg ttcaaacctg tccgcactgt ggattctggt tgcgaacggc
      781 tggatgcaaa acccaatcgc gtccgatttc aactttgaaa ctatgcgtat ggagatggtg
      841 agcttctccg agctggtgct taacccggtt gctcaggtga aattcgttca cactgtagcg
      901 tctggttatg tgactggcgc gatgttcatc ctcggtatca gcgcatggta tatgctgaaa
      961 ggtcgtgact tcgccttcgc taaacgctct tttgctatcg ctgccagctt cggtatggct
     1021 gccgttctgt ctgttattgt tctgggtgat gaatctggtt acgaaatggg cgacgtgcag
     1081 aaaaccaaac tggctgctat tgaagccgag tgggaaacgc aacctgcgcc tgctgccttt
     1141 actctgttcg gcattcctga tcaggaagag gagacgaaca aatttgcgat ccagatccct
     1201 tacgcgctgg gcatcattgc aacgcgttcc gtggatactc cagttatcgg cctgaaagaa
     1261 ctgatggtgc agcatgaaga acgcattcgt aacgggatga aggcgtactc tctgctcgag
     1321 caactgcgtt ctggttctac cgaccaggcg gttcgtgacc agttcaatag catgaagaaa
     1381 gacctcggtt acggtctgct gctgaaacgc tatacgccaa acgtggctga tgcgactgaa
     1441 gcgcagattc aacaggcaac caaagactcc attccgcgtg tagcgccgct gtacttcgcg
     1501 ttccgtatca tggtggcgtg tggcttcctg ctgctggcaa tcatcgcgct ctctttctgg
     1561 agtgtcatcc gcaaccgcat tggcgagaaa aaatggctgc tgcgcgccgc gctgtacggt
     1621 attccgctgc cgtggattgc tgtagaagcg ggctggttcg tggctgaata tggtcgccaa
     1681 ccgtgggcta tcggtgaagt gctgccgaca gctgtggcga actcgtcact gaccgcaggc
     1741 gatctcatct tctcaatggt gctgatttgc ggcctgtata ccctgttcct ggtggcagaa
     1801 ttgttcttaa tgttcaaatt tgcacgcctc ggcccaagca gcctgaaaac cggtcgctat
     1861 cactttgagc agtcttccac gactactcag ccggcacgct aagacaggag tcgtcaaatg
     1921 atcgattatg aagtattgcg ttttatctgg tggctgctgg ttggcgttct gctgattggt
     1981 tttgcagtca ctgacggttt cgacatgggg gtgggcatgc tcactcgttt cctcggtcgt
     2041 aacgacaccg agcgtcgaat tatgattaac tccattgcac cacactggga cggtaaccag
     2101 gtatggctta tcaccgcggg cggcgcactc tttgctgcct ggccgatggt ctatgccgct
     2161 gcgttctccg gcttctatgt ggcgatgatc ctcgtgctgg cgtctttgtt cttccgtccg
     2221 gtcggttttg actaccgctc caagattgaa gaaacccgct ggcgtaacat gtgggactgg
     2281 ggcatcttca ttggtagctt cgttccgccg ttggtgattg gtgtagcgtt cggtaacctg
     2341 ttgcagggcg taccgttcaa cgttgatgaa tatctgcgtc tgtactacac cggtaacttc
     2401 ttccacctgc ttaacccgtt cggcctgctg gcaggcgtgg tgagcgtagg gatgatcatt
     2461 actcagggcg caacctatct gcaaatgcgt accgtgggcg aactgcacct gcgtacccgt
     2521 gcaacggctc aggtggcggc actggtgaca ctggtctgtt tcgcactggc tggcgtatgg
     2581 gtgatgtacg gtatcgatgg ttatgtcgtg aaatcgacaa tggaccatta cgcagcctct
     2641 aacccactga ataaagaagt ggttcgtgaa gctggcgcat ggctggttaa cttcaacaac
     2701 acgccaattc tgtgggctat tccggcactg ggtgtggttc tgccgctgct gaccatcctg
     2761 actgcacgta tggataaagc ggcgtgggcg tttgtgttct cctccctgac gctggcctgc
     2821 atcatcctga cagccggtat cgcaatgttc ccgtttgtga tgccgtccag caccatgatg
     2881 aacgcaagtc tgacaatgtg ggatgcaact tccagccagc tgacgcttaa cgtcatgacc
     2941 tgggttgcgg tggttctggt accgatcatt ctgctctaca ccgcctggtg ttactggaaa
     3001 atgttcggtc gtatcaccaa agaagatatt gaacgtaaca cccactctct gtactaagta
     3061 aggagctaaa aatgtggtat ttcgcatgga ttctgggaac gcttcttgcc tgttcgtttg
     3121 gggtaatcac cgcgctggcg cttgagcacg tcgaatcagg caaagccggt caagaagaca
     3181 tctgatgagt aagattatcg caaccttgta tgcggtaatg gacaagcgcc ccctgcgggc
     3241 gctttccttc gtgatggcgc ttctgttagc aggatgtatg ttttgggacc catcacgttt
     3301 cgccgcgaag accagtgaac tggaaatctg gcatggttta ttgctgatgt gggccgtctg
     3361 tgctggtgtg attcacggcg tgggctttcg tccgcagaag gttctttggc aagggatttt
     3421 ttgcccattg cttgccgata ttgttctcat tgtcgggctg attttcttct tcttttaaat
     3481 cagaattctc tttaaaaaat tatgggccgc tccaggccca taaattttta cgctccctta
     3541 acttgccctc attcccaaac ctcaatcgcg cgcgtatagt agcagcgttt aaaagctcta
     3601 acttttgttg cattaccggg atgtaaagtg aatacaacgc tgtttcgatg gccggttcgc
     3661 gtctactatg aagataccga tgccggtggt gtggtgtacc acgccagtta cgtcgctttt
     3721 tatgaaagag cacgcacaga gatgctgcgt catcatcact tcagtcaaca ggcgctgatg
     3781 gctgaacgcg ttgcctttgt ggtacgtaaa atgacggtgg aatattacgc acctgcgcgg
     3841 ctcgacgata tgctcgaaat acagactgaa ataacatcaa tgcgtggcac ctctttggtt
     3901 ttcacgcaac gtattgtcaa cgccgagaat actttgctga atgaagcaga ggttctggtt
     3961 gtttgcgttg acccactcaa aatgaagcct cgtgcgcttc ccaagtctat tgtcgcggag
     4021 tttaagcagt gactgacatg aatatccttg atttgttcct gaaggctagc cttctggtta
     4081 aacttatcat gttgattttg attggttttt caatcgcatc ctgggccatt attatccagc
     4141 ggacccgtat tcttaacgca gcggcgcgtg aagccgaagc gtttgaagat aaattctggt
     4201 ctggaatcga actctctcgc ctctatcaag agagtcaggg gaaacgggat aatctgactg
     4261 gttcggaaca aatcttttac agcgggttca aagagtttgt gcgcctgcat cgtgccaata
     4321 gccatgcgcc ggaagccgta gtggaagggg cgtcgcgtgc tatgcgtatc tccatgaacc
     4381 gtgaacttga aaatctggaa acgcacattc cgttcctcgg tacggttggc tccatcagcc
     4441 cgtatattgg tctgtttggt acggtctggg ggatcatgca cgcctttatc gccctcgggg
     4501 cggtaaaaca agcaacactg caaatggttg cgcccggtat cgcagaagcg ttgattgcga
     4561 cggcaattgg tctgttcgcc gcaattccgg cggtaatggc ttataaccgt ctcaaccagc
     4621 gcgtaaacaa actggaactg aattacgaca actttatgga agagtttacc gcgattctgc
     4681 accgccaggc gtttaccgtt agcgagagca acaaggggta agccatggcc agagcgcgtg
     4741 gacgaggtcg tcgcgatctc aagtccgaaa tcaacattgt accgttgctg gacgtactgc
     4801 tggtgctgtt gctgatcttt atggcgacag cgcccatcat cacccagagc gtggaggtcg
     4861 atctgccaga cgctactgaa tcacaggcgg tgagcagtaa cgataatccg ccagtgattg
     4921 ttgaagtgtc tggtattggt cagtacaccg tggtggttga gaaagatcgc ctggagcgtt
     4981 taccaccaga gcaggtggtg gcggaagtgt ccagccgttt caaggccaac ccgaaaacgg
     5041 tctttctgat cggtggcgca aaagatgtgc cttacgatga aataattaaa gcactgaact
     5101 tgttacatag tgcgggtgtg aaatcggttg gtttaatgac gcagcctatc taaacatctg
     5161 cgtttccctt gcttgaaaga gagcgggtaa caggcgaaca gtttttggaa accgagagtg
     5221 tcaaaggcaa ccgaacaaaa cgacaagctc aagcgggcga taattatttc agcagtgctg
     5281 catgtcatct tatttgcggc gctgatctgg agttcgttcg atgagaatat agaagcttca
     5341 gccggaggcg gcggtggttc atccatcgac gctgtcatgg ttgattcagg tgcggtagtt
     5401 gagcagtaca aacgcatgca aagccaggaa tcaagcgcga agcgttctga tgagcagcgc
     5461 aagatgaagg aacagcaggc tgcggaagag ctgcgtgaga aacaagcggc tgaacaggaa
     5521 cgcctgaagc aacttgagaa agagcggtta gctgctcagg agcagaaaaa gcaggctgaa
     5581 gaagccgcaa aacaggccga gttaaagcag aagcaagcgg aagaagcggc agcgaaagcg
     5641 gcggcagatg ctaaagcgaa ggccgaagcg gatgctaaag ctgcggaaga agctgcgaag
     5701 aaagcggctg cagacgcgaa gaaaaaagca gaagcagaag ccgccaaagc cgcagccgaa
     5761 gcgcagaaaa aagccgaggc agccgcggcg gcactgaaga agaaagcgga agcggcagaa
     5821 gcagctgcag ctgaagcaag aaagaaagcg gcaactgaag ctgctgaaaa agccaaagca
     5881 gaagctgaga agaaagcggc tgctgaaaag gctgcagctg ataagaaagc ggcagcagag
     5941 aaagctgcag ccgacaaaaa agcagcagaa aaagcggctg ctgaaaaggc agcagctgat
     6001 aagaaagcag cggcagaaaa agccgccgca gacaaaaaag cggcagctgc aaaagcagca
     6061 gctgaaaaag ccgctgcagc aaaagctgcc gcagaggcag atgatatttt cggtgagcta
     6121 agctctggta agaatgcacc gaaaacgggg ggaggggcga aagggaacaa tgcttcgcca
     6181 gccgggagtg gtaatactaa aaacaatggc gcatcagggg ccgatatcaa taactatgcc
     6241 gggcagatta aatctgctat cgaaagtaag ttctatgacg catcgtccta tgcaggcaaa
     6301 acctgtacgc tgcgcataaa actggctccc gatggtatgt tactggatat caaacctgaa
     6361 ggtggcgatc ccgcactttg tcaggctgcg ttggcagcag ctaaacttgc gaagatcccg
     6421 aaaccaccaa gccaggcagt atatgaagtg ttcaaaaacg cgccattgga cttcaaaccg
     6481 taatcgcgat gttgactgtt ccgacggtca acatcaggca ccggttgcca cggggttctg
     6541 gtagttttgt gtattttagt ttgttaacat tctgctaaat tatcgtgggc catcggtcca
     6601 gataagggag atatgatgaa gcaggcatta cgagtagcat ttggttttct catactgtgg
     6661 gcatcagttc tgcatgctga agtccgcatt gtgatcgaca gcggtgtaga ttccggtcgt
     6721 cctattggag ttgttccttt ccagtgggcg ggccctggtg cggcacctga agatattggc
     6781 ggcatcgttg ctgccgactt gcgtaacagc ggtaaattta atccgttaga tcgcgctcgt
     6841 ctgccacagc agccgggtag tgcgcaggaa gtacaaccag ctgcatggtc cgcgctgggt
     6901 attgacgctg tagttgtcgg tcaggtcact ccgaatccgg atggttctta caatgttgct
     6961 tatcaacttg ttgacactgg cggcgcaccg ggtactgtac ttgctcagaa ctcgtacaaa
     7021 gtgaacaagc agtggctgcg ttatgctggt cataccgcca gtgatgaagt gtttgaaaaa
     7081 ctgaccggca ttaaaggtgc gttccgtacc cgtattgcct acgttgttca gaccaacggc
     7141 ggtcagttcc cgtatgaact gcgcgtatct gactatgacg gttacaacca gtttgtcgtt
     7201 caccgttcac cgcagccgct gatgtctccg gcgtggtcac cagacggttc taaactggct
     7261 tatgtgacct tcgaaagcgg tcgttccgcg ctggtcatcc aaacgctggc aaatggcgct
     7321 gtacgtcagg tggcttcatt cccgcgtcac aacggtgcac ctgcattctc gccagacggc
     7381 agcaaactgg cattcgcctt gtcgaaaacc ggtagtctga acctgtacgt aatggatttg
     7441 gcttctggtc agatccgcca ggtgacggat ggtcgcagta acaataccga accgacctgg
     7501 ttcccggaca gccagaacct ggcatttact tctgaccagg ccggtcgtcc acaggtttat
     7561 aaagtgaata tcaacggcgg tgcgccacaa cgtattacct gggaaggttc gcagaaccag
     7621 gatgcggatg tcagcagcga cggtaaattt atggtaatgg tcagctccaa tggtgggcag
     7681 cagcacattg ccaaacaaga tctggcaacg ggaggcgtac aagttctgtc gtccacgttc
     7741 ctggatgaaa cgccaagtct ggcacctaac ggcactatgg taatctacag ctcttctcag
     7801 gggatgggat ccgtgctgaa tttggtttct acagatgggc gtttcaaagc gcgtcttccg
     7861 gcaactgatg gacaggtcaa attccctgcc tggtcgccgt atctgtgata ataattaatt
     7921 gaatagtaaa ggaatcattg aaatgcaact gaacaaagtg ctgaaagggc tgatgattgc
     7981 tctgcctgtt atggcaattg cggcatgttc ttccaataag aacgccagca atgacggcag
     8041 cgaaggcatg ctgggtgccg gcactggtat ggatgcgaac ggcggcaacg gcaacatgtc
     8101 ttccgaagag caggctcgtc tgcaaatgca acagctgcag cagaacaaca tcgtttactt
     8161 cgatctggac aagtacgata tccgttctga cttcgctcaa atgctggatg cacatgcaaa
     8221 cttcctgcgt agcaacccgt cttacaaagt caccgtagaa ggtcacgcgg acgaacgtgg
     8281 tactccggaa tacaacatct ccctgggtga acgtcgtgcg aacgccgtta agatgtacct
     8341 gcagggtaaa ggcgtttctg cagaccagat ctccatcgtt tcttacggta aagaaaaacc
     8401 tgcagtactg ggtcatgacg aagcggcata ctccaaaaac cgtcgtgcgg tactggttta
     8461 ctaagagaat tgcatgagca gtaacttcag acatcaacta ttgagtctgt cgttactggt
     8521 tggtatagcg gccccctggg ccgcttttgc tcaggcacca atcagtagtg tcggctcagg
     8581 ctcggtcgaa gaccgcgtca ctcaacttga gcgtatttca aatgctcaca gccagctttt
     8641 aacccaactc cagcaacaac tctctgataa tcaatccgat attgattccc tgcgcggtca
     8701 gattcaggaa aatcagtatc aactgaatca ggtcgtggag cggcaaaagc agatcctgtt
     8761 gcagatcgat agcctcagca gcggtggtgc agcggcgcaa tcaaccagcg gtgatcaaag
     8821 cggtgcggcg gcatcaacga cgccgacagc tgatgctggt actgcgaatg ctggcgcgcc
     8881 ggtgaaaagc ggcgatgcaa acacggatta caatgcagca attgcgctgg tgcaggataa
     8941 atcccgccag gatgaagcaa tagtggcatt tcagaatttc atcaaaaatt accctgattc
     9001 aacttacctg ccaaacgcca attattggct gggtcagtta aactacaaca agggtaaaaa
     9061 agatgatgcg gcgtattatt ttgcttcggt agtgaaaaac tatccgaagt caccgaaggc
     9121 tgcagatgcg atgtttaaag tcggcgtcat catgcaggac aaaggtgaca ccgcaaaagc
     9181 gaaagccgta taccagcagg ttatcagtaa ataccctggt actgatggcg ctaaacaggc
     9241 gcaaaaacgt ctgaacgcga tgtaatgcat aacacacgac cagaagtcgc attatttctg
     9301 gtcgtgtcgt gcgaatcata agcagttgag tgatctgcat cgaaattttt gttgcgctca
     9361 agtctgaaat cagtaatata tgccgccgtt gccacgggat atcaaacaaa ccgaaagcaa
     9421 cgaaaaagtg ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca
     9481 ggttcgaatc ctgcacgacc caccaatcgc taaggtggaa gcggtagtaa aacgtgaagg
     9541 ataacgttgc gtcagcaacg gcccgaaggg cgagacgaag tcgagtcatc ctgcacgacc
     9601 caccactaac ctagttagtt gtagtatcca gcgtagtatc gggtgattag ctcagctggg
     9661 agagcacctc ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaccgg
     9721 gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc
     9781 tgcacgaccc accactaatt acggtgggtt cggtggaagt agtttgtagt attcagcgca
     9841 gtatcgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
     9901 cgatcccgtc atcacccacc actcgggtcg ttagctcagt tggtagagca gttgactttt
     9961 aatcaattgg tcgcaggttc gaatcctgca cgacccacca gttttaacat caaactcaga
    10021 tgttaagcgt gaaggataac gttgcgccag caacggcccg tagggcgagc gaagcgagtc
    10081 atcctgcacg acccaccaat tttaaagatt ggttccgagt agaaatcttt caggtaacac
    10141 ccgtatgggt cgttagctca gttggtagag cagttgactt ttaatcaatt ggtcgcaggt
    10201 tcgaatcctg cacgacccac catcctgaat gattaaagca gcataatccc gcaaggggtc
    10261 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
    10321 acgacccacc aatgtaaaaa agcgccctaa aggcgctttt ttgctattca ggcatcctca
    10381 atttcacttt gtaacctgat gacatcttca gagcttactt tgcaagcaac tctacgtcgg
    10441 tggaattagg cgtaattcga gtgacttttc cccaccattc gactatcttg tttagcatat
    10501 aaaacaaatt tcaccgataa cagcgaatat tacgctcatg tcggttttaa cgttaagcct
    10561 gtaaaacgag atggtaagat gagcgtgatg tttgatccag acacggcgat ttatcctttt
    10621 cccccgaagc cgacgccgtt aagcattgat gaaaaagcgt attaccgcga gaagataaaa
    10681 cgtctgctaa aagaacgtaa tgcggtgatg gttgcccact actataccga tcccgaaatt
    10741 caacaactgg cagaagaaac cggtggctgt atttctgatt ctctggaaat ggcgcgcttc
    10801 ggtgcaaagc atcccgcttc tacgttgtta gtcgctgggg tgagatttat gggagaaacc
    10861 gccaaaattc tcagtccgga aaaaacaatt ctgatgccga cacttcaggc tgaatgttca
    10921 ctggatctcg gctgctctgt tgaagaattt aacgcatttt gcgatgccca tcccgatcgt
    10981 actgtcgtcg tctacgccaa cacttctgct gcggtaaaag cgcgcgcaga ttgggtggta
    11041 acttcaagca ttgccgtcga acttattgat catctggata gtttgggtga aaaaatcatc
    11101 tgggcacccg acaaacatct ggggcgttac gtgcaaaaac agacgggcgc agacatatta
    11161 tgctggcagg gtgcctgcat tgtgcatgat gagtttaaga ctcaggcgtt aacccgcttg
    11221 caagaagaat acccggatgc tgccatactg gtgcatccag aatcaccgca agctattgta
    11281 gagatggcgg atgcggtcgg ttccaccagt caactgatcg ctgctgcgaa aacattgcca
    11341 catcagaggc ttattgtggc aaccgatcgg ggtattttct acaaaatgca gcaggcggtg
    11401 ccagttaaag agttactgga agcaccaacc gcaggtgagg gtgcaacctg ccgcagctgc
    11461 gcgcattgtc cgtggatggc catgaatggc cttcaggcca tcgcagaggc attagaacag
    11521 gaaggaagca atcacgaggt tcatgttgat gaaaggctgc gagagagggc gctggtgccg
    11581 ctcaatcgta tgctggattt tgcggctaca ctacgtggat aacgaataat aaggcgtaac
    11641 gttacgcttt gggggaaaga tggatttttt tagtgtgcag aatatcctgg tacatatacc
    11701 aataggtgca ggcggttatg atctctcatg gatcgaagcg gtaggcacga tcgccgggtt
    11761 gctgtgtatt ggccttgcca gtctggagaa gatcagcaac tacttctttg gcctgatcaa
    11821 cgtcaccttg tttggcatta ttttctttca gattcagctg tatgccagcc tgctattaca
    11881 gatgtttttc tttgccgcga atatttacgg ctggtatgcg tggtcgcgac aaaccagtca
    11941 gaacgaggcg gagttgaaaa ttcgctggtt gccattgccg aaggcgctaa gctggttggc
    12001 ggtttgcgtt gtttcgattg gtctgatgac ggtatttatc aatccggtgt ttgcattttt
    12061 gacccgcgtg gcagtcatga tcatgcaagc attaggatta caggttgcga tgcctgaact
    12121 acagccggac gctttcccgt tctgggattc atgcatgatg gtgttatcta tcgtggcaat
    12181 gattctgatg acgcgtaagt atgtggaaaa ctggctgttg tgggtgatta ttaacgtgat
    12241 tagcgtcgtt atttttgcac ttcagggcgt ttacgccatg tctctggagt acatcatcct
    12301 gacctttatt gccctcaacg gcagccggat gtggatcaac agcgcacgcg aaagaggttc
    12361 acgcgcgctg tcccattaat ggtgatgatg tgaatggcca gacacccctt cattcaggtg
    12421 gcagtctggc ccatgacaag gttgatattc catctgaata gtggcgtgct caatctgata
    12481 gtgatccatc aggtagtgtt ggatctgatc taacaaggca tcgtgatcgt gtggcgggat
    12541 cacctgcaca tgcagcgtca tcaccggctt ctcgcctacc atccatacat gcacatggtg
    12601 cacattgcgg acttccggga tttcccggca catacgacgc ttcagttcgg cgatatccag
    12661 cgataccggt gcaccttcaa gtaattcatt cacgctatct ttcaacagtc gccacgcgct
    12721 acgcagaacc agaagcgaca ccagtattga gagaatgggg tcagcaggtg tccagccggt
    12781 ccagataata atcaacgcgg caataatagc cccaaccgaa cccagcaggt ccccgagcac
    12841 atgcagtgcc gctgctcgca cgttgaggtt tttctcttcg ctgccgtgat gaagtaacca
    12901 gaaagaaagt atatttgcca gcagcccagc cacggcaatt gccatcatca tgccgccctc
    12961 gaccggacgc ggagtgcgga agcgttctat cgcctcccag acaatcaaaa tagtaatcac
    13021 caccagggcg atggcattca caaacgccgc gagcgtggtc aatcttagcc aaccgaaagt
    13081 gtggcgaatg gtgggaggac gacgggaaaa ttgcactgcg agtagggcga aaagcagggc
    13141 ggcagtatcg gtcaacatat gacccgcatc ggccagcaat gccagagaac cggaaagaaa
    13201 accaccaatg acttctacga gcataaaccc ggcagtcacg ccgaaagcat acaacaagcg
    13261 acgagcatta ttatcttctg gcaggtgtga ggaagtgtgt gagtgtgagt gcgccatgac
    13321 gagtcatccc ttaattattc tcacttaact ttatgacatc atactgcttt tagaagggaa
    13381 aaattaaaag ggagagactc cgctctccca ttattggcta tttcgcaggg ttactgcgtg
    13441 gtaccgtcgg ttttggtatc gacatcattg ttgatgccat caccggtttg gactttttta
    13501 ttgatatccg ggcagcgacc atctttgcac atggtgttct tgtgctcttc atctttggtc
    13561 atgccgtcat tgttcattga agaaccatcc ggatgcagca ttgtgccgcc agaaccggta
    13621 tttaccccgt tattgtcgac gttatttggc gcgacatttt cacgggcatc aggggctacc
    13681 tggcccgcat cagctgcggc gtttgcctgg ccgttattgc tttgcgctcc gctatcggca
    13741 gccagtgcgg caccgctggc aaggcttaga gtggcagtca aaaatagtgt ggccagtttt
    13801 gtcattttca taggatgctc ctgttatggt cgttatgtcg gataacctct tccaacagtg
    13861 catttgcagg tgaatataag gcaatggttt aagatttcag ccaggcaaag atacccagca
    13921 gagaatcttg aaataattaa caaacaaagg agttacagtt agaaattgta ggagagatct
    13981 cgtttttcgc gacaatctgg cgattttctt gctaattcca ggattaatcc gttcatagtg
    14041 taaaaccccg tttacacatt ctgacggaag atatagattg gaagtattgc attcactaag
    14101 ataagtatgg caacactgga acagacatga attatcagaa cgacgattta cgcatcaaag
    14161 aaatcaaaga gttacttcct ccagtcgcat tgctggaaaa attccccgct actgaaaatg
    14221 ccgcgaatac ggtcgcccat gcccgaaaag cgatccataa gatcctgaaa ggtaatgatg
    14281 atcgcctgtt ggtggtgatt ggtccgtgct caattcatga tcctgtcgcg gcgaaagagt
    14341 atgccactcg cttgctgacg ctgcgtgaag agctgcaaga tgagctggaa atcgtgatgc
    14401 gcgtctattt tgaaaagccg cgtaccacgg tgggctggaa agggctgatt aacgatccgc
    14461 atatggataa cagcttccag atcaacgacg gtctgcgtat tgcccgtaaa ttgctgcttg
    14521 atattaacga cagcggtctg ccagcggcgg gtgaattcct ggatatgatc accccacaat
    14581 atctcgctga cctgatgagc tggggcgcaa ttggcgcacg taccaccgaa tcgcaggtgc
    14641 accgcgaact ggcatcaggg ctttcttgtc cggtcggctt caaaaatggc accgacggta
    14701 cgattaaagt ggctatcgat gccattaatg ccgccggtgc gccgcactgc ttcctgtccg
    14761 taacgaaatg ggggcattcg gcgattgtga ataccagcgg taacggcgat tgccatatca
    14821 ttctgcgcgg cggtaaagag cctaactaca gcgcgaagca cgttgctgaa gtgaaagaag
    14881 ggctgaacaa agcaggcctg ccagcacagg tgatgatcga tttcagccat gctaactcgt
    14941 caaaacaatt caaaaagcag atggatgttt gtgctgacgt ttgccagcag attgccggtg
    15001 gcgaaaaggc tattattggc gtgatggtgg aaagccatct ggtggaaggc aatcagagcc
    15061 tcgagagtgg ggaaccgctg gcctatggta agagcatcac cgatgcctgc attggctggg
    15121 atgataccga tgctctgtta cgtcaactgg cgaatgcagt aaaagcgcgt cgcgggtaat
    15181 ttatagtcga atcgccatga ctgtacgttt gcagccctga taagacgcgc agcgtagcat
    15241 caggcaatgt gctccattgt tagcaacgac aaacaaaaaa gccgactcac ttgcagtcgg
    15301 ctttctcatt tttaagcgaa tgacgtttac ttcgctttac cctggttcgc aacggccgct
    15361 gctttcgccg cgatctcgtc agcattaccc agataatagc gtttcaacgg tttgaaattc
    15421 tcgtcgaact catacaccag tggcacacca gtcgggatat tcagctcaag aatctcttct
    15481 tcgctcatgt tatcaagata tttcaccagc gcacgtaacg agttaccgtg tgcagcgatg
    15541 attacgcgct caccgctctt catacgcggc agaatggttt cattccagta agggatcacg
    15601 cggtcaatgg tcagcgccag gctttccgtc agtggcagtt ctttctcgct cagcttcgcg
    15661 taacgtggat cgtgacccgg ataacgctca tcatctttag tcagttccgg cggagtcact
    15721 gcaaaaccac gacgccactg tttcacctgc tcgtcgccat acttttcagc ggtttctgct
    15781 ttgttcagac cctgcaacgc accgtagtga cgttcgttca gtttccagga tttctcaacg
    15841 ggcagccatg cctgatccag ttcgtccagc acattccaca gggtatggat agcgcgtttc
    15901 agcacagagg tgtaagcaaa gtcaaagctg taaccttcct ctttcagcag cttacctgct
    15961 gcttttgctt cgcttacgcc tttctcagac agatccacgt cgtaccaacc ggtgaaacgg
    16021 ttttctttgt tccactgact ttcgccatga cgaaccagaa ccagcttagt tacagccata
    16081 tacttactcc tcaaatcatc ttttaatgat aataattctc attatattgc cgtgacgaag
    16141 caacagcaat gcttacgcat aaccatagcg aaaatagtgt cgcagtgtaa ggttgttgtg
    16201 aatattgagt tgcaaatatg tcggtgtttg ctggtgattt gaacaatatg aagataaagc
    16261 cctcatgacg agggcttaac attactgagc aataaactga tattccgtca ggctggaata
    16321 ctcttcgcca ggacgcagga aacagtccgg ttgcggccat tcagggtggt tcgggctgtc
    16381 cggcagaaac tcgctttcca acgccagccc ttgccagtcg gtgtaaggtt cggttccccg
    16441 cgacggtgtg ccgccgaggt agttgccgga gtagaattgc agagccggag cggtggtgta
    16501 gaccttcagc tgcaattttt catctgctga ccagacatgc gccgccactt tcttgccatc
    16561 gcctttggcc tgtaacaaga acgcgtgatc gtaacctttc actttgcgct gatcgtcgtc
    16621 ggcaagaaac tcactggcga tgattttggc gctgcggaaa tcaaaagacg ttccggcgac
    16681 agatttcagg ccgtcgtggg gaatgccgcc ttcatcaacc ggcagatatt cgtccgccag
    16741 aatctgcaac ttgtgattgc gcacgtcaga ctgctcgccg tcaagattga aatagacgtg
    16801 attagtcata ttcaccgggc aaggtttatc aactgtggcg cgataagtaa tagagatacg
    16861 gttatcgtcg gtcagacgat attgtaccgt cgctccgaga ttacccggga agccctgatc
    16921 gccatcatct gaactcaggg caaacagcac ctgacgatcg ttctggttca caatctgcca
    16981 gcgacgtttg tcgaaccctt ccgggccgcc gtgcagctgg ttaacgccct gacttggtga
    17041 aagcgtcacg gtttcaccgt caaaggtata acggctattg gcgatacggt tggcataacg
    17101 accaatagag gcccccagaa atgcggcctg atcctgatag cattccgggc tggcacagcc
    17161 gagcagcgcc tcgcggacgc tgccatcgga aaacggaata cgggcggaaa gtaaagtcgc
    17221 accccagtcc atcagcgtga ctaccatccc tgcgttgtta cgcaaagtta acagtcggta
    17281 cggctgacca tcgggtgcca gtgcgggagt ttcgttcagc actgtcctgc tccttgtgat
    17341 ggtttacaaa cgtaaaaagt ctctttaata cctgtttttg cttcatattg ttcagcgaca
    17401 gcttgctgta cggcaggcac cagctcttcc gggatcagcg cgacgataca gccgccaaat
    17461 ccgccgccgg tcatgcgtac gccacctttg tcgccaatca cagctttgac gatttctacc
    17521 agagtgtcaa tttgcggcac ggtgatttcg aaatcatcgc gcatagaggc atgagactcc
    17581 gccatcaact cgcccatacg tttcaggtcg ccttgctcca gtgcgctggc agcttcaacg
    17641 gtgcgggcgt tttcagtcag gatatgacgc acgcgtttcg ccacgattgg gtccagttca
    17701 tgcgcaacag cgttgaactc ttcaatggtg acatcgcgca gcgctggctg ctggaagaaa
    17761 cgcgcaccag tttcgcactg ttcacgacgg gtgttgtatt cgctgccaac cagggtacgt
    17821 ttgaagttac tgttaatgat gacgacagcc acacctttcg gcatggaaac tgctttagtc
    17881 cccagtgagc ggcagtcaat cagcaaggca tgatctttct tgccgagtgc ggaaattagc
    17941 tgatccatga tcccgcagtt acagccaaca aactggtttt ctgcttcctg accgttaagc
    18001 gcgatttgtg cgccgtccag cggcaaatga taaagctgct gcaatacggt tccgaccgcg
    18061 acttccagtg aagcggaaga acttaacccg gcaccctgcg gcacattgcc gctgatcacc
    18121 atgtccacgc cgccgaagct gttgttacgc agttgcagat gtttcaccac gccacgaacg
    18181 tagttagccc attgatagtt ttcatgcgcg acaatggtcg catcgaggga aaactcgtcg
    18241 agctgatttt cataatcggc tgccatcacg cgaactttac ggtcatcgcg tggtgcacaa
    18301 ctgatcacgg tttgataatc aatcgcgcag ggcagaacga aaccgtcgtt gtagtcggtg
    18361 tgttcaccaa tcaaattcac gcggccaggc gcctgaatgg tgtgagtggc agggtagcca
    18421 aatgcgttgg caaacagaga ttgtgttttt tctttcagac tcatttctta cactccggat
    18481 tcgcgaaaat ggatatcgct gactgcgcgc aaacgctctg cggcctgttc tgcggtcagg
    18541 tctcgctggg tttctgccag catttcataa ccaaccataa atttacgtac ggtggcagag
    18601 cgcaacagag gcggataaaa gtgcgcgtgc agctgccagt gttgattctc ttcgccatta
    18661 aatggcgcgc cgtgccagcc catagagtag gggaaggagc actggaagag gttgtcataa
    18721 cgactggtca gctttttcaa cgccagtgcc aaatcgctgc gctgggcgtc ggtcaaatcg
    18781 gtgatccgta aaacgtgggc tttgggcagc agtagcgttt cgaacggcca ggcagcccag
    18841 taaggtacaa cggctaacca gtgttcggtt tcgacaacgg tacggctacc gtctgccagc
    18901 tcgcgctgaa cataatccac cagcattggc gatttctgtt cggcgaaata ttctttttgc
    18961 agacggtctt cgcgctcagc ttcgttaggc aggaagctat ttgcccaaat ctgtccgtgc
    19021 ggatgcgggt tagagcagcc catcgccgcg cctttgtttt caaagacctg cacccatggg
    19081 tacgttttcc ccagttctgc ggtttgctcc tgccaggttt tgacgatttc cgtcaatgcc
    19141 gcaacgctga gttctggcaa cgttttgctg tgatccggtg aaaagcagat cacccgactg
    19201 gtgccacgcg cgctctggca acgcatcagc ggatcgtgac tttccggcgc atctggtgtg
    19261 tcagacatca aagccgcaaa gtcattagtg aaaacgtaag tcccggtgta atcggggttt
    19321 ttatcgcctg tcacccgcac attacctgcg cagaggaagc aatctggatc atgagcaggt
    19381 aacacctgtt tggctggcgt ttcctgcgcc ccctgccagg ggcgcttagc gcggtgcggt
    19441 gaaaccagaa tccattgccc ggtgagcggg ttgtagcggc gatgtggatg atcaacggga
    19501 ttaaattgcg tcatggtcgt tccttaatcg ggatatccct gtggatggcg tgactgccag
    19561 tgccaggtgt cctgcgccat ttcatcgagt gtgcgcgtta cgcgccagtt cagttcacgg
    19621 tcggctttgc tggcgtccgc ccagtaggcc ggaaggtcgc cctcgcgacg cggtgcaaaa
    19681 tgataattaa ccggtttgcc gcaggctttg ctgaaggcat taaccacgtc cagcacgctg
    19741 ctgcctacgc cagcgccgag gttatagatg tgtacgcctg gcttgttcgc cagtttttcc
    19801 atcgccacga cgtgaccgtc cgccagatcc attacgtgga tgtaatcgcg tacgccagta
    19861 ccgtcttcgg tcggataatc gttaccaaaa atcgccagcg agtcgcgacg gcctacagca
    19921 acctgggcga tgtatggcat caggttattc ggaatgcctt gcggatcttc gcccatatcg
    19981 cccgacggat gcgcgccaac cgggttgaag tagcgcagca gggcaatgct ccagtccggc
    20041 tgggcttttt gcagatcggt gaggatctgt tccaccatca gtttgctttt gccgtaaggg
    20101 ctttgcggtg tgccggtcgg gaagctttca acgtatggaa ttttgggctg atcgccatag
    20161 acggtggcgg aggagctaaa aataaagttt ttgacgttcg cggcgcgcat ggcgctaatc
    20221 aggcgcaggg taccgttgac attgttgtcg taatattcaa gcggtttttg taccgattcg
    20281 ccaacggctt tcagcccggc gaagtggatc acagtgtcga tagcgtgatc gtgcaggatc
    20341 tcggtcatca acgcttcgtt acggatatcg ccttcaacaa acgttggatg tttgccgcct
    20401 aaacgctcga taacaggcag tacgctgcgc ttactgttac agaggttatc aagaatgatg
    20461 acatcatgac cgttttgcag taattgcaca caggtatgac ttccaatgta accgctacca
    20521 ccggtaacca gaactctcat aattcgctcc attaggctta tggtatgaaa taaccatagc
    20581 ataacaaaga tgcgaaaagt gtgacatgga ataaattagt ggaatcgttt acacaagaat
    20641 ttagccgttt tttatgcgcc attaatggat tataaaacag agggtttatg aatgattgag
    20701 ctttttatct gaaaaaagtc gcggtttcat gcctggatgc gtcgaactgt aggccggaga
    20761 gggcgcttag gccgcctccg gtacagtcag cattaccgac tcagtttatt tttgtcagcg
    20821 catagcgata aaagtcaccg tccggcacga actcaagacg atgggtaata caggcaggcg
    20881 catcttcagc atggtgcgaa acaaacaaca attgcgtttc accttcgcta atcagcacat
    20941 caacaaaccg gcggataagc tggcgattga gcggatcaag cccctgtagt ggttcatcga
    21001 gaataagcaa cgtcggatgt ttcaccagcg cgcggacgat cagcgccaga cgctgctgtc
    21061 cccaggaaag actatggaac ggagcgtcag ccgtgcgttt atcaatgccg agaatatcca
    21121 gccactgctg caccagtttt tgctggcgat ccgaaacggc ctgataaatg ccaatcgaat
    21181 caaaatagcc agaaaggatc acattacgca cggtagtgct gacccggtaa tccagatgca
    21241 aactactgct gacgtaaccg atatgctttt tgatatccca gatggtttcg ccgctgccgc
    21301 gacgtcgtcc gaaaagcgtc aaatcgttgc tgtaaccttg cggatgatcg ccagtaatca
    21361 ggcttaataa cgtcgatttt cccgcgccat ttggcccgac aatttgccag tgctcgcctg
    21421 gattcacctg ccagctaagg ttattaagaa tggggcgatc gttataagaa accacgccgt
    21481 tgttcagcac aatgcgcggt tcgttggcgg gtaaggcgtg acgtgctgaa ggttcatccg
    21541 gctccggcag ttgcacacct tcaagctgtt cgctatgtgc cagttgcgcg acgagtgctt
    21601 gctggagcag ttcctcttta gcgcccgttt ccgctaacgt acaatccgcc agcacgccag
    21661 caaactggac aaattccggg atctcatcga agcgattgag caccagtacc agagtaatac
    21721 cggactgatg taacgaggcg agtagctcag ccagctgctg acgtgaggca acatccaggc
    21781 catcgaacgg ctcatcaaga atcaacaagt caggctccga catcagcgcc tgacacagca
    21841 gggtttttcg cgtctcgcca gtggaaaggt atttaaagcg tcggtcgagg agggcggtaa
    21901 taccgaactg ctgcgccagt tgcatgcaac gcggtgcatc ctttacttca tcctgaatga
    21961 tttcagccgt ggtacgcccg gtgtcatctt cgccagggcc gagcatatcg gtgttattcc
    22021 gctgccattc gtcgctgacg agcttttgca attgctcgaa ggagagacga gtgatgtggg
    22081 aaaactggct ttgccgttca cctttcaaaa gcggaagttc ccccgcctgc gcgcgggcca
    22141 gcgccgattt cccgcttcca ttcgaaccga caaacgccca actatcaccc gcgtttaacg
    22201 ttagctgagg caattgcagc gtttttgtgt cgctaagacg aaacgtgcct tgcaaaattt
    22261 gcaacgatga cattttatat cccattttgt gcagcgatta ctgacaggga tacgtgtttc
    22321 ataacaaatt gtcaacacgc ttagcacagc gtggcgataa tcacgctgtc ggcattaaag
    22381 taggccgtga cattctgtcc ttgctgaaga gaagtcgctt catttaccgg cactgtggcg
    22441 cacagtgttt gcccgtcggg tagcgccatt aatacttcgc actgctctgc gccgcgctca
    22501 atatggctaa taatacccgg taattggttg tcggcgtttt gtgcgaccgc ttcgtcctga
    22561 gtaatgccta cccacggcgc ttttagtaat atcaacactt ctttgccttc atccagcccc
    22621 agacgcgcgc cgctttgtgc ggtaattgcg actttcaggc gtgtttttcc gtcagccagt
    22681 aagacatcaa catgctgttg aacgtcatca tgattgcggg cggtgatggt gccaaaccat
    22741 tggttacggg cgctggtttg cagtgaaaaa cgtgagatcg cggccagcag gctgttcagc
    22801 ggcagggcgt catcgtcact taacacatcg aaggcttttt gctggatttg cgccagtaag
    22861 tcatagagct gaatcagtcg ctgaccatag cgggtcagta ctgcgccgcc tccaccttta
    22921 ccgcctgttg cgcgctcaac cagaatatgc tcacttaact gattcatctc gttaatggca
    22981 tcccatgcgc ttttatagct gataccggca tctttcgctc cctggctaat ggaaccggaa
    23041 agcgcaatgt gttttagtag cgaaatgcgg cgcgggtcgg cgaataattt ttgttggagc
    23101 ttaagggtga gaaggatttc ggcctgcata acaatatcct ggaaaaagtc ttattgtgac
    23161 ggaaaacgaa cgccacgcaa agctgaccgc acaaaagggg agtgcttttc tttgcttagc
    23221 ggttagaata gtctcatgac tatatctgga gttgaccatg ttagagttat taaaaagtct
    23281 ggtattcgcc gtaatcatgg tacctgtcgt gatggccatc atcctgggtc tgatttacgg
    23341 tcttggtgaa gtattcaaca tcttttctgg tgttggtaaa aaagaccagc ccggacaaaa
    23401 tcattgaatc cctgaatgcc cgcttagtcg ggcatttctt tttctcaacc tcctgctttt
    23461 cctgccgata ttttttctca tctacctcac aaaggttagc aataactgct gggaaaattc
    23521 tgagttagcc gttatattgt cgcctacata acgttagatt aaggggttac caatggctcg
    23581 taaatggttg aacttgtttg ccggggcggc actctctttc gctgttgctg gcaatgcact
    23641 ggcagatgaa gggaaaatca cggtgttcgc cgccgcatca ctgactaacg caatgcagga
    23701 cattgctacg cagtataaaa aagagaaagg cgtggatgtg gtttcttctt tcgcttcgtc
    23761 atctactttg gcccgtcaga ttgaagcggg tgcgcctgcg gatctgttta tttctgccga
    23821 tcagaaatgg atggattatg cggttgataa aaaagcgatc gatacggctt cgcgccagac
    23881 actgctcggc aatagcctgg tcgttgtagc accgaaagcc agcgagcaga aagatttcac
    23941 catcgacagc aaaaccaact ggacttcgct gctgaatggc ggtcgtctgg cggttggcga
    24001 tccggaacat gttcccgctg gcatttatgc aaaagaagca ctgcaaaaac tgggcgcatg
    24061 ggatacgctc tccccgaaac tggctccggc ggaagatgtt cgtggcgcgc tggcgttggt
    24121 cgaacgcaat gaagcgccgc tgggtattgt ttacggttct gacgcagttg ccagcaaagg
    24181 ggtaaaagtg gttgccacct tcccggaaga ttcgcataaa aaagtggaat atccggttgc
    24241 tgttgtggaa gggcataaca atgcgacggt gaaagcattt tatgattatc tgaagggacc
    24301 gcaggctgcg gaaatcttta aacgttacgg atttacaacc aagtaatgat actgaccgat
    24361 ccagaatggc aggcggtttt actaagcctg aaagtttctt ccctggctgt gctgtttagc
    24421 ctgccgtttg ggatcttttt tgcctggtta ctggtgcgtt gcacgtttcc gggcaaagct
    24481 ctgctcgaca gcgtactgca tctaccgctg gtgttaccgc ccgtggtcgt cggttactta
    24541 ttattagttt cgatgggacg gcgcggattt atcggtgaac gtctgtatga ctggtttggc
    24601 attaccttcg cctttagctg gcgcggcgcg gttctcgctg ccgccgtcat gtcgtttccg
    24661 ctgatggtgc gggcaattcg tctggcgctg gaaggggttg atgtcaaact ggaacaggcc
    24721 gcaagaacac tgggtgccgg gcgctggcgc gttttcttta ctatcacgtt accgctgacc
    24781 ttaccgggca ttattgttgg tacggtactg gcttttgctc gttctctcgg tgagtttggt
    24841 gcaaccatca cctttgtgtc gaacattcct ggtgaaacgc gaaccattcc ttctgccatg
    24901 tataccctga tccagacccc cggcggggaa agtggagcgg cgagactgtg cattatttct
    24961 attgcactgg cgatgatctc cctgttgatt tcagaatggc tggccagaat cagccgtgaa
    25021 cgggcggggc gctaatcatg ctggaactga atttttccca gacgttgggc aaccattgcc
    25081 tgaccattaa tgaaacgctg cccgccaatg gcatcactgc tatctttggc gtctccggtg
    25141 ccggaaaaac ttcgctgatt aacgccatca gtggactgac gcgcccgcaa aaagggcgga
    25201 ttgtcctcaa tgggcgggta ctaaatgatg ccgaaaaagg tatctgcctg acgccggaaa
    25261 agcgtcgcgt tggctatgtt tttcaggatg cgcggctgtt cccgcattac aaagtgcgtg
    25321 gcaatctgcg ctacggcatg gcgaaaagta tggtcgatca gttcgataag ctggtggcgc
    25381 ttttaggcat tgaaccgttg cttgaccgtt taccaggcag cctgtccgga ggtgaaaaac
    25441 agcgcgtggc gattggtcgg gctttgctga cagcaccgga attgctgttg ctggatgaac
    25501 cgctggcgtc actggatatt ccgcgtaaac gcgaactgtt gccttatctg caacggctga
    25561 cacgggaaat caacattccg atgttgtatg tcagccattc gctggatgag atcctccatc
    25621 tggcagacag agtgatggta ctggaaaacg gtcaggtgaa agcctttggc gcgctggagg
    25681 aagtgtgggg cagtagcgtg atgaatccgt ggctgccgaa agagcaacaa agtagcattc
    25741 tgaaagtgac ggtgcttgag catcatccgc attacgcgat gaccgcgctg gcgctgggcg
    25801 atcagcattt gtgggtcaat aagctggacg aaccgctgca agctgcgcta cgcatccgca
    25861 ttcaggcttc cgatgtctcg ctggttttac aaccgccgca gcaaaccagc attcgtaacg
    25921 tattgcgggc aaaagtggtt aatagttatg acgacaacgg ccaggtggaa gtggagctgg
    25981 aagtcggcgg taaaacgctg tgggcgcgta tcagcccgtg ggccagggat gaactggcga
    26041 tcaaacctgg cctgtggctg tatgcgcaaa ttaaaagtgt gtcgataacc gcctgattaa
    26101 atcaggtggc tataaatgaa ctgggcaatg ctgtcggtgg tgttatcacc aatcacaatg
    26161 ttggcgcgcg cttttactgc gtcatcggcg ttgcccatcg ccacgcctgt accagcggct
    26221 tccagcatac taatatcatt gaagttatcg ccgaaggcca cgacgttttc catcgaccag
    26281 ccttgcgcct caacccattt cgtcaaacgt ttacctttgc tgttgccgcc gcgtgcaata
    26341 tcaacctgat cgtgccagga ccattcacac tccagtccca gttcatgttc gacatgctta
    26401 ccaaaatgct gcaattgcgg caggtcatcg tgcgtcaggg cgaacttcca tacggcgtta
    26461 acttgttgcg ccgtttcagc cagagaagcg acttgtgtga aagtcggacg ctgttccggc
    26521 ggcagggttt gcgcccagtt agacgtgcga atgacatgtc cggtcgggtg ctcatagacc
    26581 attgcatcat cgacatacat cagaccgtga atgtggtgtt cattcagcat ctcaatgagt
    26641 tgcagggctt tattaacggg cattgggtcc gcttccagca ctgtttttgc atgataatca
    26701 tacaaatagg tgccattaca gcaaatagca ggtgtatcca gcgccagcgc ctgataaaaa
    26761 ggatgaatag cgacgtgatg gcgacctgtg acgatgatta attgatagcc tgcttcgcga
    26821 gcgcgggcca gggcttctat cgatgatgga agcaaggtct ttttcggggt caataaggtg
    26881 ccgtctaagt cgagagcaat cacgcgtgtg gtcatggcgt attccagatt aaggttaaga
    26941 attttcgtct gcgcgaatgg tacaccgata ccactatcgg acaaaattct gcgttttaat
    27001 tcagcattca ccgccaaaag cgactaattt tagctgttac agtcagttgc taaatgcaaa
    27061 ggagcattca tgaagcaaac agtttatatc gccagccctg agagccagca aattcacgtc
    27121 tggaacctga atcatgaagg cgcactgacg ctgacacagg ttgtcgacgt gccggggcag
    27181 gtgcagccga tggtggtcag cccggacaaa cgttatctct atgttggtgt tcgccctgag
    27241 tttcgcgtcc tggcgtatcg tatcgccccg gacgatggcg cgctgacctt tgccgcagag
    27301 tctgcgctgc cgggtagtcc gacgcatatt tccaccgatc accaggggca gtttgtcttt
    27361 gtcggttctt acaatgcggg taacgtgagc gtaacgcgtc tggaagatgg cctgccagtg
    27421 ggcgtcgtcg atgtggtcga ggggctggac ggttgccatt ccgccaatat ctcaccggac
    27481 aaccgtacgc tgtgggttcc ggcattaaag caggatcgca tttgcctgtt tactgtcagc
    27541 gaggatggtc atctggtggc gcaggaccct gcggaagtga ctaccgttga aggggccggc
    27601 ccgcgtcata tggtattcca tccaaacgaa caatatgcgt attgcgtcaa tgagttaaac
    27661 agctcagtgg atgtctggga actgaaagat ccgcatggca atatcgaatg tgtccagacg
    27721 ctggatatga tgccggaaaa cttctccgac acccgttggg cggctgatat tcatatcacc
    27781 ccggatggtc gtcatttata cgcctgcgac cgtaccgcca gtctgattac tgttttcagc
    27841 gtttcggaag atggcagcgt gttgagtaaa gaaggcttcc agccaactga aacccagccg
    27901 cgcggcttca atgttgatca cagcggcaag tatctgattg ccgccgggca aaaatctcac
    27961 cacatctcgg tatacgaaat tgttggcgag caggggctac tgcatgaaaa aggccgctat
    28021 gcggtcgggc agggaccaat gtgggtggtg gttaacgcac actaaccgct gatttacccg
    28081 gcgcagtctc ttctgcgccg gtgtattaac ctatctcctg taacgcatgt ctctggcgtt
    28141 cgacgatatt ggtccacaaa ttgtctttat cgtcagtcca caaattaatc agcaaggcaa
    28201 aaaagcgttc agctgccgga gaaagcacgg catctttgcg gcgaataatc cccaatgtcc
    28261 gacgtataac cggttccacc agcgggatac caataatcga agaatagggc gcatggggca
    28321 ttgccagacc aggaagcgcc gaaatcccca gtcccgcctc caccagccct aatgacgtcg
    28381 aaagatgacg cacttcgtaa aaccagtcca gcttccaggg cttgtcggct agctgttgtt
    28441 ctatcaatag tcggttgccg ctggaggagc gtacgccaat cattttgtaa cccactaact
    28501 cctgccattc tacaagctgc tttttggcca atggatggtc acgccgacag gccagcacga
    28561 acggctcgtt gaccagtggg gtaaaatcaa tggatgaatt tgtgacgttg ttcatgttta
    28621 tgccaaagtc tgattcgtta cacagcaccg attccatgca attattcgtg ccttgttcca
    28681 gaatccgcac tttaatattg ggatacagct cattaaattt accgattgcc agcggtaaaa
    28741 aataaaatac tgcggtcgga atacacgcta acgtcaccat accacgatga taagcattca
    28801 tatcacgaat attaaaaagc gtttcatcga atttttttat taattcccgc gcctccggga
    28861 gcaacctttt tcctgctttt gttaacgtaa ccttgcgagt tgtacgctca aatagttgaa
    28921 tgtgtaaatc ttcctccatc ttttttattc ttcgcgttaa tgcaggttgc gtaatattga
    28981 gtaatttagc agcattgtta aatgaactgg actctgccag tatgacaaat gccttcatac
    29041 ttgataattc atgcttcatt attactccgg aaaatggaag cgacgatttt gggtgactgg
    29101 cagttaaaaa ttttaactgc atttagccaa cttaaattaa tgaaaaaatg ttattaatcg
    29161 ttgagctaaa gtcattagag atgctttgcc cttaatgtaa ccatatcgca ataagttatg
    29221 tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc gaggtggaac
    29281 ctcgaggggc gcgttcctgt tagcggaaca tctacccgaa gatcaaacgc agcgcgataa
    29341 aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta ttggcggcgg
    29401 taatccgctg accagtaaag tcgccattat tagccgctcc agcgatccgc gtgcagatgt
    29461 cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca cgcctaactg
    29521 cggcaatatg ctgtctggcg ttggggcatt cgccattgaa aatggtttga ttgcagcgac
    29581 ttcgccagtt acccgcgtac gtatccgcaa cgtcaatacg ggtacgttta tcgaagctga
    29641 tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg acggcgtacc
    29701 gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gccggaacga aaaccggaaa
    29761 agtcttcccg acggataatc agattgatta ttttgacgat gtcccggtga cctgtatcga
    29821 tatggcgatg ccagttgtca ttattcctgc tgaatatctg ggaaaaacag gctatgaatt
    29881 accggcagag ctggatgccg acaaagcatt attagccagc attgaatcta tccgtctaca
    29941 agcgggtaaa gcaatgggct taggcgatgt cagtaatatg gttatcccta aacctgtact
    30001 tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc cgcattcttg
    30061 ccatcgcgct ttggcgataa ccggtgctat tgctatttcc tgtagttgtg cattggaagg
    30121 caccgtcacc cgacaaatcg tcccttctgt aggatacggc aatatcaata ttgaacaccc
    30181 cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga cgttacgcgc
    30241 atctgttatt cggacgacaa gaaaaatatt ttccggtgaa gtttatcttc cctgaaaaat
    30301 tttgttgtcg tgataatgac aatcaataaa ggaattctgt atgagtcata cagaaagaac
    30361 aggatattaa atgaataaaa aatcgttatg gaagctaatt ctgatattag cgatcccatg
    30421 tattattggc tttatgccag ctccggcagg attaagcgaa ctggcgtggg tgctttttgg
    30481 tatttacctg gcggccattg tggggctggt tatcaagcct ttcccggaac ctgtcgtact
    30541 gttaattgcc gtcgccgcat cgatggtagt ggtgggtaac ttatcaggtg gggaatttaa
    30601 aaccaccgcc gtattaagcg gttactcttc cggtaccacc tggctggtgt tttctgcgtt
    30661 tactttaagc gccgcgtttg taaccacagg cttaggtaaa cgtattgcct atctgctgat
    30721 tggtaaaatt ggtagcacta ccctgggtct gggttacgtt acggtatttc tcgatctggt
    30781 actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcatcgtgt taccgatcat
    30841 caacagcgtg gcagtggctt tgggatcaga accggaaaaa agtccgcgtc gtgttggaca
    30901 ttacctgatg atgtccattt acatggtcac taaaaccacc agctatatgt tctttaccgc
    30961 aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc tgcaaattag
    31021 ctggggtgga tgggcgctgg ccgctggatt gccgggtatc attatgctgc tggtcacccc
    31081 gctggtgatt tacaccatgt atccgccaga aattaagaag gtggataaca aaaccatcgc
    31141 caaagcgggc cttgccgaac tgggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt
    31201 cttcgtgctg gcgctgctgg gctggatttt cagtaagtca ctgggggttg atgaatccac
    31261 cgtggcaatc gttgttatgg cgactatgct gctgctgggt atcgttacct gggaagacgt
    31321 ggttaaaaat aaaggcggct ggaatacctt aatctggtac ggcggtatta tcggcttaag
    31381 ctccttatta tcgaaagtta aattctttga atggttagct gaagtcttta aaaataacct
    31441 ggcatttgat ggtcacggta acgttgcttt cttcgttatt attttcctca gcatcatcgt
    31501 gcgttatttc ttcgcttccg gtagtgccta tatcgttgct atgttacctg tatttgccat
    31561 gctggcgaac gtctccggcg caccgttaat gttaaccgcg ctggcactgt tgttctctaa
    31621 ctcctatggc ggcatggtta ctcactatgg cggcgcggca ggtccggtca tctttggcgt
    31681 gggttacaac gatattaaat cctggtggtt ggtcggtgcg gtactgacga tattaacctt
    31741 cctggtgcat atcaccctcg gcgtgtggtg gtggaatatg ctgatcggct ggaacatgct
    31801 gtaaatatac tcgtcatact tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt
    31861 cacttactta tgtaagctcc tggggattca ctcgcttgtc gccttcctgc aactcgaatt
    31921 atttagagta tatccattta ttatctttct gcgcacttca cggtgcgcag atatctggag
    31981 catttgatga tcaagttatc tgaaaaaggc gtgtttctcg ccagcaataa cgaaataatt
    32041 gccgaagaac atttcaccgg cgaaataaaa aaagaagaag caaaaaaagg cactattgcc
    32101 tggtctattc tctcttctca taatacgtcc ggaaatatgg ataaacttaa aattaagttt
    32161 gattcattag cctctcacga tattaccttt gttggtattg tacagaccgc taaagcgtcc
    32221 ggtatggaac gtttcccgct gccgtatgtg ctgaccaact gccataactc actctgtgct
    32281 gtgggcggca ccattaacgg tgatgaccat gtttttggtt tatcggcggc ccagcgttat
    32341 ggcggtattt ttgtgccacc gcatattgcg gtcatccatc aatatatgcg tgaaatgatg
    32401 gcgggcggcg gcaaaatgat cctcgggtca gacagccata cccgttacgg tgcgttaggg
    32461 acaatggcgg ttggcgaggg cggcggcgag ttggtaaaac agctgcttaa tgacacctgg
    32521 gatatcgact atccgggcgt ggttgcggtg catctgaccg gaaaacctgc gccgtatgtg
    32581 gggccgcagg atgtggcgct ggctatcatc ggtgccgtgt tcaaaaacgg ttacgtcaaa
    32641 aacaaagtga tggagttcgt aggtcccggt gttgcagcgc tctctaccga tttccgtaac
    32701 agcgttgacg tgatgaccac tgaaacgacc tgtttaagtt ctgtctggca aaccgatgaa
    32761 gaagtccata actggctggc gctgcacggt cgcggccagg attactgcca acttaaccct
    32821 caaccaatgg cgtactacga tggctgcatc agcgttgatt taagcgccat caaaccaatg
    32881 attgcgctgc cattccaccc gagcaacgtg tatgaaatcg acacgctgaa ccagaacctg
    32941 accgacattc tgcgtgagat tgaaattgag tccgaacgcg tggcgcacgg taaagccaaa
    33001 ctctcgctgc tggataaagt ggaaaatggt cgcctgaaag tgcagcaggg gattatcgcg
    33061 ggctgttctg gcggtaacta cgaaaacgtc atcgcggcgg cgaatgcgct gcgcggtcaa
    33121 tcctgcggca acgatacctt ctcattggcg gtttacccgt catcacagcc ggtgtttatg
    33181 gatctcgcca aaaaaggtgt ggtagcagat ttgattggcg caggcgcaat catcagaacc
    33241 gcgttctgcg gcccatgctt tggtgcgggc gatacgccaa tcaacaacgg tttgagtatt
    33301 cgccacacca cgcgtaactt cccgaaccgc gaaggctcta agccagctaa tgggcagatg
    33361 tcagcggtgg cgttgatgga cgctcgttct atcgctgcaa ctgcggcaaa cggtggctat
    33421 ttaacctctg ccagcgaact cgattgctgg gacaacgtgc cggagtacgc cttcgatgta
    33481 acgccgtata aaaatcgcgt ctatcaaggc tttgtgaaag gggcgaccca gcaaccgctg
    33541 atctacggac cgaacatcaa agactggccg gaattgggcg cgctgactga caatatcgtc
    33601 ctcaaagtgt gctcgaagat cctcgacgaa gtcaccacca ccgacgaact gattccttcc
    33661 ggtgaaacct cttcttatcg ttcaaatccg attggtctgg cggagtttac cctgtcacgc
    33721 cgcgatcccg gttatgttgg cagaagtaaa gcgactgctg agctggaaaa tcagcgtctg
    33781 gcggggaatg tcagcgagct gacagaggtg tttgcgcgca ttaagcagat tgctggtcag
    33841 gagcatattg atccgctgca aactgaaatt ggcagcatgg tctatgcggt gaaaccaggc
    33901 gatggttctg cgcgtgagca ggcggcgagc tgccagcgtg tgattggcgg tctggcgaat
    33961 attgccgagg aatacgcgac taaacgctac cgttctaacg tcatcaactg ggggatgtta
    34021 ccgctgcaaa tggcggaagc accaacgttt gaagtggggg attacattta catccctggc
    34081 attaaagcgg cgctggataa tcctggaacg acgtttaaag gttatgtgat ccatgaagat
    34141 gcaccggtaa cggagattac gctctatatg gaaagtctga ctgctgaaga gcgcgagatt
    34201 atcaaggcgg gtagtttgat taacttcaat aaaaaccgtc agatgtaaaa agcgccatgt
    34261 gaatgtaggt cgcatccggc acttattgtc ggatgcgatg ctggcgcatc ttatccgacc
    34321 tacaaatcgc atcgaaccgt aggccggata aggcgtttac gccgcatccg gcacatagtt
    34381 aacagctcct acttcttcgc ctctgcaacc actttgctac ccacgccgcg gttattgtat
    34441 tcccacatgc ggttgtagtt agtgtcattc agattgcgct gtacttcgtc gttatcatca
    34501 acgctgccgg tgttacccgc gaatggacga ttggaaatca ccgcatcagc ccacggtttg
    34561 gctgtgttaa aaccttcgtt gatggcgcta tcacggatca ccacctgacc gttggtattg
    34621 gcatcaacat ccagcgagcg gcccagttgc gccacgccat caccggaagc attgaaacga
    34681 ctgtttacgg cgaggaaacc gtagtaaatg ttggacagcg tagccggtgc aaacacatac
    34741 gcttcttgct gagtacgtga gttcaccacg cggaattcgg tattatcgaa caccactgcg
    34801 ccgcgaccag aaacgatatc cacatcccct tcaatgtagc tgttggtcac cagcgtacgc
    34861 ggctgacgat tcgtttccag acggttctgc acaccgctgt tggtgacaaa gaaggtgttc
    34921 tgacgaccga gaatgttaac gttgttaatc tgtacctggt caccatcagt acgcagtgcc
    34981 accgccggat ggttacccgc atctacgcta tcgcccagcg tgttttcgat ggtcaggttt
    35041 tgcagttgca gtccattgtt ttgtgaccag aagaccgcag agcagagaac gccgatactg
    35101 tcgctgcgtt tgctctggca gctatcgtac atataccacg ctggtttacc tggcatatat
    35161 ttgccgcgcg ggttgacgtc gtgacgccag tcggcggggc tcatgccccc atcaagggaa
    35221 agcccaatct tcacatcaat cggtttttca cctgtaccgt acagagtaat tccacccgga
    35281 gcggcaggga catataccgt tccctgatac tcaccaggca tcacggcaat atactggcgc
    35341 ttgttggtac gcttgataat tgccgcatct accgccgcct gaatcgtggt atgcgttaca
    35401 ccttgagtac ccgccgggcc gacaacaaag tcaggttgtg caggcagggt aatcggggaa
    35461 ggattccacg ctgccgcacc tggtgtcagg gatgcaaaat agtgttgagc atcgaaattc
    35521 tgcgcttctt ttgccgacag aatcgggcga gaagaggtac caggcgcggt ttgatcagaa
    35581 ggacgttgat cgggcggtgt tgagctacag gcggtcagcg tcacgccaaa agccaatgcc
    35641 agcgccagac gggaaactga aaatgtgttc acaggttgct ccgggctatg aaatagaaaa
    35701 atgaatccgt tgaggcctgc ttttttatac taactttagc gaaacgggaa ggtaaaaaga
    35761 caaaaagttg tttttaatac ctttaagtga taccagatgg ctttgcgcca tctggcagag
    35821 taattaactg aacatcgcgg taatcgatgc gcttgccaga gagtggaaat ggatgttaaa
    35881 cccgaccatc gcgccgctgg caccttcatc gacatcaata cgttctacat ccagcgcatg
    35941 aacggtaaaa atgtagcgat gggtttcgcc tttcggcggc gctgcaccat cgtacccggt
    36001 tttaccaaag tcggtacgcg tctgcaaaac gccgtctggc attgctacca gaccagagcc
    36061 aaacccttgc ggtaatacgc gggtatcagc gggtaagtta acaactaccc agtgccacca
    36121 gccggagccg gttggcgcat ccgggtcgta gcaggtgaca acaaaacttt tcgttcccgc
    36181 aggaacatca tcccacgcca gatgcggtga aatattatcg ccatcgtaac ccatgccgtt
    36241 aaagacatga cgatgtggca acttatcgcc atcgcgcaga tcattactga tgagtttcat
    36301 gaaccctcct ttctagtttg cagaaagtgt agccagaacc cctcacgctg actcccggtt
    36361 attggcaaaa aaatgtttca tcctgcaccg cacggttaac cgctacggtc agacgctgca
    36421 actgttgcgg gagaataata tagggcggca tcaggtaaat cagtttgcca aaaggccgga
    36481 tccagacacc ctgttcgaca aagaattttt gcagcgctgc catattcacc ggacgagtgg
    36541 tttcgaccac gccgattgcc cccagtacgc gcacatcggc aaccatttcg gcgtcacagg
    36601 cgggggcaag ttgctcgcgt agctgccctt caatagccgc cacctgatgc tgccagtcgc
    36661 cagattcgag aatcgccagg ctggcgtttg ctgctgcgca ggccagcgga ttgcccataa
    36721 aagttggccc atgcataaag cagccggctt cgccgttact gatggtttcc gcaacctcgc
    36781 gcgtggtgag cgtggcggaa agggtcattg tgccgccggt taaggcttta ccgagacaca
    36841 aaatatccgg cgcgatatct gcatgttcac aggcaaacag tttgccggta cgaccaaatc
    36901 cggtggcgat ctcgtcagca atcagcaaga taccttcgcg atcgcacatt ttgcggattc
    36961 gttttaacca ttccggatgg tagatgcgca tcccgcctgc gccctggaca atcggttcaa
    37021 tgatcacggc ggcgatttca tgacgatgtg ccgccatcag gcgggcaaag cccaccatat
    37081 cgcgttcatc ccattcacca tccatgcggc tttgcggggc gggagcaaac aagttttctg
    37141 gcagatagcc tttccacagg ctgtgcattg agttgtccgg atcgcacaca gacatcgcgc
    37201 caaaggtatc gccatgataa ccattgcgga aggtcagaaa acgctggcgc gcttcgcctt
    37261 tggcctgcca gtactgcaac gccattttca tcgccacttc caccgctacg gaaccggagt
    37321 ccgcgagaaa aacgcattcc agcggttgcg gcgtcatcgc caccagtttg cggcacagct
    37381 caatggctgg cgcatgagtg ataccaccaa acatcacatg cgacatggca tcaatttgcg
    37441 tcttcatcgc cgcattaagc tgcgggtgat tgtagccgtg gatcgccgcc caccaggacg
    37501 acataccgtc aaccaggcgt ctgccgtcag acaaaattag ctcgcaacct tcggcgctcg
    37561 ccaccggata aaccggtagc ggggaggtca tggatgtgta tgggtgccag atatggcgtt
    37621 ggtcaaaggc aagatcgtcc gttgtcataa tcgacttgta aaccaaattg aaaagattta
    37681 ggtttacgag tctacaccga attaacaaca aaaaacacgt tttggagaag ccccatggct
    37741 caccgcccac gctggacatt gtcgcaagtc acagaattat ttgaaaaacc gttgctggat
    37801 ctgctgtttg aagcgcagca ggtgcatcgt cagcatttcg atccacgtca ggtgcaggtc
    37861 agcactttgc tgtcaattaa gaccggagct tgtccggaag attgcaaata ctgcccgcaa
    37921 agctcgcgct acaaaaccgg gctggaagcc gagcggttga tggaagttga acaggtgctg
    37981 gcgtcggcgc gcaaagcgaa agcggcagga tcgacgcgct tctgcatggg cgcggcgtgg
    38041 aagaatcccc acgaacgcga tatgccctac ctggaacaaa tggtgcaggg agtaaaagcg
    38101 atggggctgg aggcgtgtat gacgctgggt acgttgagtg aatctcaggc gcagcgcctt
    38161 gcgaatgccg ggctggatta ctacaaccat aacctcgaca cctcgccgga gttttacggc
    38221 aatatcatca ccacacgtac ttatcaggaa cgccttgata cgctggaaaa agtgcgcgat
    38281 gccgggatca aagtctgctc aggtggcatt gtgggtttag gcgaaacggt aaaagatcgt
    38341 gccggattat tactgcaact ggcaaacctg ccgacgccgc cggaaagcgt gccaatcaac
    38401 atgctcgtga aggtgaaagg tactccactg gcagataacg atgatgtcga tgcctttgat
    38461 tttattcgca ccattgccgt agcgcggatc atgatgccga cctcatacgt gcgcctttct
    38521 gccggacgcg agcagatgaa tgaacagact caggcgatgt gctttatggc cggggctaac
    38581 tcgattttct acggttgcaa actgctaacc acgccgaatc cggaagaaga taaagacctg
    38641 caactgttcc gcaaactggg gctaaatcca cagcaaactg ccgtgctggc aggggataac
    38701 gaacaacagc agcgtctgga gcaggcactg atgaccccgg acactgacga atattacaac
    38761 gcggcagcac tatgagctgg caggagaaaa tcaacgcggc gctcgatgcg cggcgtgctg
    38821 ccgatgccct gcgtcgccgt tatccggtgg cgcaaggggc cggacgctgg ctggtggcgg
    38881 acgatcgcca gtatctgaac ttttccagta acgattattt aggtttaagc catcatccgc
    38941 aaattatccg tgcctggaag cagagtgcgg agcaatttgg cgtcggtagc ggcggctccg
    39001 gtcacgtcag cggttatagc gtggcacatc aggcgctgga agaagaactg gccgagtggc
    39061 tgggctattc gcgggcgctg ctgtttatct ccggttttgc ggctaatcag gcagtcattg
    39121 ccgcgatgat ggcgaaagag gaccgtattg ttgccgaccg gcttagccat gcctcattgc
    39181 tggaggctgc aagtttaagc ccgtcgccgc ttcgccgttt tgctcataac gatgtcactc
    39241 atctggcgcg actgcttgct tccccctgtc cggggcagca actggtagtg acagaaggtg
    39301 tgttcagcat ggacggcgat agtgcgccac tggaggaaat ccagcaggta acgcaacagc
    39361 acgatggctg gttgatggtc gatgacgccc acggcacggg cgttatcggg gagcaggggc
    39421 gcggcagctg ctggctgcaa aaggtaaaac cagaattgct ggtggtgact tttggcaaag
    39481 gatttggcgt cagcggggca gcggtgcttt gctccgatac ggtggcggat tatctggtgc
    39541 aatttgcccg ccatcttatt tacagcacca gtatgccgcc cgctcaggcg caggcattac
    39601 gtgcatcgct ggcggtcatt cgcagtgatg agggtgatac acggcgcgaa aaactggcgg
    39661 cactcattac gcgttttcgt gccggagtac aggatttgcc gtttacgctt gctgattcat
    39721 gcagcgccat ccagccattg attgttggtg ataacagccg tgcgttacaa ctggcagaaa
    39781 aactgcgcca gcaaggctgc tgggtcacgg cgattcgccc gccaaccgta cctgctggta
    39841 ctgcgcgact gcgcttaaca ctaacagccg cgcatgaaat gcaggatatc gaccgtctgc
    39901 tggaggtgct gcatggcaac ggttaataaa caagccattg cagcggcatt tggacgggca
    39961 gccgcacact atgagcaaca cgccgatcta cagcgccaga gtgctgacgc cttactggca
    40021 atgctcccac agcgtaaata cacccacgta ctggatgcgg gttgtggacc aggctggatg
    40081 agccgccgct ggcgagagcg tcacgcgcag gtgactgcgt tagatctctc tccgccaatg
    40141 cttgttcagg cacgccagaa ggatgccgca gaccattatc tggcgggaga tatcgaatcc
    40201 ctgccgttag cgactgcaac gttcgatctt gcatggagca atctcgcggt gcagtggtgc
    40261 ggtaatttat ccatggcact ccgcgagctg tatcgggtgg tgcgccccgg aggcgtggtc
    40321 gcgtttacca cgctggtgca gggatcgtta cccgaactgc atcaggcgtg gcaggcggtg
    40381 gacgagcgcc cgcatgctaa tcgcttttta ccgccagata aaatcgaaca gtcgctaaac
    40441 gccgtgcatt atcaacatta tattcagccc attacgctgt ggtttgatga tgcgctcagt
    40501 gccatgcgtt cgctgaaagg catcggtgcc acgcatcttc atgaagggcg cgacccgcga
    40561 atattaacgc gttcgcagtt gcagcgattg caactagcct ggccgcaaca acaggggcga
    40621 tatcctctga cgtatcatct ttttttggga gtgattgctc gtgagtaaat gttattttgt
    40681 caccggaacg gataccgaag tggggaaaac tgtcgccagc tgcgcgcttt tacaagccgc
    40741 aaaggcagca ggctaccgga cggcaggtta taaaccggtg gcctctggca gcgaaaagac
    40801 cccgaaaggc ttacgtaata gtgacgcgct ggcgttacag cgcaacagta gcctgcagct
    40861 ggattacgca acggtaaatc cttacacctt cgcagaaccc acctcgccgc atatcatcag
    40921 cgcgcaagag ggcaggccga tagaatcatt ggtaatgagc gccggattac gcgcgcttga
    40981 acaacaggct gactgggtgt tagtggaagg tgctggcggc tggtttacgc cgctttctga
    41041 cactttcact tttgcggatt gggtaacaca ggaacaactg ccggtgatac tggtagttgg
    41101 tgtgaaactc ggctgtatta atcacgcgat gttgactgcg caggcaatac tacacgccgg
    41161 actgactctg gcggggtggg tggcgaacga tgtcacgcct ccgggaaaac gtcacgctga
    41221 atatatgacc acgctcaccc gcatgattcc cgcgccgctg ctgggcgaaa ttccctggct
    41281 tgcagaaaat ccagaaaatg cggcaaccgg aaagtacatc aatcttgcct taatgtagtc
    41341 atcctgtaat tg
//
LOCUS       DAZKJR010000026        41078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKJR010000026 DAZKJR010000000
VERSION     DAZKJR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.026"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(183..339)
                     /gene="hokD"
                     /locus_tag="ACHOWS_001430"
                     /pseudo
     CDS             complement(183..339)
                     /gene="hokD"
                     /locus_tag="ACHOWS_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(539..964)
                     /locus_tag="ACHOWS_001431"
     CDS             complement(539..964)
                     /locus_tag="ACHOWS_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFQ0047034.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIELEMDRYVERERSGAQVSQFSGHTGN"
     gene            complement(1043..1906)
                     /locus_tag="ACHOWS_001432"
     CDS             complement(1043..1906)
                     /locus_tag="ACHOWS_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0047035.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGID
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1906..2874)
                     /locus_tag="ACHOWS_001433"
     CDS             complement(1906..2874)
                     /locus_tag="ACHOWS_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0047036.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSGLTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2876..4534)
                     /locus_tag="ACHOWS_001434"
     CDS             complement(2876..4534)
                     /locus_tag="ACHOWS_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0047037.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLGQ"
     gene            complement(4568..4936)
                     /locus_tag="ACHOWS_001435"
     CDS             complement(4568..4936)
                     /locus_tag="ACHOWS_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047038.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRKGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
     gene            complement(5180..5473)
                     /locus_tag="ACHOWS_001436"
     CDS             complement(5180..5473)
                     /locus_tag="ACHOWS_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047039.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
     gene            complement(5492..5701)
                     /locus_tag="ACHOWS_001437"
     CDS             complement(5492..5701)
                     /locus_tag="ACHOWS_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047040.1"
                     /translation="MTLYEILKAEFKTNAAIGRRFPKRGKPRGSQGVGKWKTRGVPED
                     VAILCHLDPKIPYQHPSLMNASHES"
     gene            5812..6468
                     /locus_tag="ACHOWS_001438"
     CDS             5812..6468
                     /locus_tag="ACHOWS_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFQ0047041.1"
                     /translation="MRTLGERLSSARQRAGLTQDALAKKAGITRVAISKAEQGLTKSF
                     NGDTLFKVATALNCTPQWLQNGEEQSVNWENNVKSCPQKDAFHSYPVINWVQAGLFST
                     AGDDYSMYDHDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKAVHS
                     GCYVIARKKSTNEATFKKYVTEMGRSFLKPLNPQYPIIEIDDDCEIVGVIVDARWDIF
                     "
     gene            6553..6963
                     /locus_tag="ACHOWS_001439"
     CDS             6553..6963
                     /locus_tag="ACHOWS_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0047042.1"
                     /translation="MFQKTTLSCIEDLKNLGSLIALIAKAAPDTTLANDIESCAGMAW
                     DIINKVSHTLSLLIHKQNISDCPHEIRTQRESCGLSTAELATLLGVDEETIIQWENGK
                     AEPLASQVIPLANILDCDPMWLLTGFHSDTTKES"
     gene            6965..7156
                     /locus_tag="ACHOWS_001440"
     CDS             6965..7156
                     /locus_tag="ACHOWS_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047043.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            7153..7353
                     /locus_tag="ACHOWS_001441"
     CDS             7153..7353
                     /locus_tag="ACHOWS_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047044.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKRN"
     gene            7528..7890
                     /locus_tag="ACHOWS_001442"
     CDS             7528..7890
                     /locus_tag="ACHOWS_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047045.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            7931..8125
                     /locus_tag="ACHOWS_001443"
     CDS             7931..8125
                     /locus_tag="ACHOWS_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047046.1"
                     /translation="MNNCVEVLDNVHCEITESLSLLQVIIDSGDIGSPDALNMMNCLF
                     RSLSCTADKAEKHLCRTENE"
     gene            8298..8858
                     /locus_tag="ACHOWS_001444"
     CDS             8298..8858
                     /locus_tag="ACHOWS_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047047.1"
                     /translation="MKEEPEIIEINSEEFAENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            8845..9429
                     /locus_tag="ACHOWS_001445"
     CDS             8845..9429
                     /locus_tag="ACHOWS_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047048.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            9453..10322
                     /locus_tag="ACHOWS_001446"
     CDS             9453..10322
                     /locus_tag="ACHOWS_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFQ0047049.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGTTPIRIQQ
                     NHHENKSRFIEGKHFFKVVGDELKNLRLVLNESQNEMKISPKTRSLILWTERGAANHA
                     KLLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMVLENKTL
                     SATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTA
                     SYARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFK
                     HDKAIYTPEAEHE"
     gene            10315..10677
                     /locus_tag="ACHOWS_001447"
     CDS             10315..10677
                     /locus_tag="ACHOWS_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0047050.1"
                     /translation="MNNTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQNEYTGETNIMATIQVGDLACTDEGMLGEAWLA
                     DMLIAAEIIRIEPAGTLS"
     gene            10694..10918
                     /locus_tag="ACHOWS_001448"
     CDS             10694..10918
                     /locus_tag="ACHOWS_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047051.1"
                     /translation="MIISFTDYGEQAKLVITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            10915..11817
                     /locus_tag="ACHOWS_001449"
     CDS             10915..11817
                     /locus_tag="ACHOWS_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047052.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            11810..12052
                     /locus_tag="ACHOWS_001450"
     CDS             11810..12052
                     /locus_tag="ACHOWS_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047053.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKARAPAQPNELA"
     gene            12073..12219
                     /locus_tag="ACHOWS_001451"
     CDS             12073..12219
                     /locus_tag="ACHOWS_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047054.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            12227..12481
                     /gene="xisR"
                     /locus_tag="ACHOWS_001452"
     CDS             12227..12481
                     /gene="xisR"
                     /locus_tag="ACHOWS_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFQ0047055.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            12515..13804
                     /locus_tag="ACHOWS_001453"
     CDS             12515..13804
                     /locus_tag="ACHOWS_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0047056.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            13849..14499
                     /gene="rcsB"
                     /locus_tag="ACHOWS_001454"
     CDS             13849..14499
                     /gene="rcsB"
                     /locus_tag="ACHOWS_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFQ0047057.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(14585..17434)
                     /gene="rcsC"
                     /locus_tag="ACHOWS_001455"
     CDS             complement(14585..17434)
                     /gene="rcsC"
                     /locus_tag="ACHOWS_001455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFQ0047058.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            17601..19426
                     /gene="atoS"
                     /locus_tag="ACHOWS_001456"
                     /pseudo
     CDS             17601..19426
                     /gene="atoS"
                     /locus_tag="ACHOWS_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
     gene            19423..20808
                     /gene="atoC"
                     /locus_tag="ACHOWS_001457"
     CDS             19423..20808
                     /gene="atoC"
                     /locus_tag="ACHOWS_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFQ0047059.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            21004..21666
                     /gene="atoD"
                     /locus_tag="ACHOWS_001458"
     CDS             21004..21666
                     /gene="atoD"
                     /locus_tag="ACHOWS_001458"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFQ0047060.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            21666..22316
                     /gene="atoA"
                     /locus_tag="ACHOWS_001459"
     CDS             21666..22316
                     /gene="atoA"
                     /locus_tag="ACHOWS_001459"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFQ0047061.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            22313..23635
                     /gene="atoE"
                     /locus_tag="ACHOWS_001460"
     CDS             22313..23635
                     /gene="atoE"
                     /locus_tag="ACHOWS_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFQ0047062.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPIMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            23666..24850
                     /gene="atoB"
                     /locus_tag="ACHOWS_001461"
     CDS             23666..24850
                     /gene="atoB"
                     /locus_tag="ACHOWS_001461"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFQ0047063.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATKKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(24924..25700)
                     /gene="yfaP"
                     /locus_tag="ACHOWS_001462"
     CDS             complement(24924..25700)
                     /gene="yfaP"
                     /locus_tag="ACHOWS_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFQ0047064.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(25705..27354)
                     /gene="yfaQ"
                     /locus_tag="ACHOWS_001463"
     CDS             complement(25705..27354)
                     /gene="yfaQ"
                     /locus_tag="ACHOWS_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFQ0047065.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVNSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKITAEKSS
                     TAVKPGVLNGRYRVTFANGNHIMFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTFQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(27355..31869)
                     /locus_tag="ACHOWS_001464"
     CDS             complement(27355..31869)
                     /locus_tag="ACHOWS_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFQ0047066.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHCIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARIPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTEKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIQLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPILHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGKLAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(31893..32516)
                     /gene="yfaT"
                     /locus_tag="ACHOWS_001465"
     CDS             complement(31893..32516)
                     /gene="yfaT"
                     /locus_tag="ACHOWS_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFQ0047067.1"
                     /translation="MRHGLLALICWLCCVVTHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(32513..34201)
                     /gene="yfaA"
                     /locus_tag="ACHOWS_001466"
     CDS             complement(32513..34201)
                     /gene="yfaA"
                     /locus_tag="ACHOWS_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFQ0047068.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKAQAVQPDQLMSDYFFRVSLA
                     MQNKTMLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(34350..36977)
                     /gene="gyrA"
                     /locus_tag="ACHOWS_001467"
     CDS             complement(34350..36977)
                     /gene="gyrA"
                     /locus_tag="ACHOWS_001467"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFQ0047069.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLIIQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            37124..37846
                     /gene="ubiG"
                     /locus_tag="ACHOWS_001468"
     CDS             37124..37846
                     /gene="ubiG"
                     /locus_tag="ACHOWS_001468"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFQ0047070.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(37986..>41078)
                     /gene="yfaL"
                     /locus_tag="ACHOWS_001469"
     CDS             complement(37986..>41078)
                     /gene="yfaL"
                     /locus_tag="ACHOWS_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFQ0047071.1"
                     /translation="KTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNN
                     GVMIDENNSASGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAG
                     TGVIVKEGAGELVLNADNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGL
                     MVGSTVHSEYQAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEG
                     QLTVAQDGSYLLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVL
                     DLSDFTTWDGDSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVI
                     NAGDQRVTLANNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTT
                     DVDTRSATTGQGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLESVSTLTKSGGGT
                     LELTASGTATSAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSG
                     GDIDITDGTVLRLTQQDTNQVLDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYL
                     SDVTVNGNLTNTSGAISLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLN
                     GNTAGNTAVVINPITGIGEPTSTGIKVVDFAADPTQFQNNAQFRLAGSGYVNMGAYDY
                     TLVEDNNDWYLRSQEVTPTPPPDPDPTPDPDPTPDPDPTPDPGPTPAYQPVLNAKVGG
                     YFNNLRAANQAFVMERRDQAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGN
                     LFSGHWGDDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQK
                     QGVWLDSWLQYAWFNNDVSEQDDGEDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVI
                     YQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWSTAVGVTPTLDLNYYHDPHATEI
                     EEDGSTISDDGVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW
                     "
BASE COUNT        10373 a        10819 c         9865 g        10021 t
ORIGIN      
        1 ggcagcataa aatcatcctt ttatgaaaat caacgttttt tgagcactcg cacacaaaaa
       61 attaccgcac gggttgcaac gacaacctgc attttgcgga gcatatgaca aataaagtac
      121 gggtgcgttg aggatgcctg acacatcaga ggtggcggga gattactccc ccgcctggtc
      181 tcttacttct cagattcgta gtctacgaag acagcgacct ccgtctggcc ggttcggatc
      241 cgtacctcgc agaggtcttt cctcgttacc agtgccgtca ctatgacagt taaacagatg
      301 acgatcaggg cgaattaaca tcgccttttg ctgcttcata gactgcttct ccttgccttc
      361 cggcacgtaa gaggctaacc tacggttgtg tgacatagat tgggcctcag attaatgtta
      421 agcgtcttgc aggacacgta atgttaactg gggcttttct ccgtctgcct tacggcagca
      481 tgcccgaggc agacaccctc aagcacccgc agtcattcta cagtaaaacc ccgcgctttc
      541 aatttcccgt atgtccggaa aactgactga cctgtgcccc tgatcgttca cgctcaacgt
      601 atctgtccat ttccagttct attccctgca taatcaacat cccctcaata acaccttcag
      661 ctttttgcag tacccgcccg gcccagcaat cagagcgctc atgcttacgg cccagtccca
      721 taagcgtcat cccgaacaca taataatcat acagaaaatc atgcaaatcg ctgttgttga
      781 cgttcagttg cgccatgcaa ttgctgataa tcagcgcatc atcatcggta cactgaggcc
      841 gtgatttaac ctttgatggg atcactccct taaatccggc agcgacgccg gaccatgcca
      901 cactctcatg attatttacc acccatgcac cccatcggga cagaacctgt tggatatcac
      961 gcattattct gtactccgct gtaaccatac tttttctgat ttgaatgctc agggggaaaa
     1021 tgtctactgg atatctccgg cttcattgct cacactcccc aaccagattg agaatgaccg
     1081 ctgaaccatt gtcttccatg tactcatctt ttccgcttgc cagaaaccag cgacacactt
     1141 caaccgcttc atcacgcgtc acaggcttta tttttcccag caaattttcg agccagaact
     1201 gccgatcata tactgactca tgatgttcac ctgtgaaacc gtatttgtcc cctgtttctc
     1261 tccccgctgt ctggcgcatc tcataaagcc agtcccagta aataaactca cgaacaacgt
     1321 ctgacaatgt atgaggctct ggcagtacat cacgatagcc atcaacatat gcacgacgct
     1381 gatcatcaat ttcatttgag cgaacaccgt caatacttcc ggcttttttc tctgccgcag
     1441 tccagcccca gtagtaatcg tcgataaatt tcggggaggt gcgaattatc cgctcagcct
     1501 ccacatcgtc gagtgctgcc tcatagctgc caaatgtggc cctgactgat gcggcttttt
     1561 tgatattctc acgggcattc ctgatcgcct gcgccgggtt atctatgcct atggttgcga
     1621 aagcaatctg gaaaggatca acattaatct cctgcaaata acgtccatac cgttcactcg
     1681 cttcttccgg cgttattttg agtttctcca gtgctgcttc ggctgcgtcc agatgtacgg
     1741 cctcgttcag gtgaataacc tccagaatcc agagataagc atcagtctgt ttatgtccgg
     1801 tgatcctcct ttgttctggc agaggtttga tatttgccgg aacggcgctg tacgctgccg
     1861 tcgggatagt gaataaagct ttatgtacct gattgtccgt acgcatcacg caaccgcctt
     1921 ttttctgtaa aaaaccagtt cccgaacctg atcgccgttc atgagcatat tgttgaaatc
     1981 atcatgatca ggccaccgga cacttacccg ctgaatgtca tttcgggcct ggagatttgc
     2041 atgtgcgcat tcacaggccg cagccattcc ggtagcactg tattcatcgc gatcggcaaa
     2101 aataatcaga tgggtgactc ccgccggtac gcggaacttt ttcataaaac cactgttaat
     2161 ggttgcccag gtatttactt tgtaaatctg atgtgcggac aacgcggttt cgataccttc
     2221 agctataccc agtgtactgg ctaccggaaa catcctgata gcaactgacc gggcatggtc
     2281 cagatagtta tcgtcctgta aagatttgag ccttctggcg ctgataccaa tgtctgcttt
     2341 tttagctcca tcaagcaatg tctgatgcag gtaacacagt tctcccttgt catcggtcgc
     2401 cagggcataa agagactgga acatgcgccc gttataattt tgctggctgt tgaagcgaac
     2461 tccctcaacc ggaagactga atatgccacg ggaacgaaga tattcagcac cagatgttcc
     2521 ctgtagcgcc gtgagccctg aaaatcgacg ctgaacgcgc gatctcaggc tttccgacgt
     2581 acagatcaca ggcggtttat ctctccggta ttcattcccc gtgatgcggt ctatttcagc
     2641 gcaaatttca tgaaacgctc ttttttgtgt cagagcgatc agtttcatgc cgtcaccgct
     2701 accacaaaca cagatccacg ttccggtgcc atcacggtca tcaatacgga acttacctct
     2761 ggcaccacaa accggacatt cccctttgaa gtgcttttta cccgtgactg gcggcagtcc
     2821 gtaatgtttt aaaatttcag gccagtggcc tttcgctgct tctgctgttt tcatcttact
     2881 gacccagact gtttctgttt ttctgaagtt gttgctttgc ctgcatgatc tgccacgact
     2941 cactgccttc cggcacccca gaccggacac catgaagcag ggacagagaa agcgcttcgg
     3001 gggatttttc cgtcatcttc tctgcccggc tacgccctct ggcaaaccgg atgaatttgt
     3061 gtctgatgta attactgacc tccggggtga tttccatcgg aaaactgctc agcccgtcag
     3121 gccattcgcc gaacttctcc cggaaagtgt gagcgcacca tccatcgctg accggtcgcc
     3181 ccagagaggc acgctgacgc tgatagaatt tgatctgact ccaccaggcc tgtttgtcgc
     3241 ttttggtgta aattttttcg tccttgctga gtttcctgat attgcggcgg gtatctgtgt
     3301 tcacatcctc accagccagc ggtttaaatc cgcatttcgg acagacataa acacctgccg
     3361 gtttcatgaa atgacacccc ggacattctt tcggcagttt ttcttcccga atttcgttac
     3421 cacgtgatga tgacacctgc atcccgtcgt ttttgccggg cagttcgtca tactcaatat
     3481 tttcaggaaa tcccaggcga tgaaccgtac cggaatggtc gaaaatcagg catgactcct
     3541 tgcctggcgc tgtacgcaat cctctcccaa tcgcctgaag ccagcgaatc tctgattttg
     3601 tcgggcgtgc gtaaatgatg caccggacat cactgtcaaa tcccgccacc agaacgccaa
     3661 cgctgacaat gattttcgtc gctcccgttt caaaacgact gatcattaac tgacgctcat
     3721 catgtggcgt ttcagcaacc atcacctctg cgttaattcc ggctttgttg aactgcatgg
     3781 tcacgaagct ggcatgcgtt ttattgacgc agaacgccac tgtcggtaaa tcccttccgt
     3841 tacacagcca gttatcgaca atatcgccaa ccagatccgc gccgcacata atctctgcca
     3901 gttgtgattc gtcgtaatcc ctgccgtact ccattgagac tttcgttttt acaccttcca
     3961 gatccggttt cgtgggcgca taaaattcgt aattgctcag ttcgccacgc tggatcagtt
     4021 cccctatagt ggtcggcttg agaagacgct ggtaataacg ccccaggaac gatgaaaagg
     4081 gtgtaccgga caacccaatc accttcacat ttccctctgc aaccagtcgt tcgatttccg
     4141 tgaggatgcg gcgcttacgg agatgcgctt catcaataat cagcaggtcg atattctgag
     4201 gaaaatcacg gcgaatcagc gtatccgcac tggcaatctg aatcagtcgt gacgggtcgt
     4261 tattgggatg atcacgccag ataaagctga tttcatcccc ggaaagcccg tactgaacaa
     4321 agcgctgtgc tgtctggttg atcaaaatgg tataaggaac gacaaacaga acgcgcatgc
     4381 cacggcttac cagaccagca gtgatgaacg ccgccaggcc agttttacca ctccctgtag
     4441 gcatgtacat catgaaggac tgatatgttt tccagtcatg acgcaacata ttcagcgcac
     4501 gttcctgagc aaaattcggc ttgatattca gcatggtatt ctcccgatga cagggctgat
     4561 acagtgatta cttaaaccgg gtcgcccgga tgacatccac accatcggcg atataacgct
     4621 gtggatgttt gccatcacgg agaaaaacaa atcccgaaat gccttcagga agcgctcgaa
     4681 ttttcatcag tgaacgctga cgaggcgatc ggttatccac ctcaactgcg caacgctgac
     4741 catcgggaga aataaccaga agatcgataa atcccttgcg atcacctttc agcctgacag
     4801 tgaaacagcg ttgcaactca cagcccaggg atttgatttt cttttgcaga acgtagtcga
     4861 atgtgacctt gtccattcca tgtccgaatt cctcttccag aaggttgatt agtaccagtt
     4921 tcagttcagc actcatattt taaccatgat gtactagttt gctgatgcag ccgttttttc
     4981 aggggtgccc ccctttagat cgagatctac ctaacctctg tacccgtctg ttggaagatc
     5041 ccgttccagt gcttcgcact aacacacggg cactcccccc cttacccccc tctcaactta
     5101 tgtactagct tggtagtaca caggtgcatc ctgagaagtg gggtctcagc ccgtcggtca
     5161 cctttaagac caacaccatt caggtacgct gttgcgttcc tgccaggggc ggctgagtcg
     5221 tatacccctg taatgcacgc ccgtgtgttt caacgaacct gcgcagccgt acattggctt
     5281 catgcctcgc tctgttctcc ttacggaatg aaacaggctc ggaatcaaaa gtaacttcgt
     5341 aaacctccgc gtatttcagg gcgaccttct gtctcagtga ggaaggcagg ctctgtaact
     5401 gccgcttaat ccactctgaa tctgcctggc tgtacctggc aggcatctcc atctgaacgt
     5461 aatcagggga catacagcct ccggcagaac atcacgattc atgactggca ttcatgagcg
     5521 acggatgctg ataaggaatt ttgggatcca gatgacaaag gatcgccaca tcttccggca
     5581 cacctcgggt tttccacttt ccaacgccct gactacctct aggttttcct ctcttaggga
     5641 aacggcgacc aattgcagca ttggttttaa actcagcttt taaaatttcg taaagagtca
     5701 tctctcactc actgattgat actttagcgt ccataaatgt acaacaaatg aatctaaagt
     5761 atcaagaata tgtgctactt tagtatcgac cgaaaaaaca ggagagataa aatgagaact
     5821 ttgggagagc gccttagcag cgccaggcaa cgggcaggat tgacacaaga tgcattagca
     5881 aaaaaagccg gtataacccg cgtggctata agcaaagcag agcaaggact tactaaaagc
     5941 ttcaacggag atactttatt caaggtagcc acagccctaa actgcactcc acaatggcta
     6001 caaaatggag aagaacaatc tgtaaactgg gaaaacaatg ttaagagttg tcctcaaaaa
     6061 gacgcatttc attcctatcc cgttattaac tgggtccagg ccggactatt ctctactgct
     6121 ggagatgatt acagcatgta tgaccatgat aactggagac attctgtcaa atatgcaggt
     6181 gaaagaggtt tttggcttga agtacatggt gattcaatga cctcgcctgt tggaatcacc
     6241 tttcctgagg gtatgtctat tctcgtaaac ccagacaaag ctgtacattc aggctgttac
     6301 gttatcgcaa gaaaaaaatc aaccaacgaa gcaacattca aaaaatatgt gactgaaatg
     6361 ggaagatctt tcctgaaacc actcaatcct cagtatccca tcattgaaat agatgatgat
     6421 tgtgaaatcg tcggcgtgat cgtggatgcc cgatgggata ttttctaaga aagatacttt
     6481 agtatcaaaa acgcttgccc atgagtgata ctttagttac catactaaaa aagtaactga
     6541 ggtatcatta atatgttcca aaaaactacg ctatcatgca ttgaagattt aaaaaatctt
     6601 ggctccctca ttgctcttat cgcaaaagca gcgccagata caactcttgc caatgatatc
     6661 gagtcatgcg ccggaatggc gtgggatata ataaacaaag tatctcacac attatctttg
     6721 ttaattcata aacaaaacat ttctgattgc ccccatgaga tccgcaccca acgcgaaagc
     6781 tgcggtctat ccaccgctga actcgccacg ctgcttggag ttgatgaaga aaccatcatc
     6841 cagtgggaga acggcaaggc tgaacctctg gcaagccagg tgattcctct ggcaaatata
     6901 cttgactgcg atccgatgtg gttacttaca ggtttccatt ctgacactac aaaggaatcg
     6961 taagatgaaa atgttcaaag gcctcaccaa cgaaccggaa acagcttttc atcacattgc
     7021 agtactgctt gaagcggggt taatcatttc ggcttccggt gatgaagaat gtgatgaact
     7081 ttcggatgac atctttttac tggcacaaca atacgccaga agcgcatgcg atgcatttaa
     7141 ggaacaaaga acatgaaaac tcccctgatt atgcttgagg aagtggcagc agaaataaaa
     7201 gaaaacacgt caatgcttga gtttatattt aaaaactctg gtgataacgg agagacagat
     7261 gatttcttac tttgtctgat tcgttccatg aataaaacct gtgaaaaggc ttacgagtat
     7321 gttgatgctc tgagaacgaa taaaagaaat taaacagaca atcatacata aaaacatgac
     7381 ggtctgcggt cgggagttct cgctatttta aaatggggat gacacatgaa agacaaacat
     7441 gcctataaag ccgctttatt aatggctgat gccggatact ggtcagttgc agtattgttt
     7501 ctcaaaaagg catacgggaa ataacaaatg aaagatgaat acatttctgc atatcgtcgc
     7561 cgcataacgg ttgcagcact gcggcggatg aaacgaaaaa caggaagtaa tctcctcact
     7621 gtaaagcgtc atgatggaga gttaacaact attgaaataa cagaacagtt cataagccag
     7681 ttgctattac gatttgaagg cgttacccgt ggtgagtttg ggcggaaaga tggtgaaaca
     7741 gcaatacgca ctgcatacca ggatgctgtc ggtatcaacc aacatggtga gtatctgact
     7801 gaatcaggaa agttaatcat tgatgaactt ttgaatgaat gcatcgatta cattaaagag
     7861 gggcatatta ccggatgcgt taatcattaa cagcatagct cctcccccgg aataacggag
     7921 ataatgaaaa atgaataatt gcgttgaagt actcgataat gtccactgcg agataacaga
     7981 aagcctgtct ttgttacagg tgattattga cagcggcgat attggttcac cagatgcgct
     8041 aaacatgatg aattgtcttt tccgctcatt atcctgtaca gcagacaaag cagaaaagca
     8101 tctgtgccgg actgaaaacg aataacatta tttaagaaca gtaattaatt tacagcttta
     8161 ttgtcgggga tttttacaac ctgaaaaggc caggctgtat cacagatata tcgcaacaaa
     8221 cgaattgctg agccacatct tctggctgcg ctcagagtga ataaaagtaa ttaacaccgg
     8281 atgaacacgg agtaaccatg aaagaagaac ctgaaatcat tgaaattaat tctgaagaat
     8341 tcgcagaaaa cgaatggctg acagaaaccg atccgcgcgt tctggcgctg acaatggaag
     8401 gatacatcgt atttccacct ttcttcagtg atgcggaata cagtgcaata aataatgccg
     8461 gaatcgaccg tgcttttcat aaatacgaac tcgattgcgg ccctgtttac agagtctgcc
     8521 gccacggaca agttaagaaa tgcttcacgc gaaaaacggc catcatacat ctcgctcatt
     8581 ttatgacaac gaaggtattc gaaagttcag gttttgaaaa acgtctgcct gaccagcgcc
     8641 tgatgcatcc ggaatatgga gaagtctttc atccgggaga aattacgccc gaatactaca
     8701 gagctcacgg gcgttgtttt catcgcctgt tgcgcatcct tgcacgcaga aaacagtttg
     8761 atgcctggat ggaaaaatac aacgcctgga ttgatcagta ttgccggctt gtcgcgcaaa
     8821 gaccatttaa gaagagagca aaccatgact gcaactgaac agttattttt tcttaccgca
     8881 gaaggtatcc acacacctta cggactgctt gatatcaaca aagcactgga aatgctcgat
     8941 gaaggctgtt ttgacgagcc gatctggcat ggtctggaaa tagtgtgttg catcagtgaa
     9001 gcagaagaaa aaggcatgac aggtgagcta gcgccggaaa agacaatcat gtactggcgc
     9061 tggctttata ccgccatgtt cttctctgaa cagctcacca gaaacggctg gaccgaagtg
     9121 cttaacgaga atggcggtac agacaagggc accatgtaca gaggccacaa cggtggcatg
     9181 acaatctacc tgaaaccatt acgtctcgct atgcagaact atatggaatg ggcactcata
     9241 gaaacttacg gagcggaatc agggctgctc aaaagcattc ttatgtacaa agcattcacc
     9301 ggcgctctgg gcgacggcga aaaaaccggc atcactatgg aaggttacag agcgatgcac
     9361 atgattctgg atgggcttat cagtgaagcc actgacaact cagaaccgga aaccagagtg
     9421 ttccactaat cagcttttcg tgaggtgtaa tgatgaaaaa atcaactgaa cttgtgccgg
     9481 tggcagcccg tgatttgcaa attatcgaat atcgcggtca acgcgttgtg accactgaac
     9541 aactggcggc ggggtatgga acaacaccaa tccgtattca gcagaatcac catgaaaata
     9601 aatcccggtt tattgagggg aagcacttct ttaaagttgt tggtgatgaa ctcaaaaatt
     9661 tgcgactagt tttaaacgag tcacaaaatg agatgaaaat atctcccaaa actcgctccc
     9721 tcatcctctg gaccgagcgc ggcgcagcca accacgcaaa attactggaa actgatcagg
     9781 cgtggaacta tcacgaagac cttgtggagt tctacttcac tcagcgagaa aacatcgatg
     9841 ttcagcgcgt actcactcgc aaggaactcg cgttgatggt tattgaggct gaagaacggg
     9901 ctgaagctat ggtcctggaa aataaaacgc tctctgcaac cgtagacagt ctggaaaaac
     9961 atttctctaa aggcatgact gttccccagt tctgccgtgc gctgaacggc gtaaatacca
    10021 gccagataat gtggtggctg tctggccgaa aatgggtatt caacgaacaa cgcgatccgg
    10081 agaaaacacc ccgctggcgt acggcctctt atgcccgtga cagatacctg accgaagaag
    10141 cagtacagat cactccacac gggaaagcaa cattcacgaa attcacaccc gtattgctgg
    10201 aaaaaggatg ccaccgttta taccagtttt acatgaacgg tgaactccct atgaagaaaa
    10261 actggaacgg tgaatttaaa cacgacaaag ctatttacac accggaggca gaacatgaat
    10321 aacactactg cggcaatcat gaactacgac ccgaacatga cattatgcgg gcgcatggca
    10381 agacagacgg ttcgtctgac actcgggcaa tgggaatacc gcgaaacatt tgaagtggac
    10441 gttgtcggca atctgacggg actggatgta ataacctgcg caattgaaaa cctttatgaa
    10501 gacctgccgt atgaagaagc acagaatgaa tataccggtg aaactaacat tatggccaca
    10561 atacaggtcg gtgatcttgc atgtacggat gagggcatgc tcggcgaagc atggctggca
    10621 gatatgctta ttgcagcaga aattatccgc attgaaccag caggaacact ttcataacgg
    10681 agatcgccat cagatgatta tttcattcac cgattacggc gaacaggcaa aactggttat
    10741 caccagttcc cgcctgacat tgcgaaaaca taatcgtgtc gttgatgcgg tacttctgtg
    10801 cacgccggac atcacagaaa gccggagcgg ttttttccta gttaagtcgt cattatcagg
    10861 aaaaacacgc gacatcctgc gcgcctacaa aacagcaaca agggagtctg aacgatgaaa
    10921 gaagctgatc tgaaagcact gcgtgacgcc gcacaaaata ccgtatatgg cgacaaggga
    10981 tgtgatctgg aagcatttca ctcccttgcc actccgccag ttatccttgc gctaatcagt
    11041 cacatagaaa atctggagaa aaaactggat gaaatcgtaa cgtcagagat tagcccgaag
    11101 gcctggctga ataaccttac gggggaagtt tgcagtattg atgccatgcc tgacgcccgt
    11161 aatgacagag aaacatacac cccgctttat cctgcttggc agcgcattgc cgccgcagat
    11221 gataacggca gagtggcatg gatgtatacg cacaaaaaag caggttacag ggtccccaca
    11281 atgacatcgt gggaaagtct tgcagaagag cggggaggct ctctggataa atatatcgtc
    11341 agtccactat tcactgttcc tcagataccg ccagtgagaa cagaacagga tgcttaccct
    11401 gatgtgcagg tttcatggga tgcaacacgt cactatgtca acggctggaa cgcctgccga
    11461 caggcaatgc tgacgacggc agaaaaagta cttgatgtgc gaatccctga atatatcgga
    11521 cgctggcttt ccgctgcact tcatgatcag aactcatgcg aggaaatgaa aaacgatata
    11581 cgacgcttcc tggctgaata ccaccatgtt ccatcgaaat ataacggctg gcgacttgta
    11641 ccggacaagt taaccgaaca gatgaagtac cggattgtgc cactactggc gaccatcaag
    11701 ccccgcagcg tcgatgaata tgaccgctca ggagaaaacg cacaatttct tgaatgggta
    11761 tggacgcaac tgatggaagc cacgaaattc gcgtatgaag gctcagtgaa tgggtgaaat
    11821 ggttgatatt aacgatgaac tggtccgcac agtaattacc gttgatgacg gatgcgacca
    11881 tacccagcgt tttatctgga tgatgaatac acggcgtaat atcagaaaca aagtgttccg
    11941 gcacgaacca aagccagtca gggtgacaaa gatacgcatt gaagccagaa aacgccgcaa
    12001 atacaggcca cggaaggcca gggccccagc gcaaccgaat gaactggcat aaagacagca
    12061 ggagaataag tcgtgaatcg cgagtttgaa atatggatac gactcagata cgggtaccga
    12121 tacgatttaa ccagagatgc tcacggcttc tactgccgtg aagttgtaaa gcgaatgttt
    12181 gacgtgtggt gccactgccg tggcctgaag atagtgtgag tcaggaatgg aaacaatcat
    12241 ctatcagata acgcccagca aatggtgcac agaaagaacc cttattgctg caactggact
    12301 gaaatcagga accattgcca gagcaagaaa aaaatcctgg ttactgggga aagaataccg
    12361 tcattacgca gcagaaggag agcccgggcc tttcagtgaa tgtgtataca acattgagga
    12421 aattatgcgc tggatcgaaa accagaaaca accaggtgcc aaaaatgcaa gttccggtta
    12481 acctgttaat gctcctggac gtctgggagg tttaatgagt aacgcatcat acccgacagg
    12541 cgttgaaaac catggcggat cactccgcat atggtttcac tataacggca aacgtgtcag
    12601 agaaaacctc ggtgttcctg acaccgccaa aaaccggaag atcgcaggtg aacttcgcac
    12661 ttccgtttgt tttgcaataa gaatggggag cttcgactac gccgcgcagt tccctaattc
    12721 ccctaacctg aaacattttg gtctgggaaa aaaagagata accattaagg cactttcgga
    12781 aaaatggctt gagcttaaga aaatggaaat aacagcaaat gccatggtca gatacaaatc
    12841 agctatccga aattgccttg actgccttgg agggaacaaa ctggcatcat caattacaca
    12901 ggaagatgtt ctcatttttc gtaaagaatt attaacggga tatcagttgc caggaaaaaa
    12961 tcagaggaag cctattaaag gtcgtagtgt acctactgta aatagctata tgacaattat
    13021 ctccgggatg ttccgttttg cggcaaacaa tgggtatata ggcaaaaacc ctttcagtga
    13081 gcttccatca ctgaaaagag cgaaaacacc tcccgatcca ctaacaaaag atgagtttgc
    13141 cagactgatt gatgcgtgcc accaccagca aactaaaaat ttctggtcgc tggctgtata
    13201 taccgggatg cggcatggcg aactgtgtgg gctggcatgg gaggatatcg atctggaagc
    13261 gggtaccttg accgtcaaaa ggaattacac tcagacaaag gaatttacac tacccaaaac
    13321 agatgcagga acaaacaggg taatacaact catacagcca gcaattgacg tattaaaaaa
    13381 tcaggcgaca ttaaccaggc ttggtaaaca atatcagata gaagtaaagc tgcgagagta
    13441 tggccggaca agcactcact catgcagctt tgttttcaat cctcagatat tagatcgcac
    13501 aggtaactca ggagttcatt atgccgtaac atccatcaac agaacatggg aatctgcatt
    13561 aaaacgagca aaaattcgcc atagaaaagc gtatcagtct cggcatactt atgcttgctg
    13621 ggctttaact gccggagcaa atccaaactt catagccaac cagatgggac atgcgaatgc
    13681 ccagatggtt tacaatgttt acggtgcatg gatgaaagac aataaccagt cccaggtaga
    13741 agtactaaac agaaagctat gtacttatgt cccacaggtg ccccaacgac taaacaaagc
    13801 atagaatata tttttaaaat caaatagtta atacataggt gcaagtacat gaacaatatg
    13861 aacgtaatta ttgccgatga ccatccgata gtcttgttcg gtattcgcaa atcacttgag
    13921 caaattgagt gggtgaatgt tgtcggcgaa tttgaagact ctacagcact gatcaacaac
    13981 ctgccgaaac tggatgcgca tgtgttgatt accgatctct ccatgcctgg cgataagtac
    14041 ggcgatggca ttaccttaat caagtacatc aagcgccatt tcccaagcct gtcgatcatt
    14101 gttctgacta tgaacaacaa cccggcgatt cttagtgcgg tattggatct ggatatcgaa
    14161 gggatcgtgc tgaaacaagg tgcaccgacc gatctgccga aagctctcgc cgcgctgcag
    14221 aaagggaaga aatttacccc ggaaagcgtt tctcgcctgt tggaaaaaat cagtgctggt
    14281 ggttacggtg acaagcgtct ctcgccaaaa gagagtgaag ttctacgcct gtttgcggaa
    14341 ggtttcctgg tgaccgagat cgctaaaaag ctgaaccgca gtatcaaaac catcagtagc
    14401 cagaagaaat ctgcgatgat gaagctgggt gtcgagaacg atattgctct gctgaattat
    14461 ctctcttctg tgacgttaag tccggcagat aaagactaat cacctgtagg ccagataaga
    14521 cgcgtcagtg tcttatctgg catttgcacg gattgccgga tgcggcgtaa acgccttatc
    14581 cgtcctacga ttcccgcgat ttcctgaccc tctcggcata taccgtcagc gtctgtttta
    14641 tcacatccag cgttaccggc ttcgacaggc agctgtccat accggactcc agacaccgct
    14701 gcttctcttc agccaacgca ttagcagtta ctccgattac cggcaacgtc agtcccaact
    14761 gacgaatgcg ttgcgtcaag cggtaaccat ccatatttgg catgttgacg tcgctaagca
    14821 cgatatcaat atgattcttg ctaagtacat taagcgcatc gacgccatca ttcgcggttt
    14881 tacattgata gcccaacgat cccaactgat ctgccagcag acgccggtta atcggatgat
    14941 catccacgac cagaatcatc atatcgtcat tatcgctgac cgctttgtcc gttaacggca
    15001 gagcgttagc aggatcgtcg ctctccatct cgatcaaata aatacgcgcc aacaatgccg
    15061 gtagctcatg cggggcagcc acactgtgta cccactctcc tggcgctttc tccagcggaa
    15121 taccaatatg gcgacgacag aaggtcacaa ccgctctacc ctgccatttt ttgctcacta
    15181 cctcgtcagt gattaacaca tcttcgggag tcggttcctg cccttcgtat gttgtaacga
    15241 cgatgccgct gcgctgcaaa ctggtttcca ggaactgaca gagcgacgca ttgcggaccg
    15301 ccagccagca acgtttacca ctcaaccctt ccacgccttt tttctgcggg tactgagcgc
    15361 cgtacaacgg aatacgcacg gtaaactggc tgcccattcc cggttctgaa tctaccgaga
    15421 tgtcgccgtc catcatgctg atcagttttt cacaaatcgc cagacccaga ccggtcccct
    15481 ggaagttacg ctgtacgccc gttccgacct ggaagaaggg atcaaacaag cgcaccactt
    15541 ctttcgccgg aatccctacg ccggtatcgc gaacacggat agagagataa tcgccatcag
    15601 cgcgaacatg cagaactata cagccggtat cggtgaattt tatggcgtta ctcaacaggt
    15661 tggagatgac ctgctgtaaa cgcatcgggt cgccatttaa ggccactggc acatccggtt
    15721 caataaagca gtacaagcct agctgcttgc gtaccaccag cggtaaatag ttggcggtga
    15781 tgtggttcat cacttcacgc ggtgaaaact cacgcggttc gatcttcaac tgttccgatt
    15841 caatcttcga gaagtcgaga atatcgctga taattttcaa caacaggctg gaagagttgt
    15901 tcattgccgt caccagacga tcgacgcctt tcggtaactc tttggtttgc aacagatcca
    15961 ggttaccgat aatgccatac agcggcgttc gcagctcatg actgacggtg gcaaggaaca
    16021 tcgattttga ctggctcgcc tgttccgctg cttgtgccat ctcctgcaac gactcttcca
    16081 tcttcacgcg cgaagaaaca tccaccagca cacaaatggc cacgttttca ttacgatagc
    16141 gcgaatggac gaagctgatt tgcaggttgg tattgttgct ggtcaggaca tcaacaaaat
    16201 tgacctgctg cccacagata atttgtgtca gccgttggcg gtcctcatgc gtaagcatat
    16261 tgagataggt atgcgccagt tcgttactta aaatattgac gccatcagcg gtacgcaaaa
    16321 tgcagatacc cactggcgcg gaggcgacaa tcttgcgatt aaactgctca tgttcttcca
    16381 gtcgcagggc gtcgctttcc gccggaatga aaatacgccg ctcgtacatc cgcgcgagag
    16441 taaacaatgc agctccggca agcacattca gcaaaattgc gttaaggatc aacatgcgaa
    16501 tgcgttccag caccttatca accggcaccg aatacacgat gcttagcgat gagggtggca
    16561 gatttttctt cagcaccagc tcccggaacc cttccgtata gccaaaccag gagcgttcct
    16621 gcatccagcg aggatcgccc ttaattttgc tttctggtcc ggtaagcgaa atcagggtat
    16681 gaccattttc atcaagaatg gtaaccccca tcggcaacgt accaggtaag aaaaagttct
    16741 ccatccggat ggtctgctcg acacccaaaa gcgcctgcaa ccggttcgcc agataaactg
    16801 gcgtcaaggc gtaaaaatac ccgacgccag ggcgcggacc ttcgctgatc cagtagaggt
    16861 tactgccact atcatcttgt ggtgcatttc gatatttatt aatgcgttca tgcaaagctt
    16921 ttaacgcggt atcgcgttcc actggcatat cacgcagacc gaaattggcc atgcagaggt
    16981 tatcgctgcc gattaaaaat acccggttga gatcgtaagc cgcagaaaaa ttatcgcgcc
    17041 agtagcgcat aaaccacgcc aatgactcca gagaacctcg ccaggtgtta ctcattgcgg
    17101 aacaatcaga gtcagcaaat agcggttcaa acgcaggcac atccgtctgc gtttctcgtc
    17161 cacgcgggga aagcacaccg ttttctgccg ataagcgatt ttcagctatg tacttcagct
    17221 ctttcatcac atcagaggtg cgttgaataa agcgctgagc ctgatcggaa ctcagattaa
    17281 attcctgacg aatttccgat tctcgctgat gtaacgcatt aacgatgtaa aaaacggatg
    17341 aaaaagcaat caacagccag agcactaacg ccaatgctct gaacatgtag cgcgaggctt
    17401 tcagggttgt acgaaaagaa gcaaggtatt tcaaaggggc gaagctccgc ctcaggtgac
    17461 cgatggagtg tggttaaggt agcggtaaaa gcgtgttacc gcaatgttct ctcttctctg
    17521 gaatatgata caccgccgag aaatcatcac cttaacctct gataatcgtc atataccgga
    17581 caagactagt ggatttcagc atgcattata tgaagtggat ttatccacgc cgcttacgta
    17641 atcaaatgat cctgatggca atcctgatgg tcattgtccc aacgcttact attggttata
    17701 tcgtagaaac ggaaggacgt tcagcagtct tatctgaaaa agagaaaaaa ctttctgccg
    17761 tggtcaacct gcttaatcag gcgctaggca atcgctacga tctctacatc gacttaccgc
    17821 gtgaggagcg tatccgcgca ttaaatgcag aacttgcccc cattaccgaa aatatcactc
    17881 acgccttccc tggcatcggc gctggttatt acaacaaaac gctggatgcg ataatcacct
    17941 acgcgccttc agcgctatat cagaataatg tgggcgttac cattgccgca gatcaccctg
    18001 gtcgcgaagt catgcgtaca aatacccctt tggtttattc aggcagacag gtgcgcgcga
    18061 tattttgaat tcaatgattc ccattgagcg taatggtgaa atcctcggct atatctgggc
    18121 caatgaatta accgaagata ttcgccgcca ggcctggaaa atggatgtga ggattatcat
    18181 tgtgctcacc gccggtttgc tgataagcct gctgttgatt gtccttttct cccgtcgcct
    18241 gagcgccaat atagatatca tcaccgatgg cctctcgact ctggctcaaa atattcccac
    18301 tcgattacca caattgcccg gtgaaatggg gcaaatcagt cagagtgtta acaacctcgc
    18361 ccaggcactg cgtgaaacgc gaacacttaa cgatctgatt attgaaaacg ctgccgatgg
    18421 cgtcattgcc attgaccgcc agggtgatgt aaccaccatg aacccagcag cagaagtcat
    18481 cactggctat caacgtcatg aactggtagg gcagccttac tccatgttgt tcgacaatac
    18541 tcagttctac agtcccgtac tggatacgct ggaacatggc accgaacatg tagcactgga
    18601 gatcagtttt ccaggccgtg accgcaccat tgaactcagt gtcacgacca gtcgtattca
    18661 taacacgcac ggtgaaatga taggtgcttt ggtgattttc tctgatttaa ctgcccgcaa
    18721 agaaacccag cgccgcatgg cgcaagcaga acgcctcgcc acactgggtg agctgatggc
    18781 tggcgtggcg catgaagtac gtaatccgct aacagctatt cgtggttatg tacagatctt
    18841 gcgccaacaa acccgtgacc caatacatca ggaatatctg tccgtagtac tcaaagaaat
    18901 cgattcaatt aacaaagtta ttcagcaatt gctcgaattt tcacgcccac gccacagtca
    18961 atggcaacaa gtcagcctca atgcattggt tgaagaaact ctggtactgg tacaaaccgc
    19021 cggcgtacaa gcgcgggtcg acttcataag cgaactggat aatgaattaa gcccgattaa
    19081 cgccgatcgt gaactgctca aacaggtact actgaatatc ctgatcaatg ccgtccaggc
    19141 tatcagtgca cgagggaaaa ttcgcattcg aacctggcaa tacagcgact cacaacaggc
    19201 catttcgata gaggacaacg gctgtggcat tgatctctcg ctgcaaaaaa agatcttcga
    19261 tccctttttc accaccaaag cctcaggaac cgggcttggt ctggcgttaa gtcaacgcat
    19321 cattaatgcc catcagggtg atattcgcgt cgccagtttg ccgggctacg gcgcaacctt
    19381 cacgcttatt ttaccgatca acccgcaggg aaatcagact gtatgactgc tattaatcgc
    19441 atccttattg tggatgatga agataatgtt cgccgtatgc tgagcaccgc ttttgcacta
    19501 caaggtttcg aaacacattg tgcgaacaac ggacgcacag cattacacct gtttgctgat
    19561 attcaccctg acgtggtgtt gatggatatc cgcatgccag agatggacgg catcaaggca
    19621 ctaaaggaga tgcgcagtaa tgagacccgg acacccgtta ttctgatgac ggcctatgct
    19681 gaagtggaaa ccgccgtcga agcgctacgc tgcggggcct tcgactatgt tattaaaccg
    19741 tttgatctcg atgagttgaa tttaatcgtt cagcgcgctt tacaactcca gtcaatgaaa
    19801 aaagagatcc gtcatctgca ccaggcactg agcaccagct ggcaatgggg gcacattctc
    19861 accaacagcc cggcgatgat ggatatctgc aaagacaccg ccaaaattgc cctttctcag
    19921 gccagcgtct tgattagcgg tgaaagcggc accgggaaag agttgattgc cagagcgatt
    19981 cactacaatt cgcggcgggc aaaggggccg ttcattaaag tcaactgcgc ggcgctgccg
    20041 gaatcgttgc tcgaaagtga actgtttggt catgaaaaag gtgcatttac tggtgcacaa
    20101 accttgcgtc agggattatt tgaacgtgcc aacgaaggta ctctgctcct cgacgaaatt
    20161 ggcgaaatgc cgctcgtact gcaagccaaa ttactacgca ttctgcagga acgggaattt
    20221 gaacggattg gcggtcatca gaccataaaa gttgatatcc gcatcattgc tgccaccaac
    20281 cgtgacttgc aggcaatggt aaaagaaggc accttccgtg aagatctctt ttatcgcctt
    20341 aacgttattc atttaatact gccgcctctg cgcgatcgcc gggaagatat ttccctgtta
    20401 gctaatcact ttttgcaaaa attcagtagt gagaatcagc gcgatattat cgacatcgat
    20461 ccgatggcaa tgtcgctgct taccgcctgg tcatggccgg gtaatattcg agagctttcc
    20521 aacgtcattg aacgcgccgt cgtgatgaac tcaggcccga tcattttctc tgaggatctt
    20581 ccgccgcaga ttcgtcagcc agtctgtaat gctggtgagg caaaaacagc ccctgtcggt
    20641 gagcgtaatt taaaagagga aattaaacgc gtcgaaaaac gcatcattat ggaagtgctg
    20701 gaacaacaag aaggaaaccg aacccgcact gcgttaatgc tgggcatcag tcgccgtgca
    20761 ttgatgtata aactccagga atacggtatc gatccggcgg atgtataacg ccaaaacttg
    20821 ctatgcagaa atttgcacag tgcgcaattt tctgcatagc cgctcattct ccttatgaat
    20881 ccccatctaa tttattcctt tctattcaat tagttaaata acaacattca ataatctcat
    20941 tctggcactc cccttgctat tccctgactg tacccacaac ggtgtatgca agagggataa
    21001 aaaatgaaaa caaaattgat gacattacaa gacgccaccg gcttctttcg tgacggcatg
    21061 accatcatgg tgggcggatt tatggggatt ggcactccat cccgcctggt tgaagcatta
    21121 ctggaatctg gtgttcgcga cctgacattg atagccaatg ataccgcgtt tgttgatacc
    21181 ggcatcggtc cgctcatcgt caatggtcga gtccgcaaag tgattgcttc acatatcggc
    21241 accaacccgg aaacaggtcg gcgcatgata tctggtgaga tggacgtcgt tctggtgccg
    21301 caaggtacgc taatcgagca aattcgctgt ggcggagctg gacttggtgg ttttctcacc
    21361 ccaactggtg tcggcaccgt cgtagaggaa ggcaaacaga cactgacact cgacggtaaa
    21421 acctggctgc tcgaacgccc gctgcgcgcc gacctggcgc taattcgcgc tcatcgttgc
    21481 gacacacttg gcaaccttac ctatcaactt agcgcccgca actttaaccc cctgatagcc
    21541 cttgcggctg atatcacgct ggtagagcca gatgaactgg tcgaaaccgg cgagctgcaa
    21601 cctgaccata ttgtcacccc tggtgccgtt atcgaccaca tcatcgtttc acaggagagc
    21661 aaataatgga tgcgaaacaa cgtattgcgc gccgtgtggc gcaagagctt cgtgatggtg
    21721 acatcgttaa cttagggatc ggtttaccca caatggtcgc caattattta ccggagggta
    21781 ttcatatcac tctgcaatcg gaaaacggct tcctcggttt aggcccggtc acgacagcgc
    21841 atccagatct ggtgaacgct ggcggacaac cgtgcggcat tttacccggc gcagccatgt
    21901 ttgatagcgc catgtcattt gcgctaatcc gcggcggtca tattgatgcc tgcgtgctcg
    21961 gcggtttgca agtagacgaa gaagcaaacc tcgcgaactg ggtagtgcct gggaaaatgg
    22021 tgcccggtat gggtggcgcg atggatctgg tgaccgggtc acgcaaagtg atcatcgcca
    22081 tggaacattg cgccaaagat ggttcagcaa aaattttgcg ccgctgcacc atgccactca
    22141 ctgcgcaaca tgcggtgcat atgctggtta ctgaactggc tgtctttcgt tttattgacg
    22201 gcaaaatgtg gctcaccgaa attgccgacg ggtgtgattt agccaccgtg cgtgccaaaa
    22261 cagaagctcg gtttgaagtt gccgccgatc tgaatacgca acggggtgat ttatgattgg
    22321 tcgcatatcg cgttttatga cgcgttttgt cagccggtgg cttcccgatc cactgatctt
    22381 tgccatgttg ctgacattgc taacattcgt gatcgcgctt tggttaacac cacaaacgcc
    22441 gatcagcatg gtgaaaatgt ggggtgatgg tttctggaac ttgctggcgt ttggtatgca
    22501 gatggcgctt atcatcgtta ccggtcatgc ccttgccagc tccgctccgg tgaaaagttt
    22561 gctgcgtact gccgcctccg ccgcaaagac gcccgtacag ggcgtcatgc tggttacttt
    22621 cttcggttca gtcgcttgtg tcatcaactg gggatttggt ttggttgtcg gtgcaatgtt
    22681 cgcccgtgaa gtcgcccggc gagtacccgg ttctgattat ccgttgctca ttgcctgcgc
    22741 ctacattggt tttctcacct ggggtggcgg tttctctggc tcaatgcctc tgttggctgc
    22801 aacaccgggc aacccggttg agcatatcgc cgggctgatc ccggtgggcg atactctgtt
    22861 cagtggtttt aatattttca tcactgtggc gttgattgtg gtgatgccat ttatcacccg
    22921 catgatgatg ccaaaaccgt ctgacgtggt gagtatcgat ccgaaactac tcatggaaga
    22981 ggctgatttt caaaagcagc taccgaaaga tgccccacca tccgagcgac tggaagaaag
    23041 ccgcattctg acgttgatca tcggcgcact cggtatcgct taccttgcga tgtacttcag
    23101 cgaacatggc ttcaacatca ccatcaatac cgtcaacctg atgtttatga ttgcgggtct
    23161 gctgctacat aaaacgccaa tggcttatat gcgtgctatc agcgcggcag cacgcagtac
    23221 tgccggtatt ctggtgcaat tccccttcta cgctgggatc caactgatga tggagcattc
    23281 cggtctgggc ggactcatta ccgaattctt catcaatgtt gcgaacaaag acaccttccc
    23341 gatcatgacc ttttttagtt ctgcactgat taacttcgcc gttccgtctg gcggcgggca
    23401 ctgggttatt caggggccgt tcgtgatgcc cgcagcccag gcgctgggcg ctgatctcgg
    23461 taaatcggta atggcgatcg cctacggcga gcaatggatg aacatggcac aaccgttctg
    23521 ggcgctgcca gcactggcaa tcgccggact cggtgtccgc gacatcatgg gctattgcat
    23581 cactgccttg ctcttctccg gcgtcatttt cgtcattggt ttaacgctgt tctgacggca
    23641 accctacaga cagaaggaat ataaaatgaa aaattgtgtc atcgtcagtg cggtacgtac
    23701 tgctatcggt agttttaacg gttcactcgc ttccaccagc gccatcgacc tgggggcgac
    23761 agtaattaaa gccgccattg aacgtgcaaa aatcgattca ctacacgttg atgaagtgat
    23821 tatgggtaac gtgttgcaag ccggactggg gcaaaatccg gcacgtcagg cgctgctaaa
    23881 aagcggactg gctgaaacgg tgtgcggatt cacggtcaat aaagtgtgtg gttcgggtct
    23941 taaaagtgtg gcgcttgccg cccaggcaat tcaggcaggt caggcgcaga gcattgtggc
    24001 ggggggtatg gaaaatatga gtttagcgcc ctacttactc gatgcaaaag cacgctctgg
    24061 ttatcgtctt ggagacggac aggtttatga cgtaatcctg cgcgatggtc tgatgtgcgc
    24121 cacccatggt tatcatatgg ggattaccgc cgaaaacgtg gctaaagagt acggaattac
    24181 ccgtgaaatg caggatgaac tggcgctaca ttcacagcgt aaagcggcag ccgcaattga
    24241 gtccggtgct tttacagccg aaatcgtccc ggtaaatgtt gtcactcgga agaaaacctt
    24301 cgtcttcagt caagacgaat tcccgaaagc ggattctacg actgaagcgt taggcgcatt
    24361 gcgcccggcc ttcgataaag caggaacagt caccgccggg aacgcgtctg gtattaacga
    24421 cggtgctgcc gctctggtga ttatggaaga atctgcggcg ctggcagcag gccttacccc
    24481 cctggctcgc attaaaagtt atgccagcgg tggcgtgccc cccgcattga tgggtatggg
    24541 gccagtacct gccacgaaaa aagcgttaca actggcgggg ctgcaactgg cggatattga
    24601 tctcattgag gctaatgaag catttgctgc acagttcctt gccgttggga aaaccctggg
    24661 ctttgattct gagaaagtga atgtcaacgg cggggccatc gcgctcgggc atcctatcgg
    24721 tgccagtggt gctcgtattc tggtcacact attacatgcc atgcaggcac gcgataaaac
    24781 gctggggctg gcaacactat gcattggtgg cggccaggga attgcgatgg tgattgaacg
    24841 gttgaattaa tcaataaaaa cacccgatag cgaaagttat cgggtgtttt cttgaacatc
    24901 gacggcgaag gtaaccccat taatcaccag tcaaaacttt tcaccagcgt cagctcgcca
    24961 gcattacgca tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg
    25021 atcagcgtca gttgggcggt cgtcaattcc gtttcgctgc gtccgccata gtagttgata
    25081 tacacctgat aacgtccgtg aatcggcgct ggcatggcga aaatctcggg tccgtacccc
    25141 gtcgtgacat ccatatccag tgcaccactg tttttcagca ccgtgttacc gtaccaggcg
    25201 tgttcgccat cgggcgtgac aacatggaga tcgagatcgg tattgtccgt atcccacgag
    25261 agaaccagcc gtaaacgtgc acgaatcgtt cctgtacccg gcgttgagta aaactgcatt
    25321 ttttgccggc tttgcccatc cgggctgatg acctgcacgc tgttgctgcc ttccgtgaaa
    25381 atataagggc gtgcaaatga accatcggat tcaattctct gcggcatact ggcaccgttg
    25441 accaccagcc ggccctgctg aactttaccc gccgcagcat aattctttat tttgccgcgg
    25501 atctgcgctg agatattttg atcatctgcc atgttgacgg acgacgccgg gtaattgatc
    25561 gattgtgaaa aactggcatc ttcaccctct gccggatgcc agccagagag cggtgcatca
    25621 atctcgactt cctgcacacc ttcactgtga gcgacaggcg aaagcgccac cagtaacaac
    25681 ggaagaaaaa tctttcgcat agcattagtc cattaataat tgtcgggtaa gcgtttcgat
    25741 atagttctca tcaagcccgg tgggataacc atcaaaggcc aggtgcagat actcatgggt
    25801 taaatccagc cgatcctgaa acgtaaagaa gttacgaata aacagccgtt tctgctgccg
    25861 atcggtatag gggaaacctg agactaaacg gcaaaccgca aacacgtctg gttcgttgta
    25921 ccccgtctca ccttgtaata tacgccgcca ctgcggcatt tttttcgcca gccaggcttt
    25981 cgctttgggt aataactggc aggttgagcg tggcgctccc cagcggctaa ggctattgtc
    26041 gggataagca aacgcgagta tctgatcgta acgctctccc tgccccgcct gggccgtggc
    26101 ttgccgccag gaaagcgtcc cttcggtagc gcgactgccg tgataatgaa ccggatcgcc
    26161 cgcatagata agatcctgcg tccaggcggt catcgttcgc gcccccgtgg tagctggcga
    26221 agcagaaaca cgctgcgtgg cgctgctgtc agggatggtc aggcaatcgc cttcccgatt
    26281 ggcgttttgc tgtaaaaaag tgcgtatcgc cacggtcatg gctttcgctg cctcaggtgg
    26341 cgtacttttc gcctcacgat ccagaactct ggcaacgtac tcttcacgat cgagatgcga
    26401 ctgcaatttc agtttgccct tttcgctcag taaggtcgtc tcaccgtggc tgacaaacat
    26461 aatgtggtta ccattggcaa acgtcacacg atagcgccca ttaagtacgc cgggttttac
    26521 tgccgtcgaa ctcttttccg ccgtgatttt cttcagcgga tagcgggcaa acagctccac
    26581 caggacacat tgcccactgg caacttgcgt cggcactggc aaaacccgat taagcaccgt
    26641 cgcataacgg gtcagtaccg ttttgctggt gccactgcca gtcacccaca gcggtgtacc
    26701 atcggttaac cagccggcaa aaccaccctg acggatttcc tgtttatcat cggcaaacca
    26761 gctccaggtt ttcacccgta cccgactgcc gagcatggat gcgacaccga ttttcgcctc
    26821 gtccagcacc acatccagta agacttcctg agctttgttt tgcgcaggta acgtcgctaa
    26881 tgaatcaagc agcgagttaa ccgttaccga ggtttctggc ttcagcgtag taagagacgc
    26941 taaccacgca ggtgcctgac gctgttgcca atattgcccc cacacatctg cgccaatatg
    27001 caaccgctgc ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat cgcgcgttat
    27061 actctcccca gcctgacaac aataaacctc ttccggggag tttccgcgac actggtaggg
    27121 ctgctccggt tgatgggtat cctccagcca ggcgtaaaca tacagtttcc acaaactccc
    27181 taacggcgtg gtgagcgtgt caggtaatgc cgagactttg gtcacgcctg atgacgacag
    27241 ctgatacagt tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc
    27301 aagcgttggc agagtaacca acgccaatag ccagacgatt ctgcgccagt tcacttattt
    27361 cacctgcatc ccggtccatt cgctccctgc cgcaacactt tgctgtgcag gtgcatagga
    27421 acgcacataa cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg
    27481 gcggaaagtg accgttcccg tcagttcttt caccggcacc atataggcca gtttgcccat
    27541 ttcatttcgc gctttttcca gcaactgccc ctgctgtttt ccagcgttcg gtttattgac
    27601 cgaaatgccc cacgtagtcc tctcaacgtc agcccctggc gggagcggta cttccacctg
    27661 accgtagcgc agaacggcat cctgctcgct ggtaagcgta atttcatcga gatacagcgc
    27721 atcgctgtca atctcattgc tggtcaccgg ctgcagagta aagctcatct cttcttcacc
    27781 ggggataagc cgatacaact ggcgttcaac ggtcaccgga atgttacttt gttgagccgt
    27841 ttttgccggt tcacgccagc ggacctgcac attttgcggc gataattcgt caccaaaaga
    27901 gagaatgtcc ggcacaccct gaccaaccca acgccagtct tcgccccctc cagttaactt
    27961 atgtttagcc catgcgcccg caggcgcagg caaaacaact ggaggcattg tcgccatata
    28021 tttcgccagc cagttcatgg ccagcgcgcg ctcaatagtg gattgctcag cggtcaaacc
    28081 acttaaaata gcggccgcct gggtagcgtc accaccgcta ttgagcagca caaccgtgtg
    28141 cgccagcggc tgattgctgt ttgccgcttt atcccacgca tattgctgaa cctgtggcat
    28201 caccgtggac ttcaactgcg cttttttcag caacgtcatg gttaagacgt ttgccaccgc
    28261 atctgccagt ggagattccg gtgtatcgag gatcaggctg tcattgatat cgctggtgtc
    28321 ttcctcagag aaatcttcag ttttagttcc gcgccgggcg atagcttcat ccaacccttt
    28381 caacaacgtt ttgcacggta gattcatctc ctgtgcccac gccagcacca gcgcgcgatg
    28441 caatatcggc atgttatctg cctgctcggc ataactgtcg agcatatgtt gccagtaatc
    28501 cggttgctgc gttacgccga gcgcctggct ggcctgccag tcggcgtacc atgcccatgc
    28561 cgtcaggaag gcgtcgccat tgccatcttc accccaccag gtaaagcgcg ctccgggccc
    28621 cgccagttgc atcagccgca gacggttatc ctgaatcatc tgacgaatgt cgttagcggc
    28681 ggcactttga tgatcggcaa gtgaacgcca ggcgagactg agcgggatca gacggctacc
    28741 ggtgttgatc acgccccccc acggttcatc gactaacgca tcaagattgt tacggaaaat
    28801 ctcctgcggc gtttcgctac tttgtagctg gatattgctc gcctgctcgg gcaacatcag
    28861 cgcgttatcg ccaccgccga gcatgacgtt tttctgctgt tcaacgggcc agctgttatc
    28921 cacaaaagac agttttgtgc taatgctgtc ctgtacttgc ccattttgtt gcagttctgc
    28981 acttaacaag ccagattgct gaatattctg cgtcagcgaa atataattcg cccctttgtg
    29041 cagcgtcagc gtctggcgca tctcagcacc tgcaaattta gtcaccagcg ccaccggttc
    29101 gttatcctgc tgactgaaga taaacagtcc tgctgccggt ttgtcgccca tgcgatacac
    29161 cgttggcata ctccacttca tgtagagatt tttttccgaa cgcagataag cacgcccctg
    29221 cccgaccagc ccgtcgccgt tcatcccacg cgcggtgatg cgccagcggg ttaacgaatc
    29281 aggcatcagg aacgtgaaat acgctttgcc ttgtttatcg gttgtgagtg acggcatcca
    29341 tgccgcggta tccacctctt cacgccgtgg acgttcaagc atttttactc gccgctcgct
    29401 gcggttagtc gcgccaggcg caaccggctc gctggagagc gcctggtcgt agctgataaa
    29461 cgacagactg gagctggtac gcacattgtt acgccccagc ggatagaaaa atttgccgat
    29521 attcggcgcg atttccggtt gcagcgcgta gatcatttca tcgaccacac caaccgttag
    29581 ctgcgcagaa acaggtttac ctttcagcga cgaggttaat tcgacattga ccagttcacc
    29641 aggctggtaa tgggtttttt ccgttttcac ccggatatcc agctggggaa cggcaacttt
    29701 gatcccggcg ttctgaaaac tgtactgacc gttacgggta tacagcaccg aaaaagtgat
    29761 gttaggcgca aaggaattgc tcactggtat gcgggcttca tactgggtat cgtttaaacg
    29821 ttgtaacgtc agccagtttg ccggatgcga tagcagcgac tgctgttcaa cgcgatcgcg
    29881 ttccagcgtc aataatgctt catcaattgg ctccgggaag gtaatcagca tcttcgcggt
    29941 ttcgcctggc tggtacagcg ttttatccgc cacgatatct accgtacccg tatgcgccgt
    30001 gctgccctta ccgctgactg catggcttaa cccggcgaga attaagccgt cttcatcgcg
    30061 taatgtcaga ttgtagttgc caggtttatc gaaattgacg gtaaaggatt tgccgcctga
    30121 cggtagatct ccgctatggc tcgtgcggtc ttcgagacgc aaccattcat acgtaacagg
    30181 aacctgtttt gaagattcca gcgcggcata acggaacaca accgactcgc cgctattact
    30241 gtattgtgcg gcggtactta aagagtaatg cgccaggccg cgttcaatga ggatctcttt
    30301 ggtggtggtg acgcgatacg ccgcgccgtc actggcggag acggttaaca aatagcggct
    30361 cggtttatcg gcggcgggga gatttaaagc cacatgaccg ctatcatcgg acaccgtttc
    30421 gctgccttcc agcgacacgg gaaaacgtcc ggcataacgc aaatcgttac cgaccattga
    30481 taattgctga gcgcgcaaac ttaactgcac gcgggcatct tttaccggct ctccatccgg
    30541 gtaaagcagt tgcagtttgc cgctcaccgc ttcgcctgtt ttgaactctt ttttgtcgag
    30601 agctaaacca atctcgaaat gtggcttgat gtagtttgcc acgcgaaaac tgctgctata
    30661 gacctgtttg cggtaagcca ggcgtaactc ataacctccg gcgacggcat tttctggcag
    30721 gcggaaactc ccctgcccgc cattacgcgc atcaagcgtg acattgacgg tttgcaacag
    30781 actgccgttg gcgtccagca ctgaaagctt cgccggggcg ctgacgatgg gggatgaatg
    30841 caacgggtcg tggaactcgc ggcccatcac tttgacatca acacgatcgc ctgcacgata
    30901 cagagggcgg tcggtaaaga tatacaagcg ggtgttgtag atttcgcttt cgtagaaaaa
    30961 attctcggag acaaaaacgc cgccttcagc atctttaccc agaatgtatg aacgttccgg
    31021 cgatatatgt tgtaactgca aggtaccgct gtcatcggtc acaccgcggg tcatcacgcc
    31081 aagaccgtca gtccacaaga tctctgatcc cggcttcgct tccccctgtt ttttacccgc
    31141 ggtccacacc agaagctcgt tacctgacac tttgctaagc gccacggtat cggaaacaaa
    31201 caccaccgtc gtcgcccgat acccacctac catcgcctcg acgaggtaca gccccggctc
    31261 ttgcttgcct agcggaatat agatgttgcc cggctgcggc gagatgaaat tgctggatgc
    31321 gccttccagt tttacccctt gctgcggctc gaccggttta gcctgccata atgggtaacg
    31381 gaactgttcc accaggggat attttttcag cggtgaaaac tggttgttct gtacataacg
    31441 ggaaggttta ataatggcat taccgagctg taattcgggt aacgcctgag tcacattctg
    31501 ccgagactga gaagaaaaag tacgctgcat cacgcggcga gatttgccgt accagttatc
    31561 ccacagccag gtcagcgtat tgttcagccc gtcgcccaaa tattgcggtt gcaccacaat
    31621 gcaatgcagg tttttctgct ggcgcaaaaa tgccatcggg tcaggaatac gatacaggcg
    31681 aacgtccacg ccgccgtact cttccatctg atagcgtcga taatcacgcc ccggcgcttc
    31741 cagtcgcact ttcgcctctt cactgctgct aaaactgctg tcagcgagca aaaagaatgt
    31801 tcccccggcg ggcggcgcat agttgctgga aggaagcgaa tcatcagcat tggcaagccc
    31861 tgttcccaca agagacagga acaagcacgc tgctatcgcg ccagaaaatt taaacgataa
    31921 atgccaatga aattgggatt ggaatcgttg ggtatccatc gggtgtcctt ccatgtcata
    31981 agttgttgca gactgactgc gcgcattccg ttgtcagttt tcgtggcgct tccggtgtgg
    32041 tagatgacgt aacgccccat ccagaccatt aagtgctggg catcgccctg atcgaaaaaa
    32101 atcatatcgc caggcagcgc ctggtttatg tcctggccaa taaattggct gttgtactga
    32161 atcaaattaa ttgcggtcac gtaggggccg gttttcccgt tcccctgatt ccagttttgc
    32221 gccagttgcc gctgttcagg tgttagcgtc atctctggcg gcaaatactg gttagataac
    32281 ccgttacttt ttagccattt actgtcgtga actttcagcg tctcgttcgc tgcaaatcgc
    32341 accaggcccg cacaatcctg ctgataccag cgtggacttg gaccctggcg gagctgttcc
    32401 tgtgcaatgc gtacaaacca ggcgcgaaac agcccggatt gttcgacatt cagcatttcg
    32461 ctatgggtaa caacacaaca cagccagcaa atcagcgcca gcagcccgtg tctcatagcg
    32521 gttgccaggt tatgggtagc cactgccagg cggcaccggg ttccatctgg gccagcttca
    32581 tgacataacg cggttgttga gataaagcgt ccagcttcgg cattaataaa gtttgtgcgg
    32641 cgttataaaa aaccggttcg agattcttcg gcaaactggt cagcgtttcg ttacgcagca
    32701 gtttcgccac gccttgtgga ttgatataga gcggaacgat gccgtcagtg ggtatcacat
    32761 ccaccattgc cgggcgggtt ttattcagtg tttgcagcgc gttattaacc agcgtgtcat
    32821 cgagggagaa aagcatcgtc ttgttttgca tcgccagcga cacgcggaaa aaataatccg
    32881 acattaattg atcgggttgc accgcctgcg ctttcggata ctggccgtat cgggaactca
    32941 cttcgcgacg ccagatttgc gcttcgccct gctgagtctg gtttaccggc aaaacacctt
    33001 ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa cagtttccca ggcagttgtg
    33061 cctgttcggc tgagccatca aattgaccaa caaatagcgg ggtttgtaat tttgagtctt
    33121 cataccagca cagcccggct gcaccatcta acgccccgtt cagcttgtcg ttttcctggc
    33181 tgatgtgcga aagcatctct tcggcaatac catgcgaata cggcaccgcc acacagaagc
    33241 tggccccggc aggcatactg ttccataccg gcgtaaaatc gaaactggca tctacgctgg
    33301 cggattcatc atttaacgcc acaaagctat gccagccgtc gttgctcatt tcgaagcgta
    33361 cgccagcaaa agaaggcatt aatcgctggt agccaaaccc cagcaacctg gcgctgacaa
    33421 caatacgctg gcgtactggc gttttttcag cagcacgctc ttccaggcca aagctggctt
    33481 gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc cgtggcttcg gtatcctgct
    33541 ggtcatcttt aaacagcata tccgtactgg aaaacaccag catcttgtcc tgataagtcg
    33601 cgaacatcag ggcgttattg ccgttatagc gcaactgata aaccgggatg gtttcactat
    33661 ttagcttaat actactgatt tccgttttgc ttaactggct gtcgctggta gcggcaaaca
    33721 acaatggctc caacagtttg cttaaaccac tgcgctggat cagcaccatg taatgtgaaa
    33781 gatggccttg tttatcgtgc catagcgccg cctgcgcggg ctgatctaag agtgacgaaa
    33841 agagcttatc tttcagcgtg agatcgtgtt catagacaat gcggcgaatg ctgccttcaa
    33901 tgcccagacg atcggcatga ttctgataat agaaaacgaa atcttcactc agaacatcgt
    33961 ggagaaacgg aatggtaagg aggtctttgg gaagctggct cagagagtcg ctgtcgagaa
    34021 agaggtccgg ctcattgaga tcgatttgca gattgttgtg caccaccagc ggcgacaacg
    34081 ttttttctgg cccactgcca gcatattgca acgcccagac gccagcggaa agcagtacta
    34141 ttgcgcccaa acctacaaga ccatagaacc gccagccttt cgcctttttt tcaccactca
    34201 ttgccacatt ccttgtgtaa agccagccat tttttacgag cacagcaaaa ctttaccgcg
    34261 ccctaatacg acaaaagccc agactttgca gcctggactt ttcaattcaa acaagggaga
    34321 tagctccctt ttggcatgaa gaagtaaaat tattcttctt ctggctcgtc gtcaacgtcc
    34381 acttccggag cgatttcatc gtccccttcc gcggcactgc catcgatggt atccagatct
    34441 tcctcgtcaa ccggttcagc aacacgttgc agacccacta cgttttcatc ttccgcagta
    34501 cggatgagga tcacgccctg ggtgttacgg cctacgatgc tgatttccga aacgcgagta
    34561 cgtaccagcg taccggcatc ggtgatcatc atgatctggt cgcagtcatc tacctgcacc
    34621 gcgccaacca ctaaaccgtt acgttcggta accttgatgg agataacccc tttcgtcgca
    34681 cgcgacttag tcgggtattc cgccactgcg gtacgtttac cataaccgtt ctgcgttgcg
    34741 gtgaggattg cgccatcgcc acgaggcacg atcagagaga cgactttatc gccttcgcct
    34801 aaacgaatac cgcgaacacc ggtggtgttg cagcccatcg cacggacaga agactcttta
    34861 aagcgcacca ctttaccttc ggcggagaac agcattactt cgtcttcgcc gctggtcagg
    34921 tcaacgccga tcagctcatc gccttcaacc agtttgatcg ccactttacc ggcggtacgc
    34981 agacggttga actcggtgag gacggttttc ttcacagtac cgttagcggt cgccatgaag
    35041 actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg cagtgatacg ttcgtcctgc
    35101 tccagcggca gcaggttgac gatcggacga ccacgcgcgc cacgagtggc ttctggtaac
    35161 tggtagacct tcatcgaata gacgcggcca cggctggaga agcacagaat atggtcgtgg
    35221 gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt taatacgtgc ggcagattta
    35281 cctttcccgc cacgacgctg cgcttcatat tcagaaagcg gctgatactt cacgtaaccc
    35341 tggtgagaaa gcgtgaccac cacatcttcc tggatgatca gatcttccag gttgatgtcg
    35401 gcgctgttag cggtgatttc agtacgacgt ttgtcaccga actgttcacg aaccagttcc
    35461 agctcttcgc ggatcacttc catcagacga tcggcactgc caagaatacg cagcagttct
    35521 gcgatctgat ccagcagctc tttgtattcg tcgagcagtt tttcatgctc aaggccggtc
    35581 agtttctgca aacgcagatc cagaatcgcc tgagcttgct gttcggtcag gtagtacaga
    35641 ccatcacgca cgccgaactc tggctccagc cattccggac gcgcagcatc atcgcccgca
    35701 cgttcgagca tggcggcaac gttgcccagc tgccacggat tagccaccag cgcagttttc
    35761 gcttctgcag gcgtcggcgc atgacggatc agttcgatga tcgggtcgat gttcgccagc
    35821 gctacggcta atgcttcaag gatatgagca cgatcgcgag ctttacgcag ttcgaaaata
    35881 gtacgacgag tcaccacttc gcggcggtga cgaacaaacg ccgcgatgat gtctttcagg
    35941 ttcatgatct tcggctgacc atggtgcaat gccaccatgt tgataccgaa agaaacctgc
    36001 aactgggtct gggagtagag gttgttgagc acaacttcac cgaccgcatc gcgtttcact
    36061 tcaatcacga tgcgcatacc gtctttgtca gactcgtcac gcagcgcgct gatgccttcc
    36121 acgcgttttt cttttaccag ttccgcaatc ttctcgatca ggcgcgcttt gtttacctga
    36181 tacggaattt cgtggacgat aatggtttca cgtccggttt tggcgtcaac ttccacttca
    36241 gcacgggcgc ggatatatac cttgccgcga ccggtacggt aagcttcttc aataccgcga
    36301 cgaccgttaa tgattgccgc cgtcgggaag tccggcccgg ggatgtgttc catcagccct
    36361 tcaatgctga tgtcttcatc atcgatatac gccagacaac cgttgatgac ttccgtcagg
    36421 ttgtgcggcg ggatgttggt tgccatacct acggcgatac cggaagaacc gttcaccagc
    36481 aggttaggaa ttttggttgg catgacgtcg ggaatttttt ccgtgccgtc atagttatct
    36541 acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt catgggcaat tttcgccaga
    36601 cggatttccg tataacgcat tgccgccgca gagtcgccgt cgatggaacc gaagttaccc
    36661 tgaccgtcta ccagcatgta acgcagcgag aatggctgcg ccatacggac gatcgtgtca
    36721 taaaccgccg agtcaccatg gggatggtat ttaccgatta cgtcaccaac gacacgggca
    36781 gattttttat aggctttgtt ccagtcattg cctagtacgt tcatggcgta aagtacgcga
    36841 cggtgtaccg gcttcaggcc atctcggaca tctggcagcg cacggccaac aatgaccgac
    36901 atcgcataat ccagatagga gctcttcagc tcttcctcaa tgttgaccgg tgtaatttct
    36961 ctcgcaaggt cgctcatcta accgctatcc ctctactgta tcccggattc aaaggtcgca
    37021 aattataaca cagccgcgca gtttgaggta aacctatacg ctttattcac ctccaatgcc
    37081 tgatatactc gtttgtcttg ccaattacgg agtagaagtg ccaatgaatg ccgaaaaatc
    37141 gccggtaaac cataacgtag accacgaaga gatcgctaaa tttgaagctg tcgcctcccg
    37201 ctggtgggat cttgaaggtg agttcaaacc gctgcaccgc attaacccgc tgcgtctggg
    37261 ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag gtgctcgatg tcggttgtgg
    37321 cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg acggtgaccg gtctggatat
    37381 gggctttgag ccattgcagg tggcaaagtt gcacgcgctg gaaagcggca ttcaggtaga
    37441 gtacgtacag gaaaccgtgg aagagcacgc ggcaaaacat gccgggcagt atgatgtagt
    37501 gacctgcatg gagatgctgg agcacgtccc cgatccgcag tcagtggtca gagcctgtgc
    37561 gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg cttaaccgca acggcaagtc
    37621 atggctgatg gcggtggttg gtgctgaata tattttgcgc atggtgccca aaggcacgca
    37681 tgatgtgaag aagtttatta aaccggcaga attgctgggc tgggtggatc agaccagttt
    37741 aaaagagcgg catatgaccg ggctgcatta caacccgatc actaattcct ttaagctcgg
    37801 ccccggcgtg gatgtgaact atatgctgca cacgcagaat aagtgaggtt gttgtttgga
    37861 ttcatccgac aaccgtgcca atgcctgatg cgacgcttgc cgcgtcttat caggcctaca
    37921 aatgctcccg taggccagat aaggcgttta cgccgcatcc ggcaaaagtg ccggtcagac
    37981 agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa atcatcactc
    38041 cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt gcccgtgact
    38101 cccactttta tttcaccccg ttgcttcacc ccatcgtcac tgatagtgct gccatcctct
    38161 tcaatttccg tcgcatgggg atcgtgataa taattcagat ctaacgttgg cgtgacacca
    38221 acagcagtac tccattcgct gtgtaaaccc agccgcgtct gaatatcatc accctgcgat
    38281 tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc ctgataaatc
    38341 acctgcgtct gcggttcaat caccacacca tgccccggta accactgata accggcttcc
    38401 agcgaggcga taatccccga cgagtggtaa tgatcctcgc catcgtcttg ttcggaaaca
    38461 tcattgttaa accacgcata ttgcagccag ctatccagcc agaccccctg cttctgatta
    38521 ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg gttatcggcg
    38581 cgagttccgg tcatattcga gcggctgtcg ccctggttat cgctgtagcc accaacaatc
    38641 ccaagcatcc actcgccatc atcaccccag tgcccgctaa acaggttgcc gctaagctgc
    38701 accgtagaag tgtcttcatg ctgagccaac tgccctgccg ccgtgtaatg ataacgccct
    38761 ccgataacgc gtaaattcag cgtctgacca tcgccaccag cctgatcgcg tcgctccatc
    38821 acaaatgcct gatttgccgc acgtaggtta ttaaaatatc caccaacttt ggcattcaac
    38881 accggctggt aagcaggcgt aggtccaggg tcgggggttg ggtcgggatc cggcgtggga
    38941 tcaggatctg gagtcgggtc cggatcgggc ggcggcgtcg gagtgacttc ttgcgatcgc
    39001 agataccagt cgttgttatc ttccaccagc gtatagtcat acgcccccat attgacgtag
    39061 ccgcttcctg ccagacggaa ctgcgcattg ttttgaaact gcgtgggatc agctgcgaaa
    39121 tcaaccactt taatacctgt agatgtcggc tcaccaatac ccgtaatggg gttaattacc
    39181 acggccgtgt tgccagcagt attaccgttc aataccagtt ggtcacttgc cgagtcatcg
    39241 ccgtttaatt cgctgtcgag cagtagcgta ccgccgccgg tataatcacc atttaccgtc
    39301 agcgtgtcgc cagcgacgcc attttgcagg ctaatcgcac cggacgtatt ggtcagattg
    39361 ccattaaccg tcacgtcgga aagataagtc aggctgtcag tttcaaggtt ggtattaagc
    39421 tcgcctgtca gcgtcacgcc atcggtggca ttcaccaacg taccgtcgcc gctaaacagc
    39481 gaggcatcca gcacctggtt cgtatcctgc tgcgtcaatc gcaacacagt accatcggtt
    39541 atgtcgatat cgccgccaga ggtggcatcg attgagcgaa tatcctgatt ttgcccggtt
    39601 tcaaaaacac cgtcttcccc cacccacagt gaagaaacat aaggaataat attctccgct
    39661 tcacctttta gcgttccgtc ctccacacgt accgcagacg tcgcagtgcc gctggcggtc
    39721 aactccagcg taccgccgcc acttttagtc aacgtgctta cgctctccag ttgttgcccg
    39781 gtactatccg ccatcaaccc gccccattgc gtatctacgc catcctcaac gtgtatttcg
    39841 ccatcagcgc gcatttcaat attcctgccc tgacctgttg tagccgagcg agtgtcaaca
    39901 tcagtcgtca catccatttc actgtgttgt tggggatctg tgaagatcac tgaacggttg
    39961 taacttgtgt cccccaattg cgaattatcg ctgacttcaa gcgtaccgga ggcgatttgc
    40021 gtattaccga gataaccgtt gttattcgcc agcgttacgc gctgatcccc cgcattaatg
    40081 acgacataaa cgccattctg gctggcatca ctgccgccaa tatgtaaatc atcgccactg
    40141 ctgatatcaa cgacatcatt actgccaatc actgtccccc cgctgccgct gatttgcaga
    40201 ccgtcattgt atgagctatc gccatcccag gtggtgaaat cagagagatc caatactccg
    40261 ccatttagca cgatcgattg cggatcgctc tgcattgccc gtaaatcggc ctgattcccc
    40321 gctagcgaca atacggcgtt atcttccacc acaatatcgc cggttaatgc catcgactgc
    40381 gcccctgtca ggagatagct gccatcttgc gctaccgtca actgtccttc accctgaatc
    40441 gagccggaaa aaccgccctg gtttacggta acattgccgc ctgcgtcgat attcaaaatg
    40501 ccattagcaa aaccggttaa tgagtgcaca aacgtttgtt gtgtattgcc aacattcagt
    40561 tctgcctgat actcagaatg aacagtgctg ccgaccatca gaccaaagca atcctgcgga
    40621 tcgctctgac agtgcgtgtc gccgacattc attaactcat cgctgcgccc caacgtcacc
    40681 tcgccattct ggatacttat ctcgccggta aacgcattat tgtcggcatt gaggactaac
    40741 tctcccgctc cctctttaac gatgactccc gttccggcaa gagaatcaat cgcgccatcg
    40801 ttgctggtat tgcctattac cagcgtttta tcggcggcaa tgttaaattc agccacacta
    40861 tgaccgatat acataaagcc ccccgccgct gccgtcgccg aatggtcata tcccgaggcg
    40921 ctgttatttt catcaatcat cacaccattg ttttggtcat aattatcatc aacagaaata
    40981 tcaataagat acggggatgt ggtgctgctg gtataaattg ctccgccata accttctgca
    41041 tagttattgg taaaggaggt attattaatt atcgtttt
//
LOCUS       DAZKJR010000027        39532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKJR010000027 DAZKJR010000000
VERSION     DAZKJR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.027"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(241..507)
                     /locus_tag="ACHOWS_001470"
                     /pseudo
     CDS             complement(241..507)
                     /locus_tag="ACHOWS_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(783..2099)
                     /gene="hipA"
                     /locus_tag="ACHOWS_001471"
     CDS             complement(783..2099)
                     /gene="hipA"
                     /locus_tag="ACHOWS_001471"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFQ0047072.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY"
     gene            complement(2099..2365)
                     /gene="hipB"
                     /locus_tag="ACHOWS_001472"
     CDS             complement(2099..2365)
                     /gene="hipB"
                     /locus_tag="ACHOWS_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFQ0047073.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(2558..4447)
                     /locus_tag="ACHOWS_001473"
                     /pseudo
     CDS             complement(2558..4447)
                     /locus_tag="ACHOWS_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(4597..6189)
                     /gene="lsrK"
                     /locus_tag="ACHOWS_001474"
     CDS             complement(4597..6189)
                     /gene="lsrK"
                     /locus_tag="ACHOWS_001474"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFQ0047074.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDPEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVNELKELHNNTFENKVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLIDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(6268..7221)
                     /gene="lsrR"
                     /locus_tag="ACHOWS_001475"
     CDS             complement(6268..7221)
                     /gene="lsrR"
                     /locus_tag="ACHOWS_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFQ0047075.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            7470..9005
                     /gene="lsrA"
                     /locus_tag="ACHOWS_001476"
     CDS             7470..9005
                     /gene="lsrA"
                     /locus_tag="ACHOWS_001476"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFQ0047076.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLVALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGD
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            8999..10027
                     /gene="lsrC"
                     /locus_tag="ACHOWS_001477"
     CDS             8999..10027
                     /gene="lsrC"
                     /locus_tag="ACHOWS_001477"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFQ0047077.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTVLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            10027..11019
                     /gene="lsrD"
                     /locus_tag="ACHOWS_001478"
     CDS             10027..11019
                     /gene="lsrD"
                     /locus_tag="ACHOWS_001478"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFQ0047078.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            11031..12053
                     /gene="lsrB"
                     /locus_tag="ACHOWS_001479"
     CDS             11031..12053
                     /gene="lsrB"
                     /locus_tag="ACHOWS_001479"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFQ0047079.1"
                     /translation="MTLHRFKKIALLSALGIAVISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            12080..12955
                     /gene="lsrF"
                     /locus_tag="ACHOWS_001480"
     CDS             12080..12955
                     /gene="lsrF"
                     /locus_tag="ACHOWS_001480"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFQ0047080.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGIPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            12979..13269
                     /gene="lsrG"
                     /locus_tag="ACHOWS_001481"
     CDS             12979..13269
                     /gene="lsrG"
                     /locus_tag="ACHOWS_001481"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFQ0047081.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            13326..14084
                     /gene="tam"
                     /locus_tag="ACHOWS_001482"
     CDS             13326..14084
                     /gene="tam"
                     /locus_tag="ACHOWS_001482"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFQ0047082.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STTLLHQRWPAARITGIDSSPAMIAEARSTLPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQSIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(14088..15002)
                     /gene="yneE"
                     /locus_tag="ACHOWS_001483"
     CDS             complement(14088..15002)
                     /gene="yneE"
                     /locus_tag="ACHOWS_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFQ0047083.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISVVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(15199..16650)
                     /gene="uxaB"
                     /locus_tag="ACHOWS_001484"
     CDS             complement(15199..16650)
                     /gene="uxaB"
                     /locus_tag="ACHOWS_001484"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0047084.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTGLVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILKKGMREAVRPLC"
     gene            complement(16878..18296)
                     /locus_tag="ACHOWS_001485"
     CDS             complement(16878..18296)
                     /locus_tag="ACHOWS_001485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFQ0047085.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(18435..18794)
                     /gene="yneG"
                     /locus_tag="ACHOWS_001486"
     CDS             complement(18435..18794)
                     /gene="yneG"
                     /locus_tag="ACHOWS_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFQ0047086.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(18794..19720)
                     /gene="glsB"
                     /locus_tag="ACHOWS_001487"
     CDS             complement(18794..19720)
                     /gene="glsB"
                     /locus_tag="ACHOWS_001487"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFQ0047087.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRMGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCIELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(19784..21172)
                     /gene="sad"
                     /locus_tag="ACHOWS_001488"
     CDS             complement(19784..21172)
                     /gene="sad"
                     /locus_tag="ACHOWS_001488"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0047088.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNNGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            21273..22148
                     /gene="yneJ"
                     /locus_tag="ACHOWS_001489"
     CDS             21273..22148
                     /gene="yneJ"
                     /locus_tag="ACHOWS_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0047089.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAATKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPAMLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            22232..22351
                     /gene="yneK"
                     /locus_tag="ACHOWS_001490"
     CDS             22232..22351
                     /gene="yneK"
                     /locus_tag="ACHOWS_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFQ0047090.1"
                     /translation="MVTPVSISNFISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            22493..23683
                     /gene="ydeA"
                     /locus_tag="ACHOWS_001491"
     CDS             22493..23683
                     /gene="ydeA"
                     /locus_tag="ACHOWS_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFQ0047091.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGELITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(23708..24373)
                     /gene="marC"
                     /locus_tag="ACHOWS_001492"
     CDS             complement(23708..24373)
                     /gene="marC"
                     /locus_tag="ACHOWS_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFQ0047092.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            24585..25019
                     /gene="marR"
                     /locus_tag="ACHOWS_001493"
     CDS             24585..25019
                     /gene="marR"
                     /locus_tag="ACHOWS_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFQ0047093.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            25040..25423
                     /gene="marA"
                     /locus_tag="ACHOWS_001494"
     CDS             25040..25423
                     /gene="marA"
                     /locus_tag="ACHOWS_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFQ0047094.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            25455..25673
                     /gene="marB"
                     /locus_tag="ACHOWS_001495"
     CDS             25455..25673
                     /gene="marB"
                     /locus_tag="ACHOWS_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFQ0047095.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(25704..26603)
                     /gene="eamA"
                     /locus_tag="ACHOWS_001496"
     CDS             complement(25704..26603)
                     /gene="eamA"
                     /locus_tag="ACHOWS_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFQ0047096.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            26798..27985
                     /gene="ydeE"
                     /locus_tag="ACHOWS_001497"
     CDS             26798..27985
                     /gene="ydeE"
                     /locus_tag="ACHOWS_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFQ0047097.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRNEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            28112..28207
                     /gene="mgtS"
                     /locus_tag="ACHOWS_001498"
     CDS             28112..28207
                     /gene="mgtS"
                     /locus_tag="ACHOWS_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFQ0047098.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            28207..28311
                     /gene="mgtT"
                     /locus_tag="ACHOWS_001499"
     CDS             28207..28311
                     /gene="mgtT"
                     /locus_tag="ACHOWS_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFQ0047099.1"
                     /translation="MNGDNPSPNRPLVTTVYKGPDFYDGEKKPPVNRR"
     gene            complement(28426..29316)
                     /gene="dgcZ"
                     /locus_tag="ACHOWS_001500"
     CDS             complement(28426..29316)
                     /gene="dgcZ"
                     /locus_tag="ACHOWS_001500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFQ0047100.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYELCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(29571..29963)
                     /gene="ydeI"
                     /locus_tag="ACHOWS_001501"
     CDS             complement(29571..29963)
                     /gene="ydeI"
                     /locus_tag="ACHOWS_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFQ0047101.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            30203..30283
                     /locus_tag="ACHOWS_001502"
                     /pseudo
     CDS             30203..30283
                     /locus_tag="ACHOWS_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30328..32373)
                     /gene="dcp"
                     /locus_tag="ACHOWS_001503"
     CDS             complement(30328..32373)
                     /gene="dcp"
                     /locus_tag="ACHOWS_001503"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFQ0047102.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELVSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KSAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            32510..33256
                     /gene="ydfG"
                     /locus_tag="ACHOWS_001504"
     CDS             32510..33256
                     /gene="ydfG"
                     /locus_tag="ACHOWS_001504"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFQ0047103.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            33345..34031
                     /gene="rspR"
                     /locus_tag="ACHOWS_001505"
                     /gene_synonym="ydfH"
     CDS             33345..34031
                     /gene="rspR"
                     /locus_tag="ACHOWS_001505"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFQ0047104.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTHHLQEISESVRQIR
                     QENSDWFSEE"
     gene            34208..34411
                     /gene="ydfZ"
                     /locus_tag="ACHOWS_001506"
     CDS             34208..34411
                     /gene="ydfZ"
                     /locus_tag="ACHOWS_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFQ0047105.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(34447..35907)
                     /gene="ydfI"
                     /locus_tag="ACHOWS_001507"
     CDS             complement(34447..35907)
                     /gene="ydfI"
                     /locus_tag="ACHOWS_001507"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0047106.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAVMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(35997..37364)
                     /gene="ydfJ"
                     /locus_tag="ACHOWS_001508"
     CDS             complement(35997..37364)
                     /gene="ydfJ"
                     /locus_tag="ACHOWS_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFQ0047107.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVTAAQ
                     PSRSL"
     gene            complement(37422..38441)
                     /gene="rspB"
                     /locus_tag="ACHOWS_001509"
     CDS             complement(37422..38441)
                     /gene="rspB"
                     /locus_tag="ACHOWS_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFQ0047108.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(38453..>39532)
                     /gene="rspA"
                     /locus_tag="ACHOWS_001510"
     CDS             complement(38453..>39532)
                     /gene="rspA"
                     /locus_tag="ACHOWS_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFQ0047109.1"
                     /translation="YLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAV
                     DMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIR
                     VQCGIPGMKTTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGF
                     NEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVG
                     EVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSP
                     VCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEK
                     LAAKYPYEPAYLPVARLEDGTLWNW"
BASE COUNT        10041 a        10058 c         9358 g        10075 t
ORIGIN      
        1 gcagcacttg ttgtagagag tatttgcaaa gacattttat gtcgctaaat aacatgacga
       61 tttcattatg catgttattt ataaaccgtg actaaacaga atattattgc gaacaaggga
      121 agtgtcagga aaggtcaaga gcgaccaatg gtttatatgg ctataaatca ttagccatag
      181 ttaatgtttg cctggtgtaa ctaacacgga attgctctaa acagataagt gtagggttta
      241 ttaaagctga ccgacagact ttcttaacct ggtaatatct ctcttaggtg actctccaaa
      301 catccgcgaa tattcccgac tgaaatggga taagctttcg taaccgacag cataggctgc
      361 agtggtgaca tcgtaatgtt cattcagcat cagtcgcctg gcttcattga ggcttagcca
      421 tttccgatac cggagtggac tcattcccgc aagctgtctg aaatgttgat ggaaagaggg
      481 ggtgttcatt tgcacgcggt ttgccagcct gactcttcaa cgctgagaag atgcggcaaa
      541 cggcaaacct gcataccagc cagcctatat ccggtatgca caggccaatt atgaaagagg
      601 agaggcttta tgttctggaa ccttgaagta ataacgtcga ccggaaaatc actctgcatc
      661 atcactcaga ttcaatttaa cataatatac attatgcgca ccaacataaa ccaagggaga
      721 atccagccgt tggcggtcat gattgtcctg ctcattaaca acggccacac cccatatctt
      781 acttaatacc attctcggct taaccgacgg tgcaacctca acacattggt ttcaactgcc
      841 gtcaccacgt tctccggaaa atctgtcggt aatgaagtct tcacgttatc cagtgctgcc
      901 ggaatcattc tggcaaagtc actcaggatt tcatgcatct gcacttgcgg aaatttcagc
      961 acctttgctg tcgccagaaa atgtcgcgga taaattttat cgattgccgt ttttttgcct
     1021 ttggatgcgt taagccccat tgccagtttg agatcgctga tgtgtattcc tgcaccaccc
     1081 aataccggaa atgctgaaat gatgtcgtaa aatggcgtga gtcgataact gccgccagcc
     1141 tgaataaata cggagaagtt ttttgcatgg ccatccgttg cgccaatcaa ccactggaac
     1201 acctgaaact tcataaaatc atagcgatct ttcagcgcct cgctggaccc catcaaaaaa
     1261 gccatgatcc gcgcgatgcc tgggcctcca tctgattcat atttcaccga tgaaggtaaa
     1321 ccgaatgtct gacacatatc ctcctgtggc aagcgaagta aaaccgttcg ctcagcattc
     1381 caacgcctgt caaaacgttc gaccgctaac gcgcgcacat ttcccgcttt aatgatttct
     1441 gcgtccggca cattcaaccc aagttcttta gccagcagca gacagtaata ctcattatca
     1501 acgctttggc tgagatcgag cgtcgcattg ggctgcctga tttcgccaat cggtaattta
     1561 atgatgtgcg tcgtcggcgt tattcctttc ggaatgcacc agtcattgcc tattctgagc
     1621 agcgctgtct tctcctgcgc gccagcaacc gagatgcgaa agtcattttc ttctctaatc
     1681 atgcccagag ggatatctgc tttataagcc gttaatactt cttcaagcct ggcttcagtg
     1741 agctgttccc atgccattat cgggcgcgtt atggtttcat tttcgggtaa taacgttacg
     1801 gcaccaacgc tgtctcgccc tatttctgac aataaatcaa acggttgtct ggatttagca
     1861 tgataacgtt taacgatccg atcacgtaca atcgggctat cgggtaacag gttatcgaag
     1921 aagttaaata cggcatcaga ggtgatattc cccctctgca atggcagcga aagtgacaac
     1981 ggtctggcat aacggctggc taaccactcc ggtgcatact taaaggtgtg cgcgccattg
     2041 gctaacttcg ttaactcgcc tacccgctgg ttgttcatcc aagtgacaag tttaggcatt
     2101 accactccag atcttgctgt tctgttgatt ctggcgaggc atttttcgtg tcgcatagcg
     2161 tcattgagag ttcaagcgac tgtaaaatct taaaaaaggt cgtcagcgtg gtattgtcag
     2221 ggttgttttc gaaattggaa atcgtcgcct gcttaatacc aatttttttc gccagctcgc
     2281 tctgcgtcca gccattttgc tggcgaacca gtttcattgc attcgccaat tgcgttgggc
     2341 tatagatctt ctgaaagctc atcatgtcat acgtccacag caaatttatc cgcttaaggg
     2401 gatattatca gttttatcct ttagtgagga taagtcaatc tggaaacaca tcttatccgc
     2461 tctacgggat aaacactgtg atatccgcga tcgcggataa aaaggaggat ttaaaagtcg
     2521 tttgaagtcg gcaatattaa gccgcatgca tcttcgacat gcggctattc ccttgggtat
     2581 cgccttttac gttcatctta tcggtgggcg aattatcgcc gcccagcgtc gcgttgaacg
     2641 tcatcaggcc attgtttccg gtatagttac cgttaacggt cagtacattc cccggtttcc
     2701 ccccttcgct gttcatttga accgtgccac cattctccag gttaccgccg acagtggttt
     2761 tagcgacacg cagtgtcccc cctggcataa cgttcacatc acctgcagtg gtgacatttc
     2821 ccgacatcac accgctgtta tggatattaa tgtgctgact tgccatatta atggcagagt
     2881 cggaaccgaa agcaatttca ccacccacga tatccgtcgt acccttatac gccgcggaat
     2941 ctttgttaag cgtcaggatc ccggtaccca gttttgtcac atcaccgcta ccgctgatac
     3001 tgtgcgtcat cacccagcta cgctcagtgg agatggtgag caggccatta ttgataacgg
     3061 aagcatcacc caggtttttc tgctcgttga cgctaagcgc gctgcctgta tcaatgttga
     3121 attgccccac aaagcggcta ttatcgccat ccagctctac gtctgtcctg gcggttgcgc
     3181 tcacgatacc cttcccgctt atgctattac gcagttcgcc ggtcacattt tttagcgtta
     3241 acagaccgtc attggcaata tttcctctgc ctaacccctg cgtattatcg aggctgactt
     3301 ccgcatttgg gctaatgctg gttaacgcat tgtagcgtgc cttaagcccc tcgatagcca
     3361 gtgttccccc tgtaacattc aggtttccgc cacctgtcag ttcgccctga ctgatcccac
     3421 ctttattcac cgtcagcgac ccctctttaa acaaaacagt cgaacccatt tgcccggtga
     3481 atgtctctac cgtctgggtc gatccattaa gatcgacgat ggccgcgttg ctgatgttca
     3541 gttcccgggt gttgcccagc gcgccatccg catcggtacg taatgtcccc atctgaacgt
     3601 aggttgcccc ctgatagtcg ttctgaccgt tggaaagcga aacctgtcgt accgtattga
     3661 ttgccagatc cccttcgcca ccgatttttg ccgacatatc gaccgttgcg ccataagctc
     3721 cgccatgttc ggctaacgtc agcttttgcc caccatggat gttcagcgct ttcagcccat
     3781 agttaacata caaaccatca ccaggtgcgg tcgtgaggcg gaatccataa tcaccctcgg
     3841 cgaccaccgt tccgttttgc gcaatatgta acgttttgtc cgcttccacc tcgtcgccct
     3901 gtaaatcacg taacgttaat gaaccacccg atccaataac cgtttgcgcc ttcaccagtt
     3961 gaacgccgac atggctatcg tcgtgttcca tcaaattgag cgtcgtcaga gggttattcg
     4021 ccatgggatc attccacggt ttaggcacgt cgataagcac gtcgcccatc ccgtttacct
     4081 gatagttacg gcctttccag gtgtagtcac ccgcgccgac aaccagcgta tcgacgctga
     4141 tatatccctc cgcaagcgtc gcagcaggta tatccgtatc gaacgtgagc gtaccgccat
     4201 tcatggctag tccaccaatg gattgctctc ccacttttac cgatgtggta ttttcactgt
     4261 cagactgcag catcgcgtaa gtaagcgcag cggtgttgtc gcgttccagt gcgaaggtac
     4321 tggctttcag ttgggcaaca ccggcgaatt cagtccctgc tgcatgggta aagccaaaca
     4381 ttttgtcgga ggatgacagc tgcactcgca tcaagccatc gcctttgagc gcattgatca
     4441 gcgtgaatag caatttcccc ttgaatatag tactggtgat ggaacgatga atctgcaagc
     4501 tggcatttaa caagccagct ctaaaagaag ggaaatgaga ataccaatac tcaaaaacta
     4561 acagccacgg tcatcatggt gtggctgtca atgaaactat aacccaggcg ctttccataa
     4621 cgacgtcgtc agtccatgat caaccagccc cagctgatcc tgataaactg cctgccactt
     4681 atcgcgtgaa tcctgataaa gttcatgctt ttccgggtct ggtgtgtgcg tccgttccca
     4741 acgaaccaag cgttctccgg tttctgccat tgatgaaaaa attccggcac cgacgccagc
     4801 tgcaatggca catcctaatg cagtggcttc tttgaccacc ggaatattga cgggtaatcc
     4861 cgagacatca gcgagaattt gactccataa tttccctttt gaacctccgc cagcaaaaac
     4921 cagcgaggtg ggatgaatat tcgagaaatc agcaatttgc tgtaagttac acgctgatac
     4981 aatcgccgca ttttcttcca gcgcacggaa caatgtcgct ttgttacatt tatccgggtc
     5041 aatggacaag ttaataaagg aaggcgcagc gtgataccag gttttaaagc gcattctgtc
     5101 ggagaatatc ggcattacgc cccacgaccc aggcggcacc cgactggcca tctcttccag
     5161 cagcgtatag gtgtcgatgc ctaaacgttc ggcaatcagt ttttcttcgg cacagaaagc
     5221 atcgcggaac cagcgcatgg tgagtccggt aaaaaagctt atagattcag cttgtaccat
     5281 gccaggaata acatggggat taacgcgcac gttcatttct gggtctgtca ccggcgcggc
     5341 taagtttaca acttgctgcc agaatgtgcc gccaagaacc gcggtttgtg ccggacgtac
     5401 aacgcctaac cccaggcaac caagctgcac gtcacctcct ccaacgacca ccggagtgcc
     5461 cgccttcaga ccgcagagtt ccgccgcttg tgaacttacc acacccagca atgtgccggt
     5521 ttctttgaca ggagaaagaa tatcggcacg caggccagcc atatccagca atgcaggttt
     5581 ccagtcacgg gtggttagat caataagtcc cgtggtgcca gcgttagagg gatccaccgc
     5641 aagttcgccg ctgagcatat aggccagcca gtcgctgatc atggtgatgg ttgatgcctg
     5701 acggtaaata tcggaacgat ggtgcgccag ccaaagtaat ctggggatgg cgcttaaagc
     5761 cagcgtttgc ccggtcgctc gataaacttt gttttcaaag gtattgttgt gcagttcttt
     5821 aagttcgtta acttcgcgcg ccgctctggc atccacattg gcgcaggccc agatcggtgt
     5881 tccttcattg ttatataaaa caatgccttc acgcatcgaa catgccgaaa cagcagcgat
     5941 atactccggg gctatgccgg cgttgtgcag cgcctggcgc atacactcac acgccagttg
     6001 ccagtttttg ttgagatcaa attccataga accaggaacg tccggtactg ccagatgccg
     6061 ccactccgcc tgtcccacgg ctatttgatt gccttccggg tcgaatatca cagcccgaat
     6121 acttccggtg cctgcatcca gcgccatcag gtagtacttt gattctgaag gggtaaagag
     6181 tcgagccatt agattatcct cggcttatgc gttctgtctg ttcctctata cgttctccat
     6241 cattcccggt aacaaggtca tgcaaattta actacgtaaa atcgccgctg ctgtgtcctg
     6301 atcggtaacc agtgcgttga tatagccgcc tttcattgca gcggcaatgg cttcggcttt
     6361 attttctccc cctgccacgc caacccggac gggtatggtc ttcagcgcgc ttaaaggtaa
     6421 gccaatcagt tcgttatgta ttttaatatc cgtgacaacg tcaccttttg catcaaaaaa
     6481 gtagcctaaa atgtcgccaa cagccccttt tcgaccaatc attaactgtt cgccctggct
     6541 gatatagccg gagcgaatga ttgttgcgtc gtcttgttga ctcacagccc caatgccgac
     6601 aatcgccaca tccgctgctt gcgcggctag cagaacatct ttgacgcaat tttcattttt
     6661 tagcgtacgg gcaatgtcag cggaggatgc ccgcaacgga gccggaataa tattcacact
     6721 gcacgccgcg ttaagctgcc cgatacccgt catataagaa ccgactccac cggagagcgt
     6781 gaccaggcga atttgctgtg acgaaataaa accacttaag cgttgcagcg tattcatagt
     6841 tgcctcgcca aaaccaatcg ccagcatctg ttgtggttga agtaaactca tcaacatatg
     6901 cgccgcgcct atccctagtc gcccgccgac atcagcatcc gcaagccctg ggatcacccg
     6961 gacatgttgc agcgaaaact gacgacgtaa ttgtgtttca tattccagac agccttcaaa
     7021 gcgagaatta atctgtacgc gaataatgcc ggattgatgc cctttctcca gtaatcgcga
     7081 cactttcaaa cgggtcaggc cgagacgatc gctgatctca ctctgggtca gcccgtcgtg
     7141 gtagtaaaac cacgcaatcc gcgcgacctg ttcttcttca cacattccct gttctgaaat
     7201 taccgaatcg ttgattgtca taattcattc ttcactttga acatatttaa atctttaatg
     7261 caattgttca gttcttgcgc atttatatct gtgatagcaa ccacagtttg actctacgag
     7321 catgaacaaa cgcaaccgtg aaaatcaaaa tagcataaat tgtgatctat tcgtcggaaa
     7381 tatgtgcaat gtccacctaa ggttatgaac aaattaaaag cagaaataca tttgttcaaa
     7441 actcacctgc aaaactgaac gggggaaata tgcaaacgag tgatacccgc gcgttaccgc
     7501 tactttgcgc ccgctcggtt tataaacagt attcaggggt taatgtcctg aaaggcatcg
     7561 attttacgtt gcatcagggg gaggtccacg ccctgctcgg cggcaatggt gccggtaaat
     7621 cgacgttaat gaagattatt gccggtatta cccctgctga tagcggtacg ctggagattg
     7681 ggggcaataa ttacaccaga ttaacgccag ttcatgctca tcagctgggc atttatctcg
     7741 ttccccagga accgctgctt tttccaagcc tgtcgataaa agaaaacatc ctgtttggtc
     7801 tggcaaaaaa acaactctcc atacagaaaa tgaataactt gctggtggcg ctgggctgcc
     7861 agtttgatct gcatagtctg gcaggatcgc tggatgtcgc cgatcgccaa atggtggaaa
     7921 tcctccgcgg cctgatgcgc gactcgcgga ttctgatcct cgatgaacct acagcctcgc
     7981 ttacccctgc ggaaaccgaa cgtttgtttc gtcgcttgca agagctgctt gctactggcg
     8041 tgggtattgt ttttatctcg cataagctgc cggaaattcg ccagattgcc gatcgaatta
     8101 gcgtgatgcg cgacggaacc atcgccttaa gcggcaaaac cagcgaactg tctaccgacg
     8161 acattattca ggccatcacg ccagcgctac gggaaaaatc gctctctgcc agccaaaaat
     8221 tatggctgga attacctggc aaccgcccac aacatgccgc gggaacgccg gtgctgacac
     8281 tggaaaatct gaccggcgaa ggtttcagga atgtcagcct gacgctcaat gccggagaaa
     8341 ttctgggctt ggctgggctg gtgggagccg gacgcacaga actggccgag acgctctatg
     8401 gtctgcgtac tttgcgtggc ggacgcatta tgctgaatgg taaagagatc aatagattat
     8461 ccaccggaga tcgtttactg cgcggtctgg tttatctgcc ggaagatcgc cagtcatccg
     8521 gacttaatct cgatgcttca ctggcgtgga acgtctgcgc ccttactcat aaccttcgtg
     8581 gattctgggc gaaaaccgcg aaagataatg ccaccctgga acgatatcgc cgggcgctga
     8641 atattaaatt taaccaaccg gaacaagctg cgcggacctt atccggcggc aaccagcaaa
     8701 aaatcctgat tgccaaatgt ctggaagctt cgccgcaagt tttgattgtc gatgagccga
     8761 cgcgcggcgt ggatgtctcg gcgcgtaatg atatctacca actgttgcgc agcatcgccg
     8821 cacaaaatgt ggctgtgctg cttatctcct ctgatctgga agagatcgaa ctgatggcag
     8881 atcgcgtgta tgtgatgcat cagggcgaaa ttgcccactc tgcactgacc ggacgcgata
     8941 ttaatgtcga gaccattatg cgcgttgcct tcggagatag tcagcgtcag gaggcgtcat
     9001 gctgaaattt attcagaaca accgtgaaat cacggcactg ctggcggtgg tgctgctgtt
     9061 tgtattaccc ggttttctcg accgtcagta tttaagtgtg caaacgctga ccatggttta
     9121 tagcagcgcg caaatcctga tcctgctggc aatgggcgcg acgctggtaa tgcttacgcg
     9181 caatattgat gtttcagtgg gttcgattac cggaatgtgc gcggtgctgt tggggatgtt
     9241 actgaacgcg gggtattcac tacctgttgc ttgtgtcgcg actttactgc ttggtttgct
     9301 cgcgggattt ttcaacggtg tcctggtcgc gtggctaaag atccctgcca ttgttgccac
     9361 ccttggcacg ttagggttgt acagaggcat catgttgctg tggactggcg gtaaatggat
     9421 tgagggatta cccgccgaac tgaaacaact ctccgccccg ctgctgtttg gcgtttccgc
     9481 gattggttgg ttgacgataa ttctggtggc atttatggcc tggctgctgg caaagacggc
     9541 gtttggacgc agtttttatg tcacgggcga taatttacag ggcgctcgtc aactgggcgt
     9601 tcgtacggaa gccattcgca ttgtggcatt ttcgttgaac ggctgcatgg cggcactggc
     9661 gggaattgtg tttgcttcgc agattggttt tatccccaac cagaccggta ccgtgctgga
     9721 gatgaaagca attgcagcct gcgtgctggg cggcattagt ttgctcggcg gttccggcgc
     9781 gatcatcggt gcggtactcg gcgcatggtt cctgacgcag atcgatagtg tactggtgct
     9841 gttgcgcatt ccggcatggt ggaatgattt tatcgcgggc atggttctgc tggcggtgct
     9901 ggtgtttgat ggacgcctgc gttgtgcgct ggaacgtaat ctgcggcggc aaaaatatgc
     9961 ccgctttatg atgccaccgc catccgttaa acccgcttcg tcaggtaaaa aacgggaggc
    10021 cgcgtaatgc gtattcgcta cggttgggaa ctggctcttg ccgcactgct cgttattgag
    10081 attgttgcat ttggtgcaat taacccgcgg atgttagatc tcaatatgtt gctgttcagc
    10141 accagtgact ttatctgcat tggcattgtc gccctaccgc tgacgatggt gattgtcagt
    10201 ggcgggatcg atatttcgtt tggttcgacc atcggcctct gcgccattgc attgggcgta
    10261 ctgtttcaaa gtggtgtgcc gatgccgctg gcgattcttc tgaccttact gctcggcgca
    10321 ttgtgcgggc tgatcaacgc cggattaatt atctatacca aagttaaccc gctggtgatt
    10381 acgcttggca cgctgtatct gtttgccgga agcgccctgc tgctttccgg gatggccgga
    10441 gcgacggggt acgaaggtat tggtggattc ccgatggcgt ttacagattt cgctaacctg
    10501 gatgtgctgg ggctccccgt tccgctgatt atcttcctga tatgtctcct cgttttctgg
    10561 ctctggctgc ataaaaccca tgccggacgt aacgtgtttt taattgggca aagcccgcgt
    10621 gtggcggttt atagcgcgat tccagttaac cgcaccttgt gtgcgctcta tgccatgacg
    10681 gggctggcgt ctgcggtcgc cgctgtgctg ctggtatcgt attttggttc agcacgttcc
    10741 gatctcggtg cgtcgtttct gatgcccgcc atcaccgccg tagtgctcgg cggtgccaat
    10801 atttatggtg gttctggttc cattatcggc accgccatag cggttttatt agtgggatat
    10861 ttgcaacaag gtttgcaaat ggcaggagtg ccaaatcagg tgtccagcgc cctttccggt
    10921 gcgctactta tcgtcgttgt cgtaggtcgt tccgttagcc tgcatcgcca gcaaattaaa
    10981 gagtggctgg cgcgtcgggc caataaccca ttgccataaa ggatatcttc atgacacttc
    11041 atcgctttaa gaaaatcgcc ttacttagcg cgcttggcat tgccgtaatc tctatgaatg
    11101 tgcaggcggc agagcgtatt gcatttattc ccaaactggt tggcgtggga ttttttacca
    11161 gcggtggcaa cggcgcacaa caagcgggta aagagttggg cgttgatgtg acctacgacg
    11221 ggccgacaga acccagtgtt tctggtcagg tacagttgat taataacttc gtcaatcagg
    11281 ggtataacgc cattatcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac
    11341 gcgccatgca acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc
    11401 gctcttacta cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg
    11461 cggcgcgtca ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg
    11521 ttacggacca aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag
    11581 gctgggaaat tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg
    11641 cagaaggaat attaaaagcc tatagcgatc tcgacgccat tatcgccccc gatgccaatg
    11701 ccctgcccgc tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagtt gcgattgtcg
    11761 gattcagtac gccaaacgtg atgcgtccat atgtggaacg cggcacggtg aaagaatttg
    11821 gcctgtggga tgtggttcag caaggcaaaa tatcagttta tgtcgcggat gcattattga
    11881 aaaaaggatc aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggtggaag
    11941 tctcgccaaa cagcgttcag ggctatgact acgaagctga tggtaatggc atcgtactgt
    12001 taccggagcg cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat
    12061 tacttaaccg gagtacgtta tggcagattt agatgatatt aaagatggta aagattttcg
    12121 taccgatcaa ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cactggattg
    12181 gggaatgcag tcacgcttat cgcggatatt taatccgaaa acgggtaaaa ccgtgatgct
    12241 ggcctttgat catggttatt ttcaggggcc gactaccgga cttgaacgca ttgatataaa
    12301 tatcgccccg ctgtttgaac atgccgatgt attaatgtgt acgcgcggca ttttgcgcag
    12361 cgtagtcccc ccggcgacca ataagccggt ggtactgcgg gcgtcaggtg cgaactctat
    12421 tctggcggaa ttaagtaatg aagccgtggc gttatcgatg gatgatgccg tgcgcctgaa
    12481 cagttgcgcg gtggcggcgc aggtttatat cggcagcgaa tatgaacatc agtcgatcaa
    12541 aaatattatt cagctggtcg atgccggaat gaaagcggga ataccgacca tggccgtgac
    12601 tggtgtgggc aaagatatgg tgcgcgatca gcgttatttc tcgctcgcga ctcgaatcgc
    12661 cgctgaaatg ggggcacaga ttatcaaaac ttattatgtc gataaaggtt ttgaacggat
    12721 tgttgccgga tgtccggtac ccattgttat tgctggcggt aaaaagttac cggagcgcga
    12781 ggcgcttgaa atgtgctggc aggctatcga tcagggcgct tctggtgtgg atatggggcg
    12841 taatattttc cagtctgacc atccggtggc gatgatgaaa gccgtacagg cggtggtaca
    12901 ccataacgaa acggctgatc gggcatatga actctatctg agtgaaaaac agtaactgcg
    12961 gatttaagga gaagaattat gcacgtcaca ctggttgaaa ttaacgttca tgaagataag
    13021 gttgacgagt ttatcgaagt ttttcgccag aaccacctgg gctctgtaca ggaagaaggc
    13081 aatttgcgct tcgatgtctt acaggacccg gaagtgaatt cgcgctttta tatctacgaa
    13141 gcctataaag atgaagacgc agtggcattc cataaaacca cgccccacta caaaacctgt
    13201 gtcgcgaaac tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt caatgggttg
    13261 atgccgtgag gcgaatttat cagttttatc tacaattggg gtaacgcgct gacgggagta
    13321 aataaatgtc tgactggaac ccctctttat atctgcactt tgccgctgaa cgatcgcgtc
    13381 cggcggtgga gctgcttgcc agagtgccgc tggagaatat caaatacgtt gccgatcttg
    13441 gctgtggccc aggtaacagc accaccctgc tgcatcaacg ttggcctgcg gccaggataa
    13501 caggcatcga ctcgtctccg gcgatgattg ctgaagcgcg cagtactttg ccagactgcc
    13561 agttcgtgga agcagacatc cgcaactggc aaccggaaca ggcactcgat ctgatttttg
    13621 ctaatgcctc actgcaatgg ctgcccgacc actacgaatt gttccctcat ctggtttctt
    13681 tacttaattc gcatggtgtg ttggcagtac agatgccaga taactggcta gagccaaccc
    13741 atgtgctcat gcgcgaagtc gcctgggaac aaaattatcc ggatcgcggg cgtgagccgc
    13801 tggctggcgt tcatgcttac tacgatattc tgagtgaagc cggatgtgag gtcgatatct
    13861 ggcgaaccac ttactatcac cagatgccgt cgcaccagtc gattatcgat tgggtgaccg
    13921 ccactggatt acgtccgtgg ttacaggatc tgaccgagag cgaacagcag cattttctta
    13981 cgcgctacca tcagatgctg gaagagcaat atcccctcca ggagaacgga cagatattgc
    14041 tggcatttcc gcgtctgttt attgttgccc gccgtacgga gtaattatca cgtcagctgg
    14101 taatggcgat cgggaagaat tttcgctgga atttctgctt catcgttcat ctgtaacagg
    14161 tcaatttcaa tagcgttgca gatggcatcc agcggtaagt cattgttttc agtaccgaag
    14221 ggatcttcca gttcttccgc cagacaatcc agcgaaataa aagtgtagga aatcagcaca
    14281 gagataaaag gcgtcatgta atgcaggtcc acgaccagcg caaacggcag catgatacaa
    14341 aacagataca cggtgcgatg caaaatcagg gtgtaggcaa agggaattgg cgtataggca
    14401 atgcgctcgc atcctgccag gaccactgaa atatcattaa gccgatcgtt gaggctaata
    14461 aacaggatat ctgaaagttg tccattgcgt cgctgaaccg ccaaccactc ccccattatt
    14521 aacaagatgc ggttagctgg agagttagaa gccagtacac gctgaagatc ttcagtcttg
    14581 agataatgag ccagcacttc cgcctgtggc tgtttgcgta atgtcatgcg taaacagtgg
    14641 gcgaaggcga tttgcagccg ggcaaactcc cttacacttg ccgaatccgg caatgtcgtt
    14701 tttacctcgc gcagtaacga ccgcgaggca atcatcaact gcccccaaag ttttcgcgct
    14761 tcaacgtaac gagcgtaacc ggcattatta cgaaaaccaa gaaaaatggc gatggcgaca
    14821 ccgagaatgc tgaacggtgc gagggtgaat ttaatgccca aatgcgtgta ccagggcagc
    14881 atgaaaataa cagcgataga aaagagaaaa ttgagtagta agcgcgagga tatcttggat
    14941 aatactgaac cgtgccagac aaaaatacgg cgcagccagt gttgttgtgg acgaacaatc
    15001 atggttatct tcaggcgtgg aaaagtcgcc ctattaaacg tgattacgat cacattctca
    15061 agacactact tacaaattac ctacaagctt acaacattaa caggtgctgc atgtggcacc
    15121 tgttaatgat aaaggcgcat aggatgttgt aactaatata gtgaaataag aaacccggtc
    15181 gaaaccgggt tcagagtatt agcacaacgg acgtacagct tcgcgcattc cttttttgag
    15241 aatcgcatcc aggtcattag ccacctgctc gaccagacca ggcacttgcg tcaggtcctg
    15301 ctcccagtgg tctttttctg ccaacacaat cgctaccagt tcctgggtac cgataacacg
    15361 gtcacgatgc tggctccaga gttgctgata acgttccagc cagtgtgcat cgtcctgtac
    15421 cggatatgtt tctccattac gctcaccgcg ataaaacgca atcaatgccg ctaatgcgaa
    15481 agtcaggcgc gccggaagtg tgccgtttgc cttctgccct gccagcagct gcggcaggat
    15541 acgggtgcgg aacttggtca taccgttaag cgcgatagac agcagctgat gcttgatgta
    15601 cggattacgg aaacgtccgg tgactgcact ggcgaaagat tccagttcat cacgaggcaa
    15661 atccagtacc ggaataatct cttcgtagat ggctttttca acgaatgcgc aaatttcagc
    15721 atcgttcatc gcttcaccta cggtatccag tcctgcctgg aacgccaccg gcaccagccc
    15781 ggtgtgcgca ccgttgagga ttgccacttt gcgctcttta tacggtttaa tatcgtcaac
    15841 aatcagcacg ttgagtggat atttgtccag gcgcagctca gtcgctaagg atttcggccc
    15901 ctgaatcaca aacaggtaaa agtgttcagc ggtgtcgagg aaaccatcgt gataacccag
    15961 ttcttcttcc agtttagcca cttcatcgcg cggataaccg gtaacgatac ggtccaccag
    16021 cgtagaacag aagctgttag cctgatccag ccattgaata aatgcttctg gcagtgccca
    16081 ctcttgcgca tagcgcagaa ccagttcacg caacgcgtcg ccattgtagt cgatcaactc
    16141 acacgggatg atgatccaac ctttatccag cgcaccgttg aaatggctaa aacgttcgaa
    16201 cagcagacgg gtcagttttg ccggataact taccgctggc gcgtcatcga atttatcgcc
    16261 cgcatggtag ctgatacccg cttcggtggt gttagagaaa acaaagcgca tttccgggtt
    16321 gtgcgccagt ttcaggaatt catcgtattc actgtagacg ctgatttcac gattaacaga
    16381 gcgaatcaga cgcgcgtcgc tgaccgcttc ccccttctca ttcaggccac ggataatggt
    16441 ggtgtacaga ccatcctgtg tgctcagtga cggtgggaat gaagtttcaa tcggacgaac
    16501 aacgaccacg ccagaattca gatcggtgtg ctcattcagg agatcaattt gccagtcaac
    16561 aaaggcgcgc aggaagttac cttcaccaaa ctgaatgata cgttctggat actgtgcacc
    16621 gggaaaatcg cgacgattta gtgttttcac aatgggttcc cttctgatta gtcatacaac
    16681 ctgttttaat tggtacgaca ggttagcaaa ctttaatacg ccgaacccct gttttgatca
    16741 actcctgatg attaatgagc agttttatga gaaaagtgtg gcgcggatca tggtttaatc
    16801 gaggaactac gccttttatt cgctcaataa agtggtagaa cacattccgt taaaatcgct
    16861 gtgaatgaat aaagatgtca gacaacttcc tcaccgtaac gcatagtgct ggtacggttg
    16921 cgcccatctt tcttcgagcg atacaaacat gtatcggctt cgaccatcaa tttattaaag
    16981 tcatcggtca gcgttcggta ggatgcgcaa ccactaccga cgccaatact taccgtgaga
    17041 taaagcgttt tttgttgcca ggtgaacggt tgcagttcaa cgcctttacg gattttttcc
    17101 gccattagca gaccatcgac aggattcacc gatggcactg caacagcaaa ttcttcgccg
    17161 cccattcgcg ccaccagccc cttatcaccg acaatcttct gaatatgctg ggcaaacacg
    17221 cttaacactt tatcgccaca ttcatgtcca tagttatcgt taatactttt gaaatagtcg
    17281 atatcaagca gcatgacagt cagatgttgt gtctgtttca gcgatggact tttcagcgcc
    17341 tcataaagac cggaccgtga gtaaacctga gtcagaaaat caaagtcggc tcgcagcgca
    17401 acttgcttca ttagcgaatt gatcgctgcc acgctaaaag aaaccataat tgggcatatc
    17461 gccatcgtgg caataccgag acgtgcggag aacatttgcg gaatggagaa cggcgaaccg
    17521 accgagatat caatcaccga atttgccacc agtacgattt ccaccgcacc ggtgacaaag
    17581 gtcaacagac atgttacctg cggcgtatag cgcactgcac accagattaa tgcaggcagc
    17641 gggaacgcca gactccccgc tccgccaatg actaccgagg caataaccga cacaataagc
    17701 gcgatagcgg gcatcatctg ttctgcttta aagcgcggca gcactccagg aattgccaac
    17761 gtcagcatac aaggcacgat caacacgccc gttgagaatt gctcactgaa ccagtccgca
    17821 agcaaaggcc agaaatccag actatcaata ctgaccgaac caatcgcccc gacaatagcg
    17881 cataataatg cggcaatcag acagtaatta aatagccgta aggcacttac tggctcatac
    17941 ttattttttc ccagacgctt atcccgcgcg accagtaagg cgacggtaac aataaacatc
    18001 atattggaga aattgatagc cagtgaaacc agcccccatt cggtggttat ggcatcataa
    18061 accagcatcg ccacataact gatcgcatag taatgcagtc gattaagcca gacatagcgg
    18121 gcaaacaccc ccgccattac gccattcagc ggccagaaaa gagaaagttc gtgaaccagc
    18181 cgtaactctg caccaataaa atagaacagc gtggttagca caaaaatagc gatgctatta
    18241 cgtagcagat gaccttcctg gaacaaacgg aaagttgaaa tgggctgtac atgcattaat
    18301 tatctgtcca tacaattgcg ggtggcaatt atctcaacgc aataagtaaa ttcttagaaa
    18361 ggatagcatg ttcgctgaca acggcggcaa ctaaagatat cctgggaatg ctattttatc
    18421 gatttccgta attatcatgg ctgattcatc tgtataacca gccagtggta gattaccgtc
    18481 acaatataac gttgttgctc ctcacttaac gcttccccct gaggaatgtt atgccactta
    18541 gccagacaac cgcgacaaca ggtcgcggtg gcgtgttgag cgataaaaac cggatgcccg
    18601 cgcatggggg tttgcttacc gtcgttagcc ggtaatgccg gagcaagacg tttagcgaca
    18661 aaatccgccg catgttgttc aatgactggc gcgcctttct ccagacaata ctgacgctct
    18721 ttcacaccca aacgaaagcg agagcgaaat tttgaacggg ataaacgcgc gaagagtgga
    18781 tcgagagact gcattaataa actgaacgcc ctaattgttt cgtcaattgt tcaaggacgg
    18841 caatgcccgc aagcgagtta ccggcatcat ccagttccgg actccagact gcgatggcca
    18901 tttcatgcgg aacaatcgcc acaataccgc caccaacgcc agattttgcc ggtagtccca
    18961 cccgccaggc aaactccccc gcgttctggt acataccgct ggtcgccatc agcgcgttaa
    19021 tttgccgcgc ctgcattggc gtcaccacag gttcatcaat atgaatagct ttcccctgat
    19081 tcgccagaaa gacaaacgtc cgggccagct ctatacagct cattttcaga gcgcagtaat
    19141 gaaagtagtt ttgcagaacg gttgtcacgt catgatggaa attgccaaac gacttcatca
    19201 gccaggcgat agccgcattt cgcgcggaat gttcaaattc ggaacgcgct accaccgtat
    19261 cgtaggaaat atcagacaca ccacttaagc cgcgcacgac ttccagcata cgttgccgag
    19321 gtgcgcttaa tcgcccttgc aacatatcgc agaccaccag cgcaccggca ttaataaacg
    19381 gattacgcgg aataccctgc tccatttcca gttgcactaa agaattgaac ggtgatccag
    19441 acggatcttt gccgacgcgt tgccagattt cctcttcgga gtaatgacgc atggcgacaa
    19501 cgagactcag cactttggaa atagactgaa tggaaaaacg ttcttgcgcg tctccggcct
    19561 gaaaaagctg tccgtcaacg gtacagatag caatccccat tcgggaaccg tctaccgtag
    19621 ccagcgccgg aatataatcc gcgactttac cctgaccaat gagcggccgc acttgccgca
    19681 agatgttttc taaaattgca ttatccatgg cgactgccac tttctactcc tggaccgcag
    19741 gtctgaaaag acctgcgagt atatcagagc tgaatatgta gcgtcagatc cggtctttcc
    19801 acaccgtctg gatattacag aattcgtgta agccaaaatg ggaaagctca cgcccaaagc
    19861 cactcttctt cacgccacca aaagcgactc gcgcgtcgct ggcacagtaa ccattgataa
    19921 acaccccacc gcattccaga cgtgccgcca tctgtctggc ccgggtttcg tcggtggtga
    19981 aaatggtcgc tgaaaggccg aactcgctat cattagccag ttccagtgca tgttctgcat
    20041 ctttcgcaat ggtgattgcc gcaacagggc caaacatttc ttcccgaaac gcggtcattt
    20101 ctggggtaac gttcgccaga accgttggcg gatagtagtt acctgcccca gccatctttt
    20161 ccccgcccag taacaaacgc gcaccctgcg ccagggtttt ttccacctga tgatgcagct
    20221 catcacgtaa atcaaaacga gccattggtc cgagagcgtt ctcttcgtca cggggatcgc
    20281 ccattttcaa ggctgccgca gctgccacaa aacgttcggt aaatgccgaa gcaattccct
    20341 cttcgataat aaagcgtttt gccgccgcac aaacctgtcc ggtattctga taacgtccgg
    20401 ctaccgccgc tttaaccgcc agttccagat cggcatcgtt aagcacaata aatggatccg
    20461 aaccgcccag ttccagtacg cattttttca gtgccgctcc agcctgtgcg ccaatagccg
    20521 ctcccgcacg aacacttccg gtcactgtga cagcagcaat gcgcgaatct ttaatcatct
    20581 gactgacacc gttgttgtca gcattcagcc agccatatac gccttgcggg atacccgcat
    20641 ctttaaacac ctgggcaatg agctgtgcac agcccatcac attcggcgca tgtttaagta
    20701 agtagccgtt acctgcaaga atgatgggaa ccgcgccacg catcacctgc cataacggaa
    20761 aattccacgg cataatcgcc agaattgtcc ccaacggtcg atactcaata actgcctgct
    20821 gattttccac cagcgtaggt tccgccttca gcattgccgg accatgttct gcataccagt
    20881 cacacaaatt cgccgatttc gccacttcag cgcgcgcctg gttgattggt ttgcccattt
    20941 cacgggtgat catttgcgcc atttcttcgc tacggacacg cagagcctta ccgatatcac
    21001 gcagtttttc agcacgataa tctatatttg tctcgcgcca gtcgcgaaag cctgctgccg
    21061 ccagctgaag tgcgttttcg atatcatcag cgccagccca cggcagcaca gaaagttgtt
    21121 caccggtggc aggatttatc gaaattgcat gagttgccgg agtaatggtc atcggggtat
    21181 ctcctttatg agttatggta aaaagatacg cagatttact cttgctttaa aatgaataat
    21241 attaagccac ttattcacga atcgagaata ttatggatct gacccaactg gagatgttca
    21301 acgcggttgc cgaagctggc agcattacgc aggctgcaac aaaagtgcat cgcgtgccgt
    21361 cgaatctcac tacccgtcta cgccagctgg aaacagaact gggggttgat ctgtttattc
    21421 gcgagaatca gcgtttacgt ctctcgcctg ccgggcataa ctttttacgc tatagccaac
    21481 aaattctcgc gttagtggat gaagcgcgga gcgttgtcgc tggcgatgag ccgcaaggtt
    21541 tattttctct tggttcgctg gaaagcaccg cagcagtgcg cattccggcc atgctggcgg
    21601 aatttaaccg tcgttatccc aaaattcagt tttcactttc caccggccct tccggcacta
    21661 tgctggaagg ggtgctggag ggaaaactga atgcggcgtt tattgatggg cccattaacc
    21721 atactgccat tgacgggata ccggtgtacc gcgaggagct gatgattgtc acgccacaag
    21781 gacatgcgcc agtaacccgt gccagtcagg ttaatggcag taacatttat gccttccgcg
    21841 ccaattgttc gtatcgtcgc cacttcgaga gctggtttca tgctgacggt gccgctccgg
    21901 gaactattca cgagatggag tcgtatcacg ggatgttggc ctgtgtggtc gcaggagcag
    21961 gcattgcgct tattccacgc tcaatgctgg aaagtatgcc ggggcatcac caggttgaag
    22021 cgtggccgtt agctgagcaa tggcgttggt taacaacctg gctggtctgg cgtcgcggtg
    22081 cgaaaacgcg tccgcttgag gcatttattc aactactgga tgtgtctgac tcggcaaaac
    22141 agagttagta atgagctatt tttgatagtt cttgcggcta atatgctcta tatagtgatg
    22201 ctccgatgac ttatgactat atggggcaaa tatggttacg ccagtaagca tcagcaattt
    22261 catctcactt cctgatgatt ttcccgttcg taatattgca cctcaggtaa aagaagaaca
    22321 ggcccccttc ctgatcgtca ttaaaatgtg atgttaacaa ctctctgatg cgtgacagaa
    22381 caaccgtcag agagatggaa atgcgatcca gatcacaaat gcattgtatt cacatcatta
    22441 accgttttaa gatcatttca tcactttttc gcaactcacc cgataatctg ttatgacaac
    22501 aaacactgtt tcccgcaaag tggcgtggct acgggtcgtt acgctggcag tcgccgcctt
    22561 catcttcaac accaccgaat ttgtcccagt tggcctgctc tctgacattg cgcagagttt
    22621 tcacatgcaa accgctcagg tcggcattat gttgaccatt tacgcatggg tagtagcgct
    22681 aatgtcattg ccttttatgt taatgaccag ccaggttgaa cggcgcaaat tactgatctg
    22741 cctgtttgtg gtgtttattg ccagccacgt actgtcgttt ttatcgtgga gttttaccgt
    22801 tctggtgatc agtcgcattg gtgtggcttt tgcacatgcg attttctggt cgattacggc
    22861 gtctctggcg atccgtatgg ctccggccgg gaagcgagca caggcattga gtttaattgc
    22921 caccggtact gcactggcga tggtcttagg tttacctctc gggcgcattg tggggcagta
    22981 tttcggttgg cgaatgacct tcttcgcgat tggtatcggg gagcttatca cccttttgtg
    23041 cctgattaag ttacttccct tactgcccag tgagcattcc ggttcgctga aaagcctccc
    23101 gctattattc cgccgcccag cattgatgag catttatttg ttaactgtgg tggttgtcac
    23161 cgcccattac acggcataca gctatatcga gccttttgtg caaaacattg cgggattcag
    23221 cgccaacttt gccacggcat tactgttatt actcggtggt gcgggcatta ttggcagcgt
    23281 gattttcggt aaactgggta atcagtatgc gtctgcgttg gtaagtacgg cgattgcgct
    23341 gttgctggtg tgcctggcac tgctgctacc tgcggcgaac agtgaaatac acctcggggt
    23401 gctgagtatt ttctggggga tagcgatgat gatcatcggg cttggtatgc aggttaaagt
    23461 gctggcgctg gcaccagatg ctaccgacgt cgcgatggcg ctattctccg ggatatttaa
    23521 tattggaatc ggcgcgggtg cgttggtagg taatcaggta agtctgcact ggtcaatgtc
    23581 gatgattggt tatgtgggcg cggtgcctgc ctttgccgcg ttaatatggt caatcatcat
    23641 tttccgccgc tggccagtga cactcgaaga acagacgcaa tagttgaaag gcccatttgg
    23701 gcctttttta gtggtacgtt ttaatgattt ccaggatgcc gttaataata aactgcaccc
    23761 ccatacatac cagcaggaat cccatcagac gggagatcgc ttcaatgccc cccttaccca
    23821 ccagccgcat aattgcgcca gagctgcgta gacttcccca caaaataacc gccaccagga
    23881 aaaagatcag cggcggcgca accatcagta cccaatcagc aaaggttgaa ctctgacgca
    23941 ctgtggacgc cgagctaata atcatcgcta tggttcccgg accggcggta cttggcattg
    24001 ccagtggtac aaaagcaata ttggcgctgg gttcatcttc cagctcttcc gacttgcttt
    24061 tcgcttctgg tgaatcaatc gctttctgtt gcggaaagag catccgaaaa ccgataaacg
    24121 cgacgattaa gccgcctgca attcgcagac cgggaatcga aatgccaaat gtatccatca
    24181 ccagttgccc ggcgtaatac gccaccatca tgatggcaaa gacgtacacc gaggccatca
    24241 acgactgacg attacgttcg gcactgttca tgttgcctgc caggccaaga aataacgcga
    24301 cagttgttaa tgggttagct aacggtagta acaccaccag ccccaggcca attgctttaa
    24361 ataaatctaa cattggtggt tgttatcctg tgtatctggt ttatcagcga aaagtataag
    24421 gggtaaacaa cgataaagtg tcactcttta gctaaccttg catcgcattg accaaaactt
    24481 gaaccgattt agcaaaacgt ggcatcggtc aattcattca tttgacttat acttgcctgg
    24541 gcaatattat cccttgcaac taattacttg ccagggcaac taatgtgaaa agtaccagcg
    24601 atctgttcaa tgaaattatt ccattgggtc gcttaatcca tatggttaat cagaagaaag
    24661 atcgcctgct taacgagtat ctgtctccgc tggatattac cgcggcacag tttaaggtgc
    24721 tctgctctat ccgctgcgcg gcgtgtatta ctccggttga actgaaaaag gtattgtcgg
    24781 tcgacctggg ggcgctgacc cgtatgctgg atcgcctggt ctgtaaaggc tgggtggaaa
    24841 ggttgccgaa cccgaatgac aagcgcggcg tactggtaaa acttaccacc agcggcgcgg
    24901 caatatgtga acaatgccat caattagttg gccaggacct gcatcaagaa ttaacaaaaa
    24961 acctgacggc ggacgaagtg gcaacacttg agcatttgct taagaaagtc ctgccgtaaa
    25021 caaaaaaaga ggtatgacga tgtccagacg caatactgac gctattacca ttcatagcat
    25081 tttggactgg atcgaggaca acctggaatc gccactgtca ctggagaaag tgtcagagcg
    25141 ttcgggttac tccaaatggc acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt
    25201 aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa
    25261 cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa ctctgacccg
    25321 aaccttcaaa aattactttg atgttccgcc acataaatac cggatgacca atatgcaggg
    25381 tgaatcgcgc tttttacatc cattaaatca ttacaacaac tagttgataa cgtgacaacg
    25441 tcactgaggc aatcatgaaa ccacttttat ccgcaatagc aactgcgctt attctctttt
    25501 ctgcgcaggg cgttgcggaa caaaccacgc agccggttgt tacttcctgt ggcaatgtcg
    25561 tggttgttcc cacatcgcag gaacaaccac cgtttgattt aaatcacatg ggtactggca
    25621 gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg tagtttgttc
    25681 tggccccgac atatcggggc ttattaactt cccactttta ccgctttacg ccaccgcaag
    25741 ccaaatacat tgatatacag cccggtcata atgagcaccg caccgaaaaa ttgtagaccc
    25801 gttaagcgtt catccaataa tagtgccgca cttgccagtc ctactacggg caccagtaac
    25861 gataacggtg caacccgcca ggtttcatag cgtcccagta acgtccccca gatcccataa
    25921 ccaacaattg tcgccacaaa cgccagatac atcagagaca agatggtggt catatcgata
    25981 gtaaccagac tgtgaatcat ggttgcggaa ccatcgagaa tcagcgaggc aacaaagaag
    26041 ggaatgattg ggattaatgc gctccagatt accagcgaca tcaccgccgg gcgcgttgag
    26101 tgcgacatga tctttttatt gaagatgttg ccacacgccc aactaaatgc tgccgccagg
    26161 gtcaacataa agccgagcat cgccacatgc tgaccgttca gactatcttc gattaacacc
    26221 agtacgccaa aaatcgctaa ggcgatcccc gccaattgtt tgccatgcag tcgctccccg
    26281 aaagtaaacg cgccaagcac gatagtaaaa aacgcctgtg cctgtaacac cagcgaagcc
    26341 agtccagcag gcataccaaa gttaatggcg caaaacagaa aggcaaactg cgcaaaactg
    26401 atggttaatc cataccccag cagcaaattc agtggtactt tcggtcgagc gacaaaaaag
    26461 atggccggaa aagcgaccag cataaagcgc aaaccggcca gcatcagcgg tggcatgtta
    26521 tgaagcccca ctttgatgac cacaaaattc agcccccata cgaccactac cagtagcgcc
    26581 aacaccccat cttttcgcga cattataccg cctctgaatt tcatcttttg taagcaatca
    26641 acttagctga atttactttt ctttaacagt cgattcgtta gtcaccgttt acgacggcat
    26701 taatgcgcaa ataagtcgct atacttcgga tttttgccat gctatttctt tacatctcta
    26761 aaacaaaaca taacgaaacg cactgccgga cagacaaatg aacttatccc tacgacgctc
    26821 taccagcgcc cttcttgcct cgtcgttgtt attaaccatt ggacgcggcg ctacgctgcc
    26881 gtttatgacc atttacttga gtcgccagta caacctgagt gtcgatctaa tcggttatgc
    26941 gatgacaatt gcgctcacta ttggcgtcgt ttttagcctc ggttttggta tcctggcgga
    27001 taagttcgac aagaaacgct atatgttact ggcaattacc gccttcgcca gcggttttat
    27061 tgccattcct ttagtgaata acgtgacgct ggtggtgctc ttttttgccc tcattaactg
    27121 cgcctattcc gtttttgcta ccgtgttgaa agcctggttt gccgacaatc tttcgtccac
    27181 cagcaaaacg aaaatcttct caatcaacta caccatgctc aacattggct ggaccatcgg
    27241 tccgccgctc ggcacgttgt tggtgatgca gagcatcaat ctgccctttt ggctggcagc
    27301 tatctgttcc gcgtttccca tgcttttcat tcaaatttgg gtaaagcgca acgagaaaat
    27361 catcgccacg gaaacaggca gtgcctggtc gccgaaagtt ttattacaag ataaagcact
    27421 gttgtggttt acctgctctg gttttctggc ttcctttgtt agcggcgcat ttgcctcgtg
    27481 catttcacaa tatgtgatgg tgattgctga tggtgatttt gccgaaaagg tggttgcggt
    27541 tgttcttccg gttaatgctg ccatggtagt tacgttgcaa tattccgtgg gccgccgact
    27601 taacccggct aacatccgcg cgctgatgac agcaggcacc ctctgtttcg tcattgggct
    27661 ggtcggtttt attttttccg gcaacagcct gctattgtgg ggtatgtcag ctgcggtatt
    27721 tactgtcggt gaaatcattt atgcgccggg cgagtatatg ttgattgacc atattgcgcc
    27781 gccaggaatg aaagccagct atttttccgc tcagtcttta ggctggcttg gtgcggcgat
    27841 taacccatta gtgagtggca tagtgcttac cagtctgccg cctttctcgc tgtttatcat
    27901 cttagcgttg gtgatcattg ctgcgtgggt gctgatgtta aaaggaattc gtgcaagacc
    27961 gtgggggcag cccgcgcttt gttgatttaa gtcgaacaca ataaaaattt aattcagcct
    28021 tcgtttaggt tacctctgct aatatctttc tcattgagat gaaaattaag gtaagcgagg
    28081 aaacacacca caccataaac ggaggcaaat aatgctgggt aatatgaatg tttttatggc
    28141 cgtactggga ataattttat tttctggttt tctggccgcg tatttcagcc acaaatggga
    28201 tgactaatga acggagataa tccctcacct aaccggcccc ttgttacaac tgtgtacaag
    28261 gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca
    28321 tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg ataacggatc
    28381 gttgacacag tagcatcagt tttctcaatg aatgttaaac ggagcttaaa ctcggttaat
    28441 cacgttttgt tcgtcaataa acatacagcg atttcttccg gtttgcttac cctcatacat
    28501 tgcccggtcc gctcttccaa tgaccacatc cagaggctct tcaggaaatg cgcgactcac
    28561 acctgctgtc acggtaatgt tgatatgccc ttcagaatgt gtgatggcat ggttatcgac
    28621 taactggcaa attctgacac ctgcacgaca tgcttcttca tcattagccg ctttgacaat
    28681 aatgataaat tcttcgccac cgtagcgata aaccgtttcg taatcgcgcg tccaactggc
    28741 taagtaagtt gccagggtgc gtaatactac atcgccgatt aaatgcccgt aggtatcatt
    28801 aaccaattta aatcggtcaa tatccaacaa cattaaataa agattcagag gctcagcgtt
    28861 gcgtaactga tgatcaaagg attcatcaag aacccgacga cccggcaatc ccgtcaaaac
    28921 atccatattg ctacggatcg tcagcaaata aattttgtaa tcggttaatg ccgcagtaaa
    28981 agaaagcaac ccttcctgaa aggcgtcgaa atgcgcgtcc tgccagtgat tttcaacaat
    29041 agccagcatt aattcccgac cacagttatg catatgttga tgggcagaat ccattagccg
    29101 aacgtaaggt aattcatcgt tatcgagtgg tcccagatga tcaatccacc gaccaaactg
    29161 gcacagttca taagaatggt tatccgttat ttctggctta ctggcatctc tcgcgaccac
    29221 gctgtgaaac atacttacca gccactggta gtgggcatcg atagccttat tgagatttaa
    29281 caagatggca tcaatttccg ttgtcttctt gatcattgcc actccttttt cacagttcct
    29341 tgtgcgcgct attctaacga gagaaaagca aaattacgtc aatattttca tagaaatccg
    29401 aagttatgag tcatctctga gataacattg tgatttaaaa caaaatcagc ggataaaaaa
    29461 gtgtttaatt ctgtaaatta cctctgcatt atcgtaaata aaaggatgac aaatagcata
    29521 acccaatacc ctaatggccc agtagtttag gccatcaggc taatttattt ttatttctgc
    29581 aaatgagtga cccgaacgac ggccggcgcg cttttcttat ccagactgcc gctaatgttg
    29641 atcatctggt ccggctgaac ttctcgtcca tcaaagactg ccgcaggaat aacaacatta
    29701 atttcaccac tcttatcgcg aaaaacgtaa cggtcctctc ctttgtgaga aatcaaatta
    29761 ccgcgtagtg aaaccgaagc gccatcgtgc atgttttttg cgaaatcaac ggtcattttt
    29821 tttgcatcat cggttccgcg atagccatct tctattgcat gaggcggcgg tggcgctgca
    29881 tcctgtttta atccgccctg gtcatctgcc aacgcataag gcatgacaag aaaacttgct
    29941 aatacaatgg cctgaaattt catactgact ccttaattgc gtttggtttg acttattaag
    30001 tctggttgct atttttataa ttgacaaata agaatactgc caattgttat aaggcattta
    30061 aaatcaggca actagctgtc aaatattcag agaattttac tcactaaagt aagaagaaga
    30121 ttaaagagtc ttaaacatat ttcagaacaa tcagatttat aaatttgaaa attattataa
    30181 cggaagggta tacaaaaaaa gtaattttgc tggcgactgc gaaacggcat tcgcattagc
    30241 ggtgaagttt gccctcaccc agcggctgca attactgcta taacgaggct ggtctggcga
    30301 taaatccagg ccagccattg gtggtgttta tacgttcaag ccacgatgtt gcagcatcgg
    30361 cataatctga ggtgccttac cgcgccattg tcgatacagg cgttccagat cttcgctgtt
    30421 acctctggaa agaatcgcct cgcgaaaacg ccgcccattt tcacgcgtta atccgccctg
    30481 ctcaacaaac cactggtaac catcatcggc caacatttgc gtccacagat aagcgtaata
    30541 acctgcggca tatccgccac caaaaatatg ggcgaaataa ctgctgcgat agcgtggcgg
    30601 tatagcagga agatccatat tttccgccac cagcgcccgc aattcaaaat catcgacatc
    30661 ctgcattgct tcgttttctt ccaggcaatg ccagcgcata tcgagaagtg cggcgctaag
    30721 cagttcgctc atctcatacc ctttattgaa caggctggca ttacgcattt tctgttgcag
    30781 ttcatcaggc attgctgccc cgctctgata atgccgggcg tagcgagcga atacctgcgg
    30841 atgcgttgcc cagtgttcgt tgatttgcga cggaaattcg acaaaatcac gcggcgtgtt
    30901 ggtgccggaa agcgtggcat aacgctggcg ggcaaaaagg ccgtgcagcg tatgaccaaa
    30961 ttcatggaat aaggttatga catcatccca gagtaacaac gcaggctccc cagcggcgga
    31021 tttctgataa ttgcagacgt tataaattac cggatgcgtt tcattcagcg ttgattgctc
    31081 aacaaaattg cccatccatg caccgccgct ttttgaatca cgggcgaaga aatcaccgta
    31141 aaataacgcc agccccacgc cattatgatc aaaaatttcc cacacacgaa cgtcaggatg
    31201 gtagacagga atatcaaaac gttcgacaaa cttaataccg aagagctgat tcgcggtcca
    31261 gaatacacct tcattcaaca ccgtgtttaa ttcaaaatat ggcttgagct gcgattcatc
    31321 gagatcgaat ttctcacgtc ttacctgctc ggcataaaat gcccagtccc acggctgcgc
    31381 gctaaacccg ccctgctgct tatcaataac cgcctgtatg gagactaatt catcgctcgc
    31441 acgttgacgc gccgctggaa caatttcccg cataaagttg agtgctgctt ctggcgtttt
    31501 tgccatctga tcggcgattt tccatgcggc ataatgaggg aaaccaagca gtttcgcctg
    31561 ctgcgcgcgg atctccacca gacgttgaat gatagcgcgg gtatcattgg catcattttt
    31621 ttccgctcgc gtccagcccg cagtgaacag tttttcacgc gtcgcgcgat cgcgcagttc
    31681 agcaagcgct ggttgctggg tggtattcag caacggaatc agccatttgt tatccagacc
    31741 tttctcgcga gccgcctctg ccgccagcgc aatctcttgc tcactcattc ctgccagctg
    31801 cgcgaaatcg ttcacaacca gaccgccgga tttatttgct gccagtaacc gctggttaaa
    31861 ctggctggtc agggtcgcag cttctgtatt cagtactttt aattttgctt tatcagcttg
    31921 tgcaagtttg gctccggcaa ggacaaaacg ttgatgaatg acctccacca ggcggatgga
    31981 ttcactatca agtcccaggg attcacggcg ttgccagaca gcatctaccc gcgcgaataa
    32041 ttcaccgttc agatagatat cattagccag ttccgccagt tcagcggaaa actgttcatc
    32101 aagacgctgt aattcatcat tagtatgcgc cgcagtcatc gcaaaaaaga cgctggtaac
    32161 gcgggtaagt aattctccgc tttgttccag tgccagaata gtattgttga aatcaggcgc
    32221 ttgcgggtta agcgcgatgg cagcaatttc tgcccgcttt tgctgcattc cctcatcgaa
    32281 tgccgggcga tagtgatgat tggcaatttg atcaaaatgg ggagccagat acggcagtgt
    32341 gctttgcaca aggaaaggat tcattgttgt cattttcttc tcctgaacgc gaggtgttcc
    32401 atagcgtagg cttactgata acggagtgca atcttgcaaa ccagtattac ccgctcttaa
    32461 gcatcacgtg ctatgttatt gacacacaaa agcgttgagg aacagtgaga tgatcgtttt
    32521 agtaactgga gcaacggcag gttttggtga atgcattact cgtcgtttta ttcaacaagg
    32581 gcataaagtt atcgccactg gccgtcgcca ggagcggttg caggagttaa aagacgaact
    32641 gggagataat ctgtatatcg cccaactgga cgttcgcaac cgcgccgcta ttgaagagat
    32701 gctggcatcg cttcctgccg agtggtgcaa tattgatatc ctggtaaata atgccggcct
    32761 ggcgttgggc atggagcctg cgcataaagc cagcgttgaa gactgggaaa cgatgattga
    32821 taccaacaac aaaggcctgg tatatatgac gcgcgccgtc ttaccgggta tggttgaacg
    32881 taatcatggt catattatta acattggctc aacggcaggc agctggccgt atgccggtgg
    32941 taacgtttac ggtgcgacga aagcgtttgt tcgtcagttt agcctgaatc tgcgtacgga
    33001 cctgcatggt acggcggtgc gcgtcaccga catcgaaccg ggtctggtgg gtggcaccga
    33061 gttttccaat gtccgcttta aaggcgatga cagtaaagcg gaaaaaacct atcaaaatac
    33121 cgttgcattg acgccggaag atgtcagcga agccgtctgg tgggtgtcaa cgctgcctgc
    33181 tcacgtcaat atcaataccc tggaaatgat gccggttacc caaagctatg ccggactgaa
    33241 tgtccaccgt cagtaatttt tatacccggc gtaactgccg ggttattgct tgtcacaaaa
    33301 aagtggtaga ctcatgcagt taactcactc acaagcaaga acgaatgacc gtcgaaacgc
    33361 aacttaatcc cacacagcct gtcaatcagc agatttatcg tattcttcgt cgcgacattg
    33421 tccattgcct gattgcacca ggcacaccgt tgtcggaaaa agaagtttct gttcgtttca
    33481 atgtgtcacg ccagccggtt cgtgaagcct ttattaaact ggcggaaaac ggcctgattc
    33541 aaattcgtcc gcagcgtggc agctacgtca acaaaatttc catggcccag gtgcgcaacg
    33601 gcagttttat ccgtcaggcc attgagtgcg cggtggcgcg tcgggcggcg agcatgatta
    33661 ccgaaagcca gtgctaccaa ctggaacaaa atcttcacca gcaacgcatt gccatagagc
    33721 gcaagcaact ggatgatttt tttgaacttg atgacaactt ccatcaactc ctgacgcaga
    33781 ttgccgactg tcaactggcg tgggatacca ttgagaacct gaaagcgacc gttgatcgcg
    33841 tgcgctatat gagtttcgac cacgtttctc caccagaaat gctgttacgc cagcatcttg
    33901 agattttctc tgccctgcaa aaacgtgatg gcgatgcggt agaacgtgca atgacgcacc
    33961 atttgcagga aatcagcgaa tccgtgcgcc agatccgtca ggaaaacagc gactggttta
    34021 gcgaagagta attcatttcc tctcatccca tccggggtga gagtttttcc ctccgtcata
    34081 tggctcatgc atgcatcaaa aaagatgtga gcttgatcaa aaacaaaaaa tatttcactc
    34141 gacaggagta tttatagtgc gcccgttacg tgggcttcga ctgtaaatca gaaaggagaa
    34201 aacacctatg acgacctacg atcgtaaccg taacgcaatc accactggca gccgtgttat
    34261 ggttagcggc accggtcaca ctggcaagat cctgtcgatt gatactgaag gtctgaccgc
    34321 tgagcaaatc cgccgcggaa aaaccgtagt tgttgaaggt tgtgaagaga aactggcacc
    34381 tctggacctg attcgtctcg gcatgaacta agcgtgtgaa tgccgccgat ggcggcattg
    34441 ctttttttac ttcacggaat atttcgccac ggtcgctttc gcaccatgcg ctaataaaga
    34501 caagtacgct tccgtcactt ttgcagtaaa caagctattg tctggcaaat catcaccaaa
    34561 gatcgcctta atcgccagca atgactggac gcgcgctttc ccttcggcac tactttgtac
    34621 agccttctga ataacaggta acagtgggtc actgatttct atcggatttc cctgttcatc
    34681 aacaccaccg acataacgca tccaacctgc gacgcccagc gccagcagat cgaacttgct
    34741 gtcatgcgcc agatgccagc gaacagaatc caacatccgc tgtggcaatt tctggctacc
    34801 atccatcgca atctgccagg ttcgatgacg tagcgccggg ttgctatagc gttcaattaa
    34861 tcggttagcg taatcttgca aatcaacgcc ctgcactttc aacgtcggcg cttgttcctg
    34921 caacatcaag gcatacgctg catgacgata atgttcatct tccatgcagt cattaatgtg
    34981 ctgatatccg gcaagatacc ccagatacgc caggaatgaa tgactgccgt tgagcatgcg
    35041 caacttcatc tcttcataag gcagcacatc actaaccagt tcggctcccg ctttttccca
    35101 ttccggacgt ccggcaacaa agttatcttc tattacccac tggcggaaag gttcacaggc
    35161 aacgcccgca ggatcgcgca caccggtaag ttgttcgatt ttcgccagcg tatcctccgt
    35221 cactgcgggc acaatacggt ccaccattgt tgatgggaaa gtcacgtttt cttcgatcca
    35281 ttgtgccagt tttacatcaa cagcttgcgc gtaggaagtg acaacgtcac gcataacatg
    35341 accgttttct ggcatgttgt cacatgacat gacggtaaat gcgggaagtc ctgccgcttt
    35401 acggcgagcc agcgcctcga caatcacccc tgttgcagtt ttcggctggt ggggattttg
    35461 tacgtcggca acgaccatcg ggtgatcgag cattaactgt ccggtcgccg gagagtggaa
    35521 ataccctttt tcggtgattg tcagagagac aatcgcgatt tgcggttcac acatcacagc
    35581 caacacggtt tctaagccat caatctgtac gtgcaaggct tttttaacga cgccaacgac
    35641 gcgagccgtc cacgcatcgg ccgacatttc cgcaacggta taaagattat cttgctgttg
    35701 taaatcggca atttgctgtt cgccgccgat taaattgacc tcataatatc cccagtcact
    35761 gaaatgttcc gtagcaagaa tatcggcata cacaccctga tgcgcacggt gaaatgcacc
    35821 aaagcctaaa tgaacaattc ttggggccag gttattacga tcataaacag ggagcgtcgc
    35881 ttttgctgat aacaaattat ttcccataac aattccttaa atttaaatat ggcaagctgc
    35941 atgttttgtt atatgaataa aaaatcccct ctccggtaag agaagggatg gaggatttac
    36001 agacttcttg aaggttgcgc agctgttaca acacgcggtt gatcttccgc ggcatcttcc
    36061 agtgcactta aatcacggtc tttcacttct ggcattttca gcgcagagat taaaccaatc
    36121 actgaatatg ccatgatcat aatggcgatc ggataccagg attccgtcat ggtgcagaaa
    36181 atacccgcca ggataggacc aaaaccggaa gcgataagac caccaatttc tttagaaatg
    36241 gccatccggg taaagcggtt tttacagccg aacatttctg ccatggtaat gttttccaga
    36301 gcaaataatc ccagcaccgc acagttatga atcacaatca gtgccaccat aatggtgctc
    36361 ggggcatagc ttttatctac gatgatagaa agcattggcc atgccagcac aatcgcggag
    36421 gtattcataa taatatacgg gatccggcga ccaattttat cggataacca accaaggaac
    36481 ggaatggtca taaagccgag aatcgaactg atcatcaatg catctgttgg aattgctttg
    36541 ttaaacaata acgtctgtac taaatagcct gcaaggaaag tctgaattaa accggagtta
    36601 ccagcctgac caaaacgcag ccctgttgcc agccagaagg atttgctctg gaacatgcta
    36661 ccagcaggtg caggttttgc tgtcggttgg ttgctgtcgt taaccttctc aaagaccggg
    36721 ctttctttca gattcatacg caaccagata gcaaagacca tcacgacaac gctcgccagg
    36781 aacggtatac gccatcccca cgccaacagt tcctctttac tgagaatgaa gaacataaag
    36841 gcccagattg ccgttgcgct taaggttccg cagttagttc ccatagccac aaatgaggag
    36901 ataattccgc gcttaccttt tggcgcatat tccgccagca tcgtaccggc accggaaatt
    36961 tccgcacctg cacccaatcc ctgaataata cgcagcgtca ccagcaagat gggtgcaaaa
    37021 acaccaatct gtgcataggt cggtaacaca ccaattaagg tggtacagat ccccatcatg
    37081 gtgatggtaa taaagagtac ttttttacgc cctattctgt cgcccatttt gccgaaaata
    37141 aatgctccga caatacgcgc cacataacct gcaccgtagg ttcccattgc cagaattaac
    37201 gccattgccg ttgatgattc aggaaaaaat atttcatgaa acactaacgc tgcaccgagc
    37261 gaatataact ggaaatccat aaattcaagc gcagtgccca gccatcccga tactgctgct
    37321 ttcaccaaat ccttagtgct tctttcgtgt ttttctattg tcataatggt tatctctaaa
    37381 aaagaggtaa gatgcgtact acttactcgc cgttattggt attattcaga aaaagtaagt
    37441 aagactttgc agcaatgttt ttgatcctgt tcaaataaac taatggcatc agcaacatgc
    37501 tggaaatcaa acgtatgggt aattaatttt tctggtttaa ttaacccttt acttaaccag
    37561 tcgataacga ccggaaattt atttgcattt aagcgtgaag agaaaataga gagttctttt
    37621 ccggtaattc cttgctgaat cacttcagac ggttcactgg agaagcccat caatacaata
    37681 cgtgccgctg gagaagccag cgttacagcc tctttcagga tagaaggatg acaagccgca
    37741 tcgataatta atgtcggctt gatgcctttt tcagcgaaac tctcgccaag cggtgtctgg
    37801 ctgttattaa tcgcccagtc tgccccgctc tctttcgctt tttccagtcg ttcatcaatg
    37861 cgatcggcaa caatcacatt tttaacgtta tagacgcctt ttaatacctg aacgatcgtc
    37921 aggccgattg gaccggcacc gtaaaccaga acggtatcat tttcagtcgg ttgaccatga
    37981 ccggtaacgt tagccgcaat ggtaaaaggt tcgatcatca ccgcatattg atcggccact
    38041 gcttcaggaa ttttccacgc attttttgcc ggaaccacgg catattcact gaaaccaccg
    38101 tcagcgtgca cacctaatac agccagtgtc gtacaaacgt tcggcttacc tatagagcac
    38161 ggatagcaat gcccacagct gaccaccgga tcgacagcaa cgcgttcacc gactctggcg
    38221 ctttccacgc cttcacccac tgcatcaatg acgccaaaga attcatgacc aatgacgcgc
    38281 ggatatttcg caaaaggatt atgcccacga taaatatggc tatctgaacc acaaattccg
    38341 gcaagtttca cttttactcg tacttcaccc gctgacgggg tgggtatttc gcgttcgata
    38401 attgccagtt gattcggttt ttcaattaaa atgcttttca ttatcttact ccttaccagt
    38461 tccacagcgt gccatcttcc agacgtgcga ctggcagata agcaggttca tagggatatt
    38521 tcgccgccag cttttcatcg aattcgatgc caagacccgg tttgtctccc ggatgcatat
    38581 agccgttatc gaaagtccag ttgtgcggga agacttcaag catttgttcg gaataaccca
    38641 tgtactcctg gacaccgaaa ttggggaccc acagatcaaa gtgcagcgcc gcagccatgc
    38701 agactggcga caaatcggaa ggaccgtgtg agccagtacg tacctgatac agcgaagcaa
    38761 aatcggcaat ccggcgcata ccggtaatcc cccccgcgtg agtcagcgtg gtgcggatat
    38821 agtcgataag ttgttcttca atcagctgtt tgcagtccca gatgctgttg aagacttcac
    38881 ccactgcaat tggcgtgacg gtgtgttggc gaatcaggcg gaaacactcc tggttttccg
    38941 caggcgtcgg gtcttccatc cagaacatgc gataatcttc aatgctttta ccaaagcgcg
    39001 ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag caaatgttca ttaaaaccaa
    39061 acttgttacg taccgcgtca aacaatttcg gcatgaaatc gaggtatttc tccgtcgacc
    39121 acagctgctc ttccggccac tgtcctttgg ttgcgggttc ataagccaga cctttacctt
    39181 tcgacatgcc ataggtggtt ttcataccag ggattccgca ctgcacgcgg atggctttga
    39241 atcccagctc ctgatgacgg gcataatcat ccagagcttc atcaatactg tgaccggtgg
    39301 tatggcaata aaccatcacc ccttcacgag acgcgccgcc gagtaactgg taaagcggca
    39361 tgttggcagc tttggcttta atatcccaca gcgccatatc aaccgctgaa atggccgaca
    39421 tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa aaactgccag atatcttcga
    39481 tacggtgtgc atcgcgacca ataagctgcg gacaaaggtg atcctgcaaa ta
//
LOCUS       DAZKJR010000028        38471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKJR010000028 DAZKJR010000000
VERSION     DAZKJR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.028"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..1829
                     /gene="aslA"
                     /locus_tag="ACHOWS_001511"
     CDS             174..1829
                     /gene="aslA"
                     /locus_tag="ACHOWS_001511"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFQ0047110.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1987..3222)
                     /gene="aslB"
                     /locus_tag="ACHOWS_001512"
     CDS             complement(1987..3222)
                     /gene="aslB"
                     /locus_tag="ACHOWS_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFQ0047111.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKVFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3369..3445)
                     /locus_tag="ACHOWS_001513"
     tRNA            complement(3369..3445)
                     /locus_tag="ACHOWS_001513"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3409..3411),aa:Pro,seq:tgg)
     gene            complement(3488..3574)
                     /locus_tag="ACHOWS_001514"
     tRNA            complement(3488..3574)
                     /locus_tag="ACHOWS_001514"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3538..3540),aa:Leu,seq:cag)
     gene            complement(3595..3670)
                     /locus_tag="ACHOWS_001515"
     tRNA            complement(3595..3670)
                     /locus_tag="ACHOWS_001515"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3635..3637),aa:His,seq:gtg)
     gene            complement(3729..3805)
                     /locus_tag="ACHOWS_001516"
     tRNA            complement(3729..3805)
                     /locus_tag="ACHOWS_001516"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3769..3771),aa:Arg,seq:ccg)
     gene            complement(3908..5293)
                     /gene="yifK"
                     /locus_tag="ACHOWS_001517"
     CDS             complement(3908..5293)
                     /gene="yifK"
                     /locus_tag="ACHOWS_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0047112.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEK"
     gene            complement(5484..6224)
                     /gene="wecG"
                     /locus_tag="ACHOWS_001518"
     CDS             complement(5484..6224)
                     /gene="wecG"
                     /locus_tag="ACHOWS_001518"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFQ0047113.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6227..7579)
                     /gene="wzyE"
                     /locus_tag="ACHOWS_001519"
     CDS             complement(6227..7579)
                     /gene="wzyE"
                     /locus_tag="ACHOWS_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFQ0047114.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7576..8655)
                     /gene="rffT"
                     /locus_tag="ACHOWS_001520"
                     /gene_synonym="wecF"
     CDS             complement(7576..8655)
                     /gene="rffT"
                     /locus_tag="ACHOWS_001520"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFQ0047115.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMVNDRQREVKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8652..9902)
                     /gene="wzxE"
                     /locus_tag="ACHOWS_001521"
     CDS             complement(8652..9902)
                     /gene="wzxE"
                     /locus_tag="ACHOWS_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFQ0047116.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQELVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9904..11034)
                     /gene="rffA"
                     /locus_tag="ACHOWS_001522"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9904..11034)
                     /gene="rffA"
                     /locus_tag="ACHOWS_001522"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFQ0047117.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKYNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11039..11713)
                     /gene="rffC"
                     /locus_tag="ACHOWS_001523"
                     /gene_synonym="wecD"
     CDS             complement(11039..11713)
                     /gene="rffC"
                     /locus_tag="ACHOWS_001523"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFQ0047118.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDALAPWSRV
                     QAKIAASNTDELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRSASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11691..12572)
                     /gene="rfbA"
                     /locus_tag="ACHOWS_001524"
     CDS             complement(11691..12572)
                     /gene="rfbA"
                     /locus_tag="ACHOWS_001524"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0047119.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12591..13658)
                     /gene="rffG"
                     /locus_tag="ACHOWS_001525"
     CDS             complement(12591..13658)
                     /gene="rffG"
                     /locus_tag="ACHOWS_001525"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0047120.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SVSKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13658..14920)
                     /gene="wecC"
                     /locus_tag="ACHOWS_001526"
     CDS             complement(13658..14920)
                     /gene="wecC"
                     /locus_tag="ACHOWS_001526"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFQ0047121.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14917..16047)
                     /gene="wecB"
                     /locus_tag="ACHOWS_001527"
     CDS             complement(14917..16047)
                     /gene="wecB"
                     /locus_tag="ACHOWS_001527"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFQ0047122.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16103..17149)
                     /gene="wzzE"
                     /locus_tag="ACHOWS_001528"
     CDS             complement(16103..17149)
                     /gene="wzzE"
                     /locus_tag="ACHOWS_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFQ0047123.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYFRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17161..18264)
                     /gene="wecA"
                     /locus_tag="ACHOWS_001529"
     CDS             complement(17161..18264)
                     /gene="wecA"
                     /locus_tag="ACHOWS_001529"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFQ0047124.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18504..19763)
                     /gene="rho"
                     /locus_tag="ACHOWS_001530"
     CDS             complement(18504..19763)
                     /gene="rho"
                     /locus_tag="ACHOWS_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0047125.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19848..19949)
                     /gene="rhoL"
                     /locus_tag="ACHOWS_001531"
     CDS             complement(19848..19949)
                     /gene="rhoL"
                     /locus_tag="ACHOWS_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFQ0047126.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20090..20419)
                     /gene="trxA"
                     /locus_tag="ACHOWS_001532"
     CDS             complement(20090..20419)
                     /gene="trxA"
                     /locus_tag="ACHOWS_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFQ0047127.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIAGEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20550..21815
                     /gene="rhlB"
                     /locus_tag="ACHOWS_001533"
     CDS             20550..21815
                     /gene="rhlB"
                     /locus_tag="ACHOWS_001533"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFQ0047128.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21949..23433
                     /gene="gppA"
                     /locus_tag="ACHOWS_001534"
     CDS             21949..23433
                     /gene="gppA"
                     /locus_tag="ACHOWS_001534"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFQ0047129.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23480..25501)
                     /gene="rep"
                     /locus_tag="ACHOWS_001535"
     CDS             complement(23480..25501)
                     /gene="rep"
                     /locus_tag="ACHOWS_001535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFQ0047130.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25588..25869
                     /gene="ppiC"
                     /locus_tag="ACHOWS_001536"
     CDS             25588..25869
                     /gene="ppiC"
                     /locus_tag="ACHOWS_001536"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFQ0047131.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25915..27390)
                     /gene="ilvC"
                     /locus_tag="ACHOWS_001537"
     CDS             complement(25915..27390)
                     /gene="ilvC"
                     /locus_tag="ACHOWS_001537"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFQ0047132.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETVTEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27540..28430
                     /gene="ilvY"
                     /locus_tag="ACHOWS_001538"
     CDS             27540..28430
                     /gene="ilvY"
                     /locus_tag="ACHOWS_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFQ0047133.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLSTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28427..29971)
                     /gene="ilvA"
                     /locus_tag="ACHOWS_001539"
     CDS             complement(28427..29971)
                     /gene="ilvA"
                     /locus_tag="ACHOWS_001539"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFQ0047134.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29974..31824)
                     /gene="ilvD"
                     /locus_tag="ACHOWS_001540"
     CDS             complement(29974..31824)
                     /gene="ilvD"
                     /locus_tag="ACHOWS_001540"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFQ0047135.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31889..32818)
                     /gene="ilvE"
                     /locus_tag="ACHOWS_001541"
     CDS             complement(31889..32818)
                     /gene="ilvE"
                     /locus_tag="ACHOWS_001541"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFQ0047136.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32838..33101)
                     /gene="ilvM"
                     /locus_tag="ACHOWS_001542"
     CDS             complement(32838..33101)
                     /gene="ilvM"
                     /locus_tag="ACHOWS_001542"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFQ0047137.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33098..34744)
                     /gene="ilvG"
                     /locus_tag="ACHOWS_001543"
     CDS             complement(33098..34744)
                     /gene="ilvG"
                     /locus_tag="ACHOWS_001543"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFQ0047138.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34747..34797)
                     /gene="ilvX"
                     /locus_tag="ACHOWS_001544"
     CDS             complement(34747..34797)
                     /gene="ilvX"
                     /locus_tag="ACHOWS_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFQ0047139.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34884..34982)
                     /gene="ilvL"
                     /locus_tag="ACHOWS_001545"
     CDS             complement(34884..34982)
                     /gene="ilvL"
                     /locus_tag="ACHOWS_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFQ0047140.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35335..36855
                     /gene="yifB"
                     /locus_tag="ACHOWS_001546"
     CDS             35335..36855
                     /gene="yifB"
                     /locus_tag="ACHOWS_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFQ0047141.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36881..37219)
                     /gene="maoP"
                     /locus_tag="ACHOWS_001547"
     CDS             complement(36881..37219)
                     /gene="maoP"
                     /locus_tag="ACHOWS_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFQ0047142.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37338..38177
                     /gene="hdfR"
                     /locus_tag="ACHOWS_001548"
     CDS             37338..38177
                     /gene="hdfR"
                     /locus_tag="ACHOWS_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFQ0047143.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38272..38347)
                     /locus_tag="ACHOWS_001549"
     tRNA            complement(38272..38347)
                     /locus_tag="ACHOWS_001549"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38312..38314),aa:Trp,seq:cca)
     gene            complement(38356..38432)
                     /locus_tag="ACHOWS_001550"
     tRNA            complement(38356..38432)
                     /locus_tag="ACHOWS_001550"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38396..38398),aa:Asp,seq:gtc)
BASE COUNT         9423 a        10501 c         9708 g         8839 t
ORIGIN      
        1 gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttcta gataaataaa
       61 taatttattt ttatcactat tatttatgca atcatcctgt cagggagagg gatctcaatt
      121 atcaatgctt aattacgtca tcattttgac gacatgaaac cggagcattt tgcatggaat
      181 tttcgttttc acccaaactt cttgttgtgg ctgtcgccgc cgctcttcca ctcatggcca
      241 acgcagcaga taccccgtca accgccaccg cacgcaaagg ctttgccgga tacgatcacc
      301 caaaccagta tctggttaaa ccggcgacca ctattgccga caacatgatg ccggtgatgc
      361 agcatcctgc acaagacaaa gaaacccagc agaagctggc agaacttgag aaaaaaaccg
      421 gtaagaaacc gaacgtggtt gttttcttgc tggatgatgt gggctggatg gatgttggct
      481 ttaacggcgg cggcgtggcg gtgggtaacc ctacaccaga tatcgacgcc gttgccagcc
      541 aggggctgat tttaacttcg gcgtattctc aaccgagttc ttccccaacc cgcgccacga
      601 ttcttaccgg acaatactcc atccaccacg gcattctgat gccgccaatg tacgggcaac
      661 cgggcggtct gcaagggtta accacgctgc cgcagttgct gcacgatcag ggctacgtca
      721 ctcaggccat cgggaaatgg catatggggg aaaacaaaga gtcgcagccg cagaacgttg
      781 gctttgatga tttccgcggc tttaactcgg tatccgatat gtataccgaa tggcgtgatg
      841 ttcacgtcaa cccggaagtc gccctgagcc cggatcgttc tgaatacatc caaaaactgc
      901 cgttcagcaa agatgacgtt catgccgtgc gcggcggtga acaggaagcc attgccgaca
      961 ttacgccgaa atatatggaa gacctggatc aacgctggat ggaatatggc gttaagttcc
     1021 tcgacaagat ggcgaagagc gataagcctt tcttcctcta ctacggcact cgcggctgtc
     1081 acttcgataa ctacccaaat gcgaaatatg cgggtagctc tccggcgcgc acctcttacg
     1141 gcgactgcat ggtggagatg aacgatatct tcgccaatct gtataaagca ctggagaaaa
     1201 acggtcagct tgataacacg ctgattgtgt tcacctctga taacggtccg gaagccgaag
     1261 ttccgccgca cggtcgcaca ccgttccgcg gtgcgaaagg ttctacatgg gaaggcggcg
     1321 ttcgtgtgcc aaccttcgtt tactggaaag gcatgatcca accgcgtaaa tctgacggca
     1381 ttgtcgatct ggcggatctc ttccctacgg cgctggatct ggcggggcat cctggggcga
     1441 aagtagcgaa tttagtaccg aaaaccacct ttatcgatgg tgtggaccag acatctttct
     1501 tcctgggaac aaatggtcag tctaaccgta aggccgagca ctacttcctc aacggtaaac
     1561 tttctgctgt gcgtatggat gagttcaagt atcacgtcct gattcagcaa ccttacgctt
     1621 atacccagag cggatatcag ggtggattca ccggcacagt aatgcaaacg gcgggatcgt
     1681 cggtgtttaa cctgtacacc gatccgcagg aaagcgactc tatcggcgtg cgccatattc
     1741 cgatgggtgt accgctacag accgaaatgc acgcgtatat ggagatcctg aaaaaatatc
     1801 caccacgcgc gcagattaaa tctgactaag ccggcgctat cgctgagatc tgcctttgcc
     1861 ggatgcgatg ctgacgcatc ttatccagcc tacagaacgc tgcaatttat tgaatttgca
     1921 atgattgtag gccggataag gcgttcacgc cgcatccggc aatcaaccgc aggcggccgc
     1981 cgatttctac ttattcacca ccatcaaatg cgcctgcata atgtcgctgg ccggacgacc
     2041 gtgcgccagc aaatcagcca ttgctttaag atatggcggt agatggcgga aataacgctg
     2101 atacccggca cacaaataat tcagcccggg tttgccgctg gcatcgagca taaagcggtg
     2161 tttcgggcag cctccccagc acgcttttaa cacgttgcaa ctgcgacact gtgccggtaa
     2221 ctgcttgaat ttatcttcac caaatgcctg ctgttgcggg gaatcaatca tttctgcaat
     2281 cgtctgctga agcatattcc ctagtcgata ctgcggataa acatagtgat cgcaggcgta
     2341 aacatcgccg ttgtgttcaa caatcaccga gcgcccacag gttggctgat gatggcaaac
     2401 cgcgcccggt gcgccgacaa aattggcgaa cgcccattcg atattcatta cgaaaacctt
     2461 gccgacgtcg cgcttaatcc agtggtcgaa aatcgccacc agaaactcac cgaactcttg
     2521 cgggcacacc gaccattccg tcagttcgcc ctgaatatcg ccgggagcat gtagcttaag
     2581 tccagcatga gcagccgttt catcagccag gcgctcgacc accggaataa actggatgaa
     2641 ctcaacacct gcatcacaca gaaaatcata gacttgcaaa ggttgtaacg cactggtgcg
     2701 atttacgcag accagcacgt tatagtcgac atgatgtttt tgcaggagcg tcagggcacg
     2761 catcaccagc ttatgcgttg gtctgccgcc tttggtcacg cgatattgat tgtggatctc
     2821 agccggacca tccagcgata acccaacaag aaaatgattt tctgccagaa atgcacacca
     2881 ttcgtcatca agcagcacgc cgttagtctg gaagctgtta cttatcttcc tgccagcacc
     2941 atatttcgcc tgtagcgcca cggcacggcg gtaaaaatcc agcccgagta acgttggttc
     3001 gccgccctgc caggtaaaag ccacttcgtt ttgggtttcg ctggcagcga tatagtgacg
     3061 gacatacgct tccagcgtgt catcgtccat atgcgtgact ggcttttcgc ggtaaaggga
     3121 ttgtttttcg agataaaaac agtagtcaca gttcagatta caatcggaac cgctcggttt
     3181 cgccatcaca tgaaaagcac gcgttggaac ctgttgcagc atggtcgctc cttgcctgaa
     3241 gtcatccttc aataagttaa gggcgaaatc ataccgtggc gcaatgcgtt attacgggcg
     3301 tttattgcga agggaaagtg acgaaatgca cagaaaacaa aaaaccccgc cgaagcgggg
     3361 ttcaaaattg gtcggcgaga gaggattcga acctccgacc cactggtccc aaaccagttg
     3421 cgctaccaag ctgcgctact cgccgaaata ctgctttttg aatttttagt tcaattcttt
     3481 aaagtcgtgg tgcgatgggg gggacttgaa cccccacgtc cgtaaggaca ctaacacctg
     3541 aagctagcgc gtctaccaat tccgccacct tcgcattgtc acaacttcta ataatggggt
     3601 ggctaatggg attcgaaccc acgacaactg gaatcacaat ccagggctct accaactgag
     3661 ctatagccac cactacaaat cttgttacgc ggtattacta ccaccgcagc tcaagcgccg
     3721 ggactaaatg gcgcgcccga caggattcga acctgagacc tctgcctccg gagggcagcg
     3781 ctctatccag ctgagctacg ggcgcttagc gccgttgcgg gggtggataa tacggacttc
     3841 ccacccctct gtctagtccc tttttaaata aattgcgcgt ttggttacgg tttgtgcgct
     3901 ttgctgctta tttctccagt ttatgcgctt taccgtggcg attaagccca aaaactttat
     3961 aaatcgccgt caccgtcagc ataaagatga tgccgacaaa cagcgacata cgcgtatctt
     4021 cattaaagta catgccgatc aaaacgcaaa tcaggaatgc cattgttacg tagttggccc
     4081 acgggaacag gattgagcgg aacggatggc tggcaattgc cgctttatgc gcacgacgaa
     4141 aacgcagctg gctaatcaat atcacaaacc atggcaccat ccccggaagc acgctggcac
     4201 tgtagacgta gacaaacaca cgctgcggat tgggaatgat gtagttcagg catgagccaa
     4261 ttaacaggat agcaatagat actgccacac ccgcaaccgg tacgccgtga cgggaaactt
     4321 tcgccatcgc cgtcggtaac tgacggtttt tcgccaatgc gtagagcata cgtccgcaac
     4381 tgtacatgcc gctgttacag ccagagagcg cagccgtcag caccacaaag ttgataatgc
     4441 ccgccgctgc ggtaatgccg attttggcga aagtcagtac gaacgggctg ccgttactgc
     4501 ctatttcatt ccacgggaag atggtgacga taacgaaaat cgcgcctacg tagaaaatca
     4561 ggatacgcca caacactttg cctacggcac tgcgcagcgt cacctgcgga ttcttcgctt
     4621 caccggcagt aatgccaatc agctccacgc cctggtagga cgccaccaca atacacaatg
     4681 cggtcaggaa ccctttccag ccacccgcaa agaaaccgcc atgctctgtg agattgctaa
     4741 aaccaatcga ctgcccgcca ttgccaaaac caaagaaaat cacgcccagg ccaatgacaa
     4801 tcatcacgat aatcgtggtg actttgatca tcgcgaacca gaactcgatt tcgccgtaca
     4861 accgcactgc cgccagattc gccagcgcca ccagcgccac cgcgatcaat gcgggtatcc
     4921 actgcgccat ctccgggaac cagaactgga cataaacgcc aatggcggtg atttctgaga
     4981 tccccaccgc catccacata aaccagtaag accaggcggt gagatagcca aaaaacgggc
     5041 tcatataacg atgcgcataa acggcgaacg aaccggtaac cggttcgagg aacaacattt
     5101 cgcccattga acgcatgatg aaaaagacga acagcccggc gatgatatag gccaacaata
     5161 cggatggccc ggcccatttc agggtactgg cggcccccat aaacaggccg acgccaatgg
     5221 tgccccccag ggcgatgagt tcgatatgtc gagcttccag cccacgctgt agctctggtt
     5281 tgttatctgc cataaatcct cgtgttgtgt ttgcatgctt tccggtgtta ccggttatcg
     5341 ttatgggtac atcgagtgtt gcgaatgttt tcgtaattca ggagaaatgg caaataaagc
     5401 attaaaaatt tgaatgcttt gtgtaataaa aaagcagaca ggcgacggag tgaccactcc
     5461 gtcgctttac aaagagagga aaatcagagg ttgccggtgt agtgccagcg taaataacgc
     5521 agcaaacgaa gctgacgctt aatgcggctc ggctgcgaaa gcaggcggta gagccactcc
     5581 agccccagcg tttgccagat tttcggtgcg cgttttacgt gaccggtgaa aacatcgtag
     5641 gtcccgccaa cgcccatata cagcgcatct ggatgcacca gacggcagtc gcgcatgaag
     5701 atctcctgct ttggcgatcc catcgcaacg gtgacgattt gcgctccgct ggcatgaatg
     5761 cgttcaaaca gcgcctgacg ctgctcgggt ttaaaataac catcctgact gccaacgata
     5821 ttcacattcc actggttgcg cagtttagct tcagtttgcg ccagcacttc aggtttaccg
     5881 cccacaagaa ataccggcgt cccttctttg cctgcgcgcg ccatcagctc ttcccagaga
     5941 tcggcaccgg caacgcggga aacctgcgcc tgcgggtact ttttacgtac tgaacgtaca
     6001 acgctgatgc catccgcata tttaaattcg gcagcgttaa ttagctccct gacctcagca
     6061 ttatcttcaa tggtcagcat tttttcggca ttaatggcaa ccagcgttcc ctgcttaagc
     6121 tgcccgtcag caaacagata atcgagggcg tgctgcatat cacgccaacc aatcaactgt
     6181 aagccacgca gcgtataggt cggtgccgtg gtgttgttat tcattgttat ccttcaacct
     6241 gcgtccggag cgatgatttt gtacgtttat gaatgagtcc ggcgctttca aaaagccagt
     6301 acaacagttt tgcgatcatc agacatgcgc cgaagaccac gataaaaaag actacgcgtg
     6361 agacaaacga atccagccct tcacgtgcca gcacgatcat attgaagatc gccccgaagc
     6421 agaaactgtg caatatcgca gccttatagc gattagtctc gcggttgccc agctcataca
     6481 gccagtcgaa ccatttgatg atcagaccaa ccacaatcgc cccgagcggg atgaacaacg
     6541 cgccgcccat caccaccagt gagcctataa gcgtaggcga gatcgccagc ccggagtggt
     6601 tattcagcac ttcccaggta aagtagttgg cggagttcag taccatactc gggcgacccg
     6661 gccacagcca ggaagggata aagacataga aatcgcggac aatgggagcc aggccctgga
     6721 agtcgatgtt gtcgtagttc tgcaacagca acgccagatt ctcccacggc gaaaaggtat
     6781 cgcgggtgag atagagaaac gtatagaacg cttcatcgcc gctcacattc attccataac
     6841 gttttagcgc cagccagaac atgcccacaa tccccagcac gcccgccgcc gccagcatcc
     6901 acaacgaaat ccagccgcga ataatgccaa taaacaggaa gatagcgaat gcgatgatga
     6961 tattggcgcg agtgccgccg acaatcatgt aagtcaacaa gccaaaggca accgtgctga
     7021 cgaggaaaaa cagccacgct ttgctatcct ggcgcagaaa ataaaccacc agcatcgccg
     7081 ggatgaaaaa gtaaaagaag cgttttaatg ccacgccgga gacttcgctg gaaaagatct
     7141 gactgtaaga attaagccgg aacagcaaaa agccgttgtg catgaagaag atgccgacgc
     7201 tcaccagagc gatgcccatc aggatcaccc acgtgagatt ggtctccacg cggttcatgg
     7261 taaacagcgg acggcgcggt gcatcagcaa cgcgtttgcg tagacgggtt ttgtaggtga
     7321 catagtaaac cgcgtagaag cagcctgcag aaagcaacgc ctgcaacaag atttctggcg
     7381 gcgcgacacc aacatcaaag cgaaatacca gcacgctggt cagcggaaag ccgaagaaaa
     7441 aggtgagcaa aaacagcaat gaaaagaaga cattgaagtt aaagcgcaca cggcgaaact
     7501 caaaccaggt cagcgtggca ataaacaacg tgcagagcag ccagacaaca aacaggccac
     7561 tgaattgcag cagactcatg cgacctcccc ggcggcaatc gccaacgccc gctgccagcc
     7621 ttgtagatag ttagggctaa agaaggcaat ggtgttttta tccaccgacg ccaactggcg
     7681 ctgcgcttca cgcacaatat cctcgttgag atcatcggta gtaaacagta ctggcaaatg
     7741 ctgttccgtc atatcctgcc agaacggatt ttcccggtta agcacacaag gaatgcccgc
     7801 ctgaatcagt aagcaaagcg taccaatgcc ctgctggcgg gcaaaaataa agtaaccgag
     7861 atcgcactga cgaagtagcg tcagataggc gtcaaattcc agtttttcgc tcagaacttg
     7921 cagattttct tcgctgaata actccagtcc cgcctgacga acttcctcga tgtacgcttc
     7981 gttattaggc ggatatccca tcggcaccac cacttttacc gtatcgccaa attgctgatg
     8041 aacggcgcgc aaggcagcaa tatgctcatt gctgcggtca ccggagttcc ccaccagaat
     8101 ggtcattttt acttcacgtt gccgatcgtt caccatcgta ttaagagaag agtccatccg
     8161 cgtcgggaag tacagcagtt cgccccgcac ctttgggtgc gttttggcaa aaaagctcaa
     8221 atcaccgcgg gtggcaaata cacagccgac tcgcttttgc gccaggcgac gtagtgggta
     8281 aaaaagctta tatctcaagc cactggaaag ttcgtacagg tctgcccccc agatatgcca
     8341 gtaaaactgg ctgggcttaa tgccaccact cagcagagcc agccacagtg tgggattgaa
     8401 ctgaccgtgg aagaaaaaac gctgctgacg gttagctttt gctttcgcga tgaccgcttc
     8461 cgccagcgat tttttcccag ggaaaaattg cacagaaagc gccagacagc tatcacttaa
     8521 gccgtcgtcc ttgccaacaa ccataaactc gcgcgcgtgc tcgctcgtcg cggccagcgc
     8581 gtcgttgaaa aaccgcagaa cggttcggtt atggtgaggg atatccgatc ccagtacgtg
     8641 aatcagtaca gtcatgcccg cctacgccag agtaaaaaca cgccacaaca aagagaaaaa
     8701 tagacgatat aggttgccat ataggcctgc gctgcgccca gtgcaccatg cgcagggatt
     8761 agccagtggg caaataccat caataaagtg aactggctga cttccgccag aatataaaac
     8821 cgcagtgacg ctttggcgat caccagataa ccaaagacat aagcgcccac ttttaacaca
     8881 tcacccacta actgccaggc aaagagatcg cgcatagcgg taaatttatt cgacaacagc
     8941 agccagatag caaaatcacg cagcaaccag acggtaaaac tcgccgccgc cactgccggt
     9001 aagacgaatt ttagcgattt aaccacttcc cgggtgatat cgcgcttttc cgttagccgc
     9061 gacaacgtgg gcagcaaata tacgctgaac gatgccgtaa taaattgcag gtaggcatcg
     9121 gaaatactgc tcaccccttg ccagatcccc acctcatccc agctatactg cgccgccagc
     9181 agtttacgca tcatgatgta agcaacaggc aaggtcaccg acgtaatcaa cgccatgagc
     9241 gtaaatttgc tcaactgccc tgctagaccg ttatcccagc tgggtttcag atagcttaac
     9301 gggatgacac cacgtttgat caacatgatg gcggcaggaa ttaccaccag cgcgggaatc
     9361 agcgccagac ccagcaacgc cccttcataa ccgcccaaac ggtaactgac gtagtacgca
     9421 agaacgccaa tcaagctgcc aacaatcagc gataacgcat tacctgcggc atcgcgaaag
     9481 cctttcatca acgccagtaa caggttgccc caggcgatcc ccatttgcac caacgccact
     9541 aaacgcacca gctcctgata gtcggtatta ccaaacaatc cctggctgat tggcgcagct
     9601 gccagcacaa aaaccagcgc catcagcgta gagaaaccaa gtaccatcgc tgatgaagtg
     9661 ccgaccacgc ggcgcagctg ttggggatta tcatggtact gggcaacgta tttggttacg
     9721 ccgttaaaga tgcctgcccc ggcaagcacg ccgagcacgg taatcaactg gcggaaattt
     9781 gccgccagcc caagccccgc cggaccaaat gacactgcca gcaacttacc gaccagtaac
     9841 ccggcaccaa tcttgaccag tgtactggcc gcagtccaca aggacgcttt tgccaacgac
     9901 atatcaggag aagtagttca acaaggtcgc aattaccgta cgctgattga cgggcgacag
     9961 gttgtagaac aacggcaggc gcagcaggcg ctcgctctct ttggtggtgt agcgatcttc
    10021 accgtggaac tcaccaaagc gttcccccgc agggcaaccg tgcagcggaa tgtaatggaa
    10081 caccgccatg atttccgctt ctttcagaaa gttaatcaac gcgctccggt catcaatatc
    10141 ccgcagttta atgtagaaca tatgcgcgtt ctgcacgcag ccatcgggaa tcgacggcag
    10201 ctcgatacgc ccggctttcg ccagaggtgc taacgcatcg tagtagtttt gccacagcgc
    10261 cagacgttgc tgattgatac gatccgctgc ttccagttgc gcccacaggt acgcagcttg
    10321 caaatcggac atcaaatagc tggagccgat atcgcgccag gtatatttat cgacctgacc
    10381 acggaagaac tggctgcggt tagtgccttt ttcacggatg atctcggctc gttcgatcaa
    10441 cgctttatcg ttaatcagcg tcgcaccgcc ttcaccgccc gccgtgtagt ttttggtttc
    10501 atggaagcta aagcagccaa tatgaccaat ggttcccagt gcacgccctt tgtaagtgga
    10561 cataacgccc tgagcggcat cttccaccac aaacagatta tactttttcg ccaacgccat
    10621 aatggtgtcc atttcgcagg ccacacccgc gtaatggacc ggcacgataa cgcgcgtttt
    10681 gtcggtgatc gccgcttcaa tcagcgtttc gtcgatgttc atagtgtccg ggcgaacatc
    10741 cacaaagacg attttcgcgc cacgcagcac aaaggcattg gcggtggaga caaaggtgta
    10801 gctcggcatg atcacttcat cgccaggctg gatatcgagc agcagcgccg ccatctccag
    10861 agaagcggtg caggacggcg tcaacagcac tttggcgctg ccaaaacgtt gttccagcca
    10921 ctgctggcag cgacgggtaa aaccgccatc gccacacagt ttgccgctac ccattgccga
    10981 ctgcatatag tcgagttcgg ttcccaccac cggcggtgcg ttaaatggaa tcatgtgatc
    11041 acctgtataa ccagtacgcg gtgctttcta cattcgcacc actttgtatg tatcgtttaa
    11101 gcgcggcggt gttgcccatc tgggtcgcca cccgcaaagt tgttttaccg cgagcatacg
    11161 cccagtttag cgctgtttgc atcagctcag cacctgcacc gcgtccagcc agcaggccaa
    11221 ttcgcgcatc tgtcgcattg agttcccgta aagagacata gccgcgaata tcgccggacg
    11281 ccgaacgtaa aatgaggcat tgatgatcaa aggtgccgcg cacggcattt tcaatccact
    11341 gtgcataaaa gcgaccgctg gcgtcaggcg cataccacgg cgcacgaaaa cggctttgcg
    11401 caaatgcggc gctggctaac tgacgtaatg cgggaatatc ggtctcttgt gccactacag
    11461 caccactatc actgacattg tttacgggta gcgccaaatc aacttcacct tctaccaggg
    11521 agaatcccag ctgttgcaag gcatccagtt catccgtatt tgatgccgca attttggcct
    11581 gcacccgtga ccacggcgct aaggcgtctg gcgtcaggag cggtgcttca ggactaatgc
    11641 gcacaatggc gctgttaaca ccaaagaagg cgttttccca ggttagtggc tcaatactgg
    11701 cgcggacggg cacgaagtaa ctccagcaga tattggccgt agccagtttt cgccagcgag
    11761 ctggcggcgc gcttcacgcc ttcgtcatca agccagccgt tacgccaggc gatctcttcc
    11821 aggcaggcaa tcttaaagcc ctggcgtttt tccaccgtct gcacaaaggt gctggcttca
    11881 atcaggctgt cgtgagtgcc agtgtccagc caggcaaatc cgcgcccgag cagttcaacg
    11941 gtcaggttgc ccgcctcgag gtacatctgg ttgatggagg taatctccag ttcaccacgc
    12001 tccgacggct tcacctgctt tgcgtactcc acgactttac tgtcgtagaa ataaagcccg
    12061 gtcaccgccc agtttgactt cggctgtttt ggcttttctt ccagcgagat agcgcggaaa
    12121 ttgtcgtcaa actccacgac gccaaagcgt tccggatcca tcacctgata gccaaatacc
    12181 gtcgcgcctt gcgtgcgcgc cgcaacatga cgcagcttcg gactgaagcc ctgtccgaag
    12241 aagatgttat cgcccagcac cagacaagaa ggttcaccgt tgaggaaggt ttcaccgatg
    12301 ataaaggcct gcgccagacc gtccggactg ggctgttcgg catattccag ctggataccg
    12361 aactcactac catcgcccag caggcgctgg aaataacctt tatcttccgg cgtagtgatg
    12421 atgagaattt cgcggatacc ggccagcatc agcaccgaca gcggatagta aatcattggc
    12481 ttatcgtaaa tcggcaacag ttgcttcgat acgccgcgcg taatcggatg caatcgggtg
    12541 ccggaaccgc ccgccaggat aatacctttc atgtttgcct ccgctgaaaa ttagcctttc
    12601 agacctaaac gctcgccctg atagctgccg tcctgcacct gcttccacca gctttcatta
    12661 gccagatacc actgaaccgt tttacgcatt ccactttcaa aggtttcctg cggcagccag
    12721 ccaagttcac gggcaatttt cgaagcatca atggcataac gcagatcatg ccccggacgg
    12781 tcagcgacaa aggtgatcag gtcacgatag tgcgccacgc cgtgcggctt gttcggagcc
    12841 agttcttcca gcagctcgca aatggtttcc acaacatcga gattcttacg ctcgttgtga
    12901 ccaccaatat tataggtttc accgactttc ccggtggtcg ccacgcaata cagcgcgcgg
    12961 gcgtgatctt ccacatacag ccagtcacgg atttgctgcc cgttgccata taccggcagc
    13021 ggtttacccg ccagcgcgtt gaggatcatc agcgggatca gtttttccgg aaagtggtaa
    13081 gggccgtagt tattcgagca gttggtgata agcgtcggca gaccataggt ccgcagccag
    13141 gcgcgcacca gatggtcgct gctggctttt gacacggaat aagggctgct cggcgcatac
    13201 ggcgtggttt cggtgaagaa atcatccgtc gagtgcaggt caccatatac ttcgtcggta
    13261 gagatatgat gaaaacggaa cgctgatttt ttatcttccg tcagcgcatt ccagtaagcc
    13321 cgcgccgctt caagcaatgt ataagtcccg acaatgttgg tttcaacaaa cgctgccggg
    13381 ccgtcaatag aacggtcaac atggctttcg gctgccagat gcatgacaca gtctggctga
    13441 tgctcagtga atacgcgcgc cagttctgcc cgatcgcaga tatcaacttt ctcaaaggca
    13501 aagcgctcgc tttgcgcgac cggtgccagc gacatcagat ttccggcgta ggtcagctta
    13561 tcgaccacta ccaccgcgtc gctcgtttcg ttgatgatat aacgcaccag cgccgagcca
    13621 ataaacccgg caccacctgt tatcagaatt tttctcatca gcgccagact cctttggcat
    13681 cgacgacata ctgctgatgg acattgtcgc cattgataac tttgaactga ctatgatcga
    13741 ccagcatcac cagcacgtct gccgttgcca gcgcctcgtc aagctgcgcc agtgtacaaa
    13801 gcccggtcag ttttttcggc aactggtgga tgttaggctc aacaaccaga gtttcgccgc
    13861 tatgccactg ggcgatcagt tcagcgattt ccatcgctgg gctttcgcgc aggtcatcaa
    13921 tattcggttt aaacgccaga ccaaagcagg cgattttcag ttcactggcg cgtttatcgg
    13981 tagcagcaag gcagtcggca accgccgctt tcacctgatc gataacccag aacggtttgt
    14041 gatcgttcac ttcgcgcgcg gtacggataa gccgcgcctg ctgcgggttc tgtgccacga
    14101 taaaccacgg atcaacagca atgcagtgac cgcccacgcc aggaccaggc tgaagaatat
    14161 taacgcgagg gtgacgattc gccaggcgaa tcagttccca gacgttaatt ccctgatcgg
    14221 cacaaatcag cgacagttcg ttagcaaaag cgatattcac atcgcggaag ctgttttcgg
    14281 tgagcttaca catttccgcc gtccgcgagt tagtgacgac acactcacct tcgaggaaaa
    14341 ttttgtacag ttcgctggcg cgggccgaac aaaccggcgt cataccacca atcacgcgat
    14401 cgtttttaat cagctcgacc ataacctggc ccggtaacac gcgttccggg cagtaagcaa
    14461 tgttgacgtc cgccaactcg cccacctgct gtgggaaagt gagatctgga cgcatctctg
    14521 ctaaccattc tgccatcttc tcggttgacc ccaccggcga ggtggattca aggatcacca
    14581 gcgcgccttt tttcagcact ggcgcaatgg agcgagcagc cgattcaacg taggtcatat
    14641 ctggctcatg atcgccttta aacggcgtgg gtacagcaat cagccaggca tccgcttcaa
    14701 ctggcgtcgt gctcgctcgt aaaaaaccgc cttctacggc agtttttact acgctcgcca
    14761 aatcaggttc gacgatatgg atttcgccac gattgatggt atcaaccgcg tgttggttga
    14821 tatcgacacc aatcacctgt ttttgccgtg aggcaaacgc agctgctgta ggcagcccga
    14881 tgtaacccag tccgataaca gaaatggtcg caaaactcat agtgatatcc gattattttt
    14941 taacgcttcc agaatgcgag agcatgcctg accatcacca tacgggttat gggcgcggct
    15001 catagcttga tattcgtttt cgtcttttaa aagacgcgtc acttcctcga caattcgctg
    15061 cttatccgtg cctaccagac gcaccgtacc cgccgtcacc gcttccggac gctcagtggt
    15121 atcgcgcatt accagcaccg gtttccccag cgaaggcgct tcttcctgaa tgccgcctga
    15181 gtcggtcaaa atcagccagg cgtggttcat cagccagaca aacggtaaat actcctgggg
    15241 atcgatcaga atgacatttt tcacgtgccc cagaatgcga ttgaccggct ctctgacgtt
    15301 cgggttgaga tgcactggat agacaatctg gatgtcctgg tgcgtggtgg cgatgtctgc
    15361 cagcgcatgg cagatttctt caaagccacg accgaagctc tcacgcctgt gaccggtcac
    15421 cagaatcatt tttttattgg ggtcgataaa cgggtaattt gccgccagtt ctgaacgcag
    15481 cgtgtcgctg ctcatcacct ggtcacgcac ccataacagt gcatcaatga ctgtattacc
    15541 agtaatgaag attcggctat ccgcaacgtt ttcacgcagc aagttttgcc gggaagtttc
    15601 ggttggagag aagtgataca tcgccagatg cccggtcaat gtacggttag cctcttccgg
    15661 ccacggcgaa tagagatcgc ccgtgcgcag accagcctca acgtgaccaa caggaatacg
    15721 ctgataaaac gccgccaggc tggttgccag cgtcgtcgta gtatcgccgt gaaccagcac
    15781 gacgtctggt ttgaactcgg caagaatagg ttttagccct tccagaatcc gacaggttat
    15841 ctctgtaagg ccctgtcctg gctgcattat gttgagatcg tagtcaggta caatggaaaa
    15901 gagtttcagc acctgatcga gcatctcccg atgctgcgca gtgacgcaaa ctttagcctc
    15961 aaaaaaagga tcttttgcca acgcatgcac caacggcgcc atcttgatgg cttccgggcg
    16021 cgtaccaaat acagtcagta ctttcacatc gattctcttc gaataagcgg cgagcgcctt
    16081 tgcgctcacc gcagcagtgt tgctatttcg agcaacggcg ggttaatgcg acaccagccc
    16141 cgatcagtcc cccgacaatg ccccacataa tcatcaggaa ggcacgacgt gggctatcgc
    16201 gttttaccgg ttcttccggc gtacgaaaat agcgataggt ctgaaaacgc ggatccaggg
    16261 ttggaccaac attcagggtg tttaacatgg cccgattctg atcatagtcg agatcaaagg
    16321 ccggaccgac ggcctgtaaa ttttccagtc gagcctggag cattggacgc ccaagcagga
    16381 acatttctga atcaggtaat tcctcggcag gcacatctgt cgcactgcgc gaaatattat
    16441 gctgctcagc aattttcagc gcctgttcaa tactgttcat ccggcgatcg tagatggctt
    16501 tcgccacctc ttcctgacgc ttcacctgag ctttcatctg gatggtgcgt gccgcccatg
    16561 cgcctttcag ctcatcattc agatggctgg ctgcacgctg gctggcaaaa gcaacatact
    16621 gacgtaacag gttattagcg tcaggcgcag tttcggcaat cagcttcacg ctgtcattga
    16681 ccgcgcgggt gaagtcaccg ggaataaaca cgatgttgtt gatcatttca tccagcaacg
    16741 ccgcatcggc tttgctgttg cccaccatcc gctgtttgta atagtcggtt tgcagccaga
    16801 actctctgcg ggtatcccac gaggccagtt gcataacaaa ctctttataa gcttcgtcca
    16861 tgaccgatgg ttggtcggca gaagccatgt ttgaacggac atccaggtta cgcaaaaatt
    16921 gctgctgtga gtaatatccc cccagcatat tcaccgttgg acgatcggta atcgctgtcg
    16981 agctccactc ctggcgagca aaaaaagtat acgccagcgc gattaacgca aacgccagcc
    17041 ccatgccaat aatccacagc ttcccagccc acaaggtacg aaacaaccca cgaatatcca
    17101 gttcattttc agcgtcttcg gccggtttcc caggcattgg ttgtgtcatc acatcctcat
    17161 ttatttggtt aaattggggc tgccaccacg atttctacgc agtctgcgtt ttacgcgctt
    17221 aataaagcga gcaactttcc aggcacgctt gatgcaatat ccatagagga agaatgctag
    17281 caaaaagagc accagcatga cccactccgg gacaaaatga gaatattctg ccagcacgcc
    17341 aatggaagcg agcagtgctg cggcaagggt aatcagcaca aacgcctggc gggaagtaaa
    17401 cccggcacgc atgatcaaat ggtgaatatg ctgacggtct ggagagaatg ggctcatgcc
    17461 tttacgcagg cgacggtaca taatcgccac catatccatt agcggaatgg cgattatcca
    17521 caaagcggta accgggctga tgggatgagt tttgccctgg gtcgtttcga gcaggatcca
    17581 gataacggta aaaccaatca gcgtactgcc cgcatccccc ataaagactt tgtagcggcg
    17641 acccaggata ccaaggttaa gcatgatgta tggcaggatg gcggcgatca tcgcaaagca
    17701 ccagattgcg agactggttt gcccgtcgaa ccacaaaatc ataccgattg ctgcaaacga
    17761 gacgcaggac aacccaccca gcaagccatc aatgccatca accatgttga acgcattaat
    17821 ggccgcccag acggcaaata gcgtcaggaa gtaaccaaac ggtccgagca ccatctccca
    17881 ggagccaaag atataaccta ggctactgag ataaagtttg ccgaacacca tcataacaat
    17941 gccaacagcg gcctgtatgg tggcacggat ttttacgctg atatcaaaac ggtcatccag
    18001 cgcgccaatg aaaacaagca caccagcaca agcgagatag agagatgcat gcggaatata
    18061 gtaatcgaca attccgaacg tgaagcaaat ccctgcgtaa accgaaatcc ccccaacgag
    18121 aggtatcaat ccctggtgac gtttgcggaa gttaggttta tccactaaac cgactttttt
    18181 tgccacctta cgggcaaaaa acagaaacag tgtcgtgaat aaaaaaatac tgatgagatc
    18241 agtactcact gtcagtaaat tcacaatgca tgctctcaga gaaaattatt agcagaagta
    18301 taaccacgaa gacctttatt cagaagggaa agtccgttcc gaacccacca gtcctgtgca
    18361 aaaattaacc atttgatatg caaattgcat aacacaccag caaaatccag gaattttcct
    18421 aacattgcca gcgcggcatt aagattacag atataaaagc aaaacgccac gtaaacacgt
    18481 ggcgtttttg gcataagaca aatttatgag cgtttcatca tttcgaagaa atcgtcattg
    18541 gtcttggtca ttgccagttt attaatgagg aattccattg catcgatttc gcccatcggg
    18601 tgaatgattt tgcgcaggat ccacattttc tgcagttctt cctgagtagt gagcagctct
    18661 tctttacggg taccggaacg gttgtagtcg attgccggga agacgcgttt ttcagcgatc
    18721 ttacgagaga ggtgcagttc catgttgcct gtacctttaa actcttcgta gataacttcg
    18781 tccatcttgg aaccggtatc gataagcgcg gtagcgatga tggtcaggct gccgccctct
    18841 tccacgttac gcgccgcacc gaagaagcgt ttcggacgat gcagggcgtt ggcatccaca
    18901 ccaccggtca gcactttacc tgacgccgga acaacggtgt tgtaagcgcg cgccagacga
    18961 gtgatggagt cgagcaggat gataacgtct ttcttgtgct caacaaggcg tttcgccttc
    19021 tcgatcacca tttccgcgac ctgaacgtgg cgagatgctg gttcgtcaaa ggtagaagca
    19081 acaacttcac cttttaccag acgctgcatc tcggttactt cttccggacg ttcgtcgatc
    19141 agcagaacca tcagcacaca atccgggtgg ttgtaagcaa tgctctgagc aatgttctgc
    19201 agcagcatgg ttttaccggc tttcggcggt gccacaatca gaccacgctg accacgaccg
    19261 ataggtgatg ccagatccag tacgcgagcg gttaaatctt cagtagaacc gttaccacgt
    19321 tccatacgca gacgagagtt tgcgtgcagc ggggttaagt tctcaaagag gattttgttg
    19381 cgggcgtttt caggtttgtc gaagttaact tcgttaactt tcagcagcgc aaaatagcgt
    19441 tcaccttctt tcggcgggcg aatcttacca gagatggtat caccagtgcg gaggttgaaa
    19501 cggcggattt ggctagggga aacgtagatg tcatcaggac cggcgaggta ggagctgtct
    19561 gcggaacgga ggaaaccaaa tccatcctgc aatatctcca gtacaccatc accaaagata
    19621 tcttcgccac tctttgcgtg ctgcttcagg atggcaaaaa taatgtcctg cttacgcata
    19681 cgagccaggt tttccagccc catattttcg ccgagagtga tcagctcaga aaccggcgta
    19741 ttctttaatt cggtaagatt catagtggtg tgagttctta aacttggggt aaatctcgaa
    19801 cttaatgttg tgaatggtat ggcaggatca tccatgcctg tttacggtca tcgactcatg
    19861 tcttttcgct gcctggtcac aggagagtac gcagaactga aacgacaaga cggattgagt
    19921 gacgagccag aaatctgttc acttcgcatt taagataaaa cgggaagcgt tggaaattac
    19981 aagattcaaa cttaataagg tatgtttaat acgaagtcaa cactaagtta gcatgactca
    20041 cgccgggcgt ccagttttta gcgacggggc acccgaacat gaaattccct tacgccaggt
    20101 tagcgtcgag gaactctttc aactgacctt tagacagtgc gcccactttg gttgccgcca
    20161 cttcaccgtt tttgaacagc agcaaagtcg ggataccacg gatgccatat ttcggcgcag
    20221 tgccagggtt ttgatcgatg ttcagttttg caacggtcag tttgccctga tattcgccag
    20281 cgatttcatc cagaatcggg gcgatcattt tgcacggacc gcaccactct gcccagaaat
    20341 cgacgaggat cgccccgtcc gctttgagta catccgtgtc aaaactgtcg tcagtcaggt
    20401 gaataatttt atcgctcata tataactcca caggaataag cctggcgtgt tggtttcgtt
    20461 gttggtgtaa cattaaccaa ctaaaggttg actttatttc accggatacg ctttcgtaaa
    20521 gcaatagtaa gctgatattc taccacacta tgagcaaaac acatttaaca gaacagaagt
    20581 tttccgactt cgccctgcat ccgaaggttg tagaagccct tgaaaaaaaa gggtttcata
    20641 actgtacgcc cattcaggca ctggcccttc cgctgacgct ggcgggtcgt gacgtagccg
    20701 ggcaggcgca aaccggtacc gggaaaacga tggcgtttct tacgtcaacg tttcattatc
    20761 ttctctctca tcccgcgatt gccgatcgca aggtgaatca accgcgtgcc ttaattatgg
    20821 caccgacgcg tgaacttgcc gtgcagatcc acgccgacgc ggagccactt gcgcaagcta
    20881 ccggcctgaa gctgggtctg gcttacggtg gtgatggcta cgacaaacag ctgaaagtgc
    20941 tggaaagcgg cgttgacatt ctgattggca ccacggggcg tttaattgac tacgccaagc
    21001 agaaccacat taacctcggt gccattcagg tagtggtact ggacgaagcc gatcgtatgt
    21061 acgatctggg ctttattaaa gatatccgct ggctgttccg ccgtatgccg cctgcaaacc
    21121 agcgcctcaa catgctgttc tccgccacgc tttcgtaccg ggtacgtgaa ctggcgttcg
    21181 agcagatgaa caatgccgaa tatattgaag tggaaccgga acagaaaacg ggccaccgta
    21241 ttaaagaaga gcttttctac ccttctaacg aagaaaaaat gcgtttgctg caaacgctga
    21301 tcgaagaaga gtggccagac agagcgatta ttttcgccaa caccaaacac cgttgtgaag
    21361 agatctgggg ccacctggcg gcagatggtc atcgtgtcgg tttgttgaca ggcgatgtcg
    21421 cgcagaaaaa acgtctgcgt attcttgatg aatttacccg tggtgatttg gatattctgg
    21481 ttgccaccga tgttgccgcg cgtggcctgc atatcccggc tgtgacgcac gtctttaact
    21541 acgatttacc cgatgactgt gaagattacg ttcaccgtat tggtcgtaca ggtcgcgctg
    21601 gcgcaagtgg tcattccatc agcctggcat gtgaagagta tgcattgaat ttgcctgcta
    21661 ttgagaccta tattggtcac tcaattccgg taagcaaata caatccggac gcattgatga
    21721 ccgatctgcc aaaaccgctg cgcctcacgc gcccgcgtac aggcaatggt ccgcgtcgta
    21781 ctggtgctcc gcgtaatcgt cgtcgttcag gttaaataaa atgtcatact caaaatcatt
    21841 caaactgtat caagatggca agagaatgaa tcccgatgcg catgctgtag taagtgattt
    21901 gggtgaaaga acgcagccaa cacagagaca gattgaagga tgaagagtat gggttccacc
    21961 tcgtcgctgt atgcagccat tgatctcggt tcgaatagtt ttcatatgct ggttgtgcgc
    22021 gaggtggctg gaagcatcca gacgctgacg cgaattaaac gcaaagtgcg tctggctgct
    22081 ggcctgaata gcgaaaatgc cctgtctaat gaagcaatgg agcgcggttg gcaatgtctg
    22141 cgcctgtttg ctgaacgtct gcaagatatc cctccctcgc aaattcgcgt tgtcgctacg
    22201 gcgacattac gactagccgt caatgcgggt gattttatcg ccaaagcaca ggaaatcctt
    22261 ggctgcccgg tacaagtcat cagcggtgaa gaagaagccc gcctgattta tcagggggtg
    22321 gcccacacaa caggcggtgc cgatcagcgc ctggtggtgg atataggcgg tgccagtact
    22381 gaactggtaa ccggcacggg tgcacaaacc acctcgttgt tcagcctgtc gatgggctgc
    22441 gtcacctggc tggaacgcta ttttgccgat cgtaatctgg cgcaggaaaa ttttgatgct
    22501 gcagaaaaag cggcacgcga agtgttacgt ccggttgcag atgaattacg gtatcacggc
    22561 tggaaagtgt gcgttggcgc ttccggcacc gtgcaggcgt tacaggaaat catgatggca
    22621 caggggatgg atgaacgcat taccctggaa aagttgcagc aactgaaaca acgcgccatt
    22681 cattgcggtc ggctggaaga gctagagatt gacgggctga cgctggaacg tgcgttagtg
    22741 ttcccgagtg gtctggcgat cctgatagcc atttttaccg aactgaatat tcagtgtatg
    22801 accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg gtatgttgca tcttaccgtc
    22861 gagcaggata ttcgcagccg tacgctgcgt aatattcagc gccgctttat gatcgatatt
    22921 gatcaggcac agcgcgtagc caaagttgcg gctaacttct acgatcaggt ggaaaatgaa
    22981 tggcatcttg aagcaataag ccgcgatttg ctcatcagcg cctgtcagct tcatgaaatc
    23041 ggcctgagcg ttgacttcaa acaagcgccg caacacgctg cttatctggt acgtaatctg
    23101 gatcttcccg gttttacccc cgcacagaaa aaactgctgg cgacgctact gctcaaccag
    23161 actaatccgg tcgatctctc atcgctgcat cagcaaaatg ccgtaccacc gcgcgtcgca
    23221 gaacaactct gccgtttact ccgcctggct atcatttttg ccagccgtcg ccgtgacgat
    23281 ctcgtgccag agatgacatt acaggctaac catgaactgt tgaccttgac gcttccgcaa
    23341 ggttggctaa cccaacatcc gctgggtaaa gagattattg atcaggaaag ccagtggcag
    23401 agctatgtcc actggccgct ggaagtgcat taatgagtaa gtgccggatg cgatgctgac
    23461 acatcttttc cggccttgat tatttccctc gttttgccgc catcatcgct ttcagattcg
    23521 ccagatggct ttgccctttc tgcatccgtt cttcggcgct gaccactttg cgctcctgtt
    23581 cccaaatcaa atcatcctgc ggcagctcca gcaggaagcg gctcggctcc gggcgcacca
    23641 gttctccgta ctgacggcgt tctttacaca gcgtaaaggt caattccttc tgggcgcggg
    23701 taatgccgac ataggccagc cgccgctcct catcgatatt atcttcatcg atgctgctct
    23761 ggtgcggcaa aaacccctct tccataccga ccatgtagac ataaggaaac tccagccctt
    23821 tcgacgcgtg gagagtcatc agttgcacct gatccagctc ttcttcactc tcaccacgct
    23881 ccatcatgtc gcgcaaagta aagcgcgtca ccacctgggt gagcgtcatc ggctcatcca
    23941 gttcgctgcc ttccagcatc tccgtcatcc agctaaacag ttggttgacg ttcttcatgc
    24001 gcatttcggc ggctttcggg ctgggcgatg tttcgtacag ccaggattca taatccatgc
    24061 cgtggatcag atcacgcacc gcggcaatcg gctcccgctc cgccagacgc tggatttctg
    24121 ccaaccagtg agtgaagcgg gtcaatgctt cataaccacg tccgctaagc gtctgactca
    24181 ggcccatatc aaagctggcg gtaaacatgc ttttattgcg cgtcatcgcc cattcaccca
    24241 actttttcag cgtcgccggg ccaatctctc gcttcggcgt gttaacgata cgcaggaacg
    24301 cactatcatc gtccgggtta gtcagcacgc gcagataagc cagcaagtcc ttgatttcag
    24361 gacgagagaa aaacgacgta ccaccagaaa ttttgtacgg gatgcggttt tgcatcagga
    24421 atttttcaaa cacccgcgac tgatggttac cgcgataaag aatggcgtaa tctttgtact
    24481 gcgttttatt gacgaagtga tgggcaatca gctcgccagt aacgcgctcg gcttcatgtt
    24541 cctcgttatt cgcgcttaaa acttttagct ctgtgccgta acccagttcg gagaacagac
    24601 gcttttcaaa gacgtgcggg ttattggcga tcaggatgtt cgccgctttc aaaatacgtc
    24661 cggaagagcg gtagttctgc tcaagcttaa tcaccttcag cgccggaaaa tcctgactca
    24721 gcagcaccag gttttgcgga cgtgcgccgc gccaggagta gatcgactgg tcatcgtcac
    24781 ccaccacggt aaagcgcgcg cggctgccca ccagcagttt caccagctca tactggctgg
    24841 tgttggtgtc ctgatattcg tccaccagca ggtagcgaat tttgttctgc cagcgctcgc
    24901 ggacttcttc attgcgttgc aacagcaacg ttggcaataa aatcagatca tcgaaatcga
    24961 gaacgttaca ggctttcagg tgtgcatcat acagcccata acaatgggca aaaatacggt
    25021 cccgctcgcc ctttgcctct gccgccgcct gcgccggtgt tttgagatcg ttcttccagt
    25081 tggagatggt cgaaattagc tgttgcagga gaactttgtc atcttcaatc agcccctcgg
    25141 tcaactcttt aagcaaagca agctgatcgg tatcgtcaaa cagcgaaaag ttagccttca
    25201 tccccaacgc cgcgtactca cgcttaatga tatccagccc cagcgtgtga aaggtggaga
    25261 tcatcagccc acgcgcctct ttacgcccca gcgtctgccc gacacgctct ttcatctcgc
    25321 gtgccgcctt attggtaaag gtcaccgccg cgatatgtcg cgcctgataa ccgcaaccgc
    25381 ggatcagatg ggcgatttta ttggtgataa cacgagtttt accggaaccc gcgcccgcca
    25441 gcaccaggca ggggccggta acgaattcga cagcttgttg ttggccgggg tttagacgca
    25501 taggaaaatc gctcaatgaa agtcgggagg ggaaaataac agcgtggtag tatagccagc
    25561 ctcatcctta ccactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
    25621 gtaaaagaag agaaactggc tctggatctt ctcgagcaga tcaagaatgg tgccgatttc
    25681 ggcaagctgg cgaagaaaca ctccatttgc ccgtcaggca aacgcggcgg tgatttgggt
    25741 gaattccgcc agggtcaaat ggttccggcg ttcgataaag tggttttctc ttgtccggta
    25801 ctggagccaa ccggcccgct gcatacccag ttcggttacc acatcattaa ggtgctgtac
    25861 cgtaactaac agcaaggcct tctccgggag aaggccttga actctgaatc gctattaacc
    25921 cgcaacagca atacgtttca tatccgtcat atagccgcgc agtttcttac ctacctgctc
    25981 aatcgcatgg ctgcgaatcg cttcatttac atcacgcagc tgcgcgttat ctaccgcacc
    26041 ttccggaata gctttaccca ggtcgcccgg ttgcagctct gccataaacg gtttcagcag
    26101 cggcacacaa gcgtaagaga acagatagtt accgtactcg gcagtatcgg agataaccac
    26161 gttcatttcg tacagacgct tacgggcgat ggtgttggca atcagcggca attcgtgcag
    26221 tgattcatag taagcagatt cttcgatgat gccggaatca accatggttt caaacgccaa
    26281 ctcaacgcct gcttttacca tcgcgatcat cagtacgcct ttatcgaagt actcctgttc
    26341 gccgattttg ccttcatact gcggcgcggt ttcgaatgcg gttttgccag tctcttcacg
    26401 ccaggtcagc agtttcttat cgtcgttggc ccagtcagcc atcatgccgg aggagaattc
    26461 gccggagatg atgtcgtcca tatgtttctg gaacagcggc gccatgatct ctttcagttg
    26521 ctcagagagc gcgtaagcac gcagtttcgc cgggttagaa agacggtcca tcatcagggt
    26581 gatgccgccc tgtttcagcg cttcggtgac ggtttcccaa ccgaactgaa tcagtttttc
    26641 tgcgtatgcc ggatcggtgc cttcttccac cagcttgtcg aagcacagca gagaaccagc
    26701 ttgcaacata ccgcacagga tggtttgctc gcccatcagg tcagatttca cttccgcaac
    26761 gaaagaggat tccagcacgc ccgcacggtg accgccggtt gcagccgccc atgctttagc
    26821 gatcgccatg ccttcgcctt tcggatcgtt ttccgggtga acggcaatca gcgtcggtac
    26881 gccgaatcca cgtttgtact cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat
    26941 tacgacggtg atgtctttac ggatctgctc gcccacttcg acgatgttga aaccgtgtga
    27001 gtaacccagc gccgcgccgt ctttcatcag tggctgtacg gtgcgtacta catcagagtg
    27061 ctgcttgtcc ggcgtcaggt taaccaccag atccgcctgc gggatcagtt cttcgtaagt
    27121 acccacttta aaaccatttt cggttgcttt acgccaggat gcgcgcttct cggcaatcgc
    27181 ttctttacgc agagcgtaag aaatatccag accagaatca cgcatgttca ggccctggtt
    27241 cagcccctgt gcgccacagc cgacgatgac tactttttta ccctgaaggt agctcgcgcc
    27301 atcggcgaat tcatcgcgcc ccataaagcg acatttgccc agctgtgcca gctgctggcg
    27361 cagattcagt gtattgaagt agttagccat ggtgattcct cgtgatgttg tgcttcttat
    27421 tgttcggttc gcgtttgcga gattgaattc actatatgac aggaaattta ttgcggaaat
    27481 tgatatattc acaacgtcac attgcaattt ctgcaacgtc aacattgagg gctgcgccat
    27541 tggatttacg cgatctgaaa accttcctgc atctggcgga aagccgccat tttggccgta
    27601 gcgcgcgggc aatgcacgtt agcccgtcta cgctttcgcg acaaatacag cgcctggaag
    27661 aagatctcgg tcagccgctg tttgtgcgtg ataaccgcac ggtaacgctg acggaagcgg
    27721 gcgaagagct gcgcgttttc gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca
    27781 ccatcgacca gcaagggccg tcgctctctg gcgaattaca tatcttctgc tcggtgaccg
    27841 ctgcctacag ccatctgccg ccgattctgg atcgcttccg cgcggaacat ccgtcagtgg
    27901 agatcaaact ctctaccggt gatgcggccg atgcgatgga aaaggtggtc actggcgaag
    27961 cggatctggc gattgccggg aaaccggaaa ccttgccggg cgcagtggcg ttttcgatgc
    28021 tggagaatct ggcggtggtg ctgattgctc ccgcgctgcc ctgcccggtg cgtaatcagg
    28081 tatctgtcga aaagcccgac tggtctacag tgccgtttat catggccgat cagggaccgg
    28141 tacgtcgccg cattgaactg tggtttcggc gtaacaaaat cagcaaccca atgatttacg
    28201 ccacggttgg cgggcatgaa gcgatggtat cgatggtggc gctcggctgt ggcgtggcgc
    28261 tgttgccgga agtggtgctg gataacagcc cggaaccggt gcgtaaccgc gtgatgattt
    28321 tagagcgcag tgatgaaaaa acgccgtttg agctgggcgt gtgcgtacaa aaaaagcggc
    28381 ttcatgagcc gctgattgag gcgttctgga agattttgcc gaaccactaa cccgccaaaa
    28441 agaacctgaa cgccgggtta ttggtttcgt cgtggcaatc gtagcccagt tcattcagcc
    28501 gggtttcaaa atccggttca tgatcgccaa gctcgaacgc cgccagtacg cgcccgtagt
    28561 cggtaccgtg gctgcgataa tggaacagcg agatgttcca gtgcgtaccc agcgtgttga
    28621 ggaagcgcag cagcgcgccc ggcgattccg ggaattcgaa gctgtagagg cgttcctgca
    28681 acggatgcga tggacgcccg ccaaccatat agcgcacatg cagcttcgcc atttcgtcgt
    28741 cggagagatc caccacgctg tagccgccat cgttgagcat ctgcaaaatt tctttgcgct
    28801 cttcgaggcc acggctcagg cgcacgccga caaaaatgca ggcgtttttg gcatcagcaa
    28861 agcggtagtt gaactcggtg accgagcgcc caccaagtaa ctggcagaat ttgaggaagc
    28921 tgcccttttc ttccggaatg gtcaccgcca gtaacgcttc acgctgttcg cccagttcgc
    28981 agcgttccga gacgtagcgc agaccgtgaa agttcacgtt ggcaccggaa agaatatgcg
    29041 ccagccgctc accgcgaatg ttgtgctggg cgatgtattt tttcatccct gccagcgcca
    29101 gcgcgccaga gggttcagcc accgcgcgta catcttcgaa caggtctttc atcgccgcac
    29161 agatggcatc gctatcgacg gtgatgatgt cgtcaagata ctcctggcac aaacggaagg
    29221 tttcatcgcc gatgcgtttt accgcgacgc cttcagcaaa cagccccacg cggggcagat
    29281 caacgggatg acctgcatca agtgccgctt tcaggcaggc ggaatcttcc gcttctacgg
    29341 cgatcacttt gatttgtggc atcagttgtt tgatcagcac cgccacgccc gccgccagac
    29401 cgccgccgcc gaccggaaca aatacgcggt cgagatgagc atcctgctgg agcagttcca
    29461 gcgccagcgt accctgacca gcgatcacca tcggatgatc gaagggtggc acccaggtga
    29521 agccctgctg ctgtgacagt tcgattgctt tcgctttcgc ttcgtcaaag ttcgcgccat
    29581 gaagcagcac ttcgccgcca aagccgcgca ccgcatcaac tttgatatcg gcggtggcgg
    29641 ttggcatgac gatcagcgcc ttcacgccta accgtgcgga agaaaacgcg acgccctgcg
    29701 cgtggttacc cgcagaagcg gtaatcacgc cgtgcgcttt ttgttcttcc gtcaggcccg
    29761 ccatcatggc gtatgcacca cgaagcttaa agctgtgcac tggctggcga tcttcgcgct
    29821 tcaccagaat cacgttatca agacgcgacg acagtttttc cattttctgt agcggcgtga
    29881 cctgcgccgc ttcgtagacc ggtgcgcgca gcaccgctct taaatattcg gcaccttccg
    29941 gggcaccgga caggggttgt gagtctgcca tcattaaccc cccagtttcg atttatcgcg
    30001 caccgcgcct ttgtcggcgc tggttgccag gctggcgtag gcgcgcaggg caaaggagac
    30061 ctgacgttca cggtttttcg gcgtccaggc tttgtcaccc cgagcttcct gcgcttcacg
    30121 acgcgccgcc agttcggcat cgcttacctg taactgaata ccacggttcg gaatgtcgat
    30181 ggcgatcagg tcgccgtctt caatcaggcc aatgctgccg ccgcttgccg cttccggtga
    30241 gacgtggccg atagaaaggc cagaggtacc gccagagaaa cgaccgtcgg tgatcagcgc
    30301 acaggcttta ccgagaccca ttgatttcag gaagctggtt gggtagagca tttcctgcat
    30361 ccccggaccg cctttcgggc cttcatagcg aattactacc acatctccgg cgacaacttt
    30421 accgccgaga atcgcttcta ccgcatcgtc ctggctttcg tacactttcg ccgggccggt
    30481 gaatttgagg atgctgtcat ccacgcccgc ggttttaacg atgcagccgt tttccgcaaa
    30541 gttaccgtag agcaccgcca ggccgccgtc tttgctgtag gcgtgttcca gcgagcggat
    30601 acagccattg gcgcgatcgt cgtccagcgt atcccaacgg caatcttgcg agaatgcctg
    30661 ggtggtacgg atacccgcag ggccggcgcg gaacatattt tttaccgcgt catcctgggt
    30721 cagcataacg tcgtattgtt ccagcgtttg cggcaacgtc aggccgagca cgtttttcac
    30781 atcacggttc agtaaccccg cgcgatccag ttcgccgaga ataccgataa caccaccagc
    30841 acggtgaaca tcttccatat ggtatttctg ggtgctcggc gcaactttac acagctgtgg
    30901 aaccttgcgg gaaagcttat cgatatcact catggtgaag tcgatttccg cttcctgcgc
    30961 cgccgccagc agatgaagta cggtgttggt agagccgccc atcgcgatat ccagcgtcat
    31021 ggcgttttca aacgccgcct tactggcgat attacgcggc agtgcactat cgtcgttttg
    31081 ctcgtaataa cgtttggtca attcaacaat gcgtttacca gcattgagga acagctgctt
    31141 acggtccgcg tgggttgcca gcaacgagcc gttgcccggc tgcgacaggc ccagtgcttc
    31201 ggtcaggcag ttcattgagt tagcagtaaa catcccggag caggaaccgc aggttgggca
    31261 ggcggatcgt tcaacctgat cgctctggga gtcagaaact ttcgggtctg caccctggat
    31321 catcgcatca accagatcga gcttaatgat ctgatcggac agtttggttt tcccggcttc
    31381 catcgggccg ccggaaacaa agatcaccgg aatattcagg cgcagggaag ccatcagcat
    31441 ccccggggtg attttgtcgc agttagagat gcagaccatg gcgtcggcgc agtgagcgtt
    31501 gaccatatac tcaacggaat cagcgatcaa ttcgcgagat ggcagtgaat aaagcatccc
    31561 cccgtggccc atggcaatcc catcatccac cgcaatggtg ttgaactctt tggcaacgcc
    31621 gccagccgct tcaatttgtt cggcgaccag tttaccgaga tcgcgcagat ggacgtgacc
    31681 cggtacaaat tgggtgaacg agttcacaac cgcgataatc ggcttaccga aatcggcgtc
    31741 ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc atattacgac catgagtggt
    31801 ggtggcggaa cggtacttag gcatacttta tttactccca gtgtctgtct cgtaaatggg
    31861 acggtgcgtg ccgtcccatt ttttgtattt attgattaac ttgatctaac cagccccatt
    31921 tatcttcggt ttcgccagtg aagaggccga agaaggcttg ctgaatgcgt ttggtaaccg
    31981 ggccgcaacg gccttcacca acctgaatac cgtctacact gcgcactggc gtgatttctg
    32041 ccgcagtacc ggacataaac acttcatccg ccaggtacag tgattcgcgc gacagcacct
    32101 gctcacgcac ttcaattccc agctctttcg ccagtttgat gatggcgtca cgggtaatac
    32161 ccggcagcgc ggatgaggtg aacggtgggg tgaacagcac accgtctttc acttcaaaca
    32221 ggttttcgcc tgcgccttca gagatgtaac cgttcacatc cagcgcgata ccttcctgat
    32281 aaccgtggcg gcgtgcttca ctacccacca gcagggaaga gaggtagtta ccaccggctt
    32341 ttgccgcggt tgggatggtg tttggtgctg cgcggttcca ggaggaaacc atcgcatcga
    32401 tcccctgctc cagcgcttct gcgcccagat acgcccccca cgggaaagcg gcgataatca
    32461 cgtcggttga gtatcccgct ggcgggttta cgcccatgcc aacatcacca acgaagatca
    32521 gcggacggat ataggcgctg gtgagattgt ttttgcggat cacgtcacga caagcttcca
    32581 tcagctcatc aatgctctgc gaaaccggga agcgatagat tttggcggag tcatgcagac
    32641 gctgcatatg ctcacgatgg cggaatacaa ccggtccttt gtgcgagtcg tagcaacgga
    32701 tgccttcaaa aaccgaggtg ccatagtgca gcgcgtgcga catcacatgc accttcgcgt
    32761 cttcccagcg aaccatctcc ccattgaacc aaatgtaatc agctttcttc gtggtcattt
    32821 ttatattcct tttgcgctca ggcgcggatt tgttgtgatg tggttgtgct ctggcagatg
    32881 gcaacgtgtg cgacgtccac cagtttattt aactgactaa acagtaagtc gaccgaccgt
    32941 gggctggcaa cggtcaattc gatatttata ttttgtgcat cgctggcggc ggccatattc
    33001 attgaacaga cgtggaaacc acgatgacgc accacgcgta aaacacgttc taaggtttcc
    33061 ggattgaagc gagccgatac attgacctga tgttgcatca tgataatttc tccaacattt
    33121 ctgaattact ggcaccaggc ggcaccagcg gccagacgtt ctcaagttcg tcgattgaga
    33181 catgaagcag gtatggccca tcactgttca gcatggtgtc gagtgccgct tcaacctggt
    33241 ctttatgggt gatgtgttgg ccagggatgc cgaaggcgct ggctaacatg aggaaatcgg
    33301 ggttatcggt aagggtggtt tcgctgtatc gttcctggaa aaacagttgc tgccattgtc
    33361 gaaccatccc taaccgttgg ttatcgagta agacgatttt caacggtaac tgcttgcgtt
    33421 ttacggtgcc cagctcttgc acattcatca tgaaagagcc gtcaccggag atacagacga
    33481 cagtatcgtt cggtcgcgcg acttgtgcgc caaccgccgc cggtaaaccg aaacccatgg
    33541 tgcctaagcc gctggaggtg atgaagtttt ccgggcgagt gtggacgatg tgctgggcag
    33601 cccacatctg gtgctgcccc acatctgtgg tcacgatgca atccgcagat ttacgatccg
    33661 acagttgttt taacaacaac ggcgcgtaga tagcgtcacc aggatggtcg taacgccagg
    33721 catgttcatc acgcagttgc gcgcagtgtt gctgccagtc atcgatattt aacggctgct
    33781 gtaatgctgg taacagagca tttaaatcac cctgtaatgc cacatgtgcc tgacgcagct
    33841 tgttcatttc tgccggatcg atatccatat ggataacgct ggcgtgtggt gcgaaggtgt
    33901 tcagtttgcc ggtcacccgg tcatcaaaac gtgcgcccac ggcgatcagt agatcgcact
    33961 cctgcactgc gaagttcgcc gctttggtgc cgtgcatccc cagcatgccc agatagtacg
    34021 gataatctgc ttctactgcg cccagccctt tcaacgtaca ggtggcaggc atttttgtgg
    34081 cagcgagaaa ttcacgcaaa gccgaaactg cctgcgccat gcccacgcca ccaccaacgt
    34141 acagcatcgg tttttgcgct tttgccagca tctgacgcgc ttgctcgact tcggcatgtg
    34201 ggaaagtcac ttcgttttca acagtggtga accacggttc cagatcgccg ctggctaact
    34261 ggatatcttt tggaatatcg accaaaaccg gaccaggacg accagagctg gcgacgtcaa
    34321 acgcttcggc catgatacgc ggtaactctt ccagtgattg caccaggaaa ctgtgcttgg
    34381 tgcaggctaa cgacaacccc agaatatcca cctcctgaaa tgcgtcagtg ccgataaacg
    34441 gtgcggatac ctgaccggtg atagcaacaa cggggatgga atctaacagc gcgtccgcaa
    34501 gcccggttat caggttggtt gcacctggac cagacgtggc gatgcaaacg cccgttttgc
    34561 cggtagcacg ggcataaccg atagccgcca ttgctgcacc ttgctcgtgt cggcacagca
    34621 agtgttccac gccaccgtca tacaatgcat cgtaaaccgg cataattgcg ccacccggat
    34681 aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg taccacccac tgtgcgccat
    34741 tcatagttag ttccccgtcc tgaatcttga gaaacagaat tttgtgctgt tattcattgt
    34801 ctgctcctca gttatgtttt taaggtcaaa aaaaaccccc ggacctttcg gtgcgggggt
    34861 cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc cccgcacggt
    34921 gggataataa tcaccaccac gctaatcacg accaggctaa tcactcgtag aagggctgtc
    34981 attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca tagattttgc
    35041 gaatagcaca agatattttt tggccgattt ttatgatgtc tttaataatt tcaagagaaa
    35101 ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaaatt caatttcagt atgaatgttg
    35161 ttcccttcct cgtagttcat cctttgcccg ctgcattttt gcgagcatca ttccaccgcc
    35221 agaacaactc aacttcactt gcacaaataa acaggaatca gccactaaaa ttcacgaatt
    35281 agcgggctac agcagcgata tcttatgcac ataatggtga ctcaaggagg gcttatgtca
    35341 ctgtcaattg ttcatacccg cgcagccctg ggagtaaatg cgcccccaat cactgttgag
    35401 gtacatatca gtaaaggtct accaggctta acgatggtgg gcttaccaga aacaacggta
    35461 aaagaagctc gcgatcgcgt gcgcagcgcc attatcaata gcggatatga atatccggcg
    35521 aaaaaaatca ccatcaacct tgctccagcc gatctgccaa aagaaggggg acgatatgat
    35581 ttgcctatcg ctattgcgtt actggcggcc tcagaacagc ttacagccaa taagttagat
    35641 gaatatgaat tagtcggaga actggcgctt acaggcgctc tgcgtggcgt tcccggcgca
    35701 atctccagtg caactgaagc cattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac
    35761 gaggatgaag tggggctaat taacggtgaa ggatgcctga tagccgatca tctgcaggct
    35821 gtctgtgcgt ttctggaggg taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta
    35881 tcccgggcgc tacaacatga tctcagtgat gttgtcggtc aggagcaagg aaagcgagga
    35941 ctggaaatta ccgccgctgg cgggcacaac cttttactga ttgggccgcc gggaacaggt
    36001 aaaacaatgc tcgccagccg tattaatggc ctgttgccag atttaagcaa tgaagaggca
    36061 ctggagagcg ctgcgatatt aagtctggta aatgctgaat cagtacaaaa acaatggcgg
    36121 cagcgcccat tccgctcacc tcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt
    36181 gcaattccag ggccaggtga aatttcgctg gcgcataacg gcgtgctttt tcttgatgag
    36241 ctacctgaat ttgaacggcg tacactggat gccttgcgag agccgattga atccgggcag
    36301 atccatcttt cacgcacacg agcaaaaata acctatccag cccgtttcca acttgttgcg
    36361 gcgatgaatc ccagccctac cggacattat cagggaaacc ataaccgctg cacaccagaa
    36421 cagacattac gttatctcaa ccggctctcg gggccctttc tcgaccgctt cgatctctca
    36481 ctagagatcc cattaccgcc ccccggcatt ttgagtaaaa cggtagtgca gggagaaaac
    36541 agcaccaccg ttaaacaacg tgtaatggcc gccagagagc gccaatttaa gcggcagaat
    36601 aaactgaacg cctggctgga taatccggaa atacgccaat tctgcaagct tgagagcgaa
    36661 gatgcgcagt ggctggaaga aacgctgatc catctggggt tatcgattcg tgcctggcag
    36721 cggttattga aagttgcacg aaccattgct gatattgatc agtctgacat tatcacacgt
    36781 cagcatttgc aggaggcagt tagctatcgt gcgattgacc gtttgctcat ccatctgcaa
    36841 aaactactga cataaaaaaa agggcatttc gcccttttta ttaatcgtca gaatcggtgt
    36901 agtcttcagc accttcaacc tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac
    36961 gcttgatacg cgtcatatac ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt
    37021 cgccacggca tactgctacg aagagtttct cttcctcggt aaccggctcg cgtttgccaa
    37081 gatccaactc attgaaggca taaccatgac gctcaagcag ttgtgcctct ttgatggtga
    37141 agtcaccatg acgagagaat ccacgtggat aatgtttatt gtcgaaatat cgattagtcg
    37201 tcgtaaagct ttccgccatc ctgcacgctc ctaattcttt gaccgagcta gttatggcgc
    37261 ggagtattag ttacgcttga cagagtgtaa aacaaaacat ttaaatcata acgacaaata
    37321 attttgcgga gagcactgtg gatacggaat tgttaaaaac tttcctggaa gttagccgaa
    37381 cgcgtcactt tggtcgagca gctgaatcgc tctatctgac ccaatcagcc gtgagctttc
    37441 gaatcagaca actggaaaat caactgggtg tgaacctttt cacccgccac agaaacaata
    37501 tccgtttaac cgctgctggt gaaaaactac tgccttatgc agaaacgctc atgagcacct
    37561 ggcaggccgc ccgtaaggag gtggcgcata cctcacgaca taacgagttt tctatcggtg
    37621 ccagcgcctc gttgtgggaa tgtatgctta atcagtggct gggacgcttg tatcaaaatc
    37681 aggatgtcca tacaggctta cagttcgaag cgcgaattgc ccaacggcag tctctggtaa
    37741 aacagctgca tgaacgccag cttgatcttc ttattactac tgaagcgccc aaaatggacg
    37801 aatttagcag tcagttgctc gggtatttca ctttagcgct ttataccagt gccccttcta
    37861 aactaaaggg agatcttaac tatctgcgac tggaatgggg gccagatttt caacagcatg
    37921 aggcaggttt gatcggtgct gacgaagttc ccattctgac aaccagttct gctgaactgg
    37981 cacagcaaca gattgcgacg cttaatggtt gcacctggct acccgtcagt tgggcgcgta
    38041 aaaagggcgg cctgcatacc gttgtcgata gcacaacact ttcacggccg ctttatgcca
    38101 tatggctgca aaatagcgat aaaaatgcgt tgattcgcga tctattgaaa attaacgtgc
    38161 tggatgaagt gtattaatct gaatggctgg caaggatgcc ggtagaagga tttacttcgg
    38221 agaggttatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggcagggg
    38281 cggagagact cgaactccca acacccggtt ttggagaccg gtgctctacc aattgaacta
    38341 cgcccctaat tagggtggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag
    38401 gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac caccggtttt
    38461 atgaccggct t
//
LOCUS       DAZKJR010000029        38081 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKJR010000029 DAZKJR010000000
VERSION     DAZKJR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.029"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(109..804)
                     /gene="aqpZ"
                     /locus_tag="ACHOWS_001551"
     CDS             complement(109..804)
                     /gene="aqpZ"
                     /locus_tag="ACHOWS_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFQ0047144.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAAAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1230..2888
                     /gene="ybjD"
                     /locus_tag="ACHOWS_001552"
     CDS             1230..2888
                     /gene="ybjD"
                     /locus_tag="ACHOWS_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFQ0047145.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2885..3841)
                     /gene="ybjX"
                     /locus_tag="ACHOWS_001553"
     CDS             complement(2885..3841)
                     /gene="ybjX"
                     /locus_tag="ACHOWS_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFQ0047146.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYKGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3992..5107
                     /gene="macA"
                     /locus_tag="ACHOWS_001554"
     CDS             3992..5107
                     /gene="macA"
                     /locus_tag="ACHOWS_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFQ0047147.1"
                     /translation="MKKRKTVKKRYVIALVIIIVGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5104..7050
                     /gene="macB"
                     /locus_tag="ACHOWS_001555"
     CDS             5104..7050
                     /gene="macB"
                     /locus_tag="ACHOWS_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFQ0047148.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7123..7347)
                     /gene="cspD"
                     /locus_tag="ACHOWS_001556"
     CDS             complement(7123..7347)
                     /gene="cspD"
                     /locus_tag="ACHOWS_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFQ0047149.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7497..7571
                     /gene="yljB"
                     /locus_tag="ACHOWS_001557"
     CDS             7497..7571
                     /gene="yljB"
                     /locus_tag="ACHOWS_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFQ0047150.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7670..7990
                     /gene="clpS"
                     /locus_tag="ACHOWS_001558"
     CDS             7670..7990
                     /gene="clpS"
                     /locus_tag="ACHOWS_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFQ0047151.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8021..10297
                     /gene="clpA"
                     /locus_tag="ACHOWS_001559"
     CDS             8021..10297
                     /gene="clpA"
                     /locus_tag="ACHOWS_001559"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFQ0047152.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10820..10907)
                     /locus_tag="ACHOWS_001560"
     tRNA            complement(10820..10907)
                     /locus_tag="ACHOWS_001560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10871..10873),aa:Ser,seq:gga)
     gene            complement(11161..11379)
                     /gene="infA"
                     /locus_tag="ACHOWS_001561"
     CDS             complement(11161..11379)
                     /gene="infA"
                     /locus_tag="ACHOWS_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0047153.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11664..12368)
                     /gene="aat"
                     /locus_tag="ACHOWS_001562"
     CDS             complement(11664..12368)
                     /gene="aat"
                     /locus_tag="ACHOWS_001562"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFQ0047154.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12410..14131)
                     /gene="cydC"
                     /locus_tag="ACHOWS_001563"
     CDS             complement(12410..14131)
                     /gene="cydC"
                     /locus_tag="ACHOWS_001563"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFQ0047155.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAALEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFNYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHNAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14132..15898)
                     /gene="cydD"
                     /locus_tag="ACHOWS_001564"
     CDS             complement(14132..15898)
                     /gene="cydD"
                     /locus_tag="ACHOWS_001564"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFQ0047156.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(16021..16986)
                     /gene="trxB"
                     /locus_tag="ACHOWS_001565"
     CDS             complement(16021..16986)
                     /gene="trxB"
                     /locus_tag="ACHOWS_001565"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0047157.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17530..18024
                     /gene="lrp"
                     /locus_tag="ACHOWS_001566"
     CDS             17530..18024
                     /gene="lrp"
                     /locus_tag="ACHOWS_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFQ0047158.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18159..22226
                     /gene="ftsK"
                     /locus_tag="ACHOWS_001567"
     CDS             18159..22226
                     /gene="ftsK"
                     /locus_tag="ACHOWS_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0047159.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAAEQSAQQPYYAPA
                     PEQTAAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQHPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAHHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22385..22996
                     /gene="lolA"
                     /locus_tag="ACHOWS_001568"
     CDS             22385..22996
                     /gene="lolA"
                     /locus_tag="ACHOWS_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFQ0047160.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSNFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            23007..24350
                     /gene="rarA"
                     /locus_tag="ACHOWS_001569"
                     /gene_synonym="mgsA"
     CDS             23007..24350
                     /gene="rarA"
                     /locus_tag="ACHOWS_001569"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFQ0047161.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24441..25733
                     /gene="serS"
                     /locus_tag="ACHOWS_001570"
     CDS             24441..25733
                     /gene="serS"
                     /locus_tag="ACHOWS_001570"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0047162.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25972..28416
                     /gene="dmsA"
                     /locus_tag="ACHOWS_001571"
     CDS             25972..28416
                     /gene="dmsA"
                     /locus_tag="ACHOWS_001571"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFQ0047163.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28427..29044
                     /gene="dmsB"
                     /locus_tag="ACHOWS_001572"
     CDS             28427..29044
                     /gene="dmsB"
                     /locus_tag="ACHOWS_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFQ0047164.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            29046..29909
                     /gene="dmsC"
                     /locus_tag="ACHOWS_001573"
     CDS             29046..29909
                     /gene="dmsC"
                     /locus_tag="ACHOWS_001573"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFQ0047165.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29945..30571)
                     /gene="ycaC"
                     /locus_tag="ACHOWS_001574"
     CDS             complement(29945..30571)
                     /gene="ycaC"
                     /locus_tag="ACHOWS_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFQ0047166.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30886..32034
                     /gene="ycaD"
                     /locus_tag="ACHOWS_001575"
     CDS             30886..32034
                     /gene="ycaD"
                     /locus_tag="ACHOWS_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0047167.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32244..33674
                     /gene="ycaM"
                     /locus_tag="ACHOWS_001576"
     CDS             32244..33674
                     /gene="ycaM"
                     /locus_tag="ACHOWS_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0047168.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33675..33785)
                     /locus_tag="ACHOWS_001577"
                     /pseudo
     CDS             complement(33675..33785)
                     /locus_tag="ACHOWS_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33884..34624)
                     /gene="pflA"
                     /locus_tag="ACHOWS_001578"
     CDS             complement(33884..34624)
                     /gene="pflA"
                     /locus_tag="ACHOWS_001578"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFQ0047169.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMREVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34816..37098)
                     /gene="pflB"
                     /locus_tag="ACHOWS_001579"
     CDS             complement(34816..37098)
                     /gene="pflB"
                     /locus_tag="ACHOWS_001579"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0047170.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37153..38010)
                     /gene="focA"
                     /locus_tag="ACHOWS_001580"
     CDS             complement(37153..38010)
                     /gene="focA"
                     /locus_tag="ACHOWS_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFQ0047171.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9390 a         9304 c        10144 g         9243 t
ORIGIN      
        1 ctgccccggc cttcggggaa cgcttcagcg attttgacgc caccaacacc cgtgcggttt
       61 gtaacgtcga agataaaaca accagggcag ataaaatgcc aatgctaatt aattacgctt
      121 ttccagcagg gtccggtaaa tcagaccacc gataataccg ccgacaattg gcaccaccca
      181 gaagaaccac agttgttcta acgcccagcc gccctggaag atagcagccg cggtgctgcg
      241 cgccgggtta acagaagtgt tagtcaccgg aatactgatt aagtgaatca gggttaaggc
      301 cagaccaata gcgatcggcg caaaaccagc aggagcgaat ttgtcggttg cgccgtggat
      361 caccagcagg aaacctgcac tcaataccag ttcaactacc agcgcggaaa gcatggaata
      421 accgcctggc gaatgctcgc cataaccgtt agaagcaaaa ccgctggctg ccgcgtcaaa
      481 acctgtttta ccactggcaa ttaaatacag cagcgccgcc gcaacaatac cgccgacaac
      541 ctgggcaatt acgtagccaa cgacttcttt tgccgggaaa cgtccgccag cccataaacc
      601 aatagtgacc gccgggttaa aatgaccacc agaaatatga ccaacagcaa aggccatcgt
      661 cagaacggtc agaccgaatg ccagcgccac gccggcaaaa ccaatgccta attccgggaa
      721 gcctgcggcc agtacggcac taccacagcc accaaaaaca agccagaaag taccaaaaca
      781 ttcagctgct aattttctga acatatccac ctcaattaaa aattgaccct gtgaaaaata
      841 tggtcgtttt atagggtcgt cgtaaaaaag tgacgacgga aataatgcgc ggctatttta
      901 aaaacgaagg cgagtcattc accagataaa taaatccagt aaatttgatt taaggcaaca
      961 gcgggttgcc ccatatagtc atttgtctga ttgacagtgt agtgcaagcg aaagatttaa
     1021 tcctttaggc gtaataaaaa ataatttatc atgctaatta tttgattttg ttgtttttgc
     1081 agacttatca gcaagaggga gtataacgcg attattcgct catttttcag acatttgcca
     1141 tgcttaaatg tgatgtcatc acgtattagc atggcctttc ccgttatact gccagcgtaa
     1201 aggataagtc acatatttct ggaggggata tgattcttga gcgcgttgaa attgtgggtt
     1261 ttcgcggtat caaccgtttg tcgttgatgc tggaacaaaa caacgtcctg attggggaga
     1321 acgcgtgggg taaatccagc ttgctggacg ccttaaccct gctgctatcg ccagaatcag
     1381 atctctacca ttttgagcgc gacgatttct ggttcccgcc gggagatatc aacgggcgag
     1441 aacatcatct gcatattatt ttgaccttcc gcgaatcgct gccaggccga catcgggttc
     1501 gccgctatcg gccgctggaa gcgtgctgga cgccatgcac cgatggttat caccgtattt
     1561 tttatcgtct ggaaggggag agtgcggaag acggcagcgt gatgacactg cgcagttttc
     1621 tcgataaaga cggacatccg attgatgttg aggatattaa cgatcaggct cgtcatctgg
     1681 tgcgtttaat gccggtgctg cgcttgcgtg atgcccgttt tatgcgccgt attcgtaacg
     1741 gcacggtgcc aaatgtcccc aatgtggaag tcaccgcgcg ccagctcgat ttcctcgccc
     1801 gtgaattatc ctcacatccg caaaatctct ctgatgggca gattcgtcag ggactttccg
     1861 caatggtgca acttcttgag cattattttt ctgagcaggg agctggacag gcgcgatatc
     1921 gtttaatgcg gcggcgagcc agcaatgagc aacgaagctg gcgctatctg gatatcatca
     1981 accggatgat tgaccgacct ggagggcgct cgtatcgggt tattttgctc ggcctgtttg
     2041 ctactttatt gcaggcaaaa ggcacattgc gactggataa agacgcccgt ccattgttgc
     2101 tgatcgaaga tccagaaacc cgtttacacc ccattatgct ttcagttgcc tggcatctgt
     2161 tgaatcttct gcccttgcag cgcattgcca ccaccaactc gggtgagtta ctttcgttaa
     2221 cgccggtaga gcatgtttgc cgactggtac gtgagtcctc gcgcgttgcc gcctggcgtc
     2281 tggggccgag tggcttgagt accgaagata gccgacgcat atcctttcac attcgtttta
     2341 accgtccgtc atcgctgttt gctcgctgct ggctgctggt ggaaggggaa acggaaacct
     2401 gggttatcaa tgaactggcg cgtcagtgcg gacatcattt tgatgccgaa ggaatcaagg
     2461 tcattgagtt tgcccagtcc gggctaaagc cactggttaa atttgcccgc cgaatgggga
     2521 ttgaatggca tgtactggtc gatggcgatg aagcagggaa gaaatatgcc gctacggtac
     2581 gcagcctgtt gaataacgat cgggaagccg aacgagaaca tttaacggcg ttaccggcgc
     2641 tggatatgga acattttatg tatcgccagg gattttccga tgtgttccac cgcgtggcgc
     2701 aaatcccgga aaatgtaccg atgaatctgc gcaaaattat ctcgaaagcg atccatcgct
     2761 cttccaaacc cgatcttgcc attgaagtgg caatggaggc cggacgtcgt ggtgtggact
     2821 ccgtaccgac gctgctgaaa aaaatgttct cacgcgtgct gtggctggcg cgcggtcgcg
     2881 cggattaacc gcgaaacatc gtggccattt gtggctgaat agcgtcgagc atctcatagc
     2941 gccgacggta ttcagcccgt tttttactgg cgatttcggc aatctctttt cgtgctatct
     3001 gtgccggaag gcggtaatgg cgttcagcat cacatacgcc gccaaccgac tcccagaacg
     3061 cgttgtaatc ggcatggatc ttgccttctt tatcacgata acgcaggctg cggtaaatat
     3121 gcgtttcatt gctgacggca ataatctgct ctacctgcaa acgttgggca aacagacagg
     3181 ccgcttccat cacaaggcgt ttgggaaata gcccgtggca ggctttcgtc gcattctgga
     3241 tttcctgatg tggaatttcc cattttgcgc cttgcagtcc gccaataaac attgttcttt
     3301 tccccttata ttcacacagg gtaaacgtga tctctgccag aggaatacct tcgctgttgc
     3361 ggaacaggat tgtgctgtca ccttctttat ccattgagat catcatggtc agctcaagcg
     3421 tgaactgctc gccgtttttg ccttccagtt tagccagttg cagcccgggg gtattcaaat
     3481 ataagctgaa ttcttccgcc gacatacacc cgcggagtaa cgcataatgg taacgtaacg
     3541 cctccagcaa ttgcttacgg ctaagattcg ccgcaaggta agggcgatgc agacgcacag
     3601 gcagtcgcgg ctggcgcgtt aacaacacat tgagattggg ccagtgggaa agttcgttca
     3661 tccactcaac gcttaaacgc ggcataatca acgagcgcag caaaaatttc tggcgaaaac
     3721 tacggcgatg ccagaattta cccggccgac actgtccacg tgccagacta agaaaaagtg
     3781 acaggctgct gagagattca gatggcgtaa aggtccgttc agttagctgc gacatattca
     3841 tgaaatcaat ggttatacat gacgtcgatt tcaccattgc gtatcttaac caaacatcaa
     3901 tagtgtgatt actaatgtaa attttagggt tttgttgata tttcgttgaa gttaatgacc
     3961 cggattggca tatggagtat tcagaatatt tatgaaaaag cggaaaaccg tgaagaagcg
     4021 ttacgttatt gcgctggtga taatcatcgt cgggctgatt acgttatgga gaattcttaa
     4081 cgcacccgtg ccgacttatc agacgctgat tgtgcgcccc ggtgatttac agcaaagcgt
     4141 gctggcgacc ggaaagctgg acgcgctgcg taaggttgat gtgggcgcgc aggtcagcgg
     4201 tcagttgaaa acgctgtcgg tggcgattgg cgataaagta aaaaaagacc agcttttagg
     4261 ggttattgat cctgaacagg ctgaaaacca gattaaggag gtggaagcaa cgctgatgga
     4321 gctacgcgcg cagcggcagc aggcggaagc ggagctgaaa ctggcgcggg tgacgtattc
     4381 ccgtcagcaa cgtcttgcac aaacgcaagc ggtttcacag caggatctcg ataacgccgc
     4441 gacggagatg gctgtgaaac aggcgcaaat tggcaccatt gacgcgcaaa tcaagcgcaa
     4501 tcaggcctct ctcgatacgg ctaaaaccaa tctcgattac acccgcatcg ttgccccgat
     4561 ggccggggaa gtcacgcaaa tcaccactct gcaaggccag acggtgattg ccgcacaaca
     4621 agcaccgaac attctgacgc tggcagatat gagcaccatg ctggtaaaag cgcaggtttc
     4681 tgaagcggat gtaatccacc tgaagccggg gcaaaaagcc tggtttacgg tgcttggcga
     4741 tccactgacg cgctacgagg ggcaaatcaa ggatgtgcta ccgacgccgg aaaagattaa
     4801 cgacgctatt ttctattacg cccgttttga agtacccaac cccaatggtt tactgcggct
     4861 ggatatgacc gcgcaagtgc atattcagct caccgatgtg aaaaatgtgc tgacgatccc
     4921 tctgtcggcg ttaggcgatc cggttggcga taatcgttat aaagtcaaat tgttgcgtaa
     4981 tggtgaaaca cgcgagcgtg aagtgacgat tggcgcacgt aacgataccg atgttgagat
     5041 tgtcaaaggg ctggaagcgg gcgatgaagt ggtgattggt gaggccaaac caggagctgc
     5101 gcaatgacac ccttgctcga attaaaggat attcgtcgca gctatcctgc cggtgatgag
     5161 caggttgagg tgctgaaggg catcagcctc gatatttatg ctggtgagat ggtggcgatt
     5221 gtcggcgctt cgggttccgg taaatcgacc ctgatgaata ttctcggctg cctggataag
     5281 gccaccagcg gcacctatcg cgtagccggt caggatgttg ccacgctgga cgccgatgcg
     5341 ctggcgcaac tacgccgcga gcatttcggc tttattttcc agcgttacca tttgctttcg
     5401 catttaaccg ccgagcagaa cgttgaagta cccgccgtct atgccggtct tgagcggaaa
     5461 cagcggctgc ttcgcgccca ggagttgctg caacgactgg ggctggaaga ccgtacagag
     5521 tattacccgg cacagctttc gggtggtcag caacagcgcg tcagcatagc gcgggcattg
     5581 atgaacggcg gtcaggtaat tcttgccgat gaaccgaccg gcgcactgga cagccattct
     5641 ggcgaagagg tgatggcgat cctgcatcag ctgcgcgatc gcgggcatac ggtgattatc
     5701 gttacccacg atccgcaggt cgctgctcag gccgagcggg tgatcgaaat tcgcgacggt
     5761 gaaattgtgc gcaatcctcc cgccattgaa aaagtgaatg ttgctggcgg gacggaacct
     5821 gttgtcaaca cggcgtctgg ctggcggcag tttgtcagcg gttttaacga ggcgctgacg
     5881 atggcatggc gggcgctggc agcgaataaa atgcgtactt tactgaccat gctggggatt
     5941 attatcggta ttgcgtcggt ggtttccatt gtcgtggtgg gtgacgccgc caaacaaatg
     6001 gtgctggcgg atattcgttc tattggtacg aatactattg atgtctatcc cgggaaagat
     6061 tttggcgatg acgatccgca atatcagcag gcgctgaagt acgacgactt aatcgccatc
     6121 caaaaacaac cgtgggtcgc ctcagccaca cctgccgtct cgcaaaacct gcgcctgcgt
     6181 tataacaatg ttgatgtggc tgccagtgcc aatggcgtga gcggcgatta ttttaatgtc
     6241 tatggcatga ccttcagtga agggaacacc tttaatcagg agcagctgaa cggtcgtgcg
     6301 caggtggtgg tgctcgacag taatactcgc cgccagcttt tcccccataa agcagatgtg
     6361 gttggcgagg tgattctggt cggcaatatg cccgccagag tcattggtgt ggcggaagaa
     6421 aaacagtcga tgtttggtag cagtaaagtg ctgcgtgtct ggctacctta cagcacgatg
     6481 tccgggcgag ttatgggcca gtcgtggctg aactccatta ctgtcagggt gaaagaaggc
     6541 tttgacagcg ctgaggcgga acagcaactc acgcgtttac tttcactgcg ccacggtaag
     6601 aaggatttct ttacctggaa catggacggc gtcttgaaaa ccgttgaaaa gaccacacgt
     6661 actttacaac tgtttctgac gctggtggcg gtgatttcgc tggtggtggg cggtattggt
     6721 gtaatgaata ttatgctggt gtcagtgacc gagcggacgc gggaaattgg cattcgcatg
     6781 gctgtaggtg cgcgagcaag cgatgttttg caacagttcc tgattgaagc cgtactggtt
     6841 tgcctggtcg gtggcgcgtt gggaataaca ctgtcactgt taattgcttt caccttacag
     6901 cttttcttac ccggctggga gattggtttt tcaccgttgg cgctgctgct ggcgtttctc
     6961 tgctcgacgg tcaccgggat tttatttggc tggttacccg cacgaaatgc ggcacgactg
     7021 gatccagtag atgctctggc acgagagtaa tttttgagat aaaaatgcca gccgatcggg
     7081 ctggcatttt gcctttagga tgtacacaat gagacagaag agctatgcga ctgccgcttc
     7141 tacttcgacg ggcacaataa cactggcgtg attgcctttt ggcccctggt ggacatcaaa
     7201 ctgaacggat tgtccagctt ttagcgttct gtaaccatcc atctgaatgg tggaataatg
     7261 agcgaaaata tcttcgccgc cgccttcagg gcagatgaaa ccaaaccctt tggcattgtt
     7321 gaaccactta acagtaccct tttccatgct tcgacatcct tcgcaaatct tatacaagta
     7381 agatggaata aaccggggtc agagaggggg ctgttcaaaa cctcgccaac tctagaaata
     7441 caatttagag aattagggcg agccgtcaag catttgacag gggccagggg gcaggtatga
     7501 atcaaaaatt tgaagcagtt aacgctattg acaggaatgt gacagatgtc gctgatgcca
     7561 acgatagatg atagttatct atcatgtgga gtagattggt caggcaaata agctcttgtc
     7621 agcggcaggg cgttctgccg ataaccgtaa ccgaagatga taactgacaa tgggtaaaac
     7681 gaacgactgg ctggactttg atcaactggc ggaagaaaaa gttcgcgacg cgctaaaacc
     7741 gccatctatg tataaagtga tattagtcaa tgatgattat actccgatgg agtttgttat
     7801 tgacgtgtta caaaaattct tttcttatga tgtagaacgt gcaacgcaat tgatgctcgc
     7861 tgttcactac caggggaagg ccatttgtgg agtctttacc gctgaggttg cagaaaccaa
     7921 agtggcgatg gtgaacaagt acgcgaggga gaatgagcat ccattgctgt gtacgctaga
     7981 aaaagcctga atgcaggcat aaaaattggg ggaggtgcct atgctcaatc aagaactgga
     8041 actcagttta aatatggctt tcgccagagc gcgcgagcac cgtcatgagt ttatgaccgt
     8101 cgagcacttg ttactggcgc tgctcagtaa cccatctgcc cgggaggcgc tggaagcgtg
     8161 ctctgtggat ttggttgcgc tccgtcagga actggaagcc tttattgaac aaaccacacc
     8221 cgttctgcct gccagtgaag aggagcgcga cacacagccg acgctgagtt ttcagcgtgt
     8281 actgcaacgt gcggtcttcc atgtccagtc ctccggtcgc aatgaggtta ccggtgcaaa
     8341 cgttctggtc gctatcttta gcgaacagga gtcgcaggcg gcatatctgt tgcgcaaaca
     8401 tgaagtcagc cgtctcgatg tggtgaactt tatctctcat ggcacgcgta aagacgagcc
     8461 gacacagtct tctgatcctg gcagccagcc aaacagcgaa gaacaagctg gtggggagga
     8521 acgtatggag aatttcacga cgaacctgaa tcagcttgcg cgagtgggcg gaatcgaccc
     8581 actgattggt cgtgagaagg agctggagcg tgctattcag gttctctgcc gtcgccgtaa
     8641 aaacaacccg ctgctggtgg gggaatctgg tgtcggtaaa accgcgattg cggaggggct
     8701 tgcatggcga attgttcagg gcgatgtgcc ggaagtgatg gctgactgta cgatttactc
     8761 tctcgatatc ggttctctgt tagcgggcac aaaatatcgc ggcgactttg aaaaacgttt
     8821 taaagcgttg ctcaagcagc tggagcaaga cactaacagc atcctgttta ttgatgagat
     8881 ccacaccatt atcggtgcgg gtgcagcgtc tggtggccag gtcgatgcag ctaacctgat
     8941 caaaccgttg ctctccagcg gtaaaattcg cgtaattggt tcgacaacct atcaggagtt
     9001 cagcaacatt ttcgagaaag accgtgctct ggcgcgtcgc ttccagaaaa ttgatattac
     9061 tgaaccgtca atcgaagaaa ctgttcaaat catcaatggc ctgaaaccga agtatgaagc
     9121 gcaccacgac gtgcgttata ccgcaaaagc ggtgcgtgca gcggtagagc tggcggtgaa
     9181 atacattaac gatcgtcatc tgccggataa agccattgac gttatcgacg aagcgggcgc
     9241 tcgcgcacgc ctgatgccgg taagcaaacg caagaaaacc gttaatgtgg cggatattga
     9301 gtccgtggtg gcccgtattg cgcgcattcc agagaagagt gtttcgcaga gtgaccgcga
     9361 taccctgaaa aacctcggcg atcgcctgaa aatgctggtc ttcggtcagg ataaagccat
     9421 tgaggcgctg actgaagcca ttaagatggc gcgtgcaggt ttaggtcacg aacataaacc
     9481 ggtcggttcg ttcctgtttg ccggtcctac cggggtcggg aaaacagagg tgacggtaca
     9541 gctttcgaaa gcgttgggca ttgagcttct gcgctttgat atgtccgagt atatggaacg
     9601 ccataccgtc agccgtctga ttggtgcgcc tccgggatac gttggttttg atcagggcgg
     9661 tttgctgact gatgcggtca tcaagcatcc acatgcggtg ctgctgctgg acgaaatcga
     9721 gaaagcgcac ccggacgtgt tcaatattct gttgcaggtg atggacaacg gtacgctgac
     9781 cgataacaac ggacgcaaag cggacttccg taacgtggta ttggtgatga ccaccaacgc
     9841 tggggtacga gaaactgagc gtaaatcgat tggtcttatc caccaggaca acagtaccga
     9901 tgcgatggaa gagatcaaga agatctttac gccggagttt cgtaaccgtc tcgacaacat
     9961 tatctggttc gatcatctct ccaccgacgt gatccatcag gtagtggata aattcatcgt
    10021 cgagttgcag gttcagctgg atcagaaagg tgtttctctg gaagtgagcc aggaagcgcg
    10081 taactggctg gccgagaaag gttacgaccg ggcaatgggc gcacgtccga tggcgcgtgt
    10141 catccaggac aacctgaaaa aaccgctcgc caacgaactg ttgtttggtt cgctggtgga
    10201 cggcggtcag gtgacggttg cgctggataa agagaaaaat gagctgactt atggattcca
    10261 gagtgcacaa aagcacaagg cggaagcagc gcattaatct aattgttagg taggttggtg
    10321 aagtccgtaa tctcgaaaga ggttacggac tttttgttta tggggtggag gaggttctga
    10381 cccttttttt gatgatgatg gtaatttgtt gataattagt gctgcgggaa ggtaaggata
    10441 aaaaagggtg gtagcaggaa aatgagatgg ttttgcttta ttaacaacgg gctagacgtg
    10501 tagtatttga gttcactgcc gtacaggcag cttagaaagc caaccgaaag aaaaactcga
    10561 tttttatacc gttcactgcc gtacagacag ataaaatgcg aaaaaaaaaa cgtactttcg
    10621 tacgagctct tctttgaata tggcggtgag ggggggattg actcgcttcg ctcgccctgc
    10681 gggcagccca ctcactgtgt tcgtggtctg tccaactggc tgcgccagtt gtcgaacccc
    10741 ggtcggggct tctcatcccc cctggagtgc aatatgcgaa aaaaaagctc gcactttcgt
    10801 acgagctctt ctttgaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
    10861 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
    10921 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
    10981 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
    11041 catcgagaca agttatggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    11101 aggccggcta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    11161 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    11221 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    11281 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    11341 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    11401 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    11461 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    11521 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    11581 tccttatcgg cagcggggta atgagcgtaa ccaactctgc gaccgcaatt ataacactct
    11641 ggggagaaat gtgccgaaaa cattcattct tgtggtgaaa acaagcaccg tggtacccag
    11701 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattgaggta atcccggcgg
    11761 ggaatttcgc aggcaccaag cgacgctgtg tgatcgttaa ggacctggca gtcgataagt
    11821 ttaccgccat gaccaataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    11881 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    11941 ccgccgacaa gctcatcttc acgccagact tcaatagagt gggcatgacc gagttcgtga
    12001 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    12061 gcacagcctt caatgacctg accaaaagcg taattcatcg tgactcgata gggcgagcgt
    12121 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcgg
    12181 ggatcgggcg accaccagag gatggggtca cctggagaaa accacggaaa aataccacgc
    12241 tggtaagcca ttaacaggcg cgcagggcta agatcgcctc ccagtgccag caggccgtta
    12301 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    12361 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    12421 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    12481 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    12541 gggtgaccat taacaccgtt ttctcatgca tcatttctgc aagcaattca aggatctgac
    12601 tttcggttgt ggcatctaag ccttctgtag gttcatccag caataccagc ggcgcattat
    12661 gtaacagcgc acgggcgata gccagacggc gtagttcacc accggagagc tggcgtccgc
    12721 cttcacccaa ccaactgttg agacctgcat cctcgagcag cttctccaga ccaacgcgac
    12781 gtaagatctc cgccagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    12841 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    12901 tcgcttcatt caggctggct atggggctac cgttaagcaa aatctcgccc tgttgcgggt
    12961 cccatgcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    13021 tcgctatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    13081 gctccggata attgaattga acatcccgta acgtcagcga aacgcgatcg gcaacacgag
    13141 tttgggtatc agggaaggtg acttccggtt tttgatccgt taagtcagtg atccgtacgg
    13201 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    13261 gcgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    13321 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    13381 ttatcgcttg cgacaatgcg gtgagttcag attgacgacg ttgcgcttcc agccactgaa
    13441 tttctgtatt ctctaattgc gtgcgataac gatcgctggc accaaagatg gtcagctcag
    13501 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg cccgcgaaga tgagtcagat
    13561 tttgcccggt gctttttccc gcacgataaa acagtggtgg cataaggaaa agcgtcagta
    13621 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgattgtca
    13681 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    13741 cgagcgtatc aacatccgcc accacgcggt tgagcaattc gccctgacga tagcgcgcca
    13801 gtccggcagg ggataggggc agcaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    13861 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata gcgcccggca gtacgggtga
    13921 ttgctgcgcc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa
    13981 cccccgctac cgctgaggcg gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    14041 cgagcagcgt gacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    14101 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagca
    14161 atgtggcgaa aggaccacca gccacgctta attccgcgta acgtccttgc tcaataatct
    14221 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    14281 ccattaacgt tgtctggcgc agagaggcgg cattaagcgc ctccattacg cgctgttcac
    14341 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    14401 atgctcgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    14461 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    14521 ccagcgctgt ttgtaactcg tgttcgctgg catcaggtcg tgccagtagt acgttatccc
    14581 gtagtgttgc tgccggtaat tgtgggtttt gcccaaccca ggagagatgc ttacgccatg
    14641 attccggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    14701 gaaaaccaga aagcgcgttc agcagtgagc ttttacctga accgctgcga ccaaccaaca
    14761 ctgcacgttg gcctgctggc aaagtaaagt tcagcggtcc agccagcgtt ttaccttctg
    14821 gtgatgtaat aaacagatcc tcggcttcaa tggtcaccgg atcggttgat gctaattccg
    14881 cctcaccacg ttgcggatgg gctagcgggg tttccataaa cgttttcaga ctgtcagctg
    14941 cgccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    15001 actccggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcatagt
    15061 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    15121 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa cgccagccgt agcacttcca
    15181 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    15241 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    15301 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    15361 taaacaacgg aattaacggt gcagtgccca gcagaataag cgccgcagcc cagttagacg
    15421 ggaagatcgc caccacaatc agcaaaggca cggacactgc cagcgccatt tgcggcaggt
    15481 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    15541 ccgccggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    15601 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    15661 ccacccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    15721 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    15781 caatgatcaa tatgccgctc acaaagccca gcagacgaga aatattcagc caacgttggg
    15841 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgggat ttattcattg
    15901 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac gaggaaaaaa
    15961 taaaggcgac ccatagtcgc atggtgtcgc cttctttact tttgttactg atttgtaaaa
    16021 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    16081 tgtaccagca gatgtaatag cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    16141 gacgccaggg atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    16201 gccgttttcc agttccagct gcccttcgaa aatagccgta ttcgggctgt gaccgatagc
    16261 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    16321 acgaacgcca gtgacgccca tctgatcgcc ggtcacctct tccagcgtac ggttggtgtg
    16381 tagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    16441 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    16501 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    16561 gaaaccgtcg caggtagcac aagcagaaac cccacggcct ttaaaggctt cttcagatgg
    16621 caggcctaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    16681 gccgctatcg ccagtcagac ggaacggacg attctgcaaa tcaactttgt tgatgtgatc
    16741 aaagatgatt tcagtttcaa atttggtggc atgttcgtgc atgcgctcca ttaataacgg
    16801 accggtcaga tcgtttggat cgccaggcca gttttccact tccgtggtgg tggtcagttg
    16861 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    16921 agcagcggta tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    16981 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    17041 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    17101 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    17161 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    17221 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaacagta
    17281 ttcttatatg cgcataacca tgcatgtaaa taccatgttt accgtgctag tgaaatctac
    17341 gtatggcgtg gacagacgcc attcgtgatg tcgatagctg ccacaaggca acggtcttct
    17401 caccgtagac ccaggcattg cgcgccgtga atcttcatga tttcggtcta tcgtgacggg
    17461 tagcgactct gaacagtgat gtttcagggt cagacaggag tagggaagga atacagagag
    17521 acaataataa tggtagatag caagaagcgc cctggcaaag atctcgaccg tatcgatcgt
    17581 aacattctta atgagttgca aaaggatggg cgtatttcta acgtcgagct ttctaaacgt
    17641 gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt
    17701 attcagggct atacggcgct gctgaacccc cattatctgg atgcatcact tctggtattc
    17761 gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa taccgctgta
    17821 caaaaacttg aagaaattca ggagtgtcat ttagtatccg gtgatttcga ctacctgttg
    17881 aaaacacgcg tgccggatat gtcagcttac cgtaagttac tgggggaaac cctgctgcgt
    17941 ctgcctggcg tcaatgacac acggacatac gttgttatgg aagaagtcaa gcagagtaat
    18001 cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta ttttgattac
    18061 actcctgtta atccatacag caacagtact ggggtaacct ggtactgttg tccgttttag
    18121 catcgggcag gaaaagcctg taacctggag agcctttctt gagccaggaa tacactgaag
    18181 acaaagaagt cacattgaca aagttaagta gcggccgccg ccttctggag gcgttgctga
    18241 tccttattgt cctgtttgcc gtctggttga tggctgcctt actaagcttt aacccttcgg
    18301 accccagctg gtcgcaaacg gcctggcatg aacctatcca taatttaggt gggatgcccg
    18361 gtgcgtggct ggcagatacg ctgttcttta tttttggcgt gatggcttac accattcccg
    18421 tcattattgt cggcggttgt tggtttgcct ggcgtcatca gtccagcgac gaatacattg
    18481 attattttgc cgtttcgcta cgcatcattg gcgttttggc gctcatcctt acctcctgtg
    18541 gtctggcggc aatcaacgct gacgatatct ggtattttgc ctccggtggc gtcattggca
    18601 gcttactaag cactacgcta caaccactgc tacacagtag cgggggaact attgcgctgc
    18661 tctgcgtttg ggctgcgggt ctgacgctgt tcaccggttg gtcatgggtg accattgctg
    18721 aaaaactcgg cggctggatt ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg
    18781 atgatacctg ggtcgatgaa gatgagtatg aagacgacga agaatatgaa gatgaaaatc
    18841 acggtaaaca gcatgaatca cgccgtgccc gtattcttcg cggcgcgtta gcgcgtcgta
    18901 aacggttggc ggaaaaattc attaatccga tggggcggca aacagatgct gcgttgttct
    18961 ccggtaagcg gatggatgat gaagaagaga ttacctacac tgcacgcggt gtggctgccg
    19021 accctgacga cgtcctcttt tcgggcaatc gtgcaacgca gccagaatat gacgaatacg
    19081 atccattatt aaacggtgcg ccaattaccg aacctgttgc tgtagcagct gctgctacca
    19141 cggcgacaca aagctgggct gcgccggttg aacctgtgac tcagacgccg cctgttgcct
    19201 ctgttgatgt tccaccttcg caacctacag tagcctggca gcctgtaccg ggtccacaaa
    19261 cgggagagcc ggtgattgct ccggcaccgg aaggttaccc acagcagcca caatatgcgc
    19321 agcctgcagt gcaatataat gagccgctgc aacaacctgt acagccacag cagccgtatt
    19381 atgcgccagc agcggaacaa cctgtgcaac aaccttacta cgctcctgcg gcggaacaac
    19441 ctgttcagca gccgtattat gcaactgcgg cggaacagtc tgcgcaacag ccgtattacg
    19501 cccctgcgcc agaacaaacg gcggcaggta acgcctggca agccgaagag cagcaatcca
    19561 cttttgctcc acagtctaca taccagactg agcaaactta tcagcagcca gccgctcagg
    19621 agccgttgta ccaacagccg caacccgttg aacagcaccc tgttgtggag cctgagcccg
    19681 ttgtagaaga gacaaaaccc gcgcgtccgc cgctttacta ttttgaagaa gtggaagaga
    19741 agcgtgcccg tgaacgtgaa caacttgcgg cctggtatca accgattcct gaaccggtta
    19801 aagaaccaga accgatcaaa tcttcgctga aagcaccttc tgttgcagca gtacctccag
    19861 tggaagccgc tgccgctgtt tctccgctgg catctggcgt gaaaaaagcg acactggcga
    19921 cgggggctgc cgcaaccgtt gccgcgccag tcttcagtct ggcaaatagc gctggaccgc
    19981 gtccgcaggt caaagagggg attggcccgc agttgccacg accgaaacgt atccgcgtgc
    20041 caactcgtcg tgaactggcg tcttacggta ttaagctgcc ctcacagcgt gcggcagaag
    20101 aaaaagcccg tgaagctcag cgcaatcagt acgattccgg cgatcagtat aacgatgatg
    20161 aaatcgatgc gatgcagcag gatgaactgg cacgtcagtt cgcccagaca cagcagcaac
    20221 gctatggcga acagtatcaa catgatgtgc ccgtgaacgc agaagatgca gatgctgcgg
    20281 cagaggctga actggctcgt cagtttgctc aaactcaaca acaacgttat tccggcgaac
    20341 aacctgctgg ggcgaatccg ttcactctgg atgattttga attttcgcca atgaaagcgt
    20401 tgctggatga tggtccacac gaaccgctgt ttacgccaat tgttgaacct gtacagcagc
    20461 cgcaacaacc gattgcaccg cagcaacagt atcagcagcc gcagcaacca gttgcgccgc
    20521 agccgcagta tcagcagccg caacagccgg ttgcaccgca gcagcaatat cagcagccac
    20581 aacaaccggt tgcaccgcag ccgcaatatc agcagccgca acagccggtt gcaccacagc
    20641 cgcaatatca gcagccgcaa cagccagttg cgccgcagcc gcatgatacc ctgcttcatc
    20701 cgctgttgat gcgcaatggt gacagccgtc cgttgcataa accgacgacg ccgctgcctt
    20761 ctctggattt gctgacgccg ccgccgagcg aagtggagcc ggtagatacc tttgcgcttg
    20821 aacaaatggc gcgcctggtg gaagcgcgtc tggctgattt ccgtattaaa gccgatgtcg
    20881 tcaattattc tccggggccg gttatcactc gctttgaatt gaacctggca ccgggagtaa
    20941 aagcggcgcg tatttctaac ttgtcacgtg accttgcccg ttcactttcg acggtggcgg
    21001 tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg gctggagtta ccgaataaaa
    21061 aacgacaaac cgtttatctg cgcgaagttt tggataacgc caaattccgc gataatccgt
    21121 cgccattaac cgtggtgctg ggtaaagata tcgccggtga gccggtggtt gccgatctgg
    21181 cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc cggtaagtct gtcggtgtga
    21241 acgcgatgat cctgagcatg ctttataaag cacagccaga agatgtgcgt ttcatcatga
    21301 tcgacccgaa aatgctggag ctttcggttt atgaaggcat cccgcatctg ttaacggaag
    21361 tcgttactga tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac gagatggagc
    21421 gtcgttataa actgatgtct gcgctgggtg tgcgtaatct ggcgggttat aacgaaaaaa
    21481 ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc gtactggaag ccgggtgata
    21541 gtatggatgc gcatcatccg gtgctgaaaa aagaaccgta catagtggtg ttggtggacg
    21601 aatttgccga cctgatgatg acggtaggta aaaaagtgga agagctgata gcacgtctgg
    21661 cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc aacgcagcgt ccatcggttg
    21721 atgttattac tggtctgatt aaagcgaata ttccgacccg tatcgccttt accgtatcca
    21781 gtaagattga ctcacgtacc attcttgatc aggctggcgc ggaatcactg ctgggtatgg
    21841 gggatatgct ctactctggg ccgaactcca cgttgccggt acgtgtccat ggtgcttttg
    21901 tacgcgatca ggaagttcat gccgtggtgc aggactggaa agcgcgtggt cgcccacagt
    21961 atgttgacgg catcacctcc gacagcgaaa gcgaaggtgg tgcgggtggt ttcgatggcg
    22021 ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa aaacgcaaag
    22081 cgtcaatttc aggcgtacag cgtcagttcc gcattggtta taaccgtgca gcgcgtatta
    22141 tcgaacagat ggaagcgcaa gggattgtca gcgaacaggg gcacaacggt aatcgtgaag
    22201 tgctggcccc accgccgttt gactaattaa tgcatcgtag gccggataag gcgcggtagc
    22261 gtcgcatccg gcactctaac aactgaaaat tcagtatttt cttctttcct caagctgatt
    22321 attagcctgg aatagagagt agagggaact cccgatcggg agtgacgtaa tttgaggaat
    22381 aatgatgaaa aaaattgcca tcacctgtgc attactctca agcttagtag caagcagcgt
    22441 ttgggctgat gccgcaagcg atcttaaaag ccgcctggat aaagtcagca acttccacgc
    22501 cagcttcaca caaaaagtga ctgacggtag cggcgcggcg gtgcaggaag gccagggcga
    22561 tctgtgggtg aaacgtccaa atttattcaa ctggcatatg actcaacctg atgaaagcat
    22621 tctggtttct gacggtaaaa cactgtggtt ctataacccg ttcgttgagc aagctacggc
    22681 aacctggctg aaagatgcca ccggtaatac gccgtttatg ctgattgccc gcaaccagtc
    22741 cagcgactgg cagcagtaca atatcaaaca gaatggcgat gactttgtcc tgacgccgaa
    22801 agccagcaat ggcaatctga agcagttcac cattaacgtg ggacgtgatg gcacaatcca
    22861 tcagtttagc gcggtggagc aggacgatca gcgcagcagt tatcagctga aatcccagca
    22921 aaatggggct gtggatgcag cgaaatttac cttcaccccg ccgcaaggcg tcacggtaga
    22981 tgatcaacgt aagtagaggc acctgagtga gcaatctgtc gctcgatttt tcggataata
    23041 cttttcaacc tctggccgcg cgtatgcggc cagaaaattt agcacagtat atcggccagc
    23101 aacatttgct ggctgcgggg aagccgttgc cgcgcgctat cgaagccggg catttgcatt
    23161 ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac cctcgctgaa gtgattgccc
    23221 gctatgcgaa cgctgatgtg gaacgtattt ctgctgtcac ctctggcgtg aaagagattc
    23281 gcgaggcgat tgagcgcgct cggcaaaacc gcaatgcagg tcgccgcact attctttttg
    23341 ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc atttctgcca catattgaag
    23401 acggcaccat cacttttatt ggcgcaacca ctgaaaaccc gtcgtttgaa cttaactcgg
    23461 cactgctttc ccgtgcccgt gtctatctgc tgaaatccct gagtacagag gatattgagc
    23521 aggtgcttac tcaggcgatg gaagacaaaa cccgtggcta tggtggtcag gatattgttc
    23581 tgccagatga aacacgacgt gccattgctg aactggtgaa tggcgacgcg cgccgggcgt
    23641 taaatacgct ggaaatgatg gcggatatgg ccgaagtcga tgatagcggt aagcgggtcc
    23701 tgaagcctga attactgacc gaaatcgccg gtgaacgtag cgcccgcttt gataacaaag
    23761 gagatcgctt ttacgatctg atttccgcac tgcataagtc ggtacgtggt agcgcacccg
    23821 atgcggcgct gtactggtat gcgcgaatta ttaccgctgg tggcgatccg ttatatgtcg
    23881 cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa tgccgatcca cgggcgatgc
    23941 aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg cccggcggaa ggtgaacgcg
    24001 ccattgctca ggcgattgtt tacctggctt gcgcgccaaa aagcaacgct gtctacactg
    24061 cgtttaaagc cgcgctggcg gatgcacgcg aacgtccgga ttatgacgtg ccggttcatt
    24121 tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta cgggcaggaa tatcgttacg
    24181 ctcatgatga agcaaacgct tatgctgccg gtgaggttta tttcccgccg gaaatagcac
    24241 aaacacgcta ttatttcccg acaaacaggg gccttgaagg caagattggc gaaaagctcg
    24301 cctggctggc tgaacaggat caaaatagcc ccataaaacg ctaccgttaa tgttatcgtt
    24361 gcggtaatgt tgttactgta tccctgtggt cgcaggctgt ggccacatct cccatttaat
    24421 tcgataagca caggataagc atgctcgatc ccaatctgct gcgtaatgag ccagacgcag
    24481 tcgctgaaaa actggcacgc cggggcttta agctggatgt agataagctg ggcgctcttg
    24541 aagagcgtcg taaagtattg caggtcaaaa cggaaaacct gcaagcggag cgtaactccc
    24601 gatcgaaatc cattggccag gcgaaagcgc gcggggaaga tatcgagcct ttacgtctgg
    24661 aagtgaacaa actgggcgaa gagctggatg cagcaaaagc cgagctggat gctttacagg
    24721 ctgaaattcg cgatatcgcg ctaaccatcc ctaacctgcc tgcagatgaa gtgccggtag
    24781 gtaaagacga aaatgacaac gttgaagtca gccgctgggg caccccgcgt gagtttgact
    24841 ttgaagttcg tgaccatgtg acgctgggtg aaatgcactc tggcctcgac tttgcagccg
    24901 cagttaagtt gaccggttcc cgctttgtgg taatgaaagg gcagattgct cgcatgcacc
    24961 gcgcactgtc gcagtttatg ctggatctgc ataccgaaca gcatggctac agtgagaact
    25021 atgttccgta cctggttaac caggacacgc tgtacggtac gggtcagctg ccgaaatttg
    25081 ctggcgatct gttccatact cgtccgctgg aagaagaagc agacaccagt aactatgcgc
    25141 tgatcccaac ggcagaagtt ccgctgacca acctggtgcg cggtgaaatc atcgatgaag
    25201 atgatctgcc aattaagatg accgcccaca ccccatgctt ccgttctgaa gccggctcat
    25261 atggtcgtga cacccgtggt ctgatccgta tgcaccagtt cgacaaagtt gaaatggtgc
    25321 agatcgtgcg cccggaagac tcaatggcgg cgctggaaga gatgactggt catgcagaaa
    25381 aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat cctttgcact ggcgacatgg
    25441 gctttggcgc ttgcaaaact tacgacctgg aagtatggat cccggcacag aacacctacc
    25501 gtgagatctc ttcctgctcc aacgtttggg atttccaggc acgtcgtatg caggcacgtt
    25561 gccgcagcaa gtcggacaag aaaacccgtc tggttcatac cctgaacggt tctggtctgg
    25621 ctgttggtcg tacgctggtt gcagtaatgg aaaactatca gcaggctgat ggtcgtattg
    25681 aagtaccaga agttctgcgt ccgtatatga acggactgga atatattggc taatacccaa
    25741 tttttctgaa tctaaaaagc gcctgcgggc gctttttttg tctccctttg ataccgaaca
    25801 ataattactc ctcacttaca cgtaatacta ctttcgagtg aaaatctacc tatctctttg
    25861 attttcaaat tattcgatgt atacaagcct atatagcgaa ctgctataga aataattaca
    25921 caatacggtt tgttactgga atcaatcgtg agcaagcttg agtgagccat tatgaaaacg
    25981 aaaatccctg atgcggtatt ggctgctgag gtgagtcgcc gtggtttggt aaaaacgaca
    26041 gcgatcggcg gcctggcaat ggcaagcagc gcgttaacat taccttttag tcggattgcg
    26101 cacgctgttg atagtgccct tccggcaaaa tcagacgaaa aggttatctg gagcgcctgt
    26161 acagttaact gtggtagtcg ctgcccgcta cgtatgcacg ttgtggacgg tgaaattaaa
    26221 tatgttgaaa cggataatac cggcgatgac aactatgacg gcctgcacca ggtgcgcgcc
    26281 tgtctgcgtg gtcgttccat gcgtcgccgt gtctacaatc cggaccgcct gaaatatccg
    26341 atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc gcattagctg ggaagaagcc
    26401 tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag agtacggcaa cgagtctatc
    26461 tatctgaact atggtaccgg aacgctgggc ggcaccatga cccgctcctg gccgccggga
    26521 aataccctgg tcgctcgcct gatgaactgc tgcggcggtt atctgaacca ttacggtgac
    26581 tactcttccg cgcaaattgc ggaaggtttg aactatacct acggcggctg ggcagatggt
    26641 aacagcccgt cggatatcga aaacagtaaa ctggtagtgc tgtttggtaa taaccctggc
    26701 gaaacgcgaa tgagtggcgg tggggtgact tactatcttg aacaggcgcg ccagaaatct
    26761 aatgctcgca tgatcatcat cgatccgcgc tataccgaca ccggtgccgg gcgcgaagat
    26821 gagtggatcc ctattcgtcc gggaacagat gccgcactgg ttaacggtct ggcgtatgtc
    26881 atgatcaccg aaaacctggt ggatcaggca ttcctcgata aatattgcgt tggctacgac
    26941 gagaaaacac tgccagccag tgcgccgaaa aatggtcact ataaagctta tattctgggt
    27001 gaagggccag atggcgtggc taaaacgccg gaatgggcct cgcaaatcac tggtgttccg
    27061 gcagacaaaa tcatcaaact ggctcgtgaa atcggtagta ccaaaccggc gtttatcagc
    27121 cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg caacccgtgc tatctcgatg
    27181 ctggcgattc tgaccggtaa cgttggtatt aacggaggca acagcggcgc gcgtgaaggt
    27241 tcatacagct taccgtttgt ccgtatgccg accctcgaaa acccgatcca gaccagcatt
    27301 tcgatgttta tgtggaccga tgccattgaa cgtggcccgg aaatgacagc gctgcgtgat
    27361 ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa tgatctggaa ctatgccggt
    27421 aactgcctga ttaatcagca ttctgaaatc aaccgtaccc atgaaatcct tcaggatgat
    27481 aagaagtgcg agctgattgt ggttatcgac tgccacatga cctcatcggc gaaatatgct
    27541 gacatcctgc tgcctgactg caccgcttca gaacagatgg actttgcact ggatgcatcc
    27601 tgcgggaata tgtcttacgt gattttcaac gatcaggtga ttaaaccgcg ctttgaatgt
    27661 aagaccatct atgaaatgac cagcgaactg gcaaaacgtc ttggcgttga gcaacagttt
    27721 actgagggcc gtacccagga agagtggatg cggcatctgt atgcccagtc gcgggaagcg
    27781 attcctgaac tgccaacgtt tgaagagttc cgtaagcagg ggatctttaa aaagcgcgac
    27841 ccacaagggc atcacgttgc ttataaagct ttccgtgaag atccgcaggc aaatccactg
    27901 accacgccat cggggaaaat tgagatttat tcgcaggcgc tggctgacat tgccgctacc
    27961 tgggaattgc cagaaggtga tgtgatcgat ccactgccga tctacacgcc gggctttgaa
    28021 agttatcagg atccgctgaa caaacagtat ccgctgcagc ttacgggttt ccactataaa
    28081 tctcgcgttc actcaactta cggcaacgtt gatgtgctga aagcagcttg ccgtcaggaa
    28141 atgtggatca acccgcttga tgcgcaaaaa cgcggtatca acaacggcga taaagtcagg
    28201 atctttaacg atcgtggtga ggttcatatt gaggcgaaag tgacgccacg aatgatgccg
    28261 ggtgtggtcg cactgggtga aggtgcctgg tatgacccgg atacaaaacg tgtcgataag
    28321 ggcggttgta tcaacgtact gaccactcaa cgtccgtctc ctctcgctaa ggggaatccg
    28381 tcacatacaa acctggttca ggttgaaaag gtgtaaggag taaccgatga caacccagta
    28441 tggatttttt attgattcca gccgttgcac cggttgcaaa acctgcgagc tggcttgtaa
    28501 agactacaaa gatttgacgc cagaagtcag tttccgccgc atttatgaat atgctggcgg
    28561 cgactggcag gaagataacg gtgtctggca ccagaacgtg tttgcctact atctgtcgat
    28621 ttcatgtaac cactgcgaag atccggcttg tactaaagtc tgcccgagcg gtgcgatgca
    28681 taaacgtgaa gatggttttg ttgtggtcga tgaagatgtg tgcattggct gccgttactg
    28741 ccatatggct tgcccgtacg gcgcaccgca atataacgaa acgaaaggcc atatgaccaa
    28801 atgcgatggt tgttatgacc gtgttgcgga ggggaaaaag ccgatctgtg ttgaatcttg
    28861 cccgctgcgg gcgctggatt tcggccctat cgacgagctg cgtaaaaaac atggcgatct
    28921 ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa ccgaatattg tgatcaaacc
    28981 caatgccaac agccgcccga ccggggatac cactggctat ctggcaaacc cgaaggaggt
    29041 gtgagatggg aagtggatgg catgaatggc cgctgatgat cttcacggtc ttcgggcaat
    29101 gtgtagcggg tggttttatc gttctggctt tggcgctgct caaaggcgac ctgcgagcag
    29161 aagcccagca gcgtgttatt gcctgcatgt ttggtttatg ggtgctgatg ggcattggct
    29221 ttatcgcctc tatgcttcat cttggttcac cgatgcgcgc ctttaactcg ctcaaccggg
    29281 taggggcttc agcactcagt aacgaaatcg ccagcggttc gatctttttt gctgttggtg
    29341 gcatcggctg gttgctggca atgctgaaaa agctgtcacc ggcattgcgt acgctgtggc
    29401 tgatagtgac gatggttctt ggcgtcatct ttgtctggat gatggtgcgt gtgtataaca
    29461 gcattgatac cgttccgacc tggtacagca tctggacgcc gatgggcttc ttcctgacga
    29521 tgttcatggg cggcccgtta ctgggttacc tgttgctgag tctggctggc gtcgatggct
    29581 gggcgatgcg tctgctgcca gccatttctg tactggcact ggtagtgagt ggcgtgatgt
    29641 cagtgatgca gggcgcagag ctggcgacta ttcatagctc tgtgcagcag gccgcagcgc
    29701 tggtgccgga ctatggcgcg ctgatgtcct ggcggatcgt gcttttggct gttgccctgt
    29761 gcttgtggat tgcaccacag ctaaaaggtt atcagcctgc ggttccgcta ctttctgtat
    29821 cattcattct gcttctggca ggggaattaa tcggtcgtgg cgtattctat ggtttgcata
    29881 tgaccgtggg gatggctgtc gcaagttaat cagtaacaac cggggtttcg gccccggttc
    29941 gcttttattt ctgcttcgtt aacgtgtcat aactggtcat caagttacga taatcaggaa
    30001 tgtggttaga gaacaacgtc gccagtcctt caatatcatt acgccagtcg cgatgcagct
    30061 cgcatgccac gccgaaccat gtcatcaatt gcgcaccagc ttgcgacatg cgatcccatg
    30121 ccgaatgccg ggtaatttca ttaaaggtcc ctgacgcgtc agtcacgaca aagacgtcaa
    30181 acccttcttc aatggctgaa agcgccggga atgccacgca aacttcggtt accacaccgg
    30241 caataattaa ctgtttttta cctgtcgctt tgacagcttt tacaaaatct tcgttatccc
    30301 aggcgttaat atttcccggg cgagcaatgt aaggtgcatc ggggaattgt gctttcagtt
    30361 ctggaactaa tgggccgtta ggaccggttt caaaactggt ggtaagaata gttggtaagt
    30421 tgaaatactt agccaggtca cccagcgcca gcacgttatt tttaaactta tcgggttcga
    30481 tatcccgtac aagggaaagt aaaccagcct ggtgatcaac aagcaaaacg gcagcatcat
    30541 ttttatcaag acgaacatac ggtttggtca tcctcttctc ctttcgagag tcagtggtac
    30601 gtctgaaatc ggtcagacgc gatgcattgc tctgaaagca tagacgggaa atatgagttt
    30661 gctgtgacca tgaaattttt cgactgaacg aagcgtatga aatttgtgtt agatcaataa
    30721 aaacaatcag atgagattaa tcgtctctcc agcattagcg ttatgaatga gtaatataaa
    30781 tcgataatac ctccgctgcg atatgcgcgt attttcaccc attgacaatg tttttggcgg
    30841 tggcatgatg cgcatgaaat ttgaacttcc tcacggtttt aattcatgtc cacgtatacc
    30901 cggcctgtca tgcttttgct gtctggcctg cttttgttga ctctggcgat cgcagtgtta
    30961 aatacactcg tgccgctttg gctcgcccag gaacacatgt ccacatggca ggtaggcgtt
    31021 gtcagctcat cctattttac cggcaacctg gtcggtacat tgctgacagg gtatgtcatt
    31081 aagcgcattg gctttaaccg cagctattat ctggcctcct tcatttttgc cgctggctgt
    31141 gccggccttg gcctgatgat tggattctgg agctggttgg cttggcgttt tgtcgccggt
    31201 gtcggttgtg ccatgatttg ggtggttgtt gagagcgcgc tgatgtgcag tgggacgtca
    31261 cgtaaccgtg ggcgtttgct tgctgcgtat atgatggttt attacgtggg aacgttttta
    31321 ggccagttac tggtcagcaa agtttcaacc gagctgatgt ccgtattgcc gtgggttaca
    31381 ggtttgacgt tggcagggat cttaccgctg ttgtttacgc gtgtgctgaa tcagcaggct
    31441 gaaaaccatg attcgacgtc aattacgtca atgctaaaac tccgtcaggc gcggcttggc
    31501 gtgaatggct gcattatctc aggaatcgtg ctgggatctc tatatggcct gatgccgctg
    31561 tacctcaatc acaaaggggt gagcaatgcc agcattggtt tctggatggc ggtactggtc
    31621 agtgcgggta tcctcggaca atggccgatc ggacgtctgg cggataagtt tggtcgactg
    31681 ctggtgttgc gtgttcaggt ctttgtcgtc attctcggca gtatcgcgat gcttagccag
    31741 gcggcgatgg ccccagcgtt attcatcctc ggtgccgctg gctttacgct atatccggtg
    31801 gcgatggcct gggcttgtga gaaagttgaa catcatcaac tggtggcgat gaaccaggcc
    31861 ttactgttga gctataccgt gggaagtctg cttggcccgt catttaccgc tatgctaatg
    31921 cagaatttct ccgataattt attgtttatc atgatcgcca gcgtatcgtt tatctatttg
    31981 ctgatgctgc ttcgcaacgc cggtcatacg ccgaaacccg ttgctcacgt gtaaatgaat
    32041 tcaagcagag tgtgaactta ctgtttcaca ctctgctttt ttgtttcttc tatctgactt
    32101 gctttattcc aaattttatt cgtttaaaaa taaaatgtgc agcaggttat aattttgcat
    32161 ttcgctattt ccgcacttct tatttgccgc gcataatccc tcgttttaca gatgcccctt
    32221 taattttggc gaaggatttg tctatggctg ggaatgttca ggaaaaacag ctgcgatggt
    32281 acaacattgc gctgatgtct tttatcactg tctggggttt tggcaatgtt gttaataact
    32341 acgccaacca ggggctggtg gttgtttttt catgggtgtt tatctttgcg ctctatttca
    32401 caccttatgc gctaattgtt ggtcagttag gctcgacctt caaagatggg aagggcgggg
    32461 tcagtacctg gattaaacac acgatgggac ccggactggc ttacctcgcc gcgtggacct
    32521 actgggtagt gcatattccc tatctggcac aaaaacccca ggcaatactg attgcgctcg
    32581 gttgggcgat gaaaggcgac ggttcgttaa tcaaagaata ttcagtcgta gcgttacagg
    32641 ggttaacgct ggtgctgttt atcttcttta tgtgggttgc ttcacgcggt atgaaatcgc
    32701 tgaaaatcgt cggttctgtg gcagggattg cgatgtttgt tatgtcgctc ctgtatgtgg
    32761 cgatggcggt aaccgcgcct gcaattactg aagtgcatat tgcgaccaca aacattacct
    32821 gggaaacgtt cattcctcat atcgacttta cctacattac cactatttca atgctggttt
    32881 tcgcggttgg cggagcagag aagatttctc cttacgttaa tcaaacgcgc aacccaggaa
    32941 aagaatttcc aaaagggatg ttatgcctgg cggtgatggt tgcggtttgt gccattctgg
    33001 gttcactggc gatggggatg atgtttgact cgcgtaatat cccggatgac ttaatgacta
    33061 acggtcagta ttacgccttt cagaagctgg gcgagtatta caacatgggt aatactttaa
    33121 tggtgattta cgccattgcg aataccctgg gacaagtagc tgcgctggta ttctcgattg
    33181 atgccccgct taaagtgcta ttaggcgatg ctgacagcaa atatattcca gccagtttat
    33241 gtcgtaccaa cgcttctggt acgcccgtta atggctattt tctgaccctg gtgctggtgg
    33301 cgatcctgat aatgctaccg actctcggca ttggtgatat gaacaatctc tataaatggc
    33361 tgttgaatct taactcggta gtgatgccgc tgcgttattt atgggtattt gttgcattta
    33421 ttgcagttgt tcgcctggcg cagaaatata agccggaata tgtctttatt cgtaataagc
    33481 cgctggcaat gaccgtcgga atttggtgtt ttgcctttac cgcttttgcc tgcctgactg
    33541 gtattttccc gaaaatggaa gccttcaccg cagagtggac attccagttg gcgctgaatg
    33601 ttgcaacgcc gtttgtgctg gtaggattag ggctgatatt cccgctgctg gcgcgtaaag
    33661 cgaatagcaa ataattattg tggtcggtga tgctgactct ggcagatctg acccgttttt
    33721 acataatcga gaaatgcgcg caagccgcag gacatatact ggcggtttgg gtaatacatt
    33781 tggaagctgt gctgtaaggg cgttagaaat agagctctga attatttatg cagcgcggaa
    33841 taaaaaagcg gcaactcaaa aaagttgccg ctttacgggg aaattagaac attaccttat
    33901 gaccgtactg ctcaagaatg cctttcacgc gttccatggt ctctttcttc ggtggtttaa
    33961 caccatcaag tttgtattct tcacccatcg ccacccattt gtgtttgccc agttcgtggt
    34021 aggggagaag ctcgattttc tcaacgttgc ccatatcacg ggtaaattca ccgaggcgat
    34081 gcgctgaatc gtcatcgtca gaccagcctg ggacaacaac gtagcggatc cacaccttca
    34141 catttttgtt cgccagatat ttggcgaact ccagcgtgcg gtggttggaa actccaacca
    34201 gattttggtg gatctcgtcg ttcatctgtt tgagatcgag cattaccagg tcggttactt
    34261 ccagcagttc atcaatcacc ggatcgtaac gacgaacaaa accgttggtg tccagacagg
    34321 tatgaatgcc ttcttttttg caggcgcgga accagtcacg aacaaactca gcttgcagga
    34381 ttgcttcacc gccggatgcg gtaacgccgc cgccggaagc gttcataaag tggcgatagg
    34441 tcaccacctc tctcattaaa tcttcaacgg taacttcttt accgccatgc gtgtcccagg
    34501 tgtcgcggtt atgacaatac aggcagcgca tcaggcagcc ctggaaaaag gtgataaagc
    34561 gaatacccgg accgtctacg gttccacagg attcaaagga gtgaatgcga ccaataactg
    34621 acattgcggt gtttctccag atgtggccca tctgaggccg tgttggtgcg cagctcgaag
    34681 gctacgtcga gtctgttttg gcagtcacct taaagtatag atagctgaca aaaaaggctc
    34741 tcgtgctaaa aaaggcccca ctttcgtgga gcctttattg tacgcttttt actgtacgat
    34801 ttcagtcaaa tctaattaca tagattgagt gaaggtacga gtaataacgt cctgctgctg
    34861 ttctttagtc agcgagttga aacgtactgc gtagccagat acacggatgg tcagctgcgg
    34921 atatttttcc gggttttcca tcgcgtcgag cagcatttca cggttcatca cgttaacgtt
    34981 caggtgctga ccaccttcga tggacgcttc gtggtggaag taaccatcca tcagaccagc
    35041 caggttggtc ttacgaactt cgtcgtcttt acccagtgcg ttcggaacga tagagaaggt
    35101 gtaggagata ccatctttag cgtaagcaaa cggcagttta gcaacggaag tcagagaggc
    35161 tactgcacct ttctggtcac gaccgtgcat cgggttagca cccggtccga acggcgcgcc
    35221 agcacgacga ccgtctgggg tgttaccagt tttcttacca tacacaacgt tagaagtgat
    35281 ggtcagaaca gactgagtcg ggatagcgtc acggtaggtg tgcagtttct gaattttctt
    35341 catgaaacgt tctaccaggt caacagccag gtcatctaca cgcggatcgt tgttaccaaa
    35401 ctgcgggtat tcgccttcga tttcgaagtc gatagccaga ccgtcttcgt cacgaatcgg
    35461 tttaactttc gcatatttga ttgcagacag ggagtcagca gcaacggaca gaccagcgat
    35521 accacacgcc atggtgcgga taacgtcacg gtcgtgcagc gccatcagag aggcttcgta
    35581 gctgtacttg tcgtgcatgt agtggatgat gttcagtgca gtgatgtact gtttagccag
    35641 ccagtccatg aagtgatcca tgcgctccat cacttcatcg tagttcagga gatcgccttt
    35701 gatcggctca gacttcggac caacctgcat tttcagtttt tcgtcaacgc cgccgttgat
    35761 tgcgtacagc atggttttcg ccaggtttgc acgcgcaccg aagaactgca tttgtttacc
    35821 aacgatcatc gggcttacgc agcaagcgat agcgtagtca tcgttgttga agtccggacg
    35881 catcaggtca tcgttctcat actgcagaga agaggtgtcg atggacactt tagcagcgaa
    35941 tttcttgaag ttcagcggca gtttttcgga ccacagaatg gtcatgttcg gttccggaga
    36001 cggacccatg gtgtacaggg tgttcaggaa acggaagctg tttttggtaa ccagggtacg
    36061 accgtcgagg cccataccac cgatagattc ggttgcccag atcgggtcgc cagagaacag
    36121 ttcatcgtat tccggagtac gcaggaagcg aaccatacgc agtttcatga ccaggtggtc
    36181 aaccatttcc tgcgcttctt gttcggtgat cttgccagct ttcaggtcac gttcgatgta
    36241 cacatccagg aaggtggagg tacgaccgaa ggacattgca gcaccgttct gagacttaac
    36301 agcagccagg tagccgaagt aagtccactg gatagcttcc tgagcgttgg tagccggacc
    36361 agagatgtcg tagccgtatt tcgcagccat ttctttcatc tgacccagag cgcggtgctg
    36421 ttcagcgatt tcttcgcgca gacggatagt ctgttccagg tttacgccgt tttccagatc
    36481 agcctgcaga gaggtgaact gagcgtattt gtctttcatc agatagtcga taccgtacag
    36541 cgcaacgcga cggtagtcac cgatgatacg accacggcca taagcatctg gcagaccggt
    36601 cagaacaccg gatttacggc aacgcaggat gtccggagtg taaacgtcga acacgccctg
    36661 gttgtgagtt ttacggtatt cagtgaagat ttttttgatc atcgggtcca gttcgcggtt
    36721 gtacgctttg caggaacctt cgatcatttt gataccaccg aacgggataa gagcacgttt
    36781 cagcggagct tcagtctgca gaccaacaac tttttccaac gctttgttga tgtagccagc
    36841 gtcgtgagag gtgatggtgg aagcaacagc ggtgtcaaag tcaactggcg cgtgagtgcg
    36901 gttttccagt ttaacgcctt ccattacttt gtcccacagg gtggtggtcg cttcagtagc
    36961 gccagccagg aaggactcgt caccctcgta cggagtgtag tttttctgaa tgaagtcacg
    37021 gacgtttact tcattctgcc agtcaccttt ggtaaaacct tcccaggctg tggctaactt
    37081 ttcattaagc tcggacatgt aacacctacc ttcttaagtg gattttttat ttactgcgta
    37141 cttcgacaac cattaatggt gatcgttttc acgcagataa atgacccagt atgtcaaccc
    37201 aaccaacaaa ccaccgccga taatattacc gatcgtaacc ggaatcaggt tatcagtgat
    37261 gaagttcatc acggtcaggt gagaaaaatt ttccggtgca gaaccgacag cggtccagaa
    37321 ttccggagat gcgaagtcgc ggattacaat acccatcggg atcataaaca tgtttgcgat
    37381 actgtgctca aaaccgctgg caacaaacat cgcgaccggc agcaccataa tgaacgcttt
    37441 atccatcagg ctgcggccag aataactcat ccatactgcc agacatacca tcaggtttgc
    37501 caggatacca agacagacgg cctcaataaa agtatggtgc actttgtggt cggcggtttg
    37561 taggacgttt agtccccatt gaccattcgc ggtcatatac tcgccggaaa gccacattaa
    37621 aagtacaaac agcagtgcgc cgaccaggtt gccaaaatag acatttagcc agtttttcgc
    37681 taactgaccc caggtgatgc gcccactcgc cttagcaaca acaatcaaca ctgtggaagt
    37741 aaagagatcg gctccgcaga caacacaaag aatcagcccc agagagaagc aaatgccgcc
    37801 aaccagtttt gccatgccga agggcattgt tcctgtgcca gtggttgctg tgatatagaa
    37861 gacgaatgcg attgagatga aaacaccggc ggtaatcgcc agatagaaag tcttaagcgg
    37921 atgtttcgtt gctttataga cacctgcctc ttcggccact ttggccattg ctgcaggaag
    37981 taaaagatca aaagggttgt cagctttcac actaactctc tctttattaa gtcggcgacg
    38041 agatactaac aaagcattat agatgagaaa ttgatataga t
//
LOCUS       DAZKJR010000030        37857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKJR010000030 DAZKJR010000000
VERSION     DAZKJR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.030"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..221
                     /locus_tag="ACHOWS_001581"
     CDS             <1..221
                     /locus_tag="ACHOWS_001581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047172.1"
                     /translation="LIMVCDFKFVLWINCEAELIREEKQKKTPQSAAFGIVCIFLAGC
                     GVNASPGLVELKTTRLLSPRYHAALRCG"
     gene            complement(172..1836)
                     /gene="glnS"
                     /locus_tag="ACHOWS_001582"
     CDS             complement(172..1836)
                     /gene="glnS"
                     /locus_tag="ACHOWS_001582"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFQ0047173.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGSFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSCEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2039..3985)
                     /gene="nagE"
                     /locus_tag="ACHOWS_001583"
     CDS             complement(2039..3985)
                     /gene="nagE"
                     /locus_tag="ACHOWS_001583"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFQ0047174.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDVMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4318..5118
                     /gene="nagB"
                     /locus_tag="ACHOWS_001584"
     CDS             4318..5118
                     /gene="nagB"
                     /locus_tag="ACHOWS_001584"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0047175.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5178..6326
                     /gene="nagA"
                     /locus_tag="ACHOWS_001585"
     CDS             5178..6326
                     /gene="nagA"
                     /locus_tag="ACHOWS_001585"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0047176.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            6335..7555
                     /gene="nagC"
                     /locus_tag="ACHOWS_001586"
                     /gene_synonym="nagR"
     CDS             6335..7555
                     /gene="nagC"
                     /locus_tag="ACHOWS_001586"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFQ0047177.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7603..8355
                     /gene="nagD"
                     /locus_tag="ACHOWS_001587"
     CDS             7603..8355
                     /gene="nagD"
                     /locus_tag="ACHOWS_001587"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFQ0047178.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8611..10275
                     /gene="asnB"
                     /locus_tag="ACHOWS_001588"
     CDS             8611..10275
                     /gene="asnB"
                     /locus_tag="ACHOWS_001588"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFQ0047179.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10656..10732
                     /locus_tag="ACHOWS_001589"
     tRNA            10656..10732
                     /locus_tag="ACHOWS_001589"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10690..10692,aa:Met,seq:cat)
     gene            10741..10825
                     /locus_tag="ACHOWS_001590"
     tRNA            10741..10825
                     /locus_tag="ACHOWS_001590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10775..10777,aa:Leu,seq:tag)
     gene            10849..10923
                     /locus_tag="ACHOWS_001591"
     tRNA            10849..10923
                     /locus_tag="ACHOWS_001591"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10881..10883,aa:Gln,seq:ttg)
     gene            10958..11032
                     /locus_tag="ACHOWS_001592"
     tRNA            10958..11032
                     /locus_tag="ACHOWS_001592"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10990..10992,aa:Gln,seq:ttg)
     gene            11048..11124
                     /locus_tag="ACHOWS_001593"
     tRNA            11048..11124
                     /locus_tag="ACHOWS_001593"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11082..11084,aa:Met,seq:cat)
     gene            11173..11247
                     /locus_tag="ACHOWS_001594"
     tRNA            11173..11247
                     /locus_tag="ACHOWS_001594"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11205..11207,aa:Gln,seq:ctg)
     gene            11285..11359
                     /locus_tag="ACHOWS_001595"
     tRNA            11285..11359
                     /locus_tag="ACHOWS_001595"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11317..11319,aa:Gln,seq:ctg)
     gene            complement(11513..12688)
                     /gene="ubiF"
                     /locus_tag="ACHOWS_001596"
     CDS             complement(11513..12688)
                     /gene="ubiF"
                     /locus_tag="ACHOWS_001596"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFQ0047180.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            12834..14258
                     /gene="miaB"
                     /locus_tag="ACHOWS_001597"
     CDS             12834..14258
                     /gene="miaB"
                     /locus_tag="ACHOWS_001597"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFQ0047181.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14411..15451
                     /gene="ybeZ"
                     /locus_tag="ACHOWS_001598"
     CDS             14411..15451
                     /gene="ybeZ"
                     /locus_tag="ACHOWS_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0047182.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15448..15915
                     /gene="ybeY"
                     /locus_tag="ACHOWS_001599"
     CDS             15448..15915
                     /gene="ybeY"
                     /locus_tag="ACHOWS_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0047183.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            16005..16883
                     /gene="corC"
                     /locus_tag="ACHOWS_001600"
     CDS             16005..16883
                     /gene="corC"
                     /locus_tag="ACHOWS_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFQ0047184.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16908..18446
                     /gene="lnt"
                     /locus_tag="ACHOWS_001601"
     CDS             16908..18446
                     /gene="lnt"
                     /locus_tag="ACHOWS_001601"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFQ0047185.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKH"
     gene            complement(18524..20401)
                     /locus_tag="ACHOWS_001602"
     CDS             complement(18524..20401)
                     /locus_tag="ACHOWS_001602"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0047186.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGAVERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLSSGAGTHHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVTDIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPTGDARGIVSAMGFRPKNGEIIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            20856..21764
                     /gene="gltI"
                     /locus_tag="ACHOWS_001603"
     CDS             20856..21764
                     /gene="gltI"
                     /locus_tag="ACHOWS_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFQ0047187.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21934..22674
                     /gene="gltJ"
                     /locus_tag="ACHOWS_001604"
     CDS             21934..22674
                     /gene="gltJ"
                     /locus_tag="ACHOWS_001604"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFQ0047188.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            22674..23348
                     /gene="gltK"
                     /locus_tag="ACHOWS_001605"
     CDS             22674..23348
                     /gene="gltK"
                     /locus_tag="ACHOWS_001605"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFQ0047189.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23348..24073
                     /gene="gltL"
                     /locus_tag="ACHOWS_001606"
     CDS             23348..24073
                     /gene="gltL"
                     /locus_tag="ACHOWS_001606"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFQ0047190.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24191..25126
                     /gene="rihA"
                     /locus_tag="ACHOWS_001607"
     CDS             24191..25126
                     /gene="rihA"
                     /locus_tag="ACHOWS_001607"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFQ0047191.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(25171..25653)
                     /gene="ybeL"
                     /locus_tag="ACHOWS_001608"
     CDS             complement(25171..25653)
                     /gene="ybeL"
                     /locus_tag="ACHOWS_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFQ0047192.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25888..28470
                     /gene="leuS"
                     /locus_tag="ACHOWS_001609"
     CDS             25888..28470
                     /gene="leuS"
                     /locus_tag="ACHOWS_001609"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0047193.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28485..29066
                     /gene="lptE"
                     /locus_tag="ACHOWS_001610"
     CDS             28485..29066
                     /gene="lptE"
                     /locus_tag="ACHOWS_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFQ0047194.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            29066..30097
                     /gene="holA"
                     /locus_tag="ACHOWS_001611"
     CDS             29066..30097
                     /gene="holA"
                     /locus_tag="ACHOWS_001611"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0047195.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSHPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            30099..30740
                     /gene="nadD"
                     /locus_tag="ACHOWS_001612"
     CDS             30099..30740
                     /gene="nadD"
                     /locus_tag="ACHOWS_001612"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0047196.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30764..31375
                     /gene="cobC"
                     /locus_tag="ACHOWS_001613"
     CDS             30764..31375
                     /gene="cobC"
                     /locus_tag="ACHOWS_001613"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFQ0047197.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            31635..31952
                     /gene="rsfS"
                     /locus_tag="ACHOWS_001614"
     CDS             31635..31952
                     /gene="rsfS"
                     /locus_tag="ACHOWS_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0047198.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31956..32423
                     /gene="rlmH"
                     /locus_tag="ACHOWS_001615"
     CDS             31956..32423
                     /gene="rlmH"
                     /locus_tag="ACHOWS_001615"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0047199.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            32454..34355
                     /gene="mrdA"
                     /locus_tag="ACHOWS_001616"
     CDS             32454..34355
                     /gene="mrdA"
                     /locus_tag="ACHOWS_001616"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFQ0047200.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            34358..35470
                     /gene="mrdB"
                     /locus_tag="ACHOWS_001617"
                     /gene_synonym="rodA"
     CDS             34358..35470
                     /gene="mrdB"
                     /locus_tag="ACHOWS_001617"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFQ0047201.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35481..36569
                     /gene="rlpA"
                     /locus_tag="ACHOWS_001618"
     CDS             35481..36569
                     /gene="rlpA"
                     /locus_tag="ACHOWS_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFQ0047202.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGLYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36709..>37857
                     /gene="dacA"
                     /locus_tag="ACHOWS_001619"
     CDS             36709..>37857
                     /gene="dacA"
                     /locus_tag="ACHOWS_001619"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFQ0047203.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPE"
BASE COUNT         9252 a         9859 c         9767 g         8979 t
ORIGIN      
        1 atttaattat ggtttgtgat tttaaatttg ttttatggat aaattgcgaa gcggaattaa
       61 ttcgcgaaga aaagcagaaa aaaacgccgc agagcgcggc gtttgggatt gtttgcatat
      121 ttcttgccgg atgtggcgta aatgcctcac ccggcctggt agaacttaaa actactcgcc
      181 tactttcgcc caggtatcac gcagccctac ggtgcggtta aataccggtt tttcggcagt
      241 ggaatggcgg ctgtcgaggc agaaataacc ttcacgctca aactggaatg ctttacccgc
      301 taccgcgtct ttcagcgacg gttcagcgaa accctgtttg atcaccagcg attccgggtt
      361 aatcaccgac aggaaatcat ccgcagcacc tgggttaggc acgctgaaca gacggtcata
      421 caaacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttcac
      481 tttacgacca tctgccggat ctttgcttaa ggtgtcggcg tcataagtac agaagatggt
      541 ggtgatatta ccttcggcat ctttctcgac gcgttctgcc ttaatcacgt aagcattacg
      601 cagacgcact tctttaccca acaccagacg tttgtactgc ttgttagctt cttcgcggaa
      661 atcggcgcga tcgatccaaa tctcacagct aaacggcacc tgacggctac ccatttccgg
      721 tttgttcgga tggttcggca tggtgaccat ttcgccttcg ccctggtagt tttcgataac
      781 cagtttcacc ggatcgataa ccgccattgc gcgcggggca ttttcgttga gatcttcacg
      841 gatgcaggat tccagtgacg ccatctcaat ggtgttgtcc tgcttggtca caccaatgcg
      901 tttgcagaac tcacgaatag aagccgcagt gtaaccacga cgacgcagac cagaaatggt
      961 cggcatacgc gggtcatccc agccttcaac gtgcttatcg gtcaccagca ggttcagctt
     1021 acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcgggtg
     1081 aacaggaatg gtgatgttgt ccagtaccca gtcgtacaga cggcggttgt cctggaactc
     1141 aagcgtacac agagagtgcg taataccttc cagcgcatcg ctgatgcagt gggtgaagtc
     1201 gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatgcg
     1261 gtacagcacc ggatcgcgca ttacgataaa tggcgacgcc atgtcgattt tcgcacgcag
     1321 gcaggcttta ccttcttcaa aactaccggt acgcattttt tcgaacagcg ccaggttctc
     1381 ttcaacgctg cggtcgcggt acgggctgtt tttacccggc tgcgtcaggg tgccgcggta
     1441 ttcgcggatc tgttccggtg tcagttcatc aacgtacgcc aggcctttat tgatcagttc
     1501 gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca
     1561 gtgaaaacct aaccactcaa cgtcgttttt gatcgactca acatactcga tatcttcttt
     1621 taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc
     1681 gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg
     1741 gaaacgggtg tgtactgtgg tgtgcttacc actggccaga tcttcatcga tgatctgacg
     1801 aataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca
     1861 acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag ttagtgaaaa
     1921 tcgaaaatct cactgagatt atggggatga ttgatgaaaa aaaaagcggc agagaagatc
     1981 tccaccgctt gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt
     2041 actttttgat ttcatacagc ggtgtttgac ccgccacaac atggccctga gctttaatga
     2101 tcaggccgct gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc gagcgggcgt
     2161 tagcgttcag gtaatccaga tccatttcca gaatcggttg tcctgcgctt acctgcgcgc
     2221 cctcttccac cagacgttta aagcctttac cttccagcgc tacggtgtcg atacccatat
     2281 ggacgacgat ctccgcgcct ttttcggttt ccagacagaa cgcgtggttg gtgttgaaga
     2341 ttttcacgat tgtccctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca
     2401 caccgtcacc caccgctttg ctggcgaatg cttcgtcagg aacctgatcc agtgccacaa
     2461 catcaccggt aatcggcgat accagctccg cgatagacac cgcgtttggt acagcctgcg
     2521 gttttgctac aggcgcggca gttgcaggcg cagtttcagc tgacgcagcg gctaccggac
     2581 cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg cccacaatca
     2641 cctgaatagt ctgtttgttc agttttacta ccccagaagc ccccagacgt ttacacatca
     2701 catcgttgac gcgagctgag tcaaccacgg taaggcgcag acgagtaata caggcgtcaa
     2761 tggctttcag gttgtcagta ccgccaaccg cagcaatata gttggttgcc agttgattga
     2821 gaccttcttc cgtgttgctg ttggcttctt cagtaacgat ctcgtcttct ttatcttcac
     2881 ggcccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag
     2941 cgaagaagac aacacccatc accagcagca tccagacgtt ctggctggcg gccggcaggt
     3001 tatacatcaa cgcgtagtcg atagcccccg cagagaagga gaagcccgcg tggataccca
     3061 gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg
     3121 gcgcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa
     3181 cagcaacaga aagcagcata ccgccaacca tcggacgacg ctctttcggt gctgcaaagt
     3241 acatcgccag cgccgcaccc ggcagaccga acatcatgat cgggaagaag ccggacatga
     3301 acatccccgc ggtgccgtca ccggcataga agcggttaat gtcgccgtgg aaaaccgtac
     3361 ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagt acctgatgca
     3421 gaccggttgg gatcagcaga cggttgatga aaccaaagat accggaaccc agcgcgcccg
     3481 cagaaacgat ccactcgccg cctgcatgga tagcgtgctg taccggcggc cagacgtaac
     3541 caaaaatggc cgccagcacc aggcagaaga agccggtggc aatcggcaca aagcgtttgc
     3601 cgccgaagaa gctcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcac
     3661 caccgaccag accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg
     3721 tcaccatcgc tttggttaac acaaagtaac ctaccgcacc cgccagtgcc gccgcacctg
     3781 cgctgtcttt cgaccagctg gatgccacgc cgatggcgaa gattaatgcg aggttatcaa
     3841 aaatcgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc
     3901 gcagcaacag cgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctacctagtc
     3961 gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt
     4021 taaagccgta tttttatttt gctgcaaatt gtactgccga tgttctgtaa tcagattgtt
     4081 agatcatctg ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa acgcgtgtct
     4141 tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc taaaccagaa
     4201 aacttatttt atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt
     4261 tacgcttaaa gatgcctaat ccgccaacgg cttacatttt acttattgag gtgaataatg
     4321 agactgatcc ccctgactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc
     4381 aatcgtatca atgcgttcaa accgactgcc gatcgtccgt ttgtactggg cctgccgact
     4441 ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc
     4501 agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat
     4561 ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca
     4621 gaaaatatca accttctcaa cggcaacgcc ccggatatcg acgccgaatg ccgccagtat
     4681 gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac
     4741 ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc
     4801 ctgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt taatcaggtg
     4861 ccaaaatatg ccctgactgt cggtgttggt acattgctgg atgccgaaga agtgatgatt
     4921 ctggtgctgg gtagccagaa agcactggca ctgcaggccg ccgttgaagg ttgcgtgaac
     4981 cacatgtgga ccatcagctg tctgcaactg catccaaaag cgatcatggt gtgcgatgaa
     5041 ccttccacca tggagctgaa agttaagact ttaagatatt tcaatgaatt agaagcagaa
     5101 aatatcaaag gtctgtaatt attatccctg ccctggctcc ttgctcaggg caatattttt
     5161 taaaatcggg ggtcagaatg tatgcattaa cccagggccg gatctttacc ggccacgaat
     5221 ttcttgatga ccacgcggtt gttatcgctg atggcctgat taaaagcgtc tgtccggtag
     5281 cggaactgcc gccagagatc gaacaacgtt cactgaacgg ggccattctc tcccccggtt
     5341 ttatcgatgt gcagttaaac ggctgcggcg gcgtgcagtt taacgatact gctgaagcag
     5401 tcagcgtaga aacgctggaa atcatgcaga aagccaatga gaaatcaggc tgtactaact
     5461 atttgccgac gcttatcacc accagcgacg agctgatgaa acagggcgtg cgcgttatgc
     5521 gcgagtacct ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa ggtccgtggc
     5581 tgaatctggt aaaaaaaggc acccataatc cgaattttgt gcgtaagcct gatgccgcgc
     5641 tggtcgattt cctgtgtgag aacgccgacg tgattaccaa agtgactctg gcaccggaaa
     5701 tggttccagc ggaagtcatc agcaaactgg caaatgccgg gattgtggtt tctgctggtc
     5761 actccaacgc gacgttgaaa gaagcgaaag ccggtttccg cgcggggatt acctttgcca
     5821 cccatctgta caacgcgatg ccgtatatta ccggtcgtga accgggcctg gcgggcgcga
     5881 tcctcgacga agctgacatt tattgcggta ttattgctga tggcctgcat gttgattacg
     5941 ccaacattcg taacgctaag cgtctgaaag gcgacaaatt gtgtctggtt accgatgcca
     6001 ccgcgccagc aggtgccaac attgaacagt tcatttttgc gggtaaaaca atatactacc
     6061 gtaatggact ttgtgtggat gagaacggta cgttaagcgg ttcatcctta accatgattg
     6121 aaggcgtgcg taatctggtc gaacattgtg gtatcgcact ggatgaagtg ctgcgtatgg
     6181 cgacgctcta tccggcacgt gcgattggcg ttgagaaacg tctcggcacg ctcgccgcag
     6241 gtaaagtagc caacctgact gcattcacac ctgattttaa aatcaccagg accatcgtta
     6301 acggtaacga ggtcgtaact caataagaga aagtatgaca ccaggcggac aagctcagat
     6361 aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc tgattgacca
     6421 gtacgggcca atctcgcgga ttcagattgc cgagcaaagt cagcttgccc ccgccagcgt
     6481 aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg atcagcaggc
     6541 ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt tccacgcaat
     6601 cggcgtacgg cttggtcgtc acgacgccac catcaccttg tttgatctca gcagcaaagt
     6661 gctggcagaa gaacattacc cgctgccgga acgtacccag cagacgctgg aacatgccct
     6721 gctgaatgcc attgctcagt ttattgatag ctaccagcgc aagctacgcg agctgatcgc
     6781 gatttcggtg atcctgccag ggcttgtaga cccggacagc ggcaaaattc attatatgcc
     6841 gcatattcag gtagaaaact gggggctggt tgaagcactg gaagagcgtt ttaaagtgac
     6901 ctgttttgtc ggccacgata tccgcagtct ggcgctggca gagcactact tcggtgcaag
     6961 tcaggattgc gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat
     7021 ctctaacggg cgaattttta tcgggcgcaa cggcaacgtc ggtgaaattg gccatattca
     7081 ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt ggctgcctgg aaactatcgc
     7141 cgccaatgct gccattgaac aacgggtgtt gaatctgtta aagcagggct accagagccg
     7201 tgtgccactg gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag
     7261 cctggcctcg gaagtgattg aatatgtcgg tcgtcatctg ggtaaaacca tcgccattgc
     7321 tatcaactta tttaatccgc aaaaaattgt tattgctgga gaaatcaccg aagccgataa
     7381 agtgctgctc cctgctattg aaagctgcat taatacccag gcgctgaagg cgtttcgtac
     7441 taatctaccg gtggtacgtt ctgagttgga tcaccgctca gcaatcggcg cttttgcgct
     7501 ggtaaaacgc gccatgctca acggtatttt gctccagcat ttgctggaaa attaatgtgc
     7561 ttttatagtg gcgcttattg ttgtcaatat tctgggtagt ccatgaccat taaaaatgta
     7621 atttgcgata tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa
     7681 tttttgcacg ggattatgga taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg
     7741 cagaccgggc aagatctggc gaatcgcttt gccactgccg gtgtcgatgt accggatagc
     7801 gtgttttata cctccgcgat ggcgaccgcc gatttcctgc gtcgccagga aggcaagaaa
     7861 gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt acaaagccgg tttcactatt
     7921 accgatgtga accctgattt tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg
     7981 atgcataaag cagcctattt cgtcgctaac ggcgcacgct ttatcgccac caacccggac
     8041 acccacgggc gcggttttta tcccgcctgt ggcgcgttgt gtgcagggat cgagaaaatc
     8101 tccgggcgca aaccgttcta tgttggaaag ccgagtccgt ggatcatccg cgcggcatta
     8161 aacaaaatgc aggcgcattc ggaagaaacg gtgattgtcg gcgataactt gcgtaccgat
     8221 attctggcgg gcttccaggc cggtctggag acgattctgg tgctttctgg tgtttcgtca
     8281 ctcgacgata tcgacagtat gccgttccgc ccaagttgga tttacccatc ggtcgctgaa
     8341 atcgacgtta tctgaatagc aaccacgcct cagggcgtgg tttcttattt ttacccatca
     8401 ttaaaatcac cccattcaat aaaacccgtc aaaaattatc gatccatcaa taaatatacc
     8461 caaaagccct cccctttttc atcaatcagc aatcgccatt acgtttttta tttttccact
     8521 gccaaatcgc ttgcgcaccg tgctgctttg cggcattttt ttaacaagta aacacaacac
     8581 gcagtaaaca ccaggttaac ggagaaggtt atgtgttcaa tttttggcgt attcgatatc
     8641 aaaacagacg cagttgagct gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat
     8701 cgtggcccgg actggtccgg tatttatgcc agcgataacg ccatcctcgc ccacgaacgt
     8761 ctgtcaattg ttgacgttaa cgcgggggcg caacctctct acaaccaaca aaaaactcat
     8821 gtgctggcag taaacggtga aatctacaac caccaggcat tgcgcgctga atatggcgat
     8881 cgttaccagt tccagaccgg gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa
     8941 gggccggaat ttctcgacga cttgcagggc atgtttgcct ttgccctgta cgacagcgaa
     9001 aaagatgcat atctgattgg tcgcgaccat ctggggatca tcccactgta tatgggctat
     9061 gacgaacacg gtcagctgta tgtggcctca gaaatgaaag ccctcgtgcc agtttgccgc
     9121 acgattaaag agtttccggc ggggagctat ttgtggagtc aggacggcga aatccgttct
     9181 tattatcatc gcgactggtt cgactacgat gcggtgaaag ataacgtgac cgacaaaaac
     9241 gagctgcgtc aggcactgga agattccgtt aaaagccatc tgatgtctga tgtgccttac
     9301 ggtgtgctgc tttctggtgg tctggattcc tcaattattt ccgctatcac caagaaatac
     9361 gccgcccgtc gcgtggaaga tcaggaacgc tctgaagcct ggtggccgca gttgcactcc
     9421 tttgctgtag gtctgccggg ctcaccggat ctgaaagcag cccaggaagt ggcaaaccat
     9481 ctgggcacgg tgcatcacga aattcacttc actgtacagg aaggtctgga tgccatccgc
     9541 gacgtgattt accacatcga aacttatgat gtgaccacta ttcgcgcttc aacaccgatg
     9601 tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt
     9661 tctgacgaag tgtttggcgg ttatctatac ttccacaaag cgccgaacgc taaagagttg
     9721 catgaagaga cggtgcgtaa actgctggcc ctgcatatgt atgactgcgc gcgtgccaac
     9781 aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt
     9841 gatgtggcga tgcgcattaa cccacaggat aaaatgtgcg gtaacggcaa aatggaaaaa
     9901 cacatcctgc gtgaatgttt tgaagcgtat ctgcctgcaa gcgtggcctg gcggcaaaaa
     9961 gagcagttct ccgatggcgt cggttacagt tggatcgaca ccctgaaaga agtggcagcg
    10021 cagcaggtgt ctgatcaaca gctggaaact gcccgcttcc gcttcccgta caacacgccg
    10081 acctcaaaag aagcgtatct gtaccgggag atctttgaag aattgttccc gcttccgagc
    10141 gccgctgagt gcgtgccggg cggtccttcc gtcgcttgtt cttccgctaa agcgattgag
    10201 tgggatgaag cgttcaagaa aatggacgat ccgtctggtc gcgcggttgg cgttcaccag
    10261 tcggcatata aataagacgt gtaatattgc cccggagaaa tccggggcaa tttcagttac
    10321 tggggcgctt gttgattctg atatgcagta aggaaatgtt ccgggtttgc cacatcgctg
    10381 tttgacaact cattactgta ggccagtatt tcatagatta atttcccacc actattgttg
    10441 aattccgaat ttcatcctgt atgacaaaag aatggctgta gggtggggaa aaaacacaaa
    10501 gtaaacaata attgacgaat atagcgccat gctgttcgca acctaaccaa acagtcactt
    10561 tcgagcaatt ttccttgaaa aagaggttga cgctgcaagg ctctatacgc ataatgcgcc
    10621 ccgcaacgcc gataaggtat cgcgaaaaaa aagatggcta cgtagctcag ttggttagag
    10681 cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac catctttttt
    10741 gcgggagtgg cgaaattggt agacgcacca gatttaggtt ctggcgccgc aaggtgtgcg
    10801 agttcaagtc tcgcctcccg caccattcac cagaaagcgt tgtacggatg gggtatcgcc
    10861 aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag
    10921 ccatattctt cgaataagcg gttcaccgcc cggttattgg ggtatcgcca agcggtaagg
    10981 caccggtttt tgataccggc attccctggt tcgaatccag gtaccccagc catcgaagaa
    11041 acaatctggc tacgtagctc agttggttag agcacatcac tcataatgat ggggtcacag
    11101 gttcgaatcc cgtcgtagcc accaaattct gaatgtgtcg aatatgttcg gcaaattcaa
    11161 aaaccaattt gttggggtat cgccaagcgg taaggcaccg gattctgatt ccggcattcc
    11221 gaggttcgaa tcctcgtacc ccagccaatt tattcaagac gcttaccttg taagtgcacc
    11281 cagttggggt atcgccaagc ggtaaggcac cggattctga ttccggcatt ccgaggttcg
    11341 aatcctcgta ccccagccac attaaaaaag ctcgcctcgg cgagcttttt gcttttctgc
    11401 gtatattcaa tgccgaatgc gatgttgact cgtcttatcc ttcaaagtcg gatgcgacgc
    11461 tgccgcgtct tatccgacct acggttggca tgcatccagc aatgttgtga ggctacaacc
    11521 ctaacgcata tttcagcgcc ttacgtttca acacgccagc acgctccgcc gccatcaaac
    11581 caagattacg cacaaaacgc agcggtggca gattattgct gaatccggca taaaacagat
    11641 ccataccact ttgcatcata aagttatccg ccatacgccg catctggtaa cgcttgagca
    11701 caggataact ggcccacgct tcgccgtagc tgcgggcgtt caccagaacg tcaatcaggg
    11761 catcgacatc acgataacca agattcaccc cctgccccgc cagcggatgg atggtatgcg
    11821 cggcatcgcc caccagagca agccctggtt gcacatactg caacgcatgg cgacgtatca
    11881 gcggaaacgc accagcggca agcggtgtca cgtaacccag acgcgacggg aaatgcttcg
    11941 cgatttccgc ctgtagctgc gccatattca tattctgcaa ctggcgaatg cgcgccggag
    12001 agtcatacca caccagcgat gcccagttat caaacaacgg cagaaacgca cgcggtccgt
    12061 ccggggtaaa ttgctgccag gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca
    12121 acatacacga ctgcgcatac tgccaggcat gaacgccaat tcccgccatc tgtcgcacct
    12181 gcgaatttgc gccgtcggca ccaatcacca gcttcgcgag gatcgtttca ccgcccttca
    12241 gttccagctc ctgaagatca ttatggcgat gcagtgcaat cagcgaggtt ggcacacgta
    12301 acgttacttt cggatgcgct tccagcgcct gccacaacgc ctgttgcagg acagtgtttt
    12361 ccaccatgta gccaagcaac ggtagcttaa gttcagcggc gtcaaacacc acatgcgccg
    12421 tttcccactc ccacgtttcc agtctgcggt aaggatggca acgcatagcc tgtactgcat
    12481 cccagacccc taacccttta agcaatgata ccgaagccgc actaatcgcc gagatccgca
    12541 cgtccggctg gctgtcagcg acaaacggtg caggttgggc atgttcgata accattaccg
    12601 aaaatccgtg ctgagccagt cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa
    12661 tggcaatttc cgttggttga tttgtcatgg tcaatcatcc tgttagcaat atgctttaag
    12721 tttacaggat ttttaccccc aagggctgat aacgctcata ctggtcacaa cggcagcaaa
    12781 gcattacact atgcgccctg cattcctggc tactatttcg caagagcaag tcgatgacca
    12841 aaaaactcca tattaaaacc tggggctgtc agatgaacga gtacgattca tcgaagatgg
    12901 ccgatctgct ggatgccacc cacggctatc aactgaccga cgtggcggaa gaagcggatg
    12961 tgctgctgct gaacacctgc tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt
    13021 tgggtcgctg gaaactgtta aaagagaaga atccagacct gattatcggc gtcggcggct
    13081 gcgtggcatc gcaagaaggc gagcacattc gccagcgcgc ccactatgtc gatattattt
    13141 ttgggccgca aacgctgcac cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca
    13201 gcccggttgt agatatcagc ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc
    13261 gcgccgaagg gccgaccgcg tttgtctcca tcatggaagg ctgcaataaa tattgcacct
    13321 actgcgtagt gccttacacc cgtggtgaag aggtaagccg tccgtccgac gatattctgt
    13381 ttgagattgc ccagcttgct gctcagggcg tgcgtgaagt taacctgctc ggccagaacg
    13441 tgaacgcctg gcgcggtgag aactacgacg gcaccaccgg atcgtttgcc gatctgctgc
    13501 gtctggttgc ggcgatcgac gggatcgatc gtattcgctt taccaccagc catccgatcg
    13561 aattcaccga cgatatcatc gaagtgtacc gcgacacgcc ggagctggtg agcttcctgc
    13621 atctgccggt acagagcggt tccgatcgca ttctgaacct gatgggacgt acccatacag
    13681 cgctggagta caaagcgatc atccgtaagc tgcgtgcggc gcgtccggat attcagatca
    13741 gttctgactt catcgttggc ttccctggcg aaaccaccga agacttcgag aaaacgatga
    13801 agctgattgc cgacgtcaat ttcgacatga gctacagctt tatcttctcc gctcgtccag
    13861 gtacaccagc cgccgatatg gttgatgatg ttccggaaga agagaagaag cagcgcctgt
    13921 atattctaca agaacgtatc aatcagcaag caatggcatg gagccgccgg atgctcggta
    13981 ccacccagcg tattctggta gaaggtacat cgcgtaagag catcatggag ctttccggcc
    14041 gtactgaaaa taaccgcgtc gtcaacttcg aaggtacgcc ggatatgatc ggtaaattcg
    14101 tcgatgtaga aattaccgac gtctacccga actctctgcg cggtaaagtg gtgcgaactg
    14161 aagatgaaat gggtctgcgc gtggcagaaa caccggaatc ggtgattgcc cgtacccgca
    14221 aagaaaacga ccttggcgtg ggttattatc agccgtaatt ctcaggcccg ccgttccggt
    14281 gggccttgtt ttttctctct ttatccccat ctttatcgca atgcttgcgc ctttttcctg
    14341 tggcgtgaat aatttcctta taggccggtg aatgttcagc ttccggcggc atacagataa
    14401 gaggaacggt ttgaacatag acactcgcga aatcaccctg gagccagcag acaatgcgcg
    14461 tctgttgagc ctgtgcggcc cgtttgatga caacatcaaa caactcgaac gccgtctcgg
    14521 catcgaaatc aatcgccgcg ataaccactt taaactgacc ggccgtccga tttgcgtcac
    14581 cgctgcggca gacattctgc gtagcctgta tgtcgatact gccccgatgc gcggtcagat
    14641 tcaggatatc gaaccggaac agatccacct tgcgattaaa gaagcgcggg tactggagca
    14701 aagcgcggag agcgtgccgg agtacggcaa agcggtcaat atcaaaacca aacgcggcgt
    14761 aattaagccg cgcacgccaa accaggcgca gtacatcgcc aatattctcg accatgacat
    14821 taccttcggc gttggcccgg cgggtacggg taaaacctac ctggcagtgg ctgcggcagt
    14881 tgatgccctg gagcgtcagg aaattcgccg tattctgctg actcgtccgg cagtagaagc
    14941 cggtgagaaa ctgggcttcc tgcctggcga tttaagccag aaagtagacc cgtatctgcg
    15001 cccgctgtac gacgcgctgt ttgaaatgct gggctttgag aaagtcgaga aactgattga
    15061 gcgcaacgtt attgaagtcg caccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc
    15121 gtttatcatt cttgatgaga gccagaacac caccatcgaa cagatgaaga tgttcctgac
    15181 ccgtatcggt tttaactcaa aagcggttat caccggcgac gtcacacaga tcgacctgcc
    15241 gcgtaatacc aagtctggtt tgcgtcatgc catcgaagta ctggctgatg tcgaagagat
    15301 cagctttaac ttcttccaca gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt
    15361 taacgcctat gaagcctggg aagaagccga acaaaaacgt aaagcggcgc tggcagcaga
    15421 acgcaagcgc gaagaacagg aacaaaaatg agtcaggtga tcctcgattt acaactggca
    15481 tgtgaagata attccgggtt accggaagag agccagtttc agacatggct gaatgcggtg
    15541 atcccgcagt ttcaggaaga atcggaagtg actattcgcg tggtcgatac cgccgaaagc
    15601 cacagtctga atctgaccta tcgcggtaag gataagccga ccaacgtgct ctccttcccg
    15661 tttgaagtac cgcctggcat ggaaatgtcg ctactgggcg atctggttat ctgccgtcag
    15721 gtggttgaga aggaagctca ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg
    15781 gtggtgcacg gcagtctgca tttgttaggt tacgatcaca tcgaagatga cgaagcagaa
    15841 gaaatggaag ccatcgaaac agagattatg cttgctctgg gctatgagga tccgtacatt
    15901 gccgagaaag aataatttgc caggcatttg actggcagac gtccccgccg ttggtgatta
    15961 acgacggcgt tttgattaac taacatgaga acccatacga cgccatgagc gacgacaatt
    16021 cacacagtag tgacacgata agcaacaaga agggattttt ctccctgtta ctcagccaac
    16081 ttttccacgg tgaaccgaaa aaccgtgacg aactgctggc gctgatccgt gattccgggc
    16141 agaacgacct tatcgacgaa gatacgcgcg atatgctcga aggggtgatg gacatcgcag
    16201 accaacgcgt tcgcgacatc atgatccccc gctcccagat gattaccctg aaacgcaacc
    16261 agacgctgga cgaatgcctt gatgtcatca tcgagtccgc ccactcacgt ttcccggtga
    16321 ttagcgaaga caaagatcac attgaaggga ttctgatggc gaaagacttg ctgccgttta
    16381 tgcgcagcga tgctgaagcc ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc
    16441 ctgaaagtaa gcgcgtagac cggatgctga aagagtttcg ctctcagcgt taccacatgg
    16501 cgatcgtcat tgacgaattc ggtggggttt ccggtctggt gaccattgaa gacatcctgg
    16561 aactgattgt tggtgagatt gaagacgagt atgacgaaga agatgatatc gacttccgtc
    16621 agctgagtcg tcatacctgg accgtgcgcg cactggcttc tattgaagac ttcaacgaag
    16681 cgttcggcac ccactttagc gatgaagagg ttgacactat cggtggtctg gtgatgcagg
    16741 catttgggca tcttccggcg cgtggcgaaa ccatcgacat cgacggttac cagttcaaag
    16801 tggcgatggc cgacagtcgg cgtattattc aggttcatgt caaaatcccg gatgactcac
    16861 cccagccgaa gctggatgaa taaaaccgaa actggataga taactacatg gcttttgcct
    16921 cattaattga acgccagcgc attcgcctgc tgctggcgtt attattcggt gcctgcggaa
    16981 cgctggcctt ctctccttac gacgtctggc ctgcggcgat tatttcgctg atggggcttc
    17041 aggcgttgac ctttaaccgc cgtccactcc agtctgccgc tattggcttt tgctggggat
    17101 ttggcctctt tggcagcggt attaactggg tctatgtcag catcgcgacc tttggcggaa
    17161 tgcctggccc ggttaacatc ttcctggtgg tactgctggc ggcgtatttg tcgctgtata
    17221 ccggactgtt tgctggcgtg ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg
    17281 cgattgccgc ccctgctctc tggcaagtga ccgagtttct gcgcggttgg gtactaacag
    17341 gcttcccgtg gttacagttc ggctatagcc agattgatgg cccgttaaaa gggctggcac
    17401 cgataatggg cgtggaagcc attaacttcc tgctgatgat ggttagcggc ctgctggcac
    17461 tggcgttggt caaacgcaac tggcgtccgc tggtggtggc cgtcgtgctg tttgcccttc
    17521 ccttcccgct gcgttacatc cagtggttta ccccgcaacc ggaaaaaact attcaggttt
    17581 cgatggttca gggcgatatt ccgcaatcgc tgaaatggga cggagaccag ctacttaata
    17641 cgctgaagat atactacaac gcaacggcac cgctgatggg caaatcatcg ttgattatct
    17701 ggccggagtc ggcgataacc gatctggaaa ttaatcagca accgttcctc aaagctctgg
    17761 acggtgagtt gcgcgataaa gggagctcgc tggtcaccgg gattgtcgac gcgcgtctca
    17821 ataaacagaa ccgctacgat acctacaaca ccatcatcac actgggtaaa ggcgcaccgt
    17881 acagctatga atcagccgat cgctataaca aaaaccatct ggtgccgttt ggcgagtttg
    17941 tcccactgga gtcgatactg cgtccgttag caccgttctt tgatttgccg atgtcttcat
    18001 tcagccgcgg accatatatt cagccaccgc tgtcgttgaa tggcattcaa cttactgcgg
    18061 ccatttgcta cgaaatcatt ctcggcgagc aagtgcgcga taacttccgc ccggataccg
    18121 actatctgct gactatctcc aacgatgcgt ggtttggtaa gtctattggt ccatggcagc
    18181 acttccagat ggcgcgaatg cgtgcgctgg agctggcgcg cccactgttg cgcagcacca
    18241 acaacggcat tacggcggtg attggcccgc agggtgagat tcaggcgatg atcccgcagt
    18301 tcacccgcga ggtgttaacc actaacgtga cgccgactac cggactcaca ccgtacgcac
    18361 gtaccggcaa ctggccgctg tgggtgctga cggcactgtt tggttttgca gctgtgctga
    18421 tgagtctgcg agcacgtaaa cattaatgtg cctgatgtat ccggcatgaa caaaacatac
    18481 tttctcattg agttgtgggg catgattatc atgccctttt ttcttacggt tcagggatac
    18541 aagccacatc gccatattgg ggatgccgaa taattgcctg cggatcaagc gagcgcgtcc
    18601 tgtccatttg gattaacctg ttcttcgcca taacaaatat ctttctacca tccgctgaaa
    18661 aacgaaggtg attaacgtag ctggcaggat aaacgccaat aatctgcccg gcattaatat
    18721 tgagcaggat gacataacct tgcaggccat atgacaccaa cgctaaatat tgaccgtccg
    18781 ggctaatacc ctcaacggaa taaacgctcc tgctacacca ttgcgtcata atgctggctt
    18841 gttgatcccc tacgcttata cgtgaaatcc cctggctatc ggcaaaaata atctcgccat
    18901 tttttggtcg gaatcccatc gcagaaacga taccacgcgc atcacctgtg ggaaagtctt
    18961 ctttctgatg cgtgatggta ttgatgcgta caatgtcgcc tctgaccata ccgggtatca
    19021 acaatgtttt ttcatccggg gaaagtgcca taacccaggc gcctggttta ccgctcgtcc
    19081 ccgtcccgtc gtatttctcc agcgggatat ccgtcacatg ctgataagtc accagatcaa
    19141 tggctgacac actattactg gtgcgattca tcacccaggc ttttgtgcca tcacgcgaga
    19201 ggataaggct gtcaggatgg gcgccggtgg taatcgactg aataatctcc cccgtcgcca
    19261 cgttaatgac cgatactgca ttcgcttcaa atgaagtcgc ataaatcaac ttttcatcca
    19321 ggcttaatgc cagcgatctg acgccctgat ctttacagcc accacaatgt ttgactgact
    19381 tttctttcgc tatgggcgct gtaaaggtat gcaggatttt cttttgtccg aggtcgaaaa
    19441 caacaatgct gttggtgttt tcaattgcgg catacaacgt attaccatcc cgactgatcg
    19501 ccatatcatg gatatcggca atctcagcga gtaaatcacc actggcctgc tcacgactta
    19561 ccggagcgga taatgtttcc ctgagggcgt tgcgctcctc cactaactgc tgcttttttt
    19621 gtcgtcgctc ttgttcaatc tcctgcggat aaaacgcttc acgcaggctt tgcctgacct
    19681 gtaaaactga ttcatcaatc tgctgctgca caaaccagat agtcccggtc agaagaagag
    19741 tgaccgcaac aataatcccg ccttccgtaa cgagccgatt ttgcccaact aaacgcgcac
    19801 tcagccaacc caacgcgccc cccgcaatca ggccgccaat gtggcaagcg ttatctattc
    19861 ctgcttgccg ggcaccatac aacagtgtca gcgccaccat acccaacaga gaaaatactc
    19921 gccgctggtt tttatggtgt gtacccgcac ctgaagaaag ctgtgttgct atcgcagcgc
    19981 ccgctattcc cataattgcc ccggatgccc caacgctgat gactatctgc tcactttcgc
    20041 gtaacgcata acaggcgctg atcagcccac caaagatgcc ggagaataac cagataatca
    20101 ggagtcgcca tttccccaga atacgttcca ccgctccccc caccaccagg agtgcaaaca
    20161 tattcatcag caaatgagaa acactgctgt gcagaaagac actgctcaat aagcgccagg
    20221 attcacccga caacgtgaga gaagcaacgt tagcaccgag atggataagt gcggattgat
    20281 cgacttcttc ataactcatc cattgtgcga ggtaacaaaa tatcccaaca cagagcgcaa
    20341 aaaggataag ggttattgcg ggtaattgaa catttaacgg cttaaccgaa gatgcagaca
    20401 taaggtgatt cttccttgaa aagacaggta aaaagcattt ttcccgtgtg cagttatcat
    20461 cccggcaaac gtcccatgcc gaaaagataa ccctgtccga aatatgacta tttcagccta
    20521 acattacaat cgtaattaat tcacctataa gtattttttg gcatgtaagt tgctttattt
    20581 actgtgtaag caatgaaatc agccgcgacg caaccgggtt gcaccaccac aaatcagcgg
    20641 tagcaccaaa aaggtgcgat ttaagaattt tgcttcggaa tggtgcggcg catctcgcaa
    20701 aaacaaacaa taaaacagca atatctttac atttagccaa actaaatgtt acctaacaag
    20761 cacaacactg cacaataaag ttgcagatga taacaacaca aacactcaca acgggtatcc
    20821 atgcgttctt aacgcagaag ataaaggagt tggatatgca attacgtaaa cctgccacag
    20881 caatcctggc cctggcgctt tccgcaggac tggcacaggc agatgacgcc gccccggcag
    20941 caggcagtac gctggacaaa atcgccaaaa acggcgtgat tgtcgtcggt caccgtgaat
    21001 cttcagtacc tttctcttat tacgacaatc agcaaaaagt ggtgggctac tcgcaggatt
    21061 actccaacgc cattgttgaa gcagtgaaaa agaaactcaa caaaccagac ttacaggtga
    21121 aattgattcc gatcacctca caaaaccgta ttccactgct gcaaaacggc actttcgatt
    21181 ttgaatgcgg ttctaccacc aacaatgccg aacgccaaaa acaggcggct ttctctgaca
    21241 ctattttcgt ggtcggtacg cgcctgctga ccaaaaaggg cggcgatatc aaagattttg
    21301 ccgacctgaa aggcaaagcc gtagtcgtca cttccggcac cacctctgaa gttttgctca
    21361 acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg
    21421 actctttccg taccctggaa agcggtcgtg ccgttgcctt tatgatggat gatgctctgc
    21481 tggccggtga acgtgcgaaa gcgaagaaac cagacaactg ggaaatcgtc ggcaagccgc
    21541 agtctcagga agcctatggt tgcatgttgc gtaaagacga tccgcagttc aaaaagctga
    21601 tggatgatac catcgctcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataagt
    21661 ggttcaaaaa tccaattccg ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa
    21721 tgaaagcact gttcaaagaa ccgaatgaca aggcactgaa ctaattacaa gaaccagggg
    21781 cggaaattcc agccctctcg attgttacgt agcacggaca gactatacgc ctggtggtcg
    21841 ttccccatcg ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta
    21901 acgctgctac cctctttttt ctggagtaga tttatgtcta tagactggaa ctggggtatt
    21961 tttttacaac aagccccgtt cggcaacacc acctatcttg gttggatctg gagtggtttt
    22021 caggtgacga tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt cggttcattt
    22081 ttcggcattt tacgtaccgt tccaaaccgt tttctctccg gtctgggtac gttgtatgtc
    22141 gaactgttcc gcaacgtgcc gttgattgtg caattcttta cctggtacct ggtgatcccg
    22201 gagctgctgc cagagaaaat cggcatgtgg tttaaggcag agctggatcc caatattcag
    22261 tttttccttt catccatgct ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag
    22321 gtacgcgccg ctattcagtc gctgccgcgt gggcaaaaaa atgccgcgct ggcgatgggt
    22381 ctgacgctac cgcaggctta ccgctacgta ctgctgccta atgcttatcg cgttatcgtc
    22441 ccgccgatga cctcagagat gatgaacctg gtgaaaaact ccgccatcgc ctcgactatc
    22501 ggtctggtgg atatggcggc gcaggcgggt aaattgctcg attactcggc ccacgcctgg
    22561 gagtcgttta ccgccatcac gctggcgtat gtattgatta acgcttttat catgctggtg
    22621 atgacgctgg ttgaacgtaa agttcgtctg cccggcaaca tggggggcaa ataatgtacg
    22681 agtttgactg gagttccatt gttccttccc tgccatatct gctcgacggg ctggtgatca
    22741 cgctgaaaat caccgtcacg gcagtggtga ttggtatttt gtggggcacc atgctggcgg
    22801 tgatgcgctt gtccagcttt gcgcctgttg cgtggtttgc caaagcctat gttaacgtat
    22861 tccgctcgat tcctttagtc atggttttgc tgtggtttta cctgattgtg ccgggatttc
    22921 tgcaaaacgt gctgggatta tcgccaaaaa atgatatccg tttaatttcg gcaatggtgg
    22981 cgttttcgat gtttgaagcg gcctactatt cagagattat ccgtgccggt attcaaagta
    23041 tttcccgtgg tcagtcgagc gccgcgctgg cgttggggat gactcactgg cagtcgatga
    23101 aactgattat tctgccgcag gctttccgcg cgatggtgcc gctgctgctc actcagggca
    23161 tcgtactgtt ccaggatacc tcgctggtgt atgtgttatc tctggccgat ttcttccgta
    23221 ccgcttcaac cattggtgag cgtgatggta cgcaggttga gatgatcctg tttgccggat
    23281 ttgtttattt cgttattagc cttagcgcgt cgttgttggt cagctacttg aaaagaagga
    23341 cagcataatg attaccctga aaaatgtttc aaaatggtat ggtcactttc aggtgctgac
    23401 cgactgctca accgaagtga aaaaaggcga agtggtggtg gtttgcggcc cgtctggttc
    23461 cggcaaatca acgctgatta aaaccgtcaa cggcctcgaa ccggtgcagc aaggtgaaat
    23521 caccgtcgat ggcatcgtgg ttaacgacaa gaaaaccgat ctggcaaagc tgcgttcccg
    23581 cgtcgggatg gtattccagc atttcgagct gttccctcat ctgtcgatta tcgaaaacct
    23641 gaccctggcg caggtgaaag tgcttaaacg cgataaagcg ccggctcgtg aaaaagcatt
    23701 gaaattgctg gagcgcgtcg ggctttccgc acatgccaat aagtttccgg cgcagctttc
    23761 cggcggtcag cagcagcgtg tggcgatcgc tcgcgcattg tgtatggatc ctattgccat
    23821 gctgttcgac gaaccgacat cggcgctgga tccggagatg atcaacgaag tgctggacgt
    23881 gatggtggaa ctggcgaacg aaggaatgac catgatggtg gtgacccacg aaatgggctt
    23941 tgcccgtaaa gtggcgaatc gggtgatctt tatggacgag ggtaaaattg tcgaagactc
    24001 gccgaaagac gctttcttcg atgatccgaa atcggaccgc gcaaaagact tcctcgcgaa
    24061 aatcctgcat taatcactct ggcgcgcata cccaagtgcg cgccatctca cgctttatcg
    24121 tttcgctcct cgtatctgtt acatcctagc ggtacccttg tcgcaaagaa gcacacaaca
    24181 aggagcaaca atggcactgc caattctgtt agattgcgac ccaggtcatg acgacgctat
    24241 cgcaatagtt cttgccctcg cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc
    24301 cggaaaccag acaccagaaa aaaccttacg caatgttctg cgtatgctga ccttgcttaa
    24361 tcgcaccgat attccggtag caggcggcgc ggtaaaaccg ttaatgcgta atttgattat
    24421 tgcggacaat gtgcacggcg aaagcggtct cgacggcccg gcattaccgg aaccgacgtt
    24481 cgcaccgcaa aactgtacgg cggtggagct aatggcgaaa acgctgtgtg aaagtgagga
    24541 acctatcacc attgtttcaa ccggaccgca aactaacgtt gccttgctgc tcaatagcca
    24601 cccggaactg catagcaaaa ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg
    24661 taactggacg cctgcggctg aatttaatat ttacgttgac ccggaagcgg cagaaattgt
    24721 cttccagtca ggaattccgg tggtgatggc cggtctggat gttactcaca aagcacaaat
    24781 ccacgttgaa gacactgagc gtttccgcgc gattggcaac cctgtttcaa ccattgttgc
    24841 cgaactgctg gatttcttcc tcgaatatca taaagacgaa aaatggggct ttgtcggcgc
    24901 accactgcat gacccatgca ccatcgcctg gctgttgaaa ccggagttgt ttaccactgt
    24961 tgagcgctgg gttggcgtgg aaacacaagg gaaatatacc cagggtatga cggttgttga
    25021 ttattattat ctgactggca ataaaccgaa tgccaccgta atggtcgatg ttgatcgtca
    25081 gggctttgtt gatttactgg ctgatcgtct gaaattttac gcttaatcac tcgccggatg
    25141 acatcacgcc atccggcacc actcatttca ttacggctcg aacggacgtc tctggaactg
    25201 atcatgacca catttcgggc atagcgtcag cacttccggt gtatagatcg ggagatggaa
    25261 gtgacatttc tcgcagacca gatttcccag ccctaccact tctccgctgt gataaacccc
    25321 gtgatgattg aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatgtc
    25381 tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt cagattcttc
    25441 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt
    25501 cagctcatcg acctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
    25561 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca gtgacgcgac
    25621 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
    25681 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
    25741 taatgtatga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
    25801 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
    25861 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
    25921 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
    25981 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
    26041 cacgtacgta actacaccat cggtgacgtg atcgcccgct accagcgtat gctgggcaaa
    26101 aacgtcctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
    26161 aaaaacaaca ccgcaccggc accgtggacg tacgacaaca tcgcgtacat gaaaaaccag
    26221 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
    26281 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataag
    26341 aagacttctg cagtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
    26401 atcgacggct gctgctggcg ctgcgatacc aaagttgagc gtaaagagat cccgcaatgg
    26461 tttatcaaaa tcaccgctta cgctgacgag ctgctcaacg atctggataa actggatcac
    26521 tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggaa
    26581 atcaccttca acgttaacga ctatgacaac acgctgaccg tttacactac ccgcccggac
    26641 acctttatgg gttgtaccta cctggcggtg gctgcgggtc atccgctggc acagaaagcg
    26701 gcggaaaata atcctgaact ggccgccttt attgacgaat gccgtaacac caaagttgcc
    26761 gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agcggttcac
    26821 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggaatacggc
    26881 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
    26941 tacggcctga acatcaaacc ggttatcctg gcagctgacg gctctgagcc agatctttct
    27001 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgagttcaa cggtcttgac
    27061 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
    27121 aaagtgaact accgcctgcg cgactggggt gtttctcgtc agcgttactg gggcgcgccg
    27181 attccgatgg tgacgctgga agacggtacc gtaatgccga ccccggacga ccagctgccg
    27241 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
    27301 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
    27361 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccggagta caaagaaggt
    27421 atgctggatt ccaaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
    27481 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
    27541 ggcatggtga actctgacga accagcaaaa cagttgctgt gtcagggtat ggtgctggca
    27601 gatgccttct actatgttgg cgaaaatggc gaacgtaact gggtttcccc ggttgatgct
    27661 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
    27721 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
    27781 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
    27841 gctgatatga ctctcgaatg gcaggaatcc ggcgtggaag gggctaaccg cttcctgaaa
    27901 cgtgtctgga aactggttta cgagcacaca gcgaaaggtg atgttgcggc actgaacgtt
    27961 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac gatcgctaaa
    28021 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
    28081 ctgatgaaca aactggcgaa agcaccgacc gatggcgagc aggaccgcgc cctgatgcag
    28141 gaagcactgc tggccgttgt ccgtatgctt aacccgttca ccccgcacat ctgcttcacg
    28201 ctgtggcagg aactgaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
    28261 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
    28321 gcaaaaatca ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
    28381 gaacatctgg tagcaaaata tcttgatggc gttaccgtgc gtaaagtgat ttacgtaccc
    28441 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
    28501 cattgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgata
    28561 ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc
    28621 cattaagccg tgcggtgcgt aaccagttac gtctaaatgg tgtcgagttg cttgataaag
    28681 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
    28741 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtcaatg
    28801 cgaccgtgtt gatccccggc cgtgacatct acccgattag cgccaaagtc ttccgttcgt
    28861 tcttcgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
    28921 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
    28981 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
    29041 gcgtctctac cacgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
    29101 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
    29161 ggaaagccag gacgctattc gtcaggtagc tgcggtacaa ggattcgaag aacaccacac
    29221 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggccatgag
    29281 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
    29341 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tgttgatcgt
    29401 ccgcggcaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa
    29461 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
    29521 tgtgcgcgca aaacagctca acttagagct ggatgacgcg gcgaatcagg tgctctgcta
    29581 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctactctg
    29641 gccagacggc aaactgacat tacctcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
    29701 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cgttgcatat
    29761 tcttcagcaa ctgcgtctgg aaggcagcga accggttatt ttgttgcgca cattacaacg
    29821 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catatgccac tgcgtgcgtt
    29881 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
    29941 cttaagccat ccgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
    30001 caaacaagat tacggtcagt cagtgtgggc agagcttgaa gggttatctc ttctgttgtg
    30061 ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt
    30121 ttggcggcac ctttgatccg gtgcactatg gtcatcttaa acccgtggaa acgctggcga
    30181 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
    30241 agccggaagc aaacagcatg cagcgtaaac acatgcttga actggcgatt gccgacaagc
    30301 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa
    30361 cactgaaaga gtggcggcag gagcaaggac cggacgtgcc gctggcgttt attattggtc
    30421 aggattcatt gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
    30481 atttgatcgt ctgtcggcgt cctggttacc cacttgaaat ggcacaacca caatatcagc
    30541 aatggctgga ggatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
    30601 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
    30661 tgcaaaatgg tgaatcgtgt gaggatttat taccggaacc ggtactgact tacattaacc
    30721 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
    30781 tcgtcatggt gaaacgcaag cgaatgtcga tggtctgtac agcggtcatg cgcccacacc
    30841 cctgaccgcg cgcggtattg agcaagcgca aaatctgcat acgctgctac atgatgtttc
    30901 ctttgacctg gttttatgca gtgaactgga acgggcacag cataccgcgc gactggttct
    30961 tagtgaccgc cagcaccccg ttcatatcat ccctgaactc aatgaaatgt tttttggcga
    31021 ctgggagatg cgacatcatc gtgacctcat gcaagaagat gccgaaaact atagcgcgtg
    31081 gtgcaatgac tggcagcatg ctatccccac gaacggtgaa ggatttcagg cattttcgca
    31141 acgtgtggaa cgatttatcg caaggcttag tgaatatcag cactatcaga atattttagt
    31201 cgtcagccat cagggcgtac tgagtctgtt aattgctcgt ttaattggca tgcctgccga
    31261 gtctatgtgg catttccgcg ttgaccaggg gtgttggagc gccattgata tcaaccaaaa
    31321 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaatg catgactttt
    31381 ctgttttttt acgggtaagc cgcaacgacc attgacagcc ccgggcaggc tgatattctc
    31441 cgcagccaga ctttttccgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
    31501 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
    31561 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
    31621 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
    31681 acctcaaagg tcaggacatc atcgccttag acgttcaggg caaatccagc atcaccgact
    31741 gcatgatcat ctgtacgggt acgtccagcc gccatgttat gtccattgct gatcacgttg
    31801 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
    31861 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
    31921 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
    31981 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
    32041 aagatatgcc cttcgagctg attgaaattc cggccggaaa acgcggcaag aatgcggaca
    32101 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
    32161 ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
    32221 aacgctggaa gctggatggt cgcgacgtca gtctactgat tggcgggcct gaagggttgt
    32281 cgcctgcctg taaagcggcg gcggagcaga gctggtcgct gtcggcactt acccttcccc
    32341 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
    32401 accatcctta tcaccgtgag tgataagtga gctttgagta gaaaacgcag cggatgaaac
    32461 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
    32521 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
    32581 aaattgttcg ctttaccgac taccagaccc gctctaatga aaaccgcatt aagctggtgc
    32641 ctatcgcacc cagccgcggc attatctacg accgtaacgg tatccctctg gctctcaacc
    32701 gcactatcta ccagatagaa atgatgccgg agaaagtcga taacgtgcag caaacgctgg
    32761 acgctttgcg cagcgtggtt gatctgaccg atgatgatat tgcggcattc cgaaaagagc
    32821 gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctgacc gaagtacaag
    32881 tagctcgctt tgccgtcaac cagtaccgtt ttccgggtgt tgaagttaaa ggctataaac
    32941 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg tcgaaaatca
    33001 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
    33061 atgatatcgg taagctgggc attgaacgtt actatgaaga cgtgctgcac ggtcagaccg
    33121 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac
    33181 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
    33241 aaacgctgct ggcgggtagc cgcgcagctg tggtagtcac cgatccgcgt acaggtgggg
    33301 tgttggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
    33361 gcaaagatta ttccgccttg ctgaacgacc cgaatacacc gctggtgaac cgcgccacac
    33421 agggggttta tcctcccgcg tctacagtta aaccctatgt tgcggtttcg gcattgagtg
    33481 ccggggtgat cacgcgcaat acgacgctgt ttgaccccgg ctggtggcaa ctgccagggt
    33541 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
    33601 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
    33661 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
    33721 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
    33781 ggtatcaggg tgacaccatt ccggttggta tcggtcaggg ttactggaca gcgaccccaa
    33841 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttccgcatt
    33901 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacaaccg catgaaccgc
    33961 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
    34021 ctaaccgccc taacggtacg gcgcataaat attttgccag cgcaccgtac aaaattgcgg
    34081 cgaaatccgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
    34141 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    34201 cgcaagtggc tgtcgccatg attctggaga acggtggcgc gggtccggcg gttggtacgc
    34261 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat ctgcctgcgg
    34321 aaaatccagc ggttgccgca gcggaggacc attaatcatg acagataatc cgaataaaaa
    34381 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgatcttac tggcattgct
    34441 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    34501 taaaatcggc cagattgcga tgggtctggt catcatggtg gtgatggcgc aaattcctcc
    34561 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    34621 ggtagatgct ttcggtgcca tctctaaagg cgctcaacgc tggctggacc tcggtattgt
    34681 tcgttttcaa ccgtcggaaa ttgccaagat agccgtaccg ttgatggttg cacgctttat
    34741 caaccgcgac gtttgcccac cttcattgaa gaatacagct atcgcgctgg tgctgatatt
    34801 tatgcccacg ctgctggtgg ctgcacagcc tgacttggga acatcaatcc tcgttgcgct
    34861 ttccggcctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    34921 agtgctggta gcggcgttca ttccgattct gtggttcttt ctgatgcatg attaccagcg
    34981 ccagcgcgta atgatgctcc tggacccgga atcagaccca ctcggcgcag gctatcacat
    35041 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcacgg
    35101 cactcagtca cagcttgaat ttctccccga acgccatacc gactttatct tcgcggtact
    35161 ggcggaagag ctgggattag tgggcatttt gattctgctc gctctctaca ttctgctgat
    35221 catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    35281 cggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    35341 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcgg cgctaattgt
    35401 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    35461 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    35521 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    35581 cggtatgtaa cggccccata gttgaaatta gcggggcaga cccgcgtttc gaaccactga
    35641 acgcgacggc aaatcaggat taccagcgcg acgggaaaag ctacaaaatc gtgcaggatc
    35701 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaaccc ggcagtaacc
    35761 tgacggcctc tggcgaagct ttcgatccga cacagttgac ggcggcccat ccaacgcttc
    35821 caatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc gtggtgcgca
    35881 ttaatgatcg cggcctttat ggcaacgacc gcgtgatttc gctttctcgc gcggcagctg
    35941 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    36001 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttacgccc
    36061 tgccagcacc tcccgattta agcggtggcg cgggaacaag ttcagtgcct gtcccgcagg
    36121 gtgacattct tccggtcagt aattcgacgc taaaaagcga agatccgacc ggcgcgccgg
    36181 taaccagcag cggtttcctc ggcgcaccaa cgaccttagc gcctggtgta ctggaaggca
    36241 gcgaaccgac gcctgcgcca cagcccgttg ttacagctcc gtcgacaacg cctgcaacct
    36301 cacctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    36361 aagtcggggc cgttagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    36421 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcggatc cagcttggcc
    36481 cattcgccag caaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    36541 tacagtcatt tattaccacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    36601 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    36661 tagtagggca ctttttttaa ttccatcacg gatgtcgtag ttcagaccat gaataccatt
    36721 ttttccgctc gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac agcctttatc
    36781 tctgctgcac atgccgatga cctgaatatc aaaaccatga tcccgggtgt accgcagatc
    36841 gacgcggagt cctacatcct gattgactat aactccggca aagtactcgc cgaacagaac
    36901 gctgatgtcc gccgcgatcc tgccagcctg accaaaatga tgaccagtta cgttatcggc
    36961 caggcaatga aagccggtaa atttaaagaa actgatttag tcactatcgg caacgacgca
    37021 tgggccaccg gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa accgggcatg
    37081 caggttccgg tttctcagct gatccgcggt attaacctgc aatcgggtaa cgatgcttgt
    37141 gtcgccatgg ccgattttgc cgctggtagc caggacgctt ttgttggctt gatgaacagc
    37201 tacgttaacg ccctgggcct gaaaaatacc cacttccaga cggtacacgg tctggatgct
    37261 gatggtcagt acagctccgc gcgagatatg gcgctgatcg gccaggcgtt gatccgtgac
    37321 gtaccgaatg aatactcgat ctataaagaa aaagaattta cgtttaacgg tattcgccag
    37381 ctgaaccgta acggcctgtt atgggataac agcctgaatg tcgacggcat caaaaccgga
    37441 cacactgaca aagcaggtta caaccttgtt gcttctgcga ctgaaggcca gatgcgcttg
    37501 atctctgcgg tgatgggcgg acgtactttt aaaggccgtg aagccgaaag taaaaaattg
    37561 ctaacctggg gcttccgttt cttcgaaacc gttaatcctc tgaaagtagg taaagagttc
    37621 gcctctgaac cggtttggtt tggtgattct gatcgcgctt cgttaggggt tgataaagac
    37681 gtgtacctga ccattccgcg tggtcgcatg aaagatctga aagccagcta tgtgctgaac
    37741 agcagtgaat tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat caacttccag
    37801 cttgatggca aaacgatcga acaacgcccg ctggttgtgc tgcaagaaat cccggaa
//
LOCUS       DAZKJR010000031        36892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKJR010000031 DAZKJR010000000
VERSION     DAZKJR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.031"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..177
                     /gene="yecU"
                     /locus_tag="ACHOWS_001620"
     CDS             1..177
                     /gene="yecU"
                     /locus_tag="ACHOWS_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFQ0047204.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            164..388
                     /gene="yecF"
                     /locus_tag="ACHOWS_001621"
     CDS             164..388
                     /gene="yecF"
                     /locus_tag="ACHOWS_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFQ0047205.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(455..1177)
                     /gene="sdiA"
                     /locus_tag="ACHOWS_001622"
     CDS             complement(455..1177)
                     /gene="sdiA"
                     /locus_tag="ACHOWS_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFQ0047206.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMGAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCTAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1407..2159)
                     /gene="tcyN"
                     /locus_tag="ACHOWS_001623"
     CDS             complement(1407..2159)
                     /gene="tcyN"
                     /locus_tag="ACHOWS_001623"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFQ0047207.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(2156..2824)
                     /gene="tcyL"
                     /locus_tag="ACHOWS_001624"
     CDS             complement(2156..2824)
                     /gene="tcyL"
                     /locus_tag="ACHOWS_001624"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFQ0047208.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2839..3825)
                     /gene="dcyD"
                     /locus_tag="ACHOWS_001625"
     CDS             complement(2839..3825)
                     /gene="dcyD"
                     /locus_tag="ACHOWS_001625"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFQ0047209.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKIGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3930..4730)
                     /gene="tcyJ"
                     /locus_tag="ACHOWS_001626"
     CDS             complement(3930..4730)
                     /gene="tcyJ"
                     /locus_tag="ACHOWS_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0047210.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4818..5369)
                     /gene="fliZ"
                     /locus_tag="ACHOWS_001627"
     CDS             complement(4818..5369)
                     /gene="fliZ"
                     /locus_tag="ACHOWS_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFQ0047211.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5415..6134)
                     /gene="fliA"
                     /locus_tag="ACHOWS_001628"
     CDS             complement(5415..6134)
                     /gene="fliA"
                     /locus_tag="ACHOWS_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFQ0047212.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6453..8192)
                     /locus_tag="ACHOWS_001629"
     CDS             complement(6453..8192)
                     /locus_tag="ACHOWS_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFQ0047213.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKSDLAAAQLLAPGTADANGTVTYTVSAGLKT
                     STAADVIASLANNAKVNATIANGFGSPTATDYTYNSATGDFTYSATIAAGTNSGDSNS
                     AQLQSFLTPKAGDTANLNVKIGSTSIDVVLASDGKITAKDGSELFIDVDGNLTQNNAG
                     TVKAATLDALTKNWHTTGTPGAVSTVITTEDETTFTLDGGTDATTSGAITVANARMSA
                     ESLQSATKSTGFTVDVGATGTSAGDIKVDSKGIVQQYTGTVFEDAYTKADGSLTTDNT
                     TNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRSS
                     LGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKA
                     NQVPQQVLSLLQG"
     gene            8458..9864
                     /gene="fliD"
                     /locus_tag="ACHOWS_001630"
     CDS             8458..9864
                     /gene="fliD"
                     /locus_tag="ACHOWS_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFQ0047214.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLIIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            9889..10299
                     /gene="fliS"
                     /locus_tag="ACHOWS_001631"
     CDS             9889..10299
                     /gene="fliS"
                     /locus_tag="ACHOWS_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFQ0047215.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            10299..10664
                     /gene="fliT"
                     /locus_tag="ACHOWS_001632"
     CDS             10299..10664
                     /gene="fliT"
                     /locus_tag="ACHOWS_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFQ0047216.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10742..12229
                     /gene="amyA"
                     /locus_tag="ACHOWS_001633"
     CDS             10742..12229
                     /gene="amyA"
                     /locus_tag="ACHOWS_001633"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFQ0047217.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(12263..12676)
                     /gene="yedD"
                     /locus_tag="ACHOWS_001634"
     CDS             complement(12263..12676)
                     /gene="yedD"
                     /locus_tag="ACHOWS_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFQ0047218.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     SALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12863..14068
                     /gene="yedE"
                     /locus_tag="ACHOWS_001635"
     CDS             12863..14068
                     /gene="yedE"
                     /locus_tag="ACHOWS_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFQ0047219.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            14065..14298
                     /gene="yedF"
                     /locus_tag="ACHOWS_001636"
     CDS             14065..14298
                     /gene="yedF"
                     /locus_tag="ACHOWS_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFQ0047220.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            14407..15075
                     /gene="yedK"
                     /locus_tag="ACHOWS_001637"
     CDS             14407..15075
                     /gene="yedK"
                     /locus_tag="ACHOWS_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFQ0047221.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMTAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAVREWMRQEVGGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAAL
                     IQPV"
     gene            15186..15665
                     /gene="yedL"
                     /locus_tag="ACHOWS_001638"
     CDS             15186..15665
                     /gene="yedL"
                     /locus_tag="ACHOWS_001638"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0047222.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PKVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
     gene            15905..16045
                     /locus_tag="ACHOWS_001639"
                     /pseudo
     CDS             15905..16045
                     /locus_tag="ACHOWS_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(16070..16384)
                     /gene="fliE"
                     /locus_tag="ACHOWS_001640"
     CDS             complement(16070..16384)
                     /gene="fliE"
                     /locus_tag="ACHOWS_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFQ0047223.1"
                     /translation="MSAIQGIEGVISQLQATAMSARTQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            16599..18257
                     /gene="fliF"
                     /locus_tag="ACHOWS_001641"
     CDS             16599..18257
                     /gene="fliF"
                     /locus_tag="ACHOWS_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFQ0047224.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            18250..19245
                     /gene="fliG"
                     /locus_tag="ACHOWS_001642"
     CDS             18250..19245
                     /gene="fliG"
                     /locus_tag="ACHOWS_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFQ0047225.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            19238..19924
                     /gene="fliH"
                     /locus_tag="ACHOWS_001643"
     CDS             19238..19924
                     /gene="fliH"
                     /locus_tag="ACHOWS_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFQ0047226.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGVAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            19924..21297
                     /gene="fliI"
                     /locus_tag="ACHOWS_001644"
     CDS             19924..21297
                     /gene="fliI"
                     /locus_tag="ACHOWS_001644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFQ0047227.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGSALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            21316..21759
                     /gene="fliJ"
                     /locus_tag="ACHOWS_001645"
     CDS             21316..21759
                     /gene="fliJ"
                     /locus_tag="ACHOWS_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFQ0047228.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            21756..22883
                     /gene="fliK"
                     /locus_tag="ACHOWS_001646"
     CDS             21756..22883
                     /gene="fliK"
                     /locus_tag="ACHOWS_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFQ0047229.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETPPVINDKQSTSTSLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            22988..23452
                     /gene="fliL"
                     /locus_tag="ACHOWS_001647"
     CDS             22988..23452
                     /gene="fliL"
                     /locus_tag="ACHOWS_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFQ0047230.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            23457..24461
                     /gene="fliM"
                     /locus_tag="ACHOWS_001648"
     CDS             23457..24461
                     /gene="fliM"
                     /locus_tag="ACHOWS_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFQ0047231.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            24458..24871
                     /gene="fliN"
                     /locus_tag="ACHOWS_001649"
     CDS             24458..24871
                     /gene="fliN"
                     /locus_tag="ACHOWS_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFQ0047232.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            24874..25239
                     /gene="fliO"
                     /locus_tag="ACHOWS_001650"
     CDS             24874..25239
                     /gene="fliO"
                     /locus_tag="ACHOWS_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFQ0047233.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            25239..25976
                     /gene="fliP"
                     /locus_tag="ACHOWS_001651"
     CDS             25239..25976
                     /gene="fliP"
                     /locus_tag="ACHOWS_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFQ0047234.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLTGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            25986..26255
                     /gene="fliQ"
                     /locus_tag="ACHOWS_001652"
     CDS             25986..26255
                     /gene="fliQ"
                     /locus_tag="ACHOWS_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFQ0047235.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            26264..27049
                     /gene="fliR"
                     /locus_tag="ACHOWS_001653"
     CDS             26264..27049
                     /gene="fliR"
                     /locus_tag="ACHOWS_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFQ0047236.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            27339..27962
                     /gene="rcsA"
                     /locus_tag="ACHOWS_001654"
     CDS             27339..27962
                     /gene="rcsA"
                     /locus_tag="ACHOWS_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFQ0047237.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(28006..28194)
                     /gene="dsrB"
                     /locus_tag="ACHOWS_001655"
     CDS             complement(28006..28194)
                     /gene="dsrB"
                     /locus_tag="ACHOWS_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFQ0047238.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            28357..28584
                     /gene="yodD"
                     /locus_tag="ACHOWS_001656"
     CDS             28357..28584
                     /gene="yodD"
                     /locus_tag="ACHOWS_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFQ0047239.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            28883..29698
                     /gene="yedP"
                     /locus_tag="ACHOWS_001657"
     CDS             28883..29698
                     /gene="yedP"
                     /locus_tag="ACHOWS_001657"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFQ0047240.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAKQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(29695..31389)
                     /gene="dgcQ"
                     /locus_tag="ACHOWS_001658"
     CDS             complement(29695..31389)
                     /gene="dgcQ"
                     /locus_tag="ACHOWS_001658"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFQ0047241.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(31560..31742)
                     /gene="yodC"
                     /locus_tag="ACHOWS_001659"
     CDS             complement(31560..31742)
                     /gene="yodC"
                     /locus_tag="ACHOWS_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFQ0047242.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(31821..32738)
                     /gene="yedI"
                     /locus_tag="ACHOWS_001660"
     CDS             complement(31821..32738)
                     /gene="yedI"
                     /locus_tag="ACHOWS_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFQ0047243.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            32911..33831
                     /gene="yedA"
                     /locus_tag="ACHOWS_001661"
     CDS             32911..33831
                     /gene="yedA"
                     /locus_tag="ACHOWS_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFQ0047244.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(33820..34290)
                     /gene="vsr"
                     /locus_tag="ACHOWS_001662"
     CDS             complement(33820..34290)
                     /gene="vsr"
                     /locus_tag="ACHOWS_001662"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFQ0047245.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(34271..35689)
                     /gene="dcm"
                     /locus_tag="ACHOWS_001663"
     CDS             complement(34271..35689)
                     /gene="dcm"
                     /locus_tag="ACHOWS_001663"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFQ0047246.1"
                     /translation="MQENISVTDSYSTGNVVQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEVKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(35756..36451)
                     /gene="yedJ"
                     /locus_tag="ACHOWS_001664"
     CDS             complement(35756..36451)
                     /gene="yedJ"
                     /locus_tag="ACHOWS_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFQ0047247.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEEARRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(36491..36772)
                     /gene="drpB"
                     /locus_tag="ACHOWS_001665"
     CDS             complement(36491..36772)
                     /gene="drpB"
                     /locus_tag="ACHOWS_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFQ0047248.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         9012 a         9630 c         9308 g         8942 t
ORIGIN      
        1 atgataaaaa tatttattgg tcattatatt aacgtatttt atagcactgc cgatatcacg
       61 ctcaaaaaac aaccactgct atttttagca aagcttatgg tatactccgc cgccttaaca
      121 tttttcaccg caaattttca ttgcaacatg acgaggaaaa taaatgagta cgcctgattt
      181 ttctactgcc gagaataatc aagaactggc aaatgaagtc tcctgcctga aagcgatgct
      241 gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc cgcgtgatgt taaagatgga
      301 aaaacagctt gcgctgatcg aagacgaaac ccaggctgca gtattttcca aaacggttaa
      361 gcaaattaaa caagcctacc gtcagtaata taaaaccggc tgagagcgtg cctttcagcc
      421 ggtttttgca tctggcacgc agaaagaaaa gcgatcaaat taagccagta gcggccgcgt
      481 aacaggccac ctgggtctta tttggtgcat tgattttttt ctgcatgttt ttctgatgga
      541 aattgaccgt attctcagag attgacaaaa tcatcgctat ctctgctgat gtcttccctt
      601 ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa attcatctct ggcgtcatca
      661 ctatttcatc atttaaacgc atcagagcca tcagactttc gcgcaccagt aactgcattt
      721 ttaattgcag ttcatcacta agaatgggta tttcgcgcgc agtgcaacgg gaaaaggaca
      781 aaaagcccag cgcccggttt ggcagcatta aatactgagt gacaccgcgg cgtaaaccat
      841 gcgcgcgcgc agcttcccat aacggctgtg cttcgctgaa taagtcatca ttccacatta
      901 aatggccctg actaaagttt tcagggttca gtacaggatc aattgcgaga aagttttttg
      961 cctgataata actaacccac gcctcagggt aattggtgta gaaagccact ttaggtcgcg
     1021 tgaatggtac cgggtggcga acacataacg aatagtaatc gtactccagc tgctgagcct
     1081 gaagctctat ttcatggtag acctcttctg cggcccccat cctctgaaaa cgcaacagca
     1141 tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt aaaccgcaac gcccctgaga
     1201 gtgagtatca tttataatga taattcaaac tatcacataa ataagattta tatataattt
     1261 atattattca ggcaatgaat tacttttgca agccatcgca ttctcttatg ttattaatga
     1321 gttatgctga tttgttaagc agttttatca cgtttgaagt ggcgcaccgc cccgacaggt
     1381 gaatcgtcgg ggctgatttt ttcttattat tgcagcagaa acttctcgag gaactggcgg
     1441 gtgcgaggct gctcgggatc ggcaaataac gcttttgcgg ccccctgctc gactatccgc
     1501 ccctggtcca taaagatcgc ccggtccgca acatcccggg caaagctcat ttcgtgcgtc
     1561 acaatcacca tcgtgcgctt ttcctgcgcc agctggcgga tggtgttcag gacttcgccc
     1621 accagctctg gatccagcgc cgacgttggc tcgtcaaaca aaatcacttc aggacgcata
     1681 gccagcgctc gcgcaatcgc gacacgctgc tgttgaccgc cagacaaacg acgtggatag
     1741 ctggtttctt tacctgccag cccaactttc gccagcagct cgcgagcgcg cgccgtggcc
     1801 tcttctttcg gttcgccttt gacgatcacc ggcccttcaa taatgttttc cagcaccgta
     1861 cgatgcggaa acaaattaaa gttctggaag acaaacccga cgtgctgacg caactggcga
     1921 atcagcgatt tttgctgact taatgaacgt gcagtatcaa tggtgatatc gccaacggtg
     1981 atggttcccg cttccggttg ttccagcaga tttatgctgc gtagcaacgt ggttttgccg
     2041 gaaccacttg gaccgataat tgccaccact tcgccaggct tcacctcaag gtcgataccg
     2101 tgcagcaccg tctgaccgtg gaattttttc accaggttct taacttcaat ggcactcatt
     2161 ttggttctct ctcctggcga ttaagttgat tctcaaaatg gttctgcaac gtcgataata
     2221 ctgtcgccat gatccagtag atgagcgaag ccgccaggta catggtgaac acctccagcg
     2281 tgcgcgaggt aatcaactgc gcctgacgga acagctccgg cacctggatt gtcgcggcca
     2341 gcgacgtatc ttttaccagg ctgataaagc tgttcgacag cggcggcaac gccacacgcg
     2401 ccgcctgtgg caaaatcgcg cgacgcatgg tctgccacgg cgtcatacca atactggcag
     2461 cagcttccca ctgaccttta tcaatagaag aaatggcggc gcgcagtgtt tcggcggcat
     2521 aggcggcagt gtttagcgac agaccaatca ttgctgacgg aatgggatct aattcgatgc
     2581 caaactgcgg caggccgtaa tagatcataa acagttgtgc gatgagtggc gtaccacgaa
     2641 aaatagagat ataaaaacgc gccagccagc gcacgggcca gattggcgaa aggcgcatca
     2701 gcgcgagaat aaacccgagc agtaagccaa aaaacatgcc gccgatactg agttgcagcg
     2761 tatacccagc ccctttcagc aggaacggca aggaatcaat aaccagttgt atactttctt
     2821 gcatgagcgt ttccggagct aaacgtgggg atgataggcg aacagcgcag gcgcgccgcc
     2881 ggtatgaata aacagaatcg gcccttcatc tttgaagcgt ttctgactga taccgtcaat
     2941 cagccccgcc attgcttttc cggtatacac aggatcaagc agaatgcctt ccagccgcgc
     3001 cagcaatttc actgcttcca tgccttcgtc gttcggcacg ccgtagccag gtgcaaaata
     3061 gtcatcccag agtaaaattt ccgctgatgc ggtcagctcc agttctttcg caatcgcctg
     3121 ttggaggtta accactttcg gcaattgatc ggcaacggaa cgcgacacgg tcacgccaat
     3181 cagttcgctt tcaggcatca ggtgttccag cccaacagcc agtccggcgt gagttccggc
     3241 actgcccgac gcgaccacca ccgacgaaat attaactgcc ccttcacact gttgcgcaat
     3301 ttccagcgca ctctccacat aacccagcgc gcccaaagca ttagaaccgc caaccggaat
     3361 gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc agctcttcca gttgggcatt
     3421 gggatcggtc agtgcgtcgc acatttcaat ctgggtattg aacagatcca gcaacaaacg
     3481 attgccgttg gttaaatagt tttctgcggt tgtgccaata ggattttcca gcagcgccac
     3541 gcagtgcaga ccgattttcg ccgcaactgc ggcagtctgg cgcacatggt tagactggat
     3601 cgccccggca gtaatcagcg tatcggcacc ttcacgcaga gcatctgccg cgagaaattc
     3661 cagcttacgt aatttattgc cgcccattgc catgggagtg acgtcatccc gtttgatgaa
     3721 aatttcccgt ccaagataat cagaaaagcg cggcagatat tcgagcggcg ttggcgcgcc
     3781 gataaactcc agccgtggaa aacgggttaa attatgcagt ggcataacag cctccgatgt
     3841 gtgttgttgt gattttctta ttatgcacgc tgaaaacgcg taaataaaaa aggcgctagt
     3901 gaaagcgccc ttttttgtca ttatgctgat tatttggtca catcagcacc aaaccatttt
     3961 tcggaaaggg cttgcagagt gccatctttt tgcatttccg caattgcatc attcaccgct
     4021 ttcagcaggt cttcatttcc tttacgcagc gccacaccag actcctgacg ggagaatgct
     4081 tcaccggtta ctgccagcgt atcgttggtt ttcttcacca gatccagcgc cgccagacgg
     4141 tcaacgagga tcgcatcgat acggcctacg cgcagatcct gatatttggt cgggtcatca
     4201 tcataggtac gcacatcgac gccctgaaca ttctgacgca gccactcttc atagttggtg
     4261 cccagaccga cacccacttt tttgcctttc agatcatcag ccgttttaat ggtgccttcg
     4321 ttaccttttt tcaccagcgc ctgaataccg gaaatggtgt acggggtaga gaaatcgtat
     4381 tttttcttgc gctcgtcaga aatggtgacc tggttaatca ccacatcaat gcgtttagag
     4441 tccagcgacg ccagcatacc gtcccattta gtcggtttta gtgacgcctc aacgccaaga
     4501 tgttttgcca gctgttgggc aaattccact tcaaaaccgg ttaatttgcc gtcatccccc
     4561 tgaaaactga acggcggata agttccttcc agccctacca gcagcgtgcc gcgctctttg
     4621 actttattaa gcagaccttc atctgcaaaa cttttaacgc tcatacccgc aaccagcgcc
     4681 acggccatca cacccatcaa tgcctgacgt cccagatgtg ctaatttcat atttaccccg
     4741 aatgttgtta tgtctgtttg cagtgtagag ccattgtttg taaacacaaa aacaactccg
     4801 ctacatctta ttcttattta atatatatca gaagcaggca gactggagga tttctgcaca
     4861 agtccggtac acgtttgacg ctgatagtgt tgatacttcc gtaacgcaat gcggtaattg
     4921 ttggtgctgg tggtgggcag ccacggggcg aggatttcat caaggaaacc gtcctgcagc
     4981 tgatcgaggg aaatattttg ctcgtgtaga tgattaccca aacggcgcag acgaacgaca
     5041 tattcacgca ccgtacctgg gctcatttca gtttgctcaa aaagaaattg cttaaagccg
     5101 ataatatcga aaaaatcact ctgcgttttg caatgtaaat caccgcaaaa tcggcacaat
     5161 gccgcccatg atttttgttc cacttgccag ccattttcat caagcagcat gtccaggcgg
     5221 gaaatctcga ttttattcac tattttgccg tcgcgaacga gggtaatgcg atcgagtaat
     5281 ttacggcaat gcgcgcaatg ggtctggctg tgtttaaagt ctttaagata gcggcttaat
     5341 ggccgtcttt tcaggtgctg caccatcatt aagaactcct ggtagtcaaa attaaagtgc
     5401 ggcatttact gacgttataa cttacccagt ttagtgcgta accgtttaat ggcctggctg
     5461 tgtaactgac tgacccgcga ttccccgacc tccagcaccg cgccaatctc tttgagattc
     5521 agctcttcct ggtaatagag ggttaatacc agtttttcgc gctccggcaa cgtttcgata
     5581 gcttccatca cccgctggcg cagattactg tccagcagtt gttgtagcgg gttttctcgc
     5641 tgatgatcat cagtaaccag ttcgatgcta tcgccgtgct cttcgcgcca ctcatcgtag
     5701 gagaagagct ggctgttatt ggtgtcgagc aacatttggc gatagtcggc aatatcgatc
     5761 cctaaacgtt ccgctacctc agtttccgtg gcgttgcggc caagttcctg ctccagttgc
     5821 cctattgcct gtgccacttc acgcgcgttg cgtcgcacgc tgcgcggcac ccagtcacgg
     5881 ctgcgaagtt catccagcat agcgccacgg atacgctgca ctgcgtaagt tgtaaatgcc
     5941 gttccttgta gggcgtcata gcgttcgacg gcattgagta acccaatgcc gcccgcctgt
     6001 agcagatcgt caagttccac gctcgcgggc agtcgaacct gcaggcgcaa tgcttcgtga
     6061 cgcaccagcg ggacataacg ctgccacagc gagtgtttat ccattacacc ttcagcggta
     6121 tagagtgaat tcacgataaa cagccctgcg ttatatgagt tatcggcatg attatccgtt
     6181 tctgcagggt ttttaatcgg acgattagtg ggtgaaatga ggggttattt gggggttacc
     6241 ggtaaattgc gggcagaaaa aaccccgccg ttggcgggga agcacgttgc tgacaaatta
     6301 ccattcatgt tgccggatgc ggcgtaaacg ccttatccgg cctacaaaat ccgcaaattc
     6361 aataaattgc aattcacctt gtaggcctga taagcgcagc gcatcaggca atttggcgtt
     6421 gccgtcagtc tcagttaatc aggttacgac gattaaccct gcagcagaga cagaacctgc
     6481 tgcggtacct ggttagcttt tgccagcacg gagttaccgg cctgctggat gatctgcgct
     6541 ttcgacatgt tggacacttc ggtcgcatag tcggcgtcct gaatacggga ctgcgcttcg
     6601 gacaggttgg tagtggtgtt gttcaggttg gtgaccgcgg aatccagacg gttttgcacc
     6661 gcaccgaggg aggagcggaa tttgtcgatg gagctgatgg cttcgtccag agctttcagt
     6721 ggatcggcgg tggttgcagc tttagtttca gcgtcagtag taaaattacc atccgctgaa
     6781 acatagactg ctttacctga accattggtc acagttccgt ctttttgcaa aaacagattg
     6841 gttgtattat cggtagtcag tgaaccatca gctttggtgt aagcgtcttc aaaaactgta
     6901 cctgtgtatt gttgtactat acctttacta tcaactttaa tatcgcctgc gctggtacca
     6961 gtagctccaa catcaactgt gaatcctgtg gacttagttg ccgattgaag agactcagca
     7021 ctcattcttg catttgctac agtgattgca ccagaagtag tagcatcagt accgccatcc
     7081 agagtgaagg ttgtttcatc ttcagttgta attaccgtag atacggcacc cggtgtgcct
     7141 gttgtatgcc agtttttagt cagtgcatca agagtggctg ctttgacagt cccagcattg
     7201 ttttgagtga ggttaccatc tacgtcaata aatagttctg aaccatcttt cgcggtaatt
     7261 ttaccgtcgc tagccaatac aacgtcaatt gacgtagaac caattttaac gtttaagtta
     7321 gcagtatcgc ccgcttttgg tgtcaggaag gattgtaact gagcactgtt actatcacca
     7381 gaatttgtac cagctgcaat agttgcacta tatgtaaaat cacctgtagc gctgttgtat
     7441 gtataatctg tagctgttgg cgatccaaaa ccatttgcaa ttgtggcatt aacttttgcg
     7501 ttattagcca aactcgcaat tacatctgca gctgtagatg ttttcaggcc tgcgctaaca
     7561 gtataggtaa ctgtaccatt agcatcagca gtacctggag ccaagagttg agctgctgcc
     7621 aaatcagatt tagtcgctgc agtattagcc acagccccgc caccattcac attaaaccca
     7681 ctcagcccca gcgtatcaga gtcaattttc ttcagatcaa tagtgatagt ctggccgtca
     7741 ttcgcaccaa cctgaatttt cattgaaccg tctttcgcca gtacgttcac gccgttgaac
     7801 tgggtctggc cagatacgcg gtcaatttcg tccagacggg atttgatttc gtcctgaatg
     7861 gagtccagat ccgaatcgga gttagtcccg gtagaagcct gaaccgtcag ttcacggata
     7921 cgctgtaagt tgttgttgat ttcggacagc gcgccttcgg tggtctgtgc aacggagata
     7981 ccgtcgttgg cgttacgtgc agcttgagtc agacctttaa tgttagaagt aaaacggtta
     8041 gcaatcgcct gacctgcggc gtcatccttc gcgctgttaa tacgcaagcc agaagacaga
     8101 cgctcgatag aactcgacag cgcagactgg ttcttgttga tattattttg agtgatcagc
     8161 gagaggctgt tggtattaat gacttgtgcc atgattcgtt atcctatatt gcaagtcgtt
     8221 gattacgtat tgggtttcca cccgtcggct caatcgccgt caaccctgtt atcgtctgtc
     8281 gtaaaacaac ctttagaatt tttttcacaa acagccattt tttgttagtc gccgaaatac
     8341 tcttttctct gccccttatt cccgctatta aaaaaaacaa ttaaacgtaa actttgcgca
     8401 attcaggccg ataaccccgg tattcgtttt acgtgtcgaa agataaaagg aaatcgcatg
     8461 gcaagtattt catcgctggg agtcgggtca ggtctggatt taagttcaat ccttgatagc
     8521 ctcaccgccg cgcaaaaagc gacgctaacc cctatttcaa atcagcaatc gtcgtttacc
     8581 gctaaactta gcgcctacgg tacgctgaaa agcgcgctga cgactttcca gaccgccaat
     8641 actgcattgt ctaaagccga tcttttttcc gccaccagca ctaccagcag caccaccgcg
     8701 ttcagtgcca ccactgcggg taacgccatc gccgggaaat acaccatcag cgtcacccat
     8761 ctggcgcagg cgcaaaccct gaccacgcgc accaccagag acgatacgaa aacggcgatc
     8821 gccaccagcg acagtaaact catcattcaa caaggcggcg acaaagatcc gattaccatt
     8881 gatatcagcg cggctaactc atcgttaagc gggatccgtg atgctatcaa caacgcaaaa
     8941 gcgggcgtaa gcgcaagcat cattaacgtg ggtaacggtg aatatcgtct gtcagtcaca
     9001 tcaaatgaca ccggccttga taatgcgatg acactctcgg tcagcggtga tgatgcgcta
     9061 caaagtttta tgggctatga cgccagtgcc agcagcaacg gcatggaggt ctcggtcgcc
     9121 gcccagaatg cgcagctgac ggtcaacaac gtcgccattg agaacagcag caacaccatc
     9181 agcgacgcgc tggaaaacat caccctgaac ctgaacgatg tcaccacggg caaccagacg
     9241 ctaaccatca ctcaggacac ctccaaagcg caaacggcga ttaaagactg ggtgaatgcc
     9301 tataactcgc taatagatac cttcagcagc ctgaccaaat acaccgccgt agatgcggga
     9361 gctgatagcc agagttctag caatggcgca ctgctcggcg actccacgtt gcggacgatt
     9421 cagacgcagt tgaaatcgat gctgagtaat accgttagtt cttccaacta taaaacgttg
     9481 gcgcagattg gtatcacgac cgatcccagc gatggcaaac tggaactgga tgccgacaaa
     9541 ctcaccgctg cactgaaaaa agatgccagc ggcgtaggtg cattgattgt tggcgatggt
     9601 aaaaaaaccg gcatcacgac caccatcggc agcaacctga ccagttggct ttcgacaaca
     9661 ggcattatta aagccgctac cgatggcgtt agtaagaccc tgaataagtt aaccaaagac
     9721 tacaacgccg ccagcgatcg cattgatgcg caggtcgcgc gctacaaaga gcaatttacc
     9781 caactggacg ttttaatgac ctcgttaaac agcaccagca gctacttaac gcagcagttc
     9841 gaaaacaaca gtaattccaa gtaatcaata ttcatcggga gacaggtcat gtacgcggca
     9901 aaaggcaccc aggcctatgc acaaattggc gtcgaaagcg ccgtaatgag cgccagccag
     9961 caacagctgg tcaccatgct atttgatgga gtgctgagcg cactggttag agcgcgcctg
    10021 tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt tgtcaaaagc gatcaacatc
    10081 attgagaacg ggctgcgggt gagtcttgat gaagagagca aagacgaact aacccaaaac
    10141 ttgattgctc tttatagcta tatggtcagg cgcttgctgc aagccaattt acgcaacgat
    10201 gtctccgcag tcgaagaagt ggaagcatta atgcgcaata ttgccgatgc ctggaaagag
    10261 tctttactct ccccttcttt gattcaggac ccagactgat gaacaatgca ccgcatttgt
    10321 atttcgcctg gcaacaactc gtcgaaaaaa gtcagctcat gttacgcctg gcgacagaag
    10381 aacaatggga cgaactcatc gccagcgaaa tggcgtatgt gaatgcggtg caggagatcg
    10441 cacacctgac tgaagaggtt gccccgtcca ccacgatgca ggagcagctc cgcccgatgc
    10501 tgcgcctgat tctcgacaac gaaagcaagg taaagcagtt attacagatt cggatggatg
    10561 aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt gcctatggcg
    10621 atcagggtgg ctttgtgctg gctccgcagg ataacctctt ttgaatctta aagagccaat
    10681 ggctcgcgaa taatctgatt acggctacgc ttctaatgtt ccccttgaat ggagtcgaag
    10741 aatgcgtaat cccacgctgt tacaatgttt tcactggtat tacccggaag gcggtaagct
    10801 ctggcctgaa ctggccgagc gcgccgacgg ttttaatgat attggtatca atatggtctg
    10861 gttgccgccc gcctataaag gcgcatcggg cgggtactcg gtcggctacg actcctatga
    10921 tttatttgat ttaggcgagt ttgatcagaa aggcagcatc cctactaaat atggcgataa
    10981 agcacaactg ctggccgcca ttgacgccct gaaacgtaat gacattgcgg tgctgctgga
    11041 cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg tgcaacgtgt
    11101 aaatgctgat gaccgtacgc aaattgacga agaaatcatt gagtgcgaag gctggacgcg
    11161 ttacaccttc cccgcgcgtg ccgggcaata ctcgcagttt atctgggatt tcaaatgttt
    11221 tagcggtatc gaccatatcg aaaaccctga tgaagatggc atttttaaaa ttgttaacga
    11281 ctataccgga gaaggctgga acgatcaggt tgatgatgaa ctcggtaatt tcgattatct
    11341 gatgggtgag aatatcgatt ttcgcaatca tgccgtgacg gaagagatta aatactgggc
    11401 gcgctgggtg atggaacaaa cgcaatgcga cggctttcgt cttgatgcgg tcaaacatat
    11461 tccggcatgg ttttataaag agtggatcga acacgtacag gaagttgcgc caaagccgct
    11521 gtttattgtg gctgaatact ggtcgcatga agtcgataaa ctgcaaacgt atattgatca
    11581 ggtggaaggc aaaaccatgc tgtttgatgc gccgctgcag atgaaattcc atgaagcatc
    11641 gcgcatgggt cgcgactacg acatgacgca gatcttcact gggacgctgg tggaagccga
    11701 tccttttcac gccgtaaccc tcgttgccaa ccacgacacc caaccgttgc aagccctcga
    11761 agcaccggtc gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt tgcgggaaaa
    11821 tggcgttcct tcggtgttct atccggacct ctacggtgca cattacgaag atgtcggtgg
    11881 tgacgggcaa acctatccga tagatatgcc aataatcgaa cagcttgatg agttaattct
    11941 cgcccgtcag cgtttcgccc acggtgtaca gacgttattt ttcgaccatc cgaactgcat
    12001 tgcctttagc cgcagtggca ccgacgaata tcccggctgc gtggtggtca tgtcgaacgg
    12061 ggatgatggc gaaaaaacca ttcatctggg agagaattac ggcaataaaa cctggcgtga
    12121 ttttctgggg aatcggcaag agagcgtagt gaccgacgaa aacggcgaag cgaccttctt
    12181 ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa ttcatccccg
    12241 gcggcaagcc ggggagattt cattacggca gtggcgtcgg taacggcgct ttatccagcg
    12301 cggcggcaca ctctgccgtc gggcgatcca cacgctccat cgtcatacca tcgtactcaa
    12361 tggtgttacc gtcacgctca acctcgtaca gctcacgttt cacggtaacg ttagtgagat
    12421 catcagacat caatgtcaac ttacccggca cggcaatcac ccgctgccac tgacggcaat
    12481 ccagggtatc gccctcttta gtgacaatca ggctgcctat cgcttccgga ctgaccagcg
    12541 cactttgcgg ccctttggtc tgccagtagc ccgccagcca gtccggcgcg ggggttttca
    12601 cgacattgtt atagttttcg acttcggcac agcccgccag caacatgagc gcgcctgcaa
    12661 ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg gcattaaagc
    12721 cctgatgtcg ccagcctttc caggttcaga gagaaatttg atctaactat tatttttgtg
    12781 aattattacc ccgaaaataa atttgcttga tgtatgattc gcaactcttg tttcaaacct
    12841 tctgagttca gaggctacat acatgtcatg gcagcaattc aaacacgcct ggttgattaa
    12901 attctgggcc cccatccctg cggtcatcgc ggcgggtatt ctctccactt actactttgg
    12961 catcactggc accttttggg ctgtcacggg tgaatttacc cgctggggcg gtcagcttct
    13021 gcagctgttc ggcgtccatg ctgaagagtg gggttatttt aaaattatcc atctggaagg
    13081 atcgccatta acccgcatcg acggcatgat gatcctcggt atgtttggcg gctgctttgc
    13141 tgcagcgctg tgggccaaca atgtcaaact acgcatgcca cgcagccgta tccgcattat
    13201 gcaggccatt attggcggca ttatcgctgg ttttggcgcg cgtctggcaa tgggctgtaa
    13261 cctggcggcg ttctttaccg gcattcctca gttctcgctg catgcctggt tctttgccat
    13321 cgccactgcc attggttcat ggtttggcgc gcgctttacc ctgctgccca tcttccgtat
    13381 tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac cggatcaggc
    13441 gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct gggcgctgct
    13501 cacggcgatg aaccaaccaa aactggggct ggcaatgctg tttggcgtcg gctttggttt
    13561 actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt ggatcaccgg
    13621 acgtacccat atggcgaaag caatcattat cggtatggcg gtgagtgcca tcgggatctt
    13681 cagttacgta cagttaggcg ttgaacctaa aatcatgtgg gcgggaccaa atgcggtaat
    13741 tggtggttta ctttttggtt ttggcatcgt gctggcaggc ggctgcgaaa ccggctggat
    13801 gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca acgtgatcgg
    13861 ctcaacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca ccgactggga
    13921 caaaatcaac ctgctgaaaa cctttggtcc gatgggtggc ctgctggtga catatttgct
    13981 gttgtttgca gctctaatgt tgattatcgg ctgggaaaaa cgcttcttcc gccgtgcggc
    14041 accgcagatt gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg tcttgatatg
    14101 gtgggtgaac cctgccctta tccggctgtc gcaacccttg aggcgatgcc gcagttaaaa
    14161 aaaggggaga tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa tattccactg
    14221 gatgcgcgta atcacggcta tacggtgctg gatattcagc aagacgggcc gaccattcgt
    14281 tatttaattc agaagtaatc cctcattccc gctgtggccc acagcgggaa catcgttacc
    14341 actctatcac atagatttta cccgccccct ctcctattct tttcagtgtg ctctcaggag
    14401 gtttctatgt gtggacgctt tgcccaatcc caaacgcgtg aagattacct cgcgtttctc
    14461 gcggaagata ttgaacgcga tattccctac gatcccgaac ctattggcag atacaacgtc
    14521 gcgccgggaa ccaaagtcct tctgctcagt gaacgtgatg aacaccttca tctggatccg
    14581 gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa cgcccgcgta
    14641 gaaactgccg caaccagtcg catgtttaaa ccgctctggc aacacggtcg ggcaatctgt
    14701 tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaaacagcc ttattttatc
    14761 tatcgcgctg acggacaacc tgtttttatg accgcgatag gtagcacacc gtttgagcgt
    14821 ggtgacgaag ccgaaggatt tttgatcgtc actgcggcgg cagatcaagg tctggtagat
    14881 attcatgacc gccgcccgct ggttctgtcg ccagaagctg tacgggaatg gatgcggcaa
    14941 gaggttggcg gtaaagaagc ctcagaaatt gctgccagtg gctgtgtgcc agcaaaccag
    15001 ttcacctggc acccggtatc gtgcgcggtg ggtaatgtta aaaaccaggg agcggcgtta
    15061 attcaacctg tttgataagc ctggagatta ttgatcaaca atactgcgtc ataagaaatc
    15121 tctattagac aaagatttca gtacctgttg gcatattgca aaaataacac caatacggaa
    15181 tcgtcatgtt cacgattaaa acagatgatc tcacccatcc agcagtgcaa gcattagtgg
    15241 cttaccatat ttccggcatg ctgcagcagt ctccccccga aagtagtcat gctttagatg
    15301 tgcaaaaatt acgtaacccg aaagtgacat tctggtcagt atgggaaggc gaacaactcg
    15361 caggaattgg tgcgctgaag ttgctggatg ataaacatgg cgaactgaaa tcaatgcgta
    15421 ccgcgccaaa ttatttacgt cggggtgtcg ccagtctgat tttacgccac attttgcagg
    15481 tcgcccatga cagatgcctt catcgcctga gcttagaaac gggtacacag gctggattta
    15541 cggcctgcca tcaactttat ttgaaacatg gattcgttga ttgcgaaccg tttgctgatt
    15601 atcaacttga tccgcacagt cgatttttgt cattgacgct atgcgaaaat aatgaattgc
    15661 catgagccag acgcagcaca ttcttgcatt cgacgtgctg cgcctttatt tattatcagc
    15721 cgcaaatgtc ttgtccttag acgctctttc cacatgcgcc acaagaaacc tctattccag
    15781 tgacacaatt atgcctgatt atttccctag cccggaatat gaataaaaac atcaaataag
    15841 atgaaattat caaaatatta atgttgcgtc tgaggtcgta acaacatccg acgtggtgat
    15901 atgtcaaatg agacactctg atggtcaaag ggtttatcgc gtttacagat cgtggatggc
    15961 agaaaacttt cagaatcagg tttccatttt aaaccagaaa ttaagtgagt ttgtcccatc
    16021 catgccccac gcggtgagat cggatgtaat caacaacagg ttacaaaacc tacacctgca
    16081 tgctcatcac ttcctgatac gccgccacca gcttattacg cacctgaatc cccatttgca
    16141 ttgaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg ccgggttcac
    16201 cgagagtgaa tttttctgcc tgcgtgcggg cggctgtttg tgtatcgctt atgcgatcga
    16261 gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg tggcagtgat tcctgtgtac
    16321 gcgcactcat cgccgtagcc tgtaactggc tgataacccc ttcaatcccc tgtatcgctg
    16381 acattttcat ctcccggata atttctggca gcaaagccta ccagtaagtc aataagacaa
    16441 aggcgctaaa tagcaacaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca
    16501 aataatggga gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt
    16561 gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga
    16621 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg
    16681 ccggctccgc agcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
    16741 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga
    16801 cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgaa gttccggcag
    16861 ataaagttca cgaactgcgc ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg
    16921 tcggtttcga actgctcgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga
    16981 attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa
    17041 aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat
    17101 ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa
    17161 ttagcgccat tgtacatctg gtttccagcg cggttgctgg tctgccgccg gggaacgtca
    17221 cgctggtgga tcagggtgga catctgttaa cccagtccaa taccagcggg cgcgatctta
    17281 atgacgctca gttgaaatat gccagcgatg tcgaaggccg gattcagcgg cgtattgaag
    17341 cgatcctgtc gccaattgtt ggtaacggta atattcacgc ccaggtcacg gcgcaactgg
    17401 acttcgccag taaagaacaa acagaagaac agtatcgccc taacggtgat gaatctcagg
    17461 cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccaggcg
    17521 gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc
    17581 ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat agtgggccgc
    17641 gtagcacaca gcgtaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca
    17701 aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct
    17761 tgccagacgg taaaccgttg cctctcagca acgaacagat gaagcaaatt gaagatctga
    17821 cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc gttaactcgc
    17881 cgttcaatag tagtgaggaa agcggcggag aactgccgtt ctggcaacaa caagcgttta
    17941 tcgatcagtt acttgctgcc ggtcgctggt tgctggtact gctggtggcg tggctgctgt
    18001 ggcgtaaagc ggtacgcccg cagctaacac gtcgcgctga ggcgatgaaa gctgtacagc
    18061 aacaggcgca ggcccgcgag gaagtggaag atgcggtgga agtccgcctg agcaaagacg
    18121 aacaactcca acaacggcgc gctaaccaac gtctgggggc agaagtcatg agccagcgta
    18181 tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa
    18241 ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct gatgaccatt
    18301 ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt gcaaactctg
    18361 agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccga tgtgctggcg
    18421 gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg
    18481 cgttcggtat tggtcaaagc tctgggtgaa gaacgtgccg ccagcctgct ggaggatatt
    18541 ctcgaaaccc gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagc
    18601 gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct ggtgcatctg
    18661 aagcgcgccc aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg
    18721 atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga actgaccgaa
    18781 gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga
    18841 acggcagccg aaattatcaa cctgatgaaa acgcagcagg aagaagccgt tattaccgcc
    18901 gtgcgtgaat tcgacggcga gctggcgcag aaaatcatcg atgagatgtt cctgttcgag
    18961 aatctggtgg atgtcgatga tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa
    19021 tcgctgctga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt cctgcgcaat
    19081 atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt
    19141 ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg ccttgccgaa
    19201 accggcgaga tggtgattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa
    19261 aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccaa tggtcgagtc
    19321 ggaagaaacc attattgaag aggccgaacc cagccttgag cagcaactgg cgcaactgca
    19381 aatgcaggcc catgagcaag gttatcaggc gggtgttgcc gaaggtcgcc agcaaggtca
    19441 taagcagggc tatcaggaag gactggccca ggggctggaa caaggtctgg cagaggcgaa
    19501 gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac
    19561 ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc tggaggcggc
    19621 acgtcaggtg atcggtcaga cgccaacggt ggataactcg gcactgatca aacagatcca
    19681 acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg tgcacccgga
    19741 tgatctgcaa cgtgttgatg atatgctcgg cgcaacttta agtttgcatg gctggcgctt
    19801 gcggggcgat cccaccctcc atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct
    19861 cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac caggagtggt
    19921 gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga agccaaaatg
    19981 gcgcagttgc ctgcggtacg tcgctacggg cgattaaccc gcgctaccgg gctggtgctg
    20041 gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgagcg ccagaacggc
    20101 agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg gctgttttta
    20161 atgccgctgg aggaagtcga aggtgtcctg cccggcgcgc gtgtttatgc caaaaacatt
    20221 tcggctgaag ggctgcaaag cggcaagcag ttgccgctcg gttcggcgtt attaggtcgc
    20281 gttctggacg gcagcggtaa accgcttgat ggcctcccct cccccgatac gacggaaacc
    20341 ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga acatgtgctg
    20401 gacaccggcg tgcgcccaat caatgccctg cttaccgtcg ggcgcgggca gcgtatgggg
    20461 ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc acgttacacc
    20521 cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgag ggcgcgaagt aaaagatttt
    20581 attgagaata tcctcggtgc cgaaggacgt gcacgctccg tggtgattgc cgctccggcg
    20641 gatgtttctc cgctcctgcg aatgcagggt gccgcctatg ccacgcgcat tgccgaagat
    20701 tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg ctacgcgatg
    20761 gcccagcgtg aaattgcgct ggcgattggc gaaccacctg ccactaaagg ttatccaccg
    20821 tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat tagcggcggc
    20881 ggctcgatta ccgcgtttta taccgtgctc accgaaggcg atgaccagca ggaccccatt
    20941 gccgactccg cgcgggcaat cctcgacggt cacattgtgc tgtctcgccg actggcggaa
    21001 gccgggcact atccggctat cgatattgaa gcgtcgatca gtcgcgcaat gacggcgttg
    21061 atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc gagttttcag
    21121 cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgac
    21181 aaagccatcg ccctgtggcc gcagctggag ggctatttgc aacaaggcat ttttgaacgc
    21241 gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt atcataaccc
    21301 aggagataac ggcagatgac agaacatggc gcgctggcga ccctgaaaga tctggcagaa
    21361 aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcggggatg tcagcaggcg
    21421 gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa cctcaatagc
    21481 gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg
    21541 ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca gaaagtagac
    21601 attgccctga acagctggcg agaaaagaaa caacgtttgc aggcttggca gacactgcag
    21661 gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat
    21721 gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcatagcg cccttgatta
    21781 ccgctgacgt tgataacgcc acattgcctg gcggcaaagc cagcgatgct gcacaagatt
    21841 ttctcgcgtt gttgagcgaa gcattagcag gcgagacaac aaccgacaaa gcggcccccc
    21901 agttgctggt ggcaacagat aagcccacga caaaaggcga gccgctggtc agcgatattg
    21961 tttccgatgc gcaacaagca gatttactga tccctgtgga tgaaacaccg cctgtcatca
    22021 acgacaaaca atccacatca acatcgttaa ccaccgctca gacgataacg ttggctgcgg
    22081 cggctgacaa caatacggca aaagacgaaa aagcggatga tctgaatgaa gacgtcaccg
    22141 caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg
    22201 atgcaccgtc aaccgtgtta ccgacagaga aaccaacgct tttcacaaaa ctgacttctg
    22261 cgcaactcac tacagcacag cctgatgacg cccccggcac accagctcag ccattaacac
    22321 cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacgccttcg tcggtgaccg
    22381 ctgccgccag cccgctaatc actccacacc agacacagcc actgcccacc gtcgccgcgc
    22441 ctgttttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc cagcatattt
    22501 cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcaccca caggatttag
    22561 gtgaagtgca aatttccctc aaagtggatg ataaccaggc gcaaatccag atggtttcac
    22621 cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt acgcagctgg
    22681 ccgaaagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt agtggtcagc
    22741 agcaggccgc ttcccaacaa cagcaaagcc aacgcacagc aaaccatgaa cctctggcgg
    22801 gggaagacga cgatacgctt ccggttcccg tctctttaca agggcgtgta acaggcaaca
    22861 gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg
    22921 ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc
    22981 gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat
    23041 tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca
    23101 tcaggttgcc gctgaagaca aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc
    23161 gctggatacc tttacggtca atctgggcga tgcggatcgc gtactttata tcggcataac
    23221 cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc cggaagtccg
    23281 tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga cagaagaagg
    23341 taagaaaaac ctgattgccg agattaaaac cacactttcc accccgcttg ttgccgggca
    23401 accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat aacgacatgg
    23461 gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca
    23521 aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata
    23581 cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg
    23641 cccgccattt tcgtatgggg ctgttcaacc tgctgcgccg tagcccggac attaccgtcg
    23701 gggccatccg cattcagccg taccatgaat ttgcccgcaa cttgccggtg ccgaccaacc
    23761 tgaaccttat ccaccttaaa ccgctgcgcg gcacagggct ggtggtgttc tcaccgagtc
    23821 tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg accaaagtgg
    23881 aaggtcgcga gtttacccat acagaacagc gcgtcatcaa ccgcatgtta aagctggcgc
    23941 ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag tacgtacgtt
    24001 cggaaatgca ggtgaaattt accaatatca ccacctcacc gaacgacatc gtggttaaca
    24061 cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt ctgccattca
    24121 gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg
    24181 aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggagctgg
    24241 tcgccaactt tgccgatata tcgctacgcc tgtcgcagat tttaaaactg aaacccggtg
    24301 acgtcctgcc gatagaaaaa cccgaccgca tcatcgccca tgttgacggt gtcccggtgc
    24361 tgaccagtca gtacggcacc ctcaacggtc agtatgcgtt acggatagaa catttgatta
    24421 acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc
    24481 cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg aacaaaaatc
    24541 aaccagcggc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag
    24601 cggaacgttg caggatatcg acctgattat ggatattccg gtcaagctga ccgtcgagct
    24661 gggccgcaca cgaatgacca tcaaagagtt attgcgtctg acgcaagggt ccgtcgtggc
    24721 gctggacggc ctggcgggcg aaccgctgga tattctgatc aacggttatt taattgctca
    24781 gggcgaagtt gtcgtagtcg ctgataaata tggcgtgcgg atcaccgata tcattactcc
    24841 gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accatgctac tgtgcaatct
    24901 tccgcgccgg tttctactgc gccactgctg caggtgagcg gcgcactcat cgccattatt
    24961 gccctgatcc tcgctgctgc ctggctggta aaacggctgg gatttgcccc gaaacgcact
    25021 ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg
    25081 gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg
    25141 ctgcataaac ttcccccttc agcaccaacg gaagagatac cgcagaccga ttttcagtcg
    25201 gtcatgaaaa acttgcttaa gcgtagcggg agatcctgat gcgtcgtttg ttgtctgtcg
    25261 cacctgtcct tctctggctg gttacacccc tcgccttcgc gcaactgacg ggtattacca
    25321 gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca
    25381 ttacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc acccgcatca
    25441 tcattgtttt tggtttattg cgtaacgcac tgggaacacc ctccgcgcca cctaaccagg
    25501 tattgctggg gctggcactg tttttgacct tttttattat gtcaccggtg atcgataaaa
    25561 tttatgtcga tgcgtaccag ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg
    25621 aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgtgag gcggatttag
    25681 gattgtttgc cagactggca aatactggcc cgttgcaggg acctgaagcc gtgccgatgc
    25741 gcattttgct cccggcttac gtgaccagcg agttgaaaac cgcatttcag ataggcttca
    25801 cgatcttcat tccgtttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg
    25861 ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg ctgtttgtac
    25921 tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag
    25981 gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa gtcgcgctgg
    26041 cactggctgc cccgctattg ctggtagcat tggtcacggg ccttatcatc agtattttgc
    26101 aggccgccac gcagattaac gaaatgacgc tgtcgtttat tccgaaaatc atcgccgtat
    26161 ttatcgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca
    26221 ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc aggtgacaag
    26281 cgatcaatgg ctttcctggc taagtctgta cttctggccg ttactgcgcg tgttggcgct
    26341 gatctccacc gcgccgattc tgagcgaacg cagcgtaccg aaacgggtaa aactgggtct
    26401 ggcaatgatg atcacgttcg ctattgcccc atcattacct gccaacgatg ttcctgtttt
    26461 ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg cgcttggttt
    26521 taccatgcaa tttgcctttg ccgcggtgcg aaccgctggt gaaattatcg gtctgcaaat
    26581 ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat cttaatatgc ccgttttagc
    26641 gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc atttatggtt
    26701 gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac cgttgaacag
    26761 caatgcgttt ctggcactca ccaaagcagg gagtttgatt ttccttaacg ggctgatgct
    26821 ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt tactaaatcg
    26881 tatggccccg caattatcca tttttgttat tggatttcca ttaactctga ctgtcggtat
    26941 ctctttaatg gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga
    27001 gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat tccgtaacgt
    27061 ttatcatgct accctaagga ttatccgaaa aataatacct acgaacatct tccaggatac
    27121 tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta ctttacttta
    27181 agacaattcc aggcaaatta tacaacactt tacgggatag taagtccgcc tgaaaaatcg
    27241 cgagagtggc gcattaggtg acccatgttg ttccgtttag tcatgatgaa atattcaggt
    27301 aaggggaatt atcgttacgc attgagtgag ggtatgccat gtcaacgatt attatggatt
    27361 tatgtagtta cacccgacta ggtttaaccg ggtatctgtt gagtagaggg gttaaaaaaa
    27421 gagaaatcaa cgacattgaa accgttgatg accttgccat agcttgtgat tcacagcgcc
    27481 cttcagtggt gtttattaat gaggactgtt tcatccacga tgcttctaac agtcagcgca
    27541 tcaagcacat cattaatcaa catcccaata cgttatttat cgtttttatg gcaattgcca
    27601 atgttcattt tgatgaatat ctattggtca gaaaaaattt attgatcagt tctaaatcga
    27661 ttaaaccgga atctctcgat gatatccttg gcgatattct gaaaaaagag acaacgataa
    27721 cctcgttttt aaatatgccg acgttatcat tgagccgaac cgaatcgagt atgttgcgaa
    27781 tgtggatggc aggtcaggga accattcaaa tctccgacca aatgaatatc aaagccaaga
    27841 ccgtttcatc gcataaaggt aatattaaac gtaagatcaa aacgcataat aaacaggtta
    27901 tctaccatgt cgtccgactg acggataatg tgactaatgg tatttttgtc aacatgcgct
    27961 aacacattct gactggtggt ttcccaccag tcaggctgaa ttagattact ctgctttctc
    28021 cacaaagata ccgtcctgat gccctgcttc attaaagaac cagatgccga gcgggtagtc
    28081 ttccagcgaa accaggtaca ttgtgccttc actaaactcc tcaactgcca gtaccacgcc
    28141 aggacgacgc ggaccgccat ccgttttgac tgttacccga tcattcacct tcatcgtttt
    28201 cctcctgtgg ctttgtgcca gtgtagaaca atttcgtttt tctggcagcg ccaggcgcgc
    28261 gcgagtgctg attttctcga cggtctatac ttaagagatg ccaggcggac ttaacgactg
    28321 gcggcaacaa cagagtaacg gttgcgagga aagatgatga aaaccgcaaa agagtacagc
    28381 gataccgcaa aacgtgaggt cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa
    28441 attagcgaaa gcgaagttca tcgcagccag aacgattctg aacacgttag tgtcgatgga
    28501 cgtgaatatc atacatggcg tgaattggcg gatgccttcg aactggatat tcatgacttc
    28561 agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa atcccgaccc tgagggggtc
    28621 gggatgaaac ttgcttaagc aagaagcact taaaaaattc gttacaccag gaaatctgat
    28681 gtgttcatca ccttatccgc aatttttttc gctgacaaga aaatattcgt gatatgaatg
    28741 attaacgcta cttatgaata gaaatgtgac gcgcgtcacc ccttggtggc tctggcgtca
    28801 acgggatcgc tcccaaaaaa atcatctttg ataatttgtg ctgttttgag aatcatcctg
    28861 gtaaggggag tactgcgcaa ccatgctttc aattcaacaa ccactactgg tttttagcga
    28921 tcttgatggc accctgctgg acagtcatag ttatgactgg caaccggcag ccccctggct
    28981 cagccgttta cgcgaagcga atgttcccgt cattctctgt agcagtaaaa cttcagcgga
    29041 aatgctgtac ttgcaaaaaa tgttggggct acaaggctta ccgctgattg cagagaatgg
    29101 cgcagtgatc cagcttgcta agcaatggca ggagatagac ggttttccac gcatcatctc
    29161 aggtattagc catggcgaaa tcagccaggt tttaaatacg ctacgcgaga aagaacattt
    29221 taaattcacg acttttgatg atgtcgacga tgcaaccatc gccgaatgga cgggattaag
    29281 ccgtagccag gcggcgctga cgcagctgca tgaggcgtcg gtaacgctaa tctggcgcga
    29341 cagtgacgag cgtatggcac aatttaccgc tcgtctgaac gaactgggct tgcagtttat
    29401 gcagggtgcg cgcttctggc acgtcctgga tgcctctgcc ggaaaagatc aggctgccaa
    29461 ctggattatc gcgacctatc aacaattgtc aggcaaacgc ccaaccacac ttggcctggg
    29521 cgatgggcca aacgatgcgc ccttactgga ggtaatggat tacgcggtga ttgtgaaagg
    29581 gctaaaccgt gaaggggtcc atctgcatga tgaggatccg gcccgcgtct ggcgaacgca
    29641 gcgtgaagga ccggaaggct ggcgtgaagg gctggaccat tttttctccg cccgttaagc
    29701 gttatcgctc gcgaataccc gattgcgccc ggcctgtttt gcgaggtaaa gccgacggtc
    29761 ggccaatgac tggagttgtt caaaatcata atcaccggtt tcctcgctgc tgcttacccc
    29821 cagcgaggca ctgatgcgta tcgtcgtact cttggcgatc agcatctctt tttcatttaa
    29881 cttcaggcga atacgttctg cgacctccgc agcctgcgtc agattcgcgc ctggcagaat
    29941 cacacaaaac tcctcaccac cgacccgccc ggcaacgtcc tgcgcacgta aggaactgct
    30001 aattaatccg gcagcatgag aaagaacacg gtcgcccacc tgatgaccaa agcggtcatt
    30061 aatcgcttta aaatggtcaa gatcgacctg aatgacagaa aatggatgtt ggtgcgtctg
    30121 acacaattta gcgagcggac gggctttttc gaacagtgcg ccacggttat ataaacgcgt
    30181 taaggtgtcg tgccacgcct gccactgcaa cgagctttgc agaacataca tgttgctgac
    30241 catccggcga atcacatacc aggagagtag taacatggtg gtaaagagcg cccacagcag
    30301 ggttaatgca atgctgatac tgccgaaatc accacgcacg ccttcgctta gcgtatggac
    30361 gcgcaccagc acaccgtcga aatggtccag acgttcccag ctaacataac gactgttcat
    30421 acgaatgcca ccccgcgtgt catgttccat tgcctgcgcc aacaaggcca gttcacgagg
    30481 atcaaaaata ttccctgttg gatggtcagg attggaagag gtcaaaaatc tcagcttact
    30541 gtcatagagc tgatactcac catcgaggtt tttatcgatg gcgtttctta aaaattgctg
    30601 catggtacgc acgggaatac tcatccccag cacgccatac cagtagtttt cactgtctac
    30661 cggaacactg acggtaacct gcggttcagt attgctggcg tgttccggtt gcgaagtgaa
    30721 ccagcgtacc gcgcggtgac gattttctcg ttgcgaatgg ccgataaacc agggttgggt
    30781 gacatagccg taataacgcg ttggtacatt gcgcgtaaac aaggtcggct gcgtcgaaac
    30841 gtaaaatccg gcacgcgaga catacatcgc ctgttcaacc atcgacgagg agttgtgcgc
    30901 cagtcgcagc aagtaaccaa cttccagtgc agcggtaatt tcattgtcga ggctttcatt
    30961 ttcgcgagac aggagattcc cctcgctgac taatgcatcc gacacgccat tgactggcag
    31021 ggtgcgtcgt cggttgagtt cgatttgcca ggcgtgctcg tcgcgatgct gttcgaactc
    31081 ggttacggca ttacgcagag aggtgaaatc cagtggcgct acgagagctt cgcgcatgcc
    31141 attacgcaaa aagatcagtt tgtcgacgtt atactgcaaa tgcttatcga gcgcgttggc
    31201 tacgttttcc agatgattac gctggctgga gatataggca tcttccagca ccaccacttc
    31261 acgccaggtg agcaaggtgg aaaaaagcaa taccacgata aagcagagat taacgacatg
    31321 accaggcccc aggcggcgtg cgagtttttt caaccagctc tggttttcca tttttgtctc
    31381 gtgctgcacc ctgacgactc ccaacctgct tttttatgat tctggcataa cccggcgata
    31441 acgtaccgca aaacagcgat aattttataa tggtggcatg ataagacgcg actatcattc
    31501 attgcaggca ttggcacacc aggccggatg tgatccggcc tggtgttaat gatacccttt
    31561 caaacttctt ccgcagaacg actcccctcc cccggaacaa gatcggtttc atgaaaagct
    31621 tcccgcttga ccccgtaacc gtcataccaa cgacactcaa ccataccgct ggagtatcca
    31681 gtgacaatca tccgcgggcc gccctcttta accgtaactt cctcactaac cataaagctc
    31741 atactcgcct ccttttttcg agtgaaactt gttcacctta gttgaagatg gcgaattttg
    31801 cataatagcc agtgcgagta ttaatgtgcc tgaccgcgca ttttcgctac tgctttcact
    31861 cccagcacca cgatgccgcc gatgataaaa ccaagaatca gatttaaaac agtcggtaat
    31921 atcatcgcca ccactgcact ttgctgcccg gcgaaatgtt caatggcgtg atgcagcggc
    31981 gcaataccat gtaccacaat cccgccgccg acgaggaaca tcgccagcgt gccgacaatc
    32041 gataatgctt tcatcagcca gggcgcgata atcaataacc ctttacctaa tgcctgcatc
    32101 agcgcgctgg atttttccgc cagccaatac cccaggtcat caatcttaac gataacccct
    32161 accagaccgt agacgcccac ggtcactacc agcgcgatgc ctgaaagcac cagcacctga
    32221 ttaagcaacg gcgcctctgc cacaatcccc agcgtgatgg caacgatttc cgcagacaaa
    32281 ataaaatcgg tacgaatcgc ccctttaatt ttgtcctttt caaacttcag cggatcctgc
    32341 gccgccagct tctccagacg ctgctgactc tgcgccggat cttctttatg tttacgcgcc
    32401 tccagcatat gcagcacttt ctctacccct tcaaagcaga gaaacgcgcc gccaatcatc
    32461 aacagtggcg taatcgccca cgggataaac gcactgatga tcagcgccag cggcaccaga
    32521 atcactttat tgatcagcga tcctttcgcc acgccccaga ccacgggaag ttcccggttg
    32581 gcccgcacgc ctgaaacttg ttgcgcattg agcgataagt catcccctaa tacaccggcg
    32641 gttttcttcg ccgccagttt gcccatcacg gaaatatcgt ccagcagtgt ggcgatatca
    32701 tcgagcaacg tcagtaaact actcccggcc aaaagcattc cctctcattt ttattgttga
    32761 agggtgtaag tatgaagcaa aaacggcagc tctgatactt gcttcacagg tcaacatttt
    32821 tttaacaatc aagtgcaatt atatctctcg ccagcagaat gattttcacg tttactatga
    32881 cccacctttt tatttcagct gtgagggatt atgcgtttcc gccagttgtt accgcttttt
    32941 ggcgcgctgt ttgcgttgta tatcatttgg ggctcaacct attttgtcat tcggattggc
    33001 gtggaaagct ggccgccgtt aatgatggcg ggcgtgcgat tcctggcagc cggtatttta
    33061 ttgctggcat ttttgctact gcgcggacac aaacttcccc cgctacgtcc gctgctcaat
    33121 gccgcgctga ttggcctgtt attgctggct gtcggtaatg gcatggtgac ggttgccgaa
    33181 catcaaaatg ttccttccgg catcgccgcc gtagtggttg caaccgtgcc cctctttacc
    33241 ctgtgcttca gccgcctgtt tggcattaaa acgcgcaaac tggaatgggt gggtatcgcc
    33301 attgggcttg ccggaatcat catgctcaat agcggtggaa atttaagcgg caatccgtgg
    33361 ggcgcgattc tgattttaat cggctcgatt agctgggcgt ttggctcggt ttatggctcg
    33421 cgcattacct tacctgtagg gatgatggcg ggtgcgattg agatgcttgc ggcaggcgtg
    33481 gtgttaatga ttgcgtcgat gattgcgggt gaaaaactga cggcgctccc ttccctttca
    33541 ggcttccttg cggtcggcta tctggcgctg tttggttcga ttatcgccat taacgcttat
    33601 atgtatttaa tccgcaacgt cagtccggct ctcgccacca gctacgccta cgttaacccg
    33661 gtggtcgcgg tcttactggg tacgggactg ggtggcgaaa cgctgtcgaa gattgaatgg
    33721 ctggcgctcg gcgtaattgt tttcgcggtg gtactggtca cgttgggaaa atatctcttc
    33781 ccggccaagc ccgttgtcgc gccagttatt caggatgcat caagcgagta aatgaatccc
    33841 ctgcgtgtcg atctgcgcgc tggcaccttc gccgcagatc cactcttcca gacgctcggt
    33901 aagcgccgca tccgtaagct tctcgcgccc acgtaacgca cactcccaga caatcagtac
    33961 gcgccagccg agttcctgca agcgactgat atcgcggcga tcgcgttcaa catttttacc
    34021 tatcttctcc agccagaact cggttcgagt cgcaggcact ttaaacagat agcagtgatg
    34081 atgatgccag aagcagccgt gggtaaatat cacgcagcga tattcatcaa cgacaaaatc
    34141 cgggcgtccg ggcagactgg cgtcctgaac gcgaaacgca aggccctgcc cggttaacaa
    34201 actggcgagg cgcttctcaa tcgccgtatc acgcgtggca atcgcgcgca tatttttgct
    34261 gcgggtggcc ttatcgtgaa cgtcgaccat gttgtgcctc ttgctgacgc aacgccaccg
    34321 cctgtttgat ttttggctca agcagttttg ccaccgcggc aaagaccggc acgaccaccg
    34381 agttaccgaa ctggcgatag gcctgagtgt ccgaaaccgg aatgcggaat ttcacttctc
    34441 ctggcgcttc aaaacccatt aagcgcgcac attcccgagg cgttaaccga cgtgggcgat
    34501 gttgctgatt cagcggatcg tcaaagtctt tttcacccgt ggccatatcc cagccgcgat
    34561 cgattaaaat ttccgcgcca tctttgtaat aacgagcaga cagcgtacgc gtgacgcttt
    34621 gcggattgtt cggataaacc attccataac cgaagccatt accgcgcgcc tgatgttttt
    34681 tcgcataacg atagaggtac ttccacagca ccggcgtcag gatatatttc gcctcgacca
    34741 tcgggtccag cagctgggcc agcgtcactc gctgcgcagg gaaacattcg ctgatatcac
    34801 gcagggtaaa atcggctttc agattcagat cgcgacgaaa acccaccagc acgatgcgtt
    34861 cacggtgctg cggcaggaaa tgtttgccat cgatgatttt cggatcgtcc ggcccgttat
    34921 cttctgcatc agccacgtca tagcccagtt cgtccagcgt ctgcatgatg atgcggaacg
    34981 ttttaccctg gtcgtgactt ttcaggtttt tgacgttttc gagcacaaac atcgccggac
    35041 gacgcgcgtc gataatgcgc accacatcaa agaacagcgt gccctgagta tcgcaggcaa
    35101 aaccgtgcgc ccgcccgagc gagttctttt tcgatacgcc agccagcgaa aatggctgac
    35161 aaggaaaacc cgccagtaat acatcgtgtt caggaatgtg ttgacgaata tgttccgccg
    35221 ccgcctcatc actcacgcct tctttatggc tgagggtgat gtcgcggata tcttcattaa
    35281 aatgatgcgt cgccgggtcg caataatgat tggctttata agtgcgtacc gcatgtttgt
    35341 tccattcgct ggtaaacacg cactgtccgc caatcgattc aaaaccgcga cggatgccgc
    35401 caattccggc aaatagatcg ataaagcgaa acgcataatg cggatgatgt gccggtggtt
    35461 tgggtaacaa cgtttgcaga tgggcgaact ctttctcact taaacggtgc catgccgagt
    35521 cattcgccag cgcacgtttt aaaattgccg cgctccagtg attctcaccc acaccattaa
    35581 gctgcgccac cagcgtttta acatcataaa tttgcagcag tttctccagc attgcctgaa
    35641 cgacattccc ggtgctgtat gaatcggtta ctgatatatt ttcctgcata gatttaaccg
    35701 gccatctgag atggccgaca ggttaacata attacagcca tttagggcca cgcgctcagc
    35761 ccgcgggtga aaacgcatct atcaccttgt gatcgacacc ttcattctcc cccgccagtt
    35821 cggcactgag cttcgccata aactcgacta aaaattgcgc gttgtgctgc gccagctgct
    35881 tgcctcttgc ggtttgcatg gtttgcggta gcttcagcaa cttagtctgg aaatgatcca
    35941 gcgcgtagcg tttatcatcc agcgggcgat gctgtgcgaa cggatcttca ccatcaaaca
    36001 gcgccacgcc caatgcccct gaaacagcaa acacgcgcgc caggccaatc gcccccaggg
    36061 cttccagccg atccgcatcc tggacgattt tagcttccgt tgttaagggg gcgatttgcg
    36121 cgctgaaact gtgagcggca atggcatgac aaacggcctc gattttctcc gccggaaatt
    36181 gcacgaactc ttcacggagc aggcgacgcg cctcttctgc cgccaggatt gaagaatgct
    36241 gccgttgcgg atgatttttc gccaggctca caatatcgtg aaaataacag gcggttaaaa
    36301 tcaccagcat atcgacatcg tcatctgccg ccagcttctg tgccgtagcc cagacgcggc
    36361 gaaaatgaca tacgtcatgg gccgcgtcct ggtgctgatg atggtttttc aaccagtttt
    36421 cgaactgtgc ctgccagtgt tgtaagtcca tacgcgctcc gttgccaaag agtcgcaacc
    36481 ttagcagttt ttattcatag cgtctgctac gtgcgggacg aggtcgggtg taccaggcaa
    36541 tgctgcctaa caatgccccg accagagcca taaataaaaa tccgaccagt gcccctaacc
    36601 actttcccgc ggtagagcct aaatcgcttt caaatcccga caccggggca tcagggatcc
    36661 ggctcatcac ccagatgtaa cgcgccattg cccagacgaa atagccacac catgtataga
    36721 aagcccacaa agccaatttg ccgccagggc tgcgagagag tctctcttcc atctttgtcg
    36781 aaccgtattc cattttgaga taacccggaa aattcagact tagtactatt atgtgataaa
    36841 agtcacattt ttccacattg gatgagccag tcaagactca atcatttttt ga
//
LOCUS       DAZKJR010000032        36868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKJR010000032 DAZKJR010000000
VERSION     DAZKJR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.032"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            164..754
                     /locus_tag="ACHOWS_001666"
     CDS             164..754
                     /locus_tag="ACHOWS_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0047249.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFSNSKGTLNNDMSINNPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDATS
                     KTATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            863..1603
                     /gene="yadV"
                     /locus_tag="ACHOWS_001667"
     CDS             863..1603
                     /gene="yadV"
                     /locus_tag="ACHOWS_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFQ0047250.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGIPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDVKMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1638..4238
                     /gene="htrE"
                     /locus_tag="ACHOWS_001668"
     CDS             1638..4238
                     /gene="htrE"
                     /locus_tag="ACHOWS_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFQ0047251.1"
                     /translation="MTIKSTKKYHNHLTRIATFCALLYSNSALCAELVEYDHTFLMGK
                     DASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLL
                     QFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASG
                     NYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVT
                     IEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWN
                     KLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLN
                     EDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTN
                     RISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADI
                     YDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSI
                     ILNGLRCQIQ"
     gene            4255..4827
                     /locus_tag="ACHOWS_001669"
     CDS             4255..4827
                     /locus_tag="ACHOWS_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0047252.1"
                     /translation="MIKTTPHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            4839..5447
                     /gene="yadL"
                     /locus_tag="ACHOWS_001670"
     CDS             4839..5447
                     /gene="yadL"
                     /locus_tag="ACHOWS_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFQ0047253.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            5474..6070
                     /locus_tag="ACHOWS_001671"
     CDS             5474..6070
                     /locus_tag="ACHOWS_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFQ0047254.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            6096..7385
                     /locus_tag="ACHOWS_001672"
     CDS             6096..7385
                     /locus_tag="ACHOWS_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFQ0047255.1"
                     /translation="MQHDNGVKMKIFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINDHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLKNTKSAQMVLKPNDSTSVYKDYETEDDTTGGIYPDKG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            7497..8291
                     /gene="panB"
                     /locus_tag="ACHOWS_001673"
     CDS             7497..8291
                     /gene="panB"
                     /locus_tag="ACHOWS_001673"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFQ0047256.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8303..9154
                     /gene="panC"
                     /locus_tag="ACHOWS_001674"
     CDS             8303..9154
                     /gene="panC"
                     /locus_tag="ACHOWS_001674"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFQ0047257.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(9236..9451)
                     /locus_tag="ACHOWS_001675"
     CDS             complement(9236..9451)
                     /locus_tag="ACHOWS_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0047258.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGFQKGYKEGLQKVRLAQRLL
                     SKGMSREDVAEVTALSLAEIDKLINSN"
     gene            complement(9520..10464)
                     /locus_tag="ACHOWS_001676"
     CDS             complement(9520..10464)
                     /locus_tag="ACHOWS_001676"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFQ0047259.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVRQEVIQEV
                     RQEVRQEYALRFLSKGMSREDVAEMANLPLAEVDKLIN"
     gene            10738..11118
                     /gene="panD"
                     /locus_tag="ACHOWS_001677"
     CDS             10738..11118
                     /gene="panD"
                     /locus_tag="ACHOWS_001677"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFQ0047260.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(11122..12351)
                     /gene="yadE"
                     /locus_tag="ACHOWS_001678"
     CDS             complement(11122..12351)
                     /gene="yadE"
                     /locus_tag="ACHOWS_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0047261.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12415..12855)
                     /gene="yadI"
                     /locus_tag="ACHOWS_001679"
     CDS             complement(12415..12855)
                     /gene="yadI"
                     /locus_tag="ACHOWS_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0047262.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12960..13730)
                     /gene="yadH"
                     /locus_tag="ACHOWS_001680"
     CDS             complement(12960..13730)
                     /gene="yadH"
                     /locus_tag="ACHOWS_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0047263.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13727..14653)
                     /gene="yadG"
                     /locus_tag="ACHOWS_001681"
     CDS             complement(13727..14653)
                     /gene="yadG"
                     /locus_tag="ACHOWS_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0047264.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14762..15424
                     /gene="can"
                     /locus_tag="ACHOWS_001682"
     CDS             14762..15424
                     /gene="can"
                     /locus_tag="ACHOWS_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFQ0047265.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15465..16001)
                     /gene="hpt"
                     /locus_tag="ACHOWS_001683"
     CDS             complement(15465..16001)
                     /gene="hpt"
                     /locus_tag="ACHOWS_001683"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0047266.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            16207..18597
                     /gene="gcd"
                     /locus_tag="ACHOWS_001684"
     CDS             16207..18597
                     /gene="gcd"
                     /locus_tag="ACHOWS_001684"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFQ0047267.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILAWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKNLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18793..20343)
                     /gene="cueO"
                     /locus_tag="ACHOWS_001685"
     CDS             complement(18793..20343)
                     /gene="cueO"
                     /locus_tag="ACHOWS_001685"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFQ0047268.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20509..20856
                     /gene="yacC"
                     /locus_tag="ACHOWS_001686"
     CDS             20509..20856
                     /gene="yacC"
                     /locus_tag="ACHOWS_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFQ0047269.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20962..21828
                     /gene="speE"
                     /locus_tag="ACHOWS_001687"
     CDS             20962..21828
                     /gene="speE"
                     /locus_tag="ACHOWS_001687"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFQ0047270.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21844..22638
                     /gene="speD"
                     /locus_tag="ACHOWS_001688"
     CDS             21844..22638
                     /gene="speD"
                     /locus_tag="ACHOWS_001688"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFQ0047271.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(22676..23038)
                     /gene="yacL"
                     /locus_tag="ACHOWS_001689"
     CDS             complement(22676..23038)
                     /gene="yacL"
                     /locus_tag="ACHOWS_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFQ0047272.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(23213..25810)
                     /gene="acnB"
                     /locus_tag="ACHOWS_001690"
     CDS             complement(23213..25810)
                     /gene="acnB"
                     /locus_tag="ACHOWS_001690"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFQ0047273.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            26165..27922
                     /gene="yacH"
                     /locus_tag="ACHOWS_001691"
     CDS             26165..27922
                     /gene="yacH"
                     /locus_tag="ACHOWS_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFQ0047274.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQEVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPTNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHSAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSDHTVRLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(27993..29417)
                     /gene="lpdA"
                     /locus_tag="ACHOWS_001692"
     CDS             complement(27993..29417)
                     /gene="lpdA"
                     /locus_tag="ACHOWS_001692"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0047275.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29742..31634)
                     /gene="aceF"
                     /locus_tag="ACHOWS_001693"
     CDS             complement(29742..31634)
                     /gene="aceF"
                     /locus_tag="ACHOWS_001693"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0047276.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKDVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31649..34312)
                     /gene="aceE"
                     /locus_tag="ACHOWS_001694"
     CDS             complement(31649..34312)
                     /gene="aceE"
                     /locus_tag="ACHOWS_001694"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFQ0047277.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34473..35237)
                     /gene="pdhR"
                     /locus_tag="ACHOWS_001695"
     CDS             complement(34473..35237)
                     /gene="pdhR"
                     /locus_tag="ACHOWS_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFQ0047278.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(35693..35983)
                     /locus_tag="ACHOWS_001696"
     CDS             complement(35693..35983)
                     /locus_tag="ACHOWS_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0047279.1"
                     /translation="MYNFKDKVEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDKPENILKIVKEWRRSQGLSLFKDSE"
     gene            complement(35984..36223)
                     /locus_tag="ACHOWS_001697"
     CDS             complement(35984..36223)
                     /locus_tag="ACHOWS_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047280.1"
                     /translation="MSQYPELIAQFSSGNQTRIKQGLIAKAPLEGWHYGPKEIVKKFH
                     MYHCVAIEYSGEIYDIDNLRRAHLICMMKFITGDS"
     gene            complement(36381..36674)
                     /locus_tag="ACHOWS_001698"
     CDS             complement(36381..36674)
                     /locus_tag="ACHOWS_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0047281.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSTMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIVKEWRRSQGLPLFKDSKQSR"
     gene            complement(36677..>36868)
                     /locus_tag="ACHOWS_001699"
     CDS             complement(36677..>36868)
                     /locus_tag="ACHOWS_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0047282.1"
                     /translation="QTRIKQGLTAKAPLEGWRYGPKEIVKKFQIHHRVAIEYGGSVYD
                     IDNLRIVTPRLHDEIHYRR"
BASE COUNT         9347 a         9551 c         8721 g         9249 t
ORIGIN      
        1 cattatgcaa aatagccatc ccgatgatga atgaatgtaa tagttggtaa acaacagaca
       61 aacatcatta acacagtaac gattttgtta cgcagcattg cagcagactc ttatcgtcat
      121 gcggtgcggt tatctctcta tggaatatta ttcaaggatt tttatgtcta aaaaattagg
      181 ttttgcgctg tctggtatta tgttggcaat ggctgctggt actgcatttg ctgctaacga
      241 tatggacggt ggtcagttaa atattagcgg cctggtcgtt gataacacct gtgaagctcg
      301 tgttgatggc ggtaacaaag atggtttgat cctgctgcaa accgctactg ttgctgaaat
      361 caccgatggt gtactggata ccaccgtagg tgctaaagct aagccattca gcattactat
      421 tgactgcagc aaagctaacc cggctccggg tactactgca aaaatgactt tcggttccgt
      481 gttctttagc aatagcaaag gtactctgaa taacgatatg tctatcaata atccatctga
      541 tggtgtgaat attgccctgc ataatattga aggttccact attaagcagg ttcaggttaa
      601 taacccgggt gatgtttaca gcaaaactct ggatgcaacg tctaaaactg caacctacga
      661 ttttaaagca tcctatgttc gtgctgatgc aagcaaagca gcaactgccg gttatgtaaa
      721 aactaactca gcatatacca ttacttacca gtaaatatca gccacctaaa ttgattgggt
      781 gaatatatat agggaataac atcaaacata tttatttttg aacgttattc cctgttttaa
      841 ttaactctgt gaggaacggt ttatgttttt taatactaaa cataccgcag cattgtgctt
      901 agcgagctgt atggctttta gctcatcagc tattgcagac atcgtaattt caggtacacg
      961 cgtagtttat aaaagcgaac aaaaaagcgt caatgtgcgt ctggaaaata aaggcaataa
     1021 cccattgctg gttcagagtt ggttagatac tggagatgac aacgctgaac caggcagtat
     1081 tactgtcccc tttaccgcta cgccaccagt atcacgtatt gatgctaaac gtggccaaac
     1141 aatcaaactg atgtacaccg ccagtagtgc tctgccaaaa gacagagaaa gtgtgttctg
     1201 gtttaacgta ctggaagttc caccaaaacc cgatgcagca aaagcagcaa atcaaagcct
     1261 gctacaactg gcatttcgta cacgtataaa actgttctat cgcccggaag gattgagtgg
     1321 aattccatct gatgcgccat tggctctgaa gtggtcctgg gcaacatctg aaggcaaagc
     1381 ggcattacgc gttgataacc ctacgcccta ctatgtctct tttagcagtg gtgatttaga
     1441 agccaacgga aaacgctatc ccctggatgt gaaaatgatt gcaccgttca gtaacgacgt
     1501 catcaaggta aatgggatga gtggcagaac aagctctgca aaagtgcatt ttttcgccat
     1561 taatgatttt ggcggctcta ttgaaggcaa cgccaacctt taatctggca aggatatacc
     1621 acagcaggaa gcagaacgtg actattaaat ccactaaaaa atatcataat caccttaccc
     1681 gcatcgccac gttttgcgcg ctgctgtata gcaacagtgc tttatgcgct gaactcgtcg
     1741 aatatgacca tacatttctg atgggaaagg atgcttcaaa tatcgacctt agtcggtata
     1801 cagaaggcaa cccgacacta ccaggcattt acgatgtcag tgtgtatgtt aacgatcaac
     1861 ctatcatgag ccaaagcatt gcttttactg ttattgaagg taaaaagaac gctcaggcct
     1921 gcattacgca gaaaaattta ttgcaatttc atatcagttc accagacaag aatagcgaaa
     1981 aagccatttt gcttaagcga gacgaagatc ttggtgattg ccttaatctg gctgaaatga
     2041 tccctcaatc atccattcgt tacgatgtta acgatcaacg cctggatatc gacgtccccc
     2101 aggcatggat tatgaagaat tatcaaaact atgttgatcc ctctttgtgg gaaaatggca
     2161 ttaatgctgc catgctttcc tacaacctca acggatatca cagtgaaagt ccaggcagaa
     2221 caaatgacag tatctacgcg gcattcaacg gtgggataaa cttaggtgcc tggcggttac
     2281 gtgcctcggg taactacaac tggatgacca atgtccatag cgattacgat tttcagaatc
     2341 gctatttaca acgcgatctt gcgtcactac gttcacaact ggttattggt gaatcttaca
     2401 caaccggcga aaccttcgat tcagtcagta ttcgcggtat tcgcttatac agtgacagtc
     2461 gaatgctgcc tccggtatta gccagttttg cgccaatcat tcatggcgta gcgaatacca
     2521 atgccaaagt aaccgttatg caaaatggtt ataaaatcta tgaaacgacg gtgccgccag
     2581 gggcctttgc cattgatgat cttagcccgt caggttatgg cagtgatctc attgtgacta
     2641 tcgaagaagc tgatggcaca aaacggacgt tctcccagcc attttcatct gttgtgcaaa
     2701 tgctgcgccc tggcgttggt cgctgggata ttagcgccgg tcaggtttta aaagacagta
     2761 tccaggatga gcctaattta tttcaagcca gctactacta tggtttgaat aactacctga
     2821 caggatatac cggtattcag ctaactgata ataactatac cgccggattg ctggggcttg
     2881 gtatgaatac ccccgtgggg gctttttctg tcgatgttac tcactcaaat gtcagcattc
     2941 cggacgataa aacctatcaa ggacaaagct accgtatttc ctggaacaag ttatttgaaa
     3001 ataccagtac atcattaaat atcgcggcct atcgctactc cacccagcat tatctgggcc
     3061 tcaatgatgc gctgacgctg attgatgaag tagaacatcc tgagcaagat cttgagccta
     3121 aatccatgcg caattactcg cgcatgaaaa atcaggtcac ggtcagtatc aaccaaccgc
     3181 tgaaatttga gaaaaaggat tacggttcat tttatctctc gggaagctgg tcagattact
     3241 gggcctccgg gcaaaacagt actaattact ctattggtta tagcaatagt gcgtcctggg
     3301 gcagttacag tatcagtgct caacgttcgt tgaatgaaga tggtcaaacc gacgatagca
     3361 tttatctcag cttcacgatt ccaatagaaa atttactggg tacggaacac cgcagctccg
     3421 gcttccagag tattgataca caattaaaca gcgactttaa gggtaataac caactcaata
     3481 tcagcagtag tggttacagc gataccaatc gcatcagcta cagcgtaaac acgggttata
     3541 tgatgaataa atccagtgat gacctgagtt atatcggcgg ttatgccagt tatgaatcgc
     3601 cgtggggaac tctgtcaggt tcagcctcag caagcagtga taatagtcgc caattctcac
     3661 tcaatactga tggtggattt gtgttacata gcggcgggct gacgttcagt aatgacagtt
     3721 ttagtgattc cgacactctg gctgtcattc aggcgccagg agcaaaaggc gctcgtatta
     3781 actacggaaa cagcaccgta gaccgctggg gttatggtgt gaccagcgcc ctttcgccat
     3841 accatgaaaa ccgtatcgca ctggacatta acgatctgga aaacgatgtt gaattgaaaa
     3901 gcaccagtac tgtcgccgtc ccgcgccagg gagccgtcgt ctttgctgat tttgaaacag
     3961 ttcagggaca atcggcaatt atgaatatcg tgcgaagcga tggtaaaaat attccatttg
     4021 cagcggatat ttatgatgaa caaaacaata tcatcggcaa cgtaggtcag ggtggtcagg
     4081 cctttgttcg cggtatcggg caggaaggaa atatacgaat tacctggatt gaagagggca
     4141 aaccagtcag ttgttttgct cattaccaac aaaacacgac atcagaaaaa atagcgcaat
     4201 ctattattct gaacggactt cgttgtcaga tacagtaacc acaagggact tccaatgata
     4261 aaaaccacac cacataaaat agcaatactc atggggctat tattatcgcc atctgtattt
     4321 gcaacggatg ttaatgtcga ttttaccgct accgtgaaag cgacaacatg taacatcacc
     4381 ctgacatcgc tgaatggttc aaacgtcgtt gataatggca atgatagtta taccctgata
     4441 atccccaaga tgggactgga taagatagtc aataaagcgt cacaatctga ggccaacttc
     4501 aaattagtcg ccagtgggtg tagcagtgga attagctgga ttgataccac aatgactgga
     4561 aatcagtctg gtagtacatc gctgattatt ccacaatctt ctgactcctc ctcaacaacc
     4621 agtaatattg gtatgggatt taaaagatta gagacttcgg gcgatacgtt tctgaaaccc
     4681 aatagcgcgg aatacatccg ctggaaaacc acagaaatta gcaccagtgg tctggaaatg
     4741 actgtcgcac tgcgcgaaac aaccccagga cagggcatac ccgggaaatt tcgcgcacag
     4801 gcaacgttca atttcatcta tcaataacaa ggatgacaat gatgatatta aaaaaattat
     4861 gttatggatt atcgggtagt ctatttctgg cttcatcact gttaaccatt tcctcacatg
     4921 cggccactga tagtattggc ctgactgtta ctacagacat agaaatgggt acctgtacat
     4981 caacgttgac tgatgacggt acagctaaac tgtccaccat caatttcggt gatgtatata
     5041 tttctgaaat taatgccgaa actaaaatta aaaccttcaa attgcagttc aaagactgtg
     5101 cggggattcc cgggaaaaag gccaaaataa agttgacccc acgagcctta tgcgaaggta
     5161 attctaataa cggccctgga tttgctaacg catcaacggc aacagcaaaa gcagcggcag
     5221 tcgccgttga ggtctggagt acgtccacac caggtaaaaa cggcgcaaaa caattcagtt
     5281 gcgtcacccc cgcaacagaa gaagtcagta ttgctggtgc gacgggaggt gatgttgtcg
     5341 attacccgat gagtgctgtt ttggtggtcg cgaaagataa aaccgttaca gatgtcaccg
     5401 cgggtgattt caccgctccg gcaacattta ctgtgactta taactaacag gcagaccgat
     5461 tcaggaacta aatatgcgcc ccactcaacg taatctgatg aaaaaatcaa ttctgcttct
     5521 gtctttgcta ttcagcacca ctttccctgt ggttgcagga caggatgttg acattgttgc
     5581 aaatataaaa aataacacct gccaaagtgg aatcagcaac aacggaaata ttgatttagg
     5641 tgtcgttggc gtgggcgatt ttaccggaaa catttcagcc gaaaattatc accccggtgg
     5701 aaaagagttc actattacag tacaggactg cacacttcag ggcacaggga atgtgttaaa
     5761 tcagttacat attgattttc gcgcactcag tggcgtaatg gcgacaggtt ccagtcaaat
     5821 attcgccaat gaagacaaca ccggggctaa aaatgttggg gtagttatat tctctattca
     5881 ggacccggca aataccttca acgttcttgg cgtaactggt agctcacgtt ccgtttatcc
     5941 ggtcatgtca tcagcactaa acaattcatc ctggaaattt agcactcgaa tgcaaaaaat
     6001 cgatccagcg ctgaatgtca cctctggcca acttataagc catgtgttag tggatattta
     6061 ctacgaataa cagacaaagt gctgcaagca ttatcttgca gcatgacaat ggagtaaaaa
     6121 tgaagatatt cttcagatac tttctttttt tagcactatg ctcttgttgt tatacagcaa
     6181 gtgccggcac cgatgataac gtcagctatc ttgttgggaa tgattacgga gtgggtccgg
     6241 gagaccaaaa gtggagagaa acaggcccca gtgcagacat tactgtcaaa ttcaggtatt
     6301 caacaagcac aaataactta gttttttata aaccaaccca actcggacca acaggtgtgt
     6361 cattatactg gtctcagtta aatagcgctt caggtggagg ttacctttac tgcaacagaa
     6421 gtaacactgc aagcggtgag cctatggcaa ttgaaaatca aatggttgat tcgggtaaac
     6481 gttatggcgg gcataaacta tttgaaacat ctgtcccagg cctgtattat acattaaaga
     6541 tatcaaacat ttggtcatca tacaccacaa ccaatatcag ttcatcaggt atgtatatcg
     6601 gcgattctcc aactcaatat tttcggtgga tccccgaaag tgaagccact ctatattggt
     6661 cttgtaacaa tgcaaactca tctaataaat actgggccgt aggtggagtt tggcagtcgc
     6721 tcacggtgga attttatacc gataccaact ttgaccccac aaagacaaac aaggtaacat
     6781 tatctaagcc gggtggttat ctctactcat tcaaaggata tggtcttggt ggcggtatca
     6841 acgatcacag ctatttcctt aaaatagatt ttgacctgga taatatgacg ttatccaacc
     6901 caacgtgttt tactgctgca cttactggcc catctgtaag cggttcaaca gttaaaatgg
     6961 gagattatac cccagcgcaa ataaaaaatg gcgcaacagc ggttcctttt gatatcacgc
     7021 tacagaactg tattcgtgtt cgcaatatag aaacaaaact tatgtcaaac aaggtgggca
     7081 gtgtaagcaa agaacttctg gctaacacac tcacaggaaa taacgcagca aaaggtgttg
     7141 gtgtactgat tgaaggttta aaaaacacta aaagtgcaca aatggtactg aaaccaaatg
     7201 attcaacgtc tgtttataaa gattacgaaa cagaagatga cactaccgga ggaatttacc
     7261 cggataaagg aaacggtacc tcacaacctc ttcatttcca ggctacatta aaacaagacg
     7321 gtaatattgc tatagaaccg ggggatttta aagccaccag cacttttcag gttacctacc
     7381 cctgataaca ctattgcacc gccagacaat ggcggtgcga tacggataaa tcattgcccc
     7441 caccaatgtg actgataaaa tgcgagacag cacaacatca tttatcagga tacgctatga
     7501 aaccaaccac gattgcttca ttgcagaaat gcaagcagga caaaaaacgt ttcgcaacca
     7561 tcaccgctta cgactatagc ttcgcaaaat tgtttgccga agaaggactt aacgtcatgc
     7621 tggtaggcga ttcactgggc atgacggttc aggggcatga ctccacgcta ccggttaccg
     7681 ttgccgatat cgcctatcac accgccgctg tgcgtcgcgg cgcgccaaac tgccttctgc
     7741 tggctgacct gccatttatg gcgtatgcca cgccggaaca agcctttgaa aacgccgcaa
     7801 cggtaatgcg tgccggagcc aacatggtca aaattgaagg cggtgagtgg ctggtggaaa
     7861 ccgtacaaat gctgaccgaa cgtgccgttc cagtttgtgg tcatttaggc ctaaccccac
     7921 agtcagtgaa tattttcggt ggctacaaag tccaggggcg cggcgatgaa gcgggcgatc
     7981 aactgctcag cgatgcattg gcattagaag ctgctggggc acagctgctg gtgctggaat
     8041 gcgtgccggt tgaactggca aaacgtatta ccgaagcact ggcgatcccg gttattggca
     8101 ttggcgcagg caacgtcact gacgggcaga tcctcgtgat gcacgacgcg ttcggcatta
     8161 ccggcggcca cattcctaaa ttcgctaaaa atttcctcgc cgaaacgggc gacatccgcg
     8221 cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt ttacccgggc gaagaacaca
     8281 gtttccatta aggagtgacg ttgtgttaat tatcgaaacc ctgccgctgc tgcgtcagca
     8341 aattcgccgc ctgcgtatgg aaggtaagcg cgtggcgctg gtgcccacca tgggtaacct
     8401 gcacgatggc catatgaagc tggtcgacga agccaaagcc cgcgccgatg tggtagtggt
     8461 cagtattttc gttaacccga tgcagtttga ccgcccggaa gatctggcac gttatccacg
     8521 gaccttgcaa gaggactgcg aaaagctaaa caaacgtaaa gtggatttag ttttcgcccc
     8581 ttcggtaaaa gagatctacc cgaacggtac agaaacccac acctatgttg atgttcctgg
     8641 tctttccacc atgctggaag gtgccagccg tccgggacat tttcgcggcg tttcgacgat
     8701 tgtcagcaag ctgttcaacc tggtccagcc ggacatcgcc tgcttcggtg aaaaagattt
     8761 tcagcaactg gcgctgatcc gcaaaatggt tgctgatatg ggctttgata ttgagattgt
     8821 cggtgtgcca attatgcgcg ccaaagacgg tctggcgctg agttcccgta acggttatct
     8881 gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa gttttaagtt cgattgccga
     8941 caaattgcag gctggcgaac gggatctcga tgaaattatt actattgccg ggcaagaact
     9001 gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgt gatgccgaca ccttgctgga
     9061 ggtctctgaa aacagcaaac gtgcagtgat tctggtagcc gcctggcttg gcgatgcccg
     9121 cctgatcgac aacaaaatcg tcgaactggc gtaatcctta tctggcgcta cggctgatgg
     9181 cgccagttat tcattgccac gttatctgta ggcaattatt catatcgtag acaacttagt
     9241 tagagttaat tagcttatca atctctgcaa gagataaagc tgtcacctct gcaacgtctt
     9301 cccgagacat tcctttactc agaagacgct gggccagtct taccttctgg agtccctctt
     9361 tatatccctt ctgaaatccc ttctgaaatc ccttctcttc aaaccactgc gccaacgtct
     9421 gcctggtttc tctccgtgtc tttatttcca tatgttccct taattatctg gtcagttaac
     9481 aataacaatt atctggctat aactgataat gcagataact tagttaatca gcttatcaac
     9541 ctcagcaaga ggtaaatttg ccatctctgc aacgtcttcc cgagacatcc ctttactcag
     9601 aaaacgcagg gcgtattcct gtcttacttc ctgtctaacc tcttgtatta cctcctgtct
     9661 tacctcctgt cttacctcct gccttacctc ctgcctttcc tcctgtcttc ccttctcttc
     9721 aaaccactgc gccagcgtca tcatagactc ccctcccgtt tccctgtctc tcagcacacc
     9781 ataaaacaaa tccgcttgtt cagtatgacc gcgttgcagc atatagtttt gcatggcaac
     9841 taactgactt ccgctagtgt acccttcgtc aatcagcgtg accagttgct ccagcaataa
     9901 cattaagtcg cgctggcgaa tatgtttttg cagtagttcg agaatcgcaa tccgccgatg
     9961 ttgcatgatt tcgtcatccg gtgtgatggt gatatccacc agcgggaaag ggttgttgta
    10021 aacgcgtcgc gccagctccg gcgagtaaaa catatcaaac cagcacattg agagcggata
    10081 aggtgtggcc tcgccctgat aaaacagaat cggcaccacc agcggcagct tatcgtgatc
    10141 cgcctccaga tgccggtgca tggcggcaat ggcatagcgc atcatgcgaa aggtcatttt
    10201 cttatccggc ttgctttgat gttcaatcac cacatgcaaa taaccgggat taccctgcat
    10261 ttgcacggaa taaagcacgt cagtgctgtg ccctttcagg ctttcttcaa taaaactccc
    10321 cgactcaaga tgcagcgtgt cgaggtcaca cagttcgcgt agttccgctg gcaaatggat
    10381 atccagaaag tcacgagccg tttccgcatg cattaaaaat tgtttaaata ccgcgtcatg
    10441 cggtgtggta ctcggtgcat ccatcttccc tgccttccgt gttgtcttgc gtgaaggcag
    10501 tatccagtca aggcgcgccg tcgtcagccg cctctttatt gtttaccgag cagcgttcag
    10561 agaaatttca attggttaca ggcgttacat ggcacgcttc ggcacggtac gcaaactgct
    10621 gatgcaaatg ccgtcagatg agtagacact aaacaaaaat cgggcaatac tgcgtgagaa
    10681 tttcctcaaa gcaggcatcg cctgcttcgt taacgacagg gtagaaaggt agaagttatg
    10741 attcgcacga tgctgcaggg caaactccac cgcgtgaaag tgactcatgc ggacctgcac
    10801 tatgaaggtt cttgcgccat tgaccaggat tttcttgacg cagctggtat tctcgaaaac
    10861 gaagccattg atatctggaa tgtgaccaac ggcaagcgtt tctccaccta tgccatcgcg
    10921 gcagaacgcg gttcgagaat tatttccgtt aacggtgcgg cggcccactg cgccagcgtc
    10981 ggcgatattg tcatcatcgc cagcttcgtt actatgccag atgaagaagc tcgcacctgg
    11041 cgacccaacg tcgcctattt tgaaggcgac aatgaaatga aacgtaccgc gaaagcgatt
    11101 ccggtacagg ttgcctgata atcacccctg cggctggtta ctcaccagcc gcgacatcgt
    11161 ctcgagcgaa tccgttctta agatataaag tcgttttagt aacaacggat tatcccccgg
    11221 ttttactttg cctttcatgg ttgtcaccgc caggtgaaat ccggcttctt ttgctgcctt
    11281 cacggctttg tcattaaatc cgccaaacgg atacgaaaga taccagacat gcggattaaa
    11341 ttgcgccaga gcgcggcgtg aacgtgcaaa atcaaacaga atattgtgct cactacggct
    11401 cagcaatatg ggtcggcgat aaccatccac ccgatgcaaa aaatgggtat gtgactggaa
    11461 atcaaataca tcgcgaattt cgttaagttc agaaacgctc ataaattgca gcgattttgg
    11521 gtcccacttc tgcgggtgac gtttgatgcg cgaggtaaca ataaacgccg tcgccttcat
    11581 gccatattgt ttcaacacag gatacgcata gcggctcacc gatttgaggc catcatcaaa
    11641 ggtaatcacc accgctcgcg cagggagatt gatcttattc ttcacgtaac cttccagctg
    11701 caccatactc agtgtcgtgt atcccctgtc gcgcagccag gccatctggt tattgaaagc
    11761 gcgtaccgag gtggtcgtcg aagtatggcg aaaacgggtg ttttcttcgt cgcgcagaat
    11821 atggtgatag gttagcaccg gcaggccatt atcgggttgg gcatccagcg cgctgatata
    11881 ggccagtcga tcgccaatac ggatctgata ccaggtttga tttaacctgt ctttcagttt
    11941 atgcaaaatc gggtagcgca aattgtccgc cagtacccca aacggcgcac tgcccgcact
    12001 cggcgcgtta tagaccggcg tatctttcca ggtaactaag ttctgattac tcagcggctt
    12061 gttgaggtca cccaaaccgt cttcaacttt ttgtcgcccc tgaaccggct cgagatgacc
    12121 tttatcgata aaccctttac caaagccaaa attaaatgcg taataacttg cggcagtggg
    12181 ctccacggca atgatttgcc cggcccgaat attccccacg gtcaccatct tgtcgccaat
    12241 ttgcgcccag accgcagcat tttcgatggt ttgcatataa cgggcaggta acgcggcact
    12301 gacgcttgcg gtaaacagca tcagcaggag aataacagct tgtttgtaca tgaaaaaata
    12361 accgagggtg aaacaacgct tttattgtag caaaagcgtc atcagaatcg cgggttaata
    12421 ctcatacaaa ttatgtttga ggacgaaagg cggatttttt tgttgctggt gccaaagact
    12481 actcacctcc ggcgcaccca gttcgacaat acgctcgcga aactctgcgc tggataacgt
    12541 ttcacggtag accagcatct gctcaattaa cggtaacgtg acgccagaaa tcacttcgca
    12601 acgggaatgt ttatggctta atagcgaagc cacgcggtac ggtggcgctc cggctatatc
    12661 ggttaagaag atgacacctt caccagagtc cgcttcatgc agagcatcgc acatcatgcg
    12721 gctgagcata ttagagctta acccacgcca gaaattcacg gcccggcact gaagaagtgc
    12781 cccatgtttt ttctccagcg catccagtat ctcttgcgcc cggtcatcgt gacaggtaat
    12841 tacccaacct aacatcgcct tcctccttag ccgaaaatca gtctaccctg accattgttg
    12901 ataaagggtg atagccgtca aaactcctct cccccaaatc cagaggagag gaaatagcct
    12961 tagctacgca aaccacgtcc acgttggatc agcgaccaac agatcaaata aaacgccaca
    13021 ataaagacca ccagtacacc gaaggtagtg accagcggaa catcattgat accgaggaag
    13081 ccgtagcgga aaccactgat catataaacg attgggttca ggtgcgacag cccttgccag
    13141 aacggcggca acaaagtcag tgagtaaaag accccgccca aatacgtaag tggcgttaac
    13201 acaaaggttg gcaccaggct gatgtcatcg aacgttttgg caaacacgcc gttcagcaaa
    13261 cccgcaaggg agaacaacac cgccgtgagc accagcgtta aagcgacaaa tacccacgaa
    13321 tgcacctgaa acggcacaaa aaacagtgaa attgccgtca ccagaatgcc aacaaacaga
    13381 ccacgcgcca cgccaccgcc gacatatccg gcaataatga cgtgagtcgg aaccggcgct
    13441 accagcagct cttcaatatt acgctgaaac ttggcaccaa aaaatgatga cgcgacgtta
    13501 gcgtaggcgt tggtgatgac cgacatcatg atcagccccg gtacaatgaa ctgcatgtag
    13561 ctgaagccat gcatatcgcc aatacgcgaa ccaatcaggt taccgaagat aataaagtaa
    13621 agggtcatgg tgatgaccgg tggcaccagc gtctgcaccc agatacgcat aaagcgatgg
    13681 atctctttcg cccagatgct ttttagtgcc acccagtaaa gatgcatcat gcgcgatctc
    13741 cttgtttttc attaaccagt gaaacaaaca gctcttccag acggttagct ttgttacgca
    13801 tacttaatac ctgaatgccc tgctcactta actgcgtaaa tacgctgttg atcccctgct
    13861 cacgcagaac ttcaacttcc agcgtcgcgg tatcgaccag tcgatactga tagccatcga
    13921 gcttcggtaa cgggcttttc ggtgcgagat cgagaataaa ggtttccgat ttcagcttcg
    13981 ccagcagcgc cttcatcgag gtattttcca ccagctcacc gtgttgaata atgccgatat
    14041 tgcggcacag catttctgct tcttccaggt agtgtgtggt gagaatgata gtggtgcctt
    14101 tgtcgtttaa atccttcaaa aagccccaca ttgagcggcg aagttcgata tccacgcctg
    14161 cggtcggttc gtcgagaatc agtagtttag gttcatgcat taacgcacgg gcaatcatta
    14221 aacggcgctt catcccgcca gataacatac gcgcacgttc gttgcgtttt ccccatagat
    14281 cgagttgttt aagatacttt tcgctgcgaa tgtacgcttc tttgcgctcc acaccgtagt
    14341 accctgcctg attcaccaca atttgctgca cggtttcaaa cgggttgaag ttaaattcct
    14401 gtggcaccag acccaactga cgtttagcgt ttacgacatc tttctcgaga tcgtaaccaa
    14461 atacgctgac ccgcccggag gttttattta ccagagagct gataataccg atagtggtcg
    14521 attttccggc cccgttcggc ccgagaagcg cataaaaatc acccgcttcg acctgcaaat
    14581 cgattccacg aagcgcctga acgccgcctg gataggtttt tttaagctgt tgaagttcca
    14641 gtgcaatggt cataaatttt tacttacctt acgttcttac actttatctg tggtttaaat
    14701 cgtcccggag ttgccctata ttagccaaac gtaattattt ggttacaggt cgttaacctc
    14761 catgaaagac atagatacac tcatcagcaa caatgcacta tggtcaaaaa tgctggtgga
    14821 agaggatccc gggttttttg agaaactggc acaagcgcaa aaaccgcgct ttctatggat
    14881 tggatgttcc gacagtcgcg ttcctgcaga acgtttaacc ggtcttgagc cgggcgaact
    14941 ctttgttcac cgtaatgttg ctaacctggt cattcacacc gacctgaact gcctttccgt
    15001 ggttcagtat gcagtggatg tactcgaagt tgaacacatt attatctgtg gccactacgg
    15061 ttgcggcggc gtacaagccg cagttgaaaa cccggaactg gggcttatca acaactggct
    15121 gctgcatatc cgcgatatct ggttcaaaca tagctcattg ctcggcgaaa tgccgcaaga
    15181 gcgccgtctg gataccttgt gtgaactgaa cgtcatggaa caggtgtata acctgggcca
    15241 ctccaccatt atgcaatcag cgtggaaacg cgggcagaaa gttaccattc acggctgggc
    15301 ctacggcatt cacgacggct tgctgcgtga tctggatgtc acagcgacta accgcgaaac
    15361 ccttgagcaa cgttaccgtc acgggatctc caacctcaag ctgaaacacg ccaaccacaa
    15421 ataaaaatgc catgccggat gcaacacatc cggcaacttc acacttactc gtccagcaga
    15481 atcactttgc cgatatacgg cagatgacgg taacgctgtg cgtaatcaat gccgtaaccc
    15541 accacaaact catccgggat cgagaaaccg ataaattcta ccgggacgtt cacttcacga
    15601 cgggacggtt tatccagcag cgtacaaatc gccaacgact tcggttcgcg caggcttaag
    15661 atctcacgca ctttcgacag tgtattcccc gagtcgatga tatcttcaac aatcagcacg
    15721 tccttgccac ggatatcttc atccagatct ttgaggattt tcacatcacg ggtggtggac
    15781 atgccgctac cgtagctgga ggcggtcata aaatcgactt catgagatac ctgaacttca
    15841 cggcacaggt ccgccataaa cataaatgag ccacgcagca gaccaaccag caccatatcg
    15901 ctgccactgt ctttgtaacg ctcagtaatc tgacgaccca gttcggcgat acgcgcttta
    15961 atctccgctt cggggatcat tacttctaca gtatgtttca tatctctaac catatgattt
    16021 aaaaacaaat cacttcgcac gggcaggttt cagcaccgca acaaagcgca agacacgcag
    16081 tataccagta aaactattca ttcgcggaat ctgttaataa agctcgaatt gtgatgacga
    16141 tcacacatgt taaaacctat aattaattgc tactaaaata ttaatgaatt gaaatggtgt
    16201 ctctttatgg caattaacaa taccggctcg cggcgattac tcgtcacgct aacagccctt
    16261 tttgcagcgc tttgcgggct gtatctactc attggcggag gctggctggt cgcgattggc
    16321 ggctcctggt actaccctat cgctggcctt gtgatgctcg gcgtcgcctg gatgctgtgg
    16381 cgcagtaaac gcgccgcgct ttggctgtac gcagccctgc tgctcggcac tatgatttgg
    16441 ggcgtctggg aagttggttt cgacttctgg gcgctgactc cgcgcagcga cattctggtc
    16501 ttcttcggca tctggctgat cctgccgttt gtctggcgtc gcctggtcat tcctgccagc
    16561 ggcgcagttg ccgcactggt ggtggcactg ctgattagcg gtggtatcct ggcctgggcc
    16621 ggatttaacg atccgcagga aatcagcggc accttaagcg ccgataccac gccagcagaa
    16681 gccatctccc ccgttgccga tcaggactgg cctgcctatg gtcgcaatca ggaaggtcaa
    16741 cgcttttcac ctctgaaaca aattaacgct gataacgtcc acaagctgaa agaagcctgg
    16801 gtgttccgta ctggcgatgt gaagcagccg aacgatccag gtgaaatcac caatgaagtg
    16861 acgccaatta aagtgggcga caccctttac ctgtgtaccg ctcaccagcg cctgtttgcg
    16921 ctcgatgccg ccagcggcaa agagaaatgg cattacgatc ctgagctgaa aaccaacgag
    16981 tccttccagc atgtaacctg ccgtggcgtc tcttatcatg aagccaaagc agaaactgct
    17041 tcgccggaag tgatggcgga ttgcccgcgt cgtatcattc ttccggtcaa tgatggtcgt
    17101 ctgattgcaa ttaacgctga aaacggcaag ctgtgcgaaa ccttcgccaa taaaggcgtg
    17161 ctcaatctgc aaagcaatat gccggacacc aaaccgggtc tgtatgaacc gacttcgccg
    17221 ccgattatca ccgataaaac catcgtgatg gccggttcag tcaccgataa cttctcaacc
    17281 cgtgaaacat ctggcgtgat ccgtggtttt gatgtcaaca ccggggagct gctgtgggct
    17341 tttgatcctg gtgcgaaaga tcctaacgca atcccgtctg acgaacacac ctttaccttt
    17401 aactcgccaa actcgtgggc accagcggcc tatgacgcga agctggatct ggtgtatctg
    17461 ccgatgggcg tgaccacgcc ggatatctgg ggcggtaacc gcacaccgga acaggaacgt
    17521 tatgccagct cgattctggc gctgaatgcc acaaccggga aactggcgtg gagctaccag
    17581 accgttcacc acgacctgtg ggatatggat cttccggcac agccgacgct ggcggatatc
    17641 accgttaatg gtcagaaagt gccagttatt tatgctccgg cgaaaaccgg caacattttt
    17701 gtgctcgatc gtcgtaatgg cgaactggtg gttccggcac cggaaaaacc ggttccccaa
    17761 ggtgctgcca aaggcgatta cgtaacccca actcaaccgt tttctgaact gagcttccgt
    17821 ccgacgaaaa atttgagcgg tgcggatatg tggggagcca ccatgtttga ccagctggtg
    17881 tgccgcgtga tgttccacca gatgcgctat gaaggcattt tcaccccgcc atctgaacag
    17941 ggtacgctgg tcttccctgg taacctgggg atgttcgaat ggggcgggat ttccgttgat
    18001 ccgaatcgtg aagtggcaat tgccaaccca atggcactgc cgtttgtttc gaaactgatc
    18061 ccacgcggtc ctggcaaccc gatggagcag ccgaaagatg ccaaaggcac gggtacggaa
    18121 tccggcattc agccgcagta cggtgtaccg tatggcgtca cgctcaatcc gttcctctca
    18181 ccgtttggtc tgccgtgtaa acagccagca tggggttata tctcggcgct ggatctgaaa
    18241 accaatgaag tggtgtggaa gaaacgtatt ggtacgccgc aggacagtat gccattcccg
    18301 atgccggtac ctgtgccgtt caatatgggt atgccgatgc tgggcgggcc aatctccacg
    18361 gcgggtaacg tgctgtttat cgccgctacg gcagataact acctgcgcgc ttataacatg
    18421 agcaacggtg aaaaactgtg gcaaggtcgt ttaccagcgg gtggtcaggc gacgccaatg
    18481 acctatgaag tgaatggtaa gcagtatgtg gtgatctccg caggcggtca cggttcattt
    18541 ggtacgaaga tgggcgacta tattgtggct tatgcgttgc cggatgatgt gaagtaaggc
    18601 ttgctcagat tgctgacaac gtgcgatttg ttcatgccgg atgcggcgtg aacgccttat
    18661 ccggcctaca aaacatttgc aaattcaata cattgcgcgt aggcctgata agcgtagcgc
    18721 atcaggcagt tttgcgtttg tcatcagttt catgcccgga gagatccggg catatttccg
    18781 aatacggtct ttttataccg taaaccctaa catcatcccc gtatcttcat gctccagcaa
    18841 atggcagtgc gccatataag catgttcttt cggtgcatcg tgattaaact tcaccagcac
    18901 ttcgctgaca ttaccttcta ccttaacggt atctttccag cctgcgcgat gcgtcgctgg
    18961 cggtttgcca ttttctgaca agatacggaa ctgcgtaccg tggatatgga acggatgcag
    19021 catcatgtcg cccacgccag agataaccca acgttcgtat tgccctttcg ccgccgcaaa
    19081 catcggcttg ttcatatcaa acgcctgacc gttgatttta ttggcatggt ggaaatcgaa
    19141 cttcccgccg tggttcatat gattcatatt gccgtgcccc atatggccca tcatctggct
    19201 gtgatccatc ccggccatcg cctgatcgcc atatttctcc atcagcatct gcatccccat
    19261 catatcgagc attgggtcca tagaaagttg cagcttgcgt accgtcagcc cttccagcga
    19321 aggtaacgca ggcaggctac ttaatgtgtc tggcaaagca ccagaggcac taatggcaat
    19381 cggctgaatc cgcattaccg gatgcggctt gtcaaacggc gcaatcgcca tccccatctg
    19441 gctgaccggc agcgtcacca ggtcaaaggg tttgttgtcg ttaacctcca ccagcacttc
    19501 aaaacgctcg cccatcagca ctggcagctc gttcaccttc accggttcag gtagcagacc
    19561 gccgtcgctg gcaatcacat aaagcgggcg attgtcgctg gtggcgaaat tgagcgagcg
    19621 ggcattacag ccattgagca aacgcaggcg cagccagcca cgcggggcag cgtgttgtgg
    19681 gtagattaca ccgttggtca gcaacgtatc gccaaaccaa cccacagcgg cggtcatcac
    19741 atccagttga taatcaatct gcccgtcagc gttaaatttt ttatcctgaa caataaccgg
    19801 aacatcatca ataccccact gttttggcag cattaatttc aggatctcgt catcttcaat
    19861 caccaccaaa ccagccagcc ccatcgccac ctgacgtccg gtcttgccat gttgatgtgg
    19921 atggaaccag caggtagcgg caggttgatc aacgttcaac gtcaccgagc gcttgccacc
    19981 tggcggaata attccctgcg ggccgccgtc cacttcaccc ggtacttcca gcccgtgcca
    20041 gtgcaacgtc gtctcttccg tcagttggtt gtagatatca accgttaccg ctttgccgcg
    20101 ctgtaatttc accgccggcc ccagcagatt gccgttatag ccccaggtag ttgcggtttt
    20161 cccgccaaag gtggactgac ctgcgccaat agttaactga atgcgattac gggcatcggt
    20221 cgtgagcaaa tcagggatcg gtaacgttgg gcgttccgcc gcaaatactg cgcggctcca
    20281 cagcggcaaa gccgaagcca cgcccagcgc gacggaatat tttaagaaat cacgacgttg
    20341 catagttatt tccttattct taagcaggcg aaacaaaatc aaacgttgag catagtcctt
    20401 ccccttacgg gaaggtcaag ccgcagacac aatttaatcg acaataatgg tcgtcgcctc
    20461 gctcgcagtg tgctaacgtt tatcttcttt aagcccctgg tagaagcaat gaagacgttt
    20521 ttcagaacag tgttattcgg cagcctgatg gccgtctgcg caaacagtta cgcgctcagc
    20581 gagtctgaag ccgaagatat ggccgattta acggcagttt ttgtttttct gaagaacgat
    20641 tgtggttacc agaacttacc taacgggcaa attcgtcgcg cactggtctt ttttgcccag
    20701 caaaaccagt gggatctcag taattacgac accttcgaca tgaaagccct cggtgaagac
    20761 agctaccgcg atctcagcgg cattggcatt cccgtcgcta aaaagtgcaa agccctggcc
    20821 cgcgattcct taagcctgct tgcctacgtc aaataatccc tgataccttt ttgtagaaat
    20881 attgaccgtg catccgcggt caatgttagc tattatgttg cgcccttttt ttacgggtgt
    20941 taacaaagga ggtatcaacc catggccgaa aaaaaacagt ggcatgaaac gctacacgac
    21001 cagtttgggc agtactttgc ggtagataac gttctgtatc atgaaaagac cgatcaccag
    21061 gatctgatca tttttgagaa cgccgcattt ggtcgcgtaa tggcgctgga tggcgtagta
    21121 caaaccaccg agcgcgacga gtttatctat catgagatga tgacccatgt tccgctactg
    21181 gcccacggtc acgcgaaaca tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt
    21241 gaagtaaccc gacataaaaa cgttgagtca atcacgatgg tggaaatcga tgcgggtgtc
    21301 gtgtcgttct gccgtcagta tctacccaac cataacgccg gtagctacga cgatccgcgc
    21361 tttaaactgg tgatcgacga tggcgtcaat ttcgttaatc aaaccagcca gacctttgat
    21421 gtcattattt ccgactgcac cgatcctatc ggtcccggcg aaagtctgtt cacctcagca
    21481 ttttatgaag gctgcaaacg ttgcctgaat cctggcggta tcttcgtcgc acaaaacggc
    21541 gtctgctttt tacagcagga agaggccatc gacagccatc gcaaactcag ccattacttc
    21601 agcgatgttg gcttttatca ggcggcgatc ccgacctatt acggcggcat catgaccttt
    21661 gcatgggcga cagataacga cgccttacgc catctctcaa ccgaaattat tcaggcgcgt
    21721 tttctcgctt ctggcctgaa atgccgttat tacaatccgg cagtccatac ggcagctttt
    21781 gccttacccc agtatctgca agatgcactg gcttcacagc cgtcctaagg agaagataag
    21841 aaattgaaaa aactgaaact gcatggcttt aataatctga ccaaaagtct gagtttttgt
    21901 atttacgata tctgctacgc caaaactacc gaagagcgcg acggttatat tgcttatatc
    21961 gatgaactct ataatgccaa ccgtctgacc gaaatcctgt cagaaacctg ttccattatc
    22021 ggggccaata ttcttaacat cgcccgccag gattacgagc cacaaggtgc cagcgtcact
    22081 attctggtga gtgaagaacc ggtcgacccg aaacttatcg acaaaacaga acaccccggc
    22141 ccactgccag aaacggtcgt tgcccatctc gataaaagtc atatttgcgt acatacctac
    22201 ccggaaagtc atcctgaagg cggtttatgt accttccgcg ccgatattga agtctctacc
    22261 tgcggcgtga tttctccgct gaaggcgctg aattacctga tccaccagct tgagtccgat
    22321 attgtgacta ttgattatcg cgtgcgcggt tttacccgcg acattaacgg tatgaagcac
    22381 tttatcgacc atgagattaa ttcgattcag aactttatgt ctgacgatat gaaggcgctg
    22441 tatgacatgg tggatgtaaa cgtctatcag gaaaatatct tccataccaa gatgttgctt
    22501 aaagagttcg accttaagca ctacatgttc cacaccaaac cggaagattt aaccgacagc
    22561 gagcgccagg aaattaccac tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc
    22621 aatatgcccg ctgtttaacg tctcaggcgg agcacggagg ctccgcctga tttacttact
    22681 tctgctgcac gaaattacgg taagccgcca ccacctgaag aaaatcctca acgccgcaca
    22741 gcgacaagct ctcttcatcg tagtagttca tcccctcttc catttcatcg ccagcgaatt
    22801 ccagttgatt ggcgcgaacc atcacctctt caccatccat ccatagcgtg tattcatgcc
    22861 ctgcccgttg ccaggaacgt tcgctacctt tcaatgcgtg cgccgcttgt tctacttcat
    22921 caagcaaggc caggttttct ttcacctctt cattaaacca gtgcccgacc acttcatgcc
    22981 ccatggacat gcgcaccttt accactccgg taatatcgcg cagaaattcg taatccatag
    23041 tgtgttcctc tgctcctggc aatgcgccga aatcgcattg cgccattgat gtaattatcg
    23101 cagccttacc ggagaaaaac agcggaacgg cgatggttta gaaataaaaa atgcccggga
    23161 atctcattca ccgggcattg tgtcgtttat gcgcagcgcg tgcgcttact tattaaaccg
    23221 cagtctggaa aatcaccccg tccgcttttt cggtgtactg agaaagctgg tcgaagttca
    23281 gataacggta agtatcaacg gctgttttat ctacctgcgc cacgtaggtc tggtactctt
    23341 ccggcgtcgg cagtttgcca atcagcgccg caacagcagc cagttccgca gaagccagga
    23401 agacattcgc gccagtaccc aaacggttcg ggaagttacg ggtagaggtg gaaaccaccg
    23461 tcgcaccgtc tgctacacgc gcctggttac ccatacacag ggaacagcca gggatctcga
    23521 tacgagcacc actcttaccg aagacgctgt agtagccttc ttcggtcagc tgcgcagcgt
    23581 ccatacgcgt tggcggtgcc acccacaggc gggtcggcaa ctgacctttg tgcgcatcca
    23641 gcagtttacc agcagcacgg aagtgaccga tgttagtcat gcaagaaccg ataaatactt
    23701 cgtcgatctt ctcgccctgt accgcagaca gcggacgtgc gtcgtccggg tcattcggtg
    23761 cacagaggat tggctctttg atatccgcca gatcgatgtc gatcactgcc gcgtattccg
    23821 catctgcatc ggcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac
    23881 gacgctccag ggtacgacga tcgccgtaac cttccgcgat catccacttc agcaggacga
    23941 tgttagagtt caggtattca atgatcggtt ctttgttcag cttgatggta caaccggcag
    24001 cagaacgctc ggcagacgca tcggtcagct caaatgcctg ctcaactttc agatccggca
    24061 gaccttcaat ttccagaatg cggccagaga agatgttttt cttacctttc ttctcaacgg
    24121 tcagcagacc ttgtttgatt gcatacagcg ggatcgcgtg taccagatcg cgcagggtga
    24181 tacccggctg cattttgcct ttgaagcgca ccagaacgga ttctggcata tccagcggca
    24241 ttacgcctgt tgcggctgca aatgccacca gaccagaacc cgccgggaaa gagataccga
    24301 tcgggaaacg ggtatgggag tcaccaccgg taccgacagt atccggcagc agcatacggt
    24361 tcagccagga gtggattacg ccgtcacccg gacgcagcga cacaccgcca cggttcataa
    24421 tgaagtccgg cagcgtgtgg tgcgtgttaa cgtcaactgg tttcggatac gccgcggtgt
    24481 ggcagaaaga ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt
    24541 catcacgggt catcgggccg gtggtgtcct gggaacctac agaagtcatt ttcggttcac
    24601 agtacgcgcc cggacgaatg cctttcacgc cacaggcacg gcctaccatt ttctgcgcca
    24661 gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg acggaacaca tcgctgtgcg
    24721 gcaggccaag tgcttcacgc gctttggtgg tcaggccacg cccaataatc agcggaatac
    24781 ggccgccagc acgcacttca tcaatcagca cgtcggtttt cagttcgaag gtcgccagca
    24841 gttcgccggt ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca attacgtcgc
    24901 ccatgttcag gttagagacg tcgacttcga ttggcagcgc acccgcgtct tccatcgtgt
    24961 taaagaagat gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca
    25021 catgtggaat atcatcgccc ataaaccaca gaacggagtt agtggcggat ttacgcgatg
    25081 accccgtacc cacaacgtca ccgacgtacg ccagtgggaa acctttctgt tgcagagctt
    25141 cgatttgctt aatcggacca acaacgcctg gctgatctgg ctcaatacct tcacgggcgt
    25201 ttttcagcat cgccagcgcg tgcagtggga tatccgggcg tgaccacgca tccggtgccg
    25261 gagagaggtc atcggtgttg gtttcgcctg tgactttgaa gacggtaacg gtcagttttt
    25321 cagccagcgc cgggcgattc aggaaccatt cggcatccgc ccaggactgc attacctgct
    25381 tcgcatattc gttgcctgct ttcgctttct cttctacgtc atagaagtta tcgaacatca
    25441 gcagcgtgtg agaaagtgct ttggcagcga tcggtgccag tttggcatca tccagcgcgt
    25501 cgatcagcgg atgaatgttg taaccaccct gcatggtgcc cagcagttcg atggcttttt
    25561 ccggagtcag cagaggggat ttggcttcgc ctttagcgat agcagccagg aagcctgctt
    25621 ttacataggc ggcttcatcg acgcctgggg gaacacggtt ggttaacaga tctaacagga
    25681 attcttcttc gcccgcgggc gggtttttca gcagctctac aagcgcggcc atttggtttg
    25741 catccagggg tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt
    25801 cttctagcac gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca
    25861 cgctcctgtg agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc
    25921 aggattttga cgatgtgtta atctgtttac aaaaaagcaa cataaaaaag tggctgaatc
    25981 gttaaggatg gtctagagat tgtttccctc cgtaaaaact tcgttaattc gcatggtgat
    26041 aatcaccttt ctcaacgaat cccaagatta gtcaaaattt aaactaccgc ctctttatac
    26101 tcggattcac agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg
    26161 aattatgaaa atgactttgc cgtttaaacc ccatgtgctg gcgctaattt gcagtgccgg
    26221 gctttgtgcc gcctctaccg ggctatatat aaaaagccgc acagtggaag cgcctgtgga
    26281 accgcaatcg acacaactgg ctgcgcctga catctccgca gttacgcttc ctgcaacggt
    26341 ttccgtgcct cctgtaacac ctgccgtcgt caaatccgca ttcagcactg cgcaaataga
    26401 tcaatgggtt gcacctgtcg cgctataccc cgactctctg ctttcacaag tgttaatggc
    26461 atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata atccacttaa
    26521 acaaggcgat gctgctattc aggcggtatc tgaccagccc tgggacgcca gcgtcaaatc
    26581 actggtggcc tttccacagt tgatggcatt gatgggcgaa aacccgcaat gggtgcaaaa
    26641 cctgggcgat gcttttctgg cgcagccgca ggaggtgatg gactcggtac agcgattgcg
    26701 gcaactggcg caacaaaccg gctcgctgaa gtcatcaact gaacagaaag ttattaccac
    26761 aacgaagaaa gctgtaccgg taaaacagac agtcacggct cccgtcatac catccaatac
    26821 cgtttcaact gccaaccctg tcattacaga gcctgcaaca accgtcattt ccattgaacc
    26881 caccaatcct gatgtggtct atattcccaa ctacaaccca accgtggttt acgggaactg
    26941 ggccaatact gcgtatccgc cggtttatct gccgccgcca gccggagaac cgtttgttga
    27001 cagctttgtg cgcggattcg gctatagcat gggcgttgct accacgtacg cgctattcag
    27061 cagcatcgac tgggatgacg acgatcatga ccatcatcat catgacgatg atgattatca
    27121 ccatcacgat ggcggtcatc gtgatggtaa tggctggcaa cacaatggcg acaacatcaa
    27181 tatcgacgtc aacaatttca accgtatcac cggtgaacat cttactgata agaatatggc
    27241 atggcggcac aatccaaact accgtaatgg tgtgccctat catgatcagg atatggcaaa
    27301 gcggtttcat caaaccgatg tcaacggcgg aatgagcgcc acgcagctac ctgctccaac
    27361 acgcgacagc cagcgtcagg cggcagcaag tcagtttcag caacgaacac acagcgcacc
    27421 agtcattaca cgagataccc aacgtcaggc agcggcacag cggtttaatg aagctgaaaa
    27481 ctatgggagc tatgacgact tccgcgactt cagccgtcgc caaccactga cccagcaaca
    27541 aaaggacgcc gctcgtcagc gttatcagtc ggcctctcct gagcagcgcc aggcagtccg
    27601 cgagaaaatg cagactaacc cgcagatcca gcagcgaaga gaggcagcgc gtgagcgcat
    27661 tcagtctgca actcccgaac agcgccaggt gtttaaggaa aaagtacagc agcgcccact
    27721 gaatcaacag caacgtgata acgcccgcca gcgtgttcaa tcagcatcac ctgagcaacg
    27781 tcaggttttt cgggagaaag ttcaggagag ccgcccgcaa cgtctaaacg acagtgacca
    27841 tactgtcagg ctgaataacg agcaacggtc agcagtacgc gaacgtctct ctgagcgcgg
    27901 agcaaggcga ctggaaaggt aaattacaga cgtaaaaaaa gcggcgtggt tagccgcttt
    27961 tttaattgcc agatgttccg gcaaacgaaa aattacttct tcttcgcttt cgggttcggc
    28021 aggtcggtaa tgctaccttc gaacacttct gccgccaggc ccacagactc gtgcagagtc
    28081 gggtgcgcat ggatggtcag tgcgatgtct tcagcgtcac aacccatttc gattgccagg
    28141 ccgatttcac ccagcaactc gccgccgtta gtgccgacaa tcgcaccacc gatcacacgg
    28201 tgagattctt tgtcgaaaat cagcttggtc ataccgtctg cgcagtcgga agcgatagca
    28261 cgaccagaag cagcccacgg gaaggtggcg gtttcgtagc tgatgccttt ctctttcgct
    28321 tctttctcag tcagacctac ccatgcaact tctggttcgg tataggcgat ggacgggata
    28381 actttcggat cgaagtaatg ttttttaccg gcgataactt cagcggcaac gtgaccttcg
    28441 tgaacacctt tgtgtgccag cattggctga ccgacgatat caccgatagc aaagatgtgc
    28501 ggtacgttgg tacgcagctg tttgtcaacg cggatgaaac cacggtcgtc aacttccacg
    28561 ccagctttgc ctgcgtcgag gtttttaccg ttcggcacac gaccaatcgc taccagcacg
    28621 gcgtcgtaac gctgcggttc agcgggtgct tttttgcctt ccatcgtcac ataaatgccg
    28681 tcttctttcg cttcaacggc ggtaactttg gtttccagca tcaggttgaa tttcttgctg
    28741 atacgcttgg taaagacttt aacgatgtct ttatcagctg ccgggatgac ctggtcgaac
    28801 atttcaacca cgtcaatctg tgaacccagc gcgtggtata cggtgcccat ttccagaccg
    28861 atgataccgc cacccattac cagcaggcgt tctggtactt ctttcagttc cagcgcgtca
    28921 gtggagtccc agatacgcgg atcttcatgc ggaataaacg gcagttgaat cgggcgagaa
    28981 cccgctgcaa tgatcgcgtt gtcgaagttg atcacggttt taccgttctc accttcaact
    29041 tccagggtgt tagccccggt aaatttaccc agaccgttga ccactttgac tttgcggcct
    29101 ttcgccatac cagccagacc accggtcagc tgattgatca ctttctcttt ccaggtacga
    29161 atcttgtcga tatcggtttt cggttcgcca aagacgatac cgtgttcagc cagcgctttg
    29221 gcttcttcga taacttttgc tacgtgcagc agtgctttag aagggataca gccgacgttc
    29281 aggcaaacac cgccaagggt gttgtagcgt tctacgatta cggtttccag acctaaatca
    29341 gcgcaacgga aggcagcgga gtaacctgcg gggcctgccc caagtaccac gacctgagtt
    29401 ttgatttcag tactcatcat gacctctata tatttatctc cggcggtcat acccgtcgtc
    29461 tttcaggccg cagcggcgtt agcttcacgc cctcacccca gtcacttact tatgtaagct
    29521 cctggggatt ccggcgcttg ctaccttgct gcacccagaa atccataggg tatgtgaagt
    29581 aataccctaa ccaccaccgg gtcgttctat ccgtcggtat tttacaaaat tgttaacaat
    29641 ttttaaacaa caaacggcaa ccgatttgtc tattcgctaa taacctcaat acattcatga
    29701 gattaccaga aaaaagccgg ccgttgggcc ggctctttta cttacatcac cagacggcga
    29761 atgtcagaca gcgtgttgtt aatgatggta atgaaacggg caccatcagc accgtcgatc
    29821 acgcggtggt cgaaggagag agaaatcggc agcatcagac gcggcacgaa ctctttacca
    29881 ttccacaccg gctccatcgc ggacttggaa acgccgagga tagccacttc cggcgcgttc
    29941 acaatcggcg cgaagtgggt agtacccagg ccgccgatgc tggagatggt gaagcaaccg
    30001 ccctgcattt cgcccgcagt cagcttaccg tcacgcgctt tcttagaaat agtcatcagc
    30061 tcgcgagaca gctcgatgat gcctttcttg ttgacgtctt tgaataccgg aacaaccaga
    30121 ccgttcgggg tatccaccgc cacaccgatg ttgatgtatt tcttcagggt cagacgctga
    30181 ccgtcttccg acagcgaact gttgaagcga ggcatctgct caagagctgc agcaacggct
    30241 ttcatgatga agacaaccgg ggtgatcttc acatccagct tacgtttcgc cgcttcttcg
    30301 ttctgctgtt tacggaacgc ttccagctcg gtgatatcgg ttttgtcgaa gtgggtaaca
    30361 tgcgggatca tcacccagtt acggctcagg ttcgcaccag agattttctg gatacggccc
    30421 agttccactt cttcgatttc accaaacttg ctgaagtcca ccttcggcca cggcagcatg
    30481 cccgggatac cgccgccagt cgccgccgga gccgcttctg cacgtttgat agcttctttc
    30541 acgtaagcct gaacgtcttc gcgcaggata cgacctttac ggccagtgcc cttcactttc
    30601 gccaggttaa cgccaaattc gcgtgccaga cggcggatca gcggagtcgc gtgaacgtaa
    30661 gcgtcgtttt cagcaaattc agatttgcct tcggctttcg cagccggagc tgctgctgga
    30721 gcttcagctt ttgctgccgg agccggcgct gccgcttcct gtttcgcagg agctgccgca
    30781 ggcgctgcgc cttcaacttc gaagatcata atcagcgagc cagttttcac tttatcgcca
    30841 acgttgactt tcagttcctt cacgacgcct gcaaacggag ccggaacttc catagacgct
    30901 ttatcgcctt ctacggtgat cagtgactgt tcagcggcaa ctttgtcgcc cactttcacc
    30961 atcacttcgg tcacttcaac ttcgtcaccg ccgatatccg gaacgttaac gtctttcacg
    31021 ccagccgctg gtgcagaggc cgctgccgga gccgcttcct gtttagccgc cggagctgcc
    31081 gcgcctgctt cacccgcgac ttcgaagacc ataatcagcg agccggtaga cactttgtca
    31141 cccacgttca ctttgatctc tttcacggtg ccagcaaacg gagccggaac ttccatagaa
    31201 gctttatcgc cttctacggt gatcagcgac tgttcagctt caactttatc gccaactttc
    31261 accaggattt cggtcacttc aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt
    31321 gccgctgcag ccgctggagc tgctgccgga gcggcttctt tcttctcttc tgcctgagca
    31381 ggtgcagcgt ctgctgcacc gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg
    31441 gttttatcgc caacagagac tttgatctct ttaacgatac ccgcctgcgg agacggaact
    31501 tccatagagg ctttgtcgcc ttctacggtg atcagcgact gttcggcttc aactttgtcg
    31561 cccactttga ccaggatctc ggtgatttca acttcatcag ccccgatgtc cggtactttg
    31621 atttcgatag ccattattct tttacctctt acgccagacg cgggttaact ttatctgcat
    31681 cgatgttgaa tttggcgatt gcgtcagcaa ccactttctt atcgatttcg ccacgtttag
    31741 ccagttcgcc cagcgccgca accacgacat aagaagcatc aacctcgaag tggtgacgca
    31801 ggttctcacg gctgtcggaa cgaccgaagc catcagtacc cagtacgcgg tagtcgtcag
    31861 ccggtacgta agtacggact tgctcagcga acagtttcat atagtcggta gatgccactg
    31921 ccggagcgtc gttcatcacc tgagcgatat acggaacgcg cggagtttcc agcgggtgca
    31981 gcatgttcca gcgttcacaa tcctgaccat cacgcgccag ttcggtgaag gaagtcacgc
    32041 tataaacgtc agaacctacg ccgtaatctt tcgccaggat ctctgctgct tcacggacgt
    32101 gacgcaggat agaaccggag cccagcagct gaactttacc tttgctacct tcaatggttt
    32161 cgagtttgta gataccttta cggatacctt cctcagcacc ttccggcatt gctggcatgt
    32221 ggtagttttc gttcagcgta gtgatgtagt agtaaacgtt ctcttgtttc tcaccgtaca
    32281 tacgctccag accgtcatgc ataatgacag caacttcgta agcgtaagcc gggtcgtaag
    32341 agatacagtt cgggatagtc agcgactgaa tgtggctgtg accatcttcg tgctgcagac
    32401 cttcgccgtt cagggtggta cgaccggaag taccgccgat caggaagcca cgcgcttgct
    32461 ggtcaccagc cgcccagcac agatcgccga tacgctggaa gccgaacatc gagtaataga
    32521 tgtagaacgg aatcatcggc agattgttgg tgctgtaaga ggtcgccgct gccagccagg
    32581 aacacccagc gcccagctcg ttgatccctt cctgcagaat ctgacctttc tcgtcttctt
    32641 tatagtaagc aacctgctcg cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa
    32701 taccaatctg acggaacaga ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg
    32761 gtaccagacg atctttgatc gacttgttct tcagcattac gttcagggca cgaacgaaag
    32821 cgatagtggt agagatctct ttgctctgct cttccagcag cgcgccgaag tcttgcaggc
    32881 tcggcagctc aagcttctcg gtgaagttcg gctgacggct tggcagataa ccgtgcagtt
    32941 tctgacgctg agcgtgcaga taggtatgct cttcagaacc ttccgggaag gtgatgtacg
    33001 gcaatttttc gatatcggca tcagacaccg gcacattgaa acggtcgcgg atgtgacgca
    33061 cgccgtccat gttcattttc ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc
    33121 ccatgccgta acctttaatg gtatgagcaa ggattaccgt cgctttgcct ttggtttcct
    33181 gcgctttctt aaatgcagcg tagattttct tcggatcgtg accaccacgg ttcagtgccc
    33241 agatctgttc gtcagtccag tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga
    33301 agtgttcacg aacgtacgca ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg
    33361 tttcgttcat cagctggatc agtttaccgc tggtatcttt acgcagcagt tcatcccaac
    33421 ggctacccca catcactttg atcacgttcc agccagcacc ttcgaagatg ccttccagtt
    33481 cgttgatgat cttgccgtta ccggtgaccg ggccgtcaag acgctgcagg ttacagttga
    33541 taacgaaaac caggttatcc agtttttcac gggtagcgat ggtgatcgca cctttggatt
    33601 ccggttcgtc catttcaccg tcgccgagga acgcgtaaac ggtttgttta gaggtatctt
    33661 tcaggccacg gtgttccaga tatttcagga atttagcctg gtaaatagca ccaatcggcc
    33721 ccagacccat agatacggtc gggaactgcc agaattccgg catcagtttc gggtgcggat
    33781 aggaagagag gccattgccg tgaacttcct gacggaagtt atccagctgc tcctgagtca
    33841 gacgaccttc caggaaagca cgagcgtaaa cgcccgggga gatgtggccc tggaagtaaa
    33901 ccaggtcgcc gccatcctgc tcgttgcgtg cacggaagaa gtggttaaag cacacatcat
    33961 aaatggttgc ggaagactgg aaggacgcca tgtggccgcc cagttcgagg tcttttttcg
    34021 acgcacgcag aaccgtcatg atggcgttcc agcggatagc tgaacgaata cggcgttcca
    34081 gttccagatt acccggatac tccggttgtt cttcaacggg gatggtgttg atgtagttgc
    34141 tgataccggt gcctgcggct acgttgacac cgcctttgcg ggcttcagca agcagttggt
    34201 cgatcagata ctgagcacgc tcaacacctt cttcacggat gaccgattcg atcgcctgga
    34261 gccagtcgcg agtttcgatc ggatccacgt catttgggaa acgttctgac atgggttatt
    34321 ccttatctat ctaataacgt tgagttttct ggaacctgtc ccattgaact ctcgccggaa
    34381 agctcaataa gacaggttct acgtttagtt gccgcgcttt tatatgcgct tgatttacaa
    34441 catcttctgg ataattttta ccagaaaaat cactaattct ttcgttgctc cagacgacgt
    34501 agagaacgct cacggcggct ctcttcacgg cttctgtcga gcaaaatttc ttcgataaag
    34561 gccagatggc gatgcgatgc ttcgcgcgct tcttccggct taccggccat aatcgcttca
    34621 aatatgcggg tgcggtgact actcaccagc ggcagcatct cgcgacgcga atagagcaat
    34681 tcgaagttct ggcggacatt ctgggccaac atcggctcca tacaccttag cagatgaagc
    34741 agaaccacat tgtgggccgc ttcggtgacg gcaatctgat actggagtac ggcatttgat
    34801 tccgcgtcca gatcgccaga ctgctgcgcc agctctatgg catggtggag ttcacggatg
    34861 cgttccttgt cttcatcggt actacgcagc gcggcgtaat aagcggcgat accttccagg
    34921 gcgtgtcgtg tttcgagcaa gtcatactgt gattcaggat ggtcggagag cagctccacc
    34981 agcggatcgc tgaagctttg ccataggctg ctctggacaa aagtgccgcc accctggcga
    35041 cgaagcaaca agcccttcgc ttcgagacgt tgaatcgcct cacgcaagga gggacgggag
    35101 acgtcgaact gttttgccag ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg
    35161 ccttcgagga tcaaaaactc cagttgctgc tcaatcacat cggagagttt tggttggcgg
    35221 attttgctgt aggccatgag ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac
    35281 caatttcatg tctgtgacgc taaagtaaca aagtattcac cttatgtcca tacaggtttt
    35341 gattgaaatc atgaaactgt gcacatttta acaacttgac atatataacg tttcaaagtt
    35401 gtaactatgc acaaatgtta aatttaccca tcaaggggtg agattaacag aattgaaacg
    35461 aaaaaatatg tcctctgaac cgattcacca agcaacaaaa cgcataacaa ttcaattatg
    35521 tgaattaata tgtaaaatga atgttcaggg aaggagagaa aggtagtatt tacctatgtt
    35581 ttctttttac attgcttatt atcactgcaa tcgccaccgt aaccttctcg tagcgaaaat
    35641 catcacttaa ccagaacgtg atgattttca ggtgacagat tctgtttaga attcattctg
    35701 aatctttgaa caaagacaat ccttgcgaac gccgccattc tttgacgatt ttcaggatgt
    35761 tttcaggctt atcatctccc ttctttgcag gatagtagat aagatcgctc atcaacggat
    35821 gttgtgtaat tctggtaaga tgttcttcta gatcatccca atatttgtca agctcttccc
    35881 cttttaattg agcgttatcc ccggttggat ttgtaaactc gccaagcaac tcaatgaatt
    35941 cccgctctgt gtaatcttct actttatctt taaaattata catttaacta tctcctgtaa
    36001 taaatttcat catgcaaatg aggtgtgccc tccttaaatt atcaatatcg taaatttcgc
    36061 cactatattc aatcgcaaca caatgataca tgtggaattt tttaacgatt tctttagggc
    36121 cataatgcca accttcaaga ggtgcttttg cgattaatcc ttgttttata cgtgtttgat
    36181 taccacttga aaactgcgct atcaactccg gatactgact cacctccagc caaagggtat
    36241 cggtgaaagc gggaaattga taaaataacc taatgagatt tcagtatttt acctgttttt
    36301 taagagcacc cacattatta ttttatgtta ttaacatcat cgcattagcc agacgcgatg
    36361 atgttccata tatagtttta ttatctactt tgtttagagt ctttaaacaa gggtagcccc
    36421 tgtgaacgcc gccattcttt gacaattttc aggatgtttt caggcttatc atctccttga
    36481 cttttagggt agaaaattag atcgctccct gacggatgct gtgtaatttc aataaaatga
    36541 tctactaatg aatcccagta catttctagc tccttacctt ttaagggctt tccatctttc
    36601 atagttgatc tcaatccgcc taaaaaatta agaaattcct cctcggtata atcttcgagc
    36661 ttttctttaa acatatttat ctcctgtagt gaatctcatc gtgtagtctg ggggtaacaa
    36721 ttcgtaaatt atcaatatcg tatacgctgc cgccatattc aattgcaaca cgatgatgta
    36781 tctggaattt tttaacgatt tctttagggc cataacgcca accttcaaga ggtgcttttg
    36841 cggttaatcc ttgttttata cgtgtttg
//
LOCUS       DAZKJR010000033        36369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKJR010000033 DAZKJR010000000
VERSION     DAZKJR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.033"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            314..1687
                     /gene="aroP"
                     /locus_tag="ACHOWS_001700"
     CDS             314..1687
                     /gene="aroP"
                     /locus_tag="ACHOWS_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFQ0047283.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTCFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1730..2584)
                     /gene="ampE"
                     /locus_tag="ACHOWS_001701"
     CDS             complement(1730..2584)
                     /gene="ampE"
                     /locus_tag="ACHOWS_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFQ0047284.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQIDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVLVVIAL
                     LTIYGALV"
     gene            complement(2581..3132)
                     /gene="ampD"
                     /locus_tag="ACHOWS_001702"
     CDS             complement(2581..3132)
                     /gene="ampD"
                     /locus_tag="ACHOWS_001702"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFQ0047285.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLVAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3220..4113
                     /gene="nadC"
                     /locus_tag="ACHOWS_001703"
     CDS             3220..4113
                     /gene="nadC"
                     /locus_tag="ACHOWS_001703"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFQ0047286.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4316..4756
                     /gene="ppdD"
                     /locus_tag="ACHOWS_001704"
     CDS             4316..4756
                     /gene="ppdD"
                     /locus_tag="ACHOWS_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFQ0047287.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4766..6151
                     /gene="gspE"
                     /locus_tag="ACHOWS_001705"
     CDS             4766..6151
                     /gene="gspE"
                     /locus_tag="ACHOWS_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFQ0047288.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAISFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVENPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAINAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6141..7343
                     /gene="hofC"
                     /locus_tag="ACHOWS_001706"
     CDS             6141..7343
                     /gene="hofC"
                     /locus_tag="ACHOWS_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFQ0047289.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALFPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTE
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7378..8421)
                     /gene="guaC"
                     /locus_tag="ACHOWS_001707"
     CDS             complement(7378..8421)
                     /gene="guaC"
                     /locus_tag="ACHOWS_001707"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0047290.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSFEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8577..8621)
                     /locus_tag="ACHOWS_001708"
     CDS             complement(8577..8621)
                     /locus_tag="ACHOWS_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047291.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8646..9266
                     /gene="coaE"
                     /locus_tag="ACHOWS_001709"
     CDS             8646..9266
                     /gene="coaE"
                     /locus_tag="ACHOWS_001709"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0047292.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     PREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9266..10009
                     /gene="zapD"
                     /locus_tag="ACHOWS_001710"
     CDS             9266..10009
                     /gene="zapD"
                     /locus_tag="ACHOWS_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFQ0047293.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPVVDYAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10019..10216
                     /gene="yacG"
                     /locus_tag="ACHOWS_001711"
     CDS             10019..10216
                     /gene="yacG"
                     /locus_tag="ACHOWS_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFQ0047294.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10418..10816)
                     /gene="mutT"
                     /locus_tag="ACHOWS_001712"
     CDS             complement(10418..10816)
                     /gene="mutT"
                     /locus_tag="ACHOWS_001712"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFQ0047295.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHLSLFEILEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(10876..13581)
                     /gene="secA"
                     /locus_tag="ACHOWS_001713"
     CDS             complement(10876..13581)
                     /gene="secA"
                     /locus_tag="ACHOWS_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0047296.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13643..14155)
                     /gene="secM"
                     /locus_tag="ACHOWS_001714"
     CDS             complement(13643..14155)
                     /gene="secM"
                     /locus_tag="ACHOWS_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFQ0047297.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14386..15303)
                     /gene="lpxC"
                     /locus_tag="ACHOWS_001715"
     CDS             complement(14386..15303)
                     /gene="lpxC"
                     /locus_tag="ACHOWS_001715"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFQ0047298.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15404..16555)
                     /gene="ftsZ"
                     /locus_tag="ACHOWS_001716"
     CDS             complement(15404..16555)
                     /gene="ftsZ"
                     /locus_tag="ACHOWS_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0047299.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16616..17878)
                     /gene="ftsA"
                     /locus_tag="ACHOWS_001717"
     CDS             complement(16616..17878)
                     /gene="ftsA"
                     /locus_tag="ACHOWS_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0047300.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17875..18705)
                     /gene="ftsQ"
                     /locus_tag="ACHOWS_001718"
     CDS             complement(17875..18705)
                     /gene="ftsQ"
                     /locus_tag="ACHOWS_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFQ0047301.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18707..19627)
                     /gene="ddlB"
                     /locus_tag="ACHOWS_001719"
     CDS             complement(18707..19627)
                     /gene="ddlB"
                     /locus_tag="ACHOWS_001719"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0047302.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDANPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19620..21095)
                     /gene="murC"
                     /locus_tag="ACHOWS_001720"
     CDS             complement(19620..21095)
                     /gene="murC"
                     /locus_tag="ACHOWS_001720"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0047303.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21149..22216)
                     /gene="murG"
                     /locus_tag="ACHOWS_001721"
     CDS             complement(21149..22216)
                     /gene="murG"
                     /locus_tag="ACHOWS_001721"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0047304.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22213..23457)
                     /gene="ftsW"
                     /locus_tag="ACHOWS_001722"
     CDS             complement(22213..23457)
                     /gene="ftsW"
                     /locus_tag="ACHOWS_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFQ0047305.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23457..24773)
                     /gene="murD"
                     /locus_tag="ACHOWS_001723"
     CDS             complement(23457..24773)
                     /gene="murD"
                     /locus_tag="ACHOWS_001723"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0047306.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAVTGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24776..25858)
                     /gene="mraY"
                     /locus_tag="ACHOWS_001724"
     CDS             complement(24776..25858)
                     /gene="mraY"
                     /locus_tag="ACHOWS_001724"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0047307.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25852..27210)
                     /gene="murF"
                     /locus_tag="ACHOWS_001725"
     CDS             complement(25852..27210)
                     /gene="murF"
                     /locus_tag="ACHOWS_001725"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0047308.1"
                     /translation="MISVTLSQLTDILNGELKGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVNRPLDIDLPQVIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27207..28694)
                     /gene="murE"
                     /locus_tag="ACHOWS_001726"
     CDS             complement(27207..28694)
                     /gene="murE"
                     /locus_tag="ACHOWS_001726"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFQ0047309.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28681..30447)
                     /gene="ftsI"
                     /locus_tag="ACHOWS_001727"
     CDS             complement(28681..30447)
                     /gene="ftsI"
                     /locus_tag="ACHOWS_001727"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFQ0047310.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30463..30828)
                     /gene="ftsL"
                     /locus_tag="ACHOWS_001728"
     CDS             complement(30463..30828)
                     /gene="ftsL"
                     /locus_tag="ACHOWS_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0047311.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30825..31766)
                     /gene="rsmH"
                     /locus_tag="ACHOWS_001729"
     CDS             complement(30825..31766)
                     /gene="rsmH"
                     /locus_tag="ACHOWS_001729"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0047312.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31768..32226)
                     /gene="mraZ"
                     /locus_tag="ACHOWS_001730"
     CDS             complement(31768..32226)
                     /gene="mraZ"
                     /locus_tag="ACHOWS_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0047313.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32828..33832)
                     /gene="cra"
                     /locus_tag="ACHOWS_001731"
     CDS             complement(32828..33832)
                     /gene="cra"
                     /locus_tag="ACHOWS_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFQ0047314.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33914..34000)
                     /locus_tag="ACHOWS_001732"
     CDS             complement(33914..34000)
                     /locus_tag="ACHOWS_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFQ0047315.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34012..34503)
                     /gene="ilvN"
                     /locus_tag="ACHOWS_001733"
     CDS             complement(34012..34503)
                     /gene="ilvN"
                     /locus_tag="ACHOWS_001733"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFQ0047316.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34506..36230)
                     /gene="ilvI"
                     /locus_tag="ACHOWS_001734"
     CDS             complement(34506..36230)
                     /gene="ilvI"
                     /locus_tag="ACHOWS_001734"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFQ0047317.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
BASE COUNT         8714 a        10185 c         8929 g         8541 t
ORIGIN      
        1 gtggctaagg catagttttc agcaggcaga caaatccggc atatagctag ttaaaacagt
       61 atgttatcag acttaaagta tctattaaat gtgatgaaaa acttacaatt gctttttttt
      121 tatccaaaaa atcaacgcat ctacataaag caggtgcatt cccgggcaca taccattatc
      181 ttgtgatagt taaaagtagc attatcataa ttcagttaca tgaaacgtaa agaaatcaat
      241 acaacaaacg gaattgcaaa cttacacacg catcactgcg tagatcaaaa aaacaatcac
      301 cgcacgaggt ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc gcggtcttaa
      361 aaaccgccat attcagctta tcgcgctggg tggcgcgata gggaccgggc tattcctggg
      421 tagcgcctcc gtaatacagt ccgcagggcc agggattatc ctgggttacg ccattgctgg
      481 ttttatcgcc tttctgatca tgcgtcagct aggtgaaatg gtggtcgaag aacctgtcgc
      541 aggctccttt agccactttg cttataaata ctggggcagt tttgccggtt tcgcctctgg
      601 ctggaactac tgggtactgt acgttttagt tgccatggct gagctgactg ccgtgggtaa
      661 atacattcag ttctggtatc cggaaatccc aacctgggtt tctgccgccg tgttctttgt
      721 ggtgattaac gccatcaacc tgaccaacgt taaagtgttt ggcgagatgg agttctggtt
      781 tgccattatc aaagttatcg cggtggtagc gatgatcatc ttcggcggct ggctgctatt
      841 cagcggcaac ggcggcccgc aggcgagcgt tagcaacctg tgggatcagg gtggtttcct
      901 gccgcacggc ttcaccgggc tggtgatgat gatggcgatt atcatgttct ctttcggcgg
      961 tctggaactg gtggggatca ccgcagcaga agctgataat ccggagcaaa gtatcccgaa
     1021 agcaactaac caggttatct accgcatcct gattttctat attggttcgt tagccgttct
     1081 gctctcgctg atgccatgga cccgcgttac tgctgatacc agtccgtttg tactgatctt
     1141 ccacgaattg ggcgatacct ttgtggctaa tgcgctgaac atcgtggtac tgactgctgc
     1201 gctctccgtg tacaacagct gcgtatattg caacagccgt atgctgtttg gtctggcaca
     1261 acagggcaac gcgccaaaag cgctggcgtc tgtcgacaaa cgcggtgtac cggttaacac
     1321 tattctggtg tctgcgctgg ttacggcatt gtgcgtgctg attaactacc ttgcaccgga
     1381 atccgcattc ggactgttaa tggcactggt ggtatctgca ctggtaatca actgggcgat
     1441 gattagcctg gcgcatatga aattccgtcg cgccaagcag gaacaaggcg tggtaacttg
     1501 cttccctgct ctgctttatc cgctgggtaa ctggatctgc ctgctgttta tggcggcggt
     1561 actggtgatt atgctgatga ccccaggaat ggcgatttcg gtatacctga tcccggtatg
     1621 gctgatcgtg ttaggtatcg gctatctgtt taaagagaaa accgccaaag ccgtaaaagc
     1681 gcattaatct ctctacgccc tcacccgtgc agggtgaggg caataatctt tacaccaacg
     1741 ccccgtaaat cgtcagtagc gcaattacca ccaggaccac gaacgaggtt ttcttcgcca
     1801 tggaaaccgc tgccttcggc gtctccacct tatcgacatg cggttcacgc gccagagaga
     1861 actgtgccag acgtgttaat acctgatatt gcgaagtatg gaaatcaccc agcgaagcaa
     1921 accaggccgg taacgctttc tccccatgac cgatcaaggc atataccaca cccgcaagac
     1981 gaaccggcac ccaatccagt acatgaagca cggcatcaat gccggactgt aaacgatgat
     2041 gcggcgtctg gtaacgcgcc agccagtatt gccatgcacg caaaaacgca taccccatca
     2101 gcgtaacggg tccccaggtt ccccccacaa tcagccagaa cagcggtgca agataaaaac
     2161 gaaagttaat ccacagcaat gcattttgca gctcacgcaa atactcacgt tcatcgcagc
     2221 ctgccgggac gccgtgaatc atggtgagtt cgccagccat cgtggcacgg gcatggctat
     2281 cattacgtga agcagctgtc agataagcat gataatgaag acgaacttta cctgcgccaa
     2341 tacacagcaa accaatcagc agccacacca gcagcgtggg aacgttgaac aatactccct
     2401 gcaatgcgcg taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg cctaatgtgc
     2461 gcccgagaga aaaatgtttc acccgccgaa agaacgcttc aagacgatga tcaatctgcc
     2521 agtgctcgcc caacttaaac aggcgctcga aaattaacac cagtaaggtt gtaaatagcg
     2581 tcatgttgtc tccttgctga ccagtgcacg aaaccgtgcc caatcaaatg cagggccggg
     2641 atcggttttc cgatccggcg caatatcaca atggcccgtc atgttattag cgatatctgg
     2701 atagcgatca atcagtgccc gcgtaaccgc cacaagctgt tgatactgcg catcggtata
     2761 cgccagcgta tcggtgcctt ccagctcaat cccaatagaa aaatcattgc agcgttcgcg
     2821 cccctgatac tgagagactc cggcatgcca ggcacgttta tcgaaaggaa catactggac
     2881 tatttcacca tcgcggcgaa tcaaacagtg agcggagacg cgcaaatggg cgatctcagc
     2941 aaagaaagga tgcgcctgcg gatcaatagt tccagtgaat aatgcgtcga tccacggacc
     3001 gccaaactcg cctggcggca ggctaatatt gtgcaccacc agcagggtgg gtgtttcgtc
     3061 atccgggcgg caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca gccacccctg
     3121 ttctaacaac atgcaggatc tccttatatg tggtgctaat acccggttca gagtagcatg
     3181 tttctacctt atgattcgtt agctatctgg agttttaaca tgccgcctcg ccgctataac
     3241 cctgacaccc gacgtgacga gctgctggaa cgcattaatc tcgatatccc cggcgcggtg
     3301 gcccaggcgc tgcgggaaga tttaggcgga acagtcgatg ccaacaatga cattacggca
     3361 aaacttttac cggaaaactc tcgctctcat gccacagtga tcacacgcga gaatggcgta
     3421 ttttgcggca aacgctgggt tgaagaggtg tttattcaac tggcaggcga cgatgtcacc
     3481 ataatctggc atgtggatga cggcgatgtc atcaacgcca atgaaccctt gttcgaactt
     3541 gaaggcccat cccgcgtgct gttaacgggc gaacgcactg cacttaattt tgtgcaaacc
     3601 ctttcagggg ttgccagtaa ggtacgccac tatgtcgaat tactggaggg caccaacacg
     3661 cagttgttgg atacgcgcaa aaccttaccc ggcctgcgtt cagctctgaa atacgcggta
     3721 ctttgcggcg gcggagcgaa tcaccgcctg gggctttctg atgccttcct gatcaaagaa
     3781 aaccatatta ttgcctccgg ctcagtgcgc caggcggtcg aaaaagcgtc ctggctgcac
     3841 ccagatgcgc cagtagaagt cgaagtagag aatctggaag aacttgatga agccctgaaa
     3901 gcaggtgccg atatcatcat gctggataac ttcgaaacag aacagatgcg cgaagccgtc
     3961 aaacgcacca acggcaaggc gctactggaa gtgtctggca acgtcactga caaaacactg
     4021 cgcgaatttg ccgaaacggg cgtggacttt atctccgtcg gtgcgctaac taaacacgtg
     4081 caggcactcg acctttcaat gcgttttcgc tgattgctga cagatttatc cgctccatcg
     4141 cgggcggata atctcccctt tcccaacttt cttcgtaacg cctcgcaaat ttcttgtctt
     4201 tcgctgcaac gtaataaaaa actccggaag ctgctttccc gtttcgtttt ttgcccctcg
     4261 cttgtgctga tggcgtttgc caaagtagcg ccaaccaaat caaggagcga aacaaatgga
     4321 caagcaacgc ggttttacac ttatcgaact gatggtggtt attggcatca ttgccatttt
     4381 aagcgccatt ggtattcccg cttatcaaaa ctacctgcgc aaagccgcac tcaccgacat
     4441 gctacaaacc tttgtgcctt accgtaccgc cgtagagttg tgcgcgctgg aacatggtgg
     4501 attagatacc tgcgacggtg gcagcaatgg cattccctcg cctaccacca cccgctatgt
     4561 ttcagccatg agtgtggcaa agggtgtggt gtcgctgacc gggcaagaaa gtctcaatgg
     4621 gctaagcgtc gtcatgacac cgggttggga taacgcaaac ggcgtcaccg gctggacgcg
     4681 caactgcaat attcaaagtg acagcgcatt gcagcaagcc tgcgaagatg tctttcgctt
     4741 tgatgacgcc aactaaggag cggcaatgaa tattccacag ctcaccgccc tgtgtctgcg
     4801 ttatcaggga gtcttgctgg atgccagcga agaggtggtt catgttgcgg tggtcgatgc
     4861 cccttcgcat gagctactgg acgcattgca tttcgctacc accaaacgta ttgagatcac
     4921 ctgctggacg cgccaacaaa tggaaggtca cgccagtcgc acccaacaga cattgcccgt
     4981 agctattcat gagaagcatc agcccaaagc agagttgctg actcgaacgt tacaatctgc
     5041 gctggaacaa cgcgcgtctg atattcatat cgaaccagcg gacaatgcct accgcatccg
     5101 cttgcgtatc gacggtgtat tgcatccttt accggatgtt ttaccggatg ccggagtcgc
     5161 attaaccgcc agattaaaag tgctgggcaa cctggatatt gcggaacatc gcctgccgca
     5221 ggacgggcaa ttcactgtcg aactggcagg aaacgccatc tcatttcgta ttgcgacctt
     5281 accatgtcgg ggtggtgaaa aggtggtatt aaggttgtta cagcaggtga gtcaggcact
     5341 ggatgtcaac acgcttggaa tgcagccgtt acaactggcg gactttgctc atgccttgca
     5401 acaaccacag ggactggtgc tggtaacagg ccctacaggc agcggcaaaa cggtcacgct
     5461 ttatagtgcc ctgcaaacgc tgaataccgc tgacattaat atttgtagcg tcgaaaatcc
     5521 ggttgagatc cccatagccg gactaaacca gacgcaaatc catccgcgtg ccggactcac
     5581 ctttcagggc gttttacgtg cgttattgcg ccaggatcct gacgtcatta tgatcggaga
     5641 gatccgcgat ggcgaaacgg cagaaattgc cattaacgcc gcacaaacgg gtcatctggt
     5701 attgtccacc ctacacacca actccacctg cgaaacgctg gtacgtttac agcaaatggg
     5761 agtcgcccgc tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg
     5821 caaactttgc ccacattgtc gccgacagca aggggagcca atccatattc cagacaatgt
     5881 atggccgtcg ccgctgcccc gctggcaagc acccggttgt gtacattgct accacggttt
     5941 ttatggtcgc acggccttat ttgaagttct gcccataaca ccggtcatac gtcagcttat
     6001 ttccgctaat accgacgttg aatcgctgga aacgcacgcc cgacaggcgg gtatgcgaac
     6061 gctttttgaa aacggctgtc tggccgtgga gcaaggctta accacctttg aagagttaat
     6121 ccgcgtattg gggatgccgc atggcgagta agcaactctg gcgctggcgt ggcataaccg
     6181 gcgacggcaa tgcgcaagat gggatgctat gggcagagag ccgtgctttg ctgcttatgg
     6241 cactacagca acagatggtt accccactaa gcctgaagcg aatcgccatc aattctgcgc
     6301 agtggcgagg agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg
     6361 ggttaacgct ttctgaaggg ctggcactgc tggcggaaca gcatcccagt aagcaatggc
     6421 aagcgttgct gcaatcgctg gcgcacgatc ttgaacaggg cgttgccttt tccaatgcct
     6481 tatttccctg gtcagaggta tttccgccgc tctatcaggc gatgatccgc acgggtgaac
     6541 tgaccggtaa gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc
     6601 agttgaccga caaagtgaaa tcagcgttac gttatcccat tatcatttta gcgatggcaa
     6661 tcatggtggt tgtggcaatg ctgcattttg tgctgccgga gtttgctgct atttataaga
     6721 ccttcaacac cccactaccg gtactaacgc aggggatcat gacgctggca gactttagtg
     6781 gcgaatgggg ctggctgctg gtgttgttcg gatttctgct ggcgatagcc aataagttgc
     6841 tgatgcgccg cccgacctgg cttatcgcga ggcagaaatt gctgttacgc atcccgatta
     6901 tgggttcact gatgcgggga caaaaactca cgcagatctt tacgattctg gcgctgactc
     6961 aaagtgcagg cattactttt ttacagggcg tagagagcgt cagagaaaca atgcgctgcc
     7021 cgtactgggt gcaacttctg acacaaatcc agcacgatat cagtaacggt caacccatct
     7081 ggctggcgct aaaaaatgcc ggtgagttta gcccactctg tttgcaatta gtgagaacag
     7141 aagaggcatc cggctcgctg gatctcatgt tagacaacct cgcccatcat catcgggata
     7201 acacaatggc gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa
     7261 cgggaggaat tatcggtacg ctggtggtgg caatgtatct gccaattttc catttaggcg
     7321 atgcgatgag tggaatggga taacgctggc gtgttgctcc acgccagcgt tgggagatta
     7381 caggttgttg aagatgcggt tttcttgttc cagcacacgg ataaatgtgg tgcgcttggt
     7441 cagctctttc aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat
     7501 atcacgcgcg gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc
     7561 tgcgcgatat tccgcaacgc caccaacgtg acgtttcatc gcagactcgg agctcatgcc
     7621 gtagaacagc ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg
     7681 gcccgccagc atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg ctttcgcgac
     7741 atcgcccggc gtggtgcagc caccgtcgct gacgatcatt ccgcccagac cgtgcgcagc
     7801 atcggcacat tcgatcaccg cagaaagttg cggataaccg acgcctgttt tgacgcgagt
     7861 tgtacaaaca gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag
     7921 ctcctcacac atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc
     7981 ttcacgcgct ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacgtcaat
     8041 acaaacgaag tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc
     8101 atcagacgtg ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat
     8161 aaacgcttgc cactcttcga aagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga
     8221 agccagcgca gaggccatag aaaatgtgcc tacggtgtcc atatttgcgg cgataatcgg
     8281 cacgccggac cagctctgac ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc
     8341 ggaacggctt ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt
     8401 cagatcttct tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc
     8461 gcaactccag tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc
     8521 tttttttacg ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa
     8581 tttcgctttc gtgcgctcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat
     8641 aaattatgag gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg
     8701 ccaatgcgtt tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg
     8761 tggttgaacc aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga
     8821 ttgctgctga tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg
     8881 aagagaaaaa ctggcttaac gccctgctgc atccgctgat tcagcaagag acgcaacacc
     8941 agatccagca agcaacctcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact
     9001 cactgtataa aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta
     9061 agcgcaccat gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc
     9121 aggcaccgcg cgaagctcgc cttgccgtgg cagatgacgt cattgataat aacggcgcac
     9181 cggatgctat cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc
     9241 agtttgtctc acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa
     9301 tgaaaaaatg cgtacatggc tgcgcattga gtttttaatt caacaactca ccgtaaattt
     9361 gcccgtcgtt gactacgctg gcgctctgca tttcttccgc aatgtcagtg aattgctgga
     9421 tgttttcgag cgcggcgaag tccgcactga gttgttgaaa gaacttgacc ggcagcaacg
     9481 taaactccag acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat
     9541 tcagcagtta aaagcggcgg ggagcgtatt aatttccgcg ccccgtatcg ggcaatttct
     9601 gcgtgaagat cgtttgattg ccctggtgcg tcagcgactg agcatccccg gcggctgttg
     9661 tagctttgat ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag
     9721 ccaggtagaa acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct
     9781 ggatttaatt cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca
     9841 ggataacggt ggcgatgccg acttgctgcg cctgaatctg tcactcgatt cacagcttta
     9901 tccgcaaatt tccggtcata agagccgttt tgccattcgt tttatgccgc tggacagtga
     9961 aaacggacag gtaccggaac gtctggattt cgaactggcc tgttgctaag gagtaaaaat
    10021 gtcagaaact attacggtga attgcccaac ctgcgggaaa acggtggtgt ggggtgaaat
    10081 cagcccgttt cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc
    10141 tgctgaagaa aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga
    10201 agagccaaag cagtgacatt ttatctgatg cgacgctggc ctgataagac gcgggcagcg
    10261 tcgcatcagg catcgtgtgc aaatgtcgga tgcggcggga acgccttatc cgacctacaa
    10321 gaccttacat atcaacatgt taaaattgta gatactgata agacgcgcca gcgtcgcatc
    10381 gggcatcttg tgcaaatgtt ggatgcggca taaacgccta tccgacatac acacgcttaa
    10441 gcttcgcaag taccggttca ttggctggcg gaaaatcatc ggcattcaga ccgaccagcg
    10501 acatccactc accgggttgc ccttctttac cccacggctc cccttcccag ctttcgacca
    10561 gccagaacca cagtgttata tgcctgtccg ggaattcata ttccagtatt tcaaatagcg
    10621 aaagatgttg tggggtaatc ccgacttctt cctgaagttc acgcaccacc gcctgttccg
    10681 gcgtttcacc catttcaatt ttaccgccgg gaaactccag tttattcgcc atgtgcgcat
    10741 ctgctgcgcg acgggttata aagatttcat tgttctcgtt gcgaataata cctaccgcaa
    10801 tttgcagctt tttcattgtc ttaaacctat aaaaaaggcg cagaatcctg cgccttttac
    10861 ttcaactgtt agcttttatt gcaggcggcc atggcactgc ttatattttt taccagaacc
    10921 acacgggcaa ggatcgttac gtcctacttt gcgttcaccg gtttgcgccg ccagtgcagc
    10981 tgcggcagca gagtcgtcat cctgatggct aagctgctgc atttgcgcta aacgctcggc
    11041 ttccatacga cgctgttgtt ccagctcctc aacctcttca ggcatacgta cctgaacttt
    11101 gctcagcgtg ctgataactt catattttaa cgactccagc atcgctgcaa acatggagaa
    11161 cgattcacgt ttgtattcct gcttcggatc tttctgtgca tagccacgca ggtggatacc
    11221 ctgacgcaga tagtccatcg cagccaggtg ctctttccac agagagtcaa gcgtttgcag
    11281 catgacgcct ttctcgaagt gacgcatcat ctcagcacca accacttctt ctttacgctg
    11341 atacacttcg atggactgcg ccagaatgcg ctcacgcagc gtctcttcat gcagttctgg
    11401 ttctttatcc agccactcgg caattggcaa atcgaggtcg aaatcgttct tcagacgttc
    11461 ctgcagcccc ggaatatccc acatttcttc cagcgactgt ggcggaatgt aggcatcaat
    11521 ggtcgctttg aacacatctt cacgaatgct gttgatggtt tcgctcacat cgctgacatc
    11581 cagcagttcg ttacgctggg agtaaatggc gcgacgctga tcgttagcca cgtcatcata
    11641 ttccagcagt tgcttacgaa tgtcgaagtt acggctttca actttacgct gggcgttggc
    11701 aatcgctttg gtcacccacg ggtgctcaat ggcttcgcct ggcttcatac ccagtttacg
    11761 catcatgccg gatactcggt cggaagcaaa aatacgcatc agcgcatctt ccatcgacag
    11821 gtagaaacgg gaagaaccag catccccctg acgaccagaa cgaccgcgca gctggttatc
    11881 gatacgacgg gattcgtgac gttcagtacc gatgatatgc aggccacctg ctgccagtac
    11941 cgcatcgtga cgtacctgcc agtcggcttt aattttttca atttgctctg cagtcggatt
    12001 ttccagcgcg gcaacttctg cctgccagct accaccgagc acaatatcgg taccacgacc
    12061 cgccatgttg gtcgcgatag tcaccgcagc cgggtaacct gcctgagcaa caatcgccgc
    12121 ttcgttggca tggaatttgg cgttcaggac gttgtgctta ataccggctt tggtcagttc
    12181 atttgacacc agctccgatt tttcgatgga gattgtaccc accagcaccg gctggccttt
    12241 cgcagtgcgt tctttgatat cttcaatgat cgcctgaatt ttttccgctt cagtcatgta
    12301 gaccaggtcc ggcagatctt tacgaatcat tggacggttg gtcggaacaa cgacagtatc
    12361 cagcttatag atggagctga attcgaaagc ttcggtatca gcagtaccag tcatccccgc
    12421 cagtttttca tacagacgga agtagttctg gaaggtgatc gaagccagcg tttggttttc
    12481 gttctggatc tgcacacctt ctttcgcttc cacagcctgg tgcagaccat cggaccagcg
    12541 acggccctgc atggtacgac cggtgtgttc gtcaacgatg ataacttcac catctttaac
    12601 gatgtagtcg acgtcgcggg taaacagcgc atgagcgcgc agcgccgccg ttacgtggtg
    12661 catcagcatg atgttggccg gagagtacag agactcacct tcatccatga tgccttcttt
    12721 aaccagcagt tcttcaatca gaaccagacc acgttcggtc aggttcacct ggcgagattt
    12781 ttcatccacc gagaagtggc cttcgccctg gaaggtttcg gagtcttctt tttcctgacg
    12841 gatcaggtgt ggaataattt tattcacgcg tttatacatt tccgagctgt cttctgccgg
    12901 gccggaaatg atcagcggtg tacgcgcttc atcgatcagg atggagtcca cttcgtccac
    12961 cagcgcatag tgcagtttac gctgtacacg ttcttcagga ctgaacgcca tgttgtcgcg
    13021 caggtagtca aagccgtatt cgttgttcgt accgtaagta atgtcagctg cgtaagcttc
    13081 acgctttgcc ggtgctggca tgcccggcag gttgatacca actgtcaggc caagaaattc
    13141 aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg tagtcgttga cggtaactac
    13201 gtgcacgcct ttaccggtta gtgcgttcag gtaagcaggc agcgttgcgg tcagggtttt
    13261 accttcaccg gtacgcattt cggcgatgca gcgttcgtta agaaccatac cgccgagtaa
    13321 ctgaacgtcg aagtgacgca taccaaagac gcgcttactt gcctcacgca ccacggcgaa
    13381 agcttccggg atcagatttt ccagcacttc gcctttttcc agacgtgcac gaaactctgc
    13441 ggttttccct ttcagttctt cgtcggagag tttttccatc tccggttcca tggcattgat
    13501 gatgttgacc actttgcgca tccggcgcag ggtgcgatcg ttacgactac cgaaaacttt
    13561 agttaataat ttgattagca taataaaatc tcaaacgccc cgcgttgcgg agttaataaa
    13621 atgaagtaac ggtttattgt tgttagctga ggcgttgagg gccagcacgg atgccttgcg
    13681 cctggcttat ccagacgggc gtgctgaatt ttgcttgcgg ggtaaaatgc gcataatcaa
    13741 tgcgataacc cttttcagac ggcgtgcctt cctgggtcaa cagcgcgctg agcgtatcca
    13801 gtaatgcaag atgttgcgcc tgaagaggca aagattcttc agcaacgggc agcgtttgcg
    13861 gtgccattgc gaaggaaaga tgacggatta ccgtgcgaat ggcatgttga tgccagtaat
    13921 caacggaata gttcgaattc gggcgacgcg tgttcgcttc cagcaaggcc aattgaccaa
    13981 agttaacttt ggctgaaggc tcgtggttgc gggttgtcgc ttttgcgggc gcgtttggtt
    14041 cggcggcgtt gctgagcgca ggcaaaccta aactcgccgc gaccatccct aataagagat
    14101 gcggccagaa gtagcgttta ccaaactgtc gccagcgcgt cagtattcca ctcacgttat
    14161 tgccatcccg atcgtattca aacgtattta gcgcacaaat cttatcatta aagcctgaag
    14221 tccacagcac gaatgaagat aagttgtgcg taaaagtgct taagaaaaca gcgttcgcac
    14281 cgttttttat cgttcttata aaattcaaaa aaaaacgact ggttcacctg gccggagaga
    14341 gtgccagatt tgccagtcga attttatacg acagtataaa tgtcgttatg ccagtacggc
    14401 tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc tggaaggtca catattccca
    14461 ggcttcctgt ttcgccagga cagcctgcag cagtttgtta ttcagtgcat gaccagattt
    14521 ataagcggta aatgcaccaa taatattgtg accacacatg aacaagtcac cgatcgcatc
    14581 gagcattttg tgacgcacaa attcgtcttc aaaacgcagg ccgtcttcgt tcagtacgcg
    14641 ataatcgtca acaacgatgg cacaatcgaa gctgccgccc aggcacaaac cacgggactg
    14701 cagatattcg atatcacgca tgaaaccgaa cgtacgcgca cggctgatct ggcgcataaa
    14761 cgcatcagcg gagaagttca tcgcatagcg ctggttgctg gaatcaatag ccggatggtt
    14821 aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc ttaaattcag cccacttatc
    14881 gccatcttcg acacgaacag tctctttgat gcgaacaaat ttcttggcgc agttcagctc
    14941 gtcgatacca gcgtcaagca gcaggtatac aaacggagcg gcgctgccgt ccatgatcgg
    15001 gatttccggc gcgttaactt cgataacaat gttatcgatg cccaggcccg cgagagcagc
    15061 attgaggtgc tctacggttg aaatccgtac atcatgctcg ttgaccagac acgtacagag
    15121 catggtatca cgcacagatt tggcatcggc cgggaaatct accggtggat tcaagtcggt
    15181 gcgacgatag atgaccccgg tgttggccgg cgcagggcgt aacgtcaggg tgactttctt
    15241 gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt ttaagtgtcc tttgtttgat
    15301 catcgtatta tctcgccaaa ttacctatcc aaccgaagtg tactatacat tcggcgggcc
    15361 agtttagcac aaagagcctc gaaacccaaa ttccagtcaa ttcttaatca gcttgcttac
    15421 gcaggaatgc tgggatatcc agataatccg gctctttcgc agtttgcggc gcattgtcat
    15481 tcacgacttt agcaaccggc ttctgctcct gggtcagcgg agccatcccg tgctgctggt
    15541 agcgatccat cactggctgc tgaacctgct tattggtcac cagagtgatt tcaggacgtt
    15601 tgtccatgcc gatacctgtc gcaacaacag ttacgcgcag ctcgtcattc atatccgggt
    15661 caagagaagt accgataacc acagtcgcgt tgtcggaagc aaatgcacgg atggtgttac
    15721 ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc tgtgatgttt accagtacgc
    15781 cgcgtgcgcc agacaggtcg atatcttcca gcagcggaga agagatagcc atttcagcag
    15841 cttcttccgc acgatcttca ccgctcgcca caccagaacc catcattgcg tagcccattt
    15901 cagacattac ggtgcgtacg tctgcaaagt ccacgttcat caaacccgga cgagtaatca
    15961 gttcggcgat accttgcaca gcgcctttga gtacgtcgtt cgctgcgcca aacgcatcca
    16021 gcagggagat accgcggccc agaactttca gcagtttgtc gttcgggata gtgatcagag
    16081 agtccacatg cttggacagt tcagtgatcc cctgctccgc gaatgccata cgcttcttgc
    16141 cttcaaagtt gaagggctta gtgacgacag caacggtcag gatacccaaa tcttttgcca
    16201 cttcagcgac gactggagct gcgcctgtac cggtaccacc acccataccc gcagcaataa
    16261 agaccatgtc tgcaccttcc agcgccgcac gcagtgcatc gcgatcctca tcagccgcat
    16321 tgcggccaac ttctggatta gcgccagctc ccagtccttt ggtgataccg ctaccgattt
    16381 gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc atcggtattt accgcgaaga
    16441 attcaacacc ttcaatgcgc tcgcgcacca tgtgttcaac agcattaccg ccgccgccgc
    16501 cgacgccgat gactttaatc accgcgtcat tggtaagttc cattggttca aacatagttt
    16561 ctctccgatt tgtgcctgtc gcctgagacc gtaatcattg tcggcctcat aaaaattaaa
    16621 actcttttcg cagccaacta ttgagtcgct tgatccacga gccaactgat gctgtaacac
    16681 gtttttctac ttcagcttca ccgttaagat gtgactcttt cccgtaatga agcaatccca
    16741 ccgccgtcga ataatacggc tcctgagcat aatccgttaa accggtaatg ttcagcggcg
    16801 cgccgatacg cacctgcgta tgaaacacgc gctgagcaca ggctgcaaga ccttcaatct
    16861 gcgctgcgcc accggttaat acaatgcctg ccgccaggtg atgtttaacc ccttgttggc
    16921 gaagcttttc ctgcaactgc aatatctctt cgttgaccag gttgagcagc tcggtatagc
    16981 gcggctcgat cacctctgcc agggtctgac gttgcagact ccgcggcgga cgaccgccta
    17041 cgctcggcac ttccacgctc tcatcttttc caacgatgga acccagcgca caaccgtggc
    17101 gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa ggcgtaagcg atatcactgg
    17161 tcacgacatt gccagcataa ggaattacct tagtgtggcg caatgcccca ccggtataaa
    17221 cggcgatatc cattgtacca ccaccgatat cgacgacgca gacacccagt tcacgttcat
    17281 cttccgtcaa taccgaataa cttgatgcca gtccggcaaa tatcagttgg tcaactttca
    17341 gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat atcgttgtga catgtgatca
    17401 ggtgcacttt tgcctgcatc cgcacgcccg aaagtcctac cggattcttg atcccttcct
    17461 gatagtcaat cgcatactct tgcgggatca catgcagcac acggtgttca tcgcgcacac
    17521 gcaccgattt cgcggtatgg acaacgtttt ccacatcttc ttgcgtcact tcttcttcag
    17581 aaataggcac cataccaatt tcattctggc agctgatgtg cttaccagaa agcgccagat
    17641 ataccgaaga gatctgacaa tctgccatca attctgcctg gtcaatggcg cgctgtacgc
    17701 acttgaccac ggattcgagg tcgttcaccc cgcctttatc cataccacgc gacgggcagc
    17761 tgcccacgcc aatgatattg accataccgt cgggcagaac ttcccctact aaagcggcaa
    17821 ccttcgcggt accaatctcc agtcctacta ccagttttct gtccgtcgcc ttaatcattg
    17881 ttgttctgcc tgtgcctgat tttgttgctg agtagattcc tctggcggca agggcgccca
    17941 gcctactgcc gctccagagt cataacgcaa atcaacgtag ctaatccgtt tgccatcggt
    18001 ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag cgagccaaac gtttcatcgt
    18061 atcgccccgg ccaagattga gcttaatatc gttattcagc gtcaactgcc aggaacgccg
    18121 cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt gccagcatct gccccatttc
    18181 gcgatagccc tgcaacactt cattggcgct gccttccggg ccatacagca ttggaagcac
    18241 ctgcttgctg gtgcgatctg gcggcacgct gaaagtgttt ccttccgcgt ctaccatatg
    18301 ttgatcattc caccgcgcaa tcggcacata ttcaaccaga tgaatcttca attcatcagg
    18361 ccactgcttt ctgacgctca cctgcttaat ccacggcagg cgttgttcta tttgcgtctg
    18421 gatgatgttg acatcctggg tcataaaggt acctggctca cccaatgcca ggatcgactg
    18481 ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc aacaccagct ttgagagcgg
    18541 caggcgttgc gcatcttcca tccagcccaa cacgacccag ccgctcacca acactgtcgt
    18601 taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt ccattattgc ggcgagaaga
    18661 aacctcttct tcgctgtttc gcgtgttcag agcagcctgc gacatattag tccgccagtt
    18721 ccagaattcg tactaccaac tgcgagaagc tcatacctgc ctgacgtgcc gccatcggca
    18781 ccaggctgtg gctggtcata cccggtgagg tattggcttc cagcagataa aactgtccat
    18841 cgctgtccag cataacgtca atacgtcccc atcctttgca acctaacgtc gtccatgctt
    18901 tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttc cagacctgcg gggcagaaat
    18961 actgtgtctc atcagagaga tacttcgcct cataatcata gaaggttccg gacggttgaa
    19021 tacgtattga cggtaaaatt tcttcaccga gtatcgcaac cgtgaactcc ggcccactta
    19081 gccatttttc aatcaatact tcttcatcgt gctgaaatgc caatcttaat gcatcttgta
    19141 gagcattttc tgctactact tttgacattc ccacactgga accttcgcgg ctcggcttaa
    19201 cgataaccgg caaacccaga gcagaaattt ctgctaactg cttatcactc aggccttttt
    19261 caaactctgc gcgggttaac gctacccacg gagcgaccgg taaaccggca ccttgccaca
    19321 gaagtttgct gcgtagttta tccattgaaa gcgcagatgc catcactccg ctgccggtat
    19381 aaggcaagcc catcagctcg agcatgccct gcagcgtgcc atcttcaccg ccgcgaccgt
    19441 gtagcgcgat aaacactttc tgaaagccca tcgacttcag ttgcgtcacg tcgacttctt
    19501 tcgggtcgac aggattcgcg tcaataccgc cttcacgcag cccggctaac accgctgcgc
    19561 cagaattcag agaaacttcc cgctcagcgg aggtcccacc caacaggacc gcgattttat
    19621 cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt tgatttcagc taaagaacgg
    19681 gcaatttttc cgatattacc agccccctga acgagaatca ggtcgttacc ggttaatacc
    19741 ggagccagca tctcggctac ctgcgccggg tccggcacca gaatgggatc aattttccca
    19801 cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc ccggaattgg cgcttcgcca
    19861 gccggataca cttccagcat caacagggta tcaacctgcg tcagcacatt ggcgaaatca
    19921 tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa acagcattac caggttttta
    19981 tccggccagc ctgcacgcgc cgctttaatg gtggcgtcca cttccgtcgg gtggtggccg
    20041 tagtcatcga ccagcattgc cgtaccgctt ttaccattca caggctccag cgggaattca
    20101 ccgaggaaat caaaacggcg accagtcccc tggaagctct ccagcgcacg caaaatagcc
    20161 tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag ctgcggcgtt cagcgcgtta
    20221 tgacggcctg gcgcattcag ggtgacgcgc atcggctctt tgtcctggcg cagcagcgta
    20281 aagtgcccct gcgggccaat ctgctgataa tcttctacac gcacgtcggc atcttcgctg
    20341 aagccgtaag tcgtggtctg acgtcccaca cgcggtaata attcgcggat caccggatca
    20401 tcaacacaca tcaccgcacg accgtaaaac ggcaggttgt gcagaaaatt aataaaggtc
    20461 tgttttaaat tctcaaagtc gccctggtag gtatccatgt ggtcggcttc gatattggtg
    20521 acaatcgcca ccatcggttg cagatgcagg aacgatgcat cactctcatc tgcttcggca
    20581 atcaggtacc gaccatgccc caaacgcgca tgaacgcccg ccgcttttac cagcccaccg
    20641 ttaacgaagg ttgggtcgag tcccgcttct gcgtagatgc tggaaaccat cgcagtggtt
    20701 gtcgttttgc cgtgcgttcc ggcaatggcg atgccatgac gaaaacgcat taactcagcc
    20761 agcatttcgg cacgacggat caccggaata cgcgcttcat gagctgcgac aatttccggg
    20821 ttatcggcag aaatcgcgct ggaaacaacg accacgctgg catcacgtac gttttccggg
    20881 cgatggttga aataaatcgt cgccccaaga ttcattaact gctgcgtgac cggatttggc
    20941 gctaaatcgg aaccactgat ctgataacct tcattggcca gaacttcggc aataccgccc
    21001 ataccggcac caccgatgcc gacaaaatgt atgtgccgaa cgcgacgcat ttcgggcacg
    21061 atggaacgca gttttgccaa ttgttgtgta ttcattcttt acgccattaa cttcttaaat
    21121 tcatacgatg caaaaggcat cgctacaatt acgcccgggc agcccggctc acttcatttg
    21181 ccactcgctc ggtggcatcc ggaatggatg cggcgcgggc gcgttctgcc atggttaata
    21241 aggtttctcg cgaccacccg gccagggtgt tggcgacagc atccacgcta agctgtggct
    21301 gctcgataat tttggctgcg cccgcttttt ccagcggtag cgcattccag tattgctggc
    21361 ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc tgccgcggcg atttcactca
    21421 ccgttaacgc accggagcgg caaacgacga catccgccca cgcatacgcc gccgccatat
    21481 catcaataaa ttccgtcact ttatgctgcg gttgccccgc ttcggcatac gcctgttcaa
    21541 cggattgttg cgaaccttta ccgctctgat gccagatagt gactgaatca cccagtttcg
    21601 cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc ctgggaacca cccactacca
    21661 acacacgaac cggaccttca cgtccagcca aacgttgctg cggcaacggc agcgccagca
    21721 catcggtacg caccgggtta cccaccactt ccgcattagg gaaagcgcct ggaaacgcct
    21781 gcatcacttt ggtggcaatc ttcgccagcc atttattggt taatcccgca ataccgtttt
    21841 gttcatgaag cacaaccgga atacctaacg accacgcggc cagaccacct ggacctgata
    21901 cgtagccgcc cataccgagc accacgtcag gtttgtacgc tttcataatc gcccgcgcct
    21961 gacgccaggc gttgaagata cgcagcgggg cagctatcag tgcttttata ccttttccac
    22021 gcagaccaga gatacgaatg aaatcaattt cgatgccatg ttttggcact aagtccgctt
    22081 ccatacggtc ggcagtcccc agccagcgaa cttgccaacc ctgagccatt agatggtgcg
    22141 caaccgccag tcccgggaat acatgtccac cggttccgcc tgccatcacc attaatcgct
    22201 tcccttgacc actcatcgtg aacctcgtac aaacgcctgc gctttctcca gacgcgtttc
    22261 ataatcaata cgcaacagca tcatgatggc tgtcgacata atcagtaagc tcgaaccacc
    22321 gtaactgatc agcggcaatg tcagaccttt ggtcggtaac atccccgccg ccgcgcctac
    22381 gttaaccagc gcctggaaac taaaccagat accaatagaa caggcgagaa aaccggaaaa
    22441 acggtggtca atttctaatg ctttgcggcc aatcgacatc gcgcgaaaag cgacgaagaa
    22501 taccattaaa agtgccagca ccacaccgac ataccccagt tcttcgccga taatggcgaa
    22561 aataaagtca gtgtgcgctt ccggcagata ctccagtttt tgtaccgagt tacctaaacc
    22621 ttgtccccac agttcaccgc gaccaaacgc catcaacgat tgcgttaact gatagccgct
    22681 gccaaaggga tcttcccacg gattccagaa tgcggtaaca cggcggatac ggtacggttc
    22741 ggcgaggatc agcaacacaa ccgctgaaat gcccataccg ataatggcaa tgaactgcca
    22801 caatttcgct cccgccagga acaacatcgc cagcgtagtc acaaacaaca ccaccaccgt
    22861 accaaggtct ggctgtgcca gcagtaacac tgccaacacc agaattacgc ccatcggttt
    22921 caggaagccg cgcaggttat tacgtacttc gtcgccttta cgcaccagat agttggcgat
    22981 atagcaaaac agcgacagtt ttgtcagctc cgcaggctgg atacgcagca aaccgagatc
    23041 gatccaacgc gatgcccctt taaccgagct acccactacc agaacgatca tcagcaggat
    23101 gatagatccg agcagcatcg tggcactgta gcgttgccag aactccatcg gcagacgcag
    23161 cgtaatgatc gccagaatga atgccaggat cagatagacg ccatcacgct tcgcgaagaa
    23221 gaacggatcg ttggttaagc gttgccctat gggcattgac gccgaggtca ccatgataaa
    23281 gccaatcgcc gcgaggccga aggtcagcca cagtaaggtg cgatcgtaca tgatcaggct
    23341 gtcggtatct ttttcccgcg agcccatcac ccagcccttt agcgccgtgg agatccagac
    23401 cagaatactg aatcctggca ggcgcggcat tttcaggcga gggagagata aacgcatcaa
    23461 cctaactcct tcgccagacg ggcaaactca ttgcctcgtt gttcaaagtt cttgaattga
    23521 tcaaggctgg cacaggccgg ggagagcaga accatatcgc ccggctgaac acgcggagcc
    23581 agcaagcgca tcgcctgttc catggtttcg gtttgttctg ccacttccgg gcgtagcgcc
    23641 gccagctgcg cgccgtcccg accgaaacaa tacagacgta cattatcgcc attcaggtaa
    23701 cgcgccagtg ggctaaagtc cgccgattta ccatcgccac ccagcaacaa atgcagcgtg
    23761 ccgtctacat gcaggccatt cagcgccgct tccgtactgc cgacgttggt cgcttttgaa
    23821 tcgttaaccc agcgcacacc gttatgctcc agcacaactt caaagcgatg cggcagacca
    23881 gtgaatgtgg ttaacgcttt caggctgctg gcacgcggta accctgcggc atctgccagc
    23941 gccagcgccg ccagcgcatt ggtgtagtta tgctgcccgg aaagtttcat ttctttcacg
    24001 ttcagcactt tctcgccttt cacccgcagc caggtttcac cctgctgatg attcaggtga
    24061 tagtcgccca tgttgacgcc aaagctgacg cagcgttcat ccgcaccgcg aatcggcatt
    24121 gttaaggcat catcagcatt aaccacgcaa atttttgcgt tttcgtaaat gcgcagtttt
    24181 gctgcacgat actgttgtaa accaaacgga tagcgatcca tatgatcttc agtcacgttc
    24241 agaatggtcg ctgctaccgc ctgtaaactg gaggtggttt caagctggaa gctcgacagc
    24301 tccagcacgt acagttcgca ctcatcatcc agcagcatca acgcaggcag gccaatattg
    24361 ccacccacac caacgttaac ccccgccgct ttcgccattt cacccactag cgtggtgacc
    24421 gtgcttttgc cgttagaacc ggtgaccgcc acaatcggtg cttgtgcttc acgacagaac
    24481 agctcgatat cgccaacgat ttcgattccg gcatcagcgg cagcgcttaa ggatggatgc
    24541 gccagtgcaa taccgggact ggcgacaatc agatcggccg ccatcagcca ttcatcattc
    24601 agaccgcccg tgtggcgttc tacagcttcg ggtaatttat ccaggccagg cggtgtcata
    24661 cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga aaaagtccac gcaagaaagc
    24721 ccggtgaggc ccaggccgat aatgacgaca tttttaccct ggtaatcagc catgattaac
    24781 gtaccttcag cgttgccaga ccaatcagga ccagcatcag cgaaataatc cagaaacgta
    24841 caatgacgcg cggttccggc cagcctttca gttcatagtg gtgatgaatc ggtgccatgc
    24901 ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg caggatgacg gaaagtgttt
    24961 ctaccacgaa cacgcccccc ataatcacca gcaggaattc ctgacgcagc agtacggcga
    25021 taatacctaa cgcaccacct aacgccagcg aaccgacatc gcccataaag acctgcgccg
    25081 gataggtgtt aaaccacagg aagcccagcc ctgccccgac tatcgcggta cagacaatga
    25141 ccagttcccc ggcgtgtcgt agatacggta tgtgtaaata gctggcaaag ttcatgttgc
    25201 cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc ataattgcca
    25261 gaccgtcgag accatcagtc aggtttaccg cgttgcctgt acccacaatg acgaagtaag
    25321 ccagcagaat gtagaacagc cccagttgcg gcatcacatc tttaaaaaat gggaccacca
    25381 gctgcgttgc gggcgtgtct ttgccaacaa ggtacagcgc gaaggcgaca cccagcgcaa
    25441 tgaccgacat ccagaaatac ttccaacgag cgatcaaccc tttggtgtct ttacgcacta
    25501 ctttgcgata atcatcaaca aagccaataa caccgtaacc taccagcact accaacacgc
    25561 accagacgta cggattggac gggtaagccc acagcagtac ggagatcaca atcgctgtca
    25621 ggatcataat cccgcccatg gtcggcgtac cgcgcttgct gaagtgtgat tcaggaccgt
    25681 cgttacgcac cacctgacca aaggaaagtt tttgcaaatg agcaatcatg cgcgggccca
    25741 tccacaatga gatgaacagc gcagtcagca ggctgacgat ggcgcgaaac gtcagatagg
    25801 aaaagacgtt aaagccggaa taatatttga ccaaatgttc ggccagccaa actaacatgt
    25861 cccattctcc tgtaaagcgc gtactacctc ttccatggca gcactacgtg aacccttaac
    25921 taaaatcgta attacctgtt gctcagcaat cagtgattta agacgcgcga taagcgcagt
    25981 tttatcggaa aaatgttcgc caacaccgct ggcagtgctg atagcatggc tttgtttgcc
    26041 cacgcttaac acgcggtcaa taccagctgc ttttgccgct tcgcccacct gtatatggca
    26101 ggcttcgctt tcagcgccca gttccgccat atcgcccacc accagcacgc ggtagcccgg
    26161 catttcagcc agtacctgga ctgctgcggt cattgaaccg acattggcgt tgtaggagtc
    26221 gtcgagcagc aactggtttt ccgccagttg gatggggaac agacggcctg gaacagcttt
    26281 cagatttgcc agccccgctt tgatagcatc aagcgttgcg cccacggaca tggagagcgc
    26341 agcggctgcc agcgcattcg caatattgtg acgccccggc aacggcagca gaacatccac
    26401 gctaccggtt ggggtttgca gggtaaattc cgtaccgtgc gaagtcacat ggatgttggt
    26461 ggcggtgaaa tcgctgttgg cggcattagg tgagaaacgc cacactttgc gtgagccaat
    26521 tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc ataatggcga taccgttttc
    26581 cggcaggccg ctaaagattt cacctttcgc tttcgcgaca cccgcaagcg agccaaaacc
    26641 ttccagatgc gccgctgcca ggttgttgac cagcgccgct tccgggcgag tcagactcac
    26701 agtccaggca atttcgccct gatggttcgc gccaagttca attactgcgt aatcgtactc
    26761 tggcgttaag cgcaacagcg tcatcggtac accgatgtcg ttgttgagat tgcctgccgt
    26821 ataaagcgtg ttgccacact ggcttaaaat cgctgccgtc atctctttaa cggaggtttt
    26881 gccggaggaa ccggtcagag caaccacgcg cgccggaact tgctggcgaa cccatgcagc
    26941 cagttcacca aacgccagac gagtatcctt gacgattacc tgcggcagat cgatatccag
    27001 cggacggtta accagtagtg cacccgcacc gccagctttc gcctggtcag caaaatcatg
    27061 ggcatcgaaa cgttcgcctt tcagggcaac aaacaggcaa cccggcgtca gttttcgtgt
    27121 gtctgtggtt acagcatcaa gggtgatatc tgcccctttt agttcaccgt tgagaatatc
    27181 ggtaagttgg ctaagggtta cgctaatcat gcaatcaccc ccagcagacg cgccaccgtg
    27241 acgcgatcgg agtagtccag acgctgattg ccaacaatct ggtaatcctc atgacctttg
    27301 cccgcgacca gtaccacatc attctcttta gcctgcataa cggcgcaagt caccgcttca
    27361 gcacggcctt ccatcacttt ggcatgtccg gcatctaaca ttcccgccag aatatcgttg
    27421 atgatggcac gcggttcttc ggtacgcggg ttatcgtccg tcaccaccgc cacgtcagcg
    27481 aactcttcag caattgcgcc catcagtgga cgtttgcctt tatcacgatc gccaccacag
    27541 ccaaagacgc accacagctt gcccgcacag tgcaggcgcg ccgcctgtaa ggctttttcc
    27601 agtgcatccg gtgtatgcgc gtaatccacc actaccgtcg gtttgcctgg cgcagtgaac
    27661 acttccatac gtccacaaac cggctgcagg cgtgcggcag ttttcagcag gtcagccaac
    27721 ggatagccca gcgccagcag cgtcgccagc gccagcagca agttgctgac gttaaaagcg
    27781 cccatcagat ggctttcaat ttcgccatcg ccccagcttg agctaaagcg aatcgtcgca
    27841 ccgctgtcgt gatagttcac ttctgtcgct ttcaaccagc gtccgtgaca gttcggattg
    27901 atatgatctt ccattgatac cgcaaccgcg tccggcagtt ttgccagcca gcggcggccc
    27961 acttcatcgt cggcgttaat aatcgcctga ccgcaatgat gctcagaata aagcagccat
    28021 ttcgcggctt cgtagtgttc catatcacca tgataatcaa ggtgatcgcg gcttaagttg
    28081 gtaaagaccg acgccgcaaa tttcaatgcc gccacacggt gctgtaccag tccgtgggag
    28141 gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca gccccgccag ctcatgctga
    28201 acatcgactg ccgaaccggt tgtattttct gtcgggatca ctttccccag caggccgtta
    28261 ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt ggctccactg cgccagcagc
    28321 tgggtcgtcg tggttttgcc gttggtgccc gttacgccca cgagacgtaa attgtcagag
    28381 ggttcatggt aaaagcggcc cgccagtgca gataaacgct cgttgagctg gctgagatag
    28441 atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg tcgcctcatc tttcgcctct
    28501 gcaataatgg cagccacacc ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc
    28561 tgatgaccta ctacagctac aaagagatcg cccgccgcag ccacacggct gtcgagtgtc
    28621 atctctcgca gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa
    28681 ttacgatctg ccacctgtcc cctcgccttg attaatcaca aattcatttt tatcgcccgt
    28741 tgtcagcgca tccggttcga tgttcatggt acgcaatacg ccgcccatga tggcaccaaa
    28801 gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt tgataacaac
    28861 aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct gcggtataag caatatattt
    28921 attgatgtag cgaccgtccg gcccgacctt tttcgcggta ccggttttaa tggcgatacg
    28981 atagccttta atcgccgcct tcacgccgcc gccgcccggt agcgccacgc tttccatcat
    29041 atgcaccacg gtgcgaacaa tggattccgg gaagactcgt tcaccgggaa ccggggggtc
    29101 aactttggta atcgacagtg ggcgataaat gccgtagctg ccgatagttg cgtagactcg
    29161 cgctaactgt aacggtgtta ccattagccc gtagccgaaa gagaaggtgg ccctctctat
    29221 gtcagaccac cgttgttttt gaggatataa gccactgcgt tctccgacca accccaaatt
    29281 ggtcgctttt cccagtccaa aacgtgagta agtatctact aacgctgagg acggcatcgc
    29341 taacgccagc ttggaaacac cgacgttact cgacttctgt aataccccgg tcagggttaa
    29401 ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta attcgataag gaacggtatt
    29461 cagtaccgag ttttcccgca ctacgccacg ttgcaacgcg gtcatcacca ccatcggttt
    29521 aaccgttgaa cccggttcaa acacgtcagt gatggtacgg ttacgcatcg cctctttcgg
    29581 cgtgccactc agattgtttg ggttgtatga cgggctgtta gccatcgcca gcacttcacc
    29641 ggtgttgaca tccaccagca cggcgctacc agattcagct ttgttaaagg ccaccgcgtt
    29701 gttcagttcg cgataaacca gcgcctgcag gcgttcatca atactcagcg ccaggttgtg
    29761 cgccgcctga ctgtcggtgg aagagatatc ttcaattacg cgaccatagc ggtctttacg
    29821 cacaatgcgc tcacccggct gcccggtaag ccatttatcg aaactcttct cgacgccctc
    29881 aatcccctga ctatcgacgt tagtaaagcc gatgaggtga gcggtcactt cgccggatgg
    29941 ataataacgg cgagattctt cacgcagatg aatccccggc agtttcagtt ttttgatgta
    30001 ctctgccatg tcagggttta cctgacgcgc cagataaata aagcgccctt tcgggttggc
    30061 gttaatgcgg gctgaaagct gatccagcgg aatattgagt gcgttagcca gcgccttcca
    30121 gcgatcaccg acgctgatac cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc
    30181 ttttaccggc acgctcaccg ctaacgggcg accagaacgg tcagtaatca tgccgcggga
    30241 ggtggaaact tgctgaacgc gaagagaacg catgtcgccc tcttttacca gcatatccgg
    30301 ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca
    30361 gccgcataac aacgcaaaac gccaactgat aaagttggca tgttcttcct gacgttttgg
    30421 tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt gcactacgat
    30481 attttcttgt gacggatcaa catgctgcat ttgcagcttt tccgtggcga tcctttccac
    30541 ccggctatgg tcgccgagcg cattctcttc aaggatcagg ttgcgccatt caatgtctaa
    30601 agcatctcgc tccagcacca gttgttcgcg ctgtgcggtc agtaaacggg tatggtgcgc
    30661 cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt
    30721 cccaaatcgc aaaagatcgt caccgataac accaggcaat gcatggcgct cgtggcttcc
    30781 catcgatcct ttaactttgc ttagagcttc tgtcactctg ctgatcatgc gttcgtcctc
    30841 tctgcaatac gcagaactga actacgggca cgagggttct cagccacctc ttcttcaccc
    30901 ggcattaact tgcctagtgc tcgcagctga cggccaccca gttttttgag ctgctcttca
    30961 gtcatcggta accctgccgg aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc
    31021 acaatacggt cttccagcga gtggaagctg atgatcgaaa gccgcccacc cggggccagc
    31081 acgttgagcg agctttttag cgcctgctct atctcctcca gttcactgtt tacccaaatg
    31141 cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact tatctttcac cggcgttgca
    31201 gcagccacga cttccgccag ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg
    31261 acaatggcgc gggcaatgcg tttggcaaaa cgctcttcac cataggtttt caatacccag
    31321 gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg ctgactgccc acgggttggg
    31381 tccatacgca tgtccagcgg accatcgcgc ataaaggaaa agccgcgttc agcatcatca
    31441 agttgcggtg aagagacgcc aagatcgagg agaatgccgt cgatcttgcc gataagatcg
    31501 cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga
    31561 tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc
    31621 ccctcttcgc caagctgcga gaggatcaga cgtgagtgac caccgcgacc aaaagtccca
    31681 tcaatgtaga tgccatcagg acggatattg aggccattaa cggcttcatc cagcagcacc
    31741 gtagtatgtt tatagttttc catcatttta tagagacaag tcctgcagtc gctccgataa
    31801 gtctccagta gccaactgct ctgcgtcgat atcttccttg acctgttgat gccaggttgt
    31861 ttcatcccac agctcaaact tgttgaactg tccaaccagc atcacttctt ttgtcagccc
    31921 ggcatgttgc cgcagtactg gcgcgattaa caatcgacct gcgccatcca tctgacattc
    31981 gctggcatga cctaacagta ggcgctgcac acggcgctca accgggttca tgctcgacag
    32041 acgcgataat ttttgctcga taatttccca ttcaggcagg gggtaaagca gcaggcacgg
    32101 gtgatgaatg tcaatggtgc aaaccatttg accggcagcg ttctcaagca gctgttcccg
    32161 ataacgggta ggcactgata agcgcccttt gctgtcgaga ttgactaacg ttgctccccg
    32221 gaacatgcca gcctcacccc ttattcccac tttgccccac aatttcccac tgaaaggagt
    32281 ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc
    32341 gatgtttacg gctaatatca gcgttgttta ttttacgtcc aacgtgcgaa gcaaattaac
    32401 gttatgaata gctgtaagaa aaaatccctc atcgtcatca ccgttttaaa atgactcact
    32461 attttccaca gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt aggacatatc
    32521 ttccccggtt aacagtccct gttgcgtctg tgtggcgctt gatgccagcg ggtttgcgca
    32581 aaagcaaaga aaagtgtttg ttaaatcggc aattcatctc tgcatgtaac aaaataatgc
    32641 aaaaacgcgc tatcgatatc acttaattat tgtgaatttc agctggtgag gtaattaacg
    32701 ggcaggttta aaagcattta tcaataatag ccaggaatag tcttatttac tttaaggata
    32761 ttgatgtcca ttcccgtact caacgcgcca gagagaaatt cacctggcgc gtatttttgt
    32821 tcgcggctta gctacggctg agcacgccgc ggcgatagag attacgttta atgcgcgtta
    32881 aaccaggttt tggcttacgc ggttcgtcca ggcttgccag gacaatctcc agcacacgct
    32941 ctgcgacatc gcggtgacgt tgagccactg ccagcaccgg gcactgtaag aagtcgagca
    33001 gttcgttatc gccaaaggtg gcaattgcca ggtcagaagg cagtttgccg tcacgacgta
    33061 gcgtgacatc catcacccct tgcaacaacg caaacgacgt tgtgaacagt gcctgcggca
    33121 tcggatgcgt ttccagccat ttttcgaata actgggcagc cgcctcccgc tcatagctgt
    33181 tggcataaag gaaatgcact tcgcgaggat catctttcca ggcagtacgg aaaccttgtt
    33241 cacgcaggaa gctgacagaa agctccggca gcgcgccaag ataaagtacc gtctcggcgg
    33301 gaaacttacg taactcttcc gccagcattt cggcatcatc ctgatcggca ccaaccacgc
    33361 tggtgaagtg ttcacgatcg agggcgcggt ccagcgcgac aatcgggaac gggtcgttag
    33421 cccagcgttg ataaaaagga tgctcaggtg gcaacgacgt cgaaacaata atggcatcaa
    33481 cctgacgctg taaaagatgc tcgatacaac gcatttcgtt gtcgggctga tcttccgagc
    33541 aggcaatcag cagttgataa cccctttgcc gcgcctggcg ttcaagatag ttagcgatgc
    33601 gggtatagct ggtgttctcc agatcgggga tcacaagacc aatagaacgt gtgcgtccag
    33661 cacgaagccc agctgccacg gcgttcgggt ggtaattgtg ctcacgcacc acagccatga
    33721 ctttttcaac ggttttgtcg ctcacacggt attgcttcgc tttgccgtta ataacatagc
    33781 ttgcagtggt ccgcgacact cccgccagcc gagcgatttc atccagtttc acaattgccc
    33841 cttgcgtaaa atgtaaaaac catagccatt gtactggtat gggttaaatt cattaacatc
    33901 taagcgcaga aaattaactg cggcaactgc ttttattcca ggttcccact gatttcgcaa
    33961 aaaaagccca acgtgacatg ttgggctgca aattgcgcat tgagatcatt ctcaacgcat
    34021 tattttatcg ccgcgcgaaa gtccgactac accagagcga gcaacttcca caattttcgc
    34081 cacatcgcga atcgatgcta aaaatgcatc aagcttatcg ctggtgcctg ctaattgaac
    34141 ggtataaagc gagggtgtga catcgataat ttgcccacgg aatatttccg tattacgttt
    34201 cacttcatca cgcccgtaac cgctggcctg aattttcacc agcatgattt cccgctcaac
    34261 gtgcgcgccc tgccccaact cgctcacgcg caagacatcg accagcttgt gtaattgctt
    34321 ttcgatttgc tcaagcactt tttcatcgcc cacggtctgg atggtcatac gcgataatgt
    34381 tggatcgtcg gttggcgcaa cggtcaggct ttcaatgttg tagccacgct gggaaaaaag
    34441 gccaatcacg cgggataacg cgcctgattc attttcgagt aagactgata atatccggcg
    34501 cataatcagg ttctctccgt tttgcttaac cacatttcat ccattccgcc cccgcgaatc
    34561 tgcatcgggt agacgtgctc actgccatcg acggtgacat caacaaacac caggcgatta
    34621 ttgcgcacct gttccagcgc ctcgctaagt ttgctttcca gttcttgcgg atgagagatc
    34681 tggatcccga cgtgaccata ggcttccgcc agacgaacga aatcgggtag tgactgcata
    34741 taagattgtg aatgacggcc ggaatagatc atgtcctgcc actgtttcac catccccaga
    34801 tagcggttat tgagattcac caccagtacc ggcaactcgt attgcaacgc tgtagacagt
    34861 tcctggatgt tcatctgaat actgccgtcg ccagtgacgc aaaccacggt ttcttctggc
    34921 aacgccattt tgacgcccag tgccgcaggt aaaccaaaac ccatcgtgcc gaggccgcca
    34981 gagttaatcc agcgacgcgg tttgtcgaat ggataataaa gcgcagcaaa catctggtgc
    35041 tgcccgacat cggacgtcac gtaagcgtcc ccattagtca accgccaaag agtctcgatc
    35101 accgcctgcg gtttaatctt ttcactgtga gtgtcatatt tcaggcactg acgagcgcgc
    35161 cactgttcaa tctgctgcca ccagtcgcgg atctcatcca gcggttgatg gacggattct
    35221 tgcgacagga gttcaagcat ttgttcgagg acctggcgag catcccccac aatcgggata
    35281 tcagcagtca cggttttaga aatggaagta ggatcaatat cgatatgcag aaccgtggca
    35341 tttgggcagt acttcgccag attgttcgtc gttcggtcat caaaacgtac cccgacggca
    35401 aaaatcacat ccgcgttatg catcgtcata ttggcttcgt aggtaccgtg cattcccaac
    35461 atgcccagtg cctgacgatg cgttgccgga aacgccccca gccccattaa tgaggaaaca
    35521 acgggcagat tcaacgcctc caccgtttct tttagctgct gatgacagcc cgccgtgatt
    35581 gccccaccgc ctacatagac aaccggtttt tttgccgcca ccagcgtttg cagagcgcgc
    35641 ttaatttgcc ctttatgccc ggaggtcgtg ggattgtaag aacgcatact gaccgactcc
    35701 ggccagacat agggtaattt gttcgccgga tttagaatat ctttcggtaa atcaacgacc
    35761 acgggtccag gacgaccact tgccgccagc cagaaagcct ttttcagcac ctgcggaatg
    35821 tcttccgttt gcttaaccag aaaactgtgt ttaaccaccg ggcgagaaat ccccaccatg
    35881 tcgcactcct gaaaggcatc gtagcctatc aacgaggtcg ctacctgccc ggaaaggaca
    35941 actaagggaa tggaatccat ataagcggtg gcaatgccag taatcgcatt ggtcgcccct
    36001 ggacccgacg ttaccagcac gacgccgact tccccggtcg cgcgcgccag accatcggcc
    36061 atatgcaccg ccgcctgctc atgacgaact aaaacatgat cgataccacc cacggtatgc
    36121 aatgcatcgt aaatatcaag gaccgcgcct ccgggataac cgaatacttg tttaacgccc
    36181 tgatcgataa gcgatcggac gaccatctcg gctccagaca acatctccat ggcctgcctc
    36241 actgtttgac ggaaaaaatg tgtaaaacac attaaccgtc cgatcttgct taagcaattg
    36301 gcagcctgag acataaacac ggggtaataa gaataaacag gaggaaaggt gaaaagaaac
    36361 gataaaatc
//
LOCUS       DAZKJR010000034        35999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKJR010000034 DAZKJR010000000
VERSION     DAZKJR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.034"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..1150)
                     /gene="ychF"
                     /locus_tag="ACHOWS_001735"
     CDS             complement(59..1150)
                     /gene="ychF"
                     /locus_tag="ACHOWS_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0047318.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEEREEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1267..1851)
                     /gene="pth"
                     /locus_tag="ACHOWS_001736"
     CDS             complement(1267..1851)
                     /gene="pth"
                     /locus_tag="ACHOWS_001736"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0047319.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEIWFTDGLTKATNRLHAFKAQ"
     gene            2129..2407
                     /gene="ychH"
                     /locus_tag="ACHOWS_001737"
     CDS             2129..2407
                     /gene="ychH"
                     /locus_tag="ACHOWS_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFQ0047320.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2462..4141)
                     /gene="dauA"
                     /locus_tag="ACHOWS_001738"
     CDS             complement(2462..4141)
                     /gene="dauA"
                     /locus_tag="ACHOWS_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFQ0047321.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4266..5213)
                     /gene="prs"
                     /locus_tag="ACHOWS_001739"
     CDS             complement(4266..5213)
                     /gene="prs"
                     /locus_tag="ACHOWS_001739"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0047322.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5364..6215)
                     /gene="ispE"
                     /locus_tag="ACHOWS_001740"
     CDS             complement(5364..6215)
                     /gene="ispE"
                     /locus_tag="ACHOWS_001740"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0047323.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGAEISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6215..6838)
                     /gene="lolB"
                     /locus_tag="ACHOWS_001741"
     CDS             complement(6215..6838)
                     /gene="lolB"
                     /locus_tag="ACHOWS_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFQ0047324.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPSNMELTDGGQRIKLKMDNWIVK"
     gene            7052..8308
                     /gene="hemA"
                     /locus_tag="ACHOWS_001742"
     CDS             7052..8308
                     /gene="hemA"
                     /locus_tag="ACHOWS_001742"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFQ0047325.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDGLGLE"
     gene            8350..9432
                     /gene="prfA"
                     /locus_tag="ACHOWS_001743"
     CDS             8350..9432
                     /gene="prfA"
                     /locus_tag="ACHOWS_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0047326.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9432..10265
                     /gene="prmC"
                     /locus_tag="ACHOWS_001744"
     CDS             9432..10265
                     /gene="prmC"
                     /locus_tag="ACHOWS_001744"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0047327.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10262..10654
                     /gene="sirB2"
                     /locus_tag="ACHOWS_001745"
     CDS             10262..10654
                     /gene="sirB2"
                     /locus_tag="ACHOWS_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFQ0047328.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10658..11467
                     /gene="sirB1"
                     /locus_tag="ACHOWS_001746"
     CDS             10658..11467
                     /gene="sirB1"
                     /locus_tag="ACHOWS_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFQ0047329.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11503..12357
                     /gene="kdsA"
                     /locus_tag="ACHOWS_001747"
     CDS             11503..12357
                     /gene="kdsA"
                     /locus_tag="ACHOWS_001747"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFQ0047330.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12506..12613)
                     /locus_tag="ACHOWS_001748"
     CDS             complement(12506..12613)
                     /locus_tag="ACHOWS_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFQ0047331.1"
                     /translation="MTLAQFTVIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13018..14118)
                     /gene="chaA"
                     /locus_tag="ACHOWS_001749"
     CDS             complement(13018..14118)
                     /gene="chaA"
                     /locus_tag="ACHOWS_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFQ0047332.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14388..14618
                     /gene="chaB"
                     /locus_tag="ACHOWS_001750"
     CDS             14388..14618
                     /gene="chaB"
                     /locus_tag="ACHOWS_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFQ0047333.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14776..15471
                     /gene="chaC"
                     /locus_tag="ACHOWS_001751"
     CDS             14776..15471
                     /gene="chaC"
                     /locus_tag="ACHOWS_001751"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFQ0047334.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(15515..15868)
                     /gene="ychN"
                     /locus_tag="ACHOWS_001752"
     CDS             complement(15515..15868)
                     /gene="ychN"
                     /locus_tag="ACHOWS_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFQ0047335.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16054..17448
                     /gene="ychO"
                     /locus_tag="ACHOWS_001753"
                     /gene_synonym="iatA"
     CDS             16054..17448
                     /gene="ychO"
                     /locus_tag="ACHOWS_001753"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFQ0047336.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDAFSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17449..18099)
                     /gene="narL"
                     /locus_tag="ACHOWS_001754"
     CDS             complement(17449..18099)
                     /gene="narL"
                     /locus_tag="ACHOWS_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFQ0047337.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18092..19888)
                     /gene="narX"
                     /locus_tag="ACHOWS_001755"
     CDS             complement(18092..19888)
                     /gene="narX"
                     /locus_tag="ACHOWS_001755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFQ0047338.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSARFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20227..21618
                     /gene="narK"
                     /locus_tag="ACHOWS_001756"
     CDS             20227..21618
                     /gene="narK"
                     /locus_tag="ACHOWS_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFQ0047339.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            22011..25754
                     /gene="narG"
                     /locus_tag="ACHOWS_001757"
     CDS             22011..25754
                     /gene="narG"
                     /locus_tag="ACHOWS_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFQ0047340.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLMGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQALDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITSQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            25751..27289
                     /gene="narH"
                     /locus_tag="ACHOWS_001758"
     CDS             25751..27289
                     /gene="narH"
                     /locus_tag="ACHOWS_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFQ0047341.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            27286..27996
                     /gene="narJ"
                     /locus_tag="ACHOWS_001759"
     CDS             27286..27996
                     /gene="narJ"
                     /locus_tag="ACHOWS_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFQ0047342.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            27996..28673
                     /gene="narI"
                     /locus_tag="ACHOWS_001760"
     CDS             27996..28673
                     /gene="narI"
                     /locus_tag="ACHOWS_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFQ0047343.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(28858..28989)
                     /locus_tag="ACHOWS_001761"
     ncRNA           complement(28858..28989)
                     /ncRNA_class="other"
                     /locus_tag="ACHOWS_001761"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(29036..29120)
                     /locus_tag="ACHOWS_001762"
     tRNA            complement(29036..29120)
                     /locus_tag="ACHOWS_001762"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29084..29086),aa:Tyr,seq:gta)
     gene            complement(29280..30122)
                     /gene="purU"
                     /locus_tag="ACHOWS_001763"
     CDS             complement(29280..30122)
                     /gene="purU"
                     /locus_tag="ACHOWS_001763"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFQ0047344.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(30172..30630)
                     /gene="ychJ"
                     /locus_tag="ACHOWS_001764"
     CDS             complement(30172..30630)
                     /gene="ychJ"
                     /locus_tag="ACHOWS_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFQ0047345.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAATLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GSKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            30743..31648
                     /gene="rssA"
                     /locus_tag="ACHOWS_001765"
     CDS             30743..31648
                     /gene="rssA"
                     /locus_tag="ACHOWS_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFQ0047346.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            31740..32753
                     /gene="rssB"
                     /locus_tag="ACHOWS_001766"
     CDS             31740..32753
                     /gene="rssB"
                     /locus_tag="ACHOWS_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFQ0047347.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            32955..33863
                     /gene="galU"
                     /locus_tag="ACHOWS_001767"
     CDS             32955..33863
                     /gene="galU"
                     /locus_tag="ACHOWS_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0047348.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(34008..34421)
                     /gene="hns"
                     /locus_tag="ACHOWS_001768"
     CDS             complement(34008..34421)
                     /gene="hns"
                     /locus_tag="ACHOWS_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFQ0047349.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            35025..35642
                     /gene="tdk"
                     /locus_tag="ACHOWS_001769"
     CDS             35025..35642
                     /gene="tdk"
                     /locus_tag="ACHOWS_001769"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0047350.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(35925..>35999)
                     /locus_tag="ACHOWS_001770"
     CDS             complement(35925..>35999)
                     /locus_tag="ACHOWS_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047351.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         8864 a         8846 c         9440 g         8849 t
ORIGIN      
        1 ccattatgtg cgactcacat aatggctcat tattaaccgt cagggtatta cgtggaattt
       61 agacgttgaa caggaagttc atcacatcgc catctttaac gatgtaatct ttaccttctg
      121 cacgcatttt gcctgcttct ttcgcgcctt gttcaccttt gtaagtgatg aaatcttcaa
      181 acgagatagt ctgtgcgcgg ataaagcctt tttcgaaatc ggtatggatt ttgccagccg
      241 cttgcggtgc agttgcgccg acggggatgg tccatgcgcg cacttctttc accccagcgg
      301 tgaagtaagt ttgcaggttc agcagtttat aaccggcacg gatcacacgg ttcaggcccg
      361 gctcttccag tcccagttcc tgcataaact cttcacgttc ttcatcgtcc agttcggcaa
      421 tgtctgcttc aacagctgca caaaccggaa ccacaacaga accttctttc gccgcgattt
      481 cacgcacctg gtcaagatac gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt
      541 acatcgtcgg tttcagcgtc aggaagctca ggtaacgaat agccgctttt tcttctgcgc
      601 ttaaatccag cgcgcgcagc atgcccgcgt tttccagctg tggaaggcat ttttccagta
      661 ctgccagttc ggctttcgcg tctttatcgc cgcctttggc tttcttctgt acgcggtgaa
      721 tcgcgcgctc gcaggtgtcg agatccgcca gtgccagttc ggtattgata acttcaatat
      781 cgtccgccgg gttaacttta ccggaaacgt gaatgatatt gtcattctca aagcagcgaa
      841 caacgtggcc gatcgcttcg gtttcacgaa tgttggtcag gaactggtta cccagacctt
      901 cacctttcga cgcgcccttt accagaccgg cgatatcgac aaattccatg gtagtgggaa
      961 gcgtacgctg cggttttacg atttcagcca gttgatccag gcgagggtca ggcattggta
     1021 cgacgcccgt gttcggctca atggtacaga atggaaagtt ggccgcttca ataccggctt
     1081 tggtcagcgc gttgaacagg gtagatttcc cgacgttggg caaaccgacg ataccgcatt
     1141 tgaatcccat gatttaaatc accttaatat cttaataatc aacctgttat tgctaacaga
     1201 ttgcagaaat ggaaataact ttgcctatta tacacggcac tcggcaaaaa tgccgcagac
     1261 aacgacttat tgcgctttaa aggcgtgcaa tcggttcgtt gctttggtca agccatctgt
     1321 aaaccatatt tcagtacaac gtgccgcttc gtcgatggct tcatcaatta acttctgttc
     1381 actaacaggc ggtttgccta acacaaaacc gacaacttta tttttatcgc ctggatgacc
     1441 aattccgatg cgtaaacggt gaaagttagg gttattaccc aatttactga tgatgtcttt
     1501 cagtccattg tgaccaccat ggccaccgcc caacttaaat ttggcgacgc caggaggcag
     1561 atccagttcg tcgtgggcca ccagaatctc gtccggatta atgcggaaaa aactggccat
     1621 cgccgcaacg gctttgccgc tgagattcat aaatgtagtc gggactaaca ggcggacatc
     1681 ttcgcctcca agggtgactc gcgaagtata accaaagaat ttagcctctt cgcgcagcgg
     1741 agcgcgcaat cgctctgcca gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc
     1801 ggcgtattca gcgccggggt tcgccaggcc gacaatcaat ttaatcgtca cgtttttttg
     1861 tcctgagtgt gtacataact ggcgcgtagt ttactgtttg cggccccgct tgacaaaaaa
     1921 ctgcgtatca aatgcagata acgcaataat tgcctaagtg gactattaga aagtcaaggt
     1981 gttcaggcgt ttatttgtaa agttttgttg aaataagggt tgtaattgtg atcacgcccg
     2041 cacataaccc acagggtgtt gtctatactt tacacataag gaagaggggt attccctgtt
     2101 acaacccaga aagttccgga ggtgacatat gaaacgcaaa aacgcttcgt tactcggtaa
     2161 cgtgctcatg gggttgggtc tggtggtaat ggtggtcggc gtggggtatt caatcctcaa
     2221 ccagttacca cagtttaata tgccccagta tttcgcacat ggtgcagtgc taagtatttt
     2281 cgtcggtgcc attctctggc tggcgggtgc ccgtgttggc gggcatgaac aggtgtgcga
     2341 ccgttactgg tgggttcgcc actatgacaa acgttgccgc cgtagcgata atcgccgtca
     2401 tagctaacaa ataatgcggt ttgctgacca gtcagtttgc gctgactggt caatttcgta
     2461 cttataaatc cgccatcgcc gcgcgacgat tcgggaagaa cgccaggcgt cccgggatcg
     2521 gttgaatgcc tgcgcgagcc atagtgcgca gtggctggaa ttccacgttg cacacgcgca
     2581 gttcacatcc ttcgggtaga cgcttcacaa aacgctggaa cgcatcaaga ccaccagcat
     2641 caagtaccgg aacggcatcc cacttcagaa tcacaatccg tttgccttca agacgtgact
     2701 ccaggtccgt gaacaagcct tcagcggcag caaagaacag cgggccaata acgcgcagaa
     2761 ccagaacatc gtctggaacg tctacgacta ccggtgccag gcgagtcata cgtgcgatac
     2821 gacgcataaa cagtagcgat gccagcacga tccccacgct gatggcaata accatatcaa
     2881 acagcacggt cagcgacatg cacagcagca tgacgatgat gtcatctttc ggcgcatgac
     2941 gcagcaagtc gaccactttg tgcgcttcac tcatgttcca cgccaccatc aacagcaggg
     3001 cagccatggc ggaaagaggc agccaggaga gcagcggtgc cagtaccagc agggcaagaa
     3061 taaccagaat agagtggatc accgccgaga taggggaagt tgccccggca cggacgttag
     3121 cggcagaacg cgcgatggca gctgtagcgg taataccacc aaagaacgga gcgatgatat
     3181 tccccagtcc ctgtccaacc agttcactgt tcgctttgtg tttcgtcccg gtcataccat
     3241 ccagcaccac ggcgcagagc agtgattcaa ttgcccctaa catcgccatc gaaaatgctg
     3301 ccggaagcag agtgcgaatg gagtcccagg tgagggtaaa ttcggaattg ggcaggtccc
     3361 acggcagtac cagttgcggc agcagttgcg gaataccgtt accctgagaa ccatcggcca
     3421 gaacgtagtg gaattgcgaa ccgatggtag caacatgtcc gccgagcagg ttaacaatcc
     3481 ccatcaccgc gcaaccagcc agcaaggccg gaaggtgacc gggtaaacga atgcccagac
     3541 gtggccagaa aaccagaatg ccaagcgtca caatgccaat ggcagcatca cccacattaa
     3601 tggtcggcaa cgccataaat aatgcgccga ctttttgtag ataatgttca gggacatggg
     3661 ccatttgcag accgagaaaa tctttaatct gcatggtacc gatggtgatc ccgataccgg
     3721 aagtaaaacc taaggtaacg gaaaccggaa tatattcaat caagcgtcca aaacgtgcca
     3781 gccccatcag gatcaaaaag acacctgaca tgagtgtcgc gaccagcagt cctgccagtc
     3841 caaactgttg cgaaacggga tagagaatta ccacaaatgc cgcagtcgga ccggaaacgc
     3901 taaagcgtga cccacccgtc agagcaatga caatccccgc aacagctgcg gtatataaac
     3961 cgtactgggg tgccacacca ctaccaatag ccaacgccat tgccagcggg atagcaataa
     4021 tcccgacggt tatcccggca atcaggtcac gggtaaaccg tgcggcagta tatttttctt
     4081 tccagcaagc gtcgatcaga gcgcggaaag gcatcacatg tgaggaaaat attttgttca
     4141 caataatgtt tcatccgtga gcgcatcatc tgtcaactaa atggcaggtg aaggaggcat
     4201 aggtcataca aatggatatt acagacaaaa aaacccgccg cagcgggtct ttgagccggg
     4261 ttcgattagt gttcgaacat ggcagagatc gattcttcgt tgctgatacg acgaatcgct
     4321 tcggccagca tacctgacag ggtcagagta cgcacgttcg gcagtgattt gatttcatcg
     4381 ctcagcggaa tggtatcgca gacaacgact tcatcaatta cagagttacg caggttgttc
     4441 gccgcgttgc cagagaagat cgggtgagtc gcgtacgcaa atacacgttt agcaccacgt
     4501 tctttcagag cttcagcagc tttacacagc gtaccgccag tatcgatcat atcatcgacc
     4561 agtacgcagt cacgacctgc aacgtcaccg atgatatgca tcacctggga aacgttcgca
     4621 cgcggacgac gtttgtcgat gattgccata tcggtatcgt tcagcagctt agcgatagcg
     4681 cgggcacgca caacgccgcc gatgtccgga gaaaccacaa ttgggttatc cagattcagc
     4741 tgcagcatgt cttccagcag gatcgggcta ccaaatacgt tatcaaccgg aacgtcgaag
     4801 aaaccctgaa tctgttcagc gtgcagatcc actgtcagca cacggtcaac accgacgctg
     4861 gagaggaagt ctgcaaccac tttcgcagtg attggtacac gagcggaacg gacgcgacgg
     4921 tcctggcgcg catagccaaa gtaggggata acagcggtga tacgacctgc ggaagcacga
     4981 cgcagggcat caaccataac gactaattcc atcaggttgt cgttagtagg ggcacaagtg
     5041 gactggatga tgaaaatatc accaccgcgt acattttcgt taatttgtac gctgacttcg
     5101 ccgtcgctaa agcgacctac agcggcgtcg ccgagtgaag tgtacaggcg gttggcaata
     5161 cgttgtgcta gttccggggt ggcgttacca gcaaaaagct tcatatcagg cacgagaaga
     5221 acctcaggca tgcgtccatt ggtggaaaga atctgccgaa aactgtgcgg gccaggcatg
     5281 attctttcca ggcggtgtat taaagagcgc gatgcaacgt ctggaacaag gtgacgttgt
     5341 caccgaaact cagcttgccc ggcttaaagc atggctctgt gcaatgggga aagattaacg
     5401 cctttcgcca caaagccatt gagccattcc ggggcttgct ctagcacctg gcgggcttca
     5461 gactctgtat caaattcagc aaagacacag gcccctgtcc cagtcaggcg cgacggggcg
     5521 tattctaaca gccaggaaag caccgcatca acctcgcgaa aacgttttct tgcgataacc
     5581 tcgcaatcat tgctgaattc acattttagc aacgtttcta ttgacctttt tggcgtattg
     5641 cgcgggagtt caggatcttt aaaaatcacc ggtgtcggaa tacttacacc agggtgcgcc
     5701 accaagtacc acttctctgg cggatccacc ggcgttagta tttcaccaac gccttcggca
     5761 aacgcggcat gcccccgaac aaagacagga acatctgcgc ccagcgtcag ccccatttcc
     5821 gccagctcat ccatgcttag cccgcattgc cagagatggt ttaatgccac cagcaccgtc
     5881 gcggcattgg atgaaccacc acccagaccg ccgcccatcg gcaaacgctt gtcaatactg
     5941 atttctgcac cgcttcccgt aggaagacgc ccgctgtctg ccgctgtttt catcagcaac
     6001 cgtgctgcgc gaacgatcag gttatcttca tgttccacgc cttcaacggg tgttaacaga
     6061 cgaatatccc catcgtcacg aagctcaatg ctgatggtgt cgccgtaatc caggaactga
     6121 aacagcgttt gcagcgtgtg gtaaccgtcc gcacgctgac cggtaatgta taaaaacaga
     6181 ttaagttttg ccggagaggg ccactgtgtc cgcattattt cactatccag ttatccattt
     6241 ttaacttgat gcgctgacca ccatcggtga gttccatatt ggatggcatc gcaggttgag
     6301 tttttgtgtc ataaccaccg taaacaactt tccagttttt gccattctgg ctgtaggtga
     6361 tttcgctaag gcggtactgg tcgtccagtt tgtagtcagt cgcatcaccc ggcaaaccta
     6421 aaatccactg gcgtaagctg ttgagtggaa tgggcattcc ggtcaatttg ccaatcatct
     6481 cttcggcgtc atcggcggta taacgctggc ctttattgtc gactaactgc acgttacccg
     6541 gttgagcatt cagctccagt tccgtgctgc ccaatgggtt agtcagcagc agacggtagc
     6601 gatcctggcc agtttgctgc cagaagaaac gggcgtacac tttttgttgg tcagaaatat
     6661 aagcgaacgc gccgcgagtt tgatactgat taagattgcg cacgtcttgc tgatgctgac
     6721 gccattgtgg cgaatccggg cttttgccag gacctttggg cgtggtaacg gaacaggcag
     6781 tgagcacaag agcagccagc ggtagtaggc ggataagacg aaaatcgggc aggggcatag
     6841 tgatgacaag tccttgagat acgttgcagt tataaccctt aatgctagcg ttaccgtccg
     6901 ctatcgtcta tgttcaagtt gtcttaattg ccagaatcta acggctttcg gcaattactc
     6961 caaaaggggg cgctctcttt tattgatctt acgcatcctg tatgatgcaa gcagactaac
     7021 cctatcaacg ttggtattat ttcccgcaga catgaccctt ttagcactcg gtatcaacca
     7081 taaaacggca cctgtatcgc tgcgagaacg tgtatcgttt tcgccggata agctcgatca
     7141 ggcgcttgac agcctgcttg cgcagccgat ggtgcagggc ggcgtggtgc tgtcgacgtg
     7201 caatcgcacg gaactttatc ttagcgttga agagcaggat aacctgcaag aggcgttaat
     7261 ccgctggctt tgcgattatc acaatcttaa tgaagaagac ctgcgtaaaa gcctctactg
     7321 gcatcaggat aacgacgcgg ttagccattt aatgcgtgtt gccagcggcc tggattcact
     7381 ggttttgggg gagccgcaga tcctcggtca ggttaaaaaa gcgtttgccg attcgcaaaa
     7441 aggccatatg aaggccagcg aactggaacg catgtttcag aaatctttct ctgtagcgaa
     7501 acgtgttcgc actgaaacag atatcggtgc cagcgctgtt tctgtcgctt ttgcggcttg
     7561 tacgctggcg cggcagatct ttgaatcgct ctctacggtg acggtactgc tggtgggtgc
     7621 gggcgaaacc atcgagctgg tggcgcgtca tctgcgcgaa cacaaagtac agaagatgat
     7681 tatcgccaac cgcactcgcg aacgtgccca aattctggca gatgaagtcg gcgcggaagt
     7741 tattgccctg agtgatatcg acgaacgtct gcgcgaagcc gatatcatca tcagttccac
     7801 cgccagcccg ttaccgatta tcgggaaagg catggtggag cgcgcattaa aaagccgtcg
     7861 caaccaacca atgctgttgg tggatattgc cgttccgcgc gatgttgagc cggaagttgg
     7921 caaactggcg aatgcttatc tttatagcgt ggacgatctg caaagcatca tttcgcacaa
     7981 cctggcgcag cgtaaagccg cagcggttga ggcggaaact attgtcgctc aggaaaccag
     8041 cgaatttatg gcgtggctgc gtgcacaaag cgccagcgaa accattcgcg agtatcgcag
     8101 ccaggcagag cacgttcgcg atgagttaac tgccaaagcg ttagcggccc ttgagcaggg
     8161 cggtgacgct caagccatta tgcaggatct ggcatggaaa ctgactaacc gcctgatcca
     8221 tgcgccaacg aaatcacttc aacaggccgc ccgtgacggg gataacgaac gcctgaatat
     8281 tctgcgcgac ggcctcgggc tggagtagca gtacatcatt ttcttttttt acagggtgca
     8341 tttacgccta tgaagccttc tatcgttgcc aaactggaag ccctgcatga acgccatgaa
     8401 gaagttcagg cgttgctggg tgacgcgcaa actatcgccg accaggaacg ttttcgcgca
     8461 ttatcacgcg aatatgcgca gttaagtgat gtttcgcgct gttttaccga ctggcaacag
     8521 gttcaggaag atatcgaaac cgcacagatg atgctcgacg atcctgaaat gcgtgagatg
     8581 gcgcaggatg aactgcgcga agctaaagaa aaaagcgagc aactggaaca gcaattacag
     8641 gttctgttac taccaaaaga tcctgatgac gaacgtaacg ccttcctcga agtccgtgcc
     8701 ggaaccggcg gcgacgaagc ggcgctcttc gctggcgatc tgttccgtat gtacagccgt
     8761 tacgccgaag cccgccgctg gcgtgtagaa atcatgagcg ccagcgaggg tgaacatggt
     8821 ggttataaag agatcatcgc caaaattagc ggtgatggtg tatatggtcg tttgaaattc
     8881 gaatctggcg gtcatcgcgt gcagcgtgtg cctgctacgg aatcgcaggg tcgaattcat
     8941 acttccgctt gtaccgttgc ggtaatgcca gaactgcctg acgcagaact gccggacatc
     9001 aacccagcag atttgcgcat tgatactttc cgctcgtcag gggcgggtgg tcagcacgtt
     9061 aacaccaccg attcggcaat tcgtattact cacttgccga ccgggattgt tgttgaatgt
     9121 caggacgaac gttcacaaca taaaaacaaa gctaaagcac tttctgtact cggtgctcgc
     9181 atccacgctg cagaaatggc aaaacggcaa caggccgaag cgtctacccg tcgtaacctg
     9241 ctggggagtg gcgatcgcag cgaccgtaac cgtacttaca acttcccaca ggggcgcgtt
     9301 accgatcacc gcatcaacct gacgctctac cgcctggatg aagtgatgga aggtaagctg
     9361 gatatgctga ttgaaccgat tatccaggaa catcaggccg accaactggc ggcgttgtcc
     9421 gagcaggaat aatggaatat caacactggt tacgtgaagc aataagccaa cttcaggcga
     9481 gcgaaagccc gcggcgtgat gctgaaatcc tgctggagca tgttaccggc agagggcgta
     9541 cttttattct cgcctttggt gaaacgcagc tgactgacga acaatgtcag caacttgatg
     9601 cgctactgac gcgtcgtcgc gatggtgaac ccattgctca tttaaccggg gtgcgagaat
     9661 tctggtcgtt gccgttattt gtttcgccag cgaccttaat tccgcgcccg gatacggagt
     9721 gtctggtgga gcaggcactg gcgcggttgc ctgaacaacc ttgccgtatt ctcgatctcg
     9781 ggacgggtac cggggcgatt gcgctggcgc tggccagcga gcgtccggac tgcgaaatta
     9841 ccgctgtaga tcgtatgcca gatgctgtcg ccctggcgca acgtaatgcc cagaatctgg
     9901 cgatcaaaaa catccatatt ctgcaaagcg actggtttag cgcgctagcc gggcagcagt
     9961 ttgcgatgat tgtcagtaat ccgccgtata ttgacgagca ggacccacat cttcaacaag
    10021 gcgatgtccg ctttgagccg ctcactgcgc tggttgcggc agacagtgga atggcagaca
    10081 tcgtgcatat catcgaacag tcgcgtaacg cgctggtatc cggcggcttt ctgcttctgg
    10141 aacatggttg gcagcagggc gaagcggtgc gacaggcatt tatccacgcg ggatatcatg
    10201 acgtcgaaac ctgtcgggac tatggtgata acgagcgcgt aacgctcggc cgctattatc
    10261 aatgacaagt ttttctacac tgcttagtgt tcatcttatt agtatcgcgc tttcagttgg
    10321 gctattaacc ttacgtttct ggctacgtta tcagaagcat ccacaggcat ttgcgcgctg
    10381 gacgcgcatt gtgccgccgg ttgtcgatac gctgttattg ttaagcggca ttgcgttgat
    10441 ggctaaagcg cacatcctgc cattttccgg gcaggcacag tggctgactg aaaagctgtt
    10501 tggagttatc atttatatcg ttttgggttt tattgcactc gattatcgtc gtatgcacag
    10561 tcagcaggcg cgcattattg ccttcccgct ggcgttggtg gtgctgtaca tcatcattaa
    10621 actcgccacc acaaaagtac cgttactggg gtaagtcatg agatcgttag ctgatttcga
    10681 atttaataaa gcgccattgt gcgaaggcat gatcctggct tgcgaagcaa tccgccgcga
    10741 ttttccctcg caagatgttt acgacgaact ggagcgtctc gttagtctgg cgaaggaaga
    10801 aatcagccag cttctgcctt tagaagagca gctggaaaaa ctgatcgcgc tgttttacgg
    10861 cgactgggga tttaaagcct cacgcggcgt ttatcgtctt tccgatgcat tatggctgga
    10921 ccaggtgtta aagaatcgac agggcagtgc ggtatcatta ggtgccgttt tattatgggt
    10981 cgcgaatcgt cttgatttgc cgctgctgcc ggtgattttc cctacgcagc tgatattgcg
    11041 cattgaatgt ccggatggcg aaatttggct gattaatcct tttaacggtg aatcgttaag
    11101 cgaacatatg ctggacgtat ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta
    11161 tgaagacctt gatgaagctg ataacattga ggtaatccgc aaattgctgg atacactcaa
    11221 agcttcgttg atggaagaga atcagatgga gctggcgtta cgcaccagcg aagctttatt
    11281 acaattcaac ccggaagatc cctatgaaat tcgcgatcgc gggttgattt atgcgcaact
    11341 ggattgcgaa cacgttgcgt tgaacgattt aagttatttc gttgaacagt gtccggaaga
    11401 cccgatcagc gaaatgatcc gtgcgcaaat aaataacatc gcgcataaac atattgtgct
    11461 gcattaatta atcgacattt tactcaagat taaggcgatc ctatgaaaca aaaagtggtt
    11521 agcattggcg acatcaacgt agcaaatgac ctgccgttcg tactgtttgg cggtatgaac
    11581 gtgctggaat ctcgcgatct ggcgatgcgc atttgcgagc actacgtaac cgtgacccag
    11641 aaactgggta tcccttacgt gttcaaagcc tcttttgaca aagccaaccg ctcctccatc
    11701 cactcttatc gtggaccggg cctggaagaa gggatgaaaa tcttccagga gttgaagcag
    11761 acttttggtg tgaaaattat caccgacgtt cacgaaccaa gtcaggcaca gcccgttgcg
    11821 gatgtcgtgg atgtgattca gttgccggcg tttcttgctc gccagactga cctggtggaa
    11881 gccatggcga aaaccggtgc ggtaattaac gtcaagaaac cgcagtttgt tagcccggga
    11941 cagatgggta atatcgttga taaatttaaa gaaggcggca acgaaaaagt gattctttgc
    12001 gatcgcggtg ctaacttcgg ctatgacaac ctggttgtcg atatgctggg cttcagcatc
    12061 atgaagaaag tgtctggtaa ttcgccggtg attttcgacg tgacccacgc actgcaatgc
    12121 cgcgatccgt ttggcgcagc ttccggcggt cgtcgtgctc aggtggctga gctggcacgt
    12181 gccggtatgg cggtaggtct ggcggggctg tttattgaag cgcatccgga tccggaacat
    12241 gcgaaatgtg atggtccatc cgcgctgccg ctggctaaac tggaaccgtt cctcaagcag
    12301 atgaaagcga ttgatgactt ggtgaaaggt ttcgaagaac tggataccag caagtaatct
    12361 tttttgcctg aaaactaaag ccttagcgtt ctgcgttaag gcttttttca tgggggccgg
    12421 atacaaaaag ggccgcaggc tgttacccct gcggccggtt tcgggcgcat attgccatca
    12481 cggcagcctg acgcccgttt tcgccttact tccggttacg ccaccagccg acaatcgctg
    12541 cggtaataat tcccgccagg atcggtgctg ccaggtcgtg ccagaaaatc acggtaaact
    12601 gcgcgagcgt catatagccg ccttgttgta atgacaacat tttgcggcta ttcttgaatt
    12661 gttctggttc aagattagcc cccgttctgt tgtcaggtta tacctctcaa cgtgcggggg
    12721 ttttctcttt ccagcaacca atgccaccag ggataaagcc cccgcaacat tgcgcctcac
    12781 cggataacgc cggcttggtg tggatactac gactcaattc atcttcactt catccctgaa
    12841 atgtttgcaa tgaagagtgc attccggttt ttcaacagct gttacagtcg tttcgtgcag
    12901 gttgtggttc aggctcgcaa ttctgattaa caacctgtcc aggtagtatg aagaaataca
    12961 ataagcagga atttatatta tctcccccga tttccgtcat cggaggagat ataactgtca
    13021 ggcaaatatc gtcatcaaat aggcggcaaa cagtgccaga tgcgctgcgc cattgagcac
    13081 gttagtacgt ccggtggaga aggagatatg acacagcact aaagaggcca ccatcaccac
    13141 catttctggc gcaccaagtg caaactgcaa ttcgttaccc gtcataaagg caattagcgt
    13201 gacgacaggt acggtaagcg aaatggttgc taacactgaa ccaaagaaca gattcatcgc
    13261 gcgctggacc tggttgttca acactgcttt taacgcacct aaaccttccg gcgacagaat
    13321 caacagagcc accaggaagc cagtaaaggc gacaggggca ttcatgctgt cgagcaatgt
    13381 ctccagcggg ctggcgttca ttttggtcac cgcaataacg gcaatcagat ggacaatcaa
    13441 ccagatagca tgccacaggc tgctatgggc agacggttta ccgtgatgcg ggtcgtcgtc
    13501 atcactgtca tcttcgtgct cgtagacaaa caaactttga tgcgttttgg tctggatcag
    13561 caaaaatacg ccatacattg ccgcagaaat taatgctacc agtaacgcct gaccggttga
    13621 aaaattcgcc gcaggcagag ccattggaaa taccagtacg attatcgcca gggggaacag
    13681 cgcaattaaa tactgcttga taccaaacag attcatatat tgggtggcaa acttgcgtcc
    13741 gcccaacaat aatgaaaagc caaccagccc accggtaaca atcataatga ttgaatagag
    13801 cgtatcacgc attagcgttg gcgcggcgtc gccggttgcc attaaagctg aaatcaaact
    13861 gacttcaaga ataaccactg aaaggctaag aataagcgaa ccgtaaggct ctcccaggcg
    13921 atgggctaat acgtccgcat gacggacaac actaaaggcg ctacttaaaa taccaataag
    13981 agcaagaaga ttgatggcaa tgaccactgg tagtgtctgg ctgcttcccc acaggaacag
    14041 cactaccagc gccagaaccg ggaaaataag cgaagtctcc ttgtggcggg tttttaccgc
    14101 ctcttgagca tttgacatta tggttatccc tttgcagatg aatttatcga aaatgtaaaa
    14161 aataggtagg aaaaataaca gaaagtgtct ggatatcggt aacattttac gaatttttac
    14221 cccgctgtcg attttttact catttgggca taaaaataag taatacgttt agacaatgtt
    14281 tgttttagca cttcttaaga agagtctgac atgaaaattc ttatgttttg gcaagagtag
    14341 atattgttga ccacacttaa tgttcaactt tgtaaaagga gtcaacgatg ccgtataaaa
    14401 cgaaaagcga tctgccggaa agcgtaaagc acgtgcttcc gtctcatgcc caggatatct
    14461 ataaagaagc gttcaacagc gcatgggatc aatataaaga taaagaagat cggcgtgatg
    14521 acgccagtcg cgaagaaaca gcgcataaag tggcctgggc tgctgtgaag catgaatatg
    14581 ccaaagggga tgatgataaa tggcataaaa aatcgtaaaa ccggtagctt agttaaagct
    14641 attcgtgcgg tgttgccttg caagtggtcc gtggattgca tattgtcccg ttagtggttt
    14701 caaaatgagc agtaaaaatg tccggaagac accaaaaagt tgtcgcaggg aagtatgcag
    14761 tggcggaggt gtaaggtgat aacgcgtgat ttcttgatga atgccgattg taaaacggca
    14821 tttggtgcca ttgaagaatc actcttatgg tcagcagaac aacgggcggc ttcgctggcg
    14881 gcgacgctgg cttgtcgacc tgatgaggga ccggtgtgga tcttcggtta tggatcgttg
    14941 atgtggaatc cggcactgga gtttaccgag tcgtgcaccg gtacactggt tggatggcat
    15001 cgtgcgtttt gcctgcgcct gaccgccggg cgcgggactg cgcaccagcc gggacggatg
    15061 cttgcactga aagagggcgg acgcaccaca ggcgtcgcct atcgactgcc agaagagacg
    15121 ctggagcagg aactaaccct gttgtggaag cgcgagatga ttaccggctg ttatctgcca
    15181 acctggtgtc agcttgatct tgatgatgga cgcacagtaa acgccattgt gtttattatg
    15241 gacccgcgac atccagaata tgaatctgac actcgcgctc aggtcatcgc gccgttgatt
    15301 gcggcggcga gcggtccact gggaaccaac gcacaatacc tgttttcact ggaacaggag
    15361 cttatcaaac tgggaatgca ggacgatggg ctgaatgatt tgctggtatc ggtaaaaaaa
    15421 ctgctggcgg agaattatcc ggatggtgtg ttacggccgg gactcgcctg agtaaacttc
    15481 cctcatagtg gggcgtcaga cgccccctca aacattaaaa tgtgagcact ttatcggctg
    15541 acagcgtcca ttgcgccagt tccacaagcg taccgatttc caccccatca atcagaggaa
    15601 gtgtactaat cccgcgcccg tcggtacagg ttttgcacaa tttcaccggt acattctgag
    15661 cggtaaggat ctccagcatt tgctgaatgt tgtagccttc ccctggtttt tgcccgcgca
    15721 acccggctgt gaccgcatca gacatcagga acagacgcag atccagattg ctctcctgct
    15781 ctcgtaacgc aatggccagc cgcaagctgt taaataagga ttcgctcccg taaggtgcgc
    15841 cattggcaac gatcacgatt ttttgcatta tttactcctg tattcaggga attagacact
    15901 catcttctat cttactgctt ctgcagcgtc tgaccaatcg gtcacatttt taaggatttt
    15961 cctgaaagcg cgagaaaata cgacaaaagt tgccagtaat cgttattctt tagggctatg
    16021 gtttttcatt ttttaccgga agttaccgac gttttgagcc gtttcgttcc tcgcattatt
    16081 ccgttttatt tactcttgct ggcggcaggc ggtacagcta acgcacaatc taccttcgag
    16141 caaaaagcgg caaatccctt tgataataac aatgatggtc tgccggattt aggcatggca
    16201 cctgaaaatc atgatgggga aaaacacttt gctgaaattg tgaaagattt cggcgaaacc
    16261 agtatgaatg ataacgggct ggatactggc gagcaggcaa aagctttcgc attgggaaaa
    16321 gtccgcgacg cgtttagtca acaggttaat cagcacgtag agtcgtggct atcagcgtgg
    16381 ggaaatgcca gtgttgacgt caaagtggat aacgaaggac atttcaccgg cagtcgtgga
    16441 agctggtttg tgccgttaca agataatgat cgttatctca cctggagcca gcttggtctt
    16501 actcagcagg atgatgggct ggtgagcaat gtgggcgttg ggcaacgctg ggcgcgcggc
    16561 agctggctgg tgggttataa cactttttat gacaacttgc tggacgaaaa tcttcagcga
    16621 gcgggcttcg gtgccgaagc gtggggcgaa tatttgcgac tatcggcaaa cttttatcag
    16681 ccgtttgctg catggcatga acagacagcc acgcaggaac aacggatggc gcgcgggtac
    16741 gacctgacag cccggatgcg catgccgttt tatcaacacc tcaataccag tgtcagcgta
    16801 gaacagtatt ttggtgatcg tgttgatttg tttaactctg gtacgggtta tcacaatccc
    16861 gtcgcgttga gtctgggatt aaattatacc ccagtgccat tagtcactgt gacggcccag
    16921 cataaacagg gtgaaagtgg cgaaaatcaa aataacctcg ggctgaatct taattaccgc
    16981 tttggtgtac cgctcaaaaa acaactttct gcgggcgagg ttgccgaaag tcagtcgtta
    17041 cgtggtagtc gctatgacaa tccgcagcga aataatctac cgactcttga gtaccgacag
    17101 cgaaaaacgt taacggtgtt tctggcgaca ccgccgtggg atctaaaacc tggcgaaaca
    17161 gtgccgctga aattacaaat ccgcagtcgt tacggtattc ggcaactgat ttggcagggc
    17221 gatacgcaga tattaagttt gacgccgggc gcacaagcca acagtgagga gggctggacg
    17281 ctgatcatgc ctgactggca aaacggggaa ggcgcaagca atcactggcg attgtcagtg
    17341 gtggtggaag ataaccaggg gcagcgtgtc tcctccaatg agatcacgct aacgcttgtc
    17401 gaaccgttcg acgcattgtc aaacgacgaa ctgcgctggg aaccgtaatc agaaaatgcg
    17461 ctcctgatgc acccataccg cagcttccac gcgagacttt agcttcattt tcttcagcat
    17521 gtgcttgacg tgaactttta ctgtgctttc ggtgatgtcc aggcggcggg caatcatctt
    17581 gttcggcaaa ccctgggcaa ttagcttgag aatatcgcgc tcgcgtgggg ttaactggtt
    17641 aacatcgcgc tcagtagtgg cacggttagc gcgcaagctg gcggccagaa caggcgttaa
    17701 tgcttcgctt aataccattt cgccagcagc tgcctgatgt aacgctttca gcagatcttc
    17761 cggttccata tcttttaaca gatagccatc cgcgccgcgt ttcagtgcgg tgaccacatc
    17821 ttcttcatgg ttagagacgc tgaataccac aatgcgcccg gagagggact tttcgcgcag
    17881 tttatccagc gtttccagac cgttcatgcc gggcatattg agatctaaca ggatcagatc
    17941 gggatcaaga gactccgcca gttcaatacc ctgttcgcca ttactcgctt cgccaaccac
    18001 ggtgatatct ggtgccatac tgataagctg ttttacgcca gttcgcagca tcgggtgatc
    18061 gtcaatcagc aggatagtag ccggttcctg attactcatg ggtatctcct tggacgtctg
    18121 tgaaagtttt ttcgggaata aaggtgacca ccacttcggt gccacctgat tcacgacggc
    18181 ggacgcggca atcgccccgt aaactttgcg cacggtcgcg cattattatc atgccgtagt
    18241 gattgctgcg gatggcattt tcaggcacgc cgcagccgtt atcctggacg gtcagtttga
    18301 cctgattatc gttttgcgcc actgtcacca cgacctcact cgcttgcgaa tgtttgaggg
    18361 cgttacttaa tgcctcacgg gcaatttgca acagatggat tgcctgatgc gaaggcacca
    18421 gacgaggcgg caattgataa tccagcttca ccgggaagcc aaacctggcg ctgtactctt
    18481 cgcaactcgc ctccagcgcc ggacgtaatc caggctcggt gagctgcaag cggaatgtgg
    18541 tgagcaattc acgcaactgc gcccaggatg cattcagttc gttacggatc tgacttaaca
    18601 gttcgcggct gctttctggc agcgcatcgc cctgcatctg taaacaactc acctgcatct
    18661 tcatgcaaga gagagattgg gcaatagaat catgcagttc gcgcgcaatg gtggcacgct
    18721 cttccatcac gatcaactgt tgctgacgtt cctgatggcg atccagcgca agcgtggcgg
    18781 tgagttgttc aaccagggta tccaccagtt gttgttgatc atgactaaga tgacgcccct
    18841 gcggcagggt tgccagcaaa ataccgtact gcgtatgaga gtcagccagc cgccacttca
    18901 gggttgtgcc gcgatcgcca acggggagta caccgcgcgg gcagagctga cagcctttat
    18961 catcgcaggt catatctggc tggcaggtaa actcctgatg attctcttca tcatcagtgt
    19021 catacacccg cagttcgata tcacgtagca gggttaaatt ctgtaagccg ttgagtacag
    19081 gtgacaggcg ttcacacagc ggggcgcggg aatgcaaacg gcggttggcc tgccataaaa
    19141 aagagaggat ctgattttta tgttccagcc cggcggtttt ctcctgaacc cgctgctcaa
    19201 gtacggcgta actttccgcc agttctgcag acatattgtt caacgcagtt ccaagcatag
    19261 ccatttcgtt gcgcccgctg atgttcgcgc gttgggtaaa atcgcgatgg ctgacggcac
    19321 tcgccattgc caacaactgc cgccacggtt gtagcagtcg cgcccgcaac cagataatag
    19381 tgaacaccag taaaagtgcc ataaataccg ccattacccg atggaccagc accactgtct
    19441 caatgcgcat ttccgtggtg cggtcaaaac cagataccag ttgatcaagc ccggcaacaa
    19501 actggctgac atccgctgac accgtttcgc ggttttgtgc acgcattagc gcagggatca
    19561 gttcattacg ccagtaatct tgtaaaccct gtaattgcgc cagttgtccg tctcgttctg
    19621 ctgctcgggt taactcggcg ctaaatgctg tttgttccat ctctttaatt aagggcttgt
    19681 ctttctcgct taatggcact gctgccaaca gacggtaact ttgcatgcgc agcgatcccg
    19741 ctttgttgat cgcatgggcg ctgccctgaa cgccttgcac cagccagcca gaaactgcca
    19801 tccctgccag tccaatagca gtagaaagca acacaataag cgcaacctga ttaaccaggg
    19861 tgagcggaga gagacaacgt ttaagcatgc aaacctcttc cttcaggctt taaatgagca
    19921 ataaccttaa tgaatgtgac gatacattct ggaatggcag tattctcggc tattggctga
    19981 agtataccca tacccggaaa gagttactcc ttatttgccg cgtggttagt cgctttacat
    20041 cggtaagggt agggatttta cagcaccgtg aaaaatctca taatttttat gaaatcactg
    20101 tactcactat gggtaatgat aaatatcaat gatagataaa gttatcttat cgtttgattt
    20161 acatcaaatt gcctttagct acagacacta aggtggcaga catcgaaacg agtatcagag
    20221 gtgtctatga gtcactcatc cgcccccgaa agggctactg gagctgtcat tacagattgg
    20281 cgaccggaag atcctgcgtt ctggcaacaa cgcggtcaac gtattgccag ccgcaacctg
    20341 tggatttccg ttccctgtct gctgctggcg ttttgcgtat ggatgttgtt cagcgctgtt
    20401 gcggtgaact taccgaaagt cggttttaat tttacgaccg atcagctatt tatgttgacg
    20461 gcgctgcctt cggtttctgg cgcgttatta cgtgttccat actcctttat ggttcctatc
    20521 ttcggtggtc gtcgctggac ggcgttcagc actggtattc tgattattcc ttgcgtctgg
    20581 ctgggttttg ccgtgcagga tacctccacg ccttatagcg tcttcatcat catctctctg
    20641 ctgtgcggct ttgctggcgc gaacttcgca tccagtatgg caaacatcag cttcttcttt
    20701 ccgaaacaga agcagggtgg cgcgctgggt ctgaatggtg gtctgggcaa catgggcgtc
    20761 agcgtcatgc agttggttgc tccgctggtg gtatcactgt cgattttcgc agtatttggt
    20821 agccagggtg tcaaacagcc ggatgggact gagctgtatc tggcgaatgc gtcctgggta
    20881 tgggtgccgt tccttgccat cttcaccatt gcggcgtggt ttggcatgaa cgatcttgct
    20941 acctcgaaag cctccatcaa ggagcagttg ccggtactca aacggggtca tctgtggatt
    21001 atgagcctgc tgtatctggc aaccttcggc tccttcatcg gcttctccgc gggctttgcg
    21061 atgctgtcaa aaacgcagtt cccggatgtt cagattctgc aatacgcttt cttcgggccg
    21121 tttattggtg cgctggcgcg ttctgcaggt ggtgcattat ctgaccgtct gggcggaact
    21181 cgtgtcacac tggtgaactt tatcctgatg gcgattttca gcggcctgct gttcctgacc
    21241 ttaccgactg acggacaggg cggaagcttc atggcgttct tcgcagtctt cctggcgctg
    21301 ttcctgacag ctgggctggg tagtggttcc acttttcaga tgatttccgt gatcttccgt
    21361 aaactgacaa tggatcgcgt gaaagcagaa gggggctctg acgaacgtgc gatgcgtgaa
    21421 gcggcaaccg acacggcggc ggcgctgggt ttcatctctg cgattggcgc gattggtggc
    21481 ttctttatcc cgaaagcgtt cggtagctcg ctggcattaa cgggttcgcc agtcggcgca
    21541 atgaaagtat ttttgatttt ctatatcgcc tgcgtagtga ttacctgggc ggtatatggt
    21601 cggcattcta aaaaataaat cttaatattt gattatcctt tgcgcggcat gatgtcgcgc
    21661 tttttttacc tgtcatttag ttacaacatc cttatgaagt gtggcgtatt tcgccggttt
    21721 tcattaagag ccattaatat gttacccatg gggaatactc cttaataccc atctgcataa
    21781 aaatctcaat agtctaaata actatggatg caaaatatct taatttacag tgatttatgt
    21841 tgaatttatt aattatcctt aaggggtatc ttaggaattt actttatttt tcatccccat
    21901 cactcttgat cgttatcaat tcccacgctg tttcagagcg ttaccttgcc cttaaacatt
    21961 agcaatgtcg atttatcaga gagccgacag gctcccacag gagaaaaccg atgagtaaat
    22021 tcctggaccg gtttcgctac ttcaaacaga agggcgaaac ctttgccgat gggcatggcc
    22081 agcttctcaa taccaaccgt gactgggagg atggatatcg ccagcgttgg cagcatgaca
    22141 aaatcgtccg ctctacccat ggggtaaact gcaccggctc ctgcagctgg aaaatctacg
    22201 ttaaaaacgg tctggtcacc tgggaaaccc agcagactga ctatccgcgt actcgcccgg
    22261 atctgccaaa ccatgaacct cgcggctgcc cgcgcggtgc cagctactcc tggtatcttt
    22321 acagcgccaa ccgtctgaaa tacccgatga tgcgcaaacg cctgatgaaa atgtggcgtg
    22381 aagcgaaggc gctgcatagc gatccggttg aggcatgggc ttctatcatt gaagacgccg
    22441 ataaagcgaa aagctttaag caggcgcgtg gacgcggtgg ttttgttcgt tcttcctggc
    22501 aggaagtgaa cgaactgatc gccgcatcta acgtttacac catcaaaaac tacggcccgg
    22561 accgtgtcgc tggcttctcg ccaattccgg caatgtcgat ggtttcttac gcgtcgggtg
    22621 cgcgttatct ctcgctgctt ggcggtactt gcttaagctt ctacgactgg tactgcgatc
    22681 tgcctcctgc gtctccacaa acctggggcg agcaaactga cgtaccggaa tctgctgact
    22741 ggtacaactc cagctacatc atcgcctggg ggtcaaacgt gccgcagacg cgtactccgg
    22801 atgctcactt ctttactgaa gtgcgttaca aaggcaccaa aaccgttgcc gtcacgccgg
    22861 actacgctga aatcgccaaa ctgtgcgatc tgtggctggc accgaagcag ggcaccgacg
    22921 cagcaatggc gctggcgatg ggtcacgtga tgctgcgtga attccacctc gacaacccaa
    22981 gccagtattt caccgactat gtgcgtcgtt ataccgacat gccaatgctg gtgatgctgg
    23041 aagagcgcga cggttactac gctgcgggtc gtatgctgcg cgctgctgat ctggtggatg
    23101 cgctgggcca ggaaaacaat ccggaatgga aaactgtcgc ctttaatacc aatggagaaa
    23161 tggttgcgcc gaacggttct attggcttcc gctggggcga gaagggcaaa tggaatcttg
    23221 agcagcgcga cggcaaaact ggcgaagaaa ccgagctgca actgagcctg atgggtagcc
    23281 aggatgagat cgctgaagtg ggcttcccat actttggtgg cgacggcacg gaacacttca
    23341 acaaagtgga actggaaaac gtgctgctgc acaagctgcc ggtgaaacgc ctgcaactgg
    23401 ctgatggcag cactgctctg gtgaccaccg tttatgatct gacgttggca aactacggtc
    23461 tggaacgtgg cctgaacgac gttaactgcg caaccagcta tgacgacgtg aaagcctaca
    23521 cccctgcatg ggccgagcag atcactggcg tttctcgcag ccagattatc cgcattgccc
    23581 gtgaatttgc cgataacgct gacaagacgc acggtcgttc gatgattatc gtcggtgcgg
    23641 ggctgaacca ctggtatcac ctcgatatga actatcgtgg tctgatcaac atgctgattt
    23701 tctgcggctg tgtaggtcag agcggtggtg gctgggcgca ctatgtaggt caggaaaaac
    23761 tgcgtccgca aaccggctgg cagccgctgg cgtttgctct tgactggcag cgtccggcgc
    23821 gtcacatgaa cagcacctct tatttctata accactccag ccagtggcgt tatgaaaccg
    23881 tgaccgcaga agagttgttg tcgccgatgg cggataaatc ccgttatacc ggacatctga
    23941 tcgactttaa cgtccgtgca gaacgtatgg gctggctgcc gtctgcgccg cagttaggca
    24001 ctaacccgct gactatcgct cgcgaagcag aaaaagccgg gatgaatccg gtggactata
    24061 cggtgaaatc cctgaaagag ggttccatcc gttttgcggc agaacaacca gaaaacggta
    24121 aaaaccaccc gcgtaacctg ttcatctggc gttctaacct gctcggttct tccggtaaag
    24181 gtcatgagtt tatgctcaag tacctgctgg ggacggagca cggtatccag ggtaaagacc
    24241 tgggtcagca gggcggcgtg aagccggaag aagtggactg gcaggacaac ggtctggaag
    24301 gcaagttgga tctggtggtg acgctggact tccgtctgtc gagcacctgt ctctattccg
    24361 acatcatttt gccgacggcg acctggtacg aaaaagacga catgaatact tcggatatgc
    24421 atccgtttat tcacccgctg tctgctgcgg tcgatccggc ctgggaagcg aaaagcgact
    24481 gggaaatcta caaagccatc gcgaagaaat tctccgaagt gtgcgttggt catctgggca
    24541 aagaaaccga catcgtcacg ctgcctatcc agcacgactc tgccgctgaa ctggcgcaag
    24601 cgctggatgt gaaagactgg aaaaaaggcg aatgtgacct gatcccaggt aaaaccgcac
    24661 cgcacattat ggttgtagag cgcgattatc cggcaactta cgaacgcttt acctctatcg
    24721 gcccgctgat ggagaaaatc ggtaacggcg gtaaagggat tgcctggaac acccagagcg
    24781 agatggatct gctgcgtaag ctcaactaca ccaaagcgga aggtccggcg aaaggccagc
    24841 cgatgctcaa caccgcaatt gatgcagcag agatgatcct gacgctggca ccagaaacca
    24901 acggccaggt ggccgtgaaa gcctgggcgg cattgagtga gtttaccggt cgtgaccata
    24961 cgcatctggc gctgaataaa gaagacgaga agatccgctt ccgtgacatt caggcgcagc
    25021 cgcgcaaaat catctccagc ccgacctggt ctggccttga agatgaacat gtttcttaca
    25081 acgccggtta caccaacgtt cacgagctga tcccgtggcg tacgctctct ggccgtcagc
    25141 agctgtatca ggatcatcag tggatgcgtg atttcggtga aagcctgctg gtttatcgtc
    25201 cgccgatcga cacccgttcg gtgaaagaag tgatgggcca gaaatctaac ggtaatccgg
    25261 aaaaagcgct caacttcctg acaccgcacc agaagtgggg tatccactcc acctacagcg
    25321 acaacctgct gatgctgact ttaggtcgcg gtggtccggt ggtctggttg agtgaagccg
    25381 atgccaaaga gctgggtatc gccgacaacg actggattga agtcttcaac agcaacggtg
    25441 cattgactgc ccgtgcggtt gtcagccagc gtgttccggc agggatgacc atgatgtacc
    25501 acgcgcagga acgtatcgtt aacctgccgg gttcagaaat caccagccag cgcggtggta
    25561 ttcataactc ggtcacccgt atcacgccga aaccgacgca tatgatcggc ggctatgccc
    25621 atctggcata cggctttaac tactacggca ctgtaggttc taaccgcgat gaatttgttg
    25681 tagtgcgtaa gatgaagaac attgactggt tagatggcga aggcaatgac caggtacagg
    25741 agagcgtaaa atgaaaattc gttcacaagt cggcatggtg ctgaatctgg ataagtgcat
    25801 cggttgccac acctgttcag tgacctgtaa aaacgtctgg accagccgtg aaggcgtgga
    25861 atacgcgtgg ttcaacaacg tggaaaccaa gccgggccag ggcttcccga cagactggga
    25921 aaaccaggaa aaatacaaag gcggctggat ccgtaaaatc aacggcaaac tgcaaccgcg
    25981 catgggtaac cgcgccatgc tgctgggtaa aattttcgct aacccgcatc tgccggggat
    26041 cgacgattat tacgagccgt tcgattttga ctatcagaac ctgcataccg cgccggaagg
    26101 cagcaaatcg cagccgattg cccgtccacg ttcgctgatt accggcgaac ggatggcgaa
    26161 aatcgaaaaa gggccaaact gggaagatga cctgggcggc gagtttgaca aactggcgaa
    26221 agacaagaac ttcgacaaca tccagaaggc gatgtatagc cagttcgaaa acaccttcat
    26281 gatgtatttg ccacgcctgt gcgaacactg cctgaacccg gcatgtgtgg cgacctgccc
    26341 gagcggtgcg atttacaagc gtgaagaaga tggcatcgtc ctgatcgacc aggacaaatg
    26401 ccgtggctgg cgtatgtgca tcaccggatg cccgtacaaa aaaatctact tcaactggaa
    26461 gagcggtaag tctgagaagt gcatcttctg ctatccgcgt attgaagccg gtcagccgac
    26521 ggtgtgctct gaaacctgtg tcggtcgtat ccgttatctt ggcgtgctgc tgtacgatgc
    26581 cgacgcgatt gaacgtgcag ccagcaccga gaacgagaaa gatctctacc agcgtcagct
    26641 ggacgtgttc ctcgatccga acgatccgaa ggtcatcgag caggcgatta aagacggtat
    26701 tccgctgagc gttattgaag ccgcacagca gtcgccggtt tataaaatgg cgatggaatg
    26761 gaaactggcg ctgccgctgc atccggaata tcgcacgctg ccgatggtct ggtatgtgcc
    26821 acctctgtcg ccgattcagt ctgcggcaga tgcgggtgag ctgggcagca atggcattct
    26881 gccagacgtc gaaagcctgc gtattccggt acagtatctg gcgaatctgc tgaccgcagg
    26941 tgataccaaa ccggtactgc gcgcgctgaa acgtatgctg gcgatgcgtc attataaacg
    27001 tgctgaaacc gttgacggta aagttgatac ccgtgcgctg gaagaggtcg gtctgaccga
    27061 agcccaggca caggagatgt accgttatct ggcgattgct aactacgaag atcgctttgt
    27121 ggtgccgagc agtcatcgtg aactggcgcg ggaagccttc ccggagaaaa atggctgcgg
    27181 ctttaccttt ggtgatggct gccacggctc agataccaaa ttcaatctgt ttaacagccg
    27241 tcgtatcgat gccatcgacg tgaccagtaa aacggagccg cacccatgat cgaactcgtg
    27301 attgtatcgc gtctccttga atatccggat gctgccttat ggcagcatca acaagagatg
    27361 tttgaggcga ttgccgcgtc gaaaaatctg ccaaaagagg atgcccatgc gctgggcatt
    27421 ttcctgcgcg atttaacgac gatggatccg ctcgatgccc aggcgcagta cagcgaactg
    27481 ttcgaccgtg gccgcgccac gtcactgttg ctatttgaac atgttcacgg tgaatcccgt
    27541 gaccgcggcc aggcgatggt ggatctgctg gcgcagtacg agcagcacgg cttgcagtta
    27601 aacagccgcg aattgcccga ccatctgccg ctgtatctgg agtacctggc gcagctgccg
    27661 caaagcgaag ccgtggaggg tttgaaagat attgcgccga ttctggcatt gctgagcgcg
    27721 cgtctgcaac agcgtgaaag ccgttatgcc gtgctgtttg atctgctgct gaaactggcg
    27781 aataccgcca tcgacagcga caaagtggcg gaaaaaattg ccgatgaagc gcgcgacgat
    27841 acgccgcagg cgctggatgc agtctgggaa gaagagcagg ttaaattctt tgctgaaaaa
    27901 ggctgcggtg attcagcaat cactgctcat cagcgtcgct ttgccggtgc cgtcgcgccg
    27961 caatatctga atatcaccac cggaggacag cactaatgca attcctgaat atgttcttct
    28021 ttgatatcta cccgtacata gccggggcgg tcttcctgat tggtagctgg ctgcgttatg
    28081 actacgggca gtacacctgg cgcgcggcgt ccagccagat gctggatcgc aaagggatga
    28141 acctggcgtc gaacctgttc catatcggta ttctggggat ttttgttggt cacttcttcg
    28201 gtatgctgac gccgcactgg atgtatgaag cctggctgcc gattgaagtg aaacagaaaa
    28261 tggcgatgtt tgctggtggt gccagcggtg tgctgtgtct gattggcggc gtgctgttgc
    28321 tgaagcgtcg tctgttcagc ccacgcgtgc gtgcaaccac taccggagcg gatatcctga
    28381 tcctgtcgct gctcgttatc cagtgcgcgc tgggcctgtt gaccattccg ttctccgccc
    28441 aacatatgga cggtagcgag atgatgaaac tggttggctg ggcgcagtcg gtagtgacct
    28501 tccacggtgg cgcttctcaa caccttgatg gtgtggcgtt tatcttccgt ctgcacctgg
    28561 tgttggggat gacgttattc ctgctgttcc cgttctcgcg tctggtgcac atctggagcg
    28621 taccggtgga gtatctgaca cgtaagtatc agctggtgcg cgctcgtcac taagcgaatt
    28681 ttagtttgca tagaccccgc ttcggcgggg tttttttatg ggcacggtac ggggtgagtt
    28741 gtcggatgcg cttcgcttat ccgacctaca ggggaggata ttgtaggtcc ggtaaacgcg
    28801 gtgccattgg gcagaattag ctcggagtaa caggttttga gtatttacag gaagtgatgg
    28861 tggtggagga aggattactc agcgctgcgc gcttcgccct tcgggtcgtt gcctgcggcc
    28921 acgctctctc gctggcgctc gagtcgaacc ctggtcgaag cttctcatcc ttccccgctt
    28981 gggcagaata tttaatcgcg gattcgtttg agagttcacg gacttttgaa agtgatggtg
    29041 gtgggggaag gattcgaacc ttcgaagtcg atgacggcag atttacagtc tgctcccttt
    29101 ggccgctcgg gaaccccacc acggggtaat gctttttact agcctgctcc cttatcggga
    29161 agcggggcgc atcatatcaa atgacgcgcc gctgtaaagt gttacgttga gaaaaatgaa
    29221 ctggttgcgt aattttcacc cgtaacggat taaaggtaac cagttatttt tgctggcgat
    29281 taaagaataa tcgtccgatt accgtaaaca aagacgcgct gcgccagtac tttgtagagt
    29341 gcgcgactta agacgttttt ctcgacgtca cgacctgcgc gcatcatatc ttcagctgtg
    29401 taggtatgat cgacatgaat aacgtcctgc atgatgattg gaccttcgtc cagattgtca
    29461 ttcacatagt gagcggtagc gccaataatc ttcacgccac gttcataggc ctggtgataa
    29521 ggacgtgcgc cgataaacgc tggcaggaat gaatggtgaa tattgatgat cttattcggg
    29581 aagcgtgcca caaattctgg cgttaatacc cgcatatact tcgccagcac cacgtagtca
    29641 ggctgataag catcaatggc atccgccatc ttttgatcgt gctcgttgcg gcttaaccct
    29701 tcatggctta ccagctcaaa cggaatatca aaacgctcaa ccagagaacg taaagtatcg
    29761 tggttaccaa taactgccgc gatttcgaca tccaggccgc cgtaattggc tttcatcaac
    29821 aaatcgccaa ggcaatgcgc ctctttagtg accagaatca ctatccggcg acgaccggca
    29881 ggattcagct cacgtacgga gccttctggc aatgcgctgt cgagatccgc cagcagggtg
    29941 gaatcattaa aaattccttc cagttccgtg cgcataaaaa agcgcccggt acggtgatca
    30001 acaaattcat tgttctgtac gatatttaac tcgtgcttgt agcaaatatt ggtaatacgt
    30061 gcgatcagac ctttttggtc cggacaaata gtacgcagaa ctttacgttg gagtgaatgc
    30121 attgctggaa aaaccttgtt gagagtgttt gctaaaccgt accgtcaacc attattggcc
    30181 gcagcacttt ttaaattttt tacctgaacc acaagggcag ggatcgttgc ggccaaactg
    30241 cggacgtgta ccgtcaatat agtaccattg accgttttct ttcaaaaatc gcgaacgttc
    30301 aattattgca cctgttttgc taccttcggt aaatcgcgca acaaaactga caaatccgat
    30361 attgtcgacg tcctgccaac aatgttcaaa tacggtcaat cccaaccatt cagtatgcgc
    30421 aaatccggca atcaattcgg cacgtaacgt cgccgctcca caagatggat gccaggtctt
    30481 tattaaataa tctgcgtctt tcatcacaaa agcgcagtaa cgcgaacgca tgagatgttc
    30541 tggatcaggt gcaacctttt caccagacac ataagggtgg caacataggc tatactcgac
    30601 agcactacca cagggacaaa gctgagacac aaataatctc cctggaaaca ataacggcgt
    30661 attaaccgcc tgagtagcac tatgttaacc gagcagtagc gatgttgcta cgattgcatt
    30721 ccaggggaat cttgcgggaa taatgagaaa gataaaaata gggctggcgc tgggatctgg
    30781 cgcggcgaga ggttggtcgc atattggcgt tattaatgcg ctaaaaaaag tgggtattga
    30841 aattgatatc gttgcaggat gttcaattgg ttcgctggtg ggcgctgcct atgcatgcga
    30901 tcgactatct gcgctggaag attgggtgac ctctttcagt tattgggatg ttttacgcct
    30961 gatggatctc tcctggcagc gcggtgggtt actgcgcggc gagcgtgtct tcaatcaata
    31021 tcgcgaaata atgccggaaa cagagatcga aaattgttcc cgtcgcttcg cggctgttgc
    31081 caccaattta agtactgggc gcgaattatg gtttactgaa ggcgatctcc atcttgctat
    31141 tcgtgcatca tgcagtattc caggactaat ggcacccgtt gcgcataacg gctactggct
    31201 ggttgatggc gctgtagtta acccaattcc tatttccctc acgcgtgcat tgggggctga
    31261 tattgtgata gcggtcgacc tgcagcacga tgctcatttg atgcaacaag atttgctctc
    31321 ctttaatgtc agtgaagaaa atagcgagaa tggtgattct ctgccgtggc atgcgcgtct
    31381 gaaagaaagg ttaggcagca taacgacacg tcgggcggtg acagcgccaa cggcaacaga
    31441 gattatgacc acttctatcc aggtgctgga gaaccgcctt aaaaggaacc gcatggcagg
    31501 tgatccgccc gatattctga ttcaacctgt ttgcccgcaa atatctacgc ttgatttcca
    31561 tcgcgcgcac gctgccattg cggccgggca gctggcagtg gaaaagaaaa tggacgaact
    31621 tttgccgttg gtacgcacca acatttgacc agaattttta tctacactta agttaattct
    31681 gacaggcgca ggtggcaata gcatgccact attgagtaaa gccagtcagg ggagagaaca
    31741 tgacgcagcc attggtcgga aaacagattc tcattgttga agatgagcag gtatttcgct
    31801 cgcttctgga ttcgtggttt tcctcattgg gagcgacaac ggtactggcg gctgatgggg
    31861 tggatgccct tgagttgctg ggaggtttca ctccagacct gatgatatgt gatatcgcga
    31921 tgccacgaat gaacgggctt aaactgctgg agcatatacg taacagaggc gaccagaccc
    31981 cagttctggt gatatctgcc actgaaaata tggcagatat tgccaaagcg ttacgtctgg
    32041 gcgttgaaga tgttttgctg aaaccagtta aagatctgaa tcgcttgcgc gagatggttt
    32101 ttgcctgtct ctatcccagc atgtttaatt cccgcgttga ggaagaggaa aggctttttc
    32161 gcgactggga tgcaatggtt gataaccctg ccgcagcggc gaaattatta caggaattgc
    32221 aaccgccggt tcagcaggtg atttcccatt gccgggttaa ttatcgtcaa ttggttgccg
    32281 cggacaaacc cggcctggtg cttgatattg ccgcactttc ggaaaacgat ctggcatttt
    32341 actgccttga tgtcacccga gctggacata atggcgtact tgctgccttg ttattacgcg
    32401 cattgtttaa cggattatta caggaacagc ttgcacacca aaatcaacgt ttgccagagt
    32461 tgggcgcgtt attgaagcag gtaaaccatt tactccgtca ggccaatctg ccggggcagt
    32521 ttccgctatt agttggctat tatcatcgcg aactgaaaaa tctcattctg gtttctgcgg
    32581 gtctgaatgc gacgttaaat accggcgaac accaggtgca aatcagtaat ggtgttccgt
    32641 taggcacttt aggtaacgct tatttgaatc aattgagcca gcgatgcgat gcctggcaat
    32701 gccaaatatg gggaaccggt ggtcggcttc gcttgatgtt gtctgcagaa tgagcaaacg
    32761 ataacgcggg ctaaatttgc attacctgct aatgtcggct ggtggtacta tcgtcgccat
    32821 tcgtataagt aattgtctta attatgctaa ctcgcctcct tttcagaact tagccccttc
    32881 ggggtgctga tatactggga tgcgatacag aaatatgaac acgttcaaaa cacgaacagt
    32941 ccaggagaat ttaaatggct gccattaata cgaaagtcaa aaaagccgtt atccccgttg
    33001 cgggattagg aaccaggatg ttgccggcga cgaaagccat cccgaaagag atgctgccac
    33061 ttgtcgataa gccattaatt caatacgtcg tgaatgaatg tattgcggct ggcattactg
    33121 aaattgtgct ggttactcac tcatctaaaa actctattga aaaccacttt gataccagtt
    33181 ttgaactgga agcaatgctg gaaaaacgtg taaaacgtca actgcttgat gaagtgcagt
    33241 ctatttgtcc gccgcacgtg actattatgc aagttcgtca ggggctggcg aaaggcctgg
    33301 gacatgcggt attgtgtgct cacccggtag tgggtgatga acctgtggct gttattttgc
    33361 ctgacgttat tctggatgaa tatgaatccg atttgtcaca ggataacctg gcagagatga
    33421 tccgccgctt tgatgaaacg ggtcatagcc agatcatggt tgaaccggtt gctgatgtta
    33481 ccgcatatgg cgttgtggat tgcaaaggcg ttgaattagc gccgggtgaa agcgtaccga
    33541 tggttggtgt ggttgaaaaa ccaaaagcgg atgttgcgcc gtctaatctc gctattgtgg
    33601 gtcgttacgt acttagcgcg gatatttggc cgttgctggc aaaaacccct ccgggagctg
    33661 gtgatgaaat tcagctcacc gacgcaattg atatgctgat cgaaaaagaa acggtggaag
    33721 cctatcatat gaaagggaag agccatgact gtggtaataa attaggttac atgcaggcct
    33781 tcgttgaata cggtattcgt cataacaccc ttggtacgga atttaaagcc tggcttgaag
    33841 aagagatggg cattaagaag taacatccgt atcggtgtta tccacgaaac ggcgttgagc
    33901 aatcgacgcc gtttttttat agcttattct tattaaattg tcttaaaccg gacaataaaa
    33961 aatcccgccg atggcgggat ttttaagcaa gtgcaatcta caaaagatta ttgcttgatc
    34021 aggaaatcgt cgagggattt accttgctca tccattgctt ttttgattac agctggagta
    34081 cggccctggc cagtccaggt tttagtttcg ccgttttcgt caacgtagct atattttgcc
    34141 ggacgctgag cacgtttagc tttggtgcca gatttaacgg cagcaaggct attcagcagt
    34201 tcgttcgggt caataccgtc agcgatcagc atttcgcgat attgctgcag tttacgagtg
    34261 cgctcttcaa cttcagcagc agccgcgctt tcttcttcgc gacgttcgtt aacaacaact
    34321 tctaattttt ccagcatttc ttccagcgtt tcaagtgtac attctcttgc ctgcgcacga
    34381 agagtacgga tgttgttcag aattttaagt gcttcgctca ttgtagtaat ctcaaactta
    34441 tattggggtg gtttgttgag gtaataatag agccttaaat tcagttgtgc aatagccagg
    34501 aatgtaagga attcaaaatt gttctttatt ttgtgccgcc aataaatatc ttttcataaa
    34561 attagccaga aaagacgcgg catatagccc tatttgcgcc gatgatttcg cagcacgtga
    34621 ggttaaaact tcctgattca tgtcacattt tatggggaga ttatcgtagg ctgacgacct
    34681 ttcagtcttc tgtattagtc gtgtttacaa gaattcccta ttaagcgaat gatgaaaagt
    34741 agaacagtcg caataagagc agggacttag tattgcacta tctcctggag gtcaacagag
    34801 ggctattact tgcgcaacag gttaaagatt gtgaatagtt accagcagtc atttacccgc
    34861 ttataacaag cgaggcagtt gtaatgatag ctcagaagga ttatgcaagg cttcgtaagg
    34921 gagaacgcat ataccacttc tgtgcatact gttgagctga aaaactgacg aattatgata
    34981 aactccagcc aactttaatt catatcattg agggcctgtg gctgatggca cagctatatt
    35041 tctactattc cgcaatgaat gcgggtaagt ctacagcatt gttgcaatct tcatacaatt
    35101 accaggaacg cggcatgcgc actgtcgtat atacggcaga aattgatgat cgctttggtg
    35161 ccgggaaagt cagctcgcgt ataggtttgt catcgcctgc aaaattattt aaccaaaatt
    35221 catcattatt tgatgagatt cgtgcggaac atgaacagca ggcaattcat tgcgtactgg
    35281 ttgatgaatg tcagttttta accagacaac aagtatatga attatcggag gttgtcgatc
    35341 aactcgatat acccgtactt tgttatggtt tacgtaccga ttttcgaggt gaattattta
    35401 ttggcagcca atacttactg gcatggtccg acaaactggt tgaattaaaa accatctgtt
    35461 tttgtggccg caaagcaagc atggtgctgc gtcttgatca agcaggcaga ccttataacg
    35521 aaggtgagca ggtggttatt ggtggtaatg aacgctacgt ttctgtatgc cgtaaacact
    35581 ataaagaagc gttagaagtc ggctcattaa cggctattca ggaaaggcat cgccacgatt
    35641 aataagaatt tctttactga cagggtgagc agggcgcttt tatcctgtca gttcgtttta
    35701 ctcactttat ccggtgtata tacccttctc gacagttctt taacgccgct atacccctca
    35761 cagagcgctt ttacagtgac gttgacttgt gacctgtaaa gtacaatatc gctgtgtgta
    35821 ggcgttatac atcgccgtaa atatgatgca ggcaaatgct gtcggtttat gggaaagttg
    35881 ctttgggtaa aaaaaacggc cccagaaggg ataatgccag tcagttaagc aactgactgg
    35941 ctctttttcg gggctgtggg gtatttccag ggcctctcct ttaccactct cgggaaggc
//
LOCUS       DAZKJR010000035        35748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKJR010000035 DAZKJR010000000
VERSION     DAZKJR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.035"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(145..3294)
                     /gene="acrB"
                     /locus_tag="ACHOWS_001771"
     CDS             complement(145..3294)
                     /gene="acrB"
                     /locus_tag="ACHOWS_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFQ0047352.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3317..4510)
                     /gene="acrA"
                     /locus_tag="ACHOWS_001772"
     CDS             complement(3317..4510)
                     /gene="acrA"
                     /locus_tag="ACHOWS_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFQ0047353.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATTLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4652..5299
                     /gene="acrR"
                     /locus_tag="ACHOWS_001773"
     CDS             4652..5299
                     /gene="acrR"
                     /locus_tag="ACHOWS_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFQ0047354.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5427..8789
                     /gene="mscK"
                     /locus_tag="ACHOWS_001774"
     CDS             5427..8789
                     /gene="mscK"
                     /locus_tag="ACHOWS_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFQ0047355.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLRDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8850..8948
                     /locus_tag="ACHOWS_001775"
                     /pseudo
     CDS             8850..8948
                     /locus_tag="ACHOWS_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9001..9162)
                     /gene="rsmS"
                     /locus_tag="ACHOWS_001776"
     CDS             complement(9001..9162)
                     /gene="rsmS"
                     /locus_tag="ACHOWS_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFQ0047356.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9176..9703)
                     /gene="priC"
                     /locus_tag="ACHOWS_001777"
     CDS             complement(9176..9703)
                     /gene="priC"
                     /locus_tag="ACHOWS_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFQ0047357.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9773..10150
                     /gene="ybaN"
                     /locus_tag="ACHOWS_001778"
     CDS             9773..10150
                     /gene="ybaN"
                     /locus_tag="ACHOWS_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFQ0047358.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10303..10854
                     /gene="apt"
                     /locus_tag="ACHOWS_001779"
     CDS             10303..10854
                     /gene="apt"
                     /locus_tag="ACHOWS_001779"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0047359.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10983..12914
                     /gene="dnaX"
                     /locus_tag="ACHOWS_001780"
     CDS             10983..12914
                     /gene="dnaX"
                     /locus_tag="ACHOWS_001780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0047360.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNSVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12247..12311
                     /gene="dnaX"
                     /locus_tag="ACHOWS_001780"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12967..13296
                     /gene="ybaB"
                     /locus_tag="ACHOWS_001781"
     CDS             12967..13296
                     /gene="ybaB"
                     /locus_tag="ACHOWS_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0047361.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13296..13901
                     /gene="recR"
                     /locus_tag="ACHOWS_001782"
     CDS             13296..13901
                     /gene="recR"
                     /locus_tag="ACHOWS_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0047362.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            14011..15885
                     /gene="htpG"
                     /locus_tag="ACHOWS_001783"
     CDS             14011..15885
                     /gene="htpG"
                     /locus_tag="ACHOWS_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFQ0047363.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16066..16710
                     /gene="adk"
                     /locus_tag="ACHOWS_001784"
     CDS             16066..16710
                     /gene="adk"
                     /locus_tag="ACHOWS_001784"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0047364.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            16842..17804
                     /gene="hemH"
                     /locus_tag="ACHOWS_001785"
     CDS             16842..17804
                     /gene="hemH"
                     /locus_tag="ACHOWS_001785"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFQ0047365.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17801..18760)
                     /gene="aes"
                     /locus_tag="ACHOWS_001786"
     CDS             complement(17801..18760)
                     /gene="aes"
                     /locus_tag="ACHOWS_001786"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFQ0047366.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FTGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18912..20216
                     /gene="gsk"
                     /locus_tag="ACHOWS_001787"
     CDS             18912..20216
                     /gene="gsk"
                     /locus_tag="ACHOWS_001787"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFQ0047367.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20300..21976)
                     /gene="ybaL"
                     /locus_tag="ACHOWS_001788"
     CDS             complement(20300..21976)
                     /gene="ybaL"
                     /locus_tag="ACHOWS_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFQ0047368.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22214..23434)
                     /gene="fsr"
                     /locus_tag="ACHOWS_001789"
     CDS             complement(22214..23434)
                     /gene="fsr"
                     /locus_tag="ACHOWS_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFQ0047369.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23652..25304
                     /gene="ushA"
                     /locus_tag="ACHOWS_001790"
     CDS             23652..25304
                     /gene="ushA"
                     /locus_tag="ACHOWS_001790"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0047370.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLIMAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNNLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKAGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25341..25820)
                     /gene="ybaK"
                     /locus_tag="ACHOWS_001791"
     CDS             complement(25341..25820)
                     /gene="ybaK"
                     /locus_tag="ACHOWS_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFQ0047371.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26024..26818)
                     /gene="ybaP"
                     /locus_tag="ACHOWS_001792"
     CDS             complement(26024..26818)
                     /gene="ybaP"
                     /locus_tag="ACHOWS_001792"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFQ0047372.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEKRISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26951..27253
                     /locus_tag="ACHOWS_001793"
     CDS             26951..27253
                     /locus_tag="ACHOWS_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0047373.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            27289..27630
                     /gene="ybaQ"
                     /locus_tag="ACHOWS_001794"
     CDS             27289..27630
                     /gene="ybaQ"
                     /locus_tag="ACHOWS_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFQ0047374.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27688..30192)
                     /gene="copA"
                     /locus_tag="ACHOWS_001795"
     CDS             complement(27688..30192)
                     /gene="copA"
                     /locus_tag="ACHOWS_001795"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFQ0047375.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTNEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30455..31387
                     /gene="glsA"
                     /locus_tag="ACHOWS_001796"
     CDS             30455..31387
                     /gene="glsA"
                     /locus_tag="ACHOWS_001796"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0047376.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31390..32682
                     /gene="ybaT"
                     /locus_tag="ACHOWS_001797"
     CDS             31390..32682
                     /gene="ybaT"
                     /locus_tag="ACHOWS_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0047377.1"
                     /translation="MMNTEGNNDNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            32713..32810
                     /locus_tag="ACHOWS_001798"
                     /pseudo
     CDS             32713..32810
                     /locus_tag="ACHOWS_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32807..33214
                     /gene="cueR"
                     /locus_tag="ACHOWS_001799"
     CDS             32807..33214
                     /gene="cueR"
                     /locus_tag="ACHOWS_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFQ0047378.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33215..33945)
                     /locus_tag="ACHOWS_001800"
                     /pseudo
     CDS             complement(33215..33945)
                     /locus_tag="ACHOWS_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34017..34223)
                     /locus_tag="ACHOWS_001801"
     CDS             complement(34017..34223)
                     /locus_tag="ACHOWS_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047379.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(34220..34642)
                     /locus_tag="ACHOWS_001802"
     CDS             complement(34220..34642)
                     /locus_tag="ACHOWS_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047380.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(34728..35744)
                     /locus_tag="ACHOWS_001803"
     CDS             complement(34728..35744)
                     /locus_tag="ACHOWS_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFQ0047381.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     TQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLSNTQKRVADNSQQINTLNNHYSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT         8690 a         9325 c         9369 g         8364 t
ORIGIN      
        1 caatcagtat tttttatgaa ttttatacgt gtttaatact ggttttcgta tgagatcctg
       61 agttggtggt tcaatgactc cttaatgttc gtcggttatg cataaaaaag gccgcttgcg
      121 cggccttagt gattacacgt tgtatcaatg atgatcgaca gtatggctgt gctcgatatc
      181 ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa cgaagaagat
      241 tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg cgttctgcgc
      301 gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa acgccaacga
      361 ggtcatcagg attgggcgta aacgcatccg caccgcatca agcgtcgctt caatcagacc
      421 tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg cgttcttcgc
      481 cgacaaacca atggttgtga gcaggcctac ctggaagtaa acgtcattgg tcaggccacg
      541 gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca taacggagaa
      601 cggaatcgac cagctctcgt acagcgcagc cagacacagg aacacgacaa tcaacgaaat
      661 cgcgtacagt gaaggcgcct ggttgccgga gagacgttcc tgataggaca ttcccgtcca
      721 gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct ccattgcttc
      781 accggtactt ttacccggtg ccgcctggcc taagatttcc atagatggca ggccgttgta
      841 acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg agaacggcac
      901 catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca gcatacggta
      961 tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa agtcgttcac
     1021 atagctaccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag aaacacccag
     1081 cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt ccagaccgtt
     1141 tggacgtacg ctggtcaaca tatcagggtg cttcgctgct tctgcaagca actggttacg
     1201 cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa agtcaaagcc
     1261 ggttgcggta cccagttcca cgattgcggg caggttaaag gcgaaaacca tcgcatcttt
     1321 gatttgcgag aaagcacgtg ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc
     1381 cggacgatcg gcccagtcct tcaaggaaac gaacgcaata cctgtattct gaccacgtcc
     1441 cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt ctttggtcag
     1501 atagtaatgc gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg cacctgctgg
     1561 cagctgaacc atggtcataa atacgccctg gtcttcatct ggcaagaagg agcttggcag
     1621 acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacca ggtaacgccc
     1681 tgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat
     1741 gcggttaaac cagccgaaga agcctttttt accttccccg tgatcgcctt tggcaatcgg
     1801 tttcagcatg gtggcacaaa gagccggagt caggatcaac gccaccagta ccgacagcgc
     1861 cattgctgaa acaatggtaa tagagaactg acgatagata gcaccagtag aaccgccaaa
     1921 gaaggccatc ggtacgaata ccgccgacag taccatcgcg ataccgacca gagcgccctg
     1981 aatctgcccc atcgacttac gggtagcttc ttttggcggc aaaccttctt ccgccataac
     2041 acgctcaacg ttttctacca caacgatggc gtcatccacc aacaggccga tggcgagcac
     2101 catcccgaac attgttagcg tgtttatcga gaagccaaag gcggcaagga cggcaaaggt
     2161 ccccagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga agttctgcag
     2221 gaacagatac ataaccagga acacgaggat gatcgcttcg actagcgttt taaccacttc
     2281 gtgaatcgag attttcacga acggcgtggt gtcgtacggg taaacaattt tcagacccga
     2341 cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg tatccagcgc
     2401 gtttgcaccg gtcgccagct tgatccccag accggaagcc ggttggccgt taaactctgc
     2461 gatgatgtcg tagttctcac cacccagctc aattttcgcc acatcacgca gcagcacgcg
     2521 ggaaccatcc tgattcactt tcagcaggat tttgccgaac tcttcagtag aggtcagacg
     2581 cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg taccaccgag
     2641 ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat caaccggcgt
     2701 tagctggaat ttgttcagtt cattcgggtt catccagata cgcatcgcat actgtgaacc
     2761 gaacaactga acgtcaccca cgcccgacgt acggctgatg gcatctttca tattcgctgc
     2821 cacgtagtca gagatatcct cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac
     2881 catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgta cttcttgcgg
     2941 cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga tatccgcatc
     3001 agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac tgttagagga
     3061 catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg tcaccgtgtc
     3121 ctgcactgtt ttcgcatcag cgccagggta ggaggcggag atcgttactg ccggcggtgc
     3181 aatcgtagga tattgcgcca ccggcagttt gaggatcgcc agcccccctg ccaacatgat
     3241 gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag gcatgtctta
     3301 acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt gcggcttgct
     3361 ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc actttctgca
     3421 gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc
     3481 cgatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg ccaaccacca
     3541 gtacggtggc atcgccacgc ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt
     3601 ttggattaag cccttcttcc agacgtgccc gcacgaacat acccggcagc agagtgtgat
     3661 ccgggttcgg gaagatagcg cgtagggtga tagacccggt ggtctgatca acggtaacgt
     3721 cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg atcagcgaca
     3781 ctttggcttt gccgttttct tgtttcaacg tgccattcgc cagttcctgt ttcaggcgca
     3841 ggaagtcgtt gctggactgg gtcacatcaa cgtagatcgg atcaagttgc tgcacggttg
     3901 ccagcgtagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc gacttaccaa
     3961 tgcgaccgct aatcggcgag gtgactttgg tgtaagccag attgatccgc gcagtttcaa
     4021 cggcagcttt cgccgcagtg accgcagcat tcgcctgttg cgcatcagcc agagcctgat
     4081 cgtactcttg tttactgatg tactgagtac cgagcagttt ctgataacga ttcaccgtca
     4141 attgcgcgat attggctgca gcctgggctt tcgccaaatc acctttcgca ctgtcgtatg
     4201 ccgcctgata ggtcgcagga tcaatctgat agagagagac acctgcttcg atgtcgctac
     4261 cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact tctgcgatcc
     4321 ggtaggcact ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca gttttgactg
     4381 ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg tcgtcacatc
     4441 ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta aaccctctgt
     4501 ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa
     4561 taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc acaaatgtat
     4621 gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac aagaagcgca
     4681 agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc agggggtatc
     4741 atccacctcg ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg caatctactg
     4801 gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag aatccaatat
     4861 tggtgaactc gagcttgagt atcaggcaaa attccctggc gatccactct cagtattaag
     4921 agagatatta attcatgttc ttgaatccac ggtgacagaa gaacggcgtc gattattgat
     4981 ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca
     5041 acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac attgtattga
     5101 agcgaaaatg ttgcctgcgg atttaatgac gcgtcgggca gcaattatta tgcgcggcta
     5161 tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc ttaaaaaaga
     5221 agcccgcgat tacgtcgcca tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa
     5281 tcctgccact aacgaataac cttgaatctg actccaggat ttttcctgga cattttcgtc
     5341 gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc
     5401 ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa cgatcacggc
     5461 attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag aacacggcgt
     5521 ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag gcacaacttg
     5581 actcactgaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag caggatctga
     5641 cagatacatt agctaccctc gataaaatcg atcgcgtaaa agaagagaca gttcagctac
     5701 ggcaaaaggt tgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg ttaactgcac
     5761 ttagcgatgt cgataatgac gaagaaactc gcaaaattct gagtacgctt tcgttgcgcc
     5821 agctggaaac tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa aacgatctgg
     5881 cgtcttataa cagccagctg gtttcgttac agacgcagcc cgaacgcgtg caaaatgcga
     5941 tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg
     6001 gcgagactgc cttacgtccc agccagaaag tgttaatgca ggctcagcag gctttgctga
     6061 atgccgagat tgaccagcag cgtaaaagcc tggaagggaa cacagtctta caggatacct
     6121 tgcaaaagca acgggattac gtgacggcga acagcgcccg tctggagcac cagttgcagc
     6181 tgttgcaaga agcagtaaac agcaagcgct tgactttaac cgaaaaaacg gcgcaggaag
     6241 ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaag caggaactgg
     6301 aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt aatcagttga
     6361 tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg gaacgcaata
     6421 ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc ctttaccagc
     6481 aacaacaaac gctgccctcg gcggatgaac tggaaaacat gaccaaccgc atcgcggatt
     6541 tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag agcgatgcgt
     6601 tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt
     6661 tattgcaagt ggttgatatg cgtcgcgaat tgctggatca gcttaacaaa cagttgggta
     6721 accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg agtgtgtcga
     6781 aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaatagtaac cgtccaatgg
     6841 actgggactg gatcaaagcg ttcccgcaaa gcctgagaga tgaatttaag tcgatgaaaa
     6901 tcacggtgaa ctgggaaaaa gcctggcctg ccgtttttat cgctttcctc gctggtttgc
     6961 cgctgctgtt gattgccggg ctgatccact ggcgtctggg ctggctgaaa gcgtatcaac
     7021 aaaaactggc ttccgctgtg ggttccctgc gtaacgacag ccagctcaac acaccaaaag
     7081 cgatccttat cgacctgatc cgtgcgctgc cggtgtgcct gattattctc gcggttggcc
     7141 tgattctgtt gaccatgcag ctcaacatca gcgaactgct atggtcgttc agtaaaaaac
     7201 tggcgatttt ctggctggtg tttggcctgt gctggaaagt gttggagaaa aacggcgttg
     7261 ccgtgcgtca ctttggtatg ccggaacagc agaccagcca ctggcgtcgg caaattgtcc
     7321 gcatcagtct cgcgttgctg cctatccatt tctggtctgt ggtggcagaa ctttccccgc
     7381 tgcatctgat ggatgatgtg ctggggcagg cgatgatttt cttcaacttg ctgctgattg
     7441 ccttcctggt gtggccgatg tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc
     7501 gactggtcac cattaccgta ctgtcgatta tcccgattgc gttgatggtg ctgaccgcca
     7561 cgggttattt ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc gtttatttgg
     7621 tgatcatctg gaacctgctg taccagacgg tattgcgcgg cttaagcgta gcggcgcggc
     7681 gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag
     7741 gtgctgaacc gccggaagaa cccaccattg cactggagca ggttaaccag cagacgctgc
     7801 gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca atttggtccg
     7861 atttgatcac cgtgttcagc tatctggaca gcatcacgct ctggcattac aacggcactg
     7921 aagctggtgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt gcgattatcg
     7981 cctcaatggt ggcctgggcg ttgattcgca acctgcctgg tttgctggaa gtgttggtgc
     8041 tctcgcgcct gaacatgcgc cagggcgcgt cgtatgccat tactaccatc cttaactaca
     8101 tcattattgc cgttggtgcg atgacggtgt tcggatcgct gggcgtctcg tgggataaac
     8161 tccagtggct ggcggcagca ttatccgtag gtcttggttt tggtttacaa gagatcttcg
     8221 gtaacttcgt ttccggtttg atcattctgt tcgaacgtcc ggtgcgcata ggcgacacgg
     8281 taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg acaacgatta
     8341 ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga
     8401 tcaactggtc gttgactgac acgactacgc gtctggtgat ccgtctcggc gtagcttatg
     8461 gctccgatct ggaaaaagtg cgtaaagtgt tgctgaaggc ggcgactgag cacccaaggg
     8521 tgatgcacga accaatgccg gaagtcttct ttacggcatt tggtgccagc acgttggatc
     8581 atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag ccgtactgtc gatgagctga
     8641 accgtactat cgatcagttg tgccgtgaaa acgacatcaa cattgccttt aaccagcttg
     8701 aagtgcatct gcacaacgag aagggcgatg aggtgacgga agtcaaacgc gactacaaag
     8761 gcgacgatcc gacgccagcg gtagggtaat aaccaaaggg ggcgacattt agttgccccg
     8821 agattactaa caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc
     8881 tacgaaaccg caagaattca atatattgca ggagcggtgt aggcctgata agcgtagcgc
     8941 atcaggcagt tttgcgtttg cccacaatct taggggacat ttagcggccc catttatttc
     9001 tcacttttcc gcctcatcat cgcgcgttaa tttcttttca taatcgcgtt ttacaatatc
     9061 cagcgcgcgc agcacggtgc gggctgggat ctgattttct tccagtagca caatcaaatc
     9121 gacggccagt ttgacatcgt caggggcatt ttccagtgac atactctctc cattgctagc
     9181 gggttaaacg cgctaacctg ttttcgattt tttccagcgc atggcggcag cgtgccaggc
     9241 gcgcttcata ggcttccact tcacgatgca gcgtctgctg ttccacgaga tcggtcgctc
     9301 gtgccagacg ggctctacgt tcggcaacca tctcacgtag ccgccgctca aaatcctgat
     9361 gctgaatacg tttacgctgc cagcgggcaa ttttcggtgg tgcgctatcc cactcgcgta
     9421 gcgaccaggc tgtggcttca cgcgctatag cctccagttg cgccgccaga tgttctgcca
     9481 gccaggcgac ttgcggcagc tgttgctgct caaccgcatg acgaagcgca gccagattat
     9541 cgcccgcctc atcgagacaa gcctgcagtg ttgtcgcacg agtctgaaaa agatgcctgt
     9601 caaaacgggc gcttagcgtg gcaaattgcg ctaccggggc acaacgctga cgcagcgtag
     9661 cgagctgtcc ttccagtttt tccagcagca gggcggtttt cacgataaga cctcaatgaa
     9721 aatgataatt gttatgctaa agtagcaact ctgtaagctg acaacactca atatgcaacg
     9781 aatcatttta atcatcattg gctggctggc ggtagtgctg ggtacgctgg gcgtggtatt
     9841 accggtatta ccgacgacgc cgtttatcct gctggcggcc tggtgctttg cccgttcttc
     9901 cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt ggcagctatc tacgtttctg
     9961 gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc ttattttgct
    10021 cacgtttgcc atttctctgt ggttcgtcca gatgccatgg gtgcgcatca tgttgctggt
    10081 aattctcgcc tgtttgcttt tttatatgtg gcgaattccg gtgattgatg aaaagcaaga
    10141 aaagcactga agcacaacaa tcgcagttgc aattattgcg tacagccagt acattctggc
    10201 gttttcgagc acaggcgcag gcggtcaaag gttaaacaac tgttactttt gatacgttta
    10261 aaacgcgccg tgagtaccac cgtaacaagc aggcatacac ttatgaccgc gactgcacag
    10321 cagcttgagt atctcaaaaa tagcatcaaa agcattcagg actacccaaa acccggcatt
    10381 cttttccgtg atgtcaccag cttactggaa gacccgaaag cttacgctct cagcatcgac
    10441 ttgctggttg agcgttacaa aaatgcgggc attaccaaag ttgtcggcac cgaagcgcgt
    10501 ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt accggtccgt
    10561 aaaccgggca aactgccgcg tgaaaccatc agtgaaactt acgacctgga atacggcacc
    10621 gatcagctgg aaatccacgt tgatgccatc aaaccgggcg acaaagttct ggtggtggac
    10681 gatctgctgg caaccggcgg cactatcgaa gcgaccgtta aactgatccg tcgtctgggt
    10741 ggtgaagtgg ctgacgctgc gttcattatc aacctgttcg atctcggcgg cgaacagcgt
    10801 ctcgaaaaac agggcattac cagctacagc cttgtcccgt tcccgggcca ttaattatcg
    10861 ccagtcttgt gctgcccacg ctacggacag cacaagatgt gcattcagcc tcgccgttct
    10921 gacggggctg tgttagcatt accccttcgt gaatccacct tccagcgttt cagagcctgc
    10981 caatgagtta tcaggtctta gcccgaaaat ggcgcccaca aacctttgct gacgtcgtcg
    11041 gccaggaaca tgtgctgacc gcactggcga acggcttgtc gttagggcgt attcatcatg
    11101 cttatctttt ttccggtacc cgtggcgtcg gaaaaacctc tatcgcccga ctgctggcga
    11161 aggggctaaa ctgcgaaacc ggcattaccg cgacgccgtg cggcgtgtgc gataactgtc
    11221 gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc tcgcgcacta
    11281 aagtcgaaga tacccgcgac ctgctggata acgttcagta cgctccggcg cgtggtcgtt
    11341 tcaaagttta tctcatcgac gaagtgcata tgctgtcgcg tcacagcttt aacgcactgt
    11401 taaaaaccct tgaagagccg ccagagcacg ttaagttttt gctggcgacg accgatccgc
    11461 agaaattgcc ggtgacgatt ttgtcacgct gtctgcaatt tcatctcaag gcgctggatg
    11521 tcgagcaaat tcgccatcag cttgagcaca tcctcaacga agaacatatc gctcacgagc
    11581 cgcgggcgct gcaattgttg gcacgcgccg ctgaaggcag cctgcgagat gctttaagtc
    11641 tgaccgacca ggcgattgcc agcggtgacg gccaggtttc aacccaggcg gtcagtgcga
    11701 tgctgggtac gcttgacgac gatcaggcgt tatcgctggt tgaagcgatg gtcgaggcca
    11761 acggcgagcg cgtaatggcg ctaattaatg aagctgctgc ccgtggtatc gagtgggaag
    11821 cgttgctggt ggaaatgctc ggtctgttgc atcgtattgc gatggtacaa ctttcgcctg
    11881 ctgctcttgg caacgacatg gccgccattg agctgcggat gcgtgaactg gcgcgcacca
    11941 taccgccgac ggatattcag ctttactatc agacgctgtt gattggtcgc aaagaattac
    12001 cgtatgcgcc agaccgccgt atgggtgttg agatgacgct gctgcgcgcg ctggcgttcc
    12061 atccgcgtat gcctctgcct gagccagaag tgccacgcca gtcctttgcg cccgttgcgc
    12121 caacggcagt aatgacgcca acacaggtgc cgccgcaacc gcaatcagcg ccgcagcagg
    12181 caccgactgt accgctcccg gaaaccacca gccaggtgct ggcggcgcgc cagcagttgc
    12241 agcgcgtgca gggagcaacc aaagcaaaaa agagtgaacc ggcagccgct acccgcgcgc
    12301 ggccggtgaa taacgctgcg ctggaaagac tggcttcggt caccgatcgc gttcaggcgc
    12361 gcccggtgcc atcggcgctg gaaaaagcgc cagccaaaaa agaagcgtat cgctggaagg
    12421 cgaccactcc agtgatgcag caaaaagaag tggtcgccac gccgaaggcg ctgaaaaaag
    12481 cgctggaaca tgaaaaaacg ccggaactgg cggcgaagct ggcggcagaa gccattgagc
    12541 gcgacccgtg ggcggctcag gtgagtcaac tttcgctacc aaaactggtc gaacaggtgg
    12601 cgttaaatgc ctggaaagag gagagcgaca actcagtatg tctgcatttg cgctcctctc
    12661 agcggcattt gaacaaccgc ggcgcacagc aaaaactggc tgaagcgttg agcacgttaa
    12721 aaggttcaac ggttgaactg actatcgttg aagatgataa tcccgcggtg cgtacgccgc
    12781 tggaatggcg tcaggcgata tacgaagaaa aacttgcgca ggcgcgcgag tccattattg
    12841 cggataataa tattcagacc ctgcgtcgat tcttcgatgc ggagctggat gaagaaagta
    12901 tccgccccat ttgatcgtaa gcacagctta cgttcgtcat ccttaacgtg attgagagag
    12961 aaacctatgt ttggtaaagg cggtctgggt aacctgatga agcaagccca gcagatgcaa
    13021 gaaaaaatgc agaaaatgca ggaagagatc gcgcagctgg aagtcaccgg cgaatctggc
    13081 gcaggtctgg taaaagtgac catcaacggt gcacacaact gccgtcgcgt agagatcgac
    13141 ccgagcctgc tggaagacga caaagagatg ctggaagacc tggtggctgc agcattcaac
    13201 gacgcagcac gtcgtattga agaaacgcag aaagaaaaaa tggcctctgt atcctccgga
    13261 atgcagctgc cgcctggctt taagatgccg ttctgatgca aaccagcccg ctgttaacac
    13321 agcttatgga agcactgcgc tgtctgccgg gcgttggccc gaagtcggcg cagcgtatgg
    13381 cgttcacgct gcttcagcgc gatcgtagcg gcgggatgcg tctggcgcag gcgctcaccc
    13441 gggcgatgtc ggaaatcggc cactgcgccg attgccgcac cttcaccgaa caggaagtct
    13501 gtaacatctg ttcgaatcca cgtcgtcagg aaaacggtca aatctgcgtg gtggagagtc
    13561 cggcggacat ttacgccatt gagcagacgg ggcagttttc aggtcgttat tttgtgttga
    13621 tgggacacct gtcaccgctg gacggcatcg gtcctgatga tatcgggctt gatcgtctgg
    13681 aacagcgtct ggcagaggaa aaaatcactg aagtgatcct cgccaccaac cccacggttg
    13741 aaggtgaagc taccgctaac tacattgccg agctttgtgc gcaatatgac gtggaagcca
    13801 gccgaatcgc tcatggcgta ccggttggcg gcgaactgga aatggtcgat ggcaccacgc
    13861 tgtcacattc ccttgccggg cgtcataaga ttcgttttta agcaaacgag agcaggatca
    13921 cctgctctcg cttgaaatta ttctcccttg tccccatctc tccctcatcc tgtttttaac
    13981 cttaaaatgg cattattgag gtagacctac atgaaaggac aagaaactcg tggttttcag
    14041 tcagaagtga aacagcttct gcacctgatg atccattctc tctattccaa taaagaaatc
    14101 ttcctgcgtg agcttatctc taacgcctcc gatgcggcgg acaagctgcg tttccgcgcg
    14161 ctttctaacc cggacctgta cgaaggtgat ggtgaactac gcgttcgtgt ctctttcgat
    14221 aaagacaagc gcacgctgac catctccgat aacggcgtgg ggatgacccg cgacgaagtg
    14281 attgaccatc tggggactat cgctaaatcc ggcaccaaat cattcctcga atccctgggt
    14341 tctgaccagg cgaaagacag ccagctgatc ggtcagtttg gtgttggttt ctactctgcg
    14401 tttatcgtgg ctgacaaagt gaccgtgcgt actcgcgcag caggcgaaaa accagaaaat
    14461 ggcgtcttct gggaatcggc tggtgaaggt gaatacactg ttgccgacat caccaaagaa
    14521 gatcgtggta ctgaaatcac cctgcatctg cgtgaaggcg aagacgagtt cctcgatgac
    14581 tggcgtgtgc gttccatcat cagcaaatat tccgaccata tcgcgctgcc ggtagagatc
    14641 gaaaaacgcg aagagaaaga cggcgaaacc gttatctcct gggagaaaat caacaaagcg
    14701 caggcgctgt ggactcgtaa caagtcggaa atcaccgatg aagagtacaa agagttctac
    14761 aaacacatcg cccacgactt taacgatccg ctgacctgga gccacaaccg cgttgaaggt
    14821 aagcaggagt acaccagcct gctgtacatt ccgtctcagg caccgtggga tatgtggaac
    14881 cgcgatcata aacacggtct gaaactgtat gttcagcgtg tgttcatcat ggacgacgca
    14941 gaacagttca tgccgaacta tctgcgcttc gtgcgtggtc tgattgactc cagcgatctg
    15001 ccgctgaacg tttcccgtga aatcctccag gacagcacgg taacgcgtaa cctgcgcaat
    15061 gcgctgacca agcgtgtgct gcaaatgctg gaaaaactgg cgaaagacga cgcggaaaaa
    15121 taccagacct tttggcaaca gtttggcctg gtactgaaag aaggtccggc ggaagatttc
    15181 gctaaccagg aagcgatcgc caaactgctg cggtttgcgt ctacccatac cgattcttct
    15241 gcgcagaccg tgtctctgga agactacgtt tcccgcatga aagaagggca ggagaaaatc
    15301 tactacatca ctgcagacag ctacgcggca gcgaagagca gcccgcacct ggaactgctg
    15361 cgtaagaaag gcatcgaagt tctgctgctt tccgaccgca tcgatgagtg gatgatgaac
    15421 tatctgactg agttcgacgg taaaccgttc cagtctgtgt ctaaagttga cgagtcgctg
    15481 gaaaaactgg ctgacgaagt tgatgagagc gcgaaagaag cggagaaagc actgactccg
    15541 tttatcgacc gtgtgaaagc cctgctcggc gagcgcgtga aagatgtccg tctgactcac
    15601 cgtctgaccg atacgccagc gatcgtttcg accgacgcgg acgaaatgag cactcagatg
    15661 gcgaaactgt tcgctgcggc gggccagaaa gtgccagaag tgaaatacat cttcgaactg
    15721 aacccggatc acgtactggt gaaacgtgcg gcagatactg aagatgaagc caagttcagc
    15781 gagtgggtag aactgctgct ggatcaggcg ctgctggcag aacgcggcac gctggaagat
    15841 ccgaacctgt ttattcgtcg tatgaaccag ctgctggttt cctgatgtca tgccggatga
    15901 cctttgtgtc atccggcatt tttcttttca tcatctgcac tttccgcaaa ttatctcgcc
    15961 attaaccgtt tcagccccag gtgcctttct tgaggcaatc gcctgttggt ggtatcgttt
    16021 atcgcttttt caaaaaattc gacacatttt aaggggattt tcgcaatgcg tatcattctg
    16081 cttggcgctc cgggcgcggg gaaagggact caggctcagt tcatcatgga gaaatatggt
    16141 attccgcaaa tctccactgg cgatatgctg cgtgctgcgg tcaaatctgg ctccgagctg
    16201 ggtaaacaag caaaagacat tatggatgct ggcaaactgg tcaccgacga actggtgatc
    16261 gcgctggtta aagagcgcat tgctcaggaa gactgccgca acggtttcct gttggacggc
    16321 ttcccgcgta ccattccgca ggcagacgcg atgaaagaag cgggcatcaa tgttgattac
    16381 gttctggaat tcgacgtacc ggacgaactg attgttgacc gtatcgtagg ccgccgcgtt
    16441 cacgcgccgt ctggtcgtgt ttatcacgtt aaattcaatc cgccgaaagt agaaggcaaa
    16501 gacgacgtta ccggtgaaga gctgactacc cgtaaagatg atcaggaaga gaccgtacgt
    16561 aaacgtctgg ttgaatacca tcagatgact gcaccgctga tcggctacta ctctaaagaa
    16621 gcggaagcgg gtaacaccaa atacgcgaaa gttgacggca ccaagccggt ggctgaagtt
    16681 cgcgcagctc tggaaaaaat tctcggctaa tcaagccgca gcagatctgc tttcgggcag
    16741 gtctgctcat cctcctcagg caatacctct cacagcaatt agttcttctt cttcactttt
    16801 ccgctacaat tatcaacaag ttgaatcgat aagaggcggt aatgcgtcag acaaaaaccg
    16861 gtatcctgct ggcaaacctg ggtacgcccg atgcccccac acctgaagcg gtaaaacgct
    16921 atctgaaaca atttttaagc gacagacgtg tggttgatac ctcacggttg ttatggtggc
    16981 cattgctgcg cggcgtgatt ttgccactgc gctcgccgcg tgtggcgaag ctgtatgcct
    17041 ctgtctggat ggaaggtggc tcgccgctga tggtttacag ccgccagcaa cagcaggcgc
    17101 tggcacaacg tttaccggag acgcccgtag cgctgggaat gagctacggc tcgccatcac
    17161 tggaaagcgc cgtagatgaa ctactggcag agcatgtaga tcatattgtg gtgctgccgc
    17221 tttatccgca attctcctgt tcaacggtcg gtgcggtatg ggatgaactg gcacgcattc
    17281 tggcgcgcaa acgtagcatt ccggggatat cgtttattcg tgattacgcc gataaccacg
    17341 attatattaa tgcactggcg aacagcgtac gtgcttcttt tgccaaacat ggcgaaccgg
    17401 atctgctgtt gctctcttat catggcattc cccagcgtta tgcagatgaa ggcgatgatt
    17461 acccgcaacg ttgtcgcaca acgactcgcg aactggcttc cgcactgggg atggcaccgg
    17521 aaaaagtgat gatgaccttt cagtcgcgct ttgggcggga accctggctg atgccttata
    17581 ccgacgaaac gctgaaaatg ctcggagaaa aaggtgtagg tcatattcag gtgatgtgcc
    17641 cgggctttgc tgcggattgt ctggagacgc tggaagagat tgccgagcaa aaccgtgagg
    17701 tcttcctcgg tgcgggcggg aaaaaatacg aatatattcc ggcacttaat gccacgccgg
    17761 aacatattga aatgatggct aatcttgttg ccgcgtatcg ctaaagctga gcggtaaaga
    17821 actgagcgcc gtcgcgaaga gcctcgtcgg cggttttcat catccgtgaa taatgcaaaa
    17881 aggcgtgcag cgtgcctggg tagagtttga actcacaggg ctgctgatgc gccgctaacg
    17941 tctggtaaag cagacggctg tcatccagca gcggatcgaa ctccgccccg gcaataaaac
    18001 agggcggaac ttcgcgagtg agatcattat taaacaggca gtaatacggc gactcgcggt
    18061 ccgcgtcgtt gcttaaatac gcctcttcat acatctgtaa atcctgttgc gttaagccat
    18121 cccagacacc gcccaacaga cgacgagtca cggaatcccg taatccgtaa agcccatacc
    18181 acagcaaaac gcccgcaact ttaccgcaat cgatctgttt atcacgcaac cacaacgcac
    18241 tggcgagcgc caacatggca cctgcggaat caccggtaaa gccaatgcgg gacatattga
    18301 tttgataatc ttccgcctgc tggtggaaat aacaacaggc agccacaatt tcctctattg
    18361 cttgcggaaa acgcgcttca ggtgaaaggg tgtaatcaat gccaatcacc gtacattggc
    18421 tgtagcttgc cagcaggcgc atgatgcgat cgtgggtatc aagattgccg agaataaaac
    18481 cgccgccatg caaataaaat agcgtcgccg ggctatctgg ctgcggacaa aagagacgtg
    18541 tttccacctg cccatatttt gttggaacca tgtaagctct ggttgccatt tctggagcgc
    18601 ccgcattcca gaatcggcgc tcaagcgtgt aatactgtcg ttgctcagca atcgttcccg
    18661 ttgcgggcca gggcggtaaa tccggctgaa gagtattcac aacggtcttc atttcagcag
    18721 aaataaggtc cagaacgggt aatttgtttt ccggcttcat gcaaaaactc ctttcaaatt
    18781 acgtcattgt aaggaaccac tgccatgaaa atgcgatccc gcctgctgat attgaaactg
    18841 gctgcgtctc gcgcgctccc gtcagattgt gttaacattc gccgctcagt taaccacccg
    18901 taaaaacaac catgaaattt cccggcaaac gtaaatccaa acattatttt cccgtaaacg
    18961 cacgcgatcc gctgcttcag caattccagc cagaaaacga aaccagcgct gcctgggtag
    19021 tgggtatcga tcaaacgctg gtcgatattg aagcgaaagt ggatgatgaa tttattgagc
    19081 gttatggatt aagcgccggg cattcactgg tgattgagga tgacgtagcc gaagcgcttt
    19141 atcaggaact aaaacagaaa aacctgatta cccatcagtt tgcgggtggc accattggta
    19201 acaccatgca caactactcg gtgctcgcgg acgaccgttc ggtgctgctg ggcgtcatgt
    19261 gcagcaatat tgaaattggc agctatgcct atcgttacct gtgtaacact tccagccgta
    19321 ccgatcttaa ctatctacaa ggcgtggatg gcccgattgg tcgttgcttt acgctgattg
    19381 gcgagtccgg ggaacgtacc tttgctatca gccccggcca catgaaccag ctgcgggctg
    19441 aaagtattcc ggaagatgtg attgccggag cctcggctct ggttctcacc tcttatctgg
    19501 tgcgttgcaa gccgggtgaa cccatgccgg aagcaaccat gaaagctatt gagtacgcga
    19561 agaaatataa cgtaccggtg gtgctgacgc tgggcaccaa gtttgtcatt gccgagaatc
    19621 cgcagtggtg gcagcaattc ctcaaagacc acgtctctat ccttgcgatg aacgaagatg
    19681 aagccgaagc gttgaccgga gaaagcgatc cgttgttggc atctgacaag gcgctggact
    19741 gggttgatct ggtgctgtgt accgcagggc caatcggctt gtatatggcg ggctttaccg
    19801 aagacgaagc gaaacgtaaa acccagcatc cgctgctgcc gggtgctata gcggaattta
    19861 accagtatga gtttagccgc gccatgcgcc acaaggattg ccagaatccg ctgcgtgtct
    19921 attcgcacat tgcgccgtac atgggcgggc cggaaaaaat catgaacact aatggagcag
    19981 gggatggcgc actggcagcg ttgctgcatg acattaccgc caacagctac caccgtagca
    20041 acgtaccaaa ctccagcaaa cataaattca cctggttaac ttattcatcg ttagcgcagg
    20101 tgtgtaaata tgctaaccgt gtgagctatc aggtactgaa ccagcattca cctcgtttaa
    20161 cgcgcggctt gccggagcgt gaagacagcc tggaagagtc ttactgggat cgttaagtta
    20221 tcgaccagac cggacgacga tggcatccgt aggccggata agatgcgtca gcatcgcatc
    20281 cggcaatgga atcaatgttt taccccgtca ccacctcacc cgccggtggc gtttccagca
    20341 gttccagcat agtacgagca atttcacgct cacccatcac tacctgattc gcaccacgtt
    20401 cggtgatata cgccacttca tcgtcataat gggcgcgggc aataatctca atatccggat
    20461 ttttcgcgcg ggcagatgcc acaatctcgc ccgcttcata accgttggga atcgtcagga
    20521 tcagccattt tgcacattcc agatgcgcca gttgcataat ttcttcgttc gccgcattgc
    20581 ccaatactgc gcggaccccg cgctctcgca gttcatcaac acgggttcgt gacgtctcaa
    20641 tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg cctacacgac
    20701 cgtaacctac cagtagcgca tggttgcaaa tatccactgg gatctgcttc tcttcttcga
    20761 tagcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat ttctccagca
    20821 gcgcaaacag gaccgggttg agcataatcg acaaaatcgc ccctgccagt accaggttct
    20881 gcccggcttg cggcaataga tccagcgcca tgcccagtcc cgccaggata aacgcgaact
    20941 caccaatttg cgccaggctg gcggcgatag ttaatgccgt acgttgggag tgaccaaaca
    21001 gtcgcaccag gaaaaacgcg gctaacgact taccaaacag aataatcgcc agcgtcgcca
    21061 gcactgccag cggttgctga atcagaatta acggatcaaa caacatcccg acggagacaa
    21121 aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc gtgggcggca cggtgactca
    21181 gttcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag gagacatcaa
    21241 acagctccac cgcaccaaag gcaatcccta acgccagcgc cagcaccgac agggtaaaca
    21301 gctcacggga accggttgct gcgctacgtg ccataatcca cggcaccaga cggcgaccta
    21361 ccagcatcat aatggcgata aatgcgatca ccttgccgat ggtgatcccc atatcgacag
    21421 caagagtggc aaagcccaca tcgccttgtt ccatcattcc tgccactgcg ggcagcaaca
    21481 ccagcgtcag aaccatgacc aggtcttcca caatcaacca accgatggcg atttgcccac
    21541 gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag taacaccacg gtactggcgg
    21601 tggaaaggca taagccgaac acgataccgg tcattaacga ccagcccagc acggcggaga
    21661 gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcaccggga atggcgatgg
    21721 cctttaccgc catcaaatcc ttcagcgaaa agtgcagacc cacgccaaac atcaacagaa
    21781 tgacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag cccggagtga
    21841 atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata cgtagtttat
    21901 tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg gtgataagcg
    21961 gggtggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt ttggccggga
    22021 aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttta tgaataattg
    22081 ttgaattttg cagaaaaatg gaattagctg caaaaaaagc acggatcgga aaacagaagg
    22141 cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga tgcagccaaa gtttgccttt
    22201 ggctgcaatg aaatcagtct ttatgccggt tatcaggcag gaatatggtc aacatcccca
    22261 atagtggcag gaaagcacag attttataga ctaactcgat gctggtgtga tcggcgataa
    22321 gccccagaac tgccgctccc agacctccca tgccaaaagc aaaaccgaaa aagagtccag
    22381 aaaccatacc gatacgtccc ggaagtagct cctgagcgta gaccagaatg gcagagaatg
    22441 ccgaagcgag gataaatcca ataatcaccg ttaaaacccc cgtccagtgc aggctggcgt
    22501 agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga gccccaaatc acatatttcc
    22561 gtccaatttt atcccctaca ggcccgccaa tcaccgtacc tgccgcaacg gcaaacagga
    22621 aggcaaacaa atgaagctga gcattctgga tagataatcc gaatttttgc atcagataaa
    22681 aggtgtaata gctgctgatg ctcgccatat agaaatattt cgagaaaatg aggattaaca
    22741 gaatgctgac cgccagaaca actttattgc gcggcagtgg attgataatc gtcgctttgg
    22801 gttttccttt attcattcgg tgctgtgccg agtaccaacg gctgatttgc gccaacacca
    22861 cgatcgccag cagtgccgca agcacaaacc aggcaacgtt gcctttgcca taaggcgcga
    22921 taatcaccgc cgccagcaag gggcccaggg aactgccaaa gttgccgccg acctgaaaga
    22981 tagattgcgc caggccatga cgcccgccgg aagccatacg ggccacgcga gaagattccg
    23041 gatgaaagac cgatgaaccg gtaccgacca gcgccgctgc cagcagaact gcgccgaaac
    23101 tgcccgccag cgccagcagc accagaccac ttaaggtaaa gcacatgcca attggcaacg
    23161 accacggcat cggatattta tcggtccagt agccgacaac tggttgcagt agcgaagagg
    23221 cgagctggaa ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa aactctgact
    23281 gaagcagcgg ataaatcgcc agaatcagcg attggatcat gtcgttcagc agatgtgaga
    23341 ggctgatagc acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac gcagccgcgc
    23401 ccgccacagg ctgggtttgt tcactcattg ccataggaaa gtcacttttt cagggttgcg
    23461 atgtaaagaa tgatcttatt tgtgattatt acccgactaa catacctgta tgcgccgcct
    23521 gaaggaagtc tcaacgctga atacagaatt tctaatctgg atacagattt atcttcaccg
    23581 gacgcagact tgtctatgat gtcgcgtcat actatttttc aacacgttga aatcaggtca
    23641 gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt agcactgtta accacattta
    23701 cactggcgag tgaagctgct ctggcgtatg agcaggataa aacctacaaa attacagttc
    23761 tgcataccaa tgatcatcat gggcattttt ggcgcaatga atatggcgaa tatggtctgg
    23821 cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa ggcggtagcg
    23881 tgctgctact ttccggtggc gacattaaca ctggcgtgcc cgagtccgac ttacaggatg
    23941 ccgaacctga ttttcgcggt atgaatctgg tgggctatga cgcgatggcg atcggtaatc
    24001 atgaatttga taacccgctc accgtattac gccagcagga aaagtgggct aagttcccgt
    24061 tgctttccgc caatatctac cagaaaagta ccggcgagcg cctgtttaaa ccatgggcgc
    24121 tgtttaagcg tcaggatctg aaaattgccg ttattggcct gacgacggat gacacagcca
    24181 aaattggtaa tccggaatat ttcactgata tcgaatttcg taagcccgct gatgaagcga
    24241 agctggtgat tcaggagctg caacagacag aaaagccaga cattattatc gcggcgaccc
    24301 atatggggca ttacgacaat ggtgagcacg gctctaacgc accgggcgat gtggagatgg
    24361 cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg tggccactcg caagctccgg
    24421 tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg ccatgcaaac
    24481 cggatcaaca aaacggcatc tggattgtgc aggcgcatga gtggggcaaa tacgtgggac
    24541 gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt taactaccag ctgattccgg
    24601 tgaacctgaa gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg ctgtacaccc
    24661 ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc accgttccag aacaaaggca
    24721 aagcgcagct ggaagtgaaa ataggcgaaa ccaatggtcg tctggaaggc gatcgtgaca
    24781 aagtacgttt tgtacagacc aatatggggc ggttgattat ggcagcacaa atggatcgca
    24841 ctggtgccga ctttgcggtg atgagcggag gcggaattcg tgattctatc gaagcaggtg
    24901 atatcagcta taaaaacgta ctgaaagtgc agccattcgg caatgtggtg gtgtatgccg
    24961 acatgaccgg taaagaggtg atcgattatt tgaccgccgt cgcgcagatg aagccagatt
    25021 caggtgccta cccgcaattt gccaacgtta gctttgtggc gaaagacggt aaactgaaca
    25081 acctgaaaat caaaggcgaa ccggtcgatc cggcgaaaac ttaccgcatg gcgacattaa
    25141 acttcaatgc caccggcggt gatggctatc cgcgccttga taacaaagcg ggctatgtga
    25201 ataccggctt tattgatgcc gaagtgctga aagcgtatat ccagaaaagc tcgccgctgg
    25261 atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa agtgccggat
    25321 gttcgcatcc ggcacaatgc ttaatcgcga cgggcgatat cagcaaattt ggcatcgagg
    25381 atctttgcca gatcgcctgc cgccagttcg atatccagcc cgcgcttgcc gccggaaaca
    25441 taaatagtgg caaattcttg tgcgggggcg tcgataatcg ttggcagacg ttttttctgc
    25501 cccagtgggc taatcccccc taccagatac cccgtcgaac gctgcgcgac catcggatcg
    25561 gccatctcaa ctttcttggc acccagcgct tttgctactt ttttaagatc cagttgacct
    25621 gcgaccggcg taacggccac ggcaaggtgt ttcatatcac cgttcactgc gaccagcagc
    25681 gttttgtaga cctgatccgg attcaaacct aattttttga cgacttcatc gccaaaattg
    25741 gtttcagccg gatcgtgctc gtaggtgtgg atttgaaacg aaatcttgtt tttttcgagt
    25801 aatttaactg cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg aagcaagcat
    25861 acgcctgatg gcggtccaaa aaaaagagtc atcttgccta agagtattgg caggatggtg
    25921 agattgagcg acaatcgagt tacaccgtcg cttaaagtga cggcataata ataaaaaaat
    25981 gaaattcctc tttgacgggc caatagcgat attggccatt tttttagcgc aacatctgcg
    26041 gcaaattccc ttctccatac aggtgtagtg caccaaccgc gaccacatag cgccccggcg
    26101 gcatggcgca taatttgtcc cgccaggcga gatttcgctg atgcatcagc acatcgtaca
    26161 gcgactgact gaacgtatta ggcagcgtta tttcattatt ttgcggcggc gcattcagcc
    26221 accagctcat catttgttgc agcaaccgtg cgttggtgtg ccagtgggtc agcgtatcgt
    26281 ccagcagcgc cagtccgttg tcagggagct ggagcaacat ggcaatctgg ttttcagccc
    26341 cttccagttc aatcacgggt ttatgttgtt gcttagccgc ctgcaatagc tggtaatcga
    26401 taccgtattc tgcccgcagc cccagttttt gcgcctgcgt cgcctgaaga accatcgcga
    26461 tttgccacag cggttgagta gaaaagagcg acggagaaat gcccatctcc tggctaatat
    26521 gctgcaggtt ttggagttgc tcctcgctaa tgcgcttttc cagggcctcg caggcaggca
    26581 aattggcaaa aggcgtatcg ctggtggaaa catccgcctc gacgatcagc gcatcggcgt
    26641 ttttgagctt tttgagcaaa cgggtgggca ggggagccat atcgtggcta cccatatgaa
    26701 tactgccgat cagatgaaaa tggcgattgc cgggaagaga gatatcgata gcaggccagg
    26761 tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac ccggtacaac agatccatac
    26821 gacctccctt tgtgaaatat catgctagcg cgcggggagg gatggcgcaa atggagttga
    26881 ttgttgcact tgcgcacagt gcttgtgtgc attacaatga ttaaataatg gacaaggaga
    26941 tgagagagga atggctcaaa agaaaaatat aaggagtttt cgtgatgcct ggttggctga
    27001 tttttttgtc cattcaacgc cacatcgaaa gatccctgct gaaatccata ccacgttatc
    27061 acgaaagttg gacatcatta atgctgccac ttctcatcgg gatttacgct cccctccggg
    27121 gaaccgatac gaagagttat ccgggaaatt acaggaatac tcctccatta gagtgaataa
    27181 acagtaccga ctgattttta aatgggttaa tggcaaggcc gaagaattgt ttttagaccc
    27241 acacaactac taaaacatag ctgtcgtatt gacgagaaag gtaaaactat gaaacaggca
    27301 acaagaaaac cgacgacacc cggagatatt cttctctatg aatatctgga accgctcgat
    27361 ttgaaaatca atgagttagc agagttgctg catgttcatc gtaatagcgt cagtgcactg
    27421 atcaataaca atcgtaaact caccactgag atggcattcc gtctggcgaa agtttttgat
    27481 accacagtcg atttttggct aaacctccag gcggcagttg atctttggga agttgaaaac
    27541 aacatgcgta cccaggaaga attgggacgg attgaaacag tggctgaata tttggcacgc
    27601 cgtgaagagc gtgcaaaaaa ggtcgcgtag aataatttcc ctataacgga tgcgccaccc
    27661 taaagcagtg catccgcaat gatgtactta ttccttcggt ttaaaccgca gcaaccggtt
    27721 ggcgttactc actacggtaa tcgacgagag cgccattgcc gctccggcga ccaccgggtt
    27781 aagcagcgta ccggtgaacg gccacaaaat accggcggcg accggaatac caatgctgtt
    27841 gtagataaac gcaccgagca ggttctgctt catattgcgc agcgttgcgc gggaaatcgc
    27901 gagcgcatcc gcaacaccca tcaggctatg gcgcatcagg gtaatcgccg cggtttcaat
    27961 ggcaacatca ctgccgccac ccattgcaat gccgacatcc gcctgagcca gcgctggcgc
    28021 gtcgttaatg ccatcgccca ccattgccac ctgacgtcct tcactttgca gacgtttgat
    28081 cgcttcggct ttaccgtccg gcagcacccc ggctatcacc tcatcaatcc ctgcttcttt
    28141 ggcgatcgca ttggcggtgg ttgggttatc cccggttagc atcaccagac gatatcccgc
    28201 tttatgcagg cgttgcagcg ccgccacgct atcactacgc aatggatcgc gtacagccag
    28261 cagggctacc gctttcccgt caaccgccag cagcacaggc gttgcccctt gcgatgcctg
    28321 agctgaaata tctgcttcaa tcgctttagt atcaacttgc tggtcattta acagcgcctg
    28381 attgcccagc aataacgcat gaccttcagc ttcaccgctc acgcccagcc cgcgcaatgt
    28441 gcggaaaccg ttgacctgcg gcagctgcat atcgcttgct ttatcgagga tcgcccgtgc
    28501 cagcggatgg ctggaacctt gttccagtgc cgccgccaga cgcagcgcct gcgcttcgtc
    28561 aaaatcagca aatgttttta ctgcgacaac ctgcggcttc ccttcggtca gcgtcccggt
    28621 tttatcgaac accacggtgt cgagcgtact ggcgcgttgc agcgcgtcgg cgtcccgcac
    28681 cagcacgcca aactcagccg cccgcccgac gcccgaaata atcgacatcg gcgtcgccag
    28741 tcccagcgca cacggacagg cgataatcag caccgtggtg gcaatcacca gggtataaac
    28801 aatttgcggt gccgggccaa agaaatacca gattgccgca ctgaccagcg caatgactac
    28861 caccaccggc acaaacacag cggagatttt atccgccagt tgaccgattt ctggcttgct
    28921 gctctgggcc tgtcgcacca tgcgaatgat ccgtgacagc gtagtatggc tgccgaccgc
    28981 actggcgcga aacagcacac tgccgtcctg taccactgtc ccggcatgga cgctatcgcc
    29041 ttcgcctttt tgctgcggga ttggttcgcc cgtcagcatc gcttcatcca gccacgcttc
    29101 gccctgggta atttcaccat ctaccggcac ccgatcaccc gtcgtcaggc gcagcaacat
    29161 acctggctga acatctgcca gcggcacgtt tttttcacct tcgttggtga ccaggcgcgc
    29221 cgtcggtggg gttaaatcga gtaatttctc cagcgcctta gaagaacgct gacgtgcgcg
    29281 cgcttccagc atatgaccga gattaatcag accgataatc atcgcactgg cttcatagta
    29341 aagatgtcgc gcttccatcg ggaaccactg cggccacagg ttgacgctca ttgaatagag
    29401 ccacgccacg cccgtaccca gcgccaccag cgtatccatc gtcgctgcac cgttcagcag
    29461 gcttttccat gcgctgcggt aaaaatggcc gccggcgaaa accattaccg ccagggttat
    29521 caggccgata accaaccaca ggctgcggtt gtcggcggtg accatcatgt tatcgccgat
    29581 catcccccag accatcaccg ggatacccac cgccagtgcg acaatcgcct gccagcggaa
    29641 gcgcttcatc gtagcgacgg cggtttcttg ctggcgctcg cggcgtttag cgtcatcttc
    29701 aatcgcttcc gcgccgtagc ccgctttttc caccgcctgc actaaatctt gtggggaggc
    29761 actgcccatc accagcgcag tacgctccgc caggtttacc cgtgcctgag tgacgcccgg
    29821 tacgctttgc aacgcatttt gtacgcgggt gacacagctg gcgcagctca tgccgctcag
    29881 cagcaactgc tggctgtcat cgtcatcggc ggtcgctgcc ggaagcgcct cagaaaccgc
    29941 tgtcagtgct tccgacggga ttgatgactc cgccagcggt ttagcctttg ggtggcttac
    30001 agatgcgtca taacccgctt gtttgatggt ttcgatcagc tgttctgcac tggcagtccc
    30061 ggtaacgtgc gcttcagtga cagacacatc cgcctgctca acatccgggc gctgttcgag
    30121 actttctttc acgcgtttaa cgcagtgacc gcaggacagg ccgtccaggg tcaggtcgat
    30181 agtttgtgac ataaaacact ccttttaaga cagttttgac tggctgtgat aaaggttaaa
    30241 ccttccagca aggggaaggt caagaaatta ataaaccagg cgggtaaaag tacgtaaaga
    30301 ttaaaaaatc ggctcgattt gcatcaggat tagacattta tctctttgtt ttcctgtagt
    30361 taagttgcgg gtgctaagtt aaatcaggat gcctgaaaat tggcaccggg gtgaggaatt
    30421 acctcccgca tctataaaaa ggagttaaca aaagatgtta gatgcaaaca aattacagca
    30481 ggcagtggat caggcttaca cccaatttca ctcacttaac ggcggacaaa atgccgacta
    30541 cattcccttt ctggcgaatg tacctggtca actggcagca gtggctattg tgacctgcga
    30601 tggcaacatc tatcgcgcgg gtgatagtga ttaccgcttt gcgctggaat ccatctcgaa
    30661 agtctgtacg ttagcccttg cgttagaaga tgtcggcccg caggcggtac aggacaaaat
    30721 tggcgctgac ccgaccggat tgccctttaa ctcagttatc gccttagagt tgcatggcgg
    30781 caaaccgctg tcgccactgg taaatgctgg cgctattgcc accaccagcc tgattaacgc
    30841 tgaaaatact gaacaacgct ggcagcgaat tttacatatc caacaacaac tggctggtga
    30901 gcaggtagcg ctctctgacg aagtcaacca gtcggaacaa acaaccaact tccataaccg
    30961 ggccattgcc tggctgctgt actccgccgg atatctctat tgtgatgcaa tggaagcctg
    31021 tgacgtgtac acccgtcagt gctccacgct catcaatact gttgaactgg caacgcttgg
    31081 cgcgacgctg gcggcgggtg gtgtgaatcc gctgacgcat aaacgcgttc ttcaggccga
    31141 caacgtgccg tacattctgg ctgaaatgat gatggaaggg ctgtatggtc gctccggtga
    31201 ctgggcgtat cgcgttggtt taccgggcaa aagcggtgta ggtggcggta ttctggcggt
    31261 cgtccctggc gtgatgggaa ttgccgcgtt ctcaccaccg ctggacgaag aaggcaacag
    31321 tgttcgcggt caaaaaatgg tggcatcggt cgctaagcaa ctcggctata acgtgtttaa
    31381 gggctgatca tgatgaacac ggaaggtaat aacgataaca aacccctcgg tctatggaac
    31441 gtcgtttcca tcggtattgg ggcaatggtg ggggcgggga tcttcgcgct gctggggcag
    31501 gctgcgttgc taatggaagc ctcgacctgg gtcgcctttg cttttggcgg tattgtggcg
    31561 atgttttccg gttatgccta tgcgcgcctg ggggcgagct atcccagcaa tggcggtatt
    31621 atcgacttct ttcgtcgcgg attaggcaac ggcgtctttt cgctggcact ctcgttattg
    31681 tacctgttga cgctggcggt gagcatcgcc atggtcgccc gtgcttttgg cgcttatgcc
    31741 gtgcagtttt tgcatgaagg cagtcaggag gagcacctta tattgctcta cgcgttgggg
    31801 atcattgcgg tgatgacgct tttcaactcc ttaagcaacc atgcggtagg gcggctggaa
    31861 gtgatcctcg tcggcattaa aatgatgatc ctgttattgc tgatcattgc cggtgtctgg
    31921 tcgctgcaac cggcgcatat ttccgtctct gcgcccccca gctccggtgc gttcttctcc
    31981 tgtatcggga taaccttcct tgcctatgcg ggctttggca tgatggcgaa cgcggcggat
    32041 aaagtgaaag atccgcaggt cattatgcca cgggcgtttc tggtggcgat tggcgttacc
    32101 acgttgcttt atatctcgct ggcgctggtt ttgcttagcg atgtatcggc attagagtta
    32161 gaaaaatatg ccgataccgc cgtagcgcag gctgcttctc cgctgctcgg gcatgtgggt
    32221 tatgtgatcg tcgtcatcgg cgctttactg gcgacggctt cagccataaa cgcgaacctg
    32281 ttcgccgtgt ttaacatcat ggacaacatg ggcagcgaac gcgaactgcc gaagctaatg
    32341 aataaatccc tgtggcggca gagtacctgg ggcaacatca ttgtcgtggt gctgattatg
    32401 ctgatgacgg cggcgctgaa tttaggctca ctcgccagcg ttgccagcgc cacctttttg
    32461 atctgctatc tggcggtgtt tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg
    32521 ttgccgattc ttatcgttgg tacgttggtt atgttgttgg tgatcgttgg ctttatctac
    32581 agtctctggt cccagggcag ccgtgcgctg atatggatta ttggcgcact cttactcagc
    32641 cttattgtgg caatggtcat gaagcgcaat aaaactgtat aacatctctc tgtgcgcagt
    32701 acctcctgta ttattgtggt ggcggtcgat attcgcactg gcaaaaaaac gtgcttgaat
    32761 atctgttgaa accctttaac aaagcacagg aggcgttgcg cgaacgatga acattagcga
    32821 tgtagcaaaa attaccggcc tgaccagcaa agccattcgc ttctatgaag agaaggggct
    32881 ggtgacgccg ccgatgcgca gcgaaaacgg ttatcgcacc tacacgcagc agcatctcaa
    32941 cgaactgacc ttactgcgcc aggcacgcca ggtgggcttt aacctggagg agagcggcga
    33001 actggtgaat ctgtttaacg atccgcagcg tcacagcgcc gacgtcaaac gtcgtacgct
    33061 ggagaaagtg gcggagatcg aacagcatat cgaagaactg caatccatgc gtaaccagct
    33121 gctggcgctg gcgaatgcct gccccggtga tgacagcgcc gactgcccga ttatcgaaaa
    33181 tctctccggc tgctgtcatc atcgggcggg gtgattaagg cttgcgaaaa tagtcgtcca
    33241 ttattttcgt cagctccgga cgttccccgt catcattgat ggcaaaatcc agaaaaaaag
    33301 catctgcttg cggcgttcgg ttttgcgcca tcaaccggaa ggtgaggggg cctgaccagg
    33361 tttcggatgg gctgatgtgc ttatcagggc cgaaactctc ccatgcgttg ggcggtaatg
    33421 ttttgcacgc ggtgagtttt ggtatgtggt attgcagctc tgttttgtgc agacattccc
    33481 cgccaggaaa taccagggtt tgttgctcat cattaaaatg ggttgccgtg ctgagcacca
    33541 gtgttttgag cggtcgccct tccagcgcgg cggcagtagc cttatggtgt ccgtcgagca
    33601 gaacgcagag ataggaatca gggagatacc aggccagagc gcgggcgatt ccagttttcg
    33661 agagcagaca aaaaccgggc ggctccctgt tcaccctccg caaaaccgct gtagacaaat
    33721 tccgcacagg tagggcaacc agcctcggac cagaaacgta ttaacgggac atccttgcca
    33781 gcagagcgaa tggataagac cgtgcgagtg cggatcgtaa atcacacatt gacgcagaaa
    33841 tcctttcccg gacttcaccg aaaaaatgac gcccgcagcg gtatttttca gcagacgtac
    33901 ttcaggtttt cgaaagggga aaaagcatgg ccactcctta gccaaaattt cagttccctg
    33961 aactgtgcca gaaaattacg ctggtgacta ttgctctgga aatggaaaca gtgtccttac
    34021 ccattaatcc ccgtttgtac cgaaagaata tgttgcaaac tttctaccat aatgcgcccc
    34081 gactgaaata agccttcctg gatttcattg ttaacattca cagcgtgaac cagggaggct
    34141 aataacacca cttgcaagaa ggcaaaaata aacgcaccga taatcataat atgcgtcagg
    34201 gggattttcc tttgctcctt cattttaaat aacccacaaa ctggccttct ttcattaagg
    34261 cgacttttcc tgtgtgatga taaacaatga cattatacag acccgactga taaaccgtgg
    34321 catcggcatt atcttcatct atggcacata atgaatcatt gagataagca aaattcttac
    34381 tactcattct aaaggtcatg ccaaatcctt tataaatggc tttttttgcc gcctgggcat
    34441 gttgtgcggc ttctgcattt ttaaatgcct gcgcttcaga agcacttaaa atagccgtct
    34501 ggcgctgctg ttctgcgaga taacaggtgt catggctgac gccctgcgcc tgacaatctg
    34561 cgatgcggga agcgggtgag ctacagccag ccagtaatat aaccgccagc agggaaaagg
    34621 atttaatgga gctattcatc attgttaacc ttaaataatg tgcgaattaa tgaagaggtg
    34681 aaacccggca agcttccttg ctcttattta tattgaggat tgaaatatta gaacgaatat
    34741 cctacgccga cacctgcgcc gaaatcagat tgcgtatccg cagaaatacc ggctttaagt
    34801 accgtatgcg ttccggcatt aaaagatgtt ccgacagaca ccgcagattc accgttgaag
    34861 gttcccgctc ccgctgacac catcatcacg tcaccggttt taacctgtgg ttgtgaggcg
    34921 atagcgatgg cagatgcagt tcccgcattg gcttctttac ggttgtcctc aacttcgttt
    34981 ttcagcgaac tgtaatggtt attgagcgtg ttaatttgtt ggctgttatc tgcgacccgt
    35041 ttttgcgtgt tgcttaactg ttgctgcgtt tgtgcggaat cttgtctcag actttcaatt
    35101 tgctcatgtg cctggacaga cttctgcgcc agactttgca tttgtttatc gtattgagcc
    35161 ctttcttttt gctgttgtgc tgccacatcg gttgtcagtg cattcatttt ctgcccttgt
    35221 tccgtggtag tgcgctgatt agcctcattt tgagccgcca gtctggcatt aatcatctcc
    35281 tgataatgcg cagtattttg cgtatctcca gtcgcggcga gcgttttttg tgtggctacg
    35341 atctgcgcct gttgctctgt aatttgcgtt tgctgggcat cagttaccgc agactgtcgg
    35401 gcgttgatca tgctctgata acgggcaggt gtctgtgtat cacctgtagc ggcaacggtt
    35461 ttttgtcctg cgtcaggatc tgtctggaca gtgggagtct gagtggtatc aggtgtttca
    35521 gaagggatgt agtgatgttc agtcaaatag gtatacgcag cggggctgtt gtctgtgcgt
    35581 tctaaaaagc gggtaatctt ctgctcaatc tccgattgcg acatgccaga gttaatggct
    35641 gaaagctctg ttgccgccgc ttcaatggta tcaccagcta aaacaacagg gctggatgtt
    35701 gctgaaatta tgagtgccag taaagctacg tttacagttt tcattcat
//
LOCUS       DAZKJR010000036        35053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKJR010000036 DAZKJR010000000
VERSION     DAZKJR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.036"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..96
                     /gene="ymgJ"
                     /locus_tag="ACHOWS_001804"
     CDS             <1..96
                     /gene="ymgJ"
                     /locus_tag="ACHOWS_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFQ0047382.1"
                     /translation="YQKRVIFYPTTLQRTAFKKCLFLMRDIIISS"
     gene            57..176
                     /locus_tag="ACHOWS_001805"
     CDS             57..176
                     /locus_tag="ACHOWS_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0047383.1"
                     /translation="MPLSYAGYHYFIMMSLVSGEHNTLALPIQEQSPGLVPAG"
     gene            211..555
                     /locus_tag="ACHOWS_001806"
                     /pseudo
     CDS             211..555
                     /locus_tag="ACHOWS_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(920..1186)
                     /gene="minE"
                     /locus_tag="ACHOWS_001807"
     CDS             complement(920..1186)
                     /gene="minE"
                     /locus_tag="ACHOWS_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFQ0047384.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1190..2002)
                     /gene="minD"
                     /locus_tag="ACHOWS_001808"
     CDS             complement(1190..2002)
                     /gene="minD"
                     /locus_tag="ACHOWS_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFQ0047385.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2026..2721)
                     /gene="minC"
                     /locus_tag="ACHOWS_001809"
     CDS             complement(2026..2721)
                     /gene="minC"
                     /locus_tag="ACHOWS_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFQ0047386.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3240..3608
                     /gene="ycgJ"
                     /locus_tag="ACHOWS_001810"
     CDS             3240..3608
                     /gene="ycgJ"
                     /locus_tag="ACHOWS_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFQ0047387.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3711..4112)
                     /gene="pliG"
                     /locus_tag="ACHOWS_001811"
     CDS             complement(3711..4112)
                     /gene="pliG"
                     /locus_tag="ACHOWS_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFQ0047388.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4354..4647
                     /locus_tag="ACHOWS_001812"
     CDS             4354..4647
                     /locus_tag="ACHOWS_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFQ0047389.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4719..5378
                     /gene="ycgM"
                     /locus_tag="ACHOWS_001813"
     CDS             4719..5378
                     /gene="ycgM"
                     /locus_tag="ACHOWS_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFQ0047390.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5470..5916
                     /gene="ycgN"
                     /locus_tag="ACHOWS_001814"
     CDS             5470..5916
                     /gene="ycgN"
                     /locus_tag="ACHOWS_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFQ0047391.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6123..6449)
                     /gene="hlyE"
                     /locus_tag="ACHOWS_001815"
                     /pseudo
     CDS             complement(6123..6449)
                     /gene="hlyE"
                     /locus_tag="ACHOWS_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            6710..7129
                     /gene="umuD"
                     /locus_tag="ACHOWS_001816"
     CDS             6710..7129
                     /gene="umuD"
                     /locus_tag="ACHOWS_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFQ0047392.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7129..8397
                     /gene="umuC"
                     /locus_tag="ACHOWS_001817"
     CDS             7129..8397
                     /gene="umuC"
                     /locus_tag="ACHOWS_001817"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFQ0047393.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(8442..8972)
                     /gene="dsbB"
                     /locus_tag="ACHOWS_001818"
     CDS             complement(8442..8972)
                     /gene="dsbB"
                     /locus_tag="ACHOWS_001818"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFQ0047394.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9118..10659)
                     /gene="nhaB"
                     /locus_tag="ACHOWS_001819"
     CDS             complement(9118..10659)
                     /gene="nhaB"
                     /locus_tag="ACHOWS_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFQ0047395.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            10881..11600
                     /gene="fadR"
                     /locus_tag="ACHOWS_001820"
     CDS             10881..11600
                     /gene="fadR"
                     /locus_tag="ACHOWS_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFQ0047396.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11652..13184)
                     /gene="ycgB"
                     /locus_tag="ACHOWS_001821"
     CDS             complement(11652..13184)
                     /gene="ycgB"
                     /locus_tag="ACHOWS_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFQ0047397.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMSYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13514..14812
                     /gene="dadA"
                     /locus_tag="ACHOWS_001822"
     CDS             13514..14812
                     /gene="dadA"
                     /locus_tag="ACHOWS_001822"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFQ0047398.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            14822..15892
                     /gene="dadX"
                     /locus_tag="ACHOWS_001823"
     CDS             14822..15892
                     /gene="dadX"
                     /locus_tag="ACHOWS_001823"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFQ0047399.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15949..17685)
                     /gene="cvrA"
                     /locus_tag="ACHOWS_001824"
     CDS             complement(15949..17685)
                     /gene="cvrA"
                     /locus_tag="ACHOWS_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFQ0047400.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17780..18694)
                     /gene="ldcA"
                     /locus_tag="ACHOWS_001825"
     CDS             complement(17780..18694)
                     /gene="ldcA"
                     /locus_tag="ACHOWS_001825"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFQ0047401.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            18794..19405
                     /gene="emtA"
                     /locus_tag="ACHOWS_001826"
     CDS             18794..19405
                     /gene="emtA"
                     /locus_tag="ACHOWS_001826"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFQ0047402.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(19407..20141)
                     /gene="ycgR"
                     /locus_tag="ACHOWS_001827"
     CDS             complement(19407..20141)
                     /gene="ycgR"
                     /locus_tag="ACHOWS_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFQ0047403.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            20342..20596
                     /gene="ymgE"
                     /locus_tag="ACHOWS_001828"
     CDS             20342..20596
                     /gene="ymgE"
                     /locus_tag="ACHOWS_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0047404.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(20646..22616)
                     /locus_tag="ACHOWS_001829"
     CDS             complement(20646..22616)
                     /locus_tag="ACHOWS_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFQ0047405.1"
                     /translation="MRLKKRYLCTALSLAFTQQALAAQESDTLTVWSSPVSSTTTTVL
                     DHPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(22642..23496)
                     /gene="modD"
                     /locus_tag="ACHOWS_001830"
     CDS             complement(22642..23496)
                     /gene="modD"
                     /locus_tag="ACHOWS_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFQ0047406.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(23493..24305)
                     /locus_tag="ACHOWS_001831"
     CDS             complement(23493..24305)
                     /locus_tag="ACHOWS_001831"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0047407.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(24315..25073)
                     /locus_tag="ACHOWS_001832"
     CDS             complement(24315..25073)
                     /locus_tag="ACHOWS_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0047408.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGQ
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(25070..26050)
                     /locus_tag="ACHOWS_001833"
     CDS             complement(25070..26050)
                     /locus_tag="ACHOWS_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0047409.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVTV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(26050..27072)
                     /locus_tag="ACHOWS_001834"
     CDS             complement(26050..27072)
                     /locus_tag="ACHOWS_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFQ0047410.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            complement(27409..29106)
                     /gene="treA"
                     /locus_tag="ACHOWS_001835"
     CDS             complement(27409..29106)
                     /gene="treA"
                     /locus_tag="ACHOWS_001835"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFQ0047411.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
     gene            complement(29427..30845)
                     /gene="dhaM"
                     /locus_tag="ACHOWS_001836"
     CDS             complement(29427..30845)
                     /gene="dhaM"
                     /locus_tag="ACHOWS_001836"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0047412.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPSYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            complement(30856..31488)
                     /gene="dhaL"
                     /locus_tag="ACHOWS_001837"
     CDS             complement(30856..31488)
                     /gene="dhaL"
                     /locus_tag="ACHOWS_001837"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0047413.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSIPAALEAASS
                     VAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(31499..32569)
                     /gene="dhaK"
                     /locus_tag="ACHOWS_001838"
     CDS             complement(31499..32569)
                     /gene="dhaK"
                     /locus_tag="ACHOWS_001838"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0047414.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRSDAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            32797..34716
                     /gene="dhaR"
                     /locus_tag="ACHOWS_001839"
     CDS             32797..34716
                     /gene="dhaR"
                     /locus_tag="ACHOWS_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFQ0047415.1"
                     /translation="MSSAFNNDGRDISPLIATSWERCIKLMKRDTWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRACALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            complement(34753..>35053)
                     /locus_tag="ACHOWS_001840"
     CDS             complement(34753..>35053)
                     /locus_tag="ACHOWS_001840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047416.1"
                     /translation="ACYAASFNSELSKIIIRLLAILTMCLLCDNNHSALTVQEFVMKK
                     TFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMPPSNGGPNHGSHGRY"
BASE COUNT         8704 a         8618 c         9053 g         8678 t
ORIGIN      
        1 tatcagaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
       61 ctctttctta tgcgggatat cattatttca tcatgatgtc tttggtgagc ggtgaacaca
      121 atacacttgc gctgcctatt caggagcaat cccctggtct ggtgcctgcg ggatgatgtt
      181 gcagcagagc taaggtcact tagacgctac tatcatttat ccaatggcat ggggtcgaaa
      241 tcagtcgata cccgcagtat atatcgtgaa cttggtgcaa cgctgagtta caacatgcgt
      301 ctggggaacg gcatggaagt tgagccgtgg ctgaaggcgg ctgtgcgcaa agaatttgtc
      361 gatgataacc gggtgaaagt gaataatgac ggtaattttg tcaatgattt gtcgggcaga
      421 cgtggaatat atcaggcagg tattaaagcc tcattcagca gttcgttaag cgggcatctc
      481 ggggtggggt atagccatgg tgccggtgtg gaatccccat ggaacgcggt ggctggtgcg
      541 aactggtcgt tctgaccatc aacgattaaa ctgcgcttcg cgcagttttc gtttaccggg
      601 tgctaaaagg gaaaattctg cggcaaaaaa agcccgccag ttacggcggg aaacctcatc
      661 ctatgggaga acaatgaata atgaaattgc ggggttatca tctcccagta tacccatact
      721 aacaataagg ttatttactc agccaggtat aaacaatttg ttctgtgtgg ggggaacggc
      781 attaagatgt aaaggggggg aagcaatgag tagcatcagc aagaatactc gccgctctta
      841 tcaaacgtgg atgaggggga tgaaaaactc aaggcagaga tgactctgcc ttgaagataa
      901 atgcgctttt acagcgggct tatttcagct cttctgcttc cggtaaggtc acgttcagct
      961 caagaataga aatatcgcca tctttttgct caagctgtac ggttaccatc tcaggatcaa
     1021 tttgtacgta tttacaaatg acctcaagaa tatctttacg caactgcggc agataatgcg
     1081 gttctgcatc actgcgacgg cgttcagcaa caataatctg cagccgttct tttgcaatgt
     1141 tggccgtgtt tttcttccgc gatagaaaga aatcgagtaa tgccataact tatcctccga
     1201 acaagcgttt gaggaagcct ttcttctctt cttcaatgaa gcggaaagga cgttcttctc
     1261 ccaacagacg ttctacggta tctgcgtagg ctttacccgc atcggcgtta atgtcgagaa
     1321 tgaccggttc accctggtta gaggcgcgca atactgattg atcctcaggg atcacgccga
     1381 cgagtttgat gcgcaggatc tccagcacat cttccatgct cagcatgtca cctctgctta
     1441 cgcggcctgg gttatagcgc gttaacagca ggtgctcttt aataggctct tcgccatttt
     1501 ctgcgcggcg tgatttcgac gccagaatgc ctaaaatacg gtcagagtcg cgtactgagg
     1561 agacttccgg gttggtggta ataatggctt cgtctgcaaa atagagtgcc attagcgcac
     1621 cggtctcaat ccctgccggg gagtcgcaaa caataaattc aaagtccatc gctttcagat
     1681 catcaagaac tttggcgacc ccttcacggg tgagggcatc tttatcgcga gtttgcgatg
     1741 ccggaagaat atagagattt tcagtacgct tatctttaat taacgcctga tttagcgttg
     1801 catcgccctg aatgacgttg acgaaatcgt aaacgacccg acgttcacaa cccataatca
     1861 ggtcgagatt acgcaggccg atatcaaaat ctatcacgac agttttcttt cccttctggg
     1921 ccaaaccagt ggcgatggcc gcgctggagg ttgtcttacc aacaccccct ttgcccgaag
     1981 taacaacaat aatgcgtgcc atagaaattc cttgttaaaa agggatcaat ttaacggttg
     2041 aacggtcaaa gcgttttcga ctaactgcag tcgcgccgct ttgccataaa attctgctgg
     2101 gatttgatca ctcagccagt attcacctgc gatggacacc agttccgcca tcaggttcgt
     2161 acaaaatatt tgcgtttccc ggtcaccact tgcccctgcc agcgcacgac cgcgcatcat
     2221 gccatagacg tgaatattac catcggcaat caattcggcc ccagcgctaa cgtggcttgt
     2281 aacaatcaga tcacattgtg gagcataaat acgctgaccg gaacgcaccg gggtatctat
     2341 taaacgcgtt tttgtgaccg gcgttgtatt ttgcgctgga gcctgcggtg cgggagctgg
     2401 acgcggcgct ttttcctttc cttccgtcag gataggcagc cccatctttt caatttcggc
     2461 tttaagttgc gcatctttgc agccacttac gccaataacc cgcaaaccgg ttgccgaaac
     2521 cgccttatgc atcgctgacc agtttaccgg gtcttccagt gcactgacgt tgagtacaac
     2581 gggggcatgt tttaaaaatg cgggggcctg agcgattttg tcttccagcg cctgatggat
     2641 aaccttaggt tctgcctcat gcagatgaac cacagataaa gtgaagctac tgcctttaag
     2701 ctcgattggc gtgtttgaca tcctggcctt actcaattag ctattaatca tcgccagcgc
     2761 gcgatgatgt tccgaagact ataaggcatg ttatagtctg gattatattg aggcaagtca
     2821 ccctcccatt tattcagagt aaaagtctat tctgtgataa atggcgctga ttcatagctt
     2881 aaaaaatacc cttgtcaatc aacccattgc cgtcgtactt ttgattgttc ttatttacgc
     2941 ttcctttttc cgcaccctaa tcgtgacagc atcttcagac ctgtttaaat atccagtatt
     3001 gaggcgagtg tattttttca ccacgccagg ctttcttaac tttccatttt tgcagcatca
     3061 ttaaagaaca gatacccgtt atcttaatat ccgccacaga attgctctat catagaaacc
     3121 gcagaagacg tctgatactt ctgtcaggtc atcgtaactc gtccaggtca gcggttttga
     3181 ttacggattg atgatgaaat gagcataagt gttgtacggt tggattatca ggatgtgcta
     3241 tgaatatgat gcgtattttt tatatcggat tgtcaggtgt ggggatgatg ttctcatcaa
     3301 tggcgtctgg acatgacgct ggcggacttc aatctccggc gtgcggcgtt gtttgcgacc
     3361 cgtatatttg tgttaactca gatggcattt ccccagagtt aacaaggaaa tatctcggcg
     3421 aaaaagccgc tgaaaactta caatcattac aaggctacga tcccagcgaa tttacattcg
     3481 ctaacggtgt attttgcgat gttaaagaaa aattatgtcg tgatgatcgt tattttggtg
     3541 tgaatggtaa gcggagtgga aaaatcaatc aaaccaccac aaagatgtta tttatgtgtc
     3601 gtgagtgatg tcttgtttac attatgttct attcgtcatt aacctcttga gcgacagaga
     3661 ggagtggggc gttaacgttt atgacgggga gagtccccgt cggttgatat ttacttaatt
     3721 tgaatatcga cgttatattt tttagttttg tttttacggg cgtcgtttcg ggtttgcagc
     3781 actcgcaact catatttgcc agaagcaggg agtgagtatt gtccgtgact atcgagttct
     3841 ggggaatatc tggataaatc aacagaatca tcgattcctg gcccgaagag gtaagtatca
     3901 gcgccttcat tagagatact cacatgcact ttctgacctt ttttggcata gaaggtatat
     3961 gtatcgtaat cgtatccctt tatttcgccg gagtactgag cgctgctatg gccttttctg
     4021 aactcaacat tgacattctt acccgcagca aagctcgtgg aggttagcag tgcaagcaac
     4081 aatacagcct tactgatgcc cttaattttc attggtagtc tcctgttcag aatgagtacg
     4141 atgaactgtt ataatataac aattcatcac aggagaattc cagtggcttt cctgcgttcg
     4201 gttaaatccc ggcgcggcat ttttgccggg gggattactt ataaaggggg catggactga
     4261 acaaaccgta ggaatctaca gctggctggc ttgtggcgcg ggtttcattg atgttagctt
     4321 atgccaaaac ctggcatctt aaagagtaaa agtatgtttt gtgtgattta tcgaagcagc
     4381 aagcgtgatc agacctattt atatgtcgaa aaaaaagacg atttttcgcg tgttcctgag
     4441 gaactgatga aaggttttgg tcagcctcag ttagcgatga ttcttccgct ggatgggcgg
     4501 aagaaactgg tcaatgccga tattgaaaag gttaaacagg cattaaccga gcaaggttac
     4561 tatttgcagt taccgccacc acccgaagat ttgctgaagc aacatctttc cgtcatgggg
     4621 cagaaaacag acgacacgaa caaataaccg atatccggcg gtggcattat ctttgtcggc
     4681 gcgggttttc atatccacga taaggtgagg ggaacgttat gtatcaacat cacaactggc
     4741 aaggtgcgct gctggattat ccggtgagta aagtagtctg tgttggtagt aactatgcca
     4801 aacatattaa agagatgggc agcgcagtgc ccgaagagcc agtgctgttt attaaaccag
     4861 aaacggcact gtgcgatctg cggcagccat tggcgatccc atccgatttc ggttcagttc
     4921 atcatgaagt cgaactggcg gtattgattg gcgcgacgct gcgtcaggct acggaagagc
     4981 atgtccgtaa agccattgcc ggttacggcg tggcgctcga tctgacattg cgtgatgttc
     5041 agggaaaaat gaagaaagcc gggcagccgt gggaaaaggc caaagcgttt gataactctt
     5101 gtccgctttc cgggtttatt cccgcggcgg aattcaccgg cgatccgcaa aatacaacgc
     5161 tgggcctgag cgtaaacggc gaacaacgcc agcaaggtac gactgcggac atgatccata
     5221 aaatcgttcc gctgatcgct tatatgagca agttctttac cctcaaggcc ggtgacgttg
     5281 tgctgacagg cacgcctgat ggcgtcggcc cgttgcaaag tggtgatgag ctgacagtca
     5341 ctttcgatgg gcattctttg acaactcgcg ttttgtaata ctttttgccg cctgaaagcg
     5401 gcggcaaaac ttgcatcgct gtgccagact ggttataagg tgcgttttaa cgtaatggcg
     5461 gaacacctga tgagcgatgt acctttctgg caaagtaaaa ccctggacga aatgagcgat
     5521 gcggaatggg agtcgttgtg tgatggttgc ggtcagtgtt gcctgcataa gctgatggat
     5581 gaagacaccg acgaaatcta cttcactaac gtcgcctgtc gccagctcaa tattaaaacc
     5641 tgtcaatgtc ggaactacga acgtcgtttc gagtttgaac ctgactgcat taaattaacc
     5701 cgtgaaaatc tgccaacatt cgaatggctg ccaatgacct gcgcttatcg cttgctggcg
     5761 gaaggtaaag atttacctgc gtggcatccg ctgcttactg gttcgaaagc ggcaatgcat
     5821 ggtgaacgta tctctgtgcg tcatatcgca gtgaaagaat cagaagtgat tgactggcag
     5881 gatcatatct taaataaacc tgactgggcg cagtgataaa tttaatagat gttgatgggg
     5941 gctttcttaa tatacgtctg gatactcgcc atttcgtagc cctgtttttg aaagcgctct
     6001 gtcataatga gagttacccg ataccttatt atcaacaggt aactctcaat ctttaaaaaa
     6061 cggctgtgtt tttaaatgtg ataaacctta taccttgagt acatggagag aataatcgct
     6121 tatcagactt caggtacctc aaagagtgtc tttttaccgt gtcttttctg atactcatta
     6181 caggtgttaa tcattttttt ggccgcttct tttagcaaag aaagcattaa atcatcataa
     6241 tcaacataga atctggttgt ttcagtttcc gtttttatct ccccgatggc ggctatttcg
     6301 gtggttaatt tcaatttggc ggcatcgata tctttattcg cttgtttaac cgtgttagac
     6361 agggtggtaa agaaactctg cacagatttt aacttgttct tcaattctgg aatcagtttc
     6421 ccttcaacta cgcccgctgc aacagaatac agtttcttga tgccgtcatc cagtacctta
     6481 atgagaatgc ctttctgggc gaatgcttct attctatttt ttagccgggc gatatttttc
     6541 atttctgctg gatgagcgtc gtcgccagaa ggccacgtga gcacaggata agagaacgaa
     6601 aaatcagcag tctatgcagc gacaaatatt gatagcctga atcagtattg atctgctggc
     6661 aagaacagac tactgtatat aaaaacagta taacttcagg cagattatta tgttgtttat
     6721 caagcctgcg gatctccgcg aaattgtgac ttttccgcta tttagcgatc ttgttcagtg
     6781 tggctttcct tcaccggcag cagattacgt tgaacagcgc atcgatctga atcaactgtt
     6841 gatccagcat cccagcgcga cttacttcgt caaagcaagt ggtgattcta tgattgatgg
     6901 tggaattagt gacggtgatt tactgattgt cgatagcgct attaccgcca gccatggtga
     6961 tattgtcatc gctgctgttg acggcgagtt tacggtgaaa aaattgcaac tacgcccgac
     7021 agtgcagctt attcccatga acagtgcgta ctcgcccatt accatcagta gcgaagatac
     7081 gctggatgtc tttggtgtgg tgatccacgt cgttaaggcg atgcgctgat gtttgccctc
     7141 tgtgatgtaa acgcgtttta tgccagctgt gagacggtgt ttcgccctga tttatggggt
     7201 aaaccggtgg ttgtgctatc gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca
     7261 aaggcgctgg gcgttaaaat gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc
     7321 tgtggcgtgg tttgctttag cagcaattat gagctttacg cagacatgag taatcgggtg
     7381 atgtcgacgc tggaagaact atcgccccgc gtcgagattt acagtattga tgaggcattc
     7441 tgcgatctga ccggtgtgca taattgtcgc gatctgactg attttggcag agaaattcgc
     7501 gcaacggtgc tacaacgtac ccatcttact gttggtgtgg ggatcgccca gaccaaaacg
     7561 ctggctaagc ttgccaatca tgcggcaaaa aaatggcagc ggcagacggg cggggtggtg
     7621 gatttatcaa atctggaacg ccagcgtaaa ttaatgtctg ctcttcccgt ggatgaagtc
     7681 tgggggattg gacggcggat cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc
     7741 gatttggcgg attcagatat ccggtttatc cgtaaacatt ttaatgtcgt gctcgaaaga
     7801 acggtgcgtg aactgcgcgg tgaaccctgt ttgcaactgg aagagtttgc accgacgaag
     7861 caggaaatta tctgttcccg ctcgtttggt gaacgcatca cggattatcc gtcgatgcgg
     7921 caggccattt gtagttacgc tgcccgggcg gcggaaaaac ttcgcagcga gcatcaatat
     7981 tgtcggttta tctccacgtt tattaagacg tcaccatttg cgttcaatga accttattac
     8041 ggcaatagcg catcggtaaa actgctgacg cccactcagg acagcaggga tatcattaac
     8101 gccgctacgc gatctctgga tgccatctgg caagcgggcc atcgctacca aaaagcgggc
     8161 gtgatgctgg gggatttctt cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac
     8221 gcaccgcgcc ccgggagtga gcaattgatg gcggtaatgg atacgctgaa tgctaaagag
     8281 ggcagaggaa cactctattt tgccgggcag gggatccagc aacaatggca gatgaagcgt
     8341 gccatgcttt caccatgtta tacaacgcga agttctgatt tactgcgggt caaataaaca
     8401 tagcggcagg aaaaagcgct cccgcaggag cgccgaatgg attagcgacc gaacaggtca
     8461 cgttttttcg ctttaaacgg ctgggaaatc accaccagca ccgcgacaat caggtaagcg
     8521 ataaaaatac cgagcagcca ctgcggcatt tccagaccta aaaattccca ctgacgctcg
     8581 gcgcaatcgc cagaggcgac aaacacttgc ggcacccatt tatccagcgg cagccattcc
     8641 gggaaacgag ccataaaatc acaggtcgca aacggcgaag gatagagctg aagcatggtg
     8701 tgctcgtaag ttaactgcac accgcggaac gcactataca accagataac catcgctaca
     8761 taacgcagcg gagttttcgg ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat
     8821 aatgcgcagc gttcataaat acagagcacg caaggtttca gtaacatcac atgctggaac
     8881 cacagtgccg tcagttccag tgctagagcg gtaaacgcca tcaacagcca cgcgccccgg
     8941 ccttgtgaac attggttcaa aaatcgcaac ataatcattt ccctgcaata tgcatagagt
     9001 gcgcagttta aaccaattca ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat
     9061 tggacaatta tcgggcgaaa aggcaaaccg ggcatttata tgcccggtaa gttgttatca
     9121 aagcgttgct atccagccca tttgcataaa ccattcggtt acaggggcaa gcgtaaactc
     9181 gacgcagagc aagccgacga gtgtcaggac gagggtgtaa ggcagggcca tccacaccat
     9241 gcggccataa gagaggcgaa tcaatggcgc gagtgcagag gtcagcagga acaggaacgc
     9301 agcctgaccg ttcggcgtag cgacggaggg cagattggta ccggtattaa tggcgaccgc
     9361 cagcagctcg tattgcttca acgtgatagc gccactttcc attgccgctt tcgcttcgtt
     9421 gatataaatc gtccccacga agacgttatc cgaaatggat gacagcagac cgttgaaaat
     9481 atagaacagc gacagctgag catgttccga tgcctgcaac acaaactgaa taatcggcga
     9541 aaacagttgt tggtcgataa tcaccgcgac taccgagaaa aacaccgtca acagtgcggt
     9601 gaatggcaga gactcggtga aggctttacc gatagcatgt tcatcggtga caccggtcaa
     9661 tgatgtcgcc agaataatga ccgacaaacc aatcaagcca acttccgcca gatgcaacgc
     9721 cagcgcagtc accagccaga cgccaataat cgcctggaca atcagacgga ttttatcctg
     9781 acgggtacgc tggtggcggc tttgatcgtc aaactgttgc agtacttcgc ggactttctc
     9841 cggcagcgtt tcaccatagc caaaccaacg cagcttctct accagcaggc aggttaacag
     9901 gccacaaatc agaaccggaa cggtcactgg cgacatgcgc aggaagaaat cgccaaaatg
     9961 ccagccagcc gctttagcga tgatcaggtt ctgtggttcg cccaccatgg tcatcacgcc
    10021 gcctaatgcg gtgccgacac cggcatgcat catcaggcta cgcagaaagc cacggaactg
    10081 ttccagaacc actttgtaat gcttgtcgat atgactatcg tcttgcaggt cggtatcttc
    10141 ggtacgggaa gaggctacgc ggtgataaat accataaaaa ccgactgcaa cgctgatcac
    10201 caccgccacg acggttaagg catcgaggaa cgcggagagg aacgccgccg ccacgcaaaa
    10261 agagagcgac agcagcattt tggagcgaat gcttaacagc aaacgggtaa atatgaacag
    10321 caatagctgt ttcataaaat agatacccgc caccataaac atcagtaaca gcaagacttc
    10381 aagatttgcc gccacctctt cacggacgtg ttccgcgctg gtcatgccga tgaataccgc
    10441 ttcgatagcc aacagaccac cggggagcag cgggtagcat ttcagggcca tcgccagagt
    10501 gaaaataaat tccgctacca gcaaccagcc agcgacgaaa gggctgatga ggaaaattaa
    10561 cggatttacg attaagaaaa tgatgagggc gagtttgtac cagtcggggg actggcccaa
    10621 aaaattgcgc catagtgcgc ggccccagga gatctccatg atggtttccc ttaccttaga
    10681 aataatcaat gatgttttta tgtttaaacg caaagcttaa cggtcagaca ggagtgaggc
    10741 aagtcttgat agtcaagggg aaagagatgc agaaaatgaa gccttgatcc cttttttctt
    10801 ctttttgtct tctatcagca tagttagccc tctggtatga tgagtccaac tttgttttgc
    10861 tgtgttatgg aaatctcact atggtcatta aggcgcaaag cccggcgggt ttcgcggaag
    10921 agtacattat tgaaagtatc tggaataacc gcttccctcc cgggactatt ttgcccgcag
    10981 aacgtgaact ttcagaatta attggcgtaa cgcgtactac gttacgtgaa gtgttacaac
    11041 gtctggcacg agatggctgg ttgaccattc aacacggcaa gccgacgaag gtgaataatt
    11101 tctgggaaac ctctggttta aatatccttg aaacactggc gcgactggac cacgaaagtg
    11161 tgccgcagct tattgataat ttgctgtcgg tgcgtaccaa tatttcaact atttttattc
    11221 gcaccgcgtt tcgtcaacat cccgataaag cgcaggaagt gctggcaacc gctaatgaag
    11281 tggccgatca cgccgacgcc tttgccgagc tggattacaa cattttccgc ggcctggcgt
    11341 ttgcttccgg caacccgatt tacggtctga tccttaacgg gatgaaaggg ctgtatacgc
    11401 gcattggtcg tcactatttc gccaatccgg aagcgcgcag tctggcgctg ggcttctatc
    11461 acaaactgtc ggcgttgtgc agtgaaggcg cacacgatca ggtatacgaa acagtgcgtc
    11521 gctatgggca tgagagtggc gagatttggc accggatgca gaaaaatctg ccgggtgatt
    11581 tagccattca gggacgataa tcccttccgt ttaaaaagca aacccctcaa acgaggggtt
    11641 tttcgttgtt tttacagatt tcccattctc ggcgggcaac gttccagcaa ctcgacgctg
    11701 ccgtcttcgt tttgctgttc cagcatcaca tcaaatcccc acaggcgatg cacatgcttc
    11761 agcacttctt tgcgcccccg atccagcggt gcgcgattat gtggaatgta acgcagcgtc
    11821 agcgaacggt cgccgcgcaa atccacgttc cagatctgaa tattcggctc cagattactt
    11881 aaattatatt gagacgataa ccggttacgg atctcccgat aaccttcttc attatgaata
    11941 gcggaaatct ccagataatt atgccgatcg tcatccagca cggtgaagaa gcggaaatca
    12001 cgcatcactt tcggtgacag gaactggctg ataaaactct catctttgaa atcacgcatc
    12061 gcgaaatgca gcgtttccag ccagtcggaa ccggcgatat ccgggaacca gtatttgtct
    12121 tcttccgttg gcgactgaca aatccgttta atatcctgga acatggcgaa cccgagggca
    12181 tatgggttga tgccgctgta ccacgggctg ttatagggcg gttggaagac cacattggtg
    12241 tggctgtgca aaaactccag cataaaacgt tccgttactt tcccttcatc atacagatgg
    12301 ttaaggatgg tgtagtgcca gaaggtcgcc cagccttcgt tcatcacctg agtctgtttt
    12361 tgcggataaa aatactggct caccttacgc acaatacgca ggatttcacg ctgccatgat
    12421 tccagcagtg gcgcattttt ctccataaaa tagagcaggt tttcttgtgg ttcggacgga
    12481 tagcggcgcg cttcagcaac cgttttctct tcctcgcgct tcggcagggt acgccagagc
    12541 atattgacct gactttgcag atactcttcg cgactcttct gccgggcttt ctcttcttgc
    12601 agcgagattt tttgcgggcg tttgtaccgg tccacgccgt agctcatcag cgcgtggcac
    12661 gagtccagaa gccgttctac ttcatcaacg ccataacgtt cttcgcactc ggtaatatat
    12721 ttacgggcaa aaatcagata atcgacaatc gaactggcgt cggtccagct acggaataag
    12781 taattgtttt tgaagaaaga gttatgccca tagcaggcat gagccatcac cagcgcttgc
    12841 atggtaatgg tgttctcttc catcaggtaa gcgatacacg ggttagagtt aatgacgatt
    12901 tcataggcca gtccttgctg accgtgctta tacagccgtt cagtctcgat aaacttttta
    12961 ccgaatgacc agtgcggata gttaattggc atgccgacgc tggagtaggc atccatcatc
    13021 tgttctgagg ttatcacttc aatctggtgc gggtaggtat ccagccggta gagtttcgcc
    13081 acccggtcta tctctgccag ataaacatcc agcaggtcga acgtccagtc gggtccatcg
    13141 ctcaaacgtg tggtgtcctt attcatagaa tcgatcgtcg ccatacgcgc acctcattgt
    13201 tgtcggcgct ctctgtgtgg agcacctcat ttcaagcata gaacacctgt taaaaaccgc
    13261 gtcgccggag aatttttttc tttgcgattt cttattatca gagtgccact aatccgcttc
    13321 tgaacggaat tttatgctgg ataaaaaggg cgttcagcag gagatactaa agacgccata
    13381 ttgccgcaga gtcagggaga tgtgagccag ctcaccataa aaaagccgta tgttgaataa
    13441 tattttcaac tgagttatca agatgtaatt agattattat tcttttactg tatctaccgt
    13501 tatcggagtg gctatgcgag ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc
    13561 ctggtactta aatcaggcag gacatgaggt caccgtcatt gatcgggagc cgggggccgc
    13621 cctggaaacc agtgccgcga atgccgggca aatctccccc ggatatgctg caccgtgggc
    13681 ggcaccaggt gtgcctttaa aagcgattaa atggatgttc cagcgccatg cgccgctggc
    13741 ggttcgtctc gacggtacgc agttccagtt gaaatggatg tggcaaatgt tacgtaactg
    13801 cgacaccagc cactacatgg aaaacaaagg gcggatggta cgtctggcgg aatacagccg
    13861 tgattgcctg aaagcattgc gcgccgaaac caatattcag tatgaagggc gtcagggtgg
    13921 gacgctgcaa ctgttccgta ccgaacaaca gtatgaaaac gcgacccgcg atatcgccgt
    13981 gctggaagat gccggcgtac cgtatcagct gctggaatcc agccgcctgg cggaagtgga
    14041 gcccgcgctg gcagaagtgg cgcacaaact gacgggcggc ctgcaactgc ccaatgatga
    14101 aaccggagac tgccagctgt ttacccagaa tctggcgcgg atggcggagc aggcgggggt
    14161 taaattccgc tttaatacgc ccgttgacca actgctttgc gacggcgagc aaatctacgg
    14221 cgtgaagtgt ggtgatgaag tgattaaggc cgatgcgtat gtgatggcgt ttggttctta
    14281 ctcgacggcg atgctcaaag gcattgttga tattccggtg tatccgctga aaggctactc
    14341 gctgaccatt ccaattgcgc aggaagatgg tgcgccggta tccactattc ttgatgaaac
    14401 ctacaaaatc gccattaccc gtttcgataa ccgcattcgt gtcggcggaa tggcggaaat
    14461 tgttggtttt aacaccgagc tgttgcaacc gcgtcgtgaa acgctggaga tggtggttcg
    14521 cgatctctat ccacgcggcg gtcatgttga gcaggcgact ttctggactg gcctgcgccc
    14581 gatgacgcca gacggcacgc cggttgtcgg gcgtacacgc tttaaaaatc tgtggctgaa
    14641 taccggtcac ggcacgctcg gctggacgat ggcttgcggt tccggtcagt tgttaagcga
    14701 tctgctctcg gggcgcacgc cagcgatccc gtatgaagat ctaagcgtag cgcgctacag
    14761 ccgtggattt acgccatcac gcccgggcca tttacatggc gcacacagct aaggaaacga
    14821 gatgacccgt ccgatacagg ccagcctcga tctgcaggca ttaaaacaga atctgtccat
    14881 tgtccgccag gctgcgccgt acgcgcgcgt ctggtcggtg gtaaaagcaa acgcttacgg
    14941 gcacggtatt gagcgtatct ggagcgcgct cggggccacc gatggctttg cattgcttaa
    15001 tctggaagag gcaataacgt tacgtgagcg cggctggaaa ggaccgatcc tgatgctgga
    15061 aggatttttc catgctcagg atctggagat ttatgaccag caccgcctga ccacctgcgt
    15121 acacagcaac tggcagctca aagcactgca aaatgcgcgg ctaaaagcac cgttggatat
    15181 ttatcttaaa gtgaacagtg ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac
    15241 cgtctggcag cagttgcggg cgatggcgaa tgttggcgaa atgaccctga tgtcgcattt
    15301 tgccgaggcg gaacatcctg atggaatttc cagcgcgatg gcgcgtattg agcaggcggc
    15361 ggaagggctg gagtgtcggc gttcgttgtc caattcggcg gcgactctgt ggcacccgga
    15421 agcgcatttt gactgggttc ggcctggcat tattttgtat ggcgcttcgc cgtccggtca
    15481 gtggcgtgat atcgccaata ccggattacg tccggtgatg acgctaagca gtgagattat
    15541 tggtgtccag acgctaaaag cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg
    15601 cgatgaacag cgaatcggca ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc
    15661 gcctaccggt actcctgttt tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc
    15721 gatggatatg ctggcggttg atttaacgcc ttgcccgcag gcggggattg gtacgccggt
    15781 tgagctgtgg ggcaaggaga tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg
    15841 ctatgagttg atgtgcgcgc tggcgttacg tgtcccggtt gtgacagtgt aacttgttat
    15901 cgctggatgc gaccagcgtc gtatccagcg ttacaaaaca cattaaactt aagattcggc
    15961 ttcttcctcc gctacccgaa caccaatctt caacacttca ttatcctctt tctcggccac
    16021 cgtccagatc attccggcaa attctacctg atcaccgaca accggtgccg cgcctaacaa
    16081 ctgctggaca atttcaccca gcgtttgctg cttatcacga tattctcgcc cgtcttccag
    16141 gccatatatc agcgccacat cagcatattt ggcactggct tcgagaatga agtcaccaaa
    16201 gaagcgttga tctagcgcga ccggtggcga ctggctgaac agtttaccga gcgccgggag
    16261 atcgcgttcc cgaccaatta cacacaacac atcgccttcg cgcagtcggg tgctgccggt
    16321 aggatgaagc aactggttat cacgaaacag tgccgcaata cgcgtctctt ttggcatatg
    16381 cagatcgcgc agtgccgcgc ccacgcacca tttatcggca ctcaactgat aaacaaactg
    16441 ctcccacgga ttttccggat gaatatccag accaacgcgt gacaccggac gtcccactgg
    16501 cggaacgacc actttggctt ttttcgccgc ccacgagagt gatgtcccct gcaagagtag
    16561 tgaaaccaga accacaaaga aggcgacatt aaagaacagt cgcgcattct ccagccccgc
    16621 catcatcggg aacacggcca ggatgattgg caccgcgccg cgtaatccca cccagctgat
    16681 aaacacgcgc tcacgcagat tgaagccacg gaacggtagc aatccggcaa ataccgaaag
    16741 aggacgggcg aagaaaatca tccatgcgga caaaatgagc gccggaatgg caataggcag
    16801 cagatcgctt gggttaacca acagccccag caccaggaac atggcgattt gcgccagcca
    16861 ggcgaggccg tcgaaatttt gcagaatgcc gtagcggttg cgaatcggcc gattacccag
    16921 cagaaaaccg cacagataaa cagccagaat accgctgcct tccagcgccg tggttaaagc
    16981 aaaaattaaa ataccgccgc ttaatgccag caatggatat aaaccggcgg gcagggcgat
    17041 gcgattaatc atttgcagca gtaaataacc accgccaaga ccgatgacaa tcccgaggcc
    17101 aaattgctgc aaaatatcga ccacgaacat ccagctgaca ctgctttcat gctgctggat
    17161 catcgcaatc agggtaatcg tcagaaagac cgccattgga tcattactgc cggattcaat
    17221 ttccagcgtc gagccaacac gttcgttaag ccccttacca cccagcagag aaaagaccgc
    17281 tgcagcatcg gtggagccga cgatagcgcc gattaataat ccttcaatca aatcaagatt
    17341 aaacagccac gccgccatca tgccggttaa accagaggtg ataagcacgc ccagcgtcgc
    17401 cagcgacagt gccggtccta acgccacacg aaaggaactg gcctgagtgc gcatcccgcc
    17461 gtcgagcaaa ataatcgcca gcgccagatt acttaccatg taggcgaagg gataattatc
    17521 aaacgggata ccgccgacgc catcaacccc cgccagcatg ccgatcgcca gaaaaataac
    17581 cagaatggga atgccaagac gggaagaaaa tgaactaagt aaaatactgc tggtgacaag
    17641 gatggatcct aagatgaaaa ggctaattat tgttgtggca tccaacggtc ggttactcct
    17701 gattacgctg tctcttatat aaaccctacc atattagcgg cagagacagc gttttactta
    17761 gccctgaagc gtgatttttt tacattttaa gaacaggatg accgctaatc gtcaactggg
    17821 tgccttcctg ggtgttattc agaatggcat gtgcgcccaa cggcagtgta accgtgcgtt
    17881 gttcatgacc aaaatcgagg ccggtaatga gtggaatcga caggcgggaa cgcaaaaacg
    17941 cgtacactga ctccaggttg taacctgcgt cataatcatt aggcgtgctg ccgctaaagc
    18001 taccgagaat aatcgccttc tgacgtggca aaattccagc atgatagagc tgcaacagca
    18061 tacgttcgac gcggaaaggg tgctcgttaa tatcttccag caccagaata ccgttctcaa
    18121 tttttggcat ccacggtgta ccaatcagtg aaatcagcat cgcgagattg cctccccaca
    18181 gcgtgccttc ggtctgacat gtcgggcctt ccccttgcca ttcaatggtg aaggtttcat
    18241 tgcgtaacgc cagccagaaa tggtgctcgg taaaggcgtt cagttcatcc gcgccaaagt
    18301 ttgctaccag catggggccg ctaaaggtga tgacattgcc atgcgccaga agaccgcact
    18361 gaatggcggt aaaatcgcta tgtccgcaaa tgagcaacgg gtcatgttgt tggcgagcca
    18421 ccagtgcctg ccagtcaata tctgccagta aacgactggc accgtaaccg ccgcgtacag
    18481 ccagcacgat ggtgttgggg gtagtcagtc tggcaagaga attgagatct tccagacgct
    18541 ccgtttccgt acccgcaaaa cgctcacaac gacgggcaat aacctcgacg ttattgacct
    18601 gatgccccgc gtcggtaagg cgctggatac cacgcagcgc ggcgtgctgt ttaatgcagt
    18661 aacccgatgg ggcaattaag tgaaacagag acatggcaat tccttgctga caacagaaac
    18721 gaaatgtata tcatgccgct taggtgtgcc gttgtcacct caacggcgat tccaggctat
    18781 aaggatagaa gaagtgaaat tgagatggtt tgcctttttg attgtgttat tagcgggttg
    18841 ttcatcgaag catgactata cgaacccgcc gtggaacgcg aaagttccgg tgcaacgtgc
    18901 gatgcagtgg atgccaataa gccagaaagc cggtgcagcc tggggcgtcg atccacaatt
    18961 gatcacggcg attatcgcta tcgaatcggg tggtaatccg aacgcggtga gtaaatcgaa
    19021 tgccattggt ttgatgcagt taaaagcttc aacctccgga cgtgatgttt atcgccgtat
    19081 gggctggagt ggtgagccga cgaccagcga gctgaaaaat ccggagcgta atatttcaat
    19141 gggggcggct tacctgaata ttctggaaac cggcccgctg gcaggcattg aagatccgaa
    19201 ggtactgcaa tatgcgctgg tggtgtcata tgctaacggg gcaggtgcgc tgctacggac
    19261 tttctcgtca gatcggaaaa aggcgatcag caaaatcaac gatctggatg ctgacgagtt
    19321 cctcgatcac gtagcgcgaa atcatcctgc gccgcaggct ccgcgctata tctacaaact
    19381 tgagcaggca ctggacgcga tgtaaatcag tcgcgcactt tgtccgcttt ctcccgggct
    19441 tctcgctcga gagagaaaat aatccgctgt aattgccgct ccaccgtcgg gctgacgtta
    19501 agaaaacgga agctcagacg gggagtggtg atggtttcat tcttgccatc aatcactttg
    19561 cgctcgctga tggagattaa ctgggcgtca aagtgaaaaa caccccattg ccccatgttg
    19621 acttcaatct gagcgaagcg catgccttcg tgcaatccgg caggctttgc tgtttccagt
    19681 aatgcgccca tgccgcctaa cgacaaatca tacaggcgga aacgtaacgt actgttatcc
    19741 gccagtttgg tctggcaaaa atatggcgga tggagtgggg cggagatgcg gaaatatcgg
    19801 cgtcgttgta caaaccacaa ggtgggaggc ggtacggtaa taaatgccgg aagctgcaag
    19861 tattcactct gctgtagttg ttcaacagta aactcgactt tcgcaccctg ggtttcggcg
    19921 gtaatggtaa tgtgctgcgc ctttaacaca gcgttgttat cttcggcttg actgccgaaa
    19981 tccagcacca gtttatccgg ggttattgcc agtattttgc tgatcagctg cccgccattc
    20041 caactgatac gcaaaggaat tgcggctttg tgcaaatcgc gtaacacgcc caggacggct
    20101 aacggatttt gtttcaggaa ctgctcatgg taatgactca cgcggaaaaa ctcctgactg
    20161 acaaactgtc ttaggtttat cggtcacagt taacaaaact taatacaaat gcgtgaatat
    20221 tttttacatg tcgttcttaa atcagccgac atacgcccag catcgatccc gcccttgcct
    20281 atacttagag cgttgacgta agcatttctt gcgtccgatt catcgaaacg agggcctgaa
    20341 catgggaatt attgcctgga ttatttttgg cctgatagcc ggcattatcg ccaagctaat
    20401 catgccgggg cgtgatggtg gtggattttt cctgacctgt attcttggga tagtcggtgc
    20461 ggtggtcggc ggctggctgg cgaccatgtt tggcattggc ggctccatca gcggttttaa
    20521 tttgcacagc ttcctggtgg cggtagtggg cgctatcctg gtgctgggcg tattccgcct
    20581 cctgcgaaga gaataagatt ttcataaggc ggatagcgat acagatgccg ctatccgctt
    20641 tcacatcaga acgtatactc gatacctgcc cagaaattgc gaccttcttc aataaaccct
    20701 tcactgtagg catatttggt atcaaacagg ttattgaccg acgcattgac gctgaagcca
    20761 tgacctaagg tgtaatcggc tcgaatgtgg gtcaccgcaa aaccggcggc tttttgtgaa
    20821 ccgtcactgt tgctgtagct ggaggaacgc gcctcttccg acagcgttac gcttaacggc
    20881 tcccacggtt tgagagtcat ccatgcggtc attgtctgcg ttggcagatc ggttatcttg
    20941 ccgatgtctt tgcgtttggc atcagcgtgg atcagggcgt agctcaatcc tacatccagt
    21001 atattgctga ttttcccctt aataccggca tccagaccgc tgtaatccac cgtgccgctg
    21061 ttctgatttt gaatggtatc ggcatcgata ttgtgcgaga ggatggcatc actcacccgg
    21121 ttatagtaaa cgctgacctc aaacccccag tcgtgcgtga aggcaccatt ccaggttaaa
    21181 tccaccccgc gcgcgcgttc cggtttgagc tgcgggttaa caatcgctat ctggttatac
    21241 gcaggtttgg acgtggtata gcgttctttc agcgtcggaa agcgtgtgcg gtcatagtac
    21301 gaaagcgcca gcgtgtcttc attggcaaag tggtatttcc ccatcacctg ccagttaaaa
    21361 gctgactgat tgttgtcgtc ataatgggtg atgcttccat ctttctcatg ttttttcgct
    21421 tctacgctat cgcgccagtc atagctgatt ccagccacga catcgacatt atcggcagca
    21481 gcccattgat attcactggc gagcgaccag gtacgatctt catagcgatc gtaagcggcg
    21541 tgcggcgcac ctttttcccg gtgtacgtca tctttccagt taacggcaaa cgacagcaga
    21601 tcgttttcac gcacatcggc tgccagttgt aacccggcac cgtcgctgta atcggaataa
    21661 tggctgtagc tgcctttttt atttttcaaa tcagccagcg agttgtacat catcagcgta
    21721 ttttcaaagg tatcgcgata cagccgactt ttcagggtaa aacgatcgtt tagttgggtc
    21781 gttccctgat aataaaaact ttctttgtca tactctggcc actgccagta gcgtgatttt
    21841 tgaccactat ttccactgta tggcggatta tctttttcac catcctgctt aatgtaagtc
    21901 aatgtgtatt catcgttttc gcgtggtgta aaaccgagct tcacaatgcc gcgtttatca
    21961 tcagccgatg aattaatcat cttgccgtgt ttgcctgcaa tatcattatt tacaccatgc
    22021 ggcaggccga gaaaatcctg ctttagctgg ctaccgctga cttgcaaata ccccagatcg
    22081 ctgctggcgg caaatgaagc atgcatatcg taggcattgt cctggctacg gctccatccc
    22141 tggcgatatc ccagatttgc ttccagaggt tttgttggct tctgggtggt gatattaatg
    22201 gctccgccca tctgattagg cccctgaagc agcgacgaat accctttgga aacttcaact
    22261 gcccccaaat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata gggaacataa
    22321 atgggcacac cgtcgaaata gactggcacc tgacgactat caaagccacg aactttgacc
    22381 tgttcttcgt tgcggctacc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    22441 tgagcgacat tctgtttatc cagggccttc atggtggggt gatcgagaac ggtggtcgtc
    22501 gtcgatgata ccggactgga ccatacggtc agcgtgtcac tctcctgagc cgctaaggcc
    22561 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    22621 taaattccgt ttaacaaaga attaaataga ggctgcgggt tgcagactga ctttaatgtc
    22681 agctggtgtc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    22741 atttttgagt gttgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    22801 cgacggtgct atttgcgcaa tttcagttgc ctgttgcgga ttaaatttgt cgagctgaag
    22861 tacgtctggt tgcgcgcgta acgcggcgat tgcctctttc ggcgtgtcgg cttcaacaac
    22921 tattttcttc tcgggtgcgt ggcggcgtaa ctgattgatt gcgcctgacc agtcctgatt
    22981 gtcatgaaga aaatgacggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    23041 atgaatcagt cctccggcag ccagaattgc ctgcgaggcc agtaagcggg tgcccggaat
    23101 tgcttttcgg gtgcaggcga tattgccatc agggtaacgt tcacgaagta acgccagcat
    23161 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    23221 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgctgac ccgcgttcgc
    23281 ttgtgaaccg tcgctgactg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    23341 cgcgacagaa attccgctga cgcagccgcc ctgacggtga aaaaactcta tatagccatg
    23401 ctggtgtcca atatttaatg ctcgcgtggt caggtcaccg ccctggatat cttccagaag
    23461 taacgcatcg atttgtgcct gagaaaggaa aatcatcgca gtgcctcttg cggtacgcta
    23521 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    23581 tgataccagc gatataaccg ctcacgctca tcgtcgttaa gcgcgcccag actccagctg
    23641 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccgcggata
    23701 aaatcaacgt gagcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    23761 ggtaattcga ccacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    23821 ctgaccagat gagtcgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgcc
    23881 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    23941 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtctgc ctttagcgcg
    24001 gcggcgcgac gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaatcgtc
    24061 gtgagtttgt ccgccagcgc cagcgatacg gtacccggtc cgcagcccat atcgaataag
    24121 gtttgcgcgc cttgcaggtc aatttttgag gtcagttgtt gcaggtagct gtccgtcggc
    24181 gaggcgcaat tttccgccat tttttccgcc cgttgatccc agtgatccgg cgttttttct
    24241 gtgcgatggg ccagcctgag ttgctgaagg taaaggtctg caaaatcaat atcgtcaatg
    24301 agcatgattt ttccttagtg cttaattgca gagaggtgat ggtgaatctg gtcggcagaa
    24361 acccggtaca gggcagccag ttttttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    24421 actcgtccat ctggctctac ctggataatg ctgtcggcaa tggcgtttgc atggagcgga
    24481 tgatgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    24541 agttggatct gatgaccgaa atcgaggcta gaggcaggct catccagtaa taacaagcgc
    24601 ggttgctgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    24661 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgttgctttc
    24721 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggcctg gcccatcaac
    24781 accatatcca gcacactaaa agcaaaaatg ccgtcgtgcg cctgcggcac ccaggcaata
    24841 gcctgcgcgc gctggcgatg gctgagtgtc tgcacaggga ggctatccag taagatctcg
    24901 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    24961 ttggctccta acagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    25021 accggatggc gggagtaacc cagagacgca tgacggacgg caagcaaagt cactggcgac
    25081 ctccgcgtaa caacaaaaac aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    25141 gtgggatttc tgttgttcca atgctgcgtg ccagcgtatc ggtaagcagg agcagaatcg
    25201 cgccagtaca cattgccatg ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    25261 caatgtgcgg aacgaccagg ccgacccaac caattatgcc cgctattgct accgtgctgg
    25321 cggtgatgag tgtggcacag acaatcaatc caaagcgcag acgtgtcacg ttcagtccca
    25381 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    25441 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    25501 tggacagccc ccccagcaac cagaaggtaa ttgacggtaa ctgggtataa ggatcggcca
    25561 gcgtttttat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    25621 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    25681 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatggat aatccccaca
    25741 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaca
    25801 ctaagggatt acggaacatt ccctgatagg tggttccggc ccctgccagc gccgcgccca
    25861 gaaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    25921 cagtaacatt ttcttgacga agaatgagcg ccagcagttg ctgcatatcg agatgataag
    25981 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    26041 cggttctcat tagctcgcca ccaatttttg atagtcggcg tcacttaatg ggcaatgcca
    26101 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    26161 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catccagcca
    26221 gccaaagggc aggccactta aaaataagat gcgttgttcc gcaaccgctt tcacgccctg
    26281 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    26341 cggctgccag cgcaggagat tttccatgga aacctgtgtc agaccgtggc gatcggctat
    26401 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    26461 gcccgtttcc agccctcgag gaccgcgcgc agcataaaag ctgagattag cggcgggtga
    26521 ggtggcgaat gcctgagctt caccaacgaa gcggctggcg agattggctt gttctgcggc
    26581 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgttccag
    26641 tttcccgtta agcaataacc agggtatttg tgtctgttcg ctaacctgcc gtgcctggga
    26701 gatccaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    26761 cccttccagc gagagtgtac tggcgcgccc ggcgagtcgt cccagcctgg ggaactggcg
    26821 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    26881 cattttttct ggcgcgacag ccagcaatag taaatcggcg ggcgcgcctg cgctgacgat
    26941 gcgtgtaatg ttttgggctt cgggaagcgt cagtgaggcc cagaatttga ctgattgtgc
    27001 aaaggacggc agatgatgca ggaacaatgt cgacgcaaga atctgtgtaa acctccgtct
    27061 ggttagtgac ataagtaata tcctgataat taaaaccgct atatacttac acatatagcg
    27121 aaaaagatca tgtttgatgt caaaatttgt ctaaagagac ggaatgattt gcttgttgct
    27181 taaataacac tttgttccac aagaaaattg tcagggagaa tggggagtgg gggaaaatat
    27241 aaatagaggt gtaatgatta acaagttgat aaaataaaat taaaatcttc tctaataaaa
    27301 gaaaataaag gcatagaccg tagaaagagt ggtatttggt atttcgtggt gaagaaagag
    27361 caatgccaat tttatgcacg taatggcatt gctcgatata cagtaaagtt aaggcgtagg
    27421 ttccgcctca ttttgtttta ccggctgtgt tgttgattca ctgagtggac gcgtcgccgg
    27481 aacattgtcg cacggttgct ctttcgggca gatcaaatcc agcattttca gcgtcacgcc
    27541 attggtccag ccaaagccat cctgtaatgg atattcgcca ccgccgcccc ctgttccggt
    27601 ggtgctgaca tcatattttt ccaccagctt tttctcccgg tcataggtgt gctgaacatt
    27661 ggtcaggaag tgccagctaa tgtccatcgc cacctctttt tgcccgtagt tttgtaatcc
    27721 ttctgtcgcg acccactgca acggtgccca gccgtttggc gcatcccatt gttgcccact
    27781 tttcaccgac gtggtgttca ggccgcctgg ttgcagcaga tgtgttttcg tcgccgtcgc
    27841 cattttgctg gcgcgatctt tcgctgccgc attgacgtac agcgggaaca gggcggccgc
    27901 ggttaactga ttgcgcactt tatgactttt caggtcgtaa tcggcatacc agccttgttg
    27961 atcgttccac aggtattttt cgatcccttt ttgacgggca ttcgccagcg tttcgtactg
    28021 gtttgccatc gcgttatctc cggcagcttt gctggcgcgg gcgaggattt tttccatttt
    28081 aaacatcagg ctgttcagat cgaccggtac gatgctggtg gtgcgtaagg tatttaactg
    28141 ctgcggattg tccatccagc gcgagctgaa atcccagcca gacgcagcgg cagagcgcag
    28201 gtcgcggtaa atttcagtgg caggtcgatt cggattgctt ttggcggtgg caatatcttc
    28261 cacccatgac tctggtcgtg gcgtatcgcg atcgtcccag tagcggttga gaagggtacc
    28321 atcctgaagt ttgacaacgc gtttttcctg ttgtccggct tgcaggtttt caacaccgtc
    28381 catccagtaa gcatattctt tttgcatttg cggcaggtat tgcttcaacg cggcatcgcc
    28441 ttcatgctgc gccagtaact ctaccatcag ggcaaagaat ggcggttgtg agcggcttaa
    28501 atagtaactg cggttgccgt tgggaatatg cccgtaagtg tctatttcat gagcaaaatt
    28561 ggccaccata tccgcgactt tatcccagtg accactttcg gcaagcccta acatggtgaa
    28621 gtaactgtcc cagtaatata cctcgcgaaa acgtccgccc ggcacaacat aaggttccgg
    28681 cagcggtaac agagaatccc atttttcggt attttcggta gaacgcgtta ataccggcca
    28741 aagtccgtca atatgttcgc gtagtgattg cccctctggc ggaacatatt tctcgccctc
    28801 tttcggcagg gtgaaattga cgttaacgaa atggcgtaga tcaaatccgc tctggttttg
    28861 ctgcatccga taatcagcaa ggatcatcag cggatcgctg ttcggcacgg catcggcaaa
    28921 ggttttttgg tccggaaaaa gtttggcgtt ttgcacatca ttaaacagcg gccctaataa
    28981 aatatcaggc ggctgtggcg ttaccggtgt ttcttctgcc tgcaccgata gcgcagcgaa
    29041 acacaaaaag atgcaggctg gaattaacgc catttttagc gggcgagaag ctgcggggga
    29101 tttcatcaat cattctcctt tggcgaaacc gaataaaacg gtattcaagt atcagaaaac
    29161 cttagttcag gatcgcacta gctgcatgat cgaaatctca ttctgcgaac ggtcaggcat
    29221 tttagaaaag ggaattaaag ggataaataa cgtcgaaata gagtgatcaa ataacgtctg
    29281 tttgttagcc agccagcggc gttgggttcg cgattagcgc agaaaaaatc atcatgattt
    29341 ggtaaaaaat ataacaggta gttaaaaatc atccgtgcag agtaaatagc cggatggcat
    29401 cagaacgatg ccatccgaac ggtggtttaa ccctgacggc tgaaacgttg cgttttaacg
    29461 tccagcgtta gcgtttcttc tggttgtatc gcatacagtt tctcaccctg ctggcaaatc
    29521 cagccaatcc ccagttcacg ggcgatcagg gcgctgtggg atagcggact tccggcgcta
    29581 aggcagatac cttttacaac cgccggatcc agttgcagta ttgtggaggg atagatgttc
    29641 tccgccagta gaatagttgg cgagttaaac tgcgggagtt cttctttcgt ttgggtcagg
    29701 tggaccaggg tgcgatgcag aagatcgtcc acatcaatat agcgagcctg tagatattca
    29761 tcatccagtt gctggtattg ctggctaagt tctttaagaa cttgctgcca ggcatattct
    29821 gccgtgcaat gttcatgctg aaggagttcg cttgccgccg ccagcagttc cggatcatct
    29881 aacagtgtat ggtgaccaga aaagattgcg gcaatatcgt caagcccgct ggcttctgct
    29941 ttcgctgtta acgtcatcag atctaataac gtgaagtcaa tggcctggcg taatcgttct
    30001 tgttcttcat ccacggtcag ggttgatttt gcctgtaccg tacataaaac cggttgataa
    30061 taaaaggctt tacccgaaac aggcggaacg ggacgcagaa taggtggagc aacttcctcc
    30121 gtttcaccaa agttatcttc agccagctga cggaaagcga tcagtgcctc ttcagcttct
    30181 ggccctttcg caatcaggcg cagcgtatcg ttatagcgaa cttgtagtaa cgcaatctgg
    30241 ttaatactct ccggtgtgac gcatttgccg tttttctcca gcaacatatc ggcattaaat
    30301 gtcgataagg tataaaccag ccgggaggcc ggacgtacat gcaggccgtt acggtttttt
    30361 atgaccaccg ccagagaacg ggcttcttca tcgtacgaag gacatgtgtc agagatttca
    30421 gtgttggagg agggtaaacc cagttgttca cgtttggctt ccagcgcatg catggcgtca
    30481 aagatcactt tgtcgatctc cgcccccgag gccgcgctaa ccgttgctgc cagtgtacct
    30541 tcgaccaacg gcgcagcaca caaacgtact tttgcggcga tctcgggagc cagcaattcc
    30601 agcgcagttt cagcactcaa taatgcgctt cctatatcca tcatgaccag cacatggtcg
    30661 gtatcggcaa cagattcgat ggcctccatc actttgacgg catcggtacc aatgggattt
    30721 tgtggatcgt caattcccgc ggcaatggcg attttacaat tatcactcat taacatctga
    30781 cgggctaatt caccgacgcc ttctcccagt cggctgctat gtgaaactat gaccaggttt
    30841 accatcacca attccttact cttttgcggc taacgccaac atttgcatca taaacatcac
    30901 cgaggtcgca ccggggtcct ggtgaccaat actgcgttca ccgagataac tggcacggcc
    30961 tttgcgagct tgcatcgtaa tcgtactttg tgcagcggat tcggcgacgc tactggcagc
    31021 ttcgagcgcc gccggaatag agagattttg ctcgctggac tgacgtaacg attccaccac
    31081 cggcacccac acatcacaca tggttttatc gccaggttcg gctttcccgc gactgattac
    31141 gccgtccgcg ccatcgcgga acatctgata aagctcttcc agcgtcaggc tttgccgtgc
    31201 ctgggtcgcc tgtgcggcgc ggataaagaa ggtaccgaaa agcggaccac tggcaccacc
    31261 gacgctggaa agtagcgtca taccggtatt cttgagaatg aaaccgatat ctttatctgc
    31321 gatagcaggg agtttttcca ccactttgct aaagcctcgg ttcatattta gcccgtggtc
    31381 agcgtcgcca atttcgcgat ccagtccggt aagatactcg ctctcggtgc tgaaaatatc
    31441 gccacaacga gtgagccagt taacaatttg agttctgctc agtgacattg ctatctcctt
    31501 atttacccca gttaagggcc ggggtgtgga ccggggcgtc ccagagtgcc agcgtttcgt
    31561 catcaacttt cagtaaggtg attgagaaac cggtcatatc cagtgaggtg cagtacgcgc
    31621 caattaaatt acgctcgata gtcaatcccg cttgctggca acgtgtggtc aggcggttat
    31681 agacgccgta cagctcagaa agcggagttg cgccaagatt gttaaccagc gcaatcaccc
    31741 gatcgccaga ctggagcggt tgtttagttt gtgattcttc ctgccaactg ccttgttgat
    31801 aatcccagaa acgcaaagtg cgatggtatg agccattttc cagcagggtg tcgaacattt
    31861 catcgacggt ttgatcaagg gaagagaagg gacggcggtc aatacctggc tcaccatgaa
    31921 tgccgacgcc aaactccatc tcattgtccg ccagggtaaa agaaggtttg cccgcggcag
    31981 gaacggtaca ggcaccgaga gcgataccta ttgagtggcc ttgattattc aacttacgcc
    32041 ccagttccgc acaggcgtcc agtgagtcgc cacgctcggc cgctgcgcct acgagttttt
    32101 caattaatac ggtgttggca acgccgcgtc gcccggcggt ataaagactg tcttttaccg
    32161 caacgtcgtc atcaatgacc acagtggtca cttttacgcc gctatcgtgc agtaactcgg
    32221 tcgctgtttc aaagttaaga atatcgccgg tgtaattttt gataatcaac agtacacctt
    32281 cgccgccatc aatttgcatg gcgcattcaa agattttatc gggcgtcggt gaggtgaaaa
    32341 tttcgcccgg acaggccccc gaaagcatcc cctgaccgat ataaccacaa tgcatcggct
    32401 cgtgtccgct gccgccaccc gacagcaggg cgactttacc tgcaacgggg gcatcagatc
    32461 gggtgacata caccggatcc tggtgcagtg tcagagatgg atgcgctttc gctagtcctg
    32521 ccagttgttc gtccagtacg tcttgcacat cattgatcaa ttttttcatt attttgctcc
    32581 agcaattacg gtagggcatg gatgatgttc aacgacacgg cgaccactga ctgccgatga
    32641 atccattgtg catcaggcaa gggaaaagaa aattagccca aattatgttt catagtgaaa
    32701 catattcttt aatgaatgtt ccatattgaa acttttacgt gtattaatac ttaaaattgc
    32761 gagccggaac accttttgtc ataagggatg cgggatatga gtagcgcttt taacaacgat
    32821 ggtcgggaca tatctccctt aattgcaacc tcctgggagc gatgcattaa gctgatgaaa
    32881 cgggatacat ggaacgtacc acaccaggcc cagggcgtga catttgcgtc tatttatcgg
    32941 cgtaagaaag cgatgctgac gctcgggcag gctgcgctgg aagatgcctg ggaatatatg
    33001 gcaccgcgag cgtgtgcgct gcttatcctc gatgaaaccg cctgcattct cagccgtaat
    33061 ggcgatccgc aaaccttgca gcaactaagt gcactgggat tcaatgacgg cacgtattgc
    33121 gccgagggaa ttattggtac ttgtgcgcta tcgttagcgg ctatctctgg tcaggccgtg
    33181 aaaacgatgg ccgatcaaca tttcaaacag gcactctgga actgggcctt ttgtgcaacg
    33241 ccgttgtttg acagcaaggg ccgattgacg ggaacaatag cgctggcgtg tccggttgag
    33301 caaactaccg cagctgattt gccgttgacg ttggcaatcg cacgcgaggt cggaaattta
    33361 ctgctgacgg acagtttgct cgctgaaact aaccgtcatt taaatcaact taatgccctg
    33421 ttagaaagta tggatgatgg cgtgattagc tgggacgagc agggtaattt gcaatttatt
    33481 aatgcccagg cggcgcgggt cttacgcctt gacgcgacgg caagtcaggg acgggcaatc
    33541 actgaactct taacgttacc cgccgtattg caacaagcaa taaaacaggc acatccgctc
    33601 aaacacgtag aagcaacctt tgaaagccag caccagttta ttgatgcggt gataaccctt
    33661 aaaccgataa tagaaacgca gggaaccagc tttattttgt tgctccatcc tgtggaacag
    33721 atgcggcagt taatgaccag tcaattagga aaagtcagcc atactttcgc tcatatgcca
    33781 caagaggatc cgcaaacccg ccgtttgatt cattttggtc gccaggcggc gcgcagtagc
    33841 tttcctgtcc tgctttgtgg agaagagggc gtgggcaagg cactgctaag tcaggcaatt
    33901 cataatgaaa gtgagcgtgc agcagggcct tatatcgccg tcaattgtga gttatatggt
    33961 gatgcggcgc tggcggaaga atttattggc ggcgatcgca cggacaatga aaatggtcgt
    34021 ttgagtcgac tggaactggc gcacggcggc acgctgtttc ttgaaaagat tgaatatctg
    34081 gcggtggagt tacagtctgc tttgcttcag gttatcaagc agggtgttat cacgcgactg
    34141 gatgcgcggc gtttaatacc cattgatgtc aaagtgattg ccacaacgac cgcggacctg
    34201 gcaatgctgg tggaacaaaa tcgttttagt cgccagctgt attacgcgct gcatgcattt
    34261 gaaattacca tcccgcctct gcgtatgcgg cgtggcagca ttccggcgct ggtgaataac
    34321 aaattacaca gtcttgaaaa acgcttctct acgcggctga aaattgatga cgatgccctc
    34381 gctcgcctgg tttcctgtgc atggcctggc aacgattttg aactttacag cgtcatcgag
    34441 aatcttgctc tgagtagtga taacgggcgc attcgcgtca gtgatttacc tgaacatctc
    34501 tttaccgagc aggcgacaga tgatgtcagc gccactcgcc tttccaccag tctgtcattt
    34561 gcggaagttg aaaaagaggc aattattaac gcagcccagg tcacaggcgg tcgcattcag
    34621 gaaatgtcgg ctttacttgg aatcggccgc actacgctgt ggcggaaaat gaagcaacat
    34681 ggcattgatg cagggcagtt taagcgccgt ggatagcggg agagtatgta ctttcccgct
    34741 atgaattgct aattaatagc gaccatgact gccgtgattt ggtccaccat ttgaaggtgg
    34801 cattggctta ccggagggcg caggaccacg agggccatcg ctgtggcggt gatgatggtg
    34861 gggaacacaa gcacttaaca ggctcgcaaa gacaattgct aacaggctga aggtaaatgt
    34921 ttttttcatg acaaactctt gaacggttaa tgcactgtga ttgttatcac ataaaaggca
    34981 cattgttaat attgccagga gtctgattat gattttcgat aactcagaat tgaaagaagc
    35041 cgcgtagcag gcg
//
LOCUS       DAZKJR010000037        34194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKJR010000037 DAZKJR010000000
VERSION     DAZKJR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.037"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHOWS_001841"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHOWS_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFQ0047417.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="ACHOWS_001842"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="ACHOWS_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFQ0047418.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHOWS_001843"
     CDS             complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHOWS_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFQ0047419.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHOWS_001844"
     CDS             complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHOWS_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFQ0047420.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHOWS_001845"
     CDS             complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHOWS_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFQ0047421.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHOWS_001846"
     CDS             complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHOWS_001846"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFQ0047422.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHOWS_001847"
     CDS             complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHOWS_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFQ0047423.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHOWS_001848"
     CDS             complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHOWS_001848"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFQ0047424.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSAMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICTYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHOWS_001849"
     CDS             complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHOWS_001849"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFQ0047425.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGSGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHOWS_001850"
     CDS             complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHOWS_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFQ0047426.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHOWS_001851"
     CDS             complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHOWS_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFQ0047427.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHOWS_001852"
     CDS             complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHOWS_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFQ0047428.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11268..12308
                     /gene="gpr"
                     /locus_tag="ACHOWS_001853"
     CDS             11268..12308
                     /gene="gpr"
                     /locus_tag="ACHOWS_001853"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFQ0047429.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHOWS_001854"
     CDS             complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHOWS_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFQ0047430.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13033..13917
                     /gene="yghA"
                     /locus_tag="ACHOWS_001855"
     CDS             13033..13917
                     /gene="yghA"
                     /locus_tag="ACHOWS_001855"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFQ0047431.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     ASYVTAEVHGVCGGEHLG"
     gene            complement(13956..14381)
                     /gene="exbD"
                     /locus_tag="ACHOWS_001856"
     CDS             complement(13956..14381)
                     /gene="exbD"
                     /locus_tag="ACHOWS_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFQ0047432.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTV
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14388..15122)
                     /gene="exbB"
                     /locus_tag="ACHOWS_001857"
     CDS             complement(14388..15122)
                     /gene="exbB"
                     /locus_tag="ACHOWS_001857"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFQ0047433.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15374..16561
                     /gene="metC"
                     /locus_tag="ACHOWS_001858"
     CDS             15374..16561
                     /gene="metC"
                     /locus_tag="ACHOWS_001858"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFQ0047434.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLLPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16701..17360
                     /gene="yghB"
                     /locus_tag="ACHOWS_001859"
     CDS             16701..17360
                     /gene="yghB"
                     /locus_tag="ACHOWS_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFQ0047435.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17400..18299)
                     /gene="yqhC"
                     /locus_tag="ACHOWS_001860"
     CDS             complement(17400..18299)
                     /gene="yqhC"
                     /locus_tag="ACHOWS_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFQ0047436.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18493..19656
                     /gene="yqhD"
                     /locus_tag="ACHOWS_001861"
     CDS             18493..19656
                     /gene="yqhD"
                     /locus_tag="ACHOWS_001861"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0047437.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19761..20588
                     /gene="dkgA"
                     /locus_tag="ACHOWS_001862"
     CDS             19761..20588
                     /gene="dkgA"
                     /locus_tag="ACHOWS_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFQ0047438.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20788..21714
                     /gene="yqhG"
                     /locus_tag="ACHOWS_001863"
     CDS             20788..21714
                     /gene="yqhG"
                     /locus_tag="ACHOWS_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFQ0047439.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTTNNPDEDM
                     RIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21765..22022
                     /gene="yqhH"
                     /locus_tag="ACHOWS_001864"
     CDS             21765..22022
                     /gene="yqhH"
                     /locus_tag="ACHOWS_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFQ0047440.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22064..24283)
                     /gene="ygiQ"
                     /locus_tag="ACHOWS_001865"
     CDS             complement(22064..24283)
                     /gene="ygiQ"
                     /locus_tag="ACHOWS_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFQ0047441.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(24394..25806)
                     /gene="ftsP"
                     /locus_tag="ACHOWS_001866"
     CDS             complement(24394..25806)
                     /gene="ftsP"
                     /locus_tag="ACHOWS_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFQ0047442.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPTLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25881..26618)
                     /gene="plsC"
                     /locus_tag="ACHOWS_001867"
                     /gene_synonym="parF"
     CDS             complement(25881..26618)
                     /gene="plsC"
                     /locus_tag="ACHOWS_001867"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFQ0047443.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26852..29110)
                     /gene="parC"
                     /locus_tag="ACHOWS_001868"
     CDS             complement(26852..29110)
                     /gene="parC"
                     /locus_tag="ACHOWS_001868"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0047444.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29248..30855)
                     /gene="ygiS"
                     /locus_tag="ACHOWS_001869"
     CDS             complement(29248..30855)
                     /gene="ygiS"
                     /locus_tag="ACHOWS_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0047445.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGIKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(30964..31446)
                     /gene="ygiV"
                     /locus_tag="ACHOWS_001870"
     CDS             complement(30964..31446)
                     /gene="ygiV"
                     /locus_tag="ACHOWS_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0047446.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(31499..31891)
                     /gene="ygiW"
                     /locus_tag="ACHOWS_001871"
     CDS             complement(31499..31891)
                     /gene="ygiW"
                     /locus_tag="ACHOWS_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFQ0047447.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32043..32702
                     /gene="qseB"
                     /locus_tag="ACHOWS_001872"
     CDS             32043..32702
                     /gene="qseB"
                     /locus_tag="ACHOWS_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFQ0047448.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32699..34048
                     /gene="qseC"
                     /locus_tag="ACHOWS_001873"
     CDS             32699..34048
                     /gene="qseC"
                     /locus_tag="ACHOWS_001873"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFQ0047449.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(34094..>34194)
                     /locus_tag="ACHOWS_001874"
     CDS             complement(34094..>34194)
                     /locus_tag="ACHOWS_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HFQ0047450.1"
                     /translation="REMFEFWLYVISLVICLVCGWWLFWGRFIFCY"
BASE COUNT         8350 a         9171 c         8661 g         8012 t
ORIGIN      
        1 ataatcagta cggggttgct acaacatgta ttactctttc accacaatta gcggttcaat
       61 atcactctct tttttgatga ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttaccg ctggttttgt ccagcacctc
      241 ttcatgatgg ctgacgaggt cgatgttact gccacagcga ccggcgatcg gtttcactgc
      301 ataaccggtt ttcaccagtt catcattcac ggagaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcactgtcca
      421 cagaggttca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcatt
      481 ttgcggatga ccggtacgga tcggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccagcccgc gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccagttcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agccagcttc ggtatgacag gaggcggagt cggcgttgta
      901 ttcgtagacc ttcaggccac gttcgtccat acagaaatcc atacgaccgg tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggatttttg ggatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagttcgttg gtggctttaa tcagctcctg ctctgcgctc tcggtaatgg tgtagtaatg
     1141 atacggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gtagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccaggcgcg ctccgctgat
     1261 tttcaacagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg
     1321 gatcatccag ccgagaatgg tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt
     1381 ttcgaccacc atctccagct cgcgcgtcca ctgttgccct tgcggtaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgcaat tgggtaatga tggcgacatg
     1501 accagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttcacg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtgaaga ccacaccgta
     1681 attcagaaag agaaaacggc gagcaaattc aacgcattgc catttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaacactt atcagcccgc gaaagtctgg acgtgggaca aatccgccgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa
     2221 cacccattgc aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tggcgctggg cgttactggt gcagagtacg acgcctggct gattcgtatt
     2341 ggcgatggcg atcaattctc cagcggcttt gtcgaagtga acccaaactc gaagatcccg
     2401 gcgctgcgcg atcatacgca taatccgccg atccgcgtgt ttgagtctgg atcgattctg
     2461 ctttatctgg cggagaaatt tggctacttc ctgccgcagg atctggcaaa gcgtactgaa
     2521 acgatgaact ggctgttctg gctacagggg gctgcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc ggtgaaaatt gagtacgcca tcaatcgctt taccatggaa
     2641 gccaagcgcc tgctcgacgt gctggataag caactggcgc agcataagtt tgttgcaggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaacgt ggtgttaggc
     2761 ggcgtgtatg atgccgcaga gtttctggat gcgggaagtt ataaacacgt acaacgctgg
     2821 gcgaaagaag tgggcgaacg tccggcagtg aagcgtgggc gtattgttaa ccgcatcaac
     2881 ggaccgctga acgagcagtt gcatgaacgc catgacgcca gtgatttcga gacgaatacg
     2941 gaagataagc gtcaggggta agggttggta ttcgtcgcag caagccatcc aggccggata
     3001 aggcgttcac gccgcatccg gcaatcgagc acaatgcctg atgcgacgct gccgcgtctt
     3061 atcaggccta caaaagctaa tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagc
     3121 gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt
     3181 accagtaccc actggcccag cagatcggca gggttacctt cacaaatcag ggcgatattc
     3241 acgtcgcgct tgataccaca tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca
     3301 gccagcacct ggcctggaac gccaatacac attgttaact ccggttattc aacttcaata
     3361 cttttgacga ttagcgaatc gccggtatcg acacgcaacc gctcgccatg acagatcgga
     3421 cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca ccaggcctgg
     3481 gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
     3541 tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcaccaat ttccagccac
     3601 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gttggataat ttcaacggcg
     3661 ctctggcaaa gcgacaactc atgcattttc gccactcctg cggccaaata gcagggcacg
     3721 acgccctgcg tgtggtacat ccggattggt gactggcagc gaaaggagca ttcgggcgca
     3781 gtcatccgtc agccgttggc cctcttcaat cgacatgctg tgcgaaagcg gcgacatcag
     3841 cgagcaggag aggtattgcg aaacaccatc cagttcacca acagtaaagg tcatagtgcc
     3901 atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg
     3961 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
     4021 ttcgaacagc gtaaagtcag aaacatacac cggcattgaa ggatgcagaa aagagagatc
     4081 atgcatcgaa cgccgggcaa tttcttcaaa agctgcctgt acttgcgcct tcggggaggt
     4141 ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgcctg
     4201 attcacgcag cgcagccaga acctgctcaa gcgcaggttc aatcatcgct tcgaccgtcg
     4261 gcgtcaagcc aatgtgcggc tccagtgatt ccgggatcac gccgaccagg gtcagctttt
     4321 tcggaaactc accggtgaag cgcagggccg acaatacgtc ggccaggcca agctgatgcg
     4381 gagagatttt gttggtaaac aacgccggaa cttcttcatc tcgcaggatc atcatcgttc
     4441 ccggcgcgtt ctttttcgag acaatggcat ccgcaataat cagatgatcg cgatttgcca
     4501 tgtcgccaag cagctccatt cccgccgttc cgccatcgag gatctcaaca taatccggca
     4561 gaatgtatcg ttgctctaac gcttcaacaa ttcgcacacc gatggcttca tcggtcagca
     4621 aaatattgcc aacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
     4681 gttgccgtcg gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat
     4741 ggtacgcacc acttccagcg gtttattcgg atcggcaacc ggtgtaccca ccagcgactg
     4801 ctcgtaagga ccgacgtcat cattgaagtt acgcgggcca gagttccagg ttgatggaac
     4861 aactgcctgg tagttactga taataccgtc tttgatcacc atccagtgag agagcatacc
     4921 gcgcggtgct tcaaggaaac caacgccttt gaactcacct gtagccggaa tgttcggctt
     4981 cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attgattttg
     5041 caggatatcc tgcaattcgc agcagtgaac ggtacgacca ataatacggc ccagcgtgga
     5101 gtgcagctgc gccacttcca gcgtgttgcc agtcagtttc tgataaatcg caacgatttc
     5161 attcagtttg ttctgggtag actcgcgtcc tgccgccagt ttcaccagca tgttagccag
     5221 cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttacc
     5281 gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggtg cctgcggcgc
     5341 ttcgtcttta taccaggagt gcttcgcgct ctcctgaatg cctttaatca ggtattcatc
     5401 ggagtgggaa gtgatcgggc gatacgagga cagatccgca ttctcaatgt agccgcccgg
     5461 gaacaggaag ctgccgtttt tgctgtcggt cgggaattcc ggcacgctca ggtagttcac
     5521 cgcaccttta ccgcgcgtca gccattccgg gtagaacgcg gcgataactg cggtatcaac
     5581 cttgtaaacc tgctcaacaa agtcgctcag tttatcgatg aaagacttga tgtacatcag
     5641 gcgctcaagg ttcagcacgc ccagaccgtc gaggttgatc gggttagcga ccccacctac
     5701 tgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc
     5761 acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg
     5821 cagtttcatc gccgggtgac cccagtagcc attggcgaaa atccccaact gaccgctggc
     5881 aaccagatct ttaatcttgt tctgaacttt ggtgaactct tccggactgt tcagatgcca
     5941 ggtcgaaacg cctttcagca tttcagaggc tttggttggg tcagcttgca gtgcagaagt
     6001 gatgtccacc cagtccagcg ccgagagctg atagaaatga acgatatggt catgcgtggt
     6061 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
     6121 tgcactttct gccgcacgaa cggaagagag cgcgtgagta gtcgtacata cgccacagat
     6181 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
     6241 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
     6301 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
     6361 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
     6421 cgaatgagta cgatgtaagt gcaaatctca atagccacaa aaccaataga aatcaacagt
     6481 tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag
     6541 gtcagacgcc aggttgcaca acctaacagc gcgctcagtg ctgacaggaa cagcatgcgg
     6601 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
     6661 tcaatccaga acatcgcgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
     6721 tagataagct cgccaaagcg cagcacgatg aaaatcgcca gcaacacact gatggtgttg
     6781 gtcagcttga cgaacaggct cttttcatct ggaccattgc cacgcagacc cgcctgcacc
     6841 aacgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
     6901 agcggcaaca tttcatagct ctgccacaac ggatgcactt tgtagcccgc cgagatcatc
     6961 agcgacccca ttgaagactg gtgcatagtt ggcagcagcg caccgagcgc gataatgaag
     7021 aacatcacct tgttcagacg ctgtagcgac accttccagc ccaatcgttc aaacaatgcc
     7081 ggagcaaact ccagtgccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
     7141 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
     7201 ccaacgtcga tggtgatcga caagccaccc agtgagtaac cgaacagact cgccaacagc
     7261 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgccagc
     7321 gcccagccgc cacaggcaaa gccggtgccg atcaacaggt caaacgcgat ccacacgccc
     7381 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
     7441 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
     7501 ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
     7561 tttggtgttg cgacgaacca gcacggttaa gcccgccagc acagccagtg gtagcatcat
     7621 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
     7681 tttcggcagg tcgagatttt cgtaaggcac accggtcagt accagaacct gagtaccgcc
     7741 gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc
     7801 gccagatttc agcgtctgac gcggatagtg gtattcgctg cctggcttca gcgccagacg
     7861 ttttttcgcc tcagccatca gctcttcacg cgtaccaaaa atcaccgcgc ccgccgggca
     7921 cacttctacg cagccaggca gaccgccttt atcaagacgt tccacacctt tctggttgca
     7981 cagttcacac ttatgcagcg caccaaatgg gttgttgtag tcgtacttcg gcacgttgta
     8041 tgggcaggcg accatgcagt aacggcagcc agtgcacaca tctttgtcgt aatggacaat
     8101 gccggttttc ggatcttttt tcagcgcaga gaccgggcac acagagacac aattcggatc
     8161 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
     8221 gacccccgag ccgctggtcc acacctgaat gatgttattg gtgtacggcg acagtttgtc
     8281 gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaaattga tatcctgaca
     8341 cttggtgacg caagcctggc agccaacgca caaggtcgag tcgtacaaca tccccagcga
     8401 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt
     8461 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
     8521 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
     8581 acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcccgc agagacgttg
     8641 ccgccttctt tagcgttaac gtccggtttc tgtgaacgcg gagtctgatt ttcgacgttg
     8701 gcaagctgat ggatgccttt atggaagccg ataccttctt cgttacagcc atagcacggg
     8761 tgaccaatcg ctaccggcca cacgccgcca acgtcgcaga attgcagcgt tgagcagttg
     8821 ccgtatgttt ctggcccttt acagccgagg tggtacaggc accagccttc gcggtggcct
     8881 tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt
     8941 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggc
     9001 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
     9061 cccggaatat tgataacggt tttgcctggc agaacttctt gcaggctgac tgcgccagtt
     9121 gggttaactc cagcggcggc aacaccgccc cacgcagagc aggaaccgat agcgataatg
     9181 gctgctgcgc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
     9241 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
     9301 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct ggtgaccgaa agcagcagaa
     9361 agcacctcgt gatactccag agagatagtc tccagcacga ggttttctac cgttggatgc
     9421 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
     9481 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
     9541 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
     9601 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgaa gacctggcat
     9661 atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta
     9721 ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta
     9781 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg
     9841 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
     9901 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
     9961 ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    10021 cacagtattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    10081 agcctttacc aaaatggtta ttcatcgttc acctcattcg ctatatattg ccgtatatag
    10141 cgatatttta ggcaaagaac gacatttgcg cacgctattt tttcatgctt gtttaagacg
    10201 taatcctgta agagataaac ccatcaggag agatatttat cgaaccattt cagcgtcctt
    10261 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    10321 tgattaaccc ccggatatat atacgcctca tagaccttat tattggcttt caacgccgcc
    10381 tcgtaagcag gccagccctc gttgattcgg gtgtccagtt ccgcgtagtg caacagtaaa
    10441 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcaccgcg
    10501 caggccagtt ccgggtaagc gaccgctgcc gcgttcgata cgccaccgcc atagcaaaat
    10561 ccagtaatac ccactttgcc tgtcgcttgc ggatagcttt gcataaactc aatcgcggca
    10621 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    10681 tcattacccg gataacctcc aacggaactt aagccgtcag gtgccagggc gatatacccc
    10741 gccttcgcca ctcgccgcgt cacatcttcg atatacggat tcagccctcg attctcatgc
    10801 accactacca cggctggcgt tttgccgctc atttttgcgg gcttcaccag ataaccccgt
    10861 acctcgccgt gaccatttgg cgagggatac gtgatgtact ctgcaacaat ttccgggtcg
    10921 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa cagcgctaat
    10981 gccgtcatcc cgcccaccgc atacttcgcc gcaaggttga ggaactcacg tttcgagatt
    11041 ttcccgtgag cgtaatagtc gtagtaatcc agcaactctt gtgggaaatc tttggcggtt
    11101 aaacgcggca tcgttgcact cctcaactgg tgttttttta agcaaagcat aagcgcgtgt
    11161 ttttgcccag tttttcgtca ctctgtgagc cagactaagg gatacgcgct ggcgaatcgc
    11221 taaactagaa acattgtttc gaaattgaac gatggaaagg agaggtcatg gtctggttag
    11281 cgaatcccga acgttacggg cagatgcagt accgctattg cggaaaaagt ggtttacgcc
    11341 tgcccgcgtt atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac
    11401 agcgtgcgat cctgcgtaaa gcgtttgatt taggcattac gcactttgat ttagccaaca
    11461 attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg cgcgaggatt
    11521 ttgccgctta tcgcgatgaa ctgattatct ctaccaaagc cggatacgat atgtggcccg
    11581 gcccgtacgg ctctggcggc tcacgtaaat acctgctcgc cagcctcgac caaagcctga
    11641 agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc
    11701 cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg
    11761 tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat ggttgagttg ctgcacgagt
    11821 ggaaaattcc gctgttaatt catcaacctt cgtacaattt actgaaccgc tgggtggata
    11881 aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgcattgcc tttactccac
    11941 tggctcaggg attgctgacc ggaaaatatc tcaacgctat tccgcaagat tcacggatgc
    12001 atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gctcaccgaa gccaacctca
    12061 acagcctgcg cttattgcat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg
    12121 cgttaagctg gttgctgaaa gatgagcgag tgacgtcggt attgattgga gccagccgcg
    12181 cggagcaact tgaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc
    12241 tggcgcagat tgatcagcat atcgccgatg gagagctgaa tctgtggcag gcgtcttccg
    12301 ataaatgacc tgttaataac gggcgcgaga accgcgcccg aatcagatca gtgattatga
    12361 cgagtcagtc ggtcaagata gcccatcaca aatgcagaga gcacaaacgt cagatggata
    12421 atgacgtacc acatcagttt gttatcaggg acatttttcg catccataaa gacgcgcagt
    12481 aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgcg
    12541 tccattttcc ccagccagtt cagcttctct ttgttctcag agatatcgag ttgtgagacg
    12601 aaattctcgt aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc
    12661 accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt
    12721 acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca
    12781 aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc
    12841 atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattaag taaacagcac
    12901 gcaacagcaa atcaacatat acgttaaacc tttgtcgcat aattctgagg gttagggatc
    12961 ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa
    13021 gggagaaaaa tgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat
    13081 cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccggt accggattgc
    13141 ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg
    13201 ggcgattccg gaatcggtcg cgctgccgcc attgcttacg cgcgtgaagg ggctgacgtg
    13261 gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtcaaaaa gatcattgaa
    13321 gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt
    13381 tcgctggttc acgaagcaca caaggcgtta ggcgggctgg atattatggc gctggtcgcc
    13441 gggaaacagg ttgccattac tgatattgca gacctcacca gcgaacagtt tcaaaagacc
    13501 tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg
    13561 aaaggtgcca gtatcatcac cacttcgtca atccaggcat accagccaag cccgcattta
    13621 ctggactatg cggctacgaa ggcggcaatt ctgaactaca gccgtggcct ggcaaaacag
    13681 gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gcccgatctg gacagcactg
    13741 caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagaa aacgccgatg
    13801 aagcgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag
    13861 gcgagctacg tcaccgcaga agtgcatggc gtgtgcggcg gcgagcattt aggctaaaaa
    13921 aatgcccggt tgtgaaaagc aaccgggcat catttttatt tcgctttggc agtttcttcg
    13981 ccgaccagac ctatcttcag gtaacccgcc tgatgcagcg tatccattac cttcatcaac
    14041 gtctcgtaat cgacggtttt atccgctcgg aagaagacgg tggtgtcttt cttgccttcg
    14101 gttaacgcat tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt accgataaac
    14161 atcgagttgt ctgccttcac cgacagataa accggttttt ccgggcgcgg ctgcggcgtg
    14221 ctggtagaag caggcaagtt caccttcaca tctaccgtcg ctaacggtgc cgccaccata
    14281 aagataatca gcagaaccaa catcacgtcg ataaacggcg tcacgttgat atcatgcatt
    14341 tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta tcctgcgcgt
    14401 aatttttgtg cgacacgaac cggatgcgca gcggcgctgg cttccagatc caggtcacgg
    14461 ctttgcagca acaatacctg cgctgcaaca tcacccagca tcgctttaaa gccgccaatc
    14521 tggcgtgcaa atacgttata gataacgacc gccggaatcg ctgccacgag gccgattgcc
    14581 gttgctaaca gagcttctgc gatacccggc gcaacgactg ccaggttagt agtctgcgtt
    14641 tgcgcgatac caataaagct gttcatgatg ccccagaccg taccaaacag accaacgaac
    14701 ggcgaaatcg cgccgatggt tgccagatag ccgttaccac ggcccatttg acgacctact
    14761 gcggcgaccc gacgctccag acggaagctg gtacgttctt taataccttc gttatcgtcg
    14821 ctgccttctg acagttccag ctcattctgc gcttcattga gcaaatgcag gctcaagctt
    14881 ttgctaccaa aatcagcggc gatatcgttg gcctggttta aggaacgcgc ttcagccagc
    14941 agttgctgct cgcgcttaag acgacgcttc tgattgaaga actccacgct cttactgaag
    15001 aagattgccc aggtgactac ggaggccaaa ataagcccaa tcatcacgca cttaacgaca
    15061 atatcggcgt gctgatacat accccagacg gagaggtccg tctgcattaa attattaccc
    15121 actgtgtatc tccaggacgc aagtcacaaa atctgcgcat aataatatca aaacgacgtc
    15181 gaattgatag tcgttctcat tactatttgc atactgccgt acctttgctt tcttttcctt
    15241 gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg aaaattttct gctttatgcc
    15301 aattcttcag gatgcgcccg cgaatattca tgctagttta gacatccaga cgtataaaaa
    15361 caggaatccc gacatggcgg acaaaaagct tgatactcaa ctggtgaatg caggacgcag
    15421 caaaaaatac acgctcggtg cggtaaatag cgtgattcag cgcgcgtctt cgctggtctt
    15481 tgacagtgtg gaagccaaaa aacacgcgac acgtaatcgc gccaatggcg agttgttcta
    15541 tggacggcgt ggaacgttaa cccatttctc cttacaacaa gcgatgtgtg aactggaagg
    15601 tggcgcaggc tgtgtgctat ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc
    15661 ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc gcctatgaac cgagtcagga
    15721 tttctgtagc aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg atccgctgat
    15781 tggtgccgat atcgttaagc atctgctgcc aaacactaaa atcgtgtttc tggaatcgcc
    15841 aggttccatc accatggaag tccacgatgt tccggcgatt gttgccgccg tacgcagtgt
    15901 ggtcccggat gccatcatta tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc
    15961 gctggatttt ggcatcgatg tttctattca ggccgccacc aagtatctgg ttgggcattc
    16021 agatgcgatg attggcactg cggtgtgcaa tgcccgttgc tgggagcagc tgcgagagaa
    16081 tgcctatctg atgggccaga tggtcgatgc cgataccgcc tatataacca gccgtggcct
    16141 gcgcacatta ggtgtgcgtt tgcgccaaca tcatgaaagc agtctgaaag tggctgaatg
    16201 gctggcagaa catccccaag tagcgcgagt taaccaccct gccctgcctg gcagtaaagg
    16261 ccacgaattc tggaaacgag actttacagg cagcagcggg ctattttcct ttgtgcttaa
    16321 gaaaaaactc aatgatgaag agctggcgaa ctatctggat aacttcagtt tattcagcat
    16381 ggcctactcg tggggcgggt atgaatcgtt gatcctggca aatcaaccag aacatatcgc
    16441 cgctattcgc ccacaaggcg agatcgattt tagcggaacc ttgattcgcc tgcatattgg
    16501 tctggaagat gtcgacgatc tgattgccga tctggacgcc ggttttgcgc gaattgtata
    16561 acattgccac ttttggacaa ttttgcagac attttattgt gaaaagtctt aaattgttgc
    16621 gtccgggatc aaggcgtccc ggacgattca ggagtacaat aggcagataa aggcttaaac
    16681 gctgttccac aggaaagtcc atggctgtta ttcaagatat catcgctgcg ctctggcaac
    16741 acgactttgc cgcgctggcg gaccctcata ttgttagcgt tgtttacttt gttatgtttg
    16801 ccacgctgtt tttagaaaac ggcttgctgc ccgcctcatt tttgccaggc gacagcttgt
    16861 tgatactggc aggcgcattg attgcccagg gggttatgga ttttctgcct acgattgcga
    16921 ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta tattcagggg cgctggttag
    16981 ggaataccaa aacggtgaaa ggctggctgg cacagcttcc tgctaaatat caccagcgcg
    17041 ccacctgcat gtttgaccgc cacggtctgc tggcgctgct ggctggacgt tttcttgcat
    17101 ttgtccgtac gctgctgcca accatggcgg gaatttccgg tctgccaaac cgccgcttcc
    17161 agtttttcaa ctggttaagc ggattgctgt gggtcagcgt ggtaaccagt ttcggctatg
    17221 ccttaagtat gattccgttc gttaaacgcc atgaagatca ggtaatgacg ttcctgatga
    17281 tcctgccaat tgccttgtta accgctggct tgttgggcac gctgtttgtg gtgattaaaa
    17341 aaaaatactg taacgcctga cgattttccc cattcccggt agctgtaccg ggaacgtatt
    17401 taattcccct gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac accgaagtaa
    17461 cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat cgccgccgcg
    17521 ctggccttca tgccgtcgtg gatgatcatc atccgcgcct tatgcagacg gtaattcttc
    17581 aaatactgca acggcgaggt gctggtgaca gacttaaaat tatggtggaa cgccgatacg
    17641 ctcatgttgg cttctgccgc cagttgctcg acgctcaggt tttcggtgta tttattctca
    17701 atccgtttca gcacgcgact aatcaggctg aagtgagtct ggcggctgac cagtgccagt
    17761 aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat gatctgtttg
    17821 ccgagaatac gcgcatccag cggtcgctcc atcacatcca gtaaccgctc cgccgcgcat
    17881 aaaatctctt ctgataacgt ggcagagtta atcccgctgg ctgccatcga cggctggaaa
    17941 tgctcatctt cgccaatgtc catcaacagt tcctgtaact gcaaaatatc gacattgaga
    18001 cgcaaccctg ccagcggcac ctctgacgtg gcataggttt cgcactcaaa cggcaacggc
    18061 accgtcagca ggagatattc attagcgtca taacgaaaca cgcgttcatt gatataaccg
    18121 attttatgtc cggaaaagag aattatgatg ccaggctcgt acatcaccgg tgtgcgtgcg
    18181 aaaggcgtct cgccatacaa caaacgcaca tcgggcaaca gctctgacaa actattttct
    18241 ttatttttca gtttattaac tttatccgcc agcaagcggc aaatctcttc acgtttcata
    18301 tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg cgcaattttg tagcatttct
    18361 ccagcactct ggagaaatag gcaagacatt ggcagaaatg agcattgaga ggtacggcgc
    18421 tggcgaccac aatgaaaaac atcaggcaga ccgttctctg ccctcatatt gacccagcaa
    18481 agggagcaag taatgaacaa ctttaatctg cacaccccaa cccgcattct ttttggtaaa
    18541 ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt gattacctac
    18601 ggcggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc
    18661 atggacgtgc tggaatttgg cggtattgag ccaaacccgg cttatgaaac gctgatgaac
    18721 gccgtgaaac tggttcgcga acaaaaagtg actttcctgc tggcggttgg cggcggttct
    18781 gtactggacg gcaccaaatt tatcgccgca gcggctaact atccggaaaa tatcgatccg
    18841 tggcacattc tgcaaacggg cggtaaagag attaaaagcg ccatcccgat gggctgtgtg
    18901 ctgacgctgc cagcaaccgg ttcagaatcc aacgccggcg cggtgatctc ccgtaaaacc
    18961 acaggcgaca agcaggcgtt ccattctgcc catgttcagc cggtatttgc cgtgctcgat
    19021 ccggtttata cctacaccct gccgccgcgt caggtggcta acggtgtagt ggacgctttt
    19081 gtacacaccg tggaacagta tgttactaaa ccggttgatg ccaaaattca ggaccgtttc
    19141 gcagaaggca ttttgttgac gctgatcgaa gatggtccga aagccctgaa agagccagaa
    19201 aactacgatg tgcgcgccaa cgttatgtgg gcggcgactc aggcgctgaa cggtttgatc
    19261 ggcgctggcg taccgcagga ctgggcaacg catatgctgg gccacgaact gactgcgatg
    19321 cacggtctgg atcacgcgca aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa
    19381 cgcgatacca agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa catcactgaa
    19441 ggttcagacg atgagcgtat tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa
    19501 ttaggcgtgc cgacccacct ctccgactac ggtctggacg gcagctccat cccggctttg
    19561 ctgaaaaaac tggaagagca cggcatgacc caactgggcg aaaatcatga cattacgctg
    19621 gatgtcagcc gccgtatata cgaagccgcc cgctaagctt tttacgcctc aaactttcgt
    19681 tttcgggcat ttcgtccaga cttaagttca caacacctca ccggagcctg ctccggtgag
    19741 ttcatataaa ggaggaacgt atggctaatc caaccgttat taagctacag gatggcaatg
    19801 tcatgcccca gctgggactg ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca
    19861 ttcaaaaagc gttagaagtg ggttatcgct cgattgatac cgccgcggcc tacaagaacg
    19921 aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca
    19981 ccactaagct gtggaacgac gaccacaagc gcccccgcga agccctgctc gacagcctga
    20041 aaaaactcca gcttgattat atcgacctct acttaatgca ctggcccgtt cccgctatcg
    20101 accattatgt cgaagcatgg aagggcatga tcgaattgca aaaagaggga ttaatcaaaa
    20161 gcattggcgt gtgcaacttc cagatccatc acctgcaacg cctgattgat gaaactggcg
    20221 tgacgcctgt tattaatcag atcgaacttc acccgctgat gcaacaacgc cagctacatg
    20281 cctggaacgc gacacacaaa atccagaccg aatcctggag tccattagcg caaggtggga
    20341 aaggtgtttt cgatcagaaa gtcattcgcg atctggcaga taaatacggc aaaaccccgg
    20401 cgcagattgt tatccgctgg catctggata gcggcctggt ggtgatccca aaatcggtca
    20461 caccttcacg cattgtcgaa aactttgatg tctgggattt ccgtctcgac aaagacgaac
    20521 ttggcgaaat tgcaaaactc gatcagggca agcgtctcgg ccccgatcct gaccagttcg
    20581 gcggctaacg tgcaaattct cccggtggcg gtaatgttcc gctaccggac ttttcagaaa
    20641 tcatttattc ccctcgcgtc ctgcccattg ttactcttcc ttgttcagga atgccaaata
    20701 taaggacatc atcatgcaga gccggaagct cttaaaggaa caactcatct acacccggga
    20761 taaacgcaac ggagaggtga aaaacagatg aaaataatac ttctgttttt agcagccctg
    20821 gcaagtttta ccgtacacgc gcagcctcct tcacagaccg tagaacaaac agtccggcag
    20881 atttatcaga actataaatc agatgccagt acgccttatt ttggtgaaac cggagagcga
    20941 gcgataactt ctgcgcgtat tcaacaggcg cttaccctga acgacaatct tacgctgccg
    21001 ggcaatattg gctggctgga ttatgatccg gtttgtgatt gtcaggattt tggcgatctg
    21061 gtgttagaaa gcgtagcgat aacccaacct gacgccgatc atgccgatgc cgttgtgcgc
    21121 tttcgtatct ttaaagacga taaagaaaag accacgcaga cactgaaaat ggtggcggaa
    21181 aatggtcgtt gggtcattga cgatattgtc agcaatcatg gcagcgtctt acaagcagtt
    21241 aatagcgaga atgaaaaaac gctggccgct atagcttcgt tgcaaaaaga acagccggaa
    21301 gcctttgttg ccgaactctt tgaacatatt gctgattata gctggccgtg gacgtgggtg
    21361 gtttccgact cttaccgcca ggcggtcaat gccttctata aaaccacctt caagactacc
    21421 aacaatcccg atgaagatat gcgaatagaa cggcaattta tttacgacaa tccgatctgt
    21481 tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc
    21541 gattccgccc gcattcatgt tcgttttacg ctgaccaatg gcaacaatga agagcaagaa
    21601 ctggttttac agcggcgcga aggtaagtgg gaaatcgctg attttatccg cccgaatagc
    21661 ggtagcctac ttaagcagat tgaggcaaaa accgccgcca gattaaagca atgagctgag
    21721 ttaaataaca attagccgga acaataaata aaagggaaca ctatatgaaa acgattttca
    21781 ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg
    21841 tcaatcagct ggcgagcaat gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg
    21901 atatgaaagc attacgccca caaatctatg ctgccaaatc cgacgctaac agagccaata
    21961 cgcgtcttga tgctcaggac tattttgatt gcctgcgctg cttgcgtatg tacgcagaat
    22021 gataaaaaaa ttcccggcag cattctgtta ccggggatat tttttaacgt ccaaccgccg
    22081 ctttagggcg tttcttcgca ccagcattct gcaggcgaga ttgcgtagac gacgcttttt
    22141 ttgccgtagc aggcgtctga cgctgggtcg ccatcggcgt atgtttagtc aacgccggac
    22201 gggtattgcg gttctggcga cgagcctcac gcatctcttc aatggttggc gcaggcacta
    22261 agcaatcgcg acggctgcca atcagatgct ttttgcccat cgcttccagc gcctggcgga
    22321 ttaacggcca gtttgccgga tcgtggtaac gcaacaacgc tttatgcagg cgacgctgct
    22381 tgtcgccctt cggtacaaag acgtcttcac ttttataacc aatcttcgcc agcgggtttt
    22441 tgccggtgta atacatagtg gtcgagttag ccagcggcga cgggtagaag ttctgtacct
    22501 ggtcgaggcg gaagcggtgc tttttcagcc acagcgccag attcaccata tcttcatcgc
    22561 gcgtacccgg gtgcgcggag atgaaatacg ggatcagata ctgttcttta cctgcctgtt
    22621 tcgagtaagt atcgaacagc tctttaaagc ggtcatagct gcccatgccc ggcttcatca
    22681 tcttcgataa cggcccttct tcggtatgtt ccggggcaat cttcagataa ccgccaacgt
    22741 gatgggtcgc cagctctttg atatagcgcg gatcttctac ggcgatgtca taacgcacac
    22801 cagaggcaat caggatcttt ttaatgcctt tcagatcgcg cgcacggcga tagaggttaa
    22861 tcgtcggttc gtggttagtg tccatatgcg gacaaatatc cggataaacg cacgacaaac
    22921 gacggcaggt ttgctcagcg cgtggcgatt tgcaacgcaa catatacatg ttggcagtag
    22981 gcccaccgag atcggaaatc acacccgtaa aacctggaac agtgtcgcgg atcgcttcga
    23041 tctcattaat aatcgaatct tcggaacggc tctgaataat gcgcccttcg tgctcggtaa
    23101 tagaacagaa agagcagccg ccaaagcagc cacgcataat gttgaccgag aaacgaatca
    23161 tttcgtaagc cggaattcgg gcattaccgt aagccggatg cggcacgcgc ttgtacggca
    23221 gcgcaaagac gctatccatc tcttcggtag aaagcggaat agcaggcggg ttgatccata
    23281 catagcggtc gccatgtttt tgcatcaatg cgcgggcgca gcctgggtta gtttcgtggt
    23341 gaagaatgcg cgaagcatgg gcgtacagca ctttatcgcc cttcactttc tcgaaagaag
    23401 gcagcaacac gtaggttttt tcccacggtt tcgggcgcgg cggctgcacg gttacggctt
    23461 tcgcttcctg ctttttcggc gcaacaggtt tgttatccgc gcacggcaga tcttcgccat
    23521 acggatgcgg gatcgggtcg attttcccag gagtatcaag acgggtggaa tccacgccgc
    23581 tccagcctgg caacgcctct tttacgataa tcgcggtatt acgcacatcg cggatttcac
    23641 taatcggctc gcccatcgcc agacgatgcg ccacttccac cagcggacgc tcaccgttac
    23701 cgaacatcag catgtcggct ttcgaatcca ccagcacgga acggcgcacg gtatcggacc
    23761 agtaatcata atgcgcggta cggcgcagac ttgcctcaat accgccgagg atcaccggta
    23821 catctttcca cgcctcttta caacgctggg tataaaccag tgtggcgcga tccggtcgct
    23881 tacccgcgac gttatccggc gtgtaggcat cgtcatgacg taaacggcga tcggcggtat
    23941 agcggttgat catcgagtcc atgttgccag cagtaacacc gaaaaacaga ttcggtttac
    24001 ccagacgcat aaagtcgtct ttgctgctcc agtccggttg ggcaatgatc ccgacacgaa
    24061 agccctgcgc ttccagcata cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat
    24121 acgcgtcgcc agtaaccaaa atgatgtcgc agctatccca gccaagttga tccatctctt
    24181 cacgagacat cggcaaaaac ggtgccggtc caaaacaggc ggcccagtac tgcggccagg
    24241 agaacaggtc gcgatccggt tggatcaggg agatagagct cattttgctt ccagaaatga
    24301 taaaaaaata atcaaaggcc ggggattata agccggaacg aaagagaaat cgaaaggtat
    24361 tccatactcg ccctcctcgg gcgagtatga agattacggt accggattga ccaacagttg
    24421 ccctatcgag ccacggtccg ccatttccag cgtctgactg ttgaagtaga acgggaagtg
    24481 cgcccaggaa ggctgaccga aataaaccag cagctccact tgtccgtcaa cccaaacggt
    24541 atctttccag cctctgtctt ccgggaacgg catcgcgcca ttcacgttac ggatctggaa
    24601 catcacgcct tcaatatgga acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca
    24661 cgttccttgc tgcgcggtga catcaatacg gttgacgtcc cacagttgtc cattaatacc
    24721 cgggtcatca cccagactga tatcgcgact gcgaatcggc gaaccggcca tgatttcagt
    24781 tggcagcagg cgcatcggaa gactgtctgt gaccagcggc agaaggccgg ttgggcgcag
    24841 cgttagcacc agggtagaaa ccagaatgct ggatggctca aagaagccgc gaatacgatc
    24901 aacaatgctc gccgcttcac cacaggtgat cgacacttca tcaccgttgc tcatatccac
    24961 cagaatctcg cggcgctcgc ccggtgccag cgaaagttgc ttcaccgata caggagcggg
    25021 caggaatccc tgatcgccag aaatcacatg taacgggcga ccatcgctca tctgtagttg
    25081 atagcgacga gagttcgacg cgttcaacag acgcaagcgc acccagccac gcgagacttc
    25141 aacgtacggg ctttgtacac cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc
    25201 cggttcgtta tattccggcg taccaaagtt atccagccgt ttatcctgaa taatgaccgg
    25261 aaaatcatcc acaccataat ggttggggat aggcagcgat ttgctgactt catcttccac
    25321 cagccacatt ccggcaaggc cgttatagac ctgctgagcc gtgcggttgg gagtattggc
    25381 gtgataccac agagtagctg cgttctggcg aatgggcagt acgggtgccc agtcagcgtt
    25441 tggcgacatc atccgtgccg gaccgcccat cagcgggcca ggtacctgta gcccggccac
    25501 cgtcattgag acattttctg tcaggcgatt gctgtaaata agcttaacat cgtcgccctt
    25561 ccagacgcgg atagtcggcc ccaggtaacg accattgatt ccccaaaccg atgcgcgtgt
    25621 ccctggcgta aatgaccagt gcgcacgttg tacagtcata aacagcggtt gcccacggcg
    25681 agattcaagc agcgtcggaa cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag
    25741 gggaacagcg cctgcacaaa gtgcaatccc cgatgcctga atgaactgac gccgactgag
    25801 tgacatattt gctccatgta aaactggcta aagtaatacc gtgcaggaaa gtgttccccg
    25861 caaaaataca gattgccgac ttaaactttt ccggcggctt cgcgctctgc gacttcttta
    25921 tcgagctcag cgattttttg ttccattatc gaacgacaat gggcagccaa ctcgcgaacc
    25981 tgatctttgc catactgact gacgtcaatt ggcggcagca tttcgacaat caccagaccg
    26041 ttgtgtagcc gattaagatt aatcttattc gaagttgtag agacgcacac gggaataatc
    26101 gggacgcccg ccgcaattgc cgcgtgaaat gccccggtct tgaacggcag caggccgcga
    26161 ccacggctgc gggttccttc cgggaacatc cagatggaaa tacggcgttt tttgaagtga
    26221 ttcacgactt ccgcaatagt gccgtgagct ttagtgcgat tgtttctgtc gatcaataag
    26281 ttgccggtta accagtacaa ctgcccgaag aaggggatcc acagcaagct ctttttccct
    26341 accgtcactg tcggcggctg cacaatgttc gatgccgtca ccatgtcata attgttctgg
    26401 tggttagcaa tatagatagc atttccgtag ctttcagcat ccgcaggttt gcggcactca
    26461 actttcaggc cgaacagcgg tgcaagacgg ccaaacatat gcccaaaggt ggccacatgt
    26521 ttcgggttac gcgggctgaa aaggcagtaa atggagccga atacacagac taagatgctg
    26581 taaatcacgg taataataag acgaaagata tatagcataa caccctctga aggtctgaga
    26641 gactggcatt gtacgtcacc tgaaatcagg ttagggcatt gttaaaagcc gctctctgga
    26701 aaagcgtatt gttaatcgca acgcatgaat aaacaacggt ttcaagggaa tttttatcgt
    26761 ttccgggggt tgaatacgct gccggatgac gacttaacgt ttcatccggc gttccttgca
    26821 agcgggagga aacagcgccc tccccggcat attactcttc gctatcaccg ctgctggcac
    26881 ggcgaggaga gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc
    26941 ggcgtccacg ttcgccagcg actttctgta gctcttccgg acgcagttta attttgcgtt
    27001 tcccaacatg aatggtcagc gtgctttgcg gcggcagaac gtacagttgc gcaagaccat
    27061 cctcgccacg cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg
    27121 acagctgcgg cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca
    27181 gcagcatatc ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta
    27241 aggtgatcaa agccttacct gcacggttac gcgccaccag atcgttaaag gtgcagacga
    27301 aaccgtaacc cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat
    27361 ggtcaacggt cgctccaggc ggcaacgtta atttgccggt gagcggctca ccctgaccac
    27421 gcgccgacgg cagcgtaatc gggtcgatgg catagctacg accggtggaa tcaacaaaca
    27481 ctaccggttg gttgctctta cctttcaccg ccgctttgaa gctatcgccc gctttataat
    27541 tcaggcccgg cgcgtcaata tcatggcctt tagcgctgcg cacccagccc atctgcgaca
    27601 gcacaatggt gacaggttca gacggcagca tgtcgtgctc gctcattgct ttcgcttctt
    27661 cgcgttcctg caacggcgaa cgacgttcgt caccgtaggc ttgcgcgtct gcctgcagtt
    27721 ctttcttcag caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc
    27781 gctctttttc cagctcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat
    27841 gacgcagttt cagttcgagg atcgcttccg cctgggtttc agtaaggcca aaccgcgaca
    27901 tcagcgccgg tttcggttca tcttcattac gaatgatctc aatcacttcg tcgatattga
    27961 gaaacgccac cagcaaacct tcgaggatat gcaggcgctt gaggactttc tccagacgat
    28021 agttcagtcg gcggcgcacg gtatcgcggc ggaacaccag ccattcggag aggatttcca
    28081 gcaggttttt caccgccgga cgaccatcca gaccgatcat attgaggtta atgcgatagc
    28141 tcttttccag atcggtggta gcaaagaggt ggttcatcac ctgatccata tccacgcggt
    28201 tggaacgcgg cacaatcacc agacgggtcg ggttctcgtg gtcagattca tcgcgcagat
    28261 cgtcaaccat cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg
    28321 cacctgaaac ctgatgcggc aatgcgctga taaccaccgc gccatcttct ttcttccaaa
    28381 ccgcgcgcat acgcactgaa ccacgtccgt tctcgtagat tttacgaatc tcggcgcgcg
    28441 aagtgataat ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgat
    28501 cgagcgtggt tttcggctgg tcgattaatg cgattgccgc ctgagccact tcacgcaggt
    28561 tatgcggtgg aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa
    28621 tgtttggcag acgggcaggc agcattttcg gctcctgcaa agtgccgtcg aagtttggca
    28681 cccagtcagc cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc
    28741 gggattcggt gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc
    28801 cctgaccatc aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt
    28861 cataacaggc actatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg
    28921 ccgatttttt aaatttggcg ctggcattca ggcccagctc agacatcgca tacacaatgc
    28981 ggcgctgaac aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc atgatcacgt
    29041 acatggagta gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca
    29101 tatcgctcat taattctgat tcctcaactt attcgccagc cttcaaaggg gcaatattgc
    29161 cggagatact acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc
    29221 ggacgtgctc cggcggcgat aaaggcatca atgtgccttg atatacaact ctttgctgta
    29281 gacataatcc tggggattat gcagcggaaa accgccaacg tagggtttaa gcagtttgat
    29341 taacggctga tagtagatgg gaatcagcgg tgcctgctgg ttgatgatca cttctgcctg
    29401 ctgatacaac gcattacgct ttgtcgcatc agtgatctgc gtggcctggt ttagtaaggc
    29461 gtcatactgc gcatttttcc agtgaccgac gttttcttca ctatcgcttt tgagcgtgtt
    29521 caggaagctg gaggcatcat tgtacgtcgc atcccacgac tgccgagaca gcataaaatc
    29581 accggctcgt cgggcatcaa gataggtttt ccactccatt gtgcgcagcg tcacctgtgc
    29641 acccagccat tttttccatt cggaagacaa cgctatcgcg gtcttttcat gcagatcgta
    29701 cttgttgtag aacagctcaa agcgtagcgg atgagaggcg tcgtatcccg cctgtttcag
    29761 caaggctttt gccatcgcga cacgctcact cattggcttt tgcagttcat cgaacgtcgt
    29821 cgcgctaaag ccttttacct ctggcggcgt cagcgtggtt gcgggcgttc tcaaccccag
    29881 taccttttgc gcaataagct gtcgatcaac cgtaagatat agcgcccgac gcacccgcac
    29941 atcgttaaat ggcggtttct caaggttaaa gttgtaatat tcgctgttca gacgcgggat
    30001 aattcgtagc tcgccaggca gtgatttttc aatggcggga atttgctgcg ccggaaccca
    30061 ggtgagatcg acctctcccg cgcgatagcg gttatagccg gtgaccgaat tatccagcgc
    30121 cagatactca acctgttgca ataccgtatg ttgcgcatcg cggtactttg gatttttgcg
    30181 tgcggtaatc ttttcgttaa ctacccattt atcaagcaca aaggcaccgt tgtaaaccat
    30241 gttctctggc ttactccagc tatcgccatg tttagcgatg acatgatgag gaaccgggaa
    30301 caacgtcggc caggcgagca tcgtcgtaaa ccaaggaact ggctgctcaa gcgtaacttc
    30361 aagagtacga tcatccgtcg ctttgatccc cagcgatgta acatctgctt tacccgcaac
    30421 aatagccgcg gcattgttaa tgtgcgcctg tgccagatag ccagcaaaag ggcttgccgt
    30481 tttcgggtca accgcacgtt gccagccgag gacaaaatcc tctgccgtca gaggctgacc
    30541 gtctgaccac tgcaaaccgc tacgcagatg gaaaatatag cgcttgccgc cgtcaagtat
    30601 ctcccagcgt tcagcctgag cgggctgcac ctggccttca ccgtccatcc ataccagacc
    30661 ttcaaacaga tccagcacaa tctgcgcggc agtattctcc tctacctttt gcgggtcgag
    30721 cgtgcctggg tcgctatgat tgttgtaacg aaagacttgt tgcggggcga gcggtgtgtt
    30781 ggcgggaacg tcagcagcgt agagaggagc cgcacttacc aggctgacca gccataataa
    30841 atttcgcgta tacatggtat tccttaccgg agaaattata agcccgacaa caatacgcct
    30901 gtggcattgt ttgctcaaac ttatcgcgag tgatttggct cacactccgg taaagattcc
    30961 ccatcacgcc aacggcacat aaatatccgt ttccagtcgc cgctctgtca tcccttcggc
    31021 aagattggtg tagtgaaaca ggatcggggc tttgcgcatt ttttcgccgc ttgcaggtag
    31081 ccagtggcga atgatgcccc atatcgtgtg tgaaatatcg tccagctcgc caacatggcg
    31141 ggcaacagca taacgcccac cggtaagttc gccattactc acgccataac gattatcggg
    31201 gatcggttcg ctaacgctgc cgcagatatc aaagcgaaac gcttctggtg cggtggtggc
    31261 tgggtcgtcc caggcgacgc caaaagtctg gctttggtta acaggagaaa gccccgtctc
    31321 tttacgccac atgataaatt tcgccacgct gtagttgagc aactcagggc tacagcggtg
    31381 cggcaacatc gccacaggga ttgatgggaa atcgataata tttacatcca gtgtcaggtt
    31441 tgtcattttg cctccctgcg aaattattcc cgggaacgcg tcccgggagc ggtaacaatt
    31501 acggatttac tttgcggatc tgtttgacgt caatttcaac agagttccag tctttatcga
    31561 cttccccctg aatctcaacc gtatctttcg gcgtcaccgt cacgccgttc cagcgtttat
    31621 ggtcgatatc aacattgata gtaccgctgg catctttgaa cacgtagaga tcgtcagaga
    31681 tgcgttcaac gatattgccg cgtagggtta cccaggtgtc gtcacgcagg gattttgcgc
    31741 tttctacagt cgttacgctg ccgttcggcc cctggaatcc tccggcctga ctttgcgttg
    31801 ccgatgggcc agaaaaacct ccctgctctg ctgccataac cggtgcactg cacagggcca
    31861 ttactgcgat tactgctgcg aattttttca tgtttattac tccctttaat gtctgtttcc
    31921 gagcatttaa caagatagtc cttaacaact tcttaaggga aaaaaataaa atttaatgct
    31981 gtacagagcg cgttacaaca cggtttactg gcagcaaata cggttatcgc agggatgaaa
    32041 aaatgcgaat tttactgata gaagatgaca tgctgattgg cgacggcatc aaaacgggcc
    32101 ttagtaaaat gggttttagc gtcgactggt ttacacaagg tcgtcaggga aaagaggcgc
    32161 tatatagcgc accttatgat gcggtgatcc tggatttaac cttaccgggc atggatggtc
    32221 gcgatatttt gcgcgaatgg cgagaaaaag gtcagcgtga gccggtattg atcctgactg
    32281 cgcgcgatgc gctggcggaa cgtgtagaag ggctgcgtct gggagctgac gattatctgt
    32341 gtaaaccttt tgcgttgata gaagtcgccg ccaggctgga agctctgatg cgccgaacca
    32401 acggccaggc cagcaacgag ctgcgccacg gcaacgtcat gctagacccc ggcaaacgta
    32461 tcgccacgct ggctggcgac cccttaacgc tgaaaccaaa agaatttgcc ctgctggaat
    32521 tactgatgcg taacgctggt cgggtactgc cgcgcaaact gattgaagag aaactgtata
    32581 cctgggacga agaggtcacc agtaatgccg ttgaagtgca tgtgcatcat ctgcgacgca
    32641 aactcggtag tgattttatt cgtaccgtgc atggtattgg ctacacatta ggtgagaaat
    32701 gaaatttacc caacgtctta gtctgcgagt caggctgacg ctaatctttt taattctggc
    32761 ctcggtgacc tggctgcttt ccagctttgt cgcctggaaa caaacaacgg ataacgtcga
    32821 tgagttgttc gacacccaac tgatgctgtt tgccaaacgg ttaagtacgc tcgatctcaa
    32881 cgaaatcaac gcggcggatc gcatggcaca gacgccaaat aaattaaaac acggtcatgt
    32941 tgatgacgat gcgctgactt ttgccatctt tacccacgac ggcagaatgg tccttaacga
    33001 tggcgataac ggagaagata ttccttatag ctatcagcgg gaaggttttg ctgacgggca
    33061 actggtcggt gaagacgatc cctggcgttt tgtctggatg acctcacctg atggcaaata
    33121 tcgcatcgtt gtgggtcagg aatgggaata ccgtgaagac atggcgctgg cgattgttgc
    33181 cggacaattg atcccgtggc tggtcgcact gccaattatg ttaatcatca tgatggtact
    33241 actgggtcgt gaactcgcgc cgctgaacaa actggcgctg gcactacgta tgcgtgaccc
    33301 tgactcggaa aaaccactaa acgcgacggg cgtacccagc gaagtgcgcc cactggttga
    33361 gtcgctaaat caactgttcg cccgcacaca tacgatgatg gttcgtgaac gacgctttac
    33421 ctccgacgca gctcacgaac ttcgtagccc gttaacggcg ctgaaagtgc aaaccgaagt
    33481 tgctcagctc tctgacgatg atccgcaggc gcggaaaaaa gcgctgcttc aattacattc
    33541 cggaatcgat cgtgctactc gtctggttga tcaactgctc acgctatcgc ggctggactc
    33601 actggataac cttcaggacg tcgcggagat cccgcttgaa gatctcctgc aatcgtcggt
    33661 gatggatatt taccacacgg cgcagcaggc gaacattgac gtgcgactga cactcaatgc
    33721 caacggcatc aaacgcaccg gacaaccgct attgctaagt ttgttggtgc gaaatttgct
    33781 ggataacgcc gtgcgctaca gtccacaggg cagcgtggtg gacgtcacgc tgaatgccga
    33841 taatttcatc gtgagggata acggccccgg tgtgacacca gaggcactgg cgagaattgg
    33901 cgaacgcttc tatcgcccac ccggacaaac cgctaccggc agtggacttg ggctatcaat
    33961 tgtccagcga atcgccaagc tgcatgacat gaatgttgaa tttgggaatg cggaacaagg
    34021 tggatttgag gcgaaagtaa gctggtaact tctctgtcta tgagagccgt taaaacgact
    34081 ctcatagatt ttattaatag caaaatataa accgtcccca aaaaagccac caaccacaaa
    34141 ccaaacaaat aacgagcgag attacatata accaaaactc aaacatttct cgca
//
LOCUS       DAZKJR010000038        33754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKJR010000038 DAZKJR010000000
VERSION     DAZKJR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.038"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            222..506
                     /gene="pduA"
                     /locus_tag="ACHOWS_001875"
     CDS             222..506
                     /gene="pduA"
                     /locus_tag="ACHOWS_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFQ0047451.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            503..1315
                     /gene="pduB"
                     /locus_tag="ACHOWS_001876"
     CDS             503..1315
                     /gene="pduB"
                     /locus_tag="ACHOWS_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFQ0047452.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1334..2998
                     /gene="pduC"
                     /locus_tag="ACHOWS_001877"
     CDS             1334..2998
                     /gene="pduC"
                     /locus_tag="ACHOWS_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFQ0047453.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            3008..3676
                     /locus_tag="ACHOWS_001878"
     CDS             3008..3676
                     /locus_tag="ACHOWS_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFQ0047454.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKPVSFRAPVSATASSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3691..4209
                     /gene="pduE"
                     /locus_tag="ACHOWS_001879"
     CDS             3691..4209
                     /gene="pduE"
                     /locus_tag="ACHOWS_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFQ0047455.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4219..6051
                     /locus_tag="ACHOWS_001880"
     CDS             4219..6051
                     /locus_tag="ACHOWS_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFQ0047456.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSYVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     LGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKGAIHGK"
     gene            6041..6391
                     /locus_tag="ACHOWS_001881"
     CDS             6041..6391
                     /locus_tag="ACHOWS_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFQ0047457.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6411..6686
                     /gene="pduJ"
                     /locus_tag="ACHOWS_001882"
     CDS             6411..6686
                     /gene="pduJ"
                     /locus_tag="ACHOWS_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFQ0047458.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6710..7132
                     /locus_tag="ACHOWS_001883"
     CDS             6710..7132
                     /locus_tag="ACHOWS_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFQ0047459.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            7135..7767
                     /locus_tag="ACHOWS_001884"
     CDS             7135..7767
                     /locus_tag="ACHOWS_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFQ0047460.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVTVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVT"
     gene            7764..8255
                     /gene="pduM"
                     /locus_tag="ACHOWS_001885"
     CDS             7764..8255
                     /gene="pduM"
                     /locus_tag="ACHOWS_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFQ0047461.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHDRLLSYVDIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8259..8534
                     /locus_tag="ACHOWS_001886"
     CDS             8259..8534
                     /locus_tag="ACHOWS_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFQ0047462.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRQVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8546..9553
                     /gene="pduO"
                     /locus_tag="ACHOWS_001887"
     CDS             8546..9553
                     /gene="pduO"
                     /locus_tag="ACHOWS_001887"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFQ0047463.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHCFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9550..10935
                     /gene="pduP"
                     /locus_tag="ACHOWS_001888"
     CDS             9550..10935
                     /gene="pduP"
                     /locus_tag="ACHOWS_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFQ0047464.1"
                     /translation="MNNSELETLIRNILREQLVPATQRAQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITIIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VVSVAEYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10946..12058
                     /locus_tag="ACHOWS_001889"
     CDS             10946..12058
                     /locus_tag="ACHOWS_001889"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFQ0047465.1"
                     /translation="MKTFSLQTRLYSGTGSLNILNRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIHFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            12055..13398
                     /locus_tag="ACHOWS_001890"
     CDS             12055..13398
                     /locus_tag="ACHOWS_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFQ0047466.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVNVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGNLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13401..13955
                     /gene="pduT"
                     /locus_tag="ACHOWS_001891"
     CDS             13401..13955
                     /gene="pduT"
                     /locus_tag="ACHOWS_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFQ0047467.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13955..14305
                     /gene="pduU"
                     /locus_tag="ACHOWS_001892"
     CDS             13955..14305
                     /gene="pduU"
                     /locus_tag="ACHOWS_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFQ0047468.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14310..14753
                     /locus_tag="ACHOWS_001893"
     CDS             14310..14753
                     /locus_tag="ACHOWS_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFQ0047469.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(14854..15183)
                     /gene="tmaR"
                     /locus_tag="ACHOWS_001894"
     CDS             complement(14854..15183)
                     /gene="tmaR"
                     /locus_tag="ACHOWS_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFQ0047470.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15355..16413)
                     /gene="yeeA"
                     /locus_tag="ACHOWS_001895"
     CDS             complement(15355..16413)
                     /gene="yeeA"
                     /locus_tag="ACHOWS_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0047471.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16611..17084)
                     /gene="sbmC"
                     /locus_tag="ACHOWS_001896"
     CDS             complement(16611..17084)
                     /gene="sbmC"
                     /locus_tag="ACHOWS_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFQ0047472.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17203..18369)
                     /gene="dacD"
                     /locus_tag="ACHOWS_001897"
     CDS             complement(17203..18369)
                     /gene="dacD"
                     /locus_tag="ACHOWS_001897"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFQ0047473.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVAMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18578..20005
                     /gene="sbcB"
                     /locus_tag="ACHOWS_001898"
     CDS             18578..20005
                     /gene="sbcB"
                     /locus_tag="ACHOWS_001898"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFQ0047474.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSKFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(20079..21437)
                     /gene="plaP"
                     /locus_tag="ACHOWS_001899"
     CDS             complement(20079..21437)
                     /gene="plaP"
                     /locus_tag="ACHOWS_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFQ0047475.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(21427..21489)
                     /gene="yoeI"
                     /locus_tag="ACHOWS_001900"
     CDS             complement(21427..21489)
                     /gene="yoeI"
                     /locus_tag="ACHOWS_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFQ0047476.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(21704..22633)
                     /gene="yeeY"
                     /locus_tag="ACHOWS_001901"
     CDS             complement(21704..22633)
                     /gene="yeeY"
                     /locus_tag="ACHOWS_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0047477.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(22679..23503)
                     /gene="yeeZ"
                     /locus_tag="ACHOWS_001902"
     CDS             complement(22679..23503)
                     /gene="yeeZ"
                     /locus_tag="ACHOWS_001902"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0047478.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(23586..23840)
                     /gene="yoeB"
                     /locus_tag="ACHOWS_001903"
     CDS             complement(23586..23840)
                     /gene="yoeB"
                     /locus_tag="ACHOWS_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFQ0047479.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(23837..24088)
                     /gene="yefM"
                     /locus_tag="ACHOWS_001904"
     CDS             complement(23837..24088)
                     /gene="yefM"
                     /locus_tag="ACHOWS_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFQ0047480.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            24371..24421
                     /gene="hisL"
                     /locus_tag="ACHOWS_001905"
     CDS             24371..24421
                     /gene="hisL"
                     /locus_tag="ACHOWS_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFQ0047481.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    24397..24520
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            24567..25466
                     /gene="hisG"
                     /locus_tag="ACHOWS_001906"
     CDS             24567..25466
                     /gene="hisG"
                     /locus_tag="ACHOWS_001906"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFQ0047482.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            25472..26776
                     /gene="hisD"
                     /locus_tag="ACHOWS_001907"
     CDS             25472..26776
                     /gene="hisD"
                     /locus_tag="ACHOWS_001907"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFQ0047483.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            26773..27843
                     /gene="hisC"
                     /locus_tag="ACHOWS_001908"
     CDS             26773..27843
                     /gene="hisC"
                     /locus_tag="ACHOWS_001908"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFQ0047484.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            27843..28910
                     /gene="hisB"
                     /locus_tag="ACHOWS_001909"
     CDS             27843..28910
                     /gene="hisB"
                     /locus_tag="ACHOWS_001909"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFQ0047485.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDLANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            28910..29500
                     /gene="hisH"
                     /locus_tag="ACHOWS_001910"
     CDS             28910..29500
                     /gene="hisH"
                     /locus_tag="ACHOWS_001910"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFQ0047486.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLARRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            29500..30237
                     /gene="hisA"
                     /locus_tag="ACHOWS_001911"
     CDS             29500..30237
                     /gene="hisA"
                     /locus_tag="ACHOWS_001911"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFQ0047487.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPARRQIPLIKTLVADVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            30219..30995
                     /gene="hisF"
                     /locus_tag="ACHOWS_001912"
     CDS             30219..30995
                     /gene="hisF"
                     /locus_tag="ACHOWS_001912"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFQ0047488.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            30989..31600
                     /gene="hisIE"
                     /locus_tag="ACHOWS_001913"
     CDS             30989..31600
                     /gene="hisIE"
                     /locus_tag="ACHOWS_001913"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFQ0047489.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(31695..32672)
                     /gene="wzzB"
                     /locus_tag="ACHOWS_001914"
     CDS             complement(31695..32672)
                     /gene="wzzB"
                     /locus_tag="ACHOWS_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFQ0047490.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(32819..>33754)
                     /locus_tag="ACHOWS_001915"
     CDS             complement(32819..>33754)
                     /locus_tag="ACHOWS_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0047491.1"
                     /translation="IIATPTDYDPKTNYFNTSSVESVIKDVVEINPYAVMVIKSTVPV
                     GFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVIGERSERAERFAALLQEGAI
                     KQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYAESLGLNTRQIIEGVCLDPR
                     IGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAIVDANRTRKDFIADAILSRK
                     PQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDL
                     ATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         8579 a         8747 c         8517 g         7911 t
ORIGIN      
        1 tcaacgaaat attactcgcg attaaactca ctagccaacg caattaattc acattgaaat
       61 atctacaaaa caaccaataa attaacaaca tatagttcat aaaagcaatt ttattatatt
      121 tgttctctat taatttttct cattagccga ttaagtcagc cattcttttt cctcgctttt
      181 ttttatagtc atatcgtcaa aaaaatccag gcgaggtctt tatgcaacaa gaagcgttag
      241 gaatggtaga aaccaaaggc ttaactgccg ccatagaggc cgcagacgcc atggtgaagt
      301 cggcaaatgt tttgctggtc ggctacgaaa aaataggttc cgggctggtg accgtcatcg
      361 ttcgtggtga tgtcggggcg gtaaaagcgg caacagacgc gggtgccgca gctgcgcgta
      421 acgtcggcga agtaaaagcc gtacacgtta ttccccgccc acataccgat gtcgaaaaaa
      481 tcttaccaaa gggaattaac ccatgagcag caatgaactg gtcgaacaga tcatggcgca
      541 ggtcatcgct cgcgtagcga cgccagagca tgaggttctc ccgaaaacca ctcatcaaat
      601 acgagagacg gctatggcag aaaaaagctg cacgttaaca gaatttgttg gcaccgctat
      661 tggcgacact gtaggtctgg tcattgcgaa cgttgatagc gccctgttgg acgcgatgaa
      721 acttgaaaaa cgctatcgtt ccattggcat tctgggggcg cgtattggcg cagggccaca
      781 cattatggcc gctgatgaag cggtaaaagc caccaacact gaagttatta gcattgaact
      841 tcctcgggat actaaaggcg gtgccggaca cggctcattg atcctcttag gtggcaacga
      901 tgtttccgac gtgaaacgcg gtattgaagt tgccctgaaa gaactcgatc ggacctttgg
      961 tgatgtctat gccaacgaag ccgggcatct tgaactgcaa tacaccgccc gcgccagcta
     1021 tgcactggaa aaagcgtttg gcgctccact gggacgggct tgcggcgtta ttgtcggcgc
     1081 tccggctgcg gtgggtgttc tgatggccga caccgcgctg aagtccgcca atgtggatgt
     1141 cgtggcatac agctcccccg ctcaaggcac cagctacagc aacgaagtca ttctgattat
     1201 ctcgggtgac tccggcgcag tccgccaggc ggtcatctcc gcacgcgaaa tcggtaaaac
     1261 cgtgctttcg actctcggcg atacaccgaa aaacgatcgc ccttcctaca tctgaattct
     1321 ctgaggcaga tttatgagat cgaaaagatt tgaagcactg gcaaaacgcc cggtcaacca
     1381 ggacggtttc gttaaagagt ggattgaaga aggcttcatt gcgatggaaa gcccgaacga
     1441 tcctaaaccg tcaatcaaaa ttgttaacgg cgtcgttacc gaactggatg gaaaacccgc
     1501 caatcagttt gacctgatcg accactttat cgcccgctac ggcatcaatc tcgcgcgcgc
     1561 agaagaagtc atcgcgatgg attcggtaaa acttgccaat atgttgtgcg atccaaatgt
     1621 caaacgtagc gatatcgttc cccttaccac agcaatgacg cctgcaaaaa tcgtcgaagt
     1681 ggtttcgcag atgaacgtgg tagagatgat gatggcgatg cagaaaatgc gcgcccgtcg
     1741 aacaccatca caacaggctc acgtcaccaa catcaaagat aacccggtac aaattgccgc
     1801 cgacgcagct gaaggcgcat ggcgcgggtt tgatgaacag gaaaccaccg ttgccgttgc
     1861 tcgctatgca ccgttcaacg ccatcgccct gctggtcggc tcacaggtgg gacgtccggg
     1921 cgtcttgacc cagtgttcac tggaagaagc caccgaactg aaactcggta tgttaggcca
     1981 cacctgttac gccgaaacaa tttctgtcta tggtacagaa cctgttttca ccgatggcga
     2041 tgataccccg tggtcaaaag ggtttctcgc gtcatcctat gcgtctcgcg gcttaaaaat
     2101 gcgctttact tccggctccg gctctgaagt acagatgggt tacgcggaag gcaaatcaat
     2161 gctttatctc gaggctcgat gcatttacat caccaaagcc gcaggcgttc agggtctgca
     2221 gaatggttcg gtaagctgta tcggtgttcc atcagctgtg ccatcaggca tccgcgcagt
     2281 attagcggaa aacttaattt gctcatcgct ggatctggaa tgcgcctcca gtaatgacca
     2341 gacctttacc cactcggata tgcgccgtac cgcacgtttc ctgatgcaat ttctgccagg
     2401 taccgacttt atctcttccg gtttttccgc tgtgccgaac tacgacaaca tgtttgctgg
     2461 ttcaaacgaa gatgctgaag acttcgatga ttacaacgtc attcagcgtg acctgaaggt
     2521 tgatggcggc ctgcgaccag tacgcgaaga agatgttatc gctatccgca ataaagccgc
     2581 tcgcgcttta caggctgtct ttgccggtat gggactaccg cctattaccg atgaagaagt
     2641 cgaagcggca acctatgccc acggttcaaa agatatgcct gagcgcaata ttgtagaaga
     2701 catcaagttc gcccaggaga ttatcaataa aaaccggaac agcctggaag tggtgaaagc
     2761 cctggcgcaa ggcggtttta ccgatgtcgc ccaggacatg ctcaacatgc aaaaagcaaa
     2821 actgaccggc gattatctcc atacctcagc catcatcgtc gatgacggac aagtgctctc
     2881 tgcggtcaat gacgtcaatg attacgccgg accggctaca ggttaccgcc tgcaagggga
     2941 acgctgggaa gagatcaaaa atattcccgg tgcacttgat cccaacgaaa ttgactaagg
     3001 gtgagaaatg gaaattaacg aaaaattact gcgtcagatt atcgaagacg tgctggcaga
     3061 aatgcaaacc agcgacaagc ccgtttcatt tcgcgcacct gtctctgcaa ctgcctcttc
     3121 cgcgccagat accggaaatt ttctgaccga aattggcgaa gcccaacaag gcactcagca
     3181 agatgaagtc attattgctg taggcccggc gttcggcctg gcgcaaaccg tcaatatcat
     3241 cggtattccg cataaaaaca ttttgcgcga agtgattgcc ggtatcgaag aagaaggcat
     3301 taaagcccgt gtgattcgct gctttaaatc gtccgatgtc gcattcgtag cagtcgaagg
     3361 cgatcgtctt agtggttcag gtattgctat cggtattcag tcgaaaggca caacggttat
     3421 ccaccagcag ggacttcctc ccttgtctaa cctggaactt ttcccacaag cgcctttgtt
     3481 gacgctggat acttatcgtc agattggcaa aaacgccgcg cgttatgcaa agcgtgagtc
     3541 tccacaaccg gtgccgaccc tcaacgatca aatggcgcgg ccgaaatacc aggccaaatc
     3601 tgccattttg catattaaag agacgaagta cgtggtcacc ggcaaaaaac cgcaagaact
     3661 gcgcgtgacg ttttaacaaa ggaaaccttc atgaataccg atgcaattga gtcgatggtc
     3721 agagacgtgt tgaaccgcat gaacagttta caggatgcag ctcctgtttc tgcggtcccc
     3781 aacgcgtcaa tacagagcgc caaagtgacg gattacccgc tggcaaataa acatccggaa
     3841 tgggttaaaa cggcgaccaa taaaacgctc gacgatttca ctctggaaaa tgtgctaagc
     3901 gataacgtca ccgcccagga tatgcgtata acccctgaaa cattgcgtat tcaggccgca
     3961 attgccagag atgctggtcg cgatcgcctg gcgatgaatt ttgaacgcgc cgctgagctt
     4021 acctcagtac cggatgatcg catcctcgaa atctacaatg cgttacgccc ttatcgttcg
     4081 actaagcaag agcttattgc cattgctgac gatcttgaac ggcgttatca ggccaaaatt
     4141 tgtgctgcat ttgtccgtga agctgcggaa ctttatgtcg agcgcaaaaa actgaaaggc
     4201 gacgattaag gacgaactat gcgatatata gcaggcattg atatcggcaa ctcatcaaca
     4261 gaggttgcgc tggcaacgct cagtgcaact ggagagttgt cgtacgtgag tagcgccctg
     4321 gcggaaacca ccggaattaa aggtacgttg cgtaacgtcc atggtattca ggaggcgctc
     4381 gcacaggcaa cgaaaaaagt cggcatcaat gttagcgata tttcgcttat tcgtatcaac
     4441 gaagcaactc cggtcattgg tgatgtggcg atggaaacca tcactgaaac catcatcacc
     4501 gaatcaacca tgatcggtca caacccgaaa actccgggcg gagttgggct gggcgtaggg
     4561 ctcaccatca ctccgcaaga gttattaaca cgcccggcgg acacacctta catcctggtg
     4621 gtgtcatcgg catttgattt tgccgatatc gcaacgatga taaacgcctc cgtacgagcc
     4681 ggatatcagc tgactggcgt tattctgcaa cgcgacgatg gcgtactggt caataaccgc
     4741 ctggaaattc ctctccctat tgtggatgaa gtcctctaca tcgatcgtat tccgctcggc
     4801 atgctggctg caattgaagt tgccgttccc gggaaagtta tcgaaaccct gtctaacccg
     4861 tatgggattg ccaccgtttt cgccctcaat gctgaagaga ctaaaaatat tgttcctgtc
     4921 gcgcgggcgc taatcggcaa ccgttcagca gtggtagtga aaacgccttc tggcgacgtt
     4981 aaagcccgtt cgatacccgc cggaaatatc gaactgcttt ctgccgggcg cacaacgcgg
     5041 gttgatgtag ctgctggtgc cgatgccatt atgaaagcgg tcggtgagtg cccaaaactg
     5101 gaaaacgtga cgggagaacc tggctccaac atcggcggaa tgctggaaca tgttcgccag
     5161 accatggctg aactgaccaa caaacccagc aacgaaattt ttattcagga tttgctggcg
     5221 atagatacct ctgtccccgt caacgtaacc ggtggtctgg caggtgaatt ttcactggaa
     5281 caggctgtgg gtattgcctc tatggtgaaa tccgaccgtc tgcaaatggc gatgatcgcc
     5341 agtgaaatta agcaaaaact gcatgttgat gttcaggtcg gaggcgcaga agcagaagcc
     5401 gccattcagg gggccttgac tacccctgga accacacgtc cactggcaat tctggactta
     5461 ggtgcaggct ctaccgatgc ctccatcatc aatcagaaag gggaaatagt tgccactcac
     5521 cttgcgggag cgggcgacat ggtaacgatg attatcgctc gtgagctggg cttaaatgat
     5581 cgttatctcg cagaagaaat caaaaagtac ccgttggcaa aagtagaaag cctgttccac
     5641 ttacgccatg aagatggcag cgttcagttc ttccctactc cgctgtcccc tcatgtattt
     5701 gcccgcgttt gtgtggtgaa acctgacgaa ctggtgccta ttctcggtga tttgacgctg
     5761 gaaaaagtac gtgctgtccg tcgtagtgcg aaagagcgtg tcttcgtaac taatgccctg
     5821 cgcgcgctac gtcaggtaag ccctgccgga aatattcgcg acattccgtt cgtcgttctg
     5881 gtgggtggtt cttcgctgga tttcgaagta ccgcaattgg tcaccgacgc gctggcacat
     5941 tatcgactgg tcgccgggcg ggggaatatc cgtggtagcg aaggtccgcg caatgcggta
     6001 gctactgggc tcctcatcgc ctggcacaag ggggcgattc atggaaagta aagagcgtgc
     6061 accagccatc gttgtcatgg agatcggtga ctgtatcact acatgggatg aagtgcttct
     6121 tggcattgaa gaggaaggta ttccctttcg cattcaacac attccgtcag gtgaagttat
     6181 cgatagcgcc tggctggcgg ctcgtcaatc tcccctgttg gttggcattg cctgcgatca
     6241 ggaaaaactg attgtgcatt acaaaaactt accggcatca gcgccactgt ttacgctgat
     6301 gtatcaacaa gataaccatg ccaggcgcag catcggcact aacgctgccc gcctggtaaa
     6361 agggattccc ttccgggaac tccactcata atccacagga gaaacgcagt atgaacaacg
     6421 cactaggact ggttgaaaca aaagggttag ttggcgcaat tgaagctgca gatgccatgg
     6481 ttaaatccgc caacgtgcag ctggttggct atgaaaagat tggttcgggt ctgattacgg
     6541 tgatggtccg tggcgatgtt ggcgcagtaa aagccgccgt tgatgcagga agcgccgccg
     6601 caagcgccgt tggcgaagtg aaatcctgcc atgtcatccc gcgcccacac agtgatgttg
     6661 aggctatttt acctaaatca gcataacccc attcgctaag gagcacagcg tgaagcaatc
     6721 actgggatta cttgaagtca gtggtctggc actcgccatc agttgcgcag atgctatggc
     6781 aaaagccgcg tccatcacgc tggttggcct tgagaagacc aatggctcag gctggacgct
     6841 catcaaaatc accggtgacg tcgcctcggt taatgcggcg atcgcgacgg gagccagttt
     6901 cgccgggcag cgaaatggcc ttgtcgcaca caaagtaatt gcccgcccgg gagacggcat
     6961 tctgccccac acaactgaag cattgactgt gacggttgaa acaagcgaaa caccagcaac
     7021 aaccgtcatc tcagagatta tcagttgtaa tttgtgtctt gacccggcat gtcagcggca
     7081 aaaaggtgag ccccgctcac tttgcctgta cgccaataag cgaggggagt agcagtggat
     7141 aaacaaaccc tgcaggaaac agtgagccag attctggatg agatgcgcga gcgccctatc
     7201 ccactgggcg tttccagtcg ccatattcat ctttcaacag cggattattt gcgtcttttt
     7261 cctgaacaac cgattagcga gaaaaagccg ttaatgcaac cgggccaatt cgctgctgaa
     7321 caggttgtta cgctggaggg accaaaaggc aaattgcaca acgttcgctt gttggggccg
     7381 ctacgcaatg caagccaggt ggaaatttcc cggacggacg ccagaacgtt agggatcacc
     7441 gccccgctac gcatgtcagg cgatctcagc ggcactccgg ggattcgttt gataagccca
     7501 tttgcggaac tggagatcac atctggcgtc attgtcgcgc aacggcatat tcatatgtct
     7561 ccgctggatg ccctgattta tggtgttacg cacggcgata aagtggcggt tgccattgaa
     7621 ggtgatgagc gacgactgat attcgaaaac gtcactgttc gcgtctctcc ggatatgcgt
     7681 cttgaaatgc atattgatac cgatgaagcc aacgccgcag gggctgatag ccctgacgtt
     7741 tttgccacac tggtgtcagt gacatgaata acatgctgat gcaacgtatt gttgacgaag
     7801 tggtttttcg ccttaaacaa cgtgcgggaa aaacactcgt gctgactgtt tttcaactgc
     7861 gagacgccag cgttcaagag agcgttcatc agtatgcgtc attacaaatc cgatatgtcg
     7921 atctgccgct gctgcgacaa cttgcggaga acgaaacgtc agatcgagca gcaatccaga
     7981 tacacgaagc gttagcctgg ggaatgcaca ttcaattgtc gttgcagcgt catttcctga
     8041 acgccattga attgaagact ctcgcccgtc taccgctaag ctggtgcgac gaacaagggc
     8101 aaccgatcta cctacatcat gatcgacttt taagttatgt cgatatcgca cagttaagct
     8161 cgggaatatt ggtgttacag cggaaatgtt gtgtgaccgc actggctcgt gaagcggcaa
     8221 ttacccgaaa tattcagtta atcaggcagg agtaacctat gcatctggct cgagtcactg
     8281 gcgcggtagt atccacgcaa aaatcacctt ctctgatcgg gaaaaaactg ctgctggttc
     8341 gccaggtcag tgctgacggc gaactgcctc tcacgccatt aaccggcgac gaagttgccg
     8401 ttgattccgt cggcgcaggc gttggtgagt tggtactcct cagcagtggt tccagcgcca
     8461 gacatgtgtt ttccggccct aacgaagcca tcgacctggc agtggtcggc attgtcgata
     8521 cgctttcgcg ctaaggacct gaaagatggc aatttatacc cgaacaggtg attcaggaag
     8581 tacgtcttta tttaccggac aacgcgtcag caaaactcat ctacgtgtcg aaacctatgg
     8641 cacgctggat gaactgaacg cgacactcag tctgtgttat tgcgccaccg ccatcgaaag
     8701 ccatcgcatc ttactggaag ccattcaaca acagatattt tggtttagcg ccgaactcgc
     8761 cagtgaaagc gaacagccgt cagcgcagca gcgttacatc ggtacggaag aaattgccgc
     8821 gctggaaaac gcgatcgata gcgcaatgaa cgccgtcccc ccggttcact gcttcattct
     8881 gcctggtcga tgtgaagccg caagccgcat gcatttcgcc cgcacggtgg ctcgacgggc
     8941 tgaaagacgt ctggttgaac tgacaacaga aaccaccgtc cggaatgttt tgctgcacta
     9001 tatcaaccga ctttccgatt gcctgtatgc cctcgcccgc gtggaagaca acgttgctca
     9061 tcaaaacctg atgattcagg aaatcacaaa acgttatcac gaagccaacc acacaccagc
     9121 attgaaggaa cgcacaatgc cgctcacttt tcaggatctt caccagctca ttcgtagtgc
     9181 ggcaatgcgc gcagatgaac tgcatattcc cgttgtcatc agcattgtcg atgccaacgg
     9241 aacagaaagt gttacctggc ggatgcctga tgccctgttg gtcagcagtg aactggcacc
     9301 gaaaaaagcc tggaccgccg tggcgatgaa aacggcaacc cacaagcttg ctgataccgt
     9361 gcaacccggc gcgccgcttt atgggctcga aagccatatg caaggcaaag tcgtcacctt
     9421 tggtggcggc ttccccctct ggcgtgacgg aaaattgctt ggcgggcttg gcatcagcgg
     9481 cggtagcgtt gaacaagaca tggatattgc ccaaagcgca atggcggcaa ttaacgtggg
     9541 agtaaaccaa tgaataactc tgaattagaa acactgatcc gcaatattct gcgtgagcag
     9601 cttgtccccg ctacgcaacg agcgcaaaga aacgctattt tccagactgt tgatgaagct
     9661 gtctgcgcag cacaccaggc ctttttacgc tttcaacagt gcccgcttaa aacccgtagc
     9721 gccattatca gcgccattcg cgaagaatta atgccccgcc ttaccgaact ggcagaagaa
     9781 agcgccaaag aaacggggat gggaaataaa gaagataagt acctaaaaaa caaagcggca
     9841 cttgataaca cgcctggaat tgaagattta accaccacag cactgaccgg tgatggaggc
     9901 atggttttgt ttgaatactc tccgtttggg gtgattggct cggtcacgcc aagcaccaac
     9961 cccacggaaa ccatcattaa caacagcatc agcatgctgg cagcaggtaa tagcgtctac
    10021 ttcagtccac acccgggagc caaaaacgtt tcgttaaaac tgattaccat aatcgaagac
    10081 atcgcgtttc gctgtagtgg cattcacaac ctgatcgtta ccgtatctga accaaccttt
    10141 gccgcaaccg aacaaatgat ggcgcaccct aaaatcagcg ttctggcgat taccggtggc
    10201 ccggggatcg tcgccatggg aatgaaaagt ggcaagaaag tgattggtgc cggagcgggt
    10261 aacccaccct gcattgtcga tgaaacggcg gatattgtga aagccgccga ggacatcatc
    10321 aacggcgctt catttgatta caacctgccg tgtatcgctg aaaaaagcct gattgtcgtg
    10381 gtatcagtgg cagagtattt aattcagcag atgcaaagct ttggagcact tcttcttaac
    10441 catgaacaaa cggagaaatt gcgtgcaatt tgcctgccag acggtagcgc caacaaaaaa
    10501 ctggttggga aaagcccatc agccttgctg gaagcagcgg gcctcccttt gcccgcaaaa
    10561 gcgccgcgcc tactgattgc tgttgttgac gccgatgata gttgggttac gtgtgaacaa
    10621 ctgatgccaa tgctgcccat tgtgaaggtc aacgattttg acagcgctct cgccctggca
    10681 ttgaaagtgg aagacggctt gcaccacacg gcaattatgc actcgcagaa cgtctcacgg
    10741 ctcaaccttg ccgcgcgcgt tatgcaaacc tcaatctttg tcaaaaacgg cccctcctgg
    10801 gcgggtattg gcgtcggcgg cgaaggcttt acgaccttta ccattgcaac accaaccgga
    10861 gagggtacaa cgtcagcacg gacttttgcc cgctcacgac gctgcgtgtt aaccagcggc
    10921 ttttccattc gctagagaaa cggaaatgaa aactttttca ctgcagacgc gtttatacag
    10981 cggaacggga agtctcaaca tactgaaccg tttcacgaat aggcacatct ggattatttg
    11041 cgacgtcttt ctggcacact caccactcat tgacacgttg catcaggcgc tgcccgacag
    11101 taatcgtctc agtattttta gcgaaataac gcccgaccca actatccaga ccgtggtgaa
    11161 aggcattgcg caaatgcaga ctttacgccc ggatgtggtt atcggttttg gcggcggttc
    11221 tgccctggac gccgcaaaag cgattgtctg gttcggtcgc cagtgtggca ttgaaattga
    11281 aacctgcgta gccattccta ccaccagcgg taccggttcc gaagtgacca gtgcttgtgt
    11341 catcagcgat cctgagaaag ggattaaata tccccttttc gataatgcac tttatccgga
    11401 tatcgcgatc cttgatccat ctttggtcgt cagcgtgcca ccggcgataa cggccaatac
    11461 cgggatggat gttttaaccc atgcgcttga agcttatgtt tctccccgcg ccagcgactt
    11521 cacggatgca ctggtcgaaa aagccgtgca aattgttttt caatacctgc ccactgcagt
    11581 gaagaaaggc gattgtcttg ccacccgtgg caaaatgcat aatgcatcga cgcttgcagg
    11641 aatagccttt agccaggccg gactcggttt aaaccatgcc attgctcatc aactcggcgg
    11701 acagtttcat ctgccgcacg gcctggcgaa tgcgctcctg ctcacggcgg ttatccactt
    11761 taatgctggc gatccgcgcg cagccaaacg ctacgcccgg ctggcgaaaa cgtgccattt
    11821 atgcccggac aacgccaatg acaccgccag cctgaatgca cttattcaac atattgagca
    11881 gcttaaaaca acctgtacgc tgccgactct taccaatgcg ctgaaagaaa aaaaagcaga
    11941 atggtcaata cgcataccag atatggttca ggcggcactg gcggatgcaa cgttgcgtac
    12001 caacccgcgt gcggctgatg cctccgctat tgcagaactg ctcgaggagt tgttatgaac
    12061 gtcgagttaa cctcctgcga cgaccagacc atccgcgagc gagtacaggc agcaggtgtc
    12121 gtgggtgctg gcggcgcagg ttttccaacc catattaagc tacaagcgcg tgttgacact
    12181 tttctggtga atgcggcgga atgcgaacca atgttgaaag tggatcaaca gttaatggtg
    12241 cagcaagcca gccgcctgct tcgcggtgtt cactatgcga tgaaagccac cggggccagt
    12301 agcggaatca tcgcgctgaa agagaaatat caacgggcaa taaacgcact caccccccta
    12361 ttaccaccgg atatccgcct gcatattttg cccgacgttt accccgcagg agacgaagtg
    12421 ctgaccatct ggatggcaac tggtcgccgc gttccacccg ctgcattacc cgttaacgtc
    12481 ggtgtggtgg tcaataatgt tcaaaccgta cttaatattg ccagagccgt cgagcaacag
    12541 tatccagtga cgcgccgcac actcactatt aatggtgcag tggcgaaacc aataacggta
    12601 accgtcccca tcggcatgtc tttacgtgaa gtcctggcgc tggcgggcgg cgcaacagta
    12661 gatgctcctg gttttatcaa tggcgggccg atgatgggaa atttgatcac atcgctcgac
    12721 acccccgtca gcaaaaccac tggcggcttg ctggttttac ccaactctca ttcactgatc
    12781 caacgtcgaa tgcagaatga ccgcagcgta cttaccgtcg cgaaaactgt ttgtgagcaa
    12841 tgccgcttgt gtaccgatct ctgcccacgt catttagtcg gtcacgaact ggcaccccat
    12901 ttattagtac gtgcggttaa ctaccaacag ctcgctacgc cacaactgtt actgacggcg
    12961 ctcacctgtt cggagtgcaa cgtctgcgcc agcgtcgcct gtccggtagg aatttcacca
    13021 atgcgcataa accgcctgct taagcaggaa ttacgcgcac aaaatctccg ctacgaaggt
    13081 gccttaaatc ctgctgatcc catggcaaat tatcgactga ttcccgtaaa acggctggta
    13141 accagactgg gcctaatccc gtggtatcag gatgcgccat tgtctgaaca ggtgcctcag
    13201 cccgaaaaag tgacgttatt gttacgccag cacatcggtg ccagcgcaat tgcgtgtgta
    13261 caaaaaggcg atcgcgtcgt tcatgggcaa tgtgtaggtc aaattccaca cggcacatta
    13321 ggtgccccca ttcatgccag tattgatggc atggtcagcg atgtgacaga aaacgcgatt
    13381 acgcttgtga gaggttaacg atgtcgaaag cggtaggtat tttagaactt agcagtatcg
    13441 cgaaagggat ggaaaccggc gacgccatgc tgaaaagcgc aaacgttgag ttactggtca
    13501 gcaaaacgct atgccccgga aaatttctgc tgatgctcgg tggcgatgtg ggtgcggtgc
    13561 aacaggccat caacagcggt gcttcccttg ctggtgaaat gctggtcgac agtcttgtcc
    13621 tgccaaatat tcatcccagt ctcctttctg caatcagcgg actgaaccat gttgaacagc
    13681 accgggctgt cggcgtagtc gaaacctgga gtgtcgccgc ctgcattgac gctgccgatt
    13741 gtgctgtcaa agctgcgaat gtcattctcg ttcgcgtaca tatggctttt ggcattggtg
    13801 gtaaatgcta catggtggtt gctggcgata tttcagatgt taataacgcg gtctctgtcg
    13861 ccagcgagcg tgcgggggaa aaaggtttgt tggtctatcg gtcagtgatt gcccgtccac
    13921 atgaagccat gtggcgtcag atggtggagg gataatgaca actcagcaca caactgaacg
    13981 gatgattcag gaatatgtcc ctggtaagca ggtcacgctg gcacatgtca tcgccaatcc
    14041 tgggaaggat ctgtttaaga aattgggttt accagacacc gtttctgcta ttggcattct
    14101 gacgattacc cccagcgaag catctatcat cgcatgtgat atcgccacaa aatcaggcgc
    14161 tgtagagatc ggttttcttg atcgttttac cggtgccgta gtgctgactg gtgatgtttc
    14221 tgctattgaa tatgcactta gccaggttac ccgtacgctg ggtgaaatga tgcgctttac
    14281 cgcctgcccc attacgcgga cctgacgtta tgcaacgtat gatgcttatc ggccccagcc
    14341 agtgcggaaa aacgtcactc acgcaatgtc tgcgtggcga aaatatccat tatcagaaaa
    14401 cgcaggcaat cgtctggtcc ccgaccacca ttgatacacc aggcgaatac ctcgaaaacc
    14461 gcagcctcta cagtgcattg ttggtcagtg cctgcgaagc agacattatc gcgctggtac
    14521 ttaatgcgga cgcaccgtgg tcaccttttt cgccaggctt tacctcgtca atgaatcgcc
    14581 cggtcattgg agtagtaacc aaagcagatt tagccagcat ggaacaaata tctttggtga
    14641 aatgttggtt acgggaagct ggagcgcaca acgtgttagt aaccagtgcg gttaataaca
    14701 atggagttac agaacttttt gccttgctgc atacagaaga aggctgccgc taatttcgat
    14761 aggttctcag atgagaatga tgacggtgat acacagataa gaatcaatga gcaatgaggg
    14821 ttgccgggca accctcattg aataaaacag gaattacttc gcttcgccgt ttttcatttc
    14881 gcccatagct ttcagctttt tggagatatc gcggcgttct ttggagagct cggcattttt
    14941 gatgatgtaa tcgtcaacgc gatcttcata gtcacctttc atgctggcga tgatgccctg
    15001 gattgcttca acgctcatcc ctggcttgat gtaatcgctc aggttgtcca gcagcaggac
    15061 gcgcttctgg ttgtcacgga tctttttctc aacgtcctga atttcacgtt gcagtttgtt
    15121 cttacgacgg aacagacgaa caaattccag tacgtcctgg aatgaaggct tggtagtttc
    15181 catttttata cccctgataa tgtgagagtc ggattcgttt aagcaaccgc tattcgttag
    15241 ggccaacatt actcatagct gccgcagatg acaatgcttt tatccctttt cactatcata
    15301 ccctttatcc ttgctgaatc gaagcagcag caagatgatt ctgaagttca ggaattattt
    15361 gcgcaaggcc cggcaaatca gattcgatag caactcaagc tgatgtgccg tttccatgtt
    15421 cagccacaca taaccataga ttggtgtttc cacaaccttc aggtcatggt ggttatcgag
    15481 caactgacgc agctcttcca cagcatcgtt caatttttcc gtattggcaa aaaccggctg
    15541 cggattacct tcgtacagcg catgaacaag gctcagcagt atttgctgca tcatgtgctg
    15601 ggtatcacga agtttttgcg cgttcaataa cacgaaatgg ctggggcgcg tggcccagta
    15661 tgcattgatt tgcaactcca gcatacaaac cagattgcga ttaatggtct ggataccttc
    15721 atatatcgat tttggaatac gggtttcttt gctggcgggc gcaatcagcc cacgcatttt
    15781 cacggcatcg gtcaggagtt tttgtagatg gctttccaga cgtgggcgtt cgagtaagtt
    15841 cggtgagaat gcagattgat agacccgatt atactcggtc agacttttcg ccagttgaat
    15901 gcgccagtgg atgaacgccc gttgtggcca gataccggta aacaacattg ccagtaaaga
    15961 gccgaggatc acatcgccgc ttcgccataa cgccgtatca atttcacctg tcggggaacc
    16021 cacaacaatt gccagcgtca ccccaatcaa taaaccttga tatggtttct tgcccagcgc
    16081 cagccaaccg caaaggaaca tggccgccgc gcaccagact aacatcagcg gtaacgagat
    16141 taactccagt tgcagagcga taagacctaa aatcgacccc aacaccgtac cgccaatacg
    16201 ttcgaaggca cgagggacaa cgttccccca gaacgagatt ggccccataa tcaccaccat
    16261 ggtgaccagc ggccaggtgc tttccgggat agtaaacagg cggataatga gaaaagtgag
    16321 caggaatgcc agcgcgaccc gagtaccatg cacaatgcgg taatggcggt atacccggat
    16381 ttcaaacggg cttaatgact tatcggcacg cacgcagaaa ctccgtaatg aagattaaaa
    16441 gcgctcataa ctcatctgtc ggccagaagg cgcgcctctg aattatccga cggacaaacc
    16501 gggaacagtg taatttcccg gcaggagcga aaaaattgaa aggcgcatct tactcttttc
    16561 gctttcaaaa aaagagtggt cttcgcgtca acacaccgcc ctgagatgaa ttagtgatgt
    16621 tttggctgca ccgcaacata catttcgata tcccagtacc catcttccgc gccattgttc
    16681 aagtaaacct cgaagcaggg ctttggtaac atttcataag cactgtcctg caagaggcta
    16741 ttaaagaact gataccaggg tttagcaaaa tcatcaccga ctacacgagc caccgccacc
    16801 gcatactgac cacctgaaat ttctgtcaga atgacgccct cactgttttc ggggagcgta
    16861 aagttattcg gtaccgtcac gacggtgtcg cagcgtaatt tttcggcggg tgtttcatct
    16921 ggattgtcgt aatagacagc aacccactcc ttcggcacaa tatttttgct atctatccac
    16981 atcatcaact gctcaaaacc tttctttacc gtctgctccc acgggccaac aagatggaaa
    17041 cctgcaacgg tacgtttctc ttcctgctta atctcgtagt tcatgacgcc tccattaaca
    17101 ctgtttttat atacagtata gttgcaatat taaaaccaca aggaatgagt gttgattatg
    17161 cgagcagact cgcactcctg ccagtctgct gcaaaagaaa ggtcaggcct tatggtggaa
    17221 ataatcactc aggcgggaaa acatgccgcc ttccccgaca gattccaggg taaccagcgg
    17281 ccagtgcgcc acctgtttat cacggtcgta aagttcaatt tcccctaccc gctgatgggc
    17341 gctaattggc gcggtgagct ctttaccatc aagggtatat ttggctttga tatgtggaat
    17401 ttcggctttc ggtagcacca tccagaactc ttgttccgtt cccaggtcga tattttcttt
    17461 atcgccatac cagatgcgtt ccgttccgac ctttttccca cggtgcaaaa tttgcaccgt
    17521 agtaaagttt tgttgccccc aacgcagtaa ttttcttgcc tcttcctcac gaccttttgc
    17581 actgtcagcc cccattacca ctgcaatgag acgacgctgc ccatctacag ccgaagcaat
    17641 gagattaaac ccggcaccag aagtatgacc cgttttcagg ccgtcaacat tcatggtttt
    17701 atcccacaac aacccgttac ggttttgctg ggtgataccg ttccaggtga ggcttttctc
    17761 actgtacata tgataaaact cgggctcgcc gtggatgata gcgcgagaaa gcacagctaa
    17821 atcataagcc gagctatgct ggccaggtgc atccagacca tgcactgttt caaaatgcgt
    17881 atccttgaga tgcagcttct cggcatagtt gttcatcatt gcaacaaact gccgttgccc
    17941 accggcaata tagtcagcca gtgcaacaca agcatcattt ccggaatcca caattaaacc
    18001 acggcttaaa tcacgtaccg atacgcgatc gccctctttc aaaaacatca gtgaagaacc
    18061 gacaaacact ggattatctt tcgcccacgc atcgcgccct acggtgacaa tatcgtctgg
    18121 cgtaattcga tgactatcga tagcgcgatc cacgacataa cccgtcatca gctttgtcag
    18181 gctggcggga ttgcgctgtt gatgctcatt acccgcggtg aggatctgtc cggtggtgta
    18241 atccatcaat acccaggacc cggcatgaat ctctggaggc tgaggtgaaa aaggaatgtt
    18301 ttccgccgca aaaccagacg ataagttaaa aacgaacaaa gaagcagcaa taataagacg
    18361 gcgtttcaac agcaaaccct caggagtttc aaatagctgt tctttttacg gaaatactta
    18421 tgaactggct ggaataaagt gcaagaaaat gtgactaacc tctcattttt atctgacatg
    18481 atctgttgcc actcgctgcc aaattgtggc gctaaagctg attagcacgg tgatatttga
    18541 tactctggca gacagcagag ataacggatt taacctaatg atgaatgacg gtaagcaaca
    18601 atctaccttt ttgtttcacg attacgaaac ctttggcacg caccccgcgt tagatcgccc
    18661 tgcacagttc gcagccattc gcaccgatag caaattcaat gtcatcggcg aacccgaagt
    18721 cttttactgc aagcccgcgg atgactattt accccagcct ggagcagtat taattaccgg
    18781 tattaccccg caggaggcac gggcgaaagg agaaaacgaa gccgcgtttg ccgcccgtat
    18841 tcactcgctt tttaccgtac cgaagacctg tattctgggc tacaacaatg tgcgtttcga
    18901 cgacgaagtc acacgcaacg ttttttatcg taatttctac gatccttacg cctggagctg
    18961 gcagcatgat aactcgcgct gggatttact ggatgttatg cgtgcctgtt atgccctgcg
    19021 cccggaagga ataaactggc ctgaaaatga tgacggtcta ccgagctttc gccttgagca
    19081 tttaaccaaa gcgaatggta ttgaacatag caacgcccac gatgcgatgg ctgatgtgta
    19141 cgccactatt gcgatggcga aactggtaaa aacgcgtcag ccacgcctgt ttgattatct
    19201 ctttacccat cgtaataaac acaaactgat ggcgttgatt gatgttccgc agatgaaacc
    19261 cttggttcac gtttccggaa tgtttggggc atggcgcggc aataccagct gggtggcacc
    19321 gctggcgtgg catcctgaaa atcgcaatgc cgtaattatg gtggatttgg caggagacat
    19381 ttcgccatta ctggagctgg atagcgacac attgcgcgag cgtttatata ccgcaaaagc
    19441 cgatcttggc gataacgccg ccgttccggt taagctggtg catatcaata aatgtccggt
    19501 actggcccag gcgaatacgc tacgcccgga agatgccgac cgactgggaa ttaatcgtca
    19561 gcattgcctc gataacctaa aaattctgcg tgaaaatccg caagtgcgtg aaaaagtggt
    19621 ggcgatattc gcggaagccg aaccgtttac gccttcagat aacgtggatg cacagcttta
    19681 taatggcttt ttcagtgacg ccgatcgtgc agcaatgaaa attgtgctgg aaaccgagcc
    19741 gcgtaattta ccggcactgg atatcacttt tgttgataaa cgaattgaaa aactgttgtt
    19801 caattatcga gcgcgcaact tcccggggac gctggattat gccgagcagc aacgctggct
    19861 ggagcaccgc cgccaggtct tcacgccaga gtttttgcag ggctacgctg atgaattgca
    19921 gatgctggca caacagtatg ctgatgacaa agagaaagtg gcgctgttaa aagcgctttg
    19981 gcagtacgcg gaagagatcg tctgaaacaa aaaagccgga gcgacaaact ccggcttttt
    20041 gagatttacc ggatgattat gtcatccggt ttgtaagatt acgctacgtc ttcgtactgc
    20101 ggaaccgggt tacggaaact cttggttacg caagccaggt aaatcagacc gatagcagcc
    20161 cagatcagac ccagaaccat tgagctttct tccaggttaa cccacagcgc accaacggtc
    20221 agcgcaccac acatcggcag gaacagatac tggaagtgat ctttcagcgt cttgttacgc
    20281 ttctcacgga tccagaactg cgagatgacc gacaggttaa cgaaggtgaa cgccaccaac
    20341 gcaccaaagt taatcagcgc cgtagccatt accaggtcga agttgattgc cagcagggca
    20401 atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg tacataaccg
    20461 aagaagcttt tcgggaacac gccgtcacga cccattacgt acatcagacg cgctacgcct
    20521 gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg agaagatcag cgcgccaacc
    20581 tggaacgctt tacccgcaac gtacagcatg atttcaggct gtgacgcatc cggatcttta
    20641 aagcgagaga tatccgggaa gtacagctgc aggaagtaag ttgcaaagat gaagatcatg
    20701 ccgccgatca gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc atctttggtt
    20761 tcttccgaca ggttgctgat gccgtcaaaa ccggtaaagg agaagcacag gattgtcgcc
    20821 ccggtaatca tcgggataac atgcgcatcg ccagaccaga acggacgagt gctcgccagc
    20881 gtaccagcgc cttcaccttc aaatacgcca taaacaacca tgcccagaat caccgcgatc
    20941 agcactacct gcaacacgac gattacagtg ttgaagttcg ctacggattt cagactacgc
    21001 aggttaaagg cggtcataaa ggccaccagc gccaccacaa acatccacga tggaatggaa
    21061 ggcaccagag cttcaaaata gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga
    21121 tagtcgagca gagaagacca acccaccata aagccgacag tcgggctaat ggatttctgg
    21181 gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa ccagcttccc gtagctcaga
    21241 gccgtaaaca ggatcgcaat caatgcgaac gcataggctg tcggcacatg accatccgtg
    21301 aggcctgaaa cgataccaaa agtatcaaac agcgtcatcg gctgcatata ggctagaccc
    21361 atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga
    21421 gtaacgttat gcgacatggt cattctcctt tacggtgata accgtcgcgt aagcaaaaaa
    21481 ttgccccatt tttttggatt cctcagcgac aacaactgtc gatttttagt aaatatctat
    21541 ccggtacgaa gcccggcctc ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg
    21601 ctaattaaac gtcggttttt agtcattttt tgacagcggc gcattgtgcc taaatggggg
    21661 ggaaatgaca agagaatgag aggcttgtca taataatttt tctttaaatg gctgattttc
    21721 cgtcatcaga ttttgcataa acaccggcga tcgcactatt tgctaaaatt tcgtcccgcc
    21781 accaaccact ggcaagccct gcggtctgtt cattccagcc aatccagacg ggttcaaaga
    21841 gagtttgggc gaccactttc ttctcgatta acgcgccact atcgagaaaa cgttgcgcca
    21901 gataccgtgg taaatagccg cagccaaggc cgctaatttg cagctccagc ttggttttaa
    21961 aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg tgaggccgta gaagcgcctg
    22021 cctgcgcagt gtcccccacc acaatcgccc gatatcgttt aatgatacga cggtttaacg
    22081 gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc aaagacttgt tcaagatcgc
    22141 ccaaccgcga gaaaccaaac tcactggaag atggtggctc atgcatcgcg ccaacaataa
    22201 tatccgctcg cccctgagtc agtgcatccc aggaaccacc gagtacgcca ttgataaact
    22261 tcaggcgcgt cacgctatga tgttgataaa aagcctcaat gagaggcgca agcagagaaa
    22321 aaggaaacgt gtcgtccacg ccaatcacca gctcattttc ccagccttca tgcagtttga
    22381 tagcctgctt ttccagttct cgcacggtat gcagaacttc ccgccctttc tctaataaca
    22441 tttttccggt gcgggtgaat ttagcgcggt gaccgctacg atcaagcaat tgaatattaa
    22501 ggtcgctttc cagcttgtga acggtataac tcagagcgga cggcgtcttg tagagtttag
    22561 ccgacgccgc cgcaaaactg ccttcttttt ctaaggcatc gaggatcatc aacacatcca
    22621 gcagtggttt catacacgcc cccttgcagt gtatgcggcg tcctgctggc gtgatcgttt
    22681 actccagcgg cattaccagc ggatcgggat actggtattc aaatcccagt tcattacaaa
    22741 tccgactgcc atcaataatc ttacctttgc cgctgtccag actatttctg aactgcggag
    22801 gttccagccc cagtaaacgg gccatctgcg gatagaaaac attacgcgca gggtgagctg
    22861 gcgcacatat attatagatg tgtccgcctt taggtgcctg taacaacaga gtgatagcac
    22921 caataacatc ttctaaatgg actaaattaa caccatgttc accatcaggg gcggtttttc
    22981 cggcaaagaa gcgtccggga tgacgtcccg gtccgaccag ccccgcaaga cgcagaatat
    23041 cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag ttcttctaat actcgtccac
    23101 tgttggtcac cggattacgc ggggtggttt ctttcaccgt gccttgcgcg tcgccataga
    23161 cagatgttga gctggtaaaa ataattcggg gaatacgatg ggccagcgcg ctatccacta
    23221 actcttgtac cgcttgtaaa tagaactcat cgccggggcc gctacgacgt gccggaagcg
    23281 taatgaccag cgcgtcggca tccatcaggg catccagatc gtcagaatcg caaactaact
    23341 caggctccat gcgaagcaga tagctatcaa tgccactcat tcgggccgct tcgacaccat
    23401 cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg
    23461 gcatgcctaa ccaccctaac ccgacaattg cgaccttttt catccgttat ctcctgatac
    23521 accttagatc tataaggcta cgctagcgta tcaaaactga caattcattc tatgaatgaa
    23581 tctgttcaat aatgataacg acatgctgca atgagtagtg aatcgtcggt aaccgcgtat
    23641 accagacggt gctcctctgt aatgcgtcgg gaccagaaac ctgacaaatt atgtttcagg
    23701 ggttctggtt tccccttacc ttcaaatggt gttctgcggg tatctttgat aagttcattg
    23761 atctttttaa caattcgctt atctgtttcc tgccagtaca gataatcatc ccatgattcc
    23821 tcagaccaga ttagtttcac tcaatgatgt ccttttccgt tcctttgcca gatttcaggc
    23881 tatcgattga gtccatcaat ctccgggcgt tagcggggga gcgcagtaga taagccgtct
    23941 cctccaggga gttgtattct tcgagtgaca tcagaacaca agcctctcca ttctgacgag
    24001 taataaggat cggggcatga tcttcaacgg ctttcatcat tgttgccgac aaattctgac
    24061 gcgcttcgct gtagctaatt gtacgcatgt caatctcctc ttttgtacag ttcattgtac
    24121 aatgatgggc gttaattaac tatttattaa ttagtttgta gatcaaggta ttgtcagtga
    24181 gacgaaaatc caggcttcgc tatttttggt gccgtcagct tagaggacag tcctcttagc
    24241 cccctccttt ccccgctcat tcattaaaca aatccattgc cataaaatat ataaaaaagc
    24301 ccttgctttc taacgtgaaa gtggtttagg ttaaaagaca tcagttgaat aaacattcac
    24361 agagactttt atgacacgcg ttcaatttaa acaccaccat catcaccatc atcctgacta
    24421 gtctttcagg cgatgtgtgc tggaagacat tcagatcttc cagtggtgca tgaacgcatg
    24481 agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgcggttg ataacggttc
    24541 agacaggttt aaagaggaat aagaaaatga cagacaacac tcgtttacgc atagctatgc
    24601 agaaatccgg ccgtttaagt gatgactcac gcgaattgct ggcgcgctgt ggcattaaaa
    24661 tcaatcttca cacccagcgc ctgatcgcga tggcagaaaa catgccgatt gatattctgc
    24721 gcgtgcgtga cgacgacatt cccggtctgg taatggacgg cgtggtagac cttgggatta
    24781 tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg agcccagggt gaagatccgc
    24841 gctactttac cctgcgtcgt ctggatttcg gcggctgccg cctttcgctg gcaacgccgg
    24901 ttgatgaagc ctgggatggc ccgctctcct taaacggtaa acgtatcgcc acctcttatc
    24961 ctcacctgct caagcgttat ctcgaccaga aaggcatctc ttttaaatcc tgcttactga
    25021 acggttctgt tgaagtcgcc ccgcgtgccg gactggcgga tgcgatttgc gatctggttt
    25081 ccaccggtgc cacgctggaa gcaaacggcc tgcgcgaagt cgaagttatc tatcgctcga
    25141 aagcttgcct gatacaacgc gatggcgaaa tggaagaatc caaacagcag ctgatcgaca
    25201 aactgctgac ccgtattcag ggcgtgatcc aggcgcgcga atcaaaatac atcatgatgc
    25261 acgcaccgac cgaacgcctg gatgaagtca tcgccctgct gccaggtgcc gaacgcccaa
    25321 ctattctgcc gctggcaggt gatcaacagc gcgtagcgat gcacatggtg agtagcgaaa
    25381 ccctgttctg ggaaacgatg gaaaaactga aagcgctggg tgccagttca atcctggtcc
    25441 tgccgattga aaaaatgatg gagtgatcgc catgagcttt aacacaatca ttgactggaa
    25501 tagctgtact gcggagcaac aacgccagct gttaatgcgc ccggcgatct ccgcttctga
    25561 aagcattacc cgcaccgtta acgatatcct cgataacgtg aaagcacgcg gcgatgaggc
    25621 cctgcgtgaa tacagcgcga aatttgataa aaccacggtt accgcgctga aggtgtctgc
    25681 tgaggagatc gccgccgcca gcgaacgcct gagcgacgag ctaaaacagg cgatggcggt
    25741 ggcagtaaag aatattgaaa ccttccacac tgcgcaaaaa ctgccgccgg tagatgtcga
    25801 aacgcagcca ggcgtgcgtt gccagcaagt cacgcgcccg gtcgcttcag ttgggttgta
    25861 tattcctggc ggctccgccc cgctcttctc aacggtatta atgctggcaa ctccggcgcg
    25921 tattgcgggc tgtaaaaaag tggtgctgtg ctcaccgccg ccgattgccg acgaaattct
    25981 ttacgcggcg cagctgtgcg gtgtgcagga tgtgtttaac gtcggcggcg cacaggccat
    26041 tgccgcactg gcgtttggta cggaatctgt gccgaaagtt gacaaaatct tcgggccagg
    26101 taacgccttt gtcaccgagg caaaacgtca ggtgagccag cgtctggacg gtgcggcgat
    26161 cgatatgccc gcaggcccgt cggaagtgct ggtgattgct gacagcggcg ctacgccgga
    26221 tttcgtggct tctgatttgc tttctcaggc tgaacacggc ccggactcgc aggtgatttt
    26281 actgacgccc gatgctgata tggcgcgtcg cgttgccgag gctgtcgaac gccaactggc
    26341 agaactgccg cgtgccgaaa ccgcccgcca ggcactgaac gccagccgcc tgatcgtgac
    26401 taaagattta gcgcagtgcg tagagatctc caaccagtac ggcccggagc acctgatcat
    26461 ccagacccgc aacgcccgcg atctggtcga tggcatcacc agcgccggtt cggtatttct
    26521 tggtgactgg tcacccgaat cggcgggtga ttacgcctcc ggcaccaacc acgtactgcc
    26581 gacttacggt tacaccgcca cctgttccag cctcgggctg gcagatttcc agaagcgtat
    26641 gaccgtgcag gaactgtcga aagaaggttt ctccgcgctg gcttcgacca tagaaacact
    26701 ggccgccgcc gagcgcctga ccgcccataa aaatgccgtt actttgcgtg ttaacgccct
    26761 taaggagcaa gcatgagcac cgtgactatt accgatttag cgcgtgaaaa cgtccgcaac
    26821 ctgacgccgt atcagtcggc gcgtcgtctg ggcggtaacg gcgacgtctg gctgaacgcc
    26881 aacgaatacc ccacagccgt ggagtttcag ctcactcaac aaacgctcaa ccgctatccg
    26941 gaatgtcagc caaaagcggt gattgaaaat tacgcgcaat atgcaggcgt aaaaccggaa
    27001 caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac tgctgattcg cgctttttgc
    27061 gaaccgggta aagacgccat cctctactgc ccgccaacgt acggcatgta cagcgtcagc
    27121 gctgaaacca ttggcgtcga gtgccgcaca gtgccgacgc tggacaactg gcaactggac
    27181 ttacagggca tttccgacaa gctggacggc gtaaaagtgg tctatgtttg cagccccaac
    27241 aacccgaccg ggcaactgat caacccgcag gattttcgca ctctgctgga gttaacccgc
    27301 ggtaaagcga ttgtggttgc cgatgaagcc tatatcgagt tttgcccgca ggcatcgctg
    27361 gctggctggc tggcggaata tccgcacctg gctattttgc gcacactgtc gaaagctttt
    27421 gctctggcgg gccttcgttg cggatttacg ctggcaaacg aagaagtcat caaccttctg
    27481 atgaaagtga tcgcccccta cccgctctcg acgccggtcg ccgacattgc ggcccaggcg
    27541 ttaagcccgc aggggatcgt cgccatgcgc gaacgggtgg cgcaaattat tgcagaacgc
    27601 gaatatctga ttgccgcatt gaaagaaatc tcctgcgtgg agcaggtttt tgactctgaa
    27661 accaactaca ttctggcgcg ctttaaagcc tccagcgcag tgtttaaatc tttgtgggat
    27721 cagggcatta tcttacgtga tcagaataaa caaccctctt taagcggctg cctgcgaatt
    27781 accgtcggaa cccgtgaaga aagccagcgc gtcattgacg ccttacgtgc ggagcaagtt
    27841 tgatgagtca gaagtatctt tttatcgatc gcgatggaac cctgattagc gaaccgccga
    27901 gtgattttca ggtggaccgt tttgacaaac tcgcctttga accgggcgtg atcccggagc
    27961 tgctgaagct gcaaaaagcg ggatataagc tggtgatgat caccaatcag gatggtctgg
    28021 gaacacaaag tttcccgcag gcggattttg atggcccgca caacctgatg atgcagatct
    28081 tcacctcgca aggcgtgcag tttgatgaag tgctgatttg cccgcacctg cccgccgatg
    28141 agtgcgactg ccgtaaaccg aaagtaaaac tggtggagcg ttatctcgct gagcaagcga
    28201 tggatctcgc caacagttat gtgattggcg atcgcgcgac cgatattcaa ctggcggaaa
    28261 acatgggtat taatggttta cgctacgacc gcgaaaccct gaactggccg atgattggcg
    28321 agcaactcac taaacgagac cgttacgccc atgtagtgcg caacaccaaa gagacgcaga
    28381 ttgacgttca ggtatggctg gatcgcgaag gtggcagcaa gattaacacc ggcgttggct
    28441 tctttgatca tatgctggat cagatcgcca ctcacggcgg tttccgcatg gaaatcaacg
    28501 tcaaaggcga cctctatatc gacgatcacc acaccgtcga agataccggc ctggcgctgg
    28561 gtgaagcgtt aaaaattgcc ctcggcgata aacgcggtat ttgccgcttt ggttttgtgc
    28621 tgccgatgga cgaatgcctt gcccgctgtg cactggatat ctctggtcgc ccacacctgg
    28681 aatataaagc tgaatttacc taccagcgcg tcggcgatct cagcaccgag atgatcgagc
    28741 acttcttccg ttcgctctct tacaccatgg gcgtgacgtt gcacctgaaa accaaaggta
    28801 aaaacgatca tcaccgtgta gagagtctct ttaaagcctt tggtcgcacc ctgcgccagg
    28861 ccatccgcgt ggaaggcgac accctgccct cgtcgaaagg agtgctgtaa tgaacgtggt
    28921 gatccttgat accggctgcg ccaatttgaa ctcggtgaag tcagccattg cgcgtcacgg
    28981 ttatgaaccc aaagtcagcc gtgacccgga cgtcgtgttg ctggccgata aactattttt
    29041 acccggcgta ggcaccgcac aagcagcgat ggatcaggtg cgtgagcgcg agctgtttga
    29101 tctcatcaaa gcctgtaccc aaccggtgct gggcatctgc ttagggatgc aactgctggc
    29161 gcggcgcagc gaagagagca acggcgtcga tttgctgggc attatcgacg aagacgtgcc
    29221 gaaaatgacc gactttggtc tgcccctgcc gcatatgggc tggaaccgcg tttacccgca
    29281 ggcaggcaac cgcctgtttc aggggattga agacggcgcg tacttttact ttgttcacag
    29341 ctacgcaatg ccagtcaatc cgtggaccat cgcccagtgt aattacggcg aaccgttcac
    29401 cgcggcggta caaaaagata acttctacgg cgtgcagttc cacccggagc gttctggtgc
    29461 cgctggcgct aagctgctga aaaacttcct ggagatgtga tgattattcc ggcattagat
    29521 ttaatcgacg gcaccgtggt gcgtctccat cagggagatt atggcaaaca acgcgattac
    29581 ggtaacgacc cgctgccacg cttacaggat tacgccgcgc agggtgccga agtgttgcat
    29641 ctggtggatc tgaccggggc aaaagatccg gctagacgtc aaatcccgct gattaaaacc
    29701 ctggtggcgg acgttaacgt tccggtgcag gttggcggcg gcgtgcgtag cgaagaagac
    29761 gtggcggcgt tactggaagc gggcgttgcg cgcgtggtgg ttggctccac cgcggtgaaa
    29821 tcaccagaaa tggtgaaagg ctggtttgaa cgtttcggtg ccgatgcctt agtgctggcg
    29881 ctggatgtcc gtattgacga gcaaggcaac aagcaggtgg cggtgagcgg ctggcaagag
    29941 aactcgggcg tttcactgga acaactggtg gaaacctatc ttcccgtcgg cctgaaacat
    30001 gtgctatgta ccgatatctc gcgcgacggc acgctggcag gttctaacgt ctctttatat
    30061 gaagaagtgt gcgccagata cccgcaggtg gcatttcagt cctccggcgg cattggcgac
    30121 attaacgatg ttgctgccct gcgaggtact ggcgtgcgcg gcgtaatagt tggtcgggca
    30181 ttactggaag gtaaattcac cgtgaaggag gctatcgcat gctggcaaaa cgcataattc
    30241 catgtctcga cgttcgtgat ggtcaggtgg tgaaaggcgt acagtttcgc aaccatgaaa
    30301 tcattggcga tatcgtgccg ctggcaaaac gttacgccga agaaggtgca gacgaactgg
    30361 tgttctacga tatcaccgct tccagcgatg gccgtgtggt agataaaagc tgggtatctc
    30421 gcgtggcgga agtaatcgac attccgtttt gtgtggcggg tgggattaag tctctggaag
    30481 atgccgcgaa aattctttcc tttggcgcgg ataaaatctc cattaactcc cctgcactgg
    30541 cagacccgac gttaattact cgcctggcgg atcgctttgg cgtgcagtgt attgtggtcg
    30601 gcattgatac ctggtacgac gccgaaaccg gtaaatatca tgtgaatcaa tataccggcg
    30661 atgaaagccg cacccgcgtc actcagtggg aaacactcga ctgggtgcag gaagtgcaaa
    30721 aacgcggtgc cggagaaatc gtcctcaaca tgatgaatca ggacggcgtg cgtaacggtt
    30781 acgacctcga acagctgaaa aaagtgcgtg aagtttgcca cgtcccgctg attgcctccg
    30841 gtggcgcggg caccatggaa cacttcctgg aagccttccg cgatgccgac gttgacggtg
    30901 cgctggctgc ttccgtattc cacaaacaaa taatcaatat tggtgaatta aaagcgtacc
    30961 tggcaacaca gggcgtggag atcaggatat gttaacagaa caacaacgtc gcgaactgga
    31021 ctgggaaaaa accgacggac tgatgccggt gattgtgcaa catgcggtat ccggcgaagt
    31081 gctaatgctg ggctatatga acccggaagc tctggacaaa accctcgaaa gcggcaaagt
    31141 caccttcttc tcgcgcacta aacagcgact gtggaccaaa ggcgaaacgt cgggcaattt
    31201 tctcaatgtg gtgagtattg ccccggactg cgacaacgac acgttactgg tgctggcgaa
    31261 tcccatcggg ccaacctgcc acaaaggcac cagcagctgc ttcggcgaca ccgctcacca
    31321 gtggctgttc ctgtatcaac tggaacaact gctcgccgag cgcaaatctg ccgatccgga
    31381 aacctcctac accgccaaac tgtatgccag cggcaccaaa cgcattgcgc agaaagtggg
    31441 cgaagaaggc gtggaaaccg cactggcagc aacggtacat gaccgctttg aactgaccaa
    31501 cgaggcatct gatttgatgt atcacctgct ggtgctgttg caggatcagg atctggattt
    31561 aacgacggta attgagaacc tgcgtaaacg gcatcagtga gttgcgggtg atcagatgcg
    31621 atgttgttgt cgcatccggc aaaaaaaacg ggcaaggtgt caccaccctg cccgttttct
    31681 ttaaaaccaa aagattactt cgcgttgtaa ttacgcagag cgttacgccc cagcacaatc
    31741 cccgcgccaa ccatgccgcc cagcagcact gccagaatca aggtaattgc ctttttcggg
    31801 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
    31861 tcatcaacct ttaagttttc aatatccaac aggttttgac gagtctgata gtagtttggc
    31921 gagaacacca acggacgagt cgcctcatgc ttaatcatcg actccagcgc ttcgctcccc
    31981 aaaaggaaca acgtatcttg tgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
    32041 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
    32101 tcttgcgcga ctacttcctg agttcttaaa gagtcctgca agtttttcat acgcagcgcg
    32161 atgttgtctt tcaggtcttt ttccagttcc tcattaacct ggtcgtcaac ttgctggata
    32221 tacttcgcca gctgcttctg agcaccttca ggtgtttcac caacataaga caccgctaaa
    32281 ggtaatgact gatttttaac ggtaggctca atggtcagct tttctgcttc ttcctgatta
    32341 tccagcgttt ccgctaatgc tgaaaatgca gtgctgtaac gtccgataag tgaagcctga
    32401 atgtcggaga ctttcggtac agccgatccg tagatcacat tcatcgcgtt ggtataacca
    32461 gcgatttgcc ccacatcagg ttgagtaacg atagctgtgg aagtccactt ttctttggca
    32521 acggccagat accctatagc cagggcaata gcaacaatga cagaaatgat gatagtcatc
    32581 ttgccgcgcc acaactgcac cagtaaatca atcaaatcaa tctgttccgg gtcatggttt
    32641 tgcccagaaa cattattatt ttctactctc atcattaccc taacggacga aataatttta
    32701 agtcgccata gtttagcgat aatcgccatg aatgctatag gataaatgat aaaaagaatg
    32761 aattattaca aagggaacat gagagaatca gggcgaaaat cgccctgata acaggatgtt
    32821 agtcactgcc aaagagatcg cgggtgtaga ctttgtctgc cacatcctta agctcttctg
    32881 ccatacggtt ggaaataata acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
    32941 gtcgagagtt gaagaatgaa tcttctttca tcaccggctc gtagatgatc acttcaacac
    33001 cttttgcctt gatacgtttc ataatcccct gaatggaaga cgcgcggaag ttatctgacc
    33061 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcgtgac aaaatggcat
    33121 cggcaataaa atctttacgc gtgcggtttg catcgacaat tgccgagatc aggttattcg
    33181 gcacagactg gtagttcgcc agtaactgct tggtatcttt cggtagacaa taaccaccat
    33241 agccaaacga cggattgttg taatggttgc caatacgcgg atcgagacaa acaccttcga
    33301 taatctgacg agtattcaga cctaaacttt ctgcatagct atccagctcg ttaaagtatg
    33361 ctacgcgcat cgccagatag gtattagcaa acagtttaat cgcttctgct tcagtggagt
    33421 cggtaaacag ggtcgggata ttttgcttaa tcgcgccttc ctgtaataac gccgcaaaac
    33481 gttctgcgcg ttctgaacgc tcaccgatga caatacgtga cggatgaagg ttatcgtaaa
    33541 gagctttacc ttcacggaga aattccggag agaaaataat attttcagta cgatatttct
    33601 tatgcatcgc ttcggtaaaa ccaacgggaa ccgttgattt gatgaccata accgcataag
    33661 gatttatctc aactacgtct ttaattactg attctacact cgatgtattg aaataattag
    33721 ttttaggatc atagtcggtt ggagtggcga tgat
//
LOCUS       DAZKJR010000039        33605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKJR010000039 DAZKJR010000000
VERSION     DAZKJR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.039"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..330
                     /gene="ytcA"
                     /locus_tag="ACHOWS_001916"
     CDS             55..330
                     /gene="ytcA"
                     /locus_tag="ACHOWS_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFQ0047492.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            349..1380
                     /gene="mdtN"
                     /locus_tag="ACHOWS_001917"
     CDS             349..1380
                     /gene="mdtN"
                     /locus_tag="ACHOWS_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFQ0047493.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGIDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1380..3431
                     /gene="mdtO"
                     /locus_tag="ACHOWS_001918"
     CDS             1380..3431
                     /gene="mdtO"
                     /locus_tag="ACHOWS_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFQ0047494.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            3428..4894
                     /gene="mdtP"
                     /locus_tag="ACHOWS_001919"
     CDS             3428..4894
                     /gene="mdtP"
                     /locus_tag="ACHOWS_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFQ0047495.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPFYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTLRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            5092..7239
                     /gene="fdhF"
                     /locus_tag="ACHOWS_001920"
     CDS             5092..7239
                     /gene="fdhF"
                     /locus_tag="ACHOWS_001920"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5509..5511,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFQ0047496.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNKTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDTDQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7333..8022
                     /gene="yjcO"
                     /locus_tag="ACHOWS_001921"
     CDS             7333..8022
                     /gene="yjcO"
                     /locus_tag="ACHOWS_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFQ0047497.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(8440..9753)
                     /gene="gltP"
                     /locus_tag="ACHOWS_001922"
     CDS             complement(8440..9753)
                     /gene="gltP"
                     /locus_tag="ACHOWS_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFQ0047498.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(10095..10691)
                     /gene="nrfG"
                     /locus_tag="ACHOWS_001923"
     CDS             complement(10095..10691)
                     /gene="nrfG"
                     /locus_tag="ACHOWS_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFQ0047499.1"
                     /translation="MKQSQIPVKMLTTLTILMAFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10688..11071)
                     /gene="nrfF"
                     /locus_tag="ACHOWS_001924"
     CDS             complement(10688..11071)
                     /gene="nrfF"
                     /locus_tag="ACHOWS_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFQ0047500.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEAEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(11064..12782)
                     /gene="nrfE"
                     /locus_tag="ACHOWS_001925"
     CDS             complement(11064..12782)
                     /gene="nrfE"
                     /locus_tag="ACHOWS_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFQ0047501.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(12802..13758)
                     /gene="nrfD"
                     /locus_tag="ACHOWS_001926"
     CDS             complement(12802..13758)
                     /gene="nrfD"
                     /locus_tag="ACHOWS_001926"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFQ0047502.1"
                     /translation="MMQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTKPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13755..14426)
                     /gene="nrfC"
                     /locus_tag="ACHOWS_001927"
     CDS             complement(13755..14426)
                     /gene="nrfC"
                     /locus_tag="ACHOWS_001927"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFQ0047503.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(14423..14989)
                     /gene="nrfB"
                     /locus_tag="ACHOWS_001928"
     CDS             complement(14423..14989)
                     /gene="nrfB"
                     /locus_tag="ACHOWS_001928"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFQ0047504.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(15034..16470)
                     /gene="nrfA"
                     /locus_tag="ACHOWS_001929"
     CDS             complement(15034..16470)
                     /gene="nrfA"
                     /locus_tag="ACHOWS_001929"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFQ0047505.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16863..18821
                     /gene="acs"
                     /locus_tag="ACHOWS_001930"
     CDS             16863..18821
                     /gene="acs"
                     /locus_tag="ACHOWS_001930"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFQ0047506.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            19203..19517
                     /gene="yjcH"
                     /locus_tag="ACHOWS_001931"
     CDS             19203..19517
                     /gene="yjcH"
                     /locus_tag="ACHOWS_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFQ0047507.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            19514..21163
                     /gene="actP"
                     /locus_tag="ACHOWS_001932"
     CDS             19514..21163
                     /gene="actP"
                     /locus_tag="ACHOWS_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFQ0047508.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(21246..22895)
                     /gene="yjcE"
                     /locus_tag="ACHOWS_001933"
     CDS             complement(21246..22895)
                     /gene="yjcE"
                     /locus_tag="ACHOWS_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFQ0047509.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23046..24395)
                     /gene="ghxP"
                     /locus_tag="ACHOWS_001934"
                     /gene_synonym="yjcD"
     CDS             complement(23046..24395)
                     /gene="ghxP"
                     /locus_tag="ACHOWS_001934"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFQ0047510.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(24941..25405)
                     /gene="soxR"
                     /locus_tag="ACHOWS_001935"
     CDS             complement(24941..25405)
                     /gene="soxR"
                     /locus_tag="ACHOWS_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFQ0047511.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            25491..25814
                     /gene="soxS"
                     /locus_tag="ACHOWS_001936"
     CDS             25491..25814
                     /gene="soxS"
                     /locus_tag="ACHOWS_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFQ0047512.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(25817..27403)
                     /gene="pdeC"
                     /locus_tag="ACHOWS_001937"
     CDS             complement(25817..27403)
                     /gene="pdeC"
                     /locus_tag="ACHOWS_001937"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFQ0047513.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGVYLATHVDRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            27833..28114
                     /gene="yjcB"
                     /locus_tag="ACHOWS_001938"
     CDS             27833..28114
                     /gene="yjcB"
                     /locus_tag="ACHOWS_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFQ0047514.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(28214..28750)
                     /gene="ssb1"
                     /locus_tag="ACHOWS_001939"
     CDS             complement(28214..28750)
                     /gene="ssb1"
                     /locus_tag="ACHOWS_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFQ0047515.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            29005..31827
                     /gene="uvrA"
                     /locus_tag="ACHOWS_001940"
     CDS             29005..31827
                     /gene="uvrA"
                     /locus_tag="ACHOWS_001940"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0047516.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(31862..32218)
                     /gene="yjbR"
                     /locus_tag="ACHOWS_001941"
     CDS             complement(31862..32218)
                     /gene="yjbR"
                     /locus_tag="ACHOWS_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0047517.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(32222..32638)
                     /gene="yjbQ"
                     /locus_tag="ACHOWS_001942"
     CDS             complement(32222..32638)
                     /gene="yjbQ"
                     /locus_tag="ACHOWS_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFQ0047518.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(32749..33462)
                     /gene="aphA"
                     /locus_tag="ACHOWS_001943"
     CDS             complement(32749..33462)
                     /gene="aphA"
                     /locus_tag="ACHOWS_001943"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFQ0047519.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         8187 a         9117 c         8713 g         7588 t
ORIGIN      
        1 tggatattga tattcactac agatattgtt cgcagcagac aggatgtatt taatatgcca
       61 acagttctct ctcgcatggc gatgcaactc aaaaaaacag cctggataat tcccgtcttc
      121 atggtttcgg gatgctcatt atctccggca atcccggtga tcggcgctta ttatcccggc
      181 tggtttttct gcgccattgc cagccttatt ttgacgctca tcacgaggcg aattattcag
      241 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
      301 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      361 ccgaaaaaag ctcctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      421 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      481 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      541 accgacaacc aggcggtgaa acagggtgat ttgctattcc gcatcgaccc acgcccatac
      601 gaagccaatc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      661 acccagcgta gcgttgacgc gcaacagttt ggtgccgact cggttaatgc cacggtagaa
      721 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      781 aaagaaggtt ttgtctcagc ggaagacgtt gaccgtgcaa gaactgcgca gcgcgccgca
      841 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      901 atcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaactg
      961 catctggaaa tggcgaccgt tcgcgcgccg tttgatggcc gggtcatttc cctcaaaacc
     1021 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctaatcga cactcgtcac
     1081 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
     1141 gcaaccattc gcctgatgag cgacagcggc aaaaccttcg agggtaaagt ggattcgatt
     1201 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc gaaagtgtct
     1261 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1321 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1381 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1441 agttaagcga gcggcggcca ggtcgcgtgc cgcagaccgt gcaactctgg gtaggctgcc
     1501 tgctggtaat tctgatctcg atgacctttg agatcccttt tgtggcgtta tcgctggcag
     1561 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtcgcg atcttgtttg
     1621 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1681 aaccgttgat ccgattgatc atcgccggac cgatcctgat gggctgtatg tttttgatgc
     1741 gcacccatcg gttggggctg gtctttttcg ccgtcgccat agtcgctatt tacgggcaaa
     1801 ccttccccgc catgctcgac tatccggaag tggtcgtgcg cttaaccctg tggtgtatcg
     1861 ttgtcggcct ctatccaacc ttgctgatga cgttaatcgg cgtgctgtgg tttcccagcc
     1921 gcgctatcac gcagatgcat caggcgctta atgatcggct tgatgatgcc attagccacc
     1981 tgacggacag cctcgcaccg ctacccgaaa cgcggattga aagagaggcg ctggcgctgc
     2041 aaaaactcaa tgtcttttgc ctcgcggacg atgccaactg gcgaactcag agcgcatggt
     2101 ggcaaagctg cgtggcaacg gtaacctaca tttactcgac gctgaatcgc tacgatccca
     2161 cctcttttgc tgattctcag gcaattattg aatttcggca aaaattagcc tcagaaatca
     2221 acaagctgca gcatgccatt gccgaaggtc agtgctggca aagcgactgg cggatcactg
     2281 aaagtgaagc gatggcggca cgggaatgta acctggagaa tatctgccag acgttattgc
     2341 aactgggtca gatggacccg aatacgccgc caacgcccgc caccaaaccg ccatcaatgg
     2401 tcgccgacgc ttttaccaat ccagactata tgcgctacgc ggtaaaaacg ctgctcgcct
     2461 gtttgatctg ttacaccttc tacagcggcg tggactggga aggcattcac acctgtatgc
     2521 tgacctgcgt gatcgtcgct aatcccaatg tcggttcgtc gtaccagaag atggtgctgc
     2581 gttttggcgg ggccttttgc ggcgcgattc tggcgctgtt attcacgtta ttggtcatgc
     2641 cctggctgga caatattgtc gaattgctgt ttgtgctggc accgattttc ctgttgggcg
     2701 catggattgc caccagctct gaacgctctt cttatatcgg cacacagatg gtggtcacct
     2761 tcgcgctcgc cacgctcgaa aacgtttttg gcccggtgta cgacctggtg gaaattcgcg
     2821 atcgcgctct gggtatcatc attggtaccg tggtgtcggc ggtgatttac acctttgtct
     2881 ggcctgaaag cgaagcgcgc acgctgccgc aaaaactggc tggcgcgctg ggtatgttga
     2941 gtaaagtaat gcggatccca cgccagcagg aagtcacagc tctgcgcact tatctgcaaa
     3001 ttcgcatagg tctgcatgcg gcgtttaatg cctgtgaaga gatgtgccaa cgcgtggcgc
     3061 tggagcgtca actggacagc gaagaacgcg ctttactgat tgaacgttcg caaacggtta
     3121 ttcgtcaggg ccgcgatatt cttcacgcct gggatgcaac ctggaactcg gcgcaggcgc
     3181 tggataacgc actacagccg gacagagcag gtcagtttgc cgacgcactg gagaaatacg
     3241 ccgccggtct ggcaaccgca ctcagtcgtt ctcctcaaat aacgcttgaa gaaacacccg
     3301 cctcgcaggc catcctgccg accttattaa aacaggagca acacgtctgc cagcttttcg
     3361 cccgcttgcc agactggaca gccccggcat taacgcccgc cacggaacag gtacaaggag
     3421 ccacgcaatg atcaatcgtc aactttcacg cctgctgttg tgcagcattc tcggcagcac
     3481 gacgctgatt tccggctgtg ccctggtacg taaggattct gcgcctcatc aacagctcaa
     3541 accggaacaa atcaaactgg ccgatgatat tcatcttgcc agctccggct ggccgcaggc
     3601 gcagtggtgg aaacaactca atgacccgca gttggatgcc ctgatccaac ggacgctaag
     3661 tggttcacac accctcgccg aagcgaaact gcgggaagaa aaagcgcagt cgcaggccga
     3721 tttgttagat gccggttcac aattacaggt cgcagcgtta gggatgctca accgccaacg
     3781 cgtctcggcg aacggctttt taagccctta tgcgatggat gcgccagcac tgggtatgga
     3841 cgggccgttc tatacggaag ccacagtcgg tttgtttgcc ggactggatc tcgatttgtg
     3901 gggtgtgcat cgctcagcgg ttgctgccgc cattggcgcg cataatgccg cgctggcaga
     3961 aaccgcagca gtagagctat cgctgaccac gggcgtagcg cagctttatt acagtatgca
     4021 ggccagctat cagatgctcg atctgttaga acaaacacgc gatgtgattg attacgcggt
     4081 gaaagcgcac cagagtaaag tggcgcacgg tctggaagcg caagtgcctt tccacggcgc
     4141 gcgggcacag attctggcgg tcgataaaca aattgttgcc gttaaagggc aaatcaccga
     4201 aacgcgagaa tctctgcgtg cattgattgg cgcgggagcc agcgatatgc cggagatcaa
     4261 accggtggca ttaccgcaag tccagaccgg cattccggcg acactctctt atgagttgct
     4321 cgccagacgc ccggatctgc aagcgatgcg ctggtatgtt caggcgtcat tagatcaggt
     4381 ggattccgcg cgggcgctgt tctatccgag ctttgatatc aaagcgtttt tcggcctgga
     4441 ctctatccac ctggatacct tattcaaaaa aaccagtcgc cagttcaact ttatcccggg
     4501 tctgaaattg ccgctgtttg acggtggacg gttgaatgcc aatctcgaag gcactcgcgc
     4561 cgccagcaac atgatgattg aacgttacaa ccagtcagta ctgaatgcgg tgcgcgacgt
     4621 tgccgtcaac ggcacgcgtc tgcaaacgct caacgacgag cgagagatgc aggctgaacg
     4681 cgtggaagca acgcgcttta ccctgcgcgc tgccgaggcc gcctatcagc gcggcttaac
     4741 cagccgctta caggccaccg aagcccggtt gccagtactt gccgaagaga tgtcattact
     4801 gatgctggac agccgccggg tgatccaaag cattcagttg atgaaatcgc tgggcggcgg
     4861 gtatcaggca agtcccgtcg tcgagaaaaa ataaaatgtc tgccgcgtga tggctgtcac
     4921 gcggtatttc gtttcgtcac gtcaaaactg acgacagcct gtttttcgtc agagttttga
     4981 ataaatagtg cccgtaatat cagggaatga ccacaaataa aatgtggcat aaaagatgca
     5041 tactgtagtc gagagcgcgt atgcgtgatt tgattaactg gagcgagacc gatgaaaaaa
     5101 gtcgtcacgg tttgccccta ttgcgcatca ggttgcaaaa tcaacctggt cgtcgataac
     5161 ggcaaaatcg tccgggcgga ggcagcgcag gggaaaacca accagggtac cctgtgtctg
     5221 aagggttatt atggctggga tttcattaac gatacccaga tcctgacccc gcgcctgaaa
     5281 acccccatga tccgtcgcca gcgtggcggc aaactcgaac ctgtttcctg ggatgaggca
     5341 ctgaattacg ttgccgagcg cctgagcgcc atcaaagaga agtacggtcc ggatgccatc
     5401 cagacgaccg gctcctcgcg tggtacgggt aacgaaacca actatgtaat gcaaaaattt
     5461 gcgcgcgccg ttattggtac caataacgtt gactgctgcg ctcgtgtctg acacggccca
     5521 tcggttgcag gtctgcacca atcggtcggt aatggcgcaa tgagcaatgc tattaacgaa
     5581 attgataata ccgatttagt gttcgttttc gggtacaacc cggcggattc ccacccaatc
     5641 gtggcgaacc acgtaattaa cgctaaacgt aacggggcga aaattatcgt ctgcgatccg
     5701 cgcaaaattg aaaccgcgcg cattgctgac atgcacattg cactgaaaaa cggctcgaac
     5761 atcgcgctgt tgaatgcgat gggccatgtc attattgaag aaaacctgta cgacaaagct
     5821 ttcgtcgctt cccgtacaga aggctttgaa gagtatcgta aaatcgttga aggctacacg
     5881 ccggagtcgg ttgaagatat caccggcgtc agcgccagtg agattcgtca ggcggcgcgg
     5941 atgtatgccc aggcgaaaag cgccgccatc ctgtggggca tgggtgtaac ccagttctac
     6001 cagggcgtgg aaaccgtgcg ttctctgacc agcctcgcga tgctgactgg taacctcggt
     6061 aagcctcatg cgggtgtaaa cccggttcgt ggtcagaaca acgtgcaggg tgcctgcgat
     6121 atgggcgcgc tgccggatac gtatccggga taccagtacg tgaaagatcc ggctaaccgc
     6181 gagaaattcg ccaaagcctg gggcgtggaa agcctgcctg ctcataccgg ttatcgcatc
     6241 agcgagctgc cgcaccgcgc agcgcatggt gaagtgcgtg ccgcgtacat tatgggcgaa
     6301 gatccgctgc aaactgacgc ggaactgtcg gcagtacgta aagcctttga agatctggaa
     6361 ctggttatcg ttcaggacat ctttatgacc aaaaccgcgt cggcggcgga tgtcatttta
     6421 ccgtcaacgt cgtggggcga gcatgaaggc gtgtttactg cggctgaccg tggcttccag
     6481 cgtttcttca aggcggttga gccgaagtgg gatctgaaaa cggactggca gattatcagt
     6541 gaaatcgcca cccgtatggg ttatccgatg cactacaaca aaacccagga gatctgggat
     6601 gagttgcgtc atctgtgccc ggatttctac ggtgcgactt acgaaaaaat gggcgaactg
     6661 ggcttcattc agtggccttg ccgcgatact tcagataccg atcaggggac ttcttatctg
     6721 tttaaagaga agtttgatac cccgaacggt ctggcgcagt tcttcacctg cgactgggta
     6781 gcgccaatcg acaaactcac cgacgagtac ccgatggtac tgtcaacggt gcgtgaagtt
     6841 ggtcactact cttgccgttc gatgaccggt aactgtgcgg cactggcggc gctggctgat
     6901 gaacctggct acgcacaaat caataccgaa gacgccaaac gtctgggtat tgaagatgag
     6961 gcattggttt gggtgcactc gcgtaaaggc aaaattatca cccgtgcgca ggtcagcgat
     7021 cgtccgaaca aaggggcgat ttacatgacc taccagtggt ggattggtgc ctgtaacgag
     7081 ctggttaccg aaaacttaag cccgattacg aaaacgccgg aatacaaata ctgcgccgtg
     7141 cgcgtcgagc cgatcgccga tcagcgcgcc gccgagcagt acgtgattga cgagtacaac
     7201 aagttgaaaa ctcgcctgcg cgaagcggca ctggcgtaat acagtccttt ctacagcctc
     7261 ctttcggagg ctgttttttt atccattcga actctttata ctggttactc cctacccaat
     7321 cgtattatca aaatgaaaaa aattatcgca ttgatgttgt ttttgacatt ctttgcccac
     7381 gcgaacgact ccgagcctgg cagccagtat ttaaaggcag cagaggcagg ggaccgacgc
     7441 gcacaatatt ttcttgccga cagctggttt agctccggcg atttgagcaa agccgaatat
     7501 tgggcacaga aagccgccga cagcggtgat gctgatgcct gcgcgctgtt agcgcagatc
     7561 aaaatcacca atccggtcag tctggactat ccacaagcaa aagttcttgc agagaaagcg
     7621 gcgcaagcgg gcagtaaaga aggtgaagta acgctggcgc atattctggt aaatacgcaa
     7681 gcgggtaaac cggattatcc aaaggcaatt tcgctgttag aaaacgcctc ggaagatctg
     7741 gagaacgact ctgccgtcga tgcccaaatg ctgcttggtt tgatttacgc caacggcgtg
     7801 ggcattaagg ccgacgatga caaggcaacc tggtatttca aacgcagctc tgcgatttcc
     7861 cgaaccggtt attccgagta ctgggcggga atgatgttct taaacggtga agaaggtttt
     7921 atcgagaaga acaaacaaaa ggcgctgcac tggttaaatc tgagctgtat ggaagggttt
     7981 gataccgggt gtgaagagtt tgaaaaatta actaacggtt aaggtaggcc tgataagacg
     8041 cggtaagcgt cgcatcaggc attgatgtcg gatgcggcgt aaacgcctta tccgacctac
     8101 aaaatcacgc taaatcaggc tattgcgcag tctggcgtag gcctgataag acgcggtaag
     8161 cgtcgcatca gatattgatg tcggatgcgg cgtaaacgcc ttatccgacc tacaaaatca
     8221 ccctaaatca agctattgcg cagtctggcg taggcctgat aagacgcggc aagcgtcgca
     8281 tcagatattg atgtcggatg cggcgtaaac gccttatccg acctacaaaa tcaccctaaa
     8341 tcaagctatt gcgcagtctg gcgtaggcct gataagacgc ggtaagcgtc gcatcaggca
     8401 tccacatatt gccgggtgga tatccccggc aatcttcaat tattgatccg cagttttatc
     8461 aaatttgccc agcacttcgc gctcataagc cagcgctttc ttacggtcaa atttgtgttc
     8521 ccacttggca atcaccagca ccgccagcgc attacccacc acgttcagcg cagtacgcgc
     8581 catgtcgagg atacggtcaa caccggcaat aaacgccaga ccttccagcg gaatacctac
     8641 gctacccagc gttgccagca acaccacaaa cgacacgccc ggcacgccag caatcccttt
     8701 cgaggtcacc atcagcgtca gcaccagaat gatttcctgc cagatggaca ggtcaatgcc
     8761 atacaactgc gcgatgaaaa tagcggcaat actttgatac agcgtcgaac catcaaggtt
     8821 aaaagagtaa ccggtcggca ccacgaaact ggtgatcgac gccggtgctc cgtaggcttc
     8881 catcttctca ataattcgcg gcagcacgct ttcagagctg gcagtggagt acgccagaat
     8941 cagctcatct ttcagaatac gaatcaggat ccagacgctt aacccgcaca ggcgcgccac
     9001 aattcccagc actaccagcg cgaagaacag aatggcgaaa tgcaccagca gcaccagttt
     9061 cgccagtggc cacagagacg agaaaccaaa gttagccacc gtcaccgcaa tcagcgcaaa
     9121 cacacccacc ggcgcataac gcatcaccat gtgagtcact ttaaacatgg tttcagagat
     9181 ggagcggaaa acggtcacca gcggttcacg atgcgtcgcg ggcagggatg aaagccccag
     9241 accaaacagc accgagaaga agatgatcgg cagcatttcg cctttcgcca tcgacgccac
     9301 aatgttcgtc ggcaccagcg acaaaatcgt gcccataatg ccgtgggaac tgctttgtac
     9361 cgcttccgta gtgctctgat atttcgagat atcgacggtc gccagctgcg acatatccac
     9421 cccggcaccg ggctggaaga cgttcgccag agtgatcccc aaaatgatgg cgacggtggt
     9481 gatcacctcg aagtaaataa tggttttcgc gccaatacgc ccgagctgtt tggcatcacc
     9541 aacacccgcg atacccacca ccagcgtgga gatcacaatc ggcacaacaa tcattttgat
     9601 cagatggatg aagatatcac ccgccggaga gagcaaattg acgaccagcc agtcgcggct
     9661 gtcgctatgg tagtgcaggt agcttcccag gagaatgccc agcaccatag caaacagaat
     9721 ctgccaggcc aggctgaatt ttatattttt catcagaata acttcctcaa tggagcgccg
     9781 ttccccgttc agaaacgcga gaacgtgaca tactgccagg gcatgaattg tgttgcgttg
     9841 gtttgtagat ttttttaacg cttatgatca gtatcatttg cgcggtatgt tgcgcaaccc
     9901 tttaacaaca gagcttttcg ctgcattaat gcataataac gcgagtttat ggcaggatta
     9961 atgaataacc atttgttatg gaaagatttt tatttgttta atctgcaata atactgattg
    10021 gtgattattt ttggcatatg ttcattcgat tattttttat gcagctcaat aacataatct
    10081 tcatcaccag atgattaatc tgatctccgc tgcaacaatt tcgccatatt aatcgactcg
    10141 accagctgtg tccggttgat ccggggtgag ttgagatcca tcactttttg ccatagttcg
    10201 atggcctgcg cgtaattcgc ctgcataaac gcatcggaag ccagcagcat cagagcggtg
    10261 atttcgttag agtccagcgc gagggcttta tcgatcattg cgcgtgtctg ggcggtcata
    10321 tgctggctgg cctggtaata aagcaccgtc gccagcgccg catacagttc agcgttctca
    10381 ccacgtagtt gcaacgcctg acggtacgcc agcagcgaat tgctgtaatc attttgccac
    10441 agatagtact cgcccagtaa cgcccactgt tcgctgtttt gtggattagc gcggatttta
    10501 tcctgcaatg cctgaagctg cgcttctggg gtttgctggc tggcaaactg atgcagcgga
    10561 tcgcgctgac gctgatactc cgcacgtacc gcctgccatt ttgggcttaa cagataactg
    10621 ccgatacaga gaaatgccat caaaatcgtg agtgttgtca gcattttcac cggtatttgt
    10681 gactgtttca tcgcttcgcc ctcactcgcc agaggatcag tgccatcagc agtaacaaca
    10741 ccactggcag cgcccacaac accagcgtct ggcccgttaa cggtgggtta tagcgaacaa
    10801 aatctccgta gcgttcggtc atccagccga tgatttcagc ttcgttcttc ccctccgcca
    10861 ccatgctgta aacctgatgg cgcatactga ccgccaccgg cgcgttggat tccagtaagt
    10921 tttgattctg gcactgcgga caacgtaact ggctggcaat atttaacgcc tgttgctgtt
    10981 gttgcggatt ggcgaattgc caggtgtcta cgacctgagc gcgcgcaaaa caggtaaata
    11041 acagcagtag cgtgagaagt cctttattca tcgcgcttcc tcccccgcca tccgcttaac
    11101 aatgcgcccg caatcatcaa caatcctccc ccccagatcc agcgcacacc gctttgtaca
    11161 tacaaacgaa aagcgtaacg atccgctccg gttttctcgc ccatgaccgc ataccagtca
    11221 tggatgccgt tccagcgaat tgacggttcc atcatttgct gacggcgtgc ttcataaaaa
    11281 cgccgctccg gcgtcagttc accaatgcgt tgctgatggt caaacagcgc cactatcgct
    11341 ttttcgctgg tgtaattgcc tctggcttgc agatcgagtc gctcaaaacg gaaggtgtat
    11401 cctgccagcg tcacctgctg acccggctgt aaattcaggc tgatctcctg acgactgacg
    11461 ctggaaacca cgatccccgc ggcaaataac agcacgccag catgagctac cagcgccgga
    11521 agctgcacgc gtttgccaga gacaaacgtc gccagcacaa tcaccaccag catcaacaga
    11581 ccaaacggta acgtcgcgcg gttaaaatac ggcgcgccaa cggagaggcg tccccagccc
    11641 agcagaccgt aaatcatcgg ataaagcgtt cccaccagca cgatcagtag cactgcgcaa
    11701 aacagcaaca gcgtagcgag gattaacatt tcccgcgata accccgaaaa acgcaccgcc
    11761 gggccaccgt cccgcgctcg ccagccatac agagccagag acgcaaggct aatcagtgca
    11821 aacaggctga acaacggcac ggcgcggacg ttatccagtg cgaacgcatg aaccgaaacc
    11881 agaatgccag aacggacaat taaggtgccc agcagcgaca gcatcagagt aactatcgcc
    11941 agcagcagcg accagtggcg gaaaatcccc cgctggcgtg tcagggacaa actgtgcagc
    12001 agcgcagtgg cagaaagcca gggtaataaa gaggcgtttt ccaccggatc ccagaaccac
    12061 cagccgcccc agcccagttc gcaataggcc caccaggaac cgaggatgat cccagccgtt
    12121 aatgcactcc agccaggtaa cgcccagcgc cagcaaattc gggcgcaggc accatcaaac
    12181 tcgccgcgca gtaaactcgc cagcgccacg ctcgccgcca ccatcaaacc gccatagcca
    12241 aggtaaagca gcggtggatg aaagataaga ccgggatgtt gcagcatcgg attgagatcg
    12301 cggccttcga ttgctggtgg aaatatccgc acgaagggat cggaccacag cactacaaac
    12361 agtagcagtg cggcgagcat gagagataaa acggctagcg ttagcggaaa tagcggatcg
    12421 gtttgctgcc gataatgcca ggcaaacagc gcgctccagg cggaaagcag cagcacccaa
    12481 agcagcagcg aaccttcatg accgccccac accgccgcca gtttgagttc ccacgacagc
    12541 aggctgtagc tatgttgggc gacataaatg accgagaaat cgctgatgag aaaacaatac
    12601 gtcagtacgc caaaggcgag cagcaggagc gcgaactgcg ccagaatgcc gatgcaagtg
    12661 agtcgcatca tggcaggcca gcgcaaccgc actcccgcga aggccgtcaa cggggtcaac
    12721 acgttgaccc cgagacttaa caacaacgcc agaaaaccga cttcaggaag gaatacgtcc
    12781 agaaaccacc tcttttctgg cttacgccac cgtcaactgt ccggcgtaga gaatgaaaaa
    12841 gcgcagcatc aacacgccga ccagactggc cccacacgcc gccagcacgg taggaatgcc
    12901 ggaactgcga ttgacccacg gtttgagcaa cattggcaca atcagcccca gcccggcgac
    12961 accaagccag aaccaccacg tccagaaacc gccacctaat gccgccacca gcgcacgcac
    13021 tttaccgtca tcgcccagcg ccagaccgac aaaaaacgcc accagcagga agatttcacc
    13081 ccataccaca gggatttcca tacggtgaac aaagtgcgct tccgtggaat gcggattttt
    13141 gcgttggcgt atcgccatgg cgatcagcgc taccgccgca ccggacgaga tgccggagaa
    13201 gaggaacagc accggcagga tcgggttatt gaggaacgga tacgatttca gcgccgacag
    13261 cagaaagccg gtataagccc ccagcagcac cgccagcacc agcatcaatg tttccagtcc
    13321 gcgatgaacg ggcgtcagca ggctcagaac cttttgcacg atccccagct tcggcaacca
    13381 gcgttgttgc agggcaagca aatcatgttc aaagattttc gccagccaca gcaccagcac
    13441 caccatgtag agctgaaaca acatcacccc catcgacatc accgaggtaa aactgtagtg
    13501 gaacatcagc ttccagaagg tccacggctt tgtcaggtgg aagacgagga tcaacagacc
    13561 gaggatcacc gcgccaggcc cgacaattag cgtggtgcgc agcaacgtac tgtctgcacc
    13621 gcccgcctgc gggtagaagc gacgtaacag cacggccagc gtcaccagac cggcagaaat
    13681 accaatcaaa aacaggtaga tggcaatcgg ccagtcccac accagcgatt caaaatgaaa
    13741 tgcggaagtc tgcatcattg gctcacctcc ccgtatttaa acggtacgcg gtacagcttc
    13801 ggtttggttc ccagcgccag cttgtagcgg taagtgggct tctggcgcag cagttgcgaa
    13861 atctcactgt tgggatcgtc cagattgcca aacgtcagtg ccttggtcgg gcaagcttca
    13921 acgcacgcgg gcagcttacc ggcctgcaaa ttggtcttac ggcagaaatc gcatttgtcc
    13981 gccgtcttcg tgaccggatg gataaagcgc acgcggtacg gacaggcggc gatgcagtac
    14041 tgacaaccga cgcagagatc aggattaaca tcgacaatcc cgctagcggc atcacgaaaa
    14101 gacgcaccgg tcgggcagac gtcaacgcac ggcgcatgat cgcagtgctg gcaagactta
    14161 cggaagaagc gatatttcac atcaggaaat tcgccctgcg gctcgctacg aataatcgtc
    14221 aggcgcgaga cgccttccgg caccttgttc acttcccgac aagcatccat acaggcggta
    14281 cagccgatgc ataacgactc gtcatgcacc atgccgtaac gcaccccatt gatattcaac
    14341 gttttcgcca ccacgcgccc tgcggtgccg ctgacggctg ccagcacgcc gacgccggtg
    14401 agaaactggc gacgagacca ggtcatggct gctccttaag caacggaaca gacgccgggt
    14461 taaagttcgg attggtgcgc tgatcgctgt ggcaatcgac gcaaatctta atccgtcctt
    14521 tgtcacttaa cgtctgcatc gtatcttgct gtggatgcag ggaatggcag ctggcgcacg
    14581 ccactttggt tacgtggaca tcgtgtggcc agaacgcttt ttgcaactgt tccggcagat
    14641 ggcaggacat acagacgctg ttctgctctc caaccttgta catcggctcg ttaaagcgca
    14701 tcacatcttt caccccttcg cggtgttgcg gtgatggctg accgtggcag ttggtgcagg
    14761 tgaccggcag tttgttattc gggttgataa cggaagcatg tttgccatgc ataccttcgg
    14821 tatctggctt atgacagtcc agacaggcgg catccgggtt acgctgctgg gtaacttcgt
    14881 agcgatcacc agatgcctgc gccgcagggg tagcggtaat tccgttcagg ctccacaaca
    14941 ggcccgacgc cagcaccccg gcagttaata acgaacgtaa tacgctcata ttcactccat
    15001 tgtgttttcc accccttgcg aggcggaacg gggttattgg cttaacagac cgtttttacg
    15061 tgcctgctct tcccactgcg ggatcaccgt tttgatgaag tcctgcttct cggccttgat
    15121 ttgctccatg ttcaggccaa tggcctgctg ggctttctct ttggtcgaga tatccgggat
    15181 ctggatttca tgggtaatgc ctttggtcgc cagcaggcgc gccagtttgg tgcgtgcatc
    15241 cgccgcttta tccatcgccg taccgagcat cctcagacct tcttccggtg cgtgcatatg
    15301 aatgccgtgg gaagcgatcg ccaaatccca acgccactgg gcatgacgga tatcgtcctg
    15361 aatcggcttc atttcggctt ccgttgcgcc tgcatccagc gccgctttcg cttcgaagtg
    15421 agcgtgaacc agttgatctt caaccttgat tttcaggtcg ttaatcgact gcttacgttc
    15481 cgcgaccact ttttgcaagg cagctttgtc ctgggtatgg cagttcgcac aagtctgggc
    15541 gaagttatca aacggattac caattttatg gtcggtgtag agtttgcctt cggcgttctg
    15601 cacttttggc atatggcaat cgatacaggt cacgttgttt ttgccgtgaa tgcccgctgt
    15661 ccaggtttca tattccgggt gctgcgcttt cagcattggc gttttcgaca gggagttagt
    15721 ccagtcagag aaggcaattt tgtcgtaata ctgctccata ttttcgactt tcatgccgtc
    15781 atcccacggg aatttaaccg ctttgttttt gccgtcgaag taatactcca catggcactg
    15841 accgcaaacc atcgattgct ggtcgaaacg tccggctttc tcaaaaggtt taccaatggc
    15901 ttccatcgcg cgagccgcat acggacggga aagggttaac tccggtttgc ctttggcgaa
    15961 ctctggtgag gcggtgttat ggcaatcggc acaacctaag ttgttgacga tttccgggcc
    16021 gccgcgcgcc cattttccgt ggaagtagcc atcttcgccg tctttctgga tcagacgcgc
    16081 cacatctggg cttttacaac tccagcatgc catcggtagc ggaccatctt cagcgttttt
    16141 cggcgcaccg gtacgcaggg tttcacgcac atcggtcaca gcaaaggcat gtccacgcgg
    16201 cttgttgtaa tcgcgcgaga agggataccc cgcccacagg atcaccagcc gtggatcttc
    16261 cgccagggcg tcaacacgct ctgactgttc cgaggtggct ttccaggaga gatattgatc
    16321 gggatgttgc ggggcaaagg tttcattctt cgcttcaaca gttacaggtt ttgcaggagc
    16381 tgccgtttgt tcagcgtgaa cagaagtgaa aaagaaaaaa ggaatcaata agctgaagat
    16441 acggcgtgcg tttattttta tccttgtcat aggggcttca tccgaattgc gccattgttg
    16501 caatggcggt ttttattgtt tttcacgaca gtaaccgcac ctacactgtc atgacattgc
    16561 tcgcccctat gtgtaacaaa taaccacact gtgaatgttg tctttaatca attgtaagtg
    16621 catgtaaaat accactttag agttagtcag tatcttcctc tttttcaaca gcatgcataa
    16681 ctgcatgttc ctcaaagaat taatcaactt ttgttgctga ccttcaaaaa ttaccctgcc
    16741 gtttatttgc acaattctac ttttgcgtga tctgtcgccc aaatactaaa caaaactgcc
    16801 aataccccta catttaacgc ttatgccaca tattattaac atcctacaag gagaacaaaa
    16861 gcatgagtca aattcacaaa cacaccattc ctgccaacat cgcagaccgt tgcctgataa
    16921 accctcagca gtacgaggcg atgtatcaac aatctattaa cgcacctgat accttctggg
    16981 gcgaacaggg aaaaattctc gattggatca aaccttacca gaaggtgaaa aacacctcct
    17041 ttgcccccgg taatgtgtcc attaaatggt acgaggacgg cactctgaat ctggcggcaa
    17101 actgccttga ccgccatctg caagaaaacg gcgatcgtac cgccatcatc tgggaaggcg
    17161 acgacgccag ccagagcaaa catatcagct ataaagagct gcaccgcgac gtctgccgct
    17221 tcgccaatac cctgctcgag ctgggcatta aaaaaggtga tgtggtggcg atttatatgc
    17281 cgatggtgcc ggaagccgcg gtcgcgatgc tggcctgcgc ccgcattggc gcggtgcatt
    17341 cggtaatttt cggcggcttc tcgccggaag ccgttgccgg acgcattatc gattctaact
    17401 cacgactggt gatcacttcc gacgaaggcg tgcgtgccgg gcgtagtatt ccgctgaaga
    17461 aaaacgttga tgacgcgctg aaaaacccga acgtcaccag cattgaacat gtggtggtac
    17521 tgaagcgtac tggcgggaaa attgactggc aggaagggcg cgacctgtgg tggcacgacc
    17581 tggttgagca agccagcgat cagcaccagg cggaagagat gaacgccgaa gatccgctgt
    17641 ttattctcta cacctccggt tctaccggga agccgaaagg cgtgctgcac actaccggcg
    17701 gttatctggt gtacgcggcg ctgaccttta aatatgtctt tgattatcat ccgggtgata
    17761 tctactggtg caccgccgat gtgggctggg ttaccggaca cagttatttg ctgtacggcc
    17821 cgctggcctg cggcgcgacc acgctgatgt ttgaaggcgt accgaactgg ccgacgcctg
    17881 cccgtatggc gcaggtggtg gacaagcatc aggtcaatat tctctatacc gcgcccacgg
    17941 cgatccgcgc gctgatggcg gaaggcgata aagcgatcga aggcactgac cgttcgtcgc
    18001 tgcgcattct cggttccgta ggcgagccaa tcaacccgga agcgtgggag tggtactgga
    18061 aaaaaatcgg caacgagaaa tgcccggtgg tcgatacctg gtggcagacc gaaaccggcg
    18121 gtttcatgat caccccgctg cctggcgcta ccgagctgaa agccggttcg gcaacacgtc
    18181 cgttcttcgg cgtgcaaccg gcgctggtcg ataacgaagg taacccgctg gaaggggcca
    18241 ccgaaggcag tctggtgatc accgactcct ggccgggtca ggcgcgtacg ctgtttggcg
    18301 atcacgaacg ttttgagcag acctacttct ccaccttcaa aaatatgtat ttcagcggcg
    18361 acggcgcgcg tcgcgatgaa gatggctatt actggatcac cgggcgcgtg gacgacgtgc
    18421 tgaacgtctc gggtcaccgt ctgggaacgg cggagattga gtcggcgctg gtggcgcatc
    18481 cgaaaatcgc cgaagccgct gtcgtcggta ttccgcacaa tattaaaggt caggcgatct
    18541 acgcctacgt cacgcttaat catggggagg aaccgtcacc agaactgtac gcagaagtcc
    18601 gcaactgggt gcgtaaagag attggcccgc tggcgacgcc agacgtgctg cactggaccg
    18661 actccctgcc taaaacccgc tccggcaaaa ttatgcgccg tattctgcgc aaaattgcgg
    18721 cgggcgatac cagcaacctg ggcgatacct cgacgcttgc cgatcctggc gtagtcgaga
    18781 agctgcttga agagaagcag gctatcgcga tgccatcgta acccacaatt gcccgatgcg
    18841 agtcggtaac ggtttgtagg cctgataaga cgcgacagcg tcgcatcagg cgctggttgc
    18901 cggatgcggc gtgaacgcct tatccggcct acattcggca acggctgtag gcctgataag
    18961 acgcgacagc gacgcatcag gcgttgattg ccggatgcgg cgtaaacgcc ttatccggcc
    19021 tacattcggc aacggctgta ggcctgataa gacgcgacag cgtcgcatca ggcgttgatt
    19081 gccggatgcg gcgtgaatgc cttatccggc ctacattcgg caagggttac ccaagcgtta
    19141 accttctccc ataagggagc gggaattaaa acaatcccta cattacctct ggagaatctg
    19201 tgatgaatgg tacgatttat cagcggatag aagacaatgc gcatttcagg gagttagtcg
    19261 aaaaacggca acggtttgcc accatcctgt cgattattat gctggcagtt tatatcggct
    19321 ttattttact gatcgccttc gcgcccggct ggctgggcac cccgctgaat ccgaacacca
    19381 gcgtcacacg cggtattccg attggtgttg gagtaattgt gatctccttt gttctcactg
    19441 gtatctacat ctggcgggcg aacggcgaat tcgaccgtct taataatgaa gtcctgcatg
    19501 aggtacaagc atcatgaaaa gagttctgac ggcgcttgcc gccacactcc ctttcgcagc
    19561 taacgccgcg gatgctatta gcggggccgt agagcgccag ccaacaaact ggcaggcgat
    19621 tattatgttc ctgattttcg tcgtgtttac gctcggcatt acctactggg cgtcaaaacg
    19681 cgtacgttcg cgtaatgact actacaccgc aggcggcaat atcaccggtt ttcagaatgg
    19741 gctggcgatt gccggggact atatgtccgc cgcctcattc ttggggatct ccgcgctggt
    19801 gtttacctcc ggctatgacg gcttaattta ctcgctgggc ttcctggtag gctggccaat
    19861 cattctgttc ctgattgccg aacgtctgcg taacctgggc cgctacacct ttgccgatgt
    19921 agcctcttac cgcctgaaac aagggccgat tcgtattctt tcggcctgtg gttctctggt
    19981 ggtggtggcg ctttacctta tcgcccagat ggtgggcgca ggtaaactca tcgagctgct
    20041 gtttggcctt aactatcaca ttgcggtggt gctggtcggc gtgctgatga tgatgtacgt
    20101 cctgttcggc ggcatgctgg cgaccacctg ggtacagatt atcaaagccg ttctgttgct
    20161 gttcggtgcc agctttatgg cctttatggt gatgaaacac gtcggcttta gcttcaacaa
    20221 tctgttcagc gaagcgatgg cggtacaccc gaaaggtgtc gatatcatga agccaggcgg
    20281 gctggtgaaa gatccgatct cagcactgtc gctggggctc ggactgatgt ttggtacggc
    20341 gggcttgccg catattctga tgcgcttctt tacggtcagc gatgcccgcg aagcacgtaa
    20401 gagcgtgttc tacgccaccg gatttatggg ctacttctac atcctgacct ttattatcgg
    20461 cttcggcgca atcatgctgg ttggtgcgaa tccggaatat aaagatgctg cgggccatct
    20521 gattggtggt aacaacatgg cggcagttca cctggccaat gcggtgggcg gcaacctgtt
    20581 cctcggcttt atttcagcgg ttgctttcgc cactatcctc gcggtggtag cggggctgac
    20641 gctggcgggc gcatcggcgg tgtctcacga cctgtacgct aacgtcttca aaaaaggcgc
    20701 gaccgaacgt gaagagctgc gggtatcgaa aatcaccgtt ctgattttgg gtgtgattgc
    20761 cattatcctc ggcgtgctgt ttgagaatca gaacatcgcc tttatggtgg ggctggcgtt
    20821 tgccatcgcg gcgagctgta acttcccgat cattctgctt tccatgtact ggtcgaaact
    20881 gactacccgt ggcgcaatgc tgggcggctg gttaggactg attactgcgg tggtgctgat
    20941 gatcctcggt ccgacgattt gggtacagat cctcggtcac gaaaaagcca tcttcccgta
    21001 tgaatacccg gcactgttct ctatcagcgt ggcattcctc ggtatctggt tcttctcggc
    21061 aacggataat tcagcggaag gtgcgcgcga gcgtgaactg ttccgtgcgc agtttatccg
    21121 ctcccagact ggctttggcg ttgagcaagg ccgcgcgcat taatctccac tagcctctgg
    21181 tcacctgacc aacggtaaaa aggtgcgatg tactgcacct ttttaccgat tacaccgcca
    21241 aagcgttatt ggttctcttc aatcagcaac gcttcaagca aatcgagatc gtgcagtaat
    21301 ttttgcagcg tttcgttact gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt
    21361 tcagaacgca atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt
    21421 tcaacgtcgt tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactaacc
    21481 tctgtgagca gctggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt
    21541 ttctggatcg ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcctctttc
    21601 aattgttgcg aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg
    21661 ccgacaaaca gcgagaagag aatgacacca gccgccagga acaccagctc atagcgcgcc
    21721 gggaagacgt taccatccgg caagagcagc gggattgaga gcacaccagc cagagtgatc
    21781 gccccacgca ccccggcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac
    21841 tccatcggct tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg
    21901 aaacggacca gcatcagcgc cgcataaatc agaatgatat cggtaaacag catccagatt
    21961 tcgacgttag ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagctgc
    22021 agacctaaca gcaggaatac catgccgtta aagacaaatt ccagcatcgc ccaggtactg
    22081 tttgcgcgca ggcgcattgc cagcggcgca cggcgcatca caccggaacg ggtgatggtc
    22141 atcccggcgg caaccgcagc gaggataccg gaaacgccaa tatgttcggc aatcagataa
    22201 gaagcgaatg gcagcaggaa cagcaggacg atctgcgtcg cgggttcatc accgccccag
    22261 cggctgagga atcgcagcga gcgaccgtac agccagctca ccacaaaacc ggcgagaata
    22321 ccgccaatgg ccactttcat aaattcgacc gtcgcaccac cgacggtgaa gatcatcgtc
    22381 cccatcgcca ctgccacggc aaacttcaac gacaccagac cggaggcgtc attcatcaac
    22441 gcttcgccct gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccc
    22501 gagagcgcca cagcatcggt cggagaaagc accgccgcca gcgcaaaggc ggggatcagc
    22561 ggaatgcccg gcaccaccca gtaaataaga aagccaatgc cgaccacggt gaccaccacc
    22621 agcgccagcg cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt
    22681 ttccagccat cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca
    22741 aactccacat gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata
    22801 agcggaagcg ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg
    22861 agggtcatta tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt
    22921 atgatctgag aaaggcgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca
    22981 cacaatgtgc ggagaaaata aaaacgggca gccattgcca cccgttttca gaatcatatg
    23041 aaagattaga tagcccagcc acccgcatag aaggtcacca gcgccacagc gataacgacc
    23101 gtaccgatgt tcaacttgcg ccattcgccg gaaaccagac gaccaatcac cagagtcgcg
    23161 aagccgatca tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc
    23221 agacccgcca tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt actcagcatc
    23281 agcaggccaa cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagagagc
    23341 ggagagagga acagaatcag caggaacagc acgccaacgg tgatagccgt caaaccggtt
    23401 ttaccgcccg ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc
    23461 accaggccag agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg
    23521 atctgcccat ctttatccag caggttcgcc tggccggcga cggcacggat agttccggtg
    23581 gcatcaaata ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc
    23641 gcgcccataa tgtccaggct gccaattagt gaattgccgt tttcatcgct caatgaaggc
    23701 atggcgaaaa cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata
    23761 ccgataatgg tcagcagaat gccaccaggg actttcagtt tttccaggcc gataatcact
    23821 gccagcccca ccaacgacat catcaccgga aaggtcgtga aatcccccag cgcgacgggc
    23881 agaccatcaa gtgggttttt aatcaccaga ccgacaccat tagcagcaat cagcagcagg
    23941 aacagaccga taccaatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc
    24001 cagctacgga tacccgtggc agaaatgacc gtaaacagca cacccatcag gaacacggca
    24061 cccagcgcga caggtacgct aatatgttgc cccagcacca gactgaatgc ggtaaacgcc
    24121 gtcagggaga tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata
    24181 gaaccgagtc cggcaaccag acaggtcgca acgaaaactg ccgcaggcgg gaagcctgct
    24241 ttacccaaca tgcctggaac gacgataacc gagtagacca tcgccagaaa tgttgttaac
    24301 ccggcaacca cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg
    24361 agtgaaccgc cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa
    24421 ttttcgtcaa gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca
    24481 ggtttgcgac aaattggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac
    24541 gggtggtaaa aaaagcaaac gattatccgg cgatcatagc ggctttttca accgaagttt
    24601 gctgcttttt tctcttttat tgtcttgacg atgaaaaacc aggcaattga tgcgcaaacg
    24661 ttatcgtcga tgcgcaagat tgcagcgtta tcgagccgta taccgttcag gattgcgata
    24721 aaaaatcgtc tgagatgaca aaaatattgc ggctaaaaag tgtactttta cactggcgaa
    24781 gaaacgccgg tgacatacaa ttaaagcatc aacaccaacc ggaacctcca ccacgtgctc
    24841 gaatgaggtg tgttgacgtc gggggaaacc ctcctgtgta ccagcgggat agagagaaag
    24901 acaaagaccg gaaaacaaac taaagcgccc ttgtggcgct ttagttttgt tcatcttcca
    24961 gcaagcgtgc gcctgtgcct tcttctccta agcggtcgcc cgggttacgc aatgggcaat
    25021 cactgcgcga aaggcagcca caaccaatac atccgtccag ctcgtcacgc agcgccacta
    25081 aggtatgaat gcgccgatcc aactcttctc gccattggga cgaaagctgt ttccactctt
    25141 tcgcacttaa tgtatgccct tcgggcaaca cgccaaatgc ttcacgaatg gtcgccagcg
    25201 gaatgccaat acgctgagca attttgatga ttgcgacata tcgcaacaca tcacgtttat
    25261 atcgccgctg attgccacta ttacggatac tgctaatcaa ccctttgctt tcatagaaat
    25321 gcagcgccga taccgccaca ccgctgcgtt ttgccacttc gccgggggtt agcagcgctt
    25381 taatgcgggg taatttcttt tccataaatc gctttacctc aagttaactt gaggaattat
    25441 actccccaac agatgaatta acgaactgaa cactgaaaag aggcagattt atgtcccatc
    25501 agaaaattat tcaggatctt atcgcatgga ttgacgagca tattgaccag ccgcttaaca
    25561 ttgatgtcgt cgcaaaaaaa tcaggctatt caaagtggta cttacagcgg atgttccgta
    25621 ctgtgacgca tcagacacta ggtgattaca ttcgccaacg ccgcctgtta ctggccgccg
    25681 ttgagttgcg caccaccgag cgtccgattt ttgatatcgc aatggacctg ggttatgtct
    25741 ctcagcagac cttctcccgc gttttccgtc ggcagtttga tcgcactccc agcgattatc
    25801 gccaccgcct gtagttttat tgcccgcgcg ttaactcccg cgcgggtaat tgctccatcc
    25861 attgcataaa cacctgcggc ggcatcgcct tcgcaaagaa ccatccctga caatagcgca
    25921 cgccgcgttt gcgcagccag ttaacctgct cctcagtttc gacgccttca gcgatcgttt
    25981 ttaaccccag actgtgcgcc agctcgatga tatgttccgc aatcaaatga ctggttttat
    26041 gggtggtcag cgtctcaaca aacgatttgt cgattttcaa aatatcgaca ttcaatgatt
    26101 taaggttatg caagttagag tagccaatac caaaatcatc aattgccact tcgtaaccag
    26161 cctggcggaa agccagaata atcggcgtca ttttgtcgac atcaagaaat gcatgttcag
    26221 tcacttcaaa tttaatttgc tgcggacgca ccgcatattg ctctgttttc tggttggttc
    26281 gcgctatcaa ccgtgacgta tgaaaatcgg aggccgacag gttaatagaa acatagcgat
    26341 cgacatgtgt tgccaggtac acgcccagat cgcggaagac attatcaata acataatcag
    26401 ttatctgttc gatcatcccc tccttttctg ccagcggaat aaactctgcc ggattcatta
    26461 cttgcccctg ctcaccaggc caacgtaaca atgcttcagc tccgatacat ttttctgttt
    26521 tgatatcgat tattggctgg taataaaggc aaagttgatg tttttcgagg gcgcgttgca
    26581 atttgcgttt gggagacaaa tagttttgtc gaatacgtag ccagagtaat agcagaacaa
    26641 gactccccaa aataccggcg ggcaacgcaa aaataagatg attataaaaa tgagttataa
    26701 gacgttgata tgaagtcgca acaatggctg caattgggcg ttttgtcgaa taaactgtcg
    26761 catataaata gccatttcgt tgtacggtta aatcattcaa atgaatcagc ggagaaaacg
    26821 ttgctgccga ggcctcgttg cttaacgaga aaaaggtttt cgtcaccgta tcatacactc
    26881 cccattgcaa tgtcggatca tcagacatca cttcactcca gaagagaggg ttgataaccg
    26941 ccacataatt tccccgctgc atgtaggtca ttttatagcc agagaaaaaa ggcgtatcac
    27001 ggtaataata gatagaaacg ttaggttcac gcttataatc ggccggtgca atcgtatagc
    27061 catttacaga cgctatcagc gatgagcata aaaaatggtt atcacgggca tagatcaatt
    27121 cattaatata aagatagcca cgaataatat tcaacattcg cttttgatgg gctggagtgc
    27181 aaacttgccc ctgatagcgt tctgctgcat cgctcactaa atctgcctgg cgaatgacca
    27241 gctcggattt atccagcgcc agctgagcaa aagtttggag ctgactattc acttctgatt
    27301 ttgcccaaag gaaggcaatc catagcgaca gaatgatggg aaagagaact aaaaagataa
    27361 tgcccggcaa cgccagtaat tggtgtcgtg cacgatgact cattgctatc aatttcctgc
    27421 ctgtttatac ccggccattg caaggtgaaa accctgggca ccgacgtctt tagataaatc
    27481 ttacggtata aatagcactt ttgcctgatt tgaaattgat acctttttaa tggtcacctg
    27541 gataaaaata attcattgat attcaaaact aattttctac aggaatgcag aggcggcggg
    27601 aagatatgcc cttaatgcca ctgaaaaacc acataatctg tacagcaata aaagtcacgg
    27661 cctaatctgg ctaacaaaca atcaattgtg atatagttca caaaattaat gaaacaaaca
    27721 gagtgtttca tttttgtgtc ctgcaagccc cgcgatagcc agagtgtacc acgcctcccg
    27781 tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag aggaattgtt tgatggctac
    27841 cctcaccact ggcgtggttc tacttcgctg gcaacttctt agtgccgtaa tgatgtttct
    27901 ggccagcacg ctcaacatcc gttttcgtcg gtctgattat gtcgggcttg cagtgatcag
    27961 cagcggtctg ggcgtggttt ctgcctgctg gttcgcaatg gggttgcttg gcatcacaat
    28021 ggcggatatc accgccatct ggcacaacat cgagtcggtg atgattgaag agatgaatca
    28081 gacaccgcca caatggccaa tgattttaac ttgatatgta gaagcctcca taatggaggc
    28141 ttcttttttt atggctggca gatgctttaa tcatccacct taaaacaata taacctattg
    28201 ttttaatgac aaatcagaac ggaatatcgt catcaaaatc catcggcggc tcgttagacg
    28261 gtgctgccgg agcggactgc tgcgggcgag actgcgcgcc gccgctgaac tgattgccgc
    28321 cctgcggctg ctgaggctga ccccaaccgc cctgcggctg accaccaccg atattgccac
    28381 ctgcaggagc gccaccaccc tgacgaccac ccagcatctg catggtgccg ccaacgttca
    28441 ccacgacttc tgtggtgtag cgatcctgac cggattgatc ggtccatttg cgggtacgca
    28501 gctgaccttc gatataaacc tgagaacctt tacgcagata ttcgctggct acttctgcca
    28561 gtttgccgaa cagcacaacg cggtgccact cagtctgctc tttcatctcg ccggtcgctt
    28621 tgtcacgcca ggattcggaa gtagccagcg taatgttggc aacagcgcca ccatttggca
    28681 tgtagcgtac ttccgggtcc tgacccagat taccaacgag aataaccttg tttacgcctc
    28741 tgctggccat gttcgtgtct cctgaaaaaa aatcgttctg aataagtgta aacgcgcgat
    28801 tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga aacaaacctg
    28861 gccagacatt gttacacaac actccgggta atgcattcca atactgtata ttcattcagg
    28921 tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca ccgggcaaaa
    28981 aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc ccgcacccat
    29041 aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt gaccgggctt
    29101 tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg gcagcgccgt
    29161 tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga gaagccggac
    29221 gtcgaccata ttgaggggct ttctcctgcc atctcaattg agcagaaatc gacgtctcat
    29281 aacccgcgct ctacggtggg gacaatcacc gaaatccacg actatttgcg tctgctgtac
    29341 gctcgcgttg gtgagccgcg ctgcccggac cacgatgttc cgctggcggc gcaaaccgtc
    29401 agccagatgg tggataacgt gctttcgcag ccggaaggca agcgtctgat gctgctcgcg
    29461 ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggaaaatct ggcaagccag
    29521 ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc gccgaaactg
    29581 gaactgcaaa agaaacatac cattgaagtg gtggttgatc gcttcaaggt gcgtgacgat
    29641 ctcacccaac gtcttgccga gtcgtttgaa accgcgctgg agctttccgg tggtaccgcg
    29701 gtagttgccg atatggacga tccaaaagcg gaagagctgc tattttccgc caacttcgcc
    29761 tgcccaattt gcggctacag tatgcgtgaa ctggaaccac gcctgttttc gtttaacaac
    29821 ccggcgggtg cctgcccgac ctgtgacggc cttggcgtac agcaatattt cgatcctgac
    29881 cgcgtgatcc agaatccgga actgtcgctg gcaggtggtg cgatccgagg ctgggatcgc
    29941 cgcaacttct attacttcca gatgctgaaa tcgctggcag atcactataa gttcgacgtc
    30001 gaagcgccgt ggggcagcct gagcgcgaat gtgcataaag tggtgttgta cggttctggc
    30061 aaagaaaata ttgaattcaa atacatgaac gatcgtggcg atacctccat tcgtcgtcat
    30121 ccgttcgaag gcgtgctgca caatatggag cgccgctata aagagacgga atccagtgcg
    30181 gtacgtgaag aattagccaa gtttatcagc aatcgtccgt gcgccagctg cgaagggacg
    30241 cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata cgccgctacc tgctatctcc
    30301 gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc aggtcagcgg
    30361 gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt cctcgttaac
    30421 gtcggcctga attacctgac actttcccgc tcggcagaga cactttctgg cggtgaagcc
    30481 cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat gtacgtgctg
    30541 gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgctggg tacgcttatc
    30601 catctgcgcg atctcggtaa taccgtgatt gtggtggagc acgacgaaga cgcgattcgc
    30661 gccgctgatc atgtgatcga tatcggtccg ggtgcgggtg tacacggcgg tgaagtcgtc
    30721 gcggaaggtc cgctggaagc aataatggcg gtgccagagt cgttgaccgg gcagtacatg
    30781 agcggtaaac gcaagattga agtgccgaag aagcgcgttc cggcaaatcc ggaaaaagtg
    30841 ctgaaactga caggcgcacg cggcaacaac ctgaaagacg tgacgctgac gctgccggtg
    30901 ggtctgttta cttgcatcac aggggtttca ggttccggta aatcgacact gattaacgac
    30961 acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga accggcaccg
    31021 tatcgcgata ttcagggact ggaacatttc gacaaagtga tcgatatcga ccaaagccca
    31081 attggtcgta ccccacgttc caatccggca acctataccg gcgtgtttac acctgtacgc
    31141 gaactgttcg caggcgtacc agaatcccgt gcgcgtggtt atacgccagg gcgtttcagc
    31201 tttaacgtcc gtggcggacg ctgcgaggcc tgtcagggcg acggcgtgat caaagtggag
    31261 atgcacttcc tgccggacat ttacgtaccg tgcgaccagt gcaaaggcaa acgctataac
    31321 cgtgaaacgc tggagattaa gtacaaaggc aaaaccatcc acgaagtgct ggatatgacc
    31381 atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcacgtaa gctgcaaacg
    31441 ctgatggatg ttggcctgac gtacattcgc ctcgggcagt ccgcaaccac actttctggt
    31501 ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac cgggcagacg
    31561 ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca gcaactgctc
    31621 gacgtactgc ataaactgcg cgatcagggc aataccattg tggtaattga gcacaatctc
    31681 gacgtgatca aaaccgctga ctggattgtc gacctgggac cggaaggcgg cagtggcggc
    31741 ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcag agtgcgaagc ttcgcatacg
    31801 gcgcgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttcagagcg gccttcttct
    31861 ttcagagttg caccagcaat ttacgttttt cttccggcag caaattcacc gcctgctgat
    31921 aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg agatacacgg
    31981 tgctccagtg cgctttattc agatggcggc ttggacgcac atcgctgtgc tgctgacgta
    32041 gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt tctacttctt
    32101 tcaccatcgc aaacagcaca tcttccactt tgatctgcgt cgctttccag tcgttatgca
    32161 cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccgaa atggtcattt
    32221 ttcactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg cgatgttccc
    32281 ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgcacc ggcaatacaa
    32341 gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct ccctcatagt
    32401 catgctcata atttccgtta tcgggaacgg cgcggaggaa aaaacgctcc atgtcgtggc
    32461 gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt tgcagcaaca
    32521 gatgcagtaa accgatgtta acgcgcggca tatcagccag ctgattcaga atttcatccg
    32581 ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga taccacatgt
    32641 gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa ggataaaggt
    32701 gtgattaaaa aaacagcatt cgggagagta ttacgctctc ccgctctgtc agtattctga
    32761 attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt aggtagagtt
    32821 ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg taatatcgtt
    32881 atcggaatca ccataaaaaa ttcggatatt tttatcctgc agccattgcg attttgtatt
    32941 ttgccctggt ttatcgcccg caaagatcac cggattcatg ttggtggcag gaatatgaaa
    33001 attatccgcc agcgtttttg aaaccgtttc tgttttggtc gggctacgac cagtcacaaa
    33061 gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga cctcttttgg
    33121 aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat ttttcagata
    33181 atcttcgctt tctggcgaga aggttttttt gccgcgccag aagcccggac tggaaaaaag
    33241 taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga ggctattttc
    33301 aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg caacgttagt
    33361 accagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt ttagagcgaa
    33421 caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc ctaaatagtc
    33481 agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc ataacgccag
    33541 cagccgggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga tgattatatt
    33601 taacc
//
LOCUS       DAZKJR010000040        33396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKJR010000040 DAZKJR010000000
VERSION     DAZKJR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.040"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(446..2134)
                     /gene="betA"
                     /locus_tag="ACHOWS_001944"
     CDS             complement(446..2134)
                     /gene="betA"
                     /locus_tag="ACHOWS_001944"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFQ0047520.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKESVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GAECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGQEALPRSTARYFVANGMPVRAKKMSRDVN"
     gene            complement(2148..3620)
                     /gene="betB"
                     /locus_tag="ACHOWS_001945"
     CDS             complement(2148..3620)
                     /gene="betB"
                     /locus_tag="ACHOWS_001945"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFQ0047521.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3634..4221)
                     /gene="betI"
                     /locus_tag="ACHOWS_001946"
     CDS             complement(3634..4221)
                     /gene="betI"
                     /locus_tag="ACHOWS_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFQ0047522.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            4350..6383
                     /gene="betT"
                     /locus_tag="ACHOWS_001947"
     CDS             4350..6383
                     /gene="betT"
                     /locus_tag="ACHOWS_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFQ0047523.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            7260..8348
                     /gene="pdeL"
                     /locus_tag="ACHOWS_001948"
     CDS             7260..8348
                     /gene="pdeL"
                     /locus_tag="ACHOWS_001948"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFQ0047524.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(8390..9322)
                     /gene="yahB"
                     /locus_tag="ACHOWS_001949"
     CDS             complement(8390..9322)
                     /gene="yahB"
                     /locus_tag="ACHOWS_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0047525.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(9414..9911)
                     /gene="yahC"
                     /locus_tag="ACHOWS_001950"
     CDS             complement(9414..9911)
                     /gene="yahC"
                     /locus_tag="ACHOWS_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFQ0047526.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYFGMVWAFFAALAAGWLASVSGISGFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10169..10774
                     /gene="yahD"
                     /locus_tag="ACHOWS_001951"
     CDS             10169..10774
                     /gene="yahD"
                     /locus_tag="ACHOWS_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFQ0047527.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLNIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            10814..11677
                     /gene="yahE"
                     /locus_tag="ACHOWS_001952"
     CDS             10814..11677
                     /gene="yahE"
                     /locus_tag="ACHOWS_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFQ0047528.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQCGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            11667..13214
                     /gene="fdrA"
                     /locus_tag="ACHOWS_001953"
     CDS             11667..13214
                     /gene="fdrA"
                     /locus_tag="ACHOWS_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFQ0047529.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLRNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGMKQEHLDLHTLNQPLIADVRA
                     RMQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            13214..14632
                     /gene="yahG"
                     /locus_tag="ACHOWS_001954"
     CDS             13214..14632
                     /gene="yahG"
                     /locus_tag="ACHOWS_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFQ0047530.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPTAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            14658..14912
                     /locus_tag="ACHOWS_001955"
                     /pseudo
     CDS             14658..14912
                     /locus_tag="ACHOWS_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14962..15912
                     /gene="yahI"
                     /locus_tag="ACHOWS_001956"
     CDS             14962..15912
                     /gene="yahI"
                     /locus_tag="ACHOWS_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFQ0047531.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGVFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            15922..17304
                     /gene="yahJ"
                     /locus_tag="ACHOWS_001957"
     CDS             15922..17304
                     /gene="yahJ"
                     /locus_tag="ACHOWS_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0047532.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVQIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGNVAG"
     gene            17600..18010
                     /locus_tag="ACHOWS_001958"
     CDS             17600..18010
                     /locus_tag="ACHOWS_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0047533.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPLIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            18264..19247
                     /locus_tag="ACHOWS_001959"
     CDS             18264..19247
                     /locus_tag="ACHOWS_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0047534.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            19296..20780
                     /locus_tag="ACHOWS_001960"
     CDS             19296..20780
                     /locus_tag="ACHOWS_001960"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFQ0047535.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            20773..21744
                     /locus_tag="ACHOWS_001961"
     CDS             20773..21744
                     /locus_tag="ACHOWS_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0047536.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKMGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            21741..22697
                     /locus_tag="ACHOWS_001962"
     CDS             21741..22697
                     /locus_tag="ACHOWS_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0047537.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            22784..23833
                     /gene="yahK"
                     /locus_tag="ACHOWS_001963"
     CDS             22784..23833
                     /gene="yahK"
                     /locus_tag="ACHOWS_001963"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFQ0047538.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEHLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSELKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            24455..24700
                     /gene="yahM"
                     /locus_tag="ACHOWS_001964"
     CDS             24455..24700
                     /gene="yahM"
                     /locus_tag="ACHOWS_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFQ0047539.1"
                     /translation="MAVQLFKTLLNQIPLLSFLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(24717..25388)
                     /gene="yahN"
                     /locus_tag="ACHOWS_001965"
     CDS             complement(24717..25388)
                     /gene="yahN"
                     /locus_tag="ACHOWS_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFQ0047540.1"
                     /translation="MMKLLHLFMVEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            25535..25810
                     /gene="yahO"
                     /locus_tag="ACHOWS_001966"
     CDS             25535..25810
                     /gene="yahO"
                     /locus_tag="ACHOWS_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFQ0047541.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(25911..27497)
                     /gene="prpR"
                     /locus_tag="ACHOWS_001967"
     CDS             complement(25911..27497)
                     /gene="prpR"
                     /locus_tag="ACHOWS_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFQ0047542.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGHEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQESETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            27736..28626
                     /gene="prpB"
                     /locus_tag="ACHOWS_001968"
     CDS             27736..28626
                     /gene="prpB"
                     /locus_tag="ACHOWS_001968"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFQ0047543.1"
                     /translation="MYLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            28786..29955
                     /gene="prpC"
                     /locus_tag="ACHOWS_001969"
     CDS             28786..29955
                     /gene="prpC"
                     /locus_tag="ACHOWS_001969"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFQ0047544.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            29989..31440
                     /gene="prpD"
                     /locus_tag="ACHOWS_001970"
     CDS             29989..31440
                     /gene="prpD"
                     /locus_tag="ACHOWS_001970"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFQ0047545.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            31480..33366
                     /gene="prpE"
                     /locus_tag="ACHOWS_001971"
     CDS             31480..33366
                     /gene="prpE"
                     /locus_tag="ACHOWS_001971"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFQ0047546.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVATRIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         7963 a         8339 c         9041 g         8053 t
ORIGIN      
        1 aatcgctgct aataaattat acgaaccgtg tttaaagctc cacgtagttt ttggggctga
       61 cttattttta tactcgccac aagtcccctt ttcgtgcatg ataaccgaat aaattgactt
      121 ttttagcgta gacctgatat tactccacgc tttgggataa atacgcccgg cttacgcagt
      181 tgatcgctga ccactgctaa ccgggagtgg gaaagaacaa gtaaccctag ccaggagcac
      241 tggttgcgtg attcttaagc tgagtggcat tacacttgtg gtttatcagc gtatccatag
      301 caccatcatc cgccagattg agcgaatcgg cttaaagttt gatggcggga ctgcggatat
      361 gaagcatgag agttacctca gtgttttata taaggattcg gtcctcatag tagaacggta
      421 actctcaatc tgataggttc ctgagttagt tcacatcacg actcattttt ttcgctctca
      481 ccggcatccc atttgccaca aaatatcttg ccgtgctcct cggcaacgct tcttgcccgc
      541 gaatcatatc cgccattttc tcgccaatca taatcgtcgt agcgttcaaa ttgccggtga
      601 taatttgcgg cataattgac gcatccacca cgcgcaggcc ttccagcccg tgtacgcggc
      661 cttcggcgtc aaccactgcc atctcgtcat aacccatttt gcaggtaccg cacggatgaa
      721 acgcggtttc ggcgtggtta cgcacgaact catcgagctg ctcatccgtc tgacattccg
      781 cgccggggct gatttcgcgg ccacgatact gatccagcgc cggttgatgc atgatctccc
      841 gggtgatgcg aattgcgtcg cggaactcct gccagtcctg ctcgtgcgac atgtagttaa
      901 acagaatcgc tggatgctgg tgcgggtcgc gggatttaat ccgcacatgc ccacggcttg
      961 gcgagcgcat cgatccgacg tggcactgga agccgtgctc tttcactgcg ttcgagccgt
     1021 tatagttaat cgccactggc aggaaatggt actgaatatt cggccacgca aattcctcac
     1081 ggctgcgaat aaatccgcct gcttcaaagt ggttgctggc accaacgccg gtgccgccaa
     1141 acagccactc cgcaccgatt ttcggctgat tccaccactg cagggcaggg tagagggaaa
     1201 ccgattcttt gcactcatat tgcagataca tctccaggtg atcctgaaga ttttcgccga
     1261 cgccgggtaa atcatgcacc agcggaatat caaactccgc cagcagttca gcgttgccga
     1321 cgccggagcg ttgcaggatc tgcggtgaag caatcgcgcc tgcacataac agcacttctt
     1381 tattcgccgc cgcgcgggtc ggaatggtgc tgtcgccttc cagccactcg acgcccaccg
     1441 cgcgtttgcc gtcaaaaatg atgtgatcgg tcatggcgtg agtacgaatg gttaggttag
     1501 ggcgcgattt ggcctgatcg agatagccac gcgcggtgct ggcgcgacgg ccctgcggcg
     1561 tgacggtgcg atccatcggg ccaaaacctt cctgctgata gccgttgaga tcgtccgtgc
     1621 gcgggtagcc cgcctgcacg cccgcttcaa tcatcgcttc aaacagcgga ttgacgccgg
     1681 gtttggaggt ggtgacgctc accgggccat caccgccgtg gtagtcgttc tcgcccacat
     1741 cgcgagtctc ggccttgcga tagtagggca gacagtcgag gtagctccag ttctccagac
     1801 cgggttcttt cgcccagtta tcgagatcca gcgcgttacc acggatgtaa cacatgccgt
     1861 tgatcagtga tgacccgcca agccccttac cgcgtccgca ctccatgcga cggttgttca
     1921 taaacggttc cggttccgtc tcataggccc agttatagcg tttaccctgt aacgggaacg
     1981 ccagcgcggc gggcatctgg gtgcggaagt caaagcgata gtccgggccg cctgcttcaa
     2041 gcagcagcac ggttgtgttc ggatcttcag tcagacgggt agcgagaacg ttgccagctg
     2101 agccggcacc aataatgatg tagtcaaatt gcaaataaac ctcctggtta gaatatggac
     2161 tggaatttag ccatctcaac ctggatggac ttcacctggg tgtaactctg gagcgtcatc
     2221 acgccgttct cgcgaccaat gccggagtgt ttgtagccgc caacgggcat ctctgcaggg
     2281 gattcgcccc aggtgttgat ccagcaaata cccgcttcca gctgatgaat gacgcgatgc
     2341 gcgcggttca ggtccgccgt cacgatgccc gccgccaggc cgtagtcggt atcattggcg
     2401 cggcgaatca cttcgtcctc agattcataa gtgagaagcg acatcaccgg accgaagatc
     2461 tcttcgcgca cgatggtcat ttcgtcgcgg caatcggtga acacggtcgg tgcaacccat
     2521 gcgccgttat cgaagccatc gcctttcagt acatcgccgc cgcacagtac gcgcgcgcct
     2581 tcctctttgc ctttggcgat gtaacgcagc acgttatcgc gatgcgggaa gctgaccagc
     2641 gggccaaagt tagtttgcgg atcgaaaacg tcgcccgcgc gaatgcgctc aacgcgcgcc
     2701 agaatctttt gttcaaatgc ggctttgcat ttcgccggaa taaacacgcg ggtgccgttg
     2761 gtacacacct gaccggagct gaagaagttc gccatcatgg cgatatcagc ggcgagatcg
     2821 agatccgcat cgtcgaaaac gatcagcggt gatttaccgc ccagctccat ggtcacttct
     2881 ttcagggagg aagccgccga gttagccatc acttttttgc cgctggcgac accgccggta
     2941 aatgacactt tggcaatgcc cggatgctcg gtcagatact gcccggtttc cgcacctacg
     3001 cccggcagca cgttaaatac gccgtccggc aggcccgctt cgctgtaaat ttcggccagc
     3061 tttaacgcgg taagcggggt gacttcgctc ggtttgaaaa tcatcgcgtt gcctgccgcc
     3121 agcgccgggg cggatttcca caaggcaatc tggatcgggt agttccatgc gccaatccct
     3181 gccactacgc ccagcggttc gcggcgggta taaacaaatg acgtctcacg caatgggatc
     3241 tggctgcctt ccagcgacgg gatcagcccg gcgtagtact ccagcacgtc cgcaccggta
     3301 acgatatcga cggttgaggt ttccgaatac gcttttccgg tgtcgagggt ttccagtttt
     3361 gcgagttcgt cattgcgttc acgcagaata tcaacggccc gacgcagaat acgcgagcgc
     3421 tccatagcgg tcatcgccgc ccagattttt tgcccttgtt gggcactctt cacggcgcga
     3481 tcgacatcct cacgcccggc ggcctgcacg gtcgccagca cgttaccgtt ggccgggtta
     3541 atggtctcga aggtgcgacc gctggtggcg gaggtataac caccatgtat ataaagctgc
     3601 tgttctgcca ttcgggacat cacgtttcct cggttaatcg gtgggtaaat gctgagtaat
     3661 aaagtgacgg gtcagcgagt gtgcgagagg tttatccagc gctttgccgc tcagtgccgc
     3721 gcgcagccat aatccgtcaa tcagcgcggc aaggccgtag cccgcttcct gtgcctgttg
     3781 gcgcggtaac tcgcggcgaa attcgctcac cagattcgac aataagcggc ggctactgac
     3841 ctgctgcaag cgatagagca tcggctgatg catactgctg gcccagaagg ccagccaggc
     3901 tttcatcgcc gcactgctca cctgggtttc atcgaagttt ccaccaacaa tcgcctgtaa
     3961 tcgcagctct gcactgccct gcggaagtgc atgtaatcga ttcaaaaccg cgtcacgcag
     4021 ctgactggtg atatcgcgca tggttgcttc cagcagacca tttttgtccc tgaaatagtg
     4081 gctgatgatc cccgtagaaa cgcctgcacg gcgggcgatc tgcgcgatcg ttgcatcgtg
     4141 catgcccact tcatttattg cttccagcgt ggcgtcgatc agttgtctgc gccggatcga
     4201 ctgcatcccc aatttgggca ttttcgccac tccattcatc agcgttgttt atctattaaa
     4261 gcggttattg attggacgtt caatataaaa tgtgtcttaa ttgttacgaa tttgatttta
     4321 aatggtaaca ataacagtgg ggatactgga tgacagacct ttcacacagc agggaaaagg
     4381 acaaaatcaa tccggtggtg ttttacacct ccgccggact gattttgttg ttttccctga
     4441 caacgatcct gtttcgcgac ttctcggccc tgtggattgg ccgcacgctg gactgggttt
     4501 ctaaaacctt cggttggtac tatctgctgg cggcaacgct ctatattgtc tttgtggtct
     4561 gtatcgcttg ttcgcgtttt ggttcggtga agctcgggcc agaacaatcc aaaccggaat
     4621 tcagtctgct gagttgggcg gcgatgctgt ttgctgccgg gatcggtatc gacctgatgt
     4681 tcttctccgt agccgaaccg gtaacgcagt atatgcagcc gccggaaggc gcgggacaga
     4741 cgattgaggc cgcgcgtcag gcgatggtct ggacgctgtt tcactacggc ttaaccggct
     4801 ggtcgatgta tgcgctgatg ggcatggcgc tcggatactt tagttatcgt tataatttgc
     4861 cgctcaccat ccgctcggcg ctgtacccga tcttcggtaa acggattaac gggccgattg
     4921 gtcattcagt ggatattgca gcggtgatcg gcaccatctt cggtattgcc actacgctcg
     4981 gtatcggtgt ggtgcagctt aactatggct tgagcgtact gtttgatatt cccgattcga
     5041 tggcggcgaa agcggcactg atcgccttgt cggtgatcat cgccactatc tcggtgacat
     5101 ccggtgttga taagggcatt cgcgtgttat cggaactcaa cgttgcgctg gcgctgggat
     5161 tgatcctgtt cgtgttgttt atgggcgaca cctcgttcct gcttaatgca ctggtgctga
     5221 atgttggcga ctatgtgaat cgctttatgg gcatgacgct caacagtttt gccttcgacc
     5281 gtccggttga gtggatgaat aactggacgc tcttcttctg ggcatggtgg gtggcatggt
     5341 cgccgtttgt cggcttgttc ctggcgcgta tctcgcgtgg gcgtaccatt cgccagttcg
     5401 tgctgggcac gttgattatt ccgtttacct tcacgctgtt atggctctcg gtattcggca
     5461 atagcgcgct gtatgaaatc atccacggcg gcgcggcatt tgccgaggaa gcgatggtcc
     5521 atccggagcg cggcttctac agcctgctgg cgcaatatcc ggcgtttacc tttagcgcct
     5581 ccgtcgccac cattactggc ctgctgtttt atgtgacctc ggcggactcc ggtgcgctgg
     5641 tgctggggaa tttcacctcg cagcttaaag atatcaacag cgacgccccc ggctggctgc
     5701 gcgtcttctg gtcggtggcg attggcctgc tgacgctcgg catgctgatg accaacggga
     5761 tatccgcgct gcaaaacacc acggtaatca tggggctgcc gttcagcttt gtgattttct
     5821 tcgtgatggc ggggttgtat aaatctctga aggtagaaga ttaccgccgt gaaagtgcga
     5881 accgcgatac cgcgccgcga ccgctggggc ttcaggatcg cctgagctgg aaaaaacgtc
     5941 tctcgcgcct gatgaattat ccgggcacgc gttacactaa acagatgatg gagacggtct
     6001 gttacccggc aatggaagaa gtggcgcagg agctgcggtt gcgcggcgca tacgtggagc
     6061 taaaaagcct gccgccggaa gagggacaac agttggggca tctggatttg ttggtgcata
     6121 tgggcgaaga acaaaacttt gtctatcaga tttggccgca gcaatattcg gtgccgggct
     6181 ttacctaccg cgcacgtagc ggtaaatcga cctactaccg gctggaaacc ttcctgttag
     6241 aaggcagcca gggcaacgac ctgatggact acagcaaaga gcaggtgatc accgatattc
     6301 ttgaccagta cgagcggcac cttaacttta ttcatctcca tcgtgaagcg ccgggccata
     6361 gcgtgatgtt cccggacgcg tgattgttat tgcataaaac cgcgccatgt atgcatatgg
     6421 cgcggtttct cttacctctt gatacgttat atctgtacag ttaagccctt agtatagatt
     6481 gatgattgcc aggcaacaga taataaaaaa gaaaagaact attgaagccc aaaacctacg
     6541 tttggactgt tgtgaatgtt caataagctt agtcttaatt aatgtaaata ttggtgctaa
     6601 tttgaaatga cgctttattc atggggaacg cgtagcgcaa atcaacaaaa atcaatgtaa
     6661 ccatttgttg tttaaggttt aattgtctgt gtctgatatt ttattgattg ttttaaatat
     6721 tgtttttatt gtcattgtta taatgttggt catctattgc tgaatggatt cagtcttaag
     6781 aggcaggttg ttaagggata ggcatagagt aatgacgtgt atgcataacc aacatcttta
     6841 ctcattatgt cattgaatgt tgacgctatg tgtttatgag gttgaggtat tttcagttga
     6901 tctggattgt taaattcata taatgcgcct ttactcatga atgggcgtca atacgtagtg
     6961 ggaaattata aatatcgaaa tagtccaact acttctttat taccaaaaat gagtatctga
     7021 attttaatat tgcattcttg cgtgattatc tcctgagttt tgacttgtga ttaccttttt
     7081 aaggtattta gcgtaactgt ttctgagcga gcatcagagg taaagataat cttcttgata
     7141 gtgatgtggg atgttatacg tatggcatcg ctaatgttta tggttacccc ttatgtgtgc
     7201 tcaggaatcc acaggtaatc actcatactg aacagcgata aaagataaaa ggtgtgttca
     7261 tgaattcatg tgattttcgt gtttttctgc aagagttcgg tacaacggtt catttgtcat
     7321 tgcctggtag cgtatccgag aaagaacgac tgctactcaa gctgctgatg cagggaatgt
     7381 ctgtaacaga aatatcacag tacagaaatc gtagtgcaaa gaccatttca catcaaaaga
     7441 aacagctctt tgagaaactg gggattcaga gcgatattac tttctggcgg gatattttct
     7501 ttcagtacaa tccggagatc atatccgcca cgggtaataa tagtcacaaa tatattaatg
     7561 ataatcacta tcaccatatc gtcacgcctg aagccatcag tctggcgttg gaaaaccatg
     7621 aatttaaacc gtggatccaa ccggttttct gcgcgcagac tggggtactg acgggctgtg
     7681 aggtgcttgt ccgctgggaa catccacaaa cgggaattat cccaccggat cagtttattc
     7741 ctctggcgga gtcatccggt cttattgtca taatgacccg ccagttgatg aaacagactg
     7801 cggatattct gatgccggta aaacatttgc tgccggacaa tttccatatt ggcatcaacg
     7861 tctcggcggg ttgttttttg gctgcgggat ttgaaaaaga gtgtctgaac ctggttaaga
     7921 aattaggtaa cgataaaatc aaactggttc tcgagctaac ggaacgtaac cctattccgg
     7981 taacgccaga agccagagcg atatttgaca gccttcatca gcacaacatt acctttgcgc
     8041 tggatgactt tggtacgggt tatgcgacct atcgttactt gcaggcgttc ccggtcgatt
     8101 ttattaagat cgataagtca tttgtgcaaa tggcgagcgt ggacgaaata tccggtcata
     8161 ttgtggacaa tattgtcgaa ctggcgcgta agcctggtct gagtatcgtg gcggaagggg
     8221 tagaaaccca ggagcaggcg gatttaatga tcggcaaagg cgttcacttt ttgcagggct
     8281 atttgtactc tccgccagta ccgggtaata aatttatctc tgaatgggta atgaaagcag
     8341 gtggttgatg taaaccgcta ttcacagcgc atcgggaggt tggcagcgat taattctccc
     8401 gatgcagtaa atcctgatag atccccgtta atatgccatt tggcgaaaat tgttttttaa
     8461 tccactgcgt cacctgaccc gtagcagaat gctgagttgc gagtaacatg cgtgaatcct
     8521 ggcgcggatt atggatttgt cgggtaacca gcagggattg cgccatcgcc tcacggacca
     8581 tgtaatccgg caaaaaacca attccttcac ctaaaatctg gcactgacat ttggtgttga
     8641 aatctggcac cagaatcgac tcctgcccat gcagcaacca gcccactttt ttattaatcg
     8701 tatgcgcggt gtcctccacc atgatattgg ggtaaagacg cagctggctt tcggcgatcg
     8761 gctccggcac aaaggctaat gggtgatccg gggcgatagc aaaagcccag cgaattgcgc
     8821 caatctccgt gtaatcaatg ccgccgccgt ccagcagcgt atcgggtgcg ccgatagcaa
     8881 tattcgcctg attattaata atcgcatccc agacgccgtt atacacttcg gtcgtcacgg
     8941 taatctggca ggttggaaac tgctttttca gtacctgtaa taagcgtgcg gtgtgtttgg
     9001 gcgtataaag cagttggtta atacaaatgc gcacccgtgc ttctataccc tgcgaaatag
     9061 tatcgatgct gcgtttgatg gcgtgaaaat cattcagcag gtcggtagct ttgcggaaaa
     9121 aataacgccc ggactccgtt aactcaatgc ttcgggtact gcgagtgaac agcaccacat
     9181 ccagccctgt ttccattcgc ttgatggtgt agctaatggc ggaagtggtt aaacccaact
     9241 cttctgcggc tttactgaaa ctgccaaaac gcgcagcggt agtaaatgcc aacagattct
     9301 cttcggtaaa aattgagttc attaaaccat tcccgccatt atcgaatatg aatttctcca
     9361 gctatgactg tagaggtatc ggttaaagat agataatcat ttttgaataa cttttaatac
     9421 ccgtcgcgtt tagtcgtcgc ttcgcttaat ttttgtccgc catattctga aattatcccc
     9481 acgcagttac ccgccagaaa acccagtaat gtcaccgtcc agttcatccc gctggcaaaa
     9541 aatagcgtca tgccgagaaa gcctccggga ataaaagaca gtaaaccgaa acgtccttgc
     9601 cagaccacca ccgccgagaa gggtacagtc ataatcacgc tcgcccagaa cccggaaatc
     9661 ccacttacag aggccagcca gccggcagcc agcgcggcga aaaaggccca caccatcccg
     9721 aaataattaa ccgccagact tctgataaac cccgatttac cgccgcctgc ggcataaaaa
     9781 ctgcaaaagg cgacgaagcc aatggttcct aataattccc atccctgaga gagcccgaca
     9841 tgtgaagaca ctaactgcca aagccctgca caaataccga cggtgacacc cgttgccgtg
     9901 agtccgttca tcttgttccc ctctggttag acatggacga aagctactct tacatgtgca
     9961 gactatagag aaataaaacc tgattgagtg aaagggtatc gggtcaaaga aacaaatatt
    10021 gcactaccgc acactgcgaa aagattgttg aacatcattc aacaaaaagg cgaaatacgg
    10081 cgaattaaat ttaagcgggt tcttttatca gattatcgtt gctattctca attcatcgaa
    10141 aacagccaca aaaaccaggg gaatgtgaat gactataaaa aatctacctg ccgactattt
    10201 attggctgcg caacagggtg atatcgataa agtaaaaacc tgccttgcgc tgggtgtcga
    10261 tattaatacc tgcgatcgtc aggggaaaac ggcaattacg ctggcaagtt tatatcagca
    10321 atatgcttgc gttcaggcat taattgatgc cggagcggat attaataagc aagatcatac
    10381 ctgtttaaat ccttttttaa ttagctgtct gaacgatgat ttaacgctac tacgaattat
    10441 tttaccggct aaacccgatc ttaattgcgt cacccgtttt ggcggtgtcg gcctgacgcc
    10501 tgcctgtgaa aaaggccatt taaatattgt aaaagagctt ttggcgcata cggagattaa
    10561 cgttaaccag accaaccatg ttggctggac gccgctactg gaagcgattg tgcttaatga
    10621 tggcgggatt aaacagcagg cgattgtcca gttattgctg gaacacggtg ccagcccgca
    10681 catgacggat aaatatggca aaacgccgct ggaactggcg cgggaacgag gctttgaaga
    10741 gattgcgcag ttactgattg ccgcaggtgc ataaaccggg aggcttgcta tcaacacacc
    10801 agaaagacgg tgtgtgtggg cgctaactgc ggatgctgat tttctggcgc agcgggggca
    10861 aggacaggtt gaacaggtct ttgccagagc ggtaaatatc gcacttccgg cccgccagca
    10921 gttgctgacg ctgctttgtg aagagtacga caatgcgcca aacagttgtc ggttggcact
    10981 cactcacttt gatggtctgt tccggcatgg tgataaggtt cagtttgacg atcaaggcat
    11041 tacggttggt caacatcttc atatagagat gagtcgttgt cggcgttggc tgtccccaac
    11101 cttgcaaatg gccgctgtga attttcacct tatcgcctgg caacagtggc atgacattat
    11161 tcatcagcac ctgggggaaa atgaaaccct gtttaattat tgcggcgata atccgtttta
    11221 tcaggcgtta aataaagaat tacatattaa acgacgggca gttattcagg ccgtaaacga
    11281 taaacaaaat atcgcctcag cagtcgccag tatgatgggc ttagggattg gccttacgcc
    11341 atcagccgac gattatttaa caggtctggc gcttatttta tttatttccg gacatccggc
    11401 ggaaaaatac aaagaggaat tttatctcgg tctgcaatgc ggcagaaata ataccacgtt
    11461 attaagtgcc ataacgctgg aagccgcatt acaacaacgc tgccgggaaa atattcatcg
    11521 ttttattcat aacattattt atgacatccc tgggaacgca actcaggcaa tagaaaaaat
    11581 taaacatatt ggctccagtt ccggctgcga catgctgtat ggcatggccg atggttgtgc
    11641 gctgagccaa acctacggag ggaattatgt cagttaaaat agtcattaaa ccgaatacct
    11701 attttgattc tgtctcgctg atgtctatct ccacgcgtgc aaataaactc gacggcgtcg
    11761 agcaggcatt tgtggcgatg gcgaccgaaa tgaacaaagg cgtgctgagg aatttaggac
    11821 tgctgacgcc ggaactggag caggcgaaaa acggtgacct gatgattgtc atcaatggta
    11881 aatcgggtgc ggacaacgag cagttgctgg tggagattga agaactgttc aacagcaaag
    11941 cgcaaagtgg ctcgcacgag gcgcgttacg ccactattgc cagcgccaaa aagcatatcc
    12001 cggaaagtaa cctggcggtg atttcggtca acggtctgtt tgccgctcgc gaagcgcgtc
    12061 aggcgctgca aaacgatctt aacgtgatgc tgttttccga taacgtctca gttgaagatg
    12121 aactggcgct caaacagttg gcccacgaaa aagggctgct gatgatgggg ccagactgcg
    12181 gtacggcgat tatcaacggc gcggcgctct gctttggtaa cgccgtgcgt cgcggcaaca
    12241 tcggcattgt tggcgcatcc ggcaccggta gccaggaact gagcgtccgc attcatgaat
    12301 ttggcggcgg cgtttcgcaa ctgatcggca ccggcgggcg ggacctgagc gagaaaatcg
    12361 gcggcctgat gatgctcgac gctatcggga tgctggaaaa cgatccgcaa actgaaatca
    12421 ttgtgcttat ctccaaaccg ccagcgcctg cggtggcccg caaagtgctg gaacgcgcac
    12481 gcgcctgccg taagccagtg gtagtctgct tcctcggtcg tggcgaaacg ccagtggatg
    12541 agcaggggct acagtttgct cgcggcacca aagaagcggc actaaaagcg gtgatgctct
    12601 ccggtatgaa acaagaacat ctcgacttgc ataccctgaa ccagccgttg attgcggatg
    12661 tgcgtgcgcg tatgcaaccg cagcagaaat acattcgtgg cctgttctgc ggcggcacgc
    12721 tgtgtgacga aaccatgttt gcggtgatgg aaaaacatgg cgatgtctac agcaacattc
    12781 agcccgatcc tgaattccgc ctgcaagata tcaaccgcag cgtcaaacat accttcctcg
    12841 actttggcga tgacgatttt accaacggca agccgcatcc aatgattgac ccgaccaacc
    12901 gcatcagccg gttgatcgaa gaagcgcgcg atccagaagt ggcggtgatc gtgatggatt
    12961 ttgtgctcgg gtttggatcg catgaagatc cggtcggttc caccatcgag gcgatcaaag
    13021 aggcgaaagc gatcgccgct gccgaagggc gcgagttgat cattctcgcc tatgtgctgg
    13081 gtaccgatct cgatacgcca tcgttagaac aacaaagcca gatgttgctt gatgccggag
    13141 tgattctggc cagcagcagc accaataccg gattgctggc gcgtgaattt atctgcaaag
    13201 gggaggaagc ctgatgagcc cgtcactgtt tagccaaccg ttgaacgtta ttaacgtcgg
    13261 tatcgccatg tttagcgatg atctgaaaaa gcagcatgta gaagtgaccc aactcgactg
    13321 gacgccgccg gggcagggca atatgcaggt ggtgcaggcg ctggacaaca ttgccgattc
    13381 cccgctggcg gacaaaatcg ccgccgctaa ccagcaggcg ctggagcgca ttatccagtc
    13441 gcatccggtg ctgattggtt ttgatcaggc gattaacgtg gtgccgggta tgacgccgaa
    13501 aaccattctt cacgccgggc cgccgatcac ctgggaaaaa atgtgtggcg cgatgaaagg
    13561 cgcggtcacc ggagcgctgg tgttcgaagg gctggcgaaa gatctcgatg aggcggctga
    13621 actggctgcc tccggggaga tcaccttctc gccgtgccac gagcacgact gcgtgggttc
    13681 gatggcgggt gttacctcgg cctcgatgtt tatgcacatc gtaaaaaaca aaacctacgg
    13741 caacatcgct tacaccaata tgagcgagca gatggcgaag attttgcgta tgggcgctaa
    13801 cgaccagagc gtgattgacc gcctcaactg gatgcgcgat gtgcaggggc ccatactgcg
    13861 cgacgcgatg aaaattatcg gcgaaatcga tctgcgctta atgctggcgc aagcgctgca
    13921 tatgggcgat gagtgccata accgcaataa cgccgggacg acgctgctga ttcaggcgct
    13981 gacgccaggg attattcagg cgggctattc cgtcgaacaa cagcgggaag tgtttgagtt
    14041 tgtcgccagc agcgactact tctccggccc gacgtggatg gcgatgtgta aagcggcgat
    14101 ggatgcggcg cacggcattg aatacagcac tgtggtcacc accatggcgc gtaacggcgt
    14161 cgagttcggc ctgcgggtca gcggcctgcc ggggcaatgg tttaccggcc cggcgcagca
    14221 ggtgatcggc ccgatgtttg ccggttataa gccggaagat tcggggctgg atatcggcga
    14281 cagcgccatc actgaaacct acggcattgg cggatttgcc atggcgaccg cgcctgcaat
    14341 cgttgcgctg gtgggcggca cggtggaaga ggctatcgat ttctcccgcc agatgcgtga
    14401 aatcactctc ggtgaaaacc ccaacgtcac cattccgctg ctcggtttta tgggggtgcc
    14461 gacggcaatc gacatcaccc gcgtgggcag tagcggcatt ctgccggtga tcaacaccgc
    14521 catcgcccat aaagatgcgg gcgtcggcat gattggcgcg ggcattgtgc atccaccatt
    14581 cgcctgcttc gagaaagcca ttcttggctg gtgcgaacgt tacggcgtct gagttacatg
    14641 tttatgccgg atgcgacatg aatgtcttat ccggcctacg aatggcgcgg aaatctgtag
    14701 gcctgataag cgtggcgctt caggcattgg ccaccacagc cggatgcgac gtgaacgcct
    14761 tatccggcct acgaatggca cggaaatctg taggcctgat aagcgtagcg catcaggcat
    14821 tggtcaccac agccggatgc ggcgtgaacg ccttatccgg cctacgagtg gcgcaagaat
    14881 ctgtaggcct gataagcgta gcgcatcagg cagtctggcg ttggtcataa ccccatcacc
    14941 ctctgtagcg gacataaaaa tatgaaagaa cttgtggtcg ttgccattgg tggcaacagc
    15001 attatcaaag ataacgccag ccagtcgatt gagcatcagg cggaggcggt gaaagccgtc
    15061 gccgatacgg tgctggaaat gctggcttcc gattacgaca ttgtgctgac ccacggtaac
    15121 gggccgcagg tcgggctgga tttacgccgc gcggagattg ctcacgagcg cgaagggctg
    15181 cccttaacgc cgctggcaaa ctgtgtggcg gatacgcaag gtggcattgg ctatctgatc
    15241 cagcaggcgc tgaacaaccg gctggcccgc cacggcgaga aaaaagccgt caccgtggtg
    15301 actcaggtgg aagtggataa aaacgatccg ggatttgccc atcccaccaa gcctatcggc
    15361 gtattcttca gtgaaagcca gcgtgacgaa ttacaaaagg caaaccctga ctggcgtttt
    15421 gttgaagatg ccggacgggg ctatcgccgc gtggtcgcct cgccggaacc gcaacgtatt
    15481 gtagaagcac ctgccattaa ggccctgatc caacaaggct ttgtggtgat tggcgcgggc
    15541 ggcggcggaa ttcctgtggt gtgcagtgaa gcgggagatt accaaagcgt ggacgcggtt
    15601 atcgacaaag atctctctac cgcgctattg gcccgtgaaa ttcacgccga cattctggta
    15661 attaccaccg gcgtcgaaaa agtgtgtatt cactttggca aaccgcagca gcaggcgctg
    15721 gatcgggtgg atattgccac catgacccgc tatatgcagg aagggcattt cccacccggc
    15781 agcatgttgc caaaaatcat cgccagcctg acgttcctgg agcagggcgg taaagaagtg
    15841 attatcacca cgccggaatg cctgcctgcg gcgctgcgtg gtgaaacggg cacccatatt
    15901 attaaaacgt aaggacgtaa gatgaaagaa aacaatagcc gccgtgagtt tctgagccag
    15961 agcggtaaga tggtcaccgc cgccgcgctg tttggtacct ctgtgccgct cgcccatgcg
    16021 gcagtctctg gcacaatcca ttgcgacgcg aataacacca tgaaaatcac tgacccgcac
    16081 tactatctcg acaacgtgtt gctggaaacc ggttttgact acgaaaatgg cgtggcggtg
    16141 cagacccgca cggcgcgcca gactgtggaa attcagaacg gcaaaattgt tgccctgcgc
    16201 gagaacaatc agcatccgga cgccacgctg ccgcactatg acgctggcgg taagctgatg
    16261 ctgcccacca cccgcgacat gcatattcat ctcgacaaaa ccttctatgg cgggccgtgg
    16321 cgctcgctca atcgtccggc aggcaccacc atccaggaca tgatcaaact cgagcagaaa
    16381 atgctgccgg agttgcaacc gtacactcag gaaagggcgg aaaaactgat tgatttattg
    16441 cagtcgaaag gcactaccat tgcccgcagc cactgcaata tcgaaccggt ttccggcctg
    16501 aaaaatctgc aaaatttgca ggcggttctg gcgcgacgtc aggcgggctt cgagtgtgaa
    16561 attgtcgcct tcccgcagca cggtttgctg ctgtcgaaat cagaaccctt aatgcgcgaa
    16621 gcgatgcagg cgggggcgca ttacgtcggc gggctggacc cgaccagtgt tgatggcgcg
    16681 atggaaaaat ccctcgacac catgttccag attgcgctgg actacgacaa aggcgtcgat
    16741 attcacctgc acgaaaccac tccggcaggc gtggcagcca tcaattatat ggttgaaacg
    16801 gtagagaaaa cgccacagct gaagggcaag ctgaccatca gccacgcctt cgcgttggca
    16861 acgctcaacg aacaacaggt agatgaactg gcgaaccgga tggcggcgca gcaaatttct
    16921 atcgcctcga cggtgcagat cggcacgcta catatgccgc ttaaacaact gcacgacaaa
    16981 ggcgtaaaag tcatgaccgg aactgacagc gttattgacc actggtcgcc ttacggcctg
    17041 ggcgacatgc tggaaaaagc caatctctac gcgcagctct atattcgtcc taacgaacag
    17101 aacctctccc gttcgctgtt tttagctact ggcgatatat tgccgctcaa cgaaaaaggc
    17161 gagcgtgtgt ggcctaaagc tcaggatgac gccagctttg tgctggtgga cgcctcctgt
    17221 tccgccgagg cggtggcgcg tatctcgccg agaaccgcaa cgttccataa agggcaactg
    17281 gtgtggggga atgtggcagg ttgatgcgca tttgatatgt ctgcaacggt gaacagtaag
    17341 agatttaagc cccagggagt ggggcgggaa tggtttgtta atgaatgatc ctgaacttta
    17401 taatctcccg gcataatttc caggggatta tttgagagaa atacacaaaa gtgagtttta
    17461 acaaaggtat ttatcaagcc ataaatgtga cctgcttcgg ataaaaaaga ttaaacatag
    17521 gaaaaccaat gatactcttg ctgatggtta ccaacagtaa ttaagatgtg attttttccg
    17581 taatcgaaaa aatattgcca tgaccataaa agaaaaattc tcacaaaaat atccccatgc
    17641 ttccttttgt acttttggcg attcagcagc gttggccgat cacctcgcaa cgttgattgc
    17701 gactggtgtt aaaacagctt cctgtggttc gctggcggga tgtattgaag acaacgcatt
    17761 tccgctgatt ggtgagtata agattgtaga aaatagccgt ggtgagcctg tctgtgttat
    17821 cagagtgatt ggcctgcatt tactgcgctt ttctgatgtc accgcagagc ttgcccgtaa
    17881 ggaaggtgaa ggtgacctca gtcttgaata ttggcgtaat gaacaccgtc gttttttcca
    17941 ggcagaagga tgctattcac cagaaatgga cgttatcttt gaagaatatg cccttattga
    18001 cgttgtgtag ataactattt ttctacgcga aataagctca caaacataaa gtatcgctaa
    18061 ttttgtttat tttgctgttt tttacgtgat gattgtcaca cttattgttg ctgcccgttt
    18121 tgtccatatt tataaccaaa tttttaatag ctgcctgaaa tagggctgtt tattctgcgt
    18181 aaaaatattc gaaaattctt attctctcca gcgcgctaat ttgggcgaac acttcctgac
    18241 taccctgcaa tgaggctgaa atgatgaata aacgttttgt tatcaatatg gtgtcttcgc
    18301 tgttattagg cgctgcgcta atttctgcgc cgctccaggc agcggaaaaa gtcgtggtga
    18361 atatctctaa agtcgatggt atgccgtggt ttaatcgcat gggcgaaggt gtggttgagg
    18421 cgggtaaagc ctttggtgtt aatgcgtcac aggtggggcc ttcaagtact gatgctccgc
    18481 aacaggttaa aattattgag gatttaatcg cccgtaaggt taatgcaatt actatcgtgc
    18541 caaacgatgc caacgtgctg gaaccggtat tcaaaaaagc gcgagatgcc ggaattgtgg
    18601 ttctgaccaa cgaatctccc ggtcagccaa gtgctaactg ggatatcgaa atcattgata
    18661 acgaaaaatt tgccgctgaa tatgttgagc atatggcgaa acgcatgggg ggcaagggcg
    18721 gctatgtgat ttacgtgggt agtttgaccg tgccgcagca taacttatgg gctgacttgc
    18781 tggtgaaata ccagaaagag cattacccgg atatgcatga agttacccgc cgcatgccgg
    18841 ttgccgagag tgtggatgac tcacgtcgta ccacactgga cctgatgaaa acctatcctg
    18901 acttgaaagc ggttgtctcc tttggttcaa acggtccgat tggcgcggga cgtgcagtga
    18961 aagagaaacg ggcgaaaaat aaagtcgcag tctatggcat gatgatccct tcacaggcgg
    19021 catcactgat taaaagtggt gatatcaccg aagggattac ttacgatccg gcctctgcag
    19081 gttacgcact cgcggccgtc gccagcacgt tgctgaaggg tgaagagatc aaacctggat
    19141 tagaaatgca gaatctcggt aaggccgacg tagatatgga taagcgaatt atccgtttcc
    19201 ataaggtatt actggtcaac aaagacaata tcgactcact ttattaatcc atttcttcgg
    19261 ggcgaaagcc ccgattctta ttgccagggg atgctatgga aaccttcctt tcccttcgtc
    19321 atatcaacaa aacgtttcat gccacgcgag cgctgcgcga tgtttctctc gactttatgt
    19381 cgggtgaggt gcactgcctt gccgggcaaa acggctgtgg taaatcgacg ttgattaaaa
    19441 ttatgtccgg tgtttatcgc ccggatgagg gggcggaaat tacgcttggt gggaaaaact
    19501 ggtcaaagct gacacccgcc gcttcggtgg cgcaggggat tcaggtgatt tatcaggacc
    19561 tctctttatt tcctaacctg agtgtctggg agaatatcgc cgtgaatcac tatcaccacg
    19621 gtctgtttgt taaccgccgt cgtctgcgtg aggtggcgca ggcggcaatg accagtatta
    19681 acgtcacatt gccactggat acgctcgttt ctgagctttc aattgcccgc tgtcagctgg
    19741 tggcaatttg ccgtgcactg gcgcaggacg cacggttgat cgtgatggat gaaccaaccg
    19801 cttcgctgac ccatcaggaa gtgcaagggt tattacaggt ggtgcatcaa ttgcgggaac
    19861 gcgggatctg cgtggtcttc gtcagtcatc gtctggaaga agtgatggaa gtttccgacc
    19921 gtatttcagt gctgaaagat ggtgagctgg tcgggacctt tccggccgca gagatgacca
    19981 caaaacaact cagttttctg atgaccggcc aggagttcga atatcaggta cgagagttgt
    20041 ggcagggaaa atccagcacc ccggtgctgg aggtgcgtaa tttaagtcgt catggggaat
    20101 atcaaaatat taacttgcgg gtggaagcgg gcgaagtggt gtcgattgtt ggcctgcttg
    20161 gtgcggggcg cacggaatta tgtctgagtc tgtttggtat gacccgaccg gatgctggtg
    20221 agatcctcat caacggtcag ccggttacac tgcatagtaa ccaggacgcc atccgccacg
    20281 gtattggtta tgtttcggaa gatcgcatgt cgcgcggttt ggtgatggcg caatctattg
    20341 aagacaacat cattagcact gtttttcaca aggtaaaaga tcgcttcggc tttctgagtg
    20401 aagctaaagt gcgtgacctg gtggacaggc taatcaaggc attgaccatc aaagtctctg
    20461 acccacatct cccggtgaat acgctttccg ggggtaacgc gcagcgagta tcgatcgcca
    20521 aatggctggc gattgggcca cgactgttga ttctcgatag cccgactgtc ggtgttgata
    20581 tcgccaacaa agcaggaata tacggcatta tcagcgatct ggcggctcat ggcattgcgg
    20641 tattgatgat ctgcgatgaa attgaagaag cgtggtatca aagccaccgg attttggtaa
    20701 tgcaaaaagg tcagatcacc catagcttcc tgcctgacag cagttctcag gccaggattg
    20761 cggaggtggt aaatggctga actgaaaaaa cgccatgaat tctggctggc attactgatt
    20821 gtggtgctgt ttgttggcct tgcctggcgc agcgacgagt ttctgacatt cggtaatttg
    20881 tacgatctcg ccaataacta tgccatgttg actattctcg cctgtggttt gtttgtggtg
    20941 ttaatttccg gtgggattga tatttcgttt ccggcaatga ccatcattgc gcaatacggc
    21001 atggtgctgt tgctgcaaaa aatgggtggc aactttgctg tcgcgtttgc cctggcgggc
    21061 ggcatcggca ttttacttgg cttaataaac gccttactgg ttaatcgcct acgggtgccg
    21121 tctatcatca tcactatctc gacgctgaat attttttatg gcctgctgtt atggttgagt
    21181 aaaggtgtgt ggctgtacga ctttccgccg tggtttgagc agggggtaat gttgttcaag
    21241 tacaccgatg ctgatggcta tgactatggc cttggtctgc cgctgatcgc catgattacg
    21301 gtggtgctgc tgacagcgtt tatcatgaat ttcaccagtg tagggcgcaa aatttatgcc
    21361 cttggcggga accgcgaatc agccagtcgc atcggcttta gtgtgctgaa actgcaactt
    21421 ttcgtctatg gctatatggg attgatgtct ggtgctgcgg gtgtagtgca gtcgtggacg
    21481 gtgatgactg tcgcccccga ttctcttctg ggttatgagc tgacagtact ggctgcggtg
    21541 gtgcttggtg gcactagttt gctcggcggg cgcggcacgt taaccggtac tttgctcggc
    21601 gtggtgttgt tggcagtaat gcaaaacggg ctaaatttat tgggagtctc gtcttactgg
    21661 caaacattga tcacaggcat catcatcgtt gccagcatta gcgccacggc gtggagtcag
    21721 catcagaacc ggagtctgct atgaaaaaat cgtggcgtaa taacgttgag ttttacttga
    21781 tcggcctgct ggtgctaact gttgccgcgt tcagcattac tatgccggag attttctggt
    21841 cgataagtaa tttccagtcc gtcgcttcgc aaatgcccgt gctgggcatt ctggcgttgg
    21901 ctatggcggt gaccatgctt tgcggtggca ttaacttgtc aattatagcc actgctaacg
    21961 cctgttcact ggtgatggcg tgggtggcga ctcagtatcc accgggtatt gctacggttg
    22021 tcgctacgct gctggcgggc gctggcgcgg cggtgataat cggtttatgt aacggcgtat
    22081 tgattgctgg tattcgagtg tcgccaattc ttgcaacgct tgggatgatg acgttgctca
    22141 aaggggtaaa tattctggtg acaggcggta gcgccattgc caattatccc tcatgggtgc
    22201 tgtggttgaa tcatgcacag tggttcggta ttccgctacc gatgtggttg tttaccgcag
    22261 tggcgctggg gctgtggata ttgctggaga aaacgccgct cggtaaatcc atttatctga
    22321 ttggttctaa cgaacgagca acactttact ccggtattaa cactcgtcgg gtgctgatat
    22381 gggtgtatgt catttccgcc ttgctttgtg cggtagcggc atttttgatg atgtcgaaac
    22441 tcaactcggc taaggcctct tatggcgaat cttacttgct agtgtcaatc ctcgcggcag
    22501 tactcggcgg ggttaatccg gacggcggca gcgggcggat aattggtatg gtgctggcgc
    22561 tgtttcttct gcaaattatt gaaagcggct tcaatattct cggtataagt ccctacctga
    22621 cgatggcctt atggggaacg ttgctgctct gtttcattca ggctcgcggc atgttggggc
    22681 tggatcgggt ggtttaaata ttggctatat tcaatggacg cgttttgccg cgatgacata
    22741 tcaggcatcg ccaaatacac atagctaatc aggagtaaac acaatgaaga tcaaagctgt
    22801 tggtgcatat tcagctaaac aaccacttga accgatggat atcacccggc gtgaaccggg
    22861 accgcatgat gtcaaaattg aaatcgctta ctgtggcgtc tgccattccg atatccacca
    22921 ggtccgttcc gagtgggcgg ggacggttta tccctgcgtg ccgggccatg aaattgtagg
    22981 acgcgtggta gctgttggtg atcaggtaga aaaatatgcg ccgggcgatc tggttggtgt
    23041 cggctgcatt gtcgacagtt gtaaacattg cgaagagtgt gaagacgggc tggaaaacta
    23101 ctgtgatcac atgaccggca cctataactc gccgacgccg gacgaaccgg gccatactct
    23161 gggcggctac tcacaacaga tcgtcgttca tgagcgatat gttctgcgta ttcgtcaccc
    23221 gcaagagcac ctggcggcgg tggctccttt gttgtgtgca gggatcacca catactcgcc
    23281 gctacgtcac tggcaggccg ggccgggtaa aaaagtgggc gtggtcggca tcggcggtct
    23341 gggacatatg gggatcaagc tggcccacgc gatgggggcg catgtggtgg catttaccac
    23401 ttctgagtta aaacgcgaag cggcaaaagc cctgggggcc gatgaagttg ttaactcacg
    23461 caatgccgat gagatggcgg ctcatctgaa gagtttcgat ttcattttga atacagtagc
    23521 tgcgccacat aatctcgacg attttaccac cttgctgaag cgcgatggca ccatgacgtt
    23581 ggttggtgcg cctgcgacac cgcataaatc gccggaagtt ttcaacctga tcatgaaacg
    23641 ccgcgcgata gccggctcta tgattggcgg cattccagaa acacaggaga tgctcgattt
    23701 ttgcgccgaa catggcatcg tggctgatat agagatgatt cgggccgatc aaattaatga
    23761 agcctatgag cgaatgctgc gtggtgatgt gaaatatcgt tttgttatcg ataatcgcac
    23821 actaacagac tgaaaaaatt aataaacatc ctgtggttta acatattaac ttcgctccac
    23881 ttaacttttt agttaaggag agcgaaataa tatcaaagta gtggtaaacc tatagcgtaa
    23941 attcagattg ttaaattaac tccactactt tacacgttag ttatgagcga tttctgatag
    24001 tgtctggttt aatcagagct ttattatctg cgacgtttat ttttatttaa tagagccacc
    24061 tgcaagttgt tattccacca gagagtgaat cgcaaagcag gtaatcattt catctgtata
    24121 tctgtatgcc aactggcatt ccatctcttt tttcgccgat attttgttgt gatcggcgac
    24181 acttcgctta aaaaagcatc agcagtggtt tcgcagctat gcggtgtata aaaaatgatc
    24241 tcatgcagat gttttgtgaa tgtgtgggtt gacattcata tgaaaaaaat cataattcca
    24301 tcatgtttgt gtggggtctt ttctgtatct tacgcatcgc actcaagcct gacagaaaat
    24361 atgctgtaag gctcatatca aaaaccgcca ttagctcatc aggaagagca gacgacaacc
    24421 aggattgttg tatggtacgg ggttcgaggc ctcgatggca gtccagcttt tcaaaactct
    24481 tctgaatcag atccccttat tatcattcct gcaaagtgga acattgccat tgtttggcta
    24541 ttcaggacgg gtcagaccaa tgaaaaaagc ccacacaggg gagagtgggc tgaaatggga
    24601 agctaaagac tcaagtaaac ttatcggaaa taaggaccac gcattacggg ggctatcatc
    24661 gccgatggtc gggattcggc aaatcaggtt gattacgtag agagtaaatt atctgctcac
    24721 cgctgcgtca ccccttcgta aatcaggcgt agcgcgaata caccaataat tgcaccaata
    24781 acccgactgg caacgcgttg catacgccca taagcacgac gcactgcggg caaagaaaac
    24841 gcctgactaa gaaaaacgcg ccagataact gatgcgagca caatccccgc ccaggccatt
    24901 aaacgtgccc atgttggtgt ttcggcattt aatgttactg agaaaatact aataaaaaat
    24961 aaaacggttt gcgggttaga gagatcggta attaatccac ggcggaaaaa gatataccag
    25021 ggggcactaa tcggttgttg tagtgtgctc atttgcggtg ttgactggcg gcgcatgctg
    25081 caccacgcaa accataagag ataagcgccg ccgacgattc tgataagcga aaaaatctcc
    25141 tcacactgcg taattagtgt tgcaagacca aacaaaccca acccggaata aaatgcatcg
    25201 cccaacgcca cgcccagtcc ggtcagcacc cctgcgcgtc gaccggaagc caggctggtt
    25261 tgtactacca caaagagatt ggctcccgga ttaaaaaaag taatcacgaa cagtcctacg
    25321 gtcagataaa cggcgtgcaa aggatccata gtgatttcaa ccataaataa gtgaagtaac
    25381 ttcatcatat ttctaccaaa aataatcggg tgcgagagag atcacaaagt gtcttatttc
    25441 cggttactgg cgtttatgcc ctgactgaac taattattaa tcaacccaat aatgtgggtg
    25501 ggtgatagtg tgataacaac tctggagccg taatatgaaa ataatctcta aaatgttagt
    25561 cggtgcgtta gcgtttgccg ttaccaatgt ctatgccgca gaattaatga ccaaagcgga
    25621 atttgaaaag gttgcatcgc aatatgaaaa aataggtgat atttcaacca gtaatgaaat
    25681 gtcgaccgca gatgcgaaag aagatttgat caaaaaagcc gatgaaaaag gggctgatgt
    25741 gttggtactg acctctggtc aaactgacaa taagatccac ggcacggcaa atatttataa
    25801 gaagaagtaa ttctgaatcc tatgtaaaca tccccgatgc gtaagtttat cggtgatcat
    25861 catctattga aatttatgcc ggataaagcg ttcgcgctgc attcggcagt tcagcttttc
    25921 agccgccgcc agaacgtcgt ccggctgata cctaaataat tcgccgctgc tgttttatcg
    25981 ccattaaatt tctccagtgc ctgttgtgct gtcagcaagc ctggaatggg agtcttcgcc
    26041 gactcgcgtg ccagttccgg cagtagcagc tgcaaaaatt gcggcgttaa atccggcgtc
    26101 gattccacac ttaaaaacag cgccagtcgt tccatcatat tgcgcagttc acgaatattg
    26161 cccggccagt cgtagtgcac cagcatggtt tcgctttcct gtaatccctg gcgtaatgcg
    26221 gcagcaaacg gggcggagag cgccgccaga gacactttca aaaaactttc cgccagcggc
    26281 agaatatccg ccacccgctc gcgcagtggt ggcaattgca gacgcaaaat actcagccga
    26341 taaaacagat cacggcgaaa ctgcccttgc cgcatatctt cttccagatt gcagtgagtg
    26401 gcgctaatga ctcgcacatc caccggaaca ggctgatgcc cgccgacgcg ggtgacctct
    26461 ttttcttcca gcacccgcag cagccgagtc tgcaacggca gcggcatttc gcctatctcg
    26521 tcaagaaaca gcgtgccacc gtgggcgatt tcgaacagcc cggcgcgacc tccgcgtcgc
    26581 gagccggtaa acgccccttc ctcatggcca aacagctccg cttccagcag cgattcggca
    26641 atcgctccgc agttgacggc aacaaacgga tgcgactttt tgccctgtcg cgcatcgtgg
    26701 cgggcaaaat attcccgatg aatcgcctgg gccgccagct ctttgcccgt ccccgtttcc
    26761 ccctcaatca acaccgccgc actggagcgg gcatacagca aaatagtctg ccgcacctgt
    26821 tccatctgtg gtgattgacc gagcatatcg cccagcacgt aacgagtacg cagggcgttg
    26881 cgggtggcat cgtgagtgtt atggcgtaac gacatgcgcg tcatatccag cgcatcgctg
    26941 aatgcctggc gcacggtggc ggcggaatag ataaaaattc cggtcattcc ggcttcttct
    27001 gccagatcgg taatcagccc cgcgccgacc accgcttcgg tgccgttagc tttcagctcg
    27061 ttaatctgcc cgcgcgcgtc ttcttcggta atgtagctac gttgatcgag gcgcaaatta
    27121 aaggtttttt gaaacgccac cagtgccgga atagtttcct gataagtgac aacgccgata
    27181 gaagaggtga gctttccggc ttttgccaga gcctgtaaca catcgtagcc gctcggttta
    27241 atcaaaataa ccggcactga caggcgactt ttaagatatg cgccgttaga gccagcagcg
    27301 atgatggcgt cgcagcgttc gtttgccagt tttttgcgga tgtaggtcac cgctttttca
    27361 aagccaagct gaataggggt gatattcgcc aggtgatcaa actcgagact gatatcgcga
    27421 aacagctcga acagccgcgt tacagatacc gtccagatca ccggtttgtc gtcattcagc
    27481 cgtggtggat gtgccatagc gcaccgcaaa gttaagaaac cgaatattgg gtttagtctt
    27541 gtttcataat tgttgcaatg aaacgcggtg aaacattgcc tgaaacgtta actgaaacgc
    27601 atatttgcga attacttcat gactatattg ctaacaaatt gaaattaaac atttaatttt
    27661 attcagtcaa ttgtggcaca ccccttgctt tgtgtttatc aacgcaaata acaagttgat
    27721 aacaaaggat gggctatgta tctacactct ccaggtaaag cgtttcgcgc tgcactgtct
    27781 aaagaaaatc cattgcagat tgttggcacc atcaacgcta accatgcgct gctggcgcag
    27841 cgtgccggat atcaggcaat ttatctctcc ggcggtggcg tggcggcagg gtcgctgggg
    27901 ctgcccgatc tcggtatttc tactcttgat gacgtgctga cagatattcg ccgtatcacc
    27961 gacgtttgtt cgctgccgct gctggtggat gcggatatcg gttttggttc ttctgctttt
    28021 aacgtggcgc gcaccgtgaa atcgatgatt aaagccggtg cggcaggatt gcatattgaa
    28081 gatcaggttg gtgcgaaacg ctgcggtcat cgtccgaata aagcgatcgt ctcgaaagaa
    28141 gagatggtgg atcggatccg cgcggcggtg gatgcgaaaa ccgatcctga ttttgtgatc
    28201 atggcgcgca ccgatgcgct ggcggtagag gggctggatg cggcgattga gcgtgcgcag
    28261 gcctatgttg aagcgggtgc cgagatgttg ttcccggagg caattaccga actcgccatg
    28321 taccgccagt ttgccgatgc ggtgcaggtg ccgatcctcg ccaacatcac cgaatttggc
    28381 gcaacaccgc tgtttaccac cgacgaatta cgcagcgccc atgtcgcaat ggcgctgtac
    28441 ccactttcag cgttccgcgc catgaaccgc gccgctgagc atgtctacaa cgtcctgcgt
    28501 caggaaggga cgcagaaaag cgtcatcgac accatgcaga cccgcaacga gctgtacgaa
    28561 agcatcaact actaccagta cgaagagaag ctcgacgacc tgtttgcccg tggtcaggtg
    28621 aaataaaaac gcccgttggt tgtattcgac aaccgatgcc tgatgcgccg ctgacgcgac
    28681 ttatcaggcc tacgaggtga actgaactgt aggtcggata agacgcatag cgtcgcatcc
    28741 gacaacaatc tcgaccctac aaatgataac aatgacgagg ataatatgag cgacacaacg
    28801 atcctgcaaa acagtaccca tgtcattaaa ccgaaaaaat cggtggcgct ttctggcgtt
    28861 ccggcgggca atacggcgct ctgcaccgtg ggtaaaagcg gcaatgattt gcattaccgc
    28921 ggctacgaca ttcttgatct ggcggaacat tgcgaatttg aagaagtggc gcatctgctg
    28981 atccacggca aactgccgac ccgtgacgaa ctcgccgcct acaaaacgaa actgaaagcc
    29041 ctgcgcggtt taccggctaa cgtgcgtacc gtgctggaag ccttaccggc ggcgtcacac
    29101 ccgatggatg ttatgcgcac cggcgtttcc gcgctcggct gcacgctgcc agaaaaagag
    29161 gggcataccg tctctggcgc gcgggatatt gccgacaaac tgctggcgtc acttagctcg
    29221 attcttctct actggtatca ctacagccac aacggcgaac gcattcagcc ggaaaccgat
    29281 gacgactcca tcggcggtca cttcctgcat ctgctgcacg gcgaaaagcc gtcgcaaagc
    29341 tgggaaaagg cgatgcatat ttcgctggtg ctgtacgccg aacacgagtt taacgcctcc
    29401 acctttacca gccgggtgat tgcgggcacc ggctctgata tgtattccgc gattattggc
    29461 gcgattggtg cactgcgcgg gccgaagcac ggcggggcga atgaagtgtc gctggagatc
    29521 cagcaacgct acgaaacgcc ggacgaagcc gaagccgata tccgcaagcg ggtggaaaac
    29581 aaagaagtgg tcattggttt tggtcatccg gtttatacca tcgccgaccc gcgtcatcag
    29641 gtgatcaaac gtgtggcgaa gcagctctcg caggaaggcg gctcgctgaa gatgtacaac
    29701 atcgccgatc gcctggaaac ggtgatgtgg gagagcaaaa agatgttccc caatctcgac
    29761 tggttctccg ctgtttccta caacatgatg ggtgttccca ccgagatgtt cacaccactg
    29821 tttgttatcg cccgcgtcac tggctgggcg gcgcacatta tcgaacaacg tcaggacaac
    29881 aaaattatcc gtccttccgc caattatgtt ggaccggaag accgccagtt tgtcgcgctg
    29941 gataagcgcc agtaaacctc tacgaataac aataaggaaa cgtacccaat gtcagctcaa
    30001 atcaacaaca tccgcccgga atttgatcgt gaaatcgttg atatcgtcga ttacgtgatg
    30061 aactacgaaa tcagctccaa agtagcctac gacaccgcac attactgcct gctcgacacg
    30121 ctcggctgcg gtcttgaagc tctcgaatac ccagcctgta aaaaactgct ggggccaatt
    30181 gtccccggca ccgtcgtacc taacggcgtg cgcgtccccg gaactcagtt ccagctcgac
    30241 cccgtccagg cggcatttaa catcggcgcg atgatccgtt ggctcgattt caacgatacc
    30301 tggctggcgg cggagtgggg ccatccttcc gacaacctcg gcggcattct ggcaacggca
    30361 gactggcttt cgcgcaacgc ggtcgctagc ggcaaagcgc cgttgaccat gaagcaggtg
    30421 ctgaccgcaa tgatcaaagc ccatgaaatt cagggctgca tcgcgctgga aaactccttt
    30481 aaccgcgtcg gcctcgacca cgttctgtta gtgaaagtgg cttccaccgc cgtggtcgcc
    30541 gaaatgctcg gcctgacccg cgaggaaatt ctcaacgccg tttcgctggc gtgggtggac
    30601 ggtcagtcgc tgcgcaccta tcgccatgcg ccgaacaccg gcacgcgtaa atcctgggcg
    30661 gcgggcgatg ccacttcccg cgcggtacgt ctggcactga tggcgaaaac gggcgaaatg
    30721 ggttatccgt cagccctgac tgcgccagta tggggtttct acgacgtctc ctttaaaggt
    30781 gagtcattcc gcttccagcg tccgtacggt tcctacgtca tggaaaatgt gctgttcaaa
    30841 atctccttcc cggcggagtt ccactcccag acggcagttg aagcggcgat gacgctctat
    30901 gaacagatgc aggcagcagg caaaacggcg gcagatatcg aaaaagtgac cattcgcacc
    30961 cacgaagcct gtattcgcat catcgacaaa aaagggccgc tcaataaccc ggcagaccgc
    31021 gaccactgca ttcagtacat ggtggcgatc ccgctgctgt tcggacgctt aacggcggca
    31081 gattacgagg acaacgttgc gcaagataaa cgcatcgacg ccctgcgcga gaagatcaat
    31141 tgctttgaag atccggcgtt taccgctgac taccacgacc cggaaaaacg cgccatcgcc
    31201 aatgccataa cccttgagtt caccgacggc acacgatttg aagaagtggt ggtggagtac
    31261 ccaattggtc atgctcgccg ccgtcaggat ggcattccga agctggtcga taaattcaaa
    31321 atcaatctcg cgcgccagtt cccgactcgc cagcagcagc gcattctgga ggtttctctc
    31381 gacagaactc gcctggaaca gatgccggtc aatgagtatc tcgacctgta cgtcatttaa
    31441 gtaaacggcg gtaaggcgta agttcaacag gagagcatta tgtcttttag cgaattttat
    31501 cagcgttcga ttaacgaacc ggagaagttc tgggccgagc aggcccggcg tattgactgg
    31561 cagacgccct ttacgcaaac gctcgaccac agcaacccgc cgtttgcccg ttggttttgt
    31621 gaaggccgaa ccaacttgtg tcacaacgct atcgatcgct ggctggagaa acagccagag
    31681 gcgctggcat tgattgccgt ctcttcggaa acagaggaag agcgtacctt taccttccgc
    31741 cagttacatg acgaagtgaa tgcggtggcg tcaatgctgc gctcactggg cgtgcagcgt
    31801 ggcgatcggg tgctggtgta tatgccgatg attgccgaag cgcatattac cctgctggcc
    31861 tgcgcgcgca ttggtgctat tcactcggtg gtgtttgggg gatttgcttc gcacagcgtg
    31921 gcaacgcgaa ttgatgacgc taaaccggtg ctgattgtct cggctgatgc cggggcgcgc
    31981 ggcggtaaaa tcattcccta taaaaaattg ctcgacgatg cgataagtca ggcacagcat
    32041 cagccgcgtc acgttttact ggtggatcgc gggctggcga aaatggcacg cgtcagcggg
    32101 cgggatgtcg atttcgcgtc gttgcgccat caacacatcg gcgcgcgggt accggtggcg
    32161 tggctggaat ccaacgaaac ctcctgcatt ctctacacct ccggcacgac cggcaaacct
    32221 aaaggcgtgc aacgtgacgt tggcggatat gcggtggcgc tggcgacctc gatggacacc
    32281 atttttggcg gcaaagcggg cggtgtgttc ttttgtgctt cggatatcgg ctgggtggtg
    32341 gggcattcgt atatcgtcta cgcgccgctg ctggcgggga tggcgactat cgtttacgaa
    32401 ggattaccga cctggccgga ctgcggcgtg tggtggaaaa tcgtcgagaa atatcaggtt
    32461 agccgcatgt tctcagcgcc gaccgccatt cgcgtgctga aaaaattccc taccgctgaa
    32521 attcgcaaac acgatctctc gtcgctggaa gtgctctatc tggcggggga gccgctggac
    32581 gagccaaccg ccagttgggt gagcaatacg ctggatgtgc cggtgatcga caattactgg
    32641 cagaccgaat ccggctggcc gattatggcg attgctcgcg gtctggacga caggccgacg
    32701 cgtctgggaa gccccggcgt gccgatgtat ggctataacg tgcagttgct caatgaagtc
    32761 accggcgaac cgtgcggcgt caacgagaag gggatgctgg tggtggaagg gccgctgccg
    32821 ccggggtgta ttcagaccat ctggggcgat gacgaccgct ttgtgaagac gtactggtcg
    32881 ctgttttccc gtccggtgta tgccaccttt gactggggca tccgcgatgc tgacggttat
    32941 cactttattc tcgggcgcac tgacgatgtg attaacgttg ccgggcatcg gctggggacg
    33001 cgcgagattg aagagagtat ctccagtcat ccgggcgttg ccgaagtggc ggtggttggg
    33061 gtgaaagatg cgctgaaagg gcaggtggcg gtggcgtttg tcattccgaa agagagcgac
    33121 agtctggaag accgcgaggt ggcgcactcg caagagaagg cgattatggc gctggtggac
    33181 agccagattg gcaactttgg ccgcccggcg cacgtctggt ttgtctcgca attgccgaaa
    33241 acgcgatccg gaaaaatgct gcgccgcacg atccaggcga tttgcgaagg acgcgatcct
    33301 ggggatctga cgaccattga tgatccggcg tcgttggatc agatccgcca ggcgatggaa
    33361 gagtaggtcg gataaggcgc tcgcgccgca tccgac
//
LOCUS       DAZKJR010000041        33394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKJR010000041 DAZKJR010000000
VERSION     DAZKJR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.041"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..185)
                     /locus_tag="ACHOWS_001972"
     CDS             complement(<1..185)
                     /locus_tag="ACHOWS_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFQ0047547.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRGETVV"
     gene            complement(790..1080)
                     /gene="nadS"
                     /locus_tag="ACHOWS_001973"
     CDS             complement(790..1080)
                     /gene="nadS"
                     /locus_tag="ACHOWS_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFQ0047548.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1081..1392)
                     /locus_tag="ACHOWS_001974"
     CDS             complement(1081..1392)
                     /locus_tag="ACHOWS_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0047549.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1621..2529
                     /locus_tag="ACHOWS_001975"
     CDS             1621..2529
                     /locus_tag="ACHOWS_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0047550.1"
                     /translation="MAQEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEAMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2593..3534)
                     /gene="fabY"
                     /locus_tag="ACHOWS_001976"
                     /gene_synonym="yiiD"
     CDS             complement(2593..3534)
                     /gene="fabY"
                     /locus_tag="ACHOWS_001976"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFQ0047551.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3579..4016)
                     /gene="dtd"
                     /locus_tag="ACHOWS_001977"
     CDS             complement(3579..4016)
                     /gene="dtd"
                     /locus_tag="ACHOWS_001977"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0047552.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDCA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4013..4885)
                     /gene="yihY"
                     /locus_tag="ACHOWS_001978"
     CDS             complement(4013..4885)
                     /gene="yihY"
                     /locus_tag="ACHOWS_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFQ0047553.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4879..5478)
                     /gene="yihX"
                     /locus_tag="ACHOWS_001979"
     CDS             complement(4879..5478)
                     /gene="yihX"
                     /locus_tag="ACHOWS_001979"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFQ0047554.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5619..6506)
                     /locus_tag="ACHOWS_001980"
     CDS             complement(5619..6506)
                     /locus_tag="ACHOWS_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFQ0047555.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6496..7845)
                     /locus_tag="ACHOWS_001981"
     CDS             complement(6496..7845)
                     /locus_tag="ACHOWS_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0047556.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7944..9377)
                     /locus_tag="ACHOWS_001982"
     CDS             complement(7944..9377)
                     /locus_tag="ACHOWS_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0047557.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9447..10457)
                     /locus_tag="ACHOWS_001983"
     CDS             complement(9447..10457)
                     /locus_tag="ACHOWS_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0047558.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10490..11377)
                     /locus_tag="ACHOWS_001984"
     CDS             complement(10490..11377)
                     /locus_tag="ACHOWS_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFQ0047559.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11402..12280)
                     /gene="yihU"
                     /locus_tag="ACHOWS_001985"
     CDS             complement(11402..12280)
                     /gene="yihU"
                     /locus_tag="ACHOWS_001985"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFQ0047560.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12453..13349
                     /locus_tag="ACHOWS_001986"
     CDS             12453..13349
                     /locus_tag="ACHOWS_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFQ0047561.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            13389..14177
                     /locus_tag="ACHOWS_001987"
     CDS             13389..14177
                     /locus_tag="ACHOWS_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0047562.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14237..15502)
                     /gene="yihN"
                     /locus_tag="ACHOWS_001988"
     CDS             complement(14237..15502)
                     /gene="yihN"
                     /locus_tag="ACHOWS_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0047563.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15604..16584)
                     /gene="yihM"
                     /locus_tag="ACHOWS_001989"
     CDS             complement(15604..16584)
                     /gene="yihM"
                     /locus_tag="ACHOWS_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFQ0047564.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(16592..17302)
                     /gene="yihL"
                     /locus_tag="ACHOWS_001990"
     CDS             complement(16592..17302)
                     /gene="yihL"
                     /locus_tag="ACHOWS_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0047565.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTRDIPETLVPLITVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17519..19342)
                     /gene="typA"
                     /locus_tag="ACHOWS_001991"
     CDS             complement(17519..19342)
                     /gene="typA"
                     /locus_tag="ACHOWS_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFQ0047566.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRSPKDD"
     gene            19714..21123
                     /gene="glnA"
                     /locus_tag="ACHOWS_001992"
     CDS             19714..21123
                     /gene="glnA"
                     /locus_tag="ACHOWS_001992"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFQ0047567.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLA
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            21297..22346
                     /gene="glnL"
                     /locus_tag="ACHOWS_001993"
     CDS             21297..22346
                     /gene="glnL"
                     /locus_tag="ACHOWS_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFQ0047568.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            22358..23767
                     /gene="glnG"
                     /locus_tag="ACHOWS_001994"
     CDS             22358..23767
                     /gene="glnG"
                     /locus_tag="ACHOWS_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFQ0047569.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23879..23989
                     /gene="yshB"
                     /locus_tag="ACHOWS_001995"
     CDS             23879..23989
                     /gene="yshB"
                     /locus_tag="ACHOWS_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFQ0047570.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(24179..25552)
                     /gene="hemN"
                     /locus_tag="ACHOWS_001996"
     CDS             complement(24179..25552)
                     /gene="hemN"
                     /locus_tag="ACHOWS_001996"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFQ0047571.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25741..26250)
                     /gene="yihI"
                     /locus_tag="ACHOWS_001997"
     CDS             complement(25741..26250)
                     /gene="yihI"
                     /locus_tag="ACHOWS_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFQ0047572.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(26369..26535)
                     /locus_tag="ACHOWS_001998"
                     /pseudo
     CDS             complement(26369..26535)
                     /locus_tag="ACHOWS_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26832..27464
                     /gene="yihA"
                     /locus_tag="ACHOWS_001999"
     CDS             26832..27464
                     /gene="yihA"
                     /locus_tag="ACHOWS_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0047573.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27788..30574)
                     /gene="polA"
                     /locus_tag="ACHOWS_002000"
     CDS             complement(27788..30574)
                     /gene="polA"
                     /locus_tag="ACHOWS_002000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0047574.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPVAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVVDEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            30938..31870
                     /gene="yihG"
                     /locus_tag="ACHOWS_002001"
     CDS             30938..31870
                     /gene="yihG"
                     /locus_tag="ACHOWS_002001"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFQ0047575.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCESLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(31911..33341)
                     /gene="yihF"
                     /locus_tag="ACHOWS_002002"
     CDS             complement(31911..33341)
                     /gene="yihF"
                     /locus_tag="ACHOWS_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFQ0047576.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVKLVDNELTQPLFIAAKNKSPMEATLRFAFGGSFSTTLDVAP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDLVLELSPLNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQINIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFVIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLIFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8421 a         8546 c         7967 g         8460 t
ORIGIN      
        1 actactgtct cacccctttg cctgccaccc tttgctttac gcaccggaaa ttgctgccgc
       61 tccagatatt gctcaaccgc ttcgcgtaaa attgcagatc gtgaaacctt acgccagccc
      121 ctaagcttgt tcagccgttt aattgcctct tctgataaat gaagaaaaac cgtattcatc
      181 gccattacca ataccttaca tatattactc attaatgtat gtgcgaaaat tacgccgaaa
      241 aatcatttaa aaaaagcgga tttttgcatg ctgttttgtt tttgcgagac gttttccaga
      301 aataataaga acgttgcagt tgtttcattt gttaatcaaa aatggttggg ttatcgccca
      361 ggatgagata tgggtgctat aaatatatta tatacaggga tgtttatttt ttgattggat
      421 ctttatttgc ataatctgaa tggtgtagcc gaaaaatgtc agcatcctaa cagcacccgg
      481 atttaaggtg aaattaatca tccatcacag accggtcagt actgctacca accggtctta
      541 acttgctcat cgttataatt ttgttggtaa cagttcggga aacaaggaat tccttccgga
      601 acacccccgg tatcatccga tagcattgac gtcaatcatc ggcgcgacga tgagtttttt
      661 taatcgcatc atagggtggc aacggtcagc acgattacgg gtcatagcaa aactcctgag
      721 caggtggcac tacgccgctt tcacaggagt tctgaatgct ggtgctttct gtatggcata
      781 accatgcctc taacggttaa gtgcttgcag cacatgttgc ggatcattgg caatcgctcg
      841 cagcaaagcc tttgcaggcc cggtcggtga acgtcgcccc tgctcccagt tacgcaacgt
      901 gtctacgctt acagaaataa gatcggcaaa tttagattgt gacaaccctg aagcgcggcg
      961 tatttctttg attttcatag catcaacttc aaaagtccgc gaaggctgtc gcgttccttc
     1021 aataatttca ttcatctggg ccatgctgtg agtcaaccgc tcaaaaagtg ccttatccat
     1081 ttaccacctc gtatttaaca accggagcat tgctttttcc tggggagaca gatcatcttt
     1141 aataccctta cgataaataa gcagtaatct gatctcataa tgtttaacct gatgaaagta
     1201 aataaccctg acaccagcac gtttcccctt tcctcctgac acccatcgaa cctttcttaa
     1261 cccaccagtt tcaggtatta cctcaccata gtcaggattc aaggccagga aaaactgaaa
     1321 acgactgaat tcatcgtcgg tcagcagttt ttgaacatct tcagtaaaaa tttccgtttc
     1381 gatgaagagc ataattatac gccattggct tagagtaaca atcctttttg tacgtcattg
     1441 acgtaccctt gaaaccacta gcttactgat agtgctccag caaaaccacg tctctgccgg
     1501 gtttgtcttt acatttgcga ggcgctttcc agatttttca cgcccgtgaa tttccctgta
     1561 tccatccagt aaaaacgggt gtataaaact gtacattcat acagcaaaat gagttctact
     1621 atggcacagg aaaaaggaat tgcagagtta gtcgaagagt ttatcgctgc gggtcgcccc
     1681 tcatcgcgtc aacaaagtat tgcgcagcgc agggagggct atatcgccag cgctgtcctt
     1741 gcgggtgaga cagaaacgcg ggtagatatt cagactatcg aacttgaagc gatgacctta
     1801 cgaatagtgt caccgctgaa tgccccgaca ttactcccga cgataattta ttactacggt
     1861 ggctgttttg ttagcggcgg gttcgcaact cacgacaacc agctgcgcca gttagcctat
     1921 tacgggcaat gtcgggttat tgccgtccag tatcggctgg caccggaaca tactttcccc
     1981 gccgcccatg atgatgcgca acgaggtgca gaattagtct ggcaacacgc cgaacgactc
     2041 ggcgttgata aacaacggat aactcttgcc ggtgacagtg cgggcggaca tctggcgctg
     2101 gtaactgctt tgcgattaaa gcgtgctggc gagtggcaac cggcacaact catcttaatt
     2161 tatcccatgc ttgatgcgac tgcgcatttc gaaagctaca tacgcaatgg tcacgattac
     2221 atcatcacac gcgacacttt actgagcggc tttgagatgt acctgccagg cattgaacgc
     2281 aggcatcctg aagccagccc tctttggcgc gatgatttca atggcttgcc acctgtacat
     2341 atcattaccg ctgaatacga cccactttgc gatgaaggtg aagcgttgta tcaccgcatg
     2401 acagggcaag gagtgcaatg cacctgccaa cgctaccttg gcgttatcca cggatttttt
     2461 cagttaggtg gtatcagtga ggctgcgcga tcggcgctac gggatgttgc ctggagagcg
     2521 ggaaggtaac tccggaccag ggcccagaaa aggttttcag attgaaaacc ttttcagtca
     2581 agtaaaacgc ccctactctt cttcgttccc gccctcttca tacgggccaa atggcttcgc
     2641 gggcagaacg atatacgtgc cttcaaacac tgcacccggc gtctcgtcgc caaagatttc
     2701 tacttgcatc tgcacccgtg cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa
     2761 ggcaccaagg tcggctaccg catgaggttt gccgctaatc ggcttgctgt agcggatatg
     2821 cgcatccgcc agaataatcg ttccgccgag gtggcgttcg cgcagcatca gccagataag
     2881 cccccaaccg gtgagcgttg ccagcgagaa taaactcccg gcaaacagcg tgtgatgcgg
     2941 attctgattg cctgtttccg gcatggtggt gataaatttt tgcccggtat attgctgaat
     3001 gcgcacgccc attttttcac tcagcgggat gtgttcatac cacgcctgtt gcagctgcgc
     3061 gcaccagtcg ccgcgatgca aaatatcatc cagagtggca acgggtttaa tcatcaaaaa
     3121 atggcgaatc ggcgtggtgg ttggcgtggt gatttctccc tgattaacaa accccagctt
     3181 ggcgaaaaac tccaccgcgt cttcgcgggc gctacaggtc acgcgcttaa caccttcctg
     3241 acgcgccaca gattcgaggg tcatcgccat cagcgttcct aaccctttgt cctgcacgtc
     3301 gggatgaacg gccataaagc gaatggacgc ttcattgtcg gcattaatat acagtcggcc
     3361 taccgccacc agattaccct gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca
     3421 cgcgtcgcgt tccgaacctt ttggttgatg caggggctta cgcaacattt cccagcgaaa
     3481 ctgatagtaa cgctctaatt cttcttctgt ttgtggaacc cgaaggtgat acatagcgat
     3541 actctctctt gtttcccgtg acaaccctgg aagctggctc atacctgcaa ccagaatgtc
     3601 acggggccat cattgaccag cgatacctgc atatccgcag cgaagcgtcc tgtttgcgtg
     3661 ttcatctctt gctgacggca gcgttcgacg aaatagtcat ataacgcctc tgcgcaatcc
     3721 ggtgatgcgc ctttggagaa acttgggcgc atcccccgtt cggtatctgc ggcaagggta
     3781 aactgggaaa ccaccagtac actgccgccc gcctgttgca cgttgagatt catcttgcct
     3841 tcggcatcgc taaagatgcg gtagccgagc acacgctcgc acagacggtt tgctttctgt
     3901 tcgtcgtcat ccttttcgac acccaataac accaaaagtc ccgcgccaat ttcacccgtc
     3961 acttctccct ccacggtgac gctggcacgg gttacgcgtt gaattaatgc aatcatggtt
     4021 cgtcgtcttc ttcttgttca gcagcttgtt ttaatttgcg gtattccccg agagtgacag
     4081 taatttccgc gccaagcaag acgatacacc atgtccagta gacccaaaca aagagaatgg
     4141 ggatcaccgc cagcacaccg taaatgagct gatatgatgg gaacatggtg atataaagcg
     4201 cgaaaccttt ctttcctgct tcgaacagga gcgcggcgac aaacgcgccg acaatcgcgt
     4261 cgcggttagg tacacggatg gtaggaacga tgctgtacag cagccagaaa gagatccacg
     4321 acaacagcag cggaaaaata cgcaacacat tatcgatgac agtattgaga tcgctcgccc
     4381 agcgcagaga gagcagatag gagctgatcg ccagactggc ccctgccaac agcggcccca
     4441 gcgttaaaat catccagtac acggcaaacg agtaaatttt tggtcgcgct cgtttactgc
     4501 gccagatggt attcaacgcg ctatcgatgg agtacatcaa caataacgcc gtgacgatca
     4561 gcccgcacgc cccgacggcg gtcattttgt tggaattggc gacaaactgt tcgatatatc
     4621 gctggataac atcgccagta gcaggcagaa agttggcaaa aataaagtga cgcaactgaa
     4681 tgctgacgtc ggaaaacatg ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg
     4741 gcactaatga gagcaacgac acataggcaa ggttacctgc cagggttgtc atgttgtcct
     4801 catcaatgcg ttgccagagt agttttagcc aggcccatag tggacgggta cgatgcctgg
     4861 ctttgtcctg aatggttttt agcataacac cttcgcgaaa tagtccggga tggtggtttt
     4921 atctttcacc agaatactag taatgcctag ctgattagct ccttctatat tatcggcgtt
     4981 atcgtcgaaa aagaccgtat cgtctggtga aaaaccttct gcctgcaaaa catgctgata
     5041 aattcgtgct tcaggtttgc gcatacccaa gtcttgcgac agatagatat ggtcagcagc
     5101 atcacggatt tccgggtatt cctccggcca gaaggtagta tgcaggcggt tagtattgga
     5161 aagcaccacc acgcgatgcc cctgctcacg cagtttatgc atgatggcga tcacttccgg
     5221 gcgcagcgca acaaataccg cctgccaacc gtgggagaac tgctcatagc ttagcggcag
     5281 ggccatctca tggcacagtg cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg
     5341 ctgatgaaac gcctcgccca tgtggaaact cttcttaagc gtagccagtg gaacacgcgt
     5401 taaatcgctc caggccccca gcacacggtt aaagtcgata tcgacaatca cattacctaa
     5461 atcaaagata tagagcattt ttgcctcctt tcgcgccaca agaaattaac tgtagcggga
     5521 aaggatgggt ttggctatgt ggcaataagg gtaatcttgt gatgctgatt gcgaaaaagc
     5581 agagtaattt cactggcttt ttcgcaatag atgaatggtt aagattcagg tgtaaattgc
     5641 caacgagttt cgatgaccag cggcttatct ggtgtcacaa attccggcgt tagatttatt
     5701 gcgttaggtg cctgagtcag cgggttaata caggtggcat cgggctgttt atcaaatacc
     5761 accagactgt ttgcagaaga ggtcatggtc atcgcgagtt taccaggcca cagtaactta
     5821 accttcaccc cgtcataaaa accaaaacag tcatcccagg gaccgacttg aggagagata
     5881 cgatttccag ttggcagttc atcactaccc gcttcttctt gccagtccgc atcaaaaagc
     5941 acctgcaacg attctgtatt ttgcggagtc agctttttcg caaaccacgg atgccagcct
     6001 gcggaggccg ggaatgtatc ggcatatgaa tggacttcca gttgcaaaac cagcgcatcg
     6061 ttctccagca aaaaagtctg aatcacttcc ccttgccagg gccacggtga agccaggggc
     6121 atacgtaaag tcagggaatc tgctgttttg tcgatgattt cccacgttga gtaacaagcc
     6181 ataccatgca acgcatgtgg tggtttattg gcgggtaaag aataacattg cccaccagca
     6241 ttcagcgtcg cattccccag tcgccctgcc cacggcacca ttgggaaaca gccatactga
     6301 aaagctctgc gctgcggctg ccattggcgt aatacttcag aaccaaatgc ctttaacgag
     6361 gttatcctgg caccatcatg cggataaatc tgcaggtgaa tattgccttt ttccagcgtc
     6421 actaactctc ctacaggccc aggccatggc gcgtcaaacg cctgttgtag cgccaggtaa
     6481 tgctcgtttt ttcgttcagt tttcatgaga ttgttccctg atgtagcggt aagaagcggt
     6541 ttcttgttca tcggttggct caatcagcca gacgcaaaca aaggaaagaa ctgagacagc
     6601 aaataaaaac agagcgacat accacggttt tcctccccct agcgccagca aacttgaggc
     6661 gataaggggg cttagtccgc caagaatcgc ggaaaattga taagcaaaag aaaggccggt
     6721 atatcgtact ttggtaccga acatgcgggt gaaaagtgtt ggctgcacgg caaacatcat
     6781 cgtcggacct aagttgtagc caaggatcat actgcaccag atgatgagcg tacttttgct
     6841 atccagtaat aagaagaaag gaaacgcgaa caggacacaa aatcctgctc ccgacaaata
     6901 cagacttttt tgacctatac gatctgataa ccatccaacc aatggacaac tgaaaatacc
     6961 gatcgccgag gcaatcaaca tacccgtcag gggaatatct cgcgacaatg ctaattggct
     7021 ggaaatatag acaataccaa aggcgttaat aatattagag gagacacttt ccgccagcct
     7081 cgcacccagt gccaataaaa tatttcgtgg atgttttttg aataattcaa tcaacggagg
     7141 aacggacttt tctttattat tcgtcgtttt ttgcttttga aaatccagcg tttcttcggt
     7201 atgcaggcgg ataaacagcc caacgatcaa catcaccgca gaaagccaaa acggaatgcg
     7261 ccatccccag gagagaaact gctccgaagt aagaaaatgg ttacagagag caaaaatacc
     7321 ggttgccagc ataataccca cagacgcagc cgtttgcggg agtgaaccta aaaatccgcg
     7381 tcgggattcg ggcgctgatt ctatggtcat caatgccgct ccgccgtact cgccaccaag
     7441 accaaagccc tgaatcaaac gcagcaccat taatacaagc ggtgcccaaa tgccaatttc
     7501 ctgatacgtc ggaataaaac caatcaaaaa tgtggagcaa ccgacaatag ccagcgtcca
     7561 gataagtgtt atttttcgcc cgattttatc gccaaaatga ccaaagacga tgccacctaa
     7621 aggacgagtc agaaaaccaa caccaaaggt cgcaaaggaa gctataagac ctataaaagg
     7681 atcgctgtct ggataaaaca gcggtgcaaa taccagtcct gctgccgtgc caaaaataaa
     7741 gaagtcatac cattccatta acgcgcctgc gaaactgccc ataacgacac gtctcatggc
     7801 tttaccggaa agcccttcac gcggtgcggg taacttttca gacataattt tcctctcctg
     7861 gtaggcgaga aatctgggat acgaaccact aaacgtgggc agtttgaatt aatgcgcatc
     7921 ggcttaaacc gatgcgcatt aaattatttc ttcaattcag ccagaacctc tgcgtcctca
     7981 gcatcaacat ttgcacccag tggttcccaa ttaatcaata cacaggtcag gaatcccagg
     8041 aatggcacga ttgccaacca gtaaaacgct tcagttttca acgcacccca cagcatcggc
     8101 cagaaaatga gtcctgcaat tgcccctgtg cgcataatag cgcgggcaaa tccgacccca
     8161 gtagggcgaa tagcgggagg ataagagagt gtggcaatgg tcatgcccag cccaccagga
     8221 cccgctgaat ggaagaaaat aattgatccc aataacccta aagacagcca gggattagtc
     8281 gttgccagtg cgcctaacga cagcaatgct aaggtcacaa cagcaaatcc atacatcgac
     8341 tgccggcgag tacccagtcg ttggagaata aacgagccga tcatcccgcc tgtcacccca
     8401 caaagggcat tcacgacggc agaaccggtt aacgcgccag taagcccgcc acttattatt
     8461 cccgccaacg taagaggaag ataaacacct acggcgttat attgccacgc ctgcatggtt
     8521 gccacgacac agccaagaat ggtgcgtttg cggtaacgat cattaaataa gatgccatat
     8581 ccaccactgt atttattctc tgctttttcc gagaatttcg cttctgttgt acccgatgcc
     8641 gcaacgcgag cctgaacacc ataacgtttt cccagaatgt cgcacgcttc ctggtaacgc
     8701 ccgacgcgtg cggcccacat tgcggattca ccaataaaga agtaacgcaa aatcatgaag
     8761 atgactgcaa aaatagctcc gatgaatatt ccgtaacgcc acaattgttc ttcagcgata
     8821 cccgacagaa gcagcggcaa aagcaccaaa taaacaacgg ttgtcgaaac ataccaggcc
     8881 atttgccaga ggttgaccga cgtgccggtt ttcttcgacg ttgaaccacg aagctctgcc
     8941 acggcattgg ttgccaaagg gaagtcgatg cctaaaccaa agcccatgat gacgcggcac
     9001 accagcaccc accagatact gggagcaata gctaccgctg cagcaccgat ggtgcacagc
     9061 cccatgccaa taataaatgc ccgcttttgc ccaaagcggt ttattactcg gttcgctaat
     9121 aaaccgccaa ttaacgcggc gattaatact gatgcattga cggttgcagc aagacccggc
     9181 gaaatgccga attgagcggc aatgtattta ttaccaaacc ccaccatcgc cgcctggtag
     9241 gcatcaatca atatgccgcc caaagcaata aaaataacgc cgatactggt gttgattgca
     9301 gagttcttat taacaatatc aattacgtcc tgtggccgac taattgaata agttgcagga
     9361 ctggtgattt gactcatcgc agtgtccttt ttaattgttg tagggtggcg ctgacgtgac
     9421 gcagcgccag acagagattt agaaaatcat tccggcgcaa attgcagcat gacctttatg
     9481 gagtcatttt gctgcgcgcg ttcgaatgcc gccgctgcat cctcaatggc aaataaatcg
     9541 gtaaccagtt cactggtatc aaaccgccca tccgccagcc attcgataac agcatcgaaa
     9601 tcttcaggga tgtacatgcc agagttgagc aagtcacgct cgaaacgttg catcatcggt
     9661 aatggtattt cacgcggccc acttggtacg cccatgcaca cgacggtgcc tccggcatat
     9721 acacgggtac atgcatcgtt aagcgtggcc tgcgctgcaa cgacatcaat cgcccccgta
     9781 aatcgcacat ctgcggccag ctcacctggt gcccaaactt ctgctgcgcc cagttttaac
     9841 gccagcgcgc gtttggcagc gtcaggttca atcacggtaa tcttacctgc ccccataatg
     9901 cgcagtgcct gcacgatgga taagcctatc gttccggcac caatcaccag tacgctttcc
     9961 agagaagctt tcggcattcg gttaacgcag tgacgagcac aagccgccgg ttcggcaaag
    10021 gccaacactt ttagcggtaa agaggctggt gcgacatggc aattacgcgc aggaacaatg
    10081 tgttgtgagc gagcaaagcc cggtgcgcgg aaaccagcaa cctgcggtgg ttcacaaaga
    10141 ttaaaacgtc ctgctttgca ggctcggcat tccatgcaag ccatgatggg atcgaccaca
    10201 acatgatcgc ccggctgtac atttttaacg tcgctgccca cttccgtaac gcgcgctgca
    10261 atttcatgac cggggacgac aggcggtttg gcaaacggat gaccgccttt cagaacatgc
    10321 agatccgaac cgcatatccc gtagaacacg ggcgcaatcc gtacttcgtg cggtttaagt
    10381 tgctggtgtg gtacttcaac atattgagtg gtgactttcc caatatctga aaataaaacc
    10441 tgggtaattt tatccattgt tattctccag aatcgatatc cataggggat tagcgttgcg
    10501 cgataccttc atcgacaatt tcagccagcc gttgcaagcg tggaacggag acatcgcgca
    10561 gcatggtttg tggatcttgt gcaccaacaa ccgacgccca aaccgctcgt ccagccagga
    10621 agcccgacgc accacctttc atggcaatac ttactgcacg gccaaaaata tcggcatcaa
    10681 cgccagaaga aagaattacc cacggcatct tcatctgatc gttaagttgc tgacaggcag
    10741 caagtaaggt tttttcatcg cctttgcccc ctaatggcat ctcggctttg taaagatcag
    10801 cctctgttcc accaagctct gcggcggcgg caacaatggc gctttcacga tcaaagtccc
    10861 aaccgcgacg aggtggacgc accacaggtt caataatgct aaccagcccg gcactacggc
    10921 agcgacgaac aaatttatca accattgcca gacgttcctc tgcgggttca tcttcacgcc
    10981 agagtacaag taatttcatt gccttagccc ccatttcacg ggctttatgc gggtcgacgg
    11041 acatatctat ttcaacgcta tcaaccggga tgccattacc tgggataaaa agatcggcag
    11101 cgacaatcag gccgcaagaa ttagccacag cgccctgctc gactatttgt tcaagacaaa
    11161 attgtttatc cgccagaacg gcagaagcat aaggagaaag gattcgggtc gcggcaactt
    11221 taaaatcggt gagtacactg tcggcgatgg gttttggctg ccctgctgcc gcaaacatca
    11281 ggcgcatggc ctcacgctga tcgacagcta acatggcgaa tccaccacct gggcgggaga
    11341 tatctttcag cgtaaatggg gtgaagtggg atgacataga aactcctgtt aatagataaa
    11401 attaaagacc agcctgctgg cgaacctgat ttagcagggc gctccagtcc tgccgccctt
    11461 tgccgtcttg acttgctgcg gcgtaataac tttctgctgc aatccccata ttgagcggcg
    11521 cccccgtttc ccgagcgacg tcaactgcaa tacgcagatc tttcaatgcc aggtccacca
    11581 tgaaggcggg agaaagatcg cctttcagca ctttgccagg ccaggtggtc gtaaaatgtc
    11641 ctttccctgc tgccgtacca ctcatcacct taattgcgac atcaagattt aatcccagtg
    11701 actcacataa cacagcggct tcggaggaca gcgcattaag cgcaatgctc atatagttgt
    11761 taattagctt cacgcggatc cccattcctg ggccaccagc ctcgaccagc tcattaccca
    11821 tgcacatgag aatcgggcgt gcacgcgcaa tctgctcatc agtgccgccc gctaaaatca
    11881 gcaatgtgcc atcaatcgca ttcactgacg ttctgcccac gggggcgtcc atcatattga
    11941 tgcctttttc ttgtaattcc cgtattaatg catcggtctg taagggatga atagtcgaca
    12001 tatcaatcac taacgcgtca gatgaaacag tttcacagac gcctttttcc cccaacagca
    12061 cctgtcgaac aatgtcccca ttgggaagca tagtgatgat aaattcagcg cttgctgccg
    12121 cttccgctgg ggtttgtgcc gcagtggccc cctgcgtaac tagcgcatcg acggcctgtg
    12181 tattgacatc aaatacctgt aatgaatgcc ctttttgcaa tagattgtta gccattgggc
    12241 tacccatctg ccccagcccg agaaatgcaa tggttgccat gaatactctc ctgtctgaca
    12301 ttgacaaaat aatgacaatt gaaaattcgt tttcgtctgt ttttgagcat atttgtaatt
    12361 tatgttcaaa aacacgacac gcatcactaa tttgatcatt tgtgtcattt aaattgacca
    12421 aagacgacaa aattaaacag gacaagacaa atatgaccag aattgcatgc gtgggtatca
    12481 cagttcagga caggatttat tctttgccca ctctgccgga gggcggcggg aaatatcagg
    12541 caaatcatta tcttgagatt ggtggcggcc cggcagcaac agccgctgtt gcaatcgcaa
    12601 agcttggcgt ggaggttgat ttcatcggtc gcgtcggcga tgacagttgt ggaaatacct
    12661 tacttgcaga acttgagggt tggggcgtca ataccgcatt ttgccgccga tatcctaacg
    12721 cccgttcgtc gcaatcagcg atcctcgttg atcagcatgg cgaacggatt atcgttaatt
    12781 atccaagtcc tgatctcggt actgatgccg aatggttaga agcgatcgat ttctctcgtt
    12841 atgatctgat tctggccgat gttcgctggc atagcggtac tgaaaaagca ttctcgctag
    12901 cgcgccttgc gggtgtgaca acgcttttag acgccgatat gacaccgcag gatatttctc
    12961 cacttgtcgc gcttgccgat cacgccgtgt tctcaacacc tgggctgaaa cgcatgactg
    13021 gcctgcagtc acctgaagaa ggtttgttcc aggcaacgac acaaacagca ggtaaagtgt
    13081 atgttacgct gggttctgaa ggatcgttgt ggattgaaga tggtcatttg tgtcaacagg
    13141 aggcattttc tgtcaatgtg gttgatacca ctggagcagg cgatgttttt cacggcgcac
    13201 tcgcggtagc gttggcagaa aaaatgccaa caaaagaggc tattcgtttt gccagtgcgg
    13261 ttgccgcgat gaaatgtacc cagcctggcg gtcgggccgg cattcctaat cgtgagcaaa
    13321 ccgaatcatt tttgtcactt tatgcgtaaa atggtaaaca tctaccgagg aggggcaagg
    13381 gagtattcat gagtatcatc gaagtgacag gtaatccacg ccatgaccaa ctcgttcatc
    13441 taatcgcaga acgcggttat atgaatattg aagaactggc acaactgctg gatgtttcaa
    13501 cgcaaaccgt gcgccgggat atccgaaaac tcagtgagca gggcttgatc acccgtcatc
    13561 atggaggggc cgggcgcgtc tccagcgtca tgaataccgc ttttgagcaa cgggaacttt
    13621 cgctcaccgc cgagaaacgg gcgatcgctg aggcagtcgc cgattacctt cccgaacgct
    13681 gtaccgtctt tatcaccatc ggtacaaccg tagaagccgt tgccagggca ttactcaacc
    13741 ggcgtgattt acgcattatc actaatagcc tgcgtgtggc acagattctt tataagaatc
    13801 aggatattga agtgatggtg ccgggaggca ctttacgcgc tcataacggt gggattatcg
    13861 gcccaggagc cgtggatttt attgaaggct tccgcgcaga ttatttaatc accagtatag
    13921 gtgccattga acatgacggc accctactgg aatttgatgt caatgaagcg ttagtcgcga
    13981 gaacgatgat taaacatgcg cggaatacat tgttagtggc cgatcatacg aagtttaccg
    14041 cgtctgctgc cgtttcaatt ggcaatgcac ggaatgtcag ggcttttttt actgatgccc
    14101 cgcctcccaa ttctttctgc cagttgttaa gtgaagagaa tgttgaactg gtggttgccg
    14161 agcaagaagt atcctgaaat tgaaacttcg gttgatacgg ttttcgttgg ccggattaag
    14221 cacatccggc cactaattaa gcttcagccg ccagcatctc tttcttacgt tggctaatac
    14281 gcttaaccag cattacggaa accaccgcac cacagaatgc gaagcaggcc ataatgccaa
    14341 acacgatttt gtagccaata atccccggat ttaaatccag aatgtagcca tacagactga
    14401 agcagaacat cgccggggcg taaccaataa agctacccaa cgccatcgcc gcgcctgttt
    14461 tattttcagc aatttttgct tcgccgatag gtgcaaaaaa tacggctcgc tgtgtgaaga
    14521 ctatagcgcc aaagcccagc gtacatgcca tccctaaata gaccggcata ctttcgtgcg
    14581 gcagcataat caacagtacg agcgcagcgg tacttatgat aaaggtgtag catagatatt
    14641 tactcggcga tttcaggatc ttatctgaaa tcatgccacc aatcggtcct ccaatcattt
    14701 tcagacagta ttggttaatg atgccgtaag cccccaccag agcaacgggc aatagataga
    14761 tgtttttcag gaatggaatg aagaatgtta ggccacagta aaccgcgtaa acgaagaaga
    14821 cgttgaaagc gataagccag atggttttat ctctcagcac cgaggtcata ctggtgtttt
    14881 gcgatgctcc gtcttctttt ttcacctcaa cggacggtgc ctcttctttg tcattcagga
    14941 caaagaaaat gataatgcct acggcaatca caataaggga atagaaccag atgcctgctt
    15001 taaaacccaa taagccactg ccaaaccagg taaatactgc caacgcagaa aatgccacca
    15061 cggtatcgac aatgccacgc cctgtttcga agaagccaaa caaccgacct tgttgttcgc
    15121 tattgcccaa tcgacttacc gacttgagca agaccggcca gttcatcatg tcgcaagtaa
    15181 cgccaaatag cgcccagaca aagaggatgc cccaataccc cggcattgtc gtcaaataaa
    15241 cacccagtaa tcctgtcgca atgagtgaga atgacatggt gtatctgcgc ggtagtttat
    15301 cggcaaaata aatagacaga aaaaagccca ctgtggtgac aaatgagttt accgacatag
    15361 cattaccaat ttgaccattg gtcaaatgga aatattcctg catcgggata taaaacgcat
    15421 ctttcagaga cggtaattta taaattgtac cgccacacaa tgttaataga ctaaataacg
    15481 cccatttctt tttcgtgagc ataacatccc ctaagcgatt tttaaaatca ctgttttaaa
    15541 ggcaataaga taccgaccat tcatatggaa tggttttatt aacagcgtat gagttaaaac
    15601 ttattagttg ttcagaagat ggtttgcctc ttgtttgatt tgttgtagta cattacgcac
    15661 atgatttatc tgattaattg catcttcttt ttctttacgt aaccgcgcat ccagtaaatg
    15721 attttctggc aatggtgttt cactcatctg gcgataaggg atccacggat tatcatcttc
    15781 gtcttcaaag cggaacgccg gcgcatagta atcaacctct tcttccaggc catatgccgt
    15841 cacctgcggc tcatcatcac ccaggcagat aaggtgcgtt aataacgctt tgaacggcat
    15901 atcaccctga cctgaaatac aggctttatg acccaggcca ccctcttctt taacgatcaa
    15961 ggcatctttg atatggacct gggtaatatg cggtgccatc gtttttaaag cgtcgatggg
    16021 atgctcattt gcattgatca tgttcgcaaa atcaaacagt aaggaaagag attccatctc
    16081 actttctttg accagcgaca ccagctcatg gctttttaaa tcttcatgct gttcgatcgt
    16141 aaaagtcagg ccgctgtcct gatacgtttc ccgtacatag gcaatgtcgt tagcgatgat
    16201 cgataatacg tcgcgcaaat taccttcata acgtggataa aaacgtacgg acgatgcccc
    16261 agttttcaac gcaatggcga cggcttcgtc aatagatgcc ttatctgaag cgctggtttc
    16321 aatatgaata tcaaggttca gacggcgggc tttatcccca aaggcagaga gttctttatc
    16381 gtccatatta ccaagagaaa aacgctcgcc atccagaaca tggattttga cgccacgtag
    16441 attattttca cttgcgatat caagtaaatc agcaggcaac actctttcca gccgcatatt
    16501 taaatgaaag gcataagcat gtaaataaag aggaagagtg tcgacacgtt gtagaatctt
    16561 tcttgcgtta tttattgtca ccataaaaca cctacagatt aatgatgagg tctacggcct
    16621 caaatcgcca atattcaata tctatctgga caatattgcc ttgctggtcg gcgcgatgct
    16681 tttcaattaa gatcgctggc atacccgcag aaccgccaag atacttgctg atatcgcccg
    16741 gcattctgac gggtttgaaa atcaattttt ttactaccgt ctctttttgg tagcactttt
    16801 cccagactgc agaaaatgag tggttttcaa gttgttcaat aaaacccggg gcaacttcag
    16861 gattaatata tgtttcgtga tagaaaactt tatgtccttc cagcgctccc cagccggtga
    16921 tgcgatagag ttcagttgat ggtgtcactg taatcagtgg cacgagcgtc tcgggaatat
    16981 ctctggtacg gcttttctcg gtaaaccccc aggaaggctc ccgcccttgc tcaattgcag
    17041 cccgctgaaa gctcgccgac agctctggac tgtaattaaa tcgcggctgg gtcacgaacc
    17101 accctttacg atccttacgg aagattttgg attctgcctc gaggttcaac aacgcctggc
    17161 gcagcgtcat acgtttaatg cccagcagtt ctcccagctc tctttctgaa gggagttttc
    17221 cacccggcgt ggtgatgcca tctttcaacc accgatccag tttctctttt gcattttcta
    17281 cggtggattg attctccgcc attgtttaat ccttttggtt taaaccagat gaggcatctt
    17341 acgcttacgc caaaaaagaa agcaattaac ggcgtatgac tttgcagata attgtgacaa
    17401 agctctctat tgacgtaatc acagacttat aagggagata ctcgcattgg tgtgcgctca
    17461 cgacaggtaa aaaaaaacct gccagcgatg gcaggttttt tatgactgaa aagcgaaatt
    17521 aatcgtcttt cggtgagcgg ttggcgcgac gacgatcgtt ttccgtcagg tgacgtttac
    17581 gaatacggat agaggtcgga gtcacttcta ccagttcgtc atcatcgatg aactccagag
    17641 cttgttccag agtcatgcgg ataggcggaa ccagaacaac ggcttcgtca gtaccggaag
    17701 cacgcatgtt ggtcagtttc ttaccggtca ggcagtttac agtcaggtcg ttagagcggc
    17761 tatgaatacc gataatctga ccttcgtaaa cttctgcacc gtgaccgagg aacagcttac
    17821 cgcgatcctg cagaccgaac agcgcgaacg cgaccgcttt accctgaccg ttagagatca
    17881 gtacaccgtt ctgacgctga cccacttcgc ccggacgtac gtcgtcgtag tggctgaagg
    17941 tggagtacag cagaccagta ccggaagtca tggtcatgaa ctcagaacgg aagccaatca
    18001 gaccacggct tgggatcacg tagtcgagac gtacgcggcc tttaccgtct ggattcatgt
    18061 ttttcaggtc gcctttacgt tcgcccagcg cctgcatcac agaaccctga tgctgttctt
    18121 caacgtccag cgtcacgttt tcatacggct cttgtttacg accgtcgatt tcacggaaga
    18181 ttactttcgg acgggatacc gccagttcga aaccttcacg acgcatgttt tcgataagaa
    18241 cagacaggtg cagttcgcca cgaccagaaa cgcggaacgc atcggcgtct tcggtttctt
    18301 ctacgcgcag cgcaacgttg tgtaccagtt ctttgttcag acgatccagg atctgacgag
    18361 acgttacgaa cttaccttct ttaccgcaga acggcgaggt gttaacgcag aagaacatag
    18421 aaacggtcgg ctcatcaacg gagagtgccg gcagcgcttc aacgttttgc gtgtcgcaaa
    18481 cggtgtcaga aatgttcagt tcgcccaggc ccgtgatcgc cacgatatcg ccagcttccg
    18541 ccagatcggt ttcgatacgt tccagaccga ggtggcccag cactttaccg actttcgcgt
    18601 tgcgggtttt gccttcgcta tcgatgatag taacctgctg gttcggcttc actttaccgc
    18661 gcttgatgcg gccaatgccg ataacgccaa catagctgtt gtaatcgagc tgagaaatct
    18721 gcatctggaa cggaccgtca aggtcaacgt ccggcgcagg aacgtggtca acaatcgcct
    18781 gatacagcgg ggtcatgtct tccgccatat cttcgtggtc cagacccgcg ataccgttca
    18841 gcgcagaagc gtaaacgatc gggaagtcca gctgctcgtc ggtcgcgtcg aggttaacga
    18901 acagatcgaa tacctgatcc acaacccaat caggacgcgc gccagggcgg tcaactttgt
    18961 tgataacaac aatcggcttc aggccgtaag caaacgcttt tttggttacg aagcgcgttt
    19021 gcggcatcgg gccgtcaaat gcgtcaacca ccagcagcac tgagtctacc atggacatta
    19081 cacgttcaac ttcaccaccg aagtcggcgt gccccggggt atcaacgatg ttgatacggt
    19141 aatcattcca tttgatagcg gtgtttttcg cgaggatggt aatcccacgc tctttctcca
    19201 aatcgttgga gtccatcacg cgctcttggg tttcggcacg agagtcgaac gtaccggatt
    19261 gttggagcag cttgtctacc agggtggttt taccatggtc tacgtgcgcg atgatggcga
    19321 tattacgcaa tttttcgatc acaactttgc ctcaggcatt agaaatagcg cgttattgta
    19381 cacggattaa tcgcactaca aaacaggatc acaaacatcc tccgcaaaca agtattgcag
    19441 agtccctttg tgatcgcttt cacggagcat aaaaagggtt atccaaaggt cattgcacca
    19501 atatggtgct taatgtttcc attgaagcac tatattggtg caacattcac atcatggtgc
    19561 agcccttttg cacgatgtta agcatgataa cgccttttag gggcaattta aaagttggca
    19621 cagttttcgc tttatctttt tacggcgaca cggccaaaat aattgcagat ttcgttacca
    19681 cgacgaccat gaccaatcca ggagagttaa agtatgtccg ctgaacacgt actgacgatg
    19741 ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca ccgataccaa aggtaaagaa
    19801 cagcacgtca ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga aggcaaaatg
    19861 tttgacggct cctcgattgg cggctggaaa ggcattaacg aatccgacat ggtgctgatg
    19921 ccagacgcat ccaccgcagt gattgacccg ttcttcgccg attctaccct gattatccgt
    19981 tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg ctctattgcg
    20041 aagcgcgccg aagattacct gcgttccact ggcattgccg acaccgtact gttcgggcca
    20101 gaacctgaat tcttcctgtt cgatgacatc cgtttcggat catctatctc cggttctcac
    20161 gttgctatcg acgatatcga aggcgcatgg aactcctcca cccaatacga aggtggtaac
    20221 aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg ttccgccggt agactctgct
    20281 caggatattc gttctgaaat gtgtctggtg atggaacaga tgggtctggt ggttgaagcc
    20341 catcaccacg aagtagcgac tgctggtcag aacgaagtgg ctacccgctt caataccatg
    20401 accaaaaaag ctgacgaaat tcagatctac aaatatgttg tgcacaacgt agcgcaccgc
    20461 ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg ctccggtatg
    20521 cactgccata tgtcgctggc taaaaatggc gttaacctgt tcgcgggcga caaatatgca
    20581 ggtctgtctg aacaggcgct gtactacatt ggcggcgtca tcaaacacgc gaaagcgatt
    20641 aacgccctgg caaacccgac caccaactct tacaagcgtc tggtcccggg ctacgaagca
    20701 ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat tccagtggtt
    20761 tcttctccga aagcacgtcg tatcgaagta cgtttcccgg acccggcggc taacccgtac
    20821 ctgtgctttg ctgccctgct gatggccggt cttgacggta tcaagaacaa gatccatccg
    20881 ggcgaagcga tggacaaaaa cctgtatgac ctgccgccgg aagaagcgaa agagatccca
    20941 caggttgctg gttctctgga agaagcactg aacgaactgg atctggaccg tgagttcctg
    21001 aaagccggtg gcgtgttcac tgatgaagcg atcgatgcgt acatcgctct gcgtcgcgaa
    21061 gaagatgacc gcgtgcgtat gactccgcac ccggtagagt ttgagctgta ctacagcgtc
    21121 taatagttga agttgtacta cccggcgcaa caacgccggg atttagttgc cgtggaaact
    21181 ttcagcccat ctctgcatgg gcttttttct ccgtcaattc tctgatgctt cgcgcttttt
    21241 atccgtaaaa agctataatg cactaaaatg gtgcaacctg ttcaggagac tgctttatgg
    21301 caacaggcac gcagcccgat gctgggcaga tcctcaactc gctgattaac agtattttgt
    21361 taatcgatga caacctggcg atccattacg ccaaccccgc cgcgcaacaa ctgctcgccc
    21421 aaagctcccg caaattgttt ggtacaccgt taccggaact gttgagctac ttctcattaa
    21481 atatcgagct gatgcaagaa agtctggagg cggggcaagg ttttactgat aacgaagtga
    21541 cgctggtcat cgacgggcgc tcgcatatcc tttctgtgac ggcccagcgt atgccggacg
    21601 gcatgatcct gctggagatg gcaccgatgg ataaccagcg ccgcttaagt caggaacagc
    21661 tacagcacgc ccagcaggtt gctgcccgtg atttagtgcg cggcctggca catgagatta
    21721 aaaatccgct tggcggttta cgtggcgcgg cgcagctgct cagcaaagca ttacctgacc
    21781 cgtcactact cgaatatacc aaagtgatta tcgaacaggc ggaccggctg cgaaatctgg
    21841 tcgaccgtct gttgggaccg caactgcccg gtacgcgcgt taccgaaagt attcataaag
    21901 tagctgaacg cgtggtgacg ctggtgtcga tggaactgcc ggacaacgtg cggttgattc
    21961 gggattacga ccccagcctg ccggaactgg cgcacgaccc ggatcaaatt gaacaggtct
    22021 tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc ggagggtggt gaaatcattc
    22081 tgcgtacccg cacagcgttt caactgacct tacacggcga gcgctatcgg ctggcggcgc
    22141 gaattgatgt ggaagataac gggccaggta ttccgcctca tttgcaggat acgctgtttt
    22201 acccgatggt cagcggccgc gaaggtggca ccgggcttgg cttatccatc gcccgtaatt
    22261 tgattgatca gcattcaggc aaaattgaat ttaccagttg gccaggtcat accgagttct
    22321 cggtttacct gcctatcagg aaataaaggt gacgtttatg caacgaggga tagtctgggt
    22381 agtcgatgac gatagttcca tccgttgggt gcttgaacgt gcgctcgctg gagcgggttt
    22441 aacctgtaca acatttgaga acggcgcgga ggtactggag gcgctggcga gcaaaacgcc
    22501 ggatgtgctg ctttcagata tccgtatgcc gggaatggac gggctggcgc tgctcaagca
    22561 gattaaacag cgccatccga tgcttccggt catcattatg accgcacatt ccgatctgga
    22621 tgctgccgtc agcgcctatc aacaaggggc atttgattat ctgcccaaac cgtttgatat
    22681 cgacgaagcc gtggcgctgg ttgagcgcgc catcagtcat taccaggaac agcagcagcc
    22741 gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc ggtgaagcgc cagccatgca
    22801 ggacgtgttc cggattatcg gtcggctttc gcgttcttct attagcgtgc tgattaacgg
    22861 cgagtccggc accggtaaag aactggtcgc tcatgccctg catcgccaca gtccgcgagc
    22921 caaagcgccg tttatcgcgc tcaatatggc ggctatcccg aaggatttga tcgaatccga
    22981 actgtttggt cacgagaaag gcgcatttac cggcgcgaat accattcgtc aggggcgttt
    23041 tgaacaggct gatggtggca cattattcct cgatgaaatt ggcgatatgc cgctggatgt
    23101 gcagacgcgt ttgctgcgcg tactggcaga tggtcagttt tatcgcgttg gcggctatgc
    23161 gccggtgaaa gtagatgtgc ggattatcgc tgccactcac cagaatcttg aacagcgggt
    23221 gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg aacgtcatcc gcgttcatct
    23281 gccgccactg cgtgagcgtc gggaagatat tccccgtctg gcacgccatt ttttacaggt
    23341 tgccgcgcgc gaactgggcg tagaagcgaa gttgctgcat ccggaaaccg aagccgcgct
    23401 gacgcgcctg gcgtggccgg ggaacgtgcg ccagctggaa aacacctgtc gctggctaac
    23461 ggtgatggcc gccgggcagg aagtgttgat tcaggatttg cccggtgaac tgtttgaatc
    23521 aacggttgcg gagagtactt ctcaaatgca accggacagt tgggcgacac ttttagcgca
    23581 gtgggcagac agagcgctgc gttccggtca tcaaaatctg ctttccgaag cgcagcctga
    23641 gctggagcgg acgttactga cgaccgcgtt gcgacatacg caagggcata aacaggaagc
    23701 ggcgcggcta ctcggctggg ggcgtaacac tctgacgcgg aagttaaaag agctggggat
    23761 ggagtgattc acggcttgtg tgtaaagatt gattattgag cgcaaattgc tggtgttttg
    23821 cgctttactg ttccgataag ttcagtatga tcttgcccgg aaaacgggga gagtcattat
    23881 gctggaatca ataattaatc tggtatcgag tggcgcagtt gacagccaca caccgcaaac
    23941 tgctgttgct gccgtgctgt gtgccgcgat gattgggctg tttagttgag tttcagattg
    24001 ctgatgaagt gctctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    24061 agcaatattt tcaacagatt ttgcaggcct gataagcgta gcgcatcagg caatcttgtt
    24121 ctcagcacat tgatggcgct ttcgttttct actttgtaaa cgaagcgcca ttcacaattt
    24181 aaatcacccg agagaactgc tgcatccgcg ctttctggcg cagataggta tcaaagcaca
    24241 tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac ctgtattccc ttctcatcca
    24301 catccaccag cccatctttt gctaacgggg cgagcagctt gagatcttcg gcaaagtaat
    24361 cagcgaagag caaatcccac tgttgctcaa tgggagcgta atccagacgg aagttgcaga
    24421 tgagcgactt aatcacatcg cggcgaatac agtcatcacg cgttagcgca ataccacgcc
    24481 acagcgcatt gccttgttca tccacttgct gatagtactg cttcaactct ttctggttct
    24541 gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc catccccagc agatcggtat
    24601 cgccctgagt ggtgtagccc tggaagttac gatgcagcac gccttcacgc tgggccaccg
    24661 ccagctcgtc atccggacgg gcaaagtgat ccataccgat aaactgatag cccgattgtg
    24721 tcaggaaggc gatggtttcc tgcaggatat cgagtttttg ctgcggactc ggcaggtcag
    24781 catctttgat tttgcgctga gcagcaaaaa tggtcggcag atgcgcgtag ttaaagacac
    24841 tcagacgatc ggggttcagc tccgccacac gtttcagggt aaaggcgaaa ctctccggcg
    24901 tctgtttcgg caggccgtaa atcaggtcga tgttggtgga ggtaaagcca atctcacgcg
    24961 catggttaag cagtgcaaag atgaactctt catcctgctc gcggttaacc agacgttgca
    25021 cttctttgtt gaagtcctgc acgcccatgc tcaggcgatt aaagccttcg gcgcgtaaat
    25081 gatcgagtac atccagttcg atttcccgcg gatcgacttc gatcgagatc tccgcatcgg
    25141 cattgaactg gaagttttcg cgcagcagct tcatcagacg gctgatttgc gctttattca
    25201 gatacgtcgg cgttccgccg ccccagtgca attggctgac gtgacgcccg gcgaacagcg
    25261 gtgcacgatg gacgatttct tgctccagcg cgtccagata ctgatcggcc ttgtgctgct
    25321 ggcgagtaac tatcttattg caaccgcaga agtaacaaag cttatggcag aacggaatat
    25381 gtacgtagag agataatgga cgctcaggat agcgcgccac ggcttgtaaa aacgcctgtt
    25441 cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt ggcccggaat
    25501 agttatattt ctggatcagg gccagatccc agtcgatttg ctgtacagac atgctcactc
    25561 cttccgatgg cgtctctggc ggctacggcg taccggcgga tgcccgtttc gagtcatcag
    25621 ccggttgcgc agccactttt gtcgccgcga caaccgctgt agtttaacga ataaccacac
    25681 cagataacat ataaccggaa gggttatcag cagaacggga aggcccacgg cggcaatccg
    25741 ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt cctcttcttc
    25801 gtcatcgtca taagagaggc cgagtttctg catcaactca tcaatacgat ccagtttggc
    25861 atccacccag gattgctctt cggcgctcag ggtttcgcct gcttccagac gttccagcag
    25921 cgcatccaga cgctcatccg tttccagtaa ctccaactcc gcctgcggtg aaagcatagg
    25981 tttctcactc ttcggtttgt gctgtttggt gactttttca gtcacgccca atggaatagg
    26041 ggttttactg ccaatacgtg gatctttcgg tgcgttctgg cctttgctgc ctgacgtggt
    26101 gttaccgccc gctgcgcggc tgcccggcgc atgaccacga cgttttttct gacgcttacg
    26161 gtcacgagct tcctgatcca gctcctcgcg tgtttttcga cgcgcttttg catgaccttt
    26221 gctgcgtgag tttgaagatg atggtttcat aatggttcgt cttttagccg ttttattcag
    26281 tatagatttg cggcggaatc taacagaaag caagcaaaga aaaaaggcga cagattactc
    26341 tgtcgccttt tttcctgact cataaccctt aacgggtctt acaatccttg caggccgcca
    26401 acacaccctg tttttccatt agccggaaca atccgtgttg attccatttc cgtttaatta
    26461 cgtctccgga cgtttatctt cctgacaatc ctctgtcttc gcctcctggc gctcctgacc
    26521 ctcatcctga gtcattgttc ctgttagcgt cctcgctcta tgggtgctac tttacgcctt
    26581 tgctttaaat aaacaatcaa ccgccatcag tatttgcaca ttttcagagt tttatataaa
    26641 agcaattcac tgatttatat agcttatttg acatacgtcg cagaattttc tctgctattt
    26701 cgcataaaga ctatcggcaa tgtctcacaa agcatatggc ttttacttac aacgctcacg
    26761 ccgaaaaatc cgccttttat tatggttcag gtataatcgc cgcaaagctt tgactgatgg
    26821 agacgaccgc tttgactaat ttgaattatc aacagacgca ttttgtgatg agtgcgcctg
    26881 atattcgcca cctaccttcc gataccggaa ttgaagtggc ttttgcaggc cgttccaacg
    26941 caggtaaatc cagcgcgctg aacacgctga ctaaccagaa aagcctggct cgtacctcaa
    27001 aaaccccggg gcgcacccag cttatcaacc tgtttgaagt ggctgacggc aagcgtctgg
    27061 ttgacttgcc tgggtacggt tatgcggaag tcccggaaga gatgaagcgc aaatggcagc
    27121 gcgcgctcgg cgagtacctc gaaaaacgtc agagcctgca aggtctggtg gtgctaatgg
    27181 atattcgcca tccgctgaaa gatttggatc agcagatgat tgagtgggcg gtagacagca
    27241 atatcgccgt gctggtactg ctgaccaaag cggacaaact ggcaagcggc gcacgtaaag
    27301 cgcaattgaa tatggtgcgt gaagcggtgc tggcgtttaa cggtgatgtg caggttgaaa
    27361 cgttttcttc gctgaagaaa caaggcgtgg acaagctgcg gcagaaactg gatacctggt
    27421 ttagcgagat gcagcctgta gaagaaacgc aggacggcga ataattttct tgccttaatg
    27481 cttgtgccgg atgtggcgta tcaggcatta cggatctttt ctttcgccca ataaaaaacg
    27541 ccccagtcat tactgactgg ggcggctaaa atattcagcc aaatccgatt acgtgaagta
    27601 aaaggtctga aagatagaac atcttacctc tgtaccctac gcgactaact ttactctttt
    27661 ttgttcagtt cagaaagcat tttttgtaat tttttttcag ttgttgcata ggaatttcta
    27721 atagccatca caaaacgtga cagcttatgt tgcttactta cgaaaaaagg gcatgttcag
    27781 gcgaatctta gtgcgcctga tcccagtttt cgccactccc cacttccacc agcagcggca
    27841 catccagacg ggtacagttt tccatcagtt gatgaatctg cttcgcgacg gcatcgacat
    27901 catctttatg aacttcaaat accagttcat cgtgtacctg catgatcata cgcacacgcg
    27961 gttgctcagc ctgtagccac gcatcaacgg caatcatcgc ccgtttgata atgtcggcgg
    28021 cggttccctg cattggcgcg ttaatggctg cacgttcagc cgctgcacga cgagccccat
    28081 tgctggattt gatatccggc agatacagac ggcgtccgtc cagcgtttca acgtagccct
    28141 gctctttcgc ctgagcacgg gtgcgttcca tatactccag cacgccaggg tagcgttcga
    28201 agtaaaggtc catgtacttc tgcgcttctt tacgtggaat gttcaattgc cgcgccagac
    28261 cgaaagcact catgccataa atcagaccaa agttgatcgc tttcgcgcta cggcgttgct
    28321 cgctggttac ggtttccagt ggcaaaccaa acacttctgc cgccgtagcc cggtggatat
    28381 cttttccttc cgcgaatgcg gtcagcaagc ctttgtcacg cgagagatgc gccataatgc
    28441 gcagttcaat ctgcgagtag tccgccgaga caatcacata atcctctggc gcaataaacg
    28501 cctggcggat acgacgacct tcttcgttac gcaccggaat gttttgcagg ttaggatcgg
    28561 ttgacgataa acgtcccgtt gcagttactg cctggtgata agaggtatgc acacgcccgg
    28621 ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt cgatttcagc ttcgccagac
    28681 cacgatactc cagaatcact tttggcaacg gatagtccag cgccagttct tccagtacct
    28741 cttccgacgt tgacggcgcg ccacccggcg ttttcttcag cggtttaatt ccctgttttt
    28801 caaacagaat ggtttgcaac tgcttggtgg aggaaaggtt aaattcttca cctgcaattt
    28861 catgcgcttt cttttccagc tcagccagac gaagagtgag ctcttcagaa tgattgtgca
    28921 gcactttcgg atcgatcttc acaccgttac gttcaatgcg tgaaagcacc ggcactagcg
    28981 gcatttcgat attctcgaag acgttcaacg gccctttgtg tttttgcaga tccggccaca
    29041 ttttcagatg caactgcaag gtgacatctg catcttcggc ggcgtaacgt ccggcctctt
    29101 cgagggcaat ctggttaaag gtcagttgat ttttgccttt acccgcaatc tcttcaaaag
    29161 tgatggtttt gtgcttcaac caacgttccg cgaggctgtc catatcgtga cgcccggcaa
    29221 cgctattgag aatgtaggac tccagcatgg tatcaaacgc aatcccacgc agctcaatgc
    29281 cgtaattcgc cagaataccg cgatcgtatt tcaggttttg cccaaccttc agcgcctttt
    29341 catcttccag cagcggtttt agcaactcga gtgcacgctc gcgagagatt tgatcgggcg
    29401 catcaagata atcatgagca accggaatat atgccgctac gcctggctcg atagcaaaag
    29461 aaagcccgac caggttagca gagatgttat caaggctgtc ggtttcggta tcaaatgcaa
    29521 ataccggcgc tttttccagc ttcgcaatcc acgctttcag tgtttcttca tcaaggatgg
    29581 tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc tggcgcttcg tctacaacac
    29641 tggtttcctg cggcttcgcg gctggttttg cccctttggc ctgtaaccat ttgcccgctt
    29701 cgacatcagc agtccagcgt ttgaactcat actttttgaa cagccctaac aactcttctg
    29761 ccaccggtgg ctgcacttcc agttgttcac aggtcagctc cagttcaacg tcggttttaa
    29821 tcgtcgccag ctggtaagag agataagcca cttctttgtt ttgctcgagc ttcgccgcca
    29881 ttgttttcgc gccacggaag ctcaacccag caattttttc tggctcggca tacagcgtat
    29941 ccagtccgcc aagaccttgt agcaatgctt gcgcggtttt ttcaccgacg cccggtacgc
    30001 cagggatgtt atcagaggag tcacccatca gcgccaggaa gtcgatgatc agttctggcg
    30061 gcacgccgta cttattcacc acctcttccg ggccgaggat ggtattcgtc atggtattga
    30121 taagcgtaat atttggcgtc accagctgcg ccatatcttt atcgcctgtg cttatcagca
    30181 ccggacgccc ggctttttcg gcttcccgcg ccagagtacc gataacgtcg tccgcttcta
    30241 cgccagaaac cgccagcagc ggcagtccca tcgctttaac catcgcgtgc aagggttcga
    30301 tttgtgcacg cagatcgtcc ggcattggcg ggcgatgtga tttgtaatgt tcaaacagtt
    30361 catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc tgcatgcgtc ggtttatatt
    30421 gcatgattag actgcgcagc atgttgagga caccatacat cgcaccggtc ggctcgcctg
    30481 cgctgttagt cagcggggga aacgcgtgat atgcgcgata aagataagat gaaccatcta
    30541 caaggataag tggattttgg gggatctgaa ccataatgtc cgtgcctgtt tatcagatta
    30601 tgggtaaagg atgccacaga caggatgaaa acatgagttt ttcaccgctt ttcgtgaaaa
    30661 tcttttgtag atcttctgga tcgctcgcaa atcgtcatgt ggataacttt gtgcataaat
    30721 tatttacatc gcttttaatt tattatcatg ctttttacag catgatattt gtctttgctt
    30781 ttcatacagt taaatataaa gccatcccta ataattgtga taatgggatt ttttctaatg
    30841 tggatatata tgaattattt ttctccttaa ggatcatccg ttatttgggg cgttttctgc
    30901 taaaatcggc gccttgcgct tctttagcgc actgtctatg gctaaccttt tgaataaatt
    30961 cattatgacg agaatactcg ctgcgataac ccttttgctg agtatcgtat tgaccatttt
    31021 ggtcactatt ttttgctccg tcccgatcat cattgccggg attgtaaaac tattgctacc
    31081 tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat tttatgatgt attgctggtg
    31141 tgaaagtctg gcggtattac tgcatctgaa cccacacctg caatgggaag ttcacgggct
    31201 ggaagggtta agtaagaaga actggtatct gcttatttgt aaccaccgta gttgggcaga
    31261 tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat atttcctcaa
    31321 gcagcagctt gcctgggtgc catttcttgg cctggcatgc tgggcgctgg atatgccgtt
    31381 tatgaagcgt tattcccgcg cttatttatt acgtcatccg gaacgacgtg gtaaagatgt
    31441 tgaaaccacc cggcgttctt gtgaaaagtt tcgcctgcac cccaccacca ttgtcaattt
    31501 cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa acgcactcca cttttcaaaa
    31561 cttgttgcca ccaaaggcgg caggcattgc gatggcgcta aatgtactgg gtaaacaatt
    31621 cgataaactg cttaatgtta cgctgtgtta tccggacaat aaccgtcagc cgttcttcga
    31681 tatgttaagc ggtaaactga cgcgaattgt cgtacatgtg gatttgcagc ctatttccga
    31741 tgagttacat ggcgactaca tcaatgacaa aagctttaag cgccattttc agcaatggct
    31801 gaactcactg tggcaggaaa aagaccggct tctgacgtcg ttgatgtcat cacagcggca
    31861 ggacaagtaa attttgctcg cctctgaatg agcagaggcg agtgagtatt ttaatgaaca
    31921 aaacgtccgg cacgagacat aaattcttct tcgctcatct cctgtccgtt aaaaacaact
    31981 ttacccggtg tataacgcag ttgcagcgag gcggtattgt tgtcaatcgt gattaactga
    32041 aacatctgac ctaatgtcat catcccgctg atttgtttat cagcccgctt ttgcgctttt
    32101 tcagcatcca ttccttcaga taaattaagc tgttttgcgg tttctgtgac aacattaagc
    32161 ggtaacttca catcaaaaat gagcgattta atatctttgt ttgtggatga tgaggatttg
    32221 gctgggtcgg caatactgat atccagattg gcatttagtt cgccgagtgc attcttccat
    32281 cttaccggtt gtttaatggt cggctcactt ttttgtaata acggtaaata ctctttgaac
    32341 aatgcagcat tcacttcttg cagagcaact tcatcgtttg ctaactcagg gtgtgcagca
    32401 agctgcttct gcatcgcaat gttatactgg atgacaaatt ggcgcaccgc tgagggatca
    32461 atagattcag caatcaatga ccactcacca ctaccgagtt gctgattccc ttttgttaac
    32521 ttatcaagtt cgtaggtcag attgacattg atataatctt tgtctttaac gcgatccagc
    32581 ctggtttttg caacgaaagc atcgatttgg gcaacgtctt ctccctgatt gataatgtta
    32641 atctgattga atttcgactc gctttcgcca accggaaatt tcttttcttc taatcgcgtt
    32701 agagaatcaa tggtaaaact tttcgccgtt actttattca atggcgataa ttccagaaca
    32761 agatcttcga ctttgccttc aatatccaga ttagacaatg aactaccatc accattaaaa
    32821 gtaaactggc cctcaccaaa agaaaacttt ccatattttg caggggcaac atctaatgtc
    32881 gtggagaatg agccaccaaa cgcgaatcgc aatgtcgctt ccataggaga tttatttttt
    32941 gcggcgataa atagcggttg tgtcagttca ttatccacta atttcacttt tgccgctgcc
    33001 agtgcgggga tgacattacc acgcattaac attgtgatgg gtaacggacc gtgctcaaca
    33061 tccacatcaa acgcaacttt ctggccaggt ttgatattga gatcaggtgc accgttatca
    33121 aatgtcattt gcatttgaaa acgagaatta aaaaagcctt tgtcaaaatt tttataactt
    33181 aacgtcatat cgtaggcatg ttcacctttc tttttagcat cgttaaaatc tttaataaac
    33241 ttttcgatac caggctgaat ttgcgtacct gtgtaccatg tgccaccacc ccagattacg
    33301 gctaacgcaa caataacacc tgtagctgac ttacgtatca tataaatatt ccctgataaa
    33361 acagcaaaac tttgaatatc cacttatgct gaaa
//
LOCUS       DAZKJR010000042        33351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKJR010000042 DAZKJR010000000
VERSION     DAZKJR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.042"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..2077)
                     /gene="prc"
                     /locus_tag="ACHOWS_002003"
     CDS             complement(29..2077)
                     /gene="prc"
                     /locus_tag="ACHOWS_002003"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFQ0047577.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQTFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(2097..2795)
                     /gene="proQ"
                     /locus_tag="ACHOWS_002004"
     CDS             complement(2097..2795)
                     /gene="proQ"
                     /locus_tag="ACHOWS_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFQ0047578.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(2892..3389)
                     /gene="msrC"
                     /locus_tag="ACHOWS_002005"
     CDS             complement(2892..3389)
                     /gene="msrC"
                     /locus_tag="ACHOWS_002005"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFQ0047579.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            3519..4802
                     /gene="yebS"
                     /locus_tag="ACHOWS_002006"
     CDS             3519..4802
                     /gene="yebS"
                     /locus_tag="ACHOWS_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFQ0047580.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPTTRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            4771..7404
                     /gene="yebT"
                     /locus_tag="ACHOWS_002007"
     CDS             4771..7404
                     /gene="yebT"
                     /locus_tag="ACHOWS_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFQ0047581.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            7484..8923
                     /gene="rsmF"
                     /locus_tag="ACHOWS_002008"
     CDS             7484..8923
                     /gene="rsmF"
                     /locus_tag="ACHOWS_002008"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFQ0047582.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            9041..9277
                     /gene="yebV"
                     /locus_tag="ACHOWS_002009"
     CDS             9041..9277
                     /gene="yebV"
                     /locus_tag="ACHOWS_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFQ0047583.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            9382..9573
                     /gene="yebW"
                     /locus_tag="ACHOWS_002010"
     CDS             9382..9573
                     /gene="yebW"
                     /locus_tag="ACHOWS_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFQ0047584.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(9574..10230)
                     /gene="pphA"
                     /locus_tag="ACHOWS_002011"
     CDS             complement(9574..10230)
                     /gene="pphA"
                     /locus_tag="ACHOWS_002011"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFQ0047585.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWFMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(10625..10966)
                     /gene="yebY"
                     /locus_tag="ACHOWS_002012"
     CDS             complement(10625..10966)
                     /gene="yebY"
                     /locus_tag="ACHOWS_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFQ0047586.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(10979..11851)
                     /gene="copD"
                     /locus_tag="ACHOWS_002013"
     CDS             complement(10979..11851)
                     /gene="copD"
                     /locus_tag="ACHOWS_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFQ0047587.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGIWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLMLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(11855..12229)
                     /gene="yobA"
                     /locus_tag="ACHOWS_002014"
     CDS             complement(11855..12229)
                     /gene="yobA"
                     /locus_tag="ACHOWS_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFQ0047588.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            12368..12598
                     /gene="holE"
                     /locus_tag="ACHOWS_002015"
     CDS             12368..12598
                     /gene="holE"
                     /locus_tag="ACHOWS_002015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFQ0047589.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            12700..13356
                     /gene="yobB"
                     /locus_tag="ACHOWS_002016"
     CDS             12700..13356
                     /gene="yobB"
                     /locus_tag="ACHOWS_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFQ0047590.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVSAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            13380..14042
                     /gene="exoX"
                     /locus_tag="ACHOWS_002017"
     CDS             13380..14042
                     /gene="exoX"
                     /locus_tag="ACHOWS_002017"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFQ0047591.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(14039..16099)
                     /gene="ptrB"
                     /locus_tag="ACHOWS_002018"
     CDS             complement(14039..16099)
                     /gene="ptrB"
                     /locus_tag="ACHOWS_002018"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFQ0047592.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(16308..16967)
                     /gene="yebE"
                     /locus_tag="ACHOWS_002019"
     CDS             complement(16308..16967)
                     /gene="yebE"
                     /locus_tag="ACHOWS_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFQ0047593.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(17294..17650)
                     /gene="yebF"
                     /locus_tag="ACHOWS_002020"
     CDS             complement(17294..17650)
                     /gene="yebF"
                     /locus_tag="ACHOWS_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFQ0047594.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQPDPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(17717..18007)
                     /gene="yebG"
                     /locus_tag="ACHOWS_002021"
     CDS             complement(17717..18007)
                     /gene="yebG"
                     /locus_tag="ACHOWS_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFQ0047595.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            18141..19319
                     /gene="purT"
                     /locus_tag="ACHOWS_002022"
     CDS             18141..19319
                     /gene="purT"
                     /locus_tag="ACHOWS_002022"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFQ0047596.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGIQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAGQVKVQG
                     "
     gene            complement(19375..20016)
                     /gene="kdgA"
                     /locus_tag="ACHOWS_002023"
                     /gene_synonym="eda"
     CDS             complement(19375..20016)
                     /gene="kdgA"
                     /locus_tag="ACHOWS_002023"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0047597.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(20053..21864)
                     /gene="edd"
                     /locus_tag="ACHOWS_002024"
     CDS             complement(20053..21864)
                     /gene="edd"
                     /locus_tag="ACHOWS_002024"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFQ0047598.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(22099..23574)
                     /gene="zwf"
                     /locus_tag="ACHOWS_002025"
     CDS             complement(22099..23574)
                     /gene="zwf"
                     /locus_tag="ACHOWS_002025"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0047599.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            23912..24781
                     /gene="hexR"
                     /locus_tag="ACHOWS_002026"
                     /gene_synonym="yebK"
     CDS             23912..24781
                     /gene="hexR"
                     /locus_tag="ACHOWS_002026"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFQ0047600.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAIVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            24909..26351
                     /gene="pyk"
                     /locus_tag="ACHOWS_002027"
     CDS             24909..26351
                     /gene="pyk"
                     /locus_tag="ACHOWS_002027"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0047601.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(26483..27454)
                     /gene="lpxM"
                     /locus_tag="ACHOWS_002028"
                     /gene_synonym="msbB"
     CDS             complement(26483..27454)
                     /gene="lpxM"
                     /locus_tag="ACHOWS_002028"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFQ0047602.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(27574..28896)
                     /gene="mepM"
                     /locus_tag="ACHOWS_002029"
     CDS             complement(27574..28896)
                     /gene="mepM"
                     /locus_tag="ACHOWS_002029"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFQ0047603.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(28912..29844)
                     /gene="znuA"
                     /locus_tag="ACHOWS_002030"
     CDS             complement(28912..29844)
                     /gene="znuA"
                     /locus_tag="ACHOWS_002030"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFQ0047604.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            29923..30678
                     /gene="znuC"
                     /locus_tag="ACHOWS_002031"
     CDS             29923..30678
                     /gene="znuC"
                     /locus_tag="ACHOWS_002031"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFQ0047605.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRHELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            30675..31460
                     /gene="znuB"
                     /locus_tag="ACHOWS_002032"
     CDS             30675..31460
                     /gene="znuB"
                     /locus_tag="ACHOWS_002032"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFQ0047606.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAIMFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(31610..32620)
                     /gene="ruvB"
                     /locus_tag="ACHOWS_002033"
     CDS             complement(31610..32620)
                     /gene="ruvB"
                     /locus_tag="ACHOWS_002033"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0047607.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(32629..33240)
                     /gene="ruvA"
                     /locus_tag="ACHOWS_002034"
     CDS             complement(32629..33240)
                     /gene="ruvA"
                     /locus_tag="ACHOWS_002034"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0047608.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         7970 a         8605 c         8319 g         8457 t
ORIGIN      
        1 aggcacaatt tcttgtgcct gattgatatt acttgacggg agcgggttgt tccgcgggtc
       61 tggctttttc aagcttcgcc agatcgagtg cgatattcac cgtctcatcc agataaggat
      121 ccggctcctg gtaatctttc ggtagatcat ccagtttctt caactccggt ttaccttcgc
      181 gtttaaagcg ttcgttcaaa cgcgccagac gcgtcgcatc atcttcatta ttctctttct
      241 cacgcacagc gtaattcaga gaaacgatat tgcgcttgtc cttcatggcg ttgaagcgcg
      301 cgatatcctt catgatgttc tggaactcag gatctttcgc gatacgcgca ttatgttcct
      361 tcagcagctc cggtccaaag gccgttaaat cgcctgattt cacataggtc gcggcatcga
      421 tgctatccca cggcagcgcg ttatcttcga atttctcacc cgtttccgtt tcttcattac
      481 ccgtcggcat gatgatgtct ggcgttacac ctttacgttg cgtactgccg ccgttaacgc
      541 gatagaattt ctggatcgtg tactgtacag atcccagcgc tggccattca ggacgtaaca
      601 tctgatcgta gatacggttc aatgaacggt attgctgaac ggtgccttta ccaaacgtcg
      661 gttcacccac aaccagcgca cgaccgtaat cctgcattgc cgcggcaaag atttctgaag
      721 ccgaagcact gaaacggtca accagtacca ccagcgggcc tttatagaag acctgtccgt
      781 cggtatcgct gtcttcacga actttaccgt tgttatcacg gacctgaaca atcgggccgg
      841 atggaataaa cagaccggag agcgataccg cttcggtcag cgccccacca ccattgctac
      901 gcaggtcgat gatgacgctg ctgacattct gtttttccag tttctgcagt tgcactttga
      961 catcgtctgt caaacccaca tagaagcccg gaatatccag cacgccgact ttctctttac
     1021 cgacggtctt caccgacatt ttaaccgcgc ggtcttcgag gcgaatacgt tcacgggtca
     1081 acgttacagt acgggtcttg gtccctttac cagcaggtaa aatttccaga cgaactttac
     1141 tgcccttcgg ccctttaatt aaggcaacca catcatcaag gcgccagcca atcacgtcaa
     1201 ccatcggctt gcctgtttga ccaacaccga caattttgtc accaacgctg atagctttac
     1261 tcttcgctgc cgggcctcct gccaccatcg aattgataac ggtgtagtca tcatccattt
     1321 gcagcactgc gccaatgcct tccagcgaca aactcatttc agtgttgaac tgttcggtat
     1381 tacgcgggga aagatagttg gtatgcgggt cgatttcacg cgcaaacgcc gtcattgcca
     1441 gcgagaaaac atcttcgctg ttggtttgcg ccagacgacg aatggcaaat ttgtagcggc
     1501 gagtcagggt ttcacgaatt tctttatccg tttttcctgc cagcttcagg cttaactcgt
     1561 cgaatttgac tttactgtcc cacagcgcgt tcaactcagc ctcgtttttc ggccagggcg
     1621 ctttgctgcg gtcaaggtta taagtgtcgt tgccggtgaa atccatcggc ttttccagta
     1681 ccgacaaagc gtactggtaa cgttcaaaac gacgcttttg cgccagattg tagagatcgt
     1741 agaaaacgtc gagtttgcct gaacgcagtt catcgcctaa ctcggttttc tttttcgcga
     1801 actgttcaac atcgcttgcc aacagcacgt tgtggctgta atcgagcaga ttcaggtagc
     1861 ggtcaaagat tttggccgaa aatgtctgat cgaggtcgaa ctggcgataa tgagaacggg
     1921 tgaagcgcga cgttacgcgc tcacttaccg tcgcatgctg cgtctcttcc tttaataccg
     1981 gaatttgatc agcacgcgtg atatcttcta cagcgaaggt ctggcctgct attgcaagca
     2041 ggccagctaa cgcggtaagc ctaaaaaaca tgttcatgcc tggcccggcc tccgtttcag
     2101 aacaccaggt gttctgcgcg cacaatcaaa gacatacccg aattcagctg gacgcggacg
     2161 ccgtctttgg tgatttctaa tacggtggca tccatcgcgt tttgacccgc tttcaccttc
     2221 agggcttgtc cgacagtcag agctgaaatg tcagaaaccg gggtgtgctg ttcttcgcga
     2281 ggtgctttta ctgtttttgg cgctttctct accggctttt gcgcacgagg tttacgttca
     2341 gcgccttctt tgcggcgtgg cgtggtcgga cgtggcttgc gttcgcggcg cggtgcgtct
     2401 tctttctcac cagcagctgc ggcagcttcg cgttttttcg cttgctgttc agcacgctgt
     2461 gcctgaacac gcgctttcgc ttcttcaagc tgcttgcgag catgctctac atgttgctcg
     2521 tccagctcac cgcatgggtt gccgtcaaga tcgacacgcg ttgcgccggg tttaacaccg
     2581 taaagataac gccagctcga agtgtagaga cgtaaagcgg atcgcaattg cgttttgctc
     2641 aggttcattt ccccggcaac acgatcgacc aaatcctgaa aaataccgat tttcagcgga
     2701 cgcgcttcac cttccgcact gaaacagtgg ggaaaacgtt cggccagaaa cgcgattact
     2761 tctttactgc tattcaactt aggttgattt tccatgaaat ttcctgatta caacggacgt
     2821 agccaacaag cgcaggcatg aacaggcgtc attataatga cgctatcagt aaatgctacg
     2881 ttatccgttg attatcctgc gacgctcgca aagaattttt tgtaatccgt cgttgcaagc
     2941 actttttcaa gctgtgccac aagctgacgt aagccttgct cgtcctcgtc tgtaaagcga
     3001 ccgaagacgg tactatcgat gtcgagaaca ccaataatct gatttttcac caccagcggc
     3061 agaacaattt cagaattact cgccgcatca caggcaatat gcccgtcaaa cacatgaaca
     3121 tcctcgatac gctgcacttg attgcgggca accgcagtgc cgcacacgcc gcgcccgacg
     3181 ggtatccgga cacaggcaat tttgccctga aatggtccga gtaccagtgt atcgtcctca
     3241 agcaaataaa aacctgccca gtttacgtca gtgagacgct catataacaa cgcactggtg
     3301 ttcgcaagcg ttgccagaaa actggtttct cctgccatca gcgcgttaaa gtcgcgattt
     3361 aaatccgcgt aaaattctat tttgttcatt atgtaatcac ttggttgtct taccaggaac
     3421 tgccagccta ttaaaataag cattaaatgc gttaatgctc aagatcattc caatcatggg
     3481 ttaagattaa tgttaattct ttttacattt ggcacgtcat ggctcttaac acaccacaaa
     3541 ttacgccgac aaaaaagata acagtgaggg ctatcggtga ggaactgccg cgtggtgatt
     3601 accaacgttg cccgcaatgt gacatgctgt ttagcctgcc cgagataaat tctcatcaaa
     3661 gtgcctattg tccgcgctgt caggcaaaaa ttcgtgacgg gcgcgactgg tcgctaacgc
     3721 gcctggcggc aatggccttc accatgctgt tgctgatgcc gtttgcctgg ggcgaaccgc
     3781 tgttgcatat ctggctgtta ggcattcgta ttgacgccaa cgtcatgcaa ggcatctggc
     3841 aaatgaccaa acagggcgat gcgataacgg gatcaatggt ctttttttgc gttattggtg
     3901 cccccctcat tctggtgacc tccatagctt atttatggtt tggtaaccga ctgggaatga
     3961 atctacgccc ggtactgctg atgcttgagc gacttaaaga gtgggtaatg ctggatatct
     4021 acctggtcgg cattggcgtt gcttctataa aggtacaaga ttatgcccat atccaggcgg
     4081 gtgtcggctt gttctctttt gtggcgttgg tgattttaac gacggtgacg ttgtcacatc
     4141 tcaatgtcga agaactgtgg gagcgatttt atccacagcg ccccactacg cgtagagacg
     4201 agaaactccg tgtctgtctt gggtgccatt ttaccggcta tcctgatcag cgtggtcgct
     4261 gcccgcgttg ccatatcccg ctacgcctgc gtcgccgtca tagtctgcaa aaatgctggg
     4321 cggcgctgtt agcgtcaatc gttttgttgt tacctgccaa cctgttgcct atttctatca
     4381 tttatctgaa tggtggccgg caggaagata cgattctttc cgggattatg tcgctggcaa
     4441 gtagcaacat tgccgttgcg ggaatcgtgt ttatcgccag tattctggta ccgtttacta
     4501 aagtgatcgt catgttcact ttactgttga gtattcattt taaatgccag caaggtttac
     4561 gcacacgcat tctgttactg cggatggtga cctggattgg tcgctggtcg atgctcgacc
     4621 tgtttgtcat atctttaacc atgtcgctga ttaatcgcga tcagatcctc gcttttacta
     4681 tgggaccggc tgcgttttat ttcggcgcag cggtaatttt gactattctt gctgtggaat
     4741 ggctggacag ccgcttactt tgggatgcac atgagtcagg aaacgcccgc ttcgacgact
     4801 gaagcgcaga ttaaaaataa acgccgtatc tcacctttct ggctgctgcc gttcatcgcg
     4861 ctaatgattg ccggttggct gatttgggat agttatcagg accggggtaa taccgtcacc
     4921 atcgacttta tgtcggcgga tggtattgtc ccgggccgca cccctgttcg ttatcagggc
     4981 gttgaagtcg gaactgtgca ggatatcagc ctgagcgacg atcttcgtaa gattgaagtc
     5041 aaggtcagca tcaagtctga tatgaaagat gcgctgcgcg aagagacaca gttctggctg
     5101 gtgacgccaa aggcctcact ggcaggtgtc tccgggctgg atgccctcgt cggtggtaac
     5161 tatatcggca tgatgccagg taaaggtaaa gagcaggatc actttgtcgc actcgatacc
     5221 caaccgaagt atcggctgga taatggcgat ctgatgatcc acctgcaagc ccccgatctc
     5281 ggttcactga acagcggttc attggtctat ttccgtaaga tcccggtggg taaagtctac
     5341 gactatgcca tcaatcccaa taagcaaggc gtggtgattg atgtcctgat cgagcggcgt
     5401 tttaccgacc tggtgaaaaa aggtagccgt ttctggaacg tttccggcgt tgatgccaac
     5461 gtcagtatca gtggcgcgaa ggtgaaactg gaaagtctgg cggcactggt taacggtgcg
     5521 attgccttcg attcacctga agagtcgaaa cctgccgagg cggaagatac ctttggtctg
     5581 tatgaagatc tcgcccacag ccagcgtggc gtaataataa aactggaact gccgagtggg
     5641 gccggattaa ccgccgactc gacgccgtta atgtatcagg ggctggaagt cggacagctg
     5701 actaaactgg atttaaatcc tggtggtaaa gtcaccggag aaatgaccgt tgatcccagc
     5761 gtcgttacgc tgttacggga aaatacccgc atcgaattac gtaatccgaa attatccctt
     5821 agcgatgcca atctcagcgc cctgctgacc ggaaaaacct ttgagttggt gcccggcgat
     5881 ggtgagccac gcaaagagtt cgttgttgtg ccaggcgaaa aagccctgct acaagagccg
     5941 gatgttctga cactgaccct gacagcacca gaaagttacg gtattgatgc tggtcagccg
     6001 ctcattctcc acggtgtgca agtaggccag gttattgatc gtaaactcac cagcaaaggc
     6061 gttaccttta ccgtcgccat cgagcctcag catcgcgaac tggtaaaagg cgatagcaaa
     6121 tttgtcgtca acagccgtgt cgacgtgaag gtggggctgg atggcgttga gtttctcggt
     6181 gccagcgcct cagaatggat taacggcggg atacgtattc tgccgggcga taaaggcgag
     6241 atgaaagcca gctatccact gtatgccaat ctggaaaaag cgctggagaa cagccttagc
     6301 gatttaccca ccacaaccgt aagtttgagt gcagagacgc tgccggatgt gcaggcagga
     6361 tcggtagtgc tgtaccgtaa atttgaagtt ggtgaagtta ttactgtgcg tccgcgagct
     6421 aacgcgtttg atatcgatct gcatattaag ccggagtatc gcaaccttct gaccagcaac
     6481 agcgtgttct gggcagaagg tggggcgaaa gttcagctaa atggtagtgg tctgaccgta
     6541 caggcatccc cgctctccag agcattaaag ggggctatta gcttcgataa cctcagcggt
     6601 gccagcgcca gtcagcgtaa aggcgacaag cgtattctgt atgcttccga aacagcggcc
     6661 cgtgcggttg gcgggcaaat tacgcttcac gctttcgatg ccggaaaact ggcggtcggg
     6721 atgccaattc gctatctcgg tattgatatc gggcaaatcc agacgctgga tctgattacc
     6781 gcgcgcaatg aagtacaggc aaaggcggta ctttacccgg aatatgtcca gacctttgca
     6841 cgcggtggta cacgcttctc agtggtcaca ccgcaaattt cggcagctgg cgttgagcat
     6901 cttgatacta tcctccagcc gtatatcaac gtcgaaccag gccggggcaa tcctcggcgc
     6961 gactttgaat tacaagaggc taccataact gattcgcgtt acctggatgg cttaagcatt
     7021 attgttgaag cgccggaagc cggttcgtta ggcatcggta cgcctgtgct gttccgtggt
     7081 ctggaagtcg gtacggttac cggaatgacg ctggggacat tgtcagatcg cgtgatgatt
     7141 gcgatgcgca tcagtaaacg ctatcaacac ctggtgcgta acaattccgt cttctggttg
     7201 gcatcgggtt acagtctgga ctttggtctg acgggcggcg tagtgaaaac cggcaccttt
     7261 aaccagttta tccgtggcgg catcgccttc gccacgcctc cgggtacgcc actggcaccg
     7321 aaagcccagg aaggcaagca cttcctgttg caggaaagtg aaccgaaaga gtggcgtgaa
     7381 tggggaactg cgcttcccaa ataatgccca ctgctccggc gtgcctgcgc cggagcgttt
     7441 atgctaaact gcgcgcctgt ttttttgcca gtggtatatg ctcgtggccc aacacaccgt
     7501 ttatttcccg gacgcctttc tgacacaaat gcgcgaagcg atgccttcga cgctctcttt
     7561 tgatgatttt cttgccgcct gtcagcgccc gttgcgccgc agcattcgcg ttaatacgct
     7621 gaaaacctcc gttgctgatt tcctgcaatt aactgctcct tatggctgga cgcttacgcc
     7681 aattccgtgg tgtgaagaag gtttctggat tgaacgcgac agtgaagatg cattgccgtt
     7741 gggtagtacc gccgagcatt taagcggcct gttttatatt caggaagcca gttcaatgtt
     7801 gcctgttgcc gccttgtttg ctgacggtaa tgcaccacag cgggtgatgg atgtcgctgc
     7861 cgcgcccggc tccaaaacga cgcaaattgc cgcgcggatg aataacgaag gggcaatcct
     7921 tgccaatgag ttttccgcca gtcgggtaaa agtgttacat gccaatatca gccgctgtgg
     7981 catcagtaat gttgcgctca cgcattttga tggccgcgta tttggtgcgg cagtgccaga
     8041 aatgttcgat gccattttgc tggacgctcc ctgctccggc gaaggcgtgg tgcgtaaaga
     8101 tcccgatgcg ctaaaaaact ggtcaccaga aagcaatcag gaaatcgcag cgacccaacg
     8161 ggaactgatc gacagcgcct ttcatgcatt acgtcctggc ggtacgctgg tttactcgac
     8221 ctgtacctta aaccgggaag aaaacgaagc cgtttgcctg tggctgaaag agacttatcc
     8281 cgacgcagta gagtttttac cgcttggcga tctcttccct ggtgcaaaca aggcgctgac
     8341 cgaggaaggc tttttgcatg ttttcccaca aatttacgac tgcgaaggct tcttcgttgc
     8401 tcgtctgcgt aaaactcagg cgattcccgc cttacccacc ccaaaataca aagtgggcaa
     8461 tttcccgttc agcccggtga aagatcgcga agccggacaa attcgtcagg cggctgcaag
     8521 tgttggctta aactgggatg aaaacctgcg actctggcag cgtgacaaag aactgtggtt
     8581 gttcccggtg ggcattgaag ccctgatcgg taaagtccga ttttctcgct tggggattaa
     8641 acttgccgaa acgcacaaca aaggttatcg ctggcagcat gaagccgtta ttgcccttgc
     8701 ctcccccgac aatgtgaacg cttttgaact gacaccgcag gaagcggaag agtggtatcg
     8761 cgggcgcgat gtttacccgc aagccgcgcc agtagcggat gatgtattgg ttactttcca
     8821 gcatcagccg attggtttag ccaaacggat tggttcgcga ctgaaaaaca gctacccgcg
     8881 tgaactggtg cgcgatggga aactttttac cggtaacgcc tgacagcgca caaaaaaagc
     8941 gcactttttg actggcacat tcggctgcct caactaggct gaaaaatggt gcgatcggac
     9001 tggtcgtacc acaaccggca gctaaatgga gagcacgaag atgaaaacca gtgtgcgcat
     9061 aggcgctttt gaaatcgacg acggcgaatt acacggcgaa tcgccgggtg atcgaacgtt
     9121 aaccattcct tgtaaatcag accccgattt atgtatgcaa ctggatgcct gggatgctga
     9181 aaccagtatt cctgccctgc taaatggcga acactctgtc ctttaccgta cccgttacga
     9241 tcaacaatct gatgcctgga ttatgcgtct tgcctgatcc aaaaagaacc cgtcggcatg
     9301 gcgggttatt tgtcctggtt atttccccgt tgtaaaatct ctcctaaact taacggtacg
     9361 gcaccacact tcggggatga aatgttcgcg ctggtacttt ttgtttgcta cctggatggc
     9421 ggttgtgaag atattgttgt ggatgtctac aacacggaac agcagtgtct ttattctatg
     9481 agcgatcaac ggatccgcca tggcggttgt tttccaatcg aggattttat tgatggtttc
     9541 tggcgaccag cacaggagta cggtgatttt taattattgc aattgcacaa gagtcagttc
     9601 gcccccaaag acagcaccgg tatcaatata atgcaggttg ccaatatcca cgcgatgtcg
     9661 caacggtgta tgaccaaacc agaaatgatc agcaccttta attccctgcc cttttgggcg
     9721 ttcacctaat cgcgagcggc tccacaagac ctgatgcaaa tcaacgtcct tttgccattc
     9781 ataaacatca tccggataat cggcatgggc aataacatgt ttgccggtgc gactgtgtac
     9841 ttcaagaata aagggcaaat gctgacattt ttccagcgcc gttttcgctt gtttctgttg
     9901 attatctgcc agcgcaataa accagtcgcc gccattcatg aaccacaaag acatctgctg
     9961 ggatgccagc gcatccatcg ccatctgttc atgattgcct cttaccgcac gaacccaatg
    10021 ttgttccagt aactgcaggc aacgtaaact ttgcggccca cgatcgataa cgtctcccac
    10081 tgagataagt aaatctcgcc acggatcaaa acgacaatgc cataatttgc ggcgcaactg
    10141 ctcaagacaa ccgtgtatat cgcctgaaag ccagatatgt cgccattgat gacccgcaat
    10201 tctctgataa acgggcgcag gctgtttcat caatattttc ctcccgcgct aaagatcaca
    10261 taatcttaac aagaatgtta aaaaacgctg gacgcagaca gtagagtgtg tgttatggtt
    10321 gactataaag tcagcgaagg gaatgcttct ggcttttaac agataaaaag agaccgaaca
    10381 cgattcctgt attcggtcca gggaaatggc tcttgggaga gagccgtgcg ctaaaagttg
    10441 gcattaatgc aggcttagtt gccttgctct ttaagaatag atgacgacgc caggttttcc
    10501 agtttgcgtg caaaatggtc aataaaaagc gtggtggtca tcagcttaaa tgttaaaaac
    10561 cgcccgttct ggtgaaagaa ctgaggcggt tttttattgg aaatcaaaag gctattttag
    10621 gtaattaaca gagtttttca gctcgctcta taaacggtgc cagactcatt ttttcgccgg
    10681 gattgttagg atcatcaatc tgaatcaccg aaatgggttg ggctttagtc ttcccactgg
    10741 caacttcctt ttgtgcgata tcgtttaaag gatactgcac gagggtactt gggttgatga
    10801 catacaaagc attacccggt cggcaagtca gcatcacctc ttcgcgatta aacgcccatt
    10861 tgtctttacc cacttcaaaa cggctgacgg taatcacctg cggtgccgcc agcgccgctg
    10921 cagaactggt gagtaacaga aacgccagaa tacttttttt catcattttc gtaatccatc
    10981 aaaaaggttc gagagtcgca agcaggctga tgatcaccag cactaccgca cctatcgccc
    11041 attcgagttt agtcatccac acaaaccagg gagccgcacg atcttcatcc tgcctcatgc
    11101 gcggtacaag aacataacga ttagccagcg cgatgaccac cataatcatc accaggatgg
    11161 ctttgagcat taagagttgg ccccagtaag tcgtaagcgt ggggggaaat ccggtaatca
    11221 acaatgcatt aagcacgccg cttgccagta cgccgatcac cgcaaaatgc ccgcaccagg
    11281 aaaaacgcat cagcgcctga atagcctggt gtcgccaacg gcctttgatg agttgcatac
    11341 accagagcac cggcagtaat cccccaaacc acgccgccgc acagatcagg tgaattgcgt
    11401 gattagtctg atggattttc gccgttaccc cttcattcag cgtcgcatgc ccgactcccg
    11461 ccagcagaat aaattgcgcg gtagtgagca taaatagcaa acgtggcata ttctgcggtt
    11521 gcataagggc aacgatcaac gttacgaggg cgagaacaat ttgccataac cagatgccac
    11581 caaactgcgt ttgcaaaaca gccagccaaa tgtttggcga aaatacatct gaccatcccg
    11641 ttcccatcaa cccgccctga acggcgagca ttgcagtggc actgatcaaa ctccagacgg
    11701 cagcgtgctg ttgtaaacgc agaaaacgct tcgtcagcaa gcgacgaatc gttaaggggg
    11761 ccagccaggc accgtacatt gcgaagccaa aaaccagcat caacgaggtg aaatggataa
    11821 atcgcagcgc gatccaggta aacgccagca tgatttattt cacgctaaag gtgtagtgtc
    11881 ctttcgtttt atgcccgtcc accgaaacaa catgccagtc aacggtgtag gttccgggtt
    11941 tcagcgaatc ggccagtgga acaatcagtt gcttttggtc ctgctcattc cgcttcgccg
    12001 gtaatgtttt aatattttcg ttttttggcc ccgtgatttt tgcaccactg aatcctgttt
    12061 caacgccttc cgagaagttt aaagtgattg cctgcggggc agctgtcact tgcgcatttg
    12121 ccgcaggata ctgatgcgtt aaatgcgcat gtgcccagac ggaaggtgtc accaacgaag
    12181 tggtcaggat tgccagcgcg tagcgaaggg agcgtgcagt tgaagccata ttatctattc
    12241 ctttttgtaa taactttttt acagagcata accttgtcta atgtctgagt cgaggatcat
    12301 caattccggc ttgccatcca ggctcactct tagtaacttt tgcccgcgaa tgatgaggag
    12361 attaagaatg ctgaagaatc tggctaaact ggatcaaaca gaaatggata aagtgaatgt
    12421 cgatttggcg gcggccgggg tggcatttaa agaacgctac aatatgccgg tgatcgctga
    12481 agcggttgaa cgtgaacagc ctgaacattt gcgcagctgg tttcgcgagc ggcttatcgc
    12541 ccaccgtttg gcttcggtca atctgtcacg tttaccttac gagcccaaac ttaaataaaa
    12601 cttatacaga gttacacttt cttacataac gcctgctaaa ttatgagtat tttctaaacc
    12661 gcacccataa tttgcagtca ttttgaaaag gaagtcatta tgtcgttctg gaaagttgca
    12721 gcagcgcaat atgagcctcg taaaacctca ctgaccgaac aggtagctca tcatctggag
    12781 tttgtgagtg ccgccgccag gcaacaatgt cagctattag tttttccatc gctttcttta
    12841 ctggggtgtg attattcgcg acgtgccctt cctgccccac ccgatctctc gctgttagac
    12901 ccgctttgtt atgctgcaac gacctggcga atgaccatca ttgccggcct tcccgttgaa
    12961 tataacgatc gctttattcg tggaattgcg gtgttcgcac cgtggcgaaa aacaccaggg
    13021 atctaccatc agagtcatgg tgcatgtctg ggccgtcgtt ccagaaccat cacggtggtc
    13081 gatgaacaac cggaaggtat ggatatggac ccgacctgtt cactgtttac gacagggcaa
    13141 tgtctggggg aacccgacct actggcatct acccgccgct tacaattttt ttcacatcag
    13201 tactcaattg ccgtgctgat ggcaaatgcc cgtggtaaca gtgcactatg ggatgaacat
    13261 ggtcgtctca tcgttcgcgc cgatcgtggt tcattattgt tagtcggtca gcgttcatca
    13321 cagggttggc aaggcgatat cattccatta cgctaggctt tttcggcctg gagcatgcca
    13381 tgttgcgcat tatcgataca gaaacctgcg gtttgcaggg agggatcgtt gagattgcct
    13441 ctgttgatgt cattgacgga aaaatcgtca accccatgag ccacctggtg cgccccgatc
    13501 gtcctattag tccacaagcg atggcgattc atcgcatcac cgaagccatg gtcgccgata
    13561 aaccgtggat tgaagatgtg atcccacact attacggtag tgaatggtat gtcgcgcaca
    13621 acgccagctt tgaccgccgc gtactgcctg agatgcccgg tgagtggatt tgcactatga
    13681 aactggcccg tcgtttgtgg ccagggatca agtacagcaa tatggcgtta tataaaacac
    13741 ggaagctcaa tgtacagacg ccgccgggcc tgcatcatca ccgcgcgttg tatgactgtt
    13801 atatcaccgc tgcgttgctt atcgatatta tgaacacctc cggctggacg gcagaacaga
    13861 tggccgatat caccggacgt ccgtcgttga tgacgacctt cacctttggc aaataccgtg
    13921 gcaaagcggt ttccgacgtt gccgaacgcg atccgggcta tctgcgctgg ttgtttaata
    13981 acctggacag catgagcccg gagctgcgtt taacactgaa acattatctg gaaaatactt
    14041 aatcctcagg cgtagcgggt aatgttccct gcgccagcgc gaccagaaaa gcatattcca
    14101 tcgctacgcc ttcgtacgat ttaaagcgac cagatttacc gccatgacct gagtccatgt
    14161 cggtacagag caataaaaga tggtcatcgg ttttcagctc gcgcaattta gcgacccatt
    14221 ttgccggttc ccaatattgc acctgagaat cgtgcaaacc ggtcgttacc agtaaatgcg
    14281 gataagcctg tgcggtgacg ttgtcatatg ggctgtagct tttcatgtac tcgtaatatt
    14341 gcggatcctg cggattcccc cactcttcaa actcaccagt ggtaagagga attgattcat
    14401 caagcattgt tgttacaaca tcaacaaacg gtacctgggc gataacgccg tggaataatt
    14461 ccgggcgttg attaattgca acgcccatca acatgccccc cgcacttccg cccatcgcat
    14521 aacagagcga aggagagcca tagcccagtt ttaacaatgc atcgcaggca tcaagataat
    14581 cattaaacgt atttttcttc ttcagaaatt taccgtcttc gtaccattgt tgccccagct
    14641 caccaccgcc gcgaacatgg acaatggcgt agacaaagcc acgatctaac aaactcaagc
    14701 ggctaaaact gaaatcggca tcaatacttg cgccgtaaga accgtagcca tacaccagca
    14761 gcgggttgtg tcctttgcga aaatgtttgc gatgatagac cagcgaaacc ggaacttcga
    14821 cgccatcacg ggcgactatc cacaggtgtt cactgcggta attcgccgca tcaaaaccag
    14881 gaacttccgt ttgttttaat acacgacgct caccggtatc catatccagt tcaaacaaag
    14941 tatctggtgt ggtcatggaa gaataaccat aacgcaatcg cgcggtttca ggttctggat
    15001 tgtaggcaat ccaggtcaca taggccggat catcaaaggc aatgccaatg acttcccggg
    15061 tcttgcggtt gatttggcgc aaactggtta acccgcgctg acgctcttca accaccagcc
    15121 agtcggtaaa cagcgtaaac ccttccagca taatgttatc acgtggcgga attaactctt
    15181 cccactgttg ctcatcacgc atacgggtac ggtataagcc aaagttttta ccgtggcggt
    15241 tggaacgcag ataaaaccga tgctggtagt gatcaaggct gtattcgtga tctttgcggc
    15301 gaggcagaaa aacaaacggc tcggcatcgg ccatttccgc gtccagcagg cgaacttcac
    15361 tggtggtggc gctggccaga tgaatgacca cataatgctt cgaggtcgtt ttatgcaggc
    15421 tgacgtaata ggtatcgtct ttttcttcgt aaatcagttt atcttgcgat gctggcgtac
    15481 cgatggcgtg acgccagacc tgataaggca gcagcgtcac tggatgcttg cgaacatagt
    15541 agaaagtcca ggagtcattt gcccagacaa agctgggttc aacgttatcc agcagttccg
    15601 ggtaccagtt acctgtttcc agattacgaa aacgaatgcc atactgccgt cgggaaagaa
    15661 aatcttctgc cagtgccata atggtgttat ctggcgtaat cgccattccg cccatcgaat
    15721 aaaactcact atgagccgcg cgcttgttgg catcgagcaa tgtttcccac tcgtcccact
    15781 cttcactgaa cgccgattga cgctggtaga tagcatattc acagcctggt tcataaatat
    15841 gccgatagcg gtagccattt ttgatgtagg gcgcagaaac ttctcgttgc ggaatgcggt
    15901 cgatgatttc ctttaagatg cgatcctgca aggcttgttg cgaggccatc acccgatgac
    15961 cgtaactatt ttcttgttgc agatagtcca ggactttcgg ctgagaacgc gtatcgtccc
    16021 gcagccagta gtaattatcg atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc
    16081 gggcggcttt tggtagcatg ttattgttct ttctggttga aacatcttat aagggtggca
    16141 aaactcaccg gggatgcaag cgaaacaggg tagtcattgc ttagatgatg acaggtaatg
    16201 gcgcggatat cgaatgttat gcagacagag aaaatcagcc tgctcaaatg gctgtgcggt
    16261 tctggataac ccgaaataat cagcttcagg ctatccagag agcggaatta ctccgccaaa
    16321 gtacgttttt gctgttcgag atcgcgttca atgccttcac gaacatcctg ggggattttc
    16381 agcgcatcac ccagtgcatt caggtaactg cgttccataa aatggtcaat atcaatagcc
    16441 gcgcagctca ggaaatagat ttccagcgcc tcttcttcat tgcggacccc ggtagccagg
    16501 cgttgtggat ccagcggttg ttcgattgcc tgctcaatga gtacacgccc cttctcttcc
    16561 acaccggctt ctcgcaattg ctggtcgatt gccgcacgtt ctttggcatc aatatggcca
    16621 tcacttttag cggcaaagac cagcgcaagg atcaaacgtt ccgtacgctc atccagcggc
    16681 gtactttgcg cgccaaactg cggttcatcc tgatgcgccg cgcgaatttt atctttgtat
    16741 ttattccaca gcaccgtacc cgctaccgct ccgccgccaa ccagtaacgc gtttgtgcca
    16801 tatttcgtaa gaagtttacg tgctgattta tttgcgacca gcagcccagc cagtccgcct
    16861 aatgcgccag gcactaaaag tttgcccaat ccctgatccg cagacgagga ggtagaagaa
    16921 cggctttgcc caagcaggga ttgcagttga tttaaccagt tagccatatt tgctcctcaa
    16981 taaccatttt tagtgcctgt agcatagcgg atgaggatgt cagaaaatgt atattgagac
    17041 aaaagatgcg caaataagcg gggcggtagg ccgttattca aagaaattcg cgccaggtaa
    17101 ggtgttcact gtatataagg tgataagggt attttaggtg aaagtagttt gacgttttga
    17161 gtggaactgt ttgatgctga ttgcccggtt cgacgctgct gcgtcttctc aggactacga
    17221 ttgtgttgcg ttgtagggcg gacaaggcac tcgcgccgta tccgccaacg gtcaatcgaa
    17281 ttatcagaag ccattaacgc cgctgatatt ccgccattcc cgctttgcag tccacgctga
    17341 cctggtaatg aatatcggca cttttaccac gtacggttag cggtactgac catttatcat
    17401 ctttcccctg aatgtcctgc aaactgaccc atgccacggg atcgggctga ccgacaattt
    17461 tttgatcatc agcccagcgc gccacgcgat tttgttggta atcacgtttt acgctcgcgg
    17521 caattccggc agcatccaga ccttcacact ttgggaaagt gaccgacttg ctggtttcat
    17581 tattggcagc gaaaactgat gcgcaggcag aaaccaacaa cagccctaaa aacgcccctc
    17641 tttttttcat gtttttctcc atagcacaat gattcaggag aaagcatggt acaaattgtc
    17701 aggagcgcaa gttgcttcag gccgcgtgtg aggcatcttc ctcttcggat tcagcaccga
    17761 cgacctgtgg ggacggtaat ttgccggttt tcagaatggt gctcagcaca tctttttgtt
    17821 ctgccagcca tagcgaaagg gcttcacgtt gctcgtcttc catttgcact ggagaatttg
    17881 tcagccaggt gtcaagcaga tccgccgtat caagcatttt gtcataagca tcggcttcct
    17941 ttttgctggt aaacgacatt ttctcttcgc cctcacgaat gactacgtat ttaacttcaa
    18001 ccgccatttg cagcctctca taataactgt gattttatac agtatatttc ttctcgattg
    18061 ggaaatcaac atcaacaata aagacacgcg caaacgtttt cgtttatact gcgcgcggaa
    18121 taaatcaggg gatattcgtt atgacgttat taggcactgc gctgcgtccg gcagcaactc
    18181 gcgtgatgtt attaggctcc ggtgaactgg gtaaagaagt ggcaatcgag tgtcagcgtc
    18241 tcggcgtaga ggtgattgcc gtcgatcgct atgccgacgc accagccatg catgtcgcgc
    18301 atcgctccca tgtcattaat atgcttgatg gtgatgcatt acgccgtgtg gttgaactgg
    18361 aaaaaccaca ttatatcgtg ccggagatcg aagctattgc caccgatatg ctgatccaac
    18421 ttgaagagga aggactgaat gttgtcccct gcgctcgcgc aacgaaatta acgatgaatc
    18481 gcgagggtat ccgtcgcctg gcagcagaag agctgcagct gcccacttcc acttatcgtt
    18541 ttgccgataa cgaaaacctt ttccgcgagg cggttgctga aatcggctat ccctgcattg
    18601 ttaaaccggt gatgagttcc tccggcaagg ggcagacgtt tattcgttct gcagagcaac
    18661 ttgctcatgc atgggagtac gctcagcaag gcggtcgcgc cggagcgggc cgcgtaattg
    18721 ttgaaggcgt cgttaagttt gacttcgaaa ttaccctgct aaccgtcagc gcggtggatg
    18781 gcgtccattt ctgtgcaccg gtaggtcatc gccaggaaga tggcgactat cgtgaatcct
    18841 ggcaaccaca gcaaatgagc cagctggctt tggaacgtgc gcaagagatt gcccgtaaag
    18901 tggtgctggc actgggcggt tatgggctgt ttggtgtcga gctgtttgtc tgtggtgatg
    18961 aggtgatttt cagtgaggtc tcccctcgcc cacatgatac cgggatggtg acgttaattt
    19021 ctcaggatct ctcggagttt gccctgcatg tgcgagcctt tctcggactt ccggttggcg
    19081 ggatccgtca gtatggtcct gcagcttctg ccgttatcct gccacaactg accagtcaga
    19141 atgtcacgtt tgataatgtg cagaatgccg taggcgcagg catacagatt cgtttatttg
    19201 gtaagccgga aattgatggc agccgtcgtc tgggtgttgc gctggcgacc gctgagagtg
    19261 ttgatgaagc gattgagcgc gcaaagcacg ccgccggaca ggtaaaagta cagggttaaa
    19321 cccggcaaaa aaacgctaca aaaatgcccg atcctcgatc gggcattttg acttttacag
    19381 cttagcgcct tctacagctt cacgcgccag cttagtaatg cggtcgtaat cgcccgcttc
    19441 cagcgcatct gccggaacca gccaggaacc accgatgcac agcacgcttt tcagcgccag
    19501 gtagtcacgg tagttagccg gagaaatacc acctgtcggg cagaaacgga cctgggagaa
    19561 cggacccgcg atcgcctgca gggctttcac accgccgtta gcttcagccg ggaagaattt
    19621 gaactctttc aaaccgtagt ccatacccag catcagttcg gaaacagtgc tgatccccgg
    19681 aatcagagga atagtccctt cggtagcagc tttcagcagc ggctcggtca gacccgggct
    19741 aattgcaaac tgtgcacccg cttcagtgac ttctgccagc tgctgtggat tcagcaccgt
    19801 accggcaccc acaatcgctt caggcacttc tttggcgata gcacggatag cgtcaactgc
    19861 gcactcggta cgcagagtca cttccagaac gcgcacccca ccagcaacca acgcttttgc
    19921 catcggcacc gcgtgttcca gtttttttac cacgataacc ggtacaaccg ggccggtggt
    19981 caggattgat tctgcacttg ttttccagtt tttcatcaga gttttctctc gcctgattac
    20041 aaatttgtcg tcttaaaaag tgatacaggt tgcgccctgt tcggcaccgg acagtttttc
    20101 acgcaaggcg ctgaataatt cacgtcctgt tcccacgcgt gacgcgctca ggtcaggaat
    20161 gtgtggttcg cgagcagcca gttccgcttc gtctaccagc agcgtcagtt cgcctgtctg
    20221 tccattcaca cgaatgatgt ccccgtcgcg cacttttgcc agcagcccgc catcgtaggc
    20281 ttctggtgtt acgtggatag ctgacggcac tttacctgaa gcgccggaga gtcgtccatc
    20341 agtaactaac gcaattttga aacaccggtc caataataca ccaagtggcg gcatgagttt
    20401 atgtaattct ggcattccgt tcgcttttgg cccctgatga cggacaacaa cgacacaatc
    20461 gcggtccagc aaacccgctt caaaggccgg cataacgtca tgctggcttt caaaaacaac
    20521 cgcaggcgct tcaatcacct ggttctctac cggcacggca gaggttttca taaccgcacg
    20581 gcccaggtta ccgcttaaca cttttgtccc accatgatga gagaaaggtt gttcgaagga
    20641 agcgatcaca ttgctgtcga gtgatttttc cgccccttcc cgccagtcca gttcaccatt
    20701 attcagccat ggttcaaggg tataacgaga cagaccaaag cctgccaccg tattgacatc
    20761 ttcatgcagc aggcctgctt tgagcagttc acgcaccaga accggtacgc cacctgccgc
    20821 ctggaagtgg ttaatatcgg ccggaccgtt cgggtagaga cgtgccatca gcggtacaac
    20881 atcagaaagg tcagagaagt catcccagtt aatctgaata ccggctgcgc gcgccatcgc
    20941 caccaggtgc atggtgtggt tagtggaacc accggtcgcc agcagtgcaa cgataccgtt
    21001 caccaccact ttctcatcga tcatcttacc gatcggcatc cattcattac cattaccggt
    21061 catgcgcgta acctggcgcg cagctgcagc ggtcaaagca tcgcgcagcg gagaatccgg
    21121 atgaacaaaa gaagagcctg gcaactgcat ccccataaac tccaccacca tctggttggt
    21181 gttggcagta ccgtagaaag tacatgttcc cggcgcatga taagacgcgg cttctgactc
    21241 cagtagtgcc atgcggtcca ctttaccttc ggcataaagc tggcgaatac gcactttttc
    21301 tttatttggc aaaccgcttg ccatcggtcc agacggcaca aacaccgcag gcaaatgccc
    21361 aaacgacagg gctgccatcg tcagacccgg gacaatcttg tcgcacacac cgaggaacag
    21421 agcaccatca aacatgttat gggacagccc caccgccgca gacatcgcaa tcacttcgcg
    21481 gcttaacagc gacaattcca ttccatcctg cccctgggtg acaccatcac acatcgccgg
    21541 aacaccgcct gcaacctgac caaccgcatt tgcttcatgc agagctttac gaatgatttc
    21601 tggatagtgt tcataaggct ggtgcgcgga gagcatgtcg ttataggagg tgatgatggc
    21661 gatattgtta cgcaacatgc ttttcaaaga ggctttgtct tctggctggc aggcagcgaa
    21721 accgtgtgcc aggttaccgc atgccaactg cgaacgatga acggtcgaag ttttcgcttg
    21781 ttctatccgg gcgaggtaag cagagcgagt ctcgcgcgaa cgttcaatga ttcgatttgt
    21841 tacgcgtaac aattgtggat tcataaaggc tcctgaaatt gagttgtcag agcaggatga
    21901 ttcacaacac gtttcattca gaggattcat gactgaaacg cctgtaaccg gagctcatag
    21961 ggcaaaaacg tttcagtcag tgtaataaaa aaagcctcgt gggtgaatcc gcacgaggcc
    22021 tgaaagtgta aaaattgttc tacaatctgt gcaagatcat gttaccggta aaataaccat
    22081 aaaggatgag cgcagatatt actcaaactc attccaggaa cgaccatcac gggtaatcat
    22141 cgccactgag gcaacgggtc cccaggttcc ggcctgatac ggtttcggcg catcattgtc
    22201 catcgcccac gcctcagtaa tggagtctac ccatttccag gcttcttcca cttcatcgcg
    22261 acgtacgaac agtgcctgaa taccacgcat ggtttccagc agcagacgtt cataggcatc
    22321 tgccagatgc gtttgattaa aggtttctga atagctcaga tccagcttgg tgatttgcag
    22381 gttatgttta tggtcaaggc caggaacttt atttagtacc tggatatcca cgccttcatc
    22441 aggttgcaga cggatagtca gtttattctg cggcagatcc tgccacgact ctttaaacag
    22501 attcagttca ggtgttttga aatagaccac gacttcagaa catttggtcg gcagacgttt
    22561 accagtacgc aggtagaatg gcacaccggc ccagcgccag ttatcaatgt cgacgcggat
    22621 cgccacgaag gtttctgtat tgctgctctt gttcgcgccc tcttcttcca gatatcccgg
    22681 cacttttttg ccctgggcga agcccgcagt atattgcccg cgtacggttt tttcgcgcac
    22741 gttggagcgg tcaatgcggc gcagagactt cagtactttc actttttcat cgcggatgct
    22801 gtctgcactc aggtcagacg gcggagacat cgcaatcatg caaagaattt gcagcaggtg
    22861 gttctggatc atgtcgcgca tctgaccggc tttatcaaaa tagccccagc gcccttcgat
    22921 ccccacttct tctgccacgg taatctcaac atgatcaatg gtgcgattgt cccagttatt
    22981 cacaaacagg gagttagcaa aacgcagcgc caacaggttc agcaccgttt ctttaccaag
    23041 atagtggtcg atacggtaaa cctggcactc ctcgaagtat tcgccaacct gatcgttgat
    23101 ttcctgcgag gtcgccagcg acgtccccag cggtttctcc atgactacgc gtgccggttt
    23161 agcattcagt tttgcctcac caagcccttt gcaaattgcg ccaaaagtgc tgggcggcat
    23221 ggcaaagtag ttaatggtga tacgattttt ttgatccagc atcgcgccga gacggctgaa
    23281 tgcagcagtg tcattgacat cgagattaca aaaatccaga cgtgcactca gggtgtccca
    23341 taaaccttca tcaatggttt ctttcatgaa agtttcgagc gcctcgcgga caactttggt
    23401 ataagccgct ttatcccagt cagcacgccc tacgccgata atccgggtgt ccgggttgag
    23461 ctgaccggct ttttccagtt gatacaggga aggcagcaat ttacgacgcg caaggtcgcc
    23521 tttcgcgccg aaaatgacca ggtcacaggc ctgggctgtt tgcgttaccg ccatgtcatt
    23581 ctccttaagt taactatccc ggtacttaag ccagggtata cttgtaattt tcttacggtg
    23641 cactgtactg cttttacgag cttgcgaaaa ctgtaaacgc ttatccaccc gtgcgattac
    23701 ggtaaaagcg cgcaaagtgc ggcaaaactg ataaaaaaat catcgttttc cattgggtaa
    23761 aaatctgaca ctgatcatgt tatgaaaaaa aataacaact tttttatctg cttttgtcat
    23821 taacacagca cacaggcgta atatctgaca aaactgcatt tcgatttctt tcagagcgga
    23881 aatcgtcatt acccgtgagt ctctttacat catgaatatg ctggaaaaaa tccagtctca
    23941 gctggaacat ttgagcaaat ctgagcgcaa agttgccgag gtcattctgg cttcgcccga
    24001 taacgcgatc cattcgagta ttgctgctct ggcgctggaa gccaatgtta gcgaaccgac
    24061 ggtgaatcgt ttctgtcgca gcatggacac tcgcggtttt cctgatttta aacttcatct
    24121 ggcacaaagt ctggcgaatg gcactcccta tgttaatcgc aacgtcaatg aagatgacag
    24181 cgttgaatca tacacgggga aaatatttga gtccgcaatg gcaacgctcg atcatgtccg
    24241 tcattcactg gataaatctg ccatcaaccg cgccgtcgat ttgctcactc aggcaaaaaa
    24301 aatcgccttt ttcggattag gctcttcagc cgccgttgcc cacgatgcga tgaataagtt
    24361 ctttcgtttt aatgttccgg tggtgtactc cgatgatatc gtgctgcaac gcatgagttg
    24421 tatgaattgt agcgacggag acgtggtggt gctgatttct cacacgggaa gaacaaaaaa
    24481 tctggtcgag ctggcgcagc tggcacgcga aaacgacgcc atagtgattg ccctcacctc
    24541 tgcgggtacc ccgctcgccc gggaagcaac gctggcaatt accctcgacg taccggaaga
    24601 tactgacatt tatatgccca tggtttctcg acttgcgcag ctgaccgtga tagatgtgct
    24661 ggcgacagga tttactttgc gacgcggtgc aaaattcaga gataacttga agcgggtcaa
    24721 ggaagcgctg aaggaatcgc gttttgataa gcagctactt aatttaagtg acgatcgcta
    24781 aaaacgactg tcactgtcct aatcttatac gacatccgaa tgagattaat ttatcgccat
    24841 cgcggcgtta tttcattcgg atttcatgtt caagcaacac ctggttgttt cagtcaacgg
    24901 agtattacat gtccagaagg cttcgcagaa caaaaatcgt taccacgtta ggcccagcaa
    24961 cagatcgcga taataacctt gaaaaagtta tcgcagcggg tgccaacgtt gtacgtatga
    25021 acttttctca cggctcgcct gaagatcaca aaatgcgcgc ggataaagtt cgtgaaattg
    25081 ccgcaaaact ggggcgtcat gtggctattc tgggtgacct ccaggggccc aaaattcgtg
    25141 tatccacctt taaagaaggc aaagttttcc tcaatattgg ggataaattc ctgctcgacg
    25201 ccaacctggg taaaggtgaa ggcgacaaag aaaaagtcgg tatcgactac aaaggcctgc
    25261 ctgctgacgt cgtgcctggt gacatcctgc tgctggacga tggtcgcgtt cagttaaaag
    25321 tactggaagt tcagggcatg aaagtgttca ccgaagtgac cgtcggtggt cccctctcca
    25381 acaataaagg tatcaacaaa cttggcggcg gtttgtcggc tgaagcgctg accgaaaaag
    25441 acaaagcaga cattaagact gcagcgttaa ttggcgtaga ttacctggct gtctccttcc
    25501 cacgctgcgg cgaagatctg aactatgccc gtcggctggc acgcgatgca ggatgtgatg
    25561 cgaaaattgt tgccaaggtt gaacgtgcgg aagccgtttg cagccaggat gcaatggatg
    25621 acatcatcct cgcctctgac gtggtaatgg ttgctcgcgg cgacctcggt gtggaaattg
    25681 gcgatccgga actggtcggc attcagaaag cgttgatccg tcgtgcgcgt cagcttaacc
    25741 gcgcagtaat cacggcgacc cagatgatgg agtcaatgat tactaacccg atgccgacgc
    25801 gtgcagaagt catggacgta gcaaacgccg ttctggatgg tactgacgct gtgatgcttt
    25861 ctgcagaaac tgctgctggt cagtatccgt cagaaaccgt tgcagccatg gcacgcgttt
    25921 gcctgggcgc ggaaaaaatc ccgagcatca acgtttctaa acaccgtctg gacgttcagt
    25981 tcgacaatgt ggaagaagcc attgccatgt cagcaatgta cgcggctaac cacctgaaag
    26041 gcgttacggc gatcatcacc atgaccgaat cgggtcgtac cgcgctgatg acctcccgta
    26101 tcagctctgg tctgccaatt ttcgccatgt cacgccatga acgtacgctg aacctgactg
    26161 ctctctatcg cggcgttacg ccggtgcact ttgatagcgc taatgacggc gtagcggctg
    26221 ccagcgaagc ggttaatctg ctgcgcgata aaggttactt gatgtctggt gacctggtga
    26281 ttgtcaccca gggcgacgtg atgagtaccg tgggttctac taataccacg cgtattttaa
    26341 cggtagagta agtacgttgc cggatgcggc gaaaacgcct catccggcct acagttcaat
    26401 gataattcaa cagatttcga atcttctgaa gcaaacttga acttatcacc agtcgaaggc
    26461 ctctcctcgc gagaggcatt ttttatttga tgggataaag atctttgcgc ttatacggtt
    26521 ggatttcgcc cggtttgcga gttttcagca attttaatat ccaggtgtat tgttctggtc
    26581 gcggaccaac aaaaatctcg acttcttcat tcatccgccg cgcaatcgta tgatcatccg
    26641 cctctaacag atcatccatc ggtgggcgca cctgaatcgt caaacgatgc gttttgccat
    26701 cataaatcgg aaacagcggt acaacgcgcg cacggcacac tttcatcaaa cgaccaatcg
    26761 caggcaacgt cgctttatag gtggcaaaga aatcaacaaa ttcgctgtgc tctgggccat
    26821 gatcctgatc gggtaaataa tatccccagt agccctgacg taccgactgg atgaatggtt
    26881 taataccatc atttctcgca tgcagacgac cgccaaagcg acgacgcacc gtgttccaga
    26941 cataatcaaa aaccgggttg ccctgattat ggaacatcgc tgccattttc tgcccttgcg
    27001 aggccatcag catggcagga atatcgacgg cccaaccgtg cggcaccaga aaaatcactt
    27061 tctcgttatt acgccgcatc tcttcgataa tctccagccc ttgccagtca acgcgcggct
    27121 gaattttctc cggcccgcgt attgccaact cagccatcat tgccatcgct tgcggcgcgg
    27181 tggcaaacat ctcatctaca atcgcttcgc gttcagcttc actacgttct ggaaagcaga
    27241 gcgacagatt gattaacgca cgacggcgtg agctttttcc cagtcgtccg gcaaaacgtc
    27301 ccagccgtgc cagaatggga tcacggaact ttggcggcgt taaagcgata cccgccatcg
    27361 cggctacgcc cagccatgct ccccagtagc gcgggtggcg aaaggattta tcaaactcag
    27421 gaatgtattc gctattattt tttttcgttt ccatgctttt ccagtttcgg ataaggcaaa
    27481 aatcaatctg gtgatagtgt agcggcgcaa cttgccccgc accaaataaa aaaagccggt
    27541 actgactgcg taccggctgc gaatggatgt taattaatca aaccgtagct gcggcacaat
    27601 ctctttggcc tgcgccagga attcgcgacg atcggagccg gtcagccctt cggtacgcgg
    27661 cagttttgcc gtcagcgggt ttacggcctg ctggtttatc catacttcat agtgcagatg
    27721 cggcccggtt gaacgtccgg tattaccgga aagcgcgata cggtcgccac gtttcacctt
    27781 ctgtcccggt ttcaccagga tcttgcgcaa gtgcatataa cgcgtggtgt agctgcgacc
    27841 atgacgaata gccacataat aacctgctgc gccactgcgt ttggcaacca ccacttcacc
    27901 gtcacccact gaaagcactg gcgtaccttg tggcatggca aaatcaacac ctctgtgtgg
    27961 cgcaacgcga ccggtcaccg gattagtacg acgcgggtta aagttagatg agatacggaa
    28021 ctgtttcgcc gtcgggaatc gcaagaatcc tttcgccaga ccagtaccgt tacgatcgta
    28081 gaatttgcca tcttcagcgc ggattgcgta ataatcttta ccttctgaac gcaaacgtac
    28141 gcccagcagc tggctttgct cacgtttacc atcaagcatt tctcgtgaca ttaacaccgc
    28201 aaattcatcg ccttttttca gtttgcggaa atccatttgc cactgcatgg ctttaatcac
    28261 tgcgctcact tcggcgctgg ttaaaccggc gtttctggcg ctggcaacaa agcttccccc
    28321 gacggtacct ttcagcagat tgttgaccca ctctccttgc tgcatttcgc tggtcatttt
    28381 aaaaccgtta gcggcagtac ggtcataggt tcgggtttca cgacgagaca cttcccaggt
    28441 gaggcgctgc agttcgccgt ccgcggttaa tgtccaggag agttgttgac cgattttcag
    28501 gttacgcaat tctttgtcgg cagcagccag ttgggtgata tcacccatat caataccata
    28561 ctgattgaga atgctgctta gcgtatcgcc agtggaaaca acatattcat gcacgcccgc
    28621 ttcaccggcg attttgtcat ccagttcatc ctggggaatg gcttcatctt cttgtgcagc
    28681 ttgatcaatc ggctcactgg cttcaggtaa gagcgaacga atttcgttct gttccagctc
    28741 aatggttttg acaattggcg tggcatcgcg gtgataaaca tagggccgcc agacagcgac
    28801 ggccagagta agaacggtga gcgaccctaa cataacgcgg tgtggtcgcg gtaaattatt
    28861 aaacgccagg gcgacagagc gggctatctg ttgcacgtaa tcacttcctc attaatctcc
    28921 tttcaggcag ctcgcatact ggttggctaa ttgattcagg aattctgaat agcttgtttt
    28981 acccagtttg atattcgtcc ccaggggatc caacgttccc atacgaacgg atgtccctcg
    29041 tgcgacgctc tcaacgaccg ctggcctgaa ctgtggctca gcaaaaacgc aggttgcttt
    29101 ttgctcaacc aactgtgttc ttatttcatg taaacgctgc gcgccaggtt gaatctcagg
    29161 gttaacggta aaatgaccaa gcggtgtcag tccgaactgt ttttcgaagt agccgtaagc
    29221 atcgtgaaaa acgaaataac ctttcccctt gagcggcgcg agctcgttac caacctgcgt
    29281 ttcggttgag gctaattgtg cctcaaaatc cttcaggttg gcgtcaagtt tggctcgact
    29341 ttgcggcata agttccacta attttccatg gattgcaacc gctgtagccc gcgctatctc
    29401 tggggaaagc caaagatgca tgttgaaatc gccgtgatgg tgatcttcgt cacttttttc
    29461 cgcgtggtcg tgatcatcat catcgtcgtg aatacttttc atcagcagcg gtttcacatc
    29521 ttctagctgc gcaatcgtta cctgtttcgc ttctggtaat ttacttaccg gtttttgcat
    29581 gaacgcttcc atctccgggc caacccaaac gactaagtcc gcgttctgta agcgttttac
    29641 atccgatgga cgcagtgaat aatcatgttc tgaagcgccg tcaggcagta aaacctgcgt
    29701 ttctgttacc ccatcagcaa tggcagaagc gatgaaccca acgggtttaa gcgaagcgac
    29761 aacggcggca tctgcggcct gtgttgcacc gccccagaga gcggcggata atgctgcgaa
    29821 aagaagcgtt tttttatgta acataatgcg accaatcatc gtaatgaata tgagaactgt
    29881 gatattataa catttcatga ctactgcaag actaaaatta acatgacaag tctggtttcc
    29941 ctggaaaatg tctcggtttc ttttggccaa cgccgcgtcc tctctgatgt gtcgctggaa
    30001 cttaaacctg gaaaaatttt gactttactt gggccaaacg gcgcaggtaa gtcgacactg
    30061 gtacgggtag tgctcgggct ggtaacaccc gatgaagggg ttatcaagcg caacggaaaa
    30121 ctgcgcatcg gctatgtacc gcagaagctg tatctcgaca ccacgttgcc actgaccgta
    30181 aaccgttttt tacgcttacg ccctggcaca cataaagaag atattttgcc tgcactgaaa
    30241 cgtgtccagg ccgggcatct gattaacgca ccgatgcaaa agctctcggg tggcgaaacg
    30301 cagcgtgtac tgttagcgcg agcattgtta aatcgaccgc aattattagt gctggatgaa
    30361 cccactcagg gcgtggatgt gaatggtcag gtggcgttat atgaccttat tgaccaactg
    30421 cgtcacgaac tggattgtgg cgttttaatg gtatctcacg atctgcatct ggtaatggca
    30481 aaaaccgatg aagtgctttg cctgaatcac cacatttgtt gttccggcac accggaagtt
    30541 gtttccctgc atccggagtt tatttctatg tttggtcctc gtggtgctga acaactgggt
    30601 atctatcgcc atcatcataa tcatcgtcac gatttacagg gacgaattgt tttgcgtcgg
    30661 ggaaatgatc gctcatgatt gaattattat ttcccggttg gttagccggg atcatgctcg
    30721 cctgtgccgc gggtccgctg ggttcgtttg tagtctggcg tcgtatgtct tatttcggtg
    30781 atacgctggc tcatgcctca ttacttggcg tcgcgtttgg tttgttgctg gacgtgaatc
    30841 cattctatgc ggtgattgca gttacgctgc tgctggcggg cggtctggta tggctggaga
    30901 agcgtccaca gctggcgatc gacacgttat tagggattat ggcgcacagt gccctgtcgc
    30961 tgggcctggt ggtcgttagt ctgatgtcta atattcgtgt tgatttgatg gcttacctgt
    31021 tcggtgattt actggcagtg acgccagaag atctcatctc tattgcgatt ggcgtggtca
    31081 tcgtggtggc tattatgttc tggcaatggc gcaatttgct gtcgatgacg attagcccgg
    31141 atctggcgtt tgttgatggt gtgaaattac agcgcgtgaa attgttgttg atgctggtga
    31201 cggcattgac gattggtgta gcgatgaaat ttgtcggcgc gttgattatt acttcactgc
    31261 tgattattcc tgctgctact gcacgtcgct ttgcccgcac gccggaacag atggctggtg
    31321 tcgctgtttt ggtggggatg gtggcagtga ctggcggttt aaccttttcc gcattttacg
    31381 atacacctgc aggcccgtcg gtggtgctat gtgcggcact gttatttatt atcagtatga
    31441 tgaaaaagca ggccagctaa tctatcgctg aacacatttg tcggatgcgg cgcgagcgcc
    31501 ttatccgacc tatggttcgc tatctctggt atatgcctga taagacgcga atagcggcgc
    31561 atcaggcaac actgccagtg tcggatgcgc cgcgagcgac cgatccgact tacggcattt
    31621 ctggcggcgt gatgccgaag tggttccacg cccgcactgt cgccatacgc ccgcgcggtg
    31681 tacgctgcaa aaagccttgc tgaatcaaat aaggttccag cacatcctca atggtttcac
    31741 gttcttcgcc aatggcagcc gccaggttat ccagacctac cggcccacca aagaacttat
    31801 cgattaccgc cagcaacaat ttgcggtcca tataatcgaa accttcggca tcgacattca
    31861 gcatatccag cgcctgagca gcgatatcag ccgagatggt gccatcgtgc ttcacttcgg
    31921 cgaaatcacg cactcgacgc agcagacggt tagcaatgcg cggcgtaccg cgagcgcgac
    31981 gagcaacttc cagcgcgccg tcatcactca tctcaagccc cataaagcgt gcgctgcgac
    32041 tgacgatata ttgcagatcc ggcacctgat aaaactccag acgctgcaca ataccaaaac
    32101 gatcgcgcaa cggtgatgtc agcgaacctg cgcgcgtggt tgcgccaatc agtgtaaacg
    32161 gcggcaaatc aattttaatg gagcgtgccg ccggaccttc accaatcatg atatccagtt
    32221 ggtagtcttc cattgccgga tacaacacct cttccaccac tggtgaaaga cggtggatct
    32281 catcaataaa cagtacatcg tgtggttcaa ggttagtgag cattgctgcc agatcgcccg
    32341 ccttttccag caccggacca gaagtcgtgc gtaaattaac gcccatttca ttggcgacaa
    32401 tattggcaag cgtagtttta cccaaccccg gaggaccaaa aatcaacaga tgatcgaggg
    32461 catcgccgcg cagtttcgct gctttgatga aaatctccat ctgtgaacga acctgcggtt
    32521 gaccaacata ctcttccagt aatttagggc gaatggcgcg atctgccaca tcttccggca
    32581 aagtggtacc ggcagaaatc agacggtctg cttcaatcat cctttacctc ataacgcggc
    32641 gcgtagggct tcgcgaatta atgtttcact gctggcgtca gggcgagcga ttttgctcac
    32701 catacggctt gcttcttgtg gtttatagcc cagcgccacc agcgcagcaa ccgcttcctg
    32761 ttcagcatcg tcggtcgccg ggctggcagg tgacgtgagt accaggtcgg cggctggcgt
    32821 aaagagatcg ccatgcaaac ctttaaatcg gtctttcatt tcgacaatca agcgttcggc
    32881 ggtttttttg ccaatacccg gcagtttcac cagtgccccc acttcttcac gctcaacggc
    32941 attaacgaac tgctgcgctg acattccgga gaggatcgcc agcgccaact tcgggccgac
    33001 gccgttggtt ttgatcaact ctttgaacaa cgtgcgctct tgtttattgt taaaaccgta
    33061 cagcagttgc gcgtcttcac gcaccacaaa gtgggtgaaa acgatcgctt cctgacccgc
    33121 ttcagggagt tcataaaaac aggtcatcgg catatgcact tcatagccta cgccgcccac
    33181 ttcaattaac accagcgggg gttgtttttc aatgatgatg cctctgagtc tgcctatcac
    33241 atgacgctcc tgcgtaatga atcaaagata atgctgtatg ataaaaaaat gctggataga
    33301 tatccagcga aggatgaaga aaacttgcga ggtgtcccga tgatctgaaa a
//
LOCUS       DAZKJR010000043        33192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKJR010000043 DAZKJR010000000
VERSION     DAZKJR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.043"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            379..1596
                     /gene="alaA"
                     /locus_tag="ACHOWS_002035"
     CDS             379..1596
                     /gene="alaA"
                     /locus_tag="ACHOWS_002035"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFQ0047609.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1680..2279
                     /gene="yfbR"
                     /locus_tag="ACHOWS_002036"
     CDS             1680..2279
                     /gene="yfbR"
                     /locus_tag="ACHOWS_002036"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFQ0047610.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2338..4170)
                     /gene="yfbS"
                     /locus_tag="ACHOWS_002037"
     CDS             complement(2338..4170)
                     /gene="yfbS"
                     /locus_tag="ACHOWS_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFQ0047611.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4257..4907)
                     /gene="hxpA"
                     /locus_tag="ACHOWS_002038"
     CDS             complement(4257..4907)
                     /gene="hxpA"
                     /locus_tag="ACHOWS_002038"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFQ0047612.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4918..5412)
                     /gene="yfbU"
                     /locus_tag="ACHOWS_002039"
     CDS             complement(4918..5412)
                     /gene="yfbU"
                     /locus_tag="ACHOWS_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0047613.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5496..5951)
                     /gene="yfbV"
                     /locus_tag="ACHOWS_002040"
     CDS             complement(5496..5951)
                     /gene="yfbV"
                     /locus_tag="ACHOWS_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFQ0047614.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6289..7491
                     /gene="ackA"
                     /locus_tag="ACHOWS_002041"
     CDS             6289..7491
                     /gene="ackA"
                     /locus_tag="ACHOWS_002041"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFQ0047615.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7566..9710
                     /gene="pta"
                     /locus_tag="ACHOWS_002042"
     CDS             7566..9710
                     /gene="pta"
                     /locus_tag="ACHOWS_002042"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0047616.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9900..11420
                     /gene="yfcC"
                     /locus_tag="ACHOWS_002043"
     CDS             9900..11420
                     /gene="yfcC"
                     /locus_tag="ACHOWS_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0047617.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11453..11995)
                     /gene="yfcD"
                     /locus_tag="ACHOWS_002044"
     CDS             complement(11453..11995)
                     /gene="yfcD"
                     /locus_tag="ACHOWS_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFQ0047618.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12053..12604)
                     /gene="yfcE"
                     /locus_tag="ACHOWS_002045"
     CDS             complement(12053..12604)
                     /gene="yfcE"
                     /locus_tag="ACHOWS_002045"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFQ0047619.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12660..13304)
                     /gene="yfcF"
                     /locus_tag="ACHOWS_002046"
     CDS             complement(12660..13304)
                     /gene="yfcF"
                     /locus_tag="ACHOWS_002046"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFQ0047620.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13440..14087
                     /gene="yfcG"
                     /locus_tag="ACHOWS_002047"
     CDS             13440..14087
                     /gene="yfcG"
                     /locus_tag="ACHOWS_002047"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFQ0047621.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14144..14506
                     /gene="folX"
                     /locus_tag="ACHOWS_002048"
     CDS             14144..14506
                     /gene="folX"
                     /locus_tag="ACHOWS_002048"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFQ0047622.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14527..15420
                     /gene="yfcH"
                     /locus_tag="ACHOWS_002049"
     CDS             14527..15420
                     /gene="yfcH"
                     /locus_tag="ACHOWS_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFQ0047623.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VALWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCAHWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15468..16370)
                     /gene="rpnB"
                     /locus_tag="ACHOWS_002050"
     CDS             complement(15468..16370)
                     /gene="rpnB"
                     /locus_tag="ACHOWS_002050"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFQ0047624.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16567..17340)
                     /gene="hisP"
                     /locus_tag="ACHOWS_002051"
     CDS             complement(16567..17340)
                     /gene="hisP"
                     /locus_tag="ACHOWS_002051"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFQ0047625.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17348..18064)
                     /gene="hisM"
                     /locus_tag="ACHOWS_002052"
     CDS             complement(17348..18064)
                     /gene="hisM"
                     /locus_tag="ACHOWS_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0047626.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18061..18747)
                     /gene="hisQ"
                     /locus_tag="ACHOWS_002053"
     CDS             complement(18061..18747)
                     /gene="hisQ"
                     /locus_tag="ACHOWS_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFQ0047627.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18837..19619)
                     /gene="hisJ"
                     /locus_tag="ACHOWS_002054"
     CDS             complement(18837..19619)
                     /gene="hisJ"
                     /locus_tag="ACHOWS_002054"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFQ0047628.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19840..20622)
                     /gene="argT"
                     /locus_tag="ACHOWS_002055"
     CDS             complement(19840..20622)
                     /gene="argT"
                     /locus_tag="ACHOWS_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFQ0047629.1"
                     /translation="MKKSILALSLLVGLSAVASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20888..21457)
                     /gene="ubiX"
                     /locus_tag="ACHOWS_002056"
     CDS             complement(20888..21457)
                     /gene="ubiX"
                     /locus_tag="ACHOWS_002056"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFQ0047630.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21552..23069)
                     /gene="purF"
                     /locus_tag="ACHOWS_002057"
     CDS             complement(21552..23069)
                     /gene="purF"
                     /locus_tag="ACHOWS_002057"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0047631.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23106..23594)
                     /gene="cvpA"
                     /locus_tag="ACHOWS_002058"
     CDS             complement(23106..23594)
                     /gene="cvpA"
                     /locus_tag="ACHOWS_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFQ0047632.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23853..24503)
                     /gene="dedD"
                     /locus_tag="ACHOWS_002059"
     CDS             complement(23853..24503)
                     /gene="dedD"
                     /locus_tag="ACHOWS_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFQ0047633.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAVNNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPASKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(24493..25761)
                     /gene="folC"
                     /locus_tag="ACHOWS_002060"
     CDS             complement(24493..25761)
                     /gene="folC"
                     /locus_tag="ACHOWS_002060"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFQ0047634.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYTSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25831..26745)
                     /gene="accD"
                     /locus_tag="ACHOWS_002061"
     CDS             complement(25831..26745)
                     /gene="accD"
                     /locus_tag="ACHOWS_002061"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0047635.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26901..27560)
                     /gene="dedA"
                     /locus_tag="ACHOWS_002062"
     CDS             complement(26901..27560)
                     /gene="dedA"
                     /locus_tag="ACHOWS_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFQ0047636.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27643..28455)
                     /gene="truA"
                     /locus_tag="ACHOWS_002063"
     CDS             complement(27643..28455)
                     /gene="truA"
                     /locus_tag="ACHOWS_002063"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0047637.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28455..29468)
                     /gene="usg"
                     /locus_tag="ACHOWS_002064"
     CDS             complement(28455..29468)
                     /gene="usg"
                     /locus_tag="ACHOWS_002064"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0047638.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29534..30691)
                     /gene="pdxB"
                     /locus_tag="ACHOWS_002065"
     CDS             complement(29534..30691)
                     /gene="pdxB"
                     /locus_tag="ACHOWS_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFQ0047639.1"
                     /translation="MSRTEKLLTILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLAD
                     ADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDDTWLKQAGIGFSAAPGCNAIAV
                     VEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRG
                     DEGDFRSLDELVQHADILTFHTPLFKDGPYKTRHLADEKLIRSLKPGAILINACRGAV
                     VDNTALLTCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQ
                     VFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLR
                     KVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            complement(30851..31855)
                     /locus_tag="ACHOWS_002066"
     CDS             complement(30851..31855)
                     /locus_tag="ACHOWS_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0047640.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFNKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWVYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSRRIADGLKPSSANRYMNRLSGMFTALKKIGLWDAEHPVRGISVLYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWGEASKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTSETGGLFDVKYREFCLVLKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQRILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHSVSTGE
                     "
     gene            complement(31952..32281)
                     /locus_tag="ACHOWS_002067"
     CDS             complement(31952..32281)
                     /locus_tag="ACHOWS_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0047641.1"
                     /translation="MPDMASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEV
                     RGKQVTEGALLKVTKHPKFKKYAYWLSTDETMPEAGQIAPALSLDGSCNLEGNQDSTE
                     TTRKLPR"
     gene            32386..32673
                     /locus_tag="ACHOWS_002068"
     CDS             32386..32673
                     /locus_tag="ACHOWS_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFQ0047642.1"
                     /translation="MRESELEGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNAGMRKAFFNQPKEQRDAWLLWLGL"
     gene            32869..>33192
                     /locus_tag="ACHOWS_002069"
     CDS             32869..>33192
                     /locus_tag="ACHOWS_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFQ0047643.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHP"
BASE COUNT         8316 a         9035 c         7994 g         7847 t
ORIGIN      
        1 ttaacaatag tttcacacct tgcacccatt acgacacctc ggaaatcatc acgctgttaa
       61 actctccgta atgcggaaat atcatcatca cgattcatta gtgaattctt aaacgaggca
      121 ttttcaaaca attatcttac agataaaaaa accacagtca caattaagaa tcagaacaat
      181 tcactacata acattaatta ctaagcatat acacctcatt attttgccat tattaagtta
      241 ttaacagcac aatcgagtct tcccctctgg caaaatctta ttctgcagac cttcaaaacg
      301 ccgtcctggg ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag
      361 caaaaaaggt caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt
      421 tacgacatcc gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag
      481 gtactgaaac tgaacatcgg caaccctgcc ccgttcggtt ttgacgcgcc agatgaaatc
      541 ctcgttgacg tgattcgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt
      601 tactccgcgc gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc
      661 gtggaagata tttacatcgg aaatggtgta tcggagctta tcgttcaggc aatgcaggca
      721 ttgctgaaca gcggcgacga aatgttggtt cctgcacctg attacccact atggaccgcg
      781 gcggtttcgc tctccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg
      841 ttcccggacc ttgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gatcgttatt
      901 atcaacccaa ataacccaac cggcgcggta tattccaaag agcttttaat ggagatagtg
      961 gagattgcac gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc
     1021 tacgacgacg ctgagcatca ctcaattgcg ccgctggcac ctgatctgct gaccattacc
     1081 tttaacggcc tgtcgaaaac ataccgcgtt gcaggcttcc gtcaggggtg gatggtgttg
     1141 aacgggccga aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg
     1201 cgcctgtgtg ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg cggttatcag
     1261 agcatcagtg aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcatgg
     1321 gaattgatca atgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtacatg
     1381 ttcccgaaaa tcgacgccaa acgctttaat attcacgacg atcagaaaat ggtgctggat
     1441 ttcctgttgc aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg
     1501 gatcacttcc gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag
     1561 ttcgcgcgtt tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg
     1621 catccggcag tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta
     1681 tgaaacagag ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc
     1741 taatgcgcaa tgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg
     1801 cccatgcgct ggcagctatc aaaaatcgta aatttggcgg taatgtcaac gccgaacgta
     1861 tcgctttact ggcgatgtac cacgatgcct cagaagtgct taccggcgat ctccctaccc
     1921 cggtgaaata cttcaattcg caaatcgctc aggaatacaa ggcgattgaa aaaatcgctc
     1981 agcaaaaact ggtcgatatg gttccggaag agttgcggga tatctttgcg ccgttaattg
     2041 acgagcatgc atatagtgat gaagaaaaat cactggtgaa acaggcagat gcactatgtg
     2101 catatctgaa atgtctggaa gaactcgcgg cgggaaataa tgaattcttg ctggcaaaaa
     2161 cgcgactgga agcgacgctt gaagcgcgcc gcagccagga gatggactac ttcatggaag
     2221 tctttgttcc cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag
     2281 cagccggagt ctgcgtcgca tcaggcaata agcgccggat gcgacatcag gctcttgtca
     2341 aaacggaaac agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg
     2401 tacccccaac ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagtgtgtt
     2461 aacaggtgaa gaaaccggcg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc
     2521 gaatgggtaa ggcgacaccc ccatcgtttt agcagcagcc agcgcaatcg gagccatcaa
     2581 caccgccgtc gcggtattag aaataaatag cccaataacc gccgacaaga caaacaaaca
     2641 acccagcatc atatgtggcc cgtaaccgcc gccaatgtcc atcagccctt tcaccgccag
     2701 cgcaacacct cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat
     2761 aatgctcggc cagtgtatgg atttataggc gctttcagca tcgatacagc ggaatttccc
     2821 catcagcagg caggcgatga tagcggcaac aggattagga atttcatcgg tcagcattaa
     2881 cgccaccatt agcaccagac agaaaatggc atggggtgcc tgactgtgcg cgggcgatgc
     2941 ttcactcacc tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat
     3001 accgatcagt ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc
     3061 cgccagcgaa ccttccagcg ccacgccatt gcgctttagc cccactacat tcaatccgta
     3121 gcgggtacga aaaccaattt cgcgcaccga tttaccaata agttctgact caggaattaa
     3181 tgaaatctct gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac
     3241 catcggctcc agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat
     3301 atcaataagc aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac
     3361 gcgacgaaaa cgccgccagc gttcaacacc gatgacgtta gcaccataac gttcacgtaa
     3421 tttgagatcg tccagtcgtt gaccaatcat tggcgatccg gggcgaatag ccagacgtcg
     3481 cgcacgcccg gtcagtcgat attcacggat aagatcgcga aaggttcggc gcgtccagcc
     3541 ttcgcgttgt ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa
     3601 aatacccagc accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg
     3661 atagccttca cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt
     3721 catcatgccg cttatcagcc cggcaaaact taacggcatc atcagacgcg acggcgacgt
     3781 ttgcatacgc atggcaacgc ttaacaccac gggaataaag atagcgacaa cgccggttga
     3841 actcataaac gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc
     3901 actgttgccc gccactttga ccagccatgt ccccattaca gtggcaacac cggtacggac
     3961 caaaccatcg ccaataatga acaaggcggc aatcaggaca acgttaggat cggaaaagcc
     4021 ggaaaatact tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa
     4081 agcgaccgca tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa
     4141 tgaaagaacc caaatcaatt caccgttcac aacttatcct tgttaattga gggggataac
     4201 ttgattctgc cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac
     4261 tgaataataa catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg
     4321 accaaatcga cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg
     4381 caacccgccg ccaggccaga aagcacgcca gcgggagcat cttccaccac tacacactcc
     4441 tgcggcgcaa gccccagcag ctgcgcgcct agcagatacg catcaggttc tggttttccg
     4501 cgcttcactc gctcagcggt cacaaacacc tctggcgcgg gaagcccagc tattttatgg
     4561 cgcgctcgcg ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta
     4621 ttcaaatgat tgagtaaggc gattgccccc ggaagtgcgg taataccctc ggtttccgtg
     4681 gcctcgattt gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc
     4741 ataaaatggc gcaaagaggt gatcgcctgt ttaccgtgaa taaaagccag cacctcttcc
     4801 ggcgctaacc catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa
     4861 tccaccagcg ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca
     4921 ggcgttgata atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg
     4981 ccacacatta agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccgtg
     5041 agttccggca tcaaaatggg tatagcgtcc ttccacatta accataaagc ggacataacc
     5101 gaggtaacgt gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat
     5161 ggattgctgg tcctgcaaat tagaccagga aacatgcaac gcatgataca tctccataat
     5221 gtcgatgata gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccagttcacg
     5281 catctgtaat ccataaccac gctcaatgat tgtttgcagg cgacggtaac gttcagcatt
     5341 tgccggatcg agcatagtca tcatcttgta ctggttagac aaaataagac gttgcgcgtt
     5401 ggtcatttcc attgttgact cctgtatcac tctactacgg tgaaaaaaaa agaaggctga
     5461 gtatgccttc ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta
     5521 tccagttgtt tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct
     5581 tcaacgggtg ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag
     5641 ttaaggattg cagggggtaa tggcgttaca gaacgtttgc ccagccacca caatccctgc
     5701 atgggtaaac ttaaggcgaa cagcgcagtg gcaactgccg gcccaagctg accgcccagg
     5761 gcaatctgcc agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc
     5821 gtcatcttga taacgcgatt ttcgacaaag accggggcaa ggcgtttttc cagcggccac
     5881 gtctttgagt aatgctgtcc ccggcgaaac aagctaaaaa aattaacaga acgattatcc
     5941 ggcgttgaca tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca
     6001 caactcagcg ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt
     6061 tagcagcctg aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat
     6121 gccattggct gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa
     6181 aaaaaatttg cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct
     6241 atggctccct gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag
     6301 ttagtactgg ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta
     6361 aatggtgaag agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc
     6421 aaatggaaaa tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc
     6481 gaagcgctca actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag
     6541 ctgactgcta tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg
     6601 atcgatgagt ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac
     6661 ccggctcacc tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa
     6721 aacgttgctg tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac
     6781 gccctgccgt acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc
     6841 agccacttct atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg
     6901 aacatcatca cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa
     6961 tgcgttgaca cctctatggg cctgaccccg ctggaaggtc tggtcatggg tacccgttct
     7021 ggtgatatcg atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac
     7081 gcaatcaaca aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc
     7141 gactgccgct atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac
     7201 gtttactgcc accgcctggc gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt
     7261 ctggacgctg ttgtattcac cggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg
     7321 tctctgggca aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca
     7381 cgtttcggca aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca
     7441 accaacgaag aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc
     7501 gccagctcag ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat
     7561 aaaccgtgtc ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg
     7621 tcagccttgg cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac
     7681 ctatcgctca gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg
     7741 cgaactcttc caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc
     7801 tttccagcaa tcagaaagat gtgctgatgg aagagatcgt cgcaaactac cacgctaaca
     7861 ccaaagacgc tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag caccagtttg
     7921 cccagtctct gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt
     7981 ctcagggcac tgacaccccg gaacagctga aagagcgtat tgaactgacc cgcaacagct
     8041 tcggcggtgc caaaaacacc aacatcaccg gcgttatcgt taacaaactg aatgcaccgg
     8101 ttgatgaaca gggtcgtact cgcccggatc tgtccgagat tttcgacgac tcttccaaag
     8161 ctaaagtaaa caatgttgat ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg
     8221 gcgcggtgcc gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc
     8281 tgaatgcaac catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt
     8341 tctgcgcacg cagcattcca cacatgctgg agcacttccg tgccggttct ctgctggtga
     8401 cttccgcaga ccgtcctgac gtgctggttg ccgcgtgcct ggctgccatg aacggcgtag
     8461 aaatcggtgc cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt
     8521 gcgaacgtgc tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga
     8581 cttctctgag cctgcagagc ttcaacctgg aagttccggt tgacgatcac gagcgtatcg
     8641 agaaagttca ggaatacgtt gctaactaca tcaacgctga ctggatcgat tctctgactg
     8701 ccacttctga gcgcagccgt cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac
     8761 ttgcgcgcaa agcgggcaaa cgtatcgttc tgccggaagg tgacgaaccg cgtaccgtta
     8821 aggcagccgc tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg
     8881 cagagatcaa ccgtgttgca gcgtctcagg gtgtagaact gggtgcaggc attgaaatcg
     8941 ttgatccaga agtggttcgc gaaagctatg ttggtcgtct ggtcgagctg cgtaagaaca
     9001 aaggcatgac cgaaaccgtt gcccgcgaac agctggaaga taacgtggtt ctcggtacgc
     9061 tgatgctgga acaggatgaa gttgatggtc tggtttccgg tgctgttcac actaccgcaa
     9121 acaccatccg tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt
     9181 ccgtgttctt catgctgctg ccggaacagg tttacgttta cggtgactgt gcgatcaacc
     9241 cggatccgac cgcagaacag ctggcagaaa tcgcgattca gtccgcagat tccgctgcgg
     9301 ccttcggtat cgaaccgcgc gttgctatgc tctcctactc caccgggact tctggtgctg
     9361 gtagcgacgt agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc
     9421 tgatgatcga cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca
     9481 aagcaccgaa ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca
     9541 ccggtaacac cacctacaaa gcggtacagc gttctgctga cctgatctct atcggaccga
     9601 tgctgcaggg tatgcgcaag ccggttaacg acctgtcccg tggcgcactg gttgacgata
     9661 tcgtctacac catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc
     9721 atccgcagct ttacgctgcg gatatctgaa ccggaaatag tcactatttc cggtttttta
     9781 ttctcttaat ttgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa
     9841 ttgcgccatc tcaatttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta
     9901 tgtccgcaat cactgaatct aaaccaacaa gaagatgggc aatgcccgat acgttggtga
     9961 ttatcttttt tgttgccatt ttaaccagcc ttgccacctg ggtagttccg gtgggcatgt
    10021 ttgacagtca ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag
    10081 atccacactc atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac
    10141 agctgttcac gacgggcgat gaacgcccgg gtctgatgaa cttcccgttt gaagggttaa
    10201 cctcaggatc gaaatacggg acagccgttg gcatcatcat gtttatgctg gtgattggcg
    10261 gcgcgtttgg cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc
    10321 gccatacccg cggaaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg
    10381 gcggcgcaat atttggtatg ggagaagagg ccgtcgcctt tgccattatt atcgcaccgc
    10441 taatggtccg gctgggctat gacagtatta ccaccgtcct ggtgacctat atcgccacgc
    10501 aaatcggttt tgccagttcg tggatgaacc cgttttgtgt ggttgttgct caggggattg
    10561 ccggcgttcc ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt atcgccaccc
    10621 tgattggcct gatctttacc atggtgtacg cctcacgagt gaaaaagaat cctcttctgt
    10681 cacgcgtgca tgagtccgac cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc
    10741 cgtttacctt tggtgactgg ctggtattga ttgtcctgac cgccgtaatg gtctgggtga
    10801 tttggggcgt gatcgttaat gcctggttta ttccagaaat tgccagccag ttcttcacca
    10861 tgggtctggt gattggcatc atcggcgttg ttttccgcct taacggcatg acggttaata
    10921 ccatggcttc atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg
    10981 gtttcgccaa agggattttg ctgctggtcg gtaatggtga agcgggtgat gccagcgtgt
    11041 taaataccat cctcaacagc attgccaatg ccattagcgg tctggataac gcggtcgcgg
    11101 cctggtttat gttgctcttc caggcggtat ttaatttctt cgtgacatct ggttctggtc
    11161 aggcggcgtt aaccatgccg ttactggcac cgcttggcga tctggtcggt gtaaaccgtc
    11221 aggttaccgt tctggcgttc cagtttggtg atggcttcag ccacatcatt tacccgacct
    11281 cagcttcgtt aatggcaacg ctcggtgttt gccgggtgga cttccgtaac tggctgaagg
    11341 tgggcgcgac actgcttgga ctgctgttta ttatgtccag cgtcgtggtg atcggcgctc
    11401 agttgatggg ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg
    11461 ccgtttcagt ctctacggct tcatttttgg cattgcgctt catccacaac gccagcgctt
    11521 tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc
    11581 aaacttcgct gacttcatct tcctgtaggg cgaagggacc gtgagagacg cagctgaaca
    11641 acgcgcccca gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg
    11701 ggacaccggc aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct
    11761 catcggcctg gactacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg
    11821 tctcggtacg acgctggacc agaattttgc ccatgccatc atgcacgacg atgtaagttg
    11881 cacgatgacg cagacactgt gcccgcattt gttcccggct ggcttgtgca atgacttcgt
    11941 tctcttcatt gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca
    12001 ggaaaccttc tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat
    12061 taatcgcgac ctgcgcaatg atactttgat cattgagtgc gattacacta agtacgtcat
    12121 tatccagcat gccataactc gccggattac cgcctttagg aatactcacc gagccggggt
    12181 tgaagtggaa aatctccccc cgctgttctg ccactggcag atgggtatga ccgtacacca
    12241 gcacatcgtt ctggtttaaa gcaggtaggt tttccgggcc aaacagatga ccatgcgtca
    12301 aaaacagacg ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca
    12361 aaagcatttg atccacttcg ctgtcgcaat tgccgcgcac agcgataacc ttatgtgcca
    12421 cttcattaag ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa gcattacgcg
    12481 ggccgtgatt caacacgtcg ccaaggatca ccagccactg ggcaccactt tgggcaaaca
    12541 actccagaac acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt
    12601 tcatcactca ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat
    12661 cagccagatt gcttcgccga aagagcaata aaacgctgga cagacgctcg ctgccactgg
    12721 aatgttgcat aatccaccag gcgttctggc acctcatcgc catgtagtac caggcggtta
    12781 atcattagcg ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc
    12841 agtgctaaca aatgttctgc catcgcgaac agtttctctg cactggcttt tccctccgcc
    12901 gttaatggcg cttttttcgc acccgcaaaa acgacatctg tcgaacgttc ttcgcggatg
    12961 ggcatcagat cgctacgcag ccaggcctga atctgtcgtg cacgcgcacg attttctaaa
    13021 tcaagcggat aaatgcgttc ccaggtcggt ggcgcaaatc gatcttccag atactccgca
    13081 atggcagaag attcactcaa ctcaaaatca tcgatttgta ataacggcac acggcgtgtc
    13141 tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc tgtcgaggtc gatggtcttg
    13201 atatgaaacg acaggccttt ttcctgcaac gccacccagg cggataacac ataaggggag
    13261 aaaaaatggg catctgacca aagcgtgata gcgggtttac tcattatgac ctcggcaata
    13321 aagggggctg gctatcaaac tatagagcct ttttgtcact gtcacgtgac gaatactcac
    13381 gcaagtcgcc gcgctttcta tacttgtggt gttcagatga ttagacaacc ggagctgcaa
    13441 tgatcgatct ctatttcgcc ccgacaccaa atggtcacaa aattacgctg tttctcgaag
    13501 aagcagggct ggattatcgc ttgataaagg tagacctggg gaaaggcggt cagtttcgcc
    13561 cggaattttt gcacatttcg cctaacaaca aaattccggc aattgttgat cattctccag
    13621 ccgatggcgg cgaaccgcta agcctctttg agtctggtgc cattttgttg tatctggctg
    13681 agaaaacagg actctttttg agtcatgaaa cgcgtgagcg cgccgccaca ttacagtggt
    13741 tattctggca ggtaggcgga ctggggccga tgcttgggca aaatcatcat tttaatcacg
    13801 cagcctccca aaccattcct tacgctattg aacgttatca ggttgaaact cagcgtctgt
    13861 accatgtact gaacaagcgg ctggaaaact cgccctggct gggtggcgag aactacagca
    13921 ttgcggatat tgcctgctgg ccgtgggtta atgcctggac tcgccagcga attgacctcg
    13981 caatgtatcc ggcagtcaag aactggcatg agcggatccg ttcgcgccct gccaccggtc
    14041 aggcactgtt aaaagcacaa ctcggtgatg agcgttcgga tagttaacag aaacaggttc
    14101 tcgtgtatta tttcatccta agaaaaacaa cggagaacct gcaatggcac aacctgccgc
    14161 tattattcgt ataaagaacc ttcgtttacg tacgtttatc ggaattaagg aagaagaaat
    14221 taacaaccgt caggatattg ttatcaatgt gacgattcac taccccgccg ataaagcgcg
    14281 caccagcgaa gatatcaacg atgcgctgaa ttatcgcacc gtgacgaaaa acattattca
    14341 gcatgtagag aataaccgtt tctctttgct ggaaaaatta actcaggatg tgctcgatat
    14401 cgcacgtgaa catcactggg tgacgtatgc tgaagtggag atcgataaac tgcacgcgct
    14461 gcgctacgcc gattcggtat ccatgacctt aagctggcag cgttaaccgc catatcggga
    14521 ggctgcatga agatagtgat caccggaggg acaggattaa ttggtcgcca tttgattcca
    14581 cgtttgctgg agctgggcca tcaaatcacg gtaatgacgc gtaacccgca gaaagccagt
    14641 tctgttctcg gccctcgggt ggcactatgg caagggcttg ccgatcaaag caacctcaac
    14701 ggcgttgatg cggtaatcaa cctggccgga gaaccgattg ctgataaacg ctggactcac
    14761 gagcaaaaag agcgtctctg ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg
    14821 attaatgcca gcgacacgcc gccgtcggta ctcatttccg gctcggcaac gggctattat
    14881 ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga atttactcat
    14941 aaactctgcg cccactggga agaaattgcc tgccgggcgc aaagtgacaa aacgcgagtg
    15001 tgcctgctgc gtaccggcgt agtgctggca ccggatggcg gtattctcgg taaaatgctg
    15061 ccgccgtttc gtcttggcct gggcgggccg attggttccg gtcggcagta tctggcctgg
    15121 attcatattg atgatatggt caacggcatt ctctggctgc tggataacga gctgcgcggg
    15181 ccatttaata tggtttcgcc ctacccggta cgcaatgaac aatttgccca tgcgctcggt
    15241 catgctctgc atcgcccggc tattttgcgc gtccccgcaa ccgccattcg gctgttaatg
    15301 ggcgaatctt cagtactggt attaggtgga caacgcgcgc tgcctaaacg gctggaagaa
    15361 gcgggttttg cgtttcgttg gtacgattta gaagaggcgc tggcggatgt cgttcgctga
    15421 tgtggtttac tgcaaacatc cgccagttaa ctcccggtgt tacagggtta gtggctttgc
    15481 gcgataagat cgtctggtga aagtcgggtc accatcataa ctaactctcg gtctaaacct
    15541 ctctccagca tctcctgagc aatacgcagg gcttcttcgc gtttgccaag aattaatcca
    15601 tcattccgcc cttcttcacg taatctgtca gcaatagtca tcagtttctc cttttcttgt
    15661 ggtgcgcgtt ccgctatctc accaataaat gcacggaaac gctgggcatc ccccgtttgt
    15721 aatacgtaat taaacagggc ttttagctgt ctgtcattag tgttccctgt aactagcagc
    15781 gaaacaattt ggtcgactaa tcccaacaga tcgcgctgac gaatatgttt ctgaattaac
    15841 tccaacagcg ccattttgcg atgttgcata atctcgtcat ccggcaccac ggtaatatct
    15901 accagcggaa aagccgatga ataaattttg cgggctatag caggctcggc aaattcatca
    15961 agccagcaaa gtgaataagg ataaggactt ctgcaaccat gataaaacag catcgggatc
    16021 accaatggaa gctctttata gcctgcatca agatggtttt gcatcgccgc aatggaatag
    16081 cgcatcatgc gaaaagccat taattcttcc ggcttacttt ggtgctctat cactacataa
    16141 atataacctg ctccctcctg cgttttcaca gaccacaaga ggtcggaata atattgccgc
    16201 aggtcttcat caataaaact gtttggttcc agttttagcg ttgttaaatc acacagtttg
    16261 cgcagcggcg cgggaagatg aatatcaata aaatcccgcg cggtgtctgg atggcgtaaa
    16321 aaagatttaa ataccgcatc atgcggcgtg gaagttgtcg atattgtcat ggcgttccgt
    16381 cacccctcaa atttaagatg acgcgacaat aaccacgatt aatacccaat gcagcaggca
    16441 gtttttatct ttggacagcg aattcagaac tatttttagc ggactgcaac ttttacattt
    16501 cagcggaagc tgactcgcaa attccccttg aaaatactcc ggcagcgtta gccaccggag
    16561 ggggaattat ttcagcgatc ccttaaggaa ctgttgcaga cgagggcttt gcgggttacc
    16621 aaataactgt tctggcgcgc cctcttcttc tattttcccc tgatggagga aaatgacgtg
    16681 agtagaaaca tggcgagcaa agcccatttc gtgagtcact accaccatgg ttttcccctc
    16741 ttctgccagt tgctgcataa tacgcaacac ttcgcctacc agttcaggat cgagcgccga
    16801 ggtaggttca tcaaacagta aaacttccgg ttccatcgcc agcgcccgcg cgatagaaac
    16861 acgctgttgc tgaccaccgg acaggtgcac cgggtatttc ccctgcgcac gttcgtctat
    16921 cccgactttt gccagatatt tcaccgcccg ctcgcgcgct tcctgcttgc tcaggcccaa
    16981 cacctgaatc ggcgcttcca tgacgttttc cagcaccgtc atatggctcc agagattgaa
    17041 gtgctggaat accatcgtca ggcgtgtgcg cagtaagcgc agctgatttt tatcggcgac
    17101 tttgagttga ccatctttgt cgcgcaccag attgatcgtc tggccattga ccacgatcga
    17161 tccttcgctc ggtttttcga ggaagttaat gcagcgcaga aaggtacttt tccccgatcc
    17221 cgacgatccg atgatgctaa ttacatctcc ggcattcgct tgcagtgaaa cccctttcag
    17281 cacttcgtgt tcgccgtagc gtttatgcaa atcgataacg tttaatttat tctcggacat
    17341 cgtattctca gtgcgttgaa gaaggtttca catgctgcaa ccagcgtttt tccgctctgc
    17401 gaaagaggct gatcaggaca taagagatga ttaaatagag caccgcggca atgccgaagg
    17461 cggtaaaagg ttgatatgtg gcggcgttaa tatcgcgggc aattttcagc agatccggca
    17521 ccgtggcagt aaatgccagc gcagtagagt gcagcatcag gatcacttcg ttgctgtacg
    17581 ccggtaacgc aatacgcagc gcagaaggca aaataatgca gcgatacatt ttaaaggtcg
    17641 agaagccata ggctctggcg gcttcaattt ccccatgcgg cactgaacgg attgccccgg
    17701 caaaaatctc ggtagtgtaa gcgcaggtgt taagcgtcag cgccagcacg gtacagttca
    17761 gaccactgcg aaaaaaggcg ttaaggaact cggttccctt aacaatctcc agcgtgtaca
    17821 tgccggaata gaacaccagc aactgaacat acagcggcgt accgcgaaaa atataggtaa
    17881 ataaccagat tggaaactgg atgtatttat tacttgagac gcgaccaatc gccagaaaca
    17941 gcgccagaac tccgcctatc actaccgaca aaataagcag ccacagggtg atcgccacgc
    18001 cagtaaagcg ataaccgtcg gtccacagca gtggtttcca gtattcatgt aagatttcga
    18061 tcacaggtca gccctcttca cacccacgga gtagcggcgc tcaaggaaca gcagcacacc
    18121 attggaaaca gtggtgaaaa ccaggtaaat cacgccacag acgatggcga aatagaacgg
    18181 ttcccaggta cttttgcctg ccagctgagt ggctttgacc acatcttcca ggccgagtaa
    18241 cgaaacgagt gcggtagatt tgaggatcac ctgccagttg ttgccaatgc ctggcagggc
    18301 gtaacgcatc atcgccggaa acatgatccg tcgaaacact tgcccacgag taaaaccgaa
    18361 cgccgtcgcc gcctctatgt gtcctttcgg cactgccata aacgcgccac gaaacgtttc
    18421 ggtaaaataa gcaccgtaga taaaaccgag agtgataata cctgcgacca tcggatcaat
    18481 atcaatctgc ccgacgccca tcgcctccgt caccgtgttt agcgcaatct gcaaaccgta
    18541 gaaaatcagc agcatcaaca ccaagtccgg cacgcctcga atcagcgtgg tgtacccttc
    18601 gaaaatcagc cccgaaagcc gattttgcga gagcttaccg ccagcgccaa ttaaaccgat
    18661 aattacagcg agcactacag agctgatagc cagctccagc gtgacgagcg caccctgtaa
    18721 aataacacct gaaaacccat acaacatgct gcctgtcctg tcgtgtgtgg tgaattaccg
    18781 cctcttgtct ccctccggtt acccgaaggg agcggacttt ctttcaccga tggcgattaa
    18841 ccaccataaa catcaaaatc gaagtacttt ttcgctaatt tctcgtaagt accgtcagcg
    18901 cgcatttcgg caaaggcttt gttcagtgcc tcgcgcagtt cgttatcttc tttacgcagg
    18961 cccatgccgg tccctacgcc aaacagtttt tcatctttaa cagacggacc accgaatttg
    19021 taatctttac cgacgggttg tttgaggaaa ccttcgctgg cagcgacctc atcctggaac
    19081 gcggcatcaa tacgtccggc agtcaggtca gaataaatgt tgtcctgccc ctgataagaa
    19141 acgatttcaa tgccttttgg tgcccaatgt tcgttaccga acgtctcctg ggtggtgccc
    19201 tgcaatacgc ctacccgttt gcctttcagc gactcgactg tcggctgaat gtcggaattt
    19261 ttcgccacca ccagacgaga atcggcagcg tacagtttgt cggtgaaggc aatttcttgc
    19321 tgacgttttt ccgtaatgga aagcgatgac atgatggcgt caatcttctt cgcttttaag
    19381 gacgggatta acgcatccag tgggttttcg acaaacgtac attgcgtatt gatgcgtttg
    19441 cacaactcct tcgccagatc gatatcgaag ccaaccagtt cgccttgtga attttttgat
    19501 tcaaatggcg cataggtcgg atcggtaccg atgcggatgt tttgcggaat cgcagcaaac
    19561 gccgcagttg cgctggagaa ggccagaacc agagagagcg atagcaccag ttttttcata
    19621 atgtcctcaa ctgacagtct ttttataggg aatatttaca ggtttgttac acttatcgtg
    19681 ccataaaatt gaccatcaag gcaaaatatc ccgccccgtt agacgtatta tttttcatta
    19741 aaaatgctta agtatgcatt tcaatgcacc acgatagtgc aacaaacctg gcgtgcacca
    19801 tgatgatcat tccatcaggt acagtttccc tgcgacgtat cagtcaccgt agacattaaa
    19861 gtcgaaatac tttttcgcca tcttgtcgta ggtaccgtcc tgacgcagct cgccaagcgc
    19921 cttattaaac gccgccgtca gttcagcatc atctttacgt agccctacac cggtaccgtc
    19981 accgaagtat tttttgtctt tcactgatga gccagcaaag gcgaaatctt taccagcagg
    20041 ttgcttgagg aaaccttcgc tggccgccac ttcatcctgc agtgcggcat ccagacgtcc
    20101 tgcagccaga tcggaataga ccaaatcctg gttggcatag gccaccacat ccacaccttt
    20161 gctacgccag gtctcgttag cgtaagcttc ctgggttgat ccctgcagca cacccacatg
    20221 tttacctttc agtgaatcca gcgttggctg aatcggcgaa cctttggcgg caatcaaacg
    20281 agaatctgcg gcgtacaact tgtcggagaa agcaatctcc tgctgacgtt tatcggtaat
    20341 ggagagcgac gaaataatgg cgtcgatttt tttcgctttc agtgagggga tcagcgcgtc
    20401 aaagtcactg gcaacccagg tacatttcac ctgcatccgt ttgcacatct cgttaccgag
    20461 atcgatatca aagccaacaa agtcaccttt ggcatctttc gatgagaacg gcgcataggt
    20521 ggtatcggtt ccgatacgta ccgtctccgg tagcgccgca tagctggaag ccactgcgga
    20581 gagaccgact aacaaagaca gagcgagaat cgacttcttc atacataacc ctcaagtgaa
    20641 aatggctttt ttatgttgtg tattgtgttg tgtgtttgca ggctctttca tgcaggtctt
    20701 atgccatctt tacgacaaca gtaacattca acgttaaata tgttaataag acgttgcatt
    20761 attgtcctaa agttgaagat agcaggtatg gcggttgaat cgcagcgttt cgattcatcg
    20821 aaaggaacaa aatgtcgagg atttgatcgc ggttgcaaaa ttgccctgaa acagggcaac
    20881 aatgaggtta tgcgccctgc cagcgggcaa agagatcttc aggaagggtt atcgcaaact
    20941 ggtcaagaac acgattaacc gtctgattta tcacatcatc aagggattgt gggcgatgat
    21001 aaaacgccgg aacgggaggc ataatcaccg caccgatttc tgccgcctga gtcattaaac
    21061 gcagatggcc taagtgcagt ggcgtttcac gcacgcagag caccaacggg cgacgctctt
    21121 tcagcaccac atctgccgca cgggtcagta agccatcagt atagctatgg acaatgccgg
    21181 acagggtttt gattgaacag ggtaaaatca ccatccccaa cgtctggaaa gaaccggaag
    21241 aaatgctggc agcaatatcg cgcgcatcgt gtgtcacatc agctaacgcc tgcacttcgc
    21301 gcagggaaaa atccgtttcg agggataagg tctggcgcgc tgcctggctc attaccagat
    21361 gcgtttcgat atctgtgaca tcgcgcagaa cctgtaataa acgcacgcca taaatcgcgc
    21421 cgctggcacc gctgatgcct acaatgagtc gtttcataaa tagttgccct gtcagacttg
    21481 caggcagact ttgcaggatt tcgacgggag ttgcaagtca gggtgccaga ccggcaccct
    21541 gagcgaaggc atcatccttc gttatgcatt tcgagatttt ccacttcgtt ctgacgttgc
    21601 actgctttgg cgtcgtcatt acgtaaagta tcgaggaaat cgaggtagcc ctgatcaacg
    21661 tctttggtga cgtagacgcc gttgaatacc gagcattcaa actgctggat atccgggttt
    21721 tcagcgcgaa cggcttcgat cagatcgttc agatcctgga aaatcaaccc gtcagcaccg
    21781 atgatctggc gaatttcatc tacttcgcga ccgtgagcga tcagttccgt ggcgctcggc
    21841 atatcgatac cgtaaacgtt cgggaagcga atttccggtg ccgcagaagc gaggtacact
    21901 ttcttcgctc cggcttcgcg tgccatctcg ataatctgct cagaagtggt gccacggaca
    21961 atggagtcgt cgaccagcaa gacgttttta tcgcggaact cggcgcggtt ggcgttcagt
    22021 ttacggcgca cggacttacg acgcagctgc tggcccggca tgatgaaggt gcggccaaca
    22081 tagcggtttt taacgaagcc ctggcggtac ggtttgccca gaatacgagc aatttccagc
    22141 gcgatatcac acgaggtttc cggaataggg atcaccacgt cgatatccag atcttcccat
    22201 tcgcgggcaa ttttctcgcc cagtttggtg cccatattca cgcgcgcgct gtaaacggaa
    22261 attttgtcga tgaacgagtc cgggcgggca aagtatacat actcaaacag gcacggattg
    22321 ctgaccggat tgtcagcaca ttgacgggta aacaactgcc cttcttcagt gatgtaaatc
    22381 gcttcgcccg gcgcgacgtc acgcaggaaa tcaaagccca gcgtatcgag cgcaacgctt
    22441 tcggaagcga ccatatattc tgtacggttc tcgtcaatat cacgttttcc cagtaccaac
    22501 ggacgaatac cgtttggatc gcggaaagca accataccgt ggccgatgat catcgccaca
    22561 caggcatacg cgccgcggat taagcggttt gtagcagcga tggcggcgaa aatgttgtcg
    22621 gcttccagcg gatagtggcg gaagttgtcc agttcgctgg caaagatatt aagcagaatt
    22681 tccgagtcgg aagtggtgtt gatgtggcgg cgtttttctt caaacagttt tttacgcaac
    22741 tcgtgagcgt tggtcagatt gccgttgtgg gcaagcgtaa tgccatacgg ggagttaacg
    22801 taaaacggct gcgcttcaga ggcgctggag ctgccagccg tagggtaacg cacatgacca
    22861 atgcccatat tgccctgcaa acgctgcata tggcgagctt caaatacatc gctcaccagc
    22921 ccgttcgctt tacgcaaacg gaagcagtta ttggcatcta tggtgatgat gccggcggca
    22981 tcctgaccgc gatgctgaag caccgttaag gcatcataaa tcgactggtt aaccggcata
    23041 acaccggcga taccgacaat accgcacata cgtctttttc ctcgttaagc cacatctcag
    23101 agcacttacg ctctgggcaa gaaacttgac gagctttgca gataatcaaa aaaccatctg
    23161 atgataaaac tgaactgcgg gatcagctgt gatttgctcc agtcttcgct tttcgacacc
    23221 ccggtaaagg agtcgagaaa gaagagaatg gcggcaacaa tcaacacacc gcgcaacgca
    23281 ccgaaacaga cgcccagcac ccgatcggtg cctgacaacc ccgttttctc taccaactgg
    23341 cctatcacga agttcacgat agcaccaacg atcagggtag cgataaacag taccgcgatg
    23401 gcaatcccat ttcgaaccag ttcgtcttca aagcccgtaa accagactga caggtaagtg
    23461 tagtaatgac tggcaacaaa gaaagcacaa ccccatgtca ccagcgataa cgcttcacga
    23521 acaaagccgc ggatcaggct aaccagagag gaaaaagcaa tcaccgcgat tatggcgtaa
    23581 tcaatccaga ccatatgtgt cccacgattt tacgccctgt catcctgttc ggggcgcatt
    23641 ctaacagaaa aagaaaacgt ttgcgtaggg atttccttcc cgcgcatcaa taaaaatggc
    23701 gctgaaaaaa cattcaacgc catcgacttt ttatgccttt gcggcatcgg gcaatgcgtg
    23761 tcggatgcgg cgtgaacacc ttatccgacc tacggttcta cccctgtgta ggcctgataa
    23821 gacgcgccag cgtcgcatca ggcaagaccg tattaattcg gcgtatagcc cattaccacg
    23881 ccacttaagc cagaaagttg cttcaactca cccagcgatc ctttcagctt atctttcgag
    23941 gcatcaggcc caaccagaat acgggtaatt ttaccctgca ctggcgtgga tggcgacgta
    24001 taaacccgat aaccggcacc gcgcagctta ccgacaatct cattcacttt atcggcattt
    24061 ttcagcgcgc ccagttgcac aacataggct ttacccgttg gcgctgcttt ttcttccacg
    24121 accggcttcg acgcaggtgg tgcttcaacc ttcggtttag gcgactccac cggtttcggc
    24181 ttcggtgggg cgaccggtgc aggttccggt tcaaactcgg tgttattgac tgcaatagtg
    24241 gccggatcga gcgacggtgc tgcggcatca cctgcccgca cctcttccgc tgcgccttcc
    24301 ggcggctgcg tcggcagcgc ctgggtggcg gctggcatca tatcaggctc atcgcgatcg
    24361 cccgctttcg gcaccagcgg gatagccgcg aactcatcct gataatgttt tttctgcccg
    24421 tccagcagcc ctggaagtac aatcaccccc agcgccacca gcacgatcgt gcccactaac
    24481 cgattctgaa acttacttgc caccgcttct cctcgcgtca atcacttcca tgacatgtgc
    24541 gaccgtgtgg aacgaaccac acaccagcac ggtatcttcc gctttagcgt ccgccattgc
    24601 ggcatcccat gcctgcgcca cgctatcaaa tgatttgccg ttacccaaat gctcaagcag
    24661 ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca caataccagt catcaaccac
    24721 gcttttcaac caggccagag ttccggcaat atctttatca tgtagcatac cgataaccgc
    24781 cagcacgcgc ccgtttttcg gtagcgcttt catacgcccg gtgagatatt ccgccgcatg
    24841 tggattatgc gcgacatcaa aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc
    24901 cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt tcactgacct ccagcccgct
    24961 ggcacgcagt gccgccagcg ccgttgcggc attcggttgc gggacaagcg gcaacggcag
    25021 attttccagc gtgccgtgag catcgctaaa cgcccagtca tgatcggtga cggaatagtt
    25081 ccactcaacg ccccgacgtt gtaacagtgc acttttttcc tgcgccacat cagcaatggt
    25141 agaaggcatt tccggctcac cgacaattgc cggtttttcg ctgcggaaga tgcctgcttt
    25201 ctcgcgacca atgctttcgc gatctggccc cagccagtcg gtatgatcca gcgcaatgct
    25261 ggttactacc gcaacatcgg catcgacaat attggttgcg tccagacgac cgcccagccc
    25321 tacttccaga atcaccacgt caagttgtgc ctgtttgaac agccacaacg ccgacagcgt
    25381 accgtactcg aaataggtca gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga
    25441 ggcggtgtgg gccgattccg gcaattcctg gccctgcaca cgtacgcgct cggtataacg
    25501 caccagatgc ggcgaagtgt agacgcccac tttgtaccct gccgccatca gaatcgactc
    25561 cagcgtacgg caagtggtgc ctttgccatt cgtacccgca acggtaaaca caaatggcgc
    25621 aggtttcagg acgccaagac gcgccgcgac ctggctcacg cgctcaaggc cgagatcgat
    25681 agttttactg tgcaggtttt ccagataaga aagccacgaa gccagaggcg acgcggcttg
    25741 aggagtgcgt ttgataatca tggtatccgc tgattcgtta cggtgagaat agcaaaaggg
    25801 cagagccagt ggccctgccc ttatcagtta tcaggcctca ggttcctgat ctggtaccgg
    25861 tggcaccact acgccttcac gcggcgcttc aggattcggc gctggcagat tcatcaactt
    25921 cgccagaatg ctcgccagtt tcaggcgcat ttccggacga cggacgatca tgtcgatcgc
    25981 gcctttctcg atcaggaatt cactgcgctg gaatcctggc ggcagttttt cgcgaacggt
    26041 ctgttcgata acacgcggac cggcaaagcc gattaacgct ttcggttcag cgatgttgag
    26101 atcgcccagc atggcgaaac ttgcagaaac accgcccatg gtcgggtcgg tcagcacgga
    26161 gatgtacggc aagccgcgct cctgcatttt tgccagcgcc gcagaggttt tcgccatctg
    26221 catcagcgac atcagtgctt cctgcatacg tgcgccacca gaggcggaga agcagatcag
    26281 tgggcagtta tcttccagcg cctgctcaac ggcacgcacg aaacgtgcac ctaccacaga
    26341 ccccatggaa ccgcccataa aggcgaactc gaatgccgca gcgacaactg gcattccata
    26401 cagggtgcct ttcatcacca ccagcgcatc tttttcgccg gtttctttct gcgcagatgc
    26461 cagacggtct ttatacttct tggagtcacg aaacttcagc acatctttcg gctcaagctc
    26521 gctacccagc tccacaaggc ttccttcatc taacaggcta tgcaggcgat tacgcgctgt
    26581 catacgcatg tgatggtcac acttcggaca gacctcaaga ttacgttcca gctcagcgcg
    26641 gtataaaacc tgaccgcagc tatcacactt agtccacacc ccttcaggaa tgctcgcctt
    26701 gcgggtggga gtaatgttgc ttttaattcg ttcaatccag ctcattaggg acctttctgt
    26761 ctgaacctgg ttcgatgcca gttttatctt tggggacgca taatgccatt tttgccccca
    26821 acagaccatg aatgttgcac attaaaacat aacagcccga aactttggat aaaaaagtgg
    26881 tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg cagcgcgttt gtggcgaata
    26941 aattcgaaaa cacctggcaa tattgaaaca aaaataatgc caacaatcat cagtttaagg
    27001 ttgctctgaa tgaaggggag cgttccgaag aaataacccg cgtaggtaaa aatcagtacc
    27061 catagcaacg cgccgatgac gttaaaagcg gcgaaatgac gatacgacat gtggcccatt
    27121 cccgcaacga acggcgcgaa cgttctgacg atcggcacaa aacgggcgag aataatcgtt
    27181 ttgccgccat gtttctcata aaactgatga gtcttatcaa gataactgcg acgaaagatt
    27241 ttcgaatccg gattactgaa cagtttgtca ccaaataacc gtccaatggt gtagttcacc
    27301 gcgtcaccca caatcgcggc gatcagcatc agcaccacca tcatatggac gttgagatcg
    27361 ttggtttcca gtgatgccaa cgcaccggca acaaacagca atgaatcacc cggtaaaaac
    27421 ggcgttacca ccagaccggt ttcacagaac aaaattaaga acaaaatggc ataaacccag
    27481 acgccatact ccgcgaccag ttctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg
    27541 aggaaataaa tcaggtccat attagcctat gccttgtact cgtcatcttt caggctgtaa
    27601 ctacgttggc tacagcctga aatgttccga gcattattct tgttagtccg ccagaaatag
    27661 cgggcccatt ggcggttttg gaagatcata ccggtcaggg taatccaccg cgaccagata
    27721 cagcccttcc gcttttgccg ttgctgccgc cagcgttctg tcctttgccg ccagcaactc
    27781 tgctatccag ctctccggct ggttgtgggc accgacttcc atcaggctgc cgacaatatt
    27841 cctgaccata tgatgtacaa aggcattcgc tttgatatct accaccacat aaggaccgtg
    27901 acgcgtgaca ttaatatgca taacgttgcg ccacggggtt cgggactggc actgcaccgc
    27961 acggaacgag gtgaaatcat tctcgcccag caagcattgc gcagcccgat gcatccgttc
    28021 agcgtccagc ggttcgtaaa aatgggttac ccctttactc agtaccgccg ggcgcagccg
    28081 atgattgtag atgatgtagc gataacggcg agccgtggcg ctaaatcggg catgaaaatc
    28141 atcaggtaca gctttaaccc aacgcacggc gatgtcacca ggtaaattcg catttacgcc
    28201 caacgtccac gcagcgtctt tgcgctgtgc ggtcgtttcg aaatgcacaa cctgcccggt
    28261 accgtgtaca ccggcgtcag tacgcccggc gcagaagacg gtgatgggtt cgttcgccac
    28321 ctgggagagc gccttttcca gcttctcctg cacactgcgg acttcattct gccgttgcca
    28381 gccgtaatac ctactgccgt cgtactcaat gcccagcgca attttataaa ctggcggttg
    28441 ttgctggtcg gacattagta cagatactcc tgcaccagtt tctcggcgat ttttactgcc
    28501 atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc agaactgaac ttgctccggc
    28561 ataccgtagt cattacgtac gcagccaaca gaaagatgcg gcgtaccaga agcatctcct
    28621 acctgagtcg ggaattcgtt ctcttcagag agcacaatat cttcgccctg agcaaacgca
    28681 tcacgcgctt cttctgccgc cagcggacgc agtgcttcaa agttgaccat ctgggcatga
    28741 ccgtagaata ccggtgcctg gacgacgctt gccgaaatca tcagcccttc gtcctgcagg
    28801 attttgcgta cttcgtcaac gatacgacgt tcttcacgca cgctaccttc gctatccggc
    28861 agtaacggca gcatgttgaa cgccagctga cgcccgaaga aatcttcttc gtcaatcgga
    28921 atgccattga gtaatttagc actctgcccc gctaacgcat cgaccgcttt tttgccctgg
    28981 gcggaggctg aaatcaggct ggtaacgctg atacgtgata aaccgccctg atcaattagc
    29041 ggtttcagtg ccgccagcag ctggctggtc agactgtccg gtacggcgat gacattccgg
    29101 ttgcggtaat cggtcagtac aaacgggttt acttccggca ctaccagcgg tacgtcaggt
    29161 tcgagagcaa acaatccgct gctgtcgatc accagacaac ctgagttggt cgcttcttca
    29221 acccaggcag cggtagcttc tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag
    29281 tcaaattcag cggcatcctg cacggtgatt gtcttaccac caaagcgcag ttgttcgcct
    29341 gcgctttcgt tacgtgccag tgcataaatt tccccaaccg ggaactgacg ttcagccagc
    29401 gtttcaagca gggcttcgcc cacagcgcca gttgcgccca ggacggcaat gttccagcct
    29461 tcagacatgg tggtttactc cagaaatagc aaagctccct gccaatgtta cagcagagag
    29521 catgaagaag agattaacgt gccggatgat gaacggcgtt aaaacccagt ttacacagca
    29581 atgatgccgc actggcgtca tcacaaatta catacagaga tgaccattca cggcgctcaa
    29641 gatagttttt gcgcagttta tcgaactcac ccggtatccc ggcgacttta cgcagtggcg
    29701 catcatcgcg gcgcacatca tacaccaaat gcaccagcct tttcagcgtc ggttgatcga
    29761 gcggaccatg cagcgtaatg cgaccaaact ctggcgcggg cagtaatgta tccagcgcaa
    29821 cgtgttgttc atgcccaatg aatttgctat aggcttcaaa cacttgcgta gtaccgcgtg
    29881 ctttaccttc cagggtatag cctgcaatat gcggcgtgcc gatatccact tttgtcagca
    29941 attccacatt aagttccggc tcgccttccc agacatccag caccacgctt aacttctggc
    30001 cttcattcag gcaggtcagc agcgcggtat tatcgacgac tgcgccacgg caggcgttaa
    30061 tcagaatcgc tccgggcttc aggctacgga tcagtttttc atccgccaga tgtcgcgttt
    30121 tgtacggacc atctttaaag agtggcgtat ggaaagtcag aatatccgca tgctggacta
    30181 attcatccag cgagcggaaa tcgccctcat ccccacggtc ggcgcgaggc ggatcgcaaa
    30241 gcaaggttgt aatccctaac gcttccagtc gtgcctgtaa acgccgacca acgttaccaa
    30301 cgcccacgat ccccacggta cgctcgtgca gtgaaaatcc atcgcgttca gcaagcatca
    30361 gcagtgagga gaaaacatat tccactactg caatcgcatt acagccaggt gcagcggaaa
    30421 aaccaattcc cgcctgcttt aaccatgtgt catcaacatg gtctgtcccc gcggtggcag
    30481 tgccaacaaa tttaatgggt tttcctgcca gcaaagattc attcactttc gtgaccgaac
    30541 gcaccatcag cgcatccgcg tctgccagtt gagccacggg gatgggacgc ccgggaaccg
    30601 cggtcacctc acccaaacgg ctaaataagt cgcgggcata aggcatattt tcatcaacaa
    30661 ggatcgttaa taatttttca gtacgcgaca atctggcccc ccctttatca gcaaacaccc
    30721 ttttaacccc accgtacaac atgaaaacca tttcataatc atacacatac aaacgcaacc
    30781 atttagacgg gtgatactgg aaagtatgtt tcacagtatc acccttaaag ctatttcaga
    30841 ccagacaaaa tcactctccc gtggacaccg agtggacact tacgccacct ttcagcggat
    30901 taagagccac cgcatcctgc aaataatccg gcgcaaaatg tgcatacgcc attgtttgct
    30961 ggatagtcgc gtgacccagg atcctctgta gcgcaataat gtttcccccg ttcatcacaa
    31021 aatgactggc gaacgtatgt cgcaacacat gcgccgcctg tccttttggc aaatcgggtt
    31081 taaccctttt cagcaccagg caaaattccc ggtacttcac atcaaataat ccccccgtct
    31141 cgctggtttt gatcgcctca caaaccgcct gcgagattgg caccgttctc ttacgaccgt
    31201 ttttggtctc aagaaacgtc acacgattat gaactatctg ttcaccacga agcttactgg
    31261 cttcgcccca gcgtgccccc gtacttaaac acaaaagcgc aacacgccag taatcgccct
    31321 ctagtgtatc gagcaataac gccacttctt tctgggacaa gaaagccatt tctcgcgggg
    31381 acacataaag cacagagatt cccctgaccg ggtgctctgc atcccatagt cctatttttt
    31441 tcagagcggt aaacattccg gataaccggt tcatatacct gttagccgat gacggcttca
    31501 gtccgtcagc gatcctgcga gaacgccagg caatgatttt cagcttatcc agatccaccg
    31561 cctgcatatc agcaccaagt tcattgatta tgttgcgtaa ttgttttcgg tcttcctccg
    31621 ctttacgcct gtgctggccg tgatacaccc accacaactc cagcaaatcg ttcaacgtcc
    31681 ggcgatcacg gtagccctgt atatattccc gcttctcagc attcgccatg atgtagcgct
    31741 cagtggccac cgctaccgat tttttgttaa ataccttacg cacgcgtttt cccttgcgtc
    31801 cgttcggcct gatatccagc aaataacgac catcttcgag cttcttaatc gacatggcga
    31861 agccctccaa tgaaccgttt tacaatttca ccagcttctt cccagcaata gtcagaccag
    31921 gcaaaaagca ggtccaacca gttttctggc ctcagcgggg taattttcgg gttgtttcgg
    31981 ttgagtcctg attgccctcc aaattgcagg agccatcaag agagagagcc ggggctatct
    32041 gtccggcctc cggcattgtt tcatcagttg ataaccagta ggcgtacttc ttaaatttag
    32101 ggtgttttgt aaccttaagt aaggccccct ctgttacttg cttccccctc acctcatagt
    32161 tagttactgt cccataaggc aaccccacgc aatccgcaaa ctcctggcgg gtcatacctt
    32221 cagcttcacg tatcaggcga aatttttcac ccatgcttga caaagatgcc atatcgggca
    32281 tatcctccat tgcaaacgtg ccacatcggg cacacacata agaagccaaa ataagccact
    32341 ttgggccatt ttgagccatt cgaacgaatc aaggagatta ccacaatgag agaatcagag
    32401 cttgagggct tcattcaggt agcgccatat ccacttgagg cggtgccata tcaactattc
    32461 gccaagatga ttggccgcaa agaatccacc gtcagaacca tgattgacgc cgcaaagcta
    32521 ccaacaattg actttgtgaa accgggttca gtgaaaactc gtgcatcaga aaactgggta
    32581 tatctgccag catttaacgc aggtatgcga aaagcatttt ttaatcaacc caaagaacaa
    32641 cgcgatgcat ggctgttatg gctgggtctg taacgatata tccaggaatt tttcaaaacc
    32701 aatatatcta ctctgagaga gttgtttggg cataaaaaag cacaggataa ctaagctgat
    32761 aacccactac agacacaaaa ggaaacagcc atgactacac aaaaagcaaa aaccataatt
    32821 aaacgataca acgtacgtag tggtgtggct ggcgttgtct tttccgatgt gacacagcga
    32881 cgcagcttcc gcactggcac agaacgccac gcagcccgtt tctcaaccag tgcatcacgc
    32941 agcaacacgc actacagcct gcaggaaacg cacgccacgc ctgacgggca tctggttaaa
    33001 caaaccggta aacacacctg gctgattaat gatgccggaa ttgtcataca cagaggcgca
    33061 cgcaacccga ttaccggaaa tacctgttac tccctgactc gcggagatga gcagttcggg
    33121 caggatttca ccctgcacga agcattaaga acggcagacc ggttaatcag cggtcgcagt
    33181 tttcagcacc cg
//
LOCUS       DAZKJR010000044        32334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKJR010000044 DAZKJR010000000
VERSION     DAZKJR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.044"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..164
                     /locus_tag="ACHOWS_002070"
     CDS             <1..164
                     /locus_tag="ACHOWS_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFQ0047644.1"
                     /translation="FEDNVTPLFMKWFSNSSENNTLTALRNTLLPKLISGELSVEDLP
                     DHIKQTEAA"
     gene            259..3522
                     /locus_tag="ACHOWS_002071"
     CDS             259..3522
                     /locus_tag="ACHOWS_002071"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFQ0047645.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEANIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            3737..4075
                     /gene="symE"
                     /locus_tag="ACHOWS_002072"
     CDS             3737..4075
                     /gene="symE"
                     /locus_tag="ACHOWS_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFQ0047646.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVIEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQDF
                     IGVISSKTPR"
     gene            complement(4117..4170)
                     /locus_tag="ACHOWS_002073"
     CDS             complement(4117..4170)
                     /locus_tag="ACHOWS_002073"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFQ0047647.1"
                     /translation="MSDERLRDIGLRREDVE"
     gene            complement(4159..4314)
                     /locus_tag="ACHOWS_002074"
                     /pseudo
     CDS             complement(4159..4314)
                     /locus_tag="ACHOWS_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001684533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            4457..5869
                     /gene="yjiR"
                     /locus_tag="ACHOWS_002075"
     CDS             4457..5869
                     /gene="yjiR"
                     /locus_tag="ACHOWS_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0047648.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(6111..7055)
                     /locus_tag="ACHOWS_002076"
     CDS             complement(6111..7055)
                     /locus_tag="ACHOWS_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFQ0047649.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            7522..8754
                     /gene="mdtM"
                     /locus_tag="ACHOWS_002077"
                     /pseudo
     CDS             7522..8754
                     /gene="mdtM"
                     /locus_tag="ACHOWS_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
     gene            8795..10075
                     /gene="yjiN"
                     /locus_tag="ACHOWS_002078"
     CDS             8795..10075
                     /gene="yjiN"
                     /locus_tag="ACHOWS_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFQ0047650.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            10191..11342
                     /gene="yjiM"
                     /locus_tag="ACHOWS_002079"
     CDS             10191..11342
                     /gene="yjiM"
                     /locus_tag="ACHOWS_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFQ0047651.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            11352..12119
                     /gene="yjiL"
                     /locus_tag="ACHOWS_002080"
     CDS             11352..12119
                     /gene="yjiL"
                     /locus_tag="ACHOWS_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFQ0047652.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            12116..12373
                     /gene="ytiA"
                     /locus_tag="ACHOWS_002081"
     CDS             12116..12373
                     /gene="ytiA"
                     /locus_tag="ACHOWS_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFQ0047653.1"
                     /translation="MKEHLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            12438..13298
                     /gene="yjiK"
                     /locus_tag="ACHOWS_002082"
     CDS             12438..13298
                     /gene="yjiK"
                     /locus_tag="ACHOWS_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFQ0047654.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            13366..14544
                     /gene="yjiJ"
                     /locus_tag="ACHOWS_002083"
     CDS             13366..14544
                     /gene="yjiJ"
                     /locus_tag="ACHOWS_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0047655.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(14557..15111)
                     /gene="kptA"
                     /locus_tag="ACHOWS_002084"
     CDS             complement(14557..15111)
                     /gene="kptA"
                     /locus_tag="ACHOWS_002084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFQ0047656.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDIVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIGFIEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            15361..16044
                     /gene="yjiH"
                     /locus_tag="ACHOWS_002085"
     CDS             15361..16044
                     /gene="yjiH"
                     /locus_tag="ACHOWS_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFQ0047657.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            16041..16502
                     /gene="yjiG"
                     /locus_tag="ACHOWS_002086"
     CDS             16041..16502
                     /gene="yjiG"
                     /locus_tag="ACHOWS_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFQ0047658.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            16515..17687
                     /gene="iadA"
                     /locus_tag="ACHOWS_002087"
     CDS             16515..17687
                     /gene="iadA"
                     /locus_tag="ACHOWS_002087"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0047659.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQMLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            17752..18663
                     /gene="hypT"
                     /locus_tag="ACHOWS_002088"
                     /gene_synonym="qseD"
     CDS             17752..18663
                     /gene="hypT"
                     /locus_tag="ACHOWS_002088"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFQ0047660.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPIAERFWRELRELEIEPG"
     gene            18670..19041
                     /locus_tag="ACHOWS_002089"
                     /pseudo
     CDS             18670..19041
                     /locus_tag="ACHOWS_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(19089..19472)
                     /gene="iraD"
                     /locus_tag="ACHOWS_002090"
     CDS             complement(19089..19472)
                     /gene="iraD"
                     /locus_tag="ACHOWS_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFQ0047661.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGNWHRTMPQ"
     gene            complement(19886..20659)
                     /gene="uxuR"
                     /locus_tag="ACHOWS_002091"
     CDS             complement(19886..20659)
                     /gene="uxuR"
                     /locus_tag="ACHOWS_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFQ0047662.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(20874..22334)
                     /gene="uxuB"
                     /locus_tag="ACHOWS_002092"
     CDS             complement(20874..22334)
                     /gene="uxuB"
                     /locus_tag="ACHOWS_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFQ0047663.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(22415..23599)
                     /gene="uxuA"
                     /locus_tag="ACHOWS_002093"
     CDS             complement(22415..23599)
                     /gene="uxuA"
                     /locus_tag="ACHOWS_002093"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0047664.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIVGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            23939..25282
                     /gene="gntP"
                     /locus_tag="ACHOWS_002094"
     CDS             23939..25282
                     /gene="gntP"
                     /locus_tag="ACHOWS_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFQ0047665.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(25455..26357)
                     /gene="fimH"
                     /locus_tag="ACHOWS_002095"
     CDS             complement(25455..26357)
                     /gene="fimH"
                     /locus_tag="ACHOWS_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFQ0047666.1"
                     /translation="MKRVIYLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(26377..26880)
                     /gene="fimG"
                     /locus_tag="ACHOWS_002096"
     CDS             complement(26377..26880)
                     /gene="fimG"
                     /locus_tag="ACHOWS_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFQ0047667.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTAGFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(26893..27423)
                     /gene="fimF"
                     /locus_tag="ACHOWS_002097"
     CDS             complement(26893..27423)
                     /gene="fimF"
                     /locus_tag="ACHOWS_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFQ0047668.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAVKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(27433..30069)
                     /gene="fimD"
                     /locus_tag="ACHOWS_002098"
     CDS             complement(27433..30069)
                     /gene="fimD"
                     /locus_tag="ACHOWS_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFQ0047669.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(30136..30861)
                     /gene="fimC"
                     /locus_tag="ACHOWS_002099"
     CDS             complement(30136..30861)
                     /gene="fimC"
                     /locus_tag="ACHOWS_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFQ0047670.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(30898..31437)
                     /gene="fimI"
                     /locus_tag="ACHOWS_002100"
     CDS             complement(30898..31437)
                     /gene="fimI"
                     /locus_tag="ACHOWS_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0047671.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGKISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(31502..32056)
                     /gene="fimA"
                     /locus_tag="ACHOWS_002101"
     CDS             complement(31502..32056)
                     /gene="fimA"
                     /locus_tag="ACHOWS_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFQ0047672.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTA
                     IDGTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         7732 a         8258 c         8020 g         8324 t
ORIGIN      
        1 tctttgaaga taatgtaacc ccattattta tgaaatggtt ttctaattcg tctgaaaata
       61 ataccctaac agccctccgc aataccctgc tccccaaact catctccggc gagctatccg
      121 tggaggatct cccggatcac atcaaacaaa ccgaagccgc ataacgcatt ttgcccctgt
      181 aaaatcaggg gctttctggt aaggttttct actgatacgg gaatgcttac cagaaattag
      241 ccagggttgg agcacgatat gagtctctct ttcagtgaag caaaattaga acaagcgatc
      301 attgaactgt tacaggatca gggatatcaa cacctgatcg gcgataatgt cccacgttca
      361 agtctcgatc aggtcattat cgaagacgat ctccgtcatt atttagcggc acgctaccag
      421 cctgatggca ttactgaaga agagattcag cgactgatca aacagttcac cacgcttccg
      481 gcctccgatc tttatgaaag caacaaaaca ttttgtcgct ggctggcaaa tggttttctg
      541 ttcaaacgcg acgatcggca acaaaaagat ctctatattg aattgctcga cacccggcat
      601 ctacctgccg cactgcgcca gatatttgac gccgaagatg tcccgttgca acaggctgcg
      661 gaactcccgc cttcctatat taacccgccg cttaaccgga ttaagattgt taaccagctt
      721 aaaatctccg gcaaagataa tcagactcgt attcctgacg gcattctcta tatcaacggt
      781 ctgccactgg tcgtctttga atttaaaagc gcggtgcgcg agcaggaagc caatataggc
      841 gatgcctgga agcagctctg taaacgctac cgccgggata ttccgcaact gtttatctac
      901 aacgcgctct gcattattag cgatggcgta aataaccgga tgggcaacct gtttgcaccc
      961 tatgaatatt tttactcatg gcgcaaagtc accggtaatg aaaaccgtga acaggacgga
     1021 attccatcat tgcactcaat gattcagggg ctgtttcatc cggtacgtct gctggatgtg
     1081 attaaaaact ttatctgctt cccggataaa gccaagcacg aagtaaaaat ttgctgccga
     1141 tatccgcagt actatgccgc ccgcaaactc tattacagca tcaagcaagc gcgtaaacct
     1201 ttcggtagcg gtaaaggcgg tacctacttt ggcgcaacgg gctgcggtaa aagctacacc
     1261 atgcaatttt taacccgctt gttaatgaaa agcgtggagt ttgccagccc gaccattgtt
     1321 ttgatcaccg accgcaccga tctggacgat cagctttctg cgcaaatgtg caacgccaaa
     1381 aattacattg gcgacgacac catcgttcct gttaccagcc gtgaagattt gcgtaatcaa
     1441 ctggcgggac gcaatagtgg cggtgtattc ctgacaacga tccataaatt caccgaagac
     1501 accgaactcc tttctgaacg cagcaatatc atttgcatct cggacgaagc acatcgcagc
     1561 caggttaacc tcgaccagaa agtcatcgtc gataaagaaa gcggaaaagt gcgcaaaacg
     1621 tatggctttg cgaaatacct gcacgattca ctgccaaatg ccacctatgt tggctttacc
     1681 ggcacaccga ttgacgcgac gctcgatgtc ttcggtgagg tgatcgacag ttacaccatg
     1741 accgaatccg ttcaggatga aatcactgta cgcatcgtgt acgaaggccg tgcggctaaa
     1801 gtgatcctgg actccagcaa actggaagaa gtcgaaaagt attacgaaga gtgcgcaaac
     1861 gcaggcacca atgagtggca aatcgacgaa agcaagaaag ccacggctac catgaatgcg
     1921 gttctgggtg atgaagatcg gttaaaagcc ctcgcggaag attttgctaa acattatgaa
     1981 aaacgcgtag ccgaaggttc caccgtaata ggcaaagcca tgtttgtttg tgccagccgt
     2041 gaaattgcct gggatttcta ccgccagctt aaagctattc gccctgcctg gtttgaagtg
     2101 aagcaagcac ctgatggcgt cgtcctgacg gaacaggaac aaaaagagtt accgccttct
     2161 gaaatggtga aaatggtcat gacgcgcggt aaagatgacg acgaggcgct ttatgattta
     2221 ctgggcacga aagaatatcg taaagagctg gataagcagt tcaaaaacgc taaatcgaat
     2281 ttcaaaattg ccattgtcgt tgatatgtgg ctgaccggtt ttgatgttcc tgaactggat
     2341 accatctata ttgataagcc cttacaaaaa cataacctta tccagactat ttctcgcgtt
     2401 aaccgtaagc tggaaggcaa aagcaaaggg ttagtggtgg actacatcgg cattaaaagt
     2461 cagatgaacc aggcactggc aatgtattcc cgcattgatg ccaccaactt tgaagatatt
     2521 cagcaatcgg taatagaagt taaaaaccat ctcgatttgt tggggcaagt cttttacgaa
     2581 tttgacagtc gggattattt tagtggtgag ccacaagcgc aattatcctg cctcaaccgc
     2641 gcggcggaat ttgttctgcg tacccagaaa gttgaacgtc gttttatggg actggttaaa
     2701 cgcatgaaag ccgcctacga cgtctgctgc ggcagcgaag cattatcaca gacagaacgt
     2761 gatcatattc actattatct tgctgttcgt tcaattgttt tcaaactgac gaaaggtgac
     2821 gcaccggatg tcacccagat gaatgcacgc gttcgtgaaa tgattgcaga agcgctaaaa
     2881 gctgatggcg tagaagaaat ttattttctt ggcgataaaa aagcggaatc catcgatatt
     2941 tttgacgaag attatctggc gcgaattaac aagatcaaac ttccggcaac aaagatccag
     3001 ttattacaaa aattactgga aaaagcgatc agcgacttca agaaagtgaa ccagttgcaa
     3061 gggattaact tcacccgccg cttccaggcg attatagatc gttataatga gcggcgagaa
     3121 gatgatgtac tcaacggtga agaattcgat acattcagtc aggaaatgac cgatattatc
     3181 tatgatatta aaaccgaaat gggcacctgg gccgatttag gcattgatat tgaagaaaaa
     3241 gcctttttcg acattcttgc tcatatgcgc gataaatatc agttcaccta tgacgatgaa
     3301 aaaatgctgt cactggcaaa agagatgaaa agcgtggttg acaacacatc gaaatatcct
     3361 gactggagta aacgcgatga tattaaagcg aaactgaaag ttgaacttat tctgcttcta
     3421 cacaagcata agttcccgcc agtagcgaat gatgatgttt atatgggtgt gctggcacag
     3481 gcagagaatt ttaaagaaca tcatatgagt gctttaaatt aattatgaag gccggataca
     3541 ctttgttatc cggccattct gaattaacgt taatttctct tccagatctt tccagtaacg
     3601 cttttattat tttctatttt tagctggact ttccctgtat ttactgatga tatatccagg
     3661 tatttagcgc ggtgcgaatg tgcgccaaca cacccgcacc gctcatcaca atccctattt
     3721 cacgagaata gtaatcatga ctgacacgca ttctattgca caaccgttcg aagcagaagt
     3781 ctccccggca aataaccgtc aattaaccgt gagttatgcg agtcgctacc cggattacag
     3841 ccgtattccc gccatcaccc tgaaaggtca gtggctggaa accgccggtt ttgccaccgg
     3901 cacggcggta gatgtcaaag tgatagaagg ctgtattgtc ctcaccgccc aaccacccgc
     3961 cgccgaagag agcgaactga tgcagtcact gcgccaggtg tgcaagctgt cggcgcgtaa
     4021 acaaaggcag gtgcaggatt ttattggggt gattagtagc aaaacgccac gctaagtttc
     4081 tctccccttc cccatacggg aagggaaatt gccccctcac tccacatcct ccctgcgtaa
     4141 cccgatatcc ctcaaccgtt catcactcat ctgctgtaca cccgccgggc ctgcatttgc
     4201 cgccgccaac gcctcaccgc ctgtcagagt tgcacaagtc tgataaacgg cgttttggct
     4261 ttgttttcgt gaaattccat ctgctgcctc ctcatctttc agaggcttca tcatccgtct
     4321 tctggcagtt cacaatacag atgcaaatat tagttttatt taacatacag acaggttaaa
     4381 acagcatctg catccggttt tttagcctca tctgtaccgg tttttctctc tgtatggtcc
     4441 aaataaggaa tacagcatga cgcgttatca acatctggcg actctacttg ccgagcggat
     4501 tgagcaaggg ctgtatcgtc acggggagaa attgccgtcg gtgcgcagct taagtcagga
     4561 gcacggcgtc agtattagca ccgtgcagca ggcgtaccag acgctggaga cgatgaagct
     4621 catcactccg cagccgcgtt cgggttattt tgtcgcacaa cgtaaagccc agccgccagt
     4681 accgccgatg acgcgtccgg tgcagcgccc ggtggaaatt acccagtggg atcaagtgct
     4741 ggatatgctg gaggcgcata gcgacagttc cattgttccg ttaagtaaaa gcacgccgga
     4801 tgtcgaaacg cccagcctga aaccgctgtg gcgggagcta agccgggtgg tgcagcataa
     4861 tctgcaaacc gttctcggtt atgacttgtt agccggtcag cgagtgttgc gagagcagat
     4921 tgcccgcctg atgctcgaca gcggctcagt ggtcaccgct gatgacatca ttatcaccag
     4981 cggctgccat aactcgatgt cgctggcgtt aatggcggta tgtaaaccgg gcgatattgt
     5041 cgcagtcgaa tccccctgtt attacggttc aatgcagatg ctgcgcggta tgggcgtgaa
     5101 agtgattgaa atcccaaccg atccagaaac tggcatcagc gttgaagcgc tggaactggc
     5161 gctggaacag tggccgatta aaggcatcat tctggtgcca aactgtaata atccgctggg
     5221 atttattatg ccggacgcac gcaaacgggc cgttctctct ctcgctcagc gtcatgatat
     5281 tgtgattttt gaagatgatg tctacggcga actggcgacg gagtatccgc gcccgcggac
     5341 cattcattcc tgggatatcg acgggcgagt gctgctgtgc agctcgttca gtaaaagtat
     5401 tgcaccaggc ctgcgcgtgg gttgggtcgc accggggcgt tatcacgata aactgctgca
     5461 tatgaaatat gccatcagca gctttaatgt accgtccacg caaatggcgg cggcaacgtt
     5521 tgttctggaa ggtcactatc atcgccatat ccggcggatg cggcagatct atcagcgcaa
     5581 tttggcgctt tatacctgct ggatacggga atattttccc tgcgaaatct gtattacgcg
     5641 cccgaaaggc ggatttttac tgtggataga attgcctgaa caggtcgata tggtttgcgt
     5701 tgcgcggcag ctgtgccgca tgaaaatcca ggtggcggca ggctcgattt tctcagcttc
     5761 cggcaaatac cgtaattgtc tgcgcatcaa ctgcgctttg ccgctcagcg aaacctatcg
     5821 cgaagcactg aagcaaattg gcgaggccgt gtatcaggca atggaatgaa ttcgaattag
     5881 tggcgaggcc cctgatttcc tctcacgaat cgcctgtgac tcgttttatc tttgcagggg
     5941 catcgccacg ctccagcatg gcttaggcaa agtgtgcttt gttcataccg gatgcggcgt
     6001 gaacgcctta tccggcttac aaatcgcgca aattcaatag attgcagagg taatgtaggt
     6061 ctgataagcg tagcacatca ggcagctttg cgtttgtcat cagtctctga ttagtactcg
     6121 atccatggat cgcgcttgct ttcaggcaag ggttgctcta atgcctgcat ttgctctgcc
     6181 gaaagtccag tatatcgttg tatccattca ggatccgcac cgttctgcaa aagcaatcta
     6241 aggaggcgtt gttccccttc ttctttcccc ttttccatcc ctaacagcca tccatcatta
     6301 tgaattcgct ctgcaatcgt cattattcgc tccctttgtt gtggcattcg acgggtaagt
     6361 tcactgataa actcattaaa acgcgcttca tcgccagtca gtaaaatgta atttaacagc
     6421 gcagttatct ggctgtcatt agcacactct gtaaccagta atattaccag ttgatcgata
     6481 agccccatca gatcgcgctg gcgaatatgc ttttggatca actccaacag ggcgactctg
     6541 cgatgctgca caatctcgtc gtctggcacg acagtgacat ccaccagcgg gaacgctgcg
     6601 gtataaagct tccgtgcggt ggtcgggtca gcaaattcgt ccagccagca cagggaccag
     6661 ggataaggac tacggctacc gtgataaaac agcatcggga tgaccagcgg tagcggtcgg
     6721 cgtttatcat gctcgatatg gcgctgcatc accgccatgg aatatcgcat caggcgaaag
     6781 gccatatgga tatcctcgcg gctctgatgt tcaatcacta cgtaaatata accatcacct
     6841 tcacgggtct ttaccgacca cagaatatcg gagtgtagcg cccgcaattt ttcatcgacg
     6901 aagctggcgg attccagttt taagctgtcg agatcgcaca gttcacgtaa atctttgggt
     6961 aagtggatct ccataaaatc ccgcgcggtg tcagggtgcg tgagaaagga tttaaataat
     7021 gcgtcatgcg gcgtactggt cgtgaagttt gtcatggcga tccgtcacct gttgataaga
     7081 tggcccgact ttagcgacta acaaaaccat tctcacccgg taatttccca ttccccgagg
     7141 ggctttccag gctattttta ttcttctgca aacgatacat ttttccggaa acaggctcgc
     7201 attttacatt tcctgtaccc ggcagatatt cccctttcca ccgcactctt ttgattaacc
     7261 gcaaatttta attacactta aggtgtatat tttctatgca acccatcaat tcaagaggtg
     7321 taatgtgctg atgactattt gtaatcgtta tacattctga cctggagtca gaaggtattt
     7381 ctctgtctgt gtattcacag gcagtgtggt tgattacatg aaattcagta catttatcct
     7441 tctcgcctgc ctttcgtcat taccgacggt attgaatttc gttttcccca ttggggttct
     7501 ccggacaagg agttgtttgt tatgccacgt ttttttgccc gccatgccgc cacgctgttt
     7561 ttcccgatgg cgttgatttt gtatgacttt gctgcgtatc tgtcgacgga cctgatccag
     7621 ccagggatca ttaatgtggt ccgtgatttt aatgccgatg tcagtctggc ccctgctgcc
     7681 gtcagtctct atcttgctgg cggtatggcg ttacagtggc tgctggggcc gctttccgac
     7741 agaattggcc gcaggccggt gctgattacc ggggcgttaa tttttaccct tgcctgcgcc
     7801 gcgacaatgt tcaccacgtc aatgacacaa tttcttatcg cgcgtgcaat tcagggcacc
     7861 agtatctgtt ttattgccac cgttggttat gtcacggtgc aggaggcgtt tggacagaca
     7921 aaagggatca agttgatggc gattatcacc tccatcgtac tgattgcgcc gattatcggc
     7981 ccgctttccg gcgcagctct gatgcacttt gtgcactgga aagtcctttt tgccatcatt
     8041 gcggttatgg gttttatctc atttgttggc ttactgctgg cgatgccaga gacggtgaag
     8101 cgcggcgcgg ttccatttag cgccaaaagc gtcttgcgcg attttcgtaa tgtcttttgc
     8161 aatcggctgt tcctctttgg cgcagcaacc atctctttaa gctatatccc gatgatgagt
     8221 tgggtggctg tctcgccggt gatcctgatt gacgcaggcg gcttaacgac ctcgcagttc
     8281 gcctgaacac aggttccggt gttcggcgcg gtgattgtcg cgaatgccat cgtggcgcgt
     8341 ttcgttaaag atccgaccga accgcgcttt atctggcgcg ccgtacccct tcaactggtt
     8401 ggcctcgcgc tgttgattat cggcaatctg ctgtcaccgc acgtctggct gtggtcggtg
     8461 ctgggcacca gtctgtatgc tttcgggatt ggtttgattt tcccgacctt attccgcttt
     8521 acgctgtttt ccaataactt accgaaaggg accgtctccg catcgctgaa tatggtgatc
     8581 ctgatggtga tgtctgtctc ggtcgaaatc ggccggtggc tgtggtttaa cggcggtcgc
     8641 ttgccgtttc atctgttagc cgtcgtggcg ggcgttatcg tcgttttcac cctggcggga
     8701 ttgctcaatc gcgtgcgcca acatcaggct gccgagctgg cggaggagcg gtgatttttg
     8761 cgcgatcccg ccgtcaggct ctattcttaa cattatgaat aaactcattg aactcagacg
     8821 cgccaaaatg ttggcgctct ctttactgct tatcgccgct gctacctttg tcgttacgct
     8881 gtttttgccg cccaattttt gggtgagcgg cgtgaaggcg attgctgaag cggcgatggt
     8941 cggcgcgctg gcggactggt ttgcggtggt ggcgctgttt cgccgcgtgc cgattccgat
     9001 catttctcgt catacggcga ttatcccgcg taataaagac cggattggcg aaaatctcgg
     9061 tcagttcgtg caggaaaaat ttctcgatac ccagtcgctg gtggcattga ttcgacgtca
     9121 cgaaccggcg ttgttgattg gcaactggtt tagtcagccg gagaacgccc gccgcgttgg
     9181 tcagcatctg ttgcagatca tgagcggttt tcttgaactg accgatgatg cgcgtattca
     9241 gcgcctgctt aagcgcgcgg tccaccgggc gattgataag gttgatcttt ctggcaccag
     9301 tgcgttgatg ctggagagta tgaccaaaaa cgatcgtcat caggtgctgc tcgatacgct
     9361 gatcgcacag ttgatcgccc ttctccagcg cgataaatcg cgcaagttta tcgcccagca
     9421 aattgttcgc tggctggaga gcgagcatcc actgaaagcc aaaattttgc ccactgaatg
     9481 gctgggcgaa catagcgcgg agttggtttc tgacgcggtg aattctttgc ttgatgatat
     9541 cagccgcgat cgtgcgcatc agatccgcca tgcgtttgat cgcgccacct ttgccctgat
     9601 cgacaagctg aaaaacgatc ctgaaatggc agcgcgagcc gatgccgtaa aaagctatct
     9661 gaaagaagac gaagctttta accgctatct cagtgaattg tggggggatt tacgggaatg
     9721 gctgaaagtg gatatcaaca gcgaagattc tcgtgtgaaa gaacgtatcg cacgagcggg
     9781 tcaatggttt ggcgaaacgt taattgccga tgatgccttg cgggcgtcgt taaatggtca
     9841 tctcgaacaa gccgcgcacc gcgtcgcgcc tgagttttcc gcattcctga cgcgccacat
     9901 cagcgatacg gtaaaaagct gggatgcgca ggatatgtcg cggcaaatcg agttaaatat
     9961 cggcaaagat ctgcagttta tccgtgtcaa cggtacgctg gttggcggtt gtattggact
    10021 tattttgtat ttgctgtcgc agctcccggc cttgttcccc ctcggcaatt tatagaaatc
    10081 catgaaaaac taagtggcta accgcgagag agatcaaata aaccacacta aaagaatggt
    10141 aataatgtgg cctcttttca ttatctcccg tggtacgggg aaggaaaatc atgtcacttg
    10201 tcaccgatct acccgccatt ttcgatcagt tctctgaagc tcgccagaaa ggctttctca
    10261 ccgtcatgga tctcaaggag cgcggcattc cgctggttgg cacctattgc acctttatgc
    10321 cgcaagagat cccgatggca gccggtgcgg ttgtggtttc gctatgttcc acctctgatg
    10381 aaaccattga agaagccgag aaagatctgc cgcgcaacct ctgcccgctg attaaaagca
    10441 gctacggctt cggcaaaacc gataaatgcc cctacttcta cttttctgat ctggtggtcg
    10501 gtgaaaccac ctgcgacggc aaaaagaaaa tgtatgaata catggcggag tttaagcccg
    10561 ttcatgtgat gcaactgcca aacagcgtta aggacgatgc ctcgcgtgca ttatggaaag
    10621 ccgagatgct gcgcttgcaa aaaacggtag aagaacgttt tgggcacgag attagcgaag
    10681 atgctctgcg cgatgccatt gcgctgaaaa accgcgaacg tcgcgcactg gccaactttt
    10741 atcatttggg gcagttaaat cctccggcgc ttagcggcag cgacattctg aaagtagtct
    10801 acggcgcaac cttccggttc gataaagagg cgttgatcaa tgaactggat gcaatgaccg
    10861 cccgcgttcg tcagcagtgg gaagaaggcc agcgactgga cccgcgccca cgtattttaa
    10921 tcaccggctg cccaattggc ggcgcagcag agaaagtggt gcgcgcgatt gaagagaatg
    10981 gcggctgggt tgtcggttat gaaaactgca ccggggcgaa agccaccgag caatgcgtgg
    11041 cggaaacggg tgatgtgtac gacgcgctgg cggataaata tctggcgatt ggctgctcct
    11101 gtgtttcgcc gaacgatcag cgcctgcaaa tgctcagcca gatggttgaa gaatatcagg
    11161 tcgatggcgt agttgatgtg attttgcagg cgtgccatac ctacgcggtg gaatcgctgg
    11221 cgattaaacg tcatgtgcgc cagcagcaca acattcctta tatcgctatt gaaacagact
    11281 actccacctc ggatgtcggg cagctcagca cccgtgtcgc ggcctttatt gagatgctgt
    11341 aaggagtggc agtgacatat tcgattggca ttgattccgg ctcaaccgcc accaaaggga
    11401 tcttactggc tgacggcgtg attacgcgcc gtttcctcgt tccaacatcc tttcgcccgg
    11461 caacagcaat tactgaagcc tgggaaactc tgcgcgaagg gttagagaca acgccgtttc
    11521 tgacgctcac cggctacgga cggcaactgg tggattttgc cgataaacag gtgacggaaa
    11581 tctcctgtca cgggctgggc gcacggtttc ttgcgccagc aacgcgcgca gtaatcgaca
    11641 tcggtggtca ggacagcaaa gtgattcagc ttgatgatga cggtaacctg tgcgatttcc
    11701 tgatgaatga caaatgcgcg gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc
    11761 ttggcaccag cgtcgaacag ctcgacagca ttaccgaaaa tgtcacgccg cacgccatca
    11821 cgagtatgtg cacagtgttt gctgaatcag aagtgatcag cctgcgctct gcgggcgtag
    11881 cgccagaagc gattctcgcc ggagtgatta acgcgatggc gcggcggagt gccaatttca
    11941 ttgctcgtct ctcctgtgaa gcgccgattc tgtttactgg tggcgttagc cactgtcaaa
    12001 ggtttaccca catgctggaa agccatctgg gaatgccggt acaaactcac cctgatgcgc
    12061 aattcgctgg tgcaattggc gcggcggtga tcggtcagcg gcagagaaaa cgcgcatgaa
    12121 agagcatctg tttttatttc actccacggt cggcgtcata caaacccgca aggcgttaca
    12181 ggcagcgggc atgacttttc gcgtcagcga tattccccgt gatttacgcg gcggctgcgg
    12241 gttatgcatt tggctgacct gtccgcctgg cgaggaaatt caatgggtga tccctgggca
    12301 tacggaatca gtttattgtc agcaggatgg tggctggcgc tgcattgcac attacgggat
    12361 ttctccgcgt taaggattca ttaagatcgc tacgtatact tctcgatatc ttattgattt
    12421 gccttaaagg gattaccgtg acaaaaagta tttcattaag taaacgcatc ttcgttattg
    12481 tgattctttt tgtcattgtg gcagtgtgta ctttcttcgt gcaatcttgc gcccgtaaaa
    12541 gcaatcatgc agcatcattt cagaattacc acgccaccat tgacggcaaa gaaattgcag
    12601 gcataacgaa taatatctct tcgctgacct ggtcggcaca aagcaatact ctttttagca
    12661 ctatcaacaa acctgccgcc attgttgaaa tgaccaccaa tggtgatttg atccgcacaa
    12721 ttcctctgga ttttgttaag gatctcgaga cgatcgaata cattggcgat aatcagtttg
    12781 tcattagcga tgagcgcgat tacgccattt atgtgatttc actgacccca aactcggaag
    12841 taaaaatcct caaaaaaata aaaatcccgc tgcaggaatc cccaaccaat tgcggttttg
    12901 aaggattggc ctattctcgc caggatcaca ctttctggtt ttttaaagag aaaaacccga
    12961 ttgaggtcta caaagttaac gggctattaa gtagcaatga attgcacatc agcaaagaca
    13021 aggcactaca acggcaattt actttagatg atgtgtccgg cgcagaattt aaccaacaaa
    13081 aaaatacgct tctggtgctg tctcatgaat ctcgcgcact ccaggaagta acgctggttg
    13141 gcgaggttat cggtgaaatg tcactaacca aaggcagtcg aggtttgtcg cataacatca
    13201 aacaggccga aggggttgcg atggatgcct ctggtaatat ttatatcgtt agcgaaccaa
    13261 atcgttttta tcgctttacc cctcaatcat cgcattaaca ggctggcatg cttcaccttt
    13321 ttgttattaa cttgttaaca acaaaaatga caaacaaggt taaacatgcc ttcgtccacg
    13381 catcccgtag aacgcttttc tttcagcacc gcgcttttcg ggatgctggt tctgacctta
    13441 ggtatgggtt taggccgctt cctttatacg cctatgttgc ccgtcatgat ggcggaagga
    13501 tcgttttcat ttagccagct ctcgtggatt gccagcggca actatgcggg gtatctggct
    13561 ggcagtctgc tattttcttt tggcgcattt caccagccat cgcgcctgcg cccatttctg
    13621 ttggcttctg ccctggcgag cgggttgttg atcctcgcaa tggcatggtt gccgccattt
    13681 attctggtgt tattgattcg cgtcctggcg ggtgtcgcca gcgccggaat gctgattttt
    13741 ggttcgacgc tgattatgca gcacacgcgc catccttttg tgctggcagc tttgttttct
    13801 ggcgttggca ttggcatcgc actgggcaat gaatatgttc tggcaggcct gcattttgac
    13861 ctctcctcgc aaacgttatg gcaaggcgcc ggggcgcttt ctggcatgat actgattgca
    13921 cttacgcttt taatgccctc gaaaaaacac gccatcgtac caatgccatt ggcaaaaacg
    13981 gagcaacaga taatgagctg gtggttactg gctattctgt acggcctggc gggatttggt
    14041 tatatcatcg tcgcgactta cctgccgctc atggcgaaag atgcaggctc accattgtta
    14101 accgcccatc tctggacgtt agtcggctta tcaattgtgc ctggttgctt tggctggcta
    14161 tgggccgcaa aacgttgggg ggctttgccc tgcctgacgg cgaatttgct ggtgcaggct
    14221 atctgtgtgc tgcttactct cgccagcgac tcgcctctct tgcttatcat cagcagtctt
    14281 ggttttggcg gtacctttat gggcacgaca tctctggtga tgactatcgc ccgccaactg
    14341 agcgtaccgg gaaatctcaa tcttttaggc tttgtgacgc tcatttacgg cattgggcaa
    14401 atcctcggcc cggcgctgac cagtatgctc ggcaatggaa catcggcgct tgccagcgcc
    14461 actctctgcg gcgcggcggc gttgtttatt gctgcgctta tcagcacagt gcaattattt
    14521 aagctacaag tggtcacttc gtgagagaag tttcccttac cactcaagaa actcaaccgc
    14581 aactgttgat gtcagccaga ccccgttttc cgcttgccaa aaagggatgc ctcgtttcgc
    14641 catttcctgt gctttgacgg ttaaaataac tggcgatccg tgtcttgctc ccactttgcg
    14701 tgccgttgct tcatcagcag agagatgcac gtaatggcgc tcccctgcaa tcagcccttg
    14761 tttttttatc tcgtcaagaa aacggcttgc cgtaccatgg tagagaaatt gtggcggtgt
    14821 tttttcgatg aagccaatgg cgacctgcga agtcgaatgc ccctgaacgg cgcgaataca
    14881 cgtaccatca ctggaataac taaagcgctt tttatcgctg gtagcgacaa cgatatccag
    14941 taatgcgcgg gttaaccgct tgcccgcttt ttgggcacag agtatgagct tatcgatatc
    15001 cgcccatcct tcacggtcca gcacgatgcc aatcgcctct ggtttatgtc ggaggacaaa
    15061 acttaaaaac ttgctcgtct ctgccagctc tttgtcgttg tattttgcca tctcgttctc
    15121 gtccctggat tttctgtttt tatttactga tgtcattgtc actgggttta tgaattttct
    15181 gcaactcaat cctgcgcaga acctttgcca cacagcgtca tttttccgct atgcaaaaaa
    15241 cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata aggtttttca
    15301 gctccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt
    15361 atgacacaac aaggggatgc tgtagccggg gagctggcga cagaaaaagt gggcattaaa
    15421 gggtatctgg ctttttttct caccataatc tttttttccg gtgttttttc cgggacagac
    15481 agttggtggc gcgtttttga tttttccgtg ctgaacggat cgtttgggca actgcctggt
    15541 gcgaatggcg ccaccacctc gtttcgcggc gcaggtggca caggagctaa agatggcttt
    15601 ctcttcgcgc tggaactggc accgtcagtc attctctctc tggggattat ttcaattacc
    15661 gatggtcttg gcggattacg cgccgcgcag cagttaatga cgcccgtgct aaaaccgttg
    15721 ttgggcattc caggcatctg ttcactggcg ctgattgcta acctgcaaaa caccgatgcc
    15781 gccgcaggta tgaccaaaga actggcgcag gaaggggaaa ttaccgaacg tgataaagtg
    15841 atattcgccg cctatcaaac cagcggtagc gccatcatca ccaactactt ctcttccggc
    15901 gtcgcggtgt ttgcctttct gggcacgtcc gtaatcgtac ctttagccgt catcctggtg
    15961 tttaaatttg tcggagccaa cattttgcgc gtctggctta acttcgaaga acgtcgcaac
    16021 ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa aaacgtcatg gatatgttta
    16081 ttgatggcgc gcgccgtggc tttactatcg ctaccaccaa cctgctgcca aacgtggtga
    16141 tggcctttgt catcatccag gcgctgaaaa ttactggcct gctcgactgg gtgggacata
    16201 tctgtgaacc cgtaatggcg ctgtggggat tgcccggtga agcggcaacc gtccttttag
    16261 ccgcattgat gagcatgggc ggcgcagtcg gtgttgccgc cagtctggca accgctggcg
    16321 cattaaccgg ccacgatgtc accgttttac tgcccgcgat gtatttaatg ggcaacccgg
    16381 tgcaaaatgt cggtcgctgc ctgggaacag ccgaagtgaa tgccaaatat tacccgcata
    16441 tcattacggt ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag cttattgttt
    16501 aacaaggagt taccatgatt gattattccg cagccggttt taccctgctg cagggagcgc
    16561 atttgtatgc gccggaagat cggggaattt gcgatgtcct catcgctaac ggcaaaatta
    16621 ttgccgttgc cagcaacatc ccttctgaca ttgtaccaga ctgcacggtt gtcgatctca
    16681 gcgggcagat cctctgccca ggttttattg atcaacacgt ccatttgatt ggtggtggcg
    16741 gcgaagcagg tcccacgaca cgcacgccgg aagtggcgtt aagtcgtctg acggaagcgg
    16801 gcgtcacgtc agtggttggt ctgctgggta ccgactctat ctctcgccac ccggaatccc
    16861 tgctcgccaa gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg
    16921 cttatcatgt cccttcccgc accattacag gttccgtgga aaaagacgtg gcgattatcg
    16981 atcgcgtgat tggtgtgaaa tgcgccatct ctgatcaccg ttctgccgca cctgacgttt
    17041 atcacctggc taatatggcg gcggaatccc gcgttggcgg tttgcttggc ggtaaacctg
    17101 gcgtcaccgt gttccacatg ggcgacagca aaaaggcgtt acagcctgtc tatgacctgc
    17161 tggaaaactg cgatgtgccg atcagcaagc tgctgccgac ccacgttaac cgcaacgttc
    17221 cgttgtttga gcaggcgctg gagttcgcgc gcaaaggcgg caccatcgac atcaccagta
    17281 gcattgacga accggtcgcc cctgccgaag gtattgcccg cgccgttcag gcgggtattc
    17341 cgctggcacg cgtcaccctc agctccgacg gcaacggtag ccagccgttc tttgatgacg
    17401 aaggaaattt aacccatatc ggtgttgcag gttttgaaac attgctggaa accgtgcaaa
    17461 tgttggtcaa agactatagc ttcagcctta gcgatgccct gcgcccgctc accagtagcg
    17521 tagccggttt ccttaacctg accgggaaag gcgaaattct gccaggcaat gacgcagact
    17581 tactggtcat gacgcctgaa ttgcgcattg agcaggtata cgctcgcggc aaactgatgg
    17641 tcaaagacgg caaagcctgc gtgaaaggaa cgtttgaaac ggcataaccg gttgtcagcc
    17701 gcccgaatgg acgtagaatg cccgcaaggg cggctgacag agtataacgt aatggatgac
    17761 tgtggtgcgg ttttgcataa cattgaaacc aaatggcttt atgattttct gaccctggaa
    17821 aaatgtcgca atttttccca ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc
    17881 cgccgcatcc gtgcactgga acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg
    17941 ccgctgcaac tctcggaaca agggaaaatc ttccattcgc agatccgcca tctgttgcaa
    18001 cagttagaaa gcaacctggc agagctgcgt ggcggcagcg attacgcgca acgtaaaatc
    18061 aagatagccg ctgcacactc tctttccctc ggtctgttac cgtccattat cagccagatg
    18121 ccgccgctct ttacctgggc aattgaagcc attgatgtcg atgaagcggt cgataaactg
    18181 cgcgaagggc aaagtgactg tattttttcc tttcacgacg aagatttgct ggaagcgccg
    18241 tttgaccaca ttcgcttatt tgaatctcaa ttattccccg tctgcgccag tgacgaacac
    18301 ggagaagcac tgtttaacct cgcgcagcca cactttccgt tactgaatta cagccgcaac
    18361 tcctacatgg ggcgattgat taatcgcacc ctgacgcgcc acagtgagtt gagtttcagc
    18421 accttttttg tctcttcgat gagcgagcta ttaaagcagg ttgccctcga cggctgtggg
    18481 attgcctggc tgccggaata cgctatccaa caagaaattc gcagcgggca gctcgttgta
    18541 ctgaatcggg aagaactggt gatcccgatt caggcctacg cataccggat gaatacccgc
    18601 atgaatccaa ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgaacctggt
    18661 tgaaagtttt ctgcagaaaa tcttccggcg ctgggaatta tgattgaggt tcctgccgca
    18721 gtcatgattg cagacaaatt agccagtgaa gtcgatttct tttccatcgg caccaacgat
    18781 atgacgcaat atattatggc ggcagacaga ggaaatagca ccgtcgcgaa actggtcgat
    18841 tattgtaatg acgcggtgat taacgctatt gctatggtgt gtcaggccgg acgtaataat
    18901 gaaattccgg tcagtatgtg cggcgaaatg gctggagata tccagcaaac agcacgactg
    18961 ctcaccatgg ggattgataa gctcagtgcc agtccatccc ggcttccggc cttgaaagca
    19021 gcaatcagag cctcccacta attgtgacct aacgcgatac gtcttgcgcg tatcgcgttt
    19081 cccgtcattt actgcggcat cgtgcgatgc cagttcccct tctgccagac caggtcaaac
    19141 gttagcggct catcatcgtg gtacagcatc atctccagac taatggccaa ctcgtggcaa
    19201 taggcttctt tcagattgac ctgcaatgcc gcaatacgtg gctcatgaaa taaaagcgcg
    19261 gaacgtaact ggcggcaata ccaactctgg agaagcttgt cgctctgatt atgcccttgc
    19321 cactcaggca tgcctgaaac aagtaatatt ggtagcggtg aatggggaga actaaaaagt
    19381 tgaaagagat cccggcatac ggaatcacgc agcgataacg tattgtgtcc tgtactttca
    19441 ggtaataccg tctgaactga ttggcgcatc attttgcgca ctcctgacgt ttagtggcat
    19501 ctgtatcctg ccctggctgc cggacaccac tgggttgatg ctcatattcc ataatcattc
    19561 ctttatgctc attgcgtccc tggcactttc ataagggatt tataagatcc gccaaagata
    19621 aaacactgta cagaaaagaa tatccactga ctttttattt aaaaaggtat catttgacaa
    19681 aagtcattaa acataacgga aatacgttaa catattctga ttattccgaa taatttctca
    19741 gcgttaaact gatatataat tattccaaaa caatatatcg tacgtaatga ttaatttacg
    19801 cataatatat tctgcagaac ataattaact attcagggaa catacatagt gcaaagcggt
    19861 tcaactttgc accagaaatg agggatcagg cgtcgacttt atccagcggc catgaatcaa
    19921 acagatagcc gtcaaaataa atatcgtcaa cgttcgagaa ttccagcaga cgttgtttaa
    19981 cgttttccag atgctgccac attgccagct tcgccgcacg ggcatctttt ttgattaacg
    20041 cggcgaggat ctgtttgtga tcgcccaacc actctttgcg atacaggctg tcatcgagat
    20101 ggctgtgcaa ttggatccac attggattgt tttcccgcca ctgccaggac tgacggaata
    20161 actccaccag catgctgtta tgcgttgctt cggcaatggc gagatggaac tgcatatcac
    20221 cgctttcgct gctacccggc gcgctggaag ccagttcacg ctcttccagt tgcaatgcct
    20281 gacgcatttt gacgatatct tcgcgggtag cctgcaaagc ggcaaattcc gcgatgttac
    20341 tttccagtaa ctgacgcgcc tgtaacagct caaaaggacc ggcatcatta cagacgttgg
    20401 catccggact gtctgtgttg tggctgcctg agctatcaag aacataaata ccggcacccc
    20461 ggcgcacttc caccagccct ttgatctcca gcatgatcag cgcttcacgt accaccgtcc
    20521 gcgtgacatc aagcatttct gcaatttcac gctccggtgg cagccgttcg ccaggattgt
    20581 acggcgtctt tatgatcaga tcgcggatca tcgccccgac ttcctggtaa ggtctttgcg
    20641 cagaggtggc agatttcatg gtgttgtgtc cgttaggtgc ggttaatcaa attttttccg
    20701 gatgcggcgt gaacgcctta tccgacctac aacgggcaat tttacggcac ccgttaagct
    20761 caaactatac cattagcact ggatgttggc acgtacaggt gggcaaaaaa acgaaaactg
    20821 gtcaaccaaa tattagtttg attgaccagt aagtctgtag ggtaatcagt tagttacagc
    20881 gcagccacac actcgcgcgc accgcgctca cacagctgct gatatgccgc cgtcaccgcg
    20941 ccaacaaagt cggcgttctg cggcagatca tcggcaaaga taccgctcag gcccagcagt
    21001 gctttcacgc ggtctgcacc ctgatactgc gcgttgatct tctggaactc cgccagcatc
    21061 gggtcaacca cgtcaatggc attaccctgc tcatccacgc cctgggtgta acgcatccag
    21121 cccgccacgc ccagcgccag gtgacgccag ctaccgccgt tttgcaggtg cagacgcacc
    21181 gggtccagca gacgctgcgg taacttctgg ctgccgtcca ttgcaatctg ccaggtacgg
    21241 tgacgcagag acgggttgct gaaacgctcg atcagcagcg tcgcataagc gttcaggtct
    21301 gtaccttccg gcatcgacag cgttggcgct tgttcctgca tcatcagggc aaaggccgct
    21361 ttgcgataag ccgggttagt catggtgtcg gcaatagttt catagccgcc gaggtaaccg
    21421 aggtacgcca ggaaagagtg gctaccgttc agcatacgca gcttcatcat ttcgaacggt
    21481 acaacatccg caacaaactg cgcgccgact ttatcccagt ccggacgacc attaacgaag
    21541 ttatcttcaa ttacccactg acggaacggt tcgcaggcaa tggcgcacgg gtcgtaaaca
    21601 cccagctggt cagcaatttc ctgtaaggtt tctggcgtcg ccgccggaac gatgcggtca
    21661 accatggtgc acgggaaggt gacgttctct tcaatccatg ccgccagttg cgggtcacgc
    21721 gcctgagcca gcccaagaac ggcaaccttc gccacatgac cgttttcacg cacgttatcg
    21781 caggacatca ccgtaaatgc tttcagccct ttttcacgac gcagacgcag ggcttctacg
    21841 atgtaaccaa tcgcagactt cggcgcagtc gggttttcca gatcgtgctt gatcagcggg
    21901 ttattgagat ccagttgacc gcttgccgca tctgcgcagt agcctttttc cgtgaccgtc
    21961 agagagacaa tcgccgtctg cggacgcgcc atcgcgttga gaataccttc gcaaccgtcg
    22021 atttccgggt gcagggcttc tttcatcgaa ccgataattt tcagctcggt gctttctgcg
    22081 cctttttccg ccacggtgta cagcagttgc tgttttttca ggttttcgat cagcacgcgg
    22141 tcgttgcctg gcatcaggtt aacttcgcag atcccccagt cgctatccgt gctttccagc
    22201 aggtgatgag tgtacagcgc ctggtgcgcg cggtgaaacg ccccgcaacc gagatgcaca
    22261 atgcgtgatt ccagacgaga atgatcccat gacgggcggg caaccggcag attgctgtca
    22321 acaatagtag tcatcgtaaa ctccagaatc cgtcttcaga gagtgacggc aaggaagaag
    22381 gaaccggcac ggcgcgcagc catgccggtg gatattaacg gctaaagaaa gcgcgctgga
    22441 tcgccagttc gacaccgcga acttcggcca ggcctttcag acgaccaatt gcggagtaac
    22501 caggattggt tttcttcttc aggtcgtcca gcatctgatg accgtggtcc ggacgcatcg
    22561 ggatcaggtc ttctttgcct tccgctttac gacggtgttc ttcttcaaca atcgctttca
    22621 ccacttcgta catatcaacg tcaccgttca ggtgcgccgc ttcgtggaag gttttcgggt
    22681 tatcttcacg catggtggag cgcagatggg tgaagtaaat acgcggaccg aactgcttga
    22741 tcatatcaac cagatcgttg tcagcacgca cgccgtagga accggtgcac atggtgaaac
    22801 cgttcgccat gctgtttacg gtatcaacca tccactgcat atcttcaatg gtggaaacaa
    22861 tgcgcggcag gccgaggatc gggcgcggcg gatcgtccgg gtgaaccgcc atacgcacgc
    22921 caacttcttc agcaactgga ataatcgctt tcaggaagac agcgaagttt tcgcgcagct
    22981 tcgctttgtc gatatctttg tacagctcca ggtgcttacg gaactggtcg agggtatacc
    23041 cttcttccgc acctggcaga ccgacaatga tgttacgggt cagacgcgct ttgtcttcgt
    23101 cgctcatagt ggcgaagcga acagctgcct gagcaatttc ttcttcggtg taatccgctt
    23161 ccgcgcctgg acgcttcagg atatgcattt cgaatgcagc gaattcgatc tggtcgaagc
    23221 gcagagcttt ggagccgtct ggcagcacgt attcgaggtc agtacgggtc cagtcgagca
    23281 ccggcatgaa gttgtagcac acggtgcgaa tgccgcactg cgccaggttg cgcagggtct
    23341 gctgatagtt agcaatccac tgctcatagt tgccagtgtg ggttttgata tcttcgtgaa
    23401 ttggtacgct ttcaacgaca gaccacacca ggcctgcgtc ttcaacgatc gccttgcgtt
    23461 tgaggatctc ttctacggac catacttcgc cgttcgggat atggtgcagc gcggtaacca
    23521 cgccagttgc gcccgcctga cggacatcag ctaaagaaac cggatcgttt gggccgtacc
    23581 agcgccaggt ctgttccatg atttttcctc tttcacatag tgggttccac agcaaagaga
    23641 cgtggagccc cggagttgtt taggtatccg gatgaacaac cagaccgcag ccgtggcgtt
    23701 gcatcggatg agatggcgta taagttctac cttttggtaa gacatgtcaa cccgaaatct
    23761 gaaattggtt aaccacatca caagaatttc actctcctgg ttgttgctgc aaattgcagc
    23821 aaagggtaat cgatggttat tgatcagagg atgtgacatt catcgcaaca atggttgacc
    23881 aatttacata acatgtcgcc caaaataaca acggttcaac caggtaaggt aaaacgttat
    23941 gcatgtgctt aacattctct gggtggtatt cggcattggt ctgatgctgg tactgaattt
    24001 gaagttcaaa atcaattcaa tggtggcttt gttggtggcg gcgctgtccg tcgggatgct
    24061 ggcgggcatg gatttgatgt cgctgctgca caccatgaaa gcgggcttcg gcaacacgct
    24121 gggggaactg gctatcatcg tggtgttcgg tgcggtcatc ggtaaattga tggtcgactc
    24181 cggcgcggct caccagatag cacatacgct gctggcgcgt ctcggtctgc gctatgtaca
    24241 gctgtcggtg attatcatcg gcctgatttt cggtctggcg atgttttatg aagtggcctt
    24301 tatcatgtta gcgccgctgg ttattgttat tgccgccgaa gctaaaattc cgttcctgaa
    24361 actggcgatc ccggcagtag cagctgccac taccgcacat tcactgttcc caccgcagcc
    24421 gggtccggtg gcactggtga atgcttatgg cgcggatatg gggatggttt atatctatgg
    24481 cgtactggtg acgatcccaa gtgtcatctg cgcaggtctg atcctgccga agttcctcgg
    24541 caatcttgag cgcccaacgc catcattcct gaaagcagat caaccggtag atatgaataa
    24601 tctgccctct ttcggcgttt cgattctggt gccgctgatc ccggcgatca ttatgatctc
    24661 taccaccatc gccaatatct ggctggtaaa agatacccct gcctgggaag tggttaactt
    24721 tatcggttcc tcgccgattg caatgtttat tgcgatggtg gttgcattcg tactctttgg
    24781 caccgcgcgt ggtcatgaca tgcagtgggt gatgaacgct tttgaaagcg cggtgaaaag
    24841 cattgcaatg gtgattctga tcatcggtgc gggtggcgtg ctgaagcaga ccatcatcga
    24901 caccggcatt ggcgacacca tcggcatgtt gatgtcccac ggcaatatct cgccctacat
    24961 catggcatgg ctgatcaccg tactgattcg tctggcgacg ggtcagggtg tcgtttcggc
    25021 gatgaccgct gccgggatta tcagtgctgc aatccttgat ccagcaaccg gtcagctggt
    25081 tggcgtgaat ccggcgctgc tggtgctggc gacggctgcg ggttccaaca ccctcacgca
    25141 cattaacgat gcatctttct ggttgttcaa aggttacttt gacctgtcag taaaagacac
    25201 gttgaaaacc tggggtctgc tggagctggt caactccgtg gttgggctga ttattgtgtt
    25261 gattattagc atggtagcgt aaaagaacac cggagcctgc caatggcagg ctctgttcgg
    25321 attgtcggta aagtgcgctt tgtttatgcc agatgcgacg ctgacgcgtc ttatctggca
    25381 tacaaagggc taacgtgcag gttttagctt caggtaatat tgcgtacctg cattagcaat
    25441 gccctgtgat ttctttattg ataaacaaaa gtaacgccaa taatcgattg cacattccct
    25501 gcagtcacct gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa
    25561 gtccccactg ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc
    25621 aactgtacgc cgacgccctg tgctggtgaa aacgaggcgg tattggtgaa aatcgagttg
    25681 cccgcatctg cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa
    25741 acggtaagag gaatcggcac cgaaccggga tagtccggca gagtaacggt gacatcacga
    25801 gcagaaacat cacagccgcc agtaggcacc accacatcat tattggcgta aatattccac
    25861 acaaactgga aatcatcgct gttatagttg ttggtctgtc gcaaaattag cacggcaatt
    25921 aatgagccag ctttaatcgc caccccgccc gcactgctca caggcgtcaa ataaagcgcc
    25981 accggccacg gcttatccgt tctcgaatta taaacaacgc gcggcgtttc gctagtggtc
    26041 ggaaatggat agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg
    26101 ccataagccg agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta
    26161 tggcaaaaga tttgcgtcga aagatccacg accaggtttt gccccacatt cacggcaggc
    26221 gcaaggttta cataaacatt agcgctgcca ccgccaatag ggatagcggt gccattggcg
    26281 gttttacagg cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg
    26341 taaataactc gtttcattac aatcatctct tcggcttcag ctgtaggtat aggtgatgct
    26401 aattactgcc tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg
    26461 taacgggaag tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccact
    26521 attcaatgtg ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg
    26581 gtttttataa tatccggtac tgtcggctgc cccgctgaag ccggcggtga ccctcgacgt
    26641 tcccaccgga caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc
    26701 agacatcaga ctgaaagaat aaagatcgcc gagatctacc gtggcattgg tggtggaaac
    26761 cgtgcacggt ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat
    26821 cgtcgcactt gcgagcgcca acattgccgc caataaatac ccacgtttgc gccatttcat
    26881 gagcatctcc agttactgat attcaagagt gaaggtagcc gtggcattga tatgccccgc
    26941 agtgacaggc acctgtgtcg ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc
    27001 cggcgtcagg gtcgtccacg aaatcgcgga cgatggggca ttaaggggta tctgattttg
    27061 ttgctcattc agaagctgta ttcccagtcc cgcagccgct gacaccgtat tttcaagtgc
    27121 aagcaggttg gcattgtggc tatctgcaac accggtaaac ccaactttta cggcagaaac
    27181 ggcattacca cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat
    27241 gttgttaaat tgcttcaccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc
    27301 ggccacacta cagccattat ctctgacata gccgcggata gtaatcgtgc tatccgcagc
    27361 caaagcgtga cttaccgcca gccacaaaaa agcgcaaaga agataaaaag gtttgtttct
    27421 catcacgcct ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc
    27481 tggtggcagt tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg
    27541 aacttttccc gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc
    27601 gctactctgg ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg
    27661 ggttagcgtc atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc
    27721 cccacgggtg ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccagggt
    27781 attggtatcc agcgctactc tgttttcccg atattcagtg gcataaggca gcacggcata
    27841 accgcgccag tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg
    27901 cgctttaaca agcaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg
    27961 agccagtacc ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta
    28021 accgatgttg gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc
    28081 gctattacca tcacctcccc cggcgtaacc ggtttgcacg ctatagctga ggttgttgtc
    28141 ttccagcaac gtaccgtata caccggccag attggtcatc cgaccgttga gatcgtgtga
    28201 catgctgtaa ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg
    28261 gctgaaagga atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt
    28321 tttcgtcagg ctatagctga gcgtccagtt gatatcttcg aatgcagtat ttaaaccagc
    28381 ctggaattgc tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata
    28441 cagtgttgat gagcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt
    28501 ataagcgaga ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt
    28561 ttcgatgttg tagccattca ttcgactgta tgttgtatca gcgaaattaa aatacccact
    28621 ggtcgaataa cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt
    28681 ataaagaaaa cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc
    28741 ctgcgtcata tccacagaca gagcgcccag tgcccccata tttttcccga taccgaaatt
    28801 aaacgcacga taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag
    28861 gccgtggagt aatgtactct ggaaaaagcg gggtttttcc tgttgcgcat ttccactacg
    28921 gtattctcct gccgtaatgg aataacgtgt atgcccttca cgctgcaaaa gcgggactga
    28981 cgaatagggt acggtaaaaa tctgcgtact gccgtcagcc tctttaatcg ttacctgcaa
    29041 gtcaccacta ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt
    29101 actattataa atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat
    29161 accgtggatc accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc
    29221 caattgtgcg ccgcgaaagt taataccatc gaaaatatcg ccctgagtat aaccatcacc
    29281 cagcgtcagc cgggaacgta acggaattat gtctcgctca agccaggtat tgatatgctg
    29341 ccatttattt ttgctacctg atgaactgtc gctactgtta taactccagg tggtattgtc
    29401 gcgtaaacgc cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct
    29461 gttaccccca attcgattct gtacactatt tccgctgaaa ttataattga gcaatccggc
    29521 attaataccg ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa
    29581 tgcctgaggg atcgtcaggt tgagtcgctg ctgaccaaca tctaaatgcg cagtagcgtc
    29641 atggatcatt gaggttaatg gcacgcaggc atcatccgcc agcagattca taccggagac
    29701 agaagccgta ttcagcccca tactggcaag ttgcgcgcgt gtcaggcagg gaacaatccc
    29761 ttgttcactg tcgcccgtgt taaatgtgac atcacgcgtt gccatataac cattattcaa
    29821 atagatatcg acgcgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa
    29881 atcggccaca gcctggggat catccgctaa aaagcgcgga ttaaaataga gttcggcaga
    29941 tgacaaaggt gcctgtgcgg caaaagcaca ggcgacaaag agccggacaa aaaaaccagc
    30001 caaacgatgc ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag
    30061 atatgacatt attcctccgg tttaaaatca cccggcagtc aattcttttt attcaggcga
    30121 aaaattcccc ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc
    30181 atttattgtt cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc
    30241 gcccattgga ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac
    30301 cgtcaggtaa tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct
    30361 taatttttct gcggcctgat cgggtggcaa cgctaattta gccgggcgat agtacagctt
    30421 aatgcggctg ataattgcga gctgtagcgt attctcagtc aattttgatt tatccattga
    30481 cggaatcgct ttaacgttca tccagaataa actttcccga tcctgtggca attggttatt
    30541 tgttgcatca agaatacgta aggtattctc tctttttccc ttcatcgcaa acagaggtgg
    30601 cgtcacgata aaacgaccat cctttacacc atcggcattt tccacccatg attgaattaa
    30661 ataggtgcta ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg
    30721 ataaattacg cgggtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat
    30781 tgccatgaac atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac
    30841 attgacgttt ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta
    30901 ctgataggtt aaagagaacc aggcctgggc attggcgatg ccgccagtaa cccgacgccc
    30961 ggtagcacga tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca
    31021 gtttgctgga ggacgattaa tcggtacgag gtttccttca tcatcaaata acgctacgcc
    31081 aatattggtg gctatccctg gcccctctcc cacggaaagc acatccggat ttttaccatc
    31141 cgcgacaccg tgaaacgcca cacccacacg ttcactcacc accgtgctac attcccgtaa
    31201 atgaataaca aaaggcaccg gtgcgctatc ttcccctacc gcatgaaacc ggttactgct
    31261 gattttcccc atattgaccg tcatttgttt atcaccagct tcaactcggc aagtttccgc
    31321 aataatgacg ccctgaaatt gcatatttcc gccgggcagc gtggtattcc atttatttcc
    31381 ggccagagca aacattggca gcaacgacgc taatagaaat aatctcttcc ttttcatcgt
    31441 tatttttatc gcacaagggt gggcatccct gcccgtaatg acgtccctga acctgggtag
    31501 gttattgata ctgaaccttg aaggtcgcat ccgcattagc agcacccggg gttgcctcgc
    31561 cgattgcata ataacgcgcc tggaacggaa tggtgttggt accgttattc aaggttgttt
    31621 gtgcactaaa tgtcgcaccg tccagcgcca gcgcagcacc cgttctgtcc aggatctgca
    31681 caccaacttt tgttgcgcta cccgcagctg aactctgcaa agctaatacg ttaggatgag
    31741 ttccatcaat tgcggtacca aggaaggcaa tagcagcttt gtctgcaaca gtagtgtcac
    31801 aatcgttcag ttgaatgtta aaaccgacag cagaactggt tgctccttct tgtgccagcg
    31861 atgctgtacg aacctgccct aactgaacag tttgatcaac agagcctgca tcaactgcgc
    31921 aagcggcgtt aacaacctcc cctttaaagt gaacggtccc accatttacc gtcgtgggtg
    31981 tagtttcagc cagagccgct gtagaactca gggacagagc cgacagaaca acaattgcca
    32041 gagttttaat tttcatactg ctttcctttc aaaaaactat ttctaaatcg acatgggcag
    32101 tcgttctgta cactttgttt tgtcaacgag tttgactgcc aacactgcac agttttcccc
    32161 caaaagatga aacatttggg gccattttga ctcatagagg aaagcatcgc gcacaaactt
    32221 tttcagttta tttgttggct taatattcta ttgttatctt tatttataga tttttatatt
    32281 gcatgaggtg gtttttgtag agaagaatga ggaagatgcg tcgagccaca gaaa
//
LOCUS       DAZKJR010000045        32199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKJR010000045 DAZKJR010000000
VERSION     DAZKJR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.045"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(520..1350)
                     /locus_tag="ACHOWS_002102"
     CDS             complement(520..1350)
                     /locus_tag="ACHOWS_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFQ0047673.1"
                     /translation="MKLESSLKHFSPRGARIINDVKETSPERLTGTDVMAAIGTTSCR
                     TRFGLAAFFGKAGLSKSDEQLAVQVLARYAMDTAPKNVRKAAGSEFGWCMLVLAQFAF
                     AEYSRSAATSVACPKCSGTGRMECEQLTRKVSYPWGKAPYWASRSRAVRPSDWEKWTE
                     VTEVVPVVCDVCKGKGVISARCRCGGKGEVLDRKATSERGAPVFKICERCSGNGFSTV
                     PSTAAYKVVLKRIPELHVRTWTRNWKPFLDSLVDICHQEERKADIAFQNVTSFGDDVN
                     "
     gene            complement(1386..1580)
                     /locus_tag="ACHOWS_002103"
     CDS             complement(1386..1580)
                     /locus_tag="ACHOWS_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFQ0047674.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCSRNTIRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            complement(1577..2188)
                     /locus_tag="ACHOWS_002104"
     CDS             complement(1577..2188)
                     /locus_tag="ACHOWS_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFQ0047675.1"
                     /translation="MAKPARRRCKNNECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            complement(2181..2390)
                     /locus_tag="ACHOWS_002105"
     CDS             complement(2181..2390)
                     /locus_tag="ACHOWS_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFQ0047676.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCADCCAELMSDPNSSMYEEEDDG"
     gene            complement(2350..2751)
                     /locus_tag="ACHOWS_002106"
     CDS             complement(2350..2751)
                     /locus_tag="ACHOWS_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047677.1"
                     /translation="MLLIQPRFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            complement(2754..2930)
                     /locus_tag="ACHOWS_002107"
     CDS             complement(2754..2930)
                     /locus_tag="ACHOWS_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFQ0047678.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKQIPKESDVKTFNYTA
                     HLWDIRWLRERARK"
     gene            complement(2927..3367)
                     /locus_tag="ACHOWS_002108"
     CDS             complement(2927..3367)
                     /locus_tag="ACHOWS_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFQ0047679.1"
                     /translation="MRKLTFELRSTIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(3348..3512)
                     /locus_tag="ACHOWS_002109"
     CDS             complement(3348..3512)
                     /locus_tag="ACHOWS_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047680.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            complement(3515..3805)
                     /locus_tag="ACHOWS_002110"
     CDS             complement(3515..3805)
                     /locus_tag="ACHOWS_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047681.1"
                     /translation="MDESRKQFEESWLRRGGESSYLIRYPENHHEIGSGDIGGQYVMD
                     DVQGHWQTWQASRAAIEIELPESFTMHSGRTPYLYVSEVQSAIRAAGVKVKE"
     gene            complement(3817..4065)
                     /locus_tag="ACHOWS_002111"
     CDS             complement(3817..4065)
                     /locus_tag="ACHOWS_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012987735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047682.1"
                     /translation="MTYPHILEIHEKAAAINAGRKPQFGDKMRNLVAGEDNPRRDAYF
                     VKQKRVTGRMNPGLWYTMTERKGNFWDSCPKGLIFIDE"
     gene            complement(4448..5884)
                     /locus_tag="ACHOWS_002112"
     CDS             complement(4448..5884)
                     /locus_tag="ACHOWS_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFQ0047683.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKILFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTAMQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDMLYIDDTPGLSLTQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            complement(5874..6773)
                     /locus_tag="ACHOWS_002113"
     CDS             complement(5874..6773)
                     /locus_tag="ACHOWS_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFQ0047684.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTSKPAIPMPASNTVPADYQQ
                     ITTDKEYNNIISNTDVLESTAADKKSDKKKPSVSCQDVVDAYHEILPEAPRIRALNDK
                     RKNQIRTFWRKAGVITRQLDGHGFTVQDWRNYLSYVGENCRWMFEERPNHQRGTVWHK
                     KGFDFLLNDNTYLKVREGEHDDR"
     gene            complement(6766..6912)
                     /locus_tag="ACHOWS_002114"
     CDS             complement(6766..6912)
                     /locus_tag="ACHOWS_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HFQ0047685.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            complement(6945..7238)
                     /locus_tag="ACHOWS_002115"
     CDS             complement(6945..7238)
                     /locus_tag="ACHOWS_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFQ0047686.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            complement(7358..7573)
                     /locus_tag="ACHOWS_002116"
     CDS             complement(7358..7573)
                     /locus_tag="ACHOWS_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0047687.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVECLNKLGAKVSLDDVFPPEHKAA"
     gene            7649..8344
                     /locus_tag="ACHOWS_002117"
     CDS             7649..8344
                     /locus_tag="ACHOWS_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFQ0047688.1"
                     /translation="MDGFSTERNNKGMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLDEIGVVFKYLGIDNVSFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERWVSTTKKASDCAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNDSCSVVG
                     KVIASQWPEETFS"
     gene            8715..9074
                     /locus_tag="ACHOWS_002118"
                     /pseudo
     CDS             8715..9074
                     /locus_tag="ACHOWS_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
     gene            9083..9532
                     /locus_tag="ACHOWS_002119"
     CDS             9083..9532
                     /locus_tag="ACHOWS_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047689.1"
                     /translation="MEEQANKILVELLQKASNGIDAAVSFSQAQIPDVVHQLLLWNMV
                     DSLIKTLIAILTIPLVFWFMKKQCQRVETGKIGDEGYSWERGNPKYRPTMVWDSKGDI
                     NLLIMPLVGVLTLWGIFIIGVVTNMTWLKIWLAPKLYLIEYAASLVK"
     gene            9586..9912
                     /locus_tag="ACHOWS_002120"
     CDS             9586..9912
                     /locus_tag="ACHOWS_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047690.1"
                     /translation="MKVLMVYENVPESTEIYIFDANEDEVNDLKLSHGNYTNANCDES
                     IEKALSRVLVRISDPEHCDDDWLSYCGAVKTDAGKWSKSKVDNSTPIIMKDSDIEMVI
                     ITGMIM"
     gene            10109..10279
                     /locus_tag="ACHOWS_002121"
     CDS             10109..10279
                     /locus_tag="ACHOWS_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047691.1"
                     /translation="MQQFAIAGAASVRPFNPILSVQHSRKNILTGADFKQPRVKSLLD
                     RLVEFLNQKVQP"
     gene            10276..10440
                     /locus_tag="ACHOWS_002122"
     CDS             10276..10440
                     /locus_tag="ACHOWS_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047692.1"
                     /translation="MKKPTYEELEEALKELRRMAFARRTNSHNCGPFQYSDLCEDIIE
                     VTQLIKVVKQ"
     gene            10525..10833
                     /locus_tag="ACHOWS_002123"
     CDS             10525..10833
                     /locus_tag="ACHOWS_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047693.1"
                     /translation="MILTLNDKREISQIIASFTDEDYERINSEVDRLCKRCDPISEML
                     RLYKPDEHTKDAIDWLEDDDCNYQEKAAEWFWDAITERVKAEYAFAIFKRRHIYGEAA
                     "
     gene            10830..11732
                     /locus_tag="ACHOWS_002124"
     CDS             10830..11732
                     /locus_tag="ACHOWS_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0047694.1"
                     /translation="MSNIVEFVKQQEQLFCGALTEQTVTWAKESQFAIQYFQKNDYLA
                     KTALANPTSAQNAIINVAAIGITLNPASKLAYLVPRDGMVCLDISYMGLLHLAQSTGS
                     IKWGQCKLVYSNDTYESNGLDSAPTHKYNAFGERGSIVGGYCTVKTADGDYLTEEMSL
                     AEIKAVEATSKAKNGPWKTFWEEMARKTIVKRASKYWPKAQRLDNAIHLLNEDEGMHQ
                     EPVMPHKSEEDIREDERKRQQEITDKAQLLCDEMAQAENMDDLKRYFAEAYRLTSGMK
                     LQQNIQAIYIECKAKLEVASEQTI"
     gene            11716..12198
                     /locus_tag="ACHOWS_002125"
     CDS             11716..12198
                     /locus_tag="ACHOWS_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0047695.1"
                     /translation="MSKLYEIANEYAKLMDSDLEPEMIADTIEGMEGEFTDKIEQLLA
                     IIKNESGYAERLKEEAKSLNERAAVIQNKIDSIMAYIASSLEMVGKKKIRAGIHQVTI
                     RKPSETVEIIDSSAIPPEYVEFETTIKADKLAIKHQLKAGINIPGAQLKVGKPSLLIK
                     "
     gene            12210..12524
                     /locus_tag="ACHOWS_002126"
     CDS             12210..12524
                     /locus_tag="ACHOWS_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047696.1"
                     /translation="MKKTPWEKWEVDFLREVAATMPVEVIAEKLERTEKAVMAKATRI
                     GADIVSRLRGRRWTRAEVSLFGKFSAEEIAIATCRSIYSVRAMRYKLKKLDEERAGIR
                     IN"
     gene            12541..12705
                     /locus_tag="ACHOWS_002127"
     CDS             12541..12705
                     /locus_tag="ACHOWS_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFQ0047697.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRSGKK"
     gene            12702..12902
                     /locus_tag="ACHOWS_002128"
                     /pseudo
     CDS             12702..12902
                     /locus_tag="ACHOWS_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12921..13271
                     /locus_tag="ACHOWS_002129"
     CDS             12921..13271
                     /locus_tag="ACHOWS_002129"
                     /inference="COORDINATES: protein motif:HMM:NF025303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047698.1"
                     /translation="MKSAIPESRDIEDDNDNMDDVSLAEDFGFNHAIERMRRQIPETP
                     TTDAFLAEVRAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLVGKVEMTEW
                     LVDFAAQLRKGGSQ"
     gene            13268..13993
                     /locus_tag="ACHOWS_002130"
     CDS             13268..13993
                     /locus_tag="ACHOWS_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFQ0047699.1"
                     /translation="MSKIDYQALRELAKQATQGEWVAFISTGTGTYAVHTPGDKRCED
                     VIKWTGFDGQKNAENNARYIAAFNPEVVQALLDENESMCRDLIARNGEIEGLRKLVAE
                     LEETKSKLNEQREYYEGVISDGSKRIAELEKSEEQLINERDHAESALADMYFAATGDR
                     PEWSNCFSFSDAVDAVVDRIADLEAKQASPVVPEELIKAVRFYEQVKRENPSVETGAW
                     KDAVDWVLKEACQAVSISNKGGE"
     gene            complement(14087..14341)
                     /locus_tag="ACHOWS_002131"
     CDS             complement(14087..14341)
                     /locus_tag="ACHOWS_002131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047700.1"
                     /translation="MRKWFFQRFCAALGFLQQLCPITPQHPVNLTRLLLILNHRQTPT
                     PFLRANRIFALRFFIVLLSCVSLLPVSPPQYLYGGALVPG"
     gene            14356..14958
                     /locus_tag="ACHOWS_002132"
                     /pseudo
     CDS             14356..14958
                     /locus_tag="ACHOWS_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245496.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            14951..15235
                     /locus_tag="ACHOWS_002133"
     CDS             14951..15235
                     /locus_tag="ACHOWS_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0047701.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYEGYEIKTITHTHFGDKPVKVFAIKVNIGSD"
     gene            15284..16024
                     /locus_tag="ACHOWS_002134"
     CDS             15284..16024
                     /locus_tag="ACHOWS_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047702.1"
                     /translation="MINNIANQSSFPAATIDSQMLLKMVNEARKLCSEKPVRNNDFIA
                     RVKDELEGETYEIFVGQKNGAEIEVITMTFKQALRVAARESKAVRRSLVDKLESMQQQ
                     LQQKTTTKKSPDGLEEFRKARALKMTVDTMKDLFDFLPNLAPEAKQVVAASLVNPVVG
                     ANVIPLPMINEHYYSASEVGAKLKISANKVGRIANTYMLKTEQYGKWFIDKSPYSDKQ
                     VESFRYNNRGVRKIEEILDAENKAEFGT"
     gene            complement(16067..16372)
                     /locus_tag="ACHOWS_002135"
     CDS             complement(16067..16372)
                     /locus_tag="ACHOWS_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFQ0047703.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NTTISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(16462..16734)
                     /locus_tag="ACHOWS_002136"
     CDS             complement(16462..16734)
                     /locus_tag="ACHOWS_002136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047704.1"
                     /translation="MSLAAPIRDPIMNIIARFFYLIIFCMATSGCTTTTKINRGDEKE
                     QYIIACGAATPWGLCYDKANALCKNGYKDILKEQGFNRKELTIECK"
     gene            complement(16788..17045)
                     /locus_tag="ACHOWS_002137"
     CDS             complement(16788..17045)
                     /locus_tag="ACHOWS_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFQ0047705.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAMEAWLIHSAAPPLDAIRENLGKGHSISQVRSEIRARK"
     gene            17370..18047
                     /locus_tag="ACHOWS_002138"
     CDS             17370..18047
                     /locus_tag="ACHOWS_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFQ0047706.1"
                     /translation="MTTQVSVETLSTITYKQIPVITTELLAHLYGTEAIRIRQNHHEN
                     KGRFIEEKHFFKLEGETLREFKHRVAFNYSVKIARNVRSLILWTERGAARHAKMLETD
                     RAWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSEHHPYSMMSYVDRKTLNLD
                     VSVLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSR
                     KGFSLSL"
     gene            18103..18534
                     /locus_tag="ACHOWS_002139"
     CDS             18103..18534
                     /locus_tag="ACHOWS_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFQ0047707.1"
                     /translation="MKQIAFYRRSGRPGAFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            18531..19157
                     /locus_tag="ACHOWS_002140"
     CDS             18531..19157
                     /locus_tag="ACHOWS_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFQ0047708.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            19117..19329
                     /locus_tag="ACHOWS_002141"
     CDS             19117..19329
                     /locus_tag="ACHOWS_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFQ0047709.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARRGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSPTV"
     gene            19364..19762
                     /locus_tag="ACHOWS_002142"
     CDS             19364..19762
                     /locus_tag="ACHOWS_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFQ0047710.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIASVQPVQPVQPVQPVQPVQPVQHNIIGDLLRKSRKEARR
                     AGRKQKRWEGACKALQELNKYRDLINELSE"
     gene            19906..20139
                     /locus_tag="ACHOWS_002143"
     CDS             19906..20139
                     /locus_tag="ACHOWS_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047711.1"
                     /translation="MKITFESYGLTASVVISSSIFESRKHQHIVDAVKLKAPEVTVTT
                     HGLFRIRTTLTTTHLSACRVYDMALKEYNQCSS"
     gene            20127..20378
                     /locus_tag="ACHOWS_002144"
     CDS             20127..20378
                     /locus_tag="ACHOWS_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFQ0047712.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            20439..20621
                     /locus_tag="ACHOWS_002145"
     CDS             20439..20621
                     /locus_tag="ACHOWS_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0047713.1"
                     /translation="MEINLMTEKEVSELLQKRRTALYNLRKKHGFPDPVLTHPARYSR
                     QAVEQWLKSGGINRAV"
     gene            complement(20605..21774)
                     /locus_tag="ACHOWS_002146"
     CDS             complement(20605..21774)
                     /locus_tag="ACHOWS_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0047714.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVSGLKPNTQTLYRSVVYNTMYTQFEDSPVAGIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLTLENS
                     KVVTSNKLLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPIFTQ
                     VEPYIERLLNIGFDVLFPGQEIDKPIDRSSANLYMKKLRAKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            21970..22044
                     /locus_tag="ACHOWS_002147"
     tRNA            21970..22044
                     /locus_tag="ACHOWS_002147"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22003..22005,aa:Arg,seq:cct)
     gene            22224..22406
                     /locus_tag="ACHOWS_002148"
     CDS             22224..22406
                     /locus_tag="ACHOWS_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFQ0047715.1"
                     /translation="MWRDSILHSAMHSLMNSQARDTRDTSLNDDNVLEISEPRIRSTF
                     ERLYQNMNEAFGLGDY"
     gene            complement(22486..23733)
                     /locus_tag="ACHOWS_002149"
     CDS             complement(22486..23733)
                     /locus_tag="ACHOWS_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0047716.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(23805..24719)
                     /locus_tag="ACHOWS_002150"
     CDS             complement(23805..24719)
                     /locus_tag="ACHOWS_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFQ0047717.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDWDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            24935..26368
                     /gene="cscA"
                     /locus_tag="ACHOWS_002151"
     CDS             24935..26368
                     /gene="cscA"
                     /locus_tag="ACHOWS_002151"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0047718.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDSGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(26376..27371)
                     /locus_tag="ACHOWS_002152"
     CDS             complement(26376..27371)
                     /locus_tag="ACHOWS_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0047719.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHISLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDREGGRVTRIPCPLLIRCST"
     gene            27614..27832
                     /locus_tag="ACHOWS_002153"
                     /pseudo
     CDS             27614..27832
                     /locus_tag="ACHOWS_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            27850..29181
                     /gene="dsdA"
                     /locus_tag="ACHOWS_002154"
     CDS             27850..29181
                     /gene="dsdA"
                     /locus_tag="ACHOWS_002154"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFQ0047720.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLTQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(29286..30824)
                     /gene="emrY"
                     /locus_tag="ACHOWS_002155"
     CDS             complement(29286..30824)
                     /gene="emrY"
                     /locus_tag="ACHOWS_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFQ0047721.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(30824..31987)
                     /gene="emrK"
                     /locus_tag="ACHOWS_002156"
     CDS             complement(30824..31987)
                     /gene="emrK"
                     /locus_tag="ACHOWS_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFQ0047722.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTTPIEKEISNIISHNGQL"
BASE COUNT         8970 a         7517 c         7385 g         8327 t
ORIGIN      
        1 ttgctgcttt gacgctacgg acataaaaaa gccagccact gggggaggct ggcaaactcg
       61 tagagcaaaa tgctgttacg caaacttcgt tacagggtta tcctgcaata cttaaaatat
      121 acaatattta gaaaactaat agtgccacat gcgattttta agattttgtt attaattacg
      181 gtcgcatctt cctttctgtg tactttccgt atagctcaca ggattctggg tacaaaaaaa
      241 cccgcgcatc ggcgggttcg gctgcgtggc aatgtaacca ctcttatcat gatatgcaga
      301 tttttacgat cgtaaactat tttttcgctg ataaaatact catcaacagc aaacgagatt
      361 gagcttcgtg atttcgaaag tttttttagc caattgaagt aaaaaagccc ccttgagggg
      421 gctttctgtc aaaatcatcc aggcgttatt ccgcattatc aataatgata catgacagat
      481 tcggacaaaa tcaagtgcta tatcgcaaaa agcctaagtt tagtttacat catcaccaaa
      541 acttgtcaca ttctgaaaag caatatcggc cttacgctct tcctggtgac aaatgtcaac
      601 cagagaatca agaaacggct tccaattacg tgtccatgtc ctgacgtgta gttcgggtat
      661 ccgcttcagt acaactttat atgctgcggt agacggtacc gtagaaaatc catttccgct
      721 gcaacgttcg cagattttaa acacaggcgc accacgctcg cttgtggctt tgcggtcgag
      781 tacctctccc tttccaccac aacgacacct ggcacttatc actcccttcc ctttacatac
      841 atcgcaaacg actggtacaa cttccgttac ctctgtccat ttctcccagt ctgacggtcg
      901 aacagcgcgg gatcgactgg cccaatatgg tgctttaccc catgggtatg aaaccttacg
      961 tgtaagctgc tcgcattcca ttcgcccagt accactacat ttggggcatg ccacactagt
     1021 agcagccgat cgggaatatt cagcaaaggc gaattgtgcc agtaccaaca tgcaccagcc
     1081 aaactcactc ccggcagcct tccttacgtt ctttggagca gtatccatcg catatctcgc
     1141 cagcacctga actgcaagct gttcatccga tttactgagc ccagctttac caaagaaggc
     1201 tgccagaccg aaccgggtcc tgcaactagt ggtaccaatg gcagccataa catcggttcc
     1261 agtgagacgt tccggggaag tttctttcac atcgttgata atgcgcgccc ccctggggct
     1321 aaaatgtttt agagatgact caagtttcat ttttcccctt ccgacgattc agaagctgct
     1381 gaatatcacg catcagtatc tttaccccat ccgcgatgaa ccataagaac accgttgacg
     1441 atggcgtgtc ttttcccttc tttatcgcca gtgtattttc tgatcgtgtt gcgactacag
     1501 ttcagtattc tggctacctc ggtctgattt ccatatgcct caacgagcat gtcaggaatg
     1561 gtttttactg tgaacgtcat gcggcctcac ttctgctgtt tcgcaggtct ttaagtttct
     1621 gctgatactt cgccttgatc gccctgcatt cttcgacagt ccagcgatgg cggttatggt
     1681 tcgattcgat ttcgtctact gcttcctgcc cgatgcggtt aatcagttcg acgcgatacg
     1741 gaacgagatt tccgcttttg tactggttac acaccacgca ttgcttgtga atattgcgtt
     1801 catcaaatcg gagttgaggt gccgcagcag ttgtccggta atgtccggca tcccactgag
     1861 cagacgtgag cgttccgcac gagatacatg gtaagtcgcg gtctctttct ctgatgaagg
     1921 cgtttacggc ttgttgggct tgtttaatcc agtaactgcg gggctttaag gcgagttttc
     1981 gaatcttaag tttatctttc tgtttctgct cctctcgtcg tcgtttcttc tctgctgctt
     2041 tttccgcttt ttcgcgttct ttacttcgtc gttcgagtgc tatcttggtt ccacactctg
     2101 gagagcacca ccactgatta gcgaatgcag ggtgaaacca ttcccgacat tcattgtttt
     2161 tacatcgtct tcgcgctggt ttagccatcg tcttcttcct cgtacattga gctattcgga
     2221 tcgctcatca gttctgcgca gcaatcggcg cacacgtgaa cttccagcac gtgcagcttc
     2281 tgaccgcagt tagcgcacgt taaagcccgc tcgacgcttt ctttctggta ttgaagagat
     2341 tgggatggac taagcatggc tttcaccatt aaaaagtcgc ttgtaagcat caatgtctcg
     2401 ttttgcttca ccaagctttc gtcttaattc catgttttct gattcaagct tttccatgtc
     2461 ttgctggtat cgatcgcggt gttctttcca tgcttttcga tacgccttca tgtatgtcgt
     2521 attggccttt ctctttgcct gacgaactgc gtggtggttt ttcacaaacc agtcagggtc
     2581 gttaaatgct gctctggcgc atgtatacca ataatttgtt gcctccctgt ttagccaata
     2641 aatactgata aatggcaacc ggatagacac catttttcgt tgtgactctt tctcaccaaa
     2701 catgtgccct tttttgatgc taaggccaaa tctaggttga attaaaagca ttgtcatttc
     2761 ctcgcgcgtt ctctaagcca ccggatatcc cacaggtgag ccgtgtagtt gaaggttttt
     2821 acgtcagatt cttttgggat ttgcttgcgt ttatttctgg agcgtttcgt tggaaggtat
     2881 ttgcagtttt cgcagatgat gtcggtgata cttcgtcgct gtcgtctcat gctgccctgt
     2941 ctccccatcg cgctttccac tccagagcca gtcgcgcttc gtctgaccac ttaacgccac
     3001 gctctgtacc gaatgcctgt ataagctcta atagctccgc aaattcgcct acacgcatcc
     3061 tgctggttga ctggcctatt accacaaagc cattcccggc aaggttagga acaacatcct
     3121 gctgctttaa tgctgcggta aacacacact tccagctttc tgcatccagc cagcgaccat
     3181 gccattcaac ctgacgagag acgtcaccaa ggcaagccca aagctttcgg ttttggtcta
     3241 agctacggtt tcgttcctgg atggtcacta cgattggttt ggttgggtct ggaaggattt
     3301 gctggatagc ttgaatggcg ttctgctgat ggatggtgct tcttagttca aacgttagtt
     3361 tcctcaccat ttacgctcct gtaatcgtca agtgcagctg caatagtccc tatcgggtca
     3421 tggtcttgcc cgataatctc atttacgttt tcatcttctt ccacaccgaa aaagaatcgc
     3481 agggctaaca tgatttcttc gtatgcgctc atactcactc cttcactttg actccagcag
     3541 cgcggatggc gctctgcacc tcgcttacat acagatatgg ggttcgccca ctatgcatgg
     3601 taaagctttc aggcaactca atttcaatag ctgctcgcga tgcctgccac gtttgccagt
     3661 ggccttgaac atcgtccatc acgtattgac caccaatatc accactgcca atttcatggt
     3721 gattttcagg gtaacggata aggtatgaag attcgcctcc acgtcgcaac caactttctt
     3781 caaactgctt tcttgattcg tccatattcc tctccatcac tcgtcaatga aaatcaggcc
     3841 tttcggacag gagtcccaga agttaccttt gcgttctgtc atcgtgtacc agagcccggg
     3901 attcattcgt cctgtcactc ttttctgttt cacgaagtag gcatcgcggc gcgggttgtc
     3961 ttcaccggcc acaaggtttc gcattttgtc cccaaactga ggctttcgtc ctgcgttaat
     4021 cgctgccgcc ttttcgtgaa tttctaaaat gtgcggatat gtcataattc ctctccatca
     4081 gcgtgctggg gtgttaagcg tcgtcgccct gaaccatcac gtactgaccg catgagtcgc
     4141 agcagtactc attcatgctt cttccatcat ctgtccagtt atcgcttccg catttgttgc
     4201 aaaggagttt tctaaactgc ggcggagtta ttcttacctt tccgaaatta gttacccatt
     4261 gagttgcatc gtcgaatcta taactattaa ctttcatttt tctgtgcccc caaatagacc
     4321 tatattttaa ataaaggtca ggagacataa tcggctcgct cacactctca cctctcgctt
     4381 ccgatccaat aaaaaagggc tactgtgtaa atagcccctg ttattagctc agtgatgtag
     4441 atggtcatca gaatcctcct ttcttcttgg attgcggttc ctcgcgttca cggcggcgca
     4501 tttcagcgga ctgttggtct gtgtcataaa tagcgccatt tgcctgaatg caatacaccg
     4561 tgccggtatt gccatgacga ttgagacgaa ggattagttc agtttcacca ggtggaacac
     4621 tgtcatcaaa agcgccttca cgatggatcc cgacccaata atcgcaatcc tgttcaatct
     4681 gccctgtatc tcgtgagtca cttggtaatg ggcgtttatt ggttcggctt tccagtgcgc
     4741 ggttaagctg tgtcagaagc acaacaacgc aatcaagctc tttggcaagg ttcttcagtc
     4801 ctttggtgat catgccgtaa gcaaggtcgt tgcgatcggc cttctcagcg gtcattagtg
     4861 tcaggtaatc gaccagaatc atgccaacac atcctttttc tcgcttgatt cgacggcttt
     4921 cgctgacgat ttgagtcaga gataatcccg gcgtgtcgtc gatgtaaagc atgtcgattt
     4981 cactcaagcg atttgctgtt tctatcgccc tgttgaagtc accatcgtaa tcaccctgat
     5041 agccgtcatc agcgtcattt gtcgccggaa ggtaaaaaat attcggatta acaccagact
     5101 tctgccctac cagtttttcc agtatctgat cacctggcat ttcaaggctg aacatcagag
     5161 cgggcttttt ctcatgcact gcgcagttga ttgccatctg gctgtatagc gtcgttttcc
     5221 ccatcttagg gcgagcgcca atgacaaaca gagagccttt caccagacct ttcggtgaca
     5281 gcatcctgtc cagcgatggg atccctgtgc tcattcctcg ttgttcgcct gacgggtcaa
     5341 atcgcttctc aaggtcgcta acccagtctt ccatgacctc accaaatgag cgaaggccgc
     5401 gacgcgatcc ggtttttgca tggtctgtca gttgcgtgaa aatcgcctga atagcttcgt
     5461 acttctgcat tgcagtcatt ccgttgcggg aatagagcaa ttccgtcgct tcagtcatgc
     5521 ggttgatggc gtagcgttcc attgcggttt cacgaacctg cattgcatag gcaacgatgt
     5581 ttgctgcgct tggagtgttc tttgcgatct cagcgatata agcaaaaccg ccaacagacg
     5641 ccgttaacga tttacgctcc agttcatcga aaagcgtcag gccatctacc ggcttttgct
     5701 cccggtgcat tctggttatt tcttcgaaaa ggattttgtg tggtcggctg taaaatgaat
     5761 cgggcttcag catcgccaga actttctgga cgcgctcact gctgtcatca tccagaagca
     5821 atccaccaat caccgcctgc tctgcctcga tgctatgggg cggcgcataa aaattatcgg
     5881 tcatcgtgtt caccctcacg aactttcagg taggtattat cgttaagcag gaaatcaaat
     5941 ccctttttgt gccagacggt tccgcgctga tggtttgggc gctcttcgaa catccatcgg
     6001 caattttcgc ctacgtagct caaataattt ctccagtcct gcaccgtgaa cccatgcccg
     6061 tcaagctggc gggttatcac tccggctttg cgccagaacg ttcggatctg gtttttacgc
     6121 ttgtcattca gtgcgcggat tcttggcgct tcaggaagga tttcgtggta agcatcgaca
     6181 acatcctgac agctgacgga aggttttttc ttgtcagact ttttgtctgc tgcggtactc
     6241 tctaatacgt cagtattaga gataatatta ttatattctt tatctgtggt aatttgctgg
     6301 taatctgctg gtacagtatt gcttgcaggc attggtattg ctggctttga ggtggtgatt
     6361 tgctggtaat ctgctggtac aaaatttgac tgataatcgt catatttctc taccgagaaa
     6421 actgagaatt taccgtgtga aacccagtca atcatgccga gttttttgaa ctttctaagc
     6481 aggtactgaa cgcgatctgg tttgagtcct gtttcaaacg ccagagagtt tctaccgcca
     6541 agtagcttcc ctctgcctac cagaatttct cctgcgtcag tcattacata ctcaggcgta
     6601 tgctttgctt tgaggattaa gtgaacccac agatgcgcag cttctgcgtc cttgtaaaac
     6661 ggcacatcca taattttacg gtgcagcaag gcatacccct taccgctgct ttgatgcggt
     6721 tgttgtagcc ttctggcctc tctggcttcg gctagattag atatgttact catgaccttt
     6781 ctccttctgc atcagcttca ctttttccaa ctcagcccgg aatcgaccag gctgcttgaa
     6841 gctggacagg aagcgatcac gtagtatgtg tttgtgaatt ttgtcctggt aaggactgag
     6901 ttgttttgtc ataatgactc ctgtggattg atccagtaat gacctcagaa ttccatctgg
     6961 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat cgaagcaact
     7021 tgtcgtgcca atcgagccat gtcgtcgtca acgacacccc attcaagaac agcaagcagc
     7081 attgagaact ttggaatcca atccctcttc cacctgctga tctgcgactt atcaactccc
     7141 acagcttccg ctgtcttctc agttccaagc attgcgattt tgttaagcaa cgcactctcg
     7201 attcgtagag cctcgttgcg tttgtttgca cgaaccatat gtaagtattt ccttagataa
     7261 caattgattg aatgtatgca aataaatgca tacaccatag gtgtggttta atttgatgcc
     7321 ctttttcagg gctgggatgt gtaagagcgg gaatgtctta agcggctttg tgttccggcg
     7381 ggaacacgtc atcaagactg acttttgcgc ctaacttgtt taggcactca acaagagcac
     7441 ggcatgtttt aaggtctggg aagcgacgac cagattccca atgtccgata gctccctgtg
     7501 tgcatccaac tgccttagca agtgttgttt gagagatatt cagtgactct cgatattttc
     7561 gtaggttgct catatgccct ccatagtaac catgaaacaa taatacgata tgtactttta
     7621 gaatgcaaac aaaaaataca tcttgtgcat ggatggtttt agtacagagc gtaataataa
     7681 gggtatgaaa atgaaatggt atgaactggc tagatccaga atgaaagagc taggcataac
     7741 tcaagagaag ttagctgaag agcttggtat gacgcagggt ggaattggtc actggttgcg
     7801 cggatctcgt catccatctc ttgacgagat tggtgtggtg tttaaatacc ttggtattga
     7861 taacgtctca ttcaaccacg acggtacatt ttcacctgtt ggcgaatact catctgcccc
     7921 cgttaaaaaa caatatgagt accctgtttt ttctcatgtt caggccggga tgttctcgcc
     7981 tgagcttaga acctttacca aaggtgatgc ggagagatgg gtcagcacaa ccaaaaaagc
     8041 cagtgattgt gcgttctggc ttgaagttga aggtaattcc atgaccgcgc caacaggatc
     8101 caagccaagc tttcctgacg ggatgttaat tctcgttgac cctgagcagg ctgttgagcc
     8161 aggtgatttc tgcatagcca gacttggtgg tgacgagttt accttcaaga aactgatcag
     8221 ggatagcggt caggtgttcc tacagccact aaacccgcaa tatccaatga ttccatgcaa
     8281 tgatagctgt tccgtagtag ggaaagttat cgccagccag tggcctgaag agacatttag
     8341 ttaacagcct cacaactcta aaatacacaa caataacccg accttagcgt cgggttttct
     8401 ttttccaaaa tataaaccca ttaaatacaa agcgttataa aaaactaatt atatttagaa
     8461 cattttgtat tgactcaata aagtacaaat cgtactattt agccatcagc aggacgcact
     8521 aaccaccatt gaaggtgagg ctcttaaaaa tttagccctg aagaagggca gcattcaaag
     8581 cagaaagctt tggggtgtgg tgaagggttc atggatggga atatgtcgca cgtaaagcgg
     8641 cgaggcctgc ggaactattg ccgaattgaa gtcggccgaa gcaggtcgaa atgggtctcc
     8701 cacctaccac accaccaaag ctaactgaca ggagaatcca gatggatgca caagcacgcc
     8761 gccgcgaacg tcgcgcagag aaacaggctc aatggaaagc agcaaatccc ctgttggttg
     8821 gggtaagtgc aaaaccagtt aaccgcccaa ttctctcgct gaatcgcaaa ccgaaatcac
     8881 gagtagaaag cgcactgaat ccgatagacc ttacagtgct ggctgaatac cacgaacaga
     8941 ttgaaagcaa cctgcaacgt attgaacgca agaatcagcg cacatggtac agcaagccac
     9001 gcagtgaaat gggtgtgacc tgttcaggcc gccagaagca acgcggaaaa tcaattccag
     9061 cttattacga ttgaggtgag atatggaaga acaagcaaac aagattctcg tagaactact
     9121 gcaaaaagcc agcaatggaa tagacgcggc tgtttcattc agccaagcgc agattcctga
     9181 tgttgttcat cagttgctgc tatggaatat ggttgacagt ctgattaaaa cattaatagc
     9241 cattctaaca atcccactgg ttttctggtt tatgaagaag cagtgccaaa gagttgagac
     9301 aggtaaaatc ggtgatgaag gatactcatg ggagagggga aatcccaaat acaggccgac
     9361 aatggtttgg gatagcaaag gagatattaa ccttcttatc atgccattgg ttggagtttt
     9421 gactctgtgg gggattttta ttattggtgt agtaaccaat atgacttggt taaaaatttg
     9481 gctggcccca aagctttacc ttatcgaata tgcagcatca ttggttaagt aatttcaggc
     9541 cgcatagtcg gcctttattt ttggcataaa caatatgggg taaaaatgaa agttttaatg
     9601 gtttatgaaa atgttccaga gtcgactgaa atctatattt ttgatgccaa tgaagatgaa
     9661 gttaacgatt tgaaattgtc tcacggcaat tacacaaatg ctaattgtga tgaaagtatc
     9721 gaaaaagcac tatcgcgtgt tcttgtcaga attagtgatc cagaacattg tgatgatgat
     9781 tggctttctt attgtggagc ggtaaaaact gatgcaggaa aatggagtaa aagtaaagtt
     9841 gataactcaa ctccaatcat tatgaaagat agtgatattg aaatggtaat aataaccgga
     9901 atgattatgt aggctgagaa taagcactgt gtattcattc caacgagtga atacacggag
     9961 caatgtcgct cgtaaccaaa caggagccga cttgttctga ttattggaaa tcttctttgc
    10021 cctccagtgt gagggccttt ttatatgcat accaataacg cttcacttga ggcgttttcg
    10081 ttatgcaatc aaacagaagg agcatcctat gcaacagttc gctattgcag gggcggcatc
    10141 ggttcgccct ttcaacccga ttttatcggt acagcattca cgaaaaaaca ttttaaccgg
    10201 agcagacttt aaacaacctc gcgttaagag cttgctggat cgtcttgttg agtttctgaa
    10261 tcaaaaggta cagccatgaa aaaaccaact tacgaagaac tggaagaagc cctgaaagaa
    10321 cttagaagaa tggctttcgc ccgacgcact aactctcaca actgcggccc atttcagtac
    10381 tctgatttat gcgaggacat cattgaggta actcaactga ttaaggtcgt taagcaatga
    10441 gcattgcgga tacatggtca gacgaagaat tcattcgtca gatgaacaaa atgctcaatc
    10501 agcacaaaga acaggagaaa gatgatgatt ctgactctga atgataagcg tgaaatatcg
    10561 caaataatcg caagttttac tgatgaagat tacgagcgaa tcaacagtga agttgatcgc
    10621 ctctgcaaac gttgcgaccc aataagcgaa atgcttcgct tatataaacc agatgaacac
    10681 actaaggacg ctatcgactg gctggaagat gatgactgta actatcagga aaaagccgct
    10741 gaatggttct gggatgcaat aaccgaaaga gttaaggctg aatatgcctt cgcaatattc
    10801 aaacgcagac atatttatgg agaagctgca tgagcaatat cgttgaattc gttaaacagc
    10861 aggagcagtt attctgcgga gcattgactg aacagacggt gacatgggct aaggaaagcc
    10921 agtttgcaat tcagtatttc cagaaaaacg attacctggc taaaacagca ctggcaaatc
    10981 caaccagcgc acagaacgcc atcatcaatg ttgcggcgat cggcatcacc ttaaacccgg
    11041 ccagcaaact ggcttatctg gttcctcgcg acggcatggt gtgccttgat atcagttaca
    11101 tgggattact tcatcttgcg caatcgacag gatcaattaa gtgggggcaa tgcaaactgg
    11161 tgtactcaaa cgacacctat gaatcaaatg gccttgattc agcaccaacc cacaaataca
    11221 acgcatttgg tgagcgaggc tctattgttg gaggttattg cacggttaaa acagcagatg
    11281 gtgactacct gactgaagaa atgagtctgg cagaaattaa agctgtggaa gcaacgagca
    11341 aggcaaagaa tggaccgtgg aagacattct gggaagagat ggcgcgtaaa accatagtta
    11401 aacgcgccag caaatactgg cctaaagccc agcgactgga taatgccatt cacctgctta
    11461 acgaagatga aggtatgcat caggaaccag ttatgccgca caaatcagag gaagatatcc
    11521 gcgaagatga acggaaacgc cagcaggaaa taactgataa agcacaactt ctttgtgatg
    11581 aaatggctca ggctgaaaac atggatgatt tgaagagata ttttgcagaa gcatatcgcc
    11641 tgacatctgg aatgaaattg cagcagaaca tacaagccat ttacatagaa tgcaaagcga
    11701 aactggaggt tgccagtgag caaactatat gaaatagcca atgaatacgc aaaattgatg
    11761 gattcagatt tagagccaga gatgattgct gacacaatag aaggcatgga aggagaattt
    11821 accgataaaa tagagcaact tctcgccatt attaaaaatg aatctggtta tgctgaacgc
    11881 ctcaaggaag aggcaaagtc actaaatgaa cgagcagcag taattcaaaa taagattgat
    11941 agcattatgg cgtatatagc gtcatcgctt gaaatggttg gcaagaaaaa gattagagca
    12001 ggtattcacc aggtaacaat ccgcaaaccg tcagaaactg tagaaatcat cgactcaagc
    12061 gccattcctc ctgaatacgt tgagttcgaa acgacaatta aagccgacaa actggcaatc
    12121 aaacaccaac taaaagcagg aataaatatc ccaggtgctc aactcaaagt tgggaaacct
    12181 tcacttctta tcaaataacg gtatcgccta tgaaaaagac tccatgggag aaatgggaag
    12241 tcgatttctt gcgcgaggta gcggcgacaa tgccagttga agttatcgca gaaaaactgg
    12301 aaaggactga aaaagcagta atggcgaaag caacaaggat tggcgctgac attgttagcc
    12361 gacttcgtgg aagacgctgg acaagagccg aagtatcact tttcggtaag ttctccgcag
    12421 aagaaatagc aattgcaacc tgccgctcaa tttattcagt aagagctatg cgatacaagc
    12481 taaaaaaact cgatgaagaa agagcaggca tacgaataaa ttaacaaaga ggaatttacc
    12541 atgagaggac ttgcatacaa tcccggcatt cttccggcag aaatgattat tcgccaacgc
    12601 gtaaagccaa tgccatcgag agaggaattg cttaagagaa attcttttcc atcagtgaat
    12661 caaaacaaat atctgaatgc gatgtggcgg agtgggaaga aatgaaacaa atgtcactaa
    12721 ttgagatgga tggatttctg aaaggtaaat gcatcccacg agatttaaag gttaacgaaa
    12781 caaacgctga atatcttgtc cgtaagttcg gtgaacttga atcaaaactg gaaacggcgt
    12841 tgcgggagtg tcgttctgct ggaatcacga ttgataacct tgaggccaag tgcgcggcgc
    12901 tggcggcgga gaatgcggga ataaagtctg caattccaga atcacgggat attgaggatg
    12961 acaatgacaa tatggatgac gtatctctcg cggaagactt cgggttcaat catgcaatag
    13021 aacggatgag gagacagata cctgaaacgc caaccactga tgctttcctg gctgaagtcc
    13081 gggcgcaggg ggtggatgct gctatagaag ctgcaaaaaa tctggtggcc caagaatatg
    13141 agtataagga tttcaaagcg gcgcagagtg attgctgtat gcaccctggt tcagacctgg
    13201 tagggaaggt tgaaatgact gagtggttag ttgactttgc tgcccagctt cgcaaaggag
    13261 gcagccagtg agcaagattg attatcaggc actgcgtgag ttagcaaaac aggcaacaca
    13321 aggagaatgg gttgcattta tttcgacggg tactggtacg tatgcggtgc atacgcccgg
    13381 tgataaacga tgtgaagacg ttatcaaatg gaccggcttt gatggacaga aaaacgcaga
    13441 gaacaacgct cgttatatcg cagctttcaa ccctgaagta gtacaggcac tgctggatga
    13501 gaacgaaagc atgtgccgcg acctcattgc tcgcaatggt gagattgaag ggcttcgaaa
    13561 actggttgct gagcttgaag agacaaaatc aaaactcaac gagcagcgtg aatattatga
    13621 aggtgttatc tcggatggga gtaagcgtat tgctgaactg gagaaaagcg aagagcagct
    13681 cattaacgag cgtgaccatg ctgagtctgc tttagctgat atgtattttg cagcaaccgg
    13741 ggacaggccg gaatggagta actgtttcag tttttcagat gcagtagatg ccgtggttga
    13801 cagaattgct gatttagaag ctaaacaggc atcgccagta gtaccggaag aactgattaa
    13861 agcagtgcgc ttttatgaac aggtaaagcg tgagaatcca tctgttgaaa ccggagcatg
    13921 gaaagacgct gttgactggg tactcaaaga ggcttgccag gctgtaagca ttagcaacaa
    13981 agggggtgag taatgcgtgt ggcatgtatc agtttgttac cgtacccgac tcgtttttgg
    14041 gcttctgcgc taattgcaaa gccgcgtgtc ctgatggctg acaacatcat cccggcacca
    14101 aagcgccgcc atacaggtat tgcggcggcg agacgggcag caaagaaacg caggagagca
    14161 aaacgatgaa aaaccgtaaa gcaaaaattc tgttagctcg cagaaacggt gttggggtct
    14221 ggcgatggtt gaggattagt aacagacgag tgaggttgac ggggtgttgc ggggtgatgg
    14281 ggcacagttg ttgcaaaaag cccagtgcag cgcaaaaccg ctggaaaaac cacttacgca
    14341 ctaaaggagc ctgatatggc tacttttacc gacaaagaac taattaaaga aatcaaagag
    14401 cgcataggca gcctggacgt tcgagacaat attgagcgcc gtgcttatga aattgcactg
    14461 gcatcactgg cagcagtatc ggatgaacga gcagcctatg aattatttat ggagaagcgt
    14521 ttcggggaat ctgtagatcg ccgtagggca aaaaatggcg atagagaata catggcatgg
    14581 gatatggcgc ttggctggat tatctggtgt caccgcgccg ccatgcttca ggccggaaac
    14641 tttcgggaaa ataagaattc gtcaaccaac aattttcggg aaatctcgga aacgtcaacc
    14701 aactctccgg taattccgga tggttagata agctgtagtg agcgaatgcc tgaaaagggc
    14761 cagaacgtgc ttatttcggt gaatttcgat agctctctgg ttgaaccgct aatatgctcc
    14821 gcacgctata ccggaagcac ctttcggcgt gggcagataa cggttgcgcc tggtaatggt
    14881 attgatggta atggtattga acaggccaca cactggatgc cgctaccgga accgccgcag
    14941 gaggttaacc gtggctaacc tgcaacttgc cgtcaaaggt gaatacttcg atgccatgat
    15001 tcgcggggag aaaacggaag agtatcgcct gtgtaatgac tactggaata agcgaattat
    15061 gttccgggaa tatgaccgtc tgattatcac aaagggatat ccgaagcgcg acgattccag
    15121 ccgcagaatt gacgtcccgt atgaagggta tgaaatcaag acaatcacac atacacactt
    15181 cggagataaa ccggtaaagg tgttcgcgat aaaggtgaat atcggcagtg attgacttcc
    15241 tcgcactcgc ggggatttct tttatctgaa aaggacagaa tacatgatta acaatattgc
    15301 aaatcagtct tcgtttccgg ctgcaacaat cgacagccag atgctgttga aaatggtcaa
    15361 tgaggcacgg aaattatgta gtgaaaagcc cgttcgcaat aatgatttca ttgccagagt
    15421 taaggatgag cttgaaggag agacctacga aattttcgta ggtcagaaaa acggcgcaga
    15481 aatagaagtt ataacaatga ctttcaagca agccctgcga gtagccgcgc gtgagtcgaa
    15541 ggccgttcgt cgttcgctgg tcgataaact ggaatcaatg caacagcagt tgcaacaaaa
    15601 aaccaccacg aagaaatcac cggatggcct tgaagaattc cgtaaggcac gcgcattgaa
    15661 aatgaccgtc gacacaatga aggacctctt cgacttcctc cctaaccttg caccggaagc
    15721 taaacaggtc gttgccgcca gcctggttaa tcccgttgta ggcgctaacg taatcccgct
    15781 accgatgatc aatgaacatt actattcggc gtcagaagtt ggagcaaaac tcaaaatatc
    15841 cgctaacaag gttggtcgca ttgccaatac ctacatgctc aaaacagaac aatacgggaa
    15901 atggttcatc gacaaatccc cgtattcgga taagcaggta gaaagctttc gatacaataa
    15961 tcggggagta cgaaagattg aagaaatcct ggacgcggag aacaaagcag agttcggaac
    16021 ctgatatcct ccactctccg acgccgccag aatgacggcg cggtggttaa gcatgaggcg
    16081 aaatttctgc cgggcggtat tttgttgaga gatgagatac gtcaacttct ctttccgctt
    16141 tttcaaggct ccatgccgtc tggagacgaa tccatgaaga cggagttcca cccaggacag
    16201 cagcgagacg aacagccatt tcaggagata ttgtcgtatt acctgaaacc agtcgctgaa
    16261 cagtggaagg cgcgacatca agcgctcttg ccagttccct gattccaata ttctgatctt
    16321 ccagaatatc agcaataatt tctccgggat gaggggggtt aaacatcgcc attagtggta
    16381 atcctcgtag ttaaacgaag cggctctgga gttttttccc tgtagcacgg gcgaattttt
    16441 tcaacgttgc aaatgatggc cttatttaca ctcaatggtt aattctttcc tgttaaatcc
    16501 ctgttccttt aaaatgtctt tatatccatt tttacatagg gcattggctt tatcatagca
    16561 tagtccccat ggtgtagcgg caccacaggc aatgatgtac tgttcctttt catctccgcg
    16621 atttattttc gtagtggtag tgcatcctga tgttgccatg cagaaaatta tcaaataaaa
    16681 gaaccttgct atgatgttca ttatcggatc tctaataggt gctgcaagtg acactattcc
    16741 catacctgga gcagtttgtt agtgtggcac ttcatatgcc atgcagatta cttccgggcg
    16801 cgaatctcag agcgaacctg tgaaatggag tgccctttgc ctaggttctc acgaatagcg
    16861 tcgagtggcg gcgcggctga gtgtattaac cacgcttcca ttgctttgtc gcgttcattc
    16921 agtgcacgaa gcccttcacg aataacttcg ctttctgaag cgtatgcacc ggaagccaca
    16981 cgggcgcgca ccatgtcagc catttcgttg gttaatgtaa tgctgaattg ttgggttgta
    17041 cgcatgttaa acctcactga gtaggataga acactattcg atgatagcac gccacttgtt
    17101 gacgacaaca catatccggg attatattct tcgcacggtc gagttgaccg tcgggattcg
    17161 aaccccggat agaaaccgcg acagagacac gccgcgagcg tgttttttat tgtcgtatgc
    17221 acgcgcacat ctgaattatg gtggggcgta taggggagct gaaaagctcg ccggttggtt
    17281 tcccggtagt tcgaaccctg tacgtctcac cacccgatga ttcgaaccta acggtggtga
    17341 tagtttagaa accactcgag ggcgtcataa tgacaactca agtttctgtt gaaacactct
    17401 ccacgattac ttacaagcag atccccgtta tcactaccga acttttggcg cacctttacg
    17461 gcacagaagc tattcgtatt cgccagaatc accacgaaaa caaaggtcgt ttcattgagg
    17521 aaaaacactt cttcaaactt gaaggtgaaa ctttacgtga gttcaagcac agagtagctt
    17581 ttaactactc tgtgaaaatc gcccgtaacg ttcgctccct catcctctgg acagaacgcg
    17641 gcgcagcccg tcacgccaaa atgctcgaaa ccgatcgggc gtgggaagtg ttcgaaaaac
    17701 tggaagactg ctatttcagt caaaaaatcc cagaacaact tcagcttcca gaatcaacgc
    17761 tatctatcaa ctaccctctc tcgtggtttt cagagcacca cccctactcc atgatgagct
    17821 atgttgatcg aaaaactctt aacctggacg tttctgtact cttcgacatg ccaagcccaa
    17881 ctatgcgcat cctcaacgag ctacacagca aaggctataa cgttgacgcc gctgtcgccg
    17941 aatttaacgc cttcaagcat ctgacggaag aaatgcgccg tactctacag gatatttcaa
    18001 gattgtcaga tcgaaattcc cgaaaaggct tctcgttaag cctgtaactt cccctaccat
    18061 ccccgacatc ccgtcggggt tttcatatct ggagacaaaa atttgaaaca gatcgctttc
    18121 tacaggcgtt ctggaaggcc cggtgccttc cgtgggttaa aagaacgcgt cacctggatg
    18181 attcagtctc gtggtcgtcc tgttactggc agtgaaatag cagagaaatt tggtgtttca
    18241 ctttgcgagt tcaacaaggt agctcgcggc ctgacaaagg gcagtaaggt tgtgaaaata
    18301 aaggcttcag aaccattcac cacagacacc ggaatcgttg atcgcttttt ctccctcgaa
    18361 tcaaatcctc gtcgtgacac acctcgctca cgcaatgccg ttcctccatt cagtcgcaga
    18421 agccgtgaac acgcagcaaa aaactgtcgc gaggaatacg tgcaaaaggc cgaacgccgt
    18481 cgccgactga ttaaagcagg actttacatt gatgagtttg aaaacgcgct atgacgaaaa
    18541 aatacaccct catttacgca gatccaccat ggacattccg cgacaaagca accgatggtc
    18601 aacgcggtgc aagttttaaa tatccggtca tgagtcttct ggatatctgc cgcctcccgg
    18661 tatgggaact ggcagccgat aattgcctgt tggctatgtg gtgggtgcct acacaaccac
    18721 ttgaagcatt gaaggttgta gaagcgtggg gctttcgtct ggtgacgatg aaaggattaa
    18781 cctggaacaa atgcgggaaa agacagaccg acaagctggt catgggtatg ggtagcacca
    18841 ctcgcgctaa cagcgaagac tgcctttttg cagtgaaagg aaatctgccc gaacgcatta
    18901 acgccggaat aatccagtca ttcactgcac ctcgccttga tcactcccgc aagccggata
    18961 tggctcgaga aaaacttgtg caacttcttg gcgatgttcc ccggatagaa ctgttcgccc
    19021 gccacacctc gcatggattt gatgtatggg gtaaccaatg cggcacacca tccattgaga
    19081 tggttccggg tattgttaaa tttctggaga aaaccaatga gcgaaaaaac gacgttgaca
    19141 aaggcatcac cagttgaatt aaggcagtgt ctggaaatcg caaatcaact tgccagaaga
    19201 ggaatacgat ttgttccaat cccgattaca gcagatgcag aacttcatct gtttggtgaa
    19261 attctttccc gaaagctgga tgaactggaa aagctggtag aagaagctga cacctcacca
    19321 accgtataac agccccaccg acattaaaat atcaggagaa aaaatgaacg cagtgctcac
    19381 agaattgaac aaattaggaa aagcatcagc cgaaagtatt tctaaaggtc tcaatattga
    19441 tttgaatgac gttattgaca ctctatggaa gttaaaaaac cagggggtag taactgtaaa
    19501 aaatggcatc tggcaggcag ttgcaaggga agtggacaaa aaaccaaata tcgcctcagt
    19561 gcagccagtg cagccagtgc agccagtgca gccagtgcag ccagtgcagc cagtgcagca
    19621 caacattata ggtgacctgc tacgtaaatc acggaaagaa gcgcgtcgcg ccgggcggaa
    19681 acagaaacga tgggagggtg catgtaaggc gctgcaagaa ctgaataaat accgtgactt
    19741 gatcaacgaa ttgtcagagt gagcgaatca cagagagaat attttgagaa acaacccgcg
    19801 tacattaata ttgtttttcc tttctctaat gacgggcggt atcctgttat tcgcaggaac
    19861 aacattattt atttttgttg cccggctaac tgcgaggata atgacatgaa aatcacattt
    19921 gaaagttacg gtttaacagc aagtgtggtt atctccagct ctatattcga aagccgcaaa
    19981 catcagcata tcgttgatgc agtgaaactc aaagccccgg aagttaccgt cacaacacat
    20041 ggacttttca ggatacgaac gacgttgact acaacacacc tctcggcatg ccgtgtttat
    20101 gatatggcac tcaaggaata caaccagtgt tcgtcctgat ccagcgcggg cagtctttcg
    20161 ttgatgccaa caactatccg gtagaaatat gcaaggtaac tctgactcag gtgatctacc
    20221 gaaggctcga cggcagaacc agagccactt caattggtgc atttaatgaa gaatttgagc
    20281 gaatagagca caacgaactg catatgatta aagcggaaat tgagaaggaa aagcatattg
    20341 ccagccttcg aaaaatgcgc cgtacatcaa tcaactaaca accgccttcg ggcggtttat
    20401 tatgctgttg aaaaacatgt agaaaaggag agcatactat ggaaatcaac ttaatgaccg
    20461 aaaaagaagt ttctgagttg ttacaaaaaa ggcgcacagc tttgtataat ttgcgaaaaa
    20521 aacacggatt ccctgatcca gtacttactc atccggcgcg atatagccgt caggccgttg
    20581 aacaatggct taaatccgga ggaattaacc gagctgttta gcgtgccaga aaattttatc
    20641 agcatacaac tcatacgcat ctttctgttc cgacaaccag tcgtgtttat tatacacggc
    20701 catcactcct cccagttcat gccccagcat tttttcagtg acgtggggca taattccttc
    20761 cccagataaa ttcgttacca gagaacgcct aaaatcatgc gttctccatt caggaatatc
    20821 aattttcgcc ctgagttttt tcatgtacaa attagcagac gaacggtcaa taggtttatc
    20881 tatttcctgc ccagggaaca gcacatcaaa tccgatatta agcaatcgct caatataagg
    20941 ctcaacctga gtaaatatcg gacgccggat aatattaccc atctttgaat gctctttagg
    21001 ggttgtccag accagatctt ccatgttgaa ctcactggct gtagcgagac gtagctcaga
    21061 aagcctggcc ccccatagca atagcaattg atgtaacagc ttgttggaag taaccacttt
    21121 tgagttctcc agtgtcagcc agatctttgc cagttccgta taggtaagta cgcgactccc
    21181 cacatctggt ttcttcccaa tattctttac actaagtttg agtaattcgc atgatgggat
    21241 taactggcgg ctgatacacc agttaataac tgaacgcagt tgtaacaaca gaacacgagc
    21301 ctttttcttg ttgaggcttt cttgcttgtc aaaaaagcga gcccaagccg atacagggat
    21361 accggcaact ggcgagtctt caaactgtgt gtacatagtg ttgtacacaa cagatctata
    21421 aagcgtctga gtattgggtt tcaggccgct aacatactta tcccaccact gatccaggca
    21481 ttcttttaat gtcaattcgc catcactggg ggcaaaataa tttttagggt taactccctt
    21541 catgtacaat gcgcgcatct caccgacgat aacacgtgcg tccttcagtg aagtggacgg
    21601 atagcgcccg acagtaagac gtactggctt accattccag cgataacggt actgaaacgt
    21661 gatagttcct gctggagtaa tccgcacact taacccgtca ccatcggtga tttcagcagg
    21721 gccagagtag ggttttccgt ataggcctcg tagtttggtg tcactcagtg ccaatgcttt
    21781 atatcctgta cacaagttta taatgtattc tgtactcaat atgtacgcat tagcaagtga
    21841 acaaaacact tttcaacgca aaagaagata atcaaccatg aacaaaaacg aacttacacc
    21901 ttgattttaa aaccataaag acggtattat cagtcatatg gtgaacaacc aaaatcaata
    21961 cgcaacaacg tcctcttagt taaatggata taacgagccc ctcctaaggg ctaattgcag
    22021 gttcgattcc tgcaggggac accagatacc cttcaaacca aatctacctt caccccgtaa
    22081 aagatgggtt tggcagcaca cttgccctat atctactcat ttttactgca acaggttgaa
    22141 atctcagcac tgtcagaaag cgctgatgac taaacagccc tgggccgggc gatgtaacca
    22201 tcatacagaa tcctgatagc gaaatatggc gtgactcgat acttcactct gcaatgcatt
    22261 ccttgatgaa ttcgcaggcc cgtgatacac gggacacgtc gctgaatgac gacaatgtcc
    22321 tggaaatcag cgaaccgcgt atccggagta catttgagcg actgtaccag aacatgaatg
    22381 aggcgtttgg attaggtgat tattagcagg ggtaagcatt tttgtattat tattttccgg
    22441 ttgagggata tggagatatc gacaacaacc ggaaaaagtt tacgtctata ttgctgaagg
    22501 tacaggcgtt tccataacta tttgctcgcg ttttttactc aggaagaaaa tgccaaatag
    22561 caacatcagg cagacaatac ccgaaattgc gaagaaaact gtctggtagc ctgcgtggtc
    22621 aaagagtatc ccagtcggcg ttgaaagcag cacaatccca agcgaactgg caatttgaaa
    22681 accaatcaga aagatcgtcg acgacaggcg cttatcaaag tttgccacgc tgtatttgaa
    22741 gacggatatg acacaaagtg gaacctcaat ggcatgtaac agcttcacta atgaaataat
    22801 ccaggggtta acgaacagcg cgcaggaaag gatacgcaac gccataatca caacaccgat
    22861 aagtaatgca ttttttggcc ctacccgatt cacaaagaaa ggaataatcg ccatgcacag
    22921 cgcttcgagt accacctgga atgagttgag ataaccatac aggcgcgttc ctacatcgtg
    22981 tgattcgaat aaacctgcat aaaagacagg aaagagttgt tgatcaaaaa tgttatagaa
    23041 agaccacgtc cccacaataa atatgacaaa aacccagaag tttcgatcct tgaaaactgc
    23101 gataaaatcc tcttttttta cccctcccgc atccgccgct atgcactggt gatccttatc
    23161 tttaaaacgc atgttgatca tcataaatac agcgccaaat agcgagacca accagaagtt
    23221 gatatgggga ctgatactaa aaaatatacc agcaaagaac gcgccaatag catagccaaa
    23281 agatccccag gcgcgcgctg ttccatattc gaaatgaaaa tttcgcgcca ttttttcggt
    23341 gaagctgtca agcaaaccgc atcccgccag ataccccagg ccaaaaaaga gcgcccccag
    23401 aattagacct acagaaaaat tgctttgcag taacggttca taaacgtaaa tcataaacgg
    23461 tccggtcaag accagaatga aactcataca ccagatgagc ggtttcttca gaccgagttt
    23521 atcctgaacg atgccgtaga acatcataaa tagaatgctg gtaaactggt tgaccgaata
    23581 aagtgtacct aattccgtcc ctgttaatcc tagatgtcct ttcagccaaa tagcgtataa
    23641 cgaccaccac agcgaccagg aaataaaaaa gagaaatgag taactggatg caaaacgata
    23701 gtacgcattt ctgaatggaa tattcagtgc cataattacc tgcctgtcgt taaaaaattc
    23761 atgtcctatt tagagataag agcggcctcg ccgtttactt ctcactattc cagttcttgt
    23821 cgacatggca gcgctgtcat cgcccctttc gccgttactg caagcgctcc gcaacgttga
    23881 gcgagatcga taattcgtcg catttctctt tcatctgtag ataatcccgt agaggacaga
    23941 cctgtgagta acccggcaac gaacgcatct cccgcccccg tgctatcgac acaattcaca
    24001 gacattccag caaaatggtg aacttgtcct cgataacaga ccaccacccc ttctgcacct
    24061 ttagtcacca acagcatggc gatctcatac tcttttgcca gggcgcatat atcccaatcg
    24121 ttctgtgttt ttccactgat aagtcgccat tcttcttccg agagcttgac gacatccgcc
    24181 agttgtagcg cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca tagatcttca
    24241 cgaatattgg gatcgaagct gacaaaacct ccggcatgcc ggatcgccgt catcgcagta
    24301 aatgcgctgg tacgcgaagg ctcggcagac aacgcaattg aacagagatg taaccattcg
    24361 ccatgtcgcc agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact ggggcggacc
    24421 ataaacgtaa atgaacgttc tccttgatcg ttcagatcga caagcaccgt ggatgtccgg
    24481 tgccattcat cttgcttcag atacgtgata tcgacaccct cagttagcag cgttctttgc
    24541 attaacgcac caaaaggatc atcaccgacc cgacctataa acccacttgt tccgcctaat
    24601 ctggcgattc ccaccgcaac gttagctggc gcgccgccag gacaaggtag tagccgcccg
    24661 tctgattctg gcaagagatc tacgaccgca tcccctaaaa cccatacttt ggctgacatt
    24721 tttttccctt aaattcatct gacttacgca tagtgataaa cctctttttc gcaaaatcgt
    24781 catggattta ctaaaacatg catattcgat cacaaaacgt catagttaac gttaacattt
    24841 gtgatattca tcgcatttat gaaagtaagg gactttattt ttataaaagt taacgttaac
    24901 aattcaccca atttgcttaa ccaggatgat aaaaatgacg caatctcgat tacatgcggc
    24961 gcaaaacgca ctagcaaaac ttcacgagcg ccgaggtaac actttctatc cccattttca
    25021 cctcgcgcct cccgccgggt ggatgaacga tccaaacggc ctaatctggt ttaacgatcg
    25081 ttatcacgcg ttttatcaac atcacccgat gagcgaacac tgggggccaa tgcactgggg
    25141 acatgccacc agcgacgata tgatccactg gcagcatgag cctattgcgc tagcgccagg
    25201 agacgagaat gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct
    25261 ctcacttatc tacaccggac acgtctggct cgatggtgca ggtaatgacg atgcaattcg
    25321 cgaagtacaa tgtctggcta ccagtcggga tggtattcat ttcgagaaac agggtgtgat
    25381 cctcactcca ccagaaggca tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga
    25441 cacatggtgg atggtagtcg gggcgaaaga tccaggcaac acggggcaga tcctgcttta
    25501 tcgcggcagt tcattgcgtg aatggacttt cgatcgcgta ctggcccacg ctgatgcggg
    25561 tgaaagctat atgtgggaat gtccggactt tttcagcctt ggcgatcagc attatctgat
    25621 gttttccccg cagggaatga atgccgaggg atacagttac cgaaatcgct ttcaaagtgg
    25681 cgtaataccc ggaatgtggt cgccaggacg actttttgca caatccgggc attttactga
    25741 acttgatagc gggcatgact tttatgcacc acaaagcttt ttagcgaagg atggtcggcg
    25801 tattgttatc ggctggatgg atatgtggga atcgccaatg ccctcaaaac gtgaaggctg
    25861 ggcaggctgc atgacgctgg cgcgcgagct atcagagagc aatggcaaac ttctacaacg
    25921 cccggtacac gaagctgagt cgttacgcca gcagcatcaa tctatctctc cccgcacaat
    25981 cagcaataaa tatgttttgc aggaaaacgc gcaagcagtt gagattcagc tgcagtgggc
    26041 gttgaagaac agtgatgccg aacattacgg attacagctc ggcactggaa tgcggctgta
    26101 tattgataac caatctgagc gacttgtttt gtggcggtat tacccacacg agaatttaga
    26161 tggctaccgt agtattcccc tcccgcaggg tgacatgctc gccctaagga tatttatcga
    26221 tacatcatcc gtggaagtat ttattaacga cggggaggcg gtgatgagta gccgaatata
    26281 tccgcagcca gaagaacggg aactgtcgct ctatgcctcc cacggagtgg ctgtgctgca
    26341 acatggagca ctctggcaac tgggttaaca taatatcagg tggaacaacg gatcaacagc
    26401 gggcaaggga tccgcgtcac tcttccccct tcacgatctt caataatatg caatgcagct
    26461 tcccgcccga taatgtcatg tggaagctga attgtggtca gcggcggtaa aaacagatgc
    26521 ccgacgccaa ccagattatc aaagcccatt acggcgacat cctgcgggat acgtaccccc
    26581 ttcgccagga gaacctgata agcgacaaag gctgcgcgat cgttaccaca tatcagaaca
    26641 tcaaaatctg gtttgcccga tttgaagtgg tcattgagta aacttgcgag atcggtgtag
    26701 tgatcatcac ctgttgccat gtgaaattgt ttcacctcag ccagatctcg tccagcatca
    26761 cgccaggcct gctcaaatcc ctgccgacga taccctgttg ccaacgcact ttccggtagc
    26821 cagaagcata acggttgacg atagcccgcc gcgagcaaat gctgtgttga ttcatattgt
    26881 gcagtgtaat catcagggat ataactgggt aacgctgggt catccgccac acagttcgcc
    26941 aatacaatat tttcaccata cagagactca ggcagcgaga tatgtcgcag ccccattgta
    27001 gtatagataa tgccatccgg acggtgggca agcagctgac gtgccgcgcg ggcagcgtca
    27061 tcttcagaaa aaatattgat taaaaaacta ttccagccaa actcgctggc ggtttgctca
    27121 atggcaagca gaatatcaac agagaaagga gtggtggccg tgtcctgcgc cagcacggcg
    27181 agagtcgacg gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc aggaacataa
    27241 ttcagggtct ggattgcctg caatacgcgg tcacgcgttg caggacgcac agattctgca
    27301 ttatgcatca cccgggagac tgtcatcatc gacactcccg ccaggcgtgc gacatccttt
    27361 aatgaagcca tacccaagcc gtttgccgta aaacgggcac tgtagcagaa acagacgtca
    27421 ctggcgagat ccaacgatat tctcaacgcc ctatcacccg acacggcaat acaataaaaa
    27481 ataacaataa ttcccggaca attgtaccca attccgcctc tgttctcgcc aacgagtaac
    27541 catgtaggca agtaggtaaa acatgccaac tggcatcgcc aacctggaga agaaaatata
    27601 aatatggatc attgtgctgc cgctgtatcc cgacatcagt ccggaaatta ttgcgattgc
    27661 tatcggttca ggtgcgattg gctgcacgat cgttacggac tcgcttttct ggctggtgaa
    27721 gcaatattgc ggcgctacgc tcaatgaaac atttaaatac tatacgacag cgacatttat
    27781 cgcttcagtc gtcgctctgg cgggcacatt cctgctgtca tttatcatct aagcgcaaag
    27841 agacgtacta tggaaaacgc taaaatgaac tcgctcatcg cccagtatcc gttggtaaag
    27901 gatctggttg ctcttaaaga aaccacctgg tttaatcctg gcacgacctc attggctgaa
    27961 ggtttacctt atgttggtct gaccgaacag gatgttcagg acgcccatgc gcgcttatcc
    28021 cgttttgcac cctatctggc aaaagcattt cctgaaactg ctgctactgg ggggattatt
    28081 gaatcagaac tggttgccat tccggctatg caaaaacggc tggaaaagga atatcaacaa
    28141 ccgatcagcg ggcaactgct actgaaaaaa gatagccatt tgcccatttc cggctccata
    28201 aaagcacgcg gcgggattta tgaagtcctg gcacatgcag aaaaactggc tctggaagcg
    28261 gggttgctga cgcttgaaga tgactacagc aaactgcttt ctccggagtt taaacagttc
    28321 tttagtcaat acagcattgc tgtgggctca accggaaatc tggggttatc aatcggcatt
    28381 atgagcgccc gcattggctt taaggtgaca gtacatatgt ctgctgatgc ccgggcatgg
    28441 aaaaaagcga aactgcgcag ccatggcgtt acggtcgtgg aatatgagca agattatggt
    28501 gttgcagtcg aggaaggacg taaagcagcg caatctgacc cgaactgttt ctttattgat
    28561 gacgagaatt cccgcacgtt gttccttggg tattccgtcg ctggccagcg tcttaaagcg
    28621 caatttgccg agcaaggtcg tattgtcgat gctgataacc ctctgtttgt ctatctgccg
    28681 tgtggtgttg gcggtggtcc tggtggcgtc gcattcgggc ttaaactggc atttggcgat
    28741 catgttcact gcttttttgc cgaaccaacg cactcccctt gtatgctgtt aggcgtccat
    28801 acaggattac acgatcagat ttctgttcag gatattggta tcgataacct taccgcagcg
    28861 gatggccttg cagttggtcg cgcatcaggc tttgtcgggc gggcaatgga gcgtttactg
    28921 gatggcttct atacccttag cgatcaaacc atgtatgaca tgcttggctg gctgacgcag
    28981 gaagaaggta ttcgtcttga accttcggca ctggcgggta tggccggacc tcagcgcgtg
    29041 tgtgcatcag taagttacca acagatgcac ggtttcagcg cagaacaact gcataatgcc
    29101 actcatctgg tgtgggcgac gggaggtgga atggtgccgg aagaagagat ggagcaatat
    29161 ctggcaaaag gccgttcata acgtttcaac gcagcatcgc catcctttcc ctgggtgagc
    29221 gatgctgccg atggcgcaga cttaagatcc ccggtcttac ctgctataat tctctcttta
    29281 taccatcacc caacgccttt cgctgtaaac ggcggtttcg caaaccaaac caataccgta
    29341 agtaagataa aagcaatagc cgccatacgg aaaatctcat ttgcagaaat tgaaagtgac
    29401 tgctgagtta tttcattatt aatttcatta agaactcctg aaagcgaacc atagtattta
    29461 tccataattt gtgacgatga attaaatacg gggttaaatt gatcgatggt tgctgtcaac
    29521 tgactatggt gtaacgattc gcgtcgtccc cacagcgtca ttgtcaacga cgtaccaact
    29581 gatcctgaca aggtacgaaa aaaattactc atactcgagg cattggcaaa tttattatct
    29641 ggcaaccctg aaaacgaaat cgttgttaag ggtaaaaaga aacaggcaac ggcgaatccc
    29701 tggaaaaact gtggcaaaat gatgcctgta aaatcaatcg ttggcataaa tgtcacagaa
    29761 cgccagtaat agcaaaccgc atacatcaaa aaactaaatg tcaccaacaa ccgcatatca
    29821 attttgttgc cataacgtcc tatcaaaggt gatattaata gcggcatgat gccgatgggc
    29881 gcataggcaa gtccggccca tatcgcatta taccccatcg tttcctggag taactgcggc
    29941 ataaggacta tcgccccaga gtaaaataaa tacgcgcatg tgatactcac aataccaatg
    30001 gtgaaattac gggacttaaa caaactgaga tcaagaatcg gattctctga ggtcgactcc
    30061 caaatgacta aagaaatcag agaaataact gacactactg ttagtattat tattgtactc
    30121 gagttgaacc aatccagatc gcgcccttta tcaagcatta tctgcaagcc accaacaccg
    30181 agcactaaca gggtcagtcc tggtagattc attttgaccg gtgaagtctc agtttctctt
    30241 cctttaagta aggttaagca taatgtcaga acgataatcc ccatagggac attgattaaa
    30301 aatatccaac cccagctaaa gttatcgcaa atatagccgc ccaatatcgg cccacatatc
    30361 ggagcgataa ttacggtcat tgaccataat gccaaagcaa atgttctttt ttctggcgga
    30421 taattcctta ataataaact ctgtgacagt ggaattaacg gccccgccat taagccctga
    30481 acgactctaa aaaatatcag cacatcaaga ttggtcgata ggctacacat taatgaagat
    30541 agtgaaaaaa aggttactga aagtaaaaat aatcttaatt cgcctattct ttgtgccagc
    30601 ctgccggtaa cagggatcgc aatggcattt gctacgccaa acgaagtgat aacccaggtg
    30661 ccttcgtctg ttgatgctcc cagaaagcca gatattgtcg gtattgcgac gttagaaata
    30721 gtggaatcca acatttgcat aaacgtcgct aaggacaatg caatagtaac gcaccataac
    30781 gtcccaccgg ttaatggtgc cggagttgat ttagtgattg ccattaaagt tgtccattat
    30841 gcgaaataat gttgctaatt tctttttcta tcggagtagt atcgataact aaagccttac
    30901 tggtataagc aggcatggag gtcacggttg aagccagctc aggcatctca gcaatgtctt
    30961 cgtccttcgt atcaatagtt gcagtcatcg ataaaccaat acgtaaggga tgttccatga
    31021 gttcttttgg atcaagagaa acttcaaccg gtacacgctg aacgattttg atccagttcc
    31081 ctgtcgcatt ttgtgcaggt aataaggaga acgcattgcc ggttcccata ttgattcctg
    31141 tcacccgacc atgaaacaca acattttcac cataaagatc gctgataata ttgaccgatt
    31201 gaccaatccg tacatccgtg agttgtgttt ctttaaagtt ggcattaacc cacatttgac
    31261 gtgccggtac gacagccatt aacgattgtc cgggactcac tgtttcgccg acctgaacac
    31321 ttcgctgggc aatatagccg gtaacaggac tcttaatatc cgtacgttta agcgctaacc
    31381 aggcttcttt ggttgcatct gccgcttcaa cgacttgtgg ctgacggttt aatggtgtgt
    31441 tcattactaa agctttattc gctttataag cctggatagc ggcattcaat gccgctttgc
    31501 tacttattaa cgtatctttg gtatgctcca gcgtttcttt tgaaataacc ccctgcttcg
    31561 ctaaaggcac acgacggtta taatcttcta aagattgttg atactgaata cgtgctgaag
    31621 cgacttcggc actgtattgt ttatcctgta agtacagttt attcgtttgc cgaacaatat
    31681 ttgccagatt atttttagct ttattgagtg cgatagtggc atcagtctta tccagtgaga
    31741 ctaaaatgtc accctgtcga acgtagttcg tatctttatg attaacgaca gtgacgctgc
    31801 ctgaaacttg tgcagaaatt ggatctgcat tccccgtgac ataggcgtca tctgtactaa
    31861 tcatgtcttc taattccatt gaccaatagg cataggcacc tgaaaacgca ataaataaaa
    31921 ctaccgccaa taaagaaaag tattttcttc tgttagaatg ttttttattt gaattaatct
    31981 gttccacttt tatctctcat ttcgcataga tgaaatttat gaattgaatg aaaagagtat
    32041 tcattcttca tatcaaacac agtgcatgtg ttgcacatta tcccaggaac gttggaggcc
    32101 taatttaaat gaggtgcaaa aaaaacaaca gaggactaaa ccgtggcttt tgcaatacaa
    32161 ttcttacgca tgtaggatta gtgagaagac ttatagtgc
//
LOCUS       DAZKJR010000046        32180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKJR010000046 DAZKJR010000000
VERSION     DAZKJR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.046"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(39..1658)
                     /locus_tag="ACHOWS_002157"
     CDS             complement(39..1658)
                     /locus_tag="ACHOWS_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0047723.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLANVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTPKKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSDVVIDYLKKGHKITPAQVNEMRVDVSK"
     gene            complement(1699..3249)
                     /locus_tag="ACHOWS_002158"
     CDS             complement(1699..3249)
                     /locus_tag="ACHOWS_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0047724.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANISGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGIRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            complement(3482..4606)
                     /locus_tag="ACHOWS_002159"
     CDS             complement(3482..4606)
                     /locus_tag="ACHOWS_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFQ0047725.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            4739..6292
                     /locus_tag="ACHOWS_002160"
     CDS             4739..6292
                     /locus_tag="ACHOWS_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0047726.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            6674..7564
                     /gene="metF"
                     /locus_tag="ACHOWS_002161"
     CDS             6674..7564
                     /gene="metF"
                     /locus_tag="ACHOWS_002161"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFQ0047727.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7893..10073
                     /gene="katG"
                     /locus_tag="ACHOWS_002162"
     CDS             7893..10073
                     /gene="katG"
                     /locus_tag="ACHOWS_002162"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFQ0047728.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10167..11072
                     /gene="yijE"
                     /locus_tag="ACHOWS_002163"
     CDS             10167..11072
                     /gene="yijE"
                     /locus_tag="ACHOWS_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFQ0047729.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(11099..11716)
                     /gene="yijF"
                     /locus_tag="ACHOWS_002164"
     CDS             complement(11099..11716)
                     /gene="yijF"
                     /locus_tag="ACHOWS_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFQ0047730.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(11992..13095)
                     /gene="gldA"
                     /locus_tag="ACHOWS_002165"
     CDS             complement(11992..13095)
                     /gene="gldA"
                     /locus_tag="ACHOWS_002165"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFQ0047731.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13106..13768)
                     /gene="fsa"
                     /locus_tag="ACHOWS_002166"
     CDS             complement(13106..13768)
                     /gene="fsa"
                     /locus_tag="ACHOWS_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0047732.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13780..16281)
                     /gene="ptsP"
                     /locus_tag="ACHOWS_002167"
     CDS             complement(13780..16281)
                     /gene="ptsP"
                     /locus_tag="ACHOWS_002167"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0047733.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEIKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16590..17669
                     /gene="frwC"
                     /locus_tag="ACHOWS_002168"
     CDS             16590..17669
                     /gene="frwC"
                     /locus_tag="ACHOWS_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFQ0047734.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17684..18004
                     /gene="frwB"
                     /locus_tag="ACHOWS_002169"
     CDS             17684..18004
                     /gene="frwB"
                     /locus_tag="ACHOWS_002169"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFQ0047735.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            18055..20352
                     /gene="pflD"
                     /locus_tag="ACHOWS_002170"
     CDS             18055..20352
                     /gene="pflD"
                     /locus_tag="ACHOWS_002170"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0047736.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGS
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRSGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            20318..21196
                     /gene="pflC"
                     /locus_tag="ACHOWS_002171"
     CDS             20318..21196
                     /gene="pflC"
                     /locus_tag="ACHOWS_002171"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFQ0047737.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSQENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            21198..21539
                     /gene="frwD"
                     /locus_tag="ACHOWS_002172"
     CDS             21198..21539
                     /gene="frwD"
                     /locus_tag="ACHOWS_002172"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFQ0047738.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFDHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21526..22377)
                     /gene="yijO"
                     /locus_tag="ACHOWS_002173"
     CDS             complement(21526..22377)
                     /gene="yijO"
                     /locus_tag="ACHOWS_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0047739.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGATGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22603..24336)
                     /gene="eptC"
                     /locus_tag="ACHOWS_002174"
     CDS             complement(22603..24336)
                     /gene="eptC"
                     /locus_tag="ACHOWS_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFQ0047740.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24519..27170)
                     /gene="ppc"
                     /locus_tag="ACHOWS_002175"
     CDS             complement(24519..27170)
                     /gene="ppc"
                     /locus_tag="ACHOWS_002175"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFQ0047741.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27472..28623)
                     /gene="argE"
                     /locus_tag="ACHOWS_002176"
     CDS             complement(27472..28623)
                     /gene="argE"
                     /locus_tag="ACHOWS_002176"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFQ0047742.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28777..29781
                     /gene="argC"
                     /locus_tag="ACHOWS_002177"
     CDS             28777..29781
                     /gene="argC"
                     /locus_tag="ACHOWS_002177"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFQ0047743.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29789..30565
                     /gene="argB"
                     /locus_tag="ACHOWS_002178"
     CDS             29789..30565
                     /gene="argB"
                     /locus_tag="ACHOWS_002178"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFQ0047744.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30626..31999
                     /gene="argH"
                     /locus_tag="ACHOWS_002179"
     CDS             30626..31999
                     /gene="argH"
                     /locus_tag="ACHOWS_002179"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFQ0047745.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVESKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         7580 a         7938 c         8596 g         8066 t
ORIGIN      
        1 atgccattca gtgaatgaat ggcattataa tttttttatt atttgctaac gtccacccgc
       61 atttcattta cctgtgcagg cgtaatcttg tggcctttct tcaaataatc gatcacgaca
      121 tcggacatgt tatttccggc gcggacctga acatttttcc cttctttgaa tgccagaaaa
      181 ccatcgcccc ccagtgcaag gaaagtcgtt gttgcaacat ggtaaatatt cgtatcaacg
      241 atctcttcac cattaatttt aaaagatact atgcgctgac ctactggttt cttcggggta
      301 tagcgcattt ctgcgccttt tgacatttgc aggatgccat tggtaagcga tgccccgtgt
      361 tccagtaggt tacgcagatc cttaccgctg agatccattt cagtaagttc attcttgaag
      421 gggaatgtac tggtaatgtc gccaaaggta atcgtgcctg catttatatc cgcacgtaag
      481 ctgccggagt ttatcaatgc aatctgtgca gtcggtgcag cgacaagcat tgcatcggta
      541 aacaggttgc caagttgcga tgactcaccg taggcacgag ttaatgcaat cgatgattca
      601 ccgactggtt ggcgtacaag atccgccagt tttttattcc agccattgat aactttttgt
      661 gtggttggat caggtatcca ttcatcggca taaaccgttt tcagctcaaa gctcttcact
      721 ttatgagtac gagctgtagg gttgacatca aggattaatt taccaatatc gataccgcca
      781 ctgtcagtcg aaaggattaa ggtgttgccg actttgattg gttctggcgt tccgacgtgg
      841 gcatgcccgg taatcagaat atccaatcct ttcaccttgc ttgcggtttg aatatcttta
      901 tcaagagcgc gtctaacatc tgtattgcca atgctggact gacgcgccgg tacgccttca
      961 tgaaccagcg ccacggtgag gtcaacctta tcacgtaact catcgatata atgctgcaaa
     1021 tacttcactt catcacgtgc ttcaatcccc tgattcttga gggtcgcgga agatttatca
     1081 aatcggttat tattgtcatt atcgagtccc tggagagaga gctccgagac cgtgtcatta
     1141 aatgcaaata cgccgtgtag cccaacaata ccaatcttga tgccatcttt ttccaggatg
     1201 gtgtagggct tgttccagaa gggtttctca ctctctttat gataaacatt ggctaataaa
     1261 acagggaaat tcgctttgct taattgtcgg agggcattat cccagccatg gtcaaactca
     1321 tgattaccga ttgatactgc gtcaaaaggc atggtattca taatatcaat tattgcttcg
     1381 ccctttgtca ggctactgat atatggcccg gtgaaatagt ctccagcatc aaaataaaag
     1441 gtggctttat ttttggcttt ctcttgtttt actaacgtgg atatattggc aaagccacca
     1501 atgtcgcgtt ttccgtctgc gacatacggt actttatagg aatccacatg agcgtgaaga
     1561 tcattggtat aaataatcgt gacatcctgc gccataactg aggtcgaaaa aatagtcagc
     1621 aatgttgcgg caaataaagt acgctgcgct gttttcattc gcattccttg ttaaataaat
     1681 tgactgataa atgagatttt atttggcgac gtccatgact cgtttagtta agtctggggt
     1741 gacaggttta tgatttttga aataatcgat gatagattcc gccgaggttg ttcccggtat
     1801 cgtcttcaca ttttttccat tcaaaaatgc ttcaaaaccg tcaccaccac tggcacaaaa
     1861 ggagtgcgtg gcaatacgat atgtttgatt atcttccacg ggtttgccgt taatggtgaa
     1921 agagattaac cgttggttga ggggtttttt gctgtcatag acaaccgcaa cacttttaga
     1981 aacttgtaac acaccgttag ttagattggt tgcatgcgtc atcaatgatt tgagatcgcg
     2041 gcccgtcaaa tccatctcga tgaggtcatt attaaagggg aatgtgctaa taacatctcc
     2101 cagtgtgata tcgcctttag gaaaatcagc tcgaatccca ccgctatttt gaaatccggc
     2161 aacggcatca ggtgtttttt ccatcattgc atcaagaata aggttgccgg tagaagagga
     2221 actaccatac tcacgtgtta aaactacggg agaatggccg acaacttcct gtgttatttt
     2281 gttaagcttt gcactccatt tatcaatagt attttgaacg atggtgtcag gtttaaattg
     2341 atctgcaaag atggtgatta actcaccatt ataactgtca attttcttcg ttttcggatt
     2401 gtaatcaagc acaagttttc cgatgttggt gccgtatgca tcggtagata caattaatgt
     2461 gttattaact tttattggtt gcggtgttcc gacatgtgcg tgaccggtaa ttaagacatc
     2521 aacaccattc acttttttag ctgtctcaat atctgcctgt agtagccgct ccacatcttt
     2581 actaccaaag cttgattgac gcgccgggac accttcgtga attagcaaaa cggtaatgtc
     2641 gactttacct tttaattctg caagtgcttt atttaaatat ttaatttcgt ctctggcttc
     2701 tacgccttca cgtgcttttg ccgcgactgt atcatagaaa gcgaatgcgc catgcaatcc
     2761 aatcacacca atctttaaac catctttttc gataatggtc cacggatgat cccaaactgg
     2821 tttattcgta tcggtgtagt agatatttcc cagcagaatg gggaattttg cttttgataa
     2881 ctgagacacc atattgggca caccgtgatc gaactcgtga ttaccaacag acaccgcatc
     2941 aaagggcata gtgttcatga tatcaataat agcctcacct ttggtcaggg tgctgatata
     3001 cggcccggta aaatagtcgc ctgcatcaaa gaaaaacaca tctttgcttt ttttcttcgc
     3061 ttcattaacg atgcctgaaa tattagccat accacctata agtctgttct tatcaacagc
     3121 gatgattttt gctggattga catgtgcgtg aatgtcattc gtataataaa tagtaacgtc
     3181 ctgagctaga acactgtgag aaaatatcac gccgaatatg actgataatg ttgtaaatac
     3241 agttttcatc atccctcctg gatagggtgt gttacatttg ttgaattatt taaataaaag
     3301 acacatcgtg tcttatttta tgtaagcata gttcagaaaa gtattgtgtg atcttagtaa
     3361 caaataatgt tattaattaa acattgacca tgattatatt tcacggtcat acatttatat
     3421 atttaattaa atgaaaggta gtgttctctc ttaatttatg gtcgtttgtc gagtgtattg
     3481 actaacaaga tggagtgagc gattcattgt attttaacgc cagtgaggtc agcatttgtg
     3541 ttacgcgacc tgcggataat aacgcgcttt gggtaaagtc gtgttggtct gtcagccatg
     3601 tgataaaaca acgactggtt gccagagaaa catttgcgcc cctttctaag caggctcccg
     3661 tgagtaggca gaccatcttg cagttattcc cctgctccca gcgctgccac tgattttgcc
     3721 accaaccatg gtatgtcgtt cccttaaaat gccagggcgt atcagcgtta ctgtcactga
     3781 tttcttcttc tacgaaccaa agttcacctt gttgttggcg gaactgatct gcccaggctg
     3841 taataattgc gcctcttaga aaacattcgt gtgaaataat atgcgttttg acatctggat
     3901 agcggatttt cagtattctg aaatgcccgt gtgccgtgat aaccgggcgt agtatggctc
     3961 ccttaccgtt gcgcttgagt acagcactgc catactgaat ttcatcgaac tggctccggc
     4021 gcaagaattg cgcttcacgc atatctacct tctgcacgat atcgcgatgg tgcatagttg
     4081 gttctgtgtt gcccttgtaa ctgtaccgca gactgtctcc tgtctgatgt tgcgtataat
     4141 tcgtggagag atgaagaatg ttgccacgag tggcgtcaag actgactaaa acatacagtt
     4201 tttgtactgc gctggcccct tgaaagggta tgaacagttg aacggtttca atggcctggg
     4261 gatgcgtaat ttctttctga tactgtttag gcgtccagac ttttttgcag ttacggcatt
     4321 gaatgcgttg cgaaccctgt ggattgtgac cataacaaat catgtctgtg ccataacata
     4381 ccgggcagaa atgacccttc tccgaggccc acgtagataa gtgaactgac aaccagtcac
     4441 gaaattgttg ctcgtcaaac aacggcgggt aactgccaca tgcccgacag tgcagtgccg
     4501 gataacccag tttatagtct ggccaactgt aatcttccga tgaggccaat cccaaatttc
     4561 ggcaaccgaa cgttttacag gcattaatcg taaacagagt cgacatatat caccaacgat
     4621 ttcacaaggt cgttgatttt gtccggcaac tatcccctgc agatgtgatt actctcacat
     4681 aaataagtct ttcagaacgt aatgtagcaa tcctttatct cactgaaagg gattgtttat
     4741 gaaaattaaa atacttgctg cgggtatcgc attaacactg ccgttctggg cttgcgctaa
     4801 agatgtcact attatttata ccaacgacct ccatgcgcat gtagaacctt ataaagtgcc
     4861 gtggatcgct gacggtaaac gggatattgg cggctgggca aatatcacta cgctggttaa
     4921 gcaagaaaaa gcaaaaaata aagcgacatg gttttttgat gcgggcgact attttaccgg
     4981 gccatatatc agcagcctga ccaaaggtaa ggctattatc gatattatga atactatgcc
     5041 gtttgatgcc gtcacgatag gcaaccatga atttgaccac ggctgggaca acacattatt
     5101 acagttgagt caggcaaaat tcccgattgt gcagggcaat gttttttatc agaacagcag
     5161 taaatcattc tgggataagc cctataccat catcgaaaaa gacggtgtga aaataggcgt
     5221 tataggtttg cacggtgtat ttgcctttaa tgacacagta tctgctgcca cgcgagtggg
     5281 tattgaggcg cgtgatgaaa ttaaatggct acaacgttat atcgatgaac ttaaaggcaa
     5341 ggttgatttg accgtcgccc tgatccatga aggtgttccg gcccgccagt ccagtatggg
     5401 aggcacagat gtgcgtcgcg cgctggataa agatattcag acggcaagtc aggtgaaagg
     5461 gctggatatt ttgatcaccg gacatgcaca tgtgggtacg ccggaaccga ttaaagtcgg
     5521 caatacgctc atcctctcca ctgacagcgg cgggattgat gtgggcaaac tggttctcga
     5581 ctacaaagag aagccgcata attttacggt gaaaaacttc gagcttaaaa ccatttacgc
     5641 tgacgagtgg aagcccgatc cacaaacgaa acaggtgatc gacggctgga ataaaaagct
     5701 tgatgaagtg gtgcaacaaa cggtggcgca atcgccggtt gaattaaaac gtgcctatgg
     5761 tgaatcggct tccctcggga acctggcggc tgacgcttta ctggtagcgg cgggtaaaaa
     5821 cacccaattg gcgttaacca actctggcgg cattcgcaat gagatccccg caggggcgat
     5881 cacgatgggc ggcgtcatca gtacgttccc gttccccaac gaactggtga cgatggaact
     5941 cacgggtaaa caattacgca gtctgatgga acacggcgca agtttaagca atggcgtttt
     6001 acaggtatcg aaaggcctgg aaatgaagta cgacagcagc aagccggttg gtcagcgggt
     6061 aatcacactg actctgaatg gcaaacccat tgaagatgcg acggtttatc acattgctac
     6121 tcagagtttc cttgctgatg gtggagatgg ttttaccgcc tttaccgaag ggaaagcgcg
     6181 taacacaacg ggcggttatt acgtttatca cgcggtggtt gattacttca aagcgggcaa
     6241 caccattacg gatgaacagc tcaacggtat gcgcgtgaag gatatcaaat aaaagtgcgc
     6301 actgaattga ctcaactgtt tgttacccgt tgattgcgca gatcctgtat tcggcctacg
     6361 cgagggcgca ggccgaatca gatgccttag catcaccatc cggtggcagg acgttgtttt
     6421 tcgaattagc ccaggtgtcg ctgtgttgtc agaatatgag tcaaaacgtg cgaggtggat
     6481 tgtgctatag agaaatcttt tatcgcctct aattaagatg agtttttttc atcttccctg
     6541 atttttcctc accatcattg gttatttttc ggttgacgcc cttcggcttt tccttcatct
     6601 ttacatctgg acgtctaaac ggatagatgt gcacaacaca acatataacg ataagcgatt
     6661 gatgaggtaa ggtatgagct tttttcacgc cagccagcgg gatgccctga atcagagtct
     6721 ggcagaagtc caggggcaga ttaacgtttc gttcgaattt ttcccgccgc gtaccagtga
     6781 aatggagcag accctgtgga actccatcga tcgccttagc agcctgaaac cgaagtttgt
     6841 atcggtgacc tatggcgcaa actccggcga gcgcgaccgt acgcacagca ttattaaagg
     6901 cattaaagat cgcaccggcc tggaagcggc accgcatctt acctgtattg atgcgacgcc
     6961 tgccgagctg cggaccattg cacgcgacta ctggaataac ggtattcgcc atatcgtggc
     7021 gctgcgtggc gacctgccgc cgggaagtgg taagccagaa atgtatgctt ctgacctggt
     7081 gacactgtta aaagaagtgg cagatttcga tatctccgtg gcggcgtatc cggaagtaca
     7141 tccagaagcg aaaagtgccc aggccgattt actcaacctg agacgaaaag tggatgccgg
     7201 agccaaccgc gccattactc agttcttctt cgatgtcgaa agctacctgc gttttcgcga
     7261 tcgctgcgtc tcggcaggca ttgatgtgga aatcattccg ggaattttgc cggtttcgaa
     7321 ctttaaacag gcgaagaaat ttgccgacat gaccaacgtg cgtattccgg cgtggatggc
     7381 acaaatgttc gacggcctgg atgacgatgc tgaaacccgc aagctggtgg gcgcgaatat
     7441 tgccatggat atggtgaaga ttttaagccg tgaaggagtg aaagatttcc acttctatac
     7501 gcttaaccgc gctgaaatga gttacgcgat ttgccataca ctgggtgttc gacctggttt
     7561 ataaatagtg tgccttttgt gaaaattaca cagtgatcac aaattttaaa cagagcacaa
     7621 aatgctgcct cgaaatgagg gcgggaaaat aaggttatca gccttgtttt ctccctcatt
     7681 acttgaagga tacaaatcta aaaccctttt ttataaagca tttgtcctaa atcggacata
     7741 atcaaaaagt cttaattacg atcaatttga tctacatctc tttaaccaac aatatgtaag
     7801 atctcaacta tcgcatccgt ggattaattc aattataact tctctctaac gctgtgtatc
     7861 gtaacggtaa cactgtagag gggagcacat tgatgagcac gtcagacgat atccataaca
     7921 cgacagccac cggcaaatgc ccgttccatc agggcggtca cgaccagagc gcaggtgggg
     7981 gcacaaccac gcgcgactgg tggccaaatc aactccgtgt tgacctgtta aaccaacatt
     8041 ctaatcgttc taacccgctg ggtgaggact ttgactaccg caaagaattc agcaaattag
     8101 attactacgg actgaaaaaa gatctgaaag ccctgctgac agaatctcaa ccgtggtggc
     8161 ctgccgactg gggcagctac gccggtctgt ttattcgtat ggcctggcac ggtgcgggta
     8221 cttaccgttc aatcgatggg cgcggcggcg cgggtcgtgg tcagcaacgt tttgcaccgc
     8281 tgaactcctg gccggataac gtaagcctcg ataaagcgcg ccgcctgttg tggccaatca
     8341 aacagaaata tggtcagaaa atctcctggg ccgacctgtt tatcctcgcg ggtaacgtgg
     8401 cgctggaaaa ctccggcttc cgtaccttcg gttttggtgc cggtcgtgaa gacgtctggg
     8461 aaccggatct ggatgttaac tggggtgatg aaaaagcctg gctgacccac cgtcatccgg
     8521 aagcactggc gaaagcaccg ctgggcgcaa ccgagatggg cctgatttac gttaacccgg
     8581 aaggcccgga tcacagcggc gaaccgcttt ctgcggcagc agctatccgc gcgaccttcg
     8641 gcaacatggg catgaacgac gaagaaaccg tggcgctgat tgcgggtggt catacgctgg
     8701 gtaaaactca cggtgccggt ccgacatcaa atgtaggtcc tgatccagaa gctgcaccga
     8761 ttgaagaaca aggtttaggc tgggcgagca cttacggcag cggcgttggc gcagatgcca
     8821 ttacctctgg tctggaagta gtctggaccc agacgccgac ccaatggagc aactatttct
     8881 tcgagaacct gttcaaatat gagtgggtac aaactcgcag cccggctggc gcaatccagt
     8941 tcgaagcggt agacgcaccg gaaattatcc cggatccgtt tgatccgtcg aagaaacgta
     9001 aaccgacgat gctggtgacc gacctgacgc tgcgttttga tcctgagttc gagaagatct
     9061 ctcgtcgttt cctcaacgat ccgcaggcgt tcaacgaagc ctttgctcgt gcttggttca
     9121 aactgacgca cagggatatg gggccgaaat ctcgttacat cgggccggaa gtaccgaaag
     9181 aagatctgat ctggcaagat ccgctgccgc agccgatcta caacccgacc gagcaggaca
     9241 ttatcgatct gaaattcgcg attgcggatt ctggtctgtc tgttagtgag ctggtatcgg
     9301 tggcctgggc gtctgcttct accttccgtg gtggcgacaa acgtggtggt gccaacggtg
     9361 cgcgtctggc attaatgccg cagcgtgact gggatgtgaa cgccgcagcc gttcgtgctc
     9421 tgccagttct ggagaaaatt cagaaagagt ctggcaaagc ttcgctggcg gatatcatcg
     9481 tgctggcagg tgtggttggc gttgagaaag ccgcaagcgc cgcaggtttg agcattcatg
     9541 taccgtttgc gccgggccgc gttgatgcgc gtcaggatca gactgacatt gagatgttcg
     9601 aactgctgga gccaattgct gacggtttcc gtaactatcg cgctcgtctg gacgtttcca
     9661 ccaccgagtc actgttgatt gataaagcac agcaactgac gctgaccgcg ccggaaatga
     9721 ctgcgctggt gggcgggatg cgcgtactgg gtgccaactt cgatggcagc aaaaacggcg
     9781 tcttcactga ccgcgttggc gtattgagca atgacttctt cgtgaacttg ctggatatgc
     9841 gttacgagtg gaaagcgacc gacgaatcga aagagctgtt cgaaggccgt gaccgcgaaa
     9901 ctggcgaagt gaaatacaca gccagccgtg cggatctggt ctttggttct aactctgtcc
     9961 tgcgtgcggt ggcggaagtc tacgccagca gcgatgccca cgagaagttt gttaaagact
    10021 tcgtggcagc gtgggtgaaa gtgatgaacc tcgaccgttt cgacctgctg taatctgacc
    10081 ttcttcagcg actgcctttc atgcagtcgc tgaagtttct ttaccggcgt atagtgtcca
    10141 caggaaaact acacactgga tctctcatgt ctgccgcagg aaagagcaac ccactggcaa
    10201 tcagtggcct ggttgtgctc acacttatct ggagttatag ctggattttc atgaagcaag
    10261 tcaccagcta catcggtgcc ttcgacttta ccgccttacg ctgcattttc ggcgctctcg
    10321 ttttattcat cgtcctttta ttacgtggtc gcggaatgcg cccgacaccg tttaaataca
    10381 ccttagccat tgccctgtta caaacctgcg ggatggtcgg cctggcgcag tgggcgctgg
    10441 tcagcggagg agcggggaag gtggcaatcc tgagctatac catgccgttc tgggtggtaa
    10501 ttttcgccgc gttgtttctc ggtgaacgcc tgcgacgcgg acaatatttc gcgattctga
    10561 ttgccgcttt cggcttattt ttggtgttgc agccgtggca actcgatttc tcttcgatga
    10621 aaagcgccat gctggcaatc ctctccggtg tgagttgggg ggcgagcgcg attgttgcta
    10681 aacgtctgta tgcccgccat ccgcgcgtgg atttattgtc gctaacatcc tggcagatgc
    10741 tgtatgcggc gctggtgatg agcgtggtcg ctttactggt gccgcaacgt gaaattgact
    10801 ggcaacccac cgtgttctgg gcgctggcct acagtgcgat tctggcgaca gcgctggcgt
    10861 ggagcttatg gttgtttgta ttgaaaaact tgcctgccag tattgccagc ttaagcacac
    10921 tggccgttcc cgtttgcggc gtactctttt cctggtggct gctcggtgag aatccggggg
    10981 cagttgaagg tagcggtatt gtgctgattg tactggcact ggcgctggtg agccgtaaga
    11041 aaaaagaagc cgtcagtgta aaaaggatct gaattttttc ttcatgtggg gagatctctt
    11101 atttagcaaa ataccgatag tgccccacca tccgccagct aaacagtaca tcttcttcct
    11161 gcgcgcctgc gccaatgtta tgtatcacca gcggcgtacc gtcgcgggcg aagccatctg
    11221 acaccacccc aatatgtgcc agcccgttat ctaatcgcca ggagacaata tcgcccgctt
    11281 gatagtcact ggggttcttg ctggtggggc gtgttttatc gtggcgggta aaccaggttt
    11341 ccagattagg cacccggcgg tgatcgatgt tgctgtccgg gcgctttaac tgccattttt
    11401 gcgggtacgc agcaaaattc ttcgccatat cttcgtgcac cagtttctgc aaatcgacct
    11461 tctggctgcg caatgcacgg atcaccacat cggaacatac gccgcgttct tgcgggacat
    11521 caccgccagg ataggtaagc tgcacatacg ccggatcgta aaatagcgtg ctgccgattt
    11581 gctgtctggc gccgtctgcg atggcaagat tggtgttggc ctggatttgt accacggttg
    11641 gtggaacggc gggagatttt aaggagtaac tggtaaatac cgtcagaaga ctgaataacg
    11701 ccagtgaagc tttcatctgt ttcgtccgtg aaaataagag tcacagattc gaccttccgg
    11761 gctaacgatt gttctgtgga gaaaatgtcc agaagcggat aaaaaaacac ccgaacaagg
    11821 tggaagtttc agattactgg cacagcgtgc cggatgcggc gtgaacgcct tatccggcct
    11881 acaaaagtac gtaaatttac tatattgcag agattgcgta ggcctgataa gcgtagcgca
    11941 tcaggcaatt ttgcgttcaa actcccggac aagccgggag tttggagtag gttattccca
    12001 ctcttgcagg aaacgctgac catactggtc ggctaccagc agtgcggcgt aaacctgatc
    12061 tggcgtcgcg ccgccaggca tgttgtggat ggtttcacct tctgcacatg ccgcttctgc
    12121 cacaattcgc attttcgccg ggacatcttc tttaatatcc agttgagcga gagttattgg
    12181 caaacctacc gcatggctaa gtgcagctac ggtttcgatt tcctccaccg gcgcgttttc
    12241 cagaaccagc tgcgtcagcg taccaaatgc cactttttca ccgtggtaat agtgatgcgc
    12301 gtccgggata gcggtcaggc cgttatgcac cgcgtgagcc gccgccagcc cgccgctttc
    12361 aaaaccaacg ccgctcaaat aggtgttcgc ttcaatcacg cgctccagcg ccggggttac
    12421 tacatgctgc tcggcggcaa gcatcgcttt ttcgccttct tccagcaggg tgttgtagca
    12481 cagttcagcc agtgccagcg ccgcctgggt gcacttgccg cccgccatgg tggtcgcgcc
    12541 gctacgagag caagcacgcg cttcaaacca ggttgccagc gcatcgccga tacccgccgc
    12601 taacagacgt gcaggtgcgc cagcgacgat tttggtgtcg acaatgacca tattcgggtt
    12661 atttggcaac atcagatagc ggtcaaattc accatcatcg gtgtagataa cagacaatgc
    12721 gctgcacggt gcgtcggtag aggcgatagt cggtgcgatc gctaccggaa cacccatgaa
    12781 atgtgccagt gctttggcag tatcgagagt ttttccgcca ccgataccga gaattgcgcc
    12841 acactgcgca gtctccgcga tgccacgcag acggtcgatc tcattttgcg aacattcacc
    12901 gccaaacggc gcaatttcta ctaccagtcc agcatcttta aagcttttct cgacagtgga
    12961 ttgagcaaaa cctaaaacaa atttgtcacc caccactaac cagcgttctg ccagcggctt
    13021 caggtattcg cccagacgat taatcacatc agcgccctgg atgtatttac ccggtgattg
    13081 aataatgcgg tccataattg ctcctttaga gatgagtagt gccaaatgcg gcattccagt
    13141 cgtgttcgaa cttctctata gctgactcta ccgcaggggt gttgagcatt tgttgcgcta
    13201 catctaaggg cagggtgatg gattcacatc ctgccagtaa acagtccagc gcctgacgtg
    13261 gtgttttaaa gctggcagcc agcaccatgc tttctggcgc atgcatttcc aacagcgctt
    13321 gcagctcctg tacagtacga atgccgtcac cgccctgggc atctacgcgg ttaacgtatg
    13381 gagcaacgta tttggctcca gccagcgccg ccagtaatcc ttgcgctgcg ctgtacactg
    13441 cggttcccag cgtagtaatg ccttctttct tcagcatttt aattgctgcc agaccttcag
    13501 aggttaccgg aattttaacc acaatgcccg gaatagcgtc gcgcaggcgt ttcgcttctt
    13561 tcaccatacc ctgcgcgtcg cggctcatgg tctgagcaaa cagaattccc tcatcaccga
    13621 tcgctttttg aaggcgcggc agcacttccc agatggactc cttgctggca gcgataatgc
    13681 tcgggttagt tgtcaccccg gcaatcggga atatgcgtgc cagacgttcg acttctgcga
    13741 cgttagcggt gtccagatac agttccatga tgtttccttt tacagttcca gttcatgttg
    13801 cagcaggctg gcgatagcgt cggcagaggc ggcgttaacc agcgcgttac ggaattcttc
    13861 gtgcatgatg cgacgagcga ggcgcgagaa aatgcgcatg tgctgatcgc ccgcagcatg
    13921 tttgttcagg gttaacataa tgatgaattg cgcttcatca tcgccccagc gcaccggcgc
    13981 tgccagacgc gccacgctga tggtggattg ctcaatgtgt tctgatttgc tgtggggaat
    14041 ggcaaaacta aagcccagac cggtagagaa aacggcctcg cgcgcccaca agtcggcttc
    14101 cagtttacgc ggatagcgac agcggcccgc cagcagcagg ttatcggtca tgcctttgag
    14161 cacttcttct ttgctgcgcc agtcgctttc cagtgtgatg cactcggcgg tgaccagcgg
    14221 tgcgtcttgt tgggtcatgc ggaattgcgc cagcaggtgt tccacttcca gcgaagtacg
    14281 gcaggccatt gcctggttga gcaacttgcg gcactcacgg ctatcaagtt gcgccatccg
    14341 cgctttcgcc gccggaattg atggtgcgct catgctgagt tcatccagcc ctaaaccgac
    14401 cagcaacggc agcacggaac ctttcgctcc cagttcaccg cacagaccaa tccatttgcc
    14461 ctggcgatgc accgcctgca cggcgtaatc gagcgcccgc aagaatgctg gattcaggct
    14521 gttgtagtga cgagtcacct tagcgttatc gcgatccact gccagtagat attgcgtcag
    14581 gtcattacta ccaatactaa agaaatcaat ctcttcgcag cattgatcga tgatgaacat
    14641 caccgacggc acttccagca tgatgccgag tggaatcttc tcatcaaacg gaatgtgttc
    14701 gttacgcagt tgctgtttgg cttccgccag tttttctttc acccataaga tctcttccat
    14761 tgaggagatc atcgggatca tgattttcag gctgccgtga gcagaggcgc gcaggatcga
    14821 ccgtagttgt gtggtaaaca acgacgcgta ttcttcataa atacgcaccg cgcgataacc
    14881 gaggaacggg tttgcctctg cgggaatatt cagataatca acgggtttgt caccgccaat
    14941 atccatagtg cgcacaataa tgctgcgtcc gttggcggac tccagcgcct gacaaaaaat
    15001 gttgtacagc tcgctttcgc ccggtgcact ggtgcgatcc atatagagca tttcagtgcg
    15061 gaataaacca acgccttccg caccattgcc gaatgcggcc tgcgcttcca cggagtgagc
    15121 gatgttagcg gcaatttcaa tgcggatacc gtcagcggta cgggcttgtt gggtcagcca
    15181 gacacgctgt tgctcacgca gggcgtcctg tacgcgggct tcttgctgat aataacgggc
    15241 cacggcttcc cctggctcaa ccacaatcgc ccctgcgttg ccgtcgatat aaatcgtttg
    15301 atgctgccac ggagtaaggg catcaatatc cacacctacc agcgtcggaa tgttgaacga
    15361 acgggcaagg atcaccgtat gtgaggtggt gccgccactt ttcagtaata gacctttgag
    15421 gtgattttta tccagttcga ggaactggct gggggtcagt tcatcagcca tacaaatggc
    15481 gggctgcgtc agtttgcccg gtgccgggaa gcgttgctca ccgtagattt gctggagtaa
    15541 ctggaagcag acgtcgcgta cgtccagggc acgttcttgc aggtagctgc tgctggaacg
    15601 ggcgaactct tcgcaaaagt gattcgcact ggcaacaatc gcttcggcgc agcttaatcc
    15661 ggcgctgaca cctgccagta aatgttcgcg cagggaagta tcgccagcca gggatcggtg
    15721 agcttccagt atcgcgctgg tagcaccgtc gctatccagc agacgaaatt caatgttttt
    15781 catcaccagt gtcaggccgt tttccagcgc ggattgctcg gcgtcaacgc ctttggcagc
    15841 aggaagatta cccagcgcat tgagatctaa agaagagatc ggcgtcagaa tgccgcctgc
    15901 actaccactg cacacggtgc gagcacggat aatttgcgga ttcagattgg tcagtgaaac
    15961 cggcagtggt tccagttcgt cagattttat ttccgccagc ggtgcgtcgc agtgggggaa
    16021 ttcatcgcgc agccattggc ttaaacgctg gtgcgcttcc tgttcgtcgg ccccggaaat
    16081 cagtaactgg cagttatcgc ccaccagcgt atcggtgcca atcagcgcaa gggcgctttt
    16141 ggcgttgccc ttgcggtcag tgcgcaggtt atgccactca atttgtgatg aaaaagtatt
    16201 acacagcgtt tcaacgtggc ttgccggacg tgcatgtacg ccgttaggta gctcacaaat
    16261 aaattccaca atcagggcca ttgcctctct cccggtacgg ttttctgttt acaggataag
    16321 agcgcacggc aacggcctgc catgtgacaa atctgccaaa agctggacaa atgtaatgta
    16381 accgtcaatt tgcgacgcgt ctcacaagac gccgttttgc ggcatgcttc cggatcatcg
    16441 caagttatga ggcggatcgc atttttgtac tgatattaca aaagtccagt aaatggcctt
    16501 tttatccact gtttgctccg cctgcgattg cctattgttt cctccatcaa aaatatggac
    16561 atgggctttt gcaagcccgg gagcagctta tgaatgagtt ggtgcagatc ctgaaaaata
    16621 cccgtcagca tttaatgacg ggcgtttcac acatgattcc cttcgtggta tcgggcggta
    16681 ttttgctggc ggtttccgtc atgttgtatg gcaaaggcgc agtgccggat gccgtagccg
    16741 atccgaatct gaaaaaactg tttgatatcg gcgttgcggg tttgacgctg atggtgcctt
    16801 tcctcgccgc ttacattggt tactccattg cagagcgttc tgcgctggct ccgtgcgcta
    16861 tcggtgcctg ggttggtaac agctttggtg cgggcttctt tggtgcactg atcgccggga
    16921 ttatcggcgg catcgtggtg cattacctga agaaaattcc ggtgcataaa gttctgcgct
    16981 cggtgatgcc aatcttcatc attccgatcg tcggcacact gattaccgca ggcatcatga
    17041 tgtgggggct gggcgagcct gtgggggcat tgaccaacag cctgactcag tggcttcagg
    17101 ggatgcagca gggcagcatt gttatgctgg cggtgatcat gggcctgatg ctggcgttcg
    17161 atatgggcgg tccggttaac aaagtggcct atgccttcat gctgatttgc gttgctcagg
    17221 gtgtttatac cgtggtggcc attgctgccg tgggtatttg tattccgccg ctggggatgg
    17281 ggctggcgac gctgattggt cgtaaaaatt tctccgcaga agagcgcgaa accggtaaag
    17341 cggcgctggt gatggggtgc gttggggtta ctgaaggggc gattcctttc gccgctgccg
    17401 atccgctgcg tgttattcct tccatcatgg tcggttcagt ttgtggtgca gtaactgcgg
    17461 cgctggtcgg tgcgcagtgc tatgcaggct ggggcggtct gattgtgctg ccggtggttg
    17521 aaggcaagct gggttatatc gcagcagtag ctgtcggagc agtggtgacg gctgtttgtg
    17581 tgaacgtgct gaaaagtctg gcgcgtaaaa atgggtcttc gactgatgaa aaagaagacg
    17641 acctggattt ggattttgaa attaattaat tgaggaacga gccatgacga aaattattgc
    17701 agtaaccgcg tgtccatctg gtgttgccca tacctatatg gcggcagagg cgctggaaag
    17761 tgccgcgaaa gcgaaaggct gggaagtgaa agtagaaacc cagggctcga ttggtctgga
    17821 aaacgaactg actgcggaag acgtggcgag cgccgatatg gtgatcctga ccaaagatat
    17881 cggcatcaag tttgaagagc gttttgctgg caagaccatc gtgcgcgtca acatcagcga
    17941 cgcggtaaaa cgcgctgatg ccattatgag caaaattgaa gctcacctgg cacaaaccgc
    18001 ttaagttctt atcccgcccg taagggcggg ttcgctttcc cacaggagtt cctcatgacg
    18061 aatcgtatct ctcgcctcaa aactgcactg tttgccaata cccgtgaaat ctcgctggag
    18121 cgtgcgctgc tttataccgc cagccatcgg caaaccgaag gcgaaccggt gatattgcgc
    18181 cgggcgaaag caacagcgta tatccttgaa catgttgaaa tttcgattcg tgatgaagaa
    18241 ctgattgccg gtaaccgcac cgtaaaaccg cgcgccggga ttatgtcgcc ggaaatggac
    18301 ccttactggc tgctgaaaga gctggatcaa ttcccgacgc gtccgcagga ccgctttgct
    18361 atcagcgaag aagataaacg tatctaccgt gaagagttgt tcccgtactg ggaaaaacgt
    18421 tcgatgaaag atttcattaa cgggcagatg acggatgaag taaaagccgc gaccagcacg
    18481 cagattttca gcatcaacca gacggataaa ggccaggggc acattattat tgattacccg
    18541 cgcctgctga atcacgggct gggtgagctg gtagcgcaga tgcagcaaca ttgtcagcaa
    18601 cagccggaga atcactttta tcaggcagcg ctgttactgc tcgaagcctc gcagaaacac
    18661 attttgcgtt acgccgaact ggcggaaacg atggcggcaa gctgcactga tgcccagcgt
    18721 cgcgaagaac tgctgactat tgcggagatc tcccgccata acgccgaaca taagccgcag
    18781 acgttctggc aggcgtgcca gttattctgg tacatgaaca tcattctgca atacgaatcc
    18841 aacgccagtt cactatcgtt ggggcgcttc gaccagtata tgttgccgtt ctatcaggca
    18901 tcattaaccc agggcgaaga tccggcgttc ctgaaagaac tgctcgaatc tttatgggtg
    18961 aaatgcaacg acatcgtgct gttgcgctcc accagtagcg cgcgttattt cgccggtttc
    19021 ccgaccggct ataccgcact gctcggcggg ttaaccgaga acggacgtag cgcggtgaac
    19081 gtgctttcgt tcctttgcct tgacgcttat caaagcgtgc aattaccgca accgaacctc
    19141 ggcgtgcgca ctaacgcctt gatcgacacg ccgttcctga tgaaaaccgc agaaaccatt
    19201 cgcctcggca ccggtattcc gcaaatcttt aacgatgaag tggtggtgcc agcgttcctc
    19261 aaccgtggcg tttcgctgga agatgcacgc gactattccg tagtgggctg tgtggaatta
    19321 tctattcccg gcagaaccta cggcttgcat gacatcgcga tgtttaatct gctgaaagtg
    19381 atggaaatct gcctgcatga aaatgaaggc aatgctgcgc tgacttatga aggtttactg
    19441 gagcagatcc gcgccaagat cagccactac atcaccctga tggttgaggg cagcaatatt
    19501 tgcgatatcg gtcaccgtga ctgggcacct gtaccgctgc tctcatcgtt tatcagcgat
    19561 tgtctggaaa aaggcagcga tattaccgat ggcggcgcgc gttataactt ctccggcgta
    19621 caggggatcg gtatcgccaa cctgagcgat tctctccatg cgttgaaagg gatggttttt
    19681 gagcaacagc gtttaagttt tgacgaattg ctgtcggtat taaaagccaa cttcgcaacg
    19741 ccagaaggcg aaaaagtccg tgctcgctta attaaccgct tcgagaaata cggtaacgat
    19801 atcgacgagg tggataacat cagcgccgaa ctgttacgcc actactgcaa agaagtggaa
    19861 aaataccaga acccgcgcag cggctacttc acgccgggat cgtataccgt ttctgctcac
    19921 gtcccgttgg gatcggtggt tggcgcgacg ccagacggtc gttttgccgg ggaacaactg
    19981 gcagacggcg gcttgtcacc catgctgggc caggacgcac aagggccaac ggcggtactg
    20041 aagtcagtca gtaagctcga taatacgctg ctgtctaacg gtacgttgct gaacgtgaaa
    20101 ttcactccgg cgactctgga aggtgaagcg ggattacgca aactggccga cttcttacgg
    20161 gcgtttaccc agcttaagtt gcagcatatt cagtttaacg tggtgaacgc cgacacgttg
    20221 cgggaagcgc aacagcgccc acaagattat gccgggctgg tggtgcgcgt tgccggatac
    20281 agcgccttct ttgtcgaatt gtcgaaggag atccaggatg acatcatccg ccggacagcg
    20341 catcagctgt aacgttgtgg aaacgcgccg caatgatgtg gcgcgcattt tcaacattca
    20401 gcgttattca ctgaatgacg gcgagggcat tcgtacggtg gtctttttta aaggctgtcc
    20461 gcatctttgc ccatggtgtg ctaatccgga gtcgatctcc ggcaaaatcc agacggtacg
    20521 cagagaggcg aaatgtctgc actgtgcgaa atgtttgcgt gatgcggatg aatgcccctc
    20581 cggggcgttt gaacggattg gccgcgatat cagccttgac gcgctggagc gggaagtgat
    20641 gaaagatgac attttctttc gcacgtccgg cggcggcgtc acgctttctg gcggcgaagt
    20701 gttaatgcag gcggagtttg ctacccgttt tttacagcgg ctgcggttgt ggggcgtgtc
    20761 atgcgccatt gaaactgccg gagacgcgcc agccagcaag ctgttaccgc tggcgaaatt
    20821 gtgcgatgaa gtgttgttcg atttaaaaat tatggacgcg actcaggcgc gggatgtggt
    20881 gaagatgaat ctgccacgcg tgctggagaa tctgcgtttg ctggtgagtg agggcgtcaa
    20941 cgtgatcccg cgtttaccgc tgatccctgg tttcacgctc agccaggaga atatgcagca
    21001 ggctctggac gtgctgatcc cgctgaatat caggcagatc catctgttac cgtttcatca
    21061 gtacggcgaa ccgaaatacc gcctgctggg gaaaacatgg tcgatgaaag aggtgcctgc
    21121 gccgtcgtca gctgatgtgg caacgatgcg cgaaatggca gaacgggccg gatttcaggt
    21181 taccgtggga ggttaaaatg gcatacctgg tggcagtaac cgcctgcgtc agtggcgtgg
    21241 cgcatactta tatggcggcg gaacggctgg aaaagttgtg cctgttagag aagtggggcg
    21301 tcagcattga aactcagggc gcgctgggaa cggagaatcg tttagcggac gaggatatcc
    21361 gccgggcgga tgttgcgctg ttaattacgg atatcgagct tgccggtgct gagcgatttg
    21421 accattgccg ctatgtgcaa tgcagcatct acgctttcct tcgtgagccg cagcgggtga
    21481 tgagcgcggt acgcaaagtg ctttctgcgc cgcagcaaac ccatctcatt ctggagtagt
    21541 cggtttttct gtcagttggc tgtggtactg ccgacgatat tccgacggtg agcgttcggt
    21601 atttttacga aacagacggc agaagtagtt gctgtcgaca aagccgcagg cgtgcgccac
    21661 ttcttttacc ttcaggtcat agcctttcaa taacgtctta gcgtgctcca gtcgcgtgtg
    21721 attcaggtat tcgttaaaac cagtggcccc cgttttttga aacaggtgcg agaggtaatt
    21781 tggcgaaata tagaatgcct gtgcgacaga ttcgcgggta agcgcggagg catagcgttc
    21841 gtcgatataa tcacgaatag cttcgaacag tgcctggctg cgtgaggcgg tctggatttg
    21901 gctgccaagc aaatcgcggc agtggctaag caggctggcg acgataagcc ttgccgtttg
    21961 ctgctcctgc gactgcattt gcatttcatt gagcgtttgt agcagaaaag aacctatgcg
    22021 tgggccgcgt cgggcgacgt gttgcttcgc cagattttga tattgtttgc catcccattg
    22081 cacgacgctg aagccgagtt gctgtttgcc aaacagcacg ctaaaggtag tggcgggggc
    22141 ttgccattgt ggaaaattcc agccaccagc gggaacgtac agcacatcgc cgggaactaa
    22201 cgctgctcca gcgccggtat caataaactc accttccagc acaatttcca gccgtggaaa
    22261 atcgacctga tacgcaagat cgggcaccgg gccgttgctg ctggcaaagt aaatctggcg
    22321 cagggacagc gggccgttga tcaggcggga gagcagatag ctgacgtcgt gatacatgtt
    22381 attcccttag gtatgtagac gggaaaacag taactggcat tatgaaaaaa ggccacctga
    22441 aaagtgtggc ctgatgggag tgagatgtaa acattgcggc agtttcagtc gacttgtatt
    22501 gccggatgcg gcgtaaacgc cttatccggc ctaccgccgc gatctgtagg ccggataaga
    22561 cgcgtcaagc gtcgcatccg gcaaataaca tctggctgat aattactgat tacccacctg
    22621 atcgccatac ggcagagtgt cgtaatcgat cagtgcgttt ttcttatacg ggttaccaat
    22681 ccagcgggta gtttctttga actgcggatt caccactgaa cgggttggat cgtaaccgtc
    22741 gtaagataag cccgccaaat ctgaccaggt gtggatcagt tccgccaggc tgtatttacg
    22801 atcaacatcc tgtgagaaat cacgcggatg agtcgcttgc catttttctg acgtccacag
    22861 caggaacgga atggtgtaca tatgacgcgt cgggttgtct tcattacgcc cctgagtttt
    22921 gtgcggcggc gtgtcgtaaa cctcttcacc gtggtcagag aaataaacca ggaaaccgtt
    22981 cggatcggtc gctttaaagt ctttaatcag gctggcaacc acgtgatcgt tgtacaggtt
    23041 agcattgtca taatcattat atgactccag ctcttctgcg tttaatcccg gtggaacatg
    23101 ctctgtatta ccgtcaaatt tgccctgatc ttccggataa cggtacttgt acttgatatg
    23161 cgtacccagc agatgaacga tgatcagttt cttcggcgca gggtctttca gcacttcctg
    23221 gaacggcttc agcacgttgg tgtcgtattc acgcgcactc tgcgtacgtt gctggttcat
    23281 gtagtactgc ttatcggtct ggcgcgaaaa taccgtcagc atggtattgc gggcggtcat
    23341 cgtctgctgg ttggtgatcc agaaggtttt ataacccgcc tgtttcatca tgttcatcag
    23401 cgacggctgc gtcagataca gatccgggtt cttttcattg gcaaaggtca gcgcctgttg
    23461 caggatttca atggtgtacg gacgagacgt aaccacgtta ttaaacacgg tcagattcgg
    23521 atcggtttta tgcagcgcat ccagctccgg cgtggtttca cgcggataac cgtacagact
    23581 catgcgtcca cgctgggtcg attcgccaat taccagcact aaggtgcgcg gttcgttacc
    23641 cgattcatct ttgaaattcg ccagcggcgg caaggcatta ttttcattca gtagctttgt
    23701 tagcgagttt agttgctgac gatactgata atagccggtc aggaattgcc acggtgcggc
    23761 aggctccata cgcgatgcca ggttatccaa cgttttctca aatggcttgt ttttgataaa
    23821 cgtattcatg gcgatcggat gcagaatcaa gccataaagt agggcaaaag agacaacata
    23881 acgccacggc tttggaatat agaccgggcg caggcgcgtc cacagcaaaa ctgccaccgc
    23941 cgtataggcc agcgcgataa gcacaatctt caggctgaaa tactggctta aatactcgct
    24001 ggcttcgttg gtgttggttt caaacatcac aaacagaacg ctctgcgaga actcctgacc
    24061 gtagatgacg tagtagcaca acgctgccag agaggccgcc catagcacca cgccgatcac
    24121 tgcggcaata attttaatcc gcttcggaaa gaggaatacc gggatcaacc acagcgaact
    24181 gaataacagc gagtcgcgaa tgccgttagt gccactataa ccactgatgt aaataatggc
    24241 ctgtagcaga gtagagaaaa accaaaagta gagcagtgcc caacccaggg ccttccagct
    24301 aaaaaggggt ttagcctgga cttctgtgga atgcatagta agaacctgtc ttgaaaaaaa
    24361 tatcgccgaa tgtaacgaca attccttaag gatatctgaa gggatatcca gattttgaat
    24421 aaaatgcaga cagaaatata ttgaaaacga gggtgttaga acagaagtat ttcagaaaac
    24481 cctcgcgcaa aagcacgagg gtttgcagga gaggaagatt agccggtatt acgcatacct
    24541 gccgcaatcc cggcaatagt gaccattaac gcctgctcga cgcgaggatc cggctcatgg
    24601 ccttcttttt ctgcctggcg ggagcggtgc agcaactcgg cctgcaatac gttcagcggg
    24661 tcggtgtaaa tattccgtag ctgaatagac tctgcaatcc acggcagatc ggccatcagg
    24721 tgagaatcgt tggcaatcgc cagcaccact ttgatgtctt cttcttgcag attgcgtaac
    24781 tctttaccta acggccacag tgctttgtct accaggcgtt ggtcatagta ttccgccagc
    24841 cacaggtctg ctttggcgaa gaccatctcc agcatgccga gacgcgtcga gaagaatggc
    24901 caatcgcggc acatggcttc cagctcgctc tgtttgccat cttcgaccac tttttgcagc
    24961 gccgtacctg cacccagcca ggcggggagc atcaggcggt tttgtgtcca ggcaaaaatc
    25021 cacggaatag cgcgcagtga ctcaacgcca ccggtcgggc gacgttttgc cggacgtgaa
    25081 cccagcggta gtttgccgag ttcctgctcc ggcgtggcag agcggaagta aggcacaaaa
    25141 tctttgtttt cacgcacgta gccgcggtag acatcgcatg agataaccga aagctcatcc
    25201 ataatgcgac gccagctctc tttcggctct ggcggtggca gcaggttggc ttccagaatc
    25261 gccccggtat acagcgaaag gctgctaacg gtgatttcag gcagaccgta cttgaagcgg
    25321 atcatctcgc cctgttcggt cacgcgcagg ccgcctttca ggcttcctgg tggttgtgac
    25381 agcagcgccg catgggcagg tgcgccgccg cgaccaatgg aaccgccgcg accgtggaac
    25441 aacgttagct caatacccgc tttttcgcag gttttgatta atgcatcctg tgcctgatat
    25501 tgcgcccagg aagctgccat cacgcccgca tcttttgctg agtcggaata gccaatcatc
    25561 accatctgtt tgccctgaat caggccgcga taccagtcga tattcagcag ctgggtcatg
    25621 acatcgttgg cgttgttcag gtcatcgagg gtttcaaaca gcggagcaac cggcatcgca
    25681 aacccgatac ccgcttcttt cagcagcagg tggacagcca gcacgtcgga cggcgttttc
    25741 gccatcgaga tcacgtaggc ggcaatggag ccttgcggtg cttcggcaat cacctggcag
    25801 gtatcgagca cttcgcgcgt ttcggcgctt ggttgccagt tgcgcggtag aagcggacgt
    25861 ttggagttca gttcgcggat caggaacgcc tgtttgtcgg cttctgacca gctctcgtag
    25921 tcgccgatac cgaggtagcg ggtcagctcg cccagcgctt cggtatggcg ggtgctctcc
    25981 tgacggatat caatacggac cagcggtacg ccgaaacatt tcacgcggcg cagggtgtcg
    26041 agcagatcgc cgttggcgat aatacccatg ccacacgcct gaagtgactg gtagcaagcg
    26101 tagagcggtt cccacagttc ttcgttttgt gtcagcaggc cttctggttt tggcagttct
    26161 tcgcctttca ggcgcgcttc cagccatgcc tgtgtcgcca tcaggcgaga acgcaggttt
    26221 ttcatcagat agcgatacgg ttctgcggca ccttcttcgc caaccagcgc cagcagttca
    26281 ggggtcgctt caaccatcga cagttcagaa accagcacct gaatatcttt caggaacaga
    26341 tcggtggctt tccagcgact gagcagcagg acgtggcggg tgatatcggc agtgacgttc
    26401 gggttgccgt cgcggtcacc gcccatccac gaggtaaaac ggaccggaac aaattcgacg
    26461 ggcagtttgt agccgagatt ctcttccagt tgttcgttca gttcgcgcag gtaattcggt
    26521 acgccttgcc acaggctgtt ttctactacg gcaaagcccc atttggcttc atctaccggg
    26581 cttggacgca gcttacggat ttcatctgta tgccatgact gggcgatcaa ctggcgcagg
    26641 cggcgcatca gctggttatg ttcgtagtcg gcgatatctt tgttatcgag ctgttttaag
    26701 caggcgttca cttccaccat tttgtggatc agtgtacgac gggtaatttc ggttgggtga
    26761 gccgtgagga ccagttccag cgacagcgat tccactgctt ttttgatggt gtcttcgctc
    26821 agttccggct ggtttttcag tttacgcagg gtgcgggcga tcacttccgg gttgctggca
    26881 gcttcgcctt tcggcgaaat gctgtggtat tgctcagcgg tgttggccag gttcaggaac
    26941 tgactaaacg cacgcgcaac gggcagcagc tcgtcgttcg acaaattttg taaggtggtg
    27001 agcaactcct ggcggttagc atcattgcca gcgcgtgaag atttcgacaa cttacggata
    27061 gtttctacgc gttcaagaat gtgttctccc aacgcatcct tgatggtttc tcccagcact
    27121 ttgccgagca tactgacatt actacgcaat gcggaatatt gttcgttcat attaccccag
    27181 acaccccatc ttatcgtttg atagccctgt atccttcacg tcgcattggc gcgaatatgc
    27241 tcgggctttg cttttcgtcg tcttttataa agccacgtaa aagcggtgac gtcaaatgct
    27301 gcgaaatcgc ttcagcaaac gaataaatag caggaattta cgccattaaa ttcatgacgc
    27361 tttaaataag cgtaacttat gggaatgtta aaaaatcgcc tcgagtaaca ccaaaggtgt
    27421 aggtcggata agatgcgtaa gcatcgcatc cgacattgtt tggcctacgt tttaatgcca
    27481 gcaaaaatgg tgaatcacct gggtgatcag ttcgcgggtg ggcttgataa acctcgtctc
    27541 cagatattca tcaggctgat gagcctgatt aattgagcca ggccccagaa ccagcgtcgg
    27601 gcataacgtt tgaataaacg gcgcttcggt acagtaattc accacttcgg tctttgctcc
    27661 gagcaatttc tcaaccactt caaccagttt atgattcggt gggcattcat agccagggat
    27721 cggcggatgc agctcgtcga ccgtcagacg acccggccag cgttcgctca ctggagccaa
    27781 tgcatcgttg agcaaaccat taagttcatt gagtgtcatg cctggcagcg gacgaatatc
    27841 catatgcaac tcacagcaag cacaaatacg gttagaagcg tcgccaccgt ggatatgccc
    27901 gaggttgagc gtagggtatg gcacggtaaa cgcgtcgtag tgataacgtt ctttcaggtt
    27961 atcgcgcaat tgcaaaatat gcccgatggc gtcgtgcatc agttcgatag cgttaactcc
    28021 gcgtgctgga tcgctggagt ggcccgactg gccctgaata cggatggcgt tagagatatg
    28081 acctttatgc gcgcgtaccg gttgcagcga cgttggctcg ccaatgatgg cgcaatccgg
    28141 gcgcagggcg gtagtttcgg caaaataacg cgctccggcc atactggttt cttcatcggc
    28201 agtcgccaga atgtagagcg gttttgcaag cttcgtgacg tcgacatcgc gtagcgcatc
    28261 aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct aagccgtaaa gcttgccgtc
    28321 atgctccgtc aacgtaaatg gatcgcgcgt ccagcgtccg tcatcaaatg gcaccgtatc
    28381 ggtatgcccc gccagcaaca aaccgccagc cccctgtccg caactggcca gcatattaaa
    28441 tttgttgcga gttcctggca caggctgtac ttccacattg aagcctaaat ccttaaacca
    28501 gtccgccagc agagtgatta aatctgcatt gctttgatcg agtgcctctt ccgtggcgct
    28561 tattgaaggt gtggcaatca gagcacggta aatctcgata aatggcggta atttgttttt
    28621 cattgttgac acacctctgg tcatgatagt atcaatattc atgcagtatt tatgaataaa
    28681 aatacactaa cgttgagcgt aataaaaccc accagccgta aggtgaatgt tttacgttta
    28741 acctggcaac cagacataag aaggtgaata gccccgatgt tgaatacgct gattgtgggt
    28801 gccagcggct acgctggcgc agagctagtg acctatgtaa atcgccatcc gcatatgaac
    28861 ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg gaaagttaat ctccgatttg
    28921 catccgcagc taaaaggcat cgttgatctg ccgttgcagc cgatgtcgga tatcagcgag
    28981 tttagcccag gggtggacgt ggtgtttctc gccaccgccc atgaagtcag ccacgattta
    29041 gcgccgcaat ttcttgaagc aggctgtgtg gtgttcgacc tttccggcgc gtttcgtgtt
    29101 aacgatgtcg ccttctatga aaaatattac ggctttaccc atcaataccc ggaactgctg
    29161 gaacaggcag cctacggtct ggcggagtgg tgcggtaata aattaaaaga agcgaacttg
    29221 attgccgtgc caggctgtta tccgacggca gcacagctgg cgctgaaacc gttgattgat
    29281 gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg ccaccagcgg cgtgagcggt
    29341 gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag ttagcctgca accgtatggc
    29401 gtctttaccc atcgccatca accagagatc gccacacacc tcggtgctga cgttatcttc
    29461 accccgcatc tgggcaattt cccgcgcggc attctcgaaa ccattacctg ccgcctgaaa
    29521 ccgggtgtga gccaggtgca agtggcgcaa gcgttacagc aggcgtatgc ccataaacca
    29581 ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa atgtcgttgg actgccattc
    29641 tgcgatatcg gttttgccgt tcagggcgag catctgatta ttgtggcgac cgaagacaac
    29701 ttactgaaag gcgcggcggc acaagcggtg cagtgcgcca atattcgttt cggctatgcg
    29761 gaaacgcagt ctcttattta agggtgcaat gatgaatcca ttaattatca aactgggcgg
    29821 cgtactgctg gatagtgagg aggcgctgga acgtctgttt agtgcactgg tgaattatcg
    29881 tgagtcacat cagcgtccgc tggtgattgt ccacggtggc ggctgcgtag tggatgagtt
    29941 gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaatggc ctgcgggtga cgcctgctga
    30001 tcagattgac attatcaccg gagcactggc gggaacggca aataaaaccc tgctggcatg
    30061 ggcgaagaaa catcagattg cggccgtagg tttgtttctc ggtgacggcg acagcgtcaa
    30121 agtgacccag cttgatgaag agttaggcca tgttggactg gcgcagccag gttcgcctaa
    30181 gcttatcaac tcattgctgg agaacggtta tctgccggtg gtcagctcca ttggcgtaac
    30241 agacgaaggg caactgatga acgtcaatgc cgatcaggcg gcaacggcgc tggcggcaac
    30301 gctgggcgcg gatctgattt tgctctccga cgtcagtggc attctcgacg gcaaagggca
    30361 acgtattgcc gaaatgaccg ccgcgaaagc agaacaactg attgagcagg gtattattac
    30421 tgacggcatg atagtgaaag tgaacgcggc gctggatgct gcgcgcacgt tgggccgtcc
    30481 ggtagatatc gcctcctggc gtcatgcgga acagcttccg gcactgttta acggtatgcc
    30541 gatgggtacg cggattttag cttaagtttt gttggccgga ggcgcagctt tccggcattg
    30601 aatttcaaaa taaggaaaca gagttatggc actttggggc gggcgtttta cccaggcagc
    30661 agatcaacgg ttcaaacaat tcaacgactc actgcgcttt gattaccgtc tggcggagca
    30721 ggatattgtt ggctctgtgg cctggtccaa agccctggta acggtcggcg tgttaaccgc
    30781 agaagagcag gcgcaactgg aagaggcgct gaacgtgctg ctggaagatg ttcgcgccag
    30841 gccacaacaa atccttgaaa gcgacgccga agatatccat agctgggtgg aaagcaaact
    30901 gatcgacaaa gtgggtcagt taggcaaaaa actgcatacc gggcgtagcc gtaatgatca
    30961 ggtagcaact gacctgaaac tgtggtgcaa agataccgtt agcgagttgc tgacggctaa
    31021 ccggcagtta caatccgctc tggtcgagac cgcgcagaac aatcaggacg cggtgatgcc
    31081 gggttacact cacctgcaac gcgcccagcc ggtgacgttc gctcactggt gcctggccta
    31141 cgttgagatg ctggcgcgtg atgaaagccg tttgcaggac gcgcttaagc gtctggatgt
    31201 cagcccgcta ggctgtggcg cactggcagg aacggcctat gaaatcgacc gtgaacagtt
    31261 agcaggctgg ctgggctttg cttcggcgac ccgtaacagt ctcgacagcg tttctgaccg
    31321 tgaccatgtg ctggaactgc tttctgctgc cgctatcggc atggtgcatc tgtcgcgttt
    31381 tgctgaagat ctgattttct ttaacaccgg cgaagcgggg tttgtggagc tttctgaccg
    31441 tgtgacttcc ggttcatcat taatgccgca gaagaaaaac ccggatgcgc tggagctgat
    31501 tcgcggtaaa tgcggtcggg tgcagggggc gttaaccggc atgatgatga cgctgaaagg
    31561 tttgccgctg gcttacaaca aagatatgca ggaagacaaa gaaggtctgt tcgacgcgct
    31621 cgatacctgg ctggactgcc tgcatatggc ggcgctggtg ctggacggca ttcaggtgaa
    31681 acgtccgcgt tgccaggaag cggcgcagca gggttacgcc aacgctactg aactggcgga
    31741 ttatctggtg gcgaaaggcg taccgttccg cgaggcgcac catattgtgg gtgaagctgt
    31801 ggtggaagcc attcgtcagg gcaaagcgtt ggaagatctg ccgctcagtg agttgcagaa
    31861 attcagccag gtgattggcg aagatgtcta tccgattctg tcgctgcaat cgtgcctcga
    31921 caagcgtgct gcaaaaggcg gcgtctcacc gcagcaggtg gcgcaggcga ttgcttttgc
    31981 gcaagctcgg ttagagtagt ttgtttatat tgctcccctg aaaatgggga gcttaccacg
    32041 cctacaaagt gcgacacttc gtaggcggat aaggcgttta cgccgcatca ggcagaaatt
    32101 tgtcagtatt ctggtgacct taaatttaac gcccaaccag aaccattatt tgtcacattc
    32161 ctttttattc ttgcgtcttc
//
LOCUS       DAZKJR010000047        31619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKJR010000047 DAZKJR010000000
VERSION     DAZKJR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.047"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..844
                     /locus_tag="ACHOWS_002180"
     CDS             17..844
                     /locus_tag="ACHOWS_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFQ0047746.1"
                     /translation="MLLIPCIGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDLYP
                     EYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGLNL
                     AFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNY
                     GAANENEWDGYRFKIKYSIPLTHLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNAIA
                     SSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQF"
     gene            complement(1061..3493)
                     /gene="metL"
                     /locus_tag="ACHOWS_002181"
     CDS             complement(1061..3493)
                     /gene="metL"
                     /locus_tag="ACHOWS_002181"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFQ0047747.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3496..4656)
                     /gene="metB"
                     /locus_tag="ACHOWS_002182"
     CDS             complement(3496..4656)
                     /gene="metB"
                     /locus_tag="ACHOWS_002182"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFQ0047748.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRVALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4933..5250
                     /gene="metJ"
                     /locus_tag="ACHOWS_002183"
     CDS             4933..5250
                     /gene="metJ"
                     /locus_tag="ACHOWS_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFQ0047749.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5310..5918
                     /gene="yiiX"
                     /locus_tag="ACHOWS_002184"
     CDS             5310..5918
                     /gene="yiiX"
                     /locus_tag="ACHOWS_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFQ0047750.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGELSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGKQQKLKEFDLSNPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5979..6191)
                     /gene="rpmE"
                     /locus_tag="ACHOWS_002185"
     CDS             complement(5979..6191)
                     /gene="rpmE"
                     /locus_tag="ACHOWS_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFQ0047751.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6394..8592
                     /gene="priA"
                     /locus_tag="ACHOWS_002186"
     CDS             6394..8592
                     /gene="priA"
                     /locus_tag="ACHOWS_002186"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0047752.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8748..9773
                     /gene="cytR"
                     /locus_tag="ACHOWS_002187"
     CDS             8748..9773
                     /gene="cytR"
                     /locus_tag="ACHOWS_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFQ0047753.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9865..10824
                     /gene="ftsN"
                     /locus_tag="ACHOWS_002188"
     CDS             9865..10824
                     /gene="ftsN"
                     /locus_tag="ACHOWS_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFQ0047754.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKIAGHTNCIRLAAGG"
     gene            10917..11447
                     /gene="hslV"
                     /locus_tag="ACHOWS_002189"
     CDS             10917..11447
                     /gene="hslV"
                     /locus_tag="ACHOWS_002189"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFQ0047755.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11457..12788
                     /gene="hslU"
                     /locus_tag="ACHOWS_002190"
     CDS             11457..12788
                     /gene="hslU"
                     /locus_tag="ACHOWS_002190"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFQ0047756.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            12855..13781
                     /gene="menA"
                     /locus_tag="ACHOWS_002191"
     CDS             12855..13781
                     /gene="menA"
                     /locus_tag="ACHOWS_002191"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0047757.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13874..14359
                     /gene="rraA"
                     /locus_tag="ACHOWS_002192"
     CDS             13874..14359
                     /gene="rraA"
                     /locus_tag="ACHOWS_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFQ0047758.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(14444..14689)
                     /gene="zapB"
                     /locus_tag="ACHOWS_002193"
     CDS             complement(14444..14689)
                     /gene="zapB"
                     /locus_tag="ACHOWS_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFQ0047759.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            15115..15960
                     /gene="glpF"
                     /locus_tag="ACHOWS_002194"
     CDS             15115..15960
                     /gene="glpF"
                     /locus_tag="ACHOWS_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFQ0047760.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15983..17491
                     /gene="glpK"
                     /locus_tag="ACHOWS_002195"
     CDS             15983..17491
                     /gene="glpK"
                     /locus_tag="ACHOWS_002195"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0047761.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            17662..18672
                     /gene="glpX"
                     /locus_tag="ACHOWS_002196"
     CDS             17662..18672
                     /gene="glpX"
                     /locus_tag="ACHOWS_002196"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFQ0047762.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            18769..19515
                     /gene="fpr"
                     /locus_tag="ACHOWS_002197"
     CDS             18769..19515
                     /gene="fpr"
                     /locus_tag="ACHOWS_002197"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFQ0047763.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(19520..19948)
                     /gene="uspD"
                     /locus_tag="ACHOWS_002198"
     CDS             complement(19520..19948)
                     /gene="uspD"
                     /locus_tag="ACHOWS_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFQ0047764.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(19975..20274)
                     /gene="yiiS"
                     /locus_tag="ACHOWS_002199"
     CDS             complement(19975..20274)
                     /gene="yiiS"
                     /locus_tag="ACHOWS_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFQ0047765.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(20486..20926)
                     /gene="yiiR"
                     /locus_tag="ACHOWS_002200"
     CDS             complement(20486..20926)
                     /gene="yiiR"
                     /locus_tag="ACHOWS_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFQ0047766.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            21027..21626
                     /gene="yiiQ"
                     /locus_tag="ACHOWS_002201"
     CDS             21027..21626
                     /gene="yiiQ"
                     /locus_tag="ACHOWS_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFQ0047767.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQALLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            21734..22501
                     /gene="tpiA"
                     /locus_tag="ACHOWS_002202"
     CDS             21734..22501
                     /gene="tpiA"
                     /locus_tag="ACHOWS_002202"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0047768.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(22556..23311)
                     /gene="cdh"
                     /locus_tag="ACHOWS_002203"
     CDS             complement(22556..23311)
                     /gene="cdh"
                     /locus_tag="ACHOWS_002203"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFQ0047769.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(23417..24406)
                     /gene="sbp"
                     /locus_tag="ACHOWS_002204"
     CDS             complement(23417..24406)
                     /gene="sbp"
                     /locus_tag="ACHOWS_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFQ0047770.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKVFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(24726..25688)
                     /gene="pfkA"
                     /locus_tag="ACHOWS_002205"
     CDS             complement(24726..25688)
                     /gene="pfkA"
                     /locus_tag="ACHOWS_002205"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0047771.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(25869..26771)
                     /gene="fieF"
                     /locus_tag="ACHOWS_002206"
     CDS             complement(25869..26771)
                     /gene="fieF"
                     /locus_tag="ACHOWS_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFQ0047772.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(26920..27420)
                     /gene="cpxP"
                     /locus_tag="ACHOWS_002207"
     CDS             complement(26920..27420)
                     /gene="cpxP"
                     /locus_tag="ACHOWS_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFQ0047773.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            27570..28268
                     /gene="cpxR"
                     /locus_tag="ACHOWS_002208"
     CDS             27570..28268
                     /gene="cpxR"
                     /locus_tag="ACHOWS_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFQ0047774.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            28265..29638
                     /gene="cpxA"
                     /locus_tag="ACHOWS_002209"
     CDS             28265..29638
                     /gene="cpxA"
                     /locus_tag="ACHOWS_002209"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFQ0047775.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(29744..30418)
                     /gene="yiiM"
                     /locus_tag="ACHOWS_002210"
     CDS             complement(29744..30418)
                     /gene="yiiM"
                     /locus_tag="ACHOWS_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFQ0047776.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGVSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(30567..31550)
                     /gene="kdgT"
                     /locus_tag="ACHOWS_002211"
     CDS             complement(30567..31550)
                     /gene="kdgT"
                     /locus_tag="ACHOWS_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFQ0047777.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT         7808 a         8321 c         8091 g         7399 t
ORIGIN      
        1 cgggatagct ttatccattc tattaatacc ttgcattgga aacgctcagg aaattaaaat
       61 tgatgaaagc tggttacatc aaagcttgaa tgtcattggt cgcacagact ctcgctttgg
      121 cccaagactg actaacgacc tctaccctga atatactgta gcaggaagaa aagactggtt
      181 tgatttttat ggttatgttg atctaccaaa attctttggc gtcggcagtc actatgatgt
      241 tgggatctgg gatgagggct caccactatt tacggaaata gaacctcggt tttccattga
      301 caaattgacc ggattaaatc ttgcgttcgg cccttttaaa gaatggttca ttgcaaacaa
      361 ctatgtctat gatatgggtg acaaccagtc atcccggcaa agtacatggt atatggggct
      421 tggtacagat atcgacacgg gtctaccaat taagctttct gccaatatat acgccaagta
      481 tcagtggcaa aactatggtg ccgctaatga aaatgaatgg gacggatatc gattcaaaat
      541 aaaatatagc attcctctta cccatttatt cggagggcga ttggtataca atagttttac
      601 taactttgat tttggctccg atcttgcgga caaatcacac aataataaac gaaccagtaa
      661 tgctattgct tcaagccata tcctttccct gctatatgaa cactggaaat ttgcatttac
      721 actacgttat tttcacaacg gtggacaatg gaatgctggg gagaaagtta acttcggaga
      781 tggtccattt gaattaaaaa acacaggatg gggaacctat actactattg gttatcaatt
      841 ttaaatcagg tgatgtttta gatattttta cttaatgcca ttttgtaatt gtcataaata
      901 acttcaccat tcacactgtg aattcatcgc tcaacacaaa agccccctta ttaccggggc
      961 actgaccaaa ggcaaaatga caatcaatca ccacctccgc aggaggtggt ttagaggtga
     1021 caacaaaagc tggcaactgc cagcttttgt tcaataatca ctacaacaac tgcgccagac
     1081 ggttaatatc cgactgaatc gccccggcgg tgacgtcgcg ccctgcacct ggcccgcgga
     1141 tcaccaacgg attatcgcga taccagcggc tttcgatggc aaagacgtta tcgcacggta
     1201 gtagtgatgc cagcggatgg tcttctcgca ccgcttccac gcctactcgc gctttgccgt
     1261 tggcatcgaa acgcgccacg tagcgcaaca ccagccccat ttcacgggcc gcttccaacc
     1321 gttgtaccat ctgctcatta agttcatcgc cattttcaaa gaaatggtcg atgcttccac
     1381 cttcgcaatg ggttggcacc agtgattcaa cacgcacctg gtccggttca atgttgtaac
     1441 ccgcttcacg cgccagaatc accagcttgc gcatcacatc tttgccggag aggtcatcac
     1501 gcgggtcagg ttcagttaag ccctgctgcc acgcctgatc caccagctca gtaaacggca
     1561 cgctaccgtc gaattgcagg aacagccagg agagcgtgcc ggagaagatc ccgctgatcg
     1621 acaaaatagt gtcgccgctg tcgatcagat cgcgcacggt atggttgatc ggcaaacctg
     1681 cgccgacggt ggcgttgtac agccagtgac gcccggtttt ttcgaacgcg tcgtggatct
     1741 ggcgatattt attgctgtcg ctcgctcccg ccagtttgtt ggcactgatg acgtggaaac
     1801 cgtggctggc gaaatcgaga tactgatcag caagctgctg gctggcagta acgtccagca
     1861 caactaaatc atcatacgga tgggcacgca tccacaggaa tagcgactct tcatcctgtt
     1921 cgacggcttc atcattaaag aaggctagcg cacggctggc gtccagcccg tcatagctca
     1981 acaggctgcg acggctgtcc accacgcctg ccagcacaaa ttcaaagccg gtgcgcgccg
     2041 agagcgtgct ctgctcacgg gcgaacagtt ccagccagcg ggaaccgata tttcccttac
     2101 cgaacaatac caggccgatg cgtttttctg cgcggaagac ggactgatgc agcccctgaa
     2161 tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag gctgatgccg tcatcggact
     2221 gccaggtaaa ctcgaccggc tggcctttca gttgctgcca gaagcggtgg caatgcagcg
     2281 ggttacgggt gacgcccgcc ccaaccatcg ccaccagcgc cagcccctga cgcagacgca
     2341 gttcgccggg taatcctgct tcgtcgagga ttttcagcgc actgtcggcc acttccgagg
     2401 tgtagcaaaa ttgcagcaac tggcgatcgt tatgcacgcc aactgccagt gggcgcacct
     2461 gagcacgttt caggatttgg tcgatctctt tatgcgccag tttaaaatcc tgactggcgg
     2521 gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt gacaatacgc gcaccagtac
     2581 cggaggccag cacgcgttca atgcgcgtgg aaccttgatc cggcgtgtag ctacagcgca
     2641 gctgcaggtc gatttcacta ccggatactg gctgtaaagt acgggcgtga agaacggggg
     2701 ccgccagacg cgccagttcg ctggcttcat caagacgcag caatggcagc agacaggcat
     2761 ctttcacttt acgcgggtcg gcactatata ccccggcgac atcgctccag atggttacgc
     2821 gggaaacacc cgccagcgca ccgatttgtg tcgcggaata gtcggaaccg ttacgcccca
     2881 gcagcaccgt ttcaccggcg ttgttacggc tgataaaccc ggtcaccacc agacgtttgc
     2941 ccggatgttg taccagtagc tgttgcagca acgggtaaga aagcccttca tcaacctgcg
     3001 gctgcgcggc gcgttcagcg cgtaaaaact cgcgggcatc aagccaggcc gctggcagcc
     3061 cttgttgatt aagtaccgca gacatcaggc gcgccgacca cacttcaccg tggcccacca
     3121 cttccgcata caccgcatca ttaataccgc tgtcgagcag cgccgcgagg cgttccagat
     3181 cgctgacaaa ggcgctaatg aggctatcgg cttcttcggc gggtagcagg ccgctaatca
     3241 gatcgcactg ataacgacgt agcgtttgtt gaacctgatg cgcagagaga cgatcggtct
     3301 ggcttagttt caaccagtta atcaactggt tagtggtgct accggcggcg gaaaccacca
     3361 tcatatcgtc aggctgagag tactccgcca taatgcccgc gacacgcaaa taacacttca
     3421 catcagccag actactgcca ccaaatttat gcagttgacg acctttcgcc cctgcctgcg
     3481 caatcacact catttttacc ccttgtttgc agcccggaag ccattttcca ggtcggcaat
     3541 taaatcttcg ccatcttcaa taccggtgga gatacgtagc agcgtctcgg agatcccggc
     3601 ggcagcacgc gcttctggcg ccatgcctgc atgtgtcatg gttgcggcgt gagagattaa
     3661 actttccact ccccctaatg attccgccag cgtaaacaac gacaacccgc ccaggaaacg
     3721 acgcagcgtc tgctcatcgc catccagttc aaaactcaac attgcgccaa agcctttttg
     3781 ctggcgcgcg gcaatttcat gcccctgatt ttccggcagc gacgggtgat acagtttttt
     3841 caccaacggc tgggtttgca gatatttcac aatcgcctgc gcgttgcgct gcgccagctc
     3901 catacgcggc accagcgttc gcaacccgcg cagcagcaga tagctgtcaa acgcgccgcc
     3961 cgtcacgcca atattgtttg cccaccaggc aagttcagtg acaacgtccg ggtctttagc
     4021 aatcactacg ccggccacca cgtctgagtg accgttcaga tatttcgtac atgaatgcaa
     4081 caccagatcg gcacctaatg ccagcggatt ttgtaatgcc gggcttaaga aggtgttatc
     4141 caccacgctc accgccccga cttcccttgc cagatggcag attttcgcaa tatccacgac
     4201 gcgtaacaat ggattacttg ggctttctac cagtaccagt ttgggttttt ctgccagcgc
     4261 tacccgtaat gcctgttcat cgccttgatc aacaaacaac acgcgatagc aaccgcgttt
     4321 cgccagactg tcgaacaggc gatagctgcc gccatagcag tcgtgcggcg caaccagcag
     4381 atcgcccggt ttcaaaaaga cggtcgttac cagatgaatc gcggacatgc cggtattagt
     4441 aagtacggca ccagcaccgc cttccagttc tgccagcgca cgctgaacca catcgcgcgt
     4501 tgggttgccg cgccgcgagt aatcatgcgc gcgcggttca ttaaatccgg taaagttata
     4561 ggtgctggaa agatggatcg gtgggacaac gcaaccatac tgttcgtcgt catttaaccc
     4621 gctacgcact gcgatggtgg cctgtttacg cgtcatgtga tgaagttccc tgggcttggt
     4681 cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg gacgtcaata catctggaca
     4741 tctaaacttc tttgcgtata gattgagcaa atcccaaata gccgttaaaa ttatatgcat
     4801 tatcacgccg acaggtgcat tacacgatgt cacggtagcg cctgtacggt aaactatgcg
     4861 ggtttacggt cagtacccac ataaattgtg tggtctggtc tcaatttatt gacgaagagg
     4921 attaagtatc tcatggctga atggagcggc gaatatatca gcccatacgc tgagcacggc
     4981 aagaagagtg aacaagtcaa aaagattacg gtttccattc ctcttaaggt gttaaaaatc
     5041 ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc gtcacgctac caacagcgag
     5101 ctgctgtgcg aagcgtttct gcatgccttt accgggcaac ctttgccgga tgatgccgac
     5161 ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa aagagatcat gcgtgagatg
     5221 gggattaacc cggagacgtg ggaatactaa agcggaaaag agcggcgcgg agttaaccag
     5281 ccgctttttt catacatgga cgtttaacga tgaaaatcag gctactgatc ctcagcctgc
     5341 tggtttctgt gcctgccttt gcctggcagc cgcaaactgg cgacatcatc tttcaaatct
     5401 ctcgctcatc gcaaagtaaa gcgatccaac tggcgaccca ttccgattac agccacaccg
     5461 gtatgctggt gatacgcaac aaaaagccct acgtttttga agcggtcggc ccggtaaaat
     5521 acaccccact caagcagtgg atcgcccatg gtgaaaaggg caaatacgtt gttcgccgcg
     5581 ttgaaggcga actgagtgtt gaacaacagc aaaaactggc gcaaacggca aaacgttatc
     5641 tcggtaaacc gtatgactcc agcttctcgt ggagcgacga tcgccagtac tgttcggaag
     5701 tggtttggaa ggtttatcag aacgcgctgg gaatgcgcgt gggcaaacag caaaagttga
     5761 aagagttcga ccttagtaac ccgcaggttc aggcaaagct caaagaacgc tacggcaaaa
     5821 atattccgct tgaggaaacg gtggtctcgc cacaagccgt tttcgatgcg ccacaactca
     5881 ccacggtcgc caaagaatgg ccgctgtttt cgtggtaata cgaccaaaat tcaggcacaa
     5941 aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg
     6001 aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg
     6061 aagaacgggt ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg
     6121 attttcatta cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata
     6181 tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag
     6241 acaccacgcg atgttaaaag tatagcttca atacgatcat ttcgtatgaa ggcgcgaaat
     6301 catacagaaa ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata
     6361 ctaacccacc gaatttcaag tcaggatgat gctatgcccg ttgcccacgt tgccttgccc
     6421 gttccgcttc cccgtacctt tgactatctg ctgccagaag gcatgacggt caaagccggg
     6481 tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca tcggggttgt ggtgtcagtt
     6541 agcaatgtca gcgagctgcc gcttaacgag ctgaaagcgg tagttgaagt gctggacgtt
     6601 gagccggtat ttactcactc cgtctggcga ttactgctct gggcggcaga ttactatcat
     6661 catccgattg gcgatgtgct gtttcatgcc ttgccgattt tactacgcca ggggcggcct
     6721 gcggcgaacg cgccgatgtg gtactggttt gccactgaac aaggtcaggc ggtggatctg
     6781 aacagcctga aacgctcacc caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa
     6841 atctggcgcg accaggttgc cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc
     6901 aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt ttagtgactg gcgaaagaat
     6961 tatgccgttt ctggtgagcg gttgcgattg aatactgaac aggccaccgc cgttggcgca
     7021 attcatagtg cggcggatac cttctctgcc tggctgctgg cgggcgttac cggttccggt
     7081 aaaacggagg tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg
     7141 gtgatggtgc cggaaatcgg cctgacgccg caaactatcg cccgttttcg tgaacgtttt
     7201 aatgcgccag tggaagttct gcattccggc ctgaacgaca gcgagcgtct ttcggcgtgg
     7261 ttgaaagcga aaaatggtga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg
     7321 ccgtttaaaa atctcggtgt gattgtcatt gatgaagagc acgacagttc ctacaagcag
     7381 caggaaggct ggcgctatca tgcccgcgac ctggcggttt atcgtgcgca cagcgagcaa
     7441 atcccgatta ttcttggctc cgcaacaccc gcactggaaa cgttatgcaa cgtccagcag
     7501 aaaaaatacc gcctgctgcg cctgacgcgt cgggcgggga atgcgcgtcc ggcaattcaa
     7561 cacgtgctgg atttaaaagg tcagaaggtg caggcggggc tggctccggc gttaatcacc
     7621 cgtatgcgcc agcatttaca ggccgacaac caggttatcc tctttcttaa ccgccgtggc
     7681 tttgcgcctg ccttgctgtg ccacgactgc ggctggattg ccgaatgtcc acgttgcgat
     7741 cactactaca cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagccaa
     7801 cgcccggtgc cgcgccagtg cccttcctgc ggttccacgc acctggtgcc ggtggggctg
     7861 ggcaccgaac agcttgaaca gacgcttgcg ccgctgttcc ctggtgtgcc catttctcgt
     7921 atcgaccgcg ataccaccag ccgcaaagga gcgctggaac aacaactggc ggaagtgcat
     7981 cgcggcggcg cgaggatctt gatcggcacg caaatgctgg cgaaaggtca ccactttccg
     8041 gatgtgacgc tggtggcgtt actggacgta gacggcgcgc tgttttctgc cgattttcgc
     8101 tcggcagagc gtttcgccca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt
     8161 aaacagggcg aagtggtgct gcaaacgcat catccagaac acccgctatt gcaaacgttg
     8221 ctctataaag gctatgacgc ctttgccgaa caggcactgg ctgagcgacg catgatgcag
     8281 ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcacgcg
     8341 ccgctgttcc tgcaacaact gcgtaatctc atcctctcca gcccgctggc agacgacaaa
     8401 ctgtgggttc tcggtcccgt tccggcactg gcacctaaac gtggcggccg ctggcgctgg
     8461 caaattttgt tgcagcaccc ttcccgcgtg cgattacagc acatcatcaa tggtacgctg
     8521 gcgcttatta atacaatacc ggattcccgt aaggtgaaat gggtgctgga tgtcgatccc
     8581 atcgagggct aatgcgctca cgatgcgagg cggatcgaaa aattcaatat tcatcacact
     8641 tttcatgaaa attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga
     8701 agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga
     8761 agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga
     8821 ctgtctcccg agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg
     8881 aaaaagcggc ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta
     8941 atgaatcccg cactattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa
     9001 ttattcgcgg tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact
     9061 gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg
     9121 atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta
     9181 atctgccgcc gatggtgatg gcgaacgaat ttgcaccgga actggagctg cctaccgttc
     9241 atatcgacaa tctgaccgcc gcatttgatg cagtaaatta tttatatgag caagggcata
     9301 atcggattgg ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag
     9361 gctatgtcca ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg
     9421 gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca gttgcttgat cttccacaac
     9481 cgcctactgc tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa
     9541 aacgccaggg gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc
     9601 tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc
     9661 gggaagctat gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc
     9721 gtttaatgga ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac
     9781 ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga
     9841 acgaataaat acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg
     9901 gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt
     9961 tctcccgcta tggtcgctat tgctgccgcc gttcttgtga cctttatcgg tggtctgtac
    10021 ttcattacgc atcacaagaa agaagagtcc gagacactgc aaagccagaa agtgaccgga
    10081 aacggactac caccaaaacc agaagaacgc tggcgctaca tcaaagagct ggaaagtcgc
    10141 cagccgggtg tgcgtgcgcc tacagaacct tctgccggtg gtgaagtgaa aacgccggag
    10201 caactgacac cagaacaacg ccagcttctt gagcaaatgc aggctgatat gcgccagcag
    10261 ccaacgcagc tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg
    10321 ctacagcgcc agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg
    10381 cgtacgactg aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag
    10441 gcgcaaccgc gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa
    10501 acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc
    10561 gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc
    10621 ggttcgttca gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaaggc
    10681 tttgactcaa aaattaccac caacaatggc tggaatcgtg tggtgattgg cccggtgaaa
    10741 ggcaaagaga acgcagacag caccctcaat cgcttgaaga tagcgggtca tacaaactgc
    10801 attcggctcg ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat
    10861 gccccgtact tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga
    10921 caactatagt aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca
    10981 cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca
    11041 aagtcatcgc gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg
    11101 aacgtaaact ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctggcgaaag
    11161 actggcgtac cgatcgcatg ctgcgcaaac ttgaagcatt gctggcagtc gcggatgaaa
    11221 ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg
    11281 ctatcggctc cggcggccct tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg
    11341 aacttagcgc ccgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct
    11401 ataccaacca tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt
    11461 ctgaaatgac cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca
    11521 acgccaagcg ttccgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg
    11581 aagagctgcg ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg
    11641 gtaaaactga aatcgcccgt cgcctggcta agctggcgaa tgcgccgttc atcaaagttg
    11701 aagcgaccaa attcaccgaa gtgggctatg tcggtaagga agtggattct attattcgcg
    11761 atctgaccga tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc
    11821 gcgctgaaga actggcagaa gaacgtattc tcgacgtact gatcccaccg gctaaaaata
    11881 actggggaca gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa
    11941 aactgcgtga aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga
    12001 tgggcgttga aattatggct cctccgggca tggaagagat gaccagccag ctgcagtcca
    12061 tgttccagaa cctgggcggt cagaaacaaa aagcgcgtaa gctgaaaatc aaagacgcca
    12121 tgaagctgct gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag
    12181 atgctatcga cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct
    12241 gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc
    12301 tgccgctggt agaaggttgc actgtttcca ccaaacatgg gatggtcaaa actgaccaca
    12361 ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc gtctgacctg atcccggaac
    12421 tgcaaggtcg tctgccaatc cgcgttgagc tgcaggcact gaccaccagc gacttcgagc
    12481 gtattctgac cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcaactg
    12541 aaggcgtaaa tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc
    12601 aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt acataccgtt ctggagcgtt
    12661 taatggaaga gatttcctac gacgccagcg atttaagcgg tcaaactatc actattgacg
    12721 cagattatgt gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta
    12781 tcctataatc gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt
    12841 tattggcgcg tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt
    12901 tacgacctaa aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat
    12961 ggtggcaagg tcacttcgat ccactggtcg ccctgctggc attgattacc gccgggctat
    13021 tacagatcct ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac
    13081 ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga
    13141 aacgggcgct cattattacc gttgtgctca tctgtctttc cgggctggca ctggttgcag
    13201 tggcgtgcca tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca
    13261 ttgccgctat cacctacacc gtgggcaatc gtccttatgg ttatatcggt ttgggtgata
    13321 tttccgtact ggttttcttt ggctggttga gcgtcatggg gagctggtat ttacaggctc
    13381 atacattgat tccggcactg atccttccgg caaccgcatg cggcctgctg gcaacggcag
    13441 tactgaacat taataacctg cgtgatatca atagcgaccg cgaaaacggc aaaaacacgc
    13501 tggtggtgcg cttaggtgca gtaaacgcgc gtcgttatca tgcctgcctg ctgatgggct
    13561 cactggtgtg tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt
    13621 tcctgctggc ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc
    13681 cggtggcgat gcgaccgatg ctggaacgca ctgtcaaggg agcgttactg actaacctgc
    13741 tgtttgtttt agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac
    13801 aattgatgat tttgccaaca gcctacatag cgcgatatac tgaaaattct cgcagcaact
    13861 gaatgttaag cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt
    13921 taacgtcgtg gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat
    13981 aatcacggta aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg
    14041 ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc
    14101 tgaactggcg cgtctggcag tacaaaatga atgggaaggt ctggtcattt acggcgcggt
    14161 gcgtcaggta gatgacctgg aagagctgga tatcggcatc caggcaatgg ccgcaattcc
    14221 ggttggtgcc gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt
    14281 aaccttcttc tccggcgacc atctttacgc cgacaatacc gggattattc tttcagaaga
    14341 tccgctggat attgaatgat aaaaaaggca ccgcaaggtg ccttttttct gcgttacctg
    14401 ttggcctaca cagtaaagaa attacgcgaa agatgaagcg taatcagacc tcttccatgc
    14461 gacccagcag ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt
    14521 tctcacgctc cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt
    14581 tgtttttttc tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct
    14641 gctgtacttt tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga
    14701 attgcaaggc gttgatggat aaaaaatcct cgtcccgatt accggtgacg ccttaataaa
    14761 tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca
    14821 cgccttgcag attacggttt gccacacttt tcatccttct cctggagaca taatccacac
    14881 caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc atcatgtaca
    14941 atcagatgga ataaatggcg cgataacgct cattttatga cgaagcacac acattttaag
    15001 ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt
    15061 ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt
    15121 caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt
    15181 tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg
    15241 gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt
    15301 tccggcgcgc atcttaaccc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac
    15361 aagcgcaaag ttatcccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct
    15421 ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca tcatattgtt
    15481 cgcggcagtg ttgaaagtgt tgatctggct ggcactttct ctacttaccc taatcctcat
    15541 atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg
    15601 atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg
    15661 attggtctac tgattgcggt cattggcgca tctatgggcc cattgacggg ttttgccatg
    15721 aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc
    15781 gcctttaccg gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt
    15841 ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt gccttgcgat
    15901 atctgtgttg tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa
    15961 tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg
    16021 caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca
    16081 gcgcgaattt gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat
    16141 ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga
    16201 tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga
    16261 aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg
    16321 cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg gtctggtgat
    16381 tgacccgtac ttctctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg
    16441 cgagcgtgcg cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa
    16501 aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt
    16561 caacatccat accctggact gggacgacaa aatgctggaa gtgctggata ttccgcgcga
    16621 gatgctgcca gaagtgcgtc gttcttccga agtgtacggt cagactaaca ttggcggcaa
    16681 aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggcgg cgctgtttgg
    16741 tcagttgtgc gtgaaagaag gaatggcgaa gaacacctat ggcactggct gctttatgct
    16801 gatgaacact ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg
    16861 cggcccgact ggcgaagtaa actatgcgct ggaaggtgcg gtgtttatgg cgggcgcgtc
    16921 cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt
    16981 cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggccttta ccgggctggg
    17041 tgcgccatac tgggatccgt atgcacgcgg ggcgattttc ggtctgactc gtggggtgaa
    17101 cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct
    17161 ggaagcaatg caggccgact ccggtattcg tctgcacgcg ctgcgcgtgg acggcggtgc
    17221 ggtggcgaac aatttcctga tgcagttcca gtccgatatt ctcggtaccc gcgttgagcg
    17281 cccggaagtg cgcgaagtca ccgctttggg tgcggcctat cttgctggtc tggcggttgg
    17341 cttctggcag aatcttgacg agctgcaaga gaaagcggtg attgaacgcg agttccgtcc
    17401 aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag cggtcaaacg
    17461 cgcgatggca tgggaagaac acgacgagta ataatgattg cctgatgcga cgcttgcgca
    17521 tcttatcagg cctacgagtc ggatacacat ccggcatcac gcagactctg aaaccgtgcg
    17581 ggggcaaccc cgcacacatc aataatcccg cccttcccct gtgctacact tcgcgccatt
    17641 ccttactgct tagagtttgc tatgagacga gaacttgcca tcgaattttc ccgcgtcacc
    17701 gaatcagcgg cgctggctgg ctacaaatgg ttaggacgcg gcgataaaaa caccgcggac
    17761 ggcgcggcgg taaacgccat gcgtattatg ctcaaccagg tcaacattga cggcaccatc
    17821 gtcattggtg aaggtgaaat cgacgaagca ccgatgctct acattggtga aaaagtcggt
    17881 actggtcgcg gcgacgcggt agatattgct gttgatccga ttgaaggcac gcgcatgacg
    17941 gcgatgggcc aggctaacgc gctggcggtg ctggcagtag gcgataaagg ctgtttcctc
    18001 aacgcgccgg atatgtatat ggagaagctg atcgtcggac cgggagccaa aggcaccatt
    18061 gatctgaacc tgccgctggc ggataacctg cgcaatgttg cggcggcgct cggcaaaccg
    18121 ttgagcgaac tgacggtaac gattctggca aaaccgcgcc acgatgccgt tatcgcagaa
    18181 atgcagcaac tcggcgtgcg cgtatttgct attccggacg gcgacgttgc agcctcaatt
    18241 ctcacctgta tgccagacag cgaagttgac gtgctgtacg gtattggtgg cgcgccggaa
    18301 ggcgtggttt ctgcggcggt gatccgcgca ttagatggtg acatgaacgg tcgtctgctg
    18361 gcgcgtcatg acgtcaaagg cgacaacgaa gagaatcgtc gcattggcga gcaggagctg
    18421 gcacgctgca aagcgatggg catcgaagcc ggtaaagtat tgcgtttggg cgatatggcg
    18481 cgcagcgaca acgtcatctt ttctgccacc ggcatcacca aaggtgatct gctggaaggt
    18541 attagccgca aaggcaatat cgccaccacc gaaacgctgc tgatccgtgg taaatcacgc
    18601 accattcgtc gcattcagtc catccactat ctggatcgta aagacccgga aatgcaggtg
    18661 cacatcctct gattgatttg atcgattgag ccttccagtc cttcgggact ggaatttttt
    18721 tgttcggaga acgaagataa ggcaagtcaa tcaaaacagg agaaaaacat ggctgattgg
    18781 gtaacaggca aagtcactaa agtgcagaac tggaccgacg ccctgtttag tctcaccgtt
    18841 cacgctcccg tgcttccgtt taccgccggg caattcacca aacttggcct tgaaattgaa
    18901 ggcgaacgcg ttcagcgcgc ctactcctat gttaactcgc ccgataatcc ggatctggag
    18961 ttttacctgg tcaccgtccc cgatggcaaa ttaagtccac gactggcggc actgaaaccg
    19021 ggcgatgaag tgcaggtggt tagcgaagcc gctggcttct ttgtgctgga tgaagtacca
    19081 gattgcgaaa cgctatggat gctggcaacc ggtacagcga ttggccctta tttatcgatt
    19141 ctgcaattgg gcaaagattt agaacgcttc aaaaacatgg tgctggttca cgccgcacga
    19201 tatgccgccg acttaagcta tctacctctg atgcaggaac tggaaaaacg ctacgagggg
    19261 aaactgcgca ttcagacggt ggtcagtcgg gaaacggctg cgggttcgct cactgggcga
    19321 ttaccggcgt taattgaaag tggtgaactg gaaagcgcgg ttggcctgcc gatgaataaa
    19381 gaaaccagcc atgtgatgct gtgcggcaat ccacagatgg tgcgcgatac acaacagttg
    19441 ctgaaggaga cccggcagat gacgaaacat ttacgtcgcc gaccgggcca tatgacagcg
    19501 gagcattact ggtaagcggt tacttgtcga taaacggcac gatcaagaga tccgcactca
    19561 acttattgat catcccgcga tatgccggca tcaaacggtt gataaatgag tgatgatgac
    19621 cacagacaag gaggtcgcac tgctcttttt gcataatttc cagcagcgtt tccggcattt
    19681 ctccgcgttc aatacgcagt tttgtcttcg gccattgaat atttttcgtc agtttataca
    19741 gcttgttatc cgacttattc ttcaacaatt gaagaatatc ttctgttgca gggaagtaga
    19801 tacccggata caactcgctt aagccatcat caatatgaat taacgtcagg tgagcgtcat
    19861 tatgtctggc gagctccagg gctttattca ccagtaaggc atcttcttca ttcccggaaa
    19921 ttgccacgcc aatgtgttta taagccatgt ttaactcctt ctaaagcaac tccatcaagc
    19981 taacgaacgc agtgataact caaaaataat catctcggcc tggcatgaga aggtgaaggc
    20041 cgcatcaagc tcaacttgcc cctccagatc cttaatctta taggccacgc tggccccttc
    20101 atctgcagag gatgcagcgg cggcaagctc tttcagtttg gtcataaaag actctgcttc
    20161 ttcgcgggtg gcaaaaaagc gcgaaaactt actggtacag ttgtcgttat caatcaccgt
    20221 accgatatca atcgcacatc ctttagtact gcatttatct acgacatctt tcatagggca
    20281 cctctgtata ttcgcctctc tgtcgattac gacttaagaa taatgcgccg ttaaaggaag
    20341 aacaaacaag taatgatctt atggattacc attttttttg atattgctta aggtgaagag
    20401 ttcagaagga ttttccgggc attgtcactt gcatcgtgga aataacaatc ccggaaaaaa
    20461 atagcgataa gagatatatt aaaggttaat tacttgattt attgtcggct ttatacttca
    20521 catcctgggt atctttacca tatttatttt cgccttgtgt gccaacaaac gcaccaagat
    20581 cgataagcat catcaccaga atcaatgtcg ggacaaaacg ccccaccgcc cattgccaga
    20641 cacccggtaa aattgcccag ttacccgcca gcagcatcca cgccacaatc atcagaaatg
    20701 cccatgcgcc ggaacgcccg cgatcatgca agcgcttaac ggttactgcc gctgttggcc
    20761 aaagcaagca cacaaggcaa aacgccgcgg tctgaatatc gagtaaattt ttacccgcca
    20821 gtgagaaaag caccagcatg cctgcgaacc acaggcctat ccaaatccag aaatcacggc
    20881 gtccaatacg ccctttaaat gagaataacc attgctgtat ggtcatgtaa gttccttgat
    20941 ggttgtcttt tccaggattc tacccttttg acaagggcga cagatatcgt tttaatcgga
    21001 gccagtcata acaaaaggta ctgtcaatga agccagggtg tacgctgttt tttctcttat
    21061 gttctgcatt aaccgttaca acaacggcgc atgcacaaac accagatacg gcaacgaccg
    21121 cgccttatct gctggctgga gcccctactt tcgatctctc catcagccag tttcgagaag
    21181 actttaacag ccagaatccc agcctgccac tgaacgaatt tcgtgccatc gacagcagtc
    21241 ccgacaaagc caatctcact cgtgcagcaa gtaaaattaa cgagaacttg tatgcttcta
    21301 ctgcgctgga gcgcggtacc ttaaaaatca aaagcattca aatgacctgg ctacccatcc
    21361 aggggccaga gcaaaaagcc gcgaaagcga aagctcaggc atacatggca gcggtgatcc
    21421 gcacactcac cccattaatg accaaaactc aaagccagaa aaaactgcag gcgctactaa
    21481 cggcggggaa aaacaaacgt tattacaccg agacagaagg tgcactgcgt tatgttgtcg
    21541 cggacaacgg cgaaaagggg ctgaccttcg ctgttgaacc gattaagctg gcgctatctg
    21601 aatcgcttga aggtttgaat aaatgacaaa aagcaaagcc tttgtgccga tgaatctcta
    21661 tactgtttca cagacctgct gccctgcggg gcggccatct tcctttattc gcttataagc
    21721 gtggagaatt aaaatgcgac atcctttagt gatgggtaac tggaaactga acggcagccg
    21781 ccacatggtt cacgagctgg tatctaacct gcgtaaagag ctggcaggtg ttgctggctg
    21841 tgcggttgca atcgcaccac cggaaatgta catcgatatg gcgaagcgtg aagctgaagg
    21901 cagccacatc atgctgggtg cgcaaaacgt ggacctgaac ctgtccggcg cattcaccgg
    21961 tgaaacctct gctgctatgc tgaaagacat cggcgcacag tacatcatca tcggccactc
    22021 tgaacgtcgt acttaccaca aagagtctga cgaactgatc gcgaaaaaat tcgcggtgct
    22081 gaaagagcag ggtctgactc cggttctgtg catcggtgaa accgaagctg aaaacgaagc
    22141 gggcaaaact gaagaagttt gtgcacgtca gatcgacgcg gtactgaaaa ctcagggtgc
    22201 tgcggcattc gaaggtgcgg ttatcgctta cgaacccgta tgggcaatcg gtactggcaa
    22261 atctgcaact ccggctcagg cacaggctgt tcacaaattc atccgtgacc acatcgctaa
    22321 agttgacgct aacatcgctg aacaagtgat cattcagtac ggcggctctg taaacgcgtc
    22381 taacgctgca gaactgtttg ctcaaccaga catcgacggc gcgctggttg gcggtgcttc
    22441 tctgaaagct gacgctttcg cagtgatcgt taaagctgca gaagcggcta aacaggctta
    22501 agtctgacag gtgccggatt tcatatccgg cacttacttt ccttaactcc tcgccttaac
    22561 gcaaaatctc acactgatga tcctggattt cctcggctga agcacggtta agcgtcagta
    22621 gatttcgttg tgtcgccagc aatacaaatg agttatcact ctgccgcacc atcgccagcc
    22681 cgtagcttcc catatgttcc cgcgcctcag gcacttcttc tgcaagcatc ataaatgggc
    22741 tgcgttgtac cagttcgctt tccgttaccc gacgcgccag gtattcatgc ccgcgcaaac
    22801 cacctggcag tggcaaccag cggctgctga tgttcgccag attgttatcc agctgttcgc
    22861 gcacatcagg acgaatacaa gagatatgaa tatgaaaatg gttttgcgta cgcccggtgc
    22921 gagagttgat cgccaaagaa accgcgcgat cgggaaccgg ctggccgtat tttttgctca
    22981 taaaatcacg cgcctgccag gccaaccaaa agaagttcgg cgttgaagga tcggtcaaca
    23041 aaggactttc agtaccgtta atacgatacg ttggcatcaa cagatattgc agtgggccat
    23101 taagatcttt taaaaccacg tatccggcat tgggtttgac ttccgcacat ggcgaaggat
    23161 tttgattttg ctgctgattg ggcaaacatt cctcaaggac aatcttacgt aatgtatccg
    23221 actcttcacc ggttaatttc cagtaaccaa taccggcagc cacaacggcg ataactatca
    23281 tcaccaaaaa aagaagaccc gcttttttca tcttttttcc ctgtacctca aagagatgca
    23341 agggtaacgc aaaatcgtga caaataaaaa acccagtcgc gagaaaccac cgggtttgat
    23401 aattatcctg gagaaatcag cgtttgctaa tctgatcgaa cgtaccgccg ttagcaaaat
    23461 gctctttttg cgctttcgtc cagccgccga actcttcatc aatggtgaat aacttcagct
    23521 ttggaaacgc attttcgtac tttttcgcca cctcagcatc gcgcggacgg tagtagtttt
    23581 tcgcggcaat ttcctgacct tctggcgagt agagatattt caggtaggct tccgccacct
    23641 ctttggtgcc ttttttctcg accactttat cgaccaccga cacggttggc tctgcgagga
    23701 tagactcact cggcgtgaca atttcgaatt tatctttccc cagttcattc gctgccagca
    23761 gagcttcgtt ttcccaggca atcagtacat cgccaatccc gcgctcgaca aaggtattag
    23821 tggagccgcg cgcgccagaa tccagaactt cgacgttttt atacagtgcc cgaacaaaat
    23881 cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc gtagccccag gctgccaggt
    23941 agttccagcg cgcgccacca gagcttttcg gattaggcgt gatcaccgaa acacccggtt
    24001 taatcagatc gttccagtca tggatctgct tcggattgcc cttacgcacc aggaaaacaa
    24061 tggtggaagt gtacggtgcg gagttatccg gcagacgttt gatccactct ttatcaatcc
    24121 gcccgcgttc cgcaatcgcg tccacgtcat aggccagagc cagcgtgaca acatcagctt
    24181 caataccgtt gattaccgac gtcgcttgtt tgcctgaacc accgtgggac tgacggatca
    24241 ccacgttatc gccagtttgc tgtttccagt gggcgctgaa taccttgttg tactgttcgt
    24301 acaattcgcg cgttggatca tatgaaacgt taagaagctg aatatccttt gccataacgc
    24361 tggttgccgc cagcaaaaat gttaacccta cgccccactt gttcatcgcc cgactctctt
    24421 atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac caaacattaa agaataaaaa
    24481 aagattggct ataacttgcg ggtatatgta gggggattaa aaaggcggaa gaaatccgcc
    24541 tcatattgct gacaaagtgc gctttgtcca tgccggatgc ggcgcgaacg ccttatccgg
    24601 cctacaaaag ttcgcaaatt caataaattg cagaattcat gtaggcctga taagcgaagc
    24661 gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa aggaatctgc ctttttccga
    24721 aatcattaat acagtttttt cgcgcagtcc agccagtcgc ctttgaacgg acgcttcatg
    24781 ttttcgatag cgtcgatgat gtcgtggtga accagctgtt cgttctggat acctacgcaa
    24841 cgaccaccgt aacctgccag cagcagctcg atagcgtaag cgcccatacg ggaagccaga
    24901 atacggtcgt aaggcaccgg agaaccaccg cgctggatgt ggcccagcac agttgcgcgg
    24961 gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt
    25021 tcggtaatcg ccactatcgc gtgtttttta cctttcgcga tacccgcttt gatttcgttt
    25081 accaggtctt cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca gccaccggca
    25141 atagccgcag ccaaagtcag atcgccacaa taacggccca tcacttccac cacggaaata
    25201 cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc
    25261 gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc
    25321 agaccgatgc acgggaagcc catttcggtc agacgcattg cacccatgta ggaaccgtca
    25381 ccgccgataa ccaccagcgc gtcgatacca cgttttttca ggttttcgat agccacggcg
    25441 cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac cgaggaacgt accaccacgg
    25501 ttgatcatgt cagaaacgct gtaacggtct agctgtacca tacggtcttc atacagaccc
    25561 agatagccgt cataaatgcc cattacttcc agaccttctg tcagcgcaga acgaacaacc
    25621 ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac accgattttc
    25681 ttaatcatga ctacctctga actttggaat gcaaaatgaa atctgttgcc ggaagtcttc
    25741 ttgcacatcg aagtgatcca acgaatgtgc aaatagtata acaatcactt cctgctgaat
    25801 tgattcaggt caggccaaat ggcggtattt tatacacaaa atgcgggtct ggctctcttt
    25861 tatactgatt atgaaagcat agaccgttta ccctccctgg gtacgacgga acaggggtcc
    25921 tgatggataa tcacatccga tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc
    25981 gccaccatat gtgcctgaac caaaggcaga gagtcttcca tttccaaatg aatctgaata
    26041 aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag cgccgctaac acccggccag
    26101 gaagtcacga tatcaataat ttcttgccgt tcttcatcag gcaatgcgcg atccagtaat
    26161 gactgtaccg cctcatatcc catgcgtaac gcgctatata aaatatagat gccgattccc
    26221 aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg acaaccccag cgccagcaga
    26281 attgcaccgt tcatcataac atcagactgg taatgtagca tatcagcccg caccgcctgg
    26341 ctttgcgtcc ggcgcaccac ccaacgctga aacgagacaa ggataatcgt acaaattagc
    26401 gccacaattg tcacgataac cccgacgcct ggatctgtca tcggtgttgg agatatcaga
    26461 tgttgaatac ccgtcaaaaa caggaatagt gctgaaccgg agataaacat actttgcgcc
    26521 agcgccgcga gggactcagc tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc
    26581 agggaatatc gcaccaccag taaattcgtc aacgacgcgc cgatatccac cagcgaatcc
    26641 accagcgcgg cgagaatact caccgacccg gtataccacc atgcaaaaat tttaatcagc
    26701 aatagcagtg aagccatcgc cgttgcagca atcgccgccc gactgaccag ccgtccataa
    26761 gattgattca taaatactcc cgctatcaac tgacgctagt ataacggaag caaatcatct
    26821 gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa cccccacatc atgtggggga
    26881 agacagggat ggtgtctatg gcaaggaaaa cagggtttac tactgggaac gtgagttgct
    26941 actactcaat agcttcaacg atgaactttt ttgccattgc gtcacgtcac gcaactgctc
    27001 cattcgttgt tgatgtttct catttaaaac cgcttgctgc tccggcgtta acaggcgata
    27061 catttggttg cggacttttg ccatctcaac ctgacgagca atttgctcat tcgccatttt
    27121 ttctgcctgt gcgcgcacag cgttttcatc aaaattttct gcggtgacaa ggcgatgcat
    27181 tgtctccagt tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt gcataagatc
    27241 tcgcatctgc tgacgctgat gttcggttaa acttatgccg tcgaacatat ggctctgcgt
    27301 actgcgctgc gtaagttctt cacccggatg ccagttatcg cctgaaccga cttcagcagc
    27361 gtggcttaat gaactgactg ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat
    27421 catttgctcc caaaatcttt ctgtcgcgat tcaacgatag agagtttacg attcaggctg
    27481 caaacatgcg tcagggggtg taaaacaacg taaagtcatg gattagcgac gtctgatgac
    27541 gtaatttctg cctcggaggt atttaaacaa tgaataaaat cctgttagtt gatgatgacc
    27601 gagagctgac ttccctatta aaggagctgc tcgagatgga aggcttcaac gtgattgttg
    27661 cccacgatgg ggaacaggcg cttgatcttc tggacgacag cattgattta cttttgcttg
    27721 atgtaatgat gccgaagaaa aatggtatcg acacattaaa agcacttcgc cagacacacc
    27781 agacgcctgt cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg
    27841 agctgggcgc agatgactat ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta
    27901 ttcgcgcgat cctgcgccgt tcgcactgga gcgaacaaca gcaaaacaac gacaacggtt
    27961 caccgacact ggaagttgat gccttaagtc tcaatcctgg tcgccaggaa gccaccttcg
    28021 acgggcaaac actggagtta accggtactg agtttaccct gctctatttg ctggcacagc
    28081 atctgggtca ggtggtttcc cgtgagcatt taagccagga agtgctgggc aagcgcctga
    28141 cgcctttcga ccgcgctatc gatatgcata tttccaacct gcgtcgtaaa ctgccggatc
    28201 gtaaggatgg tcacccgtgg tttaaaaccc tgcgtggtcg tggctatctg atggtttctg
    28261 cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga cgctggcgct
    28321 ggtgttgatg ttggttttga tgttacccaa gctcgattca cgccagatga ccgagcttct
    28381 ggatagcgaa cagcgtcagg gtctgatgat tgagcagcat gttgaagcgg agctggcgaa
    28441 cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc
    28501 accgccagga cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg
    28561 cagcgaaatg cagatcattc gtaactttat tggtcaggcc gataacgccg atcatccgca
    28621 gaagaaaaag tatggccgcg tggaactggt cggtccgttc tccgtgcgtg atggcgaaga
    28681 taattaccaa ctttatctga ttcgtccggc cagcagttct caatccgatt tcataaactt
    28741 actgtttgac cgcccgctat tactgctgat tgtcaccatg ctggtcagta cgccgctgct
    28801 gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga
    28861 agttgcccaa ggaaacttac gccagcatcc ggaactggaa gcggggccac aggaattcct
    28921 tgccgccggt gccagtttta accagatggt caccgcgctg gagcgcatga tgacctctca
    28981 gcaacgtcta ctttctgata tctctcacga actgcgcacc ccgctgacgc gtctgcaact
    29041 gggtacggcg ttactgcgcc gtcgtagcgg tgaaagcaag gaactggagc gtattgaaac
    29101 cgaagcgcaa cgtctggaca gcatgatcaa cgatctgttg gtgatgtcac gtaatcagca
    29161 gaaaaatgcg ctggttagcg agaccatcaa agccaaccag ttgtggagtg aagtgctgga
    29221 taacgcggcg ttcgaagccg agcagatggg caagtcgttg acggttaact tcccgcctgg
    29281 gccgtggccg ctgtacggca acccgaacgc cctggagagt gcgctggaaa acattgttcg
    29341 taatgccctg cgttattccc atacgaagat tgaagtgggc tttgcggtag ataaagacgg
    29401 tatcaccatt acggtggacg acgatggtcc tggcgttagc ccggaagatc gcgaacagat
    29461 tttccgtccg ttctatcgta ccgatgaagc acgcgatcgt gaatctggcg gtacaggttt
    29521 ggggctggcg attgtagaaa ccgccattca gcagcatcgt ggctgggtga aggcagaaga
    29581 cagcccgctg ggcggtttac ggctggtgat ttggttgccg ctgtataagc ggagttaaac
    29641 tccgcatttg taggcaggat aaggcgttta cgccgcatcc ggcatttgag caggatgcct
    29701 gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg ggattatttt ccccacaatc
    29761 ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtt cgcgtccagc
    29821 ttttcgatac ccctgcggcg gagagtaaac ggtgatactg atcgtcatca aacggcatat
    29881 gccatgcgat ggcggcagcc tcctgcacgg tcacatcgct gacacgggaa accaactcca
    29941 gtggcgcatc agcagatacc aaccctggtg caattacgct atacaaccac cccactttac
    30001 ccgtgttttg catcaactgc gctatatcgc tgatatcaaa atggtaattg agtttatagc
    30061 aaggtgagcg tggctggctg acctgaatta atgcctctcc ccagcggaaa atatcgccaa
    30121 tataaacatt actttccgtc agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa
    30181 caaacaactc cgcctgttcc ggaaattccc gcgcccagta aagataatgc tcacgaggat
    30241 aatgacacag cgctctgtct ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt
    30301 ccagccccag gtccgtcagc atcaactcac catcaacctg gattttggca attgcgctgg
    30361 gtttgccttc gggataagcc tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct
    30421 gttccctctc cacgagaaat ttccccatta gaccatattt attttatgtc aggcgtgctg
    30481 ttttcgcggt aagtatccag gcaggaactc aatagcaaac cggacgaacg ttagttcatc
    30541 cggctgtggg gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct
    30601 gctctggctt tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc
    30661 acaatgaccg ccgtcgcgac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc
    30721 accatttctg caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca
    30781 attccggcag tgccatcgcc accgccaatc aatttatcag cgataatcaa cggaataccg
    30841 gtcacgataa ttactgccac acccaacagg atccccagca aacctgtctg ggcaattacc
    30901 gtcaaatcaa tggtattacc cagtgcgaag gcaaagaatg gaatcagcgt ctgcaccgct
    30961 ttgctgaaaa attctcgtaa ttcagggtca aggttcccaa gggcaaagcc caccaggaac
    31021 ggtaatactg cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga
    31081 ataatcatcg tcatgagcgg cccggactcc aatgacatca acacaaatgc cccagcttct
    31141 tcttttgtgc cgtactgctg catgatggaa gcgtaaagtc cgccgttggt catatccatc
    31201 gccgccacca gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc
    31261 ggaataatgc gcgaggcaat tgccgcaacc acccacgcga cggcaatttt tgttaccacc
    31321 agcgtaccgg atttacgcaa taccgttccc gtcgcgctta attttattga cgcccccatg
    31381 caaaaaaacc acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat
    31441 ccaaaatatt tccccgcctc cggcgagaag gtgtggcaca gtgcgccaag gaataacggg
    31501 acgagcatca tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc
    31561 tgtcggcata tttttaaggg ctgatgcgtc attcctcaat ggagcatcag tgttgatgg
//
LOCUS       DAZKJR010000048        31413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKJR010000048 DAZKJR010000000
VERSION     DAZKJR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.048"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..791)
                     /locus_tag="ACHOWS_002212"
     CDS             complement(<1..791)
                     /locus_tag="ACHOWS_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFQ0047778.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLVVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVP"
     gene            complement(968..1879)
                     /gene="gsiD"
                     /locus_tag="ACHOWS_002213"
     CDS             complement(968..1879)
                     /gene="gsiD"
                     /locus_tag="ACHOWS_002213"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFQ0047779.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1882..2802)
                     /gene="gsiC"
                     /locus_tag="ACHOWS_002214"
     CDS             complement(1882..2802)
                     /gene="gsiC"
                     /locus_tag="ACHOWS_002214"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFQ0047780.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVGDEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2820..4358)
                     /gene="gsiB"
                     /locus_tag="ACHOWS_002215"
     CDS             complement(2820..4358)
                     /gene="gsiB"
                     /locus_tag="ACHOWS_002215"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFQ0047781.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4378..6249)
                     /gene="gsiA"
                     /locus_tag="ACHOWS_002216"
     CDS             complement(4378..6249)
                     /gene="gsiA"
                     /locus_tag="ACHOWS_002216"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFQ0047782.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYRGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVVAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6236..7201)
                     /gene="iaaA"
                     /locus_tag="ACHOWS_002217"
     CDS             complement(6236..7201)
                     /gene="iaaA"
                     /locus_tag="ACHOWS_002217"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0047783.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     AMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7405..8640
                     /gene="moeA"
                     /locus_tag="ACHOWS_002218"
     CDS             7405..8640
                     /gene="moeA"
                     /locus_tag="ACHOWS_002218"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFQ0047784.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8640..9389
                     /gene="moeB"
                     /locus_tag="ACHOWS_002219"
     CDS             8640..9389
                     /gene="moeB"
                     /locus_tag="ACHOWS_002219"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFQ0047785.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYHCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCGVCGQ"
     gene            complement(9465..10127)
                     /gene="fsa"
                     /locus_tag="ACHOWS_002220"
     CDS             complement(9465..10127)
                     /gene="fsa"
                     /locus_tag="ACHOWS_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0047786.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10258..11157
                     /gene="ybiY"
                     /locus_tag="ACHOWS_002221"
     CDS             10258..11157
                     /gene="ybiY"
                     /locus_tag="ACHOWS_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFQ0047787.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11163..13595
                     /gene="ybiW"
                     /locus_tag="ACHOWS_002222"
     CDS             11163..13595
                     /gene="ybiW"
                     /locus_tag="ACHOWS_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFQ0047788.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13741..14556
                     /gene="ybiV"
                     /locus_tag="ACHOWS_002223"
     CDS             13741..14556
                     /gene="ybiV"
                     /locus_tag="ACHOWS_002223"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFQ0047789.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNS"
     gene            14708..15967
                     /locus_tag="ACHOWS_002224"
     CDS             14708..15967
                     /locus_tag="ACHOWS_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFQ0047790.1"
                     /translation="MTFSCETLPTDAKAAIREMKAALREQLSDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPREQAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWKSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(16208..17800)
                     /gene="ybiT"
                     /locus_tag="ACHOWS_002225"
     CDS             complement(16208..17800)
                     /gene="ybiT"
                     /locus_tag="ACHOWS_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFQ0047791.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIIGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18019..18939
                     /gene="ldtB"
                     /locus_tag="ACHOWS_002226"
     CDS             18019..18939
                     /gene="ldtB"
                     /locus_tag="ACHOWS_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFQ0047792.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18997..20115)
                     /gene="ybiR"
                     /locus_tag="ACHOWS_002227"
     CDS             complement(18997..20115)
                     /gene="ybiR"
                     /locus_tag="ACHOWS_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFQ0047793.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLMALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20112..20579)
                     /gene="mntR"
                     /locus_tag="ACHOWS_002228"
     CDS             complement(20112..20579)
                     /gene="mntR"
                     /locus_tag="ACHOWS_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFQ0047794.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVYMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20765..20893
                     /gene="mntS"
                     /locus_tag="ACHOWS_002229"
     CDS             20765..20893
                     /gene="mntS"
                     /locus_tag="ACHOWS_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFQ0047795.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21165..22748
                     /gene="opgE"
                     /locus_tag="ACHOWS_002230"
     CDS             21165..22748
                     /gene="opgE"
                     /locus_tag="ACHOWS_002230"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFQ0047796.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22797..23312)
                     /gene="ompX"
                     /locus_tag="ACHOWS_002231"
     CDS             complement(22797..23312)
                     /gene="ompX"
                     /locus_tag="ACHOWS_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFQ0047797.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23365..23430)
                     /locus_tag="ACHOWS_002232"
     CDS             complement(23365..23430)
                     /locus_tag="ACHOWS_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047798.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23665..24552
                     /gene="rhtA"
                     /locus_tag="ACHOWS_002233"
     CDS             23665..24552
                     /gene="rhtA"
                     /locus_tag="ACHOWS_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFQ0047799.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24852..25355
                     /gene="dps"
                     /locus_tag="ACHOWS_002234"
                     /gene_synonym="pexB"
     CDS             24852..25355
                     /gene="dps"
                     /locus_tag="ACHOWS_002234"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFQ0047800.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25759..26505
                     /gene="glnH"
                     /locus_tag="ACHOWS_002235"
     CDS             25759..26505
                     /gene="glnH"
                     /locus_tag="ACHOWS_002235"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFQ0047801.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26644..27303
                     /gene="glnP"
                     /locus_tag="ACHOWS_002236"
     CDS             26644..27303
                     /gene="glnP"
                     /locus_tag="ACHOWS_002236"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFQ0047802.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27300..28022
                     /gene="glnQ"
                     /locus_tag="ACHOWS_002237"
     CDS             27300..28022
                     /gene="glnQ"
                     /locus_tag="ACHOWS_002237"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFQ0047803.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            28139..30364
                     /gene="ybiO"
                     /locus_tag="ACHOWS_002238"
     CDS             28139..30364
                     /gene="ybiO"
                     /locus_tag="ACHOWS_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFQ0047804.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(30361..31287)
                     /gene="rlmF"
                     /locus_tag="ACHOWS_002239"
     CDS             complement(30361..31287)
                     /gene="rlmF"
                     /locus_tag="ACHOWS_002239"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFQ0047805.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
BASE COUNT         7679 a         8353 c         7771 g         7610 t
ORIGIN      
        1 tttggcaccg ctgatgtttt ttcaatatat tccgtatcga cagaatcaat aatattataa
       61 ttaccttcgc tattataaat aatcaatttt tcacgcgcga gttgattctt ggcaatgatc
      121 tcatgacctt gctttaaaaa tagaacttca tcattagcgc tgatgtgatc aagatactcg
      181 ttcagatatc gccagtttaa atcgacatga attttatcca ccactaacaa atcgtgatct
      241 ttgccgttga tgcgtagcgt aaaagagata cattttttac ccggacgatc aaagcaattg
      301 tatgccggat tccagctgtt gttagagtca tctgaagcca tcgaccacgg tcgtttcgat
      361 gggttaaaat cggcaaaaat aacccgccct gactcgtcga caagcggtcc atttacttgt
      421 tcctgatgat aaacaacgat tttatcattc gccctccgcg aaaagagata atatttatta
      481 tcatcaagta ccagtcccac cgagtctaaa aatggcatca tttccatttt cagaattacg
      541 gcgcgttgaa tctcctctgt attttttaat aaatatagtg agtcctttaa tgtcagggcc
      601 gaacgttcaa tatctttaaa gaaatagcca acgtttttct gaataagttc aaagcggcta
      661 tcggcacgtt ttaccgcgcg ttccgatacc agttgatagt taatgatgaa aaaaagacca
      721 ataaatgaca gtgctgccaa taaaaataaa attatcagcc ttatctttat cttttcgtat
      781 aaactcagca tgttaataca tttaacaaaa taactgtctg aacgctgtgc cgctgcaaaa
      841 tagtcccttg ctgcggcttt ttaaccaaat tacccgattc tacaaaatca gcagaaagtt
      901 acaacacttg cttagggcaa taatttcctg tgaggtgatt accctttcaa gcaatattca
      961 aacgtaacta tcctttaatt ttcggatcca gcgcatcgcg taaaccatcg cccaacaaat
     1021 tgaacgccag tacggtcaga aaaatagcca gggccggaaa aacagcgaca tgcggcgcga
     1081 taaccatatc cgctcgtgcc tcattgagca ttgcacccca ctctggtgtc ggcggctgcg
     1141 caccgaggcc gagaaatgac aggctggcgg cagagataat cgaggcgcca atgcgcatgg
     1201 tgaaaaacac cacgatagaa gagacggtcc caggcaagat atgacgcaac aaaatggtca
     1261 tatcgctggc accaatactg cgtgctgact cgataaaggt ttgctgtttc aacaccagcg
     1321 tgttgccgcg caccagccgg gcaaacgcgg ggatggaaaa aatggcgact gcgataatca
     1381 cgttagcaat gccgcttccc aacaccgcaa caacagcgat cgccagtaaa atacccggga
     1441 aggcaaacaa cacatcgcaa atgcgcatga tcagccgatc ccaccagcct tcataatatc
     1501 cagccagcaa gcccagcaac gtcccgatcg ccgcaccgat aaacacggca aacacgcccg
     1561 ccgccagcga tatttgcgca ccaaccagga cacggctgaa aatgtcgcgc cccagtgaat
     1621 cgacaccaaa ccagtgctgc aaagaaggtc cgttattcag attgtcataa tcaaaataat
     1681 tttcggcgtc atagggagcg atccagcgtg caaaaatggc caccacaatc aataaaataa
     1741 cgaacaatgc ggcggtcatc gccatatgct gacggcgaaa tcgtcgccag aattcatgcc
     1801 acggtgtacg tacctggtca ggtttgacca gtggcatggc gtttaacacc gcctgacgtc
     1861 gccagttaaa tagtcgcatc cttacttgta cctgatagcc gggttaatgg cggcgtaaag
     1921 cacatccacc actaagttga taagaataaa ttccagcgag aaaagcagaa tttccgcctg
     1981 aatcaccggg taatcgcgca tttcaacgga gtcaacgagt aagcgtccaa gtccgggcca
     2041 gttgaaaact ttctccacca cgatggaacc accgagtaaa aatccaaact gtaagcccat
     2101 catggtcact accgggatca tcgcgttgcg tagcccgtgt ttgaggacaa cccaggtttc
     2161 gctcacccct ttcgccctcg cggtacgcat ataatcttcg cttaaaacgt cgacaaacga
     2221 cgcgcgggta aagcgcgcca tcacggcggc cactgccgcg ccgagcgtca gggagggtaa
     2281 aatgtagtgc tgccaactgt ctgctcccac ggtaggcagc cagcccagtt caacagagaa
     2341 tacctgaatt aaaagcatcc ccagagcaaa tgccggaaac gaaatccccg acaccgcaat
     2401 agtcatactc aatcgatccg gccaacggtt acgccagacg gcggcgataa ttcccgccgc
     2461 cataccaaat atcaccgccc agaccatact ggttatggtc agccacagcg ttggcataaa
     2521 gcggctggca atctcatcgc caaccggacg acgcgacacc atcgacaggc caaaatcccc
     2581 ctgcacagca ttgctgatat agtgccagaa ctggtgatac agcggctgat ccaaccccag
     2641 ctgctgacgc accagttcta taacctgcgc atcagcttcg ggcccggcaa ttaatcgcgc
     2701 cggatcgccg ggcagcatgt ggacaaataa aaacaccagc accgagacga taaacagcgt
     2761 cggaatcaac cccagtaagc gtttgataac gtaattaagc attccactcc ctgcgttgct
     2821 tattgcaaat ccgcatcttc aaagctgaag ccggtgtctg gcatgatcca aaaaccggtc
     2881 aggtttttac tgtgtgccga cacaagtttt tctaccacca gcgggatcca cggcgactct
     2941 tgccagatga tatcctgcgc cgccttatac aagcgggtct tttccgccgg atcattagtt
     3001 ttcagtgcct gagccaggaa gtcatccacc tgtttattgc tgtaaaacgc ggtattaaac
     3061 agcgttggtg gccagttctg cgaggcaaac agcggcgata gagcccagtc ggcttcgccg
     3121 gttgaagccg accagccagt gtagaacatc cgcacgccgc tctctttttg ccctttacct
     3181 tcaacttctg ccgcccgctg tccggcatcc atcgcagtca cctgggcttt aatcccaacc
     3241 tgcgctaact gctgctgggt aaattgcagc actttctgcg cggtgctgtg gttatgtgac
     3301 gaccacagcg tggtactgaa accgttggga tatcccgcct ctttcagtaa ttcgcgcgct
     3361 ttcactggat cgtaaggcca cggtttataa ctttgcgcgt aggcgatact cggcggtacc
     3421 acaccagtag ctggcgttgc atagcccgcg aaggccactt tcaccaacgc cgggcggtta
     3481 atggcgtaat tcagcgcctc acggaccttc gggttatcga acggcttttg cgtcacgttc
     3541 atactgatat aacgctgcat aattgacgga ctggccatca actcgatatt tttgtttttc
     3601 tccagcagtg cggcctgctc gtagggaatg gggaaagcaa actgcgcttc accggtttgc
     3661 agcattgccg cgcgggtgtt gttatccgcc accggacgcc aggttatgct gtccagtttg
     3721 ggcaatcctg gctgccagta acccgcgaat tttttcacct tcacaaaatc ggtctgattc
     3781 caggtatcca gttcatacgg cccggttccc accggatgaa aaccaatctc cttgccatat
     3841 ttttccagcg ctgccggtga aatcatcgcg gtcgccggat gggcaagaat attaataaac
     3901 gctgagaatg gctgtttgag ggtaatcttt accgttgtcg gatcgatcgc ttccgttttg
     3961 gcaatattct tatacaggtt atagcgttta agatgattcg ccggatcgct ggcccggtcc
     4021 agattcgctt ttaccgccgc tgcgttgaag tcggttccat cctggaattt aatgccttcc
     4081 cgcagtttca cggtgtaagt aaggccgtca tcggaaacgg tataactctc cgccagcacg
     4141 tttttcagtt tcatctcttt atccagaccg aacagcccct ggtaaaacga tttcgctacg
     4201 gcctgagata acgtgtcatt cgcgtcatat ggatcgagcg tggtgaagtt cgatcctacc
     4261 gccaccacca catctttggc agcgaatgca caagatgcca tcaacgctgt cgcaatgccc
     4321 agcgccacta acccactacg gtgtacagct cttgccattt tattctccgc ctgaatgtta
     4381 tctacgcatg aatgcgtatt ctgattgtgg ttgtgcgacg taatgccccg gcccgacgca
     4441 ttgcaacgag acggctacaa cctcttcacc acgcagatga atattgctgg gaagatcgtc
     4501 cgacagcagt acacgctgcg gtcgttgtcg ggacggttca gcgaccggaa ctgccgccag
     4561 taatttacgc gtataaggat gctgcgggtt ttcgaagacc gcgcgccgtg gaccaatttc
     4621 aacaatttgt ccgcgataca tcaccgccac acgatgacta atccgctcta ccacggccat
     4681 atcgtgggag ataaacagat acgcaatgcc gaaatcacgc tggagatcga gcaacaagtt
     4741 gataatctgc ccgcgaatag aaacatccag cgccgagacg gcttcgtcgg caatgatcac
     4801 ttttggattc aatgccaacg cgcgagcaat gcagatgcgc tggcgctgac cgccggaaaa
     4861 ctcatgaggg taacgccagg catgttccgg taacaggccc acgcgctcca gcaaccacgc
     4921 aacgcgtgca gccgcttctt tacctggcaa taaaccgtgt acgcgcagcg gttcgataat
     4981 cgaatcaccg atggtctgac gtgggtccag cgaagcgtaa gggtcctgaa aaataaactg
     5041 aatatcccgg cgcaaagcct gaagtttacc ggatgacaag gtatcgattc gctgaccgtt
     5101 aaagataatt tcgccgccct gcgattcgac caggcgcagc aacgcccgcc cggtagtgga
     5161 tttaccgctg ccagactcgc ccaccagcga tagcgtttcg ccaggccaga gatcaaaact
     5221 gactttctca acggcatgca cttcccgcgt tacgcgattc aacaaaccgc tgcgcaaagg
     5281 gaaacgggtg accagattac gcacccgtaa aacaggttcg ccatccacca ccgttttctg
     5341 ctcgatgggg ggttcctgtt ttgctggatg ttcaagcgat atcaatggga aacgtcgggg
     5401 ataatctaac cctttcatcg caccaagttg cggaacagca gctaacagcg cacgggtata
     5461 aggatgttgc ggtgcatgaa aaatctgttc gacgctaccc gtttccaccg cctcgccctg
     5521 atacatcacc agtacccgat cggcaatctc tgccaccacg cccatatcgt gagtgataaa
     5581 gataacgccc atcgacatct ctttttgcaa tactttgatt aattgcagga tctgcgcctg
     5641 aatagtgaca tccaacgcgg tggttggctc atcggcaatc agcaccgccg ggcggcatga
     5701 cagcgccatc gcaatcatca ctcgctggcg catcccgcca gagagttgat gcggataacg
     5761 tgaaaggatg gtttgtgcct ccggaatgcg tacctgatcc agcatccgct tcgcctcgac
     5821 catcgcttct tcacgactgg cgttctgatg cagacgaatt gattcggcaa tctgttcacc
     5881 cacagtaaat accgggttca gcgatgtcat cggttcctga aatatcatcg ccatatccgc
     5941 accgcgcaca tggcgcattt gtgcagcgct ctgctcgcta agttcaatca cttcacgact
     6001 gcgccgccgc aacagcattt gatcgcactg cactaaaccg cccgcctgtt ccaacaagcg
     6061 catcaatgcc agcgcagtca ctgacttacc agagccggat tcgccaacaa ttgccagcgt
     6121 ctcaccgcgt tgcaggctaa aagagagatt gcggaccgca gctattttct gctggtcctg
     6181 cataaaggca atattcagat tttcaaccgc cagcacatca ccggcatcaa gttcatcact
     6241 gtgtggcaac ggtgtcccct ttttcacggt agataccggt ggttggcgta tcgcctgcgt
     6301 agccccaggc gcgatacatt ccttcggtgt taaacggtag cgcgacgtta ccttcatggt
     6361 cgatagcgat taagccaccg ctaccgccaa gcgcaggaag tttttccatt actacccgct
     6421 cgcaggcttc cgcgagactt aatccgccgt aatccattaa cgcggcgatg tcatatgccg
     6481 ccagcgcgcg gatgaagact tcgcccgtgc cggtacaaga aaccgccaca ctggcgttat
     6541 tggcgtagca tccggcaccc actaaggggc tatcgccaac tcgtccgggt aatttattgg
     6601 tcattccgcc cgtggacgta gctgccgcca gattgccgtc taaatccaac gccacggccc
     6661 ccacggtgcc cattttctgt ttttcatcca gtggcgcacc gctatggtcg agaaccattg
     6721 ccccttcctc gcgcgctgcc agtagttgtt cataacgcaa aggcgtggag aaaatctccg
     6781 gcgagacgcg ctccatgcca tgagcaaacg caaaattttc tgccccttcg ccaatcatca
     6841 tcacatgcgg gctttgctcc atcaccagcc gggcggcaag aaccggatta cgcagatgac
     6901 taacgcctgc caccgcaccg gctttcaggg tgttaccatc catcacacag gcgtccagtt
     6961 catgggtttc atcacgcgta aagacagcgc caattccggc gttaaacagt ggacactctt
     7021 ccagcagacg caccgcttcc gtcaccacat ccagcgcact ttcgcccgct tccagcattt
     7081 tctgcccggt ttcaacaatg gcagacagcg cctcgatgta gcgtaattcc tgttgcagac
     7141 tcatctgcgc gcggctaatt gcacctgcgc cgccatgaat tgcaatgact gctttgccca
     7201 tagtattcgt ccattaaggg tggctcgcca cttttgctat aaatatcaga atttttatga
     7261 attactcatt caatttttga atatagaaag atggatatgg catgtaaagg caggagccaa
     7321 cgctacgccc gaggtggcat acagtctgtg gcgtttttct gacataatag gcaaattcga
     7381 ttttgcctcc gcaggagtgt tttcatggaa tttaccaccg gattgatgtc gctcgacacc
     7441 gcgcttaatg agatgctttc tcgcgttacc ccactgaccg cccaggaaac gctgccactg
     7501 gttcagtgtt ttggtcgtat tctggcgagc gatgtcgttt cgccactgga tgtcccgggg
     7561 tttgataact ccgctatgga cggctacgcg gtgcgtttag ccgatattgc ctccgggcaa
     7621 ccgctgcccg ttgccggtaa atcctttgcc ggtcagccgt accatggtga atggcctgcg
     7681 ggtacctgca ttcgtattat gaccggtgcg ccggtgccag aaggctgcga agcggtggtg
     7741 atgcaggagc agactgaaca aacggacaat ggcgtgcgtt ttactgctga agtgcgtagc
     7801 gggcaaaata ttcgccgtcg cggtgaagat atctctgcag gtgcggttgt tttcccggcg
     7861 gggactcgcc tgactaccgc tgaactgcca gtgattgctt cgctagggat tgccgaagtt
     7921 ccggtgattc gtaaagtgcg tgtagcgctt ttttctaccg gtgatgaact ccagttgccc
     7981 ggtcagccgc tgggcgacgg ccaaatctac gataccaacc gtctcgccgt acacctgatg
     8041 ttagaacagt tgggatgcga ggtaatcaac ttagggatta tccgcgacga tccccatgcc
     8101 ctgcgcgccg catttattga agccgacagc caggcggatg tagtgatcag ttccggcggt
     8161 gtttcagtgg gtgaagcgga ttacaccaaa acgattcttg aagagctggg ggagatcgcc
     8221 ttctggaaac tggcgattaa accgggtaaa ccgttcgcgt tcggtaaact ttctaatagt
     8281 tggttctgcg gcctgccggg caacccggtt tcagcgacgc tgaccttcta tcaactggta
     8341 cagcctttgc tggcaaaact aagcggcaat accgccagtg gcctgcccgc gcgccagcgt
     8401 gtacgcacag cgtcccgcct gaaaaaaacg ccaggacgcc ttgatttcca gcgcggcgtg
     8461 ctgcaacgca acgccgatgg cgaactggaa gtgacgacca ccggacatca gggttcacat
     8521 atatttagct cctttagcct cggcaactgc tttatcgtgc tggaacgcga tcgcggcaat
     8581 gtggaagtgg gcgaatgggt ggaagtagaa ccgtttaacg cgttgttcgg aggcctgtaa
     8641 tggcggaact cagcgatcag gagatgctgc gctacaaccg gcaaatcatt ctgcgcggtt
     8701 ttgattttga cggtcaggaa gcattgaaag attcccgcgt gctggtcgtc ggccttggtg
     8761 ggctcggctg cgcagcttcg cagtatctgg caagcgccgg tgtcggcaac ctgacgctgc
     8821 tcgacttcga cacggtttcg ctctccaatc tgcaacgcca gacgctgcac agtgatgcca
     8881 cggtcgggca accaaaggtg gaatccgccc gtgacgccct gacgcggatc aacccacata
     8941 tcgcgattac gccagtcaat gcgctgcttg atgacgctga acttgctgcg atgattgcaa
     9001 aacacgatct ggtgctcgac tgtacggata acgttgcagt acgtaatcaa ctgaacgcag
     9061 gctgttttgc cgcgaaggtg ccgctggttt ccggcgcggc aattcgtatg gaagggcaaa
     9121 tcacggtctt cacttatcag gacggcgaac cgtgctatca ctgccttagc cgtttgtttg
     9181 gcgagaacgc gttaacctgc gtagaagcag gcgtgatggc accgttaatc ggcgtaattg
     9241 gttcgttgca ggcaatggaa gcaatcaaac tgctggcagg ttatggcaaa ccagccagcg
     9301 gaaaaatcgt gatgtacgat gcgatgacct gtcagtttcg cgaaatgaaa ctgatgcgca
     9361 atccggggtg tggggtgtgc gggcagtaac tgccacctcc aggccagata agacgcggca
     9421 ctccgccacg tcatcagcaa tttatgagga tgcagaacgt ccggttaaat cgacgttctg
     9481 ccaaacgctc ccagccagtc ctgttcaaac ttcgccacag cggcatcaac cgccggatag
     9541 ctaatcatct gttgtgccac atccagcggc agagtaattg attcacatcc tgccagtaag
     9601 cagtccagcg cctgacgcgg ggttttgaaa ctcgcggcta acactttcgc ctgcggcgca
     9661 tgcattttca ataactggtg taagtcggtc acagtctgaa taccgctacc gccctgagca
     9721 tcaatacgat taacgtaagg cgcaacatat tccgcacctg ccagcgccga cagtagccct
     9781 tgtgctgcgc catataccgc ggttcccagc gtcggaatcc cttccgcttt taacatctta
     9841 atagctgcca gcccctcagc ggtcaccgga accttcacca cgatatccgc aataatagaa
     9901 cgcaatttac gtgcgtcatt aaccatccct tcagcagtgg tagccattac ctgggcaaac
     9961 agacgcccct gaccgcccat cgcctcgtga agttgcggaa gcacaacctc cagcggtttt
    10021 ttacccgctg cgataatgct tgggttagtg gtcacacccg ccagcggaaa aatacgtgac
    10081 agcgtcttca ccgcaacaac gtctgaagta tccagataca gttccataac catcctcaaa
    10141 atgtactttt tgtctgaatg tagactatca cgggaaaact ctctgaacag ttgacgcaca
    10201 tcaatctaac tttcattcga aagtatttta atgtttgaac gaaacagaga ggcaactatg
    10261 attttcaata ttcaacgcta ctcgacccat gatggccccg gtatccgcac ggtcgtattt
    10321 cttaaaggct gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg
    10381 caggatctgc tgtatgaccc acgactctgt ctggaaggct gtgagctgtg cgctaagacc
    10441 gcaccggaag tgattgaacg cgcgttgaat ggtttgctta ttcatcggga aaagttaacc
    10501 ccggagcatt ttacggcgct aaccgactgc tgcccgacac aggcgttaac cgtgtgtggt
    10561 gaagtgaaaa gcgttgagga gatcatggca accgttttgc gcgataaacc gttttacgat
    10621 cgcagcggcg gcggcttaac gctttctggc ggtgaaccgt ttatgcagcc ggaaatggcg
    10681 acggcgctgc tgcaagccag ccacgaggcc ggcattcata ctgcggtaga aacctgtctg
    10741 catgtgccgt ggaaatatat cgccccttct ctgccctata tcgatctgtt tcttgccgat
    10801 ttaaaacacg ttgccgacgc gccgtttaaa cagtggaccg acggtaacgc cgccagggtg
    10861 ctggataacc tgaaaaaact cgccgcagcg ggcaaaaaaa tcattatccg cgtgccactg
    10921 attcagggct ttaatgccga cgaaacctct gtgaaagcca ttaccgattt tgccgccgac
    10981 gagctgcacg ttagcgaaat tcatttcctg ccctaccaca cgctgggcat caacaaatat
    11041 cacttactta atctgcccta tgacgccccg gaaaaaccgc ttgatgcgcc agaactgctc
    11101 gactttgccc agcagtatgc ctgccagaaa gggttaaccg cgaccttacg aggataacaa
    11161 tcatgacaac actaaaactg gatacgctca gcgaccgcat taaagcgcac aaaaatgcgc
    11221 tggtgcatat tgtgaaaccg ccggtctgta ccgaacgagc acaacactat accgagatgt
    11281 atcaacaaca tctcgataag ccaatcccgg tacgtcgcgc gctggcactg gcgcatcacc
    11341 tggcgaatcg caccatctgg atcaaacatg atgaattgat cattggcaac caggcaagcg
    11401 aagttcgcgc cgcgccgatc ttcccggaat ataccgtctc ctggatcgaa aaagagattg
    11461 atgatctggc agatcgtccc ggtgctggtt ttgcggtgag cgaagagaac aaacgcgttc
    11521 tgcatgaagt gtgcccgtgg tggcgcggtc agaccgtaca ggatcgctgc tacggcatgt
    11581 ttaccgatga gcaaaaaggt ctgctggcga ccggcatcat taaagcggag ggcaatatga
    11641 cctccggcga tgcgcacctg gcggtcaatt tcccgctact gctggaaaaa ggtcttgatg
    11701 gtctgcgtga gaaagtggcg gaacgtcgct cgcgcatcaa tctgacggtt ctggaagatc
    11761 tgcacggcga gcaattcctg aaagcgattg atatcgtgct ggtggcagtc agtgaacaca
    11821 ttgaacgttt cgctgccctg gcgcgtgaaa tggccgcaac cgaaacccgc gaaagccgtc
    11881 gcgatgaact gctgacgata gcagaaaact gcgatcttat cgcccaccag ccgccgcaga
    11941 ctttctggca ggcgctgcaa ctgtgctact tcatccagtt gattttgcag atcgaatcta
    12001 acggtcactc ggtatcgttt ggtcgtatgg accagtatct ctacccatac tatcgccgtg
    12061 acgttgaact gaaccagaca ctggatcgcg aacacgccat cgagatgctg catagctgct
    12121 ggctgaaact gctggaagtg aacaaaatcc gctccggctc acactcaaaa gcctctgcgg
    12181 gaagtccgct gtatcagaac gtcaccattg gcgggcaaaa tctggttgat ggtcaaccga
    12241 tggacgcggt aaatccactc tcttatgcga tcctcgaatc ttgcggtcgc ctgcgttcga
    12301 ctcagcctaa cctcagcgtg cgttaccatg cgggtatgag taacgatttc ctcgacgcct
    12361 gtgttcaggt gatccgctgc ggcttcggga tgccggcgtt caacaacgac gaaatcgtga
    12421 tcccggaatt tattaaactc ggtattgaac cgcaggacgc ttacgactac gcagcaattg
    12481 gttgtatcga aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc atgagcttta
    12541 tcaacttcgc ccgcgtgatg ctggcggcgc tggaaggtgg tcgtgatgcc accagcggca
    12601 aagtgttcct gccacaagca aaagcgttgt cagcgggtaa cttcaacaac ttcgatgaag
    12661 taatggacgc atgggatacg caaatccgtt actacacgcg taaatcaatc gaaatcgaat
    12721 atgtcgtcga taccatgctg gaagagaacg tacacgatat tctctgctcg gcgctggtcg
    12781 atgactgtat tgagcgagcg aaaagtatca agcaaggcgg cgcgaaatat gactgggttt
    12841 ctggcctgca ggtcggcatt gctaacctcg gcaacagcct ggcggcagtg aagaaactgg
    12901 tatttgaaca gggcgcgatt ggtcagcaac agctggctgc cgcactggcg gatgacttcg
    12961 acggcctgac tcacgagcag ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca
    13021 acgacgatga tactgtcgat acgctgctgg ctcgcgctta tcagacctat atcgacgaac
    13081 tgaagcagta ccataacccg cgctacggtc gtggtccgat tggcggcaac tattatgcgg
    13141 gcacatcgtc tatctccgct aacgtaccgt ttggcgcgca gacgatggca acgccggacg
    13201 gacgtaaagc acataccccg ctggcagaag gcgctagtcc ggcctccggt actgaccatc
    13261 tcggccctac tgcggtcatt ggctcggtgg gtaaactgcc tacggcagca attctcggcg
    13321 gcgtgctgct caaccagaaa ctgaatccgg caacgctgga gaatgaatct gacaagcaga
    13381 aactgatgat cctgctgcgt accttctttg aagtgcataa aggctggcat attcagtaca
    13441 acattgtttc ccgcgaaaca ttgctggagg cgaagaaaca tccggatcaa taccgcgatc
    13501 tggtggtacg cgtagcgggt tattccgcct tcttcaccgc gctctctcca gacgcacagg
    13561 acgatatcat cgcccgtacc gaacacatgc tgtaatcccc tcagcccggc gtcgtcgtcg
    13621 ccgggccaaa tcattcacat catcaatttc agcctcgctt tcattcgaaa tataatttgt
    13681 gctctgcgtc acattgttat tacactgtta cggtttcatt ttcgagccag gagcacacct
    13741 atgagcgtaa aagttatcgt cacagacatg gacggtactt ttcttaacga cgccaaaacg
    13801 tacaaccaac cacgttttat ggcgcaatat caggaactga aaaagcgcgg cattgagttc
    13861 gttgtcgcca gcggtaatca gtattaccag cttatttcgt tctttcctga gctaaaggat
    13921 gagatctctt ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa gcagttgttc
    13981 cacggcgaac tgacccgaca tgaatcgcgg attgttattg gcgagttgct aaaagataag
    14041 caactcaatt ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa
    14101 gcattcgtcg cactgatggc aaaacactac catcgcctga aacctgtaaa agattatcag
    14161 gagattgacg acgtactgtt caagttttcg ctcaacctgc cggatgaaca aatcccgtta
    14221 gtgatcgaca aactgcacat agcgctggat ggcattatga aacccgtcac cagtggtttt
    14281 ggctttatcg acttgattat tcccggtcta cataaagcaa acggtatttc gcggttactg
    14341 aaacgctggg atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg
    14401 gagatgctga aaatggcgcg ttattccttt gcgatgggca atgctgcgga aaacattaaa
    14461 caaatcgccc gttacgctac cgatgataat aatcatgaag gcgcgctgaa tgtgattcag
    14521 gcggtgctgg ataatatcgc tcctttcaat agttgatctt ccccctggcc gaagtgacca
    14581 cttcggcctc tctccttttt gctcgctttg tgcactgact caatattttt atcttgcgtt
    14641 aacttatttt taacttaatg tatcaatcgc aatcatttta ctttcgaatg aaagaagcga
    14701 ggttgatatg actttttcct gtgagacgct gcccacagat gcgaaagcag ctatccgaga
    14761 gatgaaagct gcgctgcggg aacagctcag tgatgtgcaa caggttttcg atacactctc
    14821 tgcaaaaatc gaaacgcgtg ttgctgaaat cgatgcccta aaagcgcaag ggctgccggt
    14881 ttggccggaa gtttcttata gtgatattgc cgcaggtaca gtaagcgatg ccactcgcaa
    14941 tgaaattaaa cgtcgcggtt gtgcagtcat aaaaggccat ttccctcgcg agcaggcgat
    15001 ggcgtgggat cgtgccatgc tcgattatct tgatatcaat cattttgatg atgtttataa
    15061 aggccctggc gacagttttt tcggctcgct ggaggcttct cgtcccgaaa tctatcccat
    15121 ttactggtcg caagcacaga tgcaggcgcg gcagagcgaa aacatggctg ctgtgcaatc
    15181 ttttctaaac cgactgtgga agtctgaaag caacggcaaa cagtggttta atcctgatat
    15241 cagtgtgatt tatccagaca ggatccgccg ccgccctcca ggtactacat ctaaaggcct
    15301 gggcgcgcat accgattctg gtgcgctgga acggtggtta ttacccgctt atcagcgcgt
    15361 ttttgccaat gtgtttaatg gtaatttcga tgattacgat ccctgggatg ctgcacaccg
    15421 gaccgatgtc gaagagtaca cggtagataa cactacaaaa tgttcggtgt tccgcacgtt
    15481 tcagggctgg acggcgctct ctgatatgtt atcgggtcag ggattgttac atgtggtgcc
    15541 tatcccggaa gcgatggcgt atgttttatt gcgcccgctg ctggatgacg taccggacga
    15601 tgaactgtgc ggggttgcac ctggcaaagt tctgcccatt tcagagaaat ggcatccact
    15661 actgcttaaa gcgctgactt ccattcccgc gataacagca ggggattccg tctggtggca
    15721 ctgcgatgtg attcactccg tcgcgccagt agacaatcaa cagggttggg gcaacgtgat
    15781 gtatatccct gctgcgccaa tgtgcgagaa aaatcgggct tatgcacaca aagtcaaaat
    15841 ggcgctggaa aaaggcgcat cacctggcga cttcccgcgg gaagattatg aagcaaactg
    15901 ggaaggccgc tttacgctgg aggatctgaa cattcacggt aagcgcgcac tgggcatgga
    15961 tgtttgagag ttaatggaag tgaattgatg tgggattaag aaaatcgatt caacgttact
    16021 cagattgctg acaaatgcgc tttgttaatg cctgatgcgg cgttaacgcc ttatccggcc
    16081 tacaaagtcc tgcagattca atatattgca ggtactgcgt aggcctgata agcatagcgc
    16141 atcaggcaat cttgcgtttg tcatcagccg ccgtcacccc gacagtatcg ggctgacgga
    16201 taataactta ctcgatccct ttactacgca gataatcttc gtaattaccg ctaaagtcga
    16261 tcacgcgttc cggggtgatt tccagaattc gggtcgccag ggagcttaca aactcacggt
    16321 cgtgagacac aaagatcagc gtgccctgat acagttccag cgccatgttc agcgactcaa
    16381 tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc ggcttctgca
    16441 tcattaactt accaaacagc atccgccctt tctccccacc ggaaagcact ttcgctggct
    16501 ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc gcctgctcgt
    16561 catcgccttc ctgcttccac tgactcatcc attcgaacac ggtaagatca ttttcgaact
    16621 catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat tttacggtgc
    16681 cgctgtccgg ttgcagatcg cccaccagcg ttttcaacag tgttgattta ccgacgccgt
    16741 tggtacccag taccgccagt ttttcaccga cttccagcag caggttgaga tttttaaaca
    16801 gcggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta cggaacagtt
    16861 tcttatcctg ttcaaaacgg atgaacgggt tctgacggct ggaggctttc acctcttcca
    16921 gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg
    16981 cactaaagcg gctaacgaaa gattgcagct cagcaatctg cgctttcttc ttggcgttat
    17041 cagccagcag acgctcacgc gcctgggtcg ccgccgtcat gtactcatcg tagttacctg
    17101 gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacaaacc atgttaagga
    17161 agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc acctgttcca
    17221 gccaacgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg agaatgtccg
    17281 gatcagcaaa cagcgcctgc gccagcagca cacgcagctt ccagccagga gcaacttcac
    17341 tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacagt tcaccggcgc
    17401 gagcttccgc agagtaaccg tccatttcgc cgtatttcac ttccagatcg gccactttat
    17461 agccgtcttc ttcactcatt tccggcaaag catagatgcg gtcacgctcc tgcttcactt
    17521 cccacaactc tttatgccca atgatcaccg tatccagcac agtgaactct tcaaaggcaa
    17581 actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg ttacccaacg
    17641 tcggctctaa gtcgccgccg aggatcttca taaaggtgga tttaccacta ccgttcgcgc
    17701 caatcaggcc gtaacggttg ccgccgccaa atttgacgga aatgttttca aacaacggct
    17761 tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc tgaaaagaga
    17821 tatgacaaac cgcgcattat gccataaatc tgaaagaaga tcctgctcct cgcttaaggt
    17881 ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc actgtgagct
    17941 ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg gcctgccagg
    18001 cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct
    18061 gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac cgacgggagt
    18121 cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca gccgctggag
    18181 tattttgccg ccgagtacca gatgggactt tccaatatga tggaagcgaa cccgggtgtg
    18241 gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct gatcctgccg
    18301 gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta ttactatccg
    18361 aaaggaacca acaccgttat cgtgctgccg atcggcattg gtcagttagg caaagacacg
    18421 cctatcaact ggaccaccaa agttgagcgt aaaaaagcag gcccgacctg gacgccgacc
    18481 gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt cgttccggca
    18541 ggtccggata acccgatggg tctgtatgca ctctacatcg gtcgcctgta cgctatccat
    18601 ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggctgtgt gcgtctgcgt
    18661 aacgaagaca ttaaattcct gttcgagaaa gtgcctgtcg gtactcgcgt gcagtttatt
    18721 gatgagccgg tgaaagcgac taccgaacca gacggcagcc gttacattga agtccataac
    18781 ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa ttgtgccaat taccctgacg
    18841 aagagcgtgc agacagtgac cggtcagcca gatgttgacc aggttgttct tgatgaagcg
    18901 atcaaaaatc gttccgggat gccggttcgt ctgaattaat cgtcaaaacg taaaacaaag
    18961 gcggactcat aatccgcctt ttttatttgc cagaccctac caggccggga tcataaccaa
    19021 caaaacatat cccaccagcg ccgcccacaa cagcatcggt atcgaataaa gatgaaaacg
    19081 ccaccagata cggcgatcgt tcgccatccg taacgcaatc aaatttgcca gcgatccggg
    19141 taataaacca aagcccccta cgtttaccgc ccatgccagc agcaacgacg gcggcacata
    19201 gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac cgattgccgt
    19261 taaccataat ccgggttcgg ataaatgact cacgttactt aacaccccct gcaaagctgg
    19321 cagttgggtt agtaaatgga cgtcgataaa catcgccata aacaccagca gcagcgtcca
    19381 gtccacactg agcaccacgc gacgtgccag cagcgcaaag cccaccgcca caatcaccag
    19441 tccccacagc tcttgtttta actccagcgc catcagaaag acgatataca gccccagaca
    19501 actccacacc agccgcggtt tccactccgg cgtttgcgcc cccgtatggt attgcagcgc
    19561 ctttccaggg aaacaacacc agcacaacag caggagtgtc agcatcattg cgccagccag
    19621 cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc agataagaat
    19681 attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca gcgcctcaaa
    19741 aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag tcagcggaac
    19801 aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg ccgccagcac
    19861 cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc gccccagcac
    19921 atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc ttaaggtgat
    19981 gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggaa cgaaaaaact
    20041 taatccgatg ccaacaagaa ttaataactg aaaaaaacga tcgccttgca gcgtgcgtaa
    20101 aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaggc atccagcgtc
    20161 tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc cggactgacg
    20221 cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc ctgcgccagt
    20281 ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat cagccccatg
    20341 gttgccagtc gcttaagcat tttagccacc gtcggttgcg aaactcccaa acgagcagcc
    20401 atgtatacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa
    20461 tcatcaatca gctcgcgccg atgcgcctct ctgacctggc ggaacccttc aacgtgctct
    20521 tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg acgactcatt
    20581 gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca cattgtaaac
    20641 cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctatataat atagcctgtg
    20701 ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca agtcaggagg
    20761 tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg
    20821 gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc
    20881 ttcagataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg caggcataac
    20941 gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc gcaaaagatt
    21001 tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc cctgcgtata
    21061 ttgcggctcg cggatgcgga cccctttccc ctctccacgc actcttgcag gtattgaccc
    21121 ttgacgccaa ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat ttaaccctca
    21181 aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc tactttttac
    21241 agtcgctatt aattaacctc ggcttaggtt atcccttcag tttgctctac accgctgcgt
    21301 ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg
    21361 gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac ggcgcaccta
    21421 atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc gaaatcctga
    21481 cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc ggcgtaatag
    21541 caatcaggcg gaaaaaagag agtgaaaaag cgcgctggaa taccttcgac agcctgtgtc
    21601 tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc tggggtggcg
    21661 tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat gtcatcgtca
    21721 ataataacga ggtgattgaa gagcaagaac ggatggcaaa actgtccgga atgaaagata
    21781 cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg atcggagaaa
    21841 gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca
    21901 gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc acgcagaaat
    21961 cgcttggctt aacgctcaat cgcgttgtcg atggcaaacc acagtttcag gataactttg
    22021 tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag ggccaaatcg
    22081 gcgaatacga taccgccatc gccagcatcg ccaaacgtgc agatgaagtg tacttcctga
    22141 aagaaggtaa ttttgaagca gataaaaaca cgaaagacga agcgttactg gatatgaccg
    22201 ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttttacat ctgatgggct
    22261 cacatccgca ggcctgcgac cggacacaag gcaaatacga aacctttgtg caatcgaaag
    22321 aaacgtcgtg ctatctctat accatgacgc aaacagacga tttactgcgt aagctgtacg
    22381 atcagttacg caacagcggc agtagcttct cgctggttta cttttctgac cacggtctgg
    22441 cctttaaaga gcgtggcaaa gacgtgcaat accttgccca tgatgataag tatcagcaaa
    22501 atttccaggt gccttttatg gtcatttcga gcgacgataa agcgcatcgg gtgattaaag
    22561 cccgccgctc tgccaatgac ttcttaggct ttttctccca gtggacggga attaaagcga
    22621 aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg atatacatca
    22681 ccaacttcca gttacagaag gtagattaca accatctcgg aaccgatatt ttcgacccga
    22741 aaccttaaaa acaaaaatcc gccccgagag gcggattttt tatatcaccg aagtgattag
    22801 aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat acggctctgc
    22861 tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc agcaccgtag
    22921 gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt ctggaattta
    22981 ccataaccca cacccactac accgtagatg cttgcccagt cgttaatgcg gtaagccgga
    23041 ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc agtacggctt
    23101 ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc ttcatagcgg
    23161 tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct ctgtgcgtaa
    23221 ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc cagaactgcg
    23281 gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat tcaaataagt
    23341 cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt gatgtctaac
    23401 gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat ttaaatcact
    23461 ttttcagaga gctgcataag tattacgcat gttttccctg tcattcatcc agattattcc
    23521 taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat
    23581 tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg ctttacttag
    23641 gtttagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc ggtctggtta
    23701 ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcctc gttagctaag
    23761 tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg
    23821 ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga gcagcggtta
    23881 ccgctgttgt tttacggcgt ttcgctgggg gggatgaatt atctttttta tctttctatt
    23941 cagacggtac cgctgggtat tgcggtggcg ctggagttca ccggaccact ggcggtggcg
    24001 ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg ttctggcggt tcttggtctg
    24061 tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg
    24121 gcactgggag caggggcttg ttgggctatt tacattttaa gtgggcaacg cgcaggagcg
    24181 gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat tttcgtgcca
    24241 attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc attgggtctg
    24301 gcggtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc cctcacccgt
    24361 ttgccaacgc ggacatttgg tacgctgatg agcatggaac cggcactggc tgccgtttcc
    24421 gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct cggcgcaatt
    24481 atcgccgctt caatgggttc tacgctgaca gtacgcaaag agagcaaaat aaaagaatta
    24541 gacattaatt aaatttacat ttctgcatgg ttatgcataa ccatgcagaa tttatcgcta
    24601 cttttcctct acaccgtctt tatatatcga attatgcaaa agcatattta ttccgaaaat
    24661 tcctggcgag caaataaata agaattgttc ttattaatat atctaactca ttgagttttt
    24721 attggttttg ttttttcacg cttgttacca ctattagtgt gataggaaca gccagaatag
    24781 cggaacacat agctggtgct atacttagtc tcgttaatta ctgggacata acatcaagag
    24841 gatatgaaat tatgagtacc gctaaattag ttaaatcaaa agcgaccaat ctgctttata
    24901 cccgcaacga tgtctctgat agcgagaaaa aagcaacagt agagttgctg aatcgccagg
    24961 ttatccagtt tattgatctt tctttgatta ccaaacaagc gcactggaac atgcgcggcg
    25021 ctaacttcat tgccgtacat gaaatgctgg atggcttccg cactgcactg atcgatcatc
    25081 tggataccat ggcagaacgt gcagtgcagc tgggcggtgt agctctgggg accactcaag
    25141 ttattaacag caaaactccg ctgaaaagtt acccgctgga catccacaac gttcaggatc
    25201 acctgaaaga gctagctgac cgttacgcaa tcgtcgctaa tgacgtacgc aaagcgattg
    25261 gcgaagcgaa agatgacgac accgcagata tcctgaccgc cgcgtctcgc gacctggata
    25321 aattcctgtg gtttatcgaa tctaacatcg aataaatcca tcgctgatgg tgcagaactt
    25381 tagtacacga taaaggcggc ttcctgacag gaggccgttt tgttttgtag cccgcctcaa
    25441 cgcacttatt tagtgcatcc atctgctatc ttcagctgat taagtaaatt ttttgtatcc
    25501 acatcatcac acaatcgtta cataaagatt gttttttcat caggttttac gctaaataat
    25561 cactgtgttg agtgcacaat tttagcgcac cagtttggtg ccccagaatg gtgcatcttc
    25621 agggtattgc cctataaatc gtgcatcacg tttttgccgc atctcgaaaa atcaatgagt
    25681 tgcaaaactg gcacgatttt ttcatatatg tgaatgtcac gcaggggatc gtcccgtgga
    25741 tagaaaaaag gaaatgctat gaagtctgta ttaaaagttt cactggctgc actgaccctg
    25801 gcttttgcgg tttcttctca tgccgcggat aaaaaattag ttgtcgcgac ggataccgcc
    25861 ttcgttccgt ttgaatttaa acagggcgat aaatacgtgg gctttgacgt tgatctgtgg
    25921 gctgccatcg ctaaagaact gaagctggat tacgaactga agccgatgga tttcagtggg
    25981 atcattccgg cactgcaaac caaaaacgtc gatctggcgc tggcgggcat taccatcacc
    26041 gacgagcgta aaaaagcgat cgatttctct gacggctact acaaaagcgg cctgttagtg
    26101 atggtgaaag ccaacaataa cgatgtgaaa agcgtgaaag atctcgacgg gaaagtggtt
    26161 gctgtgaaga gcggcactgg ctccgttgat tacgcgaaag caaacatcaa aactaaagat
    26221 ctgcgtcagt tcccgaacat cgataacgcc tatatggaac tgggcaccaa ccgcgcagac
    26281 gccgttctgc acgatacgcc aaacattctg tacttcatca aaaccgccgg taacggtcag
    26341 ttcaaagcgg taggtgactc tctggaagca cagcaatacg gtattgcgtt cccgaaaggt
    26401 agcgacgagc tgcgtgataa agtcaacggc gcgttgaaaa ccctgcgcga gaacggaact
    26461 tacaacgaaa tctacaaaaa atggttcggt actgaaccga aataataacg ctacgcctgt
    26521 aaggcgcact ggcagttccc tctcccctct ggggagagga ttagggtgag gggcgcaaac
    26581 ccgctccggg gccattaatt accctgaatt tgattattta caccacggta acaggaacaa
    26641 catatgcagt ttgactggag tgccatctgg cctgccattc cgcttctgat tgaaggtgcc
    26701 aaaatgaccc tgtggatctc ggtcctcggt ctggctggcg gcctggtgat cggattgctg
    26761 gcaggttttg cacgcacctt cggtggttgg atagccaacc acgtcgcgct ggtctttatt
    26821 gaagtgatcc gcggcacacc tatcgtcgtc caggtgatgt ttatctattt cgccctgccg
    26881 atggcattta acgacttacg catcgaccca tttactgcgg cggtggtcac catcatgatc
    26941 aactccggcg catacattgc ggaaatcacg cgtggtgcgg tgctgtctat tcacaaaggt
    27001 tttcgtgaag caggactggc gctcggtctt tcacgttggg aaaccattcg ctacgtcatt
    27061 ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta accagtggat catcagcatt
    27121 aaagacacct cgctgtttat tgttatcggc gtggcggaac tgacccgtca ggggcaagaa
    27181 attattgccg gtaacttccg cgcccttgag atctggagcg ccgtggcggt gttctatctg
    27241 attattaccc tggtgctgag ctttattctg cgtcgtctgg aaagaaggat gaaaatcctg
    27301 tgattgaatt taaaaacgtc tccaagcatt ttggtccaac ccaggtgctg cacaatatcg
    27361 atttgaacat tgctcagggc gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat
    27421 cgaccctgct gcgctgcatc aacaaactgg aagaaatcac ttccggcgat ctgattgtcg
    27481 atggcctgaa ggttaacgat ccgaaagttg acgagcgcct gattcgccag gaagcaggta
    27541 tggtgttcca gcagttttac ctcttcccgc atctgacggc gctggaaaac gtcatgtttg
    27601 gtccgctacg cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg cgtgagctgc
    27661 tggcgaaagt cggtctggca gaacgtgcac atcactaccc ttccgaactt tctggtggtc
    27721 aacagcagcg tgtggcgatc gcacgcgcgc tggcggtgaa gccgaaaatg atgctgtttg
    27781 atgaaccgac ttccgctctt gacccggaac tgcgccatga agtgctgaag gttatgcagg
    27841 atctggctga agaagggatg acgatggtga tcgtgacaca cgaaatcggt tttgccgaga
    27901 aagtagcttc gcgcctgatc tttatcgaca aaggccggat tgcggaagat ggcgatccgc
    27961 aggtgttgat caagaaccca ccgagccagc gcttgcagga atttttgcag cacgtctctt
    28021 aacttctcag cctccctttc ctccgaaagg gaggcacacc agattcctct catttaaaat
    28081 cgcccctcct ccagcatcta tacttatctt tttgctctgt tttctcactg gaggagtcat
    28141 gcggtggatc ctgttcatcc tcttctgcct gctgggcgca cctgcccacg cggtatccat
    28201 acccggcgtt acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga
    28261 tatcgaacaa aaaaaagcgg cctatggtgc actggcggat gtgctggata atgacacctc
    28321 acgtaaagag ttgatcgacc agttgcgcac cgtcgccgcg acgccccctg cggaacccgt
    28381 accgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa aagtcaccga
    28441 agtcagccgc cattatggtg aagccctttc cgcccgcttc ggacaacttt atcgcaatat
    28501 caccggctcc ccgcataagc cgtttaatcc acaaaccttc agcaatgcgt tgacccattt
    28561 ttcaatgtta gcggtattag tgtttggttt ttactggctg attcgcctgt gcgcacttcc
    28621 gctgtatcgc aaaatgggcc agtgggcgcg gcaaaaaaat cgtgagcgca gtaactggtt
    28681 gcagcttccg gcgatgatta tcggggcgtt tattatcgac ctgctgttac tggcactgac
    28741 attatttgtc ggtcaggttt taagcgacaa cctgaatgcg ggcagtcgca ccatcgcttt
    28801 ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag ctgtactacg
    28861 cctgattttt tgcccaaatg tggcggagct gcgcccgttc acgattcatg acgagaccgc
    28921 ccgttactgg agccgtcgcc tgagctggtt aagcagtttg ataggttatg gcctgattgt
    28981 ggccgtgccg attatctcta atcaggtgaa tgtacaaatt ggcgcgctgg cgaacgtcat
    29041 cattatgctg tgcatgacca tctgggcgtt gtacctgatc tttcgtaata aaaaagagat
    29101 tacccaacat ttgctcaact ttgcggagca ttcgctggcc tttttcagct tgtttatccg
    29161 cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcgtgc tgtttttctt
    29221 ttcgttgttc gatccgggca acagcctgaa atttatgatg ggtgcaacgg tgcgcagcct
    29281 ggcgattatt ggtatcgcag catttgtttc cggtatgttt tcccgctggc tggcgaaaac
    29341 catcaccctc tcgccacata ctcagcgaaa ttatccggag ctgcaaaaac ggctgaatgg
    29401 ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg cggtaatgct
    29461 gctgttgagc gcatggggat tattcgattt ctggaactgg ctgcaaaacg gtgcggggca
    29521 gaaaaccgta gatatcctga tccgtatcgc actcattctt ttcttctcgg cggttggctg
    29581 gacggtgctc gccagtttga tcgaaaaccg gctggcttcg gatattcatg gccgcccact
    29641 acccagcgcc cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg cggtgattat
    29701 cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gttaatatcg cgccattgct
    29761 ggcaggtgcc ggggcattag gtctggctat ctcttttggt tcgcaaacgc tggtgaaaga
    29821 tattatcacc ggggtattta ttcagtttga aaacggcatg aacactggag atttggtgac
    29881 tatcgggccg ttgaccggca ctgtggaacg gatgtcgatt cgctccgtgg gcgtgcgcca
    29941 ggataccggg gcgtatcaca tcattccgtg gtcttcgata accacctttg ctaacttcgt
    30001 ccgcggtatt ggttcggtag tggcaaacta cgatgttgat cgccatgaag atgctgataa
    30061 agccaatcag gcactgaaag atgcggtagc agaattgatg gaaaacgaag aaattcgcgg
    30121 gctggttatt ggtgaaccga actttgccgg gattgtcggc ttaagcaata ccgcgtttac
    30181 actgcgtgtt tcgttcacca cgctgccact caaacagtgg acggtccgct ttgccctcga
    30241 cagccaggtg aaaaaacatt tcgacctggc gggtgttcgc gcgccggtgc agacttatca
    30301 ggtgctgcca gctccgggcg cggccccggc tgaaccgttg ccgccggggg agccaacgct
    30361 ttaacgctgg cgattgacaa aacggcggcg ctgctcgtcg ttcataaagg tccaggctat
    30421 aaagcggctc tgcttttgcc cctgggccat ctcttttttt accaccttca ccgcacctac
    30481 gtccgtcaaa gcacgataca acggcggtaa gttttcacca cgagatacca gcgaggtaaa
    30541 ccacatcacc tgcttcgcga agcctttgct ctcttcaatc atctttttga taaaagcgac
    30601 ctcaccgcct tcacaccaca actcctgttg ctgaccacca aagttcagtg catcgtcttt
    30661 gttcagcccc aggttacggc gtttacgttc actacctgcc cgtgccgcag cggcggaatc
    30721 gtggaatggc gggttacaca aggtcgcatc gtattgctcg tttttatgga tgatgccgtt
    30781 aaaaatcgcc ccactctctt tttgccgacg cagacgaatg gcgcggttaa gccccggatt
    30841 agcactgatg atcgcctgcg cactgcttaa cgcctggctg ctggtttcac taccggtaaa
    30901 acgccagcca tattcatgta cgccaatcag cggataaata cagttcgcac caacaccgat
    30961 atccagaatg ctggcattag ctggaattgt tccgcttgct tcagccagta aatcggcaag
    31021 gtgatgaata taatccgccc ggcccggtac tggcgggcag agaaaaccgt ccgggatatc
    31081 ccagttcgct acggcgtaaa aatgggccag caacgcctta ttgagcgcct tcaccgccag
    31141 cggattggca aagtctacgc tttgctcccc ggcgggtgta agcgtgagga attgcctgag
    31201 ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac ggttgcgcgg
    31261 atgcaacccc ggtttctggg cggacatagc attctccttt aagcagcggc gtaagatacc
    31321 cgcataacgc gacagggtaa atatctggct gcgtttatca tttctaaaaa ccttcacatg
    31381 gctatggttt atggtaattc accataaaaa tca
//
LOCUS       DAZKJR010000049        31195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKJR010000049 DAZKJR010000000
VERSION     DAZKJR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.049"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            428..517
                     /gene="tisB"
                     /locus_tag="ACHOWS_002240"
     CDS             428..517
                     /gene="tisB"
                     /locus_tag="ACHOWS_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFQ0047806.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(641..715)
                     /gene="ysdE"
                     /locus_tag="ACHOWS_002241"
     CDS             complement(641..715)
                     /gene="ysdE"
                     /locus_tag="ACHOWS_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFQ0047807.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            797..1981
                     /gene="emrD"
                     /locus_tag="ACHOWS_002242"
     CDS             797..1981
                     /gene="emrD"
                     /locus_tag="ACHOWS_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFQ0047808.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(1989..2486)
                     /gene="yidF"
                     /locus_tag="ACHOWS_002243"
     CDS             complement(1989..2486)
                     /gene="yidF"
                     /locus_tag="ACHOWS_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0047809.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(2483..2845)
                     /gene="yidG"
                     /locus_tag="ACHOWS_002244"
     CDS             complement(2483..2845)
                     /gene="yidG"
                     /locus_tag="ACHOWS_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFQ0047810.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(2835..3182)
                     /gene="yidH"
                     /locus_tag="ACHOWS_002245"
     CDS             complement(2835..3182)
                     /gene="yidH"
                     /locus_tag="ACHOWS_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFQ0047811.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(3242..4735)
                     /gene="yidJ"
                     /locus_tag="ACHOWS_002246"
     CDS             complement(3242..4735)
                     /gene="yidJ"
                     /locus_tag="ACHOWS_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFQ0047812.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGDNILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(4732..6447)
                     /gene="yidK"
                     /locus_tag="ACHOWS_002247"
     CDS             complement(4732..6447)
                     /gene="yidK"
                     /locus_tag="ACHOWS_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFQ0047813.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            6614..7480
                     /gene="yidL"
                     /locus_tag="ACHOWS_002248"
     CDS             6614..7480
                     /gene="yidL"
                     /locus_tag="ACHOWS_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0047814.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(7570..9231)
                     /gene="yidE"
                     /locus_tag="ACHOWS_002249"
     CDS             complement(7570..9231)
                     /gene="yidE"
                     /locus_tag="ACHOWS_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFQ0047815.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(9427..9855)
                     /gene="ibpB"
                     /locus_tag="ACHOWS_002250"
     CDS             complement(9427..9855)
                     /gene="ibpB"
                     /locus_tag="ACHOWS_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFQ0047816.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(9967..10380)
                     /gene="ibpA"
                     /locus_tag="ACHOWS_002251"
     CDS             complement(9967..10380)
                     /gene="ibpA"
                     /locus_tag="ACHOWS_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFQ0047817.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(10374..10446)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            10685..11017
                     /gene="yidQ"
                     /locus_tag="ACHOWS_002252"
     CDS             10685..11017
                     /gene="yidQ"
                     /locus_tag="ACHOWS_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFQ0047818.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(11019..12233)
                     /gene="yidR"
                     /locus_tag="ACHOWS_002253"
     CDS             complement(11019..12233)
                     /gene="yidR"
                     /locus_tag="ACHOWS_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFQ0047819.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDSTLDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            12334..13398
                     /gene="cbrA"
                     /locus_tag="ACHOWS_002254"
     CDS             12334..13398
                     /gene="cbrA"
                     /locus_tag="ACHOWS_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFQ0047820.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTMLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(13395..14732)
                     /gene="dgoT"
                     /locus_tag="ACHOWS_002255"
     CDS             complement(13395..14732)
                     /gene="dgoT"
                     /locus_tag="ACHOWS_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0047821.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(14807..15955)
                     /gene="dgoD"
                     /locus_tag="ACHOWS_002256"
     CDS             complement(14807..15955)
                     /gene="dgoD"
                     /locus_tag="ACHOWS_002256"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFQ0047822.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(15952..16569)
                     /gene="dgoA"
                     /locus_tag="ACHOWS_002257"
     CDS             complement(15952..16569)
                     /gene="dgoA"
                     /locus_tag="ACHOWS_002257"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFQ0047823.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMSCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(16553..17431)
                     /gene="dgoK"
                     /locus_tag="ACHOWS_002258"
     CDS             complement(16553..17431)
                     /gene="dgoK"
                     /locus_tag="ACHOWS_002258"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFQ0047824.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGFNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(17428..18117)
                     /gene="dgoR"
                     /locus_tag="ACHOWS_002259"
     CDS             complement(17428..18117)
                     /gene="dgoR"
                     /locus_tag="ACHOWS_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFQ0047825.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            18394..19050
                     /locus_tag="ACHOWS_002260"
     CDS             18394..19050
                     /locus_tag="ACHOWS_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047826.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(19094..19906)
                     /gene="yidA"
                     /locus_tag="ACHOWS_002261"
     CDS             complement(19094..19906)
                     /gene="yidA"
                     /locus_tag="ACHOWS_002261"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0047827.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTTLSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(20021..20419)
                     /gene="yidB"
                     /locus_tag="ACHOWS_002262"
     CDS             complement(20021..20419)
                     /gene="yidB"
                     /locus_tag="ACHOWS_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFQ0047828.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            20537..20638
                     /locus_tag="ACHOWS_002263"
                     /pseudo
     CDS             20537..20638
                     /locus_tag="ACHOWS_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20659..23073)
                     /gene="gyrB"
                     /locus_tag="ACHOWS_002264"
     CDS             complement(20659..23073)
                     /gene="gyrB"
                     /locus_tag="ACHOWS_002264"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0047829.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(23102..24175)
                     /gene="recF"
                     /locus_tag="ACHOWS_002265"
     CDS             complement(23102..24175)
                     /gene="recF"
                     /locus_tag="ACHOWS_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0047830.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(24175..25275)
                     /gene="dnaN"
                     /locus_tag="ACHOWS_002266"
     CDS             complement(24175..25275)
                     /gene="dnaN"
                     /locus_tag="ACHOWS_002266"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0047831.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(25280..26683)
                     /gene="dnaA"
                     /locus_tag="ACHOWS_002267"
     CDS             complement(25280..26683)
                     /gene="dnaA"
                     /locus_tag="ACHOWS_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0047832.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            26980..27060
                     /locus_tag="ACHOWS_002268"
     CDS             26980..27060
                     /locus_tag="ACHOWS_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047833.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            27290..27430
                     /gene="rpmH"
                     /locus_tag="ACHOWS_002269"
     CDS             27290..27430
                     /gene="rpmH"
                     /locus_tag="ACHOWS_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0047834.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            27447..27806
                     /gene="rnpA"
                     /locus_tag="ACHOWS_002270"
     CDS             27447..27806
                     /gene="rnpA"
                     /locus_tag="ACHOWS_002270"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0047835.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            27770..28027
                     /gene="yidD"
                     /locus_tag="ACHOWS_002271"
     CDS             27770..28027
                     /gene="yidD"
                     /locus_tag="ACHOWS_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0047836.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            28030..29676
                     /gene="yidC"
                     /locus_tag="ACHOWS_002272"
     CDS             28030..29676
                     /gene="yidC"
                     /locus_tag="ACHOWS_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0047837.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            29782..31146
                     /gene="mnmE"
                     /locus_tag="ACHOWS_002273"
     CDS             29782..31146
                     /gene="mnmE"
                     /locus_tag="ACHOWS_002273"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0047838.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         7463 a         8361 c         8003 g         7368 t
ORIGIN      
        1 cccgccggag cgaggtttcg tcagtcgcct gcggctggta accgcaaagc acactgtatt
       61 atgtcaacac tgaaagtata cgtgttccgc gcagaacgcg caatttcggc acgaattttg
      121 acgtatttag tgcatagttg agtatcgatc acagtttgcg ttttgtccaa aattactgtt
      181 tatttataca gtaaacttct ataatatcac tgtacgcaat gtgttatgcg ggggccgcat
      241 cgttacccgg cgcactaagt cctggcagaa acgggtggtg ccgtcagcgc cttaaccccg
      301 cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac
      361 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
      421 gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
      481 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcactaaag
      541 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggtgtcg gatgcgatgc
      601 tgacgcatct tatccgccct accatctctc ccggcaacat ttattgccgc ttttgtttac
      661 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
      721 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
      781 aaaagcatcc gtgataatga aaaggcatag aaacgtcaat ttgttattga tgttggtatt
      841 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
      901 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
      961 ttacggtgtc tcacagctgt tttatggccc gatttccgac cgcgtgggtc gccgaccggt
     1021 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag
     1081 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
     1141 aatggcgcgt actttgccgc gcgatttata tgaacggaca cagttgcgcc atgctaacag
     1201 cctgttaaac atggggattc ttgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
     1261 ggatacgatg tggaactggc gtgcctgtta tctctttttg ttggttcttt gtgccggtgt
     1321 gactttcagt atggcccgct ggatgccgga aacgcgtccg gtcgacgcac cgcgcacgcg
     1381 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
     1441 gctgattggc ggtctggccg gaattgccgc ctttgaagcc tgctccggcg tgctgatggg
     1501 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
     1561 ggcatttttt ggcgcatggt ttgccggacg tcccaataaa cgcttctcaa cgttgatgtg
     1621 gcagtcggtt atctgttgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
     1681 cgtgatgaat gtctggacgc tgctcattcc cgccgcgctg ttctttttcg gtgccgggat
     1741 gctgtttcca ctggctacca gcggcgcgat ggagccgttc cccttcctgg cgggcaccgc
     1801 tggcgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
     1861 gatgttgccg caaaccggtc agggcagcct gggattgttg atgaccttaa tgggattgtt
     1921 gatcgtgctg tgctggctac cgctggcgac gcggatgtcg catcaggggc agcctgttta
     1981 agctcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
     2041 tcacgcatca cccgcatatg cggcgcggag taagaataaa acgcctgata gcccgcacaa
     2101 agcacgttgt ctggtttatc ttcccgagcg tgcgggcaca gccgatgcca gtcgcacgcc
     2161 tggcagtcac ggcgcggttg cgacttttcg ctgccacacc atttttgtaa ggtggtgtca
     2221 aaaagttcaa tcgaaatacg actgatatct gcccgcaccc aacactcgaa cagggcaatt
     2281 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
     2341 aattcaagat ggggaataag ctgtactgag cggatgccca gctgtttgaa attgtgataa
     2401 aaatcggcag gttgcagagc atccttatac tctacaacca gtttatgcct gtcctcttca
     2461 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
     2521 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
     2581 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
     2641 gggtgtagtg ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata
     2701 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
     2761 gcatggtacg aaaccacgcc agtgatgtgc gttccggctg cagccctggg tcggcaatgc
     2821 gtctggcttt gcggctatcc ggcatacaac accagtccca tgacgatcac tgccacgacc
     2881 atcaatatta aactgatgat taataagctg ttggtatagg gcaggtcctc tttcaggcgc
     2941 atcgcttttt cattgcgtaa ccagcgcaag tagccataca tcgctaaccc gccggaaaac
     3001 agacacaaca acaacgccag cagttcgcgg atgacgggcg tggcgaaatc tggcgcaagt
     3061 tgatcaagcc caacccccgc cgccagaaat cctagtgcag tacggatcca cgccagaaaa
     3121 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccgaggcg ggaaattttc
     3181 attgttgctc cttgtaaact gttcctcgcc gaaaaattag gccaggaaca gcaataatgc
     3241 atcagaactt ctgttttttc tcttccacct tcaccccttg tgtcggtaaa ccggtgtcat
     3301 agtcgcgtac cactggagaa taaccatcct gtgggcgtgg acgaaacgcc cccatccagc
     3361 gcggttgtgc atctttgcgc cacggacgca ggttccattg ataactgcgg aacggatcgc
     3421 gaattttgtc catataatcc aataaggcgt catgcatttt gctgcgaacg tcggcaaaat
     3481 ggatatcatc gatcaggtta tgcatttcat ttgggtcatt acggcgatcg taaagttcat
     3541 cactggtgaa gaggttgagc accagtttaa agtcatccgt tacccagcaa cgcaccggaa
     3601 taaaaccgcc aaagctgtca tgctcaatct cgtagcgatt aaattccacc aacacgccac
     3661 gtggttcttt tacggcaagg atattatccc ccggcagaat ctctggtttt tcaatatctg
     3721 ccagcgccat cattgtcggc agtaagtcaa tatgactgac tggcgtatcg acctgtcgcc
     3781 gctccccttg cggcgaacgg atgatcagcg gaatgcgggt gatgtcgtca tacatcgccg
     3841 cccctttact gatcagctta tgtgcgccca tcatttcacc atgatcggag gtataaatga
     3901 cccacgtatt ttcacgttgc tctggcgtta aggcattgat gacacgtccg atttggtcat
     3961 caacaaagtc attgcaggca aaatagagcg gatggtgata aagcccgtca tcaccgactg
     4021 gcgatggcat cgcctgcgcc cataagcggt gatgttccgg tttgttcgcc aggtcatcct
     4081 gtgatttctc gccaagttcg tagtaaaaat cagcgtattt ctctaaatac tccaccggac
     4141 aggtgaacgg gtgatgcggc tcatcatacg aaaccaccat caggaaaggt tcgtcggcgc
     4201 gcgcgggctg ttgcagaaaa tctaccgccc gattgctgat gcggtgcgcc caggtgaagg
     4261 tttcgtcgat atggttcgcc tgtaaatcct caacgctgtt taggccattg cgccacaggc
     4321 tgatctcttt ttccgtcagt tcgctaaggt agttcgcccc atcgaaccag taatcagcgt
     4381 cccactccgg cggacactcg ccagtgccga aatagtcatg accgtcgaga tgccatttgc
     4441 caatgtaaca ggtgtgatag cccgcatcct taaagtagcg ccccatggtg gaaatgtttt
     4501 tacctggcgc gacgttgttg gtccacgggc cggactggtt agcgtagata ccggtaaaca
     4561 gcccggcgcg cgctggtgta caaaccggtg aacaggtata ggcggaatta aagcgaatac
     4621 tttccgccgc cagactatca atattttgcg tattcagcgg cttaccgcta tagcaaccga
     4681 ccatattggt ggcctgggta tcggtcatga tgaacagaaa attggggcgt ttcataggct
     4741 atccttcgcg tcgggagtaa aggttacggc tgggtcgtga cgatgccgcc agctatcaaa
     4801 aatcaggtag acaatcacta cggcggcaat gaaataactg atcatcgcta accagcgcgt
     4861 accgtagccg ccgaattcag ccagcccggc atagacgcca atcatcgcga acaggatgcc
     4921 cattgacgcg atcttgacgt ttttccacgg tttcatgtcc accgcaaacg catctttgaa
     4981 ggtgaacggc gtggcgcgcg gtttgataaa accgatcacc agcatcacga ccacgttgat
     5041 gcagaacgta caggccagca catagaggaa atggaagtcg aacttcacca gatagttgat
     5101 ggtgatgtag ctgattatgc caatccccat cgccactttt gccgccagcg ccgggatgcg
     5161 cgggaagaaa aagcccataa tgatgatggt aaccagcggc acgttgtaaa tgccgttgag
     5221 ctgtttcatc cagctataca gcccctgcgg cgcgttggcg atccacggcg ctaccagcac
     5281 cgaaacgatg gcgataaaga aaccaaattt gcgcccgacg gtgaccagct gctgcggctc
     5341 ggcattctgg ttaatgatgc gccggtaaat acccatactg aataacgtac tggcgctatt
     5401 cagaaagccg ttgaaggtac tgatcaccgc accaaataac accgcgccga agaaacccac
     5461 cagtggcact ggcagaacgt tattgaccag cgtcgggtag gccatgtcgg ctttcggtag
     5521 atcctgatac agatgaaacg caatcaaccc tggcagcacc agtaccagcg ggtcgagcat
     5581 tttcagcacc gccgttaaca gcgccccctt ttgcccttcc gccaggcttt ttgacgccag
     5641 cgtgcgttgc acgatgccct gatttgtaca ccagtaaaag gtgttcacca gaatcaaacc
     5701 ggtaaatgcc gcgccaatcg gcaagggatc ggtcgggccg ccgactgagt ttaatttctc
     5761 ggcgtgaacg gtggtgagtt gctcaatgcc ctgcataaag ctgcccttgc ccatcgcgat
     5821 caggccaaat accggcacca tcaacccgcc aataaccagc ccaataccgt taatggagtc
     5881 cgccactgcc attgcgcgca gtccgccgat caccgcatac aaaattcccg ccagaccaag
     5941 caaaataacc agtaaccaga tagccgcacc atcggaaagt tgtagcgact cacccacatg
     6001 aaacaggctg ttgagcgcca acgcgccgga atagagaaca atcggcagaa agcagacgcc
     6061 ggtggcaatg aggaagcaga agtcgatgat aatacgcgtc gttttatcat aacgttcctc
     6121 cagaaaatcg gggatggtgg caatgccgcg cttcagatag cgcggtaaaa agattagcgc
     6181 gaggaagatc agcgtcactg cagaggtgac ttcccagccc atcaccgaca tgccactttt
     6241 gtaggcctgc ccagaaaggc cgaccagttg ttccgtggaa aggttggtta gcattaacga
     6301 agcggcaata accggcgctt ttagtgaacg tccggcaaga aaatagcctt gttgcgatcc
     6361 ggtatctgtt ttacggacct tccaccaggt gatgaccgct accagcagcg taaaaccgac
     6421 aaaactcaag atttgtaacg aattcatctc atagccctta tttatgttta tgtcccggac
     6481 gcatgaatat agggagaggt aaaatgcgtc gggtcaggca gtcagattct taaaatacaa
     6541 acgtcgtatc cctgaacgga ttgttttctg ttaagttcag gttgtgagca taattcgccg
     6601 gagggatatt aaaatgaatg gaaaattgca aagttcggat gtaaaaaacg aaactccgta
     6661 caatattccc ttattgatta acgaaaatgt gatctccagc ggaatttctc tgatatcgct
     6721 ctggcatacc tacgccgacg agcattatcg ggtgatctgg ccgcgggaca agaagaaacc
     6781 gcttatcgcc aactcatggg ttgcggtgta taccgtacaa ggatgtggga aaattctttt
     6841 aaagaatgac gaacaaataa ctctgcatgg caactgtatt atatttttaa agccaatgga
     6901 tattcactct tatcactgtg aaggtttggt ctgggaacag tattggatgg aatttacccc
     6961 caccagtatg atggatattc ccgtcggtca gcaaagcgtt atttataatg gcgaagttta
     7021 taatcaggaa ctcaccgaag ttgctgagtt aattacttca ccagaagcaa taaaaaataa
     7081 tctggcagtc gcttttctga cgaaaattat ttatcagtgg atttgtctta tgcacgcagt
     7141 cggtaaaaaa gatccacaac ggcggcaaat tgaaaaatta attgccactt tacatgccag
     7201 tctgcaacaa cgctggagcg tagctgatat ggctgccacg atcccctgta gcgaggcctg
     7261 gttgcgtcgt ctgtttttac gctataccgg caagacgccg aaagaatatt acctcgatgc
     7321 gcgtctggat ctggcgctat cgctattaaa gcaacaaggg aactcggttg gcgaagtcgc
     7381 tgatacgctc aacttcttcg actcctttca tttcagcaaa gcctttaagc ataaatttgg
     7441 ttatgcgccg tcagctgtgc tgaagaatac ggacaggtaa taaccagaat ggcgagtgtg
     7501 taaatgactc gatgatgttc agaaatacca ggtggagtac catttacggc gactccaccc
     7561 ggaaaagagt taaccgatac tccagaagag caccgccagt aattgggggg tgataatgcg
     7621 caggaacatc accaacggat agacagtggc gtaagagagc gccgccgcac cgctcgttgg
     7681 atgaagatta ttagcaaatg ccagcgccgg aggatcggtc atggagccag ccagcatccc
     7741 gcacatggtc aggtaattca ttttggctaa catccgcgcc agaatgccaa cagtaatcag
     7801 cggaacggcg gtgatcaggg caccataacc aatccagctt agcccttcgc catccaccag
     7861 tgtatggata aaatcgccgc cggatttcag gccaaccacc gagagaaaga gcacaatgcc
     7921 taactcgcgt agcgccaggt tagcgcttgg tggcataaac cagtacagct tgccgatact
     7981 gccgatacgc ccgaggatca acgccataat cagcggaccg cccgccagcc cgagtttcaa
     8041 cgccgccggg aacccaggca caaagacggg aatagaacct aacaatacgc caagcccgat
     8101 accaataaac actggcagca tctgaacctg ttgcagtttt tgctgcgcat tccccagcac
     8161 attggcaacg gcatcaattg cggacggacg ccccaccagg ttgaggatat cgccgaactg
     8221 caggctgata tcgctacttg caactaattc aacaccagca cggttcaggc gcgagataac
     8281 aacgtcatag cgttctttaa agtgcaggtc gcgaatacgt ttaccgagca cgttttcatt
     8341 ggtgaccacc acacgctcga cgcgcaagtc agtgcctttc gtagacagcg aggtatcgac
     8401 ctcctgacca atcaccagtt gcgcattatg taaatccgca ggctgcccca ccagatgcag
     8461 caaatcgccc agttgaataa cggtttctgg cgaaggcact tttagggttt cttctcgttt
     8521 caatcgtgag caaataactt tatcgccatt gagaatcggc acatctttaa tggctaaatt
     8581 atgcaaatta ggattctcaa ctcggatgtt aatcgtcctg attagcgcac cgccgttggt
     8641 tcgcgtggac tcgtgctgct gggcctcagt ctcaacattg acgcggaaaa taacccgcag
     8701 catccacata gtgaacaaaa tgccgcaaat cccaaatgga tacgccatcg catagctcat
     8761 ccccatttga tcgaccatcg ccatcggtgt ccccaggtcg cgcaagatct gctgccctgc
     8821 ccccagtgct ggcgtattgg ttaccgcacc ggagaaaatc cccagtacta ccggcagtgg
     8881 aatatcaaac agtttatgca ggatggcggt aaccagcccc ccgatgatga cgatcagaac
     8941 agcaaacagg ttgaggcgta atccggagac gcgcaatgag gcaaagaagc ccggccctac
     9001 ctgaatcccg atggtataaa cgaacaggat caggccaaat tcctgaataa catgcagcat
     9061 atcgctactt aatgtcatcc ccgcctgaga aacaaaatgg ccgacgatga tcccaccgaa
     9121 cagcacgccg ccaatgccca atccaacacc gcgaaacttg acgttgccga taaacaaccc
     9181 gacgaccgcc accaaagcca gaatactgac cgttaatgct atatcactca tcgtccattt
     9241 ctcttgagat aacattttaa ttgctacgga ttatggctga atcggtgaag cagatttgcc
     9301 ttatagcgca cattatcacg ggaaagaacg tgccgaaata tcttaaacag gcgaaaatta
     9361 acgcattgtt gaagaagaaa gacaaaaaaa gccccaccag aatggcgggg caaagagaat
     9421 agctagttag ctatttaacg cgggacgttc gctgatagcg atacgctgcg ctgcgatggg
     9481 ttcaggctca ttacgaatta aatcaatatg cagtaaaccg tttacgaagg ttgcaccaga
     9541 gacttccata ttttcagcca gcgtaaagct caggctaaat ggctgattca taagcccttg
     9601 atgcagccat tttttctctt cttttggctg ctccggcgtg ccttttacgc tcaggcgcgt
     9661 accttccagt tgaatctcta aatcttcctg acgaaaacct gccagcgcaa gggtaatgcg
     9721 gtagtggtta tcgtcgcttt tctcaatgtt gtacggcggg aagctctggc tttcaccggc
     9781 gttttgcagt gcgttggcca gtttgtcaaa accgatccat tgacgcatca gtggggataa
     9841 atcgaagtta cgcatagtca tttctccttc taagaagcga gtaagtacct gcaaatccga
     9901 agattcgcat atgctccctg acggcgagca tggagatgtc aggccgcgca aggcggcctt
     9961 agggaattag ttgatttcga tacggcgcgg ttttttcgct tccggaatca cgcgttcgag
    10021 atcgatatac agcaaaccat ttaccaggtt agcaccacga acatgaatgt tctcagctaa
    10081 ctggaatttg cgttcaaagt tgcgttcagc gatgccctgg tacagatagg tgcgctcttt
    10141 ttgttcgtcg gcgtgagcac ctttcaccac cagcagatta tcctgggcgg taatttccag
    10201 ttcgctctca gcaaaaccag ccacagcgat agcaatgcgg taatggtttt cgtctaccag
    10261 ttcaacgtta tacggagggt agccgccatt actctggctc tggttgtttt ctaagtggtt
    10321 aaacaaacgg tcaaatccaa tagcagaacg gtaaagcggg gataaatcaa agttacgcat
    10381 aatcaatagc tcctgaaatc agcgagaatc taagaccttc cacaatggac aggtcaggta
    10441 gccagaacac ccatcaggcg cgtcctcatc ggctacgatg taaaaatggg tctggaaatg
    10501 aacttttcaa gccttatcag cggacttttt ttgcagttta tggtctattg catagactga
    10561 gggggcagca cgaaacgtta aagtgcgata gccgccacag tgcgacgtaa tgatgatgtt
    10621 attttcagca agggatcgct ataactcatc aggccaacac ccacgatcaa cagatgaatt
    10681 tattatgata agaaatgtgt tgttagcgtt catgatatgc agcggaatga cattacttgg
    10741 aggatgctcc agcgtgatgt cccataccgg ggggaaagaa ggaacgtatc ccggcacccg
    10801 cgccagcgct accatgattg gcgacgatga aaccaactgg ggcactaagt cgctggcgat
    10861 tctcgatatg ccgtttaccg ctgtgatgga cacgctttta ctgccgtggg acgtgttccg
    10921 caaagacagc tcggtgcgct cacgcgttga aaaaagcgaa gctaacgcgc aagccaccaa
    10981 tgcggttatt ccacccgcca gaatgcctga taactgattt agcgatcagt ttcggtgatc
    11041 cacagctggc cgccttccat ccacgccagc cactgaccat ccggcgagaa gaccacggcg
    11101 tccgcagaag gtggattggc gtggttttcg gttaaatact caacctcgcc gctttgcgca
    11161 tgggcgcaag caattcgatt atccagcaca aagcccaacc attctcctga tggatgccag
    11221 ttaaatgcag actgaatatc cgttttgtta tgggttaact ggcgcggctc gccgccctgt
    11281 ggcgagataa gccacagttg cacaatgccg ttatcatcac gcattaaaaa cgcgatttgc
    11341 gtaccctgcg gattacagcg cacccagtgg cgcgggacgt tgactaaccc cggataagcc
    11401 cgatggtggg taaaggttaa acgtcgctgc acgacgccac gcggtggcgc gggcagggtt
    11461 gtttccgttc cacttaacgg cgcatcacca gctgctttcc agccagcttc atcttgcggt
    11521 aactcaacga taaatagctc cggcactttc tcgccctttg gcgaaagtgt gtcgccaata
    11581 aacgccagcg cgtgatttcc tacccatcct tcttcataag cacgattgat ttcattactg
    11641 ccaggctgcg gcgtgggcgt tgttttactc accagcacgc accagtggct accgctgtat
    11701 tcacgcggat gctgcttttg tacgttgacc gggccaaacg gcgcagcaac acctacgttt
    11761 cgcaaatcca gcgtcgaatc gagttgatgc attacatggt cgttataggt aaagctcacc
    11821 ctttctccgt tcgggctaaa gacatgcaca tggctgccgc cgcgcagcgc tcctggggtg
    11881 tacggcgcgg taatatccat tgcatcgaga ttgctcacct tgccgccttc agcaatcact
    11941 ccgcgccgat gatggaagtc gtaataccat gtttcatcag gattttccgg gccgtggatg
    12001 aaaacatatt tctctgactt cggatgaacg gtcaccacgc cgacgtacgc gccctgtgac
    12061 gcgcgataga taacctcgac ctcgccggta tggatattca cacgctcaat ggtctcgccg
    12121 gtaaacgacg cccctgaagg acgcacgtca aataccagcc actggctatc gggcgtccag
    12181 gtattggtat tggtgagcag gtgattacgg ggagcaaagg ttatctgttt catggcgcgg
    12241 tcctgataca aaacgggtcc cgccatcata cttcacaagg acttcacttt cagacaatag
    12301 tcttttgcca tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt ggcgattatt
    12361 ggcctcggcc cggcagggtc ggcgttggcg cgaaagttag caggcaaaat gcaggtgatc
    12421 gcgctggata aaaagcacca gcatggtact gaaggtttca gcaaaccttg tggcggtctg
    12481 ctggcaccgg acgcgcagcg atcttttatt cgcgatggac tgacgcttcc tgtcgatgtc
    12541 atcgccaacc cgcagatttt cagcgtcaaa accgtcgacg tcgccgcatc gctcacgcgt
    12601 aactaccagc gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg
    12661 ctgatccccg ccagcgttga ggtttaccac gacagcctgt gtcgaaaaat ctggcgtgag
    12721 gatgataaat ggcatgtcat ttttcgcgca gacggctggg agcagcatat taccgcccgc
    12781 tatctggtcg gtgccgatgg tgccaactcg atggtgcggc gacatctcta cccggatcat
    12841 caaattcgta aatatgtcgc tatccagcag tggttcgcag agaaacatcc ggtgccgttc
    12901 tactcctgca tctttgataa tgcgataact gactgttatt catggagtat cagcaaagac
    12961 ggttatttta tctttggcgg tgcctatccc atgaaagacg gtcagacgcg tttcacgatg
    13021 ctgaaagaga aaatgagcgc ctttcagttc cagtttggta aggcggtgaa aagcgaaaaa
    13081 tgcacggtgc tgtttccctc acgctggcag gattttgtct gcggtaagga caatgccttt
    13141 ctgattggtg aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg
    13201 ctggatagcg cagagattct gcgttcggtg ttactgaagc agccggagaa gagcaacgcc
    13261 gcctactggc gcgccacccg caaactgcgt ttaaaactct tcggcaagat agtaaaaagc
    13321 cgatgcctga ccgcaccggc tttaagaaag tggattatgc gcagtggtgt ggcgcatatt
    13381 ccacagttga aagattagcc aacgcgcttc acatcgccca ccagcaggat gtaagagagc
    13441 gcgccaatca acgcgacggc ggagatataa accagtgcag gtgcgaaacc gtaaccctgc
    13501 gccaggtacc ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac
    13561 acgccgccgg ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggta
    13621 atcgaagcaa aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga
    13681 tcgttggtgt agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc
    13741 gttttacgcg caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca
    13801 gagagcagga cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc
    13861 agcgccgtga ttcctttttc ctgggttaaa tagttcggga accaagttaa gaaaaaccac
    13921 agtgtagaag ccaccgcaaa ttgcccaaga tagacgccga tcagtttacg atggaacacc
    13981 agtttccagt ctttggctgt taacggctga cgcgcctctt ttttcaccgg cgcatcacca
    14041 tccaccagac cgccgccatc acgaatgtaa tccagttcag ctttgctgat gcctttagtc
    14101 aggcgcggcg gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccaccg
    14161 gtgacaatga acacccagtg ccagctcaac atttcctgaa tccagatcag cagcggagtc
    14221 aaaaacgcca gaccgacaaa ctgaccagac gtataaaaac caacggcgga agcgcgttca
    14281 tgttccggga accagctggt caccatccgg ttattggtcg ggaaagcggg cgcttcgaaa
    14341 ataccggtta ttgcgcgcag accaattaat gacatcaatc ccgtggcaaa gccctggaat
    14401 aaagtcgcca ctgaccagcc aaatatcgca ataaaataag tcacgcgtga acctacgcga
    14461 tctaaaaacc aaccgccggg gatctgacat agtgtataaa gccaggcgaa ggccgaaaat
    14521 acatagccca tttccgcttt ggtaataccg aactcttcct gaatatgggc ggaagccacg
    14581 gccaggttag cgcggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc
    14641 agataacgcc gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc
    14701 cagattttgg gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt
    14761 ttgtattgct gttttagtgc ccgatgagag gcttacgtgg caggaattac cactctgcta
    14821 cgctgttatc ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact
    14881 caataacttt agcttcgtcg atttccacac ctaagcccgg tttcgttaat ggtttaaaga
    14941 aaccgccgac catgctgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt
    15001 tgttgtaatg aatgcccata ctttgttcct gaagtacggc gttataggaa acaaagtcga
    15061 tatgcaggca agccgccagt gcaatcggtc cgagcggaca gtgcggcgca agggtcacgt
    15121 cataggcttc tgccattccg gcaattttgt agcattcggt aataccgccc gcgtgggaga
    15181 gatctggttg cagaatcgaa ataccacccg cctccagcac acgtttaaaa tcgaaacgtg
    15241 agaacatgcg ttcacccgcc gccagtggaa tatgcgtttg tgccgccagt ttcgggtagt
    15301 attcggcttg ttctgccagc accggctctt caataaacag cgggcgatac ggctccagtt
    15361 ctttaatcag cactttcgcc atcggcgcgc tgacgcgacc gtggaaatca agaccaaact
    15421 caatctgatt gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg
    15481 cgcgggagtt atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc
    15541 cgatttcgcg cagggtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc
    15601 aactgtaggc tttaattttg tcgcgaacca ggccgcccat cagttgccag accggcgcat
    15661 tcagcacttt gcctttgata tcccataacg cctggtcgat cccggcgatg gcgctcatca
    15721 ggattggacc gccacgataa aatccggcgc gatacatcac ttgccataag tcattgatgc
    15781 gcgatggatc ctgaccaatc aaatagtcac ccagctcgtg aactgccgct tccaccgtgc
    15841 gggcgcggcc ttcaatcact ggctcgcccc agccgaccac gccttcatcg gtttcaattt
    15901 tcaggaacat ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac
    15961 tgcctctcga tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt ctacggactg
    16021 cccggcgcga tagagatcgc tgcctaagcc cgcccctgca caacctgcgt ctatccactg
    16081 cgccaggttt tctggcgtca cgccgccaac ggcaaagact gcgatgtccg gtggtaatac
    16141 cgcttttaac gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagcgc
    16201 ctgagcgccc gcgtcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcaaac
    16261 ggtcatgccg tagcccaccg cacggcggat cacttcacta tggatattgg gcgtaacgat
    16321 gagctggcag ctcatcctgg cgagtgcatc gacctgttca ggtttcagta ccgtacctgc
    16381 gccaatcaac gccttatcgc catacgcatc aacgatggcg ggaatgcttt tctcccattg
    16441 aggggaattc agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg
    16501 cgccagcgcc tcgtcgggcg taatgccgcg caaaatggcg atcagcggga gtttagtttg
    16561 ccactgcatg agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg
    16621 tcacgtcgca acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg
    16681 cgacaagggt gatggcgtgt tgatgggtca catagtcgcg catactggca acctctgcgc
    16741 caatcaacaa accagagaga aattcgctga cctgttcgcg gggaagtgtt cccagcacat
    16801 gcgaggcgcg aacttcaaaa agctgcggca atatggcggg agcattaaag ccacgctcaa
    16861 gaccagccgc gaaggcatcg gcagagtttt cctgcggcgg caaacctgcg ccaatcaatg
    16921 agtgatttaa cagtaaatga tgtaattcac cggtcatcac ggtgcgaaaa tcgttgattt
    16981 gctggctatc ggcctgcacc catttgcaat gggtaccggg cataacataa agagaggaag
    17041 gagcgagagc acgcgcgccg atcaattgtg tttcttcgcc gcgcatcaca ttgtggttat
    17101 cgtcatgaga gacgcataat ccgggaataa tccagatatt gtcgccaacc gacgttaatt
    17161 gatcgccaat agacgaaaaa cgggcaggaa cagataaata cggtgcaact ttccagccga
    17221 cattgctgcc gaccattcct gccatcacca ctggcgtatt ctcttcacgc cagtcggtcg
    17281 tgacttctgc taacaccgca gccggagatt ttccgttcag gcgcgtgacg cctgcttctg
    17341 attgcctgct gtccaggcag tggtcgccct gataaagcca ggcgcgcaga ttggtcgatc
    17401 cccagtcaat tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc gtgtcgagct
    17461 ggcgatcatg gtaagcgccg cctgctctgc cgcatcgccg tcctgatgcc gtattgcatc
    17521 gaacagcgcc ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc ccatccaggt
    17581 tcgttcaaaa accgcccgct gcagcgaact gatcgcaatg ctaagttgct gtaacaccgg
    17641 gttatgcacc gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt taaacgcttc
    17701 gcggtcctga ttgttggcaa tcatctcgtt cagcgccgat tcaatctgcg ccagatcgct
    17761 ggaggtcgcg cgctctgctg cccaacgggc aatagccggc tctaccaggt ttcgcacttc
    17821 gctcattgca ctgataagcc gtgggtcata gtcgttttcc agcacccatt gcagtacgtc
    17881 agtgtcgagg taattccact ggttacgcgg agccacaaac gccccgcgat aacgtttcat
    17941 ttcaatcagc cgcttcgcca tcagcgaacg aaacacctca cggatgatgt tgcgcgaggt
    18001 tgcaaactct tcacagagct ccgcctcagc cggaagtgga gagcctggta cgtatttgcc
    18061 gtgaacgatc tgtttaccca gcgtaataac gatgcgatcg gttttattga gagtcatgga
    18121 gagtccttgt gctctgtatg ttcttctcta ctttaccccg atcgatgcat aacgcggcaa
    18181 ctttgtagta ccagcgtgat gacgttcgcg tttgctgtgc gcctaatgta gtacaaactt
    18241 atattgttgt actacaattt agatcacaaa aagaacaata cataaaaaat gacatgcgtc
    18301 gggcagaaat ctgaaaaggg atgtcaggcg ctaaacagga gggaaagaag agtatgcttt
    18361 caacggctta gctactcgtt taaaggatta atcatgaagt tgaattttaa gggatttttt
    18421 aaggctgccg gtttgtgttc tttggcattt gcgttgaccg gatgtatcac ctggggattg
    18481 gtttccaata ctgcaagccg tccacatgcc gagcaatggc ggtcagacac gattaagggt
    18541 ttatcgctgg ctgaagacag taacggtacg aaaggctatg tttttgttgg tgaatcgttg
    18601 gactatctgt tgaccaccgg cggcgatgag gtggttaaaa tgctgaacga tccggcaatc
    18661 catggtgaaa gaatcaccgt ttctgataac gcgaaattta ttttaagcag cagtaataag
    18721 aatttctctg gtgctattac gctctattac gactggaaca acgaagaaga taaggcactg
    18781 gcaacacaat atggttttat ttgtgatacc cggcgttgta catggatgct ggacggtttg
    18841 aaaggctcaa tccaccagaa aaataaaaaa gcggattact caaacgtgat ggttttccat
    18901 caacccttta cggtgggatt ttatgagtac aaagcaacag atggcgtccc gcgcggcctc
    18961 gttaatgcgc tgctgccagt cactcttacg ctggatatag taacgtcacc actccagttc
    19021 ctgattttat gcacgaccag aaattgttaa tcgctgcggg caaacgcatt catgccgttt
    19081 gcccattcac taattaattc agcacatact tctcaatagc aaatgccacg ccatcttcaa
    19141 ggttggattt ggtgacaaag ttcgccactt ctttcaccga aggaatagcg ttatccatcg
    19201 ccacaccgac gcctgcatat tcaatcattg cgatgtcgtt ttcctgatcg ccaatcgcca
    19261 tgatttcttc cggtttaata cctaacacgt cggccagtga tttcacaccc gtacctttgt
    19321 taacgcgttt atcgaggatt tcgaggaagt acggcgcact tttcagcacg gtgtattttt
    19381 ctttcacttc ctgcggaata cgcgcgatgg cctggtcgag gatggcaggt tcatcaatca
    19441 tcatcacttt caggaactgg gtattggggt ccattttctc cgcttcgcag aacaccagcg
    19501 gaatggtggc aacgaaagat tcatgcaccg tgtagtagct gatatcacga ttggcggtgt
    19561 acagcgtggt gcggtccagg gcgtggaaat gagaaccgac ttcgcgagag agtttttcca
    19621 ggaaacggta gtcgtcatag ctgagagtgg tttgcgccac ggtgctacca tcagcggcct
    19681 tctgtaccag cgcgccgtta taagtaatgc agtagtcgcc cggctgttcc atatgcagct
    19741 ctttcaggta attgtgcaca cctgcataag ggcgacccgt cgttagcacg acattcacgc
    19801 cacgggcgcg agctgcggca atcgcatttt taacggcggg tgaaatggtg tgatcgggca
    19861 gcagaagggt gccatccata tcgatagcaa tgagtttgat agccatgagt tccccaggta
    19921 gattggttcc tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa cacccgaggg
    19981 aaaaggggga tgcaacgcgc gttgcgtgct ccccttttgc ttagcggaag agtttccctt
    20041 tcagcagttc catgcctgcg gaaagcagat cgttattggc ttgtggtgac acttcacctt
    20101 gcggtgagag tgcatcaata atcttcggca attgttctgc cagtaaactg gaagctgtac
    20161 tggtatccac gcccagtttt tgcccgagat cggacaccgc atttgtgccg agcgccgatt
    20221 ccagttgctc gccgctaact ggttggttgc cctgttgatt actcagccag gttgagagaa
    20281 tggcacctaa accgccactt tgcagttttt ccagcagcac ctgaatgccg ccctgctcct
    20341 caacccaact taaaatcgcc tgatatttcc ccgcatcgcc tttcagaaag gcgccgacaa
    20401 cgtcatcaaa aaggcccatg ataatcacct gtaaagcgtt acgtgttgac tcaaaaagta
    20461 tagttctgtg ggatgataat tgcggattgc aggaataaaa agggcggaga tgatctccgc
    20521 ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaaaca
    20581 tgcaaattca atatattgca ggagtctcgt aggcctgata agcgtagcgc atcaggcacg
    20641 ctcgcattgt taacgccatt aaatatcaat attcgccgct ttcagagcgt tctcttcaat
    20701 aaacgcacgg cgcggttcaa cggcgtcgcc cataagcgta gtgaacaact ggtcggcagc
    20761 aatcgcatct ttaacggtaa cgcgcagcat acgacggctt tccgggtcca tggtggtttc
    20821 ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg agaggccgcg
    20881 acgggactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg gctgacgacg
    20941 ttcgccacgt tcgataaatg catcttcttc cagcaagcca cgcagtttct cacccagcgt
    21001 gcagatacga cgatattcgc cgccagtgat aaattcgtga tccagcggat agtcagtatc
    21061 cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg ttttgttcgg cattggtgtg
    21121 gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc tgaccagcgc
    21181 gttcacccag cgggtaacgg tctgctcatc agagaggtcg gcttccgtca gcgtcggctg
    21241 atagataagc tctttcagca ttgctttcgg ataacgacgc tccatgcggt tgatcatttt
    21301 ctgcgtcgcg ttgtactcag acaccagttt ctctaacgct tcgccagcca atgccggtgc
    21361 actggcgttg gtgtgcagcg ttgcgccatc cagcgcgatg gagatctggt actgatccat
    21421 cgcttcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt acagcggcgg
    21481 ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat agaagaaggt
    21541 caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga tgatgatgct
    21601 gtgataacgc agtttgtccg ggttgtactc gtcacgaccg ataccacaac caagcgcggt
    21661 gataagcgtc gccacttcct gggaagagag catcttatcg aagcgcgctt tctcgacgtt
    21721 gaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc cctgcttcgc
    21781 agagccgccc gcggagtccc cttccaccag gtacagttca gagagcgccg gatcgcgttc
    21841 ctggcagtct gccagtttgc ccggcaggcc cgctaagtcg agcgcacctt tacggcgggt
    21901 catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa ttttgccaac
    21961 cacgattttc gcgtcggttg ggttttccag caggtattct gccagcagtt cgttcatctg
    22021 ctgctcaacc gccgatttca cctcagaaga aaccagtttg tctttggtct gggaggagaa
    22081 tttcgggtcc ggcactttca cggaaacgac cgcaatcagg ccttcacgcg catcgtcacc
    22141 ggtggcgctg actttggctt ttttgctgta gccttctttg tccatgtagg cgttcagggt
    22201 acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct gcggaatgtt
    22261 gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcaacg ccacttcgac
    22321 gccaataccg tctttctcgg tggagaagta gaagatattc gggtggatcg gcgttttgtt
    22381 cttgttcaga tattcaacga acgccttgat gccgccttca tagtggaagt ggtcttcttt
    22441 gccgtcgcgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg acaactcacg
    22501 cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg tttcgaggct
    22561 tggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg ccagcggggc
    22621 ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct ggataaccag
    22681 ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca gaccgccgga
    22741 cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca tgatcacttc
    22801 cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc cgtcatcctg
    22861 tacagagaca gagttatcgg cgtgaatggt gacgataatt tctttacagt gacccgcgag
    22921 cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac cggtgccgtc
    22981 atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc ctttcaggac
    23041 tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat ttatacttgg
    23101 gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg tccgacatgt
    23161 ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc gcttttaagc
    23221 ggctggcaag cagcccgcga cgctcatcat caagctcaga ggcaaaatca tctatcaggt
    23281 agagacaccg ccgcccgctt tcacgggtga ggaactctcc ttgcgccaga cgtaaggcgc
    23341 acatcaacag cttaagctgc ccacgcgata aggtatcttc caccggcgca ccgtcggcgc
    23401 gaatgcgtaa gtccgcttta tgcgggccgt gcgcggtgta ggttagctgg cgatcgcgtt
    23461 caaaattacg ttccagcacc tcagcgtact ctgtctcttt ctcccagccg cgctggaaag
    23521 agaaagtcag agaaaactca gggagaaatt gcttacaggt atcggccata tcagccgcga
    23581 taccggcgct atactccgcg cgccaggtgc ttatttgctc cgccagcgga atcagttctt
    23641 tatcccacgg gcgtagctgt tcgtaacgtg tcacctggcg cagcgccgca ttgcgctgct
    23701 tgagcaatcg cttgagattg ctccaggcgg tgaaaaatcc gggttcgttg tgaaagcatc
    23761 cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta aacccttctg
    23821 gcgttatcaa ctgcattggc atcaggtgcg ccagttccgc gaccttatgc ccgtctgtac
    23881 cgtcgatgcg gactttgctg tcgccctgtt tgtctttggt taagccaatc gccgtctcgc
    23941 gctcttcgcc ctgtaatcgc ccgtggagaa caaacgcctc ctgctcgtgg cgaatgacgc
    24001 gaccaatctg caaactgcga aacgcccgac catggccgag cgtatagatg gcttccagca
    24061 cgctggtttt gccactaccg ttggcaccta ccagaaagtt aaagccggga gataaagcga
    24121 gatccgcggt ttcgatattg cggaaatcgc ggatcaacaa gcgggtgagg gacattacag
    24181 tctcattggc atgacaacat aggcagccga ctgtgatgcg gcatcttcaa tctgcacgct
    24241 ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat ttcagtgcgt tcagaacatc
    24301 cagcacatag ctgacgttga agccgatttc catctccgca ccgctatagg taacgtcgag
    24361 gatctcttcc gcttcttcct gttctgggtt gttggcggtg attttcagct ggttttcgct
    24421 gacatacaga cgcacgccgc ggaatttctc gttagagaga attgccgcac gggcaaacgc
    24481 ctgcttgagc agatcacagc cagcttccag atgcttgtcc gggttcttcg gcagaacgcg
    24541 acggtaatcc gggaagcgac catccaccag tttcgaggtg aagataaagt cgccaacgtg
    24601 ggcgcgaatg ttgttgctgc caatctgcac gcgcagcgga ttgtcgccgc cgtcgagcat
    24661 acgcatcagt tcaatcacgc ctttacgcgg cacgatcacc gaatggcttg gcaaagattg
    24721 accaattggc attgaacaga ctgccagacg gtggccgtcg gtcgccacgg tgcgcagttc
    24781 ttcaccttcg gtttcaaaca gcataccgtt taagtaatag cgaacgtcct gatgcgccat
    24841 agaaaactgg gtcgcttcaa tcagacgctt catcgtcgcc tgcggcaggg taaattcgac
    24901 ttcgctctgc cagtcatcga ggttcgggaa atctgccgct ggcagggtag acagcgaaaa
    24961 acggctacgc ccggagcgca ccagcatccg ctcaccttcc agctgcacgg caatttccgc
    25021 gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg tcgtcgcccc
    25081 tggttcgtgc ggctgaacca gcgcaacgcg tgccaccatt tccatctcga gatcagtacc
    25141 ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa tcggtagcgt
    25201 aggacgacca cctaacggac cgctcacctg ttgtagcggt tttaataaat gctcacgttc
    25261 tacggtaaat ttcataggtt tacgatgaca atgttctgat taaattagaa aaatcttctt
    25321 tgatatcgtg gctctcttca cgcaactgct cgatcttccg gcaggcatga agcaccgtcg
    25381 tgtggtcacg accaccaaag gcatcgccaa tctccggaag actgtggtta gtcagctctt
    25441 tcgccagcgc catcgccatt tggcgcggac gggccaccga gcgggatcga cgcttggaaa
    25501 ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata ttgtcgatgg
    25561 tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc ctcacgcacg aagtcgatgg
    25621 tgatcgcccg tccggtaaag ttggcattgg caatgacgcg gttcagcgcg ccttccaact
    25681 cacgtacgtt agatcgtaga cgcttggcga taaaaaacgc cacttcgccc ggcaaacgaa
    25741 tgtcgttttc gtcggccttt ttcatcagga tcgccacgcg ggtttccagc tctggcggtt
    25801 cgatcgccac agtcagtccc cagccgaagc gggatttcaa acgatcctca acgccgttga
    25861 tctctttcgg atagcgatcg gaggtgagaa tgatctgttg attaccttcc agcagggcgt
    25921 tgaaggtgtg gaaaaactct tcctgagatc gttctttatt agcaaaaaac tgaatatcgt
    25981 cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc gcgttgtttt
    26041 gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc actttggcat
    26101 tcggcttgcg cgccataatg ccgttaccca ccgcatgcag caggtgagtt ttacccaaac
    26161 ccgtgccgcc ataaaggaac aacgggttat aagcaccgcc agggttatct gccacctggc
    26221 gagccgccgc gcgcgccagt tggttagatt taccttcaac gaagttatca aacgtgtgtt
    26281 tgacgtttac gttagaacga taggtcggtt ctgccggagc cgggacgtta tcccaacccg
    26341 agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca ggggccgcga
    26401 cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg acttcaaaac
    26461 gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata ttattaaggt
    26521 acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggcc agcgtgttat
    26581 cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct ggtaactcat
    26641 cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac tccactcgaa
    26701 caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg tcgaaaagac
    26761 cctgcatgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc aagatcctgc
    26821 aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct gtttctcaca
    26881 gattttcccg atttatccac aggactttcc agaactcgct aagtgtaaac gatcctgccg
    26941 cgggccgggc acgatttacg ccgcatattg gaaaatttaa tgaccataga caaaaattgg
    27001 cttaatcgat ctaataaaga tccaggacga tccttgcgct ttacccatca gcccgtataa
    27061 tcctccaccc ggcgcgccat gctggttttc actggtgtga ggccgtacat tttccctgcg
    27121 aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggag tgtacaatta
    27181 ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca tttttcggcg
    27241 gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca tgaaacgcac
    27301 ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc gtatggctac
    27361 taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc gtctgaccgt
    27421 ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc atttcccagg gagttacgct
    27481 tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct ggcacgccgc
    27541 aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt cttacagtcg
    27601 ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgtctg acgcgtgaaa
    27661 gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg gcgaaaaaag
    27721 gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta tggcgccgcc
    27781 actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac gcctgattag
    27841 tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg gaattgaggc
    27901 attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg tattaaaatg
    27961 ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg ataccagaga
    28021 acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt cgtgtctttc
    28081 atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca acagaccacg
    28141 cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc cagtggccag
    28201 gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac ccgtggtggt
    28261 gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac ccagccgttc
    28321 cagctgctgg aaacttcacc gcagtttatt tatcaggcac agagcggtct gaccggtcgt
    28381 gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga aaaggacgct
    28441 tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac cgacgcggca
    28501 ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt caacgtcaac
    28561 tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct cctcgtttgg tcagttgaag
    28621 caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc actgcacacc
    28681 ttccgtggcg cggcgtactc cacgcctgac gagaagtacg agaaatacaa gttcgatacc
    28741 attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc gatgctgcaa
    28801 cagtatttcg cgacggcatg gatcccgcat aacgacggta ccaacaactt ctataccgct
    28861 aatctgggta acggcatcgc cgctatcggc tataaatctc agccggtact ggttcagcct
    28921 ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca ggacaaaatg
    28981 gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg gttcatttct
    29041 cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg gggcttctcc
    29101 attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa agcgcagtac
    29161 acctccatgg cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg tgagcgtctg
    29221 ggcgatgaca aacagcgtat cagccaggaa atgatggcgc tgtacaaagc tgagaaggtt
    29281 aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct ggcgttgtac
    29341 tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg gatccacgac
    29401 ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac gatgttcttc
    29461 attcagaaga tgtcgccgac cactgtgacc gacccgatgc agcagaagat catgaccttt
    29521 atgccggtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt gctgtactat
    29581 atcgtcagca acctggtaac cattatccag cagcagctga tttaccgtgg tctggaaaaa
    29641 cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc gctaaaataa
    29701 gggcggtcag ttgaccgcct tttttctttt cgtagggcgg ataagcaccg cacatccgcc
    29761 acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca ggccacgcct
    29821 ccgggacgtg gcggcgttgg catcctgcgc atctccggct tcaaagcccg tgaagttgcc
    29881 gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc gtttaaagac
    29941 gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc gaactcgttc
    30001 accggcgaag atgtgctgga actgcaaggt catggcggtc cggtgatcct cgacctgctg
    30061 ttaaaacgca ttctgaccat tcccggcctg cggattgctc gcccgggtga gttttccgaa
    30121 cgcgcgtttc ttaacgataa actcgactta gcccaggccg aggcgattgc ggatcttatc
    30181 gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg cgcattctcc
    30241 gcacgggtta atcatctggt agaagccctc acccacttgc gcatttacgt tgaagcggca
    30301 attgatttcc ccgatgaaga gatcgatttc ctctccgacg gcaaaattga agcccagctt
    30361 aatgacgtga ttgccgatct cgatgcagtg cgtgctgaag cacgtcaggg tagtttgttg
    30421 cgcgaaggga tgaaagtggt gattgccgga cgtcctaacg ccggtaaatc gagcctgtta
    30481 aacgcgctgg ctggccgtga agcagcaatc gtaaccgata tcgccggaac tacgcgtgac
    30541 gtgttgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga caccgccggg
    30601 ctacgtgaag ccagcgatga agtggaacgt attggtatcg agcgcgcgtg gcaagaaatt
    30661 gaacaagccg accgcgtgct gtttatggtc gatggcacca cgacagacgc cgttgatcca
    30721 gcagagatct ggccggagtt tatcgcccgt ctgccagcga aactgccgat caccgtggtg
    30781 cgcaataaag ccgatatcac cggagaaacg ttggggatga gcgaagtgaa cggtcacgcg
    30841 ttaattcgtc tctctgcaag gactggtgaa ggtgtggacg tgctgcgtaa ccatctcaaa
    30901 cagagcatgg gctttgacac caacatggaa ggcggcttcc tggcgcgtcg tcgtcaccta
    30961 caggcactgg aacaggcggc agagcacctg caacagggta aagcgcaact gttaggagcc
    31021 tgggcaggcg agctgctggc ggaagaactg cgtctggcgc agcagaactt aagcgagatc
    31081 accggggaat ttacttctga cgacctgctg gggcgaattt tctccagctt ctgtattggt
    31141 aagtaaccgc gcttgcgaag ccgcattctg actgtcagat gcggcttcgc ttcat
//
LOCUS       DAZKJR010000050        30495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKJR010000050 DAZKJR010000000
VERSION     DAZKJR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.050"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..570
                     /locus_tag="ACHOWS_002274"
     CDS             <1..570
                     /locus_tag="ACHOWS_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFQ0047839.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIH
                     PIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            800..1183
                     /gene="secE"
                     /locus_tag="ACHOWS_002275"
     CDS             800..1183
                     /gene="secE"
                     /locus_tag="ACHOWS_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0047840.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1185..1730
                     /gene="nusG"
                     /locus_tag="ACHOWS_002276"
     CDS             1185..1730
                     /gene="nusG"
                     /locus_tag="ACHOWS_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0047841.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1889..2317
                     /gene="rplK"
                     /locus_tag="ACHOWS_002277"
     CDS             1889..2317
                     /gene="rplK"
                     /locus_tag="ACHOWS_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0047842.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2321..3025
                     /gene="rplA"
                     /locus_tag="ACHOWS_002278"
     CDS             2321..3025
                     /gene="rplA"
                     /locus_tag="ACHOWS_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0047843.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3438..3935
                     /gene="rplJ"
                     /locus_tag="ACHOWS_002279"
     CDS             3438..3935
                     /gene="rplJ"
                     /locus_tag="ACHOWS_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0047844.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4002..4367
                     /gene="rplL"
                     /locus_tag="ACHOWS_002280"
     CDS             4002..4367
                     /gene="rplL"
                     /locus_tag="ACHOWS_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0047845.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4687..8715
                     /gene="rpoB"
                     /locus_tag="ACHOWS_002281"
     CDS             4687..8715
                     /gene="rpoB"
                     /locus_tag="ACHOWS_002281"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0047846.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8792..13015
                     /gene="rpoC"
                     /locus_tag="ACHOWS_002282"
     CDS             8792..13015
                     /gene="rpoC"
                     /locus_tag="ACHOWS_002282"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0047847.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13255..14388)
                     /gene="thiH"
                     /locus_tag="ACHOWS_002283"
     CDS             complement(13255..14388)
                     /gene="thiH"
                     /locus_tag="ACHOWS_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFQ0047848.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQVKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSISFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14385..15155)
                     /gene="thiG"
                     /locus_tag="ACHOWS_002284"
     CDS             complement(14385..15155)
                     /gene="thiG"
                     /locus_tag="ACHOWS_002284"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFQ0047849.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIITQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15157..15357)
                     /gene="thiS"
                     /locus_tag="ACHOWS_002285"
     CDS             complement(15157..15357)
                     /gene="thiS"
                     /locus_tag="ACHOWS_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFQ0047850.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQSGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15341..16096)
                     /gene="thiF"
                     /locus_tag="ACHOWS_002286"
     CDS             complement(15341..16096)
                     /gene="thiF"
                     /locus_tag="ACHOWS_002286"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFQ0047851.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRNLALRRASGCPVCGGSNADPV"
     gene            complement(16089..16724)
                     /gene="thiE"
                     /locus_tag="ACHOWS_002287"
     CDS             complement(16089..16724)
                     /gene="thiE"
                     /locus_tag="ACHOWS_002287"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFQ0047852.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16724..18619)
                     /gene="thiC"
                     /locus_tag="ACHOWS_002288"
     CDS             complement(16724..18619)
                     /gene="thiC"
                     /locus_tag="ACHOWS_002288"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFQ0047853.1"
                     /translation="MSVTKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGREHIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18700..18800)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18852..19328)
                     /gene="rsd"
                     /locus_tag="ACHOWS_002289"
     CDS             complement(18852..19328)
                     /gene="rsd"
                     /locus_tag="ACHOWS_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFQ0047854.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19423..20196
                     /gene="nudC"
                     /locus_tag="ACHOWS_002290"
     CDS             19423..20196
                     /gene="nudC"
                     /locus_tag="ACHOWS_002290"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFQ0047855.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20236..21300
                     /gene="hemE"
                     /locus_tag="ACHOWS_002291"
     CDS             20236..21300
                     /gene="hemE"
                     /locus_tag="ACHOWS_002291"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFQ0047856.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSAPYHL"
     gene            21310..21981
                     /gene="nfi"
                     /locus_tag="ACHOWS_002292"
     CDS             21310..21981
                     /gene="nfi"
                     /locus_tag="ACHOWS_002292"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFQ0047857.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22024..22614
                     /gene="yjaG"
                     /locus_tag="ACHOWS_002293"
     CDS             22024..22614
                     /gene="yjaG"
                     /locus_tag="ACHOWS_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFQ0047858.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22801..23073
                     /gene="hupA"
                     /locus_tag="ACHOWS_002294"
     CDS             22801..23073
                     /gene="hupA"
                     /locus_tag="ACHOWS_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFQ0047859.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23086..23781
                     /gene="yjaH"
                     /locus_tag="ACHOWS_002295"
     CDS             23086..23781
                     /gene="yjaH"
                     /locus_tag="ACHOWS_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFQ0047860.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23783..24202)
                     /gene="zraP"
                     /locus_tag="ACHOWS_002296"
     CDS             complement(23783..24202)
                     /gene="zraP"
                     /locus_tag="ACHOWS_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFQ0047861.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMDHW"
     gene            24440..25816
                     /gene="zraS"
                     /locus_tag="ACHOWS_002297"
     CDS             24440..25816
                     /gene="zraS"
                     /locus_tag="ACHOWS_002297"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFQ0047862.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGTGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQVGKGATFTLWLPVT
                     ITRKDPQG"
     gene            25813..27138
                     /gene="zraR"
                     /locus_tag="ACHOWS_002298"
     CDS             25813..27138
                     /gene="zraR"
                     /locus_tag="ACHOWS_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFQ0047863.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENTVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27135..28424)
                     /gene="purD"
                     /locus_tag="ACHOWS_002299"
     CDS             complement(27135..28424)
                     /gene="purD"
                     /locus_tag="ACHOWS_002299"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0047864.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28436..30025)
                     /gene="purH"
                     /locus_tag="ACHOWS_002300"
     CDS             complement(28436..30025)
                     /gene="purH"
                     /locus_tag="ACHOWS_002300"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0047865.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7141 a         7904 c         8415 g         7035 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggtc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
      421 gaaggtgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      481 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      541 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      601 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      661 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      721 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaat
      781 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      841 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtagcgattg tcggcaacta
      901 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      961 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagca accgttgctt ttgcccgtga
     1021 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
     1081 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
     1141 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
     1201 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
     1261 tgcgtgagca tatcaaatta cacaacatgg aagagttgtt tggtgaagtc atggtaccaa
     1321 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1381 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacctg gtgcgcagcg
     1441 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1501 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1561 cgttgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1621 gcgttgttga agaagttgat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1681 gtcgtgcgac ccctgtagag ctggacttca gccaggttga aaaagcttaa cccagcgatc
     1741 aaaaaagcgg cgatttaatc gttgcacaag gcgcgagatt ggaatacaat ttcgcgcctt
     1801 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1861 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1921 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1981 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     2041 aggtctgccg attccggtag taatcactgt ttacgctgac cgttctttca ctttcgttac
     2101 caagactccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     2161 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     2221 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     2281 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     2341 catgcgtgtt atccgagaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2401 cgctctgctg aaagaactgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2461 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2521 cggtactggc cgttccgttc gcgtagccgt atttacccag ggtgcaaacg ctgaagctgc
     2581 taaagctgca ggtgctgaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2641 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2701 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacgcc
     2761 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2821 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2881 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2941 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     3001 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     3061 tacccccacg tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     3121 cggtaactga gtgagtcgcc aggagcatag ttaactatgt gactggtgcg aacgaaggaa
     3181 gccaacgccg ccacaagctg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     3241 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     3301 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3361 ttaagacgct ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3421 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3481 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3541 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3601 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3661 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3721 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3781 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3841 caattgcacg cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc
     3901 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3961 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     4021 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     4081 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     4141 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     4201 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     4261 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     4321 tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4381 gttgcagcct gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg
     4441 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4501 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4561 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4621 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4681 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4741 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4801 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4861 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4921 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4981 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     5041 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     5101 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     5161 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct
     5221 taccgtggtt cctggcttga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc
     5281 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag
     5341 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5401 atggaactgg tgccggaacg cctgcgtggt gaaaccgcat cttttgacat cgaagctaac
     5461 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5521 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5581 aaagactata ttgatgagtc taccggcgag ctgatctgtg ctgcgaacat ggagctgagc
     5641 ctggatctgc tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc
     5701 aacgatctgg atcacggccc atatatctct gaaaccttac gtgtcgaccc aactaacgac
     5761 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5821 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacttgtct
     5881 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5941 attctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     6001 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa
     6061 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     6121 ctgtctctgg gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccgatt
     6181 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     6241 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc aggcggtctg
     6301 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta
     6361 tgtccaatag aaacccctga aggtccgaac atcggtctga tcaactctct gtccgtgtac
     6421 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt
     6481 gtaactgacg aaattcacta cctatctgct atcgaagaag gcaactacgt tatcgcccag
     6541 gcgaactcca acctggatga agaaggccac ttcgtagaag acctggtaac ctgccgtagc
     6601 aaaggcgaat ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag
     6661 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6721 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgacaagccg
     6781 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct
     6841 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6901 gacgagatgt atccgggtga agcaggtatc gatatctaca acctgaccaa atacacccgt
     6961 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa
     7021 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     7081 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     7141 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     7201 gtgtcccgtg acaccaagct ggggccagaa gagatcaccg ctgacatccc gaacgtgggt
     7261 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc
     7321 ggtggcgaca ttctggttgg taaggttacg ccgaaaggtg aaactcagct gaccccagaa
     7381 gaaaaactgc tgcgtgcgat cttcggtgag aaagcgtctg acgttaaaga ctcttctctg
     7441 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7501 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7561 gacctgtctg aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg
     7621 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7681 gagctgggcc tgaccgacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7741 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7801 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc
     7861 cgtatccagc ctggtgacaa gatggcaggt cgtcatggta acaagggtgt aatttctaag
     7921 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7981 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     8041 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     8101 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     8161 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     8221 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     8281 ctgaaacttg gcgacctgcc gacttctggt cagatccgcc tgtacgacgg ccgcactggt
     8341 gaacagttcg agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8401 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8461 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8521 gaagcatacg gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg
     8581 aacggtcgta ctaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8641 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8701 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8761 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8821 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8881 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8941 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     9001 cctgtgcggt aagtacaagc gcctgaaaca ccgtggtgtc atctgtgaga agtgcggcgt
     9061 tgaagtgacc cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc
     9121 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggcctgc tgctcgatat
     9181 gccgctgcgc gatatcgaac gcgtactgta cttcgaatcc tatgtggtta ttgaaggcgg
     9241 tatgaccaac ctggaacgcc agcagatcct gactgaagag cagtatctgg acgcgctgga
     9301 agagttcggt gacgaattcg acgcgaaaat gggtgcggaa gcaatccagg ccctgctgaa
     9361 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9421 cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9481 tggtaacaaa ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg
     9541 tccgctggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9601 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9661 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9721 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat
     9781 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9841 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9901 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9961 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
    10021 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
    10081 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
    10141 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg cagttcacgt
    10201 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
    10261 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct
    10321 gtactacatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10381 gaaagaagca gaacgtctgt atcgctctgg tctggcctct ctgcatgcgc gcgttaaagt
    10441 gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa
    10501 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc
    10561 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10621 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggctttgc
    10681 atatgcagcg cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa
    10741 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10801 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10861 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtta ttaaccgtga
    10921 cggtcaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10981 gcgtggttct gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc
    11041 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    11101 ccagtacttc atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac
    11161 ggcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    11221 cgaagacgat tgtggtactc atgaaggtat catgatgact ccggttatcg aaggtggtga
    11281 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    11341 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11401 cctgctggaa gagaactctg tcgacgcggt taaagtacgt tctgttgtat cttgtgacac
    11461 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11521 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11581 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11641 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11701 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11761 taccaaagaa agctacaaag taccttacgg tgcggtactg gcaaaaggcg atggcgaaca
    11821 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11881 agtaagcggt tttgtacgct ttactgacat gatcgacggt cagaccatta cgcgtcagac
    11941 cgacgaattg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    12001 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg atgtactgat
    12061 cccgggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    12121 agatggcgta cagatcagct ctggtgacac cctggcgcgt attccgcagg aatctggcgg
    12181 taccaaggac atcaccggtg gtctgccgcg cgttgcggat ctgttcgaag cacgtcgtcc
    12241 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    12301 aggtaaacgt cgtctggtta tcactccggt agacggtagc gatccgtacg aagagatgat
    12361 tccaaaatgg cgtcagctca atgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12421 ttccgatggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgttac
    12481 tcgttacatc gttaacgaag tacaagacgt ataccgtctg cagggcgtta agattaacga
    12541 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12601 cagctccgac ttcctggaag gtgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12661 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12721 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12781 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12841 cgttatcgtg ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat
    12901 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12961 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    13021 aatccgcaaa taacgtaaaa actcgcttcg gcgggttttt ttatgggtgt tattcagagg
    13081 gcaattcttc acggaatttt taagaaaggc agggcgaaac gaggaagaag cttttcgccc
    13141 tggacaacat tcctgctgac gggactacca taaaaaaaca caacgctttc aacaaccttg
    13201 cgtttttcat cttccgtagc ggctcggaat aaatttacgt tgttacattc tgtctcatgg
    13261 cctttgcgag gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg
    13321 agcagttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc
    13381 cagctcgggg tgattgtcgg catagccacc tggctgcgtt ttcgaaaaag cgctgacgtt
    13441 attaattgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga
    13501 cagttcaatc tccggtgcaa gcagccggaa ggcgcagatg gtttgcacta actggcgttc
    13561 atccataatc gacgcaggct caatgccgcc agtacatgga cgcaggcgtg ggaaggagat
    13621 agagtagcgg ctttgccagt aatgctgttg cagccatagc aaatgttctg caaccatata
    13681 gcagtcaacg cgccagctgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat
    13741 ccccgcacgg cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttacc
    13801 tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa ccatcacgcc
    13861 atccagacct agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat
    13921 ctgtagtgaa gagaactgtt cacgcagggc agggagatga cgacgaaagt aatccatccc
    13981 cactttcacc tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat
    14041 agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattgct
    14101 catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac
    14161 gtagaagctg acgacgttgc caaatcgctg acgggtcaga cgctgcgccc gttgggccag
    14221 tggttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt cgcgggtgag
    14281 ttgcgaggca tttagcgccc gctctacgtc agcagccgtt ttgctgttga tacgcaggcg
    14341 gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc tgatgcctcc
    14401 agaaatccgg tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc cggtccggac
    14461 tgacgtgcca gcaggcctgc ttctactgcc agacgaaatg ccttcgccat gttgacggga
    14521 tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc
    14581 tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggaac cgtggcttgc
    14641 tgggtaataa tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg
    14701 ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg
    14761 gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg
    14821 gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa ttttaaccag
    14881 tttgtgccta acgcttcacg agccagatgg gcggcgaaaa tggcttcttc cgctgttttc
    14941 gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc gaggatagcg
    15001 tcgttgtgct ggcgcaagtt gacgcgtttc atcgccagtg tcaccagttg gctgccgcaa
    15061 gcgcggatcg cctccatcat cagttgtgaa gaggcgaatt tccctgtgcc ggtaaacaga
    15121 tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa
    15181 aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac gcgggacgat
    15241 ttgctgatta atcgccagag ccgcgcccga ttgtcgttgg tcgagttgct ccagtagttc
    15301 gtgaaccgtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc
    15361 ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggttgcgc cactggctcg
    15421 atttaccgtc gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa
    15481 tggcttccag tgcctgtaaa gtgcccataa ccccgaccac cgggccaacc acgcccgccg
    15541 tgcggcagtt gcgttcaggc tcctggttat ctggccacag gcagcggtaa cacccctgct
    15601 cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa
    15661 gcggcgtgtt gagtgccacg caggcggcat taatctcctg acgagtcgcc atattgtcgg
    15721 tacagtcgag caccacatcg gcccgtgcta ccgcatcttt taacgcctca cccgttaacc
    15781 gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg tgtcagtcgc tgatggctga
    15841 cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt tgcagattgc
    15901 ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg cccgccaggt
    15961 atagcgcagc aggtgtaccc agcccgccaa gaccgataat cagcacctgg ctgtcgagca
    16021 gtttttgctg cccgtcaaga gcgatatcgt cgagcaggat ttggcggcta taacgcataa
    16081 agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc
    16141 cagtctgcgg cttgagtaat ggcgcttacg acggcgatgc tgccgacacc cgttgctatc
    16201 accgcaggcg cgcgcgccag actgataccg ccaatcgcca cggtgggata atccgccagg
    16261 cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt
    16321 tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg
    16381 atctccatat cgtcatgggt cgaaacgccc agtcgcagac ctgccgcgcg gatggcgctg
    16441 agatcggtgg cttgcaaatc ttcctgcccc agatggacgc catatgcctg atgcttgatc
    16501 gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt
    16561 gccgccacga catcggcttc cacctcttca tcgcgtcgat ctttgatgcg tagctggaga
    16621 gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac
    16681 agtcctaaac gaaagggtac aggaggaaaa tctggctgat acatcacgct tcctccttac
    16741 gcaggtagat ttctccgcct ctggcgcgga agttctccga catatcagcc attcccactt
    16801 caatggtttg cgcggcggcg taatcgcgca cttcctggct gattttcatc gagcagaatt
    16861 tcggcccaca catggagcaa aaatgggcga ctttgccgga ttcttgcggc agggtttcat
    16921 cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa
    16981 attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgccctttcg
    17041 ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta acatcttctt
    17101 tattaggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc
    17161 agccaatcat cgccgcacca atccccgacg tgaagtggtc ataacccggc gcgatatcgg
    17221 tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca
    17281 tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc acctgtacgt
    17341 catattccca ggcgattttg gtcagttcac ccagcgtatg cagctcggca aattgcgctt
    17401 catcgttggc gtcctgaata gaaccagggc gcagaccgtc tcccagcgac agcgaaacgt
    17461 cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat
    17521 gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc
    17581 gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca
    17641 caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg
    17701 cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccgggc
    17761 tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca
    17821 ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag
    17881 aggtgaccgc cgagttgccg atattggcgt taacttttac caggaaattg cgaccaataa
    17941 tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcgcgt ccggcagcaa
    18001 cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca aagctcattc
    18061 ccggatgctg gtggcgtaaa acttcgctac gtatgtgctc gcggcccata ttctcgcgga
    18121 tggcgatgaa ttccatttcc ggtgtgatga tgccctggcg ggcgtagtgc agttgggtga
    18181 cgcggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc agctcgtcga
    18241 ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta agttcttcgg
    18301 tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagtccttgc tgcacgttaa
    18361 tggcgatctg tggatcgcca tacggaccgg aggtgtcgta gaccggaatc gcttcgtttt
    18421 cttcgtactg cggttgttct ttgctgccgc caattagcgt cgggctaagc tggatctcac
    18481 gcatcggcac gcgcacgccg gggtgtgtgc cagtgatgta aatgcgtttt gagttgggaa
    18541 aggcggtgcc ttccagggtg tcgataaaat gttgggcctg ggcgcgttgt tcgcggcggg
    18601 tcagttttgt tacagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac
    18661 ggatgaagca agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc
    18721 ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta
    18781 aggcactccg acaagaaatt agccccacaa acggggcgtt gaatgtaaat tacgcgttaa
    18841 cagcgcagaa ctcaagcagg atgtttgacg cgggcggcgt caagtaccag cagaatcaac
    18901 ttatcttcca gcacaaagcg tgcttccagc gcctcgccga tgtcagataa aacctgttga
    18961 aactcaaggt aattatcatg atcgatggcg gtttccaggc tggaatcgta gtaatccata
    19021 atctgttggg tattggcttc gagttgcggc caaatcttcg cggcgcgcgc gagttgcccg
    19081 ttgccttcca gcttatgaag aatgcgctca taaatactga aatgtccggc agacaagtaa
    19141 tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat gtacgattct
    19201 ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt acgtacatgt
    19261 agccagcgat caaccagttt gttacttcct ctgacgcgtt ccgtcaggtt atcgagctgg
    19321 ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga aatgtaaaaa
    19381 ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg tataattgaa
    19441 aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt gccgaaggga
    19501 gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc actacaaatc
    19561 ggcgaatggc aaggggaacc tgtttggtta gtacaacagc agcggcgtca cgatatgggg
    19621 tcggtacgtc aggtcattga tctcgatgta gggctgtttc aactggccgg acgtggcgtg
    19681 caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca tgaaatgtat
    19741 ccaagcaaaa ccgaatgggc gatgctgtgc agccattgcc gtgagcgtta ctacccgcaa
    19801 atcgccccct gcattattgt tgccatccgt cgcgatgatt cgatcctcct cgcccagcat
    19861 acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt gggtgaaacc
    19921 ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt taaaaacttg
    19981 cgttacgtga cctcccaacc gtggccgttt cctcagtcgt taatgaccgc gtttatggcg
    20041 gaatatgaca gcggcgacat cgttatcgac ccgaaagaat tgctcgaggc gaactggtat
    20101 cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg tctgatagaa
    20161 gatacggtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg cctgacgcac
    20221 taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc gctgctgcgc
    20281 cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta tctaccggaa
    20341 tataaagcca cgcgcgccca ggcgggcgat tttatgtcgc tgtgcaaaaa tgccgagctg
    20401 gcgtgcgaag tgactttgca accgctgcgt cgctatccgc tggatgcggc gatcctcttt
    20461 tccgatatcc tcaccgtgcc agacgcgatg ggtttagggc tctattttga agccggagaa
    20521 ggtccgcgtt ttacctcacc agtcacctgc aaggccgacg tcgataaact gccaattccg
    20581 gacccggaag atgagctggg ttacgtgatg aacgcggtgc gtaccattcg tcgcgaactg
    20641 aaaggcgaag tgccgctgat tggtttttcc ggcagcccgt ggacgctggc gacctatatg
    20701 gtggaaggcg gcagcagcaa agcgttcacc gtgatcaaaa aaatgatgta tgccgatcca
    20761 caggcgctgc acgcgctact cgataaactg gcgaaaagcg tcactttgta tctgaatgcg
    20821 cagattaaag ccggtgctca ggctgtgatg attttcgaca cctggggcgg cgtgcttacc
    20881 gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga tggtttactg
    20941 cgtgaaaacg acggtcgccg cgtaccggtc acgctgttta ccaaaggcgg cggacagtgg
    21001 ctggaagcca tggcagaaac cggttgcgat gcgctgggcc tcgactggac aacggacatc
    21061 gccgatgcac gccgccgcgt gggcaataaa gtcgcgttgc agggcaatat ggatccgtcg
    21121 atgctgtacg cgccgcctgc ccgcattgaa gaagaagtag cgactatact tgcaggtttc
    21181 ggtcacggtg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga tgtgccgcca
    21241 gaacatgctg gcgtgtttgt ggaggcagtg catcgcctgt ctgcgcctta tcatctgtaa
    21301 ggagtggtta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc ttcttctgtg
    21361 atccgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc cgatgtcgga
    21421 tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata cccctcgctt
    21481 gagctggtgg agtataaagt ggcccgcatc gccaccacca tgccttacat tcctggtttt
    21541 ctctcctttc gtgaatatcc tgcgctgctg gcagcgtggg agatgctgtc acaaaagccg
    21601 gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg cgtcgccagc
    21661 cattttggtt tgctggtgga tgtgccgacc attggcgtag cgaaaaaacg gctctgcggt
    21721 aaattcgaac cgctctccag cgaaccgggc gcgctggccc cgctgatgga taaaggcgag
    21781 cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cattgtttat cgctaccggc
    21841 catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa aggctatcgt
    21901 ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc gttcgtgcgc
    21961 tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat ttgagaactc
    22021 aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg gcagcacgtc
    22081 acttttatgg cctgcttatg cgaacgtatg taccccaatt acgccgtatt ctgtcagcaa
    22141 accggcttcg gcgatgggca gatttaccgc cgcattctcg atctcatttg ggaaacgctg
    22201 accgtcaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga agaagcgatt
    22261 ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc ttgcgtggcg
    22321 ttaagtgaac tggtccattc gcgtttaagt ggcgaaacgc tcgaacacgc ggtggaagtg
    22381 agtaagactt ccatcacgac cgtggcgatg ctggaaatga ctcaggctgg tcgcgaaatg
    22441 agcgatgaag agctcaaaga aaacccagct gtagagcaag aatgggacat tcagtgggaa
    22501 atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa aggccttagg
    22561 gcagacctgc gtgaggcggg tgagagcaat attggtataa tttttcagca ataagaccag
    22621 aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc cccccgtctg
    22681 gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg agagtgctat
    22741 tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta aggataactt
    22801 atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc caaaacccag
    22861 gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa agaaggcgat
    22921 gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga gcgtactggc
    22981 cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc ggcatttgtt
    23041 tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa cagttttaac
    23101 gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct ggctggcgct
    23161 ctgtttctca ctgcttgtag tcacaactct tcacttcctc cctttaccgc cagtggattt
    23221 gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa tgtgcatctt
    23281 cttgccgtgt ttagcccgtg gcgcagtggc gatacgacga cgcgagagta tcgctggcag
    23341 ggcgataacc tcacgctcat caatatcaat gtttacagca aacccccggt gaatattcgc
    23401 gcgcgttttg acgatcgcgg cgatctgagc tttatgcaac gcgaatccga tggggaaaag
    23461 cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgatca gatccgccag
    23521 attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg gcatgcgatg
    23581 gagcagactg tgaccacctg cgaagggcaa accattaaac ctgatttaga ttcgcaggcg
    23641 atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt ggcatggctg
    23701 gaagcgcccg aaggttcgca attactgttg gtggcaaact ctgatttctg tcgctggcaa
    23761 cccaacgaga aaacgttctg atttaccagt ggtccatacc catatgaccg ccaccaccgc
    23821 agccgccgta acccattccg gtaccgcgcg gaatacccgc ttcagccatc gcaatatctc
    23881 gtttcacccg taactcatct aacgactgac gcaaattctc catctctttg gcgaccgcgt
    23941 taattttgct gctatccggt gggttcgcgg ctaacagggc attgtattca taacgctttg
    24001 ttaccagttg ctgttgcagt gcgctgcttt gagcgtaaaa gtcattatgg attttctgcc
    24061 acgctgtctg ttgttcgctg gtcaaaggcg cggcattttg ctgccacata ccgtgtccgc
    24121 cgtgagcaaa tgccgatgtc gatcccatcg ccattgctga aagcgccatc attaccaggg
    24181 caattttcgt gttccgtttc atggttaatc ctccagtggt tgtcttactt cgggtattgc
    24241 atcttccgtg ccaacgatga aacgctgata tgacgggtaa tctggcatga taaacgagta
    24301 aaaatgactc gcctgctgcg ggtagcgagt catttttact cattgaaact taagcctttg
    24361 tgttacagcg cagggtaagc gctgataaaa gatggcatga tttctgctgt cagaaaggga
    24421 tgagcaggca aagaagaaga tgcgttttat gcaacgttct aaagactcct tagctaaatg
    24481 gttaagcgcg atcctccccg tggtcattgt tgggctggtg gggctgtttg cggtgacagt
    24541 gattcgcgat tacgggcgcg agactgcagc cgccagacaa acgctgctgg aaaaaggcag
    24601 tgtacttatc cgtgctctcg aatccggctc gcgcgtcggc atggggatgc gcatgcatca
    24661 tgcgcagcag caggcattac tggaagaaat ggccgggcag cctggtgtac gttggtttgc
    24721 ggtcacggat gaacaaggaa caatcgtgat gcatagcaac tccggcatgg tgggaaaaca
    24781 gctttattcc ccgcaggaaa tgcagcagtt acatccggga gatgaagaag cgtggcggcg
    24841 gatcgatagc gcagacggcg agcctgttct ggaaatttat cgccagtttc aaccgatgtt
    24901 tgctgctgga atgcaccgga tgcgccatat gcagcagtat gccgcgacac cacaagcaat
    24961 ttttatcgct ttcgacgcca gtaacattgt gagtgccgaa gatcgtgagc agagaaacac
    25021 cctgattatc ctcttcgccc tggcgacggt cttgctggca agcgtgttgt cattcttctg
    25081 gtatcgccga tatctgcgct cgggccagct gttgcaggat gaaatgaagc gcaaagagaa
    25141 gctggtggca ctggggcatc ttgcggcagg cgttgcccac gaaatccgta acccactttc
    25201 ctcgattaaa gggctggcga aatactttgc cgaacgcgcg ccagcagggg gagaagcgca
    25261 tcaactggcg caggtgatgg cgaaagaagc cgaccgttta aaccgcgtgg taagcgagtt
    25321 gctggaactg gttaagccaa cgcatctggc tttgcaggcg gtggatctca acacgctgat
    25381 taaccactca ttacagctgg taagccagga tgcaaacagc cgggagatcc agttacgctt
    25441 taccgccaac gacacattac cggaaattca ggccgatccg gacaggctga ctcaggtcct
    25501 gttgaatctc tatctcaatg ctattcaggc gattggtcag catggcgtga ttagcgtgac
    25561 ggccagcgaa agcggcacgg gtgtgaaaat cagcgttacc gacagcggta agggaattgc
    25621 ggcggatcag cttgaagcca tcttcacccc gtatttcacc acgaaggccg aaggcaccgg
    25681 actggggctg gcggtcgtgc ataatattgt tgaacaacac ggtggtacaa ttcaggtcgc
    25741 aagccaggtg ggaaaaggcg caacgttcac cctctggctt ccggtcacta ttacgcgtaa
    25801 ggacccacaa ggatgacgca cgataatatc gatattctgg tggtggatga tgacattagc
    25861 cactgcacta ttttgcaggc tttgctgcgc ggctggggct ataacgtcgc gctggcgaac
    25921 agcgggcgac aggcgctgga gcaggtgcgg gaacaggttt ttgatcttgt gctttgcgat
    25981 gtgcgaatgg cggagatgga cggcattgcc acgctaaaag agatcaaagc gctaaatccg
    26041 gcgattccgg tactgattat gaccgcatac tccagcgtcg agacggcggt agaggcactg
    26101 aaaaccgggg cgctggatta tctcatcaag ccgctggatt tcgataacct gcaggcgacg
    26161 ctggaaaagg cgctcgcaca tacgcacagt gttgacgctg aaacgcctgc ggtgtctgcc
    26221 agccagttcg gtatggtagg taaaagcccg gcgatgcaac acctgctcag tgaaatcgcc
    26281 ctcgtcgcgc catcggaagc cacagtgctg atccacggcg attccggcac cggtaaagag
    26341 ctggtcgcaa gggcgattca cgccagtagc gcacggagtg aaaaaccact ggtaacgctc
    26401 aactgtgcgg cactcaacga atccttgctg gaatctgaat tgttcggtca cgaaaaaggg
    26461 gcgtttaccg gagccgataa acgccgggaa gggcgctttg ttgaggcgga cggcggcacg
    26521 ctgtttctcg atgaaattgg cgatatctcg ccgatgatgc aggttcgtct gctgcgtgcg
    26581 attcaggagc gcgaagttca gcgtgtcggc agcaaccaga ccatctcggt tgatgtccgg
    26641 ctgattgcgg cgactcatcg cgatcttgcc gcagaggtga atgccgggcg ttttcgccag
    26701 gatctctact atcgcctgaa cgtggtggcg attgaagttc cgtcgctgcg tcagcggcgg
    26761 gaagatattc ctctgctggc aaaccatttt cttcagcgct ttgccgagcg taatcgtaag
    26821 gcggtaaaag gttttacgcc ccaggcgatg gatctgctga ttcactacga ctggccggga
    26881 aatattcgtg aactggaaaa cacggtggaa cgggcggtgg tgctgctgac cggggaatat
    26941 atttccgaac gcgagctgcc gctggcgatt gcgagtacgc cgatcccgct ggtacaaagt
    27001 caggatattc agccgctggt ggaagtcgaa aaagaggtga ttctggcggc gctggagaaa
    27061 acgggcggca acaaaaccga agccgcccgc cagttaggga tcacgcgcaa aacgctattg
    27121 gcaaaactgt cgcgttagtt ctgctcgcgt tcgatagcgc gccagccgat atctttccgg
    27181 cagaagcagt cgtcccaatg gatatcggtc attaaggcat aagcgcgttt ctgcgcttct
    27241 gccacggtat gacccagcgc ggtaacgcac agtacgcgcc cgccgctggt cactacctgc
    27301 tcgtcatccg ctagtttcgt gcccgcgtgg aacactttgc cgtctgccac ttcttccagc
    27361 ggcaggccgt ggatcacatc gccggtgcgg taatcgcccg gatatccacc cgcagccatc
    27421 accacgccga gagaagcgcg ttcatcccac tcggacgttt tctcgtccag cttgccttca
    27481 caggccgcca ggcagagctc aaccagatcg gacttcatgc gcagcataat cggctgggtt
    27541 tctggatcgc caaagcggca gttaaattca ataaccttcg gattgccctg tttgtcgatc
    27601 atcaggcccg cgtagagaaa accagtgtag gtgttgcctt ctgccgccat gcctttcacg
    27661 gttggccaga tgattcgttc catcgtgcgc tgatgaactt catcggttac taccggcgcg
    27721 ggggagtaag cgcccatccc gcccgtgttt ggtccggtat ctttatcgcc tacgcgtttg
    27781 tgatcctggc tggtagccat cggcagcaca tgctcgccgt ccaccatcac gataaagctc
    27841 gcttcttcgc catcgaggaa ctcttcgata acgatgcgat gacccgcgtc gccaaacgcg
    27901 ttgcccgcca gcatatcgtg aacagctgct tcagcttctt ccagcgtcat cgccacgata
    27961 acgcctttcc cggctgccag accgtcagct ttaatgacga ttggcgcgcc tttctcacgc
    28021 agatacgcca gcgcaggttc tacctcggtg aagttctggt attccgctgt gggaatgtta
    28081 tggcgggcca gaaagtcttt ggtgaatgct ttagaacctt ccagttgtgc ggcacctgcg
    28141 gttgggccga agattttcag cccggcggcg cggaaggtat cgactacgcc tttcaccagc
    28201 ggcgcttccg ggccgacgat ggttagatcg atcttttcgt tttgcgcgaa atccagcagc
    28261 gccgggatat cggttacgcc gatagcgacg ttttgcagtg tgggttccag cgcagtgcct
    28321 gcattacccg gagcaacaaa aacagtttca accagcggcg actgggccgc tttccaggcc
    28381 agcgcgtgct cgcgcccgcc gttaccaatc actaatactt tcatctgtcg ctccattaat
    28441 ggcggaagtg gcgcatgtcg gtgaagagca tcgcaatacc gtgctcgtcg gcggcggcaa
    28501 tcacttcgtc atcacggata gaaccgccag gctggattac gcaggtcaca cccgcagcgg
    28561 cggcggcatc aataccgtcg cggaacggga agaacgcgtc agaagccatc gaggaacctt
    28621 tcacttccag gccttcatcg gccgctttaa taccggcgat ttttgcggag tacacgcggc
    28681 tcatctggcc cgcgccaatg ccgatggtca tattgttttt ggcatagaca atggcgttgg
    28741 atttcacgaa cttcgccact ttccagcaga acagcgcatc acgcagttcc tgttcggtcg
    28801 gctgacgttt ggtgacgacg cgcagttctt ctgcaccgac catgcccagg tcgcgatcct
    28861 gaaccagcag accgccgtta acacgtttga aatcaagacc cggaacacgc tcgccccact
    28921 gaccgcaggt caggacgcgt acgttctgtt tggcggcggt gattttcagg gcttcttcgc
    28981 tggcggacgg cgcaataatc acttcaacaa actgacgaga aatgatggcc tgcgcggttt
    29041 ccgcatccag ctcgcggtta aaggcgataa tgccgccgaa tgcggaggtc gggtcggttt
    29101 tgtacgcgcg atcgtaagca tcaagaatgg aattgctgat agccacgccg cacgggttgg
    29161 cgtgcttcac aatcacacat gctggctcgg cgaactcttt cacgcactcc agcgccgcat
    29221 cggtatcggc gatgttgtta taagagaggg ccttaccctg aacctgggtt gcggtggcaa
    29281 cagaggcttc tttcacgttc tcttctatat agaaggcagc ctgctggtgg ctgttctcgc
    29341 cgtaacgcat atcctgcttc ttaatgaagt tcaggttcag cgtgcgtggg aagcgaccgg
    29401 cggcttcttt gctttcaccg tggtaagccg gaaccatgct gccgaagtag ttggcaatca
    29461 tgctgtcgta ggcggcggta tgttcaaagg ctttgatagc gagatcgaaa cgggttgcaa
    29521 gcagaagcga tccttcgttg gcatccatct ctttaatgat ggcgtcgtag tcgctgctct
    29581 tcaccacgat ggcgacatct ttatggttct tggcggcaga gcgcaccatc gtcgggccgc
    29641 cgatatcgat gttctcaacc gcatcttcca gcgagcaacc ttcacgggca acggtctggg
    29701 cgaacggata caggttaaca accaccatat cgataggctg gatctgatgt tcttccataa
    29761 tggcatcgtc ctggccgcga cggcccagaa tgccaccatg tactttcgga tgcagggtct
    29821 tcacgcgtcc atccatcatc tccgggaaac cggtgtaatc ggaaacttcg gttaccggca
    29881 gacctttttc tgctaacaga cgggctgtgc cccctgtaga cagcagctcc acaccgcgtg
    29941 cggaaagtgc ctgggcgaat tcgacgatac cggctttgtc agaaacactg agcagagcgc
    30001 ggcggactgg acgacgttgt tgcatggtaa atcccctgga tttgactatt acagagagcg
    30061 ttagctgaat ttttcgcgaa aaactcagct aacgccccta acggggcatc cttatttttc
    30121 gcccgcattg taacgaaaac gtttgcgcaa cgctcgcgaa tttttctctt tcaatggtga
    30181 tcacaatttt gacagtggct accgtgggaa aaatacagaa attacattga tgattgtgga
    30241 taactctgtg cgtaaaaagg tataaagcgg ggttttgctg gggaatgcag cagtcagtca
    30301 tttttatgca atttttctat tgcgacctgc ggagaactcc ctataatgcg cctccatcga
    30361 cacggcggat gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaatcctga
    30421 aattcagggt tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgagct
    30481 gaaagccgcg tcgca
//
LOCUS       DAZKJR010000051        30431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKJR010000051 DAZKJR010000000
VERSION     DAZKJR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.051"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            200..457
                     /locus_tag="ACHOWS_002301"
     CDS             200..457
                     /locus_tag="ACHOWS_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0047866.1"
                     /translation="MTTQIATRGRLAVSLPCHGAEQPTTMWLYLITFGKQHEELAKHK
                     KGDLVQVHGNLQVNRYQDKFGQQKEGWQLLAQSIISSRTAR"
     gene            577..951
                     /locus_tag="ACHOWS_002302"
     CDS             577..951
                     /locus_tag="ACHOWS_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015834182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HFQ0047867.1"
                     /translation="MKHNDHGLNTLLDMHGYQHHMDNGYWWKIEAYRVSPNQFRPHGI
                     RYNLTLHDPYNSRVFGMDNAHGIKPPKKGKFTGRITVYDHIHRTSSDKGYPYEFTSAE
                     QLLLDFFNHVDRVIAEQQRGKK"
     gene            948..1325
                     /locus_tag="ACHOWS_002303"
     CDS             948..1325
                     /locus_tag="ACHOWS_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0047868.1"
                     /translation="MKARIGIIPEKVLRQRMLDIAKGKLKPEPDEPRVWFSSLNALGQ
                     ALSNENIALLRIMDAEKPQTMTELASLSGRQLSNLSATLKMLSRYGFVSLEKQGNCVH
                     PKALFTDFEIIIDPSVGETHRAA"
     gene            complement(2311..3357)
                     /locus_tag="ACHOWS_002304"
     CDS             complement(2311..3357)
                     /locus_tag="ACHOWS_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0047869.1"
                     /translation="MSKRKNYIGKRGQHSLPDALKSTPALSAFTFDGPYEVSRMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFIPHSLLSRQAFSA
                     FALDWFVFGNAYLEVRRNRLGEPLLLRPALAKYTRRGSDLDTYWYLNDDGTEFSFRKG
                     AVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIIYVGTPQA
                     NAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGIEVM
                     RFNPYALLEEGSAR"
     gene            complement(3363..5096)
                     /locus_tag="ACHOWS_002305"
     CDS             complement(3363..5096)
                     /locus_tag="ACHOWS_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFQ0047870.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDNW
                     DATPPIQRVTQSIDARLIQLTAKSDKTGGDFKEIDLLTRQLKKLNEGQGEQAVSGKKP
                     RKRKLKNHFTEEQIIALRERTLRTLACHQREWYENRHHRNRMILKSRQIGATWYFAHE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     ELHFLGTSAATAQSYTGHLKFDEFFWVSNFVQLRKVAGAMATLKGLTRTYFSTPSGET
                     HEAYQFWTGDRWNSERPRSKRTEFDVSHSNLKGGVLFPDKTWRQIVTIEDAVGNGWEY
                     TSIDDIRDENTESEFRNLYMCEFVRDGEAAFSLNALIGCGVDGYDEWPDWKPFAARPM
                     GVREVWLGYDANGSSGKGDCGALSVCVPPMVAGGRFRTVETVRVQGMEFEQQAALIEE
                     YTQKYNVKHIGIDVTGGNGEGVFQIVKKFFPMAVPYNFNIASKRALVMKMLQVVRAGR
                     WEYDRSERDLIQAFASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAE
                     REDNRGFAMEF"
     gene            5253..6089
                     /locus_tag="ACHOWS_002306"
     CDS             5253..6089
                     /locus_tag="ACHOWS_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFQ0047871.1"
                     /translation="MGSQKAPERKKFRVAVSGSTIDGREISGEMLRRAAENYDPSVYG
                     SRVNIEHALSLWPSSELCAMGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMLDE
                     GKKVYSSIELLPDLDAVQGPYIVGLAMTDTPASLGTERLKFAAQQRASIMRFNNRTSE
                     PSMFTAAMEAELLTTAEQQTEEGRQWFSRVMSLISKARGTDSEQFSQIREVVESIASD
                     HADLLNRFSALEKKHASGQETLKKLHDELAELKTQLKTLDADPQQRFIATGGNNQILA
                     EF"
     gene            6107..7192
                     /locus_tag="ACHOWS_002307"
     CDS             6107..7192
                     /locus_tag="ACHOWS_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFQ0047872.1"
                     /translation="MARLSLSREGRDSLERYMLQQAQLAGITTERLSKTFSVDPSIQQ
                     KMENAIKESAELLQKINVIGVDNQEGEKVLIDTSGPIASTNATSDGVKRRNPASVADL
                     DARRYRCEQVNYDTFISYAQIDAWSTQKNFQQLLSAQITRQIALDRIMIGFNGESHAL
                     ISDRAANPLLQDVNPGWLKHIRDKADTRIVKGMTLTRRDEENKLVSKGDYGNADAMVH
                     DIRSSVLDEWHKDAPDLVVLMGRDLFNTLRLPMINAISTTNPNTELVAGQLIVASRAI
                     GGLPTYLAPFFPKDAMLITSLSNLSIYFQKGSLRRYIREEPEYNRIATYQSMNDCYVV
                     EDYGKCALIEDISFAGEDPDEKPDSEE"
     gene            7258..8049
                     /gene="gpM"
                     /locus_tag="ACHOWS_002308"
     CDS             7258..8049
                     /gene="gpM"
                     /locus_tag="ACHOWS_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0047873.1"
                     /translation="MLTPAQRYVQQAVARNRVTMSTRETQRERTAHEQVLHQLRLAQQ
                     QLKGVQSNIARAELKRHLLPDFEGWIEGTLAGNSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLTEEIAEIVLTQAATNSDADLSGYTVMLDTLHGL
                     VHDQDMPDQVRAKLNKARAFSRRASTQPDDIHQSLMLLREAMRLNPAAGVKRDIATLE
                     RAVKKLSSPPPDPVKKSTKTTRKTGNSQKTSAKKKPSTRKPKNTH"
     gene            8159..8653
                     /locus_tag="ACHOWS_002309"
     CDS             8159..8653
                     /locus_tag="ACHOWS_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFQ0047874.1"
                     /translation="MSLIARTPAEHISEDIPDTDDGDSCVTADAFWPVIVLRELRLAV
                     RLPGRTTTARLMHAATEAVAHVTAELEMWKQERMKEGYTALADIPATHVNSESVHVHR
                     YRRAVYALARAFVLERTRDVDTTEKGDKRADSLDPQVEALWRDARWAISDIRGVTRIY
                     AELV"
     gene            8653..8853
                     /locus_tag="ACHOWS_002310"
     CDS             8653..8853
                     /locus_tag="ACHOWS_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFQ0047875.1"
                     /translation="MKIKALEGDTVDLLCFRHYGSTQGVTEQVLEANPGLCEQVFLVA
                     GQEVELPEQIREKKQETVLLWS"
     gene            8844..9140
                     /locus_tag="ACHOWS_002311"
     CDS             8844..9140
                     /locus_tag="ACHOWS_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0047876.1"
                     /translation="MELNIFQRIYDHITFSMSLMVTGIGVMTVSEKIAVAGLLLGALS
                     GLRAWIHRARLEKAQRRRNELIAQILAQSESRPLNASEKQALKQLEDQDDAPPH"
     gene            9124..9669
                     /locus_tag="ACHOWS_002312"
     CDS             9124..9669
                     /locus_tag="ACHOWS_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFQ0047877.1"
                     /translation="MPRHIKRYAVATIVALGVTLAPGALRTSEEAQVKIATWEDCRAR
                     PYYDTAGILTTGCGSTGNVKNRLYSENEVAERWVNDMQRAENCINKNFHGAAMPQSVF
                     EAMTDAAYNLGCTSLMWFKDRHGQRHRTTIWKHAQAHRWIQACARLTDFVNSGGKRIP
                     GLVNRRTDFKAWCLKDTGATS"
     gene            9666..10106
                     /locus_tag="ACHOWS_002313"
     CDS             9666..10106
                     /locus_tag="ACHOWS_002313"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFQ0047878.1"
                     /translation="MKPAGILFLMVLCMVAIVIAFRQTWRLGQESARNEALHETIRSN
                     SATLESIIAISRNTHALIAGLKVAEQHRNQEGEVRREHIRNAIRSDRCADTPVPAAIA
                     DSLQKRTARLSPELVRSSTGEPHHSHNATTADGASDMGKNRSME"
     gene            10213..12111
                     /locus_tag="ACHOWS_002314"
     CDS             10213..12111
                     /locus_tag="ACHOWS_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFQ0047879.1"
                     /translation="MKKYELITAMTCDELAKALDERLAEGWQPHGAPVAASEPGVPFY
                     LMQALIKSTDTATQGSNINTPTAEPEYYFVIPVAGQSNGMAYGEGLPLPETCDRPDPR
                     IKQLARRSTVTPGGESCRYNDIIPADHCLHDVQDMSGKNHPKADTSKGQYGTVGQGLH
                     IAKKLLPFLPSDAGILLVPCCRGGSAFTAGADGTYSDTTGASGNSARWGVDKPLYKDL
                     ISRTKAALAKNPKNRLLAVVWMQGEFDIDAKPTEHSALFLAMVEKFRTDLAEQAEQCT
                     GGSAAGVPWICGDTTYFWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGANVPTN
                     NPAEDPDIESIGYYGSTWRNSAATWTSADRESHFSSWARRGIISDRLATAILTHTVRA
                     AAVQPGASSSEPDVPETAATTTLLSYIASESEGNLKAQGWSASGGKAEIISDTGATGG
                     KAMKLTKQTGKGSWVLEHDAGTGDSLLQKGGLIRCRFKAQGELAANRYVMALYWPVSS
                     LPQNVTLAGNAGNNLLASFYIQTDAKDMNVMYHNAKAAKDNQKLGTFGAFDNEWHTLA
                     FRFAGNNSLQVTPVIDGKDGAPFTLTQSPVGMFTADKLHVTDVTKNATYPVLIDSIAV
                     EINNPVTA"
     gene            12264..12377
                     /locus_tag="ACHOWS_002315"
     CDS             12264..12377
                     /locus_tag="ACHOWS_002315"
                     /inference="COORDINATES: protein motif:HMM:NF018789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFQ0047880.1"
                     /translation="MDPVYTILLCFCTITCVLFLASGGGEILYQWCAKKTS"
     gene            12439..12909
                     /locus_tag="ACHOWS_002316"
     CDS             12439..12909
                     /locus_tag="ACHOWS_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0047881.1"
                     /translation="MLKMNALRDTLADTCRWCKANPDKFTVCVESGSIETTGESPSFL
                     YRYNLVIFAMDFTQDIDELFLPLLHWLYHNQPDLLLNPEKNSSIKFSASPNNDDSVDV
                     VIELPVWERVRVTRTPDRQVRAEHLQEPRPRIPSARGAWSNVYEDVTWSIPRDE"
     gene            12902..13549
                     /locus_tag="ACHOWS_002317"
     CDS             12902..13549
                     /locus_tag="ACHOWS_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFQ0047882.1"
                     /translation="MNEHKLDAVFRDILAGMSAAGLSRTARQVGQAVRRSQQQRMRAQ
                     KAPDDTPWAPRKRQIQRVQQGIKFLWQPPAAGSQPMVRELKNWKHGTGRHGPHITGYD
                     INRGGLRTFYRADIEEYLEIRTRRVSQGNTVRARMFERLRRYVKILQADNESIRVGFN
                     GDQARIARVHHFGLRDNVVPGVTTQYPARELLGLSVKDKAMIRETIIGSLKGGMK"
     gene            13546..14127
                     /locus_tag="ACHOWS_002318"
     CDS             13546..14127
                     /locus_tag="ACHOWS_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFQ0047883.1"
                     /translation="MSAEIMRLLENMIRVGVISDVDLKNWRVRVASGRLTTDWLRWNC
                     TRAGVFKIWLPPSRGEQVLLLCPGGNPEMAMVVGSLYSTDNPAPGTQQDEVIITARDG
                     ARLRYNAKEGALDATGIKTARVTAETRITLETPLVECTQHLKTRTFELTDGGTLKGDV
                     THSGGSLTSNGVVTHTHTHSGVQSGGSQTGGPQ"
     gene            14124..14474
                     /locus_tag="ACHOWS_002319"
     CDS             14124..14474
                     /locus_tag="ACHOWS_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFQ0047884.1"
                     /translation="MTATYTGMNPYHPGVVHDTEHLHCSVRDILLTPQGSRIMRRDYG
                     SLVPDLLDAPQNDVTRLQCMSAAVIALTRHEPRLALNTIDVRWLDEGGAELLLTGIIT
                     ATMQTVRLSIRTGD"
     gene            14478..15374
                     /locus_tag="ACHOWS_002320"
     CDS             14478..15374
                     /locus_tag="ACHOWS_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFQ0047885.1"
                     /translation="MPTVDLSQLPKPAIINTPDFEVILNEIKTMMIGCFPEEQQPAVT
                     AALALESEPLTIIAEVMAFREMLLRQRINEGVAACMLSHASGTDLDHLAANLNTQRLV
                     ITPATDTAPAVMENDTALRLRAQSAYEGLSVAGPSASYEYFARSASGQVADARAVSPS
                     PAVVVVSILSTEGDGTASQELIQTVSNALNDERIRPVADRLTVQSAKIVKYRINARLF
                     LYPGPESEPVILAARQSLDEWLTEQGHIGRDVARSAIMSALHVQGVQRVELEEPASNI
                     AVDDSQFARCESIIVDMGGTNE"
     gene            15367..15909
                     /locus_tag="ACHOWS_002321"
     CDS             15367..15909
                     /locus_tag="ACHOWS_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFQ0047886.1"
                     /translation="MSDSLLPPSASDFLKYTGYATDRITRIPVDLRTLWDPEACPVEL
                     LPYLAWALSVERWDKNWPEETKRKVIRESWLIHRHKGTFSSLRRIVEPLGYIISMSEW
                     WETGDPPGTFRLNVGVFEGGLTEEMYFEIERLIADARPVSRHLVGLNIVQDISGKVYT
                     GGMVYDGDIITVYPIQESDA"
     gene            15906..17567
                     /locus_tag="ACHOWS_002322"
     CDS             15906..17567
                     /locus_tag="ACHOWS_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0047887.1"
                     /translation="MSDTVKFKTIITTAGAEKLAASASDGGKKVTPAVMAVGDGGGTL
                     PDPDAKQTALINEVWRHDLNRVFIDNTSKNTVVAELIIPAETGGFWMRELGLYDDEGT
                     LIAVANMAESYKPRIAEGTGRSQICRMVIAVSNIETISLTVDSTVVTATKEYVDNLLE
                     EHEKSRNHPRASQAAPGLVQLSSQTDLDSEIMAATPKAIKIVMENANGRLAKNRNGGD
                     IPNKELFLINVGAPRVYAKDGYVGEAGQRWTTEQFVEWLNERGAFRPGLWIFRANISL
                     SRHSVLTDTGYGDICLGGCLIEVSGDINGGIIRITTGLESPNPADMYLRTQFTGVVLN
                     KGKSTIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPWPSDTPPGGYAIMAGQTFDK
                     ARYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHGGSVSNTDLGTK
                     TTSSFDYGTKTTSTNGQHEHSVPLRRQDGGGINFDWLDGSSSGSHIGNGYVPAAGNHQ
                     HTVPIGAHTHTLALGSHGHGLTINPTGNAENTVKNIAFNYIVRLA"
     gene            17567..18292
                     /locus_tag="ACHOWS_002323"
     CDS             17567..18292
                     /locus_tag="ACHOWS_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFQ0047888.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENAWHLMEDHRGKTVYHEETGEKLVITGPGPLPSSVTTEPP
                     GTSADVYRDGQWVTDLSAARGIKNSEINDWRNRMENAGYIMEYNGRKWDYGKATQDRL
                     EPSVTAARAGKLPAEFFWTDAENNDVPMSADELIALSEAVGEAMFKKGLEIHIRQREM
                     KKELAGMDNAGEIMKFTPGWKIT"
     gene            complement(18335..18823)
                     /locus_tag="ACHOWS_002324"
     CDS             complement(18335..18823)
                     /locus_tag="ACHOWS_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0047889.1"
                     /translation="MMMIYGMFVFQLSTLPHQQLQQSRGWRHVSNERINRSAGWQYIG
                     AGEDKITLSGVLYPEITGGEVSLTVLNTQAYTGRPWPLIDGAGQIYGMYVITDIQTTR
                     EEFDRYGRAKKITFSVSFRRCDEDLRERLQASSVGDLLNNLKDAASAAGERVSSMASG
                     IL"
     gene            complement(18846..21641)
                     /locus_tag="ACHOWS_002325"
     CDS             complement(18846..21641)
                     /locus_tag="ACHOWS_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0047890.1"
                     /translation="MNTLDIRVAFSAVDNLTRPATSARESAEALAASLRNTQNNLRDL
                     ERQSRSFSSARKHLTEVTDKIKRAQQELNGLQRLQQSGRPLTEAQQELINKLTARLER
                     LNEVRTQERNRLREAGQALRRHGVTLSGGDRAIEAAIRRTQQYNEELERQRLALARVS
                     RARDEYTRAMAVSEKLRGAGTAMMLAGLGGMYAGSRMVAPVMNEERYGAVIAAQTGEG
                     RAEASGYTRLVRELHHAGIAGDAARAAEAVGAVRSTLGALGVTGDTEISRITRRMMDM
                     QTVMGVDVPQGIQAAAIMMKNGLARSSDEALDLMVSAMQRMGPEMRGELPEIIHEYST
                     HFRNLGFSGAEAMSLLADMAGQGKFALDKTGDAVKEFSIRGSDMSKSSVEAYESIGLN
                     AAQMSSAIASGGVAARDAMQKTAAGLLRIRDPAERANAAIALFGTPVEDLAVDQIPAF
                     LGALARTSVVLGNTEGAAERMGNTLRDNLSGDVLKLTGSLAGLRADAVSTVTETLRGL
                     VQTATQWTARMREWVQKNPELVRGLLMVAGVVAGLSAAIGGLLVTVGMVLIPLVKLRL
                     GLSLIRALPAGISVVSGLFGGLGGVISSLGTAVGMLTGPVGLVVAALLGAGLLIYKYW
                     DQLRAFVLGFIDGARGALAPLLATVSRFAPLLDLVAGAVSKVFLWFKSLLSPVAATRD
                     TLDKCTAAGARFGSSLVSALTLVFSPMKALIDGLAWVLEKLGVLPGEAARVKQKLDAA
                     GKTPVVWEWDPEQKKMVQKAWNWSPKGAGGTQSVGVLRLPEMPAVLQPSANSGNGGVL
                     DRLKGIEGNTRDIVRETKKRTGPGDIIFRNLPPALVVRGQWTEPRLAGSTGVSGARIE
                     EHQNIKVASPPPLTVPSSGSERGLSSVGFSGEIHVHLHNVVTQDPRTLARMIGEAVKA
                     ELSGQSRNGLRSFRDVD"
     gene            complement(21638..21784)
                     /locus_tag="ACHOWS_002326"
     CDS             complement(21638..21784)
                     /locus_tag="ACHOWS_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFQ0047891.1"
                     /translation="MQFEHIEDVVADIAVVFNWSPADLFMMGPEDVIAWRKRAADRNG
                     SPEK"
     gene            complement(21793..22164)
                     /locus_tag="ACHOWS_002327"
     CDS             complement(21793..22164)
                     /locus_tag="ACHOWS_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047892.1"
                     /translation="MNENAKLTKTAELVTDKDIGSNASPDVVSLITPVVRDTETIKSV
                     KIGPLARQAGSLRGLSLVDLYGMKFDALLTYLSRVTEPRLKESELKAMDARDFSALAN
                     AAVNFYVGGVSTKPNGEETDG"
     gene            complement(22206..22718)
                     /locus_tag="ACHOWS_002328"
     CDS             complement(22206..22718)
                     /locus_tag="ACHOWS_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFQ0047893.1"
                     /translation="MAVPHKLRFMTLFIDGKNEIGQVVSVTLPKLTRKTESYRGGGML
                     GSVAVDLGLDDSALDASFVMGGAAADLVRKYAGRIDEVRLRFAGEYYTDGDSQLIEVE
                     MRGRISEIDMGEAKQGEDTQHTYNVKNTYYKLSSDDRELLEFDLLNFIYRQDGENIVP
                     DRIRSALGMG"
     gene            complement(22718..23905)
                     /locus_tag="ACHOWS_002329"
     CDS             complement(22718..23905)
                     /locus_tag="ACHOWS_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFQ0047894.1"
                     /translation="MSETRFHGARVRESTDLVTAINDIDSSVIGIVAVADDADTGTFP
                     LDKPVLLTRVNDVLGKAGKAGTLYKALKAISDQVSTKVIVVRVAAEKKGDKQKTQDQL
                     VIGAVEADGRYTGMQALLVAEQDEAIGYRPRILAAPGLDTKAVTTALCTIAGKLRAFV
                     YAGCPGCNTATEAMTWRQDLAYRELMLIWPDFIAYNPQSGKNESFPSAAYACGLRASL
                     DHEQGWHKSLSNEPVKNVLGVSHPVYWSLQSEDSDANNLNNKEITTIIRRNGFRFWGN
                     RTTDTSAYIFEVYTRTAQTLADTIAEAQFEVVDDPLTPANVKDAISAIRAKLNELVTA
                     GKLIGAECWYDVIDNSTTDLRQGRVRIRYKYTPVPPLEDLTLHQTFTDEFFGPAFASL
                     GGA"
     gene            24067..25194
                     /locus_tag="ACHOWS_002330"
     CDS             24067..25194
                     /locus_tag="ACHOWS_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFQ0047895.1"
                     /translation="MADRIIRIIKNYDDRTSTLVKLPDFSITLNNNELEAQELKNRVM
                     SLSVTDNRGFEADQISLTLDDTDGQLILPERGAVLSIAIGWKGESLTDKGTYIVDEVS
                     HDGPPDKLIIGASSADFREEFNVKREVSWHDVTLERVVSAIAHRYGLKAQISEMLMHI
                     EIDHADQTEESDMSFLTRMADMLGAIATVKNGNLLFIIPGGGVTASGKPLPSFTLTRS
                     DGDQHRFRIADRQAYTGVRAYWLDLNFGKKKQVSVKKRSLSQEKSHSREGDYMEGAEG
                     NVFVLRRTFPNEESAKRAAAAKWQQLQKGAAEFAITLAHGRADLYPEMHGTVSGFKTT
                     IDRQDWIIARVEHTIDSNGFTTQLELEAKIPDWIAESEQSG"
     gene            25238..25489
                     /locus_tag="ACHOWS_002331"
     CDS             25238..25489
                     /locus_tag="ACHOWS_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFQ0047896.1"
                     /translation="MFYCPHCGASARIRTSKAANDTGTIRYKYYQCNNIECGATFSTM
                     ESVHKMISVPTPSGERDEQIPHEHFPATHRGRLQLILEL"
     gene            25675..25815
                     /locus_tag="ACHOWS_002332"
     CDS             25675..25815
                     /locus_tag="ACHOWS_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFQ0047897.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHNSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            26144..26284
                     /locus_tag="ACHOWS_002333"
     CDS             26144..26284
                     /locus_tag="ACHOWS_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFQ0047898.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSLWGAEFAAFLQC
                     KQ"
     gene            complement(26643..26729)
                     /locus_tag="ACHOWS_002334"
     tRNA            complement(26643..26729)
                     /locus_tag="ACHOWS_002334"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26693..26695),aa:Leu,seq:taa)
     gene            complement(26742..26815)
                     /locus_tag="ACHOWS_002335"
     tRNA            complement(26742..26815)
                     /locus_tag="ACHOWS_002335"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26781..26783),aa:Cys,seq:gca)
     gene            complement(26870..26945)
                     /locus_tag="ACHOWS_002336"
     tRNA            complement(26870..26945)
                     /locus_tag="ACHOWS_002336"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26910..26912),aa:Gly,seq:gcc)
     gene            complement(27097..27645)
                     /gene="pgsA"
                     /locus_tag="ACHOWS_002337"
     CDS             complement(27097..27645)
                     /gene="pgsA"
                     /locus_tag="ACHOWS_002337"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0047899.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(27702..29534)
                     /gene="uvrC"
                     /locus_tag="ACHOWS_002338"
     CDS             complement(27702..29534)
                     /gene="uvrC"
                     /locus_tag="ACHOWS_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0047900.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(29531..30187)
                     /gene="uvrY"
                     /locus_tag="ACHOWS_002339"
     CDS             complement(29531..30187)
                     /gene="uvrY"
                     /locus_tag="ACHOWS_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFQ0047901.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         7985 a         8317 c         7625 g         6504 t
ORIGIN      
        1 acaagataac gcattaccgg aagccaacgg aaaaggaacg acaggaactg gcgcaacggg
       61 ctgaacggca gcaggaaaaa tcagtcatcg aagcctgcaa gcgcagcgaa atcatgaagc
      121 gaattctgtt tatctacggc gctggcggag atctgccagt catatcagcc caataactaa
      181 accaccaacg gagacaaata tgacaacgca gattgcaaca cggggaagac tggcggtatc
      241 actgccctgc catggcgcag aacagccaac cacaatgtgg ctttacctta tcacattcgg
      301 gaagcagcac gaagaactgg caaaacacaa aaaaggcgat ctggtacagg tgcacgggaa
      361 cttgcaggta aacaggtatc aggacaagtt cggacaacag aaagagggct ggcaactgct
      421 ggcacaaagc attatcagct caagaaccgc gcgatgactt cacgcatgtt accctcactg
      481 ttgagggtaa ctacgacgac cgcacagaca acccagcttg ataaagaaaa aaatgatgtt
      541 atgattaagt cataagtaag gtgatacgaa tcagttatga aacacaatga tcatgggctg
      601 aacaccctcc tggatatgca tggataccag caccacatgg ataatggtta ctggtggaag
      661 attgaagcat acagggttag cccaaaccag ttcaggccac atggaatacg ctacaacctg
      721 acattgcacg atccttataa ctcccgggta tttggaatgg ataacgccca cggaataaag
      781 ccaccaaaga aagggaaatt taccgggcgc attaccgttt atgaccacat tcacagaaca
      841 tcgtcagata aaggctatcc atatgaattc acttctgcgg aacagctttt actggatttc
      901 tttaaccatg tagacagagt gattgctgaa cagcaaaggg ggaagaaatg aaagcccgta
      961 tcggcattat cccggagaaa gtccttcgcc agagaatgct ggatatagcc aaagggaaac
     1021 taaaaccaga acctgacgaa ccgcgagtct ggttttcatc tctgaacgct ctgggtcagg
     1081 cgctgagcaa cgaaaatatc gcgcttctgc gtatcatgga tgcggaaaaa ccgcagacaa
     1141 tgacagaact tgccagtctt tcgggaaggc agttaagtaa tttgtcagca acgttaaaaa
     1201 tgctgagtcg atacggcttt gtcagcctgg aaaaacaagg taactgcgta cacccgaaag
     1261 cgttgttcac ggattttgaa atcataatag acccgtcagt aggtgaaact catcgggcag
     1321 cgtgaatatt gcgggggtaa tccccgctca tactttttta gcctgcgtca cccgcctgaa
     1381 tacccacctg aaaaaatgca caaaagtgca cgaaattgca caatttttct tatgtatttt
     1441 ttccccattc agcccggtaa cggcgcgggc tggggccagt ttgcacggtg cacgaaaaat
     1501 gaggcaattg cagcgcgcag gtggcgtggg acagggcgca cgaattgagg ggtggaaggc
     1561 aacttgcttt atttggggcc gtattgcgcg tgatacgcac acagtgaagc cagttttgaa
     1621 aaaccacatc aaaacatagc cagagaaaca gtccgtcaga atgcgtctgg ttgcgtctgg
     1681 agaggtcggg gaagccagag aaaaagcgag cgtcgaacgt gatgaaaaag agggataaaa
     1741 atcaccggaa tcataaagtt aagagcattt aaagtatcca ggatccacgg gtgaaaacac
     1801 acgcacaata tgaggtgaac gcagagtgca ggcgggcatc aacagaccga aggaggacgg
     1861 tatgaaccat tttcgcgact ggtgtcaggc aaaccagaag agccaggacg cgagacggga
     1921 ggcgctctgt attctccgcc gccagtccag gctgcaaaga aaagcgcctc cgcattccgt
     1981 taagagagct ggtgagtcgg ggcataaatt ttttatgccc cggcgaagca gcagacaagc
     2041 gaagcgcgtc agttggtatt cgtactatcc ggccatcacc cgaacaggac gaacaccacg
     2101 acgtgttaca cgagtaccgg agacagtctg gtgattctgg cccttaatcc ggtaatctag
     2161 ggctgacctg atataaaact gacacaagtc tggtacaaac tggatttgga ttagatttaa
     2221 tttgaattaa atttggatct gttttgtaga taattttgat ctgaaccatc gcgcattttg
     2281 tgaatggatg cagaccattg ccagcgagcc ttaccgggcg ctgccctcct ccagaagtgc
     2341 atagggatta aagcgcatca cctcgatccc cagccattca ttgacatgct tcatggcctc
     2401 cataacaggt tgcagctcgt tgatgaaaaa caccctcgcc gctttctcaa cgtcaccgaa
     2461 cgccgcgttg ccatcaggca tggcccccat cagttgaggc ggtacacgat gggccgccag
     2521 gatatcgtcg cgggcactcc ctttgatgtt caggaattca tcctttgcgg ttatctgctg
     2581 gaatggcaga atttgcaccc catccttgcc gccacccggc gcatgaagaa acaggttttt
     2641 aaacgccccg cgaccacgcg aatccgtgag cgttttttta atagcctcga cactttccgc
     2701 gttcgcctga ggcgtaccaa catagataat acatccagca tgcgagccat tatcgtagta
     2761 gagcttgcgg aacgtatccg ctgaatttga caggctgacg gacagcagcc caccaatata
     2821 ttccggcatc ccgtagatct cctgattaat atcaggatta agcacgtggc acacagcccc
     2881 tttccggaaa ctgaactccg taccgtcgtc gttgagatac cagtaggtat ccaggtcact
     2941 tcccctgcgt gtatattttg ccagtgccgg gcgaagcagg agtggttccc ccagcctgtt
     3001 acgtcgtact tcaaggtagg cattgccaaa cacaaaccag tcaagcgcaa acgcggaaaa
     3061 cgcctgccgg gaaagcagag aatgcggaat aaagcaccca ttcagggcgt tgcgcttgaa
     3121 gtacaaagcc gactgatgcc aagacgcacg acgggatgca cgggcgatag cgttccagtc
     3181 catgggggta tcaaagtagc gcccgttatc tgcacatgac atgttatcca ggagatccat
     3241 gcggctgact tcatagggtc catcaaaggt gaatgcactg agtgccggtg ttgatttcag
     3301 tgcatcgggt agcgaatgct gcccacgctt tccgatgtaa tttttgcgtt tactcattgt
     3361 gttcagaact ccattgcaaa gccacggtta tcctcccgct ctgcaccgat tggctcatta
     3421 atcacggcaa gcattgttgc ccaggcgagg tcaccatggc tgacaccacg ggagcggtcg
     3481 gtgtcatagg tgatatagcc gccgggtgtc tgtatttttc gcaccgatgc gaatgcctgg
     3541 atcaggtcac gttcactgcg gtcatactcc cagcgtccgg cacgcaccac ctgaagcatt
     3601 ttcatgacca gtgcccgctt ggaggcgata ttgaagttgt acggcacggc catcgggaaa
     3661 aactttttca caatctggaa taccccttca ccgttcccgc cggtcacatc aataccgata
     3721 tgtttcacgt tatatttttg ggtgtattcc tcaatcaggg ccgcctgctg ttcaaactcc
     3781 ataccctgta ccctgaccgt ctccacggta cgaaaacgcc cgcctgcaac catcggtggt
     3841 acacatactg acaatgcacc acagtctccc ttaccgctac tgccgttggc gtcgtacccg
     3901 agccagactt cccgcacgcc catcggacgc gcagcaaaag gtttccagtc aggccattcg
     3961 tcgtatccat ccacaccaca gccgatcagc gcattcagac tgaaagccgc ctcgccatca
     4021 cgcacgaact cgcacatgta caggttacga aattcgcttt cggtgttttc atcccggata
     4081 tcgtcaatgc tcgtatattc ccacccgttc ccgactgcat cctcaatggt gacaatctgc
     4141 cgccatgttt tatccgggaa gagcacgccc cctttcaggt tcgaatgaga cacatcgaat
     4201 tcagtgcgtt ttgagcgggg acgctcactg ttccagcgat cccccgtcca gaactgataa
     4261 gcttcatgcg tttcacccga tggcgttgaa aagtaggtac gggtaagccc tttcagggtc
     4321 gccattgcgc cagccacctt tcgcaactgc acaaaattac tgacccagaa aaattcatca
     4381 aatttaaggt gtccggtata cgactgagcg gttgctgccg atgtgccaag gaagtgaagc
     4441 tctgcaccgt tagacaaaac aatcttgtca ccccctttca gttcgacatc aacttccagc
     4501 gccgcctgct gaataaagtg tttgaactga tacgcctggc gacgggatgc ggacaaaaat
     4561 atctggttgc gctgataccc ggcagagaca tcatcacgca gcgcatcaag gagcgcctcg
     4621 tgcgcaaaat accaggtagc cccaatctga cgcgacttca ggatcatacg attacggtgg
     4681 tggcggtttt cataccattc acgctgatgg caggccagtg ttcggagggt tctttcacgc
     4741 agggcgatga tttgctcttc cgtaaagtga tttttcagtt tgcgcttgcg gggcttctta
     4801 ccggaaacag cctgttcacc ctgcccctcg ttcagttttt tcagctgacg cgtcagcagg
     4861 tcaatttcct taaaatcgcc ccccgtctta tcggacttgg cggtaagctg gatcagtcgc
     4921 gcatcgatgg actgagtgac acgctggatc gggggcgtgg catcccagtt atcccgtttt
     4981 ttccatgaat aaatcgtgtt ctggttaatc cccatcagtt gagcgatatc tgccggagtg
     5041 tacccctgcc agtaaagctg tttcgcccgc atacggataa atacatcctg gatcattgcg
     5101 ccccctgcca tttacacggc aagattaaaa cctccttaac acctcctgat acctgccgct
     5161 gttgtgcctg gcgccataca acttcaccac cttgagacac tcccccgtac accgccatca
     5221 tgagtaaaac aactctgcta tggagaattt ctatgggaag tcaaaaggcg ccggagcgaa
     5281 aaaaatttcg cgttgccgta tccggttcaa ccattgacgg acgcgagatt tcgggggaaa
     5341 tgctgcgccg cgctgcagaa aactacgacc cgtccgttta cggctcacgg gttaatatcg
     5401 agcacgctct ttcgctgtgg ccctccagtg agctctgcgc gatgggcgat ctgatcgcac
     5461 tgtcaaccga tgaaatcacc gaaggaaaac tggccgggcg cacagcgtta tatgccgaga
     5521 ttgaacccac cgatcgcatg aaaaacatgc tcgatgaagg gaagaaagta tattcaagca
     5581 ttgaactgtt accggacctt gacgccgttc aggggcctta cattgtaggc ctggcaatga
     5641 ctgatacacc ggcaagcctg ggaactgagc gcctgaaatt tgccgcacag cagcgcgcca
     5701 gcatcatgcg cttcaataac cgtacaagtg aaccctccat gttcaccgca gccatggaag
     5761 ccgaactgct taccaccgca gagcagcaga cagaggaagg ccgccagtgg ttctctcgtg
     5821 tcatgtctct catcagcaaa gccagaggga cagacagcga gcagttcagc cagatacggg
     5881 aagtggtgga aagtatcgcc tccgatcatg ccgacctgct caaccgcttc agcgctcttg
     5941 agaagaaaca cgccagcgga caggaaacac tgaaaaaact gcacgacgaa ctggcagaac
     6001 tgaagacgca actgaaaacc cttgatgctg atccgcagca acgttttatt gccaccggtg
     6061 gtaataacca gattctggct gaattctgaa aggagaagat taacttatgg cccgtttatc
     6121 cctttcccgt gaaggacgtg acagtctgga gcgctacatg cttcagcagg cgcagcttgc
     6181 tggcatcacc acagaacggc tgagcaaaac attcagcgtt gatccgtcaa tccagcagaa
     6241 gatggagaac gccatcaagg aaagcgccga actgctgcag aaaattaatg ttatcggtgt
     6301 tgataaccag gaaggtgaaa aagtcctgat tgacaccagt ggccccatcg ccagcacgaa
     6361 cgcaacgagc gacggagtga aacgccgtaa tcctgcatca gttgccgacc tagatgcgcg
     6421 ccgttaccgt tgcgaacagg tgaactacga taccttcatc agttacgcac agattgatgc
     6481 gtggagcacc cagaagaact ttcagcaact actgagtgca caaatcaccc gtcagatcgc
     6541 ccttgaccgt atcatgattg gcttcaacgg cgagtcgcat gcactgattt cagatcgcgc
     6601 agctaaccct ctcctgcagg acgtcaaccc cggctggctg aaacatatcc gcgacaaggc
     6661 agacacccgc atagtgaagg ggatgaccct gacccgtcgc gatgaagaaa acaaactggt
     6721 atcaaaaggt gactatggta atgccgatgc catggtgcac gacatccgct catctgttct
     6781 ggatgaatgg cacaaggacg cgccggatct ggttgtactg atgggacgcg acctgttcaa
     6841 tacactgcgc ctgccgatga ttaatgccat cagcaccacc aacccgaaca ctgaactggt
     6901 tgccggacag ttgattgtgg catcccgtgc catcggcggt ctgccgacgt atctcgcacc
     6961 gtttttcccg aaagacgcca tgctcatcac ctcactgagc aacctgtcga tctacttcca
     7021 gaaaggttca ctgcgccgtt atatccgcga agagccggaa tacaaccgga ttgccactta
     7081 tcagtccatg aatgactgct acgtggtgga agactacggc aaatgtgcac tgattgagga
     7141 tatttccttt gccggtgaag atccggatga gaagccggac agcgaggaat gacgacctga
     7201 agcaggcgca caggcataac cggggcgcat acgcgcccgt aaatcaggag ctgaaggatg
     7261 ctgacaccgg cacaacgtta tgtgcaacag gcggtggccc gtaaccgggt aaccatgagt
     7321 acacgagaaa cgcagcggga aagaaccgca catgagcagg ttcttcacca gttaaggctg
     7381 gcacagcaac aactgaaggg tgttcaatca aacatcgcca gagcagaact caaaagacac
     7441 ctgctgcctg actttgaggg atggatagag ggaaccctgg ccggaaacag cggtcgccag
     7501 gacgaggtta tcaccacaat gatggtatgg gcgatcgact gccgtgacta cccgctggcg
     7561 ctgcgcatcg gggaatacgt gatccgtcat ggtctggccc tcccggataa ctttggtcgc
     7621 gatgccgcca ctgtactcac agaggaaatt gccgaaatcg tcctgacaca ggccgccaca
     7681 aacagcgatg cagatttatc aggctatacc gtcatgcttg atacgctaca cgggctggta
     7741 catgaccagg atatgccgga tcaggttcgg gccaaactga acaaagcccg cgctttttcc
     7801 cgcagggcat ccactcagcc tgatgacatc catcagtcac tgatgctgtt gcgggaagcc
     7861 atgcgcctga atccggccgc aggcgtgaaa cgggacattg ccactctgga aagagccgtc
     7921 aaaaagctgt cttcgccccc tccggatccg gtcaaaaaaa gcaccaaaac caccaggaaa
     7981 accggcaaca gtcaaaaaac cagtgcgaag aaaaagccat ccaccagaaa gcccaaaaac
     8041 acgcactgat ttaaacgaac tgacccacgt cagcaggcgg cgcaaccggc gatctgtatg
     8101 ttttacacat gctttttacc ggttgcccac cgcctgattt ttacggagag agttttgtat
     8161 gagcctgata gcacggacac cggcagagca catcagtgaa gacattcctg atacagatga
     8221 cggagacagc tgtgtgaccg ctgacgcatt ctggcctgtc attgtattac gggagctgag
     8281 actggctgtt cggcttcctg ggcgaacaac cacggcgcgc ctgatgcatg ccgccacaga
     8341 ggccgtcgct cacgttaccg ccgaactgga gatgtggaaa caggaacgga tgaaagaagg
     8401 ttacaccgcc ctggcagata ttccggcaac gcatgttaac agtgaaagtg tgcatgtcca
     8461 ccgctaccgc agggcggtgt atgcccttgc ccgtgcattt gttctcgaac gtacacggga
     8521 tgtggacacc acagaaaaag gtgacaaaag ggctgactca ctcgacccac aggttgaagc
     8581 tctctggcgg gatgcccggt gggcaatttc agatatacgc ggcgtcactc gcatttatgc
     8641 ggagcttgtc tgatgaaaat caaagcgctg gaaggtgaca ccgttgatct gctctgtttt
     8701 cgtcactacg gcagtacaca gggcgtcaca gagcaggtgc ttgaagccaa tccgggatta
     8761 tgtgagcagg tttttctggt tgcaggccag gaagtggaac tgccggagca aatccgtgag
     8821 aaaaaacagg aaacggtatt gctatggagc tgaatatttt tcagagaatt tacgaccaca
     8881 tcaccttctc gatgtcactc atggttacag gcatcggtgt gatgacggtc agcgagaaga
     8941 tagcagttgc cggtcttctg ctgggcgcgc tgtccggtct tcgcgcatgg atccacaggg
     9001 cgcggctgga aaaagcgcaa cgacgccgca atgaactgat agcacaaatc cttgcgcagt
     9061 cagaatcgcg ccctctgaac gcatcagaaa aacaggcgct gaagcaactg gaggatcagg
     9121 acgatgcccc gccacattaa gcgctatgcc gtagccacca tcgtggcgct gggcgtgact
     9181 ctggcaccgg gcgccctgcg aacctctgaa gaagcgcagg taaaaatcgc cacatgggaa
     9241 gactgtcggg cccggcctta ctatgacacg gccggcattc ttaccactgg ctgcggctca
     9301 acaggaaacg tgaaaaaccg gttatacagt gaaaatgagg tggctgagcg gtgggttaac
     9361 gacatgcagc gcgcagagaa ctgtatcaat aaaaacttcc acggtgccgc catgcctcag
     9421 tctgtgtttg aggctatgac ggatgccgcc tataacctgg gctgtaccag tctgatgtgg
     9481 tttaaagaca ggcacgggca gcgtcaccgg acaacgatat ggaagcatgc acaggcccac
     9541 agatggatcc aggcatgtgc acgtctgacc gattttgtga acagtggcgg caaaaggatc
     9601 cccggactgg ttaaccggcg cacagacttt aaggcatggt gcctgaagga cacaggggct
     9661 acatcatgaa accggcaggc atcctctttc tgatggtctt atgtatggtc gcgattgtca
     9721 tcgcattccg gcagacatgg cgactggggc aggaaagcgc acgtaatgag gctctccatg
     9781 aaaccatcag aagtaacagc gccacactgg aatccatcat agctatcagc cgcaacactc
     9841 acgcgctcat tgccggtctg aaggtggcag aacaacatcg taatcaggaa ggagaagtca
     9901 gacgtgaaca tatccgcaat gccatccgca gcgatcggtg cgctgacact cccgtgcctg
     9961 ctgctattgc tgacagcctg caaaaacgta ccgcccgtct ctccccggag ctggtgcgaa
    10021 gcagtaccgg agagcctcac cacagtcaca atgccaccac cgctgacgga gccagtgaca
    10081 tggggaaaaa tcgcagtatg gagtgacagc ctgctggatg cactggacag ctgtaacgca
    10141 gataaagcag ccatccgaca actgaacgct caacagatgg caacaagaga taacgagtaa
    10201 ccggagtaca gtatgaaaaa atacgaactg atcacagcca tgacgtgtga tgagctggca
    10261 aaagcactgg atgaacgcct ggctgaaggc tggcaaccgc acggagcccc cgtggcagca
    10321 tcagaaccgg gcgttccctt ctatctgatg caggcgctga ttaaatccac ggacactgca
    10381 acgcaaggca gcaacatcaa cacacccacg gcagaaccgg agtattactt tgtgatccct
    10441 gttgccgggc agtcaaacgg catggcatac ggcgaaggtc tgccattgcc cgaaacctgt
    10501 gaccgtccgg acccgcgaat taagcaactg gcgcgtcgca gtacggtaac tccgggcggc
    10561 gagtcctgca ggtataacga catcatcccg gcagatcact gcctgcatga tgtacaggac
    10621 atgagcggga aaaatcaccc gaaagcagac acatcgaaag gacagtacgg cactgtaggt
    10681 caggggctgc acatcgccaa aaaactgctg ccgttcctgc catctgatgc gggaattctg
    10741 cttgtccctt gctgccgtgg cggttccgca ttcacggcag gcgctgacgg tacgtacagt
    10801 gacactactg gcgcctcagg gaattcagcc cgctggggtg tggataagcc gctgtataag
    10861 gacttaatca gccggacaaa agcggcactg gcgaagaacc cgaaaaaccg cctgcttgcc
    10921 gtggtgtgga tgcagggcga gtttgatatt gatgcgaagc cgacggagca ttccgcgctg
    10981 tttctggcga tggtggaaaa attccgcaca gacctggctg aacaggcgga acagtgtacc
    11041 ggtggcagtg ctgctggcgt tccgtggatt tgtggcgata ccacgtattt ctggaaacag
    11101 aaaaacgaac cggcatacca ggcaatctat ggcggctaca aaaataaaac agataaaaac
    11161 attcatttcg tcccattaat gacagatgaa aatggcgcga atgtacccac caacaatccg
    11221 gcagaagacc cggatattga atccattggt tattacggct caacgtggcg taacagcgct
    11281 gccacctgga catctgcgga ccgggaaagc cacttcagtt cgtgggcacg cagggggatc
    11341 atttcagacc gccttgccac agccattctg acccatactg tccgggctgc tgcagtacag
    11401 cctggtgcat catcttcaga acctgatgta ccggaaaccg cagccaccac cacgttactg
    11461 tcatatatcg ccagtgaatc agaaggaaac ctgaaagcac agggctggtc ggccagtggc
    11521 ggtaaagcag aaatcatcag cgataccgga gccacgggcg gtaaggcaat gaaactgacc
    11581 aaacaaaccg gcaaaggatc ctgggttctg gagcatgacg caggcacggg tgacagtctg
    11641 ttacagaaag gcggtttaat tcgttgtcgc tttaaagcgc agggcgaact ggcggcaaac
    11701 cggtacgtca tggcattgta ctggccggta tcctcactcc cgcagaacgt caccctggcg
    11761 ggcaatgcag gtaacaacct gctggcgtcg ttctacatcc agacagacgc aaaagacatg
    11821 aacgtgatgt atcacaatgc aaaagccgcg aaggataacc agaaactggg aacatttggc
    11881 gcattcgata atgaatggca cacactggca ttccgcttcg cagggaataa cagccttcag
    11941 gtaacaccgg ttattgatgg taaggatggg gcaccattca cactgacaca gtcacccgtc
    12001 ggaatgttta ctgcagataa actccacgta acagacgtca ctaaaaatgc cacttacccc
    12061 gtgctgattg acagcattgc cgtggaaatc aataacccgg taaccgcgta aaaaaaatcc
    12121 cgccgctcat tacagctggc gggaggtagt aatacccaac ccattaaaaa tttgtaagga
    12181 gtacccacaa aggagtaaat gtcagttgtc ttactgtttt acccgataac gatttatacg
    12241 gtcaatatca aagacagggt tatatggatc ccgtttacac aatactgctc tgcttttgca
    12301 ccatcacctg cgtgctgttc cttgccagtg gtggcggaga aatcctttat caatggtgcg
    12361 caaaaaaaac ttcatgaatg gctgaacaag tctgaacgtg acacgccaga ccagtgatct
    12421 ttaccggaga ggtgacacat gctcaagatg aacgcattgc gcgataccct ggctgacaca
    12481 tgccgctggt gtaaagccaa tccggataaa ttcactgtct gcgtggaatc cggcagcatt
    12541 gaaacgaccg gtgaatcacc ctcgtttctg taccgttaca acctggtgat ctttgccatg
    12601 gacttcactc aggatatcga cgaactgttc ctccctctgc tgcactggct ttaccacaac
    12661 cagccggatt tactgctgaa tccagaaaaa aacagcagca tcaaattttc agcatccccg
    12721 aataacgacg acagtgttga cgtggttatt gagctgccgg tatgggaacg cgtgagggtg
    12781 acacgcacac ctgacaggca agtccgcgca gaacatctgc aggagccgcg gccccgcatc
    12841 ccctcagcac ggggagcgtg gagcaatgta tacgaagacg taacatggag tataccccgt
    12901 gatgaatgag cacaaacttg atgctgtttt tcgtgacatt ctggccggta tgtccgccgc
    12961 aggtctgtcc cgcacggcca gacaggtggg gcaggcagta cgccgcagcc agcagcagag
    13021 aatgcgtgca caaaaagccc cggatgacac cccctgggca ccacggaaac gtcagattca
    13081 gcgggtacaa cagggtatta agtttctctg gcaacctccg gcagcgggca gccagcccat
    13141 ggtcagggaa ctgaaaaact ggaagcatgg tacaggtcgc cacggcccac acataaccgg
    13201 ttatgacatt aaccgcgggg gactcagaac attctaccgg gctgacattg aagaatacct
    13261 ggaaatcaga acacgccgtg tcagccaggg aaacaccgtc agggcacgga tgtttgaacg
    13321 cctgcgacgg tacgtaaaaa tacttcaggc cgacaatgag agcatccggg tgggttttaa
    13381 cggtgatcag gcccgtattg cgcgtgtaca ccatttcggg ttacgtgaca acgttgtccc
    13441 tggtgtaaca acacaatatc cggcccgtga actgctgggg ttgtcagtaa aagataaagc
    13501 aatgatccgg gaaaccatca tcggaagtct gaaaggaggg atgaagtgag cgcggaaatt
    13561 atgcgcctgc tggaaaatat gatccgggtg ggtgtgattt cagacgttga tttaaaaaac
    13621 tggcgtgtaa gagtggccag cggcaggctg acaacggact ggctgcgctg gaactgcacc
    13681 cgtgccggtg tatttaaaat ctggcttcct ccttctcgcg gtgaacaggt tttactgctc
    13741 tgccctggcg gtaaccctga aatggcaatg gttgtcggga gcctgtacag cacagacaac
    13801 cctgcccccg gaacacagca ggatgaagtc atcatcactg ccagagatgg tgcaagactc
    13861 cgttataacg caaaggaagg tgcccttgac gcaacaggga taaaaacggc cagagtgacg
    13921 gcagaaaccc gaatcactct ggaaaccccg ctcgttgagt gcacacagca cctgaaaacg
    13981 cgcacatttg aactgacaga tggcggcaca ctgaaaggtg acgtaaccca ctcaggtggg
    14041 tcactgacat caaacggcgt ggtcacacac acccacacac actcaggcgt ccagtccggt
    14101 gggagccaga caggaggccc acagtgacag ccacctacac gggtatgaac ccttaccatc
    14161 cgggtgttgt gcacgataca gagcatctgc actgttctgt acgcgatatt ctgctcacac
    14221 ctcagggaag ccgcattatg cgcagggatt acggcagcct ggtacccgat cttctggatg
    14281 ccccccagaa tgatgtaacc cgccttcagt gcatgagtgc cgcagtgatc gccctgaccc
    14341 gccatgaacc acgcctggca ctgaatacca tcgacgttcg ctggctggat gaaggtgggg
    14401 ctgagctttt actgaccggg ataatcaccg caaccatgca gacggtcaga ctgagcatac
    14461 gcacaggaga ctaaccgatg ccaaccgttg atttgtcaca gttgccgaaa ccggcaatca
    14521 tcaacacacc ggattttgaa gttattctca atgagataaa aacgatgatg atcggctgct
    14581 ttccggaaga acagcaacct gctgtaaccg ctgctctcgc actggaatct gaaccactga
    14641 ccattatcgc cgaggtaatg gctttccggg agatgctact gcgccagcgt atcaatgaag
    14701 gtgttgcagc ctgcatgtta agccatgcct ccggtacaga tcttgatcac ctggcagcca
    14761 acctgaatac gcagcgcctg gtgatcactc cggcaacaga tactgccccg gctgtcatgg
    14821 aaaacgacac cgcactgcgc ttacgtgcgc agtcagccta tgaaggcctt agcgtggcag
    14881 gaccatcggc ctcttatgaa tactttgccc gaagtgccag tggtcaggtt gctgatgcca
    14941 gggcagtcag cccatcccct gcagtagtgg tggtgtcaat tctctcaaca gaaggcgacg
    15001 gcactgcatc gcaggaactg attcagaccg tcagtaacgc cctcaacgat gagcgaatca
    15061 ggcctgtcgc agaccggctg accgtccaga gtgcaaaaat cgtaaaatac cggatcaatg
    15121 cccggctctt tctgtatccg gggccggagt ccgaacctgt aattctggct gccagacagt
    15181 cccttgacga atggctgacc gaacagggcc acataggccg tgatgtggcc cgctctgcga
    15241 tcatgtcagc attgcatgta cagggcgtcc agcgggtgga gctggaggaa ccagcaagca
    15301 atatcgcggt tgacgactcg cagttcgcac gctgcgaatc cattatcgtt gatatgggag
    15361 gtaccaatga gtgattccct gcttcctcct tcagccagtg actttctgaa atataccgga
    15421 tatgccactg accgaatcac ccgtatcccg gttgatttgc gtactctctg ggatccggaa
    15481 gcctgtccgg tggagcttct cccctatctg gcctgggcgc tgtctgtcga gcgctgggat
    15541 aaaaactggc cggaggaaac aaagcgaaaa gttatcaggg aatcctggct catccaccgt
    15601 cacaaaggta ccttcagctc actcaggcgc atcgttgaac ctctgggtta catcatcagc
    15661 atgagcgaat ggtgggaaac cggcgatcca cccggcactt ttcgcctgaa tgtgggcgtg
    15721 tttgaagggg ggctgactga agagatgtat tttgaaatag agcgactaat cgccgatgcc
    15781 cgccccgtca gccgtcatct ggtaggcctg aatatcgtgc aggacatttc cggcaaggta
    15841 tacacaggcg gcatggtgta tgacggcgat attattaccg tttacccgat acaggagagc
    15901 gacgcatgag tgatacggta aagtttaaaa ccattatcac gactgccggt gctgaaaagc
    15961 tggcagcctc agcctctgac ggcggcaaaa aagtcacgcc agccgttatg gctgtaggtg
    16021 atggcggcgg aacactgcca gatccggatg cgaaacaaac agcgctcatc aacgaagtct
    16081 ggcgacacga tctgaacagg gtatttattg acaataccag caaaaatacc gtagtggcag
    16141 aactcatcat tccggcagaa acgggcggtt tctggatgcg tgagctggga ctttatgacg
    16201 atgaaggaac actcatcgcc gttgccaata tggcagaaag ctacaaaccc agaatagctg
    16261 aaggcacagg acgttcacaa atttgccgca tggttatcgc ggtcagtaac attgaaacaa
    16321 tcagtctgac tgttgattca acggtcgtta ccgcaacgaa agaatacgtt gataatctgc
    16381 ttgaagagca tgaaaaatcg cgtaatcacc cgcgcgccag tcaggccgca ccgggattag
    16441 tacaactgag cagtcagaca gatcttgact cagaaataat ggctgccacg ccaaaagcga
    16501 taaaaatcgt catggaaaac gccaatggtc ggcttgcgaa aaaccggaat ggcggagaca
    16561 tccccaacaa ggagctgttc ctgatcaacg tgggcgcacc acgcgtttat gcaaaagacg
    16621 ggtacgtggg tgaagcagga caacgctgga caacagaaca attcgtggaa tggctgaatg
    16681 agcgtggcgc cttcagacca ggcctctgga tattcagagc caatatttca ctttcccggc
    16741 acagcgtgct cactgatacg ggctatggtg atatctgcct tggaggctgc cttatcgaag
    16801 tatcaggcga catcaacggg gggatcatca ggatcacgac aggtctcgaa agcccaaacc
    16861 ctgccgacat gtacctccgc acacagttca ccggggttgt tttaaataaa gggaaaagca
    16921 ccatctggta ccgcacttac acggacaaaa acaaacccac accacaggac atcggcgcac
    16981 tgcctgcaag tgagttatta cccgcaggcg ttcccctgcc gtggccgtca gataccccgc
    17041 ctggcggtta cgccatcatg gcgggacaga cattcgataa agcacgatat cccctgctgg
    17101 caaaagccta tccggacggt cgtatcccgg atatgcgtgg ctggacaatc aagggaaaac
    17161 ctgccagcgg tcgtgcggta ttgtcacagg agcaggacgg catcaaatcg cacacccacg
    17221 gggggagcgt ctcaaatact gaccttggca cgaaaacgac ctcatcattt gactacggaa
    17281 ccaaaacaac ctctacaaac ggacaacatg aacacagcgt ccccctgaga agacaagatg
    17341 gcggaggcat aaactttgac tggctggatg gttcatcgtc cggttctcat attggtaatg
    17401 gatacgttcc tgccgcaggt aaccaccaac ataccgtacc cattggcgca cacacccaca
    17461 cgctcgcact gggttcacat ggtcacggcc tgaccattaa ccccaccggt aatgccgaaa
    17521 acaccgtcaa aaacatcgca tttaactaca tcgtgaggct tgcataatga cctttaaaat
    17581 gagcgaacac ccgcagacaa ttaaaatcta caacctgtca gccgggacaa atgaatttat
    17641 cggcgaaggc gatgcctgga tccctccgca taccggactg cctgcgcact caacggacat
    17701 tgccccgcct gacattcctg aaggacacgt ggctatcttt gatgaggatg aaaacgcctg
    17761 gcacctcatg gaagatcacc ggggtaaaac ggtttaccac gaagagaccg gagaaaagct
    17821 ggttataacc gggccgggtc ccctgccatc atccgtgacg accgagccac ctggtacttc
    17881 agcagatgta taccgtgacg ggcaatgggt gacagattta tcagcagcgc gcgggatcaa
    17941 aaacagcgaa attaacgact ggcgtaacag aatggaaaac gctggttata ttatggaata
    18001 caatggccgc aaatgggatt acggcaaagc cacgcaggat cgccttgagc catctgtcac
    18061 agccgccaga gccggaaagt taccggcaga gttcttctgg acagatgcgg aaaataacga
    18121 tgtaccaatg agtgcagacg aactgatagc gctcagcgaa gcagtcggag aggcaatgtt
    18181 taaaaaagga cttgaaattc acatccggca gcgggaaatg aaaaaagaac tggccggaat
    18241 ggataacgcg ggggaaataa tgaaattcac tccgggctgg aaaataacat gacagaatga
    18301 ggcggcagac ttcacgatgc tctgccgccg gatatcacag aatgccagaa gccattgagg
    18361 aaacccgttc tcccgccgca ctggcagcgt ctttcaggtt attcagcaaa tcccccacag
    18421 atgatgcctg aagacgctcc cgcaaatcct cgtcacaacg gcggaaactg acagaaaacg
    18481 taattttttt cgcccgtcca tagcgatcaa actcttcacg ggtcgtctga atatccgtaa
    18541 tcacatacat accgtaaatc tgtccggctc cgtcaatcaa cggccagggg cgccccgtat
    18601 atgcctgagt gttcagcacc gtaagggaaa cctccccgcc cgtgatttcg ggatacagca
    18661 caccggaaag cgtgatttta tcttctccgg ccccgatata ctgccagcca gcagaacggt
    18721 taatacgctc attgctgaca tggcgccagc cccggctctg ctgtaactgc tgatgtggca
    18781 gtgtactgag ctgaaaaaca aacataccgt aaatcatcat catctctcat accctccatc
    18841 atccattaat ccacatccct gaaactacgc agcccgttac gggactgacc ggacaattcc
    18901 gcttttaccg cttcgccaat catccgggca agagttcgcg gatcctgggt aacgacatta
    18961 tgcagatgaa catggatctc cccactgaac cccacagaag acagtccgcg ttctgagcca
    19021 gatgaaggca cagtgagcgg tggtggactg gcaaccttaa tgttctggtg ctcttcaata
    19081 cgggctcctg aaacaccagt actgcccgcc agtcggggtt ctgtccattg tccgcgaaca
    19141 accagcgccg gaggaagatt acgaaaaata atatcaccag gtcccgtgcg ctttttcgtt
    19201 tccctcacga tgtcccgtgt attaccttcg atacctttca gcctgtcaag aacgccacca
    19261 ttcccgctgt ttgcggaggg ctgcaacacg gctggcattt caggtaagcg taggacaccg
    19321 acactctgtg ttcctcctgc tcctttcggc gaccagttcc acgccttctg aaccatcttt
    19381 ttctgctcag ggtcccattc ccagaccaca ggtgttttac cagccgcatc cagtttctgt
    19441 ttcacgcgcg ctgcctctcc gggaagcacc ccaagcttct ccagcaccca ggcaaggcca
    19501 tctatcagcg ccttcatcgg tgaaaacaca agcgtcagag cactgaccag agaactgcca
    19561 aaacgtgctc cggcagctgt acacttatcc agcgtgtcac gcgttgctgc gacaggactc
    19621 aacagcgatt taaaccacag gaataccttg gatacagcac cggcaaccag atccagcaac
    19681 ggtgcaaaac gggacaccgt ggcaagcaaa ggtgcaagcg cccctctcgc accatcgata
    19741 aaccccagaa caaacgccct gagctgatcc cagtatttat agatcagaag cccggcaccc
    19801 agcagcgccg ccacaaccag accgacaggt cctgtaagca ttcccaccgc agtacccagg
    19861 gaagaaatta cgccccccag accaccaaag agaccagaaa caacagaaat acccgcaggc
    19921 agagctctta tcagactcag accaagacgc agttttacca gggggattaa caccataccg
    19981 acggtaacaa ggagtccgcc gatggcagca gacagcccgg caacaacacc ggcgaccata
    20041 agcagtcccc tgaccagttc aggatttttc tgtacccact cccgcattct ggctgtccac
    20101 tgcgtcgccg tctgtaccag cccgcgcaat gtttctgtta ccgtactgac agcatcagcc
    20161 cgtaacccgg caagcgaacc cgtcagcttc agaacgtcac cggagagatt atcacgcagc
    20221 gtattaccca ttcgctcagc tgcgccttct gtgtttccaa gtacgacaga agtacgggca
    20281 agtgccccca gaaatgcagg gatctgatca actgccaggt cctcaacagg cgtaccaaac
    20341 aacgcaatag ccgcattagc ccgttcagcc ggatcgcgaa tgcgcagtaa tccggctgct
    20401 gttttctgca tggcatcacg ggcggcgacc ccgccactgg caatagccga agacatctgc
    20461 gccgcattaa gccctatgct ttcataagcc tcgacgcttg acttcgacat atcagaacca
    20521 cgaatactga actccttcac agcatccccg gttttatcca gtgcaaattt cccctgacca
    20581 gccatatcag ccagcagact catcgcctca gcaccgctga aacccagatt tctgaaatgc
    20641 gttgaatact catgaataat ttcaggaagt tcaccacgca tctccggccc catgcgttgc
    20701 atggcagaga ccatcagatc gagagcctca tcactgcttc gcgccagccc gtttttcatc
    20761 atgatggcag cagcctgtat cccctgagga acatccacgc ccatcactgt ttgcatatcc
    20821 atcatgcgac gggtgatacg ggagatctct gtatcccccg taactccaag cgcccccagc
    20881 gtactacgaa cagctcccac tgcttcagcc gcccgggcag cgtcaccagc aataccggca
    20941 tgatgtagtt cacgaacaag acgtgtatac ccggatgcct cagcacgccc ctctccggtc
    21001 tgggcagcaa tcactgctcc atagcgctct tcattcatca ccggtgcaac catgcggctg
    21061 cccgcataca tcccgccaag accggcaagc atcattgctg taccagcgcc acgcagtttt
    21121 tcagataccg ccatcgcacg ggtatactca tcccttgccc ggctgacccg cgcaagcgcc
    21181 agacgctgcc gctcgagctc ttcgttatac tgttgagttc gcctgattgc cgcctcaatg
    21241 gccctgtccc caccagacaa cgtgacacca tgtcgcctga gcgcttgccc ggcctcccgt
    21301 agtctgtttc tctcctgcgt tctgacttcg ttcagccgtt caagtcgtgc cgtcagcttg
    21361 ttaatcaatt cctgctgcgc ttcagtgagc ggacgaccgc tctgctgaag acgctgaagc
    21421 ccgttcagtt cctgctgggc gcgcttaatt ttgtctgtta cttcggtgag atgtttgcgg
    21481 gcagatgaaa aggagcggga ctgtctttca agatcccgga gattattttg cgtattgcgg
    21541 agagaggcgg ccagcgcttc tgcactttca cgggcactag tggctggccg ggtcaggtta
    21601 tcgactgcac tgaatgcaac tcgaatatca agtgtattca tttttctgga gatccgtttc
    21661 tgtcagccgc ccgcttacgc caggcgatga catcctccgg ccccatcata aacaggtcag
    21721 cgggcgacca gttaaaaaca actgcaatat cagcgacaac atcctcgata tgttcaaact
    21781 gcaggatggt gatcagccgt ccgtctcctc cccgttcggt tttgtcgaca caccaccgac
    21841 ataaaaattt acggcagcat tcgcaagcgc agaaaaatcc cttgcatcca ttgccttcag
    21901 ttcactctct ttcaggcggg gctctgtcac acgggagaga tatgtcagca gcgcatcaaa
    21961 tttcatgcca tacaaatcca ccagagacag cccccgcagc gaacccgcct ggcgggcaag
    22021 tggcccaatc ttcactgatt taatggtttc agtatcacga acaaccggag taatcaggct
    22081 gacaacatcc ggagacgcat tacttccgat atctttgtca gtgaccagtt ctgccgtttt
    22141 cgtcagtttt gcattttcat tcacgtttta ttctcctggc ggtatggcta ccgccgtctg
    22201 ttctttcagc ccatccccag cgcggaacgg atgcggtcgg gaacaatatt ttccccgtcc
    22261 tgtcggtaga tgaagttcag caaatcgaac tccagcagtt cgcgatcatc tgaactcagt
    22321 ttgtaatacg tgtttttcac attgtaggta tgctgggtat cttctccctg ttttgcttcc
    22381 cccatatcga tttcactgat gcgtccgcgc atttcaacct caatcaactg actgtcgcca
    22441 tcggtgtaat actctccggc aaaacgaaga cggacctcat caatacgtcc ggcatactta
    22501 cgcaccaggt cagcggctgc accacccata acaaaactcg catccagtgc gctgtcatcc
    22561 agtccgagat caacagccac acttccaagc atacctcccc cacggtagct ctccgttttg
    22621 cgagtcagtt tgggtaacgt gacagacacc acctgaccaa tttcgttttt accgtctatg
    22681 aaaagcgtca taaaacgcag cttatgcgga accgccatta cgcacctccc agagaagcaa
    22741 atgcaggacc aaagaattca tccgtaaagg tctgatgcag agtcagatct tcaagtggtg
    22801 gcacaggcgt gtatttataa cgaatacgca cccttccctg acgcaaatcc gttgtgctgt
    22861 tatcaatcac gtcataccag cattccgccc caatcagttt tccggcagtt accagttcat
    22921 taagttttgc ccgaatggcg ctgatggcgt ccttcacatt tgcaggcgtg agcggatcat
    22981 caacaacctc aaactgcgct tcggcgatgg tatccgccag agtctgtgcg gttcgtgtat
    23041 agacttcaaa aatgtaggcg ctggtatccg tggtacggtt tccccagaag cgaaaaccat
    23101 tacgtctgat aattgtggtg atttctttgt tattgaggtt attggcatca ctgtcttctg
    23161 actggagaga ccagtagacc ggatgagaaa cacccagcac attttttacc ggttcattag
    23221 acagcgactt gtgccagccc tgttcatgat caagactggc ccgaagaccg caggcataag
    23281 cagcagacgg gaagctttca ttctttcctg actgtgggtt ataggcgata aaatccggcc
    23341 agatcagcat cagttcccga taagcaagat cctgacgcca ggtcatggct tctgtggcag
    23401 tattgcagcc gggacaccct gcataaacaa acgcccgcaa tttaccggca atcgtgcaca
    23461 acgcagtggt aacggctttt gtgtcaagcc caggcgccgc cagaatacgc ggacgatacc
    23521 cgatagcctc atcctgctct gctaccagaa gcgcctgcat accggtatag cgaccatccg
    23581 cttcaaccgc accaataaca agctggtcct gagtcttctg tttgtcacct ttcttttctg
    23641 ccgccacacg aacgacaatc acctttgtgc tgacctgatc ggatatcgcc ttcagggctt
    23701 tatacagcgt acctgccttg cctgccttgc ccagcacatc attcacacgg gtcagcagaa
    23761 caggcttatc cagaggaaac gtgccggtat cggcatcatc tgccacagca acaataccga
    23821 tcacacttga gtcgatatcg ttaattgccg tgacgagatc ggtgctttcc cggacacgcg
    23881 ccccgtgaaa acgtgtttcg ctcatacatt tcaccatcat cttcagaatg aattcacgat
    23941 gatgattacg cagatacacg gggagttcac gatatacagg ttgtgaatca tcacatacaa
    24001 cctgccgggg ttccggccag acggcggtta tgagattttc gccacagata acgggagaac
    24061 acaacaatgg ctgatcggat cattcgcatc ataaaaaact atgatgacag gacgagtacc
    24121 ctggtaaagc ttcctgattt cagcatcacg ctgaacaaca acgaactgga agcacaggaa
    24181 ttaaaaaaca gggtaatgtc gctgtctgta actgacaaca ggggatttga agctgatcag
    24241 atatccctga cactggatga tacagacgga caattaatcc tgccggaacg gggagccgta
    24301 ctttccatcg ccattggctg gaaaggcgaa agcctgacag ataaagggac atatattgtg
    24361 gatgaagttt cgcatgacgg gccaccggac aagcttatca tcggagccag tagtgctgac
    24421 ttccgggaag aattcaatgt caaacgggaa gtatcctggc atgacgtaac gcttgagcgt
    24481 gtggtttccg ccattgcaca ccgatacggt ctgaaagcac aaatcagcga aatgctgatg
    24541 catatcgaaa tcgaccatgc agaccagaca gaagagagtg acatgtcatt ccttacccgc
    24601 atggccgata tgcttggcgc catcgcaacg gtcaaaaacg gcaacctgct gttcatcata
    24661 cctggtggcg gcgtgacagc cagtggcaag ccactcccct ctttcacctt aacccgcagc
    24721 gacggtgatc aacatcgctt ccgtatcgct gaccggcagg catacacagg ggtacgggca
    24781 tactggctgg atctgaattt cgggaagaaa aagcaggtca gtgtaaaaaa acgcagtctg
    24841 tcacaggaaa aaagccacag ccgtgaaggt gattacatgg aaggcgcaga aggcaatgtg
    24901 tttgtgttgc gaagaacctt tccgaatgaa gaaagcgcaa aacgtgcagc agctgcaaag
    24961 tggcaacaat tacagaaagg cgcggctgaa tttgccatca cactggctca cggacgcgca
    25021 gatctctacc ctgaaatgca cggcaccgta tcaggattca aaaccacgat cgaccggcag
    25081 gactggatca ttgccagggt ggaacacact atcgacagca acggtttcac cacacaactg
    25141 gagcttgagg ctaaaatacc tgactggata gcagaatcag aacagagtgg ataaaataaa
    25201 ccacgagttc aaccccgtaa tgactggggg tatcgtcatg ttttattgtc cacactgcgg
    25261 cgcatcggcc cgtatcagaa ccagcaaggc agccaatgat accggcacca tccggtataa
    25321 atactaccag tgcaacaaca tcgaatgtgg tgcgacattc tccacaatgg agagcgtaca
    25381 caaaatgata tccgtaccca ccccctccgg cgaacgggat gaacagatcc cgcacgagca
    25441 tttcccggca acacacagag gtcgccttca gctaatcctc gagctctgac agttgcgacc
    25501 acaaaaggaa atcagtacat tacacgcggg tgcctttcgg ctgacggctg gagggtgaac
    25561 ctgaaggccg gatgtggaaa ggccccgagt caacttaaac gttaacccga ggccctgacc
    25621 atcctaccta gcaagtggaa ggttagcgcc tccctgtaaa aggagtcaag cgctatgccg
    25681 caaaaaacga tcatagtgag cattttatgc ataactattt tgatgattgt ctggatgata
    25741 cacaactcac cctgtgagtt caggcttaac atcatgtggt cacaatttgc ggcgttcttg
    25801 cagtgtaaac agtaagaaac cgcagcgggg gtgaaagccc ccgctatccg gttgccgggt
    25861 tcagtcgata tggccccctt ttctccattg gattacagac gtatttgccc gcaatatgcg
    25921 ggcttttttt gccgccccct caacagcttc aacacagagc attgcgcaca caaagggaaa
    25981 tcagtagatt acacgcgggt gcctttcggc tgacggctgg aagggatacc tgaaggtcgg
    26041 atgtggaaaa gccccggaaa aacacttttg tttaaccgag gccctaaccg cattaccttg
    26101 acaagtgaaa ggttagcgcc tctccactaa ggaggcaagc gccatgtcgc aaaaatcgct
    26161 gacagccatc acgttctgcg tgacggcaat cctcatcatc tggatgctgc acggttcact
    26221 gtgcgaaata cggatgagct tatggggagc ggagtttgcg gcgttcttac agtgtaagca
    26281 gtaggaaacc gcgacgggag agcaatcccc cgtcaatcgg ttgttaaggt taaggctgaa
    26341 aaggcaccct atctgttact tgaaatgaaa agcacagccc gcagcgtaaa aactgcgggc
    26401 tttgtttttc agtactatcg aaaacagccc acacctacaa acagtgaata gtacagagag
    26461 caataagaaa ccagcaataa caggaaagac gattgccact gagtcaactt tactcccgcg
    26521 cctacatgtc gccataccat cgccacttta tcgccacaag ccaaaacaca atcacaaaaa
    26581 aaccacccta aggtggtttc acgacactgc ttattgcttt gattttattc ttatctttcc
    26641 catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc
    26701 cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc gttccggagt
    26761 cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac gcgccaaatt
    26821 cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact ggagcgggaa
    26881 acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt
    26941 cccgcattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc
    27001 cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc
    27061 acttttgatc gtttgctgaa aattgcgccg aaacgatcac tgatcaagca aatctgcacg
    27121 cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag
    27181 tgcaataccg gcgtactcaa cccaaatgtt cggacgccac agcagccagg ccagcgccac
    27241 catctgggca gtggttttca ctttcccaat ccaggagaca gccacgctac tgcgtttacc
    27301 caactccgcc atccattcgc gtaacgcaga aataataatt tcacgggcga tcatcgttgc
    27361 tgccggtaag gtcacccacc agctgtgata atgctcggtt accagcacca tggcgatagc
    27421 cacgagaact ttatctgcta cagggtcaag gaaagcacca aaccgggtac tctggttcca
    27481 gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc
    27541 ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag
    27601 gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta
    27661 tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag
    27721 aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg
    27781 gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg acgttttggc
    27841 ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg
    27901 tgcccgccaa tcgcgtgatc gtgtgattca tcgcgaatat gctggataac atgcagtgcg
    27961 ggagaatctg gcggcaaact gaatccctca ccttccggct caaagaacag cgtttccagc
    28021 ccagccttac gatctgctcc tttggcaacg cctagtagca gcggatgatt tttatcccat
    28081 gagacatcca gttcggcgaa aacatttttc gcctgcgcaa gttggccttt gccgccgtcg
    28141 ataaggatca catccgggat cttactctcg tcgatggctt taccataacg ccgacgcagc
    28201 acctgattca tcgccgcata atcatcgccc ggcgtgatgc cagtaatgtt atagcgtcga
    28261 tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttcc
    28321 cccatggtat ggctgatgtc aaagcattcc atccgcttca cttccggcaa tttcaacacg
    28381 ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt gctggttaag
    28441 gccgtcgccg cattggtgcg cgcgagtttc agataacgcg cccgatcgcc gcgaggtttg
    28501 gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta
    28561 tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt
    28621 aaatagaact gacctacgaa ggtttccacc acctcgctca gttccgtacc gccaggcact
    28681 ttcgggaaat agctgcggct gccgagcact ttaccctgac gaatgaacaa tacgtggaca
    28741 caagccatgc ccgcatcgaa cgccacacca ataacgtcga ggtcgtcgcc ggtattggac
    28801 acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acgtgccgct
    28861 tcttcaaact ccagattctg gctggcagtt tccatacgac taatgagttg cgtaagcacc
    28921 tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct
    28981 tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctgata ttgcagacac
    29041 ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa tatcttttgc
    29101 agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa atattcacct
    29161 ttagcatgct tcgcaccacg atgcatcgcc agacgcggat gggtatcgcc actcaggaag
    29221 ataaagggat aagatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg
    29281 atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gagtaaccgt tacatcaatt
    29341 tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct acggaaatag
    29401 ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca
    29461 tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc ttttgcgtca
    29521 aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca
    29581 ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc
    29641 tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct
    29701 tggtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg
    29761 ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatgtcagaa gcaatgtaac
    29821 gctgccctga atagacagaa cggatcgcac tcacaacttc ctgcggagcc gcgcctttgc
    29881 tgaggtagcc cgcagcaccg gcctgcatga ctttcgctgg taaagggttt tctgtatgga
    29941 cggtaagcat gatgatttta acatcagctg tggaacgcgc gattttacgc gtcgcctcaa
    30001 ggccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgcccggc
    30061 accatttaac ggcgtcttca ccgcacgatg cctctccgac gacttttata ccctttatat
    30121 cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt
    30181 tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt cattcgtata
    30241 ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc
    30301 gtttttgtaa attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttccggga
    30361 tcgtttcact gtaagccctg cgatgaaatg tgacaaaaat tgactttatt cagcaaaaat
    30421 gaaaatcagc c
//
LOCUS       DAZKJR010000052        29916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKJR010000052 DAZKJR010000000
VERSION     DAZKJR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.052"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..379)
                     /gene="emrE"
                     /locus_tag="ACHOWS_002340"
     CDS             complement(47..379)
                     /gene="emrE"
                     /locus_tag="ACHOWS_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFQ0047902.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(447..749)
                     /locus_tag="ACHOWS_002341"
     CDS             complement(447..749)
                     /locus_tag="ACHOWS_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFQ0047903.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(746..1447)
                     /locus_tag="ACHOWS_002342"
     CDS             complement(746..1447)
                     /locus_tag="ACHOWS_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFQ0047904.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1444..2463)
                     /locus_tag="ACHOWS_002343"
     CDS             complement(1444..2463)
                     /locus_tag="ACHOWS_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0047905.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2460..2999)
                     /locus_tag="ACHOWS_002344"
     CDS             complement(2460..2999)
                     /locus_tag="ACHOWS_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFQ0047906.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(3069..3299)
                     /locus_tag="ACHOWS_002345"
     CDS             complement(3069..3299)
                     /locus_tag="ACHOWS_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0047907.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3404..4093
                     /locus_tag="ACHOWS_002346"
     CDS             3404..4093
                     /locus_tag="ACHOWS_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFQ0047908.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4689..4895
                     /locus_tag="ACHOWS_002347"
     CDS             4689..4895
                     /locus_tag="ACHOWS_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFQ0047909.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            4971..5267
                     /locus_tag="ACHOWS_002348"
     CDS             4971..5267
                     /locus_tag="ACHOWS_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFQ0047910.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            5273..6058
                     /gene="bet"
                     /locus_tag="ACHOWS_002349"
     CDS             5273..6058
                     /gene="bet"
                     /locus_tag="ACHOWS_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFQ0047911.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIHASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            6055..6735
                     /locus_tag="ACHOWS_002350"
     CDS             6055..6735
                     /locus_tag="ACHOWS_002350"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFQ0047912.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            6732..6914
                     /locus_tag="ACHOWS_002351"
     CDS             6732..6914
                     /locus_tag="ACHOWS_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFQ0047913.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            6887..7078
                     /locus_tag="ACHOWS_002352"
     CDS             6887..7078
                     /locus_tag="ACHOWS_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFQ0047914.1"
                     /translation="MHKASPVELRTSIEVAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            7155..7370
                     /locus_tag="ACHOWS_002353"
     CDS             7155..7370
                     /locus_tag="ACHOWS_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0047915.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            7469..7678
                     /locus_tag="ACHOWS_002354"
     CDS             7469..7678
                     /locus_tag="ACHOWS_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFQ0047916.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQ"
     gene            7735..8013
                     /locus_tag="ACHOWS_002355"
     CDS             7735..8013
                     /locus_tag="ACHOWS_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFQ0047917.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            7985..8050
                     /locus_tag="ACHOWS_002356"
                     /pseudo
     CDS             7985..8050
                     /locus_tag="ACHOWS_002356"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8212..9375
                     /gene="intD"
                     /locus_tag="ACHOWS_002357"
     CDS             8212..9375
                     /gene="intD"
                     /locus_tag="ACHOWS_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0047918.1"
                     /translation="MSLFRRNEIWYASYSLPGEKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(9391..9467)
                     /locus_tag="ACHOWS_002358"
     tRNA            complement(9391..9467)
                     /locus_tag="ACHOWS_002358"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9431..9433),aa:Arg,seq:tct)
     gene            9738..10604
                     /gene="folD"
                     /locus_tag="ACHOWS_002359"
     CDS             9738..10604
                     /gene="folD"
                     /locus_tag="ACHOWS_002359"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFQ0047919.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            10606..10818
                     /gene="ybcJ"
                     /locus_tag="ACHOWS_002360"
     CDS             10606..10818
                     /gene="ybcJ"
                     /locus_tag="ACHOWS_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFQ0047920.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            10926..11447
                     /gene="ybcI"
                     /locus_tag="ACHOWS_002361"
     CDS             10926..11447
                     /gene="ybcI"
                     /locus_tag="ACHOWS_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0047921.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHKVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(11483..12868)
                     /gene="cysS"
                     /locus_tag="ACHOWS_002362"
     CDS             complement(11483..12868)
                     /gene="cysS"
                     /locus_tag="ACHOWS_002362"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0047922.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            13042..13536
                     /gene="ppiB"
                     /locus_tag="ACHOWS_002363"
     CDS             13042..13536
                     /gene="ppiB"
                     /locus_tag="ACHOWS_002363"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFQ0047923.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            13539..14261
                     /gene="lpxH"
                     /locus_tag="ACHOWS_002364"
     CDS             13539..14261
                     /gene="lpxH"
                     /locus_tag="ACHOWS_002364"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFQ0047924.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            14379..14888
                     /gene="purE"
                     /locus_tag="ACHOWS_002365"
     CDS             14379..14888
                     /gene="purE"
                     /locus_tag="ACHOWS_002365"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0047925.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            14885..15952
                     /gene="purK"
                     /locus_tag="ACHOWS_002366"
     CDS             14885..15952
                     /gene="purK"
                     /locus_tag="ACHOWS_002366"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0047926.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPVQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(16285..17178)
                     /gene="ybcF"
                     /locus_tag="ACHOWS_002367"
     CDS             complement(16285..17178)
                     /gene="ybcF"
                     /locus_tag="ACHOWS_002367"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0047927.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTKDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(17175..17990)
                     /gene="ylbF"
                     /locus_tag="ACHOWS_002368"
     CDS             complement(17175..17990)
                     /gene="ylbF"
                     /locus_tag="ACHOWS_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFQ0047928.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVMAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFIHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKS"
     gene            complement(18001..19260)
                     /gene="ylbE"
                     /locus_tag="ACHOWS_002369"
     CDS             complement(18001..19260)
                     /gene="ylbE"
                     /locus_tag="ACHOWS_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFQ0047929.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPHIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(19270..20937)
                     /gene="fdrA"
                     /locus_tag="ACHOWS_002370"
     CDS             complement(19270..20937)
                     /gene="fdrA"
                     /locus_tag="ACHOWS_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFQ0047930.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSASSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQPHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLVRLLERLQ"
     gene            21254..22303
                     /gene="allD"
                     /locus_tag="ACHOWS_002371"
     CDS             21254..22303
                     /gene="allD"
                     /locus_tag="ACHOWS_002371"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFQ0047931.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            22325..23560
                     /gene="allC"
                     /locus_tag="ACHOWS_002372"
     CDS             22325..23560
                     /gene="allC"
                     /locus_tag="ACHOWS_002372"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFQ0047932.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKQNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            23571..24356
                     /gene="allE"
                     /locus_tag="ACHOWS_002373"
     CDS             23571..24356
                     /gene="allE"
                     /locus_tag="ACHOWS_002373"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFQ0047933.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELEHIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(24485..25630)
                     /gene="glxK"
                     /locus_tag="ACHOWS_002374"
     CDS             complement(24485..25630)
                     /gene="glxK"
                     /locus_tag="ACHOWS_002374"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFQ0047934.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDAYGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(25652..26953)
                     /gene="ybbY"
                     /locus_tag="ACHOWS_002375"
     CDS             complement(25652..26953)
                     /gene="ybbY"
                     /locus_tag="ACHOWS_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFQ0047935.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTSDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(27010..28371)
                     /gene="allB"
                     /locus_tag="ACHOWS_002376"
     CDS             complement(27010..28371)
                     /gene="allB"
                     /locus_tag="ACHOWS_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFQ0047936.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(28432..29886)
                     /gene="ybbW"
                     /locus_tag="ACHOWS_002377"
     CDS             complement(28432..29886)
                     /gene="ybbW"
                     /locus_tag="ACHOWS_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFQ0047937.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLVICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFMVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
BASE COUNT         7525 a         7924 c         7475 g         6992 t
ORIGIN      
        1 taaatataag agcctccata ttttagtcgt ttagaaacaa attattttaa tgtggtgtgc
       61 ttcgtgacaa taaattaata accaacacac cggcacaaat caacatcatg cctataatgg
      121 ctggcaggtc cagccgttgg ccgaaaaatc cccatgacag taagctaatc aggacaatac
      181 cgactcctga ccagatagca taagcaatcc ctgtaggaat ataagccagc gtctgagcta
      241 ataaccagaa tgatgcacaa taacaaataa ttgtaccaac agatggccat aaccgtgtaa
      301 aaccttctga aaacttcatt aaggttgtac caatgacctc tgcaagtatt gcaccaccaa
      361 gataaatata agggttcata gcatattctt tcctgttcaa actggagaga attgtactac
      421 agtttgaact caactcacct gtttcatcat tgtgttccca ttgatgttct tttatatacc
      481 ctcagtaccc gtttcatcgc ggcactctgg cgacactcct taaaaatcag attcgtgctc
      541 acctttcctt cccgttcttc tctggtagcg aaccggtaat acaccgttcg ccagacctta
      601 ccatcaacga ccaggattcc tgcccgcgcc attttagccg cagcctgatt tatgctggtt
      661 acggttgcgc ctgttaccgc ggcaacgtcc tgtgcacaga agctcttatg cgtccccagg
      721 taatgaataa ttgcctcttt gcccgtcata cacttgctcc tttcagtccg aacttagctt
      781 tgatttctgc gatcttcgcc agagcctgtg cacgatttag aggtctaccg cccatgacag
      841 gaagttgttt tactggttca ggtatcgcct caccacggtt aattcgcgcg gtcatacagg
      901 acagttcatc ggcagccttg cgccgtaatt ccgcgtcagt caacgcattg gcccgcatgt
      961 tctgatacag gttggtaacc agccagtagt gcgcgtttga tttccacgga taagactctg
     1021 catccggata cagaccacgc ttccggcaat actcgtaaac catatcaacc agctcgctga
     1081 cgtttggcag cccggcggta acggatgctt cttcccggca ccatgcaaca aactgcccgg
     1141 gtgatggcag aaatggtcta ttctgccgac gggcaacacg cattccggcg ctcacctgtt
     1201 ccatcgtggt gatcccgttt tcccggaaag ccagaaccca ctggcggcgg atttcgttca
     1261 gttcgttctg gtcacgatta gccaggctcg ccgggaaagt tgccagtaac tggctgaata
     1321 caccgttgat tatctgcgct acctgctgta cctgcggctt ttcgtcgtac tgttccggca
     1381 tgttgttggc gatccgacgc atctgctcac ggtcaaagtt aatcatctgt gcggcgatgt
     1441 ttttcataga tccaccccgt aaatccagtc tgtgtttgtc aggtcgagtt ttggtttgct
     1501 agctgtcacg cctgcctgtt gcttgttacg gttgatttcg agttgggtcc acttgtcgcg
     1561 gagtttggcc ggacttagca cgttaccgga ccagaagttg tcctggcatg cccagcggaa
     1621 cagcacgcac atgtcgcggt ggttacgtcc gtcacgttca cgcatcaggc ggatatcgtt
     1681 agcccaccct gcaaaattcg gttttctggc tgatggcgcg atggtcttca ccatgtcaaa
     1741 catccactct gcggcggtca ggtcttctgc tgtcccccac ttgctgccgc tctgaattgc
     1801 agcatccggt ttcaccacag gaagatcgtt ttctggttgg tcagaggatt cgccagaatt
     1861 ctcggacgaa aaaggtttta tattgtcttt tgttagtttg tcttttgtgt ttacctgatt
     1921 cgggtaaacg cctttacctg atttgggtaa acttttttta cctgattcag gtaaatttac
     1981 ctctttcagg taaactttat ttttcttacc tgattcgggt aatgttgacc attcactgac
     2041 cacattatta atgccggtat tccgcccgct ctgaataaaa atcccacgct ttaccagaac
     2101 actttttgca gcagaacact tgtgcggcaa tatcccggtt aattcggaaa gttgctcgtt
     2161 gctaacccaa tccagttttt tattaaagcc atatgttttg cgcatgacag ccagaaagac
     2221 cagaagctgg tgctgtgtta atccggccag catcacagct tccagcaact catttgcaat
     2281 gcgcgtataa ccatcatcga gatctgccac gcgcggctcc ttttgtgccg catccggcac
     2341 tggaaaattg aatatctcag cagtgtttgc cataattcct cccgcaatga gtgtgttacg
     2401 atttgcacct gaaagtcggt tctgttccag cagaccggct ttcgccattt ctgaacctgt
     2461 catatcgccc ccagcatggt agtaaccatc gccatcaatg gaccagccag atctgggtcc
     2521 acacgaaaca tcgacacaat accttcacta atttccttca gtttctggtg gcgtggtgcg
     2581 ttgagaatga cagcctgttt tgcctcactg agttcctttt ccatttcagc caacctagcc
     2641 atgaagctat cctgctcaac caggtaaccg cgatattcca gcggtagtac cgccagaatt
     2701 gccggggtca gttcacgcac gttatttcgg tatttttcag aatcgaattt gttatcgagg
     2761 aagcggaaca gcttctggcg tgcacggctg acatcatcag ggaaatcgat ggtgccgccg
     2821 ccctgctccc gatactcatt cacaatgagt gtggcaacga catcctgatt atctacagcc
     2881 gaccaggcgc ggacggcatc acggattttt tcgtggcctg gcacctgttt tgtttgagaa
     2941 cgatttatca ccgcagtcgg gctaaatccg ctagtctgtt ggtatgtaag tggttgcata
     3001 attgactcct ttagtttgaa ttgactgtta agttgattgc ttattgttaa agagcgtgaa
     3061 atggaaattt aagctgcgtt cttttcggtg tgtggaaaca acttcggaag atccgggcga
     3121 atctggtatg ccttcactac tccaccagta gccgtaacaa tgctgccgac atgttcaggg
     3181 gatacctttg ctttgttgtg aagccactta tagacggcct gctgtgaaac ttcgcaagca
     3241 gcgcccagtt tcttttgtga accaacgata ttgatcgctg ttttgatagc tgggttcata
     3301 acaacctccg tggttaattt gaatcaagat taaaactatg gttgttttta gtcaacaacc
     3361 attttcgttt gatggaataa aaccttggtt gtacatttgg actatgaaaa caacactctc
     3421 agaaagactt aaagaagcca gattagcgcg aggccttaca caaaaggcgc ttggggattt
     3481 ggtcggggtt agccaggctg ctattcagaa aatcgaaaca gggaaagcta atcaaacaac
     3541 taaaatcgtg gagatcgcga acgctttggg tgtgcgcgca gaatggttat cttctggcgt
     3601 tggaaatatg tcagacagta cagtgcaacc aatacaatca actgtcagcc attccaaata
     3661 cttcaagatt gacgttcttg atatagaagt cagtgctggg ccgggagtca tcaaccgtga
     3721 gtttgtagaa gttctacgct cggttgagta ctcgtttgac gatgctcgtc acatgttcga
     3781 tggtaggaag gcggaaaata tccgcatcat taacgtgcgt ggtgacagca tgtcaggaac
     3841 gatcgaacca ggtgatctgc tgttcgttga tatcacagtt aaatctttcg acggtgatgg
     3901 tatctatgcg tttctgtacg acgacacagc ccatgtaaag cgcctgcaaa tgatgaagga
     3961 taagctgctg gtcatctctg ataacaaaag ctactcaccg tgggacccga tcgagaaaga
     4021 cgagatgaac cgggtgttca tcttcggtaa ggttattggg agcatgccgc agacatatag
     4081 gaagcatggg tagtaccaat taaaaattat caactgggca ttgtgctcat tcagtaaaga
     4141 actaattcct atctttgctc taggtagtaa tattaagcca ccgcaataat atctttacct
     4201 aacggcgtaa gaatcccggt caccgtgccg ggttttcttt tgccctcccc tcatcacaca
     4261 caccgttaaa aaaaccacca taacctcgct tcagttatcg ctatgcgatt caagtcacaa
     4321 aataaatcca tcctaaatac aaccagttat atctaaaaca accaataaaa caacttttgt
     4381 tgttgacgat aaaacaacta tagttttaaa taagttcatc gcaacaacac aacgatacgg
     4441 caactacctg attcaccgtt gcgatgaccg cttagatccg cagtttgaat ttcagcaggc
     4501 ttcggggagt gcgaggggtg aaacggacgc gtgaacgtcg gtgtgaccag ctgaaattaa
     4561 ctcaacattt cataccttag tcgcttcaac gaggcggctt agttatgaca accggcggcc
     4621 atccaccgcc tgaatacgcg cagaagtctc tatatgttca gcagcccagc ttacgggcag
     4681 gagtttttat ggttcatcaa cattatggaa cgcagaccgt taatcgaggt gcggtcatgc
     4741 caggaatgct ggtcaaacac aaagatggta cctggactgc atcagctaat ttacgcggac
     4801 ggctttatct gcatcgcggc atcgagcgca cttatacccg tgatttgctc gtggaagttt
     4861 ttctcgacgg acgcggtaac ggcctgaatc actaatcccc tttcctgttt tcctaatcag
     4921 cctggcattt cgcgggcgat attttcacag ccattttcag gaggtcagcc atgaacgctt
     4981 attacattca ggatcgtctt gaggctcaga gctgggcgcg tcactaccag cagatcgccc
     5041 gtgaagagaa agaggcagaa ctcgcagacg acatggaaaa aggcctgccc cagcacctgt
     5101 ttgaatcgct atgcatcgat catttgcaac gccacggggc cagcaaaaaa gccattaccc
     5161 gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc agaacacatc cagtacatgg
     5221 ttgaaaccat tgctcaccac caggttgata ttgattcaga ggtataaaac ggatgagtac
     5281 agcactcgca acgctggcag ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc
     5341 acaggaactg atcaccactc ttcgccagac ggcatttaaa ggtgatgcca gcgatgcgca
     5401 gttcatcgca ttgctgatcg tcgccaacca gtacggcctt aatccgtgga cgaaagaaat
     5461 ttacgccttc cctgataagc agaacggcat tgttccggtg gtgggcgttg atggctggtc
     5521 ccgcatcatc aatgaaaacc agcagtttga tggcatggac tttgagcagg acaatgaatc
     5581 ctgtacatgc cggatttacc gcaaggaccg taatcatccg atctgcgtta ccgaatggat
     5641 ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca cggggccgtg
     5701 gcagtcgcat cccaaacgga tgttacggca taaagccatg attcagtgtg cccgtctggc
     5761 cttcggattt gctggtatct atgacaagga tgaagccgag cgcattgtcg aaaatactgc
     5821 atacactgca gaacgtcagc cagaacgcga catcactccg gttaacgatg aaaccatgca
     5881 ggagattaac actctgctga tcgccctgga taaaacatgg gatgacgact tattgccgct
     5941 ctgttcccag atatttcgcc gcgacattca cgcatcgtca gaactgacac aggccgaagc
     6001 agtgaaagct cttggattcc tgaaacagaa agccactgag cagaaggtgg cagcatgata
     6061 ccggacatta tcctgcagcg taccgggatc gacgtgagag ctgtcgaaca gggggatgat
     6121 gcatggcaca aattacggct cggcgtcatc accgcttcag aagttcacaa cgtgatagca
     6181 aagccccgct caggaaagaa gtggcctgac atgaaaatgt cctacttcca caccctgctg
     6241 gctgaggttt gcaccggtgt ggctccggaa gttaatgcta aggcgctggc ctggggaaaa
     6301 cagtacgaga acgacgccag aaccctgttt gaattcactt ccggcgtgaa tattactgaa
     6361 tccccgatca tctatcgcga cgaaagtatg cgcaccgcct gctcccccga tggtttatgc
     6421 agtgacggca acggccttga actgaaatgc ccgtttacct cccgggattt catgaaattc
     6481 cggctcggtg gtttcgaggc aataaaatcg gcttacatgg cccaggtgca gtacagcatg
     6541 tgggtgacgc gaaaagatgc ctggtacttt gccaactatg acccgcgcat gaagcgtgaa
     6601 ggcctgcatt atgtcgtgat tgagcggaat gaaaagtaca tggcgagttt tgacgagatg
     6661 gtgccggagt tcatcgaaaa aatggacgag gcactggctg aaattggttt tgtatttggg
     6721 gagcaatggc gatgacgcat cctcacgata atatccgggt aggcgcgatc actttcgtct
     6781 actccgttac aaagcgaggc tgggtatttc ccggcctttc tgttatcaga aatccactga
     6841 aagcacagcg gctggctgag aagataaata ataaacggga ggcggtatgc acaaagcatc
     6901 tcctgttgag ttaagaacga gtattgaggt ggcacatagc cttgctcaaa ttggagtcag
     6961 gtttgtgcca ataccagtag aaacagacga agaatttcat acgttagcca catccctttc
     7021 acaaaagctg gaaatgatgg tggcgaaagc agaagcagat gagagagacc aggtatgaca
     7081 accactgaat gcatttttct ggcagcgggc ttcatattct gtgtgcttat gcttgccgac
     7141 atggggcttg ttcaatgaca cctcagcaag aaaacgccct tcgcagcatt gcccgtcagg
     7201 ctaattctga aatcaaaaaa gccagacagc attttccgga taaaaacgtc gatgacattt
     7261 gccgtagcgt actaaagaag caccgcgaaa cggtaacgct gatgggattc acaccgactc
     7321 atttaagcct ggcgatcggc atgttgaacg gcgtctttaa ggaacggtaa acatgaaaag
     7381 caaaatcatc agggagctac aggctccttt tttattattc gcatttaccc tcaagcgtat
     7441 taaccaacaa ttcagggatt aatgaaagat ggcagacatc attgattcag catcagaaat
     7501 cgaagaatta cagcgcaata cagcaataaa aatgcgtcgt ctgaactacc agactgtatc
     7561 cgcaactcat tgttgtgagt gtggcgatcc gatagatgag cgaagacgcc tggctgttca
     7621 gggttgtcgg acttgtgcga gttgccagga ggagatcgaa cttaagaaca aacaataggg
     7681 actgtgatgg cctcaaagca gcaaatttca acatcgtcca actgaggtgt aaaaatgttc
     7741 agaatcattt ttcctaacac ctggtacgtc gaccaccacg gcactccctg caaaatcctg
     7801 cgttctaccc acaacaaagt tcactacatc cgaaaaggca gaacatgtat cgccagcatg
     7861 ttccgcttta atcatgactt tgaacctgtg aataaagctg atgcagatcg gatagcagaa
     7921 gagatcgaaa cggcagaaca cattaagaag ttacgtgaca tgcgttcaaa aagcagaggt
     7981 aaccatggaa tcatacagcc tcacactcga tgaggcctgt cagtttctta agatatccag
     8041 accaaccgcc accaactgga tacgaacagg ccgcctacag gcaacacgta aagatccaac
     8101 caagccaaaa tctccttacc tcacaacacg gcaagcctgc attgcggcgc ttcagtctcc
     8161 gctgcatact gtccaggtga gcgcgggtga tggcataaca gaggaaagaa aatgtcactc
     8221 ttccgcagaa atgaaatatg gtatgcctcg tattcgctcc cgggcgagaa acgaattaag
     8281 gaatctcttg gcacaaagga caagcggcaa gctcaggagt tgcacgacaa gcgaaaagca
     8341 gaactctggc gagtagaaaa gctaggggat ttacctgatg tcacttttga agaggcctgc
     8401 ctaagatggc ttgaggaaaa agctgataaa aaatctctcg attcagataa aagccggatt
     8461 gagttctggc ttgaacattt tgagggtata aggcttaaag atatctcgga ggcaaagatt
     8521 tactctgctg taagcagaat gcataacaga aagacgaaag aaatatggaa acagaaagtt
     8581 caggccgcca tcaggaaagg taaagaaccg cctgtttatg aaccaaagcc agtatcaact
     8641 cagacaaagg caaagcatct tgccatgata aaggccattc tccgtgctgc agaacgcgac
     8701 tggaagtggc tggaaaaagc gcctgtcatc aagataccag cggtcagaaa caagcgagtc
     8761 agatggctgg aaaaggagga agcaaaacgc cttattgatg agtgccccga accactgaaa
     8821 tctgtcgtca agtttgcgct ggcaactggt ctgagaaagt cgaacatcat aaatctggaa
     8881 tggcaacaaa tcgacatgca gcgacgagtt gcctgggtga atccagaaga gagcaaatca
     8941 aaccgcgcca ttggtgtggc gctgaacgat accgcctgta aagtgttgcg tgatcaaata
     9001 ggcaagcatc acaaatgggt gtttgtacat accaaggcgg ctaagcgagc agatggaaca
     9061 tcaacgcctg cggtcaggaa gatgcgcatc gacagcaaga catcatggct atcagcttgt
     9121 cgtcgtgcag gaattgaaga tttccgtttc catgacctca gacacacctg ggcaagctgg
     9181 ctgattcagt caggcgtccc attatcagtg cttcaggaaa tgggcggatg ggagtccata
     9241 gaaatggttc gtaggtatgc tcaccttgcg cctaatcatt tgacagagca tgcgaggaaa
     9301 atagacgaca tttttggtga taatgtccca aatatgtccc actctggaat tatggaggat
     9361 ataaagaagg cgtaactgat tgaattgtaa tggcgcgccc tgcaggattc gaacctgcgg
     9421 cccacgactt agaaggtcgt tgctctatcc aactgagcta agggcgcatt gataccacgg
     9481 tgtgctgtaa tcgcgtgaat tatacggtca acccttgctg agtcaatggc ttttgaacca
     9541 gttgctgaac aagtgtacga ccgcatcaga tgtagctcac tatggcggca aaccaacgac
     9601 ggttatcatg ataggtgtga atcagccagg tacagaaggt tttacttttc agaaaacgaa
     9661 aactgacagc gcgccacgct tctgacaaaa tagacgcatc cccttcgacc tacgtaacag
     9721 atggaatcct ctctctgatg gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg
     9781 tgcgctctga agttgctcaa aaagttcagg cgcgtattgc agccggactg cgggcaccag
     9841 gactggccgt tgtgctggtg ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac
     9901 gcaaggcttg tgaagaagtc gggttcgtct cccgctctta tgacctcccg gaaaccacca
     9961 gcgaagcgga gctgctggag cttatcgatg cgctgaatgc cgacaacacc atcgatggca
    10021 ttctggttca actgccgtta ccggcgggta ttgataacgt caaagtgctg gaacgtatac
    10081 acccggacaa agacgtggac ggtttccatc cttataacgt cggtcgcctg tgccagcgcg
    10141 cgccgcgtct gcgtccctgc accccgcgcg gtattgtcac gctgcttgag cgttacaaca
    10201 ttgatacctt cggcctcaac gccgtggtga ttggcgcatc gaatatcgtt ggccgcccga
    10261 tgagcatgga actgctactg gcaggttgca ccaccaccgt gactcaccgt ttcactaaaa
    10321 atctgcgtca ccacgtagaa aatgccgatc tactgatcgt tgccgtcggc aagccgggct
    10381 ttattcctgg tgactggatc aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc
    10441 tggaaaatgg caaagttgtg ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat
    10501 acattacgcc tgttccgggc ggcgttggcc cgatgacggt tgccacgctg attgaaaaca
    10561 cgctacaggc gtgcgttgaa tatcatgatc cacagggtga gtaacatggc gacattttct
    10621 ttaggtaaac atccgcacgt tgagctgtgc gacttgctga aactggaagg ctggagcgaa
    10681 agcggcgcgc aggcgaaaat cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt
    10741 gaaacgcgca aacgctgcaa aatcgtcgcc gggcagacag tgagttttgc aggtcacagc
    10801 gtacaggttg ttgcctgatc cctttcccgg cctgtaacaa caggccggtt ctcttttcag
    10861 acttttgccc tctacgcccc gttcatccat gctgatagca tcgacatttt gccgaagcga
    10921 ccatcatgcc aaccgttatt acgcacgccg ccgttcccct ttgcattggt ttagggttgg
    10981 gttcgaaagt catccctccg cgtctgttat ttgccggaat cgtcctggcg atgctgcccg
    11041 atgccgacgt gttgtcgttt aaatttggcg ttgcttacgg caatgttttt ggtcatcgcg
    11101 ggtttaccca ttcgctggtg tttgcgtttg ttgtcccgct gttatgtgtg ctgattgggc
    11161 gtcgatggtt cagggctggg ctgattcgct gctggctgtt tttaaccgtc tcgttgctat
    11221 cgcacagctt gctggattcg gtaaccactg gcggtaaagg ggttggctgg ctgtggccgt
    11281 ggtcagatga acgctttttc gctccctggc aggtgattaa agtcgcgccg tttgcattgt
    11341 ctcgttacac cacgccgtac gggcataagg tgattatttc tgaattgatg tgggtgtggt
    11401 tgccggggat gctactgatg ggaatgttgt ggtggcgcag acgataaccg gatgcgaaaa
    11461 ctcgcatccg gcaatagcgc aattacttac gacgccaggt ggtcccttgc gggccatctt
    11521 ccagcacgat ccccatctcg ttaagacggt cacgcgccgc atctgccgcc gcccagtctt
    11581 tcgctttacg ggcatccaga cgctgttgaa ttaacgcttc aatctcagcc acttcactgt
    11641 cgtctgcctg cgcgccgctt tgcaggaacg cttccggttc ttgctccagc aggcccaata
    11701 cagcggaaag tttacgcagg tgagacgcca ttgcattcgc cgctgccata tcttctgctt
    11761 tcagacggtt cacttcacgc gccatatcaa acagtacgga ataggcttcc ggggtgttga
    11821 aatcgtcgtt catcgcctca ataaagcgcg cttcaaacgc ttcgccaccg gcaggagcaa
    11881 cggttttgtc tgtgccgcgc aaggcagtat agagacgttc cagcgccgca cgcgcctgct
    11941 tcaggttctc ttcgctgtag ttcaactgac tgcgatagtg acccgacatc aggaagtaac
    12001 gcacagtttc cgcatcgtag tatttcagca catcacgcac ggtgaagaag ttacccagcg
    12061 atttagacat cttctcgcgg tcaaccatca ccatccccga gtgcatccag tagttcacat
    12121 actgaccatc atgggcacag gtggactggg caatttcgtt ctcgtggtgc gggaacatca
    12181 ggtcagaacc gccgccgtgg atatcaaagt ggttacccaa ctgtttgcag ttcattgccg
    12241 aacactcaat gtgccagcca ggacggcccg cgccccacgg agacggccag ctcggttcgc
    12301 cctctttcga catcttccac agaacgaaat ccatcgggtt gcgtttgtcg tcgaccacgt
    12361 caacgcgcgc gcctgcctgc agctgatcga gatcctgacg cgacagcacg ccataagttg
    12421 gatcggtcgg gacgtcgaac atcacgtcgc cgttgtccgc cacataagcg tgacctttgg
    12481 cgatcagttg ttcagtgagt tcaataattt cggcgatatg gtgcgtcgcg cgcggctcca
    12541 tatccgggcg cagaatgttc aaagcatcaa aatctttgtg catttcggcg atcatgcgat
    12601 ccaccagcgc cacaaagctt tcgccatttt cattggcgcg tttgatgatt ttgtcgtcga
    12661 tatcagtaat gttgcgcaca tacttcagct tatagccgag gaaacgcaga tagcgcgcaa
    12721 ccacgtcaaa ggcaacaaag gtacgcccgt gaccgatatg acagagatcg taaacggtga
    12781 ttccacacac gtacatgccg acttccccgg cgtgaatagg cttaaattcc tctttttggc
    12841 gtgtcagagt attgaagatt tttagcatcg aagattccgt ttagacatgt gtgggtaatt
    12901 gagttgcgta taatacccat atttcccgcg cgaatcagca tacattgcgt gatgatccga
    12961 tcgtgcggtt atgctataac accaccctat atatgacccg aaccgggttg aagcaccaat
    13021 caaacggaac aggatgcaaa aatggttact ttccacacca atcacggcga tattgtcatc
    13081 aaaacttttg acgataaagc acctgaaaca gttaaaaact tcctggacta ctgccgcgaa
    13141 ggtttttaca acaacaccat tttccaccgt gtaatcaacg gctttatgat tcagggcggc
    13201 ggttttgaac cgggcatgaa gcaaaaaacc accaaagacc cgatcaaaaa cgaagctaac
    13261 aacggtctga aaaatacccg tggtacactg gcaatggccc gtactcaggc accgcactct
    13321 gcaactgcac agttcttcat caacgtggtt gataacgact tcctgaactt ctctggcgaa
    13381 agcctgcaag gttggggtta ctgcgtgttt gctgaagtgg ttgaaggcat ggacgtggta
    13441 gacaaaatca aaggtgttgc aaccggtcgt agcggtatgc accaggacgt gccaaaagaa
    13501 gacgttatca ttgaaagcgt gaccgttagc gagtaatcgt ggcgacactc tttattgcag
    13561 atcttcatct ctgcgcggaa gaaccggcga tcaccgccgg ttttctgcgt tttttagcgg
    13621 gggaagcccg caaggccgac gcgctgtata ttcttggcga tctgtttgaa gcatggattg
    13681 gcgacgacga tcccaaccca ctccatcgcc agatggcggc agcgatcaaa gcggtgtccg
    13741 attccggcgt tccctgttat ttcattcatg gcaaccgtga ttttctgctc ggcaaacgct
    13801 ttgcccgtga aagcggcatg acgttattgc cggaagaaaa ggtgctcgaa ctttatggtc
    13861 gccgggtgtt gattatgcat ggcgacacgc tgtgcaccga tgacgccggt tatcaggctt
    13921 ttcgcgccaa agtccacaaa ccctggctgc aaacgctatt cctcgccctg ccgttgtttg
    13981 tgcgcaaacg cattgccgcg cgaatgcgcg cgaacagcaa agaagccaac agcagtaaat
    14041 cgctggcgat catggacgtt aaccaaaacg cggtggtcag tgcgatggaa aaacatcagg
    14101 tgcaatggct gattcacggg catacccatc gcccggcagt acatgaactt atcgccaatc
    14161 agcaacctgc tttccgcgtc gtgctgggtg cctggcatac tgaaggttcg atggtgaaag
    14221 tcacggcgga tgacgttgag ttgatccatt ttccatttta aaaaacgcgc aactttgctg
    14281 atttcataac cgcgcaaccg ttttccttgc tctctttccg tgctattctc tgtgccctct
    14341 aaagccgaga gttgtgcacc acaggagttt taagacgcat gtcttcccgc aataatccgg
    14401 cgcgtgtcgc catcgtgatg gggtccaaaa gcgactgggc taccatgcag ttcgccgccg
    14461 aaatcttcga aatcctgaat gtcccacacc acgttgaagt ggtttctgct caccgcaccc
    14521 ccgataaact gttcagcttc gccgaaagtg ccgaagagaa cggttatcag gtgattattg
    14581 cgggcgcagg cggcgcagcg cacctgccag gcatgattgc cgccaaaacg ctggtgccag
    14641 tgctgggcgt gccagtacag agcgccgcac tgagcggtgt cgatagcctc tactccatcg
    14701 tacaaatgcc gcgcggcatt ccggtgggta cgctggcgat tggtaaagct ggcgcggcaa
    14761 acgcggcgtt actggcagca caaattcttg cgactcatga taaagaactg caccagcgtc
    14821 tgaatgactg gcgcaaagcc cagaccgacg aagtgctgga aaacccggac ccgcgaggtg
    14881 cggcatgaaa caggtttgcg tcctcggtaa cgggcagtta ggccgtatgc tgcgtcaggc
    14941 aggcgaaccg ttaggcattg ctgtctggcc ggtcgggctg gacgctgaac cggcggcggt
    15001 gcctgttcaa caaagtgtga ttaccgctga gatcgaacgc tggccggaaa ccgcattaac
    15061 ccgcgagctg gcgcgccatc cggcctttgt gaaccgcgat gtgttcccga ttattgccga
    15121 ccgtctgact cagaagcagc ttttcgataa gctccacctg ccgaccgcgc cgtggcagtt
    15181 acttgccgat cgcagcgagt ggcctgcggt gtttgatcgt ttaggtgaac tggcgattgt
    15241 taagcgtcgc actggtggct atgacggtcg cggtcaatgg cgtttacgcg caaatgaaac
    15301 cgaacagtta ccggcagagt gttacggcga atgtattgtc gagcagggca ttaacttctc
    15361 tggtgaagtg tcgctggttg gcgcgcgcgg ttttgatggc agcaccgtgt tttatccgct
    15421 gacgcataac ctgcatcagg acggtatttt gcgcaccagc gtcgctttcc cgcaggccaa
    15481 tgcgcagcag caagcgcaag ccgaagagat gctgtcggcg attatgcagg agctgggcta
    15541 tgtaggcgtg atggcaatgg agtgttttgt caccccgcaa ggtctgctga tcaacgaact
    15601 ggctccgcgt gtgcataaca gcggtcactg gacacaaaac ggtgccagca tcagccagtt
    15661 tgagctgcat ctgcgggcga ttaccgatct gccgttaccg caaccggtag tgaatagtcc
    15721 gtcggtgatg atcaacctga ttggtagcga tgtgaattat gactggctga aactgccgct
    15781 ggtgcatctg cactggtacg acaaagaagt ccgtccgggg cgtaaagtgg ggcatctgaa
    15841 tttgaccgac agcgacacat cgcgtctgac cgcgacgctg gaagccttaa tcccgctgct
    15901 gccgccagag tatgccagcg gcgtgatgtg ggcgcagagc aagttcagtt aattgatgct
    15961 cattttcttg ccggatgcgg cgtgaacgcc ttatccggcc caccgctcca acaccgactg
    16021 taggcctgat aagacgcgcc agcatcgcat caggcatggt gtgccgattg caggatgcgg
    16081 cgtgaacgcc ttatccggcc taccgttcca acaccgactg taggcctgat aagatgcgcc
    16141 agcatcgcat caggcatggt gtgccgattg ccggatgcgg cgtgaacgcc ttatccggcc
    16201 taccgttccg acaccgcctg tagacctgat aagacgcgcc agcatcgcat caggctagat
    16261 gtgccaattg cctggtgccc atcactacag cgaaatacaa gtccccgctt cgcccgccag
    16321 cgtctcttca atccgcgata acgccccaat ccacgcgggt ttgccacggc ttctgacata
    16381 gccactcacc gcagtcacct tcggccccat cgaaccatcg gctttggcaa atggcgctaa
    16441 ctcatccggt gtggcatggc gaatggcacg ttgctgcggc gttccccagt tttcatatac
    16501 cgcatcagca tcggtgagga tcaccagtcc atcggcatta atctgctcgg cgagcaacgc
    16561 agcggcgaga tctttatcaa ttaccgcttc actccctgcc ccgtctttcg tcacaggcac
    16621 accgccgccg ccactgcaaa tcaccacatg cccctctttg agcaacaact cgatggcttc
    16681 gctgtcgaga attttacgcg gttgcggaga cgccaccacc cggcgtaaat atttaccatc
    16741 acgtttcatc tgccagccat aagccgcttc cagtgcctct tgttcttctg gctgataaac
    16801 cggaccgata aacttctctg gctgcaaaaa cgccggatca tcaggcgaaa cttcaatgcg
    16861 cgtcagcact gtcgtgacag gcggcatctg cggctgtgcg ctcaagctct gcgccagcat
    16921 atagccaatc atcccctggc tttccgccac cagcacatcc agcggatacg gttctacctc
    16981 tttccacgcc agattctgta acgccagcag ccccacctgc ggcccgttgc cgtgaacaat
    17041 cgccagccga taagaacggg ccaggcgcgc cagtgcgggt acagcactgg caatgttgcg
    17101 atattgattt tctgccgtca gcgcctcacc gcgctggagt aaggcgttgc ccccaagagc
    17161 cacaaccagt gttttcatga ttttccttgt aataattgtt ggccaagcca gaaccccagc
    17221 agcgtatctg cccctgacgt atgccccagc gccagcagcg aatcaatcgc aacagcggta
    17281 cgtttcgggc aactcagagc atgaataaag tgcaggagtg gcgaggcgaa gtatccttgc
    17341 gcggcataac gtaaataact gacgctcacc gctgtggtaa cgagttgaag attgtcggaa
    17401 caggcaaaaa acgggcgacc ggagcgcgca tctaaagcgc cataatacca ggccgccagc
    17461 agcattccgc tcagcgtgtc atcatgactt ggcgttaatc ctgggccttt acccagccag
    17521 tgccgccagt cggtcttaac gccattgagc gcggcctgaa aacagtgacg aaactggcgt
    17581 aattcagcag gaagcggatc gcctgccgcc aacgccagtg gcccgaaaag cccggtttcc
    17641 tccgcgcgtt gcatccatgc cgctgcaagc ggttgaggat gcgcaggcgg cgtaatacgc
    17701 agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgtc cgaggcgaat cccttgagcc
    17761 ataacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa ttgcgcgcgg
    17821 cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt taacagttcg
    17881 ccgctttccg tcatcaggtt aatcgcccgc cgccacactc ccgctaaccg ccaggactgg
    17941 cgataattcg gtgcgctact actggcaagc agaggatgga tgatcgtcat tgtgcgttcc
    18001 tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgctcaaagc acgccagcgg
    18061 tgcccgcacg gtgcctgcgc caatctgccc gatccccgcc tctttatggg cgattccggt
    18121 attgatgagc ggcgtaatgc cggtttctac cacgcgacga atatccagcc ccaggcacgc
    18181 gccctggaaa tcccagccgg ggatctgcaa ctgcatattg cgttcaagga agatttccgc
    18241 catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa agcgcgttac
    18301 gccaggcgct gcgatcatcg ccgcaccgcc aataccaaag gtttcggtaa ttgcgctatc
    18361 gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagcc cttggggagt
    18421 gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc cgaacatatt
    18481 gccgttgcgg gtcatagccg tgacgatgct gcctgcgcgg atcatcgcac cagcatccat
    18541 cgccgccttg cagtaagcca tcgcgaggtt aaggaagaac tgatcggtca cgctgaggaa
    18601 atccatcact tccgcgatgt gctgtttatc atgatcgagg cgagcaatgt gtggggccag
    18661 cgcacgcatt aacagtgctg aggaagcaat attgcgttga tggaactcat cgcccatggt
    18721 aatgccctgc gccatcatcg cggtgagatc gataccgcgc tccatgcgcc ccagcgccgc
    18781 gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagaa catcttcgcc
    18841 gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt acgcccggtt
    18901 gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa tgccgcccat
    18961 tggcccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct gatccagcat
    19021 tgccagcgcc tgcgcttcat ctttcgccca gccttcaaac agacatgcgc ccacacatgc
    19081 ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt gaagcagtgt
    19141 tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca gccagtgtgg
    19201 acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg atgtaaacat
    19261 gggaacccct tattgcaaac gttctaataa acgaaccagt tttttattgc caccggcgac
    19321 tggcgaccat tggtaatgca caaccggttt gctggcgctt tgtagctcca gcgcaaagct
    19381 gcgtaatccg atgttaatca cggcgacgtt ttccagtaat gacggtgtat gtggctgtgt
    19441 ggcaggcgag agcggatgaa ttaacgccgc cgccagcaag gtggcttccg gcagcgaact
    19501 cacgaccgca atccccgcat cttccagcgt ggcgatttgc tgcgagcggc attgcgggtc
    19561 acgttcagtg cctgtcaccg tggcaatggc gtacagcggt tggctgtcag aacgcgcggc
    19621 acaggctttt tgccaggcgc tcaccagcga ggcggcagga tcggcggtcg caccgaagcc
    19681 aatcacgaca tcaagcagca acacgcgcac ttgcggttta gcgccgagat cggcaattaa
    19741 caggttgcgt aaggtcggat cgatcatcgg atggggacgc ccgacggtgt aaaaatcatc
    19801 gcccaggtcg aggatctgat gtccgtcggc gtccagcatc atgccatgtt gatgggtatc
    19861 gtcggcttcc acgccaaggt gtccggcaag taatcccgcc gcttcagcag ccagcgtacc
    19921 gccggtatac aagccgcaaa taaatccgct gctgacaggc gctattgcgt tacgtcgcgc
    19981 agtgacgcgt gaaagcagac aagccaggcg tgcggcctca tccagcgagg aggcaaacca
    20041 gacattctcg tcgcgggcca ccgccggggt ataacctaaa aacagcgcca ccgtcggttt
    20101 gccagttgct ttcatggcat taacaatttt cagacgcacc gcttcggcag gtggttttga
    20161 aacaaatgcc agcacttcgc ttttctcgtc tgcactgagc atttccagcg ccgtcagcgc
    20221 actgatgccg cccacttcac ggctgaggtc gcgcccgcca aggccaatcg cgtgagtaat
    20281 tccctcccct gccagcgcaa tctgtgagca cagctcctga atcccggtac cggaagcgcc
    20341 aatgacgcca atgttgcctt ccggtatcac gttagcaaaa gccagcggcg tgccggcaat
    20401 catcgacgta ccgcagtccg gccccatcac cagcaagcct ttttcccgcg cgcgggtttt
    20461 aagttggatt tcatcttcca gcgtgacgtt atcggagaac atcatcacgt tgaggttgcg
    20521 atccagcgcc tggttcgcca gctccgccgc atactcacca gccactgaaa tcagcgccag
    20581 attggcatcg ggtaatttct gacaggcact gtcccagcga cgcacctgcg tcaacgcctg
    20641 gctgctggct gacccctgcg ccagttgttt tagcgcctct tcaagctgct gcataatcgc
    20701 ctgcgcgatc cccgcatccg ccgcttcgct acgaattgcc acgcaaatat cgttcggcgt
    20761 ggcgttatta aaatcgtcat gccagaaacc tgtggtatct aataacgctt tattggcggg
    20821 cgtacccatc attacggaaa catcatcaac attttctgat tcgctgagtt ttcgtgaaat
    20881 aatcattaaa ctgaccgaat cctgaaaaca ccctttttta ataaaggcgt ggatcataac
    20941 aactccttaa aaattgcacc catggcaatt gcacgagcag tgttaatgtg gcgattacgg
    21001 tatcgatgac aacgagtaaa taaggtgatg aatatcacac agggattatt aacaaaagaa
    21061 ttcaaataac cgccatgcta ttacgtgtta ttttttaata agcgtggttg ttttaaaaac
    21121 caggttaaaa gcgagcggat tatcaaataa attctaacta tatttttttg cctgtctgga
    21181 tcacataatc cagatatttt ccctgtatgt taattgcagt catgcttcac accgtcgtta
    21241 aaaaggaaga cagatgaaaa tcagtcggga aacactccac cagctaattg agaataaact
    21301 ctgccaggct gggttaaaac gtgagcacgc tgcaaccgtg gctgaagtat tggtttacgc
    21361 cgatgccaga gggatccact ctcatggcgc ggtgcgcgtg gaatactacg cggaacgcat
    21421 ttcaaaaggc ggcaccaacc gcgaaccgga gtttcgtctt gaggaaaccg ggccgtgctc
    21481 ggcaatttta catgccgaca atgccgccgg acaggtcgcg gcgaaaatgg gtatggaaca
    21541 tgccatcaaa accgcccagc aaaatggcgt tgcggtggtc ggtatcagcc ggatgggtca
    21601 cagcggcgca atctcttatt ttgtgcagca ggcagctcgc gccgggttaa ttggcatttc
    21661 gatgtgccag tccgatccaa tggtggtgcc gtttggcggc gcggaaattt actacggtac
    21721 taacccactg gcctttgccg cgccgggaga aggcgacgag atccttacct ttgatatggc
    21781 gactaccgta caggcatggg gaaaagtgct cgacgcccgc tcgcgtaata tgtctatccc
    21841 ggatacctgg gcggtcgata aaaacggagc accaacaacc gatccatttg cagttaacgc
    21901 cctgctccct gccgccggac cgaaagggta tggcctgatg atgatgattg acgtcctctc
    21961 aggcgtctta ctcggcttac cgttcgggcg acaggttagt tcgatgtatg acgatttaca
    22021 cgccgggcgt aatttggggc aattacatat cgttattaat ccgaactttt tctcctccag
    22081 cgaattattc cgtcaacatc ttagccagac catgcgcgaa ttaaatgcca ttacccccgc
    22141 gcccggtttt aatcaggttt attatcccgg acaggatcag gatattaaac aacgcaaagc
    22201 cgccgtcgaa ggcatcgaaa ttgttgatga tatttaccag tatttaattt ccgacgcgct
    22261 ttataacacg tcatacgaaa cgaaaaatcc ctttgcgcaa taattattga gacaggactt
    22321 tcttatgatt acacatttcc gtcaagccat agaagaaacg ctgccctggc tttcctcttt
    22381 tggcgctgac ccagcgggtg ggatgacccg tttactttat tcgccggaat ggctggaaac
    22441 tcagcagcag tttaaaaaaa gaatggcagc aagcgggctg gaaacacgtt tcgatgaagt
    22501 ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg ttctgagcgg
    22561 ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg gtgcgctggc
    22621 ggcgtggctg gcaattgact ggctgaaaac gcaatacggc gcgccgttac gtacggtcga
    22681 agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct ggggcagtaa
    22741 aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg ccaaaggaaa
    22801 tagttttgtc gatgcgatga aggcttgcgg atttactctg ccgaacgccc cactaactcc
    22861 gcgtcaggat attaaagcct ttgtcgaact gcatatcgaa cagggctgtg tgctggaaag
    22921 taatgggcag tcaattggcg tggtgaatgc aattgtcggg cagcgtcgtt atacggtgac
    22981 gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc gtgatacggt
    23041 ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga tgggcgaccc
    23101 gctggtgctt acctttggca aggtggagcc gcgtccgaat acggtgaatg tggtaccggg
    23161 taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc gcgatttcac
    23221 ccagcagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg gtattgatat
    23281 cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg ccaccctgac
    23341 agaattgtgt gaaagtgaaa aacagaacta ccgggtaatg cacagtggtg ccgggcacga
    23401 cgcgcaaatt ttcgcgcctc gcgtgccgac ctgcatgatt ttcattccca gcatcaacgg
    23461 gatcagccat aacccggcgg aacgcaccaa tattaccgac cttgccgaag gggtcaaaac
    23521 gttggcactc atgctttatc aacttgcctg gcagaaataa ggagttataa atgggatatt
    23581 taaataacgt caccggttac cgcgatgatt tactggctaa ccgtgcgatt gtgaaacacg
    23641 gtaatttcgc actgttaaca ccagacggtc tggtaaaaaa tattattccg ggctttgaaa
    23701 attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt gattatctgg
    23761 tcacactgca tcaaaacggc ggcaaccaac agggcttcgg tggcgaaggc attgaaacgt
    23821 tcctgtatgt gatctctgga aatatcactg ccaaagccga agggaaaaca tttgccttaa
    23881 gcgaaggtgg ctatctttat tgcccgccag gctccttaat gacgtttgtt aacgcccagg
    23941 ccgaagacag ccaaatcttt ttatataagc gtcgctatat tccggtagaa ggtcatgcgc
    24001 cgtggctggt ttctggcaat gccagcgaac tggaacacat tcattatgaa ggcatggacg
    24061 atgttattct gctggatttt ctgcccaaag agttaggttt tgatatgaac atgcatatcc
    24121 tctcttttgc gccaggtgcc agccacggtt atatcgaaac tcacgtccag gaacacggtg
    24181 cctatattct ttccggtcag ggggtttata acctcgacaa taactggatc ccggtgaaaa
    24241 aaggcgatta catctttatg ggcgcttact ccttacaggc cggttatggc gtagggcgtg
    24301 gtgaagcgtt tagttatatt tactcaaaag attgcaaccg cgacgtagag atttaagtca
    24361 taccactcgc ctgattattt ttagctatgt tggattttgc cacggtttat tccgtggctt
    24421 tttttgtagt caattcaggt aaaaaaagat gcgatataaa tcgcatccgc tttaaaggta
    24481 agagtcagtt tttaattccc tgacctattt taatggcgca ggcaatattt cgcgcgctgt
    24541 taaagagatt ggtttcaccg ctggcgagca cttcggctag aggtgccaga cgtggcaaaa
    24601 tgctgaatac cgcgtcaatg ccgtactggt gcaccacttc cacgccatca cccaatacgc
    24661 cagcaatccc aatcaccggg acattaaact gcttcgccac cgacgccacg cccagcggcg
    24721 ctttaccacc tgccgtttgc gagtcgatgc gcccttcccc ggtgatcacc agtgctgcgc
    24781 cctgcactgc ctgcgcaaga ttgaccgcat tcaacacaat ctcaataccc ggtttaatat
    24841 ccgcattgag gaataccgcc gccgcaatgc ccataccgcc cgccgcgcca ccacccgcca
    24901 tctggcagac gttgatttca gtttgctgtt gtaaaacacg ggcgtaattt tgcagcccct
    24961 gctccagctc ttcgaccatt tccggcgttg cccctttttg cgggccgaac accgccgccg
    25021 cgccgcgtgc ccctaccagc ggattatcaa catcacatgc gacttcaata tggcagttcg
    25081 ccagacgcgg atcgcattca tccatctcaa tacttgccac gcgtgctaaa ttgccgccgt
    25141 tagccgccag cacctgaccg taggcatcaa ggaaacgcac accgagcgcc tgcgccatgc
    25201 ccataccgcc gtcaaccgtc gcgctaccac caatgccgag aataatatgg cgaatgccgt
    25261 tatccagcgc atggcgaatt aactcccccg taccaaaact ggaggccagc aacggattac
    25321 gcttttcagg cgcgaccagc atcaggccac ttgctgctgc catctcaatc accgccgttt
    25381 tcccgtcgcc ggtaatgcca taaaaagcat tcactttttc gcccatcggc ccgcagactt
    25441 caagcttcac gatgttaccg tccgtcgcgg cgaccatcgc ctccaccgtc ccttcgccgc
    25501 catccgctat cggcaaacag atatagtgcg catcgggaaa gagggtcgaa aacccggctt
    25561 taattgcctg acaacatttt tctgcactta agctctcttt aaaagagtct ggcgcaatga
    25621 caatcttcat tatcaatcct ttttcaacaa attattcgat gcgttcccac ggaataagat
    25681 tatccataag aacagccagt aaaattccga ccagtaagcc gttactgagc agaggacgaa
    25741 gcgttaatgg aaggtcttgc agatacacag gcggtaatgc cattaaaaat atgccgacaa
    25801 ataacggcaa tgcgagtcga taaatattgc gagcggtaaa cgtgatttgc tggctaaaca
    25861 ccagcgcgga aaagagtaaa ggcagataag aaaccagcat gaccgcgcta ctcactggta
    25921 aagggatact gcaaaacagt cgcgtgagtg ccggaaccag tgccaccagc aggcaaataa
    25981 cgctgccata aataaacgaa cgccgcgtgt aatcgctagt ctgggttaat aaaccaatgg
    26041 atgaaacaaa cggtgaaaat ggaattactg ccagtggcac ggttatcagt gtcataaatc
    26101 cggtcgccac aaagctgcga cgataacgcg tattccccgc gccctgctgc ggataaaaaa
    26161 catccgtgcc ccgaatcgca ccgtaggtat tgctgatatt taccagacct gtaatcaccg
    26221 ccgtcagaat aatccccggc gacaaagcac caccactgcc gagcgggaac cactgccagt
    26281 gcaactcacc ggagagcgaa tgcgaagaag gaaagcaaaa gtaccacaac aaccagccgg
    26341 ttatcgtacc gaccagcagg ccgtaacggg caaaacgttg tggcaggaaa ataatcatcg
    26401 ccagtaccag gcacatcacc gcgaccgaga gcgcgaacgg cggtaattga attttaaaat
    26461 tcgggtcggc tatgccaaac ggcagcccga gcataccttt gaaaaagata gtggtcagtt
    26521 gcgcacccag catcaacata aacaagacca tcaccgacgg cgtaaataac cgtgccaggc
    26581 gatgtcctaa tccgctaaaa ccaatcaaca tcgtcagcac gccggagagc gcaattccca
    26641 ccgccagact ggtagcgata tcgttgatcg gtgtcccgcg cgatgcttcg ccaagggtga
    26701 tagtcaggat ggttccccac cacaggccgc ccggcccctc cataatagcg cgacgatgac
    26761 cgcagaacgc ctgagcgaag caggccagtg cggtagcaag aaaagcatat tgcgtgagcg
    26821 taagcaggct actttgcggc aactgaaaag cagaaagtag cgtaggagga accacaaccg
    26881 tgttgcaaaa aataaagaaa aaccactgaa atcctgataa caggctttcg cggctgactg
    26941 caaaattgaa cataaaaaca ccttaaccgg agaatgcccg ccgcaaggac gggcattgca
    27001 ggggccagat tactgctgat gtttaaggat aaattgacct ttcggcgcaa cagggaagcc
    27061 ctgttcgatg tcgtaaatca catcaccacg taagatggtt ttcgtgatac gcgcgccaat
    27121 ggtacggcca acatacgggc tgactttgtg gcgatattcc agatcgtcat tggtaagaac
    27181 atagctgcta ttcggctgaa tgaagacgaa gtcggcatct tttcctgggg cgatacggcc
    27241 tttttgctgc agaccgaaaa tatctgctgc gttagtcgcc attaatttgc cgaacatcgg
    27301 cagagacata ccgcgtttct gtaccgcttc atcgaacatc acgtccatgc agctttgcag
    27361 accggcgata ccaccccatg ctttcatgat attgccggct ttcatttccg gcggacatgg
    27421 agagtggtcg gaaaccaggc agtcgatttc accgttaaac agtttttccc acatgccttt
    27481 ctggttttcc agatcgcgga tcggcggtga acacttcgcc agagtaccga tttcttcgaa
    27541 ctgatcggta tcaagtacaa agtaatgcgg gcaggattca caagtaacgt cctgaccttc
    27601 ctgacgtgca cgagtcactt cttcaacgcc ttccgggctg ctgatatggc aaacgtgcag
    27661 acggcaaccg gcaactttcg ccagatacag cacgcggcga atcgcttcca cttcggtaaa
    27721 taccggacgc gaagccacat agtcatgagc agttacgcga ccttcacgct tcgcttcttc
    27781 gcccagttcg tcacagatca gcgcgttttc gcagtgcacc agcaccggct gccccagttc
    27841 gcccagcttc tgcgcacctt tgaagaactg ccagtcgttt acatcacgga agtcgttgtc
    27901 gataccgcga tcgccacagg tcgcaacgaa gcatttgaag ccgacaacgc ccacttcatc
    27961 cagctcatgc agacggtcga tgttgtaaga caccaggcca ccgagttgtg ccgcatcgat
    28021 agtcagcttg cctttagcgg catcgaactt cagttcaatt gaagcgcggt caaccgttgc
    28081 tggcagctgg ttgagcggca tttcgatcat ggtggtgata ccaccttttg ccgctgcgcg
    28141 agtaccagtt tcataacctt cccagtggct acgacccggc tcagaaatat gggtgtgcgc
    28201 atcaaccatg cctggagaaa ccaccagacc agacgcatcc atcacttctt ttgcatcgcc
    28261 cagatcctga ccgatagcag caatttttcc gcctttaacg gcgatatcta caacgcgagc
    28321 ttcgttttct aaaataacgg tgccgttttt aatgattaaa tcaaaagaca taacaaactc
    28381 cttgtaaaat acagattggt tttttaactc aatgttcaat atcgggatta attaacctgt
    28441 gactttttgt tctcctgttt tttctgctgc tgtacgtttt tttaataacg cgtaggcagc
    28501 gaatgcgaca ataacgccga caaaccatga aacgcgtgat aaaggctcca tgaacggaat
    28561 aaatttacca ccgagagata aaataacggc gaccaaagtt actgagaacg cggtaaggtt
    28621 aaagccgtta tcgtaatatt tgaaatcacc tgctgcggta tacagttcat caagattaat
    28681 ttgtccgcgc atcaccataa aataatgcgc catcatgaca ccaattaccg gacccagcat
    28741 tccgccgata atatcgagga acagataaat actgtcctga ttttccatta atttccacgg
    28801 gcagatcacc aggctgataa tactggcaat cagtacgccg tttttatagg tcagtttcgt
    28861 cggtgcaata gcggcaatct gataaccggc cggaataata ttaccggtgg cgttagtgga
    28921 aatggtcgtc atcagaataa ccagtaccgc aaagaacgag gcgaacaggc tgtcccaacg
    28981 ctgaacaata tccagcacgt tccaggtgtc agcgccgtaa tgaatactgg ctccggcaat
    29041 aatacatacc ccggcgaccg caaacagaat ataggccaca actaaaccca gcgtttgccc
    29101 cagcgcctgc tcacgaaacg agtgggcgtt ttgcgtaaag tcggatgcgc tcaccgccgg
    29161 tgccgcccag accgcaacca ccgcgtttat caccaccaga aacaggaagc cactgttttc
    29221 tgctttctga ataccgcccg gaatgtagtc aaagattgga ccgatcccga ccagcgaaat
    29281 cgcccaaatc gccataccgc cgaaaacgat atagatgcac gggttaagaa tggcagtgaa
    29341 tttatttaaa acttttccgc cgccaaaacc aataccaacg ttaaccagcc agaagagtaa
    29401 aaaagtaatt aatcccggta gagaaaggcc taacagcgtg aaatcgccac cgagagtcaa
    29461 aaatcccggc cagattttgc caatcagaat caagcaggcc agtgaccccg cgtaacattg
    29521 caggccaaac cacataatgg cagcaatacc gcctcttaat aatccgggaa acagtgcacc
    29581 acgtacgccg taagaagcac gcaggatcat ggcaaacggt acgccgtatt tactgcccgc
    29641 agcaccgttt aataccatta ccgcggcaat gaaaaaggca ctgaggataa ttgccagcat
    29701 aatactaaag gtagacaagc cgagaataaa aaagccgcca accatcacat aattgggaac
    29761 gttatgaacc gaacccatcc ataaggtaaa atagttaaat gttttccagg tccgctgact
    29821 ttgcgttttc ggcagtagat cttcgctata gccgcgttgc tggaatagtt ttctctgatg
    29881 ttccataaat ccctcttaat ttcacgtatc gaataa
//
LOCUS       DAZKJR010000053        29909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKJR010000053 DAZKJR010000000
VERSION     DAZKJR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.053"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..742
                     /gene="yqiJ"
                     /locus_tag="ACHOWS_002378"
     CDS             113..742
                     /gene="yqiJ"
                     /locus_tag="ACHOWS_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFQ0047938.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            769..2430
                     /gene="yqiK"
                     /locus_tag="ACHOWS_002379"
     CDS             769..2430
                     /gene="yqiK"
                     /locus_tag="ACHOWS_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0047939.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            2670..2729
                     /locus_tag="ACHOWS_002380"
     CDS             2670..2729
                     /locus_tag="ACHOWS_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFQ0047940.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(2849..4282)
                     /gene="hldE"
                     /locus_tag="ACHOWS_002381"
     CDS             complement(2849..4282)
                     /gene="hldE"
                     /locus_tag="ACHOWS_002381"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFQ0047941.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNQ
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4330..7170)
                     /gene="glnE"
                     /locus_tag="ACHOWS_002382"
     CDS             complement(4330..7170)
                     /gene="glnE"
                     /locus_tag="ACHOWS_002382"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFQ0047942.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(7193..8494)
                     /gene="ygiF"
                     /locus_tag="ACHOWS_002383"
     CDS             complement(7193..8494)
                     /gene="ygiF"
                     /locus_tag="ACHOWS_002383"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFQ0047943.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8736..9356
                     /gene="ygiM"
                     /locus_tag="ACHOWS_002384"
     CDS             8736..9356
                     /gene="ygiM"
                     /locus_tag="ACHOWS_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFQ0047944.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9420..10658
                     /gene="cca"
                     /locus_tag="ACHOWS_002385"
     CDS             9420..10658
                     /gene="cca"
                     /locus_tag="ACHOWS_002385"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFQ0047945.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(10846..11667)
                     /gene="bacA"
                     /locus_tag="ACHOWS_002386"
     CDS             complement(10846..11667)
                     /gene="bacA"
                     /locus_tag="ACHOWS_002386"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0047946.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11758..12126)
                     /gene="folB"
                     /locus_tag="ACHOWS_002387"
     CDS             complement(11758..12126)
                     /gene="folB"
                     /locus_tag="ACHOWS_002387"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFQ0047947.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            12231..12848
                     /gene="plsY"
                     /locus_tag="ACHOWS_002388"
     CDS             12231..12848
                     /gene="plsY"
                     /locus_tag="ACHOWS_002388"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0047948.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12861..13793)
                     /gene="ttdR"
                     /locus_tag="ACHOWS_002389"
                     /gene_synonym="dan"
     CDS             complement(12861..13793)
                     /gene="ttdR"
                     /locus_tag="ACHOWS_002389"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFQ0047949.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14000..14911
                     /gene="ttdA"
                     /locus_tag="ACHOWS_002390"
     CDS             14000..14911
                     /gene="ttdA"
                     /locus_tag="ACHOWS_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFQ0047950.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14908..15513
                     /gene="ttdB"
                     /locus_tag="ACHOWS_002391"
     CDS             14908..15513
                     /gene="ttdB"
                     /locus_tag="ACHOWS_002391"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFQ0047951.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15562..17025
                     /gene="ttdT"
                     /locus_tag="ACHOWS_002392"
     CDS             15562..17025
                     /gene="ttdT"
                     /locus_tag="ACHOWS_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFQ0047952.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17068..18081)
                     /gene="tsaD"
                     /locus_tag="ACHOWS_002393"
     CDS             complement(17068..18081)
                     /gene="tsaD"
                     /locus_tag="ACHOWS_002393"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0047953.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18319..18534
                     /gene="rpsU"
                     /locus_tag="ACHOWS_002394"
     CDS             18319..18534
                     /gene="rpsU"
                     /locus_tag="ACHOWS_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0047954.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18645..20390
                     /gene="dnaG"
                     /locus_tag="ACHOWS_002395"
     CDS             18645..20390
                     /gene="dnaG"
                     /locus_tag="ACHOWS_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0047955.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKQAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDNQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20585..22426
                     /gene="rpoD"
                     /locus_tag="ACHOWS_002396"
     CDS             20585..22426
                     /gene="rpoD"
                     /locus_tag="ACHOWS_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0047956.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22589..23584)
                     /locus_tag="ACHOWS_002397"
     CDS             complement(22589..23584)
                     /locus_tag="ACHOWS_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0047957.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRIELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(23651..23944)
                     /locus_tag="ACHOWS_002398"
     CDS             complement(23651..23944)
                     /locus_tag="ACHOWS_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0047958.1"
                     /translation="MDIPQKIRVMRKSEDLTQREFADMIGISYSALTSYEYGRNLPGL
                     EITIKLFKHPRFSKYRDWFLFDEVEPKAGQVVPALAHIGQSEAESSHSGKKTG"
     gene            24060..24485
                     /locus_tag="ACHOWS_002399"
     CDS             24060..24485
                     /locus_tag="ACHOWS_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFQ0047959.1"
                     /translation="MQESTQNAPESLCGTRCDHQSEISEAEHLKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGSRNKRISKKAA"
     gene            24516..25049
                     /locus_tag="ACHOWS_002400"
     CDS             24516..25049
                     /locus_tag="ACHOWS_002400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047960.1"
                     /translation="MIKKYSEHGSKAGGAIAIDEDCLITGDPWLATERDLEGKTFFVR
                     TIKHNDKIVGEIVYSTTTLIPENYLDISFEAVSIQGCNAYLNYLKNLEEIKLDHIWST
                     IKDEIITLQRKGLNPDFSKLPMVIETMKEPKRFHALLDWNLPAREGIDKLVRQHLRTT
                     LLSFDEYLQVQEVITPN"
     gene            25101..25331
                     /locus_tag="ACHOWS_002401"
     CDS             25101..25331
                     /locus_tag="ACHOWS_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047961.1"
                     /translation="MIKHTEIRQRIKISDNASVVICNQNLDAIVRAGNDLYFHYPTHC
                     IVATFETEESLKNAMEQTGFQKETVIRKDNWT"
     gene            25384..25722
                     /locus_tag="ACHOWS_002402"
     CDS             25384..25722
                     /locus_tag="ACHOWS_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFQ0047962.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHRR"
     gene            25742..25984
                     /locus_tag="ACHOWS_002403"
     CDS             25742..25984
                     /locus_tag="ACHOWS_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFQ0047963.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTRDEGLRNSLRGAIMGLYN
                     CALGSDDREALEELKVIVAELFEGEIVEPYFTEVAA"
     gene            25981..26106
                     /locus_tag="ACHOWS_002404"
     CDS             25981..26106
                     /locus_tag="ACHOWS_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047964.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            26186..26464
                     /locus_tag="ACHOWS_002405"
     CDS             26186..26464
                     /locus_tag="ACHOWS_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047965.1"
                     /translation="MSTNTPTEINKLFTDPAHIELTQKTLTEITNVLDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISELYMKNLRLERELAELRAQSGQFSA"
     gene            26476..26718
                     /locus_tag="ACHOWS_002406"
     CDS             26476..26718
                     /locus_tag="ACHOWS_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047966.1"
                     /translation="MNSIFTATMLTRFTDAVGHEFMVESHLITTTTPCPSDADYLYIH
                     LADGTQITAIASTVREVTAIRGAWKSETQAHGELRP"
     gene            26715..26927
                     /locus_tag="ACHOWS_002407"
     CDS             26715..26927
                     /locus_tag="ACHOWS_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047967.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            26954..27508
                     /locus_tag="ACHOWS_002408"
     CDS             26954..27508
                     /locus_tag="ACHOWS_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb domain-containing protein"
                     /protein_id="HFQ0047968.1"
                     /translation="MRLTPMGIPGKDPAHVKAWTQQEDRLLISLYQEHNTHQMAERIQ
                     RTRKAVMYRIRFLRNRGLIGRKRKAPLSTEAIAFLIKNRHVKTAQEMALEVGCSECTV
                     RYTLRKRGYNLKKCGENHHCARYSDHLVELVTELRDIHNMTFCMIAEHINSTIQMHIN
                     DDAAFHLYHRRTATDAVLCELLPG"
     gene            27518..27898
                     /locus_tag="ACHOWS_002409"
                     /pseudo
     CDS             27518..27898
                     /locus_tag="ACHOWS_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27829..28056
                     /locus_tag="ACHOWS_002410"
     CDS             27829..28056
                     /locus_tag="ACHOWS_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFQ0047969.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVTFIMQSNALHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            28125..28535
                     /locus_tag="ACHOWS_002411"
     CDS             28125..28535
                     /locus_tag="ACHOWS_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0047970.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     HVTTYGDFSFTVDGRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            28532..>29909
                     /locus_tag="ACHOWS_002412"
     CDS             28532..>29909
                     /locus_tag="ACHOWS_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFQ0047971.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLYHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATTFSTALERACDEVIEKTG
                     RGDDDLLTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTDPDPERVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMTCARGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRAIDKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSQE"
BASE COUNT         7517 a         8106 c         7517 g         6769 t
ORIGIN      
        1 ctttctcttc cagaacttct ctaacatgtg ttattattaa caaagataaa tgtaagcaag
       61 gaaactatcc taaagtaaat attgcgtcac taaatggaca tcggagtgac atatgatttt
      121 attcgccgac tataacaccc cttatctttt cgctatttcc tttgtactgt tgatcggcct
      181 tttagaaatt tttgcgctta tctgcggtca catgctctcc ggtgctcttg atgcacatct
      241 tgaccattat gattccataa ccacaggtca tattagccag gcacttcatt atctcaatat
      301 cggaagattg ccagctctcg tcgttctctg cttactggct ggtttttttg gtcttattgg
      361 catcctgttg cagcatacct gcctcatggt ctggcaatca ccactctcga acctgtttgt
      421 ggttcctgtc agtttactgt ttacgattat tgcggtgcat tataccggta aaatagttgc
      481 gccctggatt ccacgcgatc acagctcagc tattacagaa gaagaatata ttggtagcat
      541 ggcgttaatt accggtcatc aagccacttc aggtaatccc tgcgaaggaa aactcaccga
      601 tcaatttggc caaatacatt atttactact ggagcctgaa gaaggaaaaa ttttcactaa
      661 aggagacaaa gtgttaatta tttgccgact ttctgcaact cgatatttgg cggaaaacaa
      721 tccctggccc caaatattgt aatttattaa tcaaaaggaa atttaataat ggatgatatt
      781 gttaattctg tgccctcctg gatgtttacc gcgattattg ccgtatgcat tctgtttatt
      841 attggaatta ttttcgccag gctctatcgt cgcgcttcgg cagagcaagc ttttgttcgt
      901 actggtttag gtgggcaaaa agtggtaatg agcggtggcg caatcgtgat gccgatcttt
      961 catgaaataa tccccatcaa tatgaatact ctgaagctgg aagtcagccg ctcaaccatt
     1021 gatagcctga ttacgaaaga tcgtatgcgc gtcgatgtcg ttgtcgcttt ctttgtgcgg
     1081 gtaaaaccgt cagtggaagg tattgccacc gccgcccaga cgcttgggca acgcaccctg
     1141 tcgcctgaag acttacgtat gttggttgaa gataaatttg tcgatgccct ccgtgcaaca
     1201 gctgcacaaa tgaccatgca tgagttacag gatacccgcg agaacttcgt gcagggcgta
     1261 caaaataccg tagctgaaga tctgtcgaaa aacggcctgg aactggaaag cgtttcactt
     1321 accaacttta accagacgtc gaaagaacat ttcaatccta acaacgcctt tgacgccgaa
     1381 ggtttaacta agctgacgca ggagacggag cgccgtcgtc gcgaacgtaa cgaagttgaa
     1441 caggatgtag aagttgcggt gcgtgagaaa aaccgtgatg cgctttcgcg gaagctggag
     1501 attgaacagc aagaagcgtt tatgacgctt gagcaggagc agcaggttaa aacccgtacc
     1561 gctgagcaga atgcgaaaat tgcggctttt gaggccgagc gtcgtcggga agcagagcag
     1621 acgcgaattc tggctgaacg acagattcag gaaacggaaa tcgaccgcga acaggccgtc
     1681 cgttccagaa aagttgaagc tgaacgtgaa gttcgcatta aagagatcga acagcagcag
     1741 gtcaccgaaa tcgccaacca gacgaaatcg atcgcgattg ccgccaaatc ggaacagcag
     1801 tcacaagcgg aagcgcgtgc caaccttgca cttgcagaag cggtaagcgc ccagcaaaac
     1861 gtagaaacca ctcgccagac tgccgaagcc gatcgtgcta aacaagtggc gttgatcgcc
     1921 gcagcgcagg atgcagaaac caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa
     1981 gaagccgcag aaatgcaggc ggcggctatc gttgagttag ccgaagctac acgtaaaaag
     2041 ggtctggcgg aagcagaagc acaacgtgcg ctgaacgatg ctatcaacgt actttctgat
     2101 gaacaaacca gccttaaatt caaactggca ttattgcagg cgctgcctgc ggtaattgag
     2161 aaatccgtgg aaccaatgaa gtctatcgac ggcatcaaaa ttattcaggt cgatgggctg
     2221 aatcgtggtg gcgctacggg tgatgcaaac acgggtaatg tggggggcgg aaacctggca
     2281 gaacaagcgt tatcagccgc actctcttac cgtacacagg caccgctgat tgactccttg
     2341 ctcaatgaaa ttggcgtttc aggcggctca ctggcggcat tgacttcatc cttaagctca
     2401 acaactcccg tcgaagaaaa agcagagtaa accctcttct atgaataatc cctggcccaa
     2461 aaagccgggg attttttata tctgcgttcc gctaaaaggt gcaaatgatc aagctgttgc
     2521 agcattttgc gtgactgctc ggggaaggca aaactgcctc tgggaaagcg ttgcgcgggg
     2581 tccggcgctc atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg
     2641 aggaaggggc cttgtataag gaaagggtta tgatgaagct cgtcatcata ctgattgtgt
     2701 tgttactcgt aagtttcgca gcttattaac agccaatcag aggggggaga aatcctccct
     2761 cacccttgtt cctttacttt aggttgaaaa aaaatcagcg tcaataggcc cgcctgctac
     2821 aaagcgagat ctgtgaaccg ctttccggtt agcctttttt atcctgttgg atcttcttga
     2881 tgatgttggt cgtcgagcaa ccgtcttcaa agttgagcac cagcacttca ccaccgttgg
     2941 cccagacttc tttactcccg gcaatctctt ctggtttata gtcgccgcct ttcaccagta
     3001 gatctggcaa gataccggcg atcaagcgct gcggcgtgtc ctcttcaaac gacaccaccc
     3061 agtcgaccgc ttccagtgcg cccagcacaa tcatacgctg ttcgagctgg ttaacggggc
     3121 gggaatcccc tttcagccgt ttggtggagg catcgctgtt cacagcgaca atcaaacggt
     3181 cacccagttt acgggcattt gccagataag agacgtgccc agcgtgcagg atgtcaaaga
     3241 caccgttggt catcaccact ttttcaccgc gtttacgcgc tgccgctaca gcaagcttca
     3301 gttcctcttc ggtcatcaca ccaaagccgg tatctgcacg tccacgtaca gcgttttcca
     3361 gctcgatcgg cgaaaccgtg gatgtcccca gtttgccgac caccacacca gccgccgcat
     3421 tggcaaagaa gcaggcttct tccagtgaat tacccgctgc cagcgttgcc gccaggacgc
     3481 caatcaccgt gtcgcccgca ccggtaacgt catacacttc ctgcgcctgg gttggcatat
     3541 gcagtggcgc tttacccggt tgcagcagcg acataccctg ttcggaacgg gtcactaaca
     3601 gagccgagag ttcgtaatcg gcaatcagtt tcatgccgcg ctcaacaatc tcttcttcgg
     3661 tcttacattt accgacaaca gcttcaaatt ccgagagatt cggcgttaac agcgtagcgc
     3721 cgcggtagcg ctcaaaatcg gtaccttttg gatcaatcag caccggaaca cccgctttac
     3781 gcgccagttg gatcatctgc tgtacgcttg ccagcgcacc tttggcgtaa tcagaaagca
     3841 ccagcgcgcc aatcgaactc agtgcctgat taatccgctc gtgcagcggc tgcggatcaa
     3901 ccccttcgaa accttcttcg aaatccagac ggatcagttg ttggttgcgg gaaagtaccc
     3961 gtaatttggt gatggtcgga tgcgtcggta cagaaacaaa gtcgcatttg acgttgacgt
     4021 cggccagaga tttactgagc gcgcgcgccg catcgtcaat gcccgtcaac ccgaccaggc
     4081 gtgcattagc accgagagaa gcgatattca tcgccacgtt agccgcgccg cccggacgtt
     4141 cttcgatggt attcacttta accacgggca ccggcgcttc cggcgagata cggctggtgg
     4201 gaccgtacca gtaacgatcc agcatcacat caccaaccac catcactcct gcacgttcaa
     4261 actctggcag cgttactttc attcctgtct cctgagagat tcaaaatttg cgcgcgataa
     4321 taccatactt cattcttcca ccagccactt ctgccagctt gcccgcacca gttcacgctc
     4381 tgcggtgaag caatcctccg acacatgacc cggcaattcc tgtaatgcca gatgatgaag
     4441 ttcatcgcgc agcgtcgtgt aagcacgggt cagcgccatc gcttcctgct cttccataat
     4501 gtcgttttgc gccagtagtt ccagaatgcg cacgttgtct gaccagcgcg ttaacttcgg
     4561 tttttcgtga gcgtagcgca acaccagata ttgagtaata aattcgatat cggtaattcc
     4621 gccttcatcg gctttgatat caaagcgatc gcgatgttta ttgccgagat gggcacgcat
     4681 tttctcgcgc atttcccgca cttctgtttg cagggttttg ccttcgcgcg gcagcgtcat
     4741 aatctcccgg cgtactgcgt caaagtgcgc ggtgagctgc ggatcgccgt acactacacg
     4801 cgcacgcacc agcgcctgat gctcccacgt ccaggcctcg tttttctgat aatcagcaaa
     4861 tgcttctgcg gatgtcacca gcattcccgc cgccccggac ggacgcagtc gtgcatccac
     4921 ttcatacaaa atgccggaag aggttcgcgt actgaacaga tgcataatgc gttgcgccag
     4981 acgcagataa aactgtcgcc cgtcgatttc ccgctcgccg tcagtcatcg catccattgg
     5041 gcaatcatgg aggaagatca ggtcaagatc ggaactgtag cctaactccc agccacccag
     5101 cttgccgtag ccgaccaccg caaaaccacg cccttcgcgt tcgttcaggt gatttggctt
     5161 accgtagcgg gcaaccattt gaacccacgc ctgctgaacg acggcatcaa tcatggcttc
     5221 tgccagccag gttaagtgat cgctcacttt cattaccggt agcgtaccgg cgatatccgc
     5281 tgccgcaatg cgtaacagct gcgcctgttt gaactgacgc agcgcctcaa gctgttgttc
     5341 ttcgtcatct tccggcacgc gcagcaaata ctggcgcagt tcatcacggt aggcatccgt
     5401 cgccgttggt tggtagaggg tatttggatc gagcaattca tccagcaata atggataacg
     5461 cgctagctgg ctggcaatca tcggcgatgc agcacacaga gaaatcaaat gtttaagcgc
     5521 cgcagggaat tcactaagca gctcaagata agtggtgcga gtaacaatcc ccaccagcaa
     5581 ggcggtaatg cgcgacagcg tgacagcagc gtcttcacgc gcacagacat cacttagcag
     5641 atgtggcatc agatggtcga gcacctgacg tcctcgcggc ccgatggtgc gcttatccag
     5701 ctctttgcgg aaatcagcaa tcagcattag cacctgtttg cgatcatcct cgctaagatg
     5761 cgccagcact ggcgtggtgt catcttcctg caacgcatcc tgccacagct cacgccactg
     5821 ttccgacagc gactcttcct gagtttcact ttcgtcgtcg ccaatcaatt cattaaacac
     5881 ccggcgcaca ttggccatat gtgcggtcag caccccggtc aattgcggcc agtcagcaaa
     5941 gtccatcgcc cacgccagcc gcgcacggtt aagctcatca aaaggaagcg tctgggtttg
     6001 ttcgtcgtta atgctttgca gcaggttttc cagacgccgc aggaacagat acgccactcg
     6061 caattgttca gcatcgtttt cagaaagcag atgcagcgcg gcaatggcgc tgagcgttgg
     6121 cagtaaagag cgcgattgca gcgacggttc gcgcccgccg cgaatgagct ggaacacctg
     6181 gacgataaat tcaatttcgc gaatgccgcc tgcgccaagt ttgatgttgt cggtcaaacc
     6241 gcgtcgacgc acttcacggg caatcattcc tttcatgttg cgcagcgact gaatcacgct
     6301 gaaatcgatg taacgacgga aaacaaacgg acgcagcatc gcacgcaact cgttagcata
     6361 gacgccgtcg ctatccccca taatccgcgc cttgaccatc gcgtaacgct cccagtcgcg
     6421 cccctgctcc tggtaataat cttccagcgc cgcaaaactc agcaccagcg ggccactttc
     6481 accaaacgga cgcagccgca tatccacgcg atagacgaaa ccatcctgcg ttggttgatc
     6541 cagcacttta atcagccgct gccccatgcg ggtaaaaaac tgcgcgttat ccagttcccg
     6601 gcgtccgccc tgggtacaac catgttccgg ccaggcaaaa atcagatcga tatctgagga
     6661 gaaattcagt tccccaccgc ccagcttacc catgcctaaa atcagcagcg gttgcgcttc
     6721 gccctgcgca ttgcacggcg ttccccattc gcggcagcag gcgtcataca gccagtcacg
     6781 cgccgcaaca atcagcgtct ccgccagatg gctgagctgc tgcaatatgc tctcttcagt
     6841 aaccagtgcc agcgtttgcg cccaggcgat gcgcaccata atgcgccgcc ggaatagccg
     6901 cagctcgcgc attaacccgg cttcgtcact cacattactg agcgcctcct gcaaccatgc
     6961 tgcgtaatgc tgccattcgt cggcctgcgg cggttggctt tccagttccg tcagccactc
     7021 tggatgcgca attatgctgt cctgcagaaa atcactgaaa gtaagtactg acttcgcctg
     7081 tgcgctaagt gattcctcgg ctaaaggctc tggcagccgc tcaacaacgg tctgccagta
     7141 ctgctgtaac ggtgaagaga gcggcttcat aagcgatttt atccttgcct gattaacgtt
     7201 ttccgctgtg caaccagaac ggttcctgat tgtttgcctc attacggaaa tgttcaattt
     7261 caatgcgttg cccggtcgca atagcgtgac gcagcccctg ccagttctcc agccaggctt
     7321 gcgcgacgac aggatcgtag taacccgcca gcaacaggat tgagtcaata tcacgcgtca
     7381 ggcgtggcaa ctggtcacgg tagcgatcgc ccaacggctg gcagaaaacg cttttcagtt
     7441 cagcggcatg gcgggaaaga tggatatcgg caaagcgttt gaaggaatcg ctcattttac
     7501 tctgcgcttt ggcatctaaa aacggctgcc aggctttgct taccagccat tcggtcaatg
     7561 ccagcttcgc cattgccgtt tcggtagagt agaccgccgt cacggcagaa accgctgaag
     7621 cgatggtcgc ttcgcattga gtcaataaat cacgtaagtg agtgctcgct ttacgcggca
     7681 caatgccacc gaacagcatc agcgtatgac ggaccaggcc aatggctgcc agcacctgct
     7741 ctttcgccgc atcattgccg cgtacccata gttcttcatg atactgccat tgcgctaacg
     7801 ccagctcgag cgccgcttcc agcccctgtt ccacatcagc ttttgccgca acatgtaaaa
     7861 tggtggtcgg tttgatttca cgcgccggat tgccctgcgc cagatgatag ccacgcgccg
     7921 ctttgctcag actgccctgg cgtaatccgg tttgcgatac cagttggttc gccagtttca
     7981 gcaccgcgcg cgtgtcgccg ctaagcagtt ccagttccag ctcgcagata ggttcagcaa
     8041 attcacccgc tttcacttcc ccctggtcga gggcgatttc aatttggcta ccatcaacct
     8101 ctaccagcca cttttcgcga taaaaatcgg tgctaaacag cggctgcacg cgggaagcga
     8161 gatcggtagg caattcgccg ttcggccaga cttccgtcgg taactgcgcc aggtcgagcg
     8221 tcggttcgct caacgccacg ttatattccg ggcgctggtg taagccgcct gtcactcttc
     8281 ctgcaacttt catggtcatc tcatagcgac cgttttcgcc acgaatacgc aagcccatgt
     8341 cgtgcccacg cagccagtta tccggcgttt cgtagtaaat attcagcaac tgcacggggt
     8401 catggtgctc gccgcccagc gtattgagat ggtcacgcaa cgcctcaacg gcactgtgat
     8461 taacaataaa ctttaattcg atttcctgag ccatagcctt tacttatcgg ttatgtcaca
     8521 taagtgacga tgaacggcga acttaatgcg atcttttttg tcagtagata gtattttgcg
     8581 ccaaattgcc atgcaacggg caatttgacg ggcgtaaaag tttgaagcag tggcaagacc
     8641 tgacacagag gattccgatt gatgtcgaat gctttgcgta gcgacactga tgccactact
     8701 atcgttccac tttccatgac aataacgaca gcctgatgcc aaaattacgc ctgatcggat
     8761 taactttact cgcacttagc gccactgccg tctcacacgc tgaagaaacg cgctatgttt
     8821 ctgatgaact gaatacctgg gtccgtagcg gtccgggaga tcattatcgc ctcgtgggca
     8881 cggttaacgc cggcgaggaa gtgaccttat tacaaactga cgccaacacc aattatgccc
     8941 aggtgaaaga cagctctggc cgtaccgcct ggatcccgtt gaaacaactt agcacagagc
     9001 caagcctgcg ctcccgtgtg ccagatctgg aaaatcaggt caaaaccctg accgataaac
     9061 tcaccaatat cgataacacc tggaaccaac gcaccgcaga aatgcagcaa aaagtggcgc
     9121 agagcgacag cgtgatcaac gggttaaaag aagaaaatca gaaactgaaa aacgagctga
     9181 ttgtcgcgca gaaaaaggtc gatgccgcca gcgtacagct ggatgacaaa cagcgcacca
     9241 tcatcatgca gtggtttatg tatggcggtg gcgtgctggg gcttggcttg ctgcttggtc
     9301 tggtactgcc gcacctgatc ccaagccgca aacgcaaaga tcgctggatg aactaaatcg
     9361 ccttcttcgt tgcactgtct tattattagg aattgtgtat ttgacgaagg gatgatggcg
     9421 tgaagattta tctggtcggt ggtgctgttc gggatgcatt gttagggcta ccggtcaaag
     9481 acagagattg ggtggtggtc ggcagtacgc cacaggagat gctcgacgcg ggctaccagc
     9541 aggtaggccg cgattttcct gtgtttctgc atccgcaaac gcatgaagag tatgcactgg
     9601 cacgtaccga acggaaatct ggttccggtt acaccggttt tacctgctat gccgcaccgg
     9661 atgtcacgct ggaagatgat cttaagcgtc gcgatctgac cattaatgcg ctggcccagg
     9721 acgataacgg cgagattatc gacccgtaca acggtctggg cgatctgcaa aatcgtctgt
     9781 tgcgccatgt ttcccccgct tttggcgaag atccgttacg cgtgttgcgc gtagcgcgtt
     9841 ttgctgcgcg ttatgcccac ctcagtttcc gtattgccga tgaaactctg gcgctgatgc
     9901 gcgagatgac ccatgcgggt gaactggaac acctgacgcc tgaacgggta tggaaagaga
     9961 cggaaaacgc cctcaccacc cgcaatcccc aggtgttctt ccaggtactg cgcgattgcg
    10021 gcgcactgcg cgttttattc ccggaaattg acgccctgtt tggcgttccg gcccctgcca
    10081 ggtggcatcc ggaaatcgac acgggtattc ataccttaat gacgctctca atggcggcga
    10141 tgctgagtcc gcaggtcgat gtccgtttcg caacgttatg ccacgatctc ggtaaaggac
    10201 tgacgccacc agaactttgg ccgcgtcatc atggtcatgg cccggcgggt gtgaagttag
    10261 tggaacaact atgccagcgt ctgcgcgtgc caaatgaaat tcgcgattta gccagactgg
    10321 tggctgagtt tcacgatctc atccacacct tcccaatgct gaacccaaaa accatcgtca
    10381 aactatttga ttccatcgac gcctggcgta aaccgcagcg tgtcgagcaa ctggcgctga
    10441 ccagcgaggc tgatgtgcgc ggcagaaccg gttttgaatc agccgactac ccgcaaggcc
    10501 gctggttgcg cgaagcctgg gaagtggcac agtcagtgcc gacaaaagcc gtcgttgaag
    10561 cgggatttaa aggtgtggag attcgcgagg agctgacccg acggcggatt gcggcggtgg
    10621 ctggctggaa ggaacagcgt tgcccaaaac ctgagtgagt gttggatatt ttgccggatg
    10681 cgacgtaagc gtcttatccg gctacatgtc aacgacagtt gtaggcctga taagacgcgc
    10741 gagcgtcgca tcaggcgttg attgccggat gcggcgtgaa cgccttatcc ggcctacgca
    10801 atctgataag acgcgtcagc atcatatccg acactgttct tcacatcaaa agaacacaac
    10861 gtacaccgca gccgccacaa taaagcgata aatggcgaac gggataaacg aaatgcgctt
    10921 aatcaattgc aggaaggttt taatcgctat cagcgccacc acaaaagcgg tgataaaccc
    10981 aacggcaaac atcgggatat cgccggtagt caggaagccc cagcttttgt agagatcgag
    11041 cgccgttgcg cccatcatca tcggcaccgc cagcaggaac gagaactcgg aagcagcgta
    11101 acggctcacc cccatcagca tcccacctga aatggtcgcc ccggaacggg agaaacccgg
    11161 ccacagcgcc agacactgga aacagccaat cataaacgcc tgacgatagg tcatatcatc
    11221 aagacccggc gcacgcggct ctttcggctt caggcattcg gcggcaatca gcaacagacc
    11281 gccaacgacc agtgcataca tcacatttat cgggttaaac aatgatttaa tcgtgtcgtg
    11341 gaacaacagc cccagtacca ccgccggaat catccccagc aaaatgtgga tcagcgttaa
    11401 acggcctttg ctttctcctt cgtgctgcaa cgggcgacca aagtgaatac caataaggcc
    11461 aaacagacgt cgccagaaca tgacaaccac tgccagaatt gatcctaact ggatcacgac
    11521 ttcaaaggtt ttcgccgtgt cgccctcaaa ccccaacaag tgaccgacaa taatcatatg
    11581 gcccgtgctg gataccggca gaaattctgt caatccttcg accacaccca atattgccgc
    11641 tatcagcagc gagtgcatat cgctcatcaa taaaccccta aattataaaa atgtaacgct
    11701 tgtccgaact actgcagtat gaccaggtta taaccgtttg gtttaacagc tgtaaaatta
    11761 attattttct ttcagattat tgccacgctc aatgattacg ccaacattcg ccgcccgcgc
    11821 cactgcgcct ggcttgctga gtttgatacg cacccacggc gagttgaagc gtgctaacag
    11881 caactccgcc acctcttcag ccacacgttc caccagcgca aaacgcgccc cctcgacgtg
    11941 gctgactacc gtttctgcaa tgtcagcgta actgaggcaa tccgccacat catcactttt
    12001 cgccgcttta cggttatccc acgccatttc gatatcgaac actaacttct gttcgatggt
    12061 ctgttcccag tcgtaaacac caatagtggt gattaccgaa agttgctcta taaatacaat
    12121 atccatcacg tcctgcctgc tttttggcta accggatacc acttccggcg aaatgtgcgt
    12181 attatccaca gattcatcgt tgaacacgaa ttttcaaaac ggaacagctt atgagtgcaa
    12241 tcgcgcctgg aatgatcctc atcgcgtacc tctgcggctc catttccagt gccattctgg
    12301 tttgccgctt gtgtgggctg cccgatccgc gaaccagcgg ctccggcaat ccaggcgcaa
    12361 ccaatgtgtt acgtatcggt ggcaagggag cagccgtagc agtactgatt ttcgacgttc
    12421 tgaaaggaat gttgcccgtc tggggcgcgt atgaattagg tgtcagcccc ttctggctag
    12481 gcttaattgc catcgccgcc tgtcttggac acatctggcc cgttttcttc ggatttaaag
    12541 gaggaaaagg cgttgctacc gcttttggtg ccatcgcacc cattggctgg gatctcaccg
    12601 gagtaatggc gggaacctgg ttactgaccg tgctattgag cggatattcg tcgctggggg
    12661 cgattgtcag tgcactgatt gctccgtttt atgtctggtg gtttaagcca caattcacct
    12721 tcccggtttc gatgctctct tgcctgatcc tgctgcgcca tcatgacaac atccagcgtc
    12781 tgtggcgtcg tcaggagaca aaaatctgga cgaaattcaa aagaaagcgc gaaaaggatc
    12841 ccgagtgatt tctggtggag ctacataacc tgatagcctt cgtcgggctt gcccagccgt
    12901 tgctggcacc atgccgccag aaattccacg cagacacgta atttcatgct gcgatagagc
    12961 ggctcccggt aaacagccca gatattggcg ctttgtgcat actccggcaa tactcgcacc
    13021 agtttgccac tctccagaaa cggcaacaca tcccactcgg aacgcaacat aatccctttg
    13081 ccctccagcg cccattgcaa cactatctcg ccgctattgg aggaaagatg cccgcttacc
    13141 ttcaccgatt ttttctcctg accgttcccc aactcccata ttccgtgggt catatcgcgc
    13201 tctttggtca ccaggcagtc atgacgactt aattcctgta aggattgtgg ctgcggatat
    13261 ttttgcagat actcaggtgc tgcgcataat attcttttat tttttgtcaa aagatgcgca
    13321 atataataat caggaattgc gtcattaata cgaatatcca gatcaatatt atcctgcact
    13381 aaatcaattt gccgatcgaa caattcaaaa tgcacctgta actcaggata attgcgcatc
    13441 agttcggtaa tggctggagc aatatggctg cgcccaaaac caaaactgca accaatgcgg
    13501 atcatcccct ccgggcgcgt tttgatttgc gtgacgtcat cgaccagtcg ctgatattgg
    13561 gtgaggattt ccagcgcatg ttcgtaacaa cgttggccac tttcagtcag cgccacgccg
    13621 cgagcggagc ggttcagcag agttgttgcc agcgtatttt caaggatttg gatccgcttg
    13681 gtgacaaatg caggcgtttg cccaagcgtc gccgctgcag cactaaaact cccgctatgg
    13741 acgatttcca ccagtacctg taagtctttg gctaaaggcc agctatttag cattgttaaa
    13801 tatccgcagt tttgataaac gcagtgtatt tcgcaaaaca ttaccattcc ctgctctggc
    13861 gcaatattcg ttcttatttt cgcgttgatt cacgaactgt taattacata ttcacggtgg
    13921 caaaaaatat aaaaccacat ttttagtggt agtttgtggc ggtgaatttt tccagacaaa
    13981 tacataaact ggagttgcca tgatgagcga aagtaataag caacaggcag tgaataagtt
    14041 gacggagatt gtcgctaact ttaccgccat gatttctacc cgaatgcctg atgacgtggt
    14101 ggataaacta aaacagctaa aggatgccga aacatcgtcg atgggaaaaa tcatctacca
    14161 cacgatgttc gataacatgc aaaaagcgat cgacctgaat cgtcctgcct gtcaggacac
    14221 cggcgaaatc atgttttttg ttaaggtcgg ttcccgtttc ccactgcttg gcgagctgca
    14281 aagcatcctc aaacaagccg tggaagaggc aaccgtcaaa gcgccactgc gtcacaatgc
    14341 ggtagaaatt tttgacgaag tgaacaccgg caaaaatacc ggcagcggcg taccgtgggt
    14401 cacctgggat atcgtccccg acggtgacga tgcggaaatc gaagtttaca tggcaggcgg
    14461 cggctgcaca ctacctggtc gttcgaaagt gttaatgccg tcggaaggat acgaaggcgt
    14521 ggtgaaattc gtcttcgaaa atatctccac cctcgccgta aacgcctgtc caccggtact
    14581 ggtaggcgtg ggcatcgcca cctcggtgga aaccgccgct gtgctctcac gtaaagccat
    14641 tttacgccca attggcagcc gccaccccaa tccgaaagcg gcagagctgg agctacgcct
    14701 ggaagaagga ctcaaccgtc tggggattgg tccacaaggg ctgactggca acagttcagt
    14761 gatgggcgta catatcgaat ctgccgcccg ccatccgtca accatcggcg ttgctgtttc
    14821 tacaggctgc tgggcgcatc gtcgcggcac actgctggtt catgccgatc tcacctttga
    14881 aaatctgtct cacacccgga gcgcgttatg aaaaagatcc tgacaacccc gatcaaagct
    14941 gaagatctgc aagatattcg cgtcggcgat gtgatctacc tgaccggtac gctggtgacc
    15001 tgccgtgacg tttgtcaccg tcgcttgatc gaactcaaac gtccgatccc ttacgatctc
    15061 aacggcaaag cgattttcca cgctggcccc atcgtgcgca aaaacggcga caaatgggag
    15121 atggtatccg tcggcccgac aaccagtatg cgtatggaaa gttttgaacg tgaatttatc
    15181 gaacagaccg gcgtgaaact ggtggtcggc aaaggcggaa tggggccgct gaccgaagaa
    15241 ggctgccaga aattcaaggc gctgcatgtg attttcccgg caggctgcgc agtactggcg
    15301 gcaacccagg tggaagagat tgaagaagtg cactggacag aactcgggat gccggagtcg
    15361 ctgtgggtct gccgggtcaa agagttcggt ccgctgattg tttctattga tacccacggc
    15421 aacaacctga tagccgaaaa caaaaagctg ttcgccgaac gccgcgatcc catcgtggaa
    15481 gagatttgcg agcacgtcca ctacatcaaa taaccctccc ggagaggctc accccctctc
    15541 ctttttcgca ggcataacac gatgaaacct tccactgaat ggtggcgata ccttgcgccg
    15601 ctggcggtca tcgccattat tgctctaatt ccggttcccg cagggctgga gagtcatacc
    15661 tggctctact ttgccgtttt tactggcgtg atcgttggac tgatcctcga acccgtgccg
    15721 ggtgccgtgg tggcgatggt gggtatctcc attatcgcca ttctctcgcc gtggctgctg
    15781 tttagcccgg agcagctcgc ccagccaggt tttaagttca ccgccaaatc cctctcctgg
    15841 gcggtttctg gtttttctaa ttcggttatc tggctgattt tcgccgcctt tatgtttggc
    15901 acaggctatg aaaaaaccgg gcttggacgc cgcattgcgc tgattctggt gaaaaagatg
    15961 ggacatcgca cgctgtttct cggctatgcg gtgatgttct ccgagctgat cctggcacct
    16021 gtaacaccgt ccaactcggc gcgcggtgcg gggattatct atcccatcat ccgtaacctg
    16081 ccaccgctct atcaatcaca accaaacgac agcagttcgc gcagcattgg ctcgtacatc
    16141 atgtggatgg ggattgttgc cgactgcgtg accagcgcca ttttcctgac ggcgatggca
    16201 cctaacttgc tgttaattgg cctgatgaaa agcgcatctc acgccacact gagttggggc
    16261 gactggttcc tcgggatgtt gccgctcagt attttactgg ttctgctggt tccctggctg
    16321 gcttacgtgc tgtacccgcc ggtactgaag tctggtgatc aggtgccgcg ctgggcagag
    16381 acggaactgc aggcaatggg cccgctctgt tcgcgtgaaa aacggatgct ggggctgatg
    16441 gtaggcgcgc tggtgctgtg gattttcggc ggtgattata tcgatgccgc gatggtcggt
    16501 tacagcgtgg tggcgctgat gctgcttctg cgcattatca gttgggacga cattgtcagt
    16561 aataaagccg cgtggaacgt tttcttctgg ctggcctcgc ttatcaccct cgctaccgga
    16621 ctcaacaaca ccggttttat tagctggttt ggcaaactgt tagcaggcag cttaagcggt
    16681 tattcgccaa cgatagtgat ggtggcgttg attgtggtgt tttatctact gcgctacttt
    16741 ttcgccagcg ccacggcgta tacctccgct ctcgcgccga tgatgattgc cgccgcgctg
    16801 gcgatgccgg aaatcccgct gccggtgttc tgcctgatgg ttggcgcggc aattggtctg
    16861 gggagcattc ttacgccata cgccaccgga cccagtccga tttactacgg tagtggttat
    16921 ctgccaacgg tggattactg gcgactgggg gcaatttttg ggctgatatt cctcgtgttg
    16981 ctggtgatta ccggcttact gtggatgccc gtggtgttgc tttaagtgag gacggtgccg
    17041 gaagtgactc tccggcacct aagagtttta cgcggccggt aactctgcca gcggccagcg
    17101 cggacgcacg ctaacgccaa gatccgccgt cgcgcctgct ttaaaccgca ccattccggc
    17161 ataggcgatc atcgcgccgt tatcagtaca aaattccgga cgcgcgtaga acacttcgcc
    17221 acggcgtttt ttcatcattt ccgccagctt cgcccgtaac gtgcggttag cactcacgcc
    17281 acctgccatt accagtcgct taaagcccgt ctgatccagc gctcgcttac acttaatcat
    17341 caacgtatcg accaccgcat cttcaaaggc gcgggcgata tcagcacgcg tctggtcgtc
    17401 ggtgccgtta tcacgaatgg tatttgccgc gaaggttttc agaccagaaa agctgaaatc
    17461 cagccctgga cggtcggtca tcgggcgcgg gaagacaaag cgcccggctg taccctgagc
    17521 cgccattttc gacagtaatg gcccgccagg ataatccagc cccagcagct tcgcggtttt
    17581 atcaaacgct tcaccagccg catcatcgat agactcgccg agcagctcgt actgaccaat
    17641 gccagtcacg ctgattaact gcgtatgacc gccggaaacc agcagcgcca caaacggaaa
    17701 ttccggtggg ttatcttcca gcatcggcgc taacagatgc ccttccatat ggtgtacagg
    17761 gatcgccgga acgttccagg caaacgccag agaacgcccc acggtcgcgc caaccagcag
    17821 tgcgccgact aatccagggc ctgcggtata ggccacagca tcaatgtctt ttgccgttaa
    17881 accagactcc tttagcgccg cctggatcaa cggtacggtt ttacgcacat gatcgcggga
    17941 ggccagctca ggcactacgc cgccgtagtc agcgtgcaat ttcacctgac tatacaattg
    18001 gttggctaac aaaccttttt catcgtcgta aatggcgatg ccggtttcat cgcaggaagt
    18061 ttcaataccc agtacacgca tgacttgttt tacctcgctt tattaccgcg cagtgtagga
    18121 ccaatgcggg ttgatgtaaa actttgttcg cccctggaga aagcctcgtg tatactcctc
    18181 acccttataa aagtcccttt caaaaaaggc cgcggtgctt tacaaagcag cagcaattgc
    18241 agtaaaattc cgcaccattt tgaaataagc tggcgttgat gccagcggca aaccgaatta
    18301 atcaaaggtg agaggcacat gccggtaatt aaagtacgtg aaaacgagcc gttcgacgta
    18361 gctctgcgtc gcttcaagcg ttcctgcgaa aaagcaggtg ttctggcgga agttcgtcgt
    18421 cgtgagttct atgaaaaacc gactaccgaa cgtaagcgcg ctaaagcttc tgcagtgaaa
    18481 cgtcacgcga agaaactggc tcgcgaaaac gcacgccgca ctcgtctgta ctaattcccc
    18541 gagagcgttg ctctccgatc agaccgagtt gtagttgtaa ggccgtgctt ccgaaaggaa
    18601 tgcgcggctt attttcgttt atgaattgct aaaaatcggg gcctatggct ggacgaatcc
    18661 cacgcgtatt cattaatgat ctgctggcac gcactgacat cgtcgatctg atcgatgccc
    18721 gtgtgaagct gaaaaagcag ggcaagaatt tccacgcgtg ttgtccattc cacaacgaga
    18781 aaaccccgtc attcaccgtt aacggtgaga aacagtttta ccactgcttt ggatgtggcg
    18841 cgcacggcaa cgcgatcgac ttcctgatga actacgacaa gcttgagttc gtcgaaacgg
    18901 tcgaagagct ggcggcaatg cacaatcttg aagtgccatt tgaagcaggt agcggcccca
    18961 gccagatcga gcgccatcaa cgacaaacgc tttatcagtt gatggacggt ctgaatacgt
    19021 tttaccaaca atctttacag caacctgttg ccacgtctgc gcgccagtat ctggaaaaac
    19081 gcggattaag ccacgaggtt atcgcccgct ttgcgattgg ttttgccccc cctggctggg
    19141 acaacgtcct gaagcggttt ggcggcaatc cagaaaatcg ccagtcattg attgatgcgg
    19201 gcatgttggt cactaacgat caggggcgca gttacgaccg tttccgcgag cgggtgatgt
    19261 tccccattcg cgataaacgc ggtcgggtga ttggttttgg cgggcgtgtg ctgggcaacg
    19321 atacacccaa atacctgaac tcgccggaaa cggacatttt ccataaaggc cgccagcttt
    19381 acggtcttta tgaagcgcag caggataacg ctgaacccaa tcgtctgctt gtggtcgaag
    19441 gctatatgga cgtggtggcg ctggcgcaat acggcattaa ttacgccgtt gcgtcgttag
    19501 gtacgtcaac caccgccgat cacatacaac tgttgttccg cgcgaccaac aatgtcattt
    19561 gctgttatga cggcgaccgt gcaggccgcg atgccgcctg gcgagcgctg gaaacggcgc
    19621 tgccttacat gacagacggt cgtcagctac gctttatgtt tttgcctgat ggcgaagacc
    19681 ctgacacgct ggtacgaaaa gaaggtaaac aagcgtttga agcgcggatg gagcaggcga
    19741 tgccactctc cgcatttctg tttaacagtc tgatgccgca agttgatctg agtacccctg
    19801 acgggcgcgc acgtttgagt acgctggcac tgccactgat atcgcaagtg ccgggcgaaa
    19861 cgctgcgaat atatcttcgt caggaattag gcaacaaatt aggcatactt gatgacaacc
    19921 agcttgaacg attaatgcca aaagcggcag agagcggcgt ttctcgccct gttccacagc
    19981 taaaacgcac gaccatgcgt atacttatag ggttgctggt gcaaaatcca gaattagcga
    20041 cgttggtccc gccgcttgag aatctggatg aaaataagct ccctggactt ggcttattca
    20101 gagaactggt caacacttgt ctctctcagc caggtctgac caccgggcaa cttttagagc
    20161 actatcgtgg tacaaataat gctgccaccc ttgaaaaact gtcgatgtgg gacgatatag
    20221 cagataagaa tattgctgag caaaccttca ccgactcact caaccatatg tttgattcgc
    20281 tgcttgaact gcgccaggaa gagttaatcg ctcgtgagcg cacgcatggt ttaagcaacg
    20341 aagaacgcct ggagctctgg acattaaacc aggagctggc gaaaaagtga tttaacggct
    20401 taagtgccga agagcgatcg ggaagccccc gacagccgca ctgagaggca gcggcaaata
    20461 tataagtacg ccctcgtaat tatcgttggc ggtaaacaac cgttggattt cagcgttaac
    20521 ggctgaagga catcgggtca atcgcccaac accaacctca tgaaataagt gtggataccg
    20581 tcttatggag caaaacccgc agtcacagct gaaacttctt gtcacccgtg gtaaggagca
    20641 aggctatctg acctatgccg aggtcaatga ccatctgccg gaagatatcg tcgattcaga
    20701 tcagatcgaa gacatcatcc aaatgatcaa cgacatgggc attcaggtga tggaagaagc
    20761 accggatgcc gatgatctga tgctggctga aaacaccgcg gacgaagatg ctgccgaagc
    20821 cgccgcgcag gtgctttcca gcgtggaatc tgaaatcggg cgcacgactg acccggtacg
    20881 catgtacatg cgtgaaatgg gcaccgttga actgttgacc cgcgaaggcg aaattgacat
    20941 cgctaagcgt attgaagacg ggatcaacca ggttcaatgc tccgttgctg aatatccgga
    21001 agcgatcacc tatctgctgg aacagtacga tcgtgttgaa gcagaagaag cgcgtctgtc
    21061 cgatctgatc accggctttg ttgacccgaa cgcagaagaa gatctggcac ctaccgccac
    21121 tcacgtcggt tctgagcttt cccaggaaga tctggacgat gacgaagatg aagacgaaga
    21181 agatggcgat gacgacagcg ccgatgatga caacagcatc gacccggaac tggctcgcga
    21241 aaaatttgcg gaactgcgcg ctcagtacgt tgtaacgcgt gacaccatca aagcgaaagg
    21301 tcgcagtcac gctgccgctc aggaagagat cctgaaactg tctgaagtat tcaaacagtt
    21361 ccgcctggtg ccgaagcagt ttgactacct ggtcaacagc atgcgcgtca tgatggaccg
    21421 cgttcgtacg caagaacgtc tgatcatgaa gctctgcgtt gagcagtgca aaatgccgaa
    21481 gaaaaacttc attaccctgt ttaccggcaa cgaaaccagc gatacctggt tcaacgcggc
    21541 aattgcaatg aacaagccgt ggtcggaaaa actgcacgat gtctctgaag aagtgcatcg
    21601 cgccctgcag aaactgcagc agattgaaga agaaaccggc ctgaccatcg agcaggttaa
    21661 agatatcaac cgtcgtatgt ccatcggtga agcgaaagcc cgccgtgcga agaaagagat
    21721 ggttgaagcg aacttacgtc tggttatttc tatcgccaag aaatacacca accgtggctt
    21781 gcagttcctt gacctgattc aggaaggcaa catcggtctg atgaaagcgg ttgataaatt
    21841 cgaataccgc cgtggttaca agttctccac ctacgcaacc tggtggatcc gtcaggcgat
    21901 cacccgctct atcgcggatc aggcgcgcac catccgtatt ccggtgcata tgattgagac
    21961 tatcaacaaa ctcaaccgta tttctcgcca gatgctgcaa gagatgggcc gtgaaccaac
    22021 gccggaagaa ctggctgaac gtatgctgat gccggaagac aagatccgca aagtgctgaa
    22081 gatcgccaaa gagccaatct ccatggaaac gccgatcggt gatgatgaag attcgcatct
    22141 gggggatttc atcgaggata ccaccctcga gctgccgctg gattctgcga ccaccgaaag
    22201 cctgcgtgcg gcaacgcacg acgtgctggc tggcctgacc gcgcgtgaag cgaaagttct
    22261 gcgtatgcgt ttcggtatcg atatgaacac cgaccacacg ctggaagaag tgggtaaaca
    22321 gttcgacgtt acccgcgaac gtatccgtca gatcgaagcg aaggcgctgc gcaaactgcg
    22381 tcacccgagc cgttctgaag tgctgcgtag cttcctggac gattaatcgg taggccggat
    22441 caggtgttac gccgcacccg gcactagtcc ctccgcacaa acgccacccg aaggtggcat
    22501 aagggggtag cgcgcatcgc tatccccctt tatatatcaa atggttacga gttcaaagtg
    22561 gttcggaata gtgccattta gtccccgttt atgggcaaaa tctggacaac tccgcaaccg
    22621 gattgaacct taccgcgtcc tggagataat caggtgcaaa gtgcgcatag gtcattgtct
    22681 gctgaattgt gctgtgccct aaaatacgct gaagggtaat aatgttccct ccatttatca
    22741 tgaaatgagt tgcgaatgtg tgccgtaaaa catgcacaga ctgaccgact ggtaaatcag
    22801 gccgcacagt ccttattgca cttctgacta tcgcataact ggggttcatt aatcttcccg
    22861 tcgctttgac tttaatcaga gattcaagat ctttagctat aggaataacc cgccgcgatc
    22921 cattctttgt ctccatgaaa ataaccttcc caccaattat gtgttcacct ttaatattgg
    22981 caacttcacc ccagcgcccg cctgtagcca ggcttaacaa tacagcctta agattatcgc
    23041 cagataaaag tgcggttagc ctgggaatat catcatgtgc aagataagcc atttccggct
    23101 gattaattgt tagcttgcgt atctcgtgaa acgggttatc acccacaaac tcttcaacat
    23161 caatcagctt ttcaaagaaa ccgctcatca ttgcacaata acgatttacc gttgactgct
    23221 taattccgcg attcattaat tcaatgcgat atctgataat gttttttcgg gttaagtggt
    23281 cacttctgtt aataccaagc tcttgcatat cacgaagaac cgcttcaatt ctgacccgat
    23341 tcatcgcgcc ataacgatga ttcttaccgt ggtacaccca ccacagctcc agaagatcgc
    23401 ttaacgcttt acgttcaata ggtttttcga cccagtcctt atcgtgataa tgcgtcagaa
    23461 catggcgttc atacatcgcc gcttcaccac gggtaggaaa tcgccgccga aatcttcttc
    23521 cttcagatcc agcaggccgc acgtccacac agtatttgcc atcagcgagc ttcttaatcg
    23581 acataaggaa gccccccaat gaaaacatca tcttgatcac aattttgaga aataaaggcc
    23641 agatggaccg ttaaccagtc ttcttgccgg agtgggatga ttccgcttcg ctttgcccaa
    23701 tgtgtgcgag agccggtacc acctgcccgg cctttggttc gacctcatcg aacaaaaacc
    23761 agtcacggta tttagaaaat ctaggatgct tgaaaagctt gatggtgatc tcaaggccag
    23821 gaaggttacg accatactca taactggtca gagcagaata agaaattcca atcatatcag
    23881 cgaattcccg ctgagtgaga tcttcagatt tacgcatgac gcgaattttt tgaggaatat
    23941 ccatttgaca aactcctaca tatgtgtaga ttaacaccac atatgaacag caaacaaaat
    24001 gttctaagtg gttccaaata gtgccaatta gaaaaagcac caatggcgga ggatatcaga
    24061 tgcaagaaag cactcaaaac gcgccggagt cactttgtgg cacacgatgc gatcaccagt
    24121 cagagatatc ggaagcagaa cacctgaaga ccggccgcaa agcccctgaa cgccaggaaa
    24181 tccggctttc acaaaaccct tctcatctcc tgtcaaaaga agggtttgcg atgtatatcg
    24241 gcaagaccgt tgacgccatc gtgagcatgg caaaagcagg gaaattaccc gctgtgtaca
    24301 tggcggatcc cctcaagcct ggcggaaacg ccgaacttta catcaaccgg aaagcatggg
    24361 atgaagcctg cgatcgactc actgaaaacg caccgcaaga atggcacgaa tgggaaaacc
    24421 gacttttttt atttaagcca gcagctggtt cacggaataa acgcatcagt aaaaaagctg
    24481 cgtgatatag aactggcaat cattgggagg acaggatgat taagaaatac agcgaacacg
    24541 ggagcaaggc tggcggagca atcgccattg acgaggattg cctgataaca ggagacccct
    24601 ggctggcaac agaaagggat ttagaaggca aaacattttt tgtcagaaca ataaagcaca
    24661 acgataagat cgtgggcgag attgtttatt caaccaccac gcttattcct gagaactatc
    24721 tggatatttc ttttgaagct gtaagcattc aggggtgcaa cgcatatctg aattacctga
    24781 agaacctgga agaaataaag cttgatcaca tctggagcac cattaaggat gaaattatta
    24841 ccctgcaaag aaaaggcctg aaccctgatt ttagtaaatt gccaatggtc atcgaaacca
    24901 tgaaagaacc aaaacgtttc cacgccctcc ttgactggaa tttgccagcc agagagggaa
    24961 tagacaagtt ggtacgacaa catctgagga caacgctttt gagttttgat gaatacctgc
    25021 aagttcagga ggttattaca ccaaactagc ccagtactaa tcctcattta atttaaaatg
    25081 gcaatcattg gaggggcaag atgattaaac acacggaaat cagacaaaga ataaaaataa
    25141 gcgataacgc ttcagtagta atctgtaatc agaacttaga tgccattgtt cgggctggca
    25201 acgatttgta tttccactac ccgacccatt gcatcgttgc gacatttgaa acagaagagt
    25261 ccttaaaaaa tgcaatggag cagacaggat ttcagaaaga aacagtaata agaaaagaca
    25321 actggacata aatgccagta caccgttatt ccaactctct taacttaaac ggggttttcc
    25381 gatatgacac agcgacgcag cttccgcact ggcacagaac gccacgcagc ccgtttctca
    25441 accagtgcat cacgcagcaa cacgcactac agtctgcagg aaacgcacgc cacacctgac
    25501 gggcatctgg ttaaacaaac cggtaaacac acctggctga ttaatgatgc cggaattgtc
    25561 atacacagag gcgcacgcaa cccgattacc ggaaatacct gttactccct gactcgcgga
    25621 gatgagcagt tcgggcagga tttcaccctg cacgaagcat taagaacggc agaccggtta
    25681 atcagcggtc gcagttttca gcacccggaa catcgccgct gataaataaa aggattcagc
    25741 catgatcgaa gcctacaaaa tacttattaa taatgcgttg cagcgttatc acttcgaact
    25801 cgcaaaccgg ggtgtaaccc gggacgaagg acttcgtaac tcactgcggg gcgcaattat
    25861 ggggctgtat aactgcgcac tgggcagcga tgaccgcgaa gcactggaag aattaaaagt
    25921 tattgtggcc gaactgtttg aaggtgaaat cgttgagcct tatttcacag aggtagcagc
    25981 atgagcgtat ttctttcatg ggtgattctg attatttccg tgcttatggc gattggcatt
    26041 atgcgaatta tcgaagcgct gaaaaatatc gaatctttat ttactggcga agaacgccgt
    26101 aaataaataa ccaaccggac agaaaacatt aaaaccatcc gcatctgcgg agggcttcac
    26161 tcaatccaga aacggagaaa cagctatgag caccaataca cctacagaaa taaacaaact
    26221 atttaccgac ccggcgcata ttgaattgac gcaaaaaacg ctgactgaaa taaccaatgt
    26281 actggatgaa agaatatcag aaatagataa agacaagcat tttgccacct acatggccct
    26341 gcaaatgcaa agcatggcaa tggtgacagc aaaacagacc atcagtgaac tctatatgaa
    26401 aaacctgcgc ctcgagcgcg aactggcaga actccgggca cagtccggcc agttttcagc
    26461 gtgaggggca ggtcgatgaa cagcatattt accgccacca tgctgacgcg tttcaccgat
    26521 gccgtgggcc acgagttcat ggtggaaagt catttgatca caacgaccac gccgtgcccg
    26581 tcagacgctg attatctcta catccatctg gctgacggca cacaaatcac cgcgattgcc
    26641 agtaccgttc gcgaagtaac ggccatcaga ggggcatgga aatcagaaac acaagcccac
    26701 ggagagctgc ggccatgatc agcaagtaca ccaccaacgc acgtaaaccc ggtaaagacg
    26761 cacgccacca catcgccgta ctccgcaatc tgttactgga ggaaatcgac gacttcacgg
    26821 caacgctgag cggtccgggc gcaccagtaa caccaggtga gatccaccgg gaccttaaac
    26881 gccgtattga aaacgccatt gattacaccc tgaacccgac cgaataaccg gaacagtaac
    26941 ggaggttatc gcgatgagac ttacacctat gggaataccg ggcaaagacc cggcacatgt
    27001 gaaggcgtgg acacagcagg aagatcggtt actcatctcg ctttatcagg aacacaacac
    27061 acatcagatg gctgaacgaa ttcagcgaac ccgtaaagca gttatgtacc ggattcgttt
    27121 tttacgtaat cgtgggctga ttggccgcaa gagaaaagca ccactgagta cagaagccat
    27181 agcctttctt attaaaaacc gtcatgtaaa aaccgcacag gaaatggctc tggaagtggg
    27241 atgcagtgag tgcacagtga ggtacacact gcgtaaacgg ggttacaacc tgaaaaaatg
    27301 cggtgagaac caccattgcg ccagatacag cgatcatctg gttgagctgg tcacggagct
    27361 acgcgacata cacaacatga cgttctgcat gatcgctgaa cacatcaaca gcacgataca
    27421 gatgcacatt aacgacgatg cggcgtttca tctctaccac cgccgcaccg caactgacgc
    27481 agtgctctgt gaactgttac cgggctgagt gatcatcatg cacacacaaa aaaaccgctt
    27541 gccagcgttc gcctggtcgg gttacagttt tcccgcacct cataaaacgg gtgccgggat
    27601 taccaccccg ccgactacaa gagcgcacaa ccgcgccagc ggtttttttg tgcgtactgt
    27661 atcgccacgt ctttttcgcg tcagaattat ggcggggcgt acggggccga cttcggtcgg
    27721 gccgggatct cttgtagccg gttgtggtaa ccctgtacgt ctcgccaccc tgagattacc
    27781 gcctcgggtt ggtgagtttt cagaacttac tacaagaggc caaacaccat ggcaaaccgc
    27841 aaacaacagc gcgcccgcgc tgagcgtctt cacatccgca ctgaaattaa ccgcagactc
    27901 catcgggctg catctgtcac cttcatcatg cagtcaaacg cattacatcg acttaacgat
    27961 ggtatcgctg ccgggtatta cgcggcagtc ttcagctatc tggccgaaga cctggcagaa
    28021 ctgcaggacc ttatcaacca gcaacaccac cactaaacaa catcctgaag ccatcccgca
    28081 cccgtgcgcg ggaggcttcc gcacgtctgt taccggaggt tctcatgaag agcgttaagg
    28141 gactttcaga tattcaggat ttttttaact ggatgtgggc agtggatccc cggctggctt
    28201 cccgcctgaa cgactggcac gactattacc gccgcgcctg taaacaggcc gccagacacg
    28261 tcaccacata cggggatttc tccttcaccg ttgacggccg ttaccgcgtg gacgtgaaag
    28321 gtgatggttt tggtttattt cacatcaacg gaaccgacag caccgccatc agttggtcgt
    28381 cgtcagagat gacgcttgtt ctggaaatga caaaccacag cgtggccgtg aaaggtatat
    28441 acaccgcaga agactttatc ggtgaatacc agcgccttct cgtgctgtac cgcgcggcgt
    28501 ttcaggcggc gactgccggg gagtgcgacg catgagcgac cataatgcac aactggcgta
    28561 cccgtggaac gccccgctac cggttatcga tcctgaaacg ttcggcagag cgcagccaac
    28621 cgcattacgc caggccattc agagctacat caacgaggat attcgcactg acgccagact
    28681 ggatgaagaa accgtcgcct tcctgacaac cacagacgaa gggaagcgaa ttaattcgca
    28741 tctttaccac gacgaagagc gtcggatcag gctggaaaaa ctggcgcggc acaaacacga
    28801 gaatccgcca acactggtat ctgaagccat ggcagagctg cgcgccctgc cgccgtttct
    28861 tgccggtccg ctcatccgtg acctggcgcg cctgagccgc aaacaggaga gcgcacgccg
    28921 ggaagggctt aagaataaca gcgcatatgc ggcggataac ttcgttcgtc acgggctgcg
    28981 tgcccgcctg aaacgtatcg cccgtgtgaa tgaacgcttt gccaccccgg cctttaaggc
    29041 caccgccgcg cgcgaacggc tggatgaact gcttatgctg ccacagctga gccgtgacga
    29101 aatccagcgc cttgccaccc tgaccgccac cacgttttcc accgcactgg agcgcgcatg
    29161 tgatgaagtc atcgaaaaga ccggcagggg tgacgatgac ctgctcacct ggttactgac
    29221 ctaccagcca ctggcacaca tggcgttaaa actgggtatc acgccaccct gctggccgtc
    29281 gctggaaatc aggcccgacc gccgcaccga ccccgacccg gaacgggtgc cgggtgcggt
    29341 catgcgcctt tcctgtgcga catggtggaa cagtcagtta agacacctcc gggacatctg
    29401 gcgtgaagaa ctgttacgcg ccgcaggtct ggtgtcacgt aagacatcgg tatatatcag
    29461 ccatgaagcg ttactggact ggcgcgaaaa acgccagcgc acccgcgatt ttctgaaggc
    29521 ctgggacatc gagaacgaag agggagaacg gttcagcctt gaggatatct actgggcagg
    29581 tctctctaat ccgcgtaacc gccgtaacga gatgatgacc tgcgcccggg gaatggagca
    29641 ggtcgccgaa gcacgcggcg acgtggcgtt tttcgtcacc gtcacagccc cttcccgttt
    29701 tcacagcgtg aacgatgacg gcagcctgaa cccgaaatac tgcggagcaa cggtacgcga
    29761 tgccagtgat tatctggttt accgcttttt cgccgccgtc cgcagggcca tcgacaaggc
    29821 cgggctgggc tggtacggcg tgcgcaccgt ggagccccac cacgacggta caccgcactg
    29881 gcacatgctg gtattcacca gccaggaaa
//
LOCUS       DAZKJR010000054        28853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKJR010000054 DAZKJR010000000
VERSION     DAZKJR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.054"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(14..757)
                     /gene="yjgL"
                     /locus_tag="ACHOWS_002413"
                     /pseudo
     CDS             complement(14..757)
                     /gene="yjgL"
                     /locus_tag="ACHOWS_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
     gene            complement(880..1332)
                     /gene="tabA"
                     /locus_tag="ACHOWS_002414"
     CDS             complement(880..1332)
                     /gene="tabA"
                     /locus_tag="ACHOWS_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFQ0047972.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(1477..2070)
                     /gene="bdcR"
                     /locus_tag="ACHOWS_002415"
     CDS             complement(1477..2070)
                     /gene="bdcR"
                     /locus_tag="ACHOWS_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0047973.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILEE"
     gene            2141..2854
                     /gene="bdcA"
                     /locus_tag="ACHOWS_002416"
     CDS             2141..2854
                     /gene="bdcA"
                     /locus_tag="ACHOWS_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0047974.1"
                     /translation="MGAFTGKTVLILGGSCGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2986..3381
                     /gene="yjgH"
                     /locus_tag="ACHOWS_002417"
     CDS             2986..3381
                     /gene="yjgH"
                     /locus_tag="ACHOWS_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFQ0047975.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3662..3796
                     /gene="pyrL"
                     /locus_tag="ACHOWS_002418"
     CDS             3662..3796
                     /gene="pyrL"
                     /locus_tag="ACHOWS_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFQ0047976.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3800..4735
                     /gene="pyrB"
                     /locus_tag="ACHOWS_002419"
     CDS             3800..4735
                     /gene="pyrB"
                     /locus_tag="ACHOWS_002419"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFQ0047977.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4748..5209
                     /gene="pyrI"
                     /locus_tag="ACHOWS_002420"
     CDS             4748..5209
                     /gene="pyrI"
                     /locus_tag="ACHOWS_002420"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFQ0047978.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5282..5668
                     /gene="ridA"
                     /locus_tag="ACHOWS_002421"
     CDS             5282..5668
                     /gene="ridA"
                     /locus_tag="ACHOWS_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFQ0047979.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5874..8570)
                     /gene="mgtA"
                     /locus_tag="ACHOWS_002422"
     CDS             complement(5874..8570)
                     /gene="mgtA"
                     /locus_tag="ACHOWS_002422"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0047980.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGVYSRRYGWQ"
     gene            complement(8711..8764)
                     /gene="mgtL"
                     /locus_tag="ACHOWS_002423"
     CDS             complement(8711..8764)
                     /gene="mgtL"
                     /locus_tag="ACHOWS_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFQ0047981.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8949..9896
                     /gene="treR"
                     /locus_tag="ACHOWS_002424"
     CDS             8949..9896
                     /gene="treR"
                     /locus_tag="ACHOWS_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFQ0047982.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAYWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10015..11436
                     /gene="treB"
                     /locus_tag="ACHOWS_002425"
     CDS             10015..11436
                     /gene="treB"
                     /locus_tag="ACHOWS_002425"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFQ0047983.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11486..13141
                     /gene="treC"
                     /locus_tag="ACHOWS_002426"
     CDS             11486..13141
                     /gene="treC"
                     /locus_tag="ACHOWS_002426"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0047984.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKASPYRQFYIWRDGESETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRRLMTVGEMSSTSLEHCQRYATLTGSELSMTFN
                     FHHLKVDYPSGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13526..15664
                     /gene="nrdD"
                     /locus_tag="ACHOWS_002427"
     CDS             13526..15664
                     /gene="nrdD"
                     /locus_tag="ACHOWS_002427"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0047985.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15822..16286
                     /gene="nrdG"
                     /locus_tag="ACHOWS_002428"
     CDS             15822..16286
                     /gene="nrdG"
                     /locus_tag="ACHOWS_002428"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFQ0047986.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16331..16717)
                     /gene="cybC"
                     /locus_tag="ACHOWS_002429"
     CDS             complement(16331..16717)
                     /gene="cybC"
                     /locus_tag="ACHOWS_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFQ0047987.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16900..18252)
                     /gene="pmbA"
                     /locus_tag="ACHOWS_002430"
     CDS             complement(16900..18252)
                     /gene="pmbA"
                     /locus_tag="ACHOWS_002430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFQ0047988.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18346..18897
                     /gene="yjgA"
                     /locus_tag="ACHOWS_002431"
     CDS             18346..18897
                     /gene="yjgA"
                     /locus_tag="ACHOWS_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFQ0047989.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIEQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19055..20428)
                     /gene="mpl"
                     /locus_tag="ACHOWS_002432"
     CDS             complement(19055..20428)
                     /gene="mpl"
                     /locus_tag="ACHOWS_002432"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFQ0047990.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20604..21602
                     /gene="fbp"
                     /locus_tag="ACHOWS_002433"
     CDS             20604..21602
                     /gene="fbp"
                     /locus_tag="ACHOWS_002433"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFQ0047991.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21635..22630)
                     /gene="yjfF"
                     /locus_tag="ACHOWS_002434"
     CDS             complement(21635..22630)
                     /gene="yjfF"
                     /locus_tag="ACHOWS_002434"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFQ0047992.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22617..23639)
                     /gene="ytfT"
                     /locus_tag="ACHOWS_002435"
     CDS             complement(22617..23639)
                     /gene="ytfT"
                     /locus_tag="ACHOWS_002435"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFQ0047993.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(23653..25155)
                     /gene="ytfR"
                     /locus_tag="ACHOWS_002436"
     CDS             complement(23653..25155)
                     /gene="ytfR"
                     /locus_tag="ACHOWS_002436"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFQ0047994.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25295..26251)
                     /gene="ytfQ"
                     /locus_tag="ACHOWS_002437"
     CDS             complement(25295..26251)
                     /gene="ytfQ"
                     /locus_tag="ACHOWS_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFQ0047995.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26561..27091
                     /gene="ppa"
                     /locus_tag="ACHOWS_002438"
     CDS             26561..27091
                     /gene="ppa"
                     /locus_tag="ACHOWS_002438"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFQ0047996.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27464..27970)
                     /gene="mug"
                     /locus_tag="ACHOWS_002439"
     CDS             complement(27464..27970)
                     /gene="mug"
                     /locus_tag="ACHOWS_002439"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFQ0047997.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            28095..28170
                     /locus_tag="ACHOWS_002440"
     tRNA            28095..28170
                     /locus_tag="ACHOWS_002440"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28128..28130,aa:Ile,seq:cat)
     gene            complement(28270..>28853)
                     /locus_tag="ACHOWS_002441"
     CDS             complement(28270..>28853)
                     /locus_tag="ACHOWS_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFQ0047998.1"
                     /translation="NLDREIWRDLMQRSGMLSLMDAQARDTWYRSLEYDNFPEISEAN
                     ILSTFEQLHQNKNEVFERGVINVFRGLSWDYKTNSPCKFGSKIIVNNLVRWDRWGFHL
                     NSGQQADRLADLERMLHLFSGKPIPDNRENITIHLDDHIQSVQGKEDYEDEMFSIRYF
                     KKGSAHITFRKPELVDRLNDIIAKHYPGQLSAI"
BASE COUNT         7119 a         7660 c         7152 g         6922 t
ORIGIN      
        1 tattcaatgc ttattctaat aaatccgtaa tcgtagaatc aactgaacct ttacaactca
       61 cctgcgagtt atttaagtcc tgcgagttat ttaactcctg agaatcattt aaatcctgcg
      121 aattatttaa ctcctgcgaa ttatttaact cctgtatatt atttaacttc tgtaaattat
      181 ttaacttctg atgattattt aatagacgcg ctatttcctt gttagcttcg cgttggtaac
      241 acgccagaac cttgttagta atcaggtgta atgaactatc atcttttctt tcaaaagact
      301 tcccttcata acgattagtc gaaccatcag attgttgaaa tataatagcc gtatgtcctt
      361 gacgagtccc catagtagtg atagtggaaa gtgtaatttc tatttccacc ttagataaat
      421 ccgctcgttc cccttcagga ttattcatgt aaaacttcat tttttcattg ccatcagtat
      481 ctttagaata agatgaggac tcaatatgag ccatcatatt tctacccgct tcgttggagc
      541 gtttgctaaa atccacataa cccagggtaa atatctttgc caaaaactct aaaaatgctc
      601 caggagaaag aatattcctt ctgctatcct gccccccggc aatacgggca aaatcattca
      661 ttccaacacg ccaggtattt aaaacttcac ttttttgttt aaaattgtcg gctaatgtaa
      721 tgtgttgaga ataattcaaa tctgatattt tgctcatgac gaactccttg agttaataag
      781 aacgtctaag cattacaaat tttactcaca aaaaaatatc aaaaaacgct cttttcacaa
      841 actcgccgta aacctggagt tatttacggc gaaaagtcat cacgccatca gcatcttcac
      901 tactgctttg cgaacccggg ctggcgcgcc cactgcgcac agcggtttat gcacttcccc
      961 cggataaaac acaacaaaat caccttcatt aaggataact gttttctcat caacgccttc
     1021 cggcaaaaat gcgatgtctt tatcagctaa ccagtcggta tccggcgtac ctgcaggttg
     1081 tgtgctgaag gtcatgcctt cctgaccttt taacacaatc tgaatgtcga gatagcgggc
     1141 atggtactcc gcacggcgag cttcgtacgg ctcggtcata tcttccgaga taagataaaa
     1201 cagacgattg ccttcgatat cgtgcttgcc cttgggcgtt tctgccgtaa cgtttgcttt
     1261 gatatgctca atcgcctggc gtaactcttg gggtagccac ggctgaagat tatgaatatt
     1321 tccgatgatc atcttttcca ctccttaatt aaaacaacgt ttcatttcta tcgttatacg
     1381 aaaagatgat caattccgct agccttaacg cgtgaattat tgtcttatca caacttatcg
     1441 gcaggcgcgt tcccgccttt cgggtcaagg cccgcatcac tcctcgagaa tagtttttat
     1501 cgcttccccc gccagggcag ctgttgcaca gagttgttcg atggagtgcc cctcccgtgc
     1561 cttcgcagaa agccctgaca tcacggtact cataaaatca gtcacacatt gtgcgctttg
     1621 tggatgccgc ctggcgatat agtcataaat ggtcgtttct gcggcgtgat aatattgaat
     1681 ggcaatatca cgcgcttgtg gatcatgact atgaatacct tcaagaacca tacagccagc
     1741 gcagccgccg ttttggctat atctgcgcgc cgcttctttt aatacctcaa ccaggcactc
     1801 gcctaccgga cgatcatcac gaagaatatc ggcaagcgga atagcttccg taccgacata
     1861 ttcattgagt acacgactaa acaacccagc cttactgcca aaagccgcgt agaggctcgg
     1921 ggggttaata ccaagataat cagtaacttc agagacactt actgcatcga aacctttttg
     1981 gtgaaaaagc acttttgccg cagagactgc ctgctcagga gcgaaacgtc tggggcgacc
     2041 tggaacgcga gattgttttt tagtgaccat tacaaaactt gttgacagaa agttaaaaca
     2101 gttttgtaat gcatgttaca taataaatca aggagtcctt atgggcgctt ttaccggtaa
     2161 gacagttctc atcctcggtg gtagctgtgg aatcggtgcc gctatcgtac gtcgtttcgt
     2221 caccgatgga gccaatgtac gattcaccta tgcggggtcg aaagatgccg ctgaacgtct
     2281 ggcacaagag actggagcga cagcagtatt cacagatagt gctgacagag acgctgttat
     2341 tgatgtcgtt cgtaagagcg gcgcattgga tatcctggtg gtaaatgcag gtattggcgt
     2401 ctttggcgat gccctggaat taaatgccga cgatattgat cgccttttca aaatcaatat
     2461 tcatgctcct tatcatgcct ctgttgaagc cgcccggcag atgcccgaag gcgggcgcat
     2521 cttaatcatc ggctccgtga atggcgatcg tatgcctgtt gcaggcatgg ctgcttatgc
     2581 cgccagtaaa tctgccctgc aaggcatggc gcgcgggctg gcccgtgatt ttggaccgcg
     2641 agggatcacc atcaacgtcg tccagccagg accaattgat accgacgcta atcccgccaa
     2701 cgggccaatg cgcgatatgt tgcatagttt tatggccatc aaaagacatg ggcaaccgga
     2761 agaggtcgct ggtatggtcg catggttagc agggccagaa gccagttttg ttaccggcgc
     2821 gatgcatacc attgatggcg cgtttggcgc ataaccgact acgctcaatt aagcccagcc
     2881 attatccatg atgtctgggt tttgtttact aacgtcgtcc ccctaaaagc ggctcccggt
     2941 aaatataaat cttctgttca tgtcgtaaat cttgaggata accaaatggt agaaagaacc
     3001 gctgttttcc ctgctggccg acattcactg tatgctgagc atcgttattc tgcggctatt
     3061 cgttccggcg atttgctgtt tgtttccggg caagtaggaa gtcgagagga cggaacacca
     3121 gaacccgatt ttcagcaaca agtcagactg gcatttgata atttgcatgc gaccctggct
     3181 gctgcgggat gcacttttga cgatatcatt gatgttacga gctttcatac cgatccagaa
     3241 aaacaatttg aagacatcat gacggtcaaa aatgaaatat ttagcgcccc accttatcca
     3301 aactggacgg cggtgggtgt tacctggctg gcaggctttg attttgaaat taaagtgata
     3361 gcgcgcatcc ctgagctata agcaatagtg ttagccgttc gttttcacac tccgccctat
     3421 aagtcggatg aatggaataa aatgcatatc tgattgcgtg aaagtgaaaa agaaaaaagc
     3481 aggaaatgtc tgcaattatt gataccgaac gacagttctc ccccagaatc acatcaaata
     3541 aaaatgcatg tattttgact tttaattcaa ataaaccgtt tgcgctgaca aaatattgca
     3601 tcaaatgctt gcaccgcttc tgacgatgag tataatgccg gacaatttgc cgggaggatg
     3661 tatggttcag tgtgttcgac attttgtctt accgcgtctg aaaaaagacg ctggcctgcc
     3721 gtttttcttc ccgttgatca cccattccca gcccctcaat cgaggggctt ttttttgccc
     3781 aggcgtcagg agataaaaga tggctaatcc gctatatcag aaacatatca tttccataaa
     3841 cgaccttagt cgcgatgacc ttaatctggt gctggcgaca gcggcgaaac tgaaagcaaa
     3901 cccgcaacca gagctgttga agcacaaagt cattgccagc tgtttcttcg aagcctctac
     3961 ccgtacccgc ctctctttcg aaacttccat gcaccgcctg ggtgccagcg tggtgggctt
     4021 ctccgacagc gccaatacat cactgggtaa aaaaggcgaa acgctggccg ataccatttc
     4081 ggttatcagc acttacgtcg atgcgatagt gatgcgtcat ccgcaggaag gtgcggcgcg
     4141 cctggccacc gaattttctg gcaatgtacc ggtactgaat gccggtgatg gctccaacca
     4201 acatccgacg caaaccttgc tggacttatt caccattcag gaaacccagg ggcgtctgga
     4261 caatctccac gtcgcaatgg ttggtgacct gaaatatggc cgcaccgttc actccctgac
     4321 ccaggcgcta gcgaagttcg acggcaaccg tttttacttt atcgcgccgg atgcgctggc
     4381 aatgccgcaa tacatccttg atatgctcga tgaaaaaggg atcgcctgga gtctgcacag
     4441 ctctattgaa gaagtgatgg cggaagtaga catcctgtac atgacccgcg tgcaaaaaga
     4501 gcgtctggac ccgtccgagt acgccaacgt gaaagcgcag tttgttcttc gcgccagcga
     4561 tctccacaac gccaaagcca atatgaaagt gctgcatccg ctgccgcgtg ttgatgagat
     4621 tgcgacggat gtggataaaa cgcctcacgc ctggtacttc cagcaagcag gcaacgggat
     4681 tttcgctcgc caggcgttac tggcactggt tctgaatcgc gatctggtac tgtaagggga
     4741 aatagagatg acacacgata ataaattgca ggttgaagct attaaacgcg gcacggtaat
     4801 tgaccatatc cccgcccaga tcggttttaa gctgttgagt ctgttcaagc tgaccgaaac
     4861 ggatcagcgg atcaccattg gtctgaacct gccttctggc gagatgggcc gcaaagatct
     4921 gatcaaaatc gaaaatacct ttttgagtga agatcaggta gatcaactgg cattgtatgc
     4981 accgcaagcc acggttaacc gtatcgacaa ctatgaagtg gttggcaaat cgcgccccag
     5041 tctgccggag cgcatcgaca atgtgctggt ctgcccgaac agcaactgta tcagccatgc
     5101 cgaaccagtt tcttccagct ttgccgtgcg aaaacgcgcc aatgacatcg cgctcaaatg
     5161 caaatactgt gaaaaagagt tttcccataa tgtggtgctg gccaattaat tgcggttggt
     5221 aataaaagtc tggctcccta taatgagcca gactttttac cgctgtaata aaggagaaat
     5281 catgagcaaa actatcgcga cggaaaatgc accggcagct atcggtcctt acgtacaggg
     5341 cgttgatctg ggcaatatga tcatcacctc cggtcagatc ccggtaaatc cgaaaacggg
     5401 cgaagtaccg gcagacgtcg ctgcacaggc acgtcagtcg ctggataacg taaaagcgat
     5461 cgtcgaagcc gctggtctga aagtgggcga catcgttaaa accaccgtgt tcgtgaaaga
     5521 tctgaacgac ttcgcaaccg taaacgccac ttacgaagcg ttcttcaccg agcacaatgc
     5581 caccttcccg gcacgttctt gcgttgaagt tgcccgtctg ccgaaagacg tgaagattga
     5641 gatcgaagca atcgctgttc gtcgctaatc ttgatggaaa tccgggctat catgcccgga
     5701 ttaagtctga tgacaaaagc aaaatcgccc gatgcgctac gcttatcagg cctacgtgat
     5761 tcctgcaatt tattgaattt gtaggccgga ttgggcgttt acgccgcatc cggcatgaac
     5821 aaagctcact ttgtctgcaa tctgaatccg ggccaccatg cccagtttat tctttactgc
     5881 cagccgtaac gacggctata gacccctttc accaactggg ttaacgtcat ataccctgcc
     5941 agaatcgcaa ccagccacgg gaaatagctt aacggcagcg cctgcagctg cagataactg
     6001 gccagcggtg aaaacggcaa tgcgatcccg acaatcatca cgatcacggt catgatcatt
     6061 aacggccacg atgcacagct ctgaataaac ggtacacgac gggtacggat catatgcaca
     6121 atcagcgttt gcgacagtaa gcccaccaca aaccagcccg actggaacag cgtttgcgtt
     6181 tccggcgtgt tggcatggaa tacccaccac atcaggcaaa acgtcagaat atcgaagatc
     6241 gagctgatcg gtccgaagaa gatcataaag cgccccagat ccgccggatt ccaacgctgc
     6301 ggcttttgaa tttgctcgtc gtcgacgtta tcaaacggga tcgccacctg tgacacatcg
     6361 tacagcaagt tctgaataag caagtgtaac ggcaacatcg gcaggaaggg caagaaagca
     6421 ctcgccacca gcacgctgaa cacattaccg aagttagaac tcgccgtcat tttgatgtat
     6481 ttcagcatgt tggcgaaagt gcgacgtccc tcaataaccc cctcttccag caccatcagg
     6541 cttttttcca gcaggatgat atcagccgct tcacgggcaa tatctaccgc gccgtccaca
     6601 gaaatgccga tatccgcagc gcgtaaggcg ggcgcatcat taataccatc gcccataaag
     6661 ccaaccacat gcccttcgcg cttcagtaag gtcacaatgc gttctttatg catcggcgtc
     6721 aggcgggcaa acagcgtggt gcgctgtgcg agatttgcca gttcatcgtc agatagcgtt
     6781 tcaatatcac taccaatgac cacctctccc gcatccagcc ccacttcatg gcacactttc
     6841 gctgcaacta actcactgtc gccagtaagg atttttacgg taattccact cgcttttaat
     6901 gccttcagtg ccggagcggt tgtctctttt ggcggatcaa ggaaagcaat atatccttcg
     6961 aggatcaggt cggattcatc cgcccgctgg taatctcctt cacgcgctgg caggtatttc
     7021 gtcgccaccg caaccacgcg cagcccctga cgattcagcg tatcagtaac ccgcttaatc
     7081 ttacgcagca tgatgtcatc gagcggcaca atctcgccat tgtgacgcac ctgcgaacac
     7141 acattgagga tttcctgcaa tgcacctttg caaaccagct gatggtgttc ggtattttct
     7201 gccactacca ccgacatccg acgacgctcg aaatcaaacg gaatctcatc aattttttgc
     7261 caacggctgg ccagcgaacg cgctgactct tcatccgtac cttcgagcac cgcggtatca
     7321 agcaggtttt taagtccggt ctggtaatga ctgttcaacc acgcgctatg cagcacgcgt
     7381 tcgctggttt taccggagat atcggtatga ttctccagca caattttgtc ctgcgtcagg
     7441 gtgccggttt tatcagtgca cagaatatcc attgcgccaa agttctgaat ggcatccaga
     7501 tgtttgacga tcactttctg tttcgacagc tttactgcgc cgcgcgccag cgtcgaggta
     7561 acaatcatcg gcaacatttc tggcgttaag cctaccgcga ccgaaagcgc aaacagtgcc
     7621 gcttcccacc agtcgccttt ggtgtaaccg ttgataagca acaccaccgg agccatcacc
     7681 agcataaagc gaatcagcag catactgacg cggctaatcc cttgctgaaa ggcattcggc
     7741 tcgctttcct gctcactaac acgccccgcc agttgaccaa accaggtatt ggcccctgtg
     7801 gcaatcacca ttgcttgtgc cgtaccgctc accacggtgg tgcccataaa acacagggtg
     7861 tcgcactcca gcggattgct gtgctccggc tggcgagtag tagcggcttt ttccacgggc
     7921 agagactcac cggttaacga cgcctgagcg acgaacagat cccgcgcctg caagatacgt
     7981 aaatctgccg ggatcatatc ccctgccgcc agttttataa tatcgccggg caccagttgg
     8041 tcgatcggga tctccagcca gccattttcg cctttgtcgt taattacgcg cagcaccgtc
     8101 gcagtattgc tgaccatcgc tttcagggca tctgccgctt tagtggaacg tgcttcctga
     8161 ataaagttca gcaacgtaga aatagcgacc attagcgcga taacgcccgc ggcaaataaa
     8221 tcttccgtgg cgtaagaaat agcgccgaga atggtgagta aaatattaaa ggggttgcga
     8281 tagcagaccc ataaatgaac ccaccacggc gacggttgtt gtgcgggtaa tttattttca
     8341 ccatgttgtt cgcgggcaga ttccacttcc gcctgattta atccttccgg atgggtgtcg
     8401 aacgttttcc acaattcttc ttcgggcatc accgccattt tcaggcaatg cgcacttaag
     8461 gacggcggga ccgccgtatt cactgtctgc tgcgcgccag gcaatggatc gcgatgaacc
     8521 agacgggaag gtaaatggcg aatgaaccgg gtaaaaattt ctttaaacat aaggagtccc
     8581 tccgcactgt ctgaaacagt acagaaatgt cttcaggcgt ggcgaagcct tacctgaagg
     8641 taataaagct gagcagggac gttaaaacgt cacggcctta cgcatccggt aacgccgtga
     8701 aaagacgggc ttaccggaaa agttttaatc tccgtcgagg gagaggcgtg ggatcaggtt
     8761 ccatataacc tccggtaagt gaaaaaagcg ccgcaaataa taaggagaat ccaataatat
     8821 tcgtagcatt tatggcgata taatacctgc tggcaactaa accaacgata aaccagactt
     8881 tacccattgc tgaatgcaca ggtaaagtta ggctcaaata attaaacaac acgttatggg
     8941 acaacaggat gcaaaatcgg ctgaccatca aagacatcgc gcgcttaagc ggcgtgggga
     9001 aatctacggt ttcccgggtg ctgaataacg aaagcggcgt gagccagcgc acgcgcgagc
     9061 gtgttgaagc agtgatgaat cagcatggat tttccccttc ccgctctgcg cgcgctatgc
     9121 gtgggcaaag cgataaagtg gtcgccatca ttgttacccg tctggattcg ttgtcagaaa
     9181 atctcgccgt tcaaaccatg ctgccagcgt tctatgaaca aggttacgat ccaatcatga
     9241 tggaaagtca gttttcccca caattagttg ccgaacattt gggggtgctg aaacggcgta
     9301 atatcgacgg cgtagtgctg ttcggtttta ccggcataac agaagaaatg ttagcctact
     9361 ggcagtcatc gctggttctg ctggcgcgtg acgcaaaagg ctttgcttcg gtctgttatg
     9421 acgacgaagg ggcaatcaag atcctgatgc aacggctgta tgaccaggga catcgtaata
     9481 tcagttatct cggcgtgccg cacagtgacg tgacgaccgg taagcgacgt cacgaagcct
     9541 acctggcgtt ctgcaaagcg cataaattgc atcccgttgc cgctctgcca gggcttgcta
     9601 tgaagcaagg ctatgagaac gtagcaaaag tgattacgcc tgaaactacc gcgttactgt
     9661 gcgcaaccga cacgctggca cttggcgcaa gtaaatacct gcaagagcaa cgcatcgaca
     9721 ccttgcaact ggcgagcgtc ggtaatacgc cgttaatgaa attcctccat ccggagatcg
     9781 tcaccgtaga tcccggttac gccgaagctg gacgccaggc ggcctgccag ttgatcgcgc
     9841 aggtaaccgg gcgcagcgaa ccgcaacaaa tcatcattcc cgccaccctg tcctgatcgt
     9901 ttcctgaacg ataaattgtg atcttcgctg cgtttcggga acgttcccgt ttttaaattt
     9961 ttcagcgcaa tatattctgc agccaaccaa aaatgtcatc tgccatgggg ctttatgatg
    10021 agcaaaataa accaaacgga tatcgatcgg ttgattgaac tggtcggcgg gcgcggcaat
    10081 attgcgacgg tgagccactg tattactcgt ctgcgctttg tcctcaacca accggccaat
    10141 gccagaccga aagaaattga gcaactcccc atggtgaaag gctgtttcac caatgccggg
    10201 caatttcagg tggtgattgg caccaacgtg ggtgattact atcaagcact gatagcgtca
    10261 accggacagg cgcaggttga taaagagcag gtaaaaaaag ccgcccggca gaatatgaaa
    10321 tggcatgagc agttgatctc tcatttcgcg gagattttct tcccgttgct gcccgcgttg
    10381 attagcggcg gtttgatcct cggttttcgc aatgtgatcg gcgatttgcc catgagcaac
    10441 ggtcaaacgc tggcgcaaat gtacccttcc ctgcaaacga tctacgattt tctgtggttg
    10501 atcggtgaag cgatcttctt ctacctgccg gtcgggattt gctggtcagc ggtgaaaaaa
    10561 atgggcggca cgccgatcct tggtatcgtg cttggcgtaa cgctggtttc tccacagctg
    10621 atgaacgctt atctgctcgg gcagcagctg ccggaagtgt gggactttgg catgttcagc
    10681 atcgccaaag tgggctatca ggcgcaggtg atcccggcac tgttagccgg gctggcgctg
    10741 ggcgttattg aaactcgtct taagcgcatc gtgccggatt acctctatct ggtggtggtg
    10801 cccgtttgtt cgctgatcct cgcggtgttc ctcgcccatg cgctgattgg tccgtttggt
    10861 cgcatgattg gcgatggcgt tgcctttgcg gtacgtcacc tgatgaccgg cagctttgct
    10921 ccgattggcg cggcattgtt tggcttcctg tacgccccgc tggtgatcac cggcgtacat
    10981 cagaccacgc ttgctattga tttgcagatg attcaaagca tgggtggtac gccagtgtgg
    11041 ccgctgattg cgctgtcgaa tatcgctcag ggttccgccg tgataggcat tatcatttcc
    11101 agccgcaagc acaatgaacg cgagatctcc gtgcctgccg ctatctccgc ctggcttgga
    11161 gtcactgagc ctgctatgta cggcatcaac ctgaaatatc gcttcccgat gctgtgcgcg
    11221 atgattggtt ctggtctggc aggattgcta tgcggcctga acggcgttat ggcgaatggc
    11281 atcggcgtag gcggcctgcc gggaattctc tcgattcaac cgagctactg gcaggtgttt
    11341 gcgctggcaa tggctatcgc catcatcatc ccgattgtac tcacctcgtt tatctatcag
    11401 cggaaatacc gcctgggcac gctggacatt gtttaatttt cttcggggcg caattgcgcc
    11461 ccctcgcatt tgcaggaata acgtaatgac taatcttccc cactggtggc aaaacggcgt
    11521 tatctaccag atttatccaa agagttttca ggacaccacg ggtagcggca ccggcgattt
    11581 acgtggcgtt atccagcgcc tggactatct gcataaactg ggcgttgatg ccatctggct
    11641 aacccccttt tatgtctctc cccaggttga taacggttac gacgtagcga actatacggc
    11701 tattgatccc acctacggca cgctggacga ttttgacgaa ctggtgacac aggcaaaatc
    11761 gcgcgggatt cgtatcattc tcgatatggt gtttaaccat acctctaccc aacatgcctg
    11821 gtttcgcgag gcgctgaaca aagcaagccc ttaccgccag ttttatatct ggcgcgatgg
    11881 agaatcagaa acgccaccga acaactggcg ttcaaaattt ggcggtagtg cgtggcgctg
    11941 gcatgcggaa agcgaacagt actatttgca tctctttgca ccagaacagg cggatctcaa
    12001 ctgggagaat ccagcggtac gcgcagagct gaaaaaagtc tgtgagttct gggccgatcg
    12061 tggggtcgac gggttgcgcc tggatgtggt gaatctgatc tccaaagacc cgcgtttccc
    12121 tgatgacctg gatggcgacg ggcgtcgctt ctacaccgac gggccacgag cacacgagtt
    12181 tttgcacgag atgaaccgcg atgtgtttac gccacgcagg ttaatgaccg taggtgaaat
    12241 gtcctccacc agccttgagc attgccagcg atatgcgaca ctgacaggca gtgaattgtc
    12301 gatgaccttt aattttcatc acctgaaagt cgattatccc agtggtgaaa aatggacgct
    12361 ggctaaacct gactttgtgg cgttgaaaac attgttccgc cactggcaac aaggaatgca
    12421 caacgtggca tggaatgcct tgttctggtg taaccacgat cagccgcgca ttgtttctcg
    12481 ctttggtgat gaaggtgaat accgcgtgcc tgcggcaaaa atgctggcga tggtgctgca
    12541 tggcatgcag ggaacgccgt atatctacca gggcgaagag attggcatga ccaacccgca
    12601 tttcacgcgc attactgact atcgcgacgt ggagagcctc aatatgtttg ccgagctgcg
    12661 caacgatggt cgtgatgccg acgagttatt ggcaatcctc gccagtaaat cccgtgacaa
    12721 cagtcgcaca cccatgcaat ggagcaacgg cgataatgcc gggtttacgg ctggcgaacc
    12781 gtggattggc ctgggcgata actatcagga aatcaacgta gaagcagcgc tggccgatga
    12841 gtcctcggtg ttttacacct accaaaagtt aatcgcactg cgtaagcaag aagccgtcct
    12901 gacatggggc gattaccagg atctgctgcc aaacagccct gtattgtggt gctatcgccg
    12961 tgagtggaag gggcaaacct tactggtcat tgccaacctt agccgtgaga cccaaccctg
    13021 gcagccaggg aaaatgctcg gcaactggca gcttgtgatg cataactacg aagaagcctc
    13081 accacaaccc tgtgccatga ctttacggcc ttttgaggct gtctggtggt tacagaagta
    13141 agtatccgct aagccccggt aatactggtc attccggggt tttgcctttc agcatgttga
    13201 ttttataatc aaaaaattca ttaaaaaatt tacataacgc tgtagcgccc gtcatccgta
    13261 cgctctgctt tttactttga gctacatcaa aaaaagttca aacatccttg atgcaaagca
    13321 ctatatatag actttaaaat gcgtcccgac ccaatatgtt gtattaatcg actataattg
    13381 ctactacaac tccccacgaa aaaggtgcgg cgttgtggat aagcggatgg cgattgcgga
    13441 aagcgccgga aaacgaaacg aaaaaaccgg aaaacacctt tcccaatttc tgtggataac
    13501 ctgttcttaa aaatatggag cgatcatgac accgcatgtg atgaaacgag acggctgcaa
    13561 agtgccgttt aaatcagagc gcatcaaaga agcgattctg cgtgcagcta aagcagcgga
    13621 agtcgatgat gcagattatt gcgccactgt tgccgcggtt gtcagcgagc agatgcaggg
    13681 ccgcagccag gtggatatca acgagatcca gaccgccgtt gaaaaccagc ttatgtcggg
    13741 tccatacaaa caactggctc gtgcttacat cgagtaccgt cacgatcgcg acattgaacg
    13801 tgaaaaacgc ggtcgcctga atcaggagat ccgtggtctg gttgagcaga ccaacgcctc
    13861 gttgctcaac gaaaacgcca acaaagacag caaggtgatt ccaacccagc gcgacctgct
    13921 ggccgggatt gtggctaaac actatgcacg ccagcacctg ctgccgcgtg acgtggtgca
    13981 ggcacatgaa cgtggtgata ttcactatca cgatctcgat tactcaccgt tcttcccgat
    14041 gttcaactgc atgttgatcg acctgaaagg catgctgacc cagggcttta aaatggggaa
    14101 cgccgagatt gaaccgccga agtcgatctc tacggcaacc gcggtaactg cgcagattat
    14161 tgcccaggtc gccagccata tttatggcgg caccaccatt aaccgtatcg atgaagtgct
    14221 ggcaccgttt gtcaccgcca gctacaacaa acatcgcaag accgcagaag agtggaacat
    14281 cccggacgcc gaaggctacg ctaactctcg taccatcaaa gagtgctacg atgccttcca
    14341 gtcgctggag tacgaagtaa acaccctgca caccgccaac ggtcaaacgc cgtttgtaac
    14401 ctttggtttt ggtctgggca ccagctggga gtcgcgcctg attcaggaat ctatcctgcg
    14461 taaccgtatc gccggcctcg gtaaaaaccg taaaacggcg gtgttcccga aactggtgtt
    14521 tgcgattcgc gatggcctga accataaaaa aggcgatccg aactacgaca tcaaacagct
    14581 ggcgctggag tgcgcaagca agcgcatgta tccggatatc ctgaactacg atcaggtagt
    14641 gaaagtcacc ggttcgttta aaaccccgat gggctgccgc agcttcctcg gcgtgtggga
    14701 aaatgaaaac ggcgagcaga tccacgatgg tcgtaacaac ctcggcgtga tcagcctgaa
    14761 cctgccgcgt attgctctgg aggcaaaagg cgatgaagcc accttctgga agctgctgga
    14821 tgaacgtctg gtgctggcac gtaaggcgtt gatgacccgt atcgctcgtc tcgaaggcgt
    14881 gaaagcgcgc gtggccccga tcctctatat ggaaggtgct tgtggcgtgc gtctgaatgc
    14941 tgacgacgac gtttctgaaa tcttcaaaaa cggtcgtgcg tctatttcgc tgggttacat
    15001 cggcatccac gaaaccatta acgcgctgtt cggcggcgag catgtttacg acaacgagca
    15061 gctgcgcgcg aaaggtattg cgattgttga gcgtctgcgt caggcggtgg atcagtggaa
    15121 agaagaaacg ggttatggtt tcagtctcta cagcacgcca agtgaaaacc tgtgcgaccg
    15181 cttctgccgt ctcgataccg ctgagtttgg cgtggtgccg ggcgtgaccg ataaaggtta
    15241 ctacaccaac agtttccacc ttgatgtgga gaaaaaggtg aacccgtatg acaagatcga
    15301 ctttgaagcg ccttacccgc cgctggcgaa cggtggtttc atttgctacg gcgagtatcc
    15361 aaacattcag cacaacctga aggcgctgga agatgtctgg gattacagct atcagcatgt
    15421 accgtattac ggcaccaata cgccgatcga tgagtgctac gagtgtggct ttaccggtga
    15481 gttcgagtgc accagcaaag gcttcacttg tccgaaatgt ggtaaccatg acgcctcccg
    15541 tgtgtcggtg actcgccgcg tgtgcggata tttaggtagc ccggatgcac gtccatttaa
    15601 cgccggtaag caggaagaag ttaagcgccg cgttaaacat ttggggaatg ggcagatagg
    15661 ttaatcttca ctctgcgtta agcaaatgcc ggatgcggcg taaacgcctt atccggccta
    15721 catttggcaa cggctgtagg cctgataaga cgcgacagcg tcgcatcagg cgttgattac
    15781 tggatgcagc ctacacgcgg actgataagc caggcgaaat aatgaattat catcagtact
    15841 atcctgtcga catcgtcaac ggccccggca ctcgctgcac cctgtttgtc tccgggtgtg
    15901 ttcatgaatg ccccggttgc tataacaaaa gcacctggcg ggtaaattcc ggtcagccat
    15961 ttaccaaagc aatggaagac cagatcatta acgatctgaa tgacactcgc atcaaacgcc
    16021 agggtatttc cctctccggc ggcgatccgc tgcatccgca aaacgtgccg gatattctga
    16081 aactggtaca acgcatccgc gccgaatgtc cgggtaaaga catctgggtg tggacaggct
    16141 ataaactcga cgaactcaac gctgcgcaaa tgcaggttgt tgatttgatt aacgtgctgg
    16201 tcgacggcaa atttgtgcag gatttaaaag acccttccct gatctggcgc ggcagcagca
    16261 atcaggtggt gcatcatttg cgatgataag ttccctgtta cctgagtgca ggcaacaggg
    16321 aaataaggaa ttaacgatac ttctggtgat aggcgttgcg ggtcgttttc agttgctctg
    16381 cggcagcctg cgcttctttt actttacctt catttgccag cttcagcgcg tcgtcaatct
    16441 gaccgaccag aatatcgaaa ccgtggcgga agtctttcat ttccgggctg tccggtgatt
    16501 tgccttcgag cttcggcggc gtcgcttttt gcgcatccag cgctgcggcg cgcatcttcg
    16561 ttaacgcgtc tttgacttgc gccgcgttat cggctttttc gaccactttt aaattgtcgt
    16621 tgagggtttc catattgtct tcaagatcag cggcaaacga cgccgaactg aataccaacg
    16681 aggagactgc aagaatagct aacaggcttt tacgcattgc tcacttcctt ttttatttac
    16741 acttcatcct tcacactgcc tcatcgacgg ctacgctcat tcgcccgagt cacgtactgc
    16801 gagtacgctc cccggtcctc attcactttc cgtctcgatg cagtgcgaat gattttgtgt
    16861 aattttttag tttatagcgc ggcaggtcgc gccagttttt tactgcccgg cgattttcat
    16921 ctccggcaac agcacagaac cacactgtat attactgcgt gtttcaatat cgttaccgac
    16981 ggtgacaata ttgcgccaca tatcttttaa attacctgcg atggtgattt cgctaaccgg
    17041 atactgaatt tcgccattct ctacccagaa gcccgctgca ccacgggaat aatcaccggt
    17101 aatggcactc acgccctggc ccatcagctc cgtcaccacc agcccggtgc ccatctcttt
    17161 gagcatttgt tcgaagctta gcccttgtcc ggcaatccgc cagttgtgaa taccgcccgc
    17221 atgtccggtg cttttcagcc ccagtttccg cgccgaatag ctggtcagca gccactgagt
    17281 caggatgcca tctttaataa tatcgcgacg ctcggtgcgc acaccttcgc tgtcgaatgg
    17341 cgtagaggcc agccctttca gcagatgcgg atgctcttca atggtcagcc agtccggcag
    17401 aatttgttta cccagcgaat ccagcaggaa ggtagattta cggtaaaccg atccacccgc
    17461 tatcgcccca accagatgcc caaaaagccc ggttgccact tcattggcaa aaatgaccgg
    17521 cgctttcatg gtggagagtt tacgcggtga cagacgcgat aaagtgcggc gagcacagtc
    17581 ggccccgacc cactctggcg tttgcagatc gctcatcgca cgaccaatgg tgtaggcgta
    17641 atcacgctcc atatcgccat tttcttcggc aattacacag ctggagagcg aatgacgcgt
    17701 agagcagtaa ccctgcaaca tgccgtggct gttgccaaaa actttgacac cgtagtggct
    17761 gttaaagctg ccaccttcgg tattggtgat gcgtttgtcc gcctgcaatg ctgcctgttc
    17821 tgcgcgggcc gccagttcaa tggcttcatc cggggaaact tccgcagggt ggaacaagtc
    17881 gagatccggt gcgtcaaagg ccagcagctc tttgtctgcc acgccggcac agggatctgg
    17941 cgaggtataa cgggcaatat ctagcgccgc ctgtacggtg cgggcaatgg cctgcgggct
    18001 taaatcggta gacgatgcgc tacctttgcg gttctggtga taaacagtga tccccaacgc
    18061 gccatcgcta ttgaattcaa cattctccac ttcaccataa cgcgtgctta cgctaatgcc
    18121 ggtggtcttg ctgacggcaa cttctgcacc gtccgatttg cctgaggcca actccagcgc
    18181 agtcgaaact gcttcttcca gaatcttgcg ctgcgcttca acttgagaga ttactttcat
    18241 tgcaagtgcc ataatgtaga gagtttctct gaagtctaac agagaaccgt ttttcagtgc
    18301 gcatcttaac tggtaacatt agcctctttt tttaaggagc ctgagatgac taagcagccc
    18361 gaagactggc tcgacgacgt tcccggtgat gacatcgaag acgaagacga tgaaattatc
    18421 tgggtcagta aaagtgaaat taaacgcgat gccgaggagc taaaacgcct tggcgcggaa
    18481 attgttgatc tggggaaaaa cgcgctggat aagatcccgt tagatgcgga tctgcgcgcg
    18541 gctattgagc tcgcccagcg tattaagatg gaaggtcgcc gccgccagtt gcagctcatc
    18601 ggtaagatgc tgcgccagcg cgatgttgag cctattcgtc aggcgctgga taagctgaaa
    18661 aaccgccaca accagcaagt ggtgttgttc cataaactgg aaaacctgcg cgatcgtctg
    18721 atcgagcaag gtgatgacgc catcgctgaa gtattgaatc tgtggcccga tgccgatcgt
    18781 cagcaactgc gtactctgat ccgtaacgcg aagaaagaga aagaagggaa taagccgccg
    18841 aaatccgcgc gccagatttt ccagtatctg cgtgagttag cggaaaacga agggtaattt
    18901 tcgttgttga tgtgagtatg gtttgccgga tgcggtgcac ctgtaggcct gataagacgc
    18961 atcaagcgtc gcatcaggca ctatgctcaa atgccggatg cggcgtgaac gccttatccg
    19021 gcctacacac actctatcag gctgaggctg tgaattactg cgcggcttcc gctttcttcg
    19081 ctaacccatc cagcagtttc tgatggatcc caccaaaacc gccgttactc atcaccagaa
    19141 tgtgatcgcc cggctgagcg gttttcacca ccatatctgc cagcgtatcc acatcgccgc
    19201 tccagtgtgc aggctgaacg caggcttctg ccacttctgc cacctgccac ggaatatgcg
    19261 ccggttgcag caggaagact tcatcggcac gacctaatga aggtgccaga tcgtctttgc
    19321 agatccccat tttcatggtg tttgagcgcg gttccagcac ggcaataatg cgcgccgtac
    19381 caccaacttt gccacgcagc gccgcaagcg ttgccagaat cgccgtcggg tgatgggcaa
    19441 aatcgtcata taccgtcaca ccattcgctt caccacgcaa ctccagacgg cgacgagcat
    19501 taataaacga acccagcgcg ttagcggcat ctgccggcgc tacaccaaca tggcgagccg
    19561 ccgcaatcgc catcaggcca ttgtgcatat tatgttcgcc taccagcgac catttcactt
    19621 cgcccacttt ttcgccatcc agcaagactt cccattcgga agcatcggtg gtcagctttt
    19681 tcgcctgcca gtgaccttgc tcgcccacca gctcctgctc gctccagcag cccatcgcca
    19741 tggtctgttt caggttgatg tcattttccg gccagataat acggccctgc cccggaacga
    19801 tacgcaccag atggtggaac tgtttctgga tcgctttcag gtcgtcaaag atatcggcgt
    19861 gatcgaactc aaggttgttg aggatcagcg tacgcgggca gtaatggaca aatttagagc
    19921 gtttgtcgaa gaaggcgcag tcatactcat ccgcttcgat aacaaagaag tcgctttcgc
    19981 ccagacgcgc cgaaacctca aagttccccg gcacaccgcc gatcacaaat cccggtttgt
    20041 aaccgcactg ttccagaatc caggttgcca ttcccgcggt ggtggttttg ccatgtgtac
    20101 cggcaacggc cagcacccag cggtcgcgca gcacaaaatc gtgcagccac tgcggacctg
    20161 acatataagg gatgtttttt tccagtaccg cttccacaca cggatttcca cgggtcatgg
    20221 cgttaccaat aatcaccaga tccggctgtg gatcgagctg gctggcatcg taaccctgaa
    20281 tcagttcaat gccttgcttc tcaagtaagg tgctcatcgg cggatacaca ttggcgtccg
    20341 aacccgttac ttcatggcct aactggcgcg ccagcatcgc cagaccgccc ataaacgtgc
    20401 cacaaattcc taaaatatga atgcgcatac gtcactatcc ttttttaatc tggcgctcat
    20461 tttacgcata tgtcgggcaa gtgagaaacg catttcagga taatccgtaa tttgctggcg
    20521 cgattcacct gagcgcaaca ttgggattat ttgttaagat tgttgcggtc gctttactcc
    20581 ataaacattg cagggaaagt tttatgaaaa cgttaggtga atttattgtc gaaaagcagc
    20641 acgagttttc tcatgctacc ggtgagctca ctgctttgct gtcggcaata aaactgggcg
    20701 ccaagattat ccatcgcgat atcaacaaag caggactggt tgatatcctg ggtgccagcg
    20761 gtgctgagaa cgtgcagggc gaggttcagc agaaactcga cctgttcgct aatgagaaac
    20821 tgaaagccgc actgaaagca cgcgatatcg ttgcgggcat tgcctctgaa gaagaagatg
    20881 agattgtcgt ctttgaaggc tgtgaacacg caaaatacgt ggtgctgatg gacccactgg
    20941 atggctcgtc caacatcgat gttaacgtct ctgtcggtac cattttctcc atctaccgcc
    21001 gcgttacgcc tgttggcacg ccggtaacgg aagaagattt cctccagcct ggtaacaaac
    21061 aggttgcggc aggttacgtg gtatacggct cctctactat gctggtttac accaccggat
    21121 gcggtgttca cgcctttact tatgatcctt cgctgggtgt tttctgcctg tgccaggaac
    21181 ggatgcgctt cccggagaaa ggcaaaacct actccatcaa cgaaggaaac tacattaagt
    21241 ttccgaacgg ggtgaagaag tacattaaat tctgccagga agaagataaa tccaccaacc
    21301 gcccgtatac ctcacgttat atcggttcac tggtcgcgga tttccaccgt aacctgctga
    21361 aaggcggtat ttatctctac ccaagcaccg ccagccaccc ggacggtaaa ctgcgtttac
    21421 tgtatgagtg caacccgatg gcattcctgg cggaacaagc aggcggtaaa gcgagcgatg
    21481 gcaaagagcg tattctggat atcatcccgg aaaccctgca ccagcgccgt tcattctttg
    21541 tcggcaacga ccatatggtt gaagatgtcg aacgctttat ccgtgagttc ccggacgcgt
    21601 aatctcctgc gcctctccac acggagaggc gtttttatcg ctgcgcaatg ttgactcttg
    21661 tcacaggcga actctgacga ttctcccaca gcaccgttaa tccacgctgt aatgctataa
    21721 aaataaacaa caaaataccg atggcgattt tcgtccacca tgagctcagc gtgccatcaa
    21781 agtttatgta agtctgaatc agtccctgaa tcgccacgcc aaaaagcgtc cctaataccg
    21841 ttccaacgcc accactcaaa agcgtaccgc caattaccac tgaggcgata gcatccagtt
    21901 ccacacctac gcccgccagc gcatatccgg cctgggtata aatcgagaag acaatccccg
    21961 ccagtgtcgc cagcccggta gagagcatat aaatgcgaat agtggtgctg cgagtggaaa
    22021 tccccatcag gttcgccgac gttgcgttgc cgccaatggc gtacacctga ttaccaaaac
    22081 gcgtacgatg cgcgaggaat atgccgataa ccaccaccgc cagcatcagc agccccatcg
    22141 cacttaagcg accgccgcca gggattttcc acgcaaggct tgagagcgtg tcataaacag
    22201 gatggtttat cgggatcgac tcttccgaaa cgagatagct gacgccacgc aaaaagaaca
    22261 ttcctgccaa agtaatgata aacgccggga tcttcagggc gtcaatcaga agccccataa
    22321 atgcgccgaa ggcacagccc atcaccaaca ccagcggaaa cgccagcagc ggcgagaggc
    22381 cgaaatcgcc aatcactttt gccagaaaca cgccggtaaa ggcgatcacc gaaccgacgg
    22441 agagatcgat cccaccggag aggatcacaa aggtcatgcc aacggcgatt atcccaagaa
    22501 aggcattatc ggtcaggata ttgcagatca ctcttgtgga agcaaaaccg ggaaactggg
    22561 tcagacagta gatataaccc aacacaaaga cgccgatggt gatcatcagt ggcaaattac
    22621 gttttatcac ggttacgtac ccctttaatc agactgataa agcgttgcga ctggacaatc
    22681 agcacgcaaa gcaccaccac cgctttcacc acctggttca tctccggcgg aaatccagaa
    22741 agcaaaattc cggtgttcat tccctgaata atcagcgccc ccaccaccga aagcagcagg
    22801 ttaaagcgcc cgcccatcag cgatccgccg ccaatcacca ccgcgagaat ggcgtccagc
    22861 tccagccata acccggcgtt attggcatcg gcaccgcgaa tatccgccgc cacgataatg
    22921 cccgcaatcg ccgcacatag cccgctcaac acgtaggtga gcatgacgat gattcgcgtg
    22981 tttaccccgg cattttttgc cgcccgaata ttgataccaa cggcttcgat aaacatcccc
    23041 agtgccgttt tgcgggtcaa cagccagaac aggagaagcg tcagcaccgc aataatgact
    23101 ggcgttggca ggaacaacag cgatccactg ccgaaccagg agagatccgg tgagttaaat
    23161 gtgacgatct gtccggaggt gatcagttgc gccacaccgc gcccggcgac catcaggatc
    23221 agggtggcga caaacggctg aattttgagg atcgctacca gtatgccgtt ccacaatcct
    23281 gccaggatgc cagtgcccag ggcgcttaac aaaacaatcg gcaggctgaa tcccgcgacc
    23341 gtcatcgcag ccgttgtggc tccggcgata gccatcaccg cccctacgga gagatcaatc
    23401 ccaccggtgg cgataaccag cgtcatgcca atcgccagta gcgcaacggg ggccgcacgg
    23461 ttaagaatgt cgatagggct accgaacaaa cgcccatcct gaagcaccac ctgccagaaa
    23521 tgcggggcca ccaggctatc gaccagcagc accagcaaca gtgccgccag ctgcggcatt
    23581 cctgttggcc agcgaaaacg ccttttcggc ggcgtagtgt ccgggagaga ttgaggcatc
    23641 acactattct ccttacgccg caatggcgtt catgatcgcc ggaacggaaa gctctgccag
    23701 cgggatctcc gccacctgtt tgcgatcgcg catgataatc acccggtcgg catagcccac
    23761 cagctcttcc agttcagagg agatcaccag cagcgccaga ccgtcagcgc acagcgtttc
    23821 aatcaggcgg atgatttcag cgtgggcacc aacatcaatg ccgcgggttg gctcgtcgag
    23881 gatcaaaaat tgcggacgag tcagtagcca acgtgaaagc aacacttttt gctgattgcc
    23941 gccagagaga aattcaatcg gttgttcagt tgaaggtgtg cgaatgccaa gctggcggat
    24001 aaagcgttcg gcaatctctt gctgttcttt gcgggaaatc ggtcgtagcc agccgcgctg
    24061 ggcctggaga gcgaggatga tattttcccg caccgaggcg gcggcgatga tgccatcggt
    24121 tttcctgtct tccgggcaga agccgatacc cagtaccgaa gcctgatgtg gcgatcgcag
    24181 ggtttgctgt ttgcctttga tcaacgccgt gccgctgtca gcaggtttga tcccgaagat
    24241 cacttcggcg gtttcggtac gtccggatcc cagcaagcct gccaggccaa cgatctcgcc
    24301 ggggcgtact tcgagatcaa acggtgcgat cgttcctttt ttgccgtaat ttttgaacgc
    24361 ggcaacgggt ttgtcgctca gcaatgttct cccggcacgc tgaagcgcgt gggtgtccag
    24421 ttcacgcccc agcatcattt ttaccagttc gatctgcggt agctcgcggg tttcccgaca
    24481 gcccacaaaa ctgccgttac gtaagacggt gatccgatcg ctaacctgat aaacctgatc
    24541 gagaaaatga gtgacgaaga tcaggctgac gccgcgatcg cgaagctgac gcatcaggcc
    24601 aaacagtaac tctacttcct gagtgtcgag actggcggtg ggttcatcga ggatcagcac
    24661 tttggcggag agatcgatag cccggcaaat ggcgacgatt tgctgcatcg cgactgaaaa
    24721 gcggttgagc ggttcgcgca cgtcgaggga gaaaccgtaa gatgccatca gttcggtggc
    24781 gcgcttttcc atctctttac ggcgtagaag gccgaaacgt ttgggttcgc gacctatgaa
    24841 tagattatca gcgaccgaca tattgggtag caggttgact tcctgataga cggtgccgat
    24901 gccgagttgt tgtgcgtggg cggtgttttt cggtgagata gcctggcctt ccagccagat
    24961 agtgccgcga tcggcgtggt atacaccggt taatgctttg attagcgtcg attttcccgc
    25021 cccgttttca ccgagcagcg ccatgatttc gccacggcgc aggctgaaat caacgttgtc
    25081 taacgctttg acaccgggga aaaatttact taatccttcg gtgcggagga tttcctggtg
    25141 ttggtcggtc gtcatggttt cccccggtgt aaagcgtgct cccagtaggc atgataagac
    25201 gcggtaagcg tcgcatcagg cttcagcgca caaccgccgg atgcggcgcg agcgccttat
    25261 ccggcctacg aatcggcatc aggcatagca accctcaata ccccatattt ttcttcttct
    25321 ctaactcttc ttttgcggta tcaggcagat aaagggtgga tttggttaac gtcagctttt
    25381 caggcatggt gccgtctttt ttatatttct ccagcgcgtc gaaggcggga cctgccatat
    25441 tcggcgtcag ttcaacactg gcgttcgctt cgccatcaat catcgctttg tagatgtccg
    25501 gcacaccgtc gatagagcct gtcaggatat ctttgcccgg tttcaggccc gcttctttaa
    25561 ttgcctgaat tgcaccaatc accatgtcat cgttatgggc gtaaaccatg cagatgtttt
    25621 tgccgttgtt ttccgctttg ataaagctct ccatcacttc tttgcctttg ctgcgggtga
    25681 agtcacctga ctgcgagcgg ataattttga tatttggcgc attcttaatg gcttcggcaa
    25741 agcctttctt acggtcaatg gcgacgctgg ccccaacggt gccctgcagc tccaccacgt
    25801 tgcatggttt gccattcact tcttttacca gccagtcacc aatcaacttg ccttcgagga
    25861 tgttgtcggc ggtgacagtg gtcatataga gagatttgtc tttcacatcg atggaacggt
    25921 caagcaagaa gaccgggatt tcggcatctt tcgcctcttt taataccggc tcccaaccgg
    25981 tcgctaccac tggagcgata aagatcgcat ccaccccttg cgcgacgaag gaacgtaccg
    26041 ctttaatctg gttttcctgc ttttgctgac catcggcaat tttcaacgta attccgcgct
    26101 tttcggcttc acttttcgcc acattggttt ctgcggcgcg ccagccggat tccgacccga
    26161 cctgcgaaaa tccaacggtt aatggagcgg ccaacgccat agacgacatg gctgccgaga
    26221 ctgcagagac tacaagtaag cgtttccaca tagggttgtc ctcgtcgggg taattattat
    26281 tggtgaaaag atgttcgcga aaaaactata gacaattcgt tatgtaacgg attgcgttac
    26341 atcacacttc aaaaaagtaa ataaaacgtt aatcacaagt ttgtaatcgc tttcatctca
    26401 ctatgaaaaa tgcggctacg gttatggatt ttcctgctct gtataccgtc ttaaaactgg
    26461 cgaaaaagga aaatgaagac gaaaacaagc gaagacattc ggcgcgagtt ggctataata
    26521 ctcggcactt gtttgccaca tatttttaaa ggaaacagac atgagcttac tcaacgtccc
    26581 tgcgggtaaa gatctgccgg aagacatcta cgttgttatt gagatcccgg ctaacgcaga
    26641 tccaatcaaa tacgaaatcg acaaagagag cggcgcactg ttcgttgacc gcttcatgtc
    26701 caccgcgatg ttctatccgt gcaactacgg ttacatcaac cacactctgt ctctggacgg
    26761 tgacccggtt gacgtactgg tcccgactcc gtacccgctg cagccgggtt ctgtgatccg
    26821 ctgccgtccg gttggcgtac tgaaaatgac cgacgaagcc ggtgaagatg cgaaactgat
    26881 tgctgttccg cacaccaagc tgagcaaaga atacgatcac attaaagacg ttaacgatct
    26941 gccagagctg ctgaaagcgc aaatcgctca cttcttcgag cactacaaag acctcgaaaa
    27001 aggcaagtgg gttaaagttg aaggttggga aaacgcagaa gccgctaaag ctgaaatcgt
    27061 tgcttccttc gagcgcgcca agaataaata agttcttctg gcgcaataac cctgaacacc
    27121 gggcttcggt tagtaagggt ttttttatgc ccgcaattta taaactctct actccactat
    27181 tattattttc agcagttgaa aggcttgaac ggtaagaaca agctaaactc gaccaccatt
    27241 ttgctgttca tagccacttg ctggaagtta gccggcctca ctcatactca ccgttcaaag
    27301 cgtgtccccg ttttacccct gtacatttgt taacgccagc agagtttttt atgcgattgc
    27361 agatatggaa agcggcccgg agagaaaagc aaaagatggg aaagttacaa aaaaaatcgc
    27421 gagatacatc gcataaaaca acgccacctt ttcggtggcg tttttatcgc ccacgcacca
    27481 ccagtgcctg gtccagctcg cgatacgctt caaccagttt ctccagtgaa acgcgactta
    27541 aaccgctggg atttggcagc acccaaatct gcgtcgaacc aatggtgaga gtttgtttcc
    27601 cccactgtgc accgcgctgg ctgaatccct gttcatatgc ttgtttgccc agaatcgcca
    27661 acgcctgcgg ctgataatct tcaatttttt caatcagctt acgcccgcct gcgtgtagct
    27721 cctgctttga aacttcattg gcttgcaccg ttggacggtc taccagtttg gtgacgccac
    27781 aacgataatc cagcagatgc tgtgcctcct gcggcttcaa ctgacggtcg gtaaacccgg
    27841 cctgatatat caccttccag aagcgatttg ccggatgagc aaagggaaaa ccagtcccgg
    27901 cggatgaaag cccagggttg ataccgcaaa acacgacccg taaccctgga gccaaaatat
    27961 cctcaaccat atttactcct ttctaacatc accaggaaaa gtataacgga ttgaaaatac
    28021 attgtttata aaaacagcag gcgcgcggta atggctggat tgcgacacgg agttacttta
    28081 taatccgcta ccatggcccc ttagctcagt ggttagagca ggcgactcat aatcgcttgg
    28141 tcgttggttc aaacccaaca ggggccacca tatacaacaa agggttagct agtaaatcag
    28201 ctaacccttt ttcttttgcc tttgaattgg gtcaggtaat gggtcaggta agctttttct
    28261 ccatctgact cagatggctg atagttgccc cggataatgc ttcgcaatga tgtcatttag
    28321 ccgatcaacc agttctggtt ttctgaacgt gatatgcgcg gagcccttct taaagtatct
    28381 gatactgaac atctcatctt catagtcctc tttaccctga acagactgga tgtgatcatc
    28441 cagatgaatg gtgatgtttt cccggttgtc ggggatcggt ttgccgctga acaggtgtag
    28501 cattctttcc agatcggcaa gtcgatcggc ctgttgcccg ctgttcagat gaaatcccca
    28561 cctgtcccac ctcaccagat tgttgacgat aattttactg ccaaatttac agggtgagtt
    28621 ggttttgtaa tcccagctca gtcccctgaa gacgttgatc acccctcgct caaacacctc
    28681 attcttattc tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcac tgatttccgg
    28741 aaaattatcg tactccagtg accggtacca tgtatcacgg gcctgtgcat ccattaagga
    28801 aagcatccct gaacgttgca tcaggtcacg ccagatttct cgatcaagat tgc
//
LOCUS       DAZKJR010000055        28831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKJR010000055 DAZKJR010000000
VERSION     DAZKJR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.055"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(109..1362)
                     /gene="amiC"
                     /locus_tag="ACHOWS_002442"
     CDS             complement(109..1362)
                     /gene="amiC"
                     /locus_tag="ACHOWS_002442"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFQ0047999.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1594..2925
                     /gene="argA"
                     /locus_tag="ACHOWS_002443"
     CDS             1594..2925
                     /gene="argA"
                     /locus_tag="ACHOWS_002443"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFQ0048000.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3163..4989)
                     /gene="recD"
                     /locus_tag="ACHOWS_002444"
     CDS             complement(3163..4989)
                     /gene="recD"
                     /locus_tag="ACHOWS_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFQ0048001.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPSGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4989..8531)
                     /gene="recB"
                     /locus_tag="ACHOWS_002445"
     CDS             complement(4989..8531)
                     /gene="recB"
                     /locus_tag="ACHOWS_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFQ0048002.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKLLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALMLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVDEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDSLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGITLEEA"
     gene            complement(8524..11412)
                     /gene="ptrA"
                     /locus_tag="ACHOWS_002446"
     CDS             complement(8524..11412)
                     /gene="ptrA"
                     /locus_tag="ACHOWS_002446"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFQ0048003.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11588..14956)
                     /gene="recC"
                     /locus_tag="ACHOWS_002447"
     CDS             complement(11588..14956)
                     /gene="recC"
                     /locus_tag="ACHOWS_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFQ0048004.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWEIGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDADRLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIARRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14969..15292)
                     /locus_tag="ACHOWS_002448"
     CDS             complement(14969..15292)
                     /locus_tag="ACHOWS_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFQ0048005.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15277..15684)
                     /locus_tag="ACHOWS_002449"
     CDS             complement(15277..15684)
                     /locus_tag="ACHOWS_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFQ0048006.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15681..16244)
                     /locus_tag="ACHOWS_002450"
     CDS             complement(15681..16244)
                     /locus_tag="ACHOWS_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFQ0048007.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16235..16705)
                     /locus_tag="ACHOWS_002451"
     CDS             complement(16235..16705)
                     /locus_tag="ACHOWS_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFQ0048008.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16890..17684)
                     /gene="thyA"
                     /locus_tag="ACHOWS_002452"
     CDS             complement(16890..17684)
                     /gene="thyA"
                     /locus_tag="ACHOWS_002452"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0048009.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17691..18566)
                     /gene="lgt"
                     /locus_tag="ACHOWS_002453"
     CDS             complement(17691..18566)
                     /gene="lgt"
                     /locus_tag="ACHOWS_002453"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0048010.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18717..20963)
                     /gene="ptsP"
                     /locus_tag="ACHOWS_002454"
     CDS             complement(18717..20963)
                     /gene="ptsP"
                     /locus_tag="ACHOWS_002454"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0048011.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20976..21506)
                     /gene="rppH"
                     /locus_tag="ACHOWS_002455"
     CDS             complement(20976..21506)
                     /gene="rppH"
                     /locus_tag="ACHOWS_002455"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFQ0048012.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21859..22005)
                     /gene="ygdT"
                     /locus_tag="ACHOWS_002456"
     CDS             complement(21859..22005)
                     /gene="ygdT"
                     /locus_tag="ACHOWS_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFQ0048013.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22191..22880
                     /gene="mutH"
                     /locus_tag="ACHOWS_002457"
     CDS             22191..22880
                     /gene="mutH"
                     /locus_tag="ACHOWS_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFQ0048014.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22949..23662
                     /locus_tag="ACHOWS_002458"
     CDS             22949..23662
                     /locus_tag="ACHOWS_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFQ0048015.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23800..24018
                     /gene="ygdR"
                     /locus_tag="ACHOWS_002459"
     CDS             23800..24018
                     /gene="ygdR"
                     /locus_tag="ACHOWS_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFQ0048016.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24126..25166
                     /locus_tag="ACHOWS_002460"
     CDS             24126..25166
                     /locus_tag="ACHOWS_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFQ0048017.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25198..26391)
                     /gene="lplT"
                     /locus_tag="ACHOWS_002461"
     CDS             complement(25198..26391)
                     /gene="lplT"
                     /locus_tag="ACHOWS_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFQ0048018.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLSAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRY"
     gene            complement(26384..28543)
                     /gene="aas"
                     /locus_tag="ACHOWS_002462"
     CDS             complement(26384..28543)
                     /gene="aas"
                     /locus_tag="ACHOWS_002462"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFQ0048019.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
BASE COUNT         7020 a         7899 c         7215 g         6697 t
ORIGIN      
        1 ttcaagccga cattcaaata ttcgacatca gtcacaaaac cagcatctaa tattcatgaa
       61 aatgttatct attcaacgaa atataaattc attgaataaa taaaaacatc atccccttct
      121 cgccagcgtc gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac
      181 ttcctgctgg aaagtcgccg ttttcagttt acgttcttcc tcaacgttac tgataaacgc
      241 cgtttcgacc agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc
      301 aacttgattt ttatgcagct tgttgatttt accgagctta ttcagcaccg ctttaccaaa
      361 cttcaggctg tcggcaatgg tcagcgactg caccatatcg aacatggtgt ggtcgacata
      421 gcggtcaccg cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag
      481 atattttgcc gcagtactgg ttgcaccttt ggttgagagc gcaaacacag aggaaccgct
      541 cggctgacga ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt
      601 ctgggctttt gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta
      661 caccttcata ttgccctctt tctcgatcag agagcgcaga cggcgagcta tttgcaatac
      721 cacgtctttt tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc
      781 agggtcaagc ataatgacaa taggacgatc gcgccctgct ttacccggtt gtggaccact
      841 ttgtgctggc ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag
      901 cagcgggtcc tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt
      961 aaacccggcg accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa
     1021 aaccatacgt acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc
     1081 gtcagcgcgg atttgcgcag ccatcccctt gagtaccgag ttcaggttta catcttcgat
     1141 atccaccacc acgcgttcag gattactcaa cgcgaactgc ttatatttca gctgacgatt
     1201 tgattctacc gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg
     1261 gctgaccgca gccaggctga cctgacttac gctcaatagc cacatggcac ccgcgccttg
     1321 cagtaaacga cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag
     1381 aaatctaagc tgtgtaacaa gtaaacgacc aatttgaccg gaaactttag cgaaagacgc
     1441 ataatctgtc atctataaaa cggtaaacat tctttttata ttcacggtat tactgataaa
     1501 aaactcgctc ccgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca
     1561 ataatttcga ataatcatgc aaagaggtgt accgtggtaa aggaacgtaa aaccgagctg
     1621 gtcgagggat tccgccattc ggttccctat atcaataccc accggggaaa aacatttgtc
     1681 atcatgctcg gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc
     1741 gggttgttgc acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc
     1801 gacgcaaatc tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc
     1861 gacgccaaaa cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact
     1921 gctcgcctgt cgatgagtct caataacacg ccgctacagg gcgcgcatat caacgtcgtc
     1981 agtggcaatt ttattatcgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat
     2041 agcgggcgta tccggcggat tgatgaagac gcgatccatc gtcaactgga cagcggtgca
     2101 atagtgctaa tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg
     2161 gaagagattg ccactcaact ggccatcaaa ctgaaagctg aaaagatgat tggtttttgc
     2221 tcttcccagg gcgtcactaa tgacgacggt gatattgtct ccgaactttt ccctaacgaa
     2281 gcgcaagcac gggtagaagc ccaggaagag aaaggtgatt acaactccgg tacggtgcgc
     2341 tttttgcgtg gcgcagtgaa agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt
     2401 tatcaggaag atggcgcgct attgcaagag ttgttctcac gcgacggtat cggtacgcag
     2461 attgtgatgg aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt
     2521 ctggagttga ttcgcccgct ggagcagcaa ggtattctgg tacgccgttc tcgcgagcag
     2581 ctagagatgg aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc
     2641 gccgcgctct atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac
     2701 ccggattacc gcagctcatc aaggggcgag gttctgctgg aacgcattgc cgctcaggcg
     2761 aagcagagcg gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag
     2821 gaacgtggat ttaccccggt ggatattgat ttactccccg agagcaaaaa gcagttgtac
     2881 aactaccagc gtaaatccaa agtgttgatg gcggatttgg ggtaagtggt ggaaatcgcc
     2941 agatgcgaca tgcgcaatgc ctggaggcat gataaacacg ctagcatcgc atcaggtatt
     3001 gacgctcatc gtcggatgcg gcgtgaacgc cttatccgac ctacatcaaa tcaaaggtgt
     3061 tgatggcgga tttagggtaa gcgacggaaa tcgccagatg cgcatatgcg caatgcccgg
     3121 aggcatgaca ggacgcgcat gcgtcgcatc aggcaatcac gtttattccc gcgaactaaa
     3181 caatgccgcc agaccactgc gccgctcagt gcgcgtggca attgccgcac ttaaaatgcg
     3241 ctcatcggca tacagcgaca gacggcgacg cgcgcgggtc actgcggtgt aaaccagctc
     3301 tcgcgttact accggcgtgc gttgactcgg taaaatcaac gccgcatggt cgaactccga
     3361 tccctgcgat ttatgtaccg tcatcgccca cgtcgtttcg tgctctggca ggcgactcgg
     3421 ttgcacagac ttaatattgc cgtccggcat cgcaaaccaa acgcgcgtcc cctgcccgcg
     3481 atccagcgca ataccgatat cgccattaaa caacccaagc gcgctgtcat tacgggcaat
     3541 catcaccggt cggccttcgt accaacgaga gtgcggatta cgatgaattt tgcgcttctg
     3601 ttgcataaac tgctcaattc gctcattcag tccagccacg ccaaacggcc cttcccgcag
     3661 ggcgcacaaa agctggtact cattgaacgc ctgaatgatt aaatccggct cggcacgcgc
     3721 ttgcagcaga tccagataac gcccgtaacc cgcaagagcc tcctcaagca tcgcaatata
     3781 gtcttcgccg ctctgtaaga gccgtttttc gatatcagta aaatcctgct gaaaaacggt
     3841 tttcactgcc gttttatcac cacggttgat cgccgcagct aactgaccaa tgccagaatc
     3901 gctgccgaaa cgatagcttt tttgcagcag gcagagactg tcgcgcaaag atgccgcttc
     3961 tgtgccagtt cctgacggaa cgtgagttcc cgtcaggcgg ctcaactgcc cggcacgctc
     4021 ggcggtaaag cccgcgttgg cataagcgca gatatcgccc agcacagctc cagcctcaac
     4081 tgaggccagt tgatcgcgat caccgaggaa gatcactcgc gcatgatcgg gcaacgcatc
     4141 gatcaggcgc gacatcatgg ggagatcgat cattgacgct tcatccacca ccagcacatc
     4201 aagatgcagc gggttaccgg catgatgacg taaacgctgg ctacccggct gcgcacccag
     4261 caatcggtgc aaagtgctgg catcttccgg aatgcgtttc ttttgttcat cggtcagcgg
     4321 taactggcgc aaagccttgc cgagagattc ggttaagcgc gcggcagctt tacccgttgg
     4381 tgcagccaga cggatacggc agcgttcgcc gtctgccatt tgaattaacg ctgccagcaa
     4441 cttcgctacg gtggtcgttt taccggtgcc agggccgccg gaaatcaccg agatccgccg
     4501 cgtcagcgcc actgccgccg ccactttttg ccagttaatt tcatcgctta ctggaaaaag
     4561 tttgtccagg gtttgcgcca gtagagcttc atcaacctca atggcatgat tcacttcgtt
     4621 gaaaaagcgt gccactgtgc gctcgttaca ccacatgcga ttcaaataaa gacgatcacc
     4681 acagaggatc atcggcgttg gttcatctcc ccggctgacc gcttgtgaag ccagcaagca
     4741 ttcttcccaa ttttgtaact caccgatttc gctgacgcag gttgctaaca gcggatgtga
     4801 cgcctcgtta ttttccagtc gtgagagcgg taaacagaca tgcccttccc cggcgtcgtg
     4861 gcttaacagg gctgccgcga gggtgacggc aggatgttca tctcccgcca cggtcagggc
     4921 aaattgcaca tccagcgggc gtaactgttt gtgctccaca gcttccagta attgcttttg
     4981 caatttcatt acgcctcctc cagggtgata ccggcaaaca tctcatccat caggtcaatc
     5041 aacccggcgt tgggtcgggt cgcgtagatc ccttgttgcg gatgttcttt atcaacacca
     5101 cgcaggaaca gataaataac gccgccaaag tggcgctcat agtcgtaatc agcaatgcga
     5161 tggcgcagat aacggtgcag cgccagggta taaagctgat attgcagatc atagcggtgt
     5221 gcctgcattg ccgctgccat agcctgttgg gtgtaagcag aactgtcttc acccaaccag
     5281 ttggatttat agtcgagcag gtaataacgc ccttcgtggc ggaacaccag gtcaataaag
     5341 ccttttaaca tgccgcgtac ctgcatgaac tccagcggcg ggcagccagc ggatagcgag
     5401 tcaaactggc gaattagtgc atcaagctgg ctggcgatga gcggttcgct aatcggcagg
     5461 taaaactcca tctccacctg tttatcgcgg tcggaaagct gactcaggct aacacccgtt
     5521 tcattgagag gtgcctggag gacagccgtg atccactcgg tcaacaccgg ttcccactgc
     5581 gattcaaagc cgccgagttc cagtttttcc tgtacccagt tcgggtcaac cggctgggta
     5641 aaatcgaggt cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc gcgcgggaac
     5701 tgatgcggtg ttaacgtcgg ttcgtcaacg acgctgacca cgcctgcggc atcaacatcc
     5761 agccgtggca tcagatcctg agcgataccg tgaccacgct gctgcaaacc ggagtagctg
     5821 gtgacacgcc agttatcgcc gggcaatcgt tgtagcgtcc tcgcattcag ttctgcagtt
     5881 atagcatcat taacctgcca gggctggtta tcaccagttt gtgccgtttg ccaggcaata
     5941 tcatcatcgc ataacgcttc aatacaggtg cgaagccctg ccgcatcttg cggttcccct
     6001 ttttgcagca aacgcccgag cgcactttgg tggacgtcgg tgtcaccttt tttatcgcca
     6061 cgacggcgca ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg cgtcagcgcc
     6121 acgtaaagca aacgcagatc ttccgccaga cgttcggcct ccgcgagatc gacgctttct
     6181 ggcgcagcat taagatccag aacagcctca aacgagtggc gatcgtgata gaacgcctga
     6241 tcctggacgc ggaaattggt aataaatggc aaccagacca gcggatattc cagccctttt
     6301 gatttatgga tcgtgacaat ctgcaccaga tgtttatcac tttcgaggcg catttgttgg
     6361 ctggaggcat tactgtctgg ctcgaggata tgttgcgata accagcgtac cagcgcatgt
     6421 tcactttcca gctgcgttcc ggcttcttgt agcaattcgc tgatatgcaa gatatcggta
     6481 agacgccgct caccgcctgc tgttgccagc aagttttctg caatgttacg cgccgacatc
     6541 agcgcccgca gcatcggcat aacgccacgt ttgcgccaga tttgccgata accatcgaac
     6601 tcttcgacga ccgcatccca cgcatgttcg tcattattca gcgtttcaat atccagcgca
     6661 ttcagcccca tcattgacgt tgccagcgca ctgcgcaggg tgttctcacg ttcgggagtc
     6721 atcaccgcct gcaacagcca aagcatctcc tgcgcttcca gagtttcaaa aacactgtcg
     6781 cggttcgaaa ggtaaacgga agggatttcc agcagcatta aggcatcgcg cacctgggcg
     6841 gcctcctggc ggctgcgcac cagcacactg atgtccgaag cacgcaccgg acgcgcgtcg
     6901 tcgccgttca tcagcaacgc ttcgccccgc tgtccggctt gtagccagtc gcggatttgc
     6961 gcagcacata cctgcgccat ggtactttga tagtcgccaa cgccgcagct ttcgccttcc
     7021 atcagccaca ttttcatcgc aggctgcgtt tcacctttaa acacaaaacg taacgcctga
     7081 tttttgccgg cagatttcac aggaataaac ggtatttcac gaaacatgaa cgcgtcatca
     7141 gtctggctga aaagtttgtt aacgctgttc accattcccg gtgcggaacg ccagttggta
     7201 tctaaggtgt agtgagcatg aacttcgcta cgcgccttca tataagtgaa gatatccgca
     7261 ccccggaatg cataaatagc ctgtttcggg tcgccaatca gcaacaatgc ggtttccggc
     7321 tgatggtgcc agatacggcg aaaaattcga tactgctggg ggtcggtatc ctgaaattca
     7381 tcgatcattg ccaccgggaa tcgcgtacgg atcgccgctg ccaatacctc gccgctttca
     7441 ctacgcagcg cggaatcgag ccgacttaac atgtcatcaa aacccaattc gccacggcgg
     7501 cgtttttcac gcgctactgt ttcgcggatc tcagccaatg cgcgggtgat caccagatcg
     7561 cggatcgaca atggttctgc aagcagttga tcgatcgcct caaacagtgg atgtcgcggg
     7621 gtttcccccc cggctttcgt gcgatcttct aagaaacgct gagagaattt ttccagcgac
     7681 tccggcaact gatagctgtt ggtctcttct tctgcccagg cgctgatctt gtcgatccat
     7741 ttagcctgat tgctacggtt aaacttgcgt cgatcaatac cagaagattc gatcagcgca
     7801 tccagttcac ccactgcgtc gcgccactgc tgttttacgg catcaatacg cgccacaatt
     7861 tgcgcgtggc gggaagcaag cgtttcatca tcgggcggcg gtgctttgat aaccggtgct
     7921 tcgccttgca gataacgatt aatatcgcgc agcaacgcct gcggcccttt ccaggtttca
     7981 aagaccacct gggcaatttc acgcggcagc gggtagcagt ggcgacgcca gaaatcggcg
     8041 caggcctggt agcgtagcag agactcatct tcaatcagct gctgctcaaa cagcatgccg
     8101 gattcaaagg cattcaggtt gagcatgcgc tggcaaaaac cgtgaatagt aaagactgcc
     8161 gcttcatcca tctgccgttc ggctaacaac aaccactgcg cggcttgcgc tttatcgtcg
     8221 atctcctcca gcaggagttt gtacagtgga ttgtcggtgg tttcacgcag acaggcgatg
     8281 cgcaactcgt ggatattgct acggatacga ccgcgcaatt ctgccgtggc agcctcggta
     8341 aaggtgacca ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc agaaccgcct
     8401 agtccaagta acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc tgtgccggca
     8461 gaggcttcaa tcaggcgctc gccctgtaag ggcaagcgca aaggatctag tgtctcggcg
     8521 acatcactca ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc tgacgttctc
     8581 ccacactttc cagccttcag gatgcacata ttcggctttc ccgttctggc tgccggaaat
     8641 ctgcgacaga atagccatgc cttgcggctc gaccaccgcc tgatggaaga aatcagcaag
     8701 tttttgcggc gtcagcagtt ttatctgggc cacgatttta tcacgcgaat cgaagcgcat
     8761 attgccgcga tcgaaatctt tacttaactt cgatgcttct tcgccgagcg tttgcggtgc
     8821 ctgcagcatc tgggttatta ccgcctgctg gatttgcgca aactcctctg gcttcatcgc
     8881 ccgcaatttt gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca agaatgaagg
     8941 ctgtttatca ttgctttgca aaaggaagcc catgccccac tgacgcccca cgctcattgg
     9001 aaacgcaaac acggcatagc ccaactgttc ttcggtacgc aactgattgt agaaccacgg
     9061 ctgtacgatc tgccccaaca gagagctata ggctgagctg gtgtattcat cgtagccagt
     9121 cggtacaaat accgctgcca gtgcggagtc ggtgctgtta ccggcttttt caaagatgac
     9181 agattgtttt ttatcgacca cgacatcttt gttacggcac cactccgaac catcagcgcc
     9241 caactgtttt tgcacatcgc gtgccagcgt tgttgcctgg gcttcggtca tgttgccaat
     9301 aaccataaac tctggtcgag cccctgattt taaggcgtcg cgataggcca gcacctcttt
     9361 caaagtgatt gaaggcaaaa ttttgcgccg ttcatctcgc gagaagtacg gcacttgcga
     9421 gagcatctgc gcgggcataa tcgcctgctc gaacgctttg cccttttctg cggaatccat
     9481 catctggtta taccaggact tcgcctgctc aagctgatct tccgtagcgg tatagctaaa
     9541 gtacccctcg agcaatgcct ggaacagctg cggcagacgc tgggtgtaac cattagcatt
     9601 aaccataagg ccgttgttag cgttggtgga aaaactaatg ccaccaaccg acgcctggtt
     9661 gcttaactga tcaagcgcta gcccggcaag ataatcattg agcgcaaaca tcacctgatt
     9721 gcgggcgctg tccatggctt tcggattacg caaaatcagg ctgacatcag ctttgggttc
     9781 gctggaaaaa taacggcttg gcgcatagac cacacgcaga ttcgactcat caacaataag
     9841 ctctggatgg tcatatttct tctctgactt aatcagcgag aaatcgtcag gaatataggg
     9901 gttaagctct ggcaaagaga gcgcaatgtc ggcggctttt ttctgccagt cagcgaaagt
     9961 ttgtgcgcta attttatcga cctgatacgg cgcatcgaca aagtaagccg ttttgttgtg
    10021 cggctctttc gggctgatat accagatacg cgcattctgc ggcgtcatca tcgccaggcg
    10081 ttcttttact gctttagcgt cgtaccgatc ggcaatattg actgcatcca gcgtatgctc
    10141 aacaggaacg cgaatcatgg tatctgccag ccattcgacg taatccatat cacgggtgat
    10201 tgacggataa cgaaagtcga tatccagcac attcgccagt tcatcgaagt attgtttatc
    10261 aatccctttt tcgcgtaaca gattgaggta gctaaaaatg gccgccacaa cctgatcgcg
    10321 attcgccaga cctttatcgg ttaaagacgc agagatcgct aatacgccgc tgttgccgtt
    10381 gacgatagga tcggaattgg cgctaatgcc ctcaactaat ccctgctttt gcagccagtc
    10441 agaaagtgta cctgggctgc gattaccgat cagataggta atcaattcat ccgttttact
    10501 acggaacttc gctgaattgt tatcgatgcg aaactcaacg cgcagaactt tacgcggcag
    10561 cgccgggacg taatgaatga taataccttt ttgcgcgtcg gtgactaccg gcacggtgat
    10621 ttccggtttt ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg ccatttttgc
    10681 caactccggc agcggtttat tgctgtaaat aaccgccttc atcagattgg cagagtaata
    10741 tttctcgtga aaatctttca gcgcctgctg caccggattt ccgggtttgt cgcttaaagt
    10801 ttcgaggtta ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg tttctgcgct
    10861 gacctgtgcc atgcgcatcc cgtcacgcgt acgcgccatg gttaattcgg cattcactgc
    10921 attacgttca cgttcggcgt atttcttgtc gagcaagggt tctgcaatag cgtccgccag
    10981 gcggtctacc gcaccgggca aggcgtcgtt ctcaacttcc agatagaaag ccgtgcgata
    11041 cggtgccgtg ctggcattgt gactgccgcc gtgcattttg agatattcgg ccagactgtc
    11101 agcctgcggg tactttttcg accccatcag actcatatgt tcaaggtaat gtgccagccc
    11161 ctggtacgcc tcgggatctt ccagcgaccc aacgggcact accagcgccg agagcgattt
    11221 aaccgcctgc ggatcagaaa ccagcaagac caccataccg ttatccagac gtatagcctg
    11281 atactggcgg ttatctttat cacttttacg gatggtttcc tgaatcggct gccatcccgt
    11341 ttctgcctga cttaagggtg cccaaagggc aacaaacaac aataatgctt tgaaccaggt
    11401 gctgcggggc attcacggac ctcataatca actttatttt ctggccagat tcaacaggtt
    11461 aacgcaaatc atctgccaga atttaatctt gtgctgaacg agtcagccta tgtctatata
    11521 tccatcagtc cgtgactggt gcgcatcata aagcaagcag atatattgcg caatttttat
    11581 acagcactca tgactgatta aagcgaaaca gcggtaacag gaaacgttgc gactgttcaa
    11641 cgatagcctc cattgtctct ggtgttaatt gccgccagag ccgttgatac cagatatcat
    11701 caccttcgcc acgcaccatc atgttgcctt cgtaagcctg aaggaatttc gtacgggctt
    11761 tttgtaacgt ggaatcgtca tccagcatgg catcgttttg cgcgtcataa caggttttta
    11821 gccacgcgcc gccactttca ggtaacacca gcaatggcgc ggacattcct tcacgatacc
    11881 cctcaatcag ttgtgagagg taatgcaaag cctgttcggc tgcaagcggc ggaaaacgcc
    11941 actcgccgtc tttgcgtaga aaaaggcgac tttcaccatt accaccgctg gcacagtaga
    12001 caaggtgttc cagccaaagt tgcattccct gcgccacact taataaagag ggacgccagc
    12061 gcaacaggcc atccggctgc acctgcggca accagccagt tatctgcaca ccgttgcagg
    12121 cgagatcaat ttccatactc tgccccggct ggcgacgtgc aatgactctg tcggcaagct
    12181 gctgcatctc ctggcactgt gtttcccaga aaatttcacc aaaagcgcca tacggtaaat
    12241 cccctgccgc ccggaagcgg cggaataagc gatcggcatc atcctgctca accagtgcat
    12301 tcaataactg ctgattgatt tgataacggc taagcccttc cagaataaat ggctcggtgt
    12361 cggggatttc gctgtcttca gtacggaagt tcacctgcaa acgcatctgg aagaacgccc
    12421 gtaccggatg tgcccagaat cgttgtagcg tttccagcgg cacggtttcc ggtaaggtaa
    12481 acggcagcgg ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc gcaggtagcc
    12541 attcacgagc atagctttgt cgttcgcctg gctggtagtt ttgcggatca aacggcatcc
    12601 gggtatggag gcaagtaaga tgcgctttta cccttgcctc gctttcatca cagttgagcg
    12661 cttcatcgcc cggtagataa tggctttgcc cgatgtagtc gatcagttcc tgcaccagta
    12721 ccgacgggaa acgttcactg ttatcctgaa tagaacgccc gatatagctg atatagagtt
    12781 tttgctgcgc ggaaattaac gcttccagga acagatagcg gtcgtcatcg cgacggctac
    12841 ggtcgccacg cttcggtttc tggctcatca ggtcaaagcc caatggcgca agctggcgtg
    12901 gataaacgcc gtcgttcatt cccagcaggc aaaccacttt gaatgggatg gaacgcattg
    12961 gcatcagagt acaaatgtta accggtccgg cgagaaaacg ctggctgata cgttcctgat
    13021 ccaggcgctg tgccagttca tcacgcaata gtgacagcgg caccgcgtcg ccatactgcg
    13081 cacctaaacc ttcggcgata atcgcctgcc attgttgttc gatcagcgtc atagccgctt
    13141 cggtttccgc atccggcagg aagaaggcgt tgagcatatc gcgacaaacc ggcaaccact
    13201 cttccagcgg acgctcctgc gccagcccgc gacgccagat gtttagctgc attagcagtg
    13261 aagccagatg ccccaccagt tctgcaatta agccgctcga ttcatcataa ggtagaaccg
    13321 attgccactc gccctgcgcg ctctccatcg cgtagcccag caacatacgc gtcaggccaa
    13381 atcgccaggt gtgttgtccg gtggcgggga gttccagctc gcgaacgttg tcgtcatcta
    13441 tcccccaacg aatgccggat tcgttgaccc actggcgtaa ataacgcagc ccttcttcgg
    13501 tgatgtcaaa ccgcgccgcc agcaccggca catccagcag cgccagcaca tcctctgaca
    13561 caaagcgact gtcaggcagt gataacaggc tgataaacgc ttccaatacc ggatgtgact
    13621 gccgcgcccg acggtcggaa atggcgtaag gcaggtaacg atccgcaggt gcgctaccaa
    13681 acacagcctg aataaacgga ctgtagctgt cgatgtcggc caccatcacg atgatgtcgc
    13741 gcggagtcag tgtcgggtct tcctccagca tcgccagcag gcgatcgtgt aaaacttcaa
    13801 cttcacgctg cgggctatgg caaacgtgaa aggtgatact gctatccagt ggatcaagcg
    13861 ggcgtttgtt atcgctacgg gaaaactctt cgatgtttac accagcaacg gcgcggtttt
    13921 ccagttccag aatgtcagac tgaatgttat gcagcaggtt atctggcgta acatcgacaa
    13981 aagcgtccag ctcctggctg ctctccagat cagaaaggag ataaatgtag tcgcgcccaa
    14041 gcttgcccca tgaggccagc agcgggttgc cgacatcctg ttcaccatcg ctgttaaaga
    14101 gctgcccggc attttcgctg tcgcgaaata gcggtaattc tcgatcttca aaactgtgtc
    14161 ggcgctggcg agtcagtagt ttcgccagat aagcaggatc tttaatgtcg ccccagtaat
    14221 aacggcaggg gttggtaaac aggagatgga tttcaatatg tttacccagc gcctggagtg
    14281 cctggagata aacaggcggt aacgcggaaa taccgcatat aaagacgcgc gaaggtaacc
    14341 ccggcgggca ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag agattggcgc
    14401 ggtgccagcg cggttgcccg agttcatggg tatattccac caacgccttc cacaacggcg
    14461 cttgccaggc ctgtgcttct cccagccctt caaccaaatg tcctatttcc cactgtgcca
    14521 gccagtccgg acgatagacc agatactggt caaacaggtc cgccgctttt gaagaaagct
    14581 ggaacagttt tcgcttgtcg ctatcgtcag tcagataatg ccgcaacagg gtaaagtctt
    14641 cgcgctccaa cagttgcggc agcagagtca tcagtttcca gctcatgctc tgtttgttaa
    14701 aggcgctctc tttggggatt tccggtaaca cccggacgaa catatcccag ataaagctcg
    14761 ctggcagcgg aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg gtcatttgca
    14821 gccactgcgc cataccggta ctttgcacca gaatcatttc tggttcgaaa ggatcgtcca
    14881 gccgttcgcg ttcgacgata aactccatca acgcttccag cacgtccaga cgattggaat
    14941 ggtagaccct taacatagcg gctcctgact actgacgatt cgggcaatgc aggcgggtca
    15001 tctcgccttc tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca catcccgcct
    15061 gcgatgtctg cattcggttg acctgccagt tagcaggtgg cgaaatcgcg cgcagttgcg
    15121 tttgctgcca gccatgccgc cagagctgtt ggtactggtt tctgctggca agactgttca
    15181 ttaatgttcg ctggaaaccc gataacgcag tgacaatcat caccatcaac accatcgcca
    15241 acattacctc cggcagacta aagccttgtt gattcctcag ggaagctgac ataacgccct
    15301 ctctttcagc ggacaaaaat cgctccagcc gcgtggcgaa aaaacaatgt ttccatcgat
    15361 gacttcgcct gttcgccaca acgaaacgcc ttcataacca gcaatcaata aggcttcatt
    15421 atctgccagt aaacgcagac aaacccgggc accggtggca gcatacagta agcactgaac
    15481 tgcgggcagc gtctgccagg agtgcatttt tccccactcc agcgccgact gaacgatggc
    15541 ctggcggcgc aatgactgac tttccatgct cacgcgagaa gcaaaactgc gatcctgctg
    15601 actcattcct tgcaatagca agctacccaa aatcagcagc atcaggacca gagccagtga
    15661 cgaaacaccc ttttcgcggt tcacaggttg gatcctgtca cgctatagct ggcatccacc
    15721 acggtttgcg gatcagcctt gctggcagcg tgcatattaa ccgtcaacac cggcgagaag
    15781 ccgctgacat cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc cggattagtg
    15841 actttatccc agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc cagcacatgc
    15901 tccttcagac gaaatccaat ctggtcggac tcttttaccg gttcgcgatc ccagatacca
    15961 ttactgttcg catcccactg cacaatgaca cagtcaccct gtccgacaat ttccagccct
    16021 tcgcccgtac agctgccatg gcaataaccc gccctctgga gatgcttcgc gacggtaaat
    16081 acccgcagcc agatttcatc ttccagcgcc agcttacggg tgttcgttaa actttcacgc
    16141 tgtaacgcag gcagaaagcg tgcagccccc agcaacaata cgctactgat cgccatagca
    16201 atcaacactt ccagcagaga aaaaccttgc tcttttacag gcatccttct gtttctcctt
    16261 gctgacaaag ccggagtctt ccccacggcg aaaccactag ccaccactcg cccgttgagt
    16321 ttttgaagcg aatatgcccg gcccatgcgg tattgcgcag gccaaagaaa gcaagcgaag
    16381 gtgtcaggtc gctcatttcg acttcgggcc agcgcggcac aaagaccaat ggtgaactgc
    16441 catgacaggt attcgcccca gcaacggaac tcacaaggca ccataacgtc ccctccctga
    16501 taagactgat actgtggtcg cggttatgcc agttggcatc ttcacgtaaa tagagcaaat
    16561 agtcccgcgc ctggctggcg gtttgccaaa gccgttgcga ctgctgccag tattgccagc
    16621 cataaagccc acttgcgctt agcatgacca gaatcagcat cgcgaccagc gtttcaatca
    16681 gcgtataacc acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa aaaagacgag
    16741 ggccagtttc tatttcttcg gcacatcttc cggactattt acgccgttgc aggacgttgc
    16801 aaaatttcgg gaaggtgtct cgaagaattt aacggagggc aaaaaaaacc gacgcacact
    16861 ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aatgcccgga
    16921 tgtggatcgt atccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt
    16981 ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt
    17041 tgatccatat ggttgctgta gagatgcgtg tcgccaccgg tccagacaaa atcacccact
    17101 tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttgaac
    17161 ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct
    17221 gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct
    17281 acgttccacg ctgaaacaat aatgcggcgg gaatccgggt cgtttttcag ctggttcagt
    17341 acagtagtga tctggtcaat atgacgacca tccggcgttg gccaggcgcg ccactgttta
    17401 ccatacactg gcccgaggtc gccgttttca tcggcccatt cgtcccagat ggtgacattg
    17461 ttttcgtgta gataagcaat gttagtgtcg ccctgcagga accacagcag ttcgtggatg
    17521 atggaacgca ggtggcaacg tttagttgtc accagcggga atccatcctg caggttaaaa
    17581 cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg
    17641 ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg
    17701 tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc
    17761 gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccagt aaactgcgcg
    17821 tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtaaccaat caggaacaag
    17881 cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc
    17941 accacacctt ccagcagcag ctcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa
    18001 gtgtcgaaaa tggattgcca ctgcggattg gtttgcagca gcaaaatatc ttctgtacgg
    18061 gagccaggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg
    18121 ttaataaagt tgcccaggcg cccggcacca agaccaaacg gaatgagtgg ggcaataaaa
    18181 tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata
    18241 acgccaatca ggcctccgtg gaaggacatg ccgccgtccc atacacggaa aagatacagc
    18301 ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg tccaccgagg
    18361 aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccactg
    18421 cccggacgat tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc caccagatac
    18481 atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc
    18541 ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag
    18601 agaacagcga ttgaaatgca cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc
    18661 acaaggggac ctggctggtt gccggatacc gttaaaaaat atgtatatga tccgcgctat
    18721 aaccccccgc gaatcaaccc gcccatgccg cgacgctcca taaaggctgc aacctgatgg
    18781 cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcagca
    18841 aaatcaatgc gccgcagcag gtattttacg cgcgctacag aacgtccgtt catagacaga
    18901 tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg
    18961 cacaaacgga gatcgattcc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt
    19021 aacattgcag gatgaagact gtcataaatg ttcgccaccc gggtattgtt gcgatcaacg
    19081 gccaggatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc
    19141 agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg
    19201 ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt
    19261 gcttcgtcaa cttcatcgag gcttgtgacc atcggcaaca gaatattcag gttgcccgta
    19321 gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc
    19381 gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctaatcgg catgtaaggc
    19441 agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc attaaacatt
    19501 tgcagcatcc cctgatactg cgccacctgt tcttcttccg acggaaaacc actttgcagc
    19561 atgaatggga tttcagtgcg ataaagtcct atgccatcaa tacggctgcc cagtttttct
    19621 tcatgttccg ggcttaaacc ggcattgagc atgactttaa tgcgctcgcc gctttttaac
    19681 tgggcgggta aattgacgtc atcttccgcc agacggctaa gctcgatctc ttcactaatt
    19741 agccgctgat attcttgcag cagtaccggt tccggatcga ccagcaattc accgcgataa
    19801 ccatcgacga tcagcgtccg acgatgcagt accgaaggct gaatatccgc gcccatcacg
    19861 gtaggtatcc ccagcgcacg taccatgatc gcagcatggg agttggcagc gccatcgcgc
    19921 acgacaacac cgactaagcg atcctggggc agctcagcaa gcgttgtcgc tgacagttca
    19981 tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga
    20041 tgaaacagca atcgctgacc cagcgcacgt aaatcgccag cccgctcttt gagatagtta
    20101 tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct
    20161 gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt atccgaaagc
    20221 aggtgtgagt aaagatcgaa aatagccgcc gtttcttttt gtgcaccggc agcaaagcgt
    20281 ttgctatagc ggcgaaactc gttagccgcc tcttccaacg ccccggtcag tcgttcgcgt
    20341 tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca
    20401 tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcacg gattcgcgtc
    20461 tggcgatatt gcccaaacaa ggcagtcaac tgcgactgag aaagaatagc tgccatctgg
    20521 gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc
    20581 tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg
    20641 cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct
    20701 gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg
    20761 agcgttacag tgcgaccgcg tggttttttc agtccccggg tcgccatcag gtagtaacaa
    20821 cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg
    20881 tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact
    20941 atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca
    21001 gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct
    21061 ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac tggataccag
    21121 taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct
    21181 gcatcgccgc tcaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc
    21241 ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa
    21301 gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc
    21361 gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga
    21421 ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt
    21481 gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat
    21541 gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta cacactaata
    21601 acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg
    21661 tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa
    21721 cccggagcat tctgaataag acagatacag agattttaaa atattcatta tcagtcaatg
    21781 cattacatgt gatttgagta ataaaaaaga gtaattcgtg acccaggtca cacctctcat
    21841 ttacgggttg gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg ccactaagtt
    21901 aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atggaaacag
    21961 tagtgaaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt
    22021 ttattttcag tgaccagatt tggaaaaccc gttgcagtgt tgcgcaactc gattaccggc
    22081 aacctaaaaa gcaatataaa tcagagaatt gaacaacgca tgtggaaaaa gttacactgc
    22141 gaatattcgg cacttaattg ctgtttgttt tttaaccaag gtatcatgac atgtcccaac
    22201 ctcgcccact gctctctcct cccgaaactg aagaacaatt gttagcgcaa gcacaacaac
    22261 tttctggtta tacattggga gaactggcgg cacttgccgg gctggttacg ccggagaatt
    22321 taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag
    22381 ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg
    22441 atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta acgggcaata
    22501 gcggggtgac ctgggaaacc agccacgtgc gccacaagct caaacgcgtg ctgtggatac
    22561 cggttgaagg cgagcgcagc atcccgctgg caaagcgtcg cgtaggatca ccgttgctgt
    22621 ggagcccgaa tgaagaggaa gaccgacagt tacgcgaaga ctgggaagaa ttaatggata
    22681 tgattgttct cggtcagatt gagcggatca ccgctcgtca cggggaatat ttacagatac
    22741 gaccgaaagc agcgaatgcg aaagcgctga ccgaagctat tggtgcccgg ggcgaacgga
    22801 ttctgacgct gccgcgcgga ttttatttga agaagaattt caccagtgcg ctactggccc
    22861 gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca tataattacc
    22921 gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc
    22981 ctggcttgcg ctcggtacac tgacgctgct ggagatcgtt cttgggatcg acaatattat
    23041 tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct
    23101 ggggttggcg ggagccatgg tcatgcgtct ggcgctgctg gcatccatcg cctgggttac
    23161 gcgcctgacg aatccgcttt ttacaatatt cagtcaggaa atttccgccc gtgatttgat
    23221 cctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat
    23281 cgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca
    23341 gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc
    23401 agatcacctg tttattatga tggctgccgt ggtgattgcc gtaggcgtga tgatgttcgc
    23461 cgcgcgctcg attggtgatt ttgtcgagcg ccatccttcg gtaaaaatgc tggcgctctc
    23521 tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa
    23581 aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca acctgattcg
    23641 caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc gtactcactc
    23701 ctgttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc ggtttataaa
    23761 cagactatta tcataggtgg gccaaatgag gataaccgga tgaaaaaatg ggcagtaata
    23821 atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg
    23881 accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg
    23941 ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg
    24001 caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt
    24061 tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa
    24121 cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg
    24181 gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg
    24241 ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc
    24301 ccgaaactca agggctaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc
    24361 gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaaaggca
    24421 tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat gacagcctca
    24481 agcgtctgca gactgattac ctcgatcttt atcaggtgca ctggccgcag cgcccaacca
    24541 actgcttcgg caaactcggt tatagctgga cagattctgc gcctgcggtt tcgctgctgg
    24601 atacactgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt
    24661 cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc
    24721 cgcgtattgt caccattcag aacccctaca gtctgttaaa ccgcagtttt gaagtaggtc
    24781 tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg
    24841 gcacgctgac cgggaaatat ctcaacggtg caaaacccgc tggcgcacgt aatacgctct
    24901 ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg
    24961 atatagccag acgtcatggc ctggatcctg cacagatggc gctcgctttt gtacgccgtc
    25021 aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggagcagctg aaaactaacg
    25081 tcgaaagttt gcatctggag ttaagcgaag acgtattagc agaaattgaa gcggtgcatc
    25141 aggtttacac ttatccggca ccataagatt aacgccggtt aaaaccggcg ttaaatatta
    25201 ataacggcgc tgccagatcc acagcgccgt tattgccagc gcaaacagcg caccgaagcc
    25261 aatgccaatg ggcacgaccg ggatgcctac cattaccgcc agcgagtaaa tgcccagcat
    25321 caacaacatg gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc ccgccccgac
    25381 gcttttttta ccccgttcct gtagcaacgc attgagcgga acgacaaaaa agccccccag
    25441 cacgccaatc agcatcaaca aggcataggc tgacagcagc tcgtgttgca gggagaaaat
    25501 cagtaccacc acgccaatca aaatcccggc tggcatacag cgtgacacgg tttccagcgt
    25561 aactaacttc gctgccgcac cagcgccaac cacgatacca atcgctacca tcgcgttgag
    25621 ataggtcggc gtagcgttat cggtaatgcc cagcgccacc ggtacccaca gcaccaacag
    25681 gaaacgcagc gtgacacccg ctccccagaa taaactggtg cccaccagcg aaaaacgcgt
    25741 ttcaccattg cgccatagcg aggtgcaggc attcaggaaa ctgcgggtca tgctgatgag
    25801 attccaggac tgccccggac gcgccgccgc cagtttagga atgtagatat tggcaacgac
    25861 cgcaccaccg taggccagtg cgcatgcggc cagggcgacc aggacatgcc agtcagccag
    25921 tacaccaccg gctacggaac cgagcaaaat cgccgctatg gtagaagctt ccattaaacc
    25981 attagctttc actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg
    26041 tgaataggct gcagcaccaa cacccaccag cgtatagccg agaaacggat tgataccaaa
    26101 gcagatactg gctgcgccca gcagcttcag gccgttggca aacatcatca cccggccttt
    26161 ggcgaagcta tccgccacct gtccgacaaa cggcgcaaaa agaatgtaag cacctacaaa
    26221 caccatttgc aggatgggct ggctccactc cggataaaac tgcgctttca gtaacgccag
    26281 agtggcaaac agcagcgcat tatcgccaaa cgcagagaga aactgcgcca cgataaccgc
    26341 tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt
    26401 ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg
    26461 gcatctgttt cagatagcga atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc
    26521 gggcatactg ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg
    26581 cctcgccttt gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac
    26641 caagtgccag ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc
    26701 gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc gaaacgcaca atatcgccag
    26761 tgtcatacca gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta
    26821 cacctggctt ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc
    26881 gtccgccctc ttcgatacca ggaaccgaca acaggcgcgc atccattcct ggtagaatac
    26941 gccctaccgt accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcact
    27001 cggtcacacc gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttgg
    27061 tactttcttg tagtttttct gcgcctgcca ccacatagcg tagacggtag aagtcatacg
    27121 ggttggcgaa acgcgcgtag tgaccgagaa aagtcgaggt accgaacaac acggtgcaac
    27181 tgcggtcata caccaactcc ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca
    27241 cctctgcacc tgtgagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca
    27301 gcggtaacgc cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct
    27361 cgacattcgc cagaatgctt ttatggctat ggacgacgcc tttcggatgg ccttcagaac
    27421 cggaggtaaa aaggatcagc gcctcttctt ccggctgctg ttttacctgt gccagacgcg
    27481 gcatcagcaa atgagcgaag atccatactt tgtcggcagt ggtgacatct gcttttaaat
    27541 cttccagata gacccagcgc acctgagtaa gttgctccgg cagatgccag agtttgcctt
    27601 tatcgagaaa ctgacgggaa gtgaaaatgg ttttgatttc agccgccgta atagcactgg
    27661 tcaacccttt taccccggcg gtgtagttca tcattgcggg aatgcggcga cgggcgatgg
    27721 ccccaaaaat cactgccgca ctgatgcctg cattgggcag cattaagccg atgcgttcgc
    27781 cttcaacact gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc
    27841 gataggagtc cggggtaaag ttaacgtctt cgacacattt cttcccggct ccgaagcggt
    27901 acattgcgct cagtaaagat tcgtacagcg tttcacgcgg gcgcaccgcc atacgcgctt
    27961 ccatcattat ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg
    28021 catccggcat ctccacctgc gttggtggca aaatatgcag agtaatttgc gggaacaagc
    28081 gacgtttaac cagacctttc aggcggctga agtgcgtaag ttccgcccct tcaatacgca
    28141 caggaataac cgttgcacca gacttcgccg cgacaaatcc cgcgccatcg tagattttca
    28201 tcagcgagcc agtggtggtg atgcgtcctt cagggaaaat caccactggt cggccctgtt
    28261 ccaccagacg taccagatgt ttaatcgcca taggttgcgt cgggtcgaga ggaacaaagt
    28321 cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa
    28381 ataccggacg tacgggtaaa aacaatgcca gcagtatgcc atcaataaaa gagacgtgat
    28441 taggcgtgat tagaacgcgc tcgcccttca gtgcctgggt gtcacccgta acgcgaacgc
    28501 gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc
    28561 tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa
    28621 attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa
    28681 gagcgtaccg aataatctca ccaatcaata cctctgggat cttgattgtg gtctgcacga
    28741 cgactcttcg ccagtgagaa aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa
    28801 ttgtcggtta ctgttacaga ttgatgaccg g
//
LOCUS       DAZKJR010000056        28784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKJR010000056 DAZKJR010000000
VERSION     DAZKJR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.056"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..9715)
                     /gene="cdiA"
                     /locus_tag="ACHOWS_002463"
     CDS             complement(<1..9715)
                     /gene="cdiA"
                     /locus_tag="ACHOWS_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFQ0048020.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GKPVMNADGSLQALEVTEGSITINGAGLDGTWSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGNVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARE
                     GDIILSSSGKLVLKNSLAGGSTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQSRLVT
                     DKNLVLSSSGQIVQNGGELTAGQNAMLSALHLNQTSGAVNAAENVTLTTTDDTTLKGR
                     SVAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGAVQGNGLKVTATDLTSTGSIK
                     SGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLISDDVLTLSATQINNSGTLS
                     GAKELVASADTLTTTEKSVTNSDGNLMLNSASSTLAGETSAGGTVSVKGNSLKTTTTA
                     QTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVL
                     VASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLI
                     TGDNGLTLNTASLSNPGKITADTLNVRATTLDGDGLLQGAAALALAGDTLSQGRHGRW
                     LTAGDLSLRGKTLNTAGTTQGQNLTVQADRWANRGSVLATGNLTASATGQLTSTGDIM
                     SQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTLHHRSTDNSGTVTGL
                     SGLTLHSADGLTNSGALLSQNSLVLSAGDVTNSGRIQGQNITLDASSLTSSGAVQSAL
                     DLALTLSGDVIAATGSKITALGDARLSGKVLGNQGLISAKTLEVNGDSLSNSGEISGV
                     NSLNVTLSGNLQQHGKMLTGGALNVNARDISNSGQLQGADNRITASSLANSGRVQGES
                     GLTLTLLNALTNQTSGVLLSQNVSALSAPALTNDGTIQGNGKTTLSAATQAHNSGKIL
                     SGGELTFTTPDYSGSGWLQATDLLLNVAKLAGNGTVMAANQATLTGNSLTNRGLFQAA
                     QLNVNTQTITNSGTLLGNQGLTIKGNNLNNAGGKVFSGGDMLAEMVSLSGAGQLVALG
                     NLTLKLTRGLTAQGVIAANKQLSVSSQGDITNGATLQGNGITLNAAGRLTNNGQLTAG
                     NGTTALSGSGIAMNASGSLQAGGDVSLTSRGDITLDAFTGTTGSLMLTAAGAVINTAL
                     LYAGNNLSLFASTIRNHHGDMLAGDSLVMQKDVSGAANAEVINTSGNIETTRGDITIR
                     TGHLLNQREGINETKSYIPVENVAVPDGANSVSVRVGDLGEDGWGYYVKSWSGTAGGG
                     FDAWAVPTEKGATRKFLTGTTRVDVGATGGDARISAGNNLLIDADKLDNTGSHLLASG
                     FVSLSGSQLNNQSFFGYTQDEYNVYRYYGKLAMIPNDGHLQYGDASADDRVTFTLSGA
                     PEYVTRDTGQALRAVIQAGKNVTAVFSSDISNTSTTSNAGRITNTLAAPEINTPAEKN
                     ISPGMAQLAPDGTETLTVTAPDWTDTITRLTIGSGTDLASGIVEGNYPLPSGNNGYFV
                     PSTDPDSPYLITVNPKLDGLGKVDSSLFTGLYDLLRMHPGQAPRETDPAYTDEKQFPG
                     SSYFLDRLGLKPEKDYRFLGDAAFDTRYVSNYMLNQIGGRYINGVGSDTDQMRYLMDN
                     AARAQKALGLKFGVALTADQVAALDQSILWYKAVTIKGQTVMVPEVYLSPKDVTLQNG
                     SIISGQNVHLAGGNVTNSGSTLMAQNNLTIDSADSLGNLESGLINAGGALGLKAMGDI
                     NNISATITGKTVRLESLAGNVNNLTRYSHWQLDAPEDSLALKHTYTGSIASVSAMDSL
                     DIRADKNISVTGAEISAGDRAALIAGNDLSLNAIDRVSSRRHANSESHQRSAGLTTIT
                     AGDSVMLSAGRDVSSQGAGIAAEDNITVRAGRDVNLLAEESVTGSSSYSKKKTVIDET
                     VRQQGTEIASGGDTTITAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLET
                     KKKSGGFLSKKTTHTISEDSATREAGSLLSGNRVTVNAGDNLTVEGSDVVADRDVSLA
                     AGNHVDVLAVTSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSAS
                     TIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSG
                     LTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGD
                     PNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGS
                     QLKAGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVN
                     AAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQ
                     DTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDI
                     TVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDK
                     FQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNPADLSRDPAHANDSI
                     SPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQ
                     RQEYLAKLRDTPEYKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPEL
                     AHLLKSTEKDPVVNAIAHAILGGAVAAMQGNNVAAGAAGAATGEMAARAIAGMLYPGV
                     KQSDLSEEQKQTISTLATVSAGLAGGLTGNSSASAAVGAQSGKNAVDNNYLSVSEKTE
                     LEIAKQTLKNSKNPAEREKAQQKYDALLEKDIASDKEVIAACGNGNAGSSACASARLK
                     VIASKEGYEDGPYNSKYSQQYADAYGQIVNLLDITSVDVQNQQQVKDAMVSYFMATLG
                     VDQKTAQGYVETTQGLEIAAASMTPLFGQAVANKITALVDKANKYPSGIGFKINQPEH
                     LAQLDGYSQKKGISGAHNADVFNKAVVDNGVKIISETPTGVRGITQVQYEIPTKDAAG
                     NTTGNYKGNGAKPFEKTIYDPKIFTDEKMLQLGQEAAAIGYSNAIKNGLQAYDAKAGG
                     VTFR"
     gene            complement(9728..11506)
                     /locus_tag="ACHOWS_002464"
                     /pseudo
     CDS             complement(9728..11506)
                     /locus_tag="ACHOWS_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            12006..12395
                     /locus_tag="ACHOWS_002465"
     CDS             12006..12395
                     /locus_tag="ACHOWS_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048021.1"
                     /translation="MKRYIKWLAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDNSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            12459..13517
                     /locus_tag="ACHOWS_002466"
     CDS             12459..13517
                     /locus_tag="ACHOWS_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFQ0048022.1"
                     /translation="MYEQDTLSTLDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEQTVLNEYAVSVLLDMGLSGRIITCKEGNYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARASWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFQAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            13558..14280
                     /locus_tag="ACHOWS_002467"
     CDS             13558..14280
                     /locus_tag="ACHOWS_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFQ0048023.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRYQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALMIESGDDWQCETQSAIAVNETTLPQSMLFLQH
                     YLQNAGAFSLSGERVQWRWSRR"
     gene            14277..15098
                     /locus_tag="ACHOWS_002468"
     CDS             14277..15098
                     /locus_tag="ACHOWS_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0048024.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSHEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            15073..15330
                     /locus_tag="ACHOWS_002469"
     CDS             15073..15330
                     /locus_tag="ACHOWS_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFQ0048025.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTNAPLFGDGLGLDSIDA
                     LELGLAVKNEYGVVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            15342..15593
                     /locus_tag="ACHOWS_002470"
     CDS             15342..15593
                     /locus_tag="ACHOWS_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0048026.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVKRLLQEG"
     gene            15598..16179
                     /locus_tag="ACHOWS_002471"
     CDS             15598..16179
                     /locus_tag="ACHOWS_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048027.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLAHNSLHWLSPLMALL
                     LLLRLRQPRRQAGPLQAVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            16176..17534
                     /locus_tag="ACHOWS_002472"
     CDS             16176..17534
                     /locus_tag="ACHOWS_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFQ0048028.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCILSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            17521..17874
                     /locus_tag="ACHOWS_002473"
     CDS             17521..17874
                     /locus_tag="ACHOWS_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFQ0048029.1"
                     /translation="MIRHEIERHQVQLQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            17865..19541
                     /locus_tag="ACHOWS_002474"
     CDS             17865..19541
                     /locus_tag="ACHOWS_002474"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0048030.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTCYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQGLQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            19545..19967
                     /locus_tag="ACHOWS_002475"
     CDS             19545..19967
                     /locus_tag="ACHOWS_002475"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0048031.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            19964..20569
                     /locus_tag="ACHOWS_002476"
     CDS             19964..20569
                     /locus_tag="ACHOWS_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFQ0048032.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLSDDERQRFAAQ"
     gene            20538..22856
                     /locus_tag="ACHOWS_002477"
     CDS             20538..22856
                     /locus_tag="ACHOWS_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFQ0048033.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVVLLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILMIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLSVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLGRLETFTTSLEKA
                     KKDGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALQQEIASYYPGGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            22853..23437
                     /locus_tag="ACHOWS_002478"
     CDS             22853..23437
                     /locus_tag="ACHOWS_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFQ0048034.1"
                     /translation="MIKSTFWRALALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLSGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAKG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPTGWTLRDNGNKRELRNASGKL
                     VTEIIYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            23439..24608
                     /locus_tag="ACHOWS_002479"
     CDS             23439..24608
                     /locus_tag="ACHOWS_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFQ0048035.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTNGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDIG
                     YINLHGTATQLNDQIESMVVNALFGKRIPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            24605..25069
                     /locus_tag="ACHOWS_002480"
     CDS             24605..25069
                     /locus_tag="ACHOWS_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFQ0048036.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            25069..25800
                     /locus_tag="ACHOWS_002481"
     CDS             25069..25800
                     /locus_tag="ACHOWS_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFQ0048037.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            25797..27026
                     /locus_tag="ACHOWS_002482"
     CDS             25797..27026
                     /locus_tag="ACHOWS_002482"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFQ0048038.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARSATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(27200..27469)
                     /locus_tag="ACHOWS_002483"
                     /pseudo
     CDS             complement(27200..27469)
                     /locus_tag="ACHOWS_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27866..>28784)
                     /locus_tag="ACHOWS_002484"
     CDS             complement(27866..>28784)
                     /locus_tag="ACHOWS_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0048039.1"
                     /translation="VSDKLREKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTD
                     KASSRFMQSLQQVLNKNEFQLLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADK
                     THQILEKTNIPTINVIGSPFSPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMD
                     NRLQRQQLNGWHKAMLSHYQNTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSH
                     EEIALGIMFECQRRLLKIPGNIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLL
                     IGLLNNNHDESEESRIVQFNYQIELRQST"
BASE COUNT         6296 a         7965 c         7512 g         7011 t
ORIGIN      
        1 ctctgaacgt aacaccaccg gcttttgcat catatgcctg taatccattt tttattgcat
       61 tggaataacc aattgccgct gcctcttgac caagttgcaa cattttctca tctgtaaata
      121 ttttaggatc ataaatagtt ttctcaaaag gcttggcgcc atttccttta taattaccag
      181 tcgtattccc agcagcatct tttgtgggga tttcatactg aacctgagta atacctctga
      241 ctcctgttgg tgtttcagaa ataattttca caccattatc aaccacagct ttattgaata
      301 catcagcatt atgtgcaccg ctaattcctt tcttctgcga atatccgtca agttgagcta
      361 agtgttccgg ctgattaatt ttaaaaccaa tccctgaagg atatttattc gctttatcaa
      421 ccaatgcagt aattttatta gccacagcct gaccaaataa tggtgtcatt gaagccgctg
      481 caatttctaa tccctgcgtc gtctcgacat aaccttgtgc tgttttctgg tcaaccccca
      541 gcgttgccat gaagtaactc accatcgcat ctttcacctg ctgttgattt tgtacatcaa
      601 cactggtgat atcgaggaga ttgactatct gaccatacgc atcagcgtat tgctggctgt
      661 atttcgagtt ataagggcca tcttcgtacc cttctttcga cgcaataact ttcagtcttg
      721 cgctggcaca cgctgaacta cccgcattac cattaccgca ggcagcaata acctccttat
      781 cactggcaat atctttttca agcaatgcat catatttctg ctgtgccttt tcacgctctg
      841 ccggattttt actgttttta agcgtctgtt ttgctatctc aagctctgtc ttttcagaca
      901 cgctcaggta gttattatca acagcattct tcccgctctg tgcgcccact gctgctgatg
      961 cgctgctgtt tccggtaaga ccgccagcca gccctgctga cacggttgcc agcgtactta
     1021 tcgtctgctt ctgctcttca ctgaggtctg actgtttcac gcccggatac agcatgccgg
     1081 caatcgctct ggctgccatc tctcccgttg ctgcgcctgc tgcaccggca gcaacattat
     1141 taccctgcat tgccgctact gcgccaccca gaatggcgtg agcaatggca tttactaccg
     1201 gatctttttc tgttgatttc agcaggtgcg ccagttccgg ggccgaagct ccggccagag
     1261 cacccgctaa attcccccca gccagtccct gaagcgcggc tgtcgcggcc tgtatacccc
     1321 gctgaatctc actgccagtg ccatactttt cctgttcctt tttgtattcc ggcgtatcac
     1381 gcagtttcgc aagatattcc tgacgttgtt tctctgttgc attagcagga agtgtttcgc
     1441 cggttgcttc cttcgcggcc ttcaacgcat tcagttcccc ctgcgtccgc gcgatatccg
     1501 ccacctgact gcctatgtca ctgataagcc ccactgtctg cagacgcctc tgctccttct
     1561 ccttgtcaaa tatcgggctg atactgtcat tagcgtgcgc aggatcacgg ctgaggtctg
     1621 ccggattctg cttctgattg tccctgtccc ggatggtgat agtgccttct gccactgcgg
     1681 cctgagtcgt tccttccgca tgtccgctgt gacctccggc ggatatcatg ccacccggca
     1741 tgttaccctg aaatttatcc ccgaagctgc caccaccgct cagactgatc ccactgtgac
     1801 tgactttata atccgcttcg ttgtgaaggt cactgaaccc cagcgttccg gtatccaggt
     1861 ggtttttatc cggtgtggca gtggaggcaa tcaccgcacc atccagttgg gtatgtctgc
     1921 ccactgtgat gtcgaagcca ccatcaccgg caaacattcc ggtttgttca gcaacggagt
     1981 caaagcggct cttcatctta tcccgggagg cagcgatgta acctgagccg gtcatggagc
     2041 caaaggtaaa actgccgccg gcagccacgc tggtctgttt actgtcgtac ttactggtgt
     2101 cctgctggct gctaatcagc aggtcgtggc ccacatcggc gataatcctg ttgccgttga
     2161 cctgagcacc gttcagtacc gtatcccgac cactgttgat ggtgacggtt ttaccgctgt
     2221 ctgttgtggt ttcagtccac tcagtaccgt tacctttctc gctgcctttt gccgcattaa
     2281 cgctggcaaa gacgctgata ccggcaccgt taccaccggc accgatactg acacccacgc
     2341 caccgccact gctgctgttc ctgcccgttg ttttttgtgt gtttgcagcg ccactcaaca
     2401 gaatatcatt cgcggcatcc aggcttgtgt taccaccggc cttaagctgg cttcctgcaa
     2461 tcacaatatc tccgcggtta tcgcccctgt ttttgccggt tgcgacaaca gacagattat
     2521 tcccggcatt cagcgtactg ccggatactg tgtcactttc agaatgttgt tgtgatttcg
     2581 atttctgagt ggtgagcgac aggctgactc ccgtcgcatt cgggtcaccg gttgcggtgg
     2641 ccattgccgc agcctgtccg gcctgcacac cagacagcgc tgtctttgtg gcctgcaggg
     2701 ctttcagacg actgtcactg ctctccttcg tttcctgtgc actggtgacc gcattattga
     2761 tggcactgcc cactgtgccg gaaagggcaa ccgtcagccc gcttttcttc tgctcaaatt
     2821 tttcgtccac agtacgacgg tcatgccccg ggtcaaccac cacactgtca ccggtaatgc
     2881 tgatatcccg gttcgcaatc acatccgaac cgctgatatg agcctgtttg cccgcggtaa
     2941 tactgacatt accggcagtg gagccgatgg tactggcact ctgactctgc gttgtcccgg
     3001 cctcgcggcg gtcgtgcgtt gtcttactgc tgccgatggt gaagccgata ccgccagtac
     3061 ccatcagacc cgatttcttc gtttccttaa agcgccagga cgtatctgta ctggtgacag
     3121 caagaacatc aacatggtta cccgccgcca gtgacacatc ccggtcagcc accacatccg
     3181 aaccctctac cgtcaggtta tcaccggcgt taacggtcac gcggttcccc gacagcaggg
     3241 agcctgcttc acgggtggca ctgtcctcac tgatggtgtg ggtggttttc ttactgagaa
     3301 aacctccgct ttttttcttc gtttccagat agtgatagtc actttctgtc gccgtggtca
     3361 gggcaacatc acgaccggca ttcacgctga tattgccggt tgcggtaacg gatgacgcaa
     3421 cagcggtgat atcccgtcct gcagtaatgg tggtgtcacc accgctggcg atttccgttc
     3481 cctgctggcg aacggtttca tcaatgaccg ttttcttttt cgagtatgaa ctgctgccgg
     3541 tcacggactc ttccgccagc agattaacgt cgcgcccggc ccggacagtg atgttgtctt
     3601 ccgctgcgat acctgcaccc tgactgctca catcccggcc tgcactcagc atcacactgt
     3661 cgccggcagt aatggtcgta agcccggcgc tgcgctggtg agactcactg tttgcatgac
     3721 gtctgctgct taccctgtca atggcattca gactgagatc attgccggca ataagtgctg
     3781 cgcggtctcc ggcactgatt tctgcccctg tgaccgaaat gtttttatct gcccggatgt
     3841 ccagactatc catggcactg acagaggcaa tgcttcccgt ataagtgtgt ttcagggcga
     3901 ggctgtcttc cggtgcatca agctgccagt gtgagtaccg ggtgagatta ttgacattgc
     3961 cggcgagact ctccagccgg acagtcttac cggtaatcgt ggcactgata ttgttgatat
     4021 cgcccatcgc tttcagtccg agagcgccac cggcatttat aagcccggac tcaaggttac
     4081 ccagactgtc agcgctgtcg atggtgagat tattctgcgc catcagggta ctgccactgt
     4141 tagtgacatt ccctcctgcc aggtgcacat tctgaccgct gataatactg ccgttctgca
     4201 gcgtcacatc tttcggtgac agatacacct ccggcaccat caccgtctga ccctttatcg
     4261 tgacggcttt ataccacaga atactttggt cgagagccgc cacctggtct gctgtcagtg
     4321 ccacaccaaa cttcagaccg agcgccttct gtgcacgggc agcgttatcc atcaggtagc
     4381 gcatctggtc ggtgtctgaa ccaaccccgt tgatatagcg ccccccgatc tggttcagca
     4441 tatagttcga tacatagcgg gtatcaaaag cggcatcccc caggaagcga taatcttttt
     4501 ccggtttcag cccgagccgg tcaagaaaat acgatgagcc cgggaattgt ttttcatcag
     4561 tatacgccgg gtctgtttca cgtggtgcct gcccggggtg catcctgagc aggtcataca
     4621 gtccggtaaa cagactgctg tccacctttc cgagaccatc cagtttcgga ttcaccgtaa
     4681 tcagatatgg actgtcaggg tccgtggacg ggacaaaata accattgtta ccggaaggca
     4741 ggggatagtt cccctccacg atgccggaag ccaggtccgt gccactgccg atcgtcagac
     4801 gggtaatggt gtcagtccag tccggggctg tgacggtcag cgtctctgtg ccatccggcg
     4861 ccagttgagc cattcccggg ctgatatttt tctctgccgg cgtgttaatt tcaggtgccg
     4921 caagggtatt tgtgatccgt cctgcatttg aggtggtgga tgtattactg atatcactgg
     4981 aaaaaacggc agtgacattt ttgcctgcct gaatcacggc tctcagagcc tgaccggtgt
     5041 ctctggtgac atattccgga gctccggaga gagtaaaggt aacgcgatca tcggcagaag
     5101 catctccata ctgcagatgc ccgtcgttgg ggatcatggc cagtttgccg taatagcgat
     5161 acacattgta ttcatcctgt gtatagccaa agaaggactg attattcagt tgtgaacccg
     5221 acaggctgac aaacccgctg gcaagaagat gacttcccgt attatccagt ttgtccgcgt
     5281 caatcagcag attattcccg gccgaaattc tggcatcccc ccctgttgca ccaacatcga
     5341 cgcgtgttgt gccagtcaga aatttccggg tggctccctt ttcagtcgga acggcccatg
     5401 cgtcaaaacc accaccggca gtaccgctcc aggacttcac ataatatccc catccatctt
     5461 cacctaaatc gcccactctg actgaaacag aatttgcacc atcaggtact gcgacattct
     5521 caaccggaat gtatgatttt gtctcattta tgccctcccg ctgattcagt aaatgtccgg
     5581 ttctgatggt gatatcaccg cgggtggttt cgatattccc ggaggtatta atcacttcag
     5641 cattcgcggc accactgaca tctttctgca tcaccaggct gtcacccgcc agcatatcgc
     5701 cgtgatggtt ccggatggtg ctggcaaaca gcgagaggtt attgcccgca tacaacagtg
     5761 cggtgttaat gactgccccg gcggccgtta acatcagact acccgtcgtg ccggtaaaag
     5821 cgtcgagagt gatatctccc ctgctggtca ggctgacgtc cccgcctgcc tgcaggctgc
     5881 cgctggcatt catcgcaatc ccgctgccgc tcagtgccgt ggtgccgttg ccggcggtca
     5941 gctggccatt gttggtcagt ctgcctgcag cattcagcgt gataccgttg ccctgcaatg
     6001 tggcgccatt ggtaatatcc ccctggctgc tgacggagag ttgtttattt gcggcgataa
     6061 cgccctgtgc ggtcaaacca cgggtgagct tcagcgtcag gttaccgagt gcaaccagct
     6121 ggcccgcgcc gctgagcgaa accatttcag caagcatatc accgccgctg aacaccttcc
     6181 cgccggcgtt gttcaggttg ttccctttta ttgtcaggcc ctgattaccc agcagcgtgc
     6241 cgctgttggt gatggtctgc gtgttgacgt tcagttgtgc ggcctggaac agaccacggt
     6301 tggtcagact gttacctgtg agcgttgcct gattcgccgc catcacagtg ccattaccgg
     6361 caagcttcgc cacgtttagc agcaggtcag tggcctgtag ccagccactg ccactgtagt
     6421 ccggcgtggt gaacgtcagc tcaccgccgg aaagaatctt gccactgttg tgggcctgcg
     6481 ttgccgcgct gagcgtggtt ttaccgttgc cctgaatggt gccgtcattg gtcaacgcgg
     6541 gtgcggaaag cgctgatacg ttctggctga gaagcactcc gctggtctgg ttggtcaggg
     6601 cattcagcaa tgtaagagtc agcccgctct cgccctgaac acgacctgag ttggccagcg
     6661 aactggcggt gattcggtta tccgccccct gaagctgccc actattgctg atgtctctgg
     6721 cgttgacgtt taatgcaccg cccgtcagca ttttaccgtg ctgttgcaga ttaccactga
     6781 gtgtcacgtt caggctgtta accccactga tttcgccact gttgctgagc gaatcgccat
     6841 tcacttccag cgtcttcgcg ctgattaatc cctggttacc cagcactttg ccgctcagac
     6901 gggcatcgcc cagggcggta attttgctgc cggtggcggc aataacatcg ccactcaggg
     6961 tcagagccaa atccagcgca ctctgaacag caccgctgct ggtcaggctg ctggcatcca
     7021 gagtgatgtt ctgcccctga atccggccgc tgttggtcac atctcctgcg ctcagcacca
     7081 gcgagttctg gctcagcagc gcgccactgt tggtaagccc gtccgcgctg tgcagggtca
     7141 gaccgctcag ccccgtgacc gtaccgctgt tgtcagtgct tctgtggtga agcgtgacat
     7201 ggttaccctg gacagtgccg ctgttatcca gtgagtttcc atcaagggag agcgtgccgg
     7261 ccgaaagcag actgccccgg ttgtccgtgg tggctgcgtt cagcgtggtg tcaccctggc
     7321 tcatgatatc gccggtactg gtcaactgac cggttgccga agcagtaagg ttaccggttg
     7381 ccagcacgga accacggttc gcccatctgt ccgcctgcac ggtgagattc tgtccctgcg
     7441 tggtccctgc ggtattcagt gttttacccc ggagggagag gtcgcccgcc gtcagccagc
     7501 gtccgtgacg accctgtgag agggtgtcgc ctgcaagcgc cagtgcagcg gcgccctgca
     7561 acaggccatc gccatccagc gtggtcgccc tgacgttcag cgtgtcagcg gtgattttac
     7621 ccggattgct gagggaggca gtgtttaacg ttaaaccatt atcaccggtg ataagcccgc
     7681 tgttgcggat gtccggtata tccagcgtca ggtctgcagc actgtacagc gtgccgttct
     7741 gctgattatc aagcctctgt gtgttaacgg tgagtgaggc ctccccctgc aacagaccgc
     7801 tgttggtcag ggtctgtgac tgtgtattca gggcggaagc aacaagtacg ccgctgctgg
     7861 tcagttccgg cgcactgagg ctgagcgacg gggcgctgct tttcccgctg tgggtgagct
     7921 tttcactggc gttcagggtg aggatatctc tggcagcctg tgttccgtca agctgtgcgt
     7981 tctgcacatc cacgctgaca ctgttgccct gcgtctgcgc agtggtcgtg gtcttcagac
     8041 tgttgccttt tacagacacc gtgccacccg cactggtttc acccgccagt gtggaagacg
     8101 cgctgttcag catgaggtta ccgtcactgt ttgtgaccga tttttctgtg gtggtcagtg
     8161 tgtctgcaga agccacaagt tcctttgccc cggagagggt accgctgttg tttatctgcg
     8221 tggcactgag cgtcagcacg tcatcgctga tgagtctgcc gcggttttcg agtgtaccgc
     8281 tgacggtaac gcgggcactg tcttttgcac cggcatcacc ggacagtgtg gcattgcggg
     8341 cgctgatatc gagtgtgctg ccacttttaa tactgccggt gctggtcagg tcagtggctg
     8401 tgactttcag gccgttcccc tggacggcac cggtattact gaatgtcccg gttttcaccg
     8461 tggcatcgcg cccggcaacc agtgtcccgc cgttgttcag gctgccggag ctgacggtga
     8521 gtgttttccc ggcaacgcta cggcctttca gtgtggtatc atcggtggtg gtaagggtga
     8581 cattttcagc tgcattcacg gccccggaag tctggttcag gtgcagtgca ctgagcatgg
     8641 cgttctgtcc ggcagtcagt tcaccaccgt tctgtacaat ctgcccggat gaagacagca
     8701 ccagattttt atccgtcacc agacgggact gattcagtgt cagtcccgtt gtcccggtaa
     8761 cgctgagatt tcctccggct ttgttatccc ctgaaagtga gacatccgtt ccggttacgg
     8821 tggtactgcc gccggcaagg ctgttcttca ggaccagttt tccggaactg ctcagtatga
     8881 tatcgccctc gcgggcataa aggttaccaa gattaacccc gacaccactt tcagtggagg
     8941 tcagatgaat acgcctggcg tacattccac cgagagcgcc ggtatcaacg gcaactttcg
     9001 gcacattacc ttcgcccttc agggcactga cgcgaccatc tgcagttatc cggttagcgc
     9061 ctgcagtgac agttaaatcc ttcgcatgaa gcgcggcatt cacttccgtt gcacgggcaa
     9121 taatggatac ggcatcgctc caggtgccgt ccaggcccgc gccattgatg gtgatactgc
     9181 cttcagtcac ctccagcgcc tgcaggctgc cgtcggcatt catcacaggt ttgcctgtgg
     9241 tgagcgtcgc gtgcggcgtg ttgataaaac cacagccgtc acaggtgata ccatacgggt
     9301 tggcaaccat cacattcgcc gctttgccgg ccacttccgt atagccctgc agcaatgaac
     9361 ggttaccgcc agtcacttcg ttgatgatac ccttcgcttc cccgcccgct ttcaggttcg
     9421 ggttattctg tatcagtcca ccaagctgcg tcggattaag cttaccggtg gcattattga
     9481 gaatcagccc ttccttcccg acgttgtaat ccgtaaaccg gttatgcgaa atcccggccc
     9541 cgttcggcgt ggcaatgttc acgaccggca caccatttgc cgctttatcc attccggccc
     9601 cgttttgtgg ggtgatgacg gcccccacag ccggtaacaa cggctgcccg gcgataatcg
     9661 tactgataag gtaactcagc aggcggtaag tgaagcgaac gggaggctga tgcatggtaa
     9721 taatccctta aaacgcgacg gcaacgcgcc agtaaaccgt gagatggtca ggggcaagcc
     9781 agtccgggta aaccagaggc agtccggcag tgaacgaacc ggaaacatgg ccactggtgg
     9841 tgctgagccc ggcagcagca ccccacagcg tgccggacga gtacaggtca tctctgtcag
     9901 agtgcagcca gccgccgtcc agtgcagtca ctgcacggac tgtccccaca tatggcaggg
     9961 agaacagaga ccaggacagc tcatttcgca gataaccgcc gttattaccg gagatatact
    10021 gctccttaaa gccacgcact gaactctccc ccccgaggct cagttgttcc acaccatgaa
    10081 gacggtccgg tgaccactgg gcataagcgc tggtcagcca ccacaccctg tccgtgacgg
    10141 ggcgctgaaa actggcgctt aatgaccatt tccggaactg atttacgggc aggtctcccc
    10201 ttttcccgtg gtcgctttct gcgccgaacc agggcatccc ccgtgtgaat accggattca
    10261 gtgttccgac accccccaga aacttgtgtg tgtgattcag cccgacagaa aatgaagtga
    10321 gtttacggct gctgccctga agcagaacat catccagata attgtgaata atgcggtgct
    10381 gcagacctcc ggtcagtgct gtcttcatgt ccccgttacg gaacaggaca tgcgacagtc
    10441 ccagccgatg agtctgcagg tctcccgtgg aacgccaccg ccagccccgg ttatcaatgg
    10501 tgctgaggta gtcactccat gaatacgtgt aatccaccag ggtatagcca tacggcagac
    10561 tgacaccggc ggcaaaattc ctcgcatcat gtgacaccga aaagtcactg ctccgtcccc
    10621 cgctgacaaa ccagttgtca gccagcccca gcggattatt aaaggaaagg acaccattta
    10681 actgccccgt accggtattc ttctgtccgc tgttgtcgat gcccacgctc cctgtgacag
    10741 gccattccgg cgatgccgtc agtgtcacca ccgaccatcc ctcacggtca ccgggcgata
    10801 tttaaatctg taccggctcc gtacgcagac ggttaatctg ctccatcccc tgctcaatat
    10861 cccgcaggtt cagaaccttc ccctccattc ccgggaaaac catcttcagg gtgcggccag
    10921 gaaggtcagc gccttccgcc cggatttgct gcagcctgcc ttccatgacc gttatgtgca
    10981 gtacgccccc tgaaaggtcc tgctctgtca gaaaggcccg gctggtgata tatccccggc
    11041 gtatatagcc gtccgtcacg gcatccgtga ccgcggtcag ccccgtgata ttcagacact
    11101 gattcaccca cggtgccacc agtctgtcgg tttctgcaga cgtcagtcgg gtggccccac
    11161 tgacaacaat gcgtgacacc gtaaagcagg gaccttcagt accggcagac atttccggag
    11221 acggtatgac agtcagcggc gcactgcgct ccagcgcatc acgctgacgc tggttttcat
    11281 ccagcaactg ctgctgttgc tgctgaattg cactgcggtc acccggagac agcatggctg
    11341 ccggagacga aaatgcgggg ataaaaaaac cagaaaaaac ggagagggag agataacata
    11401 tccccgaaga ggcgcgggcg cgccggaata tatcataaca ggggacagac acagcccgga
    11461 ataaaagaga cgttttcgtc agaatgttgt tctgccggtg ctgcatgttc tccccgtatt
    11521 ctggcctgtg taatgtcagt gtaaattcat gtcaaaaatg tgtcagcggg aaatttacac
    11581 ctgaaaaagt ctgtcatgaa acagctttat gacaagatat caaaaaaaat gtcaaaaacg
    11641 tgatctttct tcagcttttc agaatattga gagcctagag gtcaatataa attctgggac
    11701 acatatgcat ttaccattaa aaccggcaca acaggctgtt cagtctcaat ccatgctttc
    11761 tgttttatcg atctcagaat atcggcagac aaataaaaaa ggaatgtcga gagagctaac
    11821 tctagatcac tctccacctc acaagccatt tacaaaaatg atacaggcat atctttggtc
    11881 cctgccacaa ccagacattg acaatccaat ataaccccaa atatatttcg gatacgaagc
    11941 ttgggattta tacagacatt tactatttaa ttataaaatt tgtattacat acataagagg
    12001 cactaatgaa acgttatata aaatggcttg ccatcacaat ttttatcagt atgttgagtg
    12061 cctgtgcccg tacggctcca gtgcaacaga taagcaccac tgtcagtgtg ggtcatactc
    12121 aggagcaggt taaaaatgcc attttgaaag caggtgcaca gcgcaagtgg attatgacgc
    12181 aagtgtcccc tggagttatt aaagctcgct atcaaacacg aaatcacgtt gctgaggttc
    12241 gcattacata tacagctact tactataaca tcaaatatga caatagcctg aatctgcagg
    12301 cttctgatgg aaaaattcat aaaaactata accgttgggt gcgtaacctg gataaagata
    12361 tacaggttaa cttgtctaca ggagcaacgt tatagtgatg ctgatatcgg gctggaatag
    12421 cccgatatca ttaaacttct ctataagaat ggttatatat gtacgaacag gacacactca
    12481 gcacactgga tgccatcacg gaggcacaac ggattgcttt tgctcctatg cttttccagg
    12541 cagctttgtg cctgcgcaat gcaggaatat tagattattt ggatcaacag ggtaaacagg
    12601 gagctcccct taacgcgatt actgaacaga cagtcctgaa tgaatatgcc gtgagtgtgt
    12661 tactggatat ggggttaagc gggcgaatta ttacctgtaa agagggaaat tattacctgg
    12721 cgaaaatcgg tcattacctc ctgcatgaca ctatgactcg cgtgaatatg gatttcaccc
    12781 aggatgtctg ttaccagggg cttttcttcc ttgccgattc actcaatgaa ggaaaacctt
    12841 ccgggttaaa agtatttggc gactggccga cgatatatcc ggcactttcg caattaccag
    12901 atgctgctcg tgctagctgg tttgcatttg atcattacta ttcagatggc gcatttaacg
    12961 ccgcactgcc ttatgtattc gccaacaacc ccacaacctt gtacgatgtg ggcggcaata
    13021 cgggtaagtg ggctttgcgc tgctgcaaat acaatgaaaa catcgccgtt acgttattag
    13081 atctgccaca acaaatcgta ctggcgaaag aaaatatcgc gaatgcaggt ttttctgatc
    13141 gcatagattt ccaagcggtg gatatgctaa gcgatgcccc gttaccaggc gaggctgata
    13201 tctggtggat gagccaattt ttagactgtt tttcacccga acaaataatt agcatactca
    13261 gcaaaatcgc cagcgtaatg aagcctggcg caaaagtttg catcatggag ttgttctggg
    13321 atgcgcagcg gtttgaagca gcatcgttta gcctgaacgc gtcctcactt tattttacct
    13381 gcatggcaaa cggtaacagc cgtttctata gtgcagagaa attttacgac tatctgaata
    13441 aagcaggatt ccaggtagca gagcgccacg ataatttagg cgttggacat acacttttga
    13501 tatgccaaaa gaaataatcg tcacttaata attttggttt accgcgggca cgcgttgatg
    13561 aaatttgcat taaatattac caactggcag gcgctggcgc ctggacttag cgatgtgcag
    13621 caatggcaag catggtcgcg ccagccgtgg gcgattgacc ctgccgcgcc gctggcaaaa
    13681 ttaagtgaac tgccaatgat gactgcccgt cgtctcagtt caggcagcaa actggctgtt
    13741 gaatgcggtc tggcaatgct acgccgctat cagcctgacg ccgttttgta taccagtcgt
    13801 catggcgaac tggagcgtaa ttaccgcatt gttcatgcac tggcaaccga acaggcgctt
    13861 tctccgacgg acttcgcgct ttccgtacac aattcttccg tgggtaacct gaccattgcg
    13921 gccaaacagc cgatcgtttc gtcatcgctt tcagctggtc gcgacagttt ccagcaaggt
    13981 ttgtgtgaag tgcttagcct gctacaagcg ggataccagc gcgtgttgat ggtcgatttt
    14041 gacggttttc tgcccgagtt ttatcaccca caactgcctg cagaaatgcc gacctggccg
    14101 tacgccgtcg cgctgatgat tgaatctggc gatgactggc aatgtgaaac gcagtcagcc
    14161 attgcggtaa atgaaacaac actgccgcaa agcatgttgt ttttacagca ctatttacaa
    14221 aatgcaggcg cattttcact gtctggcgag cgcgtgcaat ggcgctggag ccgcagatga
    14281 ataagctgat gtcgcgcctg ggatgggcat ggcgactggt catgaccggg ttatgttttg
    14341 ccctgtttgg tctcggcggc ctgttgcttt cagtcgtatg gtttaacata ttgttagttt
    14401 tggtatggga tacttcccgc cgccgtcgtt tggcgcgtcg cagcattgcc gccagttttc
    14461 gcctgttttt aactgtcgcg aaagggcttg gcgtgctgga ttatcgtatt gatggcgctg
    14521 aaattttacg tcaggaacgc ggttgcctcg tcgtagccaa tcatcccacg ctgatcgatt
    14581 acgtgctgct ggcgtcagtc atgccagaaa ctgactgcct ggtgaaaagc gcgctgttaa
    14641 aaaatccttt tcttggcggc gtcgtgcggg cggcagatta tttaatcaac agcgaagctg
    14701 aaaccctgtt accccgctgc caacagcgac tggcacaggg tgacactatc ctgattttcc
    14761 ccgaaggcac acgaacgaaa cccggtgaaa aaatgacatt acagcgcggt gcagcgaata
    14821 ttgccgtgcg ctgcgccagc gatttacgca tcgtgacaat cggctgtagt gagcatctgc
    14881 tggataaaca aagtaaatgg tatgacgttc cgcctgccag gccgtttttt acggttgaag
    14941 tccatgggcg ggtagaaatc aataaatttt acgatgcaac ttcacatgag ccggcgctgg
    15001 ctgcaaggca gttaaaccga catcttttgc ttcaattaca acaaggttgt ttacctctct
    15061 caggaataaa taatgcaagc gctttatctg gaaattaaaa atctcatcat cagcaccctc
    15121 aatctggacg agttgactcc tgatgatatt gataccaatg cgccactgtt tggcgacggt
    15181 ttagggctgg actctatcga tgcactggaa ctggggctgg cagtaaaaaa tgagtacggc
    15241 gtcgtgctct ctgcggaaag cgaagaaatg cgtcagcact ttttctccgt cgccactctg
    15301 gcctctttca ttgctgcgca acgtgcctga ggaaagccat catgacagac caacaaacca
    15361 tttatcagga agtctcagcc ctgctggtta agctgtttga aatcgatccg caggacatca
    15421 aacctgaagc gcgcctgtac gaagatctgg aactggacag catcgacgcc gttgacatga
    15481 ttgtgcacct gcaaaagaaa accggtaaga aaatcaagcc ggaagagttt aaagcagtgc
    15541 gtacagtcca ggatgttgta gaggctgtaa aacgcctgct acaagaaggc taatttgatg
    15601 tcgggcattc gctcgctacc gatgattaag ttgctgaccg gattattgct gctcgcctgg
    15661 ccttttttaa tctggctcgg tttggcgcat aacagcctgc actggctgtc gccgctgatg
    15721 gcgttgctgc ttttattgcg cctgcgccag ccccgtcgtc aggcagggcc gctacaagcc
    15781 gtcacaaaaa tcgtcgctgt tgtgggcatt gtattgtgtg tctccagttt tctgttgaaa
    15841 acgcaccagt tactgctgtt ttacccggta gtggttaacg ctgtcatgct ggcggttttt
    15901 ggcggttcgc tgcggtcgac aatgccgata gttgagcggc tggcacgttt gcaggaacca
    15961 gacctcccgg aaaaggccgt gcgatatacc cgccgcgtaa cgcaggtctg gtgcctgttt
    16021 tttattttca acggcagtat tgcgttattt accaccttgt atggcaacat gcctttgtgg
    16081 acagcctgga acggtatcat tgcttacctt ctgataggtt tcctgactgc aggggaatgg
    16141 ctgatacgtt gtcagatgat caaacggaaa acaccatgaa tcagactctc tcactttcac
    16201 agtggttgac agcctctcgc cctgtaacaa ctcctgttgc ctggcttggg gaatatacct
    16261 ggacactggg gcatctgcgg catgatgtag cgcttttgat cgaccattta cgcgaccagc
    16321 caggcaatcg ctgggcactt tgttttgaaa acagctatct ttttattgta gcgttgttgg
    16381 ccacgctaca ctcaggtaaa atcccagtaa tcccgggcca taatcgcgcc gctttgttaa
    16441 atgtacaacg ctccttgttt gattgcatcc tgagtgataa aaggctgggc tggaacggtc
    16501 cacagtttgt tgtaagcaca tctcaccaga tgacgaccag cacgatagcc tttgatgata
    16561 ttgcttctga cgcgtttatt gaacttttca catcgggttc aaccggacaa ccaaaacaaa
    16621 ttgcaaaacc tgttatcagc cttgatcaag aagcaaacat gctggcaact cactttgcag
    16681 accgactact gggctgtcgt tttgtagcat ccgtgatgcc tcagcacctg tatgggctga
    16741 ctttccgtat ttttttacct atggcacttg gcctgccatt gcatgccgca atgctctggt
    16801 attctgagca acttgccgca ttaagccata cctaccgcta tgcctttgtc agcagcccgg
    16861 cgttccttaa acgactcgac cttcatctga cgcctccccc cgtggaaatg attctctctg
    16921 ccggcggtat gctaccatgg caggatgtag agaaaacttc cacctggctg aatatctggc
    16981 ccgatgaaat ctatggtagt accgaaacag gtattctcgc atggcgttac cgtcaacagg
    17041 ataatatccc ctggtttact ttttctgatg ttcatctttc acaggaaagt gagggggttc
    17101 gtgtcttttc gccactcatc cctgctgaag gattggtgct tgacgatatg ttgcaattca
    17161 atgaaaacgg tcagttccac ctgatcggac gacgtggtcg agtggtaaaa atagaggaga
    17221 agcggatatc actggttgaa atcgaacaac ggcttctggc acttgacggt attatggatg
    17281 tcgctgcaat accattaata cgcaatggcc gccaggctat cggtgtcgtg gttgtaccaa
    17341 ataaagaggc ccgtcaaata tggcaatgct ccggagggaa aactctggag ttgtcctggc
    17401 gcaaagcact attaccatgg cttgaacctg ttgcagtacc tcgttactgg cgcattattg
    17461 atgaaatacc agtaaacaat atgaataagc gtgtctatgc acaattacag gagttatttc
    17521 atgatacgcc atgaaattga gcgccatcag gttcaactgc aggagatgga aatcgttcta
    17581 taccttgacc cgatgctcta ctggtttaat ggtcattttg ccgtacagcc tctgttacca
    17641 ggcgtggccc agttggactg ggtaatgcat tatgctacca cgctgcttgc tccgggctgg
    17701 cgttttcgta gtatccagaa tgttaaattt ctggcgccac ttatacctga gacgactgtt
    17761 accctgcaat taacatggca ggaaacaagt caggtactca ctttctgcta tcagcgtcac
    17821 gacggtgacg cccgccacac cgccagtagc gggaagatcc gcctatgtcg gtaaactttt
    17881 ctccctgcgt gttgatcccc tgctacaacc acggtgcgat gatgccgggc gtgctggcgc
    17941 gtcttaagcc atttaatctt ccctgtattg tggtggatga cggcagcgat gccgccacgc
    18001 aacagcaact ggataatctg gttgccgaac agcctggcgt gaccttaatt cgcctggcag
    18061 aaaacacagg caaaggcgcg gcggtaatgc gcggcttaca ggcggcggca gacgcgggct
    18121 tcagccatgc ggtgcaggtg gatgctgacg gtcaacatgc gattgaagat atccccaaac
    18181 tgctggcact cgctgaacaa caccctgcgg cgctgatctc cggccagcca atttacgatg
    18241 actccatccc ccgctcacgg ctttacgggc gctgggtcac ccacgtctgg gtatggatcg
    18301 aaacgctctc cctgcaactg aaagacagta tgtgcggctt tcgcgtttat ccggttgcgc
    18361 caacgctgca actggcaaaa tacgccacca tcggcaagcg gatggatttc gacactgaag
    18421 tgatggtgcg cctctactgg cagggaaaca cctgttattt cgtgccaacc cgcgtcactt
    18481 atccgcagga cgggctttcg cattttgatg ccctgaaaga taacgtccgc atctcgctca
    18541 tgcacacgcg tctgtttttc ggcatgttgc cgcgtattcc ttcactactg atgcgccgct
    18601 cttccagcca ctgggcgcgg cagagtgaag tgaaaggatt atggggaatg cgcctgatgc
    18661 tgctggtctg gcgtctgctg ggaagaacgg cgtttagcgc gctgctttac ccggtggtgg
    18721 gcgtctactg gctcactgct gctcgtgcgc gcaaagcgtc gcaagactgg ctcgcccgtg
    18781 tacgacagca tcaaccacag gcggcaaaac tcaacagcta tcagcacttt ctacgtttcg
    18841 gtaatgccat gctcgacaaa atcgccagct ggcgcggcga gctacaactg gggcacgatg
    18901 tgctgtttgc gccaggcgca gaagcggcgc ttgacgtccg cgatccgcgc ggcaaattgc
    18961 tgctggcctc gcatcttggc gatgtggaag tgtgccgggc gctggcaaaa attcagggct
    19021 acaaaaccat taacgcgctg gtgtttagcg aaaacgccca acgctttaaa cagataatgc
    19081 aggagatggc tccccaggca ggcattaacc tgatgccggt aacagatatc ggcccggaaa
    19141 ccgccatcct gctgaaagag aagctggata acggcgaatg ggtggcgatt gtcggtgacc
    19201 gcatcgccgt caccccgcaa cgcggcggcg actggcgcgt ctgctggagt ccgtttatgg
    19261 gccagcctgc gcctttccca caggggccgt ttattctcgc gtctattttg cgctgcccgg
    19321 tgaatctgat tttcgccctg cgccagcgcg gcaagctgca tattcactgc gaaacctttg
    19381 ccgacccgct gctgttgccg cgtggcgaac gccaacaggg gctgcaaaac gctatcgacc
    19441 actacgccgc gcgtctggaa cattacgcgc tccagtcgcc cctcgactgg tttaattttt
    19501 tcgatttctg gcaactgccg gaaattcagg acaaggagta aagggtgctt aacgatcccc
    19561 gatttaccgc tgaagtcgag ctgaccattc cctttcacga cgtcgatatg atgggcgtgg
    19621 cctggcacgg taactatttt cgctactttg aagtggcccg cgaggcgctg ctcaaccagt
    19681 tcaattacgg ctatcggcag atgaaagagt caggttattt gtggccagtg gtcgatgcgc
    19741 gggtgaaata tcgccacgcc ctcacctttg agcaacgcat ccgcgtgcgt gcgcatatcg
    19801 aagagttcga aaaccgtctg cggattggct atcagatttt cgatgccgaa acgggcaaac
    19861 gcgccaccac aggatacacc attcaggtcg cagtagagga gcaaagccgt gaactgtgct
    19921 ttgtcagccc cgatattctg tttgaacgca tgggagtcaa accatgaaat ttttaccgct
    19981 gctggcgctg ctgattagcc cgtttgtgag cgccctgacc ctggacgatc ttcagcaacg
    20041 ctttaccgaa caaccggtga tccgcgccca tttcgatcaa acccggacga ttaaagatct
    20101 gccgcagccg ctgcgatctc agggtcagat gttgatcgcc cgcgaccagg ggctgttgtg
    20161 ggatcaaact tcaccattcc ccatgcagct attgctcgac gataaacgca tggtgcaggt
    20221 gatcaacggc cagccgccgc aaatcatcac ggcagaaaat aacccgcaga tgttccagtt
    20281 taaccacctg ctgcgcgcgc tgttccaggc cgatcgcaaa gtgctggaac aaaacttccg
    20341 cgtcgaattt gccgataaag gcgaaggccg ctggacgctg cgcctgacgc cgaccaccac
    20401 gccgctggat aaaattttca acaccatcga tctcgccggg caaacctatc tggagagcat
    20461 tcagcttaat gataaacagg gcgatcgcac cgatatcgct cttacccaac atcaactgac
    20521 gccagcgcaa ctgagcgatg acgaacgcca acgttttgcc gcccagtaaa cgccccgcgc
    20581 tgttatgggg gctagtctgc ctggtcatgg cggtggtatt gctgatcctg ctgccgcaat
    20641 cacggctgaa cagtagcgtg ctggctatgt tacccaaaca ggcgatgggc gatattcccc
    20701 cagcgctgaa tgacggcttt atgcagcgtc tggaccgcca actggtgtgg ctggtcagcc
    20761 ccggtaaaga ggctaatccc agggtggctc aggaatggct gacgctgctg caaaagtccg
    20821 ctgcgcttgg cgacattaaa gggccaatgg atgccgccag ccagcaggcg tggggggcgt
    20881 ttttctggca gcaccgcaac ggcctgattg accccgacac ccgcgcccgc ctgcaaaacg
    20941 gcggcgaagc gcaggcacaa tggatcctct cccagcttta ttctgcgttc tccggggtaa
    21001 gcggcaagga gttgcaaaac gatccgctga tgttaatgcg cggctcgcag ctggcgatgg
    21061 cgaaaaacgg ccagcgttta cgactgatgg acggctggct ggtgacgcag gatccccagg
    21121 gcaactactg gtatctgctg cacggcgaac tggcgggatc gtcgtttgat atgcagcaaa
    21181 cccaccagct catcacgacc ctgaatacgc tggaaaaaga tctgaaaacg cgttacccgc
    21241 aggcgcagtt gctctcgcgc ggcacggtgt tttacagcga ttacgccagc caacaggcga
    21301 agcaggatat ctccaccctg ggcgtggcaa cgctgctggg ggtgatattg atgattgtgg
    21361 cggtgttccg ctctttacgc ccgttgctgc tttgcgtgat ttccatcggc atcggcgcgc
    21421 tggcgggaac ggccgccaca ttgttgattt tcggcgaatt acacctgatg acgctggtga
    21481 tgagcatgag cgttatcggc atttccgctg actacacgct ctattacctc accgaacgga
    21541 tggtgcacgg caacgacgtt tcgccgtggc aaagcctggc gaaagtgcgc aatgccttgc
    21601 tactggcgct gctcaccacc gtggcggcat atctgattat gatgctcgcc ccttttcccg
    21661 gtattcggca aatggcgatt tttgccgccg tcgggttgag cgcctcctgt ctgaccgtcc
    21721 tgttctggca tccgtggctg tgccgtggcc tgtcggtacg cccggttccg gcgatggcgc
    21781 tgatgctgcg ctggctggcg gcgtggcggc gtaataaaaa actgtcgctg ggtctgcccg
    21841 tcgcgctggc gctgttttcg ctggcgggga tgtcaatgct acgcgtcgat gacgatatct
    21901 cgcagttaca ggcgctgccg cagcatattc tggcgcagga gaaagccatt accgccctga
    21961 ccggacaaag cgtcgatcaa aaatggtttg tagtttacgg cgactcccca cagcaaacct
    22021 tgggacggct ggagacattt accacctcgc tggagaaagc gaaaaaagac ggccttatca
    22081 gcaactaccg caccattccg ctgaactccc tggcgcggca ggaggaagat ttagacctgc
    22141 tgaaaacggc tgccccgacg gtaacaaaag cactgcaaaa tgccgggctg acggcggtga
    22201 acccggatct taacgccatg ccagtgaagg ttgatgaatg gctggcaagc cccgccagtg
    22261 aaggctggcg tctgctgtgg ctgacgctgg aaaacggcga aagcggcgta ctggtgccgg
    22321 ttgaaggggt taaaagtagc gcgttgcagc aggaaatcgc cagctattac cctggcggca
    22381 ttgcctgggt tgatcgcaaa agcacctttg atgaattgtt cgcactttac cgttatgtgt
    22441 taaccggctt gttgctggtg gcgctggcag taattgcctg cggcgcagtg gcccgcctcg
    22501 gctggcgcaa agggcttatc agcctggtgc cttcggtgct ttcgctgggc tgcggtctgg
    22561 cagtgctggc gatgagcggg caggcggtga atctcttttc gctactggcg ctggtgctgg
    22621 tgcttggcat cggcatcaat tacacgctgt ttttcagtaa tccgcgcggt acaccgttaa
    22681 cttccctact ggcgatcacg ctggcaatgc tcaccacctt gctgacgctg ggaatgctgg
    22741 tattcagcgc cacccaggcc atcagcagtt ttggtattgt gctggtgagc ggtattttca
    22801 ccgccttcct gctttcgccg ctggctatgc ccgataaaaa gagaacaaaa aaatgatcaa
    22861 atccaccttc tggcgagcgc tcgccctgac cgctacgctt atcctcaccg gctgtagcca
    22921 ctcgcaaccg gaacaggaag gccgcccgca ggcgtggctg caacctggca cacgcatcac
    22981 gctgcctgcg ccggggattt cacccgccgt caattcccag caactcttga gcggcagctt
    23041 caacggcaaa acccagtcgc tgctagtgat gcttaacgcc gacgaccaga aaatcaccct
    23101 tgccgggctg tcgtcggtcg gcattcgcct gtttctggtg acctacgatg caaaaggtct
    23161 acgcgccgag caatccatcg tcgtcccgca gctgccgccc gcaagtcagg tgctggctga
    23221 cgtaatgctc agccactggc cgattagcgc ctggcaaccg caacttccca ctggctggac
    23281 gcttcgcgac aacggcaaca aacgcgagct gcgtaacgcc agcggcaagc tggtcacgga
    23341 aatcatctat ctgaatcgcc agggcaaacg cgtgccaatc agcattgagc agcatgtctt
    23401 taaataccac atcaccattc aatacttagg tgactgatat gatttatatt tccgccgtcg
    23461 gcatgatcaa cgccctggga aataatcttg acgaaatcgc agccaacctg gctcgcggtg
    23521 tcgcccccgg catgcgtccg cgcgcaggct ggttgcaggg gcatccgcag gcggtcctgg
    23581 ctggcgtgga tggcgagctt ccgcttatcc ccgaaaaatt cgctgcgcac cgctcgcgca
    23641 acaatcagat cctgctggcg gcgctggcgc agatccagcc gcaggttgat gacgctatcg
    23701 cgaaatatgg ccgcgagcgc atcgcgattg tgcttggcac cagcacctcc ggcctgcatg
    23761 agggcgacac gcacgttaac ctgcgcacca acggacagcc gagcaccaca tggcactacg
    23821 cgcaacagga actcggcgat ccgtcgcgtt ttctcagcca ctggctggcg ctcgacggcc
    23881 cggcctatac cctttcaacc gcctgctctt ccagcgccag agcgatgatc agcgggcgca
    23941 ggcttatcga agcggggctg gtcgatgccg ccattgtggg gggcgcagat actctgagcc
    24001 ggatgccgat taacggtttt cacagcctcg aatcgctctc gccaacctta tgtcagccgt
    24061 ttggtcgtga ccgcgcaggt atcaccattg gcgaaggggc gggactgatg ttattaaccc
    24121 gtgaaccgca gcccatcgcg ttactgggcg ttggtgaatc gagcgacgct taccatattt
    24181 cggccccgca tccgcaaggc gaaggggcga tccgcgccat taaccaggcg ctgaccgatg
    24241 cgcagcttac gccagatgac atcggctata tcaacctgca cggtaccgcc acacaactca
    24301 acgaccagat tgaatcaatg gtggttaacg ccctgtttgg caaacgcata ccgtgcagct
    24361 ccaccaaaca tttgaccgga cacacgctcg gcgcggctgg cattaccgag gcggcaatta
    24421 gtatgttgat tttacagcgt gatttaccgc tgcccgctca ggacttcagc ctgtcgccgc
    24481 gcgatcccac gctgccaccg tgtggcatta tcgaaaaacc gcagccgctg gcacgtccgg
    24541 tgatcctgtc caattccttc gcctttggcg gcaataacgc cagcattctg ctcgggaggg
    24601 tttcgtgagc cactatttat cccccggcgc ttatctgccc cacgacgcgc ccatgttact
    24661 gctggaagac gtggtgagcg tcagcgatga cagcgcggtg tgccgcgtga cggtttcgcc
    24721 cagtggcgta ctggcaccgt ttctcgaccc ggacggcaat ctccctggct ggtttgccct
    24781 tgaactgatg gcgcaaaccg tcggcgtctg gtctggctgg catcgccacc agcagggcaa
    24841 aaacagtatt gagttaggga tgatactggg cgcgcgtgaa ttgctttgca ccactggcat
    24901 tctgcccgca ggccagacgc tgaccattac cgtcaaactg ctgatgcagg acgatcgctt
    24961 cggcagcttt gagtgcagca tcaacgacgg cgaggcgacg ggccgcatta acaccttcca
    25021 gccgaccgcg gaagaactta ccaccctgtt tcaacaagga gcgtcctgat gagtcgttca
    25081 gttctggtta ctggtgccag caaaggcatt ggtcgcgcca tcgcctgcaa gcttgcggca
    25141 gacggcttta acattggcgt tcactatcac cgtgacgcca caggcgctca ggaaacgctg
    25201 aatgccattg tcgccaacgg gggtaacggg cgtttgctca gtttcgacgt cgcaaaccgc
    25261 gaacagtgtc gggaagtgct ggagcatgag atcgcgcaac acggtgcctg gtacggcgtg
    25321 gtcagtaacg ccgggatcgc ccgcgatgcc gcttttccgg ctttaagcga tgacgactgg
    25381 gatgcggtga tccacaccaa cctcgacagt ttctacaacg tcattcagcc gtgcattatg
    25441 ccaatgatcg gcgcgcgcca gggtgggcgc atcatcacgc tgtcctcggt ttccggcgta
    25501 atgggcaatc gcggccaggt gaattacagc gcagccaaag ccggaattat cggcgcaaca
    25561 aaagcactgg ctatcgaact cgcaaagcgc aaaatcaccg tcaattgcat cgcgccaggg
    25621 cttattgata ccgggatgat cgaaatggaa gaaagcgctc tgaaagaggc aatgtcgatg
    25681 atccccatga aacgtatggg ccaggcggaa gaagttgccg ggcttgccag ttatttaatg
    25741 tctgatattg cgggttacgt cacccgccag gttatttcca tcaacggagg gatgttatga
    25801 cacgtcgcgt agtgattaca ggaatgggcg gcgtgactgc ctttggtgaa aactggcagg
    25861 acgtttctgc acgcttactg gcgtatgaaa atgcggtgcg caaaatgccg gagtggcagg
    25921 tctacgatgg gctgcacacc ctgcttggcg cgcctgtgga tgatttcacg ctaccggagc
    25981 actacacccg caagcgtatc cgtgcgatgg gccgcgtgtc gcaaatgtcg acccgtgcca
    26041 gcgagctggc gttagagcag gcagggttga ttggcgatcc gatattaacc agcggcgaaa
    26101 cgggcattgc gtacggttca tcgaccggca gtaccggtcc ggtgagcgaa tttgccacca
    26161 tgctgactga aaagcacacg aacaacatca ccggcaccac ctatgtgcag atgatgccgc
    26221 acaccaccgc tgttaatact ggcctctttt tcggcctgcg cgggcgggtg atcccaacct
    26281 ccagcgcctg tacctccggc agccaggcga tcggttatgc gtgggaagcc attcgccacg
    26341 gttatcaaac ggtgatggtt gccggtggtg ccgaagagtt atgcccttca gaagcagcgg
    26401 tgtttgatac cctttttgcc accagccagc acaatgatgc gccgaaaacc acaccttcac
    26461 cgttcgatga aaaccgcgac ggactggtga ttggtgaagg cgcaggtacg ctcattctcg
    26521 aagaactgga gcatgccaaa gcgcgcagcg cgaccattta cggtgaaatc gtcggctttg
    26581 cgactaactg cgacgcggca catattaccc agccgcagcg cgaaaccatg cagtattgta
    26641 tggagcaatc gctaaaaatc gcagggttaa gcgcacaaga tatcggctat atttcagcgc
    26701 atggtacggc gaccgatcgc ggtgatatgg cagaaagtct ggcgacagca accatttacg
    26761 gtgataatgt gccgctctcc tcgctgaaaa gttattttgg tcatacgctt ggtgcctgtg
    26821 gcgcgctcga agcctggatg agtctgcaaa tgatgcgcga aggttggttt gcgcctacgc
    26881 taaacctgaa taaaccagac ccgaattgtg gcgcgctgga ttacatcatg catgaagccc
    26941 gcaaggttga ttgtgagttt ttgcagagta acaactttgc ctttggcggc attaatacat
    27001 ccatcattat taagcgctgg ccctgagtta tacaaccaca tggacatccc atgtggtgat
    27061 tcttcaaaaa atctgaatca atgaatatga acctcaaatt ctcttacgca tactgaagag
    27121 cattccgtat aacgattcag cagacaaaac tttatcagtc cacacgaatc aacggatgca
    27181 cagatgacac gcccggttat caccgggcaa tggttgccat aacgggtaac ataagggact
    27241 gaacagttgt ctttagcgca acgaactgat ctaacgtacc tgaacggaca acctgaaaac
    27301 agccttccat cctgcattgt ccgccagtgc ttattaccaa ccacgtctgt tgcagtaaag
    27361 taatcagttg cgaacattat ttatcaatcg tgacgctggc tcgcgcatgg cagaaaaggt
    27421 cctgactgag caacaacagg catcgtactg ttgctgctcc atgttgttta tatgaacgca
    27481 tcccgtttgc aaggcttccg tgtttttgac gttgacggta gattgctgct ctatatcctt
    27541 attgcagccg gtgctgcggg cctcaatgag attcgctgac tctcgcctta ttcacgcaac
    27601 gagctttaag ctcagaaaaa catccaagtt tggtgagtca cagtcttacc tgtcgttgtc
    27661 atcaatatag gaatggataa tctggagagc gatactgcag gctgatattg cggttttgcg
    27721 acaagttttt agcatccgct tctacgccaa atacctgaag taggaatgga tgcgttagca
    27781 gcatacatcc ggaactgcgc ggggaatagc cggaaacggc tatccctacc acaatagcta
    27841 actttgtaat agcgctcttt taggctcatg tactctgacg taattctatc tggtagttga
    27901 attgcacgat acgcgactct tctgattcat catgattatt attcaatagt ccaatcagta
    27961 acttacctgt ctctatcccc atttttttgt agtcaatacg gatggatgtc aaagttgggt
    28021 gtgtttgatc gcaattgtct gaaccgtcaa gacaagctac agcgatattt ccagggattt
    28081 ttaacaaacg ccgctggcac tcaaacatta tacccagcgc aatctcttcg tggctgcaaa
    28141 tcacagcatc cagctctggt tgtcgcagta atatctcgtt taatgcgtaa cgccccaatt
    28201 gcagacttgc ggcatccggc atggtgataa caagatcagt gttctggtaa tggctcagca
    28261 ttgctttatg ccagccgttc agttgctgac gttgcaggcg gttgtccata tgcgcaccga
    28321 taaatccgat attcttataa ccctgctcca gcagatgttc ggtcagttta tgtgatgctt
    28381 caaaaaatgc tgtttcgatg gtaatttgag ccggactgaa aggggaaccg ataacgttaa
    28441 tggtaggtat atttgttttc tcgagtatct ggtgagtttt atcagccaac tgagaaccaa
    28501 aaataactaa cgccgccggg ttgccttgaa gaagcgtcat cagaatttca gcttctttga
    28561 gctgattata ctcatgacac ccaagcagta gctggaattc atttttatta agcacctgct
    28621 gtaatgactg cataaagcgg gagctggctt tatctgtcag ggaaggaacc agtacggcga
    28681 ctgtatgact gtgaccagat gcaagggcgc cagcagttct gttgggaata taacccaact
    28741 cttctacggc ctgttctatc ttttccctca gcttgtcaga aacc
//
LOCUS       DAZKJR010000057        28637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKJR010000057 DAZKJR010000000
VERSION     DAZKJR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.057"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHOWS_002485"
     CDS             complement(<1..593)
                     /locus_tag="ACHOWS_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0048040.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHOWS_002486"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHOWS_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0048041.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHOWS_002487"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHOWS_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0048042.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHOWS_002488"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHOWS_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0048043.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHOWS_002489"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHOWS_002489"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFQ0048044.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLAGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHOWS_002490"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHOWS_002490"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFQ0048045.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHOWS_002491"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHOWS_002491"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFQ0048046.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRCWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHOWS_002492"
     CDS             complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHOWS_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0048047.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHOWS_002493"
     CDS             complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHOWS_002493"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0048048.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6904..7122
                     /gene="slyX"
                     /locus_tag="ACHOWS_002494"
     CDS             6904..7122
                     /gene="slyX"
                     /locus_tag="ACHOWS_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFQ0048049.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHOWS_002495"
     CDS             complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHOWS_002495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0048050.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHOWS_002496"
     CDS             complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHOWS_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFQ0048051.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHOWS_002497"
     CDS             complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHOWS_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFQ0048052.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVTVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHOWS_002498"
     CDS             complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHOWS_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFQ0048053.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10553..12466
                     /gene="yheS"
                     /locus_tag="ACHOWS_002499"
     CDS             10553..12466
                     /gene="yheS"
                     /locus_tag="ACHOWS_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0048054.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12466..13488
                     /gene="yheT"
                     /locus_tag="ACHOWS_002500"
     CDS             12466..13488
                     /gene="yheT"
                     /locus_tag="ACHOWS_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0048055.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13482..13700
                     /gene="yheU"
                     /locus_tag="ACHOWS_002501"
     CDS             13482..13700
                     /gene="yheU"
                     /locus_tag="ACHOWS_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFQ0048056.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13754..14623
                     /gene="prkB"
                     /locus_tag="ACHOWS_002502"
     CDS             13754..14623
                     /gene="prkB"
                     /locus_tag="ACHOWS_002502"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFQ0048057.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHOWS_002503"
     CDS             complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHOWS_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFQ0048058.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15384..16016
                     /gene="crp"
                     /locus_tag="ACHOWS_002504"
     CDS             15384..16016
                     /gene="crp"
                     /locus_tag="ACHOWS_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFQ0048059.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHOWS_002505"
     CDS             16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHOWS_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFQ0048060.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHOWS_002506"
                     /gene_synonym="dapC"
     CDS             complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHOWS_002506"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFQ0048061.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHOWS_002507"
     CDS             complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHOWS_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFQ0048062.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHOWS_002508"
     CDS             complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHOWS_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFQ0048063.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHOWS_002509"
     CDS             complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHOWS_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFQ0048064.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHOWS_002510"
                     /gene_synonym="rotA"
     CDS             complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHOWS_002510"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFQ0048065.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHOWS_002511"
     CDS             21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHOWS_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFQ0048066.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILIV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23275..25818
                     /gene="nirB"
                     /locus_tag="ACHOWS_002512"
     CDS             23275..25818
                     /gene="nirB"
                     /locus_tag="ACHOWS_002512"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFQ0048067.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHANLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25815..26141
                     /gene="nirD"
                     /locus_tag="ACHOWS_002513"
     CDS             25815..26141
                     /gene="nirD"
                     /locus_tag="ACHOWS_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFQ0048068.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26268..27074
                     /gene="nirC"
                     /locus_tag="ACHOWS_002514"
     CDS             26268..27074
                     /gene="nirC"
                     /locus_tag="ACHOWS_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFQ0048069.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSIRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27093..28466
                     /gene="cysG"
                     /locus_tag="ACHOWS_002515"
     CDS             27093..28466
                     /gene="cysG"
                     /locus_tag="ACHOWS_002515"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFQ0048070.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGQVVGLRDK
                     LNWFSNH"
BASE COUNT         6942 a         7698 c         7226 g         6771 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtca
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacgatcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatcg attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttaatctc
     2041 ttcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag ccaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataatagca gttcgtcgcc ctcacttagc aggcgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc gatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggcacagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgg ctgaaggttt
     4681 gacgaagcca gccccagtct tccggcctcc gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagcagcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgataaaaaa agacgctgct taactcatgg ccttctgcaa tcagcgcctg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcatcttta atatcgaaga
     5101 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcaaacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcggt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5881 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5941 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     6001 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     6061 ttcttcagac cttctgtcca acccggaata acaccgtcca gacggaaaga gagcggttca
     6121 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcacg
     6181 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttcgactac ctgataaacc
     6241 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6301 cctttcgctt cgttatctgc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6361 cgagcttcga atgcttgcag tgtctgttcg atctcttggt cggagagttt gctcttatca
     6421 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6481 tcttgttctt taagagaatt ttccatgtaa cgacccagcg aagcacccag tgcataagct
     6541 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6601 gcttcagcag caaaagtgat cggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6661 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6721 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6781 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6841 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6901 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6961 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     7021 aaactgcgcg atcatctgcg tctgctgacc gagaagctaa aagccagcca gccgtcgaac
     7081 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     7141 attccccgct tttttgtcac tttttcggta ttagtggcaa ccgcaaccgc cgttaccttt
     7201 accgccgcag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccaccgca
     7261 gcaaccgtcg tggtcgtgat cgtggtgatg atcgtgcgca ccgtgaacgt ggccatgagc
     7321 cagttcttct tcagtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7381 accggccagc atgtggttac catcaaccac gacgtgatcg tcttcaaccg cagtgatttc
     7441 aaccggtacc ggaccctggt cggtttcagc caggaaacgc ataccaacct gcagttcatc
     7501 aacgcccata aatacgtctt taggaacgcg ttgcaccagg ttttcgtcgt actgaccgta
     7561 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcgtgac cttccagcgc
     7621 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7681 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7741 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7801 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7861 ccggatgaaa aatcccgatc acttgctcat ctttgcgaac gtgatcgcgg gcttctttgt
     7921 ccgcttctcg catctgatgt ccgcacttaa cacattcaac aatatcaata ttattttcgc
     7981 gccacatcgc cattgaatcc tgcgcctgac aggccgggca ttttgccccc gcaataaaac
     8041 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     8101 gttcttgttg catttcacgc tggaaaatct cttccagttc gcgtcgggct tccctggcgc
     8161 gagaaatttg tacggtgtcg gcatgcattg ggatgagctc ccgcaacatc cgcatatcca
     8221 ggcggcgaaa atgcagttgc gcccgctgcg cctgatgtgg atgcatgcct agcgtgacca
     8281 gcgtcttgcg ccccagctct aacgcactgg agaatgtctc acgagaaaac tgcgtcaccc
     8341 ctgcctgtaa taactcatgc gcctccacac gtccgcgcgc tcgcgcaaga atatgtaaat
     8401 gcggaaagtg ctgctggcat atttccacca gtttcatggt gtcttccggc tcattacagg
     8461 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8521 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggcg ctgatatccc
     8581 gctccagcac agtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8641 aacgcccgaa gcccacgaca atgacctgcg gtttatcatc gttgacccac ggtttttcgt
     8701 cttcttcttc cggtccgtta aactggcggg atagccattt atccaccagc ttcatcagca
     8761 acggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8821 gaaacaaccg ttgcgaagaa gcggtagaaa agagcacaaa ggcaaactca ccgccctgac
     8881 tcaacacgcc agcaaactgc atccgctctg agctacgcac gccatataat cgcgccagca
     8941 gatacagcac gagaattttc accgtcacca gcacaaccac gcttatcact acccacaaca
     9001 gatgggtata aagcaccccg agattgagcg acatgccgac agagataaag aacaaaccga
     9061 gcagcaagcc tttgaaggga tcgatagctg tttccagttc gtggcgatat tcgctttctg
     9121 ccagcaacac gcccgcaata aacgtaccga gcgccatcga cagccccagc gcatccataa
     9181 acaatgcgga acccaacacc agcagcagcg tcgcggcggt gaacacttcc cgcacgccag
     9241 aagctgcaat aaaacggaat accggacgca gtaaatagcg cccgccaatc agcatgccga
     9301 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9361 ccaacaacgg cactaacgcc agcgccggga tcactgccag atcctgaaac agcagaaccg
     9421 aaaaacctag ctgcccggat tcgctgcgat tcatcccttt ctcacgcatc agttgcaacg
     9481 ccattgcggt tgaggacatc gcaaggccaa taccaccgac caccgccgcc tgccaggcga
     9541 aatccgtcag catcaataat cccgccagca acgccgcgct taacagcacc tgtgccgcac
     9601 ctacgccaaa aatcgaacgc cgcaattgcc aaagcttgga gggattcaac tccaggccga
     9661 tgataaacat caggaacacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9721 taataaaacc cagcccccac gggccaattg caatccctgc cagcaaatat cccaacacag
     9781 cgccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacaaca
     9841 ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9901 cagccagtca ccgtaggctc tggcgtgact cgccagctcc tgtgcgcttt gccgtctcgc
     9961 ccagtaaata atgatgggac tcaaccaatg catccggcac atgcccgccg ctagttcaaa
    10021 ggggcgcaga acatcgctca tcgggtagcg attcaaggcg tcataacggt aagcactttc
    10081 cggctcgccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttcctcccgg
    10141 cccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta gcgccgggca
    10201 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
    10261 ctcacggggg atatcaataa aaaaatcggg atagtgcgcg taaaggtcgt gcacggtaac
    10321 attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc
    10381 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10441 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10501 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10561 tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10621 catcaacccc gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatcgaccct
    10681 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10741 ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg cgctggaata
    10801 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctacacg acgccaacga
    10861 acgtaacgac gggcacgcca tcgccactat tcatggcaag ctggatgcta tcgacgcatg
    10921 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10981 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    11041 gctgatttgc cgttcagact tgctgctgct cgatgaaccg actaaccacc tcgatctcga
    11101 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    11161 tcacgaccgc gacttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    11221 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    11281 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcgcatctgc aaagttatat
    11341 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgta ttaagatgct
    11401 ggagcgtatg gagctgattg ccccggcgca cgttgacaac ccgtttcgtt ttagcttccg
    11461 cgcgccggaa agcctgccta atccgttatt aaagatggaa aaagtcagcg caggctatgg
    11521 tgatcgcatt attctcgact cgattaaact gaatctggtc cccggctcgc gcattggtct
    11581 gctaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gcgagcttgc
    11641 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11701 tcaactggaa tacctgcgcg ccgacgaatc gcctattcaa catctggcac gtttagcgcc
    11761 gcaggaactg gagcaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11821 agtgacggaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11881 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgactaacc accttgacct
    11941 cgacatgcgt caggcactga ctgaagcatt aatcgagttc gaaggcgcgc tggttgtcgt
    12001 ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    12061 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca
    12121 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcaaacagcg cccaggcacg
    12181 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccaaccgc tgcgtaaaga
    12241 gatcgcccgt ctggaaaaag agatggaaaa gctgaacgca cagctggcgc aggcggaaga
    12301 gaaactcggc gacagcgaac tgtacgatca aagccgtaaa gcggagttga ccgcctgcct
    12361 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcgt ggctggaagc
    12421 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataacg
    12481 acgaccgatg ccaatgaatt cagcagcagt gctgaattca cccctatgcg cggctttagc
    12541 aattgtcatc tacaaaccat gctgccgcgt ctgtttcgtc gcaaggtgaa attcaccccg
    12601 cactggcagc gactggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagat
    12661 cctgcacagg cgcggcataa accgcgttta gtcgtgtttc acgggctcga aggcagtctc
    12721 aatagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12781 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12841 ggtgaaaccg aagacgccag ctggttttta cgctggctgc agcgcgaatt tggacatgcg
    12901 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tcgcctgttt gctggcaaaa
    12961 gaaggtaata acctcccgat tgatgcagcg gtaattgtct ccgcgccatt tatgctggaa
    13021 gcctgtagtt atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    13081 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacact gccgattaat
    13141 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctaat caccgccaga
    13201 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctc
    13261 aatcggatcg ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat
    13321 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact gactgaacat
    13381 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13441 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13501 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13561 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13621 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat atcatgccgc
    13681 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13741 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13801 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13861 agccgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat
    13921 ccgcaaagca cgcgacgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt
    13981 cggcctgctg gaacaaacct tcattgaata tggtcagagc ggcaaaggga aatctcgcaa
    14041 atatctgcat acctacgatg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    14101 ctggcaacct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    14161 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    14221 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    14281 agaagcagtg atggactcgg tagtgcgttc aatggaagac tatatcaact acatcacacc
    14341 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc
    14401 gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa
    14461 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14521 catcaatacg ttagtggtac cgggcggcaa aatgggtctg gcaatggaat taattatgct
    14581 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactttgct attacgccgg
    14641 ataaaatact atccggcttc acaatgggat agttaaatca cgcggcaacc acttcatacg
    14701 agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag
    14761 agagatcaac cgcacgtgca accgctgcgt ctttcaggtc gcgaccggta acgataaaat
    14821 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14881 cttcacaatc gaccacatcc tgacgccctt tttgcaggat agaaaccaca tcgatggcac
    14941 tgcaaccccc cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt
    15001 tgccgtccat taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    15061 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    15121 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    15181 ggagacacaa agcgaaagct atgctaaaac aatcaggatg ctacagtaat acattgatgt
    15241 actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag
    15301 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15361 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15421 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15481 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15541 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15601 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15661 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15721 ttctgatgcg tctgtctgca cagatggcgc gtcgcctgca agtcacttca gagaaagtgg
    15781 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aacctggcaa
    15841 aacaaccgga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15901 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15961 atcagaatct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    16021 gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    16081 gatttatcac cccgatatta actatgcact gcgacaaacg ctggtgctat gtttgcccgt
    16141 ggccgttggg ttaatgcttg gcgaattacg attcggcctg ctcttctccc tcgttcctgc
    16201 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    16261 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag gtgctactgg caaaagatgt
    16321 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgaactggg
    16381 gccattgcac gcaaaattgc ttcccgcatc gctgctcgcc gccattttta ccctcagtct
    16441 ggcaggatac atgccggtct gggaaccgtt gctcatctat gcgttgggca ctctctggta
    16501 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact
    16561 aagtctgctg taccgcgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca
    16621 gcacaccgac cctgaaaaag cgctgccgcc gctgcttgtg cgccagcaaa aagcggtcga
    16681 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata ataccgatta
    16741 caagcggatg ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag
    16801 tttgcatcag ccggaagagg tgcaaaaact ggtcgagcgt agccatgcgg aagaagttat
    16861 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggccgatg acattcttta
    16921 tcatcgcctg ccgacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16981 ccgccagcat ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc
    17041 ccgcgtgctg cgcacccaaa aaccgctcta tgcgcgtgac ttactggccg ataaacagcg
    17101 gcgaatgccg ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cggcgctacg
    17161 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcactgca
    17221 tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacgcaaa atggctatgg
    17281 cgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    17341 gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac
    17401 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17461 tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc
    17521 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17581 gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat gacgctttag aagcctatca
    17641 ggacgcgatt cgcttgattc tgagcgagga cccgcaacct acgccactgg cctggcagcg
    17701 aatgcgggtg aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga
    17761 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17821 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17881 ggaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17941 actggagtat gacgaaccgg ggagttctgg cgatgccaat atcatggatg cgccggagat
    18001 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacaacggg ttattggtca
    18061 tctgaacacc atgcacacca tttcgtcgat ggcatggcgg cagcgaccgc atcacgggat
    18121 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggatgag
    18181 gcgttcacgc cgcatccgac atcttttgcc tgacgcgtct tatttacgcc ccaatcacct
    18241 tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcgat atccgcatct tccaccacca
    18301 gcgacggcgc aaaacgcatc acatcaggcc cggcattcag caccattacg ccttcctctg
    18361 caccggcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc tccgcgccaa
    18421 ttaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg atcttctgca
    18481 aatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga
    18541 tgatatcaaa cgccgcgccc gctactgcac aggccagagg attaccgccg taggtggaac
    18601 cgtgagaacc aggatgaaat gcagaagcaa tttccgccgt ggtcagcatg gcgctaatcg
    18661 ggaagccgcc gcctaacgct ttcgcagagg tcagaatatc cggcgtcaca ccgtagtgca
    18721 tgtaagcaaa caaatcgcca gtccgcccca tcccgcactg cacttcatca aacaccaata
    18781 atgcctgatg ttgatcgcac agctcgcgca agccctgcaa aaactctggc gtcgctgccg
    18841 tcacaccgcc ctcgccctgg atcggctcaa ccaccaccgc acaggtatgg tcatccatca
    18901 ccgctttcac tgcgtggaga tcgttaaaag gcacgtggat gatgtctgcc ggttttggcc
    18961 caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat
    19021 gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt
    19081 aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca
    19141 cgcgttcggc aaaagttgcc tcaatcagtt tacggccaag acgcagcgcc ggctcattgg
    19201 tgaaaacgtt actaatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg
    19261 caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg acatactcct
    19321 tgccctgttg atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag
    19381 cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg
    19441 ccatctcatg atcaccctgt tacgcataaa taaatatgaa attataatca caaaatatgc
    19501 ataaaaaatc actgaatggc aatcagaaat cagcgatgca gaaaattagc cagcagttga
    19561 tgtccttgtt cgctgagaat actttctgga tggaattgca caccttccag atcccactgg
    19621 cgatggcgaa tccccataat ctcgcgggtt tcgctccagg ccgtcacttc aaagcactcc
    19681 ggtaatgagt caggttccac caccagcgaa tggtagcgtg tcacggtgag tggatttgcc
    19741 agccccttaa atacgcccac accattatgt gtaatcggcg aggttttgcc gtgcatgacc
    19801 tttgcggcgc gcacaacttt gccgccaaat gcctgcgcca ttgcctgatg accgaggcag
    19861 acgccaagaa tcggcaagcg tccggcatag tgacgaataa cgtcaaggga gatcccggct
    19921 tcatctggcg tacaggggcc gggtgagatg acaatttttt gtggtttaag ggcgtcgata
    19981 tccgccaacg tcaacgcatc gttgcgctta accaacacat ccgcccccag ttcacaaaag
    20041 tactggtaga ggttccaggt aaaagaatcg tagttatcaa taagcaggat catggcggct
    20101 ccggtacgaa agaaccgcgc tattttactc agattccccg gcttcgctta ccactttgct
    20161 aaatatcatc tgcagcgcgg tgagatcccc cattgcccca ttctgatttg cctgattcca
    20221 ggcctctttt tcaataccct gccagctcag ttgatatccg gcatgaatcg ccagttgctc
    20281 gaagaagatc cgctgtgcca aaccacttcc cacccggaag ggatgcagca cgttgatttc
    20341 acaatagtaa tgcgccagcc gctcgacgaa cttcgctttc tccaggccaa ccagataacc
    20401 ttcttcctcc agatcctgca tcagggcatt gccctctttt tcgatataag caaagtggca
    20461 gaacggcgta tcgccctgat aaatatcgac ttcgcgcagt tgccccgccc agtcgaaaat
    20521 atcctgatac aactggcgat gaagagtacg caaatgcggt aaaccccgaa ccaacgggcc
    20581 aagttcaatg gtcgcagcac gcagcgccgt catttcgtaa gcggcctgtt ccagccgctg
    20641 ctgctggcgg atgttcagcc ggttacgcat gatatcaagg cctggataaa gatacggatc
    20701 gcgcccttcg ccgaatttat cgctcatagt gcctcctcag cgcttcaaga cgcgctaaag
    20761 cctctgcggc agtaagagtg actaaaggca tctcgacgcc ttcaagacgg cgactggcct
    20821 ggaaattacg attacgctga agctcccaga gacgggactt ttgcttatcg gtgagtttct
    20881 tcacttgatg cctccctgaa cgtgtccatt tgccacaagt ataagcggca aatccgggtt
    20941 acgccgggag agcagaagcg cgggcaagat tgcccgcgcg agaaatcatt acggcaggac
    21001 tttagcggaa aggataacta ccggttttga cggcacattc tggtacggac caacgtcatg
    21061 agtcggcacc tgggaaatct tatcggcaac gtccatgcct ttcaccactt taccaaatac
    21121 cgcgtaaccg aaatcacgct gaccgtggtc aaggaaggcg ttatcggcaa cgttgataaa
    21181 gaactggctg gtggcgctgt ctttgtcagc ggtacgcgcc atcgcgatgg tgccacgcgt
    21241 gttgcgcagg ccgttatcag cttcattttt gattggcggg tttggttttt tctgctgcat
    21301 ctgctcggtg aaaccgccgc cctgaatcat aaagccagga atgacgcggt gaaaggtcgt
    21361 gttgttataa aaaccactgt tcacataatc gacaaagttt tgcacagaca ctggcgcttt
    21421 ttgtttatcc agctccagtt cgatgttacc agctgaggtt gtcaacaata cgtgcgggtc
    21481 ccctttcgct gccattgctg cgggagaaag agcagaaaga gcgaaaacag cagccatcgc
    21541 cgccagggtc gatttgaaca tgagatttcc ttaacaaaca gagcagaaaa aaagcaagtg
    21601 aagagattct aaatagcctt caagaacaag gccatccctt tacctaattt tacgtatctg
    21661 aaatatatgt aacctgagga taacttttta ttccgtgatc aaaatcacct cttaaaatgc
    21721 aatttagcaa ccgattgcaa taaaacattt aatcagatca caaaatcgcc taaaatcgcc
    21781 cgctcgcggc atagcccgct atgccatttt atttttgaac gcaggccaat catgactaac
    21841 agcaatcgca tcaagctcac atggattagc tttctttcct acgcactgac cggtgcgttg
    21901 gttattgtca ccgggatggt gatgggaaat atcgccgatt atttcaatct gcctgtttcc
    21961 agcatgagta acaccttcac cttcctcaat gccggcattt taatctctat cttcctcaac
    22021 gcctggctga tggaaatcgt cccgttgaaa acgcagttac gttttggctt tctcctgatg
    22081 gtgctggcgg ttgccggttt gatgttcagc cacagcctgg cgctgttctc ggcggcgatg
    22141 ttcattctcg gggtggtcag cggcatcacc atgtcgattg gtacattcct ggtaacacaa
    22201 atgtatgaag gacgtcagcg cggttcacgc ctgttattta ccgactcctt cttcagtatg
    22261 gccgggatga ttttcccaat gatcgccgcg tttctgctgg cacgtagcat tgagtggtac
    22321 tgggtttatg cctgcatcgg gctggtgtat gttgctattt ttattctgac cttcggctgt
    22381 gaattcccgg cactgggcaa acatgcgcca aaaacagatg ctccggtaga gaaagaaaag
    22441 tgggggatcg gcgtactgtt tctctctgtt gcggcgctgt gctacatcct cggtcagtta
    22501 ggttttatct cctgggtgcc ggaatatgcc aaaggcctgg gcatgagcct gaacgacgca
    22561 ggcacgctgg taagtaactt ctggatgtcg tacatggtcg gcatgtgggc gttcagcttt
    22621 attcttcgct tctttgattt gcaacgtatc ctgatcgtac tggctggtct ggctgcgatt
    22681 ctgatgtacg tctttaacac cggaacaccg gcatatatgg cgtggtcaat tctcgccctg
    22741 ggcttcttct ccagcgcgat ctataccacc atcatcaccc tgggttcaca gcagaccaaa
    22801 gtaccgtcgc caaaactggt taactttgtc ctgacctgcg ggaccatcgg tactatgttg
    22861 acctttgtgg ttaccggccc gattgttgaa catagtggtc cgcaggcggc gctgctgacg
    22921 gcaaacggtc tgtacgctgt cgtctttgtg atgtgcttcc tgttaggttt cgtcagccgt
    22981 caccgtcagc ataacaccct gacctctcat taattgttca tgccgggcgg cactatcgtc
    23041 gtccggcctt ttcctctctt actctgctac gtacatctat ttctataaat ccgttcaatt
    23101 tgtctgtttt ttgcacaaac atgaaatgtc agacaattcc gtgacttaag aaaatttata
    23161 caaatcagca atatacccct taaggggtat ataaaggtga atttgattta catcaataag
    23221 cggggttgct gaatcgttaa ggtaggcggt aatagaaaag aaatcgaggc aaaaatgagc
    23281 aaagtcagac tcgcaattat cggtaacggt atggtcggcc atcgctttat cgaagatctt
    23341 cttgataaat ctgatgcggc caactttgat attaccgttt tctgtgaaga accgcgcatc
    23401 gcttatgacc gcgtacacct ctcgtcttac ttctctcacc acaccgccga agagctgtcg
    23461 ctggtgcgcg aaggcttcta cgagaaacac ggcatcaaag ttctggtcgg cgaacgcgct
    23521 atcaccatca accgtcagga gaaggtgatt cactccagcg ccggacgtac cgttttttat
    23581 gacaagctga tcatggcgac cggttcctac ccgtggatcc cgccaattaa aggttctgat
    23641 actcaggact gctttgtcta tcgcaccatt gaagacctca acgccattga atcctgcgct
    23701 cgtcgcagca agcgcggtgc cgttgttggt ggcggcctgt taggtctgga agctgctggc
    23761 gcgctgaaaa acttaggcat tgaaacccac gttatcgaat ttgcccctat gctgatggcg
    23821 gaacagcttg atcagatggg cggcgagcag ctgcgtcgca aaatcgaaag tatgggcgtg
    23881 cgcgttcaca ccagcaaaaa cactcttgag attgtgcagg aaggtgttga agcgcgtaaa
    23941 accatgcgtt ttgccgacgg cagcgaactg gaagtcgact ttatcgtctt ctctaccggt
    24001 atccgtccgc gcgataagct ggcaacccag tgtggtctgg acgttgctcc gcgtgggggt
    24061 atcgtcataa atgattcctg ccagacttcc gatccggata tctacgccat cggtgaatgc
    24121 gcaagctgga acaaccgtgt atttggtctg gtagcaccag gctacaaaat ggcgcaggtc
    24181 gccgttgacc atattcttgg tagcgaaaac gcctttgaag gtgcagacct tagcgccaag
    24241 ctgaaactgc tgggcgtaga cgtaggcggt attggtgatg cgcacggtcg cacacctggc
    24301 gcacgtagct acgtttacct cgacgaaagc aaagagatct acaaacgcct tattgtcagc
    24361 gaagacaaca aaaccctgct cggtgcggta ctggtgggcg ataccagcga ctacggcaac
    24421 ctgctgcaac tggtgctgaa tgctatcgag ctgccggaaa acccggattc tctgattctg
    24481 ccggcgcact cgggtagcgg taagccgtct atcggtgttg ataaactgcc ggacagcgcg
    24541 caaatctgct cctgcttcga cgtcaccaaa ggtgatctga ttgctgccat caacaaaggc
    24601 tgccacaccg ttgcggcgct gaaagctgaa accaaagcgg gtactggctg cggtggctgt
    24661 atcccgctgg tcactcaggt actgaacgcg gaactggcga aacagggcat cgaagttaac
    24721 aacaacctgt gcgaacactt tgcttattcg cgtcaggaac tgttccattt gatccgcgtt
    24781 gaaggcatta aaaccttcga agaactgctg gcgaaacacg gcaaaggcta cggttgtgaa
    24841 gtttgtaaac cgaccgtcgg ttcgctgctg gcctcctgct ggaacgaata cattctgaaa
    24901 ccggaacata ctccgctgca ggattctaac gataacttcc tcgctaacat ccagaaagac
    24961 ggcacctact cggtgatccc gcgttctccg ggcggtgaaa tcaccccgga agggctgatg
    25021 gcagtaggtc gtatcgcgcg tgaatttaat ctctacacca aaatcaccgg ctcccagcgc
    25081 ctggcgatgt ttggcgcaca gaaagacgat ctgccggaga tctggcgtca gctgattgaa
    25141 gccggcttcg aaaccggtca tgcctatgcg aaagcactgc gtatggcgaa aacctgcgtg
    25201 ggtagcacct ggtgccgcta cggcgtgggc gacagcgtcg gcctcggcgt ggaactggaa
    25261 aaccgctaca aaggtatccg gactccgcac aaaatgaagt tcggtgtctc cggctgtacc
    25321 cgtgaatgtt cagaagctca gggtaaagac gtgggtatta tcgccaccga aaaaggctgg
    25381 aacctgtatg tttgcggtaa cggcggcatg aaaccgcgtc atgcgaatct gttggcagcg
    25441 gatatcgatc gcgaaacgct gatcaaatat ctcgaccgct tcatgatgtt ctacatccgt
    25501 accgccgaca aactgacgcg taccgcgccg tggttagaaa acctcgaagg tggcatcgat
    25561 tacctgaaag cggtgatcat tgacgacaaa ctggggctga acgcacatct ggaagaagag
    25621 atggcgcgcc tgcgtgaagc ggtagtgtgt gagtggactg aaacggtcaa taccccgtct
    25681 gcgcagactc gcttcaaaca cttcatcaac agcgataagc gtgacccgaa cgtgcagatg
    25741 gtgccagagc gcgaacagca ccgtccggca acgccgtatg aacgtatccc ggtaactctg
    25801 gtggaggaca acgcatgagc cagtggaaag acatctgcaa aatcgatgac atcctgcctg
    25861 aaaccggcgt ctgcgcgctg ttaggtgacg aacaagtagc aattttccgc ccgtatcaca
    25921 gcgatcaggt gtttgcgatc agcaacatcg atccgttctt cgagtccagc gtgctgtcac
    25981 gcgggctgat tgcggaacac cagggcgagc tgtgggtcgc cagcccgctg aaaaaacagc
    26041 gttttcgctt aagtgatggc ttgtgcatgg aagacgaaca gttttccgtc aaacattacg
    26101 aagcacgagt gaaagacggc gtggtgcagc tgcgcggtta atgttttaac gggaggcgca
    26161 atgcctcccc tttttgcatg gtcctgtaat gaccttcggt atattgcagg acatttttta
    26221 aactttttgt tttatttttt tgtttttatt ttttaaagga taatcaaatg tttacagaca
    26281 ctattaataa gtgtgcggct aacgctgcgc gcattgcacg cctgtcggca aataacccgc
    26341 tcggcttttg ggtcagctcc gccatggcgg gcgcgtatgt gggtcttggg atcatcctga
    26401 ttttcacgct cggtaatttg ctcgatccgt ccatacgtcc tctggtgatg ggcgcgacct
    26461 ttggtatcgc cttaacgctg gtgattatcg ccggttctga actgttcacc ggacacacca
    26521 tgttcctcac ctttggggtg aaagcgggca ccatcagcca cgggcaaatg tgggcaatcc
    26581 tgccgcaaac ctggctgggt aatctggtcg gttccgtctt cgttgccatg ctctatagct
    26641 ggggcggcgg tagcctgctg ccggtagata ccagcatcgt tcactccgtc gcgctggcaa
    26701 aaaccactgc accggcaatg gtactgttct tcaaaggtgc attgtgtaac tggctggttt
    26761 gcctggctat ctggatggcg ctgcgcactg aaggggcggc gaaatttatc gctatctggt
    26821 ggtgtctgct ggcatttatc gcgtccggct acgagcactc catcgctaac atgacgctgt
    26881 tcgccctctc ctggttcggc aaccacagcg aagcctacac gctggcgggt attggtcata
    26941 acctgctgtg ggtgacgctg ggtaatactt tatcaggtgc cgtattcatg ggattgggtt
    27001 attggtatgc tacgccgaaa gcgaatcgtc cggttgcgga caaatttaat caaactgaaa
    27061 cagctgccgg ttaattacta aggggttttt acgtggatca tttgcctata ttttgccaat
    27121 tacgcgatcg cgactgtctg attgtcggcg gtggtgatgt cgcggaacgc aaagcaaggt
    27181 tactgttaga cgcaggcgct cgcttaacgg tgaatgcatt agcgtttatt ccacagttca
    27241 ccgcatgggc agatgcaggc atgttaaccc tcgtcgaagg gccatttgat gaaagccttc
    27301 tcgacacctg ctggctggcg attgctgcga cggatgatga cgcgcttaac cagcgcgtca
    27361 gtgaagccgc tgaagctcgt cgcatcttct gtaacgtggt cgatgcgccg aaagccgcca
    27421 gctttattat gccgtcaatt attgaccgct caccgctaat ggtcgcggtc tcctctggcg
    27481 gcacctctcc ggttctggcg cgcctgttgc gcgaaaaact cgaatcactg ctgccgctgc
    27541 atctgggcca ggtagcgaaa tacgccgggc aattacgcgg gcgagtgaaa caacagttcg
    27601 ccacgatggg tgagcgtcgc cgtttctggg agaaattgtt cgttaacgac cgcctggcgc
    27661 agtcgctggc aaacaacgat cagaaagcca ttactgaaac caccgaacag ttaatcaacg
    27721 aaccgctcga ccatcgcggt gaagtggtgc tggttggtgc aggtccgggc gatgccgggc
    27781 tgctgacact gaaaggtctg caacaaattc agcaggcaga tgtggtggtc tacgaccgtc
    27841 tggtttctga cgatattatg aatctggtac gccgcgatgc tgatcgcgtt ttcgtcggca
    27901 aacgcgcggg ataccactgc gtaccgcagg aagagataaa ccagatcctg ctacgggaag
    27961 cgcaaaaagg caaacgcgtg gtgcggctga aaggcggcga tccgtttatt tttggccgtg
    28021 gtggcgaaga gctggaaaca ctgtgcaacg cgggtattcc gttctcggtg gttccgggta
    28081 ttaccgcagc ttctggttgc tctgcctatt cgggtattcc gctcacgcat cgcgattatg
    28141 cccagagcgt acgcttaatt accggacact taaaaaccgg tggcgaactg gactgggaaa
    28201 acctggcggc agaaaaacag acgctggtgt tctatatggg gttgaatcag gccgcgacta
    28261 ttcagcaaaa gctgattgaa cacggtatgc ctggcgaaat gccggtggca attgtcgaaa
    28321 acggaacagc agtcacgcag cgcgtgattg acggtacgct cacacaactg ggcgaactgg
    28381 cgcagcaaat gaacagtcca tcgctaatta ttattggtca ggttgttggc ctgcgcgata
    28441 aattgaactg gttctccaac cattaattta accaaggcca gagaattctg gcctttttcg
    28501 tttacaactc tctttatata ttcagcagat aaaaacactc cccttaataa atattatctg
    28561 ctaatgggtt atattcaacg ttacagcgac ttacaaaatt aagtcattca ctcactgacg
    28621 ccgattaaat attcctg
//
LOCUS       DAZKJR010000058        28617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKJR010000058 DAZKJR010000000
VERSION     DAZKJR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.058"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..594
                     /locus_tag="ACHOWS_002516"
     CDS             <1..594
                     /locus_tag="ACHOWS_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFQ0048071.1"
                     /translation="EEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATESVSSGI
                     SSPAVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEGGGFLP
                     LKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNLDGQPE
                     GEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
     gene            complement(815..2299)
                     /locus_tag="ACHOWS_002517"
     CDS             complement(815..2299)
                     /locus_tag="ACHOWS_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0048072.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(2608..3012)
                     /locus_tag="ACHOWS_002518"
     CDS             complement(2608..3012)
                     /locus_tag="ACHOWS_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFQ0048073.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
     gene            complement(3180..3467)
                     /locus_tag="ACHOWS_002519"
     CDS             complement(3180..3467)
                     /locus_tag="ACHOWS_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0048074.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            complement(3464..3715)
                     /locus_tag="ACHOWS_002520"
     CDS             complement(3464..3715)
                     /locus_tag="ACHOWS_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0048075.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            4200..5066
                     /locus_tag="ACHOWS_002521"
     CDS             4200..5066
                     /locus_tag="ACHOWS_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0048076.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSDKMSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLS
                     DIVCWVDSLPYKIQAWRFYQFCPLGVGKEKRDKLEINTDIFKDKMVMLKKQHPNMPIS
                     WATFEERDKANVVMEPNFDIIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKN
                     NRFWLDKMEFTV"
     gene            5063..5770
                     /locus_tag="ACHOWS_002522"
     CDS             5063..5770
                     /locus_tag="ACHOWS_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014727861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0048077.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYDAYMVLWSRINELLTEPDIIAFINYPIAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            5772..6197
                     /gene="nudI"
                     /locus_tag="ACHOWS_002523"
     CDS             5772..6197
                     /gene="nudI"
                     /locus_tag="ACHOWS_002523"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFQ0048078.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(6561..7019)
                     /locus_tag="ACHOWS_002524"
     CDS             complement(6561..7019)
                     /locus_tag="ACHOWS_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048079.1"
                     /translation="MRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQRASRLAASV
                     KRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHR
                     SADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG"
     gene            complement(7160..8008)
                     /locus_tag="ACHOWS_002525"
     CDS             complement(7160..8008)
                     /locus_tag="ACHOWS_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0048080.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQLLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGGRPVIIFNARFDIRILKQTAAAHSDPAYWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLASAASQAGLTWEGLAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
     gene            complement(8054..8332)
                     /gene="yacB"
                     /locus_tag="ACHOWS_002526"
     CDS             complement(8054..8332)
                     /gene="yacB"
                     /locus_tag="ACHOWS_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFQ0048081.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(8332..8601)
                     /gene="yacA"
                     /locus_tag="ACHOWS_002527"
     CDS             complement(8332..8601)
                     /gene="yacA"
                     /locus_tag="ACHOWS_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFQ0048082.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDKMSRKVAGK"
     gene            complement(9508..10380)
                     /gene="repA"
                     /locus_tag="ACHOWS_002528"
     CDS             complement(9508..10380)
                     /gene="repA"
                     /locus_tag="ACHOWS_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFQ0048083.1"
                     /translation="MAALNQTSSTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAVYVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALF
                     AALDISEEAVASARRSRVEWENRQRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            11083..11724
                     /locus_tag="ACHOWS_002529"
     CDS             11083..11724
                     /locus_tag="ACHOWS_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFQ0048084.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            11865..12530
                     /locus_tag="ACHOWS_002530"
     CDS             11865..12530
                     /locus_tag="ACHOWS_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFQ0048085.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            12796..13416
                     /locus_tag="ACHOWS_002531"
     CDS             12796..13416
                     /locus_tag="ACHOWS_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFQ0048086.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAVFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            13443..15137
                     /locus_tag="ACHOWS_002532"
     CDS             13443..15137
                     /locus_tag="ACHOWS_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0048087.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIEGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            15218..15460
                     /locus_tag="ACHOWS_002533"
     CDS             15218..15460
                     /locus_tag="ACHOWS_002533"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFQ0048088.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            15577..15912
                     /locus_tag="ACHOWS_002534"
     CDS             15577..15912
                     /locus_tag="ACHOWS_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048089.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESFEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSITGKK"
     gene            16069..17139
                     /locus_tag="ACHOWS_002535"
     CDS             16069..17139
                     /locus_tag="ACHOWS_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFQ0048090.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNTLLTGQQLYGHMDWNRKILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTA
                     ADQAQVPPAVSASRAQSTTGTSGTSAPRTTTPAPVVTKPVAPPPQKVTPPRQWKMEKG
                     TTLKDGLARWASSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            17144..17581
                     /gene="pilM"
                     /locus_tag="ACHOWS_002536"
     CDS             17144..17581
                     /gene="pilM"
                     /locus_tag="ACHOWS_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFQ0048091.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSITLPSVIPDHVILWMN"
     gene            17612..19231
                     /locus_tag="ACHOWS_002537"
     CDS             17612..19231
                     /locus_tag="ACHOWS_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFQ0048092.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLQGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            19252..19455
                     /locus_tag="ACHOWS_002538"
     CDS             19252..19455
                     /locus_tag="ACHOWS_002538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048093.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLGFCAHFLQKSHS"
     gene            complement(19482..22520)
                     /locus_tag="ACHOWS_002539"
     CDS             complement(19482..22520)
                     /locus_tag="ACHOWS_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFQ0048094.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPEFTLKDVKRDVKYIIQTYYKNESTNLNLNIPK
                     STKYRLVKRMLSTLGFEQLTPSIKNELSERLNDVATICMEPKYIFDELLAFLGQRRIA
                     LPGYSTVQNLVSDALNFERKRTINILSKRIYPATAQKITSIIHDDGILNSIRGYNYST
                     RDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYYATIVKHSTTFRLKRHP
                     KWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFAKARATEEIDLINKKLK
                     FASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEFFDKNKIDVIEYQWQYI
                     DSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKLDAKDLRVIHPSE
                     KNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDDLIERSYWHKNKS
                     ILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDANEFVKKQPHSNRLQWSL
                     ASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRKNNLEADYNDLLA
                     CIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMANTVISDAIANLSVYKYW
                     TINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSNHVPLSTVVISPN
                     EYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFGYQFSPRYAKFKKIFNE
                     LFDVILESDKLIIKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRS
                     NKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQFR
                     GGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAWRH
                     ILLGGNYSFEDQVAITNLDSLLEGVDPLSDIGDIESEYDG"
     gene            22677..23258
                     /locus_tag="ACHOWS_002540"
     CDS             22677..23258
                     /locus_tag="ACHOWS_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0048095.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
     gene            23300..23629
                     /locus_tag="ACHOWS_002541"
     CDS             23300..23629
                     /locus_tag="ACHOWS_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048096.1"
                     /translation="MGANPLKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFG
                     RSGSQWLVVEVFGEKGRQHRSADSNPDMVEKMAARYRHAAGQHWSATLAEIERVKRIY
                     QARIKAS"
     gene            24233..24328
                     /gene="dqlB"
                     /locus_tag="ACHOWS_002542"
     CDS             24233..24328
                     /gene="dqlB"
                     /locus_tag="ACHOWS_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFQ0048097.1"
                     /translation="MINLLIIVLRAVVAVANALIAILELIRQFID"
     gene            complement(24695..24994)
                     /locus_tag="ACHOWS_002543"
                     /pseudo
     CDS             complement(24695..24994)
                     /locus_tag="ACHOWS_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            25300..25962
                     /locus_tag="ACHOWS_002544"
     CDS             25300..25962
                     /locus_tag="ACHOWS_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFQ0048098.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRIDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     IL"
     gene            26061..26345
                     /locus_tag="ACHOWS_002545"
     CDS             26061..26345
                     /locus_tag="ACHOWS_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFQ0048099.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFR
                     LPAYFEDILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
     gene            26375..26602
                     /locus_tag="ACHOWS_002546"
     CDS             26375..26602
                     /locus_tag="ACHOWS_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048100.1"
                     /translation="MRKRDDYYDGLIAGGTVAIAVATLLTLLITVFVNNKENSEFHEW
                     INSHCEKENVENTSFKCDDGDGNIYIVKKEA"
     gene            26977..27264
                     /locus_tag="ACHOWS_002547"
     CDS             26977..27264
                     /locus_tag="ACHOWS_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048101.1"
                     /translation="MATLQELIDLTPEQEKAWKRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDTGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDED"
     gene            complement(27375..27695)
                     /locus_tag="ACHOWS_002548"
     CDS             complement(27375..27695)
                     /locus_tag="ACHOWS_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048102.1"
                     /translation="MKAKTVIRTGVVVALIGIAGVLLEKIVWQTADTYPALLAGHRAV
                     TPLQSVDSFSASDARTICGLNDSDDIYDNADVAFKKNGTFVRCMNHPVSRVYKVIYTA
                     PRKS"
     gene            28194..>28617
                     /locus_tag="ACHOWS_002549"
     CDS             28194..>28617
                     /locus_tag="ACHOWS_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFQ0048103.1"
                     /translation="MTTRKSGFNWYVLEFCSARTARVFSHLERLGVTFWCPLTCSHYR
                     RSDKNCFRRKEVPLFPGYVFIKIDFNKTHSTTVTSVPWVKSFVSFGGEPVPVCEEIIL
                     GLRRRETNYWGEYSPIRGIPHELAEILLENNPMKRSFLF"
BASE COUNT         7725 a         6817 c         6458 g         7617 t
ORIGIN      
        1 gaagaaaaga aaaatcccgt cattgtggtg aaaaaacgtc gctctttttc accgccttcc
       61 ttagccgaaa aaaaagatgt tatcgcgcct gcactcactg aacaggcgac agaatcagtc
      121 tcctccggaa tcagttcacc tgccgtcgaa actcatatcc ccgaagcgcc agccaggaag
      181 aagaaaaaga aaagacaacg ctttccaaga ccatcccact ggaccagtga gttcactcac
      241 gattgtctgg agaaggtgaa agcgctgttt cctcaacttc gtgccgaagg gggcggtttt
      301 ctgcctctga aaacaggcat aacccgtgac attactacat atctggcagc acatccggaa
      361 acagcgctga ccctggacga atggaattgt gcagtatcct gcattaccag tcgccggatc
      421 tacctgcaga gaacctctgt tgcaggaact ccccgttata acctggatgg tcaaccagag
      481 ggggaagtat cggaatgtga tgctcaaaat tccaggcgct ggctggcggc gaaagagcag
      541 aggtggctga gtatgaaggc ccggcaggaa aatatttctg agaaatcaaa ataaccgcag
      601 gatattacag gaaacagact gtagagaaga gagcgttgta tattgtgcgt attatttaag
      661 cactcccgag agtaatggct tgattttgcg cgatcgcgac attttttacg tgtatccagt
      721 ttgacgtttc tggtgccgtg atcgcactga acgcattttg ctcccaatgc aaaatatgtc
      781 cggtatcagg agcaaatggc gggcaatttt taaactattt tatatctatt tcagcatttc
      841 ctggacagga tatatttccc attttcatag gggtgataag atgacgaaca acggccttat
      901 ctacacataa attgactccg gtaagagcta atgtatgccc atcaccattt attgtcagca
      961 acgggctgga aaatttctct gccatcttac gagcattaat ccatggtgtt gttggatcgt
     1021 atttgtgtgc tacaaacagt aaaccagagg gcagaactgt atttttcagc cgggttttat
     1081 tcaggttgct atgtattggc cataattcac aaaaatcagg tgaatcggaa cgtccattgt
     1141 caaagttaat agccgggaag gcattcgcaa gagcatcttt tcgggatttt cgctcttctg
     1201 gggttaattg ctcccccccc tgatcaacac aaaggattac ccccagagca tcacttgact
     1261 cttctgaggc tataggagta ctgagcgcag tttcaatttc attactgaca atcccctgag
     1321 agaactgacg tatggcagtt gcaagggttg gccatgatga acgccataac aaaaggtctg
     1381 ttgttaataa tatgagttca tctgaagata tattttctcc cttgctgtct aataaaggct
     1441 tgtgatgcaa ttttgataac agctcatgga actgagttat tgccttatct ctgactgaag
     1501 aaagtgggca actttttgta cgtgcacacc aggatgcaaa gcgatcgaac gtttcctgat
     1561 aactctgtgc ttgtttgagt tgccatgtga agttgtcccc caggtcatca atatcgacga
     1621 ccccatcaag aacgatagat ctgatgctgg atgggaaacg ttctgcatat aaggctgcaa
     1681 tttgtgttcc atacgaatat gccacagctg tcagttgctc gtcacccagg gcttgcctga
     1741 tacgatcaat atcatatacc gcctcgttag aaccaatatg gcgaataact tcagttccgg
     1801 tattatggat acagctgtta attttattta atacccgttg cttttcagtt atgttttcct
     1861 gaatccctgt atctgattgc tggcagttta ttgcaggaat ggactggccg actccccgtg
     1921 gatcaaagcc aataatatcc catgattcgc gaagatttgt taccggccag tcaaagttaa
     1981 tataaggatt aatgccaggt aatcctggtc caccacttat tataatgata ctccctttat
     2041 gcctgctttt tgctggcaat tttgtcagcg ccagttttac ctgctgtctt tttacagaag
     2101 aactatctcc gcctgtgtct gtatatttta atggaacaga caaataacca catagtaagt
     2161 caggagacgg tttttcctca ccaaaccagt tactgaatgg agtggtcata caggattgcc
     2221 actctatcga ctgggcagat actgttgcgg gcagaagtaa ggttaaaact atcttgaaat
     2281 gagcaattat ttttcgcatt gtgtctctta atatcggtat aaagataaag tttgaatatc
     2341 ttaaatccgg tgttacggca ggaagttaca cgttacggat ataattcaaa tgattacaaa
     2401 gtcagggtat attgtttaac tccccggaac tatgtaagcg tcaccgtctt gtgacgtctt
     2461 atgatggtta ttctgtctgg taacaatcgg ttattttcct ggtatcccac tgacagcaaa
     2521 agaacaagac tgctgaacta taaacggcca tgatgaatca tcacgattcg gccccagact
     2581 tgtccgtcag gtaatttttc ggttcaatca ccggaagaaa ttatcgacgt aatcatccca
     2641 tgaatgcccc tgaacagacc attcatgatt aaccggtggc gggaatccac ttcgacagta
     2701 gccgttcttc ccctcaaaat cgaatacccc atgtggatat ccgtttatca gcagaacaca
     2761 cttcaggcaa tcctcagacc agccaatctt cacatcagag gggatgtgag catctgaaat
     2821 gtcttcaacg ttatatatat gcacagcatc cctgacaggg ttaccttcaa cggactcatc
     2881 aagtgcatag aaatatccgg tattcccgtc gtcttcaaat acagcggcca acattccatc
     2941 aggagctaaa ctttttatcg ccatagcctc accaactatg agttgagcct gtgccgtcac
     3001 aagtattgtc atcaaacatg tccctccgtt tcaatttgtc tggtaatata aataacccca
     3061 gactcatccc ggcaggtaaa atcattctct caacaaaact tttctttttc cgcctcaggc
     3121 gaggccacag agagaataac aattctgaga gttatctgcg ggaaaggcta tttatgttac
     3181 tatctcattc tctcactggc gagataataa acgttactac gttctcgttt acctaccgca
     3241 acaaccgcaa taattaacat atcatcaatc acctgataaa ccagacgaaa tcccgatgca
     3301 cgaagtttaa ttttgtagca gtcctttagc ccacgtagtt ttgcagacag tatatgagga
     3361 tttttgctac atttttttag ttttttcaca aactgttgtt gaatggtctt atccagtttc
     3421 agccattctt taagcgcatc atccctgaat tttatcgtat aagtcatgaa taactgtcca
     3481 ggtcaacatc atgcagaggt tgactgctac gttcagtaac cagtttcacc agctcctggt
     3541 catccagagc atcaagcatc ctttcataca gttcagcagg aacacagtag aatgccggct
     3601 gattgcgatt cagaatggcg accggataac catcaccagc accgactgtc gccatcggat
     3661 tttttttcag ttcactgaca cttgcactga tgtcagacag aatgatgtta ggcataatgc
     3721 accttttaag actgtttaat aagtcttaaa gatacatctg aaaggacccg ttaacaagtc
     3781 tgtattactc atacatttgc aaataaattt aatgctgatt ttttactctg atgccgcacc
     3841 agcgcatgga aggtggcaca caatggttac atataacaca ggagtaaaat cggcgtaagc
     3901 cggtagaaga actaaatatt ctccagttat ctcccgtctt gtgaggcata cggcctgtca
     3961 gtgatgaaaa tgcttctgtg cacaattttc actggtgtga ctgatagcgt ccctccgatc
     4021 atgggcttcc agtaatgcga ttaccggaac tcatcattca tgagttcctt gcgcaaccca
     4081 ctgctctgat tactggcaga tattatatcc ctggtaagcc gaaggttgcg ctttgtcatg
     4141 gtctgcaata accattatgt tttaatggtt attgatgtat catagtcaga ggacttgtta
     4201 tggatattag cacccttctt ttagatttca gaataacttc acattgtaac ctaggatgtg
     4261 atctgtgttt tcgtacacct ggaattcagg actcttctct tgctagtgct cgtaatgtaa
     4321 ttgagcggat gtataaaatg ggattccgtc gtatcggttt taccggaggg gaacctacat
     4381 cccggagtga tttcattgat ctgattgaat acgctaagca actgggtttt atgacatatc
     4441 tctccacggt cgggcatcga tttattatgg atcatgctcg gttaaatccg gtgttagact
     4501 gggtaggaat accaattgat ggaattgaca gtcttgtcaa ttcagaaatt cgcagtgata
     4561 aaatgagtaa ccaacacagg gttatcaaaa atatattaac atggttaccc caaaataata
     4621 atcgaattaa tataaaactc acgaccgtcg tatctcagtc taacattaat catctcagtg
     4681 atattgtttg ctgggttgat tctctgccct ataagatcca agcttggcga ttttaccagt
     4741 tctgtcctct aggagtcgga aaagaaaaaa gagataaact ggaaatcaat acggatatat
     4801 ttaaagataa aatggtaatg ctaaaaaaac aacaccctaa catgcccatt agttgggcaa
     4861 ctttcgaaga acgggacaaa gccaatgttg ttatggaacc caattttgac attattatcc
     4921 ctgatggaga aaactatact ctgttgggaa atatgctgac agattcacaa gatcatataa
     4981 tcagtgcaat tttcggaaat aatgaaatca tgcaaaaatg taagaataac cgcttttggt
     5041 tggacaaaat ggagtttaca gtatgagata taattacttg tatatggatc cttggtcagc
     5101 agataaaaaa gagaagaata aaactcgaaa tcctctgtat atatgtctgt cgggtaattc
     5161 cggtgtaggt aaaagtactc ttctgagacg catgtcaaag gagttattta aggtagatcc
     5221 agacactgtc gcgatagatg aaaaaagtgt ccaccacact ctccttcctt acctttttga
     5281 cgataccagt aactacggtt atctaatcca gcttaatttt atgatacaac gagcattact
     5341 gataaaatct tggctggata aagggtttaa ccttattatg gagagatccc atattgagga
     5401 ttatattttt atcaacttca tgctgaagtc cgaatatata aataaagcac aatatgatgc
     5461 atacatggtg ctctggagta gaataaatga attacttaca gagccagaca ttatagcatt
     5521 cattaattat cccatcgcac attcactcat gcatctcaat gatgatgaaa ttcatggtgt
     5581 acggcctgaa gaatttccag atgaggaaat gaagataaaa tggatcacag gatggcatgc
     5641 ggaatatcaa cattttactg ataatcttcc gttacacctt cgtgagcgga ttatggtatg
     5701 tgctcacccg gatgacattg atgatttttc tgataaggtt atccaaaaag ccatgatgtt
     5761 gaggacataa aatgcgccag agagtaatag tgtgccctgt gatacaaaat gaaggtgagt
     5821 ttctactttg taaaatggcc tctgatcgcg gagtattccc gggccaatgg gcactgcccg
     5881 gtggaggaat ggaacccgga gagactatgg aaacagcatt gaggcgtgaa ataaaggaag
     5941 aactgggaaa agaactgcat atcactgacg taaaaccctg ggcctttcgt gatgatatcc
     6001 gtacaaaaac gtacccggat ggtacccacg aggacattta catgatttac ctaatattcg
     6061 actgtatcag tacaaaccgt acagttacat ttaatgagga gtttcaggat atagtctggg
     6121 catctcctga caccctaaaa tatatggatt taaatgaagc aacccggatc acattttctc
     6181 agaaaggtat cctttaaact ccaatgctaa tcgtgcaact gataacggct tccacaacag
     6241 ggagccgttt tcttatgcca ctactcaagg atggggtttg agggccaacc gtacgaaaac
     6301 gtacaattct gtacatctac gcaaggaaat gtacggaagt aagccaaatg gttatcggac
     6361 ggtcggaaca tttcgtacag tctttacgtt cagtgagtta gctggcctgg atgtaaccac
     6421 tcgttcaact caattctctt agcgtgggaa aatttccgaa ttccaagcgg accccttatt
     6481 gtacgttcaa cgcaccatca ttaatgctaa tgtatgcata cattagcatt aatgatgaag
     6541 gagaagaata acaatggcag tcaacccact tcttttttgg atttcttaat tttcgtctga
     6601 taaagccgct ttacccgttc aatttctgcc agcgttgcgg accagtgtag ttcggcggca
     6661 tgacgatatc tggcagccat tttttccacc atctccggat tgctgtccgc gctgcggtgc
     6721 tgacgcccct tctccccaaa aacttcaaca accagccact ggcttccggt tctgccgaac
     6781 aatgccttgc acaaacgtct cacagtcagc ggggttaaat ctggatcggg atcgtaggca
     6841 atggtagcaa aaataaagcg cagcatttct gatgtcttgt aacgctttac gctggcagcc
     6901 aggcgtgagg cacgttgcgg tgcttcccag cggtcgttac tgtaattagc cacttcagcc
     6961 agataatcct tttcaagttt ttcagccgta tagcacctgc cgcctaaaaa aagccgcatg
     7021 gcctccctgg ttttgtctct cacatatcat ccctcattaa tgctaatgta cgcatacatt
     7081 agcattaaca tgctgacagc aacaagcggc agatttacgt ccggataacg taaatgattg
     7141 cggtatacag gccaggcggt cagatttcca gccgtgcctg ttcctgcagc agttccagat
     7201 gatatgcagc aatagcgttt accacccctg ccgtcatccg tgcgtcggcg atcgctgagt
     7261 gtgccagccc ttcccaggtc agtccggcct ggctggcagc actggccagt gaaatcgtgc
     7321 catagcggtt ggtggcccca taatatcctg ctgccagctc catcgcacaa tataccgtca
     7381 tttcttccag ccagtaagcc ggatcgctat gtgcggcagc agtctgtttc agaatgcgga
     7441 tgtcgaaccg ggcattaaag ataattactg gtcggccccc gatagcatga cgcagttgcc
     7501 gcgccacatc tgtccatgat ggtgcgccgc agagagcctg ctcactgata ccatgcacag
     7561 cggcggcctg cgccccgatg gcgaccgtgg gcttcagtct ggtttcaaat accacctgac
     7621 cggccgcatc agtaaggccg atttccaggg cttccgcggt attatccagt cctgtcgttt
     7681 cagtatccag aaagagcggt gccagactca gccagtcata tgcctgccgt gccatccggc
     7741 cactggtgct gttaagtctg gataaaacgg aaagtcgctg gccagccaga agttgtttac
     7801 tcgtcagtgg gcgtttctca cgcatcggaa cacaatctgc aattcggtat acagcgaacc
     7861 tgccaccata agtgttttta taccacttaa ccggctcagc accgggggca ggcttcattc
     7921 tgaattcatc gcgcagacgt cctttcgaga aacatctgtc ttcagcaatc gcctgcgcac
     7981 gtctggtggc cagctcatca ttattcatgg ttcttgcatc tccgtcagac gtttttatat
     8041 ccggacagcc tctctatggc cacttctgtg cagtatgcaa tacacgcagg atatacactt
     8101 ttccccactg gctgtcgacc tcataaacca gaacaaaatg cgggtgaata accagttccc
     8161 gggtaccttc cacacgaccg ttgcggcctt tatacggctg cccggccagg ctgctggctg
     8221 aataaccaat ccgttcgtcc agttcaatgg cggccatcag gttctgctcc gcaatgtact
     8281 cacgaatgcg cttcctgtcc tggctggcca gcatagtcca gaatatctcc atcatttacc
     8341 ggcgaccttc ctgctcattt tatcccgcca tgctgcagca gaggattcga ccatgtcatg
     8401 gggaagcaca tcgccgtgct cgagttgctg acgtcctgcc gcaacctggt cgcggaacca
     8461 ggtattatgc tgccgttcaa cggtctggcg cataaaatcc cggattaact gagagccatt
     8521 acggtccatg ctttttgcag cggccataaa agcatccttc agttcagcgt cgattcttaa
     8581 actcatatta acctgtgcca tttatcacct cgtcattact acgtatatac acagtatata
     8641 acgattcaga tatcgtcacc aggatatgcc gcgccagcgg catggaaggc ggcactccat
     8701 tgtttcatat gataccggag taaaaccgcc gaagcccggc gtaagccggt actgattgat
     8761 agatttcgcc tatcccattc ccaccctgct aagccatatc cgctttcagc catgagagag
     8821 cttctgtgcg cggtcggagt ggtcccgacg agggtttacc cgaagtcggg gcgtatctcc
     8881 gcgttagcgg gccgtcaggg ccgcttacga gcgtgtattc agtacgtcca gccagaagac
     8941 tgacagcgat ggtgttgttt agttacaaca ttcataatta aaagcgatgt tgttcccgcc
     9001 ctttgggcgg gggcggcctc gcttttcagt tatgaggggt gggcacggtg ctgcgtttgt
     9061 gtagtgtttt tattcctgat gtttttttgc tgtggtttca gaacggtgag gttcggggcc
     9121 cgcgcgttag agtgggaggt acatatttgc ctgtccacag gacaggcatt gaataggttt
     9181 tctttttaaa tggaactaat taagtagttt aaaggaaata cagtactggg gtttaaaaga
     9241 aacattgcac cctgtaacgc ctgcggctgg cgctttatgg aatgagcggt tgtaacctta
     9301 cggaaaagtc ccttgcagtt aaatgtggat aagcagaatc ccccgtctgt gagacgagtt
     9361 ttgcgtgaaa aacaggggga gcaaggcgag ccggaaggca gatgatgagg ctgttttttg
     9421 tgtgctgcgc tgcttttttg tgcgtcgccg gccttcagac gtgcggaagc ctccggcgcg
     9481 ggccggatta ttcagatgag gtaaaactca gggtgaggct gtggccagcc gtgtgtagtt
     9541 gttgttccgc gacattatca tgcgttcctt cacgcgacgg tcgatttcgc gcttcacagc
     9601 ctccaggctt ccccggaaac gcccttccgc gatttcacgc gtcagctgcc gtttcaccag
     9661 ggtgacaatg tcctgccggg ttctgcctgc atcacggcgc gcacgggcac gtttcatgcc
     9721 gtgggactta agctctgcct gataactacg gaaacgctcg cgcacaaaac gccaggccct
     9781 cgctatcagt tcatccatac ccagcgcatc cagcccctgc tttttgcgct gcctgttttc
     9841 ccattccacg cggctgcggc gtgcggaggc caccgcctct tcggaaatat cgagggcggc
     9901 aaacagtgcc ggtgtgaacg tgatgtcggt cggaatataa cagccaataa gcgggtcata
     9961 ttccgtctgg taggtaatta gccccagctc tgacaggaac gtcagggccc gggtggcacg
    10021 ggtgatggag agttttccgg cagcagactc cgtcgccagt ccgcactcaa tggccagcgt
    10081 ggtgatggaa cactggacgc ggttggccag tgggtcatag tggaaacaca gcccctgcag
    10141 cagcgcatca atggcacgtc ggcgcagtac cggtggcatg cgccgacgca gcccccggga
    10201 acgggcatac gccacataca cggcgaaatc gaaacgggaa gtgaaaccca ccgccttttc
    10261 catcagtttt tcgcagaact tcagcgtccc ggcatcttca cggggtgtga agaccggatt
    10321 cgggttcttt acctgacggt atgtgctttc agtagatgag gtctggttca gggccgccac
    10381 aagctgtcgc ttatggcgga aacagaatgc cgtgcgttaa tgcactgcga agggttgaat
    10441 ctctggtacg gtttcatata tacttatccc gtattctgtg aggcccccat aatcttctct
    10501 acgttccgcc aagttcgata agaagattat gggggttttc ctttatggtc ttctgaaact
    10561 tttttcagcc ttccggcacc ggcaaactgc cgtcgtttct gccatacatc ctgaatgcct
    10621 tatggcgcat cctgccgttt tctgacttcc cggatcctga cgatcccaaa agccactgca
    10681 taacgatccg caatataccc cttttccacc gctttgaaaa gccgacacga tttctctgat
    10741 ttaccaggcc tctcattaac cacctaaaaa acgtcgtttt tttgaactat gtcaaaaaaa
    10801 tagacaattc cacaaacgat cttttatgct gtgtttttca tcatttttag tagaagagat
    10861 cgcctaaaat ttttaaacga tctaatcctc cagacaccgc acacaaatca gcataatatg
    10921 ctgatttatt aaatataacg atctatttaa ctgcttatct tcactcttgc cacagataca
    10981 tccttatgta atatatttgc cagccattaa tcctgtttgc agaagttgtc ggttttcgcc
    11041 cgcctcgaaa ccgtccacaa ggcttacaag agtgcgtaaa tcatgtgcct gtcagaactg
    11101 caggccgggc tgaacgaggg tcagccgctg tttccgacac atgctttatg tattcgccgt
    11161 tcacaacaac atcgaagccc ctctgcgggg atcgattttc gcggagcgtt ccggattttg
    11221 aatatcagtg aattacatca acgcaactgg tatctggctc agtacatccc tgccggcaaa
    11281 aaccgtgaac atctcttttc ctggctttcc gaacaacacg tccttccctg gacacccctg
    11341 atactgaaaa aagtccgccg cacggataaa gtctgcggtt accgtcgaca tatacacgcc
    11401 gtgtttcccg gttacttttt cctgaaagca gaccccgaac gccatagctt cactcacctt
    11461 cgccgtcaca gtgcctttct gaacttcgtg aaaatggccg gtgaaatcaa aaccgtcagg
    11521 gaggatatcg ttcagtcttt gatgaaagtg tacccggatc ctgcgctcaa tccggcagcc
    11581 agggaggagc tggatgctgc ctccaccctc tggctgacaa aagcgcgtta ccagtacctc
    11641 ctccggcttg atgcacagcc tctgccggag tcgaggatcg ccctgctcct gcacctggta
    11701 tctgatgacg gagcgttgac atgaaaattg ctgtcttaat ttgacgactt ttcccggcct
    11761 ttatggccgg gatttttttc ccttctgttt ccacaatgcc cggcaccggt gacacctgag
    11821 ggtgtacgga gagcggctgt cgggcatttt ttcaggacgc ctttatgacc attctatccc
    11881 aggaaaaccc tgtcattctg ttactggaga atatgctcga agcactctcc accgccccgg
    11941 acaacataaa caacgaacgg cgtcgccggc gttacctgct gaactggctt gacactgccc
    12001 gtcagatgcg cgagttcaga ggaatggctg aagagttcac taccctgcga aaactgctgg
    12061 ccaccaattc agacataccg gtagcaaaca ccctgaaatc actgctggaa agtggcaata
    12121 acgcagcatt atacgacctg taccgcttcc gctcggctct ttccgcgtgc agaagtctgg
    12181 gatggcagat tggcacgtgc gcatggaacg accagctgct gagtgagaca ctttcccgtg
    12241 cgcaaacagg taaacgtcat atcctgcagc tgaacagtac cagagacacc ttttccacca
    12301 cgggcagaat gctcagaccg gtggcatttt cattaattca tccggcatcc ctcgaatctt
    12361 cagaagtgga agagatattc cgggatgaag gttttatact cgcgcacgga cgcgaatgca
    12421 gcctgaacgg cagcggcaga aacatgctgt cacgcattct tcatgtgggg cattcaggtt
    12481 taccgaaagc cgaatggggc atagatcaca gcaatcacct gttacactga aataccggga
    12541 cagccacaac ggcagccact ctccgctgac tttttttcgc aaatcgcggt ttgctgatgt
    12601 ggatgtccca ttttttatta tccgtcctct cctgttgtca taaagcacat tccggcgtga
    12661 ggcttctgtt gttatcatcc cctcctgcag acatcccctt gtcttctgat gactgacgat
    12721 atgcttcttc tgacaattaa agaagcggaa gcttcacgca taatgttgtt tgactgacct
    12781 gccacaggag gaaaaatgac actgacgtta cttaccgcgt ataacgcacc ctatctggca
    12841 gccctgactt ttgttctgct tactggtatc gctgaactta tcgccctgct ctgcggacac
    12901 tccctctctt cagctatgga tacacctgac ttaccggagg gtctgacagg agaggcgctg
    12961 gactggctga acatcggacg gatccccctg ctgatcgtcc tctgtatgct ggcagttttt
    13021 tttggtatat ccggcattct cctgcagggt cttattattc atctgctgca ggctcctgcc
    13081 ccgaacattc tccttgcacc actttgcctg ctgctgacat gtcctctcgt acaccgcacc
    13141 ggcagactga ttgccccctg gttaccccgt gatgaaacaa cggcgctcac cgaggaggca
    13201 ttcatcggct gtatggccac catcaccggt tatcacgcag tggcgggttc cccctgtgaa
    13261 ggtcgcttca cagaccgctt cgggcaggtt cattacctgt tgctggagcc ggaggccggg
    13321 aaagaattca gacgaggaga caaagtgatt attgtctgcc gtctttccgg ttcccgctgg
    13381 ctgggtgaac tgaatccctg gccggatgtt ctttaatccc gacatataca gaggttctca
    13441 ctatgtttaa tcttccggga tggttgttta ccgttattgc gctggtcctc accctgttcg
    13501 ttatcgggct gatatttgcc cgcctgtacc gtcgggcatc ggcagagcag gcctttgtcc
    13561 gcacaggtct gggaggccaa aaagtggtaa tgagtggcgg tgcaattgtt atgcccattt
    13621 ttcacgaaac aattcccgtc aacatgaata ccctgaagct ggaagtcagt cgtgcagcgg
    13681 ctgaaagtct cattacccgc gaccgcatgc gtgttgatgt tgccgttgcc ttttttcttc
    13741 gggtaaaacc atcagccgaa ggaatatcca ctgcagccca gacgctggga caacgcacgc
    13801 tcacacctga agatctgcgt agtctggtcg aggataaatt tgttgatgcc ctgcgggcca
    13861 cggcggccag aatgagcatg caggatttac aggatgcccg cgagaacttt gttcagggtg
    13921 tgcagaacac cgttgcggaa gacttatcca aaaacggtct ggagcttgaa agtgtgtcgc
    13981 tgacaagttt caaccagact gcacgggaac attttaatcc tgacaatgcc tttgatgctg
    14041 aaggcctgac ccttctgacc caggaaacag aacgtcgtcg ccgcgaacgt aacgaagttg
    14101 agcaggatgt tgaagtggcc atcagggaaa aaaaccggga tgcactctcc cgacgtctgg
    14161 agattgaaca gcaggaagcc ttcatgactc tggagcaaca acaacgggta aaaacccgta
    14221 cggcagagca gagtgccagt attgctgcta ttgaagctga acgacgtcgt gaagcagaat
    14281 ccgcgcgaat tctggctgaa cgtaagatag aggaagccga aatcgaacga cagcagatag
    14341 tgcgtacccg ccaggtcgaa gccgaacggg aagtggccat acgggaaatt gagcaacagc
    14401 aggcaaccga aatcgccagc caggccagag ccattgccgt ggcggcaaaa tcggaagagc
    14461 agtcacaggc agaagcccgg gcaagtaaag cactggctga agccgtacag gcccagcagg
    14521 atgtggaaac cacccgccag actgcggagg ccgaccgtac caaacaggtt gccctgattg
    14581 ctgctgctca ggaagctgaa acgaaagcgg ttgaactgac ggttcgcgcc caggctgaaa
    14641 aagaagcagc cgaactccag gcagcagcaa ttattgaact ggcggaggcc acccgtcaga
    14701 aaggtctggc agaagcggaa gcacagcgcg cactgaacga tgccattaac gtgctttcag
    14761 atgagcagac cagcctccgg ttccgtcttg ccctgctgga tgcattacct gccgtcatag
    14821 agaagtccac agaaccaatg aaatccattg aaggcattaa aatcattcag gtggatggcc
    14881 tgaatggcgg tgttacccgt ggtaaaacag caacaaccga cataagtggc ggaaatctcg
    14941 ctgaacaggc ggtaaccgcc gctctggctt accgtacaca ggctccgctc atcgactccc
    15001 tgctgaagga ggtggggtta tccggtgaag gacttacagc cctgacacgt ccactggtca
    15061 tgccagcccc ggaaaaggtt ccggatgtca gcgaaccggt aaccgctccg gataactcag
    15121 aaccacaaca ggtttaatcc ccgtgccgga atttttccgg catctttttc acaaagaatg
    15181 ccctcacagg atattctggt ttaaaagagg attgaaaatg tccgcagaaa aattcagagt
    15241 tgtcatatac accaatacat gtgaaaagaa agtcagttat attcctgcaa cagaagatat
    15301 aaacatcgct ataatgccat ttcagacggc agacaatggc ttaattattg tgtgtcagaa
    15361 ctcccgtata atacgacgca aagagcgtat acccggaagc agctgtgaat ggaaagatgt
    15421 gacattagca ccaaaatcca gaaaccgcgt tcagttatag cctgacagcc tgctttttat
    15481 cccccataaa agggggataa aaattaaata tttcacaacc gcgatatttt tcaggaatgc
    15541 agattatttt atgttatgac caacagagaa acaggcatga gaacaatcac aacgcgtgaa
    15601 caattattaa ttaatgggag agtccacgaa agaatcgcca cacatatagt tacaggagct
    15661 catgggtatg aaacgctttg cacaaatggc tataacgtca gatacaacaa ggcgcagcaa
    15721 ctggttgaaa gcttcgaaaa aatagctgaa ggcaaactac ctgttacctg tcctgtctgt
    15781 ttcgccatat ggcaggatgt acacaggttc acccacgttg actttgacac acaatcgggg
    15841 aaaagtgatt ttattgatac ctgccattcg gagatcataa ccggaaatgt ttcaataaca
    15901 ggcaagaaat aaaataatgc tcccgttcta aaatacaatt cactcttata atccccccac
    15961 gaacatgaac gttcattatc aataacccga tgaaatgata ttcatctgaa ggccagttac
    16021 ccgccacctg tcttataccg taacccgtaa tataccggat tttattctat gcaaaaaaaa
    16081 tcacttacac tccttctgcc gtcattactc agcggctgta cggcatttaa tgccacgcct
    16141 gttcctgaca ccacacctcc ggctctggtg ttcgtcgacg gacaaattcg cgaaagcgct
    16201 gccattatcg cccatacgca aaaacgtctg cttccgccac cggtgacccg ccctgttacg
    16261 ccgccccgcc cgacaacaac agcgacgccc acaaccgcag tcagcagcca gagtaccgca
    16321 ctgcctcccc gcccgctcct tcaggccaca cagctgcccg cactggttat gaccggtaaa
    16381 cccggtcagt catccgtcgt ttatttacgt gaggcacgtc agcgctccgt cagtgactgg
    16441 atacgccagt tgctgccacc cggctggcag ttccggcaac agaacgctgc cataccggct
    16501 ctgaacaaac gcctccccgt ctggtccgtt aatgaccagt ggcaccgggc actgaatact
    16561 ctgctgaccg ggcagcaact gtacggacat atggactgga acaggaaaat actgactgtc
    16621 acgaccactg cagcaccgcc tgttgacctc acggcaccgc agggttcaca gaaaacagcg
    16681 gacacaccac gtaatccgtt caggagcaca accggaacgg cagcagacca ggcacaggta
    16741 ccacctgccg tcagcgcttc ccgggctcag agcacaacag ggacatccgg tacatctgcg
    16801 ccccgcacaa ccacgccagc cccggtggtc acgaaaccgg tagcgccacc accacagaag
    16861 gtcacaccgc cacggcagtg gaaaatggaa aaagggacca cgctgaagga cggactggcc
    16921 cgctgggcgt cgtccgaaaa atgcccccac ggcacctgga cgctacgctg ggaaacgccc
    16981 gttaactaca gtatcgatgc ccctctggtt ttcaccggca gtttccgcca ggcgctggaa
    17041 cagctcttcg cactctacac cccggtggaa aaaccgctgt atgcccggac aagcacagca
    17101 caatgtctgc tgcgggtgac agatcgtcca caggaataac cttatgggct ggttcatcgt
    17161 gttcatcatg gtcatcacga ccacaggcaa tttctggttc accagtcagc tctcccgcag
    17221 cgaacaccgt catcaggccg cagaaaacac acaacaggca gcaacgttca ttcgttatat
    17281 gaacgccatc aatgattacc tgcatcagca tccggaacga cggacagccg gagggcggct
    17341 gacatccgct cagctcggga ttcccgggac ggataccgtc agtcatatca tcagccagca
    17401 acgtgttttt gtctgggcca cagaaacccc gggcctgatg gccgcgctca gggaacagag
    17461 taatgactct gctctgctcg gcagggtgga aaacggccga ctgctggaca ctgccggcag
    17521 agcgctttcc atcacacttc cgtcagtgat ccccgatcac gtcatcctct ggatgaatta
    17581 gtcacgaatc tgaataaatc acggatctcc catgaataaa aaaattcttg ccattatgat
    17641 tgccgccgct ctgttacagg gctgtgccag ttacagacag cttgagagca caagaaagga
    17701 agccgttgcg gaacaacaac gggcacggga aatgatgtct gatctgcgaa cccggacgcc
    17761 ggttgttaag gaatcctcac gacaatggat caatccccgt ccggtcatgg ataaaaacac
    17821 cggcaaaacc gttcccggtt gtcccattgt cattcatacc cgtcagagca tcacccttca
    17881 gcaggtggtt cagcggatca cggaaagctg ccatattccg gtacggatca cacctgatgt
    17941 actgacatac ctgaatacat ccacaaccgg cagcacacaa caaatccagg gcgcgttacc
    18001 cccaccggat gcgagcggca tggttcccct tgccgccatt ggcgcatctg acacgccacc
    18061 atcccgtctg cagggaaata ccgcagcact gtctgaactg aatgcctccg gcctgacgac
    18121 cctgctgaat gcggtctcct cccgtctcgg tatatcctgg cgctatgacg gctcgcatat
    18181 catcttctat tatctcgaga cacagacctt ccccatcacc tatatggaca gtcaggtggc
    18241 ctataacgcc actgtggtca gcggcaccat gtcctcaaac ggctcatcag gaggcagtgc
    18301 atcagatgcc atttccggtg atgccagcaa tacccagacc accaccgtgg atatgaagtc
    18361 tgcgctgtat caggatgtga aaaatgcggt cacatcaatg ctgaccccgg gtatcggacg
    18421 catgttcatg tccacgggct ttatcaccgt cacagataat ccacaggttc tggaaacggt
    18481 ccgggcattc attgaaaaac gcaatgaaga gatgaaacgt caggtggtgc tgaatgtgga
    18541 aattctgagt atccggaaaa cccgcaatga gcaggccggt atcgactgga acgcagtctt
    18601 cagcgacaga acgctgggac tgagtctcgg cagcacattc accagtgcag ccagtgatgc
    18661 catcacaggt ggtgtttcca ttgtggacgg taaactcacc ggttcaaaag ccttcctgaa
    18721 agcgttgtct tcccaggggg atgtcagtgt ggtgactcag aactctgccg tcaccaaaaa
    18781 cctgacgccg gtgccaatgc agatcgccaa tcagcagagt tacatcgaga gtgtgacgac
    18841 ggataccact gccaatgtcg gctcatccac ctccctgaat gcggccacca tcaccaccgg
    18901 ttttaacatg acccttctgc cgttcattct cccggactca cagacactgc agttgctcta
    18961 ttccatgagt ctctccgaca aaccggttat tgagaattat gagagcggtg gaagtaaagc
    19021 ccagctgccg aatgtggatc tgaaaaccat caatcagacc gtggatctga aatccgggca
    19081 gacggtcatt atcagtggct tccagcagtc aggacgtcgc agcggaaaac agggtgtagg
    19141 cacgccggga ttcttcggtc ttggcggtgg gatcaacagt gaaaacgacg acaccattct
    19201 cgtggtgctg atcacgccca atatcatctg atttgcacaa ggaggacgat cgtgacggat
    19261 atttcaccga cagggcggca ggtgtttccg ctgacggcag ggcgcaatgt gtttctggcc
    19321 ggtctggaat ggaaaacact gccctcacag taccggcatg cccgcgactt tgcccgggca
    19381 cagaaagccg accttttcct ggcctgtcag tatctggggt tctgcgccca tttcctccaa
    19441 aagtctcact cttagcataa aaaccattat ttatcatgtt gttaaccgtc atattcggac
    19501 tcaatatctc cgatatcact tagtgggtct actccctcca aaagactgtc caaatttgtg
    19561 atcgcaactt gatcttcaaa agaataattc cctccaagga gaatgtgtct ccatgctact
    19621 ggggataagt tagccagaac atctatcgct tttttattat tttctttttc aaatttctcg
    19681 accagcctgg ataatattgc tgaattataa tagattacac aattagctat taacctcgca
    19741 cagtcgttcc attgatctat ttggtagtca ttaccaccgc gaaattgatt tccatttact
    19801 gatgctattg ctcttcttag ttgatgataa gcttcacctc tgttcagtgc ttgttgcaca
    19861 aattgcctta aggttttatt gtcagcataa tccaggacat atatgcattt tataactcgg
    19921 tcatactccc gaagtgctgc taatgtcttg ttgctacgct ttgtattaga taacttctta
    19981 acaaccgtac tttgaatggt tgtttttctg cttaaagaac aaataatatg ttggatgttt
    20041 tcccattcat gttcaatgag cttaaagtta aattgttttt taagttttat aatgagttta
    20101 tcactctcca gtataacatc aaataattca ttaaatattt tcttgaattt tgcataacgt
    20161 ggtgaaaact gatagccaaa aatgtcaaga atcgcaaaat taacgttgtt tataccatga
    20221 ttgtcagtcg ctaatgactt tggttgtatt tcactggtgt tgttatataa caagtcaaat
    20281 gcaaaatgac cttcatattc atttggagaa attaccacag ttgataatgg cacatgatta
    20341 gataccagtg tcatcgcaga aacccctttc ccctttctga aatacttggc agaataacgg
    20401 gctttaaatg tatttattct gcaagtgtgc ttctgcccat caacacttcc aaatggagaa
    20461 tcttcattta tcgtccagta tttatataca gaaagatttg caattgcatc agatattact
    20521 gtgttggcca ttcctgttgt ttctggacga atgaatttgt cattcacatc acgtagtatt
    20581 ccaatactac ggtctgatgc agatgccatt ctgtgaacac cataatttgc gccattacca
    20641 aatatacagg caagaaggtc attatagtca gcttcaagat tatttttcct ggatgatatg
    20701 gcattaaaca cagacaaaaa attcgtctca ttgtttacat acttcattac gtcaactatt
    20761 ccgatatttt ttatctgatt gtaaatcgga ttatcgatgc tggtcttcca ttttttactg
    20821 gccagactcc actgtaatct gttggagtgt ggttgttttt tcacaaactc attagcatct
    20881 gagtttattc caatggttat acgagccagt ttatcgctaa aggtattctc cagctcatta
    20941 agagtctgcg ttattggtgt gataagtcgt tcaaggcctg ttttctctat caaaatcgat
    21001 ttattcttat gccagtaact tcgttctatg agatcatctt ccagtctctt gttttgttca
    21061 ctctccgcag cataaaagac accgttttcc agatggttag ataccctgct gtacatatga
    21121 aattcgaatc gttgagtgtt aatttctcct tcttttacaa tgtaattttt ctcggatgga
    21181 tgaattaccc tcagatcctt agcatccagc tttccttttc ctctgagatc atccttggca
    21241 gcagtaattt gctggccaat aacagacttc tcgttgtcat atactatatc tatagccaga
    21301 aaaagctttc ggattgaatt ggcgatctta aggtgatggc tgtcaatata ctgccattga
    21361 tattcaatta catcgatttt atttttgtca aaaaattcac tcacaagatg catatcatct
    21421 ttcgatataa gtgaaaaaac cttttcacga acatatccaa aagggatgct gtcttcaatg
    21481 ttactatcaa cgaagaagtt aaatatttta ctcgcaaact taagcttttt atttattaga
    21541 tctatttcct ctgttgcccg agcttttgca aacaaaactg aagcgtcttt atgttttttt
    21601 actaaatatc tgaaggctgt gattatttta tcatttatct ctctgtatct gaagtacaag
    21661 tagcagcaca aataaagtat tccttgccat ttagggtgtc gcttaagtct gaatgttgta
    21721 ctgtgtttta cgatagttgc atagtactcc atgtttccct gggatatacc taatgaatta
    21781 attatatctt ttatttcata gtaaatacta cttatcacat tgtgagttct taattcattt
    21841 tctatttctg atgctgagaa gtctctcgtg ctataattat atcccctgat ggagtttaat
    21901 attccatcat catggatgat ggatgtaatc ttttgtgcag tggcaggata aattctcttt
    21961 gacaaaatat taattgtcct tttcctttca aagtttaaag catctgaaac gaggttctgg
    22021 acagtactgt atccaggaag agctatacgc cgctgtccta aaaaagcaag taattcatca
    22081 aaaatatatt ttggttccat acatatagta gcaacatcat tcagacgttc acttaactca
    22141 ttttttatag atggggtgag ttgttcaaat cctagagtag aaagcattcg tttaacaagg
    22201 cggtactttg tacttttagg tatgtttaaa ttgagattag tactttcgtt tttataatat
    22261 gtctggataa tatatttcac atcccttttt acgtctttta atgtaaattc aggaatcaca
    22321 ggtttatatc tgaaataccc tgttattaat atcagataca ccctgctttc tatatttatt
    22381 attttatcaa tttctctttt aaggctgata tccagcgaaa aaaactcatc cctttcaaca
    22441 ggattaaaag aggggacttg atataattca tttatttcac tttttgttaa tatactaatt
    22501 cttttcttgt acttctgcat acatttacca aaatgctaaa tatttgttaa atatggctag
    22561 tatcaaatat taagatatct gatacctgtc taaaacagca taaaagcagg tttcaaaaat
    22621 cgtattttgt acattatgat accatgcagt gtcaaaaacg ataagtgaga ctaaaaatgg
    22681 cgttattggg gtatgcacgg gtatcaacca gtcatcagaa gctaacacat caaatctcgg
    22741 agctaaaaaa tgccggggta cgggaagatc gtatttttac ggatatgatg actggcgcaa
    22801 ctgatgaacg ggaaggacta cagcggttgt tggcccgtgc agagaaagac gacgtaatca
    22861 tctgtacaaa aatggaccga ctggggcgta acactgctga catgattcgt gtggttgata
    22921 gctgttacaa aaaggggatc gccatccggt tcctggagaa cggtctcagt acagaaggga
    22981 caatgggcaa aatggttatc caaatccttg ctgctgtagc tgaagcggaa cgtgagcgta
    23041 ttctggagcg gacaaacgac ggaagaatag cggcacttgc cgcaggcgta aaatttggca
    23101 ggaagaagca tccacgaacg cctacagcgt tggagcttat atcacaggga gaatctctgg
    23161 gcagtgtgac agaaaaaaca ggtatctctc gatcgacgta cttcaggtta aaaagaacaa
    23221 taaaaaatga tgctaaaatt gcaacgtttt ataaataaat tcaattaatt acctaagcaa
    23281 ggtgagactt ttagaggaaa tgggcgcgaa ccccctaaag cgttacaaga catcagaaat
    23341 gctgcgcttt atttttgcta ccattgcata cgatcccgat ccagatttaa ccccgctgac
    23401 cgtgagacgt ttgtgcaagg cattgtttgg tagaagcgga agccagtggc tggtcgttga
    23461 agtttttggt gagaaggggc gtcagcaccg cagcgcggac agtaacccgg atatggtgga
    23521 aaaaatggca gccagatatc gtcatgccgc cggtcagcac tggtccgcaa cgctggctga
    23581 aattgaacgg gtaaagcgca tctaccaggc aagaatcaag gcatcctgag accggatgcc
    23641 tggtatttgt cagggccatt aatgctaatg tatgcataca ttagcattaa tggccacatc
    23701 ctggatgagc ttctacgctg gaactggggt ctgaagtcca atggaacgaa aacgtacgtt
    23761 agtgaagtaa ctgtctgata tatcaaaaca taatggaggc cctcaaaccc caactacagc
    23821 cgaacgacag gcacaaaaaa acccgactgg taatcgggct tttttgcatt ttccagtctt
    23881 gagttggcga ctctgaccgg agaaggttac tctacaccta acgtttacat tttattcact
    23941 atggtgctgt aaagcaacta aatgtggtta aggaaccaca tttagtggtg taacgggtgt
    24001 tttgtgtagc gtataatcag tcagttagtc atgtagttgg cttgcaatcc tgctcttccc
    24061 gtatctgcgg gtatgaacga ttttaaagtc ctggctggat aaacaggtgg tgctgtattc
    24121 ggtgctctgg caggaggagg tcactctaca cctaacaatg cgagagagcc gttgtgacag
    24181 gcaatgtatg agtaagcaac ggtgatttcc tccagtttta ctggagaaac ggatgattaa
    24241 tctgttaatc atcgttctca gggctgtggt cgccgttgca aacgcgctga ttgcaattct
    24301 ggaactgatt cgccagttca tcgactgatg acagcggaag cgtaactaag ggcaggaagt
    24361 gcaccgcttc ctgcccttta acaaaggagg atttatggat tttatgagca ttttagggcg
    24421 ctgtttactt cttcctcttc cggttgccat attcattggg tgcttacttt ctcaggacga
    24481 tgaccgattt tttggcatgt ttatgagtgt agtaagtgct gggttgtcgt ggtatatcac
    24541 taagccaaaa ccacaaaagg ctgaggtcgc gtaagcggcc ttttatttcg gtatttatta
    24601 gttacaacat tcacttttct tttttaaagc aacaccttag catcgcccca cccatcataa
    24661 ataatgtcag ccagtttatt gagagcgccc tgccctactc actggataat tgccgggatt
    24721 tacgttttat tgcgctactg attgtgccac ggctgcaccc tagcaccttc tgtacctgtg
    24781 tccaggagct accgctttct atcaacctga taattgcatc atgtctggcc tgattaggtt
    24841 tacgctcttt atatttgccg tctttccttg ctttctctat cccctgtttc tggcgttccc
    24901 gcagttgttc ataatccctt ctggcaacag cagccagcat atccagcagc atatcgttaa
    24961 tggctgaaaa caatcggttg tcaaactcac tcacactcaa aaagaaccag aaaacgtacc
    25021 cataaccaga aagtgacaat gtcatacata tgctttacga cataaagatg atgtaaagat
    25081 aaatacagct ttaccacgta acgcaaaaat aaagacgcaa atacctttac aaagtaaagg
    25141 tattataaag acactattat ctttatttgt gctttacatg gtaaagctta tgtaatataa
    25201 agacactgta aagacaaaac catctttaca aaaatcacac attgtaaaga caatagagtc
    25261 tttacacatt ctttacatca taaagagagg aattctgaaa tgggtaaaat cttgcttgtc
    25321 gtatcagata agggtggtgt aggcaaaagc acctacgtgg ctaatacagg ctcaatgctg
    25381 gtcaacaaag gtaagtcggt aatcatcctg aagacagata aaaaccacga tctgttgagc
    25441 tggaacgaaa agcggataga taacggctta ctcactatcc cggttcacgc agcttatggg
    25501 aacgtcagca acgaaattaa gcgccttagt aagctgtgtg aagtgctcat tgtcgattgc
    25561 cccggccacg acagccagga gttccgtagc gccctgacag tatcagatat cgtggtaaca
    25621 ctagttaagc cgtcctctga tttcgaaagc gaaacactga ccagtgttac agagaaaatc
    25681 cgcaccgctc agaaagctaa cgcagccctg gaaccgtggg tgctgttcac aagaatcaac
    25741 tcagctaaac cccgccacag aaaagcggcc attgatctgg ataaattact gcgctcggac
    25801 aacatctgga ttcaacctct gaaaaccagg atatccgagc tggatgtgta tgaaagcgcc
    25861 tgtaatgaag gggcaggggt acatgatgtt agccgggcat caagcctttc aacagcgaaa
    25921 gcacagattg aactcgtggc tcaggaaatt gggattttgt agtaatatca atatgttaaa
    25981 ttattatctt tacattgtaa agataatata aaggcggtat gatatttact atcaatacat
    26041 accgtactag gggaaatttc atggctttaa aactaaaccg tcctaacatc gatgaatctc
    26101 agcaaccagc aacaaacgca gaaaccgcgc gttttatctc cggcgctacc agagcgcctg
    26161 tagtgggtaa accaaagttg atgaatttca gactgccagc gtactttgag gatatcctgg
    26221 aagcggaggc gcagagaaca ggccagagca agacaaccgt tctaaaggca gcgctggccg
    26281 cattcgataa cctggacgag aacctgaaaa atcattggct acttgagtct gcaaagttag
    26341 gttaacaagt aattcgctta tttaggttta aaaaatgaga aagagagatg attattatga
    26401 cgggttaatt gctggaggta ctgtggcaat tgctgttgca acccttctga cactgttaat
    26461 tactgtattt gtcaacaata aggaaaattc cgagtttcat gaatggataa actcacactg
    26521 tgaaaaagaa aatgttgaaa acacttcatt taagtgtgat gacggggatg ggaatatata
    26581 tatcgtaaaa aaggaagcgt aattaagggc ggggagggac cattcctgtt aacgccctac
    26641 agtcgtgacg aatttcatct ggcgcgttcc tctgttgagg aagaacgaga tgaaatcgaa
    26701 ctggccagac tcatgattgt catacagagg cagtatcacg gaggtttcgg gaaacaatgg
    26761 tcatactgca tagttgcttc ggcagctgga tacagtgcca gcgtccgtaa atttcacgct
    26821 ggagtggaca ggctcattga tgttactcac cgcatcaggc gaacccagat tgaaaattta
    26881 cccagggaag atgtgctatc ccgttatgat cgtacaggga tgctcttcta tctcgaacca
    26941 ttgaatttga aattaatccg tataaggaaa actgaaatgg caactttgca ggaattaatc
    27001 gacctgacgc cagaacagga aaaagcgtgg aagcgccttg tgaaggctgt aaaggatttc
    27061 agggcagccg gaggaaagtt ttatagtgtc ctggacacgc tgagcgcata caacggcgag
    27121 cacgttgcca gcattgataa cgatacgggc taccacactg caagcgtcta tatgccaagc
    27181 attgatgcgc cagggctaac cagttgggct gatgattggc acggcatcac gctgaaagat
    27241 ggcgttgaag tggatgagga ctaacctatg actactatta ccaaagagcg actttttctg
    27301 attcagtcag tccccacata cttctttgtt ttaaacaacc ccgctgctcg cggcggggtt
    27361 gtttattaaa taagttaaga tttacgtggt gccgtataga tgactttata cacacgacta
    27421 acaggatgat tcatacacct tacaaaggtc ccgttcttct taaacgccac atctgcgttg
    27481 tcataaatgt catcggaatc attaagtccg catattgtcc gggcatcact cgcagaaaaa
    27541 ctgtccacgc tttgaagagg ggtgacagct ctgtgaccag ccagaagtgc tggatatgtg
    27601 tctgctgttt gccagacgat tttttcaagt aatacgcctg caatcccgat tagcgcaact
    27661 acaacgcccg ttctgataac tgttttcgct ttcattttaa cttaactgat caagacgctg
    27721 ttaactccat ggtcgcacct gctgctgggg acagtacatg tcagtactta ccaaacaggc
    27781 ttcaaaaaag caacagattc gctatatcgg aagtttcgac ggtttcggct ctcctccctg
    27841 tatgacgact gcgtgtgcag tgctggcttg gttgttcaaa acttcaacga aaacagtatg
    27901 aaaaaagaac atcaaaaaaa atatttatct ggaaattggt actaacacga tctgttattt
    27961 tgttttttta tcaatcggta gatctacgat ctatttgtgt gtttattttt agtgtaaaat
    28021 tctgtagaaa cggcttaata tagatctaag atctacatat tcgcatattt acagataaaa
    28081 gcttttgtgt aatatgttat tacgctatta aaagttgttt tgattctgcg caaaaaaaaa
    28141 cagggctacg gatacttcat gactgatcgg ggatccccga ggcggaggtg tgcatgacga
    28201 ctaggaaaag cggcttcaac tggtatgttc tagagttttg ttctgcacgt acagcacgag
    28261 tgttttctca tctggaaagg ttaggggtaa cattctggtg tcccctgact tgttcccatt
    28321 atcgtagatc agataaaaat tgtttcagac ggaaagaagt gccgctgttt cctggatatg
    28381 tgttcataaa gatcgatttc aataagaccc atagcacaac tgttacgtca gttccttggg
    28441 taaagtcatt tgtgtcattt ggtggtgagc ctgttcctgt ttgcgaggaa atcattctgg
    28501 gattgagaag aagagagact aattattggg gggaatattc tcctatacgc ggaatacctc
    28561 atgaattggc tgaaatcttg ttagaaaata atccgatgaa aagaagtttt ttatttt
//
LOCUS       DAZKJR010000059        28375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKJR010000059 DAZKJR010000000
VERSION     DAZKJR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.059"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..308
                     /gene="mcbA"
                     /locus_tag="ACHOWS_002550"
     CDS             48..308
                     /gene="mcbA"
                     /locus_tag="ACHOWS_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFQ0048104.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            573..2855
                     /gene="fiu"
                     /locus_tag="ACHOWS_002551"
     CDS             573..2855
                     /gene="fiu"
                     /locus_tag="ACHOWS_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFQ0048105.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIKNEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            2897..3574
                     /gene="ybiX"
                     /locus_tag="ACHOWS_002552"
     CDS             2897..3574
                     /gene="ybiX"
                     /locus_tag="ACHOWS_002552"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFQ0048106.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            3648..3914
                     /gene="ybiI"
                     /locus_tag="ACHOWS_002553"
     CDS             3648..3914
                     /gene="ybiI"
                     /locus_tag="ACHOWS_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFQ0048107.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            4179..4439
                     /gene="ybiJ"
                     /locus_tag="ACHOWS_002554"
     CDS             4179..4439
                     /gene="ybiJ"
                     /locus_tag="ACHOWS_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFQ0048108.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(4579..5664)
                     /gene="hcxB"
                     /locus_tag="ACHOWS_002555"
     CDS             complement(4579..5664)
                     /gene="hcxB"
                     /locus_tag="ACHOWS_002555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFQ0048109.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAVVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(5805..6767)
                     /gene="ybiB"
                     /locus_tag="ACHOWS_002556"
     CDS             complement(5805..6767)
                     /gene="ybiB"
                     /locus_tag="ACHOWS_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFQ0048110.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIKRCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            complement(6795..8945)
                     /gene="dinG"
                     /locus_tag="ACHOWS_002557"
     CDS             complement(6795..8945)
                     /gene="dinG"
                     /locus_tag="ACHOWS_002557"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFQ0048111.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            9049..9249
                     /locus_tag="ACHOWS_002558"
                     /pseudo
     CDS             9049..9249
                     /locus_tag="ACHOWS_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            complement(9484..10845)
                     /gene="rhlE"
                     /locus_tag="ACHOWS_002559"
     CDS             complement(9484..10845)
                     /gene="rhlE"
                     /locus_tag="ACHOWS_002559"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFQ0048112.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            11074..11745
                     /gene="cecR"
                     /locus_tag="ACHOWS_002560"
     CDS             11074..11745
                     /gene="cecR"
                     /locus_tag="ACHOWS_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFQ0048113.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11748..12743
                     /gene="hlyD"
                     /locus_tag="ACHOWS_002561"
     CDS             11748..12743
                     /gene="hlyD"
                     /locus_tag="ACHOWS_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFQ0048114.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12736..14472
                     /gene="ybhF"
                     /locus_tag="ACHOWS_002562"
     CDS             12736..14472
                     /gene="ybhF"
                     /locus_tag="ACHOWS_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0048115.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            14465..15598
                     /gene="ybhS"
                     /locus_tag="ACHOWS_002563"
     CDS             14465..15598
                     /gene="ybhS"
                     /locus_tag="ACHOWS_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0048116.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15609..16715
                     /gene="ybhR"
                     /locus_tag="ACHOWS_002564"
     CDS             15609..16715
                     /gene="ybhR"
                     /locus_tag="ACHOWS_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0048117.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16677..17087)
                     /gene="ybhQ"
                     /locus_tag="ACHOWS_002565"
     CDS             complement(16677..17087)
                     /gene="ybhQ"
                     /locus_tag="ACHOWS_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFQ0048118.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            17220..17981
                     /gene="ybhP"
                     /locus_tag="ACHOWS_002566"
     CDS             17220..17981
                     /gene="ybhP"
                     /locus_tag="ACHOWS_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0048119.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKMQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            17978..19219
                     /gene="clsB"
                     /locus_tag="ACHOWS_002567"
     CDS             17978..19219
                     /gene="clsB"
                     /locus_tag="ACHOWS_002567"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFQ0048120.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAMFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            19219..20175
                     /gene="ybhN"
                     /locus_tag="ACHOWS_002568"
     CDS             19219..20175
                     /gene="ybhN"
                     /locus_tag="ACHOWS_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFQ0048121.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(20211..20600)
                     /locus_tag="ACHOWS_002569"
     CDS             complement(20211..20600)
                     /locus_tag="ACHOWS_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048122.1"
                     /translation="MESYSQSSNKLDFQHEARILNGIWLITALGLVTTAGLALVIIRN
                     AALMSERPIWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVSCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(20806..21510)
                     /gene="ybhL"
                     /locus_tag="ACHOWS_002570"
     CDS             complement(20806..21510)
                     /gene="ybhL"
                     /locus_tag="ACHOWS_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0048123.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21647..22099)
                     /gene="moaE"
                     /locus_tag="ACHOWS_002571"
     CDS             complement(21647..22099)
                     /gene="moaE"
                     /locus_tag="ACHOWS_002571"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFQ0048124.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(22101..22346)
                     /gene="moaD"
                     /locus_tag="ACHOWS_002572"
     CDS             complement(22101..22346)
                     /gene="moaD"
                     /locus_tag="ACHOWS_002572"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFQ0048125.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(22339..22824)
                     /gene="moaC"
                     /locus_tag="ACHOWS_002573"
     CDS             complement(22339..22824)
                     /gene="moaC"
                     /locus_tag="ACHOWS_002573"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFQ0048126.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22827..23339)
                     /gene="moaB"
                     /locus_tag="ACHOWS_002574"
     CDS             complement(22827..23339)
                     /gene="moaB"
                     /locus_tag="ACHOWS_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFQ0048127.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(23361..24350)
                     /gene="moaA"
                     /locus_tag="ACHOWS_002575"
     CDS             complement(23361..24350)
                     /gene="moaA"
                     /locus_tag="ACHOWS_002575"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFQ0048128.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(24338..24482)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24747..25655
                     /gene="yvcK"
                     /locus_tag="ACHOWS_002576"
     CDS             24747..25655
                     /gene="yvcK"
                     /locus_tag="ACHOWS_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0048129.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(25847..27868)
                     /gene="uvrB"
                     /locus_tag="ACHOWS_002577"
     CDS             complement(25847..27868)
                     /gene="uvrB"
                     /locus_tag="ACHOWS_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0048130.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         6725 a         7617 c         7332 g         6701 t
ORIGIN      
        1 acctacactt atctggcact acccgttgta tttatgagga tgtcattatg aaaaagtgcc
       61 tcacactact gattgccacc gtcctgagcg gaatctctct cacggcttat gccgcgcaac
      121 cgatgagtaa cctggacagt ggtcaactac ggcccgccgg taccgtttcg gcaactggcg
      181 catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa caaggcgcga
      241 aaggttatgt cattaattcc gcaggcggaa atgaccagat gttcggtact gcaatcatct
      301 acaaataacc ccaccaacgt aactgcattc gcctctggat gcagttttcg tccaatttgt
      361 aagcaaaaaa catcaaatat gaacgcaatg taaataaatg tatttctttt tcgcgcaatg
      421 ggtgatagaa aatcgcgcca agtgataatg cttatcaaaa ttattatcac ttgcacggac
      481 gctatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt gacagtgaaa
      541 atttcaccat ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat ttccctgcca
      601 gacaatttca ttcgctcacg ttctttgccg gtctttgtat tggtattacg cctgtggcgc
      661 aggcactcgc tgccgaaggg caagctaacg cggatgacac gctggttgtc gaagcatcaa
      721 cgccttcgct ttatgcgcca caacaatctg ccgatccgaa attctcgcgt ccggtagcgg
      781 atactacccg cacgatgacg gtgatttctg aacaagtgat taaagatcag ggtgcaacca
      841 accttaccga cgcgctcaaa aacgtccccg gcgtgggtgc gttttttgcg ggtgagaacg
      901 gtaactccac cactggcgac gccatttata tgcgcggtgc cgatacctct aacagtattt
      961 atatcgatgg cattcgcgat atcggcagcg tctcgcgcga caccttcaat accgagcagg
     1021 tcgaagtaat taaagggccg tccggcaccg actacgggcg cagcgcgccg acaggctcga
     1081 tcaatatgat cagcaaacag ccgcgcaatg attccggcat tgacgcctcc gccagtattg
     1141 gcagcgcctg gttccgccgc ggcacgctgg acgtcaatca ggtcattggt gataccactg
     1201 cggtgcgcct gaatgtaatg ggcgaaaaaa cgcacgatgc cggacgcgac aaagtcaaaa
     1261 atgagcgtta cggcgtcgcc ccttctgtcg cttttggcct tggtacagcg aatcgtttgt
     1321 atcttaatta tctgcatgtc acccagcaca acacgccaga cggcggcatt ccgactatcg
     1381 gtttaccggg ttattctgcc ccatctgcgg gaacagcggc tctgaatcat tccggaaaag
     1441 ttgatactca taacttttac ggcacggatt ccgattacga cgattcgacc accgacaccg
     1501 ctaccatgcg ttttgagcac gacatcaacg ataacaccac cattcgcaat actacccgtt
     1561 ggtcgcgcgt gaagcaggat tacctgatga cggcgattat gggcggggcg tcgaatatta
     1621 cccagcccac cagcgatgtg aatagctgga cctggtcacg cacggcgaat actaaagatg
     1681 tgagtaataa aattctcacc aaccagacca acctgacctc gacgttctat accggcgcta
     1741 tcggtcatga tgtcagtacc ggcgtggaat ttacccgtga aacgcagact aactacggcg
     1801 ttaatccggt gacgttacct gcggtaaata tttatcatcc tgacagcagc attcatcccg
     1861 gcggcctgac gcgcaacggc gcaaacgcca atggtcagac ggataccttc gcaatttacg
     1921 ccttcgatac gctgcaaatc acccgtgatt ttgagctgaa cggcgggatc cgtctggata
     1981 attatcatac tgaatatgac agtgccaccg cctgcggcgg cagcggacgc ggtgccatca
     2041 cctgccctgc tggcgtggca aaaggttctc cggtcaccac cgtcgatacc gccaagtcgg
     2101 gcaatctggt gaactggaaa gccggggcgc tgtatcacct gacggaaaac ggcaatgtct
     2161 atattaacta tgccgtttcc cagcagcctc cgggcggcaa caacttcgcc cttgcgcagt
     2221 ctggcagcgg taacagtgcc aaccgcaccg attttaaacc gcaaaaagcc aacaccagcg
     2281 agattggcac caaatggcag gttctggata aacgcctgtt gctcaccgcc gcgctgttcc
     2341 gtactgatat caaaaatgaa gttgagcaaa atgatgacgg aacttactcg caatacggta
     2401 agaaacgcgt cgaaggctat gagatatccg tggccgggaa tatcactccc gcgtggcagg
     2461 tgattggcgg ctatacccag caaaaagcaa ccatcaaaaa cggcaaagat gttgcccagg
     2521 atggttcctc atcgctgccg tataccccgg agcacgcctt taccttatgg agccagtatc
     2581 aggcaaccga tgatatttcc gttggtgcgg gcgcacgcta tatcggcagt atgcataaag
     2641 gttctgacgg cgcggtggga acgccagcgt ttaccgaagg ttattgggtc gccgacgcta
     2701 aactggggta tcgggttaat cgcaatctcg acttccagct aaacgtctac aacctgtttg
     2761 ataccgatta cgtcgcctca atcaacaaga gcggctaccg ttatcacccg ggcgagccaa
     2821 gaaccttctt gctcacggcc aatatgcatt tctgattgag atgtggggcg caggccccac
     2881 tttttggaga acttgtatga tgtaccacat tcccggcgtg ctatcgccac aggacgtcgc
     2941 ccgttttcgc gaacaactgg aacaagccga atgggtggat ggacgcgtca ccactggcgc
     3001 acaaggtgcg caagttaaga acaatcaaca ggtagacacc cgcagcacgt tatacgccgc
     3061 cctgcaaaat gaggtgctga acgcggttaa ccaacatgct ttgttctttg ccgcggcctt
     3121 gccgcgtacc ctttccacgc cgctgtttaa tcgctatcag aacaatgaaa cctatggttt
     3181 tcatgtggat ggcgcagtac gcagtcaccc acaaaacggc tggatgcgca ctgacctttc
     3241 tgccacgctg tttttaagcg atccacaaag ctacgatggc ggcgaactgg tcgttaatga
     3301 caccttcggg caacatcggg taaaacttcc ggcaggcgat ctggtgttgt atccctccag
     3361 cagcctgcat tgcgtgacac ccgtaacccg cggcgtacga gtggcatcat ttatgtggat
     3421 ccagtcgatg atccgcgatg ataaaaagcg cgccatgctg tttgaactgg acaacaatat
     3481 tcagtcgctg aaaagccgct acggcgaaag tgaagagatc ctgtcgctgc tcaatcttta
     3541 tcataatctg ctgcgggaat ggtcggagat ctgacgcttg aactgcccga gttcgtccct
     3601 atattagcta cacttaactg tacaagtatt gatatgggga ggacgatatg gcatccggtt
     3661 gggctaacga tgacgccgtc aacgaacaga tcaacagtac aattgaagat gcgattgccc
     3721 gcgctcgggg tgaaattccg cgtggcgaaa gcctgtatga atgtgaagag tgcggagccc
     3781 ccatcccgca agcccgtcgg gaagccattc ctggtgtgcg cttatgcatt cattgtcagc
     3841 aggagaaaga tttacaaaaa ccagcttata caggatataa tcgcagaggt tcgaaagaca
     3901 gccagttacg ttaactacaa ctggcgggat gtatagcgag tataatttgt attttgcgtg
     3961 gcattccgtg cctttaacgc tgtcgtgact gccacgctgc ataaaacaag tccataagtt
     4021 caacaaataa atggattatc agtatgttag atatcaatca atttatttga acaaggcggt
     4081 caattctctt cgattttatc tctcgaaaaa aaacgtgata ctcatcacat cgacgaaaca
     4141 acgtcactta tacaaaaatc acctgcgaga gattaattat gaaaactatc aatactgttg
     4201 ttgctgctat ggctctttca actctgtcat ttggcgtgtt tgccgcggaa ccggtaacgg
     4261 catcccaggc acagaacatg aataaaatcg gcgtggtttc tgccgatggc gcatccaccc
     4321 tcgatgccct ggaagcgaaa ctggctgaga aagctgcggc agctggggcg agtggataca
     4381 gcatcacttc tgccaccaac aacaataaat taagcggtac tgcggtaatt tataagtaat
     4441 tcgtagaacg gataaggcgt ttacgctacg tccagcacaa gtatttccct gtctgtttgc
     4501 cgacagacgc atatgctcta accctcattg atcctatgtt acccttgttt gcccgtccgc
     4561 cactggacgg gctttttttt agctggctaa ctgctgacag aaagcctgca acgtctcttc
     4621 cggcatacca acctttcgcg ccgcatcaca aatggcctgc cagcttcccg catccaatgg
     4681 aatcccctgc tcctgccgtt cgcgacgcgt gttaacttcc cactcgcccg gtagcaaaat
     4741 cggcttatcg tcatcatgcg gcgaggcttt cacccactcg gcaaaggctt cggtctgcgc
     4801 gctacaatcc ggcgcgccga acagttccgg gttgatgatg atagtggtca tgcagttaag
     4861 aatggcatcg ggactggttt gtaacgtttt ctgatgcgtc gttttaccgc cggaaagcgc
     4921 cccaccaaga atttcacaca tcgctgcaag ggcgtagcct ttatgttcgg caaaggtcaa
     4981 cagcgaaccc aacggcgact cctgcattac cgccggattg gtcgtcggca tgccgttaac
     5041 gtcaatcagg caacctggcg gcacggggac gcctttatgc caggcgacgc gggttttgcc
     5101 aaatgcgatg gcgctggtgg cgtaatcaag caacagcggg aaattatctt tacgagggaa
     5161 aaccacacag aacggattgg tgccaaagcg gctgtcgcga ccgtggaacg gcgcgaccat
     5221 tggaataccg accacgctaa caaagtggat agagacaaac cccgccgctg cacactgctc
     5281 cgcccagtaa ccgatacggc cgatatgatg cgagttatgt agcgccacgg cagcaatacc
     5341 gtgctgatgc gctttctcaa tccccagcgc catcgcttcg tgtgccgcga cctgaccaaa
     5401 tgcgcgatcg ccatcaagcg taaccaccgc gcccgcttct ttaacgattt tggcatggtg
     5461 gttaatttgc agatgcccct gactccagga acgcacatag cttgggatca tgccaatacc
     5521 atgtgaatca tgccctgcca ggtttgccgc gattaaatga tcggcaacta atttcgcttc
     5581 ttgttcctcg ctacccatct gacgaaatac agcctgaata aaactgtgca gcgtctgagc
     5641 atcaaagcga tgaccacttt ccatggttta ctcctgttta tgttatgtgt ttgttgtttt
     5701 tttatgctgc acgccgggcg cgggaaggtc aataaagatg caggaatttc atattgcggt
     5761 ggggtaaaag ccggatgaca tggctcatcc ggctgagata aaaattaaaa tgcctgatta
     5821 atgcgcgcca ggccgtcgtt gatagttgcc gcttcacccg tagccatcag gcagcaagcc
     5881 atctggattt tcagcgattc aggaatcggt tcgctgccag caaggcaacg cttaatccac
     5941 tgcgccgtgg tttccggatc ttttgcttgt ggcagtaact cgctaccagc agtgtcctgt
     6001 ttttcataca gtacccgcat cccttcacgg tcaatgagat tgatctgcgg gcagcgctgc
     6061 ggattagcat acacttcacc ttcagtacca tgcattagta gcgcccgccc gccgatatca
     6121 ctaaagaact tcgcgacgcg tccaatgtat tccggatgcg aaacgctgga aagacgcagt
     6181 gcctcacctt cggcaaatgg cgtcgccagt ttcgccaggg tatgtgcact gttacgcacg
     6241 cccatccgcc agcgcatcgc cagttgtttt tccagcggcg ggcaaaacgc gccgacaggc
     6301 ataaacaccg gttgatgttc gtcgagcttc gcctgcgcct gcccgccgtg caacgttggc
     6361 gtaataccca tcagttcgaa aatggtttct gtcagcacgc gggttggatc ttcgctaacc
     6421 ccgtgaacca ccacaggaaa accgagtttg tgcaggagaa tcgccaacaa cggcgtcagg
     6481 ttggcctgtt tacgcgcgcc gttgtaactg gggatgacaa tcggcatcgg cttgtctgca
     6541 ggcggcgtca gcttgatggt gtgattttgc atggcttcgt aaaagccgag catctctgct
     6601 tccccttccc ctttgatacg cagcgcaatc aatacgccgc ccaactcaag gtcagggact
     6661 tcaccattga gcatatgggc atacagaccg cgcgcagtat cccggtctaa atcgcgcgcg
     6721 tggtttttcc cgcgcccgat ctctttaatg attttgcgat agtccattta cgactcctta
     6781 cctgactcac atcattaacg ccgccgacgg cgtggggatt tcgttttttc tttcttttta
     6841 actatacctt cagggacttc cggttgctct atcggaaata ccggtaatgc atccagtagt
     6901 cgcttgccat aatttttggt cagcaagcgt ttgtcgtaga taaccacttc cccccagcaa
     6961 ccgtggcttc gaatcagtcg cccaacctgc tgaatcaggt taaacgaagc gctcggcagg
     7021 ctttgcacct caaacggata gcggttgagg cttttcagcc attcaccttc ggtgatcacc
     7081 accggactgt cgatgggcgg aaaagcgatc ttgtggatat gcacctgact gagcagatcg
     7141 cctttcagat caagaccttc ggcaaaggac tgtaagccca ccagcacgct acgctcaccg
     7201 ttggcaacgc gtttgcggtg cagttcaact aagcgataac gtggctgatc gccctgcacc
     7261 agcaacatca ggcgtaaatc cgtcacatag tcgagaaagc gctgcatcgc acgcccgctg
     7321 gcaaacagta ccaacatacc gagatgtttt ttgctctcca cctgctcacg gaaaaaggct
     7381 gccatttcag caatgtgctg ctcttcgttg tcgatggaag gctcaaagcg catccgggga
     7441 ataacaattt tgccctgttc gcagtggtta aagggcgaat ccagcgccac aaagcggtcg
     7501 cccgctttct ctttcagacc actcatctcc tgtaaacgcg aaaaactgtt cagcgaacgt
     7561 aaggtcgcgg aagtgacaat aatgtgcgga atactgcgcc acagcagcct ttccagctga
     7621 tcgctgacac gaatacccac acagtgaaac cagagatgca gctgcccttc gcgctcttcc
     7681 cgcgtcgccc atttagtcac cggcgcaccg gaagattgcg ccagagacgc cagccgccag
     7741 agtttgcttt gcgcctcgaa catccccaac gcgcgattca tctgcaaaat caaccgatgc
     7801 agacgtacaa tgtcatggct accggttttc tcacttaaat cgttaagaaa taactccgcc
     7861 aggccacgca gcatctcggt gagttttgcc agccgctggc agatctccag cacttcatca
     7921 ggcagttcgc ccatcgcaaa acggtgctct gcctcctgcc cggcaggcat gtagagattg
     7981 agaatgttgt ttaacgaggc gataagctca tacagctctt cacaatgcgc attcaaacgt
     8041 tcagggatcg ccagcggtgg gatggtcttc gggcgaaact gctccatgca ggtagcgacc
     8101 agtttcgtga acaagtccag ctgtagccga taccagggcg cggtgatttc ggcgctcatc
     8161 tccagcgcat cccgcgccac atctggcagg tgatgacctt cgtccagcac cagcagcaga
     8221 tttttcgggt caggtagtac ggcttcgctt tccatcgccg ccatcaccag cgcatggttt
     8281 gccaccacca cttccgcttc ctgaatctcc cgacgagcaa caaaaaacgg gcattcacga
     8341 tagtagtaac agttgcggtt gaggcagctg gctttgtcgg tacttaaacg acgccagaga
     8401 tcgtcatcaa tggcgatatc cgtatgatcg cgcagaccat cccatttata agtgtcgaga
     8461 tcgcccttca gcttcgcaca acgtttttgc tcttcctgat tgttcggcgt cagctcgtcg
     8521 tcaagaaacg ccagcagatc ctgttgcgtg ggttcagtac tggcaagcgc tgtcagatta
     8581 cgcggacaaa cgtagcgccc acgcccaaaa gcagcagtga atttaagatc gggaatgatc
     8641 tttttcagca gaggcaaatc tttactgtag atctgatcct gcaatgccac gttggcggta
     8701 ctcaccacca gcgttttttg ctcttcgcgg gcaatggcga tgccgggaat caaataggag
     8761 agcgttttcc caacgccggt gggtgcttca atcgccagat gccgcccttc ttctccggcc
     8821 agcgttttgg cgacgtccgc aatcatctgc cgctgcggcg cacggggaat aaagtcgggg
     8881 atctgttcct gaagcgcctt ataccaggcg gcaatttgcg ctttaagcgc ggcggttaat
     8941 gccatgagaa aacctgaaat actgtataaa cagccaatat tgtggcattt ttcggttttc
     9001 aggggtaatg gtttttctgt ttctggaaag cggctcgcaa taaccagcag gatgatgcgt
     9061 aaagcgcttc gcgctaaatt cgaacaacat gcagaactgc gtacactctt gctggcaacc
     9121 gcgtccgcaa aactggttga gcatacgcaa aatgatgctt actggggaga tagcggtaat
     9181 ggtcagggca agaatcgcct ggggtacctt ttaatggcgt tgcgcggaca attggctgcg
     9241 gagaagtaac gcatcgggtg gcttgtgggc gttaaaacag agaaatcttt ttgccgcgct
     9301 cgcaaatggc tgacaagtgc ttgttgtgaa atgccggatg cggcgtaaac gccttatccg
     9361 gcctacaaaa gcatgcaaat tcaatatatt acagagttca cgtaggcctg ataagcgcag
     9421 cgcatcaggc agttttgcgt ttgttcatca gcctgatgcc gggcatcgcc cggcatcaaa
     9481 agattactgc gcagcggcag gtttacgtgg acggcggcga cgttgttgtt cgcctgccgg
     9541 tttggcatcg ccgaggcggc gagacggttt ttctgcaggt ttcgcacttg cagattttgc
     9601 gccaccttct ccacggcgtg gttgctgttg accgcgacca ccaccttgcc cacgaccgcc
     9661 gccacgttgc tggcgaccgt tctggatcgg ttcggctttg attgacgggt ccggctcata
     9721 acccggaatc gcaatgcgcg ggatctcttt tttcagcagt ttttcgatat cacgcagcag
     9781 tttgtgttca tcaacacaca ccagcgacaa cgcttcaccg gtagcagccg cacgaccggt
     9841 acgcccgata cggtggacat aatcttccgg tacgtttggc agttcatagt tgaccacgtg
     9901 cggcagctct tcaatatcca ggccgcgcgc agcgatgtca gttgccacca gtacacgaat
     9961 atcgcccgat ttaaaatcag ccagcgcacg agtacgcgca ccttgcgatt tattaccgtg
    10021 gatcgccgca ctacggatgc catctttatt aagctgttca gccagatggt tagcgccatg
    10081 tttggtacgg gtaaacacca gcacctgctg ccagttccct ttcccaatca tatgcgacag
    10141 caattcgcgt ttgcgtttct tatcgacaaa gtgaacgtgc tgggtcacct gatcagacgc
    10201 ggtattgcgg cgtgccactt cgatttccag cgggttgtgc aacagttttt ccgccagggc
    10261 tttaatatcg tcagagaagg tcgcggagaa caacaggttc tggcgcttcg ctggcagttt
    10321 tgttaacacg cgacggatat cgtggataaa gcccatgtcg agcatgcggt cagcttcatc
    10381 aaggacgagg atttcaacct gatccagctt cactgcattt tgatgttcca ggtccagcag
    10441 acgtcccggc gttgccacca gcacatcaac gccgccgcgc aacttcatca tttgtgggtt
    10501 aatgctgaca ccgccaaaca ccaccagcga acgaatgttc agatatttgc tgtaatcacg
    10561 gacgttttcg ccaatctgcg ccgccagttc acgggtcggg gtaagaatga gcgcgcgtac
    10621 cggacgacgc cctttggcgt gcggctggcg agtgatcagg tgttgcaaca gcggcagcgt
    10681 aaagcccgct gttttgccgg taccggtctg ggcgctggcc atcaggtcgc ggccttccag
    10741 caccgcaggg atcgcctgct gctgaatagg ggtgggttca cggtaaccct gctcggcaac
    10801 ggcgcgcagg atatcagggc ttaaacccaa agaatcgaaa gacataacta ctccgaaccg
    10861 ccccgaccgt taccggtgta gttttcaggg agatactgaa aaggaaaatg acaaaaacca
    10921 caatgtcatg agggagcgga gtgtagcagt ttttgtgacg cagcgcataa attatccctg
    10981 tgactggcgt tgctaaaatt cttagccata ctggacaact ctcatatgga gtcataatct
    11041 taatcaatca tttgattaag aatggactgt gcgatgaata atcctgccat gacaatcaag
    11101 ggtgaacagg cgaagaaaca gctgattgct gccgcactgg cgcagtttgg tgaatatgga
    11161 atgaacgcca ccacccgcga gatagccgcc caggccgggc agaatatcgc tgccatcacc
    11221 tattacttcg gttcgaaaga ggatttgtac ctcgcctgcg cccagtggat tgccgatttt
    11281 attggcgagc agttccgtcc acacgccgaa gaagccgaac gtttgttcgc gcaaccacag
    11341 cctgatcgcg cagctatccg tgaactgatc cttcgcgcct gcaggaacat gattaagctg
    11401 ctcacccagg atgacaccgt caacctcagc aaatttatct cccgtgagca gctctctccc
    11461 acggcagcct atcacctggt gcacgaacag gtgattagtc cgctacacag ccaccttaca
    11521 cgcctgattg ccgcctggac cggctgcgac gctagcgata cccgcatgat ccttcatacc
    11581 catgcgttga ttggtgagat tctggcgttt cgtcttggca aagaaactat tctgttacgt
    11641 accggctgga ccgcgttcga tgaagaaaag accgaactga tcaaccagac ggtgacctgt
    11701 catattgacc tgattttgca aggattatcg caaaggagtt tgtagtgatg aaaaaacctg
    11761 tcgtgatcgg actggcggta gtggtacttg ccgccgtggt tgccggaggc tactggtggt
    11821 atcaaagccg ccaggataac ggcctgacgc tgtatggcaa cgtggatatt cgtacggtga
    11881 atcttagttt ccgtgttggg gggcgcgttg agtcgctggc ggtggacgaa ggtgatgcga
    11941 tcaaagcggg ccaggtactg ggcgaactgg atcacaagcc gtatgagatt gccctgatgc
    12001 aggcgaaagc gggcgtttcg gtggctcagg cgcagtatga cctgatgctt gccgggtatc
    12061 gagatgaaga aatcgctcag gccgccgcag cggtgaaaca ggcgcaagct gcctatgact
    12121 atgcgcagaa cttctataac cgccagcaag gactgtggaa aagccgcacc atttcggcaa
    12181 atgacctgga aaatgcccgc tcctcgcgcg accaggcgca ggccacgctg aaatcagcgc
    12241 aggataaatt gcgtcagtac cgttccggta accgcgaaca ggacatcgcc caggcgaaag
    12301 ccagcctcga acaagcgcag gcgcaactgg cgcaggcgga gctgaattta caggactcaa
    12361 cgttgatagc cccgtctgat ggcacgctgt taacgcgcgc ggtggagcct ggcacggtgc
    12421 tcaatgaagg tggcacagtc tttaccgtct cactgacacg tccggtgtgg gtacgtgctt
    12481 atgttgatga acgtaatctc gaccaggccc agccggggcg caaagtgctg ctttataccg
    12541 acggtcgccc ggacaaaccg tatcacgggc agattggttt cgtttcgcct accgctgaat
    12601 ttaccccgaa aactgtcgaa acgccggatc tgcgtaccga cctcgtctat cgcctgcgta
    12661 ttgtggtgac cgacgccgat gatgccttac gccagggaat gccggtgacg gtgcaattcg
    12721 gtaacgaggc aggacatgaa tgatgccgtt attacgctga acggtctgga aaaacgcttt
    12781 ccgggcatgg acaagcccgc cgtcgcgccg ctcgactgta ccattcatgc tggttatgta
    12841 acggggctgg tggggccgga tggcgcaggt aaaaccacac tgatgcgcat gctggcggga
    12901 ttactgaaac ccgatagcgg cagcgccacg gtgattggct ttgatccgat caaaaacgac
    12961 ggcgcgctgc acgccgtgct cggctatatg ccgcagaaat tcggcctgta tgaagatctg
    13021 acggtgatgg agaacctcaa tctgtacgcg gatttgcgca gcgtcaccgg tgaagcgcgg
    13081 aagcagactt ttgctcgcct gctggaattt acatctcttg gcccgtttac cggacgcctg
    13141 gcgggcaagc tctccggcgg aatgaagcaa aaacttggcc tggcctgtac cctggtgggc
    13201 gaaccgaaag tgttgctgct cgatgaaccc ggcgtcggcg ttgaccctat ctcacgccgc
    13261 gaactgtggc agatggtgca tgagctggca ggcgaaggaa tgttaattct ctggagcacc
    13321 tcgtatctcg acgaagccga acagtgccgt gacgtgttac tgatgaacga aggcgagctg
    13381 ctgtatcagg gagaaccgac ggccctgact caaactatgg ccggacgcag ctttctgatg
    13441 accagcccgc acgagggcaa ccgcaaactg ttgcaacggg cattgaaact gccgcaggtc
    13501 agcgacggca tgattcaggg gaaatcggta cgtctgatcc tcaaaaaaga gaccacgcca
    13561 gacgatattc gccatgccga cgggatgccg gaaatcaaca tcaacgaaac tacgccgcgt
    13621 tttgaagatg cgtttattga tttgctgggc ggtgccggaa cctcggaatc gccgctgggc
    13681 gcaatattac atacggtaga aggcacaccc ggcgagacgg tgatcgaagc gaaagagctg
    13741 accaaaaagt ttggcgattt tgccgccacc gatcacgtca actttgccgt caaacgcggg
    13801 gagatttttg gtttgctggg gccaaacggc gcgggtaaat cgaccacctt taagatgatg
    13861 tgcggtttgc tggtgccgac ctctggtcag gcgctggtgc tgggcatgga tctgaaagag
    13921 agttccggta aagcgcgcca acatctcggc tatatggcgc aaaaattttc gctctatggc
    13981 aacctgacgg tcgaacagaa tttacgcttt ttctctggtg tgtatggctt acgcggtcgg
    14041 gcgcagaacg aaaaaatctc ccgtatgagc gaggcgttcg gcctgaaaag tatcgcctcc
    14101 cacgcgaccg atgaactgcc attaggtttt aaacagcggc tggcgctggc ctgttcactg
    14161 atgcatgaac cggacattct gtttctcgac gaaccgacgt ccggcgttga tcccctcacc
    14221 cgccgtgaat tttggctaca tatcaacagt atggtagaga aaggcgtcac ggtgatggtc
    14281 accacccact ttatggatga agcggaatat tgcgaccgca tcggcctggt gtaccgcggg
    14341 aaattaatcg ccagcggcac accggacgat ttgaaagcgc agtcggccaa cgatgagcaa
    14401 cccgatccca cgatggagca agcctttatt cagttgatcc acgactggga taaggagcat
    14461 agcaatgagt aatccgatcc tgtcctggcg tcgcgtgcgg gcgctgtgcg tcaaagagac
    14521 gcggcagatc gttcgcgatc cgagtagctg gctgattgcg gtagtgatcc cactgctact
    14581 gctgtttatt tttggatacg gcattaacct cgactccagc aagctgcggg tcgggatttt
    14641 actggaacag cgtagcgaag cggcgctgga tttcacccac accatgaccg gttcgcccta
    14701 catcgacgcc accatcagcg ataaccgtca ggaactgatc gccaaaatgc aggcggggaa
    14761 aattcgcggt ctggtggtta ttccggtgga tttcgccgaa cagatggagc gcgccaacgc
    14821 caccgcaccg attcaggtga tcaccgacgg cagtgagccg aataccgcta actttgtgca
    14881 ggggtatgtc gaagggatct ggcagatctg gcaaatgcag cgagcggagg ataacgggca
    14941 gacttttgaa ccgcttattg atgtacaaac ccgctactgg tttaacccgg cggcaataag
    15001 ccagcacttt attatccccg gtgcggtgac cattatcatg acggtcatcg gcgcgattct
    15061 cacctcgctg gtggtggcgc gagaatggga acgcggcacc atggaggcgt tactctctac
    15121 agagattacc cgcacggaac tgctgctgtg taaactgatc ccttattact ttctcgggat
    15181 gctggcgatg ttgctgtgta tgctggtgtc agtgtttatt ctcggcgtgc cgtatcgcgg
    15241 ttcgctgctg attctgtttt ttatctccag cctgttttta ctcagtaccc tggggatggg
    15301 gctgctgatt tccacgataa cccgcaacca gttcaatgcc gctcaggtcg ctctgaacgc
    15361 ggcttttctg ccgtcgatta tgctttccgg ctttattttt caaatcgaca gtatgcccgc
    15421 ggtgatccgc gcggtgacgt acattattcc cgctcgttat ttcgtcagca ccctgcaaag
    15481 cctgttcctc gccgggaata ttccagtggt gctggtggta aacgtgctgt ttttgatcgc
    15541 ttcggcggtg atgtttatcg gcctgacgtg gctgaaaacc aaacgtcggc tggattaggg
    15601 agaagagcat gtttcatcgc ttatggacgt taatccgcaa agagttgcag tcgctgctgc
    15661 gcgaaccgca aacccgtgcg attcttattt tacccgtgct gattcaggtg atcctgttcc
    15721 cgttcgccgc cacgctggaa gtgaccaacg ccaccatcgc catctacgat gaagataacg
    15781 gcgaacactc ggtagaactt actcaacgtt ttgcccgcgc cagcgccttt acccatgtgc
    15841 tgctgttgaa aagcccgcag gagatccgcc caaccatcga cacacaaaag gcattactcc
    15901 tggtgcgttt cccggctgac ttctcgcgca aactggatac cttccagacc gcgccgttgc
    15961 agttgatcct cgacgggcgt aactccaaca gtgcgcaaat tgccgccaac tacctgcaac
    16021 agatcgtcaa aaattatcag caggagctgc tggaaggaaa accgaaacct aacaacagcg
    16081 agctggtggt acgcaactgg tataacccga atctcgacta caaatggttt gtggtgccgt
    16141 cgctgatcgc catgatcacc actatcggcg taatgatcgt cacttcactt tctgtcgccc
    16201 gcgaacgtga acaaggtacg ctcgatcagt tactggtctc accgctcacc acctggcaga
    16261 tcttcatcgg caaggccgta ccggcattaa ttgtcgccac cttccaggcc accattgtgc
    16321 tggcgattgg tatctgggcg tatcaaatcc ccttcgccgg atcgctggcg ctgttctact
    16381 ttacgatggt gatttacggt ttatcgctgg tgggattcgg tctgttgatt tcatcactct
    16441 gttcaacaca acagcaggcg tttatcggcg tgtttgtctt tatgatgccc gccattctcc
    16501 tttccggtta cgtttcgccg gtggaaaaca tgccagtatg gctgcaaaac ctgacgtgga
    16561 ttaaccctat tcgccacttt acggacatta ccaagcagat ttatttgaaa gatgcgagtc
    16621 tggatattgt gtggaatagt ttgtggccgc tactggtgat aacggccacg acagggtcag
    16681 cggcgtacgc gatgtttaga cgtaaggtga tgtaacttct tatctttcgc cagcaaagac
    16741 actaccgccg ggccagcaag gattgccagc cctgcaatcg ccagcaaaaa gttgttttcc
    16801 agtacgcggg acaacagcca cagcacaatc agcgctgcgc caaaatacca ggtcgtggtc
    16861 agctcttcca gcacgtctgc cacctcacgc cagcgttgct cggggtggcg ctgaaacacc
    16921 agcatcatca ctaccgtgac acaatacagt gcgcataatc ccacgccgac gtgaaccaga
    16981 gtcttactca tccagcccac caccagcagc gccactacca gtaaatgcaa cataatctgc
    17041 cagcaactta gacccgttgc gacacgaaca cgttgttgcc acttcatggc ttctctcctg
    17101 aagaatttat ctctacttac tcagagtacc gaactttacg gaaaattccg taagaccgca
    17161 cctttttgtg acgccgacta cactatttca atgagataag aggcaaagac aggaaaatta
    17221 tgcccgatca aacacaacaa ttttcgttca aggtgctcac catcaatatt cacaaaggct
    17281 ttaccgcgtt taaccgacgc ttcattttgc cggaacttcg cgacgccgtg cgtaccgtca
    17341 gcgccgatat tgtttgcctg caggaagtga tgggcgcgca cgaagttcat ccgctgcatg
    17401 tggaaaactg gcccgatacc tcgcactacg agtttctcgc cgacaccatg tggagcgatt
    17461 tcgcctacgg tcgcaatgcc gtatacccgg aagggcatca cggcaacgcg gttctttcac
    17521 gttatcccat tgaacattat gagaatcgcg atgtttcggt cgatggtgcg gaaaagcgcg
    17581 gcgtgctcta ctgccgcatt gtgccgccga tgaccgggaa agcgattcat gtgatgtgcg
    17641 tacatctggg cctgcgtgag gcgcaccgtc aggcgcagct tgcgatgctc gccgaatggg
    17701 tgaatgagct gccggacggc gaaccagtat tggtggcggg tgatttcaac gactggcggc
    17761 aaaaagctaa tcatccgtta aaaatgcagg ccgggctgga tgagatattt acccgcgccc
    17821 acggacgccc ggcgcgcacg tttccggtgc aatttcctct actacgtctg gacaggatct
    17881 atgtcaaaaa tgccagcgcc agcgcgccaa ccgcgttgcc gctgcggaca tggcgacacc
    17941 tttctgatca tgccccttta agtgcggaga ttcatttatg aaatgtagct ggcgcgaagg
    18001 caataagatc cagttgctgg aaaacggcga gcaatattat cccgcgatgt ttaaggcgat
    18061 tggcgaggcg caagaacgca tcattcttga aacgtttatc tggtttgagg atgacgtcgg
    18121 caaacagctg catgcggcgc tactggcagc agcgcaacgc ggggtgaaag cggaagtctt
    18181 gctggatggc tacggttcgc cggatctcag cgatgagttt gtcaatgaac tgacggcagc
    18241 tggcgtggta tttcgctact acgatccccg tcctcgcctt tttggtatgc gcaccaatgt
    18301 gtttcgccgg atgcatcgca aaattgtggt gatcgacgcg cgtatcgcct ttattggcgg
    18361 gctgaattac tccgccgagc atatgtccag ctacggtcca gaggctaaac aggattacgc
    18421 ggtacgcctt gaagggccga ttgtcgaaga tattctccag tttgagctgg aaaacctgcc
    18481 tggacagagc gcggcacgac gctggtggcg acgtcatcac aaagcggaag agaaccgcca
    18541 gccgggagaa gcgcaggtat tgctggtctg gcgcgataac gaagaacatc gcgatgatat
    18601 tgaacgtcac tatctgaaaa tgctcactca ggcgcggcga gaagtcatta tcgccaacgc
    18661 ctacttcttc cccggctatc gatttttaca cgccttgcgt aaagcggcac ggcgcggggt
    18721 gcggatcaaa ctgatcattc agggcgaacc ggatatgccg attgtcagag tcggtgcgcg
    18781 cttgctgtat aactatctgg ttaaaggcgg cgttcaggta tttgagtacc gccgccgccc
    18841 gctccacggc aaagtggcat tgatggacga tcactgggcg acagtagggt ccagtaatct
    18901 cgatccgctc agtttgtcac tgaatctcga agcaaatgtc atcatccacg atcgtcattt
    18961 taaccagacg ctgcgcgata atctgaacgg cattattgcc gcagattgtc agcaggtcga
    19021 tgagaccatg ctgccgaaac gcacctggtg gaacctgacc aaaagcgtgc tggcgttcca
    19081 ctttttacgc cacttcccgg cgctggttgg ctggcttccg gcacatacac cacgtctggc
    19141 gcaggttgat ccccccgcac aaccgacaat ggaaacgcag gatcgggtag aaactgaaaa
    19201 cacgggggta aaaccctgat gagtaaatca cacccgcgct ggcgcttagc aaagaagatc
    19261 ctcacctggc tgttttttat cgcggtgatt gtgttgctgg tggtctacgc caaaaaagtg
    19321 gactgggaag aggtctggaa ggtcatccgc gactacaatc gcgttgcgct gctcagtgcg
    19381 gtcgggctgg tggtcgtcag ctatctgatt tacggctgct atgacctgct cgcccgtttc
    19441 tactgcggtc acaaactggc gaagcgccag gtgatgctgg tgtcgtttat ctgctacgcc
    19501 ttcaacctga cgctcagtac ctgggtcggc ggcataggta tgcgctatcg tttgtactcg
    19561 cggctagggt taccgggcag cactattacg cggattttct cgctcagtat taccaccaac
    19621 tggctgggct atattttgct ggccgggatt atctttaccg caggcgtggt ggagctgccg
    19681 gatcactggt atgtcgatca aaccacgctg cgtattctcg gcattggctt attgatgatt
    19741 atcgcggttt atttgtggtt ttgcgctttc gcgaagcacc gccatatgac catcaaaggg
    19801 caaaagctgg tgctgccttc atggaaattc gccctcgccc agatgctgat ttccagcgtt
    19861 aactggatgg tcatgggagc gataatctgg ctgttacttg gtcaaagcgt gaactatttc
    19921 tttgtactgg gcgtgttgct ggttagtagt attgccgggg tgattgtgca tattccggcg
    19981 gggattggtg tgctggaagc ggtgtttatc gcactactgg ctggggagca tacctccaaa
    20041 ggtagcatta tcgccgccct actcgcttac cgtgtgctgt attactttat cccactgctg
    20101 ctggcgctga tctgttacct gctactggaa agccaggcga agaagctgcg ggcgaaaaat
    20161 gaagcggcga tgtgaagttc ggttaagttc tttagtatgt gcatttacga ctaatgaaaa
    20221 aaccgcgtat gcctttgcca gacaagcgtt atagctatcg cggcaagtat cccaaagaag
    20281 ccgataaaat agtaatagag tgttaatgca cctgtaaaag cgcaactaac cggactccac
    20341 aactcttcgc tatggcattt tcctgccagt tcaaggagct tatttcgtcc atgcgagata
    20401 atgcctgacc ataaaacaat cattaagcaa cttattaccc aaatgggccg ttcactcatt
    20461 aatgcggcat tcctgatgat caccagggcc agtcctgcgg ttgtcaccaa acccaaagcg
    20521 gtaatgagcc atatgccgtt taatatcctg gcttcgtgct gaaaatctaa tttattgctg
    20581 ctttgcgagt atgattccat tcttttagtt atccttagaa aaaatattaa tattcaccat
    20641 caataaattt aaagccacgc atacaaaata cttttgtatt tatggatacg gaaaacactt
    20701 ccgctcggcc ataaggcata ttttatactt ggtacttgtt aagtgtcgtt ttttcgtaag
    20761 aaaaaaatcc cgacacatgg ccgggatatt gatggtgaaa aagaattaac ggcggttgcc
    20821 gaagatccgc aacaacatca ggaacaagtt gatgaagtcc agatacaagg ttaacgcgcc
    20881 aagaatggaa tatttgcgca ggttcgacgt gtcgcgggta tcaatctgct cacccatatt
    20941 tttcaatttc tgcgtgtcat acgccgtcaa tccgacaaag acaatcacgc caatgtaggt
    21001 aactgcccac atcaatgctt cgcttttcag ccagaagttg accagcgatg ccagcacaat
    21061 gccgattaac gccataaaca gcatattgcc aaagccgctt agatcgcgct tcgtggtgta
    21121 accgtacagg ctcattgcgc caaacattcc ggcagtaacg acgaaagtac tggcgataga
    21181 agcagcggta taaacaatga aaatactgga aagcgtaaga cccgtcagcg ccgaataaag
    21241 cataaagagc atcgtcgtta cacctgcgct cagcttttga atcatcgctg ataacacaat
    21301 aaccaatgct aattgcgcga tgatcagacc gattaaaaag acacggttag tgaacaacag
    21361 ctccatcaca gccgcggaat tagccgcata ccaggcaaca aatgcggtca gcaacaagcc
    21421 aacggtcatc cagccataga cttgcgccat ataagtttgc aagccagccc ggggttgtac
    21481 aattgaatca gaacgtggga atctgtccat gacgatctcc tgaagatata aggaatatct
    21541 taaggatact gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca tgtactgagc
    21601 ggtgaaattg ccggacgcag cggtgcctta tccggctaac aaaaaactac cagcgttttg
    21661 ccgcctgctg atcgctctcc cgagcttcaa cccagcggtc gccttccggc gtggcttcac
    21721 gcttccagaa cggtgcgcgg gttttgagat aatccataat aaactgcccg gcttcaaacg
    21781 cactgctgcg atgcgcactg gtgacaccga caaaaacgat ttcatctccc ggccataatt
    21841 ccccgatgcg gtgaatcacc gtgacgcgcc ccagcggcca gcggttacgc gcttcatcga
    21901 caatttctgc cagtgctttt tcagtcatcc ccggatagtg ttcgagggtt aatgctttga
    21961 cgctgtcgcc aaggttatgg ttgcgcacct taccggtaaa ggtgactacc gcaccgtctt
    22021 catcacgctc cgccagccac ggatactctt ctcctacgct gaatggctgc gggccgacaa
    22081 caattttggt ttctgccatc ttaacctccg gttaccggcg ggaagaaagc cacttcgtcg
    22141 ccgtcagtca gcggatggtc aagactcacc agcgtctggt tgacggcagc cagcaatttg
    22201 ccatcttcca gcgccagcgc ccagcgatcg ctctgcgcag ccagatgttg gcgtaacgct
    22261 tcaacggttg ggaaatccgc agccacttcg gttgcatctg ttcccaccaa ctcgcgcacc
    22321 tgggcgaaaa aaagaacttt aatcatccgc ttccacctta aagtcacccg acttgccgcc
    22381 gctcttcgcc agcaaacgta ccggaccaat taccatatct ttttgcaccg ctttgcacat
    22441 gtcataaatg gtcagcgccg ccacggaggc cgcggttaac gcttccattt cgacaccggt
    22501 tttcccggtc aggcggcata aggtttctat acgtacccga ttgtgctccg gctcggcctg
    22561 taaattgact tcaactttgc tgagcatcag cgggtgacac agcgggatca gatcccaggt
    22621 gcgttttgcc gcctgaatac cggcgatacg cgcagtggca aatacgtcgc ctttgtggtg
    22681 gcgaccatca ataatcatcg ccagcgtctc gctgcgcatg gtgacaaagg cttccgcccg
    22741 cgcttcacgc acggtttccg ctttggcgga gacatccacc atgtgtgctt cgccagcggc
    22801 gttgatatgg gtcagttgcg acatacttat ttcttcaaat gtggatggaa attgcatgga
    22861 cgtgtacggg catcgagctg cggcgcgatg atattttccc acgcggtgcg gcacgctttg
    22921 gtcgaacccg gcatggcgaa aatcagcgtt ttgttagcga cgcccgctac cgcacgagat
    22981 tgcaacgttg aggtgccaat ctcttcaaac gacaacatcc ggaacacttc accaaaacct
    23041 tcaacttcac ggtcgaacaa cggcagcagc gcttcgggag cctgatcacc ttccgtcagg
    23101 ccagtaccac ccgtaatcaa taccacttgt acatcgtcgc tggcgatcca cgcagatacc
    23161 tgagcgcgaa tagcgtagcg gttttctttc acaatggctt tatcgacaac gtgatggccc
    23221 gcttcctgcg ccgaatcgcg cagatagtga ccggaggtat cgtcttcttc accacgacga
    23281 ttagaaaccg taagaatagc aatacgggtc gggataaatt cagtgcttac ctgactcatc
    23341 tgatctctcc ttttgacgtt ttagccgcca atgtacgata agttttgcgt aataccggtg
    23401 ttgttttgat gcaggaaatg ggtctgtttc ttctcccgca gcgccgctga aatacgcgct
    23461 tccagcgcct gttgctgggt atcgtcttcc agcagatcgc gcaggttaac gccgccttca
    23521 ccaaacaggc agagatggag tttaccaatg gaggaaacgc gcaggcggtt gcaagtggcg
    23581 cagaagtctt tttcatacgg catgataagg ccaatctctc cggcgtaatc cggatgacaa
    23641 aagacttgcg cgggaccgtc gctgcgttga cgtaattggt ggatccagcc gcgacgcagt
    23701 agctcgtcac gcagaacctg accagagatg tgatgcttac ggaagagctc gctgccctca
    23761 cccgtttcca tcaattcgat gaaacgcagc tggatagggc gatgctggat ccagttcaga
    23821 aaggtgtcga gctgatgatg attaacatca cgcatcagca cggtattgac tttgaccttc
    23881 tcaaaaccgg cctcaaatgc agcatcgatc cctgccatga cctggttgaa tttatcctgc
    23941 ccggtaatgg catgaaactg gcgagcgtca aggctatcaa cgctgacgtt gatgccagta
    24001 agtcccgcat cgcgccagtt cgccacatcg cgttccagac ggtaaccatt ggtggtgacc
    24061 gcaatctggc ggatagcgtc gttttcccgc acagcggcga tgatatcggt aaagtcgcgg
    24121 cgtaaagacg gctcaccgcc tgtcaggcgc actttttcgg tgcccagact ggcaaaggcg
    24181 cgcgtaaccc ggcgaatttc atcgacggta agaaagcctt tattggtgac gccgctcggt
    24241 ttgtagccat ccggcaggca gtaggtgcaa cgaaagttac acacatcagt aatcgacagg
    24301 cgcaagtagt aaaacttacg cgcaaatgca tcagtcagtt gtgaagccat gtacaccttt
    24361 ccagatacgg gaggcgaagt catttcttcc ttcgccctgg tggcaatctt tacgagcatt
    24421 gtcacggcca aagcaccgta tcagttgacc caggtgcaga ggctagagtg tttagtggtt
    24481 aagccgatac tagcgtgtaa atgagatttt taccatccac actttcgcta tgtaatcatg
    24541 tatatagcgt catgatcgtg ctatttcgct acagaataag ggaaaatggc ggttttactt
    24601 tgatatacat catcaggaag gcatagcgta catcgtcctt tagaggtaga agaaaatagt
    24661 ttgtatcctc aatattggca ggttaattgc tgtttccccg caatttgcgc tactgtagcg
    24721 cggataatct gactccagga aactatatgc gcaatcgtac gctggctgac cttgatcgtg
    24781 tcgttgctct cggcggaggg catggactgg gacgcgttct ctcatcactt tcgtctttgg
    24841 gttctcgttt aacgggtatc gtcaccacca cggataatgg cggatcgaca gggcgtattc
    24901 gccgctcaga aggcggtatt gcctggggcg atatgcgtaa ctgcctcaac cagctgataa
    24961 ctgaacccag cgtcgcttcc gcaatgtttg aatatcgttt cggcggtaat ggcgaacttt
    25021 ccgggcacaa ccttggaaat ctgatgctga aggcactgga tcaccttagc gtgcgacctc
    25081 ttgaagcaat caatctgatt cgcaatctgc tgaaagtgga cgcacatttg attccgatgt
    25141 cggagcatcc ggtggatctg atggcgattg acgatcaggg gcatgaagtt tacggcgagg
    25201 tcaatatcga ccagttaact acgccgattc aggagttact gttaacgccg aatgtacccg
    25261 caacgcgtga ggctgttcac gctatcagtg aagcggatct catcattatt gggcctggca
    25321 gtttttacac cagcctgatg ccaattctgc tgctgaagga aatcgcacag gcattacgcc
    25381 gcacgccagc gccgatggtc tatataggca atctggggcg tgagttgagt ttacctgcgg
    25441 ctaatttgaa gctggaaagc aagctggcaa ttatggagca gtatgtcggt aaaaaggtca
    25501 ttgatgcggt catcgtcggg ccaaaagtgg atgtctcggc ggtgaaagag cggattgtga
    25561 ttcaggaggt actggaggcc agcgatattc cgtatcgtca tgaccgccag ttgttacata
    25621 gcgcgctgga aaaggcatta caggctttag gttaacggaa agccgaagga aaattccggc
    25681 ttcctattga agacaaagtg cgcgttgttt atgccggatg cggcgtgaaa gccttatccg
    25741 gcctacaaac cgcgctaatt caatgtattg cagagatcat gtaggcctga taagcgtagc
    25801 acatcaggca attttgcttt tgtcatctgt cttcttcgtt atcctgtcac gaagccgcaa
    25861 tgaacaactc acgcagctga tgcaactggt cacgaatttg tgccgcttct tcgaactcca
    25921 gattctgcgc gtgttgcatc atcaaccctt ccagttcatg gattttctgc tgcaacgctt
    25981 taggcgacat atccatcggc acattatccg gctcaacaat cggtcgcgat tttcctctgc
    26041 ccttcgcttt ggttttggca atgttctgcc ccagcgccag gatatcgacc actttcttgt
    26101 tcaagccttg cggcgtaatg ccgtgttcct cgttgtactt ctgctgtttc tcgcggcgac
    26161 gttcggtttc gccaatcgct ttcgccattg atggggtgat cttatcgccg tagagaatcg
    26221 ctttaccgtt aacgttacgt gccgcacgac caatggtctg gatcaacgaa cgttcggaac
    26281 gcaggaagcc ttctttgtca gcgtcgagga tcgccaccag cgaaacttcc ggcatatcca
    26341 gaccttcgcg cagtaagttg atccctacca atacgtcgaa ctcacccaga cgcaagtcgc
    26401 ggataatctc catacgttcg acggtgtcga tatctgagtg aagataacgc acgcgctcac
    26461 cgtgttcttc gagatattca gtgagatctt ccgccatccg cttggtcaga gttgtaacca
    26521 gtacgcgttc gttaattgcc gctcgctgac gaatctccga aagaagatca tccacctgtg
    26581 ttgccactgg ccgcacttcg ataatcgggt cgagtaagcc tgttggacgc accacctgat
    26641 ccaccacatc gccaccggat ttttccagct cgtaattacc cggcgtcgcc gaaacataga
    26701 tggtttgcgg cgctaatgct tcgaactctt caaatttcag cggacggtta tccagcgctg
    26761 atggcaggcg gaagccgtac tccaccagtg tctctttacg cgcccggtca ccgcgataca
    26821 tgccgccaat ttgtggaatg gtgacgtgag attcatcgac caccagcagc ccatcagcag
    26881 gcaggtaatc aaacagcgtc ggcggtggct cacccggtcc acgaccggag aggaagcgcg
    26941 agtagttctc aatccccgaa cagtagccta gctcgttcat catctccaga tcaaactggg
    27001 tacgctgggt cagccgctgc tcttccagca gtttgttgtt ttccaatagc actttacgtc
    27061 tggcggccag ctcttctttg atctcctcca tcgcctggac gatgcgctcg cgtggcgtga
    27121 cgtagtgcgt tttcgggtag atggtaaaac gtggaatagt ggaaacaatc tgcccggtca
    27181 gcgggtcaaa taacgacaat cgttccactt cctcgtcaaa cagctccacg cgaagtgcaa
    27241 tgtcatccga ttctgccggg aagatatcaa tcacctcgcc acgaacacgg aaagtaccac
    27301 gctggaaggc ttgatcatta cgagcatatt gcagctccgc cagtcggcgc aggatcgcgc
    27361 gctgatcgat aatcataccg accgtgagat ggagcatcat cttgagatat aaatcaggat
    27421 cgcccagacc ataaatcgcg gaaacagacg ccaccacaac cacatcacgc cgctccagca
    27481 tcgctttggt ggcggacaaa cgcatctgtt cgatatgttc gtttaccgag gcatctttct
    27541 caatgaaggt gtcggaactc ggtacatagg cctccggctg atagtagtcg tagtaggaga
    27601 cgaaatattc caccgcgttt tccgggaaga attctttcat ttcgccatac agctgggccg
    27661 ccagcgtttt gttgggcgca agtaccatgg ttgggcgctg aaggtcagca atgacattgg
    27721 caatggtgaa ggttttcccg gagccagtca cgccaagtaa cgtctggtgc gccaggccat
    27781 cttccagccc ctcttcgaga cgtcgaatcg cctccggctg atcgccagaa ggtttaaaag
    27841 cggaattcag tttgaacggt ttactcatga gtcgctacct gaaggagttg ggcgggcagg
    27901 tatgtaattt tactcgtcgt acttaattat gccaacaaat tatactggat aaaaaaacag
    27961 ttcatcacca taatatttct gttacagcgt aaactccgct gtcaatcatg agcaaaattt
    28021 actctgcggc gagataaaac tccggcctta ccgggttatc cccaaagcaa cggctttttt
    28081 aacatttgtc aagatgattg atgacagttt tatggcaaga gatgcctgtt cagtgactgc
    28141 cattgcaatt atctaacctg ttaaaaaata aaagatattt ctttgagccg tctttaacgc
    28201 caattcgcgt gcaagccgcg tattctctcg cttgcctcgt gttttctaac tctaatacac
    28261 atggttatcc acaggaatag tggataactg tctgcagccc ctatcccccg ccgcctgggc
    28321 aattcccaca ttcaccgcat gatgcggagg taaaattttt ttcgtgattt tttta
//
LOCUS       DAZKJR010000060        28222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKJR010000060 DAZKJR010000000
VERSION     DAZKJR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.060"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            275..946
                     /locus_tag="ACHOWS_002578"
     CDS             275..946
                     /locus_tag="ACHOWS_002578"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFQ0048131.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDIEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            956..2152
                     /gene="yaiP"
                     /locus_tag="ACHOWS_002579"
     CDS             956..2152
                     /gene="yaiP"
                     /locus_tag="ACHOWS_002579"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0048132.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2187..2711
                     /locus_tag="ACHOWS_002580"
     CDS             2187..2711
                     /locus_tag="ACHOWS_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0048133.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2713..3486
                     /gene="yaiO"
                     /locus_tag="ACHOWS_002581"
     CDS             2713..3486
                     /gene="yaiO"
                     /locus_tag="ACHOWS_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFQ0048134.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3671..3946
                     /gene="frmR"
                     /locus_tag="ACHOWS_002582"
     CDS             3671..3946
                     /gene="frmR"
                     /locus_tag="ACHOWS_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFQ0048135.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3981..5090
                     /gene="frmA"
                     /locus_tag="ACHOWS_002583"
     CDS             3981..5090
                     /gene="frmA"
                     /locus_tag="ACHOWS_002583"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFQ0048136.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5182..6015
                     /gene="fghA"
                     /locus_tag="ACHOWS_002584"
     CDS             5182..6015
                     /gene="fghA"
                     /locus_tag="ACHOWS_002584"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFQ0048137.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFAPIVSPSQVPWGQQAFTAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6242..6781)
                     /gene="yaiL"
                     /locus_tag="ACHOWS_002585"
     CDS             complement(6242..6781)
                     /gene="yaiL"
                     /locus_tag="ACHOWS_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFQ0048138.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDYLMW"
     gene            complement(6883..8094)
                     /gene="mhpT"
                     /locus_tag="ACHOWS_002586"
     CDS             complement(6883..8094)
                     /gene="mhpT"
                     /locus_tag="ACHOWS_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFQ0048139.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGIGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8270..9283)
                     /gene="mhpE"
                     /locus_tag="ACHOWS_002587"
     CDS             complement(8270..9283)
                     /gene="mhpE"
                     /locus_tag="ACHOWS_002587"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFQ0048140.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9280..10230)
                     /gene="mhpF"
                     /locus_tag="ACHOWS_002588"
     CDS             complement(9280..10230)
                     /gene="mhpF"
                     /locus_tag="ACHOWS_002588"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFQ0048141.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10227..11036)
                     /gene="mhpD"
                     /locus_tag="ACHOWS_002589"
     CDS             complement(10227..11036)
                     /gene="mhpD"
                     /locus_tag="ACHOWS_002589"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFQ0048142.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11046..11912)
                     /gene="mhpC"
                     /locus_tag="ACHOWS_002590"
     CDS             complement(11046..11912)
                     /gene="mhpC"
                     /locus_tag="ACHOWS_002590"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFQ0048143.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11930..12874)
                     /gene="mhpB"
                     /locus_tag="ACHOWS_002591"
     CDS             complement(11930..12874)
                     /gene="mhpB"
                     /locus_tag="ACHOWS_002591"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFQ0048144.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12876..14540)
                     /gene="mhpA"
                     /locus_tag="ACHOWS_002592"
     CDS             complement(12876..14540)
                     /gene="mhpA"
                     /locus_tag="ACHOWS_002592"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFQ0048145.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14731..15564
                     /gene="mhpR"
                     /locus_tag="ACHOWS_002593"
     CDS             14731..15564
                     /gene="mhpR"
                     /locus_tag="ACHOWS_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFQ0048146.1"
                     /translation="MQNNEQTAYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15641..16723
                     /gene="lacI"
                     /locus_tag="ACHOWS_002594"
     CDS             15641..16723
                     /gene="lacI"
                     /locus_tag="ACHOWS_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFQ0048147.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            16846..19920
                     /gene="lacZ"
                     /locus_tag="ACHOWS_002595"
     CDS             16846..19920
                     /gene="lacZ"
                     /locus_tag="ACHOWS_002595"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFQ0048148.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSKEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDISLLHK
                     PTTQISDFHVTTRFNDDFSRAVLEAEVQVYGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVLRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLTENLSVTLPSAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQTEVALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19972..21225
                     /gene="lacY"
                     /locus_tag="ACHOWS_002596"
     CDS             19972..21225
                     /gene="lacY"
                     /locus_tag="ACHOWS_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFQ0048149.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21291..21902
                     /gene="lacA"
                     /locus_tag="ACHOWS_002597"
     CDS             21291..21902
                     /gene="lacA"
                     /locus_tag="ACHOWS_002597"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFQ0048150.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVHFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVESSV"
     gene            complement(22005..23186)
                     /gene="cynX"
                     /locus_tag="ACHOWS_002598"
     CDS             complement(22005..23186)
                     /gene="cynX"
                     /locus_tag="ACHOWS_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFQ0048151.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQNAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23192..23662)
                     /gene="cynS"
                     /locus_tag="ACHOWS_002599"
     CDS             complement(23192..23662)
                     /gene="cynS"
                     /locus_tag="ACHOWS_002599"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFQ0048152.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23693..24352)
                     /gene="cynT"
                     /locus_tag="ACHOWS_002600"
     CDS             complement(23693..24352)
                     /gene="cynT"
                     /locus_tag="ACHOWS_002600"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFQ0048153.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSITAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24462..25361
                     /gene="cynR"
                     /locus_tag="ACHOWS_002601"
     CDS             24462..25361
                     /gene="cynR"
                     /locus_tag="ACHOWS_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFQ0048154.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTTVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(25363..25454)
                     /locus_tag="ACHOWS_002602"
                     /pseudo
     CDS             complement(25363..25454)
                     /locus_tag="ACHOWS_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25494..26777)
                     /gene="codA"
                     /locus_tag="ACHOWS_002603"
     CDS             complement(25494..26777)
                     /gene="codA"
                     /locus_tag="ACHOWS_002603"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFQ0048155.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26767..28026)
                     /gene="codB"
                     /locus_tag="ACHOWS_002604"
     CDS             complement(26767..28026)
                     /gene="codB"
                     /locus_tag="ACHOWS_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFQ0048156.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         6757 a         7668 c         7314 g         6483 t
ORIGIN      
        1 agaacatatt tttaatatgc aattaattga caaactaaat cacatttaaa tacaatatat
       61 aaaacaaaat aaccatacaa accaaacaca caaacactta acatatgaac tacataaaga
      121 ttatacagtc ttatccccgt acctggcagc cccttgtttt tcagctatca gctattttta
      181 acacttaaaa attagcatct gtctgcgtca gaggttacgt ctctgcgggc gtcgttgtgc
      241 ttgtagtata ataaataaaa tggagtgtca taaagtggat aaggttttag attcagcgat
      301 cctttcttcg gcaaataaaa gaaagggtat ccttgccatt ggcgcgcatc ctgacgatat
      361 agaattaggc tgtggcgcat cgctagctcg ccttgcgcaa aaaggaattt atattgctac
      421 cgtggtgatg actaccggta attctggcgt agatggaata atcgatcgcc acgaagaatc
      481 acgcaacgct ctaaagatat tagggtgcca ccaaactatt catcttaatt ttgctgacac
      541 ccgcgctcat ttacagctca atgatatgat ttctgccctg gaaaatatca taaaaaatca
      601 aattccctct gatattgaaa ttatacgtgt atataccatg catgatgctg accgccatca
      661 ggatcatctt gcagtctatc aagcctcaat ggttgcctgt cgcgctattc cgcaaattct
      721 cggctatgaa acaccaagta cctggctttc atttatgcct caggtttttg aatccgtgaa
      781 agaagaatat ttttctctta aactgactgc gttaaaaaaa cataaaagtc agagccagcg
      841 agattatatg cgcccggagc gtctgcgtgc agttgctcaa tttcgcgggc aacaggtcaa
      901 tagcgatctg ggtgaaggtt ttgttattca taaaatgatt ctttgagcaa attgcatgaa
      961 aacctggatc tttatatgta tggccgtagc aatattgcta tggtttctga gtacgttaag
     1021 gcgtaagccc agccaaaaga aaggctgtat tgacgccatt atacctgctt ataacgaagg
     1081 cccgtgtctg gcgcagtcac tggataatct gctgcgtaac ccttatttct gccgggtaat
     1141 ttgcgttaac gacggctcca cggacaatac cgaagcggtc atggcggaag tcaaacgcaa
     1201 atggggcgac cgctttattg ccgtcacgca aaaaaatacc ggtaaaggtg gtgcgctaat
     1261 gaatggcctc aattacgcca cctgcgatca ggttttttta agtgatgccg acacctatgt
     1321 tccgcccgat caagacggaa tgggctatat gctggcagaa atagagcgcg gtgctgatgc
     1381 cgtaggcggc attccctcta ctgcgttgaa aggcgcgggt ctgttaccgc acatccgcgc
     1441 gaccgtaaag ttgccgatga ttgttatgaa gcgcacgcta cagcagctcc tgggcggcgc
     1501 accgtttatt atcagcggtg cctgcgggat gttccgtact gatgtattgc gtaagttcgg
     1561 tttctctgat cgtactaaag tcgaagacct tgatctcacc tggacattgg tggcaaacgg
     1621 ctatcgtatt cggcaggcga atcgctgcat cgtataccca caggaatgca acagcccgcg
     1681 tgaggaatgg cgtcgctggc ggcgttggat tgtgggctac gcggtctgta tgcgcctgca
     1741 taaaagactt ttatttagcc gcttcggtat cttcagtata tttcctatgc tgttggttgt
     1801 gctttatggc gttgggattt atctcactac ctggtttaat gaattcatca ccaccgggcc
     1861 acatggtgtg gtgttggcaa tgttcccgct tatctgggtc ggcgtagttt gtgttattgg
     1921 tgcatttagc gcctggtttc atcgttgctg gttgttggtg cctttagcac cgctttccgt
     1981 tgtgtatgta ttattagctt atgccatctg gattatttat ggacttattg ccttttttac
     2041 tggacgcgaa cctcagcgcg acaaacccac ccgctattcc gcactggtgg aagcgtcaac
     2101 cgcttattcc cacccttctg tcacaggaac tgaaaaacta tctgaagctt aatgttaaag
     2161 agaaaaatat tcagattgca gaccaggtga ttattgatga aagtgccggt gaggttgtta
     2221 tcggcgcgaa tacccgtatt tgccacggtg ccgttattca gggtccggta gtgattggcg
     2281 caaactgcct gataggtaat tatgcgttta ttcgtcctgg cacaataatc agcaatggcg
     2341 taaaaattgg ttttgccacc gaaattaaaa atgcggttat tgaagcggaa gcaacgattg
     2401 gtccgcaatg ctttattgcc gactcggtag ttgcaaacca ggcatatttg ggcgcacaag
     2461 tacgaaccag taatcatcgt ctggatgaac agcccgtgtc tgttcgaact ccagagggaa
     2521 ttatcgctac cggatgcgat aaattaggtt gttatatcgg gcagcgttca cgccttggcg
     2581 tacaagttat tattttgcct gggcgaatta tttccccgaa cacgcaactt ggcccgcgcg
     2641 tgattgtaga acgtaattta cctaccggaa cttattcact ccgacaagaa cttatccgta
     2701 caggagatta aaatgataaa acggacgtta ttagcggcgg caatatttag cacattgccc
     2761 gcttatgccg ggttaacttc cattaccgcg ggctacgatt ttaccgatta ttctggcgat
     2821 catggcaacc gtaatttagc gtatgctgaa ctggtggcga aagttgaaaa cgcaacgctg
     2881 ctttttaatc tttcccaggg gcgtcgtgat tatgaaactg aacatttcaa tgccactcgc
     2941 ggtcagggtg cggtctggta taaatggaat aactggctga caacccgaac gggtattgcc
     3001 tttgcggata atacgccggt ctttgcccgc caggattttc gtcaggatat taacctggcc
     3061 ctgttgccaa aaacgctttt cacgaccggt tatcgctaca ctaaatatta cgatgatgtc
     3121 gaagtcgatg cctggcaagg cggcgtatcc ctctatacag gcccggtgat caccagctat
     3181 cgctataccc attatgactc cagcgatgca ggtggtagtt acagcaatat gatttccgtg
     3241 cgtctgaatg acccgcgcgg cactggttat acgcaactat ggctaagccg cggaacaggt
     3301 gcttacacct atgactggac gccagaaaca cgctacggca gcatgaagag cgtcagtttg
     3361 caacgtattc aaccgctgac tgagcaactt aatctcgggc tgacggcagg taaagtgtgg
     3421 tacgacaccc caaccgatga ttataacggt ctgcaacttg cagcccatct gacctggaaa
     3481 ttctgattcc ttctgccgcc cgctatccgg ggcggccttc cctgtcgatt aggcccctct
     3541 ttactttttg ttatccgact acattcaccg gataaatttt attctccagt gttatatact
     3601 atagaggggt atgcattgac atataacata cccccctata gtatattgcg tgcagatgat
     3661 gaggtgcgaa atgcccagta ctccggaaga gaagaaaaag gtccttactc gagttcgtcg
     3721 tattcggggg cagattgatg ctctggaacg gtcgctggag ggtgatgccg aatgccgtgc
     3781 catactccaa cagatcgctg ccgttcgggg cgcggctaac ggactgatgg cagaagtgct
     3841 tgaaagccat atccgggaaa cgtttgaccg aaatgactgc tacagccgcg aagtcagcca
     3901 atccgttgac gacactattg aactggttcg tgcctatctt aaatagctga atctattacc
     3961 atattgagga agagcgagag atgaaatcac gtgctgccgt tgcatttgct cccggtaaac
     4021 cgctggaaat cgttgaaatt gacgttgcac caccgaaaaa aggtgaagtg ctgattaaag
     4081 tcacccatac cggtgtttgc cataccgacg catttaccct ctccggcgat gacccggaag
     4141 gtgtcttccc ggtggttctc ggtcacgaag gggccggggt tgtagttgaa gtcggtgaag
     4201 gcgtaaccag cgtcaaacct ggcgaccatg tgatcccgct ttacaccgca gagtgcggcg
     4261 agtgtgagtt ctgtcgttct ggcaaaacca acctgtgtgt cgcggttcgc gaaacgcagg
     4321 gcaaaggctt gatgccagac ggcaccaccc gtttttctta caacgggcag ccgctttatc
     4381 actacatggg atgctcaaca ttcagtgaat acaccgtggt cgcggaagtg tctctggcca
     4441 aaattaatcc agaagcaaac catgaacacg tctgcctgct gggctgtggc gtgaccaccg
     4501 gtattggcgc ggtacacaac acagctaaag tccagccagg tgattctgtt gccgtgtttg
     4561 gtcttggcgc gattggtctg gcagtggttc agggcgcgcg tcaggcgaaa gcgggacgga
     4621 ttatcgctat cgataccaac ccgaagaaat tcgatctggc gcgtcgcttc ggtgccaccg
     4681 actgcattaa cccgaatgac tacgacaaac cgattaaaga tgtcctgctg gatatcaaca
     4741 aatggggtat cgaccatacc tttgaatgca tcggtaacgt caacgtgatg cgtgcggcgc
     4801 tggaaagtgc gcaccgcggc tggggtcagt cggtgatcat cggggtagct ggttctggtc
     4861 aggaaatctc cacccgtcca ttccagttgg tcaccggtcg cgtatggaaa ggttccgcgt
     4921 ttggcggcgt gaaaggtcgt tcccagttac cgggtatggt tgaagatgcg atgaaaggcg
     4981 atatcgatct ggaaccgttt gtcacgcata ccatgagcct ggatgaaatt aatgacgcct
     5041 ttgacctgat gcatgaaggc aaatccattc gaaccgtaat tcgttactga tttcccgcag
     5101 gtttacccgt ccagttcaga cggggttctt aatactctcc ccgggcagcc gtccggggga
     5161 ttaaccgtga gataatgact gatggaactc attgaaaaac atgccagctt tggcggctgg
     5221 caaaatgtgt atcggcatta ttccccatca ctgaaatgtg aaatgaatgt cggcgtctat
     5281 ctcccgccaa aagccgcaaa tgaaaaattg ccggtgttgt actggctttc aggcctgacc
     5341 tgcaacgagc agaatttcat tactaaatcg gggatgcagc gttacgcggc tgagcacaac
     5401 attattgttg ttgcgccaga caccagtccg cgaggcagtc atgtcgcaga tgccgaccgt
     5461 tacgatctcg ggcaaggtgc cgggttttac ctgaacgcaa cgcaagcgcc gtggaatgaa
     5521 cattacaaaa tgtatgacta tatccgcaac gagctgccgg atttagtgat gcaacatttt
     5581 ccggcaacga ccagaaagtc tatctcaggt cattctatgg gcgggctggg cgcgctggtg
     5641 ctggcgttac gtaacccaga tgaatatgtc agcgtctcgg cgtttgcgcc cattgtctcc
     5701 ccatcgcaag taccgtgggg acagcaagcc tttaccgcat atcttggtga aaataaagat
     5761 gcctggttgg attacgatcc ggtgagtctt atttcacaag gtcaacgcgt tgcggaaata
     5821 atggttgatc aggggttgag tgatgatttt tacgcagaac agctgcggac tccaaatctt
     5881 gaaaagatct gccaggagat gaatatcaaa acgttaatcc gttatcacga gggttatgat
     5941 cacagctatt attttgtctc cagttttatt ggcgagcata ttgcgtacca cgccaataaa
     6001 ctgaatatgc gttgagcatc cgcgcacgca tgccggatgc ggcgtaaacg ccttatccgg
     6061 cctacatttc acccgtaagc cgtaggcctg ataagacgcg aaaagcgtcg catcaggcat
     6121 ctgcgcacgg ctgccggatg cggcgtgaac gccttatccg gcctacactt cgcccgtaaa
     6181 ccgtaggcct gataagacgc gtaagcgtcg catcaggcat cactgcacgg cttatctatg
     6241 tttaccacat caaataatca ggcactttga agtcggcata cggatcgtct tcatcctgct
     6301 cttcggcgct gagcgcgctg tgcaacacaa tactgctggc atcacgctga gcaattttat
     6361 cggcaacact ggcgggaata atggcgtatt caccttcgct attgttatcg accaacaagc
     6421 gggcaatcgc cagacgacca ttaatcaact gcgcctgtgt gagcttatcg acaaaaatct
     6481 tcttaatcag attgccgtca gtgaagttaa aaccaatatc gccattggca atggtgattc
     6541 ggttcatttc aatgagctgc ttcacctggg ctttatattc tttcgccaac gccgcttgtt
     6601 tttgctgttc gctaagctgt ttatcacgct caagctgtgc ctttttattt tcttctaccg
     6661 ccgcccgagc ttcacgcgcc tgaacgcgcg attttttcgc cgttctctcg accttcgccg
     6721 cttttttgct ggtgactaat cctgctttga gcaactgctc ttgtaaggta agttttgcca
     6781 tctttgtttc caaacccgct gaataatttt tagcgagtat acctgtaaac gccgtaggtg
     6841 tattgggggc aagtcccttt cgcccctgct taataaggcg cgtcaggcat cggcgcacgg
     6901 ctgcattcgt gatctccggc tcatcaaaat aaatactgcc aggccagcaa caagaatacc
     6961 cggcgcagaa gcggccatta cgccaaccgt gccaatgcct aatgccagca ttttcccggc
     7021 cagtaacgga ccgctcatag cccccagacg ccctaccgcc acggctgttc ccacacctgt
     7081 tgcgcggatc tgcgaactgt aaaacaacgg tgccagggca tacaaaacgc tttgcccacc
     7141 tgtcgcaaac aaccccgcga cgaatcccgc cagcaacata ccgttaaatg acgacaccgt
     7201 tcccagcgcc agcagcgaag ctaacatgcc gctataaatc agtagcgaca tggttactgg
     7261 acgcagctta tccatcaatg cgcccaacat taacgtcccg cttgccgccc ccatttgcag
     7321 tgcaaacatc acccctgccg cctgcgatgg ctggaatcct tgctccacca aaagcagcgg
     7381 tagccagttg atcaacatgt agaccaccag cagagtgaag aaataacaca accacagcag
     7441 cagcgtcgcg gttgccgttt ctggcgcaaa taacgcacgc agtggtggcg cagcctgttt
     7501 ttcgccagcg aacaccgccg actccggcag ccagcgcatt aatagcggca ccagaatcaa
     7561 cggcaccaca ccacctaccc aaaacaccgt ttgccatgct aagtttgccc ccgcgaaacc
     7621 cagtgtcgcc gccagcgccg cgccaatggg gacaccgcaa tacatcaggc tcactgccgt
     7681 cccacgaaaa cgtggacccg cggcttcaga cgtcagggcg atcagattcg gcaacgccgc
     7741 ccccagcccg acaccggtca tcagccgcgc aaagaccagt gaggggaaat cccaggcaat
     7801 cgccgttgcc agtgagaaca aaccaaacag cgcaactgag ccaatcaaaa tgcgcttgcg
     7861 accataacgg tccgccagca ttccgccaac caacgcgccg ggtagcaaac cgagtattcc
     7921 ggcgctgaat atccagccca tttgcatttt atcgagtgcg aaagcctggg cgatgccacc
     7981 cgccgcaatg ccagccgcct gaagatccag tccttccatc agagcgacca aaaaacaaag
     8041 cccgatggtc agcatcaggc gggttgaaga tgatgaaggg gtacgagtcg acataagatg
     8101 aacctcgttg cgagtgcagg tgaatcgcgg ctgatacatt gtcagccgcg taatcagaac
     8161 catttaatga ctgcctacgg gcgaaatata ggccggataa ggcgtttacg ccgcatccgg
     8221 cagtcgtgct gggatgggca tacaaaatgc acgttctcgg cagtcatcat tatttgttgt
     8281 tgcgcagatc cagcgccacg tcgacgatca tatcctcctg gccgccaacc atccggcgtt
     8341 tgcccagctc aacgagaata tccaccgcgc ttaagccata acgcgccgcc gccgtttcac
     8401 agtgacgcag gaagctcgag taaacaccag cgtatcccag cgccagcgtt tcgcgatcga
     8461 ctcgtaccgg tcgatcctgc aacggacgca ccaggtcgtc agcggcatcc attaacgcat
     8521 agagatcggt cccatgctgc caacccagtt tatccgcggc ggcaataaaa acttccagcg
     8581 gcgcgttacc tgcgcccgcg cccattcccg cgaggctggc gtcgattcgg tcgcagccct
     8641 cttccaccgc cgcgatagag ttcgccacgc caagactcag gttatggtga gcgtgtatgc
     8701 cggtttgcgt ttctggtttc agcactgctt tcagagcgcg gaaacggtca cggatatcgc
     8761 tcatgttcat cgcaccgcca gaatccacca cataaataca ggtcgcaccg tagccttcca
     8821 tcagctttgc ctgctttgcg agattctccg gcgtggtcat atggctcatc atcagaaaac
     8881 caacggtgtc cattccgagc tcgcgggcat actgaatatg ctgggcggaa acatcagctt
     8941 cggtacagtg cgtagccaca cgaaccaccc gcgcgccagc ctgccaggca tttttcagat
     9001 cgtgaatagt gccgattcca ggcagcaaca acgtcgcgat tttggcgtgc ttcaccacat
     9061 ccgccgccgc ttcaatccat tcaaggtcgc tatgtgcgcc gaaaccatag ttaaagctgg
     9121 aaccttgcaa accgtcgccg tgggccactt caatcgaatc cacgcgggca tcgtccagtg
     9181 ctttggcaac ctggcgaacg ttttccaatg aatactgatg acgaatggcg tgcataccgt
     9241 cacgcaacgt gacgtccgag atataaagtt ttttaccgtt catgccgctt ctcctgcctt
     9301 gcgcgccagt gactgggcca ttttttccgc tgtcgccagc gcactggaag tcataatgtc
     9361 gaggttgcct gcataggcag gcagataatg cgctgcgcct tcgacttcca gccagaccgc
     9421 cgttttcagt ccggagaact gccccacgcc cggcaaatta accggtttat cctgcgggat
     9481 aacttcaaac tgcacgcgct gtttcaggcg ataacccggt acgtaagcct gcaccgcctc
     9541 agccatttca ttgattgagg cttcgatatc atcttgtgaa gcttcgtcgc tcaatacata
     9601 caccgtgtca cgcatcatca acggtggctc tgctgggtta agcacaataa tcgccttccc
     9661 ttttgccgcg ccacccacca cttcaatggc gcgcgaagtg gtttccgtaa attcatcgat
     9721 attggcacgc gtgccaggtc cggcagattt actggcaata gaagcgataa tttcggcgta
     9781 atgaacacgc gccacgcgtg aaactgccgc caccattgga atggtggcct ggccgccgca
     9841 ggtgaccatg ttgacgttca gttgagcgac gttctcctcg aggttaacga ccggcacgca
     9901 gtaggggccg atggcagcag gcgtcaggtc aattaagcga atatccggtt tcgcttcgcg
     9961 caaagcggca tcgtttttca catgcgcacc cgcgctggtc gcatcaaata caatgtcgat
    10021 atcagcaaat tcaggcatgt tcatcaggcc gatcacccct tcatgggtgg tggcgacgcc
    10081 catacgtctg gcacgcgcca gaccgtcgga ctgaggatca atgccgacca tcaccgccat
    10141 ctccagatgc tggccgtgac gcaaaatttt aatcatcaga tcggtgccaa tgttgcctga
    10201 accgataatg gcgactttac gcttactcat gacagacttc cttttggggc tgcgcttgaa
    10261 aatgtcgcag caactgaacc tatgccttca atatgggctt caaaacgatc gcccgcattc
    10321 accgccacca tcggacctaa tgccccggta agaatgatat ctccggcgcg cagcggttca
    10381 cccaggctgg ccattttgcg tgccagccag acggcggcat taagcggatg tcccaggcat
    10441 tcgctgccgc gcccgctgga aacctcttcg ttattacgcg tcatcttcat ggcgcagttt
    10501 ttcaggtcta accccgccgg acgttgcgcc ggaccgccga tgacatacac cccgcaggaa
    10561 gcgttatctg ccacggtatc gacaaactga atcgaccagt cgcgaatgcg gctccccacc
    10621 acttccagcg ccggaagtac ccattcaatg gcgttataca attcgtcgaa ggtgatatcg
    10681 gttgcgggta gatcgcggtt caacaccagt gcaatctccg cttcaatgcg gggttgcaga
    10741 acacgggaaa aaggaatggt ttcgttatcg ccataacaca tgtcggcaaa taatgtcccg
    10801 aaatccggtt gatcaacgcc cagttgttgc tgcactttcg gatgcgtcag ccccactttg
    10861 cgccccacca ctcgccgtcc ctgagcgacg tcgtgttgca cattgatgtg ctgaatggcg
    10921 taggccgctt cagcgttatc gataccaatc agatcgcgca gcggcgcaat cgcttcgccc
    10981 tgctctgcgg cgcgacgtaa atccgccgcc agttgctcaa gagtatgctt cgtcatgacc
    11041 gttccttaag ggcgtgcgag gaaattcagc accagttgat tgaaagcgtc ggcatgttcc
    11101 cactgcgccc agtgaccaca gtcgcggaag atatgcagtt cagaaccggc aatgccggac
    11161 agcagacgca gacccgcatc catcggcaca aagcggtcgt tgcgccccca gacaatcagg
    11221 gtttgcgctt tgatttccgc cagacgtggg ccaaaatccg ggaactgttt cggattggct
    11281 tccaggctct taacgaagtt ttccaggtga tcgcggcgcg acagcatatt attcaggcgc
    11341 gcttcaaaca gggcgtcggt caaatcgctg gtatcaaaaa cgaagatatc catcatcagc
    11401 ttcaggtttt cgatagtcgg ctgacgataa agctgattca gtcgcttaat accttcggtt
    11461 ggcatcggcg taaacaaact catgccgccc gtaccgccgc ccatcagcac cagtttgccg
    11521 acgcgctccg gccagttaag ggtgaacgcc acagaactgt ggccgcccat cgagttgccc
    11581 agcaggtgga ttttggcgat atccagttga tccaccacgc ttttcaggat tcgcgcatta
    11641 agatccgatc gtgaaccact attaacgatc gaatcgctct taccccaacc cggacaatcc
    11701 agcaggatca cccgatagcc cgcctttacc agcggatcga tattgcggct gaagttcgcc
    11761 cagccagtag cacccgggcc ggaaccatgc agcaggacaa cagtttcgtc gccttgtccg
    11821 cagtcattaa aatggatgcg cagcgtttta cccgcttctt ctacattcag aaaacggctg
    11881 gtggcggctt cggtttgtgg ctgataactc atcatcttct cctgcatatt cagttctctg
    11941 ttctggcgct taacgagcca aatccggcaa tccactccgg gattgggcgg taataacgcc
    12001 cttcgctacg ccagttgcca aacgcagaaa tagcggcaaa agcggcgacc caggttttga
    12061 tttcatgtgt cgactttccg gcaatggcgg aaagctcttc gttactgacg gcatccagtt
    12121 cctgtatgcg tccctgctcc agcaaagtca tgaactggtt atcccaaatc gggttgagcg
    12181 gatgcagcgt tctctgatcc tcaacaaact tctcagcggc gctaatcacc cgttgctgac
    12241 gcaattcgcg ctcactggcg ggtaaatctt tcccgctccc caacagacgg tcgcgcatat
    12301 gggcatcggc tttcgccagt tctggcaccg gcggctgatg ggaaagccca ccggaaccca
    12361 ggaacagcac gcgtttattg agagtactgg tgaaacgtcc aatggcttcc cccaacatgc
    12421 gggtacgctg gaaaccgggc aacggcgtgg cgacaccgtt gatgaacaca ggcagaaccg
    12481 gcaccttatc cagcccaccg agcaggaact ccagcggctg ggcgaacccg tggtccacct
    12541 gcatacagta agaaacggca agatcgattc cgctcttcat gacggcatgc gcacaggcct
    12601 ccgccagctc aacaggcacg ggcagctctc ctgccgcact accgaaatca ccaattgccg
    12661 tcgctccaac gcctaaacag aacggtggca tcacgtcata gaaaaagccg ttgtagtgat
    12721 ctggcgcaaa cagcaccacc aactcagggg agaaagccgc aatacgctcg cgggcgctgg
    12781 caatcacgcc attgacctca tcgagcacct cttgcgccgg gtcgacatat cccaccagcg
    12841 gcgagtggga aagacagtga agataagcgt gcatatcagg ctaccttttc gacagaaacg
    12901 tcggcatcag ggcgggtcag cgtcattacc gacgccagtt tattcagcgt attacccaga
    12961 gtttgcggaa tggcggtggc ggcaacaaag cgatccgggc gcatcaccac cagcgaagca
    13021 ttgtgttgcg cgaaccagct acgcaggcga ccttgcgtat cgcccacgcg tagtacgccg
    13081 tcgtggttat cctgtgcggt atgaatttgc acttccggca ccacctgaat gaagcgtgtg
    13141 cccaacgcgc gccactgctg gatttgctcg tcgctcatcc cccacagtgg attgcatccc
    13201 cagccaatta ccgcgaagtt cgcgccgatc gcgttatcga gcagcgtcac gtcgccgttt
    13261 tccagcgtga ctttcggctg aataaacatc ttgccgaccg gagagtgctt cgcttcgccc
    13321 tcacgcacca gcgcaccgcc gtaatattgc ggcatcggct tgaagcgcat ttcgaggaag
    13381 tagcgtttta ctggcggcag ataattcaac agccaggaaa cgccgtcacg taacgtaccc
    13441 tgccagcgtt tcggcggagc cagcacgttg cccgccgtca cggacaggtc aatcatcgct
    13501 ttggcgtgat cgcgacgttc ttgttgatag gtatcgagca gcgcatcgcg ggctttcccc
    13561 tggataacca acgccagttt ccatgcgagg ttaaaggcgt cgcgcatacc actgttatag
    13621 ccctgcccct gccacaccgg catgatgtgc gcggcatcgc ccgccagcag tacgcgatca
    13681 atacgaaaac gttgcgccag tcgcgcgttg tgggtgtaga cacgctggcg aatcaattca
    13741 acattgtccg gattaggcag cactttgctt aacagcttgc gcatattttg cggctcacgc
    13801 agctgttctt cggtttctcc cggcatcacc ataaattcaa agcgacgtac cgcatgaggc
    13861 agcgcggcag aaacatacgg gcgcaccgga tcacaacaca aatagatatg cggcgtactt
    13921 aacggatcgt tggcgatatc taccacaatc cactgatttg gcgcagtttt accttcaaac
    13981 ggcacattca gggtgcgacg gacaaaactt gccccaccat cacaggccac cagccattgg
    14041 gctttgaccg tttcccgctg cccttctgcc gttttcaggt gcaaggtcac ttcgtcatct
    14101 tgctggctga aggcctccag ctcgcgggaa aacaggcagc gcacattcgg aaaacgcgac
    14161 aatccttcca gcatcaccgc atcgacctga ggctgaataa aggcgttacg gcgcggccag
    14221 ccaaattcat cggtcattgg ctgaatatca gcaaaacagc gaccttttgg ggtgagaaaa
    14281 cgcatcgcgt gccacggcgt agtgtgcggc agaacattat cgaccaggcc gaccgactgc
    14341 atggtgcgca gcgcctcgtc atcaatacca atcgcacgcg ggtagtcgat caacttatcg
    14401 agtttctcca ccaccagcac gtcaatgccc atctggccga gatagttcgc catcatcagc
    14461 ccgaccggac cggcaccagc gatcgccacc tgaacgctat ggttaacagc aggctggatg
    14521 tcaggatgtt gtattgccat ttcagtacct cacgactcgg acaaaatgcc gttgcgcgca
    14581 cagtacggcg caacttattt tgttaaaaac atgtaaatga ttttttattg tgcgctcagt
    14641 ataggaaggg tgttttcggc tacaatcaaa acatgcccga atgtgcacca agtgcacaac
    14701 gttgttttaa ctatagaaat gtcaattaat atgcagaaca atgagcagac ggcatacaaa
    14761 accgtgcgcg gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc
    14821 ggtgccagcg tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga
    14881 ctgctggaga cgctgcagga agagggatat gtccggcgta gtccctccga tgatagtttc
    14941 cgtttgacca tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct
    15001 gcactggcgg ccccactgct gggcgatctg ttgcgcgaag tggtatggcc gacagatgtg
    15061 tccaccctgg atgttgatgc aatggtggta cgcgaaacta cccatcgttt cagccgctta
    15121 tcttttcacc gggcaatggt cgggcgacgg ttaccgcttc tgaaaaccgc ctcgggcctg
    15181 acctggctgg ccttttgccc ggaacaagaa cgcaaggaat taatcgagat gttagcctcc
    15241 cgccccggtg atgactatca actggcacgg gaaccgttaa agctggaagc cattctggcg
    15301 cgcgcgcgca aagaggggta cggacagaac taccgcggct gggatcaaga agaaaagatc
    15361 gcctctattg ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg
    15421 tatatggcga gcgcaatgac cattgaacag gcagcggaaa agcatcttcc ggcgctacaa
    15481 cgggtggcaa aacagatcga agaaggggtt gaatcgcagg ctattctggt ggccggacgg
    15541 cgaagcggcg tgcatttacg ttgacaccat cgaatggcgc aaaacctttc gtggtatggc
    15601 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgctata
    15661 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
    15721 ctgccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
    15781 cattcccaac cgcgtggcac aacaactggc gggcaagcag tcgttgctga ttggcgttgc
    15841 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
    15901 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
    15961 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
    16021 gctggatgac caggatgcca ttgctgtgga agctgcctgc gctaatgttc cggctttatt
    16081 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
    16141 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcagg
    16201 tccattaagt tctgtctcgg cgcgtctgcg tctggcgggc tggcataaat atctcacacg
    16261 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
    16321 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
    16381 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
    16441 tatctcggta gtgggatacg acgataccga agacagctcg tgttatatcc cgccgttaac
    16501 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
    16561 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
    16621 aaccaccctt ccgcccaata cgcaaaccgc ttctccccag gcgttggcag attctttaat
    16681 gcagctggcg cgacaaattt cccgactcga aagcgggcag tgagcgcaac gcaattaatg
    16741 tgagtcagct cactcattag gcaccccagg ctttacactt tatgcgtccg gctcgtatgt
    16801 tgtgtggaat tgtgagcgga taacaatttc acacaggata cagctatgac tatgattacg
    16861 gattcactgg ccgtcgtatt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    16921 aatcgccttg cggcacatcc ccctttcgcc agctggcgta atagcaaaga ggcccgcacc
    16981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
    17041 gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgacgc cgatactgtc
    17101 gtcgtcccct caaactggca gatgcacggt tacgacgcgc ccatctacac caacgtgacc
    17161 tatcccatta cggtcaatcc gccgtttgtt cccgcggaga atccgacagg ttgttactcg
    17221 ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
    17281 ggcgttaatt cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac
    17341 agccgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
    17401 gcggtgatgg tactgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcga
    17461 atgagcggca ttttccgtga catctcgttg ctgcacaaac cgaccacaca aatcagcgat
    17521 ttccatgtta ccactcgctt taatgatgat ttcagccgcg cggtactgga ggcagaagtt
    17581 caggtgtacg gcgagctgcg cgatgagctg cgggtgacgg tttctttgtg gcagggtgaa
    17641 acgcaggtcg ccagcggcac cacgcctttc ggcggtgaaa ttatcgatga gcgtggtggt
    17701 tatgccgatc gcgtcacgct acgtctgaac gtcgaaaacc cggcgctgtg gagcgccgaa
    17761 atcccgaata tctatcgtgc ggtggttgaa ctgcacactg ccgacggcac gctgattgaa
    17821 gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
    17881 aacggcaagc cgttgctgat tcgcggcgtt aaccgtcacg agcatcatcc tctgcatggt
    17941 caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
    18001 aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacaccct gtgcgaccgc
    18061 tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
    18121 cgtctgaccg atgatccgcg ctggttaccg gccatgagcg aacgcgtaac gcgaatggtg
    18181 ctgcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcgggccac
    18241 ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg
    18301 cagtatgaag gcggcggagc cgacacctcc gcaaccgata ttatttgccc gatgtacgcg
    18361 cgcgtggatg aagaccagcc ctttccggct gtgccgaaat ggtccatcaa aaaatggctt
    18421 tcgctgcctg gagaactgcg cccgctgatc ctttgcgaat atgcccacgc gatgggtaac
    18481 agtcttggcg gcttcgctaa atactggcag gcatttcgtc agtacccccg tttacagggc
    18541 ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg
    18601 tggtcagctt acggcggtga ttttggcgat acgccaaacg atcgccagtt ctgcatgaac
    18661 ggtctggtct ttgccgaccg cacgccgcat ccggcgctga cggaagcaaa acaccagcag
    18721 cagtttttcc agttccgttt atccgggcga accatcgaag tgaccagcga atacctgttc
    18781 cgtcatagcg ataacgagct cctgcactgg tcggtggcgc tggatggcaa gccgctggca
    18841 agcggtgaaa tgcctctgga tgttgctcca caagataaac agttgattga actgcctgaa
    18901 ctaccgcagc cggagagcgc cggacaactc tggcttacgg tacacgtagt gcaaccgaac
    18961 gcgaccgcat ggtcagaagc cggacacatc agcgcctggc agcaatggcg tctgacggaa
    19021 aacctcagcg tgacactccc ctccgcgccc cacgccatcc cgcaactgac caccagcgaa
    19081 acggattttt gcatcgagct gggtaataaa cgttggcaat ttaaccgcca atcaggcttt
    19141 ctttcacaga tgtggattgg cgatgaaaaa caactgctga cgccgctgcg cgatcagttc
    19201 acccgcgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac
    19261 gcctgggtag aacgctggaa ggcggcgggc cattaccaga ccgaagtggc gttgttgcag
    19321 tgcacggcag atacacttgc cgacgcggtg ctgattacga ccgctcacgc gtggcagcat
    19381 caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg
    19441 gcgattaccg ttgatgttga agtagcgagc gatacaccgc atccggcacg gattggcctg
    19501 acctgccagc tggcgcaggt cgcagagcgg gtaaactggc tcggattagg gccgcaagaa
    19561 aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac
    19621 atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
    19681 aattatggcc cacaccagtg gcgcggcgac ttccagttca atatcagtcg ctacagccaa
    19741 caacaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
    19801 aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtgtcg
    19861 gcggaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa
    19921 taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat
    19981 ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg
    20041 ggggcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt
    20101 gatacaggta ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt
    20161 ggtctgcttt ctgacaaact cgggctgcgc aaatacctgc tgtggattat taccggcatg
    20221 ttagtgatgt ttgcgccgtt ctttattttt atcttcgggc cactattaca atacaacatt
    20281 ttagtaggat cgattgttgg tggtatttat cttggctttt gttttaacgc cggtgcgcca
    20341 gcagtagagg catttatcga gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg
    20401 cggatgtttg gctgtgttgg ctgggcgctg tgtgcctcga ttgtcggcat catgttcacc
    20461 atcaataatc agtttgtttt ctggctgggt tctggctgtg cactcatcct cgccgttttg
    20521 ctctttttcg ccaaaacgga tgcgccctct tccgccacgg ttgccaatgc ggtaggtgcc
    20581 aaccattcgg catttagcct taagctggcg ctggaactgt tcagacagcc aaaactgtgg
    20641 tttttgtcac tgtatgttat cggcgtttcc tgcacctacg atgtttttga ccaacagttt
    20701 gctaatttct ttacttcgtt ctttgccaca ggtgaacagg gtacgcgggt atttggctac
    20761 gtaacgacaa tgggcgaatt acttaacgcc tcgattatgt tcttcgcgcc gctgatcatt
    20821 aatcgcatcg gtgggaaaaa cgccctgctg ctggctggca ctattatgtc tgtacgtatt
    20881 attggctcat cgttcgccac ctcagcgctg gaagtggtta ttctgaaaac gctgcatatg
    20941 tttgaagtac cgttcctgct ggtgggctgc tttaaatata ttaccagcca gtttgaagtg
    21001 cgtttttcag cgacgattta tctggtctgt ttctgcttct ttaagcaact ggcgatgatt
    21061 tttatgtctg tactggcggg caatatgtat gaaagcatcg gtttccaggg cgcttatctg
    21121 gtgctgggtc tggtggcgtt gggcttcacc ttaatttccg tgttcacgct tagcggcccc
    21181 ggtccgcttt ctctactgcg tcgtcaggtg aatgaagtcg cttaagcaat taatgtcgga
    21241 tgcggcgcga gcgccttatc cgaccaacat atcaggacgg aatgatcgca atgaacatgc
    21301 caatgaccga aagaataaaa gcaggcaagc tatttaccga tatgtgcgaa ggcttaccgg
    21361 aaaaaagact tcgtgggaaa acgttaatgt atgagtttaa tcactcgcat ccatcagaag
    21421 ttgaaaaaag agaaagcctg attaaagaaa tgtttgccac ggtaggggaa aacgcctggg
    21481 tagaaccacc cgtccatttc tcttacggtt ccaacatcca tataggccgc aatttttatg
    21541 caaatttcaa tttaaccatt gtagacgact acacggtaac gatcggtgat aacgtattga
    21601 ttgcacccaa tgttactctt tccgttacgg gacaccctgt acaccatgaa ttgagaaaaa
    21661 acggtgagat gtactctttt ccgataacga ttggcaataa cgtctggatc ggaagtcatg
    21721 tggtcattaa tccaggcgtc accatcgggg ataattctgt tattggcgcg ggtagtgtcg
    21781 tcataaaaga cattccacca aacgtcgtgg ctgctggcgt tccttgccgg gttattcgcg
    21841 aaataaacga ccgggataag cactattatt acaaagatta taaagttgaa tcgtcagttt
    21901 aaattataaa aattgcctga tgcgctccgc ttatcaggcc tacaagttca gtgatctacg
    21961 taggccgcat ccaacgtaaa caaagcgcag gaacaagcgt cgcatcatgc ctctttgacc
    22021 cacagctgcg gaaaacgtgc tggtgcaaaa cgcagggtta tgatcatcaa cccaacgacg
    22081 cacagcgcat gaaacgccca gtccatcaag taattgccac tgatactacg cagcacacca
    22141 gaaaaccacg gggcaagccc ggcgatgata aaaccgatcc cctgcataaa cgccaccagt
    22201 ttgccagcaa tagcgggttg cgcagagtga tcgagtgcca gcagcaaaca gagcggaaat
    22261 gcgccgccca gacctaaacc acacaccatc gcccagaaga ccggcaattg cagcggcagc
    22321 cagataaagc cgcagaaacc caccagttgt aacgccagcg ccagcatcaa aagtttgcgc
    22381 cgatcctgat ggcgagccat agcaggcatc agcaatgccc ccgcagcttg cccaagcgtc
    22441 atcaacgcca gcaaggaacc gctgtactgc gcgctggcac caatctcaat atagaaagcg
    22501 ggtaaccagg caatcaggct ggcgtaacca ccgttaatca gaccgaagta aacacccagc
    22561 gtccacgcgc ggggagtaaa taccacgcga accggagtgg ttgttgtctt gtgagaagag
    22621 gcgacctcgc gggcgttttg ccaccaccag gcaaagagcg caacaacggc aggcagcgcc
    22681 caccaggcga gtgtttgata ccaggtttcg ctatgttgaa ctaaccaggg cgtaatggcg
    22741 gcaccaagtc caccgccgcc cattagagcc gcggaccaca gccccatcac cagtggcgta
    22801 cggtgctgaa accgccgctt aattaccgaa ggcatcactg cctgaatgat gccgatcccc
    22861 actccaccaa gcagtgcgct gctaagcagc agcgcacttt gggggtaaag ctcacgcatc
    22921 aatgcaccga cggcaatcag taacaaactg atggcgacgc tgcgacgttc gctgacatgc
    22981 tgatgaagcc agcttccggc cagcgccagc ccgcccatgg taaccaccgg cagagcggtc
    23041 aacagggcag ccacgctaaa gctcattccg cttgcctggc gcaattgcgg tagcagtggc
    23101 ccgacggagg tgagcagtgg tcgcatatta agaccaatca gcaccagaac cagcagcatc
    23161 gtgaacgtct tttgatggtt acgcatggct gtcagaacgg tttggtcggc agatatttac
    23221 catctaaggt gatgaccgca cgttcgccac cctccgggtc cgccactttc ttaacgtcga
    23281 gtttgaagtt aatcgcgctg ataatgccat cgccaaactt ctcatgaacc aacgctttca
    23341 gggttgtgcc gtacacctgc aacatttcat agaaacgata catcgttggg tcggttggaa
    23401 tacggtcatc aatgcagcca cgcagtggaa tcatctgcaa cagtagaatg gcgtcttcgt
    23461 cgagatccag cttcgctccg accaggcggg cggcgtcggc aggaagcgcc tgctgcccca
    23521 gcaaagccgc ggttacaaag gcttctgcca gaccggtgcc gtcggcaatc tcggcaaatg
    23581 agagatcttt tttagctttg ctgagcaaaa tggcatcggc aagatcaaga cgaatattgc
    23641 ggttaatttg cgactgaatc atggtggatc tcctgatggt ttaaaaataa ggttacgctg
    23701 cggtcggttg gcgtagcggt atggcacaaa cgcgaggatt agcggccagt ggcacaaact
    23761 ggcgggttgc gccgtcaaaa gctgtgatgc tgccgctttc aatgtcgtag acccagccgt
    23821 gcagggcaat ccgcccctct tcgagcgcca ggcgcaccga tggatgagtt tgcaaattag
    23881 ccaactgagc aatgacgttt tcacgtacca tcgccgctgc ttttgacggt aaatcgggat
    23941 gcgggcgcgc ctcattaacg acgcgggctg aatcggcata acgcagccag tgggagacgg
    24001 caggcatatg gtccatgcac tgacagctgg caatggaggt catcgcgcca cagttggaat
    24061 gaccgcaaat cacaatgtca gatacccgaa gtgcagcgac ggcatactcc accgaagcag
    24121 aaacgccacc gggttccggc ccgtaggaag ggacgatatt gcccgcgttg cgaataacga
    24181 acagatcgcc aggctcacgt tgcgtcacca gctcagggac cagacggctg tcggagcagg
    24241 agataaaaag ggtgcgcggg ctttgctgtg tcgccagctg tttaaacaag gcttcccgct
    24301 tcggaaatgc ctcgcgctgg aatttaagga atccatcaat aatctctttc accgttaacc
    24361 tctgtctgtc tctggaatga gaggcagact actcgccgat tgtcataagg taaaagactc
    24421 atttatgatg agttctattg gatttactta taggttggcg aatgctctct cgacatatca
    24481 attactttct tgccgtggct gaacatggca gcttcacccg tgcagccagt gcgttgcacg
    24541 tctcccagcc tgcgctttcc cagcagattc gccagttaga ggaaagtttg ggcgtgccgc
    24601 tgtttgaccg tagcgggcga acgattcgtc tcactgatgc aggagaagtc tggcgacagt
    24661 acgccagccg ggcgttacag gaactggggg cgggtaaacg ggcgattcat gatgttgccg
    24721 atctgacgcg aggatcgctg cgtatcgccg tcactcccac ctttacaagc tactttatcg
    24781 gccctttaat ggcggatttc tatgcgcgct accccggcat cacgctccag ctacaggaaa
    24841 tgtcgcagga gaaaatcgaa gatctgcttt gccgcgacga gttggacgtt gggattgcct
    24901 tcgcgcctgt gcattcgccg gagctggagg caattccttt actgacagaa agtttagcgt
    24961 tagtcgtggc gcaacatcat ccgctggctg cctgtgaaca ggtggcgttg agtcgcttgc
    25021 atgatgaaaa actggtcctg ctcagcgcgg aatttgccac cagagagcaa attgaccact
    25081 actgcgagaa agcggggcta catccacagg tggtcattga ggcgaactca attagcgcgg
    25141 ttctggagct gattcgccgc acttcccttt ccacattgtt gccagcagcg attgccacac
    25201 aacatgacgg gcttaaagct atatcccttg ccccgccact actggagaga acgacggttt
    25261 tgttgcggcg gaaaaatagc tggcagacag ccgccgcgaa ggcatttttg cacatggcgt
    25321 tggataaatg cgcggttgtt ggcggaaatg aatcgcggta accactgaca caattgcctg
    25381 atgcgctacg cttatcaggc ctacgagttc agtgctctac gtaggccgga taaggcgttc
    25441 acgccgcatc cggcataaac tacgctcgct ttgtcaacac acagcccagc cgatcaccgt
    25501 ttgtaatcga tggcttctgg ctgctccaga tatacggtgg tttgtgctgg ttgggtgctg
    25561 gcaatcacct taccgccacg taccgaataa cgtacaggaa cctgacggcg cagcgcgtca
    25621 aacccatttt cagccggcag gataatcagg ttggcgctgt ttccggcggc aatgccgtaa
    25681 tcctgcaaat tcaacgtcct ggcactgtgg tgggtgatta aattcaggcc atcgttaatc
    25741 tgcccgtagc ccatcagctg gcaaacatgc agccccatat gcagcacttg cagcatattc
    25801 gccgttccca gcggatacca cggatcgaag acatcatcgt gaccaaagca gacgttaatg
    25861 ccggactcca gcatctcttt aacgcgcgtg atgccgcgac gttttggata ggtatcgaaa
    25921 cgtccttgca gatggatatt gaccagcggg ttggcgacaa agttaatacc ggacattttc
    25981 agcaagcgga acaggcgtga ggtatacgcc ccgttatagg agtgcattgc cgtggtgtgg
    26041 ctggcggtga ctcgcgcgcc cattccttca cggtgcgcca gggcagcaac ggtttcgaca
    26101 aagcgcgact gctcgtcatc gatctcatca cagtgaacgt cgatgagacg gtcgtatttt
    26161 tgcgccaggg cgaaggtttt atgcagcgac tccacgccgt attcacgggt aaattcaaaa
    26221 tgcggaatcg cccccactac gtcagcccct aagcgtaacg cctcttccag caacgcttca
    26281 ccgttgggat acgacaaaat cccttcctgc gggaaggcga cgatttgcag atcaatccac
    26341 ggcgcgactt cctgcttcac ttccagcatt gctttcagcg cagtcagcgt tgcatccgaa
    26401 acatcgacat gggtacgcac atgctgaatg ccgttggcaa tctgccattt cagcgtttgc
    26461 caggcgcgtt gtttcacatc gtcatgggtt aataacgctt tgcgctcggc ccagcgttca
    26521 atgccttcaa acagcgtgcc ggactgattc cagttcggtt gtccggcggt ttgcgtggtg
    26581 tccaggtgaa tatgcggctc cacaaacggc ggtataacta aaccttgttc ggcatccagg
    26641 ctgttttcag ttatgggcat caccccggat tgcgcatcaa tggcgctgat ttttccgtcc
    26701 agcagatgaa tctgccacag cccctctttg ccgggtaacc gggcgttaat aattgtctgt
    26761 aaagcgttat tcgacactgt tagcctccac atgcgtcatt gcagccgtcg ttttgcgatt
    26821 caaaatcggg ttaaggatca gatagctcag cgcgccacct aataccgcgt tgaccggaac
    26881 aatacccggt aaccagtggc ctgcggcaat ccccaaggcg accgccagaa tcgccaccca
    26941 attgacactc atcatacgcg tggtcgcaaa gtgctcatag cggcgacggt tcatcagata
    27001 gtcagcgatg atcacgcccc ctaccggagg aatagctgcc gaaaggaagg tcagccagcc
    27061 gacaaaattg ttatacagcc ataatgcgca gaccgtaccg ataataccgt tgattaccga
    27121 aagggtttta ctcgacatac cagtaatgtt ggcgaaacct aaacccgacg catagagcgc
    27181 gttatcgttg gtggtccaga tattcagccc cagcaccaca atcgcaggca gcagcaggcc
    27241 ctgagcaatc atcacatcag agatatccgc catccccagt gccgcagcgc ccgctgcacc
    27301 gaaaataaac atcaacgagt tgccgaggaa aaaggccacc atcgccacca gcaccgccag
    27361 tttggcatta cgaccaaacc ggacaaagtc agcggtgagc gtgcccgcac tgataaatga
    27421 ccccacaacc agcgccagcg cgacattgaa atctaacggt tgtgcgggaa cgaccgcttt
    27481 taatgcgtcc aggccgccca tgccattaac ggccagccac acggaataac cgccaaggca
    27541 ggcgatagcc ggaaccgcaa tcaccgaaag aaccgtcagc gccgaaatgc caaagaagac
    27601 ggtgacggtc atcagtaaac cggaaacggc aatcagcaaa ttaatatcca gcccggttgc
    27661 cttgcccacc ggaatggcaa acatcgccac gccaacgcca aaccagccaa cctgggtgcc
    27721 gcccagcagc agtgaaggca gccatgagcc tttaacacca aaagagaagc gagcaagaag
    27781 atgagtggtc aggccggttt ttgcgccaat gtaaccaaga aatgaagtgt aaataccgag
    27841 gagaagatta ccgatgagga ctgcgaggaa gaaatcatga tagctaagac cggttccgag
    27901 agtgccgccg gtccacatac tggcggaaaa gaaggttaat cccagcatga cgaacgtcaa
    27961 tgccaatacc cctttccgcg ccgactgcgg gactggcccc tggctaaagt tgttatcttg
    28021 cgacacgaaa ttcccccttt ttgctgtttg aaacccaaaa aatccgccgc attctattca
    28081 tctgaagata aaaagcaatc gttttcgtag gggaatatat ttcttatatg aatgagtaca
    28141 aaaaggcggg ggaaacttat ctgtgtaaaa acagtgggta ggcatgataa gacgcgtcag
    28201 cgtcgcatca ggcatctgcg ca
//
LOCUS       DAZKJR010000061        28214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKJR010000061 DAZKJR010000000
VERSION     DAZKJR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.061"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(69..1466)
                     /gene="ccp"
                     /locus_tag="ACHOWS_002605"
     CDS             complement(69..1466)
                     /gene="ccp"
                     /locus_tag="ACHOWS_002605"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFQ0048157.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1871..3520
                     /locus_tag="ACHOWS_002606"
     CDS             1871..3520
                     /locus_tag="ACHOWS_002606"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFQ0048158.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKACARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3571..4173)
                     /locus_tag="ACHOWS_002607"
     CDS             complement(3571..4173)
                     /locus_tag="ACHOWS_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0048159.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4693..5592
                     /locus_tag="ACHOWS_002608"
     CDS             4693..5592
                     /locus_tag="ACHOWS_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0048160.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5641..6654
                     /gene="yhjD"
                     /locus_tag="ACHOWS_002609"
     CDS             5641..6654
                     /gene="yhjD"
                     /locus_tag="ACHOWS_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFQ0048161.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7047..8369
                     /locus_tag="ACHOWS_002610"
     CDS             7047..8369
                     /locus_tag="ACHOWS_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0048162.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8548..10608)
                     /locus_tag="ACHOWS_002611"
     CDS             complement(8548..10608)
                     /locus_tag="ACHOWS_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFQ0048163.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLASDDNKDTNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10678..11445)
                     /gene="pdeH"
                     /locus_tag="ACHOWS_002612"
     CDS             complement(10678..11445)
                     /gene="pdeH"
                     /locus_tag="ACHOWS_002612"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFQ0048164.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11677..12606
                     /gene="kdgK"
                     /locus_tag="ACHOWS_002613"
     CDS             11677..12606
                     /gene="kdgK"
                     /locus_tag="ACHOWS_002613"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFQ0048165.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSQTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12702..14198)
                     /locus_tag="ACHOWS_002614"
     CDS             complement(12702..14198)
                     /locus_tag="ACHOWS_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0048166.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(14419..15705)
                     /gene="dctA"
                     /locus_tag="ACHOWS_002615"
     CDS             complement(14419..15705)
                     /gene="dctA"
                     /locus_tag="ACHOWS_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFQ0048167.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15888..17876)
                     /gene="hmsP"
                     /locus_tag="ACHOWS_002616"
     CDS             complement(15888..17876)
                     /gene="hmsP"
                     /locus_tag="ACHOWS_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFQ0048168.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17958..21449)
                     /gene="bcsC"
                     /locus_tag="ACHOWS_002617"
     CDS             complement(17958..21449)
                     /gene="bcsC"
                     /locus_tag="ACHOWS_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFQ0048169.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQAGQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQW
                     SSNIQELVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRD
                     YTAARAAYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRR
                     VALAQAQLGDTAAAQQTFNKLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETY
                     KDAMVASGVTTTRPLDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDY
                     WGSSGTGGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGT
                     CTLQDCSGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLG
                     YTVNAHRRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLS
                     GDQLTGKNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGY
                     YSPQEYLSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAA
                     RQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGW
                     QGDMDLPPQPLIPYADW"
     gene            complement(21431..22537)
                     /gene="bcsZ"
                     /locus_tag="ACHOWS_002618"
     CDS             complement(21431..22537)
                     /gene="bcsZ"
                     /locus_tag="ACHOWS_002618"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFQ0048170.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22544..24883)
                     /gene="bcsB"
                     /locus_tag="ACHOWS_002619"
     CDS             complement(22544..24883)
                     /gene="bcsB"
                     /locus_tag="ACHOWS_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFQ0048171.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24894..27512)
                     /gene="bcsA"
                     /locus_tag="ACHOWS_002620"
     CDS             complement(24894..27512)
                     /gene="bcsA"
                     /locus_tag="ACHOWS_002620"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFQ0048172.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27509..>28214)
                     /gene="bcsQ"
                     /locus_tag="ACHOWS_002621"
     CDS             complement(27509..>28214)
                     /gene="bcsQ"
                     /locus_tag="ACHOWS_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFQ0048173.1"
                     /translation="TITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWAR
                     AMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICTGLQQLKASGRY
                     QWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIG
                     SQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANW
                     CLLNYSGLKTPVGSAS"
BASE COUNT         6627 a         7791 c         7116 g         6680 t
ORIGIN      
        1 tattaaaaac agactttatt atcgtaataa aaatcgccca aacactcctg ttcgggcgat
       61 caaattaatt attgtttatc ctgcatatac ggcgtgtaaa ccccgttcag actgtgcagg
      121 aaagctacga tatcatccac atcctcctgc ggcagctctt tgcctacctg atagcgcagc
      181 atcagtttca ccgccccgtc cagcgtcggc acgtcaccgc gatggaagta cggtgccgtt
      241 aaagcaacgt tacgtaaacc gggtactttc tgacgcaatt tatcacgctc ttctttagta
      301 acattcatac gaccaatatc cgccgccgta atttcaccaa agttaaagtc ttttttcagc
      361 cccaacggtt caaaggaacg tccgccgaga ataataccac catgacaagt tgcacattta
      421 ttatctttaa ataattgata gccttttttc tgttgcgccg tcagagcatt ctcatctcca
      481 cgcaaccatt tatcaaatgg ggaatccggc gtaattaatg ttttctcaaa ttcagcaatg
      541 gcatcagtaa tattttcgcc actgaaacct tgtggataga cttcgaggaa ctgcgcttta
      601 agctgcggat ctttttccag cttagcaata atttcgtccc aggatttcga cgccatttca
      661 atcgggttca acggcggtcc accagcctga tcctgcaatg ttgccgcacg accatcccag
      721 aactgctcaa cgttaaatac tgagttaaat accgtcggcg cgttaatcgg cccaactgcg
      781 ccaccaacac caatcgatgt ttttctgcca tcgacgcccc ccgcattcaa cgcatggcaa
      841 tgagcgcatg aaatggtgct atcagccgat aaacggggat cgtgatacag cgcaaaaccc
      901 aacgccactt tttgcgcatc ggtaggcagt ttttgcggga tgggctgcac cggttcattg
      961 cgatgctccg gagcagtatc attgctggcg taatattccg cgcgctgttt tgcaatccag
     1021 gccagtattt ccgcccgctc ttcatcactc accttacccg cccagtgtag cgcggtataa
     1081 cgcgttggcg gcatagtttc atactgcatc acccattcaa tcttattcag atcgctttgc
     1141 gaaacgggtt tattagccag cagagccgca cgcacggcct cgaggttaaa agatttatat
     1201 ccaagcttta tgtcgtaatc catcaactgt ttcgcgccgg gaatataata ataggcgggt
     1261 aattctgccg aaggtgtatg gcaatagtcg catccttttt cacggagaaa gcctaaaacc
     1321 ttattatttt cactgacagc agatgcctga acatcggcct gtttactgcg tttattatcg
     1381 tggtaccaca cataaccaga taaccctaaa tagcaaatcg cgacgccagc caggccgatc
     1441 gcggtaatac gtgagaccat tttcattatt cttccctcac ggtggtgagt tattgtgacc
     1501 cagaaaattt tccagaatgc tgcaaacgtc tctgcgagac aaagccatca taaaaaacag
     1561 ggagatatgc tttttgatag gaatcaatca attacatgaa tataaactat caataagata
     1621 gccagtgcct attacccaga tcaggaatgg catatcatga ttgcggcgag agcaggattg
     1681 aatgttctga aaatgaaaaa caacacgaaa tccgtatttt tataaattcc attgatatta
     1741 gtgcgttata ttttttggca ggttaataaa cgtgatccac cgcacgcttt gtcgcccacc
     1801 aggcggagcg aatgactacc cttaaagaaa agcccgataa ttagcgacga atttcggagg
     1861 tcggatcctt atgctcaatc agaaaattca aaaccctaat ccagacgaac tgatgatcga
     1921 ggtcgatctc tgctatgaac tggacccata tgaattaaaa ctggatgaga tgatcgaggc
     1981 agaaccggaa cccgagatga ttgaagggct gcccgcctct gatgcgctga cgcctgccga
     2041 tcgctatctc gaactgttcg aacatgttca gtcggcgaaa attttccccg acagtaaaac
     2101 ctttcccgac tgcgcaccta aaatggaccc gctggatatc ttaatccgct accgtaaagt
     2161 gcgccgtcat cgtgattttg acttgcgcaa gtttgttgaa aaccacttct ggctgccgga
     2221 ggtctactcc agcgagtatg tatcggaccc gcaaaattcc ctgaaagagc atatcgacca
     2281 gctgtggccg gtgctaaccc gcgaaccgca ggatcacatt ccgtggtctt ctctgctggc
     2341 gctgccgcag tcatatattg tcccgggcgg ccgttttagc gaaacctact attgggattc
     2401 ctatttcacc atgctggggc tggcggaaag tggtcgggaa gatttgctga aatgcatggc
     2461 cgataacttc gcctggatga tcgaaaacta cggtcacatc cccaacggca accgcaccta
     2521 ttatttgagc cgctcgcaac caccggtttt tgcgctgatg gtggagttgt ttgaagaaga
     2581 tggtgtacgc ggtgcgcgcc gctatctcga ccacctgaaa atggaatatg ccttctggat
     2641 ggacggtgca gaatcgttga tccctaatca ggcctatcgc catgttgtgc ggatgccgga
     2701 cggatcgctg ctcaaccgtt actgggacga tcgcgacaca ccgcgtgacg aatcctggct
     2761 tgaggacgtt gaaaccgcga aacattctgg tcgcccgccc aacgaggtgt accgcgattt
     2821 acgcgcgggg gcggcctccg gttgggatta ctcttcccgc tggctgcgtg atactggtcg
     2881 tctggcgagc attcgtacca cccagttcat ccccatcgat ctgaatgcct tcctgtttaa
     2941 actggagagc gccatcgcca acatctcggc gctgaaaggc gagaaagaga cagaagcact
     3001 gttccgccag aaggcctgtg cccgtcgcga tgcggtaaac cgttacctct gggatgatga
     3061 aaacggtatc taccgcgatt acgactggcg acgcgagcaa ctggcgctgt tttccgctgc
     3121 cgccattgtg ccgctctatg tcggtatggc gaaccatgaa caggccgatc gtctggcaaa
     3181 cgccgtgcgc agccgattac tgacacctgg cgggattctg gcaagcgagt acgaaaccgg
     3241 tgaacagtgg gataaaccca acggctgggc accgttacaa tggatggcga ttcagggatt
     3301 taaaatgtat ggcgatgacc ttctgggtga tgaaatcgcg cgcagctggc tgaagacggt
     3361 gaatcagttc tatctggaac agcacaaact gatcgagaaa taccatattg ccgatggtgt
     3421 tccccgcgaa ggcggcggtg gcgagtatcc gttgcaggat gggtttggct ggactaacgg
     3481 tgtggtgcgc cgtttaattg gtttgtacgg tgaaccataa tatttttaca gccagccgct
     3541 aacttcctgc tggctgtaaa attatcctct tcaggaggag atatttaaca tcattgccgc
     3601 ctgggtgcga tttttcactt ccagacggcg atacagggat tccagatgcg cttttaccgt
     3661 tccggtactg atattcagcg ccctgccgat ctccttattt gattcgcccg ccgctaacat
     3721 ggttaaaatc tcccgctggc gggcgcttaa cgatttgaga tctttaatgt ccttttccgg
     3781 cgtcgtccgc cagtctccag gcagaaacat catccccatc gccgcactat ttaccgccaa
     3841 cgcaaatgtc tcgacggttg aatcacgagg cacaatggcc agcacattaa aatggataac
     3901 ttcctgtaac caccgtttat tgcaatccgt cgccgtaatt aacaccttaa cctcaggaaa
     3961 ttgcaccacg gttttttgca gcaaccagta acaaaactca ccatcctgat cgccatcgag
     4021 catcactaag gcttcagggt aactttccag cttttgccat aactcgtctg cctgactggc
     4081 cccctgaata ctcactcctg gaatacgttg ctgtaaactg attttcattc catgaataaa
     4141 tattgactgc ctgtcaaaca tgactatttg cataactgaa tctccacctg aatacgttaa
     4201 aaagacttaa gtagtggaag ggtattaccc gcgagaaaaa ataagaattc gccatttggc
     4261 ggtggccatt ctacagagat gacgcgtaga aaatagttac cgatataaat agttacagct
     4321 aaacgcctga aattacatgt cgagggcact atttaaaaca attttgagga tttccttata
     4381 ttagtggtta gtacgcatgc aattaaaaat gaaattccgc gaccacaagc caaaataaca
     4441 aatggcaatg agacaaaaat aagcacaaat agccaatacg tcctctattc actttaaagg
     4501 gaatcgctga aaaatacgct ctgtttaagg ggattcacct ttctaagaaa gctattcctc
     4561 ccttttccta ctgagaaatc gccacattcg tcatgacaac attgtgaaac ccggctttag
     4621 atgttagaaa aaacaataaa caatgcgata tgcgcgttac tgtttcgctg tgaacaacaa
     4681 tcggtcaaag aaatggataa aattcacgca atgcagttgt tcatcaaagt cgcggagctg
     4741 gaaagttttt cccgcgcggc ggatttcttt gctttgccaa agggaagtgt ttcgcgccag
     4801 atacaggcac tggaacatca acttggcacc cagcttctcc agcgcactac gcgacgggtc
     4861 aaactcacgc cagaaggcat gacctattat caacgggcaa aagatgtgtt gagtaatctc
     4921 agcgaactgg acggtctgtt tcaacaggat gccaccagta tcagcggtaa attacgcatc
     4981 gacatcccgc caggaatcgc gaaaagcctg ttactgccgc gcttgtcgga atttctctat
     5041 ctgcatccgg gaattgagct ggaactgagt agccatgacc gtccggtaga tattcttcat
     5101 gatggttttg attgcgtgat acgcactggc gcattaccgg aagatggcgt tatcgcccgt
     5161 tctctcggca aactgaccat ggtcaactgt gccagtccac actatctgac gcgctttggt
     5221 tatccgcaaa gccccgatga tctgacttca cacgcaatag tgcgttatac accgcacctg
     5281 ggtgtacatc cgttaggttt tgaggttgcc agcgttaatg gcgtccagtg gtttaagtct
     5341 ggcggcatgt tgacggtaaa cagtcgcgaa aactatctcg ccgccggtct tgccggtctg
     5401 gggattattc agatcccgcg cattgccgtg cgcgaagccc tgcgtgccgg gcggcttatt
     5461 gaagtgttgc ctggctaccg tgccgagccg ctctcccttt cactggttta tccgcagcgt
     5521 cgggagcttt cccggcgtgt aaacctgttt atgcagtggc tggctggcgt aatgaaagag
     5581 cacctggact gaccgactat actttttaag aatgaccacg acagaaggac aaaagagcgg
     5641 atgacgcagg aaaacgagat caaacgtccc acccaggatc tggagcacga gccgattaag
     5701 cagttggata atagcgagaa aggcggcaaa gttagccagg cgctggaaac cgtcaccact
     5761 actgccgaga aagtccaacg ccaaccggtt attgcgcacc tgattcgcgc aacagagcgc
     5821 tttaacgatc ggctgggtaa ccagtttggt gcggctatca cctatttctc atttttgtcg
     5881 atgatcccga ttttgatggt atcgtttgcc gcagggggct ttgtgctggc ctcccatccg
     5941 atgttgctac aggatatctt cgacaaaatt ctgcaaaaca tcagcgatcc gacgctagcc
     6001 gccacgttga aaaacaccat caacaccgcc gttcagcagc gtactaccgt agggcttgtc
     6061 ggcctggcgg tggcgcttta ttccggcatc aactggatgg gtaacctgcg tgaagcgatt
     6121 cgcgcccagt cacgcgatgt ctgggaacgc tcgccgcaag atcaggagaa gttctgggta
     6181 aaatatctgc gtgattttat ttcgctgatt ggtttgttga ttgcgctgat tgtgacgctt
     6241 tcgatcacct cggttgccgg ttcggcgcag caaatgatta ttagcgccct gcacctgaac
     6301 agcattgagt ggctgaaacc gacgtggcgg ttgattggcc tggcgatttc catcttcgcc
     6361 aactatctgc ttttcttttg gatcttctgg cgcttgccgc gccaccgtcc gcgcaaaaaa
     6421 gccctgattc gcggaacatt tctggctgcg attggttttg aagtgattaa aatcgtgatg
     6481 acctacaccc tgccatcgct gatgaaatcc ccctctggcg cagcatttgg ttccgtgctg
     6541 ggactgatgg catttttcta cttcttcgcc cgtttgacgc tgttttgcgc ggcgtggatt
     6601 gccaccgccg aatataaaga cgacccgcga atgccgggga aaacgcagcc ttaaaatagg
     6661 ccggatgcgg cgtacgaatt tattcggcct ggctccccgt aggccggata agatgcgcca
     6721 gcatcgcatc cggctataat gcgcacataa cctcttgaaa ctctttccca gagcctcttt
     6781 agccatctat tcaggagcaa acaatttcat accaactcat aaccccagca tataaatcca
     6841 gttggtaact tttatttaac ctgaaaccag ttttatccac tatttataaa attatgtgaa
     6901 gcatttcata gaagaaaaat cactggccta aacattatcc cctttttgcc tggtttttga
     6961 ccatttccgc gatttgttac acattgaaat atcacttttg ctgtgcgtaa tatggctatt
     7021 cgttagccaa aaaataagaa aagattatgc aagcaacagc cacaacactc gaccacgagc
     7081 aagaatacac gccgatcaac tcgcgtaata aagtccttgt cgcctctctc attggcacgg
     7141 ccattgagtt cttcgacttt tacatttacg ccactgcggc cgttattgtg tttccgcata
     7201 tcttcttccc gcagggcgat cctacggcag caacgctaca gtcgctcgcc acctttgcca
     7261 tcgccttcgt cgcgcgcccc attggctctg ccgtttttgg tcattttggc gatcgcgttg
     7321 ggcgtaaagc gacgctggtc gcctcgttgc taacgatggg gatttcgacc gtggtgattg
     7381 gcctgctgcc aggctatgcc acgattggta ttttcgcccc gctgctgctg gcgctggctc
     7441 gatttggtca gggtctgggc ttaggtggtg aatggggcgg cgcggctctg ctggcgactg
     7501 aaaacgcccc accgcgcaaa cgtgcattgt acggctcctt cccgcagctg ggcgcaccga
     7561 ttggcttctt ctttgccaac ggcactttct tgctgctttc ctggctactg accgacgagc
     7621 agtttatgag ctggggctgg cgtgtgccat ttatcttctc ggcggtgctg gtcattatcg
     7681 gcctgtatgt tcgcgtgtcg ctgcatgagt cgccggtgtt tgagaaagtc gctaaagcga
     7741 aaaaacaggt gaagatcccg ctgggtacgc tgctgaccaa acatgttcgc gtaaccgtac
     7801 tgggtacgtt cattatgctg gcaacctata cgctgtttta catcatgacg gtctactcta
     7861 tgacctttag taccgccgcc gcgcccgttg ggcttggcct gccgcgtaac gaagtgttgt
     7921 ggatgctgat gatggcagtt attggttttg gcgtgatggt gccagtcgcc ggattactgg
     7981 ctgatgcctt tggtcgccgt aaaagcatgg taatcatcac cacgctgatc atcctgttcg
     8041 cgctgttcgc ctttaacccg ttgctcggtt ctggcaaccc gattctggtt tttgccttcc
     8101 tgctgctggg gttaagtctg atggggctga ccttcggtcc gatgggcgcg ctgttaccag
     8161 agctgttccc gacagaagtg cgttacaccg gagcatcgtt ctcttacaac gtagcgtcga
     8221 ttctcggggc ttccgttgcg ccgtatatcg cagcctggtt gcagactaac tacgggttag
     8281 gtgcggtggg gttatatctg gcggcgatgg ctggcttgac gttaatcgcc ctgctgctga
     8341 cccatgagac gcgacatcag tcgttgtaat aagagtgaaa agcctgatgc gctacgctta
     8401 tcaggccaga atttccatac aatatattga attagcaagg atttgtaagc cggaggcgtt
     8461 cacgccgcat ccggcatgaa caaagcacac tttgccaaca atctaaatct gtttaattgc
     8521 cggatgtcag acatccggca attatcatca cttcttcatc tgcgacaaaa ttgtcctgca
     8581 ctgattagcc tccccttcgg aaggtgagat cagcgccagt aacgccgccg ccggtgttac
     8641 cagcgttgcc agtgccgccg caacggctcc gcgagcaatc aacggcccgg ctttcacccc
     8701 agcctgcgga tttttaaacg tcccacgtac atacagcggc gaacgcaatg tgataatccg
     8761 aatcccttta ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt
     8821 accggtaacg ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac
     8881 gccgttggca atatccagat tcgccgccgc acagttcacc cgcacctcat catcaccaaa
     8941 tatcgcaccg acaatgtagt taccgacatt cagcccgaca atctccatca ggttgcggct
     9001 caccagcccg tcattcatca gcagtttcag gttgccgtta ctgttgccta aaagcgccgc
     9061 caccgagtta ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg
     9121 catcagttct acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc
     9181 ccgcccttgc atcggttttt tatcaccttc cagatgaata ttcgccgcaa tgctgccgcc
     9241 tgccatgcca aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt
     9301 agaaagatcg ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac
     9361 atcggcatcc atgacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc
     9421 cgcaggctga acgctttttt cacccttctt ctgttcagac cgtttcgact tttcagctcc
     9481 tttcccggaa tcaacgccaa ttaacggccc taaatctgcc agccgcaatt gccgcgactc
     9541 aacatcacct tccagttttg gtcgtggctt gccggtggtg tagaccagag aaccgtggat
     9601 atcgctatcg ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt
     9661 gtcgattttc gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac
     9721 gcccgtcagt tcatagagat cacccagcga atcgccagaa aatttaagcc gtaaatcgac
     9781 accacccatc ttcattgggt cattcacaac gccatcaaaa gcaacacggg tgttaccaga
     9841 gcgaaaatca gcctgtacag gaaatggcgt cccttcgcca cgcagcgcca gcataccgcc
     9901 tatttttccc gtaccagtga gcggctctcc gttataacgt ccctgcgcct tcaggccaaa
     9961 aacgtaatcg cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa
    10021 cggcagcggc ttgcctaacg gatcgacaaa aatttctaga tccgctttgc ttactttgtc
    10081 atcaatggcg atccgccctt gatcgaacag aatattatcc agccgaaacg accatgccga
    10141 cggctttgca ttcgtgtctt tgttgtcatc gctggcgaga ttaaatgtcc agttgttgtt
    10201 cttttctgag aggcgaatca ggcgtgcgtc gggcttttcg agcttgatcc acggcaacca
    10261 gaccgttttg gtcagcagcg ccagcggggc cagcgtcgct tctactcgcg gcaaatgcac
    10321 cattgtgact tcgggaatat ccggtggatt gccaagaatg atgtcttccg cgtgtacatg
    10381 gggccacggc acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc
    10441 cagatcgcca cggatagcga acggacgatt caactccgca gagactttct ggttgatggt
    10501 cggtttgagt cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa
    10561 caagaaagcc cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct
    10621 catattcttc ctgtgccagt cctaaagata gtccagccag gcggaaaatg aggcagctta
    10681 tagcgccaga accgccgtat tcagcgtttc tatcggtgcc gggcgtgaaa gaaaccagcc
    10741 ttgtgcggcg aatgcgggcg agttctgaac atcacgccac tcttccggcg tttctacgcc
    10801 ctcgacaatc accccgcgac aatagcgatt catgagatgt aaaagctgag aaaagagtgt
    10861 gcggccttcc ggcgactgac gcagcatcac gaacagctct cgcgcgattt tgatgtagtc
    10921 ataacgcact tcacttagcg cagagaaatt tgccatcccg gtgccaaaat catccagcca
    10981 cagcgggcca aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc
    11041 caccagttcg aaacgcagcc agggaagacg ctcaatttgg cgcaggattt ttggttgctg
    11101 acgcagggcg atgagcgtag ggccatcaat attgaccgat gccagcaggt cgtgctctat
    11161 aaagaagtcg gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg
    11221 gctaacggtg atttcagtaa aatagcgatc cggcggcagg cgttgcgaag ggttcaaggg
    11281 atgcgtgacc accgttaata gctccacggc cattaaccgt ccgcatgttt gatagatcgg
    11341 ctgccaggtg taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat
    11401 gcttgcttca gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg
    11461 tttaaaagtg atgctgcccg gactcgacaa agattatcgg cgcgcaaggg cagaacttta
    11521 ccgcctggtc ggggtaaatt tccccactcc acaacggaat ctttcaggaa tacgttctgg
    11581 ctcaaaaaaa taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa
    11641 actaacgcta attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt
    11701 ggcgaatgca tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc
    11761 gataccctga acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt
    11821 cattacgtaa cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac
    11881 ggcgagaacg ttgatacttc cctgacccaa cggatggaaa accgtctgcc gggcctttac
    11941 tacattgaaa ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc
    12001 gccaaattct ggctggagag tgagcaatcc gcggcgattt gcgaagagct ggcgaatttc
    12061 gattatctct acctgagcgg gattagcctg gcgatcttaa gccagaccag ccgcgaaaag
    12121 ctgctttccc tgctgcgcga atgccgcgcc aacggcggaa aagtgatttt cgacaataac
    12181 tatcgtccgc gcctgtgggc cagcaaagaa gagacacagc aggtgtacca acaaatgctg
    12241 gaatgcacgg atatcgcctt cctgacgctg gacgatgaag acgcgctgtg gggtcaacag
    12301 ccggtggaag acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa
    12361 cgcggggcgg attcttgcct ggtgtccatt gctggcgaag ggttagtgga tgttccggcg
    12421 gtaaaactgc cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagtgcc
    12481 ggttatctgg cggtacgttt gacgggcggc agtgcggaag acgcagcgaa acgcgggcac
    12541 ctgaccgcaa gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga agcgatgcca
    12601 gcgtaaaagc agcatatgca aaaggctgga tagagtgtta cccccctatc cagctcatcc
    12661 ataaatacct gatggttgtg ctaactcatc accatcaggc attactgtac aggtggaata
    12721 tccgtcactt caggatgtac gtcatccgtg gcgacagatg tggcggcagc cgcagtcgat
    12781 ggggccatga tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct
    12841 ttcggctgca gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt
    12901 aacatctcca ccgtcaggct gttaaggaaa tcctgacgca gtttctgata ttgctccggc
    12961 gcgatatcga cgacctgatt ttgcaacgaa cgcatccgct gccccatcag aatatcggta
    13021 tcagctcgcg cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac
    13081 gcattgaact cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc
    13141 agattaaggt tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca
    13201 cgcagataca gcacacgaca gtcaaatcca agaccgatgt ctttactgtt actggcgctt
    13261 aacgcttgct gaacatgcca gaacagcgcc tcacgggcca ggtccgcacg ccagtagcgc
    13321 agcagtgcgg ctgattcacg aatcggctgc cacggcgtat cccacatgat tgataaccgg
    13381 tcctgacgca ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc
    13441 ggcaccggag ccggtgtttc acgtttgcct ttcagttcgc caaacgtttt attgatttgg
    13501 tcgacgaccg agcgcgcatc cacgtttccc accaccagca gcgtcattgc atccggggta
    13561 taccatttct gatagaaatc tttaatcttt tccgcttcaa cgggttgttt cagcggatcg
    13621 gcaggatcgt gacctaacaa ggttgatcct ttcagacgat agcgccacca gccctcttta
    13681 gtatcggcag gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct
    13741 ggtgtgatgg tcagtttgcc agtggcattt gccagataag agagcgcttc tttcagcaag
    13801 tcgttacggt tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattact
    13861 ggcggcatcg ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgct
    13921 tcaaggccac cgctttgcgt taacgcaata cgagggatgg cgtggctgta accgctctgt
    13981 tgtgtacttt cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgcta
    14041 ggacgctggg gcgtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc
    14101 catgctggat caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc
    14161 aaaccgcccg ctaaaagtcg aatttttgtg ccctgcatgt gaacccctga tcaactatcc
    14221 tggtaataaa aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt
    14281 tagaccgcaa gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg
    14341 tggtaagttc aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg
    14401 cgggcataag tgatgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt
    14461 attcagcaca tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat
    14521 ggtcgctacg ccgttaccga ccaggttagt cagcgcacga gcttctgaca taaagcggtc
    14581 gataccaagg atcagcgcca gacctgctac cggcaaatgg cccaccgcag agagcgtcgc
    14641 cgccagcacg ataaagccac tgcccgttac cccggcagcc cctttggaag agagcaacaa
    14701 cacaattaat agcgtaattt ggtggacgat atccatctga ctgttagtcg cctgggcgat
    14761 aaacaccgcc gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt
    14821 cgggatgacc agccccacca ccgatttccg gcagccgagt ttctccatct tgtcgagcat
    14881 acgcggcagc gccgactcgg aagaagaagt gcccagtaca atcagcagct cttcacggat
    14941 gtagcggata aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag
    15001 caccacaaac aggatacagg tgatgtagaa acagataatc agctgcccca gttgcaccag
    15061 tgtgccgacg ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac caataggtgc
    15121 cagacgcatg atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatgacgtt
    15181 aaaaatcagt tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa
    15241 cagcagcacc tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac
    15301 atccataatg aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc
    15361 tttcgcatca agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac
    15421 gatgataaga ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac
    15481 tgcgccggta cgaccaaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt
    15541 acaaaagatg acaggagcga tgatcatctt aatgagctta acgaacccgt cgccaagcgg
    15601 tttcatttgc tcgcctattt cagggtagaa atggccaagg agaataccaa tggctatcgc
    15661 tgtcaggacc tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt
    15721 aaaaccacag gtcttgtaag gttatggggt acctgcggcc ctaaaataac acccagacaa
    15781 catcacagaa atgtacctgg atcataattg aaacaaaaag gttaaaaagt ttgagctggc
    15841 tcgcacaaac cagcactttt taaagttttg taatcagttt ggggtagcta cttttcttcc
    15901 aggtaactct cttcgaagat ttcaataggg agtgggcgag caaaaaggaa gccctgggca
    15961 ataccaacgc ccgcttttgc cagccagtcg cgttgtgctt cagtctccac gccttcggca
    16021 atcatttgta agttcaggct ttgcgccaac atgataattg cagcaatcat gctgctatct
    16081 tccggcaagc cttcaacaaa cattttgtcg attttcagta cgtcgattgg caacgatttc
    16141 atatgctgca gctgacgcag ccctgcgtag cccatgccga aatcatccag cgccacccga
    16201 actccggcat tgcgcagcgg acggaggatt gccaccgcag catgagggtc gtcaatacgt
    16261 cggctttctg tcacttccag aatcagtgtt cccggctgaa tgcgatagcg ggttaacagt
    16321 tccagcatat ccgctaccat attcgggtgc atcagttgta gcgctgagag gtttaccgac
    16381 aagggcagca taatgccgcg ctcttgccag gcagcaagca gtcgacaaga ctcttccagc
    16441 acccagtgac caacggtaac catcagccca cagcactcaa tgcgatcgat taagccatcc
    16501 ggcagatccc aactgccatc cggttgctgg atacgcagta acacttccgc actgaccagt
    16561 ttaccgctgg tcatctcgac ctgtggctgt aaccaaatcg caaattgatg attttccagt
    16621 gcattaagga tatcgctctc ttccgtcaac cgctgctggg cggcttccat ctgctgcgga
    16681 tcaaagaact gaatctgatt tttgccttta tggcgagcgg taaatgccgc agaaatagcg
    16741 cgactgtaaa gctgttcggc ggtgagatcg ccgtagaaca tcgccacgcc aatgctacag
    16801 tgcggacgga gttgaatacg ttcaatcggc aggcgctcgc tcatgatagt gagcacttgc
    16861 tgacctaatg tgatagcgtg ccacggttcc tgtacaccgt tggcaatgac agcaaagtca
    16921 taaccgctaa tctgcgcgag gatcatgcgt ggcgacagta ccgatttgag tttttccacc
    16981 agcgtcagca gcagaatttc tcgttgcgcc tctttcagca cgcccgcagt atcacgcagg
    17041 gtttcacagg tgatgatcat cagcgcggtg gtttgtttac gcgcgacaac ctgctccagc
    17101 atctccatca gcaaggcttt gttcggcaaa tccgacaccg ggaagcgcat cgcgttttcg
    17161 ttctgttctt cataatggcg ctgcagcaat tgctggttga ggttgtaact gcgcaccagc
    17221 ataccgattt catcgtcctg atgcagacgt ggtaatgcca gttggtgacc aacaagctcc
    17281 tgggctggga tggcgttaag ttcgcgagca atattgcgta acggatgtaa aatcagtcga
    17341 ttaatacacc agctgatggc gacggtcagg ataagcgaca aaagtaagta aatggtcact
    17401 aacgttgaga gggtgctcat cacgaactta tacatacgga aggaatccgc ctgtagcacc
    17461 agataggcaa ttggctgcgg atttgccgga cgttcgagcg agtagacgcc cagcgagatt
    17521 tgaaccggta gctcaaacag gcgagtaacc attaccggca ccggacgctc tggaataaaa
    17581 cttttacgca gcgcctggaa ctggttgggc agcaccacat cggcacggct gaccacgcct
    17641 gccggtttaa tgctggcaag aatagcttcc gcttcgggaa tatcgccttt caaaatagct
    17701 gaagataagg gttcgcggac agagcgagca atgctttcca gttgcgtagc cgtgttatag
    17761 cgattctgct ggaccagatg gaacagcaaa acggtgcaaa aaataaaaac gaacaccagg
    17821 acaacggctg ccaccattgc catctgcttg attgttaacg agcgacttac gcgcaaattg
    17881 tctccccccg gaaatcccag attgccgccc gagtataccc gatcgcagcg gcattaagag
    17941 aggcgctatc tgaaaactta ccagtcggcg taaggtatca gcggctgcgg cggtaaatcc
    18001 atgtcaccct gccatccggc ggcggaataa cgtacgtaga gcaggaaatg gctgggtgcg
    18061 taatctttcg cctgctggat atcaattgcc gtgccgacaa accagttgga agtaacacgt
    18121 cgttcaagta atgcccgcgc cgtatagccg aagccctgac tgctgccgcc atcgttggat
    18181 tgccgcgcag cttcttcctg ccagtcggtc gggatcagat tcatcagcgg ataacgcggc
    18241 atggttttgg tgcgtgaatg cgaccacgag ccagacgccc ccagctccca cgaccagttt
    18301 tccgtgcgct cccgccacat caccggtatg gcaaacgaca ggtattcctg cgggctgtag
    18361 taaccgccct gaccgagtga gtagccactc aggtctttgt cgtaatgcca gatcatgttg
    18421 ttcaggccga ttgtgacgcg gcgattgttc tggttaatga ccttatagta atagcccgtc
    18481 atccagcgca cacgccagtt atcttcgaca tttttaccgg ttaactggtc gccactaagc
    18541 gatgcccaga cgccgtttgc ttcaccttta tcgtagctca ggcttagccc cacgccgtcg
    18601 gcacgcacgc cgccccattt tttcccggta ttgctcgggg agtctttttg cccaccaaag
    18661 gccagcaaag aactggagat gggccgacgg tgggcgttaa cggtgtaacc cagcggcccg
    18721 atatcatcgc tgtaactgat gccgccgacc acatccacca cgttgaagcc catcggcgtg
    18781 gtgccgatat cccagctcca ggtgtcgttg cgccaaccga ccgccacgct ggcaccggaa
    18841 tccgactggc tgcggttgcc gctacagtcc tgtaatgtac aggtgcccca gttgtcatcc
    18901 catttaccat cggcattagt ggagaaactg ccgacgttca tattgacgaa atcactgcga
    18961 aagaacatcc gcccgtcaga atacggcgca tccacctgca acatggtagt gtgcgctttc
    19021 agatcggagt aaccaccggt gccgctcgaa ccccagtaat catgctcaag ggtgacgtta
    19081 agatcctgct ggcgatagag gtccgccgca tcgctgcgca cgccgcgttt cagccagtca
    19141 tctttctcgt cattgcgggt cagtcgggta aaggtgtcgt tatccagcgg acgcgtcgtg
    19201 gtcacaccgg atgcaaccat cgcgtctttg taggtttcca gcgcctgcgt tggatcgccc
    19261 gcctgcgctt caaacttcgc accatcacgc agtaccatcg cactttccat cgacggcggc
    19321 tgagattttg ccagcgggat caacttatta aacgtctgct gcgccgctgc ggtatcgcca
    19381 agctgcgcct gtgccagcgc cacgcgccgc tgagtgttca gcgaggcgtt atcggtggca
    19441 ggcagcttcg ccagctggct acgtgccgcc gctttgtcac cggcagcaat atccacttcc
    19501 gtcagaccaa gaatggcatc ggcgttagtt ggctcccgcg tcaggacatt ctgatatgca
    19561 gcgcgagcgg cggtgtaatc acgtcgttgt tgcgcccagt cagccagcgt gaggtctatg
    19621 cgcgtggaag gtggttgctg gcgcagcatc gcttccgctt cagcttcttt gccgctttct
    19681 cgcaggcgat tagcggtttc caacacctga tcgctttgca gtcgattaac cagctcctga
    19741 atattgctgc tccactgcgc gcgcggcagg ctgttgatat gcgccagcgc cgctctgtcc
    19801 tggtcatgac cagagagata cagcccgtaa gcgtaaacct gctccgggtc gttcggcttc
    19861 tgctgcgcca gattacgcat taacgtatcg gcctggctgc gttgtccggc ctggctgcgt
    19921 tgtccggcct gccagagatc ctgtgaaagt cggtaagtaa tccatacact gccggggtcc
    19981 agcgccagtc gttgccgctg aagtgctgcc gcctgcgccc atttcccctg gttttccagt
    20041 gcctctgcct gctgtgccag acggtcgttt tgcaggctgc gttcgatatc atcaatgctg
    20101 cgccgctggc tggcagagag cgaggcgata tacgcttcgg ctttttctgg cgactgctgg
    20161 cggtaaatat tcgccagccc gcgcacggcg ttagtgttgc cgctgtccat acgcaaggtc
    20221 tgctggtaat agcgttcggc ggcgggataa tctttgcgcg ccatcgccac atcgcccagc
    20281 ccaagcactg catagctgtc ggtgttatcg acattacgtg cctgctggaa cagccgttct
    20341 gcccggtcag gattattggc tttcagcgcg gcatcgccct gctggatcgc cagccagtag
    20401 cggtttactt tcagcagact gttccatttg tcgttattgc tgttgtgcgg atccagcgcg
    20461 agggcttttt ccagattcgc caccgcatta gcgcgatcgc ctttctgaga atacgcctgg
    20521 cccagcgccc ccagagcctc actgtctttc gggttcgccc gcaccgcctg ttgtagttcg
    20581 ggaatggctt tacccgccat gccagagtcc accgccgcta aaccttgcgc acgagcgcgg
    20641 aaagcagggt cggccagctg tttttgctgt tctgccagtt gcgattgtgc agcagctacg
    20701 ctatcgccat cgctaaagat cgagagatat tttttcagtg cctgaacgct ggcatcgctg
    20761 acaggcatgt ctttaatctg cccgtaccag attttagagg ccccttcgcg cccggcgttc
    20821 gatttcgcca tctgttccag gacggcaaaa ccttcatcac ggcgatcgct actaaacagt
    20881 aatagcgcca gattgttttg caggcccgtg ttgcccggcg tatccgcatt aatgcgtttt
    20941 agctgattaa tcgcttcgcc acgtcgggcc ggaattttcg ccaccgtact ccagtactcg
    21001 acagcaatgt caccttccgg cggcgcaccg ttgaacagtt tgttgtaact cgccacagct
    21061 tcttccgcat gaccagtcgt cgcctgcaat cttgcctgtt gcagtgcctg acgaccatcc
    21121 gacgtggaaa gcagcatcgt agtccgtgac gatttatacg catttgaact cggcgctaac
    21181 tgcgacagcc gatcgagctg tttttgtgcg ccatcaatat cgccctgacg taacagagaa
    21241 cgaaaacggg cagcaacgac gtccgggtta ttcggatcaa taagttccag acgatacaac
    21301 gactgttgca ccaggtcttc acgatgggtc gcttcgccta accgaacttg ctccagcaac
    21361 tgttgctgag cggttggtgc tgcctcgacc atcggcatga cggccagacc gagggaaagc
    21421 gtgaatatgt ttagtgtgaa tttgcgcatt cctggcccca gtcaggtaat aactcacctt
    21481 ttgtcgagaa gcggaaacgg tgttgatccc agccttgtcc aaacagggtc agcacatagt
    21541 tgtaataggc atcgctgcca ggaaagttat cggccacgcg ctggcgctga acggcctgcg
    21601 catcgcggtt ttgtaaaaag ggcagcatgg cggcagaaaa accgaccggt cctttaccct
    21661 gcgctttccc cgtagccaca tccacttttt ccggcggata accgtttttc tcagtgaatg
    21721 tcgccatcgg tttaaaccgg ttgagcatcc gcgctttttg cggatcgctg tcaggcatca
    21781 tgcctaccca catgtaaacg cggatagcgt cgtagctgct gatcaatgtt ttttcggctt
    21841 ttagctgcca gcctttgtct ttctcatagc gcacccagtc tggcgaaaag cctttcgggg
    21901 cggtttccag caataaacgt tgattggttt cgcgcagcgt ggtccacggc gcgccaaagc
    21961 gggtgaaata ctgcgccagc gtcggcggca ggtagctggg gttaaaacgc cagctgttat
    22021 cctctgcaaa tcccactttg cccggtaaca acatggagcc cagcccaggc accgtcacca
    22081 cttcctcccg cgcgatacgt tttagcaatg cgctgccgat gtcggtataa cgctgctctt
    22141 tccacaaacg ccccgcctcc agcaacgccc aggccatcca gacatcacca tcggaggccg
    22201 aattgctgtc cagcacttcc cacttactgt tctctttctt gccccacagc caggcgggca
    22261 aatgttcttt taaagaaccc tgtgcgagat tgttctgcgt ccagtcgaga agattatcga
    22321 aagctgcacg gtcgttagcc gccagggcaa agaacatgcc gtaactttgc ccttcggagg
    22381 tggtgatttt gcgcgcgtcg ctggggtcga tgacgcgccc ttcctgactg atgtaatcct
    22441 ttttaaactg ctcccaggca ggccaggtac aggctgcctg aacactaaag gcagccagca
    22501 gcagcatcgt cacgagtcca ctacgcaaca cattcatctt caattactcg ttatccgggt
    22561 taagacgacg acggctgata atacgcagca gacgccacag tacccatgcc agcagcacga
    22621 cgctgattgc cgccagcacc gccagcagaa tcggatggtt tgccagcgca taccataagc
    22681 gttcgaacca cggcagatgg ccaacgtaat agacgtcgcc aacacgcagg ctattgatac
    22741 cggactcgcg gatcaccgcg accgaaccga acatggtggc gcgtttgccg ctatcgttca
    22801 ccgcatcgtt aagcatttca taaccgcgtg ggctatctgc cagcagcgca atcacgctac
    22861 gctggtcgtt atacggcgac tggaagccaa tcaccgctgc catcgcacct gaagaggtca
    22921 gcgttgactg agtttctgcc gcgcgatcgc tctcgtccgg tacaatgccg gggaacgggg
    22981 tctggcgcat cggcgttttc acccagcttt cggtcgcctg caccaacagg tcgatctgct
    23041 tgtcgtcttt cagtttgtcc gggataccac cgacgatcat gatgtcggca tctttcccct
    23101 gaatagtgct gccatcatcg gtcaccgtca ggttaatcgc cgggaagccc gtctgcgcac
    23161 cgataaagcc aacggtattc agcaaggttt ccatctgtgc ttcgttaggc gctttcggca
    23221 tcacggtgat ggtttgtgac agatccgcca tccggctgaa cgggaagccc gcgttagcaa
    23281 aggcgcgtag atccggcatc gggatgaagt ggtaatactt cgagaagtcg atggtggaat
    23341 catcaccaat caccacatga ttctgcaccg gctggaaggt aatgcagtta tccaccgaac
    23401 cgcccggcat cgggttcata tactcaaagt caaagcgcag ctggttggtc gcgcccagtt
    23461 tcagcgccgg aatagagaca tccgttttgc catccagcaa accttgtaat accggaatac
    23521 gcagcagcag gcggttcgcc tcctgtttac tgctcaggtt gaaggattgc aggaactggt
    23581 tattcaggct gatatccatc cgtgagctgt ctttcactgg cggcatggtg tagcgataat
    23641 taatatccat atcaatgccg gtactgcgca tcaggtagag atccggtggc aggtttagcg
    23701 aaacgttaat cgctgctggc tcaagaccgc tggattgtaa ctgttcttca taggttttca
    23761 gttcaccaaa ggtaaccgga cgatcggtac gcacccagtt cggcgcatcg tacggcttac
    23821 gcggtagcag cggtttcact tcattcacta ccacgctttc accacggaac agaatgttac
    23881 cctgagcgat acctttcgct gcctgcaaca ggtctttgtc gtcacgacca aacaccacca
    23941 gcagtttgac ataaggattc tgcggatggt tgatcatttc aatcaccggg gcttttaccg
    24001 ccggatgatc gcgcaggaag tccgggcgtt tgtcattggt ggcaaaaacg atggcattgc
    24061 gatccggcaa ttggttatag agcaccggga agttctgccc acgccagcca gaacgcgaac
    24121 caaaccacga ggcgacaatg gcagatgctt gttgcagccc gacatccggc gcacccgcaa
    24181 agaccatcgg caaggtgtta gtacggttat cacgcgggtc aaagaatggc accgggaagt
    24241 gtgacaggtc attcttcaca ttcagggtct gataggtcag atccagtcca ctgctccgcc
    24301 caacatccag ccaaagcgtg gtgctggccg ggttttcgca cacgtcctga taatgaccga
    24361 caaactccag ccgcacacgg ttgaagtcgg taataaacag tgggttaatt ggcatttgtg
    24421 ccagcgtttt tttacccaac tgttctttgg tcactggcag cacgcccatc agttcatcat
    24481 tgagataaac ctttaactgc gactggacag gcagtaatga tggcgatggc gtgtattcga
    24541 ggttgagcat cgccttcgtc accacttcat cgctgcgcat accaaactca atgctgccgt
    24601 tcgggttaat gccacgtagc accatgctgc ccggcggcgg cgcaatttgc gcaaaggtca
    24661 gcttcacatc acgggaagga ccgttctgcg ccacgactgg cgcatctgcg ccctgcacgc
    24721 ccggcatcac ttgccccacc tgcggatttt gctccgcctg ggcggctaca gctggctcag
    24781 cattgatcag tggttgcgtt gctggcgtcg cctgcgtcat gaaagagggg aacgcactca
    24841 tccccagagc cactgcacaa atccagaata tttttctttt catcgcgtta tcatcattgt
    24901 tgagccaaag cctgatccga tggttgtgcc gtttcgctcc gctccgggcg gcgcggaata
    24961 aacgatacaa cccaggaaac cagagaagtc agcacacgga atatgccctt caccgaagaa
    25021 ggcgcaaatt ccgccagatg gcggtagcca cggaagccga gcttcagaat atccagcaga
    25081 ctttccagcg gcttatcttc cgggtagctg tcctgccaga gcgcccatgt atccgcacgg
    25141 gcaaacgtac actgcacaaa atcgatatgt tgctgggtag tgagcggcat taatttcagc
    25201 ccaacttcat tacccatcac acgcgccacc tgagccggga agacgtattc ctgctgaccg
    25261 cgtttaagca acagattcac tttctgccct tccagaatct gcgcctgacc gttgatcttg
    25321 atccccaaac caccgtcgga gaaatcctga acggtacacg agaagaggtg accatcttcg
    25381 cgggcaattg ctgcgggcat cgtcatctcc acgcggtgcg atcggcgtac ctgtttgctt
    25441 tctaccgata ctgcgactgc gccgccgaga ataatcaggt tgtagaatac ccacaccata
    25501 ctgacgacca cggtgagcat ctcggttggc ggaccataga agtagcgcca gatcccaacc
    25561 gctacgccca ccaggttgag cagaacaagg aagatgtagg gccgcgagat cacccagtcg
    25621 acgtactctt cttccaccag tccacctttg gcggtgacgt taaatttgcc tttgtgcggg
    25681 ttaatcagcg ccaccagcgt cggtggtgcg atataccacg ccagcactgt ttcgtagatt
    25741 tcactccaga aagagtggcg atatttgccc tggatcttcg agttagtcag gctggcatgg
    25801 atcatatgcg gtagcacgaa cagggcgatc atcaacgctg gcgcgtagat gatgtaggca
    25861 tgaaacagca ggaacgccag cggcgcagtc aggaagatca gccgtggaat gcccgacaag
    25921 aaatggaaca tggcgttgac atagcacagc cgctgagcaa acttcagccc tttaccggtg
    25981 agtgggttat cgagacggaa gatttgcacc atcccgcgcg cccagcgaat acgctgaccg
    26041 atatgcgccg agagactttc ggtcgccagc cccgctgcct gcggaatacg catatacgct
    26101 gaggtgtagc cacgacggtg cagacgcaga gaagtgtgcg catcttcggt taccgtttcg
    26161 acagcaatac cgccaatctc atccagcggc ttacggcgaa tcaccgcaca tgaaccgcag
    26221 aagaaagtgg catcccacat atcattgccg tcctgcacca gaccatagaa cagcgtgcct
    26281 tcgttcggcg ttttacgaaa acgccccagg ttgcgttcaa acggatccgg cgagaagaag
    26341 tggtgcggcg tctgcatcat cgccagctgt ttctctttca ggaaccagcc catggtcatt
    26401 tgcaggaacg atcgcgttgg tacgtggtcg cagtcgaata tcgacacgaa ctcgcctttg
    26461 gcatatttca gcgcattgtt gatgttgccc gctttcgcat gttcatgagt ggtacgggcg
    26521 atatatttca cccccacgtt ttgcgcaaac tggcgaaact cttccctgcc gccgtcatcg
    26581 aggatccaga tgttcagctt gtctttcggc cagtcgatac ccagcgaagc gtaaatggta
    26641 tttttcacca cgttgagatc ttcgttgtaa gtcgggacaa agatatccac cgacggccac
    26701 agcgacatat ctttcggcaa tggcaccggc tgacgattca gcggccatac gacctggaag
    26761 tagccgagca ccagcactat ccacgcgtac gtttcggcga acagcagaat aagcccgcac
    26821 accaggctga ccggatcgtc ccagtttaac gtcgaggtgt aacgccacca gatataacgg
    26881 caagaaacgg tcagcgacag cacaatcagc atcagcgccg agaagcgccc cggcatccgc
    26941 cgtacgatca gcgctacccc ccacagcagc atcaggaaga taaactgcgc tagcgggtta
    27001 aacggctgag taacgcagat taacgccaga atcagcgaga aggtgacgat gataccaagg
    27061 atcaaccgcc gcgccccggc actcaaatga ccgagctctt ttttctcatc aagatgctgt
    27121 gttttatggc taacgcgctc aggcagctcg ttcatccatt gatggtaacg tccacgaata
    27181 ttttgtagac ctgaaaatgc ccgcctgcgc ggtttcggcg tttctttgcg cgatgcaccg
    27241 atcagtaacc agcatgtttg aataagataa cggaccgggt ccagcggacg cggacgcgag
    27301 gcgttgatat gcggatacag gtttttatgt tctgcgcgaa tacgctgcca gcgcgggtgc
    27361 tccagcggaa taaaaatcca ggccaggatc atccagaaac agccgagcgt cgcgctgaaa
    27421 gccgacgcac cgtgacgacg ataatcgcga taacgcccaa taagccgtgc gttgaccggc
    27481 gggataagca accaccgggt caggatactc atgatgcact cccgactggc gttttcagcc
    27541 cggaataatt caacaggcac cagttcgcca gcgtcagtat ctcttcagcc gccagcgcat
    27601 cactgcgata ttcaccgact ggttgcttag ctgccagaca ttcagccatc gcttcatcac
    27661 gatgaatgag catcggcagc aatcggcgct ggctttgcaa ccaaagctgg taaatatcgt
    27721 cctgaacctg gctgccaata cggaagtcat taatcaaaat atgtgcacca tccggcagcg
    27781 cttgctgatg cagtcggata tggcagttgg catccacatt gacgattgcc agcgagtgat
    27841 cgcacaaact cagcagctgg tgggttatct gcgaggcatc acgcggtaag tcaattaaaa
    27901 tccactggta acgcccgctg gcttttagtt gctgtaagcc ggtgcaaata tcgctcagcc
    27961 gggtttgcca gtgctgtgga ttttcttgtt cttcaatcga taactgacca aaaggcagca
    28021 aatcgagctg cgaggtatag cgcaacccgg cgtcacgcca gtcctggcca tccagcatag
    28081 ctctggccca accctgacgg tgggtaaaat caacgttaaa tgacaggcgc aacaagttgt
    28141 ccgggcaggc atcgaccacc aggacatttt ctcccaacat ttgtaatgac caggctaatg
    28201 cggcggtgat ggtt
//
LOCUS       DAZKJR010000062        27911 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKJR010000062 DAZKJR010000000
VERSION     DAZKJR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.062"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            203..466
                     /gene="yhcN"
                     /locus_tag="ACHOWS_002622"
     CDS             203..466
                     /gene="yhcN"
                     /locus_tag="ACHOWS_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFQ0048174.1"
                     /translation="MKIKTTVTALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(521..793)
                     /gene="yhcO"
                     /locus_tag="ACHOWS_002623"
     CDS             complement(521..793)
                     /gene="yhcO"
                     /locus_tag="ACHOWS_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFQ0048175.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(885..2852)
                     /gene="aaeB"
                     /locus_tag="ACHOWS_002624"
     CDS             complement(885..2852)
                     /gene="aaeB"
                     /locus_tag="ACHOWS_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFQ0048176.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(2858..3790)
                     /gene="aaeA"
                     /locus_tag="ACHOWS_002625"
     CDS             complement(2858..3790)
                     /gene="aaeA"
                     /locus_tag="ACHOWS_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFQ0048177.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3798..4001)
                     /gene="aaeX"
                     /locus_tag="ACHOWS_002626"
     CDS             complement(3798..4001)
                     /gene="aaeX"
                     /locus_tag="ACHOWS_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFQ0048178.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4184..5113
                     /gene="aaeR"
                     /locus_tag="ACHOWS_002627"
     CDS             4184..5113
                     /gene="aaeR"
                     /locus_tag="ACHOWS_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFQ0048179.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5241..6686)
                     /gene="tldD"
                     /locus_tag="ACHOWS_002628"
     CDS             complement(5241..6686)
                     /gene="tldD"
                     /locus_tag="ACHOWS_002628"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFQ0048180.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6842..10642)
                     /gene="yhdP"
                     /locus_tag="ACHOWS_002629"
     CDS             complement(6842..10642)
                     /gene="yhdP"
                     /locus_tag="ACHOWS_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFQ0048181.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGQVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10710..12179)
                     /gene="rng"
                     /locus_tag="ACHOWS_002630"
     CDS             complement(10710..12179)
                     /gene="rng"
                     /locus_tag="ACHOWS_002630"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFQ0048182.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12169..12762)
                     /gene="yhdE"
                     /locus_tag="ACHOWS_002631"
     CDS             complement(12169..12762)
                     /gene="yhdE"
                     /locus_tag="ACHOWS_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFQ0048183.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12771..13259)
                     /gene="mreD"
                     /locus_tag="ACHOWS_002632"
     CDS             complement(12771..13259)
                     /gene="mreD"
                     /locus_tag="ACHOWS_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0048184.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13259..14362)
                     /gene="mreC"
                     /locus_tag="ACHOWS_002633"
     CDS             complement(13259..14362)
                     /gene="mreC"
                     /locus_tag="ACHOWS_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0048185.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14428..15471)
                     /gene="mreB"
                     /locus_tag="ACHOWS_002634"
     CDS             complement(14428..15471)
                     /gene="mreB"
                     /locus_tag="ACHOWS_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFQ0048186.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15776..17716)
                     /gene="csrD"
                     /locus_tag="ACHOWS_002635"
     CDS             complement(15776..17716)
                     /gene="csrD"
                     /locus_tag="ACHOWS_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFQ0048187.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17868..18842
                     /gene="yhdH"
                     /locus_tag="ACHOWS_002636"
     CDS             17868..18842
                     /gene="yhdH"
                     /locus_tag="ACHOWS_002636"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFQ0048188.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVTLLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            19028..19415
                     /locus_tag="ACHOWS_002637"
                     /pseudo
     CDS             19028..19415
                     /locus_tag="ACHOWS_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            19820..20290
                     /gene="accB"
                     /locus_tag="ACHOWS_002638"
     CDS             19820..20290
                     /gene="accB"
                     /locus_tag="ACHOWS_002638"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0048189.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20301..21650
                     /gene="accC"
                     /locus_tag="ACHOWS_002639"
     CDS             20301..21650
                     /gene="accC"
                     /locus_tag="ACHOWS_002639"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0048190.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            21759..22001
                     /gene="yhdT"
                     /locus_tag="ACHOWS_002640"
     CDS             21759..22001
                     /gene="yhdT"
                     /locus_tag="ACHOWS_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFQ0048191.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            21991..23442
                     /gene="panF"
                     /locus_tag="ACHOWS_002641"
     CDS             21991..23442
                     /gene="panF"
                     /locus_tag="ACHOWS_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFQ0048192.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRTVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            23454..24335
                     /gene="prmA"
                     /locus_tag="ACHOWS_002642"
     CDS             23454..24335
                     /gene="prmA"
                     /locus_tag="ACHOWS_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0048193.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            24664..25629
                     /gene="dusB"
                     /locus_tag="ACHOWS_002643"
     CDS             24664..25629
                     /gene="dusB"
                     /locus_tag="ACHOWS_002643"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0048194.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            25655..25951
                     /gene="fis"
                     /locus_tag="ACHOWS_002644"
     CDS             25655..25951
                     /gene="fis"
                     /locus_tag="ACHOWS_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFQ0048195.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            26037..26921
                     /gene="yhdJ"
                     /locus_tag="ACHOWS_002645"
     CDS             26037..26921
                     /gene="yhdJ"
                     /locus_tag="ACHOWS_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFQ0048196.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAVLVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVANGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            27005..27199
                     /gene="yhdU"
                     /locus_tag="ACHOWS_002646"
     CDS             27005..27199
                     /gene="yhdU"
                     /locus_tag="ACHOWS_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFQ0048197.1"
                     /translation="MIRKYWWLVVFAVVVFAVFVFLFDTLLMQWIELLATETDKCRNM
                     NSVNPLKLVNCDELNFQDRM"
     gene            complement(27202..27864)
                     /gene="acrS"
                     /locus_tag="ACHOWS_002647"
     CDS             complement(27202..27864)
                     /gene="acrS"
                     /locus_tag="ACHOWS_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFQ0048198.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT         6820 a         7397 c         6968 g         6726 t
ORIGIN      
        1 tctaattgaa aagaatagaa ttaataataa ataatcgtgt ttttaattct tttttgttat
       61 taaaactcac atttttaaca cttagtatca actgaaacag ttagcgtggt attaattagt
      121 tcaataatta gtgtatactt gattttgtga tatgggtcac gaaacaaagg cccagctaaa
      181 agattatgtc gaggtaaaaa tcatgaaaat caaaaccact gttactgcat taagcgtact
      241 ttctgttctc tctttcggtg cattcgctgc cgactccatt gatgctgcac aagcacaaaa
      301 tcgtgaagca atcgggaccg tatccgtaag tggtgtggcg tcttcgccaa tggatatgcg
      361 tgaaatgctg aacaaaaaag cggaagagaa aggcgcaacg gcctaccaga ttactgaagc
      421 tcgtagcggt gacacctggc acgctacggc tgaactgtac aaataaaccc tcatcgtctt
      481 gtccgacgat attgcccccg gttcgggggc tttttttgcg ctaatgacga acattaaaac
      541 gcaaatgccc ttccagctct tcctctgcct catcaaacag caatattaac gcgccaaaac
      601 ggcgacgcgt tttctctccc agatgaacaa attcaatctc aagtggtagc ggcaggacat
      661 cgttcattaa cacatcccat agtgagtcga gatcgcgtac cttatctttc gccagaccaa
      721 aggcttggct gaagtcgcga taaaaatcct cctgactctc aatctcatca aaatcaaagg
      781 tataaatatt catctgttgc caccgtcacg tttcacggcg agttctgccg ttcatcgcta
      841 agtatagcca tgaaaaaacc gacgcttcgg gcgtcggttt tgacttaact atcggtcaac
      901 gcatgttgat acttatggag catccccgcc agccgatgaa ccggttccgt cacctgcggt
      961 ggcgcttgcc agatgcgtag cttttcctgg tagatttcaa gttcttccag caactggcca
     1021 aagtaccgac gacgtttatc atcgctacgg gcagatatca catggtctgc tgtgcggcgc
     1081 atttgtcggt gaaacgccga taaatcctcg ttaaccggga tcggcgcatc acgcaggcgc
     1141 tggtgcgcga taatcatcgt cagcgccagg cgaaatttcg gcaaatcccc tgggaactta
     1201 ttcatcagca aaaacagctg ctgataaagt gccgggaggt gattctcttt acgacgtgcc
     1261 acattggtag tcatcgcgga aacagcggca gaaacaaact gattaagcag tacacgtcca
     1321 gtcctgtcgc gcgatttatc ccgcaccagc aaaataacgg tgaacgcgag cacacagccg
     1381 acgatttgcc ctaatgcgct gtcgagaaac tgactgaaat ggaaagtcat cgggttatcc
     1441 agcacgataa tatttatggt gctggccagc gcccccatcg agcccagtcg ccgtttctgt
     1501 acttctatac cgaggaaaaa tcccagcact gccaggctaa tacacagcaa taacatgctt
     1561 tgttgggtat tagggataat caccaaaaag tagagcagcc ctaacggcag cgccgccagc
     1621 gtcccataga taaagtcgat cgccaccatg cgtggattcg gcaaacgcat tgccagtgac
     1681 gtcacgaccg caatcatcac cattgcacca ctgccggaag tccagcccgt ccacagccag
     1741 aaaagcgtcc ccagaatgca ggaaagtgtg gttcgccaga agttaaccat cgcatgatga
     1801 cgttcggctg actctacttt gacttccggt tcgccttgca ggatctcttc ttcagtagcg
     1861 ttgatttttg tgttactgat aacgccgcgc ttgagaagct gataacgcgt tgccgccgcg
     1921 acccagctat aaatggtgac aggcgtttcc cgttccccgg tccaggcgat aactctccgc
     1981 aggcgtttga gctgcttgtg gacgtcctgc gcggtttcta ccggcgtgtc aaaaaattcg
     2041 cggaaagtat cagtgatcaa ttccgggcgc gtattctgaa taagataagt ttcgcaggat
     2101 tgggtaatca gcgtcagcga tagcgtattg atcgctttta aacgtcgatt ggcccgcgcc
     2161 cagcgggaag attccatatt caggttgctg cgcatacctt gtaacgccgt ggtgcgtcgc
     2221 accagatcgc cccaggcttt atcgacaact tcaccatcgc catgtttgat acagagttgc
     2281 attaattgat attgcgcgac cagcaaactt tccagctctc gatccacttc ttgtttgatc
     2341 gatcgcggag aaaagagcaa atccgccata atcgcacaca caataccgat aacgatctcg
     2401 ctacaacgtt cgacggcaaa ctgcggcgta agcaatggtt ccggctgaat ggtgatcaca
     2461 atgatcagcg cggtataacc ggccagcccc cacgcatacg agttttctat tcgtaccagc
     2521 gaggatatcc aggtacaaaa accggcccag atacagcaca ccagaatcat caataatggt
     2581 gcgcggatca tcgcaatgat gattaccagt ccagcaatac agccaataaa tgtgccgatg
     2641 atgcgcaaaa agccacgata gcgaatagcg ccagaatacg gttcacctcc cgcagcaaag
     2701 gccggaccgg cggcaacaat cgccgctgtc agtaccgccc agcgtggcgt ttccagctgg
     2761 aagtgaaagc caacaaacag cgccagtaca atggcggtcg ccagttttac cgcaaagcga
     2821 atatgttgat tagcaatgga gaaaataccc atcgtgatta accaaactca cgcaggcgat
     2881 gggccatttt acggaagaac gaatcctggc tttcgtcgcg atcttgtttg ccagtgacca
     2941 ccactgtagc agtggtgccc gcaggccaga tgttctcttg ctggttgtcg agacgaatac
     3001 gtaccggaac acgttgcgcc aaacgtaccc attcaaggtt agagtcgatg gtcgccatcc
     3061 ctttgtcgtc acgcgtgctg ctggcgttgg tgacccctgc ggcaacacta tcaacagtcc
     3121 ctttcagcac tttgttactg ccaagcggcg tgatctccgc acgataccca gggcgcaccc
     3181 cttccagctt agtttcttcc atataggcca gtacatagaa ggagttctgt ttcaccagcg
     3241 caaccgccgt tgatcctcga gtaataaact caccggtata gacgttgagg ttggtcaccc
     3301 agccatctgc tggcgcgcgg atgactgtac gttcaagatc cagttttgcc agatcacggg
     3361 tcgcctgcgc tttcgctaac tgatgcagaa ccgtttgtag tacgttgttg gcctgatcga
     3421 tctcttcgcg agacatcgcc tgcacaccga gacggttacg acgtcctgcc tcctggcgtt
     3481 tctcctgtgc cagtacctga taataagcaa catcggcttg cgcttcctca agcgcctttt
     3541 gatagcgcgg ctggtcgatg gtgaacagta cctgtccttt tttcaccagc tggttatcat
     3601 gaacattcac ctgggtaatg agtccagaaa cgtccggcgc gatcgcaacg acgtcagcgc
     3661 taaagcgcgc gtcacgcgtc cacggggatt cggtgtaata gacccaggca ttaaaaattg
     3721 cgatgaaggc cagaatgact aatacgaccg tgatggccgt acgggagaat tttcttatta
     3781 gtgttttcac ttcaacctca aacgaacagt cgcgatatca aataaaacaa gcagcaatag
     3841 agcgcggtgt tgaacaacgc cggatgccag acaaagtcgt agatacctgt tggcacaagt
     3901 acccggcgca ccagccagaa aatcgccagt gataaaagca attcaaaaaa tatcggtggg
     3961 aaggacagcc caaacaccac gataacggga aacagactca tgttgacctt ggttgtatag
     4021 agagagcagg cgttattatt ttcagcatct gtcgccgcag agaagggcat ggaaagccgg
     4081 gcgagagcaa cattgctgta gattgatatt taatatatta gcgtaactgt tatgctgtta
     4141 tctatattat gtgatctaaa tcacttttaa gtcagagtga ataatggaac gactaaaacg
     4201 catgtcggtg tttgccaaag tagttgaatt tggctctttt accgccgccg ccagacagct
     4261 acagatgagc gtttcgtcca tcagtcagac ggtgtcaaaa ctggaagatg agttacaggt
     4321 aaagctgtta aaccgtagta cacgcagcat tggcctgact gaagccggca gaatttacta
     4381 ccagggctgc cgtcggatgc tccatgaagt gcaggatgtt catgagcaac tgtatgcctt
     4441 caataacacc cccatcggga cgctgcgcat tggctgttct tcaactatgg cacaaaatgt
     4501 tctcgccggg ctgacagcaa aaatgctgaa agaataccca ggtttgagcg tcaatctggt
     4561 taccggaatt cctgcccccg acctgattgc cgatggtctg gatgtggtga tccgcgtcgg
     4621 cgcgttgcag gattccagcc tgttttcccg ccgtctgggc gcgatgccga tggtggtgtg
     4681 cgccgcgaaa agctatctca cacaatacgg cataccggaa aaacccgccg atttgagtag
     4741 tcattcatgg cttgaataca gcgtgcggcc cgacaatgaa tttgaactga tcgcaccgga
     4801 agggatctcg actcgcctga tcccacaagg aagatttgtg actaatgatc cgatgacgct
     4861 ggtgcgctgg ctgacggcgg gtgccgggat cgcctacgtg ccgctgatgt gggtgatcaa
     4921 cgagatcaat cgtggggaac tggagatcct gctgccgcgt tatcagtcag atccacgccc
     4981 ggtttatgcg ttatataccg aaaaagataa gctgccgctg aaggtacagg tcgtgatcaa
     5041 ctcgctgacg gattattttg ttgaggtcgg taaattgttt caggagatgc acgggcgcgg
     5101 gaaagagaag taattcatat tgtactgtta cgttgtacaa acctgtgcca acgggttccc
     5161 ctcaccctaa ccccagagga gcgaggggac cgttcgtgca cgtagaaaga ttaattatcc
     5221 ttctgaaaat actgaaatta ttacgcagta ccgccaacag tcaggttatc gactttcaac
     5281 gttggctggc ccacgccaac cggcaaactt tgcccttctt taccgcagac acccacgccg
     5341 ttatccagtt tcaggtcgtt acccaccatc gaaatctgct gcatggtttc gataccggaa
     5401 ccaatcaacg ttgcgccttt caccggcttc gttactttac cgttttcaat cagatatgct
     5461 tctgaagtgg agaaaacgaa tttgccggag gtgatatcca cctgaccgcc accaaagttc
     5521 ggtgcataga taccgtactc aacggattca ataatttcct gcggggtcga tttacccggc
     5581 agcatatagg tgttggtcat acgcggcatg ggcagatggg cgtaggattc acggcgaccg
     5641 ttgccagtcg gcgtcatccc catcaaacgc gcgttgagtt tatcctgcat gtagcctttc
     5701 aggatgccat tctcaatcag cacgttgtac tggcctggcg taccttcgtc atcaatcgcc
     5761 accgaaccac ggcgatcgac catcgtaccg tcatcaacca cggtacacag ttctgaagcc
     5821 accagctccc caacctgtcc actaaatact gaagtgccac ggcggttgaa gtcgccttcc
     5881 agaccgtgac ctaccgcttc atgcaacagc acgcccggcc aacctgcgcc aagtactacc
     5941 ggcatggtgc ccgctggtgc agcaacggca gaaagattga ccagcgccat acgcactgct
     6001 tcttttgccc atgcatccgc acggacttcg ccgtcgagat cggcaaggaa gaactcataa
     6061 ccaaaacgac cgccgccgcc actggcaccg cgttcgcgtt tgccatcttc ttcgaccaga
     6121 acgctcacgg aaagacgcac cagcggacgg acatccgccg ctagcgtgcc gtcggtggcc
     6181 gcaaccagaa ttaattcata gacaccactg aggctggcag tcacttcctg tacgcgcttg
     6241 tccgcttcgc gggcaacctt atcgacgcga cgcaggatat ccagcttctc ttcacggctc
     6301 atgctttgca gcggatctac cgaggtatac aacgggctat gctctaccgc gcccagcgtc
     6361 tgtactttac catcaccact atcacggacg atggtgcgcg ccgcttgtgc actctgttcc
     6421 agcgccagca ggctgatttg gtcagcataa gcaaatccgg ttttttcacc actgattgca
     6481 cgcacgccaa cgccctgatc gatgttgtaa gaaccatctt taataatacg gtcttctaaa
     6541 acccaggatt cgtgatagct cgactgaaaa tagagatcgc cataatcaag gcgacgttcg
     6601 gccagttgac cgaggatcgc gaacaagtcc tgatgtttca ggccgttcgc cgctagcaat
     6661 tgttcactta ccaggttcag actcatcgtt tttgctactc gttagttact gcagtagagg
     6721 attttttacg gctgccggat gcggcgtgaa cgccctgtcc ggcctacggt tatgttctgg
     6781 ctcgttggcc tttggcaacg attatcctat gagattgggg caattacgcg ccctcgtcaa
     6841 atcattgcgc tttttcttta cgcggttgtc gcaacacttc gttgatttgc ggatcgtcca
     6901 gcggacccga aatgtgatag cgcaaaatgg agactttgct ccacagcggt cccagcactt
     6961 tactggcggc aaacactgcc gcgccaacaa tggggttaac cgcaaaggcc gcagccacac
     7021 ccaccgtcgc agaaatctct ggtgcgacaa ccgcttccat attcaggtcg cgacgtacca
     7081 gatttaccga ccctttcata gcgatgtccg cttccaggcc atccaccagc gtgtcgtcgg
     7141 tgtgcataac gccgtcttta atccacgcgg tgctgcgaat ggagtcaaaa tagaaccctt
     7201 cgccaaaagt gtctctgaaa tcaaaacgca gcttacgcat cagggcatct acgctcaata
     7261 agcgcagcaa ttgcccggca tgtccggtat tgatttcggt aatttcgcct ttacccagtt
     7321 gagtatgaat gatgccattc aacgtcgcct catctggttg ccagggtgct ttgcgccagt
     7381 gtaaatcgta atccacatta aatgacgact ggcgtatggg cgtcgtgaca ccaaaaaatt
     7441 ctgcggcggc atcaattttc tgcccacgca gttttccttt cagcgaggtg cgttcattcc
     7501 ccggattgtt aacccattca ccatcggcag tgagccgcga aaaaccagta tcaatcagtc
     7561 cattagtcag cgttaatgta ttgccagaaa tggtgatatc actgtcaatg cgcccgaatt
     7621 tttgccccca gaaccagcac tctgcgcatc gtatttgtgc gtccggccag ccacggaagc
     7681 taatgcgctc cgttgtcggg aaaggtgatg aattcgttga gtcaccatga gttttcgcca
     7741 cgcttgggtt gtaataaaga tatttgatat tcgccagcca cggcgcgtta ttacgcatcg
     7801 ccagcgtggc gttgatttca cgcccttgcg cctcaaccag ggtgccattt gccgtcggtt
     7861 gcgaaacaat gctcaggtta ttccattgct gatttcccag cgacaacata ggcgtacgta
     7921 acgttatgtg ttgtgggaaa ctcgctgcac caccgacact ctccgcagcg cctttctgga
     7981 acagggccag ccactcggca ccattcatcg gcggcatatt gagttcaacg ccactttgtt
     8041 ccggcaacgg cgggagcgtt ttactgtctg ccgcccaaat agcacggtcg agcgtcagct
     8101 tttgaccgag caaccagcgg ctattgaaat ggttatctgc accagcctgt ccggttaact
     8161 caaagctgtt gagattgcca tcaaccttaa cgtttaccgg cagtggttca cccgcaggtt
     8221 tggctaacgg tgaaggtaag tgactgctca cattcttcag gtcgccgttc agctctacgt
     8281 tataggtcgc gccagcatga taaggcagat caatgcccac tttaccatcc cacgccacgc
     8341 tgccacttaa tgcttcgttc accgcttcag gcagaacgcc ggttttcgct ggttgccagt
     8401 taccgttgag attcaccgcg acctggtagg cttttgcccc ttctttggtg gaaaaatcca
     8461 cgttcaacgg ctgattaaac cagcttgctg tcagtggttc actttgcaga tcgccattga
     8521 taaagctgaa tttaccgctc aaatttttca gggtgctgtc gagtggtttg ataaacagac
     8581 tgttattacg cagcgtcaca tcacctttcg cggttaccag ttcgccgttc agcgggatat
     8641 caagatgtaa gcgagcattc acatcgccgt cgagctggag ttcttgcagg gtcgcaccca
     8701 gagaatcttt cagcggtgtc tcatcaaagt aagggccaac ggctttaccc ggacctttaa
     8761 tgtcagcgtc aatcagcagt ttttcttttg agtagtcagg gatcactgcg gtgagattac
     8821 ttgcgcgcac gccgcccaga ttaacgccat cggttttcat ccataaacca tcgttaataa
     8881 agtccagttc aatatcaagg ttagttaatg caggccagtc cggctggaag gcaaacttcg
     8941 cgttgcgcag cggtaccagc acttcaaact gaccttcgtt gtgtttatag gggaagagtt
     9001 gcggattgcc accataaacc agcgtcgcgt tatccgcttc accgccctga atcgcgccac
     9061 ttaagtaatc aaccaggtct ttacccatca agttttccgg gaaatagcgc caggcttgtg
     9121 aaccatcatc ggtactgatg ccagccagaa tacccagcca gggttcatcg ttagctggtt
     9181 gcaggtaacg aaaaccgccg cgcgcatgga cggctttggc tttaacgtca atattacgcc
     9241 catccagctg gaaacctttg tcattgttca gccagcttat cgttgcctgg ccgtcggcga
     9301 tttccagtgg cgcacggaat accgtttcgt aaggcatctt tgcctgcttc atcgacgcag
     9361 taagcaaacc attttctacg ctgccggaaa gcgtcccgga gaagtgttcc gcacccggta
     9421 ataatttcca ttgcttccag gccagatcgc tccacgatgc ctgaaaacgg gtcttgtctg
     9481 ccgcctgaag cgggatatcc agcgccagcg tgttaatctt gccgctcggt tgtgtggagc
     9541 gccaaacatc acccagtgca ggtgaaagtt tcgcggccag cgggcgtatg ccctccaggc
     9601 ctgccagctc cagattactg gcgcgaatcc ggagttcgtc actgcgttta ttgtctttgc
     9661 cgccaacgtc ctgttccggt atccaggcca gcgtcaatgc tccgctcggc cagggtttgc
     9721 cgtccatcgt gatccgtgta tcgggaatag agaactgcca gcctggattt tcacgcgtaa
     9781 tatgcgcggt cagattatcc accgacagcg tatgcgtttg cttctcgccc aaccagctgg
     9841 caccgccctg tttcagccag acgtcaccgc cggttacatc gcctttgtcg atcgtcatcc
     9901 agccttcaag ggagaactgt gccgtttcca gcgcaatatt gtcctgcatc catttaccga
     9961 gccacggctt caggtcgatg tcatccgcct ggagccagac gcgaccattg cttaacaacc
    10021 cctcatcatc gcgcaaatcc atgcgcacct gcatcacgcc gtgctgtccg gtaaggctgg
    10081 agaggcttac ctggccttct gcacggtgtc gacgtggatc gttcagccag gtgagctgtg
    10141 ggatcgccag ctcggcgcgc tgaccggatg gcgtcaggaa actgacttca ctgtcgcgga
    10201 gatcgaaatg gtcgaactga cgaagaaaca gatcgctgat gtgactggct tccagactat
    10261 cattaccacc gccgctggtg ataggagtgt tggtgcgaaa gcgcagctgc cagaaagtga
    10321 ggtcgcgaaa ctgccagcgc atatgtaaca ggctctgcca gacatccagc gccagagtaa
    10381 cgcgtttaac cgaaaattcg ccgccatctt ttagttctgc acggatgtcg tgcgcttcaa
    10441 gcgtcggacc aaaattctgc cagctggctg agagctgact ggcttctacc ggcatgccag
    10501 tcgcggattc tattttgttg aggatttccg gacgccaggc gtcaagatgc ggtaaagcaa
    10561 tacgcaggcc gctaaccagc agggcagcga tcacaacgag cgcggctcca gtaagcagta
    10621 aaatccccgg caatcgcctc acccgtcact ccttgtctgc taaaaatgtg actcaaaaac
    10681 cctttgccgg atggcggccc agcatctgtt tacatcatta cgacgtcaaa ctgctcctgg
    10741 ttatagagcg gttcaatttg tactttaacc tgtttaccaa cgaaaatttc cacttccgcc
    10801 agcgagtgtg actcttcgcc tttcaaggct tcagctactg ccggagaagc atagaccagg
    10861 aaacggtcgg aatcataagc atggtgtaca cgaacaatct cgcgcatgat ttcatagcat
    10921 accgtttcca cggttttcac cgttccgcga ccgtggcagg ttgggcattc gttacatagt
    10981 acgtgctcaa tgctttcgcg ggtgcgttta cgcgtcatct ccaccagccc cagcgccgaa
    11041 aaaccattaa cgctggtttt cacccggtct ttgctcaacg cctgctccag cgagtgcagc
    11101 acgcggcggc ggtgatcttc attattcata tcgatgaaat caataatgat aatcccgccc
    11161 agattacgca accgtaactg gcgagcgata gcctgcgtcg cttcaatatt ggtattgaaa
    11221 atggtgtcgt ccagattgcg atgaccgaca aacgccccgg tattgatgtc tacggtggtc
    11281 atcgcttcgg tctggtcgat aatcagatag ccaccggatt tcagttctac tttgcgttcc
    11341 agcgctcgct gaatttcgtt ttcgacatca aagagatcga aaatcggctg gcgtccggtg
    11401 taatgctcca gcttgctggt catctcggga atgtactccg aggtaaactc cagcaatgct
    11461 tcgtaagtca ggcgtgagtc aacgcgaatg cggtcgagtt cggcgtcggc gaaatcacgc
    11521 agaacacgct gcgccagcgc cagttcgccg tacagctgat aacgggtctg tgggcgcttt
    11581 ttacgctcca taactttggt ccagacgcgt ttcagataag cggcatcgga ggccagttca
    11641 gcctcgccaa ccccttcagc tgcggtacgg atgataaacc cgccctgctc gtcgcaatac
    11701 tctgcgacca cttttttcag gcgttcacgt tctgattcgc tttcaattcg ttgggaaacc
    11761 ccaacgtgag aagcccctgg cataaatacc agatagcgag aaggcagcgt gatatcggtg
    11821 gtcaggcgcg cacccttagt gccaagtgga tctttcacca cctgcaccat cagatcttgc
    11881 ccctgacgaa ccagttccga gatgtcgcgc accgtgaatt gcttttgttc ttcacccgcc
    11941 acacattcgg tgtgcggcat gatgtcagat gcatgaagaa acgcggcttt atccagccca
    12001 atatctacaa aagccgcctg catacccgga agtacacgac ttacacgacc cttgtagata
    12061 ttgcctacta ttccgcgtcg cgcctcacgt tcaatatgaa tttcctgcag aataccgcca
    12121 tcaatatacg ccactcgcgt ttccgaaggc gttacgttta ctaacaattc agccgtcatg
    12181 tttatccctt ttctcacgca gtgcgttaaa attacttaat aattcatacg tttcaaccag
    12241 cggtaagccg actacggcgt gatagctgcc atttatcttc ctgacaaaac agccacccag
    12301 cccctgaata ccgtatgcac ctgctttatc taacggttca ccgctggcga catagcccgc
    12361 gatgtcttcg tctgttaacg ttctgaaagt cacatcggtg accaccaggc aatcgagaat
    12421 gtgctggctg tcggccaacg ccactgccgt cattacctga tgggtctgac ccgataattt
    12481 gcgcaacatc tgcgccgcat gctctgcgtc gcgcggtttc tccagtactt ctccgttcag
    12541 gataacgata gtatccgcac ccagcaccgg gagatcctgc gccgtttgcg cgacacccgc
    12601 ctgtgctttc tcgcgcgcca gacgcacaac atactgctgc gcgctctcct gcggctgacg
    12661 ctgctcctca atgcccgtaa caatacgttc aaaggttacg ccaagttgcg caagtaactc
    12721 ctgacgacgc ggagaaccgg aagctaaata cagagaagtc atagaaacct ttattgcact
    12781 gcaaactgct gacggacttt acgcatcagc aagaaaatcc acggccagag caccccattg
    12841 actacgctac tccagaacac ttccggtctg aaagagacgt taatcactaa aaactctgcc
    12901 cagaaaacaa taatatccac caccagcgaa agcaacatga cgaccagcgc ctgctgccat
    12961 aatgcgaggt tgcggaaaag ctggtatttc agcgccacca ggtaagcaat gatgctcatc
    13021 gccaatacgc gtacgccaag cgtcgagccg ctgatcagat ccagtatggc acccatcaca
    13081 aaacctgtgc ccacatttac gcgatgaggc aaggccagga tccaatacaa caagatgagt
    13141 aacacccagt ttggccggaa aacaatcagg ttatccggcc agggcatgat ttgcagcaac
    13201 agcgcaatga ggaaagagag ccagattacc cagcgtccct ggctacgata gctcgccact
    13261 attgccctcc cggcgcacgc gcaggcggtt gagcaccact ttgcggcggc gtagcccttt
    13321 gtggagagcg attagcagcg ggctgtgtgg gcgcagcagg cgcagtaact gcatttcctg
    13381 tcgccggttg ctgcggagtc ggctgagtga tccccgttgc cggttcaggt aactttggcc
    13441 ccatcgcgtc tggcgaaggc aatacctgcg gcatcatctg catcaaacgt tcattagcaa
    13501 cacgatgcac ctcttccggc gtcatcgggt tagcgccgtt acgatccgcg ccccacagca
    13561 gcagcagata acgcaaacgt tgcagtcctg cagtcggacg cgcctgaatc acagtataag
    13621 cgcgctgggt atcgagtttt acggaagaga caaccgcgac cggatagcct tccgggaaac
    13681 gaccgcccag accggaagtc accagcacat caccaacacg aatgtccgta ttcgctggca
    13741 gatgctcaag ctgcaaatca tccgtacaac cgttaccggc tgcaattacg cggatatcgt
    13801 tgcgcagcac ctggattggc agcgcgtggg tcgcatcaca aatcagcagc acgcggctgg
    13861 tcagtttagc gacggccacc acctgaccaa caacaccttt gtcgctgata accggctggc
    13921 cttcataaac gccattaacg ctacctttat cgataacaac ttgatcgcta taaggatcgt
    13981 taaccgtgga gataacctga gtcaccattt tctgctcatc ctgacgcagc ggggaaccca
    14041 gtagctcgcg cagacgcgcg ttctcctgtt tgtattgtcc aagcatcagc agttcactgt
    14101 ttttcagcaa cagttcctga cgtaacgccc ggttttcaag ttctagttgg tcacgcgaag
    14161 ccagcgtctg cgatacgcca tccagcaatt cacgaggagc attggaaaca aagtagaaag
    14221 gactgacggc ggtatccata taagtacgga tttgactgaa cgtccccagg cggctgtcgg
    14281 caataataat gccgagcgcc accagcaccg ccagaataag gcgaatctgt agcgacgggc
    14341 cacggctaaa aattggcttc ataagttatg cgtattctcg tatcagacca ggcagggtaa
    14401 acagacactt cccctgcctg catccgatta ctcttcgctg aacaggtcgc cgccgtgcat
    14461 gtcgatcatt tccagcgctt tgccgccacc gcgcgccaca caggtcagcg ggtcttcagc
    14521 aacaacgact ggaatgccgg tttcttccat taacaaacgg tcaaggttac gcagcagtgc
    14581 gccaccaccg gtaagcacca tgccacgctc ggagatgtcg gaagccagtt ccggcgggca
    14641 ctgttccagt gcaaccatta ccgcgctcac aataccggtc agcggttcct gcagtgcttc
    14701 gaggatttcg ttggagttca gggtaaagcc gcgtggaaca ccttctgcaa ggttacggcc
    14761 acgaacttcg atttcacgta cttcatcgcc cgggtaagcc gaaccgattt cgtgcttgat
    14821 acgttctgcg gtggcttcac cgatcagaga accgtaatta cgacgtacat agttgatgat
    14881 agcttcgtcg aaacggtcac caccaatgcg cacggaagag gagtaaacca cgccgttcaa
    14941 ggagataaca gcaacttcag tagtaccacc accgatatca accaccatag aaccggtcgc
    15001 ttcagaaacc ggcaggccag caccaattgc ggcagccatc ggttcttcaa tcaggaagac
    15061 ttcacgggca ccagcgccct gcgcggattc acgaattgcg cggcgttcaa cctgggtcgc
    15121 gccaaccggc acacaaacca gaacgcgcgg gcttggacgc ataaagctgt tgctgtgcac
    15181 ttgtttgatg aagtgctgga gcattttttc agtcacgaag aagtcggcga taacgccgtc
    15241 tttcattggg cgaatagcag caatattgcc cggcgtacgg cccagcatct gcttcgcgtc
    15301 atgacctact gcagctacgc ttttcggtga accggcacga tcctgacgaa tggccaccac
    15361 ggaaggctca ttcaatacga tgccttgtcc ttttacataa atgagggtat tcgcagtacc
    15421 caggtcaatg gacaagtcat tggaaaacat gccacgaaat tttttcaaca tactaaggga
    15481 taatcctgaa agctggggcg gaaaagaaaa tccgcttact ttaccaacca cacgcagcag
    15541 cgacaaggcg caaaaatcat ctgctacggt gaaaattagt gcagttcgtt tcctttgtta
    15601 caaatctctg cctgagtcca gaaaggctta atgcatcagc agcattcctc gcctgtttgc
    15661 aaccgcgtaa ggtcattcat ctgcatatgt gctgcaacaa tctggcgagc agacaagcac
    15721 actcccatga gacgcagcgc gcgttattct acgtgaaaac ggattaaacg gcaggttaaa
    15781 ccgagtatct ttgtgaatat tttttcacgt tagtatcaag tggctgtgag gacgcgaaaa
    15841 aatccccttg cccacctgta acaccgcgct gaatcagggt ctgccactcg cttcgcgaac
    15901 gcacgccggt ggcgtaaacc tgggtgctgg tcccggagca ggcttccacc aggctttgaa
    15961 ccagcagctg attctccgtt cgcttctcaa tgtttctgac cagccccgga tggagcttga
    16021 gtaactcaac attaagttct ttgatccaac tggtgcttac cagcgtcaaa ccagcctggt
    16081 tgacggctac ccgtacccct aaagcattca ctaaacgaat aacaggttgt aagcgactga
    16141 tatgttgacc aacatcggcc tctgcaagtt caataattat gcgtcttcgt tgcgattttt
    16201 cacattgcat taacgtatcg cgcagccaac gctgaaaacg cgggcgaatc agcgactcaa
    16261 cggtaacctg aatcgccaga ttttcctctg gccagtaacg caatagtgga ataagacggc
    16321 tgattagcag gcggtcatac tcttccgata aaccaaactg caagaccatc ggcatatact
    16381 ctgccgagct aacctcttca ttaccatcga agatgcggca catgagttca cgatgatgaa
    16441 cccggccttc gcgagtaacc gccggttttt gataaaggcg cgggccgccg cgactgagca
    16501 tttgctcgat aagcgtacgc cagcgaacat taccgcgtcc tttttcaggc aacgagtcat
    16561 cgtaaatagc ccagctattg ccgccctgca atcccgcatt acgcgtggca gactctgcat
    16621 gttccattac ctgctcggta tcctgaccac tacgccaggc acagatacca atgtggatca
    16681 tatcgtcgcg atcgagcatt ttattgttcg gcaaggtatc gacggctttg attaactgac
    16741 tggcgatgct ctctgcctct tttaacgtcc ggtgcggtaa cagcgcagca aaatcactgc
    16801 ggtggtaacg cgccagcagt gcgccagggt agcgcatcat aaatgtcgat agcagattcg
    16861 tcagagagaa gaactgttct tcaacctggc tgtgccccca ggtatcactc aacatattga
    16921 aatccggcag acgaatcatc atcacgatcc cgtgggtacc tactttctct tgatcttcca
    16981 gtaacgttgc taactgatta tcgaaaaaga gtcggttatt gaggccggtt ttcatgtcct
    17041 gggcggcata agagcggatc agcgtatcaa gacggctgtg ttgttcgcgt gcgttctgaa
    17101 tttcacaaag cagcgtatcc agcgcactgc tggttctggg cggccattca tagatggttc
    17161 ccaacacatt agagccacgc tcaccgttta agatacgagt agcccgggtt tccagcaatt
    17221 cttgcccggc aagttgccgt tgtaaccagc gtaccgccag gaagagcata agaatgataa
    17281 agccaatcgc ccccgtgagc ggcgcggtag tcattaaaga atggaaatag ttgcccatcg
    17341 gatcctgata aaccagacgc agcgacatcc ccggatgctt tatcaacgga acgctcagtt
    17401 cacgaaacag atcgttggtg ccaaccggac gataactacc atttctggcc agggtataaa
    17461 cctgcttatc accatggagc aggtctacac gaacgatatc tgccgacatc attaattcgg
    17521 taatttgtgg ccttaatgtg ctgaagtcat tcgacactag gtgggtatca atcgccgtcg
    17581 ccaccgcctg aacacgatga ctaaacttat actgaatggc gttgtagaaa cttagcgaac
    17641 agcccagcaa agtcacaaaa attgttaacc cggtgagcag cgtgacaaag gccgaaaatt
    17701 tcgtcgttaa tctcatcctt gtgttaactc cgatagtgag gaagcgggca tactagcaaa
    17761 tcagatttat ctcgcaattt attgcgcttc atcggctttg cttttccatt agcgagtata
    17821 gtcttcagaa attattttcc aatccatcat gcacatgagg accacttatg caggcgttac
    17881 ttttagaaca gcaggacggc aaaactctcg catcagtaca gactctggac gaaagtcgcc
    17941 tgccggaagg cgatgtcacg gttgatgttc actggtcgag cctgaactac aaagatgcgc
    18001 tggcgattac cggtaaggga aaaatcatcc gaaattttcc gatgattcct gggattgatt
    18061 ttgccggaac tgtacgcacc agcgaagatc cgcgttttca tgccggtcag gaggtgttac
    18121 tcactggctg gggcgttggt gaaaaccact ggggtgggct ggcggaacag gcgcgagtga
    18181 aaggtgactg gctggttgcc atgccgcaag ggctggacgc gcgtaaagca atgattatcg
    18241 gtactgccgg ttttaccgcc atgctgtgtg tgatggcgct ggaagatgcc ggtgttcgcc
    18301 cgcaggatgg ggaggttgtc gtgacgggtg ccagtggtgg cgtcggcagt accgccgtca
    18361 cgctgctgca taagttgggt tatcaggtcg ttgccgtttc cggtcgcgaa agtacccatg
    18421 aatatctgaa aagtttaggt gccagccgta ttcttcctcg cgatgagttt gctgaatccc
    18481 gtcctctgga aaaacaagtc tgggctgggg caattgacac cgttggcgac aaagtgctgg
    18541 caaaagtgct ggcgcaaatg aattacggcg gctgcgtggc ggcctgtggt ctggcgggtg
    18601 gttttactct gccaaccacg gtcatgccat ttattctgcg taatgtccgt ttgcaagggg
    18661 tggattcagt aatgacgcca ccagcacgcc gcgcacaagc ctggcagcga ctggtcgccg
    18721 atttaccgga atcattctat acccaagcgg caaaagagat atctctggca gaggcaccga
    18781 agtttgccga ggccatcatt aataaccaga tccagggtcg cacgctggtg aaggttaact
    18841 aaccatttag cagggaataa taagagaggg aactcatttt gaaatcatta ttcaatcggt
    18901 taacgggaaa agcggttagc cggacagctt tcgtcgaaca tcttggtcat gaagttgtac
    18961 aacatcatcc aaactggaaa gtcatgattt cgactgacca taaattgatg cgcattgatg
    19021 ctccaccaaa cagccattat tgatacgcct ccgtcgcctg ttaggtttat ggtgttttgc
    19081 cttggcgacg ctacgcttaa tctgttactg atttctggta ccatgggggg aataatctga
    19141 ttttatttga ttgcaaatta accacttaaa cctattgaat gcggaacatt gtcagtcggt
    19201 taattttgcg tgctttagca ttcacatctt tccggacgat gcagtgaaaa ttgcgtaatc
    19261 ctcagcaacc gctgcgtaat gtcgtctatc ttgtcgcgat cctggcatcc ctacattatt
    19321 tgtggtctgt gaagattatc tcattgcagc ccctcatctt cgcagggctg gctttacagc
    19381 ttttcaccct acgttataag aagttccgtc gatgatggcg ctaatttcgt gaattgtgcg
    19441 gcttgttgca aattacacgg tgttgaaggt tatttacatg ttagctgttg attatcttcc
    19501 ctgataagac cagtatttag ctgccaattg ctacgaaatc gttataatgt gcgacctcgt
    19561 cctccctgac gcagtttttg cgctgcggaa aaggtgacat tggcgcaacg aaggtatatt
    19621 ttgttttttg ccggaggata gcagcagatc gctgcacaat gtccgtcaag tctaacattg
    19681 acactctggg gcaaaataga ccggcgtccc ggcctgctgg aatttatcgc tatgcataca
    19741 gctgtcgggg catacgcttt acagacggcg gtgaaacgcc tgtcacaatc acactaaaca
    19801 aagagtacgg aacccactca tggatattcg taagattaaa aaactgatcg agctggttga
    19861 agaatcaggc atctccgaac tggaaatttc tgaaggcgaa gagtcagtac gcattagccg
    19921 tgcagctcct gccgcaagtt tccctgtgat gcaacaagct tacgctgcac caatgatgca
    19981 gcagccagct caatctaacg cagccgctcc ggcgaccgtt ccttccatgg aagcgccagc
    20041 agcagcggaa atcagtggtc acatcgtacg ttccccgatg gttggtactt tctaccgcac
    20101 cccaagcccg gacgcaaaag cgttcatcga agtgggtcag aaagtcaacg tgggcgatac
    20161 cctgtgcatc gttgaagcca tgaaaatgat gaaccagatc gaagcggaca aatccggtac
    20221 cgtgaaagca attctggtcg aaagtggaca accggtagaa tttgacgagc cgctggtcgt
    20281 catcgagtaa cgaggcgaac atgctggata aaattgttat tgccaaccgc ggcgagattg
    20341 cattgcgtat tcttcgtgcc tgtaaagaac tgggcatcaa gactgtcgct gtgcactcca
    20401 gcgcggatcg cgatctaaaa cacgtattac tggcagatga aacggtctgt atcggccctg
    20461 ctccgtcagt aaaaagttat ctgaacatcc cggcaatcat cagcgccgct gaaatcaccg
    20521 gcgcagtagc aatccatccg ggttacggct tcctctccga gaacgccaac tttgccgagc
    20581 aggttgaacg ctccggcttt atcttcatcg gcccgaaagc agaaaccatt cgcctgatgg
    20641 gcgacaaagt atccgcaatc gcggcgatga aaaaagcggg cgttccttgc gtaccgggtt
    20701 ctgacggccc gctgggcgac gatatggata aaaaccgcgc cattgctaaa cgcattggtt
    20761 atccggtaat tatcaaagcc tccggcggcg gcggcggtcg cggtatgcgc gtagtgcgcg
    20821 gcgacgctga actggcacaa tccatctcca tgacccgtgc ggaagcgaaa gctgctttca
    20881 gcaacgatat ggtttacatg gagaaatacc tggaaaatcc tcgccacgtc gagattcagg
    20941 tactggctga cggtcagggc aacgctatct atctggcgga acgtgactgc tccatgcagc
    21001 gccgccacca gaaagtggtc gaagaagcac cagcaccggg cattaccccg gaactgcgtc
    21061 gctacatcgg cgaacgttgc gctaaagcat gtgttgatat cggctatcgc ggtgcgggta
    21121 ctttcgagtt cctgttcgaa aacggcgagt tctatttcat cgaaatgaac acccgtattc
    21181 aggtagaaca cccggttact gaaatgatca ccggcgttga cctgatcaaa gaacagctgc
    21241 gtatcgctgc cggtcaaccg ctgtcgatca agcaagaaga agttcacgtt cgcggccatg
    21301 cggtggaatg ccgtatcaac gccgaagatc cgaacacctt cctgccaagc ccgggcaaaa
    21361 tcacccgttt ccacgcacca ggcggttttg gcgtgcgttg ggagtctcat atctacgcgg
    21421 gctacaccgt accgccgtac tatgactcaa tgatcggtaa gctgatttgc tacggtgaaa
    21481 accgtgacgt ggcgattgcc cgcatgaaga atgcgctgca ggagctgatc atcgacggta
    21541 tcaaaaccaa cgttgatctg cagatccgca tcatgaatga cgagaacttc cagcatggtg
    21601 gcactaacat ccactatctg gagaaaaaac tcggtcttca ggaaaaataa gactgcaaaa
    21661 gcgtcaaaag gccggatttt ccggcctttt ttattactgg ggatcgacaa cccccataag
    21721 gtacaatccc cgctttcttc acccatcagg gacaaaaaat ggacactcgt tttgttcagg
    21781 cccataaaga ggcgcgctgg gcgctggggc tgaccctttt gtatctggcg gtatggttag
    21841 tagccgctta cttacctggc gttgcccccg gttttaccgg ttttccgcgc tggtttgaga
    21901 tggcctgcat cctgacgccg ctgctgttta ttggactgtg ctgggcgatg gtgaaattta
    21961 tctatcgcga tatcccactg gaggatgacg atgcagcttg aagtaattct accgctggtc
    22021 gcctatctgg tggtggtgtt cggtatctct gtttatgcga tgcgtaaacg gagcactggc
    22081 accttcctta atgagtattt cctcggcagc cgctctatgg gcggtattgt gctggcgatg
    22141 acgctcaccg cgacctatat cagtgccagt tcgtttatcg gcgggcctgg agctgcttat
    22201 aaatacggcc tgggctgggt attgctggcg atgattcagc ttcctgcagt ctggctttcg
    22261 ctcggtattc tcggcaagaa gttcgcaatt cttgcgcgcc gctacaatgc ggtgacactg
    22321 aacgacatgc tgtttgcccg ctaccagagt cgtcttttgg tgtggctggc gagtttgagt
    22381 ttgctggtcg cgtttattgg cgcgatgacc gtgcagttta tcggcggcgc gcgcctgctg
    22441 gaaaccgcgg cgggtattcc ttatgaaacc gggctgctga ttttcggtat cagcattgcg
    22501 ttgtataccg cctttggtgg ctttcgcgcc agcgtgctga acgacactat gcaagggctg
    22561 gtgatgctga ttggcaccat tgtgctgctt attggcgtgg tacatgccgc aggtggctta
    22621 agcaacgcag tagagacgtt gcaaaccatc gatccgcaac tggttacgcc acaaggcgct
    22681 gacgatattc tgtcgcctgc ctttatgacg tcgttctggg tactggtgtg ctttggcgtg
    22741 attggcctgc cgcatactgc ggtgcgctgt atctcttata aagacagcaa agccgtacat
    22801 cgggggatca tcatcggtac gattgtggtc gcaatcctga tgttcggtat gcacctggcc
    22861 ggagcgttag gtcggacggt gatccccgat ctcaccgtac cggacctggt gatcccgacg
    22921 ttaatggtaa aagtgttgcc accgtttgct gccgggatct tcctggctgc acctatggct
    22981 gcgatcatgt cgacaattaa cgcccaactg ctgcaaagtt ccgctacgat cattaaagat
    23041 ctctatctga atatccgtcc ggatcaaatg caaaacgaga cgcgtctgaa gcggatgtcg
    23101 gcggtaatta cgttagttct cggtgcgttg ctgctgcttg ccgcctggaa gccgccagaa
    23161 atgatcatct ggctgaattt gctggccttc ggtgggctgg aagccgtttt cctgtggccg
    23221 ctggtactgg gtctttactg ggaacgcgcc aacgccaaag gcgcgctaag tgcgatgatt
    23281 gttggcggcg tactgtatgc cgtactcgcg acgctgaata ttcagtacct gggcttccac
    23341 cctatcgtgc cctcgttact actaagtttg ctggctttcc tggtcggaaa ccgtttcggt
    23401 acatccgtcc cgcaagctac cgttttgact actgataaat aaagagtttt gccatgcctt
    23461 ggatccaact gaaactgaac accaccggcg cgaacgcgga agatcttagc gatgcgctga
    23521 tggaagcggg tgccgtttct atcacttttc aggataccca cgatacgcct gtgtttgagc
    23581 cgctgccggg cgaaacgcgc ctgtggggcg acaccgatgt gattggtctg ttcgacgctg
    23641 aaaccgatat gaacgacgtg gtggcgattc tggaaaacca tccgctgctc ggcgcgggct
    23701 tcgcgcataa aatcgaacaa ctggaagata aagactggga gcgcgaatgg atggataatt
    23761 tccacccgat gcgctttggt gaacggctgt ggatctgccc aagctggcgt gatgtgccgg
    23821 acgaaaacgc cgtcaacgtg atgttagatc cagggctggc gtttggtacg ggtacccatc
    23881 caactacctc tctgtgcctg cagtggctcg acagcctcga tttaaccggt aaaacagtca
    23941 tcgactttgg ctgcggttcc ggcattctgg ctatcgcggc gctgaaactg ggtgcagcaa
    24001 aagccattgg tattgatatt gatccgcagg cgattcaggc cagccgcgac aacgcggaac
    24061 gtaatggcgt ctctgaccgt ctggaactct acttaccgaa agatcagcca gaagaaatga
    24121 aagccgacgt ggtggtcgct aacatccttg caggcccatt acgtgaactg gcaccgttaa
    24181 tcagcgtcct gccagtttca ggcggtttgc tgggcctttc cggtattctg gcaagccagg
    24241 cagagagcgt ttgcgaagct tatgccgaca gcttcgctct ggacccggtc gtggaaaaag
    24301 aagagtggtg ccgcattacc ggtcgtaaga attaaccttc gcatcgccgt agggtgacgc
    24361 agagcgaagt gcgagcgatg tcacaaaagg cacgttaatt tgccgattat ttacgcaaat
    24421 ttgcgtgcca aaattttcat tcataaagaa aaattgagaa cttactcaaa tttcttggag
    24481 tgtaaatttt agtcactatt ttctaatatg atgattttta tgagtaatta tcgcgccacg
    24541 ctcattttaa atgcaattct ttgatccatc tcagaggatt ggtcaaagtt tggcctttca
    24601 tctcgtgcaa aaaatgcgta atatacgccg ccttgcagtc acagtatggt catttcttaa
    24661 ctcatgcgca tcggacaata tcagctcaga aatcgcctga tcgcagcgcc catggctggc
    24721 attacagaca gaccttttcg gacgttgtgc tacgagatgg gagccggatt gacagtatcc
    24781 gagatgatgt cttctaaccc tcaggtttgg gaaagcgaca aatctcgttt acggatggtg
    24841 cacattgatg aacccggtat tcgcaccgtg caaattgctg gtagcgatcc gaaagaaatg
    24901 gcagatgcag cacgtattaa cgtggaaagc ggtgcccaga ttattgatat caatatgggt
    24961 tgcccggcta aaaaagtgaa tcgcaagctc gcaggttcag ccctcttgca gtacccggat
    25021 gtcgttaaat cgatccttac cgaggtcgtc aatgcagtgg acgttcctgt taccctgaaa
    25081 attcgcactg gctgggcacc ggaacaccgt aactgcgaag agattgccca actggctgaa
    25141 gactgtggca ttcaggctct gaccattcat ggccgtacac gcgcctgttt gttcaatgga
    25201 gaagctgagt acgacagtat tcgggcagtt aagcagaaag tttccattcc ggttatcgcg
    25261 aatggcgaca ttactgaccc gcttaaagcc agagctgtgc tcgactatac aggggcggat
    25321 gccctgatga taggccgcgc agctcaggga agaccctgga tctttcggga aatccagcat
    25381 tatctggaca ctggggagtt gctgcccccg ctgcctttgg cagaggttaa gcgcttgctt
    25441 tgcgcgcacg ttcgggaact gcatgacttt tatggtccgg caaaagggta ccgaattgca
    25501 cgtaaacacg tttcctggta tctccaggaa cacgctccaa atgaccagtt tcggcgcaca
    25561 ttcaacgcta ttgaggatgc cagcgaacag ctggaggcgt tggaggcata cttcgaaaat
    25621 tttgcgtaaa cagaaataaa gagctgacag aactatgttc gaacaacgcg taaattctga
    25681 cgtactgacc gtttctaccg ttaactctca ggatcaggta acccaaaaac ccctgcgtga
    25741 ctcggttaaa caggcactga agaactattt tgctcaactg aatggtcagg atgtgaatga
    25801 cctctatgag ctggtactgg ctgaagtaga acagcccctg ttggacatgg tgatgcaata
    25861 cacccgtggt aaccagaccc gtgctgcgct gatgatgggc atcaaccgtg gtacgctgcg
    25921 taaaaaattg aaaaaatacg gcatgaacta attcaggtta gctaaatgct tgattaaaaa
    25981 ggcgctactc ggcatgggga agcgcctttt ttataggtgt cacaaaggga gtgaccatga
    26041 gaacaggatg tgaaccgacc cgatttggta atgaagcaaa gaccatcatc cagggtgatg
    26101 cattaaccga acttaaaaag ctccccgccg aaagcgtcga tctgatcttt gccgacccac
    26161 cgtataacat cggtaaaaat tttgatggtc tgatcgaagc ctggaaagaa gatctgttta
    26221 tcgactggct gtttgaagtg attgcagagt gccaccgcgt tctgaaaaag cagggcagca
    26281 tgtacatcat gaacagcacg gaaaacatgc cctttatcga tctccagtgc cgcaagcttt
    26341 ttaccatcaa aagtcgcatc gtctggtcat atgacagttc aggagtgcag gcgaaaaaac
    26401 actacggctc catgtacgaa cccatcctga tgatggtgaa agacgcaaag aactacacat
    26461 tcaacggtga tgctgttctg gtagaagcca aaaccggatc gcagcgcgcg ttgatcgatt
    26521 atcgcaaaaa tcctccacag ccttacaatc atcaaaaagt accgggtaac gtctgggatt
    26581 ttcctcgcgt gcgttatttg atggatgaat atgaaaacca cccgacgcaa aaaccggaag
    26641 ccttactgaa acgcattatt ctcgcctctt ccaacccagg cgatatcgtt ctcgacccgt
    26701 ttgccggtag ctttactacc ggtgccgtag ccgtcgccaa cgggcgaaaa ttcattggta
    26761 ttgagatcaa cagcgagtac atcaaaatgg ggcttcgacg gctggatgtc gcgtcgcatt
    26821 actctgcgga agaactggcg aaagtgaaaa aaagaaagac cggcaacctg tcaaaacgaa
    26881 gccggttaag cgaagttgac cccgatctca ttgcaaagta aaggaagtgt aagcctggtt
    26941 ttcagattat tcatttcgtg tatatttcct gccagacttg gttaaacatg cacaggctca
    27001 ggtaatgatt cgcaagtatt ggtggctcgt cgttttcgct gtcgtcgttt tcgctgtctt
    27061 cgtttttctg tttgatactt tactgatgca gtggattgaa ctgctggcaa cagaaacaga
    27121 caaatgccgc aatatgaact cagttaatcc actaaaactg gttaactgtg acgaactgaa
    27181 ttttcaggac agaatgtgaa tttatatgac acttaattca ttcgttttat gaattaattt
    27241 cgttatgttt tcatctggca tgaacattct taatacgtta tcgaccagag cgggggcttg
    27301 tttataaaga tcataacccg ccatattcat taaccagttt tgaacaattc cgctgaaggc
    27361 accatcaata ataatcatca caacatctaa atcgagatta tttgctacac aaccttgttg
    27421 ctgacacgcc tgcaatactt cgcggagagt ctgcggatta aagcccatct tttcgcgtat
    27481 cactccctcg gccagcatct catcattaaa ttcacatttg tgatataaga ttttcagcaa
    27541 cgcctgctgg cggggaattt tggcaatata ttgcaaaccg acaatcaatt tttcacgcaa
    27601 ttgctgaaac gggtcatgct ctaacccagc ggtcaagtga tcctggatta actcccgcaa
    27661 tgaaggctgt tgcaaccaca tctcattaaa cagttgggtc ttgttctcga agtgccagta
    27721 gatagcgcca cgcgtaacgt tagcagcgtc ggcaatgtcg ttgagcgtcg tcttgcttac
    27781 gccatgttgc gcaaactggg cgatggcagt ttcaatcagt tcttgccggg tcttcagagc
    27841 ttcggctttg gttctttttg ccatgattaa ttattcagga aataaatata ttcgacacag
    27901 agtgagaaaa t
//
LOCUS       DAZKJR010000063        27535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKJR010000063 DAZKJR010000000
VERSION     DAZKJR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.063"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            142..384
                     /gene="yjbE"
                     /locus_tag="ACHOWS_002648"
     CDS             142..384
                     /gene="yjbE"
                     /locus_tag="ACHOWS_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFQ0048199.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAASGGGDGSNTGTTTTTTTSTQ"
     gene            571..1209
                     /gene="yjbF"
                     /locus_tag="ACHOWS_002649"
     CDS             571..1209
                     /gene="yjbF"
                     /locus_tag="ACHOWS_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFQ0048200.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWIEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1206..1943
                     /gene="yjbG"
                     /locus_tag="ACHOWS_002650"
     CDS             1206..1943
                     /gene="yjbG"
                     /locus_tag="ACHOWS_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFQ0048201.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLARLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1943..4039
                     /gene="yjbH"
                     /locus_tag="ACHOWS_002651"
     CDS             1943..4039
                     /gene="yjbH"
                     /locus_tag="ACHOWS_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFQ0048202.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4086..4364)
                     /locus_tag="ACHOWS_002652"
     CDS             complement(4086..4364)
                     /locus_tag="ACHOWS_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048203.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4578..4988
                     /gene="psiE"
                     /locus_tag="ACHOWS_002653"
     CDS             4578..4988
                     /gene="psiE"
                     /locus_tag="ACHOWS_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFQ0048204.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5082..5972)
                     /gene="malG"
                     /locus_tag="ACHOWS_002654"
     CDS             complement(5082..5972)
                     /gene="malG"
                     /locus_tag="ACHOWS_002654"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFQ0048205.1"
                     /translation="MAMVQPKSQKTRLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5987..7531)
                     /gene="malF"
                     /locus_tag="ACHOWS_002655"
     CDS             complement(5987..7531)
                     /gene="malF"
                     /locus_tag="ACHOWS_002655"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFQ0048206.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7685..8875)
                     /gene="malE"
                     /locus_tag="ACHOWS_002656"
     CDS             complement(7685..8875)
                     /gene="malE"
                     /locus_tag="ACHOWS_002656"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFQ0048207.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            9240..10355
                     /gene="malK"
                     /locus_tag="ACHOWS_002657"
     CDS             9240..10355
                     /gene="malK"
                     /locus_tag="ACHOWS_002657"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFQ0048208.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10427..11767
                     /gene="lamB"
                     /locus_tag="ACHOWS_002658"
     CDS             10427..11767
                     /gene="lamB"
                     /locus_tag="ACHOWS_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFQ0048209.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            12005..12925
                     /gene="malM"
                     /locus_tag="ACHOWS_002659"
     CDS             12005..12925
                     /gene="malM"
                     /locus_tag="ACHOWS_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFQ0048210.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGIRGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            13104..13601
                     /gene="ubiC"
                     /locus_tag="ACHOWS_002660"
     CDS             13104..13601
                     /gene="ubiC"
                     /locus_tag="ACHOWS_002660"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFQ0048211.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13614..14486
                     /gene="ubiA"
                     /locus_tag="ACHOWS_002661"
     CDS             13614..14486
                     /gene="ubiA"
                     /locus_tag="ACHOWS_002661"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFQ0048212.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14641..17064)
                     /gene="plsB"
                     /locus_tag="ACHOWS_002662"
     CDS             complement(14641..17064)
                     /gene="plsB"
                     /locus_tag="ACHOWS_002662"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFQ0048213.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPGVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17235..17603
                     /gene="dgkA"
                     /locus_tag="ACHOWS_002663"
     CDS             17235..17603
                     /gene="dgkA"
                     /locus_tag="ACHOWS_002663"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0048214.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17713..18321
                     /gene="lexA"
                     /locus_tag="ACHOWS_002664"
     CDS             17713..18321
                     /gene="lexA"
                     /locus_tag="ACHOWS_002664"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0048215.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18394..19719
                     /gene="dinF"
                     /locus_tag="ACHOWS_002665"
     CDS             18394..19719
                     /gene="dinF"
                     /locus_tag="ACHOWS_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFQ0048216.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            19835..20044
                     /gene="yjbJ"
                     /locus_tag="ACHOWS_002666"
     CDS             19835..20044
                     /gene="yjbJ"
                     /locus_tag="ACHOWS_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0048217.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(20086..20601)
                     /gene="zur"
                     /locus_tag="ACHOWS_002667"
     CDS             complement(20086..20601)
                     /gene="zur"
                     /locus_tag="ACHOWS_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFQ0048218.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20757..21752
                     /gene="dusA"
                     /locus_tag="ACHOWS_002668"
     CDS             20757..21752
                     /gene="dusA"
                     /locus_tag="ACHOWS_002668"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFQ0048219.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDVDPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21886..22128
                     /gene="pspG"
                     /locus_tag="ACHOWS_002669"
     CDS             21886..22128
                     /gene="pspG"
                     /locus_tag="ACHOWS_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFQ0048220.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(22294..23277)
                     /gene="qorA"
                     /locus_tag="ACHOWS_002670"
     CDS             complement(22294..23277)
                     /gene="qorA"
                     /locus_tag="ACHOWS_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFQ0048221.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            23360..24775
                     /gene="dnaB"
                     /locus_tag="ACHOWS_002671"
     CDS             23360..24775
                     /gene="dnaB"
                     /locus_tag="ACHOWS_002671"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0048222.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24828..25907
                     /gene="alr"
                     /locus_tag="ACHOWS_002672"
     CDS             24828..25907
                     /gene="alr"
                     /locus_tag="ACHOWS_002672"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0048223.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            26160..27353
                     /gene="tyrB"
                     /locus_tag="ACHOWS_002673"
     CDS             26160..27353
                     /gene="tyrB"
                     /locus_tag="ACHOWS_002673"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFQ0048224.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         6419 a         7004 c         7454 g         6658 t
ORIGIN      
        1 tatttgatat tcattccaat tatcctaaaa ccccatcact aattccccgc gccgtaattc
       61 gcatgcttta gttgtgtata ctcgatcccg cccgaaatgt ttttgggtaa atctccattc
      121 attcaatgaa gggaaattgt tatgaaaaaa gttctgtatg gcatttttgc catatctgcg
      181 cttgcggcga cttctgcgtg ggctgcacct gtacaggtgg gcgaagcggc agggtcggca
      241 gcaacgtcgg tttcggcggg gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg
      301 gtgggtgtcg cgctcgcggc aagcggtggc ggtgatggtt ctaataccgg gaccacaaca
      361 accacgacca ccagtaccca gtaataaagt atgtatcccc aaaataattc gagttgcagg
      421 aaggcgacaa gcgagtgagt ccccaggagc atagataact atgtgaccgg ggtgaacgag
      481 tgcggtcacc gcatctgcag cttgaagtat gaaggggatt aaccataacc acactccggt
      541 gtggttattc tgcccctctg gagaagagtc gtgaagcgac ctgcactcat tcttatctgc
      601 ctgctattac aggcctgttc agccacgact aaagagctgg gcaattcact gtgggacagt
      661 ctgttcggca cgccaggcgt acagctgacg gacgatgata ttcaaaatat gccctacgcc
      721 agccagtaca tgcagcttaa tggcgggccg cagttatttg tggtgctggc cttcgctgaa
      781 gacggacaac aaaaatgggt cactcaggat caggccactc tcgtcacaca acatggtcgt
      841 ctggtgaaga ctttgcttgg cggcgacaac ctgattgaag tgaataacct tgccgccgac
      901 ccgctgatta aacccgcaca aattgttgat ggtgcaacat ggacgcgcac gatgggctgg
      961 atcgagtacc agcaggtacg ctacgccacc gcacgctcag tcttcaaatg ggatggcacc
     1021 gataccgtca aggtcggcag cgatgaaacc ccggttcgcg tgctggacga agaagtctcc
     1081 accgaccagg cgcgctggca taaccgctac tggatcgaca gcgaagggca aattcgccag
     1141 tcggaacagt atctcggcgc ggattatttc ccggtgaaaa ccactctcat caaggcggca
     1201 aaacaatgat taaacaaact attgtcgcat tgattttgag tgtgggggcg tcatcggtct
     1261 tcgcggcagg aaccgtcaag gtgttcagca atggcagcag tgaggccaaa acgctaacgg
     1321 gcgcagagca tttaatcgat ctggtagggc aacctcgact ggcaaacagc tggtggcctg
     1381 gtgcagtgat cagcgaagag ctggcaacgg cggcagcatt gcggcagcag caggcgctac
     1441 tggcacggct ggcagaactg gccgcagatt ccagcgctga cgatgccgct gcgattaacg
     1501 ccttacgcca gcaaattcag gcgttgaaag tgacgggcag gcaaaaaatc aatcttgatc
     1561 ccgatatcgt gcgcgttgcc gaacgtggta acccaccgtt gcagggcaac tacacactgt
     1621 gggtcggacc accaccgtcc actgtcacgt tgttcgggct tatcagccgt cctggcaagc
     1681 agccattcac tccaggtcgc gacgtggcga gctatctctc cgggcagaac ctgcttagcg
     1741 gtgcggatcg cagctacgcg tgggtggttt acccggacgg acgcacgcaa aaagcgccgg
     1801 tggcttactg gaacaagcgt cacgtagagc cgatgcccgg cagcattatt tatgttggcc
     1861 tcgcggactc cgtctggagt gagacacctg atgcccttaa cgccgacatt cttcagactc
     1921 tgacgcagcg gataccccaa taatgaaaaa aagacatctg cttagcttac tggcgctggg
     1981 tattagcaca gcttgctacg gcgaaacata tcctgcgccc attggtccgt cgcaatcgga
     2041 tttcggtggc gtaggattat tacaaacgcc caccgcgcgc atggcgcggg aaggggagct
     2101 gagtctgaac tatcgcgata acgatcagta ccgttattac tcagcttcag tgcaactctt
     2161 cccgtggctg gaaacaacgc tgcgctacac cgacgtgcgc acccggcagt acagcagcgt
     2221 cgaagcgttc tctggcgatc aaacgtataa agataaagcc ttcgatctca aactgcgttt
     2281 gtgggaagag agttactggc tgccgcaagt ggcggttggt gcgcgggata tcggcggtac
     2341 ggggctgttc gatgcggaat atcttgtcgc cagcaaagcc tgggggccat tcgattttac
     2401 gctcggcctg ggctggggat atctgggcac cagcggtaat gtgaaaaatc cgctctgttc
     2461 ggccagtgac aaatattgct atcgcgataa cagctacaaa caagcgggat ctatcgacgg
     2521 cagccagatg ttccacggtc cggcctcact gtttggcggc gtggaatacc agacgccctg
     2581 gcaaccgctg cgcctgaaac tggagtatga aggcaataat tatcagcagg attttgccgg
     2641 taagctggag caaaaaagta agtttaacgt cggggcgatt tatcgcgtta ccgattgggc
     2701 cgacgttaac cttagctatg aacgtggcaa cacttttatg tttggcgtca cgctgcgcac
     2761 caactttaac gatctgcgcc cgtcctacaa cgataacgcc cgcccgcaat atcaaccgca
     2821 accacaggat gccattttgc agcattcggt ggtggcgaat cagttaacgc tgttgaaata
     2881 caacgccgga ctggccgatc cgcagatcca gacgaaaggc gatacgctgt atgtcaccgg
     2941 cgagcaggtg aaatatcgtg attcgcgtga agggatcatc cgtgctaatc ggatcgtgat
     3001 gaacgatctg ccggatggga tcaaaacgat ccgcattacg gaaaatcgcc ttaacatgcc
     3061 gcaggtgacg acggaaaccg atgtcgccag cctgaaaaat catctcgccg gagagccgtt
     3121 gggccacgaa acgaagctgg cgcaaaaacg cgtcgagcca gtggttccgc agtccaccga
     3181 gcagggctgg tatatcgaca aatcacgctt tgatttccat atcgatccgg tgttgaacca
     3241 gtcggtcggt ggcccggaaa acttttacat gtatcagctg ggcgtgatgg gaacggcgga
     3301 tttgtggctg acggaccatc tgctgaccac cggcagcctg tttgctaacc ttgccaacaa
     3361 ctacgacaag tttaactaca ccaatccgcc gcaggactcg cacttaccgc gcgttcgtac
     3421 ccatgtgcgc gagtatgtgc agaacgatgt ctatgtgaat aacctgcaag ccaactactt
     3481 ccagcatctg ggcaacggct tctacggtca ggtctacgga ggttatctcg aaaccatgtt
     3541 tggcggtgcg ggggcagaag tgttgtatcg cccgctggac agcaactggg cgtttggtct
     3601 ggatgccaac tacgttaaac agcgcgactg gcgtagtgca aaagatatga tgaaattcac
     3661 cgactacagc gtgaaaaccg gacatctgac cgcatactgg acgccatctt ttgctcagga
     3721 tgtgttagtg aaagcgagcg tcgggcagta tctggcaggg gataaaggcg gcacgctgga
     3781 gatcgccaaa cgctttgata gcggcgtggt ggtgggtggc tatgccacga tcaccaacgt
     3841 ttcgaaagag gagtacggcg aaggggactt taccaaaggc gtgtatgtct ctgtaccgct
     3901 ggatctcttc tcgtctggcc cgacacgtag ccgtgcggca attggctgga cgccgctgac
     3961 gcgtgacggt ggtcagcaac ttgggcgtaa gttccagttg tatgacatga ccagcgaccg
     4021 tagcgtcaat ttccgctaag tcatggaaaa ggtgccagtt ttcgcactca ctggtaccct
     4081 cattcttaat ggcctgtcga gccatcagga acgcagttca cccccggttt gcgatctggt
     4141 gtgtaaaggc gaaaacattc tgtcttcctg tccgtcgata tcggttgcag gtctggttgc
     4201 ctgagcgact gggccatcat tggactggag acattgcttt gcggtaggtc ggctttatca
     4261 gcagtaccga gcggaccagc ataagcagga agaacagaga ctgataacat caaagcagca
     4321 aaataaggct tcatttttac cacctttatc aggttacgtt tcatttgttc cagaggaaca
     4381 ttgtcgattt ttcgcgcatt gctggtggct gggaatcacc tgaatgggtg atttttttgg
     4441 ctgtgtgttt tgccagatga gacattgatg ggagatgccc acttaattga gctgaacaaa
     4501 acatacacaa aaaatataga tctccgtcac atttttgcgt tatacaggaa gctcgccact
     4561 gtgaaggagg tactgctatg acgtcactct ctcgtccgcg cgtggagttt atatccacca
     4621 ttttgcagac cgtactcaat cttggcctgt tgtgcctcgg cctgattttg gttgtcttcc
     4681 tcggcaaaga aacggtgcat ctggctgatg tgctgttcgc gccagaacaa accagtaaat
     4741 atgagctggt agaagggctg gtggtttact ttctctattt cgaatttatc gcgctgattg
     4801 tgaagtactt tcagtccggt tttcacttcc cgctacgtta ctttgtctat attgggatca
     4861 ccgccattgt gcgcttgatc atcgtcgatc ataaatcgcc actggatgtg ttgatctact
     4921 cggcagcgat cctgttactg gtgatcaccc tgtggttgtg taattcgaaa cggctgaagc
     4981 gggagtaaaa aaaggcgctc cgtggagcgc cgaataacag tcacaagttg ggataacgta
     5041 agttgagggt gcagcggcat aacattggca gaacaacatc tttaaccttt cacaccacct
     5101 gccgtcaggc cgttgaccag ccagcgttga gccagcaaga agacgatggt gatcggtaat
     5161 gcagacatca cggcagcggc ggcaaagtca ccccacaggt agttttgcgg gttgaggtat
     5221 tgctgcatcc ccacagccag ggtgtagctg tttacgtcac gcagtaatag cgacgcgacc
     5281 ggaacttcag taatggcagc gataaacgac aggatgaaca caaccgccag aatcggtact
     5341 gacagcggca acaggacaag gcggaaggcc tgccacggtg tcgcaccatc cagcgccgcc
     5401 gcttcttcca gcgaactgtc gatggtttcg aaataacctt tgatggtcca gacatgcagc
     5461 gcaatcccgc ccagatacgc gaaaattacg ccgccgtgag tattcaggcc aatgaatgga
     5521 atgtactcac ccagacgatc aaacaacgca tacaacgcga ccagtgaaag tactgccggg
     5581 aacatctgga aaatcagcat tcctttcagc agcgtcgctt tgcctggaaa gcgcatacgg
     5641 gcgaaagcgt aggcgcaggt ggtggagagc gccacaatgc caatcgcgga aatcccggcg
     5701 acctttaccg agttccacag ccatagcagt accggaaacg gcggcggtgt aacgcgacca
     5761 tcagcctgtt ccacgctaaa acctaatgcc agtttccagt gatcccagga gatttgctcc
     5821 gggatcaggc tgccggtcgc aaagttcccc tggcgcagtg agatagcgac gaccatcagt
     5881 agcgggaaca taatcgccgc gataaaaagt agcagtagca ggtgagtaat aaacaaacgt
     5941 gttttttgcg atttcggttg gaccattgcc atttttgtta tctcccttaa tcaaacttca
     6001 ttcgcgtggc tttcaggttc actatcgcca gcgcacccac cagcaggaag atcagcgtgg
     6061 caattgctgc cgccagaccg aagtcctgac ccccgccgcc ttcaaaagcg atgcggtagg
     6121 tgtagttaac aagcaggtcg gtataaccgg ctggcgtggt cgtgccaaga cgatccgggc
     6181 cgccgttggt taacagttga atcagcacga agttgttaaa gttaaaggcg aagctggcga
     6241 tcatcagcgg cgtcagcggt ttaatcagca gcggcagcgt aatcttaaag aagttctgga
     6301 acggacctgc gccatccatt gctgaggctt catacaaatc gtccggaatc gctttcagca
     6361 agcccatgca gaggatcatc atgtacggat aacccagcca ggtattgacg ataatcagca
     6421 tcgttcgggc ggtggtcgga tcgctgaacc aggcgggctt cacgccaaac agcgcgctca
     6481 acatcatgtt gatctcaccg aagctctggt taaacaaccc tttgaaaatc aagattgaaa
     6541 tgaacgatgg caccgcgtag ggcagaatca gcaggacgcg atagaccgct ttgccgcgca
     6601 acgcttccca ctgcaccaga cacgccagaa ccatgccgac cgccaccgtt aaaaagacag
     6661 tgatcagcga gaacaccacg gtccagacga aaatggcgag gaacggtttc tgaatgcctt
     6721 cgtcggtgaa gacgcgggta aagtttttcc agccagtggt cacggtgtaa ccggggctta
     6781 gcttttcatc gccccagttg ccgtcggcgg taatggactg gtaaaagcca atttggttat
     6841 tcggacgata tttcacgccg ctctgattat tcgtcaacgt gccgtcaccg tcgagtgtgt
     6901 agagcggctg cgtgccagaa aactggcgca gggagctcat catcactttg ttgccatccg
     6961 gcagaatggc ggtaatgtca ctcagcgcct gacgattctg ggtaatcacg cgtagattcg
     7021 cgcgttcgcc ttcgggctgg gcggtcgttt ctttcagttg cagtttttgc tcgccgccaa
     7081 atttaaaagc gtcggaaagg taatttttgc cggtttcgcc gtcgctgagc gccagttgcc
     7141 actcatcgcc cgccgggtaa agaccaaagt tataggtttt gcctgcttgc caggagcgat
     7201 ctaacaacac ttcctgtgca cgttcaaaag tcagctggtt agtgctgctg tagttggtga
     7261 aggcaatggc gatggtgcag accagaggga agaggacgaa taatcccatt ccagccattc
     7321 ccgggtaaac atagcgccag gcgtaggctt tacgattggc gaaaatatac agccccgctg
     7381 aactcaatat cagcgtggta atggcgaaca ggtattcccc ttgtgcgtac attaaaacaa
     7441 caaggtaacc caccagcagg ccgagcagac ctaacactga ccatttcagc gcgtcgcttt
     7501 gccaccaatg tttcttttta atgacatcca tggggttctt cctcattcca ggacggataa
     7561 ggctttcacg ccttatccga caacaactgc ctgatgcgac gctgacgcgt cttatcaggc
     7621 ctacatacgt ttcaatttcg taggccggat aaggcgttta cgccgcatcc ggcatttcac
     7681 agcattactt ggtgatacga gtctgcgcgt ctttcagggc ttcatcgaca gtctgacgac
     7741 cgctggcggc gttgatcacc gcagtacgca cggcatacca gaaagcggac atctgcggga
     7801 tgttcggcat aatttcacct ttctgagcgt tatccatggt ggcggcaata cgtggatctt
     7861 tcgcaagttc atcctgatag gacttcagcg ctactgcacc cagcggtttg tctttattga
     7921 ccgcttccag accttcgtca gtcagcagat agttttcgag gaactctttc gccagctctt
     7981 tgttcggact ggcggcgtta atacctgcgc tcagcacgcc aacgaacggt ttagatggtt
     8041 gacctttgaa ggtcggcagt accgttacac cataattcac tttactggtg tcgatgttgg
     8101 accatgccca cgggccgttg atagtcatcg ctgtttcgcc tttattaaag gcagcttctg
     8161 cgatggagta atcggtgtct gcattcatgt gtttgttttt aatcagatca accaggaagg
     8221 ttagacccgc tttcgcgcca gcgttatcca cgcctacgtc tttaatgtcg tacttgccgt
     8281 tttcatactt gaacgcataa cccccgtcag ccgcaattag cggccaggtg aagtatggtt
     8341 cttgcaggtt gaacatcagc gcgctcttac ctttcgcttt cagttcttta tccagcgccg
     8401 ggatctcttc ccaggttttt ggcgggttcg gtagcagatc tttgttataa atcagtgata
     8461 acgcttcgac agcgatcggg taagcaatca gcttgccgtt gtaacgtacg gcatcccagg
     8521 taaacggata cagcttgtcc tggaacgctt tgtccggggt gatttcagcc aacaggccag
     8581 attgagcgta gccgccaaag cggtcgtgcg cccagaagat aatgtcaggg ccatcgccag
     8641 ttgccgcaac ctgagggaat ttctcttcca gtttatccgg atgctcaacg gtgactttaa
     8701 ttccggtatc tttctcgaat ttcttaccga cttcagcgag accgttatag cctttatcgc
     8761 cgttaatcca gattaccagt ttaccttctt cgattttggc gagagccgag gcggaaaaca
     8821 tcatcgtcgt taatgcggat aatgcgagga tgcgtgcacc tgtttttatt ttcataatct
     8881 atggtccttg ttggtgaagt gctcgtgaaa acacctaaac ggactctagt ttctttatac
     8941 ggcaacctct ttccatcctc cttgccccta cgccccaccg ttgctttgtg tgatctctgt
     9001 tacagaattg gcggtaatgt ggagatgcgc acataaaatc gccatgattt ttgcaagcaa
     9061 catcacgaaa ttccttacat gacctcggtt tagttcacag aagctgcgtt ctcatcctcc
     9121 cgcctcctcc cccataaaaa agccaggggg tggaggattt aagccatctc ctgatgacgc
     9181 atagtcagcc catcatgaat gttgctgtcg atgacaggtt gttacaaagg gagaagggca
     9241 tggcgagcgt acagctgcaa aatgtaacga aagcctgggg cgaggtcgtg gtatcgaaag
     9301 atatcaatct cgatatccat gaaggtgaat tcgtggtgtt tgtcggaccg tctggctgcg
     9361 gtaaatcgac tttactgcgc atgattgccg ggcttgagac gatcaccagc ggcgacctgt
     9421 tcatcggtga gaaacggatg aatgacactc cgcctgcaga acgcggcgtt ggtatggtgt
     9481 ttcagtctta cgcgctctat ccccacctgt cagtagcaga aaacatgtca tttggcctga
     9541 aactggctgg cgcgaaaaaa gaagtgatta accagcgagt taaccaggtt gcggaagtgc
     9601 tgcaactggc gcatttgctg gatcgcaaac cgaaagcgct ctccggtggt cagcgtcagc
     9661 gtgtggcgat tggccgtacg ctggtagccg agccaagcgt atttttgctc gatgaaccgc
     9721 tctccaacct cgatgctgca ctgcgtgtgc aaatgcgtat cgaaatctcc cgtctgcata
     9781 aacgcctggg ccgcacaatg atttacgtca cccacgatca ggtcgaagcg atgacactag
     9841 ccgacaaaat cgtggtgctg gacgccggtc gcgtggcgca ggttgggaaa ccgctggagc
     9901 tgtaccacta tccggcagac cgttttgtcg ccggatttat cggttcgcca aagatgaact
     9961 tcctgccagt aaaagtgacc gccactgcaa tcgatcaagt gcaggtggag ctgccgatgc
    10021 caaatcgtca gcaagtctgg ctgccggttg aaagccgtga tgtccaggtt ggagccaata
    10081 tgtcgctggg tattcgcccg gaacatctac tgccgagtga tatcgctgac gtcatccttg
    10141 agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac ccaaatccat atccagatcc
    10201 cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt ggtgttggta gaagaaggtg
    10261 ccacattcgc tatcggcctg ccgccagagc gttgccatct gttccgtgag gatggcactg
    10321 catgtcgtcg actgcataag gagccgggcg tttaagcacc ccacaaaaca cacaaagcct
    10381 gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga gatagaatga tgattactct
    10441 gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta atgtctgctc aggcaatggc
    10501 tgttgatttc cacggctatg cacgttccgg tattggctgg acaggtagcg gcggtgaaca
    10561 acagtgtttc cagactaccg gtgctcaaag taaataccgt cttggcaacg aatgtgaaac
    10621 ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagag ggcgataaga gcttctattt
    10681 cgacactaac gtggcctatt ccgtcgcaca acagaatgac tgggaagcta ccgatccggc
    10741 cttccgtgaa gcaaacgtgc agggtaaaaa cctgatcgaa tggctgccag gttccaccat
    10801 ctgggcaggt aagcgcttct accaacgtca tgacgttcat atgatcgact tctactactg
    10861 ggatatttct ggtcctggtg ccggtctgga aaacatcgat gttggcttcg gtaaactctc
    10921 tctggcagca acccgttcct ctgaagctgg tggttcttcc tctttcgcca gcaacaatat
    10981 ttatgactat accaacgaaa ccgcgaacga cgttttcgac gtgcgtttag cgcagatgga
    11041 aatcaacccg ggcggcacat tagaactggg tgtcgactac ggtcgtgcca acctgcgtga
    11101 taactatcgt ctggttgatg gcgcatcgaa agacggttgg ttattcactg ctgaacatac
    11161 tcagagtgtc ctgaagggct ttaacaagtt tgttgttcag tacgctactg actcgatgac
    11221 ctcgcagggt aaaggtctgt cgcagggttc tggcgtcgcg tttgataacg aaaaatttgc
    11281 ctacaatatc aacaacaacg gtcacatgct gcgtatcctc gaccacggtg cgatctccat
    11341 gggcgacaac tgggacatga tgtacgtggg tatgtaccag gatatcaact gggataacga
    11401 caacggcacc aagtggtgga ccgtcggtat tcgcccgatg tacaagtgga cgccaatcat
    11461 gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc cagcgcaccg gcgacaagaa
    11521 caatcagtac aaaattaccc ttgcacaaca atggcaggct ggcgacagca tctggtcacg
    11581 cccggctatt cgtgtcttcg caacctacgc caagtgggat gagaaatggg gttatgacta
    11641 caccggtaac gccaatacca acactaactt cggcaaagcc gttcctgctg atttcaacgg
    11701 cggcagcttc ggccgtggtg acagcgacga gtggaccttc ggtgcccaga tggaaatctg
    11761 gtggtaatag caaaacctgg gccggataag gtgcttgcgc cgcatccggc aaccaacgcc
    11821 tgatgcgacg cttgcgcgtc ttatcaggcc tacaactaca accgctgccg aaagtaggcc
    11881 ggataaggcg tttacgccgc atccggcata aaaacagctt taaggggcga aagcccctct
    11941 gattatcggg tttagcgcgc tattgcctgg ctaccgctga gctccagatt ttgaggtgaa
    12001 aacaatgaaa atgaataaaa gtctcatcgc tctctgttta tcagcagggt tgctggcaag
    12061 cgcacctgga atcagccttg ccgatgttaa ctacgtaccg caaaacacca gcgacgcgcc
    12121 agccattcca tctgccgcgc tgcaacaact cacctggaca ccggtcgatc aatctaaaac
    12181 ccagaccacc caactggcga ctggcggcca acaactgaac gttcccggca tcagaggtcc
    12241 ggttgctgcg tacagcgtcc cggcaaacat tggcgaactg accctaacgc tgaccagcga
    12301 agtgaacaaa caaactagcg tatttgcgcc gaacgtgctg attcttgatc agaacatgac
    12361 cccatcagcc ttcttcccca gcagttattt cacctaccag gaaccaggcg tgatgagtgc
    12421 agatcgtctg gaaggcgtta tgcgcctgac accggcgttg gggcagcaaa aactttatgt
    12481 tctggtcttt accacggaaa aagacctcca gcagacgacc caactgctcg acccggctaa
    12541 agcctatgcc aaaggcgtcg gtaactcgat cccggatatt cctgacccgg ttgctcgtca
    12601 taccaccgat ggcttactga aactgaaagt caaaacgaac tccagttcta gcgtgttggt
    12661 cgggcctcta tttggttctt ccgctccagc tccggttacg gtaggcaaca cggcggcacc
    12721 agctgtggct gcacccgctc cggcaccggt gaagaaaagc gagccgatgc tcaacgacac
    12781 ggaaagttat tttaataccg cgatcaaaaa cgctgtcgcg aaaggtgatg ttgataaggc
    12841 gttaaaactg cttgatgaag ctgaacgcct ggggtcgaca tctgcccgtt ccacctttat
    12901 cagcagtgta aaaggcaagg ggtaattacg ccccacagtg ctgattttgc aacaactggt
    12961 gcgtctcctg gcgcaccttt ttttctcctc ctgtactatt gttgcgtttt tgttgcgcat
    13021 tagttcactt aatttgcttt acatctcccg taaatatttt tctgcgatac aatgccttta
    13081 cgttatgtaa cggagagttc ggcatgtcac accccgcgtt aacgcaactg cgtgcgctgc
    13141 gctattttac agagatccct gccctggagc cgcaactgct cgactggctg ttgctggaag
    13201 attccatgac aaaacgtttt gaacagcagg gaaaaacggt gagcgtgacg atgatccgcg
    13261 aagggtttgt cgagcagaat gaaatccccg aagaactgcc actgctgccg aaagagtctc
    13321 gttactggtt acgtgaaatt ttgttatgtg ccgatggtga accgtggctt gccggtcgta
    13381 ccgtcgttcc tgtgtcaacg ttaagcgggc cggagctggc gttacaaaaa ttaggtaaaa
    13441 cgccgttagg acgctatctg ttcacatcat cgacattaac ccgggacttt attgagatag
    13501 gccgtgatgc cggactgtgg gggcgacgtt cccgcctgcg attaagcggt aaaccgctgt
    13561 tgctaacaga actgttttta ccggcgtcac cgttgtacta agaggaaaaa aatatggagt
    13621 ggagtctgac gcagaataag ctgctggcgt ttcatcgctt aatgcgtacg gataagccaa
    13681 ttggcgcgtt actgctgctc tggccaacat tatgggcgtt gtgggtggcg acaccgggcg
    13741 ttccccagct ctggatcctg gcggtgtttg tcgcgggcgt ctggctgatg cgcgctgctg
    13801 gttgcgtggt gaatgattat gctgaccgca agtttgatgg tcatgttaag cgcacggcga
    13861 accgaccact ccccagcggc gcggtaacgg agaaagaggc gcgcgcgctg tttgtagtgc
    13921 tggtactgat ttcattttta ctggtgctga cgctgaatac gatgactatt ctgttgtcga
    13981 ttgccgcgct ggcgctggcg tgggtatacc cgtttatgaa gcggtatacc catctaccgc
    14041 aagtggtgct gggcgcggcg tttggctggt cgattccaat ggcttttgct gctgtgagtg
    14101 agtcggtgcc attgagttgc tggttaatgt tcctcgccaa tattctctgg gcggtggcat
    14161 acgacacgca gtatgcgatg gttgaccgtg atgatgatgt gaagattggc attaaatcca
    14221 ccgcaatcct gttcggccaa tacgataaat tgattattgg tattttgcag attggcgtac
    14281 tggcgctgat ggcgattatc ggtgagttaa atggcttagg ctggggatat tactggtcaa
    14341 ttctggtggc tggcgcgctg tttgtttatc aacaaaaact gattgccaac cgcgagcgtg
    14401 aagcctgctt taaagcattt atgaataata actatgttgg tctggtactg tttttagggc
    14461 tggcaatgag ttactggcat ttctgatgat gtaaaaaagc cggatgatca tccggcttcc
    14521 ttctgggttg cctgatgcgc tgcgcttctc aggcctacac aacatatcgc aatttattga
    14581 atttgcagat catggtaggc cgaataaggc gtttgcgccg catccggcaa ttctccctga
    14641 ttacccttcg ccctgcgtcg cactctcaat cgtcaaacgc acgtctgatg taatcaactc
    14701 cgccagcaac tgatacacct tcatcgtttc tgccggttcg gcgtcgccgc tatcgctgat
    14761 atacccttca tcacgcagtg tcagcaccag agaactgaac accgccttgt cgaagaactc
    14821 cggcgcgttg atgccgtgca gcacggagag acgttgcgcg acggtgcggc tctctttctc
    14881 cagcgtaccg cggttgatcg acgggttggc gctcaacagc cagaaggtga tggcataacg
    14941 ttgcagcgtt tcgcgcgcgc ctgcggccag cagctgtagc gtgcgagaat gcgccgggtt
    15001 gatatgcaac tcatcatctt gcagggtaat cagcccctga cgttgcatct catttgccag
    15061 tgcatcaata acgcccggca actcgtcgcg atcccagcgc aggaacagct ccgctttcag
    15121 cattgggtaa agcacattga cgtgctccat caatacgtcg cgggagatgt ggcgatgctg
    15181 ggtgacaatt gccgccatca gcgaaggcag caccaacata tgcgcaatgt tgttgcgata
    15241 gtaggtcatc agcaccgctt gctcgcgcgg cagaatgatg atgtcgccga tcgtgtcttt
    15301 ctcgacttca aacttgttca tttgcagcgc gtgatcgata agctcgctgg cgctggctga
    15361 aggaacggta gagtccgtgg agtaaggcac gttgcgcatc agatccaggt agcagttgag
    15421 ttgctcggtt aactgctcgc gggtgagtga gcgctgacgt gacgccagaa gcgcagtaca
    15481 acacaggttc atggcgtttg ccgcgcctgc gttgttaatg cgtaccatca gatcggcagc
    15541 aatattattg accgtcggcg ttaaccatgc cggacgcacc gcttcgatgg gatcgataga
    15601 ttcacgccag tcaggtacat gctggttaag gtaggtcatc aacggcattg gttcaccgaa
    15661 gttgacgtaa ccctgaccga gattacgcag cttgcttaaa ccgcgcagca tctgcggcag
    15721 gctctctttc tctttcgtcg caccgcgcag ttctttggcg taagtaccca cttccatgac
    15781 gtgctcataa ccgatataga tcggaatcag cgtaatcgga cgcgtgccgc cacgcagcat
    15841 cgcctgaatg gtcatcgaca gcgtaccggt tttcggatcc agcaaacgcc ctgtacggga
    15901 acgaccgcct tccacgaaat actcgacgga ataaccacgg ctgaacagtt cgccgagata
    15961 ctcacggaaa acggtggaat agagcttatt gcctttaaac gtacggcgaa taaagaacgc
    16021 ccccagacgg cggaaaatcg gcccggcagg ccagaaattc aggttgatcc cggcggcgat
    16081 atgcggcggc accagcccct ggtgatacag cacgtaagaa agcagcaggt agtccatgtg
    16141 gctgcggtgg caaggcacat acaccagctc atgaccgtcg tgggccagct gacgaacgcg
    16201 ctcggcgtta tggacgttga tgccctggta aaggcggttc caggtgaagc ccagaatacg
    16261 gtcagtcagg cggatcatct cgtaagagaa attcgccgca atctcttcca tcagcgcaat
    16321 cgcgttctgc tgcgcttttt catgggagat ttttttgctg cgcgcttcat cttctaccgc
    16381 tttggcaatg gcgcgggagg cgagcagctt attaaacaga tcctgacgag caggcagacg
    16441 tgggcctacg gcagccagac gttgacgggc aaagtgcata cgcgccacgc gcgccagttt
    16501 ctgcgcgata gttttatccg tgccgtgttc atccgccata cggcgcagcg aaactgacgg
    16561 cgagaaacgc acaaaactgt cgcgaccgag ccacagtaca gcgaaaaatt tctgtacgcc
    16621 gttaagcata cgcagcggcg ggttcacttc gcctttttca cgccccggcg cgcgaccaaa
    16681 catcaccgat actggcacca tctgcacatc cagatttggg ttgctacggt gcaaatcgag
    16741 atagtcgtgg aacagcttaa tagactcttc tttcggcgtg taataggtga acacacgcgg
    16801 cccgccgtga atgaacacat agcgcggcag tagcgtgccg tcgatttcca gcggctctaa
    16861 cgggtcaggc aggtcatgtg ccagacactg ggcgcgcaac gtcagcaagt cagctttcga
    16921 gttgtacggt aaaacgtaca taattggacg agaggtatcc agccccagtt ccggggcagg
    16981 atctgccgga atagacttgc tttttaccag gatgcttaat ggtaaattca gtaatttgta
    17041 gtaaattcgt ggccagccgg acataaacga tgtaaagcct ctggttaata atgcaaatgc
    17101 gcggcaagga tagcagaaag tcatgggaaa ttctgtggta tccgctcatg tttcgcgcgg
    17161 cgttacgcaa acccgaatca tcggatttaa cggtacactg atattgacgc tcataatgta
    17221 aaaaggttct ttcaatggcc aataatacca ctggattcac ccgaattatc aaagctgctg
    17281 gctattcctg gaaaggttta cgcgctgcat ggatcaacga agcggcattc cgtcaggaag
    17341 gcgtagcggt attgttggcg gtggtcatcg cctgctggct ggatgtggac gcaattaccc
    17401 gcgtgctgct tatcagctcc gtgatgctgg tgatgattgt ggaaatcctc aatagcgcca
    17461 tcgaagcagt ggttgaccga attggctctg aataccatga gctttccgga cgcgctaaag
    17521 atatgggatc cgccgcggtt ctgattgcga ttatcgtcgc cgtgattacc tggtgcatcc
    17581 tgttatggtc gcattttgga taacccttcc agatttcgat aaatctctgg tttattgtgc
    17641 agtttatggt tccaaaatcg ccttttgctg tatatactca cagcataact gtatatacac
    17701 ccagggggcg gaatgaaagc gttaacggcc aggcaacaag aggtgtttga tctcatccgt
    17761 gatcacatca gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca gcgtttgggg
    17821 ttccgttccc caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa aggcgttatt
    17881 gaaattgttt ccggcgcatc acgcgggatt cgtctgttgc aggaagagga agaagggttg
    17941 ccgctggtag gtcgtgtggc tgccggtgaa ccgcttctgg cgcaacagca tattgaaggt
    18001 cattatcagg tcgatccttc cttattcaag ccgaatgctg atttcctgct gcgcgtcagc
    18061 gggatgtcga tgaaagatat cggcattatg gatggcgact tgctggcagt gcataaaact
    18121 caggatgtac gtaacggtca ggtcgttgtc gcacgtattg atgacgaggt taccgttaag
    18181 cgcctgaaaa aacagggcaa taaagtcgaa ctgttgccag aaaatagcga gtttaaacca
    18241 attgtcgttg accttcgtca gcagagcttc accattgaag ggctggcggt tggagttatt
    18301 cgcaacggcg actggctgta acatatctct gagaccgcga tgccgcctgg cgtcgcggtt
    18361 tgtttttcat ctctcttcat caggcttgtc tgcatggcat tcctcacttc atctgataaa
    18421 gcactctggc atctcgcctt acccatgatt ttctccaata tcaccgttcc gttgctggga
    18481 ctggtcgata cggcggtaat tggtcatctt gatagtccgg tttatttggg cggcgtggcg
    18541 gttggtgcaa cggcgaccag ctttctcttt atgctgttgc tgtttttacg catgagcacc
    18601 accgggctga ctgcgcaggc ttatggtgcc aaaaatcctc aggcattagc ccgtgcgctg
    18661 gtgcaaccgt tgctgttggc gttgggggct ggggcgttaa ttgcgctgct gcgtacgccg
    18721 attatcgatc tggcgctgca tattgttggc ggcagcgaag cggtgctaga acaggcgcga
    18781 cgctttcttg aaatccgctg gttaagtgca ccggcgtcgc tggcgaatct ggtattactt
    18841 ggttggttac tcggtgtgca atatgcccgt gcgccagtaa ttttgttagt ggtcggcaat
    18901 atcctcaaca ttgtgctgga tgtctggctg gtgatggggc tgcatatgaa cgtgcagggc
    18961 gcggcgctgg cgacggttat tgcggaatat gcaacattgc tgattggtct gctaatggtg
    19021 cgtaaaatcc tcaaactacg cggaatctcc ggcgaaatgc tgaaaactgc ctggcgagga
    19081 aacttccgtc acttgctggc gcttaaccgc gatatcatgc tgcgctcgct gttgttgcaa
    19141 ctctgtttcg gcgcgatcac cgtacttggc gcgcgactgg ggagtgacat tatcgctgtt
    19201 aacgcggttc tgatgacgtt actcaccttt accgcctatg cgctggatgg ttttgcctac
    19261 gcggttgaag cgcattccgg tcaggcgtac ggtgcgcgcg atggtagcca gttactggat
    19321 gtctggcggg cagcgtgccg ccagtcgggg atcgtagcgt tactgttttc ggtggtttat
    19381 ttgctggcag gggaacacat cattgcgttg ctgacgtcgt taacccagat tcagcagctg
    19441 gctgaccgct atcttatctg gcaggtgatt ttgccgttgg tcggcgtctg gtgttatctg
    19501 ctcgacggca tgtttatagg tgcaacgcgc gccgccgaaa tgcgtaacag tatggcggtg
    19561 gccgccgcag gttttgcgct gacgctcctt acgctgccgt ggctggggaa tcatggtttg
    19621 tggctggcat taaccgtctt tctggctttg cgcgggcttt ctctggcggc tatctggcgg
    19681 cgtcactggc gcaacggtac ctggtttgcc gcaacgtgac ggttaaaaat tctgaataaa
    19741 taatcctaag ccaaattgct gactacactt aatctcacgt tcagaagaaa agtgaacgta
    19801 ctctcattca caacctaacg atgaggtctt gattatgaat aaagatgaag ccggcggtaa
    19861 ctggaaacag tttaaaggta aagtgaaaga gcaatggggc aaactgaccg atgatgatat
    19921 gacgatcatt gaaggtaaac gtgatcaact ggtcggtaaa atccaggaac gttatggtta
    19981 tcagaaagat caggcagaaa aagaggttga tagttgggaa aaacgccatg attaccgctg
    20041 gtaattaatc cctcctgccc gacatgtaca aggatgtaca cccttttagc gaggtttctt
    20101 tttcacctga atagagtggt catgatggca ctgttcggga tgacgacacg cttctacttc
    20161 tacacatgct gaacataatc catgtgcttc aataacatta tgccgcaggg caaaacccat
    20221 ttttgccgcc agcgtatgca tgatgtcttc cacgccttca gcacattctt ctttcaccgc
    20281 gccgcagcga tcgcaaataa acatggctga agtatgggtg ggctgatcga acagatgaca
    20341 gagcacataa ctgttggtgg attccacctt atgcacaaaa ccttgttcaa gcagaaactc
    20401 cagcgcgcga taaaccgttg gcggcttggc ttgcggttca gcttcgcgca gcaaatcaag
    20461 cagatcataa gcgctgatag cgccatcctg cagactcatc aggcgcaata cttccaggcg
    20521 ctgtggggtc aggcgcacat tacgctgtgc gcagattttt tcagcctgcg ctaataactc
    20581 ctgcgttgtg gtcttttcca ttgggcacct cgacagtggg gttaagaatg tccttacttt
    20641 accatagttc tcgcttaaac gccgagaaca gggtgtcatg gctgtcacat tgcctgatcg
    20701 catattcgtt atgctatagt agcgccccca atttaccaga tgcttaaaaa gtcgccatgc
    20761 cagcagaatc acacactgtt tatcccgctt accgtttctc gattgccccg atgcttgact
    20821 ggacggacag acattgccgc tatttcttgc gtctgctttc ccgcaatacg ttgctgtata
    20881 ccgaaatggt gactacaggg gcgattattc acggtaaagg tgattacctg gcgtacagtg
    20941 aagaagaaca tccggtagcg ttgcaactcg ggggtagcga tccggcggcg ctggcacagt
    21001 gtgcgaagct ggcagaagcg cgcggatatg atgagatcaa cctgaatgtc ggctgcccgt
    21061 ctgaccgggt gcagaacggc atgtttggtg cgtgtctgat gggtaatgcg cagctggttg
    21121 ccgactgcgt gaaagcgatg cgcgatgtgg tgtcgattcc ggtgacggtg aaaacgcgta
    21181 ttggcatcga tgatcaggac agctatgaat ttctctgcga tttcatcaac accgtttccg
    21241 gcaaaggtga gtgtgagatg ttcatcatcc acgcacgtaa agcctggctt tcggggttaa
    21301 gcccgaaaga aaaccgtgaa atcccgccgc tcgattatcc gcgtgtgtat caactgaagc
    21361 gtgactttcc gcatctgacg atgtcgatta acggtggcat caagtcgctg gaagaggcta
    21421 aagcgcattt gcaacatatg gatggcgtga tggtcgggcg cgaagcgtat cagaatccag
    21481 gcattctggc ggcggtagac cgagagatct ttggttcttc ggataccgat gtcgatccgg
    21541 tggcggtggt gcgtgccatg tatccgtaca ttgagcgtga actcagccag gggacgtatc
    21601 tcggccatat tacccggcat atgctgggct tgttccaggg tattcctggc gcgcggcagt
    21661 ggcggcgtta tttaagtgaa aatgcccata aagcgggtgc tgacattaac gtgctggaac
    21721 acgcgctcaa actggtggcg gataagcgtt aacttttcac caaaaagtag tcaaattcac
    21781 cacgccctgc gcaccgtcgc ggggcgtttt gctgttaaat caatagatta tttttggcat
    21841 gattcttgta atgcctgcaa gagatttcat atttgggaga gcatcatgct ggaactactt
    21901 tttgtgattg gcttttttgt catgctgatg gtcaccggcg tttcgttgct gggcattatc
    21961 gccgcgctgg ttgtggcgac ggccattatg ttcctcggcg gtatgctggc attgatgatt
    22021 aagttgctgc cgtggttact cctggcgatt gcggtggtgt gggttattaa ggcgattaaa
    22081 gcaccaaaag tgccgaaata tcagcgttat gaccgctggc gttactaagg gattgtgcgg
    22141 atgatcacaa cctaaggttt tatccttaga acaaaatagg aattgataat caaatctgtc
    22201 actattgcgc ctctaacaga ttcatcgtgc tgtaccctac atacagccga actataaaaa
    22261 gaaagggctt cccagatgga agccctattt cttttatgga atcaacaggc tggaaccttg
    22321 cgtcgcccgg ctttccagaa tctcatgcgc acgctgtgca tccttaagcg gatatttctg
    22381 ctgctcggcg acatcgacct taatcacacc gctggcaatc aaagagaaca gttcattact
    22441 ggcctcggtt aattcctccc gtgtggtgat atagccttgt agggaagggc gtgtcacata
    22501 caacgagcct ttctgattga gaatgcctaa gttcacaccg gtaaccgcac ctgatgagtt
    22561 gccaaagctc accattaagc cgcggcgttg caggcaatcc agcgaccgtt cccaggtgtc
    22621 tctgcccacg gaatcgtaca ccacgcgcac tttcttgcca ccggtgatct cttttaaccg
    22681 ctcgaccaga ttctcttcac gatagttaat aacctgccat gcgcccgctt ttagcgcact
    22741 ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc gcgccaaggg cttttgccca
    22801 ctggcaggca atcaagccaa cgccgccagc cgctgcgtgg aacaggaact gctcatcggg
    22861 tttaatttca taggttttgc gcagcagata ataaaccgtt aagcctttca ggaaggatgc
    22921 cgcagcttgc tcaaaagaaa ttgccgcagg cagaatcgcc gctttatccg cattaatgtt
    22981 atgcacagag ctgtaagcgc ctaatgccga ttgtgcatag actacgcgat cgcctgcctt
    23041 aatatgcttt acaccactgc cgactttact cacgatgcct gccgcttcgg tgcctaatcc
    23101 gctgggtagc gatggcggcg ggtaaaggcc gctgcggata tacgtgtcga taaaattgat
    23161 gccgatggct ttattttcga cctggatttc attctccgcc ggatcggcag gagtgaactc
    23221 tacggcttga agtacttccg ggccaccgtg cttgtgaaat tcaattcgtg ttgccatgtg
    23281 tcctccagaa cgtatcgtca gggtctgctt catatggtaa agtttcgacc cattctttat
    23341 ctcggtaact ccattcacta tggcaggaaa taaacccttc aacaaacagc aggctgaacc
    23401 ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct ccgcactcga tcgaagcgga
    23461 gcagtcggtg ttgggcggtt taatgctgga taacgaacgc tgggatgatg tagctgagcg
    23521 tgtggtggca gatgattttt acacccgccc acaccgtcat atctttactg aaatggcgcg
    23581 tttgcaggaa agcggtagtc ctatcgatct gattaccctt gcggaatcgc tggaacgcca
    23641 ggggcaactc gatagcgtcg gtggttttgc ttatctggca gagctgtcaa aaaatacgcc
    23701 aagtgcggcg aacatcagtg cttatgctga catcgtgcgt gaacgtgccg ttgttcgcga
    23761 gatgatttcg gttgcgaatg agattgccga agccggtttt gatccgcagg ggcgcaccag
    23821 cgaagatctg ctggacctgg ctgaatcccg cgtctttaaa attgccgaaa gtcgtgcaaa
    23881 caaagacgaa gggccgaaga acatcgctga tgtgctcgac gccacggtgg cgcgtattga
    23941 gcagttgttt cagcagccac acgatggcgt taccggggta aacaccggtt atgacgatct
    24001 caacaaaaaa accgctggct tgcagccgtc ggatttgatc atcgtcgccg cgcgtccgtc
    24061 gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac gcggcgatgt tgcaggataa
    24121 accagtactt atcttctcgc tggagatgcc atcagaacag attatgatgc gttctctggc
    24181 gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg cagctcgatg atgaagactg
    24241 ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa cgcaatatct atatcgatga
    24301 ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgcg cgccgtattg cccgtgaaca
    24361 cggcggcatc gggcttatca tgatcgacta cctgcaactg atgcgcgtac cggcgctttc
    24421 cgataaccgt acgctggaaa ttgcagaaat ctcccgctcg ctgaaagcac tggcgaaaga
    24481 attgaacgtg ccggtggtgg cgctgtccca gttgaaccgt tctctggaac aacgtgccga
    24541 caaacgcccg gtcaactccg acctgcgtga atctggctct atcgagcagg atgcggactt
    24601 gatcatgttt atctatcgtg atgaggtgta tcacgaaaac agtgatttaa aaggcatcgc
    24661 ggaaattatt atcggtaaac aacgtaacgg cccaatcggg acggtacgcc tgacctttaa
    24721 cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag tacgacgacg aataataatt
    24781 attttatgaa ttaggtaatt aaagcaaaca cttatcaagg aacacaaatg caagcggcaa
    24841 ctgttgtgat taaccgccgc gctctgcgac acaacctgca acgtcttcgt gaactggctc
    24901 ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta tggtcacggt cttcttgaga
    24961 ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc ccgtctcgaa gaagctctgc
    25021 gactgcgtgc ggggggaatc accaaacctg tactgttact cgaaggcttt tttgatgcca
    25081 gagatctgcc gacgatttct gcgcaacatt ttcataccgc cgtgcataac gaagaacagc
    25141 tggctgcgct ggaagaggct agcctggacg agccggttac cgtctggatg aaactcgata
    25201 ccggtatgca ccgtctgggc gtaaggccgg aacaggctga ggcgttttat catcgcctga
    25261 cccagtgcaa aaacgttcgt cagccggtga atatcgtcag ccattttgcg cgcgcggatg
    25321 aaccaaaatg cggcgcaacc gagaaacaac tcgctatctt taataccttt tgtgaaggca
    25381 aaccaggtca acgttccatt gccgcgtcgg gtggcattct gctgtggcca cagtcgcatt
    25441 ttgactgggt gcgcccgggc atcattcttt atggcgtctc gccgctggaa gatcgttcca
    25501 ccggtgccga ttttggctgt cagccggtaa tgtcattgac ctccagcctg attgctgtgc
    25561 gtgagcataa agccggagag cctgtcggtt atggtggaac ctgggtaagc gaacgtgata
    25621 cccgccttgg cgtagtcgcg atgggctatg gcgatggtta tccgcgcgcc gcgccgtccg
    25681 gtacgccagt gctggtgaac ggtcgcgaag tgccgattgt cgggcgcgtc gcgatggata
    25741 tgatctgcgt agacttaggt ccacaggcgc aggacaaagc cggggacccg gtcattttat
    25801 ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac aaaagtgagc gcttacgaac
    25861 ttattacgcg cctgacttca agggtcgcga tgaaatacgt ggattaatcg ttctgtaata
    25921 tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc ggccaataaa atcttaccaa
    25981 ttcaataagt tgatattctt tcatgctctt ataaaggtcg tgcatctggc ggatgtacgt
    26041 ttgtcatgag tctcactctg ttgctaattg ccattcgccc ctgaacatcc tctcgatctt
    26101 cgccttcttc cggtttattg tgttttaacc acctgcccgt aaacctggag aaccatcgcg
    26161 tgtttcaaaa agttgacgcc tacgctggcg acccgattct tacgcttatg gagcgtttta
    26221 aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg tctgtactac aacgaagacg
    26281 gaattattcc acaactgaaa gccgtagcgg atgcggaagc gcgcctgaat gcgcagcctc
    26341 atggtgcttc gctttattta ccgatggaag ggcttaacag ctatcgccat gccattgcgc
    26401 cgctgctgtt tggtgcggac catccggtac tgcaacaaca gcgcgtagca accattcaaa
    26461 cccttggcgg ctcaggggca ttgaaagtgg gtgcagattt cctgaaacgc tacttcccgg
    26521 aatcaggcgt ctgggtcagc gatcctacct gggaaaacca cgtagcaata ttcgccgggg
    26581 ctggattcga agtgagcact tacccctggt atgacgaagc gactaacggc gtgcgcttta
    26641 atgacctgtt ggcgacgctg aaaacattac ctgcccgcag tattgtgttg ctgcatccat
    26701 gttgccacaa cccaacgggt gccgatctca ctaatgacca gtgggattcc gtgattgaaa
    26761 ttctcaaagc ccgcgagctt attccattcc tcgatattgc ctatcaagga tttggtgccg
    26821 gtatggaaga ggatgcctac gccattcgcg ccattgccag cgctggatta cccgctctgg
    26881 tgagcaattc gttctcgaaa attttctccc tttacggcga gcgcgtcggc ggactttctg
    26941 ttctgtgtga agatgccgaa gccgcaggcc gcgtactggg gcaattgaaa gcaacagttc
    27001 gccgcaacta ctccagcccg ccgaattttg gtgcgcaggt ggtggctgcg gtgctgaatg
    27061 acgaggcatt gaaagccagc tggctggcgg aagtagaaga gatgcgtact cgcattctgg
    27121 cgatgcgtca ggaactggtg aaggtattga gcacagagat accagaacgc aatttcgatt
    27181 atctgcttaa tcagcgcggt atgttcagtt ataccggttt aagtgccgct caggttgacc
    27241 gactacgtga agaatttggt gtctatctca tcgccagcgg tcgcatgtgt gtcgccgggt
    27301 taaatacggc aaatgtacaa cgtgtggcaa aggcgtttgc tgcggtgatg taatgcagga
    27361 aagcaggctg gagttatcca gcctgcagtg aaattaaact gtcgtcgctt tcactcttgc
    27421 tttatagatg atttttttga tgccatcgtt ctacgtgaga ggtaataaaa cgttgttagt
    27481 tctaacttgt taagtttatc ccaatgttct ggagtacttt tccctgtttt accgt
//
LOCUS       DAZKJR010000064        27418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKJR010000064 DAZKJR010000000
VERSION     DAZKJR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.064"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..418
                     /gene="cmtB"
                     /locus_tag="ACHOWS_002674"
     CDS             <1..418
                     /gene="cmtB"
                     /locus_tag="ACHOWS_002674"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFQ0048225.1"
                     /translation="SSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQAIKDSTINN
                     GPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIGLSAADADSH
                     IGAIQALSELLCEEEILNQLLTAASEKQLADIISRG"
     gene            446..1834
                     /gene="cmtA"
                     /locus_tag="ACHOWS_002675"
     CDS             446..1834
                     /gene="cmtA"
                     /locus_tag="ACHOWS_002675"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFQ0048226.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSVI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            1849..3126
                     /gene="yggP"
                     /locus_tag="ACHOWS_002676"
     CDS             1849..3126
                     /gene="yggP"
                     /locus_tag="ACHOWS_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFQ0048227.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTDKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3123..3438
                     /locus_tag="ACHOWS_002677"
                     /pseudo
     CDS             3123..3438
                     /locus_tag="ACHOWS_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            3460..3969
                     /gene="fumE"
                     /locus_tag="ACHOWS_002678"
     CDS             3460..3969
                     /gene="fumE"
                     /locus_tag="ACHOWS_002678"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFQ0048228.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSRMVKTALTLAV
                     TTTLKELTP"
     gene            3966..4679
                     /gene="yggC"
                     /locus_tag="ACHOWS_002679"
     CDS             3966..4679
                     /gene="yggC"
                     /locus_tag="ACHOWS_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFQ0048229.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLVNS
                     RQANLILEMTEEGRYHFKS"
     gene            4964..5983
                     /gene="epd"
                     /locus_tag="ACHOWS_002680"
     CDS             4964..5983
                     /gene="epd"
                     /locus_tag="ACHOWS_002680"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFQ0048230.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6033..7196
                     /gene="pgk"
                     /locus_tag="ACHOWS_002681"
     CDS             6033..7196
                     /gene="pgk"
                     /locus_tag="ACHOWS_002681"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0048231.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            7410..8489
                     /gene="fbaA"
                     /locus_tag="ACHOWS_002682"
     CDS             7410..8489
                     /gene="fbaA"
                     /locus_tag="ACHOWS_002682"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0048232.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            8680..9540
                     /gene="mscS"
                     /locus_tag="ACHOWS_002683"
     CDS             8680..9540
                     /gene="mscS"
                     /locus_tag="ACHOWS_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFQ0048233.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            9679..10314
                     /gene="argO"
                     /locus_tag="ACHOWS_002684"
     CDS             9679..10314
                     /gene="argO"
                     /locus_tag="ACHOWS_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFQ0048234.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIVHAQALLS"
     gene            10407..11147
                     /gene="yggE"
                     /locus_tag="ACHOWS_002685"
     CDS             10407..11147
                     /gene="yggE"
                     /locus_tag="ACHOWS_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFQ0048235.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            11315..12211
                     /gene="ygfI"
                     /locus_tag="ACHOWS_002686"
     CDS             11315..12211
                     /gene="ygfI"
                     /locus_tag="ACHOWS_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0048236.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(12208..13686)
                     /gene="scpC"
                     /locus_tag="ACHOWS_002687"
     CDS             complement(12208..13686)
                     /gene="scpC"
                     /locus_tag="ACHOWS_002687"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFQ0048237.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPNAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(13710..14495)
                     /gene="scpB"
                     /locus_tag="ACHOWS_002688"
     CDS             complement(13710..14495)
                     /gene="scpB"
                     /locus_tag="ACHOWS_002688"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFQ0048238.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(14506..15501)
                     /gene="meaB"
                     /locus_tag="ACHOWS_002689"
     CDS             complement(14506..15501)
                     /gene="meaB"
                     /locus_tag="ACHOWS_002689"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFQ0048239.1"
                     /translation="MINEAKLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNRLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(15494..17638)
                     /gene="scpA"
                     /locus_tag="ACHOWS_002690"
     CDS             complement(15494..17638)
                     /gene="scpA"
                     /locus_tag="ACHOWS_002690"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFQ0048240.1"
                     /translation="MSKVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLDGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSAAE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(17842..18735)
                     /gene="argP"
                     /locus_tag="ACHOWS_002691"
     CDS             complement(17842..18735)
                     /gene="argP"
                     /locus_tag="ACHOWS_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFQ0048241.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18876..19061
                     /locus_tag="ACHOWS_002692"
     CDS             18876..19061
                     /locus_tag="ACHOWS_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048242.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFASWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            19117..19776
                     /gene="rpiA"
                     /locus_tag="ACHOWS_002693"
     CDS             19117..19776
                     /gene="rpiA"
                     /locus_tag="ACHOWS_002693"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0048243.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20032..21264
                     /gene="serA"
                     /locus_tag="ACHOWS_002694"
     CDS             20032..21264
                     /gene="serA"
                     /locus_tag="ACHOWS_002694"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0048244.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLAEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21493..21552
                     /gene="ibsC"
                     /locus_tag="ACHOWS_002695"
     CDS             21493..21552
                     /gene="ibsC"
                     /locus_tag="ACHOWS_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFQ0048245.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(21653..22255)
                     /gene="fau"
                     /locus_tag="ACHOWS_002696"
     CDS             complement(21653..22255)
                     /gene="fau"
                     /locus_tag="ACHOWS_002696"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0048246.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTLPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(22276..22459)
                     /gene="ssrS"
                     /locus_tag="ACHOWS_002697"
     ncRNA           complement(22276..22459)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOWS_002697"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22501..22830)
                     /gene="zapA"
                     /locus_tag="ACHOWS_002698"
     CDS             complement(22501..22830)
                     /gene="zapA"
                     /locus_tag="ACHOWS_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0048247.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALFEQGRITE
                     KTNQNFE"
     gene            22998..23576
                     /gene="ygfB"
                     /locus_tag="ACHOWS_002699"
     CDS             22998..23576
                     /gene="ygfB"
                     /locus_tag="ACHOWS_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFQ0048248.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23602..24927
                     /gene="pepP"
                     /locus_tag="ACHOWS_002700"
     CDS             23602..24927
                     /gene="pepP"
                     /locus_tag="ACHOWS_002700"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFQ0048249.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24924..26102
                     /gene="ubiH"
                     /locus_tag="ACHOWS_002701"
                     /gene_synonym="visB"
     CDS             24924..26102
                     /gene="ubiH"
                     /locus_tag="ACHOWS_002701"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFQ0048250.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
                     "
     gene            26125..27327
                     /gene="ubiI"
                     /locus_tag="ACHOWS_002702"
                     /gene_synonym="visC"
     CDS             26125..27327
                     /gene="ubiI"
                     /locus_tag="ACHOWS_002702"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFQ0048251.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         6544 a         6734 c         7342 g         6798 t
ORIGIN      
        1 atcatcaatc tcagttatac attcagcaaa agattggcag gaagctatcg atttctcgat
       61 ggcatcattg ctggataaaa actatatcag cgagaattac attcaggcaa ttaaagattc
      121 caccattaac aatggccctt attatattct cgcaccaggc gtggcaatgc ctcatgctcg
      181 accggaatgt ggggcgctta aaaccgggat gtctttaaca ttacttaaac aaggcgttta
      241 ttttccgggg aatgacgaac cgataaaatt actcatcggg ctttctgctg ccgatgccga
      301 ttcgcacatt ggcgctattc aggcgttaag tgaattattg tgtgaagaag aaatactcaa
      361 ccaattatta acagcagcat cagaaaaaca attagcggac ataatcagcc gcggataatt
      421 actctctcct ctacaggata atattatgga aaacaagtct gctcgtgcaa aggtccaggc
      481 ttttgggggc tttttgactg caatggtcat ccccaatatc ggcgctttta tagcctgggg
      541 ttttataacg gcgttattta ttcccaccgg ctggctgcct aacgaacatt tcgccaaaat
      601 tgtcggcccg atgattacct atttattgcc cgtgatgatt ggttctaccg gtggtcatct
      661 ggtcggcggt aaacgcggcg cggtcatggg cggaataggt actattggtg tgatcgtcgg
      721 cgcagagatc ccgatgttcc ttggctcaat gattatgggg ccgctcggtg gtctggtgat
      781 taaatatatc gacaaggcgc tggaaaagcg gatccctgct ggttttgaga tggttatcaa
      841 taacttctca ttaggtattg cggggatgct cctttgtctg ctgggttttg aagttatcgg
      901 cccggcggtg ttaattgcca atactttcgt caaagagtgt attgaggcgt tggtacatgc
      961 gggttatctg cctttgctgt cagtcatcaa tgaaccggcg aaagtgcttt tcctcaataa
     1021 cgcgatagat cagggcgtct attacccgtt ggggatgcaa caggcttcag ttaacggtaa
     1081 atccatcttc tttatggtgg cgtccaaccc tgggcctggc ttaggactgt tgctggcgtt
     1141 tactttgttt ggtaaaggga tgagtaaacg ttctgcgccc ggggcgatga ttattcactt
     1201 cctcggtggg atccacgaac tgtatttccc gtatgtgctg atgaagccgc tgaccattat
     1261 tgccatgatt gcgggcggta tgtctggcac ctggatgttt aacttactgg acggtggtct
     1321 ggtggctggc ccaagtccgg ggtctatctt tgcttacctg gcactgacgc cgaaaggctc
     1381 gttcctggcg acaattgccg gtgttacggt aggtaccctg gtgtcctttg ctatcacttc
     1441 cgtaatactg aagatggaaa aaacggtgga aacggagagc gaagatgagt ttgctcagtc
     1501 agccaatgcg gttaaggcga tgaaacaaga aggtgcattc tcgttaagca gggttaagcg
     1561 tatcgccttt gtttgcgatg cggggatggg ctccagcgcg atgggcgcga ccaccttccg
     1621 taaacgcctg gaaaaggcgg ggctggcaat agaagtaaaa cattacgcga tagaaaacgt
     1681 gcctgcggat gcggatatcg ttgttactca tgccagtctg gaagggcgcg tgaaacgtgt
     1741 gacggataaa ccgctgatac tgattaataa ctatattggc gatccaaaac tcgacacttt
     1801 atttaatcaa ttaaccgccg aacataaaaa ctgattgccg aggtaaaaat gaaaaccaaa
     1861 gttgctgcta tttatggcaa gcgggatgtc cgtctgcgcg aatttgaact gccagaaatt
     1921 accgataatg aactgctggt gagtgtgatt tctgacagcg tgtgtttatc cacctggaaa
     1981 gcggcgctac tcggtagtga acataaacgc gtacccgacg atttagaaaa tcatccggtc
     2041 attaccgggc atgaatgcgc cggggttatt gtcgaagtgg gtaaaaatct caccgacaaa
     2101 tataaaaaag gccagcgttt tgtattgcaa ccggcaatgg ggttaccaag cggatattca
     2161 gcaggctaca gctacgaata ttttggcggt aacgccacct atatgattat tcctgaaata
     2221 gccattaacc tgggctgcgt attaccgtat cacggctctt attttgctgc ggcgtcgctg
     2281 gcagagccta tgtgctgcat tattggcgct tatcatgcca attatcacac cacgcaatat
     2341 gtttatgagc atcgcatggg cgttaaacct ggcggcaata ttgcgctgct ggcatgtgct
     2401 ggtccgatgg gcattggcgc tatcgattat gctattaacg gcggcataca accgtcgcgg
     2461 gtggtggtgg tcgatatcga cgacaaacgc ctggcgcagg tacaaaagct gctgccggtg
     2521 gaactggcgg ccagtaaagg cattgagctg gtgtatatga ataccaaagg gatgagcgat
     2581 ccggtccaga cgctgcgggc gctgacggat gatgccggat ttgatgacgt atttgtctat
     2641 gcagcggtgc cttcagtcgt cgagatggct gatgaattac tggcggaaga tggctgcctg
     2701 aacttctttg ccgggccgac ggataaaaac ttcaaagtgc cgtttaattt ctacaacgtc
     2761 cattacaaca gcacgcatgt cgtcggtacg tccggcggtt caacggacga catgaaagag
     2821 gcgattgcgc ttagcgccac agggcagttg cagccgtcgt ttatggtgac ccatatcggt
     2881 gggctggatg cggtgcctga taccgtgctc aatctgccgg atatccccgg cggtaaaaaa
     2941 ctcatttata acggcgtcac catgccgctc actgccattg ccgattttgc cgaaaaaggc
     3001 aaaaccgatc cgctgtttaa agagctggcg cggctggttg aggaaacgca cggcatctgg
     3061 aatgagcagg ccgagaaata tctgctggcg caatttggcg ttgatatcgg ggaggccgcg
     3121 caatgatgtc cctggcgtgg ccattatttc gcgttacgga acaggcagcg ttggctgcct
     3181 ggccgcaaac cggatgtggc gacaaaaata aaattgatgg cctggcggtc accgcgatgc
     3241 gccaggcatt aaacgacgtc gcttttcgtg ggcgagtggt tatcggcgag ggggaacgat
     3301 atcctcttta gcgctacggg cgtgacgggc ggcgagttgg tgaacggtat ccaacagacg
     3361 gcgaatgggg tgcggacgca gacattactg atcggcggcg cggaccaaac gtgtaatata
     3421 atagactccc tgcattgatg gtgatctatc ggaagaccaa tggcgacgct gacagaagat
     3481 gatgtgcttg agcaactgga tgcacaggac aatttatttt catttatgaa aactgcgcat
     3541 tctattttgc tccaggggat acgccagttt ctgccgtcgc tgtttgtcga taacgatgaa
     3601 gagatcgtcg aatatgcagt gaagccgtta ctcgcccaaa gcggcccgct tgacgatatt
     3661 gatgttgcgc tgcgtttgat ttatgcgctg gggaaaatgg ataaatggct gtacgccgat
     3721 atcacgcatt tttcccagtt ttggcattac ctgaacgaac aggatgaaac gcccggattt
     3781 gccgatgaca tgacctggga ttttatcagc aacgtcaata gcataacctg caatgcgacg
     3841 ctctatgacg cgttgaaagc gatgaagttc gccgatttcg ccgtctggtc agaggcgcgt
     3901 tttagcagga tggtcaaaac ggcgctgacg ctggcagtaa cgaccacctt aaaggaatta
     3961 acgccgtgaa aattgaatta acggtgaatg ggctgaagat acaggcacag taccagaacg
     4021 aagagattga aaatgtacat aaaccgttgc tgcacatgtt ggcggcctta cagacggtaa
     4081 atccgcagcg gcgtacggtg gtttttcttt gcgctccgcc ggggacaggt aaatctaccc
     4141 taaccacctt ctgggaatat ctcgcacaac aagacccgga actgcccgct attcagacac
     4201 tgccgatgga tggttttcac cattacaata gctggctgga tgcgcatcaa ttgcgcccct
     4261 tcaaaggcgc accagagaca ttcaacgttg cgaaactggc ggaaaatctg tgccgggttg
     4321 tggaagggga ttgtacgtgg ccgcagtacg atcgacaaaa gcatgatcct gttgaagatg
     4381 cgttgcacgt taccgcacca ctcgtcatcg tcgaaggaaa ctggctatta ctggatgatg
     4441 aaaagtggca ggagttggca tctttctgcg atttttctat ttttattcat gcacctgctc
     4501 aaattctgcg ggagcgcttg attagtcgta aaattgcggg aggattatcg cggcaagaag
     4561 ccgaagcttt ttatgcccga accgatggtc ccaatgtcga gcgtgtgctg gtgaatagtc
     4621 gtcaggccaa tctgatatta gagatgaccg aagaaggcag atatcacttc aaaagctgac
     4681 aatttattcc agttattgca ggggtattat tacgccagcc tggaataaag ccccctacaa
     4741 aaggggataa aagtgtgatg taagtcagat aaatgtcttc ttcggctgga caatcattcc
     4801 ttttattcca cgtttcgctt atcctagctg aagcgtttca gtcgattaaa tgttcgacaa
     4861 ttaaccaatc agtcgcagtt tgcgacaggt aaggtttccc cggacgattt gctggattac
     4921 tctgtctggc aaatttgctc agggaaaacc ttgcaggaga tctatgaccg tacgcgtagc
     4981 gataaatggc ttcggtcgca tcgggcgtaa tgtggttcgt gctttgtatg aatccggacg
     5041 ccgggcggaa attaccgtgg tggcaatcaa cgaactggcg gatgctgcgg gcatggcgca
     5101 tttgttgaaa tatgacacca gccatggccg ttttgcatgg gaagtacgac aggaacgcga
     5161 tcaacttttt gttggtgatg acgccatccg tgtattgcat gaacgttcac tgcaatcgct
     5221 cccctggcgt gaacttggcg ttgatgtagt cctcgactgc accggcgtat atggctcccg
     5281 cgagcatggc gaagcgcata ttgccgccgg ggccaaaaaa gtgctctttt cacatccagg
     5341 cagtaacgat ctcgacgcga ccgttgttta cggcgtcaat caggatcaac ttcgtgcgga
     5401 acaccgcatc gtttctaacg cttcctgtac cacgaattgc ataattcccg tcatcaaatt
     5461 gttagatgat gcgtacggta ttgagtccgg cactgtgacc acaattcact ccgccatgca
     5521 cgatcaacag gttattgatg cataccatcc tgacctgcgt cgcacccggg ctgccagcca
     5581 gtcgatcatt ccggtcgata ctaaactggc cgccggtatc acacgatttt ttccgcaatt
     5641 taacgatcgc tttgaagcga ttgcggtacg tgtgccaacc ataaatgtga cggcaatcga
     5701 tttaagcgtg acggtgaaga aacctgtaaa agccaatgaa gtcaacctgt tgctgcaaaa
     5761 agcagcacaa ggtgcatttc atggtatagt tgactatacg gaattgccgt tggtctctgt
     5821 agattttaac cacgatccgc acagtgccat tgtcgatggc acccaaaccc gggttagtgg
     5881 cgcacacctg atcaaaacgt tggtctggtg cgataacgaa tggggctttg ctaaccgaat
     5941 gctcgacacg acgttagcta tggctactgt tgctttcagg taagacgcaa gcagcgtctg
     6001 caaaactttt agaatcaacg agaggattca ccatgtctgt aattaagatg accgatctgg
     6061 atcttgctgg gaaacgtgta tttatccgtg cggatctgaa cgtaccagta aaagacggga
     6121 aagtaaccag cgacgcgcgt atccgtgctt ctctgccgac catcgaactt gccctgaaac
     6181 aaggcgcaaa agtgatggta acttcccacc tgggtcgtcc taccgaaggc gagtacaacg
     6241 aagaattctc tctgctgccg gttgttaact acctgaaaga caaactgtct aacccggttc
     6301 gtctggttaa agattacctc gacggcgttg acgttgctga aggtgaactg gttgttctgg
     6361 aaaacgttcg cttcaacaaa ggcgagaaga aagacgacga aaccctgtcc aaaaaatacg
     6421 ctgcactgtg tgacgtgttc gtaatggacg cattcggtac tgctcaccgc gcgcaggctt
     6481 ctactcacgg tatcggtaaa ttcgctgacg ttgcgtgcgc aggcccgctg ctggcagctg
     6541 aactggacgc gctgggtaaa gcactgaaag aacctgctcg cccgatggtg gctatcgttg
     6601 gtggttctaa agtatctacc aaactgaccg ttctggactc cctgtctaaa atcgctgacc
     6661 agctgattgt tggtggtggt atcgctaaca cctttatcgc ggcacaaggc cacgatgtgg
     6721 gtaaatccct gtacgaagct gatctggttg acgaagcaaa acgtctgctg accacctgca
     6781 acatcccggt tccgtctgat gttcgtgtag caactgagtt ctctgaaact gcaccggcta
     6841 ccctgaaatc tgttaacgat gtgaaagctg acgagcagat cctggatatc ggtgatgctt
     6901 ccgctcagga actggctgaa atcctgaaga atgcgaaaac cattttgtgg aacggtccgg
     6961 ttggcgtgtt cgaattcccg aacttccgca aaggtactga aatcgtggct aatgctatcg
     7021 cagacagcga agcgttctcc atcgctggcg gcggcgacac tctggcagca atcgacctgt
     7081 tcggcattgc tgacaaaatc tcctacatct ccactggcgg cggtgcattc ctcgaattcg
     7141 tggaaggtaa agtactgcct gcagtagcga tgctcgaaga gcgcgctaag aagtaaaaaa
     7201 tcacagggca gggaaacctg cccttgttgc agcgcgcttt taaagcacgc actttttcat
     7261 actctaaata attcgagttg caggaaggcg acaagagagt gaatcccatg agcttacata
     7321 agtaagtgac tggggtgagc gaacgcagac gcagcacatg caacttgaag tatgacgagt
     7381 ataaggcccg acgatacagg acaagagaca tgtctaagat ttttgatttc gtaaaacctg
     7441 gcgtaatcac tggtgatgac gtacagaaag ttttccaggt agcaaaagaa aacaacttcg
     7501 cactgccagc agtaaactgc gtcggtactg actccatcaa cgccgtgctg gaaaccgctg
     7561 ctaaagttaa agcgccggtt atcgttcagt tctccaacgg tggtgcttcc tttatcgctg
     7621 gtaaaggcgt gaaatctgac gttccgcagg gtgctgctat cctgggcgcg atctctggtg
     7681 cgcatcacgt tcaccagatg gctgaacatt atggtgttcc ggttatcctg cacaccgacc
     7741 actgcgcgaa gaaactgctg ccgtggatcg acggtctgct ggacgcgggt gaaaaacact
     7801 tcgcagctac cggcaagccg ctgttctctt ctcacatgat cgacctgtct gaagaatctc
     7861 tgcaagagaa catcgaaatc tgctctaaat acctggagcg catgtccaaa atcggcatga
     7921 ctctggaaat cgaactgggt tgcaccggtg gtgaagaaga cggcgtggac aacagccaca
     7981 tggacgcttc tgcactgtac acccagccgg aagacgttga ttatgcgtat accgagctga
     8041 gcaaaatcag cccgcgtttc accatcgcag cgtccttcgg taacgtacac ggtgtttaca
     8101 agccgggtaa cgtggttctg actccgacca tcctgcgcga ctctcaggaa tatgtttcta
     8161 agaaacacaa cctgccgcac aacagcctga acttcgtatt ccacggtggt tccggttcta
     8221 ctgctcagga aatcaaagac tccgtaagct acggcgtagt gaaaatgaac atcgataccg
     8281 atacccaatg ggcaacctgg gaaggcgttc tgaactacta caaagcgaac gaagcttatt
     8341 tgcagggtca gctgggtaac ccgaaaggcg aagatcagcc gaacaagaaa tactacgatc
     8401 cgcgcgtatg gctgcgtgcc ggtcagactt cgatgatcgc tcgtctggaa aaagcattcc
     8461 aggaactgaa cgcgatcgac gttctgtaag tcattccttt ctgcttatct caaggcccgc
     8521 tctgcgggtc tttttttcgc caaaagcaag gtgatgcaga tgagaaatct gtgatctatt
     8581 tggcaaaatt atgctttatt gtttaccctt gtcagactgc ccgccataag gcggcggagt
     8641 gtatttctcc attttgagtc agttgaaaag gaatattgaa tggaagattt gaatgttgtc
     8701 gatagcataa acggcgcggg aagctggctg gtggctaacc aggcgctgct gctaagttat
     8761 gcagtaaaca tcgtggcggc actcgcgatc atcattgttg gtttgattat cgcgcggatg
     8821 atttccaacg cggtgaatcg cctgatgatc tcccgtaaaa tcgatgccac tgttgctgat
     8881 tttctctctg cattagtccg ttacggtatt atcgccttta cgctaatcgc tgcactgggg
     8941 cgcgtgggcg tacaaacagc gtcagtcatt gctgtactcg gtgccgcagg cttagcggtt
     9001 ggtctggctt tgcaggggtc actgtctaac ctggccgctg gcgtgttact tgttatgttc
     9061 cgcccgttcc gtgccggaga atatgttgat ctcggcggcg tagccggtac tgtgctgagt
     9121 gtgcagattt tctccaccac catgcgtact gcagacggta aaattatcgt tattccgaac
     9181 ggtaaaatta ttgccggaaa tattattaac ttctctcgcg agccagttcg ccgtaacgaa
     9241 tttattattg gcgtggcgta tgattccgat atcgatcagg ttaagcagat cctgaccgat
     9301 attatccagt ctgaagatcg cattttgaaa gatcgcgaaa tgactgtgcg cctgaacgaa
     9361 cttggtgcat cgtcgattaa tttcgtggtc cgcgtctgga gcaacagcgg cgatctgcaa
     9421 aacgtgtact gggatgtgct ggagcgtatt aaacgtgaat ttgatgccgc cggtatcagc
     9481 ttcccgtacc cgcaaatgga tgtgaacttt aagcgggtga aagaagacaa agctgcgtaa
     9541 tcaacgctga ggccagataa tactctctgg cctctctctt attagttttt ctgattgcca
     9601 attaatatta tcaatttccg ctaataacaa tcccgcgata tagtctctgc atcagatact
     9661 taattcggaa tatccaacgt gttttcttat tactttcaag gtcttgcact tggggcggct
     9721 atgatcctac cgctcggtcc acaaaatgct tttgtgatga atcagggcat tcgtcgtcag
     9781 taccacatta tgattgcctt actttgcgct atcagcgatt tggtcctgat ttgcgccggg
     9841 atttttggtg gcagcgcgtt attgatgcag tcgccgtggt tgctggcgct ggtcacctgg
     9901 ggcggcgtag ccttcttgct gtggtatggt tttggcgctt ttaaaacagc gatgagcagt
     9961 aatattgagt tagccagtgc cgaagtcctg aagcaaggca gatggaaaat tatcgccacc
    10021 atgctggcag tgacctggct gaatccgcat gtttacctgg atacttttgt tgtactgggc
    10081 agtcttggcg ggcaacttga tgtggaacca aaacgttggt ttgcgctcgg gacaattagc
    10141 gcctctttcc tgtggttctt tggtctggct attctcgcag cctggctggc accgcgcctg
    10201 cgcacggcaa aatcacagcg cattatcaat ctggtagtgg gatgtgttat gtggtttatt
    10261 gccttgcagc tggcgagaga cggtattgtt catgcacaag ccttgctcag ttaggcgcta
    10321 tctgatggaa aaataaaaca gaggcgctaa gcttgcctcc agaggtcctg aattttaggc
    10381 aagtcaatta acacggagag actaacgtga agttcaaagt tatcgccctg gcggcattaa
    10441 tgggtattag cgggatggca gcgcaggcta acgaattgcc ggatggaccg catattgtca
    10501 cctccggtac ggcaagcgtg gatgcggtgc cagacattgc cactcttgcg attgaagtca
    10561 acgtggccgc gaaggatgcc gctactgcca agaaacaggc ggatgagcgc gtcgcacaat
    10621 acatttcctt ccttgaactc aatcagatcg cgaaaaaaga tatcagctca gcgaacttac
    10681 gcacccagcc agattatgat tatcaggatg gtaaaagtat cctgaaaggc taccgtgctg
    10741 tgagaacggt ggaagtcacg ctccgtcagt tagacaaact gaattccttg ctggatggcg
    10801 cgctgaaggc gggtcttaac gaaattcgtt ctgtatcgct gggcgtggcg cagccggatg
    10861 cctataaaga caaagcgcgt aaggcagcga ttgataacgc gattcatcag gcgcaggaac
    10921 tggcgaacgg ctttcatcgt aaactggggc cggtatatag tgtgcgctac catgtttcca
    10981 actatcagcc cagcccaatg gtgcggatga tgaaagccga tgccgcgccg gtgtccgccc
    11041 aggaaactta cgagcaggcc gctattcagt ttgatgatca ggtcgatgtg gtcttccagt
    11101 tagaacctgt ggatcaacaa cccgctaaaa cacctgcagc acaataatat ttgtagcccg
    11161 acgtattcgt cgggctaatg cctttacccg atattgcctc ctttttctgt aattttctgt
    11221 aaaaccatga cgtaatcacc tatctttgct cgcggtttca tcaaggatag gctgactgaa
    11281 acggttaagc ggacaaggat gcgggggagg gggtatggac atttttattt cgaaaaagat
    11341 gcgcaatttc attttattag cgcaaaccaa taatattgcc cgggcggcag aaaaaatcca
    11401 tatgacagct tcgccatttg gtaaaagcat tgccgcgctg gaagaacaaa ttggctatac
    11461 gctatttacc cgcaaagata ataatatcag cctcaataaa gccggacagg aactgtatca
    11521 aaaactgttc ccggtttatc aacggctttc tgcgattgat aatgaaatcc ataactcagg
    11581 gcgtcgttca cgagaaattg tgattggcat agataatact tatccgacaa ttattttcga
    11641 tcagttgatt agtctcggag ataagtatga aggcgtgacc gcccagccag ttgagttcag
    11701 cgaaaatggc gttatcgata atctttttga ccgtcagtta gattttatta tctccccaca
    11761 gcatgtatct gcacgagttc aggaactgga aaacctgact atcagtgaac taccgccgct
    11821 gcgattgggc tttctcgttt ctcgtcgtta tgaagagcga caagagcaag agttgcttca
    11881 ggagctaccc tggctacaaa tgcgctttca gaaccgggct aacttcgaag caatgattga
    11941 tgcgaatatg cgtccttgtg ggatcaatcc caccattatt tatcgtccct acagttttat
    12001 ggccaaaatc agcgccgtgg agcgcggaca ttttctgacg gtgatcccgc attttgcctg
    12061 gcgactggtg aatccggcaa cgctgaaata ttttgatgcg ccgcacaggc cgatgtatat
    12121 gcaggaatat ctttattcaa tcagaaatca tcgttatacc gccacgatgc ttcagcatat
    12181 tgctgaagat cgtgacggga cgagtcatta acccagcatc gagccggttg caattaaatt
    12241 acggtgtaag tcgaagacgt ggctaagatc gtggtgaata tgtccgccgg gcgcattttc
    12301 cagatagcga tgcagataat cccgatacat aggatgtgca caattatcaa tgatagtgcg
    12361 ggcgcgttga agcggggaaa gaccacgcag atcggcgatg ccttgttcgg tgatgatcac
    12421 tttgacgctg tgttcgctgt gatcaacatg gctgcacatt ggcacgacgg ttgagatctt
    12481 gccttcttta gcaatcgacg gggccataaa gatcgacaga tacgcgttgc gttcaaaatc
    12541 accgctgcca ccgatgccgt tcatcagatc gaccccggct acgtgcgttg agttggcatg
    12601 cccgtaaata tcaaactcca ggccgacgtt cagagcgatg acgcccagac gacggatgat
    12661 ttccgggtta ttggaaatct cctgcggacg caacacaatg cggctggcaa agtaatccat
    12721 attgtcgtaa atcttgcgca gggaatcggc cgagatagtc aggctggagg cgctggcccc
    12781 gctgattttg cccgtttcca gtaaatgcac caccgattcc tgtagcactt ccgaatacat
    12841 cataaacgga ggaatttccg gattttcccc cagacgcgcc attaccgcat tattgatgtt
    12901 gcctacgcca ctttgcagcg gcagaaattc cggcggaata cgcccatgcg ccatttcctg
    12961 caacaagaac gtgaccacgt tatcggcaat ctgctggcac atgggatttt gcttatccag
    13021 catattaccg gcgtcaggca agttggtttc cacgacggcg acaatctttt tcggatcgat
    13081 ttgcacatag cgggtaccga cgcgatccat tgcatggaag atcgacacgc tattgcgccg
    13141 cggtggcgcg ccaggaatca caatatctgc cagttctgca acgcgcggat cgtgatagtg
    13201 attgagttca atgatcactt tcttcgcccg cagcagccag gtcggcgcat taccgatccc
    13261 gctggttaac cagactcgac catccggtgc cagtgccgat gcttcaatga cggcaacatc
    13321 aatgtcgccg aagaaaccgt aattgaccat ttgcgccact tcgctcaaat gcaggtcaac
    13381 gaaactcacc gcgccctgat tgatcttttt acgtaaaccg gacgatgttt gatatggcgc
    13441 acgccaggaa acagcatcgg cgtcagaaag tacatcgtca gcggcggcgc tgattgacgc
    13501 ccccgtcagc aggcgaattt gatacggctt tttggcctca tgctgttcgt tagctctgcg
    13561 ggcaatcgcg ttgggtaagg ctttcggcga acccgctggg gtaaagccgc taaatgccac
    13621 catgtcgtta tgctggataa tttctgccgc ttcatcggcg gtcatccttg tccactgagt
    13681 ttccatttta actccttcgt tcgcagggat taatgaccaa cgaaattagg tttacgtttt
    13741 tcgaggaaag cgttcatccc ttcctggtaa tcttcgctgt cataaaccgc gcggcgcatc
    13801 ccctgaatac gttcaaattc atcggagttc atggtgtgcg cttcgcccag tacacgcagc
    13861 tcttctttga taacggcaat ggctaacggc gctttctcag agatgtggtg cgccatttgt
    13921 aaggtgaaat cttccagttc ttccacttcc acaacatggt tgaggatgcc gacagccagc
    13981 gcgcgctggg ctgtgattgg cgaagcggta aaaatcagct ctttgacaat gtggaagccc
    14041 gcgtcgcggg tcaggttgtg aatgccgacc aggttatacg ggacgccgag gtttacaggt
    14101 gtcattgaga aggttgaggt actggcggcg atgatcagat cggaactcat gatcatttca
    14161 aatgcgccac cccagacact accttccacc atcgaaatga tcggtttcgg gaatttttgg
    14221 atcatgcggg tgatttgacg caacggatca tcataggaga gcggatcgcg accgccagac
    14281 ggcagttcgt gaatatcgtg acctgcggag aagactttgg aaccactcgg cgcgcgcaaa
    14341 atgatacagc gaatttccgg ccggttgaga tcgcttaatg cctgcataag atcatcaata
    14401 aagactttac ttaaggcatt aagtttgcgg ccatagttaa actcaatgac cgccactttg
    14461 ttgatagtga caacgttcac atactgataa gacataaaaa ttcctttaat caaaatattg
    14521 cgtctggata aattcactga gctgccgcag gccggtgcgc ggtgagagcg tattgttttt
    14581 gactgctaaa agcgtctggc ggtaatagcg atcgaaatct tcattcgcga acaggcgatt
    14641 cagtacttct tcttcggtct gcttacgcag ccattccacc gattgttgtt gccgcacttg
    14701 ttgtaatcga ccactggcag ttagcgcggt tttgaagtcg atgatggcgt gccagatctc
    14761 atcgattcca cgtttttcca gtgcgctaca agtcagaacc cgtggctgcc attcgtcgta
    14821 tttacgtcgc agaatatgca gggcactctc gtacatatgc cgggcaatgg cgacattggt
    14881 atggttatcg ccatcgtctt tgttgataac gatcagatct gccacttcca tcagcccctt
    14941 tttaatgccc tgcagatcat cgccgccacc ggcaatttgc aacgagataa aacagtccac
    15001 catgcgggcg acttccgttt ctgactgccc gacgccaacc gtttcgacaa tcactacgtc
    15061 ataacccgcc gcttcgcata acagcattaa ttcccgcgct cgctgactgg caccgcccag
    15121 atggccggag gagggtaccg ggcgaataaa cgccgcctcg gcacgcgcaa ggtcattcat
    15181 gcgggtttta tccccgagaa tgctaccgcc agtgaccggg ctgctgggat cgaccgcaat
    15241 aaccgcaacc tttaatccct cacgaatcaa caacatgcca aaggcttcaa gaaaggtact
    15301 tttccccgcg ccgggtgtgc cggtaacgcc cagtcgcagg gcgttaccac agaacggcat
    15361 aatggcgtca agcagctgtg tacttagcgc ctgatgacgc gggtgacggc tttccaccaa
    15421 cgtcatggcc tgggcgagcg tggcacgctc accctgacgt aagcggcgaa tactttctgc
    15481 tagcttggct tcattaatca tgatgctggc ttatcagatt cagtacgtcg cgcacgctgt
    15541 cgagcatagg tgtacctgga ccataaatcg ccgccacgcc gcgctcttgc aggaaggcgt
    15601 aatcctgcgg cggaatgacg ccacccgcga ccacgcagat atctttgcgt ccccattttt
    15661 tcagcgcttc gaccagttcc gggatcagcg ttttatgacc ggcagccagt gaggatgcgc
    15721 ccactacgtg aacgtcgttt tctacggcca ggcgggcgat ctcttcaggt gtagagaaca
    15781 tcgggcttaa atctacgtcg aaaccgagat cggaataggc gctggcgatc actttcgcgc
    15841 cgcgatcgtg cccatcctgg cccattttgg cgatcagaat gcgcgggcga cgaccattgt
    15901 cggcaaggaa ctgctccgtt tgcgcaacaa tggcatcgaa ctcggcggcc gatttctcag
    15961 actgatgata gctttgcgca atcacgccgg taacacactg gcttggcacc agataacggt
    16021 cgaaagcggc ttccagcgca tcggaaattt cacccagggt ggcgcgaacg cgagcagcat
    16081 taacagcggc agccagcagg ttttcgttat gctgtgcggc gtgagtcagg gcgttcaacg
    16141 cggcggttac ggcggcatca tcacgggtgg cgcgaatgcg ttccagcgaa gcaatttgct
    16201 cgttacgcac catcacgttg tcgatctcaa gtacatcggt ttcgtcttcg tgatccagtt
    16261 tgtacttgtt gacaccaacg atgacacgct tgccctggtc gatcagcgac tgttcgcgcg
    16321 ctgaggcctc ttcgatcatt cgttttggca gacctgcttc gatcgctttc gccatgccac
    16381 cggcttcgtc gatctgttgg ataatagctc tggcttgttt gacgatttga tcggtcagcg
    16441 actcaatgta ataggatccg gccagtggat cgacggtgcg gcagagttct gattcttcct
    16501 gaatgatgat ctgggtattg cgggcaatgc gtgctgagaa atcggtaggc aaaccaagcg
    16561 cttcatcaaa ggcgttggta tgcagtgact gagtaccgtc cagcgttgca gccagtgctt
    16621 caatggtggt gcggataacg ttgttatacg gatcctgttc agtcaggctc cagcctgagg
    16681 tctggcagtg ggtacgcagc gccagtgatt tcggatcctg tgcgccaaat ccactgaccg
    16741 cttcgctcca taaataacgt gccgcacgca acatggcgac gttcataaac agatccatgc
    16801 caatgccgaa gaagaacgac agacgaggag cgaagtcatc aattttcagc ccggcggaga
    16861 ttgctgcttt gatgtactca atcccatcgg cgagcgtaaa tgctacctgc tgcacgcagt
    16921 tggcacccgc ttcacccatg tggtaaccgc tgatactgat ggtattaaat cgcggcatgt
    16981 tgccggaaca ccaggcgatg atgtctgcga taatacgcat tgacggtttt ggcgggtaaa
    17041 tataggtgtt gcggcaaaga tactctttga ggatatcgtt ttgaatggtg ccggtcagtt
    17101 tatcgggtgt aacaccttgc tcttctgcgg cgacaatata aaacgccagt actggtagta
    17161 ctgcgccatt catggtcatc gaaaccgaca ttttatccag cgggatctgg tcgaacagga
    17221 ctttcatatc ttccacggtg tcgatagcaa cgcccgcttt gccgacgtcg cccgccacgc
    17281 gtgggttatc ggagtcgtag cctcggtggg tggcaaggtc aaacgcaacg gaaagacctt
    17341 tttgcccggc ggccaggtta cggcgataaa aagcgttgga ctcttttgct gttgaaaaac
    17401 cagcatactg acggatggtc cacggttggg cggtatacat agtggcacgc gggccacgaa
    17461 cgtagggagg caaaccagga agggtacctg tcacctccag attatcgaga tcggcttcgg
    17521 tatacagcgg cttgatggcg atcccttccg cggtttgctg aaccagcgag tcgacagttt
    17581 tctcccgacg gctcaattcc ttgttggcaa gctgttgcca ctcctgcacc ttagacatta
    17641 tttgctccgt aaaacgcaat caaaatgagg gtatttgcgc ttacggtgag ctttttacgt
    17701 gggtttgtcg ccagcaaaaa aaatgcattc agacgtcgtt ttttgccgcc tgagatttgt
    17761 ctattttgtg atgtgattca aggaatacaa aaagaagtga ggataacgcc tgatatgtgc
    17821 tatcaggcat tatttgatgg attaatcctg acgaaggact ttgtgaccat aatcgagcaa
    17881 cgcatcagtg actttacgca tcatgcggct ttcaggagca aagcggtgcc agtagagcat
    17941 ccgtcgttga aatagccctg gcgttaagtc aatcagttca ccgctggcca gctctttctc
    18001 gatttgcagg tgcgggatca tacagcaggt ggtgccctga cgagcaagtt gcacgaacgc
    18061 ttccgaagaa ttaacgatat ggcagggtac gctgccagga ggcagatcga agttttgctg
    18121 caaaaaggcc tggtgcatat cgtcaagatg gtcaaaagcg accacaggtg ctttcaataa
    18181 tgccgaacgc gttacgccgt tagggaaata tttttcggca aacggttttg agctgacgaa
    18241 cagatagtcg agcgcaccga gcttatcgac aaggcaactc ggcagcgcct gatgttgaat
    18301 actcaccgcg ccgaccactt cgccgcggcg cagacgttcc tgagtgcggg tttcatcttc
    18361 tacctgcaag ttgaggcgga taggcgaatc agctaacaca ggagccagcg ccggaagcaa
    18421 ccacgtcgcc agactgtcag cgttgaccgc cagtgaaagc agcagcggag ttgaaccggt
    18481 ttgttcatcg cccagccact cttcttccag taactccacc tggcgtagca gtgccagcag
    18541 tttttgccct tgttctgtcg ggcgcggtgg tacggtacgc accaacagcg gctgcccgaa
    18601 catattttcc agttgcttaa tgcgctgtga gacggctgat tgtgtaatgc acagcttttg
    18661 tgccgcgcgc tcaaatcctc gttcacgtat caccgcatcc agtgcctgta atgttctgta
    18721 gtccgggcgt ttcattgctg ttgtctgctc ctggtaaatg ctttttcctg cactatgaca
    18781 caattttgtg tcaggttgca tataccgaag tcgttggcgg gtaacatttg tatcagtggt
    18841 taacgcgcag cttaaaagat cagggatttg cgctaatgca tttcgctcag cgagttcgcg
    18901 ccctggttgt gttgaatggc gtggcgttat tgcctctgtt cgcatcgtgg agcggcatac
    18961 ccagaccgtt gtatgcttta tttgcggggc gaaaggggat gcccgccatt gcgcgatatt
    19021 ttatggatga gttaaccacc cggcttgcca acggggtctg aatcgctttt tttgtatata
    19081 atgcgtgtga aatttcatac cacaggcgaa acgatcatga cgcaggatga attgaaaaaa
    19141 gcagtaggat gggcggcact tcagtatgtt cagcccggca ccattgttgg tgtaggtaca
    19201 ggttccaccg ccgcacactt tattgacgcg ctcggcacga tgaaaggcca gattgaaggg
    19261 gccgtttcca gttcagatgc ttccactgaa aaactgaaaa gcctcggcat tcacgttttt
    19321 gatctcaacg aagtcgacag ccttggcatc tacgttgatg gcgcagatga aatcaacggc
    19381 cacatgcaaa tgatcaaagg cggcggcgcg gcgctgaccc gtgaaaaaat cattgcttcg
    19441 gttgcggaaa aatttatctg tattgccgac gcctccaagc aggttgatat tctgggcaaa
    19501 ttcccgctgc cagtagaagt tatcccgatg gcacgtagtg cagtggcgcg ccagctggtg
    19561 aaactgggcg gtcgtccgga ataccgtcag ggcgtggtga ccgataacgg caacgtgatc
    19621 ctcgacgtcc acggcatgga aatccttgac ccgatagcga tggaaaacgc cataaatgcg
    19681 attcctggcg tggtgactgt tggcttgttt gctaaccgtg gcgcggacgt tgcgctgatt
    19741 ggcacacctg acggtgtcaa aaccattgtg aaatgatctg acgggggaac ctcccccgtt
    19801 aaaaaaattc tcttcattaa atttggtgac atgtgtcacg tttttacctg gctattgtcg
    19861 attgctctaa ataaaccctc taaaccagca tattcatcca agaaatacct ttgcgtgata
    19921 tttcctcaat atcgtgacgc aaacgttcat attgccgcaa tattattttt tgatatgttg
    19981 aaaggcggat gcaaatccgc acacaacatt tcaaaagaca ggattgggta aatggcaaag
    20041 gtatcgctgg agaaagacaa gattaagttt ctgctggtag aaggcgtgca ccaaaaggcg
    20101 ctggaaagcc ttcgtgcagc gggttacacc aacatcgaat ttcacaaagg cgcgctggat
    20161 gatgaacaat taaaagaatc catccgcgat gcccacttca tcggcctgcg atcccgtacc
    20221 catctggctg aagacgtgat caacgccgca gaaaaactgg tcgctattgg ctgtttctgt
    20281 atcggaacaa accaggttga tctggatgcg gcggcaaaac gcgggatccc ggtatttaac
    20341 gcaccgttct caaatacgcg ctctgttgcg gagctggtga ttggcgaact gctgctgcta
    20401 ttgcgcggcg tgccggaagc caatgctaaa gcgcatcgtg gcgtgtggaa caaactggcg
    20461 gcgggttctt ttgaagcgcg cggcaaaaag ctgggtatca tcggctacgg tcatattggt
    20521 acgcaattgg gcattctggc tgaatcgctg ggaatgtatg tttactttta tgatattgaa
    20581 aacaaactgc cgctgggcaa cgccactcag gtacagcatc tttctgacct gctgaatatg
    20641 agcgatgtgg tgagtctgca tgtaccagag aatccgtcca ccaaaaatat gatgggcgcg
    20701 aaagagattt cgctaatgaa acccggctcg ctgctgatta atgcttcgcg cggtactgtg
    20761 gtggatattc cagcgctgtg tgacgcgctg gcgagcaaac atctggcagg ggcggcaatc
    20821 gacgtattcc cgacggaacc ggcgaccaat agcgatccat ttacctctcc gctgtgtgaa
    20881 ttcgacaatg tccttctgac gccacacatt ggtggttcga ctcaggaagc gcaggagaat
    20941 atcggtctgg aagttgcggg taaattgatc aagtattctg ataatggttc aacgctctct
    21001 gcggtgaact tcccggaagt ctcgctgcca ctgcacggtg gtcgtcgtct gatgcacatc
    21061 cacgaaaacc gtccgggtgt gctgactgcg ctcaacaaaa tttttgccga gcagggcgtc
    21121 aacatcgccg cgcaatatct gcaaacttcc gcccagatgg gttatgtagt tattgatatt
    21181 gaagccgacg aagacgttgc cgaaaaagcg ctgcaggcaa tgaaagctat tccgggtacc
    21241 attcgcgccc gtctgctgta ctaaatcccc ttatctgaaa atcaacgggc aggtcactga
    21301 cttgcccgtt tttttatccc ttctccacac cgttgccgca ttgcgcattc tactttgcga
    21361 gtctgctcca gaaaataaat caaagtcaga tcaatgcgtt gtgtttattt aatggggatt
    21421 tacgatggca gggcagcatg gggctgtaac ggtaaagccc tcaccgaagc gagggcttga
    21481 aggagaaggg ttatgatgcg acttgtcatc atactgattg tactgttact cataagtttc
    21541 ccagcttatt aacagtcagt ctcaggggag gagtcatcct cccttaccct tactcactaa
    21601 attaggttaa agaatcaacg atgtcaatca gggcgatgca ggtgtgtcgc cctcaccact
    21661 cccagacttt cgacggtgta accaccgcag gaagagggat atcccactct tcaacgggga
    21721 gtttttccac caactgacaa tcatgcgcat aacccaccgg ttgcatttta tagtgctgcc
    21781 agttttgtaa ggtccgatca taaaaaccac cgcccattcc caggcgctga ccgtactcat
    21841 caaaggcgac cagcggtgtg atcaacacgt ctaatcggga aaggggtagc acgtcacgca
    21901 catccagttt gggctcatgg atcttcaacc tgttcatcac cagttcgctt tgcggatggt
    21961 aattcaggaa cagcaaatta ccggcactga agggatgcaa aactggaaga tatacgcgct
    22021 taccggcgcg ccagagttgt tctatgagtg gcagggtgtc gagttcgcca tcaaaagaga
    22081 ggaatacagc gaccgtatgt gccatcacca ccgggggata agtcatcatc cgggtagcgg
    22141 cttgttgacc catttcctgc tgttgttccg gcgttaacgc acgacgacgt tgccgaatca
    22201 ttttgcggat ttcttgtcgg gataatgtca gtgggagttc tggtagttgc gtcatggctg
    22261 gtgccagata agaagggaat ctccgagatg ccgccgcagg ctgtaaccct tgaacccttg
    22321 gttcaaggtg aatgtgtcgt cgcagtttta aggcttctcg gacggaccga gcatgctcac
    22381 caaccgcgga gcgccacatt cttgtggtat gaaatatcgg ctcaggggac tggcccgctt
    22441 gcgaacatct cagagaaatt ttgtcttcac ggttactcta ccacagtaaa ccgaaaagtg
    22501 tcattcaaag ttttggttag ttttttcggt gatgcgacct tgttcaaata acgcttgttc
    22561 tatggtctgc tgcagcatcc gaatacgctg ttccatactt gccgcatagt cacgagtctt
    22621 cgctttttct tgcgctaact catagctgat attcaatgcg gcaatgaaga ccaactgttc
    22681 agtatttgtg actctagtgc gttctttcag atcttgcaac cgttggttca gatcgtccgc
    22741 tgcctgattc aacgcatccc tttggtcagg cgggcagttc acacgcagtg aacggccaaa
    22801 aatttggata tcgacgggtt gtgcagacat gccaccttcc tgctgattga ctgcgctgcc
    22861 ttcgtcttta gacccatggt ctgcgaaggg gcgacactat agctaccctg atgagaagag
    22921 acaagccctt ttctggtcca ccagggccca aagtggtagc atatcatgaa tattcctccc
    22981 tttgacgacg aatgcttatg tctatacaga acgaaatgcc tggttacaac gaaatgaacc
    23041 agtatctgaa ccaacaaggg acgggtctga cccctgctga gatgcatggt ttaatcagcg
    23101 ggatgatatg tggcggtaac gatgacagct catggctgcc gctacttcac gacctgacga
    23161 acgaaggcat ggctttcggt catgagctgg cacaggcact gcgtaaaatg cactctgcca
    23221 ccagcgatgc cctgcaggat gacggcttcc tttttcagct ttatctgcct gatggcgatg
    23281 atgtcagcgt tttcgatcgg gctgatgcgc tggcaggttg ggtcaatcac ttcctgcttg
    23341 gtcttggcgt tacgcaaccg aagctggaca aagtgaccgg agaaaccggt gaagccatcg
    23401 acgatctgcg taacatcgcg cagctgggct acgacgaaga cgaagatcag gaagagcttg
    23461 aaatgtcgct tgaagagatc atcgaatacg ttcgtgttgc cgcgctgtta tgccacgaca
    23521 cctttactca tccgcaaccg accgcgccag aagtacaaaa accgactcta cactaaaaac
    23581 aaaaaacgta aggagagtgt tatgagtgag atatcccggc aagagtttca gcgtcgccgt
    23641 caggccctgg tggagcaaat gcaacccggc agcgccgcgc tgatttttgc tgcaccagaa
    23701 gtaacacgta gcgccgacag cgaatacccc tatcgtcaga acagtgactt ctggtacttc
    23761 accggcttta acgaaccgga agcggtgctg gtgctgatta aaagcgacga cactcataac
    23821 cacagcgttt tgtttaaccg cgttcgcgac ctgacggcag agatctggtt tggtcgtcgc
    23881 ttaggccagg atgccgcgcc agagaaactg ggcgttgacc gcgcactggc attcagcgaa
    23941 atcaatcagc aactttatca actgcttaat ggtctggatg tggtttatca cgctcagggc
    24001 gaatatacct atgctgatga aatcgtgaac agcgcgctgg aaaagctgcg taaaggctcg
    24061 cgacagaatc tcactgcccc ggctacgatg atcgactggc gtcctgttgt tcatgaaatg
    24121 cgcctgttca aatcaccaga agagattgcc gtacttcgcc gcgcgggtga aatcaccgca
    24181 ctggcgcata cccgggcgat ggaaaaatgc cgtccgggga tgttcgagta ccatctggaa
    24241 ggcgaaattc accacgagtt taaccgccac ggtgcgcgtt acccttccta taacaccatt
    24301 gttggcagcg gtgaaaacgg ctgcattttg cactacaccg aaaacgagtg tgaactgcgt
    24361 gacggtgatc tggtgttgat tgacgcgggt tgtgaataca aaggttacgc tggcgacatt
    24421 acccgcacct tcccggtcaa cggcaaattc actcaggctc agcgtgaaat ctatgacatt
    24481 gtgctggagt ctcttgaaac cagcctgcgc ctgtatcgtc cgggaacttc catgcaggaa
    24541 gtcactggtg aagtggtgcg tatcatggtt agcggcctgg taaaactcgg catcctgaag
    24601 ggtgatgttg atgaactgat cgctcagaac gcccatcgtc ctttctttat gcatggcctt
    24661 agccactggt taggactgga tgtccatgac gttggcgttt atggtcagga tcgctcgcgc
    24721 attctggaac cggggatggt actgaccgta gagcctggtc tgtatatcgc gccggatgcg
    24781 gatgtgccag aacaatatcg cggtatcggc attcgtattg aagacgacat tgtgattacc
    24841 gaaaccggca acgaaaacct caccgccagc gtggtgaaaa agccggaaga aatcgaagcg
    24901 ttgatggctg ctgcgagaaa gcaatgagcg taatcatcgt cggtggcggc atggcgggcg
    24961 cgacgctggc gctggctatt tcccggttaa gtcacggggc gctgccggta catttgattg
    25021 aagcgactgc gccagagtca catgctcatc cgggctttga tggacgagcg atagcgctgg
    25081 cggcgggtac ctgtcagcaa ctggcgcgca tcggcgtctg gcaatctctg gcggattgcg
    25141 caaccgccat cactaccgtg catgtcagcg atcgtggtca tgccggattc gtcaccctcg
    25201 ccgcagaaga ttaccaactg gcggcgctgg ggcaggttgt cgaattgcat aatgtcgggc
    25261 aacggctgtt tgcattgctg cgcaaagcac ctggcgtaac gctgcattgt cctgatcgcg
    25321 tggctaacgt tgcccgtact cagagtcacg ttgaagtgac gctggagagt ggcgagacgc
    25381 tgacgggctg cgtgctggta gccgctgatg gcacccattc agcgttagcc accgtgtgcg
    25441 gcgttgactg gcagcaggag ccttacgaac aactggctgt gattgccaac gttgcgactt
    25501 ccgttgcgca tgaagggcgc gcttttgaac gctttacaca atatggcccg ctggcgatgt
    25561 tgccgatgtc tgacggacgc tgttcgctgg tctggtgtca tccactggaa cggcgcgaag
    25621 aggtactgtc ttggagcgac gagaagtttt gccgtgaact ccagtcggcc tttggttggc
    25681 gacttgggaa aattacccac gctggtaaac gcagtgctta tccgctggcg ttaacccgcg
    25741 ccgccagacc tattacccat cgtaccgtgc tggtgggcaa tgcggcgcaa actctgcacc
    25801 cgattgccgg gcaagggttc aacctcggta tgcgagatgt catgagcctt gcggaaaccc
    25861 tgactcaggc gcaggagcgc ggagaagaca tgggtgatta cggcgtattg tgccgttatc
    25921 agcagcgtcg acagcgcgat cgcgaagcaa ccatcggcgt cacggacagc cttgtacatc
    25981 tttttgccaa ccgttggggg ccgctggttg tcgggcgcaa catcgggctg atggcaatgg
    26041 aattattcac cccagcacgc gatgtgctgg cgcagcgcac cctcgggtgg gtggcgcgtt
    26101 gaaggcggtt ttaaggaata aacaatgcaa agtgttgatg tagccattgt tggcggcggc
    26161 atggtggggc tggcggttgc ctgcggcttg cagggtagcg gcttacgcgt tgccgtactg
    26221 gagcagcgcg taccggaacc tctggcggcg gatgcaccac cacaactgcg cgtttcggct
    26281 atcaatgccg ccagcgaaaa attactcacc cgtcttggtg tctggcagga cattctctct
    26341 cgtagggcca gttgttatca cggtatggaa gtgtgggaca aagacagttt tggtcacatt
    26401 tcgtttgacg atcaaagcat gggctatagc catcttgggc atatcgttga aaactcggtg
    26461 attcactacg cgctgtggaa caaagcgcag cagtcgtcag atatcactct gttggccccc
    26521 gcagaattac agcaggtcgc ctggggagaa aatgaaacct tcctgacgtt gaaagatggc
    26581 agtatgttaa cggcgcgtct ggtgattggc gcggacggcg ctaattcctg gttgcgcaac
    26641 aaagccgata ttccgctgac tttctgggat tatcagcatc acgcgctggt agcgaccatt
    26701 cgcacggaag aaccgcatga tgcggtggcg cggcaggttt ttcatggcga aggcattctg
    26761 gcctttttgc cgcttagcga tccgcatctt tgctcgattg tctggtcact gtcgccagag
    26821 gaagcgcagc ggatgcagca ggcaggcgaa gacgaattta atcgcgcgtt aaatatcgct
    26881 tttgataatc gcctgggctt atgcaaggtt gaaagtgagc gcctggtgtt cccgctaacg
    26941 gggcgttatg cgcgccagtt tgccgcacac cgtctggcgc tggtgggcga cgccgcgcat
    27001 actattcacc cgctggcggg gcagggggta aatctcggct ttatggatgc tgcagagctg
    27061 attgctgaac tgaaacggtt gcatcgtcag ggtaaagaca tcgggcagta catttacctg
    27121 cgtcgctatg agcgtagccg taagcacagt gcggcgctga tgctggctgg tatgcaggga
    27181 ttccgcgatc tgttttccgg taccaatccg gcgaaaaaat tgctgcgtga tattggtctg
    27241 aaactggctg atacgcttcc tggcgttaag ccacaactta ttcgtcaggc aatgggatta
    27301 aacgatttgc ctgaatggct gcgttaaata tttctcttct gctgtttatt tgatacccat
    27361 cacactttca tctcccggtt ttttcgccgg gagattttcc tcatttgaaa taaactaa
//
LOCUS       DAZKJR010000065        26940 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKJR010000065 DAZKJR010000000
VERSION     DAZKJR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.065"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..879
                     /locus_tag="ACHOWS_002703"
     CDS             <1..879
                     /locus_tag="ACHOWS_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0048252.1"
                     /translation="KFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQRLRAQF
                     NDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFIIYGPQLISC
                     TNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVISRSVICMPL
                     HTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDERFINRKVSF
                     RSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLYISYNKASL
                     NNLVFSRFIDRLNDSF"
     gene            1026..2246
                     /locus_tag="ACHOWS_002704"
     CDS             1026..2246
                     /locus_tag="ACHOWS_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFQ0048253.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDMPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2219..2848
                     /locus_tag="ACHOWS_002705"
     CDS             2219..2848
                     /locus_tag="ACHOWS_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFQ0048254.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLIPNDYNPNNVAPPEKKLLKKSIETDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2849..4009)
                     /gene="ybdL"
                     /locus_tag="ACHOWS_002706"
     CDS             complement(2849..4009)
                     /gene="ybdL"
                     /locus_tag="ACHOWS_002706"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFQ0048255.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VKFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4118..5206
                     /gene="hcxA"
                     /locus_tag="ACHOWS_002707"
     CDS             4118..5206
                     /gene="hcxA"
                     /locus_tag="ACHOWS_002707"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFQ0048256.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFENVESFKA"
     gene            complement(5216..5413)
                     /locus_tag="ACHOWS_002708"
     CDS             complement(5216..5413)
                     /locus_tag="ACHOWS_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFQ0048257.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5526..7631)
                     /gene="cstA"
                     /locus_tag="ACHOWS_002709"
     CDS             complement(5526..7631)
                     /gene="cstA"
                     /locus_tag="ACHOWS_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFQ0048258.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALVALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7812..8225)
                     /gene="entH"
                     /locus_tag="ACHOWS_002710"
     CDS             complement(7812..8225)
                     /gene="entH"
                     /locus_tag="ACHOWS_002710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFQ0048259.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8228..8974)
                     /gene="entA"
                     /locus_tag="ACHOWS_002711"
     CDS             complement(8228..8974)
                     /gene="entA"
                     /locus_tag="ACHOWS_002711"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFQ0048260.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8974..9831)
                     /gene="entB"
                     /locus_tag="ACHOWS_002712"
     CDS             complement(8974..9831)
                     /gene="entB"
                     /locus_tag="ACHOWS_002712"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFQ0048261.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9845..11455)
                     /gene="entE"
                     /locus_tag="ACHOWS_002713"
     CDS             complement(9845..11455)
                     /gene="entE"
                     /locus_tag="ACHOWS_002713"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFQ0048262.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11465..12640)
                     /gene="entC"
                     /locus_tag="ACHOWS_002714"
     CDS             complement(11465..12640)
                     /gene="entC"
                     /locus_tag="ACHOWS_002714"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFQ0048263.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFAGAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12829..13785
                     /gene="fepB"
                     /locus_tag="ACHOWS_002715"
     CDS             12829..13785
                     /gene="fepB"
                     /locus_tag="ACHOWS_002715"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0048264.1"
                     /translation="MRLVPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAETVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLASGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13789..15039)
                     /gene="entS"
                     /locus_tag="ACHOWS_002716"
     CDS             complement(13789..15039)
                     /gene="entS"
                     /locus_tag="ACHOWS_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFQ0048265.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15150..16154
                     /gene="fepD"
                     /locus_tag="ACHOWS_002717"
     CDS             15150..16154
                     /gene="fepD"
                     /locus_tag="ACHOWS_002717"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFQ0048266.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLYTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16151..17143
                     /gene="fepG"
                     /locus_tag="ACHOWS_002718"
     CDS             16151..17143
                     /gene="fepG"
                     /locus_tag="ACHOWS_002718"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFQ0048267.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAITLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAASLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQLLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17140..17955
                     /gene="fepC"
                     /locus_tag="ACHOWS_002719"
     CDS             17140..17955
                     /gene="fepC"
                     /locus_tag="ACHOWS_002719"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFQ0048268.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(17952..19085)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOWS_002720"
     CDS             complement(17952..19085)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOWS_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFQ0048269.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEVDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGIVACGGVLLRHAMASRKQDAIMADHLV"
     gene            complement(19301..23182)
                     /gene="entF"
                     /locus_tag="ACHOWS_002721"
     CDS             complement(19301..23182)
                     /gene="entF"
                     /locus_tag="ACHOWS_002721"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFQ0048270.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPETIDLRTNIDPHGTAQSLMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFVRQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DSQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERALQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(23179..23397)
                     /gene="ybdZ"
                     /locus_tag="ACHOWS_002722"
     CDS             complement(23179..23397)
                     /gene="ybdZ"
                     /locus_tag="ACHOWS_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFQ0048271.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQVSCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(23400..24602)
                     /gene="fes"
                     /locus_tag="ACHOWS_002723"
     CDS             complement(23400..24602)
                     /gene="fes"
                     /locus_tag="ACHOWS_002723"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFQ0048272.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFRWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24845..>26940
                     /gene="fepA"
                     /locus_tag="ACHOWS_002724"
     CDS             24845..>26940
                     /gene="fepA"
                     /locus_tag="ACHOWS_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFQ0048273.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVD"
BASE COUNT         6468 a         7510 c         6930 g         6032 t
ORIGIN      
        1 aaatttgacc tcaatttact ggtcatattt gaatgtattt atcaacatct cagcattagt
       61 aaagcggcag aatcattata tataacgccc tctgctgtta gtcagtcgtt acaacgttta
      121 cgtgcgcaat ttaatgaccc gttattcatt cgctcaggga aaggtatcgc gccgactaca
      181 acagggttaa acctgcatca ccatcttgag aaaaatctca gaggccttga acaaacgatc
      241 aatatcgtta ataagtctga actaaagaaa aattttatta tctacggtcc tcagcttatt
      301 tcttgtacta ataacaacat gctaatccgc tgtcttagac aagatacttc aatcgatata
      361 gaatgccatg acatcctgct ttcagctgaa aatgccgaag aactgttggt tcaacgcaaa
      421 actgacctgg caattacact gcaaccagta attagtcgct ctgtcatctg catgccatta
      481 cacactattc gcaattccct tatatgtagc aacaaacatc ctcgtattac tgataccagt
      541 aactatgaac aaattatcgc cgaagaattc accctgttga tatctaaatc agcagggatt
      601 gacgaaattc aaatggacat agatgaacgt tttataaacc gtaaagtttc atttcgcagt
      661 tcttctttgt tgactataat taatagcatt tctgttactg acttattagg cattgtgccc
      721 accgaactgt acgatttaca tcgtgatttt ttgaagttaa aagaaataaa actagaacaa
      781 cctcttcctg cggttaaact ttatatttcc tataacaagg cttcgttgaa caaccttgtc
      841 ttctcccgat tcatagatcg tttaaatgat agcttctgat tcattaccac tgacaaataa
      901 tatattacgc atcaattaat tcattgtgtg aatataaaat aatgtgctct atcttgcttc
      961 gtttatccta taattagcgg attcatctca cgagtcaccc cgtaacgttc agagagcgtt
     1021 tttagatgtc tatttataaa atcccgcttc cactgaatat tctggaagct gcaaaagaac
     1081 gtattacctg gacacttaat accctacccc gtatctgtgt ttctttttcg ggtggtaaag
     1141 attccggatt aatgctgcat ctgacagccg aaattgcccg acaaatgggc aaaaaaatct
     1201 gcgttttgtt tatcgactgg gaagcgcagt tctcatgcac tattaactat gttcagtcat
     1261 tacgtgagtt ttacgccgat gtcatcgaag agttttactg ggttgcgctc ccgcttacga
     1321 cgcaaaattc cctttctcaa taccaacccg aatggcagtg ctgggaacct gatgtcgaat
     1381 gggtgcgtca gcccccgcaa gatgcgataa ccgatcctga ctttttctcc ttttaccagc
     1441 ctggcatgac cttcgaacaa tttgtgcgtg agtttgctga atggttttca caaaaacgtc
     1501 cggcggcgat gatgatcggc atccgtgcag atgagtccta caaccgtttt gtcgccatcg
     1561 ccagtttaaa taaacaacgt tttgccgatg atatgccctg gaccaccgcc gcaccaggcg
     1621 gtcatagctg gtacatttac cccatctacg actggaaagt ggctgatatc tggacctggt
     1681 atgctaacca tcaacagctc tgtaacccac tgtataatat aatgtatcag gcaggcgttc
     1741 ctctgcgtca tatgcgaatt tgcgaacctt ttggcccgga gcaacggcaa ggattatggc
     1801 tctatcacgt tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa
     1861 aaagtggcgg catttacgcc ggacatgata atcattttta tggtcatcgg aaaatcctca
     1921 agccagaaca tttagactgg caagaatatg cattattgct gctcaatagc atgccggaaa
     1981 aaacagctga gcattaccgc aataaaatcg ccatttattt gcactggtat cagaaaaaag
     2041 gcatcgaagt tccacaaacc cagcaagggg acattggcgc gaaagatatc ccctcctggc
     2101 ggcgaatatg caaagtttta ctcaataacg attactggtg ccgggcatta tcatttagtc
     2161 ctacgaaagc gaagaactat cagcgttata acgaacggat aaaaggaaaa cgtcaggaat
     2221 gggggatact atgcaacaac gattaacgca ggatttaacc caatttctcg ccagcttgcc
     2281 ggaagatgat cgcatcaaag cgattaatga aatccgcatg gcgatccatc aggttagccc
     2341 ctttcgtgaa gagccagtgg attgcgttct gtgggttaaa aacagccaac ttatacctaa
     2401 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgaaaa aatccattga
     2461 aacagatggt tttacccaac ccattgtggt cacgcatacg gataaaaatg ccctggaaat
     2521 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcgttaa aattacgctt
     2581 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
     2641 tattcgtcat aaccgcgctc gtggtcgcca ccaaatcact gccatgtccg aaattgttcg
     2701 tgaactcagc cagttgggat gggacgataa taaaattggc aaagagctgg gaatggacag
     2761 cgacgaagtg ctgcgcctga aacaaattaa cggcctgcaa gagttgtttg ccgaccgtca
     2821 atattcccgt gcctggactg tcaaataact acagctggcg caggcgttca gctgctgcca
     2881 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatggggg aagggatcgg
     2941 cgcaaaatac cgacagcggg atcgccgcta caccgtgctc ccgcgtcagc cactggcaaa
     3001 acttaacgtc atctagcgaa gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
     3061 cgcacggtaa aatctccagc cgactctcac ttaaggcatt caccagaata tcgcgtttct
     3121 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcggcaa
     3181 gcgccaactg tgccggggta ttcaccgaaa aggtcagata ctgatgcact ttgcggatct
     3241 cggcgctgat gggcgcaggc gcaacgcaat aacccacttt ccagccggtc atatgatagg
     3301 tcttgccaaa cgatgacacc gccaccgctc gctcacgcaa ttgcggatgc gccagcacgc
     3361 tggcatggcc ctgttgtgaa aaattgatgt gctcgtagac ttcatcgcta atgacaaaaa
     3421 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     3481 cactggggtt atgcggggtg ttgaggatca ccagtcgggt gcgctcgctt aacaatgcgg
     3541 caaattcctg ccagtccacg cgaaaatgcg gtggttgcag tgcaatacgt ttcactattc
     3601 cccccgaaag cgcgatggcg ggcgcgtaac tgtcatagct gggatcaaag cagatcactt
     3661 catcaccatt gcgcaccaat gcggtaatcg ctgcatataa cgcttccgtc gcccctgccg
     3721 ttacggtgat atcgctatcg gcatccggtt gatagccata cagacgttcc gttttctgag
     3781 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     3841 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     3901 gtgaaaggtt aatcgcctgg tgttgctgcg ccagcgcgct catccgggtg aaaatagtgg
     3961 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     4021 ctgacgaagt tgcgtgttga aggcactata acacgatgct agtatttggc aatcaagacg
     4081 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     4141 ggtcgtcggc ccggctaact acttttcaca tcccggaagt ttcaatcacc tgcacgattt
     4201 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcgaacgcg ccattgctgc
     4261 ggcacaaacc aaactgccgc ctgcgtttga actgccaggg gtaaagcata ttttgtttcg
     4321 tggtcattgc agcgaaagcg atgtgcaaca actggcggcg gagtccggtg acgatcgcag
     4381 cgtggtgatt ggcgtcggcg gcggcgcact actcgacacc gcgaaagccc tcgcccgccg
     4441 tctcggtctg ccgtttgttg ccgttccgac gatcgccgcc acctgcgccg cctggacacc
     4501 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     4561 caattttatg gtgctggtgg agccagagat tatcctcaat gcgccgcaag aatatctgct
     4621 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     4681 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     4741 cttgttaaac agtagcgaac aggcgctgag cgatcagcaa aaacaacagt taacgcaatc
     4801 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     4861 tcgttttacg cgtgtggcgg cggcccatgc cgtgcataac ggtctgaccg ttttgccgca
     4921 aaccgagaag tttctccacg gcaccaaagt cgcctacgga attctggtgc aaagcgcctt
     4981 gctgggtcag gatgatgtgc tggcgcaatt aacagaggcg tatcagcgtt ttcatctgcc
     5041 gactacgctg gcggagctgg aagtggatat caataatcag gtggagatcg acaaaatgat
     5101 tgcccacacc ctacgtccgg tggaatccat tcattacctg ccagtcacgc tgacaccaga
     5161 tacgttgcgt gcagcgttcg aaaacgtgga atcgtttaaa gcctgacgta caaatttagc
     5221 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcccgg aaaaactcct
     5281 cgtaggtcat cggtgtttga tcgggatggt taacccgcat atgttcgaca tagttgtcgt
     5341 aatcaggcat accaatcatc agtttcgccg cctgacctaa atattttccg gcttttgcca
     5401 gtgaatcaaa catctaaacc tccggtaata caaatgccct cctgaattaa gagggcttaa
     5461 ttgctatcaa cacgctcggg atgagagtaa ggggaacgct cagcacattc cccctcactc
     5521 tgactttagt gcgcgccttt cgcctgcgcc acgatctcct cgacattttc cggcatcggc
     5581 tcatatggcg tttctttcgc cgttggcttc ggctctttca atgccaccag tgccgtctta
     5641 atcgagaaca gcgccagaac caccacaacc accataaaga agatggttaa tccggcatcc
     5701 agacggttgt tgaacaccag ttgcgccagt tgtgactcag tatactgcga cggaatattg
     5761 ccgctgtcga tcattgcctg gaacttgtta gcaatcgcca ggaagccgac tttcgcatcc
     5821 gggctaaacg ctttctgcca gcctgcggtc agggtacaaa tcagcagcca ggccgttggt
     5881 accagcgcca cccaggcgta acgttgacgt ttcattttga acagcaccac agcacagagc
     5941 atcagcgcca tccctgccag catctggttg gcaataccaa acagcggcca caaggtgtta
     6001 ataccgccca gcggatcgac cacgccctga tgcaggaagt agccccacgc cagcacgcac
     6061 agcgccgttg ccagcaggtt agcaggcagt gaatcggtcc gtttcaggcc cggagacacc
     6121 acgcccagca gatcctgcaa cataaagcgc gcagcacgcg tacccgcatc caccgccgtc
     6181 agaataaaca gtgcttcaaa cagaatggcg aagtgatacc agaacgccac atccatcatg
     6241 ccgcccagcg caccgtgcag aatgtaggcc atccccaccg ccagggtcgg cgcaccgccc
     6301 gcacgagaaa tgatcgactg ttcacccact tcgctggcaa tctggtttag cgtatccggg
     6361 gtaatcgcaa agccccagct actcaccacc tgcgcggcag aagcaaccac atccgccgtc
     6421 cctgccggag ccagcaccgc catcgggctg ttcatggcga agtacacacc cggatcgatg
     6481 atacaggcgg aaaccagcgc cataatcgcc acgaaggatt ccatcaacat cccaccgtag
     6541 ccgataaagc acgcctgccc ttcgttcgcc agcatcttcg gtgtagtgcc ggaagagatc
     6601 agtgcatgga agccagacac cgcgccacag gcgatggtga taaacaggaa cgggaacagg
     6661 ttaccggtcc ataccgggcc agtgccatcg acaaatttag ttagcgcagg catggtcagc
     6721 gtcgggcgca taatcaaaat gcccaccgcc agaccgacga tagtcccgat tttcaggaag
     6781 gttgagaggt agtcacgcgg agccagcagc aaccacaccg gcagcaccgc cgccacaaaa
     6841 ccgtaaccca ccagcatcca ggtcagctgc acgccggtaa agtcaaagta cggtgcccac
     6901 gtcgggcttt ctgccaccca gccgccagag ataatggcga aaataaggaa caccagacca
     6961 atcaccgaca cttcgccaat acgccccgga cgcaaatagc gcaggtagat ccccataaac
     7021 agcgccagcg gaatggtgaa cgcaacggta tacgttcccc acgggctatg agtcagggct
     7081 ttcaccacga tcatcgccag cacggcaagg ataatgacca tgatcataaa gcaggccacc
     7141 agcgcaatca ccccggcggt tggccccatc tcttctttga ccaactcacc cagcgagcga
     7201 ccatcgcggc gcgtagaaac aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc
     7261 accccagcga gcagccagat catccctggc aggtaaccca tttgcgccgc cagtaccggc
     7321 ccaaccaacg gacctgctcc ggcaatggcc gcaaaatggt gaccgaacag cacttttttg
     7381 tccgtcggca catagtccag cccgtcgtta tggcgcaccg ctggcgtcat acgcgtcggg
     7441 tcaaccgcca gcacattttt ggcgatatac agcccataaa aacggtaagc gatcagatag
     7501 atacagaccg acgccaccac aatccacagc gcgttgatct gttccccacg atttaaagca
     7561 atgtatccca gagcaaatgc tcccattaca gagagcactg tccagacgag gtatttccct
     7621 gatttgttca tagttgttat ccgtgtgcgt gtccatagag atgttacatt ttgtttctat
     7681 aacatctttg tacaatttaa caacacggca accatgtaaa tcggagttga aaccaatatt
     7741 taacttaaca atgttaactc gatcactccg ttaaccgagt tttcctgcaa tctcatttaa
     7801 ttctgtcggg ctcatcccaa aactgccgta cccagccgac aagtgcagca acgccgcccc
     7861 tgttcatcga aaacaacaat ttcccagctc tgattttgcc gaccaagatg cagcggctgg
     7921 cagacgccgc gtacctttcc ctcagacacc gggcgatgat gtgtggcgtt aagttccgtg
     7981 cccaccacac actgtccgtc gcgagtcatc ataaatccgg ccatcgatcc cagcgtttcc
     8041 gccagcgccg ccgacgcgcc gccatgtagt aaaccgaacg gctgatgagt acgggtatca
     8101 accggcattt cggcttccag cacatcatcg cccaggcggg tatacacaat tcccagatgc
     8161 gccaccattg tgttatcgct ggtggcgttc agctcgtcga gcgttaaatg gcgtttccag
     8221 atcatgctta tgcccccagc gtagagccgc catctaccac aatatcctgt agggtgatat
     8281 ggctggcgag gtcagaggcg aggaacaaaa tcgtgttggc gatctcttgt ggacgggcga
     8341 ttttccccag cggaatgcca agtttaaact gctcgccaaa gccgcgaata cgctgctctt
     8401 cggcgtcatc gctcacccac agcgtgcgtt gcatatcggt gtcggtggag cctggcgaaa
     8461 ccacattaca gcgcacgccg ctacccgcca gttccagccc aacgctcaac gccaggcttt
     8521 tcagcgccgc tttcgacgcg ccataagcac tcataccaat gcgcggcgtg tgtgcggcgt
     8581 cggacgcaac agtaacaatc gccccgcccc gctgacggcg aaactggttc atggtctgct
     8641 ggaacaggtt aaacgcaccg ccgacattaa ccgcaaaagt ctgctgccag tcctccttac
     8701 tgagctgatc ggtcgcgccc atgcgcaaaa ttcccgccgc attgaccagc acgtccagtc
     8761 gctccgtttc agctaacagt cgctgacata cctgcgccac ctgcgcagcg tcggcaacat
     8821 ccatcacttc ggtcgcaaag ggatattgct cctgagtgaa cgcttgatca aaacctgtaa
     8881 ctttcgctcc cgcctcaaca aacgccagcg ccgtggcgta gccgatacct ttccccgcgc
     8941 cagttaccca gacattttta ccgctgaaat ccattatttc acctcgcggg agagtagctt
     9001 ccaccaggcg tcgatggtcg ggtttttcgc cagcatgaca aagtcgatat cgccatgcac
     9061 tttgcgccag cgcgccgcca gcgccatcat ccgcaccgaa tccagaccgt agtcgatcag
     9121 gttgtcgtca tcgaacggtt catcggactc gtccagcaac ggcaggatca cctcacgcag
     9181 cgccgctttg ctggcgggga taggtgctgg cagtaattct tcggtcatca ccactcggcc
     9241 agaacgtccg gccacatatt tcagcgacat caaatgctcg tcacggctga aatcggccag
     9301 cgcatctgcc accataaacg gtttaatatc gcgcataaat gcgtcggttg cggtggtcat
     9361 acagccaatg tgggcatata ccccggtaat aatcagctgg ttacgtccac tctctttcag
     9421 catttgctcc agcggggaac gatgaaacgc gctgtagcgc cacttcacca gcaccgtgtc
     9481 gtcggcatct ggcgtcaggc tatccaccac cttttgctgt tccggcgaac gggtcaggcc
     9541 aggcccccac atatcattca acagcgcccg atcttcatcg ctctgctctt tcggctgggc
     9601 ggtgtaataa accgggatat tgtgctgttt gcagtagtcg cgtagtgccg caatattcgc
     9661 gatcacctgc tccatcatcg ggcagttctc gccccagaag ctgacaaaat agtcctgcat
     9721 atcatggatt aacaacgcgg cacgttgcgg ttcaaaggcc cagtcgactt tattctgcgg
     9781 aatatcgtga gactccggca gtgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc
     9841 tccttcaggc tgatgcgcgt gacgccagcc actgacgtaa ttgtttttta tcgactttcc
     9901 cgaccgccgt cagcggaagt gaatctacac actccacgcg atccggtaat ttaaattcgg
     9961 caataccctg ttcacgcagg aaacgacgca cctgcaccgc gcgcagcggc tcttttacca
    10021 ccagataagc acagcttttt tcgcccatca gctcatcttc catgctcacc agtgcggcgt
    10081 agatcaccgc cggatggcgc agcagcaggt tttcgatctc ttcggcagcg atcttctcgc
    10141 cgccacggtt gatctgatct ttctcgcgcc cctgcacagt gatgtaaccc tctggatcaa
    10201 tagagatcag atcgccggaa cagtaaaaac cgttggcatc aaaggcgctg gcattgtgct
    10261 gcgggctttt gtaatagcca cggaaggtat acggaccgcg ggtcatcagg cgtccaactt
    10321 ccccttgcgg cagtggattt ccttcggcat cggcaaccca cacttcatcg tccggacaca
    10381 ttgggtaacc ctgggtatgg ataattttct ccgcgctatc atcaagtcgg gtgtagttca
    10441 ctagtccttc cgccatgccg aatacctgct gtaactggca gccaatctct gcgggaatac
    10501 gcgccgcaag cgtggcagaa agacgtgcac caccgacctg taacagtttc agcgaggcaa
    10561 gctgcgcccg gctttcgcct tcagccagtg cctgcaacca caggctgact gcgggcggca
    10621 ccagcgcggt aacgttgacc tgatgttttt caatcaatgg gaagcaaagc gtggcgctgg
    10681 ggtcggcagc cagaacgaca gtgccgccag cgagaaatac ccccaacgat cccggcgaac
    10741 tcatggcgta gttatgagcc gccgggatcg cgcacaggta gcgcgtctgt tgtgtgaact
    10801 gacaaatctc gacgctacga cgcacgctgt agtagtagtc gttatgagtg cgcggtatca
    10861 gtttcggagt gccggtggtg ccgccggaaa gctggaaata ggccacttca tcagcaggag
    10921 atggcgtggc agtaaaatcc tcagccggat ggttaatcgc atcctgcaag ttatgctcac
    10981 cgctgtcgtt gagcagctgc accacgcgaa tggaagaatg ctctgcaaca aatgtgttga
    11041 ggaaatcatc cccgctaaat agcgcatgtt gacgatcggc aatcagtaac gccggttcaa
    11101 tctggctggc ataggcgttc agttcactac gctgatggct gaacagcgcc agcaccggcg
    11161 caacgcccag cttcagcagc gcgaaaaagg taatatacaa ttcagcgacg ttacccagtt
    11221 gtaccagcgc ggtttcacca ggtttaatgc cctgacggcg caaactacag gcgaggttat
    11281 ccgccgcctg attcagctcc cggtaactca actgccgctc gccgtcgata accgcgatgc
    11341 tgtcactcgc agcgtggcga gtcagaatgt cggtcagtgg caaatcctgc cagtagcctt
    11401 tttcccgata gcgacgggca aactcttccg gccagcgggt gaatggaatg ctcatcctcg
    11461 ctccttaatg caatccaaaa acgttcaaca tggtagaaag tttgacgcct gtttcgcgcc
    11521 actcacccaa cggtgaggac gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat
    11581 tttcccgcag cttcgcgcag cggatggtca ccacccattc accgttacct tcgctgtcac
    11641 accaacccac aatgccgcca aacagttcgc ggtcgaacgg ctccagttca gcaataacct
    11701 gagtcgcggc ctgatgcggg aaaccgctca gcgcgggggt cggatgcagc agacaggcca
    11761 gagtcagtgc gttttcttgc gaattcgctt taccttcaaa gggagttgcg agatgccaca
    11821 gcgtcggcgt ggtaatcaat tgtggagagg agggaacgtg taactcacta ctgcgtttgc
    11881 gcagtacctc tttcatcgcc tgagtcacca gttcatgttc atggcgatct ttttctgacg
    11941 ccagcagacg attacccgct tcgcgatcga gcacttcatc cggctgacga cgcgcggaac
    12001 cggctaacgg aatggagcta aaacgctcgc cgtctttacg tagcagcagt tccgggctgg
    12061 cccccagcag gacgccgcca tcagccagcg ggacatggaa gttgtaacta accgggtttt
    12121 gcgcaatcaa ccgttccagc aatacgccac tatcaatggc ggcgtcagtg gtgatatcaa
    12181 tcaaccgtga caacaccact ttgtcgacct gcggcgtggc ggtaagtgcg gcggcgcggg
    12241 caaccatctg ttcaaacgtg gtttgttccg gaattgcctg gcgttccacc acattcagcg
    12301 actggctgcg ggtgaaacgg cgtgcggaag cttgtttttc ctgacgggag aacgactgcc
    12361 aggattcggg aatatacagc gatgaaggct gacgtggatc gaagggaatc gccccgacca
    12421 tcaccggatt tttgatgccc tgcgctttgg caccggcaaa cagcgcggcg agtttttgct
    12481 ggaagggact gtcgggcgaa tccccgttca cagccggttc atcgaagcgg gcgaaacatc
    12541 ctgacgtcgt aaaactgcgg tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg
    12601 ccatggtctg ctgtacttcc tcagccagtg acgtatccat atcatcctcc acaaaatgat
    12661 aaaggcttta ataatgatta tcatttatat tttcggtcgc taacctaaaa gcaaacgcgc
    12721 actatgtcaa ctcttgaggt aacgcaatct gcaactcggg gttgcatctg gttgtggtta
    12781 caatgaaaat gagaagcatt aacttcatta ataacaggaa gctgatttgt gagactcgtc
    12841 ccgctctacc gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt
    12901 caggccgccg actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc
    12961 cagccgcagc gtattgtttc caccagcgtc accctgactg gctcactgct ggcgattgat
    13021 gctccggtga tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcgga tgaccagggc
    13081 tttttacgcc agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc
    13141 gagccgagtg cagaaaccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc
    13201 ggcggtgatt cagcgctggc gctgtacgat cagctttcca ccatcgcccc aacattaatc
    13261 atcaattacg acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg
    13321 catgagaaac aagcggcaga gcggattgca cagtttgata agcaactggc ggcggcgaaa
    13381 gagcaaatca aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac
    13441 agtgccaatc tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt
    13501 acgttggcga aattgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac
    13561 atcattcagc ttggtgggga aaatctggct tcagggttaa atggcgagtc actattcctg
    13621 ttcgccggtg atcagaaaga tgccgatgcg atttatgcta atccgctgct cgcgcacctg
    13681 cctgcagtac aaaacaagca ggtttatgcg ctgggaaccg agacgttccg tctggattac
    13741 tacagcgcca tgcaagtgct ggaacggctt aaggcgctgt tttaagcatt aactgtcgga
    13801 cgctgtcacc tgcggcggcg tctggcgaaa acgccgcaac tccaccagca ccagcaataa
    13861 caacacgccg ataatcaaca aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat
    13921 catcgcgccc aaaccgccca gcagcgccgc gcctatggca tcgcccgtca cgttttgcgc
    13981 cgtccacaaa ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt
    14041 gtattgcagc aacgaactaa ccgcgctcag ccagccgaac agcgccagac aaaccacgcc
    14101 taaaatccac atcggcatca ggccaaacag accaatggcg aggaacgatc ccagcgtgga
    14161 aagcagcatc aataaccctg gtcgcgcgct atgtgccagc ttcccgctgg ttaacgcgcc
    14221 aatagccgca ccgagcggga tcgccgcata gagaaaacca atctgtgccg ccgacatctg
    14281 ccagttgtca gccagcgccg gatacaggac ccgcaccgcg ctcgccatcg tcaataaacc
    14341 acccaacagc gcaatccctc ctaccagcgg gctggcgagc agaaaacgaa atcctgccag
    14401 taatgatttc aacggatgct cacgcggctg cggtggtggt ggtaacgccg gaaggcttaa
    14461 caacggtagc aaggtaataa acgtgcccgc cgccgccagc ccgtagttcc aggccacacc
    14521 gccagtcgcc agcagtaaac cgccaatcat gggcgaaatc accgatccca gacgcacggt
    14581 caacatggtg atcgccccgg cctgcattaa gttttcacgc cctaccagtg ctggtgtcgc
    14641 cgccagtagc gccgtaacgc caagtgatgc gaaaaaacca tcccataaac caagtaaata
    14701 gattgccagc aatgacggct ccggcagcag tgcattaagg cacagtccaa tgaagccaat
    14761 gccacaggtg ccgcgcgcca gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac
    14821 accgccgacc atcaggccaa caaacatcgc gccgccggtc agcgtcaccg aaagccccac
    14881 ctgccaggta gaatgcgtca ttatctggat ctgcaccggt accgcgacgc cgagcaaacc
    14941 cagagacaca attgagataa aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt
    15001 caacaggctg aggttaagca gccaggattg tttattcatt acaatgcctt gccatcaaat
    15061 attttaaata cctattctta ggctgcacat gctaacatat ccaaataaga tcgataacga
    15121 taattaattt cattatcatg gaagttcgta tgtctggttc tgttgccgtg acacgcgcca
    15181 ttgccgtgcc cggattgctg ttattactga ttatcgcgac ggcattaagc ctgctcattg
    15241 gggcaaaatc actccccgct tccgtagtgc tggaggccct ctccggcacc tgccagagcg
    15301 ccgactgcac catcgtgctc gacgcgcggc tgccgcgcac ccttgccggt ttactggcag
    15361 gcggcgcgct tgggcttgcc ggggcgttaa tgcaaaccct cacccgaaac ccacttgccg
    15421 accccggctt gcttggcgta aacgccggag ccagctttgc cattgtgctg ggtgcggcgc
    15481 tgtttggtta ctcatccgcg caggaacaac tggcgatggc cttcgccggg gcgctggtgg
    15541 cctcgttgat tgttgccttt accggcagcc agggcggcgg gcagttaagt ccggtgcgtt
    15601 taaccctggc gggcgtggcg ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc
    15661 tgcttaatcc tgacgtctac gatcaactgc gtttctggca agccggttcg ctggatattc
    15721 gcaatctata taccttaaaa gttgtgctga tcccggtgct gatcgccgga gcaaccgcgc
    15781 tattactgag ccgcgcgctg aacagtttaa gcctcggcag tgacaccgcg acagcgctgg
    15841 gcagccgcgt ggcgcgcaca cagttgattg gtctgctggc gattaccgtg ctttgtggta
    15901 gtgcgacggc ggtagttggc ccgattgcct ttattggcct gatgatgccg catatggcgc
    15961 gttggttggt tggagcagat catcgctggt cgctgcccgt cacgctactt gccacccctg
    16021 ccctgctgct gtttgccgat atcatcgggc gggtgattgt gcccggcgaa ctgcgagttt
    16081 ccgtggtcag tgcgtttatt ggtgcaccgg tgctgatctt cctcgtgcga cgtaaaacgc
    16141 gaggtggcgc atgatttacg tctctcgccg attaattatc acctgtttgc tgctggtttc
    16201 cgcctgtgtg gttgcaggta tctggggatt acgcagcggt gccatcacgc tggaaacctc
    16261 gcaggtattc gccgcgctga tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga
    16321 atggcgttta ccacgcgtgc tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg
    16381 cgcgattttt cagtcgctga tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa
    16441 caccggggca tggagcggcg tactggtggc gatggtgctg tttggtcagg atctgacggc
    16501 tatcgcgctg gcagcaatgg cggggggtat tgtcacctcg ctgttggtct ggctgctcgc
    16561 ctggcgtaac ggtatcgaca cctttcggtt gattattatc ggtatcggtg ttcgcgccat
    16621 gctggtggcc tttaatacct ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc
    16681 gggtttgtgg aatgccggat cgctcaacgg cctgacgtgg gcaaaaacct caccttccgc
    16741 gccaatcatt atattgatgc tcattgccgc ctccttgctg gtgcggcgaa tgcgcttgct
    16801 ggaaatgggc gatgataccg catgtgcgct gggcgtcagc gtcgaacgtt cgcggctgtt
    16861 aatgatgctg gtcgccgtag tgcttaccgc tgcggcaaca gcgcttgccg ggccgatttc
    16921 ctttattgct ttagtcgcgc cgcacattgc ccgccgcatt agtggcaccg ctcgctgggg
    16981 gctaacccag gcggcgctgt gcggggcgtt gttactgctg gtggccgatc tctgcgccca
    17041 actactgttt atgccgtatc aacttccggt tggcgtcgtt accgtcagcc tcggtggtat
    17101 ttaccttatc gtcttgttaa ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt
    17161 ttgcgcggcg aacagttaac cctcggatat ggcaaatata ccgttgcgga aaatctgact
    17221 gtagaaatac ctgatggtca cttcacggca attatcgggc caaatggctg cggtaaatcc
    17281 acgttgctgc gtaccttaag ccgcctgatg acgcctgctc acgggcatgt ctggctggat
    17341 ggcgagcaca ttcaacatta cgccagtaaa gaggttgcac gccggattgg cctgttggcg
    17401 caaaacgcca ccacgccggg agatatcacc gtgcaggagc tggtggcgcg tgggcgttac
    17461 ccgcatcaac cgctgtttac ccgctggcgc aaagaagatg aagacgcggt aacgaaagcg
    17521 atgcaggcca cgggaatcac tcatctggca gatcaaagcg tggataccct ttctggcgga
    17581 caacgccagc gagcgtggat agcaatggtg ctggcccagg aaacggcaat tatgctgctc
    17641 gatgaaccca cgacctggct ggatatcagt catcagattg atttgctgga actgttaagc
    17701 gaactgaacc gcgagaaagg ctataccctg gcggcggtgc tgcacgatct taatcaggcc
    17761 tgtcgttacg ccagccattt gattgcattg cgggaaggga aaattgttgc tcagggagcg
    17821 ccgaaggaga ttgtcactgc tgaactgatc gagcgcattt atggtctgcg ctgcatgatt
    17881 attgacgatc cggttgccgg aacgccgctc gtggtgccgc tcggacgaac gacaccttca
    17941 accgcgaaga tttaaactaa gtggtctgcc attatggcat cctgttttct ggatgccatc
    18001 gcatggcgca atagcacgcc accacaagcc actataccgc cgatcaacgc agaaaggatc
    18061 acaataatcg ccttacccgg accgtctttt tttacgggta atgacggacg caactgatat
    18121 ttaaacggtg taaaattcac atcgttgaca tttgtttttg ttaattgctc gactagatac
    18181 tgacgattac gtaattcacc gttcaattcc gcaacgtcag tgactgcttt ttcaatttcc
    18241 agtttgcgtt caataccgtc tgcgccgaga gaaatagaga aatcggggtc atctttaacg
    18301 gcctgaccat tactgtatac gggcttttta attcctgccg cgttggcaat gtcgagtgaa
    18361 taattgaggc gctgaatgtt tgcatcaagt tgatttttcg ttttaatgcg atcctgagcc
    18421 agtttttctt tttcaaactg ggttttgatc tccagtttat tacggacgtt ttctatcgac
    18481 tctttcacca ccaacgcaga gatataatcg atatacccgg ccaacacttt ctgtgcctct
    18541 tcactggtgg gtgcggtgaa acttagcgtc caggaagtat acaatgcaga ctcatctttt
    18601 ttcttactgg cattgtcatc aaccgctttc attttttcgc tcaatgcgac aattgcgcga
    18661 tgcaaatcca gttcgtcgat tttcgcctct tttaattggt ccatcacata aggtgttgag
    18721 cgcaggtact cttccagcaa gctaaccgac tgaaacttct tgataaacag gttaaatgct
    18781 tctgtacgat caattttgat atccagatcc agcacacgaa gcttagtgaa tgttttctca
    18841 agcgcctgcc actgaacagg ttctggaggc gtgacaaccg ccgcgctggt ccatttttgc
    18901 ggcaggatga aagagatcag taaacctgcg caggcaaacg caaaaacgac cgccatgacc
    18961 gtttttttgg cccgccataa aacttcaatt agattaagta aatcaacttc attattactg
    19021 ggcgacgcca gaggatactc gggaaaatga gcttcacttc cctgtttaat attcagtgat
    19081 gacatggggt ataacctgaa aatgagtcca ataagagtaa atctccacgg cccccactct
    19141 accagttggt gagatttatc cgaagctgaa gtttgtaaat tactcccact gaatgcaaca
    19201 ttcactctat ttcagagaaa tacccaccaa atttataaat taaataaatg ataaacccat
    19261 aattatttcc tgtaattatg ggtttataga taatattaat ttacctgttt agcgtagcgc
    19321 gaataatcgg cccaattttt tcaaacgccc ctggagagat aatatccaca tgcgcacaat
    19381 cctgacgata gatatccagc tcggctatcc acggcgacca ggcgcgttcg ggattcatac
    19441 cttcctgaag cgcgcgttca gcaacaaaca gcgtcgcttt accgtcaaac ggtacactat
    19501 gagcagtcgt cagcaggcgc acagcatcag cgtagttgcc ttcaatggtg gtaaacaact
    19561 ccgttgaagt acttccctgc tgtgccgcca ggaaggcctc gcgctcgcgg ttaatctccg
    19621 ccagcacttc cgggtccaga ccattggctt ctttttcctg ccagttttgc gtttctggcg
    19681 gccaggtatc cagcaagcca agaaatgcca cctgttcgcc acgggcacgc agtcgcgccg
    19741 caatgccctg cgccagcgta ccgccaagtg aataacccag caggtaataa ggaccgtgcg
    19801 gttgttgttc aagtaacgtt gccagatgcg cttcgcagac ttcatccagg tttgccgccg
    19861 tctgcatggg gccatgaggg cgcggcgact gaatgccgat aatcgaccat tgtgaatcga
    19921 gataacgcga gagcacgcta aactgccagg caaaaccgga ggccggatgg aaacaaaaca
    19981 gcgtcgggcc attaccttca cgcaacggca gaatggtttc gaatcccatg cgctgggtgc
    20041 tgtcttcttc accatcaata atcgttgcca gtttggcgac agttgacgcg accatcacct
    20101 gccccggcgt cacctgacga acaaactgtc gacttaactg cgctgccagt ttcattgcca
    20161 gtagcgaatg accgccaagc gcaaagaaat cagcatcggc atcctgcacg tcacaaccca
    20221 gcaacgacga gaatgccgcg gcgataatcg tttcactgcc cgctttcggc gcacgccccg
    20281 gcgcttgtgc tttcagttcc ggcaacggta aggctttgcg atccagcttg ccgttggcgc
    20341 taagaggtaa ctgtggaagt tgcagcagaa ccacgggcac catatgtggt ggcaatgttt
    20401 cgcgaagctg agcctgtaat gcgctggtat ccaacggcag gcccgattgc gacaccagat
    20461 agcccaccaa ctgacgcgca tcaccaccag tggctgccgc ctggttaatc acacaggcgt
    20521 gggtaacggc ttgttcgaca tccggcagcg cctgcatcac gcgatcgatt tcgcccagtt
    20581 cgatacgctg cccgcgaatt tttagctgat cgtcactgcg cccgaggtac tccaccgcgc
    20641 cgttatccag ccagcgggca acgtctccgg tacggtacat ccgttcgcca ggggcaaaag
    20701 gatcggcaat aaagcggctg gcggtcagat cgggtcgtcc aagatacccc tgcgccagtt
    20761 gaataccggt gagatagaga tctcccgcca cacccggcgg caccggatgc atcatcgcat
    20821 caagaatacg caggcccgta ttccacaccg gataaccaat cggcacactg ctgccgcgca
    20881 cctgtgccag ttcctcgcca aaagccggat accagctgac atctaccgcc gcttccgtcg
    20941 ggccatatag attatgcaac ggcacgctgg ttaactgttg ccattcgcgg cataaatcag
    21001 cgggtaaggc ctcaccacta cagaaaacct gtttcaacgt cgcgcaattc tggcgagcgg
    21061 tttgcggagt cagcgatgca acaaatgccg ccagcatcga cggcacaaag tgcgtggtcg
    21121 ttacaccata ttcggcaaag aattgctgca tagcgagcgg gtcgcggtgc gcttccggtt
    21181 cagccatcac cagtttagcc ccggcaataa acggccagaa aaactcccac accgagacat
    21241 caaaactgca cggcgttttt tgggcaacga catcttcgcc tgtaagcggg tagtggtttt
    21301 gcatccaaag cagacggtta acgatagccg tctgcccgac cattacccct ttcggcctgc
    21361 cggtggagcc agaggtaaag atgatataag ccgtgtggtg cggttgtgaa agtttcagcg
    21421 gcgcactgcc ctgcggtgta agcggggcgt tatagcaaag gctggttaaa tctggaacat
    21481 cggcaaagcg cggcagttga tcgtcggtgg tgattaacag cgacggacgc gcatcttcca
    21541 gcatcatttt caggcgatcg tccggatagc cggtatccag cggtaaccag gccgcaccag
    21601 cctcaacaat tgcatgtagc gccagagtca aaaagaccga gcgcggtagc gccaccgcca
    21661 cgctgtcgcc tggtttaacg ccgcgctcac gcagcagatt cgccagcgcc accacctgct
    21721 cgcgcatttc ccgatagctg aacaggtaac gcgcatctgc cagcgccgga gcatgcggtg
    21781 tttttgccgc ttgttctgcc accagcgcgc tcagcgtggt ttctggaatc tcaacctgag
    21841 tggcgttgat ctgcgccagc tgcgcatatt cacctggcag cataatatcg acatcgccgc
    21901 acaacagcgc aggatccgcg gcgaactggg caatcagcat tttcaggcgt tcagcatgct
    21961 ggattaacgt tggctcagcg taacgctgtt tattggcgag gatttcaata ctcaaatctc
    22021 cgtgttcatc cgggaacagg gccagttcaa ggtcattaac cggaccggtt gccagggtat
    22081 gggtttgcgc ctgaacacca ggaatatcca gttggtaatc aaataccttg atattgagta
    22141 ccggaccgaa cagcggttcc tctcctgccg ctcgcccact gtcacggaca atttgttcgg
    22201 cgtcgtagcg ttgatggcgg cgcatttttt tcagttgtgc tgccagtcgg gttgccagtt
    22261 gcggcagcgt ttcttgcgcc gcaatgtgaa tacccaacgg taaaacgttg agcacgggtc
    22321 cggtagccgt cagcgccgcc gagcccagtc gacgcataaa gataaatccg gcagcgtagt
    22381 ccatgcggtt gcacaatcgc cccagccaca aagccgtcag cgcaagggct aaatcggtac
    22441 gctgcacacc tgaaagttgc gtagccagct ggcggaattc cccgtcggta aattccagtt
    22501 tcaggcgcaa aatatctgcc gaagcgctgc gccccggtaa aggtgccgga gaaagtgacg
    22561 cgggcggtgg cagttgacga cgctgttccg cccagaatgc cgcatcacgc tgccaggctt
    22621 cgctttcgcg gtattgctgg tactcttcca ctacatcagc gaaaggcgta aacggcgaag
    22681 caggcgttgg ttcgccacgc agccatgcgc agtaaatatt ggcgatctgg cgggtaatgg
    22741 ccgggaaact gaagccatcg accagcaaat ggtgataacg ctgataccag taccagcggt
    22801 tatccgccac ctgtatcaac tgatgaaaga ccagtggttt accgctatcg acgcgcagat
    22861 cttgttgcaa atccgcctgc attaatgact gcgcagtacc gtgcggatca atgttggttc
    22921 gcaggtcgat agtttctggc agttcgaacg tcagcgcatc atcgacccac tgccagactt
    22981 cgccgttatc ttccgtaaaa cgcatccgca gcgtatctgc ttgcgctaat ccggcaacca
    23041 ccgcgcgggc cagtaatggc gcatcaacct ctccggttaa ctcaacgtaa tgcgccacgc
    23101 tccaggcgga gggtaaatcc gacagttttt ctgccatcca gatgccgggc tgtgcggcga
    23161 ccaaaggtaa atgctggctc attgtgcctc ctgcaactgg gtgaaattcg ccggtgtcag
    23221 agtacgccag tgggcttcca gccactgctg gcaggacacc tgtgactgcg gctgacacac
    23281 aacgtcccag cctgccggta aggcgcattg ttgcggccac agactgaatt gcccctgcgc
    23341 attgcgcaaa atgtaaaacg ctccctgcgg atcatcgaag ggattactga atgccatatt
    23401 caactcctgt catggaaaag tggttgccag aggtcgatta gcccctgcat caagccaccg
    23461 cgccaacaaa gcgcatcatg tccgccgtca acctgacgcc agaaaatgga ttcttttatg
    23521 gggtgtaatt gcgcatacag cgcctgattg gcctgcatga tcatcggctc gcgaataccc
    23581 gcttccagca caatgcgcag accttcggca ctaacttgac cagctttaag ttgctcaagt
    23641 aaaacacctt cttgctgccc gccccgatgc ggccaccagt acgatcctga ctggcttaat
    23701 acacagccaa agcgttcagg ccagtgcagt ccggcataca gcgcggaaag cccaccaaaa
    23761 ctctgcccgg cgaccacggt gcgatcggcg cgatcgctaa aaggggcaat agctttcacc
    23821 aggggtaata actcttgctg tactgccagc cagaaatccg cattacacgg cagttcgtgg
    23881 gcgcggtgcg tggtgtcaat agcgtcgatc aacacataca cggctggagg aagttgctga
    23941 cgatgggtca acgaagtcag cacaggccag acaggcatac tctgcgccca aaattcgcca
    24001 tcgagcaaaa ctgccagcgg acgttctcct gctgttgcat cgccagtggt aaaaatccat
    24061 acacggcgtg aatttttcaa ccgctcactt ttccagataa tttctttggc aggtgtttct
    24121 ggcgcttgcg gacaatccca tcccggttgt aggggcgctt gcggcatttc gagtgcagaa
    24181 acagcgtgcc ctcgcccgcc tttccagctt tgtggattca gcggatcggc tatcgcctgg
    24241 ggtaatagtt ttcgccagcc ttcacgtaat tcgaggcgat cgggggatgg tgcagaaaaa
    24301 atgtcatcgc gttcggtggg aataaaacag tagctgccgc gccagttggc attaagttgt
    24361 gtcgtccact gccagacgtt ggtgcctgca attcgctgca tcgactgggg ctggctgttc
    24421 tgatggtgat cggtcacacc agtgatgtag acccatacgc gctttatcgt cgagtattct
    24481 tcagaaccct ggggatcacg ccaccggaaa gtgacctcaa acatttcgtc attcagacgc
    24541 tgccattccg ggccatgttt cgactgccac cagctctcac ttcctacttt taacgccgtc
    24601 acaccataac cccatgttta ctgtgcaatt tttcattgat tgcagaaata tattgataat
    24661 attattgata actatttgca tttgcaatag cgtaatggcg cgccgtggga agcgcggaca
    24721 ttaattaacc aactgcactg cgtgtctttc aggatcaaag gttttcgcgg tagcgggatg
    24781 cgtcgtgttg atgacgacca tgcccgacag ttgcaattcg tggcaaaaat gcaggaataa
    24841 aacaatgaac aagaagattc attccctggc cttgttggtc aatctgggga tttatggggt
    24901 agcgcaggca caagagccga ccgatactcc tgtttcacat gacgatacca ttgtcgttac
    24961 cgccgccgag cagaacttgc aggcacctgg cgtttcgacc atcaccgcag atgaaatccg
    25021 caaaaacccg gttgcccgcg atgtgtcgga gatcatccgt accatgccag gcgttaacct
    25081 gaccggtaac tccaccagtg gtcagcgtgg gaataaccgc cagattgata ttcgcggtat
    25141 gggtccggaa aacacgctga ttttgattga cggcaagccg gtaagcagcc gtaactcggt
    25201 gcgtcagggc tggcgtggcg agcgcgatac ccgtggtgat acctcctggg tgccacctga
    25261 aatgattgaa cgtattgaag ttctgcgtgg tccggcagct gcgcgttatg gcaacggcgc
    25321 ggcgggcggc gtggttaaca tcattaccaa aaaaggcagt ggtgagtggc acggctcgtg
    25381 ggacgcttat ttcaatgcgc cagaacataa agaggaaggt gccaccaaac gcactaactt
    25441 tagcctcacc ggtccgctgg gcgacgaatt cagcttccgc ttgtatggca acctcgacaa
    25501 aactcaggct gacgcgtggg atatcaacca gggccatcag tccgcgcgtg ccggaacgta
    25561 tgccacgacg ttaccagccg ggcgcgaagg ggtgatcaac aaagatatta acggcgtggt
    25621 gcgctgggac ttcgcaccat tgcaatcgct ggaactggaa gcgggttaca gccgccaggg
    25681 taacctgtat gcgggcgata cccagaacac caactctgac tcctataccc gctcgaaata
    25741 tggcgatgaa accaaccgtc tgtatcgcca gaactactcg ctgacatgga acggcggctg
    25801 ggataacggc gtgaccacca gcaactgggt gcagtacgaa cacacccgta actcgcgtat
    25861 tccggaaggt ctggcgggcg gtaccgaagg gaaatttaac gaaaaagcga cacaggattt
    25921 cgtcgatatc gatctcgatg acgtgatgct gcacagcgaa gttaacctgc cgattgattt
    25981 cctcgttaac cagacgctga cgctgggtac ggagtggaat cagcagcgga tgaaggactt
    26041 gagttccaac acccaagcgc tcaccgggac gaataccggc ggcgctattg atggcgtgag
    26101 tgccaccgac cgtagcccgt attcaaaagc agaaattttc tcgctgtttg ccgaaaacaa
    26161 tatggagcta actgacagca ccatcgtaac gccggggctg cgtttcgatc atcacagtat
    26221 tgtcggcaat aactggagtc cggcgctgaa catatcgcaa ggtttaggcg atgacttcac
    26281 gctgaaaatg ggcattgctc gcgcttataa agcgccgagt ctgtaccaga ctaacccaaa
    26341 ctacattctc tacagtaaag gtcagggctg ctatgccagc gcgggcggct gctatctgca
    26401 aggtaatgat gacctgaaag cggaaaccag catcaacaaa gagattggtc tggagttcaa
    26461 acgcgacggt tggctggcgg gcgtcacctg gttccgtaac gattatcgca ataagattga
    26521 agcgggctat gtggctgtag ggcaaaacgc agtcggcacc gatctctatc agtgggataa
    26581 cgtaccgaaa gcggtggttg aaggtctgga aggatcgtta aacgtaccgg ttagcgaaac
    26641 ggtgatgtgg accaataaca tcacttatat gctgaagagt gaaaacaaaa ccacgggcga
    26701 ccgtttgtcg atcatcccgg agtatacgtt gaactcaacg ctgagctggc aggcacggga
    26761 agatttgtcg atgcaaacaa ccttcacctg gtacggcaag cagcagccga agaagtacaa
    26821 ctataaaggt cagccagcgg ttggaccgga aaccaaagaa atcagtcctt acagcattgt
    26881 gggtctgagc gcgacctggg atgtgacgaa gaatgtcagt ctgaccggcg gcgtggacaa
//
LOCUS       DAZKJR010000066        26603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKJR010000066 DAZKJR010000000
VERSION     DAZKJR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.066"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            116..1585
                     /gene="gspA"
                     /locus_tag="ACHOWS_002725"
     CDS             116..1585
                     /gene="gspA"
                     /locus_tag="ACHOWS_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFQ0048274.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSSQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTSVFT
                     AKHWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTDPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHQWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1587..2006
                     /gene="gspB"
                     /locus_tag="ACHOWS_002726"
     CDS             1587..2006
                     /gene="gspB"
                     /locus_tag="ACHOWS_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFQ0048275.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2244..2555
                     /gene="rpsJ"
                     /locus_tag="ACHOWS_002727"
     CDS             2244..2555
                     /gene="rpsJ"
                     /locus_tag="ACHOWS_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0048276.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2588..3217
                     /gene="rplC"
                     /locus_tag="ACHOWS_002728"
     CDS             2588..3217
                     /gene="rplC"
                     /locus_tag="ACHOWS_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0048277.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3228..3833
                     /gene="rplD"
                     /locus_tag="ACHOWS_002729"
     CDS             3228..3833
                     /gene="rplD"
                     /locus_tag="ACHOWS_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0048278.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3830..4132
                     /gene="rplW"
                     /locus_tag="ACHOWS_002730"
     CDS             3830..4132
                     /gene="rplW"
                     /locus_tag="ACHOWS_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0048279.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4150..4971
                     /gene="rplB"
                     /locus_tag="ACHOWS_002731"
     CDS             4150..4971
                     /gene="rplB"
                     /locus_tag="ACHOWS_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0048280.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4988..5266
                     /gene="rpsS"
                     /locus_tag="ACHOWS_002732"
     CDS             4988..5266
                     /gene="rpsS"
                     /locus_tag="ACHOWS_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0048281.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5281..5613
                     /gene="rplV"
                     /locus_tag="ACHOWS_002733"
     CDS             5281..5613
                     /gene="rplV"
                     /locus_tag="ACHOWS_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0048282.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5631..6332
                     /gene="rpsC"
                     /locus_tag="ACHOWS_002734"
     CDS             5631..6332
                     /gene="rpsC"
                     /locus_tag="ACHOWS_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0048283.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6345..6755
                     /gene="rplP"
                     /locus_tag="ACHOWS_002735"
     CDS             6345..6755
                     /gene="rplP"
                     /locus_tag="ACHOWS_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0048284.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6755..6946
                     /gene="rpmC"
                     /locus_tag="ACHOWS_002736"
     CDS             6755..6946
                     /gene="rpmC"
                     /locus_tag="ACHOWS_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0048285.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6946..7200
                     /gene="rpsQ"
                     /locus_tag="ACHOWS_002737"
     CDS             6946..7200
                     /gene="rpsQ"
                     /locus_tag="ACHOWS_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0048286.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7365..7736
                     /gene="rplN"
                     /locus_tag="ACHOWS_002738"
     CDS             7365..7736
                     /gene="rplN"
                     /locus_tag="ACHOWS_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0048287.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7747..8061
                     /gene="rplX"
                     /locus_tag="ACHOWS_002739"
     CDS             7747..8061
                     /gene="rplX"
                     /locus_tag="ACHOWS_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0048288.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8076..8615
                     /gene="rplE"
                     /locus_tag="ACHOWS_002740"
     CDS             8076..8615
                     /gene="rplE"
                     /locus_tag="ACHOWS_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0048289.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8630..8935
                     /gene="rpsN"
                     /locus_tag="ACHOWS_002741"
     CDS             8630..8935
                     /gene="rpsN"
                     /locus_tag="ACHOWS_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0048290.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8969..9361
                     /gene="rpsH"
                     /locus_tag="ACHOWS_002742"
     CDS             8969..9361
                     /gene="rpsH"
                     /locus_tag="ACHOWS_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0048291.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9374..9907
                     /gene="rplF"
                     /locus_tag="ACHOWS_002743"
     CDS             9374..9907
                     /gene="rplF"
                     /locus_tag="ACHOWS_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0048292.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9917..10270
                     /gene="rplR"
                     /locus_tag="ACHOWS_002744"
     CDS             9917..10270
                     /gene="rplR"
                     /locus_tag="ACHOWS_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0048293.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10285..10788
                     /gene="rpsE"
                     /locus_tag="ACHOWS_002745"
     CDS             10285..10788
                     /gene="rpsE"
                     /locus_tag="ACHOWS_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0048294.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10792..10971
                     /gene="rpmD"
                     /locus_tag="ACHOWS_002746"
     CDS             10792..10971
                     /gene="rpmD"
                     /locus_tag="ACHOWS_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0048295.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10975..11409
                     /gene="rplO"
                     /locus_tag="ACHOWS_002747"
     CDS             10975..11409
                     /gene="rplO"
                     /locus_tag="ACHOWS_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0048296.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11417..12748
                     /gene="secY"
                     /locus_tag="ACHOWS_002748"
     CDS             11417..12748
                     /gene="secY"
                     /locus_tag="ACHOWS_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0048297.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12780..12896
                     /gene="rpmJ"
                     /locus_tag="ACHOWS_002749"
     CDS             12780..12896
                     /gene="rpmJ"
                     /locus_tag="ACHOWS_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0048298.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13043..13399
                     /gene="rpsM"
                     /locus_tag="ACHOWS_002750"
     CDS             13043..13399
                     /gene="rpsM"
                     /locus_tag="ACHOWS_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0048299.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13416..13805
                     /gene="rpsK"
                     /locus_tag="ACHOWS_002751"
     CDS             13416..13805
                     /gene="rpsK"
                     /locus_tag="ACHOWS_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0048300.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13839..14459
                     /gene="rpsD"
                     /locus_tag="ACHOWS_002752"
     CDS             13839..14459
                     /gene="rpsD"
                     /locus_tag="ACHOWS_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0048301.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14485..15474
                     /gene="rpoA"
                     /locus_tag="ACHOWS_002753"
     CDS             14485..15474
                     /gene="rpoA"
                     /locus_tag="ACHOWS_002753"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0048302.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15515..15898
                     /gene="rplQ"
                     /locus_tag="ACHOWS_002754"
     CDS             15515..15898
                     /gene="rplQ"
                     /locus_tag="ACHOWS_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0048303.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            16005..16373
                     /locus_tag="ACHOWS_002755"
     CDS             16005..16373
                     /locus_tag="ACHOWS_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFQ0048304.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILADDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16384..16809
                     /gene="zntR"
                     /locus_tag="ACHOWS_002756"
     CDS             16384..16809
                     /gene="zntR"
                     /locus_tag="ACHOWS_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFQ0048305.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16865..17083
                     /gene="arfA"
                     /locus_tag="ACHOWS_002757"
     CDS             16865..17083
                     /gene="arfA"
                     /locus_tag="ACHOWS_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFQ0048306.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(17080..17490)
                     /gene="mscL"
                     /locus_tag="ACHOWS_002758"
     CDS             complement(17080..17490)
                     /gene="mscL"
                     /locus_tag="ACHOWS_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0048307.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPTPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17620..18996)
                     /gene="trkA"
                     /locus_tag="ACHOWS_002759"
     CDS             complement(17620..18996)
                     /gene="trkA"
                     /locus_tag="ACHOWS_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFQ0048308.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(19018..20307)
                     /gene="rsmB"
                     /locus_tag="ACHOWS_002760"
     CDS             complement(19018..20307)
                     /gene="rsmB"
                     /locus_tag="ACHOWS_002760"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0048309.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLNWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPPADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20353..21300)
                     /gene="fmt"
                     /locus_tag="ACHOWS_002761"
     CDS             complement(20353..21300)
                     /gene="fmt"
                     /locus_tag="ACHOWS_002761"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0048310.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21315..21824)
                     /gene="def"
                     /locus_tag="ACHOWS_002762"
     CDS             complement(21315..21824)
                     /gene="def"
                     /locus_tag="ACHOWS_002762"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0048311.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21954..23078
                     /gene="dprA"
                     /locus_tag="ACHOWS_002763"
     CDS             21954..23078
                     /gene="dprA"
                     /locus_tag="ACHOWS_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFQ0048312.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23050..23523
                     /gene="smg"
                     /locus_tag="ACHOWS_002764"
     CDS             23050..23523
                     /gene="smg"
                     /locus_tag="ACHOWS_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFQ0048313.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23552..24094
                     /locus_tag="ACHOWS_002765"
     CDS             23552..24094
                     /locus_tag="ACHOWS_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFQ0048314.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24099..24671
                     /gene="tsaC"
                     /locus_tag="ACHOWS_002766"
     CDS             24099..24671
                     /gene="tsaC"
                     /locus_tag="ACHOWS_002766"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFQ0048315.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24676..25494
                     /gene="aroE"
                     /locus_tag="ACHOWS_002767"
     CDS             24676..25494
                     /gene="aroE"
                     /locus_tag="ACHOWS_002767"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0048316.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25491..25748
                     /locus_tag="ACHOWS_002768"
     CDS             25491..25748
                     /locus_tag="ACHOWS_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFQ0048317.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25724..26278)
                     /locus_tag="ACHOWS_002769"
     CDS             complement(25724..26278)
                     /locus_tag="ACHOWS_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0048318.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSAKNYVKWKDEYLDQGNQTQP"
     gene            complement(26322..>26603)
                     /locus_tag="ACHOWS_002770"
     CDS             complement(26322..>26603)
                     /locus_tag="ACHOWS_002770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048319.1"
                     /translation="ATRLSAHCREVAYITLSSFRVNPEFQDFFSLLLRPSCEVFHVVV
                     STEAHYRDPNFLHKYFFDLFFCLLLFHPFCCIRTQNGAFLHAKRLAQGQ"
BASE COUNT         6783 a         6280 c         6945 g         6595 t
ORIGIN      
        1 tgccgcttgc atgaatcaag caaaatttat ttatattagt aatatgtatt taattaataa
       61 atgcatacat tatttttatg attaaggata atcaataatt aaaacaggaa gttctatgtc
      121 tacgagaaga gaagttattc tttcctggtt gcgtgagaaa cgtcagacct ggcgtctgtg
      181 ctatttgttg ggtgaggctg gaagtggaaa aacctggctg gcgcagcaac tacaaaaaga
      241 taaacatcgc cgtgtgatta ctttaagcct cgttgtttcc tcgcaaggta aggccgcatg
      301 gatcgttacc gacgataatg cggctgaaca gggctgccgt gacagcgcct ggacgcgaga
      361 tgagatggcg ggtcaattac tgcatgcgct tcatcgaact gatagtcgct gccctcttat
      421 tattattgaa aatgctcacc tgaatcaccg ccgcatactt gatgatctcc aacgcgcgat
      481 atctcttatt cctgacggtc aatttttgct gatcggcaga cccgatcgaa aagtcgaacg
      541 tgattttaaa aaacagggca ttgaacttgt ctcaacagga cgtctgatgg agcacgagct
      601 taaggcaagc atccttgaag gtcagaatat tgaccagccc gatctccttt taactgccag
      661 agttctgaaa cggatagctt tattatgtcg gggagatcgc agaaagctgg cgcttgccgg
      721 cgaaacaatt agcttattac agcaagccga gcagaccagc gttttcactg caaaacattg
      781 gcgaatgatt taccgcgtat taggtgataa ccgcccccgg aaaatgcagc ttgccgtggt
      841 gatgagtgga accattatcg cacttacctg tggttggcta ctgctgtcct ctttcacaga
      901 tccgttcccc gttcctgcgt ggctggtacc cgtaacgcca gtagttaaac aagatatgac
      961 taaggatatt gctcatgtag tgatgcgcga tagtgaggcg ctaagcgtgt tatacggcgt
     1021 atgggggtac gaggtcccag ctgattcggc ctggtgcgat caggcagtaa gagcggggct
     1081 ggcatgtaaa agtgggaatg cttctttaca aacattggta gatcagaatt tgccctggat
     1141 cgcatcgctt aaagtcgggg acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgatgc
     1201 taccgttgat gtcctcgtag ggcagcaaac ctggacgctg acacatcaat ggtttgagtc
     1261 ggtatggacg ggtgattatc ttctgttatg gaaaatgtcg ccggaagggg agagtaccat
     1321 tacgcgtgat agcagcgagg aagagattct ctggctggaa acgatgttaa accgcgcatt
     1381 gcatatttcg acagaacctt cggcagagtg gcgtcccttg ttggtagaaa aaataaagca
     1441 atttcaaaaa agccaccact tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca
     1501 tctctggcag gtggcagggg agagtgccta cttatatcgg gatgaagcaa atatttcccc
     1561 ggaaacaaca gtgaagggga aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga
     1621 aagaaacagg tcaccctggt atcttctccc gacaaaaaca cagcaccata atatatgtca
     1681 tttgtttatt gctgatttgc ctgtggtttg caggaatggt gttggttggt gggtatgcca
     1741 ggcagctatg ggcgctttgg atagtaaaag cagaagtcac tgtagaggct gaaacgcccg
     1801 cttttaaaca atcaacacaa cactatttct tcaaaaaaca gccactcccc gtcgtcgaat
     1861 ccgtcgagga ggaagatgac ccagacgtcg cggttgagaa tgctccctct tcttctgaag
     1921 acgaagaaaa tactgtagaa gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa
     1981 atgcgctgaa cgaacttgag agataacccg aaggctgttt actttactag caatacgctt
     2041 gcgttcggtg gttaagtatg tataatgcgc gggcttgtcg tagttgacag caggttcaat
     2101 ctgaacctca gttagccgaa tttggctacc taacaatgct cccaatcggg gagctacgta
     2161 agaacggtta cactctccca tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat
     2221 aaaataattg gagctctggt ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt
     2281 ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt cgagactgcc aagcgcactg
     2341 gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa agagcgcttc actgttctga
     2401 tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga aatccgtact cacttgcgtc
     2461 tggttgacat cgttgagcca accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg
     2521 ctgccggtgt agacgtgcag atcagcctgg gttaatcagg tcattgagcg attgagaggt
     2581 tgaaacaatg attggtttag tcggtaaaaa agtgggtatg acccgtatct tcacagaaga
     2641 cggcgtttct atcccagtaa ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa
     2701 agacctggct aacgatggct accgtgctat tcaggtaacc accggtgcta aaaaagctaa
     2761 ccgtgtgacc aagcctgaag ctggccactt cgctaaagct ggcgtagaag ctggccgtgg
     2821 tctgtgggaa ttccgcctgg ctgaaggcga agagttcact gtaggtcaga gcattagcgt
     2881 tgaactgttt gctgacgtta aaaaagttga cgtaactggc acctctaaag gtaaaggttt
     2941 cgcaggtacc gttaagcgct ggaacttccg tacccaggac gctactcacg gtaactcctt
     3001 gtctcaccgc gttccgggtt ctatcggtca gaaccagact ccgggcaaag tgttcaaagg
     3061 caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt
     3121 acgcgttgac gctgagcgca acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg
     3181 tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga gatagcaatg gaattagtat
     3241 tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac cttcggtcgt gatttcaacg
     3301 aagcgctggt tcaccaggtt gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg
     3361 ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca
     3421 ccggccgtgc gcgttctggt tctatcaaga gcccgatctg gcgttctggt ggcgtgacct
     3481 ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc
     3541 tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct gatcgttgtc gagaagttct
     3601 ctgtagaagc gccgaaaact aagctgctgg cacagaaact gaaagacatg gctctggaag
     3661 atgtgctgat catcaccggt gagctggacg aaaacctgtt cctggctgcg cgcaacctgc
     3721 acaaggttga cgtacgcgat gcaactggta tcgacccggt tagcctgatc gccttcgaca
     3781 aagtcgtaat gactgctgat gctgttaagc aagttgagga gatgctggca tgattcgtga
     3841 agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat
     3901 ggaaaaatct aacaccatcg tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa
     3961 agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa
     4021 agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt agcgactgga aaaaagctta
     4081 cgtcaccctg aaagaaggcc agaatctgga cttcgttggc ggcgctgagt aagtcggagg
     4141 agtaatacaa tggcagttgt taaatgtaaa ccgacatctc cgggtcgtcg ccacgttgtt
     4201 aaagtggtta accctgagct gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac
     4261 agcaaatccg gtggtcgtaa caacaatggc cgtatcacca ctcgtcatat cggtggtggc
     4321 cacaagcagg cttaccgtat tgttgacttc aaacgcaaca aagacggtat cccggcagtt
     4381 gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca tcgcgctggt tttgtacaaa
     4441 gacggtgaac gccgttacat cctggcccct aaaggcctga aagctggcga ccagattcag
     4501 tctggcgttg atgctgcaat caaaccaggt aacaccctgc cgatgcgcaa catcccggtt
     4561 ggttctactg ttcataacgt agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc
     4621 gctggtactt acgttcagat cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt
     4681 tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa ctctgggcga agttggcaat
     4741 gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt
     4801 ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc cacatggtgg tggtgaaggt
     4861 cgtaactttg gtaagcaccc ggtaactccg tggggcgttc agaccaaagg taagaagacc
     4921 cgcagcaaca agcgtactga taaattcatc gtacgtcgcc gtagcaaata attttagagg
     4981 ataagccatg ccacgttctc tcaagaaagg tccttttatt gacctgcact tgctgaagaa
     5041 ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg cgcacttggt cccgtcgttc
     5101 aacgatcttt cctaacatga tcggtttgac catcgctgtc cataatggtc gtcagcacgt
     5161 tccggtattt gtaaccgacg aaatggtcgg tcacaaactg ggtgaattcg caccgactcg
     5221 tacttatcgc ggccacgctg ctgataaaaa agcgaagaag aaataaggta ggaggaagag
     5281 atggaaacta tcgctaaaca tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt
     5341 gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg atattttgac ctacaccaac
     5401 aagaaagcgg ctgtactggt caagaaagtt ctggaatctg ccattgctaa cgctgaacac
     5461 aacgatggcg ctgacattga cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg
     5521 agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc
     5581 agccacatca ctgtggttgt gtccgatcgc tgagactctg gagactagca atgggtcaga
     5641 aagtacatcc taatggtatt cgcctgggta ttgtaaaacc atggaactct acttggtttg
     5701 cgaacaccaa agaattcgct gacaacctgg acagcgattt taaagtacgt cagtacctga
     5761 ctaaggaact ggctaaagcg tccgtatctc gtatcgttat cgagcgtccg gctaagagca
     5821 tccgtgtaac cattcacact gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg
     5881 tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt tcctgcacag atcaacatcg
     5941 ccgaagttcg taagcctgaa ctggacgcaa aactggttgc tgacagcatc acttctcagc
     6001 tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc
     6061 tgggcgctaa aggtattaaa gttgaagtta gcggccgtct gggcggcgca gaaatcgcac
     6121 gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact
     6181 acaacacctc tgaagcgcac accacttacg gtgtaatcgg cgttaaagtg tggatcttca
     6241 aaggcgagat cctgggtggt atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc
     6301 ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc
     6361 gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg
     6421 ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga
     6481 tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg tcaaggtaag atctggatcc
     6541 gtgtgttccc ggacaaaccg atcactgaaa agccgctggc agtgcgtatg ggtaaaggta
     6601 aaggtaacgt ggagtattgg gttgccttga ttcagccggg taaagtcctg tatgaaatgg
     6661 acggtgttcc ggaagagctg gcccgtgaag cattcaagct ggcagcagcg aaactgccga
     6721 ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa
     6781 gagcgttgaa gagctgaaca ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg
     6841 tatgcaggct gcaagtggcc agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga
     6901 tgtcgcacgc gttaagactt tactgaacga gaaggcgggt gcgtaatgac cgataaaatc
     6961 cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga aatccattgt tgttgctatc
     7021 gaacgttttg tgaaacaccc gatctacggt aaattcatca agcgtacgac caaactgcac
     7081 gtacatgacg agaacaacga atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt
     7141 ccgctgtcca agactaaatc ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa
     7201 tacagtacac tctctcgata cgaataaacg gctcagaaat gagccgttta ttttttctac
     7261 ccatatcctt gaagcggtgt tataatgccg cgccctcgat atggggattt ttaacgacct
     7321 gattttcggg tctcagtagt agttgacatt agcggagcac taaaatgatc caagaacaga
     7381 ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg
     7441 gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat caagatcacc atcaaagaag
     7501 caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca
     7561 agaagggtgt tcgtcgcccg gacggttctg tcattcgctt cgatggtaat gcttgtgttc
     7621 ttctgaacaa caacagcgag cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg
     7681 agcttcgtag tgagaagttc atgaaaatta tctctctggc accagaagta ctctaaggag
     7741 cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat
     7801 aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt
     7861 atcaacctgg ttaagaaaca tcagaagccg gttccggccc tgaaccaacc gggtggcatc
     7921 gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc
     7981 aaggctgacc gtgtaggctt tagattcgaa gacggtaaaa aagtccgttt cttcaagtct
     8041 aacagcgaaa ctatcaagta atttggagta gtacgatggc gaaactgcat gattactaca
     8101 aagacgaagt agttaaaaaa ctcatgactg agtttaacta caattctgtc atgcaagtcc
     8161 ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac
     8221 tgctggataa cgcagcagca gacctggcag caatctccgg tcaaaaaccg ctgatcacca
     8281 aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag
     8341 taactctgcg tggcgaacgc atgtgggagt tctttgagcg cctgatcact attgctgtac
     8401 ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca
     8461 gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg
     8521 ttcgtggttt ggatattacc attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc
     8581 tgctggctgc ctttgacttc ccgttccgca agtaaggtag ggttactaaa tggctaagca
     8641 atcaatgaaa gcacgcgaag taaaacgcgt agctttagct gataaatact tcgcgaaacg
     8701 cgctgaactg aaagcgatca tctctgatgt gaacgcttcc gacgaagatc gttggaacgc
     8761 tgttctcaag ctgcagactc tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg
     8821 ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc gggttgagcc gtattaaggt
     8881 ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc
     8941 accaattgaa tcacgggagt aaagacagat gagcatgcaa gatccgatcg cggatatgct
     9001 gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa
     9061 gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt tttattgaag attttaaagt
     9121 tgaaggcgac accaagcctg aactggaact tactctgaag tatttccagg gcaaagctgt
     9181 tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc atctataagc gtaaagatga
     9241 gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt tctacctcta aaggtgttat
     9301 gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa attatctgct acgtagccta
     9361 atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc gttgttcctg ccggcgttga
     9421 cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa aacggcgagc tgactcgtac
     9481 tctcaacgat gctgttgaag ttaaacatgc agataatacc ctgaccttcg gtccgcgtga
     9541 tggttacgca gacggttggg cacaggctgg taccgcgcgt gccctgctga actcaatggt
     9601 tatcggtgtt accgaaggct tcactaagaa gctgcagctg gttggtgtag gttaccgtgc
     9661 agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc tctcatcctg ttgaccatca
     9721 gctgcctgcg ggtatcactg ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgc
     9781 tgataagcag gtgatcggcc aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc
     9841 ttacaaaggc aagggtgttc gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa
     9901 gaagtaaggt aacactatgg ataagaaatc tgctcgtatc cgtcgtgcga cccgcgcacg
     9961 ccgcaagctc caggagctgg gcgcaactcg cctggtggta catcgtaccc cgcgtcacat
    10021 ttacgcacag gtaattgcac cgaacggttc tgaagttctg gtagctgctt ctactgtaga
    10081 aaaagctatc gctgaacaac tgaagtacac cggtaacaaa gacgcggctg cagctgtggg
    10141 taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc
    10201 cgggttccaa tatcatggtc gtgtccaggc actggcagat gctgcccgtg aagctggcct
    10261 tcagttctaa ggtagaggtg taagatggct cacatcgaaa aacaagctgg cgaactgcag
    10321 gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc
    10381 ttcacagctc tgactgtagt tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa
    10441 gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa aagcccgtcg caatatgatt
    10501 aacgtcgcgc tgaataacgg tactctgcaa caccctgtta aaggtgttca tacgggttct
    10561 cgcgtattca tgcagccggc ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc
    10621 gccgttctgg aagtcgctgg ggttcataac gttctggcta aagcgtatgg ttccaccaac
    10681 ccgattaacg tggttcgtgc aactatcgat ggcctggaaa atatgaattc cccagaaatg
    10741 gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg ggaaataaac catggcaaag
    10801 actattaaaa ttactcaaac ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg
    10861 ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag agcgcgagga tactcctgct
    10921 attcgcggta tgatcaacgc ggtttccttc atggttaaag ttgaggagta agagatgcgt
    10981 ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt
    11041 atcggttctg gcctcggtaa aaccggtggt cgtggtcaca aaggtcagaa atctcgttct
    11101 ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg
    11161 aaattcggct tcacttctcg taaagcagcg attacagccg aagttcgtct gtctgacctg
    11221 gctaaagtag aaggcggtgt tgtagacctg aacacgctga aagcggctaa cattatcggt
    11281 atccagatcg agttcgcgaa agtgatcctg gctggcgaag tcactactcc ggtaactgtt
    11341 cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc
    11401 gaggaataag tagcagatgg ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg
    11461 cttaggcgag ctgaaacgca gactgctgtt tgttatcggt gcgctgattg tgttccgtat
    11521 tggctctttt attccgatcc ctggtattga tgccgctgta cttgccaaac tgcttgagca
    11581 acagcgaggc accatcattg aaatgtttaa catgttctct ggtggtgctc tcagccgtgc
    11641 ttctatcttt gctctgggga tcatgccata tatttcggca tcgatcatta tccagcttct
    11701 gacggtggtt cacccaacgt tggcagaaat taagaaagaa ggggagtctg gtcgtcgtaa
    11761 gatcagccag tacacccgct acggtactct ggtgctggca atattccagt cgatcggtat
    11821 tgctaccggt ctgccgaata tgcctggtat gcaaggcctg gtcattaacc cgggctttgc
    11881 attctacttc accgctgttg taagtctggt cacaggaacc atgttcctga tgtggttggg
    11941 cgaacagatt actgaacgag gtatcggcaa cggtatttca atcattatct tcgccggtat
    12001 tgtcgcggga ctcccgccag ccattgccca tactatcgag caagcgcgtc aaggcgacct
    12061 gcacttcctc gtgttgctgt tggttgcagt attagtattt gcagtgacgt tctttgttgt
    12121 atttgttgag cgtggtcaac gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg
    12181 tcgtgtctat gctgcacaga gcacacattt accgctgaaa gtgaatatgg cgggggtaat
    12241 cccggcaatc ttcgcttcca gtattattct gttcccggcg accatcgcgt catggttcgg
    12301 gggcggtact ggttggaact ggctgacaac aatttcgctg tatttgcagc ctgggcaacc
    12361 gctttatgtg ttactctatg cgtctgcaat catcttcttc tgtttcttct acacggcgtt
    12421 ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag tccggtgcat ttgtaccagg
    12481 aattcgtccg ggagagcaaa cggcgaagta tatcgataaa gtaatgaccc gcctgaccct
    12541 ggttggtgcg ctgtacatta cctttatctg cctgatcccg gagttcatgc gtgatgcaat
    12601 gaaagtaccg ttctactttg gtgggacctc actgcttatc gttgttgtcg tgattatgga
    12661 ctttatggct caagtgcaaa ctctgatgat gtccagtcag tatgagtctg cattgaagaa
    12721 ggcgaacctg aaaggctacg gccgataatt ggtcgcccga gaagttacgg agagtaaaaa
    12781 tgaaagttcg tgcttccgtc aagaaattat gccgtaactg caaaatcgtt aagcgtgatg
    12841 gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa acagcgccaa ggctgatttt
    12901 ttcgcatatt tttcttgcaa agttgggttg agctggctag attagccagc caatcttttg
    12961 tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca
    13021 tattaaatag taggagtgca tagtggcccg tatagcaggc attaacattc ctgatcataa
    13081 gcatgccgta atcgcattaa cttcgattta tggcgtcggc aagacccgtt ctaaagccat
    13141 cctggctgca gcgggtatcg ctgaagatgt taagatcagt gagctgtctg aaggacaaat
    13201 cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat
    13261 cagcatgagc atcaagcgcc tgatggatct tggttgctat cgcggtttgc gtcatcgtcg
    13321 tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca cgtacccgta agggtccgcg
    13381 caaaccgatc aagaaataat cggggtgatt gaataatggc aaaggcacca attcgtgcac
    13441 gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca tatccatgct tctttcaaca
    13501 acaccatcgt gactatcact gatcgtcagg gtaacgcgtt gggttgggca acagccggtg
    13561 gttccggttt ccgtggttct cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc
    13621 gttgcgctga cgccgtgaaa gaatacggca tcaagaatct ggaagttatg gttaaaggtc
    13681 cgggtccagg ccgcgaatct actattcgtg ctctgaacgc cgcaggtttc cgcatcacta
    13741 acattactga tgtgactccg atccctcata acggttgtcg tccgccgaaa aaacgtcgcg
    13801 tataacgctt cgttttccag gtttgttgga gaaagaaaat ggcaagatat ttgggtccta
    13861 agctcaagct gagccgtcgt gagggcaccg acttattcct taagtctggc gttcgcgcga
    13921 tcgataccaa gtgtaaaatt gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc
    13981 tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc
    14041 tggagcgtca gttccgtaac tactacaaag aagcagcacg tctgaaaggc aacaccggtg
    14101 aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg
    14161 gtgccactcg tgcagaagca cgtcagctgg ttagccataa agcaattatg gtaaacggtc
    14221 gtgttgttaa catcgcttct tatcaggtta gtccgaatga cgttgtaagc attcgtgaga
    14281 aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc
    14341 caacctggct ggaagttgat gctggcaaga tggaaggtac gtttaagcgt aagccggagc
    14401 gttctgatct gtctgcggac attaacgaac acctgatcgt cgagctttac tccaagtaaa
    14461 gcttagtacc aaagagagga cacaatgcag ggttctgtga cagagtttct aaaaccgcgc
    14521 ctggttgata tcgagcaagt gagttcgacg cacgccaagg tgacccttga gcctttagag
    14581 cgtggctttg gccatactct gggtaacgca ctgcgccgta ttctgctctc atcgatgccg
    14641 ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac atgagtacag caccaaagaa
    14701 ggcgttcagg aagatatcct ggaaatcctg ctcaacctga aagggctggc ggtgagagtt
    14761 cagggcaaag atgaagttat tcttaccttg aataaatctg gcattggccc tgtgactgca
    14821 gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac
    14881 ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat
    14941 gtgccggctt ctacccgaat tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg
    15001 gtcgacgcat gctacagccc tgtggagcgt attgcctaca atgttgaagc agcgcgtgta
    15061 gaacagcgta ccgacctgga caagctggtc atcgaaatgg aaaccaacgg cacaatcgat
    15121 cctgaagagg cgattcgtcg tgcggcaacc attctggctg aacaactgga agctttcgtt
    15181 gacttacgtg atgtacgtca gcctgaagtg aaagaagaga aaccagagtt cgatccgatc
    15241 ctgctgcgcc ctgttgacga tctggaattg actgtccgct ctgctaactg ccttaaagca
    15301 gaagctatcc actatatcgg tgatctggta cagcgtaccg aggttgagct ccttaaaacg
    15361 cctaaccttg gtaaaaaatc tcttactgag attaaagacg tgctggcttc ccgtggactg
    15421 tctctgggca tgcgcctgga aaactggcca ccggcaagca tcgctgacga gtaaccggat
    15481 cacaggttaa ggttttactg agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca
    15541 actgaaccgc aacagcagcc atcgccaggc tatgttccgc aatatggcag gttcactggt
    15601 tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga
    15661 gccgctgatt actcttgcca agactgatag cgttgctaat cgtcgtctgg cattcgcccg
    15721 tactcgtgat aacgagatcg tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag
    15781 ccgtgccggt ggttacactc gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc
    15841 gatggcttac atcgagctgg ttgatcgttc agagaaagca gaagctgctg cagagtaatc
    15901 tgaagcaacg taaaaaaacc cgccccggcg ggttttttta tacccgtagc atccccactt
    15961 atctacaata gctgtactct ttttgttcat cccctggagt atatatgtgg ttacttgacc
    16021 agtgggcaga gcgtcatata gcagaagcgc aagcgaaagg tgagtttgat aacctggcag
    16081 gtagcggcga accattgata ctggctgatg attctcatgt gccaccggaa ttacgtgcgg
    16141 ggtatcgctt gctgaagaat gccggttgct taccgccaga acttgagcag cggagggaag
    16201 caattcagct tctggatatc ctgagaggta tccgtcacga tgatccgcaa tatcaagagg
    16261 ttagccgtcg attgtcatta ctggaattga agctgcgaca agctggactg agcaccgatt
    16321 ttttacgcgg cgattatgct gacaagttgt tgaataaaat caacgataac tagtggagta
    16381 tgtatgtatc gcattggtga gctggcaaaa atggcggaag taacacccga cacgattcgt
    16441 tattacgaaa aacagcagat gatggagcat gaagtgcgta ctgaaggtgg gtttcgcctg
    16501 tataccgaaa gcgatctcca gcgattgaaa tttatccgcc atgccagaca actaggtttc
    16561 agtctagagt cgatccgcga gttgctgtcg atccgtatcg atcctgaaca ccatacctgt
    16621 caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag
    16681 ttgcagagta tgcagcgttc cctccaacgc cttaacgatg cctgttgtgg gaccgcccat
    16741 agcagtgttt attgctcgat tcttgaagct cttgaacaag gggcgagtgg cgttaagagt
    16801 ggctgttgat tttttacact ggcaggatta cactcgcgcc gttaaataac caattggagt
    16861 ttttatgagc cgatatcagc atactaaagg gcagataaag gataatgcga tagaagcatt
    16921 actacatgat cccttattcc gacagcgcgt agagagaaat aagaaaggga aaggcagtta
    16981 catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag gccagtggca agaaagtaaa
    17041 tcactttttt accactggcc ttctgctttc aggcgcttgt taagagcggt tattctgctc
    17101 tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct ttagttggtg taggcgcggc
    17161 tgctggttct tcttttttcc gattcagttt gttgattagc ttaatcgcca taaagatggc
    17221 aaaggctaca atcagaaaat caaagacgtt ttgaatgaag acaccgtaat gcatcacaac
    17281 agcagggata tccccctgcg catcgcgtag cgtgacagca aactgtttga aatcgatccc
    17341 gccaattaat aaccccagag gtggcataat gatatcggca accagtgaag atacaatctt
    17401 cccgaatgcc gcaccgataa tgacacccac cgccaaatcc accacgttcc cgcgcatcgc
    17461 aaattcgcga aattctttaa taatgctcat gttattctcc ctatgaagcc gacaatcata
    17521 agtctaacaa atgttaagct attttcctgc caggagtgaa tttaattaat ccctgataat
    17581 caaagggaaa taataaggcg tcattagacg ccttattaat tacaagaaga aaggacttgg
    17641 ctggaagagt ctttcgacgt cggtaataaa ctttttatct gtgaggaaca taattacgtg
    17701 atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac
    17761 cgctccaata atcgttcctg gcggtagctt gatttcatca atgactctgc cgacaacgcg
    17821 tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct tctgctacgc cgcggcgcag
    17881 tgaggaaaca ccaacaatat cagcttttcg cacatggcta agcaacgcag aaatagttgc
    17941 ttgttgcggt gaaatcgcaa tatcgatcac gctcccctga accagatcca cataagcgcg
    18001 acgctggatc aataccatca cctttttcgc acccatacgt ttggcaagca tggcggacat
    18061 gatattggct tcgtcatcgt tggtgacagc aataaacaga tcaacttgat cgatatgttc
    18121 ttcggccagc agttcttgat ccgacgcatc accaaaaaag acgatcgtat tctgcaactt
    18181 ttccgccagt tcggcagcgc gctgctgatt acgttcgatg agtttaacgc tgtaatcttt
    18241 ttccagacga cgcgccagcc ctgcaccgat attaccgccg ccaaccagca tgatccgctt
    18301 atacggtttt tccagtcgct gtaattcact catcaccgcg cggatatgct gtgaagcggc
    18361 aataaagaac acttcatcac cagcttcaac aatggtcgaa ccttgcggac gaatggggcg
    18421 atcgtggcgg aaaattgctg ccacacgagt atcgatatgg ggcatatgtt cgcgcatggt
    18481 cgaaagtgca ttaccaatca gcgggccgcc ataataggct ttaaccacag ccaggctgac
    18541 tttaccctca gcgaagttca ccacctgcaa tgcgccggga tactcaatca gtcggtaaat
    18601 attatcgata accaactgct ctggtgcgat cagatgatca atcggtacag catctgaatg
    18661 aaatagttta tcggcatcgc gcacgtagtc tggtgagcgg atacgagcga tgcgattagg
    18721 ggtgttgaaa agtgagtagg ctacctggca ggcaaccata ttggtttcat ctgaactagt
    18781 tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga
    18841 gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc
    18901 gttggtatcg acaacagtaa tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc
    18961 gccaacctgg ccggcaccca gaatgataat tttcatcagt cgcgacccgt tatctcatca
    19021 ctttttgatt agcttagcgt aaaagaagcc gtcgccctct tcggcaccag gcagattttg
    19081 tttacccggt tgctctggtg ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa
    19141 aaaggcttta atctgcaggc tattctcttc cggtaacacc gaacaggtgg catagaccag
    19201 ggttccaccg gattttaaat gcggccaaat ggcgtcgaga atttcagact gcaattgcgc
    19261 gagttccggg atatcgcgat cgcgacgtaa ccatttaata tccggatggc gacgaatcac
    19321 accggttgct gaacaaggcg catctaataa aatgcgatca aactgttgct cgccacacca
    19381 ttgggaaggg tagcggccat caccttgttt cacggttgcc ttcataccaa ggcgttttaa
    19441 attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc
    19501 tggtgccacc tcaaggatat gcgttgtttt accgccgggg gcggcacaaa gatccaaaat
    19561 gtgttcaccg ttttgtggcg caagccaggt catgcaacct tgtgctgagg catcctgaac
    19621 ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca ggtgcaggtg tttccagacg
    19681 tacagcatca gggtaatccg caggcgggaa acctttcatt cctgcttcat ccagcaatgc
    19741 aagccagctg tcgcgggaat gatgcgtacg attaacgcgc agccacattg gcggacgctg
    19801 gttattggct tcgacgatgg attgccactg ctctggatac gctttttgca gacgcttcag
    19861 caaccaggaa ggatgcagat aacgtgcatc actggcatta aactcggcta ataactcttc
    19921 ttgctgacgc tggaactggc gtaatacacc gtttatcaac cctttaagtt gcgggcgctt
    19981 aattgcgata gcgccttcaa ccgtttcagc cagcgcagca tgaggtggta tgcgggtata
    20041 aagcagttga tacaaaccaa ccataatcag gtaatgcacg gtccgctgtt tgccggtcat
    20101 cggacgggcc attaacttat taatcagcca gtttaactgc gaaagcgtac gcagtacgcc
    20161 aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa actttttgct ggagcggtgg
    20221 cagaatgttg cttaatgatt gcccttgctc gacgacttgt tcaacggcct gggccgccat
    20281 gctacgtaaa ttacgttgtt ttttcataag tataaaaatg cccggcaaga ccgggcttag
    20341 aagagtggac tatcagacca gacggttgcc cggaacaaac cattcccgac gagagttcag
    20401 gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt aacgagagca ggttcaggat
    20461 gccatcacca gtcgcaacct gaatgccttg tttgttggct tcaaggatcg ttcctggtgc
    20521 agcgttggtt gccgtatcaa tgaccgatgc tttccagact ttaaccggct gtccttcaat
    20581 ttccagccag ctcattggcc atggattgaa agcgcgaatg cagcgttcaa gctgtgctgc
    20641 cgaaagtgac cagtcaatac gcgcttcttc tttactcaac ttctcggcgt aagtgacaag
    20701 agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc aattgtttca acgtggtgat
    20761 aagcccttgt gggccaagct ctgccagctt gtcgtacagc gtaccactgg tatcttctgc
    20821 agtaatcggg caggagagct tatagagcat gtctccggtg tctaaaccaa catccatttg
    20881 cataatggtc acaccagttt ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc
    20941 accgcgccag cgtggcagca gtgaaccatg aacgttgata cagccaagac gcggcatctc
    21001 cagcactgct ttaggcagaa ttaagccata ggcgacgaca accataacat cagcctgtag
    21061 atcggcgacc agttgctggt tttcttgtgg acgcagggaa accggttgaa aaacgggcag
    21121 acctttttcc tcagccagaa ctttaaccgg gctgggcatc agttttttac cgcgtcctgc
    21181 cggtcggtct ggctgggtga acacgccaac gacgttatga ccagaagaca acagcgcgtc
    21241 gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata atacgtagtg attctgacac
    21301 gttagttctt atccttaagc ccgggctttc agacgatcca gtttttcaac tttctgacga
    21361 atacgttgtt gtttcagcgg tgacagataa tccataaaca gtttgccgac caggtgatcc
    21421 atctcatgct gaatacagat ggctaacaga ccctctgctt ccagttcaaa tggtttaccg
    21481 tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg gcactaaagc acgttgttca
    21541 gggatcgaca ggcatccttc ttcaatgcct gtttcgccgc ttttttctaa aagctccgga
    21601 ttgattaaca ccagccgttc gtcacggttt tccgaaacat caataacaat gatacgttga
    21661 tggatatcga cctgggttgc cgccaggcca ataccttctt ctgcgtacat cgtctcgaac
    21721 atatcatcga cgatacgctg aatttctgca ttcacttctt ctaccggttt agcaactttg
    21781 cgaagccgct cgtccggaat atgtaacact tgcaaaactg acataaatct ccagagatgt
    21841 gttcaggagt tagaaagatt atttcttcta ttctagacaa atccccctct gattgacagc
    21901 atcactgacc aatcgcaaag attgctaagg ctgcttatgg cagggagata aggatggtcg
    21961 atacagaaat ttggctgcgt ttaatgagca tcagcagctt gtacggcgat gatatggtcc
    22021 gtatagctca ctggctggca aaacagtcgc atattgatgc ggttgtattg cagcaaactg
    22081 ggcttacatt gcggcaggca caacgctttc tttcatttcc gcgaaagagt atcgaaagct
    22141 cactttcttg gttggagcaa cccaaccatc atttaattcc tgcggacagc gaattttatc
    22201 ctcctcaact tcttgcgacg acagattacc ccggcgcact gtttgttgaa ggagaactgc
    22261 acgcgctgca ttcatttcag cttgccgtag tggggagtcg ggcgcattca tggtatggcg
    22321 agcgatgggg acgattattt tgcgaaactc tggcgacgcg tggagtgaca attacgagtg
    22381 gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc cttacaggta aatggcgtca
    22441 gcattgctgt attggggaat ggacttaata ccattcatcc gcgtcgccat gcccgactgg
    22501 ctaccagttt gcttgaacat ggtggggctc ttgtctcgga atttcccctc gatgttccac
    22561 cccttgctta caatttccca cgaagaaatc gcattatcag tggtctaagt aaaggtgtac
    22621 tggtggtgga agcggctttg cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc
    22681 aggggcgtga agtttttgcc ttgccgggtc cattagggaa tccgggaagc gaagggcctc
    22741 actggttaat aaaacaaggt gcgattcttg tgacggaacc ggaagaaatt ctggaaaact
    22801 tgcaatttgg attgcactgg ttgccagacg cccctgaaaa ttcattttat tcaccagatc
    22861 aggaagacgt ggcattgcca tttcctgagc tcctggctaa cgtaggagat gaggtaacac
    22921 ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc agaggtagta actcaactac
    22981 tcgaactgga gttagcagga tggatcgcag ctgtacccgg cggctatgtc cgattgagga
    23041 gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa cctatattca cacagaagct
    23101 gagttgcgtg tggatcaaga caaacttgaa caggatctta ccgacgcagg atttgatcga
    23161 gaagatatct acaatgccct gctttggctg gaaaagcttg cggattatca ggaaggactg
    23221 gcagaaccga tgcaactggc ctctgatcca ctctccatgc gtatttatac ccctgaagag
    23281 tgtgaaaggc tggatgccag ctgtcgtggt tttctgcttt tccttgagca aattcaggtg
    23341 cttaaccttg aaacccgtga aatggtgata gagcgagtgc tcgcgctgga taccgcagag
    23401 ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt tgttcaatat tccgggctgc
    23461 gaaaatgcgt accagcaaat ggaagaatta ctctttgaag tgaatgaagg tatgctgcat
    23521 taatcatttc tttaattcag cataagttgt tatggcgaaa tcagcactgt tcacggtgcg
    23581 taataatgag tcctgcccaa agtgcggggc tgaactggtt attcgatccg ggaaacacgg
    23641 tccgtttctt ggatgctcac agtatccggc gtgtgactac gtccgtcctc tgaaatcttc
    23701 agcggatgga catattgtca aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa
    23761 tctggtatta cgccagggac gctttggtat gtttattggt tgcagtaact accctgaatg
    23821 cgaacatacc gaacttatcg ataaaccgga cgaaacagca attacctgtc cccaatgtcg
    23881 gacgggccat ctggtccagc gccgttcccg ttatggcaaa acatttcact cctgtgatcg
    23941 ctacccggag tgtcaatttg ccattaactt caaacccata gctggagaat gccctgagtg
    24001 tcattatccg ctactcatcg aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag
    24061 taaacaatgt ggaaagccgg tttcggcgga ataataacgt gaataataac ctgcaaggag
    24121 acgctatcgc agctgcgata gatgtcctca atgaaaaacg tgtcatcgcc tatccaacgg
    24181 aagccgtttt cggtgtcggg tgcgatcctg atagcgaaac agcagtgatg cgactgttgg
    24241 agttaaaaca gcgtccggtt gataaggggc tgattttaat cgcggcaaat tacgagcagc
    24301 ttaaacccta tattgatgac accatgctga ctgatgcgca gcgtgaaacc attttttccc
    24361 gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc gacaacaccg cgctggttga
    24421 cgggccgctt tgattcgctt gctgtacgag tcaccgacca tccgttggtg gttgctttgt
    24481 gccaggctta tggtaaaccg ctggtttcta ccagtgccaa cttgagtgga ttgccacctt
    24541 gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg
    24601 aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc
    24661 gacaggggta acataatgga aacctatgct gtttttggta atccgatagc ccacagcaaa
    24721 tcgccattca ttcatcagca atttgctcag caactgaata ttgaacatcc ctatgggcgc
    24781 gtgttggcac ccatcaatga tttcatcaac acactgaacg ctttctttag tgctggtggt
    24841 aaaggtgcga atgtgacggt accttttaaa gaagaggctt ttgccagagc ggatgagctt
    24901 actgaacggg cagcgttggc tggtgctgtt aataccctca agcggttaga agatggacgc
    24961 ctgctgggtg acaataccga tggtataggt ttgttaagcg atctggaacg tctgtctttt
    25021 atccgtcctg gtttacgtat tctgctcatc ggcgctggtg gagcttctcg cggcgtacta
    25081 ctgccacttc tttccctgga ctgtgcggtg acaatcacta atcggacggt atcccgcgcg
    25141 gaagagttgg ctaaattgtt tgcgcacact ggcagtattc aggcgttggg tatggacgaa
    25201 ctggaaggtc atgagtttga tctcattatt aatgcaacat ccagtggcat cagtggtgat
    25261 attccggcga tcccgtcatc gcttattcat ccaggcattt attgctatga catgttctat
    25321 cagaaaggaa aaactccttt tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct
    25381 gatggtttag gaatgctggt ggcacaggcg gctcatgcct tccttctttg gcatggcgtt
    25441 ctgcctgatg tagaaccggt catcaaacta ttgcaacagg aattgtcagc gtgaatcagg
    25501 ccatccagtt tccggatagg gaagagtggg acgagaataa aagatgtgta tgttttcccg
    25561 ctctcgtgaa tggtatgcaa ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct
    25621 ttactggaga tacgccagaa cagtggttag cgagttttcg tcagcatcgc tgggacctgg
    25681 aagaagaagc ggaaaactta attcaggaac aaagtgaaga tgatcaaggt tgggtctggt
    25741 taccctgatc cagatattcg tccttccatt tcacgtaatt cttcgcggaa tagcgtaacc
    25801 cagccttctc ttcatcactt aacgggcgga tctgtttgac ggggctaccg agatacagat
    25861 atccgctctc cagccgttta ttttgtggga ccaggctacc cgcaccaatc atcacatcat
    25921 cttctactat tgcgccatca agtaaaattg agcccatccc aaccaaaact cgattgccaa
    25981 tggtgcagcc gtggagcatc accttgtgac caacagtgac atcttcgcca atggttaatg
    26041 ggttgccatc tgggttgtac gaggatttat gagtgacatg caacatactg ccatcctgga
    26101 tattggtgcg tgctccgatc tgtacataat gtacatctcc acgaatcaca acgagcggcc
    26161 agatccccac atcatcagcc agacgaacgt caccaatcac gacgctgcta tcgtcgatca
    26221 ttacgcgctg gccgatttgt ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac
    26281 tcacctcaac aataaatgat ttactaatga ctttgggtgc attattggcc ttgtgcaagt
    26341 cttttagcat gcaaaaaagc accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaagc
    26401 aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc
    26461 gcctccgttg agacgacaac gtgaaacact tcacaagatg gtcgtaacaa caaagagaaa
    26521 aaatcctgaa attcggggtt gactctgaaa gaggaaagcg taatatacgc cacctcgcga
    26581 cagtgagctg aaagccgcgt cgc
//
LOCUS       DAZKJR010000067        26421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKJR010000067 DAZKJR010000000
VERSION     DAZKJR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.067"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..1640
                     /gene="casA"
                     /locus_tag="ACHOWS_002771"
     CDS             132..1640
                     /gene="casA"
                     /locus_tag="ACHOWS_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFQ0048320.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LATLALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVCGIDLRSTVLLNVLTIPRLQKQFPNELHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANINFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
     gene            1633..2115
                     /gene="casB"
                     /locus_tag="ACHOWS_002772"
                     /gene_synonym="cse2"
     CDS             1633..2115
                     /gene="casB"
                     /locus_tag="ACHOWS_002772"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFQ0048321.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QSFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKTEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            2128..3219
                     /gene="cas7e"
                     /locus_tag="ACHOWS_002773"
     CDS             2128..3219
                     /gene="cas7e"
                     /locus_tag="ACHOWS_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFQ0048322.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KHAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKLVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITAQVKQMPTLEQLKSWVRNNGEA"
     gene            3222..3896
                     /gene="cas5e"
                     /locus_tag="ACHOWS_002774"
     CDS             3222..3896
                     /gene="cas5e"
                     /locus_tag="ACHOWS_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFQ0048323.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVAIWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTQPLFLG
                     TCQASDPQKALLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            3883..4482
                     /gene="cas6e"
                     /locus_tag="ACHOWS_002775"
     CDS             3883..4482
                     /gene="cas6e"
                     /locus_tag="ACHOWS_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFQ0048324.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            4498..5415
                     /gene="cas1e"
                     /locus_tag="ACHOWS_002776"
     CDS             4498..5415
                     /gene="cas1e"
                     /locus_tag="ACHOWS_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFQ0048325.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYVLLAKQYGVKW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRIPGEPDREVRLACRDIFRSSKTLVKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            5417..5701
                     /gene="cas2e"
                     /locus_tag="ACHOWS_002777"
     CDS             5417..5701
                     /gene="cas2e"
                     /locus_tag="ACHOWS_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFQ0048326.1"
                     /translation="MSMLVVVTENVPSRLRGRLAIWLLEVRAGVYVGDVSARVREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPV"
     repeat_region   5809..6201
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5809..5835
                     /rpt_unit_seq="gttccccgcgccagcggggataaaccg"
     gene            complement(6283..7320)
                     /gene="iap"
                     /locus_tag="ACHOWS_002778"
     CDS             complement(6283..7320)
                     /gene="iap"
                     /locus_tag="ACHOWS_002778"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFQ0048327.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            7572..8480
                     /gene="cysD"
                     /locus_tag="ACHOWS_002779"
     CDS             7572..8480
                     /gene="cysD"
                     /locus_tag="ACHOWS_002779"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFQ0048328.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            8482..9909
                     /gene="cysN"
                     /locus_tag="ACHOWS_002780"
     CDS             8482..9909
                     /gene="cysN"
                     /locus_tag="ACHOWS_002780"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFQ0048329.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            9909..10514
                     /gene="cysC"
                     /locus_tag="ACHOWS_002781"
     CDS             9909..10514
                     /gene="cysC"
                     /locus_tag="ACHOWS_002781"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFQ0048330.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            10564..10887
                     /locus_tag="ACHOWS_002782"
     CDS             10564..10887
                     /locus_tag="ACHOWS_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFQ0048331.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            11080..11391
                     /gene="ftsB"
                     /locus_tag="ACHOWS_002783"
     CDS             11080..11391
                     /gene="ftsB"
                     /locus_tag="ACHOWS_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFQ0048332.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            11410..12120
                     /gene="ispD"
                     /locus_tag="ACHOWS_002784"
     CDS             11410..12120
                     /gene="ispD"
                     /locus_tag="ACHOWS_002784"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFQ0048333.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            12120..12599
                     /gene="ispF"
                     /locus_tag="ACHOWS_002785"
     CDS             12120..12599
                     /gene="ispF"
                     /locus_tag="ACHOWS_002785"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFQ0048334.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            12596..13645
                     /gene="truD"
                     /locus_tag="ACHOWS_002786"
     CDS             12596..13645
                     /gene="truD"
                     /locus_tag="ACHOWS_002786"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFQ0048335.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQKLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            13626..14387
                     /gene="surE"
                     /locus_tag="ACHOWS_002787"
     CDS             13626..14387
                     /gene="surE"
                     /locus_tag="ACHOWS_002787"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFQ0048336.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            14381..15007
                     /gene="pcm"
                     /locus_tag="ACHOWS_002788"
     CDS             14381..15007
                     /gene="pcm"
                     /locus_tag="ACHOWS_002788"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFQ0048337.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            15192..16286
                     /gene="nlpD"
                     /locus_tag="ACHOWS_002789"
     CDS             15192..16286
                     /gene="nlpD"
                     /locus_tag="ACHOWS_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFQ0048338.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            16349..17341
                     /gene="rpoS"
                     /locus_tag="ACHOWS_002790"
     CDS             16349..17341
                     /gene="rpoS"
                     /locus_tag="ACHOWS_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFQ0048339.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(17462..17869)
                     /locus_tag="ACHOWS_002791"
     CDS             complement(17462..17869)
                     /locus_tag="ACHOWS_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0048340.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMSK"
     gene            18016..18609
                     /locus_tag="ACHOWS_002792"
     CDS             18016..18609
                     /locus_tag="ACHOWS_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFQ0048341.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            18609..20036
                     /locus_tag="ACHOWS_002793"
     CDS             18609..20036
                     /locus_tag="ACHOWS_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFQ0048342.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFNILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            20047..20283
                     /locus_tag="ACHOWS_002794"
     CDS             20047..20283
                     /locus_tag="ACHOWS_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFQ0048343.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWMVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(20321..21685)
                     /locus_tag="ACHOWS_002795"
     CDS             complement(20321..21685)
                     /locus_tag="ACHOWS_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFQ0048344.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCI
                     WAVI"
     gene            complement(21774..22550)
                     /locus_tag="ACHOWS_002796"
     CDS             complement(21774..22550)
                     /locus_tag="ACHOWS_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFQ0048345.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLAQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADHFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(22555..23193)
                     /locus_tag="ACHOWS_002797"
     CDS             complement(22555..23193)
                     /locus_tag="ACHOWS_002797"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFQ0048346.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(23190..24452)
                     /gene="otnK"
                     /locus_tag="ACHOWS_002798"
     CDS             complement(23190..24452)
                     /gene="otnK"
                     /locus_tag="ACHOWS_002798"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFQ0048347.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(24449..25357)
                     /gene="ltnD"
                     /locus_tag="ACHOWS_002799"
     CDS             complement(24449..25357)
                     /gene="ltnD"
                     /locus_tag="ACHOWS_002799"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFQ0048348.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            25553..26320
                     /gene="ygbI"
                     /locus_tag="ACHOWS_002800"
     CDS             25553..26320
                     /gene="ygbI"
                     /locus_tag="ACHOWS_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFQ0048349.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
BASE COUNT         6539 a         6487 c         7027 g         6368 t
ORIGIN      
        1 gaatatattt ttgcagtgaa tgtgattatt gaattaatta cgccgtattt tttctttgtt
       61 tttaccgata acggaagtgt gccgacgcat agaaatgcag gagaaatgtc ggagcatatg
      121 aaggagaaca aatgaatttg cttattgata actggatccc tgtacgcccg cgaaacgggg
      181 ggaaagtcca aatcataaat ctgcaatcgc tatactgcag tagagatcag tggcgattaa
      241 gtttgccccg tgacgatatg gaactggcca ctttagcact gctggtttgc attgggcaaa
      301 ttatcgcccc ggcaaaagat gacgttgaat ttcgacatcg cataatgaat ccgctcactg
      361 aagatgagtt tcaacaactc atcgcgccgt ggatagatat gttctacctt aatcacgcag
      421 aacatccctt tatgcagacc aaaggtgtca aagcaaatga tgtgactcca atggaaaaac
      481 tgttggctgg ggtaagcggc gcgacgaatt gtgcatttgt caatcaaccg gggcagggtg
      541 aagcattatg tggtggatgc actgcgattg cgttattcaa ccaggcgaat caggcacctg
      601 gttttggtgg tggctttaaa agcggtttac gtggaggaac acctataacc accttcgtat
      661 gtgggatcga tcttcgctcg acggtattac tcaatgtcct cacaatacct cgtcttcaaa
      721 aacaatttcc caatgaatta catacggaaa accaacctac ctgggttaag cctgtcaagc
      781 cgaatgaatc ggtgcctgct tcttcaattg ggtttgtccg tggtttattc tggcaaccag
      841 cgcatattga actatgcgat cccattggga ttggtaaatg ttcttgctgt ggtcaggaaa
      901 gcaatttgcg ttataccggt tttcttaagg aaaaatttac ctttacagtt aatgggctat
      961 ggccccatcc gcattcccct tgtctggtaa cagtcaagaa aggggaggtt gaggaaaaat
     1021 ttcttgcttt caccacctcc gcaccatcat ggacacaaat cagccgagtt gtggtagata
     1081 agattattca aaatgaaaat ggaaatcgcg tggcggcggt tgtgaatcaa ttcagaaata
     1141 ttgcgccgca aagtcctctt gaattgatta tggggggata tcgtaataat caagcatcta
     1201 ttcttgaacg gcgtcatgat gtgttgatgt ttaatcaggg gtggcaacaa tacggcaatg
     1261 tgataaacga aatagtgact gttggtttgg gatataaaac agccttacgc aaggcgttat
     1321 atacctttgc agaagggttt aaaaataaag acttcaaagg ggccggagtt tctgttcatg
     1381 agactgcaga aaggcatttc tatcgacaga gtgaattatt aattcccgat gtattggcga
     1441 atattaattt ttcccaagct gatgaggtaa tagctgattt acgagacaaa cttcatcaat
     1501 tgtgtgaaat gctatttaat caatctgtag ctccctatgc acatcatcct aaattaataa
     1561 gcacattagc gcttgcccgc gccacgctat acaaacattt acgggagtta aaacctcaag
     1621 gagggccatc aaatggctga tgaaattgat gcaatggctt tatatcgagc ctggcaacaa
     1681 ctggataatg gagcatgtgc gcaaattaga cgtgtttcag aacctgatga attacgcgat
     1741 atccctgcgt tttataggct ggtgcaatct tttggttggg aaaacccacg tcaccagcag
     1801 gctcttttgc gcatggtgtt ttgcctgagc gcaggaaaga atgtcatccg acatcaggac
     1861 aaaaaaacgg agcaaacaac aggtatctcg ttgggaagag ctttagccaa tagtggaaga
     1921 attaacgagc gccgtatctt tcaattaatt cgggctgaca gaacagccga tatggtccag
     1981 ttacgtcgat tacttactca cgccgaaccc gtacttgact ggccattaat ggccaggatg
     2041 ttgacctggt ggggaaagcg cgaacgccag caacttctgg aagattttgt attgaccaca
     2101 aacaaaaatg cgtaaggaaa cctttctatg tctaacttta tcaatattca tgttctgatc
     2161 tctcacagcc cttcatgtct gaaccgcgac gatatgaaca tgcagaaaga cgctattttc
     2221 ggcgggaaaa gacgagtaag aatttcaagt caaagcctta aacatgcgat gcgtaaaagt
     2281 gattattacg cacaaaatat tggtgaatcc agtctcagaa caattcatct tgcacaatta
     2341 cgtgatgttc ttcggcaaaa acttggtgaa cgttttgacc aaaaaatcat cgataagaca
     2401 ttagcgctgc tctccggtaa attagttgat gaagccgaaa agatttctgc cgatgcggtt
     2461 actccctggg ttgtgggaga aatagcctgg ttctgtgagc aggttgcaaa agcagaggct
     2521 gataatctgg atgataaaaa gctgctcaaa gttcttaagg aagatattgc cgccatacgt
     2581 gtgaatttac agcagggtgt tgatattgcg cttagtggaa gaatggcaac cagcggcatg
     2641 atgagtgagt tgggaaaagt tgatggtgca atgtccattg cgcatgcgat cactactcat
     2701 caggttgatt ctgatattga ctggttcacc gctgtagatg atttacagga acaaggttct
     2761 gcacatctgg gaactcagga attttcatcg ggtgtttttt atcgttatgc caacattaac
     2821 ctcgctcaac ttcaggaaaa tttaggtggt gcctccaggg agcaggctct ggaaattgca
     2881 acccatgttg ttcatatgct ggcaacagag gtccctggag caaaacagcg tacttatgcc
     2941 gcttttaacc ctgcggatat ggtaatggtt aatttctccg atatgccact ttctatggca
     3001 aatgcttttg aaaaagcggt taaagcgaaa gatggctttt tgcaaccgtc tatacaggcg
     3061 tttaatcaat attgggatcg cgttgccaat ggatatggtc tgaacggagc tgctgcgcaa
     3121 ttcagcttat ctgatgtaga cccaattact gctcaagtta aacaaatgcc tactttagaa
     3181 cagttaaaat cctgggttcg taataatggc gaggcgtgaa catgagatct tatttgatct
     3241 tgcggcttgc tgggccaatg caagcctggg ggcagccgac ctttgaagga acgcgaccta
     3301 ccggaagatt tccgacccga agcgggttat tagggctact cggggcttgt cttgggatcc
     3361 aacgtgatga tacttcttca ttacaggcgt tatcagagag tgtgcaattt gcagtgcgct
     3421 gcgatgaact cattcttgac gatcgtcgtg tgtctgtaac ggggttgcgt gattaccata
     3481 cagtccttgg agcgcgagaa gattaccgtg gtttgaaaag tcatgaaacg attcaaacat
     3541 ggcgcgaata tttatgtgat gcctccttta ccgtcgctat ctggttaaca ccacaggcaa
     3601 cgatggttat gtcagaactt gaaaaagcag tattaaagcc tcggtataca ccgtatctgg
     3661 ggcggaggag ttgtccgcta acacaaccgc tttttctcgg gacatgtcag gcgtcggatc
     3721 ctcagaaggc gctattaaat tatgagcccg ttggcggcga tatttatagt gaggaatcag
     3781 ttgacgggca tcatttaaaa tttacggtgc gcgacgaacc gatgatcacc ttacctcgac
     3841 aatttgcttc ccgagaatgg tatgtgatta aaggaggtat ggatgtatct cagtaaagtc
     3901 atcattgcca gggcctggag cagggatctt taccaacttc accagggatt atggcattta
     3961 tttccaaaca gaccggatgc tgctcgtgat tttctttttc atgttgagaa gcgaaacaca
     4021 ccagaaggct gtcatgtttt attgcagtca gcgcaaatgc ctgtttctac tgccgttgcg
     4081 acagtcatta aaactaaaca ggttgaattt caacttcagg ttggtgttcc actctatttt
     4141 cggcttcggg caaatccgat caaaactatt ctcgacaatc aaaagcgcct ggacagtaaa
     4201 gggaatatta aacgctgtcg ggttccgtta ataaaagaag cagaacaaat cgcgtggttg
     4261 caacgtaaat tgggcaatgc ggcgcgcgtt gaagatgtgc atcccatatc ggaacggcca
     4321 cagtattttt ctggtgatgg taaaagtgga aagatccaaa cggtttgctt tgaaggggtg
     4381 atcaccatca acgacgcgcc agcgttaata gatcttgtac agcaaggtat tgggccagct
     4441 aaatcgatgg gatgtggctt gctatctttg gctccactgt gattgaggtg taataaaatg
     4501 acctggcttc cccttaatcc cattccactc aaagatcgcg tctccatgat ctttctgcaa
     4561 tatgggcaga tcgatgtaat agatggcgcg tttgtactta tcgacaagac agggatccgc
     4621 actcatattc ctgttggctc ggttgcctgc atcatgttgg aacctggtac acgggtttcg
     4681 catgcagctg tacgcctggc tgcgcaagtt ggaacattat tggtatgggt gggggaagcg
     4741 ggcgttcgtg tttatgcttc tggtcagcct ggtggtgcgc gttcagataa gctgctctat
     4801 caggcaaaac ttgctctgga tgaagatttg cgtctgaagg tcgtacgtaa aatgtttgaa
     4861 cttcggtttg gagaacctgc gcctgcccgg cgctccgtag agcaactcag aggtatagaa
     4921 ggaagtcgtg tgcgagcaac ctatgtactt ctggcgaagc aatacggggt gaaatggaat
     4981 ggacgtcgtt acgatccgaa agactgggaa aagggcgata cggtcaacca atgcattagc
     5041 gctgcaactt cttgtttata tggcgtaact gaagcggcga tacttgcagc tggttatgca
     5101 ccagctattg ggtttgtgca tacaggaaag cctctttcct ttgtttacga tatcgcggac
     5161 atcattaaat ttgacactgt tgtaccgaaa gcttttgaga tagcgcgtcg tattcctggt
     5221 gagccggacc gggaagtccg tttggcgtgc agggatattt ttcgcagtag taaaacatta
     5281 gtcaaattga ttccgcttat agaggacgtg cttgctgctg gggaaataca gccgccggcc
     5341 ccacctgaag atgcgcagcc tgttgctatt ccacttcccg tttctctggg agatgccgga
     5401 catcggagta gctgagatga gtatgttggt cgtggtcact gaaaatgtac cttcgcgctt
     5461 acgaggcaga ttagccatct ggttgttgga ggtacgtgca ggggtgtatg taggtgatgt
     5521 atccgcaaga gtccgtgaaa tgatctggga acaaatatct ggactggcgg aagaaggcaa
     5581 tgtggtgatg gcatgggcta cgaatacgga atcgggattt gagttccaga catttggggt
     5641 aaacaggcgt actccggtag atttggatgg tttaaggttg gtctcttttt tacctgttta
     5701 aaaacaaaga attagctgat ctttaataat aaggaaatgt tacattaagg ttggtgggtt
     5761 gtttttatgg gaaaaaatgc tttaagaaca aatgtatact tttagagagt tccccgcgcc
     5821 agcggggata aaccggtgcc gggttaatgg gggaggctgg ccccgaggag ttccccgcgc
     5881 cagcggggat aaaccgtaaa taacggcatt accgagtgtg aaactgaaga gttccccgcg
     5941 ccagcgggga taaaccgatt ccgtcaaatt tatcactcaa cgccagttcg tgttccccgc
     6001 gccagcgggg ataaaccgag attggttttc cagtctttgg cggttggcaa gtgttccccg
     6061 cgccagcggg gataaaccgc tgtgccggta gccccctccc tgttcagcct cgtgttcccc
     6121 gcgccagcgg ggataaaccg ggtaaaaaca cggtctgaac cgacattcat gtgtgttccc
     6181 cgcgtcagcg gggataaacc gggcgcactg gatgcgatga tggatatcac ttagaattcc
     6241 ccgcccctgc ggtagaactc ccaactccca ttttcaaacc catcaagacg ccttcgccaa
     6301 ctccttcacc agaggtagca ttatccgcat aacatcacgg cagcgacgtt ctattcttcc
     6361 aggaagcgcc ttatcaatat gttgttgatt atccagtctt acgtcatgcc agctatttcc
     6421 cgcagggaat gcggctgttt ttgcgcgttg ctgataacca tccttattcc caagattcca
     6481 gttagtcgct tccaccgaaa gtacagcaat accggctttg tcgaatattt ctgcgtcatt
     6541 acaacaccca gtgcctttcg gataattttt attcaaaccc ggattggtcg ttgcggcaat
     6601 tccatgacta cgcgcaattg ccagcgccct gtcgcgcgtt aatttcctta ctgcttcagg
     6661 ggtttttaca ccactattga aatacaattt atcgccaaca attaagttat cgagattaat
     6721 caccagcagc gtatttttct tttcggtgtc actcatccgc ttgagtaaat tctcagcacc
     6781 taatttccct tcctcttcac cactggtcgc cacaaatcga ataccatact cggtaggcgt
     6841 atttttcagg cgttctgcca gttccagcat gacacctaaa cctgcggcgt tatcatctat
     6901 tccttgtaac gtcagcccgc cgagattggc atcggcgtca gcatcgctca gcggagcgta
     6961 agtatccaga tgcgccataa tgatgatctg ctgcggagct ttgccttcat gagcggcaat
     7021 caccgtactt cccgtcacgt tatgccagct cttgcgatta tcgcgggcgg tataaatata
     7081 ccgactatta aatgtccgaa tatcactgcg ataacccatt tgctgaaact gttggcgaat
     7141 ataatcggca gataacattt ctgcaggagt tccggtcatg cgtcccggaa agaaagtagc
     7201 aatatgtcgt gcctgggtat tagcaaaatc gccaggttta ggtgacgagg cgtgtacggg
     7261 gaggataaag catacgccga gcgccagggc agcggtacgg tggcgcaatg cggaaaacat
     7321 agtgagtcct taaataccat gcaaattttt ttaccgtcat agtatgaaac tgccgctgcg
     7381 ctaaaacaat ttcaaatctt cctaaacgcc cgaaatccgg tgccttaagc actttttgat
     7441 attagctttg ccaaatcgtt attccgttaa ggaactactc attctaattg gtaatttcat
     7501 tcgttctctt acgctcccta tagtcgaaac atctgatggc aagaaaatag cggtattgca
     7561 aaggaacggt tatggatcaa aaacgactta cccacctgcg gcaactggag gcggaaagca
     7621 tccacattat tcgcgaggtg gctgcagaat tctcaaatcc ggtgatgctc tactctatcg
     7681 gtaaagattc cagcgtcatg ctgcatctgg cgcgcaaggc gttttatcca ggtacgctgc
     7741 ctttcccgtt gctgcatgtc gataccggct ggaaattccg cgagatgtat gagttccgcg
     7801 atcgtacggc gaaagcctac ggctgcgagc tgctggtgca taaaaacccg gaaggcgtgg
     7861 cgatggggat taatccattc gtacacggca gcgcgaaaca taccgatatt atgaaaactg
     7921 aaggcctgaa acaggcgctg aacaaatacg gttttgatgc cgccttcggt ggtgcgcgcc
     7981 gtgacgaaga gaaatcccgc gctaaagagc gaatctactc tttccgtgac cgcttccatc
     8041 gctgggatcc gaaaaatcag cgcccggagc tgtggcacaa ctacaacggg caaattaaca
     8101 aaggcgaaag catccgcgtc ttcccgctct ctaactggac cgagcaggat atctggcaat
     8161 acatctggct ggaaaatatc gacattgttc cgctgtatct tgctgcggaa cgtccggttc
     8221 tggaacgcga cggtatgttg atgatgattg atgacaaccg tatcgacctg caaccgggcg
     8281 aagtgattaa aaaacggatg gtgcgtttcc gcacactggg ctgttggccg ctgaccggcg
     8341 cggtggagtc aaatgcgcaa acactgccgg agatcatcga agagatgctg gtttccacca
     8401 ccagtgaacg tcagggccgc gtaattgacc gcgaccaggc ggggtctatg gagctgaaaa
     8461 aacgtcaggg gtatttttaa gatgaacacc gcacttgcac aacaaatcgc caatgaaggc
     8521 ggcgtcgaag catggatgat tgcgcaacaa cataaaagcc tgctgcgttt tctgacctgt
     8581 ggtagcgtcg atgacggcaa aagtaccctg attggtcgcc tgctgcacga tacccgccaa
     8641 atctacgaag atcagctctc atcgctgcat aacgacagta agcgtcacgg cacccagggc
     8701 gaaaagctgg atctggcact gctggtggac ggcctgcaag ctgagcgcga acagggcatc
     8761 actattgatg tggcctaccg ctatttctct accgagaagc gtaaatttat tatcgccgac
     8821 accccagggc acgagcagta cacccgcaat atggcgactg gcgcatcgac atgtgaactg
     8881 gcgatcttac tgatcgatgc ccgtaaaggc gtgctcgatc aaacccgtcg tcatagtttt
     8941 atctccacac tgttggggat caaacatctg gtcgtggcga tcaacaaaat ggatctggtg
     9001 gattacagtg aagagacgtt cacccgtatt cgtgaagatt atctgacttt tgccgggcag
     9061 ctgccgggta atctggatat ccgctttgtg ccgctctccg cactggaagg cgacaacgtg
     9121 gcttcgcaaa gtgaaagtat gccgtggtac agcggtccga cactgctaga agtgctggaa
     9181 accgtggaga tccagcgagt ggtggatgct cagccaatgc gcttcccggt gcagtacgtt
     9241 aaccgtccga atctcgattt tcgtggctac gccggaacgc tggcatccgg tcgcgtggaa
     9301 gtcgggcaac gtgtcaaagt gctgccctct ggtgtggaat caaacgtcgc gcggatcgtg
     9361 acttttgatg gcgatcgcga agaagccttt gccggagaag cgatcaccct ggtgctgacg
     9421 gatgagatcg acatcagccg tggcgatctg ctgctggcgg cagacgaagc gttaccggct
     9481 gtgcagagcg cgtcggtgga tgtggtatgg atggcggaac agccgctttc cccgggccag
     9541 agttatgaca tcaaaattgc cggtaagaag acgcgtgctc gtgttgatgg cattcgttat
     9601 caggttgata tcaataacct tacccaacgc gaagttgaaa acctgccgct gaacggcatc
     9661 ggcctggtag atctcacctt tgacgagcca ctggtgttag atcgttatca gcaaaacccg
     9721 gttaccggtg ggctgatttt tatcgatcgc ctgagtaatg tgaccgtagg tgccggtatg
     9781 gtgcacgaac cagttagcca ggcaactgct gcgccatctg aattcagtgc attcgaactg
     9841 gaattgaatg ccctggttcg ccgccatttc ccgcattggg gtgcgcgcga tttactggga
     9901 gataaataat ggcgctgcat gacgaaaacg tcgtctggca tagccaccca gtaactccgc
     9961 aacaacgcga acagcaccac ggtcatcgtg gtgttgtgct gtggtttacc ggcctctctg
    10021 gttccggtaa atcaaccgtc gcgggggcgc tggaggaggc gttacataaa cttggcgtca
    10081 gtacgtatct gctggatggc gacaacgttc gccacggatt atgcagcgat ctcggtttta
    10141 gcgatgccga ccgtaaagag aatatccgtc gggttggtga agtggcgaat ttgatggttg
    10201 aagccggact ggtggtgctg accgcattta tctcgccaca ccgcgccgaa cgccagatgg
    10261 ttcgcgaacg cgtaggagaa gggcgcttta tcgaagtgtt tgtcgatacg ccgctggcga
    10321 tttgcgaagc ccgcgatcca aaaggtttat ataagaaagc gcgtgccggt gaactgcgca
    10381 actttacggg aatagattcc gtttacgaag cgcctgaatc ggcagaaatt catctcaatg
    10441 gtgaacaatt agtaacaaat ttggtacagc aattattaga tctgttgaga cagaacgata
    10501 ttatcagatc ctgagacacc gccgggtttc catgcccggt tcgtcaaagt aacaggatca
    10561 gatatgcgta acagccacaa cattacatta acaaataacg acagccttac tgaggttgaa
    10621 gacaccacat ggtcgctgcc aggcgctgtg gttggttttt tatcgtggct gtgcgcgttg
    10681 gcaattccga tgttgattta tggctctaac acgctgctct tttttatcta cacctggcct
    10741 ttctttctgg cgctgatgcc cgtcgctgta gtggtgggga ttgcactgca ttcattgatg
    10801 gacggaaagc tacgctacag tattgttttc actctggcga cggttggcat tatgtttggt
    10861 gcgctgttta tgtggctact gggttaattg ttctttcctc tctattctgt tcataattca
    10921 atccgtaact aataatgaga ttatgttctg cacgccctgg gtatacgtaa caatggacaa
    10981 atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca gaggatgtag agtcgtcttc
    11041 ggatgcatgg gatgaagatg ccgtttttca gggggcagga tgggtaaact aacgctgctg
    11101 ttgctggcta ttctggtctg gctacagtat tcgctgtggt tcggtaagaa cggtatacat
    11161 gactatactc gcgtcaataa tgatgtggcg gcacagcaag ctacaaacgc gaaacttaaa
    11221 gcgcgaaacg atcagctttt tgccgaaatt gacgatctca atggcggcca ggaggcgctc
    11281 gaagagcgtg cgcgtaatga actcagcatg accaggccgg gcgaaacgtt ttatcgtctg
    11341 gtgcctgacg cgtcaaagcg cgcacagtct gcggggcaaa ataatcgata aatcagcccg
    11401 ggaattaaca tggcaaccac tcatttggat gtttgcgccg tggttccggc ggccggattt
    11461 ggccgccgaa tgcaaacgga atgtcctaag caatatctct caatcggtaa tcaaaccatt
    11521 cttgaacact cggtgtatgc gctgctggcg catcccaggg tgaaacgtgt cgtcattgcc
    11581 ataagtcctg gcgatagccg ctttgcacaa cttcctctgg cgaatcatcc acgaatcacg
    11641 gttgttgatg gtggtgaaga gcgtgccgat tccgtgctgg caggtctgaa agccgctggc
    11701 gacgcgcagt gggtattggt gcatgacgcc gctcgtcctt gtctgcatca ggatgacctc
    11761 gcgcgattgt tggcgttgag cgaaaccagc cgcacggggg ggatcctcgc tgcgccggtg
    11821 cgcgatacga tgaaacgtgc cgaaccgggc aaaaatgcca ttgctcatac cgttgatcgc
    11881 aacggcttat ggcacgcgct gacgccgcaa tttttccctc gtgagctgtt acatgactgc
    11941 ctgacgcgcg ctctaaatga aggcgctacc attaccgatg aggcctcggc gctggaatat
    12001 tgcggttttc atccgcaact tgttgaaggc cgcgcggata acatcaaggt aacgcgcccg
    12061 gaggatttgg cgctggccga attttacctc acccgaacca tccatcagga gaatacataa
    12121 tgcgaattgg acacggtttt gacgtacacg cctttggcgg tgaaggccca attatcattg
    12181 gtggcgtacg cattccttac gaaaaagggc tgctggcgca ttctgatggc gacgtggcgc
    12241 tccatgcgtt gaccgatgca ttgcttggcg cggcggcgct gggagatata ggcaagctgt
    12301 tcccggatac cgatccggca tttaaaggtg ccgacagccg cgagctgttg cgcgaagcct
    12361 ggcgtcgtat tcaggcgaaa ggttatgccc tcggcaacgt cgatgtcact atcatcgctc
    12421 aggcaccgaa gatgttgccg cacattccac aaatgcgcgt gtttattgcc gaagatctcg
    12481 gctgccatat ggatgatgtt aacgtgaaag ccactactac ggaaaaactg ggatttaccg
    12541 gacgtgggga agggattgcc tgtgaagcgg tggcgctact cattaaggca acaaaatgat
    12601 tgagtttgat aatctcactt acctccacgg taaaccgcaa ggcaccgggc tgctgaaagc
    12661 caatccggaa gactttgtgg tggtggaaga tttgggcttt gagcctgatg gtgaaggtga
    12721 gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc cgttttgtgg cggatgcact
    12781 ggcgaaattc ctgaaaattc atgcccgtga agtcagcttc gctgggcaaa aagataaaca
    12841 tgctgttacg gaacagtggt tatgcgctcg cgtgccgggc aaagagatgc ccgatctgag
    12901 cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg cggcacaagc gcaagctgcg
    12961 tttaggggcg ctgaaaggta acgcctttac cctggtacta cgcgaagtga gcaatcgcga
    13021 tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt gtaccgaact acttcggtgc
    13081 ccaacgtttt gggattggcg gtagcaactt gcagggcgcg ctgcgttggg cgcaaaccaa
    13141 tactccggtg cgcgatcgca ataaacggag tttttggttg tcggcagccc gcagtgcgtt
    13201 gtttaatcag attgttgctg agcgcctcaa aaaagcagac gttaatcaag ttgttgacgg
    13261 cgatgcgcta caattagccg gacgtggtag ctggtttgtt gcaaccaccg aagaactggc
    13321 ggaattacag cgtcgcgtca acgataaaga gctgatgata accgccgcat tgccgggcag
    13381 tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa caagcagctg tcgccgaaga
    13441 aactgaatta caaactttac tggtgcgtga aaaagttgaa gccgcgcgca gagcgatgct
    13501 gctgtatccg caaaaattaa gctggaactg gtgggatgac gtcaccgtag aaatccgttt
    13561 ctggcttccg gcgggaagtt ttgcaaccag cgttgtcagg gaacttatca acacaacagg
    13621 tgattatgcg catattgctg agtaatgatg acggggtaca tgcacccggt atacaaacgc
    13681 tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt cgcccccgat cgtaaccgca
    13741 gcggcgcttc aaattctctg acactggaat cctccctgcg cacgtttacc tttgaaaatg
    13801 gtgatattgc tgtgcaaatg ggaaccccga ccgattgcgt ctatcttggc gtgaatgctc
    13861 tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa cgccgggccg aatctggggg
    13921 atgatgttat ttattccggt acggtagccg ccgcgatgga aggccgtcat ttaggttttc
    13981 cggcgctggc cgtctcgctt gacgggcata aacattacga cactgccgcg gcggtaacct
    14041 gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac cggacgtatt cttaatatta
    14101 acgttccgga tttaccattg gatcaaatca aaggtattcg cgtgacgcgc tgcggtacac
    14161 gacatccggc agatcaggtg atcccgcagc aagatccgcg cggcaatacg ctgtactgga
    14221 ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac cgattttgct gcggtagatg
    14281 agggctatgt ttccatcacg ccgctgcatg tggatttaac tgcgcatagc gcgcaagatg
    14341 tggtttcaga ctggttaaac agcgtgggag ttggcacgca atggtaagca gacgcgtaca
    14401 agcacttctg gatcaattac gtgcgcaagg tattcaggat gagctggtgc tgaatgcact
    14461 tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt gaacaaaaag cctgggacaa
    14521 tatcgccttg ccgataggtc aggggcagac aatttcgcag ccgtatatgg tggcgcgaat
    14581 gaccgaatta ctcgaactga cgccgcagtc gcgggtgctg gaaattggca ccggttcggg
    14641 atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt tgctcggttg aacggattaa
    14701 aggcttgcag tggcaggcgc gtcgccgcct gaaaaatctt gatttacata atgtttcaac
    14761 ccgtcatggc gatggatggc aaggttggca ggcacgtgcg ccgtttgacg ctatcattgt
    14821 tacggcggca ccgccggaaa ttccaactgc gctaatgacg cagctggacg aaggcggaat
    14881 tctcgtctta cccgtagggg aggagcacca gtatttgaaa cgggtgcgtc gtcggggagg
    14941 cgaatttatt atcgataccg tggaggccgt gcgctttgtc cctttagtga agggtgagct
    15001 ggcttaaaac gtgaggaaat acctggattt ttcctggtta ttttgccgca ggtcagcgta
    15061 tcgtgaacat cttttccagt gttcagtatg gtgccttgca cggtaattat gtcactggtt
    15121 attaaccaat ttttcctggg ggataaatga gcgcgggaag cccaaaattc accgttcgcc
    15181 gcattgcggc tttgtcactg gtttcgctat ggctggcagg ctgttctgac acttcaaatc
    15241 caccggcacc ggtcagctcc gttaatggca atgcgcctgc aaataccaat tctggtatgt
    15301 tgattacgcc gccgccgaaa atggggacga cgtctacagc gcagcaaccg caaattcagc
    15361 cggtacagca gccacaaatt caggctactc aacaaccgca aatccagcca gtacagccag
    15421 tagctcagca gccggtacag atggaaaacg gacgcatcgt ctacaaccgt cagtatggga
    15481 acattccgaa aggcagttat agcggcagta cctataccgt gaaaaaaggc gacacacttt
    15541 tctatatcgc ctggattact ggcaacgatt tccgtgacct tgctcagcgc aacaatattc
    15601 aggcaccata cgcgctgaac gtcggtcaga ccttacaggt gggtaatgct tccggtacgc
    15661 caatcactgg cggaaatgcc attacccagg ccgacgcagc agagcaagga gttgtgatca
    15721 agcctgcaca aaattccacc gttgctgttg cgtcgcaacc gacaattacg tattctgagt
    15781 cttcgggtga acagagtgct aacaaaatgt tgccgaacaa caagccaact gcgaccacgg
    15841 tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga gccgactgtc agcagtacat
    15901 caaccagtac gcctatctcc acctggcgct ggccgactga gggcaaagtg atcgaaacct
    15961 ttggcgcttc tgaggggggc aacaagggga ttgatatcgc aggcagcaaa ggacaggcaa
    16021 ttatcgcgac cgcagatggc cgcgttgttt atgccggtaa cgcgctgcgc ggctacggta
    16081 atctgattat catcaaacat aatgatgatt acctgagtgc ctacgcccat aacgacacaa
    16141 tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa aatagcaacc atgggtagca
    16201 ccggaaccag ttcaacacgc ttgcattttg aaattcgtta caaggggaaa tccgtaaacc
    16261 cgctgcgtta tttgccgcag cgataaatcg gcggaaccag gcttttgctt gaatgttccg
    16321 tcaagggatc acgggtagga gccaccttat gagtcagaat acgctgaaag ttcatgattt
    16381 aaatgaagat gcggaatttg atgagaacgg agttgaggtt tttgacgaaa aggccttagt
    16441 agaagaggaa cccagtgata acgatttggc cgaagaggaa ctgttatcgc agggagccac
    16501 acagcgtgtg ctggacgcga ctcagcttta ccttggtgag attggttatt caccactgtt
    16561 aacggccgaa gaagaagttt attttgcgcg tcgcgcactg cgtggagatg ttgcctcccg
    16621 ccgccggatg atcgagagta acttgcgtct ggtggtaaaa attgcccgcc gttatggcaa
    16681 tcgtggtctg gcgttgctgg acctgatcga agagggcaac ctggggctga tccgcgcggt
    16741 agagaagttt gacccggaac gtggtttccg cttctcaaca tacgcaacct ggtggattcg
    16801 ccagacgatc gaacgggcga ttatgaacca aacccgtact attcgtttgc ctattcacat
    16861 cgtaaaggag ctgaacgttt acctgcgaac cgcacgcgag ttgtcccata aactggacca
    16921 cgaaccaagt gcggaagaga tcgcagagca actggataag ccagttgatg acgtcagccg
    16981 tatgcttcgt cttaacgagc gcattacctc ggtagacacc ccgctgggtg gtgattccga
    17041 aaaagcgttg ctggacatcc tggccgatga aaaagagaac ggtccggaag ataccacgca
    17101 agatgacgat atgaagcaga gcatcgtcaa atggctgttc gagctgaacg ccaaacagcg
    17161 tgaagtactg gcacgtcgat tcggtttgct ggggtacgaa gcggcaacac tggaagatgt
    17221 aggtcgtgaa attggcctca cccgtgaacg tgttcgccag attcaggttg aaggcctgcg
    17281 ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc gaagcgctgt tccgcgaata
    17341 agtaagcatc tgtcagaaag gccagtctca agcgaggctg gcctttttct tttgtttggg
    17401 aagtcggtgt gttttgtcgc ctggataaga ctcgtcagcg tcctacccgg caatcagcat
    17461 cctatttgct catcatcttg cgcaccaact gcataaacag ctcctgctct tctgcactca
    17521 aacgccccaa aaattcctca tccacactgt caccaatggg gatcgccgca gcaagtacct
    17581 tttctccttc ggcagtcagc cagacaaagc gtcgccgctt gtcagcggcg tcatgttcac
    17641 gtctgactaa gccacgattt tccattcttg ccaacatttc tgccagcgtt gctttggtgc
    17701 tgactgctgc ttctatcagt gccacctgtt cgataccagg cttatcagca atggcgcgca
    17761 tcaccgcgta ctgtggtttg gtcaggtcag gtaactcgtg ctgccagcga gctgtatgct
    17821 gctgaaaaag ctgtcgtaac tgatggaacg ctttatttcg taacgccatg taaactccgg
    17881 gctaattctc tgtcgctatc atagcggttt ttcagtgacg aagaagatgt tgttactttt
    17941 caatccttgc cgctgtggaa atgaaggagt atgttaataa taacgttcgt atacgaacaa
    18001 ttaagagagt aagcaatgaa actgatcgtc gggatgacag gggctaccgg tgcgcctctt
    18061 ggtgtggcat tactgcaagc gctgcgggag atgccgaatg tcgagactca tctagtgatg
    18121 tcgaagtggg cgaaaaccac gattgaactg gaaacgcctt acagcgctcg cgatgttgcc
    18181 gccctcgcag acttcagcca taacccggcg gatcaggcgg cgactatctc ttccggctcc
    18241 tttcgtaccg acggcatgat cgttattcca tgcagtatga aaacgctcgc cggtatccgc
    18301 gctggttacg ccgatggcct ggtggggcgc gcggcggacg tcgtgctcaa agaaggccgc
    18361 aaactggtgc tggtgccgcg tgaaatgccg cttagcacaa tccatctcga aaatatgctc
    18421 gcactttcgc gcatgggcgt ggcgatggtg ccgccgatgc ctgcctttta taaccatccc
    18481 gaaacggtag atgacattgt ccaccacgtg gtagcccgcg tgctggatca atttggcctc
    18541 gaacatcctc acgccaggcg ctggcaagga ttgccgcagg cccggaattt ttctcaggag
    18601 aatgaataat ggcatttgat gatttacgca gctttttaca ggcgcttgat gaccacggcc
    18661 agttactgaa aatcagcgaa gaggtgaacg cggaaccgga tctggcagcc gcagccaacg
    18721 ccaccgggcg tatcggcgac ggcgcgcccg cgctttggtt tgataatatt cgcggcttta
    18781 tcgatgcccg cgtggcaatg aacaccatcg gttcctggca gaaccacgcg atttccctcg
    18841 gcctgccgcc aaacaccccg gttaaaaaac agattgatga gtttatccgc cgctgggata
    18901 acttccccat tgccccggag cgccgcgcca atccagcctg ggcgcagaac accgtcgatg
    18961 gcgacgagat caacctgttc aatattctgc cgctgtttcg tttaaacgac ggtgatggcg
    19021 gtttctatct cgataaagcg tgcgtggttt cccgcgatcc gctcgacccg gataacttcg
    19081 gcaagcagaa cgtcggcatc taccgcatgg aagtgaaggg caagcgtaag ctcggcctgc
    19141 aaccggtgcc gatgcacgat atcgccctgc atctgcataa agcagaagag cgcggtgaag
    19201 atctgccgat tgccatcaca ctcggtaacg atccgatcat caccctaatg ggggccacgc
    19261 cgctgaaata cgatcagtct gagtacgaaa tggcaggcgc gctgcgcgaa agcccgtacc
    19321 cgatcgccac cgccccgctg accggttttg atgtgccgtg gggctcggaa gtgatcctcg
    19381 aaggggttat cgaaagtcgt aaacgtgaga ttgaagggcc gttcggtgag tttaccggcc
    19441 actactccgg cgggcgtaac atgaccgtgg tgcgtatcga taaagtctct taccgtacca
    19501 aaccgatttt cgaatcgctc tatcttggta tgccgtggac cgaaatcgac tacctgatgg
    19561 ggcctgccac ctgcgtgccg ctgtatcaac aactgaaagc cgagttcccg gaagtgcagg
    19621 cggtaaacgc catgtacacc cacggcctgc tggcgattat ctccaccaaa aaacgctacg
    19681 gcggctttgc ccgcgcggtg ggcctgcgtg cgatgaccac gccgcacggt ctgggctacg
    19741 tgaagatggt gattatggtc gatgaagacg ttgatccgtt caacctgccg caggtgatgt
    19801 gggcgctctc ctcgaaagtg aacccggcag gggatttggt acagttgccg aatatgtccg
    19861 tgctggaact cgaccctggc tcaagtccgg cggggatcac cgacaagctg attatcgacg
    19921 ccaccacgcc tgtcgccccg gacaaccgtg gtcactacag ccagccggtg gtggacttac
    19981 cggaaaccaa agcctgggct gaaaaactga ccgctatgct ggccgcacgt caataaggag
    20041 aacaagatga tttgtccacg ttgtgccgat gaacagattg aagtgatggc gaaatcgccg
    20101 gtaaaagatg tctggatggt ctaccagtgc cagcattgcc tctatacctg gcgcgatacc
    20161 gaaccgctac gccgcaccag ccgcgaacat tatcccgaag cgttccgcat gacgcagaaa
    20221 gatattgatg acgcgccgat ggtgccgagc atcccgccgc tgctggcgga aggtaagcgc
    20281 taaataaaag gtccgatgcc catcggacct tttattaagg tcaaattacc gcccatatgc
    20341 accaggtaat taagaatccg gtaaaaccga gaatggtcgt taacactgtc caggttttca
    20401 gcccgtctgc taccgacaac cccagatatt tggtcacaat ccagaaccct gagtcattaa
    20461 tatgtgacgc gccaagccca ccaaagcagg ctgccagcgt caccaatacg cactgaattg
    20521 gattcaatcc catcaccgct tctgagagta acccgccggt tgtcaggatt gctacggttg
    20581 ctgacccctg cgatgcacgc agcgccagtg aaataataaa tgcggctggt aacagaggca
    20641 ggtcaatcat ttgtaacatg ttggcaaggg ctttaccgac gcccgattcc accagcactt
    20701 tgccaaatac ccctccagca ccagtaacca aaatcactac cgccgcagtg ggaagggctg
    20761 agcccataat gtcgctggtg tgttgtaagc tccagccgcg acgtaaagcc aataaccaga
    20821 atgccagcac cagcgcaatc attagagcta ccattggtga gccgatcagc tgtagcgtac
    20881 caagcagggg atgcgaaggc ggcatcagtg ttgcggaaac cgtacccgcc atgataatcg
    20941 cgataggaat aacaattagc gaggtgacca gcgcgacgcc cggtggattt attttatcgc
    21001 ttaattttgt cgcgccttcc tcactggccg gagccagttg catctgttcc agtacctcga
    21061 ccgacatcgc atattggcgc ttattgatta ttttcgctgc aaagtagcca acaatcccta
    21121 cgggaataga aatcgcaata ccgatgatgg ttagccagcc gatgtctgcg tggagtaacc
    21181 ccgctgcggc gacagggcct ggatgaggcg gtaccgccac gtgaacagtg agcatgatcc
    21241 cagcgacagg cagaccaaat ttgagtggtg atattttggc aaccttggca aaaccgtaaa
    21301 tgattggcgc aagaataata aagccgacat caaagaagac gggaataccg aggaagaacg
    21361 ctgccagagt cagcgcagcg atagttcgtt tgtcgcctaa cttgcgactg aaataattag
    21421 ccagtgactc tgcaccacca gagtgttcga tcatacgccc cagcatagcg cccagaccaa
    21481 taataatagt gacggaacca agcacaccgc ccatcccggc gatcatcact ttacccactt
    21541 cgcccgccgg tatacccgcc gcaagtgcga ctaacaggct gacgaggagc agagcaacga
    21601 atggttgtac ctttgccttg atgaccagca gcaacagcat gattacgcca gttaacgcaa
    21661 tgcataacaa tgtaattgtg gacatgggaa accctgtctg aaagttatag ttaacctacc
    21721 ccatccgtag atggggggat gtatgggtac gttgtaatta gggatttaac gaattagcgc
    21781 caggcgtcaa accagccaag tccttcttcg gtgaggccac gaggtttgta ttcacaaccg
    21841 atccagccct gatatcccac ttcatcgaac aggcggaaca gccacggata gttgatttct
    21901 ccatcgtccg gttcatgtcg atcaggtagt ccggcaattt gtacgtgcgc atatttcccg
    21961 gcgtagtcgc ggattaaatg cgtcaggttg ccatctactt tttgcgcatg gaaagtatct
    22021 agttgaataa aaacgttatc tcgcgcaacc tcttcaacaa tagccagtgc ctgatactgg
    22081 ctggagaaga gataatgagg cttaacgccg gggctgagtg cttcaaccaa tattcgcttg
    22141 ccgtgtggcg caaagtggtc ggcagcgtag cggagattat caataaatac tgcccggtac
    22201 cgttcagcat cttcgccagc gggcacgacg cctgccatca catggacttg ttcacaattg
    22261 agcgccaatg catattccag tgccaggtcg atgtctgcac gtgcttcgtg ctcacgtccg
    22321 ggaagggcgg ataatcccca ttctcccgca ttaatatctc cgggagcggt attgaacagc
    22381 gccagcgtca ggtggttttg cgccagttgc tgctggattt gctgggggga gtagtcatag
    22441 ggaaacagaa actccacagc atcgaacccg gcttttcgcg ctgcggcgaa gcgttcaata
    22501 aaaggcactt cggtgaacat catggataaa ttagctgcaa aacgaggcat tgcattaact
    22561 ccttaattcc gcaatttcac ctgcggtcag ataacggatc gggcggtcac cgagaataaa
    22621 aatcagcttt gccgtttcct ctagctcttc catattgttg gcggcttctt gcaggctttc
    22681 accgcaaacc actgggccat gatttgccag taaaaaagcc tgattgtctg ctgccagttc
    22741 cgccagatcc tgtgcgatgc gtttatcgcc cggtcggtaa tagggcacca gcgggacgtt
    22801 tcccatccgc atcaccacgt atggtgtgaa cggccgaata acgttattgc tgtccagccc
    22861 ttccaggcag gaaagcgccg tcgaccacgt actgtgcaaa tgcactaccg ctttacagcg
    22921 cggattgttg cgatacagcg ccagatgaaa gagcacctct ttcgagggtt tgtcaccact
    22981 taaccattcg ccatccgcgg tgactttgga aagccgctgc ggatcgagat tgcccaggca
    23041 tgaacctgtc ggtgtcgcca gtaaattccc gtcaggtaaa agcagcgaca gattgccagc
    23101 cgaaccagtt gcatatccgc gctgaaagaa tgaactggca atccgcgtca tctcctctcg
    23161 caaagactgc tctacttttg cgaaatcgct catgataaaa actctctttg ggctcgtgaa
    23221 aaaaaggctt catcaccgaa gttgccagat ttaagggcga gtgagacagg cttatccagt
    23281 gcgtttaccc acggcacgcc gggggaaatg gttgggccaa tatgaaaacc ttttatgccc
    23341 aggctctgtg tgactacgcc ggaggtctca ccgcctgcga caataaagcg tgtcacgcct
    23401 tccgctgcta accgcgccgc tagttgagaa aacagtgttt ccactgcctg actggctttt
    23461 tgtgcaccgt attgctgttg aattgctgcc aatgcgtcag tgctggcggt ggcaaaaacc
    23521 agtggagcaa gtacactttc ctggcccaga acccactctg ccagctcgtg tgcataagcg
    23581 gccagagttt cggttgagag gcagcgtgcc acatcaacct cacgggctgg tgcaatttga
    23641 cggtaatgtg ccacctggcg gttggtcatt tgagagcatg aaccggagag cactacgccg
    23701 cgcccagcga gcggacgccc tgcttcgcga gcctggttac cgttttcttg cgcccactgc
    23761 cgggcaaggc caatcgccag accagaaccg cccgttacca gtggggtatc gcgcaaggct
    23821 tctccctgaa tttccagatg gtgttcggtc agcgcatcaa gcaccgcgta gcggtagccc
    23881 tcttgctgta agcgagccag ctcctgacga acggcatcca caccttgttc gaaaacatgt
    23941 gccgaaacga cgccgcagcg ccctgtggat tgcgcttcaa ccagacgggg aagatagctg
    24001 tcggtcatgg gatttaccgg gtgatggcgc atcccggatt cggccagcag ttgattcatt
    24061 acgaacaaat acccctgata aaccgtacgt ccgttgaccg gcagggccgg agagaagacc
    24121 gtaaacggcg tgtcgagagc atccattaat gcatcggtaa ccgggccgat attacctttc
    24181 gccgaactgt cgaaagtaga gcagtatttg aaatagatct gtttgcaacc ttgctgttgc
    24241 aaccagctca gagccgccag cgattgctgt gtggcttcaa ccaccggaca ggagcgcgtt
    24301 ttcaggctga tcaccagtgc gtcgattgct tccggcattt tacctgttgg aacaccgtta
    24361 atttgtaccg ttggtagacc gttttccacc agaaaactgg cgatatccgt cgcgccggta
    24421 aaatcatcgg cgataacgcc aatcttgatc atgatttcgc tcccggtagg gtgatgccag
    24481 agaaaatctt gataactgcg ctatcgtctt ctttcccgta acccgcgtta ctggcgctgg
    24541 tgaacatatt caatgctgtt gaggccaatg gcagcgggaa gtgcagggct ttggcggtat
    24601 cggcaaccag accaagatcc ttaacaaaaa tatcgacggc tgaatgcggg gtgtaatcgc
    24661 catccaccac atgacgcatc cggttttcga acatccatga atttccggcg gcattggtca
    24721 cgacgtcata catcacatcc agcgggatcc ccgcacgggc tgcaagtgcc atcgcttcgg
    24781 ctccggcagc aatatgtacg cccgctaaca actggtgaat aatttttacg gtcgaaccta
    24841 gtcccggttc tgcacctatg cgataaactt ttccggcaac ggcttccagc acgggtgcta
    24901 gtcgttcaaa ggcaatatcg ctaccggagg ccatgacagt catttcaccg ttagcggctt
    24961 ttactgcacc accagaaact ggcgcatcca gcatttccag atcgaatcca gccagagcgg
    25021 tagcaatttc ttgcgcatca gcactggcga tagtggaaga aaccattact gccgtaccgg
    25081 gtttcagatg ttgtgcaacg cctgtttcac caaacagcac ctgtttaacc tgggccgcat
    25141 tgaccaccag caccagcaga gcgtccagtt tttcggcaaa cgtcgcggcg ttatcagaaa
    25201 ctccgcaagc acctgcctct ttcaacgtag cgcaggcatt gctgttcagg tctgcgcccc
    25261 aggtagaaag acctgcgcgg acacatgaca gtgctgctcc cattcccatt gaccctaagc
    25321 caacgatacc gacatgaaac tccgatcccg ttttcatctg ctctccttgt taatttaagt
    25381 gatattttgt ttgatgttgt gaatataagc gcggaaagat aacgatatgg tgagctgatt
    25441 cacaaaaatt aacattgtat gttattttat gtgaactaag cgttagttgc gccgcgcacg
    25501 tttcgcaggc aaatagcgta gaatgtcagc aggacaaagg aaggagcaaa agttgatacc
    25561 cgtagagcgt cgccaaatca tccttgagat ggtagctgaa aaaggcattg ttagtattgc
    25621 tgaactaacg gacagaatga atgtgtcaca tatgaccatt cgtcgggatt tacaaaaact
    25681 ggagcagcag ggagccgttg tgttggtgtc cgggggcgtc cagtctccgg gacgggtggc
    25741 gcatgaacct tctcatcagg taaaaactgc gctggcaatg acgcaaaaag cggctattgg
    25801 caagctggcg gcaagtcttg ttcagccggg acgttgtatc tatctggatg cgggaacgac
    25861 cacgttagcg attgcacagc atctgactca catggagcca ctcacagtgg ttactaacga
    25921 cttcgttatt gcggactact tgctcgacaa cagtaattgc acaattattc acactggcgg
    25981 cgcagtgtgt cgggaaaacc gttcctgtgt tggggaagcc gctgcgacca tgctgcgtag
    26041 cctgatgatt gatcaggctt ttatttctgc atcgtcatgg agtgtgcggg ggatctctac
    26101 gccagcagaa gataaagtca cggtgaaacg ggcgattgcc agtgccagcc gccagcgagt
    26161 tttggtctgt gatgcgacga aatatggaca ggtggcgaca tggctggcgt taccattaag
    26221 cgagtttgat cagatcatta ctgacgatgg tctgccggag agtgccagcc gcgcgctggc
    26281 gaagctggat ctctctttgc tggtagcgaa aaatgaataa tgacctgcaa taacgccggg
    26341 ttactcatgc ttcacagaag aagcatgaga ctactttatt ttataaaatg acagccgccc
    26401 gcttttcggc gatccggtat c
//
LOCUS       DAZKJR010000068        26044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKJR010000068 DAZKJR010000000
VERSION     DAZKJR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.068"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(42..653)
                     /locus_tag="ACHOWS_002801"
     CDS             complement(42..653)
                     /locus_tag="ACHOWS_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0048350.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDECGKIENNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            810..1076
                     /locus_tag="ACHOWS_002802"
     CDS             810..1076
                     /locus_tag="ACHOWS_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0048351.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            1087..3177
                     /locus_tag="ACHOWS_002803"
     CDS             1087..3177
                     /locus_tag="ACHOWS_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFQ0048352.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            3249..4181
                     /locus_tag="ACHOWS_002804"
     CDS             3249..4181
                     /locus_tag="ACHOWS_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0048353.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRTFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            4184..4405
                     /locus_tag="ACHOWS_002805"
     CDS             4184..4405
                     /locus_tag="ACHOWS_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048354.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            4418..4672
                     /locus_tag="ACHOWS_002806"
     CDS             4418..4672
                     /locus_tag="ACHOWS_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048355.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            4674..4955
                     /locus_tag="ACHOWS_002807"
     CDS             4674..4955
                     /locus_tag="ACHOWS_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFQ0048356.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            4952..5224
                     /locus_tag="ACHOWS_002808"
     CDS             4952..5224
                     /locus_tag="ACHOWS_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048357.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            5229..5522
                     /locus_tag="ACHOWS_002809"
     CDS             5229..5522
                     /locus_tag="ACHOWS_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048358.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            5534..6064
                     /locus_tag="ACHOWS_002810"
     CDS             5534..6064
                     /locus_tag="ACHOWS_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFQ0048359.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            6162..6704
                     /locus_tag="ACHOWS_002811"
     CDS             6162..6704
                     /locus_tag="ACHOWS_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048360.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFVGDGPCFIARPDESCPEGPTIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            6708..7241
                     /locus_tag="ACHOWS_002812"
     CDS             6708..7241
                     /locus_tag="ACHOWS_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048361.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            7241..7756
                     /locus_tag="ACHOWS_002813"
     CDS             7241..7756
                     /locus_tag="ACHOWS_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048362.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            7760..8311
                     /locus_tag="ACHOWS_002814"
     CDS             7760..8311
                     /locus_tag="ACHOWS_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFQ0048363.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDAVLRELTP"
     gene            8308..8493
                     /locus_tag="ACHOWS_002815"
     CDS             8308..8493
                     /locus_tag="ACHOWS_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048364.1"
                     /translation="MIKRLVFFAIFVPVLGVGFIFAITGNLSMMPDIWFFIRMSLFLF
                     IMNLLIDIYIRITGKHK"
     gene            8532..8864
                     /locus_tag="ACHOWS_002816"
     CDS             8532..8864
                     /locus_tag="ACHOWS_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048365.1"
                     /translation="MSIELRSSYEYRKFLIAGGMKPEDVEKIVSFMDKECDKRDVSEI
                     IMDDMILDSVVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIQMILS
                     SMEDKANE"
     gene            8857..9054
                     /locus_tag="ACHOWS_002817"
     CDS             8857..9054
                     /locus_tag="ACHOWS_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048366.1"
                     /translation="MNSLPAGWARPLMAKKHHFFKTGDNISICRRWLYLAHNREPDTF
                     ESPDDCAECRRRLNKEKDNGQ"
     gene            9044..9340
                     /locus_tag="ACHOWS_002818"
     CDS             9044..9340
                     /locus_tag="ACHOWS_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048367.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            9337..9846
                     /locus_tag="ACHOWS_002819"
     CDS             9337..9846
                     /locus_tag="ACHOWS_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFQ0048368.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            9916..10341
                     /locus_tag="ACHOWS_002820"
     CDS             9916..10341
                     /locus_tag="ACHOWS_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFQ0048369.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            10413..10913
                     /locus_tag="ACHOWS_002821"
     CDS             10413..10913
                     /locus_tag="ACHOWS_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFQ0048370.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            10948..11376
                     /locus_tag="ACHOWS_002822"
     CDS             10948..11376
                     /locus_tag="ACHOWS_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048371.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPGDVADRVRRAVCEIRACEAGADPR"
     gene            11360..11578
                     /locus_tag="ACHOWS_002823"
     CDS             11360..11578
                     /locus_tag="ACHOWS_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048372.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            11589..11816
                     /locus_tag="ACHOWS_002824"
     CDS             11589..11816
                     /locus_tag="ACHOWS_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFQ0048373.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            11797..12105
                     /locus_tag="ACHOWS_002825"
     CDS             11797..12105
                     /locus_tag="ACHOWS_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFQ0048374.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            12102..12392
                     /locus_tag="ACHOWS_002826"
     CDS             12102..12392
                     /locus_tag="ACHOWS_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0048375.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            12395..12976
                     /locus_tag="ACHOWS_002827"
     CDS             12395..12976
                     /locus_tag="ACHOWS_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFQ0048376.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKV"
     gene            12976..14640
                     /locus_tag="ACHOWS_002828"
     CDS             12976..14640
                     /locus_tag="ACHOWS_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048377.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            14640..16229
                     /locus_tag="ACHOWS_002829"
     CDS             14640..16229
                     /locus_tag="ACHOWS_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFQ0048378.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVRDQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALSARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            16213..17544
                     /locus_tag="ACHOWS_002830"
     CDS             16213..17544
                     /locus_tag="ACHOWS_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFQ0048379.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAARVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNLMYIVENQDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(17546..17625)
                     /locus_tag="ACHOWS_002831"
     tRNA            complement(17546..17625)
                     /locus_tag="ACHOWS_002831"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            17666..18139
                     /locus_tag="ACHOWS_002832"
     CDS             17666..18139
                     /locus_tag="ACHOWS_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFQ0048380.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            18316..19440
                     /locus_tag="ACHOWS_002833"
     CDS             18316..19440
                     /locus_tag="ACHOWS_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFQ0048381.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            19440..20387
                     /locus_tag="ACHOWS_002834"
     CDS             19440..20387
                     /locus_tag="ACHOWS_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFQ0048382.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAINRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGINAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            20431..20784
                     /locus_tag="ACHOWS_002835"
     CDS             20431..20784
                     /locus_tag="ACHOWS_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFQ0048383.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVSPEQLARLEAEPCLTVRILQTPADDAGSVAGVVHAVAGTDLAEAGQDTPR
                     KKTGNKAKQAKKARA"
     gene            20781..21200
                     /locus_tag="ACHOWS_002836"
     CDS             20781..21200
                     /locus_tag="ACHOWS_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFQ0048384.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            21197..21757
                     /locus_tag="ACHOWS_002837"
     CDS             21197..21757
                     /locus_tag="ACHOWS_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFQ0048385.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            21758..22003
                     /locus_tag="ACHOWS_002838"
     CDS             21758..22003
                     /locus_tag="ACHOWS_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFQ0048386.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            22000..23502
                     /locus_tag="ACHOWS_002839"
     CDS             22000..23502
                     /locus_tag="ACHOWS_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFQ0048387.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            23511..23876
                     /locus_tag="ACHOWS_002840"
     CDS             23511..23876
                     /locus_tag="ACHOWS_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFQ0048388.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            23891..24367
                     /locus_tag="ACHOWS_002841"
     CDS             23891..24367
                     /locus_tag="ACHOWS_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFQ0048389.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            24494..>26044
                     /locus_tag="ACHOWS_002842"
     CDS             24494..>26044
                     /locus_tag="ACHOWS_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0048390.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPS"
BASE COUNT         6195 a         6537 c         7557 g         5755 t
ORIGIN      
        1 ctatatgtat ttacagcacc acctcatgat agcgatggcg cttaaccaac aacatttttc
       61 tttactgtca ctgcccctga ttcctgggtg gcgttattgc tcgagttgta cttctcaata
      121 acgcgcaaaa tctctttgtc tgtttcttca cttcccgcca ttcctatctt tattgcattt
      181 tttagtacgg gacgtaatgg caaggcatca atgatgcttt ctaccgcatc ctggctgatt
      241 gtagatgtat tcattgctgc cagcctgaca aggttgttag taccttctcg cctgatgaat
      301 gtaaggatta attgcttctc ttgggggctt agagagctaa gcgcctgatc aatacccgaa
      361 cctgaatcgc aattgttttc gatttttccg cattcatctt cgttcttgcc tgtggccaac
      421 cattcgagtg aacaattaca ggctttagct attaaggcca gtttatccaa aggagggaag
      481 tttttcccct tcctgtaatt tcttactgtg gcttcagata gtccacaaag ggtcgccagt
      541 tcgacgtttg tcatgtttcc catagccata gctaagcgtt ctgaaaatcg gtttattcct
      601 tccatatcaa taaaccgttc cgcactggat acggtttttt ctgccttatc catataaccc
      661 cctataaaac aaagaaatta tcaacatcgc atttaatttc gatgtgctcg caaaaaaccg
      721 ttgatatcga attattttgc gattatgatt tacctaaatc gataaccggt tttgattatc
      781 ggttcaggta aacatgagag gatcacacaa tgagggtatc cagacaagca tcgaataatt
      841 tgtttgttcg agaggggtgt gactggtctg ctaaagcggt atatgcagcc cttgaaaaca
      901 aaggcacaga cctccctgaa ctggaaaggg agttaggatt aaaaagcgga tcaatgcgca
      961 atgtgtttta ccgccagtgc cgtaaatacg aagccgccat cgccgaaaaa ataggggttg
     1021 aacctgctgt aatctggccc agcagatacc aggtggacgc cagagaagtg gcttgatggg
     1081 ggatgtatgt gggtaacagc aaaagaatgt gttggtcttc ctgatttacc aatgatggaa
     1141 cataacattc gccatcgact cacaaaagca gctgcgggaa atgcagccat gttacgcaaa
     1201 cgtaagggga caaaagcaat cgaatttaat atcgattgtt tgccttctgg cgcgcaacaa
     1261 gtattgcgtg aacgttatgc ccttcaactg atgacgcaaa aagctgatga atcaccggct
     1321 ccggtggtga caaaggccag acgctcgcct gccgtggttg atgcggtgga ggcatatcgc
     1381 ggatcaccac aactgatggt tgaacgcctt aatgccctga ctgaaaacca gcgccaggtg
     1441 gctgatgcac gaatcgcgat cgtcagcgaa gtgatgaaag tcgcgcaaca acccggtttc
     1501 agctgtgcga aagctatccg gtttatcgtt gacaacctgg cacgttcgca actggacgag
     1561 cgcattgtgg caatggttga gacggcgaac gccaaaaagg gaaacagccg tgcgttgagt
     1621 gaaataacgc tgaaacgctg gattgcggcc tttaacaagg cgcagaacgc cgctgaacgc
     1681 ctgcttttac tggcaccggg taagcgccag gaaataaaag ccgaagatat taactggctg
     1741 cccgaatttc tggcgcagta tcgccagtca aacggccgac caatgaccga ggcttacgag
     1801 gattttgtcg ctgaatggca gcaccggcac gctgatgagc cttatatgct cgatatcatg
     1861 ccgtcttatg acaccattcg ccgcgcaatg aagaaactgc cggaagtggt gaaacaaaaa
     1921 ggccgggtga ccggcagtga ataccgccag cttgagggat tcacgcgccg cgactggtcc
     1981 agaatgccgg tgaattatgt ctggattggt gacggtcacg gcatgaagct gaaatgcgca
     2041 cacccggttc acggtcggcc atttgcaccg gaagtgacct ttgttatcga cggtggcacg
     2101 cgctttgtgg tgggctggag ccttgacctg gctgaaaatg ttttcgccgt agccggtgcc
     2161 atacagcacg gcattcgcca tcacggcaaa ccgtttctgt attactcgga taacggctcc
     2221 ggggaaaccg ccgacatcct ggataaggag gttgtgggga tactgccgcg actggggatt
     2281 aatcacccga ccgggattgc cggtaatccg cagggacgag gcattatcga acggcttaac
     2341 cgcacattac cgatgcgcat agcccgtaaa taccgcacct atttcgggaa aggtgcagat
     2401 cgcgagacgt tacgcaaaac caaccgcgat ttacgctcgg catttactgc cctgcaacag
     2461 ggcaaacggc tgaacgcccg gcagcagtca gcgatgcgtg atttaccgtc ctggtctgaa
     2521 ctgattgatg ccattcgtga cggtgttgag tggtacaaca accggccgca cgatgaatta
     2581 ccggtgaagc cgaacggcaa gcattacagc ccggcggagt tcagaaaaaa acgcctggcg
     2641 gaagaggaca cggaaattga atggctgtcc gatgttgagc tgcgggatat gttccggccg
     2701 atggtggaac gccctgtaag acgctgtgaa atacgctggc tgaataatat ttactacgcg
     2761 cccgagctgc gtgacgagca tggtcgtaag gtgcttatca gctatgacat tcatgatgcc
     2821 gaacgaatta ccgtacgtcg cctggatggc agcgtgattt gcgaggcggt atgggacggt
     2881 aataaacgcg aagccttccc ggttagcgcg gaatactaca aacagcagca acgccttaaa
     2941 ggcatgcgta aacgcgcaga ggaaaaaatc cgtgatgccg aggatgaggt tgtcaacgtg
     3001 ctggagcaca agccgcagga gccctggctg gaaaacatat accgccctgt gggtaatacg
     3061 gtgaccgttc agcaaccggt cgctgacgat gaacctgatg aagaatacga gcgtaatttc
     3121 cagcggggat tgcagttgct ggaagcgaaa ttaaaagaaa atgacccgct ggcctgaaat
     3181 aaaaaaataa cccgagcggc gactcaggtt atttgattaa acaaggtatc aaatgagagg
     3241 ttaataatat gactgatatt aacgatgtaa tcaagaccat tgatgaactt attgatggcg
     3301 gcgtactgac gcagtatgcc atcgccagag aggcgggaat ttccgacggc acattatcgg
     3361 ctttccgtaa gggaaaatat aaaggcgata acgctgctgt ggctgcttcc ctgcgttcct
     3421 ggtatgagaa ctggaataaa caaagcgcac tgccggaacc gccgcagttt gtggaaacgc
     3481 agacagtcca ggagctgcgc gcactgtttc aggcggttcg tctgatgggc tgtattaacg
     3541 ttattgtggg cgtaccgggt gtgggtaaaa cggccactgc ccgtaattac tgccaggagc
     3601 aaccaaacac ctggatgatc accctgtcac ccgcgcactc cagcgtcacg gagtgtctgc
     3661 tggagctggc cgatgcgctg gggattgatt acacccgcgc gaacaaaggg gcattatccc
     3721 gcgccatccg ccgtcgcctg atgggaacgc gtggactggt tattgtggat gaggcggatc
     3781 atcttggtat tgacggtctg gagcaactcc gggcaattca ggacgccacg gggatcggga
     3841 tggtgcttat tggtaacccg cgcggattgt ttaaaggtgg acgccgcacc tttgatgatt
     3901 tatcgcgcct gttcagccgt cttgcccgta caaaacaact tcgcaaggcc aaaaaggcgg
     3961 atgtgctggc cattgccagg gcgtggggga tcagtggtga ggccgagctg gccgtcatgc
     4021 aggctatcgc tgaaaagccg ggagcgttac gcgttctgac acatacgctt aaccaggcgt
     4081 ggctcaccgc cagcggtgaa ggcgcggcgc tgacagaaaa acatattaat gcggccttta
     4141 aagaggttta taccaaccct gaattactct cacaggtgtg attatggcgg tatttaatat
     4201 tcctgatatt tacggacgct tttacttggt taatttcgat aacgtgaagg tgatttcact
     4261 ggccgaaaat aaagaatgtg gcgatttact ttttgaattt aatgaccgca cacgaatggt
     4321 gatatctgcc ggacttgatc gagaaggtgc gacagacgtt tacagcggaa tatgccgttc
     4381 tgttggtgcg aaacaagtca gctaaatgag gtgttatatg aatatgcaat cctgcggtaa
     4441 caaaatgaat ttattcgact ccctgaacag cgcgcgccgt ctgaccgaac ttgccggtgc
     4501 ggtactggaa cgcagtaagc gctatccgca acgttttgca ctgaaaacca cgccgccggt
     4561 aggcaacgtg cagggaaccg gtgaaattga aatcaccata cagaccaacg gcctgcgccg
     4621 ccgtgtgaag gccacccgca tcagcggctg cacggtttac tgggaggtgt gaggtgaaaa
     4681 aaaatctcat tgcatgggcg tggtcgagtg gtcttattga gtttggttac gtcctgccgg
     4741 aaggtgcatt gccgatagtt gccggaaagc ctgccacggt acggcatgtg attgaggtta
     4801 tggcgcgtca tggacgtgat gaacaggagc agttactggt tccggggata ccggaagcgg
     4861 tgacggagga agaagccttt aatgccatga ttcggttctg ccgtgaggtc agacgccggg
     4921 tcagttatcc aaacagaacg aggaccagag gatgagtaaa gtcgtacgca ttattttcga
     4981 atacaaggag cacgttatcc ataaaaacgc tgatggaaca gtgcgcatgg gggtaagtct
     5041 ggacatacgt tcaaccggga taaagcagaa aggtgatgga cccgccatga tttttggggt
     5101 ggttatgctc gcggaaagca gagactttgc tgaacttgtg gcaatgaaag ccagtgcgct
     5161 catgaaagat atgagcatgc gttccggggt tattaaaggt aatgaattta atcagcaggg
     5221 gtaattccat gagcaaagta cgcgttattt ttgaatttaa gcatgtttcg catgacgaaa
     5281 aaccggcagg caatgactgt gttgaagtgc atgaaaagat tggtgtggat gtaaaaacag
     5341 aacgtgatac gaataacagg ccgacgtcac tctgtgacgt ttatgcaagt attctccagt
     5401 atcacagtcc tgaaattatt cagtttctct cagcggaatt tcaggcatct gtacaggctt
     5461 ttggggcgga tgccatcatt aaacgccacc gcgtgcataa agcatcaggc acactgcaat
     5521 aaggaaaaac aaaatggcaa aacgcgttac aaaattaaag gccgcagcag aggcggcacc
     5581 gcagacccgt gaagaggtca gccgcgatat ccgcaccctg ggcgatattc agcgagaggc
     5641 gctgcggctg gaaacggcga tgaatgatga agtggcagaa atcaccgccc gttatacgcc
     5701 gcagattgaa aaccttaaaa aagaaatcaa agtgcttttt aaggggattc aggactggtg
     5761 caaaaccaac cgagatgagc tgacgaacgg cggcaaaacc aaaactgcca atctgaccac
     5821 cggaacggtg tcatggcgac tggggaatcc atcatgcagc gtcagtcgtg atgtggaagg
     5881 tgtgattgaa atgctgcgcc gtatgggcct tgagcgtttt atccgcacga aagaggaagt
     5941 gaacaaggaa gcggtcctgg cggagccgga tgcggtgaaa gggattgccg gtattaaggt
     6001 gaataaaggc gctgaaagtt tttatgtcga gccttttgaa caggacgctg gactgaataa
     6061 ataacaccgc attaatcaat taaatatcac ttcgttttaa ttatggcgct cgcgtcaggg
     6121 gactgcctgc gcctgtaaac tgaaaataag gaacaggata tatggcatat ttttatttca
     6181 aattagaccg tgtgcagaca aataaatatt tcaccaaata tcaacagact gttttaccgc
     6241 tacgcaacag tattcttcgg gcattactga aaaatacagg cgctgccgga ttgcgcttaa
     6301 agccgtttgc catggacgta atcagtgagt tttatttttc tggtgctctg cctgcgggct
     6361 ggcgtaagcg cgatgatgtg gcttttgtcg gggacggacc gtgctttatt gccagacctg
     6421 atgagtcatg ccctgaagga ccgacgattg ccgcaatgat tgaagccgct gaacgcgagt
     6481 taagaaagcg tcctgatttc cttgtctggc tctgtgaaaa gctgggggta atgagaatcc
     6541 cctccatgtt taacatggac tcctggtgga ccccgtcgct ctcccgtgat gccctgtgcg
     6601 tggtgtttaa agtaggcgct tatggcaggg aaataaaagg gggtattcct gaagaatgcc
     6661 aggagattaa acattctgaa tatgtggcgc tgacggagga ataattcatg attgatgcaa
     6721 aagtgcttga aggggttaaa aactggctga gtatttacgg gcgtctgacc tgcggcattc
     6781 tggctgaaaa aatgaatatg ccgccatcct cgatggttta ttttctgcgt gatgcggtcg
     6841 atgccggggt gctgacggaa tgtaacggtt tttatgatat tccgcgtccc cgcccggtgc
     6901 agccggttcg tcgcaaatgc agccaggaag gtgcggctga tgatgttcag tggtgcagct
     6961 tcagaaaatc cctgccgtgg attgaggggc atgatattcc gtcaatggcg tgggaatttg
     7021 ctcagggcgt tctgacctgt gaaaccgttt atgtggtggc tgaagttgat gagcaggcca
     7081 tgaaagaagg cgtgccccag tttgtgatgg cgtatatcga catacgcctg ggtgtcatta
     7141 tctgcggttt aagcggctgg aatatcacct cgcgcgttct gcgttacctg attgttgacc
     7201 ggacggccgc gcctgccggg atatctgcgg aggtggcgta atgttcttta aaacatcaaa
     7261 cccttccgcg ctggccgcgt ggcaaaaata tcagcaggac tgccagaaag ttaaggatga
     7321 ggcaaaacgc cttgaggccg tgctgaatgt tgcgtgccgg tcggtatttg tatccggtat
     7381 cagtggcttt tgttttaaag ggctgcgctt tatggatgac aaatatcctt ttcatcgcga
     7441 cttatggcga aaaccgactg cgtcgaatgg ctggagctgc acaccgcgca catcacgtat
     7501 tcccaaagcc ctgcgcgttg cctctgacga acttaacagt ctgtggcgtg aatattcgcc
     7561 cgtcacgtat gccagaaccg atgcactgtt gttctggctg ggtattgatt tctcggcaat
     7621 catgtttggc cctgtgaagt ggttctgcgt tgacgatgtg atttaccttg agtgcggcgt
     7681 aaaacccgca aatcagaaaa tgaccgaaat tctgtctgat gagttttatg ctgctgaaaa
     7741 gcgagtcagg gggtgatgta tgatgatttt acaacccatg gggcgaaaag gtcaggcacc
     7801 tgctcatgtc cgtgcgtgga cacctgaaga agatgcgctg ctgattgcgc tttatccatc
     7861 caccccggtt aaggatattg ctgtcagggt aaaaagaagt ttctggggtg tacataaccg
     7921 gattgtttta ttacgcggta cttacccgga gttgctcaaa tgcaaacgtc caagatttaa
     7981 acatgatgaa gataaattta tccgaaaaaa tgccagaacg atgaccggta agcaaatagg
     8041 agaatatctg gggcgagacc gggattctgt ccacaatcgg gcgcgatata ttggcgtaag
     8101 catgaaaaaa tacggagaat tgctgccctt cacccgtata cctgacgatg atgttcatct
     8161 tattcgtgaa ttacgggatg ctgaatcacc acgacgtctt accttccggg aaataggcga
     8221 gaaatttgaa ttatccgaga gtacggtgaa ttttatttat caccatcgcc ggactgccga
     8281 ggacgctgta ttacgggagt taacgccatg ataaagagac ttgtattttt cgcaattttc
     8341 gttccggtgt tgggggttgg ttttatattt gcgattacag gaaacctgtc catgatgcca
     8401 gatatttggt tctttatcag gatgtcgctc ttcctgttta ttatgaatct tcttatcgac
     8461 atatatatcc gtattactgg aaaacataaa tgagaaaaat cacgtccttg tcagagttac
     8521 aggagatgaa tatgagtatt gaattaagat cgtcttatga gtatcgcaaa tttcttatcg
     8581 ccggaggcat gaaaccggaa gatgtggaaa aaatcgtttc ttttatggat aaggaatgtg
     8641 ataagcggga tgtgtcagaa attattatgg atgacatgat tctggattca gtcgtggcgt
     8701 taagtccgtt atggattgtg cattctcttg cggaaattgc caaaggcacc gataaacagg
     8761 cagctgttgc tgcccttcag acgcttaatg aaatgcgtat atctccacgc ccgacattaa
     8821 tacagatgat tttgtccagt atggaggata aagcaaatga atagtctacc cgctggatgg
     8881 gcgcgccctc tgatggctaa gaagcatcat ttttttaaaa ctggcgataa tatcagtata
     8941 tgtaggcggt ggttatatct ggctcataat cgcgagccgg atacatttga aagccctgat
     9001 gactgtgccg aatgtcgcag aagactgaat aaggagaaag ataatggaca atagttctct
     9061 gttgttctgg tgcttatata tcacatcctt tttcggcgca tttgttatta cccggtggtt
     9121 atgtcgaaaa atcatctgct tttttgataa aagacatccg gttgaacggg cggctgatgc
     9181 gctcattcag caggccattg tgttatatag cggtgaattt ttctgccgga taacaaccag
     9241 agatggctgg cacataatga ttattccacc aacacaccat gcccggtggg atgaggcaga
     9301 aaaggctttc catgtccgta aaaaggtaaa tacggtatga gaggaaaact gatatccgcc
     9361 attcatgtgg caaagcgcga gcttgccctg gatgatgaga cttatacatc cgcgctgctg
     9421 gcagccaccg gcaaaaccag ctgccgggat atgtcaccgg atgagttatc ccgcgtgctg
     9481 gatgttttca aaaaacgcgg ttttaaagtg cgtcagaacc cggttaaccg ggccttaaaa
     9541 ccgggtacgg tgaccgccaa aattcgcgcc atctggaagg tgatgcaccg gcagggcttt
     9601 atctctgatg gtgcggaaac cgcccttaac cgctgggtga aatcgcagac ggccgcgcaa
     9661 aacggcggcg aaggtgtggc aaactggcag tggctggagc aacaccccgc cctggcctca
     9721 gatgtgctgg agcgtctcaa gcgatggcac cgccgcaaaa tgctggccgc gatgggaatg
     9781 cccgaacgca cgctgatggg gtatgacgcc gtttgcaggc agtatgaaaa atcacttccc
     9841 cgttaactcc aaatcccgcc acaacgcggg atttttattt taaacttacc gggaacgcga
     9901 gaaccggagg ctgatatggc agaaactcag atgagcatgt ttggtggtga cagtgagcaa
     9961 ctgcacgccc ttatcgaccg ccttgatgac atccctgatg atgttctcaa aaagaactgg
    10021 ccgcggaccc tgtcagaact ggttgaagtc accggcgcag aacttcagcg tcaggggatt
    10081 gagccggtac tggccggtaa actggcacgc aaggtggctg cggctcaggc agcctatatg
    10141 ggcggacggg gttattacct gccggtcggg gaatctctct ttgccgagct gcgaaacaat
    10201 gagatatttt cgcgctggga ccggggcgag aaaattgaat cccttcgccg ccattaccgg
    10261 atgtcagaaa cccagattta taccgtcata cgcgaacagc gccgtctgca tctggcaaga
    10321 acgcaaccgc cacttttctg atattccgca aagcctgccg cctctccttc accgttacgc
    10381 tgactcagag aacatcatga gacagcgtaa caatgccaaa actccccgca ccactgcgta
    10441 aaaagctgat tgcccttgtt ctggccggtg ccgggacgtt cacgattgcc acgcattaca
    10501 ccggttactg ggaagggaaa gaaaactcca cgtatatcga tcccaccggt acacccacca
    10561 tctgttacgg ccataccggc ccggatgtga aaccgggtat gaccctgaca gatgaagaat
    10621 gcctggaact actggaaaag gacatgaaat gggcctttgc ggccattgat cggcgtgttc
    10681 aggtgccgct tacccgtggt cagacggtgg cgctggcttc gtggatcttc tgggccggtg
    10741 aaacgaactt tcgcaactcc acgcttctgc gcctgatcaa tgccgggcag atgcccgcgt
    10801 cctgtaagca gtatatccgc tggatttatt caaagggggt gaaactcccc ggccttgagg
    10861 cccgccgttc ggcggatgaa tggttatgtc gctacgactt gccgaaagtc tgaaccgctt
    10921 ctggcgaccg ctcatgatag cgctgctgtg tgcggtactg ctgttacggg gtgtcctgtg
    10981 gctgcggtga ataatgcctt accggccctg attgccgggg cgtgcatggc ggcgctgggg
    11041 attatcgctg tactggttgc cgctatatgg ggaatgcacc agaagacgca gcgccttgaa
    11101 gacaataatc aggtgcttgt gcgtgagcgg gatgaggcgc gtcaggtgct ggcaaatcag
    11161 cagcacaccc tgcaactcat ttcacaaatc agtgaggcgg ccacgaatga aaaacaacag
    11221 aacattcagc acagcgaggg gcagcagagc gttgtccgcc ggtcgctggc ggcagtgcct
    11281 gcggcctctg cccctgttcc tggtgatgtg gctgatcggg tgcgccgggc cgtctgtgaa
    11341 atacgtgcct gtgaagccgg tgccgatccc cgctgaatgg ctggctgact gcctggtccc
    11401 tcctgcgccg gagccgttca cgtttggggc atcggtcact tacaacctgc aactgctggc
    11461 ggtgatcaag aactgcaacg tggacaaggc cagtattcgt cgtctggagg cgcggcgaca
    11521 acatgaattt actgatatgg ccggaacgcc tgctgttccg gcaggaaaga cgaagtaagg
    11581 aaaaaggtat ggatgattca gatcgtgctc aggctgtgat ggagcgggga tcagaacgcg
    11641 ccctttgtaa ccggctgaca cgtaagcgcc gggcaatgga tgctccgggc agacgtgtct
    11701 gcgcggactg tggcggagag atccccgccg cacgtcttgt tgctgtgccg gatgccatcc
    11761 gttgtgtgaa ctgccagaac atcatggagg cccgtcatgt gggtcagcat cgttaaagat
    11821 tatgttgtgc cgatcctttc ggcgacggca accgctggcg ggatattcat ggcgctgatg
    11881 cgcaaaacgt ttgtcccccg cgaggccttt gaaaaactct ccgaccgcgt tgagaaggtg
    11941 gaaacccgcc tgtcgtcact gccgacagaa gccgaggtca accgtctgaa tgtggaaatc
    12001 gtgaccctgc ggggtgaact gaaaaccacg aacgccacgc tccgctctgt ctcctatcag
    12061 aacgaactgc tgctggagca ggcggtaagg aaaaaaacac aatgagtgat ttcattactg
    12121 aagatcagcg tcttgtcatt ctgcggtcgc tggcggatta caacggcgaa ctgggtgaat
    12181 ccgtgctcca ggactgtctg gatgattacg gtcaccgcgt gtcccgcgac accgttcata
    12241 ctcatattgc ctggcttgcc gagcaggggc tggtgcgtaa gcgcacgctc gttaatggtt
    12301 atttcatcgc tgaactgacc ggccggggtc aggatgtggc ggaaggccgc gccaccgttc
    12361 cgggtgtgaa gaaaccacgc gcaagggggt aacgatggat aagccaacgc gtggccgtgt
    12421 acgcaaggtg gacttgctcc ccgacagcat ccgtaagcca ctgctggaaa tgctgcgtga
    12481 aaaacgtctg acgcaggtcc agatacgcga ggaaatcaac cgtctgatcc gtgaggcggg
    12541 cctgccggaa gagcagcagc tttcaccggc ggccatcagc cgggaagcct cccgtaatga
    12601 gctgattgcc cgtaacctgc gtgatttacg cgagcagaca aaagccatga tggccgaact
    12661 gggggacaag ccaaccggtg aaaccactgc attgatcctg gagatgtccc gcgccctgat
    12721 gtaccgccgt ctgcgtgccg cgaccgaatc cctgaacagt gacagcgacg tggatatgcg
    12781 tcttattaag gacatcctcc tgtcagcaca gcgtgcggaa agtgccgccg agcgcagcat
    12841 taagcgtgaa aaagaaatca gggctgcatt tgctgaagag atggcaaacg ccgtcaccga
    12901 cgagctgcgc ggcgtggacg ggatgagtga acagcttgag tcccgtatta aggggattct
    12961 gttgggtaaa gtctgatgaa taacgccgaa gaactctccc ccttactgac gaataccgtc
    13021 tccacccgca aaattgatct ggccggtgaa aaagccctgc tgggcgtgga tgtgccggac
    13081 agccttgatt tgcccggtga tatgccggta tttctggatt atcaggcccg ctggtttgag
    13141 gatgaaagcg aggtctgcat cgcggaaaaa tcccgccgca ccgggcttac ctgggcggaa
    13201 gccgggcgta acgtcattac cgctgcaaaa ccgaagcggc gcggtggccg caatgtgttt
    13261 tatgtggggt caaagcagga gatggcgctg gaatatattt ctgcctgtgc actgttctcc
    13321 cgtgccttta accagctggc agatgctgat gtgtatgagc agaccttctg ggatcgggat
    13381 aaaaaagaag aaattctgac ctacatgatc cgctttccga acagtggatt caaaattcag
    13441 gcactgtctt cccgtccgtc aaacctgcgc ggccttcagg gggatgtggt gattgatgaa
    13501 gccgcgttcc acgagtcact ggatgagctt ctcaaggcgg caatggcgct caccatgtgg
    13561 ggcgcgcgcg tgcgtattat ctccacgcat aacggcgtcg ataatctgtt taatcagtac
    13621 attcaggaag cccgcgaggg gcgcaaggat tacagcgtcc accgcattac cctggatgat
    13681 gccattgcgg acgggctgta ccgtcgtatc tgttatgtca ccggccgcga atggtcaccg
    13741 gaaagcgagc agaagtggcg tgatgatctc tacaaaaacg ccccgacccg tgaggatgcc
    13801 gacgaggaat acggctgtat cccgaaaaaa tccggcggtg cctatattcc tcacgcgctt
    13861 attgaaatgg cgatgctccg cgacatcccg attctgacgt ttgaagcccc ggacgacttc
    13921 atcagccgtg ccgcatggct gcgtgaatcg gaggttttaa cctggtgtga agaacattta
    13981 aaaccgcttt tagaggcgtt aaatccccgt tcccgcttca gcttcgggga agactttgcc
    14041 cgcaccggtg acctctcctg ctttgtgctg ctggaaatca ccgaatccct ggcaaaacgc
    14101 gaggtgtttc gcgtggagct gcgcaacctg ccgtatgccc agcaggagca ggtgatgatg
    14161 tacatcctga cccgcgttcc ggcgctggtc ggtgcggcgt tcgacgccac cggtaacggc
    14221 ggctatctgg ccgaagccgc gctgctggcc tttggcccgg acatcatcga ctgcgtgatg
    14281 ttatcgccga agtggtacgg cgagtggatg ccaaaactga aggccgagtt tgaggatcag
    14341 aatatcctcg tcgcccgcca tcagaccacg cttgatgatt tgcgccatgt gaaggtggtg
    14401 aacggtatcc cgcaaattga caaggggcgt acgaaggacc agaacgcaac ggctgtgaac
    14461 gcccgccgcc atggcgattt tgccgtggcg ctctgtatgg caaaccgggc gtcatacatg
    14521 gagggcttca tcctggatga atcagcctgt caggcgctgc cggagcggtc gcgggccatg
    14581 gagggcggtt atcgtgatga cgatgaggca tatcatgaat ttgatcgggg gtgctggtag
    14641 tgggacgtat aattgatctt gacggaaaac ctttctcctt tgacccggag atgcagagcg
    14701 ccgcgctgga tattccgcag attgccagcc gttatattga acatccggcc tcgggtatca
    14761 ccccgaaccg ggcggcacag tgccttcgcg gggctgaacg tggcgatctg attgcccagt
    14821 ccgatctggc cgctgacatt gaagaaaagg atacccacct ttttgcagag ctgggcaagc
    14881 gacgccttgc cattcagggg gtgccctgga gtattgagcc gccaccgaac gccagcgcga
    14941 atgagaaaaa ggacgcggaa atgctcgacg aatatctgca ttccgccgac tggtttgatg
    15001 ccatgctgtt tgatgccacc gatgccatcc tgaaaggcta ttcctgcatg gagattgagc
    15061 acgggatgct cggtaaaatg cacatcatcc gcgccatccg ctggcgtgac agcgggcatt
    15121 tctgccttaa cccggatgat ttgagcgaac tgcggctgcg tgacggcagc catgccgggg
    15181 tggcgtttca gccctttggc tggatagtgc atcagtcacg ttcacgcacc ggttacggcg
    15241 gcgcaacagg gcttgtcaga acgcttatct ggccgttcat tttcaaaaac tattccgtgc
    15301 gcgatctggc tgaatttctg gaggtgtacg gcctgccgat gaaggtcggt aaatacccgt
    15361 ccggggcaac gccggagcag aaaagcgccc tgatgcgggc ggtgatggat atcgggcgac
    15421 gtacaggcgg gatcatcccg gccgggatgt ctctggagtt tcaggcagcc gcgaacggtc
    15481 aggccgatcc gtttgaaacc atgatttcgt ggggggagcg ttccatctcc aaagcaatac
    15541 tcggcggcac gctgaccacg gaagccggag acaaaggcgc gcgctcgctg ggtgaagtgc
    15601 ataacgaggt gcgccgggaa atcagggatt ctgatttacg ccagctggcc gccacgctga
    15661 accgcgatct ggtgtatccg ctgtatgccc tgaacaccgc ccacgccatt gatatccgtc
    15721 gcctgccgcg tatctgtttc cagacaaaag agccggggga tatcaccaaa atcaccagtg
    15781 cggtgatgca gctcagtacg gggatggata ttcctgatcc ctgggtacgg gaccagaccg
    15841 gtattccaca gcctgccccc ggtgaagcca tcttccgtgt ccgtcagagt ggcaatgaac
    15901 ctgctcagac agacaaagag atgccaccgg aaaaacagga aaagacagag cagacggcgc
    15961 tgtcagcccg actgccggaa gcgaaaagca gcccccgtga tgaactggac gatatggggg
    16021 atgcggtgcc tgcccgccgg ttacaggagg ccatcgaccc ggtactggag ccggtcattg
    16081 atgccatcag aacgcggggg ctggcggagg cactggcaga cctgcctgcc ctttaccgtg
    16141 aaatggatga ttcccgcctg atgacgctgc tcagtgatgc catgtttgct gcggaaatga
    16201 aggggatgct ggatggcaca ggggattgat ttaggttatg ccgccaccct tccctcaaaa
    16261 gaggcggtgg catacttccg cgccaaaggg gcgcatatca gctggaactg gttcgaaaca
    16321 gacgcggatg ttcatgcccg ttcattcacg gcggcaagag tggcacgcct ggacgtactg
    16381 accacactac aggcggaagt gcagcgtgct gttgatgagg ggatttcaca gaaagcattt
    16441 atccgcacac tgacgccccg cctgcaaaag ctgggatggt ggggaaaaca gattgtggtg
    16501 gacagtgccg gtaacgcgga agaagtgcag ctgggcagtc cccgccgtct ggcgctgatt
    16561 tacaacgtga acacccgtgt ggcttacaat gccgggcgtt acacgcagat gatgaacaac
    16621 acggacacgc atccgttctg gcagtatgtg gcggtgatgg acagccgcac ccgcccggcg
    16681 cattccgctc ttaacggtct ggtattccgc tatgacgatc cgttctggaa tacacactac
    16741 ccacccaatg gctggaactg tcgctgccgt gtccggccat tgtctcaggc ccgtctggat
    16801 gcaatggggt tatccgtttc atccggtcag gatcatctct ccacccgcaa tgttgaggct
    16861 ggcgtggata agcagaccgg ggaagtcaga gaaatgccgg tgaccacata ttcagatggc
    16921 accagaacca tgacaccgga tgtgggctgg tcatataacc ccggttcggc ggcgttcggc
    16981 acagaccagg cgctgatccg taaactgatc gaggtgaaaa gcccggcgct gcgggaaatg
    17041 gtggttcagg agatgaacaa cagcccggag cggcagctgg cgttccgcat ctgggcaaaa
    17101 aacatcatga aaacccggcg aggcggtaac gatatccgca cgctgggctt tatgaccgaa
    17161 agcattgcgc aggcggtgga aagccggacg ggaacgccac cggcccgcct gctggcgatg
    17221 agcggtaaaa atgtgctcca tgcggacagc atgaaacatc agaatgacgg tatcgccctg
    17281 acaccggagg atttcgggcg cttaccggca atgctggcaa ggccaaaagc ggtactgtgg
    17341 gacaaacggc acaacaatct gatgtatatc gtggaaaatc aggatggcag tgtgcagatt
    17401 gccgttaatg caccgtacag cctgaagcgt cagccggata aactggatgt gattgttaat
    17461 gcttaccggg ttatcaatat ggataagctg aaatcggata tccggggcgg aatgcttgag
    17521 gtactggaag gagatattga ttaacggtca gtggcggggg tcgaacccgc atacatgatc
    17581 cacccggagg caggcaccga ctttaccagt taagcgtaca ctgaccgcct gcttatttta
    17641 tattcatatt ttatacggag gcaatatgtc atccattgat gccgccgtcg ttgttgacgt
    17701 tacgcgtctc cagcgggtct ttgcccggct tcagtttgtg ggtggcggaa aagacctggc
    17761 ccgcagtgtg gcgtccagcc ttctgtcatc gtcagaaatg gcgtttgaac aggaaaaaga
    17821 gccggacggc gaacgctggc atgactggtc agatccttac cgcaagtggc gtacccgtaa
    17881 gggatacatg cccggcaaaa tcctgacgct gaacggcgat ctggcccgac gcctgaccac
    17941 ggattatggc gacacctggg cgctgattgg atcaaatgag ccttatgcgg ccattcatca
    18001 gtggggtggc ctgccaggta tgccgccggg accggcggcc attggtgcac gtccgtatat
    18061 gggctttgat caggtggcag agcaggagat catggacgaa atcagaaaac gctttaaaaa
    18121 ggccacagaa acgccttaat tgtttaaggt atgcaaatgc attaccttac ccccttcagg
    18181 cgcgtgtcgt gattttctaa cctgtattta acgggcttta aaatctgcgt gaagcgcctt
    18241 tgtatttttc ccctggaagc acttcctgaa atcccgcaaa acccgccctg aaaattcccg
    18301 gcctatgctg ccggaatgaa gacgaaaaac acgcccaaac ttgcttatgc cattctgaat
    18361 gccatcagcc tgtccgcaga cggggacggt gactggtgtc agatcatgcc agccggtcgg
    18421 gtaaaggccc gtgacggtcg cccggaaaaa ccggccgagg gctggctgat caaccatgca
    18481 gccgttgagc gcatggtctc ccgtgttgtg gcgctcaatc agccggtgaa aattgactac
    18541 aaccaccaga ccctgattga gggacatccg gcaccggctg ccggttttgt gatggcctca
    18601 ccggagaatt ttcgcttcag tgaagagcga ggtttcgagg tgcgcccgaa gtggaaccct
    18661 ccggcccttg aacatctgcg taataacgaa ttcccctggt tttcaccggt gattggctat
    18721 gacgagagca ccggcgaacc tgtcgaactt cggatgctgg ctattaccgg tgaccccggt
    18781 ctgaccggca tgaatcctgt cgccgcactg tcggcggatg acctttataa cgccttaaac
    18841 cctcctttaa aggatacctc catgaatgag caattacgcc agttgctgac ggcgctcggt
    18901 ctgaccgtgg ctgacggtga cgaatttacg ccggagctgg gcacggcggc gctgtctgcc
    18961 ctcaccggga tcaaaacccg tgcggatgca cacgacaacc tgaaaacaca ggtcgccagt
    19021 ctgtcggcag agctggaaac cgcaaaaggc acaccggctg gcggcagtat tgatctgacg
    19081 aaatacgtgc ccgttgaaac gtataacgcc ctgcgtaccg aatacgttgc gctgtcggcg
    19141 cagcacggca gcaccacgct ggagcaggtg ctggacaagg ctgaatccga agggcgcatc
    19201 ttcaaaagcg aacgcggcta tctggaacaa ctgggcgggc agattggcgt tgcggcactt
    19261 tctgcacagc ttgacgcccg ccagccggtt gcggccctga cctctcttca gaccgacacc
    19321 gtgaccgtgc cggataaaaa gactgccacc gctgtgctgt cggctgaaga tatcgcggcc
    19381 gccaaactgc tgggtaaaac cgaagccgag ttcctgaaaa tgaaagagga aatgcaataa
    19441 tgcctacccc gattacaccg gcgatgatca ccgccctgat gacgggctac cgttctgatt
    19501 ttcaggccgg gatgtccatg gccccgtcgc agtacaaaaa aattgcgatg accgtaccgt
    19561 ccacgtcgaa atccaacacc tacggctggc tggggcagtt cccgcagttc cgtgagtgga
    19621 tcggctcccg cgtcatcgag aagatgaagg cctatggcta tgccatcgtg aacaaaacct
    19681 ttgaaggcac cgtcgccatt aaccgcgatg acttcgaaga cgataacctc ggtatttatt
    19741 ccccgttgtt ccaggagatg gggcgcgctg ctgcggcaca accggatgaa ctggtctttg
    19801 ctgctcttcg tgacgggatt aatgcggcct gctatgacgg ccagaacttc tttgataccg
    19861 aacatcctgt ttaccccaaa gtggatggct ccggggatgc gcagatggtc agcaatatgt
    19921 ttgtggcaaa aaccggctct gtcggtgcac aagcggatta cagcggtcct gcctggtatc
    19981 tgctcgactg ttcacgcgca atcaaaccgc tgatttatca ggatcgccgt aaggctgaac
    20041 tggttgccca gaccaaagtc gatgaaggtc gcgcattcac cgataacgaa tttgtgtttg
    20101 gtgcatccgc ccgtcgcaat gtgggctacg gcttctggca gatggcctac atgatgcagt
    20161 caccgctgac gctggatgca ctgtggcacg gctggtcagc catgcgcgaa tttaccgctg
    20221 acggtggccg caaactcggc atcaaaccca cccatattgt tgtccccacc tcgctggaaa
    20281 aacaggcggt gcagctgctg gagcgtgaac tgttcgcaga cggaaacgca accgtctcta
    20341 acgagatgaa gggcaagctg gagctggttg tcgcggatta tctgtaaacg gtgagccggg
    20401 cttacggccc ggcccctgcg gagggcaaat atgaatgaac atcacactgt ggcagcaaca
    20461 gatgattcag gtcttcaggt cagtggtgac aattctgtca cagtgctggc agaagtgcgt
    20521 tgcagtcgtt cggcgtttcg tcgtgcgggg ttcctgttca cgcgtggacg tcagcaggtt
    20581 gaggtctccc cggagcagct tgcccgactg gaggcggagc catgcctcac tgtgcgaata
    20641 ctacagacgc ctgctgatga tgcggggagc gtggcgggtg tggttcatgc agtggccggt
    20701 acagatttag ccgaagccgg acaggatact ccccggaaaa agaccggtaa caaagcgaaa
    20761 caggcgaaaa aggcccgcgc atgaattacg ccactgaaac cgatatgcgg gcgcgttacc
    20821 gcgaggattt gctcaggccg ttactggctg tgccccgttc ggatgagccg gacacgcgca
    20881 agctgaaccg ggcgctgaca gatgcgtcag cccttatcga cagctatctg tccgcccgtt
    20941 acacgttgcc gctggaggtt attccggccg ttcttgttca gcactgttgc gcgattgcct
    21001 tttattacct gtgcgatcag cgagcctccg atcaggcgcg tgaccgttac cgtgaagcgc
    21061 tggcctggct gaaagatgtc atgaacggca atgtgccggt cggtgtggat acgaacggtg
    21121 cggcccctga atccggggat ttaccacagg ttcagtctga tgcagcggta ttcgggcgca
    21181 accagaaggg cttcatatga ttacggaaac cgaacaggca tacatcgccc gtatccgtga
    21241 gtatttcggg aatgaactgg tgtctgttga cacgcatccc ggcgactgga gcgacagcgt
    21301 actgcgcacc atgttgatta acgccccggc gatctacgtt gcctggctgg gtgccggtga
    21361 aggccgtacc cgtggtcgcc tggtcagtca ctgggtgttc tacgtcatcg gcgacatgct
    21421 caacgggcgt gaggccagcc gtcccggact gtatcagatt gtggcccggc tgattgccgt
    21481 gcttaacggc ttcagaacag aaaaaacctc accgctttac tttgaaaagg cggtcaacgg
    21541 ttacaccgaa acccaggcgg acagtggtgc ggtgatgtat gcgctttatt tctcctgcga
    21601 ggaaatgatc gcaccgctga ccgataccag ctcgctggac gacttcctgc gtcattacga
    21661 aacctttgtg gagccgcccg gtacgccgcc gtttgaggca catattgatt tgccggggaa
    21721 cacgacaacg gcggtcgaac cgccggagga actgtaaatg aaaaccatct ttatcaaacc
    21781 cgcgccggga cgcctgattc gtgatcccga cacaatgcgc ccgctggcac aggaggggga
    21841 ggaaaagccc tttacgccgt tctggtgtcg gcgtcttgat gacggcgatg tcattcaggc
    21901 tgaaaaagcc gctgaagagg ctccggctgt atctgcggat gcaacgactg cgacagaaaa
    21961 accggtcgcc caaccggcat ccgataagga gaaaccgcaa tgattaattt tgacgggatc
    22021 ggtaatgacc gccgtatacc gctgattgag gttgagttta ataactccat ggctgtcacc
    22081 ggcactccgg cacagcgtca gtgtgtgctg ctgtttggtc aggcgagaat gaaagagaat
    22141 gccgtggacg gtgccggtgt tctggatgtt ccggtcagga tcacccgtgc gtctcaggca
    22201 acagaactct gggggcgcgg ttccatgatt gccctgatgg tggcggaatt tatcgccatc
    22261 aatcctgatg ctgaactgta tgccattgct caggggaacg gcaccggaca ggcaacggct
    22321 gcggccatga atctgaccgg aacggtcacc cgtgacggga ttgtttatgc ctgcgtgggg
    22381 gggcggcgtt atacactccc ggcaccaaaa ggtaagaaag gcaaagaact gaccgacgag
    22441 ctggccgcgc tgatcaatgc cgatcctgac gcgcccttca cggcctcctc tggtgcagga
    22501 agtggtgata atggcgcagg tctgaaaggg tctctgggga tcaccgcccg ctttaccggt
    22561 gaatgctccg ttcatgatgt gcgcctgaac tatcacgacg gggaagcaac cccggaagga
    22621 atacaggtcg ctatagccta tccgaaacaa aaagcggcta acccggatat cacccgaagc
    22681 gtggcgggga tgggcgatcg ccagtacaac tatgtcgtca tgccctataa ggatgatgca
    22741 aacctgaaaa tcatcagtga tgagcttctg aaacgctggg gaccggccaa aatgtctgac
    22801 ggcgtgctgt ggctggcaca taccggcacg tttggtgagg tgcaggcatt cggggcaaaa
    22861 cgcaatgatt ttctggcgac ctgcacatcc attcccaaag cccctgaacc ggattacctc
    22921 tgggcagcgg cggtctgtgc cacctgtgca ccctctctgg gagcagatcc tgcgcgtccg
    22981 cttcagacgc tggcgcttcc gtcacgcatg gccccggctc cggccgatcg tctgacgcgt
    23041 gaagagcgta acagtctgct gtggggaggg attgccacgg tcagtgtggc tgccggtgat
    23101 gtggttcaga tcgaacgtca ggtgaccatg tatcgccaga acgtgtacgg cgaaagtgat
    23161 cccagctatc tggatgtgga aaccatctac accctctcct ggctgcgtta ttccctgcgt
    23221 acttttatca cacaacgttt tccgcgccat aaactggctg atgacgggac gcccgttcgt
    23281 gccggtcaga atatcgtgac gccggagatc atgaagcttc agttaatttc gctgggtgaa
    23341 gagtgggtgg aactcgggct ggtcgaaaat ctggacacct ttaaaaagaa tctgcttgtg
    23401 gagcgtaatg cgtcagatcg aaaccgtctt gacgtgttat gcacgccgga tctggttaac
    23461 cagttccgct tcctggcagc acagatccgt ttcattttgt gaggtaaggc atgagcggaa
    23521 aacagtatca gggcacggcc actatccgtg tgaacggtca ggaatacgag acgcttgagg
    23581 gggctacatt ttctccgtca ggttttgaac gtgaagtggt gaaaggcgcg aaagtctatg
    23641 gttaccgcca gaaaccacgt gaagcgacgc tggactgcaa atttccggca ggcggagaag
    23701 gctcaccagc cgctgatgaa atcaacaact ggactgcggt cactatcgag tttgtggcgg
    23761 ataccggcga agtccacatg atgacgaagg cctggagcag tgaaccggcc tcgcttgacg
    23821 gtggcgggga gatctccgtg aagttcgcca gcgccaccag tacccgtgtt cagtgatcag
    23881 gaaaaataaa atgaccacac gtaagaaaaa aacggcagtt tccgaggcag ccgtgatgga
    23941 ggcaatccgg gaagcacttg agggggctga tccacgcact gccgggctga cagagcaact
    24001 ggcaaaggga tatgtggatc tgcttgatgg tctgccgttt ggtgagaccc gtgaatatcg
    24061 tgtcacgttc cgggaactga cagcgaaaga cagtattgac gcagaagcag aagccgagcg
    24121 cgtggtggag acaaacaatg gcccgatgct gatagcgtct ccgtcgttac gcggtgttgc
    24181 actgctgcgc cgtcagattg ccgcagtggg agacattgaa ggtccgttgt caccccgtca
    24241 gattggacag ttaagcgagc gcgatctctc ccgcctgatg gcagcggtca gcctgctgga
    24301 taccgcgctt gccggaaagc tggcagctga ccgggggcga tcaggcgcag tgtcgggatc
    24361 agattgaaga agcggcaatt atcctgggga tggtgacaaa aagcggtccg gaatgggcgc
    24421 ttaacctccc cctgtcgcaa ctttaccggc actgccgaca gacagaaaaa atcatcagaa
    24481 cgaagcagta agtatggccc gaaatctcag agcatctctg atagttgatt tgctcggcaa
    24541 tatctcagcc aaatcccgcc agtggtcaca ggagctgggg gcattctcac gctccggcca
    24601 ggcggggctg ggtggcctgg gaaatgctgc ccgccgtgcc gggcaggaaa cagatgtact
    24661 cggaagcagg atgcagcgca cgctggccgg ggtgcggggc agtatccgcc atgtcacttc
    24721 tgattttgac cgtcttcagg gcagtattac cggcaccatc gggcggatca gcaatcttta
    24781 cggaatgctg gccggtggtg ctgctgtata tggctttaac cggggattta ttcgcccggc
    24841 agcggagatg gaaacctata tgattcgcct gaactccctt tacaaagggg atcgggcgaa
    24901 aactgatgat gtccgcaggt gggcaataca gaatgcgaag gaaaccacct gggggctggc
    24961 tggcgtcatg caggaatata cgtccagtct gggctttggt atgagtgaca gggaggcccg
    25021 aaattttgtc accatgcttc aggatcaggg tgccgtgggc ggctggtcac tgtccgatgc
    25081 acagggggca tccctgcaac tgaaacagat gtatgcaaga ggcagtattc aggcggcgga
    25141 tgcaaatatt cttgccacct acggcattaa tgcctaccgc gtgctggctg atcgtctggg
    25201 tgtggaccag aaagtggtca gaaaacttgg tgaaaaaggg ctgcttgggc cggacagtat
    25261 tcgcctgtta tttcagacgc tggcggaaca ggccagggga gcacaaaagg acgcaatgaa
    25321 ttcctggagt ggcctgacgg ccatgatggg tgacgtctgg gaccagtttg cccgggatgt
    25381 gatggacagt gggccatttg aaaaactgaa gggtaatctc aggggatttc tttcatgggt
    25441 tgacagtgcc aaatctgatg gcagttacca ttccctggcg gaaagcacgg catcagccat
    25501 gaaccagggc tttgagtatg cccgtgatgc agtgacaggg ttttatcagg cgatcagaaa
    25561 ggtacgcgac acgcttcagg ctctgcggga tgcgggctat ggtgatgcgc tggatcgcat
    25621 tggtcagggg gcacagaccg ccgcgaaata cctgctgtac atgtatctcg ccacccgtgc
    25681 cctgaaaatg gcgcgggctg tcggaacggg gattatacgt ccgggtgctg cattgctggg
    25741 atacggatta tcagcggcgg ccttcctgac ctcacctttc cggcgctctc ctccggccgg
    25801 aacaccacca ggcacatctc agggacgagg gcaacgtttt ataaactttc ttaccggggt
    25861 aaatccggca gccgttcagc cagtacttgt aacaaactgg cccacaggag gactgtccgg
    25921 caccggcgcg accgatacat cctcaggcgg acgtcagggg cggggccgca ggaaacgcgg
    25981 accggggcgc ggaaggccgg tcacgcctcc cctgccaccg tccccagtgc caccgtctcc
    26041 ctct
//
LOCUS       DAZKJR010000069        26028 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKJR010000069 DAZKJR010000000
VERSION     DAZKJR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.069"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHOWS_002843"
     CDS             complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHOWS_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFQ0048391.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHOWS_002844"
     CDS             complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHOWS_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFQ0048392.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHOWS_002845"
     CDS             complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHOWS_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFQ0048393.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHOWS_002846"
     CDS             complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHOWS_002846"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFQ0048394.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7414..8193
                     /gene="elyC"
                     /locus_tag="ACHOWS_002847"
     CDS             7414..8193
                     /gene="elyC"
                     /locus_tag="ACHOWS_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFQ0048395.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKSSSGEPRQE"
     gene            complement(8170..9063)
                     /locus_tag="ACHOWS_002848"
     CDS             complement(8170..9063)
                     /locus_tag="ACHOWS_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFQ0048396.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDTSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHOWS_002849"
     CDS             complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHOWS_002849"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFQ0048397.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHOWS_002850"
     CDS             complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHOWS_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFQ0048398.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHOWS_002851"
     CDS             complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHOWS_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFQ0048399.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLGDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHOWS_002852"
     CDS             complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHOWS_002852"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFQ0048400.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     NAGQTLVMTEKDAVKCRAFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHOWS_002853"
     CDS             complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHOWS_002853"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFQ0048401.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14231..16495)
                     /locus_tag="ACHOWS_002854"
     CDS             complement(14231..16495)
                     /locus_tag="ACHOWS_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFQ0048402.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVAHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSQLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHOWS_002855"
     CDS             complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHOWS_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFQ0048403.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHOWS_002856"
     CDS             complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHOWS_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0048404.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHOWS_002857"
     CDS             complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHOWS_002857"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0048405.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHOWS_002858"
     CDS             complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHOWS_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFQ0048406.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHOWS_002859"
     CDS             complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHOWS_002859"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0048407.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQDNYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNMMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRIAMCFS
                     LVALSDTPVTILDPKCTAKTFPNYFEQLARISQAA"
     gene            complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHOWS_002860"
     CDS             complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHOWS_002860"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFQ0048408.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSNFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23360..24052)
                     /locus_tag="ACHOWS_002861"
     CDS             complement(23360..24052)
                     /locus_tag="ACHOWS_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0048409.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFIILALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESVDYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHOWS_002862"
     CDS             24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHOWS_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFQ0048410.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELAGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         6310 a         7087 c         6327 g         6304 t
ORIGIN      
        1 aaaaggcggc attgctgccg ccttcatttt aactcgcttg gtaaggcgct tcgtcacttc
       61 ctggaagcgt ttcagggagt tgcggcgcaa atcctcgcag gccgacgacg tgaacgtgtt
      121 cggtattctg gaagacttta cgcaccagtt tataggtggt gcctttttcc ggactgatat
      181 tttccggcgc tgcgatgatg agctgcattt gcaaacgttc acacaattca aacagcgtgg
      241 cgatagaacg agcatccagt cgcgctgctt catcgaggaa cagcaggcgg caaggagaga
      301 tatctttacc gcgcaggcgg cgagattcat cttcccagct ttgtaccacc atcaccagaa
      361 tcgacatacc ggtaccaatc gcctcaccgg tcgacagagc gccagattct gcgcgcagcc
      421 agccatcaga accacggtta acctcaactt ccatttccag atagttgcgg taatccagca
      481 gttcttcgcc aatggtctgc ggcgtgcgct gccccatatc aatctgcgga ttaagacgct
      541 gatacaattt cgccagcgct tccgagaagg tcagacggtt gctgttaaac agatcctgat
      601 gctgctcgtg ctgttcagag agcacatcca gcagcatggc gtgcgtttca cgcacgttca
      661 cattcagacg tacgctgttt acctgaccga acgatacgtt ctgcaaaccc tggttgagca
      721 tacggatacg gttctgttcg cgctgaatgg ttttgcgaat aatgttcgcc acgctgcggg
      781 aactgatcgc cagtttctgt tcacgggagg ttaactcttc ggtcagacgg ctaagttcga
      841 tctccatctg ttcgatagct tccaccggat catcggtacg aataatatcc tgacgaatac
      901 gttcacgcag atgctgataa accgccacga agaactgaat tttacgttcc ggacgttttg
      961 gatcttccga catccgcagc acgtcgcgca gatgttcgtt atccgccacc gccagacgca
     1021 gcgcacctaa cgccttatcc gacatggaac gcaaatcatc agcggagaga taagccagct
     1081 cacgacggtg taagcggcgt tcaacgccgt tatctttcac catgcgcatg actgcacacc
     1141 agcccgcttt cgcggtcact acctgctcgc gcatctcaaa gtaatcacgc tccagcttgc
     1201 gcagtttgcg ggtcaggttg tccatctccg cttcgcagaa ggtaagcgct ttttccagtt
     1261 gattgcggcg tgaacggtta ttgctcagtt gcgcgtgcaa ctcgtcacgg cgaatacgcg
     1321 cccgctcttc tgccccgcta tcagcacgca cgccgatatc ctgcaattca cgttgcagat
     1381 cgttgagtag ctcttttttg gtgtcgtaag aacttttcag cgaagccagc acctggttgt
     1441 actgactcaa ctgcgctgcg tgtccgcgca acgcttcacg cgcgcgggta cgctccgctt
     1501 ccgcctgttc cagacgttca cgcagttttt cgttgagatc gctgttaccg ctcagcattt
     1561 ctgccgagtc agagtagcta aagtgcgcac gacgctgcac cacttccgtc agggcaaacg
     1621 cctgctgacg ggcatcgcgc tgcatctgct gagagtacgc gtaatcttct tttaactgtt
     1681 cgaactgttc cgggtcgctc tgcaataccg aaacgatcgg ttccagtttc gccagttgat
     1741 tgccaaactg ctgaacaaaa cgcgcggctt cctgggcttc atccagacgt tcgcggattt
     1801 catcgacgcg atccgccagg ctgtcatcag ccaacaggtt gagacgcggc agaatgcggt
     1861 tcagcgccgt aacgccctct ttcgcctgct caaattgaat acgctgctgc tggttatcat
     1921 tttcatgatt actcagcgcc cgctccagtt cgacgcgacg gctgttcagt tgacggattt
     1981 cggcttccgg atcagactca aacgccaccg ccagatgact acctataaag cggctgaacg
     2041 cctgatgcag acgctgggtt ttctgtacat caaaagagag cgtggcgaag cgttcggaaa
     2101 gcacttcacg ctcggcatgg aggctttcaa tacggctttc acgcgcagca cgaccaaaca
     2161 gcggaacttc cggaaaacgt gagtaacgcc actgacgatc ggcgattttc accactaccg
     2221 ctttttccag ctcatcaacg ctgaacacgc tgtcatcgaa tgactgcgga tccccttcga
     2281 tcagatagag atcttccggg caatcggtca agccttccag gtgttcggtt acctgtgaca
     2341 gatctggcac cacgatggcg tggcgtgacg ggccatacag tgccgagaag tacggcgcat
     2401 cttccagact cacgtcgtca taaatttcgg acagcagcac gccgccaaaa cgctccgcca
     2461 gcgcattcaa acgctggtct tcagaaccac ctggctggct taaacgttcg atctcttcat
     2521 cgacggcgtt tttgcgcgcg cctacttcat cgcgttcaac aatggcttcg cgctcacgct
     2581 ccagcaactg ttgcagatat tcagtgacgt cctggctgga ggtgaactct tcgccgcact
     2641 gttcgctcaa ctggttgaga ctgttttgcg ctgccagcca aaccggtgca cgctgcatca
     2701 aactctgaat gcgagactgc agctgttcct gttcctggcg cagtgccatc cgctcttctc
     2761 gggcgttaga gacgctatcg gaaagagagg caatgcgtgc ttccagctcc tgatgcagag
     2821 cttccagttc atcaatatca aaattcttgc cctgacgttt gcagaaatct gccagcagac
     2881 gctcagcttc ttgctgctca cgcagacgct gttccagttc gcttaagcgc atccgcaacg
     2941 gctgaacctg ctctgccagg tgacgctgat cgaccccttc gcgcaatagt tcgcgagcga
     3001 catcccacgc ctcgttacgc gccagcgggc cgttgattgc caccaccagc tgataagcct
     3061 gctcaaactg gctgtgcgcg gtttgcgcca tgctcatttt ctgctcaaga gagagcattt
     3121 tttcggtcgc ttccagctct ttcgcctgga aggtttccag ccattcggca gcgctgtcgg
     3181 cggttaagtc cggcagatgg cacagttctt tggcacgatt aagcgcagca atcgcctggt
     3241 tatactggat cgcgcgcgtt tgctggacgt ccagcgcctg ctggtagtca gcaagctggc
     3301 ttttcagctc gtccacttcc agttcggcag cttccgcacg agcctcgttc tcttcctggc
     3361 gttcgatggc ttctgccacc acttcgtttt gctcttccag acggatctgc agctcatcga
     3421 gatccgcttc gtagcgttcg atcttctcct gctgacgcag tgcggtttgc accaggttca
     3481 ggtgatcgct ggccgcctga taatccgctt ccagatcgcc ttcggcaccg ttgtgctcag
     3541 ccagctctcg cgccatgtcg acgtgtttgt actgctcagc cgccagttgc tgacgcgaag
     3601 tatgtagctc gcgacgaaac tccagggcct tatcgagatg aacacggcgc tcgttggcgt
     3661 gacgcatgta gtccgccgcc acgtagttgg tggcttcgct gatcagatgc ttaaacaggt
     3721 cacggtctga ctgggtgaca cgaatcgctt ccagcgtcat gcggttttca cgcagcgccg
     3781 cttccatgtc ctggaacgct ttccgcaccc cactgttttc cggcaacagg tagtcgcgca
     3841 gagaacgagt aatggcgctg gagatcccgc catacagcga ggcttcgatc agacgataga
     3901 atttactacg gtcagacgct gagcgcagac gacgcgcgat gatgcccaga tcgaacatca
     3961 gcgagtggta atcggtaatg gagttgaact gtttaaactg cacgccttcc atcgcctcga
     4021 gcttgtcttt cagctcgtta agcggcagca cgcgcgcctg gcgttcgttc agagtttcgg
     4081 tcaccagttg tgtcggctgc accgacatcg gcaggccctg aatggcaaat ggtttgatat
     4141 ccactttacg atcgcgtccg gcaacctgtt gcagacgcac accgaccact acacgctggt
     4201 ggcgcgagtt aatggtgtcg agcatcgaat aacagacacc cgctttcagc ttaccgtgca
     4261 gacctttatc gcgcgaaccg ctggtggccc cggcttccgt agtgttacgg aaatgcagca
     4321 gggtcaggtc ggggataagc gccgtaacga acgctgccat ggtggtggat ttacccgccc
     4381 cgttaccgcc agaaagcgtc gtgaccagct cgtcaaggtc aaaagttcgg gcaaaaaagc
     4441 cgttccagtt aatcagcgtc agtgagcgaa atttaccgcg ttcaatcatt attcttcctc
     4501 tccgctatct ggctgactct cttcggtttc atcgttgagt tgcagatgat tttcaatcgg
     4561 cattgcttcg ccatcacgaa tcaggcgacg ctgtgcttca cggggatcgt cgccagcacg
     4621 cacatcggct ccgaagcgga acaccgattc ggtaatgcgg aacttgctgc tgtcgtggcc
     4681 cataaaccac accataccca aacgacgcag acggttgagc gaagaacgta ctttctcctg
     4741 caacttctgc cggtcaacgt ctgaaccggt tgaacggttg ttcaccagtt tcagcagttt
     4801 cgcttcatcg gccagggtga gcagttcgtc gtacagttcc tgctgggtga aaatcccctc
     4861 attcgccagc cgttccgggc tgagatagag ataacagagg attttcccga ccatcatatc
     4921 cagttccgac aagacggaac gagggatcag cgtggtggaa cgtgggcgta gatagaagaa
     4981 cccttccggt gcacgaataa gctcaacgtt ataacgcgcg taaaactctt ccagatattc
     5041 ctgaaaatcc atcaggaatg catgattatc cagttcgtcg aggccaatat gacggcctga
     5101 acgtaaggcg ctatccagcg ccggaaataa cggattcgcc agcgcctgcg ccagcttaac
     5161 cggcatcact tgttcaatat ttgtcgatga catgcgcctg taccttggct ccgtaatcat
     5221 taatcggctg ccatttcgct ggcagtccgg tgaaatctgc ttgcgctacg ccaagacgta
     5281 ccgcctgatc aataacaatg cgcgcaacgt caaagtgacg tgcacgcgga tactgtgaca
     5341 gatattcgcg taccaccaga ccaagatcca acggcacttg tctggttttg tacacggcaa
     5401 gttgttcttc gatgatcgcc gccagctgtt cgcggatctc gttaaactct tcgtactcca
     5461 gatcctcagg aagttcccca gtcacttctt catcgcgcag taccatctct tcgtcacgca
     5521 tatccagcag acgatcggca ttggcataag ttagcgccca cggctcatca aaataggttt
     5581 gtaccgactg acgtaaccgc tgagcaaaga cgcggttttt atccatatcg atcgcagtac
     5641 ggataaattt gtgtacgtgg cggtcgtagc caatccacaa gtctatggat tgctggcccc
     5701 aactgataat acggtcgagt ttgctctgca gatcgaacac cagacgatcg acaaaatgca
     5761 ggtcgtcatg ggtcatcgtc gcatcctgaa tgcgcaacag attagcctgc aatttatcgc
     5821 ctgccgcttc cagcgtatcc tgcaattcac gcagcgttcc ggaagtttct gaaagcaaca
     5881 attcacagct ggaaatcgcc gcccgccagt ctttgttcaa caactgggcg atatcatcct
     5941 tcacctgctg ctgctgttcg tccatcagac gttgcgtcag gtcgatactg tcgaaaattt
     6001 ctgctaccga atatttcagt ggcgcataaa cattacggtg ccagtgaaat tcatcaccgc
     6061 cctcttcggc ggcatctgcc gcgcgtttga gctcacccgc cacaatcgac aactgcatag
     6121 aaaggcgcag cgtagaaaac tcgcgctgac ggatgtaata gtcagtaatg ccgatgccga
     6181 gcggcgtcag acggtaaatt gcgttccctt ccgcctgctc gctggtaaag cggttcagca
     6241 gacgttgacg caccatgtcg ttgatcgcgt tgttggcgcg cacgccgatg gtttcgctgg
     6301 tttgctcaaa cgcatcactc acatggcgga atgcatccac cagctcgcct tcactcatct
     6361 caccatccag acgctcgccg ttcagcgtgg caaccgccag cagaaaagag agtcggtcta
     6421 ccggcagcga gatggagaag tcattttttc tggcccaggc aaccagttcg gggactgtct
     6481 gggaaaattc actcatactt tatccttgct ctgcggtttg cgcgcggtga catgaatata
     6541 acgccccagg gtaatatacg gttcctgacg gcaataacgc gtttctaatt caagtaatgc
     6601 ttcatagcag tcgcgctgct ggtgcttctc gcgcagataa tcatgaaaca cgcgaacgcc
     6661 tgtcttaccc ataatttgcc aaccagcttc ttccagccac agataaacct gcgccgggtc
     6721 gcgtggataa tctggcgaaa gcgtccgttt tttctttttc ggcattcccg cctgcacgta
     6781 atcaaaattc ccggcgacca tgttatgcat cagcaagccg tgcgcattgt agaacattaa
     6841 cgacaacaca ccgcctggac gcaacactga ccacagggtc tgtaatacgc tgcggggatc
     6901 ggccacccac tcgagcaccg catggaacaa tatcagatca acgggcgttt ccaaatgtga
     6961 agcaacatcc tgagcggcgc aatgtataaa ttgcatgttg tcgctcacac cttttgcttc
     7021 tgccgcctgt tttgcgcggt cgatcatctg cgcagaaaga tcgcataaaa tgacctgatg
     7081 cccgcgctca gccattttga ttgcggtctg cccttctcca ccgccagcat ccagcacacg
     7141 cagtttttgc gggcccattt ccgccagcac gcgatcgaga tcctgccaca gaatagcctg
     7201 tcgaagctgc cctttggtgg tgccgtaaat gttacgggaa aacttttccg caatatcatc
     7261 aaaattgcga tcctgcattg ctttctccac cggttggcta agacgttttc tgataaagac
     7321 gctattttgt cacagccacg gcgagaatga acccgtctgg tgtaatatcg ctggcgatcc
     7381 cttgctatat ggttaaaaaa ggaaccagaa aggatgcttt ttacactgaa aaaagtgatt
     7441 ggcaatatgt tgctgccact tccgctaatg ttgttgatta ttggcgctgg cctggcgctg
     7501 ctgtggttta gccgttttca aaaaaccggg aagattttta ttagcattgg gtggctggcg
     7561 ctcttgttat tgagtttgca accggtagcc gaccgcctgc tgcgtcccat cgaaagcacc
     7621 tatccgacat ggaataattc gcaaaaagtg gactatatcg tggtgctcgg aggtggttat
     7681 acctggaacc cgcagtgggc accaagctcg aatttaatca ataacagtct gccccgcctg
     7741 aatgaaggta ttcgcttatg gcgcgaaaat ccgggatcaa aactgatctt cacgggaggc
     7801 gtagcaaaaa ccaatacggt gagtacagcc gaagtaggtg cccgagttgc gcaatcgctg
     7861 ggcgtgccgc gcgagcaaat tatcactctg gatttgccaa aagataccga agaagaagct
     7921 gcggcagtga aacaggcgat tggtgacgcc cctttcctgc tggtgacatc cgcgtctcat
     7981 ttgccgcggg cgatgatctt ttttcagcag gaagggttaa atccgctgcc agccccggcg
     8041 aatcagttgg ccatcgactc gccgcttaac ccgtgggaac gggcaatccc ttctccggtc
     8101 tggttgatgc atagcgatcg tgttggctat gaaacgcttg gccgtatctg gcagtggcta
     8161 aaaagttcgt caggcgagcc acggcaagag tgattttgat gccagatcga agttgcgccg
     8221 actaaatcgt ccggtattaa ccagttgcgc aacttcatcc cataacacat acaaccaacg
     8281 ccgccagatg aacgactccg ccactggcgc gcgttgcagg taactccaga gcaaatcttc
     8341 cgccagagaa gtatccatta gtcggaacag ttcgtattcc cgcggtgccc aaagcattag
     8401 tcccggcccg accatcgcca gtaactgatc gctgcgcgaa tctttcagca tgctgcgtaa
     8461 acagaagtta ccgtgaatca gcacacaatt gtcgttaaag ccttcaaata atgccgggag
     8521 acattcgcga gtacgaaaca ggatccgctt atcctgcatc gttagaccgg tgttattgaa
     8581 ctgattgagc gtggtccata gcacttcaac atgttgccgg taccatgagg gccagaaatt
     8641 ttcctgagta ttgtcgaccg cgccgacgca accgcgactg tcctgacggt gccaggccag
     8701 taaggcttca acgatttgat ctttgagttg ttcccagcgt tctggtgttc gggctggtgc
     8761 ctccaccgaa acaccacgca tccgctccag tagcaggaca tcggggccgg ggtgttcttc
     8821 atgcgtcatc acaccataaa tagtcggcat acggacagtc ccacttcttg ccagcatggt
     8881 ggttttccat gccagttgtc gggcttttcc gggcgtacta aagcttcttg ccattaacgg
     8941 cattgggttt ccctggctgt catacaaagc ccacaacgcc gtatccgctt tttcattgac
     9001 gcactcaata cgactgagtt tttcgcccag taaatggctt aattcggcac gcagctgttc
     9061 cattcagatt accctcataa aagctactgc tttcataatg agcgccaata taaaagatgt
     9121 cacctgaatg agatcaattg ttgaaaagaa aaattgagga aaatcgtaac ggtacgacac
     9181 tcctcccaaa attggctgga gtgtcgtgaa gtgaaattag cgcatttcag cgcgaacgcg
     9241 ctcgagatct tcaggggtat ccacacctgt gccaggaact tcctgggcga cagcaacatg
     9301 gattttttcg ccgtaccaca gaacacgaag ctgctctaac atttcgatgt gttctaacgg
     9361 acttggctgc cagttgacgt aacgacggat aaagcctgca cggtagccat aaataccaag
     9421 atgacgcagg aagttatcgc caacggtttc aaggcctttt gcaaaacgat cacgatccca
     9481 aggaatggtg gcgcgagaga agtacagtgc atacccttca gcgtcgagga ccactttcac
     9541 cgcattcggg ttaaacgctt cctccgcatt gtggattggc accgccagag tcgccatacc
     9601 cacctgacgc tgagcgaggt tatcagcaac ctgacgaatg attgtcgcag ggatcatcgg
     9661 ttcatcaccc tgcacattaa cgatcacagt gtcgtcgctg aatgcgcatt tttcgacaac
     9721 ttccgccaat cgttctgtac ctgactgatg atcggcgcgc gtcatacata cttcgccgcc
     9781 agcggcttca acagcgcggg caacatcctc atgatcggtt gccacgatga tgcgctcggc
     9841 acctgattcg cgcgcgcgtt caagaacatg aacaatcatg ggtttgccgt taatatcaac
     9901 caatggttta ccgggcagac gcgtggacgc gtaacgcgcg ggaataatga ccacaaaact
     9961 catgatttac tctcatcagc agtcagcacg cgggcttccg tttccagcaa cactggaatg
    10021 ccatcgcgca gcgggaaagc caggttgtcc agtttgcaaa taagctcttg tttttcctgg
    10081 ttataccaaa gttttccgtt gcaaaccggg caggcaatga tttcaagcag acgatgatcc
    10141 atagttcctc cagatggatc gggttatgaa tgcataaatc ttatcataat catttatgcg
    10201 acggggtcta tttcccagcc ggcgcggcaa tcagtaaaga ggccttgcgg gcagcgtcct
    10261 aatgtcaccc gcgttgcctg ctgccagtta gcgaaatcag taatcgcctg acgtaaccct
    10321 ttttgcagcg ttgtcgttgg tttaatgcct tcttgtaacc acagagagat aacttcaagg
    10381 atgcctgtct ggcgatgcat tttggcatcc attcgcccaa ctaattgccc acgatgtaat
    10441 aacggcagaa caaaatagcc atactggcgt ttcggcgctg gggtatagca ctccagccgg
    10501 tagctaaaat caaaaagctg ctctgcgcgt ttgcgatccc agacaacagg atcaaaaggc
    10561 gaaagtaccg cgctgtgcgt tgcagtgagc tttccggcca gcgcacgctc gagtagcggc
    10621 agcaaatcag catgcagcca aagattgccc aatttttcaa catgcacagc aatgatttgc
    10681 tgctgttcag ccctcgcttc acgccacgcc gccagtgccg gacgttttag ccggtagtaa
    10741 tctgccagcc actgttcgcg aaatataccc aggctacgcg cactgttatc cagcatgatg
    10801 atttctgctt ctgcttgcga aacgagatcg cgctcatcat tccactcagg catgacacgg
    10861 tgagttaaat cataaacgcg ctggaagttg cgccgttcaa tcaccatcac ctttccggca
    10921 gtaaataaac cttccagatg tcgtttatgc ggcttccact cccaccagcc gctcgcccct
    10981 ttacgaggat gctcaaaatc ggctgaacgt actggcccct tatcatgaat atgctgaatt
    11041 aactgtgcaa tttccgccgc atgttcctgc atccaggcgt ctttatattt ccagcccatt
    11101 ttttcaggtg ccagcatgcg gtggcgaata agacgaaaat cgctacgcgg cataaagcag
    11161 gcttcatgcg cccagtattc cattaattcg ccacgcgcca gagactcatc cagccactgg
    11221 gcaggataat ttcccagacg actgaaaagc accagatatg gactacgggc aacaatattg
    11281 atggtatcga tttgcagcaa ggacatgcgg gagatcgttg ccggaatatc ccccaacgac
    11341 gctcgacggc ggggtttgtt taacaggcct tgcgcggcga ggtgaagatt acgcgcatca
    11401 gcaagagaga ggtgcggcag cgacattcat gactccatca atcgaacgct gccgcggcgt
    11461 aactagttgc cagaagccag cgaggttagt tgcgcaagca gtttcgctgg ttcatcacct
    11521 gaaagctgtg cgtcaacagg caaataccac caatttcctt cggcaaaggc ccggcatttc
    11581 accgcatctt tttcagtcat taccagcgtt tgcccggcgt ttaccaacgc gctgacatcc
    11641 gcatggttca aagactgatg atcggccagc ggtacacatt tttccggttg tacgccgcac
    11701 atcttcagcg tggcaaaaaa gcgcggcgga tgtccaatcc ccgccatcgc cactacatgt
    11761 tcaagctgag caacgtcaca acgcgtaccg gtacgtaaat tcaccgcctg acccggcagc
    11821 agatgcatgg ggatttcacc gctgcggggg acaccgccgt tgacgattac cgcatcaacc
    11881 gactttaagc gccccgctcg ctcacgcatt ggccccgccg gcaaccacca gccattgcca
    11941 aagcgacgca caccatcaat aacgacaatt tccacatcac gcgccagacg ataatgctgt
    12001 aaaccatcgt cggttacgat gatctgcaca tcagggtgtt gcgccagaat ggcttttacc
    12061 gcgtcagaac gcacgggaga aaccgcaaca ggcgcgccag tgcgttgata aatcaacaca
    12121 ggttcatcac ccgcctgtgc tgtggtggta tctgccgaca ataacagcgg ataagattca
    12181 gccttaccac catatccccg cgatacaacc cccacgcgaa taccgcgctg ttgcagctgt
    12241 tccaccagcc agacaacgac cggggttttt ccgttgccgc ctgcggtgag attaccaacc
    12301 acgacaaccg gtacgggggc acgccaggcg cgcttcagtt ttagtttata gcaaagacgg
    12361 atcgcgccac tcaccaggcc atacaaccag gagagtggca gcaatagccg ccataaaggg
    12421 gattcaccag accagatttt ttcgatcatt ggccaaactg cattttgtga agttgcgcgt
    12481 aaacgccacg gtgctcaagc aaatcgttat gcgtaccgcg ttccacaatg acaccatcct
    12541 cgacaaccac gatttcgtct gccttttcaa tggtagacaa gcggtgggca atcaccagag
    12601 aggtacggtt tttctgcaac tcatccagtg ccgcctgaat cgcacgttcg gattcggtat
    12661 ccaacgccga ggtagcttcg tccagaatca gtatcgggct atcacgcaac aaggctcgag
    12721 cgatagcaat acgctgacgc tgaccgccag agagcagcac gccgttttca ccaatcactg
    12781 tatcgagacc gttatccatc ttattgatga agtccatggc gtaggccata cgcgccgctt
    12841 cttcaatttg ctcacggctg tactgttcag tccgtgcgta agcaatgttg ttagcaaccg
    12901 tatcgttaaa cagatggaca ttctgcgaca ccagagcaac ctggttacgt aacgacgcca
    12961 gggtatactc gcgcagatcg tgaccatcca tcaggatttc gccttcatca atatcgtaaa
    13021 aacgcgtgat caggctggcg atggttgatt tacctgaacc agagcgtcca accagagcaa
    13081 ccgtcttccc tgccggaatt ttcaggttga tgttacgcaa tgcgggcaca tcacgtcccg
    13141 gataagtaaa ggtgacattg cggaattcca cgtcgccagt cgcacgctcg atcacgcgct
    13201 taccttcgtc tttctcctgc tcactgtcca gaatggtaaa cagcgtctga caagccgcca
    13261 taccgcgctg gaactgggcg ttaacgttgg tcagcgattt cagcggacgc ataagtgcaa
    13321 tcattgaaga gaaaacaacg gtaatcgtac cggcagtcag gctatccatg acacttggga
    13381 agcttgccgc atacagaaca aacgccagcg ccaaagaggc gatcagctga atgatcggat
    13441 cagagatgga agaggctgaa accattttca tcccctgaag acgcattcgg ttgctgactt
    13501 tatcgaagcg tttcgtttcc acttcctgac caccgaaaat caatacttct ttatggcctt
    13561 tcagcatttg ttcggcgctg gtggtcacct gccccatggt gttctgcatg tttttactga
    13621 tgttgcgaaa acgcttcgat acaacgcgaa tcgcaatcga aacaatcggt gccagcacaa
    13681 tcaaaatgat cgacagttgc caactgtaat agaacatcat gatgaacagg ccgatgatcg
    13741 acgcaccttc acgtacaaca gtaatcagtg cgccggaaga agaagaagcg acctgttcgg
    13801 aatcgtaggt gatacgtgac aacagcgtac ccgttgactg tttgtcaaag aatgaaaccg
    13861 gcattcccat catgtgacca aacaggcggc gacgcatagt cattaccacc tttcctgata
    13921 cccaggagat acagtagctg gagatatagc tggtgatacc acgtaaaatc atcagcccga
    13981 tcaccaccag cggcatccac atcagcacgg agcgatctgt tttaccaaag ccatcatcaa
    14041 gaagtggctt aaggagcgat aacatgaagg tatcgctggc tgcgttgagg attaacgcta
    14101 cgcccgccac gatcagaccc gctttaaaag gcgcaatggt tggccacagt cggcggaatg
    14161 tctgccacgt agagagatct ttgtcgttat gcattcaaaa aaccagcatt tgttgaaata
    14221 gccgcatatt ctacccgtta tccactggca cgccaaacca ctgatgatac caacgaggta
    14281 aaatttgctc tctgaggctg ctaatccgcc agccttgcgc tgaaaaattg accgttattt
    14341 gaccctgatg tggagtatca agccacttat aaccctgttg ttgatagcga tgtttaactt
    14401 tgcttgaggg cagtcgccat gcgttatagc gcgatgccga tgcgagtgcc acttttccat
    14461 tcactcgctg aattaatggc aacgatgatg aggtattact gccatggtga ggtacctgaa
    14521 gtaatgttgc ctgcatttgc tgccagtaac ggcttagcat cttttgttca gcgggggctt
    14581 caatatcacc ggttagaaga atgctattcg tcccgtcatc aatcttaacc acacaggaat
    14641 ggttatttcc tttatcgttg ctaccttgta aaggccagtg cgcgctgaaa cgcaatcctt
    14701 gccattgcca cgcttcgcca cgcacacagg gctgatgatg ttcccagttt aactgacttc
    14761 ggatccataa catcggccat gtgtgcaata ttgagtccag ccctccccgg tgatccagat
    14821 gttcatgact caaaataacg ccttccggtt caagattatg ccagtggagc caggggataa
    14881 tcagttgttg cccactatcc ccttcgggcc aggccagtcc cgtgtcatag agaatcgctt
    14941 tgccgtttcg cgcaatgacc attgccagcc cttgcccgac atcaagcatg tacacttgcc
    15001 actcgtcagg tcgaggtttt tgccacagcg gcaagcacat cagcaagcct acagccacac
    15061 acattgctgg caacgttcgc caggcgttta atcgccatac caccagtaag aaccatgggg
    15121 aaaatgttag ccattgccaa cgttcagcaa tgttgatcca cccttccggc aaactcttta
    15181 acccccagaa aagtaaagcc aaagaccggt cggcaagaaa ccataaccct tgttccagaa
    15241 taaacggccc gcttaaatgc gcaaccatcg cggcgaggat caacggaacc gtgataaatg
    15301 tcaccagggg aattgcaaat agatttgcaa taaacgaggt cagactaatg ccatgaaata
    15361 tgacgatttg cacgggcata agcaggagtg tgattcccag ttgtaaatga atgagggaaa
    15421 caactgcacg caataccggc ggcagttgcc actccggaca gggaaaccac tggtaccaaa
    15481 aaatcagtgc cgcgaccgca gcggcagaga gccataaact ttgcgagaga atggcaacag
    15541 gatccatcag caaaattgcc gccagacaac atatccatac atcccagcca ctccattgtc
    15601 gcccacttaa cttaagcatt ccccacatgg caagcgccac cacggtacgc aatgcaggag
    15661 gttgcatccc agtcagccag gcataaaaag cagcacagca gattccgcca attaatggca
    15721 tttgccagtg gatccagcgc ccaggcagaa aaacttgccc accgcgaatg agtccggcag
    15781 ccagcaacgc cgcaaaagcg atatgcaatc ccgatatcgc cattaaatgc gccgttccag
    15841 tatcgcgcat gatatttttg atttctttgg gaacggataa ccgttccccc atacctaaac
    15901 caagaataac cgcgttccac atatagggtt gcagcgttgt ttgtagtgac gccagatact
    15961 gtgcacgcag gctacaattc ggttcaatga cacttgcctg cagaaagcgg ccggtgagcg
    16021 gctgatgctg ggcaatggca taacgctggc tatcaaagcc gccatcatta agttgaccat
    16081 gaactgcacg aactttgagt ttcattgacc acagttgtcc agcacaaacc gcttgcggaa
    16141 gatattcgcc atacagcacg aggcctggcg cagggaatat acgtcgacct tgtagatgag
    16201 taatttgacc gtaatgagtg gtcataccgt cagtagcagt gatctcaaca attgcatcct
    16261 gcgtcgcgcc agttaaggtt tctcctgccc acaaaatttg ctttgctgct aatatgcccc
    16321 aaacgaaaaa cagcaacgtc agcgcgatat aacggacagt tttaaaaggg ataaatacca
    16381 gtacgcaggc gaagacggtc agagacgcaa gggttaatgt cccaggcaat tggggcaaaa
    16441 tcagcaacgg aaaaattccg caaattatgc atacgccgac tgtcgttatt ttcatgcacc
    16501 cctccctgtt tatggaaagt gtgcaacttt gttgagcggt tgtctggtgg gagttttgcg
    16561 ttccccgagg cacgttcaga agaatttaac gagagggtga aaactgttca aaactctgcg
    16621 aggctgcttc cagttccaga ttagagaaat gaaaaaagca cccgacaggt gcttttctcg
    16681 cgttcaagtt tgagtaaaaa cttaaccgta aatattggcg cgatcgcgca gttctttacc
    16741 tggtttaaag tgaggaacgt attttccttc cagttctact ttatcgccag tcttcggatt
    16801 acgtccggta cgtggtgcgc ggtagtgcaa agagaaactg ccgaaaccgc ggatttcaat
    16861 acgctcgccc tgcgcaagag tcgaggccat atgctccagc atctctttta ctgcatcttc
    16921 aaccgtcttg gcgggaatgt gcgattgctg ggtggcaagt ctttctatca attctgactt
    16981 ggtcatgatt cctccggttc cttaaaggca aatttagcgg ctgctgttgc tgcattgatt
    17041 aagggcggcc gtagccgccc ttagtgcttg attacaggac gaaacctgca atctgtcaag
    17101 taaactcaac aaacttcgga ataaaaatcc cgaagagtca gagaattact cgcctttagc
    17161 tgctttgaaa gcttcagcca ttgcgttgtt ggagaagttt gcatcttcct gtttgttaac
    17221 agttgcgatt gcatctttct cgtcagcttc gtctttcgca cgaacagaca ggctgattgc
    17281 gcggttttta cgatcaacgc cggtgaattt agcttcaact tcgtcgccaa cgctcagaac
    17341 cagggtagcg tcttcaacgc ggtcacggga tgcttcagaa gcacgcaggt aaccttcaac
    17401 gccgtcagcc agttctacgg ttgcgccttt agcgtcaact gcagttactt taccggttac
    17461 gatagcgcct ttcttgttca gagcaaccca gttgttgaac ggatcttctg cgagctgttt
    17521 aacgcccagg gagatacgtt cacgttctgc gtcaacctgc agaacaactg cagcgatttc
    17581 gtcgcctttt ttgtattcac gaactgcttc ttcgcctgca acgttccagg agatgtcaga
    17641 caggtgaacc aggccgtcga tgccgccgtc caggccgatg aagataccga agtcagtgat
    17701 agacttgatt ttaccttcaa cacggtcgcc cttgttgtgg gtttccgcga actgctgcca
    17761 cgggttagct ttgcactgtt tcagacccag ggagatacga cgacgttctt cgtcgatatc
    17821 cagaaccata acttccacta catcgccaac gttaacaact ttggacgggt ggatgttttt
    17881 gttggtccaa tccatttcgg aaacgtgtac caggccttca acgccttctt cgatttcaac
    17941 gaagcagccg tagtcggtca ggttggtcac gcgaccagtc agtttggtac cttccggata
    18001 acgtttagcg atagctaccc acggatcttc gcccagctgt ttcaggccca gggatacacg
    18061 ggtacgttcg cggtcgaact tcagcacttt aacagtgatt tcgtcgccca cgttgacgat
    18121 ttcgctcgga tgcttaacgc gtttccaggc catgtcagtg atgtgcagca ggccgtcaac
    18181 gccgcccaga tcaacgaatg caccgtagtc agtgaggttc ttaacgatac ctttaacttc
    18241 catgccttcc tgcaggtttt ccagcagctg atcgcgctct gcgctgtttt cggattcgat
    18301 aacggcacga cgagaaacaa caacgttgtt gcgcttctga tccagcttga ttactttgaa
    18361 ttcaagctct ttgccttcca ggtgcagagt gtcacgcacc ggacgaacgt ctaccagaga
    18421 acctggcagg aacgcacgaa taccgttcag ctcaacagtg aagccgccct taactttgcc
    18481 gttgataaca ccggtaacag tttcagcatc ttcgtaagct ttttccagcg tgatccaggc
    18541 ttcgtgacgt ttagctttct cacgggacag cagagtttca ccgaagccgt cttctactgc
    18601 gtccagagca acgtcaactt cgtcacctac ctggatttcc agctcgccct gggcgttttt
    18661 gaactgctca gccgggatgg cggactcaga tttcagacca gcgtcaacca gtactacgtc
    18721 tttgtcgata gcaacaacaa cgccacgaac gatagaaccc gggcgggttt cgatttcttt
    18781 taaggactct tcaaagagtt gagcaaaaga ttcagtcatg tttaatcttc aggtttatat
    18841 ttaacgtcca cctggctcca tgccggatgg ggttgtttaa catacccgct gtcattccat
    18901 tgcaacgggg gtactgcaaa ttcggtcgct tatgcgagag ccaatttctg gcgcgcgtat
    18961 tgtagcgctt tttcaatcac ttgctcaatg cttaaggtgg tggaatccaa cactaaagca
    19021 tcggctgccg gaaccagtgg cgctaccgct cggttacgat cgcggtcgtc gcgttctttg
    19081 atctcagcca aaaggcgctc aaagttaaca ctaaagccct tctcctgcaa ctgtagcatg
    19141 cggcgatgcg cacgttcttc cgaggaggcg tcaaggaaaa ttttcactgg cgcatcaggg
    19201 aataccaccg ttcccatgtc acggccatcg gcaatcaggc ctggtaattc gcgaaacgcg
    19261 cgttggcgac gcaataatgc ttcacgaacg cgtgggaatg ccgcgacttg tgaagctgca
    19321 ttcgccactt cctgagtacg aatttcgcca ctgacatctt ccccttcgag gatcacttcc
    19381 agattgccat tggtcgacac aaaacgtaca tccagatggg atgccagcgg caccagcgca
    19441 tcttccgatg caacatcaac atggtgatgt aatgccgcca gtgccagtac gcgataaatt
    19501 gcacccgagt ccagcagatg ccattgcaac gcttccgcca tagccttaca caaggtgcct
    19561 ttccctgcac cgcttgggcc atcaatggta ataaccgggg caattgccgt catctttatc
    19621 tccttaaaac aggcgtaccg ttaacataac cgcgcgcatt atacgcgccg acgccacaaa
    19681 tcgttaacgt tgtgtgcaaa aaccagtttt gtctgtattc agatggggat ttattgcgca
    19741 attatagcgg gctggcagaa gaccagcccg aaagatgaca atgatttact taccagaggc
    19801 gatacgatca cggatgtgtt gcgcacgctc tgttgatggt gggtgagaat caaacatgga
    19861 ctgggtacga ccgccatcca ggctggccaa tttttcaaag ctgccaacca gcccctgggt
    19921 gctgatgcca cgtttcttca acagatcaaa ggagaaatca tctgcttcgg actctttatc
    19981 gcgggagtat ttagcattga tcgcgccttc tgcgatatca cctaattgtg agcgagaaag
    20041 ctgggaagcc acaccgctgg tagcggaaat cgcatcacgt gcggcaacga tcgcataaga
    20101 agctttcatt tcagccagcg agtgacccaa cgcgacgtgg cccagttcat gacccagaac
    20161 gccttcaatt tcgttgtcgt tcatcatgtc catcaggcca ctgtagacac gaacacaacc
    20221 gttcgccatc gcccatgcgt tgacgtcgct ggtcatataa accttatagt tgaccggcgt
    20281 gccattaata ttgttaccca atgctttggc gatttttgcc agacgtttcc cgtacttgct
    20341 ggatttgctt gcgacttgat tgccgctgtc catttcggca cagccctgat tggcaatcgc
    20401 ttttgcatca gcatcgctta acgttgctgc tttataggca tttaaacctg agctgatagc
    20461 catattggta tcaataccgt gggtattttg acaccctgtc agtaatgctg cagaggccgc
    20521 aatcgccagc agtaatttag tattcttcat gtgaatcatc cgttgattaa tcatatataa
    20581 atattatgta tcaataactt acattgacaa gcgacgctac cattaatcaa aatgactcac
    20641 aaggtccgaa aaaaaacgcc ggcaatgtgc cgacgttttg ataaagaaag atttggctat
    20701 ttattgcacg ttgttcattc aggctgcctg gctaatccgc gccagctgct cgaaataatt
    20761 cggaaatgtt ttggccgtgc atttgggatc aagaatcgtc actggtgtat ctgacaacgc
    20821 caccagcgag aaacacatcg ctatccggtg atcattgtat gtcgcgatct cggcaaagtt
    20881 cagcttttcc ggtggagtga tacgaatgta atcgtgcccc tcttctactt ccgcaccaac
    20941 tttacgcagt tctgttgcca tcgcaaacag gcgatcggtc tctttaacac gccagttata
    21001 gatattgcgc agcgtggtgg tgccttttgc aaataacgcc gccgtggcaa tggtcatcgc
    21061 cgcatcggga atatggttca tatccatatc aatagcgttc agttcacccc gcgtgcagga
    21121 aatataatca tcgccccagc aaatggtcgc gcccattttt tccagcacat cagcaaagcg
    21181 aatatcaccc tgcatactgt tgcgtccaat gccggtcact tttacagtgc cgcctttgat
    21241 tgctgctgct gccaggaagt aagaagccga agatgcatcg ccttcgacca aataagtacc
    21301 cggagactga taagactgcc cgccttttac gacaaattgt tgatagtgct gattttcaat
    21361 ttcaacacca aacgtcttca tcatattgag tgtgatgtcg atataaggtt tagaaaccag
    21421 atcgccttta atacgaatca ccgtatcttc cggtgcaaga ggcgcagtca ttaatagtgc
    21481 ggtgaggaac tggctggaaa cggagccatc aacgtcaacg ttgccgccag taaagccgcc
    21541 ctgtaaacgc aatggcggat aattgtcttg ttccaggtaa gtgatcttcg ccccgcccag
    21601 gcgcagcgca tccaccagat gaccaatcgg gcgctctttc atacgcggct caccagtcag
    21661 cacaatatca ttgctaccca gacaaagagc tgccgccagc gggcgcattg ccgttccggc
    21721 gtttccgagg aacaactcca gggcaccttc tgcgtgtaat ggaccgccgt taccgataat
    21781 ttcgcaacgc gtacgatcgg ctgaaagcgt atagcttacc cctaacgctg ttaatgcatt
    21841 cagcatatgg cgcacgtcat cgctatccag cagattggtt aatactgttt tgccgtgtgc
    21901 taatgccgcc agcaataaag cgcggttaga aacgctcttg gaaccaggca gattaatagt
    21961 gccatcgaca cgagcgatgg gttgtaacgt cagggattcc atgaactcaa ctctctacaa
    22021 cagaaataaa aaccccacag gctggctgtg gggattaagc aaaatttcgg cattagccgt
    22081 gacggcgttc gaactcaacc atgaagtctg tcagggcttt aacgccttcc agcggcatgg
    22141 cgttataaat agacgcgcgc attccgccga ccacacggtg acctttcagc gcatgaaggc
    22201 cagcagcaaa agactcttca aggaacaatt tgtcaagcgc actgtccgcc aactggaacg
    22261 gcacgttcat ccgcgaacgg ttagctttcg ccacgtcatt gcggtagaaa ttgctgttat
    22321 caatcacccc atacagcagt tctgcttttt gctgattgat tttatccatt gcagctacac
    22381 cgccgttcgc tttcagccat ttaaagacca gaccagacag ataccaggca aatgtcggcg
    22441 gcgtgttaaa catggagtcg ttatcgttga gaatggaata atccagaatc gacggacacg
    22501 cgatattcgc tttgcccagc aaatcttcac gaacgatgac gattgtcagg ccagccgggc
    22561 cgatattttt ctgcgcgcca gcgtaaatca cgccataacg gctgacgtca atcggacggg
    22621 aaagaatggt tgaagagaag tcggcggcga ccaccacatc tttgccgaag tctggcgttt
    22681 cgtcgatagc gataccgtcg atggtttcat tcgggcagta atgcatataa gcagcattat
    22741 cagagagttg ccattcgcgc attggcttaa ccgcgcgcag accatcaaca gtcactttgg
    22801 cgtcaaagac attaggcgtg cagtattttt tcgcttcttt aatggcactt gccgcccagt
    22861 aaccggcatc aacataatct gcggtggttt tatcaccgag aatattcagc ggaacagcag
    22921 caaactgacc gcgaccgccg ccatggcaga acaatacctt gtagttggag gggacattaa
    22981 gaagatcgcg aaaatccttc tcggcttcct ctgcaacctg aatgaactct ttgccacggt
    23041 gactcacttc catcaccgac gtaccaagac cgttccagtc gcgcagttcc tgttgagcct
    23101 gtttaagcac ctctgccggt agcattgccg gaccagaact aaaattgaag atttgagcca
    23161 tttcccctca ccacgttgcg ttgctattaa cccgcggtca atcgattgcg accacaggta
    23221 atacattatt cctgtggcta tcggttttat cattcagtga cacacatcgc aatgagtaaa
    23281 accgttgagg gcaaaaatgt ggcttgcatc acacaaaaga atctctcgga ttattagcaa
    23341 aaagccgatg tttttactgt cacgtcaccc gttttgcccg acttgccttc gtccattctg
    23401 gatttgcaca gcgcggacat aattgttttt cccccgcgtt catatgaatg atttcactgc
    23461 aacgaatgca ggcatagtcc accgactcgg gtaccacttt gtaacgctga gtacagtcac
    23521 tgggtaaaat aagtaagccc ggtttcacct tgtcatcttc aaagaaatag acactgattt
    23581 gcccgttggt ttcgagaatc gccagacgta cttgccccaa ctgctctaca ccgcgcaatc
    23641 gtagctccat aaagaactca aattccgtca tgttggagtt attgagtttc gaccaggcca
    23701 gctcgccatc ttcaataatg acaaccggct tgccttccag aagatcttcc agtttttcac
    23761 tgtgcgccat caaccacatt accaggcggt ataacaacgc cagaataata aagacgataa
    23821 gtaccgggac catcggcaca tcatcataaa acgccacatc tcccgccgct gatcccagcg
    23881 tcagaatgat taacacttca aaaagcgaca tctgccgcac accgcgtctt ccggtcattt
    23941 tgaggaacaa aaaaaccagt acaaaggtat aaagactacg tagtgccact tcgccaagga
    24001 aatcaaaagg cactttatca aatgccatac ggtggagatc gaacgccttc atttatatgc
    24061 tcttatcgtg gtttatccgc taaaagcata gtcaacagat catcggcatg gcggcggtga
    24121 tagcgccttt gagataaaac ccgtattatt gcgcgctttc cgtacgacta aagtgatttt
    24181 catgacgcaa acatttatcc ccggcaaaga tgccgcactg gaagattcca tcgctcgctt
    24241 ccagcaaaaa ctttcagacc tcggctttca gattgaagag gcctcctggc tgaatccggt
    24301 gcctaacgtc tggtctgtac atattcgcga caaagagtgc gcactgtgtt ttaccaacgg
    24361 taaaggcgca accaaaaaag cggcgctggc ttctgcactc ggtgaatatt tcgagcgtct
    24421 ctcaaccaac tacttttttg ctgatttctg gctgggcgaa accatcgcca acggtccgtt
    24481 cgtgcattat cccaacgaaa aatggttccc actgagcgaa aatgacgatg tgccagaagg
    24541 gctgctcgat gaccgtctgc gcgcatttta cgatccggag aatgaactgg ccggcagtat
    24601 gctgattgac ctgcaatccg gtaacgaaga tcgtggtatt tgcggtctgc cgtttacgcg
    24661 tcagtctgat aatcagaccg tttatattcc gatgaatatc attggtaacc tgtatgtttc
    24721 taacggtatg tccgctggca atacccgtaa cgaagcacgc gttcaggggt tgtccgaagt
    24781 tttcgaacgc tacgtgaaaa accgcattat tgctgaaagc atcagcttgc cggaaatccc
    24841 ggcagacgtg ctggcgcgtt acccagcggt ggttgaagcc atcgaaacgc tggaagcaga
    24901 aggtttccca atctttgctt atgatggttc gcttggcggc cagtatccgg tgatttgcgt
    24961 ggtactgttc aatcctgcta acggtacctg ctttgcctct ttcggtgcgc atcctgattt
    25021 tggcgtagca ctggaacgta ccgtgaccga gctgctgcaa ggtcgtggcc tgaaagattt
    25081 ggatgtgttt actccgccaa ccttcgatga tgaagaagtt gctgaacata ccaacctcga
    25141 aacgcacttt atcgattcca gcggtttaat ctcctgggac ctgttcaagc aggatgccga
    25201 ttatccgttt gtggactgga atttctccgg cactacggaa gaagagtttg ctacgctgat
    25261 ggctatcttc aacaaagaag ataaagaagt ttatattgcc gattacgagc atctgggcgt
    25321 ttatgcttgc cgaattatcg tgcctggcat gtccgatatt tatccggctg aagatctgtg
    25381 gctggcgaat aacagtatgg gcagccattt acgtgaaacg attctttcgt taccaggcag
    25441 cgagtgggaa aaagaagatt acctgaacct catcgagcaa ctggatgaag aaggttttga
    25501 tgactttacc cgcgtgcgtg agctgttggg tctggcgacc gggtcggata acggttggta
    25561 caccctgcgt atcggtgaat taaaagccat gctggcgctg gctggtggcg atctggaaca
    25621 ggctctggtc tggaccgaat ggacgatgga gtttaactca tcggtattca gcccggaacg
    25681 cgccaactat tatcgctgcc tgcaaacgtt gttattactg gcgcaggaag aagatcgcca
    25741 gccgctgcaa tatctgaatg cgtttgttcg catgtacggc gcagatgccg tggaagccgc
    25801 cagtgcggca atgagcggcg aagcggcgtt ttatggtttg caaccagtag atagcgatct
    25861 gcacgcgttt gctgcacatc agtcgttgtt gaaggcctac gaaaagctgc agcgcgccaa
    25921 agcagcattc tgggcaaaat aaaatcaaat agcctacgca atgtaggctt aatgattcgt
    25981 ctgagttata ttacggggcg tttttttaat gccccgcttt acatatat
//
LOCUS       DAZKJR010000070        25050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKJR010000070 DAZKJR010000000
VERSION     DAZKJR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.070"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..374)
                     /locus_tag="ACHOWS_002863"
     CDS             complement(<1..374)
                     /locus_tag="ACHOWS_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048411.1"
                     /translation="MTGLSEQAKELHMMMGRRKPPKIQAVLNTRYWKNTEKTIKRTVG
                     MNIHTLSQWCGEQYEKMFISKVAEVYMLHHTAAILRRKGVYVDVFKIPSKIMNPPEAR
                     ANCTGCPSSQNVSPFFAYSEPNT"
     gene            488..1372
                     /locus_tag="ACHOWS_002864"
     CDS             488..1372
                     /locus_tag="ACHOWS_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFQ0048412.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICTTHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRHQELSLATVTVRVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDHLF"
     gene            1491..2168
                     /locus_tag="ACHOWS_002865"
     CDS             1491..2168
                     /locus_tag="ACHOWS_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048413.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            2174..2412
                     /locus_tag="ACHOWS_002866"
                     /pseudo
     CDS             2174..2412
                     /locus_tag="ACHOWS_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            2501..3319
                     /locus_tag="ACHOWS_002867"
     CDS             2501..3319
                     /locus_tag="ACHOWS_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFQ0048414.1"
                     /translation="MRLASRFGYANQIRRDRPLAREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGNEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            3658..4131
                     /locus_tag="ACHOWS_002868"
     CDS             3658..4131
                     /locus_tag="ACHOWS_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFQ0048415.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            4147..4623
                     /locus_tag="ACHOWS_002869"
     CDS             4147..4623
                     /locus_tag="ACHOWS_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFQ0048416.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            4692..4913
                     /locus_tag="ACHOWS_002870"
     CDS             4692..4913
                     /locus_tag="ACHOWS_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFQ0048417.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            5076..5444
                     /locus_tag="ACHOWS_002871"
     CDS             5076..5444
                     /locus_tag="ACHOWS_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFQ0048418.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            5534..5911
                     /locus_tag="ACHOWS_002872"
     CDS             5534..5911
                     /locus_tag="ACHOWS_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFQ0048419.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTSPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKR"
     gene            5908..6396
                     /locus_tag="ACHOWS_002873"
     CDS             5908..6396
                     /locus_tag="ACHOWS_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFQ0048420.1"
                     /translation="MKLSLTLEADSINVRALNMGRIVVDVDGVELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            6416..6613
                     /locus_tag="ACHOWS_002874"
     CDS             6416..6613
                     /locus_tag="ACHOWS_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFQ0048421.1"
                     /translation="MESLTTETALDILIAWLQNNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            6698..7564
                     /locus_tag="ACHOWS_002875"
     CDS             6698..7564
                     /locus_tag="ACHOWS_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFQ0048422.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMQSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHRSKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISGMQADRLADLERMLHLLSGKPIPDNR
                     GNITINLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            7636..7899
                     /locus_tag="ACHOWS_002876"
     CDS             7636..7899
                     /locus_tag="ACHOWS_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFQ0048423.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            7896..8222
                     /locus_tag="ACHOWS_002877"
     CDS             7896..8222
                     /locus_tag="ACHOWS_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0048424.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(8636..9172)
                     /gene="yghD"
                     /locus_tag="ACHOWS_002878"
     CDS             complement(8636..9172)
                     /gene="yghD"
                     /locus_tag="ACHOWS_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFQ0048425.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(9174..10352)
                     /gene="gspL"
                     /locus_tag="ACHOWS_002879"
     CDS             complement(9174..10352)
                     /gene="gspL"
                     /locus_tag="ACHOWS_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFQ0048426.1"
                     /translation="MSSMLEIFIPLCASDPIRWQHRTTDVEHGIWSDVDNEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGPDAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(10349..11326)
                     /gene="gspK"
                     /locus_tag="ACHOWS_002880"
     CDS             complement(10349..11326)
                     /gene="gspK"
                     /locus_tag="ACHOWS_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFQ0048427.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVAQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(11323..11928)
                     /gene="gspJ"
                     /locus_tag="ACHOWS_002881"
     CDS             complement(11323..11928)
                     /gene="gspJ"
                     /locus_tag="ACHOWS_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFQ0048428.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(11925..12296)
                     /gene="gspI"
                     /locus_tag="ACHOWS_002882"
     CDS             complement(11925..12296)
                     /gene="gspI"
                     /locus_tag="ACHOWS_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFQ0048429.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(12293..12856)
                     /gene="gspH"
                     /locus_tag="ACHOWS_002883"
     CDS             complement(12293..12856)
                     /gene="gspH"
                     /locus_tag="ACHOWS_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFQ0048430.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(12860..13315)
                     /gene="gspG"
                     /locus_tag="ACHOWS_002884"
     CDS             complement(12860..13315)
                     /gene="gspG"
                     /locus_tag="ACHOWS_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFQ0048431.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(13332..14555)
                     /gene="gspF"
                     /locus_tag="ACHOWS_002885"
     CDS             complement(13332..14555)
                     /gene="gspF"
                     /locus_tag="ACHOWS_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFQ0048432.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(14555..16048)
                     /gene="gspE"
                     /locus_tag="ACHOWS_002886"
     CDS             complement(14555..16048)
                     /gene="gspE"
                     /locus_tag="ACHOWS_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFQ0048433.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(16048..18108)
                     /gene="gspD"
                     /locus_tag="ACHOWS_002887"
     CDS             complement(16048..18108)
                     /gene="gspD"
                     /locus_tag="ACHOWS_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFQ0048434.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDDQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(18138..19097)
                     /gene="gspC"
                     /locus_tag="ACHOWS_002888"
     CDS             complement(18138..19097)
                     /gene="gspC"
                     /locus_tag="ACHOWS_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFQ0048435.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTVVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(19115..19525)
                     /gene="gspS2"
                     /locus_tag="ACHOWS_002889"
     CDS             complement(19115..19525)
                     /gene="gspS2"
                     /locus_tag="ACHOWS_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFQ0048436.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(19591..20400)
                     /gene="pppA"
                     /locus_tag="ACHOWS_002890"
     CDS             complement(19591..20400)
                     /gene="pppA"
                     /locus_tag="ACHOWS_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFQ0048437.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLKALF"
     gene            complement(20530..25050)
                     /gene="sslE"
                     /locus_tag="ACHOWS_002891"
                     /pseudo
     CDS             complement(20530..25050)
                     /gene="sslE"
                     /locus_tag="ACHOWS_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
BASE COUNT         5741 a         7018 c         6298 g         5993 t
ORIGIN      
        1 gtgttaggct ctgaataagc aaaaaaagga gagacattct gagaagaagg gcaaccagta
       61 caattggctc tcgcttcagg cgggttcatt atttttgagg gaatcttaaa aacatccaca
      121 taaactcctt tacgcctcaa tattgctgcc gtatggtgaa gcatatagac ttctgccact
      181 tttgaaataa acatcttctc atattgttct ccacaccact gactgagcgt atgaatattc
      241 atacctacag ttctttttat cgttttttct gtgtttttcc agtatcgggt gttaagaaca
      301 gcctgtatct tcggcggttt tctccgcccc atcatcatat gaagttcttt tgcctgctca
      361 gataatcctg tcatttatta cctccgaata tattccatta aaaatggaaa taattatacg
      421 cccatattac ctgaatgata aaccggatta ctgtgatccg gttcagtgaa atcaacagga
      481 taccggtatg tcattcagcc attcttccct ctctgcacaa gtgaaatcat atctgacgtt
      541 tcttcctgaa gaaattcgcc agaaaatcct tgaacatctc cacggtgtta ttcattacga
      601 gcccgtgatt ggcattatgg gtaaatccgg caccggcaaa agcagtctgt gtaatgccat
      661 ttttcagtcc cgtatctgca ccacgcatcc cctgaacggc tgcacccgcc aggctcatcg
      721 cctcaccctg cagattggtg aacgcagaat gacgctggtc gatctgcccg gcattggtga
      781 aacaccgcag catgatcagg aataccggga gctttaccgt cagttactgc cggaactgga
      841 tctgattatc tggatcctgc gggctgatga acgtgcgtat gctgccgata ttgccatgca
      901 tcagttttta ctgaatgaag gtgcagatcc ctcgcgtttt ctgtttgtac tcagtcatgc
      961 cgatcgcgtg tttcctgctg aagaatggaa cgacacagaa aaatgcccgt cccgtcacca
     1021 ggaactctca ctggcaactg ttacagtccg ggtggcaacc ctgttcccgt catcatttcc
     1081 ggtactcccc gttgctgcgc ctgtcggctg gaatcttccg gcgtttgtgt cactaatgat
     1141 ccacgccctg ccaccgcagg caaccagtgc ggtttattca catatccggg gggaaaaccg
     1201 ctctgaacag gcccggaaac acgcacaaca gacttttggt gaaaccatcg ggaaaagttt
     1261 tgacgtcgcc gttgcccgtt tcagttttcc ggcctggatg ttacagcttc tgcgtaaagc
     1321 ccgggaccgc attatccgcc tgctggtcac actgtgggat cacctgttct gagcgccccg
     1381 gagtcccccg tggcaaccgt atacctttac acgacaacgg attacggtaa ccactgccgt
     1441 ataccgtctg tcattatcaa taacacactc tttaatttca gaggtcagtc atgaatacca
     1501 gaattctttt tcccctgctg ttcactgtgg catccttctc cgcctccgcc ggtaactggg
     1561 ctgtcaaaaa cggctggtgc cagaccatga cggaagatgg tcaggcgctg gtaatgctga
     1621 aaaatggcac gattggtatt accggcctga tgcagggatg cccgaatggc gtacagacgc
     1681 tcctgggcag ccgtatcagt attaacggta acctgatccc cacatcacaa atgtgtaatc
     1741 agcagacggg attcagggct gttgaggtgg aaatcggaca ggcgccggaa atggtcaaaa
     1801 aagccgttca ctccatagca gagcgtgatg tgtccgtttt acaggcattt ggtgtacgaa
     1861 tggaattcac ccgcggtgat atgctgaagg tctgtccgaa atttgtcaca tcacttgccg
     1921 gtttttcccc gaaacagacg accactatta ataaagactc cgtcctgcag gctgcccggc
     1981 aggcatacgc ccgggaatat gacgaggaaa caacagaaac cgctgatttt ggctcttacg
     2041 aagtaaaagg caataaggtt gagtttgaag tattcaatcc tgaagaccgt gcgtacgaca
     2101 aagtgaccgt cacggttggt gctgacggta atgccaccag tgccagcgtt gaatttatcg
     2161 gaaaatagcc ggtatgtcgg actgtcaccc tgttttattg cccgaaggcc ctttctcacg
     2221 caaacaggca atggctgcca caacagctta ccttaccgca atgtgcttat tgaagatgac
     2281 catggaacgc atttcctgct ggttatccgc aatgccgaag ggcagctacg ctggcggtgc
     2341 tggaattttg agtctgacgc cggaaaacag cttaatgtgt ggctcgccag tgaagggctt
     2401 ctcagacaat aaaagtcttc atttcatcca tcaggccgcg tcttctccgg gagacgcgcc
     2461 ttttcattta taccgccaac tcattcataa ggagcaaatc atgcgattag caagtcgttt
     2521 tggttatgcg aaccagatac gccgtgaccg tccactggca cgcgaagaac tgatgtacca
     2581 cgtcccgagt atttttggag aagaccggca cacctcccgc agtgaacggt atgcgtacat
     2641 tcccaccatc accgtcctgg aaaatctgca gcgggaaggc tttcagccgt tcttcgcctg
     2701 ccagacccgt gtgcgcgacc agagccgccg ggaatatacc aaacatatgc tgcgtctgcg
     2761 gcgggccgga cagataaccg gtcagcatgt gcctgaaatt attctgctca actcccatga
     2821 cggttcatcc agctaccaga tgttacccgg atattttcgt gccatctgca ccaatggcct
     2881 ggtctgcggt cagtcgctgg gagaagtccg ggtgccacac cggggaaacg tggtggacag
     2941 ggtgattgaa ggggcttacg aggtggtggg cgtttttgac cggatagagg agaagcgtga
     3001 tgccatgcag tcgctgattc tgccgccacc ggcacgccag gcgctggcac aggcggcact
     3061 gacttaccgt tatggtaatg agcatcagcc cgtcaccacc gctgatattc tgacaccgcg
     3121 acgccgggag gattacggta aggacctgtg gagtgcttat cagaccatac aggagaatat
     3181 gctgaaaggc gggatttccg gccgcagtgc aaaaggaaaa cgtatccaca cccgtgccat
     3241 tcacagcatc gacaccgata ttaagctcaa ccgcgcattg tgggtaatgg cagaaacgct
     3301 gctggagagc atgcgctgat aaaacatatc cctgaaggcg tgattcaggt accagaatca
     3361 cccgacgccc cggaggcgct gccggggtgt cgttccattt ctgatgatga cctgtgcacc
     3421 tggtgtaccc gtctgcttta ccgtcccgga gaactcagtc tgtgcagact gagtacaggc
     3481 aatggcatct ggccatccgt ctgtgaccgg aacggttacg cgtacggctg tccggaattt
     3541 cagccgaata tcattcatcc ctgatattgc cgggtctcac ggtcagtgat gaacgtgatg
     3601 ttgtcagccc ccggccttta tttcctctca tttattcaca ttacaaggat ttcacacatg
     3661 aaaacattgt ctcagaacac cacctcttca gcctgtgcac cggagactgg cctgcaacaa
     3721 ctggttgcca ccatagtccc tgatgaacag cgcatcagct tctggccgca gcattttagc
     3781 ctcattccac agtgggtgac cctggagccc cgtgtcttcg gctggatgga ccgtttgtgc
     3841 gaagactact gcggtggtat ctggaatctg tacaccctga acaacggcgg cgcatttatg
     3901 gcccctgaac cggatgacga tgatgacgaa acatgggtgc tgttcaatgt catgaacggt
     3961 aaccgcgctg aaatgagtcc ggaagccgcc ggcattgcgg cctgtctgat gacgtacagt
     4021 catcacgcct gtcgtacgga gtgttatgcc atgacggtcc actattaccg gctgcgggat
     4081 tacgccctgc agcatccgga atgcagcgcc attatgcgca tcattgactg aatggaggca
     4141 gggacaatgc agcagctttc ctttctgccc ggagagatga cgcccggcga gcgcagtctc
     4201 attctgcggg ccctgaaaac cctggaccgc catcttcatg aacccggcgt ggccttcacc
     4261 tccacccgtg cggcacggga atggctgatt ctgaacatgg caggactgga gcgtgaagag
     4321 ttccgggtgc tgtatctgaa caaccagaat cagttgattg ccggtgaaac cctcttcaca
     4381 ggcaccatca accgcacaga agtccatccc cgggaggtga ttaaacgcgc cctgtaccac
     4441 aatgccgccg ccgtggtgct ggcgcacaat cacccgtccg gtgaagtcac ccccagtaag
     4501 gcagaccgcc ttatcaccga acgtctggta caggcactgg gcctggtgga tatccgggtg
     4561 ccggaccatc tgatagtcgg tggcagccag gttttctcct ttgccgaaca tggtctgctt
     4621 taacccgtca ccgccacaat cacatcacac ctgttttcac ttttatcttc tgtcttcaga
     4681 ggtatcccat tatgaaaatt atcacccgtg gtgaagccat gcgtattcac caacagcatc
     4741 ctgcatcccg tctatttccg ttctgcaccg gtaagtaccg ctggcacggc agtgctgagg
     4801 cgtataccgg tcgtgaagtg caggatattc ccggtgtgct ggccgtgttt gctgaacgcc
     4861 gtaaggacag ttttggcccg tatgtccggc tgatgagcgt cactctgaac taatgttccg
     4921 tcacccggaa aacgagtacg accagatgac ctcagacgcc gctgtggatg aggcccttct
     4981 catcaatgaa tacatcttca ccgaagccgg tctccacacc ggctgacctg tcatcgttaa
     5041 ccccttctta accgattttc tgtgaggact ttatcgtgtc agacacgcac catgagacaa
     5101 attatcccga cgactataac aaccgcccct ggtgggggct gccctgcacc ataacaccct
     5161 gttttggcgc ccgtctggtg caggagggta accggttgta ttaccttgcc gaccgcgccg
     5221 gtatcagagg ccggttcagc gacgcggatg cgtaccatct ggaccaggcc tttccgctgc
     5281 tgatgaaaca actggaactc atgctcacca gcggtgaact gaatccccgc catcagcata
     5341 ccgtcacgct gtatgccaga ggactgacct gcgaagccga caccctcggc tcctgtggtt
     5401 acgtttatat ggctgtttat ccgacgcccg aaacgaaaaa gtaaatcatc agaataacct
     5461 tctgctccgg cctggtgttt tcaccacgcc actttcccat ttttcatctc tgcatatcag
     5521 gaaaatcttc agtatgaaca cattacccga cacacacgta cgggaggcat cgcgctgccc
     5581 atctccagta accatctggc agacactgct cacccgactg ctggaccagc actacggcct
     5641 cacgctaaat gacacaccgt tctccgatga acgtgtgatt gagcagcata ttgaggcagg
     5701 catttcactg tgtgacgcgg tgaactttct cgttgaaaaa tacgcgctgg tgcgtaccga
     5761 ccagccccgg ttcagcacta gtccccgctc tcagttaata aacagtattg atatcctccg
     5821 ggctcgccgg gcgaccggcc tgatgacccg cgacaattac agaacggtaa ataacattac
     5881 ccggggtaag catccggagg cgaaacgatg aaactttccc tgacgctgga agccgacagc
     5941 attaacgtgc gggcgctgaa catggggcgc attgtcgttg acgtcgatgg tgttgagctt
     6001 gctgaactga ttaacgtggt ctgcgataac ggtcactccc ttcgtgttgt tgatgaatct
     6061 gaccgggcct caacagactg cacgccacca tttgctgccc tgaccggcat acgctgcagt
     6121 accgcgcata tcacggcaaa agacagcgcc tggctgtact cgctgtcaca tcagacgaat
     6181 gacttcggcg aatcagaatg gattcatttt accgggtccg gttatctgtt acgtactgat
     6241 gcgtggtcgt acccggttct gcgacttaaa cgcctgggcc tgtcaaaaac gtttcgtcgt
     6301 ctggtcgtaa cgctcatccg ccgttatggc gtcagtctca ttcatctgga tgccagcgct
     6361 gaatgtctgc cgggtttacc caccttcaac tggtaaccgg gacaaaagac acagtatgga
     6421 atcattaacc acagaaacgg cgctggatat tctgattgcg tggctacaga acaatatcga
     6481 ctgcgaatcc ggaattatct tcgacaacga tgaggataaa acggattcgg cagcactgct
     6541 gccctgcatt gaacaggcca gggaagatat ccgtacactg cgccaactgc agtttctgca
     6601 acagaaccgg tgagtcttac tcaccagact tcattccact gacgccagcc tgaatacggc
     6661 tggcgttttc gtttataaac caaaaggaaa aacgatgatg tcagatatca cagcctcccg
     6721 tccggaagtg gtcaccgggc atacggacgt tatttgctca accagtatcc gccacattct
     6781 ggctgtacga aagagtacac tgctgcaaat cgacacactt atccggcaac tggctgaaat
     6841 ctcagcaatg acagaaagta ttggcggtaa aaccgcactg gactgggcga tgaaacagga
     6901 ttttcgctgc ggctgctggc tgatggagaa accggaaacc gcaatgaaag ccatcacacg
     6961 caatctcgat cgcgaaatct ggcgtgacct gatgcaacgt tccgggatgc agagtctgat
     7021 ggatgcgcag acccgggaca catggtacag gtcactggaa tatgataatt ttccggaaat
     7081 cagtgaagcg aacattctga gcacatttga acaactgcac cggagtaagg atgaagtgtt
     7141 tgagcgggga gtgatcaacg tcttcaaagg gctgagctgg gattacaaaa ccaactcacc
     7201 ctgtaaattt ggcagtaaaa ttatcgtcaa caatctggtg aggtgggacc agtggggatt
     7261 tcatcttatc agtggaatgc aggcagatcg cctggctgac ctggaaagaa tgttgcatct
     7321 gctcagcggt aaaccgatcc ccgacaaccg ggggaatatc accattaatc tggatgacca
     7381 catacagtct gttcagggta aagaggacta tgaagacgag atgttcagta ttaaatactt
     7441 taagaaaggc tcagtacaca tcacgttcag gaaaccagaa ctggttgaca ggcttaatga
     7501 tattattgcc agacactacc cggaagccct ggcaagcaca gtgaacaaaa gccgcaaggc
     7561 ataaaagagg tattttacaa ttcgcgaata ttaactatta ttaattgata gcaatcaatc
     7621 ggcgagaaaa tcaccatgcc taccagtgta tccctcagtc cttactttga aacgtttatc
     7681 cgcgaacaaa tagaaagtgg tcggtataac aatacaagtg aagtcattcg tgccggactt
     7741 cgtgctctgg aagaacggga acagcagata aagcttgaat cactccagag cgccgttacc
     7801 gcaggcatca acagcggtga aagtaaaagt gctgaagagg tattcgggcg actgacgcac
     7861 aaatacaaaa agatggcaga aggtgaacag ccaatatgaa actgactgtt tcaccattag
     7921 cagaacacga tcttgaagct ataggtgact ggatcgctca ggataatcct gtccgtgcaa
     7981 tcagttttac cgaagagctt taccaacaat gtctgttgat agccgaatcg ccagtactct
     8041 acagagaaag acccgaatta ggggctggga tcagaggttg ttcgtatggt cgttaccttc
     8101 tgctgttcag ggtactggat actgaagttc gtatcgaacg tatcgttcat ggttcccggg
     8161 atatcaacag ggtacttgca gagccagacg acaaaccatc agatcatttc tggcagccat
     8221 aaacaaaatc tgtgcgttaa cgctccctaa cacaggtagc caggtatttt ataaatctct
     8281 gtattttgtt gttacttcga ttccgcaatt cagcatatcg aaccagacaa aatgattaca
     8341 gcaaagcaca aaaaagaggg attatagcgt caaaagaatt cgaattgcac atgggggcct
     8401 cattggtggc ctcaaaaaca acaaagacaa aacaatcgat acaaaacaat tggttatata
     8461 accagtttca ttccgagtcc gggcaccact attctaaaga acccgctcac aaggcgggtt
     8521 tttgcttttg gatctgacaa taaccttcac gaaacaaatt agcttataaa gcctggggga
     8581 attactctcg ccacgttaac gagagtaatt ttattgatat taatctcctg atactttatc
     8641 cccgtccaaa ctccagccgc tgcacattca ccatcccagt cttctcagca gcactgacat
     8701 caatttgtgt cacccgcagc gcatattttt catccagtgc gtttaaccat ttcagcaggt
     8761 cattaaacac cacaggttct atccagacct gaatattctc cccacgctcg gctatccgct
     8821 tgatgaccac cgagtgcgcg gaagcactgt cactgatgac ccgcgatacc tgcgcaggcg
     8881 ttgttgtggc agattttcgc gctgcaataa tatccggcgc ggcgttcttc agtcgggtgt
     8941 tcattgccac cagctgctgc aacatcgtct cctgttgttc tatccgttcg ctcaacggct
     9001 gccagatgag gacgtaatat ccgacgctaa acaggaacac caccgctgcc agtaacatgc
     9061 ccttttcacg cggagaacgc cccgccagat gttgtgtcag ccagtgttcg ccacggctta
     9121 actggcgttc acgccattgc tgaaaatagt gaataaattt atcgcgtaac atgttatttc
     9181 ctccgcaacg ttacgccgcc ggaaaccgca tcaccctctt tctgtaacgc gtcctgttgc
     9241 acaacataat ctgccgccag tgcgctacgc agtttgtcga agctggcaaa gttcgcagcc
     9301 cgtagctgga ggtgaagcgt ctggcgtttt tgatcaaagg tgaaaccacg catttcgatg
     9361 tcggtaagtg acgctgattt cagggtactg gcgatcgctg acaattctgc gagcagccgg
     9421 gtatcgtcgg cctgcgggcg atattttttc agcgccatcg tcacctgaga gcgtaaattc
     9481 acaatccgct tctgctccgg gaacagtgtt aagaactgtt tctccgcctg ggtgcggctt
     9541 tgcgccacct gttcgctgac gctccataac gtcacgcccc gttccactgc cagcgcaacc
     9601 agaatcagca atatcggcag aatcatcacc cgccagcgcg cccactgttt tcggtagctg
     9661 acgcggggct gccacggccc tgttagcagg ttcccttccg gttcgccata agtggtaatg
     9721 gcgggcagag ccgtaacggt caggcgttca gcatctgggc cagcccatgc tgatagctct
     9781 tccggtgcaa tgccgaccac ggttagtgaa agcggtaaat cctgctcatt gagctgtgcg
     9841 cggaacatga ccggagccag cgccagcccg gcgctccatc cccggcattc atcgatgcgg
     9901 cagataaccc gttgcgcatc gccagccata aacccacaag gaatggacat ccagtctggc
     9961 gcgacggtag cgcgggtgat gccgtttgcc tgcaaccact gcgcaatgtt gcgcatatgc
    10021 tgttggtgaa tcacagctac ggttgccagt tgctggtcga ttttcaacgg ggcgaaatgc
    10081 agttcatcga tatcctggtt cagctcttct tccagtaagg cgggcagaat agtcggtatc
    10141 tgcttgcggg cgacatcagg cagttcaacc tgccagacgc tgatccattc gccgggaatg
    10201 tagagtcgaa tcgcatcagt ttgcagccat tgctggagct gttcgttgtc aacgtcagac
    10261 cagatgccgt gctccacgtc ggtggtacgg tgctgccaac ggatgggatc ggaggcgcaa
    10321 agcgggataa aaatctcaag catggaactc actcactttc tcctgtctga tgccagagaa
    10381 ccgaaaagtg ttgtgggccc atgcggacaa ttaacgagtt catcgtcagt tcaatctcat
    10441 tcacggtgat atctgaacgc agccagaagt aattgctgtc cacgctcagg acggttttta
    10501 gctgtttttt agtacgctca tcgacgtcag caagtagcgg ctgtgcaaga aactgatcga
    10561 catcttccca gcccttcgcc ggacgttgtt gtaataacgc ccgcgcctga acagggctta
    10621 accacgggtc aaacagcgcc tcaagaatca cactttgcgc gacgtccagg gtattaatgt
    10681 tgatttgctg gcgggtcatc ggcagcgcac agaccagcgg tttcagtttt tgataaagtc
    10741 cggcgtccat tccctgcacc acgcgcatct cgctgatatc agccagcggt tgattagcgg
    10801 cgtaaaacgg caccgaacgg gcgagatact cgctgtcttc acggcccaga cgcgtctgca
    10861 cgctgcggtc ttcgtcaata aactcccaca ggctttcggc tatcagttcg gcccgataag
    10921 caggcacatc caggcgcgtg atcagggcaa tcagttgttg taccgcgagc ggacgcgacg
    10981 ccgtcgtcgg ctgagcgaga gcattcaggt taaagcaggc ctgtgcgtca cgcagagtga
    11041 cggcgatttg cccctgcggc agtgggaaaa aacgagggcc ggaagcccac ggctgcgcca
    11101 gatgcacgcg cttttcattt ttcaggctca gactgagtgc gctcaacgcc aggctttccg
    11161 cactggcgct gtaccacagc gcctgctggt actcctgctg gctgcgcgtt cgcccaagtt
    11221 gttgctgcat tcgcccggaa agcgtgatgg tcaccagcat cataaccgcc agcaatacca
    11281 gcaccacgac cagtgccatt ccgcgttttg gtggtgaggt gatcatgata attgcggccc
    11341 gcgtaacaac cagatgcgtt caatctcgcc ccattgcggc gaatgcagag tcatgcgcac
    11401 cgccactggt atcgcctgca ctgatgacca gctctcctgc cagcgcgtgc cgtcgtagaa
    11461 ctgcaaacgg agcgaatccg ccgggattaa tttttgcgtt gttggcttca cgctgcctgc
    11521 cgcatcggtc agtggccagg ctaaccgttc gagataacca ccatgaatgc ggtaaccgac
    11581 ggtgagcaga ttactgcgcg gcagacgcat caacggatta accacgccgc cgcgcacaaa
    11641 acgcatccct tcactctcag acgccagcac gccagcgccc gccagtaacg ctggttcacg
    11701 ctggccctga tcgcctctta ccggacgcgg catcatttgt gtcagatcgt ggttcaaaaa
    11761 actcatcgtt tgctgcatga ggtttagttt ttgatcgtgt ccggcgacgg cgctattcac
    11821 gcgtgtaacg ccgtttgtca cctgctgcgc catcagtgcc agtgaggcaa aaatggcaat
    11881 tgccaccagc atttccagta acgtgaaacc agcgcaagtc cttctcactg ttggcctccc
    11941 acggcgctaa accaggcgcg tcgtgactga atcaccgacg aaaagtcttc gtgacggctg
    12001 acttcaatat ccactgcctg gagcagcgcg ttgcctgtac tcagcggtgt ggttcgccag
    12061 taccagcggc ttcctgccat atcactctcg ccctggtgcc tgaccgctcg ttgttcacgg
    12121 gtcatcaggt aaagcagtgc ggtctggttt tcagctaccc agcccgctac cgttttttct
    12181 tcaaggattt gctgattact cagcgcgccg ctggcgatct gtaacaccgc catggcggcc
    12241 aacgcaaaaa tcgccagcgc gagcattact tccagcaagg taaatccacg cttcatggca
    12301 tcccctcatc acattgattg agggataaca cgccatcgtg cgccagcttt accgcccaac
    12361 acgcgttttg tgccgacgcg taaaaacgca gtgtaaacgg cgtggcgggt tcaaaaggcg
    12421 aaaaacggat ctgcggtgtc ttctttttcg cctctttttg cagctccagt tcaatatcgt
    12481 gcagcgtcag gcgacgacgc tgcagctcca gcgcatattc cttctgccag atatcacttc
    12541 cgggttgtaa cagcatctgc acctgtttcg gcaccgtaac ctgtgccgat aaccgggtcg
    12601 cagaaacggg caaccactgc ccgtgacgac gctgcataaa ctggtaacct ggtgggtcga
    12661 ttagcacacc gagtgttttc ccttcgatca ctgccctgtc cttaaactgc gcaaagcgag
    12721 tcagaaaagc ctgcgccgct tttttcgcag gcggctctga agcggtcgca aacgtctgta
    12781 ccacgcccgc actggcaagg ccgataagga aaatcaccag catgatttcc agaagtgtga
    12841 atccgcgttc aggcactgat tactgaaact cctgcaaatt ccagttaccg atatctgcgc
    12901 cagcgccctc cccgttttcc tgaccatctg cccccagggt atacacatca aacagtcctt
    12961 tttcacccgg gctgagatac tgataatcat tgccccacgg atcttttggc agtcgtttaa
    13021 tgtatccacc ggtacggtag ttgcgggagt ccgccatatt ggccggttgc tggatcagcg
    13081 cctccagtcc ctgttcggta gtcggataac gcccgttatc cagtcggtac atatccagcg
    13141 cattctccag cgccacgata tcgctgatgg ctttttgccg atcggctttc tctttgttgc
    13201 ccagcaggtt aggcaccacc agacttgcca ggacgccaag aataacaatc accaccatca
    13261 cttccagcag ggtaaaacct gcccgtggtt tttgtgtgcg ggataacgaa ttcatgtgat
    13321 aactccgtaa attacattcc aaccatattg ttcagttgca gcatcggttc gaggatggcg
    13381 atgacgataa acagcaccac gcccgccatc atcaccacca gcgccggctc aaacagccct
    13441 aacgccagcc ccacctgggt atcaaattct cgttcctggt taactgcggc ctgttcaagc
    13501 atggtttcca gttcaccgct ctgttcgccg gaggcgatca tgtacagcat catcggtggg
    13561 aacaggcgca actccgccag cgcagcgcgc agactgcttc cttcgcggac gcgatctgcc
    13621 gccagcagca gttgttgttc gacatagcga tttgccgaca cggcggcagc ggtctgaatg
    13681 ccttccagta gcggaacact gctggcggtg aggatgctta acgtgcggga aaaacgcgcc
    13741 gtattcagcc cgcgcgcaac acgccccgtc acgggcaaac gcagcaaggt tttatcccag
    13801 cgtaggcgca tggcaggatt tttgagtagc cgttgcccca gcaccagaag ccccagcaat
    13861 cccgccagcc agtaaacgcc gctggtctgt aacgtgtcgc tcatggcgat gagcgtgcgg
    13921 gtggaggcag gcagcgcgtg tccgagatga tcaaactgtt cgatgatttt cggcaccact
    13981 gccgtcagca aaatagtgac tacgcccgtt gccaccacca gcaataccag cggatagagc
    14041 atggcctgca acagtcgtga tttcaggcgc tgtcgctgtt cggtgtaatc cgcaaggcga
    14101 ttgagcacca cgtcgagatg cccggatttt tctccggcag cgaccatcga acaaaacagg
    14161 gaatcaaaga cgcggggatg ttcgcgcagg ctgtccgaca gggtataacc ttcctgaatc
    14221 cggctgcgca gcgccattcc gaggcttttc acatgcagtt tttcactttg ctcactgacc
    14281 gcctgtaagc aggtttccag cggcattgct gcctgcacca gcgttgccag ttggcgcgtg
    14341 aacagcgcca aatctgccgc cgccacgcga cgatgtgcgt gccgccgacg ctgcaacatt
    14401 ccccctgtcg aagcattcag ccgggcttca atgtgcacgg ggataagctc tttaccacgc
    14461 aacaactggc gggcatgacg cgcggaatcc gcctcaatca ttcctttggt tttgcgacca
    14521 ttacgctcca gcgcctgata gtaaaacagt gccattacgc ctccatagtt acccgcagaa
    14581 cttcatcgag agaggtttct ccggcgagca ctttctcaat gccgttgctg cggatgcccg
    14641 cagagtgttg ccggacataa cgttccagct ccagttcccc ggcctgacgg tggatcaaat
    14701 cacgcaatgt ggcgtccacc acgatcaact catggatggc agtacgcccg cggaaacctt
    14761 tgtgattaca ggcggggcag ccctgcggat ggtacagcgt gacagtacgg gcgtcggtaa
    14821 tccccagcag gcgtttttct tcgtcagtgg caggcgcggg ctggcggcag tcggggcaca
    14881 gcgtgcggac cagtcgctgc gccatcacgc ccgtcaggct ggaagagagc aggaaaggct
    14941 caacgcccat atcctgcaga cgtgtgatcg cccccaccgc cgtgttggta tgcagcgtgg
    15001 aaagtaccag gtgccccgtg agcgacgcct gaacagcgat ttctgcggtt tctgtatcgc
    15061 ggatttcacc gaccatcacc acatccgggt cctgacgcaa aatcgcgcgc aggccacggg
    15121 caaaggtcat gccgacgcgg gtgttgacct gcgtctgacc gatcccttca atcatgtatt
    15181 cgatggggtc ttcaaccgtg agaatgttgc gcgaatggtt gttcagctcc tgcaatccgg
    15241 cgtacagcgt ggtgcttttg ccggaacccg tcggtcccgt caccagaaag atgccgtgcg
    15301 gtttgtgtaa cagctggcgc aactgcacgg tcagttgctg actcaacccc agacgttcca
    15361 gcgtcaggcg ggcctggttt ttgtccagca gtcgcagcac cacgcgctcg ccccaggcgg
    15421 agggcatggt ggagacacgc acgtcaatcg cccgaccgcc cagcagcagc gcaatacgcc
    15481 catcctgcgg tacgcgcttt tcggcaatat ccagccgcgc catcaccttg atacgcgata
    15541 ccagcagcga ggccagtttg cgccccgggc gcagcatttc atgtaatgtg ccgtcaacac
    15601 gaaaacggat caccagactc ttttcaaatg tctcgatgtg gatatccgaa gcgccttctt
    15661 taatcgcctc tgccagcatg gcgttgatca gtttgatgat tggcgcgtcg tcgtcgcttt
    15721 ccagcagatc ttccgtttcc ggcagttctt cggcgagggt aaaaaaatcc tcagcagaac
    15781 cgagatcttc catcagctgg cgggcctcgg aagagtcccg ctgccagacc gcattcagcc
    15841 gctgttcaaa ttcggcttcg tcgatttgcc gcagcgtaaa gggcgcgttc agcccccgct
    15901 gcagctcctg caaaacagag agcgacaacg gatgaacgtg gaggatctcc agcgacgctt
    15961 cgcaccatgc caccaggcta aaccgacggc tgaaactgta gggcagacgc acggtattag
    16021 cggtggtttc ctgtgctaca ggcaccatta acgcgttctc ccggcattga ggaatgcgcg
    16081 aacttccggc ggtaaggcct ggttttgcgc tggcagtacc ggctgcgcgg tgtgcggcat
    16141 caggcttaag ccttgatcat cgcgatagat ctgctcggcg cgcatgtagt tatatttgcg
    16201 ctgcgacacg ccgtctgccg ccataccgtc acgcagaatg gtcgggcgga taaacaccat
    16261 caggttacgt ttttcttttt tatccgccgt cgatttaaac aggttaccaa tcaacgggat
    16321 atcgcccagc agcggcactt tcgccacgct ttctcccgcc tggtcgtcca tcagaccgcc
    16381 aagcacgatc agttcaccat cgttagccag cacggtggtc ttcagtttgc gctcaccaaa
    16441 caccacgtcg aggctggtct gtccttccac cttcgacact tcctgctcaa tcaccatctg
    16501 taccgcgttt ccttcgttaa tctgcggcgt gactttcagc atgatgccga cttttttcct
    16561 ctctaccgta ttgaaaggat tgctgttatt ggagccaacg gtagatccag ttaataccgg
    16621 aacgtcctgg cccaccatga agaaggcttc ctggttgtcc agcgtggtga tgctcggcgt
    16681 ggagagcacg ttcgagctgg agtcgttttt gaccgcctgt accagtgcca tccagtcgcc
    16741 tttcaccacg ccaaccgccg taccgctaaa gccagaaagc agctgggcga gcgtggagag
    16801 atcgccgttg gtatccgggt taatggtggt ggcaccgttt tcactgatca ccgtggagcc
    16861 tttctgcggt ttcgcctgag aaatggctgc ccccagcgtg ccgataggga tctgcgtacc
    16921 gttagcaaac tgcattaatc cggcatcttt cgacgcccac tgcacgccga aattgatatt
    16981 gctgccttca gcaacttcca cgatcaacgc ctcgacgtgc acctgagcac ggcgaatatc
    17041 cagttgttca atcacgcttt gcagcgactg catgatgtcc tgcggcgcag taacaatcag
    17101 ggcattactg tgtttgctgg cggcgatgga gacaacctca cgcccgctac cgacggtgcc
    17161 ttccgcctct tctttagccg ccgtgagcgt gccgctgacc tgcttcagca catcgaccag
    17221 atcttccgct ttgctgtatt tgagatagaa aacctggctg ttgccgctgc gctccatttc
    17281 tgagtccagc cgacggatca ggcggcgcat tttgtcccgc gtggccgggt caccactgac
    17341 aatcacactg ttggtgcgtt cgtcggcgac aatttgagat ttcagcgtcg caggttggtt
    17401 ctcgccgctg tttttcgtca ggctttccag cacgcgggcg atttccgaag ccgaggcgtt
    17461 atccagcggg atcacctctt cagtgcgatt acccgcgtga tccacacgct ggatcacttc
    17521 cgtcagccgc tccacgacgg aggcgcgccc ggtgagcata atcacgttgg agggatcgta
    17581 attaacaacg ttgcctgagc ctgcgctgtc aatcatctgg cgcagaatcg gtgccagctc
    17641 gcgtaccgac acattgcgta ccggcacgac tttggtaacc atttcatcgc ccgcgtagtt
    17701 gtcgctgcct tcaccgacca gcggcagcgg ctcgactttc gcggcgcttg atttcaccac
    17761 cttcagcacg tcgttttcca tcggtacgac cgcatacccc tgtgcttcca gcaggttaag
    17821 gaataactgg taatactggc gttcattgag cggagtcata gtgcgaatac tcactttccc
    17881 ctgaacgccc ggccccataa tgatggtttt attaaggtta gcgccgacgg tttcgatgaa
    17941 cgatttcagg tcagtatctt taaaattagc ggtgaaagtg gcttcttccg cccagaccgg
    18001 tgaactacac aatgccgctg ccagcatcag cggcagtaaa cgcttttttg ttttgaggcc
    18061 agttgtcttc ttacgccaga tagacaacgt catatcacgc caaaacacga tgaatgattc
    18121 tcctggatta aatgcggtta gcgcagtgcg atggaaatgt cgtagcgcgc acccttgcgt
    18181 aaaaccgtaa gttgaatgga atccattgaa ggtaactgcc gcatcagagc aatcattgct
    18241 cgtggatcag tgaaatcctg ctgatttagt gcaatggcga tatcgccttc cctgaaaccg
    18301 ctggcatcga acagagaacg atctgccccc ggcttcactg cataaccgac aattccctcc
    18361 ttacgcacag gcgtaagctg gatatagtta aaaattttct gcggatcttt cgccagtgcc
    18421 tgacgcacgg cagccgggat ctcaactggc gcactggcag caggctcagc aacagcttgc
    18481 tttgcttcgt cgctgacggc ttttttgctg gtgaccgcca caggggggcg ttcctcctct
    18541 gccagactca gacgttccat tttcccctga tagcgcagca tcacatggtc gcggttgatt
    18601 tcctcaatca ctgcgttgtg agagccaagc cgttcaccct gcaaatagac ctgctgtttg
    18661 ccgccttctt caatcaccgc gccgggtctg gcaccaaagg cgatcccacg cagcaccaca
    18721 ttaagacgcg tttctgccac aggcgcagat tcaggttgtt ttaccggtgc ggcgacgggt
    18781 tgatatttgc caaaccagtt ttgttggctg actaattgca cgtcattttt atcgaacggt
    18841 tttgcatccg cacggagcgg tttattcgcc gatgaggaaa ccaccgtata ttccgcagaa
    18901 aaggagaaat agcgccagag tgaatacgcc atttttgcag aaataataag cagcatgagc
    18961 cagaacatcc cgcgcaccat ttttcgcaga tgctctttgt ctgtattaag agattgtctc
    19021 tggctaagaa tgtgacgaat tttagtcagc cattgaatga gataaattcg tgcgtcacga
    19081 aaaacaaccc gcgccaattt atatctaccc gacgttatgc tttgactatt ccacaggtgg
    19141 tacgatccag tttccgctga tactggttac ctgtacgtgt atcattaatc gttatgctgt
    19201 aattaattcc ctcggtgatc attaatttca ccgttgggtc agcgcacatt tgtcgttgat
    19261 agctggttaa aaaggcgtca ggtgtctgcg tggtctgagt acccgattcg ctgataatgg
    19321 tcatttttac cgtcgtgcca ctactttgcg ccagcactaa ggtatatccc gcagatttaa
    19381 tcggcaggtt ttggctgata ttttgcgcct gttttttcgc cagtaaactg gcgttttcat
    19441 tatggctggc gcagccactt aataatcctg taacgctcaa caatagcgat attaataccc
    19501 tccctggcat ttgttttatc gacatggtaa ataatctcta aggttattaa taagagttaa
    19561 aatgtcactt tgataatgac gttgttatca ttaaaacaat gcctttagat aaagtgttgc
    19621 tataccgccc agacttaaac acggtccaaa aggcagtgtg gttgatcctc tttttgtaat
    19681 aacggcatat atcaggccgc agcatgaggc gattaaagca acgttgggca gtgataacgc
    19741 ccccacccag ccacctaaag cggcgaagag taatacatcg cccatgccta atgcttcttt
    19801 acgcagaact attccggcta tccagcgcag ggagtaaaaa gtgataaatc ccaccagtac
    19861 gccggtgact gcgtcttgta gcgtgagcgg actctgctgc gcccatgccg caattagtcc
    19921 cgtccacaat acgccctgag taaaaacatc gggcagccat tggttatcga ggtcaatgac
    19981 gctcgcggca atcagccagg cggataatat catcaccgcc agcgcccatc cactttccgg
    20041 ccacaccagg ctcgccagca aaaaagcgag tgccgtcaat aactccacca gcggataacg
    20101 cttgctgatt ttcgcctgac agtcgcggca gcgccctttg agcatcaacc aggagagcag
    20161 cggaatattg tcacgaacgc ggatagtctg ctggcaatgc ggacagtgcg agcgtggcag
    20221 cgccaggctt atttttgact gcgtactcgg catttcaccg tgaaactccg ccatttgttg
    20281 gcgcagcatg atggggtaac gccaaatcac cacattcaaa aaactgccga taatcaatcc
    20341 tccgacggta gccaggacgg gcatcgccgc ggggtattgc tgaaaaacat caaaaaacat
    20401 ggttaaaggt tatttgttgt aacttgccgg atgcgggcct gcaggtgtat gccatacggc
    20461 tttcctccag gcccgatgcg ccttatttca tgccggatgc ggcgcgagcg ccttatccgg
    20521 tatacaggct tactcagctg acatcttatg ctcggtaacc tgattaatgg tttccggccc
    20581 ttgctgcggt ttcggcagat taagcgccgc cagcgtctcg taagccgact ggctcacacc
    20641 gccctcgaag ttcatctcgc tcgctcccgg caactggtaa gcattcgcgc ccggattcca
    20701 tttcttaaag aactccgaaa gatccgtctg ggcgacccag gaggcacaca gcatcagcgt
    20761 gtccgctgcg ttaccgttgg attcagcaca gtagttctta ctgccgaact tgttattgcc
    20821 aacctcatcg ccgcgcgctt tacggtgcat caactggaac aggttccagc ctttcatccc
    20881 ttcacgctcg ctgtaaaact taggcaggtc accttctggg taccactgtt tgatatcaaa
    20941 gtttttctct gcccactctt tcagctgtgc gtacatcagc agacggtccc ccgcaccgcc
    21001 gcgcgcccag gcctgaccgt tgctctcgtc cagatattcc ggcgcgacgg taatgtcgtc
    21061 agcgacacgg ttcatcttgc cgagatagcg atcctgcatg tacagcgcca gcacgttgtt
    21121 cgccacttca gttgcacccg gtacgttcag cggtgtttct gcagcgttat gaccggcttc
    21181 gtgccaaatc agccagtcgt tcagcggcgt tgtcggcagc gtggtgctgt tcgtcgagaa
    21241 gctgctgttc attaccggat aacccgagtg cgcatcaccg atggagatct gcacatcgtt
    21301 ggcgaaacgg tgtttatgcc ccgtcaatgc ttcataggta aacatccggt ggttaccgct
    21361 cgtttcattg cgaccgtaga agtcattcat cgagctggca aaggtatcca gatctttagc
    21421 gaattctgct acaccaccag tgaaattgct ggcctcaagg ttcttcttcg gcgtggtata
    21481 gacgaacgac gcagactcca gctcgcccag cggcgccggt gagtccagat cgtttttcca
    21541 ttcgccgtct ttatagaacg gcgcttttac tacaccggta aaggtgaagt tagcagaaac
    21601 atcatcctta ctgtcgccct tgatataaat cagaccacca taaggcacct tgaaggtcac
    21661 ttcaccgtta gcctccagag tatacgtttt agtcactctt ggcggacggt tcagcgcaac
    21721 ttcatgcttc tcacgtccag tcaggtcgtc agccagcgcc acggtaacag tcactgggac
    21781 tgacgccgaa gacttaatgg tgacgtcctg ctgggccggt gcccacaggc cggttgactg
    21841 catgttaccc gcaaaccatt tggtcggatt cgagtacagg ctgatgtttt ccgtaacgct
    21901 ctcacccttt gccgatacgg atcctgggta cttctccaca tcaaccttaa tgttcagatc
    21961 ccaccaggaa cggcccagca tcagacgcgt cagcggtttt tccatatagt tgagcggata
    22021 gcttgggttc atcatgcccg ctttgctgct accgtcaccg tagatcatgt tgttatcgac
    22081 cagcgatttt ttcagatctg cggagcactt ggtgccgcct gcataggcat cattggcgta
    22141 gcagttcagg aactcggtga acgttttaaa gcccagctca tcttccttgc cctcttcgta
    22201 acgatatttc gtatcgttcc acagccagac cgacatgttc tggtacagac gttccagatc
    22261 aacactgttc agacgctcac ctttactgcc tttggtacgg aaataaagct catgctgata
    22321 caggcgctga atgttggtgc ctaaatccgc cgcctgcacc atcgctttcg cggtgtcggc
    22381 gtcaagattc agttgcgtat agcgcggaac atacatgcca cctgttaccg gaacatccgt
    22441 gcctgggcgg cgctccaaac agttaatctc gtaatggtaa gtcgagtcct tacactcctg
    22501 taacccagga aacttctcaa agatttttgc ctttgccgct tccagagatt cttctgttgt
    22561 gtattccgct tcatcaataa aggcataacg cgttacctgt ttgccctcaa cttcctcctg
    22621 ccagctcgca acttccagtt tcggcttgtc atcaggcttg ttgtcttgct ggtatttcca
    22681 ggtcacttcc cctgtatttg ggtcgatggt gtacggcggt tgcgcgccgt ctgcagcagg
    22741 ataacgttca taaacccaaa tgccagtcgc gcgacgctga cgaacgcgat ccggataccc
    22801 ttgcggatcg ttgttcacca ccgatttgtt cagagccatt gacagacccg cggcatccag
    22861 cagacgcaca aaactggacg cgctctcttc cttaagattg ctcatcacgt tttccatgat
    22921 cagcaccgag ccacctttgt tcagataagc gatcagatcg gtcacatcct gctgagtcag
    22981 cttcggtttg ctggtatctg cacgcagagg cacagcatag ggatcgccag accactgagt
    23041 cacatattca aagccgttga ggatcagcaa cggaatctct tccggattca gatcgccata
    23101 gctggtcaac tgtttaaccg tgataccagt gaaatcctca tggaaagcaa atggtgcact
    23161 atttcccaat acctggcccg cttttttgaa ataaacgttc tccaggttgg tgccgacagt
    23221 catgatgctc ttggtatttg gctgccagat gtcatttgac aagtagcgca ggacgttctg
    23281 cataaagtgc ttcatgtcat cagaatcacc gctgagcgta cactcacctt tactattaac
    23341 accaccgccc caactgtaac cgttcgggca acgcaggatg ctgttgtagt gcgggttacc
    23401 gataaccatc agtttgcctt caccgacttg ccccagcgaa ataaacggca ggttgaaagt
    23461 cgcagtatcg cgcgtaacgt tctctggctg cacaatggaa ggcgcttccg taatgtacgc
    23521 cagctcattt ttatcccagg cgcgtttttc gccaaacgcc agccagtagt ttttatcatt
    23581 acgcgccatc agaatcggga atgccgagtt ggagatattt accaccgcct gaccgcgcgc
    23641 gttaccggtg ctgccataga agttggtaga gtcatggaag acgtggaact tgctgacaga
    23701 ctgatagttc gtatccacgc cccacagctt gttaataacg ccctgaatct ggccgtcatt
    23761 aacattgcgc gttgtcagcg agaaccagcg agcctcgtta caaccgtcgg ttttcgcaca
    23821 aatcgcggta tcgatctctt tggcctgacc cgtcttaaac tgctcgataa attcgttagg
    23881 cagcacaacg ttttgatcgc cttcatccag cgtcgcaccg ttggataacg aaagattgat
    23941 tatctcgttg atcacgttgg gatattcggc aaagaccttg cgtacatagt ccggaacaac
    24001 acgagtatta ttttgaccag tcgtcgaata acgatgaatg agctgatcga tatttgcccc
    24061 gcgaacttca tcacccaatt cagtcagcgc aatggtcgac ttattgccac gtactgagcc
    24121 cagttcaaag gtatcgatac caaaggagat gtttcgcccc agctaaagga gaatttaccg
    24181 ttttcgtcag ttacgccacg gcctgaattg gtgtagtagt caacgccagc aacaccgttc
    24241 cccaggctat ccaccagttg gccttcggaa agaatgattt cagtgggttg atactgataa
    24301 aactgttccg cgttagccga cacgaaggac gcgttcagat ccggttttgt tccctctgtc
    24361 gtgactggca ctaccgttga ggtatgggtg gacggcgctt tatcagtcgc agcattgttt
    24421 tccacctctt cattgaccag cttgctgaaa ttgtctgttg ccagatcaat ttgcttatac
    24481 agtttttcaa atcgcgcgcg atcaaccact gacgagaaag taagacaaag ctgttctgca
    24541 tctgcggggc aactgtcgct ggacgtcacc agagagatgg cgttggtttt cttattttca
    24601 gaattcgcca gctcctgcgc gtcctccagg ctaaacgaca ctttgtcaac cgcacgcagg
    24661 ctacgcgcag cttctgactg ggtgttgaat gttgcaatgg tcgtactgcc caccacacaa
    24721 ctcacggtat tgcctggcgt aaaggtaaag ccatcgctgg attcaccatt acaggtagca
    24781 ccagttaccc gctggcttcc gcccagggtc agataacccg ttttcgtagg aacaggttct
    24841 ggctccggtt ctggctctgg atcaggtgtc ggttccggcg taggttctgg gtccggcgtt
    24901 ggctcaggcg tcggctccgg gtttggtgtt ggatcaggtt tcacttccgg caaagaccct
    24961 gttccagaat ctacaggcgg cgtatcggag gaagatccgg aaccaccgcc atcacaaccg
    25021 gctaacaggg ttgcgctcaa aatagccgct
//
LOCUS       DAZKJR010000071        24876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKJR010000071 DAZKJR010000000
VERSION     DAZKJR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.071"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..184)
                     /gene="yohP"
                     /locus_tag="ACHOWS_002892"
     CDS             complement(101..184)
                     /gene="yohP"
                     /locus_tag="ACHOWS_002892"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFQ0048438.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            408..1844
                     /gene="mdtQ"
                     /locus_tag="ACHOWS_002893"
     CDS             408..1844
                     /gene="mdtQ"
                     /locus_tag="ACHOWS_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFQ0048439.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQHTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPVMQVAEQRIQLAEAQAKAIATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVDAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1897..2658
                     /gene="yohF"
                     /locus_tag="ACHOWS_002894"
     CDS             1897..2658
                     /gene="yohF"
                     /locus_tag="ACHOWS_002894"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0048440.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGARAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVRPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2777..3355)
                     /gene="yohD"
                     /locus_tag="ACHOWS_002895"
     CDS             complement(2777..3355)
                     /gene="yohD"
                     /locus_tag="ACHOWS_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFQ0048441.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILAVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3525..4112
                     /gene="yohC"
                     /locus_tag="ACHOWS_002896"
     CDS             3525..4112
                     /gene="yohC"
                     /locus_tag="ACHOWS_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFQ0048442.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4286..5218
                     /gene="pbpG"
                     /locus_tag="ACHOWS_002897"
     CDS             4286..5218
                     /gene="pbpG"
                     /locus_tag="ACHOWS_002897"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFQ0048443.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5257..6972)
                     /gene="dld"
                     /locus_tag="ACHOWS_002898"
     CDS             complement(5257..6972)
                     /gene="dld"
                     /locus_tag="ACHOWS_002898"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFQ0048444.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEYLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGVGKTSKRKN
                     WQEVE"
     gene            7168..9465
                     /gene="bglX"
                     /locus_tag="ACHOWS_002899"
     CDS             7168..9465
                     /gene="bglX"
                     /locus_tag="ACHOWS_002899"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFQ0048445.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATAKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9614..10531
                     /gene="osmF"
                     /locus_tag="ACHOWS_002900"
     CDS             9614..10531
                     /gene="osmF"
                     /locus_tag="ACHOWS_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFQ0048446.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10538..11695
                     /gene="yehY"
                     /locus_tag="ACHOWS_002901"
     CDS             10538..11695
                     /gene="yehY"
                     /locus_tag="ACHOWS_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFQ0048447.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACVIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHLLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGISGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11688..12614
                     /gene="yehX"
                     /locus_tag="ACHOWS_002902"
     CDS             11688..12614
                     /gene="yehX"
                     /locus_tag="ACHOWS_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFQ0048448.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLVEA"
     gene            12619..13350
                     /gene="yehW"
                     /locus_tag="ACHOWS_002903"
     CDS             12619..13350
                     /gene="yehW"
                     /locus_tag="ACHOWS_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFQ0048449.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKSMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13331..13438)
                     /gene="yohO"
                     /locus_tag="ACHOWS_002904"
     CDS             complement(13331..13438)
                     /gene="yohO"
                     /locus_tag="ACHOWS_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFQ0048450.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13498..14229)
                     /gene="mlrA"
                     /locus_tag="ACHOWS_002905"
     CDS             complement(13498..14229)
                     /gene="mlrA"
                     /locus_tag="ACHOWS_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFQ0048451.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14451..16136
                     /gene="btsS"
                     /locus_tag="ACHOWS_002906"
     CDS             14451..16136
                     /gene="btsS"
                     /locus_tag="ACHOWS_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFQ0048452.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            16133..16852
                     /gene="btsR"
                     /locus_tag="ACHOWS_002907"
                     /gene_synonym="yehT"
     CDS             16133..16852
                     /gene="btsR"
                     /locus_tag="ACHOWS_002907"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFQ0048453.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16899..17369
                     /gene="yehS"
                     /locus_tag="ACHOWS_002908"
     CDS             16899..17369
                     /gene="yehS"
                     /locus_tag="ACHOWS_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFQ0048454.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17411..17872)
                     /gene="yehR"
                     /locus_tag="ACHOWS_002909"
     CDS             complement(17411..17872)
                     /gene="yehR"
                     /locus_tag="ACHOWS_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFQ0048455.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(17997..20000)
                     /gene="yehQ"
                     /locus_tag="ACHOWS_002910"
     CDS             complement(17997..20000)
                     /gene="yehQ"
                     /locus_tag="ACHOWS_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0048456.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGKCLCQLAALTSRLNSAQEIARRDSVGEVPPMPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEHSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(19997..21133)
                     /gene="yehP"
                     /locus_tag="ACHOWS_002911"
     CDS             complement(19997..21133)
                     /gene="yehP"
                     /locus_tag="ACHOWS_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFQ0048457.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGDSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(21126..23405)
                     /gene="yehM"
                     /locus_tag="ACHOWS_002912"
     CDS             complement(21126..23405)
                     /gene="yehM"
                     /locus_tag="ACHOWS_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFQ0048458.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPYAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREDFMARWIGWAMQQNNGDVLVVCGGWHASALAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISFPAE
                     LTLNRFTPDGLAQSQVLHRLVILEIPGVVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFNQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLVQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(23416..24504)
                     /gene="yehL"
                     /locus_tag="ACHOWS_002913"
     CDS             complement(23416..24504)
                     /gene="yehL"
                     /locus_tag="ACHOWS_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFQ0048459.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5789 a         6351 c         6787 g         5949 t
ORIGIN      
        1 gcgccagtga ttatatgaag caatgcacat gacaggagcg caacaggatg agagactgaa
       61 atattctcgt tattttcgga tctgatgaca ttaattaatt ttaaaatatc atcttaaata
      121 cgccagtcac caccagtagc ccaatcagga aaataatcaa tacagcccag agtataattt
      181 tcattggcgt tcctttattt gtgagtgctg aataagtata ggaaaaaacg aagccctttg
      241 ccgctatcca taaacgatta agataaatcg gaagatatca ttcacttggt gtatattttt
      301 ctggatatgt cgttttattg ccactgatgt attgccacgc gaaactaaaa aatatatcgc
      361 aacacacggt aatctccgcg caccttttct tacctgatta ttcccccatg aatcgtgatt
      421 ccttttatcc agccatcgcc tgttttccgc tgttactgat gctggccggg tgtgcgccta
      481 tgcatgaaac ccgccaggcg ttaagccagc acacgcccgc tgtgcaagtt gacaccgcat
      541 tacccacggc gctgaaaaat ggttggccag acagccaatg gtggctggag tatcacgata
      601 atcaactcac ctcattaatt aataacgcac tacagaacgc accggttatg caggtggctg
      661 aacagcgcat tcagctggct gaagcccagg cgaaggcaat tgctactcag gatggtccgc
      721 agctcgactt ttctgcggat atggagcggc aaaaaatgtc ggcagaaggc ttaatggggc
      781 cgtttgctct gaacgatccg gccgcaggta cgaccggtcc gtggtacacc aacggtactt
      841 ttggcttaac ggcgggctgg catctcgata tctggggaaa gaatcgggcg gaggtaactg
      901 ctcgcctggg tacggttaaa gcacgagcgg cggaacgcga gcaaacccgc caattgctgg
      961 ctggcagcgt agcccgcctg tactgggagt ggcaaaccca ggcggcgtta aacacggtct
     1021 tgcagcaaat cgaaaaagag cagaacacca ttatcgccac cgatcgccag ctatatcaga
     1081 acgggattac ttcttcagtt gaaggtgtgg aaaccgatat taatgccagc aaaacccggc
     1141 agcagctcaa cgatgtcgcg gggaaaatga aaattattga ggcacggtta agtgcactta
     1201 caaatcacca gacaaaggca ttgaagctta atccggtcgc gttgccgaaa gtggcaagcc
     1261 agcttcctga tgaactgggg tactccttac tggcccggcg ggcagatttg caggcggcgc
     1321 actggtacgt tgagtcatcg ttaagcacta ttgatgcggc aaaagcggca ttttatcctg
     1381 acatcaacct gatggccttc ctgcaacagg atgcgttgca cttaagcgat ctgttccgtc
     1441 attccgcgca gcaaatgggc gttacggcag gcctgacgct acccattttc gatagtggtc
     1501 gtcttaacgc caacctcgat atcgcaaaag ccgaaagcaa cttgtctatc gccagctaca
     1561 acaaagcggt ggttgacgcg gtgaatgacg tggcgcgggc agccagtcag gttcagacac
     1621 tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga tgccttgcgt gtagtaggtc
     1681 ttgcacaggc gcgctttaac gcgggcatca ttgccggttc ccgcgtcagc gaagccagaa
     1741 tcccggcgct tcgcgagcgg gccaatggcc tgttattgca agggcagtgg ctggatgcct
     1801 ccattcaact cactggtgcg ttgggcgggg gttacaaacg ctgatgatat gcgccttcta
     1861 tacttaacgt ttattcagcg ttaagtggag aactcgatgg cacaggttgc gattattacc
     1921 gcctccgatt cggggatcgg caaagagtgc gcgttattac tggcgcagca ggggtttgat
     1981 attggcatta cctggcactc tgatgaagaa ggggcaaaag atactgcgcg tgaggtggtt
     2041 agccacggcg cacgtgcgga gatcgtgcag ctggatctcg gtaaactgcc agaaggtgcg
     2101 caggcgctgg agaaactcat tcaacgactg gggcgcatcg atgtgctggt gaataatgcg
     2161 ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg atgagtggcg caagattttt
     2221 accgttgatg tcgacggtgc attcttatgc tcgcaaattg cggctcgtca gatggtgaaa
     2281 caagggcagg gcggtcgcat catcaacatt acgtcggtgc atgaacatac gccgctgccg
     2341 gatgccagcg cttacacagc agctaaacat gcgctcggcg ggttaaccaa agcaatggcg
     2401 ctggagttgg tcaggcataa aattttggtg aacgcagtcg cacctggggc gatcgccacg
     2461 ccaatgaacg gcatggatga tagcgatgtg aggcccgacg cggagccttc gattcccttg
     2521 cgacgttttg gcacaacgca tgagattgcc agcctggtgg tatggctttg ttcggagggc
     2581 gcaaattaca ccaccgggca gtcgttgata gtggatggcg gctttatgtt ggcgaatccg
     2641 cagttcaacc cagaatagcg cagtgaggtg cgtggttatt tcaatgggtt aggccagata
     2701 agacacgcca gcgtcgcatc aggcatggaa catccggtgc cggatgtggc gtgaatgctt
     2761 atctgtggca actgctttac gcctgattat ccggcttttt cttcccgcgt cgtttcagcc
     2821 accagcgcac gcctaccacc agaaccacgg ccagaatcaa ccagacccag tgcttcaaat
     2881 gctggtcgag attgtgcaac cacggcgcaa tcacctgacc accagcataa ccgatagtgg
     2941 taaaaatcaa cgcccaggca aatgcgccga gaatattcag cggcagaaag attttcggcg
     3001 gcagctggct ggcaccaatc agcgtcgggc caatcaccct aaagccatac ataaaacgcg
     3061 taccaatgac aaacagatac ggatggcgtt ggataagttt ctgcgcccgc tcaattttat
     3121 cctgatgttt cgagaaacgg cgtaatagct tgccgccaaa gcgccgcccg cacagataaa
     3181 gcacctggtc gccaatcatg ccgccaagcg ccacagaaag taccaccagc gggaacttta
     3241 atagcccctg atgtgccgca acgcctccca gcaaagtcac ggtttcacct tccgccaggc
     3301 taccgatcac cagcgcggca taaccatatt gtgagatgag tgtattgaga tccataggcc
     3361 agtctgtctc cctttgcttg cctgtaagca tacaccctgt gaatagttca cgctgaaaaa
     3421 ggacgtacat cagacggtgc aaattacatg agcgaacaaa aaataatcac aagctgactt
     3481 atacttgaac tgtcaggttc atgaactcac aacaaggggg cggtatgagc catgtctggg
     3541 gactgttttc ccatcccgat cgtgaaatgc aggtgattaa tcgcgaaaac gaaacgattt
     3601 ctcaccatta cacccaccac gttttgctga tggcggcgat tccggtgatt tgcgccttca
     3661 ttggcactac acagattggc tggaattttg gcgatggcac tatcctgaag ttatcctggt
     3721 ttaccggact ggcgctggct gtcttgtttt acggcgttat gctggctggg gttgcggtga
     3781 tggggcgggt catctggtgg atggcgcgta attatccgca gcgtccgtca cttgcgcact
     3841 gcatggtctt cgcgggctac gttgcaactc cgctgttttt aagcggtctg gtggcgcttt
     3901 acccgctggt ctggttgtgt gcgctggtcg gcacggtggc gctgttttac accggctacc
     3961 tgctgtatct cggtattccg agcttcctga atatcaacaa agaggaagga cttagcttct
     4021 ccagttcgac actggcgata ggcgtgctgg tgctggaagt gctgctcgcc cttaccgtta
     4081 tactatgggg ctacggttat cgtttgttct agaaagttct tttgcgctgc attgctggcg
     4141 taaatgtcag caatgcagca tttcctcacg attcttcttt gacgatcgca ctttttgctc
     4201 gttatgatgg cgtcgctagc ctcagtaaat ccttactccg gcggtgcgca acccgtgcgc
     4261 gtgaaccact atctgaatgc tcatcatgcc gaaatttcga gtttctttat ttagcctggc
     4321 cctgatgctg gctgtgcctt tggcaccgca ggccgttgcg aaaacggcag ccgctaccac
     4381 cgcttcacaa ccggaaattg cctccggtag cgcgatgatt gttgatctga ataccaacaa
     4441 agtgatctat tcgaaccacc cggatctggt gcgtccgatt gcgtctatca gcaaattaat
     4501 gaccgcgatg gttgtgctgg atgcacgact gccgctggat gaaaaactaa aagtggatat
     4561 cagccagacg ccggagatga aaggggtcta ttcgcgcgta cgactgaaca gcgaaatcag
     4621 ccgtaaagat atgctgttgc tggcgctgat gtcttcagaa aaccgcgcgg cggcaagcct
     4681 tgcgcaccat tatcccggtg gttacaaagc ctttattaag gcgatgaatg cgaaagcgaa
     4741 atcgctcgga atgaacaaca cgcgctttgt tgaaccgacc ggattgtcgg tgcataacgt
     4801 ttcaactgcc cgtgacttaa ccaaactgct cattgccagc aaacaatatc cgttgattgg
     4861 tcagttaagt actaccaggg aagatatggc aaccttctct aacccgacgt atacgctgcc
     4921 gttccgcaat actaaccatc tggtgtatcg cgataactgg aatattcagt taaccaaaac
     4981 gggctttacc aatgcggcgg gccattgtct ggtgatgcgt acggttatca ataataaacc
     5041 ggtggcgctg gtagtgatgg acgcgtttgg caaatatacc cattttgccg atgccagccg
     5101 cctgcgtacc tggattgaga ccggtaaagt gatgcctgtt ccggcagcag cgttgagcta
     5161 taaaaaacaa aaagccgccc aaatggcggc ggcggggcag acggcacaga acgattaaat
     5221 gaatgacgga tggcgatact ctgccatccg taatttttac tccacttcct gccagttttt
     5281 ccgcttactg gttttaccaa cccccggatt catactgttg gtcggatcgt tctcgcgata
     5341 aaacttctgc aacgtttccg gtgctttata caaatgaccg acgttatgct cggcagggta
     5401 ttgcgcgccg cgctgctgta gcagttccag catctgttct ttcaacgcat gcacatccac
     5461 gcctttttta acgatgtaat cctgatggaa gacatagcac ataaaatggc cgtaatagag
     5521 cttgtgcacc agctggctgt cgatctccgg cggtaaatac tcataccact cggtgtcgtt
     5581 acgccgcaga gcgatatcca gtgccagaat gtcttcgact tcatcggaat gcaccgcctg
     5641 ataacgaatt gctgcgcccg cagcggcgaa acggtgtaaa aacgctttgc tgccttcctc
     5701 cggcgtacag acaaagaaat cgccttcggc ctgtttgaaa taatccacca gccacgattt
     5761 ggcttcaccg acgccatcgc ccgccatttt taacagcaga tgatgttcgt atttatcgcg
     5821 ccagtttttc atgcgcggcg gtaaatggct ggggaacagg tgaccgaatt tttgcatcgc
     5881 acggtcggta aaatgcggac ggaagaattt cactttctcc agcatcgcat cggtgcgccc
     5941 cttgagatta aagaagaacg gcatcttgtc ggtgcctaac ttatcaatca tcaggaaggt
     6001 gtctttgccg tatttttccg ctatatcgta gatatcccgg tgcatatatt ccccggcaac
     6061 cggcagattt tcgaaattag ccagaatatg acggcggatt tcggtcagta cttccggctg
     6121 gttggtgccg atataaaaca cctgctgatt tttttccgct tcgaaggtat caagacgtac
     6181 cgcaaagacg gccagcttcc cggcgcaacc agaagattca aataaccgat ccggatcggc
     6241 gttataacgt gcgggcgtgt cggcttcaat atcacgaacg cggtggacat agtcataatc
     6301 gtgggcgtga cgaccatcgt ggcgcacatc gtcatctttg atgcgatcgt catccagctt
     6361 gctaaggatc tgttccggcg tttcgcccag atcaatcccc agatggttaa ccagcgtcag
     6421 cttgccatct tcatttatac gcgcgaataa cgacatttcg gtatacgccg ggccacgttg
     6481 caccagcgag ccgccggagt tgttacaaat accgccgatg accgatgcgc ctatacacga
     6541 tgatccaatc accgagtgcg gttcgcgtcc cagcggtttg agggcttttt ccagcgaata
     6601 aagcgtggtg cccggatagg ccagcacctg ttcgcccttg ccgagaacgt gcagcttgtc
     6661 gagacgcagg gtgctgatga tcacgatatc gcgatcatag tcgttaccgt ttggcgtcga
     6721 tccttcggtc aggcctgtat tggcagcctg catcagaata attttgtcgg cggtgacgca
     6781 ggctttcagc acccgccaca attccagtag tgagccaggg aaaacgacag ccagtgcgtc
     6841 gccctgacca gaacggaagc ccttgcgata gcgggccgtt tttgcgggat cggtgagcag
     6901 gtgtgaatga ccgaccagac gggcaagttc attcaaaaag gctttattat cagttgttgt
     6961 catggaagac attttccact ccttgtggtg gcgaaaaaat atcacaaact agaatagcgc
     7021 cttgtatgac aattcagcga gtgaatattt tgaaaattca gacaataaac tgagcattct
     7081 ttttctctct ccatcatgct tatggcaaac tacggttttt gcaccatttt cgctgccacg
     7141 tcgggcaaca aaggaagaaa aatccatatg aaatggctat gttcagtagg aatcgcggta
     7201 agtctggccc tgcagccagc actggcggat gatttattcg gtaaccatcc cttaacgcct
     7261 gaagcgcggg atgcgttcgt caccgaactg cttaagaaaa tgacggttga tgagaaaatt
     7321 ggtcagctgc gcttaatcag cgtcggcccg gataatccga aagaggcgat ccgcgagatg
     7381 atcaaagacg ggcaggttgg ggcgattttc aacaccgtaa cccgtcagga tatccgcgcc
     7441 atgcaggatc aggtgatgga attaagccgc ctgaaaattc ctcttttctt tgcttacgac
     7501 gtgctgcacg gtcagcgcac ggtgttcccg attagcctcg gtctggcctc gtcttttaac
     7561 ctcgatgcgg tgaaaacggt cggacgtgtc tctgcttatg aagcggcaga tgatggcctg
     7621 aatatgacct gggcaccgat ggtcgatgtc tcgcgcgatc cgcgctgggg acgtgcttcc
     7681 gaaggttttg gcgaagatac gtatctcact tcaacaatgg gtaaaaccat ggtggaagcg
     7741 atgcagggta aaagcccggc agatcgttac tcggtgatga ccagcgtcaa acactttgcc
     7801 gcatacggcg cggtagaagg cggtaaagag tacaacaccg tcgatatgag tccgcagcgc
     7861 ctgtttaatg attatatgcc gccgtacaaa gcggggctgg acgcaggcag cggcgcggtg
     7921 atggtggcgc tgaactcgct gaacggtacg ccagccacct ccgactcctg gctgctgaaa
     7981 gatgttctgc gcgaccagtg gggctttaaa ggcatcaccg tttccgatca cggcgcaatc
     8041 aaagagctga ttaaacatgg cacggcggca gacccggaag atgcggtgcg cgtggcgctg
     8101 aaatccggca tcaacatgag tatgagcgac gagtattact cgaagtatct gcctgggttg
     8161 atcaaatccg gcaaagtgac gatggaagag ctggatgacg ctgcccgtca tgtactgaac
     8221 gttaaatatg atatggggtt gtttaacgac ccatacagcc atttggggcc gaaagagtct
     8281 gacccggtgg ataccaatgc cgaaagccgc ctgcaccgca aagaagcgcg tgaagtggca
     8341 cgcgaaagcc tggtgttgct gaaaaaccgt ctcgaaacgt taccgctgaa aaaatcagcc
     8401 accattgcgg tggttggccc gctggcagac agcaagcgtg acgtgatggg aagctggtcc
     8461 gcagccggtg tcgccgatca atctgttact gtgctaacag ggattaaaaa cgccgtcggt
     8521 gaaaacggta aagtgctgta tgccaaaggg gcgaacgtta ccagtgacaa aggcattatc
     8581 gatttcctga atcagtatga agaagcggtc aaagtcgatc cgcgttcgcc gcaagagatg
     8641 attgatgaag cggtgcagac cgcgaaacaa tctgatgtgg tggtggctgt agtcggtgaa
     8701 gcacagggga tggcgcacga ggcttccagc cgtaccgata tcactattcc gcaaagccaa
     8761 cgtgacttga ttgcggcgct gaaagccacc gctaaaccgc tggtgctggt gctgatgaac
     8821 gggcgtccgc tggcgctggt gaaagaagat cagcaggcgg atgcgattct ggaaacctgg
     8881 tttgcgggga ctgaaggcgg taatgcaatt gccgatgtat tgtttggtga ttacaacccg
     8941 tccggcaagc tgccgatgtc cttcccgcgt tctgtcgggc agatcccggt gtactacagc
     9001 catctgaata ccggtcgccc gtataacgcc gataagccga acaaatacac gtcgcgttat
     9061 tttgatgaag ctaacggggc gctgtatccg ttcggctatg gtctgagcta caccactttc
     9121 accgtctctg atgtgaaact ttccgcgccg accatgaagc gtgacggcaa agtgaccgcc
     9181 agtgtgcagg tgacgaacac cggtaagcgc gaaggggcga cggtagtgca gatgtacttg
     9241 caggatgtga cggcttccat gagtcgtccg gtgaagcagc tgaaaggctt tgagaaaatc
     9301 accctgaaac caggcgaaac ccagaccgtc agcttcccga ttgatatcga ggcgctgaag
     9361 ttctggaatc aacagatgaa atatgacgcc gagcctggca agttcaatgt ctttatcggc
     9421 actgattccg cacgcgttaa gaaaggcgag tttgagttgc tgtaatgctt tagtaagggc
     9481 gcgacgtctg cgccctttaa gtatttatct gattctgcaa ctgaatccgc ccagacaggc
     9541 taaccctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
     9601 tgaggaaagc atcatgccac tctcaaagct ctgggcaggt tcattggttt tgttggcagc
     9661 cgtgagcctg ccgctacagg cggcttcccc cgttaaggtc ggttcaaaaa tcgataccga
     9721 aggcgcgctg ctcggcaata tcattttgca ggtgctggaa agtcacggtg ttcctacggt
     9781 aaataaagtg caactgggaa cgactcccgt ggtgcgcggg gcgattactt ccggtgaact
     9841 ggatatctat ccggaatata ccggcaatgg cgcgtttttc tttaaagatg aaaacgatgc
     9901 ggcatggaaa aatgccgggc aaggctacga gaaagtcaaa aaactcgatg cagagcaaaa
     9961 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    10021 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgaaaga
    10081 gggcggcacc ttcaaactgg cggcctcggc agaatttatt gaacgcgccg atgccttacc
    10141 cgcttttgaa aaagcctacg actttaaact cgatcaggat cagttgctgt cactggctgg
    10201 tggcgacacg gcggtaacga tcaaagccgc tgcccagcaa acttctggcg ttaatgccgc
    10261 aatggcttac ggcactgacg gtccggtcgc agcgctgggg ttgcaaacct taagcgatcc
    10321 gcaaggcgtg caacctatct acgcgcctgc accagtggtt cgtgaatcgg tgttgaagga
    10381 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    10441 gcagcaactg aatgccagca ttgcagttga aggactggat gccaaaaaag tggctgccga
    10501 ctacctgaaa caaaaagggt ggacgaagta atttcccgtg acttatctac gtattaatcc
    10561 tgttctggcg ctgctgctgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    10621 cgcgcctaat cgtttagttt cgggtgaggg gcgtcatctc tggcagctgt ggccgcaaac
    10681 gatctggatg ctggtgggcg ttggttgcgc ctggctgacc gcctgtgtta ttcccggtaa
    10741 aaaaggcagc atttgtgcac tcattctggc gcaattcgtc ttcgtattgc tggtgtgggg
    10801 agctggaaag gcggcgacac aactggcaca aaatggcagt gcgctggcgc gtaccagcct
    10861 cggcagtggt ttctggctgg ctgcggcgct ggcattgctg gcctgtagcg atgccatccg
    10921 ccgaatctcc acgcatctgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    10981 gctgtggttg ctgtactccg gcacgcttaa cgatctctcg ctaatgaaag aatacgccaa
    11041 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    11101 gctgcctgcg ttagtgattg gtgtgccgtt gggcgtctgg tgctactttt ccactgcgcg
    11161 gcagggagcg attttttcgc tactcaatgt cattcagacc gtgccttcgg tggcgctctt
    11221 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tgggtaagct
    11281 cggcatatca ggaaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    11341 gccgctggtg cgcggcgtgg tagtcggctt gaaccagatc ccgcgcgatg tgctggagag
    11401 cgccagagcg atgggcatga gcggggcgca gcgattcctg catgttcagt taccgctggc
    11461 gttaccggta tttctgcgta gcctgcgggt ggtgatggtg caaaccgtcg gcatggcggt
    11521 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    11581 cagtgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgctgat
    11641 cgatgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    11701 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    11761 aggaggggag tttttcggtg ctgattggta catcaggctc cggtaaatcc accaccctga
    11821 aaatgattaa ccgcctggtg gagcatgaca gcggcgtgat ccgctttgcc ggagaagaaa
    11881 ttcgctcgct gccagtgctg gagttgcgcc gccggatggg ctatgccatt caatctattg
    11941 gcctgtttcc ccactggagc gtggcgcaaa atattgccac cgtgccgcaa ttacaaaaat
    12001 ggtcacgggc gcggatcgac gatcgtatcg acgaattaat ggcgctactg gggctggagc
    12061 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    12121 tggcgcgcgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcgc
    12181 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcatacac cgtttgctgg
    12241 ggcgtaccat tgtgctggtc actcatgata ttgatgaggc gctacgactg gcagaacatc
    12301 tggtgttgat ggatcacggt gaagtggtgc agcagggcaa tccgctgacg atgctgactc
    12361 gtccggcgaa tgattttgtc cgccagtttt ttgggcatag tgaactgggt gtccgcctgc
    12421 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcgc
    12481 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgtgaag
    12541 tgctgccggt ggtgaacacg cagggcgagc cttgcggcac gctgcatttt caggatctgc
    12601 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctttg
    12661 tttgtggcac tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    12721 caactgccac gccccgttta tcagcaagaa agttttgctg ctctggcact ggctcatttc
    12781 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcactggtgc aggaattatt
    12841 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    12901 cagacttttc cgcccgtcgc ggtgctggcg attgccgttc cggtgattgg gtttggtctg
    12961 caacccgcga ttatcgcctt gatcctttat ggtgtgctgc ccgtcctgca ggcgacactt
    13021 gccgggctgg gagcgattga tgccagcgtg acagaagttg cgaaaagtat gggaatgagt
    13081 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggt
    13141 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    13201 agcacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    13261 cagggggcgt tactggtggc actggcggcg atcattgcag accgcctgtt tgaaaggctg
    13321 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    13381 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    13441 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    13501 aatgccgagt gggaaaatat catggccttg ttcctgccaa ctggtgagtt gctgctgttg
    13561 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga acagttcagg
    13621 gcgtagttga ttgagcgagt gggcgaggac atcaatgcgc catccttgtt gactggcaat
    13681 ccagccctcc agccacagac gggtggtatc gtgaatattc cagccaacca ccagcgcatc
    13741 tttaccctgt tttttacgtg ccgaagccag gcaaatggcg atgtagttga tcagtacgcc
    13801 gtcgaggatc gccagcagcg cctggagagt cggttgttgg cactgaagtc gtcggcgcag
    13861 aggaataaac agatgcgtgg tgagtgtctg ggcggggtaa tcctgaccgc gctctttgat
    13921 ccacgttcgc aggctatgca gattgccgct ttgcaggtaa gtcagtaatg tttcttgctg
    13981 atcgcgccag ccgttctgca catcaacatt ttcattactg agcagcattt taactttgct
    14041 gacctgcacg ccgttgtcga tccagcgttt gatctcgcgg atccggtcaa tatcggcatc
    14101 attgaacagc cgatgaccgc cgtctgtccg ttgcggtttc agcaatccgt aacgcctctg
    14161 ccacgcgcgt aacgtgacag ggttaatatc acaaagcaac gccacttcac caattgtgta
    14221 aagcgccatc gtctcaccct tgctcgcgag gtcccggttt aactttagac gcagttttgc
    14281 gaaccaggta gttttgcccg ttttttgtgc atctataggg tgattttatt tttgccaggc
    14341 gattttgagt gatcgtactc acgaattctc atttttctgc aagagttcaa agaaagttaa
    14401 acgcaggcaa tgtatgttac gcgttttaaa gggaagtgtg gtttgcgggt atgtacgatt
    14461 ttaatctggt gttgctgctg cttcagcaga tgtgcgtttt tttagtcatt gcgtggttaa
    14521 tgagtaaaac gccattattc ataccgttaa tgcaggtcac ggttcgtctg ccgcataaat
    14581 ttctctgcta catcgtcttt tccatcttct gcatcatggg cacctggttt gggctgcata
    14641 ttgacgattc tattgccaat acccgtgcga taggcgcggt aatgggcggc ttactcggcg
    14701 gtccggtcgt cggtgggctg gttggtctga ccggcgggtt acatcgatat tcgatggggg
    14761 gcatgaccgc gctaagttgc atgatctcga ccatcgttga agggttgctc ggcggcctgg
    14821 tacacagcat cctgatccgt cgcgggcgca ctgataaagt ctttaacccc attaccgccg
    14881 gtgccgtcac gttcgtcgct gaaatggtgc aaatgttgat catcctggcg atcgcccgac
    14941 cttatgaaga tgcggtgcgt ctggtgagta atattgctgc gccaatgatg gttaccaata
    15001 ccgtcggcgc ggcgctgttt atgcgtatat tgctcgataa acgcgcgatg tttgaaaaat
    15061 acacttcggc tttttctgcc actgcgctaa aagtggctgc ctcgacggaa ggcattttgc
    15121 gccaggggtt taacgaagtg aacagcatga aagtggcaca ggtgctgtat caggagctgg
    15181 atattggtgc agtcgcgatt accgatcgag agaaattgct ggcctttacc ggaattggtg
    15241 acgaccacca tttacccggc aaaccgattt cttcgactta caccttaaaa gcgattgaaa
    15301 ccggtgaagt ggtctacgct gatggcaacg aagtacctta tcgttgctct ttgcatccgc
    15361 aatgcaaact ggggtcgacg ctggtcattc cgttgcgtgg cgaaaatcag cgggtgatgg
    15421 gcaccatcaa attgtatgaa gccaaaaacc gtttattcag ttcaatcaac cgcacgttgg
    15481 gcgaggggat tgcgcaactg ctttcggcgc agatcctcgc cgggcaatat gagcggcaaa
    15541 aagcgatgct cacccagtca gagatcaaac tgcttcacgc ccaggtgaac ccccattttt
    15601 tgtttaatgc gcttaacacc attaaagcgg tgatccgccg cgacagcgaa caggccagcc
    15661 agctggtcca gtatctttcc acttttttcc gcaaaaactt aaagcggcct tcggagtttg
    15721 ttactctcgc agacgaaatt gaacatgtga acgcttatct gcaaattgaa aaggcgcgct
    15781 tccagtcgcg gttgcaggtc aacattgcta ttccgcaaga attatcccag cagcaattgc
    15841 ccgcgtttac cctgcaaccg atagtggaaa acgccattaa acatgggaca tcacaactgc
    15901 tggatacagg gcgagtggcg atcagcgccc gacgtgaggg gcaacatttg atgctggaga
    15961 tcgaagacaa tgccggtttg tatcaaccgg taaccaatgc cagtgggctg gggatgaatc
    16021 tggtggataa gcgtttacgt gaacggtttg gcgatgacta tgggataagc gtcgcctgtg
    16081 agcctgattg ttacacccga ataacgttac gactaccatg gagggacgag gcatgattaa
    16141 agtcttaatt gtcgatgatg aaccgcttgc acgggagaac ctgcgcgtat ttttgcagga
    16201 gcagagcgat attgaaatcg ttggagagtg ttcaaacgcc gtagaaggga tcggcgcggt
    16261 gcataaactg cgcccggatg tgctgtttct cgatatccag atgccgcgca tcagtggtct
    16321 ggaaatggtg gggatgctcg accccgaaca tcgaccgtat atcgtttttc tcaccgcgtt
    16381 tgacgaatac gctattaaag cctttgaaga acatgccttt gactatctgc tgaagccaat
    16441 tgatgaagcg cgactggaga aaacgctggc gcgtttgcgt caggaacgca gtaagcagga
    16501 tgtttcgctg ttaccggaaa atcaacaggc gctgaaattt atcccttgta cggggcatag
    16561 tcggatttat ttactgcaaa tgaaagatgt ggcatttgtc agcagtcgga tgagcggtgt
    16621 ctacgttacc agccacgaag ggaaagaggg ctttaccgaa ttgacattac gtaccctgga
    16681 aagtcgtaca ccactactgc gctgccatcg tcagtatctg gttaacctcg cgcatttaca
    16741 ggagattcgt ctggaagata acggccaggc cgagttgatt ttgcgtaatg gcttaaccgt
    16801 gccggtcagc cgccgttatc tgaaaagctt aaaagaggcg attggcctgt aaaagactgc
    16861 taaaatggct ttttgcctca tcaacacctg aaggcctcat gctaagtaac gatattctgc
    16921 gcagcgtgcg ctacattttg aaagccaata ataatgacct ggtgcgtatt ctggcgctgg
    16981 gtaatgtcga agccaccgcg gaacagatcg ccgtctggct acgtaaagaa gacgaagagg
    17041 gttttcagcg ttgtccggac attgttttgt cgtcattcct caatggcctg atttatgaaa
    17101 aacgcggcaa ggatgagtct gctccggcac tggagccgga acgtcgcatt aataacaaca
    17161 tcgtgctgaa aaaattacgc atcgcgtttt cgctgaaaac cgatgacatt ctggcgatcc
    17221 tcaccgaaca gcagttccgc gtttcgatgc cggaaattac agcgatgatg cgcgcaccgg
    17281 atcataaaaa cttccgcgaa tgcggcgatc aatttttacg ttattttctg cgtggactgg
    17341 cagcgcgcca gcatgtgaag aaaagctaag acgggtatgg cggccatgca aaaacatggc
    17401 cgccgacaga ttatttcact tctttaaaac cagcggcttt catcaccagt tccatttgcg
    17461 ccatagtgat accttttttg gcatcttcag cagaaacgtt gattcctgaa ataccctgca
    17521 gggctttgaa atccactttt tccatatcga tagtcacgtt ttcctgcgcg taagtatcgg
    17581 tataggttaa tttttcttca acacccgcga tgtttttgta tttggcgctt aacggctcaa
    17641 gtgtcctggc agcgtcttct ttggtggttg caccaatgga ggcaaattga attttggttt
    17701 cagaagattg cttaagcacc ttgtcacctt tgtagacata ggtaatggca atttcagtac
    17761 cgttcagatt ggcgctgaat tttttcgatt cttctttgtc accgcagcca gcaagagaga
    17821 aaaccagaac agatgcaaca acgagggaaa acagcttatt gaaagccttc atgtaaaact
    17881 ccattttatt taatcaagga actggtgact ctcaccaggg gctatatagg atatgcctaa
    17941 taccgtggcg tgagcagtcc ggaactggag tagaactctt agtaaaaagc actatttcat
    18001 ccttgttgct gaagcatggg gaataattgt tcgcacagca aaacaccgtt attcattgct
    18061 tctacccgtg cctcgctttc tgtattacga aattgtccca acacatgtgc cagccgataa
    18121 aaacccaccg cggtgaggtc attcgccagc aactctgcct gaccaatagc actatgttcc
    18181 tgatagcgcc agccgttatg gagcagttga ataagtaacg cctggcagcg catcagcaac
    18241 tgatgagcag tagacggcac aggcagaacg ctggcagaag gtagtgatgc caccacaggc
    18301 gcagtttctg cgtccagcgc ccaggcacga gtctttgtca tcattacctg tggttccagt
    18361 gtcaattgcc cctctacaaa gctgacaaag ccagaaacca gacacacggg gtcgtctgtt
    18421 tgttgcaaaa gcgccgccat gcgttcaacg gcataaggcg cgctggctga ggctggcagt
    18481 gataacgtca gcagattatc ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg
    18541 cggctgctat cccaaccgag cgaaatacac tcagcgaccg gcagaataaa taagttatcg
    18601 acctgattaa gaggccgtat gcaggcgggg ggacgctggc gtaaatattc ccgcaaagcc
    18661 acaatgcccg gctggcgtaa cggcgcgctc aacatttgcc aggcatcagg cgacagcggc
    18721 acaacgctgc ttaagcggtt gcgggtagct aacagcagct cgccatcggc actgcgtttt
    18781 gctgcttgtg aaacaatttg cccgcccgcc agtgcgccag cctgaaaact aaacagccga
    18841 cgcgtcgctg ccggtgagtt ttcctgttcg cttctcggcc aactacgcga aagatgcaaa
    18901 atactgccgg tgtcgggatc ggtaaaccag atgcgtaaac cataatgctc aatatcctgc
    18961 cagcaacgca tacctaaaga caccagccgc agatgatcaa gctttgcttc tccggcaatg
    19021 ccagagccaa cgaccgtgcg ccacggcatg ggcggtactt caccgacact gtcacgtcgc
    19081 gctatctctt gtgcactgtt caagcgactg gttaacgccg caagctggca caagcatttt
    19141 ccggcatgat agtggctggc gcgggcgtgg aaggcatcaa cgctggcgcg cagttgccgt
    19201 agcgattcac tcacccatcg ccagttgcag cgttccgccg cctgctgtgc gcggctgaaa
    19261 gcggcctcgt aatggattgg cggctggctg atgccgccca gccataatgc ctgacttaat
    19321 tgctgaacat attgacgaca cgcgttgccc tcttcgctgg caaacggatc gtcagatgat
    19381 gtgacgtgtt cgctgcgcat ctgccagatt aaatgagtaa attccgcttg ctgagttttg
    19441 gcctcgacga aggcctgcac agccagtacg acatgttcgc aaagtgtgcc gtcaatacaa
    19501 tcacagcgag cgaaacgaat gctgctgcgg gaataaaaac gtacatcgct catcggtaag
    19561 cgggcagagg gaatttcgcc cggcgtacag aacaactcaa tggtgatgcc tttagcgacc
    19621 agcgcctgtg cgcgtttgcg ggtggcatcg ggcagggtag ccagttcttc cagccagatt
    19681 gccggatccc actcttcttc tttttccgta ggctgggtgg tggcgcaaag tcgctgataa
    19741 cttaaaacca gcatcacgcg atggcggcac atgccgctgg ccccgcagct gcactgagcc
    19801 tctttcagtg cctggccgtt cgccagctgg gtgcggacac cgtcactgaa ggtggcgatt
    19861 aaagcgccgt tctcatgact gatttccggg acgttgccat tttccagttc tttaaggctg
    19921 cgcttaacaa aaccggcatt gcttaacgcc gtcagtgcct gtggtgtcag ttctaataat
    19981 tccggacgta gtgaattcat gactgaagat tctccgcaag ccaggtcgcc agttcgcccg
    20041 gcgtcatggc ggctatttgt gcaccgacat taaccagtgc ctgggccata tcgcggtcat
    20101 agcaaggtgt tgcggtgcta tcgagcgccg ccagtcccag cactttgatg ccgctctgga
    20161 cacacttttt cacctgatgc gtcagcaatg atgatgaacc accttcatag aaatcgctca
    20221 cgaggataat gacacttttt gcaggttgtt caataagttg ccgaccatac tccacggcac
    20281 tggcgatatt ggtcccgccg cccagttgta ctttcattaa taactctacc ggatcggcaa
    20341 cgtctgccgt gagatcaacc acgctggtgt caaacgccac cagatgggta cgaatgccgg
    20401 gtaactgcca taaacaggcc gccatcaccg ctgagtggat caccgagtca accatcgatc
    20461 cgctttgatc aaccagtaag accagttgcc attgatcgct atggcgctta atgcggctgt
    20521 taaagcgggg ggattcgata tacaacttgc cgtgttgcgg atgccagtgt tgcaggttgg
    20581 cacgcagcgt acttttgaaa tcgaaattac gcgccagtgg aataaatgag cgacggcggc
    20641 gatcgcggac accagaaaaa gcctgacgaa cttcctttgc cagtcgcgcc ataatttctt
    20701 caacaacctg gcgcactatt cggcgggcgg cagccagcac ttcgggattc atcagatgtt
    20761 tggtgtgcaa aacggcgcgt agcaggcttt cagaaggctg catacgttcc agcacgtcga
    20821 gattcgtcac cacatcttca atgccgtagc gcagtacggc atcgctttcc agtcgctcga
    20881 tcacccgttg cggaaacagc gtgtgaatac tgttgatcca ctcaggggtg gtgagatttg
    20941 agtcacctaa tccaccggag cgttcaccac gctggagccg ttcaggatcg cgcccataca
    21001 gccactccag cgcgtggtct atctgccggg cgttgtcatc cagcccacaa agcgtcgttt
    21061 ctgccgcttc gccaagaatt aatcgccagc gttgtagctc acgggtggtc agaagatcgt
    21121 tcagttcaga catggaaaac tccccagtgt tgcagcgatg ccagtgcctg ctgttcgagt
    21181 tgttgatgat gtgcaatgtc ttgtggtgga caatgcaacg gcatttgcag tgccgaaacg
    21241 ggaagttgca ccagttgata atgctcaagc acctgatgcg ccagcgtccc gcgttctcgt
    21301 ggtggtagcc aggccatcgc cgcgcgtaaa tcgggcaggg cattgataaa atcggcatca
    21361 ctcagttgat ttaaatgact gctgaaaccg gcgataaata ccggctgaca ggccagttga
    21421 tgacgggcta atgccagcaa accgtgcagc gcctcgccgg attgtgtcgg ggataattgc
    21481 accagcattg tcagcgccgc ttcggcactg gcgttgggat gttccagacg catcagtgcg
    21541 ccaagtgccg cgccgcgatc cagcgccggg gcatgaatcg cctgactgcg tcgctccagt
    21601 aaggctaccg ccgcagaaag agaaacgccg ggtaaattaa cgccgctatg tagatcgcgc
    21661 aggatatggc aaagcgcttg ccagctatgc aggtgagcgt gaaactcctt ttcatccggt
    21721 ctgccgttgg attcacatag ccacagcgtg cgatcgatgg ccgcgcataa tgtcgtttgt
    21781 aatatctgcg cgccctgcat accgctaatt tcatccagcc gccataaggc atatagcact
    21841 tccagcgcct ggcccatttc ggcaaactga ttttcctggg cgattaataa tgtgagttgc
    21901 tccagtaatt gttgattgaa ggacgccaga cccgctaacg ccgcctggct aagacatgtg
    21961 gtgatactgc cgattccacc cgcgtccagc atatcggctt ctaatttatt gcgtgcggct
    22021 tcctggagtg tggcaccaaa gcaggcggcc tcaattaatg ccgcatgttg gctaagcggg
    22081 cgggttaatt tccagcgttc ttcaccgttg cctgcaagtg tcagtgtact tccctgctgg
    22141 cgcacaaccc ctgggatctc cagtattacc agccgatgta acacctgact ttgcgccagt
    22201 ccatcggggg taaagcgatt taaagtaagc tccgccggaa aagagattcc tgtacgttcc
    22261 agttcgcagg tgacatcttt tggcagaggc ggctgtggcg tagagggggc aagtttaccg
    22321 aatccgtcac ccgctaatgt gtctatcaac gttagcagaa tcggatcggt atcgggatga
    22381 ataacgccgc gatagctcca cggcaacggc gcattcaggg cttctttaat cagcgagcct
    22441 gctatggcat ccagccagtc agtgcgtaac ggtagtgtat gaccacgcaa ctgtgccagt
    22501 gccatcgcat gcagatgagc ggcagccata tccgctgttg aagcaggcaa gtggtgctgg
    22561 cgcaaacggg taagaaccgt tttcagcagt tgttcacctg cctgctgcaa gccccaccgc
    22621 cagcaccagt tttgccagac cggggcaggc ataccggaaa ggtatcctgc cagcacatca
    22681 aggcgctttt cactgtaggg ggtgagataa caacctgtaa cggcatctgc cagcgagggc
    22741 aattccggcg tgttaatttc ctgcgggcat tcgcgccaca tttttgccag tgcagaagcg
    22801 tgccagccac cgcagacgac taacacgtcg ccattgtttt gctgcatcgc ccaaccaatc
    22861 cagcgggcca taaagtcttc gcgctgacga ttgagagcat ctccggggga atcgccccgc
    22921 agttgtgcaa aatagtgcgc cagtgcagag ggtaatgcag tttgctggct ttcatcttcg
    22981 aacaagtgat cccacagggt gtcgctgtta tccatgcggg tggcacgcag cagcaaggtc
    23041 tggctttcct cttgcgtatc aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc
    23101 aaatcgatga agtaagtttg tgcctgaata cgacgagcgg cttgtagcgc ctgccactcc
    23161 ggcgaaaatt cagcatatgg cgtccaggca ccacgcccgg gggctgcacc gtcctgatac
    23221 tggcaataac tgtaaatagc taccggaagc tggtgggcta aaaacagttc gtctacccgg
    23281 tcattaaaat cagccgggcc ttcaatcaac acgtatcgtg gccgctggct ttcgattaac
    23341 gatttcacca gccgggcgca ggccggacta tgatgccgga tgccgacaat taacggctcg
    23401 ctcatgcatc tttcctcacg gcaggcggtg gcgagcttga taataagcct gccagtgagc
    23461 ttctttatgt gtggcgactc gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc
    23521 gttatctttg acgatggttc cggcaataca gtccaccaga tcggctggct cacctgcgcg
    23581 attcgctaaa aaccaggcgc ggacgcccac tgcgtgggcc acgttcacgg cttcagcggt
    23641 ggacataatt gccgtcaggg tatccattga cgttttcttt tcgccgttgg cacgcagatc
    23701 gcggaaggtg cggaccagta actccagtac cgcgtcaggc actttatgcg gaataccgct
    23761 atgggccagc aaacgcgccg aagcactggc gaccagttcc agctcctggg cgaaatccat
    23821 aatcggaaag acggtttcaa aatcgaagcg acgctttagc gcggcactca tctcattgac
    23881 gccgcgatcg cgggtattgg cagtggcaat aatattaaag ccttctcgcg catagagctg
    23941 actggcttca ccggtgagtt ccggcaccgt cattaccctg tcagaaagca tacctaacag
    24001 acagtcttgt acttccagcg gtgtacgcgt tatctcttca aaacggacaa ttttgccgtc
    24061 gcgcattccc tggtaaagcg gggcagggac cagcgcttcc gttgacggac catggttgat
    24121 aagcagggcg tagttccagc cgtacttgat ttgatcttcg gtggtagatg cgccgccctg
    24181 gatggttaac ccggcatcgc cgctaatagc ggttgccagt aattcagaca ggagcgattt
    24241 tgcggtgcca ggttccccca ccaacatcaa accgcgcccg gtagcgagag tgactaacat
    24301 gcgttcgaca gcggagggac tgatcacgac tttacggcta atattttgcg cgctatcgcc
    24361 aaggataaaa gcgcgggccg caggcaaact taactgccaa ccgggcgggc aaggtgcgtg
    24421 atcattttgt tttaattttg ccagttcatc ggcgtataac acagcggcag gcggacgctg
    24481 cagatgatta ttctgcggtg acatatattc atccctgaaa tgtattaatg aaataaacga
    24541 tattttacgc gcaataaatg tttgccgcat tagttgtttg agcgaaaaat atctggaaag
    24601 cagatcacag taaataaaaa aacctgctgg tgaggcaggt tcttttattt actacaatga
    24661 cgctaacttg ctgattgtgc aacatttcgt accaacgaat aaattataac catatccaca
    24721 ctccattgca attcagtgaa tgagatttat aatcctgccc gttgggaggt tgtgccggat
    24781 cggaccaatt ttaagtcctg gctgctggac gggtggtgcc gcaggcggtg ccctgatccg
    24841 gagagcaacg tttcgtacca acatacgtaa accagt
//
LOCUS       DAZKJR010000072        24859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKJR010000072 DAZKJR010000000
VERSION     DAZKJR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.072"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..591
                     /locus_tag="ACHOWS_002914"
     CDS             46..591
                     /locus_tag="ACHOWS_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFQ0048460.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            566..2491
                     /locus_tag="ACHOWS_002915"
     CDS             566..2491
                     /locus_tag="ACHOWS_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFQ0048461.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2488..2694
                     /locus_tag="ACHOWS_002916"
     CDS             2488..2694
                     /locus_tag="ACHOWS_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFQ0048462.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2691..4292
                     /locus_tag="ACHOWS_002917"
     CDS             2691..4292
                     /locus_tag="ACHOWS_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0048463.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMVIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4273..5592
                     /locus_tag="ACHOWS_002918"
     CDS             4273..5592
                     /locus_tag="ACHOWS_002918"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFQ0048464.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5602..5934
                     /locus_tag="ACHOWS_002919"
     CDS             5602..5934
                     /locus_tag="ACHOWS_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFQ0048465.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            5990..7015
                     /locus_tag="ACHOWS_002920"
     CDS             5990..7015
                     /locus_tag="ACHOWS_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFQ0048466.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            7057..7452
                     /locus_tag="ACHOWS_002921"
     CDS             7057..7452
                     /locus_tag="ACHOWS_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFQ0048467.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            7464..7817
                     /locus_tag="ACHOWS_002922"
     CDS             7464..7817
                     /locus_tag="ACHOWS_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFQ0048468.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7829..8407
                     /locus_tag="ACHOWS_002923"
     CDS             7829..8407
                     /locus_tag="ACHOWS_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0048469.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRISGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8404..8799
                     /locus_tag="ACHOWS_002924"
     CDS             8404..8799
                     /locus_tag="ACHOWS_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFQ0048470.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8807..9547
                     /locus_tag="ACHOWS_002925"
     CDS             8807..9547
                     /locus_tag="ACHOWS_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0048471.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            9563..9985
                     /locus_tag="ACHOWS_002926"
     CDS             9563..9985
                     /locus_tag="ACHOWS_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFQ0048472.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            9967..10401
                     /locus_tag="ACHOWS_002927"
     CDS             9967..10401
                     /locus_tag="ACHOWS_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFQ0048473.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10394..12973
                     /locus_tag="ACHOWS_002928"
     CDS             10394..12973
                     /locus_tag="ACHOWS_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0048474.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            12970..13299
                     /locus_tag="ACHOWS_002929"
     CDS             12970..13299
                     /locus_tag="ACHOWS_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0048475.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13299..13997
                     /locus_tag="ACHOWS_002930"
     CDS             13299..13997
                     /locus_tag="ACHOWS_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFQ0048476.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14003..14746
                     /locus_tag="ACHOWS_002931"
     CDS             14003..14746
                     /locus_tag="ACHOWS_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0048477.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14743..15315
                     /locus_tag="ACHOWS_002932"
     CDS             14743..15315
                     /locus_tag="ACHOWS_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFQ0048478.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15375..18872
                     /locus_tag="ACHOWS_002933"
     CDS             15375..18872
                     /locus_tag="ACHOWS_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFQ0048479.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITW
                     TITSANIDRLRVTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPIWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            18943..19542
                     /locus_tag="ACHOWS_002934"
     CDS             18943..19542
                     /locus_tag="ACHOWS_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFQ0048480.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19607..22633
                     /locus_tag="ACHOWS_002935"
     CDS             19607..22633
                     /locus_tag="ACHOWS_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0048481.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLASGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYVLMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            22630..23532
                     /locus_tag="ACHOWS_002936"
     CDS             22630..23532
                     /locus_tag="ACHOWS_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFQ0048482.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITTFFGSQLQARVQNHILREYLGEQPVGSAFVIETGHNYHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKNATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            23541..24116
                     /locus_tag="ACHOWS_002937"
     CDS             23541..24116
                     /locus_tag="ACHOWS_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFQ0048483.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(24214..24804)
                     /locus_tag="ACHOWS_002938"
     CDS             complement(24214..24804)
                     /locus_tag="ACHOWS_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0048484.1"
                     /translation="MSRIYAYCRVSTLEKTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT         5890 a         6277 c         7570 g         5122 t
ORIGIN      
        1 ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga agtcaacaaa
       61 aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg gcaggaacag
      121 ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga ctctgccgcc
      181 gtcataaaat ggtatgccga aagggatgct gaaattgaga acgaaaagct gcgccgggaa
      241 gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat tgagtacgaa
      301 cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc cagagactcc
      361 gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc aggtgaaatt
      421 gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga actggaaaac
      481 cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc agccgcgctg
      541 gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta acaggctgcg
      601 gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga ccgccgttga
      661 atgggcggat gccaattact atctcccaaa agaatccgca taccaggaag ggcgctggga
      721 aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca tccgtgaggt
      781 gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg tttatgccta
      841 ctttatagag cataagcagc gcaacaccct tatctggttg ccgacggatg gtgatgccga
      901 gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc tgctggcgct
      961 ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc gtttttccaa
     1021 tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg aaaagtcggt
     1081 ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac aggaaggctc
     1141 tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt ccatccgtgg
     1201 ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg aatccccgca
     1261 ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc ttaaatttgg
     1321 cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca gcgtgtttta
     1381 tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta ctgatgcccg
     1441 ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt tttcgtcatc
     1501 cggtgaagag attgagccgc cggacagtgt gacctttcac atctggacgg cgtacagccc
     1561 gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaag acgaaagggg atacgggaaa
     1621 acgtaaaacc ttcgtgaaca ccacgctcgg tgagacatgg gaagcgaaaa ttggcgaacg
     1681 tccggatgct gaggtgatgg cggagcggaa agagcattat tcagcgcccg ttcctgaccg
     1741 tgtggcttac ctgaccgccg gtatcgactc ccagctggac cgctacgaaa tgcgcgtatg
     1801 gggatggggg ccgggtgagg aaagctggct gattgaccgg cagattatta tgggccgcca
     1861 cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct atacccgccg
     1921 gaatggtgca gaaatgtcgg tatcccgtat ctgctgggat actggcggga ttgacccgac
     1981 cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca ttaaaggggc
     2041 atccgtctac ggaaagccag tggccagcat gccacgtaag cgaaacaaaa acggggttta
     2101 ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct tcacactgac
     2161 gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc cggatatttt
     2221 tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat gggtggatgg
     2281 caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg actgcttcgt
     2341 ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc tcagtgcact
     2401 gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac tggcagatta
     2461 cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc cgctgcccgt
     2521 gcggcactgc atgacctgat gacaggaaaa cgggtggcaa cggtacagaa agacggacgg
     2581 cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc ggagctggaa
     2641 gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt atgaaaacgt
     2701 cctccattcc cacccttctg gggccggacg gcatgacatc gctgcgtgaa tatgccggtt
     2761 atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca ccgagtgaaa
     2821 gtgtggatgc agccctgttg cccaacttta cccgtggcaa tgcccgcgcg gacgatctgg
     2881 tacgcaataa cggctatgcc gccaacgcca tccagctgca tcaggatcat atcgtcgggt
     2941 cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag
     3001 cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct
     3061 gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca
     3121 tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc
     3181 ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg
     3241 gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat
     3301 attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc
     3361 gtgagttacc cggcgggcgt gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc
     3421 agacccgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg ctcgacacgc
     3481 tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc accattgaga
     3541 gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt caggagcagc
     3601 gggaaaggct gaccggctgg attggtgaaa ttgccgcgta ttacgccgca gcaccggtcc
     3661 gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac ctgcagacgg
     3721 ctcaggacac ggataacggc tactccgtgt ttgaacagtc actgttgcgg tatatcgctg
     3781 ccgggctggg tgtctcgtat gagcagcttt cccggaatta cgcccagatg agctactcca
     3841 cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga aaattcgtcg
     3901 catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc gttcgccgcg
     3961 tggtgacgtt accttcaaaa gcgcgtttca gctttcagga agcccgcagc gcctggggga
     4021 actgtgactg gataggctcc ggtcgtatgg tcatcgatgg tctgaaagaa gtacaggaag
     4081 cggtgatgct gatagaagcc ggactgagta cctacgagaa agagtgcgca aaacgcggtg
     4141 acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc cgtgcagccg
     4201 gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cggactgcga caatcaacag
     4261 aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag catggctttt
     4321 aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc gcttgcaggc
     4381 cagcttggga tcagtcgcct gacggatgca gtatccggcg acagcctgac tgccggagag
     4441 gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc ccgcagttat
     4501 caggtcatga acggcatcgc cgtgctgccg gtgtccggta cgctggtcag ccggacgcgg
     4561 gcgctgcagc cgtattcggg aatgaccggt tacaacggca ttatcgcccg tctgcaacag
     4621 gctgccagcg atccgatggt ggacggcatt ctgctcgata tggacacacc gggcgggatg
     4681 gtggcgggag catttgactg tgctgacatc atcgcccgtg tgcgtgacat aaagccggta
     4741 tgggcgctgg ccaacgacat gaactgcagt gcaggtcagc tgcttgccag cgccgcctcc
     4801 cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat ggctcacagt
     4861 aattacggcg ctgcgctgga gaaacagggc gtggaaatca cgctgattta cagcggcagc
     4921 cataaggtgg atggcaaccc ctacagccat ctaccgggtg atgtccggga gacactgcag
     4981 tcccggatgg atgcaacccg ccggatgttt gcgcagaagg tgtcggcata taccggcctg
     5041 tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga ggccattgat
     5101 gccggactgg ctgatgaact tgtcaacagc accgatgcga tcaccgttat gcgtgatgca
     5161 ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac tcaatcaaca
     5221 actgtttcag ccactgcttc gcaggctgac gttactgacg tggtgcaagc gacggagggc
     5281 gaaaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgctgc ggttgcggca
     5341 gaaaacagcc gcattatggg gatcctcaac tgtgaggaag ctcacggacg cgaagaacag
     5401 gcacgtgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg cattctggca
     5461 gctgcaccac agagtgcaca ggcgcgcagt gacactgcgc tggatcgtct gatgcagggg
     5521 gcaccggcac cactggctgc aggtaacccg gcatctgatg ccgttaacga tttgctgaac
     5581 acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta ccagccgctg
     5641 ggcaacagtg acccggcaca tacggcaacc gcgcccggcg gattgagtgc gaaagcgcct
     5701 gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg ggatggcacc
     5761 accgacggta ctgccgttgg cattctggcg gttgatgctg accagaccag caccacgctg
     5821 acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga ggctgccagc
     5881 gacgagacga aaaaacggac cgcgtttgcc ggaacggcaa tcagcatcgt ttaaccttac
     5941 ccttcatcac taaaggccgc ctgtgcggct ttttttacgg gattttttta tgtcgatgta
     6001 cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc cgctgtttct
     6061 gcgtctcttt ttccgtgaga gctatccctt caccacggag aaagtctatc tctcacaaat
     6121 tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg aggttatccg
     6181 ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca agcatgaggt
     6241 gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc tggcggaccc
     6301 ggcttaccgc cgccgtcgca tcatcatgca gaacatgcga gacgaagagc tggccattgc
     6361 tcaggtcgaa gagatgcagg cagtttctgc tgtgcttaag ggcaaataca ccatgaccgg
     6421 tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca acatcacaca
     6481 gtccggtggt acggagtgga gcaagcgtga caagtccacg tatgacccga ccgacgatat
     6541 cgaagcctat gcgctgaacg ccagcggcgt ggtgaatatc atcgtgtttg atccgaaagg
     6601 ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc gtcgcggctc
     6661 tcattccgag ctggagacag cggtaaaaga cctgggcgaa gcggtgtcct ataaggggat
     6721 gtatggcgat acggcgatcg tcgtgtattc cggacagtac gtggaaaacg acgtcaaaaa
     6781 gaacttcctg ccggacaaca cgatggtgct ggggaacact caggcacgcg gtctgcgcac
     6841 ctatggctgc attcaggatg cggacgcaca gcgcgaaggt attaacgcct ctgcccgcta
     6901 cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga ttcagtcagc
     6961 accgctgatg ctgctggctg atcctgatgc gttcgtgtcc gtacaactgg cgtaatcatg
     7021 gcccttcggg gccattttct ctctgtggag gagtccatga cgaaagatga actgattgcc
     7081 cgtcttcagg tgctgggtga gcaactgaac cgtgatgtca gcctgacggg gacgaaagaa
     7141 gaactggtgc tccgtgtggc agagctggaa gaggagcttg atgacacgga tgacgctgcc
     7201 ggtcaggaca catctgtcag cccggaaaat gcgctgaccg gacatgaaaa tgaggtggta
     7261 tcagcgcagc cggataccgt gattgatacg gctgctctgg tcacggtcgt ggcactggtg
     7321 acgctgcata ctgatgcact tcacgccacg cgggatgagc ctgtggcatt tgtgctgccg
     7381 ggaacggcgt ttcgtgtctc tgccggtgtg gcagccgaaa tgacagaaca tggcctggcc
     7441 agaatgcaat aacgggaggc gctgtggctg attccgataa cctgttcgat gctgccattg
     7501 cccgcgccga tgaaacgata cgcgggtaca tgggaacgtc agccaccatg acatccggtg
     7561 agctgtccgg tgctgtgata cgtggtgttt ttgatgaccc tgaaaatatc agctatgccg
     7621 gacagggggt gcgcgttgaa ggctccagcc cgtccctgtt tgtccggact gatgatgtgc
     7681 ggcagctgcg gcgtggagac acactgacca tcggcgagga aaacttctgg gtggaccgga
     7741 tttcgccgga tgatggcgga agctgtcatc tctggcttgg gcgtggcgtg ccgcctgccg
     7801 ttaaccgtcg ccgctgaaag ggggatgtat ggccataaaa ggtcttgagc aggccgttga
     7861 aaacctcagc cgtatcagca aaacggcggt gcctggtgcc gccgcaatgg ccattaaccg
     7921 cgttgcgtca tccgcgatat cgcagtctgc gtcacaggtt gcccgtgaga caaaggtacg
     7981 ccggaaactg gtaaaggaaa gggccaggct gaaaagggcc acggtcaaaa atccgcaggc
     8041 cagaatcagg gttaaccggg gggatttgcc cgtaatcaag ctgggtaacg cgcgggttgt
     8101 cctttcccgc cgcagacgtc gtaaaaaggg gcagcgttca tccctgaaag gtggcggcag
     8161 cgtgcttgtg gtgggaaacc gtcgtatttc cggcgcgttt attcagcaac tgaaaaatgg
     8221 ccggtggcat gtcatgcagc gtgtggccgg gaaaaaccgt taccccattg atgtggtgaa
     8281 aatcccgatg gcggtgccgc tgaccacggc gtttaaacag aatattgaac ggatacggcg
     8341 tgaacgtctt ccgaaagagc tgggctatgc gctgcagcat caactgagaa tggtaataaa
     8401 gcgatgaaac atactgaact ccgtgcagcc gtactggatg cactggagaa gcatgacacc
     8461 ggggcgacgt tttttgatgg tcgccccgct gtttttgatg aggcggattt tccggcagtt
     8521 gccgtttatc tcaccggcgc tgaatacacg ggcgaagagc tggacagcga tacatggcag
     8581 gcggagctgc atattgaagt tttcctgcct gctcaggtgc cggattcaga gctggatgcg
     8641 tggatggagt cccggattta tccggtgatg agtgatatcc cggcactgtc agatttgatc
     8701 accagtatgg tggccagcgg ctatgactac cggcgcgacg atgatgcggg cctgtggagt
     8761 tcagccgatc tgacttatgt cattacctat gaaatgtgag gacgctatgc ctgtaccaaa
     8821 tcctgtaatg ccggtgaaag gtgccgggac caccctgtgg gtttataagg gaagcggtga
     8881 cccttatgcg aatccgcttt cagacgttga ctggtcgcgt ctggcaaaag ttaaagacct
     8941 gacgcccggc gaactgaccg ctgagtccta tgacgacagc tatctcgatg atgaagatgc
     9001 agactggact gcgaccgggc aggggcagaa atctgccgga gataccagct tcacgctggc
     9061 gtggatgccc ggagagcagg ggcagcaggc gctgctggcg tggtttaatg aaggggatac
     9121 ccgagcctat aaaatccgct tcccgaacgg cacggtcgat gtgttccgcg gctgggtcag
     9181 cagtatcggt aaggcggtga cggcgaagga agtgatcacc cgcacggtga aagtcaccaa
     9241 cgtgggccgt ccgtcgatgg cagaagatcg cagcacggtg acggcgacaa ccggcatgac
     9301 tgtgacgcct gccagcacct cggtggtgaa agggcagagc accacgctga ccgtggcatt
     9361 ccagccggaa ggcgcaaccg acaagagctt ccgtgcggtg tctgcggata aaacaaaagc
     9421 caccgtgtcg gtcagtggta tgaccatcac cgtgaaaggt gttgctgcag gcaaggtcaa
     9481 cattccggtc gtatccggta atggtgaact tgctgcggtt gcagaaatca ccgtcaccgc
     9541 cagttaatcc ggggagtcag cgatgttcct gaaaaccgaa tcatttgaac ataacggtgt
     9601 gaccgtcacg ctttctgaac tgtcagccct gcagcgaatt gagcatctcg ccctgatgaa
     9661 acggcaggca gaacaggcgg agtcagacag caaccggaag tttactgtgg aagacgccat
     9721 cagaaccggc gcgtttctgg tggcgatgtc cctgtggcat aaccatccgc agaagacgca
     9781 gatgccgtcc atgaatgaag ccgttaaaca gattgagcag gaagtgctta ccacctggcc
     9841 cacggaggca atttctcatg ctgaaaacgt ggtgtaccgg ctgtctggta tgtatgagtt
     9901 tgttgtgaat aatgcccctg aacagacaga ggacgccggg cccgcagagc ctgtttctgc
     9961 gggaaagtgt tcgacggtga gctgagtttt gccctgaaac tggcgcgaga gatggggcga
    10021 cccgactggc gtgccatgct tgccgggatg tcatccacgg agtatgccga ctggcaccgc
    10081 ttttacagta cccattattt tcatgatgtt ctgctggata tgcacttttc cgggctgacg
    10141 tacaccgtgc tcagcctgtt tttcagcgat ccggatatgc atccgctgga tttcagtctg
    10201 ctgaaccggc gtgaggctga cgaagagcct gaagatgatg tgctgatgca gaaagcggca
    10261 gggcttgccg gaggcgtccg ctttggcccg gacgggaatg aagttatccc cgcttccccg
    10321 gatgtggcgg acatgacgga ggatgacgta atgctgatga cagtatcaga agggatcgca
    10381 ggaggagtcc ggtatggctg aaccggtagg cgatctggtc gttgatttaa gtctggatgc
    10441 ggccagattt gacgagcaga tggccagagt caggcgtcat ttttccggta cggaaactga
    10501 tgcgaaaaaa acagcggcag tcgttgaaca gtcgctgagc cgacaggcgc tggctgcaca
    10561 gaaagcgggg atttccgtcg ggcagtataa agccgccatg cgtatgctgc ctgcacagtt
    10621 caccgacgtg gccacgcagc ttgcaggcgg gcaaagcccg tggctgatcc tgctgcaaca
    10681 ggggggtcag gttaaggact ccttcggcgg gatgatcccc atgttcaggg ggcttgccgg
    10741 tgcgatcacc ctgccgatgg tgggggccac ctcgctggcg gtggcgaccg gtgcgctggc
    10801 gtatgcctgg tatcagggca actcaaccct gtccgatttc aacaaaacgc tggtcctttc
    10861 cggcaatcag gcgggactga cggcagatcg tatgctggtc ctgtccagag ccgggcaggc
    10921 ggcagggctg acgtttaacc agaccagcga gtcactgacg gcgctggtga atgccggtgt
    10981 gcgtggtggt gagcagtttg aggcgatcag ccagagtgtg gcgcgtttct cctctgcatc
    11041 cggcgtggag gtggacaagg tcgctgaagc cttcgggaag ctgaccacag acccgacgtc
    11101 gggactgaca gcgatggcac gtcagttcca taacgtgacg gcggagcaga ttgcgtatgt
    11161 tgctcagttg cagcgttccg gagatgaagc cggggcattg caggcggcga acgaggccgc
    11221 aacgaaaggg tttgatgacc agacccgccg cctgaaagag aacatgggca cgctggagac
    11281 ctgggcagac aggacagcac gggcattcaa atccatgtgg gattcggtgc tggatattgg
    11341 tcgcccggac actgcccagg gaatgctgga gaaagcagaa aaggcttttg atgaggcgga
    11401 caaaaaatgg cagtggtatc agagccggag ccaccggcgc ggtaaaacct cagcatttct
    11461 tgccaatctc cggggagcat gggaggacag agcgaatgcg caacttgggc tttcagccgc
    11521 cacgttgcag gccgatcttg aaaaggccag agagatggca gcaaaggact gggccgagtc
    11581 tgaggcatca cggctgaaat ataccgaaga ggcgcagaag gcttacgaac ggctgcagac
    11641 gccgctggag aaatataccg cccgtcagga agaactgaac aaggcactga aagacgggaa
    11701 aatcctgcag gcggattaca acacgctgat ggcggcggcg aaaaaggatt atgaagcgac
    11761 gctgaaaaag ccgaaacagt ccggcgtgaa ggtgtctgcg ggcgatcgtc aggaagacag
    11821 tgctcatgct gccctgctga cgcttcaggc tgaactccgg acgctggaga agcatgccgg
    11881 agcaaatgag aaaatcagcc agcagcgccg ggatttgtgg aaggcggaga gccagttcgc
    11941 ggtactggag gaggcggcgc aacgtcgcca gctgtctgca caggagaaat ccctgctggc
    12001 gcataaagat gagacgctgg agtacaaacg ccagctggct gcacttggcg acaaggttac
    12061 gtatcaggag cgcctgaacg cgctggcgca gcaggcggat aaattcgcac agcagcaacg
    12121 ggcaaaacgg gccgccattg atgcgaaaag ccgggggctg actgaccggc aggcagaacg
    12181 ggaagccacg gaacagcgcc tgaaggaaca gtatggcgat aatccgctgg cgctgaataa
    12241 cgtcatgtca gagcagaaaa agacctgggc ggctgaagac cagcttcgcg ggagctggat
    12301 ggcaggcctg aagtccggct ggagtgagtg ggaagagagc gccacggaca gtatgtcgca
    12361 ggtaaaaagt gcagccacgc agacctttga tggtattgca cagaatatgg cggcgatgct
    12421 gaccggcagt gagcagaact ggcgcagctt cacccgttcc gtgctgtcca tgatgacaga
    12481 aattctgctt aagcaggcaa tggtggggat tgtcgggagt atcggcagcg ccattggcgg
    12541 ggctgttggt ggcggcgcat ccgcgtcagg cggtacagcc attcaggccg ctgcggcgaa
    12601 attccatttt gcaaccggag gatttacggg aaccggcggc aaatatgagc cagcggggat
    12661 tgttcaccgt ggtgaatttg tcttcacgaa ggaggcaacc agccggattg gcgtggggaa
    12721 tctttaccgg ctgatgcgcg gctatgccac cggcggttat gtcggtacac cgggcagcat
    12781 ggcagacagc cggtcgcagg cgtccgggac gtttgagcag aataaccatg tggtgattaa
    12841 caacgacggc acgaacggtc agataggtcc ggctgctctg aaggcggtgt atgacatggc
    12901 ccgcaagggt gcccgtgatg aaattcagac acagatgcgt gatggtggcc tgttctccgg
    12961 aggtggacga tgaagacctt ccgctggaaa gtgaaacccg gtatggatgt ggcttcggtc
    13021 ccttctgtaa gaaaggtgcg ctttggtgat ggctattctc agcgagcgcc tgccgggctg
    13081 aatgccaacc tgaaaacgta cagcgtgacg ctttctgtcc cccgtgagga ggccacggta
    13141 ctggagtcgt ttctggaaga gcacgggggc tggaaatcct ttctgtggac gccgccttat
    13201 gagtggcggc agataaaggt gacctgcgca aaatggtcgt cgcgggtcag tatgttgcgt
    13261 gttgagttca gcgcagagtt tgaacaggtg gtgaactgat gcaggatatc cggcaggaaa
    13321 cactgaatga atgcacccgt gcggagcagt cggccagcgt ggtgctctgg gaaatcgacc
    13381 tgacagaggt cggtggagaa cgttattttt tctgtaatga gcagaacgaa aaaggtgagc
    13441 cggtcacctg gcaggggcga cagtatcagc cgtatcccat tcaggggagc ggttttgaac
    13501 tgaatggcaa aggcaccagt acgcgcccca cgctgacggt ttctaacctg tacggtatgg
    13561 tcaccgggat ggcggaagat atgcagagtc tggtcggcgg aacggtggtc cggcgtaagg
    13621 tttacgcccg ttttctggat gcggtgaact tcgtcaacgg aaacagtgac gccgatccgg
    13681 agcaggaggt gatcagccgc tggcgcatcg agcagtgcag cgaactgagc gcggtcagtg
    13741 cctcttttgt actgtccacg ccgacggaaa cggacggtgc cgtttttccg ggacgtatca
    13801 tgctggccaa cacctgcacc tggacctatc gcggtgatga gtgcggttat cacggtccgg
    13861 cggtcgcgga tgaatatgat cagccgacgt ccgatatcac gaaggataaa tgcagcaaat
    13921 gcctgagcgg ctgtaagttt cgcaataacg tcggcaactt tggcggcttc ctttccatta
    13981 acaaactttc gcagtgaatc ccatgacaga gacagaatca gcgattctgg cgcacgcccg
    14041 gcgatgtgcg ccagcggagt cgtgcggctt cgtggtgaga acaccggagg gggaaagata
    14101 ttttccctgc gtgaatatct ccggtgagcc ggaggcgtat ttccggatgt cgccggagga
    14161 ctggctgcgg gcagagatgc agggtgagat tgtggcgctg gtccacagcc accccggtgg
    14221 tctgccctgg ctgagtgaga ccgaccggcg gctgcaggtg cagagtgatt tgccgtggtg
    14281 gctggtttgc cggggggcga ttcacaagtt ccgctgtgtg ccacatcttt ccgggcggcg
    14341 ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcttacc atctggcggg
    14401 gattgagatg ccggattttc atcgcgagga tgactggtgg cgtcacggtc agaatctcta
    14461 tctggataat ctggaggcca cagggctgta tcaggtgccg ttgtcatcag cacaaccggg
    14521 cgatgtgctg ctgtgctgtt ttggttcatc ggtgccgaat catgccgcca tttactgtgg
    14581 tgacagcgag ctgctgcacc atattcctga acaactgagc aaacgagaga ggtacaccga
    14641 caaatggcag cgacgcacac actccctctg gcgtcaccgg gaatggcacg catctgcctt
    14701 tacggggatt tgcaacgatt tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa
    14761 gccatccgcg cactggccac acagctcccg gcgtttcgtc agaaactgag cgacggctgg
    14821 tatcaggtac ggattgccgg gcgggacgtc agcacgtccg ggttaacggc gcagttacat
    14881 gagactctgc ctgacggcgc tgtgattcat attgttccca gagtcgccgg ggccaagtca
    14941 gggggcgtat tccagattgt tctgggggct gccgccattg ccggatcatt ctttactgcc
    15001 ggagccaccc ttgcagcatg gggggcagcc attggggccg gtggtatgac cggcatcctg
    15061 ttttctctcg gtgccagtat ggtgctcggt ggtgtggcgc agatgctggc accgaaagcc
    15121 agaactcccc gtacacagac aacggataac ggtaagcaga acacctattt ctcctcactg
    15181 gataacatgg ttgcccaggg caatgttctg cctgttctgt acggggaaat gcgcgtgggg
    15241 tctcgcgtgg tttctcagga gatcagcacg gcagacgaag gggatggtgg tcaggttgtg
    15301 gtgattggtc gctgatgaaa aacgtttatg tgaaaccgcc tccgggcggt tttgtcgttt
    15361 atggagcgtg aggaatgggt aaaggcagca gtaaggggca tactccgcgc gaagcgaagg
    15421 acaacctgaa gtccacgcag ttgctgagtg tgatcgatgc catcagcgaa gggccgattg
    15481 aaggtccggt ggatggatta aaaagcgtgc tgctgaacag tacgccagtg ctggacagtg
    15541 aggggaatac taacatctcc ggcgtcacgg tggtgttccg ggccggtgag caggagcaga
    15601 caccgccgga gggatttgaa tcctccggct ccgagacggt gctgggtacg gaagtgaaat
    15661 acgacacgcc gatcacctgg accatcacgt cggcaaacat tgaccgactg cgtgttacct
    15721 tcggcgtgca ggcactggtg gaaaccacct caaaggggga caggaatccg tcggaagtcc
    15781 gcctgctggt tcagatacag cgtaatggtg gctgggtgac ggaaaaagac atcaccatta
    15841 agggcaaaac cacctcgcag tatctggcct cggtggtggt gggtaacctg ccgccgcgcc
    15901 cgttcagtat acggatgcgc aggatgacgc cggacagcac cacagaccag ctgcagaaca
    15961 aaacgctctg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg
    16021 cactggtcgg tgtgcaggtg gactcggagc agttcggcag ccagcaggtg agccgtaatt
    16081 atcatctgcg cgggcgtatt ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat
    16141 acagcggtat ctgggacgga acgtttaaac cggcatacag caacaacatg gcctggtgtc
    16201 tgtgggatat gctgacccat ccgcgctacg gcatggggaa acgtcttggt gcggcggatg
    16261 tggataaatg ggcgctgtat gtcatcggcc agaattgcga ccagtcggtg ccggacggct
    16321 ttggcggcac ggagccgcgc atcacctgta atgcctggct gaccacacag cgcaaggcgt
    16381 gggatgtgct cagtgatttc tgctcggcga tgcgctgtat gccgatatgg aacgggcaga
    16441 cgctgacgtt cgtgcaggac cgaccatcag ataaggtgtg gacctataac cgcagtaatg
    16501 tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg aaggaccgcc
    16561 ataatgccgt tgaggtgaac tggattgacc cggataacgg ctgggagacg gcgacagagc
    16621 ttgtggagga cacgcaggcc attgcccgtt acggtcgtaa cgtcacgaag atggatgcct
    16681 ttggctgtac cagtcggggg caggcgcacc gcgccgggct gtggctgatt aaaacggaac
    16741 tgctggaaac gcagaccgtg gacttcagcg tgggtgcgga agggcttcgc catgtaccgg
    16801 gggatgtcat tgaaatctgc gatgatgact atgcgggtat cagcaccggc gggcgcgtgc
    16861 tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg ctgccatcct
    16921 ccggtaccac gctgataagc ctggttgacg gaagtggcaa tccggtcagc gtggaggtcc
    16981 agtccgtcac cgacggcgtg aaggtaaaag tgagccgtgt tcctgacggc gttgccggat
    17041 acagcgtgtg ggggctgaag ctgccgacgc tgcgccagcg actgttccgc tgcgtgagta
    17101 tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg ccggaaaaag
    17161 aggccatcgt ggataacggg gcgcactttg acggcgacca gagcggcacg gtgaatggtg
    17221 tcacgccgcc agcggtgcag cacctgactg ccgaagtcac cgcagacagc ggggaatatc
    17281 aggtgctggc gcgctgggac acaccgaagg tggtgaaggg cgtgagcttc ctgctccgtc
    17341 tgaccgtaac agcggacgac ggcagtgagc ggctggtcag cacggcccgg acgacggaaa
    17401 ccacataccg cttcacacaa ctggcgttgg ggaactacag gctgacagtc cgggcggtaa
    17461 atgcgtgggg acagcagggc gatccggcat cggtatcgtt ccggattgcc gcaccggcag
    17521 cgccgtctcg gattgagctg acaccgggct attttcagat aaccgccacg ccgcatcttg
    17581 cggtttatga cccgacggta cagtttgagt tctggttctc ggaaaaacgg attgcggata
    17641 tcaggcaggt tgaaaccagc gcgcgttatc ttggtacggc actgtactgg atagccgcca
    17701 gtatcaatat caaaccgggc catgattatt atttttacgt tcgcagtgtg aacaccgttg
    17761 gcaaatcggc attcgtggag gctgtcggtc agccgagtga tgatgcatca ggctatctgg
    17821 attttttcaa aggcgagata gggaaaaccc atctggctca ggagctgtgg acgcagattg
    17881 ataacggtca gcttgcgcct gacctgactg aaatcaggac gtccataacg gatgtcagca
    17941 atgaaataac acagaccgtc aataagaaac tggaagacca gagtgcagcg atccagcaga
    18001 tacagaaggt tcaggttgat acaaataata acctgaacag catgtgggca gtgaagctgc
    18061 agcagatgca ggacggacgc ctttatattg cgggtatcgg tgccggtatt gagaacaccc
    18121 ctgacggcat gcagagtcag gtgctgctgg cagcagacag gattgcgatg attaatcctg
    18181 cgaatggcaa cacaaagccg atgtttgttg gtcagggcga tcagatattc atgaatgaag
    18241 tgttcctgaa acgcctgacg gcccccacca ttaccagcgg cggtaatcct ccggcatttt
    18301 ccctgacatc agacgggaga ctgacggcga aaaatgcgga tatcagtggc agtgtgaatg
    18361 cgaactcagg aacgctcaac aatgtcacga ttaaccagaa ctgtacgatt aagggcatgc
    18421 tggaggcgac ccaggtcaga ggagatttcg ttaaagctgt atcaaaagcc ttcccgaaaa
    18481 aagtcggtac gtggggtaac acggaaacac caaacggtac ggttacagtc accatcagcg
    18541 atgatcataa ctttgaccgc cagattatta ttccgcccat tatttttaac ggtatagcgt
    18601 atgacgatcc ggggagcgga aataacccag gaggcacgcg atacacgggt tatggttttg
    18661 aagttcgcaa aaacggcgta ttaatcgcat ccagagaaac taaaggggcc attcccggta
    18721 gttacagtgc agttattgat atgccgagtg gcaggggaag cgtcactctg gagtttaaga
    18781 ttttccagaa aggcaatcag ggggcaggca atatcaccga ctgtacggtg attgtgacca
    18841 aaaaagccgc ttccggcatc agtattcgtt gaaatattta taaccccaat aaagggcgtc
    18901 aggaatgacg ccttttttat tgcagaaaag cgagaggtaa ttatgcgtaa agtttgtgca
    18961 gcaattttgt ccgcagccat ttgtctggcc gtatccggtg cgcctgcatg ggcgtctgaa
    19021 catcagtcca cgctgagcgc ggggtatctt catgcctcga cgaacgtccc cggcagcgat
    19081 gatctgaacg ggattaacgt gaaataccgt tatgagttta cggacacact ggggctggtg
    19141 acgtcattca gctatgcagg agacaagaat cgccagctga cccgttacag cgatacccgc
    19201 tggcatgaag attccgttcg taaccgctgg ttcagcgtaa tggcggggcc gtctgtgcgc
    19261 gtgaatgaat ggttcagcgc gtatgcgatg gcgggtgtag cttacagccg tgtgtcgact
    19321 ttctccgggg attatcttcg cgtaactgac aacaagggga aaaagcacga tgtgctgacc
    19381 ggaagtgatg acggtcgcca cagcaacacg tctctggcgt ggggggctgg cgtgcagttt
    19441 aacccgaccg aatccgtggc cattgatatt gcttatgaag gctccggcag tggtgactgg
    19501 cgcactgacg gtttcatcgt gggtgtcggt tataaattct gattagccag gtaacacagt
    19561 gttatgacag cccgccggtt caggcgggct tttttgtggg gtgaatatgg cagtaaagat
    19621 ttcaggtgta ctgaaagacg gcacaggaaa accggtacag aactgcacaa tccagctgaa
    19681 agcaaaacgt aacagcacca cggtggtggt gaacacgctg gcctcagaaa atccggatga
    19741 agccgggcgt tacagcatgg acgttgagta cgggcagtac agcgttattc tgttggtgga
    19801 gggattcccg ccgtcacatg ccgggaccat caccgtgtat gaagattccc gacccggtac
    19861 gctgaatgat tttctcggtg ccatgacgga ggatgatgcc cgtcctgagg cactgcgccg
    19921 ttttgaactg atggtggaag aggtggcgcg taacgcgtcc gcggtggcac agaacacggc
    19981 agccgcgaag aagtcagcca gcgatgccag cacatcagcc cgtgaggcgg caacccatgc
    20041 gactgatgct gcagattcag cacgtgcagc cagcacgtca gccggacagg ccgcttcgtc
    20101 ggctcagtca gcgtcttcca gcgcaggaac ggcatcaaca aaggccactg aagcctcaaa
    20161 aagtgctgcc gctgcagagt cctcaaaaag cgtggcagct accagtgccg gtgcggcgaa
    20221 aacgtcagaa acgaatgccg cagcgtcaga acaatcagcc gccacttctg catccaccgc
    20281 gaccacgaaa gcgtcagaag ctgccacctc agcccgggat gcggcggcct caaaagaggc
    20341 agcgaaatca tcagaaacga acgcatcctt gagcgccagt agcgcagctt cctcggcaac
    20401 ggcggcagga aattccgcga aggcggcaaa aacgtccgag acgaacgcca ggtcttctga
    20461 gacggcagcg ggacagagcg cctcagctgc ggcaggctca aaaacagcgg ctgcgtcgtc
    20521 tgccagtgcc gcgtcaacaa gtgccgggca ggcctcagcc agtgccaccg ccgccggaaa
    20581 atcggcagaa agcgccgcat catccgcttc aacagccaca acgaaggctg gcgaagccac
    20641 tgagcaggcc acggcagcag cgaggtcagc ttccgcagcg aagacatccg aaacgaacgc
    20701 gaaagcgtcg gaaacccgtg cagaatcctc aaaaacggca gcggcatcgt ccgccagttc
    20761 ggcggcgtca tcggcatcat cggcgtctgc ttcaaaagat gaggcgacca gacaggcgtc
    20821 agcagcgaag ggcagcgcca cgacggcatc cacgaaggcg acagaggctg ctggtagtgc
    20881 gaaggcagca gctcagagca aaagtacggc ggaatctgca gcaacgcgcg ctgagacagc
    20941 ggcaaaacgg gcagaggata ttgcatccgc cgtggcgctt gaggatgcga gcacgacgaa
    21001 aaaggggata gtacagctca gtagtgcgac taacagcact tccgagtcac tggcggcaac
    21061 accaaaagcc gttaaggccg cgtatgacct ggctagcggg aaatacaccg cacaggatgc
    21121 aacgacagca cagaaaggga taatccagct aagcagcgcg accaacagca cgtctgaaac
    21181 gctggcggca acgccaaagg cagtaaaagc agccaatgac aatgctgaga aacgtctgca
    21241 gaaagatcag aacggtgcgg atatccctgg caaagacacc tttacgaaaa atattggtgc
    21301 ctgccgtgcc ttcggtgggt cagtaagcac aacaacagga aactggacga ctgcacagtt
    21361 tatcgagtgg ctggattctc agggagcatt taaccatcca tactggatgt gcaagggttc
    21421 ctggtcttat ggcaataata aaatcattac tgatactggc tgcggtaata ttcatctcgc
    21481 cggagctgtc attgaagtaa tggggataaa gtcagcgatg acgatccgca ttaccactcc
    21541 gaccacatcc agcggtggcg gaacaaccaa cgcccagttt acctatatta atcacggaac
    21601 agattattca cctggctggc gaagggacta taactccaga aataagccaa cggcatcaga
    21661 gatcggggcg ctaccgtcag gtggaacagc agtatcatca gttaatctgg cttcaaaagg
    21721 tcgggtagcc gcgctgacag acaatacgca gggggcagca ggtcttgagt tatacgaggt
    21781 gtataacaac ggatatccaa caacgtatgg aaatatcatt cacctgaaag ggatgacagc
    21841 cgttggcgaa ggtgagttac tcatcggctg gagtggtaca agcggtgctc atgctccggc
    21901 atttattcgt tcacgacggg atacgaccga cgcaaactgg tcgccgtggg cgcagcttta
    21961 cacctcggct catcctcctg cagagtttta tccagtcggt gcaccaatcc cgtggccatc
    22021 agataccgtt ccgtctggtt atgtcctgat gcaggggcag acttttgaca aatctgcata
    22081 cccgaaactt gcagccgctt atccgtcagg cgtgatccct gatatgcgtg gctggacgat
    22141 taagggcaaa cctgccagtg gtcgggccgt attgtctcag gaacaggacg gcattaaatc
    22201 gcacacccac agcgccagcg catccagtac ggatttgggg acgaaaacca catcgtcgtt
    22261 tgattacggc actaaatcca cgaataacat cggggcgcat acgcacagtg tgagcggtac
    22321 agccgcaagt gccggaaacc atactcatag tgtcacaggc gcatcagcag tcagccagtg
    22381 gtcacaaaat gggtcagtac ataaggtagt gtctgcggcc agtgtgaata caagtgctgc
    22441 aggagcgcac actcatagtg tcagcggcac agctgcatct gcaggtgctc acgcacatac
    22501 tgtcggtatt ggtgctcata cgcactcggt tgcgattggc tcacatggac acaccatcac
    22561 cgttaacgct gctggtaacg cggaaaacac cgtcaaaaac atcgcattta actatattgt
    22621 gaggcttgca tgattacgct cattctttct gcaccagtaa ccgaaatggc tgaagcattt
    22681 aagcgggtat ttgcaaatgc agataatgtg aatattgtcg gaaagccatt tgaaacaatc
    22741 agagaatttg actgcatggt aagtgcggca aatagtttcg gcctgatgga tggcggtgtt
    22801 gatgccgcca ttaccacatt cttcggttct cagctacagg cccgcgttca gaatcatatt
    22861 cttcgtgaat atctcggcga gcagcctgta ggttctgcat ttgttattga aacggggcat
    22921 aattatcacc cctggctggt acatgcgcca acaatgcgtg ttccgttgac aattgacgga
    22981 acagacgctg tatataacgc aacttgggca gctctacttg ctatctttca gcacaataaa
    23041 aacgcaacga cagacaggaa aataaagacg gtggtattcc ctgcaatggg agccggatgt
    23101 ggtcaggtgc cgtttgaaag cgttgcccgg cagatgaagc aggcatggga taactttaat
    23161 aaaaaaacag aatcaattaa ctgggaatac gcacaatccc gccagtcggc agtatttggc
    23221 acatatgcat actgtccggg taattctgtt tgccgttatg cggatactaa atatattgga
    23281 tgcggcgatt atcggacgta ttgctcacgt tccgggcagg tctgtattaa ccctgaacat
    23341 caggctgatg atgtactgat acaacatcag gctaataacc ggtttcgccc tggttcgcat
    23401 atacaccgga ttaatccaga aaatcctgta ggtaatgtca tctctggcgc acatagccac
    23461 ggaagtagca tcgttattgg tgctcacacc catacgctca ataaacaata ttctgtctct
    23521 gatattaagt agaggtgaac atggatttca gaatgagtga acaaccacgg accataacaa
    23581 tttataatct gctggccgga actaatgaat ttattggtga aggtgacgca tacattccgc
    23641 ctcatacagg tctgccagca aacagtaccg atattgcacc gccagatatt ccggctggtt
    23701 tcgtggctgt tttcaacagt gatgagtcat cgtggcatct cgttgaagat catcggggta
    23761 aaacggttta tgacgtggct tccggcgacg cgttatttat ttctgaactc ggctcattac
    23821 cggaaaatgt cacctggtta tccccggaag gggagtttca gaagtggaac ggcacagcct
    23881 gggtgaagga tacggaagca gaaaaactgt tccggatccg ggaggcggaa gaaacaaaaa
    23941 acagcctgat gcaggtagcc agtgagcata tagcgccgct tcaggatgct gtggatctgg
    24001 agatcgcaac ggagaaagaa accttgttgc tggaagcctg gaaaaagtat cgggtgttgc
    24061 tgaaccgtgt tgatacatca actgcaccgg atattgaatg gccagcccta ccgtagggta
    24121 agatatataa attctataat tagaagtatc tttccattta aggctaggaa ggggggcttg
    24181 gaaaacgtaa ggaatctcac accgagatta tttttatata tcaggtgtct gattttttgc
    24241 tttagctctt aaaatggttt gccgcgaggt tttgaattct tgggcaatgt cacttatatt
    24301 tatacctgac ttaatttgct ctagtaccct ctgtatttgt tcatcattta acacaggtgg
    24361 gcgaccaaat cgctttcctg cgccccgtgc tcttaatatc cctgaatgag tacgctcaag
    24421 taaaaggtct cgctcaaatt cagcgactgc tgaaattact tgcatcatca tttttccagg
    24481 tggactggtc aggtcaacgc cccccaatgc taagcaatgc actttgatac ctgctttggt
    24541 cagttgttcc accgttttac tgatatccat tggattacaa ccgaggcgat ccagttttgt
    24601 gacaatcaat gcatcacctc ttttcagtcg atcaagcaac tggttaaaac caggacgctc
    24661 actggttgct gctgagccgc taatttgttc ttcaattatt tgctgagatt tgatgttaaa
    24721 acctgcactt tcgatttccc gacgttgatt tttggttgtc ttttccagag ttgatacccg
    24781 acagtaagca taaattcgag acatagtgag atcttctata cgaaattggt gtacatatca
    24841 taatgcatct cagaaaata
//
LOCUS       DAZKJR010000073        24669 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKJR010000073 DAZKJR010000000
VERSION     DAZKJR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.073"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..402)
                     /gene="yhaH"
                     /locus_tag="ACHOWS_002939"
     CDS             complement(37..402)
                     /gene="yhaH"
                     /locus_tag="ACHOWS_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFQ0048485.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="ACHOWS_002940"
     CDS             complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="ACHOWS_002940"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFQ0048486.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFNHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1749..2141)
                     /gene="yqjF"
                     /locus_tag="ACHOWS_002941"
     CDS             complement(1749..2141)
                     /gene="yqjF"
                     /locus_tag="ACHOWS_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFQ0048487.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2328..2627)
                     /gene="yqjK"
                     /locus_tag="ACHOWS_002942"
     CDS             complement(2328..2627)
                     /gene="yqjK"
                     /locus_tag="ACHOWS_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFQ0048488.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2617..3021)
                     /gene="yqjE"
                     /locus_tag="ACHOWS_002943"
     CDS             complement(2617..3021)
                     /gene="yqjE"
                     /locus_tag="ACHOWS_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0048489.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3024..3329)
                     /gene="yqjD"
                     /locus_tag="ACHOWS_002944"
     CDS             complement(3024..3329)
                     /gene="yqjD"
                     /locus_tag="ACHOWS_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFQ0048490.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3367..3735)
                     /gene="yqjC"
                     /locus_tag="ACHOWS_002945"
     CDS             complement(3367..3735)
                     /gene="yqjC"
                     /locus_tag="ACHOWS_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFQ0048491.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3882..4265)
                     /gene="mzrA"
                     /locus_tag="ACHOWS_002946"
     CDS             complement(3882..4265)
                     /gene="mzrA"
                     /locus_tag="ACHOWS_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFQ0048492.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4269..4931)
                     /gene="yqjA"
                     /locus_tag="ACHOWS_002947"
     CDS             complement(4269..4931)
                     /gene="yqjA"
                     /locus_tag="ACHOWS_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFQ0048493.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4953..5036)
                     /locus_tag="ACHOWS_002948"
     CDS             complement(4953..5036)
                     /locus_tag="ACHOWS_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048494.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            complement(5276..6052)
                     /gene="exuR"
                     /locus_tag="ACHOWS_002949"
     CDS             complement(5276..6052)
                     /gene="exuR"
                     /locus_tag="ACHOWS_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFQ0048495.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQATDNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6182..7480)
                     /gene="exuT"
                     /locus_tag="ACHOWS_002950"
     CDS             complement(6182..7480)
                     /gene="exuT"
                     /locus_tag="ACHOWS_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFQ0048496.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            7963..9375
                     /gene="uxaC"
                     /locus_tag="ACHOWS_002951"
     CDS             7963..9375
                     /gene="uxaC"
                     /locus_tag="ACHOWS_002951"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0048497.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9390..10877
                     /gene="uxaA"
                     /locus_tag="ACHOWS_002952"
     CDS             9390..10877
                     /gene="uxaA"
                     /locus_tag="ACHOWS_002952"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFQ0048498.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10960..11511
                     /gene="ygjV"
                     /locus_tag="ACHOWS_002953"
     CDS             10960..11511
                     /gene="ygjV"
                     /locus_tag="ACHOWS_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFQ0048499.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11516..12760)
                     /gene="sstT"
                     /locus_tag="ACHOWS_002954"
     CDS             complement(11516..12760)
                     /gene="sstT"
                     /locus_tag="ACHOWS_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0048500.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLINDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12965..13267)
                     /locus_tag="ACHOWS_002955"
     CDS             complement(12965..13267)
                     /locus_tag="ACHOWS_002955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048501.1"
                     /translation="MASKSPKRSLEAGATRMDARRGRILQGRSLAPDPVAARDKLREP
                     RSGDFVRQSLGVQGAAAIGRPLRAPCASGFIWHSTLKERNRSHIPNSFAHMLSNQP"
     gene            complement(13376..14341)
                     /gene="alx"
                     /locus_tag="ACHOWS_002956"
     CDS             complement(13376..14341)
                     /gene="alx"
                     /locus_tag="ACHOWS_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFQ0048502.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14624..15610)
                     /gene="ygjR"
                     /locus_tag="ACHOWS_002957"
     CDS             complement(14624..15610)
                     /gene="ygjR"
                     /locus_tag="ACHOWS_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0048503.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKNVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15689..16372)
                     /gene="ygjQ"
                     /locus_tag="ACHOWS_002958"
     CDS             complement(15689..16372)
                     /gene="ygjQ"
                     /locus_tag="ACHOWS_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFQ0048504.1"
                     /translation="MQARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVSAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVGAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            complement(16449..16952)
                     /gene="ygjP"
                     /locus_tag="ACHOWS_002959"
     CDS             complement(16449..16952)
                     /gene="ygjP"
                     /locus_tag="ACHOWS_002959"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFQ0048505.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17037..18173
                     /gene="rlmG"
                     /locus_tag="ACHOWS_002960"
     CDS             17037..18173
                     /gene="rlmG"
                     /locus_tag="ACHOWS_002960"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFQ0048506.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18364..18447
                     /locus_tag="ACHOWS_002961"
                     /pseudo
     CDS             18364..18447
                     /locus_tag="ACHOWS_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            18444..18860
                     /gene="higA"
                     /locus_tag="ACHOWS_002962"
     CDS             18444..18860
                     /gene="higA"
                     /locus_tag="ACHOWS_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFQ0048507.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18905..20923)
                     /gene="fadH"
                     /locus_tag="ACHOWS_002963"
     CDS             complement(18905..20923)
                     /gene="fadH"
                     /locus_tag="ACHOWS_002963"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFQ0048508.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KSHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQQLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(21094..23445)
                     /gene="ygjK"
                     /locus_tag="ACHOWS_002964"
     CDS             complement(21094..23445)
                     /gene="ygjK"
                     /locus_tag="ACHOWS_002964"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0048509.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSATLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNKRNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKAETLSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(23462..24532)
                     /gene="ygjJ"
                     /locus_tag="ACHOWS_002965"
     CDS             complement(23462..24532)
                     /gene="ygjJ"
                     /locus_tag="ACHOWS_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFQ0048510.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVSGSES
                     GMMVSAMYEALKGIYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
BASE COUNT         6018 a         6574 c         6329 g         5748 t
ORIGIN      
        1 gccggatgag tcagacatcc ggcgagtaga tttgttttaa ggttccagtt ttggatcagg
       61 tccaaagcgg ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca
      121 gccgataaac gggattaaga acagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag
      181 gcggcggaac tgaactgccc accacggtaa aaacaccaga ataccgtaga ttgtcgtcag
      241 gatgccttcg ccaccggcac gttgccagcc taacatttta tccagcagcc ccagcacgaa
      301 cgtaaagatg atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg
      361 gaaaccgaca taatttttta gtactttcag ataccagtcc atttttgcct caattttgct
      421 caatcacttg tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata
      481 ttcgttcaag gaattaataa agtgccgtaa agcgcgctaa gactgatgac aagcgcaaaa
      541 tcgccagatg cgctacgctt atctggccta cgcggcattg taacatattg aatataaacg
      601 ttttgtaggt tggagaagac gttcacgccg catccgacat aaataacacg cacttagcta
      661 ataatcagag gcacctcgta aggtgccttt ttttaaccga agcgaacatc tcgtccatgc
      721 ggttcatcga gatcctgcca cggaccaatt gaaataatcc ccgtagggtt gatggtctta
      781 tggctgcgga agtaatgatt acggatgtga ttgaaattga cagtttcggc gatccccggc
      841 atctggtaga tatcgcgcag aaaaccgtac agattcaggt aatcgctgat gcggtgctta
      901 tcacacttga agtgggtcac atacaccgga tcaaaacgga ccagcgtggt ccacaggcga
      961 atatcggctt cggttagctg attgccggtc aggtaacgat gctggcctaa aatctgctcc
     1021 agtcgcgcca gcgattcaaa cactttcgcc accgcctcgt cgtaagcttg ctggctggtg
     1081 gcaaaaccag ctttatacac gccgttgtta acggtgtcat aaatccagcc gttaagttcg
     1141 tcaattttcg tttgcagggc tggtgggtag taatcacccg ctttcgcgcc cagcgcatca
     1201 aacgcggtat tgaacatgcg gatgatttcc gctgattcgt tgctgacgat ggtgtggttc
     1261 tttttgtccc acagtacggg aacagtaact cgtccgctgt agtgtggatc ggcgtggaga
     1321 taaagctgat acaaaaattc atgttgatag agtgtatcgc cggttgcacc cggaaaactg
     1381 tcatcaaagg tccagccgtt ttccagcatc agcgggttca ctacagaaac ggaaataaac
     1441 ggttccagtc ctttgagttt gcgcatgatc agcgtgcggt gcgcccacgg gcaggcgagt
     1501 gaaacataga gatgataacg atctttctct gcgataaaac cgcctgtgcc agtggggcct
     1561 ggcgcgccat cggcagtgag ccagttacga aatgcggaag ctgaacgttg aaatttaccg
     1621 ccggtagatt tggtatcgta ccaggtgtca tgccagacgc cgtcaatcag ttgacccata
     1681 tttctctcct ccaatagcaa aaacgaggag cttcctcctc gttttttatc gttaagtata
     1741 gcgcgtgctt accacttttt attcagcagg cggtcgatgc tatacgcgcc cggaccggta
     1801 attgccagca gcaggaatcc gccagaaatt gtcaggtttt tcatgaacat cagcgagttg
     1861 acgccttcag caaagttgct gtgaaataaa aatgccgtca gcagcgtaaa gcccgcagta
     1921 aacagggctg tggtgcgagt caggaaaccg aacaggattg ccagaccacc accaaactca
     1981 agcagaatca ccagcggcag cataaaaccc gggacgccca tcgcttccat atattgttgg
     2041 gtacccgcgt aaccagtaat ttttccccag cctgcggtaa taaacagaat cggcattaaa
     2101 atgcgcgcta ccagtacacc aacatcttct aattttttca tcatactctc cactgcgccg
     2161 ctcaggctgc tgattgtttt tttgtgcaag ggcgcgctat tagcgtcgtt gctgtcgatg
     2221 gagagaatca taaacgtggc gaagcgtgat tgttagcaag gaaaactgtc aaatattttc
     2281 aaaaaatttg agggataagg ccggaatttc tccggccaga gaggaagtta accgcgaagc
     2341 tgttgttgct tgagggttgt tttcaccaga cgccaggcgc tccatacacc aaaaccgcgt
     2401 ctggcccagc ggaccagcat attaggatgg cgaatcgtcc agatagccat cacgctactg
     2461 ccaaccagcg cccaggagcg cagactcaac agcatattcc agcgacgatc gtaagcgcct
     2521 gttgcctcca gccattcacg acgactggcg gaaagatcca gccgttgttg ctggatctgg
     2581 ctaagcagtt gcgccttacg tcgttcacgt tcgactttac tgctcacggg actcctcctc
     2641 gagcagctgc cgatcgtttg ccagctcatg gcgcgtgtgg cgcagcaacg tagacttacg
     2701 cgatttacgt aacgtccaga tgccgccaat cagtgccagt agcagcaaca ccacggtggt
     2761 ggcaatcatc gcattcaggc gatattgcgg atcaaccgcc cagataatta gcaccatcag
     2821 gctcataaga ccaaatgcag cgaaaagcat cgtcaggccc agcatcagta aaagttgaaa
     2881 gagattcgct ttttcctctt ccagctccac caccgccagc cgcagacgtg tctccaccat
     2941 ttcaaccatg atggaaacaa ttcgctgccc gatgcccaga acgcttttac cgggcccttg
     3001 cgcgtgatga gtgtccgcca taattaacga cgcgacagca gaacaccgag cactacaccg
     3061 attgctgcgc caatgcccac gcccgtccac ggattttcac gcacatactc atcggcacgc
     3121 gccgccgcga cacgggtttg tttggcaatg gcatcaccgg tttcacccag gcgataacgg
     3181 ctctgtttca gtgcctgctc cgctttgcta cgaatcttac tcaactcttc tttcgacttc
     3241 tcgccagatg agctaagcac ctcttccagc gtatcggaaa gggatttcaa ctcagcacgc
     3301 agatgttccg tagtgtgttc tttcgacata gttttctcca ggtgagtaag tagtgactat
     3361 tgtgagttag tagtcgcgcg cttccagctt tttcagctct tcctgcgctt ctgccagttt
     3421 ccgttcgcgt ttggcaatct tatctgcatc gcctttttgc ttcgcctcgg ctaaatcttg
     3481 ctggcgttcc gccacctcat ctttctgctt tgcgattttc ttctgatgat cggcacgcag
     3541 ctggctatct gaacagttgg cccggacttc actcagggct ttattcagac cgtcaatacg
     3601 attctggttt tggtgttttt cggcatagct gatctcctta aggatatttt gctccttttc
     3661 ctgacacagg gtagtggcat aactaccggc actaagagca aagagagaaa cagctaaagc
     3721 gatgcggtat ttcattcttg aaccttccat tgtgattcgg acctattaca aaggaacgat
     3781 tgtccagaca acgaagcggt cgtgttaacc accgcctcat taatcataga cagttacggt
     3841 gaaatcacca aagtgagtga tctgtttcgg ataacaggaa gttatccgaa gcgatgagag
     3901 ttatcccgca accgggtcag ccactgcata gcctgactgt tattgtcctg ctgcgcaatg
     3961 atgtagccat ggggcaatgt tctgtcgagg accgcttttg cggcggcgct ctggtcgctg
     4021 gagtcaaagg taattaacag agtgtcgttt ttgggggtga tacttttgaa aggaatgccg
     4081 tgagcgtcaa ggtgatgcca gattgaaaaa ccgtctggca tcgttgtccc ttgatgaacg
     4141 gcacgaatcg ccagcgtaga ctcttgctgg cgaaccgcgg accaggccag caacagtgtg
     4201 ccgaccagaa gtaaaacagc gccggaccag gctagctggc gaagcgacat gcgaggtatt
     4261 tgcatccctt acccccgatt tccatatttc tttttccata acacgaccag agaacctgcc
     4321 aggccaaaca ccagcagcac caccgggagc agcatcaggc atgacatcag ctggtcctcg
     4381 tactttaaaa ataccggcgt tttgccgagc atgtaaccca gagttgtcag gatcaatacc
     4441 cacaacaaac cactcatcca gttgaagaac tgaaagcgcg cgttattcag ccctgataac
     4501 ccggcaatcg tcggcagcag tgttctgaca aacgcaataa agcgaccaat taacagcgcc
     4561 gataaaccgt gtttatgaaa aagatggtgt gcgcgttgat gataatgcgc gggtaaatga
     4621 gatagccagt tttgtacggt gcgggtattg cccagccatc gcccctgaat atagctgacc
     4681 cagcagccga ggctggcggc aacggtcagc agcagaatcg tttgcggata gcccatcgcg
     4741 cctttcgcaa tcaatacacc gaccaacacc agtaagctgt cgcccggtaa aaaggctgcc
     4801 ggaagcaagc cgttttcaag gaacaaaatg acaaacaaga caaaatacaa catgccaatc
     4861 atcgacggat tggccagggt ttcaaaatcc tgcgcccaca gggcttgcag caattgggtc
     4921 aaaagttcca ttcattattc ctggtgaatc ggttaacgcc acgctggttt taaatgatgc
     4981 actaccgcaa tgttattgct gtcttggtat gttctgcggg tttgatacat caacacagcc
     5041 tgaactattc cctgttaaat cgtgagataa gaaagcattc aaaagcataa attcaaattg
     5101 aatctaattg taacaaaggt caacgttgtt cgttatcaaa attacatgtc cgtggagtct
     5161 gattttgctg aatgcagggg agggaggcga gaggatttac acaggctgac actttatact
     5221 tttcgcttac cgacgagaca aacaggcgca ctgtagcgcc tgtcggacag gaatttcatt
     5281 tactgccgct ggtggctgta tcgagatgca ccaccgggtt ttcggcgaac agatagcggt
     5341 cggcattgaa ctcgaaatcg tcgctggttt cgttaaataa catgatcttg gtgttttcca
     5401 ggtgctgcca cattgccagc ttagcagcat gaggatcttt gcgaatcagc gccttgagga
     5461 tttgatcgtg gtcatcacac cagttatcga cggtacgggc atcaatgtgt tcgtgcagtt
     5521 ttttccagta cgggttatga ctacgctggg tccacatttt ttccacgata gccgccaggg
     5581 cggagttctg cgtcgccagg gctacctgaa tgtggaactg caaatcccac tcggaatcac
     5641 ggaagcattg ttcgccgcgc gcctgctcct ggatggccat cagtttcatg atgtcctgtt
     5701 tcgtcacctg agttgccgcg aactcggcaa tattactttc gatgagctgg cgagcctgga
     5761 gcaactcaaa cggaccgtaa ttggcgaatt ccatattatt gtcagtcgcc tgctgatggc
     5821 gcggctggtt ggaaaccaca tgaatacctg aacctttacg cacttcgaca tagccttcaa
     5881 cttccagcat gatgatggct tcacgaacca cggtgcggct gacgttcttt tcatcggcaa
     5941 taaagcgttc agcaggcagt ttatcaccca ccagatagac gccctgttcg atgcgctctt
     6001 tcagatcagc ggcaagttgt tgatacaaac ggcgtggttc agtgatttcc atatgcgctc
     6061 caggcatgat acggcagact atatttgtta taccactttt gcgagtttag ctctactcac
     6121 agtgatgaaa aagccgcctg agaaggcggc tttgaaggga aactaaaact tacacgaagc
     6181 cttaatgttg cggtgcggga tcgttatgtg attcctgcgc cacctcaatc gccggtttgt
     6241 tctgcaacac ggtccagata accagcgcac ctaacaggtc gaacactgcc agaactgcga
     6301 acagcgggct gaagccgatg gtgtcagcca gtgcaccgac taccagtgca aacagcgtac
     6361 ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg ttacgaccga
     6421 acacgtcaga cgagagcgta atcagtgcgc cagacagtgc ctggtgagca aaaccaccga
     6481 tacacagcag cataatcgcg acatacgggt tggtgaacag accgatcata cccgggccaa
     6541 tcatcagcac cgcacccagc gttacgacca tcttacggga aacgatcagg ttcacaccaa
     6601 accaacgctg gaacagtggt ggcaggtaac caccgaggat acaaccgagg tcagcaaaca
     6661 gcatcggcat ccaggcaaac atcgcgatct ctttcaggtt aaagccgtaa actttaaaca
     6721 tgaacagcgg gatccacgcg ttaaaagtac cccatgccgg ttctgccagg aagcgcggca
     6781 gcgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt ttcttcgctg
     6841 tgtcaacctg gtgctgggct tcctgaccat taataatgta gtcgcgttct tcatcggtca
     6901 gatgtttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc cagataaagc
     6961 tcaacgcacc ggagatgata aatgccatct gccagctgtg catcacgatt gcccatacca
     7021 ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac gttaaagtaa cctaccgcga
     7081 tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc ggaatcatcg
     7141 cggcttccgc ggcaccgacc gcaccacgag caacagccag gccgccccag ctacctgcca
     7201 gcgcggttgc accacagaac acggcccaca gcacagcaaa cattgcataa ccgattttcg
     7261 tacccagcac atccagtaca tagcccgcta ccggttgcat gaccgtataa gcagcagaat
     7321 aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc atcagagttg
     7381 gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgccg agcgtcacca
     7441 gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc atcgttttgt
     7501 tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta gttacgaccg
     7561 ggtaaccgaa aaaaaaccgg cacgccccga acgttgccat gaaagtttcg tgctaattcg
     7621 gcttccgtac cggttaaccc gattcatcta acaaatgaat gaatcggcaa tttgcattcc
     7681 gtcgacttat gatttgcgac ggcagaaaga taacttgtca tacaacttta aaaggtgaga
     7741 gccatcacaa atgtgcgaat atttgtaggg acattacctg acgacggcga ggccagtact
     7801 ggcgcggcct gcagcgagat ttaccacttt gagagtaatt tttttaacta cgtttattga
     7861 tctaactcac gaaaatatct tcggactctg gaaattggtg tgataacttt gtcagcatcg
     7921 caccataagc aagctagctc actcgttgag aggaagacga aaatgactcc gtttatgact
     7981 gaagatttcc tgttagatac cgaatttgcc cgccgtctgt atcacgacta cgcaaaagac
     8041 cagccgattt tcgattacca ttgccatttg ccgccgcagc agattgcgga agactatcgt
     8101 tttaaaaacc tgtatgacat ctggttgaag ggcgatcact acaaatggcg cgctatgcgt
     8161 accaacggtg tggccgagcg tctgtgcacc ggtgatgcgt ctgaccgtga aaaatttgac
     8221 gcctgggcgg cgactgttcc gcacactatt ggcaacccgt tataccactg gacgcacctc
     8281 gaactgcgtc gtccgtttgg tatcactggc aaattgcttt ctccgtcaac tgccgatgaa
     8341 atctggaacg aatgtaacga actgctggcg caggataact tctctgcgcg cggcatcatg
     8401 cagcagatga acgtgaaaat ggtcggcacc accgatgacc cgatcgattc tctggagcat
     8461 cacgcagaga tcgccaaaga cggctctttc accatcaaag tgctgccgag ctggcgtccg
     8521 gacaaagcct ttaacatcga acaggcgacc tttaacgact acatggcgaa gctgggtgaa
     8581 gtctctgata ccgacatccg ccgctttgct gatctgcaaa ctgccctgac caaacgtctg
     8641 gatcacttcg ccgctcacgg ctgtaaagtg tctgaccacg cgctggatgt ggtgatgttt
     8701 gctgaagcga acgaagcgga actggacagc attctggcgc gccgtctggc aggcgaaacc
     8761 ctgagcgagc acgaagtggc acagttcaaa actgcggtgc tggtgttcct tggtgccgaa
     8821 tacgcacgtc gcggctgggt acagcagtac cacatcggcg cgctgcgtaa taacaacctg
     8881 cgtcagttca aactgctggg gccggatgta ggctttgact ccatcaatga ccgtccgatg
     8941 gcagaagagc tgtctaagct gctgagcaag cagaacgaag aaaacctgct gccgaaaacc
     9001 attctctact gcctgaaccc gcgcgataac gaagtgctgg gcaccatgat cggtaacttc
     9061 cagggcgaag gtatgccggg caaaatgcag ttcggttccg gctggtggtt taacgatcag
     9121 aaagacggta tggaacgtca gatgacccaa ctggcgcagc tcggtctgtt gagccgcttt
     9181 gtcggtatgc tgactgacag ccgtagcttc ctgtcataca cccgtcacga atacttccgc
     9241 cgcattctgt gccagatgat cggtcgctgg gtggaagcgg gcgaagcacc ggcggacatc
     9301 aacctgctgg gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga ctacttcgcc
     9361 attgaactga actaaggtct ggggttgata tgcaatacat caagatccat gcgctggata
     9421 acgtcgcggt cgctttagca gatttggctg aaggcacaga agtcagtgtc gataaccaga
     9481 ctgttacgct gcgccaggat gttgctcgtg gacataaatt tgcgttaacg gatatcgcaa
     9541 aaggggccaa cgtcatcaaa tatggcctgc cgattggtta tgcattggcg gatattgcgg
     9601 cgggggaaca cgtccacgcc cacaatacgc gcacgaatct gagcgatctg gatcagtatc
     9661 gctatcaacc tgattttcag gatctgcctg cgcaagcggc agatcgtgaa gtgcagatct
     9721 atcgtcgcgc taacggcgat gtcggggtgc gtaatgagct gtggatcctg ccaaccgtgg
     9781 gctgtgtcaa cggcatcgcg cggcagatcc aaaatcgttt cctgaaagag accaacaacg
     9841 ccgaaggtac tgacggcgtg ttcctcttca gccacaccta cggctgctca cagctgggcg
     9901 acgatcacat caatacccgc accatgctgc aaaacatggt gcgccacccg aacgcaggcg
     9961 cagtgctggt gattggtctg ggctgtgaaa acaaccaggt tgccgcattc cgtgaaacgc
    10021 tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag gatgatgaga
    10081 tcgaagccgg gatcgcgcat ttgcatcagc tgtataacgt gatgcgcaac gataaacgcg
    10141 agccaggcaa actcagcgaa ctgaagtttg gtctggagtg cggtggttct gacggtcttt
    10201 ctggtattac tgctaacccg atgctggggc gtttctctga ctacgtgatt gctaacggtg
    10261 gtactaccgt actgaccgaa gtgccggaga tgtttggcgc agagcagttg ctgatggacc
    10321 attgccgcga cgaagcaacg tttgaaaaac tggtcaccat ggtcaacgac ttcaagcagt
    10381 actttattgc ccatgaccag ccgatctacg aaaacccatc accggggaac aaagcaggcg
    10441 gtatcaccac gctggaagat aaatcgcttg gctgtaccca gaaagcgggt tccagtgtcg
    10501 tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc ggggctgaac ttgttaagtg
    10561 cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg tgcgggctgc catatggtgc
    10621 tgttcagtac tggtcgtggc acgccgtatg gtggatttgt gccgacggtg aaaatcgcca
    10681 ccaacagtga actggcggcg aagaaaaaac actggatcga ctttgacgcg ggtcagctga
    10741 tccacggtaa agcgatgccg cagttgctgg aagaattcat cgataccatc gttgagtttg
    10801 ccaacggtaa gcaaacctgt aacgagcgta acgacttccg cgaactggcg atcttcaaaa
    10861 gcggcgtaac gctataaagt cgaaatgagt tgttaataaa aaacggcgtt tcataatgtg
    10921 aagcgccgtt tgcttttcca gacctgctaa ggatttatca tgaccgcgta ttggctggcc
    10981 cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa catttttcaa ccgtgacgaa
    11041 cgccgcttta aaaagcagct ttcggtctat agcgccgtta ttggcgtaca tttttttctt
    11101 ctgggcacct atcccgccgg tgccagtgcc atccttaatg ccattcgtac gctgattact
    11161 ttacgcacgc gcagcttatg ggtaatggcg atttttattg tgctgactgg cggaattggc
    11221 ctcgcgaagt tccatcatcc tgtcgaacta ctgccggtaa tcggcacgat tgtcagtacc
    11281 tgggcgctgt tccgctgtaa agggctgacc atgcgctgcg taatgtggtt ttcaacgtgt
    11341 tgctgggtga ttcacaactt ctgggcgggg tcgataggcg gcacgatgat tgaggggagt
    11401 tttctgctga tgaatggtct gaatatcatt cgtttctggc ggatgcagaa aaggggaatt
    11461 gatccgttta aagtagagaa aacccctccc gccatagacg aaaggggtta aacaattaat
    11521 tacgcagggc gctatttgcc agacgatcgt cttccgcctg gcaagctgcc gcagtgaaca
    11581 gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc ctgcaatacg ccgatgataa
    11641 agccgacggc aaccacctgc atggcgatat cgttcgaaat accgaacata ttacaggcca
    11701 gtgggatcag cagcagagac ccccccgcca cgccggatgc gccacaggca cacagagaag
    11761 ccactacgct caacagcagc gctgtgggca gatcgaccgg aatacccagc gtattaaccg
    11821 cagccagcgt caacacggta atggtgattg ctgcgcccgc catattgatg gtggctccca
    11881 gcggaataga aacagaatag gtatcgcgat ccagattcag cttttcacac agcgccatat
    11941 tcaccggaat gttcgctgca gagctgcggg tgaagaaggc atacacaccg ctttcacgca
    12001 ggcacagcag caccagcggg aacgggttac gacgaatttt ccaccacacc agcaatgggt
    12061 taaccaccag cgccaccagc agcatacagc caaccagcac gaccagcagt tgcgcgtagc
    12121 cccacagcgt ggagaaaccg gtggttgcca gggtagaaga aaccagacca aaaataccga
    12181 tcggtgcgaa gcgaatcacc agtttcacca taaaggtgac ggcattcgac atatcgttga
    12241 tcaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa accgaggcca attgcccaca
    12301 ccaggatccc gatatagtta ccttttagca gcgcgtcaat ggggttggaa accatgctca
    12361 ttaccagccc gcgcattact tctacaatgc ctgacggcgg cgaaatatca cccgcgctac
    12421 tggacaagtg cagggtagaa gggaaggcaa aactgaagat tactgcggcc agagcagcag
    12481 agaaggtgcc cagcagatag aggaacaaaa tagggcggat attggttttc tgcccgtgct
    12541 ggtggttagc aatagatgcc attaccagca tcaacaccag gatgggggca acggctttca
    12601 gtgcgccgac gaacaaagta cctaacagac caacagcttc cgccgcgggt tttgagatcc
    12661 atgccagaag aatccccaga acaaggccga ccaggatttg ttttaccagg ctgccatgag
    12721 ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc tttcattgtg
    12781 ttgtcgcaca tccttggtgc gttctgtcga cgcatctttc aggatgtaac aaagtgtttg
    12841 caaggtcgag tataaggaaa gttacagctt cgggaagcga aaaatgctgg attttaaaga
    12901 gaggtcatat tttgcaactt aatggttaca tttatttgac ataaacagag ggaaggactg
    12961 gctactaagg ctgattagat aacatatggg caaaactatt tggaatatga gaacggttgc
    13021 gctcctttaa tgtgctatgc catataaagc cactggcgca gggagcgcgc agggggcggc
    13081 caatcgccgc cgccccctgc acccccaggc tctggcgaac aaaatcgccg ctgcgcggtt
    13141 ccctcagctt atcccttgcg gctaccgggt cgggcgcaag ggaacgtccc tgtaagatgc
    13201 gccctcgccg cgcatccatg cgcgtcgccc cggcctccag ggaacgcttc ggcgattttg
    13261 acgccaccaa cacccgtgcg gtctttttgt aaagagctat aagccgatta cataattaat
    13321 agctcacctt tgtctggaat gagttatctc tagtaatggc ttaatataca gcgaatcatt
    13381 ctccccgctg cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca
    13441 gaatgccaaa caccacgccc agcgagacgg cgatcggaat atggtagaag tcgacaatca
    13501 gcatcttgat accgataaac accagaatca ccgccagacc atatttgagc atcgagaaac
    13561 gctctgccac acctgccagc aggaaataca tcgcacgcag gccgaggatc gcaaacaggt
    13621 ttgaggtcag cacaataaac ggatcggtgg ttacggcgaa gatagccgga atactatcca
    13681 cggcgaaaat cacgtcgctc aactccacca gaatcagcac cagcatcagc ggcgtggcat
    13741 acaacaaacc atttttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca
    13801 aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg
    13861 cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt
    13921 cgaactgtga aatcagccag ctgccagtga agatcatgat ggtacgcaga acaatcgccc
    13981 cgagcacgcc atacaccagc acgcggcgtt gtaatgccgc cggaacagag aaatagctga
    14041 acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc agataacccg
    14101 tgagaaaggc cagtgcctgt ggatcggcaa cggcgcgacc ttcggtttgc accagatacc
    14161 accagaaagc ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag
    14221 cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca
    14281 gcataatggt gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca
    14341 tagaagttcc ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt
    14401 atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg
    14461 tattcctttc gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg
    14521 accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca ttcagtcagt
    14581 caatggtctg ttacatacct tttacactgt tttactttgg caattatagt tttacgctat
    14641 ctgccggaaa aatcacccca gtctggcggc ggatctcggt cagcagttta gcggtgatgc
    14701 gactgaccgc cagccctgga tgatccacca gatgctcatc caccagttca gcaaacagct
    14761 ctgcttcgta gagcatggta ttaatatgct gcggttgggt cagatcctgc atctggctgc
    14821 cacgcggcac aaaacacact ttctggcatt cagacagttt ttcaatcacc agcgatcccg
    14881 cttcgccctg aatctcgctt gccaggacag aatcgctcac ttttgagtgc tgcaacgtaa
    14941 cgctgaaatc accgtaatcc atcaccacca cgccgtgggc atccacaccg cttgccagca
    15001 aactggcggt tgcctgcacg tttttcggtt caccgaataa tgccaccgcc gacgccagac
    15061 agtaaaagcc gatatccata attgaaccgt tagagaatgc tggattaaag gtgttgggat
    15121 tctcaccatc caggtaacgt tgataccgcg aggaatactg gcaatagttg aaaaagactt
    15181 tacgcagttt gccgactttc ggcagcgcct ggcgcaacag atgaaagttc ggcaggtagg
    15241 ccgttttaaa tgcctcaaac agcaccacct gattttcccg cgcacaggca atggcggcat
    15301 ccacttccgc cagattcgat gccagcggct tctcgcaaat cacatgaatt ttgtgactaa
    15361 ggaaaagttg tgtctgggaa aagtgcaggg cattcgggct ggcaatatac accgcgtcaa
    15421 tggcatcgct ttccgccatc gcttccagcg acgtaaacag atgctcgaca gaaaaatcat
    15481 tggcaaagtg ctgggcctgt tcaaggctgc gggaatatac ggcggttaac ttgtatttac
    15541 cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga
    15601 aacgtatcat aacgcgtgga tactccataa agggattctg ccgtcaatgt agcacgcctt
    15661 tttcatcttc gacgtgcgca gatacgcatt atttctgcgc cggacagcca tgctcactaa
    15721 acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg atttttgcgt
    15781 caatcaccgc gccgacgcga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca
    15841 tattgaggtc tggcgcgtta aaaccgatag cgtcgatgcc gtactgtttt gccagccaga
    15901 tggcgcgttg gttatgaaat tcttgcgaaa taatggtgat atggttttcc ccaaacacct
    15961 ttttggcacg caccaccgaa tcaagcgttg agaatccggc atagtcgcag aagatcactt
    16021 tcgccggtac gcctttagcg atcaaagcct gctgcatacc tgaggcttca tcgtaatttt
    16081 tacgcccgtt atcgccactt accagtagcc atttcacttt tccggcgtgg tacaacgctg
    16141 ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctcgccccc agcaataaac
    16201 ccacgttacg cgccggaact gcgctgacat cgctccaggt cagctgcttg ctggtgttga
    16261 ccataacgcg atcggcaata aaaatggtcg ctcctgctat aagcaacaat aaacagccaa
    16321 ttttaagagt ccggcgagaa aaacaaatac ggagaagaag gcgggcctgc atggtcgtta
    16381 tccttaacga aaggggggag gtcgatttta ggcgctatag gaagaaaaga taacgcctga
    16441 catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg
    16501 aactcaagct ggtgatactg cggctccata tggcagcaca attgatagaa cgctttgtta
    16561 tgctctttct ccttcaggtg cgccaactcg tgcaccacga tcatgcgcaa aaacggttcc
    16621 ggcgcgttgc gaaacacggt agcgacgcgg atctccgcct ttgctttcag cttgccgccc
    16681 tgcacacgcg aaacggcagt atgtaatccg agcgcgtttt tcagcacgtg gatcttattg
    16741 tcatacatca ctttagtgat cggcggggca ttgcgcagaa actgattttt aagatcctga
    16801 gtatattgcc agagggcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc
    16861 agcacatctc ccagacgctg ttcgttaatc agcgttcgca cctgggaaag tagctgctcg
    16921 ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt
    16981 atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga
    17041 gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg
    17101 ttaacccact acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca
    17161 cagaaatccg cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agttgtgcgc
    17221 tggcggaaca taagccatac agcattggcg actcatacat cagcgaactg gcgacgcgcg
    17281 agaatttacg cctcaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg
    17341 actacccgca acagccgggc gtggtactga tcaaagtacc gaaaacactg gcgttgctgg
    17401 aacaacaact gcgtgcgctg cgcaaagtgg tcacgccgga tacacgtatt attgccggtg
    17461 ccaaagcccg tgacatccac acttccacgc tggaactgtt cgaaaaagtg ctcggtccga
    17521 ccaccaccac gctggcatgg aagaaagcgc gcctgattaa ctgcaccttc aacgaaccgc
    17581 cgttggccga tgcgccgcag accgttagct ggaagctgga aggcactgac tggactatcc
    17641 acaaccatgc gaatgttttc tcccgcactg ggctggatat tggcgcgcgc ttctttatgc
    17701 aacatctgcc agagaatctc gaaggtgaga ttgtcgatct cggttgcggt aatggcgtta
    17761 ttggcctgac attgctcgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcac
    17821 cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgcccgag gcgttggatc
    17881 gctgcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgt tttaatgctg
    17941 tgctctgcaa cccgccgttt catcagcaac atgcgctgac cgataacgtc gcctgggaga
    18001 tgttccacca tgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc
    18061 gtcacctgga ttacttccat aaactgaaga aaattttcgg caactgcacc accatcgcca
    18121 cgaataataa atttgtggtg ctgaaagcag tgaagctggg gcgtcgtcga taagattggg
    18181 agttacctgc cggatgcggc gtaaacgcct tatccggcct acgttcagca cattaccccc
    18241 aggtcggata agacgcggca agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc
    18301 gcttccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt
    18361 ctacgtttat gctacatacg tgaagttatg acgcataaag aatacgattt ctttaccgcc
    18421 gttcatcgta ctaaggggaa aaaatgattg cgattgccga catcttgcaa gcaggagaaa
    18481 agctaactgc tgtggcacct tttctggcgg gtattcagaa cgaggaacaa tacgcccagg
    18541 cgctggaact ggtcgatcat ctgctgctca acgatcctga aaatcccttg ctggatctgg
    18601 tgtgtgccaa aataaccgcg tgggaagaat cagcgcccga atttgctgaa tttaatgcca
    18661 tggctcaagc catgcctggc ggtatagccg tgattcgtac ccttatggat caatatggtt
    18721 taaccctttc cgatcttccg gaaattggca gtaaatccat ggtgtcacgc gttttgagcg
    18781 ggaagaggaa attaacgctg gaacacgcta aaaaactggc aacgcgattc ggcatttctc
    18841 ccgccttgtt tattgattaa cgcgacgtgc cggatgcgtc gcatccggcg cttcaaacaa
    18901 cgatttaaat ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt gcgtcaagct
    18961 ccatagctac atcgcagccg ccaattaaat gcacagtttt cccgctatca atcagctgtt
    19021 gcgccagcgc gcggtttggc tcttgccctg cgcagatcac cacattgtcc actgctaata
    19081 cctgcgtttc gccgttgatc accacatgca gcccgtcatc gtcaatcttc tgataactta
    19141 cgcctgggat cattttcaca ccccgcgaga gcagggtggt gcgatggatc cagccggtgg
    19201 ttttgcctaa cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca atctgccgtg
    19261 ggctacgcgg gatctgcatt ccctgcgggc ttaagccacc agcctgttgc aggctgctgt
    19321 cgatccccca ctcattacag aactcggcaa tgttctggct ggtggactca cccggctgac
    19381 ttaaaaacat cgccgtatca aaaccaatcc cgccacaacc gatgattgct acctttttgc
    19441 ccacaggcgc tttgtcgcgc agtacgtcga gataactcaa caccttcgga tgatcgattc
    19501 cgtcgatagg tggaatgcgc ggtacgatcc cgctagcgag gatcgtttca tcgaacgact
    19561 gtaactgatc cgccgtcacg gtgtgattga gtttcagcgt cacgcctgtt acttcgatca
    19621 tccggcggta atagcgcagc gtttcgtaaa actcctcttt gccagggatc tgtttggcga
    19681 tattaaactg accgccgatc tcactgtgag catcaaacaa cgttacctga tgaccacgtg
    19741 ccgccgcgtt aatggcaaac gccagcccag ccggaccagc gccgaccacc gccagatttt
    19801 ttttctgcac ggcgggaagg atcggcattt tggtttcgtg gcaggcgcga ggattcacca
    19861 ggcacgaggt gactttgcca acgaagatct gatcgaggca ggcttgattg cagccaatac
    19921 aggtgttgat ctcatcggct cgccccgatt gcgcttttga cagcagttcc gcatcagcaa
    19981 gaaacggtcg cgccatcgat accatatcgg catcaccgcg cgagagaatg tcgtcggcaa
    20041 cctgcggatc gttaatccgg ttggtggtca ccagcggcag cgagacgtgg cttttcagtt
    20101 tgcgcgtgac ccagctaaag gcaccgcgcg gcacgggcgt ggcaatggtc ggaatacgtg
    20161 cttcatgcca gccaatgccg gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg
    20221 ccagccctac cgtttctgca aaagtaccgc cgccttctac caggtcgagc atcgacagac
    20281 ggtagataat aatgaagtca ttgccgacgc gttcgcgcac tgctcgcacc acttcaactg
    20341 caaagcgcat ccggttgcgg taatcgccgc cccactggtc gctacgctgg ttggtgcgca
    20401 gggtcagaaa ctcgttgatc aaataccctt cggaacccat cacttctacg ccgtcgtatc
    20461 cggcctcccg cgccagttgc gcgcagcggg cgaaattgtc gatcagttgc aggatctctt
    20521 catggcttaa ctcatggggc acgaaacggt tgatgggggc ctgcaatgcg gagggagcga
    20581 ccagatgcgg ttggtagctg tagcgcccgg tatgcaaaat ttgtaaggca attttgccgc
    20641 cttcctgatg tacggcttcg gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga
    20701 gcattgcgcc gccttccatg ccaacgcctg ttaaatctgg tgcaatgccg ccgctgacaa
    20761 tcaacgccac gccatgacgg gcgcgttccg cgtaaaacgc tgccagccgt tcagcgccgt
    20821 ccgggtattc ctccagtccg gtgtgcattg aacccatcag tactcggttt tttaacgtgg
    20881 tgaagcctaa atccagcggg gcgaacagcg acgggtagct cataaaattg tccagtatgt
    20941 aaaataattg ttatgtggtc ggatgagttc taatttagcc gtcgctggag aaaagggaaa
    21001 aggggagtgc ggggtttgtg atgggattca aaaagaatac gcccgttcgt catgccggac
    21061 ggcggtaata cagtcgtccg gcatcgggaa aaattactgc ttacggaaaa aatcgttata
    21121 caacatatac aaatgcgcgg cactccagga gaaatttggt gcgccttgct gtgcgccagt
    21181 cagcgggttg taattctcct gaattgggcc atcggcggtt aatcctttgg cgtgccggaa
    21241 gaacgtatcc gccagcttca gggcatcatc gcgataaccg taacgctcca tccctttcag
    21301 accaaaccag aactgatcca cccacacgcg cccgcgccag taaatatctg cgccaaaggc
    21361 cggattggtt aacgccgccg ttcccagcgg tacaaaggtg ttgaactctt taggatcgag
    21421 catcaccttc accaccgcgt cggcgttggc ctgcgttgcc gcaccgttaa acagcggcga
    21481 ccagccttcc ggccctttgc cgcgctcgac aatcggtttg cccgcgcagc cgttcgccag
    21541 tggtttatct tcaatgcgca cgtcatagta gaactgggta gtcgggtcga acatacaggt
    21601 gttgatgtag tccgcgagct gttgtgctaa ctgacgatag cgtttggcct cttccggctt
    21661 accgagaatc gtcgccatct ccgccagata atggttatcg ctgtacatat agctggcctg
    21721 atccaccgac tcctgcaata gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc
    21781 ggcgaatttc accgtccagt cgctacgttt gccgccgtta gcgacatatt tatccagctg
    21841 ttctttgtcg ataaacccaa agacggcggc atcatcacgc cccgattccc acgacgccgc
    21901 aacctgtgcc gggatttcca gactgtcgta ctggcctttc tctaccacgc gggcgtagtt
    21961 gttcagccca gacagcgtct ctgctttgtt gcctttcttc accgtaaaca gcatctcgcc
    22021 gctctcagtg ttgtgggctt tgtcgcgggt cgcgccatat tccggcacgc cgttgccgtt
    22081 gtgatcgcgg ttacgtagcc accagtcgtg ataggccacc agtttcgggt acatctctgc
    22141 cagccaggtt ttatcctggg tgacgttata cacttccatt actgaccagg cggcaaggct
    22201 gggtttggta ttacgtttgt tccagttgcc gccatcgccg ccacgctcgg ggctaaggtt
    22261 ccatgctatc aggtcgggga caaagcccac atcctgcgga cgcacgcggt cgcctggctg
    22321 gatctgccag gagaagaccg cgcggatatt ctctttggcg atatccggat tgaaatgcgc
    22381 catcgcaaat gcctgcttcc aggtatccca cggccaggtc tgattgccgg agaaccagcg
    22441 cccggtcacc gacggcgtga cggtgttata tttcaccgcc cccccaggcg agcgccagtt
    22501 accgttgagc gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg
    22561 attggttaac cctttcttca gatactcttc ccagcgttgc tgcgaggcgg tgagataaaa
    22621 cgccggacgc gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt
    22681 cagcaggtgg gagtaggtgg tatagagcgt ggccgaaccg ttgatatgcg ccttactggt
    22741 aaagcgatta ccgttgattt cagtctgcac tggcagggat ttatgcacct gatattctga
    22801 ttcaccggag gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc
    22861 atcacgggtg gcgctgattt tgcgctgata gtcagggtat tcgccagcaa tggttttatc
    22921 ggaaagcggt ttcccttctt tcgcttccag tttttccagc agctcgccgt cccacaccag
    22981 atccagcggt ttattgctga tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa
    23041 gcgcagagtc atttcgacct gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg
    23101 aatactgtat gcctccagcg tgaagtcgac tttcttgccg tcttgccaga cggtcagacg
    23161 atcgaaattg ctggccataa agttgatgta ttcttccgtc agcagcgcaa cgcctggaaa
    23221 accgcccatg gtgttcgggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa
    23281 aaacggatta aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcgcc
    23341 agtacggtta atcacgtttt tataattgtc ggcgttagcg gcatgggcgg aaaaacttat
    23401 cagcagagcg caggttactg gcgttaaaat agttttgatt ttcatgaaat tacagtcctt
    23461 attaccagta gaaatattgc atcaggaaga gttggtcgct gggatcgtga gaggcatttt
    23521 tcatgtagaa gcggctatca aaagccgttg cgaaacgtcc gccgccgtat tcgtaccaga
    23581 taccaaactg catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt
    23641 aggtgtaagc ggtggagaga taaatgcctt ttaacgcttc atacatggcg ctcaccatca
    23701 tcccgctttc acttcccgat acgtccagcc cgtcggcgcg tccggtatgg tgccaggcgc
    23761 gagcggcaaa gttaggggta agcaggcccg tcagatagag ttggcctgtg ccgtcggtga
    23821 tttccagacc gttcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca
    23881 tcattgccgg ataggtgtat ttgtcgtcgt agcgatcgta cttgccaaaa tgtagccagg
    23941 ctttgctttc gtcatgatgt ccggcaggtg tggcggtaac gctataacga aagttgccgc
    24001 ttaagttctg caccttcagc gcggtcataa tgtcgcgggt attgggaatg acgtagccca
    24061 gatcgggcgt gaaatcgccc caccattgca ggtcatccag cgaggaatcc tgacgcagac
    24121 tgagcatgat ttcggtgcca tcattggtac gataaccgcc atagaaacgg ttaatcccac
    24181 cttcgaaacc gccccagcta tggtcaggca cccaggcatt accgtcgtgg tcggcctgca
    24241 cggtccagcc ttcgccgtaa atcaggccga accaggatcc gtacttcatc tccaggccac
    24301 cttcaatata ggtgccgtcg ctgtatttat gtttattctc accctctaaa tccatatacc
    24361 caccaacccc taattcgccg taaaaacgaa atgaaggaga agatgtcgag gtcattgcgg
    24421 gggtttttgt gtcgtcatgt tctgccggac gtgcttcatc agcggtcaca gaaaaggcgt
    24481 agcaaaacgg cagcgcaagt aatgctctgc atggtgcagt gattaatttc attgtgttaa
    24541 tccatgagct aaagaaaaag tacagccttt tatcgttgta attataaaaa tataaaggca
    24601 gaaactattt tccctctccc catccaggaa gagggaaaat gcctgctcat tttttattat
    24661 tctgcttaa
//
LOCUS       DAZKJR010000074        24187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKJR010000074 DAZKJR010000000
VERSION     DAZKJR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.074"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..261)
                     /locus_tag="ACHOWS_002966"
     CDS             complement(<1..261)
                     /locus_tag="ACHOWS_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0048511.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSL"
     gene            complement(258..1928)
                     /gene="menD"
                     /locus_tag="ACHOWS_002967"
     CDS             complement(258..1928)
                     /gene="menD"
                     /locus_tag="ACHOWS_002967"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFQ0048512.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2017..3312)
                     /gene="menF"
                     /locus_tag="ACHOWS_002968"
     CDS             complement(2017..3312)
                     /gene="menF"
                     /locus_tag="ACHOWS_002968"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFQ0048513.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGEIAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3391..3696)
                     /gene="elaB"
                     /locus_tag="ACHOWS_002969"
     CDS             complement(3391..3696)
                     /gene="elaB"
                     /locus_tag="ACHOWS_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFQ0048514.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3751..4212)
                     /gene="elaA"
                     /locus_tag="ACHOWS_002970"
     CDS             complement(3751..4212)
                     /gene="elaA"
                     /locus_tag="ACHOWS_002970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0048515.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            4277..5194
                     /gene="rbn"
                     /locus_tag="ACHOWS_002971"
     CDS             4277..5194
                     /gene="rbn"
                     /locus_tag="ACHOWS_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFQ0048516.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCNA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPVTELANDFTVFNV"
     gene            5382..5495
                     /locus_tag="ACHOWS_002972"
                     /pseudo
     CDS             5382..5495
                     /locus_tag="ACHOWS_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            5550..6521
                     /gene="yfbL"
                     /locus_tag="ACHOWS_002973"
     CDS             5550..6521
                     /gene="yfbL"
                     /locus_tag="ACHOWS_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFQ0048517.1"
                     /translation="MKKMTSLCFIPLLLSLTFIIFYQPWVRALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQLQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            6624..7127
                     /gene="yfbM"
                     /locus_tag="ACHOWS_002974"
     CDS             6624..7127
                     /gene="yfbM"
                     /locus_tag="ACHOWS_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFQ0048518.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(7194..8651)
                     /gene="nuoN"
                     /locus_tag="ACHOWS_002975"
     CDS             complement(7194..8651)
                     /gene="nuoN"
                     /locus_tag="ACHOWS_002975"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFQ0048519.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(8658..10187)
                     /gene="nuoM"
                     /locus_tag="ACHOWS_002976"
     CDS             complement(8658..10187)
                     /gene="nuoM"
                     /locus_tag="ACHOWS_002976"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFQ0048520.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(10351..12192)
                     /gene="nuoL"
                     /locus_tag="ACHOWS_002977"
     CDS             complement(10351..12192)
                     /gene="nuoL"
                     /locus_tag="ACHOWS_002977"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFQ0048521.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(12189..12491)
                     /gene="nuoK"
                     /locus_tag="ACHOWS_002978"
     CDS             complement(12189..12491)
                     /gene="nuoK"
                     /locus_tag="ACHOWS_002978"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFQ0048522.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(12488..13042)
                     /gene="nuoJ"
                     /locus_tag="ACHOWS_002979"
     CDS             complement(12488..13042)
                     /gene="nuoJ"
                     /locus_tag="ACHOWS_002979"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFQ0048523.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(13054..13596)
                     /gene="nuoI"
                     /locus_tag="ACHOWS_002980"
     CDS             complement(13054..13596)
                     /gene="nuoI"
                     /locus_tag="ACHOWS_002980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFQ0048524.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(13611..14588)
                     /gene="nuoH"
                     /locus_tag="ACHOWS_002981"
     CDS             complement(13611..14588)
                     /gene="nuoH"
                     /locus_tag="ACHOWS_002981"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFQ0048525.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(14585..17311)
                     /gene="nuoG"
                     /locus_tag="ACHOWS_002982"
     CDS             complement(14585..17311)
                     /gene="nuoG"
                     /locus_tag="ACHOWS_002982"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFQ0048526.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(17364..18701)
                     /gene="nuoF"
                     /locus_tag="ACHOWS_002983"
     CDS             complement(17364..18701)
                     /gene="nuoF"
                     /locus_tag="ACHOWS_002983"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFQ0048527.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEVGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(18698..19198)
                     /gene="nuoE"
                     /locus_tag="ACHOWS_002984"
     CDS             complement(18698..19198)
                     /gene="nuoE"
                     /locus_tag="ACHOWS_002984"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFQ0048528.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(19201..21003)
                     /gene="nuoC"
                     /locus_tag="ACHOWS_002985"
     CDS             complement(19201..21003)
                     /gene="nuoC"
                     /locus_tag="ACHOWS_002985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFQ0048529.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(21097..21759)
                     /gene="nuoB"
                     /locus_tag="ACHOWS_002986"
     CDS             complement(21097..21759)
                     /gene="nuoB"
                     /locus_tag="ACHOWS_002986"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFQ0048530.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(21775..22218)
                     /gene="nuoA"
                     /locus_tag="ACHOWS_002987"
     CDS             complement(21775..22218)
                     /gene="nuoA"
                     /locus_tag="ACHOWS_002987"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFQ0048531.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(22848..23786)
                     /gene="lrhA"
                     /locus_tag="ACHOWS_002988"
     CDS             complement(22848..23786)
                     /gene="lrhA"
                     /locus_tag="ACHOWS_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFQ0048532.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         5971 a         6687 c         6185 g         5344 t
ORIGIN      
        1 aagcgagtac cccaccagcc agaagttaag gatgttgtaa ctaactaagg ttttacgcag
       61 taagtcggtg acatcatcaa atccatcgac gctaatcgcc gccgaaccac cgtgacctgg
      121 gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg cccacttctt gccattcatg
      181 gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt aaacctggtt taccgtgttt
      241 tgcctgcgcg tgcaggatca taaatggctt acctgcgcca gcagttgctg gagggtttgc
      301 gcaccatcgg tgtcgttaac caccatttca attaccgtgg tggtcggcgt gcgccaggcg
      361 tcggcaagtg ccgtttcaag ttcctgccag ttttgcggac gatgatattt cagctcgaac
      421 atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca gatagaaacg ctcacgctcg
      481 tttttcggcg ttggcaacag cgagaaaatt tgcccgccgt tgttgttcac cacaattaat
      541 accagcggcg cggaaacctg acgtaataac gccagcgcgt tgagatcgta aagtgctgag
      601 agatcgccca caatcgccag cgtcggtttg ccactggccc gctgaacgcc agcggcggtc
      661 gagagcagcc cgtcgatacc gctggcacca cggttgctgt acaccgggta acctgccgga
      721 agttgcgaaa gcgcatcaat cagacgtacc accaggctgt taccaacaaa caattgaccc
      781 tgttcaggca gatagtcgct gatgcgatgc gccagttgcg cttcgccaaa cgcatcgcga
      841 cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg gaatttcaac gcaccagggc
      901 tggcgttttt ctgccggatg ctgctccagc cagtcggcaa tattggcaat taagcgacgt
      961 ccacgatggt gtgccggatc aagtcgccct tcgatgtcat caacaatcca gtactcttcc
     1021 ggttcacagc ttgcctgcca ttgcaggagc cgtttgcccg tcaggctgct tcccagttgc
     1081 accacaattt gtgcctgttg cagttcgctg gtcgctttgg cattgcctaa ccagagatcg
     1141 gcacacggca gcggctgtcc ggtttgcgac agcacgtcgc caatcagcgg ccagccaaga
     1201 gtttgcgccc acagtgcaac ttttttgccc tcttccgcac tcatgcgccc ggcaaccacc
     1261 acgccgcgct tttgccgcca gaagaaccag tcgcgctgtt tttcactttc cagacgaggc
     1321 gcttcacgca gccacggttt gtcgtcctgc caccagtcgc ccagccgctg ttgccagcta
     1381 agcccggtgt cgtccatttc gccatacagc ggttcagcaa acgggcagtt gatatggact
     1441 ccgcccgcat gaagcgtacc gagagcatgg tcgatggtag aaaccagcca acgtgcagga
     1501 atatcctgag tcgggcgcgg cagtgaaata ctgtgcgtgg ggtgagaggc gaacattccc
     1561 ggctgacgaa tcgcctgatt cgcgccgcag tcaattagct ccggcgggcg atcggcggtt
     1621 aacaggatca gtttttctcc ggttaaccca gcttcaatca gtgccggata gagatttgct
     1681 accgccgtgc cggaggtcac aatcaccgcc accggctgct tgctgacttt cgccagcccc
     1741 agcgccaaat gccccagtcc acgctcatcg aaatgggtgt ggtgaatgaa tgcggaattc
     1801 tccgccgccg ctaacgttaa cggtgtagaa cgcgagccag gggcgataca gatgtgtctg
     1861 acgccgtgac gcgttaatgc ttccagaatg accgccgccc agcgtcggtt aaatgcgctt
     1921 actgacatga gtttgtccgg tatcaatatt gcggctaagt ataaggagct acaaaaatag
     1981 attattgata tgaatcggta atgatgcgac tcattactat tccatttgta ataaagtacg
     2041 cagccctgcc gctttgttat cgatttcctg ccactcttgc tcggggtcag aaccacggac
     2101 aatgcccgcg ccagcatata gccgcacgac attgccactg atttttgctg agcgcaggga
     2161 aacgcagaat tcgctttgtt gtaaagagag atagcccgca gaaccggcgt accattctcg
     2221 ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga tcgcgcggta agccagcaac
     2281 tgctgccgtt ggctgcaact gatgtaaaca gatcacatca tccgctttgt tgagtgcagt
     2341 ccagatacag cggcgaagat gctgcacttt acgcagacgc agtacctgcg gcggtaaaac
     2401 atccaacgtc tgggtatcgg cctgcaatcg ttggcagata tcctccacca ccaacatgtt
     2461 ctcgcgctgg tttttatcat ccgccatcag ccactcacct aactgctgcg cctgcttatc
     2521 atcaggatga tttgctaccg ttcccgccag cgcttcagta cgcagcgctt tgtcacgccg
     2581 ccgccataac cgttccggtg aagagccaag aaaagcaatt tcgccatcaa aggccatgta
     2641 aaaatggtag caattcagat tcagtcgacg actggcagcc atcatcgccg ccgcattgac
     2701 cggacttgcg aaatgcaggt cagttgcccg agcaagcacc actttgtcga gctcaccttc
     2761 ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag cccgttttgt ccggccagtg
     2821 ttgttctcgg gtggtggtta aatgtaaccc aggcaagggt ttaatactca ccagtgtggc
     2881 gataaattct tttgccttaa tcgcatcatg ctgaagggag ctttcgctga atagcgtcag
     2941 ccgcagcgtg gctttaccgc cacagcgtcg ccattcgagg cgtggtaaaa gtaaattgcc
     3001 ctgcgacggg tcaaacgcat ttagccccca gatgcgtaag tcggcgtgtt ccgggtgctg
     3061 acgaagaaac gtttgtgcct ggtccaacga cgtaaaacgg gtaatcgcgc ccaggacggc
     3121 agcttcttca tcaccattac gttgttgcca gtagaattgc gggtacgttt gctgactggc
     3181 cagccagctc aaggcatcaa aagcgtcttt gagagggaaa ggaatatcaa taacccgaat
     3241 gccgggtgtc gccggaatct cttgcgacaa atggcgcagt agattttcca gcgccgtagt
     3301 aagtgattgc acgcggacct ctccctgtta aaagcctcac attatacggg gtacgacaaa
     3361 aaaaatgcag taccccggtg tagggaggtt ttaacggcgt gccagcaaca gtcctagtac
     3421 cagcccaacg gccgcgccca caccaattcc ttgccagggt ttttcgtgga cgtagtcatc
     3481 agcacgataa acagcctgct tcgcccgata gtaataactg tctgaagcct ggctaacccg
     3541 ttttttcaca tcatccagcg ctttttctgc acgcgctttc agctcaacat atttctgatc
     3601 ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt tcactaagca gcgtcaggtc
     3661 atcatcaata cgtgtatcac caaactgatt agacattctc gttctccatt tgtgtaaaac
     3721 ctgtcactaa ctatagacaa gggttattga ttacgcctga attacctcgc gtgccatccc
     3781 aatgtgtggg ataccatctt cttcataaat atcggtcaca gggatgaaac caaattgccc
     3841 ataaaagttt tgtaaatgcg cctgcgcccc cagataaatc ggcttctccg gccagcggcg
     3901 agtacacgat tccagcgcct tactcatcaa ttgctgaccg attttttcac cgcgtaaggc
     3961 ttcgctgaca atcacccgac caatcacaac gggttgcagt tcgtcgtcac ttttcagaat
     4021 cctcgcatac gccaccagtg tcccgttatg ccaaccgaga atgtggcgat tttctccctc
     4081 cagatcgtca ccatcgatat cctggtaagg acagttttgt tccactacaa ataccgcaca
     4141 acgcagttgt aataaggcat aaagctgaga aacggataaa tcagaatgat gcagatcttg
     4201 ccagtcgatc atggaaggct cctttgtgct gtttgcttta tactagccgg aatattttca
     4261 gcagcgtgat gaactcatgg aattaatttt tttaggtact tcagccggtg tgccaacccg
     4321 tacgcgcaat gtcacggcaa tattacttaa cctgcaacat ccgacccaga gcggtctttg
     4381 gttgtttgac tgcggtgaag gcacccagca tcagctactg cataccgcct ttaaccctgg
     4441 aaaactggac aagattttta tcagtcacct tcatggcgat catctctttg gtttacccgg
     4501 cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc ttaacgattt atggtccgca
     4561 cggtatccgt gaatttgtgg agaccgcgct gcggattagc ggctcatgga ccgattatcc
     4621 gctggaaatt gtcgaaattg gcgctggcga aattttcgat gatggcctgc gcaaagtgac
     4681 cgcttatccg ctggaacacc cactggaatg ctatggctat cgtattgaag aacatgataa
     4741 accgggtgca ttaaatgctc aggcattaaa agctgctggc gtgccgcctg gcccactgtt
     4801 tcaggcatta aaagcgggca aaacaatcac gctggaagac ggaaggcaga ttaacggcgc
     4861 agattactta gccgctccag tgccaggtaa agcgctcgct attttcggcg ataccggccc
     4921 ctgcaatgcc gcacttgacc tggctaaagg tgtcgatgtc atggtgcacg aagcgacgct
     4981 ggatataacc atggaagcca aagccaatag tcgcggtcat agctctacac gccaggctgc
     5041 gacactggcc cgtgaggctg gagtcggtaa gctaatcatt acccacgtca gctcgcgcta
     5101 tgatgacaaa gggtgtcagc acctgttacg tgaatgcagg tcaattttcc cggtgactga
     5161 actagcgaat gatttcaccg tgtttaacgt ttaactctgc ttattattaa aaacagggcg
     5221 aaacttgccc tgttatcgca acccgcgcta catatatcca cccacacggg tatattgagc
     5281 gatttttgat atttattcat ctcctcacgc actatattca gagcaataag ttgcctcttt
     5341 ttatcttcat tcagcacaac ctttgcaact aaaaggagcc tatgatggtt acagttgtca
     5401 gcaattattg tcaattatct caaacccaac tctgtcagac atttgcagaa aaatttactg
     5461 tgaccgagga attactgcag tctttaaaaa aacacttata actcattaca aataatgatg
     5521 aaataacgct ttttattgat aatttacata tgaaaaaaat gacatctctt tgttttatac
     5581 cattactact atcgcttact tttattattt tttaccaacc ctgggtcagg gcattaccac
     5641 caacgccccg acacgctagt cctgagcaat tagaaaaaac agttcgttat cttacacaaa
     5701 ctgtgcatcc acgtagcgcc gacaatattg ataatctgaa taggtccgca gagtatataa
     5761 aagaggtctt tatcagtagc ggtgccagag ttaccgcgca ggatgtcccc attacgggtg
     5821 gcccctacaa aaacattgtt gctgattatg gtcctgccga tggaccgctg attattattg
     5881 gtgcgcacta tgactctgtc agtagttatg aaaacgatca attgacctat acgccgggcg
     5941 cggatgataa cgccagcggc gtggcaggat tactcgaact ggcgcgtttg ttacagctgc
     6001 agactccagg gattggcgtc caactggtcg cctatgcgtc ggaagaaccg cccttctttc
     6061 gtagcgatga aatggggagc gcggtacatg cagcttcgct tgagcgtcca gtgaaattaa
     6121 tgatagcgct ggagatgatt ggttattacg actcaacgcc tggtagccag gattacccat
     6181 ccccggcaat gtcctggctt tatcccgatc ggggagactt tattgccgtg gtcggcagaa
     6241 tgcaggatat caacgccgtt cgtcaggtaa aagcggcatt gttgtcatcc cgggatttat
     6301 ctgtttattc tatgaatgcc ccaggtttta ttcccggtat tgatttctct gatcacctta
     6361 attattggca acacgatatt cccgccgtaa tgattactga caccgctttt tatcgtaata
     6421 aacaatacca cttgcccggt gatatcgcag acagattaaa ttatcagaaa atggctcagg
     6481 tagtagatgg agttacaacc ttgttataca acagtaaata ataaaaattt ctcgaggcgg
     6541 atccagaaaa agcaacatgc gcattttatt ttctttcgtc tgttttaaga tgcgaaacat
     6601 gaaattcaac ggaggaaaaa taaatgggaa tgattggcta ttttgcagaa attgactcag
     6661 aaaagattaa ccaactgctt gagtctacta aaaaaccttt gatggataat atacatgaca
     6721 cgctttcagg attacgccga ctggatattg ataaacgctg ggatttttta cactttggcc
     6781 tgacgggtac atctgcattt gaccccgcga agaatgaccc gctaagtcgc gcagtgcttg
     6841 gtgaacacag cctagaagat ggcatagatg gcttcctggg attgacctgg aatcaggagt
     6901 tagccgcgac aatcgaccga ctggaagctc tggatcgaag tgagctgcgt aaacagtttt
     6961 ccattaagcg actcaatgaa atggagattt atccgggagt aaccttttca gaagagttag
     7021 aggggcaact ctttgcttcg atcatgcttg atatggaaaa gctgattagc gcgtatcgac
     7081 gtatgctgcg acaagggaat catgcgctga cggttattgt tgggtgatgg tgtcagggca
     7141 ccaaaaaaaa ccgccgaaca tgtcggcggc tttctgactt acaaactgac tgcttacatc
     7201 agcggcattg ctaaccgcac aatgctaatc agcggttgtg gccatacacc cagcaccagt
     7261 accaacagtg cagagatcag caccacgata ccgcctgcgc tgtactgcca gtttgatggt
     7321 gcatcgcgac ccggttgttc cggagcgtga aggtataagc tcaccgccac gcgcaggtag
     7381 tagtagaggc cgattgccga accgacaact acggcaccca ccagccacca caagtgtgcc
     7441 tggacaccga ccgccagcac gtagaactta ccgataaagc ccagcgtcat tgggatacca
     7501 gccagtgaaa gcatcatcac cgtcatcact gccgcgagga tcggacgatg ccagaacaga
     7561 ccgcggtaag agaacaggga atcagcatcc gggccacgat acgggctgga catcaggctg
     7621 accacgccga acgcgccgag gctgctgaac agataacctg ccaggtaaac ccctaccgct
     7681 tccatcgaca tctcgccagt ttgcagcgca atcagcgcca ccagcagata gccgaggtga
     7741 gagatagatg agtaaccgag cagacgtttg atattggtct ggctcagcgc catcaggtta
     7801 ccgaagatga tggaggcaaa ggcgataatc gccagcacca cgcgaatcgc ttcgctgtca
     7861 cccaccggtg cgtagaggaa cagacgcatc accacaccga agatagcgat tttgctcgcc
     7921 gtcgccagga aagtggaaac cggcgcaggc gcgccctggt atacgtctgg cgtccacagg
     7981 tggaacggca ccagagagag tttgaagccg aggccgacaa tcatcaggcc gaaacctgcc
     8041 agcaacagcg gctcgttgag cataccgtcg ccgaggtttt tacccagcgc gacaaacgac
     8101 aggtcgccag actgcgcata caccagcgcc ataccgaaca gcaggaaaga agacgctgcg
     8161 gcagagagaa tggtgtattt gatactggct tccagcgaac gtttctggcg gaaagcgtaa
     8221 ccgaccaggc caaacagcgg caaagagatc agttcgatac cgaggaacag agacgccaga
     8281 tggttggcat tcgccagcag gatcccgccc agggcggcaa ttaacaccaa caggtagaac
     8341 tcgtccttgt tgtcgttata gccttcaagc cacgggtagg cgaaagtaca ggtggcgagg
     8401 ctcgccaaca ataccagccc ggtgtaaagc atggcgaaac catcaacgcg catcagtggc
     8461 gtaacgtcca tagcgcccgc ctggccgaca aaccagagcg aaaccagcgc cgcgttaagc
     8521 ccaataaccg agagcgtagc gttgaggaaa tgattgcgtc gccacgcaat ggagagcatc
     8581 accaccacca ccgtcaagcc gacgatcagc aacggtagca gtgcgatcag gttttgtgga
     8641 gttattgtca tggcgattta cggccttgta gtagtaacgg aattaacaaa ccactgctgg
     8701 atattgccaa tcgcggagtg cgaggtatcc agaatcggct gcggatagaa gcccagcagt
     8761 accagcagca ccaccagcaa caggatcata aacagctcac gcagcgacat ccctggcagt
     8821 tcctggctgg caatctggct tttcgcttta ccgaagtaag cgcgatgtaa catcgccagc
     8881 gaataaacag atgcaaagac cagcccaaag gtagagataa cggtaatcac cgggacaacc
     8941 tggaagctgc cgaacagaat cataaattcg ccgacgaagt taccggtgcc aggcatccca
     9001 agcgttgcca ccgcaaagaa cagcgacagt gctggcagcc atttcatctt gctccacaga
     9061 ccgcccatca tgcgcatgtc gcgggtatgg atacgttcat aaagctgacc gcagagaata
     9121 aacagacccg ccgccgacaa gccgtgcgca atcatctgga ttaccgcgcc ctggtaggcc
     9181 aactggctgc cagtgtagat agcaatcagc acgaagccca tgtgggaaac cgaggtgtag
     9241 gcgatcagac gtttgatatc ggtctgggcg aaggccatcc acgcaccgta gaagatgccg
     9301 ataacaccca gccacatagc gattggcgcg aactctgccg acgcgttcgg gaacaacggt
     9361 agggagaaac gcagcagacc gtaagcggcg gttttcagca agatccccgc gaggtcaacg
     9421 gaaccggcgg ttggagcctg tgagtgcgca tccggcagcc agccatgcag cggaaccacc
     9481 ggcattttca ccgcaaatgc gatgaagaag cccagcatca acagatattc cacaccactg
     9541 gacattggcg tattcaacag ctcttcatag ttgaaggtcc agacgccggt cgcattgtag
     9601 tgaacaaaaa ccagcgccag gatggcgatc aacatcacca gaccactcgc ctgggtgtaa
     9661 atgaagaact tggttgccgc cgtgatacgc gttttaccgt cagaggcttt atgcccccac
     9721 agtgcgatca ggaagtacat cggcaccagc atcatttccc agaagaagaa gaacaggaac
     9781 atgtcgatgg caaggaacac gccgataacg ccgcccagga tccacatcag gttgaggtgg
     9841 aagaagccct gatatttttc gatctctttc cacgaacaga gtaccgccag cacaccgagc
     9901 agaccggtca gcacgaccat cagcagcgac agcccgtcaa tggcgagatg gatagagata
     9961 ccaaaacgcg ggatccacgg catgtcgaat tcagactgcc actgcggaat tccggcggat
    10021 tgcgtcagtg aataaccgcc ctgcaaccac agttgcagcg acagcgccag cgtcaatccc
    10081 atggtgatca gcgcgatcca gcgcggcacc ttgacgccaa agcgttcggt ctgccagcac
    10141 aggaagccgc cgataaaggg aattaatatt agccagggta gtaacatggc gatctttatt
    10201 ccttgtaaaa gtcccgtcag gaccggattt tcaacgaatt ctcacgacag aaaattctct
    10261 tcggactggg ggtagtgtcg gatgcaccgc gaatgcggct tatccgacct acgttcttat
    10321 atgcccaggg gcaatcccac aatccttaac tcaacgcagt accatcaaca gtgccagcac
    10381 cacgaccgca ccgatgctca tggatgccac ataccagcgc agataaccgt tctcgcttaa
    10441 cagcagacct ttacctgcaa agcgggaaag gacagccggg atgttcatca ttgagttcag
    10501 cggatcgcgt ttcagcaacc aggcaatacc caggaacggc ttgacgaaca ctttgtcgta
    10561 cagccagtca aagccccagg cgttgtacca ccaggtaccc agcagacggc ccggcgcact
    10621 gttggcgatg gaggtcacca gagtacgttt acccagccac agccaggcag ccagcagaat
    10681 gccgaccacc gcgaccacgc cagaggtaat ttccagggtc aacatgctgc cgtgcgccag
    10741 ttccgtcgtt tgcggaagca cgccctgcag cggcggtaca atcagtgcgc caacgaaggt
    10801 ggaaaggatc agcagcacaa tcagcggcag gctgtgagtt acccctttca cggcgtgagc
    10861 gtgaatttgt tcttttccgt ggaagacgat gaaaatcata cggaaggtgt agagcgaggt
    10921 cataaacgca ccgaccagac ctgccaccat cagattgata tgaccattcg ccatcgcacc
    10981 cgcgaggatc tcatccttac tgaagaagcc cgcagtgacc agcggcagtg ccgacagtgc
    11041 tgcgccgccc accaggaagc agagataaac cagcggaata gatttacgca gaccgcccat
    11101 cttgaagatg ttctgttcgt gatggcaggc cagaatgacg gaaccggatg ccaggaacag
    11161 cagcgcttta aagaacgcgt gggtcatcaa gtggaaaatc gccgcatccc atgcctgcac
    11221 gccaagcgcg aggaacatgt agccaatctg gctcatggta gagtaagcga gaacacgttt
    11281 gatatcggtc tgtaccagcg cggcaaaacc ggccagcagc agcgtaaccg ccccgacaat
    11341 gcccaccaga tgcagaactt ccggcgtcat caggaacagg ccgtgggtac gagcgatcag
    11401 gtagacaccc gcggttacca tcgttgcggc gtggatcagc gcggagacag gcgtcgggcc
    11461 cgccatcgcg tcggcaagcc atgtctgcaa cggcaactgc gcagatttac cgaccgcacc
    11521 gcccagcagc atcagcgtcg cccacatcag catgttattg ccgtcagcaa agtgcgctgg
    11581 tgccagttcc accatttcgc ggaagttcag ggtgcccagt tcgttgtaaa gaatgaacag
    11641 cgcgaaagcg aggaacacgt cacccacacg ggtcacgacg aacgctttca ttgccgctgc
    11701 gccattcttc ggatcggtgt aatagaaacc gatcagcaga taggagcaca ggcccacgcc
    11761 ttcccagccg aggtacatca acagtaggtt atcggcgaga accagaacta ccatgctggc
    11821 gatgaacagg ttggtgtaag cgaagaagcg agagtagccc tcttctccgc gcatatacca
    11881 ggaggcatac atgtgaataa ggaaacccac accagtgacc actgagagca tggtcagcga
    11941 caggccgtcc agcaccagat tgaaaccaat gttaaagtcg cctaccgaca tccacgtcca
    12001 cagcggctgg ctgtatgcct gctcaccgtt agcgaagaag tcaacgccga taaaggcggt
    12061 taccagcgcc gccaggccca cagagccaac gccgacgatc gccgagacgt tttcagacca
    12121 gcgcccacgg gagaacgcca gcaggacgaa gccaatcaat ggcaaaataa tggttaaggc
    12181 aagcatgttc atccgcgcat ctcacttact gaatcgatgt tcaggttctg gcgacgacgg
    12241 tgaagttgca gcagcagcgc aaggccgata ctcgcttccg ccgccgcgag gctgatggcg
    12301 agaatgtaca tcacctgacc gtcggtctgg ccccagtagc ttccggcgac cacaaaggcc
    12361 agcgcagagg cgttaatcat gatttccaga ccaatcagca taaacagcag attgcgacgg
    12421 ataaccagac cggttaagcc aagaacgaat aagattgccg cgaggatcag tccatgttgt
    12481 aaggggatca tgcgtgctcc tccgtttttc ttttcgcgct gtcgtcttta cgattgctca
    12541 gcacttcacc cgcacgctct tcacgaccga cgtggaaggc cacaaccaga cccgcgagca
    12601 gcagcataga agccagttcc accgccagta cgtaaggccc gaacagcgta atacccactg
    12661 ctttagcact gattggcgta ccgtcgatac cctgatcatt aacaccaagg atggcgtaaa
    12721 caatcaccac cagcatgatg gccgacaaaa tcgccggacc aatccacacc tgcggtttca
    12781 gccactggcg ttcctgttcg atttctgaac cgcccaggtt gagcatcatc accacgaaca
    12841 cgaacagcac cataatggca cccgcgtaga cgataatttc cagcgcaccg gcgaagtaag
    12901 cgcccagtga gaagaacacc ccagaaatcg ccagcagcga aataatcagg tacagcagcg
    12961 cgtgtaccgg attggtatgg gtgatcactc gcaaggtcgc aagtatggct atcaggccac
    13021 agatataaaa agcgaactcc attgcccctc tccttacggt aacaggctct tgacgtcgat
    13081 aggcttggct tcgttctctg cttcgccctt atctttgccg tcgattgcca tacctgccat
    13141 ccggtagaag ttatattccg ggtatttgcc cggaccggag atcagcagat cctctttctc
    13201 gtaaaccaga tcctggcgct tgtattcccc catttcgaaa tccggggtta actgaatcgc
    13261 cgtggtcgga caggcttctt cgcacagacc acagaaaatg cagcgtgaga agttgatgcg
    13321 gaaaaattcc ggataccagc gaccgtcttt ggtttctgct ttttgcagcg agatacagcc
    13381 gaccgggcag gctaccgcgc agaggttaca ggctacgcaa cgctcttcgc cgtccggatc
    13441 gcgggtcaga acgatacgac cacgataacg gggcggcaga tagaccggct cttccgggta
    13501 cattcgcgtt tcgcgtttgg cgaacgcgtg caggccgatc atccagatac tacgaacctg
    13561 ggtgccgaaa cctactaaca attcttttaa ggtcatggtc ttattgcccc ttattgcgcc
    13621 tgccagagaa tgacagccgc cgttaccagc aagttgatca gcgtcagcgg caggcagatt
    13681 ttccagccga aggacattac ctggtcataa cgcggacgcg gtaacgacgc acgaatcaaa
    13741 atgaacatca tcataaagaa cgcggttttc agcgcgaacc agatgaatgg cggtaacaac
    13801 gggccttgcc agccaccgaa gaacagcgtc accatcagtg cagagatggt cacaatcccg
    13861 atgtattcgc ccacgaagaa cagaccgaac ttcatgccgg aatattcaat gtggtaacca
    13921 tccgccagtt cctgctcggc ttccggctgg tcaaacgggt gacggtgaca caccgccacg
    13981 cccgcgatgg caaaggtaat aaaaccaaag aattgcggga tgacgttcca cacatgcgcc
    14041 tggctgttga cgatgtcggt catgttgaat gaaccggcct gcgccaccac gcccatcagg
    14101 gaaagcccga ggaacacttc gtagctcagg gtctgcgcag aagcacgcat cgcacccagc
    14161 agcgagtatt tgttgttact tgaccagccc gcaaacagca ccgcgtaaac cgccagaccg
    14221 gccatcatca ggaagaacaa aatcccgatg ttcaggtcgg caaccaccca acccggactg
    14281 actggcacaa tcgcaaaggc cagcagcagc gaggtaaagg caatcatcgg tgccagggta
    14341 aagatgacgc gatccgagaa tttcgggatc cagtcttctt taaagaacat cttgatcatg
    14401 tccgcgacca gctggagcga accgccccag ccaacacggt taggtccgta acggttctgg
    14461 aacagaccca gcaggcgacg ttcgccaaag ctcatgaatg ccccacaggt gacaaccacc
    14521 agcaggatca ccaccgcttt gaggatggtc agcaggatct caatcagttc cggtgatatc
    14581 caactcattg ttgtgcctcc ttgagatcct caagatgcgc gccagccagc accggagcaa
    14641 tgccggacat acccatcggc aagcccacct gccctgccgt cagtccttcg gcgatttcaa
    14701 ccggcagcgt gaccgtgttg ccatcgtaac taaaggagac gcgtgtaccc gcgttcacac
    14761 ccaacttcgc ggcatccgct gggttgagtt tgatgtacgg ctgcggcata cggctctgga
    14821 agaccggagc acgctgtgac aattcatcgc taccaaacag gtggtagtac ggcgcgatac
    14881 gccatttccc gtcctgcggc tggaagcgtg ccggtacgct ggtgaagtaa tccagaccat
    14941 tttcgctggt ttcaaacaga cgcacgcccg gatcgccaaa gcgcagtttg ccgcccactt
    15001 cgtcctggaa tttgttccac gcctgcgggg agttccagcc tggcgcccag gcaaacggca
    15061 cttgcgaacg gtgcgcggtc ggctggttgt taccttccat cgagaaggtg aacatggtgt
    15121 caatatcctg cggctgacgc ggctcatgaa cgctgatatt ggcgcgcatg gcggtacgac
    15181 cgctgtaacg gtgcggttca cgggccagtt tctgcccacg aatacggaat gtcgcatccg
    15241 gcgcagcatc tttgataccc gccagttccg ggattttcgc cacaacagcg tcaatcacat
    15301 ggtcgagctg cgtccagtcc acttcacggc tcagcaaggt gctgtgcagc gagtgtaacc
    15361 aacgccagct ttccagcatg acagttttgc tgtcgtaata agccggatcg taaacctgga
    15421 agaaacgttg ggcacggcct tcgttgttaa tcaccgtacc gtcgctttca gcaaagctgg
    15481 cagcagaaag taccagatgg gcgttttcca taatcgctgt gcgttgatgg tcaaccacca
    15541 tcaccagcgg cgctttagcc agcgcagcat tcacgcgggt agcggaagcg tgacgatgca
    15601 gatcgttttc cagcaccacc accgcgtcgg cgcgtccggt ttccagttcg gttaacgctt
    15661 cttcaagcga tccaccgccc ataatgccca gccccatgct gttgacggaa cgggcaatca
    15721 tggtgatacc gacgtcagcg ccgcgacctt tcagggcttt cgccacgtta gccgcagcct
    15781 gaatcacttc agcgctaccg gcgttcgtcc cggagataat caacggtttc ttcgcacctg
    15841 ccagtgcctg cacgatgacg tcgattttgc tttgcagctc aggttcaata ccgtcaaccg
    15901 ctggcgcgga gttatccagc gcatgggcga tggcaaaacc taaacgcgcc tgatcttcaa
    15961 ccggtgcgcg gtaagtccac gccgcgatat catccagacg ggtgtcatca acgttggtaa
    16021 caaacagcgg atgcttcgca cgttggccga tgttgaggat tgccgcaatc tgccagtcag
    16081 ccactttctg tgctgccgcc atttcgcgcg ctttaccttt caccgcctga cgaactgcca
    16141 gcgcgacgcg cgcgccggtc tgggtaacgt cttcgcccag caccagtacc gcatcgtaag
    16201 attcgatttc gcgcagtgcc ggcgtataaa tgccgccttc acgcagcact ttcagcgcca
    16261 gttgcagacg ttcctgctca ccgtgagcga taccggtgta gaagttttct tcgcccacca
    16321 gctcacgcag cgcaaagttg ctttccacgc tggcacgcgg agaaccaatg ccgatcactt
    16381 tcttcgactg acgcagaata tctgccgcgc cctgcattgc ctgttcggcg ttgagggtaa
    16441 tgaaatcatc gccacgacgc tgtactggct gacgcggacg atctttcagg ttgacgtaac
    16501 cgtaaccgaa acgaccacgg tcgcagagga agtagtggtt taccgtaccg ttgtaacggt
    16561 tttcgatacg acgcagttcg ccgtaacgct cgccggggct gatgttacag ccgatggaac
    16621 attgctggca gatgctcggc gcaaactgca tatcccattt acggttgtaa cgctcggagt
    16681 gcgttttgtc ggtgaatacg ccggtcgggc aaatctcgac cagattaccg gaaaattcgc
    16741 tttccagcgt gccgtcttcc gggcgaccga agtagacgtt gtcgtgcgca ccgtaaacgc
    16801 ccaggtccgt gccgtcagcg taatctttgt agtaacgcac gcagcggtag caggcgatgc
    16861 agcggttcat ttcgtgagag atgaatggcc ccaaatcctg attacggtgg gtacgtttgg
    16921 tgaaacggta gcgacggaag ctgtgtccag tcatcacagt catatcctga agatggcagt
    16981 taccgccctc ttcacatacc ggacagtcgt gcgggtggtt ggtcattaac cactcgacca
    17041 cactttcacg gaactgtttc gcttcttcat cgtcaatgga aataaaggtg ccatcggaag
    17101 ccggtgtcat acaggacatc accaggcgac cacgcgtgtc ttccgcgttt tggtattgct
    17161 tcaccgcaca ctggcggcaa gcaccgacgc ttcccagcgc cggatgccag caaaagtaag
    17221 gaatatcaag gcccagagac agacaagctt ccagcaagtt gtccgctccg ttgacctcgt
    17281 attctttgcc gtctacatga attgtagcca ttagcatgct tccagttttc tcagtcagag
    17341 actgagcgtt aatcgaaatt cggttaccag cgctctttca gcaggttcgg ctgaatccca
    17401 ttaatcaaat gggtattgct gaacggctgt ttgattcccg cctcaaattc ttcgcggaaa
    17461 tatttgatgg cgctctgtaa cggctccact gcaccaggtg cgtgggcaca gaaagtttta
    17521 cccgggccta agaatcgaca cagttgctca agtgtttcga tatcgcccgg ctgaccttcg
    17581 ccacgctcca gcgcacgcag aattttcacg ctccacggca gaccgtcgcg gcacggcgta
    17641 caccagccac aggactcacg ggcgaaaaac tcttccaggt tacgcaccag cgacaccatg
    17701 ttgatctcat ggtcaaccgc catcgccagc gccgtaccca gacggcttcc cgctttaccg
    17761 atactttcga attccatcgg cagatcaagg tgcgcttcgg tcaggaagtc ggtgccagcg
    17821 ccgcctggct gccaggcttt gaatttcaga ccgtcacgca taccaccggc gtaatcttcg
    17881 aggatctcgc gtgcggtggt gccgaatggc agttcccaca ggcccggatt tttcacccga
    17941 ccggagaagc ccatcagctt ggtgccagca tctttacttt tcgagatgtt ctgataccac
    18001 tccacgccgt tagcgaggat cgccggaacg ttacacaggg tttcgacgtt attgacacag
    18061 gtcggtttgc cccatgcacc ggaggttgcc gggaatggag gcttcgagcg agggttagcg
    18121 cgacgccctt ccagggagtt gattaacgct gtttcttcac cgcagatgta gcgcccagcc
    18181 ccggtatgga cgaacaattc gaagtcgaaa cctgttccca taatgttttt gccaagcaga
    18241 cccacttcgg tggcttcggc aatggcacgg cgcagattca ctgccgcttc gatatactcg
    18301 ccacgcagga agatgtagcc acggtacgct ttcagcgcaa acgcggagat gagcatacct
    18361 tccaccagca ggtgcggcag ttgttccatc agcaggcggt ctttataggt gcccggctcc
    18421 atttcatcgg cgttacacag caggtaacgg atgttcatgg attcgtcttt cggcatcagg
    18481 ctccatttca ggccggtaga aaagcccgcg ccgccgcgcc ctttcagacc agcgtctttt
    18541 acctgattaa cgatttcgtc cggagacagc ccggtcagcg ccttacgcgc gccttcgtaa
    18601 ccgtttttgc tgcgatattc gtccagccac actggctgtt tgtcatcgcg cagacgccag
    18661 gtcagcggat gcgtttcggg agtacggata atgtttttca tttataccgc tccagcagtt
    18721 cagggatcgc ttccggggtc agatgcgcgt gagtgtcctc atcgatcatc atgtttggcc
    18781 ctttgtcgca gttccccagg cagcaagttg gcagcagcgt aaagcggcca tcaaatgtcg
    18841 tttgccctgg tttgatgttc agctttttct ccagcgccgc ctgaataccc tggtaaccgt
    18901 tgatgtgaca gaccacgctg tcgcaataac ggatcacatg gcgaccaacc ggctggcgga
    18961 agatctggct atagaacgtt gccacgcctt cgacgtcgct cgccggaata cccagcacat
    19021 cggcgatcgc gtggatcgcg ccatccggca cccagccacg ctgcttctga acgattttca
    19081 gcgcttcaat ggacgccgca cgcgggtctt cgtagtggtg catctcgtgc tcaatcgctt
    19141 cacgctctgc cgcactcagc tcaaaagcct cggtttgtgg ttgttgattc tcgtgcataa
    19201 ttagcggtcc acatctgaca taacaaaatc gatactgccc agataaacaa tcaggtcaga
    19261 caccaggctg ccgcggatcg ccgccggaat ttgctgcaaa tgcgcataac tcggcgtacg
    19321 gatacgggtg cgataactca tggtgctacc gtcgctggtc aggtagtaac tgttgatccc
    19381 cttggtcgcc tcaatcatct ggaaagattc attggctggc atcaccggac cccacgacac
    19441 ttgcaggaag tgggtgatca gggtttcgat atgttgcagc gtgcgctctt tcggcggcgg
    19501 cgtggtcagc ggatgatccg ctttgaacgg gccttccggc atgttgttga ggcactgctc
    19561 aagaatgcgc agactctggc gcagctcttc cactttcagc attacgcggg tgtagcagtc
    19621 agaaacacca ccacccaccg ggatttcaaa gtcgaagttt tcatagccag aataaggacg
    19681 cgccttacgc acgtcgaagt cgatcccggt ggcgcgcagg cccgcgccag tggtgcccca
    19741 ctccagcgcc tctttcgcgc cataggcggc aacgccctgg gaacgacctt tcagaatggt
    19801 gttttgcagc gccgctttct cgtaagacgc cagacgtttc ggcatccagt cgaggaactc
    19861 acgcagcagg cgatcccagc cacgcggcag gtcgtgcgct acaccgccaa tacggaacca
    19921 cgccgggtgc atacgaaaac ccgtgatcgc ttccaccaga tcgtaaattt tctgacgatc
    19981 ggtaaaggcg aagaacactg gcgtcatcgc gccgacgtcc tgaataaagg tggagatata
    20041 cagcaggtga ctgttgatgc ggaacagttc ggagagcatt acgcgaatga cgttaacgcg
    20101 atccggcacg gtgatcccgg ccagtttctc taccgccagc acgtaaggca tttcgttaac
    20161 gcagccgccg aggtattcga tacggtcggt atacggaatg tagctgtgcc aggactggcg
    20221 ttcgcccatt ttctccgcac cacggtggtg gtagccgatg tctggtacgc agtcgacaat
    20281 ttcttcgcca tcaagctgca aaacgatgcg gaaagccccg tgcgccgacg ggtggttcgg
    20341 gccgaggttg aggaacatga agtcctcgtt ttcggtgccg cgcttcatcc cccactcttc
    20401 cggtttgaag gtcagggctt ccatctccag gtcctgtttg gctttggtca gctcaaacgg
    20461 cgagaattcg gtagcgcgcg ccggataatc tttacgcagc gggtgacctt tccaggtttg
    20521 cggcatcatg atgcggcgca ggttcgggtg accgtcgaaa gtaatgccaa acagatccca
    20581 ggtttcacgc tcataccagt tagcgttcgg gaacagtttg gtgaaggtcg gtacgtgcag
    20641 gtcgttttct gccagcgcca ccttcagcat gatgtcgcgg ttacgatcga tagaaatcag
    20701 atggtagaaa acggaaaaat ccgcggcagg taacccttcg cggtgtgtgc gcagacgttc
    20761 gtccatgccg tgtaagtcaa acagcatgac gtaaggtttc ggcagtttct ttaagaaatc
    20821 gcccacttcc agtaattgtt cacgcttgat ccacacaacg ggaaccccgg tgcgagtcgc
    20881 ctgaacagta aaggcatccg gcccaaaacg gttgcgcagt tcgccaatca ccggatcatc
    20941 aagatgatcg cgggtctgcc aggcgggttc ttgcgcggtt aagtcggtca tattgttcac
    21001 cattgcaaat ggtccgtggt gactgtcggc gcagtaagct tcgcgaaata gatttaggaa
    21061 taagcgaaga aaaattcctt tgccgacagg cgcaaattaa atctcgtcag gtgtacgcag
    21121 gttggttacg gcaatgcgtt caccgcgctt gcgttcgcgc tctgattgca tattggcgcg
    21181 ataaacgccc tgatcgccaa ccacccagga gagcggacga cgttctttac cgatagattc
    21241 ctgcaacagc atcagtgcct gcatgtacgc ttcaggacgc ggagggcagc ccgggatata
    21301 cacatcaacc gggatgaatt tatcgacgcc ctgcacaacg gaataaatat cgtacatacc
    21361 gccggagttg gcacaggcac ccattgagat aacccatttt ggttccagca tctggtcata
    21421 cagacgctga ataaccggtg ccatttttgt aaagcaggtt cctgcaacga ccatcaggtc
    21481 agcctgacgc ggcgaagctc gcaatacttc tgcgccaaaa cgcgccacgt catgcaccgc
    21541 ggtaaacgaa gtcaccatct caacgtaaca gcaggaaaga ccgaagttat acggccaaat
    21601 tgagttttta cgaccccagt taaccatgtc attgagcttg cccataaaca cgtttttatt
    21661 aacttcttgc tccagagggt cggttacgat ctcctgcttt tgcagggggt aacggtcgtt
    21721 ctcaccgttg ggatctatgc gggtgagcgt ataatccatc ttaatgcctc gcggttagcg
    21781 ttgacgatta gcgatactgt tcgtttccgg gttcatacgc tcgcggcgtg aacgcgcggg
    21841 cgtccagtcc agcgcgccaa tacgcaccag ataaaccaga cctgccagta acacaaaaat
    21901 aaaaattgca gcttccacaa agcctaccca gccgctttcg cggatagagg ttgaccatgc
    21961 gaacagatac agcgcttcaa cgtcgaagat aacgaagaac atggccacca gataaaactt
    22021 ggcggacagg cgtaagcggg cggagccgac cgagtcgata ccggattcaa acggaacgtt
    22081 tttcgacctt gcgcgtgcgc gaccgcctaa aaaccaaccg cctaccagca tcaggcaaca
    22141 caggccaatg gcaacgataa gaaagatagc gaatgcccag tgatgagcga tgacttcagt
    22201 ggatgttgac atactcattg cttactcatc aaaagtagcg ccagattcac tgctcttcac
    22261 ggcagatgga cgccacatcg attcatgggg aggaataaaa aaaaccttac aatcactgta
    22321 gaaattcttt tatacagcta attgatgtgg tcttttactc ctttctataa ccttttgtca
    22381 actttaacaa aagtttcttc acattagttt acataatatc aacaccatta gcatttaatg
    22441 ccctttcacc ccagatcctt gacgactcca ggataattag atgttgttga atcgtgtccg
    22501 ttgtgaagca atggaaaaaa tacgggtcta ttttgacagg aatttgtgtc gattcctccc
    22561 cccaaaagag agtattttct tgatctgtga cacgcttttg tcattccata acaaaaacgc
    22621 agcaacaaat ttacgtattt tttaacatca ttgtagcagg tgattttttt caggcgatta
    22681 tttgtgcgtt cgggacgtga atctctggtg attgaaaaat gaacagtttt gtacgttctg
    22741 cacgatacga ggaaggcttt ttactaaaaa aaagccgctg gggtttaaaa cacccccagc
    22801 ggctcgtttt tacactattg tctcagaaat tatctatggt ccgtcgatta ctcgatatcc
    22861 ctttcaatca acaatgagtc atcgccttcc ggagcagaca ttgggctgta ttgccacgga
    22921 ttatggtagc tctccatcgc ctgatagatc acctgggcca gctcattatt actggacggg
    22981 tcatagcaca gcaaatattc ggtatcgggt agcggcggta agccatctac tccactcagc
    23041 acacgcaggt ctggactcat catctcgacc ggccttgccg tcacgccaag accggctttt
    23101 actgccgcac gaactgccgg aagcgtcgag gcgacataag ccagtcgcca tggaatatct
    23161 gctttattaa gcgtcgccag caccatatcg cgaaacgggc taggatcatc cagcaataca
    23221 agagggatcg gctcgccttt ttgcaatacg tattccgctg cgcagtacca gtgtgttggc
    23281 gaggtacgca ggttcaatgc cttaaacgtt gaaggacgat gggtggttac catcaaatcc
    23341 acttcctggg attccagcat ttctgccata taggcattac gtttaacgcg gacatccagc
    23401 gctagtttcg gataaaccga actcacgcga tttaacagga aaggtaagat cgtatcggca
    23461 gattcatctg aagcaccgat agttaacacg ccctgaagat tactgaacat taatgatgag
    23521 caggcctcat cattaaaacg cagaattttt ctggcgtaac caagaagttg aatgccatgt
    23581 tcagttaaca gtttgttgcg accgtgacga gcgaacagtt ctttcccaac gagttgttcc
    23641 agacgctgca tttgctgact tacggcggac tgagtacgac acacagcggc agctgcggca
    23701 gcaaaagtgt tcagatcggc aacagcaaca aatgttctca gcagatcgag gtcgaggtta
    23761 attatcggac gatttgcact tatcatatat tatcacttac tggcggctca tactgagctg
    23821 gttaatgctg tgcacacaca aacaagcaat tccatttgta atgtgcctcc ctggcagttt
    23881 catccggaac ccggacgaaa gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca
    23941 ttgttccgtt ataatgcctg aagtagatca cagaatatat cttcaggaat cgcatatcta
    24001 ttaagttact cactcttttg tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga
    24061 gtccttgttc cactctcgtg cagcatcgct ggtcatacgc gaacacgtac aaacagcaat
    24121 ggtgaggcgg catcaagagc agggatccgt catttatgcg agcattttac cccaaaaacc
    24181 ttttatt
//
LOCUS       DAZKJR010000075        24110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKJR010000075 DAZKJR010000000
VERSION     DAZKJR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.075"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..1558
                     /gene="pitB"
                     /locus_tag="ACHOWS_002989"
     CDS             59..1558
                     /gene="pitB"
                     /locus_tag="ACHOWS_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFQ0048533.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1607..2299)
                     /gene="yghT"
                     /locus_tag="ACHOWS_002990"
     CDS             complement(1607..2299)
                     /gene="yghT"
                     /locus_tag="ACHOWS_002990"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0048534.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            2473..3186
                     /gene="yghS"
                     /locus_tag="ACHOWS_002991"
     CDS             2473..3186
                     /gene="yghS"
                     /locus_tag="ACHOWS_002991"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0048535.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3218..3976
                     /locus_tag="ACHOWS_002992"
     CDS             3218..3976
                     /locus_tag="ACHOWS_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048536.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4022..5371
                     /locus_tag="ACHOWS_002993"
     CDS             4022..5371
                     /locus_tag="ACHOWS_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFQ0048537.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5371..6195
                     /locus_tag="ACHOWS_002994"
     CDS             5371..6195
                     /locus_tag="ACHOWS_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048538.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPV"
     gene            6207..6767
                     /locus_tag="ACHOWS_002995"
     CDS             6207..6767
                     /locus_tag="ACHOWS_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFQ0048539.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLRNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6797..7867
                     /gene="lptF"
                     /locus_tag="ACHOWS_002996"
     CDS             6797..7867
                     /gene="lptF"
                     /locus_tag="ACHOWS_002996"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFQ0048540.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7864..8943
                     /gene="lptG"
                     /locus_tag="ACHOWS_002997"
     CDS             7864..8943
                     /gene="lptG"
                     /locus_tag="ACHOWS_002997"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFQ0048541.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVDVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETQ
                     ETSDNLPWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(8978..10150)
                     /locus_tag="ACHOWS_002998"
     CDS             complement(8978..10150)
                     /locus_tag="ACHOWS_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0048542.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTSYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTISTQPELRQKLMDNANNLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10150..10398)
                     /locus_tag="ACHOWS_002999"
     CDS             complement(10150..10398)
                     /locus_tag="ACHOWS_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0048543.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10430..11344)
                     /locus_tag="ACHOWS_003000"
     CDS             complement(10430..11344)
                     /locus_tag="ACHOWS_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0048544.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLIAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLNAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAEARCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11341..13065)
                     /locus_tag="ACHOWS_003001"
     CDS             complement(11341..13065)
                     /locus_tag="ACHOWS_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFQ0048545.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARSEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
     gene            13437..14579
                     /gene="yghO"
                     /locus_tag="ACHOWS_003002"
     CDS             13437..14579
                     /gene="yghO"
                     /locus_tag="ACHOWS_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFQ0048546.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHYGMIDAIDD
                     PQVFAALFGAAEAWLKSQGANKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(14586..15350)
                     /gene="glcC"
                     /locus_tag="ACHOWS_003003"
     CDS             complement(14586..15350)
                     /gene="glcC"
                     /locus_tag="ACHOWS_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFQ0048547.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            15601..17100
                     /gene="glcD"
                     /locus_tag="ACHOWS_003004"
     CDS             15601..17100
                     /gene="glcD"
                     /locus_tag="ACHOWS_003004"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFQ0048548.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            17100..18152
                     /gene="glcE"
                     /locus_tag="ACHOWS_003005"
     CDS             17100..18152
                     /gene="glcE"
                     /locus_tag="ACHOWS_003005"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFQ0048549.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            18163..19386
                     /gene="glcF"
                     /locus_tag="ACHOWS_003006"
     CDS             18163..19386
                     /gene="glcF"
                     /locus_tag="ACHOWS_003006"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFQ0048550.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGVEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASADRTS
                     VRHWIEIVEQALEKE"
     gene            19391..19795
                     /gene="glcG"
                     /locus_tag="ACHOWS_003007"
     CDS             19391..19795
                     /gene="glcG"
                     /locus_tag="ACHOWS_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFQ0048551.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            19817..21988
                     /gene="glcB"
                     /locus_tag="ACHOWS_003008"
     CDS             19817..21988
                     /gene="glcB"
                     /locus_tag="ACHOWS_003008"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFQ0048552.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDTAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPSCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPHEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            22344..24026
                     /gene="glcA"
                     /locus_tag="ACHOWS_003009"
     CDS             22344..24026
                     /gene="glcA"
                     /locus_tag="ACHOWS_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFQ0048553.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         5704 a         5971 c         6496 g         5939 t
ORIGIN      
        1 ccgttaaagt cactgataat gcgtccgttc gtaaattcaa aatggcgtaa tctaatatat
       61 gctaaattta tttgttggcc ttgatatata cacagggctt ttgttattac ttgctctggc
      121 atttgtgttg ttctacgaag caatcaatgg ttttcatgac acggcgaatg cggtggcaac
      181 cgttatttat actcgagcca tgcagccaca gcttgccgtg gtgatggcgg cattttttaa
      241 cttttttggc gtattgctag gcggacttag cgttgcctat gccattgtcc atatgttacc
      301 aaccgatttg ttgctgaata tggggtcaac tcacggcctg gcgatggtct tttccatgct
      361 gctggccgcg attatctgga acctgggaac gtggttcttt ggtttaccgg cctccagttc
      421 gcacaccttg attggtgcga ttatcggcat cggtttaacc aacgcgctgt taaccggctc
      481 atcggtgatg gatgcgttaa acctgcgtga agtgaccaaa attttctcct cgctgattgt
      541 ttcccctatc gtcggcctgg tcattgcggg tggtctgatt ttcctgctgc gccgtttctg
      601 gagcgggacg aaaaagcgtg accgtattca ccgcattccg gaagatcgca aaaagaaaaa
      661 aggcaaacgt aagccaccat tctggacgcg tattgcgctg attgtttccg ctgcgggcgt
      721 ggcgttttcg cacggtgcta acgacgggca gaaagggatc ggcctggtga tgctggtact
      781 ggtggggatt gcccctgctg gtttcgtcgt caatatgaat gcgtccggct atgaaattac
      841 ccgtacccgc gatgccgtca ccaacttcga acactacctg caacagcatc ctgaactgcc
      901 gcagaagttg attgcgatgg aacctccatt gcctgcggca tcgactgatg gcacgcaagt
      961 aacggagttt cactgtcatc cggcaaatac ctttgatgcg attgcgcgcg ttaaaacgat
     1021 gctgccaggc aatatggaaa gttacgagcc gttaagcgtg agtcagcgca gccagctgcg
     1081 ccgcattatg ctgtgcatct ctgatacctc cgcgaagctg gcgaaactgc caggcgtcag
     1141 taaagaagac cagaacctgc tgaaaaaact gcgcagcgat atgttaagca ccattgagta
     1201 cgctccggtg tggatcatca tggcagtagc tctggcgctc ggcattggca ccatgattgg
     1261 ctggcgtcgt gtagcgatga ccatcggtga gaagattggt aagcgcggca tgacgtatgc
     1321 gcaaggcatg gcggcacaaa tgacggcggc agtgtctatc ggccttgcca gttatattgg
     1381 gatgcccgtc tccacaacac acgtcctctc gtctgcggta gcagggacga tggtggtgga
     1441 cggcggtggg ttacagcgta aaacggtaac cagcatcctg atggcgtggg tgtttacttt
     1501 accggcggcg atttttcttt ctggtgggtt gtactggata gcattgcagt tgatttaacc
     1561 ttcctgaaaa tgcccggtcc tggcgatcgg gcatttccat ttttgactag tgataaccac
     1621 gcgcggtcat aaaatccgta atcgcttttt ctgcatcaac cagcaccttt tccagtggct
     1681 gattggcgtc gatatcaacc agttgtgcac cgccaaaggt taactgtggc gttatggcaa
     1741 tcttcctcgc cagcgattcc cgtttatggt cgggtttacg cgcgcaggca acttcaaggt
     1801 caacattgag tttgatgacc agatcaggct tatggctcgc catccagtga aatgctttac
     1861 gttcctggct tgccagccat gagacaaacc ggccgccttc aacgttaggt gggaacaccg
     1921 taccatcgta agcgccagga atttggtcct gaggataacg atcggttaag acaatcagcc
     1981 cgcgacgacg acaggcgagc atatgacgaa agcgtaacag tcggcgggcg acgaaagcgg
     2041 tgatcaccag ggccggaacc ggacccggca atttttttgc cgttttcact tcattttttt
     2101 caatagtctt gtgcagagac tttcccatca acggtaattt tgtcactgca cgaccgacat
     2161 ttccggcctg ttttcctaaa tgaactcttt cggcagcacc atatttttcg acaacggtaa
     2221 taagatgttc acacaccgtt gacttgcctg aaccatcgct accaataacg gcaattaatg
     2281 gaggtgttat tgactgcatg aatatatcct taaatttaaa tatccatcca aaatatttat
     2341 ttgattaata tgtttttatg aaagcgtaat tcaggttaat gtcacaatta accattgtca
     2401 caataaggtt gaacggatat tctgagcacg gaccacgtaa tattcaatac attatttgct
     2461 gccgggtttt tcgtgaatca acgtaatatg tcaataatta attccacgcc agtgcgtgtt
     2521 attgccattg taggatgtga tggttcaggt aaatcgaccc tcacggcaag cctggtaaat
     2581 gaactggcag caacaatgcc aacagaacac atttatctcg ggcaatcgtc cgggcgaatt
     2641 ggcgaatgga tttcacagct ccctgttatt ggcgcacctt ttgggcgtta tctgcgaagt
     2701 aaagcggcac atgtgcacga aaagccctcc acaccacctg gcaatattac tgcgctagtt
     2761 atctatctgc tttcctgctg gcgggcgtac aagtttcgca aaatgttgtg taaaagccag
     2821 caaggctatc tgctcatcac cgaccgctac ccgcaggttg aagtgccggg ttttcgcttt
     2881 gatggcccgc aattggcaaa aaccacgggc ggtaacggtt ggataaaaat gttaaggcag
     2941 cgcgagctga agctgtacca atggatggcg tcttatttgc ccgtattgtt gattcgtctt
     3001 ggcattgatg aacaaaccgc gtttgcgcgt aaacctgacc accaactggc ggcgttacag
     3061 gaaaaaatcg ccgttacgcc gcaactgaca tttaatggcg caaagatcct tgaactggat
     3121 ggtcggcaac ccgccgatga aattctgcaa gcgtcactac gcgcaattca cgccgcgctt
     3181 tcctgatcac caaaatggat aggggatgat ttaacgaatg gacgcactac aaacacaaac
     3241 tgttaatagt acaaccgcgc cgcagcccaa ctacattccg gggctgattg cggtggtcgg
     3301 gtgtgatggc accggtaaat ccacactgac caccgacctg gtgaaatcgc tgcaacaaca
     3361 ctggcaaacc gagcggcgct atctggggct gctctcgggc gaagacggcg acaaaatcaa
     3421 acgattgccg ttggttggcg tctggctcga acggcgactg gcggccaaat cctcgaaaac
     3481 ccaaagcatg aaaaccaaat ctccagcgct atgggcggcg gtgattatgt actgcttctc
     3541 gctgcgaaga atggcgaatc tacgcaaggt tcagcgactg gcgcaaagtg gcgttctggt
     3601 ggtcagcgat cgcttcccgc aggctgaaat ttcgggcttt tattatgacg gaccggggat
     3661 cggcgtcgaa cgtgcgaccg ggaaaatcag catgtttctg gcgcagcgcg aacggcgttt
     3721 ataccaacaa atggcgcaat accgtcctga attaatcatt cgtttgggga ttgatattga
     3781 tactgccatc tcccgcaagc ctgaccatga ctatgccgag ctgcaggaca aaatcggcgt
     3841 catgtcgacg attggctata acggcacaaa aattcttgag attgattcca gagcgcccta
     3901 cagcgaagtg ctggaacagg cacagaaggc ggtttctctg gttgccatcg tttctgaccg
     3961 ccgaagttta acttaaggct ggcgggatac tttttactat caacgataag gtcaggctga
     4021 attggcgggt tttaacatca aacattggtt tgcagatggc gcgtttcgca ccattattcg
     4081 caatagcgcc tggttaggct ccagtaatgt cgtgagcgct ttgctgggtc tgttggcgct
     4141 ctcgtgtgcc ggtaaaggga tgacgcccgc catgtttggc gtactggtga ttgtacaatc
     4201 gtacgccaag tcgatcagcg attttattaa gtttcagaca tggcagctgg tggttcagta
     4261 cggaacacca gcattaacca acaataatcc gcagcaattc cgcaatgtcg tctcattttc
     4321 cttctcgctg gatatcgtca gcggcgcggt ggcgattgtc ggcggcattg ccttactccc
     4381 ttttctttcc cattcattag gtctggatga ccaaagcttt tggctggcag cgctgtattg
     4441 cacgctcatt ccttcaatgg cttcctccac gccgaccggc attctgcgtg cggtagatcg
     4501 cttcgatcta attgctgtac agcaagcgac gaaacctttt ctgcgcgcag cggggagcgt
     4561 cgttgcctgg tattttgact ttggttttgc gggttttgtt attgcctggt acgcctcgaa
     4621 tcttgttggc ggcaccatgt actggtggtt tgccgcacgt gaattacgcc gcagaaatat
     4681 ccataacgcg ttcaaattga atctgtttga gtctgcccga cacatcaaag gcgcgtggag
     4741 ttttgtctgg tcaaccaaca ttgcccattc catctggtcg gcgcgtaatt cgtgcagcac
     4801 agtgttggtg gggatcgtgt taggacccgc tgccgccggg ttatttaaaa tcgccatgac
     4861 attcttcgac gccgccggaa cgccagcggg tttactgggc aaaagttttt acccagaggt
     4921 gatgcgttta gatccgcgta ccaccagacc gtggttgtta ggcgtgaagt caggtttact
     4981 ggcgggcgga atcggtattc tggtggcgct cgcggtgttg attgtcggca agccgctcat
     5041 ttcgctggtg tttggcgtta agtatctcga agcgtatgac ctgattcagg tgatgctggg
     5101 cgcaattgtg atctcgatgc tgggcttccc tcaggagtca ttgctattga tggcgggaaa
     5161 acagcgcgct tttctcgtgg cgcaaaccat cgcctcaatc ggctacatcg tattgctgtt
     5221 catgttctgt catctgtttg gcgtgctggg agcagcgttt gcttatttcg gtggtcaatg
     5281 tctggatgtg gtgctttcgc taattccgac cttaaaggca ttttttcagc gccattcctt
     5341 gctttataac gcagccggag agaaatcctg atgatgagaa aatatttccc gctagaggcg
     5401 agcgagcgtc tatttgttgc gattgaagaa gatgatgtcg ttgatgcaca ggtcagtttg
     5461 ccgccgacta tcgcactaag ttgtaccacc gaaatcatcc atgataatta cgcgttatgc
     5521 ctgcaattct ggctcaatgg cgtcaatcgc caggagttac tgcgtttaat atgcaaacag
     5581 gccaaaggcg atgagctgac tgctgatgag cgcaaacaat ttaaatacat gcgagcgcgc
     5641 tacaagcacc tgcgttttgc gcaacggctg tatttaaaga agcatcaggc cggattttta
     5701 ttcggcaaaa cgaccgtttt tttggggcgt tttcaggacg gctttcgtaa cggtaagaaa
     5761 aatattgtgt cgtattacgg caacctgtta cgggtttatt taagctctcc ggtctggtcg
     5821 ttggtgaact attcattacg ccatagccag ttagaaagcg taagcggttt tattgcctat
     5881 cggcaaaagc aaatgcatac cctgaaagag ataattgcca aaccgcgact aaccggcagg
     5941 gaatttcatg atgtgcgaaa aatcatcagc cagcaggttt cttattacga caccctaaga
     6001 tcgcttgatc ctgaaaataa agaagcgtta cagatttccc gcttcctcgc cgccattaat
     6061 ggcctgatgg gggataaaca cgacgacatg gtggcggatg atatggaaaa ccgccagtcg
     6121 tatgatgcac cgatggcgct ggatagcgat attcgccagc gcttagaact gctgatttcg
     6181 cgttttccgg tataaccgga gcaatgatgt catctcgttt gattattgcg ctgattatca
     6241 tgctcttagc cccgggtgtg caggcacaca atttcgtcac tggcaaaacg gttacccccg
     6301 tttatatcca ggaaggcggc gagctgcttc ggaatagcga cgacgaaatt cactaccaaa
     6361 agtggaacag cacccaattg gcgggaaaag ttcgcattat tcagtatatc gccgggcgta
     6421 agtcggcgaa gaaaaagaac tcactgttaa ttaaagcagt tgaagcggca aactttccgc
     6481 aggatcgttt tcagcccacc accattgtga acactgatga tgccatcttt ggtaccggct
     6541 attttgtcgt tggcaaaatc gaaaaaaata aacgccgcta tccatgggcg caattcgtta
     6601 ttgacggcaa tggacaggga cgcgttgcct ggcacttgca ggagcagagt tcaaccattc
     6661 tggtgctgaa taaaaccggg caaattcagt gggcaaagga cggcagcctg acgccggaag
     6721 aggtagatca tgtcattgcg ctggcgcaga agctgatcaa tgaataaaaa ttcaaaggat
     6781 tgagggcgtt agtgagatga agctggttga acactacatc atgcgtggta cgcgccgtct
     6841 ggtgctgatt atcgtcggtt ttctgatctt tattttcgcc agctactccg cacagcgtta
     6901 tctgaccgaa gcggcaaatg ggacattagc tcttgatgtt gtgctggata tcgttttcta
     6961 caaagtgctg attgcactgg agatgttgtt acctgttgga ctgtatgtgt cagttggcgt
     7021 gacgctaggg cagatgtata ccgactcgga aattaccgct atctctgcgg cggggggcag
     7081 tccgggacgc ttgtacaaag ccgttcttta tctggcgata ccgctaagta tttttgtcac
     7141 ccttctgtcg atgtatggtc gtccgtgggc ttatgcgcag atttatcaac tggagcaaca
     7201 gtcacagtcg gagctggatg ttcgccagtt gcgggcaaag aaatttaaca ctaacgataa
     7261 cggacgaatg atcctttcgc agacggttga tcaggataat aatcgcctga ctgatgcgct
     7321 gatttatact tctactgcta atcgaacccg cattttccgc gcccgttcgg ttgatgtggt
     7381 tgacccatca cctgagaaac cgaccgttat gttgcataac gggaccgcct atcttctcga
     7441 tcatcagggg cgtgacgaca acgaacagat ctaccgtaat ctgcaattac atctgaatcc
     7501 gctggatcaa agccctaacg tcaaacgcaa agcaaaatcg gtcacggagc tggcgcgctc
     7561 cgcctttcct gccgatcatg ccgaactgca atggcgacaa agccgtggcc tgacagcatt
     7621 gttgatggcg ctgctggcca tttcattaag tcgggtaaaa ccgcggcaag ggcgattttc
     7681 aacgttattg ccactgacgt tgctgtttgt tgccattttt tatggcggcg acgtctgccg
     7741 tacgctggtg gctaacggcg cgattcccct cattcctggt ttgtggttag tacccggact
     7801 catgctaatg ggcctgctga tgttggtcgc acgcgacttc tctttgctgc agaaattttc
     7861 ccgatgaatg ttttcagtcg ctatttaatc cgtcatctct ttctcggttt tgccgccgcc
     7921 gcagggctat tgctgccgct ttttaccacc ttcaacctga ttaacgaact ggatgatgtc
     7981 agcccgggtg gttatcgctg gactcaggcg gtgctggtgg tgctgatgac cttaccgcgc
     8041 acactggtcg aactttcgcc gtttatcgcc ttattgggag ggattgtcgg cctggggcag
     8101 ttatcgaaaa acagtgagct taccgccatt cgcagcacgg ggttttctat cttccgtatt
     8161 gcactggtgg cgctggttgc cgggatattg tggactgttt cattaggcgc gatagatgag
     8221 tgggtggcgt cgccattgca gcaacaggcg ctgcaaatca aatcgactgc cactgcgttg
     8281 ggggaggacg atgacattac cggcaatatg ctgtgggcca ggcgtggtaa tgaatttgtg
     8341 acggtgaaat cgctgaacga gcagggccag ccggtggacg tggagatatt tcattaccgc
     8401 gatgatctct cgctcgaatc ctacatttat gcgcgcagtg ctaccattga agacgacaaa
     8461 acgtggatcc tgcatggtgt gaatcataaa aaatggctga atggcaaaga aacgcaggaa
     8521 acatctgata atcttccctg gcaatcggcc ttcaccagta tgaatcttga agagttatcg
     8581 atgccgggaa atactttttc tgtccgtcaa cttaatcatt acatccatta tttgcaggaa
     8641 accgggcaac ccagcagcga ataccgcctt gcactgtggg aaaaactggg acaacccatc
     8701 ttgaccctgg cgatgatttt gctggctgtg ccgtttacct ttagcgcccc gcgttcgcct
     8761 gggatgggta gccgtctcgc tgtaggcgtc atcgttggct tactcacctg gatcagctat
     8821 caaatcatgg tcaatctggg attgttattt gcgttgagcg cacctgttac cgcgctcggt
     8881 ttaccggtag cgtttgtgct ggtggcgttg agcctggtgt attggtatga cagacaacat
     8941 taaacccgcc gtggcgcaac accacagcgg gctcacatta ctgcttcaga gtagcaaatg
     9001 cctgaatgat ggcatcgatt tgctcacgac tgtgggcagc gtttacgcta cagcgcagca
     9061 gcgtgatgcc tgcaggcgcg gcaggtggca ggataagatt cacataaacg cccagcgaaa
     9121 taagctcgcg ccagatgcgt aaaccttcct ctttcgaacc gattattact ggcactaccg
     9181 ggctaatatg tgaacttaac tcataaccca gtttttgcag accgtcatac agattgttgg
     9241 cgttatccat cagtttttgc cgcagctctg gttgtgtgga aatggttctt aacgacgagc
     9301 gcacggtcgc aatgcaggac ggagaaggag aggcggtaaa aatatacggg cggctgccgt
     9361 agcgcagcac ttccatcgct tctgaaccca cggcaaaacc accaatggaa gccaaacttt
     9421 tactgaaggt gccgacgata atatccacgt cgtcttcaac gccaacggct tcaaccagtc
     9481 cgcggcctgt tgcgcctaac acgccaaacg aatgtgcttc atcgacaatc aaataaccac
     9541 cgagacgacg tttgatatca acaatttctg ccagcggcgc aacgtcaccg agcatactgt
     9601 agatgccttc aacgataata atcgcctctt tcgcccgttc accgaggcgg accatgcgcc
     9661 gttccagatc gttgacgtca ttatggcgga aacgaataat ctctgcgccg ccgagagaac
     9721 aggcgtcata aatactggcg tggctgtcgg catccagtaa caccacggcg ttatggtcgg
     9781 caagtgcgct gatgacacct aaattagcgg tataactggt tgaaaaaacg atagcggtag
     9841 ggcgattgaa aaactcggca atctctttct ctaacgccag atgcggcgca taactgccgt
     9901 ttgccatacg agaaccggtg gtgccagtgc cttgggccgc taacgctgcc tgaccttcgc
     9961 ggatagcatc atgattaaag gttaaaccga gataattatt ggtcccggcc agaatcactt
    10021 ttttattgcc gatccggcct tcggtggcgg aatacacttc atcaatacag gtgccaaatg
    10081 gtgttaaccc gttgtcacta aattgcaggc gctcgccagc aaggcgcgca tatttatcgt
    10141 atagccccat tatttattct ccagccatgg aagtagagtt tccattaatt gcgcaggggt
    10201 tttgacatcc agcagaatat taataggaat ggaaatatcg aatctgtctt ccagcatcat
    10261 taagagatcc atcactttga tcgactccag gccaagatcg ttgacgagat cgctgtcggg
    10321 cttaatttcc acaccgtttt cgaccaaatc ctgtaaacag gaaaggatgt aatccattac
    10381 tatttcacga tttaccatag aaacgttaac acacctttaa acaagacaat caaaatagcc
    10441 cgttgcgtaa tgcgtgttcc aggctaatcc cgggaaacca attaatatct tcagaaatac
    10501 ggttattatt tgccgaccag tcggcgtggg ttaattcacg aattttcgag cgggtcagca
    10561 taggttcttt acctgccagg cgactcaacg cagtactgat atccgcaagg caggtgagta
    10621 gcggcagagg aatgccaacc atccgcacag aaccacaacg ggcttcggcg gcaagctgtc
    10681 gtacgcgttg ccagtcatag ccgccagcga cgccatcgca taattcatag gtttgcgtct
    10741 gtacagtttc ggcgtttaac cactgaccca ctgcttgcgc aaaatcggtg acgtgcagga
    10801 aagagagttg tgtatctggc gcaccaagtc gtggcagcag gccgcgcagc atccagtcaa
    10861 acagcggttt taactcttta tcgccgggac catacacggc tgtcgggcga aaaatgccca
    10921 gcgtaatttc gtcagccatt gcgatcagtc gttgttcggc gacgtgtttg gaatttgcgt
    10981 accaggagag ttcgggatgg cgcgccgcca gcgaagagat aaacagaaaa cgttggcaaa
    11041 agccgctctc ttttgccgct tgcatcaggc gcaggctgcc gtcaacgtta cagcgggtaa
    11101 aaatctcttc tttgtgtccg cgcacttgtc cggcgcaatg gaccaccgcg ctggctccgg
    11161 caaccagttc gctaagcgaa tgtgtatctt ccagcgaacc gcgcacccag atgagattat
    11221 cattgacgtg agcgcgggca gtacgcgtta atgcgcgaac gtgaaagccg cgggcgagca
    11281 ggttatcaat aatatattta ccgataaacc cggttgcgcc cgtcaccgcg acagtttgat
    11341 tcatgccagg gattcctgca catgaagact ggcagcataa gccttctgat aacgtttttt
    11401 cgcttccgca cgggcaggct tgccggagga tgttcgggga atactgtggg gcggcaacag
    11461 atcgatatcc gcggtaacgc caaattcgct ttggatccga gctgccagcg cgtggataag
    11521 ctgcccgcga cgttcttcgt cgctgatccg acactggatc tgcaaaatga ttttttcctg
    11581 ggcggtaaca aaagcaatcg catcgccaga atgaatttcc ggttcctgtt ccgcaatgta
    11641 ttcaatatcc tgcggccaga tattacggcc acgaataata atcagatctt taatgcgtcc
    11701 ggtgacatac agataaccgt ccagcagata accaaggtcg ccggtgtcta accagcccgt
    11761 cgcggcaatc tcgtcttgcg aaacctggtc gccaaagtac ccgctcatca ggctggggcc
    11821 ggagatgcaa atatggccta ccacacgttc cgcgacaggt atatctgctt cattgcggat
    11881 ttcaatgcca tgttccggca acgctttacc gcagttgacg aaagtcgata cggcgcgtgt
    11941 ttcagcaccc ggcgcgacgg ctttaccctg gtattcgagg atgtcgcgat ccacttcgtt
    12001 aaccacaacc ccgcaggctt catcagagaa gctgacagcc agcgcatttt ctgccagtcc
    12061 gtagcacggc atgaaagttt tatcgtcaaa gttaacctga cgaaaacatt cagcgaattg
    12121 atggagttgt tctgcggaga tcggttctgc tccaatacca gcgacgcgcc agcaggaaag
    12181 atccagttca gcgagatctt tttcattcac gcggcgctgg cacaattcat agccaaacgg
    12241 cggtgcaacg gaaacggtgc cgcgattttt actgatcaat ttaagccatt gcagaggacg
    12301 catggcaaaa tcctgagtgc gcaaataatc tactgaaagc tgcgtggcga cgggggtcag
    12361 gagaaagccg accagtccca tatcatggta gaaaggcagc caggagacgc agcggtcgcc
    12421 agggcgtaat ttaatgccat cgtggcttat agcacgtaga ttagccatca cttcgtgatg
    12481 ggtgataatg acgccacggg gaaaacgggt gctgccggag gtgtactgga ggtaggcgat
    12541 atcgtttgga actggacgct ggagcgcaac atcggcttcc ggtaatgcct taaaccaggc
    12601 gtggcttaaa acatgtaatt cggggttgtt gttatgcgtc gcggcattga ccagtggcaa
    12661 ccactcatca ccagtgataa tggctgcggg ctggcaactt gccagtaaac cctgcagttt
    12721 ggcgctccag gaatcccgct gaccgacgcc cattggaatc gccaacggga cggcgactaa
    12781 gccggcatac tggcaggcaa aaaaagcctc tacaaacccg ctactcgttt cggcaatcag
    12841 cgccacgcga tcgccttttt tcaggttcag cgataacaac cttttcgcac cagcttcgga
    12901 acgcgctttt aacgtctgat attccagttg atcttcaagt tggcaacgtc tgtcataaaa
    12961 attcattccg gcgctactca gagcggcgta gtccaaagca tcaaccagcg ttggaaaatc
    13021 ggcatagcgc ataggtaggg aatgcgtaaa gattttatta gacatataca ccacgaaaaa
    13081 attggatatc caaaataaaa tgacaataat gcggtggatt atataggcat aaaagataaa
    13141 tgagatagta ttttcatcgg tgtgaaaatg aacattgatt taaataatat gtttgagttt
    13201 ttttgttttt attgtgaatg atatttaatt tatgaatata ttaagaagga gccttgtccg
    13261 gtcgccttaa atcgacagga ggcattgtgg cgcttctgtg tagtctctga ccttatcgta
    13321 atcattaata aatactattt attctttgtt tgtattcatt gggttgatta ttaagatgcc
    13381 gttcgttgct gttgcaacct gtttttcacc gttaattatt tgttatccac agagatatgg
    13441 aatgcgatct gctgatgatt aaaattgaaa aagtaattaa taaaaatgac cttaaggcat
    13501 ttatcgcttt tccgtcatca ctttatcccg atgatccaaa ctggataccc cctttattca
    13561 ttgaacgcag cgagcatttg tctgcgaaga atccggggac ggaccatatc atctggcagg
    13621 cgtgggtggc aaaaaaagcg gggcagatag ttgggcgtat taccgcgcaa atcgatacct
    13681 tgcaccgtga gcgttacggt gaagataccg gtcattacgg aatgattgat gccattgatg
    13741 atccgcaggt ttttgccgcg ttgtttggcg cagcggaagc gtggttaaag tcacaaggtg
    13801 caaataagat cagcgggcct ttcagcctga atatcaatca ggaaagcgga ttactgattg
    13861 aaggttttga cactccgccc tgtgcgatga tgccacacgg caaaccgtgg tatgccgcgc
    13921 atattgaaca actgggttat cacaaaggca ttgatttact ggcgtggtgg atgcagcgaa
    13981 ccgatctcac gttctctccg gcgctaaaaa aactgatgga tcaggtgcgc aaaaaagtga
    14041 ccattcgctg catcaatcgc cagcggtttg ccgaagagat gcagatcctg cgtgagattt
    14101 tcaattctgg ctggcagcac aactggggat ttgtgccgtt taccgaacat gaattcgcga
    14161 ccatgggcga tcaacttaaa tatctggtgc cggacgatat gatctacatc gctgagattg
    14221 attctgcacc ctgcgcgttt attgttggct tgccgaacat caacgaggcg attgccgatc
    14281 tgaacggatc gctctttccc ttcggctggg caaaattgct gtggcgtttg aaagtcagcg
    14341 gtgtgcgaac tgcgcgagtg ccgctgatgg gcgtgcggga cgagtatcag ttcagccgca
    14401 tcggcccggt aattgcgctg ttattgattg aagccttacg cgatccgttt gcccgtcgga
    14461 agatcgatgc gctggagatg tcatggatcc tcgaaaccaa tacaggcatg aataacatgc
    14521 tggaacgcat tggggcggag ccgtacaagc gttaccgatt gtatgaaaag cagatttgat
    14581 ggcagtcacc cctgcttcat ctccagcggc accgccgagc gaatcaaatg gtggccttcc
    14641 agctcgatat cgtggagatt ctttttcacg gtccgcacat gatcacgtgc tgcgcgctgg
    14701 gcgacgtgcg gcagccgctg caacaccgcg ttgtagatcc gcgcatgctg gcgatcgatc
    14761 tgcttttttt gctgtggtcg atggtagaga ttatttacgc tggcaaacac tgaattaaac
    14821 atcagatcgg tcaatgattg cagcgtaaac accagcacct gattgtgaga agcctgacaa
    14881 atggcgagat ggaaagcgtg atccaactgc gcatgttcga tcagcgaaat ctctttgttg
    14941 ttctcactgg cggcgagcat tttttcataa cagcgggtta tcacaacaaa atcagcctgg
    15001 gttcccagcg ttgccgccag ccttgccgat tcgccctcca gtaatgcgcg aacgtcgagc
    15061 agatcgtaca gcgttcgcgg ctgcgtactg aacagatgga tcagcgggct ggcgtcctgc
    15121 acccgattaa gccgtgcgac acgagaatcg cgaccctgcg ccgtttcaat aatcccgcgc
    15181 ccgcgcagca cggtcagccc ttcacgcagt gcggagcgtg agaagccgag cttttcacac
    15241 agtcgacgtt ccgagggaag cggctgaccg accttcaaca cgccgtcgat aattaaccgt
    15301 tcgatactct ctgcaaccac ttcgcaaata gggcgacgtt catctttcat tcccggacct
    15361 cgtgcacagg taggaccaat ttttctgttc ttaactcgca aaacacgcac atcacgcaag
    15421 tgattatgtt acatcaattt aacattaagt taaccaaggc aaggtcacag agctggaaaa
    15481 aaaatggtct gaccggtagc taaagagata gacgaaaacg aaaagcccgc ttaataactg
    15541 ttcacagaag cagcgcgcaa aaatcagctg ccacacaaca caacaaagcg aagcctattc
    15601 atgagcatct tgtacgaaga gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg
    15661 gtactgatgg cactgcgtga gcatgtccct ggacttgaga tcctgcatac cgatgaggag
    15721 atcattcctt acgagtgtga cgggttgagc gcgtatcgca cgcgtccatt actggttgtt
    15781 ttgccaaagc agatggaaca ggtgacggcg attctggctg tctgccaccg cctgcgtgta
    15841 ccggtggtga cccgtggtgc aggcaccggg ctttctggtg gcgcgctgcc actggaaaaa
    15901 ggtgtgttgt tggtgatggc gcgctttaaa gagatcctcg acattaaccc cgttggtcgc
    15961 cgcgcgcgcg tgcagccggg cgtgcgtaat ctggcgatct cccaggccgt tgcgccgcat
    16021 aatctctatt acgcgccgga tccttcctca caaatcgcct gttcaattgg cggcaatgtg
    16081 gcggaaaatg ccggtggcgt tcactgcctg aaatatggtc tgaccgtaca taacctgctg
    16141 aaaattgaag tgcaaacgct ggacggcgag gcgctgacgc tgggatcgga tgcgctggat
    16201 tcgcctggtt ttgacctgct ggcgctgttc accggatcgg aaggtatgct cggcgtgacc
    16261 accgaagtga cggtaaaact gctgccgaag ccgcccgtgg cgcgggttct gttagccagc
    16321 tttgactcgg tagaaaaagc cggacttgcg gttggtgaca tcatcgccaa tggcattatc
    16381 cccggcgggc tggagatgat ggataacctg tcgatccgcg cagcggaaga ttttattcat
    16441 gccggttatc ccgtcgacgc cgaagcgatt ttgttatgcg agctggacgg cgtggagtct
    16501 gacgtacagg aagactgcga gcgggttaac gacatcttgt tgaaagcggg cgcgactgac
    16561 gtccgtctgg cacaggacga agcagagcgc gtacgtttct gggccggtcg caaaaatgcg
    16621 ttcccggcgg taggacgtat ctccccggat tactactgca tggatggcac catcccgcgc
    16681 cgcgccctgc ctggcgtact ggaaggcatt gcccgtttat cgcagcaata tgatttacgc
    16741 gttgccaacg tctttcatgc cggagacggc aacatgcacc cgttaatcct tttcgatgcc
    16801 aacgaacccg gtgaatttgc ccgcgcggaa gagctgggcg ggaagatcct cgaactctgc
    16861 gttgaagttg gcggcagcat cagtggggaa catggcatcg ggcgcgaaaa aatcaatcaa
    16921 atgtgcgccc agttcaacag cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt
    16981 gatcccgatg gtttgctgaa ccctgggaaa aacattccca cgctacaccg ctgtgctgaa
    17041 tttggtgcca tgcatgtgca tcacggtcat ttacctttcc ctgaactgga gcgtttctga
    17101 tgctacgcga gtgtgattac agtcaggcgc tgctggagca ggtgaatcag gcgattagcg
    17161 ataaaacgcc gctggtgatt cagggaagca atagcaaagc ctttttaggc cgccctgtca
    17221 ccgggcaaac gctggatgtg cgttgtcatc gaggcattgt taattacgac ccgaccgagc
    17281 tggtgataac cgcgcgtgcc ggaacgccgc tggtagccat tgaagcggcg ctggagagcg
    17341 ccgggcaaat gctcccctgc gagccgccgc attatggtga agaagccacc tggggcggga
    17401 tggtcgcctg cgggctggcg gggccgcgtc gcccgtggag cggttcggtc cgcgattttg
    17461 tcctcggcac gcgcatcatt accggcactg gaaaacatct gcgttttggt ggcgaagtga
    17521 tgaaaaacgt tgccggatac gatctctcac ggttaatggc cggaagctac ggttgtctgg
    17581 gtgtgctcac tgaaatctcg atgaaagtgt taccgcgacc acgcgccacc ctgagtctgc
    17641 gtcgggaaat cagcctgcaa gaagccatga atgaaatcgc ccagtggcaa cttcagccat
    17701 tacccattag tggcttatgt tacttcgaca atgcgttgtg gatccgcctt gagggcggcg
    17761 aaggatcggt aaaagcagcg cgtgaactgc tggggggcga agaggttgcg ggtcagttct
    17821 ggcagcaatt gcgtgaacaa caactgccgt tcttctcgtt accaggtacc ttatggcgca
    17881 tttcattacc cagcgatgcg ccgatgatgg atttacccgg cgagcaactg atcgactggg
    17941 gcggggcgtt acgctggctg aaatcgacag ccgatgacaa tcaaatccat cgcatcgccc
    18001 gcaacgctgg cggtcatgcg acccgcttta gtgccggaga tggtggcttt gccccgctac
    18061 cggctccttt attccgctat caccagcagc ttaaacagca gctcgaccct tgcggcgtgt
    18121 ttaaccccgg tcgcatgtac gcggaacttt gaggagcagg ctatgcaaac ccgattaaca
    18181 gaagagatgc ggcagaacgc gcgcgcgctg gaagccgaca gcatcctgcg cgcctgtgtt
    18241 cactgcggat tttgtaccgc aacctgccca acctatcagc ttctgggcga tgaactggac
    18301 gggccgcgcg ggcgcatcta tctgattaaa caggtactgg aaggcaacga agtcacgctt
    18361 aaaacacagg agcatctcga tcgctgtctc acttgccgca attgtgaaac cacctgtcct
    18421 tctggtgtgc gctatcacaa tttgctggat atcgggcgtg atattgtcga gcagaaagtg
    18481 aaacgcccac tgccggagcg aatgctgcgc gaaggattgc gccaggtagt gccgcgtccg
    18541 gcggtcttcc gtgcgctgac gcaggtaggg ctggtgctgc gaccgttttt accggaacag
    18601 gtcagagcaa aactgcctgc tgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat
    18661 aagcgtcggg ttttaatgct ggaaggctgc gcccagccta cgctttcgcc caacaccaac
    18721 gcggcaactg cgcgagtgct ggatcgtctg gggatcagcg tgacgccagc taacgaagca
    18781 ggctgttgtg gcgcggtgga ctatcatctt aatgcgcaag agaaagggct ggcgcgggcg
    18841 cgcaataata ttgatgcctg gtggcccgcg attgaagcag gtgtcgaggc aattttgcaa
    18901 accgccagcg gttgcggcgc gttcgttaaa gagtatgggc agatgctgaa aaacgatgcg
    18961 ttgtatgccg ataaagcgcg tctggtcagt gaactggcgg tcgatttagt cgaacttctg
    19021 cgcgaggaac cgctggaaaa actggcaatt cgcggcgata aaaaactggc cttccactgc
    19081 ccgtgtaccc tacaacatgc gcaaaagctg aacggcgaag tggaaaaagt gttacttcgt
    19141 cttggattta ccttaacgga cgttcccgac agccatctgt gctgcggttc agcgggaaca
    19201 tatgcgttaa cgcatcccga tctggcacgc cagctgcggg ataacaaaat gaatgcgctg
    19261 gaaagcggca aaccggaaat gatcgtcacc gccaacattg gttgccagac gcatctggcg
    19321 agcgccgatc gtacctctgt gcgtcactgg attgaaattg tagaacaagc ccttgaaaag
    19381 gaataacaaa atgaaaacta aagtcattct tagccagcaa atggcgagtg caattattgc
    19441 cgcaggtcag gaagaggcgc agaaaaataa ctggtctgtt tccattgctg ttgccgatga
    19501 cggcggtcat ctgctggcgt taagtcgcat ggacgattgc gcgccgattg cggcttacat
    19561 ctcccaggag aaagcgcgta ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga
    19621 gatggtgaac aacggacgta ccgcgttcgt gactgcgccg ttactgacgt cgctggaagg
    19681 tggcgtaccg gttgttgtgg atgggcaaat tattggtgcc gtgggcgttt ctggtttaac
    19741 cggagcacag gatgcgcagg tcgcgaaagc ggcagcagcg gtgttggcga aataagcgaa
    19801 aacgaggaga taaacaatga gtcaaaccat aacccagggc cgtttacgca ttaacgccaa
    19861 ttttaaacgt tttgtggatg aagaagtttt accgggaaca gggttggaca ctgcggcgtt
    19921 ctggcgcaat tttgatgaga tcgttcacga tctggcacca gaaaatcgtc agttgctggc
    19981 agaacgcgat cgcattcagg cggcgcttga tgagtggcat cgcagcaatc cggggccggt
    20041 aaaagataaa gcggcctata aatctttcct gcgtgaactg ggctacctgg tgccgcaacc
    20101 ggagcgcgtg acggtggaaa ccacgggcat tgacagcgaa atcaccagcc aggcggggcc
    20161 acagctggtg gttccggcaa tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg
    20221 gggctcactg tacgatgcgt tatacggcag cgacatcatc ccgcaggaag gggcgatggt
    20281 cagcggctac gatccgcaac gcggtgagca ggttatcgcc tgggttcggc gtttcctcga
    20341 tgaatcacta ccgctggaaa acggcagcta tcaggatgtg gtggcgttta aggtggtcga
    20401 taaacaatta cgcatccagt tgaaaaatgg taaagaaacc acgttacgta ccccggcgca
    20461 gtttgtcggt taccgtggcg atgccgctgc gccgtcctgc attttgctga aaaataacgg
    20521 cctgcatatt gagctacaaa tcgatgccaa cgggcggatt ggcaaagacg attcggcgca
    20581 catcaacgat gttatcgtcg aagcggccat cagtaccatt ctcgactgcg aagattcggt
    20641 tgcggcggtt gatgcggaag ataaaatcct gctgtaccgc aatctgctgg gtctgatgca
    20701 ggggactctg caagagaaaa tggagaaaaa cggtcggcaa atcgtgcgta aactgaatga
    20761 cgatcgtcag tacaccgctg ccgacggctc tgaaatttct ctgcacgggc gttcgctgct
    20821 gtttatccgc aacgtgggtc atttgatgac cattcctgtg atttgggaca gcgaaggcaa
    20881 tgaaatcccg gaaggcattc ttgatggcgt catgactggc gcgattgccc tctatgattt
    20941 aaaagtgcag aaaaactcgc gcactggcag cgtctatatt gtgaaaccga aaatgcacgg
    21001 tccgcatgaa gtggcgttcg ccaacaaact gtttacccgc attgagacaa tgctcggtat
    21061 ggcaccgaat accctgaaaa tgggcattat ggatgaagaa cgtcggacct cgctgaactt
    21121 gcgtagctgt atcgctcagg cgcgtaaccg cgtggcgttc atcaataccg gtttcctcga
    21181 ccgtaccggc gatgaaatgc attcggtgat ggaagctggc ccgatgctgc gtaaaaacca
    21241 gatgaaatcg acgccgtgga tcaaagccta cgaacgtaat aacgtgcttt ccggtctgtt
    21301 ctgtgggctg cgcggtaaag cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat
    21361 ggcagacatg tacagccaga agggcgacca gctgcgtgcc ggggcaaaca cggcctgggt
    21421 tccgtcgcca actgccgcta cgctccatgc gctgcactac caccaaacca acgtacagag
    21481 cgtgcaagcc aacattgccc agaccgagtt caatgctgaa tttgaaccgc tgctggacga
    21541 tctgctgact attccggttg ctgaaaacgc taattggtcg gcgcaagaga tccaacaaga
    21601 gctggataac aacgtgcagg ggattctggg gtacgtggtg cgttgggtgg agcaggggat
    21661 tggttgttca aaagtgccgg atattcacaa cgtggcgttg atggaagacc gcgcaacgct
    21721 gcgtatttcc agccagcata tcgccaactg gttacgtcac ggtattctga ccaaagatca
    21781 ggtgcaggcg tcgctggaga atatggcgaa agtggttgat cagcaaaacg ctggcgatcc
    21841 ggcttatcgt ccaatggtgg agaatttcgc taactcgtgt gcttttaaag ctgcctgcga
    21901 tttgatcttc ctcggagtga aacagccaaa cggctatacc gaaccgttat tacacgcatg
    21961 gcgtttacgc gaaaaagaaa atcattaata gcgtcaaaaa aagccccggc aagatttatt
    22021 gtcggggcta tgatagtaac tgcggtagta catttatccc ggaaataacc ggagcgatta
    22081 tcaggggtta acaatattaa atacccgtag cgcatcacta gcgggattaa aacaacaata
    22141 tggacatctt aacgatactc tgcgcctgcg tgtgcggaac agaaaccctt acccgatata
    22201 cttgttatgc cagaggtagc aattgcgtta gcggcgtact gttgctgtaa agtataacgc
    22261 ctatatttta cagttcagtc ctcttttgct tattacaacc ttaagtatat caagcatata
    22321 aagataataa gagactgaac aatatggtta cctggaccca aatgtatatg ccgatgggag
    22381 gactggggct atccgctctg gtcgccctga tcccaataat attcttcttc gttgcactcg
    22441 cggtattacg tctgaaagga catgtcgctg gagcaataac ccttatatta tctattctga
    22501 ttgcgatatt cgcctttaaa atgccgattg atatggcatt tgctgctgcg ggctatggct
    22561 ttatttatgg tttatggcct attgcgtgga ttattgtcgc ggcggtattc ctgtataaat
    22621 taaccgttgc cagcggccag ttcgatatta tccgcagctc ggttatctcc atcaccgacg
    22681 atcagcgttt gcaggtgtta ctgattggtt tctcctttgg tgcgctgctg gaaggggcgg
    22741 ctggctttgg cgcaccggtg gcgattaccg gtgctctact ggtgggcctg ggcttcaaac
    22801 cgctatacgc ggcggggctg tgtctgattg ccaacactgc gccggtggcg tttggtgcgt
    22861 tgggtgtgcc aattctggtc gccggtcagg taacgggaat cgatccgttc gacattggcg
    22921 caatggcggg acgtcagtta ccgttcctgt cggttcttgt gccgttctgg ctggtagcaa
    22981 tgatggacgg ctggaaaggg gtgaaagaga cgtggccagc ggcgctggtt gcagggggaa
    23041 gcttcgctgt cactcagttc tttacctcta actatattgg tccggaactg ccggatatta
    23101 cttcggcgct ggtgagtatc gtctcacttt ctttatttct taaagtctgg cggccaaaaa
    23161 ataccgaaac ggcaatcagt atgggacaat ccgcaggtgc gatggtggtg aataaaccgt
    23221 cttctggcgg tcccgtgcct tcagaatatc gtctggggca aatcattcgt gcgtggtcac
    23281 cgtttttaat cttaacggtg ttggtcacca tctggaccat gaagccgttt aaagcgttat
    23341 ttgctccggg cggcgcgttt tattcgctgg tgattaattt ccagatccct catttgcatc
    23401 aacaagtgtt gaaagcggcc cccattgtcg cccagccgac gccaatggac gcggtgttta
    23461 aattcgaccc cctctctgct ggtggcaccg ctatttttat tgcggcgatt atctcgatct
    23521 tcatcctcgg cgtggggatc aagaaaggta ttggtgtctt tgccgaaacg ctaattagtc
    23581 tgaagtggcc gatactgtcg attggcatgg tgctggcatt tgccttcgtg accaactatt
    23641 ccggcatgtc caccacgctg gcgctggtac tggctggtac aggcgtaatg ttcccgtttt
    23701 tctcaccgtt tctcggctgg ctgggcgtat tccttaccgg ctcggacacc tcctctaacg
    23761 ccctgtttgg ttcactgcaa tcgaccacgg cgcagcaaat caacgtctct gacaccctgc
    23821 tggtggcagc aaataccagc ggcggcgtaa ctggcaagat gatctccccg caatctatcg
    23881 ccgtggcctg cgccgcgacg ggcatggtgg gccgagaatc tgaactgttc cgctacaccg
    23941 tgaagcacag tctgattttt gccagcgtta tcggcattat cacgctgttg caggcgtatg
    24001 tgtttaccgg gatgttagtt tcgtaagtca ataatgccgg atgatcgtta atatcatccg
    24061 gctacattta cattattctg tcttattaat tgaaaattat ttccctcttt
//
LOCUS       DAZKJR010000076        24108 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKJR010000076 DAZKJR010000000
VERSION     DAZKJR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.076"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..2418
                     /gene="pdeF"
                     /locus_tag="ACHOWS_003010"
     CDS             175..2418
                     /gene="pdeF"
                     /locus_tag="ACHOWS_003010"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFQ0048554.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEISQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2457..3998)
                     /gene="ppx"
                     /locus_tag="ACHOWS_003011"
     CDS             complement(2457..3998)
                     /gene="ppx"
                     /locus_tag="ACHOWS_003011"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFQ0048555.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4003..6069)
                     /gene="ppk1"
                     /locus_tag="ACHOWS_003012"
     CDS             complement(4003..6069)
                     /gene="ppk1"
                     /locus_tag="ACHOWS_003012"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFQ0048556.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6240..6878)
                     /gene="purN"
                     /locus_tag="ACHOWS_003013"
     CDS             complement(6240..6878)
                     /gene="purN"
                     /locus_tag="ACHOWS_003013"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0048557.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6878..7915)
                     /gene="purM"
                     /locus_tag="ACHOWS_003014"
     CDS             complement(6878..7915)
                     /gene="purM"
                     /locus_tag="ACHOWS_003014"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0048558.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8240..8866
                     /gene="upp"
                     /locus_tag="ACHOWS_003015"
     CDS             8240..8866
                     /gene="upp"
                     /locus_tag="ACHOWS_003015"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0048559.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8952..10241
                     /gene="uraA"
                     /locus_tag="ACHOWS_003016"
     CDS             8952..10241
                     /gene="uraA"
                     /locus_tag="ACHOWS_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFQ0048560.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10336..11037
                     /gene="hda"
                     /locus_tag="ACHOWS_003017"
     CDS             10336..11037
                     /gene="hda"
                     /locus_tag="ACHOWS_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFQ0048561.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11143..11502)
                     /gene="arsC"
                     /locus_tag="ACHOWS_003018"
     CDS             complement(11143..11502)
                     /gene="arsC"
                     /locus_tag="ACHOWS_003018"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFQ0048562.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11523..12986)
                     /gene="bepA"
                     /locus_tag="ACHOWS_003019"
     CDS             complement(11523..12986)
                     /gene="bepA"
                     /locus_tag="ACHOWS_003019"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFQ0048563.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13199..14260
                     /gene="yfgO"
                     /locus_tag="ACHOWS_003020"
     CDS             13199..14260
                     /gene="yfgO"
                     /locus_tag="ACHOWS_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0048564.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14359..14829)
                     /gene="bcp"
                     /locus_tag="ACHOWS_003021"
     CDS             complement(14359..14829)
                     /gene="bcp"
                     /locus_tag="ACHOWS_003021"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFQ0048565.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14829..15401)
                     /gene="gcvR"
                     /locus_tag="ACHOWS_003022"
     CDS             complement(14829..15401)
                     /gene="gcvR"
                     /locus_tag="ACHOWS_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFQ0048566.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15547..16425
                     /gene="dapA"
                     /locus_tag="ACHOWS_003023"
     CDS             15547..16425
                     /gene="dapA"
                     /locus_tag="ACHOWS_003023"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0048567.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASMKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16442..17476
                     /gene="bamC"
                     /locus_tag="ACHOWS_003024"
     CDS             16442..17476
                     /gene="bamC"
                     /locus_tag="ACHOWS_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFQ0048568.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17689..18402
                     /gene="purC"
                     /locus_tag="ACHOWS_003025"
     CDS             17689..18402
                     /gene="purC"
                     /locus_tag="ACHOWS_003025"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0048569.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18570..19433
                     /gene="ypfJ"
                     /locus_tag="ACHOWS_003026"
     CDS             18570..19433
                     /gene="ypfJ"
                     /locus_tag="ACHOWS_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFQ0048570.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19448..21463
                     /gene="tmcA"
                     /locus_tag="ACHOWS_003027"
     CDS             19448..21463
                     /gene="tmcA"
                     /locus_tag="ACHOWS_003027"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFQ0048571.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATYVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDIVLSADDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            21537..22235
                     /gene="ypfH"
                     /locus_tag="ACHOWS_003028"
     CDS             21537..22235
                     /gene="ypfH"
                     /locus_tag="ACHOWS_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0048572.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     TATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22316..22516)
                     /gene="ypfN"
                     /locus_tag="ACHOWS_003029"
     CDS             complement(22316..22516)
                     /gene="ypfN"
                     /locus_tag="ACHOWS_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFQ0048573.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVNHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22544..23671)
                     /gene="dapE"
                     /locus_tag="ACHOWS_003030"
     CDS             complement(22544..23671)
                     /gene="dapE"
                     /locus_tag="ACHOWS_003030"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFQ0048574.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23675..24031)
                     /gene="yffB"
                     /locus_tag="ACHOWS_003031"
     CDS             complement(23675..24031)
                     /gene="yffB"
                     /locus_tag="ACHOWS_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFQ0048575.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5631 a         6015 c         6339 g         6123 t
ORIGIN      
        1 aacgtaataa ttcattaatc agggttattt tatttgatat atatcaattg gaactttcat
       61 atgacactta gaatcattgc tctctatagt gatgaataat catcattcga agtcaggtgg
      121 gatgtctgtc tgaatacacc tccttcagga tgtgggggat tctgctgagc atctatgaaa
      181 ctgaatgcaa cttatataaa aatacgtgat aaatggtggg ggcttccgct gttcctgcct
      241 tctttaatct tgcccatttt cgcccacatt aatactttcg cgcatatttc ttccggtgaa
      301 gtttttctct tttatctgcc actggcactg atgatcagca tgatgatgtt tttcagctgg
      361 gcggcattgc cagggatcgc cttagggatt tttgtccgca aatatgcaga gctgggtttt
      421 tacgaaacgc tctcattaac ggctaatttt attatcatta tcattctctg ttggggcggt
      481 tacagggttt ttactccccg gcgtaacaac gtttcacatg gtgatagccg tttaatttcc
      541 cagcgactat tctggcagat tgtgtttcct gcaacgctgt ttctgatact tttccagttt
      601 gctgcgtttg ttggattact ggcgagcaga gaaaatctgg tcggcgtcat gccctttaac
      661 ctcgggacct taatcaatta tcaggccttg ctggtgggta atctgattgg tgtcccgctg
      721 tgctacttca tcattcgggt ggtgcgaaat ccgttttatt tacgtagcta ttattcgcaa
      781 ttaaaacagc aggttgatgc caaagttacc aaaaaagagt tcgcaatctg gctactggca
      841 ttaggtgctt tactattgct gttatgcatg ccgttaaatg aaaaaagcac gatttttagc
      901 accaattaca ccttgtcatt attgctgccc ctgatgatgt ggggagcgat gcgctatggt
      961 tataagctga tttcattgct ctgggcggtc gtgttgatga tcagcatcca cagctatcaa
     1021 aattacattc ccatttatcc tggctatacc acgcagctga ccataacctc ctccagttat
     1081 ctggtattct cttttattgt caattatatg gctgtactgg caacccgtca gcgagcggta
     1141 gtcagacgca ttcagcggct tgcgtatgtg gacccggtgg ttcatctgcc aaatgttcgc
     1201 gccctgaatc gcgcgttacg tgatgccccc tggtctgcgc tttgttattt acgcatccct
     1261 ggcatggaaa tgctggttaa gaattatggc atcatgctgc ggattcaata caagcaaaaa
     1321 ctttctcact ggctgtcacc attgctggaa ccgggtgaag atgtttatca gctttcgggt
     1381 aacgatctcg cgctgcggct gaatacagaa tcgcaccagg agcgcattac cgcactggat
     1441 agccatctca agcaatttcg tttcttttgg gatggaatgc cgatgcaacc gcagattggc
     1501 gtcagttact gctatgtgcg ctcgccagtg aatcatatct acctgctgct gggagagcta
     1561 aatacagtgg ccgaactttc tatcgtgacc aacgccccgg aaaatatgca gcgtcgcgga
     1621 gcaatgtatt tgcaacgcga attgaaagat aaagtcgcga tgatgaatcg gctacagcgg
     1681 gcgctggaac acaaccattt tttcctgatg gcccagccga ttaccggtat gcgtggtgat
     1741 gtctaccatg aaattcttct gcgcatgaaa ggtgagaatg atgaactgat cagccccgat
     1801 agcttcttac cggtcgcgca cgaatttggt ttatcgtcga gtatcgacat gtgggtcatt
     1861 gagcatacgc tgcaatttat ggctgaaaac agagcgaaga tgcccgctca ccgttttgcg
     1921 attaatctgt cgccaacctc ggtatgtcag gctcgttttc ctgttgaaat cagtcagctg
     1981 ctggctaaat atcagattga agcgtggcaa cttatttttg aagtcaccga aagtaatgct
     2041 ctgaccaatg ttaagcaggc gcaaatcacc ttgcagcatc ttcaggaatt aggctgccag
     2101 attgcgattg atgatttcgg caccggctac gccagctatg cgcggcttaa aaatgtgaat
     2161 gccgatctgc ttaaaattga cggcagtttt atccgcaata ttgtgtcaaa tagtctggat
     2221 tatcagatag tggcgtcgat ttgccacctg gcgcgaatga agaaaatgcg ggtagtggca
     2281 gagtacgttg aaaacgaaga gatccgcgag gcggtgctct ctttggggat cgattatatg
     2341 cagggttatc ttattggtaa gccgcaaccg ttaattgata cgctgaatga aatcgaaccc
     2401 attcgcgaaa gtgcctgaat aatgcgggcc gacatttctc gtcggcccgc aaagcattaa
     2461 gcggcgattt ctggtgtact ttcttcttca attttcaacc gccagccagc cacgccttcc
     2521 cagtattctt gctccttttc cagatcaagc agtaccagcg cattctgact aaaccagtca
     2581 tgcggaaaac gcagtgtcca gtgactgtca tcggtaatca gcgtcaatgt tggcggtgtg
     2641 gtggttgcct gacgttgatt attgagtaat acgccaaggc gcaatagctg aatcagtggc
     2701 aggaactgtt tcttcttaaa caaggtaaag cgcggcagat cgtcgagctt aatcgcttta
     2761 cggtgatagc gcaccagcgt cgccatcatc aactgctgtt cctgattaaa acccggcaag
     2821 tcgctgtttt gcagaatata agcggagtgg cgatgcaaac cgctgtggtt gatattcaac
     2881 ccgacctcat gcagcatggc ggcccatcgc agtagcgctt ccagttgcgg atgcgccagc
     2941 ttcggttgct gttcccgcca ctgttcgtac atttgcatag tggtatccag cacccgtcgg
     3001 gcctgttcgc tgtcgatgtg atactggttg gcgaggctgc tggcggtgcg actacgcaca
     3061 tcctgatgac ggaaacgtcc ttccatttca tacagtacgc cttcgcgtaa cgccccgtca
     3121 gaaaggcgca gttcacggat ggctaaagca tcaaacacac cgcataaaat cgccagtcca
     3181 ggaacgaaga ctgttttccg ttcttcggaa agacctggta agctcagcga tgcgaaatta
     3241 cggtgccgta aaacttcttt taccagtttt tccagacgtt ccggggtaat tatcccgtct
     3301 ttctcgccca tttccattaa cacttcatgg gcggctttta ttgtaccgga agcgcccatt
     3361 gcaacattcc agccctgaat tcgaaattgc caggttaaag tttccagttt ttgtgctgcc
     3421 gccatgcgag cgcgctgaaa attctcttta ttgatgaccc cgccagggaa ataaagctgg
     3481 gcaaagctga cacaacccat ccggcggctt tcaacgagaa tcggttcgaa attttcacca
     3541 atcaccagtt ccgtagatcc gccgccaata tcaataacca gtttgcgacc tttctccggt
     3601 tgggtatgtt ccacgcccat aaaaatcaga cgggcttctt cattaccgga aataatttca
     3661 atcgggtagg gaatgacctt ttccgcgcgt ttcagaaagt cagtggcgtt cagcgcctga
     3721 cgcagtgtat gggtgccaac tatacagacg ctggcaggag aaaacccttg cagccgttcg
     3781 gcaaacagcg acagacagtt taaaccgcgc gtcattgcct cttcactcaa catattatct
     3841 ggccccaggc cgtccgccag atgcactcgc tgtttcaggc ggccaataat ctgcatggca
     3901 ccatctacca cacgggctat gaccatgtga aaactgtttg aaccaagatc gaccgcagca
     3961 aactcctgcg gacgagggga tttatcgtgt attggcatag ggttattcag gttgttcgag
     4021 tgatttgatg tagtcgtaaa tcgccaactg cgcccgtact ttacggcgat tgccgcgggg
     4081 aacgtagcga ttactgagtt ctttatcgat ataacgtgct ttgaccgtat cgctgaacaa
     4141 tatgtcgatg atgtccagca cccgctgctt caggcgcgga tcgagcagcg gtgtcgccac
     4201 ttcaatacga taatcaatat tgcgcgtcat ccagtcggcg gaagaaaggt agaccttttt
     4261 atcgccgcca ttttcaaaaa tataaacccg gtcatgttca aggtaacggt caacaatact
     4321 gatggcacga atgttgtcgc taatgccttc cagattgggg atcagcgaac acatgccgcg
     4381 aaccagcaga ttaaccggta cgccggagct ggaggccgca tacagacgat caaccaggcc
     4441 tttatcgaca aggttattta gcttcagggt gataccgctg ggcagccctt gctgcgcgtt
     4501 ggcgatttcg cggtccacca tttcatacaa caggcggcgg gagttttgcg gcgacaccat
     4561 taaataatca aatgtcaccg gacggtatgg gttttcaata aagttgaaca cccgccgtac
     4621 ttcgttggtt atgcgcgcat cggcggtcag caacgaatag tcagtataaa gacgcgcggt
     4681 tttttcgtta aagttcccgg tcccgatgtg tgcgtaacgc accacttcac cgttttcttt
     4741 acgtgaaatc aggaacagtt tggcgtgaat tttcagcccc ggcgcagaga agataacgtg
     4801 cacgcctgct tcggtcaggc gcttcgccca gtgaatgttg gcttcttcgt cgaaacgcgc
     4861 ctgtaactca accaccacgg tgactttctt accgttgtgt gcggcgtgga tcatcgagtc
     4921 gatgatgcgt gaatctttcg ccacgcggta aatgttaatt ttaatcgcca gtacgctcgg
     4981 gtcgaacgaa gcctgacgca gcagttccag tacatgctca aaggtatgat aaggataata
     5041 gagcaacaca tcgcgttcgc gaatagcatc aaaaccattg cggaactggg ctttatcaaa
     5101 ccaaatgtgg cgtaaacgcg gcagtggttt gttcaccaga ttggctttgc cgacattggg
     5161 gaaattaata aagtctttaa aattatgata acgaccaccg gggacgatgg agtcgtagcg
     5221 ggaaatagtc agtttttcgc gtaacacttc aaccagcgca ttgggcatat cgcgctgata
     5281 aacaaaacgc accggctcag cagttaaacg ctgcttgaga ctggaagaca tcaactccat
     5341 caggctggct tccatctcat gcactaaatc gtattcggca tcgcgggtca tcttcattga
     5401 ataggcattc agcgcgtcat aatcaaagaa gcctttgaaa atatcatcga ggcagtaacg
     5461 cagaatgtta tccagaagaa tcatcggctt gcgtcgacgt ggcgcttctg gcggtaaatt
     5521 cacaaagcgc ggcactttat ctgatgggat ctccagcagc gcgtaacgga tggtatcgcc
     5581 acggataatt tccaccgcca gataggtgta atcatctttc aggaactgca ctaagtcagt
     5641 gtcaggattg attaaaatcg gcgtaatgtg ctgacgcaga tactgcttaa aataatgacg
     5701 cagccagttt tgttgattga cggagagctg gcgttcatta atcaggaaga tctggttgcg
     5761 cgccatctcc agcaacagct cgttgtagag gccgtcgaat tcctgatcgg ctttcagcac
     5821 ccgggactga attttgccca gtaaatggcg ggaatgagag ttggaacctt gttcttcgct
     5881 aataatgatg cgtcgcttca gttcagcgaa gcggacttta tagaactcat caaggttatt
     5941 ggaatagatc cccaggaaac gcatcctttc aatcagcggg ttagatttgt ccgccgcttc
     6001 ctgaagcacg cgttcattga acgataacca actgagctct ttttcgatgt atagcttttc
     6061 ctgacccatt accactttta ctccgtttta ttcacgggac actgcctgga tattatggcg
     6121 agcaatattg gcagatgtcc aactatgcca gaaaaggggg aaaaaaactg ctggagcttg
     6181 cgatttacag gtgaattgaa aaacgccagc ggcagagctg gcgctttaac tacggggcat
     6241 tactcgtcgg cagcgtagcc ctgcggcggc agacgttgac catccagcca cgcggcgttt
     6301 tcgtgcattt tcagacgacc atcggcaaac cagctaatca ccagtggata aatagcgtgt
     6361 tcctgggttt gcacgcgggc ggtgatgtca tcttccgtat caccagcaaa taccgggact
     6421 ttcgcctgta aaataaccgg gccaccgtcc agttcatcgg tgacgaaatg caccgatgta
     6481 ccgtgctctt catcgccatt ttccagcgcc tgacgatggg tgtgtaatcc gggatatttc
     6541 ggcagcagag aagggtgaat gttcagcaaa cgcccggcat agtgggagac aaacgccggg
     6601 ctgagaatgc gcataaaacc agccagcacg accacatcgg gtgcgtacat gtcgatttca
     6661 tgaatcaact cccggtcata ggcttcacga ctgtcaaacg cgctggcgat gagcgtatgc
     6721 gttgcaatac ccgcctggcg ggcgcgttca aggccgaacg cgtcggcctt attgctgaaa
     6781 actgcccgta cggtgccttt aattttgttg gttttgcagg cgtcaataat tgcctgcaaa
     6841 ttacttccgt tgccggaaat aagcaccaca atattcatta ttcgataacc acgcgttgtt
     6901 cggaatcaga ggctttgatg ataccgattt tccacgcgtt ttcaccgttg gcattgagca
     6961 gggcgagagc tttgtccact tccggagcag gcagggcaat aatcataccg acgccgcagt
     7021 tgaaggtgcg atacatttca tggcgctcaa cattacctgc cgtttgcagc cagttgaaca
     7081 cttccggcca ctgccaggaa gattcatcaa tcactgcctg ggtattatct ggcaatacgc
     7141 gcggaatgtt ttcccagaag ccgccgccgg tcaggtgcgc aatggcatgc acatcgacct
     7201 tttcaatcaa tttcagcact gacttcacgt aaatgcgggt cggtgccagc agatgatcgg
     7261 ctaatggctt accatcaagt tcggtggttt gcggatcaca accgctgact tcaagaattt
     7321 tgcgcaccag cgaatagcca ttcgagtgcg ggccgctgga gccgagtgca atcagcacat
     7381 cgccgtcgct gactttagat ccgtcgatga tttctgattt ttctaccaca ccaacgcaga
     7441 aaccagcgac atcgtaatct tcaccgtgat acatccccgg catttccgcc gtttcgccac
     7501 ccaccagtga acagcccgat tgcagacaac cttccgcaat gccgctgatc accgctgaag
     7561 cggtatcaac atccagtttt ccggttgcgt aatagtcgag gaaaaacagc ggttccgcac
     7621 cttgcaccac caggtcatta acgcacatgg cgaccagatc aataccaatg gtgtcgtgac
     7681 gttttaagtc catcgccaga cgcagcttgg tacctacgcc gtcagtgccg gaaaccagca
     7741 caggttcacg atatttttgc ggcaatgcac acagcgcacc gaagccgccc agaccgccca
     7801 tcacttccgg acgacgcgtt ttcttcacta cgcctttgat tcttccaacc agagcattac
     7861 ccgcgtcaat atcaacaccg gcatctttgt agctaagaga ggttttatcg gtcactgctt
     7921 gggtccccac gcgttacttg cggtagaaaa ataaaattcg gcgcaattct aacagggaaa
     7981 gcaaacgttt gcgagactgc tttacacaac ctttttgcac gtcttttccc caggcgcgcg
     8041 gcgaaagaat acttgtacta gggcaaagga tcgttttcgg atggaataat cttcttccat
     8101 aaccatttga atataaaata actttatctc aaaccgttat cattttgact aaagtcaacg
     8161 aaaataatat tgccgccttg aagaaaggag gtataatccg tcgatttttt ttgtggctgc
     8221 ccctcaaagg agaaagagta tgaagatcgt ggaagtcaaa cacccactcg tcaaacacaa
     8281 gctgggactg atgcgtgagc aagatatcag caccaagcgc tttcgcgaac tcgcttccga
     8341 agtgggtagc ctgctgactt acgaagcgac cgccgacctc gaaacggaaa aagtcactat
     8401 cgaaggctgg aacggcccgg tagaaatcga ccagatcaaa ggtaagaaaa ttaccgttgt
     8461 gccaattctg cgtgcgggtc ttgggatgat ggacggtgtg ctggaaaacg ttccgagtgc
     8521 gcgcatcagt gtcgtcggta tgtaccgtaa tgaagaaacg ctggagccgg taccgtactt
     8581 ccagaaactg gtttctaaca tcgatgagcg tatggcgctg atcgttgacc caatgctggc
     8641 aaccggtggt tccgttatcg cgaccatcga cctgctgaaa aaagcgggct gcagcagcat
     8701 caaagttctg gtgctggtcg ctgcgccaga aggtatcgct gcgctggaaa aagcgcaccc
     8761 ggacgtcgaa ctgtatactg catcgattga ccagggactg aacgagcacg gatacattat
     8821 tccgggcctc ggcgatgccg gtgacaaaat ctttggtacg aaataaagaa taaaaataat
     8881 taaagccgac tttaagagtc ggcttttttt tgagtaaagc gcctataaca cataatacag
     8941 aggataatac tatgacgcgc cgtgctatcg gggtgagtga aagaccgcca cttttacaga
     9001 caatcccgct tagtttgcaa catttgttcg ccatgtttgg tgcaaccgtc ctggtgcccg
     9061 tcttatttca tattaacccg gcgactgtac tgttatttaa cggtatagga acactgctgt
     9121 atctcttcat ctgtaaaggg aaaattccgg cttatcttgg ttccagcttt gcctttattt
     9181 caccggtatt gttactgttg ccgttggggt atgaagttgc gctgggcggc tttattatgt
     9241 gcggcgtgct gttctgcctg gtttctttta tcgtgaagaa agcggggacc ggctggctgg
     9301 acgtgctgtt tccacctgcg gcaatgggcg caatcgttgc cgtcatcggt ctggagctgg
     9361 cgggcgtagc tgccggtatg gcgggtttac tcccggctga agggcaaacg ccagactcca
     9421 aaaccatcat catctctatt accaccctgg cggtcacggt tttaggttcc gtgctgtttc
     9481 gtggtttcct ggcaattatc ccgattttaa ttggcgtgct ggtggggtac gcgctctctt
     9541 tcgcaatggg aattgtcgat accacgccga ttattaatgc tcactggttt gcgctgccga
     9601 ccctctatac gccgcgcttc gagtggtttg ccattctgac tattctacca gcggcgttag
     9661 tggttattgc cgaacacgta gggcacctgg tcgtaacggc taatatcgtc aaaaaagatc
     9721 tgctgcgcga tccaggtctg caccgttcga tgtttgctaa cggcttgtcg accgtgatct
     9781 ccggcttctt tggctccacg ccaaacacca cttacggaga aaacattggc gtgatggcga
     9841 tcacccgtgt ttacagtacc tgggttatcg gcggtgcggc gattttcgct atcctgcttt
     9901 cctgcgtcgg taaactggct gcagctatcc agatgattcc attgccggtg atgggcggcg
     9961 tttcgctgct gctttacggt gtcatcggtg cttccggtat tcgtgtgttg atcgaatcga
    10021 aagtggatta caacaaagca cagaacctga tcctgacttc cgttattttg atcatcggcg
    10081 tcagtggcgc gaaggtaaac attggcgcgg cggagctgaa aggtatggcg ctggcgacca
    10141 tcgtcggtat cggcttaagt ttgatcttca aactgattag tatgctgcgc cctgaagaag
    10201 tggtgctgga cgcagaagac gcggatataa cagacaaata atgtaccgac cgggcagtgt
    10261 tgctgcccgg ttcaaacatc atgggattct gtggtaaact tctcgcgatt ttgtgaaatc
    10321 ctggttgagg tatctctgaa cacaccggca cagctctctt tgccacttta tcttcctgac
    10381 gacgaaacct ttgcaagttt ctggccgggg gataactcct ctttactggc cgcgctgcaa
    10441 aacgtgctgc gtcaggaaca tagcggttac atctatctct gggcacgcga aggcgcgggg
    10501 cgcagccatc tgctgcacgc ggcttgcgcg gaattgtcgc agcgtggcga tgcggtgggc
    10561 tatgtcccgc tggataaacg cacctggttt gttccggaag tgctcgacgg tatggagcat
    10621 ttgtcgctgg tctgtatcga caacattgag tgtattgcag gcgatgagtt gtgggagatg
    10681 gcgattttcg atctctacaa tcgaattctg gaatcgggca aaacacggtt gttaatcacc
    10741 ggcgatcgtc caccgcggca gttgaatctt ggattaccgg atctcgcgtc gcgtctcgac
    10801 tgggggcaga tctacaaatt gcagccactt tctgatgaag ataagttgca ggcgctacag
    10861 ttacgcgcgc gtttgcgtgg ttttgaactg ccggaagatg tggggcgttt cttgctgaag
    10921 cggctcgaca gagagatgcg cacgttattt atgacgttgg atcagttgga tcgtgcgtcg
    10981 attaccgcgc aacgtaagct gaccattccg tttgtgaaag aaattctgaa actgtagaca
    11041 agacgtttat tgtcggatat gcacccatag tattcgtcgg ccggataaga cgcggcaagc
    11101 gtcgcatccg gcaatgtgct tcaactagcg atggagcact tcttaaccca cgatttccag
    11161 tacctgctcc ggtggacgac caatccgcgc tttgccattc gcgaccacaa tcgggcgctc
    11221 cattagcttc ggattatcca ccattgcctg aatcagcgcc tcttcgctaa gcgaactgtc
    11281 agccaggttc agttctttat aaagatcctc tttctggcgc atcaattcac gagcgctatt
    11341 catccccagc attttcagca aatcacgcag cgttgccgca tcggcgggtg tctcaaggta
    11401 gagcaccact tctggctcca cgccgttttc tttcagcagg ttcagcgttt cccggctctt
    11461 tgaacagcgt gggttatggt agatttttac ctgtttggtc attgttcttc ctttaatgcg
    11521 aattacatct tggtataagg cttaaagcgt tcctgcagct ggcgcaactg gtcgatgcgc
    11581 gcatcgtaac gcgcttgttg caggctgcct aatttcacct gcgaactggc gctactcaac
    11641 agcgaaatgg cctgatcgag tcgtccggcg agcgcataac cttctgctcg cgcagccagc
    11701 tcctgatcgc ggttatttag cgcggcttcc gcctgtgcca gcaaatccca gccgttgctg
    11761 tcatctttat tattaaaggt gtagcgattc agaatattgg ccgcttcttg tggttgaccg
    11821 ccttgcagat aagcgttcgc caggttgagc tgcaacaccg gattggtgcg taaatcgcgg
    11881 gcatttttca gacgattgat cgcctcattg gctttgtttt gcccaagatc gatatcagta
    11941 gccagatcga gataccatgc gttgccaggt tctgccgcca gtaacggttg cagcgtttta
    12001 cgcgcctcgt cgtatttatt ggcttccatc gcctgtaaag cacgaccata ttgcgccgct
    12061 cgttgctgac gaacgtttcc tttcgcccat tcatccagca aatcactggt gagctggtta
    12121 cgtccggaat tatacatccc cagtgtgcgc gctttcgcca gatagaaatc ttccgacgac
    12181 tgcaccacca tcgggcgcat ctgattagca cggttgcggg catctgccag acgactttcc
    12241 ggcaacgggt gagtcaataa aatttccggc ggacgcgagg agtaacgcgc ctgatcgagt
    12301 aatttttcga ggaaggtcgg catcgcctgc ggatcgaatc ctgagcgttg cagcacctga
    12361 ataccaatgc ggtccgcttc ctgttcattt tgctgggtga agctgatcat cccctggcgc
    12421 gttcccgcca gtgtaccggt cagcgcagcc atccccgcct gcggactggc catcgccagt
    12481 aaaatagaac ctaacgcgcc gacccaggtc agcggcgcgc tgcgctgctg atcttccatc
    12541 gctcgcgcca ggtgacgttg ggtgacgtgg gagatttcgt gcgccataac tgaagccagt
    12601 tgactttcgt tatcggaata acggaacagg gcagagtgca gcaccacgtt gccgccaaag
    12661 aaagcaaagg cgttaatttc atcgttgttg atcagaaaga aatgaaacgg tgtcttaacc
    12721 gaattggcat gcgaaaccag acgcattcct agcgaattaa tgtattgcgt taacagcgga
    12781 tcattaatta acggcgcgct gccgcgtagc tggcggacat aatagtcgcc catctgcatt
    12841 tcctgaccaa tggaaagtgt gcttcctgcg gaggttccca tatccggcaa ggtgtctgcg
    12901 ctgtcggcaa acgccggggc tacctgacca atggtcatag cagcaatgag ggttgcaacc
    12961 aggttttttt tcaactgcct gaacataacc tctatcctgt atttctggag atagtcattt
    13021 gaccgatact gtgaaatatc gttcccaccg cgccgccttt gagtgtaacg gcgaagatta
    13081 gcccactcaa atgacttttc agtttcctga atccgtcata aaaagcgaag tcttttttgt
    13141 ggcatttcga tacaattgcg gatcgcaatc ccgctcttga ggttcaggga aggtctgtat
    13201 gctcgaaatg ttgatgcaat ggtatcgccg ccgttttagc gacccggaag cgattgcctt
    13261 gctggttatt ttagttgccg gatttggcat tatctttttc tttagtggcc tgcttgctcc
    13321 gctgttggtg gctattgtgc tggcctattt gctggaatgg ccaaccgtgc gcctgcaatc
    13381 tattggctgc tcccgccgct gggcgacgtc gattgtattg gtggttttcg tcggtatatt
    13441 gctactgatg gcgttcgtgg tactgcctat cgcctggcag cagggcatct acttaatccg
    13501 cgatatgccg gggatgctca ataagctttc tgactttgcc gccacgttgc cgcgccgcta
    13561 tccggcgtta atggatgcgg gcattattga tgcaatggcc gaaaatatgc gcagtcggat
    13621 gctgaccatg ggcgattcgg tggtgaaaat ttccctcgcc tcgctggtcg gtttgctgac
    13681 cattgccgtc tatctggtgc tggtaccatt gatggtcttc ttcctgctga aagacaaaga
    13741 gcagatgctg aacgccgttc gccgggtgct gccgcgtaac cgtggactgg caggacaggt
    13801 gtggaaggag atgaatcaac aaatcaccaa ctatatccgc ggcaaagtgc tggagatgat
    13861 cgtggtgggg attgccacct ggctgggatt cttactcttc gggctgaact attcgctgct
    13921 gctggcggtg ctggtcggct tctcggtact tattccgtac attggtgcat ttgtggtgac
    13981 cattccggtg gttggcgtgg cgctattcca gtttggtgct ggcacggaat tctggagctg
    14041 cttcgcggtg tatctgatca ttcaggcgct ggacggcaac ctgttagtac ctgtgttgtt
    14101 ctccgaagcg gttaacctgc atccgctggt cattatttta tcagtagtga tcttcggtgg
    14161 tttgtgggga ttctggggcg tattcttcgc cattccattg gcgacgctga tcaaagccgt
    14221 gattcacgcc tggcccgatg ggcaaatcgc gcaagactga ccttcagcga aaaaatttga
    14281 aacgagtgtt gcagggcagt gagaagatgt gagatgtgct ggccgcaagc gcagccagca
    14341 cggaatggag caaagtaatc aggcgtgttc tttcagccag ttcagcacaa cgtcgtggtg
    14401 attgctggtt ttgaaatcgt caaagacatg ttcgattttg ccatcagcgt caatcaggaa
    14461 gctgatgcga tgaataccat cgtaggtttt gcccatgaag gacttttcac cccagacgcc
    14521 gaattgttcg cacacctggt ggtcctcatc agacaggagc gtaaagttaa gcagctcttt
    14581 ttccgcaaaa cgggagagtt tttcgggttt atcggtgctg atacccagca catcaacgcc
    14641 cgcttttttc aactcatcca tgttatcgcg taagccgcag gcctgtacgg tacagccggg
    14701 ggtcatggct ttcgggtaga aataaaccag aacacgctgt ccctggaagt cggtcaaatt
    14761 aacttgttct ccgtcttgat ccggcaagct aaatttcggt gcgatatcac cggctttcag
    14821 tggattcatt acttaactcc atcctgttca tcatgttggg aataattgac gacgttaata
    14881 ctgccttgtg cattgagttc tgtacatagg gctttgaatg cttgctcaat atttgctgcg
    14941 tccgcagatg cggggctgtg ggcggttatc tgaatatgca actgcgcagc ccgttcattt
    15001 tcagcaggtt gcgtgcgcga caccagctcc gcaatgttca tatgatgcgc gtcgaaaagt
    15061 gctgtgaagc gttcaattaa atgcggggag tctgccacat cgacctgaac ccagacagat
    15121 gctggcattg gcggacgcgg acgcgccgtc gtgcgcttca tcacgattaa aagatccagc
    15181 tcggcacctt tcaacggtaa cgttgattca atcagagtaa tggcattcca tgaaccggaa
    15241 agcagcatga taaacgtgaa ctcttctccc agcatcgcca ggcgactgtc ttcaatatta
    15301 cagccgcaac tactgacatg acgggtgatg gtgttcacaa ttccagggcg atcggcaccc
    15361 aacgcagtga tcaccagata atgttgcgat gacagtgtca aactggttat tccttcaagg
    15421 ggtgagttgt tcttaaggaa agcataaaaa aaacatgcat acaacaatca gaacggttct
    15481 gtctgcttgc ttttaatgcc ataccaaacg taccattgag acacttgttt gcacagagga
    15541 tggcccatgt tcacgggaag tattgtcgcg attgttactc cgatggatga aaaaggtaat
    15601 gtctgtcggg ctagcatgaa aaaactgatt gattatcatg tcgccagcgg tacttcggcg
    15661 atcgtttctg ttggcaccac tggcgagtcc gctaccttaa atcatgacga acatgctgat
    15721 gtggtgatga tgacgctgga gctggctgac gggcgcattc cggtgattgc cgggaccggt
    15781 gctaacgcta ctgcggaagc cattagcctg acgcagcgct tcaatgacag cggtatcgtc
    15841 ggctgcctga cggtaacccc ttactacaat cgtccgtcgc aagaaggttt gtatcagcat
    15901 ttcaaagcca tcgctgaaca tactgacctg ccgcaaattc tgtataatgt gccgtcccgt
    15961 actggctgcg atctgctccc ggaaacggtg ggccgtctgg cgaaagtaaa aaatattatc
    16021 ggaatcaaag aggcaacagg gaacttaacg cgtgtaaacc agatcaaaga gctggtttca
    16081 gatgattttg ttctgctgag cggcgatgat gcgagcgcgc tggacttcat gcaattaggc
    16141 ggtcatgggg ttatttccgt tacggctaac gtcgcagcgc gtgatatggc ccagatgtgc
    16201 aaactggcag cagaagggca ttttgccgag gcacgcgtta ttaatcagcg tctgatgcca
    16261 ttacacaaca aactatttgt cgaacccaat ccaatcccgg tgaaatgggc atgtaaggaa
    16321 ctgggtcttg tggcgaccga tacgctgcgc ctgccaatga caccaatcac cgacagtggt
    16381 cgtgagacgg tcagagcggc gcttaagcat gccggtttgc tgtaaagttt agggagattt
    16441 gatggcttac tctgttcaaa agtcgcgcct ggcaaaggtt gcgggtgttt cgcttgtttt
    16501 attactcgct gcctgtagtt ctgactcacg ctataagcgt caggtcagtg gtgatgaagc
    16561 ctacctggaa gcggcaccgc ttgcggagct tcatgccccg gctggaatga ttttgccggt
    16621 gacctccggc gattatgcaa tcccggtgac caacggtagt ggtgctgtcg gtaaggcgct
    16681 ggacattcgt ccaccagccc agccgttggc actggtttct ggcgcgcgta cccagttcac
    16741 gggcgatacc gcttcattgc tggtggaaaa tggtcgtggc aatactctgt ggccgcaggt
    16801 ggttagcgtg ctgcaggcga aaaactacac catcacccaa cgtgatgatg ctggtcagac
    16861 actgaccacc gattgggtac aatggaaccg tctggacgaa gacgagcagt atcgtggtcg
    16921 ttatcaaatc tctgttaagc cgcagggtta tcagcaggcg gttacggtta aactgctgaa
    16981 cctggaacag gcgggcaaac cggttgcaga cgcggcttcc atgcagcgtt acagcacgga
    17041 gatgatgaac gttatttccg ccggtctgga taaatctgcc actgacgccg cgaacgctgc
    17101 gcaaaatcgt gcctccacca ctatggacgt acaaagtgca gctgatgaca ccggtttacc
    17161 aatgctggtc gtacgcgggc cgttcaatgt ggtttggcaa cgtctgccag ctgcgctgga
    17221 aaaagtgggc atgaaagtga ccgacagcac ccgttcgcag ggcaacatgg ccgtaactta
    17281 caagccgctg tctgacagcg aatggcacga actgggcgcg agcgatccag gtctggcatc
    17341 cggtgactat aaactgcagg ttggcgattt agataaccgc agcagcctac agttcatcga
    17401 tccgaaaggt catactctga ctcagagtca gaacgacgcg ctggtagctg tcttccaggc
    17461 tgcgtttagc aagtaaaaat acagggctgg agtcatccgg cccttttttc tgatatgata
    17521 cgcaaacgtg tgcgtcggca gaaaaacgcg attttagcgg taattcgcac gaaatttgtt
    17581 tgtcggacgt aggtcggata aggcgttcac gccgcatccg acaaaacatc cggcacacca
    17641 gacagcaata gattttaaaa cgttaattca cacccaggag tgataaagat gcaaaagcaa
    17701 gctgagttgt atcgtggtaa agcgaaaacc gtatacagca cggaaaaccc ggacctgttg
    17761 gtgctcgaat tccgcaatga tacgtcagca ggggatggcg cgcgcattga gcagtttgat
    17821 cgcaaaggta tggtgaacaa caagttcaac tacttcatta tgagcaaact ggctgaagcg
    17881 ggtatcccga ctcaaatgga gcgtctgctc tccgataccg aatgtctggt gaaaaagctg
    17941 gatatggtgc cggttgagtg tgtcgtgcgt aaccgtgctg ctggctctct ggtgaaacgt
    18001 cttggaatcg aagaaggtat tgagctgaac ccgccactgt tcgatctgtt cctgaaaaac
    18061 gacgccatgc acgatccgat ggtcaacgaa tcttactgcg aaacctttgg ctgggtaagc
    18121 aaagagaacc tggcacgcat gaaagagctg acctacaaag cgaacgacgt gctgaaaaaa
    18181 ctgttcgatg atgctggtct cattctggtc gacttcaagc tggagtttgg tctgtacaaa
    18241 ggtgaagtgg tactgggcga tgagttctcc ccggacggca gccgcttgtg ggacaaagaa
    18301 acgctggaga aaatggacaa agaccgtttc cgccagagcc tcggtggcct gatcgaggcc
    18361 tatgaagccg tcgcccgccg cctgggtgta cagctggact gatttttctg ttcatcatct
    18421 tgccgtgcag ctggcacggc aagacaaccg ctctcataag atgagtattg aacgctcatt
    18481 cctccgccat ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc tacgatcatt
    18541 tctataatga atatattgtt gaggtatcta tgcgttggca agggcgacgt gaaagtgaca
    18601 atgttgaaga caggcgcaac agctctggtg gtccgtcaat gggcggtcca ggttttcgcc
    18661 tgccaagcgg taaaggcggg ctgattttac tgatagtcgt gctggttgca ggctactatg
    18721 gcgttgattt aaccgggttg atgaccggac agccggtttc ccaacagcaa tcaacacggt
    18781 caattagtcc taatgaagac gaagccgcaa aattcacttc ggtgattctg gcaaccacgg
    18841 aagacacctg gggacagcaa ttcgagaaga tgggtaaaac ctatcagcaa ccgaagctgg
    18901 tcatgtaccg tggaatgacg cgcaccggct gcggggcggg ccagtccata atgggaccgt
    18961 tctattgccc ggcggatggc acggtttata tcgatctctc cttctatgat gacatgaaag
    19021 acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgcccat gaagtcggtc
    19081 atcatgtgca gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga
    19141 cgcaggcgga agtgaaccgc ttatctgtgc gtatggaact ccaggccgac tgttttgccg
    19201 gtgtctgggg gcatagtatg cagcagcaag gtgttctgga aaccggcgat ctggaagagg
    19261 cgctgaacgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag
    19321 tagtaccaga cagtttcact catggcactt ctcagcaacg ctacagctgg tttaaacgtg
    19381 gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt taacttccgg
    19441 ggcagggatg gctgaactga ctgcgcttca cacattaaca gcgcaaatga aacgtgaagg
    19501 gatccgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc atgcgcttaa
    19561 gttgcgtgat gccttacctg gcgactggct gtggatttcg ccgcagccag atgctgaaaa
    19621 ccactgttct ccctcggcgc tacaaacttt acttgggcgc gagttccggc atgcggtatt
    19681 cgacgcccgc cacggctttg atgccgctgc ctttgcggca cttagcggaa cgttgaaagc
    19741 gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc aacctgatgc
    19801 cgactcgctg cgatggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca
    19861 tctcaaacgc gtacttacgg cgaataacga cgctatcctc tggcggcaga accagccgtt
    19921 cacgttggcg cattttactc cccgtactga ctggcacccc gcgaccggcg caccacagcc
    19981 agaacaacag caactcttac agcagctact gaccatgcca ttgggcgtgg cggtggtaac
    20041 ggctgcgcgt gggcgcggta aatcggcgct ggcagggcaa ctcatttctc gtattgcggg
    20101 tagtgcgatt gtcaccgcgc ccgcaaaagc ggcaacgtat gtactggcac aatttgcggg
    20161 cgagaagttt cgctttattg cgccggatgc cttgttagcc agcgatgagc aagccgactg
    20221 gctggtggtc gatgaagccg cagccatacc tgcgccgttg ttgcatcaac tggtatcgcg
    20281 ttttcctcga acgttgttaa ccactacggt gcagggctac gaaggcacgg gacgtggttt
    20341 tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactgc aacagccgat
    20401 ccgctgggca cagggatgcc cgctggaaaa aatggttagt gaggcactgg tttttgacga
    20461 tgaaaacttc acccacacac cacaaggcaa tatcgtcatt tccgcatttg aacagacgtt
    20521 atggcgaagc gagccacaaa cgccgttaaa ggtttatcaa ttattgtctg gtgcgcacta
    20581 ccggacttcg ccgctggatt tacgccgcat gatggatgca ccagggcaac attttttaca
    20641 ggcggctggc gaaaacgcga ttgccggggc gctgtggctg gtggatgagg gcggattatc
    20701 tcaagaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta atctggtggc
    20761 ccagtcgctg gcggcgcacg gcagcaatcc actggcggcg acattgcgcg gaaggcgggt
    20821 tagccggata gcagttcatc cggctcgtca gcgggaaggc acaggtcagc aactgattgc
    20881 tggtgctttg caatatacgc atgacctcga ctatctttcg gtgagttttg gttacaccgg
    20941 ggaattatgg cgtttctggc aacgctgcgg ttttgtgctg gtgcgaatgg gtaatcatcg
    21001 tgaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagtg atgcgggtaa
    21061 acagctggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcaaagtg
    21121 gaatggcgaa acgctccctg ttgatccact aaacgatatc gtgctttctg ccgacgactg
    21181 gcttgaactg gccggttttg ctttcgctca tcgtccgcta ttaacgtcgt taggttgctt
    21241 aatgcgtctg ttacaaacca gcgaaatggc attaccggcg ctgcgtgggc gtttacagaa
    21301 aaacgccagc gacgcgcagt tatgtaccac acttaaactt tcaggccgta agctgttact
    21361 ggtccgtcag cgggaagagg ccgcgcaggc gctatacgca cttgatgatg ttcgcactga
    21421 gcgtttgcgc gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc
    21481 catggtcatt gtgagtcagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga
    21541 aacatgacca ttttgttgtt caaagcccgg ataaacctgc gcaacagttg ttactgcttt
    21601 tccacggtgt cggggataac ccggtggcaa tgggggaaat aggtagctgg tttgcgccgc
    21661 tatttcctga tgcgctggtg gtgagtgtcg gaggcgcgga gccgagcggt aatccggcgg
    21721 ggcgtcagtg gttttcggtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg
    21781 ccatcatgcc gacgtttatt gagacggtgc gctactggca gaaacagagc ggagtagggg
    21841 ctaccgccac ggcgttaatc ggtttctctc aaggcgcgat tatggcgctg gagagcatta
    21901 aagccgaacc gggccttgcc tcacgcgtca ttgcctttaa cggacgctat gcgagcctgc
    21961 cggaaactgc gtctacagcc acgacaattc acctgattca tggcggtgaa gatccggtta
    22021 tcgatctggc gcatgcggtt gcggcgcagg aagcgttaat cagtgccggt ggtgacgtga
    22081 cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caatttgctc
    22141 tggatcactt gcgttatacc attccgaaac attatttcga cgaagcgcta agcggcggta
    22201 agcctggcga tgatgatgtg attgaaatga tgtaataata gccgggtagg tctgataaga
    22261 ctgtatcagg catcatggcc ggatgcggcg ttaacgcctt atccggccac ttagcttact
    22321 tcttcggttg atcctttttc ggccagtcgt cgtcatcgtc ccacttatcg ttgaaatcac
    22381 gatgcggggg aagctccggc ttgttggcga gaaatttctt atggttgacg cgcttgagat
    22441 ctttaatcac gttcagcagg acgcctacca aaaagacaat caccagaatc caccaatatt
    22501 tagccagcca gtccatgctt atttcctctt gcagaaccac tcatcaggcg acgagctgtt
    22561 ccatgatacg ttgatacata cgggcaagta gctgcaggtc ggcagcgttc acacattcat
    22621 taattttatg aatagtggca ttgaccggcc cgagttccac cacctgcgcc cccatgcggg
    22681 caataaagcg cccgtcggac gttccgcctg tggttagtag ctgcggttta atttcattat
    22741 agtgctcaac cgcgttaacg accgcatcca ccagtttacc gcgcgcggtc aaaaatggct
    22801 gcccggaaag ccaccaatcc accgtatagc gcagttgatg tttttcaagc agggcaagta
    22861 cctgcgcttt gatcatctca tcagtcagtt cggtgctgaa gcggaagtta aactgcacaa
    22921 acagttcacc cggaataacg ttgttactgc ccgttcccgc ctgaatattg gcaatctgca
    22981 tactggtcgc cgggaagaat tcattgccct gatcccactc aatagccact aattcattaa
    23041 ggaaaggtgc tgcgcgatgt accggattgt cagccagatg tgggtaggca acatgcccct
    23101 gcacgccatg aatggtgagg ttgcaggtta acgatccgcg acgaccattt ttcaccacat
    23161 caccaaccac ttcgatactc gacggttcgc caaccagaca gtaatcgaga cgctcattac
    23221 gcgccattaa cgcttcgacg acttttaccg taccgttgtg ggcactagct tcttcatcag
    23281 aggtgatcag aaatgccagt cgccccgtat ggttgggatg ttgtgcgaca aaacgttctg
    23341 ccgctaccac catcgccgcc agcgagcctt tcatatctgc cgcaccgcgc ccgaataaca
    23401 tgccgtcacg aatggtgggt tcaaacggcg gattgatcca acggtcggcg tcgccaggcg
    23461 gcaccacgtc ggtatgcccg gcaaaggcta acgtttcacc ctgcccacgc catgcccaaa
    23521 aattctgcgt atcggcaaag tccatgcgtt caacggtaaa accgatcgcc tgcaaacgtt
    23581 caatcaacaa agcctggcat cctgcgtcat caggactcag ggaagggcgg cgaataagct
    23641 gttgtgtcag ctcaataacc gggcacgaca tagactacac ctcatggaaa aattgctgat
    23701 aactggattc actgaaaccc agcagcatag gcttaccagg cgcgcagagc aatggacgtt
    23761 tgataattgc aggcatttca gtcattaatg tcgccgcaga ggccgcatcg gtgattttat
    23821 tgcgggtggt ttcgtccagt ttacgccagg ttgtaccacg ggtgttgagt aacgcttccc
    23881 agcctaattc gttgataaaa ccgttcaata attcgctgtc caatccatcg acgcggtaat
    23941 catgaaagcg atagtcgatg ttattggctt ccagccaacg gcgagccttt ttaatggtgt
    24001 cacaattttt gatgccgtaa agtgtaacca taatgaatcc tttttcgatt tatgagaata
    24061 aatactcatt taagggtgaa aacattttgc ctgcaaatga tcatcagg
//
LOCUS       DAZKJR010000077        23970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKJR010000077 DAZKJR010000000
VERSION     DAZKJR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.077"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(249..1898)
                     /gene="pgi"
                     /locus_tag="ACHOWS_003032"
     CDS             complement(249..1898)
                     /gene="pgi"
                     /locus_tag="ACHOWS_003032"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0048576.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2135..2331
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2423..3772
                     /gene="lysC"
                     /locus_tag="ACHOWS_003033"
     CDS             2423..3772
                     /gene="lysC"
                     /locus_tag="ACHOWS_003033"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFQ0048577.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4025..4297
                     /locus_tag="ACHOWS_003034"
     CDS             4025..4297
                     /locus_tag="ACHOWS_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFQ0048578.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4298..5170)
                     /gene="rluF"
                     /locus_tag="ACHOWS_003035"
     CDS             complement(4298..5170)
                     /gene="rluF"
                     /locus_tag="ACHOWS_003035"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFQ0048579.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5518..6465
                     /locus_tag="ACHOWS_003036"
     CDS             5518..6465
                     /locus_tag="ACHOWS_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFQ0048580.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVNALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6535..7341
                     /locus_tag="ACHOWS_003037"
     CDS             6535..7341
                     /locus_tag="ACHOWS_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0048581.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTRLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            7351..7758
                     /locus_tag="ACHOWS_003038"
     CDS             7351..7758
                     /locus_tag="ACHOWS_003038"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFQ0048582.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     HSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7758..8252
                     /locus_tag="ACHOWS_003039"
     CDS             7758..8252
                     /locus_tag="ACHOWS_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0048583.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQHIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8318..9115
                     /locus_tag="ACHOWS_003040"
     CDS             8318..9115
                     /locus_tag="ACHOWS_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0048584.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9126..9950
                     /locus_tag="ACHOWS_003041"
     CDS             9126..9950
                     /locus_tag="ACHOWS_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0048585.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLLVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10002..11231
                     /gene="sorE"
                     /locus_tag="ACHOWS_003042"
     CDS             10002..11231
                     /gene="sorE"
                     /locus_tag="ACHOWS_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFQ0048586.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11613..12302
                     /gene="pepE"
                     /locus_tag="ACHOWS_003043"
     CDS             11613..12302
                     /gene="pepE"
                     /locus_tag="ACHOWS_003043"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFQ0048587.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12392..14023)
                     /locus_tag="ACHOWS_003044"
     CDS             complement(12392..14023)
                     /locus_tag="ACHOWS_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFQ0048588.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14243..17926)
                     /gene="metH"
                     /locus_tag="ACHOWS_003045"
     CDS             complement(14243..17926)
                     /gene="metH"
                     /locus_tag="ACHOWS_003045"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0048589.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMEFLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLSGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18126..18950
                     /gene="iclR"
                     /locus_tag="ACHOWS_003046"
     CDS             18126..18950
                     /gene="iclR"
                     /locus_tag="ACHOWS_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFQ0048590.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(18967..20691)
                     /gene="aceK"
                     /locus_tag="ACHOWS_003047"
     CDS             complement(18967..20691)
                     /gene="aceK"
                     /locus_tag="ACHOWS_003047"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFQ0048591.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGNLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRK
                     QRFSVRFV"
     gene            complement(20907..22211)
                     /gene="aceA"
                     /locus_tag="ACHOWS_003048"
     CDS             complement(20907..22211)
                     /gene="aceA"
                     /locus_tag="ACHOWS_003048"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFQ0048592.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(22241..23842)
                     /gene="aceB"
                     /locus_tag="ACHOWS_003049"
     CDS             complement(22241..23842)
                     /gene="aceB"
                     /locus_tag="ACHOWS_003049"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFQ0048593.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         5620 a         6325 c         6262 g         5763 t
ORIGIN      
        1 ttaggattaa cctcacggta tgatttccgt taaatcacag acaaggggat ttatctgata
       61 aaaaatgccg acataacgtc ggcattgttt ttaaggctat ttaatcaatc gtactatgta
      121 ttaattgccg aatacgatgt acatatcggc atcgacctgt aggcctgata agacgcgacc
      181 gcgtcgcatc aggcatcggt tgccggatgc ggcgtgaacg ccttatccgg cctacatatc
      241 gacaatgatt aaccgcgcca cgctttatag cggttaatca gaccattggt cgagctatcg
      301 tggctgctga tttctttatc atctttcagc tctggcagaa tacggttcgc cagctgttta
      361 cccagctcca cgccccactg gtcgaaggtg aagatgttca gaatcacgcc ctgagtaaag
      421 attttgtgct catacagcgc aatcaatgca cccaggctga acggagtgat ttcacgcagc
      481 aggatggagt tggtcgggcg gttaccttcg aacactttga acggcaccac gtagtcaagc
      541 gttgccggat ctttaccctg atcacgatat tcctgctcaa ccacttcgcg ggatttacca
      601 aacgccagcg cttcggtctg agcaaagaag ttagacagca gtttctggtg atgatcagag
      661 agcgggttat gggtgatagc cggagcgatg aaatcgcacg gtaccatttt ggttccctgg
      721 tggatcagct gatagaacgc gtgctgaccg ttcgtgccag gttcacccca gataatcggg
      781 ccagtctggt aatccacaac cttaccgtta cggtcaacat acttaccgtt ggattccata
      841 ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact ggtcatacgg cagaatcgct
      901 tcagtttccg cgccaaagaa attgttgtac cagatgccaa tcagcgccag caagacaggc
      961 aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca tcgcgtgtgc gccagaaagc
     1021 agttcaacaa aattatcaaa gccgatggag agaacaatcg acaggccaat tgctgaccac
     1081 aaagagtaac ggccgccaac ccagtcccag aactcgaaca tgttggcagt atcaatacca
     1141 aactcgccaa cggctttggc attggtggaa agcgccgcaa agtgtttcgc aacgtgtttt
     1201 tcatcaccag ccgctttcag gaaccagtca cgcgcgctgt gggcgttggt catggtttcc
     1261 tgagtggtga aggttttaga tgctaccagg aacagcgtgg tttccgggtt tacttttttc
     1321 agcacttccg cgatgtgagt cccatcgacg ttagaaacaa agtgcatgtt caggtggttt
     1381 ttgtatggac gcagtgcttc agtcaccatg tacgggccga ggtcagaacc gccgatcccg
     1441 atgttcacta cgtcagtgat tgctttgccg gtatagcctt tccactcacc ggaaataatc
     1501 gcttccgaga aggttttcat cttctccagc accgcgttga cttctggcat tacgtctttg
     1561 ccatcaacca gaatcggggt attgctacgg ttacgcagcg ctacgtgcag cacggcgcgg
     1621 ttttcagtgc ggttgatctt ctcgccagag aacatcgact taatcgcgcc cgccagatcg
     1681 cactctttcg ccagatcttg taatttcgcc agcgtctctt cagtgatgcg gtttttggag
     1741 taatccacca gcatctgatc gtcgaatgtt gcggagaact tagaaaaacg atcgccgtct
     1801 ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat cgaagtgttt ctgtagtgcc
     1861 tgccaggcag cggtctgcgt tggattgatg tttttcatta gcaatactct tctgattttg
     1921 agaattgtga ctttggaaga ttgtagcgcc agtcacagaa aaatgtgatg gttttagtgc
     1981 cgttagcgta atgttgagtg taaaccctta gcgcagcgaa gcatttatta gctgaactac
     2041 tgaccgccag gagtggatga aaaatccgca tgaccccatc gttgacaacg gccccgctca
     2101 ccctttattt ataaatgtac tacctgcgct agcgcaggcc agaagaggcg cgttgcccaa
     2161 gtaacggtgt tggaggagcc agtcctgtga taacacctga gggggtgcat cgccgaggtg
     2221 attgaacggc tggccacgtt catcatcggc tacaggggct gaatcccctg ggttgtcacc
     2281 agaagcgttc gcagtcgggc gtttcgcaag tggtggagca cttctgggtg aaaatagtag
     2341 cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc ttgtgccaag gctgaaaatg
     2401 gatcccctga cacgaggtag ttatgtctga aattgttgtc tccaaatttg gcggtaccag
     2461 cgtagctgat tttgacgcca tgaaccgcag cgctgatatt gtgctttctg atgccaacgt
     2521 gcgtttagtt gtcctctcgg cttctgctgg tatcactaat ctgctggtcg ctttagctga
     2581 aggactggaa cctggcgagc gattcgaaaa actcgacgct attcgcaaca tccagtttgc
     2641 cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa gagattgaac gtctgctgga
     2701 gaacattact gttctggcag aagcggcggc gctggcaacg tctccggcgc tgacagatga
     2761 actggtcagc cacggcgagc tgatgtcgac cctgctgttt gtcgagatcc tgcgcgaacg
     2821 cgatgttcag gcacagtggt ttgatgtacg taaagtgatg cgtaccaacg accgatttgg
     2881 tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc gcgctgcagc tgctcccacg
     2941 cctcaatgaa ggcttagtga tcacccaggg atttatcggt agcgaaaata aaggtcgtac
     3001 aacgacgctt ggccgtggag gcagcgatta tacggcagcc ttgctggcgg aggctttaca
     3061 cgcatctcgt gttgatatct ggaccgacgt cccgggcatc tacaccaccg atccacgcgt
     3121 ggtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc gaagcggcag agatggcaac
     3181 ttttggtgca aaagtactgc atccggcaac gttgctaccc gcagtacgca gcgatatccc
     3241 agtctttgtc ggctccagca aagacccacg cgcaggtggt acgctggtgt gcaataaaac
     3301 tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc aatcagactc tgctcacttt
     3361 gcacagcctg aatatgctgc attctcgcgg tttcctcgcg gaagttttcg gcatcctcgc
     3421 gcggcataat atttcggtag acttaatcac cacgtcagaa gtgagtgtgg cattaaccct
     3481 tgataccacc ggttcaacct ccactggcga tacgttgctg acgcaatccc tgctgatgga
     3541 gctttccgca ctgtgccggg tggaagtgga agagggtctg gcactggtcg cgttgattgg
     3601 caatgacctg tcaaaagcct gcggcgttgg caaagaggta ttcggcgtac tggaaccgtt
     3661 caacattcgc atgatttgtt acggcgcatc cagccataac ctgtgcttcc tggtgcccgg
     3721 cgaagatgcc gagcaggtag tgcagaagct gcattttaat ttgtttgagt aaacacagta
     3781 tggcccggaa gacattattt cgggccgtat tgtttttctt gtcattatgc ccataaataa
     3841 acgagcctgt actctgttaa ccagcgtctt tatcggagaa taattgcctt taatttttta
     3901 tctctttctc taactaatta gagaaagaga taaatagtta agagaaggca aaatgaatat
     3961 tatcagttct gctcgcaaac gaattcccgt acactgaact ctctggctca ctgagggcac
     4021 catcatggca ctcccccgta ttacccaaaa agagatgact gaacgcgagc agcgtgaact
     4081 taaaacactg ctggatcggg cgcggattgc gcatggtcgc gtactgacca actccgaaac
     4141 caacagcatt aagaaagagt acatcgataa attaatggtc gagcgtgagg ctgaagcgaa
     4201 aaaagcccgc caattgaaga aaaagcaggc ttataaaccg gatcctgagg cgtcatttag
     4261 ctggtcagca aatacgtcga cgcgcggcag gcgctgatta acgccccttc tttttgcgcc
     4321 ccggcgaggt aaaacgctta ccgttggacg ccgggcgacc gcctttttcc gccgtttttt
     4381 ccatcttaac gactggacgt ttgatgccta ccgttttcgg tttggccttc gctttaggtt
     4441 ttgcctcgga agaggaattt tcgatgagct taaagaggtc gattaactca tcgtcggtta
     4501 aatcgcgcca ctcccccagc ggaatgccgc ttaaactgac gttcataatg cgcgtacgtt
     4561 ccagcttttt cacttcgtaa ccaaagtgct cgcacatgcg acggatctga cggttcagcc
     4621 cctgcaccag ggtaatgcgg aagacaaatg gcgcttcttt tttgactttg cactttttag
     4681 tcactgtccc gaggattggc acccccgccc ccataccacg aataaattca tcggtaatcg
     4741 gtttatcgac cgtcaccaga tactctttct catgatcgtt acccgcacgg aggatcttat
     4801 tcaccagatc gccgtgattg gtgaggaaaa tcagcccctg ggagtcttta tccaggcggc
     4861 caatcgggaa tacgcgtttg ctgtggttaa cgaaatcgac aatgttatcg cgctcgccat
     4921 cttcggtggt gctgacaata ccgacgggct tgttcagggc gataagtacc aaatcttcgg
     4981 cttcccgagg ttcaatcaac tgaccattta ctttcacaac gtcgccgggt ttcacctgat
     5041 cgccaatggt ggctcgcttg ccattaagga acacattgcc ttgctcgata tagcgatccg
     5101 cttcgcggcg tgagcaaatt ccgctttcgc tgatgtattt atttaaacgg actgatgagt
     5161 cgggcagcat aaattctcct gtaaaacgcg gaatataccg tacctcttag cggggaaaaa
     5221 cagggttatg cggcaacgtc gaaattaaat gtgatctgcc acgcctttta tcacaaaagt
     5281 gtctccggaa gtacgatgca aatttgcctg gcaaaataag gataatcaac atattacgaa
     5341 tcgctgcaca cctgtgctga tgactactac gcagagaaac aaatgtgcaa gagaaagcgc
     5401 tatttctccc catgcaaaga gtgaaaaatc gctttgaaag gccctcatga tggggttata
     5461 tcagatcaag cggtgagaaa gcgtatttgc acaaatgtgc agaaccagga ggcggcaatg
     5521 gaaaacagtg acgatatccg tttgattgtg aagattgccc aactctatta cgaacaggat
     5581 atgacgcagg cgcaaatcgc gcgcgaactg ggtatttacc gcaccaccat cagccgcttg
     5641 cttaaacgag gccgcgatca gggaattgtc accatcgcca tcaactatga ctacaacgaa
     5701 aatctctggc tggagcagca actgaagcaa aagtttggcc tgaaagacgt tgtggtggtg
     5761 tcaggaaatg atgaggatga agagactcaa ctggcgatga tggggttaca cggcgcgcaa
     5821 ctgctggatc gcttgctgga acctggcgat attgtcggtt tttcctgggg ccgcgcggtg
     5881 aacgcactgg ttgaaaactt gccgcaggcg gggcaatcgc ggcagttaat ctgcgtacca
     5941 attattggcg gaccgtccgg taaactcgaa agccgctatc atgtaaacac attaacctac
     6001 agcgcggcag cgaagttgaa aggggaatcg catctcgcgg attttccggc tctgctggat
     6061 aacccattaa ttcgtaatgg gatcatgcag tctcagcact ttaaaaccat ctctgcctac
     6121 tgggataatc tggatgtcgc cctggtggga attggctcac cggccattcg cgacggcgct
     6181 aactggcatg cgttttatgg tggtgaagag agtgacgacc tgaatgcccg ccaggttgct
     6241 ggcgatattt gctcgcgctt ttttgatatt cacggcgcaa tggttgaaac aaatatgagc
     6301 gaaaaaacac tctctatcga aatgaataaa ttaaagcagg cgcggtattc cattggcatt
     6361 gccatgagtg aagaaaaata cagcggaatt attggtgcac tgcgtggaaa atatattaat
     6421 tgtctggtaa cgaatagcag cacagctgaa ctattactga aataaaaatc ttcccgatgt
     6481 ttaccgtaat aaaaattacg gcgggtctct attatctaaa atcaggagtc tgttatgcaa
     6541 acgtggttaa atttgcagga taaaatcatt attgtcaccg gtggcgcatc cggcattggt
     6601 ctggcgattg tggatgaatt attagcacaa ggcgcgaatg tacagatggt cgatattcac
     6661 ggtggcgatg gacaatatga aggtcataaa ggctatcagt tttggccgac cgatatttcc
     6721 agcgccaaag aggtaaatca tacggtagcg gaaattatcc agcgttttgg tcgcatcgac
     6781 ggtctggtca ataacgccgg ggtcaatttc ccgcgtctgc tggtcgatga gaaagcgcct
     6841 gccgggcagt atgaactcaa cgaagctgca ttcgaaaaaa tggtcaatat caaccagaaa
     6901 ggcgtttttc tgatgtcgca ggcggtggcg cgacagatgg tcaaacaaca tgatggcgtg
     6961 attgtgaatg tttcctcaga aagtgggctg gaaggctcag aaggccaaag ctgttacgcc
     7021 gctaccaaag ccgcgcttaa tagcttcacg cgatcctggt cgaaagaatt gggtaaacac
     7081 ggtatccgtg tagtcggtat cgcgccgggg attctggaaa aaaccaggct acgcacgccg
     7141 gaatatgaag aggcgctggc gtggacgcgc aatatcaccg tcgagcagct gcgtgaaggc
     7201 tataccaaaa acgccattcc tattgggcgc gccggaagat tagcagaagt cactgatttt
     7261 gtttgttatc tgctgtctca acgcgccagc tatatcaccg gagtaaccac taacattgcg
     7321 ggcggcaaaa cgcgcggcta aggaggcttt atggtcaacg ccattttttg tgcccacggc
     7381 aaattagctt gcgccatgct ggaatcggtt cagatggttt acggcaacgc caatgttgaa
     7441 gccgttgaat ttgtgcccgg tgaaaacgcc ggagacatcg tagcaaagct ggaaaagtta
     7501 gtaagcattc acaatcaaga tgagtggctg attgccgtgg atttacagtg cggcagcccg
     7561 tggaatgcgg cagcgatgct tgcgatgcgc aatcctcgtc tgcgggtaat cagcggcctt
     7621 tctctgccgc tggcgctcga actggttgat aaccagcaca gcatgaatgt tgatgaactg
     7681 tgtgaacacc tgacgcaaat cgcaaagcaa acctgcgttg tctggaaaca actggcaacg
     7741 gcagaggagg atttctgatg aacattacgc tcgcccgcat tgatgaccgt ctgatccacg
     7801 gccaggtcac taccgtctgg tcgaaagtgg ctaatgccca gcacattatt atctgcaatg
     7861 acgaagttta taacgatgaa gtgcgccgga cattattgcg tcaggctgcg ccaccgggaa
     7921 tgaaggttaa cgtggtcaat attgaaaaag ccgtcgcggt ttatcacaac ccgcaatatc
     7981 aggacgaaac cgttttttat cttttcactc gcccacagga tgccttagcg atggttcgcc
     8041 agggggtaaa aatagacacc ctgaatattg gcggcatggc ctggcggccc ggtaaaaaac
     8101 agttaaccaa agcggtttca ttagatgatg atgatattaa cgcatttcat gagttaaata
     8161 atcttggcgt catactggat ttacgcgtcg tagcgtcaga tccctcaatc aatattatcg
     8221 acaaaataaa cgaacagtta atcgcaaatt aaataatagc gcctgtgtac atatgcttta
     8281 acaggcaggg atgacgacac ttaaaaggtg acaaatcatg gaaataagta ccctacaaat
     8341 cattgctata tttctttttt cctgtattgc cggaatgggc agcgtgctgg atgaatttca
     8401 gactcatcgt ccgttaattg cctgtacggt gattggttta attctcggtg atttaaaaac
     8461 cggaattatg ctcggtggta cgctggaatt gatagctctc ggctggatga acgtcggcgc
     8521 ggcgcaatct ccggattctg cactcgccag cataatctcc gccattctgg ttatcgttgg
     8581 tcagcaaagc atcgccaccg gaatcgccat cgcgttgcct gtggctgcgg caggccaggt
     8641 gctgacggtg tttgcccgta ccatcaccgt agtgttccag cacgcggcgg ataaagcagc
     8701 ggaagaagca cggtttcgca ctctcgatat tctgcatgtc tccgcgcttg gcgtgcaggc
     8761 actgcgcgtt gctattccgg cactgattgt ctcgctgttc gtcagcgctg atatggtgag
     8821 caatatgctg agcgccattc ccgaatttgt gacccgtgga ctgcagattg ctggcggttt
     8881 tatcgtggtg gtcggttacg ccatggtgct tcgcatgatg ggcgtgaaat atttgatgcc
     8941 tttctttttc ctcggtttcc tcgcaggtgg ctacctcgat ctcagtctgc tggcgttcgg
     9001 tggcgtcggc gtgatcatgg ccctgctcta catccagcta aatccacagt ggcgtaaagc
     9061 tgaacctcaa ctccaggcca ccgcctccac cgcccttgac caacttgacg attaacggag
     9121 ccatcatgga acagagaaaa attacacgca gcgatctggt gagcatgttt ctgcgctcca
     9181 acctgcaaca ggcgtccttt aactttgaac gtattcacgg gctgggtttt tgctacgaca
     9241 tgatccccgc catcaagcga ctttacccat taaaagagga tcaggttgcg gcgctcaggc
     9301 gacacctggt gttcttcaat accacgccag ccgtatgtgg cccggtcatc ggcgtcactg
     9361 ccgccatgga agaggcgcgg gccaacggcg cggaaattga tgacggtacc attaacggca
     9421 tcaaagtcgg tctgatggga ccattggcag gagttggcga tccactggtc tggggaacgc
     9481 tgcgcccgat taccgccgcg ctcggcgcat ctctggcact ttcgggcaac attctcggcc
     9541 cgctgctgtt tttctttatt ttcaatgcgg tgcgtctggc gatgaagtgg tatggcctac
     9601 agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat gggcgggaat gtgctgcaaa
     9661 aactcaccga aggcgcgtcg attctcggac tgtttgtgat gggcgtgctg gtgaccaaat
     9721 ggacgtcaat caacgtaccg ttgctggttt cacaaacgca tgccgccgat ggctccaccg
     9781 tcaccatgac cgtgcagaac attctcgacc aactttgccc tggtttgctg gcgctcggtc
     9841 tgacgctgct gatggttcgt ctgctcaaca aaaaaattaa cccggtatgg ctgattttcg
     9901 ccctgtttgg tttagggatt atcggtaatg cgctgggctt cctgtcctga ttcttcgccc
     9961 cggcacgact gccggggtca tcgctcaaca tgaggtggtt tatgaaaaca acagctctgc
    10021 gtctttatgg caaacgtgac ctgcgtctgg aaacctttga ccttcctgaa atgcaggagg
    10081 atgaaatcct cgcaacggtg gtcactgaca gcctgtgcct ctcttcctgg aaagaggcca
    10141 atctgggtga aaaccataaa aaagtacccg acgatgtggc gaccaacccc atcatcatcg
    10201 gccacgagtt ttgcggcgat attctggccg tgggtaaaaa gtggcagcac aaattccagc
    10261 cgggtcagcg ttatgtgatt caggccaacc tgcaactccc cgaccgcccg gattgccccg
    10321 gctactcctt cccgtgggta ggcggcgaag cgacgcatgt ggttattccc aacgaggtca
    10381 tggaacaaga ttgcctgctg gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg
    10441 aaccgctttc ctgcgtgatt ggcgcgttca acgccaacta tcatcttcag gaaggtagtt
    10501 ataaccacac gatggggatt cgcccgcaag ggcgcacgct gatcctcggc ggcaccggcc
    10561 cgatggggct gctggcgatt gattatgcgt tgcatggacc ggttaacccg tcgctgctcg
    10621 tcattaccga taccgacaac gacaaattga gttatgcacg caagcactat ccatcagaac
    10681 cgcaaacact gattcattat ctcaatgccg ccgatgcagc atttgatacg ttaatggcac
    10741 tgagtggcgg tcacggcttc gatgatattt tcgtctttgt gcctaatgaa ggactggtga
    10801 ctctcgcctc ttccttgctg gcgacagatg gttgtctgaa tttcttcgcc ggaccgcagg
    10861 ataagcactt cagcgcgcca attaatttct acgatgtgca ttatgcattt acccactacg
    10921 tgggcacgtc aggcggcaat accgacgaca tgcgcgcagc ggtcaaattg attgaagaga
    10981 aaaaagtgca ggccgcaaaa gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa
    11041 ccacgcttga attgcctgcc atcggcggcg gcaaaaagct ggtgtatacc gggaaatacc
    11101 tgccgcttat gtcactcacg cagattcagg atcaagcact ggcggcaatt ctggcgcgtc
    11161 atcaggggat ctggtccggt gaggcggagc aatacctgct cgcccatgca gaggcaattt
    11221 cccatgatta atcgcgctac ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc
    11281 gcgacctgat gtgcggctga ttgctgaagc ggcacaatgt gcacgagaag gataaaaaaa
    11341 catcagcgcc ttaaggggcg ctcaaattgg tggcaaagag cacattgttt atgccggatg
    11401 cggaatgaag ccttattcgg cctacataat cttgaaaatt caatatattg cagttatctg
    11461 gtaggccagg taagcgtagc gcatcaggca atcctgtgtt tgtcatcagt ctcagcgcct
    11521 gtgcaatatg tgacatcgtg agccatgtca gtaagtggtt aaattcgcag tggtagcatt
    11581 ccttccgtgt tttcttacta accggagaag taatggaact gcttttattg agtaactcga
    11641 cgctgccggg taaagcctgg ctggaacacg cgctgccgct gattgctgaa cagttgcagg
    11701 gtcgccgttc tgcggtattt attcctttcg cgggcgtaac gcagacctgg gatgattaca
    11761 cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc tgtcaccggt attcatagcg
    11821 ttgtcgatcc cgttgccgcg attgaaaatg cggaaattgt gattgtcggc ggcggtaata
    11881 ctttccagtt gctgaaacag tgccgcgagc gcggactgct ggcaccaatt actgacgtgg
    11941 ttaaacgtgg cgcgctgtat attggctgga gcgctggcgc taaccttgct tgcccaacca
    12001 ttcgtaccac caacgatatg ccgattgtcg atccacaagg tttcgatgcg ctgaatctgt
    12061 tcccgttgca aatcaacccg cacttcacca acgcgctgcc ggaaggccat aaaggtgaaa
    12121 cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc agaactcaca attattggtc
    12181 taccggaagg taactggatc acggtgagta aaggtcacgc tacgctgggt ggcccgaaca
    12241 ccacttatgt gtttaaggct ggtgaagaag cggttccgct ggaagcgggt caccgttttt
    12301 aagtgaattg ccggatgcat gatccggcat cttgtacata gtgcctgatg cgacgcttta
    12361 cacgtcttat caggccttca gggtcacggt attaatactc gtcccgtcct tcgtcttcat
    12421 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcaga cgctgcatat
    12481 ctcccagtaa gcctaaatgt agcgaactgg tttcaatact ctgcacgttt tgctgatgca
    12541 ggcgatcgac gtgagcatgg gaataacggc gattaagaat gcgaaagcga tgcttgctgc
    12601 ggcgcaaacg acgggcgctg gtaacatcgc cagagaagaa aaccgacatt gccagtttta
    12661 aattactgag caattgctca tagagcgcat ccagttcctt caagccatca agcgaaaatg
    12721 cccgccgtgc tgccagcgat ttatcggcaa tttcactccc catgcgctcg acgatatcgg
    12781 aggcctgttc aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttctt
    12841 ctgccagttc ctcttttggc atccgcgcca gatacagctt aatggcggta tagagaacgt
    12901 tgatgtcatc agccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
    12961 tattcaaccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgggcag
    13021 cgttcgccag agcaagcgtg ggcgtatcca gcgcgctgac atccagatgt ttaggccgta
    13081 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
    13141 caacaaatgg cagcatgacc aggcagcgta caaggttgta gaagacgtgg aaatagatca
    13201 ccaattccgc ttttggcaat ggcaacttcc ccattgtctc tgccagcaaa tgaacaaacg
    13261 gcaggataat caggctaccc accagcttaa acagcaggct acccagcgcg acacggcggg
    13321 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggtttgcac
    13381 caatcaccag acaaagcgcc actgggaaag agataatgcc tgcggcggtc agagtcgcgg
    13441 tcagcagtac cgcggcaagg ctggagtagc tgataatggc gaacatcgca ccaatcagcg
    13501 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
    13561 tgatcggcgt tacggcctgc acaatcaact ccagcgccag caaaatcaac ccaagaccaa
    13621 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
    13681 cgataaaaat aagtaatggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
    13741 gcgccgtccc gacatctgca cccagcacaa tcaccagagc cggtgcgagg gctaccagat
    13801 cctgagcgac gaacgaggtc accagcatag tggtggcatt actgctctgc accagtgcgg
    13861 taacgcctat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
    13921 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
    13981 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca ggttctcctt
    14041 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
    14101 tctttactgt gccttttgac cttcgttttt ccggctaaga ttattccagc ctgtttccag
    14161 tataagtgtt tcatattaat aaagagacag ggcactcatt gagcgccctg tttgttgtaa
    14221 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
    14281 gctcaacatc tgagacgctc atacctttac ggcgggcata gtcttcaacc tgatcgcgct
    14341 ggatttgcgc caccgcgtaa tacttgctgt ccgggtggct gaagtaccaa cccgaaaccg
    14401 atgcaccagg ccacatggcg aaagattctg tgagtttcat gccagtgtgt ttttccactt
    14461 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccgga taacccggtg
    14521 ccggacggat gccctggtag ttttcgcgga tcagctcttc gttgctgagg ttctcgttcg
    14581 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
    14641 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
    14701 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
    14761 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaagccgg
    14821 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggta cgcgtttcgt
    14881 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
    14941 gcggattcag cgttttttcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
    15001 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgcggatac ttcccggaca
    15061 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg
    15121 cttcgacttc ctgcacaccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
    15181 aagcgaagtc gttatcacgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttctttc
    15241 gtccgtgctg aatacgcacg gtttcgtact ccttgcgggt acgagcaacg aaatcatcgc
    15301 gctgagtgtc ggaaagcaac gccgccacca caccaacggt gcgtgaagcg ttctgcacat
    15361 acaccgtcgg gccgctgtag ttctgctcaa ttttcaccgc cgtgtgcgct tttgaggtgg
    15421 tcgcgccgcc aatcaatagc ggaatggtga agccctgacg ctccatctct ttcgccacgt
    15481 taaccatctc gtccagcgac ggcgtgatca gccccgaaag gccaatcaaa tcagcattca
    15541 cttctttagc ggtacggaga attttttccg caggcaccat cacgccgaga tcgacaattt
    15601 cgtagttgtt acattgcagc accacgccaa cgatattttt accgatgtcg tggacatcgc
    15661 ccttcactgt ggcgatcacc atcttgccgt tggttttacc ctgctctttg ctggcttcaa
    15721 tgaacggctc gagatacgcc accgcctgtt tcatgacgcg cgccgatttc accacctgcg
    15781 gcaggaacat tttgccctcg ccaaacaggt cgccgaccac gttcatgccg tccatcaacg
    15841 ggccttcaat cacttcaatc ggacgcgtag cctgctggcg ggcttcttcg gtatcctgct
    15901 cgataaactc ggtaatgcct ttgaccagcg agtattccag acgtttattc acttcccacg
    15961 agcgccactc tgcctgctgg gtgttagcgg tgtcgtcggt tttgctgccg cgatatttct
    16021 cggcaagctc gagtaaacgc tcggtgccat cgtcgcgacg gttgaggatc acatcctcca
    16081 ccgcgtcgcg tagctcggta ggcaggtcgt cgtaaatcgc cagttgcccg gcgttgacga
    16141 tccccatatc catgccattg cgaatagcgt agtagaggaa caccgcgtga atggcttcgc
    16201 gcaccggatc gttgccacgg aatgagaaag aaacgttaga aacgccgccg gaaatcagtg
    16261 cgtgcggcag ttcacgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtaattgt
    16321 tgtgctcttc aatgccagtt gcgaccgcga agatgttcgg gtcgaagatg atatcttcag
    16381 ccgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
    16441 gtgcgcgggt atcggcctgg ccctgttcgt caaaagccat taccacgact gccgcaccgt
    16501 agcgacgcaa cagtttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
    16561 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaataacg tcccattttg
    16621 aggagtcgat cattatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
    16681 aacgcaccat cgccgcttcg gcgtcgagca tcccttcatc catgttgata tcgataatct
    16741 gcgcaccgtt ttccacctgc tgacgcgcca catccagcgc ctcgctgtat ttctcttctt
    16801 tgatcaggcg cttgaactta gcggaaccgg tgacgttggt gcgttcaccc acgttcacaa
    16861 acaggctatc ttcgccaatg ttcagcggct ccaggccgga caaacggcag gctacgggga
    16921 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgcg gcaatatgtt
    16981 gtggcgtggt gccacagcag ccgccgacga tattgagaaa gcccgcttgc gcccattcac
    17041 gtatctgttt tgccatcgtg tcggcgtcga gatcgtactc accaaaggcg ttgggtagcc
    17101 cggcgttcgg gtgcgctgtg acgtagcatt ccgcaatccg tgataattcc tgcacgtact
    17161 ggcgcagttc gtctggcccc agcgcacagt taaggccgaa agtcagcgct tcggcgtggc
    17221 gcagagagtt ataaaacgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
    17281 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
    17341 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc agaatcaggt
    17401 cggcaccacc ttccaccagc gctttggtgg actctcgata agccgccacc agcccgtcaa
    17461 aagtgatatt acgaaatgcc ggatcgttga catccggaga aatagacgcc gtgcggttgg
    17521 tcgggccgag aacaccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcat
    17581 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac aggaattcca
    17641 tctggtaatc cgccatcgca atggttgtgg agttgaaggt gttggtttcg atgatatccg
    17701 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggtttg ctgagcacca
    17761 gcaggtcgtt gttgcctttg agatcgcatg gccagtcggc aaagcgttca ccacgaaaat
    17821 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc agcaccagaa
    17881 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
    17941 cgctcaaccc agatttaata aaaattcaac aaaccatact ggcataaacg catctgtggt
    18001 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
    18061 aactattgca ttagctaaca ataaaaatga aaatgatttc cacgatacag aaaaaggaga
    18121 ctgtcatggt cgcacccatt cccgcaaaac gcggcagaaa acccgccgtt gccaccgcac
    18181 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
    18241 aatccaatgg cagtgtggca ctcacggaac tggcacagca agccgggtta cccaattcca
    18301 cgacccaccg cctgctaacc acgatgcagc aacagggctt tgtccgtcag gtcggcgaac
    18361 tgggtcactg ggcaatcggc gcacacgcct ttatggtcgg tagcagcttt ctccagagcc
    18421 gtaatttgtt agcgattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
    18481 cggtcaatat ggcggtgctt gatcaaagca atcacgaagc gattattatc gaccaggtac
    18541 agtgtactca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
    18601 ccggtgcggg taaagccttt ctggcccaac tgagcgaaga acaggtgacg aagctgctgc
    18661 accgcaaagg gttacatgcc tatacccacg caacgctggt gtctcctgtg catttaaaag
    18721 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
    18781 ggctgcgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
    18841 caatttccgg accgatttca cgtattaccg atgaccgcgt gaccgagttt ggcgcgatgg
    18901 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatacgctga ctttttcgcc
    18961 tctgatttat acgaatcgaa cgctaaaccg ctgcttgcgc cgatacgcat aaacatcttc
    19021 cacatgccct tcacgtatgc ggttttgtag tgcgcgccag taatcagcgc ggaacaagtc
    19081 ggcgtgcatc tcttcaaata gcggaccaat acgcgggtcg gcacataacc agtgacggaa
    19141 ctcttccggg aaaacatcgc ccggcgagac gctgtaccac ggttcgctgg caagttcgtc
    19201 ctccgggtag cgcggcggcg ggatgtcgcg gaagttcact tccgtcatgt agcaaatttc
    19261 gtcgtaatca taaaacacca cgcgcccgtg acgggtgaca ccgaagtttt taaacagcat
    19321 gtcgccaggg aaaatattag cggcggcaag ctggcgaata gcgttaccgt attcttcaat
    19381 ggcatcgcgc aactgctgac cttccacttg ttccagccag atattgagcg gcaccatccg
    19441 ccgctcaata taaagatggc gaatcacaat ttgttcgccg agatcggtga ttttttccgc
    19501 tgcttcctgg agcagtaatg ccattaatgc cggggaaata tgccgcttct ccagcacaaa
    19561 gttttcaaac tcctgggtgt ccgccattcg gcccacgcga tcgtgctctt tcaccagttg
    19621 atagcaggca cgaacgtgag cggcagacat ctctttctgc ggcgcgaact tgtctttgat
    19681 gactttgaat acccgatcaa agcccggcag cgtaaacacc agcatcacca tgccacgaat
    19741 acccggcgct tcaatgaact gctcattaca gccctgtaga taaacgagat attcgcggta
    19801 gctttcggtt ttggcgtgct tctggcagcc gatagccata tacaattcag cggtggtttt
    19861 acctggcaga atttcccgta gccactcgac cagcgctgcg ggcagcggtg cataaaccat
    19921 aaaataagaa cgcgcaaagc caaaaacaat gctcgcttcg gcggtggtcg tcaggcaggt
    19981 atcaataaat aactcgccgt cgtccgtctg gtggatcggc agcaaaaatg gcaatgtgcc
    20041 ggaaggcgtg atcagattgc ctaccagcca ggcggcttta ttgcggtaaa acagttcgtt
    20101 cgccacctgt aaatgacttt ccgcgaggtt gtctgtcccc agcgtttccg tcagatggcg
    20161 aacgatgtaa tgaatgtcac ggcttttatt ctgccaacgc agacgcagcg gcagatcgct
    20221 gataacgcgc atcagcagag attcccagcc gtgatcgggg tgaaagtctt tcgccagcgg
    20281 gcggggaatg gtacgaaaac ggcgctctgg ctgggagcta aagataaaaa gccgctcggg
    20341 agtaagcgag cggtggtcaa ataacctaca gtacacggag ttgaaaaagc tctccgcaat
    20401 ctcgaagcgc gggtaatccg gcaacagccg cgtgtaatgc tctttgacgc gcagtaaaaa
    20461 tgccgcgtcc gtgctttggc cgttggtaat gcagcgcagt tgctccacga ccagacccac
    20521 gtgatgatcg taaagatgaa tacggttttt catcgcctgc tggacagcat gccagtcggc
    20581 ttgctcgaaa cgctgctgcg ccccggaagt cacttcgagg aatcgaccat actgagcgtc
    20641 gaaaccttgc aaaatggttt gtgcaatcag taattccagg ccacgcggca tttgttgccc
    20701 tcgtcgggag cagagaattg ccggataaga cgcgtaagcg tcacatcagg cgctgattgc
    20761 cggatgcggc gtagacgcct tatccggcct acgttcggca acggctgtag gcatgataag
    20821 acgcgcaagc gtcgcatcag gcgctgattg ccggatgcgg cgtgaacgcc ttatccggcc
    20881 tacagtcagc aacgtttatc ggttgcttag aactgcgatt cttcggtgga gccagtcagt
    20941 gcggtaaccg acgacgtgcc gccctgaata atggtcgtca ctttatcgaa gtaacctgta
    21001 cccacttcct gttggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc
    21061 tgctgcactt tctcaacata gtgcttcata ccctcgccct gggcataggc gtttgccagg
    21121 tcaaacatgt tgaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag
    21181 cccatatccg acagctgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc
    21241 cagttgaacg acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggatg
    21301 gcttgtgcaa agcgacgcgc cagttccagg tccggcgtgg aggtttcaca ccagaccagg
    21361 tcagcatatg gcgcatacgc caggccacgg ctgatcgctt gctcaatacc cgcatgagta
    21421 cggaagaagc cttcactggt acgctcgcca gtaataaatt cgctgtcata tgggtcgcaa
    21481 tcggaggtaa tcagatccgc cgcatcggca tcggtacgag caaccagcag ggttggaacg
    21541 cccgtcacat cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc
    21601 accaaaactt tgccgcccat gtgaccacat ttcttcactg atgccagctg atcttcgaag
    21661 tgaactgccg ctgcaccggc ttcaatcatc gctttcatca gttcaaaggc attcagtaca
    21721 ccgccaaaac cggcctccgc atcggcgacg atcggcagga agtaatcgac atagcgcgga
    21781 tcgcccggct caatgcccgc ggaccattgg atctgatccg cacggcggaa agtattgttg
    21841 atccgctcca ccaccgctgg caccgagttt gccggataga gcgactgatc cggatacatg
    21901 ctggccgcca ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata
    21961 cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag
    22021 ccttttttcg actcgccgtg cagcagacgc cacattttcg ccgcgcccag ttgcgccagg
    22081 gtgcattccg gattgactga accgcgtaat ttcaccacat cttccgcgct gtacgggcga
    22141 gtaatgcctt cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta
    22201 cgggttttca tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc
    22261 ctggcagggt caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg
    22321 cggcatcgtc aaaacgcccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt
    22381 tcatctcttc gcccagcatc tggcggaaca aggctttagt caccggtttg ccattgctca
    22441 acgttttttg atgatggatc cactgccaga ttgaggtacg ggaaatttca gccgtcgccg
    22501 catcttccat caggccataa atcggcacac agccattgcc agagatccac gcttcgatgt
    22561 actgcacagc cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag
    22621 gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc gcgcatcact tcaagctgat
    22681 ttttacggga gccgagaatg tcgttgaata ccgccattgc cgtatccgca aggcctgggt
    22741 gggcaatcca tgtgccatcg tgaccgttat tagcttccag cgatttatcc gcttttactt
    22801 tgttgagcac ctggttattg cgctcttcat ctttgctcgg aataaatgcc gccataccgc
    22861 ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcaa caggcgtgag taagcattca
    22921 ggaagggttt atccatcgtc actgcctgtc tatctggcag gacgcgatcg ggatagtttt
    22981 tcaatgtttt gatataactg aagatgtaat cccaacgacc gcagttcaga ccaacaatat
    23041 ggtcacgcag cgcgtgaagg atttcatcca tctggaacac ggcgggcagc gtttcaatca
    23101 gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga
    23161 cttcgctcca ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac
    23221 cgctgccctt tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca
    23281 ggctaccggg gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc
    23341 gtacccgaca aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat
    23401 tggtatagct gatggtgccg ttaaccgcat cgcgcaggtt aatttgccca tcgatcactt
    23461 tgttccagtc tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt
    23521 tgagcgcgtt gatcaccatc ttgcgctcaa ccgggccggt aatctctacc cgacgatctt
    23581 ctaagtccgc aggaatcccg cgaattttcc agtcagcatc acgaatggaa gctgtttccg
    23641 aaataaaatc aggcaacgtt ccgttatcga tatcctgctg ctgctgaatg cgcgctgcca
    23701 gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt
    23761 cggcagtaag aatatgcttc tcctgctcgc catacggcct tgtgaaagcc aattcatcgg
    23821 ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttccccac
    23881 tgtgaacgaa ggatcgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa
    23941 tgcaaaatca aaaacaattt ccattttaat
//
LOCUS       DAZKJR010000078        23837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKJR010000078 DAZKJR010000000
VERSION     DAZKJR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.078"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..289
                     /locus_tag="ACHOWS_003050"
     CDS             <1..289
                     /locus_tag="ACHOWS_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0048594.1"
                     /translation="SVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEIS
                     WRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(332..1483)
                     /gene="ydiO"
                     /locus_tag="ACHOWS_003051"
     CDS             complement(332..1483)
                     /gene="ydiO"
                     /locus_tag="ACHOWS_003051"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFQ0048595.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1497..3092)
                     /gene="ydiF"
                     /locus_tag="ACHOWS_003052"
     CDS             complement(1497..3092)
                     /gene="ydiF"
                     /locus_tag="ACHOWS_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFQ0048596.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3238..3996)
                     /gene="aroD"
                     /locus_tag="ACHOWS_003053"
     CDS             complement(3238..3996)
                     /gene="aroD"
                     /locus_tag="ACHOWS_003053"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0048597.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDIIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4027..4893)
                     /gene="ydiB"
                     /locus_tag="ACHOWS_003054"
     CDS             complement(4027..4893)
                     /gene="ydiB"
                     /locus_tag="ACHOWS_003054"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFQ0048598.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4905..6170)
                     /gene="ydiN"
                     /locus_tag="ACHOWS_003055"
     CDS             complement(4905..6170)
                     /gene="ydiN"
                     /locus_tag="ACHOWS_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0048599.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFATFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6397..7611)
                     /gene="ydiM"
                     /locus_tag="ACHOWS_003056"
     CDS             complement(6397..7611)
                     /gene="ydiM"
                     /locus_tag="ACHOWS_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0048600.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALLLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(7711..8067)
                     /gene="ydiL"
                     /locus_tag="ACHOWS_003057"
     CDS             complement(7711..8067)
                     /gene="ydiL"
                     /locus_tag="ACHOWS_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFQ0048601.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTDDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTVFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8203..8310)
                     /gene="rprA"
                     /locus_tag="ACHOWS_003058"
     ncRNA           complement(8203..8310)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOWS_003058"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8496..9608)
                     /gene="ydiK"
                     /locus_tag="ACHOWS_003059"
     CDS             complement(8496..9608)
                     /gene="ydiK"
                     /locus_tag="ACHOWS_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFQ0048602.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            9995..13051
                     /gene="ydiJ"
                     /locus_tag="ACHOWS_003060"
     CDS             9995..13051
                     /gene="ydiJ"
                     /locus_tag="ACHOWS_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFQ0048603.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSSMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWVAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13048..13458
                     /gene="menI"
                     /locus_tag="ACHOWS_003061"
     CDS             13048..13458
                     /gene="menI"
                     /locus_tag="ACHOWS_003061"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFQ0048604.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13558..13746
                     /gene="ydiH"
                     /locus_tag="ACHOWS_003062"
     CDS             13558..13746
                     /gene="ydiH"
                     /locus_tag="ACHOWS_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFQ0048605.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14295..14663
                     /gene="sufA"
                     /locus_tag="ACHOWS_003063"
     CDS             14295..14663
                     /gene="sufA"
                     /locus_tag="ACHOWS_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFQ0048606.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14672..16159
                     /gene="sufB"
                     /locus_tag="ACHOWS_003064"
     CDS             14672..16159
                     /gene="sufB"
                     /locus_tag="ACHOWS_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0048607.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16169..16915
                     /gene="sufC"
                     /locus_tag="ACHOWS_003065"
     CDS             16169..16915
                     /gene="sufC"
                     /locus_tag="ACHOWS_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0048608.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16890..18161
                     /gene="sufD"
                     /locus_tag="ACHOWS_003066"
     CDS             16890..18161
                     /gene="sufD"
                     /locus_tag="ACHOWS_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0048609.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18158..19378
                     /gene="sufS"
                     /locus_tag="ACHOWS_003067"
     CDS             18158..19378
                     /gene="sufS"
                     /locus_tag="ACHOWS_003067"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFQ0048610.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19391..19807
                     /gene="sufE"
                     /locus_tag="ACHOWS_003068"
     CDS             19391..19807
                     /gene="sufE"
                     /locus_tag="ACHOWS_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFQ0048611.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19956..20960
                     /gene="ldtE"
                     /locus_tag="ACHOWS_003069"
     CDS             19956..20960
                     /gene="ldtE"
                     /locus_tag="ACHOWS_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFQ0048612.1"
                     /translation="MKCASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21024..21260)
                     /gene="lpp"
                     /locus_tag="ACHOWS_003070"
     CDS             complement(21024..21260)
                     /gene="lpp"
                     /locus_tag="ACHOWS_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFQ0048613.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21571..22983)
                     /gene="pykF"
                     /locus_tag="ACHOWS_003071"
     CDS             complement(21571..22983)
                     /gene="pykF"
                     /locus_tag="ACHOWS_003071"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFQ0048614.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(22991..23185)
                     /locus_tag="ACHOWS_003072"
     CDS             complement(22991..23185)
                     /locus_tag="ACHOWS_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048615.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            23540..23749
                     /gene="fumD"
                     /locus_tag="ACHOWS_003073"
     CDS             23540..23749
                     /gene="fumD"
                     /locus_tag="ACHOWS_003073"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFQ0048616.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         6200 a         5720 c         6005 g         5912 t
ORIGIN      
        1 cagcgtcagt tatatttgcc atgaatttac caaagagtat cgcatttccc ctattaacta
       61 tgttattcaa cggcgtatga cggaagcgaa atggtcactc actaatactg aattatcaca
      121 ggcagagatc tcctggcgtg tgggttatga aaatgtcgat cactttgcca aactgttttt
      181 gcgccatgtc ggctgttcgc ccagcgatta ccgcaggcaa tttaaaaact gttttgcgga
      241 acaagaaatc ctatctgaat ttcctcaacc agtaagtctt gccggataaa tctggcggcg
      301 cagtttgtta agaagctgcg ccgcctgcag attatttgtt ctgataatct ttcaggatct
      361 gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt tcacaacgga
      421 catcacgcca gaagcgggag acgcgcgcct catcggtata gcccagaccg cccatgattt
      481 gaatcgcatc atcaatgact tccattgcgg tacgtgcgca gtacagtttt gccagcgccg
      541 cgctggtgcg cagtgactga tgctgatcgg cttgccatgc cactttcaac accatgttgc
      601 gcatgttgtc gatcttaatc gccatcagcg ccagtttttc ctggatcatc tggttatgac
      661 caatgggctt accaaaagcg atacgctggt tggcatagcg ggcggcatct tcgaatgcgc
      721 attcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca
      781 tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca acttccacgt
      841 tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc agtggattga
      901 ttttaatgcc gggcttactg gagtcgaccc accacagagt aaaggccttt ttcggatctt
      961 tcggttgcgg atcgcgcgcc aacaccagca tatatggata ctctttcgca ccggtaataa
     1021 aggttttctg tccgttgatg taaaccttgc cgtttttacg cgtgtaagtg gtagtggcac
     1081 tgttgttgtc tgagcctgcg cctggctctg tcaatgccag ggcataggcg ggatcacccg
     1141 tttccagggt gctttccgcc gttttacgta gctgctctgc agaaccgaaa cggcgcatac
     1201 tgtgaataca ttgaccgttg gtgatcaaaa atgccggggc accgcatttt gacacttcca
     1261 tcagcgccag catttgggtg acgtaatccg cagggatacc accaaattct tccggcacgc
     1321 caagcatgga aataccgtta tccgccagtg cccgcataaa ttcacgcggg tatgtcccgt
     1381 tttgatcgca ggtgcggaaa tactcttccg gaaagttagt cgtaatcagt tcgcgaatac
     1441 tggccagcag cagttcttgt tcttcagtta aagaaaaatc catcattata ctcctgttaa
     1501 tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct ttcgtccatc
     1561 agtttgagtt ctggcgaaat cactggggtg aaatccattt tgtcgagaat atctttttgt
     1621 aaatcgacgc caggagcgat ttcgattaaa tgcaggccgt cttctttcag cgtgaatacg
     1681 gcgcgctcag tgatataacg aacatccagc cctcgctcga gcgcgatttt tccgctaaaa
     1741 gtaatttctg gtagttcccg aataaatttc ttcacccgtc cttcctggac gatatttaat
     1801 ttgccgtcgg taatttctgt ttttaaactg cccgcagtta atgtgccgca gaaaacgatt
     1861 ttcttcgaag tggcactgat atcaataaat ccaccggtgc ccatgatttt accattgaat
     1921 ttatgcacgc cgacgttacc gtgctggtcg acttcagcaa aactcaaata acaaacatcc
     1981 agaccaccac cgtgataaaa atcaaactgg gacgtcatat ccagaatggc gcgggtattc
     2041 acgttcgcgc caaaggcgat cccctgtgaa gtaataccgc caatcggacc tgtttctacc
     2101 gtcagaataa agtcatcagc acaaccttct tcccgggcga ccaggccaat gccgtcagca
     2161 ataccgacgc cgacattccc cactgcgcct ttgcgcattt cgaataatgc acgccgtgca
     2221 actaatttac gttggtttag gggcaggcta agtttggtgc tgtcatcaag ggtgaagtca
     2281 ccagaaataa agcggttaac cggcgctccg ccatacagtt gggtttgatc cggatcgacc
     2341 accacaatat ccaccagata acccggaata cggacagatt taggatgcag cgtggctttc
     2401 ttaaccattt tctgcacctg catcatcaca ataccgccgt tattgtgcac cgcctgagca
     2461 ataaccaatg cgtcgagata catcacctca tcttcaaaag tggcgtagcc ttcgctgtcg
     2521 caggtggtag cgcgaatgaa ggcgatatct ggcgctatcg ctttgtaata gagatattct
     2581 ttgttatcaa actcgaccag tttaatcagg tcttctttag tgacttcatt cagtttgccg
     2641 ccttgctggc gtggatcgac aaatgttccg atgccaatat cactaataat accaggctgg
     2701 tgggctgcgg cggcgcgtaa ggtttgtgta agtacacctt gtggatagtt ataagcaata
     2761 attttatttt gttctgcgag atcagaaata cgcggcgatt gtccccagtg accgcataat
     2821 gcccatttca ccaggccttc ttgcgccagc ggactaatac cacggtcggc gcgatcgcca
     2881 agccctgttg gactaataat cgataaatta cgtggtgttt gagtctgttt atatttatca
     2941 gcaagagcag taattaacgt ggtggcttcc agaataccgc cgccagcgcc taacacacaa
     3001 agtgttgctt cgtcgggaat ataattcacc gcttcctgtg ccgacaggac cggcactcgt
     3061 ccattaatgc gaggtggttt tacaggtttc attgttaatt cctgattcag gtgtgatttt
     3121 ccctacgcca gatggagaat tagcgcacag agactcacga aatattttta ttaaatacgg
     3181 ttaaaaatac tttttttagt tcggcggggg agagtattcc cgccgaaata ttattgctta
     3241 tgcctgatgt aaaatagtta ataccgtacg caaatcattt accgagattt gccctggcgc
     3301 agaggctttt tttaccgcac caaaagttgc cgccgagcca aatacttcac cagccagacg
     3361 agaaattacg ccagtttttg ccatcgacat cgtgatgatt ggacgatcgg catactgctc
     3421 ctgcatctcc agggtcgcag caagcaacgt cagcacatcg ctggtacttt gcggcatcag
     3481 cgcaatctta ggaatatcgg catcgaagga ctgcattttg cgcagacggg caatgatttc
     3541 ttcggcttcc ggcgttttat ggaagtcatg gttggacatg acaactttca catcatgcgc
     3601 gtgggcgtag gcgacggttt ctttaacctg atcatcgccg gtaaataact ccagatcgat
     3661 tatatcaacc aggccgctgt cgatggctgc acgattgagt gcaatataag cctcggtgga
     3721 aatcgcctgc tcgccgcctt ctttggcact gcggaaggta aacagcagcg gtttttctgg
     3781 catggtttca cggagaattt ttgctgccgc tatgacagac tccacattgg agagatcggc
     3841 atagtggtcc acacgccatt ccagaatatc aaagtccgct tcacgatagg cgagagcttc
     3901 ggatttcacg ctggcgatat ctttcgccat cagcgagacg atgattttag gtgcgcccgt
     3961 accaatgacg agatctttta cagttacggt tttcattttt taccctttct gcacgcggtc
     4021 agcctgtcag gcaccgaacc ccatgacctg tttaacatat tccagaggga aatctttgcc
     4081 agtccataac gtgaactgtt cagccccttg ccacaacaac atgccgtatc catcaatcgt
     4141 tttgcaaccc gcttgttgcg cctgctgcaa taacttcgtc atatgagggt tatacacgca
     4201 ttcagtgacc agaagtcccg gatgtaacag actgatatca ttaaccaatg attcattctc
     4261 aaggggtttc atacccactt tggtgccatt ggttaaaatg tcggcggaag ccagggcttc
     4321 tgcaaaggct tgctgatcgg cgagatcggt gactgtcacg acgcaatcgg tgttttcgtt
     4381 aacccgctgc gcgaaggcga gagctttatc gaagaactca tcccgacggt taaagagttt
     4441 aatttctttt aaaccttcaa ttgccccctg cgcgccaatt gccgttgagg caccaccggc
     4501 ccctaatagc accatcgttt tgcctttgat atcaaaaccg ctctctttaa tggcgcgaat
     4561 atgacccgtg ccgtcggtgt tatagccacg cagatagcca tcatcattaa cgatggtgtt
     4621 gatggctccc accagtttgg ccgctggcgt taattcatca acatattcac acgccagttg
     4681 tttgttcggc attgatacac cagttccgcg cattttgagg gcttttaatc cttcaattgc
     4741 tccgggaaaa ctatcgttat ccacttcaaa ggccatatag gtaaatggca atcccgcttt
     4801 ttctaaggct ttattctgca tttcgggcga taaactgtgg cggatgggat aggccatcaa
     4861 cccaatcaat tcgtattttg cggtaacatc cataattaac tcctttaacc tctatgctta
     4921 atcgtgttta accgggtgct aaatttatgc tcgccaaacc gcacatcatt ttcaggaata
     4981 atgaaaacgc ggtaatagcg gataaaaaca ataattgcgg taatcagggc cagcagcgcg
     5041 aaagtaaaat cgagaacaat gatatattgc aggccgatgt tcgacagata accggtaatc
     5101 agcggaataa caaagttagc cagtccaccc atcatcatat atatactggt gactttggct
     5161 ttgctttttg ggaaaaactc tgacatgacc gaaacgccga gctgtaaaat gccgccagct
     5221 gctgagaaac cgataacaaa ggctccggca ttacacacca atggagaagg gtacaggtag
     5281 ataatggcag ctgttattgt tgccagtgca gagttaaata cattagccca gatgggccgg
     5341 accatttttt tcagcagggc ggcaaaaata aagacacaga ccagcgaacc catactgtaa
     5401 taagagatgg tttttaatgc ctcagcttct gacataccag caaaagccat cgcatatttg
     5461 ggcatccaca ccacaatcac ataaaaggtc gcgaatgcgg ctacaccgaa cagtactgat
     5521 gaaacacctt ccagccagac taacggtttg ctgttcattt gcggtaattc attagctacg
     5581 ctggcgtcca ccaactggct ggggaatttg cttttcaaca gcatcagcgt gatcagtaca
     5641 aacagaatac ccggaataat cagcccatag ccgtaccaga tgttattgag caacatatag
     5701 ctcaccagca ttgggtagaa catttgcccg aatgacacca tcgctttaac cagtatgacc
     5761 gccgaaccag aggctttcgg aaagcattcc ataagcgcgg ggtagccacc cgtatccagc
     5821 gctgagttag cgatacctac gcacactgcc agacagtagg cgagagttaa attcgggcaa
     5881 gcaggaatac caaagaagaa tagcagatac attattactg ccattaatat caccgcccga
     5941 cgaccaaact tatcggagat cacaccgaag aataaaatac tgatcaatcg ccccaatccg
     6001 ataccggaaa ttaaataggc aatgcccgcg ttgtcagtgg aaaacttttc tgccaaagat
     6061 gtcatgtttt gggcaagcgt aataacacta atgccgtgca ggaaatagct gaagtaaata
     6121 caaagaacag ccaggataaa tggcgtgctg aaagccttat tttgagacat tttcacagct
     6181 ccttgctgaa agataatttc gttaccgtac gtattgttct ccgacggttt acataattac
     6241 tatactgaag ataattttcc ctgggtacat ggcataaagt ttatgtttcc aggaatattg
     6301 catgtcatag tttgtttttg atcgcgattt tccttagtta gcgcagaaag agttatttat
     6361 ccgtaagatc ttgcatgacc tgaaaagaat attgcattac ccacccggag cgacattttc
     6421 ctttagcgag tgatgccgcg tttctttgcg actgcgtaag ccaataaaca gtgccagtat
     6481 aaagccgatg gcagcgatgg cggtatcgaa ccacataata tcggcaatac ttctttgcga
     6541 aagatgagcc gtaatcaacg gaatagtaaa ggttgcaatg ctgcccgcac tgtaatagat
     6601 ccccgttgct tttcctttag cgtagggaaa acgttccgcc attaacgtca ggccaatttg
     6661 tacaacaccg cctgcggagg taaaaccgat gacgaaagca aatattatca ccacataaaa
     6721 tgtgggatgc aggcagacgg tgagcagggc gataaatgag ataaaggtgt acagcatcag
     6781 taatgttgtt gggcgaacgg tattacgaat gagtggagcg gtaataaata cacaaagcag
     6841 cgaacccacg gtgtagatac tgagtaattt gatcgacatg gtgtatgaca tgccggcgac
     6901 aaattgtccg tattgtgcca gccactggct aaccagataa aacgttgcca ttgagatata
     6961 gccatatagg gaataactgg ctaaatcgat aattgaacag cgatgttccg tagagctggt
     7021 ggttttcttt atgacaggta agtgacgacc cggatggggt gggaacgtac aacggtataa
     7081 aagcagagcg ttaataaaca taatgcctgc ggcaatcata aaggaccaac cgaaccacag
     7141 ttcagcccac actaacaggc tgataattag cggtaataaa aattgtccgc tggaaacaaa
     7201 tgctttaatt aaaatattgg ctgtacccgg tgagcgtgga aaagcttcca tcaaactggg
     7261 ataggtgcct gcatcgagaa aactgtttgc cattcccgcc agaaagccaa aaacataagc
     7321 gataatgatg ttattggtgt gcaggatgcc aaaaaagaag gccatatagc agcacatccc
     7381 gagcatgata aaagggcggc gaccaaagcg atcggataat aatcctgcaa aaagcaagac
     7441 acttaatcga ccaatgccca gtgatgagat aactatcgag acgcccgcgg cattagtctg
     7501 ccaaagcgtc tccagcgagg ccatattcag gctcatcaaa atgacgccca taccatgcac
     7561 caggtaatta aaatacaacc caagtgcggt agggtaatag ggatttttca taggttctac
     7621 ttagcaaagg tgctttaaaa agtgtagaga tgttgtgtca gtatccgttc attattgttg
     7681 taacaaagac aattcctttg tgtggagaaa ttaacaaagc cgctccagat cgtgtgcata
     7741 taattcagcg gctaccgatt gatagatctt ccagtcaata aaattgccat cagggtagac
     7801 ttgttgaaac acggttaagt cggggaaaaa acgcatggtg ttgttgccga tgcggttatt
     7861 tattttctct atgatggcat gaagatgttt ttttcgttgc tgacgcatag ccaacagttg
     7921 ctcgactaca cgatcaggaa tggcgcattt gccattttcc cactgacgcc agctttcact
     7981 gtcatcggtc tgggcaatcc aggtcgcgca ttcatcaata gtcatggcaa aaatatggcg
     8041 aagtgcctgg agttcataag cgttcatcgt gtaatggctg catgaaataa acaataaaat
     8101 ataaataaca ttatcagcct gctgacggct tgaagagagt cacagtatct tgtgcaacat
     8161 tattggaaag cgtgaggggc gagatagcaa agcggaaaaa tgttaaaaaa aagcccatcg
     8221 tgggagatgg gcaaagacta cacacagcaa ttcgttgttt cactcagggg atttccatgc
     8281 ttataaatca atatgttgat ttataaccgt gagctaatag taggcatacg gactttttgc
     8341 gtcgatcaga ttcgtctcat tagtttaaaa actgtaaaga atttgcgaca ggtactacca
     8401 gctgaatggc ggtaagcagg aaaatatggg gaagagcaag caaatatcaa ttagcagggg
     8461 ttaacaatca ggcggcagag ccgcctgatg agaaattact tattcgattt ctcgatttcg
     8521 ccaagttctt cgagaatttc ttccggttgg tccgtcggcg gtgggacttc ttccacccac
     8581 gcggcaaaaa gacgccagga aacggctaac agaaccggac caataaacag gccgatcatc
     8641 ccgaaagcaa tcaaaccacc aataacacca gagaggatca ggatcagcgg taaatcggca
     8701 cccatgcgaa ttaacattgg gcggatgacg ttatccagcg tgccaaccac accgctccac
     8761 actaacaata ccgttcccca ggtggtatca ccggtccagt agagccagat aatcgccgga
     8821 atcagtaccg gcaacggtcc aagctggaca aggcaggaga ggatcattaa caccgtgagc
     8881 aaagttgcat aaggtacgcc ggatacggcg aggccgatac cgccaagcac tgcctgtact
     8941 aacgccgtca ccaccacacc cagcgccacc gcgcggatag cctgtgccgc cagtagcaca
     9001 gcagcatcac cgcgaacgcc tgccagacgg gttgcaaaat ggcgaatgcc ttgtgccacc
     9061 tgttcaccgc gccagtacag cagggcactg aagagaagca tcagcgcaca atgcaccata
     9121 aagcgcccga tatgcgccgc ctgcccaacg aaccaggtgg tggtggtgcc aatataagga
     9181 cggactttcg ccatgatcgc cgtacccccc atatccagca agttgtgcca gcctgcatat
     9241 agcttcgcgc caatcaccgg aatggtatta agccacgcca gatcgggtaa cgtcatgtca
     9301 ccgctggaaa tggctttaat tagcggaccg ctgccatcga caatactgtt aaccaacaag
     9361 gcaataggga tgataaacac catcactaat aacagcgtca tcaccagaac ggcgagagag
     9421 cggcggccaa acatgatctt ttgcaaacgt aacaataccg gccaggtggc gataaccact
     9481 gtaccggccc atgcaaagcc gagaataaag ggttgaacaa tccacagaca tgccacaatc
     9541 atgatggcta aaaacagcac cgaaagcaga atttgtgcga catccctggg ctgacgaaca
     9601 tttaccataa ctacttttca ccttttttgt gcgtcaaatc gtcgacgcga cgtgaacacg
     9661 cgaaactcac ctgcataatg atgagcaatt tcagcggttt ttaacaggtc gattctgccc
     9721 ttaattctga tgggcgtgta agaaaaaaat gtggtacaac aactcaggca acacgcaaac
     9781 gatttactcg tcgtatttca aattgcatgt gctgcggcta cgttcgttca ccccagtcac
     9841 ttacttttgt aagctcctgg agttcactcg cttgccgcct tcctgcaact cgaaatccat
     9901 agagtaaaca aacatcacct ttcagtgcgc tattagcagc ctgaaagaac agtgtatcga
     9961 tctacatcac agaccgcagg aaagggtcaa tataatgatt ccacagattt cccaggcacc
    10021 cggcgtcgtt caattggtgc ttaatttttt gcaagagctg gagcaacagg gttttactgg
    10081 cgatacggcg acaagttatg ccgatcgtct gacaatgtcg accgacaaca gtatttacca
    10141 acttctcccc gatgcggtgg tatttccgcg ttcaaccgca gatgtggcgc tgatcgcccg
    10201 tcttgccgcg caggaacgct attcatcgct gatctttacc ccccgcggcg gcggcaccgg
    10261 cactaacggt caggcgctca accaggggat tattgttgat atgtcccgcc atatgaaccg
    10321 catcatcgaa attaaccctg aagagggctg ggtgcgcgtt gaggccgggg tgataaaaga
    10381 tcaactcaat cagtacctga aaccgttcgg ctactttttt gcgccggaac tttcgaccag
    10441 caaccgggca acgctcggtg ggatgatcaa taccgatgca tccggtcagg gatcgctggt
    10501 ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg gtgttgttgg ggggcgatat
    10561 tctcgatacg caacctttac ccgtcgaact ggcggaaacg ctgggtaaat ccaataccac
    10621 aatcgggcga atttataaca cggtttatca acgttgccgt cagcaacgcc agttaatcat
    10681 cgacaacttc cccaagctta accgctttct taccggttac gatctgcgtc atgtctttaa
    10741 cgatgagatg accgagttcg acctgacgcg cattctgacg ggttcagaag ggacgctggc
    10801 ctttattacc gaagcgcgac tggatattac gcgcttgcct aaagtgcgcc gtctggtgaa
    10861 cgtcaaatat gactcttttg actccgcact gcgtaacgcg ccgtttatgg ttgaggcgcg
    10921 ggcgctttcg gtagagacgg tggactcaaa agtgctgaat ctggcgcggg aagatattgt
    10981 ctggcattcc gtcagcgagt tgattaccga tgtgcctgac aaagaaatgc tcgggctgaa
    11041 cattgtggaa tttgctggtg atgatgagac gctgattgat gagcgggtaa atgcactctg
    11101 tgcgcggttg gatgaactga ttgccagcca acaggcaggt gtgattggct ggcaggtgtg
    11161 ccgcgagctg gcgggtgttg aacgtatcta tgcgatgcgc aaaaaagccg ttggtctgct
    11221 tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct gaggatacct gcgtaccgcc
    11281 ggaacatctg gccgattata ttgctgaatt tcgcgcgctg ctcgacagcc acggcttaag
    11341 ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac gtccgtcctg cgctggatat
    11401 gtgcgatccg caacaagaga ttttgatgaa gcaaatctct gatgacgtag tggcgcttac
    11461 cgcgaaatac ggtggtttgt tgtggggcga acacggcaaa ggttttcgtg ccgaatacag
    11521 ccctgcgttt ttcggtgagg aactttttgc agaactgcgc aaagtgaaag cggcatttga
    11581 cccgcataac cgactcaacc cagggaagat ttgcccgcca gaaggtctcg atgcgccgat
    11641 gatgaaagtg gacgcggtga agcgcggtac attcgatcgg cagatcccca ttgcggtacg
    11701 ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt ttatgcttca actttgatgc
    11761 ccgtagttcg atgtgtccgt cgatgaagat cacccagaac cggattcatt caccgaaagg
    11821 gcgcgcaacg ctggtgcgtg aatggctgcg cttgttggcg gatcgcggcg ttgacccact
    11881 caaactggaa caagaactgc ctgaatccgg cgtcagtttg cggacgttaa ttgcccgcac
    11941 gcgcaatagc tggcatgcga ataaaggcga atacgacttc tcacacgaag tcaaagaggc
    12001 gatgtcgggc tgtcttgcct gtaaagcgtg ttcgacccag tgccccatca aaattgatgt
    12061 gccggagttt cgctctcgtt ttctgcagct ctatcacacc cgttatttac gcccgcttcg
    12121 tgaccacctc gttgctacgg tcgagagcta cgcaccgctg atggcacgcg cgccgaaaac
    12181 ctttaacttc ttcattaacc agccgctggt gcgcaagctc tcggaaaaac atatcggcat
    12241 ggttgatttg ccactgctgt cagtcccctc gctacaacaa caaatggtgg ggcatcgctc
    12301 ggcgaatatg acgctggaac agcttgaagc tctcaatgca gagcagaaag cgcgcacggt
    12361 gttggtggtg caggacccct ttaccagcta ttacgatgcg caagtggtgg cggattttgt
    12421 ccgtctggtt gaaaaattag gtttccagcc tgtgttactg ccattttcgc caaatggcaa
    12481 agcccagcat atcaaaggct ttcttaatcg ttttgcgaag acggcgaaaa agacggcgga
    12541 tttcctcaac cgtatggcga agctggggat gccaatggtg ggcgtcgatc cggcgctggt
    12601 actttgttat cgcgatgaat ataaactggc gctgggcgag gaacgtggcg agtttaacgt
    12661 cttactggcg aatgaatggc tggcaagcgc acttgactca cagccagtgg ctacagtcag
    12721 cggtgaatca tggtatttct ttggtcactg taccgaagtt accgctttgc cgggcgcgcc
    12781 agcacaatgg gttgcgatat ttgcccgttt tggcgcgaaa ctggaaaatg tcagcgtggg
    12841 gtgctgcggc atggcaggga cttacggaca tgaagcgaaa aaccataaaa attcgctcgg
    12901 gatctatgag ttatcctggc atcaggcgat gcagcgactg ccgcgtaatc gctgtctggc
    12961 gaccggatat tcctgccgta gccaggtaaa acgggtcgaa ggcacggggg tacgccatcc
    13021 tgtgcaggct ttactggaga ttattaaatg atatggaaac gaaaaatcac cctggaagca
    13081 ctgaatgcca tgggtgaagg aaacatggtg gggttgctgg atattcgctt tgaacatatt
    13141 ggtgatgaca cccttgaagc gacaatgcca gtagactcgc ggacaaagca gcctttcggg
    13201 ttgctgcatg gaggggcatc cgtggtactg gccgaaagta tcggttccgt tgccggttat
    13261 ttatgtaccg aaggtgagca aaaggtggtt ggtctggaaa tcaatgctaa ccacgtccgc
    13321 tcggcacgag aagggcgggt gcgcggcgta tgcaaaccgt tgcatctcgg ttcgcgtcac
    13381 caggtctggc agattgaaat cttcgatgag aaagggcgtt tgtgctgttc gtcacgatta
    13441 acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga cgcatattgc ttttggtcat
    13501 tatctgcaca gtaaacggtg gtatactgtg caggtcttgt ctaaaagcga ggatgctatg
    13561 tctactcaac ttgatcccac ccagctggcc attgaatttt tacgccgtga ccagagcaat
    13621 ctttcacctg cccaatatct caaacgtctg aaacaactag aacttgaatt tgctgacctg
    13681 cttactctct cttctgctga attaaaagaa gaaatctact ttgcctggcg tctgggcgtg
    13741 cattgatcct gacttaatgc cgcaattttg ccaaatcgac ctggattaac ctccgtccag
    13801 gttcgatgac cttcttaata ctgcttatct atccgcctga tattgttcaa aaataatact
    13861 aatcgcagtt tgtgttaaaa cggcgggtta gctttatgag attattctta tcgccccttc
    13921 aagagctaag ccactgtgag tgccggagat aagcgccgga tggggtagaa acccttaagc
    13981 ctgtgtcgca cagacttaag ggttttctta tttctgaacg ctgcactttt ttacctatcc
    14041 cttaaatagt cattttcaat acaacttttt ctttttcatc acagccttaa tgctccttag
    14101 ataataatca ttatctaaca atgagatacc taattcttag cgtgcctgtt aacccacaca
    14161 tcagggtcta tgcttattaa aagcaggtaa aaagcatgtt ttacgctaaa gtccctgcgt
    14221 ttgctgggtt gaactgataa tcattatcac taacatgctg ttatacgctg aaagcgatga
    14281 agtgaggtaa atcgatggac atgcattcag gaacctttaa tccacaggat ttcgcctggc
    14341 agggcttaac gctgacaccc gcagccgctg tccacatccg cgagctggtg gcaaagcagc
    14401 cgggtatggt cggcgtgcgc ttaggcgtga agcaaacggg ctgcgcgggc tttggctatg
    14461 tgctcgacag tgttagcgag ccggacaaag acgatctgct gtttgaacac gacggcgcga
    14521 agctgtttgt cccgctgcaa gcgatgccgt ttattgatgg cacggaagtc gatttcgttc
    14581 gtgaaggact taatcagata ttcaaatttc acaaccctaa agcccagaat gaatgtggct
    14641 gtggcgaaag ctttggggta taggcggtac tatgtctcgt aatactgaag caactgacga
    14701 tgtcaaaacc tggaccggcg gcccgctgaa ttataaagaa ggattcttca cccagttagc
    14761 caccgatgag ctggcaaagg ggataaacga agaggtggtg cgcgcaattt cggcgaagcg
    14821 taatgagccg gagtggatgc tggagtttcg tctaaatgcc tatcgcgcat ggctggagat
    14881 ggaagaaccg cactggttga aagcgcacta cgacaagctg aattatcagg attacagcta
    14941 ctattcggca ccatcgtgcg gtaattgtga cgacacttgt gcgtctgaac ccggcgcggt
    15001 gcagcaaact ggcgcgaacg cctttttaag taaagaggtg gaggcggcgt ttgagcagtt
    15061 gggcgttccc gtgcgggaag gcaaagaggt ggcagtggat gccattttcg actctgtttc
    15121 ggttgccact acttatcgcg aaaaactggc ggagcaggga attatttttt gttcctttgg
    15181 cgaggcgatc cacgatcacc cggaactggt gcgtaaatat ctcggcaccg tggtgccggg
    15241 gaatgacaac ttctttgccg cacttaatgc ggcggtagcc tctgacggta cgtttattta
    15301 tgtgcctaaa ggcgtgcgtt gcccgatgga actttccacc tattttcgca ttaacgcgga
    15361 aaaaaccggg cagtttgagc gcaccattct ggtggccgac gaagacagct acgttagcta
    15421 cattgaaggc tgttccgctc cggtgcgtga cagctatcag ttacacgcgg cggtggtgga
    15481 agtcatcatc cataaaaacg ccgaggtgaa atattccacg gtacaaaact ggttccctgg
    15541 cgataacaac accggcggta ttctcaactt cgtcaccaag cgtgcgttgt gcgaaggcga
    15601 aaacagcaaa atgtcatgga cgcaatcaga aaccgggtca gcgattacgt ggaaatatcc
    15661 cagctgcatt ttgcgcggcg ataactccat tggtgagttt tactcagtgg cgctgaccag
    15721 cggtcatcag caagcggata ccggcaccaa gatgatccac atcggtaaaa acaccaaatc
    15781 gaccattatc tcgaaaggga tctctgccgg acatagtcag aacagttatc gcggcttagt
    15841 gaaaatcatg ccgacggcaa ccaatgcgcg caatttcact cagtgcgatt caatgctgat
    15901 tggcgctaat tgtggggcgc ataccttccc gtatgtcgaa tgtcgcaata acagcgctca
    15961 gctggagcac gaggcaacga catcacgtat tggtgaagat caactgtttt actgcctgca
    16021 acgcgggatc agcgaagaag atgccatctc gatgattgtt aacggttttt gcaaagacgt
    16081 gttctcggaa ctgccgctgg aatttgccgt tgaagcacaa aaactcctcg ccatcagtct
    16141 tgaacacagc gtcggataag gaataaacat gttaagtatt aaagatttac acgtcagcgt
    16201 ggaagataaa gctatcctgc gtggattaag cctcgacgtt cgtcccggcg aagttcacgc
    16261 cattatgggg ccaaacggtt ctggcaaaag taccttatcg gcaacgcttg ccgggcgaga
    16321 agattatgag gtgacgggcg gcacggttga gttcaaaggc aaagatttgc ttgcgctgtc
    16381 accggaagat cgtgcaggtg aaggcatctt tatggccttc cagtatccgg tggaaattcc
    16441 cggcgtcagt aaccagtttt tcctgcaaac ggcacttaat gcggtgcgca gctatcgcgg
    16501 ccaggaaacg ctcgaccgct ttgattttca ggatttgatg gaagagaaaa tcgctctcct
    16561 gaagatgccg gaagatttat taacccgttc ggtaaacgtt ggtttttccg gcggtgagaa
    16621 aaagcgcaac gatattttgc aaatggcggt gctggaaccg gagttatgca ttcttgatga
    16681 gtcagactcc gggctggata ttgacgcatt aaaagtggtt gccgatggcg tgaactcgct
    16741 gcgtgacggc aagcgttcat tcatcattgt tacgcactac caacgcattc tcgactacat
    16801 caagcctgat tacgtccatg tgctgtatca gggacgaatt gtgaaatccg gcgatttcac
    16861 gttggtcaaa caactggagg agcagggtta tggctggctt accgaacagc agtaacgcac
    16921 tgcaacagtg gcatcatttg tttgaagctg aaggggcgaa acgctccccg caagcacagc
    16981 agcatttaca acaattgctg cgtaccggac tgccgacacg taaacatgaa aactggaaat
    17041 atacgccgct ggacgggctg accaatagcc agtttgtcag cattgcgggg gagatatccc
    17101 cacagcagcg tgatgcctta gcgttaacat tagacgccgt gcggctggtg tttgtcgatg
    17161 gacgttacgt gcccgcactg agcgatgcaa ctgaaggcag cggatatgaa gtgagcatta
    17221 acgacgaccg tcaggggtta cccgatgcta ttcaggcgga agtgtttctg catttgacgg
    17281 aaagcctggc acaaagcgtg acgcatatcg ccgtgaagcg cggtcaacgg ccggcaaagc
    17341 cattgctgtt aatgcatatc acccagggcg tggcaggtga agaggtgaat actgcccatt
    17401 accggcatca tctggatctg gcggaaggtg ccgaagcaac ggtgatcgaa cattttgtca
    17461 gccttaatga tgctcgtcac ttcaccggcg cacggttcac tatcaacgtc gcagcgaacg
    17521 cccatttgca gcatatcaag ctggcgtttg aaaacccgct cagtcaccac tttgctcata
    17581 acgatttgtt gctggctgac gatgccaccg catttagcca cagtttcctg ctgggtggcg
    17641 cagtattacg acacaacacc agtacgcaac tcaatggcga aaacagcacg ctgcggatca
    17701 atagcctggc gatgccggtg aaaaacgagg tgtgtgatac tcgtacctgg ttggaacaca
    17761 ataaaggttt ttgtaacagc cgacagttgc acaaaactat cgtcagcgac aaaggccgcg
    17821 cggtatttaa cggtttgatc aacgtcgcgc agcacgccat caaaacggat ggtcagatga
    17881 ccaacaataa tctgctgatg ggcaaactgg cggaagtgga tacgaaaccg cagctggaaa
    17941 tctatgcaga tgatgtgaaa tgcagccacg gcgcgacggt ggggcgtatt gatgacgaac
    18001 agatgtttta tctgcgctcg cgcgggatca atcagcaaga tgcccagcag atgatcattt
    18061 acgcctttgc tgccgaactg acggaagcac tgcgtgatga ggggcttaaa cagcaggtgc
    18121 tggcccgaat cggtcaacga ctgccaggag gtgcaagatg actttttccg tcgacaaagt
    18181 gcgggccgac tttccggtgc tttctcgtga ggtaaacggt ttgccgctgg cttatctcga
    18241 cagcgccgcc agtgcgcaga aaccgagcca ggtgattgat gccgaggctg agttttatcg
    18301 tcatggctac gcggcggtgc atcgcggtat tcatacctta agcgcccagg cgaccgagaa
    18361 aatggagaac gtgcgcaagc gggcatcgct gtttattaat gcccgttcgg cggaagagct
    18421 ggtgttcgtc cgcggcacga cggaagggat caatctggtc gccaatagct ggggcaacag
    18481 caatgtgcga gcgggcgata acatcatcat cagtcagatg gagcaccacg ctaacattgt
    18541 cccctggcag atgctttgtg cacgcgttgg cgcagagctg cgtgtgatcc cgctcaaccc
    18601 cgacggtact ctgcaactgg agacgctgcc tacgctgttt gatgagaaaa ctcgtctgct
    18661 ggcaattact catgtctcca acgtgcttgg gacagaaaat ccgctggcgg agatgatcac
    18721 acttgcgcat caacatggcg caaaagtgct ggtggatggc gctcaggcgg tgatgcatca
    18781 tccggtggat gttcaggcgc tggattgcga tttttacgtg ttctccgggc ataaactgta
    18841 tggccctacc ggaattggca ttctttatgt caaagaagcc ttgttgcagg agatgccgcc
    18901 gtgggaaggg ggcggttcta tgatcgccac cgtcagcctg agtgaaggca ctacctggac
    18961 caaagcacca tggcggtttg aagccggtac acccaatacc ggaggcatca ttggtcttgg
    19021 cgcggcgctg gagtatgttt cggcgctggg gcttaataac atagccgagt atgaacagaa
    19081 tctgatgcat tacgcgttat cacagttgga atctgtaccg gatctcactc tctatggccc
    19141 acaaaacagg cttggcgtta ttgcttttaa tctcggtaaa caccacgcct atgatgttgg
    19201 cagttttctc gataattacg gcattgctgt gcgtaccgga catcactgcg caatgccatt
    19261 aatggcctat tacaacgtcc ctgcgatgtg tcgggcatcg ctggccatgt ataacaccca
    19321 tgaagaagtg gatcgtctgg tgaccggcct gcaacgtatt caccgtctgc tgggataaca
    19381 gggaggcact atggctttat tgccggataa agaaaaattg ctgcgtaatt ttttacgctg
    19441 cgccaactgg gaagagaaat atctctacat tattgagttg ggtcagcgtc tgccagaatt
    19501 acgtgacgaa gacaaaagcc cgcaaaatag catccagggc tgccagagtc aggtgtggat
    19561 tgtgatgcgt caaaatgcgc agggaattat tgaattacag ggcgacagcg atgcggccat
    19621 tgtaaaaggg cttattgcgg tcgtctttat tctttacgat caaatgacgc cgcaggatat
    19681 tgtcaatttc gatgtgcgcc cgtggtttga aaaaatggcg ctcacccaac atctcacccc
    19741 atcacgctca caaggtctgg aagcgatgat tcgcgcaatt cgcgccaaag ccgctgcact
    19801 tagctaaact aaagggacag ctttcatccg gttggctcac attgccgggt ggtcaatgtt
    19861 tacactggcc tgcacaggag tgttttggtt tcaggtgaac ataagcgcca tccgacgttt
    19921 cagcgtgagt ctccggcaaa tacaggaggt ttacaatgaa atgcgcgtct ttgcttacac
    19981 tcacgcttat cggcgctttt agcgccatcc aggctgcctg ggcggttgat tatccgctac
    20041 caccaaccgg cagtcgactg gttgggcaaa atcaaacgta tacggtgcaa gaaggggata
    20101 aaaaccttca ggccatcgcc cgacgttttg atactgcggc aatgttgatc cttgaagcca
    20161 ataacactat cgccccggtg ccaaaacccg gtacgacgat aactattcct tcacaactat
    20221 tattacctga tgcgccgcgt caggggatta ttgtaaacct tgcagagctg cgcctttata
    20281 attatccgcc gggagaaaat atcgtgcagg tttatccgat aggtattggt ttgcaggggc
    20341 tggaaacgcc ggtgatggaa acgcgtgtgg ggcagaaaat ccctaaccca acctggacgc
    20401 ctacggcggg cattcgtcaa cgttcgctgg aacgtggcat taaattacca ccagtcgttc
    20461 ctgccgggcc caataaccca ctaggacgtt acgcactgcg cctcgcgcat ggtaatggcg
    20521 aatacctcat tcacggcacc agtgcgccgg acagcgtcgg tttgcgcgtc agttcagggt
    20581 gtattcgcat gaatgcaccg gatattaaag ccttattctc cagcgtgcgg acgggaacgc
    20641 cggtgaaagt gatcaacgaa ccggtgaaat attccgtgga gcctaacggg atgcgttatg
    20701 ttgaagtaca tcgaccacta tcggcagaag aacagcagaa cgttcagaca atgccataca
    20761 cactgccagc aggctttacg caatttaaag acaataaggg tgtagatcag aaattagtcg
    20821 ataaagcgtt gtatcgtcgg gcagggtatc cggttgcggt gagcagtgga gcaactcccg
    20881 cagccagcaa tgcgccttca gtagagtcag cgcagaatgg tgaaccagag caagggaata
    20941 tgttacgcgc gacgcagtag cggtaaatgg caggcaaaaa aaatggcgca caatgtgcgc
    21001 catttttcac ttcacaggta ctattacttg cggtatttag tagccatgtt gtccagacgc
    21061 tggttagcac gagctgcgtc atctttagca gcctgaacgt cggaacgcat tgcgttcacg
    21121 tcgttgctca gctggtcaac tttagcgttc agagtctgaa cgtcagaaga cagctgatcg
    21181 attttagcgt tgctggagca acctgccagc agagtagaac ccaggattac cgcgcccagt
    21241 accagtttag tagctttcat tattaatacc ctctagattg agttaatctc catgtagcgt
    21301 tacaagtatt acacaaagtt ttttatgttg agaatatttt tttgatggga aggcacttat
    21361 ttttgatcgt tcgctcaaag aagcatcgaa cgctgggttt tagataaaaa aattgagaga
    21421 aaacaggatg cttccatcgg attcatctta gataaagtga gattatatag tgaaatccga
    21481 tttgattttt tattgcttct ggttatcgat taaataaaaa aagcgcccat cagggcgctt
    21541 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    21601 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    21661 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    21721 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    21781 ggtggtcagt gccaggatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    21841 accgccctgg gtagcaacca cgatcagcgg ggcatccagt ttttcagcag tttcaacggc
    21901 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    21961 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    22021 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    22081 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttaa tcatggaatc
    22141 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    22201 cttctgggcg aagataactt cttctaccgg gatttctaca cccaggtcgc cacgcgcaac
    22261 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    22321 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    22381 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    22441 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    22501 aggcaggttc acacctttgt tttcacccag gtcgccgttg ttcagcactt tacagataac
    22561 tttgttacct tcaatggcgg taacttccat accgatgaga ccatcgtcaa ccagtacggt
    22621 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    22681 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    22741 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    22801 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    22861 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    22921 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    22981 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    23041 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct cgctctaagg ataggtgaca
    23101 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    23161 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    23221 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    23281 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    23341 ttcgctgttt gcgcaacgtc tggtgccggg gttgtcactg tgaagataag tgaaagttca
    23401 tccggtgtat aaatacatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    23461 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    23521 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    23581 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    23641 tattgttatc gcaggcgtat tacgtacagc attagccaat aaacgcattc agcgcagtga
    23701 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    23761 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    23821 ataacaactg ctgaaat
//
LOCUS       DAZKJR010000079        23372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKJR010000079 DAZKJR010000000
VERSION     DAZKJR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.079"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1226
                     /locus_tag="ACHOWS_003074"
     CDS             <1..1226
                     /locus_tag="ACHOWS_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFQ0048617.1"
                     /translation="IPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAF
                     LSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNI
                     LDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKI
                     LVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIE
                     VSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
                     MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNS
                     AQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADQALR
                     EAKSEGGNKVIIHHI"
     gene            1407..2897
                     /gene="dgcJ"
                     /locus_tag="ACHOWS_003075"
     CDS             1407..2897
                     /gene="dgcJ"
                     /locus_tag="ACHOWS_003075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFQ0048618.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPDLRGTLQTASTDCDTIVEAAALLPAFDQAVESNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2940..3443
                     /gene="yeaK"
                     /locus_tag="ACHOWS_003076"
     CDS             2940..3443
                     /gene="yeaK"
                     /locus_tag="ACHOWS_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0048619.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3444..3548)
                     /gene="yoaI"
                     /locus_tag="ACHOWS_003077"
     CDS             complement(3444..3548)
                     /gene="yoaI"
                     /locus_tag="ACHOWS_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFQ0048620.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3718..4164
                     /gene="yeaL"
                     /locus_tag="ACHOWS_003078"
     CDS             3718..4164
                     /gene="yeaL"
                     /locus_tag="ACHOWS_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0048621.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4121..4942)
                     /gene="nimR"
                     /locus_tag="ACHOWS_003079"
     CDS             complement(4121..4942)
                     /gene="nimR"
                     /locus_tag="ACHOWS_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFQ0048622.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGVSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5039..6220
                     /gene="nimT"
                     /locus_tag="ACHOWS_003080"
     CDS             5039..6220
                     /gene="nimT"
                     /locus_tag="ACHOWS_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFQ0048623.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSTMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVAAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6275..6622
                     /gene="yeaO"
                     /locus_tag="ACHOWS_003081"
     CDS             6275..6622
                     /gene="yeaO"
                     /locus_tag="ACHOWS_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0048624.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6644..6898)
                     /gene="yoaF"
                     /locus_tag="ACHOWS_003082"
     CDS             complement(6644..6898)
                     /gene="yoaF"
                     /locus_tag="ACHOWS_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFQ0048625.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7081..8106
                     /gene="dgcP"
                     /locus_tag="ACHOWS_003083"
     CDS             7081..8106
                     /gene="dgcP"
                     /locus_tag="ACHOWS_003083"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFQ0048626.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8139..8237
                     /gene="yoaK"
                     /locus_tag="ACHOWS_003084"
     CDS             8139..8237
                     /gene="yoaK"
                     /locus_tag="ACHOWS_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFQ0048627.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8240..8314
                     /gene="yoaJ"
                     /locus_tag="ACHOWS_003085"
     CDS             8240..8314
                     /gene="yoaJ"
                     /locus_tag="ACHOWS_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFQ0048628.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8373..8621)
                     /gene="yeaQ"
                     /locus_tag="ACHOWS_003086"
     CDS             complement(8373..8621)
                     /gene="yeaQ"
                     /locus_tag="ACHOWS_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0048629.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8769..8951)
                     /gene="yoaG"
                     /locus_tag="ACHOWS_003087"
     CDS             complement(8769..8951)
                     /gene="yoaG"
                     /locus_tag="ACHOWS_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFQ0048630.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8955..9314)
                     /gene="yeaR"
                     /locus_tag="ACHOWS_003088"
     CDS             complement(8955..9314)
                     /gene="yeaR"
                     /locus_tag="ACHOWS_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFQ0048631.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9487..10125)
                     /gene="leuE"
                     /locus_tag="ACHOWS_003089"
     CDS             complement(9487..10125)
                     /gene="leuE"
                     /locus_tag="ACHOWS_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFQ0048632.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10252..11175)
                     /gene="dmlR"
                     /locus_tag="ACHOWS_003090"
     CDS             complement(10252..11175)
                     /gene="dmlR"
                     /locus_tag="ACHOWS_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFQ0048633.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11278..12363
                     /gene="dmlA"
                     /locus_tag="ACHOWS_003091"
     CDS             11278..12363
                     /gene="dmlA"
                     /locus_tag="ACHOWS_003091"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFQ0048634.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKKIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVID
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12614..14224
                     /gene="yeaV"
                     /locus_tag="ACHOWS_003092"
     CDS             12614..14224
                     /gene="yeaV"
                     /locus_tag="ACHOWS_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFQ0048635.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHHV
                     EHYLPQTPVEVLEKTPVLPAGTVFKGDN"
     gene            14256..15380
                     /gene="yeaW"
                     /locus_tag="ACHOWS_003093"
     CDS             14256..15380
                     /gene="yeaW"
                     /locus_tag="ACHOWS_003093"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFQ0048636.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15436..16401
                     /gene="yeaX"
                     /locus_tag="ACHOWS_003094"
     CDS             15436..16401
                     /gene="yeaX"
                     /locus_tag="ACHOWS_003094"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFQ0048637.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLNIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16448..17563)
                     /gene="rnd"
                     /locus_tag="ACHOWS_003095"
     CDS             complement(16448..17563)
                     /gene="rnd"
                     /locus_tag="ACHOWS_003095"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFQ0048638.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17645..19330)
                     /gene="fadD"
                     /locus_tag="ACHOWS_003096"
     CDS             complement(17645..19330)
                     /gene="fadD"
                     /locus_tag="ACHOWS_003096"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFQ0048639.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDSEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19535..20116)
                     /gene="yeaY"
                     /locus_tag="ACHOWS_003097"
     CDS             complement(19535..20116)
                     /gene="yeaY"
                     /locus_tag="ACHOWS_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFQ0048640.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(20155..20850)
                     /gene="tsaB"
                     /locus_tag="ACHOWS_003098"
     CDS             complement(20155..20850)
                     /gene="tsaB"
                     /locus_tag="ACHOWS_003098"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0048641.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGADLPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDEKGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLTLCDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20908..22818)
                     /gene="yoaA"
                     /locus_tag="ACHOWS_003099"
     CDS             complement(20908..22818)
                     /gene="yoaA"
                     /locus_tag="ACHOWS_003099"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0048642.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22950..23294
                     /gene="yoaB"
                     /locus_tag="ACHOWS_003100"
     CDS             22950..23294
                     /gene="yoaB"
                     /locus_tag="ACHOWS_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFQ0048643.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5739 a         5954 c         5679 g         6000 t
ORIGIN      
        1 tcatcccaca gatgaattac ctgatggttg ttgtggcttt gtttttcctt aacgccgtca
       61 ttttcctttt catgttaatg aagtatttca ctaacaaaca aattttacca acactcattt
      121 taagccttgc atttttaagt ggccttatct atttagttga aaccattgta attatccata
      181 aaccaatcaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct attttctata
      241 ttttccgcca actcagtttc atttgtttaa cctcgctggc gctcttttgt tatgggaaag
      301 acaacatcct tgacaacaat aagaagaaaa ctggcattct gttgctggcg ctgattcctt
      361 ttttagtttt tccccttctg gcacacaatc tgagcagtta taacgctgac tattctttgt
      421 atgtcgtcga ttactgtccg gacaaccata ctgcgacctg gggaatcaac tatacaaaaa
      481 tattggtttg tttgtgggca tttttactgt tctttattat catgcgtaca cgattagcca
      541 gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc
      601 tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg attgaagttt
      661 ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcaggaa ctgcaactct
      721 ccagcaaact ggcagttcat gatgtactga ccaatattta taatcggcgc tactttttca
      781 acagcgtaga gtcattattg tcgcgacctg ttgttaagga cttttgtgtc atgctggttg
      841 atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc
      901 tggtatcaat tgtcgatatt atccagcaaa gcatccgccc cgatgatatt ttagcgcgac
      961 tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca
     1021 ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac agatatgatg
     1081 ttcctgagca aatgaccatc agtattggca cggttttttc aacgggtgac acacgtaata
     1141 tctcgcttgt catgacggaa gcagatcaag ccttacgcga agcgaaaagc gaggggggca
     1201 acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc tttttgcgtg
     1261 gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga
     1321 tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt
     1381 cactaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc cggcatttta
     1441 tcgccgcaag tgtcattgtg ctgacatctt ccttccttat ttttgaactt gtcgccagcg
     1501 acagagcaat gagtgcctat ctgcgctata tcgtgcagag agcagactcc tcctttcttt
     1561 atgataagta tcagaatcag agtattgccg cgcatgtgat gcgcgctctc gctgctgagc
     1621 agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag tctgccaata
     1681 atacccatgg cttaaacctg actgcccata aatacccgga cttacgcggc acactacaaa
     1741 ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc gcttttgatc
     1801 aggcagtgga aagcaaccgc caccaggatg attacggttc aggtcttggg atggccgaag
     1861 agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat gaaccggtta
     1921 atgttgagta ctttgcgatg aataactggt ctttcctgca gtcaggaagt attggcatcg
     1981 atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt tttatcgagc atttaccagg
     2041 atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc gcagggcagc
     2101 taaaagggat tgtgctgctg gatattaaca aaaacaatct gcggaatatc ttttacactc
     2161 atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgatacc gattcggggc
     2221 gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt tacgtccatg
     2281 acttaccggg cggcattcgt gtctcgttat ccattgatat tctttacttt atcacgtctt
     2341 cgtggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat atggtgcgga
     2401 tgcacttccg tttataccaa aacgtgtcgc gagaaaatat tagtgatgcg atgactggac
     2461 tgtataatcg caaaatttta acccctgaac tggagcagcg gttgcagaag ctggtgcaat
     2521 ccggttcttc ggtgatgttt attgcaattg acatggacaa gttaaagcaa ataaatgaca
     2581 ccctcggtca tcaggagggg gatttagcga ttacgttatt agctcaggcg attaaacaat
     2641 cgattcgtaa aagtgattat gccatccgac tcggtggcga tgaattctgc atcattcttg
     2701 tcgattcgac gccgcaaatt gcagcacaac tgcctgaacg tatcgaaaaa cgtctgcaac
     2761 atatcgcgcc gcagaaagag atcggcttct cttccggtat ttacgcgatg aaagaaaacg
     2821 atacgttaca tgatgcgtat aaagcttccg atgagcgttt atatgtcaat aagcagaaca
     2881 aaaacagccg ttcatgataa ccttctgtgg ttgtttgctt gtaatctcag gagcatgaaa
     2941 tgactgaaat ggctaaagga agcgtgacgc atcagcggtt aatcgcttta ttatcacaag
     3001 aaggtgctga cttccgcgtc gttacccatg aagcggtagg taaatgtgaa gcggtgtcag
     3061 aaattcgtgg caccgcttta ggccaggggg ctaaggcact ggtatgtaaa gtcaaaggca
     3121 atggtgtaaa tcaacatgtt ctggcgatcc tcgcggccga tcaacaggcc gatctgagcc
     3181 aactcgccag ccatataggc ggattacgcg cttcgttggc cagcccggcg gaagtcgatg
     3241 aactcacagg ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca aaactcaaac
     3301 tggttgccga tcccctactc ttcgaacgat ttgatgaaat cgccttcaat gcaggcatgc
     3361 tggataaatc cgttattttg aaaaccgcgg attatttgcg cattgcgcaa ccagaactgg
     3421 tcaatttccg ccgcactgcg taactagcct gtccgttcga taagaagaac ggacaaaacc
     3481 agcacaacag caatggcaaa aaacgatgac gtgataatca gtgtctcgac gaacatttga
     3541 tcgttcatag tatgcccccg ggagatagtt gttttcacgt tattgttagc gtgcgcaaat
     3601 ggcagtttga tgacagttcg cctgattttt tatgcaaaaa acgtaaagat tttctacttc
     3661 cttcctgcag caagcgtaaa gtaagcaggc ttattattct ttggcaagga aaccacgatg
     3721 tttgatgtca ctttgctgat cctgctcggg ttagctgcgc tgggctttat cagtcataac
     3781 accactgtcg ccgtttcaat tctggtgtta atcattgtcc gcgtcacacc gttaagcacc
     3841 tttttcccgt ggattgaaaa acaggggcta agtatcggga taatcattct gacgattggc
     3901 gttatggctc ccatcgccag cgggacgcta ccgccctcaa ccctgattca ctcgtttctg
     3961 aactggaaat cgctggtggc aattgcagtc ggcgttattg tctcctggct gggggggcgc
     4021 ggcgtgacgt taatgggcag ccagccgcaa ctggtcgccg ggctgctggt gggcactgtt
     4081 ttgggcgtgg cgctgtttcg cggcgtaccg gttggcccgc ttattgccgc gggtctggtt
     4141 tcgctgattg tggggaaaca gtagttaatc ccgcaatata tcggcctggc gtttgcccca
     4201 ggcctttctt aaacatagta ataaaggccg ttgttgaatc ataccccagc gtatgagcaa
     4261 ctttctgtac agtatcgcct ttcactaatc cctgaagcgc cataatcagc tgtaactgct
     4321 gacgccactg gcgaaaactc acccccgtct ctttcactat cagtcgggca agattgcgtt
     4381 cactcatcgc aaaaaagcga gcccattgcc ccaatgaccc ccactcgacg ggctctttcg
     4441 ccatcatctc caccatcgcg cggattttag gatgagaaga aacgggtagg tgcagctgtt
     4501 gttgtggctg ctgagggagt tcatcaaaca ggacctgaat taatctgcgg gtcatcggct
     4561 cagcacgctg agtcgtcgtc ctgtttgcca gggttaaaat taattcccgg cataagggcg
     4621 atattttcaa tgtgcaacag gttgtcggca ttgtcactgc gctgggttcg ataaacagaa
     4681 aacagagttc agcgttagct gtcacttgat tgctatgttc cacccctccc ggtatccaaa
     4741 ccgcatattg tggcggcacc atccacaaag cattttccac cgtacaggta attgcaccat
     4801 gtagtgccag tatcaactgt cctttgcggt gctgatgtac aggactggtt aactcatccg
     4861 ttccggcgtg aatacaaaat gcgaccgcgg cttcatggtg tcggtcgggt tcatagccat
     4921 tgagattcaa cctgtgcatc attttgtccg aatttagcga taatttgtca ttttagcttg
     4981 attcaacata acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg agaagcatat
     5041 gacctgttca acttcattaa gcggcaaaaa caggattgtc cttatcgctg gcattctgat
     5101 gattgccaca acattacgcg tcacctttac cggcgcagca ccgttactgg atacgattcg
     5161 ttccgcttac tcgctgacga cggcgcaaac cggcttattg accaccctgc cattattagc
     5221 ctttgcgcta atctcacctt tggctgcccc ggtggcgcga cgttttggta tggaacgtag
     5281 cctgtttgcc gcgttacttt tgatctgtgc tggtatcgca attcgctctc tcccttcgcc
     5341 ttacttatta tttggcggta cagcggtcat tggcggtggg attgcattag gcaatgtctt
     5401 actgccagga ttaattaaac gcgatttccc tcattccgtc gccagactta ccggcgcata
     5461 ttccctgaca atgggagctg cagcggcact gggatcgact atggtcgtgc cgctggcttt
     5521 gaacggtttt ggctggcaag gcgcgttgct catgctgatg tcttttcctc tgctggctct
     5581 ttttttatgg ctgccacagt ggcgaagtca acaacatgca aatttgagta cctcacgcgc
     5641 cttacatact cggggtatct ggcgttcgcc gcttgcctgg caggtaacat tgtttcttgg
     5701 gatcaactca ctggtctatt acgtgattat tggctggctt ccggcgattc tcatcagtca
     5761 cggctatagc gaagcacagg cgggttcact gcatggtttg ctgcaactag ccacagcagc
     5821 tcccggtttg ctgatcccac ttttcttaca tcatgtgaaa gatcagcgtg gtattgcagc
     5881 gttcgttgcc ttgatgtgcg cagtggccgc ggttgggctc tgctttatgc cagcacacgc
     5941 gatcacctgg actctgcttt tcggttttgg ttccggcgca acaatgatac tggggttgac
     6001 gttcattggt ctgcgggcaa gttctgcgca tcaggcggcg gcactctcgg ggatggcaca
     6061 atccgtcggg tatttgctgg cagcctgtgg gccgccgctg atgggtaaaa tacacgatgc
     6121 taacggtgac tggtccgtac cactgatggg tgttgcaata ctttcactac tgatggcgat
     6181 tttcggactt tgcgccggga gagacaaaga aattcgctaa tatccggtgc tatagtgacg
     6241 tggcaaatca tgcgtgaaag ggagaacaaa tacgatgaat attcagtgca aacgcgttta
     6301 tgatccggct gaacagagcg atggttatcg cgtactggtc gaccgcctct ggccacgcgg
     6361 tatcaaaaaa accgatttag cccttgatga atgggataaa gaaatcacgc cgtcaacgga
     6421 actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca acctttcgcg agcaatatct
     6481 tgcagaactg gcgcaacacg agcaagaagg aaagcggctg gcgaacatcg ccaaaaaaca
     6541 gccgctgacc ctgctctact cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc
     6601 agactggcta cgtagcttgt gattttagta cgccatccgg cagttatttt tcaccagccg
     6661 gatggtcccg tcgccacaat gcccattcat caatcgtttc accgcccggt aatttgcaat
     6721 tgttgctgac cccttgcgct gtctgcacag gaatgagcgt cccgcccttc tgctggcaat
     6781 agaccgacgc cggatttgcc ataccaatct gcggcggttt aggtgcttct ggctgagaag
     6841 gggttgaaca accagccagg accagcaagc aaggcagaac aaaactgata attttcattt
     6901 attgatctca catatttatc cagacttaga gtatcgcggt atcgttttgt tttgcagcac
     6961 tatttttatt acattcactc aaatcatatt acgccttgtt tcatctttgt tgatgatgtt
     7021 ttatcatgcc tgcaaagatt aaataatcag catttacccg ccgtatcctg gagttgtttc
     7081 gtgtcagatc agattatcgc ccgcgtctcg caatcccttg ccaaagaaca gtcactggaa
     7141 agcctggtcc gacagcttct ggagatgctg gaaatggtca ctgatatgga atcaacctac
     7201 ctgaccaaag tggatgtcga agcgcgcctg cagcatataa tgtttgcccg taacagccag
     7261 aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt actcgttatg caaacgcgcc
     7321 attgatgaaa actgcttttt cagcgatgaa gtccctgacc gttggggcga ctgtattgcg
     7381 gcacgcaatc ttggcatcac cacatttctg agcacgccaa ttcacttacc ggatggatca
     7441 ttctatggca cgctttgcgc cgccagcagt gagaagcgcc agtggagtga acgcgcggaa
     7501 caggttttac agttattcgc cggactgatt gcacaatata ttcaaaaaga ggcactggtt
     7561 gagcagctgc gcgaagccaa tgctgcgctg attgcgcaat cgtataccga ctcgttaacc
     7621 gggctaccga atcggcgggc gatttttgaa aatctgacga cgctgttttc tctcgcccga
     7681 catcttaacc ataagataat gatcgcgttt atcgatctgg ataacttcaa attaatcaac
     7741 gatcgttttg gtcataatag tggtgatttg ttcctcattc aggttggcga gcgccttaat
     7801 acgctccagc aaaatggcga agttattggt cgtctcggcg gtgatgagtt tttggttgtt
     7861 tcactgaaca acgagaatgc ggatatttcg tctctgcgag aacgtattca acagcaaata
     7921 cgtggagaat atcacttagg tgacgttgat ttgtattatc ccggtgccag tcttggcata
     7981 gtagaagtcg atcctgaaac gaccgatgca gacagtgccc tgcatgctgc cgatatcgcg
     8041 atgtatcagg agaaaaaaca caaacagaaa acaccttttg tcgcgcatcc agcgctacat
     8101 tcctgaggcg tattcacatc cttttgattg gtgataacat gcgaatcggt attatttttc
     8161 cggttgtaat ctttattaca gcggtcgtat ttttagcatg gttttttatt ggcggctatg
     8221 ctgccccggg agcataaaga tgaaaaaaac aacgattatt atgatgggtg tggcgattat
     8281 tgtcgtactc ggcactgagc tgggatggtg gtaacgtcac atctagaaaa tagcaaaggc
     8341 tgcctgtgtg cagcctttgt gcaatttacg cgttaacttt taatctttct gtagataaac
     8401 agcacgacaa tcgcgccaat aacggcaacc acgaagctgc caaaattgaa gccatcgact
     8461 ttaccaaagc caaacagcgt gctgatccat ccgccgacta cggcaccgac tatccccagc
     8521 aggatagtca taaagaatcc accaccatct ttacctggca tgatccactt cgccagaata
     8581 ccggcaataa gcccaaaaat aatccatgac agaatgccca ttgtttcctc acttatctgt
     8641 tttgcattag cgggttagtc gctgataaaa agcatagcac aacatcgtga gggcaagatt
     8701 tgtgacgagc atcacggagg atttttttgc gatggcgcag aaattgcgcc atcaacgatc
     8761 agtgataatt accaaccaca aacatcatgt tcgttttccg tgtcataaga acgcacggta
     8821 ttcaccagat cttttatcac ttcagccgcc acttctggca ccagcaaagt catcggcgtc
     8881 tctgtttcat aatcgacaga aacgccattg ctgttattgg tgacggtcac ggtatacgtt
     8941 gctttgccca tgattcattt cccgttatga atgactttcc gttgttgcgc accttccatc
     9001 aggacttcag gagcaacgaa gaagtcaatg ttgaaataag tgtcgtcagt catggcttca
     9061 atgttgtgcc acttttctgg agggaacaca gcaaactgcc ccgcttcgat aaggatcact
     9121 tgatcaggct ctgtactgtg ttcatcagcg tagccgagat atttgaccgc cccatgcata
     9181 acggaaaggc gtgggtaaac ccccgggcgc gttcctttat caagatgacg ttcgaatatt
     9241 ccggcaggcg cagtttgttt attccagaaa ggcgttgagc gcgtatgaat ataattctgt
     9301 gggatttgca gcatcctttt ccctccttcg gtgaatgcgc tgaaaacggt ttatttcagc
     9361 cgtttcaggg tacgcctgat aatttgcatt ttaaatacca tttattggtt actatttagc
     9421 accgtatcag agaagaatca gggaggatta caggtgggaa gcccgcgaca acgcgggctg
     9481 aatgcatcag gactggagcg tcgccagtcg ggcggcgaaa cccacaaaca tcaaaccaat
     9541 cagtgagttg cccactttag ccagtttctt tttggtacgt atgtactgcg tgacaaaagc
     9601 accagaaata atcaggaagc tcaaatagca gaaactcacc agttccagcg tcgtcgccag
     9661 aataaagaat gaaattcccg tatgtggggc attaacatcg ataaactgta cgaaaaacga
     9721 cacatagaac aaaatggctt tcggattagt caggctcaaa attaacgcgc gtttaaaaat
     9781 ggcaccgtat tggggctcat cggatttggc ctcgttattt ttacccttca gggtcgcgta
     9841 gagaatttta ctccccagat agagcaaata aaacgcacca agataacgta caatgttgaa
     9901 taatatcggg gtggtcttaa ttaatgtcgc cactccagcc catgccagaa acatcaatac
     9961 cgcatcgcca ataaatacac cgcaggccgc aagataaccg cctttcatac cgctactgac
    10021 gctatttttg agtacaaaca gggtatttgg ccctggcacc aacacaataa aaatggcccc
    10081 aaccagatag gtccagtaat tcagaacccc gtattcagcg aacacattaa cctctttaat
    10141 tatctttcga tcatgcgcga ttaaaggtga atatgctaac caatctgtag cggcttagaa
    10201 aggagaaaat caggttttaa cctgatatca acccgataat tgaatcatta atcaggcatg
    10261 ctccagtgaa aaattcgggt agtgctcggc aaaatactgg cgtaaaaatt ctaccgttat
    10321 ccgtactttc gctgacgtcg ccagccttga aacataaacg gcccagacat tcgctggctg
    10381 gtaatattcc ggtagcactt gtactaaatg accactggca atgttttcgc taacatccca
    10441 ccaggagcgc agagcaatcc cctgcccatc gagacaccac tgatggacaa tttctccatg
    10501 attagacgac agtgggccgg taactttaat cgcatggtgg ccttctttat tgcgtaattg
    10561 ccagacacca aaaggatgat cgcgctcttt aatgaccagg cacggcaatg cagaaagatc
    10621 ggttaaatgt ttcggcgcgc catgttgcgc aataaattcc ggtgacgcgc agagaatgcg
    10681 ataattggtc gccagtttgc gggcgattaa attaggggcg atgtcatcgc caatgcgaat
    10741 atcgagatcg acaccttcat tgaccaaatc gaccaaccgg tcttccacat caaaacgtaa
    10801 ttccagttgc ggatacgcct tcgccagcgc tgataacgcc ggggccacca cctgtcgtcc
    10861 aaaaccaaag ctgctgataa tacgcagcat cccctgtggc acctgacgca cgtcagaaag
    10921 ttcgtccatc atctgaccga catcctgcaa aatccgctgc gcccattcat aaatccgctc
    10981 tccttcttcg gtaatggtga cgcggcgggt ggtgcggtgt aacaacacca cgtttagcgt
    11041 ttgctccagc aaggcgatgc gcttgctgac gaacgccgga gaaacgccca gttcttccgc
    11101 cacggcggca aaaccagccc ggcgagccac cagcataaag acgcgcaaat cgttcagcag
    11161 cggtaaatta ttcatgattc gtgttttatg tttcaccagt tacagggatt aattcctttt
    11221 cagtcaatta taggatggtg atgttgtcaa ttttgatggt caggaagtga gaacccaatg
    11281 atgaaaacga tgcgtattgc tgcgatcccg ggagacggga ttggcaaaga agtccttcct
    11341 gaagggattc gcgtgttaca ggctgccgct gagcgctggg gcttcgcctt gagttttgag
    11401 caaatggagt gggcgagctg cgagtattac agccatcacg gtaaaatgat gccggacgac
    11461 tggcatgagc aacttagccg tttcgacgcc atctattttg gtgccgtcgg ctggccggac
    11521 accgttccgg accatatttc gttgtggggt tcgctgctga aatttcgtcg tgaattcgac
    11581 cagtacgtca acctgcgccc ggttcgtctc tttcctggcg ttccctgccc gctggcggga
    11641 aaacagcctg gcgacatcga tttttacgtg gtcagggaaa acaccgaagg cgaatattcc
    11701 tcgctcggcg gcagagtgaa tgaaggtaca gagcatgaag tcgtcattca ggaatcggta
    11761 tttactcgtc gtggtgtcga tcgtattttg cgctatgcct ttgaacttgc gcaaagccgc
    11821 ccacgtaaga cgctaacttc tgccactaaa tcgaacggtt tagccatcag catgccgtac
    11881 tgggatgagc gagtggaagc aatggctgag aattacccgg agatccgctg ggacaagcag
    11941 catattgaca ttctctgcgc acgttttgtg atgcaaccgg aacgcttcga tgtggtagtg
    12001 gcgtccaatt tgtttggcga tatcctttcc gatcttggcc cggcctgcac cggcaccatt
    12061 ggcattgccc catccgccaa cctgaatccg gaacgcactt tcccgtcgct cttcgagcct
    12121 gtccacggtt ccgcgccgga tatctacggg aaaaaaattg ctaaccctat cgccacaatt
    12181 tgggccggtg caatgatgct cgattttctc ggcaatggag atgagcgttt ccagcaagcg
    12241 cataacggta ttctggccgc gattgaagaa gtgattgatc acgggccgaa aacacctgat
    12301 atgaaaggca atgccaccac gccacaggtt gccgacgcga tttgcaaaat tattttgcgt
    12361 taaggtcaaa ccagtttatt tgaaccgcgt cactgacgcg gtttttttat tcgttctttg
    12421 cagtaaataa cctgcgtcat ttcacctttt attgtttccg tttcgtgttt tatggctttc
    12481 cgtattctta attgtttaat ttatgtaaca tgcaaataat caatttgtta atcacgacat
    12541 acaactcatt accctgcaaa taattacaac tggcaaaagc cagagacggg cttttatgcc
    12601 caggagatga ctcatgatga gcaatgttaa gaaaaaagat gtgccgctga taagcatcag
    12661 cctggtggcc attcttttca tcgcagctgc attaagcctt ttcccacaac aatcggccga
    12721 cgcggccaac gcaatataca cttttgttac gcgtacgtta ggttccgccg tacaggtatt
    12781 ggttttgctg gcaatgggac tggtgattta tttagccacc agtaaatacg gcaatattcg
    12841 tcttggcgaa ggaaaaccgg aatacagcac gctctcatgg ctgtttatgt ttatttgtgc
    12901 cggtttaggt tcttctacgc tttattgggg ggttgctgaa tgggcctatt attatcaaac
    12961 gcctggatta aatatcgcac cgcgttcaca acaggcactc gaatttagcg ttccctactc
    13021 cttcttccac tggggcatca gcgcctgggc aacttatacg ctggcctcat taatcatggc
    13081 ttatcacttt catgtgcgga aaaataaagg gctgagcctt tccggcatta ttgctgctat
    13141 taccggcgtt cgcccgcaag gcccgtgggg aaaactggtc gatttgatgt tcctgatcgc
    13201 cactgtcggc gcactgacca tttcccttgt tgtcaccgca gcaaccttta cccgtgggct
    13261 ttccgcgctg accggtttac ccgataactt taccgtacag gcatttgtga tcctgctttc
    13321 cggcggcatt ttttgcctaa gctcgtggat tggtatcaac aacggtttgc aacgtctaag
    13381 caaaatggtt ggctggggcg cgttcctgct gccattactg gtgctgattg tcggtccaac
    13441 cgaatttatt accaacagca tcatcaatgc catcggcctg accacgcaaa acttcctgca
    13501 aatgagctta ttcaccgatc cgcttggcga tggttcattt acccgcaact ggaccgtttt
    13561 ctactggctg tggtggatct catacacgcc tggcgtagca atgtttgtca cccgcgtttc
    13621 ccgcggtcgt aagattaaag aagttatctg gggactgatc ctcggcagca ccgtcggttg
    13681 ctggttcttc ttcggcgtaa tggaaagcta tgccattcac cagtttatca atggcgtaat
    13741 caacgtccca caggtgctgg aaacactggg cggcgagaca gctgtgcagc aagttctgat
    13801 gtcgttgcca gccggtaaat tgttcctcgc cgcatacctg ggcgtgatga ttattttcct
    13861 tgcctcgcat atggatgcgg tggcctacac catggcggca accagtacgc gtaatctcca
    13921 ggaaggtgac gatcctgacc gtgggctgcg tcttttctgg tgcgtggtaa ttaccctgat
    13981 cccgctttca attcttttca ctggcgcgtc gctggaaacg atgaaaacca ccgtcgtact
    14041 cacagccctt ccgttcctcg tcattttact ggtgaaagtc ggcggattta ttcgctggct
    14101 gaaacaggat tacgccgaca ttccggctca tcatgttgaa cattatctcc cgcagacacc
    14161 ggttgaagtc ctggaaaaaa cgccagtgct ccctgcggga accgtattca aaggcgacaa
    14221 ctgagcgcca tcatcctaac gataaaggta tccctatgag caatctgagc cctgactttg
    14281 tactacccga aaatttttgc gctaacccgc aagaggcgtg gaccattcct gcccgttttt
    14341 ataccgatca gaacgccttt gaacacgaaa aagagaacgt cttcgccaaa agctggattt
    14401 gcgtcgctca cagcagcgaa ctggcgaatg ccaatgatta tgtgacgcgt gagatcattg
    14461 gcgaaagcat tgtgctggta cgcggtcgtg ataaggtttt gcgcgcgttc tataacgtgt
    14521 gtccgcaccg tggtcatcag ttgttgagcg gcgaagggaa agcgaaaaat gtgattacct
    14581 gcccgtatca cgcatgggca ttcaaactcg atggcaacct ggcccatgca cgtaactgcg
    14641 aaaacgtcgc caatttcgat agcgacaaag cgcaactggt tccggtgcgt ctggaagaat
    14701 atgccggatt cgtcttcatc aacatggacc ctaacgccac cagcgtagaa gatcagttac
    14761 ccggtctggg cgcgaaagtg ctggaagcct gcccggaagt ccacgatctg aaactggcgg
    14821 cccgctttac cacccgcacg cctgccaact ggaagaacat tgtcgataac tatctcgagt
    14881 gctatcactg tggtccggcg catccaggtt tctccgactc cgtacaggtt gatcgttact
    14941 ggcacaccat gcacggtaac tggacgctgc aatacggttt cgccaaaccg tcagaacagt
    15001 ctttcaaatt cgaagagggc acggatgcgg cattccacgg tttctggctg tggccgtgca
    15061 cgatgcttaa cgtcacaccg atcaaaggga tgatgacggt catttatgaa ttcccggtgg
    15121 attctgaaac taccctgcaa aactacgata tttacttcac caatgaagag ttaaccgacg
    15181 agcaaaaatc gctgattgag tggtatcgcg atgtgttccg tccggaagat ttacgtctgg
    15241 ttgaaagcgt acagaaaggg ctgaaatcgc gtggctatcg tggtcagggg cgcatcatgg
    15301 ccgacagtag cggtagcgga atttccgaac atggtatcgc ccatttccat aatctgctgg
    15361 cgcaggtgtt taaggactaa tgacatcggc ggcggtattt tccgccgctg ggctgatttt
    15421 tgatggagta cagcaatgtc agactatcaa atgtttgaag tacaggtgag tcaggtggaa
    15481 ccgcttaccg aacaggtgaa acgcttcacg ctggtggcaa ccgatggcaa accgctgcct
    15541 gcgttcaccg gaggaagtca catcatagtg cagatgagcg atggtgataa ccagtacagc
    15601 aatgcgtatt cactactgag ttcaccgcat aacacctcta gttatcagat tgccgttcga
    15661 ctggaggaaa actcgcgcgg cggttcccgc tttttgcatc agcaggtaaa agtgggcgat
    15721 cggttaacga tttcaacgcc taataacctg tttgcgctaa ttccctcggc cagaaagcat
    15781 ctgtttatcg cgggcggtat tggtatcacc cctttcctgt cgcacctggc agagttgcaa
    15841 tacagcgacg tcgactggca gctacattac tgcacgcgaa atccagaaag ttgcgcattt
    15901 cgtgatgagc tagtccagca tccacaggct gagaaagttc acttacatca ctcatcaacc
    15961 ggaacacgac tggaattagc gcgattattg gcggatatcg aacctggcac acatgtttat
    16021 acctgtggcc ccgaggcgct aaatgaagcg gtaagaagtg aagctgcgcg tctgaacatc
    16081 gccgccgata cgctgcactt tgagcaattt gctatcgaag acaaaaccgg cgatgcattt
    16141 accctggtgc ttgcccgttc cggaaaagag tttgtggtgc cggaagagat gactattttg
    16201 caggttattg aaaataataa agcagcgaaa gtggaatgtt tatgtcgtga aggggtatgc
    16261 ggaacctgcg aaacggcaat actggaaggt gaagctgacc atcgggatca gtattttagc
    16321 gatgaagagc gtgccagcca gcaaagtatg ttgatctgtt gttcgcgcgc gaagggtaaa
    16381 cgcctggtgt tggatttgta gtttgccgaa gccggactga gcgcgcccag tccggcttcg
    16441 gaagatttta ctgcggatat tcctgcaata aattgtgtaa tgcttccgcc atcagctcac
    16501 cacgccagcc ggaaatcagc tccggcaaat tgttctgcgg tttcagtttc cagtgccagt
    16561 tcagcagttg gttgatttgc cgacgcgatg ccagcaattc ggcgctgatc ttgtgcgttt
    16621 cgctcacgtc agtaatcagc gacttaatcg ctttaaacgc tttacgataa cccggcatgt
    16681 ccatcaggtt aagcatcggc tgcggtaagg cctcttccgg caatgcctgc gctttttcca
    16741 ccagcgccag cagcgtttta ccgtgaaagc ggatttcgct accggataag cccaggctgt
    16801 ccagttcgcc taaacttccc ggcatataac gcgctacgga ccacaaatgc tcttcacgca
    16861 cgacaaagtt cactgccaga tcgcgctccc gcgccttgcg cagtcgccag tcggctaaca
    16921 gttgcagaca ggccagttgg cgtgtgcgta actgccaggc attggtgata tcacgccagg
    16981 catcttccgg agcaacgact tcctgacgac gcatttgcat caggcggcat tcatccagcg
    17041 ccgcaggtag ccagccggag gcctccgttt ctaccataag ctttgcggtg atcggtaaca
    17101 gataccagac atccgccgct gcgtattcac actgacgttc ggtcagcggt ctggccagcc
    17161 agtcggtgcg cgattcactc ttgtccagcg taacgccgga atactcttcc accatggaag
    17221 cgaaacccca tgacatcggg cgtccgcaga aggcagcaag gatttgcgtg tcaatcaagg
    17281 gttgtggtaa ttcgccaaag acattgagga acacttccag atcttcactg cccgcatgga
    17341 gaaattttgt gatggacgga tcgcgcagga tcgctttcag cggtgaccag tcagagatcc
    17401 cgaggggatc aattagcgcc agatgctcac catcaaaaag ttgaattaga cccagttgcg
    17461 ggtaataggt gcgcgtacga acaaattcag tatccagggc tatcgccgga aaggcacgga
    17521 cggcttcaca caaagaagcc agcgcatcgt ccgtggtaat catttggtaa ttcaaattgt
    17581 attctcttta gtgggcgtca aaaaaaacgc cggattaacc ggcgtctgac gactgactta
    17641 acgctcaggc tttattgtcc actttgccgc gcgcttcgtc acgtaattct cgtcgcaaaa
    17701 tttttccgac gttagatttc ggtaactcat cacgaaactc caccagcttc ggtactttgt
    17761 agcccgtgag ctgacggcgg caaaaagtca ccagtgactc ttcggtaagc gatggatctt
    17821 ttttcacaac gaagattttc accgcttcac cactggagcc ggaaggtacg ccaacagccg
    17881 cgacttcctg tacgccagga tgctgcatga cgacatcttc aatctcgttg ggatagacgt
    17941 taaaaccgga aaccagaatc atgtcttttt tacgatcgac aatgcgcagg aatccttctt
    18001 catccatcac cgcgatgtcg ccggtgtgta accagccatt tttgatgatt tcatcggtag
    18061 catccggacg ctgccagtaa cccagcatca cctgcggtcc tttgacacaa agctcacccg
    18121 gttgaccagg tggtacttca ctatcatcat catccaccag tttggcttcc gtcgacggca
    18181 ccggcaaacc gatgctacca ctatgataat caatatcata cgggttaacg ctcaccagcg
    18241 gcgcacactc ggtcaggcca taaccttcca gcagatactg tccagtcagt ttaacccaac
    18301 gttctgccac cacttgctga acaggcatcc ctccgcctgc ggaaagatgc agactggaga
    18361 aatccagctg ctggaactct ttattgttca gcaacgcatt gaacaaggtg ttaacgccag
    18421 tgatagcggt aaacggatat ttcgctaact cttttaccaa ccctggaata tcgcgcgggt
    18481 tagtgataag caggttctgc ccccccagtt cgataaacag caggcagtta atggtcagtg
    18541 caaaaatgtg atacagcggc agcgccgtca ccaccagctc tttgcccgga tgcaacagcg
    18601 gaccataggt cgcgttaacc tgttccaggt tcgccagcat attgcggtga gtcagcatcg
    18661 cgcctttcgc cacaccagtg gtgccgccgg tgtattgcag gaaagctaaa tcttccggca
    18721 ccagttcggg tttgacgtac tgcatccggt agccgttgtg cagggcgcta cgaaatgaaa
    18781 tggcatctgg cagatggtat ttcggcacca gacgcttgat gtatttaaca acgaaattga
    18841 ctaccgtgcc ttttgccgta gatagctgat cgcccatacg ggtcagaatt acgtgctgaa
    18901 cggcggtttt atcaaccact ttttccagag tgtgggcaaa gttagacacg ataacaatcg
    18961 ccgatgcgcc gctatcgtta agctgatgct caagttcacg cggggtatac aacgggttaa
    19021 cgtttacgac aatcatcccg gcacgcaaaa tgccaaacag cgccaccgga tattgcaata
    19081 aattaggcat catcaacgca acgcgatcgc ctttcttcag tcccaaccct tgttgcaaat
    19141 aagcggcaaa cgcgcgactg cgttcttcca gtttgcggaa ggtcatcacc tcccccatat
    19201 tcacaaacgc gggttgatcg gcgtagcgcg cgaccgactg ctcaaacata tctaccagag
    19261 attgataacg gtcagggttg atctccgtcg ggacgtccgc gggataacgg ttaagccaaa
    19321 ccttcttcaa ttcttcacct ctaaaatgcg tgttcgtcgt catcgcaacc ccaaatgata
    19381 tacatgctgt taacataata ttaactcatc ataccagctt gataattacc caacgaaaag
    19441 gttgcgaagc gcgtcactat ttatttttat ctttaccgta agaatgcaga aacagcggac
    19501 cagccgctgt ttctttttct ttaaaaacaa gcaattattc agttacaaca gtttgtactc
    19561 tcgcggggcc aggattatac cagccccatc caccgtagcc atagggccag ccacgaccgc
    19621 cataaaacca tggatcaatc ggctgaggcg gcataatcac ctgctgggtt aaatgccaac
    19681 gtttgtaacc cgttacctgc atcaccataa atttataggg cgtgttgccg attttgccgt
    19741 caaccgcacc ggtgattggc ccaactaccg taaccagttg tccacggaaa tctaccgggt
    19801 ccagaaaacc gttcacatca gcataaatgc gaccgcgaga aggttctccc agcgtcggtc
    19861 tggctccgct gtccagtggt acggtggcaa tttccaggcg ggttttccct tgctggtttt
    19921 gtaccgcaac cactttgcca ccaaagcgtg cctcctgacc aacgtacagc tgcggcgcac
    19981 tcatcacccg aactaaatct tgttgcggcg tgggactgct gcctttaatg gcgtccggca
    20041 cagtaacaca accgctgagc attagcgcaa acgtacctgc cagtatgcct ttgataacat
    20101 tcttttgaac cgccatgatg cgactccttt tttctcaggg catactctta agattcattc
    20161 tttgcccgga agtttcttcc atgcgacgtt gttacgtaaa taaaccggtt cggcatgttc
    20221 caccgccacc gttttaccct cggcaaacat ctgacacgca atcggaagca tatcttctgc
    20281 agcgggcagt aacacttcgc catcgcacag ggtcaaaccg ctctctttac cgagatccgg
    20341 ccaggcttgc cagcctgtac ctaccgtcac ccattccccg gaaagctgtt gcatccgttc
    20401 atgaacgagt tcgggtttga gtacggcttc ggtttcttca ccgtgccaga tacccttttc
    20461 atcacgctga tattcggccc agtaaacttc gcccatccgc gcatcaatag cagccagcac
    20521 gcgggttgcg ccgtttttgc gccacgcccc ttgcgccatt gtcatgagcg tggaaacgcc
    20581 aatcatcggc agatccgcgc caagcgccag cccctgcgca ataccaatac caatgcgcac
    20641 gccggtaaag ctaccggggc cgcgcccgta agccagagcg ttaatatcag tcagggaagt
    20701 tccgctggtg gtcaggatat cctgcaccat cggtaagatt cgttgagtat gttcacgagg
    20761 gcaaagctca aaatgagcgt tgacagtacc gtcgttccac agggcgacag agcaggcctc
    20821 tgtcgcggta tcgatagcca gaattcgcat gggtcttcgt gcttagatca ataaaaaggc
    20881 gcgcatcata ccatactccg taacaaatta cctggaggat ggtatcgcaa ggaaacgaac
    20941 cgcacgggca atgtcacggg tgcgcggcgc tggcggcaga ctggcgagaa acgtcgcgcc
    21001 gtaagggcgc atcaccagcc gattatcgca aatcaccagc acgccgcgat cgtcggcgtc
    21061 gcgaatcagt cgccctactc cctgcttgag agtaatcacc gcatccggca gttgtacttc
    21121 atcgaacggg tcaccaccac gcaaacggca atcttccatg cgcgctttta atagtggatc
    21181 atccggtgag gtaaacggca acttgtcgat aatcaccaat gacaatgtat cgccacgcac
    21241 atccacccct tcccagaagc tgctggttgc cacaagaagc gcgttaccgg cgctgacaaa
    21301 ttgctgcaac agttgccctt tgctggtttc cccctgcaac aaaacgggaa gcgtcatggt
    21361 agcgcggaac tgctcagcca gatcgcgcat catggcgtgc gaggtacaaa gcataaaaca
    21421 acgaccgttg ttagcttcga tgatcggtcg caacattgcc gccagttgcc gtgcggagcc
    21481 cggttgattg gtttgcggca ggttgcgcgg cacacagagt aacgcctggc ggctgtaatc
    21541 aaacgggctg ggtaacagca acgactctgc ctgttcaatg ccaagccgcg aggtaaaatg
    21601 atgcagatcg tcgttcaccg acagcgttgc tgaggtaaag atccagctac cgggtttttg
    21661 cgccattaac tctttgaatt tatccgccac gctgagcggc gtgagcgcca gagtaaaatg
    21721 gcgcgaagtg cattcgtacc agtagctgta gcccggctga ttgatctctt ttagccgttt
    21781 cagccgcgta cgatacaacg tggcgcgctc aaatgccgca tccagcaagg cggaacgccc
    21841 cagcgacagt ttcgccacgt cataacaaag ttccagggtg tcatcgagca gtaaaaacgc
    21901 ccgttgaatt tgcggattag ctaacagttc gcgcaggttg ccacgataac caggctcacc
    21961 gagttgcaga cgaaaatcct gcgcgctctg ggcaaggcgg tcggcgcact tttgtaactg
    22021 ctgagtatct tttaattcgg tgcgataggc gatggtgatg tcttttgcca ggtcgagtag
    22081 ttgtcgactg gagagtgact gaccaaaata ctggctggca atgtcaggca gctggtgggc
    22141 ttcgtcgaag atcatgacgt cagcttccgg gatcagttcg ccaaatccac tctctttcac
    22201 caccatatcc gccagaaaga gatgatggtt taccaccacc acatcggcgt ccatcgcttt
    22261 tttgcgtgct ttgaccacaa agcaatcttt atacatcggg cagtcgctgc caaggcagtt
    22321 atcgttggtg ctggtgacca acggccacgc ctgtgaatct tccgccacgc tgacgcaggt
    22381 gctgatatca ccatcgactg tttgattaga ccaggagcgc agcaggatca catcgcttaa
    22441 gatttgtacc ggcagatcgc cccccgccag cgcctgctgt tcgagacgtt cgaggcagag
    22501 gtagtttgag cgccctttca gcaacgccac gttgcccgta tatttcaacg cctttgaaac
    22561 cgttggcaaa tcgcggctgt agagctgatc ctgcaacgct tttgagccgg tcgagataat
    22621 gactttcttt ttcgcccgca gcgcaggggc caggtaagcg taggttttgc ccgtaccggt
    22681 tccggcttcc accaccagcg gctggccttt ttctatcgcc tgggtgacgg ctaccgccat
    22741 ctgtcgctgt ggttctcgcg gcttaaagcc tggtatcgct ttcgccagct gaccgtctgg
    22801 tgcaaaatcg tccgtcacac taccccctgt tgatttgaac agggattatg tcaggatgag
    22861 ggcgctttcg ccagttgaag tggtgacggc gacctcacat tgtggcagtc tttgcagcac
    22921 gaaaatggaa gtataatgag gacaaaatga tgactatcgt tcgtatcgat gctgaagccc
    22981 gctggtctga tgtagtaatc cacaacaaca cgctctacta cactggtgta ccggaaaacc
    23041 tcgacgccga tgcctttgag caaaccgcca acacgctggc gcagattgac gccgtgctgg
    23101 aaaaacaggg cagcaataaa tcgagcattc tggatgccac cattttcctg gccgataaaa
    23161 acgacttcgc ggcgatgaat aaagcgtggg atgcctgggt tgtcgcgggt catgcgccgg
    23221 tgcgctgcac ggtacaagcg ggtttgatga acccgaagta taaagttgaa attaagattg
    23281 tggctgcggt gtaagcttta tcgaagcaaa ataagtcaga cgataattta tcgacaatac
    23341 tggtcggttt tgcataaatc gaccagagat ta
//
LOCUS       DAZKJR010000080        22724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKJR010000080 DAZKJR010000000
VERSION     DAZKJR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.080"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..552)
                     /locus_tag="ACHOWS_003101"
     CDS             complement(<1..552)
                     /locus_tag="ACHOWS_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFQ0048644.1"
                     /translation="MAFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPSDGEGTVISSTSEPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGI"
     gene            813..1148
                     /locus_tag="ACHOWS_003102"
     CDS             813..1148
                     /locus_tag="ACHOWS_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFQ0048645.1"
                     /translation="MKWIVIDTVIQPSCGISFSAIWSKIKLIIWYQSDVFLPPESIFT
                     LTHTGIVLNNKVLPVTIYNVVPFNKRFWDLIKNSQECPANSDNVLNECFNNHCILQIC
                     PYGLKQQSP"
     gene            complement(1789..1865)
                     /locus_tag="ACHOWS_003103"
     tRNA            complement(1789..1865)
                     /locus_tag="ACHOWS_003103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1829..1831),aa:Arg,seq:tct)
     gene            complement(1879..1955)
                     /locus_tag="ACHOWS_003104"
     tRNA            complement(1879..1955)
                     /locus_tag="ACHOWS_003104"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1919..1921),aa:Arg,seq:tcg)
     gene            complement(2104..2856)
                     /gene="ydfT"
                     /locus_tag="ACHOWS_003105"
     CDS             complement(2104..2856)
                     /gene="ydfT"
                     /locus_tag="ACHOWS_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFQ0048646.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARHRVQKLIPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEEQLRKVTR"
     gene            complement(2870..3859)
                     /locus_tag="ACHOWS_003106"
     CDS             complement(2870..3859)
                     /locus_tag="ACHOWS_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFQ0048647.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPNLVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(3867..4709)
                     /locus_tag="ACHOWS_003107"
     CDS             complement(3867..4709)
                     /locus_tag="ACHOWS_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFQ0048648.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQITELVQELIDTQNLGVGSFNETTQNLGSKPVSKIEGRNGGTYVCKELVYAYAM
                     WISPSFHLKVIRTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQ
                     KLQEAVGLPNLAPRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLG
                     IVEQRERYSRTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLL
                     DTVH"
     gene            complement(4729..5118)
                     /locus_tag="ACHOWS_003108"
     CDS             complement(4729..5118)
                     /locus_tag="ACHOWS_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFQ0048649.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRELDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIQKGKVEITISKYEKPAGAAA"
     gene            complement(5115..5441)
                     /locus_tag="ACHOWS_003109"
     CDS             complement(5115..5441)
                     /locus_tag="ACHOWS_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFQ0048650.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            complement(5441..5935)
                     /gene="yfdN"
                     /locus_tag="ACHOWS_003110"
     CDS             complement(5441..5935)
                     /gene="yfdN"
                     /locus_tag="ACHOWS_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFQ0048651.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQSVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLFRRAATVWMEAFRESHSQPERNNFLARRERCLRKSSKRAVSGDEWYLSGN
                     YVGA"
     gene            complement(5932..6750)
                     /locus_tag="ACHOWS_003111"
     CDS             complement(5932..6750)
                     /locus_tag="ACHOWS_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0048652.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSPGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(6747..6983)
                     /locus_tag="ACHOWS_003112"
     CDS             complement(6747..6983)
                     /locus_tag="ACHOWS_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048653.1"
                     /translation="MLNPLSLNIRRLLQRKKTSIPTVGQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLITFCSGKNMKHLGGAA"
     gene            complement(6976..7686)
                     /locus_tag="ACHOWS_003113"
     CDS             complement(6976..7686)
                     /locus_tag="ACHOWS_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFQ0048654.1"
                     /translation="MRFSEIAAVYFHALNKMLVSGTHTQWKLIDLQSDLFTVVFDSIV
                     HELKHNKIVMFAQCIDSVIPGCVDNIYLSGHTLHIVAKSTIGSETPELSTAIYDAPAS
                     FLSSALTHARIMVGWAGEPKGSPVSVDAGSLNPVQSATSEIETSGGGSFPQSTEAAIM
                     ATVPAITRLNDEDLHKLSYITTALRALRKVTLSDPQAHQVLVETILNLQAERIRLADK
                     ANFHIHRLLNISGGHRHA"
     gene            7651..7881
                     /locus_tag="ACHOWS_003114"
     CDS             7651..7881
                     /locus_tag="ACHOWS_003114"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0048655.1"
                     /translation="MKVYGSNLGEPHTCAFGDGLFELRIKGSDGIARVFYCTLTGKRI
                     IMLHSFIKKTQKTPPAERKKAETRMKEVKHDW"
     gene            7871..8179
                     /locus_tag="ACHOWS_003115"
     CDS             7871..8179
                     /locus_tag="ACHOWS_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0048656.1"
                     /translation="MTGKRTPPTMTHDEMAAKWMDDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGVSGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(8279..8374)
                     /locus_tag="ACHOWS_003116"
                     /pseudo
     CDS             complement(8279..8374)
                     /locus_tag="ACHOWS_003116"
                     /inference="COORDINATES: protein motif:HMM:NF025339.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(8520..9071)
                     /gene="ymfL"
                     /locus_tag="ACHOWS_003117"
     CDS             complement(8520..9071)
                     /gene="ymfL"
                     /locus_tag="ACHOWS_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFQ0048657.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQFLPLGWAMVLQRAAGTHHIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEAIEQIGSYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            complement(9115..9315)
                     /gene="ymfT"
                     /locus_tag="ACHOWS_003118"
     CDS             complement(9115..9315)
                     /gene="ymfT"
                     /locus_tag="ACHOWS_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0048658.1"
                     /translation="MKAYWDSLTKEQQGELAEKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            9406..10080
                     /gene="ymfK"
                     /locus_tag="ACHOWS_003119"
     CDS             9406..10080
                     /gene="ymfK"
                     /locus_tag="ACHOWS_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFQ0048659.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYQIKA"
     gene            10440..10750
                     /locus_tag="ACHOWS_003120"
                     /pseudo
     CDS             10440..10750
                     /locus_tag="ACHOWS_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10765..11127
                     /gene="yfdP"
                     /locus_tag="ACHOWS_003121"
     CDS             10765..11127
                     /gene="yfdP"
                     /locus_tag="ACHOWS_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFQ0048660.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            11193..12017
                     /gene="yfdQ"
                     /locus_tag="ACHOWS_003122"
     CDS             11193..12017
                     /gene="yfdQ"
                     /locus_tag="ACHOWS_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFQ0048661.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEDMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            12145..12681
                     /gene="yfdR"
                     /locus_tag="ACHOWS_003123"
     CDS             12145..12681
                     /gene="yfdR"
                     /locus_tag="ACHOWS_003123"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFQ0048662.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIIINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPSHA
                     YGMFMERFNELSELRKCA"
     gene            12672..13013
                     /locus_tag="ACHOWS_003124"
     CDS             12672..13013
                     /locus_tag="ACHOWS_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048663.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFTEAEAKISA
                     LAAENELARKAVQAFCDVVGDNTEVIAEEVGRDGVLVILEAMKATGNMPATDAFLAEV
                     RAQGGIWLVTR"
     gene            12995..13126
                     /locus_tag="ACHOWS_003125"
     CDS             12995..13126
                     /locus_tag="ACHOWS_003125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048664.1"
                     /translation="MARNAMIDFVDGEVGPNKNVPGLIRGTEICVSIAEQLRKGGNQ"
     gene            13123..13467
                     /locus_tag="ACHOWS_003126"
     CDS             13123..13467
                     /locus_tag="ACHOWS_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFQ0048665.1"
                     /translation="MSGINYQALREAAQNYQSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTSAGIRIEGE"
     gene            13469..13821
                     /locus_tag="ACHOWS_003127"
                     /pseudo
     CDS             13469..13821
                     /locus_tag="ACHOWS_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13818..13964
                     /locus_tag="ACHOWS_003128"
                     /pseudo
     CDS             13818..13964
                     /locus_tag="ACHOWS_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14604..14846
                     /locus_tag="ACHOWS_003129"
     CDS             14604..14846
                     /locus_tag="ACHOWS_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFQ0048666.1"
                     /translation="MFSLIQRGQIYTDRAGYPVVITRSTEHLVFFRRMDGHSGRVRIS
                     EFNNHFEHIDHQEFRQIQTELEQEAHLKKLRAMKRK"
     gene            15002..15217
                     /locus_tag="ACHOWS_003130"
     CDS             15002..15217
                     /locus_tag="ACHOWS_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0048667.1"
                     /translation="MALNIDCVPITTWCRDMGETLDAVNKRLQRGVWKEGVQVLNVEG
                     SKERWIDLTEVSKWARKNKYPYHSQEE"
     gene            15172..16344
                     /locus_tag="ACHOWS_003131"
     CDS             15172..16344
                     /locus_tag="ACHOWS_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0048668.1"
                     /translation="MGEKKQISLSLPRGITIRQYKNCSTLVVTFTYKGVLCREPLPRL
                     EANVKGIKYAERLLGEIKNQISDNSFDFSKYFPNSRKLALFGMVKKNKSVRDYLEEYL
                     EICKNRKLSPSTINGYEKCVSALSELHKLHVVDLTPAILKKWISKRTTKLKTIRNNLS
                     FLRSAVDEAVTDGLITINPVKLVSASRYHAVNHTANADDYEVDPFTPAEATAIYNACI
                     YQEWRNLFCFAFNTGLRSSELCALRWSDIDFIGHTVHVQVASVVGVIKGTKTKAGTRK
                     VELNNEAIMALQAQKSFTMMRSDFVFSDPKTRKPWANSAAIRKKAWIPTLKKAGVRYR
                     NPYQTRHTFATRHISQGANLFWLAGQMGHKGPEMLFRHYGSYLSEYDGKTAISEVI"
     gene            complement(16665..21239)
                     /gene="ypjA"
                     /locus_tag="ACHOWS_003132"
     CDS             complement(16665..21239)
                     /gene="ypjA"
                     /locus_tag="ACHOWS_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFQ0048669.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQKVDERGTTNNTHIINHGQQNVYGGISNGSLIESGGYQDVGGHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLDSKSTAKNTQIYSGGTQIVDYTSTSDVIEIYSGGVLDVRGGM
                     ATNVTQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATDANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSIAGYLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQVLARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     SELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDP
                     KPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHTHIFFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     SLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(21304..21423)
                     /locus_tag="ACHOWS_003133"
     CDS             complement(21304..21423)
                     /locus_tag="ACHOWS_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0048670.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(21578..21844)
                     /locus_tag="ACHOWS_003134"
     CDS             complement(21578..21844)
                     /locus_tag="ACHOWS_003134"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0048671.1"
                     /translation="MLRVTTSAHNRGSRPGQNKRLRTLSVGDALLVCKLNRLGRSMWH
                     LVVLLEELCERGINFRALAQSIFAQQWGDGSCKSKTICDLRVIV"
     gene            complement(22175..22724)
                     /locus_tag="ACHOWS_003135"
                     /pseudo
     CDS             complement(22175..22724)
                     /locus_tag="ACHOWS_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5971 a         5687 c         5164 g         5902 t
ORIGIN      
        1 aatacccgca ttcgccggta taaacggcag cagttttttg gcgatatgaa gcccctgccc
       61 cacggttccg tactgcccct ttgacaggtc cgctttcgga tggttaagac ggctcatgtc
      121 ctgcacatca tgcagacaat ggtccgccgg aataatgtcg ttatacttac aggcggcacc
      181 tcccggtgtc accgtactgc ggcgcgccag ctgcttaata cgcgggtccg gacggtcata
      241 tgtctccggc agcggaaggc cttcaccata tgccatgctg tttgactgcc ccgccagaac
      301 aacaacaaag taatactccg gttcgctggt ggagctgata actgtgcctt ctccatccga
      361 cggcttcacc accacaggtg tgctcacatc accttctgcg acaatcgcct gaataagtgc
      421 tgcgccatca tccgtatacg aagaaaacgg cccgccgtat ggctgccatc cttcacgaat
      481 tttttgagca agcgcatccg caaggtctga cggcgacgcc gccctgacca cgtcatagtg
      541 tttaaatgcc atggttcttt ccaccatatt aaaaacaatt ctttaaaata cctgacatgc
      601 aatacagaaa aaacacaaaa ccatacctta aataaaaact tcatcataaa gcaggtatgc
      661 atggataaac tacaagacga gatataaaca accctgtatt taaataaaca ataaacaaca
      721 tcagaaaaat aattctgctc tatgatttac attcaaaatt atcatttata cttttcagaa
      781 catcaccagc acagcataaa taaggaggct aaatgaagtg gattgtgatt gatacagtta
      841 tccagccatc atgcggaata tctttttcag ccatatggag taaaataaaa ttaataatct
      901 ggtatcaatc ggatgttttc ttacctcctg aaagtatatt tacgctgact cacacaggta
      961 tcgtacttaa taacaaagtg ctacctgtaa ccatttacaa cgttgtacca ttcaataaaa
     1021 gattctggga tttaatcaaa aacagccagg aatgccctgc aaattcagat aacgtattga
     1081 atgaatgctt taataaccat tgcattctgc aaatatgccc ttacggacta aaacaacaga
     1141 gtccataatt agtttactca tatcttacaa aattcatata aaagcccctc cggagagggg
     1201 ctggagtaaa cgctatgtac cgtccattgc atgatgccgg gtgcctcccg gtgagttcag
     1261 cccggtgcca ccaaacccgc gcgttcactt tcctgggtat caaagataac acctaatgca
     1321 ccagtcgccc ctccgcacag ggggattcat catgcaggat ttttttaaca aatgctcagc
     1381 cagacaggca accgtcaact gactgaattg tgagtgattt aacatttcac tgcccggtgc
     1441 cttcccctca ttaaaaagcc cctccggaga ggggctgacg ctgcgtatct gtatcatcat
     1501 acgcatggtg ccgggtgcct cccggtgagt tcagtatcag cacctgaacc cgcgctggat
     1561 aaggataagg gatgtaacca aacacctgtc gctgatatgc ccctccgctc agggggattc
     1621 accatgcctg tttcttttaa caaactcccc gcaaaacaga caactgtcaa ccgtctgaat
     1681 tgtgagacat ttaaaatttt cggggcatga ctgatacccg gctaatcacc tgatgcttcc
     1741 ttttttagca acaaacaggt ccaccaaagt ggacagaaaa aataagtatg gcgcgttgta
     1801 ctggattcga accagtgacc gattgcttag aaggcaattg ctctgtccga ctgagctaac
     1861 aacgcagtgt accgataatg gaccgccatc gaggactcga accccgcgca accagcttcg
     1921 aaggctggcg ctctatcctg atgagctaat ggcggtattt aacaggagat gcaacaaaaa
     1981 cccgctcagc ggcgggctct taaatcttat caacggtaga catacaaagc ccatcgttgg
     2041 gaaaacttta tccatatttt ttgaaaaatg caagcatcat gtcgacatct tcggcgaaat
     2101 tatctatctt gtcacttttc tcaattgctc ttcagcatac gcttcttcct gccagcactt
     2161 tgtaaccagt ttatcaatga catctgcata tcctttgtac cactgataat ccgtcaggtc
     2221 tggtatcagc ttctggacac gatgcctcgc cagtgtggtt ggtaaacggc taaaccggtt
     2281 tccattgcaa cgcccacaaa tcttataaac aggcgcgcca tgaagccggg ttcttttttc
     2341 atccaggaca atacctttac ccttacaccc tctgcacgct gtgctgactt ctcccttacc
     2401 atggcaatgc tgacatagtt ccttcaccca ctcttccttg ataacagatt ccccgcttct
     2461 ggagtgtttc accacttcgc gcaatacatt atgaaatcca gtaccagcac aatgctcaca
     2521 gcgagcctta cttgccgcag accgggaata atcagcaaag gcaaaattca caaggtaagg
     2581 gatgatctgt aaccgggttt cttcactcaa tttattcaat gtcgggttat ccagtgccat
     2641 cgcgtaattg agcagacctt caatcgcaaa ctgaggatcc tgaacaccaa cttttgccag
     2701 gaataaggca aacccaagcg gtgctttcga ctgcaccatc ccctgcgcag ccatcacatc
     2761 tgtaatcgtt aaaccacccg agcctgtcgc cggtgcgtca tcgctcaatt ttggagattt
     2821 tggggagtaa tattttggta aggcttcaag gttcatgctc gttctccact tacgccagta
     2881 cgcctattgc cagcgcacga tcgataaaac gaaatatcag ctccagctgg gagccatact
     2941 tctcttcgaa tgccacggta tccgcatgca gctcgtcgtg atgctttctg cacaaaggca
     3001 acacaaagag gtcatgcgct tttgttccca ttccaccctg accgtgacct atcaggtggt
     3061 ggggatcatc agcgggcttt ccacaacatg cacacggctg tgtcttaacc cagcgcgtgt
     3121 acttttcatt aacccagcgg cgacgttttg ggcgtaacat aaaagactcc ggcgactccg
     3181 gatccacttt cagcgccagc accttttttg ccttatcctg gatgatgctg gtggcaggaa
     3241 ccgaaggcac aaggtcactt tcccgggtga cagacggcac aacaagattt ggtaatctca
     3301 gtgctttacg ggctgcactt tccggtaagg catccgccag gtcattacga atcagccacc
     3361 agcacagttc cggcattgtc acaacatgac tatcatcaaa accgagatcc cgacgcacaa
     3421 cagacaacac ccagcgggca cagttatccg tcgccattga ttccagccgt tccgtgaact
     3481 gatcgcgcag ctggttatcg cagtgccagc acagacggat tgcgcccgga gcgtgtcgca
     3541 ttgtggtcat gttctcgctg tgccagtcgg aatgaggcca ctggcagcct ttttcacgaa
     3601 gtaaccagct ttcaagacat tccacgccac cagcacgacg gatcactgcc tcattgcgga
     3661 acacagcccg aacggcagga tcatccgcca gcggttgtga tgccgccgga acggcaccac
     3721 tggcgaaaga tgaataacgt tccggctcag gctccagcag gacacgcccc tgcataaaca
     3781 ggggcatcag ctctgaaccg ggcctgaaca atacgatccc catacgcggg gcaatttcag
     3841 gggtcagtag tgctctcacg gtcacctcaa tgaacggtat cgagcagctt taacagctca
     3901 gggaatcggg attcgaagaa atgcggctgc gtctcgcgcg gatttgcggg actggtgatg
     3961 tttttgccga acatgcagcc tttcgccgtc agcgaccaga attttttgat gttgttaatc
     4021 gcagtgcggc tgtatcgttc acgttgttca acgatcccca gcttcgccat ctggtgatat
     4081 gcctgattag ccgtcaggcg tataccatac tgtttcagca gtgcactcag tgacagtgtc
     4141 gggcgacttg agccatcgtg tgcatcagca ggagcatcaa tggcatagcg cggtgccaga
     4201 ttcggtaagc caacagcctc ctgaagtttc tgacaggcac caagcactga tgagttagac
     4261 agatttaact cccggcgcat aaagtccagc agaatcacgc cagcctgcat cttgtcagca
     4321 gcctgcccgg ataatttttc cggtgcgctg gttaccatgt cgaaagtgcg gatcaccttc
     4381 agatggaatg acgggctgat ccacattgca taggcataca ccagttcctt acagacatat
     4441 gttccgccat tccgcccttc tattttactg acaggtttac tacccaaatt ttgggtagtt
     4501 tcattgaatg aaccgacacc cagattttgg gtatcgatca attcctgaac cagctcagta
     4561 atctgttggc tggaaagaaa ctttcccggc tccttggttc tggcatttgc accagatgct
     4621 actgctgcgc gatgcagatc gttcaggctg taacgtccat aagcatcacg acgaacttca
     4681 ataccatcaa tgaccatcag attattcata cttcgtttct cctcttaatc aggcggctgc
     4741 acccgccggt ttctcatact tactgatagt gatctcgacc ttccctttct ggataaccgg
     4801 tccccactcc accagcattc ttttcacctg gctgtcgtct tcccacaccc ccgcgtgggt
     4861 cagggcgtca aacagcgcct tgttatagtt gtccagctcg cggatccggt tatccggagg
     4921 aaacaacacg atctccactg aagcaggtgc cgacgttggt tttggcagac gacgtaactg
     4981 ctcaactatt gctgcgcacg ccgcgctctg gaatttgcgc cccgccgcgc ttatcaggct
     5041 tttaccagca aacgcccctt tattagggtg tcgccagtac gtattcacgc tgggcgggaa
     5101 aggtaggatc agtttcatac tttcaggcct ctctcatgta accagtgggt tgcacgcagc
     5161 ctggcgtttt cctcaccggc aagcagtgag cggataatcc cgaccgcctc gctgtcgtcg
     5221 tccttcaccg cggtatgaag cgttatcccc cgggccacgc cacgctttat cgtgatgacg
     5281 ccttttttct ccagtgcgcg aagatgctcc accgctgcat tcactgaacg gtatcccagc
     5341 atggttgcca cctcctgatt ggttggcggg aagccacgtt ctttctggta agaaatcagc
     5401 atatccagca cctgctgctg acattgagtt aacgtcgtca ttaagccccc acgtaattcc
     5461 ctgacagata ccattcatca cccgatacag cgcgcttgct gcttttccgt aagcaccgct
     5521 cacgacgcgc cagaaaattg tttcgttctg gctgggagtg gctttcacgg aatgcctcca
     5581 tccacaccgt tgcagctcga cggaataagc ccctagactc cagttcttca gcctggcggg
     5641 tcaggcacaa aatcacccgg gggtcgttag tgccgacata gaaattgcgc acaggtctgg
     5701 tttcacgaac agattgtggt tccggctcct gcgctctctc agtcaggcgc gggaaatgtc
     5761 tgcgtgtatc tccttcacaa cggtgagcca cacgcccact ctgacgtaac ttgcttgctg
     5821 actgcagaac gcgctgccgt gagtaaccag caaaagcatc cgcaatgtct ccggaagtac
     5881 accccggatg ggcttcaatg aatttctgaa cttcattcaa aagactcata atcaccccct
     5941 gaatcctgcc gggatctggc tgtagtccac gttgtcgtaa ctggctttga agtacgggtc
     6001 ctcgcgtctg gctgcagata ctgcaggaac ttcccaggat tcttcgaaat gacgatccgg
     6061 accaaagaac gtgacagcct gtttcacaaa ttgtgtgccg ctgttaccca tcgcagatac
     6121 ccagcccgcg tagcgtttca caccttccag catggtttcg gggtttaccc cctcattcaa
     6181 acgggctttc caggctttga aggctgcaga ttttgaattg ccaccagcac gtttgggata
     6241 ggccagccat gcctgctcaa actccggaga gtattccggt cggtttgaac gaactcgcac
     6301 agactcatca gcagatgcac caacagctat tggttcattg actggttctt tgactggttc
     6361 aaaagagtga ctggttctgg gtgaatctcc tgcactaccc cctggtgcaa ctcctgcact
     6421 acctggtgaa tttgctgcac cagatagtga attatttgca ctaccccctg gtgaatctcc
     6481 tgcaccatca agatgaagga gatagatatt acttgagtta cctttttcac ctttccgggt
     6541 gacttttttt accagcccgg actcacaaag ggccgcaata tgattcatca cagaacgttt
     6601 gctaatctcg cactggtcag caatatgctg gtagctgggc cagcactccc cctgatcgct
     6661 ggcattatca gccagcttga tcagaaccag ttttcgcaat ggattaccca ctcgaatttt
     6721 catcgcttta accatcagct ccatactcat gctgcacctc cgagatgctt catgtttttt
     6781 ccggagcaaa aggttataag cggcatactg acgcggtaat tacggcccag cggttcacaa
     6841 atcactttct gacattcacg gtcaaccagg ctaacacgta gaacatgccc tgcgggcgtg
     6901 gtgtaccact gcccaactgt aggaattgac gtttttttac gctgaagtaa acggcgaata
     6961 ttgaggctca acggattaag catgacgatg ccctccgctg atattcagga gacggtgaat
     7021 atgaaaatta gccttatccg ccagacgaat acgttcagcc tgcaagttaa gaatggtttc
     7081 taccagaacc tgatgcgcct gcggatccga aagagttacc ttgcgcagag cacgtagtgc
     7141 agttgttata taactgagtt tatgtaagtc ttcatcattc agacgagtga tggctgggac
     7201 agtagccatg atggcagcct ccgttgactg tggaaaactt ccaccaccgg aggtctcaat
     7261 ctcgctggtg gcggactgga cagggttgag actaccggcg tcaacggaga ccggcgagcc
     7321 tttcggctcc cctgcccagc ccaccataat tctggcgtgc gtgagcgcgg acgataaaaa
     7381 agacgctggc gcgtcgtata tcgccgttga caattccggg gtctcactcc cgatagtgga
     7441 ttttgccact atatgtaaag tgtgccccga caaataaata ttgtcaacgc atcccggaat
     7501 tacagaatca atacattgcg caaacataac aattttgtta tgtttcaatt catgaactat
     7561 actatcgaat actacagtga agaggtcaga ctggaggtcg atcagcttcc attgagtatg
     7621 cgtgcccgat accagcatct tgttgagcgc atgaaagtat acggcagcaa tctcggagaa
     7681 cctcatacct gcgccttcgg tgacggactt ttcgagcttc ggattaaagg cagtgatggc
     7741 atcgcacgcg tcttttactg caccctgaca ggaaaacgca tcatcatgct gcatagcttc
     7801 atcaagaaga cccagaaaac cccaccagct gaacgcaaaa aagctgaaac cagaatgaag
     7861 gaggttaagc atgactggta aacgtacccc gccaaccatg acacacgatg aaatggcagc
     7921 taaatggatg gatgacccgg cctttaaagc agaatacgac gccatcgccg acgagttcgc
     7981 actgcttgat gaaatgctgg ccgcacgcaa agaagctggt ctcacccagg ctgaagttgc
     8041 tgaacgcatg ggaactaaag caactgccat caccagaatg gaaagcaatc ttgcctctgg
     8101 tgtcagtgga ccatcatttg ccacactgaa aaaatttgca cgcgcaacag ggaaaaaact
     8161 ccagatccgc ttcgtgtaaa tttccattat tgcgccgtta ttatggcggc gcttcgcact
     8221 caacaaaatc acgcctgaat ttccacaaag gactaaagca ctcatgcggg tagtctttgc
     8281 gaagatagat aacgcgctgt gtttctggct cccaacgaat aacatggaca taaagccctc
     8341 ttccgtcacg aaaccagcgg ttaagttcct gcacaacttg ccccccacag tcaggtaaag
     8401 ttctctgtgg ttacttacag ccaggtgatt tggtaatctg cattcatgcc gtaacaacag
     8461 gtgttcagcg acgctgacca ccagctgttg cgacaaacgg ttatttgccg ttaaactgtt
     8521 catgcgttag tttctccaca gacacaaaac gccacgacgc ccggagctgc acactcgcgg
     8581 gcgtcactct tttctggagc gcaaaagatt ttgtagacca gtgctgcatg ctcctggagc
     8641 ttcgaaattg acagatacaa ctcatcatta attgctgtct gctcgtgtgg ctccactacc
     8701 ccgtcttcga ttgccgaacg aatctgcttt gagtaactac cgatctgttc gatggcttcc
     8761 agcaatcgct ggttgatatc ggcgttctct acttcctcaa tttcaggaag cgatacaaac
     8821 accccaccag cagactgtgc gacagcatcc gcaatgtggt gagtaccagc cgcacgctgt
     8881 aaaaccattg cccatcccaa cggtaaaaac tgatcgccat cggcacgaag gcgattaaat
     8941 aatgcgttct ctgttacatc cagccagtca gccgcttcag cgtaaccccc cggcaacgcc
     9001 gcgatagttt ttctgacagc tttcacgtac cactctggct gtttttctac tttccagtga
     9061 tgcttaccca cggttagcct catcgttctg tggttaaaaa ttgaaggtgt tctgttaatc
     9121 tttcggatag atatccggtc ttaagtcaga tttcgtaatt gcacctgacg tgcattgctc
     9181 aagtttttta gccagcacaa aactggcttt tttataacca ttgaaaacca gccgtaagta
     9241 gcctggtgtt gagccaactt tttcggccaa ctcgccctgc tgttctttgg ttaaagagtc
     9301 ccaatacgct ttcatacaat atgtacctcc cgtatacata ttacatgatt gagatgaacc
     9361 ttcaagatac ttgtacctta tcggtacaaa ggttttaatt tctttatgaa aacagtccat
     9421 gacatccggc ggtctaacgc cagaaaactg agagatggtg ttggcgggaa ttcttccttt
     9481 gccaccatga ttgatcgcga gccaacccag accagcaggt ttatgggaga tggtgctact
     9541 aaaaatatcg gtgacagcat ggcacggcac atcgaaaaat gtttcgacct gcctgtcgga
     9601 tggcttgatc aagaacacca gaccacgaac atcacaaaaa aacctgatgt ttcaatcact
     9661 aataaacaaa taacgttagt ccctgtcata tcatgggtac aggccggagc atggaaagaa
     9721 gttggctatt ctgaggttga tttgagcaca gcagaaactt atccctgccc tgtaccctgt
     9781 ggcgaaatga cttatatctt gcgggtgatt ggtgattcaa tgattgatga gtaccgcccg
     9841 ggagacatga tttttgttga tcccgaagtc cctgcctgcc acggtgacga cgttattgca
     9901 ttgatgcacg atacaggcga aaccaccttc aagcgattga tagaagatgg aacacagcgt
     9961 tatctcaaag cattaaaccc aaactggcct gagccttaca ttaagattaa cggtaattgc
    10021 tctataattg gtacagtgat tttctcggga aaaccaagaa gataccaaat aaaagcctaa
    10081 tcaatattta taacctgctt cggcaggttt ttttatactt gacaatgtac ccgatagata
    10141 cataatgtat ctaaaagaaa catgccgcag gcgagattca aacaaagttt ggttgtaaca
    10201 cggcgtatgg cacatgcgtc gttagcggtc tggggacgtt aaaggggaca atccactcct
    10261 tgctcgggca aacaaaccag gtagccggaa tgtgcaagtc aatgatgatg ctgataagac
    10321 gcctaaccag cgtggcgatt cggtttgacg cctgggaaga gaccagggtg caacgatgag
    10381 ggaatttatg gaaccgcgac aaagagtggt gccgtaactg gctaagtgct ctcagcgttg
    10441 tggtgaatgc ggctcagcgc acgcgggtta aggttgagtc tgacagtcga ccttctgtgg
    10501 atacccaccc gtctggtgtg caaccttcgc caggcaccgg gaggcacccg gcaccacaac
    10561 tttatgctgt gtgtagtcct ggcggtacca gtttgtacac ttgcttccgg ctggtaccgc
    10621 tctttttaca aaacagagaa gagcatcacc ggacgacggg ctcataaccc aatccatccg
    10681 ggcggctgcc accgcaggtg ttcttctctg ttttgtggag aaaccaaccg accttgcagg
    10741 gtcgatatga tgaggagcag caaaatggct agcgaacgca gtactgatgt gcaggcattt
    10801 atcggggagc tggacggcgg cgtatttgaa accaaaatcg gcgcagttct cagtgaagtc
    10861 gcttccggtg tgatgaacac gaaaaccaaa ggtaaggtct cactcaacct ggaaatcgaa
    10921 ccatttgatg agaaccgtgt gaaaatcaaa cacaaactct catatgttcg cccgactaac
    10981 cgcgggaaaa tttccgaaga agacaccacc gaaacgccga tgtatgtcaa tcgcggtggt
    11041 cgcctgacta ttctgcagga agaccaggga caattactga ctcttgccgg tgaacctgac
    11101 ggaaaactcc gcgcagcagg tcattaatat cgttcttaat taactgatta tttatctcat
    11161 cactgaatat ctttatatag tgaggactta ttatgtctca gaacttagac gcaaccgcaa
    11221 ttaatcaaat ccatgccctt atttctgctc agggtgttaa tgaaattatc agtaagattg
    11281 gtgccgatgc tgtggcattg cctgagaatt tccgcattca tgatctggaa aaatttaatt
    11341 taaatcgctt ccgtttccgt ggtgcgcttt ccactgccag catcgatgac tttacccgtt
    11401 attctaaaga tcttgcagat gaaggcaccc gctgctttat cgatgctgat aatatgcgtg
    11461 ccgtcagtgt gcttaacctg ggtactattg atgaaccagg tcacgcagat aacaccgcca
    11521 ctctcaaact gaaaaagaca gcaccgttct ctgctctgtt gtctgttaat ggcgagcgta
    11581 actcccagaa gtcactggca gaatggattg aagactgggc cgactacctt gtgggctttg
    11641 atgctaatgg tgacgccatt caggcaacaa aagcggctgc ggcagtccgt aaaatcacaa
    11701 ttgaagcgaa ccagactgct gattttgaag acaatgactt cagcggcaaa cgctccctga
    11761 tggagtctgt cgaagcgaag accaaagaca ttatgccagt ggcatttgaa tttaaatgcg
    11821 ttccgtttga aggcctgaaa gaacgtccat ttaaattacg cctcagcatt atcactggcg
    11881 atcgtcctgt actggttctg cgcattattc agctggaagc ggtgcaggaa gatatggcta
    11941 acgaatttcg tgatctgctt gttgagaaat tcaaagacag caaagtagaa acctttattg
    12001 gtactttcac cgcctgattt cattactgca aatgcccctg cgggggcatt tatggaaacg
    12061 taattaactc aataatcgcc ggatggtgag gacttccttt taccagaatt cagcgcggtg
    12121 cagcgcatat acgtggagaa caaaatgtca tttattaaaa ctttttccgg gaagcatttt
    12181 tattatgaca agataaataa agacgacatc attattaacg atatcgcggt ttccctttca
    12241 aatatctgtc gctttgcagg acatctttca cacttctaca gcgtcgccca acatgcggtg
    12301 ctttgcagcc agctggtacc gcaggaattt gcttttgaag cgttaatgca tgatgcaaca
    12361 gaagcttatt gtcaagacat ccccgcgcca ctgaaacgcc ttcttcctga ctataaacgg
    12421 atggaagaaa aaatagacgc cgtaatccgt gagaaatacg ggttaccccc ggttatgagc
    12481 acgcctgtga aatatgccga tctcatcatg ctggcaaccg aacgccgcga tctcgggctt
    12541 gatgatggct ctttctggcc tgtactggaa ggtatcccag cgacagagat gttcaaagtt
    12601 attccactgt caccaagcca tgcctacggg atgtttatgg aacgttttaa cgagttatcg
    12661 gagttacgca aatgcgcatg aatgttttcg aaatggaagg gtttcttcgc gggaaatgtg
    12721 taccgcgaga tctgaaagtg aacgaaacaa atgctgagta cctggtacgt aaatttactg
    12781 aagcggaggc taagatttcg gcgcttgcgg cggagaatga gctggctcgt aaggcagttc
    12841 aggcattctg cgatgttgtt ggcgacaaca ccgaggttat cgctgaggag gttgggcgag
    12901 atggcgttct ggttattttg gaggccatga aggcaacagg aaatatgcca gccaccgatg
    12961 cttttctggc tgaagtgcgg gcacaggggg ggatatggct cgtaacgcga tgattgattt
    13021 tgttgatggt gaagttgggc caaacaagaa cgttccgggg ctgattagag gcacagagat
    13081 atgcgtaagt attgctgaac agcttcgtaa aggaggcaac cagtgagtgg gattaattat
    13141 caggcgctgc gtgaggcggc acaaaactat cagtcgatgc tggcgtggta tcaggagaaa
    13201 ccagacagtc caaacgctga gcaggattgt gatgcagctt tggctgcgtt taagcgtgaa
    13261 atccgtcatc gtgaagtgga tattatcgct gatttgctgg atgaactgga ggaagcaaaa
    13321 cagcgtatcg acgagcagga gtcccgcaca gtgaagttac cagaaccatt caagctggct
    13381 aaatcatcaa gtggattaac gtactactac gctgatgagg tcaacgccgc actgacttct
    13441 gctggtattc gcatagaagg agagtgagat gaccactatt accaaagagc gactgcaatg
    13501 gctggctaac atttctggcc gcgatgacat tgacgatata gacgtcggtg aaattcgtga
    13561 gctggcgctt atcgcactgg catcgctgga agcagagcct gtggcgtcat gcattattga
    13621 agatggggca tgtgtgttga cgggttcggt gagtatgttg gtcactcgct gcctgatgga
    13681 acgcaccagc tatacgccgc cccgccagtg cctgataagt tgccgcgtga atacataaga
    13741 ggttggcctc ttgcgcatag tgattatgca gaaggatgga acgattgccg tgctgcaatg
    13801 cttaatggag ataaatcatg attgatcgaa ccaaactgga gcacattctc gaatatgcca
    13861 ggcagcagaa atgtattggg caactttgta aaattccacc aggagatatg cttgaaatcg
    13921 tggaaatggc catgcgtaag gctggcaact ctccggtaac tccggctcgc ctgcctggtg
    13981 gtttcaccat tgaggatgcg aaggagttat atgaagacct ggtacgcagc cacataagca
    14041 aggctttaag tggcgaaaag atgaagaaga aggagcgcga tgctgatttg cgctggatcc
    14101 atggggttat agtccaggca gcctggtttg taaaagcatc actggagcag aatgcgctaa
    14161 cgggtaactc tcaggtaatc cctgatgacg gtcgcgaaca gttcgaaaca cttatcaggt
    14221 tccatgcaga tgataaaaac catgaaacat tattgcttcg tgctaacgaa gggatgaatt
    14281 accgggatcc aaatgtggat ttggcgtgga tattctggaa atccagtcga gagcacatat
    14341 tgccgggaaa aagcgacaat aatccagcat taggcgatca ggtgagtgaa ttaactatgt
    14401 tggttaagcg attagccagt tcattaaaaa gcgtcaataa atcaagcaag ctacctgata
    14461 cggcgatgga gtatctgaaa cagaatgggc tgataaacac agagagtatt ttacgatgac
    14521 ctgacctgaa gtgttctcaa atgttggtat tgcgttggcg attgtgctgg ttgtgtactc
    14581 aatttgccgc tgggggtaaa tacatgttct ctctgattca acgtggtcag atatacacgg
    14641 acagagccgg ataccctgtg gtgattactc gcagcactga gcacttagtg ttctttcgac
    14701 gcatggatgg acactccggt cgggtgcgca tcagtgaatt caacaaccat tttgaacata
    14761 ttgaccatca ggaatttcgc cagatacaga cagaactgga gcaggaagct cacctgaaaa
    14821 aattacgtgc catgaaaagg aagtgaagag taaataaagc atttgaacta tgggtccacc
    14881 agcgttatgg cagtcgctat gacctgacgc gagatgttga cggtttctac tgccgtgaag
    14941 tggtgaaacg aatgtttgaa tgtggtgcca ctgccgtgga tgaaagtttt atgaggtaac
    15001 tatggctctc aacattgatt gtgtacctat aacgacatgg tgtcgagata tgggagaaac
    15061 actggatgcc gttaacaaac ggttacaacg tggagtatgg aaggaagggg ttcaggtttt
    15121 aaatgtggaa ggctccaaag aacgatggat tgatttaact gaggtttcaa aatgggcgag
    15181 aaaaaacaaa tatccttatc actcccaaga ggaataacca ttcgtcaata caagaattgc
    15241 tctacattag ttgtaacctt cacatataaa ggggttcttt gcagagaacc cttaccaagg
    15301 ctggaagcta acgtaaaggg gattaagtat gctgaacgac ttctcggaga gataaaaaac
    15361 caaatcagtg ataactcatt cgatttttca aaatactttc caaactccag gaagttggca
    15421 ttatttggaa tggtaaagaa gaataaaagt gtgagagatt acctggaaga gtatttggaa
    15481 atttgcaaaa acagaaagtt atcgccttca acaataaatg gatatgaaaa atgtgtatct
    15541 gcattatcgg aactacataa gctacatgtt gttgacctga caccagcaat attaaaaaaa
    15601 tggatttcaa aaagaacaac aaaacttaag accataagaa acaatctgtc atttttaaga
    15661 agcgcagtag atgaagcggt cactgacgga cttataacga taaatcctgt aaaattggtt
    15721 agtgcaagta gataccatgc agttaaccat acagccaatg ctgatgatta tgaagtggat
    15781 cccttcacgc cagcagaggc aacagctatc tacaacgcct gtatctatca ggaatggaga
    15841 aatctttttt gtttcgcatt caatacaggc cttcgaagct cagaattatg tgcattgcgt
    15901 tggtctgata ttgattttat tgggcatacc gttcacgtac aggttgcttc tgtcgttggt
    15961 gtgataaaag gaaccaaaac aaaagctggt accagaaaag ttgaattaaa caatgaagcc
    16021 ataatggcat tgcaggccca gaaatcattt acgatgatga gaagtgattt tgtattcagt
    16081 gatccaaaaa cacgtaagcc gtgggctaat tcagctgcaa ttcgtaagaa agcctggatc
    16141 ccgaccctta aaaaagctgg agttcgttac cgcaatccat atcagaccag acatacattc
    16201 gctacacggc atatcagcca gggggcaaat ttattctggc ttgccggtca aatgggtcac
    16261 aaaggaccag aaatgctgtt tagacactat ggttcatatt tatcagaata tgatggcaag
    16321 actgcgatct cagaagtaat ctagctgctt aaatatttca aaatatgtac taacgtagga
    16381 cacagaatag actctgactt acacctgaaa tacataaaaa catacaaaaa caataagaaa
    16441 tcttttgttt ttcatatcgt tatgtaattt gggtgtttgg gttcaactcc cgccagctcc
    16501 accaatcatg attggacggt gtaaggacaa caccaacaaa aacaggaagt tagcagtctc
    16561 agcaagacac cgaccagacg gtgaggagac ataaaaggat acgcaaagga gccgcggctc
    16621 ctggtgatat gaaagcccac agatgtgggc tttttcgttg atggtcagaa cgaccagttc
    16681 acaccagcca ccgcgttcca cggggattcc acaccggcac catggctata ccccaccccg
    16741 agatgcccgc ttaacgaact gctgaatgag gctttaatac ctgcctggta tattccacgt
    16801 ctgcccgaca aatcattgac aaaattaccg tcattattca ctttcacccg gttatcatcg
    16861 acaaattctt tgcgcacagc cgccttcagc cacggctcaa cttccatgcc gttccccaga
    16921 cgcatgttgt aactcagcgt tgcgcccagt tcacgatata tactacgggt atcgactgat
    16981 ttcgattcca tgccattgga taaatgatat tcggggttat cagcggtgaa ccccgttaac
    17041 gatgcatacg gcgtcaggtt ccagttacca tcggtaaatc gcatcccggt ttcaatgtga
    17101 ccgcccagcc cgttgctgcg ataactgccg ttggcggctc caccgctgct cattttaccg
    17161 gctacgttac tttcaaagcg gttcagcttc acgataccgt ccagatagaa accactttca
    17221 tgttcccagc tggcatagcc ccccagagaa taactgccca cactgccatg ccctccacga
    17281 tcaaaaaaga tatgtgtatg ggaataaccc ataaaagcgc ccagcgtcgc aatcccctca
    17341 ggaatatcat tacggctatc gatccccacc gtcattccgg tcagcgtctg ctcaaacccg
    17401 gcacccgcat cggtggtgac attattacgg gtgttatacg tcgtccccca gacattattg
    17461 ttgtgtggac tcgctttcat tatgttcaac cgctcgcgaa tactgtttag ctcagcatca
    17521 aagaccaacg gtaatgttgc tgccatattg agtacggctg ccgtagaagg cgtgatgcgt
    17581 ttctccggga cgggtgtcgg cgttggatcg ggagtcgggt ctggtttcgg atccggtttt
    17641 gggtctggtt ttggatccgg cttcggattt gggttggggt ccgggttggg tttgacatca
    17701 ttggtcaggt tccagttgct gttgccatcg cttttcagga catactcata ggtcccaaga
    17761 tcaacgaaac cgccggtatt acccagcgta aacgaagcat cccctccccc tgttttcacc
    17821 agcgtcatcg cgtcgtcaga ctgagggctg acgccggtat cctgaacaaa gattttaaaa
    17881 ttgccagtgg cgttgttgtt gacgaccagt tgatcgcccc gggagcctgc aacgttggta
    17941 tgcaggtaga aattaccgct gccggaaagt tcactggtgg tcagcgtatt gtagatacca
    18001 ttagttgtgg caggcgctgc tgtttgtgct gataaataaa catcaccgtc gttgaggagc
    18061 aaactattta ctctatattc gcagttgctg gtgcctgcac aggaattatt gctgttaagc
    18121 cagacactgc cccggctggc agccgtcagg tcggcaagcg tcgtatcgac accatttccc
    18181 agagttaatg tcgcgctatc ggtaatccgg accgcccctt caagttttac tggcgtaaca
    18241 ttatcccgtg gcgtcattaa cgacaggctt cctgtcgcgc cactgaccga tgcatccgcc
    18301 agcgtacctg cgtagacgat tgccgtcccg ccagcaacct gtagatcttc tgcccgggcc
    18361 agaacctgaa actcatcttt tgaccgaccg agggtatatt gcccaccaga attaaccttt
    18421 gtggcgaagt cctgccccag gttttgcatt gccccaccgt tgccaaccgt ggaatcgcga
    18481 gcttcggtac ctgctaatac caataaatta ccgccatttt ccagcagtac attggtcgct
    18541 aaatatccgg caatggaaaa ggtgccgtac tggtgcgtac cgctgatttc aataccgtta
    18601 gccgtgctcg tctggagagc ggcaccgctg ttctggacga tatctgtcgc tttgccatta
    18661 tcgttgactg tcagcgttcc gccttcattg atcgttgttt ttattgcctc tccgttagct
    18721 gaaactgttt gtattccgcc gtcgttaatt gttgtctcat ttgccacacc ctcgacgatt
    18781 tgttcaccgc cagtgagcgt cgtgccagtc gcagtggctt ttgttttgac gatctcccga
    18841 ccgcccgcat tgacctgtgt tttggcggaa gtggtgtctg actccacggt taacacgccg
    18901 ccatttgtca gcaggatatt gttcgccgca ccctgctcga tgctgaacgc gacgccatca
    18961 gcgcgtgtcc ctgtgacccg cgtcgccctg gtggttgcaa ccaacgcgcc ctgactactc
    19021 tgctgtatcc ccgttgcgct gcctttctcc cgcacatcga gtgtgccgcc gtcattaagc
    19081 accgagtttt cagccagacc gccctcatta actacctgtg aaccgccatt aataatggaa
    19141 ccttccgctg tcccgtttgc cataatttgt tgtaagccag agacgatatc ggtattgatc
    19201 gccttaccat aattctgaac ggtttgtgtg ccgccattga tgtgcgtttt gtctgttgac
    19261 ccaccatcaa caatttgttc accactttca atatttgcat cagtggctaa accatatacc
    19321 gtttgcttgc cacctttgat atttgcttta tcagaagtgg cactggcata tattgtttgg
    19381 gtgcccgcac tattgagtac agtgccaaca tcttttccat aaacatccat tttgccgttg
    19441 gcattaataa tcgtatcaac tgcccgggaa ccagtgacga ctgttaatga gccagcgttt
    19501 tccagcacta catttttagc ttctgaattc ctgatgtaga aagcatcacc ataactgttg
    19561 gttccttcga taagggttcc ggaagtcgtg gaagcaatta atgcgccgcc ggattgttgc
    19621 tcaacatgct tagcctcacc accgtcctga acctccagaa cgccaccatt attaagtctg
    19681 gtgctatctg ttttagcctc cttctggaca atcagcttac cgccagcatc aatggtagta
    19741 tttttcgccg aggttttagc cattaccgtc agttcgccgg tattttccag cacaacataa
    19801 ttagcctcac ctccggtaat agtgaagtga gagagcttat tgtatccttc gatatcagtc
    19861 cctgcgcccg tgttggcaac taaagcactg cccgtctcct ggttaacccc atgtgcaata
    19921 ccgccggtat agacaatcag cgagcctccg gcgctaatat tgctgccagt tgccgtacca
    19981 tctttctcaa caacctgccg gctcccagag gatatatttg tcgtgtcagc tttcccgccg
    20041 ctcttaatat tttgtgttcc gctgttgata ttggtgcccg tggcaatacc ataattatta
    20101 atattctgtg tgccaccatt aattatggta tttgtcgcgt ttccggcaac atccataacg
    20161 ccgccattat ctattcgggt cgcatcagct ttagcattcg ttaaaactga cattgttcct
    20221 ttatctttaa taatcgtctt atttgccgaa ccatatgcgt ttatgtctaa atgaccaccg
    20281 ttttccagca acacattgtc tgccacgtta ttgtggatgg agaatgcccc ttcactattc
    20341 gtaccgctca ccgtcaaatc gtaagtcgtt actttcaaag cggctccatc gtgctgagta
    20401 acatttgttg ccataccacc tctaacatca agcacgccac cggaataaat ttcaataaca
    20461 tccgaggtgc tagtgtaatc aacaatttgc gtaccaccag aatagatctg agtatttttt
    20521 gccgttgact tactatccag agactgagtt ccaccttcaa tcgtcgtgtc cagcgcacgg
    20581 ctctcatata ctctttgctc accgccattt ttaatggtgg ttgtttctac tgtgctctgt
    20641 tcaacatact gtcgaccacc atttatggtt gtgttcgtcg ccagacttcc ttgcgtgatg
    20701 tcctgataac cggacttatt tatcgttgta ccatcaacat gcccctgagt tgttactgtc
    20761 tgccctccac catcaatgat ggttccattc gcagtccccc cacttatgtg tgaattacca
    20821 cccttaattg tcgttccatt gctgatacca cctgtataaa cgtcctgatt gccactctcg
    20881 attattgtac ctgtggaaat acccccgtca tgaattgact gtctgccccc gttaatggtt
    20941 gtattattag cctgccccac aaaattgtta tgacctccta catcttgata tccacccgat
    21001 tcaataagac ttccattaga tatcccgcca taaacattct gctggccatg gttgataata
    21061 tgagtgttat ttgttgtacc tcgttcatct actttttggc tgccatctac agtctcatcg
    21121 tttaccacac caataacatc aggagtgaag gccgccatcc cgggcggggc atatatcaag
    21181 gcagatatca ataaggaaag tactgagcga cgacaataat agggactggt cctgttcata
    21241 aatttcatcc tctgaaaagt gaatactgag tagcgtttaa gcgaccttag ctttgctgca
    21301 acatcagccc acaggcacca gaccagggga ttcatcctga agagacagcg caagtgtatt
    21361 gtgttcaccg ctcatcaaag acatcatgat gaaatgatga tattccgcat aagaatgagg
    21421 cattttttaa acgcagtgcg ctgaagtgtg gttggataaa aaagtcaatc tattcaggaa
    21481 acacgggcgt attcttttct ttcgacaatc aggcagtcgg aaaaataaaa tgatttacat
    21541 aatcgtttct gatgaatatc ttctgctcac ataaaaatca cacaataact ctgagatcgc
    21601 agattgtttt acttttacag cttccgtccc cccattgttg ggcaaatata gattgggcca
    21661 gagcacgaaa gttaatacca cgttcgcaca gctcctccaa cagcacgaca agatgccaca
    21721 tactgcgccc cagtcggttc agtttacaga ctagcagtgc gtcccctacc gataatgtcc
    21781 tgagccgttt gttctgtccg ggcctgctgc cacggttatg cgctgaagtg gtaactctga
    21841 gcacgcagtt cttcagtcag gcgtggtgca ccgtcaccct attattgctg ggtaagatca
    21901 aaaaactttc aagcagctaa ggaaagttga accagacatt agatgaaata tttcaaccaa
    21961 ttacagcacc agttcagaca ccgccaactc atcaaataaa tcaaggggtt acgtgaaagc
    22021 gtagcccctt ttactttggt agtgacagca aaatggatgc agtgtgaaga ataatcccgt
    22081 ttactcaatc aataatacat attatttcaa tctacgttat tatttctttg taaaaatcgc
    22141 catttattta tcattgaaaa ctgcttttag aactttatac aaggggacta gtcacaacag
    22201 gactattctc aacgggctca tcctcagagg aactatcatc aaagtcatca tccatagatg
    22261 aaataccatc gaatggtgcc acgcccgtga ttagttttat tttattatta cgatcagtca
    22321 agactccatt taaaccattt tcgctgacgg attttaataa tttatcataa ctcttattgt
    22381 catcaggcac attaaaaata cacggagtat caacatcaaa caatgacgtt ccccagttca
    22441 catatttaat atcatagtta ctgaagttct atccagaaaa gaagcatccc ttaaaatcca
    22501 atccacctaa attatataaa ccaccctctt ctttttggag agcaatgtta attttggcta
    22561 tctcccggac atcatcgcca tttttgtatt tgaataccgt ttcaagatgt tctccagaca
    22621 gcttgacttc tggaaataat ttgaaattaa accctacatt attatgtgtc aacgtagatg
    22681 aacaaaagat ggaaaaagct tgcagtgctg aattatagct atcg
//
LOCUS       DAZKJR010000081        22192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKJR010000081 DAZKJR010000000
VERSION     DAZKJR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.081"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..2396
                     /gene="polB"
                     /locus_tag="ACHOWS_003136"
     CDS             45..2396
                     /gene="polB"
                     /locus_tag="ACHOWS_003136"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFQ0048672.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGSIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            2560..5466
                     /gene="rapA"
                     /locus_tag="ACHOWS_003137"
     CDS             2560..5466
                     /gene="rapA"
                     /locus_tag="ACHOWS_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFQ0048673.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            5478..6137
                     /gene="rluA"
                     /locus_tag="ACHOWS_003138"
     CDS             5478..6137
                     /gene="rluA"
                     /locus_tag="ACHOWS_003138"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFQ0048674.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6255..7070)
                     /gene="djlA"
                     /locus_tag="ACHOWS_003139"
     CDS             complement(6255..7070)
                     /gene="djlA"
                     /locus_tag="ACHOWS_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFQ0048675.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKLQKGFK"
     gene            7325..9679
                     /gene="lptD"
                     /locus_tag="ACHOWS_003140"
     CDS             7325..9679
                     /gene="lptD"
                     /locus_tag="ACHOWS_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFQ0048676.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            9732..11018
                     /gene="surA"
                     /locus_tag="ACHOWS_003141"
     CDS             9732..11018
                     /gene="surA"
                     /locus_tag="ACHOWS_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFQ0048677.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11018..12007
                     /gene="pdxA"
                     /locus_tag="ACHOWS_003142"
     CDS             11018..12007
                     /gene="pdxA"
                     /locus_tag="ACHOWS_003142"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFQ0048678.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12004..12825
                     /gene="rsmA"
                     /locus_tag="ACHOWS_003143"
     CDS             12004..12825
                     /gene="rsmA"
                     /locus_tag="ACHOWS_003143"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0048679.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            12828..13205
                     /gene="apaG"
                     /locus_tag="ACHOWS_003144"
     CDS             12828..13205
                     /gene="apaG"
                     /locus_tag="ACHOWS_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFQ0048680.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13212..14054
                     /gene="apaH"
                     /locus_tag="ACHOWS_003145"
     CDS             13212..14054
                     /gene="apaH"
                     /locus_tag="ACHOWS_003145"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFQ0048681.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKHYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14132..14611)
                     /gene="folA"
                     /locus_tag="ACHOWS_003146"
     CDS             complement(14132..14611)
                     /gene="folA"
                     /locus_tag="ACHOWS_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFQ0048682.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(14803..16665)
                     /gene="kefC"
                     /locus_tag="ACHOWS_003147"
     CDS             complement(14803..16665)
                     /gene="kefC"
                     /locus_tag="ACHOWS_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFQ0048683.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGAVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(16658..17188)
                     /gene="kefF"
                     /locus_tag="ACHOWS_003148"
     CDS             complement(16658..17188)
                     /gene="kefF"
                     /locus_tag="ACHOWS_003148"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFQ0048684.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(17296..18627)
                     /locus_tag="ACHOWS_003149"
     CDS             complement(17296..18627)
                     /locus_tag="ACHOWS_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0048685.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(18686..18973)
                     /gene="fixX"
                     /locus_tag="ACHOWS_003150"
     CDS             complement(18686..18973)
                     /gene="fixX"
                     /locus_tag="ACHOWS_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFQ0048686.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(18970..20256)
                     /locus_tag="ACHOWS_003151"
     CDS             complement(18970..20256)
                     /locus_tag="ACHOWS_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0048687.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(20307..21248)
                     /locus_tag="ACHOWS_003152"
     CDS             complement(20307..21248)
                     /locus_tag="ACHOWS_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFQ0048688.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(21263..22033)
                     /locus_tag="ACHOWS_003153"
     CDS             complement(21263..22033)
                     /locus_tag="ACHOWS_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFQ0048689.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         5341 a         6156 c         5789 g         4906 t
ORIGIN      
        1 aaaccaggct ataatcaagc ctggtttttt attggatttt cagcgtggcg caggcaggtt
       61 ttatcttaac ccgacactgg cgggataccc cgcaaggaac cgaagtttcc ttttggttag
      121 cgacggacaa cgggccgttg caggttacgc ttgcgccgca agagtccgtg gcgtttattc
      181 ccgccgatca ggttccccgc gcccagcata ttttgcaggg tgagcaaggc tttcgcctga
      241 cgccgctggc gttaaaggat tttcatcgcc agccggtgta tggcctttac tgtcgcgccc
      301 atcgccagtt gatgaattac gaaaagcgcc tgcgtgaagg tggcgttacc gtctacgagg
      361 ccgatgtgcg cccgccagaa cgctatctga tggagcggtt tatcacctca ccggtatggg
      421 tcgagggtga tatacgcaat ggcgctatcg ttaatgcccg tctgaaaccg catcccgact
      481 accgtccgcc gctcaagtgg gtttctattg atattgaaac cacccgccac ggtgagctgt
      541 actgcatcgg cctggaaggt tgcgggcagc gcatcgttta tatgctgggg ccagagaatg
      601 gcgacgcctc cgcgctcgat tttgaactgg agtacgtcgc cagccgcccg cagctactgg
      661 aaaaactcaa cgcctggttt gcgactcacg atcccgatgt gatcatcggc tggaacgtgg
      721 tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg ttaccgtatt ccgctgcggc
      781 tggggcgcga taacagcgag ctggagtggc gcgagcacgg ctttaaaaac ggtgtgtttt
      841 ttgctcaggt taaaggtcgg ctaattatcg acggtatcga ggcactgaaa tccgcgttct
      901 ggaatttctc ttcattctcg ctggaaaccg tcgctcagga gttattaggc gaaggaaaat
      961 ctatcgataa cccgtgggat cgaatggacg aaattgaccg ccgtttcgcc gaagataagc
     1021 ctgcgctggc aacttataac ctgaaagatt gcgagctggt gacgcagatc ttccacaaaa
     1081 ctgaaatcat gccgtttttg ctcgaacggg cgacggtgaa cggcctgccg gtagatcgac
     1141 acggcggttc ggtggcagcg tttggtcatc tctattttcc ccgtatgcat cgcgctggtt
     1201 atgtcgcgcc taatctcggc gaagtgccgc cgcacgccag ccctggcggc tacgtgatgg
     1261 attcgcgacc tgggctttat gattcggtgc tggtgctgga ctacaaaagc ctgtacccgt
     1321 cgatcatccg cacctttctg attgatcccg tcgggctggt ggaaggcatg gcgcagcctg
     1381 atccagagca cagcaccgaa ggctttctcg atgcctggtt ctcgcgagaa aaacattgcc
     1441 tgccggagat tgtgactaac atctggcacg ggcgcgatga agccaaacgt cagggtaata
     1501 agccgctttc gcaggcgctg aagatcatta tgaatgcctt ttatggcgtg cttggcacca
     1561 ccgcctgccg cttcttcgat ccgcgcctgg cgtcgtcgat cactatgcgt ggtcatcaga
     1621 tcatgcggca aaccaaagcg ttgattgaag cgcagggcta cgacgtgatc tacggcgata
     1681 ccgactcaac gtttgtctgg ttgaaaggcg cacattcgga agaagaagcg gcgaaaattg
     1741 gtcgtgcact ggtgcagcac gttaacgcct ggtgggcaga aacgctgcaa aaacaacggc
     1801 taaccagcgc actggaactg gagtatgaaa cccatttctg ccgttttctg atgccaacca
     1861 ttcgcggagc cgataccggc agcaaaaagc gctacgccgg actgattcag gagggcgaca
     1921 aacagcggat ggtatttaaa gggctggaaa ccgtgcgcac cgactggacg ccgctggccc
     1981 agcagtttca gcaggagctg tacctgcgta tcttccgcaa cgagccatat caggaatacg
     2041 tgcgcgaaac catcgacaag ctgatggcgg gcgaactgga tgcgcggctg gtttaccgta
     2101 aacgccttcg ccgtccgctg agcgaatatc agcgtaatgt tccgcctcat gtacgcgccg
     2161 ctcgccttgc cgatgaagaa aaccaaaagc gcggtcgccc cttgcaatat cagaaccgcg
     2221 gctccattaa gtacgtatgg accaccaacg gcccggagcc gctggactac caacgttcac
     2281 cgctggatta cgaacactat ctgacccgcc agctacaacc cgtggcggag ggaatactcc
     2341 cttttattga ggataatttt gctacactta tgaccgggca acttgggcta ttttgagcaa
     2401 aaaaagagtt cgccagatac cattttgatg cgtgacgaat gctttgccat ccagtaccat
     2461 agcgcccttt ccattcctgg acctgaataa caccactacc tcataagcac ggtagcgggt
     2521 ggttattgcc tgcaattaaa gatatagagc cgaacacata tgccttttac acttggtcaa
     2581 cgctggatca gcgatacaga aagcgaattg ggacttggaa ccgttgtcgc ggtggatgcg
     2641 cgaactgtca ctttactttt cccatctact ggtgaaaacc gtctgtacgc acgcagtgat
     2701 tcccccgtga cccgcgtgat gttcaaccct ggtgatacta ttaccagcca tgacggctgg
     2761 cagatgcaag tcgaagaagt aaaagaagaa aatggcttgc tgacctatat cggtactcgc
     2821 ctggatactg aagagtccgg cgtagccctg cgtgaagttt tccttgatag caaactggtg
     2881 ttcagcaaac cgcaggaccg tctgtttgcc gggcagattg accgtatgga ccgctttgcg
     2941 ctgcgttatc gcgcgcgtaa gtattccagc gaacagttcc gtatgccgta cagcggcctg
     3001 cgcggtcagc gtaccagcct gatcccgcac cagctcaaca tcgctcatga tgttggccgc
     3061 cgccacgcgc cgcgcgtcct gctggctgac gaagtgggtt tagggaaaac cattgaagcc
     3121 gggatgatcc tgcatcagca actgctctct ggcgctgctg aacgtgtgct gattatcgtc
     3181 ccggaaacct tacagcatca gtggctggta gaaatgctgc gccgtttcaa cctgcgcttt
     3241 gctctgtttg atgatgagcg ttatgccgaa gctcagcacg atgcttacaa cccgttcgat
     3301 accgaacagc tggtgatttg ctcgctggat tttgctcgtc gtagcaaaca gcgtctggaa
     3361 catctctgtg aagccgaatg ggatctgctg gtggtcgatg aagcccatca cctggtgtgg
     3421 agcgaagatg cgccgagccg cgaatatcag gccattgaac aactggcaga gcacgtgccg
     3481 ggcgttctgc tgctgaccgc aaccccggaa cagctgggga tggaaagcca cttcgcccgt
     3541 ctgcgtctgc tggacccgaa ccgtttccac gattttgcgc aattcgttga agagcagaaa
     3601 aattatcgtc cggttgcgga cgccgttgcc atgctgctgg caggtaacaa actgagcaat
     3661 gatgaactga acatgctcgg tgagatgatc ggcgagcagg atatcgagcc gctgttgcaa
     3721 gcagcaaaca gcgacagcga agatgcccag agcgcccgtc aggaactggt ttcgatgctg
     3781 atggatcgcc acggcaccag ccgcgtgctg ttccgtaaca cgcgtaacgg tgtgaaaggc
     3841 ttcccgaaac gcgagctgca tactattaag ctgccactgc cgacgcagta tcagaccgct
     3901 attaaagtct ccggcattat gggcgcacgt aaaagtgcgg aagatcgcgc tcgcgatatg
     3961 ctctacccgg agcgtattta tcaggaattt gaaggcgata acgccacctg gtggaacttc
     4021 gatccgcgcg ttgaatggct gatgggctac ctgaccagcc atcgctctca gaaagtgctg
     4081 gtgatctgcg ccaaagctgc cactgcgctg caactggagc aagtactgcg cgaacgtgaa
     4141 ggtatccgcg ctgctgtgtt ccacgaaggc atgtcgatta tcgaacgtga ccgcgctgcc
     4201 gcctggtttg ctgaagaaga caccggtgcg caggtactgc tgtgctcgga aatcggttct
     4261 gaaggacgta acttccagtt cgccagccac atggtgatgt ttgacctgcc attcaacccg
     4321 gatctgctgg agcagcgtat tggtcgtctg gatcgtatcg gtcaggcgca cgatattcag
     4381 atccatgtgc cttatctgga aaaaactgct cagtcggtgc tggtgcgctg gtatcacgaa
     4441 ggtctggatg cattcgagca cacctgcccg accggacgca ctatttacga tagtgtatac
     4501 aacgatctga ttaactatct ggcttcaccg gatcaaaccg aaggctttga cgatctgatc
     4561 aaaaactgcc gcgagcaaca tgaagcgctg aaagcacagc tggaacaagg tcgtgaccgc
     4621 ctgctggaaa tccattccaa cggtggcgaa aaagcccagg cactggcaga aagcattgaa
     4681 gagcaggatg acgataccaa cctgatcgcc ttcgccatga acctgttcga tattatcggt
     4741 atcaatcagg acgatcgcgg cgacaacatg atcgtgctga cgccgtccga tcatatgctg
     4801 gtgccggact tccctggcct gtcggaagat ggcatcacca tcaccttcga ccgtgaagtg
     4861 gcgctggcgc gtgaagatgc acagtttatt acctgggagc atccgctgat ccgcaacggt
     4921 ctggatctga tcctttctgg cgataccggt agcagcacga tttcactgtt gaaaaacaaa
     4981 gcgttgccgg taggtacgct gttggtggaa ctgatttatg tggttgaagc ccaggctccg
     5041 aagcagttgc agctcaaccg cttcctgcca ccgacgccgg tacgtatgct gctggataaa
     5101 aacggcaaca acctggcggc gcaggtagag tttgaaacct ttaaccgcca gcttaacgcg
     5161 gttaaccgtc acaccggcag caagctggtt aacgccgtgc agcaggatgt tcacgctatc
     5221 cttcagctgg gggaagcgca gatcgagaaa tctgcccgtg cattgattga tgccgcacgt
     5281 aacgaagccg acgaaaaact gtctgccgag ctgtctcgtc tggaagcgct gcgtgcagtt
     5341 aacccgaaca ttcgtgacga cgaactgacc gccattgaga gcaaccgtca gcaggtaatg
     5401 gaaagcctgg atcaggcagg ctggcgtctg gatgccctgc gtttgatcgt tgtgacgcat
     5461 cagtaacgga gccgaagatg gggatggaaa actacaatcc gccgcaggaa ccctggctgg
     5521 ttgtcctgta tcaggatgac catattatgg tggtcaacaa gccgagcggt ttgttgtctg
     5581 tgccgggtcg tctggaagag cacaaagaca gcgtgatgac gcgcattcag cgtgattatc
     5641 cgcaggcaga atcggtgcat cgtctggata tggctaccag cggcgtgatt gtagtggcgc
     5701 tgaccaaagc cgcggagcgg gagttaaaac gccagttccg cgagcgcgag cctaaaaaac
     5761 agtatgtggc ccgcgtctgg gggcatccat cccctgcgga aggtctggtg gatctgccgc
     5821 tgatttgcga ctggccaaac cgcccgaaac agaaagtctg ttacgaaaca ggtaaacctg
     5881 cgcagacgga gtatgaagtg gtggaatatg cggcggataa cacggcaaga gtggtgttaa
     5941 aaccgattac cgggcgttcg catcagctgc gtgtgcatat gctggcgctg ggtcatccga
     6001 ttctcggcga tcgtttttat gcatcaccag aagcgagagc aatggcacca cgtttgttgc
     6061 tacatgcaga gatgctgacg attacccatc cggcgtatgg caatagtatg acgtttaaag
     6121 caccagcgga tttttaatag tcacgctggc gaagttcggt aggccggaga aggcgttcac
     6181 gccgcatccg gcaattttgc gccgattgcc tgatgcgacg cgggccgcgt cttatcaggc
     6241 ctacacgttg aaacttattt aaaccctttc tgcagcttaa tcaattcata ggcctgctga
     6301 atttcctgcg ctttttgctt cgccatctcc atcatctcag gcggcaaacc tttcgccacc
     6361 agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat ggtggtcgca
     6421 tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgttgg gccacgctgc
     6481 gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa ctgtgcaccg
     6541 ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa ttcttcagca
     6601 atgacataca gcacagcccg ttcattcggg tgcagtgaac catcagcaaa cgccgcctga
     6661 atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac actgcgaaac
     6721 tggcgcatct tttcgcgcag cgggtaattg tctgattttc ccacccggaa cgcgttttgc
     6781 gccgcagtac gggaagcgcc atgaaggttc attcggtcca tcaactggct ggcgatatga
     6841 atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac ttcaaaagtg
     6901 gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat tttacggcta
     6961 cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca aaagccgccg
     7021 cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat atattcccca
     7081 aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt accgttttcc
     7141 ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg tcattcaatg
     7201 ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag agatgacgag
     7261 tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac aataaaatca
     7321 acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg ccctttatag
     7381 tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc caagctatga
     7441 ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg ctgaccacgc
     7501 gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca tgcagggtaa
     7561 cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcgccag gacaaccaga
     7621 gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc aggtgatcct
     7681 caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct gggagggtga
     7741 ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac aacgtggcga
     7801 aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg gttctgacac
     7861 ctggagcgtg gtaggcagcg aaattattca tgaccgcgaa gaacaagttg cggagatctg
     7921 gaacgcccgc tttaaggtgg gtccggtacc gatcttttat agcccctatt tgcagttgcc
     7981 ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca ccaccaccaa
     8041 ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg atgccaccat
     8101 cacgccgcat tatatgcatc gtcgtggcaa tatcatgtgg gagaacgaat tccgctacct
     8161 ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata aagtctatga
     8221 agatgaacac ccgaacgatg acagttcacg tcgttggtta ttctactgga accactccgg
     8281 ggtcatggat caggtgtggc gtttcaacgt cgactacacc aaggtcagcg atcctagcta
     8341 cttcaatgat tttgataaca agtacggttc cagtactgac ggctacgcaa cgcaaaaatt
     8401 cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt
     8461 cttcagcgag cagaacacca gtagctactc ggcagagccg cagttagacg ttaactacta
     8521 ccagaatgat gttggtccgt ttgatacacg catttacggc caggcagtgc actttgttaa
     8581 caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca tcaatttgcc
     8641 gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa cccattatca
     8701 gcaaactaat cttgactggt ataactccag aaacacaacc aagctggacg aatccgttaa
     8761 ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg atatggaaat
     8821 gctggcgccg ggttataccc aaacgctgga accgcgcgcg cagtatttat acgtgccgta
     8881 tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg actactctgg
     8941 cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta accaggtgac
     9001 gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta atatttccgt
     9061 tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa catgggagaa
     9121 tgacgacaaa acgggctcac tggtgtgggc aggcgatact tactggcgta tctcagagcg
     9181 ttggggattg cgtggcggaa ttcagtacga tacacgtctg gataacgtag cgaccagtaa
     9241 ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt accgttacgc
     9301 cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg agcaatataa
     9361 gaatggtatt tcgcaggtag gtgccgtcgc cagctggcca attgccgatc gttggtcaat
     9421 tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta tgttaggtgt
     9481 gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc tgaacggttg
     9541 ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca tcgaacttcg
     9601 cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga acattctgcc
     9661 gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg gttaattgaa
     9721 atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata
     9781 ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg
     9841 tgctggaaag cgacgttgat ggattaatgc aatcggtaaa actgaacgct gctcaggcaa
     9901 ggcagcagct tcctgatgac gcgacgctgc gccaccaaat catggaacgt ttgatcatgg
     9961 atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat gagcagctgg
    10021 atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag atgcgcagcc
    10081 gtctggctta cgatggtctg aactacaaca cctatcgtaa ccagatccgc aaagagatga
    10141 ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag
    10201 tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag ctgaacctga
    10261 gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaagtgaac gaagcggaaa
    10321 gccaggcgcg tgccattgtc gatcaggcgc gtaacggcgc tgatttcggt aagctggcga
    10381 ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg ggccgtattc
    10441 aggagttgcc ggggatcttc gcccaggcat taagcaccgc gaagaaaggc gacattgttg
    10501 gcccgattcg ttccggcgtt ggcttccata ttctgaaagt taacgacctg cgcggcgaaa
    10561 gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga
    10621 tcatgactga cgaacaggcc cgcgtgaaac tggaacagat tgctgccgat atcaagagtg
    10681 gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc tctgctaacc
    10741 agggcggtga tctcggttgg gctacaccag atattttcga tccggccttc cgtgacgccc
    10801 tgacccgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc ggctggcatt
    10861 taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag aaagatcgtg
    10921 cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg atgcaggaac
    10981 aacgtgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt
    11041 gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc ttgcacagcg
    11101 tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg accgggcagc
    11161 gatgctcggt ttgccgctca ccctccgtcc ttattccccc aactcccctg cacaaccgca
    11221 aactgcgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca ctgcggggca
    11281 gttagcggtt gaaaatgggc attacgtagt ggagacgctg gcgcgagcgt gtgatggctg
    11341 tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg tcattaacga
    11401 cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa
    11461 agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc
    11521 gctgcgcgat atcgcagatg ctatcacccc tgcgcttttg cacgaagtga ttgctatttt
    11581 gcatcacgat ttgcggacca aatttggtat tgccgaaccg cgcattctgg tctgcgggct
    11641 gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca ccattattcc
    11701 ggtgctcgac aagctgcggg cgcaggggat gaaactcaac gggccgctgc ctgccgatac
    11761 cctgtttcag ccgaaatatc ttgataacgc cgacgcggtg ctggcgatgt accacgatca
    11821 gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta cgctgggcct
    11881 gccctttatt cgcacatcag tggaccacgg taccgcgctt gaactggcgg gacgtggcaa
    11941 agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga ttgttaacac
    12001 ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg gcaaaacttt
    12061 ctcaacgatc agttcgtgat cgacagcatt gtttctgcca ttaacccgca gaagggccag
    12121 gcgatggtcg aaatcggccc cggtctggcg gcattgaccg aaccggtcgg cgaacgtctg
    12181 gataagctca cggtcatcga acttgaccgc gatctggcgg cacgtctgca aacgcatcca
    12241 ttcttaggcc cgaaactgac tatttatcag caggatgcga tgacctttaa ctttggtgaa
    12301 ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta taacatctcc
    12361 acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat gcactttatg
    12421 ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaggc gtatggtcga
    12481 ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tgctggaagt accgccgtca
    12541 gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg
    12601 atgcctcacc cggttaaaga tgttcgcgtg ttgagccgca tcaccaccga agcctttaac
    12661 cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga ggtgttaacg
    12721 ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca atattgccag
    12781 atggcgaact atctggcgga gaacgcgcct ttgcaggaga gttaacgatg atcaattcgc
    12841 cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca cctgataatg
    12901 aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg ccagtgcagt
    12961 tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg
    13021 aaggagtggt tggcgtccag ccacttatcg cacctggcga agagtaccag tacaccagcg
    13081 gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa
    13141 atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc acactcattc
    13201 attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac gatgaactga
    13261 tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg ctgacgggcg
    13321 atctggtcgc gcgcggcccg ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg
    13381 acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcggta tttgccggga
    13441 tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg gatgccgacg
    13501 agctgcttaa ctggctgcgt cgccagcctc tgctgcaaat cgacgaagag aaaaagctgg
    13561 tgatggccca cgccgggatc acgccacagt gggatctaca gaccgccaaa gagtgcgcgc
    13621 gcgatgtaga agcggtgctg tcgagcgact cctacccctt cttccttgat gccatgtacg
    13681 gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg cggtttatca
    13741 ccaacgcctt tacccgtatg cgtttttgct tcccgaacgg tcaactggat atgtacagca
    13801 aagaatcgcc ggaagaggcc cctgccccac tgaaaccgtg gtttgcgatt cctggccctg
    13861 tcgctgaaga gtacagcatc gcctttggtc actgggcatc gctggaaggc aaaggtacgc
    13921 cggaaggtat ttacgcgctg gatactggct gctgctgggg tggtacatta acctgcctgc
    13981 gctgggaaga taaacactat tttgtccagc cgtcgaaccg gcataaggat ttgggcgaag
    14041 cggcggcgtc ttaaacacag cctgatacag gaaggccgga taagacgcga ccggcgtcgc
    14101 atccggcact agccgtaaat tctatacaaa cttaccgccg ctccagaatc tcaaagcaat
    14161 agctgtgaga gttctgcgca tcagcgtcgt ggaattcgct gaataccgat tcccagtcat
    14221 ccggctcgta atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat
    14281 acagtttttg cgctttgggc aggaactgct cataaacgcg accgccgcca atcaccatga
    14341 tttctggtac gtcaccacac gccgcaatgg cttcatccac cgacttcacc cacgttacgc
    14401 gatcgtccgt acccggttga ctgctgagga taatattttt gcgtcctggc aacggacgac
    14461 cgatggattc ccaggtatgg cggcccataa tcacgggttt atttaaagtg ttgcgtttaa
    14521 accaggcgag atcggcaggc aggttccacg gcatggcgtt ttccatgccg ataacacgat
    14581 ctaccgctaa cgccgcaatc agactgatca ttgagatttc ccgataaaaa aaattgtcgc
    14641 cactatacgt aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg aggaaaattt
    14701 tatattctgc tggcgagtcc acgctctccc actggactcg ccgcattaca atgaaataaa
    14761 aacaattagt tagctgtaaa gtgtgattta cgtcactctt tattaggatg agggtttcgt
    14821 ttccggttca tccgccatgt tgccggtatg tttaccttct tcggttccct gccagccatg
    14881 tcgttgaatt aatgacagat gttcgcggtc ctcggtaatg atctcactta acatcgcgct
    14941 ggtgcgttta tagaccgccg cgcgggcttt ggtgtcgttc tcaaccattg ccatctcttc
    15001 caccatctga atattaaagc ggcggaacac atcggcgcgt tctcgcgctt catacggccc
    15061 cagacctaaa ctttccagtg ccagacgccc ggttttcagc gcaccttcaa aggtttcacg
    15121 ctccggcttt tcaacgcctg cctgacgcaa acgaatgtag tggtcgacat cgcgggcgcg
    15181 ggcaataatc tgcaaatgcg ggaaatgttc tttcaccatc tccgtcagtt gcaggttggt
    15241 ttgcggatcg tcgatagcgt taatcagcac ttccgctttc gccgctccgg cagattccag
    15301 taaatccatc cgcgtggcat cgccataaaa cactttcata ccaaatttac gcaaggtttc
    15361 gatatggtcc ggatcgtgat cgagcaccac cattttcacc ccgctggaga gcagtaaacg
    15421 tccggtgatc tgcccaaaac gaccgaatcc ggcgatgatt acccgcggct gttcttcgtc
    15481 gatctcatcg gcttcacgcg cctcttcact agaagattgc tcaaggcgat tgaggatcac
    15541 cagcagaatc ggcgttgccg ccatcgacag cgccaccgcc agggtcagcg atttcgccca
    15601 ctccggctcc agcacattcg ccatctgcgc cgcaccaaat actacaaagg caaactcact
    15661 accctgccct aatagcaccg caaaccagcg acgctgttta tttggcacct gcaacggccg
    15721 ggcaatcagc cacagcatgg cgattttgat gatgaggaaa ccgagcagca aaatgacaat
    15781 gcgcaacgga ttttcaagca gcgtgccaaa gtctatcgac atgccaacgc cgatgaaaaa
    15841 cagccccagc agcaaacctt taaatggttc gatatcgctt tccagcgcat gacggtattc
    15901 cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca agccgacctc
    15961 ttccagcagc aaaccaaagc caaacacgag gaataacgcc acggcactaa acacttcccg
    16021 caagccagag cgggcaacaa aacgcagtgc cggacgcgtg acatagcgcc ccagcaatac
    16081 caccagcacc agcgcacccg ccacttttaa cgccgagaga gcaaatgcgc ccatcgtcgt
    16141 cgaggcactg ctcgccgcca gtagcgggat catcgccacc agcgggatcg ccgcgatatc
    16201 ctggaacagc agcaccgcaa aggcactgcg acccatttgc gtcaccatca gattgcgttc
    16261 attcatcgcc tgcatggcaa tcgccgtaga ggagagcgcc agcgtcatgc cgatcaattc
    16321 cgcgacctgc cagcgcagcc caagcaacat acagaacagc cccagcaggc cgccgcaaat
    16381 caccatctgc aatgcgccgc cgccgaacac cgccgcacgc agcttccaca gcctttgtgg
    16441 atcgagttcg aggccgataa taaacagcat cagcaccacg ccaatctcgg caaagtgcag
    16501 aatagattcg gcatcggtca ccagtcgcag cccccacggg ccaataatgc agccggcgat
    16561 caggtagcca agtaccgctc ccagaccgag acgtaccgca atgggaacaa tcagcgccgc
    16621 cgaaccgaga taaatcagcg cctgaataag cgtatggcta tccatgatgt gcctcctgcc
    16681 attccagcag acgttgctta tagtgccgcg cctgcccttc gagggtttcg tcgtcacaaa
    16741 taaaggtgca gtgcatggca aacggtggca gccagttcag cccgcagtag attgccgtcg
    16801 cctgtagcgg ctgtgacagc acatcaaagc ccggatgcgc accaatctca aaatggcttt
    16861 ccccaccgcc ggtcgtcacc gcccacagta aatgtttgcc atgcagcgcc gtgccgccgt
    16921 gaccgtaagc ccagccgtgc gagaagactt tatcgatcca aagtttaaga agcggaggaa
    16981 tgctgtacca ctgcatcggg tgctgccaga cgatcagatc ggcgcgagac agcgcctcct
    17041 gctcggcggc aatatcgata ttgaagtcag gatagagttg ataaagagag cgaatttcga
    17101 cgccttccag cgtccttgcc tgttcaagca tccgtttatt cgcatgggaa tgatgcggat
    17161 acggatgcgc ataaattata agaatcatag gtagcccgcg tgattacgcc tttgttttcc
    17221 cacaaagtgt agtcatatat tcacgcggct tatagtcaat aatattgatt cagaaaagag
    17281 agaaatctga acaaattacc ccattctctg cccgcggatc gtcatattgc tggtctgcgc
    17341 cagtgacatc cctcgagtct ccggggcaaa cgctacggaa atcaacaagc caaacagcga
    17401 gatacccgcc cccattagca tcgtgttact gataccgtaa ttattgataa agatcggcag
    17461 tgcccaggtc gaaacaatag tgccaatacg gcttaaggac ataatcacgc ccacggcaga
    17521 ggcgcggata tccgtcggga agagttcatt aggatagagc cactgcaaat tacccggccc
    17581 gccagagaaa aaggcataca ccgcaaatgc catcactacc agccagatcc ccatatccgg
    17641 gatcagcccc aaaaccgcca gcgccagcgt catcatggca aagctgccaa tcaacaatgg
    17701 acgccgtccg gcagtgttta gccacagcat cggtggaata cagccgagca taaagaacag
    17761 gctaatcacc acgttcccca gtgccgcgtt tttgccaaca cccaatccca acaaaccaac
    17821 gatttgcggg ccaaaggtgt agatggcgaa catcgggatc acctggcagg tccagatggc
    17881 ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg
    17941 ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac agtttgatca tcatctcttc
    18001 gcactctttt actcgccctt tgcgtaacaa ccagcgggga gattcaggca gttcgaatcg
    18061 accaatcaaa atcaacaaac aggggatcgc tgcgctaccc agcatccagc gccaaccacc
    18121 ttccacatca taaagccagt aaccgaccag atcggcgcag gtcgcgccga cataccacat
    18181 cgcggcaata aagctgatgg aaaacgcccg ctgacgggta ctggagaact cagtgatcat
    18241 cgaagtggcg atgggataat ctgcaccgat gacaatgccg atgagtaccc gcatcactaa
    18301 cagttcgacg ggggatgaga caaacatcgt cgccaccgat atcacgccga tggcgatgat
    18361 atcaatgagg aacattttgc gccgtccgac tttatcggag atatagccaa acagcgatgt
    18421 gccaacgaac agcccggcga gcgttcccgc gcccagcaag ccaatccagt cagcgtccag
    18481 tttcagcgcc ggagtcagtt gctccagcgc cacgccaatc attaccagta cataaccatc
    18541 cagaaacgga ccaccgcttc cccacagcaa aatgcggcgg tgaatagagg agaatttaag
    18601 atcatcaaag tttctggacg gttgcatggg ttattcctgt ttttttgcaa tgagagtcag
    18661 tacggcatta cgtatcaaac caacatcagc cgtaacggaa ctccacacca aaggtgccgc
    18721 gcgggtattc ccactgttcc agcgccgacc ccagccccag aatgcgacag gtgccgcact
    18781 ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc ctgcttcttg tacagacctg
    18841 cggggcacgc tttcaccagc agctccaggg cctgtttatc ggcatcggcc ttcacaacga
    18901 tgtgcggatg ctcttcatcg acattgaatt tattgacgcc cagtttgacg tccacattga
    18961 cgggagaagt cataaaacgg tcactccttt catgccatcc ttgatcagat tgatgaagcc
    19021 cactttcttg ccgtggcgga ggattttctt gcgcatcagt tccggcgcac tgccgtcaat
    19081 ggtgaacagg tcacgcgcca cgcccaccgc cagttccggg tagccgctaa acatgcgtgg
    19141 gttatcaagg aacgccggta gtttctggta catgcgcata tcgcgcagcg ggccactctc
    19201 aagatgctga cgatattccg cgagtttttg cttactgaaa tcgtcgcttt tcatcgctga
    19261 aagcacagtt tttgctgctg cttccccggc ggcaatcgcc agatccatac cgcgaatggt
    19321 aaaaccgagg ttcatacaca ttccggcagc atcaccggca atcaatacgc cgtcaccaac
    19381 caactccggc agcatgttga tacctgcttc cggcactacg tgagcggaat attccaccag
    19441 cttgccgccc gcgatcagcg gtgcaacggc cggatgttgt ttgaaatctt ccagcatttg
    19501 cggcaccgat ttttttgcgt catgcagatg atgcaaacca caaaccagcc ccagcgacag
    19561 ggtgttttca ttggtataaa gaaagccgcc gcccatcagg ccatcagtgg gggaacccgc
    19621 aaacaggcaa gccgcaccct gattaccctg caactgaaaa cggtcttcga taaccgactt
    19681 cggtaattcg atcagttcct tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc
    19741 catccccagc ttttcggcaa ggatggagtt caccccgtca gcaaggatca ccgttttcgc
    19801 ttcaattaca tcgccatcgg cttctacgcc gacaactttg ccatcgcgct gtacgaggtt
    19861 atcgacacga atcccggtaa ttaactgcgc acccgcttct tcggcctgct ccatcagcca
    19921 ggcatcaaat ttactgcgca aaacggagta agaacgctgg gatggtgagg tttcgtcacc
    19981 gttgcagtag tccatagtca tcgcagactt ttccgtcata aacgcgagtt tttcatgggt
    20041 gatcaggcgt tctacggggg cggagtcagc gaaaccagga ataatgtgtt ccagactgtg
    20101 ggcatagaga cgcccgccgg tgacgttctt ggcaccagcg gaattgcctc gctcgataac
    20161 taacacttgc gccccttcgc gggcgagcac cagtgcggca accgaaccgg caagccctgc
    20221 accgacgatg atggcgtcaa agatatcttc ggacataaga gctccctgtc agcggcaggg
    20281 ccaaaatgca gccctgccag agtggatcaa cgcgctaaag ctgcggtcag tgccggaagg
    20341 atcttcacgg cgtcgccaac aatgccgtaa tccgcgtact ggaagatcgg cgcattttta
    20401 tctttgttga tggcgaaaat ggtttgcgac gcattagcgc caaccatgtg ctggatctgc
    20461 ccggagatcc ccaccgccag gtacagttca ggtttcagca tcaggttgga gataccgaca
    20521 tagcgttcgt gctccatcca tttttcgttt tccgccaccg gacgagaaca ggccaactcc
    20581 gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt tctctttgct gccaataccg
    20641 cgaccaacgc tgaccaccag gcgggctttg tcgagatcga cgctgttgct ctgacgcgcc
    20701 tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc actccacggt gtgcgtttcg
    20761 ccagtgcgtg acgcgtctgg ctgagccaca tcgaacgtgc cgctgctgat ggtcagaacc
    20821 gcatacggcg tggcaatgcg ttcttcgcca atcgccagac cgccgtaaac catgtgtttc
    20881 actgtcgctt taccgtcctg cacgctaaca gcgctggcat cgttagacac cgccgcgtta
    20941 aggcggtaac ccagtttcgc cgccagtaat ttgccgcgac gggtgtttgg caacagcacc
    21001 agaccgtctg cgccgtgctg gcgaatagtg tcagccatga caccggcgta atcttcgatc
    21061 atccgctcgt ccggtttgcc gcttaatttc cagacatgat tagcgccgag ctggattgcc
    21121 tgtgcgccgt cggcatcatt gaggacgaac gtgttgattt gattagctaa agcctgcgca
    21181 ccgttcatca gttccggcag acgagaaggg gtatcgctga atacccagac ttgagaaaac
    21241 gtgttcatag catcccctgt aattaaatga ctttgcgaag attttcagca aatgcggcga
    21301 tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg ctcgcgctgt ttcggcgcgg
    21361 caacctgttg ttctgaccag gctgcctctg cgttaaaacc gatatccgcc gccgaccata
    21421 cctggactgg ctttttcgcc gcgccgagaa tggctttcat cgaaggaatt tgtggggagt
    21481 tgatatcagt ggaaacagca acaaccgcag gcagcggaat gcttaaggtt tctgtttcat
    21541 cttccagttc gcgctcaacg gtgagggtat ctgcagtcag ggagataatt ttgctgacgc
    21601 cgttaactgc cggaatatta aggatttcgc ctaccagcaa accaacctgc tgggcataaa
    21661 ggtcggaaga accatcgcca cagaggatca aatcaaagcc tgctttctga gcggctgcag
    21721 ccagtacgct cgccgtttgt tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa
    21781 tcagttcatc cggaccgcgc gatagcacat ctttacgccc tttggcgttg gtcagggctt
    21841 taccgcccac acttaaggcc gtcacctgcg cctctgctgc ctgttgcttt agctggcaag
    21901 ccgcttcaat agcgttgaga tcgtattggc ttattttggc atcggctttg ctgaagtcta
    21961 acgaaccatc agcattattg accgcaatat cctgttcatc aggcacgcac ttatagcaag
    22021 taataatctt cattgcatct ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt
    22081 taattacaga tatcacgccc tttaatttgg aaataaaact ttaatcacca atattgaaaa
    22141 tgtcacacgc agataaatcc aactttcaat attgttaagc tcctcaccaa ta
//
LOCUS       DAZKJR010000082        22141 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKJR010000082 DAZKJR010000000
VERSION     DAZKJR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.082"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..1821
                     /gene="ygeV"
                     /locus_tag="ACHOWS_003154"
     CDS             43..1821
                     /gene="ygeV"
                     /locus_tag="ACHOWS_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0048690.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFISEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1861..2340)
                     /gene="xdhC"
                     /locus_tag="ACHOWS_003155"
     CDS             complement(1861..2340)
                     /gene="xdhC"
                     /locus_tag="ACHOWS_003155"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFQ0048691.1"
                     /translation="MNHSETITIECTINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITGIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2337..3215)
                     /gene="xdhB"
                     /locus_tag="ACHOWS_003156"
     CDS             complement(2337..3215)
                     /gene="xdhB"
                     /locus_tag="ACHOWS_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFQ0048692.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCQLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3226..5523)
                     /gene="xdhA"
                     /locus_tag="ACHOWS_003157"
     CDS             complement(3226..5523)
                     /gene="xdhA"
                     /locus_tag="ACHOWS_003157"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFQ0048693.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAISINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAAPIHNGGN
                     LLKQSSMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5939..6691
                     /gene="actS"
                     /locus_tag="ACHOWS_003158"
     CDS             5939..6691
                     /gene="actS"
                     /locus_tag="ACHOWS_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFQ0048694.1"
                     /translation="MSAGRLNKKSLGIVMLLSIGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKNSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6770..6843
                     /locus_tag="ACHOWS_003159"
     tRNA            6770..6843
                     /locus_tag="ACHOWS_003159"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6802..6804,aa:Gly,seq:ccc)
     gene            7008..8222
                     /locus_tag="ACHOWS_003160"
     CDS             7008..8222
                     /locus_tag="ACHOWS_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0048695.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARAARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEA
                     IAREWYEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            8350..9051
                     /locus_tag="ACHOWS_003161"
     CDS             8350..9051
                     /locus_tag="ACHOWS_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048696.1"
                     /translation="MPVVNDFPDLSHWFTVQEFTITQAALLLSGIDPLDYPTLEMVKE
                     EKHERWRMAYGFAYGMVTAIRRGVLTPVWIEYYPFWDVQVVSGSPRTEIPDRLKKISM
                     DDTIITRYSLQEWVKNEKVDYVRPSRSISDKEIKPSNQSVIIDAESRVINSEETVLLT
                     YGHKSEGLEYVEDAIKNFWATYEPENPQTAPTKTEIEEYLMKMGAGSNMAKAVNLILR
                     PFSLQQVGRRKKKNK"
     gene            9302..9499
                     /locus_tag="ACHOWS_003162"
     CDS             9302..9499
                     /locus_tag="ACHOWS_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015462155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0048697.1"
                     /translation="MSNNLIRLSDVMRRTGYGRAWIYKLIAQERFPKPVKVGSRSIAF
                     VESEIDEWINQRIAESRGTAA"
     gene            9555..10274
                     /locus_tag="ACHOWS_003163"
     CDS             9555..10274
                     /locus_tag="ACHOWS_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048698.1"
                     /translation="MKRSYMPVQGQGFVQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIKSHQSRLRELGSLPRQSVKELTYLKSGKPGRKRGRPEISYLLNELQLDY
                     LLRVIRGRDAERINQFKLDVTKAFAKHRAAEPVRREYLPGYHESRDGLKALGAERHHY
                     INLARAENRVTGLVAGERSTASEQQLGLLIVMQKIEQAAFDEAIQSGMTPTEAVREVA
                     RRMETFASLMTSAPSLGVNHA"
     gene            11473..11868
                     /locus_tag="ACHOWS_003164"
                     /pseudo
     CDS             11473..11868
                     /locus_tag="ACHOWS_003164"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            11899..12087
                     /locus_tag="ACHOWS_003165"
     CDS             11899..12087
                     /locus_tag="ACHOWS_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFQ0048699.1"
                     /translation="MATTPALVHSQTAFIWRFIIFGASESQIIHVTAWTEREARNRCP
                     SGCVAVFAARIRQEASHG"
     gene            12433..12618
                     /locus_tag="ACHOWS_003166"
     CDS             12433..12618
                     /locus_tag="ACHOWS_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048700.1"
                     /translation="MLKTFRVFSRAVNKRGHTIGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            12622..12816
                     /locus_tag="ACHOWS_003167"
     CDS             12622..12816
                     /locus_tag="ACHOWS_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048701.1"
                     /translation="MCNFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDMQ"
     gene            12883..12996
                     /locus_tag="ACHOWS_003168"
                     /pseudo
     CDS             12883..12996
                     /locus_tag="ACHOWS_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            13002..15143
                     /locus_tag="ACHOWS_003169"
     CDS             13002..15143
                     /locus_tag="ACHOWS_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFQ0048702.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGKLA
                     GDKTPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKTSEQSEDELKPRVECRPDG
                     VYWVTPKVDKQSGEIIRPETWLCASVELLGAGVIGNEHYRVMRWTDSTTNRLVTMAVP
                     CCGIGDSDGWRLLKANGLKVTTNGKHRIPLADWMQLGGQHEEWHLSTKAGWHFGAYIM
                     PDGTVIGESDKPVLFTGKSAAINGYSVAGTVDSWRDSVARLAGGNPFMMLGIATSLSA
                     PLVGLVGADGFGVHFFENSTAGKTTTQSVASSLWGDPEAQRLTWYGTALGIANEAEAH
                     NHGLLPLDEIGQAASARDVYVSAYTLFNGFGKLQGAKDGGNRELKSWRTVAISTGEVD
                     IETFLKTEGIKVKVGQLVRLLNVPIEKATQLHGYANGKDHADALKAAWKENHGAAGRE
                     WVKWLAAHQQEAKDTVRECRERWRNLIPESYGEQVHRVGERFAILEAALVLSGHVTGW
                     AVQECRDAILHNFNAWVKVFGTGNKEHKQIIEQAEAFLAAYGMSRFAPVNYDPASLPI
                     PELYGYRESDGRYDEPVLFYVLPDPFGSHVANGFNKDAVAKVLHEAGMLKRPSSGRGW
                     QIRTPRLKHLKGARLRVYGLLLAQDHDTESD"
     gene            15504..15833
                     /locus_tag="ACHOWS_003170"
     CDS             15504..15833
                     /locus_tag="ACHOWS_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048703.1"
                     /translation="MSEITVKNISPAVAGWWAKFRDDDGTEWYSPIAAWALCEVDYFG
                     AGNTRREILPVLTSELGMGPHPSDEGYCACLYLPDEKFTLSGEQGSFAWYPVNSNSNN
                     GNSGAES"
     gene            15830..16249
                     /locus_tag="ACHOWS_003171"
     CDS             15830..16249
                     /locus_tag="ACHOWS_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048704.1"
                     /translation="MTAKHTKNHNSVVKFVSARGRPTLLVPGKHLHYCDENHIPIVIV
                     WKRTVYADVTWLYDSLALMHRDLFEREEFRLNIEERAEKIYEQYAANSKRAARAITHH
                     FMTLYDLKAEDAEKAACDLFDMTMGIIQEYRNRERRP"
     gene            16246..16695
                     /locus_tag="ACHOWS_003172"
     CDS             16246..16695
                     /locus_tag="ACHOWS_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0048705.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALDRAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            16712..17179
                     /locus_tag="ACHOWS_003173"
     CDS             16712..17179
                     /locus_tag="ACHOWS_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFQ0048706.1"
                     /translation="MSQQTTQQGQTTRKPSRRIPWQHMTKEQRKNHRRVNRLCEYWPE
                     LFNLSEVRPLKNGVISDMLQEVRTRNIPVGKGALRGAVITYTHMTRYLKALIAGGPRY
                     DLNGKQCGSVTDAEKAAAEEVLAKRLAEAKQRSNQRQPCGGDDSDNRQRGTET"
     gene            17148..17441
                     /locus_tag="ACHOWS_003174"
     CDS             17148..17441
                     /locus_tag="ACHOWS_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFQ0048707.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWAEVLLHAGTDREREEAARRRA
                     ICISKTRRMPEQLVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALS"
     gene            complement(17892..18278)
                     /locus_tag="ACHOWS_003175"
     CDS             complement(17892..18278)
                     /locus_tag="ACHOWS_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048708.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVSVEIGNPPFDVSGVAYLLYGMD
                     SHNNRHQIESTRRTLEAMVRDGLLERVNVYERRDSVGHDVRRKVARYGLPGHSVVARD
                     SEGRNGAIDGECIRINATDVVLVEGQ"
     gene            18475..18906
                     /locus_tag="ACHOWS_003176"
     CDS             18475..18906
                     /locus_tag="ACHOWS_003176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048709.1"
                     /translation="MKKIDSLRISKVAIISVSLLFLSACSTNNIENMPRDARVKFALM
                     RAYSDNKFDNSTVIGEVLGLSCARQLGKTLVTPLPGGSAIATSSEGDITSESDAIQDM
                     RYKAAMMGGDAIVNVVCKSGGVDWIHNCWSTVKCVGDVIKK"
     gene            19403..19564
                     /locus_tag="ACHOWS_003177"
     CDS             19403..19564
                     /locus_tag="ACHOWS_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048710.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATQPDSV
                     SAENQADVT"
     gene            20232..20624
                     /locus_tag="ACHOWS_003178"
     CDS             20232..20624
                     /locus_tag="ACHOWS_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFQ0048711.1"
                     /translation="MRDIQQVLERWGAWAANNREDVTWSSVAAGFKGIIPAKITSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLIDYYVLGKTFMALAHKHECSDSLIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            20914..21120
                     /locus_tag="ACHOWS_003179"
     CDS             20914..21120
                     /locus_tag="ACHOWS_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048712.1"
                     /translation="MEGVVSHEQAGHRASKNHRFVLFFSQGKNATGINPSSGGISRQH
                     DEQAAQPDTASRRYYDFRNTFSKA"
BASE COUNT         5990 a         5095 c         5767 g         5289 t
ORIGIN      
        1 tcttcaatga tcgactttta agtaagtctg gaaggtaaag ctatggagct tgctactacg
       61 cagtcagtat tgatgcaaat tcaaccgaca attcagcgtt ttgccagaat gcttgccagc
      121 gttttgcagc ttgaggttga gatcgttgat gaaaacttgt gtcgcgtcgc cggaacgggc
      181 gcgtatggga agtttcttgg tcgccagttg agcggcaact cacgcctgct ccgccacgtc
      241 ctggaaacga aaactgaaaa agttgtgaca cagtctcgct tcgatcccct ttgtgaaggt
      301 tgcgatagta aagaaaattg ccgcgaaaaa gcatttctgg gtacgcctgt cattttacag
      361 gatcgttgtg ttggggtgat aagtttgatt gccgttaccc acgagcaaca agaacatatc
      421 agtgataatt tacgcgaatt ttctgattac gttcgccata tatccaccat ttttgtttcg
      481 aaacttctgg aggatcaggg gccaggagat aacatcagta aaatattcgc gaccatgatc
      541 gataatatgg atcagggcgt tttagttgtt gatgacgaaa atcgggttca gtttgttaat
      601 cagactgcct taaaaacact cggtgttgtg caaaataata ttattgggaa acctgttcgt
      661 ttcagaccat taacatttga gagtaatttt acccatggac atatgcagca tattgtttcg
      721 tgggacgata aaagtgaatt aatcattggt caattgcata acattcaggg ccgacaatta
      781 tttttaatgg cgtttcacca atcgcatacc agtttttctg tagcaaatgc acctgatgaa
      841 ccgcatattg aacaattggt tggcgagtgc cgtgttatgc ggcaattaaa acgactcatt
      901 agccgtattg cacccagccc atccagcgtt atggtggttg gcgaaagcgg cacgggtaaa
      961 gaagtcgtcg cccgtgcaat ccataagttg agcggaagac ggaataaacc ttttattgct
     1021 atcaactgtg ccgcgattcc ggagcagctt ctggagagcg aactgttcgg ttatgttaaa
     1081 ggcgcattta ctggcgcttc tgccaacggt aaaacagggt tgattcaggc ggcgaatacg
     1141 ggcacgctgt ttctcgatga aataggtgat atgccattaa tgttgcaggc taaattactg
     1201 cgcgctattg aggcacgtga aattctgccg attggtgcca gtagcccaat acaagtcgac
     1261 attcgcatca tttctgcaac taaccagaat ttggcccagt tcatttccga aggtaaattc
     1321 cgcgaagatc tcttctaccg acttaatgtt atcccgataa ctctgccacc gctgcgtgaa
     1381 cgtcaggaag atattgaact actggtgcat tactttttac atctgcatac ccgtcgtctg
     1441 ggatcggttt atccgggcat tgctcccgat gtcgtcgaaa tattgcgtaa gcatcgttgg
     1501 cccggaaacc tgcgcgagtt aagcaatttg atggaatatc tggttaacgt ggttccttca
     1561 ggtgaagtta tcgacagcac gctattgccg ccaaatctgc tgaataatgg cacaacggag
     1621 caaagtgatg taacagaggt cagtgaggcg cacctggcac tcgatgatgc gggcggcacg
     1681 gcgctggagg agatggaaaa gcaaatgatc cgcgaggcgc tttcacgtca taacagcaag
     1741 aaggaagttg ctgatgaact gggcatcggc attgctacgc tctatcgcaa gattaagaaa
     1801 tatgagttgt taaacacata acctcagtga atccggcctg aattcaggcc ggatatcctt
     1861 ttacttcgtt ttctcgcaat ccagaactgt atttacaatc atctgatacc ccgtgcagcg
     1921 acaaagattt cccgccagtc cacgacgaat tcccgtaatg gttaatggct tctcgcgtgg
     1981 tttcgccagc attgccgtgg tagccataat caggcccggc gtacaaaacc cgcactgcac
     2041 tgcgccggat ttcgcataag cctgctgaac atgagaaagt tttccgcctt tcgcttcacc
     2101 ttccagcgtg cggatctctt ttccttcagc ccaggcggca aggtataagc aactgtctat
     2161 tgctgtgccg tcgaccagca ccgtacaggc accacattca cccacgcagc acccttgttt
     2221 gacacttagc agtccttgtt cgcggagtaa ttccgagagc ggcgtgcctg gtgcggcgtg
     2281 aagctgaaaa ggtatcccgt taatggtgca ttcgatggta attgtttcgc tgtgattcat
     2341 tgcaattttc cccccgccgc ggcgacggct tcgctaatca cttttttggt catcgtctgg
     2401 atgagatgca gacgaaactc tttactggcc cgccatgacg aacgcggggc gacatcttgc
     2461 aggactgatt cgctgatggc ttccagcgtt tgcaggttta atggcgcatt ttgtgcagtc
     2521 tgttcggcat gttggcagcg aattggcgtt ggcgcggcaa caccaaatgc caggcgtaat
     2581 tcgctgaaat tgccgttatc cagttggcaa tgtgcggcgc agccaatcgt tgaaatatcc
     2641 attgcgtcgc gcatggcata tttaaaatgc gcgctgcccg cgtgttcttt cggctggggc
     2701 ggaaaatgaa aggcgacaag gatttcgtca tgctcaagag acactttgcc cggcccggtg
     2761 tgaaagccat taatcgggac gaaacgaaca ccgcgcgggg agtggatctc cagtttcgcg
     2821 tcataaatta gcgttggcgt ggcagaatct gcgctggtgg caccgttgca aatatttcca
     2881 ccgtaggtag cgacgttacg gatctgcggt ccggcaatgg atgaggccgc agcacataac
     2941 gccgggagat gacgttgagt tacaggatct tcaatgagct gggtaaatgt cgttgcagag
     3001 ccgatacgta gtgagccatc ttccgccagc gtaattcccc gcagctccgc cagattatga
     3061 atatcaacaa tatggcgata acggtcattg tgatggtgga gctgaatcag tacgtcagtg
     3121 ccaccggcga gcagtttggc ctgcgggttg tcagccagca ggttgatggc atcggcaagg
     3181 gttgctgcgc gatggtaaga agcaaaatca aacatgatgt tatcctcaaa tcaatcctgc
     3241 cagatggaac tcttcatata accgttttgg cgtcagcggc agtgtattga ttgcaacacc
     3301 ggtagccatc ttcaccgcgt tacgaatagc agcggcaaca ggaattattg gtggctcacc
     3361 cagtgactta tgtccgtatg cggattgcgg ctcattgatt tcgacgaacg cgctttccag
     3421 ttgtggcaga tccggcatgg tcggcatttt gtaatccagc agattggggt tacggaccac
     3481 gccgcttttc gcatcaatga tcatctcttc aaatagcgcc cagccaatgc ccattcccat
     3541 tccgccgtgt acctgacctt ctgccagtag cggattaaga atatggcccg aatcatgaac
     3601 gttgaggatg cggttgatgg tgactttaca cagcgcaata tcgaccgtca gatcaacaaa
     3661 ggtacagcca aacgctggtg ggttagtggt ggttttgatg gagctttcgg cagagagctg
     3721 cccgccgcgt tcagggtggt agaaggcgtc catcgccaaa tcttttaacg acattaatgg
     3781 ctcttccggt cgttcaacca gcacgatatg gccttttatc agggtaagat tcatcgctga
     3841 ctgatgtagc atgacagcgg catgagcgat gattttctcc tttaataaca gtgccgcact
     3901 gcgcagcgca ggcgcggcaa catagctctg gcgtgaggca aatgcaccgg gatcgaacgg
     3961 cgtaacgtcg gtatcttggg ttgaaataac gcgaacgtcg ctgaccggaa cccccacggt
     4021 ttctgctacc atttgcgaga agacggtgtc ggccccctga ccgatttctg tcgcgccgct
     4081 ttgcacgttg atggttccat cctgattcat caacaggcgc gcgcctgcta tttctacgcc
     4141 gacaggccag gtgttagagg tgtagctaaa acaggcgacg ccaacgccgc ggcgtaaatt
     4201 gccttgctgg ttctggcact ctgcacggcg tttttcccat tcaaagattt tccggccttt
     4261 ttcaagacac tccggcaacc ctgcgctgta aatacgtttg cccgtgagcg gattagcatc
     4321 tccttcgcgg gcggcgttgc gtaaacgaat ttcaacagga tcaataccta acgctgtcgc
     4381 ggcgtcatca agcatagact caacggcaaa tacgacttgt ggcgcgccat aaccacgcat
     4441 cgcaccagcc gaggggaggt tggtatagca ggtctttgaa ctgtaagcgt aggcacaacg
     4501 aggataaagg taagcgactt tatttccgcc cgcagaagcg atggagtgcc cgtgagatgc
     4561 ataagcgccg gtgttagaca gaacatccag actataacct ttcaatgttc cgtcgcggtt
     4621 cacgcccatt tgcccgtcaa tagtaaaagc gtggcgggta cgggttgcga ggaaacactc
     4681 ttcacggcta agggaaactt tcaccggaat gccgccaagc ttgctggtca ggaatgccgc
     4741 cattggctct tccagtacat cctgtttatt accaaaaccg ccaccgacaa acggtttgat
     4801 gactcgtacg catgaccagg gaatattcag cgcctgacca accacacggc gaacaatgtg
     4861 cgggatctgg gtgctggaaa cgatggtaat tcgcgagtca tcctccatcc atgccagcga
     4921 tgtcacgctt tccatatgac aatgttgaat aacgggagtc tgatagtgcc cctgcacctg
     4981 gtaatcggcg gcatcgattg tttgttggac attacccgtc gacatcgagc tttgttttag
     5041 taaattgccg ccgttatgga ttggtgcagc gtcatctgcc agcgccgctt ctggcgtggt
     5101 gataacgggt aattcttgcc actcaatgct gaccaattgc gccgcttttt ctgccgtgag
     5161 ttcatcacgg gccacgacga tggcaacggc gtcaccatga tgacgaacat ggcgagttag
     5221 cagtgcgcga tcggcggtat cgcgcttgtt ttcgtcaagt gtccaggcat gcccagctgt
     5281 agcgaatggg atatcaggca catcttccca ggtaaaaatc gccagtacgc ccggtaaact
     5341 tctggcttgt tcatcattaa tacttatggc ataaccatgt gcgatagggc tacgtacata
     5401 tttcgcgtaa cacatgcccg ccataacata atcgtcagta tatcgtgccc gcccggtgac
     5461 cttagcaatg gcatctacgc gcatgcatga ttcacctgta gcggttgctt cccgcgcttc
     5521 catagaaatc ccctcgattt gaacatttgg ttcttatggc aaagatttag tctaagcagg
     5581 caagatttac gccagaaacg acgataagcg ttaagggagg aaggaagtgt gatgatgtta
     5641 aaaaaacaca gatatgatac gaaacaggac cctgaatagg ggcagtgata cgtatcaata
     5701 tgagtggcga taaaatcaat gtgataaatt gggattatta cgcatcagga tgggaaatag
     5761 ggtaaatgag tcagaaaatt cgatacatct acagtgaata ttttgctgat gatacttccc
     5821 tttgccaaat gaaattaacc tctttctcta ttaccgcgtt tttccagaag caagagattg
     5881 ggtgtcaaga ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt
     5941 gagtgcggga cgcctgaata aaaaatctct gggtatcgtg atgttgttat cgattggact
     6001 gcttttggcg ggctgctcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt
     6061 ttacaccgtg aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaactagcgt
     6121 aaaagagctg gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa
     6181 actaaaactg ggtggggcga aaaatagtag tacacgtaaa tcaaccgcca aatcaacgac
     6241 caaaaccgca tcggttacac cgtcatcagc agtaccgaaa tcttcctggc cgccagtagg
     6301 gcaacgttgt tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagctga
     6361 tggcggcaat aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg
     6421 tgcaggaaag gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat
     6481 taaacacagt gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa
     6541 tgggcaaagc gtgaaggctg ggcaaaaaat cgccactatg gggagcactg atgcggcatc
     6601 ggttcgcctg catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt
     6661 gccgcctcag ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg
     6721 gcacttgcta aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt
     6781 tcaatggtag aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct
     6841 ccaaacaaac atctaccaac gtctactaaa gtctaccaat cccccgaata tcaaggcctc
     6901 cagcgttttt cctcgtctac aaccgtctac agtcatctac ctaaatctac acacagaagt
     6961 gtatagtaat gcgtatagga caaaatcgtt ttttgggggc tatacacatg ttgctttcag
     7021 accttgctat ccgccgcgcg aagccaaaag agaaagccta tacactcaat gacggcaacg
     7081 ggctttcgtt actcattgag ccgaacggtt cgaagggctg gcgattgcga tacaggtttg
     7141 caggcaagcc caaaatgttg tcgctggggg tctatcctga ggtgtccctt tctgatgcca
     7201 gggcggcaag ggatgaggcc aaaaggctta tcgctggtgg agttaatccg agtgaggtca
     7261 gaaaggccca aaagcgagaa caggcgaaca aatgcggtaa cacctttgag gcgatcgccc
     7321 gtgagtggta tgaaaaacgc ctggaccgat ggtctccctc atatgcagag gaaatgatag
     7381 agacgtttga aaaagacgtt tttccgtaca ttggaggcag gcccatcgca gagattaagc
     7441 cgatggaatt gctcgcggtt ctctcccgtc ttaatgaccg tggcgcaact gaaaaattgc
     7501 gtaaggttcg ccagcgatgt ggcgaagtgt tccggtacgc aataattacc ggacgcgctg
     7561 aatataaccc cgctcctgat ctggcaagcg catttgcacc acacaaaaaa gagcattacg
     7621 cgtatctgaa agttgatgag ctaccggagt ttttccgcgt ttttaatacg tacaccggaa
     7681 gccagatcgt caagctgggt atgcgattgt tgattcttac aggacttcgc ccaggtgaat
     7741 tacggcaggc agagtggcag gagattgatt ttgataatcg tctgtgggaa gtgccgaaag
     7801 agcgaatgaa aatgcgccgt cctcattgcg tgccactttc agaacaggcg atcgctattc
     7861 tggaacagtt aaaacacatg acggggcagt atccgtttat tttccctggt cgaatacagc
     7921 acagtaagcc gatgagtgaa atggctatga acgtgcttat tcgtcgtatt ggctatgcag
     7981 ggcgcgttac tggtcatggg ttccggcaca ccatgagtac gattttgcac gagcaggggt
     8041 ataactccgc atggatagaa acgcagctgg cgcacgtcga taaaaactcg atccgtggca
     8101 catacaatca cgcgcaatat ctggacggac ggcgggagat gctccagtgg tacgccgact
     8161 atatggactc actggaacag ggcggaaacg tggtacatgg caagtttgga aaatgtggat
     8221 gactggttga gtatacagta gtagactttg agcgactaaa gaacaggctg tgtctaggat
     8281 cgctcccgaa accccgtaca cctctgcgga ctgacacagc caccactaca gagagtgcga
     8341 ggtgtgcaca tgccagttgt gaatgatttc cctgacttaa gtcattggtt tacagtgcag
     8401 gagtttacta ttactcaagc tgctttattg ctgtcaggta ttgatcctct cgactaccca
     8461 actctggaga tggtcaaaga ggaaaaacac gaacgatgga gaatggcata tgggtttgct
     8521 tatggaatgg ttactgcaat tcgtcgtggt gtattaactc ctgtgtggat tgaatattat
     8581 cctttttggg acgtacaagt agtatccggc tctccccgta cagagatacc agatcgtttg
     8641 aagaaaatat ccatggacga cacaattatt acccgttatt cattacagga atgggtaaaa
     8701 aatgagaaag ttgattatgt aagaccctct cgctctatat cggataagga gattaagcca
     8761 tctaaccaat cagtaataat tgatgctgaa tcgcgcgtga ttaattctga agaaacagta
     8821 ttgctgactt atggtcataa gagcgaaggt cttgaatatg tagaagacgc aataaaaaat
     8881 ttttgggcaa catatgaacc agagaatcca cagacagcgc ctacaaaaac agagatcgag
     8941 gaatacctaa tgaaaatggg ggctggaagc aacatggcaa aagcggtaaa tctgatacta
     9001 cgcccattca gcttgcagca agttggaaga agaaagaaga aaaacaaata gttaaatagt
     9061 gctcaccctt cgtgaaaagg gtggtactat ttgctaaaaa tagttttttt atagtgagga
     9121 atcatagtta ggcgtgcatt ttctttgctt attgatactg gaatagtgac accattatta
     9181 aaataatagt gggtgctatt tcaatggaat agtgcccacc attaaaaatg gtatccacct
     9241 ataaatatat tgtcctttta tacatcattg tagacggtta tagacgttac gaggattgaa
     9301 tatgtcaaat aacttaattc gtctttctga tgtaatgcgt cgtactggtt acggtcgcgc
     9361 atggatctat aaactcattg ctcaagagcg tttccctaaa ccagtgaaag taggttcccg
     9421 ctcaattgct ttcgttgaga gtgaaattga cgagtggatt aatcagcgta ttgcagaatc
     9481 acgcggaacg gctgcctgat taaatagcta aggggctatt gccccggcaa ttaatttgat
     9541 agcaggagtt atcaatgaag cgttcatata tgcccgtaca ggggcagggc ttcgttcagc
     9601 ctgaaatcag ccagaacgat atattgattc gccacaaagc ggagtcgcgt attgattccc
     9661 gcgttatttc tgagcgagca ggaatacaac acgaaagtat cattgcgacg attaaatctc
     9721 atcaatccag attgcgcgag cttggctcac tgcctcgcca gtcagtcaag gaattaacct
     9781 atttgaaatc gggaaaacct ggccgcaagc gcggtcgtcc tgaaattagt tatttgctga
     9841 atgagctaca gcttgactat ctgttacgcg ttatccgtgg gcgtgacgca gaacgtatca
     9901 accagttcaa gctggatgtt acaaaggcat tcgcgaagca tcgcgccgct gaaccagtcc
     9961 gccgcgaata tctgccaggc tatcacgaaa gccgtgacgg actcaaagca ctgggcgcag
    10021 aacgtcacca ttacattaat ctggcacgcg cagagaaccg tgttacaggt ctggtggctg
    10081 gagaacgcag cacagccagc gagcaacagt taggcttact gatagtcatg cagaaaatcg
    10141 agcaggcagc atttgacgag gcgatacaga gcggcatgac accgaccgaa gcggtgcggg
    10201 aggttgctcg ccgcatggag acgttcgcca gcctgatgac gtcagcgccg tctctggggg
    10261 tgaatcatgc gtaattatgt tatcccgccc aatcatgaag gcggtttata cacggggtta
    10321 cagccatcag gggatgagag gggcagcaaa tacttcaaat tataataatt agcttgctaa
    10381 ctgtttttgt gcgcaatttg ccattcaacc cccggtgctg gcttcggtgt tgtcattcag
    10441 tttgtgcatc atgtttgaac tgacaaggca ctaccagagc ctaagccagg gagggagtgg
    10501 cctttctggt agtgggccgc tgttggcgtt ggcacagtgt taaggtatgt agggtatgca
    10561 ggtaagggat gccccctcaa atcccccata agtaacatga aaaaataacg cgagtcaaat
    10621 taataaaact tatggtgttg aatcaaaagg gaatttattt tcaaaacata atttcacaaa
    10681 tgaaatgttg ctcataattt aaccgcgcaa agtactacca caaccccaat atataagggt
    10741 atcagcagaa tttaataacg aaaatcagga gtaaataaga tgattatcat gcctgtaaac
    10801 atggctggta accgttacag gccaccagca cgctaccacc gtcagaacat gatacggcat
    10861 tgcatggcgg tccgtggctc tgatttctgg caaggcgcaa aatcccgcca gcagcttact
    10921 catgattaca tgcgccatat cgtgagccat ttcgccgctg agcgcatgaa acacgttaag
    10981 caatacttgg gggaggtttt atcaaattat cgcgctggtg gccctgtacg gcgcgaatat
    11041 ccgcatgatg caggaatggc aggcaaaaca aaggcgacca cgaaagggtc gccagtggag
    11101 ataaaggaaa tcaagcgcat caccaactat gccatgagtg tagctggtgg acaaggctat
    11161 cagccaggcg gtcggttttt agtggctttt aaccgccgtg ggtatttctc gacaaaagcc
    11221 tcaagggcaa atcgtgttgg cctggtgagt ggctgtggat taagtaacaa cctcctttgc
    11281 cgttgtgctt tttcgcgctg tcgctcaagt atggataatg cgatttcctg tagttcttca
    11341 tttgtcattg tctgtacccg taaagcggtg cgctgtagta cctcatatca cagcgcaggt
    11401 attttttatg gttgtaattt gcgtgatgat tcaggtcgct ttgcgacata ctacataaac
    11461 ataaataacg tgatgttatt atgtgctttc aaagaaaacg caggtatttt tcgcacaaac
    11521 ttctctcgca acacatccga ccacttaagc aaaaaagcgg catatactga ggctatccct
    11581 tataaaacgg ggatcgggcg taggaacccg tttaactgta aggcgataaa tgacgccaga
    11641 agcgtctttt tttctgccag cgctttacgc catatcaatg gcgttgtgta ttgtgcgtac
    11701 tcgtgcacca tccagtcatt tcacgagttg cattttgtac agcgccacca gcaaacaatg
    11761 gtgatgctgg cggggctacc gaaaggtagg ccggagtcct tacagtccgg tattcctacc
    11821 cccgccagtg tcaccgccca gtgtgagcgt aggaactcct gcggtgacgt tattcatcac
    11881 tgtaaggagg ctgccaacat ggctactacc cctgccctcg tacattctca aactgccttt
    11941 atctggcgct ttatcatctt tggcgcgtca gaatcccaaa tcatccacgt aaccgcctgg
    12001 acggaacgcg aagcgcgtaa ccgttgcccg tcaggttgcg ttgctgtatt cgccgcccgt
    12061 atccgtcagg aggcatcaca tggataaaaa catgttgatc gcctcttctt tcgggctgta
    12121 tgatgccgaa gtcgcccaca tggcgaccgg gtttagcagc ctgaatcaga tagcggacag
    12181 ccgcttacat tccgatatgc ggtttttttg tgtccgtaag cctacccata cccgcattat
    12241 ggcggggcgt aacgggggag cctttgcgct cgctggttcc tatctggcca gtctgctaac
    12301 cccgttacgt ctcgccacca tgtttagcag cgtagtagcg agactcctta atcaacagat
    12361 aggagccttt cacatggctg tatccgcacg cacatctgat ttttgtcgcc cgtatccgtc
    12421 aggaggtgac tcatgcttaa aaccttccgc gtcttttccc gtgctgttaa caagcgcggc
    12481 cataccatcg gcatcacaca gaacgtgaag gccgaaaacg tccaggctgc aatcagcaca
    12541 gtaattaaca aatcagctaa agccggttta tccggcgtaa ttatccacgc agttacatgt
    12601 attcaggatg aggagtaatt catgtgtaat ttttccgata tgactgttga tgaagcagca
    12661 aaaagcgcaa tgcaggcaga acttatttgt tctctgatga tgggtaacac aggtgaaatg
    12721 actgagggtg agattgagtc attgctcgcc ctgattaagc agcttacagg taaggctggt
    12781 agctggctga ttgcggcatc gggtgatatg caataaggga agaagtaaca ggatgaaata
    12841 aataccagcg aattaaagcg ttacatatca ggaaataaaa cgatgaaacg taaatattct
    12901 ggcttaactg ccagtagcaa aactcacccc gaatttatgt gcggggatat ttaccgcaat
    12961 gaatacggta gccacatgac gatgatcagc agaggtaaga aatgaaaagc gcaccgaact
    13021 taaaaaaaca gcctcgaggg aaaaaacacg ccgatacgga agtgattatt ttcgctggca
    13081 gtgatgcgtg ggcgcacgcg aaacagtggc aggagcagga cggaaaactt gccggagata
    13141 aaacaccgcc tgtcgtgctg gctgatgagc aacttaaaga tattggcaat ctgcaaattg
    13201 tacccgacgg gcgcaaatca gcgcgtatat acaaggccgg acacctcgat caggtgatgg
    13261 tcaaaggcat aggtcagaag ctggcggcgg caggcgtaca ggacgcagac tattaccccg
    13321 aaggtatgca ccataacgag cggcagaact ggcgtaacta tctggagact gagcgcaaaa
    13381 acatttctga cggactggtt attgagctgc ctgtaaagaa aaaaacatcc gaacaatcag
    13441 aggatgaatt aaaaccccgc gttgaatgcc gtcctgatgg cgtttactgg gttacgccaa
    13501 aggttgataa gcaatcaggc gaaattatac ggcctgaaac gtggttatgt gcttcggttg
    13561 aattactggg cgctggtgtc attggtaacg agcattaccg cgtgatgcgc tggacagatt
    13621 cgacaacaaa tcggcttgtc actatggccg tgccttgctg cggaataggc gacagtgacg
    13681 gctggcggtt actgaaagcc aacgggctta aagtcacgac aaacggaaaa cacaggatcc
    13741 ccctggctga ctggatgcag ttaggcggac agcatgagga atggcactta agcacgaaag
    13801 ctggctggca ctttggcgcg tacatcatgc cggacggtac ggttatcggc gaatctgaca
    13861 agcccgtttt gtttaccgga aaaagtgctg ccattaacgg ttattccgtg gcgggtacgg
    13921 tggatagctg gcgcgacagc gtggcgcggc tggctggtgg taatccgttc atgatgctgg
    13981 gcatagcgac gtcattatcc gcaccattag tcgggctggt cggtgctgat ggctttggtg
    14041 tgcatttttt cgaaaactcc acagcaggta agaccacaac acaaagcgtg gcatcgagtt
    14101 tatggggtga tcctgaagcg cagcgattaa cctggtacgg aacagcgtta ggcattgcta
    14161 acgaggctga agcacacaac cacgggcttt taccactgga tgagatagga caggcagcaa
    14221 gcgcccgtga tgtgtacgta tcggcttata cgctgtttaa cggcttcggg aaattacagg
    14281 gggcaaaaga cggcggaaac agggagctga aaagctggcg cacagtcgct atcagcaccg
    14341 gagaagtgga cattgaaaca ttcctcaaaa ccgaggggat aaaggtaaaa gtggggcagc
    14401 ttgtccgcct gcttaacgtg ccaatagaga aggccacgca gttacacgga tacgcgaacg
    14461 gaaaggatca cgcagatgca ttgaaagcag catggaaaga aaaccacggg gcagcgggtc
    14521 gtgagtgggt taaatggctg gcagcccacc agcaggaggc aaaggatacg gtaagggaat
    14581 gccgcgaacg gtggcgcaac ctgataccgg agagctacgg cgagcaggtc caccgcgtgg
    14641 gtgaacgttt cgccatactg gaggccgcac ttgtgctttc cggtcatgta acaggctggg
    14701 ccgtgcagga atgccgcgac gcgatactgc acaactttaa tgcctgggtg aaggtgttcg
    14761 gtacggggaa caaggagcac aagcagatta ttgaacaggc tgaggcgttt cttgcggcct
    14821 atgggatgag caggtttgcc cctgtcaatt atgaccccgc cagtctgcca attcctgaac
    14881 tgtacggcta cagggaaagc gacgggcgtt atgacgaacc tgttttgttc tacgtgctgc
    14941 cggatccgtt cggctcacat gtggcgaatg gatttaacaa ggatgccgtg gcaaaagttc
    15001 ttcatgaagc cggaatgctg aaaagaccat ccagcggtcg tgggtggcaa atcaggacac
    15061 cacggctaaa acacctgaaa ggggcgcgcc tgagagtgta cggccttttg ctggcgcagg
    15121 accacgacac ggaaagcgat taatctcgcg tataggcgat gaaaaaatgt tgtcccagtt
    15181 gtcccactac gtattaattg attgatttta ctaaaaatac aataaaaaag tgggacaaca
    15241 ttacagcatt ttgggacgac atcggggtat tttgggacga catagcgaca ttttgggacg
    15301 acacaacgag cgttaattac cctggctggt gactgggaca acataaaacc cctcatgttg
    15361 tcccaaaatc ggctaatgtt gtcccagttt tcggagtgta tttttaatta aatcaatgga
    15421 ttatgtgtaa gtgggacaac tgggacaact gggacgacgt gtttttgtat atatataaaa
    15481 aaataaattg agaggatgaa ttcatgagtg aaattactgt taaaaatatc tcacctgcgg
    15541 tagctggctg gtgggcgaag ttcagagacg acgacggcac ggaatggtac agcccgattg
    15601 ctgcatgggc gctttgtgag gttgattatt tcggtgcggg gaacacccgc cgcgaaatct
    15661 tacccgtgct gaccagtgag cttggtatgg gtccgcaccc atccgatgaa ggctattgcg
    15721 cgtgtctgta tctgcctgat gaaaagttca cgctctcagg tgagcagggg tcttttgcct
    15781 ggtatccggt gaacagcaac agtaataacg gcaattcggg ggctgaatca tgacggcgaa
    15841 gcacacaaaa aaccataatt ccgtggtgaa gttcgtatct gcccgtggca ggccaacgct
    15901 tttagtgcca ggaaaacatc tgcattactg cgatgaaaat catatcccga tcgtgattgt
    15961 ctggaagcga acggtatacg ccgatgtgac atggctgtat gactcgctgg cgttgatgca
    16021 ccgtgacctg tttgagcgtg aagagttccg gctgaatatt gaggaacggg cagaaaaaat
    16081 ctatgagcaa tatgcagcaa acagcaaaag agccgcacgg gcaatcactc accatttcat
    16141 gacgctgtat gacctgaaag cagaagatgc agaaaaagcg gcatgtgatt tattcgatat
    16201 gacaatgggt attatccagg agtaccggaa cagggagcgc agaccatgac agcacagatt
    16261 gcagcttacg ggcggctggt ggacgacccg caggaaaaac agaccagcaa gggcacaccc
    16321 atgacgctgg cgcgtatggc ggtatctttg ccatgcagtc aggcacagga cggacaggcg
    16381 acgttatggc tatcagtcct ggcatttggt aagcaggccg acgcgctggc gagacatgaa
    16441 aaaggtgacc ttatcagcgt ggcgggtaac atgcagatca gccagtggac cggacagaac
    16501 ggagaaatac ggcagggctg gcaggttatc gctgacagcg taatcagtgc ccgttcggcg
    16561 cgtcctggcg ggaagcgggg gcagcagggg aaggcgaccg aagcactgga cagagcaaaa
    16621 cgacagaccg gacagcaggg aaatcagcca ccagcgggag gtgatgaccc gttctctgac
    16681 gatattccgt tctgaatgac aggagcacaa catgagccag cagaccacac aacagggcca
    16741 gaccacacgt aaaccatcac ggcgcatacc gtggcagcac atgacaaaag agcaacgaaa
    16801 aaaccacaga cgcgtaaacc gtctgtgcga gtactggcct gaactgttta acctctcgga
    16861 agtcaggccc ctcaagaacg gcgtaatcag cgatatgttg caggaggtca ggacaagaaa
    16921 catacctgta ggcaaaggtg cattacgtgg ggcggtaatc acttacaccc acatgacgcg
    16981 ataccttaag gcgctgattg ctggtggacc acgttacgac ctgaacggga agcagtgcgg
    17041 aagcgtgacg gatgcagaga aagcggcagc ggaggaagtg ctggctaagc gtctggcaga
    17101 ggcaaagcag cggagtaacc agcggcaacc atgcggaggt gatgacagtg ataacagaca
    17161 gcgtggcaca gaaacttgag gagcgcggtt tatggcgacg tgcggcgacc cgatgggctg
    17221 aggttcttct tcatgcagga acggacaggg aacgcgagga agctgccaga cgtcgggcga
    17281 tatgcatcag taaaacccgt cgtatgcctg aacaactggt cacctttggc gatgtgaaaa
    17341 aggctgctga ccggacgctg aaagagatgg ggattaatcc acaggatgaa tggcgaaatt
    17401 acagcttcag tgatgcaggc gacgatttag cgctatcgtg atacgcatct catcgccgta
    17461 tttgctcaat tatcgcgatt cgctataatc accaccgaaa ggccgttgat ggtgttctcc
    17521 gcaggcaacg ggagcctcgc cgaatgaagc cagccagcgg cttttcggca ctcattttga
    17581 tgtgtacttg tcacacacat cgggatgagt gaacgacgaa aacatcacgg tgatttcgtc
    17641 atgccgcgat aagaatgcgc ctctttgggc aactggagcc ctctgtacag cgggttattg
    17701 cggcaaagtt gtgataaaaa cgcgcctctt tgagcaattg aagctcactg taaagcggct
    17761 tatcacagca ggctgtagcg ggtgctcatc tccgggcaac tggagccctc tgtacagagg
    17821 cccgttacgg ctgaccctga atgatttcgc cccgtgcaat tgaagcaccc agcggagtta
    17881 ttcagggttt tttattgccc ctccaccagc accacatctg tagcattgat gcgtatacat
    17941 tcgccgtcaa ttgcaccatt ccggccctca ctgtcacgcg caacaacaga atgaccaggc
    18001 aggccataac gcgccacctt tcgacgaaca tcatgaccaa cgctgtcacg ccgctcatac
    18061 acattaaccc gctcaagaag accatcacgc accattgctt ccagtgtgcg ccttgttgat
    18121 tcgatctgat gccggttgtt gtgtgaatcc atgccataca gcaggtaagc cacaccggag
    18181 acatcaaacg gcgggttgcc aatttcaacg ctgacccacg ccagattgtc aggagtgaag
    18241 taactgagga tctctttttt gtattgcgtc agtctcatag tgacctttct tgttgtagga
    18301 tgaatccctc atacaataag ccataaggca aattttgaaa ggacagaatc ggcggggtac
    18361 agatgatacg tgatgacaag gctgaagcgc tggaagcaca agggttatgc gatgggttgc
    18421 ggcaatatga ggcaaatatg aatattcaaa ttgttgtgtt gtgaggtacg caagatgaaa
    18481 aaaattgatt ctttacgaat aagtaaagta gctattatat cagtatcttt attatttctt
    18541 agcgcgtgtt ctacaaataa tattgagaat atgccgagag atgcgcgtgt aaaatttgct
    18601 ctaatgaggg cttattcaga taacaagttt gataattcaa cagttatcgg cgaagtgtta
    18661 ggattgagtt gcgccagaca acttggaaaa acattagtaa caccattgcc agggggaagc
    18721 gctattgcta catcctctga gggcgacatt acttctgagt cggatgcaat acaggacatg
    18781 cgatataaag ccgcaatgat gggaggtgat gccatagtga acgttgtatg taaatcaggt
    18841 ggtgttgact ggattcataa ctgttggtca actgttaagt gcgttggtga tgtaataaaa
    18901 aaataacagt tgccatactg aaggtattga ttaatacatg gaaactgcaa ttacgacgtg
    18961 tgattgcagc ttaccgttag ctacccctgt tacaattgcc ctcttccatc acagcaaaat
    19021 gctaccccct tcacagcccc ccctcggaag cccctccgat ggggtgaaaa atccctgaaa
    19081 aaatgcgcag aaaattgcgc aaaaaactgc gcaaatcgat cataaaaccg atcacaaaat
    19141 cgatcataaa accgatcaca cagaccagca gagaaaagcc aggtatgacg cggcttgcag
    19201 gggggaaagg ccaccggaaa aagtgcgcat ttttctcaaa aacgaaacgc aacaggaagc
    19261 gcaaaacgaa accgatacgc gatgatcgaa aaagcgcgca aatttaccgg attcgggtaa
    19321 gcaattttgc ctgatttggg caaaatacgg tgtcgccgta acagtgacat tgacaccaaa
    19381 acagtgacat acaggagggc cgatgaccga acgttcacca acagagataa ttcacgggat
    19441 tattgaagag caggaggaac gcgagttaat cctcatgatt gaagagcaga gagaacgcca
    19501 gcgcaaacag gaggccacgc agccggacag cgtgagcgct gaaaatcagg ctgatgtgac
    19561 gtgatggtgt cgaactgagt agctttaagc gactctgtga ctggttttaa acgagtcgca
    19621 aaaagagccg gagaaaaacc acgaatctgg ggttttttct aagcacgctg tacacggtgg
    19681 aacatatggt ggtcaggaga cggcaggagc aacgccagaa cgcgcccccg taccagtggc
    19741 ataagccgga gcggttacgg cggtaacttg ttgatattgt tccagagtgc aaatttgcac
    19801 tttggattca aaataccgtg gaggtaagta tctgatattg ttccaaagct catttttgag
    19861 ctctggtctg ccccgtttat tcaacttccg tcagtggtgg gagttgagaa acactgctgc
    19921 gtggtattac cctaacccat tgatgttttt ttgctcattt gctcgattat ttgagatgtg
    19981 tgtaaaactc aatttgagtt gttcaaattc accagtcgta aaaccatgat ttgtacatcc
    20041 ccgatatcgg ggtatgtaaa ccatccagac cggaccagtc agccctgatt tttacatccc
    20101 tcacttctga gggctgataa caaaccatct ttaagacggt tagtgctacg aaattttcgt
    20161 agctttaatt ttcatccagt gcatccccga attacgggta tgtaaccaca gctattacga
    20221 ggtgattgtt gatgcgtgat attcagcagg ttctggaacg ctggggagcg tgggcggcaa
    20281 ataaccgtga ggatgtaacc tggtcatccg ttgccgcagg atttaagggg attattccgg
    20341 caaagataac atcacgccca cagtgctgtg acgatgatgc gatggttata tgcgggtgca
    20401 tagcccgtct ttaccggaac aaccgcgatc tgcatgactt gctgattgat tactacgtgc
    20461 tggggaaaac gtttatggcg ctggcacata aacatgaatg ctctgattca ctcatcggaa
    20521 agcggctaca gaaagcggaa ggtgttgttg aaggcatgtt gatgatgctg gatgtgaagc
    20581 tggagatgga tcggtatgtt gagcgcgatt cagtgacggc ataacgcgca taaaaaaacc
    20641 gcagtcatga ggaaactgcg gcggagaagt cagacttgcc aatcaatcat gaaaaacgta
    20701 atcgcctgat aattatgcca cgagacaaag gacggtacac cctgaatggt cataatgacc
    20761 actcagacca tcaaggcaac tataacgaac atcgttacag ttggagcttt taccccgttg
    20821 ggggaaaagg tgcatacctg aatcatcatt atggtgatca agggtatgta aagcccacca
    20881 gcctgattaa aggttaacca gaaaaagcca ggtatggagg gagtggttag ccacgaacaa
    20941 gccggacacc gcgccagcaa aaaccacaga ttcgtgcttt tcttcagcca gggtaagaac
    21001 gccacgggga ttaatccgtc gtctggtgga atcagtcggc agcatgacga acaggccgcg
    21061 cagccggaca ccgccagcag aaggtattat gattttcgta atacctttag caaagcgtga
    21121 atcatcatta cggtagggat ggtcataatg accacacctc cccccaccgg aacagggagg
    21181 atgacctacg aaaatttcgt aagtcctacc ccgcacaagg attaaataat cactacaagc
    21241 gattgattaa acttaattat cgcgaatcgc gagaattagc cccgcagaaa caaggaaaga
    21301 agaattttac gaaaatttcg taaaatcccc ccacatctcc cacggttgcc cgttaagtgc
    21361 aagcgcacag gcacgacctc attaaccagt taccagcgct cacgctcaaa tatccacagg
    21421 aacggggcta aatcgcattc tggcgcaatg ttcactgtca gggcgagtat tcaccagaaa
    21481 acgtcataca ggcttacagt cggcttaaaa ttcgataggc ggataacagg gcgtggattg
    21541 ggaggcttac gaaaatttcg taacccccta cacaaaaagt gcaggttact acccgaaaag
    21601 tgtagtgcca ttgctggggc aaaaaaaaaa cgcagtgtga taacactacg gcacgccacc
    21661 aaagaaacat aacaaggaat caacaatgag taacacctgt aagccttcgg ggtgattgta
    21721 acacatgagc aatcttttac tggaaactgt ttttcactgg atcacgtatc gttatgggga
    21781 ttcccatatc ggagagcatc caccagcttc ataacggata gccggaaaaa ttccggtaaa
    21841 tatttgcctg agtaagtcat caagcgcctg ctaaatgtgt atagaaatgt gtataggatt
    21901 gattttagaa catgtgggaa tggctttatt gctggtggca tggcttgttg ttctgttccc
    21961 ttcgccgctc caatttatct tcacatcatc tacctgccat ttttgttgta tctgattttc
    22021 tatattatct taattcaagc gcttcagact tttcgcaatc gccgtaatac ttccccattt
    22081 ttttgtaaat aaaaagcgtt tacgcaatgc aatcgtcatg tatcttaaat agtcttattt
    22141 a
//
LOCUS       DAZKJR010000083        21962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKJR010000083 DAZKJR010000000
VERSION     DAZKJR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.083"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..540
                     /gene="uspC"
                     /locus_tag="ACHOWS_003180"
     CDS             112..540
                     /gene="uspC"
                     /locus_tag="ACHOWS_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFQ0048713.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(547..1971)
                     /gene="otsA"
                     /locus_tag="ACHOWS_003181"
     CDS             complement(547..1971)
                     /gene="otsA"
                     /locus_tag="ACHOWS_003181"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFQ0048714.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1946..2746)
                     /gene="otsB"
                     /locus_tag="ACHOWS_003182"
     CDS             complement(1946..2746)
                     /gene="otsB"
                     /locus_tag="ACHOWS_003182"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFQ0048715.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2913..3899)
                     /gene="araH"
                     /locus_tag="ACHOWS_003183"
     CDS             complement(2913..3899)
                     /gene="araH"
                     /locus_tag="ACHOWS_003183"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFQ0048716.1"
                     /translation="MSSVSTSGSGAPKSSFSLGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3914..5428)
                     /gene="araG"
                     /locus_tag="ACHOWS_003184"
     CDS             complement(3914..5428)
                     /gene="araG"
                     /locus_tag="ACHOWS_003184"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFQ0048717.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRTHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     ADQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5498..6487)
                     /gene="araF"
                     /locus_tag="ACHOWS_003185"
     CDS             complement(5498..6487)
                     /gene="araF"
                     /locus_tag="ACHOWS_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFQ0048718.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7284..7787
                     /gene="ftnB"
                     /locus_tag="ACHOWS_003186"
     CDS             7284..7787
                     /gene="ftnB"
                     /locus_tag="ACHOWS_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFQ0048719.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7865..8116)
                     /gene="yecJ"
                     /locus_tag="ACHOWS_003187"
     CDS             complement(7865..8116)
                     /gene="yecJ"
                     /locus_tag="ACHOWS_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFQ0048720.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8231..8317)
                     /gene="azuC"
                     /locus_tag="ACHOWS_003188"
     CDS             complement(8231..8317)
                     /gene="azuC"
                     /locus_tag="ACHOWS_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFQ0048721.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8580..8903
                     /gene="yecR"
                     /locus_tag="ACHOWS_003189"
     CDS             8580..8903
                     /gene="yecR"
                     /locus_tag="ACHOWS_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFQ0048722.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9075..9572
                     /gene="ftnA"
                     /locus_tag="ACHOWS_003190"
     CDS             9075..9572
                     /gene="ftnA"
                     /locus_tag="ACHOWS_003190"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFQ0048723.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9610..9849)
                     /gene="yecH"
                     /locus_tag="ACHOWS_003191"
     CDS             complement(9610..9849)
                     /gene="yecH"
                     /locus_tag="ACHOWS_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFQ0048724.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10039..11250
                     /gene="tyrP"
                     /locus_tag="ACHOWS_003192"
     CDS             10039..11250
                     /gene="tyrP"
                     /locus_tag="ACHOWS_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFQ0048725.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11301..11966)
                     /gene="yecA"
                     /locus_tag="ACHOWS_003193"
     CDS             complement(11301..11966)
                     /gene="yecA"
                     /locus_tag="ACHOWS_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFQ0048726.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIESAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12323..13324)
                     /locus_tag="ACHOWS_003194"
     CDS             complement(12323..13324)
                     /locus_tag="ACHOWS_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0048727.1"
                     /translation="MSVRKLSTGKWLCECYPYGAAGKRIRKQFATKGEALSYERRLMN
                     SNLGGEFQDGSGPRLSELVARWFEMYGKTLSSGEERKAKLEAICSRLGDPFASQFDKN
                     MFAIYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYADEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQILP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDTAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(13359..13616)
                     /locus_tag="ACHOWS_003195"
     CDS             complement(13359..13616)
                     /locus_tag="ACHOWS_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048728.1"
                     /translation="MVTNPQIPLTGLYVSKVNPSNRIVVTDVHIVLDDDDEPGDLPFY
                     LVTFVNEGDEDDMSAPSWELDPDEWEQLVDEKLFMRVEQSS"
     gene            complement(13815..14459)
                     /locus_tag="ACHOWS_003196"
     CDS             complement(13815..14459)
                     /locus_tag="ACHOWS_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048729.1"
                     /translation="MAVNSNGSDNRIAGRDYQEKNIQIERYDGSHTVNIAIPSSNDSD
                     PRPLVKGQRKALNSLVATIVDASNTEAFIIWQKLHAEIGVDSIDDITMNQYKTAESFL
                     CAMLERCKDHDARKALVSILLRNSEDGVLRQKLIRYCHINFGTGRLNDLTRSQLQMAL
                     SWLDQQSVEGEKKLTANNSADSQLQMNFQQIIKKYPKETGMCFVIGVLLGIILF"
     gene            complement(14475..14879)
                     /locus_tag="ACHOWS_003197"
     CDS             complement(14475..14879)
                     /locus_tag="ACHOWS_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0048730.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLSALSRFGLDVQYVVLGIHSPETYNDDEQELISRFRSASLDVKNAVIGALKGAV
                     SEKDSLSSEGRLNISGGNNRIAGRDYNETKGK"
     gene            complement(14969..15106)
                     /locus_tag="ACHOWS_003198"
     CDS             complement(14969..15106)
                     /locus_tag="ACHOWS_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048731.1"
                     /translation="MRAMLEDERCSLAAISSMMARSSADNRSASRCPVAVLRGIRDLV
                     S"
     gene            15178..15381
                     /locus_tag="ACHOWS_003199"
     CDS             15178..15381
                     /locus_tag="ACHOWS_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFQ0048732.1"
                     /translation="MQGITININVNAPYVSLKKYAEITGIPLNTCKKMLADGRIIIRP
                     KRARMEKPEVNLIAMLKDAIANS"
     gene            15402..15752
                     /locus_tag="ACHOWS_003200"
     CDS             15402..15752
                     /locus_tag="ACHOWS_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFQ0048733.1"
                     /translation="MKKNAIPPRPKHRTGTERHANRFSTSASRSNSHYSLQETHATPD
                     GHLVKQTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTAD
                     RLISGRSFQHPWGN"
     gene            15756..16010
                     /locus_tag="ACHOWS_003201"
     CDS             15756..16010
                     /locus_tag="ACHOWS_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048734.1"
                     /translation="MATTTKDMAEIASAYTIIIHRLIANNARNALNTAKPLWETKKDI
                     ISALQGLQKCADLAGDYAVYMAIEDTIEKIESGKSLSDFV"
     gene            16031..16273
                     /locus_tag="ACHOWS_003202"
     CDS             16031..16273
                     /locus_tag="ACHOWS_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFQ0048735.1"
                     /translation="MDKEYKVLINKALERFNFRLSMSGVAAEHAARDSLTRAIRSLYD
                     AAFYADDLDALNELSELVRDAECGVHIEPYALGNAA"
     gene            16270..16395
                     /locus_tag="ACHOWS_003203"
     CDS             16270..16395
                     /locus_tag="ACHOWS_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048736.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            16475..16963
                     /locus_tag="ACHOWS_003204"
     CDS             16475..16963
                     /locus_tag="ACHOWS_003204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048737.1"
                     /translation="MSINTPTEINKLFTDPAHIESTQKMLTEITNALDERISGIDKDN
                     HSSTYMTLKIQSMAMVIANQTISELYMKNLRLERELAELRAQMKTDESPTGEINARPE
                     HTNAHITMITRKAERTHICELLRELIETAKETHTCPQETCAAIERLIAILTNPDELKG
                     CA"
     gene            16963..17544
                     /locus_tag="ACHOWS_003205"
     CDS             16963..17544
                     /locus_tag="ACHOWS_003205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0048738.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFERFIDP
                     VDSERNGGTVNAATAVWWAGQSVDARACLRNADGTELAAVTEFLAFISRNMHDESPGK
                     TLTVWCKGASFDFPILKSAITRTTGDKSIPWCYWNERCMRPLIDMAESTGWRTPGRQG
                     KETIAHTALNDAIYQAKVVSEIWQRFTAPFLSV"
     gene            17582..18556
                     /locus_tag="ACHOWS_003206"
     CDS             17582..18556
                     /locus_tag="ACHOWS_003206"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFQ0048739.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYSRYILGDSNGALINFWRTARDNTDSLIDEAKDLFREHNNRTDYLAIRNTFNA
                     ENHGFLSTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGGYRTPYFPERE
                     IRAFADKANSTRTLLVHGDFRKTLSCASHLFSMGNTLCVYCDPPYLPFNGRDNFTAYG
                     KPFDEGEHVRLRAALDRLSQETGGMTSITISSSDTPEARRIYQGYRMNCIQAPRSVGA
                     RTREPAPEVIATLKQCNACGRHGGGYCPDCGPVTGYETYSAIFATANT"
     gene            18592..18828
                     /locus_tag="ACHOWS_003207"
     CDS             18592..18828
                     /locus_tag="ACHOWS_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048740.1"
                     /translation="MNSERNKTLLQGLRHIAHAHEETAKLATAVGKPTVVISSAISQG
                     ITIEAIQVIQDQEREIAALKKLLHGKSAGQGVKQ"
     gene            18825..19031
                     /locus_tag="ACHOWS_003208"
     CDS             18825..19031
                     /locus_tag="ACHOWS_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048741.1"
                     /translation="MIKESTVIRFTTHGHQYEVDESLIDQGMTRRDSRNPDIYHIRLI
                     NGSHFRATNVEEVRVLTTSCRRAL"
     gene            19368..19595
                     /locus_tag="ACHOWS_003209"
     CDS             19368..19595
                     /locus_tag="ACHOWS_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFQ0048742.1"
                     /translation="MANRKQQRARAERLHTRTEINRRLWRASRVAQIMYINMLHEHAH
                     ALSDRYCAAVFSYLADDLAELQDLINQQHHH"
     gene            19664..20080
                     /locus_tag="ACHOWS_003210"
     CDS             19664..20080
                     /locus_tag="ACHOWS_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048743.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINSTDSTAISWSSSEMALVLEMTTHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            20077..>21962
                     /locus_tag="ACHOWS_003211"
     CDS             20077..>21962
                     /locus_tag="ACHOWS_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFQ0048744.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVDFLTNTDEGKRINSHLHHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSAALERTCDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTAPAQEQVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMTCVRGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRKAINKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSPENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAALHRIHQFQFFGIPPRQV
                     WRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFATYITRQGGVLIPRNTYLVR
                     TAYET"
BASE COUNT         5775 a         5734 c         5063 g         5390 t
ORIGIN      
        1 actaataatc acgctaatat ttacctgttt gactgagttg ggattgcgtc gtttctccat
       61 taggagtaaa gctttaatgt cacctgaagt tcacagaata aggaacaggt tatgagctat
      121 agcaatattc ttgtcgctgt tgcggttaca ccggaaagtc agcaactgct ggcaaaagcg
      181 gtatctatcg ccaggccagt aaagggacac atcagtttaa ttactctcgc ttccgacccg
      241 gaaatgtaca atcaattagc tgcgccgatg cttgaagatt tacgcagcgt gatgcaggaa
      301 gaaacgcaaa gctttcttga taagttaatt caggatgcgg ggtatcccgt tgacaagacg
      361 tttattgcct acggtgagtt aagcgaacat attctggaag tttgtcgcaa atatcatttc
      421 gatttggtga tttgcggtaa tcacaatcac agtttctttt cgcgagcatc ctgctcggcg
      481 aaaagagtga ttgcctcaag tgaggtcgat gtgctgttag ttccacttac gggagattaa
      541 ccgctcctac gcaagcttag gaaaggtagc aactttatcg cgctgctggc tttccgcgct
      601 tcgcggaact atctgcttta ggtcgctaat gaagcactcc tgccagtggt taatatcgtt
      661 tttcacgata acgtccagca tttctgcatg acgggaaata cgttccgcca gcgacatagt
      721 caatgcacga tccagcgcag ctgcaacttc gtcacgatcg tagggattaa caattaacgc
      781 cgacgttaac tcgtttgctg ctcccgcaaa ttgcgaaaga acaagtacgc ccgggttggc
      841 tgggtcctga gcggcaacat actcttttgc caccaggttc atcccgtcac gcagtggcgt
      901 cactaagccc acgtcagagt agcggaatat tttcatcagt aatttacggt caaaatgctg
      961 attcaaataa taaagcggcg tccagcctaa ttgcccgtat ttaccattaa ttcgtccagc
     1021 ttcattttcg agctgatgac gaatatcctg ataggcttgc acatcaccac gcgacgttgg
     1081 tgcaatctgg gtataacgaa ttttaccatg atgctgcgga tatttttcca gcaacgcttc
     1141 ataggcgaga aaacgctccg gcaaaccttt ggaataatcc agccgttcga cagaaaagat
     1201 attttgtacg tttttcagtt ccgctttaag ttgcgccagt tttggcggca gtggtccggc
     1261 agcctgtttc gctatttctt tcggttcaat gccgatcggg taaacttccg ttcgaaacgc
     1321 tttgccgcag gccgtatggc tttttgcgct acgtgtcgtg acgcgggtca ggttagaaag
     1381 gcaatccagg aacgccagac gatcgttctc cgtctggaaa cccagcaaat cataatcaca
     1441 aagctgttca agcaaggtgt catatgtcgg cagcgcgttg aagatttccg gcgtcgggaa
     1501 aggaatatgc agaaagaaac caatgcgatt attcactccc cgtttgcgta attcatgcgc
     1561 aaagggcaac aggtgataat cgtggatcca gataatgtca tcgtcttgca acagcggcag
     1621 taatttatct gccagcaacg catttacgcg tagatagcca tcccaggcag gacgctgaaa
     1681 ttgcaccaga tcgagccgat aatgaaaagc gggccagaga acggcattgg agaatttgtt
     1741 gtagtattcg tcaaggtcct gttcgctgag gttaaaagag gcccacgtaa tgttaccctt
     1801 tttcaccttt tttagcggct gatcctcatt ccctgtttca ccgctccagc caaaccacag
     1861 tccacctgcg gctttcagtg cccccagtat gccaacggca aggccaccgg cactggcggc
     1921 gtgctcgtct ggtggtgcaa tccggttaga tactacgact aaacgactca tagtcatcac
     1981 tcctgttatt ttctcttttt tgttgtaatg cggtggttat catttcaagc cagctccaga
     2041 catccggcac gcccgccagt cgccatgatg cctgagttgc gcctgtgcca atttttactg
     2101 acattccgcc cagccggtta acgactgcga agccagattc atcggttaaa tcatcgccca
     2161 gaaatacagg cgttcgcccg ataaagggag cttcctgcat aaaggcagca atcgcctcac
     2221 ctttactggt gcctcgcggt ttgatctcga caacacactt tccctgctgt aacgccattt
     2281 gtggccagat ctgagtaata cgttgcgcta atgtcattaa tgcgtcttca tgctgcggag
     2341 cctgacgata atgcagcgca aaggccatcc ctttcgtctc cagctccgca ccgggaaact
     2401 gggcgatagc cgtatgcagt tgcacgctaa tatcacgcgc aatcgcatcc ggcagatgaa
     2461 cgatatgcgt tttaccattg atgtcacggc gctccgcccc atgcacgccc gctaacggga
     2521 agcgataagg ttttgccagt gcgtcaagct ccaccattga gcgccctgaa atcaatgcca
     2581 atgcaccatc acttgcggtt gccagtagct gaagtccttg cagaatagtg tcaggcacga
     2641 cgacctgatc gggatgcggt ttgatttccg ccagcgttcc atcgagatca aaaaaccagg
     2701 catatttcgc ggatagttca ggggtttcgg ttaacggttc tgtcacccgg ttctcctcct
     2761 tctcttttct tattgtgttg cgcagaacgc gctttacaga catgtaagta tagacagtgt
     2821 gacgggggtc gccatttgca acgaagaaca gccaaccgtt gtggcggctg gctgtggtgg
     2881 gaaaaaacgc taaattgttg tggaaaaaag catcagacag tgcgtttcgc tttttgcttg
     2941 taacggtcga aaatcaccgc tgccagcagg attaagccgc gaactacgta ctgtgcgaaa
     3001 ggagaaatat taagcaggtt catggcgttt tccacggtgc ctaaaattaa gatacccgcc
     3061 accacatatg agatttttcc gatgccgcct ttcagagaaa cgccacctaa aacgcaggcg
     3121 gagatcacaa tcagctcata gccaatcgac gtcattggct gcccactggt catacgtgaa
     3181 gccagaataa ttccggctat cgctgatacc aggccagaga gaacaaagat aataattttg
     3241 gtgcgaacaa ccggtacacc cgccagacgc gcggcctctt cgttcccgcc aatcgccagg
     3301 gtattacgcc caaaggtggt tttattcagc agcaagccaa agatgatcag acacgcgacg
     3361 gtgagccaga ttggcgcagg cagaccgaac cagttggcgt aaccaagggc aaagaagctt
     3421 tcatcttcga taccgaccgc tttaccgtct gaaatgatgt acgccagacc tcgaacaatc
     3481 tgcatcgttg ccagtgtcgt gatcagggca tttattttca gtttggcaat aacaaagcca
     3541 ttgaccaggc cacagagaac gcccagcaac aaacccgctg ccacgccaat ccacaggctt
     3601 tcagtcaggt taataaccac cgccgtggtg acacccgcac aggcaattac ggaggcgaca
     3661 gaaaggtcaa agtcaccgga agcaaggcag aacaacatgc cacaagccac catccccgac
     3721 atggaaattg ccagacccaa ccctttcata ttaataaagg tggcaaagtt cgggacaaaa
     3781 atggcacagg cgataaaaag gaccgcaaac accaccagca tgccgtactg atcccagata
     3841 cgccctaagc tgaatgacga cttaggtgcg ccagaacccg atgtagaaac agaagacatc
     3901 atactctcct tactcaggca actgcctggc tgactttagg catcgcaagg ctcagtgcct
     3961 gacgctcatc tgcctgctcg tgtaacaatt caccggcgat ttcgccttcc cgcatcacca
     4021 caatccggtc ggcaacgccg aggacttcag gtaagtcgct ggaggcaaac agcaccgcca
     4081 caccctgcgc cgccagcgca taaattacgt tatatatttc gtgcttagca ccaacatcaa
     4141 tgccgcgcgt aggttcatcc agcaaaatga ccttcatctc ttccgataac cagcggccca
     4201 gaatggcttt ttgctgattt ccgcctgaga gattcatgat cagttgatca gcgcccggcg
     4261 ttttgatgtt gagcgaacga atgtggtgat cggcattgtt ttcttcccaa ccgttgttga
     4321 ttacacaacc gccgagcaca tgtgtacgtc tggcactgat gttgatattg tcgcgaacgg
     4381 agtgcacggg aataatgcct tccgctttgc gatcttccgg gcagagcatc atgcctgcgg
     4441 caatggcgtg gctcggttta cgaatatcga tcggctgttg gtcgatataa acctgaccgg
     4501 cggtgatttg cgtaccgcca aacatgcctt tcattaattc gctacgccct gccccgacca
     4561 gaccaaacag accgacaatt tctccactgc gaaccgccag acttattggc gtacgcacgc
     4621 ctggtgcttt cacagcatca agacgcagcc gctcctcgcc ataactacgc ggttgccaac
     4681 cgtagatatc gccgatgtcg cgcccgacca tcgcctgcac cagggcgtcg tggtcaacct
     4741 gctgcatatc ggtaaaggtt ttgacataac gtccatcttt aaagacagtg atggcatcgc
     4801 tgagggcaaa tatttcttcc atacggtgag aaacgtataa gatcacccgc ccctcttttc
     4861 gcagttcacg aataacgcgg aaaagattgt cgatttcacg ggcagagagg gagctggttg
     4921 gctcatcaaa ggcgataatt ttggcattac gcgccagggc tttggcaatt tcaaccatct
     4981 gccactgacc aatggagaga tatttcagcg gcgtgtccgg gtcaatatcc ataccaagat
     5041 gtttaagttg taaacccgcc tcataattca gcaatgagcg attgacaatg ccgcctttat
     5101 gcggcagctg gccgagatag atgttttccg cgacggtcat ttccggcacg agatgcagct
     5161 cctggtaaat aatcgccacc cccgcgttaa gtgctgcggt cgtgtcggaa aaggacattt
     5221 cctgcccatt aatcactaca gaacccgtgg ttggcgcata gtttccgctg aggattttta
     5281 agagagttga ttttcctgcg ccattttcac ccatcaacgc atgaacctga ccggcatagc
     5341 agtcaaaact aatatccgtc agcgccttaa cgccgggaaa cgttttaccg atgccgcgaa
     5401 atgagagata cggggtagac tgttgcataa cgtctccgtg aatcactggt cgttcacacc
     5461 ccctctgcat catgcagagg ggaatctttc cggcaaatta cttaccgcct aaaccttttt
     5521 tctccagttc ttctttaaag ttgtcacgcg tgatcagcac cacgtcggta acttcggtaa
     5581 attttggcgg ttcaacgtct tttgctaccc agttgtaaag catttcgctg gacttataac
     5641 catgtacgtc cgggcttggc agcagggaac cgtagaagcc ggttgcctgt gccttagaca
     5701 gttcgctcac cgcatccaca ccgttaatac cgataccgat gatatcggcc gctttaaagc
     5761 cctgaccttc cgtcgcgcgt acgccgccca gcacggtgct gtcgttcata ccgacgatca
     5821 gccaatgttt aacttccgga tgttgaacca gcattgagtt ggcagcgtca aatgcccccg
     5881 ggatgtcgtt agatttggta ggtacctgat aaatttgttt ttccgggaat cctgcagctt
     5941 tcagtgcgtc catagaaccc gttgtacggc ggcgggcggt atccagttcg ttggcggtaa
     6001 tcgccatcac cgcgctttct ttgacatccc agccacgttt ctgcatctct ttatacagtt
     6061 cctggccctg acgttcgcca attttggtcg cagccatcat caccagcgga acggtatcca
     6121 ttggcttacc tttggcgtta acaaactggt catccacggc aatgactttc atatcgtagc
     6181 cacgcgcttt cgccacgatg gcagagccga gtttcgggtc cggagtacaa ataacgaaac
     6241 ctttcgcgcc actggcagcc aggctgtcga tagcgttcaa tgttttttcg ccatccggca
     6301 cggcaatctt aataacttca aaccctaaat ctttcccggc tttatcggca aacttccatt
     6361 cggtctggaa ccacggctct tccggttgct tcaccaggaa accgagcttc aggttctccg
     6421 ccatagcgga ttgtgacata acggctgcca gaccaatggc tgccagggct ttagtaaatt
     6481 tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag ggcaaaaacg aatgacattc
     6541 gttaaattaa tcggaaaaca aagcattacc ttttaactaa aagataagtg actgtgttga
     6601 catagtttta gcgagaaatt aattctccat aggagagcaa tatcacatcg cagaattaca
     6661 gtgagaacgt gcataaattt agcgggaaaa gacataagga aaagcgaatt tgtcagacaa
     6721 attatcgagt gcacagcaga ataatccata agattagcct ggaagtcctt gttgtctttg
     6781 gtacccatgc gggatgtctt ctttttaacc agtcaatagg ccgcattacc tggcgttgag
     6841 tttttgcaat ggtgtaataa ccacaactca aagatgtgga aaatgtgcgt cattcatttc
     6901 gccattaatt atcactgtgc tcattaatta acagaacacg tataatgaga gccatctcgc
     6961 aaaaatgaaa aaacgtttta taaaaccatc acttcatcat gaattcaaat taatcgatta
     7021 atatcaacaa gatacaaaaa gcactatcat taaaattcat tgcagttaca ttgatttcat
     7081 caatgaaatg taaaaatata taaacttgat gatttaagca ttttcttata cccgttcaga
     7141 cgttattctt atttcagatc atcgtcagaa ttgactccac gatcacattt cggaccggca
     7201 gaaaggaatt attctgcaaa cagtaattat ggtgttttga tttatcttgc acctctccac
     7261 ttctggatat aaggatatta ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc
     7321 aaatgaaccg cgagttttac gcatccaatc tctaccttca cctgagtaac tggtgttctg
     7381 aacagagtct gaacggcacc gccactttcc ttcgcgccca ggcacagagt aatgtgaccc
     7441 aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc tacccccatc gttaaagcca
     7501 ttgatgttcc cggtgaaaaa ctgaactctc tggaagaact gttccaaaaa acgatggaag
     7561 aatacgagca acgttctagt acgttggcac agttagccga tgaagcgaaa gaactgaatg
     7621 atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga acagcagcat gatggcctgt
     7681 tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact tgcgggtatg tgccctgtgc
     7741 agaccgacca acatgttctg aatgtcgtgt cgcatcagct acattgatca tcatcggcgc
     7801 taatgcattg cgccgatgaa gtttttgaga aaccgctgcc tcattttttt gaagcagcgg
     7861 ttttttaatg ggattcgccc tgtggggtaa acttgagttc aatcaacgcg atggcttttt
     7921 ggattgcccg catggtgacc gggtctgcgg cggcgggatg gttagtaaag tcgatattct
     7981 ttagctggct ggacattttt tcgcgaactt caacgggtgc gatcacatcg agaacatcca
     8041 gaatttgttt gataaccaac tggcaagcaa ccacatcaga aaccagttcc tgatcggcat
     8101 tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat agtagcggct
     8161 tcacatcttc agcgatagtc acatccaccg tcatcaggac acaaaaaaac ctgccggagc
     8221 aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt
     8281 tattgaacat acttttcagg attttgcgca gtttcatcgc ggcactccga ccatttgtta
     8341 tacaggtgtt attgtctttg cgcttataat gtgacaacca tcacaaaaat caatctttat
     8401 gtgatgcaaa tcacataaat acccctttaa tgttataaaa atgataatca aaaagcagct
     8461 accccatttc tgacacctac agatggcaag aaatagcgcc tgccaggcgt cttttccggc
     8521 cattgtcgca gcgctgtagc gcgtaaaata gtgctttctt ttactcttct ggctggacca
     8581 tgagacttct gattctgact ctttcattaa taacgctggc ggggtgtacg gttactcgcc
     8641 aggcccatgt gagcgaagtt gacgcggcaa caggtattgt tcggttggtt tatgatcagg
     8701 cttttttgca gcatgcccgt accgatcgtt atgtcagtcg cgggatcgcc gatcgcgcat
     8761 gccagcaggc aggctatacc cacgctatcc cctttggtca gccagtaggc aactgtagcc
     8821 tctttgcggg ttccttatgt ttgaatacgg aattcacttt gtcatatcag tgccatcatt
     8881 ccgccttccc tgtctttctc taaacgtttt aatcagcgag ggggatcttc gctgattaaa
     8941 taaatagcgg taatgcgttt tattcccatt cgtattttta ataaatgatg tttatatttt
     9001 accttttgca aataataaaa taacaaatta tagtgacgcc acagcaacaa atataacctt
     9061 tgtggagcac tatcatgctg aaaccagaaa tgattgaaaa acttaatgag cagatgaacc
     9121 tggaactgta ctcttcactg ctttatcagc aaatgagcgc ctggtgcagc tatcatacct
     9181 tcgaaggtgc tgccgcgttc ctgcgccgtc acgcccagga agagatgacg catatgcagc
     9241 gtctgtttga ttacctgact gataccggca acttaccgcg tattaatacc gttgaatctc
     9301 cgtttgctga atattcctca cttgatgaat tattccagga aacctataaa cacgaacaat
     9361 taatcacgca gaaaattaac gaactggctc atgctgcaat gaccaatcag gactacccaa
     9421 catttaattt cctgcaatgg tatgtttctg agcagcatga agaagagaaa ctgttcaaat
     9481 cgattattga taaattaagc ctggcaggca aaagtggcga aggtctgtat tttatcgaca
     9541 aagaactctc taccctcgac acacaaaact aatgctctcc ggcggcaggt tttctgccgc
     9601 cgtttcaaat cagtgacggc aaatcttact ctgatcggtt gaaaaacctt cttctgaagg
     9661 tataaatttg ccttttgctg ccagaaacgc caccaactct cccgcagtca tcccttctgc
     9721 cgagcaggtg tgaaaacgtg cctgttcacc aaaacgcgct ttaattgctg cttccagact
     9781 ggcatgcgta tattgctcgc ctgattcaat catcatattt aacacttcgt gaccgtgaat
     9841 agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg attgccgctt atgaagactt
     9901 tgcgccagcg cagaactgaa cgcttttatt gtacatttat atttacatca tatgtaacgc
     9961 cggtttgacg aatcagccgt tatgccttaa cctgcgccgc agatatcact cataaagatc
    10021 gtcaggacag aagaaagcgt gaaaaacaga actctgggaa gtgtttttat cgtggcggga
    10081 accacaattg gcgcaggcat gctggcaatg ccgctggctg cggcaggtgt tggttttagc
    10141 gtcacgttaa tcttgttaat tgggctttgg gcgttgatgt gctacacggc gctattactg
    10201 ctggaggtgt accagcatgt cccggcagat accggtctgg gcacgctggc aaaacgctat
    10261 ctgggacgct acggtcagtg gctgacgggc ttcagtatga tgttcttaat gtatgctctg
    10321 actgcggcat acatcagcgg tgccggtgaa ttgttggcct ccagcatcag cgactggaca
    10381 ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca cttttgttgc cggtggcgtg
    10441 gtttgtgtcg gaacttcgct ggtcgattta tttaaccgtt ttctgttcag cgccaaaatt
    10501 atttttctgg tggtgatgct ggtattgctg ctaccgcata ttcacaaagt gaatctttta
    10561 accctgccgt tgcaacaggg actggcactg tctgcaatcc cggtgatttt tacgtcgttt
    10621 ggttttcacg gtagcgtgcc gagtattgtc agctatatgg atggcaacat tcgtaagcta
    10681 cgctgggtgt ttataatcgg tagtgcgatc cccctggtgg catatatttt ctggcagctg
    10741 gcgacccttg gcagcattga ttcaacaacc tttatgggat tgctggctaa tcatgccgga
    10801 ttaaacgggc tgttacaggc gttacgcgaa atggtggcct ctccgcatgt tgagctggca
    10861 gtgcatttat ttgctgattt agccctcgcc acgtccttcc tcggcgtagc gttaggctta
    10921 tttgattatc tggcagattt gtttcagcgt tcaaataccg ttggtggacg attgcaaact
    10981 ggtgcaatta cctttctgcc gccactggcg tttgcactgt tttatccacg aggatttgtg
    11041 atggcgctgg gttacgccgg tgtggcgctg gcggtactgg cattgattat cccttcgctg
    11101 ttgacctggc aaagcaggaa gcacaatcct caggcgggtt accgggtgaa aggtggtcgt
    11161 ccggcgctgg tggtggtgtt tctctgtggt attgctgtga ttggcgtgca atttttgatt
    11221 gcggcaggct tgttaccaga agtggggtga ttagattctt attgggtgcc agaattaacg
    11281 ctgacaccca atttgacctc ttaatgcagg cagcactgct taaatttctt accactaccg
    11341 cacggacaag gatcgttacg ccccggtttc tcttctgctt tgataggttg ctgaacagct
    11401 ttttcctgcg gatgcgccat ccagtatgca tgtaaatcaa gcgccgccag tcgaatagca
    11461 tctacgctct cttcaaacgc ttctggcgac atcttctcta cccgctcgaa gttttcctca
    11521 gtaccgtgca gcgcaatcgc atccagcgct ggttttaacg aatcgggcaa cgttgaccag
    11581 tcagaaagtg ccacgccccg catatagcca aagcaccact cctcaacaat cgtcagctcg
    11641 ctgccatcga cttctcgcaa gccgaataac ggctcaaact gctccgggaa ttcgttcaga
    11701 cgctctgcgg tatcagccat atgttgaaaa gccaggttca taaagcgcgt catctctttc
    11761 tctgacgccc agcgcggcac atagtcagcc ccaccccaca cggcaaccag ccactgtgcc
    11821 gattcaatct cttgcggaga actcaacacc gccgtcaata aaccgtccag ctccgccaca
    11881 tcaagaatgg cgtggtcagt gttgtatttg gtcagaatat cgtccagcca ttccaactca
    11941 ctttcgttta acggtcccgt tttcatacgc ttttccttgt ggatctcaac tcgccagcac
    12001 ctatcttaca tgccggtccg tatcagagat actttttgag tggctttgcc ggtgattaaa
    12061 aattaaggag ggtgtaacga caagttgcag gcacaaaaaa accacccgaa ggtggtttca
    12121 cgacactgct tattgctttg attttattct tatctttccc atggtacccg gagcgggact
    12181 tgaacccgca cagcgcgaac gccgagggat tttaaaaact atcaaccacc atttataaat
    12241 cataacgtta tgattttatt aagtataaag ataaagcaat atggcgctat ttgatgcttt
    12301 tgttactttt catcgccact ttttaccctc tattgtcaaa gggattcagt tcaacagcag
    12361 cctctaaatg acccggcgca aagtgtgcat aacgcatagt catttttata tcgctatgcc
    12421 ccagtatttt ttgcaacaca aggatatttc ccccccgcat cataaaatgg ctggcaaacg
    12481 tatgacgtag cacatgagtt aattgcccat caggaagttc gatctttgct ctcttaatag
    12541 cggtgtcaaa agcctcataa catggtgaaa aaagcgcccc gcgtttttta ggaagcatgg
    12601 cctgcaattg aggtgaaatc ggtacagtgc ggttcttctt tcctttggtt ttaacaaatg
    12661 tgattcgtcc gggcagtatt tgagattgct ttaatccctc tgcttcactc cacctggcac
    12721 cagtcgcaag cccaatacgg acgacaacac ccaaatcttt attccgtgac tcatcacatg
    12781 caatcagaag gcgttcaatc tcatctgcat acagaaacgc cagttccttt tcttcctcac
    12841 gaaacttgcg aatacctgtc aatggatttt caccagacca ctccccaagg cgcttcagtt
    12901 cagcaaaaac agcatgtaaa tatgactgtt cgcgattaac ggttgcttca ctaagttttt
    12961 tcttcccctt tggattccat tcacctgata atcgccgttc cctgtagatg gcaaacatat
    13021 ttttatcaaa ttgagaagca aacggatcac ccaacctaga acaaatcgcc tcaagtttag
    13081 cctttcgctc ttcaccggaa gacaacgttt taccgtacat ttcaaaccag cgagcaacca
    13141 actcggaaag acggggacca gaaccatcct gaaactcgcc ccctaaatta ctgttcatta
    13201 aacggcgctc ataagagagc gcctcacctt ttgtagcaaa ctgcttacga atgcgttttc
    13261 ctgctgctcc gtagggatag cattcacaga gccatttacc agtagatagt ttacgaacag
    13321 acatattaaa aagtgcacac caaggaaaat tttcgttatt aagatgattg ctcaacccgc
    13381 ataaagagct tttcatcaac taactgctcc cattcatcag gatcaagctc ccaagaagga
    13441 gccgacatat catcctcatc cccctcattt acaaaagtaa ccaaatagaa aggaagatct
    13501 ccgggctcat cgtcatcgtc caggacaata tgaacatcag taacgactat acgattagat
    13561 ggattaactt tagatacata taaaccagtt aatggaattt gcggattagt taccataatt
    13621 catttacctc tttggacttc ctcatgaata aagacttact tttctttaag accatgacaa
    13681 gcaactcaca ccatttctta catcactagt tgccccctct cacctttgtc agctcgctta
    13741 atttatcttc ttcatttctt ttaaagtcgg taaagcaagc ttttgccatg atatctttct
    13801 ccaacaacta tctatcagaa aagaataatg cctaataaaa caccaataac gaaacacatt
    13861 cctgtttctt ttgggtattt tttaattatt tgctgaaaat tcatctgtaa ttgactatcg
    13921 gcagaattgt tggcagttaa tttcttttct ccttccacag attgttggtc taaccaggaa
    13981 agagccatct gaagctgaga acgagtaaga tcgtttaagc gacccgttcc gaaattgata
    14041 tggcaatagc ggataagttt ttgacgaagc acgccatctt cactattacg aagtaaaata
    14101 cttacaagtg ccttacgcgc atcatgatct ttacatcgtt caagcatcgc acacagaaaa
    14161 ctctctgcgg tcttatattg attcattgtt atatcatcaa tactgtctac accaatctct
    14221 gcatgcaatt tttgccaaat aataaacgcc tcggtattgc tggcatctac aatagtagca
    14281 actaagctat ttaatgcctt ccgttgtccc ttaactaagg ggcgaggatc actgtcattg
    14341 ctcgaaggaa ttgcgatatt gacggtatga gagccatcat atcgttctat ctgaatattc
    14401 ttttcttggt aatcccgccc agcaatacga ttgtctgaac cgtttgagtt gaccgccatg
    14461 ccacctcctt gctatcactt accctttgtt tcgttatagt cacgacctgc gatgcggtta
    14521 ttaccgccag aaatatttaa ccgcccctca gatgacagag aatccttttc actgactgca
    14581 cctttcaaag caccaatcac ggcgtttttc acatctagtg aggcactacg aaaacggctg
    14641 atcaactcct gctcatcatc gttataagtt tcaggtgaat gaattcccaa cacgacatac
    14701 tgaacatcaa ggccaaaacg cgacaatgct gataagtacg ccgcatcagg aaagctatcc
    14761 cctttctcat atctaagttg ggttaacttt ttaaccccac caatatcact catagcgact
    14821 tggctaagac ccagccgttc cctttcctca cgcaaccgcc gcccaatatc atttttcata
    14881 caaaaacctt gacatgtatc ttttttgata ccaaaatact ttcactagcc attagatgat
    14941 cacaatatac cactatgaaa caagttgttc acgacaccag atcacgtatt ccgcgtaaca
    15001 cagctacagg acagcgactg gcacttcgat tgtcagcaga agagcgagcc atcattgatg
    15061 aaatggcagc taaagaacag cgctcatcct ctaacatggc acgcatgatc ttccttcgcg
    15121 gccttgagat tatccagaaa gagcaaaacc aatcttcatg ctcaggaggt taacgggatg
    15181 caaggtataa caataaacat caacgttaat gccccttacg tatctctgaa gaaatacgcc
    15241 gagataacag gcattcctct taacacatgc aaaaagatgt tggctgacgg tcgaattatt
    15301 atccgaccca agcgcgcaag aatggaaaag ccagaagtaa accttatagc gatgttaaaa
    15361 gacgcaatag ccaacagcta aacaatgagg cgagcagaac tatgaagaaa aatgctattc
    15421 ctcccagacc taaacaccgc actggcacag aacgccacgc taaccgtttc tcaaccagcg
    15481 catcacgcag caactcgcac tacagcctgc aggaaacgca cgccacacct gacgggcatc
    15541 tggttaaaca aaccggcaaa cacacctggc tgattaatga tgccggaatt gtcatacaca
    15601 gaggcgcacg caacccgatt accggaaata cctgttactc cctgactcgc ggagatgagc
    15661 agttcgggca ggatttcacc ctgcacgaag cattaagaac ggcagaccgg ttaatcagcg
    15721 gtcgcagttt tcagcatcca tgggggaatt aatacatggc aacaacgaca aaagacatgg
    15781 cagagatagc atcagcctac acaattatca tacaccggct catagctaat aatgcccgta
    15841 atgcactaaa tacagcaaaa cctttatggg aaacaaaaaa agatatcatc agcgcattac
    15901 aggggctaca gaaatgcgcc gacctcgcag gcgattacgc cgtatacatg gctattgaag
    15961 acacgataga gaagattgaa tcaggtaaat cattaagcga ttttgtttaa ttgttttccg
    16021 gggaaataca atggacaaag aatataaggt tctcatcaat aaagcccttg agcgctttaa
    16081 ctttcgcctg agtatgtcag gagttgccgc agaacatgca gcaagagact cactgaccag
    16141 agcaattcgc agcctgtatg acgcagcttt ttacgctgac gatctggatg ctctcaatga
    16201 actgtctgag cttgttcgtg atgcagaatg cggagtacat attgagccat acgcacttgg
    16261 gaatgcagca tgagcgtatt tctttcatgg gtgattctga ttatttccgt gcttatggcg
    16321 attggcatta tgcgaattat cgaagcgctg aaaaatatcg aatctttatt tactggcgaa
    16381 gaacgccgta aataaataac caaccggaca gaaaacatta aaaccatccg catctgcgga
    16441 gggcttcact caatccagaa acggagaaac agccatgagc atcaatacac ctacagaaat
    16501 aaacaaacta tttaccgacc cggcgcatat tgaatcgacg caaaaaatgt tgactgaaat
    16561 aaccaatgca ctggatgaaa gaatatcagg aatagataaa gacaatcatt cttctactta
    16621 catgactctg aaaatacaaa gcatggcaat ggtgatagca aaccagacca tcagtgaact
    16681 ctatatgaaa aacctgcgcc tcgagcgcga actggcagaa ctccgggcac aaatgaaaac
    16741 agacgaatcc cccaccggag aaataaacgc cagaccggaa catacgaacg cgcatatcac
    16801 catgatcaca agaaaagccg agcgtactca tatttgcgaa ttactgcgcg agctgattga
    16861 gacagcgaaa gagacacaca cctgcccaca ggaaacctgc gcagccattg agcgactgat
    16921 tgcaatactc accaatccag acgaactgaa ggggtgcgca taatgaacca tctcatgatt
    16981 gacaccgaaa cactgggtaa cggaccggac gcagccatat tcgcgatcgg ggcagtgttt
    17041 ttcgacccgt tcaccggaaa gctgggaaaa cagtttgagc gattcattga cccggtggat
    17101 tcagagcgca acggtggaac cgtcaatgca gcaaccgctg tatggtgggc cggacaatcc
    17161 gtggacgcca gagcctgcct gcgcaacgca gatggaacag agctggcagc agtgaccgaa
    17221 ttcctggcgt tcatttcacg caatatgcat gacgaaagcc cgggcaagac actgaccgta
    17281 tggtgcaagg gcgcttcgtt tgacttcccg atcctgaaat ccgctatcac gcgaacaacc
    17341 ggagataaaa gcatcccctg gtgttactgg aacgagcgct gtatgcgtcc gcttatcgac
    17401 atggctgaat caacaggctg gagaacgcca ggcagacaag gaaaagagac cattgctcat
    17461 accgcactga acgacgccat ttaccaggcg aaggttgttt cagaaatctg gcagcgcttc
    17521 actgcgccat tcctgagcgt atgaagcgga cacacgaaac ctgtaacaaa gaggccgcgt
    17581 tatgagcaaa gaactgttat caggcacacc actgaaatgg gtgggcggaa aaagccgcat
    17641 catgaacaca ctgaaggcgc acctaccaga agccgactgt ctggttgagc cgttcgtggg
    17701 cggcgcatcg gtattcatga acaccagtta cagccgctac atccttggag attccaatgg
    17761 tgcgctgatt aatttctggc ggacagcacg ggacaacacc gacagtctga tcgacgaggc
    17821 aaaagacctg ttccgggaac acaacaacag gactgactat ctcgccatcc gaaacacctt
    17881 taatgcagag aatcatggat tcctgagcac gggaataagc gaccgtcggg aactgcgact
    17941 ggccgccatg tttttgtatc tcaaccggca ctgctttaat ggcctgtacc gggtgaacgg
    18001 aaaaggtgac tttaacgttc ctttcggtgg ctaccggact ccctacttcc ctgaacggga
    18061 aatccgcgca tttgcagaca aggcaaacag cacccgaaca ctgcttgttc atggcgattt
    18121 tcgaaaaaca ctgagctgtg ccagccacct gttcagcatg ggtaacacgc tttgcgtgta
    18181 ctgcgatccc ccttacctgc ctttcaatgg ccgggacaat ttcaccgcct acggaaaacc
    18241 attcgatgaa ggcgagcacg ttcgcctgag agctgcactt gaccgccttt cacaggaaac
    18301 gggcggcatg accagcatca ccatatccag ttccgacaca ccggaagccc ggcgtattta
    18361 tcagggttac cggatgaact gcatacaggc accacgaagc gttggcgcaa gaacccgcga
    18421 accagcaccg gaggttatcg ccaccctgaa acagtgcaac gcatgcgggc gccatggcgg
    18481 cggctactgc ccggactgcg ggccggtgac gggatatgaa acgtacagcg caatatttgc
    18541 cactgccaat acgtgaataa acagatgaat ccttaacacc ggagtcaacc aatgaatagc
    18601 gaaagaaaca aaactctttt acaggggtta cgtcacatcg cccatgcaca cgaggaaaca
    18661 gccaaacttg caactgccgt tggaaaacca acggtcgtta tctcttcagc gatttcacag
    18721 gggatcacca tagaagcaat tcaggtaatt caggatcagg aaagagaaat cgccgccctt
    18781 aaaaagctac tccacggtaa atctgccggg caaggagtca agcaatgatc aaggagtcaa
    18841 ccgttatcag attcacaacc cacggccatc agtacgaagt cgacgaaagc ctgattgatc
    18901 agggcatgac acgccgggat tcacgcaatc ccgatatata tcacatccgc cttatcaacg
    18961 gttcgcactt ccgcgccacg aacgtggaag aagtgcgcgt gcttaccaca agctgccgtc
    19021 gtgctctgtg aactgttacc gggctgagtg atcatcatgc acacacaaaa aaaccgcttg
    19081 ccagcgttcg tctggtcggg ttacagtttt cccgcacctc ataaaacggg tgccgggttt
    19141 cgcagcctgc cgactacaag agcgcacaac cgcgccagcg gtttttttgt gcgtactgta
    19201 tcgccacgtc tttttcgcgt cagaattatg gcggggcgta cggggccgac ttcggtcggg
    19261 ccgggatctc ttgtagccgg tactgcgaac ctcgtacgtc tcgccaccca cagtttcgca
    19321 gctctggatg gtgagttttc agaacttact acaagaggcc aaacaccatg gcaaaccgca
    19381 agcaacagcg cgcccgcgct gagcgtcttc acacccggac tgaaatcaac cgcagactct
    19441 ggcgcgcatc acgcgtcgcg caaatcatgt acatcaacat gctgcatgaa cacgcacacg
    19501 cgctgtcaga ccgttactgt gccgccgtct tcagctatct ggcggatgac ctggcagaac
    19561 tgcaggacct tatcaaccag caacaccacc actaaacaac atcctgaagc catcccgcac
    19621 ccgtgcgcgg gaggcttccg cacgtctgtt accggaggtt ctcatgaaga gcgttaaggg
    19681 actttcagat attcaggact tttttaactg gatgtgggca gtggatcccc ggctggcttc
    19741 ccgcctgaac gactggcacg actattaccg ccgcgcctgt aaacaggccg ccagacgcgt
    19801 caccacatac ggggatttct ccttcaccgt tgacgaccgt taccgcgtgg acgtgaaagg
    19861 cgatggcgat ggctttggtt tatttcacat caacagcacc gacagcaccg ccatcagctg
    19921 gtcgtcgtca gagatggcgc ttgtgctgga aatgacaacg cacagcgtgg ccgtgaaagg
    19981 catatacacc gcagaagact ttatcggtga ataccaacgc cttctcgtgc tgtaccgcgc
    20041 ggcgtttcag gcggcgactg ccggggagtg cgacgcatga gtgaccacaa tgcacagctg
    20101 gcgtacccgt ggaacgcccc gctaccggtt atcgatcctg aaacgttcgg cagagcgcaa
    20161 ccaaccgcat tacgccaggc cattcagagc tacatcaacg aagacattcg cattgatgca
    20221 agactggatg aagagaccgt cgacttcctg acaaacacag acgaaggcaa gcgaattaat
    20281 tcgcatcttc accacgacga agagcgtcgg atcaggctgg aaaaactggc gcggcacaaa
    20341 cacgagaatc cgccaacact ggtatctgaa gccatggcag agctgcgcgc ccttccgccg
    20401 tttcttgccg gtccgctcat ccgtgacctg gcacgcctga gccgcaaaca ggagagcgca
    20461 cgccgggaag ggcttaagaa caacagcgca tacgcggcgg ataacttcgt tcgtcacggg
    20521 ctgcgtgccc gcctgaaacg tatcgcccgt gtgaatgaac gctttgccac cccggccttt
    20581 aaggccaccg ccgcgcgcga acggctggat gaactgctta tgctgccgca gctgagccgt
    20641 gacgaaatcc agcgccttgc caccctgacc gccaccgcgt tttccgccgc actggagcgc
    20701 acatgtgatg aggtcatcga aaagaccggc aggggtgacg atgacctgtt cacctggtta
    20761 ctgacctacc agccactggc acacatggcg ttaaaactgg gtatcacgcc accctgctgg
    20821 ccgtcgctgg aaatcaggcc cgaccgccgc accgcccccg cccaggaaca ggtgccgggt
    20881 gcggtcatgc gcctttcctg tgcgacatgg tggaacagtc agttaagaca cctcagggac
    20941 atctggcgtg aagagctgtt acgcgccgca ggtctggtgt cacgtaagac atcggtatat
    21001 atcagccatg aagcgttact ggactggcgc gaaaaacgcc agcgcacccg cgattttctg
    21061 aaggcctggg acatcgagaa cgaagaggga gaacggttca gccttgagga tatctactgg
    21121 gcaggtctct ctaatccgcg taaccgccgt aacgagatga tgacctgcgt ccggggaatg
    21181 gagcaggtcg ccgaagcacg cggcgacgtg gcgtttttcg tcaccgtcac agccccttcc
    21241 cgttttcaca gcgtgaacga tgacggcagc ctgaacccga aatactgcgg agcaacggta
    21301 cgcgatgcca gtgattatct ggtttaccgc tttttcgccg ccgtccgcaa ggccatcaac
    21361 aaggccgggc tgggctggta cggcgtgcgc acagtagagc cccaccacga cggtacaccg
    21421 cactggcaca tgctggtatt caccagcccg gaaaacgaag cccgtatcac tgaaatcatg
    21481 cgtaacgccg ccatcaggga ggaccgcgca gagctgggtg atgatatctc cccgcgtttt
    21541 aaatgcgaaa aaatcgaccc ggcaaaaggc acaccggcaa gctatatcgc gacctatatc
    21601 ggtaagaacc tggacgccag tgccttcatg gggaatgacc cgaaaaccgg caaaccttac
    21661 gtcgataagg aatcaggcaa aaccatggcc gaaaccgtgg agaacgccat cggctgggct
    21721 gcccttcacc gcatccacca gttccagttc ttcggtatcc cgccccgcca ggtatggcgc
    21781 gaactccgcc gccttgccgg gcagatggcc cgtaacccgg ctgccccgca gcgtctggac
    21841 catgacgaca ttgacgccat actggccgcc gccgacgtgg gatgttttgc cacctatatc
    21901 acccgccagg gtggtgtgct cattccccgc aacacgtacc tggtccgcac cgcatacgaa
    21961 ac
//
LOCUS       DAZKJR010000084        21951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZKJR010000084 DAZKJR010000000
VERSION     DAZKJR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.084"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(254..1537)
                     /gene="gltA"
                     /locus_tag="ACHOWS_003212"
     CDS             complement(254..1537)
                     /gene="gltA"
                     /locus_tag="ACHOWS_003212"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFQ0048745.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1743..1850)
                     /locus_tag="ACHOWS_003213"
     CDS             complement(1743..1850)
                     /locus_tag="ACHOWS_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048746.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2231..2635
                     /gene="sdhC"
                     /locus_tag="ACHOWS_003214"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2231..2635
                     /gene="sdhC"
                     /locus_tag="ACHOWS_003214"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFQ0048747.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2629..2976
                     /gene="sdhD"
                     /locus_tag="ACHOWS_003215"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2629..2976
                     /gene="sdhD"
                     /locus_tag="ACHOWS_003215"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFQ0048748.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2976..4742
                     /gene="sdhA"
                     /locus_tag="ACHOWS_003216"
     CDS             2976..4742
                     /gene="sdhA"
                     /locus_tag="ACHOWS_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFQ0048749.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASASDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4758..5474
                     /gene="sdhB"
                     /locus_tag="ACHOWS_003217"
     CDS             4758..5474
                     /gene="sdhB"
                     /locus_tag="ACHOWS_003217"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFQ0048750.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5775..8576
                     /gene="sucA"
                     /locus_tag="ACHOWS_003218"
     CDS             5775..8576
                     /gene="sucA"
                     /locus_tag="ACHOWS_003218"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFQ0048751.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8591..9808
                     /gene="odhB"
                     /locus_tag="ACHOWS_003219"
     CDS             8591..9808
                     /gene="odhB"
                     /locus_tag="ACHOWS_003219"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFQ0048752.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPVKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9902..11068
                     /gene="sucC"
                     /locus_tag="ACHOWS_003220"
     CDS             9902..11068
                     /gene="sucC"
                     /locus_tag="ACHOWS_003220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFQ0048753.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11068..11937
                     /gene="sucD"
                     /locus_tag="ACHOWS_003221"
     CDS             11068..11937
                     /gene="sucD"
                     /locus_tag="ACHOWS_003221"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFQ0048754.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12041..12763)
                     /gene="mngR"
                     /locus_tag="ACHOWS_003222"
     CDS             complement(12041..12763)
                     /gene="mngR"
                     /locus_tag="ACHOWS_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFQ0048755.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12933..14849
                     /gene="mngA"
                     /locus_tag="ACHOWS_003223"
     CDS             12933..14849
                     /gene="mngA"
                     /locus_tag="ACHOWS_003223"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFQ0048756.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALNLKRSDETRTVQQDT
                     QPVKSLKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14867..17500
                     /gene="mngB"
                     /locus_tag="ACHOWS_003224"
     CDS             14867..17500
                     /gene="mngB"
                     /locus_tag="ACHOWS_003224"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFQ0048757.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLTFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKLWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLHERSARQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREYEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(17645..19042)
                     /locus_tag="ACHOWS_003225"
     CDS             complement(17645..19042)
                     /locus_tag="ACHOWS_003225"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFQ0048758.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEAHKDVIAQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     CVSTLPSEGLWIRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALQATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRHWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGNLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRFEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
     gene            complement(19432..19917)
                     /locus_tag="ACHOWS_003226"
                     /pseudo
     CDS             complement(19432..19917)
                     /locus_tag="ACHOWS_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
     gene            complement(19936..21324)
                     /gene="glmL"
                     /locus_tag="ACHOWS_003227"
     CDS             complement(19936..21324)
                     /gene="glmL"
                     /locus_tag="ACHOWS_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFQ0048759.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQSYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSASANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(21324..21773)
                     /gene="glmS"
                     /locus_tag="ACHOWS_003228"
     CDS             complement(21324..21773)
                     /gene="glmS"
                     /locus_tag="ACHOWS_003228"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFQ0048760.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         5282 a         5467 c         5885 g         5317 t
ORIGIN      
        1 tattgtttaa tgagatgaaa gatggggtgg gatggatatg cataattttc attgggttaa
       61 caatgctacc ggcaggaaag taaaaaaata aacttatcat tctgctcagt atgtgacttg
      121 tcacattttt tcaccttatt aatgggtatt tgcatctggt ttaattttta caatttatta
      181 tgttcagcca tataaaaaag aacccgctct attaaaggcg ggtccggaaa gtaaacggct
      241 tagcaatcaa ccattaacgc ttgatgtcgc ttttaaagtc gcgtttttca tatcctgtat
      301 acagttgacg cggacgggca atcttcatac cgtcactgtg catttcgctc cagtgggcga
      361 tccagccaac ggtgcgtgcc atagcgaaga tcacggtgaa catagaagac ggaataccca
      421 tcgctttcag gatgatacca gagtagaaat cgacgttcgg atagagtttc ttctcgataa
      481 agtacgggtc gttcagcgcg atgttttcca gctccatagc cacttccagc aggtcatctt
      541 tggtacccag ctctttcagc acttcatggc aggtttcacg cattacggtg gcgcgcgggt
      601 cgtaattttt gtacacgcgg tgaccgaagc ccatcaggcg gaaagaatca tttttatctt
      661 tcgcacgacg aacaaattcc ggaatgtgtt taacggagct gatttcttcc agcattttca
      721 gcgccgcttc gttagcacca ccgtgcgcag gtccccacag tgaagcaata cctgctgcga
      781 tacaggcaaa cgggttcgcc cccgaagagc cagcggtacg cacggtggag gtggaggcgt
      841 tctgttcatg gtcagcgtgc aggatcagaa tacggtccat agcacgttcc agaatcggat
      901 taacttcata cggttcgcac ggcgtagaga acatcatatt caggaagtta ccggcgtagg
      961 agagatcgtt gcgcgggtaa acaaatggct gaccaatgga atacttgtaa cacatcgcgg
     1021 ccatggtcgg cattttcgac agcaggcgga acgcggcaat ttcacggtga cgaggattgt
     1081 taacatccag cgagtcgtga tagaacgccg ccagcgcgcc ggtaatacca cacatgactg
     1141 ccattggatg cgagtcgcga cggaaagcat ggaacagacg ggtaatctgc tcgtggatca
     1201 tggtatgacg ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc ggtttttcac
     1261 cattcagcag gatgtaacaa acttccaggt agttagaatc ggtcgccagc tgatcgatcg
     1321 ggaaaccgcg gtgcagcaaa ataccttcat caccatcaat aaaagtaatt ttagattcgc
     1381 aggatgcggt tgaagtgaag cctgggtcaa aggtgaatac accttttgaa ccgagagtac
     1441 ggatatcaat aacatcttga cccagcgtgc ctttcagcac atccagttca acagctgtat
     1501 ccccgttgag ggtgagtttt gcttttgtat cagccattta aggtctcctt agcgccttat
     1561 tgcgtaagac tgccggaact taaatttgcc ttcgcacatc aacctggctt tacccgtttg
     1621 ttatttggct cgccgctctg tgaaagaggg gaaaacctgg gtacagagct ctgggcgctt
     1681 gcaggtaaag gatccattga tgacgaataa atggcgaatc aagtacttag caatccgaat
     1741 tattaaactt gtctaccact aataactgtc ccgaatgaat tggtcaatac ttccacactg
     1801 ttacataagt taatcttagg tgaaataccg acttcataac ttttacgcat tatatgcttt
     1861 tcctggtaat gtttgtaaca actttgttga atgattgtca aattagatga ttaaaaatta
     1921 aataaatgtt gttatcgtga cctggatcac tgttcaggat aaaacccgac aaactatatg
     1981 taggttaatt gtaatgattt tgtgaacggc ctatactgcc gccaggtctc cggaacaccc
     2041 tgcaatcccg agccacccag cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt
     2101 gcatgacgcg cagttataga aaggacgctg tctgacccgc aagcagaccg gaggaaggaa
     2161 atcccgacgt ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc cagggaataa
     2221 taagaacagc atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa
     2281 tctggaccta cagaccatcc ggttccccat cacggcgata gcgtccattc tccatcgcgt
     2341 ttccggtgtg atcacctttg ttgcagtggg catcctgctg tggcttctgg gtaccagcct
     2401 ctcttcccct gaaggtttcg agcaagcttc cgcgattatg ggcagcttct tcgtcaaatt
     2461 tatcatgtgg ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta ttcgccacat
     2521 gatgatggat tttggctatc tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat
     2581 atcctttgtt attactgtcg tgctttcact tctcgcagga gtcctcgtat ggtaagcaac
     2641 gcctccgcat taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc taccgctatc
     2701 gtcctgacgc tctacatcat ttatatggtc ggttttttcg ctaccagtgg cgagctgaca
     2761 tatgaagtct ggatcggttt cttcgcctct gcgttcacca aagtgttcac cctgctggcg
     2821 ctgttttcta tcttgatcca tgcctggatc ggcatgtggc aggtgttgac cgactacgtt
     2881 aaaccgctgg ccttgcgcct gatgctgcaa ctggtgattg tcgttgctct ggtggtttac
     2941 gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc agagaatttg
     3001 atgcagttgt aattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa atttcccaga
     3061 gcggtcagac ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat accgtttctg
     3121 cgcaaggcgg cattaccgtt gcgctgggta atacccatga agataactgg gaatggcata
     3181 tgtacgacac cgtaaaaggt tcggactata tcggtgacca ggacgcgatt gaatatatgt
     3241 gtaaaaccgg gccggaagcg attctggaac tggaacatat gggcctgccg ttctcgcgtc
     3301 ttgatgatgg tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg
     3361 agcaggcggc acgtactgcg gcggcagctg accgtaccgg tcacgcactg ttgcacaccc
     3421 tttatcaaca gaacctgaaa aaccacacca ccattttctc cgagtggtat gcgctggatc
     3481 tggtgaaaaa ccaggatggc gcggtggtcg gttgtaccgc actgtgcatc gaaactggtg
     3541 aagtggttta cttcaaagct cgcgctaccg tgctggcgac tggcggggca gggcgtattt
     3601 atcagtccac taccaacgcc cacattaaca ctggcgacgg cgtcggcatg gctatccgtg
     3661 caggcgtacc agtgcaggat atggaaatgt ggcagttcca cccgactggt attgccggtg
     3721 cgggcgtact ggtcaccgaa gggtgccgtg gtgaaggcgg ttatctgctg aacaaacatg
     3781 gcgaacgctt tatggagcgt tatgcaccga acgccaaaga cctggcgggc cgtgacgtgg
     3841 tggcgcgttc catcatgatt gaaatccgtg aaggtcgcgg ctgtgatggt ccgtgggggc
     3901 cacacgcgaa actgaaactt gaccatctgg gtaaagaagt tcttgaatcc cgtctgccgg
     3961 gtatccttga actctcccgc accttcgctc acgttgatcc ggtgaaagag ccgattccgg
     4021 ttatcccaac ctgtcactac atgatgggcg gtattccgac caaagtgacc ggtcaggcgc
     4081 tgacggtgaa cgagaaaggc gaagatgtgg ttgttccggg gctatttgcc gttggtgaaa
     4141 tcgcttgtgt atcggtacat ggcgctaacc gtctgggcgg caactcgctg ctggacctgg
     4201 tcgtatttgg tcgtgcggca ggtctgcatc tgcaagagtc tatcgccgag cagggcgcac
     4261 tgcgcgatgc cagcgcgtct gatgtagaag cgtctctgga tcgcctgaac cgctggaaca
     4321 ataaccgtaa cggtgaagat ccggtggcga tccgtaaagc actgcaagaa tgtatgcagc
     4381 ataacttctc ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag cagttgaaag
     4441 ttatccgcga gcgtctgaaa aatgcccgtc tggatgacac ttcaagtgag ttcaataccc
     4501 agcgcgttga gtgcctggaa ctggataacc tgatggaaac ggcgtatgca acggctgttt
     4561 ctgccaactt ccgtaccgaa agccgtggcg cacatagccg cttcgacttc ccggatcgtg
     4621 atgatgaaaa ctggctgtgc cactccctgt atctgcctga gtcggaatcc atgacgcgcc
     4681 gaagcgtcaa catggaaccg aaactgcgcc cggcattccc gccgaagatt cgtacttact
     4741 aatgcggaga caggaaaatg agactcgagt tttcaattta tcgctataac ccggatgttg
     4801 atgatgctcc gcgtatgcag gattacaccc tggaagcgga agaaggtcgt gacatgatgc
     4861 tgctggatgc gcttatccag ctgaaagaga aagatcccag cctgtcgttc cgtcgctcct
     4921 gccgtgaagg tgtgtgtggt tccgacggtc tgaacatgaa cggcaagaat ggtctggcct
     4981 gtattacccc gatttcggca ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc
     5041 caggtttacc ggtgatccgc gatttggtgg tagacatggg acaattctat gcgcaatatg
     5101 agaaaattaa gccttacctg ttgaataatg gacaaaatcc gccagctcgc gagcatttac
     5161 agatgccaga gcagcgcgaa aaactcgacg ggttgtatga atgtattctc tgcgcatgtt
     5221 gttcaacctc ttgtccgtct ttctggtgga atcccgataa gtttatcggc ccggcaggct
     5281 tgttagcggc atatcgtttc ctgatcgata gccgtgatac cgagactgac agccgcctcg
     5341 acggtttgag cgatgcattc agtgtattcc gctgtcacag catcatgaac tgcgtcagtg
     5401 tatgtccgaa ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg atgttgttgc
     5461 aacgtaatgc gtaaaccgta ggcctgagaa gacgcgcaag cgtcgcatca ggcaaccagt
     5521 gccggatgcg gcgtgaacgc cttatccggc ctacaagtca ttacccgtag gcctgataag
     5581 cgcagcgcat caggcgtaac aaagaaatgc aggaaatctt taaaaactgc ccctgacact
     5641 aagacagttt ttaaaggttc cttcgcgagc cactacgtag acaagagctc gcaagtgaac
     5701 cccggcacgc acatcactgt gcgtggtagt atccacggcg aagtaagcat aaaaaagatg
     5761 cttaagggat cacgatgcag aacagcgctt tgaaagcctg gttggactct tcttacctct
     5821 ctggcgcaaa ccagagctgg atagaacagc tctatgaaga cttcttaacc gatcctgact
     5881 cggttgacgc taactggcgt tcgacgttcc agcagttacc tggtacggga gtcaaaccgg
     5941 atcaattcca ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt
     6001 actcttcaac gatctccgac cctgacacca atgtgaagca ggttaaagtc ctgcagctca
     6061 ttaacgcata ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg ggactgtggc
     6121 agcaagataa agtggccgat ctggatccgt ctttccacga tctgaccgaa gcagacttcc
     6181 aggagacctt caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa ctcggcgagc
     6241 tgctggaagc cctcaagcaa acctactgcg gcccgattgg tgccgagtat atgcacatta
     6301 ccagcactga agaaaaacgc tggatccaac agcgtatcga gtctggtcgc gcgactttca
     6361 atagcgaaga gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc cttgaacgtt
     6421 acctcggcgc aaaattccct ggcgcaaaac gcttctcgct ggaaggtggt gacgcgttaa
     6481 tcccgatgct taaagagatg atccgccacg ctggcaacag cggcacccgc gaagtggttc
     6541 tcgggatggc gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt aaaaaaccgc
     6601 aagacttgtt cgacgagttt gccggtaaac ataaagaaca cctcggcacg ggcgacgtga
     6661 aataccacat gggcttctcg tctgacttcc agaccgatgg cggcctggtg cacctggcgc
     6721 tggcgtttaa cccgtctcac cttgagattg tcagcccggt cgttatcggt tctgttcgtg
     6781 cccgtctgga cagacttgat gagccgagca gcaacaaagt gctgccaatc accattcatg
     6841 gtgacgccgc agtgaccggg cagggcgtgg ttcaggaaac cctgaacatg tcgaaagcgc
     6901 gtggttatga agttggcggt acggtacgta tcgttatcaa caaccaggtt ggcttcacca
     6961 cctctaaccc gctggatgcc cgttcgacgc cgtactgtac tgatatcggc aagatggtac
     7021 aggcaccgat tttccacgtt aatgcggatg atccggaagc cgttgccttt gtgacccgtc
     7081 tggcgctcga tttccgtaac acctttaaac gtgatgtctt catcgatctg gtgtgctacc
     7141 gccgtcacgg ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg atgtatcaga
     7201 aaatcaaaaa acatccgacg ccgcgcaaaa tctatgctga caagctggag caggaaaaag
     7261 tcgcgacgct ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg ctggatgctg
     7321 gcgattgcgt tgtagcagag tggcgtccga tgaacatgca ctctttcacc tggtcgccgt
     7381 acctcaacca cgaatgggac gaagagtacc cgaacaaagt tgagatgaag cgcctgcagg
     7441 aactggctaa acgcatcagc acggtgccgg aagcagttga aatgcagtct cgcgttgcca
     7501 agatttatgg cgatcgccag gcaatggcag ccggtgagaa actgttcgac tggggcggtg
     7561 cggaaaacct cgcttacgcc acgctggttg atgaaggcat tccggttcgc ctgtcgggtg
     7621 aagactccgg tcgcggtacc ttcttccacc gccacgcggt gatccacaac cagtctaacg
     7681 gttccactta cacgccgctg caacacatcc ataacggtca gggcgcgttc cgtgtctggg
     7741 actccgtact gtcagaagaa gccgtgctgg cgtttgaata cggttatgcc accgcagaac
     7801 cacgcactct gaccatctgg gaagcgcagt tcggtgactt cgccaacggt gcgcaggtgg
     7861 ttatcgacca gttcatctcc tctggcgaac agaaatgggg ccggatgtgt ggcctggtga
     7921 tgttgctgcc gcacggttac gaagggcagg ggccggagca ctcctccgcg cgtctggaac
     7981 gttatctgca actttgcgct gagcaaaaca tgcaggtttg cgtaccgtct accccggcac
     8041 aggtttacca catgctgcgt cgtcaggcgt tgcgcgggat gcgtcgtcca ctggtcgtga
     8101 tgtcgccgaa atccctgctg cgtcatccgc tggcggtatc cagcctcgaa gaactggcga
     8161 acggcacctt cctgccagcc atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc
     8221 gcgtagtgat gtgttctggt aaggtttatt acgacctgct ggaacaacgt cgtaagaaca
     8281 atcaacacga tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg cataaagcga
     8341 tgcaggaagt gttgcagcag tttgctcacg tcaaggattt tgtctggtgc caggaagagc
     8401 cgctcaacca gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg attccgtttg
     8461 gggcttctct gcgttatgca ggccgcccgg cctccgcctc tccggcggta gggtatatgt
     8521 ccgttcacca gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc gaataaataa
     8581 aggatacaca atgagtagcg tagatattct ggtccctgac ctgcctgaat ccgtagccga
     8641 tgccaccgtc gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg atgaagtgct
     8701 ggtagaaatc gaaactgaca aagtggtact ggaagtaccg gcatcagcag acggcattct
     8761 ggatgcggtt ctggaagatg aaggtacaac ggtaacgtct cgtcagatcc ttggtcgcct
     8821 gcgtgaaggc aacagcgcgg gtaaagaaac cagcgccaaa tctgaagaga aagcgtccac
     8881 tccggcgcaa cgccagcagg cgtctctgga agagcaaaac aacgatgcgt taagcccggc
     8941 gatccgtcgc ctgctggctg aacataacct cgacgccagc gccattaaag gcaccggtgt
     9001 gggtggtcgt ctgacccgtg aagatgtgga aaaacatctg gcgaaagccc cggtgaaaga
     9061 gtctgctccg gcagcgcctg ctccggcggc gcaaccggcc ctggctgcac gtagcgaaaa
     9121 acgtgtgccg atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa
     9181 ctccaccgcc atgctgacca cgttcaacga agtcaacatg aagccgatta tggatctgcg
     9241 taagcagtac ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct ttatgtcctt
     9301 ctacgtgaaa gcggtggttg aagccctgaa acgttacccg gaagtgaatg cgtctatcga
     9361 cggtgatgat gtggtctacc acaactattt cgacgtcagt atggcggttt ctacgccgcg
     9421 cggcctggtg acgccggttc tgcgtgatgt cgataccctc ggcatggcag acatcgagaa
     9481 gaaaatcaaa gagctggctg ttaaaggccg tgacggcaag ctgacggttg aagatctgac
     9541 tggtggtaac ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt caacgcctat
     9601 catcaaccca ccgcagagcg caattctggg tatgcacgct atcaaagatc gtccgatggc
     9661 ggtgaatggt caggttgaga tcctgccgat gatgtacctg gcgctgtcct acgatcaccg
     9721 tctgatcgat ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt tgctggaaga
     9781 tccgacgcgt ctgctgctgg acgtgtagta gtttaagctt cacctgcact gtagaccgac
     9841 gtggataggg tgtcggtcta cggtttaaaa gataacgatt actgaaggat ggacagaaca
     9901 catgaactta catgaatatc aggcaaaaca actttttgcc cgctatggct taccagcacc
     9961 ggtgggttat gcctgtacta ctccgcgcga agcagaagaa gccgcttcaa aaatcggtgc
    10021 cggtccgtgg gtagtgaaat gtcaggttca cgctggtggc cgcggtaaag cgggcggtgt
    10081 gaaagttgta aacagcaaag aagacatccg tgcttttgca gaaaactggc tgggcaagcg
    10141 tctggtaacg tatcaaacag atgccaatgg ccaaccggtt aaccagattc tggttgaagc
    10201 agcgaccgat atcgctaaag agctgtatct cggtgccgtt gttgaccgta gttcccgtcg
    10261 tgtggtcttt atggcctcca ccgaaggcgg cgtggaaatc gaaaaagtgg cggaagaaac
    10321 tccgcacctg atccacaaag ttgcgcttga tccgctgact ggcccgatgc cgtatcaggg
    10381 acgcgagctg gcgttcaaac tgggtctgga aggtaaactg gttcagcagt tcaccaaaat
    10441 cttcatgggc ctggcgacca ttttcctgga gcgcgacctg gcgctgatcg aaatcaaccc
    10501 gctggtcatt accaaacagg gcgatctgat ttgcctcgac ggcaaactgg gcgctgatgg
    10561 caacgcactg ttccgccagc ctgatctgcg cgaaatgcgt gaccagtcgc aggaagatcc
    10621 gcgtgaagca caggctgcac agtgggaact gaactacgtt gcgctggacg gtaacatcgg
    10681 ttgtatggtt aacggcgcag gcctggcgat gggcaccatg gacatcgtta aactgcacgg
    10741 cggcgaacca gctaacttcc tggacgttgg cggcggcgca accaaagaac gtgtaaccga
    10801 agcgttcaaa atcatcctct ctgacgacaa agtgaaagcc gttctggtta acatcttcgg
    10861 cggtatcgtt cgttgcgacc tgatcgctga cggtatcatc ggcgcggtag cggaagtggg
    10921 tgttaacgta ccggtcgtgg tacgtctgga aggtaacaac gccgaactcg gcgcgaagaa
    10981 actggctgac agcggcctga atattattgc agcaaaaggt ctgacggatg cagctcagca
    11041 ggttgttgcc gcagtggagg ggaaataatg tccattttaa ttgataaaaa caccaaggtt
    11101 atctgccagg gctttaccgg tagccagggg actttccact ctgaacaagc cattgcatac
    11161 ggcactaaaa tggttggcgg cgtaacccca ggtaaaggcg gcaccaccca cctcggcctg
    11221 ccggtgttca acaccgtgcg tgaagctgtt gctgctactg gcgctaccgc ttctgttatc
    11281 tacgtaccag caccgttctg caaagactcc attctggaag ctatcgatgc aggcatcaaa
    11341 ctgattatca ccatcactga aggcatcccg acgctggata tgctgaccgt gaaagtgaag
    11401 ctggatgaag caggcgttcg tatgatcggc ccgaactgcc caggcgttat cactccgggt
    11461 gaatgcaaaa tcggtatcca gcctggtcac attcacaaac cgggtaaagt gggtatcgtt
    11521 tcccgttccg gtacgctgac ctatgaagcg gttaaacaga ccacggatta cggtttcggt
    11581 cagtcgacct gtgttggtat cggcggcgat ccgatccctg gctctaactt tatcgacatc
    11641 ctcgaaatgt tcgaaaaaga tccgcagacc gaagcgatcg tgatgatcgg tgagatcggc
    11701 ggtagcgctg aagaagaagc agcggcgtac atcaaagagc acgttaccaa gccagttgta
    11761 ggttacatcg ctggtgtgac tgcgccgaaa ggcaaacgta tgggccacgc gggtgcgatc
    11821 attgccggtg ggaaagggac tgcggatgag aaattcgctg ctctggaagc cgcaggcgtg
    11881 aaaaccgttc gcagcctggc ggatatcggt gaagcactga aaactgttct gaaataaata
    11941 tctgtaataa gaaatagccc tcgccgcttc cttctacagg aatggcgaag ggctgtcggt
    12001 ttcgacatgg ttggccatcg tatgatggcc ttttttatgc ttatcgcgat gattttcgct
    12061 gcgctatcag ggtaaattta tagtcatcgg tattaaaagc gttgcggcta tattcaaaca
    12121 cccggccatc aactaaatat ccacgcgata ctttttcaag aatcggctta gtctggctga
    12181 tattaagcag acggctcatt tcttcggttg gcatcagagg aatgatttcc tgttcgctac
    12241 gatcgataac cattttcttc acttcttcaa taaagtgata tttcgaattt tccatgacct
    12301 gccaggtgag atccgggaac aacgcaagcg gcatccaggt ttcttccagc gccattggct
    12361 tttgcttgcg atagcgcacg cgcttcacat gccacacacg atcctgcgcg gtgatttgta
    12421 gctgttgctg aagaaaatcg tcagccggaa tcacttcgaa tatcagaact tcactgtgtg
    12481 tatcgacgtg acggtccgtc agtttttcat caaaactggt taactgaaaa atatcgtaat
    12541 tgacccgctc ttcttttacg taagtcccgc tgccctgaat actttcgagg atctgctgtt
    12601 caactaactg gcgcaaagcc tgacgcaccg taacccggct gacgccaaac tctgtttgta
    12661 gcgctgattc agtgggtaac gcatcgccag gtttaagctc gccacgcgca atttgctcac
    12721 gaatgcgatc agcaatctgt cggtataagg gcttccgtcc catgatttag catctcatta
    12781 atacgaattt agccattatg cccgataaat tcatcctgta aataatacaa atacaataca
    12841 aataatttca atcaagtgaa attgatcaca taatggtatt gttttatcgg gcacactggc
    12901 gcgactataa aaacgatcaa gtgaggatca tgatgaacct gacgactctg acccaccgcg
    12961 atgcgttgtg tctgaatgcg cgctttacca gccgtgaaga ggccatccac gcgttgactc
    13021 aacgtcttgc tgctctgggg aaaatttcca gtactgagca atttctgaaa gaagtgtatc
    13081 gccgtgaaag ccttggcccg accgccttag gtgaagggct ggctgtgccg catggcaaaa
    13141 ctgctgcggt aaaagaagcg gcgtttgcgg tcgccacact cagcgagccg cttcagtggg
    13201 aaggcgtgga tggcccggaa gcggttgatt tagtggtgtt gctggcgatt ccccccaatg
    13261 aagcgggtac tacgcacatg caactgctga cagcgctgac cacgcgcctg gcggatgatg
    13321 agatacgggc gcgtatacag tcggcgacga cgcctgatga attgctctcg gcgctggatg
    13381 acaagggagg cacgcaaccc tcagcttctt tttccaacgc gccaactatc gtctgcgtaa
    13441 cggcctgccc ggcgggtatt gctcacacct atatggctgc ggagtatctg gaaaaagccg
    13501 gacgcaaact cggcgtgaat gtctacgttg aaaagcaagg tgctaacggc attgaagggc
    13561 gtttaacggc agagcaactc aatagtgcaa ccgcctgtat ttttgcggct gaagtcgcca
    13621 tcaaggagag tgagcgtttt aacggcattc ccgcgctttc tgtgcctgtt gccgagccga
    13681 ttcgccatgc agaagcgttg atccaacaag cgcttaacct caagcgtagc gatgagacgc
    13741 gtaccgtaca gcaagatacg caaccggtga aaagcctcaa aacggagcta aaacaggcac
    13801 tgttgagcgg gatctctttt gcagtgccgt tgattgtcgc ggggggcacg gtgctggcgg
    13861 ttgcggtatt actgtcgcaa atcttcgggc tacaagattt gtttaatgaa gaaaactcct
    13921 ggctgtggat gtaccgcaaa ctgggcggag ggctgctcgg gattttgatg gtaccggtgc
    13981 tggcagccta caccgcctat tcactggctg ataaaccggc gttagcgcca ggctttgcgg
    14041 ctggacttgc cgccaacatg atcggttccg ggtttctcgg tgctgtcgtt ggcggattga
    14101 tagccggtta tctgatgcgc tgggtgaaaa atcacttgcg tcttagcagt aaattcaatg
    14161 gattcctgac tttttatctc tacccggtgc tcggtacgtt gggagcgggc agtctgatgc
    14221 tgtttgtggt gggggaacct gtcgcctgga tcaataactc gcttaccgcc tggctgaacg
    14281 gtctgtcagg aagtaacgcg ctgttgctgg gtgccattct cggttttatg tgttcctttg
    14341 accttggagg gccagtgaac aaagccgctt atgcattctg cctgggcgca atggcgaacg
    14401 gcgtttacgg cccgtatgcc attttcgcct ccgtcaaaat ggtttcggca tttaccgtaa
    14461 ccgcttccac gatgctcgca ccgcgcctgt ttaaagagtt tgaaattgag accgggaaat
    14521 ccacctggct gttagggctg gcaggtatta ccgaaggggc gatcccgatg gcgattgaag
    14581 atccgctgcg ggttattggt tcgtttgtgc tgggctctat ggtaacgggc gctatcgtcg
    14641 gcgcgatgaa tatcggcctt tcgacacccg gtgccggcat tttctcgctc tttttacttc
    14701 atgataatgg cgcgggcggt gttatggcgg caattggctg gtttggtgcg gcattggtgg
    14761 gggctgcaat ctcgactgca attcttctga tctggcggcg tcacgcggtt aagcatggca
    14821 actatctgac tgatggcgta atgccataaa caaaaaggaa acgacgatga aagcggtatc
    14881 tcgcgttcac atcaccccgc atatgcactg ggatcgggag tggtatttca ccaccgaaga
    14941 gtcacgcatt ctgctggtca ataatatgga agagatcctg tgccgactgg aacaggacaa
    15001 cgaatacaaa tattacgtac tcgacgggca aacggcgatc ctcgaagatt atttcgcggt
    15061 gaaaccggaa aacaaagacc gtgtgaagaa acaggtagaa gcaggcaagt tgattatcgg
    15121 cccctggtat acccagaccg ataccacgat tgtttctgcg gaatccatcg tccgtaatct
    15181 gatgtacgga atgcgtgact gcctcacgtt tggcgagccg atgaaaatag gttatttacc
    15241 agattccttt ggcatgtccg ggcaactgcc gcatatttac aatggatttg gcattacccg
    15301 caccatgttc tggcgcggat gttctgagcg ccacggtact gataaaaccg agtttttgtg
    15361 gcaaagcagt gacggtagcg aagtgacggc gcaggtgctg ccgctgggct acgccatcgg
    15421 taagtactta cctgccgacg aagacggatt acgcaaacgc ctcgacagtt attttgatgt
    15481 gctggaaaaa gcgtcagtaa ccaaagagat tttgctgccg aatggtcatg accagatgcc
    15541 attgcagcaa aatatcttcg aagtgatgga taagctacgt gagatctacc ctcaacgtaa
    15601 gtttgtgatg agccgctttg aagaggtatt tgagaagatc gaagcgcagc gagataatct
    15661 ggcaaccctg aaaggggaat ttattgatgg caaatatatg cgcgtgcatc gcaccatcgg
    15721 ttctacgcgt atggatatca aaattgccca cgcgcgtatt gaaaataaga ttgttaatct
    15781 gctggaaccg ctggcaacgc tggcctggac gttgggtttt gaataccacc acggcttgct
    15841 ggagaaattg tggaaagaaa ttttaaaaaa ccacgcccat gacagcatcg gctgctgctg
    15901 tagtgacaaa gttcatcgcg agattgtcgc ccgtttcgaa ctggcagaag acatggcgga
    15961 taatctgctg cgtttctata tgcgtaaaat tgccgataac atgccacaga gtgatgccga
    16021 caaacttgtg ctgtttaacc tgatgccctg gccgcgggaa gaagtgatca acaccactgt
    16081 gcggctgcgc gccagccagt ttaatttgcg ggacgatcgc ggtcagcctg taccgtattt
    16141 tattcgccat gcccgtgaga tcgatcctgg cctaatcgat cggcaaatcg ttcattacgg
    16201 taattatgat ccctttatgg agtttgatat tcagatcaac cagattgtcc cttctatggg
    16261 ctatcgcacg ctttatatcg aagcgaatca gcctggcaac gtgattgcgg caaaaagtga
    16321 cgctgaaggg atactggaaa atgctttctg gcaaattgcg ctcaatgagg atggaacgct
    16381 acaactggta gataaagaca gcggtgtgcg ctatgaccgg gtgttgcaaa ttgaagaaag
    16441 ctctgatgat ggtgatgaat atgactattc acccgcaaaa gaagagtggg taattacttc
    16501 ggcgaacgcg aaaccgcaat gcgagattac tcatgaggcc tggcagagta gggctgttat
    16561 ccgctatgac atggcggtgc cgcgtaatct gcatgaacgc agcgcaagac aatccactgg
    16621 cagagtaggg gtggagttgg ctgtcactct gagtgataac agcaggcgca ttgatgtgga
    16681 tatcaacctt gataaccagg ctgacgatca tcgccttcgc gtcctggtcc ctacaccttt
    16741 taacaccgac agtgttctgg ccgataccca gtttggttcg ctaacgcgcc ccgtgaacga
    16801 cagtgcaatg aacaactggc agcaagaagg ctggaaagaa gcgccggttc cggtatggaa
    16861 tatgctcaac tatgctgcct tacaggaagg gcgtaacggt ctggctgtct ttagcgaagg
    16921 gctacgtgaa tatgaagtca tcggtgaaga gaagaaaacc tttgccatta cgttgctgcg
    16981 tggcgtgggc ttactgggga aagaagatct gcttttaagg cctggacgtc cttcaggaat
    17041 aaaaatgccg gtgcccgatt cacaattacg tggctccttt agctgccgtc tgagcttatt
    17101 tagttataca ggtacgcctt cgaccgccgg tgtagcccag caggcgcgcg catggctgac
    17161 gccagtacag tgttacaaca aaatcccgtg ggatgcgatg aagttaaata aagccggatt
    17221 caatgtgccg gaaagctata gcttgctgaa aatgccccct gtgggatgcc tgattagcgc
    17281 tcttaaaaaa gcggaagacc ggcaagaagt ggtattgcga ctgtttaatc cggcagaatc
    17341 gacaacctgt gaggcaactg tcgcgttcag tcgtgaggtg atttcatgta cgcaaacgat
    17401 gatggatgaa agcatcagca cagcggaaag agataattta aaggaactgg gggcattttt
    17461 accggggcag tcacggacat ttagctaccg ccttggctga ttattgcgct aaaagtgtat
    17521 ctgagggggg caataatttc aggccatctt cggatggcct gagattgttg ataaacaagt
    17581 ttgttagctt acgcagatac cagatttgac ctgcgtgaga accgcaggcc atttatcatt
    17641 tcagttaccc cttaaacgtt aaccacggtc gaaatctgac cgctggctga attaatccgg
    17701 cttcttgttg ggcttcgata accggaccaa tatctttata agccgctggc gcttcttcaa
    17761 aacgtcgttc ttcgcgcaat gtgacgcatt gccacggcag cgttgactct tcagtcgcgc
    17821 gtgagcgcat ggcctgccga cgctggctgc gtcctgctcc gtgactgcat gaccacagcc
    17881 actccgggtt gccgcaacct accgcaagcc acgaataatc gcccattgaa ccggggatta
    17941 acgccagatt gcccactcgt gccggtgttg cgcctttgcg atgaatattc atttcatgct
    18001 cgcgaaggat gatgttatgt ggcacatcca ccaccagatg cgaattgtcc tgatgaaaga
    18061 gattggcaag acatacgcgc accatttcgg caattgtgac gcgatttaac catgcgtaac
    18121 gcgccgccat tcctatcgct tgtagatatt cctccgccag tccgcctgcc agaccatata
    18181 acccgctttg tggatgttta tgtccggtgg gccattcagc acgggcgcga tccatccagt
    18241 gtcttccgac gtaaaagcca acatctcgcg atccactgtg gatcatcacc acaatatcgc
    18301 ctgttttcag cccttgttgc caggctacat ggcgatcaac cacgctatca acgacctgaa
    18361 gttcaataaa atgattacct gaacccggcg tacccagaca aggatcgcgg agtaatgcgc
    18421 ggtccgttgc ctgcagggcg ggtggggcat aacgcgaatg actgttatgc tcgttaagcc
    18481 caatgcagcg gtcaatctct tgtaccattc gggggatatt cgtgcgtatc cagagacctt
    18541 ctgatggcaa ggtcgacacg caatctgccg gtccgccatc aaaaagcgcg gtgaacgcct
    18601 gtgcggataa cggaacatcg cgctcatttt gcagtagcac ttgcgttaac cgctgagcga
    18661 tgacgtcttt atgcgcttcg gcttgctcaa gtgttaatcc ggtcgtcagc agccgcatgc
    18721 cacaagaaat atcggtgccg atggcggcgg gaatgactaa atccggcgtt gaggccacca
    18781 ccgcgccaac ggggacgcca ttgccgggat gaaaatctgg cgtcgcgcac gtctggcgca
    18841 cataacctgg gtggtctggt agcttcactt ctgcaaaatc aagcagttgt gttactgctt
    18901 tttcttccag cggcagttcg tccggcagca gtacgcgggc actggcacct gatggacgag
    18961 ataatgaaac aatattgttg gcgcgggaaa gttggatccc tttgcgcaga aaggctttat
    19021 caagccttga aagatgatgc ataacgctgt cctgaaatat agacgttaaa caaccccctg
    19081 tatggcgagg gtaattttgt ctttccggtc agcagccagc aaaagggaaa gaaaacgcgc
    19141 gtagtgtatg tggcgatagc aagtgttaca agtggtaaat ctggcttatt gttaaagagg
    19201 aattatcttt tgtgaagtct tttaaggcca tgactcattc aatttaaccg gacatagata
    19261 atatataaca tagggtatct aaatttatca ttgtatactt gtcataattc aagttgcatg
    19321 tgctgcatcg atgttcgctc acccaggtca cttacttatg taagcttctg gaaattcatt
    19381 atttgttccc ttcctgcaac ttgacttatt tagagtatat tcatgtatac acatcgagat
    19441 ttcagccagc agacgcgcat ctggcgtgcc gtggcggatc tgtagcggtt tttgcagagt
    19501 ttcggtgagg cgacggcaag ctgcaacgcc gtggttaacg accggtaaac cgttcagttt
    19561 agaggttccg gcttcgatgg attttttaat ccctacagcc gcttcttcgt accgattcag
    19621 acgggtatat gcatcgatgg tgctcggcag cagatcgcac tcttcttgta gtgttttcag
    19681 tagttcgatg tgttcgtcca tcagcgccac acctgcgcgc ggttggctaa gggttttacc
    19741 ttccttgtct gctttcaaca gcgcaagaga gaagcgttta tgctcaggaa tggtttgttg
    19801 gtatttaacg ccatcttcaa agttttcaac atccttgccg gtttcccagg ttttcagtac
    19861 ctgttgccgc tcggtcatga attcgtcatg ggttaatttc ttatttcgaa gttccattga
    19921 ttgcagcctt aattgttata gggttaagca ttgaatactg gtgcgcgcgg cggccagcgg
    19981 atcgactctg gcgacgtttg ccagcagtgg taatagcccc cgggaatcgc ggtaatattc
    20041 aaattcggtc ggtaagagaa tgcgtttacc gtcgtcgttt aactcgcggt atttcaacca
    20101 gtgatgcata tcgaactgac tggcacgcga gagccatccg ccggatccga cgactttacg
    20161 cacggttgtc aggtcacgtc ccatctgtaa atcgacattg ccgacgcagg tgcagacctc
    20221 ttttttggtg cccgcatgtc tttcggtggc ataacccaca cacagtccgg ccaggacggt
    20281 atcgaaatat ttttcttctg cattcgcggg taagtagtcg ggatgagcga ctagatgacg
    20341 gaggtaatga taaaaagcgt ccagctgtgc gggttgctga gcaaaattca cattgactaa
    20401 ctctttcgca ctttcaccaa ccaccattgc cgagacgcgc attcccagat ctccttccac
    20461 cgtccgtttt acgaagggtt ccggtacgcc atgtaaaaca gtgtcgggcg agagcgtgtt
    20521 ggcactggcg gaatagacat cggtggttgc gccacccatg tcgatcagca tgaactcttt
    20581 ccagctgtga tcgacattac tgattgcctt aaccagttca aagactgtcc acggtgtcgg
    20641 cattggttct tcgcctgttt tatcaacaac gacatccagc cctttccctt taacgatgcg
    20701 tttaaggaat atgtcgcaga tagcctggcg tgcagctaaa gggttcggat ggtcaagatc
    20761 gggcagaacg ttgtcgacga tggtcaggtc tttatgcccg aggatctcct gcacctcgtc
    20821 ctgaatatcc cggtttccgg cataaataat ggcgcagtcg agtttggatt ctgccaacac
    20881 acgggcgtta ttcagtccgt agctttcttc tccgccatcg gtgccgccgg tgaacaagag
    20941 aatatcgggc tgcgtttctt ccagtgcctg gatatcacgg cggttcagct tgtaggcgta
    21001 agattgcgca attttagcac ccgcagagtg agcggttact ttcgcggttt ccagggtgat
    21061 agaaggcacc aatcccattg cggcaaccgc cagcccacct ttggcagagg aggaatactt
    21121 caatgcaacc tcaccactgt ttaacagggg gagggcatca tctacgttta acacctggtc
    21181 caggctggag aaaaacccct tcgccagatg atgggtggtg gttggggtca gtacgtggtt
    21241 aaccaacgtt aacgcgtccc cctcccgggc gaagagggct gctttggtcc acgtcgatcc
    21301 aatatcgaca gagacgattt gcatcagatt gcctcttcaa gaatacgagt atcaacgtca
    21361 tgacgttggt tgatatcgtg ggccatcagc tgacaaacat cttctaagtc atgacttggg
    21421 gcaaagacgc gatcaaatcc catctctttg aatttggttt ccacgtcagc gaagtcatgt
    21481 tttccgacca ccaggttacc gcccacatag agcaggatgt cccccagtcc acgctcgatg
    21541 cagcgttcgc gcatgcccag acagtcgata tcgccatgcc catagataga ggagacgaca
    21601 atcgcgtcag caccagtttc aatggcagca tcaatatatt cgtcctggct gaccattacg
    21661 cccagattaa taactctgaa gtcatggttg ctaaaaacgc ggtccagaac tttattgcct
    21721 actgcatggc agtccgcgcc aataacgcca atcacaagtg ttgctttctt catgggtaat
    21781 cctttgtaag acagagagta aaataagtta cacacgtcgt atttatggcc gctcataacg
    21841 gcacaggttt aatatcacag gagtgtcttt atctgaccgt aatgttgcaa ttgatctatt
    21901 ttcaaaatgt ctttcgggaa atgattgttt aaagtgtgtt ttcaaattct c
//
LOCUS       DAZKJR010000085        21729 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKJR010000085 DAZKJR010000000
VERSION     DAZKJR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.085"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..453)
                     /locus_tag="ACHOWS_003229"
     CDS             complement(<1..453)
                     /locus_tag="ACHOWS_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFQ0048761.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDE"
     gene            complement(656..1057)
                     /gene="nikR"
                     /locus_tag="ACHOWS_003230"
     CDS             complement(656..1057)
                     /gene="nikR"
                     /locus_tag="ACHOWS_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFQ0048762.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1062..1868)
                     /gene="nikE"
                     /locus_tag="ACHOWS_003231"
     CDS             complement(1062..1868)
                     /gene="nikE"
                     /locus_tag="ACHOWS_003231"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFQ0048763.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1865..2629)
                     /gene="nikD"
                     /locus_tag="ACHOWS_003232"
     CDS             complement(1865..2629)
                     /gene="nikD"
                     /locus_tag="ACHOWS_003232"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFQ0048764.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENTARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2629..3462)
                     /gene="nikC"
                     /locus_tag="ACHOWS_003233"
     CDS             complement(2629..3462)
                     /gene="nikC"
                     /locus_tag="ACHOWS_003233"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFQ0048765.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3459..4403)
                     /gene="nikB"
                     /locus_tag="ACHOWS_003234"
     CDS             complement(3459..4403)
                     /gene="nikB"
                     /locus_tag="ACHOWS_003234"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFQ0048766.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4403..5977)
                     /gene="nikA"
                     /locus_tag="ACHOWS_003235"
     CDS             complement(4403..5977)
                     /gene="nikA"
                     /locus_tag="ACHOWS_003235"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0048767.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6088..6675)
                     /gene="acpT"
                     /locus_tag="ACHOWS_003236"
     CDS             complement(6088..6675)
                     /gene="acpT"
                     /locus_tag="ACHOWS_003236"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFQ0048768.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6730..7779)
                     /locus_tag="ACHOWS_003237"
     CDS             complement(6730..7779)
                     /locus_tag="ACHOWS_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0048769.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7911..9128
                     /locus_tag="ACHOWS_003238"
     CDS             7911..9128
                     /locus_tag="ACHOWS_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0048770.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9132..9689)
                     /gene="dcrB"
                     /locus_tag="ACHOWS_003239"
     CDS             complement(9132..9689)
                     /gene="dcrB"
                     /locus_tag="ACHOWS_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFQ0048771.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(9762..10427)
                     /locus_tag="ACHOWS_003240"
     CDS             complement(9762..10427)
                     /locus_tag="ACHOWS_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFQ0048772.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10649..10894
                     /gene="tusA"
                     /locus_tag="ACHOWS_003241"
     CDS             10649..10894
                     /gene="tusA"
                     /locus_tag="ACHOWS_003241"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFQ0048773.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKGG"
     gene            complement(10996..13194)
                     /gene="zntA"
                     /locus_tag="ACHOWS_003242"
     CDS             complement(10996..13194)
                     /gene="zntA"
                     /locus_tag="ACHOWS_003242"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFQ0048774.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVMLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13268..13894)
                     /locus_tag="ACHOWS_003243"
     CDS             complement(13268..13894)
                     /locus_tag="ACHOWS_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFQ0048775.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14035..14394
                     /locus_tag="ACHOWS_003244"
     CDS             14035..14394
                     /locus_tag="ACHOWS_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFQ0048776.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDI
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14397..14666)
                     /locus_tag="ACHOWS_003245"
     CDS             complement(14397..14666)
                     /locus_tag="ACHOWS_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFQ0048777.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14656..15252)
                     /gene="rsmD"
                     /locus_tag="ACHOWS_003246"
     CDS             complement(14656..15252)
                     /gene="rsmD"
                     /locus_tag="ACHOWS_003246"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFQ0048778.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15402..16898
                     /gene="ftsY"
                     /locus_tag="ACHOWS_003247"
     CDS             15402..16898
                     /gene="ftsY"
                     /locus_tag="ACHOWS_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0048779.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPELVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16901..17569
                     /gene="ftsE"
                     /locus_tag="ACHOWS_003248"
     CDS             16901..17569
                     /gene="ftsE"
                     /locus_tag="ACHOWS_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0048780.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17562..18620
                     /gene="ftsX"
                     /locus_tag="ACHOWS_003249"
     CDS             17562..18620
                     /gene="ftsX"
                     /locus_tag="ACHOWS_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0048781.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18865..19719
                     /gene="rpoH"
                     /locus_tag="ACHOWS_003250"
     CDS             18865..19719
                     /gene="rpoH"
                     /locus_tag="ACHOWS_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFQ0048782.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19991..21094
                     /gene="livJ"
                     /locus_tag="ACHOWS_003251"
     CDS             19991..21094
                     /gene="livJ"
                     /locus_tag="ACHOWS_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFQ0048783.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQVGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21282..21665)
                     /gene="panM"
                     /locus_tag="ACHOWS_003252"
     CDS             complement(21282..21665)
                     /gene="panM"
                     /locus_tag="ACHOWS_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFQ0048784.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5160 a         5731 c         6027 g         4811 t
ORIGIN      
        1 ttcatccagt ttcgccacgc cgccgcgcac cagccagagt gactcgctgc ggctgtaacc
       61 gtcctcaccg ggaaacaggt gctcaaaata caggcttctg ccgccgttat cactgagtat
      121 cagtgtgtta tcgcgcagct gtaagcggat atccgcaggc attttccagc cggggccgag
      181 gctccccacc ggcgcgggcg tttttgtccg gtaactgctg taggtgcggg agagaatgaa
      241 cggcagcggg ccgggcaggg caaggtcggt ttcgccggga aggaccttcg caccgagcag
      301 cggattgacc ggatgaccgg acgtcacccc gccggggcac accgaacagg ccacaccggt
      361 gggggcacca atacgtaccc cggcagaacc ctgaacaatg ctaccgccat actgcgtcat
      421 gtcgccctga cgcgccgccg gttttccgct catcgtaaaa tccttttcta ttattttaat
      481 gacagtatct tcatacgaaa aagcgaagaa aaacttgtgg tcacaatgaa ttgaagattt
      541 ttaggaatat atttagaacg ttacaaaaga gaaggttcgg atagggtatt tagagaggaa
      601 tcaggtgtgc tagtggccct ttgttgatcg ttcaccaaag gacccaataa taggcttaat
      661 cttccttcgg caagcactgc aaatgcccgt gccgcacgcc gcgctgggcg ataacgtcat
      721 cggcaaaatg ctgcacgtcg cccatatcgc ctttcagcac ggcgatttcc agacagtcgt
      781 cgtgattgat gtggacatgt agcgtcgcga cggagagatc gtggtgatgg tgctgggtgg
      841 agacaatacg gctggctaag tcgcgttttt cgtgttcata cacataggag agcaccgcga
      901 aaccttgcgt gccgtgctgc tgggtagctt cctgcgccag ggcgctacgc agaatatcgc
      961 ggatagcttc ggaacggttg ttatagccac gacgctggct caggctgtcc agcgtctcca
     1021 gtaaatcgtc atcaagcgtg atggtaactc gttgcataga cttaaacctt ttctgtggtg
     1081 cgacggcgca cggggaatgc gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag
     1141 gaaaaggtta atttatctcc caccacctgg gtttcgacga tttgtccgtt gtccatcacc
     1201 atcacccgct gacagaagcg ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg
     1261 gtgccaaact gttgttgcag ctttttcagc aggcgaatga cgcccgcctg taacacgaga
     1321 tcaaggttag aaacggcttc atccagaatc agtagtttcg gttcgaccgc cagcgcgcga
     1381 gccaggcaga cgcgctggag ctgaccgccg cttaactgcg gtgggcgttt gtctagaacg
     1441 ctgtcatcga gatcgaccgc cttcagcatt tcgctggcac gcgccagttg ttcggctttt
     1501 ttcagcgata gcaggtggcg catcggttca cgcaggatct cgcgcacggt ttttcgcgga
     1561 ttcacggcgc tgatggagtc ctgaaatacc atctgaatat cgcggcggaa cgctttacgc
     1621 tgggcgcgat tgagctttgc caacggttcg ccgcgccagc taatattccc ttgtgaggga
     1681 gactctaagc ctaccagcag ccgcgcgaga gtgctttttc cgcagccgct gcgccccagc
     1741 agagcgacgg tttcgccgct tttcagggtc agggaaacgt tattcagtac cgcctgatgc
     1801 tgatgttttc cgctaaatcc accgtgcgca tagtgatgtg aaaggtcgca gacgttaagt
     1861 aaagtcatga tgccagctcc ataccgtaga gggcgagatg agcggaaacc aggctgcgcg
     1921 tcaccgtatg tttgggggcg ttaaacagcg tttctacatc gccctgttcg acaattttac
     1981 cgtgtgacat caccgccacg tcatccgcca gacgcgccac aacgcccata tcatgggtga
     2041 ccagcagcat tcccggtgct tgtttttgca taatgctttc cagcagatcg aggatgcgcg
     2101 cctgtgctac cacgtcgagg tcggtggtcg gttcatcggc gatgataaac ggcgattcac
     2161 acaacaccgc catcgcaatc atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt
     2221 acagcttcaa cacgcgcgcg gtgttttcca accccaccgc ttctatggca gcggtaagcg
     2281 tagcgtcatc ggcgggtttc cccagcgcca ggcaggtttc acgcgcgtgg gtgtgcatgg
     2341 tgtgtagcgg attaaaggcg ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc
     2401 gcagggcaca gggagaaacc ggtttgccat cggctaaaat ttcccccgcc gtctggcgaa
     2461 cgcctgcggg cagaatgccc agcgtcgcgg cgcaggtcag tgatttcccg ctgccgctac
     2521 cgccgactaa cgccagcaca cgcccgcgtt taagggttaa cgatacgccg tgcaccagcg
     2581 gttgtgcggc ctgtagcgcg atattacgta gttcaatctg ttgcggcatt agtgtgcgtg
     2641 ctccgtcacc agatgaggat ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc
     2701 catcacgctg ataaacagcg ccagccccgg ccagaacatt tgcagcggct gggtccagat
     2761 atactgacgc gcgtcgttaa tcatcacgcc ccattcggcg gtcggcgcgg tcacgccgag
     2821 accgaggaag gacatccccg cgacgtgtag catcatatga ccgatatcca gcgtcgccag
     2881 caccagcagc gaggggatca ccgcacctgc cagatgatca ataaacaccc gcacatggcc
     2941 cgcgccagaa agccgtgatg ccagcacaaa ctcgcgctgg cgcagtgaaa tcaccaggct
     3001 acgcaccatg cgtgcatacc acgcccagtg cgacagggcg atggctataa tcacattggt
     3061 caacccggtg ccgagcacgc cgaccataaa gaacgacaga atcgaggttg ggaaggtcat
     3121 aaacatatca gcgacgcgca tggtggcctg atcaacgcgc ccgccaatca gcccggcgct
     3181 gccgccaata atcagcccta atgtcagcac caacagcagg caggccatta ccgagccgag
     3241 cgacacgcgg gtcgccgcca tcagccgcga gaaaatatcg cgccctaaat gatcggtacc
     3301 cagccagtgc tgcgcatccg gtgaaagcag gcgcgacggc agatcaatcg cctgggggtc
     3361 atacggcagc caccactggc tggtgagcgc aatcagcgcc agcagggcga taatgatcag
     3421 cgccaggcgt accgaccagc gggaagagag gaaaaagttc acgcgtgcgc tccttcatga
     3481 cgacgaatgc gcgggtccag cgcggcgttg agtaaatcaa caatcaaatt acagaccaca
     3541 aaaaccacca ccatcatcag cgtaaagcac tggatcaccg gatagtcacg gttaaaaatc
     3601 gccgacaccg catagcgccc gacgcccggc caggcaaaga tgttttcgat aatcatcgtt
     3661 ccgccaatca gttcgccaat gtgcatcccc acggcggtga tcatcggcag cgaggcattg
     3721 cgcagaatat ggcggcgttc ggtctgtttg tcgttcaggc cgcgtagccg agcccaggtg
     3781 acgtgacgct gaccggcgac gtccagcata ctggcgcgca gcagccgcgc gttaatcgcc
     3841 agcgacataa aggcaatgga aaccgcaggc aaaatgatgt gctgccagcc gccgtagccc
     3901 atcgcgggta gccattgcag atataccgaa aacgccatca ccagcaaaaa cgccagccag
     3961 aagttgggca tcgacacgcc gagaaacgcg atgaaacgta cggcgaaatc cggcagacga
     4021 tcgcgatggc gcgccgccca gataccgagc ggtacggaag tgagcagaat taacaccagc
     4081 gcggcacctg caagttccag cgtggcgggc aggaagttca gcatatcgtc cagcaccggg
     4141 cgttggctgg cgaatgagat accaaagtca agatgcagcg ccttccacaa ccaggtaccg
     4201 tactggacgt acagcggctg atccagcccc agcatggtgc gggtagaggc cagcatctcc
     4261 ggcgtcggcg gcaggttaga taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg
     4321 cgcagcatca gaaaaatgat caccgaggcg gcgagcacca tcgggatcag cagcagaaag
     4381 cggcgtaata cgtaacgcaa cattaaggtt tcaccggttt aatctgttcg aacggaattt
     4441 cggtggcgat cggcgcgtag gggatgttac ccagctccgg ttttgatacc accatcattg
     4501 agatgtaact gataggcaga taaaccgcct cgtcatgcag acgggtcaga atgtcgcgat
     4561 acagcgcctg acgttgcgtt tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa
     4621 tcagcggctt gtcggctaaa ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca
     4681 ttgaactcag gaaggcgtgt ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc
     4741 caaaacgacc gtcgcgctga cgagcataga tactgctctc ttcttcgcca atcagcgaga
     4801 catctgcgcc aatctggcgc atatcagcct gaatgatttc cgccatcgat ttgcttaacg
     4861 catcggtgcc gatgaacgac agttcaatgc gcagcggctg accatttttc tcgcggatgt
     4921 ctttgcctgc aggcagcgtc caaccggctt tttccagcaa cgctttcgct ttctgcggat
     4981 cgtactggcg cggtttcagg ccgaggttgg cgtagggcac agaaggggca aacagggtgt
     5041 cggcgacctg ctgggtacca tacaacgcgt tatcaatcag cgattttttg ttcaccgcgt
     5101 aattaagagc ttcacgtact gccagctcgt tggtgggggc tttggcggta ttgagcgcca
     5161 gcatcacggt ttcgatcggc tgtgacagtt gagtgtggta agccgggttc tggctaaagc
     5221 gggcgaaggt atcgagcggt aataaccctt cgtttccgta cagcaggtcg atatcgccag
     5281 tttcaaacgc caccgcacgg gtagtcgggt ccgggatcac gttaaaggtg atctttttaa
     5341 tcgctggctt ttcgccccag tagttttcgt tacggacgaa gacatcgtac tgattcagtt
     5401 tcgattcctg taaaacccac ggcccggtgc caatcggcgc tttaattccg ttcatggttt
     5461 cgtggttttt aaactgcgag ggtgcgataa aacggaaagg acggggcagg gccagttctt
     5521 gcaggaaagg atagtaggcg cttttcaggg tgatttgcag ctcgttttta ctgagtgctt
     5581 tgacatcaac aatctggttt gccagctcca gccaggcgtg acgttgacgg ttatcgagca
     5641 ctgcgcggaa gttttctgcc gccgcctctg catcaaacgg ttcgccgttg gagaatttca
     5701 cgtcatcacg cagggtgaag gtccaggttt taccatcttc tgaatgagtc cagctttttg
     5761 ccagccacgg gatcaccgaa ccgtctgcct gatatttcac caatggttca taaaccatgc
     5821 tctgggcgaa catctggtta ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg
     5881 gccaggcggt ggtgatttca tctggtgcag cggcatggac gataaaagac gcacaagcca
     5941 gcagcgcaaa tagagtgcgg cggagtgtgg agagcatggt aaccccaatg gattaaaata
     6001 gatggcggaa ataagtatga cgattttaag tattcgtcat actgattacc tgttctggat
     6061 caatagatgg gcagtcgggt gggcggatca gttaactgaa tcgatccatt gcacactgtc
     6121 ggcggtgagc gtaaaggggg taggggtgca gatcgccagg cttaaattgt ctagctgaca
     6181 atggctgact gacagtgatg agtgataggt gctgtctacg ctgacgattt gccaggcgct
     6241 gccgccacgc tgtttaacga tggcttcttt gcgcgtccag atgcgccaga acatttccag
     6301 ttgctgatca ggatgcacgg cgtccatctc ggcatgttcc ccaaggctga atacggcgtt
     6361 cgccagccag cgccagttgg cgcgcgggcg aatcacttcg atatcgcagc caacttcgcc
     6421 ttcatcgctc aacagcaggg cgatatcgtc accgctatgg cttaagttga accatagcgg
     6481 cgtttccggc gcgaatgccg gtttgccttg ttcgccatag atgatctccg gtagcgggga
     6541 aagcgtgtgc gaaagcaatg cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg
     6601 tgcttgctcg cgtaaagccg gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag
     6661 aactatccga tacatatcag ggccaacgtt taatggaaaa tgaaagtgcg tatcgtaaca
     6721 cttgtcgcct catcccggta accgactttt cggtctgcct ggccccagta aaatcgccag
     6781 tttgctaccg cctttggtgg tttccatcca gattttacac acgctggtta atggcaccga
     6841 aagtagcatc cctaccgggc cgagcagcca tccccaaatt aacaatgaaa gaaataccac
     6901 catggtggac atccccagac gatggcccat catccgtggt tctaaaatat tgccgatgac
     6961 catatggacc actaaaaaca atgcgccgac cagaatacat tcgtaaatac cattaaacag
     7021 cagcacctga atcattggcg gtacggcgga aattaccgca ccgatattgg gcacgtagtt
     7081 gagcaaaaac gccagtaccg cccacatcag cgcaaactgt acgcccataa gttccagccc
     7141 cagccagacg ataacgcctg tccataaact tagcagtgtt ttcaatgcca gataatgcga
     7201 aacgccttta agtgcgcggt gtagtccggc gatgtgaatt tgtggattat tcaacgcgaa
     7261 acgcattttg taagggacgt ggcgcacttc aaacagcata aaaactacgg tcatcaccag
     7321 caaaagcacg ctcgccattg ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac
     7381 cactttttcc gagtccatcc gctgcagcat tcgctccggt gacatatgca aattaagaaa
     7441 aggcaacatc tcctgcaatt taaaaagttt gcgcgtcagc tccttattaa acttcggcag
     7501 catagagata aattcgttaa acgatgccgc cagtacgccg accagcgcag ttagtgcgat
     7561 cagcatcacc accaccacaa tcgtaatggc aacggggcgt tgtactcccc gacgaataaa
     7621 ccaggtgacg agcgggttga ggacgatggc aaaaaacagc gccagtaaca gctgcacaat
     7681 gatatctgcc gctgcgtgaa tgcccgcgag gatcactacc agcgaggcca gcttgagcag
     7741 aatgtgcatg cccgttttat cgggttgagg ggtttccatt ggggcttcct tgtgactttt
     7801 tgtattaagt gtagcgggag tcacgccagc aatattctga ttcttactgc tgattttcac
     7861 gtcagcacgt ggtaaaaatg aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac
     7921 ccgtagccga acccgcgcta aacggattgc gcctgaattt gcgcattgtc tccattgtca
     7981 tgtttaactt cgccagctac ctcaccatcg ggttgccgct cgctgtatta ccgggctatg
     8041 tccatgatgt gatgggcttt agcgcttttt gggcaggatt ggttatcagc ctgcaatatt
     8101 tcgccacctt gctgagccgc cctcatgccg gacgttacgc cgatttgctg ggacccaaaa
     8161 agattgtcgt cttcggttta tgcggctgct ttttgagcgg tctgggatat ctgacggcag
     8221 gattaaccgc cagtctgccc gtcatcagcc tgttattact gtgcctgggg cgcgtcatcc
     8281 ttgggattgg gcaaagtttt gccggaacgg gatcgaccct gtggggtgtt ggcgtggttg
     8341 gctcgctgca tatcgggcgg gtgatttcgt ggaacggcat tgtcacttac ggggcgatgg
     8401 cgatgggtgc gccgttaggt gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt
     8461 taatcattat gggcgtggcg ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa
     8521 aagccagtaa aggcaaaccg ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg
     8581 gtatggcgct ggcactggct tccgccggat ttggcgttat cgccaccttt atcacgctgt
     8641 tttatgacgc taaaggttgg gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt
     8701 ttgtcggtac gcgtttgtta ttccctaacg gcattaaccg tatcggcggc ttaaacgtag
     8761 cgatgatttg ctttagcgtt gagataatcg gcctgctact ggttggcgtg gcgactatgc
     8821 cgtggatggc gaaaatcggc gtcttactgg cgggggcagg gttttcgctg gtgttcccgg
     8881 cattgggcgt agtggcggta aaagcggttc cacagcaaaa tcagggggcg gcgctggcaa
     8941 cttacaccgt atttatggat ttatcgcttg gcgtgaccgg accactggct gggctggtga
     9001 tgagttgggc gggcgtcccg gtgatttatc tggcggcggc gggactggtc gcaatcgcgt
     9061 tattactgac gtggcgatta aaaaaacggc ctccggaaca cgtccctgag gccgcctcat
     9121 catcttaaaa cttactgaat aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct
     9181 ttttgctgat tgtcagctgg cagcgtaatt tgcatggtca gcagttgatt acccacgtta
     9241 cccagaataa cggaagagta cgccgtctgg cctttcgcgg agataatgct gtctaactgc
     9301 tgcattttgt gacctttcag ctcaatggct ttattggtta ccacttgcag ctgcggatcg
     9361 cggctacgtt gctgatcttc cagacgcttc gccaacaccg ccagatcttc tttcggatca
     9421 tcgcccatga tgacgatgac tgctttttgc ccagtggcgt cggaccagac atgcatgtta
     9481 ttggcctgcg ttcccagctt accgctctgg tcggtcatat ccgctggcag cgagaaactt
     9541 aacttgccat caagcaggtt gacgggattc ccggtagcgt tactttccgc gaccgacccc
     9601 tgcgccgtag cgttagtgtc tttatcatca caggccgcaa gccccataac cagcaggcca
     9661 attccgacat atttaaccag attgcgcatt gacttcttcc tttcgataaa cggccataac
     9721 ggctcattca tccatcttat cacaactctg ataacgaacc tttaactcgc ctgcaaagcg
     9781 ttgatttcag atttatctgc cagtcttttc aacagcatat tgagtaatac gccatacatt
     9841 ggcaggaaga aaacgatact gattaacact ttgaaacagt aatcgaccag cgcgatttcc
     9901 atccagtgtt cagccataaa ggcatccggg ctacgccaga aggcaatgaa gaagaaggcc
     9961 agcgtgtcgc tgacgttacc gaacagtgtg gacgctgtcg gtgccagcca ccagtgacga
    10021 ctctgacgca ggcggttaaa aacgtgcacg tcgaggatct gccccagcgc gtaggccatg
    10081 aaactggcgg tggcgatacg ggcgacaaac aggttgaagt gggcgagcgc gccgaatccc
    10141 tgccaggaac ccatatagaa cagcgacgag atgacgtagg agatcagtaa cgccgggatc
    10201 atcaccgcaa agatgatacg acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg
    10261 gtagcaagga aaataaacgg aaagctaaat gcgccccagg tggtatggaa accaaaaatg
    10321 gagacgggaa gctgaaccag atagttactg gaggtgatca ccagcagatg aaataacgat
    10381 aaccagaaca acgccttata gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt
    10441 gattaaccat tggggtgagg gaacccaata cgtacgacac gctattttac cttgattaaa
    10501 taacgttctt accctttttt cgagccgccg catgatactg ctttacgttg acaatgcaat
    10561 ggttaatttt cacgcaatcg ttaacctggt ttgcttacgg actcacaggg cgtaaactag
    10621 cgccgttttt ttatgtgatg agaagaaaat gaccgatctc ttttccagcc ctgaccacac
    10681 actcgacgca cttggcctgc gctgcccgga accagtaatg atggtgcgca aaacagtgcg
    10741 caatatgcag ccaggcgaaa cgttgctgat tatcgccgac gatccggcca ctacccgcga
    10801 tattcctggg ttttgtacct ttatggaaca cgaactggtt gctaaagaga cggatgaact
    10861 gccttatcgt tatttgattc gcaaaggcgg ttgatgggga aagattggct tcgatgccgc
    10921 cttttcccct caccctaacc ctctccccag aggagcgagg ggaccgatcg cgctcaatgt
    10981 tgcgatcggt ttgccttatc tcctgcgcaa caatcttaac gcattcgctg tcaccagcac
    11041 cgtcgcaccc gtatctgcca gcaccgccag ccatagcccg gtcattccta acagtgtggt
    11101 gacgaggaag atccctttca gccccagcgc aatagtgatg ttctggcgga tattggcgtg
    11161 agtggcgcgt gccagttcaa tcatttgcac caggccgcgc aggtggttat gggttaatgc
    11221 tgcatcggcg gtttccagcg ccacgtctgt gccactgccc attgcaatcc cgatggcggc
    11281 ggctttcatc gctggcgcgt cgttaatacc gtcaccgacc atcgccagcg gcgcatgttg
    11341 attcagctcg gtcaccgctt tgactttatc ttccggcaac aggcccgctt taaactctag
    11401 ccccagttcc ccggcgattg ccgccgctgc gcgtggatta tcgccggtga ggatcacccc
    11461 tttgacgccc agcgcgttca gttcactgat ggcagttgca gcatcggcgc gcagggtatc
    11521 ctgtaacgca atgacaccaa gcacgtcatc gttacgtact accagcacta ccgtttgccc
    11581 ggcgctttcc agttcgttaa ttaaaccagc aaatgcatca gcgggatgtt tcccggcagc
    11641 gcatatcaac acgcgctcac cgttaacctg cgcttcaatg ccagacccaa ccagcgcccg
    11701 ctgtgattgg gcggcgggaa tggcgagttc agcaacctgt gcttctcgca cgatggcttg
    11761 cgccagtgga tgcgtcgcgc cttgctcgac cgccgctgcc agtgtcagca gttcggattc
    11821 actaataccc gttgccggat gaatcgcggt aacgcgcggt ttaccgacgg tcagcgtacc
    11881 ggttttatca aacgccacct gggtaacacg acccagttgt tccagcgccg ctccgccttt
    11941 aattaacgcc ccacgacgcg ctgctgctgc cagcccggag gtaatcgccg caggcgttga
    12001 gataactaac gcacacgggc agccaatcag cagcagcgtc agccctttat aaatccactc
    12061 ttgccagctg gcggcaaaca gcagtggtgg caccagcgtt accagcagag cgacggccat
    12121 aatcgctggc gtgtaaatac ggctgaaacg gtcgataaac cgctcaatgg gagcgcgacg
    12181 ctcttcggct tcttcgatca gtttcagaat ccggtcaatg gcgctggcac ccggttctga
    12241 cagcacttcc agcattacca gacggtctac gctggtggcg cctgcaggaa ctttatcgcc
    12301 cgtcgcgcgc tccacaggaa tggattctcc ggtcagggcg ctttcatcaa aactggcaaa
    12361 cggtgagagc agtttaccgt cggcaggcaa acgcccacct gccgcaactt caatcacatc
    12421 gccaggccgc aggctgttta ttgccacctc ttcacgctca ccgttacgca gacgcgtggc
    12481 ggtttctggc ttcagcgcca ttaacgcgct gaccccttga cgcgcgcgac tggcggccca
    12541 gccctccagt cgttcgccaa tcaaaaacag cagcaacacc atcgcagctt cagccgttgc
    12601 gccaataaac agcgcaccaa tagcggctac gctcattaac gtttcaatgg cgaagtagct
    12661 gccggatttg atcagccgta atgcctgacg agcaatcggg tacagcccaa ccagcgtggt
    12721 cgcgataaac gccagttgcc cgaacggatg attgaactgc tccagacccc agctgattgc
    12781 catcatcacg attagcgtaa tcagcggcag attctctttc aggcgtgatg cttgtggttc
    12841 gtcggcggac tgttcatcgc gcagggaata gcctgctttt tgcaccgcag attcaacctg
    12901 ggcacggatg tcattgtcgg catcgaccac cagtttttcg gtggcgaaca acacctgcac
    12961 ctgattcacg cctgcaagct ggcgcacggc attttctacc ttgcgcgcac aggcggcgca
    13021 gtccatgccg ctgactttcc agctatagcg ggtgccggag acgttttcag agagagttgg
    13081 cgtgctggaa catgcgccgt cgcagcaaca gtcgttggcg ttctgtaccg tggttagcgg
    13141 tttgaacgca gcaaattgag gggctttctt gccgtgattg tcaggagtcg acatggcatc
    13201 ctccggttaa gttttttctc attaaccgaa ggatacactc tggagtcgac tccagagtca
    13261 agttttatca gagatacagc gagcggacga tgaggaaatg accggcaaag tagcaggccg
    13321 cagcaatcgc gttatctgcc cggaagcggc ggcgatagtg gctacccagc cagacaaagt
    13381 tactgataaa cagcaacgaa gcgccgacaa acgcagagag cgccggagct gtcggacgga
    13441 agaaccacag ttcacctgcc agccacacca tcaccagcgt catgccgata aacgtgcaga
    13501 taggccaacg atactcttcc agacgcgtcc agataatcgc cagtaacagc gcacccagaa
    13561 ccagcaacac cagtggcaga ggccagaaga aagagagcgt catctgactg gcaaaataga
    13621 tggtgtacag caggtgcgag aggaaaaacg cgccgatggc gtacatcaga cgttgacgtg
    13681 gcaacagggt tagcgcatcg cccagcagtg aggcgcacag ccctgccagc accagatagc
    13741 taatggcgtc gaacatcggc gcttgccagg ccagtaacag caggagaaga agggttaacg
    13801 gtttaaacac ccagcgttgc caggttggcc cacgatacga tgcatccaca gatagccatg
    13861 cggaaagaca gacagcgata aacgaccaaa gcatcttagt tccttgaatt cgttattttt
    13921 tcgtaagcgg aatgcctacg gtcttgcttc cagtttagtg gcgagggtgg acggatgaca
    13981 acaccataac tggcagggta tgcttatttc cgcattttcc gatgagggat aaagatgagc
    14041 aaaccacctc ttttctttat tgttatcatt ggcttaattg tcgtcgccgc atcgtttcgt
    14101 tttatgcaac agcgacggga aaaagctgat aatgatatgg ctccgctcca gcaaaagctg
    14161 gtggtggtga gcaacaagcg ggaaaaaccg attaacgatc gccgttcgcg ccagcaggaa
    14221 gtgactccgg caggcaccag tatgcgctac gaggcaagct tcaaaccgca aagcggagga
    14281 atggagcaga cgtttcgcct cgacgctcag cagtaccatg ccctgacagt gggcgatata
    14341 ggtacgctga gctataaagg aacgcgcttt gttagctttg taggcgaaca ataaggttat
    14401 tttttaactt taaatttctt ctgccagacc agcagttcaa acacgccgaa aaggaaaatc
    14461 cgcacctttt cggcggtggt catctgcggg ccatctttag gtaaagtgga tttcaatagc
    14521 gccagctgca taccatgcat cagcacggta aaaatcagcg ccacgttaac gaaaatattc
    14581 agcgggcgcg ggaagggatg caccagattg aggattaaaa atccccaaac gcagagcatt
    14641 aacaaacgac caatattaat cagcatcact ttctccttgt gcttcgcgtt gatacagccg
    14701 ataagccacc tgacccgcca ctttttcccg atgtaatgac cagtttgccg gaacagtggg
    14761 cagaccgttt tcgacttcgc tttcgacata aatcagggct tcgtcagcca gccagccgtt
    14821 atcttccagt aaatttatcg tctcttctaa caagccacgg cggaacggtg gatcgacaaa
    14881 cacgatatta tgcggtgtgc ctttttgcgc caggaatgac attgcgttgc tgttcaccac
    14941 gcgtgcattg cctgctttta gtgtcgccag attcttaatt aactgctgag aaaccgcgcg
    15001 atccatctca atcaacgtgg cccccgcagc atagcgcgac aacgcttcca gccccagcgc
    15061 gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca atgaccggag ccagccagtt
    15121 aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga cctgggctat caggaaccgg
    15181 gagtttacgg cctcgccact gcccgccaat aatgcggatt tggccgctgc cggaatgatt
    15241 cggttttttc atgataaatt gctcaatccg ccagataaca catacttgca ggcggtgatg
    15301 tgaattaagt taagtgatag actatttcat cattttttta gctgctatgt acatagcgtt
    15361 aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca aatggcgaaa gaaaaaaaac
    15421 gtggcttttt ttcctggctg ggctttggtc aaaaagagca gaccccggaa aaagagacag
    15481 aagttcagaa tgaacaaccg gttgtagaag aaatcgttca ggcgcaagag cctgcgaagg
    15541 cttcggaaca agccgttgaa gagcagccgc aggcgcatac tgaggccgaa gcggaaactt
    15601 ttgctgccga cgttgtggaa gtcactgaac aggttgttga aagtgaaaaa gcgcaacctg
    15661 aagcggaagt tgttgcacag ccggaactgg tcgtagaaga aacgccggag ccagtggcta
    15721 tcgagcgtga agagctgccg ttgccggaag acgtcaacgc cgaagaggtt tcgccagaag
    15781 agtggcaggc cgaagcggaa accgtagaga ttgtcgaagc ggcggaagaa gaagcggcta
    15841 aagaagaaat caccgacgaa gagctggaag cacaggcgct ggctgccgaa gcagcagaag
    15901 aggcggtgat ggtggttcct ccggcagaag aagagcagcc ggtagaagaa atcgctcagg
    15961 agcaggaaaa accgaccaaa gaaggtttct tcgcgcgcct gaaacgcagc ctgttaaaaa
    16021 ctaaagaaaa tctcggttcc ggatttatca gcctgttccg cggtaaaaaa atcgacgatg
    16081 atctgtttga agagctggaa gaacagctgt tgatcgccga tgtgggcgtg gaaaccacac
    16141 gtaaaataat caccaatctg acggaaggcg catcccgcaa gcagcttcgt gatgccgagg
    16201 cgctttatgg cctgctgaaa gaagagatgg gcgagattct ggcgaaagtc gatgagccgc
    16261 tgaatgttga aggtaaaacg ccgttcgtga tcctgatggt gggcgtcaac ggtgtgggta
    16321 aaaccacgac gattggtaag ctggcacgtc agtttgagca gcagggtaaa tcggtgatgc
    16381 tggcggcggg cgatactttc cgtgcggcag cggttgaaca gcttcaggtc tggggtcagc
    16441 gcaacaatat tccggtgatt gcccagcata ccggggcgga ttccgcctct gttatcttcg
    16501 acgccattca ggcagctaaa gcgcgtaata tcgacgtcct gattgccgat accgccggac
    16561 gcctgcagaa caaatcgcac ctgatggaag agttgaagaa aatcgtccgc gtgatgaaga
    16621 aactcgacgt tgaagcgccg catgaagtta tgctgactat tgatgccagc accgggcaga
    16681 acgcggtaag ccaggccaaa ctgttccatg aagccgttgg cttaaccggc atcacgctaa
    16741 cgaaactgga cggcacggcg aaaggcggag taattttctc ggtggctgac cagtttggta
    16801 tccctatccg ctacattggt gtcggcgaac gtattgagga tttgcgtccg tttaaggcgg
    16861 acgactttat agaggcactt tttgcccgag aggattaaca atgattcgct ttgaacatgt
    16921 cagcaaggct tatctcggtg ggagacaggc gctgcagggc gttacgttcc atatgcagcc
    16981 gggtgagatg gcgtttctga ccggtcattc cggcgcaggg aaaagtaccc tcctgaagct
    17041 gatctgtggg attgagcggc ccagcgccgg gaaaatctgg tttagcggcc atgacatcac
    17101 gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag attggtatga ttttccagga
    17161 tcaccatcta ctgatggacc gtactgtcta cgataacgtg gcgatcccgc tgattatcgc
    17221 cggtgccagc ggtgacgata ttcgtcgccg ggtgtcggcg gcactggata aagtcgggct
    17281 actggacaaa gcgaagaact ttcctattca gctttccggc ggtgaacaac agcgtgttgg
    17341 cattgcccgc gcggtggtga ataagcccgc ggtactgctg gcggacgaac cgactggtaa
    17401 cctggacgac gcgttgtcgg aaggcatttt acgtctgttt gaagagttta accgcgttgg
    17461 ggtaaccgta ttgatggcaa cgcacgacat caacctgatt tcgcggcgtt cctatcgcat
    17521 gctcaccctg agcgatggtc acttgcatgg aggcgtgggc catgaataag cgcgatgcaa
    17581 tcaatcatat tcggcagttt ggcgggcgtc tggatcgctt ccgtaaatcg gtcggcggct
    17641 caggcgacgg cggtcgtaac gcaccgaaac gcgcgaaatc ctcgccaaaa ccggtaaatc
    17701 gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt ccacggcgca ttgcaggatc
    17761 tgaaaagcaa accgttcgcc acgtttttaa cggtgatggt tatcgccatc tccctgacgc
    17821 tgcccagcgt ctgttatatg gtgtacaaaa acgttaacca ggcggcgacg caatattatc
    17881 cgtcaccgca aatcactgtt tatctgcaaa aaacgctgga cgatgacgcc gctgcgggtg
    17941 tggtggcaca gttacaggcc gagcaaggcg tagagaaagt gaattatctt tctcgtgaag
    18001 acgcactggg cgagttccgt aactggtctg gttttggtgg tgcgctggat atgctggaag
    18061 aaaacccgct tccggcagtg gcggtggtga tcccgaaact cgattttcag gggacggaat
    18121 cactgaatac gctgcgtgat cgtatcaccc agatcaacgg cattgacgaa gtgcggatgg
    18181 atgacagctg gtttgcccgt ctggcggcgt taaccgggct ggtcgggcgc gtttccgcga
    18241 tgatcggcgt gttgatggtg gcagcagtgt tcctcgtcat cggcaacagt gtgcgtctga
    18301 gcatctttgc tcgccgtgac tccattaacg tgcagaaact gattggtgcg acggatggtt
    18361 tcatcctgcg cccgttcctg tacggaggcg cattgctggg attttctggc gcattgctgt
    18421 cattaatttt gtcagaaatt ctggtactgc gtttgtcatc ggcggttgcg gaagtggcac
    18481 aggttttcgg aacaaagttt gatatcaatg gcttatcgtt cgatgaatgc ctgctgttgc
    18541 tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct tgccacggta caacatttac
    18601 gccactttac gccggaataa taaaagcgtg ttatactctt tccctgcaat gggttccgta
    18661 gcagggaaag agaccccgtt gtctcttccc ggtatttcat ctctatgtca cattttgtgc
    18721 gtaatttatt cacaagcttg cattgaactt gtggataaaa tcacggtctg ataaaacagt
    18781 gaatgataac ctcgttgctc ttaagctctg gcacagttgt tgctaccact gaagcgccag
    18841 aagatatcga ttgagaggat ttgaatgact gacaaaatgc aaagtttagc tttagcccca
    18901 gttggcaacc tggattccta catccgggca gctaacgcgt ggccgatgtt gtcggctgac
    18961 gaggagcggg cgctggctga aaagctgcat taccatggcg atctggaagc agctaaaacg
    19021 ctgatcctgt ctcacctgcg gtttgttgtt catattgctc gtaattatgc gggctatggc
    19081 ctgccacagg cggatttgat tcaggaaggt aacatcggcc tgatgaaagc agtgcgccgt
    19141 tttaacccgg aagtgggtgt gcgcctggtc tccttcgccg tccactggat caaagcagag
    19201 atccacgaat acgttctgcg taactggcgt atcgtcaaag ttgcgaccac caaagcgcag
    19261 cgcaaactgt tcttcaacct gcgtaaaacc aagcagcgtc tgggctggtt taatcaggat
    19321 gaagttgaaa tggtggcccg tgaactgggc gtaaccagca aagacgtacg tgagatggag
    19381 tcgcgtatgg cggcacagga catgaccttt gacctgtctt ccgatgacga ttccgacagc
    19441 cagccgatgg ctccggtgct ctatctgcag gataaatcat ctaactttgc cgacggcatt
    19501 gaagatgata attgggaaga gcaagcggca aaccgtctga ccgacgcgat gcaaggtctg
    19561 gacgagcgta gccaggatat catccgcgcg cgctggctgg atgaagacaa caagtccacg
    19621 ttgcaggaac tggctgaccg ttacggtgtt tccgctgagc gtgtgcgcca gctggaaaag
    19681 aatgcgatga aaaaacttcg cgctgccatt gaagcgtaat ttccgctact cagcagagaa
    19741 ccctggatga gaatccgggg tttttgtttt ttgggcctct gcaataatca gtttccccct
    19801 ccggcaaaac gccaatcccc acgcagattg ttaataaact gtcaaaatag ctattccaat
    19861 atcataaaaa tcgggtatgt tttagcagag tatgctgcta aagcacgggt agtcatgcat
    19921 aaaacgaaat aaagtgctga acaacaacat cacaacacac gtaataacca gaagaatggg
    19981 gattctcagg atgaacataa agggtaaagc gttactggca ggatgtatcg cgctggcatt
    20041 cagcaatatg gctctggcag aagatattaa agtcgccgtc gtaggcgcaa tgtccggtcc
    20101 ggtggcgcag tacggtgacc aggagtttac tggcgcagag caggcggttg cggatatcaa
    20161 tgctaaaggt ggtatcaaag gcaacaaact gcaaatcgta aaatatgacg atgcctgtga
    20221 cccgaaacag gcggttgcgg tggcgaacaa agtcgttaac gacggcatta aatacgtgat
    20281 tggtcacctc tgctcttcat caacgcagcc agcgtctgac atctacgaag acgaaggcat
    20341 tttaatgatc accccagcgg caaccgcacc agagctgacc gcccgtggct atcagctgat
    20401 cctgcgcacc accggcctgg actccgacca ggggccgacg gcggcgaaat atattcttga
    20461 gaaagtgaaa ccgcagcgta ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct
    20521 ggcgcgagcg gtgcaggacg gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg
    20581 tatcaccgcc ggggaaaaag atttctcaac gctggtggcg cgtctgaaga aagagaatat
    20641 cgacttcgtt tactacggcg gttatcaccc ggaaatgggg caaatcctgc gtcaggcacg
    20701 cgcggcaggg ctgaaaactc agtttatggg gccggaaggc gtggctaacg tctcgctgtc
    20761 taacattgcg ggtgaatcag cggaaggact gctggtgacc aagccgaaga actacgatca
    20821 ggttccggcg aacaaaccca ttgttgacgc gatcaaagcg aagaaacagg acccaagcgg
    20881 cgcgttcgtc tggaccacct acgccgcgct gcaatctttg caggtgggcc tgaatcagtc
    20941 tgacgatccg gctgaaatag ccaaatacct gaaagcgaac tccgtggata cggtgatggg
    21001 gccgctgacc tgggatgaga aaggtgatct gaaaggcttt gagttcggcg tatttgactg
    21061 gcacgccaac ggcacggcga ctgatgcgaa gtaatcatta atcggcaact ttgggttgcc
    21121 gccaaattgc taatatcgag tacgttgctt catgccggat gcggcgtaaa cgccttatcc
    21181 ggcctacaag attcaaagaa atcagtaaat tgcagcacac attgtaggcc tgataagcgt
    21241 agcgcatcag gcaatatcac ttgtgaaatc aaacttgacg attaacgctt ctcccagccg
    21301 ccctgttgtg ccgtaaatcc cagtgcctgc ataaacgctg tcatcacgcc gcgatcttcc
    21361 acgcctgcat ccgccatcca ccagcatgaa acgccaggat tgttacgcaa aacctcttcc
    21421 agcagatatt gccccacacc gcgacggcgg gtgacttccc tcacacgcag ggaatccagt
    21481 gccccctcgg tgccgcttaa ggttacccgc acggccgcga gaaggcgctc gttaaaacgc
    21541 gcggcgtaga tacggtggtt atcgtcaacc tgtaaagagg aaggggaata ctccggccag
    21601 atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt ctaatcgaat gatggtcagc
    21661 ttcatgggga acccgtgtaa atcacaaaag tggaaccagt gtagcgaaat aatttaatcg
    21721 gaggctttc
//
LOCUS       DAZKJR010000086        21419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKJR010000086 DAZKJR010000000
VERSION     DAZKJR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.086"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2739
                     /locus_tag="ACHOWS_003253"
     CDS             <1..2739
                     /locus_tag="ACHOWS_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HFQ0048785.1"
                     /translation="DSLLWNATAGAFSAKHGTNGTDSKITNLLAGTVSSDSTDAINGS
                     QLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGDALAAINTSFSTSLGDA
                     LLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTSSDAINGSQLYDTSKYIADT
                     LGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDALLWDATANDGNGAFSA
                     AHGKDKTASVITNVANGAISATSSDAINGSQLYTTNKYIADALGGDAEVNADGSITAP
                     TYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHDGKASIITNVADGNIGE
                     GSTDAINGSQLFNTNMMIQQNSEIINQLAGNTSETYIEDNGAGINYVRTNDNGLAFND
                     ASASGIGATAVGYNSVAEGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIVKGSRDTSV
                     SEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVAT
                     TPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSN
                     ARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGS
                     ADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVSTGSTKYF
                     KTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEENTISVGSSTNQRRITN
                     VAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSVDYSNITLGGGNGGTTRISNVSAGV
                     NNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYT
                     PGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3151..4806
                     /gene="lldP"
                     /locus_tag="ACHOWS_003254"
     CDS             3151..4806
                     /gene="lldP"
                     /locus_tag="ACHOWS_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFQ0048786.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4806..5582
                     /gene="lldR"
                     /locus_tag="ACHOWS_003255"
     CDS             4806..5582
                     /gene="lldR"
                     /locus_tag="ACHOWS_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFQ0048787.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5579..6769
                     /gene="lldD"
                     /locus_tag="ACHOWS_003256"
     CDS             5579..6769
                     /gene="lldD"
                     /locus_tag="ACHOWS_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFQ0048788.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIREFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            6842..7315
                     /gene="trmL"
                     /locus_tag="ACHOWS_003257"
     CDS             6842..7315
                     /gene="trmL"
                     /locus_tag="ACHOWS_003257"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0048789.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7368..8189)
                     /gene="cysE"
                     /locus_tag="ACHOWS_003258"
     CDS             complement(7368..8189)
                     /gene="cysE"
                     /locus_tag="ACHOWS_003258"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFQ0048790.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8269..9288)
                     /gene="gpsA"
                     /locus_tag="ACHOWS_003259"
     CDS             complement(8269..9288)
                     /gene="gpsA"
                     /locus_tag="ACHOWS_003259"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0048791.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9288..9755)
                     /gene="secB"
                     /locus_tag="ACHOWS_003260"
     CDS             complement(9288..9755)
                     /gene="secB"
                     /locus_tag="ACHOWS_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFQ0048792.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9818..10069)
                     /gene="grxC"
                     /locus_tag="ACHOWS_003261"
     CDS             complement(9818..10069)
                     /gene="grxC"
                     /locus_tag="ACHOWS_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFQ0048793.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10211..10642)
                     /locus_tag="ACHOWS_003262"
     CDS             complement(10211..10642)
                     /locus_tag="ACHOWS_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0048794.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10887..12431
                     /gene="gpmM"
                     /locus_tag="ACHOWS_003263"
                     /gene_synonym="pgmI"
     CDS             10887..12431
                     /gene="gpmM"
                     /locus_tag="ACHOWS_003263"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0048795.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12465..13724
                     /gene="envC"
                     /locus_tag="ACHOWS_003264"
     CDS             12465..13724
                     /gene="envC"
                     /locus_tag="ACHOWS_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFQ0048796.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13728..14687
                     /locus_tag="ACHOWS_003265"
     CDS             13728..14687
                     /locus_tag="ACHOWS_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFQ0048797.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(14674..15708)
                     /gene="waaH"
                     /locus_tag="ACHOWS_003266"
     CDS             complement(14674..15708)
                     /gene="waaH"
                     /locus_tag="ACHOWS_003266"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFQ0048798.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(15947..16972)
                     /gene="tdh"
                     /locus_tag="ACHOWS_003267"
     CDS             complement(15947..16972)
                     /gene="tdh"
                     /locus_tag="ACHOWS_003267"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0048799.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARYVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(16982..18178)
                     /gene="kbl"
                     /locus_tag="ACHOWS_003268"
     CDS             complement(16982..18178)
                     /gene="kbl"
                     /locus_tag="ACHOWS_003268"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0048800.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18392..19324
                     /gene="rfaD"
                     /locus_tag="ACHOWS_003269"
     CDS             18392..19324
                     /gene="rfaD"
                     /locus_tag="ACHOWS_003269"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFQ0048801.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19334..20380
                     /gene="rfaF"
                     /locus_tag="ACHOWS_003270"
     CDS             19334..20380
                     /gene="rfaF"
                     /locus_tag="ACHOWS_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFQ0048802.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20384..21364
                     /gene="rfaC"
                     /locus_tag="ACHOWS_003271"
     CDS             20384..21364
                     /gene="rfaC"
                     /locus_tag="ACHOWS_003271"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFQ0048803.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKAAMI"
BASE COUNT         5210 a         5663 c         5613 g         4933 t
ORIGIN      
        1 gattccctgc tgtggaacgc tacggcgggt gcattcagtg ccaaacacgg caccaacggt
       61 actgacagca aaatcaccaa cttactggca ggcactgtat cctctgatag cactgacgcc
      121 attaatggct cacaacttta tggtttagct gattcattta cgtcatacct tggcggtggt
      181 gctgatatca gcgatgcggg tgtattaacc gggccaacct acactattgg tggtactgac
      241 tacaataacg tcggtgatgc tctggcagcc attaacacgt catttagcac atcactcggt
      301 gacgccctac tctgggatgc gaccgccaag ggcggtgacg gtgcgtttag cgccggtcgc
      361 ggaaaagata acacggccag catcattact aatgttgcag acggtgcaat ttcctctacc
      421 agcagcgacg ctattaacgg ctcacagctc tatgacacca gtaagtacat tgccgatact
      481 ctgggtggtg acgcagaagt caatgctgac ggcacaatca ccgcaccgac ttatgctatc
      541 gctggcggca gttacagcaa cgtcggtgac gcgctggaag caatcgatac cacgctcgat
      601 gacgctctgt tgtgggatgc cacagccaat gatggtaatg gtgcatttag cgccgctcac
      661 ggaaaagata aaacagccag tgtaatcact aacgtcgcta acggtgcaat ctctgccacc
      721 agcagcgatg ccattaacgg ttcacaactg tataccacca acaagtacat tgccgatgcc
      781 ctgggtggtg acgcagaagt taacgctgat ggttctatca ctgcgccgac ttacaccatt
      841 gcaaatgccg agtacaacaa cgtcggtgac gccctggatg cgctcgacga taacgctctg
      901 ctgtgggatg caacggccaa tgacggcgca ggtgcctaca acgccagcca tgacggcaag
      961 gccagcatca tcacaaatgt tgctgatggt aacattggcg aaggcagtac cgacgccatc
     1021 aacggttctc agctgtttaa caccaatatg atgatccagc agaacagcga aatcatcaat
     1081 cagcttgccg gtaacaccag cgaaacctac atcgaagata acggtgcggg tattaactat
     1141 gtacgtacca acgacaacgg cttagcgttc aacgatgcca gcgcttcagg tattggcgct
     1201 acggctgtgg gttataactc tgttgccgaa ggcgatagca gcgtagccat cggtcagggc
     1261 agctacagcg acgttgatac cggtattgcc ctgggtagca gctccgtttc cagccgtgta
     1321 atagttaaag gttctcgtga caccagcgtg tcggaagaag gtgttgtgat tggttatgac
     1381 accacggatg gcgaactact tggtgcgctg tctattggtg atgacggtaa atatcgtcaa
     1441 atcatcaacg tagccgatgg ttccgaagcc catgacgccg ttacggttcg ccagttgcaa
     1501 aatgccattg gtgcagtcgc taccacgccg accaaatact tccacgccaa ctcaacggct
     1561 gaagactcac tggctgtcgg ggaagactcg ctggcgatgg gcgcgaaaac tatcgtcaat
     1621 ggtaatgcgg gtattggtat cggcctgaat actctggttc tggcagatgc catcaacggt
     1681 attgctatcg gttctaacgc acgcgccaac catgcaaaca gtattgcaat gggtaatggt
     1741 tctcagacca ctcgcggcgc tcagactggc tacaccgcct acaacatgga tgcaccgcag
     1801 aactctgtgg gtgaattctc cgtaggtagc gaagacggtc aacgtcagat caccaacgtc
     1861 gcagcggggt cggcggatac cgatgcggtt aacgtgggtc agttgaaagt aacggacgcg
     1921 caggtttccc agaataccca gagcattact aacctgaata ctcaggtaac gaatctggat
     1981 acccgcgtca ccaatatcga aaacggtatt ggcgatatcg taagcaccgg tagcaccaag
     2041 tacttcaaga ccaacaccga tggcgtagat gctaacgcgc agggtaaaga cagtgttgcg
     2101 attggctctg gctctattgc tgccgctgat aacagcgttg cgctgggtac tggctctgtg
     2161 gcaaacgaag aaaatacgat ctccgtaggt tcttccacta accaacgccg tatcaccaac
     2221 gttgccgctg gtaaaaatgc tactgacgcg gttaacgttg cgcagttgaa gtcttccgaa
     2281 gcaggcggcg tgcggtatga caccaaagct gacggttctg ttgattacag caacatcact
     2341 ctcggcggtg gtaatggcgg aacgactcgc atcagcaacg tttccgctgg cgttaacaac
     2401 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
     2461 gatcagcgca tggttgagat ggataacaaa ctgtccaaaa ccgaaagcaa gttgagcggt
     2521 ggtatcgctt ctgcaatggc gatgaccggt ctgccgcagg cttacacgcc aggtgccagc
     2581 atggcctcta ttggtggcgg tacttacaac ggtgaatcgg ctgttgcttt aggtgtgtcg
     2641 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
     2701 gaatactccg ccgcgctcgg tgccggtatt cagtggtaat catccattaa cgaatgggta
     2761 aacgccacat tgcctgatgc gctacgctta tcaggcctac ggggtgattc agcactaccc
     2821 tgtaggccgg ataaggcgtt cacgccgcat ccggcaagat aaggcgcacc agatcaacaa
     2881 tctgagggca attctctgat gggaattgcc cttttcttca ttacctctct cccagaattg
     2941 gccctaccaa ttcttcattg atctgacctc tggttcacaa ttccccaatt aaaactcaca
     3001 tcaccgttgc caatacataa catctagtta actattcatt gtcattatcc ctacacaaca
     3061 caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac
     3121 acggcgtgtg tggactgcag gagacctgca atgaatctct ggcaacaaaa ctacgatccc
     3181 gccgggaata tctggctttc cagcctgata gcatcgcttc ccatcctgtt tttctttttt
     3241 gcgctgatta agctcaaact gaaaggatac gtcgccgcct catggacggt ggcaatcgcc
     3301 cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtgatt
     3361 tatggcttct tctacggttt gtggcccatc gcgtggatca ttattgcggc agtgttcgtc
     3421 tataagatct cggtgaaaac cgggcagttt gacatcatcc gctcgtctat tctttcgata
     3481 acccctgacc agcgcctgca aatgctgatc gtcggtttct gtttcggcgc tttccttgaa
     3541 ggagccgcag gctttggcgc tccggtagca attaccgccg cattgctggt cggtctgggc
     3601 tttaaaccac tctacgccgc cggactgtgc ctgattgtta acaccgcgcc agtggcattt
     3661 ggtgcgatgg gtattccgat tctggtcgcc gggcaggtaa caggcatcga cagctttgag
     3721 attggtcaga tggtggggcg tcagttgccg tttatgacca ttatcgtgct gttctggatc
     3781 atggcgatta tggacggttg gcgcggaatc aaagaaacgt ggcctgcggt cgtggtggca
     3841 ggtggttcgt ttgccattgc tcaatacctc agctctaact tcattgggcc ggaactgcca
     3901 gacattatct cttcgctggt atcactgctc tgcctgacgc tgttcctcaa acgctggcaa
     3961 ccagtgcgcg tattccgttt cggtgacttg ggggcgtcac aggttgatat gacgctggct
     4021 cacaccggtt acaccgcagg tcaggtgctt cgcgcctgga caccgttcct gttcctgacc
     4081 gccaccgtaa cgctgtggag tatcccgccg tttaaagccc tgttcgcttc aggcggtgcg
     4141 ctgtatgagt gggtgatcaa cattccggtg ccgtacctcg ataaactggt tgcccgtatg
     4201 ccgccagtgg tcagcgaagc gacagcgtat gccgccgtgt ttaagtttga ctggttctct
     4261 gccaccggta ccgccattct ttttgctgcc ctgctctcaa ttgtctggct gaagatgaaa
     4321 ccatctgacg ctatcagcac cttcggcagc acgctgaaag aactggctct gcctatctac
     4381 tccatcggta tggtgctggc gttcgccttt atctcgaact attccggact atcatcaacg
     4441 ctggcgctgg cactggcgca caccggccat gcattcacct tcttctcgcc gttcctcggc
     4501 tggcttggtg tcttcctgac cggatcggat acctcatcta acgccctgtt cgccgccctg
     4561 caagccactg cagcacaaca aattggcgtt tctgacctgt tgttggttgc cgccaacacc
     4621 accggtggtg tcaccggtaa gatgatctct ccgcaatcta tcgctatcgc ctgtgcggcg
     4681 gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc
     4741 ttcacctgta tggtgggcgt gatcaccacg cttcaggctt atgtcttaac gtggatgatt
     4801 ccttaatgat tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc
     4861 tgattgatga aaaaaacctg gaagcgggca tgaagttgcc cgctgaacgt caactggcga
     4921 tgcaactcgg cgtgtcgaga aattcactgc gcgaggcgct ggcgaaactg gtcagtgaag
     4981 gtgtgctgct cagtcgacgt ggcggcggga cgtttattcg ctggcgtcat gatacatggt
     5041 cggagcaaaa catcgtccag ccgctgaaaa cgctgatggc cgatgatccg gattacagtt
     5101 tcgatattct ggaagcccgc tacgccattg aagccagcac cgcgtggcat gcggcaatgc
     5161 gcgccacacc aggcgaaaaa gagaaaattc agctttgctt tgaagcaacg ctaagtgaag
     5221 acccggatct cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac
     5281 ataacatcgt gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctctgtga
     5341 agcatagccg ccagcggatg tatctggtac ccccggtttt ttcacagctg accgaacaac
     5401 atcaggcagt cattgacgcc atttttgccg gtgatgctga cggggcgcgt aaagcaatga
     5461 tggcgcacct tagctttgtt cacaccacca tgaaacgatt cgatgaagat caagctcgcc
     5521 atgcacgcat tacccgcctg cctggtgacc ataatgagca ttcgagggag aaaaacgcat
     5581 gattatttcc gcagccagcg attatcgcgc cgcagcacaa cgcattctgc cgccgttcct
     5641 gttccactat atggatgggg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga
     5701 tttgtcagaa gtggcgctgc gccagcgtat tctgaaaaac atgtctgact taagcctgga
     5761 aacgacgctg tttaatgaga aattgtcgat gccggtggcg ctaggtccgg taggtttgtg
     5821 tggcatgtat gcgcgacgcg gcgaagttca ggctgccaaa gccgcggatg cgcatggcat
     5881 tccgtttact ctctcgacgg tttccgtttg cccgattgaa gaagtggctc cagccattaa
     5941 acgtccgatg tggttccagc tttatgtgct gcgcgatcgc ggctttatgc gtaacgcact
     6001 ggagcgagca aaagcggcag gctgctcgac actggttttc accgtggata tgccgacacc
     6061 gggcgcgcgt taccgtgatg cacactcggg tatgagcggc ccaaatgcgg caatgcgccg
     6121 ttacttgcag gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc
     6181 acatgattta ggtaatatct cggcttacct cggcaaaccg accggactgg aagattacat
     6241 cggctggctg gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg
     6301 cgaattctgg gatggtccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga
     6361 tgcagtacgt tttggtgctg atgggattgt ggtttctaac cacggtggcc gccagctgga
     6421 cggtgtactc tcttccgctc gtgcattgcc cgctattgcg gatgcggtga aaggtgatat
     6481 tgccattctg gcagatagcg gaatacgtaa cgggcttgat gtcgtgcgta tgattgcgct
     6541 cggtgccgac accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca
     6601 ggcgggcgtg gcaaatctgc tgaatctgat cgaaaaagag atgaaagtgg caatgacgct
     6661 gactggcgcg aaatcgatta gcgaaattac gcaagattcg ctggtgcagg ggctgggtaa
     6721 agagttgccg gcggcactgg ctccgatggc gaaagggaat gcggcatagt tgggggtgag
     6781 gggaaaaggc cgtaccaatc accaattttc tgctatcctg cccccgcact aagggggcag
     6841 tatgctaaac atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg
     6901 tctttgcgct aataccggct ttcgtctgca tatcatcgaa ccaatgggat ttgcctggga
     6961 cgataagcgc ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca
     7021 tcatgactat cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac
     7081 gaaaggtacg cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg
     7141 cccggaaaca cgcggcctgc cagcgagcat tcttgatgcc ctgcccactg aacaaaaaat
     7201 tcgtattcca atggtgccgg acagccgcag catgaacctg tctaatgcgg tgtcggtcgt
     7261 ggtgtatgaa gcctggcggc agttggggta tccgggagcg gtgttgagag attagttact
     7321 gtaggccgga tagatgatta catcgcatcc ggcacgatca caggacatca gatcccatca
     7381 ccatactcaa acgtatggtt aatgccgttg aaatgctgat ccatatccat tgacggttta
     7441 tcgctatccg gcttgccgac aatgcgtgcc ggaacgccag cggcagtggt gtgcggcggc
     7501 accggttgca gcaccacgga acctgcgcca atcttcgcgc cgcgcccaac ttcaatatta
     7561 ccgagaattt tcgcgcccgc gccaatcatc acgccttcac gaattttcgg gtgacggtca
     7621 ccaccagatt tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca
     7681 atcaccgccg tttcacccac aacgatgccc gtcgcgtggt cgagcatgat accgcgacca
     7741 atttttgctg ccgggtgaat atcaacctgg aacgttacag agacctggtt ttgtagaaaa
     7801 atagccagcg cgcgacggcc ctgattccac aaccagtgac cgatgcgata cgcctgcaaa
     7861 gcatgaaaac ccttcaggta tagcaacggg gttgagtatt tatccaccgc cgggtcacgg
     7921 gtacgcaccg cctgaatatc acaggccgca gaggcgatca tttccgggtc ggcggcgtag
     7981 gcttcttcca ccacttcacg gatagcaata gccggcataa tgggcgatga cagcttgttc
     8041 gccagcatgt agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa
     8101 aaactggcca gcattggctc acagtctgcc agcgttctgg cttcagcttt aatattgttc
     8161 cagacaattt ccagttcttc acacgacatt gcttactcca cacgataaga taatgaccgg
     8221 cccgttctgc gcgggccggg tcatagtggt aacaaaggtt ccctgggttt agtggctgct
     8281 gcgctcgtcc ttgcgtgcac gacctagtaa agtcaatgct gcctcgcgcg cgttttttcc
     8341 gcaatataat acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc
     8401 cagttcgcgg acttctttcg tattgcggta gccttccacc acttgaccaa ttttctcctg
     8461 cgcgctttgt acatccatgc cctgaccgag catcatgcca aaacggcggt tacgcgactg
     8521 gttgtcggta caggtcagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg
     8581 atcggcaccc agcgccgcgc caagacgcga catctcagcc agcccacggg tgatcagcgc
     8641 cgtacgcgca ttcgcgccaa aaccgatacc gtcggacatc cccgcgccaa tggcaatgac
     8701 gtttttcacc gcgccgccaa gctgcacacc gatgaaatcc gggttgctgt aaacgcggaa
     8761 acttttgcca cagtgcagca attgctggag gtcatcggca aaggtctggt ccgtcgaggc
     8821 cagcgaaatg gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat
     8881 aaccgccagc ggaatttgat cgcctaaggc ctcacgcgct acgtcctgta acagacgtcc
     8941 ggtttctgct tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg
     9001 tttaatctgg cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg
     9061 gctggctgcc agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac
     9121 atcggggaga aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc
     9181 atggccccag aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc
     9241 gtacgagccg gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga
     9301 tgttcttcag taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag
     9361 ttaaccggcg caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac
     9421 tcacgagcat acgggaacag aatgttcggg cagtatgcac ccaggcaatg tgccatctgg
     9481 gtgccttcga tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg
     9541 gtttcttcgc ccagggaggc cgttacggta acacgcagta ccacttcgta aacgtcatct
     9601 gccagttgag aagatgccgt atccagatca agtttaactt ctggctgcca atctttctgg
     9661 aaaacgtgcg gcgcgttcgg cgcttcgaaa gaaatatcct tggtataaat acgttggatc
     9721 tggaaagtca tttcggtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc
     9781 cttaaatact gcgcaatgta atgccaacgt tcacacgtta tttcagcagg ggatccagtc
     9841 caccacgtgc atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa
     9901 aaatctgggg aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc ttagcggcgt
     9961 tgccatcgat cggcaactcc tgaaaactca cgcccttgct gctcagcagt gctttcgcac
    10021 gatggcaata cgggcaggtt tctttggtat agatttcaac attggccata acttagctcc
    10081 tgttttttta ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa
    10141 gcttataaca ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac
    10201 gacaggtaaa ttatttgccg cgcaccagag gtaagttttc gccagcccag ccagcaacgc
    10261 cctctttcag cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct
    10321 ggcactgcat gccagaaccg tctaccacga taaccggttt gtctttgtgc ttctcaagct
    10381 caccaacatt gttggctttg atttcgctcg gcaacaggtt gatagaacct gcgatatggc
    10441 ctttacggaa gtcatcacgc tgacgtaaat ccacaaccac agcatcttct ttgttgatca
    10501 gacgcgtagc ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag
    10561 tcacaataac cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa
    10621 caaattgcat aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc
    10681 aggaaaggag tatacctgcg cggtgcagca aatacagcca gcgcgttaac tggaatgcaa
    10741 ttttgcgggg cgcgacgaaa aaaaagcgca aatctggcat cggcttgctg cgagcggtcg
    10801 tatattttga tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt
    10861 tgactcttga gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga
    10921 ttctggatgg ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa
    10981 ccccggtaat ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc
    11041 tggaagtcgg tctgcctgac cgtcagatgg gtaactccga agtaggtcac gttaacctgg
    11101 gtgccggccg catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg
    11161 ctttcttcgc taacccggta ctgactggcg cggtagataa agcgaaaaac gcaggcaaag
    11221 cagttcacat tatgggtctg ctctccgcag gtggtgtaca cagccacgaa gatcacatca
    11281 tggcgatggt agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc
    11341 ttgacggtcg cgacactccg ccgcgcagtg ctgaatcctc gctgaaaaaa ttcgaagaga
    11401 aatttgccgc actgggcaaa ggccgcgtag cgtccatcat tggtcgttac tacgccatgg
    11461 accgcgataa ccgttgggat cgcgttgaaa aagcttatga cctgctgact ctggcgcagg
    11521 gcgagttcca ggccgatacc gccgttgctg gtttgcaggc tgcttatgct cgcgacgaaa
    11581 acgacgaatt cgtgaaagcg accgttattc gtgcagaagg tcagccagac gcggcaatgg
    11641 aagacggcga tgcgctgatt ttcatgaact tccgtgctga ccgtgcacgt gaaatcactc
    11701 gtgcattcgt aaatgccgat tttgacggct tcgcgcgtaa gaaagtggtt aacgtcgatt
    11761 tcgtgatgct gaccgaatac gctgccgaca tcaaaaccgc agtggcttac ccacccgctt
    11821 ccctggttaa caccttcggc gagtggatgg cgaaaaacga caaaactcag ctgcgtattt
    11881 ccgaaaccga aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt
    11941 tcaaaggcga agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac
    12001 cggaaatgag ctccgcagag ctgaccgaaa aactggttgc ggcaatcaag agcggcaaat
    12061 acgacaccat tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggagtgatgg
    12121 aagcggcggt taaagcggtt gaagcgctgg atcactgcgt ggaagaagtc gcgaaagcgg
    12181 ttgagtccgt gggtggacaa ctgctgatca ccgctgacca cggtaacgct gagcagatgc
    12241 gcgatccggc aacgggtcag gcacacacgg cacataccaa cctgccagtt ccgctgattt
    12301 acgttggcga taagaacgtg aaagcggttg aaggcggcaa actttctgat atcgcgccga
    12361 ccatgttgtc gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca
    12421 tcgtggaata atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga
    12481 aaccgcgcag gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat
    12541 tgttgtgcgc cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg
    12601 atatcgccgc gaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg
    12661 cacaattgaa aaagcaggaa gaagcgatct cggaagccac ccgtaagctg cgtgaaacgc
    12721 aaaacacgct caatcaactg aataaacaga ttgatgagat gaacgcgtcg attgccaaac
    12781 tggagcagca aaaagccgcc caggagcgca gcctcgccgc acaactagat gccgcattcc
    12841 gtcagggcga gcataccggt attcagctga ttctcagcgg tgaagaaagc cagcgtggac
    12901 agcgtttaca ggcttatttc ggctatctca accaggcgcg acaagaaacc attgctcagt
    12961 tgaaacaaac gcgtgaagaa gtcgctatgc aacgcgccga gctggaagag aaacagagcg
    13021 agcaacaaac gctgttgtat gagcagcgcg cccaacaggc gaagctgacc caggcgctga
    13081 acgagcgtaa aaagacgctg gcagggctgg agtcttccat ccagcaaggt cagcaacagt
    13141 tgagcgagct gcgcgccaac gaatcccgtc tgcgtaacag cattgcccgt gcggaagccg
    13201 cggcgaaagc gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gaccgccaga
    13261 aagaagcgac gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt
    13321 cccgaactgg tggcctgggt gcgccgcgtg gtcaggcatt ctggccagtt cgcgggccga
    13381 cgctgcatcg ctatggcgag cagctacagg gtgaattacg ctggaaagga atggttatcg
    13441 gtgcctctga aggtactgaa gttaaagcca ttgccgacgg tcgggtgatt ctggctgact
    13501 ggctgcaagg ctacggtctg gtggtggtgg ttgagcatgg taaaggcgac atgagtcttt
    13561 acggctataa tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccga
    13621 ttgcactggt gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc
    13681 gccagggtca ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc
    13741 gtcgtaacgt tcttgcattt gccgctctgt tggcgctctc ctcccccgta attgctggca
    13801 agcttgccat cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg
    13861 cgatgccttc cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg
    13921 caaccaaagc gcataacagc ggtcacgaag tgttgattca tctaccgatg gcaccactga
    13981 gtaaacagcc gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc
    14041 gcattattcg tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg
    14101 gcagcaagat gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt
    14161 acaatcttta cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgcagcgc
    14221 aaggcactgg cgtgaaggtg atcaaacgga aagtgttcct cgacgattcg caaaatgaag
    14281 cggacatccg tgtgcagttc aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca
    14341 ttgccattgg gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc
    14401 tgccgccaga cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata
    14461 cgtctacacc gccgaaaaac actgtgcccg acacaccgcg taatccgttc cgtggcgtga
    14521 agctctgcaa accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat
    14581 taagcgaaag catcagccag agcacgctga tcgtttactt ccaacatcag tggcaaggct
    14641 ggggcaaaca gcctgaagcg gcaaaactta acgctagcgt aaattaaagg cgctacgcgt
    14701 aatgcgatgg tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa
    14761 cgcctgataa cccaccttca cgctgcgcac attggtaatc aggcgcttat acataccgga
    14821 agtaaatatc tcggcaatca tccgttggcg ggtaataata tccggctctt tgcgcaccgc
    14881 atggcaaaca cgcaatgctt cgtaagttat ttgctgatga aattccggat aaatcgtaat
    14941 tttgtcggca taatttcgat ttaatttctc tagcaggcgg gtaatcttaa tatagtgacg
    15001 ttgataatta aggtttttat tcccttgtct attcaaccga ctcactgacg tattatgcag
    15061 ataatattta tataacgatt gctcggtata tcgcgcacgc agcgcattaa acataaattc
    15121 tgttgtccag acaatatcct gatgatgtaa tccggcaata aatttaatgt tatttttaac
    15181 aataacatcg cggcgataaa cccccatcca gacaacgtga gtccaacggc gggaagaaag
    15241 ccccatccgc aaccaatccg ggccggttaa tacgccggtt gagcgaaggc gatcggtggg
    15301 gatggattgc caggtttctc ccgtttcacg aaaacaccag tcagcgttgc actgcgccac
    15361 gtcgaggtcg tcctctaacg ccatggtcat cagcgtttcg tacatggtgg gatagacctc
    15421 atcgtcagca tcgacaaaag cgacatattt gcccgtcgcc acctcgatcc cacgattacg
    15481 cgctaccgat gcgccagcat tcgcctgatg caacaaacga acgtgcggat agttttctgc
    15541 gtaatgcttt gctatttcaa cagaattatc cgttgaacca tcgttaataa taatgatttc
    15601 cagagcagtc caggtttgcg taattaaaga ttccatacac gtgcggaaat catcgcccgc
    15661 attatataac gggataataa cactaagttt attggtgctg ttcatcataa attaccgacg
    15721 acctctgaag ttatcctgac gttttacagg ctttgtatta agaaactatt aagcctgtga
    15781 gatattattt ttacaggtga gagagtcact tcagggtaga gagataatga gaacagctat
    15841 ataagagatc agcgcaatta cgaactcttt attacactga caggatttta catatttaac
    15901 ggattatcct caggaaaaag ggctggaata ccagcccttg tttgtgttaa tcccagctca
    15961 ggataacttt cccggactgg cccgaacgca tggcgtcaaa gcccttctgg aaatcatcga
    16021 tagagaaacg atgagtaatg atcggcgaaa gatcgaggcc agactgaatc agcgccgcca
    16081 tcttgtacca ggtttcaaac atctcacgac cgtaaatacc tttaatgaac aagcctttaa
    16141 agatcacttt ggtccagtct atcgacatgt cagaaggtgg aatacccagc atcgcaatac
    16201 gaccgccgtg gttcatggtg tcaagcatgg tacgaaacgc tggcggcgca ccggacattt
    16261 ccagaccgac atcaaagcct tcggtcatgc ccagttctgc catcacatca ttgaggttct
    16321 ctttagcgac gttaaccgca cgggtgatac ccattttacg cgccaactca aggcggtatt
    16381 cgttaacatc agtaatcacc acgtaacgtg caccaacgtg tttcgccacc gccgccgcca
    16441 taataccaat cgggcctgca ccagagacca gcacatcctc gcccaccaga tcgaacgaca
    16501 gcgcggtatg cacggcgtta ccgaagggat caaaaatgga agccagatca tcggagatat
    16561 tgtcggggat tttgaaagcg ttgaacgccg gaatcaccag atactcggcg aagcagcccg
    16621 gacggttaac gccgacgccg atcgtgttgc ggcacagatg ggtacgaccg ccacgacagt
    16681 tgcgacagtg accgcaggtg atatggcctt cgccagaaac gcgatcgcca atcttgaagc
    16741 ctttcacttc ctgaccaata cctaccactt caccgacata ttcatgaccc acgaccatcg
    16801 gcaccgggat ggttttttgc gaccactcat cccagttata gatgtgaacg tcagtcccgc
    16861 agatggctgt tttacggatt ttaatcagca gatcgttatg cccgagttcc ggtacaggaa
    16921 cgtcggtcat ccagatgccc tcttccgctt tcagtttgga taacgctttc atctcacatc
    16981 ctcaggcgat aacgcccagt tgtttaccaa tacgcgtgaa tgcttctact gcacgcgtaa
    17041 tttgctcagg ggtatgcgcc gcagacatct gggtacgaat acgcgcctga cctttcggaa
    17101 cgaccggata gaagaaaccg gtgacgtaaa tcccctcttt ttgcagctca cgggcaaatt
    17161 tctgcgccac taccgcatcg ccaagcatga ccggaataat ggcgtgatcg gctcccgcca
    17221 gggtaaagcc cgccgccgac atttgctcac ggaactgacg cgcgttcgcc cacagacggt
    17281 cacgcagctc gctacccgct tcaaccatct ccagcacttt gatggaagcg gcaacaatgg
    17341 ccggtgccag cgagttggag aacaggtacg gacgagaacg ctggcgcagc cactcaacca
    17401 cttctttgcg cgccgcggta taaccaccag aagccccgcc cagcgcttta ccaagcgtac
    17461 cggtgataat atcgacccgc cccatcacat cgcagtattc atgggaacca cgaccgtttt
    17521 caccgacaaa acctaccgcg tgtgagtcat ctaccatcac cagggcatca tatttatctg
    17581 ccagatcgca aacgcctttc aggttggcaa tcacgccgtc catcgagaac acaccatcgg
    17641 tggcaatcag cacatgacgc gcaccggctt cacgcgcttc tttcagacgt gcttccagct
    17701 cctgcatatc gttgttggca taacgatagc gtttagcttt gcacagacgc acaccatcaa
    17761 taatagacgc gtggttcagt gcgtcggaga taatggcgtc ttccgcgccc aacagcgttt
    17821 cgaacaggcc accgttagca tcaaagcagg aagagtagag aatcgcatct tccatcccca
    17881 ggaaggccgc cagtttttgt tcaagctctt tatggctgtc ctgagtgccg caaataaaac
    17941 gcaccgaagc catgccgaaa ccgtgagaat ccattcccgc ctttgccgcc gcaatcagat
    18001 ccggatgatt cgccagtccg agatagttat tggcacaaaa gttaatgacg tggcttccat
    18061 cagccacagt gatatctgct tgctgcgcag acgtaataat gcgctcttct ttaaacaacc
    18121 cttccgcccg tgcggtttcc agatcgttgg ttaactgctg ataaaattct ccacgcattg
    18181 cgattctcca gacagggcaa attccagcac atattaccca aacttatagg ttgcgacgag
    18241 atgacgcgtt aacacttctg caaaattcag gataaataac gtccagcccc ctcacttttg
    18301 gtgaatggct gaagccaacg tcatgtagtg atatgataga agtattcgtg tctgagattg
    18361 tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg gcgcgggctt
    18421 tatcggaagc aacatcgtta aagccctgaa tgataaaggc atcaccgata ttctggtggt
    18481 ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctggata tcgcggacta
    18541 tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg gcgatgtcga
    18601 agcgattttc cacgaaggcg cgtgctcttc caccaccgag tgggacggca agtatatgat
    18661 ggataacaac tatcaatact ccaaagagct gctgcactac tgtctggagc gcgaaatccc
    18721 gttcctgtat gcctcttccg cagccactta cggtggacgc acctccgact ttattgaatc
    18781 ccgcgaatat gaaaaaccgt tgaatgtcta cggttactca aaattcctgt ttgatgaata
    18841 tgttcgccaa atcctgcctg aagcgaactc acagattgtt ggcttccgtt atttcaacgt
    18901 ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc atctcaacac
    18961 ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact tcaaacgtga
    19021 cttcgtctat gtgggcgatg tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc
    19081 cggcatcttc aacctcggta ctggtcgtgc ggaatccttc caggcagtag cagatgctac
    19141 gcttgcttat cacaagaaag gccaaatcga atacattccg ttcccggata aactgaaagg
    19201 ccgctaccag gcgttcacgc aggcagatct gacaaatctg cgcgcggcgg gttacgacaa
    19261 accgtttaaa accgttgccg aaggcgtaac ggaatacatg gcctggctga atcgtgacgc
    19321 ataagagctc tgcatgaaaa tactggtgat cggcccgtct tgggttggcg acatgatgat
    19381 gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa tcgacgtgat
    19441 ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg aagctattcc
    19501 tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct
    19561 gcgcgaaaaa cgttacgacc gcgcctacgt tttacccaac tccttcaaat ccgcattagt
    19621 gcctttcttc gcgggtattc ctcatcgcac cggctggcgc ggcgaaatgc gctacggttt
    19681 actcaacgat gtgcgtgtgc tggataaaga agcctggccg ctgatggtgg aacgctatgt
    19741 ggcgctggcc tatgacaaag gtattatgcg taccgcacaa gatctgccgc agccattgtt
    19801 atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc aattttcgct
    19861 ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg gagtttggtc cggcaaaacg
    19921 ctggccacac taccactatg cggagctggc aaagcaactg attgatgaag gttatcaggt
    19981 ggttctgttt ggctcggcga aagatcatga agcgggcaat gagattcttg ccgctttgaa
    20041 taccgagcag caggcatggt gtcgtaacct ggcgggggaa acacagcttg atcaagcggt
    20101 tatcctgatt gcagcctgta aagccattgt tactaacgat tctggcctga tgcacgttgc
    20161 ggcggcgctc aatcgtccgc tggttgccct gtatggtccg agtagcccgg acttcacacc
    20221 gccgctatcc cataaagcgc gcgtgatccg cctgattacc ggctatcaca aagtgcgtaa
    20281 aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc agcgcgtact
    20341 ggaagaactc aacgcgctat tgttacaaga ggaagcctga cggatgcggg ttttgatcgt
    20401 taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg atgcccagca
    20461 ggcaatccca gggattaagt ttgactgggt ggtggaagaa gggttcgcac agattccttc
    20521 ctggcacgcc gccgttgagc gagttattcc tgtggcaata cgtcgctggc gtaaagcctg
    20581 gttctcggcc cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac aagcagagaa
    20641 ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcgctgg tgacgcgtct
    20701 ggcgcatggc gtaaagcatg gcatggactg gcaaaccgct cgcgaacctt tagccagcct
    20761 gttttacaat cgtaagcatc atattgcaaa acagcagcac gccgtagaac gcacccgcga
    20821 actgtttgcc aaaagtctgg gctacagcaa accgcaaacc cagggcgatt atgctatcgc
    20881 acagcatttc ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc
    20941 gacgacccgt gatgataaac actggccgga agaacactgg cgagaattga ttggtttact
    21001 ggctgattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc
    21061 gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga gtctggaagg
    21121 cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca
    21181 tttaacggcg gcactggata gacccaatat cacggtttat ggacctaccg atccgggatt
    21241 aattggtggg tatgggaaga atcagatggt ttgtagggct ccggggaatg agttgtctca
    21301 attgacagca aatgctgtta agcaattcat tgaagaaaac gctgaaaaag ctgccatgat
    21361 ttaaattaca actctttaat agataatttt gaaataaaat cagcttcctt gctgatttt
//
LOCUS       DAZKJR010000087        21323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKJR010000087 DAZKJR010000000
VERSION     DAZKJR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.087"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..831
                     /locus_tag="ACHOWS_003272"
     CDS             <1..831
                     /locus_tag="ACHOWS_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFQ0048804.1"
                     /translation="FVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTRKLESIFNNTQV
                     QIAVLIIPTTKDETIEQYATRVFNNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGLEE
                     KVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYSANPSESESASSS
                     DHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDLFLFSDKTFSIAVF
                     SFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSGGGFSGGGGSSGGGGASGRW
                     "
     gene            complement(891..2189)
                     /gene="eno"
                     /locus_tag="ACHOWS_003273"
     CDS             complement(891..2189)
                     /gene="eno"
                     /locus_tag="ACHOWS_003273"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0048805.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2277..3914)
                     /gene="pyrG"
                     /locus_tag="ACHOWS_003274"
     CDS             complement(2277..3914)
                     /gene="pyrG"
                     /locus_tag="ACHOWS_003274"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFQ0048806.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4142..4933)
                     /gene="mazG"
                     /locus_tag="ACHOWS_003275"
     CDS             complement(4142..4933)
                     /gene="mazG"
                     /locus_tag="ACHOWS_003275"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFQ0048807.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEQIKTEERAQKAQHSALDDIPSSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQVVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5029..7263)
                     /gene="relA"
                     /locus_tag="ACHOWS_003276"
     CDS             complement(5029..7263)
                     /gene="relA"
                     /locus_tag="ACHOWS_003276"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFQ0048808.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7311..8612)
                     /gene="rlmD"
                     /locus_tag="ACHOWS_003277"
     CDS             complement(7311..8612)
                     /gene="rlmD"
                     /locus_tag="ACHOWS_003277"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0048809.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8669..11425
                     /gene="barA"
                     /locus_tag="ACHOWS_003278"
     CDS             8669..11425
                     /gene="barA"
                     /locus_tag="ACHOWS_003278"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFQ0048810.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11649..12989)
                     /gene="gudD"
                     /locus_tag="ACHOWS_003279"
     CDS             complement(11649..12989)
                     /gene="gudD"
                     /locus_tag="ACHOWS_003279"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFQ0048811.1"
                     /translation="MSSQFSTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13010..14350)
                     /locus_tag="ACHOWS_003280"
     CDS             complement(13010..14350)
                     /locus_tag="ACHOWS_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFQ0048812.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVGLDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14352..15704)
                     /gene="gudP"
                     /locus_tag="ACHOWS_003281"
     CDS             complement(14352..15704)
                     /gene="gudP"
                     /locus_tag="ACHOWS_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFQ0048813.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWEQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16139..16588)
                     /locus_tag="ACHOWS_003282"
     CDS             complement(16139..16588)
                     /locus_tag="ACHOWS_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0048814.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     SGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16606..17388)
                     /gene="truC"
                     /locus_tag="ACHOWS_003283"
     CDS             complement(16606..17388)
                     /gene="truC"
                     /locus_tag="ACHOWS_003283"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFQ0048815.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMLVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17388..17717)
                     /locus_tag="ACHOWS_003284"
     CDS             complement(17388..17717)
                     /locus_tag="ACHOWS_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFQ0048816.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDKPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18339..18884)
                     /gene="syd"
                     /locus_tag="ACHOWS_003285"
     CDS             complement(18339..18884)
                     /gene="syd"
                     /locus_tag="ACHOWS_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFQ0048817.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18952..19800
                     /gene="queF"
                     /locus_tag="ACHOWS_003286"
     CDS             18952..19800
                     /gene="queF"
                     /locus_tag="ACHOWS_003286"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFQ0048818.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19912..21276
                     /gene="ppnN"
                     /locus_tag="ACHOWS_003287"
     CDS             19912..21276
                     /gene="ppnN"
                     /locus_tag="ACHOWS_003287"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFQ0048819.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5225 a         5695 c         5115 g         5288 t
ORIGIN      
        1 tttgttgcag cccaaccaac tattgtccca caattacagc aacaagttac ggatcttacg
       61 agtagcttaa actcacaaga aaagaaagaa ctgacgcgga agttagaatc tattttcaat
      121 aatacccaag tacaaattgc tgtattaatt attcccacaa ccaaagacga gacaattgaa
      181 caatatgcta caagggtttt taacaattgg cgtttaggag atgccaaacg caatgatggg
      241 atactgatca ttgttgcctg gtcggatcgc actgtccgca tccaggtagg ttatgggctg
      301 gaagaaaagg taaccgatgc tctggctgga gatatcatcc gtagcaacat gatacccgca
      361 tttaaacaac aaaaattagc taagggatta gagttagcta taatcgcttt gaataaccaa
      421 ctcacttcac aacaccaata ttcggctaat ccttcagaaa gtgaatcagc gtcttccagc
      481 gatcattact actttgctat tttttgggta tttgcagtga tgttcttccc tttctggttt
      541 tttcatcaag gcagtaattt ttgtcgcgca tgtaaaagca gtgtctgtat ttcagcaatc
      601 tatcttttag atttattcct gttctccgat aaaacttttt ccattgctgt attttccttc
      661 ttttttactt tcaccatatt tatggtcttt acctgtttat gtgtacttca gaaaagagca
      721 tcaggtagaa gttatcattc agacaacagc ggttccgctg gaggctcagg cggcgggttt
      781 agcggcggtg gcggttcttc tggcggtggc ggcgcatccg gccgctggta actcaaccat
      841 aaaaaacgcc agcccgaagg ctggcatttt taaatcagat aaagtcaggc ttatgcctgg
      901 cctttgatct ctttacgacc gttgtacggt gctttttcgc ccagagcttc ttcgatacga
      961 atcagctggt tgtatttagc aacacggtca gaacggctca tagaaccagt tttgatctgg
     1021 cctgcagcag taccaacagc caggtcagcg atagtagcgt cttcagtttc gccagaacgg
     1081 tgagagataa ctgcagtgta gccagcgtct ttcgccatct tgattgcagc cagagtttcg
     1141 gtcagagaac cgatctggtt gaatttgatc aggatggagt tagcgatacc tttttcgata
     1201 ccttctttca ggatcttggt gttggttacg aacaggtcgt caccaaccag ctggattttg
     1261 tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt cagattcgtc cagaccgtct
     1321 tcgatagata cgatcggata ctgtttggtc agttcttcca ggaagtgagt gaattcttca
     1381 gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt taccgtcttt gtagaattca
     1441 gaagctgcgc agtccatcgc cagagtgatg tctttgccca gttcataacc agcagcttta
     1501 acagcttcag cgataacagc cagagcttca gcgttggaac ccaggttcgg cgcatagcca
     1561 ccttcgtcac caactgcagt gttcatgcct ttcgctttca gaacttttgc caggtgatgg
     1621 aaaacttcag aacccatgcg gatggcttct ttcacagttt tcgcgccaac cggctgaatc
     1681 atgaattcct ggatatcaac gttgttgtca gcgtgctcac caccgttgat gatgttcatc
     1741 atcggaaccg gcatggagta tttgcccgga gtgccgttca gttcagcgat gtgctcgtac
     1801 agcggcatac ctttagcagc tgcagcagct ttggcgttag ccagagatac agccaggatt
     1861 gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca ggtcgatcat gatcttgtca
     1921 atgccagcct gatctttagc atctttgcca atcagcgcct gagcgatcgg gccgtttacc
     1981 gcagcaacag ctttggttac gcctttaccc aggaaacggg atttgtcgcc atcgcgcagt
     2041 tccagagctt cacgggaacc agtagaagca cctgacggag cagctgccat accgacgaaa
     2101 ccaccctcca gatgtacttc ggcttcaaca gtcgggttac cacgggagtc gatgatttca
     2161 cgaccgatga tttttacgat tttggacatt aggttttcct caagtcacta gttaaactga
     2221 aactccagac aaacaacgcg tacccagggt acgcgttgcc gttctaactt ttttacttac
     2281 ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa agcctgcaaa cagcgggtga
     2341 ccatcacgtg gagtagaagt aaactccgga tggaactggc aagccacgaa ccacgggtga
     2401 ttcggaactt cgatgatctc gaccaactga tcatccccgg aacggcccgc aacgcgcaga
     2461 cctgcatctt caatctgttt caacagcatg ttgttgactt cgtaacggtg acgatgacgc
     2521 tcaacaattg tcggcgcatt gtacagctgg cgaaccaggc tatcgtcaac caactggcac
     2581 tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct tctcgctacg aacttcaacg
     2641 ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa ctgggtactt acagtctggc
     2701 acaaattccg tagagttggc gttctccatg ttggcaacat ggcgagcgta atcaattaac
     2761 gccacctgca tacccaggca aatacccaga taaggaatat tgttctcacg cgcaaaacgc
     2821 gcggtcgtaa tcatgccttc tacgccacga tagccgaaac cgccaggtac gaggattgcg
     2881 tccagacctt taaggatttc aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg
     2941 atgttgacgc tgacacgatt cttcagccca ccgtgtttca gtgcttcgat cactgattta
     3001 taagcatccg gcagttcaat gtacttgccg accataccga tggtgacttc acttaccgga
     3061 ttcgcttctt cgaagataac ctgttcccat tcggacagat ttgcttccgg gcagtttaag
     3121 ctgaatcgtt tacaaatata atcgtccagc ccctgagatt tcaacaggcc cggaatttta
     3181 tagatggaat cgacgtcttt cagagaaata accgcttttt ccggaacatt acagaacaat
     3241 gcaatcttcg cacgttcgtt cgccggaacg gcgcgatctg aacgacaaat caggatgtca
     3301 ggctggatac cgatggagag cagctctttt acagagtgct gagtcggttt ggttttgact
     3361 tcaccagacg ctgccatgta cggcaccagc gtcaggtgca taaacagagt gtgctcacgg
     3421 ccaatttcaa cagccatctg gcgaatcgct tcgaggaacg gcagggattc gatatcacct
     3481 acagtaccgc cgatttcgac cagtactacg tcatgacctt cgccgccttc cagcacgcgc
     3541 tctttgattg cgttagtgat gtgcggaata acctgcacgg ttgcgccgag gtagtcaccg
     3601 cggcgttctt tacgcagaac gtcagagtag atacgacccg tggtgaagtt gttgcggcgg
     3661 ctcattttgg tacgaatgaa acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg
     3721 tcttcagtaa cgaacacttc cccgtgttgg attgggctca tagtacctgg atcgacgttg
     3781 atgtacggat ccagtttcat gatggtcaca ttgaggccac gggcttcaag aatggctgcg
     3841 agggaggctg cggcaatgcc tttacccaga gaggatacga ccccgccggt cacaaaaata
     3901 tagttcgttg tcatgctgaa cctgagaagt taggttgaaa agacgatgga ataaccagga
     3961 cgggaaagca gtatacccga acatgacctg tgccacaaac tttcattatc cctcctcttc
     4021 gccagcgcac tattgaaatc aggagtgaga aaatagcccc tttggggtaa atgtttttga
     4081 cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatc gcgcttgacc acgtaattcc
     4141 cttagagatc aatttcctgc cgttttacct gttgccagac ttcttccatt gtttcgaggt
     4201 caacacctgt catttccagt ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac
     4261 gctcgaattt ttcgttcgct ttttgcaatg cgatttccgc ttttgtccct aaatgacgag
     4321 ccagattgac tgtggcaaac agcaggtcac ccatttcctc ctccagttta gcctggtcga
     4381 caacaacctg ccgcgcttcg tacatcacct catcgatctc ttcgtagact ttatcgacta
     4441 ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca acgtttctgg attttttgtg
     4501 cgcgcattaa ggcgggtaaa ctactaggga tatcgtccag cgccgaatgc tgcgcttttt
     4561 gcgcgcgctc ttcagttttg atttgctccc aacgagcaag aacttcgcgg ctgtttccgg
     4621 cagaaccgtc agcaaaaaca tgtggatgac ggcgttctaa tttatcgcta atcgcagcgc
     4681 aaatatcatt aaaatcaaag cgcccttctt cctgggccat ttgtgcgtaa aacaccacct
     4741 ggaacagcag atcgcccagt tcaccgcgca gatcgtcaaa atcttcacgg gcgatggcgt
     4801 ccagcacctc gtaggtttct tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt
     4861 tatcccacgg gcagccgttt tccgggtcac gcaggcgctg catgatggtg agcaaacggt
     4921 cgatttgatt cattagaatg tcctgaaaat tgcgggtccg tcaggtgtaa taccttcgag
     4981 taaaagatat cgctggatac ggctgacgcc atatccagct atgtaagact aactcccgtg
     5041 caaccgacgc gcgtcgataa catccggcac ctggttgagt ttacccagca cgcgccccag
     5101 cacttgcagg ttgtaaatct caatggtcat gtcgatggtc gccagttgct gtttggtgtc
     5161 gctacggctg gcaacgccaa gtacgttcac cttctcgttg gcgagaatgg tcgtgatatc
     5221 acgtaacaac ccactacgat cattagccac cacgcggacc accagcgaat atccggcgga
     5281 gtagctctcg ccccataccg cgtcaacaat gcgttctggc gcatgggagc gcagttccgc
     5341 caattgttcg caatcggcgc ggtgtactga aataccgcgc ccctgggtaa tgaagccgac
     5401 aatctcatct ccaggaatcg gctggcagca gcgcgcgatg tggtgcatca ggttaccgac
     5461 cccttctacc accacacgac cgttatcttt actgcggttt tgcggcgtgt agcttttttg
     5521 ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca ctcggcttat taaattgcga
     5581 ttgcaggaag ttcaccatct gattgagacg gatatccccg ccaccaatcg ccgccagcaa
     5641 ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt ttttctgctt ctttcaggct
     5701 gatccccaga tgttccagct cgtcgtcaag aatttgccgc ccagccagaa tgtttttgtc
     5761 acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc ccacggctgg ttgtgacgta
     5821 accgaggttt gggtttaacc agtcacggct ggggttcggc tgtttctggg tgataatttc
     5881 aatctggtcg cccatctgca gctggtaggt gaacggcaca atgcgcccgc caatttttgc
     5941 cccgatgcag cggtgtccga catcactgtg gatgtggtaa gcgaagtcca gcggcgttga
     6001 tcccgcaggc aaatcaacga catcaccttt cggcgtaaag acgtacactc ggtcgtcaaa
     6061 gacctgacta cgtacttcgt cgagcatttc gccggaatca gccatctctt cctgccacgc
     6121 aatcagttta cgcagccagg caatccggtc ttcatgtccc gaacgtgcgc cgccagcagc
     6181 cgcgccctct ttatatttcc agtgcgcagc aacacccaac tctgcatctt catgcatctg
     6241 tttggtgcgg atttggatct caacggtttt gccacccggc cccagaacca cggtatgaat
     6301 ggactgataa ccgtttggtt tcgggttagc gacgtaatcg tcaaactcat ccggcaggtg
     6361 gcgatagtga gtgtgcacta tccccagtgc ggcataacaa tcctgtaaac gctcggcgac
     6421 aatacgtacc gcacgcacat caaacagctc atcgaaggcg aggttctttt tctgcatttt
     6481 gcgccagata ctgtagatgt gtttcggacg gccatacact tcagctttaa cgccttcagc
     6541 tttcatctca gcgcgcagat ggccgacaaa ctcttcgata tagtgttcgc ggtcgagacg
     6601 gcgttcatgc agcagttttg cgatgcgttt gtattcggtc gggtggagat aacggaagca
     6661 gtaatcttcc agctcccatt tcagttgccc aatcccaaga cggttcgcca acggcgcgta
     6721 gatattggtg cactcttttg ccgccagtac gcgttcatct tccggcgcat cttttacttc
     6781 gcgcaggtga gcaatacgct ccgccagttt gatgaccacg cagcgaaaat catcgaccat
     6841 cgccaataac atccggcgaa cgttatcgac ctgttcggag gaaacagaat cagtgtgcgt
     6901 cgctttcagc tggcggatcg ccgccatatc acgcacgccg tgaataaggt taacgaccga
     6961 cttaccgacg ctctcacgca gcacatcttc gctgactacg ttggcatcag ccagagggaa
     7021 cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat gtcgagagga tctccaccat
     7081 ctcaacacca cgccacaata acagactggc atccggatgc ccctgcgtct gttgcagaca
     7141 atacgcccag gtttcggcta agcactcaca cgacttctgg ctggtaatac ccagacttgc
     7201 gatccatttt tccggatcaa attcaccagc cttattgata tgtgcacttc ttaccgcaac
     7261 catcgtcctc tcctttaggg accagacctg ccgaaatcgg caaatcgtaa ctatttaacg
     7321 cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg ggaacatatc cagcatcgcc
     7381 agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc tatcccgagc cagcgttgca
     7441 gggttacagg atacataaac tatacgaata ggttccagtt ttataatttg ctgcataaca
     7501 cctgcggcac ctgctcgcgc cgggtccaac aacactttat cgaagccgtt tttcgcccac
     7561 ggctgctttg tgacatcttc ttcaagattt tcgtgataaa acgtcacatt ctgtaagcca
     7621 ttaagacgcg cattctgctg gcctttttcc accagcgccg gaacaccttc tacacccacc
     7681 acactagcag cttgtgtcgc caatggcagt gtaaagttgc ccataccgca gaacagatcc
     7741 agtacgcgat cttcaggttc cacatccagc cattccaacg cacgcgctac cattttctgg
     7801 ttcacacccg cattgacctg aataaaatcg cgcgggctaa aagttaagcg caacccattt
     7861 gagtcatacc agggcatctc accagagacg gtttcgagta tctcactatc gggggcgaga
     7921 tacagatcca ggccttcaga atgcgaaaag cgttccagtt tttcgcgatc agccgaactt
     7981 agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg ttgcctgtac cagttcaaca
     8041 tgaccaagat ggcgtatagc ttgtaagctg cccaggcatg ccctgacttt gggcagcaat
     8101 gcttcaagtt ggggcactaa aatgggacat tgtttaacgt cgacaatgtc actggagccc
     8161 gctttgcgaa accccatctg aagttgctgt gttttcggta agtagttcag acttaaacgc
     8221 gcgcgacggc gatagcccca gggaacatcg gcgatcactt cagagacttc gtgtttcatt
     8281 aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat ccacgctggc gtgttgttgc
     8341 tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg tttcgcgttc cgggctatcg
     8401 cttaaccggc gcacgacttt agcgcgggcg tattgttttt tatcttcagt aacagtaact
     8461 tccgcgtttt cctgtggcaa taatccagga ataaataacg ctttgccgtt atgtcgtgcc
     8521 acgccctgac caaaagagtc gaggtcgttg actgaaacag ttatgatctg acgcgtcgtc
     8581 gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga ctttctcaat ttaacagtgt
     8641 gaccttaatt gtcccataac ggaactccat gaccaactac agcctgcgcg cacgcatgat
     8701 gattctgatc ctggcaccga ccgtccttat tggtttattg ctgagtatct ttttcgttgt
     8761 gcatcgctat aacgacttgc agcgtcaact ggaagatgcc ggagccagca ttattgagcc
     8821 gcttgcagtt tctactgaat atggcatgag cctgcaaaat cgcgaatcta tcggtcagtt
     8881 aataagcgta ctgcatcgtc gccattccga tattgttcgc gcgatttcgg tttatgatga
     8941 aaataaccga ctctttgtca cctccaattt tcatcttgat ccctcatcaa tgcagctcgg
     9001 cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt gacggcgata ttatgatcct
     9061 ccgcacgccg attatttctg aaagttactc ccccgacgaa tcgcccagta gcgatgccaa
     9121 aaatagtcaa aatatgctgg gatatattgc gctggagctg gatcttaaat cggttcgctt
     9181 gcagcaatat aaagagatct ttatttccag cgtgatgatg ctgttttgta tcggtattgc
     9241 gcttattttt ggctggcgct taatgcgcga tgtaaccggt ccgattcgca acatggtgaa
     9301 taccgtcgac cgcatccgtc gcgggcaact cgacagccgg gtggaaggat ttatgctcgg
     9361 cgagctggat atgctgaaaa acggtatcaa ctcgatggca atgtcgctgg cggcttatca
     9421 cgaagagatg caacacaata tcgaccaggc gacgtccgat ctgcgtgaga cgctggagca
     9481 aatggagatt cagaacgttg agttggatct ggcgaaaaag cgcgcccagg aagcggcgcg
     9541 tattaaatcc gagtttctgg caaatatgtc acacgagctg cgtacaccac tgaatggtgt
     9601 tattggcttt acccgcctga cgctgaaaac agaattaaca ccaacgcagc gcgatcacct
     9661 gaatacgatt gaacgttcgg caaataattt gctggcaatt attaatgatg ttctcgactt
     9721 ctcgaaactg gaagccggta agctgattct ggaaagtatt ccattcccac tacgcagcac
     9781 gctggatgaa gtcgttaccc tgctggcaca ttcttctcac gataaagggc tggaactgac
     9841 gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc gacccactac gattacagca
     9901 aatcatcact aacctggtag ggaatgcaat taaattcacc gagaatggca acatcgatat
     9961 tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag attgaagtgc agattcggga
    10021 taccggcatt ggtattcctg aacgcgatca atcgcgctta ttccaggcct tcagacaggc
    10081 tgatgccagt atttcccgcc gtcatggtgg caccgggctg gggctggtga ttacacaaaa
    10141 actggttaat gaaatgggcg gcgatatttc gttccatagc ctgccgaatc gtggttcaac
    10201 tttctggttc cacattaatc tcgatctgaa cccgaacatt attatcgaag ggccatccac
    10261 ccagtgcctc gctggtaagc gcctggccta tgtcgaacca aactccgcag cagcacaatg
    10321 cacgctggat attttaagtg aaacgccgct ggaagtggtt tatagcccaa cgttctccgc
    10381 gctgcctccc gcgcattacg acatgatgtt attaggcata gcggtgacct tccgcgagcc
    10441 gctaacaatg caacatgagc gattagcgaa agcagtatcg atgaccgatt tcctgatgct
    10501 ggcacttcct tgccatgcac aagtcaatgc tgaaaaactt aagcaagatg gtatcggcgc
    10561 gtgtctgctg aaaccattaa cacctacgcg cctgttgcct gccctgacgg aattttgtca
    10621 tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt aagctggcaa tgacagtcat
    10681 ggcggttgat gacaaccccg ctaacctgaa acttatcggc gcattgctgg aagatatggt
    10741 gcaacatgtg gaactttgcg atagcgggca tcaggcggtt gaacgggcga aacagatgcc
    10801 gttcgattta atcttaatgg atattcaaat gccggacatg gacggcattc gggcatgtga
    10861 gctcatccac cagctcccgc atcagcagca aacgccggtt atcgcggtaa cggcgcatgc
    10921 gatggccggg caaaaagaga aactgcttgg cgcggggatg agtgattatc tggccaaacc
    10981 gatcgaagaa gagcgattgc ataatttgct gttgcgctac aagcctggca gcggtatttc
    11041 ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtc aacccgaatg cgaccctcga
    11101 ctggcaactg gcactacgcc aggcagcagg aaaaaccgat ttagcgcgcg atatgctgca
    11161 aatgttactc gatttcctgc ctgaagttcg caacaaagtt gaggaacagc tggttggaga
    11221 aaacccggaa ggcctggttg atttgattca taaactgcat ggcagttgcg gctatagcgg
    11281 tgtaccgcgt atgaagaatc tctgccaact gatcgaacaa cagctacgta gcggtactaa
    11341 agaagaagat ttagagccgg agctgctgga actgttggac gagatggata atgtagcgcg
    11401 cgaagccagc aaaattctcg ggtaatggac gtcttgtcga gtagcaaccg gatttttatg
    11461 acgctggttt cagattggtg aaaagtgcct gtgtttatgc cggatgcggc gttaacgcct
    11521 tatccggcct acaaaaccaa ttaaattcaa tgaattaaaa aatgatgtaa ccctgatacg
    11581 tagcgcaaca ggcagtttgg catcagtctc aaaccggctc cagatagagc cggttttggt
    11641 tttctgtctt aacgcaccat gcacgggcgc ttgttatcaa acgtccagcc ggggatcagg
    11701 tactgcattc ccatcgcatc gtctcgcgca ccaagcccat gtttctgata cagctcgtgg
    11761 gctttcatca cctgatccat atcgatttct acgcccaatc ctggtttttg cggcacctgt
    11821 accagcccgc ctttgatctc aaacggttct ttggtcagac gctgattgcc ttcctgccag
    11881 atccagtgcg tatcaatagc agtaatttta cccggtgcag cagcggctac atgagtaaac
    11941 atcgccaggg aaatatcgaa gtggttgtta gagtgtgaac cccaggtcag gccaaattca
    12001 tggcacattt gcgccacacg taccgaacct tgcatcgtcc agaaatgcgg atccgccagc
    12061 gggatatcaa cggattgtaa cgagagcgta tggcccatct gccgccagtc ggtggcgatc
    12121 atattggttg cggtcggcag gccagtcgcg cgacggaact ctgccatcac ttcacgcccg
    12181 gagaaacctt gctccgcacc acacggatct tctgcataag ccagcgaacc tttcaggtat
    12241 ttaccaattt taatcgcttc gttcagcgac caggcaccgt taggatcgag ggtaatacgc
    12301 gcctgtggga agcgttgcgc cagtgccaca atagattctg cttcttcttc cccggccagt
    12361 acaccgccct tcagtttgaa atcgttgaag ccatattttt catatgccgc ttccgccagg
    12421 cgcaccaccg catccggcgt catcgcttct tcgtgacgca ggcgatacca gtcacattta
    12481 tcatccggct ggctttgata cggcagcggt gtggctttgc gattaccgac gaagaacaga
    12541 taaccgagca tttcgacttc gctacgctgt tgaccatcgc ccagcagcga tgccacgtta
    12601 acccccagat gctgccccag cagatccagc attgccgctt ctatcccggt aaccacatga
    12661 atagtggtcc gcaggtcaaa tgtctgcaaa ccgcgcccgc cggcatcacg atcggcaaat
    12721 gtattacgca ccagcgtcag aacgtttttg tattcaccca gcgttttacc caccaccagc
    12781 ggaatcgcat cttccagcgt tttacggatt ttctcgccgc cgggaatttc ccctaccccg
    12841 gtgtgaccag aattatcttt gataatcaca atattacgcg tgaagaacgg tgcgtgtgca
    12901 ccactcagat tcatcagcat actgtcatgg cccgccaccg ggatgacctg catttcagta
    12961 acaacaggcg tcgaaaattg agaactcatc tttatgtcct ttttcaaaat caatgacggc
    13021 cgaaaacggg acgtttacgg tcaaaggtcc agccggggat caggtactgc atcgggcccg
    13081 cgtcgttacg cgcaccccca ggcagacgtt tataggcctc atgcgccttt tgtacctgct
    13141 cccagtccag tcccacgccc agaccgggcg catcaggaac ggcaattttt ccgtttttaa
    13201 tctccagcgg atttttggtc aggcgacaat cgccctcctg ccaaatccag tgggtatcga
    13261 tagcggtagg attacccggt gccgccgcac ccacatgggt aaacatcgcc agagagatat
    13321 caaagtggtt attggaatgg cagccccagg tcagccccca gtcgtcgcaa agttgcgcca
    13381 cgcggactgc accggaaagc gtccagaagt gcggatcggc aagtggaata tctaccgcat
    13441 tgagcatcac cgcatgaccc atttcgcgcc agttggtggc gatcatgttg gtcgcgacgg
    13501 gcaagccggt cgcccgtcga aactccgcca tcacttcacg accggagaaa ccctgctcag
    13561 cgccgcacgg gtcttccgca taggtaagga catcattcag ccctttgcac agagaaatgg
    13621 cttcatcaag cagccaggca ccattgggat caacggtaat ccgcgcatca gggaagcgtt
    13681 tcttcaatgc acgaacagtg tcgatttctt gctcgccagg taacacgcca cccttaagtt
    13741 tgaaatcttt aaagccatag cgatcctgtg aggcttccgc cagacgcaca acggcttcgc
    13801 tgttcatcgc tttctgatgc cgcaactgat accactcatg gttgcccggc gtattttcca
    13861 gataaggaag atcggttttg gtccgatcac cgatataaaa cagataaccg agaacggtaa
    13921 tagcatcacg ttgcttgcct ggccctaaca gttcgcagac cggaacattc agcgcctgac
    13981 ctaacaggtc aagcaaggcg gcttccagcg ccgccacggc attaacgcgc aattcaaaag
    14041 tccaggcacc tttgccgaag gtatcaaaat cggctgcctg attaccttta tgcacctgct
    14101 gaaccacttt attcaggcgc gcaacttcct ggcccagcac catcggaata gccttgacca
    14161 gtgtttgata aatcacctct ccgcccggcg cttcaccaat gccggtatgc ccggcattat
    14221 cggtgagtac cacaatattg cgggtgaaat atgcgttatg tgcaccacca atattaagca
    14281 acatgctgtc atgtccggcg accggaatga ctttcatatc agtaataaca ggactggatt
    14341 gtgtcgtcat cttattgccc cgcgacaggt ttcagctcaa tacgcttgat atcgcccacc
    14401 agcaccaggt agctgagcac cgcgattaag gcatgaacac caacataaat cagcgcccca
    14461 ttaaaggagc cagtcgtgcc aacgatataa ccaattgcga ttggcgtgac gatgccagaa
    14521 atgttaccaa acatgttaaa caggccaccg gaaagaccgc tgatctcttt tggcgcagta
    14581 tcagccatta cagcccaacc cagcgcaccg atccccttac cgaagaaggc cagcgccata
    14641 aagccgatga tcatccactc aacgttgacg tagttgcaga acaccatcac catcgacaac
    14701 aacatgccca tcacgatcgg tgttttacgc gcaatgttca gtgaacccgt gcgacgcatc
    14761 agccagtcgg aaataatccc acccagcaca ccgccgataa aaccgcaaac cgccggaacg
    14821 gaagccacaa agcccgcttt cagaatcgac atcccgcgcg cctgcaccag ataaaccggg
    14881 aaccaggtaa tgaaaaagta agtcagggcg ttgatacagt actgaccgat ataaacgccg
    14941 atcatcatcc gtgacccgag cagctgtttg atctgctccc acttcacgct gaacggcact
    15001 ttaactttgg tgttttgctg atccatattg atcagcgcgc cacccgcggc gatgtactcc
    15061 agctctttct gatttacgcc cggatgttga tttggctcgt ggatgacttt caaccagata
    15121 aagctgatga caatgcccag accgcccata aagaagaaga cgtgtgacca gcccacttca
    15181 tgagttagcc agcccataat cggcgcgaag ataaccgttg cgaagtattg ggcggagtta
    15241 aaaatcgaca ccgccgttcc cctttcctgc gccggaaacc aggccgcaac aatgcggctg
    15301 ttgccaggga aagatggcgc ttcagcaagc ccgaccagga agcgcagtgt aaacagggca
    15361 acgataatgc cgaatccact aaagatatcg acgaagcctt gcagcaaggt aaacatcgac
    15421 cagataaaga tcgaccagaa gtagacgcgt tttgaaccaa aacgatccag caaccagcca
    15481 ccagggatct gcccgataac atacgcccat gagaaggcag agaacacata gcccattccc
    15541 acgggatcaa ggccgatatc tttggccatt tccgaaccgg caatagaaag cgtagcgcgg
    15601 tcgccgtagt tgaaggatgt gacgataaac aacatcacca ctatccagta acgagcattc
    15661 gtgcgttttt ccacactgct cgcagcctga cttaaagaac tcattgttgc actcctgaaa
    15721 attcgcgtta gccacgctca ctctggactg cgacatcgcc aggaaatcag aggtgacgta
    15781 gggtgttttt tgccgttttt ataggtcgtt cgccgaatac ggcgcgtgtt tatatctggc
    15841 aatagcagta taaaaagcgc gccatagcgg ctcaccgtgc aacaacacaa cattaatgcg
    15901 ttcaatgagg cccgattttg gcattagccc tggacggtgg aatccacttc acggaaatga
    15961 aaacaagaac aagaaaggaa gggttaaaac gaagaaataa agaagagtat gaaatggatc
    16021 gcttgactcc aggcaaacgc cagtaaaaat ccgcgctatg aagcagtttt tactggcatt
    16081 tgcctgaaaa gattcgattc agcaccgcta aaacggcatt taccgctcgc tgaacatatc
    16141 aggacaacat cgtgccccaa tgttcgaccc acggatttga ttccgtttcc ggttccgggt
    16201 tctcactggc gtcaatcaac agcatttcgc caacccgctg agcgctctgt tcctgcaaca
    16261 aggcatcgaa ctgtttgcca ctattgcaga aattcacata actactgtcg ccgagcgcaa
    16321 tcacgccata acgcagattc ggctggaagc ccagggtatc tttaattccc tgaaagagcg
    16381 gcacaatgct atcaggaagg tcgccctgcc cggtcgtgga cgtaaccacc agaacatact
    16441 tatcctgata gggcagccag tcgcttaatt caggatcttc aaataccgtt gctttgtggc
    16501 cctgcgcggt cagaattgct tccgcttctt ccgccactaa cagcgaattt ccgtacatag
    16561 tgccgacaaa aataccaatt tccgccatac cgttttctcc ctggattagg aacttatctc
    16621 tccatcctga cccgatggcg cactgaactc aaccctttca ttttcaggaa gcagaccccg
    16681 ccagccaaat tgtgataacg cctgcatcca ggtgtcgtcc aggcccgcgt gaatagtcag
    16741 tggctcgcca gtaaaaggat gcgtcaatga caactgactg gcatgcagca ttaaccgctg
    16801 aaggccaaaa tgttcagcac cgctgcgatt ctggcgtaaa tcaccatgtt tgctgtcgcc
    16861 aataatcggg tggcgcaaat gggcaagatg gcgacggagc tggtgcttac gtccggtttt
    16921 cggctccagt tccaccaggc cgtagcgcgt ggtcgggtaa cgtccggtcg ctaccagcat
    16981 ttctacggtc gccagaccgc gataatgcgt cactgctggc tgcgggcctt tatcttcgcg
    17041 ggcaaattta tcagcgattt tgtccagttc ttccaccagt ggataatcca gcaccgcttc
    17101 ttccatcaac cagccgcgca caatcgcatg gtaacgtttc tggatttggt gctgttcaaa
    17161 ctgttgtgcc agcagccgtc cggcctcgct ggataatccc atcaacaaca caccagaagt
    17221 gggtcggtcc agacgatgag cagtaaacac atgctggcct atcaggtcac gcacggtttg
    17281 catgaccact actttctcgt cgcgatccag ccagctgcgg tgaaccagcc agccggaggg
    17341 tttatttacc gcaaccagcc attcatcctg atagagtatt tccagcatta gctcgcatca
    17401 tccgcaaaaa gagcatccag tttttccagc tcagccagaa taagcgcgcg ttgcggatga
    17461 tcagtcgcca gcgccatttc ataatagggt gcaacggcaa aagcgcccgg taacggctgg
    17521 ttgttatcta acaaatcgtg cattcgcggg atcagcaccc actgcaacca ctccagtggt
    17581 tccatggtgt ccataaagaa cggttgggta ctgttaaatt gatgcggctg gggtttatcg
    17641 tttcgccagt gctgatgttc acgcagtaac gcttcaagcg cctgcaactg gagacgaacg
    17701 cggtcatgag tggtcataaa gcaacctcaa taagaaaaac tgccgcgaag gatagcagga
    17761 ataaaaaaag ggagcactgt attcacagtg ctcccggttc gtttcgcagc attccggcta
    17821 cttttgttgc tccctgctca tccttgacaa cttttcctct ggccttgcgg ccaatcgttc
    17881 atcctgaact attgcttcct gctcacacca ccccgatgtg atacttcatc ctgaagtgtt
    17941 cctggccttc ctgacccacc gaatcatcct gaccggttct cattctccat cctggaggtg
    18001 tcctttaacg cgtcctgcgt catcctcttc gcttcatcca gaagcctttc cctgaaacac
    18061 catcctggtg tgtccttcag aagtgtcatc atcctgatgt tcacttcgtt gtctgactcc
    18121 ctgtcgacga agatagaatc gtctttttcg cgaagtctta caaggcgctt acagacaatg
    18181 ccttaaggaa aatttcatat gaaagttaag aattaacaag caacatgttg tttttaaatg
    18241 agaataacga ttcttatctt tttaagtatt cgcttatttc ctggcgatct cctacagagt
    18301 ctgtaagaga tctctcacaa ccagatgggg tatttggatt acagaagagg cttaagttga
    18361 ttaaggaatt ccgcaagatt tgaggctaaa tgcgtccgtt tacgtgttcc tagcgtctct
    18421 ttgcacactt caccggagag attacacacc gagatgacct ctaactcttc ttccagcgtc
    18481 gcgataaata gcgtgggcgg cagttttagc cttttctgag tcaccagatg accaatcaga
    18541 ttttcctgca cccgacggaa atcgtcttca ctccaggttt gcagcaatgt cagtttgata
    18601 tcgccaaact gcgcgtgcat atccccggca aactgagtcg tataaaaggt atgaatcgtg
    18661 ggttgtatca caatatcaaa agcgcgttca accgcgttta cattttgttc cccggtaaac
    18721 ggctggggtt gccagtatac ggcatcttcg gtagttgaaa taatgcacgg tgaaggaacg
    18781 ccgtacagtt cctcacttaa cggccagctt ttatgctctt catgccacgc atcgcagtag
    18841 cgtgcagtaa aatctttcag ggcttgtgcg gtcaaatcgt ccactaattt ctctcttcac
    18901 ttaaaccaga tacacttgtc gtttagttta tctggtttat gacggtgaaa catgtcttct
    18961 tatgcaaacc atcaggcact tgcgggcctg actcttggaa aatcaaccga ttaccgggat
    19021 acctatgacg ccagcctact gcaaggcgtt ccacgcagcc tgaatcgcga cccgctgggt
    19081 ctgaaagcgg ataacctgcc ttttcacggt acggatatct ggacgctgta tgaactttcc
    19141 tggctgaatg cgaaaggttt gccgcaggtc gctgtcggtc atgttgaact tgattacacc
    19201 agcgcaaatc tgattgagtc gaagagtttt aagctctatc tcaacagttt taaccagacg
    19261 cgttttaata actgggatga ggtgcgccag acgctggagc gcgacttaag cacttgcgct
    19321 cagggtaagg ttagcgtggc gttatatcgt cttgatgaac tggaaggcca accgataggt
    19381 catttcaatg gcacttgcat tgatgaccag gatatcacta tcgataacta tgaattcact
    19441 actgactatc tggagaatgc caccagtggc gaaaaagtag tagaagagac gctcgtcagc
    19501 cacctgctga aatcaaattg cctgatcacc catcaaccag attggggatc gatccaaatt
    19561 cagtatcgtg gacgccaaat tgacagagaa aaactgctgc gttacctggt ttctttccgt
    19621 catcacaacg agttccacga acagtgcgtg gaacgcatct ttaatgacct gttacgcttc
    19681 tgccagccag agaaattgag cgtttacgca cgttataccc gtcgtggcgg tctggacatt
    19741 aacccgtggc gcagtaatag cgattttgtc ccatcgacca caagactggt tcggcaataa
    19801 attttttctc aattttgcgt gctggattca cgcagaaggt tgtgaaaggt catcaggcag
    19861 ggctattgta atcaaaggga atgacgatat tcgtcccata aggagttttt cttgattaca
    19921 catattagcc cgcttggctc catggatatg ttgtcgcagc tggaagtgga tatgcttaaa
    19981 cgcaccgcca gcagcgacct ctatcaactg tttcgcaact gttcacttgc cgtactgaac
    20041 tccggtagtt tgaccgataa cagcaaagaa ttgctgtctc gttttgaaaa tttcgatatt
    20101 aacgtcctgc gccgtgaacg cggcgtaaag ctggaactga taaatccccc ggaagaggct
    20161 tttgtcgatg ggcgaattat tcgcgctttg caggccaact tgttcgcggt tctgcgtgac
    20221 attctcttcg tttacgggca aatccataac accgttcgtt ttcccaacct gaatctcgac
    20281 aactccgtcc acatcactaa cctggtcttt tccatcttgc gtaacgctcg cgcactgcat
    20341 gtcggtgaag cgccaaatat ggtggtctgc tggggcgggc actcaattaa cgaaaacgag
    20401 tatttgtatg cccgtcgcgt cgggaatcag ctgggcctgc gtgagctgaa tatctgcacc
    20461 ggctgtggtc cgggagcgat ggaagcgccg atgaaaggcg ctgcggtcgg acacgcacag
    20521 cagcgttaca aagacagtcg ttttattggt atgacagagc cgtcgattat cgccgctgaa
    20581 ccgcctaacc cgctggtcaa cgaattgatc atcatgccgg atatcgaaaa acgtctggaa
    20641 gcgtttgtcc gtatcgctca cggtatcatt atcttccctg gcggtgtggg tacggcagaa
    20701 gagttgctgt atttgctggg aattttaatg aacccggcca acaaagatca ggttttacca
    20761 ttgatcctca ccggcccgaa agagagcgcc gactacttcc gcgtactgga cgagtttgtc
    20821 gtgcatacat tgggtgaaaa cgcgcgccgc cattaccgca tcatcattga tgacgccgct
    20881 gaagtcgctc gtcagatgaa aaaatcgatg ccgctggtga aagaaaatcg ccgcgacaca
    20941 ggcgatgcct acagctttaa ctggtcaatg cgcattgcgc cagatttgca aatgccgttt
    21001 gagccgtctc acgagaatat ggctaatctg aagctttacc cggatcaacc cgttgaagtg
    21061 ctggctgccg acctgcgccg tgcgttctcc ggtattgtgg cgggtaacgt aaaagaagtc
    21121 ggtattcgcg ccattgaaga gtttggtcct tataaaatca acggcgataa agagattatg
    21181 cgtcgtatgg atgacctgct acagggtttt gttgcccagc atcgtatgaa gttgccaggc
    21241 tcagcctaca tcccttgcta cgaaatctgc acgtaatctc cctcccccgg tgacgtttgt
    21301 tgccgggttt tctcgttttt ggt
//
LOCUS       DAZKJR010000088        21282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKJR010000088 DAZKJR010000000
VERSION     DAZKJR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.088"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..2260
                     /gene="fadB"
                     /locus_tag="ACHOWS_003288"
     CDS             71..2260
                     /gene="fadB"
                     /locus_tag="ACHOWS_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFQ0048820.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKMA"
     gene            2270..3433
                     /gene="fadA"
                     /locus_tag="ACHOWS_003289"
     CDS             2270..3433
                     /gene="fadA"
                     /locus_tag="ACHOWS_003289"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFQ0048821.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMIGDAQTCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLTQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISSTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(3625..4326)
                     /gene="fre"
                     /locus_tag="ACHOWS_003290"
     CDS             complement(3625..4326)
                     /gene="fre"
                     /locus_tag="ACHOWS_003290"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFQ0048822.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(4372..5865)
                     /gene="ubiD"
                     /locus_tag="ACHOWS_003291"
     CDS             complement(4372..5865)
                     /gene="ubiD"
                     /locus_tag="ACHOWS_003291"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFQ0048823.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            6032..6520
                     /gene="rfaH"
                     /locus_tag="ACHOWS_003292"
     CDS             6032..6520
                     /gene="rfaH"
                     /locus_tag="ACHOWS_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFQ0048824.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(6517..7299)
                     /gene="tatD"
                     /locus_tag="ACHOWS_003293"
     CDS             complement(6517..7299)
                     /gene="tatD"
                     /locus_tag="ACHOWS_003293"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFQ0048825.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKVPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(7341..8117)
                     /gene="tatC"
                     /locus_tag="ACHOWS_003294"
     CDS             complement(7341..8117)
                     /gene="tatC"
                     /locus_tag="ACHOWS_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFQ0048826.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(8120..8635)
                     /gene="tatB"
                     /locus_tag="ACHOWS_003295"
     CDS             complement(8120..8635)
                     /gene="tatB"
                     /locus_tag="ACHOWS_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFQ0048827.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(8639..8908)
                     /gene="tatA"
                     /locus_tag="ACHOWS_003296"
     CDS             complement(8639..8908)
                     /gene="tatA"
                     /locus_tag="ACHOWS_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFQ0048828.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(8987..10627)
                     /gene="ubiB"
                     /locus_tag="ACHOWS_003297"
     CDS             complement(8987..10627)
                     /gene="ubiB"
                     /locus_tag="ACHOWS_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFQ0048829.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(10624..11229)
                     /gene="ubiJ"
                     /locus_tag="ACHOWS_003298"
     CDS             complement(10624..11229)
                     /gene="ubiJ"
                     /locus_tag="ACHOWS_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFQ0048830.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(11243..11998)
                     /gene="ubiE"
                     /locus_tag="ACHOWS_003299"
     CDS             complement(11243..11998)
                     /gene="ubiE"
                     /locus_tag="ACHOWS_003299"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFQ0048831.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(12093..13520)
                     /gene="rmuC"
                     /locus_tag="ACHOWS_003300"
     CDS             complement(12093..13520)
                     /gene="rmuC"
                     /locus_tag="ACHOWS_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFQ0048832.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            13737..14504
                     /locus_tag="ACHOWS_003301"
     CDS             13737..14504
                     /locus_tag="ACHOWS_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0048833.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            14528..15532
                     /locus_tag="ACHOWS_003302"
     CDS             14528..15532
                     /locus_tag="ACHOWS_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFQ0048834.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            15522..16151
                     /locus_tag="ACHOWS_003303"
     CDS             15522..16151
                     /locus_tag="ACHOWS_003303"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFQ0048835.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            16176..17150
                     /locus_tag="ACHOWS_003304"
     CDS             16176..17150
                     /locus_tag="ACHOWS_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFQ0048836.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMAYSKANPGKVRIGN
                     SGTGAIWHLAVAALEDKTGTKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            17167..17673
                     /locus_tag="ACHOWS_003305"
     CDS             17167..17673
                     /locus_tag="ACHOWS_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFQ0048837.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            17686..19176
                     /locus_tag="ACHOWS_003306"
     CDS             17686..19176
                     /locus_tag="ACHOWS_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFQ0048838.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(19210..19974)
                     /gene="udp"
                     /locus_tag="ACHOWS_003307"
     CDS             complement(19210..19974)
                     /gene="udp"
                     /locus_tag="ACHOWS_003307"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFQ0048839.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            20236..21048
                     /locus_tag="ACHOWS_003308"
     CDS             20236..21048
                     /locus_tag="ACHOWS_003308"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFQ0048840.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
BASE COUNT         5054 a         5711 c         5472 g         5045 t
ORIGIN      
        1 ttttaacaca aaatacacac ttcgatgcat ctggtacgac cagatcacct tgcggattca
       61 ggagactgac atgctttaca aaggcgacac cctgtacctt gactggctgg aagatggcat
      121 tgccgaactg gtatttgatg ccccaggttc agttaataaa ctcgacactg ccaccgtcgc
      181 cagcctcggc gaggccatcg gcgtgctgga acagcaatca gatctaaaag ggctgctgct
      241 gcgttcgaac aaagcagcct ttatcgtcgg tgctgatatc accgaatttt tgtccctgtt
      301 cctcgttcct gaagaacagt taagtcagtg gctgcacttt gccaatagcg tgtttaatcg
      361 cctggaagat ctcccggtgc cgaccattgc tgccgtcaac ggctatgcgc tgggcggtgg
      421 ctgcgaatgc gtgctggcga ccgattatcg tctggcgacg ccggatctgc gcatcggtct
      481 gccggaaacc aaactgggca tcatgccggg ctttggcggt tctgtacgta tgccacgtat
      541 gctgggcgct gacagtgcgc tggaaatcat tgccgccggt aaagatgtcg gcgcggatca
      601 ggcgctgaaa atcggtctgg tggatggtgt agtcaaagca gaaaaactgg ttgaaggggc
      661 gatggcgatt ttacgccagg ccattaacgg cgacctcgac tggaaagcga aacgtcagcc
      721 gaagctggaa ccgctaaaac tgagcaagat tgaagccacc atgagtttca ccatcgctaa
      781 agggatggtc gcacaaacgg cggggaaaca ttatccggcc cccatcaccg cagtaaaaac
      841 cattgaagcg gcggcccgtt ttggtcgtga agaagcatta aatctggaaa acaaaagttt
      901 tgtcccgctg gcgcatacca acgaagctcg cgcactggtc ggcattttcc ttaacgatca
      961 atatgtaaaa ggcaaagcga agaaactcac caaagacgtt gaaaccccga aacaggccgc
     1021 tgtgctgggc gcaggcatta tgggtggcgg catcgcttac cagtctgcgt ggaaaggcgt
     1081 accggttgtc atgaaagata tcaacgacaa gtcgttaacc ctcggcatga ccgaagccgc
     1141 gaaactgctg aacaagcagc ttgagcgcgg caagatcgat ggtctgaaac tggctggcgt
     1201 gatctccaca atccacccaa cgctcgatta ctctggtttt gaccgcgtgg atgttgtggt
     1261 agaagcggtt gtcgaaaacc cgaaagtgaa aaaagccgta ctggcagaaa ccgagcagaa
     1321 agtacgcccg gataccgtgc tggcgtctaa cacttcaacc attcctatca gcgaactggc
     1381 caacgcgctg gaacgtccgg aaaacttctg cgggatgcac ttctttaacc cggtgcaccg
     1441 gatgccgttg gtggaaatta ttcgcggcga gaaaagctcc gacgaaacca tcgcgaaagt
     1501 cgtcgcatgg gcgagcaaga tgggcaagac gccgatcgtg gttaacgact gccccggctt
     1561 cttcgtcaac cgtgtactgt tcccctattt cgccggtttc agccagttgc tgcgcgacgg
     1621 cgcggatttc cgcaagatcg acaaagtgat ggaaaaacag tttggctggc cgatgggtcc
     1681 ggcatatctg ctggatgttg tgggcattga caccgcgcat cacgcccagg ctgtcatggc
     1741 agcaggcttc ccgcagcgga tgcagaaaga ttatcgcgat gccatcgacg cgctgtttaa
     1801 tgccaaccgc tttggtcaga agaacggcct cggtttctgg cgttataaag aagacagcaa
     1861 aggtaagccg aagaaagaag aagatgccgt cgttgacgac ctgctggcag aagtgagcca
     1921 gccgaagcgc gatttcagcg aagaagagat tatcgcccgc atgatgatcc cgatggtcaa
     1981 cgaagtggtg cgctgtctgg aagaaggcat tatcgccact ccggcagaag cggatatggc
     2041 gctggtctac ggcctgggct tccctccgtt tcacggcggc gcgttccgct ggctggacac
     2101 cctcggtagc gcaaaatacc tcgatatggc acagcaatat cagcatctcg gcccgctgta
     2161 tgaagtgccg gaaggtctgc gtaataaagc gcgtcataac gaaccgtact atcccccggt
     2221 tgagccagcc cgtccggttg gcgacctgaa aatggcttaa ggagtcacaa tggaacaggt
     2281 tgtcattgtc gatgcaattc gcaccccgat gggccgttcg aagggcggtg cttttcgtca
     2341 cgtgcgtgcg gaagatctct ccgctcattt aatgcgtagc ctgctggcgc gtaacccggc
     2401 gctggaagcg gcggccctcg acgatattta ctggggttgt gtacagcaga cgctggagca
     2461 gggttttaat atcgcccgta acgcggcgct gttggcagaa gtgccgcact ctgtcccggc
     2521 ggttactgtc aatcgcttgt gtggttcatc catgcaggca ctgcatgacg cagcacgaat
     2581 gatcatgatc ggcgatgcgc agacatgtct ggttggcggc gtggagcata tgggccatgt
     2641 gccaatgagt cacggcgtcg attttcaccc cggccttagc cgtaatgtcg ccaaagcggc
     2701 gggcatgatg ggcttaacgg cagaaatgct ggcgcgtatg cacggtatca gccgtgaaat
     2761 gcaggatgcc tttgccgcgc ggtcacacgc ccgcgcctgg gccgccacgc agtcgggtgc
     2821 atttaaaaat gaaattatcc cgaccggtgg tcacgatgcc gacggcgtcc tgacgcagtt
     2881 taattacgac gaagtgattc gcccggaaac caccgtggaa gccctcgcca cgctgcgtcc
     2941 ggcgtttgat cccgtcagcg gtacggtaac ggcgggtaca tcttctgcgc tttccgatgg
     3001 cgcagctgcc atgctggtga tgagtgaaag ccgcgcccat gaattaggtc ttaagccgcg
     3061 cgcccgtgtg cgttcgatgg cggtcgttgg ttgtgatccg tcgattatgg gttacggtcc
     3121 ggttccggcc tcaaaattgg cgctgaaaaa agcggggctt tctgtcagtg atatcggcgt
     3181 gtttgaaatg aatgaagcct ttgccgcgca gatcctgcca tgtattaaag atctgggact
     3241 gattgagcag attgacgaga agatcaacct caacggtggc gcgatcgcgc tgggtcatcc
     3301 gttgggctgt tccggtgcgc gtatcagcag cacgctgcta aatctcatgg agcgcaagga
     3361 tgcccagttt ggtcttgcga cgatgtgtat cggtctgggt cagggtattg cgacggtgtt
     3421 tgagcggatt taaccacttg ccagatgcgg cgtgaacgcc ttatccggtc taccgatcct
     3481 gcaccaattg taggcctgat aagacgcgcc agcgtcgcat caggcagcgt gtgccgaatg
     3541 ccggatgcga catagatcta aatgccactg tataaagttt tagttgccgt tcttcccgcc
     3601 tgtcaggggc gggtttttta tatctcagat aaatgcaaac gcatcgccaa acaggcgatc
     3661 ttcccgcgca ttacgctcgc tgcaaaacag gtcacgggca attttcgcca tctcaaaacg
     3721 tccggcaata tagatatcat gctctgccag cgtaccatga tcctgcaata ccgccgttaa
     3781 cacggtgcca gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg
     3841 tagaccagga tgcttcaacg aaagcgcctc aagctcgcag agatcataca gatgctgctc
     3901 ttcacgcccg ccccagtaaa tggtgatatc acggtttggg ttacgcgcca acgctgtcag
     3961 caaaatcgag cgggcataag agaacccggt gccgcccgca atcaaaatca tcggacgctc
     4021 ttcatcatcg cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt
     4081 gaggatgcgg tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag
     4141 ctcgataaac cctttttcat ctggcgtcga agccattgag aacggacgtt tgtcgcgctc
     4201 atccatcact accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggcacgat
     4261 gcggacacga tatacggtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt
     4321 tgtcatgcgc tttctctgtc ggatcgataa atagggcaaa acaaacgcgc atcaggcgct
     4381 tttaccgttg ttaaaaatag ccagttcatc ccaaatggcg tcaatatgcg cgacaacatc
     4441 tggatctttt ttgatgggac gtccccattc acgctgggtt tcccccggcc atttattcgt
     4501 ggcatccagc cccatttttg aacccagccc ggagacaggg gaggcaaaat ccagataatc
     4561 aataggcgta ttttctacca gaacggtatc ccgcgccggg tccatacggg tggtaatcgc
     4621 ccaaatcaca tcgttccagt cgcgggcgtt gacgtcatca tcgcaaacga tcacaaattt
     4681 agtgtacata aactggcgta agaacgacca gacgcccatc atgacgcgct tcgcgtgtcc
     4741 ggcatactgt tttttgatcg tcactaccgc cagacgataa gaacagcctt ccggcggcag
     4801 ataaaaatcg acaatttccg ggaactgttt ttgcagaatc ggcacgaaca cttcgttcag
     4861 tgctacaccc agtaccgcag gttcatctgg cggacgcccg gtataggtgg aatgataaat
     4921 cgcatcttca cgctgggtaa tatgcgtcac ggtaaatacc ggaaaactat cgacttcgtt
     4981 atagtaaccg gtgtggtcgc catacggccc ttccggcgca gtttcgcctt gttcgatata
     5041 cccttccagc acaatctccg cactggcggg cacttcaagg tcattggaga tacacttcac
     5101 cacttcggtc ttggtgccgc gcagcaatcc ggcaaacgca tactctgaaa gcgtatccgg
     5161 aacgggggtg actgcaccga gaatcgtggc gggatcggca cccagcgcca cagaaaccgg
     5221 gaaacgttcg cccggatgcg ccgcacacca ctcctgataa tccaacgcgc cgccgcgatg
     5281 cgacagccag cgcataatca gtttgttttt accaattagc tgctggcgat aaatgcccag
     5341 attctgccgc tctttatgcg ggccgcgcgt tacggtgagc ccccaggtaa tcagcggcgc
     5401 ggcatcttcc ggccagcagg tcataatggg aatacgattg agatcgacgt catcgccaga
     5461 gacgattttt tgttggcaag gcgcaccacg cagccgcttt gtcggcatgt tcaatacttg
     5521 cttaaactgc ggcagtttat caaacaggtc gcggaaacct tttggcggct ccggctcttt
     5581 cagaaacgcc aataatttac caacttcacg cagcgccgaa acatcttcct gccccatgcc
     5641 catcgccacg cgctttggcg taccgaacag gttgcacagc accggcattg agtagccttt
     5701 agggttttcg aacaacagcg caggcccacc ggcacgcaaa gtgcggtcag caatttcagt
     5761 gatttccagg tgcggatcca ccgggagcgt gatacgtttt agctcaccct gctgttcaag
     5821 cagcgtcaag aagtcgcgta aatcgttata tttcatggcg tccattgtag cctcttaatc
     5881 tgcgcccatt atacggcgtt catctttgcg atgctgtaaa tttgttaaat tagcgtgaac
     5941 tctgacggta taacgcaaac cggggaatat aattaactta gcgtaaagct tttgctatcc
     6001 ttgcgccccg attaaacgga taagagtcat tatgcaatcc tggtatttac tgtactgcaa
     6061 gcgcgggcaa cttcaacgtg cccaggaaca cctcgaaaga caggctgtga attgcctggc
     6121 accgatgatc accctggaaa aaatcgtgcg tggcaaacgt actgcagtca gtgagccatt
     6181 gttccccaac tacttgtttg tggagttcga tccggaagtg attcatacca cgactatcaa
     6241 cgcgacccgc ggtgtcagcc acttcgtgcg ctttggcgcg tcgccagcga tagtcccatc
     6301 ggcggtgatt catcagttat cagtgtataa accgaaagat attgtcgatc catcgacgcc
     6361 gtatcctggt gataaagtga ttattactga aggcgcattc gaaggttttc aggccatttt
     6421 caccgagcca gacggcgagg ctcgctccat gctattgctg aatcttatta ataaagagat
     6481 taagcacagt gtgaagaaca ccgagttccg caaactctaa aacgcaatcc caaacagtgt
     6541 tttgacatta gcatccgtgg tggcagccag ccatgcggca tcttctccac gccagtgcgc
     6601 aatacgttgc aaaatatggg gcagatgggc tggctcgttg cgccgggatg atggctttgg
     6661 cgtgagatcg cgagggagca gatacggcgc atcagtttcg atcagcaatt tctccgccgg
     6721 aatcaacggc aacaattccc gcagctccag cccgcgtcgt tcatcgcaaa cccaaccggt
     6781 aatgccgata taaattccat gcgccacgca cgcctgcatc tcttcgcgtg tgccggtaaa
     6841 gcaatgaaga accgcaccag gcactttatc cagccacggc tccagcaatg tcataaaccg
     6901 ctcgtgggca tcgcgacagt gcataaatac cggcatgttt aattctgcgg caatgcgtag
     6961 ctgggcaaca aaagcgcgtt cctgttcttc cggcgtcgaa aagttgcggt taaagtcgag
     7021 accacattca ccaatcgcca ccacttctgg ctgcgcggcc agctcaataa tcgcttcttc
     7081 agtcgcagct tgccactggc tgctgtcgtg aggatgtacg cccgccgttg accaacagga
     7141 cgaatactga cgcgccagct tttgcgcctg ctggctttca cgcaggttag tgccggtgat
     7201 gagtagccca ttaactcccg cgtcaaaagc gcgcgctaca acatcatcac ggtctttcgc
     7261 aaattgcgaa ctggtcaaat taacgccgat atcaaacatc ctgtactcca tatgacaacc
     7321 gccctgacgg gcggttgaat ttattcttca gttttttcgc tttctgcttc agcgtcgttt
     7381 tcctcttccc gatttcgccc tttaccaacg taaaagcgtg agaagaagac accgatttca
     7441 aacaggcaat acatcgggat cgccagcagc gtttgcgaga agacatccgg cggcgtcagc
     7501 agcatcccga caacaaatgc gccaaccagc acatacggac gttttttgcg taagtcttct
     7561 ggcgaggtaa ttcccatcca gcacagcagc actattgcca ccggcacttc aaaggagaca
     7621 ccaaacgcca taaacagcgc cataacgaag cttaaatagc tggcgatgtc ggtggatacc
     7681 tgtacccctt ccggcgcggt attggcaagg aagccaaatg ccagcggaaa gaccacaaag
     7741 taggcgaacg ccatgccgat ataaaacaac agagagctgg aaaccagcag cggcaccacc
     7801 aggcgacgtt catgcttata cagcgctggg gcgataaacg cccacacctg atagagaatc
     7861 accggcgctg acagaatcag cgacaccata aaggtcagct tgatcggcgt aaagaacggc
     7921 gaggccacgt cggtggcgat catcgttgaa ccttgcggca actgcttgat caatggcgcg
     7981 gataccaggt gatagatgtc attggcgaaa tagaccagac acaggaatat cacgatcacc
     8041 gcgataatgc agttcagcag acgcttacgc agctcaatca gatgcgtgat aagcggttga
     8101 gtatcttcta cagacatgtt tacggtttat cactcgacga aggggaaggt gcagcggttt
     8161 tcggttcagc gtccgcagca ggttttacca ccggctctgg cgtggtttct ggcttctgtt
     8221 ccggcgaact ggcttgcgtt tgtgcagcag caggcgttac gccttcatgc gcagcttcat
     8281 tgtctttcac caccgggttg tggatggtgt gcgcttcatc gctcgccttt tcaggatcgt
     8341 ttgcaacgta ggaacgcttc atcgactccg cggcctggcg tagttcatcc atcgacgctt
     8401 tcagttcggg cgtcaggtta gtgaggctcg ccttttcaac ctttttcaga ctgtcctgaa
     8461 actcctggag ttttaactcc tgggtcagtt cgttctgcac cgttgtcgcc agtgaacgca
     8521 acgcgcgaat ccagcccgcg accgttttta ccgccacagg cagtcgttgc ggccccagaa
     8581 cgacgaggcc gatgatgaat accaatagca gttcgctaaa accgatatca aacacggatt
     8641 acacctgctc tttatcgtgg cgcttcgcgt cttctgtttt agcctgttcc tgattcgtat
     8701 ccgcctgctt atcggcgata gttttcgcag taaaatcagc atcctgactg gttttatcct
     8761 gctttggttc atcatcgctc attgcttttt taaagccttt gatcgacgca ccaagatcgg
     8821 aaccgatgga gccgagcttt ttggtgccaa aaagcagtac aacgatgacg gcaataatca
     8881 ataactgcca aatactgata ccacccatac atgttcctct gtggtagatg atgattaaac
     8941 aaggccgcat tatacgttac acggcccgcc ttgagcgatg aaaaaatcag cgtgttttgc
     9001 gccaaccgac aaaccaggcg atcagaccac ctgccattaa ccagccgggc atcagccccc
     9061 attcaggtcg gctgaccaac aagaatgtgc cacttaatac taacgtagcg ccaattccga
     9121 gaaaataacg cgattgtccc tgacgtacat gatttgactg aagctcgcgg gcaatcttat
     9181 caacactgtg ctgtaaatac ttgccctggc gcaaactgtc gtaaaccagt tcaggcagtt
     9241 ctggcatttt ttcgacccag aacggcgctt tttctttaaa tgctctcacc agcgcaggaa
     9301 taccgacctg atctttaatc cacgactcaa ggaaaggctt cgccgttttc cataaatcga
     9361 gctgcggata aagctggcgt cctacccctt cgacgtagag cagggttttc tggagtaaca
     9421 ccagttgcgg ctgcacttcc atattgaagc gacgcgccgt attaaataga tttaacagca
     9481 catgtccaaa cgaaatttcg gccaacggtt tctcaaagat aggttcacag accgtacgaa
     9541 tggcaaattc gaactcttca acgttggtat ctggcggcac ccagcctgaa tcgacgtgta
     9601 gctctgcaac tttgcgataa tcgcgattaa agaaagcgat aaagttttcc gccagatagc
     9661 gtttatcttc tttgtttagc gagccaacaa tcccgcaatc aatgccgata tatttcgggt
     9721 tttctgggtg ttcatagctt acaaagatgt tgccagggtg catatcggca tggaaaaagc
     9781 tgtcgcgaaa gacctgagtg aagaacacct gcacgccgcg ttccgccagc aatttcatgt
     9841 tggtgccgtt tttctccagc gtcgcaacat cagacaccgg aatgccgtaa atacgctcca
     9901 tcaccatcat cccttcacta caatagtcag ggtaaacttc cgggatgtag agcatcgggc
     9961 tgtcttcaaa attgcgtcga agctgaatgg cgttggcaga ttcccgcagc aaattcagtt
    10021 catcaatcaa ggtcttttcg tactcgcgca ccacttcggt tgggcgcaga cggcgaccat
    10081 ccggcagcaa acgcggcacc cagcgagcca gacggtagat aagtttcaga tccgctttaa
    10141 tgaccggcaa aatatccggg cggatgactt taatcaccac ctctttacca ttcgatttca
    10201 atcgcgcggt atgaacctgg gcgatagaag cagaagccag cggcttgatt tcaaaatcgt
    10261 caaaccacgc ttctaccggc aagccgccca ttgcagcttc aatctgctgc ttcgccagct
    10321 tgccatcaaa cggagcgact ttgtcctgca ataacgccag ctgatcggca atatgcggcg
    10381 gaaaaagatc gcggcgggtt gataacatct gcccgaactt gatccatacc ggtcccagtt
    10441 cttgcagggc cagtcgtagt cgctcaccta aaggtttgtc tttatgccga tttggcatcc
    10501 agaataatga gtatcgccat agccgtagcg gcagggtgat acgcattttg gggatcagtt
    10561 catcaagtcc gtagcttaaa aaagtgcgaa tgatgaaata taggcgccgt acttcacctg
    10621 gcgtcattta gcctccagtt tttccagccg tttggtcagg gcatcaacag cacgctcgac
    10681 ggcagccgtc tcttccgcaa accaggccac ttcaagcgga ccgggtgcca tacgccactc
    10741 ttcagtaatg gcttccgcca cataacgttg ctggcgctta atgccgtgat gcaggaactt
    10801 tgcgcccccg cgcagggctt tgctgatacc ttcagcggcg atatcaccgg tataaggggc
    10861 cagcagttcc gcagggtcga actcagccag atctgccagc gcaacgaagt tttgcaccac
    10921 ctgaatatcg ccctgcactt ccagctcacc actgcgaatc agtgcggcaa gctgctggcg
    10981 atcgcgaagt ttcggcaaca cactggcgta ggcgataacg gtgcagtcag catcgcctgc
    11041 ccattcgccc agtacatcaa cctggcgttc gctgaacacc agaatcaatg acgtcgaaaa
    11101 gccttttacc tccacgcgca gcactttgcc caacagacgc gagcgggccg ttttcagcgc
    11161 gggtgagcga tacaggaagg tgttgagcag actttcaatt cctgccgtca ctaaaggttt
    11221 aaaaggcatt tccggtctcc tgtcagaact tataaccacg atgcagcgcc acaacccctg
    11281 ccgtcagatt gtagtagtcg acactttcga atccggcatc ctgcatcatg gttttcaggg
    11341 tatcctgatc gggatgcata cggatggatt ctgccagata acggtagctg tcggcgtcgt
    11401 tcgcgaccag tgagccaata cgcggcagca catggaagga gtatgcatca taggctttgc
    11461 tcagcggctc gataattggc ttcgagaact caagcaccag caggcggccg ccgggtttca
    11521 gcacgcgata cattgaacgc agtgctttat ctttgtcggt gacgttacgc agaccaaacg
    11581 aaatggtgat gcaatcaaag gtgttatccg ggaacggcag cgcctcagcg ttcgcctgaa
    11641 catactcaac gttgccaatc acaccgatat tacgcagctt ctcgcggccc attttgagca
    11701 tggattcatt gatatcagca aggaccactt tgccagtttc tccgaccagg cgagagaatt
    11761 tcgctgtcag gtcgccggtg ccaccagcca gatccagcac ggtctgccca cggcgtacgc
    11821 cgctgcaatc aatcgtgaat cgcttccaca aacgatgaat accgaatgac atcaaatcat
    11881 tcatgacatc gtattttgat gccacggaat ggaaaacgtg ggcgaccata tccgcttttt
    11941 gttccttcgc gacggtctga aaaccaaagt gcgtcgtttc ttgtgactta tccaccatct
    12001 caatgcctgc tcatcaaaaa attgttccag aagtgtaaca gattgggcgt cgatgcccta
    12061 gatttctacc cggcttaact acccccaatg ggctagcgcg actgctgatt atattcatca
    12121 tcgcgttgat aagcttcatc attcggctgc tccggaaccg accgaagtcg atactcttca
    12181 tcctggctca ccgcctgttc agccaaatcc ggattaatct cgcgtttaat ttctacccct
    12241 aaaccgcgaa acgcttctgc ctgcgccagc acatttccgc gacctgaaga gagttttttc
    12301 attgcctggc ggtagttatc ctgcgctttg tcgagacttt gaccaatcgc tgacatgtca
    12361 tcgacgaaca aacgcatctt gtcgtacagc ttgctggcac gatcggcgat ttgctgggcg
    12421 ttgcggcttt gatgctcata acgccacagg ttggcgatag tgcgcagcgc caccagcagc
    12481 gtagtcgggc taaccagcat gatattgttt ttcaacgctt cggtgatcag ctccggctgg
    12541 cggtcaagcg ccagtaaaaa agcgggttca acgggaataa acatcagcac gtaatccagg
    12601 gttcgcagcc ccggcagctg ttgataatct ttgcgtccca gcaaacggat atggttacgc
    12661 accgacgcga tatgttcctg tagcgcgctt tcgcgggtgt agtcatcttc ggcgttgaaa
    12721 tagcgttcat aggcgaccag cgtcattttg gcgtcgatca ccacatcttt tccctgcggc
    12781 aaccgcacga tgacatccgg ctgcatccgc gagcgggcgt cattttcgat gctgacctga
    12841 gtttcatatt catacccttc acgcagaccg gaagcctcca gcacccgcgt caataccacc
    12901 tcgccccagt tgccctgggt tttattgtcg cctttcagcg cgcgcgtcag gttgatcgcc
    12961 tcctgagcca tttgcgcgtt gagctgctgg agattgcgaa tttcgtgggt cagggtatga
    13021 cgttcctgtg cttctttacc gaagctgtcc tgaacctgac ggcggaaacc gtccagttgt
    13081 tcacgtagcg gcgacaacag gctgttcaga ctctgacggt tttgctcatc aacccggcga
    13141 ttgctgtgct caaaaatacg gttggcgagg ttttcaaact gctcactgag gcgctgctcg
    13201 ctgttgatca tctggcgaat tttatcgtca gcatgttgct gtgcggcttc catccgcgtg
    13261 gttacttcac gcagatcggc ctccagagag gtgttaatac tttgcaggct gcgcacttcg
    13321 ttattgagta actcgcactc agcgcgccag tgctcgcttt gagtaatttg ttgttttgcc
    13381 gcgcttaact ccgcgaccat ctcttcacgt tcagctaatt gctcggcttt ttgctgcgca
    13441 tgttgataac tggcaaacag ccagccaatg accacactaa ccagcgcaat aatggcataa
    13501 acaatgattg agaaatccac aaagcctcct gacccaacaa cttacccgca caaaatagaa
    13561 ttgtgctgta caaacgtcca gtttgaaaga agtttcctgc gaggcgctac gcaaaacgat
    13621 cgttaatttt cattatttta tcccacaata tgagatggag tttatattgt gggatatatc
    13681 accttttccc cacgtcatta tgtggaacac ttcagcaata tcatcaggag aaacgtatgg
    13741 cagaaacctc ttcaaccacc gcaggcgcac aaacgttatt acgtggactg gcggttctta
    13801 atgctgttta taacggctgc catgacctaa aaagtatcgg cgagttcacc ggcacgaccc
    13861 ggagcacaac ccatcgttta gtcaccgttc tggtggaaca acgctacctg cgccatgtcc
    13921 caacgcaggg ctaccaactg ggtgccaaac tgatcgaatt cggtgccaga gcactggaaa
    13981 gcacctcact gtacgaaatt gcccagcccg ttttgcaacg ccttgcccgt tacaccctgg
    14041 acaccgtgca tctggggatc gttgaaggcg acgaagtgct gtatctggag aagatcaaca
    14101 gtcagcgcgg gctggaaatg cgctcacgcc caggccatcg catgccactg gcaatcaccg
    14161 gcatcggcaa ggcattgatc ctcaaccgaa cagaagaaga gtggcgcacg ctgtttaaga
    14221 cctgtggtga tgaaacaaaa ctgggcgcat ttattcagaa catgcgtcgt tatgccgcca
    14281 gtggttttgc ttttgatctg gaagagaacg agccgactat ccgttgcgtt gcagcgccgg
    14341 tttacaacgc ccgggatgaa attgtggcgg ctatttctgt cgccagtacc accacgtata
    14401 tgtcgctggc gcgtctggaa gaactggcac cttacgtcaa atcctgtgca gaagagatat
    14461 ccgctgaact gggctggggt aagcacgtcc gcaaagataa ataaaccatg attaaaggaa
    14521 acgactaatg ccgggagcac cctcatttat tgccctggac tggggaacgt catcgcttcg
    14581 ggcatggcgt tttggcgaat caccaaatcc acaagaaaaa cgtgaattcc cctgggggat
    14641 catgaaatta ccctcccagg cagcaacacg tgaggacgct ttccacgata cgtttttacg
    14701 tgtctgcggc gactggctgg cacaaacccc atgcccggta ctggcttgcg gcatgctcgg
    14761 cagtgcgcaa gggtggcaat cagcagctta tttaccttgc ccggtcactc tcgagggtct
    14821 ggcgaagcag ttgacgcctg ttatccacca gcagcagaca atgctgcaca tcattcctgg
    14881 cgtgattaaa gaaggtgaaa tgcccgaagt gatgcgtggc gaagagacac aaatcttcgg
    14941 tgctatctcg atggaaccgg ccctgcaaaa cgcgatccat cagggtatgc cagtgctgat
    15001 aggcttaccc ggcacacatg cgaaatgggc agtagttgaa aacaacacca ttaccgattt
    15061 ccgaaccttt atgacgggcg agttatttga tgttttatcc cgccattcga ttctcggtgc
    15121 caccatgcat cctggagatg aaccgcactg ggatgccttc acccacgggc tgacagcagc
    15181 acaagagcat catcagaccg gattattatc gacgctgttt tcgacccgtt cgcgcctgct
    15241 gaccagtaat cttacgtcgt cctcgcaggg ggattacctt tccggattac tgataggcca
    15301 tgaattatgt ggtctggcat ccagtttgct acgtgattta cccgccacaa caccgatcgc
    15361 cttaatcggt agcgcaaatc tgaacagccg ctattcacaa gcattcagcc acgtttttcc
    15421 cgacagacag atacacgcca ttcctaatgc cactgaacat ggattatggc gaatcgccca
    15481 cgctgccggg ttactgtcca ccaacgccag ggaatgtacc catgccattt aatacacttt
    15541 tgcaaaaaac cgggttagtc gccattttac gcggcgtgaa accggatgag attgtcgcca
    15601 tcggtgaaaa actgtacgcc gccggattcc gcctgattga gattcctatg aactcgccgg
    15661 aggcgttgca aagtatcagt ttactgcgcg acgccctgcc taaggactgc ctggtcggag
    15721 ccggaactgt gctttcggta gagcaggtgg ttgcggttaa agaggctggg ggccagatta
    15781 tcgtgatgcc gcattgcgat actgcggtga tccgtcgcgc ccgtgcgctg ggtatgtatt
    15841 gtgcgcctgg cgtcgccacg cccaccgaag cctttgctgc cattgaacat ggtgctaatg
    15901 ccatcaagct gttcccggct gaacaaatta cgccagaagt taccaaagcc tggcgtgcgg
    15961 ttattccaca atccgttccg atgttaccgg ttggcggtat cacaccggaa accatggcgc
    16021 gttatctgag ccacggcgca aacggctttg gcctcggttc tgcgctatat cgccccggca
    16081 tgacgccaga gcaagtctat gaaaatgcag tactgtttat gaacgcatgg aacaatttaa
    16141 atagcaacta actgataaat aaggatataa aagcaatgat aaaacatctt accttaccga
    16201 aaaaaatggc tgcaatggta cttcttgcag gcgctgccct cagcattgcg cctgtacagg
    16261 ctgcctccta cccaaccaaa cagatcgagt tagtcgttcc ctacgctgcc ggaggcggta
    16321 cggatctggt tgcccgtgcc tttgctgatg ccgccaaaaa ccatttaccc gtcagcatcg
    16381 gggttatcaa taaacctggc ggaggcggtg ctatcggcct gagtgaaatc gccgctgccc
    16441 gccctaacgg ttacaaaatt ggtttaggca cggttgaact gaccaccctt cccagcctcg
    16501 gaatggtgcg ttttaaaacc agcgacttta aacccattgc ccgtctgaat gcggatccgg
    16561 ctgctatcac agtccgtgcc gatgcgccgt ggaatagcta tgaagaattt atggcttact
    16621 ccaaagcgaa tcccggaaaa gtacgcattg gtaactcagg caccggagct atctggcatc
    16681 tggcggtagc tgcactggaa gacaaaacgg gcacaaagtt ttctcatgtc ccgtatgacg
    16741 gcgcagcccc tgccattaca ggcctgttag gcgggcatat tgaagcggtt tccgtaagcc
    16801 caggagaagt tatcaaccat gtgaatggcg gcaagctgaa gacactggta gtgatggcgg
    16861 atgagcgaat gaaaaccatg cctgacgtcc cgacgttaaa agagaaaggc gttgatctct
    16921 ccatcggcac ctggcgcggc ctgattgtgt cgcaaaaaac gccgcaggat gtggtggatg
    16981 ttctggcaaa ggcagcaaaa gagacggctg aagagcctgc attccaggat gcactgcaaa
    17041 agttgaatct caactatgca tggcttgacg ctgccagctt ccagacccaa atcagcgaac
    17101 aggaaaagta ctttgacgag ttgctgactc gcctgggcct gaaaaaataa acctgaggta
    17161 cggttgatga acacaaaaca gtctgttgct caactcgccg taccgcaccg caagcgcctt
    17221 tcatcaacga tggtggtggc gctgttactt tgtgtggttg ctggcgcggt gatgattaat
    17281 gccgctgatt ttccagcaac tgccattgaa acggatcccg gtgcaagtgc cttccctacc
    17341 ttctatgcct gtgccctgat tgtgctcgct gtcttgctgg tgatacgcga tcttttgcag
    17401 gcaaaaccag cctcttgcgc caacgcacag gaaaaaccgg cattcaggaa aacagcaaca
    17461 ggaattgcgg caaccgcgtt ttatattgtg gcgatgagct actgcggtta tctcattact
    17521 actcctgttt tcctcatcgt cattatgacg ttgatgggct acaggcgatg ggtactcaca
    17581 ccgggtattg cgctgctgtt aacggcaatc ctctggttgc tgtttgtcga agcgttacag
    17641 gtgccattgc ctgtcggcac atttttcgaa taacaggggc cagcaatgtt tgaaagtgaa
    17701 cttttaactc agggtttttc gacattactc aataatcctc aggcgctgct gtttgccact
    17761 tttggggtga tgctgggtat tgtgattggc gctttgcctg gtctgacagc gaccatgggt
    17821 gtggcgattt tgctgccttt cacctacggc atggagcctg tttctggctt gttgatgatt
    17881 tgcggcgtct tttttggtgg cgtctacggt ggttctatca ccgcaatttt gctcaaaatt
    17941 cccggaacgc ctgccgccgc agccaccgct attgatggtt atgagctgac gaaacaggga
    18001 aaagcagggc tggcattgag tgccgccacg ttctcttcct ttagtggcgg aacgctcagc
    18061 attatcgtgc tgatgtttct ctctccggta cttgccagtt gggcgctgaa atttagtgcc
    18121 tcggagtcct tcgccctggc aaccttcgga ctgagtatta ttgccagtat ttccggtgag
    18181 tcgctgatta aagggctgat tgccggggtt ggcggattgc tgatcgcaac gataggcctt
    18241 gatccaatgg gcggttttcc acggtttaca ggtggatttg tggagctgat gaatgtgcca
    18301 tttatcccgg tgatgattgg tttatttgct gcttcagaag cattccgctc tatggagcaa
    18361 aaccagcaaa ttcgtcaggg ggcgaaggtg gctatcggca gtctgttact gccctggcaa
    18421 acactacgcc gcattgcatt aaccattttg cgctcatcag gattaggggt ttttatcggc
    18481 atgatccccg gtgcgggggc ggatattgca gcttttgttg cctataacga aactcgtcgt
    18541 ttcagtaaga caccagaaaa ctttggtaag ggtgaaatta aagctgtggc ctcctgtgag
    18601 gcaggcgcga atggctgcac cggtggcgca ttgttaccta tgctgacgct ggggatccct
    18661 ggcgatgcgg taaccgccat catgctgggc gcgttaacgt tacagggaat gcaaccaggc
    18721 ccgctgatgt ttaccgacca cggcgatatg gtttatacac tgtttgtcgg catgatcttt
    18781 tgttacttca tgctgctagt tcttggactg ctctctttga aagtcatcgg taacgtggtg
    18841 aagatccccg gcaatattct cacaccgatg atcctcgcac tttgtgtggt cgggacttat
    18901 gcgttgaaca atagcctgtt tgatgttggc attatgctga ttgcaggcgt ggtgggctat
    18961 ttcatgcaga aaggaggata tccggcatca cccgtagtgc tggcattgat tatgggcccg
    19021 atggcagaaa gtaattttcg ccgtgcgctg tcgctttcag ggggttcact ggactttctg
    19081 tatacccgac cgatcactct ggcattgcta actctggcag cctttacgct actgacgcca
    19141 ataatccgca aaataatgcg tttacggcgt cagtaactga tatcgctccc cgaagttggg
    19201 gagcgttttt tatttcagca gacgacgcgc cgcttccacc acgattttca ccgcatggct
    19261 ctcggtttgt ttcatcgtct cagcattcgg gatctcttgc tgggtgcggt taacgataac
    19321 ccccgctacc ataccggcac gcaggccctg acttgcacac atggtcagca gggttgcaga
    19381 ttccatttca tagttcatta cgcccatcgc ctgccactct tccatagaac ctttaaagtg
    19441 acgaactacg cgaccagagt aagtatcgta acgttcctga cccgggtaga aggtatcaga
    19501 agaagccgtc acaccaacgt gagtggtggc accaattgac ttcgccgctt caaccagcgc
    19561 agtcgtacat tcgaaatcag cgacagccgg gaattccagc ggtgcgaagt gcaggctcgc
    19621 gccgtccaga cgaacagacg ccgtggtaac caggacatca cccacattaa tatgcggctg
    19681 aatagcgccc gttgtaccga tacgcaggaa ggtgcgaatg cccagctgtg ccagctcttc
    19741 aacagcaata gaggtagacg ggccgccgat accggtagag cagacgataa caggtttacc
    19801 atccagctct gcacgccagg tggtgaattc gcggtgagat gccagcttaa ccggcttatc
    19861 catcagcgcg gcgatctttt ccacacgatc cgggtcgcca gggacgatgg caagcgtagc
    19921 cccttgtaaa tcgtttttag tgaggccgag atgaaaaaca tcagacttgg acatatacaa
    19981 ctcctctgtg aatcggttta gtcagaagaa gcaaaaagac actttaccga agggtttaac
    20041 attttttcgt gatactcatc accatgacgc aaatgcgttg cataaattta cccatcaaaa
    20101 gtgatgcaaa tcacataaat cactcttcat tctgcaggac tgcataaaag atgagccgat
    20161 tcaggcaatg tgatttgttg catgctgact atagttaaca ttgcgcaaca tttttataag
    20221 ggtacggaga ataccatggc aacaacacaa caatctggat ttgcacctgc ggcatcgcct
    20281 ctcgcttcga ccatcgttca gactccggac gacgcgattg tggcgggctt cacctctatc
    20341 ccttcacaag gggataacat gcctgcttac catgccagac caaagcaaag cgatggccca
    20401 ctgccagtgg tcattgtagt gcaggaaatt tttggcgtgc atgaacatat ccgcgacatt
    20461 tgtcgccgtc tggcgctgga ggggtatctg gctattgcac ctgaacttta cttccgcgaa
    20521 ggcgatccga atgattttgc cgatatcccc acgttgctta gcggtctggt agcaaaagtg
    20581 cctgactcgc aggtgctggc cgatctcgat catgtcgcca gttgggcttc ccgcaacggc
    20641 ggcgatgttc atcgtttaat gatcaccgga ttctgctggg gtggacgtat cacctggctg
    20701 tatgccgcgc ataatccaca gctaaaagcc gcagtggcgt ggtacggcaa actgacgggc
    20761 gacaagtcgc tgaattcacc gaaacaacct gttgatatcg caaccgatct taacgcgccg
    20821 gttctcggct tatatggtgg tcaggataac agcattccgc aggagagcgt tgaaaccatg
    20881 cgccaggcgc tgcgggctgc taatgcgaaa gcagagatta tcgtctaccc ggatgccggg
    20941 catgcattca acgccgatta tcgcccgagc tatcatgccg aatctgcgaa agatggctgg
    21001 cagcgtatgc tggagtggtt taagcagtat ggtgggaagc ggaattagtt tcctcttagt
    21061 ctatatttga gatggtgagt gaacgagtgc attttgctgc catctctgtc ccccaaaaaa
    21121 taatcagtat gaattgattg gagagcacga ctccgctatt tgaccttaag aaagtcggca
    21181 agtcatagtc ataaatgagt ctcttcactg gcagacacct tagagattta ccatcaactc
    21241 ccattctcaa acaaacatta attattatca acacattcac at
//
LOCUS       DAZKJR010000089        20866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKJR010000089 DAZKJR010000000
VERSION     DAZKJR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.089"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..723
                     /gene="ais"
                     /locus_tag="ACHOWS_003309"
     CDS             121..723
                     /gene="ais"
                     /locus_tag="ACHOWS_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFQ0048841.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(762..1187)
                     /gene="nudI"
                     /locus_tag="ACHOWS_003310"
     CDS             complement(762..1187)
                     /gene="nudI"
                     /locus_tag="ACHOWS_003310"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFQ0048842.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            1466..2008
                     /gene="yfaZ"
                     /locus_tag="ACHOWS_003311"
     CDS             1466..2008
                     /gene="yfaZ"
                     /locus_tag="ACHOWS_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFQ0048843.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2108..3310
                     /gene="yfaY"
                     /locus_tag="ACHOWS_003312"
     CDS             2108..3310
                     /gene="yfaY"
                     /locus_tag="ACHOWS_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFQ0048844.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLTRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEKQAMEKLWLDVKRVAGQSVIFEGTEGLPSQISRELQTRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3530..4312
                     /gene="yfaX"
                     /locus_tag="ACHOWS_003313"
     CDS             3530..4312
                     /gene="yfaX"
                     /locus_tag="ACHOWS_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0048845.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     IWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4327..5532
                     /gene="rhmD"
                     /locus_tag="ACHOWS_003314"
     CDS             4327..5532
                     /gene="rhmD"
                     /locus_tag="ACHOWS_003314"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFQ0048846.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VISFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYNH"
     gene            5589..6878
                     /gene="yfaV"
                     /locus_tag="ACHOWS_003315"
     CDS             5589..6878
                     /gene="yfaV"
                     /locus_tag="ACHOWS_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0048847.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6895..7698
                     /gene="yfaU"
                     /locus_tag="ACHOWS_003316"
     CDS             6895..7698
                     /gene="yfaU"
                     /locus_tag="ACHOWS_003316"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFQ0048848.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAADKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGNY"
     gene            complement(7739..7972)
                     /locus_tag="ACHOWS_003317"
                     /pseudo
     CDS             complement(7739..7972)
                     /locus_tag="ACHOWS_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7973..8968)
                     /locus_tag="ACHOWS_003318"
     CDS             complement(7973..8968)
                     /locus_tag="ACHOWS_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFQ0048849.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDSEGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKIEGWQ
                     EGKLEGLQEGKLDGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(9161..10351)
                     /gene="glpC"
                     /locus_tag="ACHOWS_003319"
     CDS             complement(9161..10351)
                     /gene="glpC"
                     /locus_tag="ACHOWS_003319"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFQ0048850.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10348..11607)
                     /gene="glpB"
                     /locus_tag="ACHOWS_003320"
     CDS             complement(10348..11607)
                     /gene="glpB"
                     /locus_tag="ACHOWS_003320"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFQ0048851.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLEALRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQL"
     gene            complement(11597..13225)
                     /gene="glpA"
                     /locus_tag="ACHOWS_003321"
     CDS             complement(11597..13225)
                     /gene="glpA"
                     /locus_tag="ACHOWS_003321"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFQ0048852.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQRIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTMSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13498..14856
                     /gene="glpT"
                     /locus_tag="ACHOWS_003322"
     CDS             13498..14856
                     /gene="glpT"
                     /locus_tag="ACHOWS_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFQ0048853.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            14861..15937
                     /gene="glpQ"
                     /locus_tag="ACHOWS_003323"
     CDS             14861..15937
                     /gene="glpQ"
                     /locus_tag="ACHOWS_003323"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0048854.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            16140..16790
                     /gene="inaA"
                     /locus_tag="ACHOWS_003324"
     CDS             16140..16790
                     /gene="inaA"
                     /locus_tag="ACHOWS_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFQ0048855.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKSEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(16844..17098)
                     /gene="yfaE"
                     /locus_tag="ACHOWS_003325"
     CDS             complement(16844..17098)
                     /gene="yfaE"
                     /locus_tag="ACHOWS_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFQ0048856.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(17098..18228)
                     /gene="nrdB"
                     /locus_tag="ACHOWS_003326"
     CDS             complement(17098..18228)
                     /gene="nrdB"
                     /locus_tag="ACHOWS_003326"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0048857.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(18294..20579)
                     /gene="nrdA"
                     /locus_tag="ACHOWS_003327"
     CDS             complement(18294..20579)
                     /gene="nrdA"
                     /locus_tag="ACHOWS_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0048858.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            20707..20850
                     /gene="ypaB"
                     /locus_tag="ACHOWS_003328"
     CDS             20707..20850
                     /gene="ypaB"
                     /locus_tag="ACHOWS_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFQ0048859.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     VIS"
BASE COUNT         4836 a         5156 c         5624 g         5250 t
ORIGIN      
        1 tgaggcattt tgccggcatc gtaggatttt taatattaca ccaactgcta attatcgcca
       61 gaaatgtcgc tcaatttcac ggtaattgtc tggttgcgct tgtctatagg tggagtttac
      121 gtgttagctt tttgccgctc ttcgttgaag tcaaaaaaat atttcatcat tttactggcg
      181 ctcgctgcaa ttgccggact gggtactcat gccgcctgga gtagcaatgg tttgccacgt
      241 atcgacaata aaacgctggc cagactggca cagcagcatc cggttgtcgt tttgtttcgc
      301 catgctgaac gttgcgatcg ttcaaccaat caatgcttgt cagataaaac aggtattacg
      361 gtcaaaggta cccaggatgc ccgtgaactg ggcaacgctt ttagtgctga tatccctgat
      421 ttcgatctct attccagtaa taccgtccgg accattcagt cggcgacctg gttttcagcg
      481 ggtaaaaagc tgacggtaga taaacgactt cttcaatgcg gtaatgagat ttatagtgca
      541 attaaagact tacaacgcaa agcgcctgat aaaaatatcg ttatttttac ccataatcat
      601 tgcctgacat atattgctaa agataagcgt gatgcgacat ttaaacctga ttatctggat
      661 ggtttagtca tgcatgtgga aaaaggcaaa gtttatctgg atggggaatt cgttaaccac
      721 taaattttca atctgacagc cagtgatggc tgtcatcatt attacagaag acctttcaaa
      781 cgtaacgttt ttcgggtggc gacattcaaa tcatagtgcg ccagatcttc aggttttacc
      841 cacgcgtagt cttgaaactc ttcgtttatt ttcacttctc ggttggcaga aacgcagtca
      901 aaaatcaggt aaatcatata aatctcttcc ttgcgaccat ctgcatacgt cttggtgcga
      961 atatcatcgc tgaaggtcca cggcgtgatt tctgtcaaaa gcagctgttc tcccagctct
     1021 tcgcgaattt cgcggcgtag tgcctcttca attcgttcgc cgggctccac gccgccaccc
     1081 gaaagcgccc attgaccggg gaaaacgccg cgatcgtcgg ccattttaca cagcaaataa
     1141 gcaccatcat tttgaatcaa agggcataca atagtccgtt gtcgcacagg ggtattcctt
     1201 ataaataaaa agaaaccatg agaatacggt aactcagaaa tatgtgaaag tgacagtaat
     1261 ctcttttgcg atgataactg cacttattat tatttttgtg tgtatcggta aactgtgttt
     1321 attttttcgg cgtttaatta tttaagaagt atttagttac aataagtgac tagatccctt
     1381 ttttcgctca ttgaatcctc tgcgccaggc agtacaatcc cgcagctttg ggaattttga
     1441 ttaattgctt gtgggaaagg ggataatgaa aaaaattgcg ctagcaggtc tggccggaat
     1501 gctgttggtt tctgcatcgg tcaatgcaat gagcatcagc ggccaggcgg gtaaagaata
     1561 caccaatatt ggtgtcggtt ttgggactga atcgacgggc ctggcgttaa gcggtaactg
     1621 gacacataac gacgacgacg gtgacgtcgc gggcgtgggg ctggggttga atctgcctct
     1681 cgggccgtta atggcgaccg ttggcggaaa aggcgtttac accaacccga attacggcga
     1741 tgaaggttat gccgcagcgg taggaggtgg tttgcagtgg aaaattggca acagcttccg
     1801 cttgtttggc gagtattact actctccgga ttcgctctcc agcggtattc aaagttatga
     1861 ggaagcgaat gctggcgcgc gttacaccat tatgcgtcca gtcagtattg aggcgggtta
     1921 tcgctacctg aacctttctg gtaaagacgg taaccgcgac aacgcagtgg ctgacggccc
     1981 gtatgtgggg gttaacgcca gtttttgatc ccttccggcg cggcattctg ccgcgccatt
     2041 cagtttctta ctacgcctta tctccctttg cgttatagtg attcgataac aaaagcagga
     2101 gataaccatg ttaaaagtgg aaatgttatc caccggggat gaagtgttac acgggcaaat
     2161 cgttgacact aacgctgcct ggctggccga ttttttcttt catcaggggt tgcctttaac
     2221 tcgccgcaat acggtggggg ataaccttga tgacttagtg accattcttc gcgaacggag
     2281 tcagcacgcc gatgtgctga tcgttaacgg cgggctgggg ccgaccagtg atgatttaag
     2341 cgcactcgcc gctgcgacag caaaaggtga aggcctggtg ctgcatgagg cctggctcaa
     2401 agagatggaa cgctatttcc acgaacgtgg acgagtaatg gcaccgagca accgtaaaca
     2461 agcggagctg cctgccagtg ctgaatttat caataacccg gtaggcaccg cctgtggttt
     2521 tgccgtgcag cttaatcgtt gcctgatgtt ctttaccccc ggcgtaccgt cagaatttaa
     2581 agtgatggtc gagcacgaaa tcctgccgcg cctgcgcgag cgtttttctt taccgcagcc
     2641 gccggtttgt ctgcgtttga ctacatttgg tcgttcggaa agcgatctgg cacaaagcct
     2701 ggacaccctg caactgccgc cgggcgtaac aatgggctat cgctcttcta tgccgattat
     2761 cgaactgaaa ctcaccggac cggcaagcga gaaacaggcg atggaaaaac tgtggctgga
     2821 cgtcaaacgt gttgccggac agagcgtgat tttcgaaggt accgaaggac tgccctcgca
     2881 gatcagccgc gaattgcaaa cccgccagtt cagcctgacg ttgagcgagc aattcacagg
     2941 tggtttattg gctttgcaac tttctcgcgc aggtgctcca ttgctggcgt gtgaagtggt
     3001 tccttcacag gaggaaaccc tggcgcaaac tgcgcactgg attacagaac ggcgggccaa
     3061 ccattttgcc gggctggcac tggctgtttc gggtttcgag aacgagcatc tcaactttgc
     3121 gctagccacg ccagatggca ctttcgctct gcgtgtgcgt ttcagcacta cacgctacag
     3181 cctggctatc cgtcaggaag tgtgcgcaat gatggcactg aatatgctgc gccgttggtt
     3241 aaacggccag gacatcgcca gtgagcatgg ctggattgag gttgttgagt ccatgacctt
     3301 atctgtctga ttccgccact ctgtgccggt ttactgcacc ggcacattcc ccctccggca
     3361 aaatttgtcc aacctgttgt actcaataag aaaaaatccg tgcgctaatt cacaaatttg
     3421 tgcgtaattt ctccagtccg ctggacgagg agcgggattt gatgagcttc acaattttac
     3481 tccacctgag caacgagact gattccagta tctaagactg gagtccagta tgttggaatc
     3541 aagcaaagtt cccgccctga cacgggctat tgatattctc aatttgattg cccgcattgg
     3601 cccctgtagt gctgcgacga tcattgacac attggggatc cctaaaagca cggcctattt
     3661 gctgcttaat gaactcaggc gtcagcgttt tcttagtctt gatcaccagg aaaatttttg
     3721 tttgtggacc aggctggtgg agctttccgg tcatgcgtta agcaagatgg atctccggga
     3781 actggctcga ccgcgtttga cgcaactgat ggataccacc gggctgttat gtcacctggg
     3841 gattattgat aacggcagcg cttattacat tttgaaagtg gagtcatcag ccactatcag
     3901 cgtgcgttcc catgaaggaa aaagcctttc gctttatcgc tccggaatag gtaaatgcct
     3961 gcttgcctgg caacctgcag ctgtacagca aagtattatc gaagggctga tctgggagca
     4021 ggcgacaccc acaacgatta ctcatccaca gcaacttcac gaagaactgg cgcgtattcg
     4081 ccgtcagggc tggagctatg acaacggcga agattacgcc gatgtccgtt gtgtcgctgc
     4141 gccggtgttt aacgccaata acgagcttac cgctgcgatc tcggtggtcg gtacccgctt
     4201 acaaatcaat gaagaatacc gtgattatct ggcgggcaag gccattgcct gcgccaggga
     4261 tatttcacgt ctgttgggat ggaaaagtcc ctttgactta caggcctcat aacaatggag
     4321 aacagcatga ccctaccaaa aatcaaacag gttcgcgcct ggtttactgg cggtgcgaca
     4381 gcagaaaaag gcgctggcgg cggtgattat cacgaccagg gggcaaatca ctggattgat
     4441 gatcatattg ccaccccgat gagtaaatac cgcgattacg agcagtcacg ccagtcattt
     4501 ggcattaacg ttcttggcac gttgattgtt gaagtcgaag cagaaaacgg ccagaccgga
     4561 ttcgccgttt cgacagccgg tgaaatgggc tgttttattg tcgaaaaaca ccttaaccgt
     4621 ttcattgagg gtaaatgtgt cagtgatatc aaactgatcc acgatcaaat gctcaatgcc
     4681 accctgtatt actccggctc tggtggcctg gtgatgaaca cgatttcctg tgtcgatctg
     4741 gctctgtggg atctgttcgg caaagtggtc gggcttccgg tttataaact tttaggcggc
     4801 gcagttcgtg atgagattca gttctacgcc acaggtgcgc gtccggatct ggcaaaagag
     4861 atgggcttta tcggtggcaa aatgccgacg cactgggggc cacatgatgg cgatgcgggg
     4921 atccgcaaag atgccgctat ggtcgcggat atgcgtgaaa aatgcggtga ggatttctgg
     4981 ttaatgctcg actgctggat gagtcaggac gtgaactatg ccaccaaact ggcccacgct
     5041 tgcgcgccct ataacctgaa atggatcgaa gagtgcctgc cgccacagca gtatgaaggt
     5101 tatcgcgaac tgaaacgcaa cgcgccagtc ggaatgatgg tcaccagcgg tgagcatcac
     5161 ggcacactgc aatctttccg tacgctttca gaaaccggta tcgacattat gcagccggat
     5221 gttggctggt gcggtggctt aaccacgctg gtggaaattg ccgcaatcgc caaatccaga
     5281 gggcaactgg tggtgccgca cggttcgtct gtttactctc accatgcggt gatcagcttc
     5341 accaatacgc cattcagcga attcctgatg accagcccgg attgttcaac gatgcgtccg
     5401 caatttgatc cgattctgct taatgagcca gtgccggtga atggtcgtat tcataaatca
     5461 gtgcttgata aacccggttt cggcgtcgaa ctcaatcgtg actgcaattt gaaacgcccc
     5521 tacaaccact aatcactccg ggcgttgctg tgacaacggt aacgcccaca tattctaagg
     5581 agctttttat gagcaccgct ttgcttgacg ccgtggtgaa gaaaaaccgt gcgcgtttaa
     5641 ttccgtttat gttggcgctg tatgtgctgg cgtttctcga ccgttcgaat atcggttttg
     5701 ccaaacagac ctaccagatt gataccgggc tgagtaatga agcttatgcg ctgggagcag
     5761 gcattttctt tgtggtatat gcgtttctag gtgttccggc gaatcttttg atgcgcaaac
     5821 tgggggccag aacctggatt ggtacgacaa cactgctgtg gggatttctt tcggcagcca
     5881 tggcatgggc cgatactgaa gcgaaatttc tgatcgttcg cactctgctt ggtgctgcgg
     5941 aggccgggtt cttccctggt atgatttatc tcacctcgca atggtttccg cagcgtaatc
     6001 gcgccagcat tatggggctg ttctatatgg gcgcaccgtt ggcgttaaca ctgggatcac
     6061 cgctttccgg cgcgctgctg gagatgcatg gatttatggg acatcccggc tggttctgga
     6121 tgtttgtgat tgaaggattg ctggcagtcg gcgctggggt attcacattc ttttggcttg
     6181 atgacacacc ggagcaggca cgttttctga gtaaagaaga aaaaacattg cttatcaatc
     6241 aactggcaag tgaagaacaa cagaaagtga cttctcgact gagcgatgcg ctgcgtaatg
     6301 ggcgagtctg gcaactggcg attatctacc tgaccattca ggtggcggtt tacggattaa
     6361 ttttcttcct gccgacccag gttgctgcat tgctggggac aaaagtgggc tttacggcgt
     6421 cggtggtcac cgccattccg tgggttgcgg ccttgtttgg gacctggctt attccgcgct
     6481 attccgataa aaccggcgaa cggcgtaatg tcgcagcgct gacattactg gcggcaggca
     6541 ttggtattgg tctctccggg ctgctttctc cagtactggc gatcgtagcg ctgtgtgttg
     6601 cagccatcgg gtttattgcc gtgcagccgg tgttctggac gatgccgaca caactgcttt
     6661 ccggtacggc gctggcggcg gggattggtt ttgtaaacct gtttggtgca gtgggtgggt
     6721 ttattgcccc gatcctgcgc gtgaaagcag aaacgttgtt tgccagcgat gcggcgggat
     6781 tactgacgct ggcagcggtg gcggtcatcg gttcgctgat tattttcact ctgcgtgtaa
     6841 atcgcactgt tgcgcagacc gacgtggcac atcattaata ggttaaggaa cacgatgaac
     6901 gcattattaa ccaatccctt taaagaacgt ttacgcaagg gcgaagtgca aattgggctg
     6961 tggttaagct caacgactgc ctatatggca gaaattgccg ctacttccgg ttatgactgg
     7021 ttgctgattg acggggagca cgcgccaaac accattcagg atctttatca ccagctacag
     7081 gcggtagcgc cctatgccag ccaacccgtg atccgtccgg tggaaggcag taagtcgctg
     7141 attaaacaag tcctggatat tggcgcgcaa actctactga tcccgatggt cgatactgcc
     7201 gatcaggcac gtcaggtggt gtctgccacg cgctatcctc cctacggtga gcgtggtgtc
     7261 ggggccagtg tggcacgggc tgctcgctgg ggacgcattg agaattacat ggcgcacgtc
     7321 aacgattcgc tttgtctgtt ggtgcaggtg gaaagtaaaa cggcactgga taacctggac
     7381 gaaatcctcg acgtcgaagg gattgatggc gtgtttattg gacctgcgga tctttctgcg
     7441 tcgttgggct acccggataa cgccgggcac ccggaagtgc agcgaattat tgaaaccagc
     7501 attcggcgga tccgtgctgc ggataaagcg gctggttttc tggctgtggc tcctgatatg
     7561 gcgcagcaat gcctggcgtg gggagcgaac tttgtcgctg ttggcgttga cacgatgctc
     7621 tacagcgatg ccctggatca acgactggcg atgtttaaat caggcaaaaa tgggccacgc
     7681 ataaaaggta attattgata tcaaaggccc atgggggtcg gctgtgggcc tgtgttaatt
     7741 agtggttatt cgctgccaga tcggcttcgc ttagctgggt ggctgcgagc accaggtcac
     7801 gatcaatgcc ctgtaccagc atgcgcaaag caattttaat ggcttgttta cgcatacctt
     7861 cctgcaaacc ttctaattta ccctcctgcc agccttcaag cttaccttcc tgccagccaa
     7921 tctgatgccc ttcctgcctc agccgttcag caatagtcat caatctctcc ttttaatgac
     7981 agttcggaat atcagcatca gtgagttggg ttgtcgccag cacaatctca cgttcaaagc
     8041 cctgctccag catgcgcaag gcaattttaa tggcttgttc atgcatgcct tcctgcaaac
     8101 catctaattt accttcttgc aaaccttcta atttaccttc ctgccaacct tcaattttac
     8161 cttcctgcca gccttcaatc ttaccttcct gccagccaat ttgatgccct tcctgccgca
     8221 gccgttcagc aatagtcatt aatctctcct tttgtagtgg tgaacgttcg gcaatctcct
     8281 caataaaacg ggtgaaacgg gaggtatcgc cgcattgcag cagataatta aacagcgttt
     8341 gtagctggct gtcattagtg aagcctctaa ccaaaagcgt ggtgatcctg tcgaccatac
     8401 cgattaaatc gcggtcgcga atatgctttt gaatcagttc aagcagagct atacgccgat
     8461 gttgcatgat ctcatcgtca ggtacgatgg tgatatccac caacggaaaa gcttcggtgt
     8521 acaactgccg ggcaagttgc ggatcgtcaa actcatccag ccagttgagt gagtacgggt
     8581 agggcgaggt ttcgccatga taaaacagca atggcaccac cagcggaact ctgtcatagc
     8641 ctttatccag gtgacgctgc atggcggcag tggcatagcg cattagccga aaagccatat
     8701 tcttttctgc agagctttga tgttcaatca cgcagtagat atagccttca ctgtcgctgg
     8761 tttccacaga ccacaaaaca tccgagtagt aagcgcgtaa acttttttca ataaagctgg
     8821 tgggttccag gcgtaaagtt tgcaggttgc aaagcttgcg cagcggctct ggtagatgta
     8881 tttccagaaa atcccgagcg gtttcgggtg tgaacataaa ggttttaaac accgcatcat
     8941 gcggggagga ggttgttgat tcggtcatgg ttgtccgtca cctgatattt atgatgacgc
     9001 gacattacgg ttatcccagg cctttgacat ctgccagttt cagcctttgc gagacgtttt
     9061 cagaggaatt ttcactgcgt tgcaacgcat acattgggca cggaatcgtg tacgcaaaaa
     9121 tgcccggtaa caacatgctt accgggcatc agaaaggagt ttaagccagc gcctgggcca
     9181 gcagcgtaat cggatgttcg cagcgaagac ttgtggacat ctcaatctgc catttgcagg
     9241 tttcgcagtc ggtaaccacc agatctgcgc cgctttcttc tatctgacgg aacagtggtg
     9301 cgccgatggc ttgtgaggtg gggtagttct cttttttgaa accgtaagta cctgcaatac
     9361 cgcagcactg ggaatccagc accgttaact caagccccgg gattttacgc aacagctcca
     9421 gggtgtagag cgtccagccc attttttcca tatggcacgg agtgtgataa accactttca
     9481 gcggcagcgg tttcagcggc aaggttttgc cttcgtccag cttgcgccac agccagcggg
     9541 tcgccagttc gatgtgatcg cgcaagcctt tgttgtcgac attcagcact tccggatatt
     9601 cgtcgcgcag ggcaaatgta caggttgagg aggtggcaat caccggaatg ccttttactc
     9661 ccactgcttc gcggatcgac tctacatttg tgattgcctg tttacgtgct ttatcggtaa
     9721 agccgttggc gatcagcggt acgccgcagc atttttcttt gctgagcagt tgtacaccgg
     9781 tacccattgc gttgagcact ttaattaaat ctttgcctaa ctgcggatgg ttgtagttaa
     9841 cgaagcagcc gtgaaagaaa gccacctggt ctttatattg tgcctgctgg gccgccacgc
     9901 tgcgatacca gcgacggaac gtaccgaagg agtatttcgg tagcgtgcgg cgatgatcga
     9961 tttttaacgc cgcatcaagc agctgccgca ctggtttcag cgaggtagcg gtattgacga
    10021 ttggtgcgaa tggcgtggaa acgctaccca tcaggtcggt atgactcaac acaaaattgc
    10081 gcagcgacgg gcgtgtggtg tcatatttcg cccgcgcgcg ctggatgata tcgccaatct
    10141 tcacatcgga cgggcaggcg acttcgcagc gtttgcagtt gatgcaatat ttcagcgctt
    10201 cgtcatacag tgcgccatct ttcagacgca gacgctcgcc atccggcccg gcttgttttg
    10261 gccctggata gccgggattc acccggctca ccgggcaggc ggtggtgcag acggtgcact
    10321 taatgcagtt ttcgaagctg gtgtcgttca tagttggcct cctgcgcgtt gggcaatctg
    10381 ttgagcggca tgtaaagcac tgacggcaca aacaccgccg ccgcatccct gggcgatggg
    10441 atcaaatccg cccagcaccg aaccgatggc aaacaggttc tcaatggttt gccctgcctg
    10501 cgatgggcgt agtgcctcat cagtggttac accgaacagc tgccacggtt gcggcgcaaa
    10561 aaaatcgccc ttataccatt caccccgcgt ggcggtttgt agcacatcaa ggccgagaat
    10621 cggctcgcga atgccgtcac gttctgccac cagtccgccg ctaaagaaac tgccgctcgc
    10681 aagcaccgca aatcgtgggc gcagcgggat atcggcgtga ttgcgcgtcc agatttcatt
    10741 cactacgcca tttttacagg tcactttttt cacttcatcg cctggcatcc acacgccacc
    10801 ctggcgcaca aactggcgct gtaactggtt ttgcagacga atgcccagca cggaaggcgg
    10861 cagcgttggc aaaagcatca gtgaacaagg tagtttttca ttcaaccaac gccacagttt
    10921 gtcatcggcc aggccgaagc aggcgggcat caggatcatt tcgcaggtat tggcgacagg
    10981 aataagcgca tcaagtaaca gcgaccagtt ctcttcgtta tcaaggaaac gggcgatatt
    11041 caccgcgcga aactcggtgg cgttgttgcg cagcacatcc agttccggca gctctatttc
    11101 tgcggtttca acggcaaggt cgagttcgcg caacgaagcc gccgcaagat gcgcctgaaa
    11161 atccatcagg ccgcttatcc cgaccacaca tattttcttc gcgggcaacg gccagacagg
    11221 aacttctggc gaacttagcc aggttgagcg cagagtgccg agcggggtaa ctcgctggtg
    11281 ggccagttct acgctgccct gtaattgcgc accgctttca gcgattaatg cctgcgcctg
    11341 gcaagcgaga tcgagtacgc gttgtggccc gagaagagtg taaggatggg caggtgcctg
    11401 ctgacgcaaa gcttccagtc cactgtgaat gtctgtcacc ggttgaccat ccggcagatg
    11461 gctcagcaaa tccagcgatc cagatgagaa atggagcgcg ctttgaccac gcgtgacaat
    11521 ggcacagcgc aggccgtgtt tttgcagttg caggccacag agtaatccgg cgaggccgcc
    11581 gcccataatg acagtatcaa agcgcatctt tctgctcctt ctccagacca cacagtccct
    11641 gataaaccca gcgggtaaat tcgctttcgc gcagtgcatc tccccaggcg atgggttgca
    11701 cgcctttcca gcgttcgtta aggaaggtgg aaagttgctc gatagattgc gcggacatcg
    11761 tgacgttaaa acgttgcagc agtccggcag cgcggcaggc gcagagttcg ccctggcagg
    11821 tacccatccc cacacgggta cgacggcgta aatccagcag gctattaacg tttaaatttt
    11881 ctaccgcgta ctgaacttca cccgcagtta ccgcttcgca ctcacatacc aggctacggt
    11941 gcagacggcc ttcgctcagc caggcgggtg tgcgatcgcc atgacgataa accgcagaac
    12001 cgcgcagcgg ggcaggcagg gagatgactt tacgcaaggt aacttcagcg ggctcctgtg
    12061 aaccaggaag tgccagatcg gcggttgtac aggggcgcgt gttgcccagc ttgcgacata
    12121 ccgcgtcggt agcccattcc gccatcagcc gataggtcat cagtttgccg ccggtgatgg
    12181 tgataaatcc gtccagacca tcgcgttcag catggtcgag cagcacgatg ccacgactga
    12241 cgttacggcc gctcgggtcg tcatcgctgg caaccagcgg acgcacgcca gagtaagccc
    12301 gcaaaatacg cgttttcgcc atcacggggg ccagtttttc cccttcacgc agcagaatat
    12361 caacttcttc tgccgtcact cgattatcgt caatctcatt gtaatcaata cgtaaagagg
    12421 tggtgccaat tagcgaaatg gtatcgccag gcaccagaat atcggcgtcg gaaggtttgc
    12481 ggcagcggtt gatcacatgc tgattaatgc ggtgatccat gatcagcagc gatcctttcg
    12541 ccgggaacat gcgaatgcgc agatcggcat attcggcaat gcgttgcccc cagatcccag
    12601 cggcattaac cacgacaggc gcatgaaggg cctgagtttc gccggtgaga tggttacgta
    12661 cacgaacgcc gcacaccgtc gcaccttcac gaatcagccc cgtgacttca tgagcggtaa
    12721 ggataacggc accgtgttct ttggcatcca gcatgtttgc tgcggtcaga cgaaatggat
    12781 caacggtgcc atccggaact ttcaccgcgc caatcagtgc cgggttaacg gcaggttcga
    12841 taatgcgcgc ttgctgcggg tctatagctt ctgcgctgat ccctgcttct tcgcaggcgc
    12901 ggataaaagt ggcctggaag gagaggtcat cttccggcag ggtgataaac aggccgttgg
    12961 tcggttcaac gcagtgacgt gcaatgcgtt tcaggatctg gttttcacta atacattcgc
    13021 gggccgattc cgcatcggtt accgcatagc gcgcaccgct gtgcagcagg ccgtggttac
    13081 gcccggtggc accggttgcg atgtcgtggc gctcaaccaa aatcacgcgc agtccgcgca
    13141 gggcacagtc gcgggcaatc ccggctcccg ttgcgccgcc gccaatgata atcacgtcac
    13201 ttgattgcga gtcgcgagtt ttcattgtta tccctctgaa gttcgttttt taccatttag
    13261 ccatagtaaa aacatgaatt gtttgatttc gcgcatattc gctcataatt cgaaagtgaa
    13321 acgtgatttc atgcgtcatt atgaacattt aacccatcga atttaataat gtgtgcggta
    13381 attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa acggcaggtt
    13441 ctcccactga atcaggctgt taatcataaa taagaccacg ggccacggag gctatcaatg
    13501 ttgagtattt ttaaaccagc gccacacaaa gcgcgcttac ctgccgcgga gatcgatccg
    13561 acttatcgtc gattgcgctg gcaaattttc ctggggatat tctttggtta tgctgcttac
    13621 tatttggttc gtaagaactt tgccctcgct atgccttatc tggtcgagca gggattctca
    13681 cgcggtgatt taggttttgc cctttcgggg atctcgattg cttatggatt ttcgaaattc
    13741 atcatgggct cggtgtcgga tcgctcgaat ccgcgcgttt tcctgcccgc aggtttgatt
    13801 ctggcggcgg cggtgatgct ttttatgggc tttgtgccgt gggcgacgtc gagcattgcg
    13861 gtgatgtttg tgctgctgtt cctctgcggt tggttccagg ggatgggatg gccgccgtgc
    13921 ggtcgtacta tggtgcactg gtggtcgcag aaagaacgtg gcggcattgt gtcagtgtgg
    13981 aactgtgcgc ataacgtcgg tggtggtatt ccgccgctgc tgttcctgct ggggatggcc
    14041 tggttcaatg actggcacgc ggcgctctat atgcccgctt tctgcgccat tctggtggca
    14101 ctgttcgcct ttgcgatgat gcgcgatacc ccgcaatcct gtggcctgcc gccgatcgaa
    14161 gagtacaaaa atgattatcc ggacgactat aacgaaaaag cggaacagga gctgacggcg
    14221 aagcaaatct tcatgcagta cgtgctgccg aacaaactgc tgtggtacat cgctatcgcc
    14281 aacgtgttcg tttatctgct gcgttacggc atcctcgact ggtcaccgac ttatctgaaa
    14341 gaggtgaaac acttcgcgct ggataaatcc tcctgggcct acttcttcta tgagtatgcg
    14401 ggcattccgg gcaccctgct gtgcggctgg atgtcggata aagtcttccg tggcaaccgt
    14461 ggggccaccg gcgtcttctt tatgacactg gtgaccatcg cgactatcgt ttactggatg
    14521 aacccggcag gtaacccaac cgtagatatg atttgtatga ttgttatcgg cttcctgatc
    14581 tacggccctg tgatgctgat cggtctgcat gcgctggaac tggcaccgaa aaaagcggca
    14641 ggtacggcag cgggctttac cgggctgttt ggttacctgg gcggttcggt ggcggcgagc
    14701 gcgattgttg gctacacagt ggacttcttc ggctgggatg gcggctttat ggtaatgatt
    14761 ggcggcagca ttctggcggt tatcttgttg attgttgtga tgattggcga aaaacgtcgc
    14821 catcaggaat tactgcaaaa acgcaacgga ggctaatggc atgaaattga cgctgaaaaa
    14881 ccttagcatg gcgatcatga tgagtggaat gatcatggga agcagtgcaa tggcggcgga
    14941 cagcaacgaa aaaatagtca tcgcccatcg cggtgccagt ggatatttgc cggagcatac
    15001 gctgccagca aaagcgatgg cgtatgcgca gggagcggat tatctggaac aagatttggt
    15061 gatgaccaaa gacgaccatc tggttgtcct gcatgaccat tatctcgatc gtgttactga
    15121 tgttgccgat cgtttcccgg atcgggcgcg caaagacggt cgttactacg cgatagattt
    15181 cacgctcgat gaaattaagt cactgaaatt taccgaaggt ttcgatattg aaaatggtaa
    15241 gaaagtacag acttatccgg ggcgtttccc gatgggtaaa tctgacttcc gggtacacac
    15301 tttcgaggaa gagattgaat ttgttcaggg gttaaatcac tctaccggga aaaatatcgg
    15361 tatctatcca gaaattaaag cgccgtggtt ccatcatcag gaagggaagg atattgcggc
    15421 aaaaacgctg gaagtgctga agaaatatgg ttacagcggt aaggacgaca aagtttattt
    15481 gcaatgtttt gatgccgatg aactgaagcg tattaagaat gagctggaac ctaaaatggg
    15541 catggacctc aatctggtac agctgattgc ctataccgac tggaacgaaa ctcagcagaa
    15601 acagccggac ggaagctggg ttaattacaa ctacgactgg atgtttaagc cgggggccat
    15661 gaaacaggtg gcggaatatg ctgatggtat tggtccggat tatcatatgt tgattgagga
    15721 gacatcgcag ccaggtaata tcaaactcac tggcatggtg caagatgctc agcagaacaa
    15781 gctggtagtg catccttata ccgtgcggtc agataaactg cctgaataca ctactgatgt
    15841 gaatcagtta tatgatgctc tgtataacaa agcgggtgta aatgggctat ttactgattt
    15901 ccctgataag gcagtaaaat tccttaataa agagtaaatt aactaccgga tggcacttat
    15961 gtgctatccg ataccactct tcattaaagt ctgtgggtta tccttaattg caattatttt
    16021 gagcgagatg acttgttccc ggcataagat actcgtcaat acaacacgta ttattaagat
    16081 tttcctcagc taaacatcta tagtcattgt cgttaataaa aaacgacagg cgatagggta
    16141 tggcagtttc agcaaagcac gacgaattta atcactggtg ggcaacagag ggcgactggg
    16201 ttgaagagcc taactatcgc cgtaatggta tgagcggggt gcaatgcgtc gagcgcaacg
    16261 gcaaaaagct atatgtaaag cgcatgacgc atcatctgtt tcattccgta cgttatccgt
    16321 tcggccgacc aacgattgtc cgtgaggttg cggtaattaa agaactggaa cgggccggtg
    16381 tcatcgtgcc gaagatcgtt tttggtgaag cggtgaaaat tgagggggaa tggcgcgcgt
    16441 tgctggtgac tgaagatatg gcggggttca tcagcattgc tgactggtat gcccagcatg
    16501 cagtaacgcc ctattctgac gaagtacggc aagccatgtt gaaagcagtg gcgctggcgt
    16561 ttaagaaaat gcatagcgtt aatcgtcagc atggctgttg ctatgttcgc catatctatg
    16621 tgaaaacaga aggtaagtca gaagctggtt ttcttgatct ggagaaaagt cgtcgccgct
    16681 tgcgtcgcga taaagcgatt aatcatgact tccgccagct ggaaaaatat ctggagccga
    16741 tccctaaagc ggactgggag caggtgaaag cgtattacta cgccatgtaa tcgttgtagg
    16801 ccagataata cgcgtaagcg tcgtatctgg cgctagtacc cgatcacaac tcgatttcaa
    16861 tatcgccttt tgcccggcaa caacagggca aaatttcccc cggctgaata aaggctaacg
    16921 gttcggcaat ccagtccacc tggcctgcaa caaggcgcgt gcgacaggaa ccgcagtaac
    16981 cttcgcggca ctggtactca accgccacat tgtgggattc cagcgccgcc agaagggaag
    17041 ggtgttcatc ctggcacagc agttgtgtgc cagtgatgcg cagggtaacg cgagccatca
    17101 gagctggaag ttactcaaat cgtcggtgtc cacttccgag tcaatctgcc cgaccagata
    17161 agaactgact tccacttcct gcggagcaac ctgcacgtta tcagacacca gccaggtatt
    17221 gatccacggg atcgggttgg agcgtgtctg gaacggcaga tccaaaccga ctgcctgcat
    17281 gcggatattg gtgatgtatt caacgtactg gcagagaatg tctttattca gaccaatcat
    17341 cgaaccgtca cggaacagat aatccgccca gtctttctct tgctgtgccg cctggacaaa
    17401 caggtcatag cactcctgct tacattcttc ggcaatttcc gccatctccg gatcatccgc
    17461 gccgctgcgc agcagattca gcatatgctg agtaccggtc aggtgcaggg cttcatcgcg
    17521 agcaatcagg cgaataattt tggcgttacc ttccatcagt tcgcgttctg caaaggcaaa
    17581 ggaacaagca aagctgacgt agaagcggat cgcttccagc gcgttaacgc tcatcaggca
    17641 gagatacagt tttttcttca gctcgcgcag gctaacggtt acggttttac cgttaacggt
    17701 gtgggtacct tcgcccagca gatgccagta gctggtcatt tcgatcagct catcgtaata
    17761 gctggagatc ccttccgcac gtttctggat ctgctcgttg gtgacgatat cgtcaaacac
    17821 aacagacgga tcgttaacga tattacgaat gatatgggta taagaacgag agtgaatggt
    17881 ttctgagaac gcccaggttt cgacccaggt ttccagctca gggatcgaga tcagcggcag
    17941 cagtgccacg ttcgggctgc gaccctgaat ggagtccagc agcgtctgat atttcaggtt
    18001 gctgataaag atgtgttttt cgtgttcagg tagtgcctgg taatcgatac ggtcacgaga
    18061 aacgtcaact tcttccggac gccagaagaa agagagctgc ttttcgatca gcttttcgaa
    18121 gatgtcatat ttttgctgat cgtagcgagc cacgttaacc ggctgaccaa agaacatcgg
    18181 ttctttcagc tgatcatttt tcgtctgtga aaaggtggta tatgccatga atgtgtcctg
    18241 ttgggagttt aatgccggat aaggcatttt acgccgcatc cggcatctca atatcagatc
    18301 ttacatgcgc cgctttcgca gccatcgtcc tggattgacg gtaccagatc gtcttgtgcg
    18361 tcttcagcac cgtcacgggt gttctgataa tacagcgttt tgaccccgaa tttgtaggcg
    18421 gtgagcaggt ctttcagcag ttgctgcatc ggcacttttc ctgacgggaa gcgtgacgga
    18481 tcgtagttgg tgttagcaga gatcgactga tcgataaatt tctgcatgat acccaccagt
    18541 tgcaggtagc catcgttacc cggcatttcc cacagcagtt cataggcgtc gtgcagatgc
    18601 tcgtagtccg gcaccacctg gcgcagaata ccgtctttcg acgctttgat gctgacgtaa
    18661 ccacgcggcg gttcaatacc gttagtggcg ttagagatct gcgaagaggt ctcggacggc
    18721 atcagggcag aaagcgtgga gttacgcagg ccgtgcgttt tgattgactc acgcagtgct
    18781 tcccagtcgt aatgcagcgg ctcattagcg atggcatcca gatctttctt ataggtatcg
    18841 atcggcagga tccctttcgc gtaagtggtt tcgttaaacc acgggcacgc gccttgctct
    18901 ttcgccagct cattagacgc tttcagcagg taatactgaa tggcttcgaa ggttttatgc
    18961 gtcaggttgt tggcgctgcc gtcggagtag cgtttaccgt gcttcgccag gtagtaagcg
    19021 aagttgatca caccaatacc cagcgtacga cgacccatcg ctccacgttt ggcggccggg
    19081 atcgggtaat cctgataatc cagcagcgcg tcgagtgcgc gtactgccag aattgccagc
    19141 tcttccagtt catccaggct gttaattgcg cccaggttga aagcagacag cgtacacagc
    19201 gcgatttcac cgttctcgtc gttgacgtcg ttcagtggtt tggtcggtag ggcgatttcc
    19261 aggcacaggt tagactggcg cactggtgca atagccggat caaacgggct gtgggtgttg
    19321 cagtggtcta cgttctgaat atagatacga ccggtagacg cacgttcctg catcatcagc
    19381 gagaacagct caacggcttt cacacgctgc ttgcggatgc tgtcgtcttt ctcatatttg
    19441 gtgtacaggc gttcaaactc ttcctgatcg gcgaagaacg cgtcgtacag ccccggtacg
    19501 tcggacgggc tgaacagggt gatatcttca cctttcagca gacgggtata catcagtttg
    19561 ttgatttgta ccccgtagtc catatgacgc acgcggttgc cttcaacccc acggttgttt
    19621 ttcaacacca gcaggctttc cacttccaga tgccacatcg ggtagaacag tgttgccgca
    19681 ccgccgcgca caccgccctg agagcaggat ttcaccgctg tctggaaatg tttgtaaaac
    19741 ggaatacagc cggtgtggaa tgcttcgcca ccgcgaatcg ggctacccaa cgcacgaata
    19801 cgcccggcgt tgatgccgat cccggcacgc tgggaaacgt atttaacaat cgcgctggag
    19861 gtggcgttga tggaatccag gctgtcgccg cactcgatca gtacgcagga gctgaactga
    19921 cgagtcgggg tacgcacgcc ggacatgatt ggcgtcggca gcgaaatttt aaatgtggaa
    19981 accgcgtcgt aaaaacgctt cacgtattgc aggcgcgttt cacgcgggta gttcgagaac
    20041 aggcacgcgg caaccagaat ataaaggaac tgggcgcttt cgtagatttc gccggtcacg
    20101 cggttctgta ccagatattt gccttccagc tgcttaacgg cagcataaga gaaggtcata
    20161 tcacggtcgt gatcgataaa cgtgtccatc tgcttgaact cttcttccgt gtagtcttcc
    20221 agcagatgat tatcgtattt gcccatctcg accattttca ccacgtggtc gtacagcgca
    20281 ggcggctcaa actggccgta ggctttttta cgcaggtgga agatcgccag gcgcgcggcg
    20341 agatactgat aatccggcgc atcacgggag atcaggtctg cggcagcctt gataatggtt
    20401 tcgtggatgt cagaggtctt gataccgtca taaaactgaa tgtgggagcg cagttcgacc
    20461 tgggaaatcg aaacgttatg cagtccttct gccgcccaat ccagaacgcg atggattttg
    20521 tcgagattga tgcgctctgt gctaccgtcg cgctttgtca ccagcagatt ctgattcatg
    20581 tatgtcgtac ctgtttttgg aattctttcc gcaatagttt catgcagact acctgtagtg
    20641 agggaagctg cttagaacac tatatatagg gggtatgttt gggattcact gcaagatagt
    20701 gtgaaaatgg ccctcttgca agtgcataac tttgtggata actcaggaag gaaaaagtgg
    20761 ctttcgcgca ccttaggtca gacaaggtgt ccgggaaagt caatgggaag agaaaaattt
    20821 gttaaaaata actgttcggt gatttcttga acggcagaat aggttg
//
LOCUS       DAZKJR010000090        20386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKJR010000090 DAZKJR010000000
VERSION     DAZKJR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.090"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(452..1648)
                     /gene="hemY"
                     /locus_tag="ACHOWS_003329"
     CDS             complement(452..1648)
                     /gene="hemY"
                     /locus_tag="ACHOWS_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFQ0048860.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1651..2850)
                     /gene="hemX"
                     /locus_tag="ACHOWS_003330"
     CDS             complement(1651..2850)
                     /gene="hemX"
                     /locus_tag="ACHOWS_003330"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFQ0048861.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADAP
                     AAAPQGE"
     gene            complement(2872..3612)
                     /gene="hemD"
                     /locus_tag="ACHOWS_003331"
     CDS             complement(2872..3612)
                     /gene="hemD"
                     /locus_tag="ACHOWS_003331"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFQ0048862.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CVIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3609..4550)
                     /gene="hemC"
                     /locus_tag="ACHOWS_003332"
     CDS             complement(3609..4550)
                     /gene="hemC"
                     /locus_tag="ACHOWS_003332"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFQ0048863.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYESLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4937..7483
                     /gene="cyaA"
                     /locus_tag="ACHOWS_003333"
     CDS             4937..7483
                     /gene="cyaA"
                     /locus_tag="ACHOWS_003333"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFQ0048864.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     CNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7523..7843)
                     /gene="cyaY"
                     /locus_tag="ACHOWS_003334"
     CDS             complement(7523..7843)
                     /gene="cyaY"
                     /locus_tag="ACHOWS_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFQ0048865.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7891..9948)
                     /locus_tag="ACHOWS_003335"
     CDS             complement(7891..9948)
                     /locus_tag="ACHOWS_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048866.1"
                     /translation="MINTSRQDGFMHFRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGTR
                     TDDNGGENSLDIKTLHQVKFRSPWKPDFLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTTFNPCVAVPFGPWDTLARTRTKIIINGEQLKADPETFTVVRWMPGSLL
                     TWRDKSGLHRKVLNQGNLDKDRANSCAAFTLQEHSVSWRKGPECQQEVLPGLDPEQFH
                     PLSNTVAQYQDKLYTIKKTGFGENRLNIVTLDNPGLVIDKRFNAGRTHGYLLTKLPAD
                     GEEEDGLQVFDSSGPLILMDYRVPDEHEAHLSDSPHYKKWYAHDDRYVYAFDGSQLWR
                     YPTPNTKAVRVKWKTKDSGYGYWANYRSGELDGRLLEDGAFIPTGIPYNSSVNIAQYS
                     DGEGSFLVGETDVSWRKLRSTDQQWSQWEKLPDIDPKAFHPITDRVAQYKNHLYIAKL
                     SPFGEDTLEIITLDSSAPFLKQRINAGKGHGYFMRSSRLRDDIQIFAIDGPLKTTERF
                     AYDNRYVYTWIGSQLYRTASPCPARTRNLDQNMFSSNDDIIILTTEEECHKTPDIEQT
                     LKL"
     gene            complement(10290..10844)
                     /locus_tag="ACHOWS_003336"
     CDS             complement(10290..10844)
                     /locus_tag="ACHOWS_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFQ0048867.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11186..11389
                     /gene="yifL"
                     /locus_tag="ACHOWS_003337"
     CDS             11186..11389
                     /gene="yifL"
                     /locus_tag="ACHOWS_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0048868.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVEM
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11426..12250
                     /gene="dapF"
                     /locus_tag="ACHOWS_003338"
     CDS             11426..12250
                     /gene="dapF"
                     /locus_tag="ACHOWS_003338"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0048869.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12247..12954
                     /gene="yigA"
                     /locus_tag="ACHOWS_003339"
     CDS             12247..12954
                     /gene="yigA"
                     /locus_tag="ACHOWS_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFQ0048870.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDCWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12951..13847
                     /gene="xerC"
                     /locus_tag="ACHOWS_003340"
     CDS             12951..13847
                     /gene="xerC"
                     /locus_tag="ACHOWS_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0048871.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13847..14563
                     /gene="yigB"
                     /locus_tag="ACHOWS_003341"
     CDS             13847..14563
                     /gene="yigB"
                     /locus_tag="ACHOWS_003341"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFQ0048872.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14647..16809
                     /gene="uvrD"
                     /locus_tag="ACHOWS_003342"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14647..16809
                     /gene="uvrD"
                     /locus_tag="ACHOWS_003342"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFQ0048873.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16854..17756)
                     /locus_tag="ACHOWS_003343"
     CDS             complement(16854..17756)
                     /locus_tag="ACHOWS_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFQ0048874.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LKQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17839..18603)
                     /gene="yigE"
                     /locus_tag="ACHOWS_003344"
     CDS             complement(17839..18603)
                     /gene="yigE"
                     /locus_tag="ACHOWS_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFQ0048875.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVG
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18765..18821
                     /gene="ysgD"
                     /locus_tag="ACHOWS_003345"
     CDS             18765..18821
                     /gene="ysgD"
                     /locus_tag="ACHOWS_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFQ0048876.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18973..19923
                     /gene="corA"
                     /locus_tag="ACHOWS_003346"
     CDS             18973..19923
                     /gene="corA"
                     /locus_tag="ACHOWS_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFQ0048877.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19959..>20386)
                     /locus_tag="ACHOWS_003347"
     CDS             complement(19959..>20386)
                     /locus_tag="ACHOWS_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFQ0048878.1"
                     /translation="RPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQNEIKNQ
                     HPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINEY
                     PYRNTHFFIADNNEYQLTQENKIERVFNPHDFEKDNFQQ"
BASE COUNT         4772 a         5265 c         5286 g         5063 t
ORIGIN      
        1 ttagttttaa cggcaattaa tatatatgtt attaattgaa tgacttttat tattcattat
       61 atatatgtgt agaattgtgc gcaggagaaa tattcactca ggaagttatt actcaggaag
      121 caaagaggat tacagaatta tctcataaca agtgttaagg gatgttattt cccggttctc
      181 tgtggcataa taaacgagta gatgctcatt ccatctctta tgttcgcctt agtgcctcat
      241 aaactccgga atgacgcaga gccgtttacg gtgcttatcg tccactgaca gatgtcgctt
      301 ctgcctcatc agacaccatg gacacaacgt tgagtgaagc acccacttgt tgtcatacag
      361 acctgtttta acgcctgctc cgtaataaga gcaggcgttt ttttatgtat cagaaaggtc
      421 ccggaggagc ttgcctccgg gtgagaaaga gctactgtgg cgggttgttc tgcaacgtta
      481 acatcaaacc gtcgcgacgc atcgctgcgg cttcttccgg cttgtgcagt ctgtccagcg
      541 cgtcggcaag ccatgcgtaa tcgtaggcgt ccggacgttg tttcagcgct gcgcggaagg
      601 cgagcgatgc ttcctgccat tcgccgtgct tcatcagcga ctgacccagt gtgctccaca
      661 acagcgggcg atcgcccacg tttttgatct gttggcgcag cactttttca agctgttccg
      721 ggttattggt tttcagacgc gggatcggca gcagcaggcg atcgtcatac tggcgtttca
      781 ggccgtcgat gataatttgc tgggcagtat catgatcgtc acattcaata agatgttccg
      841 ccattgccac ctgtaacgcc acctgatgac gcgttttccg gctttggttt ttccaccagt
      901 tacgcaaacc ttcactaccg ttatcggcac gcgcctgatc catcaggcca atccatgcct
      961 gttgttccag cattgcacga tgttcttcat caccaacatg ggctttcgcc attgatggga
     1021 tgatatccag cagcgaactc catgcaccag tgcggatata cgcctgttcc gccagacgta
     1081 atacttccgg atggcgtggc gtaacttcca gcagcttatc aacgccgtga cgtgctgcat
     1141 ggttttcatt acgggccagt tgcagacgta cacgggtgat ttctaccgga atggtgtcat
     1201 tgccggccag ctccgctgcg cgttccagat gttggttggc gcgtgcttca tcaccacgtt
     1261 gttgcgcggc ttcggctgcc agtagatagt tcaccaccgg ttgttcagcg tgatcggcat
     1321 ttttcgccat cagcttttca acttgctgat aatcgccttc cgccagtttc agcagcgcct
     1381 gttcggtctg cttacgtgca cggcgacgct tacgtccgac aaaccaccca cgggtgtgcg
     1441 cgcctgtgcg gaagattcgc cgcagtagcc actcaatggc aaacagcact accatcgcca
     1501 gaatcaaaat aatcgcgagg cccgtgacgc tagtttcaat attgtagttg tcggtctgga
     1561 tcagcacgta accctgatgg ccggcaatca tcgggccaac cacgatcccc gcaatcagca
     1621 acacaaagag caataacact tttagcatgg ttattctcct tgcggcgcgg ctgccggagc
     1681 atcagcttgc ggtgcaggtg caggtgcagg tgcaggtgca ggtttagctt ccgttgtccc
     1741 cgctgccggt tgtgccagca ggttacgcac gcgagtttgc atcagttttt ccagcatcgc
     1801 ctggctttgc agggtttccg gaagatccat cgagatattt tgctggctta actggtccac
     1861 ctcgtcgagg aacgctttgg tggtggcatc atcagtatcg tagtaagcac gtacccaggt
     1921 ggagacgttc tccagcgcct ggcgataagt ctcttcctgg tgacgcggta cagcttgtgc
     1981 tgcgaccagc aggcgagagc gaatattttc gcgcagatag atatcctgat ttggcgctaa
     2041 cagcggtacg gcggtgtcat cacgacggcg aatcgtaatg aagttgtcca taaagttctg
     2101 ccagcttttt tgcagattga tacgccattc gctgatggaa ctggaaagct cttcaccgtc
     2161 ggaatccatc ggcgaaccat cgctgtcatt atcagccaga cgcagattat ctacctgatt
     2221 tgaaagctga ttaagcttaa ggatgatacc gtcataatcc acctgcgata ccgcagaaag
     2281 gctggcgata tcatcggtaa ttgcccgacg aacggtaatc agactcgggt cattcatatc
     2341 cgccaggctg gcatctgcac ttttcagcaa cgctgcagcg gtcgtgacgt cttgatcgct
     2401 ccacagcttc cgtcctgcga gtttcaccag gaaatcagcc tgagccagca gccaggtttt
     2461 agcatcgctg ccggaaatag tcgcgacctt ttgttggact tcatccagct gttttgccag
     2521 cgtttcttgc tgacgattcg cctgctcaag ctgtacagct tgttgcttga taatgccttc
     2581 cagctcagct ttttggctct cctgggcttt ttgcagtgcc gtaagttggt tagccagggc
     2641 atcgctggtg gcagtctgat tgacggcctg ttgtttaccc cagccataca aaccgacgcc
     2701 cgccgccaga gcaatagcga tagccaccgc gctgagaatc aatgcggtat tgttcttact
     2761 ctttttttct gttgcgacag gttgtgacgt ggtgtccacg gcctccctgg tctcttcaac
     2821 cacggcggag gttttttctt gttccgtcat tatggcttcc tgttatgaga gttattgtaa
     2881 tgcccgtaaa agcgcatcgt tgtcagcgtt atcggcgacc ttaatgtctt gccagcccag
     2941 ttcccgggcg agtttcgcca aacgctcact gacgaccaat agtcgacagc gtagtaacca
     3001 gtgctcacga taccattgtg ggatcagcga ccagagttgc tgcaacattt caccgctggt
     3061 aacaacgacc gtcgtcacct cgcgggattg ccagcgcatc gcttcttctg caccatcgta
     3121 atggattacg catcgttgat aacattcaca aaaagtgacc tcagcaccgc gcgccgtcag
     3181 ggtatcccca attagctcac gaccgccatt gccacgtaat atcagcgcac gtttgcccgc
     3241 aatattttgt aattcaggta attgtagcaa gacttcgctg atttcccgat cctgcgggta
     3301 gagaatcttc tgtccgctta cggtatgtag tgccagtgcg gtggtgcgtc caatggcgaa
     3361 ataagtaggt agtcggggcc atttacgatc ttgctgatgc agctgtgatt gggcaaaagc
     3421 aaccgcgtgt tgcgagaggg caaacaacag atcgctctcc cccagcgccg ccagttgatc
     3481 agcaagttgt ggtaattgtc gacccggaga aaactcaatc agtggaaaat gccaggccac
     3541 ctgccccagt gtgcgcagac ggctcactaa ctcttctcca gcgggagacg ggcgggtgac
     3601 aaggatactc atgccggggc gtctccatta tagacttcag cgaggatctc gcgcgcgccg
     3661 ttattcagta gctcttctgc cagcgaaatc cccatttgtt cggcatcttg cggcgcaccg
     3721 cggcgttcac cgcgaataat ctgcgaaccg tccggcgcgc cgaccagcgc acgcagccag
     3781 atttcaccat caataagctc ggcgtagcta ccaattggca cctgacatcc gccttcgaga
     3841 cgggtattca tggcgcgttc tgcggtaacg cgcagtgcag tttcgtggtg attcagcgcg
     3901 gcaagcagct cgcgagtgcg tgtatcatca aggcggcatt caataccgac cgcaccttgt
     3961 cctaccgccg gaagagaaat ctcgggtggt aacgcggcgc gaatacgtga ctccagacct
     4021 aaacgtttta gtccggctac ggcaagaatg atggcatcgt attcgccgtt atccagttta
     4081 ctcaggcgag tgccgacgtt gccgcgcagg gagcggataa tcagatccgg gcggcgttca
     4141 gccagttggc actggcgacg taaactggac gtcccgacga tactgcctgc cggtaacgca
     4201 tccagacttt catagttatt ggacacaaag gcatcgcgag gatcttcacg ctcgcaaatg
     4261 gtgaccagcc ccagaccttg cgggaattca accggcacat ctttcattga atgtacggcg
     4321 atatcggcgc gattctccag gagcgcgact tccagctctt taacaaataa gccttttccg
     4381 cctactttcg ccagcggcgt atcaagaatc acatcgccgc gcgtcaccat cggtaccagt
     4441 tcaacgacca ggcccggatg gctcgccatc aacttgtctt tgacatagtg tgcctgccag
     4501 agtgcaagtg ggctttggcg tgtggcaatt cttaaaacat tgtctaacat gcttgttacc
     4561 gtcattatca tccgtggtcc atcctaacat ccttgccaga gcgatgtcag tgttgtggtg
     4621 aaacgtagac gcctgcgcaa accgtaaaat gaggtctggc agtggatcct gacaggcgtt
     4681 tcacgccgtc gtaataagga atttacagag aataaacggt gctacacttg tatgtagcgc
     4741 atctttcttt acggtcaatc agcaaggtgt taaattgatc acgttttaga ccattttttc
     4801 gtcgtgaaac taaaaaaacc aggcgcgaaa agtggtaacg gttacctttg acatacgaaa
     4861 tatcccgaat gccgcgtgtt accgttgatg ttggcggaat cacagtcatg acgggtagca
     4921 aatcaggcga tacgtcttgt acctctatat tgagactctg aaacagagac tggatgccat
     4981 aaatcaattg cgtgtggatc gcgcgcttgc tgctatgggg cctgcattcc aacaggtcta
     5041 cagtctactg ccgacattgt tgcactatca ccatccgcta atgccgggtt accttgatgg
     5101 taacgttccc aaaggcattt gcctttacac gcctgatgaa actcaacgcc actacctgaa
     5161 cgagcttgaa ctgtatcgtg gaatgtcagt acaggatccg ccgaaaggtg agcttccaat
     5221 tactggtgta tacaccatgg gcagcacctc gtccgtaggg caaagttgtt cctctgacct
     5281 ggatatctgg gtctgtcatc aatcctggct cgatagcgaa gagcgccagt tactacaacg
     5341 taaatgcagc ctgctggaaa gctgggccgc ctcgctgggc gtggaagtta gcttcttcct
     5401 gattgatgaa aatcgcttcc gtcataatga aagcggcagc ctggggggcg aagattgtgg
     5461 ctccacccag catatactgc tgcttgacga attttaccgt accgccgtgc gtctcgctgg
     5521 taagcgtatt ctgtggaata tggtgccgtg cgacgaagaa gagcattacg acgactatgt
     5581 gatgacgctt tacgcgcagg gcgtgctgac gccaaatgaa tggctggatc tcggtggctt
     5641 aagctcgctt tctgctgaag agtactttgg tgccagcctt tggcagctct acaagagtat
     5701 cgattcccca tacaaagcgg tgctgaaaac actgctgctg gaagcctatt cctgggaata
     5761 cccgaaccca cgtctgctgg cgaaagatat caaacagcgt ttgcacgacg gcgagattgt
     5821 gtcgtttggc ctcgatcctt actgcatgat gctggagcgt gttactgaat acctgacggc
     5881 gattgaagat ttcactcgtc tggatttagt acgccgctgc ttctatttaa aagtgtgcga
     5941 aaaactcagc cgtgaacgcg cctgcgtagg ctggcgtcgt gaagtgctaa cccagttagt
     6001 gaaagagtgg gaatgggacg atgctcgtct ggcaatgctc gataaccgcg ctaactggaa
     6061 gattgatcag gtacgtgagg cgcacaacga gttgctcgac gcgatgatgc agagctaccg
     6121 taatctgatt cgctttgcgc gccgcaataa ccttagcgtc tccgccagcc cacaggatat
     6181 tggcgtactg acgcgtaaac tgtatgccgc gtttgaagcg ttaccgggta aagtgacgct
     6241 ggtaaacccg cagatttcgc ccgatctctc ggaaccgaat ctgaccttta tttatgtgcc
     6301 gccgggccgg gctaaccgtt caggttggta tctgtataac cgcgcgccaa atattgagtc
     6361 gatcatcagc catcagccgc tggaatataa ccgttacctg aataaactgg tggcgtgggc
     6421 atggtttaac ggcctgctga cctcgcgtac ccgcttgtat attaaaggca acggcattgt
     6481 cgatttgcct aagttgcagg agatggtcgc cgatgtgtca caccatttcc cgctgcgcct
     6541 gcctgcaccg acgccaaaag cgctctacag cccgtgtgaa attcgccatc tggcgattat
     6601 cgttaacctg gaatatgacc cgacagctgc gttctgcaat caggtggtgc attttgattt
     6661 ccgtaagctg gacgtcttca gctttggcga gaatcaaaat tgcctggtag gtagcgtgga
     6721 cctgctgtac cgcaactcgt ggaacgaagt gcgtacgctg cacttcaacg gcgagcagtc
     6781 gatgatcgaa gccctgaaaa ccattctcgg taaaatgcat caggacgccg caccgccaga
     6841 tagcgtggaa gtcttctgct acagccagca tctgcgcgga ttaatccgta ctcgcgtgca
     6901 gcaactggtt tctgagtgca ttgaactgcg tctttccagc acccgccagg aaaccgggcg
     6961 cttcaaggcg ctgcgcgttt ctggtcaaac ctggggctta ttcttcgaac gcctgaatgt
     7021 atcggtacag aaactagaaa atgccatcga gttttatggc gctatttcgc ataacaaact
     7081 gcacggcctg tcagtgcagg ttgaaaccaa tcacgtcaaa ttaccggcgg tggtggacgg
     7141 ctttgccagc gaagggatca tccagttctt cttcgaagaa acgcaagacg agaatggctt
     7201 taatatctac attctcgacg aaagcaaccg ggttgaggtg tatcaccact gcgaaggcag
     7261 caaagaggag ctggtgcgtg acgtcagtcg cttctactcg tcatcgcacg accgttttac
     7321 ctacggctca agcttcatca acttcaacct gccgcagttc tatcagattg tgaaggttga
     7381 tggtcgtgaa caggtgattc cgttccgcac caaatcgatt ggcaacatgc cgcctgccaa
     7441 tcaggatcac gacacgccgc tattacagca gtatttttcg tgatgagcgt gccggaaagc
     7501 gaggcttatc cggcatgcaa tcttagcgga aactgactgt ttcacccgct tgctgcgttg
     7561 ccgcctgttc cagcaaatcc cagaaggttt cgccgctacg atcgcaaatc cactcatcgc
     7621 ctttcagatc aaaatggtag ccgccctgtt tggttgccag ccatacctgg tgcagcggtt
     7681 cctggcggtt gataatgatt ttgctcccgt tttcaaaggt aatggtcagt acaccgccgt
     7741 tgatttcgca gtcgatatcg ctatcgccat cccagtcgtc cagacgctct tcaatggtca
     7801 gccacagttg atcagccagg cgatgaaatt cactgtcgtt cattgttgta tcctgttttt
     7861 aagtgatggc ggcagtatag cggcatgggg tcagagcttc aaagtttgct caatgtctgg
     7921 tgttttgtgg cactcttcct ccgtcgtcaa gatgatgatg tcatcattgg atgaaaacat
     7981 gttctgatcc agattacgtg tcctggccgg acagggcgat gcggttcgat aaagttggct
     8041 accgatccag gtataaacat agcgattgtc ataagcgaaa cgttcggtgg ttttcaatgg
     8101 tccatcaata gcaaaaattt gaatatcatc gcgtaaacgg gaagagcgca taaagtaacc
     8161 atggcctttc ccggcgttaa tccgttgttt caggaaaggt gcagaagagt cgagcgttat
     8221 tatttccagg gtgtcttcac caaaaggaga caatttcgca atgtacagat gatttttgta
     8281 ctgcgcgaca cgatcggtga tgggatgaaa tgctttcggg tcaatatcgg ggagtttctc
     8341 ccattgactc cattgctggt ctgtagagcg aagcttacgc cagctaacat cggtttcgcc
     8401 gacgagaaaa gagccttcgc catcagaata ttgagcaatg ttaacggatg agttataggg
     8461 gattcccgtt ggaataaacg caccatcctc aagaagtctg ccgtcaagct caccactacg
     8521 gtagtttgcc caatagccat atcctgaatc tttggttttc catttcaccc gcacggcttt
     8581 ggtattggga gttgggtagc gccaaagctg tgagccatcg aatgcgtaaa cataacgatc
     8641 gtcgtgtgca taccatttct tgtaatgggg gctatcgcta aggtgagcct catgctcgtc
     8701 aggaacacga taatccatca ggataagcgg cccggaagaa tcgaatacct ggaggccatc
     8761 ttcttcttca ccatcagctg gtaatttggt caataaatag ccgtgagttc ttcccgcgtt
     8821 aaagcgttta tctatcacca aaccaggatt atccagtgtg acgatattta gcctattttc
     8881 gccaaatccc gtttttttga tggtgtacag cttgtcctga tactgagcaa cggtattact
     8941 aagaggatga aattgttctg gatcgaggcc gggtaggact tcttgctggc attctggccc
     9001 tttgcgccat gaaacagagt gttcctgcaa agtgaacgcc gcgcagctat ttgccctatc
     9061 tttgtccaga tttccctgat tcagcacctt ccggtgcagg ccgcttttat ctcgccaggt
     9121 taataacgaa ccgggcatcc agcgtacgac agtaaatgtc tctgggtcgg ctttaagctg
     9181 ttcgccattg attatgattt tggtccgtgt acgggctaac gtatcccagg ggccaaatgg
     9241 tacggcaacg caggggttaa acgtggtgga aaactttcct cgctgatccc aggacttctg
     9301 cgccagcaca cggaaactct cggggtcagc gaggcgatgt ccatcgacat ataagaaatt
     9361 agcgtcacgt aatatcagcc ccagaaagtc aggtttccag ggggaacgga atttcacctg
     9421 atgcagcgtt ttaatatcta gactattttc tccgccattg tcatcggtgc gcgtgccttc
     9481 aaaatagaga ctgtgtttat ctgcggcaaa atcaccccat gccttgaacg aggcgacatc
     9541 gacgggaggt ttgtctggcg ggttttgcac aacttcaccc gcgtagagga tatagcgacc
     9601 atcgctatgc caggagtaat gaggatacca ggcctgtgcg ccttcataga ggtagtcggt
     9661 tgtcagatca gtcagaggag gtactttatc ccatttgtct agtgttgtcg ggtctattaa
     9721 taaattgggt aaacggcgca aacttttgta attcacgtca ggaagagaaa gatttgtttc
     9781 tttataaggg cgcaggtata ccacgccttt tccctgtttc tggtaaatat tatgttcagg
     9841 cgcggaaagc cgacatcccc atgccgacga taaaaacaac gaacaagaca acaaacaaac
     9901 cacattgcga aagtgcataa agccatcctg gcgcgaggtg ttgatcacga aactgctagc
     9961 agaacataaa tcctcacaag taagcgtaac tctcgcagca aatcgtcact tttcagaatc
    10021 cataaaggtc atcgctattt tctgatagtt gctggaataa acgtgatatt ttcattcacg
    10081 atataaacac aatcccggaa ctgccccata gtacggctaa tagcataaaa atattccgga
    10141 ttgacccctg gaagggaggc aatgtgggat tccgcccccc gttgccttct ttattgtctt
    10201 tcacgagttg tacccaggtt ccaccacggg tatctaacat tcgccacatt attacgcttt
    10261 ctgccgaacg tgatttttat agaaatctat caattcattt tccctgtacc ataaagttta
    10321 tccttcacat cctgccgaac gcgggtcttg taccccccgt tgtgaagatc ttcatacgga
    10381 gaaaaagaat ctttactcca ccccagcaac actttatcgc caggcataaa atgcacgtgc
    10441 aggtaatttt ctccccattc tctttttggg agaggtaacg tcatattgcg tttctccaga
    10501 cgcattccag cattatactg tgtacgagta gtacttaatg tccatataac ctctgccgta
    10561 tccccactga catcaccgca acaggtgatt gaaccgccac cattcgcaaa agcattcgcg
    10621 cccgccacgc cgttaacact gaatcccagt atcggacggt caatttcact gtggatttcc
    10681 agtatcactc cacctcttgg tcctccccat actgcctgtc ctatattcca caacacgacc
    10741 agtactggaa cgacaattaa cgtccgccat acccagcgcc cccagcgggc ataaccgcgc
    10801 gtaagctttt ggtcaaattt ttccagttgg gtaaatagcc ccatcgattt tattccctct
    10861 tctcacctgg ttggcaaagt tgcacgtcac tggcgactaa ggtcagacaa cgaaatttcg
    10921 tggtacgcca ctctcttaaa agtcccatgc aggcgataag aacaagacaa caaacaaacc
    10981 acattgcgat agtgcataaa gccatcctgg cgcgaggtgc cgatcacgaa actaccagca
    11041 aaacataaat cgccacgtgt aagcgttata ctcgcagcat ttcctcactt ttcagacttc
    11101 ataaagagtc gctaaacgct tgcttttacg ccttctcctg cgatgataga aagcagaaag
    11161 cgatgaactt tacaggcaat ccataatgaa aaacgtgttt aaggcactca ctgtattact
    11221 tactctcttc agcctgacgg gctgcggtct gaaaggtccg ctctatttcc cgcctgcaga
    11281 taaaaacgca ccgccgccga ccaaaccggt agagatgcaa acgcaatcca cggtgccgga
    11341 taaaaacgac cgcgccactg gcgatggtcc atcccaggtg aattactaaa agtcagtttc
    11401 tgtacccgcg tgattggagt aaatgatgca gttctcgaaa atgcatggcc ttggcaacga
    11461 ttttatggtc gtcgacgcgg taacgcagaa tgtctttttt tcaccggagc tgattcgtcg
    11521 cctggcggat cggcacctgg gggtagggtt tgaccagctg ctggtggttg agccgccgta
    11581 tgatcctgaa ctggattttc actatcgcat tttcaatgct gatggcagtg aagtggcgca
    11641 gtgcggcaac ggtgcgcgct gctttgcccg ttttgtgcgc ctgaaagggc tgactaataa
    11701 gcgcgatatc cgcgtcagca ccgccaacgg gcggatggtt ctgaccgtca ccgatgatga
    11761 tctggtccgc gtaaatatgg gcgaacccaa cttcgaacct tccgccgtgc cgtttcgcgc
    11821 taacaaagcg gaaaagacct atattatgcg cgccgccgag cagacaatct tatgcggcgt
    11881 ggtgtcgatg ggaaatccgc attgcgtgat tcaggtcgat gatgtcgata ccgcggcggt
    11941 agaaacgctt ggtcctgttc tggaaagcca cgaacgtttc ccggagcgcg ccaatatcgg
    12001 ttttatgcaa gtggttaagc gcgagcatat tcgtttacgc gtttatgagc gtggggcagg
    12061 agaaacccag gcctgcggca gcggcgcgtg tgcggcggtt gcagtaggga ttcagcaagg
    12121 tttgctggcc gaagaagtac gcgtggaact ccccggcggt cgtcttgata tcgcctggaa
    12181 aggtccgggt cacccgttat atatgactgg cccggcggta catgtctacg acggatttat
    12241 tcatctatga agcaaccagg ggaagaactg caggaaacac tcacggagct tgatgaccgg
    12301 gcggttgtcg attatctgat taaaaatcct gagtttttta tccgcaatgc gcgcgcagta
    12361 gaagcgatac gtgtgccgca tccggtacgc ggcaccgttt cgttggtcga gtggcacatg
    12421 gcccgcgcac gtaatcacat tcatgttctg gaagagaaca tggcgttgtt gatggagcag
    12481 gctatcgcca acgaaggcct gttttatcgc ttactctact tgcagcgtag tctcaccgcc
    12541 gccagcagtc tcgacgatat gctgatgcgc tttcaccgct gggcgcgcga tctcggcctg
    12601 gcgggtgcga gcctgcgcct gttcccggat tgctggcgtt taggtgcgcc gtcgaaccac
    12661 actcatctgg cattaagccg tcagtctttc gaaccgctgc gtattcagcg tttggggcag
    12721 gaacagcact atcttgggcc gcttaacgga ccggagctgc tggtggtgct accggaagcg
    12781 aaagcggtgg gatcggtggc gatgtcgatg ctgggaagcg atgctgattt gggtgtcgtg
    12841 ctgtttacca gtcgcgatgc cagtcactat caacaagggc agggaacgca gttacttcat
    12901 gaaattgcgc tgatgttgcc ggagcttctg gagcgttgga ttgaacgcgt atgaccgatt
    12961 tacacaccga tgtagaacgc tacctacgtt atctgagcgt ggagcgtcag cttagcccga
    13021 taaccctact taactatcag cgtcagcttg aggcgatcat caattttgcc agtgaaaacg
    13081 gcctgcaaag ctggcagcaa tgtgatgtga cgatggtgcg caatttcgct gtacgcagcc
    13141 gccgtaaagg gctgggagct gcaagtctgg cgttacggct ttctgcgcta cgtagctttt
    13201 ttgactggct ggtcagccag aacgaactca aagccaaccc ggcgaaaggt gtttcggcac
    13261 cgaaagcgcc gcgtcatctg ccgaaaaaca tcgacgtcga tgatatgaat cggctgctgg
    13321 atattgatat caacgatccc ctcgccgtgc gcgatcgggc aatgctggaa gtgatgtacg
    13381 gcgcgggtct gcgtctttcc gaactggtgg ggctcgatat caaacacctc gacctggagt
    13441 ccggcgaagt gtgggtgatg ggaaaaggca gcaaagagcg ccgcctgccg attggtcgca
    13501 acgctgtggc gtggattgag cactggcttg atttgcgcga cctgtttggt agcgaagacg
    13561 acgcgctttt tctgtcgaaa ctgggcaagc gtatctccgc gcgtaatgtg cagaaacgct
    13621 ttgccgaatg gggcatcaaa caagggctga ataatcacgt tcacccgcat aaattacgtc
    13681 actcgttcgc tacgcatatg ctggagtcga gcggcgatct tcgtggtgtg caggagctgc
    13741 tgggtcatgc caacctctcc accacgcaaa tctatactca tcttgatttt caacaccttg
    13801 cctcggtgta cgatgcggcg catccacgcg ccaaacgggg gaaataatgc gtttttaccg
    13861 gcctttgggg cgcatctcgg cgctcacctt tgacctggat gatacccttt acgataaccg
    13921 tccggtgatt ttgcgcaccg agcgagaggc gcttaccttt gtgcaaaatt atcatccggc
    13981 gctgcgcagc ttccagaatg aagatctgca acgcctgcgc caggcggtac gggaagcgga
    14041 acccgagatt tatcacgacg tgacgcgctg gcgttttcgt tcgattgaac aagccatgct
    14101 cgacgccggg ctgagtgccg aagaagccag tgcaggcgca cacgcagcaa tgatcaactt
    14161 tgccaaatgg cgcagccgaa tcgatgtccc gcagcaaacc cacgacacct taaaacagct
    14221 ggcgaagaaa tggccgctgg tggcgatcac caacggtaac gcccagccgg agctgtttgg
    14281 tttaggggat tattttgagt ttgtgttgcg cgctggcccg cacgggcgct caaaaccgtt
    14341 cagcgatatg tacttcctgg cggcggaaaa actcaacgtg ccgatcggcg agatcttaca
    14401 tgttggggac gatctcacca ctgacgtggg tggggcaatt cgcagcggaa tgcaggcctg
    14461 ttggatcaga ccggaaaatg gcgatctgat gcaaacctgg gacagccgtt tactgccgca
    14521 tctggaaatt tcccggttgg catctctgac ctcgctgata taatcagcaa atctgtatat
    14581 atacccagct ttttggcgga gggcgttgcg cttctccgcc caacctattt ttacgcggcg
    14641 gtgccaatgg acgtttctta cctgctcgac agccttaatg acaaacagcg cgaagcggtg
    14701 gccgcgccac gcagcaacct tctggtgctg gcgggcgcgg gcagtggtaa gacgcgcgta
    14761 ctggtgcatc gtatcgcctg gctgatgagc gtggaaaact gctcgccata ctcgattatg
    14821 gcggtgacgt ttaccaacaa agcggcggcg gagatgcgtc atcgtatcgg gcaactgatg
    14881 ggcaccagcc agggcggcat gtgggtcggc accttccacg ggctggcgca ccgtctgctg
    14941 cgtgcgcacc atatggacgc caatctgccg caggattttc agatcctcga cagcgaggac
    15001 cagctacgcc tgctcaaacg cctaatcaag gcgatgaacc tcgacgagaa gcagtggccg
    15061 ccgcgccagg caatgtggta catcaacagc cagaaagatg aaggcctgcg tccacatcat
    15121 attcaaagct acggtaatcc ggtggagcag acctggcaga aggtgtatca ggcgtatcag
    15181 gaagcgtgtg accgcgcggg cctggtggac ttcgccgagc tgctgctacg tgctcacgag
    15241 ttgtggctta acaagccgca tatccttcag cactatcgcg aacggtttac caatatcctg
    15301 gtggatgaat tccaggatac caacaacatt cagtatgcgt ggatccgcct gctggcgggc
    15361 gataccggca aagtgatgat cgttggcgat gacgaccagt caatctacgg ctggcgtggg
    15421 gcgcaggtcg agaatattca gcgtttcctc aatgatttcc ccggtgctga aactatccgt
    15481 ctggagcaga actaccgctc taccagcaat attctgagcg ccgctaacgc cctgattgaa
    15541 aacaataacg ggcgtctggg taaaaaactg tggaccgatg gcgcggacgg tgagcctatt
    15601 tccctctatt gcgcttttaa cgaactcgac gaagcgcgtt ttgtggttaa ccgtatcaag
    15661 acctggcagg acaatggcgg ggcgcttgcc gagtgcgcca ttctctaccg cagcaacgcc
    15721 cagtcgcgtg tactggaaga agcactgttg caggcgagta tgccgtaccg tatttacggc
    15781 gggatgcgct tcttcgaacg ccaggaaatc aaagatgcgc tctcgtatct gcgcctgatt
    15841 gccaaccgta atgacgacgc ggcgtttgag cgcgtggtga atacaccaac gcggggtatt
    15901 ggtgaccgga cgctggacgt ggtacgtcag acatcgcgcg atcgccagtt aacactctgg
    15961 caggcatgtc gtgaactgtt gcaggaaaaa gccctcgccg gacgtgctgc cagtgcctta
    16021 cagcggttta tggaactgat cgacgcctta gcgcaggaaa ctgccgatat gccgctgcat
    16081 gtacagactg accgggtaat taaagactcc ggcctgcgca caatgtacga gcaggagaaa
    16141 ggcgaaaaag gccagacgcg tatcgaaaac ttagaggaac tggtaacggc gacgcgccag
    16201 ttcagctaca acgaagaaga cgaagattta atgccgctgc aggcgttcct ctcccatgcg
    16261 gcactggaag cgggcgaggg gcaggcggat acctggcagg atgcggtgca gttgatgacg
    16321 ctacactcgg cgaaaggtct ggaattcccg caggtgttta tcgttggtat ggaagagggc
    16381 atgttcccaa gccagatgtc gctggatgaa ggcgggcgtc tggaagaaga acgccgtctg
    16441 gcctacgttg gcgtaacccg cgcgatgcag aaactgacgc tgacctacgc ggaaacccgc
    16501 cgtctatatg gcaaagaggt ttaccatcgc ccgtcgcgct ttatcggtga gttgccggaa
    16561 gagtgtgtgg aagaggtgcg cctgcgcgcc acggtaagcc gcccggtcag ccatcaacgg
    16621 atggggacgc cgatggtcga gaacgacagc ggctacaagc tcggccagcg cgtacgccac
    16681 gctaagtttg gtgaaggcac catcgtcaat atggaaggca gcggtgagca tagccgtttg
    16741 caggtggcgt tccagggcca gggtattaaa tggctggtgg cggcttacgc ccggttggag
    16801 acggtgtgac gttgcgggat gcggtgcgcc acaccgcatt ccgcatcagc accttactta
    16861 gcgcacttct tcacatcaat attccagtca gcaccagacg caacctgatg tttctgcgca
    16921 aaattatcca tattcagcgc cacggaaaca ttcaataagc tgttcagata caccatcggc
    16981 tggccttccg gcacatcgcc gaagcttgcg acatacggca ttttccctgc gtatttttgc
    17041 tgggaacctt cggaaatggt cacacacaac gtgtcgttga gtttgattcc tgcctgtttc
    17101 agcaactcgt cgctgatatt gctccagacg ttgccgtact gaatatcgag aatcgggata
    17161 ttgcccttca cttcaccttt tgttgctttt gctttctgat aagaaagttc gacaactttt
    17221 gcaggaagct ctggccccac ctgttcgaac gtaatcgctc cggaagccag acgcgcacca
    17281 gtgtaggcat atacatcgcg accatggaag gtatatgact tttcagaacc tttcaggcgg
    17341 ttagcttttt cgtcgatttc ccggacgcta tcaataccca gagtttgtgc caccaaagtg
    17401 agcgtaccgt tatctggtga aacgaagtat tgaccgtttt tggttttcag cactaccgat
    17461 ttacggtcag tgcctacgcc cggatcgacc acgctaacaa acaccgaacc ttgcggccag
    17521 taactggcag tctgatacag gcgataagcg ccttcccaga tgttatacgg tggaatttcg
    17581 tgtgttaaat caaagatttt aagattatgg tcaacgccga atgcgacccc tttcatcgcc
    17641 gagacggcac catctttcag gctaaaatcc gtttgtaaaa tcaaagcgtt atccgcataa
    17701 gcggatgatg accccagaca ggttaataaa aataatgttg cgagcgtttt tttcattttt
    17761 gtttcccttc atttatctga tgattttcca gctgtgtagc cagccccagt ttgtctggcc
    17821 tcctcgagtg tacaatgttt aacctttctg ctcgacggaa atcatggtga caaaaggata
    17881 gcgttgccac gggattgcct cgcctttcat atacatatgt gaaattgtgc catcaagata
    17941 aagcagctgc ccaacgttca gtttcgcttt ggcataacag gcaaaatcat aaaaatttgt
    18001 cgcctgctgg ctcaacaaaa acacggcgtt cccatgttta ttaatcccaa caccgttacg
    18061 aattttacgt gaggcgacgt tgggatgaat acgcggatta attacaccgt tttccatcaa
    18121 caatggccct gactgcaccg caaactgaat ctctttactg gttttaaagg catccagacg
    18181 aacgatgccg actttatctc ccgcgacata aaacactccg ccaggacgaa taaagaaatt
    18241 cccttcacct gaagcgagat ttaacgccac cttctgctga ccgttttcga tgtacaaacc
    18301 gagcggcgca tagctttcat catagatgcc gccgttcatc gccatctgca cctgaccctg
    18361 actattaata tccgccagca gagcatgtaa cgttccccac gcttcgccat tggctttttg
    18421 ccagtacatt ttcacccgct ctgtttgagg attaacggta tacgcctgta cggtcagcgt
    18481 cggatctgag ggtgcgcaat catcagcggc aacagcaaac aagggaagta aggtgagggt
    18541 gagaaaaatc cgtttgagat tcaaggtgat cattccttta ccaatgaata gccgatgcgc
    18601 cattataggc cctggatgtg ggattttttt atcctgttag cgaccttgac gagtaccaaa
    18661 aagcgcgaag ttcaactatt gttctgtggt gttctgttgc gtgttgacgg caaaattttg
    18721 ctggcgtaac atgcgcgcac gatcactcta agaggacatt cgccttggac acacccagta
    18781 gatactggct cactatcctg tcatccagga tcaactccta aggctatccc tttttgctga
    18841 tagccttagc ggttgtcagc gacctcaatt tttcccgtcg cgctgagtca ggctgtttaa
    18901 tggtctgaaa cccaatttgt ttctgtgtgc ccaccgaact gtccgatatt ttaagcattg
    18961 ggagtcccgg tcatgctgag cgcatttcaa ctggaaaata accgactgac ccggctggaa
    19021 gtcgaagagt cacaacccct tgtaaatgca gtatggattg atcttgtcga accggacgac
    19081 gacgagcgac tgcgcgtaca atctgaactt ggtcagagcc tggcaacccg cccggaactg
    19141 gaagacatcg aagcatcggc acgtttcttt gaagacgatg acggcctgca tattcactcc
    19201 ttcttcttct ttgaagatgc ggaagatcac gccggtaact ctactgtggc atttaccatc
    19261 cgtgatggtc gtctgtttac tctgcgtgag cgtgaactgc ctgcattccg tctgtatcgt
    19321 atgcgcgccc gtagccagtc aatggtggac ggtaacgcct acgagctgct gttggatctg
    19381 ttcgaaacca aaatcgaaca gttggcggat gaaattgaaa acatctacag cgatctggag
    19441 cagttgagcc gggtgattat ggaagggcat cagggcgatg agtacgacga ggcgctctcc
    19501 acgctggcgg aactggaaga tatcggctgg aaagttcgcc tgtgtctgat ggatacccag
    19561 cgtgcgctca acttcctggt acgtaaagca cgcttaccgg gtgggcaact ggagcaggcg
    19621 cgtgaaatcc tgcgagatat cgaatccctg ctgccgcata acgaatccct gttccagaag
    19681 gtgaacttcc tgatgcaggc ggcgatgggc tttatcaata tcgagcagaa ccgcatcatc
    19741 aaaatcttct cggtggtatc cgtggtgttc ctgccaccga cgctggtcgc ttccagctac
    19801 gggatgaact ttgagtttat gccggaactg aagtggagct tcggctaccc aggcgcgatt
    19861 atctttatga tcctcgcggg cctggcaccg tatctgtact ttaagcggaa gaactggttg
    19921 taatataaat gccgggtaaa gattgttctt tatccggctt attgctgaaa attgtctttt
    19981 tcaaagtcat gcgggttgaa cactcgctct atcttatttt cctgggttag ttgatactca
    20041 ttgttatcag caataaaaaa atgagtatta cggtaaggat attcattaat aaccattaaa
    20101 tagtttttta cgatgcaatt atcattgctc ttaatcgtcg ggaaacagta gagatcttca
    20161 tacggccctt cgatatattt tttatagtca gtgaagccat taccgatatc aataacatac
    20221 caggtatgcc aactaccttc tggtagggga tgttgatttt taatttcatt ctgatgcttc
    20281 agataccaat ttattttatc gctgtcagtc catggcagat gatcaacgac cagaaagact
    20341 gaattgccag catcagagta tgtatacaaa atctgtacag gacgca
//
LOCUS       DAZKJR010000091        20249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKJR010000091 DAZKJR010000000
VERSION     DAZKJR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.091"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..2214
                     /gene="crfC"
                     /locus_tag="ACHOWS_003348"
                     /pseudo
     CDS             1..2214
                     /gene="crfC"
                     /locus_tag="ACHOWS_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
     gene            2211..3089
                     /gene="rdcB"
                     /locus_tag="ACHOWS_003349"
     CDS             2211..3089
                     /gene="rdcB"
                     /locus_tag="ACHOWS_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFQ0048879.1"
                     /translation="MTKTLLDGPGRVLESIHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDAFRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            3353..4855
                     /gene="proP"
                     /locus_tag="ACHOWS_003350"
     CDS             3353..4855
                     /gene="proP"
                     /locus_tag="ACHOWS_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFQ0048880.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4967..5056
                     /gene="pmrR"
                     /locus_tag="ACHOWS_003351"
     CDS             4967..5056
                     /gene="pmrR"
                     /locus_tag="ACHOWS_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFQ0048881.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5032..6132)
                     /gene="pmrB"
                     /locus_tag="ACHOWS_003352"
     CDS             complement(5032..6132)
                     /gene="pmrB"
                     /locus_tag="ACHOWS_003352"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFQ0048882.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQNLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6133..6801)
                     /gene="basR"
                     /locus_tag="ACHOWS_003353"
     CDS             complement(6133..6801)
                     /gene="basR"
                     /locus_tag="ACHOWS_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFQ0048883.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6798..8441)
                     /gene="eptA"
                     /locus_tag="ACHOWS_003354"
     CDS             complement(6798..8441)
                     /gene="eptA"
                     /locus_tag="ACHOWS_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFQ0048884.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8545..9882)
                     /gene="adiC"
                     /locus_tag="ACHOWS_003355"
     CDS             complement(8545..9882)
                     /gene="adiC"
                     /locus_tag="ACHOWS_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFQ0048885.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10019..10780)
                     /gene="adiY"
                     /locus_tag="ACHOWS_003356"
     CDS             complement(10019..10780)
                     /gene="adiY"
                     /locus_tag="ACHOWS_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFQ0048886.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11116..13383)
                     /gene="adiA"
                     /locus_tag="ACHOWS_003357"
     CDS             complement(11116..13383)
                     /gene="adiA"
                     /locus_tag="ACHOWS_003357"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFQ0048887.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13582..14490)
                     /gene="melR"
                     /locus_tag="ACHOWS_003358"
     CDS             complement(13582..14490)
                     /gene="melR"
                     /locus_tag="ACHOWS_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFQ0048888.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14773..16128
                     /gene="melA"
                     /locus_tag="ACHOWS_003359"
     CDS             14773..16128
                     /gene="melA"
                     /locus_tag="ACHOWS_003359"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0048889.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(16125..16775)
                     /gene="yjdF"
                     /locus_tag="ACHOWS_003360"
     CDS             complement(16125..16775)
                     /gene="yjdF"
                     /locus_tag="ACHOWS_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFQ0048890.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(16898..18544)
                     /gene="fumB"
                     /locus_tag="ACHOWS_003361"
     CDS             complement(16898..18544)
                     /gene="fumB"
                     /locus_tag="ACHOWS_003361"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFQ0048891.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(18622..19962)
                     /gene="dcuB"
                     /locus_tag="ACHOWS_003362"
     CDS             complement(18622..19962)
                     /gene="dcuB"
                     /locus_tag="ACHOWS_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFQ0048892.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         4858 a         4990 c         5358 g         5043 t
ORIGIN      
        1 tgtatgagtt aagtcaggag gctgaacgcc tgttacagct ttctcgccaa cagttgcagt
       61 tactggaaaa aatacctctc tctgtacccg gagacgatac gccacaactg gctttaccct
      121 ggagtcagcc taatatcgcc gaacgtcacg cgatgctgaa taatgagttg cgtaaaattt
      181 cccggctgga aatggtgctg gcgattgtcg gtaccatgaa agcagggaaa tcaaccacca
      241 ttaatgccat tgttggtacg gaggttctgc ctaatcgtaa tcgcccaatg actgcgctgc
      301 caacgcttat tcgccatacg cccgggcaaa aagaaccggt actgcatttt tcacatgtcg
      361 cgccaatcga ccatttaatt caacaattac aacagcgcct gcgtgattgc gatattaagc
      421 atctgaccga tgtgctggaa atagataaag atatgcgtgc gcttatgcag cggattgaaa
      481 atggcatcgc tttcgaaaaa tattatctgg gtgctcaacc tatttttcat tgtctgaaaa
      541 gtttgaatga tttggtgcga ctggcgaagg cgctggacgt cgattttcct ttttctgctt
      601 acgccgccat tgagcatatt cccgtgattg aagtggagtt tgtccatctg gcggggctgg
      661 agagttatcc cggtcagttg acgttactgg atacccccgg gccaaatgaa gccgggcaac
      721 cgcatctgca aaaaatgctt aaccagcagc tggcacgcgc ctcggcggta ctggcggtgc
      781 tggattatac gcaactgaaa tcgatctccg atgaagaggt ccgtgaggca attttggcgg
      841 tggggcaatc ggtgccgttg tatgtgctgg tcaataagtt cgatcaacag gatcgtaaca
      901 gtgacgacgc cgaccaggtg cgggcactga tttccgggac gctgatgaaa ggttgtatta
      961 cgccacagca gatatttccg gtgtcgtcga tgtggggcta cctggcgaat cgggtgcgcc
     1021 atgagttagc caacaacggt aagttaccac cgccagagca acaacgctgg gtggaagatt
     1081 ttgcccatgc cgcgctcggc aggcgctggc gtcatgccga tctggcggac ctcgaacata
     1141 ttcgtcatgc tgccgatcag ttgtgggaag attcgctgtt cgcccagcca attcaggcgt
     1201 tgcttcatgc cgcttacgct aacgcctcgt tgtatgctct gcgatctgcc gcgcataaac
     1261 tgttgaatta cgcgcagcag gcgcgggaat acctggattt tcgtgcgcac gggttaaacg
     1321 tcgcttgtga acaattgcgg caaaatatcc atcagatcga agaaagtttg cagctattgc
     1381 aactcaatca ggcgcaggtg agcggcgaga ttaaacatga aatcgagctg gccctgacct
     1441 ccgccaacca ctttctgcgt caacagcaag atgcgctgaa tgcgcagtta gccgccttgt
     1501 ttcaggatga ttcagggtca ttaagcgaga ttcgtacctg ctgtgagaca ctgttacaga
     1561 tggcgcagaa caccattagt cgcgacttta cgctgcgttt tgccgagctg gaatccaccc
     1621 tttgcccggg tgttaaccga tgttattcgc cccattgagc aacaaatcaa aatggaattg
     1681 agcgagtcag ggtttcgtcc tgggtttcat tttcctgttt ttcacggcgt agttccccac
     1741 ttcaacactc gccagctgtt cagtgaagtc atttcgcgcc aggacgcaat ggacgagcag
     1801 agcacgcgtt taggcgttgt gcgtgagact ttttcgcgct ggttgaatca gcccgactgg
     1861 ggacggggaa atgagaaatc cccgatagag acggttgatt acagtgcgtt gcaacgagca
     1921 ttaagcgcag aagtcgatct ttattgccaa caaatggcta aagttctggc agagcaggtc
     1981 gatgaatctg ttacggcagg catgaatact tttttcgctg agttcgcttc atgtttgacg
     2041 gaattacaga cgcgtttacg cgaaagtctg gctctgcgtc aacaaaatga atcggtggtc
     2101 aggctgatgc agcagcaatt gcagcagact gtgatgactc atggctggat ttacaccgac
     2161 gctcagctgt tacgcgatga tattcaaaca cttttcacgg cagaacgata ttgaccaaga
     2221 cgttactgga cggccccggt cgcgtgctgg agtcgattca tccccgcttt ttagtggatc
     2281 tggcgcaggg cgatgatgcc cgccatccac aagcccatca gcagcagttt cgtgaacgac
     2341 tgatgcagga acttcttgcg cgtgtgcagc ttcaggcatg gacgaacggc gggatgttaa
     2401 atgcgccgct tagcctgcgt ctgacattgg tggaaaaact ggcgtcgatg ctggatcccg
     2461 gtcatctggc gctgacgcag atcgcgcaac atctggcgct gctgcaaaaa atggatcacc
     2521 gccagcactc tgctttccct gagctccccc agcaaattgc cgccttgtat gagtggtttt
     2581 cagcccgttg tcgctggaag gaaaaggcgt taacgcaacg aggcctactg gtgcaggcgg
     2641 gtgaacagag cgagcaaatt tttacccgct ggcgtgctgg ggcgtacaac gcctggtcgt
     2701 tgcctgggcg ctgttttatc gttctggagg agttgcgttg gggggcattt ggcgatgcct
     2761 ttcgtctggg aagcccgcaa gcggtggcgt tgttgctggg tgatttgcgc gtgaaagcga
     2821 cacaacatct ggcagagagt atcaatgcgg ccccgaccac gcgtcactat taccatcagt
     2881 ggtttgcctc ttcgaccatt ccgacgggcg gggatcatgc tgatttttta agttggctgg
     2941 gaaagtggtc cacggcagat aaacaacccg tttgctggtc agtgacccaa cgctggcgaa
     3001 ctgtcgcact ggggatgcca cgactctgtt cagcgcagcg tctggcgggg gcaatggtcg
     3061 aggaaatctt ctctgtaaat ttggtgtaaa taatcagtta catcaatgag tcctaaacga
     3121 aatccatgtg tgaagttgat cacaaattta aacgctggta gggtaaaaag gtcattaact
     3181 gcccaattca ggcgtcaact ggtttgattg tacattcctt aaccggagag tgtaagcaaa
     3241 cccgctacgc ttgttacaga gattgcatcc tgcaattccc gctccccttt tgcggccgtc
     3301 gcgctgattt ttctggcgtt tgcggaaatg ggccaactct gcgaggaaag ctatgctgaa
     3361 aaggaaaaaa gtaaaaccaa ttacccttcg tgatgtcacc attattgatg acggtaaact
     3421 gcgtaaagcc attaccgcag catcactggg taatgcaatg gaatggtttg attttggtgt
     3481 ttatggtttt gttgcttacg cattaggtaa agtttttttc ccgggggctg accccagcgt
     3541 gcagatggtt gctgcacttg ccactttctc cgttcccttt ctgattcgac cgcttggtgg
     3601 actcttcttt ggtatgttgg gcgataaata tggtcgccag aagatcctcg ctatcactat
     3661 tgtgattatg tcgatcagta cgttctgtat tggcttaata ccgtcctacg acacgattgg
     3721 tatttgggca ccgattctgc tgttgatctg taagatggca caaggtttct cggtcggcgg
     3781 tgaatatacc ggggcgtcga tatttgttgc ggaatactcc cctgaccgta aacgtggctt
     3841 tatgggcagc tggctggact tcggttctat tgccgggttt gtgctgggtg cgggcgtggt
     3901 ggtgttaatt tcgaccattg tcggcgaagc gaacttcctc gactggggct ggcgtattcc
     3961 gttctttatt gctctgccgt tagggattat cgggctttac ctgcgccatg cgctggaaga
     4021 gaccccggcg ttccagcagc atgttgataa actggagcag ggcgaccgcg aaggtttgca
     4081 ggatggcccg aaagtctcgt ttaaagagat tgccaccaaa cactggcgca gcctgttgac
     4141 atgtattggt ctggtaattg ccaccaacgt gacttactac atgttgctga cctatatgcc
     4201 gagttatttg tcgcataacc tgcattattc cgaagaccac ggggtgctga ttattatcgc
     4261 cattatgatc ggtatgctgt ttgtccagcc ggtgatgggc ttgctgagtg accgttttgg
     4321 ccgtcgtccg tttgtgctac ttggtagtgt tgcactgttt gtgttggcga tcccggcgtt
     4381 tattctgatt aacagtaacg tcatcggcct gatttttgcc gggttactga tgctggcggt
     4441 gatccttaac tgctttacgg gcgttatggc ttctaccttg ccagcgatgt ttccgacgca
     4501 tatccgttac agcgcgctgg cggcggcatt taatatttcg gtgctggttg ccggtctgac
     4561 gccaacactg gcggcctggc tggtcgaaag ctcgcagaat ctgatgatgc ctgcctatta
     4621 cctgatggta gtggcggtgg ttggtttaat caccggcgta accatgaaag agacggcaaa
     4681 tcgtccgttg aaaggtgcga caccggcggc gtcagatata caggaagcga aggaaattct
     4741 cgtcgagcat tacgataata tcgagcagaa aatcgatgat attgaccacg agattgccga
     4801 tttgcaggcg aaacgtactc gcctggtgca gcaacatccg cgaattgatg aataagctga
     4861 aacggatggc ctgatgtgac gctgtcttat caggccaatt gaactcttaa ggttcactta
     4921 atctctgacg cgcatactct cctccaggtt aacggaggag agtgcaatga aaaaccgtgt
     4981 ttatgaaagt ttaactaccg tgttcagcgt gctggtggtc agcagctttc tttatatctg
     5041 gtttgccacg ttctgatctt tcttcagccg tacccaggcc cgcgtgccgg aagtctcttg
     5101 ccggttttgc aggaaaaact gcccgtgatg caactgtgta atgcggctga caatacttaa
     5161 ccccagacca atcccgccat aacggctgtc catacgtaca aacgctttac tcaactcccc
     5221 gcatttactc tcatcaatac ccggtccttc atcttcaacg gccatgaccg ctccgccatc
     5281 ttcttgcagc ttaatcataa tgttgctgcc ttgcgggctg taacgatggg cgttttctac
     5341 caggtttcgc aataacatcc gcagcagggt tgcatcaccc tgaacggtga tgtcggcggc
     5401 gctctctggc aatagcaggt tttgctgtcg ctggtcgagc atggtactga gttcgtcata
     5461 cgaggggaga atgacatctt ccagcagttt tacatgttga taattaccgg aagaaaatga
     5521 ctgtccggca cgcgccagtt gcagcagctg ggagacactc tccatcatct gatcaagtcg
     5581 agccactaac ggtgctacat caatgtgatg cgttttcgcc agcagttcca gatgcaaacg
     5641 cacccccgcc agtggcgttc gcagttcgtg cgcgacgtca gcggtaaaca acctttcgtt
     5701 atccagcgtg ctggtcaggc gactgaccag atcgttcaac gccgaaacca ccgcttcgat
     5761 ttcgagggtg gcgctgtgaa tggcaatagg cgttaagttg tcggcggtgc gcgcttccag
     5821 ctctttttgc agctccgcca gcggccgggt gatgcggcgt accgcctgat agcagataaa
     5881 tagcgtcagg ctgaccataa agacgccggg gacgatcagg ctggcgaccg cctcgcggat
     5941 ctcacgcatg atgtggcgat cgttgttgcg attgtcgcgt agcgcctgct caaacagctg
     6001 aatctgctcg gtactttcat gccatagcca gaagacgctg atcagctcaa acaccaacaa
     6061 aatggcccca atggtcaata tcagccgttg gcgcagcgat attggtcggc gcagaaaacg
     6121 catcagattc aattagtttt cctcatttgc gaccagcata tagccaaagc cgcgcacggt
     6181 gcggatacgg gctttgccca ctttgtcgcg cagattgtgg atatgcactt ccagggtgtt
     6241 ggtcgagggt tcattgtccc agttatagat gtcgttgtag agaatttccc gatgcaccgg
     6301 actgcctgct ttgagcatta accgtgacag cagagcatat tctttgggcg tcagaatcaa
     6361 ctcttcaccg cccatccata cctgacggcg acccatattc agcgtcagat tgccaacaat
     6421 cagctcactt tcgccctgat tattatggcg tcgtagcagg gcgcggatac gggcgtgtaa
     6481 ctcttccagc gcaaaaggct tcaccagata gtcatcggca ccgacatcca gcccggcgat
     6541 tttgtcggtc agcgtatcgc gagcggtgag gatcagtacc ggcagggtgt attttttctg
     6601 ccggatacgg gcgagaaaat gcagtccatc ttcgtcgggt aaccctaaat ccagtaccac
     6661 caggctgtaa tgaccggcct caaggctttg ttccgccatc cgcgcggttg tcacgccatc
     6721 gcacgcgtag ccttcggttt gcgccgccag aattaatccc tgcaataaca gcgtatcgtc
     6781 ttcaacaatc agaattttca ttcactcact ctcctgcaag tttgcagaat atcatccgca
     6841 gcctggtaat acttcgtctc aacgccagtt aatcccaata gcgtggagaa taaattatct
     6901 tgtgaatagt gttgcgtttg cgcctgtttt tgcaggcagt tctggtcaac ctgataccgt
     6961 ttttgataat cctccgacag ccacagcagc atcggcacct gtttttggct atccggggcg
     7021 atggcataag gcagaccgtg cagatagatg ccattttcac ctaacgactc accgtggtca
     7081 gaaagataaa ccaggctggt ggtaaattta tcctgatgtt ctttcagcag attaatcgct
     7141 ttatcaacaa tatagtcgac gtaaaccagc gtgttgtcgt aagtgttcac cagttgctct
     7201 ttggtacagg tctggatctc gttagtgtca caggttgggg taaatttcct gaactgcggc
     7261 ggatagcggt tgtaataggt cggaccgtgg ctgccgatgg tgtgtaagac aatcacgcca
     7321 tcaccctgca ggttattgat gtactcttcc agcccgtgga acagcacttc gtcatagcat
     7381 tcgccgttga tgcactgacc tggcagattc agcgcggtga cgttctggtg aggtacgcga
     7441 tcgcaaacgc ctttacagcc gccatcgttg tcattccaca gcacgttgat gcccgctcgc
     7501 tgaatgatat ccagcacgcc ttcctggtgc tgtgccagct cttctttgta gtgctcacgc
     7561 ggcatatccg agaacatgca tggtactgaa actgccgttg ccgtgccgca agaggcggta
     7621 ttagggaaat agaccacgtt atcttttgcc agacgaggat tggtttcgcg tggataacca
     7681 ttcagcgaga aattctctgc gcgcgacgtt tctccgacga tcaaaatggt caggttttta
     7741 cgtttcccat tttgcattaa tgggttgcgg tgagcatctt caccaatgcg tatcagtggc
     7801 aggttggctg accgctggtg agagtaccac gaccagctag caacaatgct gttggacggg
     7861 cttaaggatt tcaccagctc tttgttgttg cgaaacagcg aggcatagtc tttgtagaac
     7921 agtgcggcaa ccagcagaat cagcagtaca gaaatcagga tgttggcccc gcggaaaaga
     7981 acgctgcgca ggcgtgaggt ggtgggggtg attttgatcc agcaggcaat aatcgcggct
     8041 aacacgccac ttaaccccag cgttaataac attcgtggcg tcatcagggc ataactttcc
     8101 gccggcgtag tatcaatgat attggtgatc attgaacggt cgatgacgat gccataggtc
     8161 atgatgaaat attgtgcagc cgcaccgacc agaataaaca ggcaggcgag tggacgatta
     8221 agccataaga aagagcctag tgtcaggaca atattaatca cgctgaatgc gacgaccggc
     8281 atcgacaaga aaaccagcac gttatgcagt gaatccagcg gtaacacctg tagcacctgt
     8341 ttaaaaaagg cgatattcag gcagactgag atataaaaag tagcaagtaa taaccaggag
     8401 aataaattta aagagggtct ttttagtagg cgcttcaaca tcacggtgtt tccatcgaac
     8461 aaagtgcgca tatgctcgca aagcaaaatt aagccaacct taagttctta aggttggctt
     8521 ttatgtttgc ttgattaagg ggaattaatc tttgcttatt ggtgcatcta agggatacgg
     8581 gtttttatgc agccggttgt aattcagcgc atacatggcg gtgattacca tcagagtgac
     8641 aaatgaccac ataacctctt tcgcgccgga ccctaccacg gcccagatgc agtagaggaa
     8701 ggcaatggta gtcactgcca ggtatgccgg gcgtgcttta ccaaagtgac cgtggccgag
     8761 cagtagtaac gccgcacagg tgtaaagata tggcaccagt gtaaagatga ccgacacgga
     8821 agaaaccaga ccgaattctt tggtcgcgtt tggcgaaatg ctactgagct ggaagatggt
     8881 catcaaaata ccgacgataa tcagccccgc cactggcgtg cccgctttat taacacgggc
     8941 aaaaatcggt gggaacagtc cgtcatcggc agcggctttc gccgtttgac ccgccagcaa
     9001 cgtccagccg cccagtgaac ctaagcaacc cgcagctgcg cagaaggaaa caatggcccc
     9061 ggcggtgtca cccaacgcca tccgtgcggc atcaccgaat ggcgaagcag aaacgcgcag
     9121 tgcggcatta gggatcatcc ccataatcgc ggtggtagaa agtacatagc aaacggcagc
     9181 aatcaatacc ccgccaatgg tggcgatagg gacgttgcgt ttcgggtttt tcaccacacc
     9241 tgcggcaacg gaggcacttt ccacaccgat gaacgaccac agcgtaacgt taagggtact
     9301 ttgaatcgca ccgaaggtgc ccagaccgct gacgttccat gccgccatat aggtttcacc
     9361 acggaaccag aaccagccaa atacggcaat cccgacgatg gggatcagcg ccagcacggt
     9421 agcaacggcc tgtacgcggg tgatcatttt cggaccgaca atgttcagca ggacgaagat
     9481 ccacagcacc acgacgcagg tgatggttaa taccaatggg tctttcagaa tcgggaagaa
     9541 gtaacttaaa tatcctacgc caatgaccac catggcgata ttgccgatcc agcaggccag
     9601 ccagtagagg acgttggttt gataaccgag aaacgggcca aagcagcggc gggcgtaagc
     9661 gtaagaacca ccaggacttg ggtcgaggaa cgacattttg gcgtatacca tcgagagccc
     9721 cagcgcaccg ataatcgtca ccaaccatcc ataaatggca atcccgccag tggaggccag
     9781 gtttgcaggt aacagaaaaa cacctgaccc cataatattc cccgacacca tcagggtgac
     9841 ggggattaag cccactttgt gagcatcagc atccgaagac ataattaaac tcctgcgaag
     9901 gcgagcttcg tgacaataaa ttacgtcgta tcatacgcct gcattcgata gttgaattat
     9961 tatcggccgg gttatcgctg atgccgttat taatatagct cttcgttaag gttaaaaatc
    10021 aggcgacagt gcgttctctg tgctgactaa cataatgcag cggcgtcata ccgtagaagt
    10081 ctttaaagac agaaataaag tacgacgtac tgttgtagcc gcaggactgt gatacctgag
    10141 agatattttt accgtccatc attaattcat ttacggcata gcgcatgcgg caggtggtga
    10201 ttatttggct ataactggtg ttttcgcttt tcagcttttt ctttaacaga cttgggctaa
    10261 gacataaaca actggctacc atactaagat tccagtcttt gtggatatcg ctttcaataa
    10321 tttgataaac gctgtcactt acacaattac gtaacatata catcattagt gaaaggaatt
    10381 ttttactgtc gagaaaacgg gatagcacag tgaataataa tgcacgtgtt cgttcctttt
    10441 ctgactcagt ttcagcgggc atcatgctat gttgcgcggc aacgcgaaag acgtctggcg
    10501 tcaggcatgg aagggtgagg atgggcgtag cgctacgttg ccatactggt atttgcgaga
    10561 gatctttatt cagaaaccgg aggtaatctt tgatgatgtc gtgactaata tgagcaacca
    10621 aagtgttatt aagagaggag acgtcgataa tattattttc acaatttaat aacgccatat
    10681 ggttagcttt aaggctaata ggttctttcc cattcaccct tatccagaca tctttttcag
    10741 tcaataaaac aatacaaggt tggtcgctgc aaatcctcat acatgtactc ctgagtgcga
    10801 ataaaagacc agttctcgct aaagcaaagc gataccggga aatggttata taattgtatg
    10861 atgaatataa acatgcataa aaataacgag ttttactaat attacaaatt tttatagtaa
    10921 tttctcatgt acatattttt ttacaggcta aaaaaaggat aaataacagg caaaaaaaag
    10981 cgcggggtgg gaccgcgcaa aaagcatgac attttttgtt tgcagcaatt tttatgaaaa
    11041 cgttgccata cttaaaaggc ctcaagccag gcggcgtaat gttatttaac aattacgcct
    11101 tcagcggaat agtggttacg ctttcacaca cataacgtgg taaataccgt caataatttc
    11161 agtcccttca gtttcgtgtt caaatccagg gaaatgatgg tcccaggatt gcagcgagcg
    11221 taaataactt acttgcggac tgtttttatc gccgaagttt tcaccagaca gcagcatcgg
    11281 gattcctggc ggatacggga taactgagtt tgccgcgatg cgtcctggta ggttttcaat
    11341 ggataccagt tcgacattgt tgtcgacaat cgcgttgtac gcttctcgcg gggtgatttc
    11401 cgccaccggc aggccggaat aggcttcgtt caaccgtgcg ccagggttgt tttctttcag
    11461 ccaggcaaac atggtgtcac ccagatcgtg aatccccatg ttcgcgtaag tgtcaggata
    11521 ttgttcaaca agttccggca tcacctgcgc cagcggggtg ttggcgtcat agtggcgttt
    11581 gaaggagcaa agggtgttaa ccagagtccc ccatttccca cgggtcacgc ccatagagaa
    11641 caggaacata atctggaagt cagtggtacg ggtaggcaca atgccgtggc gaccaagcca
    11701 ggctgtgacc agcgccgccg gaacaccggt ttcttccagt tcaccatctt cacccattcc
    11761 cggagcaagg atgctgactt taatcgggtc gagcatactc cagttatccg gaatatcttt
    11821 gaagccgtgc cagctttcgc ccggatgcat gacccagcag tcctgaacgg tggtcagcag
    11881 tttggttggt gcgtcagcaa agtcataggt tttgccggtt tgtgggtcgg tgacgacttc
    11941 tttgttccac ggtttgaaga accagctacc gtcagcggtg aactctttat acagccgcgc
    12001 catcgcctga cggaaatcaa ccgcttcgtc gatcacttcc tgcgtcagtg acaggccgct
    12061 gttgccgtcc atcatcgaca ccgccacgtc gttggatgcg cagatggcat acagcgggga
    12121 ggtggtggca tgcatcatgt aggcctggtt gaagcgggag aagttaatcg ccccacgacc
    12181 ttcacgtaca tgaatataag aggcctgtga cagcgcattc agcagtttgt gggtggagtg
    12241 ggtggcgaaa acggtagggc cgttgtgatc gccaggttcg ccgcgcatgg catagtgatc
    12301 ggcatagatt gggttgaaac gtgcatagcc gtaccaggct tcgtcaaagt gcagacgatc
    12361 ggaggttttt tccagcagat cctgcgcttc tttagcgtta taacacacgc cgtcataggt
    12421 acagttggtc accacgcagt aagacggttt ttgcccggct ttgtctttgg tcagcgggct
    12481 ttcactgatt ttcttctgca aggtttcagg ttgcatttcc tgcggataga ttggcccgat
    12541 aatgccgtag cggttgcggc ttggcaccat gtagaccggt ttcgcgcctg tcagcatcaa
    12601 gccttgttcg atggatttat ggcagttacg gtcaacgacc acgacatcgt tatcggtcat
    12661 gcaagcctgc atgatggtgc ggttagagcc ggaagtaccg acgactaccg accaggagcg
    12721 atcggcacca aatacgcgtg cagcatattt ttcgctttcg ccaaatgcgc cagtatggtc
    12781 aagcaaagaa ccgagggaag ttcgttcgat gcccatgtcg gtgcggaaca gattttcacc
    12841 atagtagtca tggtagaaac gtccggcggg tgttttggta aaaccaacgc cgccctggtg
    12901 gcctggcgct gcccaggaat attcatggat gtcgctatat ttcatcagcg cgctgaacag
    12961 tggcggcaac agttgctggc ggtagcgggt catcgcggca acggcgcgtc cggcgataaa
    13021 gtcggcggta tcttccagaa tccaggcgaa ttcatcgaca agctccagca gatcccggtc
    13081 cattgcggcg agggcttttt cccgatcgcc caacaggaag accggcacgt tttgttgacg
    13141 ctcatgaagc ttaccgatca attgtctgac gttttgatgt tcgtccggat gttccatttg
    13201 atagctgaac atcaggcagt caatggcttc gtttgaagag agaatggcaa aaccatcatc
    13261 aaaggaggtg gatttaatca cggtaacatt ttgctggctt aaagcatctg ccagacgctc
    13321 aacggcgtta ccgacccagg tgtcttgatg gagaaactcg ctttcaacaa ttaatacttt
    13381 catcattgct tacccggtta tgaaggaatc ttcgttgcgg gcaagtatct tccggccttt
    13441 tcgggctgta aagcgcgtga aaaacaaaca tttttgaaat atttgttttt ataaacaata
    13501 tgttatgtga ttattttaaa ttattttcac tgcttattat cgacggctaa actatttttt
    13561 tggctgatac tgatatcgtc tttagccggg aaacgtctgg cggcgctgtt ggctaagttt
    13621 gcggtattgt tgcggtgaca tgccgacata tttgccgaac gtgctgtaaa aacggctact
    13681 cgaacgaaag cctgccgtca gggcaatatc gagaatactt ttatcggtat cgctcagtaa
    13741 cgcgcgaacg tggttgatgc gcatcgcggt aatgtactgt ttcatcgtca attgcatgac
    13801 ccgctggaat atccccattg catagttggc gttaagtttg acgtgctcag ccacatcgtt
    13861 gatggtcagt gcctgatcat agttttcggc aataaagccc agcatctggc tgacataaaa
    13921 ttgcgcatgg cgcgagacgc tgtttttgtg tgtgcgcgag gttttattga ccagaatcgg
    13981 ttcccagcca gagaggctaa atcgcttgag catcaggcca atttcatcaa tggcgagctg
    14041 gcgaatttgc tcgttcggac tgttcaattc ctgctgccag cggcgcactt caaacgggct
    14101 aagttgctgt gtcgccagtg atttgatcac catgccgtga gtaacgtggt taatcaggtc
    14161 tttatccagc ggccaggaga gaaacagatg catcggcaga ttaaaaatcg ccatgctctg
    14221 acaggttccg gtatctgtta gttggtgcgg tgtacaggcc cagaacagcg tgatatgacc
    14281 ctgattgata ttcacttttt cattgttgat caggtattcc acatcgccat cgaaaggcac
    14341 attcacttcg acctgaccat gccagtggct ggtgggcatg atatgcggtg cgcgaaactc
    14401 aatctccatc cgctggtatt ccgaatatag cgacagcggg ctgcgggtct gtttttcgtc
    14461 gctgctgcac ataaacgtat ctgtattcat ggatggctct ctttcctgga atatcagaat
    14521 tatggcagga gtgagggagg atgactgcga gtgggagcac ggttttcacc ctcttcccac
    14581 agcggcgagg ggagcgtccg agcatcatga ggccgaaaac tctgcttttc aggtaactta
    14641 ttcccataaa ctcagattta ctgctgcttc acgcaggatc tgagtttatg ggaatgctca
    14701 acctggaaga cggaggtttt ctgcagattc gcctgccatg atgaagttat tcaagcaagc
    14761 caggagatct gcatgatgtc tgcacccaaa attacattta tcggcgctgg ttcgacgatt
    14821 ttcgttaaaa atattcttgg tgatgtgttc catcgcgagg cgctgaaaac ggcgcatatt
    14881 gccctgatgg acattgatcc cacccgcctg gaagagtcgc atattgtggt gcgcaagctg
    14941 atggattcag caggggccag cggcaaaatc acctgccaca cccaacagaa agaagcctta
    15001 caggatgccg attttgtcgt ggtggcattt cagattggcg gttatgaacc ttgcacggtg
    15061 actgatttcg aggtctgtaa gcggcatggt ctggaacaaa ccattgccga tacgttgggg
    15121 ccgggcggta ttatgcgcgc gctacgtacc attccgcatc tgtggcaaat ttgcgaggac
    15181 atgacggaag tctgccccga tgccaccatg ctcaactacg ttaacccaat ggcgatgaat
    15241 acctgggcga tgtatgcccg ctatccgcat atcaaacagg tagggctgtg ccattcggtg
    15301 cagggaacgg cggaagagct ggcgcgtgac ctcaatatcg acccggctac gctgcgttac
    15361 cgttgcgcag gtatcaacca tatggcgttt tacctggaac tggagcgcaa aaccgccgac
    15421 ggcagttacg tgaatctcta cccggaactg ctggcggctt atgaagcagg gcaagcaccg
    15481 aagccgaata ttcatggcaa tactcgctgc cagaatattg tgcgctacga aatgttcaaa
    15541 aagctgggct acttcgtcac ggaatcgtca gaacattttg ctgagtacac accgtggttt
    15601 attaagccag gtcgtgagga tttgattgag cgttataaag taccgctgga tgagtacccg
    15661 aaacgctgcg tcgagcagct ggcgaactgg cataaagagc tggaggagta taaaaacgcc
    15721 tcccggattg atattaaacc gtcacgggaa tatgccagca caatcatgaa cgctatctgg
    15781 actggcgagc cgagtgtgat ttacggcaac gtccgtaacg atggattgat tgataacctg
    15841 ccacaaggat gttgcgtgga agtagcctgt ctggttgatg ctaatggcat tcagccgacc
    15901 aaagtcggta cgttaccttc gcatctggcc gccctgatgc aaaccaacat caacgtacag
    15961 acgctgctga ccgaagccat tcttacggaa aatcgcgacc gtgtttacca cgctgcgatg
    16021 atggacccgc atactgccgc cgtgctgggc attgatgaaa tatatgctct tgttgacgac
    16081 ctgattgccg cccacggcga ctggctgcca ggctggttgc accgttaagg ggtaatcgct
    16141 tccggcgctc tggtgattaa gccaaagcgc cgtagctggc ggcaatgaaa gcgagcgagg
    16201 aatatcaccg tcgttaatgc gccaagcagc gcgcagaaca tatcggattg cgtgtcccac
    16261 tggtcgccct gggtgccgag aaaatcatcc gctccctgac ccattgccag cgccgcccac
    16321 cactcgatta attcatacat cgcgcttatc gccagcgcca cgcagcagac cagaaacgcc
    16381 accatcttac gtccgcgaac gtacatcccg cgtacaagga tctctcgtgc caccagtgca
    16441 ggcaccagcc cctggaaaaa atgccccagt ttgtcatacg gattacggct caaccccagc
    16501 cattcctgca cctcaaaacc aacggggact ttcgcgtagg tgtattgccc gccgaccatc
    16561 aggatgatgg cgtgaaagaa aatgagcgta taaagcagcg gagttaacgg ataacgttta
    16621 gcggtggcaa gcagtagcgg cacgacaata atcaccggtg tcacttccat cagccaggtg
    16681 agtttgtcat gggccgaaat gccggtataa atcaggatta acgttagcgc cagtgcgccg
    16741 gtgttaagaa ttaacggctt gagtgtgcgg gtcatggtga gttcgcaagt cagggaaaat
    16801 actgactatt gaccagtaag cgtgaaatta caatcgcaaa agaggtggcc cagggggatc
    16861 acctggcagc atgctgccag gcgctgggcc gaagaggtta cttagtgcag ttcgcgcact
    16921 gtttgttgac gatttgctgg aagaagtcgt tgcctttgtc atcgaccagg ataaacgccg
    16981 ggaaatcttc tacttcgatt ttccagatag cttccatacc cagctccgga taagcgacgc
    17041 attccagatg cttgatgctc tgctgcgcca gtaccgccgc cggaccgccg atactaccga
    17101 ggtagaagcc gccgtgttta tgacacgcgt cggtaacctg ctgactgcgg ttacctttcg
    17161 ccagcatgat catgctgccg ccgtgggatt gcagcagatc cacgtaggag tccatacgac
    17221 ctgcggtggt tgggccaagt gaacctgatg gataaccggc aggggttttc gccggacccg
    17281 cgtagtagat cgggtgatct ttgatgtact gcggcagttc tttaccggcg tcaatcagct
    17341 ctttcagctt ggcgtgtgca atatctcgac ccacaataat ggtgccggtg agcgacaaac
    17401 gggtggatac cgggtattgc gaaagctggg cgaggatctc tttcatcggg cggttaaggt
    17461 caactttcac cgcttcgcct tcaccggcct ggcgcagttc ttgtggaatg tactggcctg
    17521 ggttgtgttc cagtttttcg atccagatac cttcgcggtt gattttcgct ttaatgttac
    17581 ggtctgcgga gcaggagacg cccatgccta ccgggcagga tgcgccgtga cgtggcagac
    17641 ggataacgcg aatgtcgtgc gcgaagtatt taccgccaaa ctgcgcgcca agaccgagtt
    17701 tctgggcctc ttccagcagt tcctgttcca gctggacatc gcggaacgcc tgaccatgtt
    17761 cgttcccttc cgtcggcagt tcatcgtaat agtgagcgct tgctaacttg acggttttca
    17821 ggttggtttc cgcagacgta ccgccaatca caaacgcgat atggtacggc gggcaagctg
    17881 ccgtaccgag ggtacgcatt ttctcgacga ggaagttttt cagtttgccg ggcgtcagca
    17941 gggctttggt ttcctggtag agatacgttt tgttggcaga gccgccgcct ttagcgacac
    18001 agaggaattt gtactcatcg ccatctaccg cgtacaggtc gatttgcgca ggcaggttcg
    18061 tgccggtgtt gacctcttta tacatgtcca gcggcgcgtt ctgtgaatag cgcaggttat
    18121 cttcgatata ggtgttatag acgcctttcg acagcgcttc ttcatcaccg ccaccggtcc
    18181 acacgcgctg acctttttta ccgacgatga tcgcggtgcc ggtatcctgg caggtcggca
    18241 gcacgccttt ggcggcgatt tcggagtttc ttaagaattg cagcgccacg tacttgtcgt
    18301 tttcgctggc ttctggatcg tgaagaatag ccgcaacctg tttctggtgt gctgggcgga
    18361 gcatgaaaga agcgtcgtga aaggcttgct gcgccagcag ggtcagggct tctggttcca
    18421 ctttcaggat ggtttcgccg tcgaagtcgg caacgctaac gtaatcggaa gtgagtagat
    18481 agtattcggt attgtctttc cccatcggga aaggtgcctg gtagataaag ggtttgtttg
    18541 acatagcttc cagcctgtaa ctctgtattt gttattcgaa aatggcgtgc cgcgtagtca
    18601 cgggcggcac gcaaagtgca tttataagaa cccgtacatc gcggcgaaga tccagccgaa
    18661 gacgcacgat acgctcacac caatcaaccc cggcagaata aagctgtggt tgatgacgaa
    18721 gcgaccgatg tgggtggtgc cggaacggtc aaactgaatc gctgccagat cgctcggata
    18781 agtcggcagg atgtaataac cgtagcaagc cggtgctgaa gccacgatgt atgccggatc
    18841 aacgccgatc gccagcgcga ccggaacaat cgccgccagc gccgcagcct gagagtttac
    18901 aaacttggaa accagcagca gaacaatggc ataggcccac ggatactctt tcaccatttc
    18961 acccagtacg ccctgaattt cagacatatg cgcaccgaac atggtttctg ccatccatgc
    19021 gataccgtac accgccacga tggcgatcat accggaacgg aagacttcgt tttttgagat
    19081 agacgcggga ttggttttgg tcaggataat aatcagcgcc ccggtcagca gcataaacat
    19141 ctgaataacc agtaccatcg acagcggttt gccgccgaag gatggacgca ggtccgaatc
    19201 agcaccaaga agggcgacta cagcgattgc cccgaggaag atccacattg ccagccagtt
    19261 gcttttcggc agttttttat ccagcagcgt cgcggtatca ccgtaaacat actcacggtt
    19321 ttccggtacg gagatgaatt tctggaactc ttcgtcttta tccagatctt taccgcggaa
    19381 ccagctgaag ataccgatcg ccaggatacc gattaacgtc gatggaatgg tgattgccag
    19441 caggtcgagg aactcaagat ggcgaccatc aaaggtgaca ttacccagca tcgcaaccag
    19501 agacacgact gcaaccgaca ccggactggc gataatcccc atctgtgcac cgatagaact
    19561 tgccgccatt ggacgttccg gacggatgtt gttcttaata gcaacgtcgt agatgatcgg
    19621 cagaatggtg taaaccacat gacccgtacc gcaaagaatg gtcagcgtac aggtcacaaa
    19681 cggcgcgaca attgagacat atttcgggtt gcggcgcagc agcttctcgg caatttgcag
    19741 catgacatca agaccgcccg aagcttgcaa ggtcgccgat gccgccacca ccgcaatgat
    19801 aaccagcatg acatcaaccg gtggtttacc tggctgaagg tggaagacga agaccagaat
    19861 gaccagaccg ataccgccta ataaacccag cgcgatacca ccctttctgg caccataaaa
    19921 cagacatatc agtattatga taagttggat agtaaataac atgtgtgaac cctcgcgata
    19981 atcctattta aatttttgct gaatagatca cagtcacgtt ctgttttgta tgaactgttt
    20041 tcagagcctg actaaatatc cgtttggtcg ttaccggctt tagcaaatac ctcacagtga
    20101 atattggctg attaaagcag cggcaaattt atgcactgtg ataagcggct ttttcagaga
    20161 gaaaaactct ctggcgatgg gtttatagtt tgtactgcgc agatgttaat gttttgtttg
    20221 ctgttatctt attgatattt atcggtcta
//
LOCUS       DAZKJR010000092        20208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKJR010000092 DAZKJR010000000
VERSION     DAZKJR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.092"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..490
                     /locus_tag="ACHOWS_003363"
     CDS             <1..490
                     /locus_tag="ACHOWS_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0048893.1"
                     /translation="RKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTM
                     QLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVM
                     RMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS
                     VA"
     gene            751..1092
                     /gene="raiA"
                     /locus_tag="ACHOWS_003364"
     CDS             751..1092
                     /gene="raiA"
                     /locus_tag="ACHOWS_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0048894.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    1195..1320
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            1196..1243
                     /gene="pheL"
                     /locus_tag="ACHOWS_003365"
     CDS             1196..1243
                     /gene="pheL"
                     /locus_tag="ACHOWS_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFQ0048895.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            1342..2502
                     /gene="pheA"
                     /locus_tag="ACHOWS_003366"
     CDS             1342..2502
                     /gene="pheA"
                     /locus_tag="ACHOWS_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFQ0048896.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2545..3666)
                     /gene="tyrA"
                     /locus_tag="ACHOWS_003367"
     CDS             complement(2545..3666)
                     /gene="tyrA"
                     /locus_tag="ACHOWS_003367"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFQ0048897.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(3677..4747)
                     /gene="aroF"
                     /locus_tag="ACHOWS_003368"
     CDS             complement(3677..4747)
                     /gene="aroF"
                     /locus_tag="ACHOWS_003368"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFQ0048898.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4957..5322
                     /gene="yfiL"
                     /locus_tag="ACHOWS_003369"
     CDS             4957..5322
                     /gene="yfiL"
                     /locus_tag="ACHOWS_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFQ0048899.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            5472..5990
                     /gene="yfiR"
                     /locus_tag="ACHOWS_003370"
     CDS             5472..5990
                     /gene="yfiR"
                     /locus_tag="ACHOWS_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFQ0048900.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5980..7206
                     /gene="dgcN"
                     /locus_tag="ACHOWS_003371"
     CDS             5980..7206
                     /gene="dgcN"
                     /locus_tag="ACHOWS_003371"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFQ0048901.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            7222..7704
                     /gene="yfiB"
                     /locus_tag="ACHOWS_003372"
     CDS             7222..7704
                     /gene="yfiB"
                     /locus_tag="ACHOWS_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFQ0048902.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     ALSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7781..8128)
                     /gene="rplS"
                     /locus_tag="ACHOWS_003373"
     CDS             complement(7781..8128)
                     /gene="rplS"
                     /locus_tag="ACHOWS_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0048903.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(8170..8937)
                     /gene="trmD"
                     /locus_tag="ACHOWS_003374"
     CDS             complement(8170..8937)
                     /gene="trmD"
                     /locus_tag="ACHOWS_003374"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0048904.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8968..9516)
                     /gene="rimM"
                     /locus_tag="ACHOWS_003375"
     CDS             complement(8968..9516)
                     /gene="rimM"
                     /locus_tag="ACHOWS_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0048905.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9535..9783)
                     /gene="rpsP"
                     /locus_tag="ACHOWS_003376"
     CDS             complement(9535..9783)
                     /gene="rpsP"
                     /locus_tag="ACHOWS_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0048906.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9920..11281)
                     /gene="ffh"
                     /locus_tag="ACHOWS_003377"
     CDS             complement(9920..11281)
                     /gene="ffh"
                     /locus_tag="ACHOWS_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0048907.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            11448..12239
                     /gene="ypjD"
                     /locus_tag="ACHOWS_003378"
     CDS             11448..12239
                     /gene="ypjD"
                     /locus_tag="ACHOWS_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFQ0048908.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            12260..13546
                     /gene="yfjD"
                     /locus_tag="ACHOWS_003379"
     CDS             12260..13546
                     /gene="yfjD"
                     /locus_tag="ACHOWS_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFQ0048909.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13600..14193)
                     /gene="grpE"
                     /locus_tag="ACHOWS_003380"
     CDS             complement(13600..14193)
                     /gene="grpE"
                     /locus_tag="ACHOWS_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0048910.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            14316..15194
                     /gene="nadK"
                     /locus_tag="ACHOWS_003381"
     CDS             14316..15194
                     /gene="nadK"
                     /locus_tag="ACHOWS_003381"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFQ0048911.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            15280..16941
                     /gene="recN"
                     /locus_tag="ACHOWS_003382"
     CDS             15280..16941
                     /gene="recN"
                     /locus_tag="ACHOWS_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0048912.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKHLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELALLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            17090..17431
                     /gene="bamE"
                     /locus_tag="ACHOWS_003383"
     CDS             17090..17431
                     /gene="bamE"
                     /locus_tag="ACHOWS_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFQ0048913.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(17493..17783)
                     /gene="yfjF"
                     /locus_tag="ACHOWS_003384"
     CDS             complement(17493..17783)
                     /gene="yfjF"
                     /locus_tag="ACHOWS_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFQ0048914.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17773..18210)
                     /gene="ratA"
                     /locus_tag="ACHOWS_003385"
     CDS             complement(17773..18210)
                     /gene="ratA"
                     /locus_tag="ACHOWS_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFQ0048915.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            18381..18863
                     /gene="smpB"
                     /locus_tag="ACHOWS_003386"
     CDS             18381..18863
                     /gene="smpB"
                     /locus_tag="ACHOWS_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0048916.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            19078..19440
                     /gene="ssrA"
                     /locus_tag="ACHOWS_003387"
     tmRNA           19078..19440
                     /gene="ssrA"
                     /locus_tag="ACHOWS_003387"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19703..19951
                     /locus_tag="ACHOWS_003388"
     CDS             19703..19951
                     /locus_tag="ACHOWS_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFQ0048917.1"
                     /translation="MRIEICIAKEKICKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIR"
BASE COUNT         5242 a         5249 c         4746 g         4971 t
ORIGIN      
        1 gcgcaaagga aaagtctgcc atctgctgac gtcgatgacg gacgccatgc gcttccccgg
       61 aggagaaatg gcggatctgt acagtcatcg ctgggaaatc gaactgggat acagggagat
      121 aaaacagacg atgcaactga gcaggctgac gctgagaagt aaaaagccgg agcttgtgga
      181 gcaagagctg tggggtgtct tactggctta taatctggtg agatatcaga tgattaaaat
      241 ggcggaacat ctgaaaggtt actggccgaa tcaactgagt ttctcagaat catgcggaat
      301 ggtgatgaga atgctgatga cattgcaggg cgcttcaccg ggacgtatac cggagctgat
      361 gcgcgatctt gcaagtatgg gacaacttgt gaaattaccg acaagaaggg aaagggcctt
      421 cccgagagtg gtaaaggaga ggccctggaa ataccccaca gccccgaaaa agagccagtc
      481 agttgcttaa ctgatcggca ttacagccct tgagctgccg tttttttatt ctgttagttg
      541 tgaaactgaa gcgatttagt cactatcgat ctcatcaaat atggctcgct ttgagatatt
      601 cctcaagtaa aaaaacacct cttcctgcga tttctcacaa aaaagattcg ttgacaaaaa
      661 gtgacaaaat tatgagattt ccatcacaca ttttgacatc aggaacggta tgctgaattc
      721 accaagacgg gaagacaaga ggtaaaattt atgacaatga acattaccag caaacaaatg
      781 gaaattactc cggccatccg ccaacatgtc gcagaccgtc tcgccaaact ggaaaaatgg
      841 caaacacatc tgattaatcc acatatcatt ctgtccaaag agccacaagg gtttgttgct
      901 gacgccacaa tcaatacacc taacggcgtt ctggttgcca gtggtaaaca tgaagatatg
      961 tacaccgcaa ttaacgaatt gatcaacaag ctggaacggc agctcaataa actgcagcac
     1021 aaaggcgaag cacgtcgtgc cgcaacatcg gtgaaagacg ccaacttcgt cgaagaagtt
     1081 gaagaagagt agtcctttat attgagtgta tcgccaacgc gccttcgggc gcgttttttg
     1141 ttgacagcgt gaaaacagta cgggtactgt actaaagtca cttaaggaaa caaacatgaa
     1201 acacacaccg tttttcttcg cattcttttt taccttcccc tgaatgggag gcgtttcgtc
     1261 gtgtgaaaca gaatgcgaag acgaacaata aggcctccca aatcgggggg ccttttttat
     1321 tgataacaaa aaggcaacac tatgacatcg gaaaacccgt tactggcgct gcgagagaaa
     1381 atcagcgcgc tggatgaaaa attattagca ttactggcag agcgacgcga actggccgtc
     1441 gaggtgggaa aagccaaact gctctcgcat cgcccggtac gtgatattga tcgtgaacgc
     1501 gatttgctgg aaagattaat tacgctcggt aaagcgcacc atctggacgc ccattacatt
     1561 actcgcctgt tccagctcat cattgaagat tccgtattaa ctcagcaggc tttgctccag
     1621 caacatctca ataaaattaa tccgcactca gcacgcatcg cttttctcgg ccccaaaggt
     1681 tcttattccc atcttgcggc gcgccagtat gctgcccgtc actttgagca attcattgaa
     1741 agtggctgcg ccaaatttgc cgatattttt aatcaggtgg aaaccggcca ggccgactat
     1801 gccgtcgtac cgattgaaaa taccagctcc ggtgccataa atgacgttta cgatctgctg
     1861 caacatacca gtttgtcgat tgttggcgag atgacgttaa ctatcgacca ttgtttgttg
     1921 gtctccggca ctactgattt atccaccatc aatacggtct acagccatcc gcagccattc
     1981 cagcaatgca gcaaattcct taatcgttat ccgcaatgga agattgaata taccgaaagt
     2041 acgtctgcgg caatggaaaa ggttgcacag gcaaaatcac cgcatgttgc tgcgttggga
     2101 agcgaagctg gcggcacttt gtacggtttg caggtactgg agcgtattga agcgaatcag
     2161 cgacaaaact tcacccgatt tgtggtgttg gcacgtaaag ccattaacgt gtctgaccag
     2221 gttccagcga aaacgacgtt gttaatggcg accgggcaac aagctggtgc gctggttgaa
     2281 gcgttgctgg tactgcgcaa ccacaatctg attatgaccc gtctggagtc acgcccgatt
     2341 cacggtaatc catgggaaga gatgttttat ctggatattc aggccaatct tgaatcagcg
     2401 gaaatgcaaa aagcattgaa agagttaggg gacatcaccc gttcgatgaa ggtattgggc
     2461 tgttacccaa gtgagaacgt agtgcctgtt gatccaacct gatgaaaagg tgccggatga
     2521 tgtgaatcac ccggcactgg attattagtg acggttgtca ttcgcctgac gcaataacac
     2581 gcggctttca ctctgaaaac gctgtgcgta atcgccgaac cagtgctcca ccttgcggaa
     2641 actgtcaata aacgcctgct tatcgccctg ctccagcaac tcaatcgcct cgccgaaacg
     2701 cttatagtaa cgtttgatta acgccagatt acgctctgac gacataataa tgtcggcata
     2761 cagttgcgga tcctgggcaa acagtcgccc aaccatcgcc agctcaaggc ggtaaatcgg
     2821 cgaagaaagc gccagaagtt gctcaagctg aacattttct tctgccaggt gcagcccata
     2881 agcaaaagta gcaaagtggc gcagtgcctg aataaacgcc atattctgat cgtgctcgac
     2941 agcgctaata cgatgcagcc gagcgcccca gacctgaatt tgctccagaa accattggta
     3001 tgcttccggc ttacgtccat cacaccagac cacaacttgt tttgccaggc taccgctgtc
     3061 cgggccgaac atcgggtgta accccagcac cggaccatca tgcgccgcca gcatggcctg
     3121 taatggtcca tttttcaccg atgccagatc gaccagaata caatctttcg gtaaaggcgg
     3181 taatttgcca ataacttgtt cagtaacgtg gatgggcaca ctaacaatca ccattccggc
     3241 atcggcaact atctcagccg ctcgatccca gtcatgttgc tccagaatcc gcacctgata
     3301 acccgatagt gtcagcatct tctcgaacag gcgtcccatc tgaccgccac cgccgacgat
     3361 aaccaccggg cgcagtgacg gacaaagcgt tttaaatcct ttgtcgtttt cactggagta
     3421 agattcacgc atcacccgac gcaaaacatc ctcaatcaga tctggcggta cacccagagc
     3481 ttccgcctct gcacgacgcg aggccaacat agatgcctcg cgctccggaa cataaatagg
     3541 cagtccaaag cggcttttca cctcgcccac ttcagcaacc agttccagac gcttcgctaa
     3601 taaattcagc agcgctttat cgacttcatc aatttgatcg cgtaatgcgg tcaattcagc
     3661 aaccataata aacctcttaa gccacgcgag ccgtcagctg cccgttcaga tcctgatgaa
     3721 tttcacgcag caaggcatcg gtcatttccc agctaatgca ggcatcggtt acggatacac
     3781 cgtatttcat ttcactgcgc ggttgctcgg aagactgatt gccctcgtgg atattacttt
     3841 cgatcatcag accaataatt gagcgattgc catctttgat ttgagcaacc acggattctg
     3901 ccaccgcagg ctgacggcga taatctttat tggaattacc gtggctgcaa tctaccatca
     3961 gagacgggcg cagtcccgcc tgttccatct ctttttcaca ttgcgcaaca tccgcagggc
     4021 tatagttcgg cgctttacca ccgcgcagga tcacatgccc gtccggattc ccctgagttt
     4081 gtagcaacgc cacctgccct gcctggttaa tgccaacaaa acggtgcggc tgggcggcgg
     4141 cacgcatagc gttaatagcc gttgcaaggc tgccgtcggt gccgttttta aaaccaaccg
     4201 gcatggaaag cccggaggcc atttcacggt gtgtttgcga ttccgttgta cgagcaccaa
     4261 ttgctgacca gctaaacaga tcgcccaggt attgcgggct attcggatct aacgcttccg
     4321 tcgccagtgg cagtcccata ttcaccagct caagcagcaa tttacgcgcg atctgcagcc
     4381 cggcttctac atcaaaagag ccatccatat ggggatcgtt aatcaaccct ttccaaccga
     4441 cagtggtacg gggtttttca aaatagacgc gcattaccag atagaggcta tcgctgacct
     4501 ctgcggcaag ggctttaaat cgacgagcat attccagagc agtttccgga tcatgaatgg
     4561 aacaaggacc acatactacc aacagacgag gatcgcgccc ggcgataata tctgaaatgg
     4621 ttttacgcga gtcagcaatc tgggcttcct gttgcaggct taatggaaaa gcggccttca
     4681 gttgttccgg agtcattaaa acttgttcgt cggtaatatg tacgttattc agcgcgtctt
     4741 tttgcatgat ggcgatcctg tttatgctcg tttgcgatag ttgatcctca gcgaggatga
     4801 cgtaacgata acacataaag taaagttttc aatccatatt tcgtacattt ttctttacac
     4861 aagcaattta ttcgcgcttt taacccttgt ttatgtataa ataaatttac accacctttg
     4921 aaaacaatcc gctatgcttt gaaaaaggag aaagaaatga tgaaaaagtt tatcgccccc
     4981 ttgttggctt tactggttag cggatgtcag attgatcctt atactcacgc gccaaccttg
     5041 accagcaccg actggtatga tgttggtatg gaagatgcga tatcgggcaa cgccataaaa
     5101 gatgacgatg catttagcga ttcacaggcg gatcgcggtc tataccttaa aggatatgcc
     5161 gaaggacaaa agaaaacttg ccagaccgat tttacttatg cccgaggact ttccggtaaa
     5221 agctttcctg ccagctgcaa caatgttgaa aatgccagcc aactgcatga agtatggcaa
     5281 aaaggggctg atgaaaatgc cagcacaata cgattaaatt aattaaaccc ccacaaataa
     5341 attaaacatg agattctact cattgtgttt tatttctcac attgatgacg accgcatgaa
     5401 agaatgatat tttaatgagc attctgaaaa aatatcgatg catttcgagc gaagatggtg
     5461 aggatccctg aatgcgtttt tctcaccgac tgttcttact gctgattctc ctgctgacgg
     5521 gagcaccaat actcgctcag gaaccttctg acgttgcgaa aaacgtgcgt atgatggttt
     5581 ccggtattgt cagttacacc cgctggcctg cgctatcagg gccaccaaag ttatgtatct
     5641 tttcatcttc acattttagt aacgccctac aagagaatgg cacgacatct ttgccttatc
     5701 tacctgtcat cattcacact caacaagagg cgatgatttc aggctgcaat ggtttttatt
     5761 ttggcaacga gtcaccgaca ttccagatgg aattaaccga acaatatccg tcaaaggcgt
     5821 tattattaat tgccgaacaa aataccgagt gcattattgg tagcgccttt tgtctaatca
     5881 tccacaataa tgacgtcaga tttgccgtaa acctggatgc cttatcgcgt agcggtgtaa
     5941 aggtcaaccc ggatgtctta atgctcgcac ggaagaaaaa tgatggataa cgataattct
     6001 cttaataagc gccccacgtt taaaagagca ttacgcaaca tcagtatgac cagcatattt
     6061 atcactatga tgctgatctg gttgctgctt tccgtgacct cggtgctgac cctgaaacag
     6121 tacgcgcaaa aaaacctggc actgacagca gcaacaatga cttacagtct ggaagcagct
     6181 gtcgtttttg ccgatggccc tgcagcaact gaaacactgg cagcgctggg ccagcaaggc
     6241 caattttcaa ctgcagaagt gcgtgataag cagcaaaata ttctggcatc ctggcattac
     6301 acccataagg aaccaggcga tactttcagc aatttcataa gccactggct cttccctgcc
     6361 cccatcattc agccgattcg tcacaatggt gaaaccattg gcgaagtacg cttaactgct
     6421 cgcgacagtt caatcagcca ttttatctgg ttttcgctcg ctgtcctgac cggttgtatt
     6481 ctgctggcat caggcatcgc aattaccctc acccgccatt tgcacaatgg cctggtggaa
     6541 gcgctgaaaa atatcaccga tgtcgtacat gatgtgcgtt ctaaccgcaa tttttcccga
     6601 cgagtttcgg aagaacgtat cgctgagttt caccgcttcg ctctcgactt caacagtctg
     6661 ctggatgaaa tggaagagtg gcagcttcgt ttacaggcca aaaatgcgca gcttttacgt
     6721 accgcgctac atgacccatt aaccgggctg gctaaccgcg cagcgtttcg tagcggcatc
     6781 aacacgttga tgaacaattc cgatgcccga aaaacgtcgg cgttactatt tcttgatggc
     6841 gataatttca aatatatcaa tgatacctgg ggtcatgcga cgggcgatag agtcttgatt
     6901 gaaatcgcaa aacggttagc tgaatttggc ggactgcgac ataaagcata ccgcctgggc
     6961 ggcgatgaat tcgcgatggt gctctatgat gtacagtcgg aatctgaagt gcagcagata
     7021 tgctcagcac tgacacaaat ctttaatcgc ccgtttgatc ttcataatgg tcatcagacc
     7081 accatgacat taagcattgg ttacgcgatg accattgagc atgcctctgc ggaaaaatta
     7141 caagagctgg ccgatcacaa tatgtatcag gccaaacacc agcgtgccga aaagctggtg
     7201 agataacaag gatatatcga tatgataaag cacctggtag cacccctgat tttcacctca
     7261 ctaatactga ctggctgcca gtcccctcag ggaaagttta ctcctgagca agtcgccgct
     7321 atgcaatctt atggatttac tgaatccgcc ggcgactggt cgctgggctt atcagatgcc
     7381 attctgttcg caaaaaatga ctacaaattg ctcccggaaa gccagcaaca gatccaaacc
     7441 atggcagcca aattggcctc gacagggcta acgcatgccc gtatggatgg acacaccgac
     7501 aactatggtg aagacagtta caacgaagcc ttatcattga aacgggcgaa tgtcgtggcc
     7561 gatgcatggg ctatgggtgg acaaattccg cgcagcaatc tcaccacaca gggtttagga
     7621 aaaaaatatc ccatagccag taacaagacc gcccagggcc gcgccgagaa ccgccgcgtc
     7681 gcagtggtga ttactacccc ttaaaacacg agcaaacaga taaaaaaagg gccagccaat
     7741 tggccagccc ttcttaacag gatgtcgctt aagcgaaatc ttagttaaga cgctctttga
     7801 tacgagcagc cttaccagta cgctcacgca ggtagtacag tttagcttta cgaacagcac
     7861 cacgacgttt gacagaaatg ctgtcaacta ccggagagtg agtctggaag acacgctcaa
     7921 cgccttcgcc gttggaaatt ttacgaacag tgaatgcaga gtgcagaccg cggttacgaa
     7981 tagcgataac cacgccctcg aatgcctgca gacgtttttt ggaaccttca acaacccata
     8041 ctttcacttc cacggtatca cccggacgga aggaaggtac gtcctgcttc atctgctctt
     8101 gttcaagttg cttaataatg ttgctcataa tttaatctct tatcctgggt aaactgatat
     8161 ctcgggggct tacgccatcc catcatgttt atgttgctgt tgtgcgtgtt ccgttttgaa
     8221 ctccgccagc aaccttgctt gctcttcagt cagagccagg ttttccagaa gttcaggtct
     8281 tctaagccag gtacggccca gcgactgttt caaacgccag cgacgtatct cggcatggtt
     8341 gcccgacagt aacactggcg gaacttccat cccttctaac acctcaggcc gcgtatagtg
     8401 cgggcaatcc agcaatcctt cagcaaagga atcttccgtt gccgaggctt catgtcccag
     8461 tactcccgga ataaaccggg aaacggagtc aatcagcgtc attgctggta actcaccacc
     8521 actgagaacg taatcgccga ttgaccattc ttcgtcaatt tcggtttgga tcacgcgctc
     8581 atctatacct tcgtagcgac cgcacaccag aatcaatttt tgattcgttg ccagttcgct
     8641 gacgcccgct tgatcaagct tgcgtccctg tggtgacaga taaatcacct ttgcgccttc
     8701 acccgccgcg gcttttgctg catgaatggc gtcccgcaag ggttgcacca tcattaacat
     8761 ccccggtccg ccgccgtaag gacgatcgtc cacggtacgg tgccggtcat gcgtgaagtc
     8821 gcgaggactc cagctctgga tgctcagcag gccattttta actgcccggc cagttacccc
     8881 gtaatcggta attgcgcgga acatttcagg aaacaggcta attatgccaa tccacatagc
     8941 gccgtctttt accgtttatc cggtggttta aaaaccagga tcccaatcta cttcgattga
     9001 acgagtagtg agatcgactt tcttgataac ctgcccatcg aggaacggta cgagacgttc
     9061 cttgatacca aacgcatctt tcaggtttgc cttaatgacg agaacgtcat tagatccggt
     9121 ttccatcata tcgacgactt taccgagatc gtagccttca gtggttacta cctggcagcc
     9181 catcaagtct ttccagtagt agtcaccctc ttcaagctga ggcaactgcg atgaatccac
     9241 gacaatttca caattcgtca gcaggttcgc cgcatcacga tcgtcaacgc ctttcagctt
     9301 gatgatcatg tcctgattgt ggtgcttcca gctttccagc tggacttgct gccactgacc
     9361 cgccttctgg ataaaccagg gctgatagtc aaaaatgctt tcggcgtctt cggtggaaga
     9421 aaacactctg agccacccac gaataccgta agacgaaccc atttttccca aaacgatggg
     9481 atcaacaggt gcttgcgcgg tgagttgttt gctcatcatg accaccgtga cagattaagc
     9541 tgctttgttt acttctttga tcagcgcagc aacgcgatca gaaatagttg cgccctggcc
     9601 aacccagtga gcgatgcgat ccagatccag gcgagtgcct tcttcttttt cgctagcgat
     9661 tgggttgaag aaaccaacgc gctcgatgaa gcgaccgttg cgtgcattac ggctgtcagc
     9721 gacaacaacc tggtagaacg gacgcttttt agcgccgtga cgtgctaaac gaatagttac
     9781 cataacatcc tcttgtgtga ataaaacaac cggaccccat cgaggaacgg agtccggtgt
     9841 catattaaaa gcccgaaaat tttactgatt tctggagaaa atgcaatcag cagttaataa
     9901 ctctgtcgca taaggtcatt tagcgaccag gaaatcccgg cggcatcata cccttcatgc
     9961 ttctcatcat cttcgccatt ccgcccttct tcattttctt catcatgcgc tgcatgtcgt
    10021 cgaactgttt cagaagacgg ttaacgtcct gcacctgcat accgcaaccg gcagcgatac
    10081 ggcgtttacg cgaacctttg atgatttctg gcttagcgcg ctctttcatc gtcatcgagt
    10141 tgatgatggc ttccatacgc accagcactt tatcgtccat ctgcgacttg acgttatccg
    10201 ggatctgccc catgcccggc agtttgccca tcaggctggc catgccgccc atatttttca
    10261 tctggcgcag ttgctcaagg aagtcattga gatcgaagcc gtcgcctttt ttcagcttgc
    10321 tggctaattt ctcagcttgc gcgcggtcaa ctttgctttc gatatcttcg atcagcgaca
    10381 gtacgtcgcc catgccgaga atacgggaag cgatgcggtc cggatggaac ggctccagcg
    10441 cctcggtttt ctcgccaaca ccgaggaact tgatcggttt gcccgtaatg tgacgaatag
    10501 agagcgccgc accgccacga gcatcgccgt cgactttagt cagtaccaca ccggtcagcg
    10561 gtaacgcttc attgaatgct tttgccgtat tggccgcatc ctgaccagtc atggcatcaa
    10621 ccacaaacag ggtttcaacc ggattaatcg acgcatggac ttgtttgatc tcgtccatca
    10681 tcgcttcgtc aacgtgcaga cgaccggctg tatccaccag cagcacgtcg tagaatttca
    10741 gtttggcttc tttcagcgcc gcgttaacga tatctaccgg cttctgacca acatcagaag
    10801 ggaagaaatc aacgcccacc tgctctgcca gcgtctcaag ctgtttgatt gccgccggac
    10861 gataaacgtc ggcagaaacc accagcactt tcttcttgtg cttctcgcgc aggaacttac
    10921 cgagtttacc aacgctggtt gttttaccgg caccttgcag gcccgccatc agcacgaccg
    10981 caggcggttg cgcagccagg ttcagggtct ggttctcttc gcccatcgcc gcaaccagtt
    11041 cgttacggac aattttgacg aactcctgcc ccggcgtcag gctcttatta acttcatgac
    11101 caaccgcttt ctcttttacg cgattgataa actcacgcac taccggcaga gcgacgtccg
    11161 cctccagcag cgccatgcgc acttcgcgca gcgtatcttt tacgttgtct tcagtgaggc
    11221 gtccacggcc actgatattg cgcagcgtgc gcgacaaacg atcggttaaa ttatcaaaca
    11281 ttgtctctcg cctggggtgg aaacggttgg ccgcaatcgc gacacatcat cagtattttg
    11341 ccgcagtata acatgaaggc gtctttgttg ttatgcaacg gttggagcag cgttcacctg
    11401 acgctatact gcttctcttt cttattgctc aaactgtcga cattactatg cccgtttttg
    11461 ctctgctcgc gcttgtcgcc tactccgtca gtcttgcgct gattgttccc ggtctgctgc
    11521 aaaaaaacgg cggctggcgg cgcatggcta ttatttctgc ggtcattgcg ctggtctgcc
    11581 acgcaatcgc tctggaagcc cgcatcctgc ccgacggtga tagcggacaa aacctcagcc
    11641 tgctgaacgt tggttcattg gtcagtttga tgatctgtac ggtaatgacc attgtggctt
    11701 ctcgcaatcg tggctggttg ctgctaccca ttgtctatgc ctttgcgctt atcaacctgg
    11761 cgctggcgac cttcatgccc aatgaataca tcacccatct ggaagcaacg cctgggatgc
    11821 tggtgcacat tggcttatcg ctcttttcct atgccacgct gattatcgcc gccctgtacg
    11881 cgctgcaact ggcgtggatt gattaccaac tgaagaacaa gaagctggcg tttaaccagg
    11941 aaatgccgcc attgatgagt atcgagcgta aaatgttcca catcacgcag attggcgtgg
    12001 tgctgctaac gctcacgctt tgcactggcc tgttctacat gcacaactta tttagcatgg
    12061 aaaatatcga caaggccgtg ctctctatcg tggcgtggtt tgtctatatt gtgctgctgt
    12121 ggggacatta tcatgaagga tggcgtggac gccgcgtcgt ctggtttaac gttgcgggtg
    12181 cagtcattct gacactggcc tacttcggca gccgaattgt ccagcagtta atcagctaaa
    12241 cccagaaaag gagtttcccc tggaacacat ttctactact acgttgatca ttattctgat
    12301 catcatggtg gtcatttcag cctatttttc cgggtccgaa accggaatga tgaccctcaa
    12361 ccgctatcgt ctgcgacata tggcgaaaca gggtaatcgc tcggccaaac gcgtcgaaaa
    12421 attgctgcgt aagccagacc gcctgattag cctggtgtta atcggcaata acctggtcaa
    12481 tattcttgcc tccgcgctag gcactattgt tgggatgcgt ttgtacggcg atgcgggcgt
    12541 ggcaattgcg actggtgtgc tgacttttgt cgtgctggtt tttgctgagg tattgccgaa
    12601 aaccattgcc gcgctgtacc cggaaaaagt cgcttatccg agtagttttc tgctggctcc
    12661 gctgcaaatt ttgatgatgc cgctggtctg gttgctgaat gctatcaccc gtatgctgat
    12721 gcgcatgatg ggtatcaaaa ccgatatcgt ggttagcggc tctttgagca aagaagagtt
    12781 gcgcactatc gtgcacgaat cgcgctcaca aatttcccgt cgcaatcagg atatgctgct
    12841 gtcggtgctc gatctggaaa aaatgaccgt tgatgacatc atggtgccgc gcagtgaaat
    12901 tatcggtatt gatatcaacg atgactggaa atcgattctg cgccaactct cccactcacc
    12961 tcacgggcgc atcgtgctct accgtgattc gctggacgac gccatcagta tgctgcgtgt
    13021 gcgtgaagcc tggcgactca tgtcggagaa aaaagagttc accaaagaaa ccatgctacg
    13081 cgccgcggac gagatctatt ttgtcccgga aggtacgccg ctcagcacgc agttggtgaa
    13141 gtttcagcgc aacaaaaaga aagtcggcct ggtcgtcaac gagtatggag acattcaggg
    13201 gctggtgacg gttgaagata ttctggaaga gattgtcggc gatttcacta cctcgatgtc
    13261 gccaacactt gccgaagagg tcacgccgca aaacgacggt tcggtgatta tcgatggcac
    13321 cgccaacgtg cgagaaatca acaaagcctt taactggcat ctacctgaag acgatgcccg
    13381 cacggttaat ggcgtcattc tggaagcgct ggaagagatc cctgtcgctg gcacccgcgt
    13441 gcgtattggc gagtacgata tcgatattct cgacgtacag gataatatga ttaagcaggt
    13501 aaaagttttt cctgtgaaac cgctgcgcga gagtgtggcg gagtaaaaaa acggcccggc
    13561 attcgcatgc tgggccgtga tttattacga aagcagaaat taagcttttg ccttcgctac
    13621 agtcaccatc gccgcacgaa tcgtacgacc gttcagcgta taacctttct gcataatgcc
    13681 cagtacgtta cctggcgcaa cgtcatcaga ttccaccatt gcgatggcct gatgcacatt
    13741 tgggtccagt gggacgttgg tttcggcgat cacttcaacg ccaaacttac gcacaacatc
    13801 cagcatcgac ttcagcgtca gctcaatacc ttcaaccatc gcggacatat ccgggttagc
    13861 tttatcagcc acttccagcg cacgatccag gctatcaatc accggcagca attcgttgat
    13921 gaatttctcc agcgcgaatt tgtgagcttt ttcaatatcc agttcagtac gacgacgcag
    13981 gttttccatt tcggctttta cgcgcaaaat gccgtcacgt tcacgggtct gggcttcagc
    14041 cagctgagct tcgagattcg caattttttc atcgcgcgga tccacctgct cagcagaagc
    14101 ttctggctca actgcctcaa tctcttcgtg ctgatccatg ataatttctt ccggggcttg
    14161 cccctcaggc gttttctgtt ctttactact catgaatttc tccgcgtttt tttcgcattc
    14221 atctcgctaa cttcgcttat tatggggatc agtttcaggg tttcaaggga agcactcaca
    14281 ttgtcatcaa tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc aagtgtattg
    14341 gcattgtggg acacccacgg caccccactg cactgacaac acatgaaatg ctctaccgct
    14401 ggctgtgcac aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac gaactgcaac
    14461 tgaagaatgt gaaaactggc acgctcgcgg agattgggca actggcagat ctcgcggtag
    14521 tcgttggtgg cgacggtaat atgctgggcg cggcgcgcac actcgcccgt tacgatatta
    14581 aagttattgg aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac cccgataacg
    14641 cccagcaaca attagccgat gtgctggaag gccactacat cagcgagaaa cgttttttgc
    14701 tggaagcgca agtttgtcag caagattgcc agaaacgcat cagcaccgcg attaacgaag
    14761 tggtacttca tcctggcaaa gtggcgcata tgattgagtt cgaagtgtat atcgacgaga
    14821 tctttgcgtt ttcgcagcgt tccgatggac tgattatttc gacgccaaca ggctccaccg
    14881 catattccct ctctgcgggc ggtcctattc tgaccccctc tctggatgcg attaccctgg
    14941 tgcccatgtt cccgcatacg ttgtcagcac gaccactggt gataaacagc agcagcacga
    15001 tccgtctgcg tttttcgcat cgccgtaacg acctggaaat cagttgcgac agccagatag
    15061 cactgccgat tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac catctgaatc
    15121 tgattcatcc gaaagattac agttatttca atacattaag caccaagctc ggctggtcaa
    15181 aaaaattatt ctaattttac gccagcctct ttactgtata taaaaccagt ttatactgta
    15241 cacaataaca gtaatggttt ttcatacagg aaaacaacta tgttggcaca actgaccatc
    15301 agcaactttg ctatcgttcg tgagcttgag attgattttc atagcggcat gaccgtaata
    15361 actggcgaga ccggcgcggg taaatctatt gcaatagatg ccctcggcct ttgtctcggt
    15421 ggtcgcgctg aagccgacat ggtgcgtacc ggcgctgctc gtgccgacct gtgcgcccgt
    15481 ttttctctga aagatacgcc agcggccctg cgctggctgg aagaaaacca gcttgaagac
    15541 ggtcatgaat gtttgcttcg tcgcgtaatc agtagcgatg gtcgctcacg aggttttatc
    15601 aacggtacag ctgttcctct gtcacaactt cgcgaactgg gccagttgct gattcagatc
    15661 catggtcagc acgcccatca gttactcacc aaacccgagc accaaaaatt cctgcttgat
    15721 ggctatgcca atgaaacctc tctgctccag gaaatgaccg cacgttatca gttgtggcat
    15781 caaagctgcc gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc cgcccgtgcg
    15841 gaactgctgc aataccaatt aaaagaactt aacgaattta atccgcagcc cggagagttt
    15901 gagcaaatcg acgaagagta caaacatctg gcgaacagcg gtcaattgct gactaccagc
    15961 cagaatgcat tggcagtaat ggccgacggt gaagacgcaa acctgcaaag tcagctttac
    16021 acggctaaac aactggtgag cgaattgatt ggcatggaca gcaaactgtc cggcgtactt
    16081 gatatgctgg aagaagctac catccagatt gctgaagcca gcgatgaact gcgccactac
    16141 tgcgatcgtc tggatctcga tcccaaccga ctgtttgaac ttgaacagcg catctcaaaa
    16201 cagatttcgc tggcacgtaa acatcacgtc agccctgagg cattgccaca gtattaccag
    16261 tcgctactgg aagaacagca gcaactggac gatcaggccg actcacaaga aacgctcgcg
    16321 ctggcggtaa cgaaacatca tcagcaggca ctggaaaccg cgcgcgcatt acaccagcaa
    16381 cgccagcagt atgcagaaga acttgcactg ctgatcaccg acagtatgca tgcgctctca
    16441 atgccgcatg ggcagtttac gatcgatgtt aaatttgacg agcatcacct gggcgctgac
    16501 ggtgccgatc gtattgagtt tcgggtaacc accaacccag gtcagccaat gcagcctatt
    16561 gccaaagtcg catccggtgg tgaattgtca cgcatcgcat tggcaattca ggtcatcacg
    16621 gcgcgtaaaa tggaaacccc ggcactgatt tttgatgaag tggatgtagg gattagcggt
    16681 ccaacagcgg cagttgtcgg caaactgctg cgtcaacttg gcgaatcaac tcaggtgatg
    16741 tgtgttaccc acctgccaca agtcgcggga tgtggtcatc aacactattt tgtcagcaaa
    16801 gaaaccgatg gtgcgatgac agaaacacac atgcaatccc tggataaaaa agcgcggtta
    16861 caagagctgg cgcgcctgct tgggggcagt gaagtcacac gtaacacact ggcgaatgcg
    16921 aaagaactgc ttgcggcgta aacttttttc ctgcttcacg gtcagagtaa acagcaaaac
    16981 gccgtaagac cggaaagcaa aaggttttaa agtgatgaaa ggtctattat catcggcata
    17041 ttacagatga gccacgtact gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa
    17101 aacgctgact gctgcagcag cagtactatt gatgttgacc gcaggctgtt ccactctgga
    17161 gcgagtggtt taccgtcctg acatcaacca ggggaattat ctgaccgcta acgacgtatc
    17221 caaaatacgc gttggcatga cgcaacaaca agttgcgtac gcattgggta caccgctgat
    17281 gtccgatcca tttggtacga atacctggtt ctatgtcttc cgccagcaac caggtcatga
    17341 aggtgtaacg cagcaaacgc tgacgctgac ctttaatagt agcggggtgt tgaccaatat
    17401 tgataataaa cctgcgctga gtggtaacta atcataaagt tgctctcaaa gacgttaaaa
    17461 aaggtgctca atgagcacct tttttctgtc tgttatttat tcgctgattt ttctgctcgt
    17521 tgcctgcgca gctctttcgg atcggcaatg agagggcgat aaatctccac ccgatcgcca
    17581 tcatgcacac tatcgctcag ttttgccgga cggctataaa tgccgacttt gtttttagtt
    17641 aaatcgatat cggtacgcaa ttccagcaag ccactggcgc gaatagcttc ttcaaccgtc
    17701 gcaccctcct gcagcgtcac acgctgcagg tactgcttct caggtagcgc ataagccacc
    17761 tcaacggcaa ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa aagcctggac
    17821 catattagcc gccagttctt taaacacgcg gccaaaggcg agttcaatca acttattggt
    17881 aaactcaaaa tcgagatgaa actcgatacg gcacgcctcc tgactcagcg gcgtaaactt
    17941 ccatccaccg atcaatttct tgaacggccc atccaccaga ttcataagaa tactttggtt
    18001 actggtcaac tggttgcggg tagtaaacgt tttgctgatc ccagccttag agacatctac
    18061 cgccgcagtc atctgcccag gagtggactc cagaatccga cttccggtac aacccggcaa
    18121 aaactgagga taagactgaa cgtcattcac taactgatac atttgctccg cgctgtaggg
    18181 taccagtgcg gtccggctaa tctgaggcat aacaatttcc atcaacaaaa atccaacaaa
    18241 taatatcatt tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa catcgggttc
    18301 atgctaagat agagccttgt cccccgcagg attgatatgg ggtgttttcg atttcagatt
    18361 accgatgatt cacgacgctt atgacgaaga aaaaagcaca taaacctggt tcagcgacca
    18421 tcgcgcttaa caagcgcgcc cggcacgaat actttatcga agaagagttc gaggcgggac
    18481 ttgccctgca aggctgggag gttaaatccc tgcgtgcagg aaaagccaat atcagcgaca
    18541 gctacgtcct tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc acgccaatgg
    18601 ctgtggcctc cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc
    18661 agcgcgaact ggactcattg tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc
    18721 tctccctgta ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaga
    18781 aacagcatga taaacgttca gatatcaaag aacgcgaatg gcaggtggat aaagcgcgta
    18841 tcatgaaaaa cgcccaccgt taaacctgca ctgcaattat tgatccgctc ctcaccgcgc
    18901 ctccctctcc ggcggcgcga atgcacatct tattggctat cacatccgac ccaaatgttg
    18961 ccatcccatt gcttaatcga ataaaaatca ggctacatgg gtgctaaatc tttaacgata
    19021 acgccattga ggctggtcat ggcactcatg aatctggtat acttaccgtt acacattggg
    19081 gctgattctg gattcgacgg gatttgcgaa acccaaggtg catgccgagg ggcggttggc
    19141 ctcgtaaaaa gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat
    19201 aacctgctta gagccctctc tccctagcct ccgctcttag gacggggatc aagagaggtc
    19261 aaacccaaaa gagatcgcgt ggaagccctg cctggggttg aagcgttaaa acttaatcag
    19321 gctagtttgt tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag actgactaag
    19381 catgtagtac cgaggacgta ggaatttcgg acgcgggttc aactcccgcc agctccacca
    19441 aatataacag gactacagct agactttaac agtaaaaaca agaaattaca gacattgaat
    19501 agactgtagg tagacctgaa aaagacaaaa atatacacgc aatatgcacg tgcattttaa
    19561 aaaaccccag atttctccgt ctgggttttt tctatttgta aaaaagtgaa acagattaca
    19621 ttacaataat gacgctcttg acactgttta tgcatacagt aaaaaattat tcctgtacat
    19681 aaccacagtg acaggaggtg ttatgcgtat tgagatctgt atcgctaaag aaaaaatctg
    19741 caaaatgcca aaaggctctg ttcctgccct gcaacaggaa atgctacgac gtgtcggtaa
    19801 gcgttatgat gatgtagatg tgattgtgaa atctgccagt aatgatggac tcacaatttt
    19861 aagagccgtg gataaagaat ccgcgcaaga atttgtacag gaaacgctgc aggaagtctg
    19921 ggaaacagcg gatgactggt ttatccggtg acatcagcac ggggagctgg cagctcccct
    19981 cttttattgt gttatcagtg cagcaagtac acagagtgtt accacaccac ccagccatac
    20041 aaacagcatg aagcacatcg cacccgctgt gtatttccgc tttccgcatc tgattttttc
    20101 ctgctccatt ctcactgcca ccgttcaact ctcattaccc gacttcacga atatgcattc
    20161 cggctacgca cgtccaacca ggtgcttcac actcctgtac ttaagacc
//
LOCUS       DAZKJR010000093        20112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKJR010000093 DAZKJR010000000
VERSION     DAZKJR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.093"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..798
                     /locus_tag="ACHOWS_003389"
     CDS             <1..798
                     /locus_tag="ACHOWS_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFQ0048918.1"
                     /translation="PPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTSV
                     VPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQF
                     NGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQ
                     GPASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLLV
                     RSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            802..2133
                     /gene="traO"
                     /locus_tag="ACHOWS_003390"
     CDS             802..2133
                     /gene="traO"
                     /locus_tag="ACHOWS_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFQ0048919.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     NNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            2130..2843
                     /gene="traP"
                     /locus_tag="ACHOWS_003391"
     CDS             2130..2843
                     /gene="traP"
                     /locus_tag="ACHOWS_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFQ0048920.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCLLIIL
                     AAVWYKFLRAPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            2840..3370
                     /gene="traQ"
                     /locus_tag="ACHOWS_003392"
     CDS             2840..3370
                     /gene="traQ"
                     /locus_tag="ACHOWS_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFQ0048921.1"
                     /translation="MMRIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            3417..3815
                     /locus_tag="ACHOWS_003393"
     CDS             3417..3815
                     /locus_tag="ACHOWS_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFQ0048922.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIAQVIGLVLFIAGLLGLKQIGKKPGAGLACIL
                     SIVVGACLAVVPEMMSSGQKTLGLSPVSIS"
     gene            3872..4123
                     /locus_tag="ACHOWS_003394"
     CDS             3872..4123
                     /locus_tag="ACHOWS_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFQ0048923.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            4038..4856
                     /locus_tag="ACHOWS_003395"
     CDS             4038..4856
                     /locus_tag="ACHOWS_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFQ0048924.1"
                     /translation="MNKTPPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILMPDSFPDKKYVM"
     gene            4911..5270
                     /locus_tag="ACHOWS_003396"
     CDS             4911..5270
                     /locus_tag="ACHOWS_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048925.1"
                     /translation="MDTLTAGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            5270..5650
                     /locus_tag="ACHOWS_003397"
     CDS             5270..5650
                     /locus_tag="ACHOWS_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048926.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     RNERRKCIVVHINKITYPPVNSLR"
     gene            5826..8870
                     /locus_tag="ACHOWS_003398"
     CDS             5826..8870
                     /locus_tag="ACHOWS_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0048927.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEVMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            8870..9487
                     /locus_tag="ACHOWS_003399"
     CDS             8870..9487
                     /locus_tag="ACHOWS_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFQ0048928.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWQDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            9448..10653
                     /gene="traW"
                     /locus_tag="ACHOWS_003400"
     CDS             9448..10653
                     /gene="traW"
                     /locus_tag="ACHOWS_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFQ0048929.1"
                     /translation="MQRQPATTLLALLIGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPIREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            10650..11219
                     /gene="traX"
                     /locus_tag="ACHOWS_003401"
     CDS             10650..11219
                     /gene="traX"
                     /locus_tag="ACHOWS_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFQ0048930.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            11292..13451
                     /locus_tag="ACHOWS_003402"
     CDS             11292..13451
                     /locus_tag="ACHOWS_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFQ0048931.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     YYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTTASSTEYRTAEAAEPKSIIMKVWSGVNFADKIATSLSGTGTTSD
                     QLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAKAF
                     LEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTNQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            13533..14180
                     /gene="excA"
                     /locus_tag="ACHOWS_003403"
     CDS             13533..14180
                     /gene="excA"
                     /locus_tag="ACHOWS_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFQ0048932.1"
                     /translation="MNTTKSRYPSWWAYYQLARNTYFIFGIPCLLFYAFFGTLIVHDS
                     SLSQTRDFIFAYGCWALIILPLLWLYLKTRAKKKKVQQVVQQIKESSPFAPTNDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(14494..14646)
                     /locus_tag="ACHOWS_003404"
     CDS             complement(14494..14646)
                     /locus_tag="ACHOWS_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFQ0048933.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            14979..15221
                     /locus_tag="ACHOWS_003405"
     CDS             14979..15221
                     /locus_tag="ACHOWS_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048934.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(15314..16849)
                     /locus_tag="ACHOWS_003406"
     CDS             complement(15314..16849)
                     /locus_tag="ACHOWS_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFQ0048935.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNALGNVLILFLMMLLGRRNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGHKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(17049..17504)
                     /locus_tag="ACHOWS_003407"
     CDS             complement(17049..17504)
                     /locus_tag="ACHOWS_003407"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFQ0048936.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVIYILITRLNLTTTYYSL
                     YSSLDENSQVTEYFIISIATFLFLAVLCLFFIYSFFKRKRVVGKLLLCIYILYISHYA
                     ITFVYCLYVVGAECMATGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(17592..18215)
                     /locus_tag="ACHOWS_003408"
     CDS             complement(17592..18215)
                     /locus_tag="ACHOWS_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFQ0048937.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRESGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            18364..18849
                     /locus_tag="ACHOWS_003409"
     CDS             18364..18849
                     /locus_tag="ACHOWS_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFQ0048938.1"
                     /translation="MIKYIFALTLLLSGSVCADQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVVNTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNSHNNY
                     K"
     gene            19493..19857
                     /locus_tag="ACHOWS_003410"
                     /pseudo
     CDS             19493..19857
                     /locus_tag="ACHOWS_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19867..20058
                     /locus_tag="ACHOWS_003411"
     CDS             19867..20058
                     /locus_tag="ACHOWS_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048939.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPGGE"
BASE COUNT         5136 a         5580 c         4930 g         4466 t
ORIGIN      
        1 ccgccacctg cctctcccct tgttcaggaa gccgcatcac tggattcccc cctgaatgca
       61 ggtgaaattc gggaactgcg gggacgaatg gccgataacg aacgggccat cagcgcacct
      121 gtcaccagtg tggtgccccg tatcagctcc ctcacggtaa atctgtcacc cggggccagt
      181 ctgccgcttg tccgaaccgc actgcataac gtcagcgtgg taacgttcac ggacatcaac
      241 ggctccccgt ggcctcagtc ggatccgcct tataacgcgg cacccggtca ctttgatgtg
      301 cagttcaacg gcaatatggt ctccgtcact cccctgcgcg cctgggctgc agggaatgta
      361 acggtctacc tcaaaggact gtctgtaccg gtcattctga atatcaccag tggtgaaacc
      421 gacaccaata ccctgagtca ggagatcgac agccgtctgg atctgcgcat tccccgtcag
      481 ggacctgcca gtcctgtggt agccgtacca tcagagaaga ttgccctgca tgatgccacc
      541 ctgcaggcct ttctggacgg tgttcctccc gatgatccca ccgtgcgtcg gctgaaattc
      601 tccggaaatg ttccgggcac caccatctgg cagcacggcg atgatctgct cgtgcgatcg
      661 cgggccatgc tgcgggatga atttgaacag acgctggcat ctgctgacgg tacgcgtctg
      721 tggaaactgc cggtgactcc cttactcacc ttctccgtga atggtcagtc cattcacgtc
      781 acccctgaac tggagtaaat catgagcgca gaacaggaca cagccgcaaa gtccggcaaa
      841 aaactgtcag ccctgctggt tctcgggggc gtgattatca tcgggggcgg atacctgctg
      901 ctcacctggc tttcccccga aaacagcgca tcgctctcgt cagtcaatat caacagcacg
      961 acaacaggca gcgggcggac cgtggtggag tcacagcact accgggattt actgcgtgct
     1021 gacaatgagc gcggggccgc agaagcgtta cgtaataacc agacgttcat cgccagtctg
     1081 cccaggggac tggatactga tcacggaaat aatcaaagaa gggatactca gcctgctgac
     1141 caggccgagg gttctggtca accatcaacg ccccctcagg cagatcaaac agcccgggaa
     1201 aaacagcggg aagcgttaca aaaactgctt gcccgcatca acacacagca tcctgacgcc
     1261 cgccccccgg tagtggcggc tgcaatgtgg ggtaccactg gtgctcccgg gataagctca
     1321 cacgcctcac cacccggtac cggcacatca ccacaaccac gtctgcagga ggccagtctg
     1381 gcaaccccgg cagctcgtac agggatacag ctgataccgg cactgacccg cgtacctgcc
     1441 ttcattgata cggaagtgga ctctgacaat gctgtctccc gcgtggtggc ctccgtacct
     1501 gccggcccat gggccggggc cgtcctctac agccaggacg ccagactcgc cggcaaggga
     1561 atggatatcc actttgaccg gatgatgtgg cggggcatgt ttctgaaagt caatgcatat
     1621 gccctgaatg aacataccgg catgtcatcc gtggccagcg acgtgaacca ccgctggttt
     1681 aagcatatcg ttctgccgtc tgtgctcggt ggggtgggca gtgtcggaag tctttacaaa
     1741 gacgccaaca cacaggtcat tcagggaaac tacggcagca ttaccggcag ggtgggaatg
     1801 cctgatggcg aggcgctggc tggcgtcatt gcaggcggca tggccgaacg gggcagtcag
     1861 ttgctgaccc gccaggcgga gagcgaacct taccggcagg tgacagtgtc acgcggagag
     1921 gttgtctcca ttctgtttgt tgagccggtc atgagcaacg atatcagtcc cccctcctcc
     1981 ggcactgccc cttctgtgcc accgccctcc ggacgcccgc tcacccagga agaagcagag
     2041 cgtcaggcgt ctgacaggat ccaggcagct gtcatgcgta aacagaacga aatgcggcaa
     2101 cgttacggaa tacaacagga aagtcatcca tgaaaaacga acatgaagat cccggcagtt
     2161 ttggcgaaac aacgacaccg gcatccggca aatccttcct gaaacgccag tatatgggat
     2221 acagcctgcc agtctgggcc ggctgtctcc tcatcatcct ggccgcagtc tggtacaagt
     2281 ttctgcgggc accctcagag ccccctgcag atgcaggctt cgctgtcaca ccgcccgcag
     2341 ccgaaagctg ggcagagccc atgcccatga cggagaccgg gagcggaaac accatgtccg
     2401 cgcctcctgt caccccacag gcagacatgc agcagcttgc ccgtgacctg aaagcagagc
     2461 tggacaggcg ggatgaggaa atccgacgta ccctgaccat gctgaaagag agcataacaa
     2521 cccttggcga caccatacgg aaagatgagg cctgggcaca ggaaacccgt cagttgcttg
     2581 atgaaacccg taagcaggtg catgccctgc agatgcaggg accactgcaa atcccgaccg
     2641 ggaataacgg ccagtcaacg aaaaccccgc cttcccgcct gcagggcatg caaatcatgt
     2701 cggtggaaaa aggaatggcc tggatccgct ggcagggtag cgcctgggca gtacgggaag
     2761 gtgacaggct ggggaatatc accatcaccc ggattgaacc gtccagccgc tcagtcatca
     2821 cctctgccgg tacattacga tgatgagaat tgatgccctg acagccatag agaactttgc
     2881 cagcggtata ttcagtgcag gcatggagtt tctcttcacc tggggagagc tgctggggat
     2941 tatcggcctg attggtcatc tgatgcgtgc ccgggctgaa gggcgtcaca gtatgggacc
     3001 gggaaaattt atcgccggta tcgtcatctg cggcatgctg gtcgccctgc catccttcat
     3061 caatgccggg gggacccaga tgggattcag ggcagattcc ttcggcccta tcgcttatgt
     3121 acaaccaacc acctttggtg ccgctgccgg tgccgccaat gccatgctct cactggtaaa
     3181 actggccgga gtggggtttg ccatgaacgg catttccatc tggcgtaaat ccctgctgga
     3241 cgggcatacc gccctgagca gcagtgagtc catcagcaaa ggaaacgtca agtttgttgc
     3301 cggcgttctg ctggttttta tcgatcgcgt catcgatgcc tctctggcga gtcttggtct
     3361 cgccttctga tccacattca ttttcagtaa ttcagtctgt tagttaagga gactatatgt
     3421 cacgattccg tgccatggcg gcgcgtctgt gcgctgcatt tatttatgtt cagttatgcc
     3481 tggtggcctg ccgtgaccgg ctgatacgcc tgttacttct gcttcccgtg atactgctgc
     3541 cccacacagc gatggcaaac accctattcg atatgatcaa aaccgttggc gatggtgcaa
     3601 aaggctccag cagttcattt gtggccatcg ctcaggttat tggcctggtg ctctttattg
     3661 ccggcctgct tggcctcaaa caaatcggta aaaaacccgg agcagggctg gcctgcatac
     3721 tgtccattgt ggtcggtgcc tgtcttgccg tcgtaccgga aatgatgagc agtggacaga
     3781 aaacgctcgg tctttctccg gtctccatca gttaatccac aacggcaccg gtttcccggt
     3841 gccgtcttca cacaatgaag aggaccagac catgtcctgt gagctgcccc ctttcaccct
     3901 gctcagaaca gagcacggca acgtgattgc ccgcgacaac cgggataatc aggagtttgt
     3961 gtttgcagaa tgttattcca ccgccatcgc agcactgatt ctggctgccg tcaccctgta
     4021 tacagagaga aaaacgtatg aacaaaacac cgccactcat cgccccggcc cgggaacgct
     4081 ggcttttccc gctcccgcaa ccggacacgg tattcagcgg tgaatccctg ctgccaccac
     4141 ccgtgctctc aacacccgga cgctgtccct gctgtcgcag gaccgtcact caccgcttca
     4201 ttctggagga ttcatggcca ttacagcaga tggctgacac ctgccgggat acggtcgtcc
     4261 tgctggaaaa aaacctcacc cgggtgatgc gcctgaaaaa acatccggta ccggaaaacg
     4321 ctgacgaaaa aaagaaacac acccgaaccc tgcaggatgc ggagcggtcc ctggcacagg
     4381 cccgcctgtc ggccaggcga ctggccctgc gtcatgtgga aaagtcacaa atcgtcacaa
     4441 cagatgcact cagcgaaaat gaaagtgaac tgctgcagcc tgaaggcccg ccctttcatc
     4501 tctgtgcctt ctgccacgcc tggcactgcc tgaacggcta cgctgccgca cagggggtga
     4561 tggtctggct ccctgatttg catccggcct cggtagttgc tctcaatgca cgggcactga
     4621 aggaaatttt cagcgatgag cgtaagcgaa tcagacaggg acgggccgta ctgaatgccc
     4681 tggtacagaa ccgcctggcc gttgaggaga aatttcgcac ctggcgtccg gcggattttg
     4741 ctgatgcact gcgccgctgg ccgccggcac agcgtaaaac actgcgggag aaaatggacg
     4801 gggtggcgct cattctgatg ccagattcgt ttcctgacaa aaagtacgtc atgtgacaca
     4861 caccgctgcc ggtgacgttc tgccggcccc aactgacaga ggaacaaaaa atggataccc
     4921 tcactgccgg atatcagttt tccgcagatg cattccggcg ctttattatc cggacgctga
     4981 ctgacgatat tccgcctgac ggagatgaaa aagagattgc ccggttcatt ctccgccacc
     5041 cggcatttaa cggcggctgg ctgctctttc tggctgcaga aattcatgaa agatcccgta
     5101 cccataacag catcaccgtc acagtgagcg tcgataacta tctctcatgg atggatattg
     5161 ccgtgtccgg tctcccggaa ttaccggata tgcccttctg tgattttttc attcgccgcc
     5221 agcagtttat ctatccgggt gtacccccga caaaatcagg agaagaatga tgaaaattca
     5281 ccgcatatcc ccggagacgc ttataacact catcctcgca cacctggccg gaaaagctga
     5341 cagtaccgcc aaagaagagc accggctact ccgtcggttc cttcgggatg atgacggcag
     5401 gctggccggc atactgctga atattgccgg aatacttcag ttcaacaggg aattaagcgc
     5461 ccggcataat tatccggcca cacccctgac tgaattttca ttaaggaaac ggggaaaaca
     5521 actacatctc tgtctctgca gtctccgttt cttttatatc ccccctgtat ttatacgaaa
     5581 tgaaagaaga aaatgtattg ttgtacatat taataaaatc acttatccgc cagtaaattc
     5641 actacgctga cagcgtgttt ccaccttatt acatttttat tattaactgt aatcacacag
     5701 aaatacgcac ctgtccctca acagattatt tattttctct gctgtttttt catgccacat
     5761 cccctgctgt ggcatcaaaa aatatcagac cgtgatgatt catccttacc ggagtgctcc
     5821 atgccatgaa tatcaaccgc attatttttg ccattgaaga ctgtatcagt accctgtcac
     5881 ggtattccgt ttccgcattc ttccccgatt actgtgatct gcacaccgtc atcggtctgg
     5941 accaacagga ccgcgaacgc cgcccctggc tgaaagcccc ctacattgcc accaccaaac
     6001 aggggtatta cctttccgtc tttgaaatca gcggtgccct gaaagaaatg gatgaaaacc
     6061 cggacctgac agcccccggc acactggaat cactgattac agcgctggca gagcgaatga
     6121 acactgccta taaaaacatc ggtcacaaaa tcagttttgt tttcgaacgc gatccggagc
     6181 gtggaaggaa tgaaatcgaa gtcatgctct taccccagcg aaaatccatc gcacgcacgg
     6241 ggatcctgct tcaggacgtt ctggatgaaa aagtcaccac gctcaccccc tggcttgtcc
     6301 gggaacgctg ctggctgacc gtctggagct ctgaagaact gctgagccgg accgatcgca
     6361 aggaccacca gacccgtatc aggaaactcg ctgaacacgc tccgccagcc cgatttgcgc
     6421 aggatccatg gcgctggaca ctttccgcac tcaaaatacg gcatgacgcc tttctggaca
     6481 cgctggagca ggccctgaca catgattctg atggtctgct ggttcgcctg atggatattc
     6541 atgaagtggg acgggaaatc cgccgtcaac tggagcgcaa cagcacacct gcagtatggc
     6601 agcctcatct gcctgaagat gcccgtcctg ccggctggcg acagggcgag gatacctctg
     6661 tattccacgc accgtcgctg aatctgcagt tattcaccac acagcctgaa acacacggca
     6721 gcctgataaa ggccggtgac ctctggcacg gtatggtcgc cattaccctg cccccccaga
     6781 atctgcggac attcaaccag ctggtcaggg atgtgccccg cgcaatcccc tggcgtatcc
     6841 ggatggatct gatgcccggc ggcatgaagg cgcttggggt caagaaaacc ctgctgacct
     6901 acagcagctt tatttcccct ctccggccca tgtatgactc agtgatgatg ctgaatgaca
     6961 tcaacaaaca tgatccggtc tgtatcatga ctattgttgc caccacatgg ggaaacagcc
     7021 gggaagtctg tacccgcaac caggcgctcc tgaaatctgc gctggaaggc tggggagtat
     7081 gcggcaccac caccaccttc ggtgatccga gacgtgcctg ggtgaacacg gttctggcgg
     7141 cctcccacgg ttccggtcca gtcccccttt acccgcccct ttcacacgcc ctttccctgt
     7201 ttcccctgaa cagggccggc tctgtctggc ggggacaggg aaatctgatg atgcacaccg
     7261 aagacggctc tgcatgggag gtcgcgctgg catcgtcaca acagaacaag cacacggaac
     7321 tgacgcccgg cgcacccggt ctgggcaaat cggtactcat caacgcactg tcggagatcc
     7381 agatagcctc agcccagaag aacctgcctt tcattgccta tatcgacaag ggattcagtg
     7441 cacagggact ggtacagctt atccgtgaca gcctgccgga gcagagaaag gacgaagccg
     7501 tggggatcat tctgagcaat gatccggaac acacccgtaa cctgtttgat gtgatgtacg
     7561 gtgctaaaaa acccgccacc ccggaaaaga acttcatggt atctgtactc tgcgcactgt
     7621 gcgtggatac cggcaccggt cagccctgta atcccggtga cacccggcaa atcataagca
     7681 acctggtgga tctggccttc agggagtatg gcgaaaacaa ccccagactg taccgtgcag
     7741 gaacagagcc ccttgtggat ctcgccctgg aagagagtgg catcgcagaa caacatgacg
     7801 ctggatggtg gaatgctgcc acctggtttg aaatcaggga catgctgcat atggctggca
     7861 atatccccgc agcccagcgg gcacactacc aggccatgcc cctgctggct gaaatgtccg
     7921 ccctgctggg acaaccatcc atccgggatg tgttcggcac agttcagcgc gataactcag
     7981 cagaattgtt actggactac atccgccgtg ccctcgatca gggacacagt gactacccga
     8041 tgatgtccgg ctgtacccgg tttatgctca gccccgacac ccgcgttgtg gccgttgatc
     8101 tgaacaatgt ggccggtgac aagacacctg ccgggcgtct gcgcactggc atcatgtacc
     8161 tgctggcagg acaaatcgcc ggcggggatt ttgtcctgcc gcagtatcag gaggaaatac
     8221 ggaaaaatct cgacccacgc taccatgagg tgattttccg ccgcattgaa cagcttgatc
     8281 aggaggtcaa gaccaaagtc tatgacgaac tgcacaatgc aaaagggatc aatttcatct
     8341 gggaagccct ggacacccag gagctggagc agcgtaagtt cgggatacgc acagtgctga
     8401 gcacccagct attgcaggac tatccaccgg cagttctcaa atccgccaac accctctggt
     8461 tgctgcgtta ccgccctgac gaaattccgt ttctgcggga taattttggt gtccctgaag
     8521 tcaccctgcg gcgcttcctg aaaatgccgg aaggggctgc accggatggc agcggtgtcc
     8581 cggtactggc tgtgttccgt gtaaaaaacg gtacgctggc ccggatcctg aaattcactc
     8641 tcggtccact ggagctgtgg gcactgaatt cctcacccaa agacagcgcc ctgagacgtg
     8701 cgctgacgca ggagctcggg tcactgcggg cacgccagat cctggcagaa aactttcccc
     8761 gtggttcagc cacctcactg atagaacacc gcgcccgtac acatgacagt gaaaacgtca
     8821 tccatgatct ggctgcagaa ctcatccgta agcagggata tcacctctga tgtacagcat
     8881 acacatcact ccccgctgcc ggcgtcctgc acggggacag gtcttcctga aaacgctgcg
     8941 cgtggacggt gacagtttca gcttcaccct gccgtccgcc tgcttcacgc tgcgtccctc
     9001 ccccaatccc cgggtcattt tcgccagtga cagacgggct gacggggaaa tgagtggcgt
     9061 tcgcctggct ctgcccggag agacaggcac gctgattaca acggccacct ggcaggtcaa
     9121 cgtgccggag cggcctcctt ttgatataca gcatgtcatt cactggctcc tgccctcgcc
     9181 tctcacggcg ctgatgtccg acaacacctg gctgtggcag gatagccccg gcacaccggc
     9241 actgacggcg ggagactggc cggtaatgga aaccgatacc ccgacgctga aacgcgtgaa
     9301 gcgtcgtacc ttcacgctca tcgataaccg caaccgtcag ggatggatca cccgtcgctt
     9361 ccagtgcttc ccgctggctc cgctgccgga atcacacagt caccgcgtac cagacatcct
     9421 gtatcccctg aaaacggaga aaaaagaatg caaagacaac cagcaaccac actgcttgca
     9481 ctcctgatag gctgtgcggt tatcccgtcc acccacgcct attctgtgac cgtagtggcc
     9541 agccagcctg tcaccacaca aatcatgccg gagctggccg ccattcaggg cgtgatggca
     9601 gagatggtcc gtatgcagac agccaccggc acagccatca accagagttc agagaaactg
     9661 gcctctgtca ttgcgcagga tgggcaggcc acccgtcagc agatgatatt cagtaatgaa
     9721 acccgccggc ttgaggaagc ccggaagagc ttcagcgtgc cggactccat ctgcagtgaa
     9781 tccgcctccg gagttgcagc tgaaagtcgc cgtgcagcag cgtctgcggc tgcacgtctg
     9841 agtcagggag gaggcgtcag cagtaaacca atccgtgaac gtctctcccg ggcagcagac
     9901 tcaccaatcc gcgaggcata tgacagtgca ggcattcatg ccggttactg cacggaagct
     9961 gaatatgccc gctttggcgg aaccgatgtc tgccctgccg tgggcgagct gccgggcgga
    10021 gacagccagg tgcgatcgct ctatcagggt gcaggcacag cagatacccc tgccgccctc
    10081 acctgggatc agaaacagat tgatgctgcc acggcttaca tgaaaaacac cgccagaccg
    10141 tcagcgggtc gtgccccggg gaaaggtgaa gtgggaacac agaccgggcg cacgtatgtg
    10201 ggcctgcaga atgaatacaa cggcatcatc gatgccgctt ctcatccgca actgtcactg
    10261 atagctgaca gtacccctaa tgaggccacc cgtggagcgc tgactgaagc cctgcagtca
    10321 ccatccgctg cggcatattt tgaccggacc gcatcatcag aggcccgcac ccgtggtcac
    10381 atgagccagc gggagtttga ggcctttgaa gctggcagac gctatgcaaa caccgactgg
    10441 cagcaggatc tgcagggcat ggaaggcgat aaccttctcc gtgaactgct gcgcactacc
    10501 gccctgctga actggcagat gaacgacctg aaagagcaga tacgccaggg taatgttatc
    10561 gccgggcagc agctggcact ggcggcacga cagtattacg gacagcgtct cggtgaactg
    10621 tcacaggcca tgagccaggg gagcgtaaga tgaaagacga caacagcacc gacaaaaaaa
    10681 aaccggcccc ccgtctccgc cgcatcggtg acatcgggat aaacctgttc gcaccactgg
    10741 cagaaacccg gtggatgctg cgcagctgcc gccatatgct gggccagcat aaaacgcgcc
    10801 tgaagcgcct catgcccgac cggcaaccgg agagtccggt ctgtatgacc tgggaagaag
    10861 ccgtcagggc cagcggcctt acggtcaacg aactggatcg gcgtctgcga cgtcgcagga
    10921 tgttctggcg gacaggattc tggctgatga tgataccggt acttctgctg tgcggcatgg
    10981 cccttgctgc cacagcgctt ccggccatca ccctgatacg tatactggcc tgcctgttac
    11041 tgatgagtgc cattgcagca ctgtgtgcct caaaaaccct gattgtgtca taccgcctct
    11101 ggcaactgaa tgaacggcgg gtcagcgagg acgaacaggg caccttcccc gattacctgc
    11161 aggcccggca cggctggaaa caggccacat ggctggccat tacccaccgg cagtactgac
    11221 cgtccggtct gcaattcctt ttcctcattc cggaggctga tgccctgcat cccttcggtt
    11281 ccggagttat cgtgaaaatc atacccagag cactgagtgc gggtctggcg ctggctgccg
    11341 tacctgcaca ggcattcgtc acctatcagg acattgtgag cgccgccacc aatccggatg
    11401 acctgtcgcg ccaggcactg gtcaccattt ttggcgacgt ggtcacaaat cccctgacag
    11461 ccagcagcaa caccattatc ggtaacctgt tcatggtgtt taacgcaatt atcgccatta
    11521 tggggatagt ctggttcctg tttattggaa tccgccatgt tgtccgcacc ggccaccagg
    11581 gcaatgtctt taacaacgga cgggacgtcg tcggaatgct cagtattgtg agcggtttta
    11641 tgatgattgt gcccaccgct tccggctgga gcctggcaca actggtcatg ctctggggcg
    11701 catccatcat gggcattggt tccgccaata tcatggttca gactgctgca gaaaacatcg
    11761 ctgaaggcta ttcaatgacc gtacagccag ttcatgtctc cacccgcacg gcagcacggg
    11821 gaatactgga gatggcgctg tgcaaacacg ccatgaatgc aggcctcgat gactttaacc
    11881 gtactgcccg ttcatcaacg ccccccatga gcgaatcctc acggacagag aacggcaggt
    11941 ataccgtcac cgtttcaaat ggcagtgcca cctgcgggag tgtgagtctc accgtgaaag
    12001 ggaatggcag caacaaccag tccgcactgg actatttttt caatcccttc attgaaacgg
    12061 agtatcaggg tgtcctgacg gcacaacgct ccgccatgga taccatgctc cgtgacctgg
    12121 acagcgcagc acaggaattt gtgtctgcgt accggacccg acgcgacacg ggtaacggca
    12181 acctgccgga tattgaaagc cgtattcaga aggctgccaa ccggtatgaa gaaacggtgc
    12241 agaaggcgtt gccaacagat aacgcagaaa ctgagcgcag ggagcagctg aaatactacc
    12301 tgaccaccta cggctgggtt gccctggggg cctggtacca gacctttgcc accgccaacc
    12361 agcgactggc agaactggcc gaccgtgccc ccactgtgag tggagtttca tcgcagggcg
    12421 aagccggaga tactgaattc ctgaaggaag taaaaggagc ttaccaggca cagttgcaaa
    12481 actcaccata tacacctgca ctgggaacaa ccgcttcatc aactgaatac agaacagcag
    12541 aagctgctga acctaaaagc ataatcatga aagtatggtc gggtgtaaat tttgcagaca
    12601 aaattgcaac atccttatct ggaacaggaa ccacctcaga ccaactaaat cctctgataa
    12661 aaatgaaaaa tatcggtgat tacactatgg tcacaacaga aaccatctgg gcgggttata
    12721 ccaccgtcag ggtactttcc actgccggca gcaactctat ttttggtcga ctggtcaatg
    12781 ccgttagcgg cttatctgat gccgcaaagg cttttctgga agcagctgca ccaccgctct
    12841 acttcctgct gtttttgctg ttctgtgccg gtttttcgtt atccatttac ctgccgttta
    12901 ttccgtttat cttctggatg accggcatcg gtaactggat agtcagtgta ctgattggat
    12961 gcgccgccgg tcccctgtgg gctgccaccc atctggggac aaaccaggac cggggaagcc
    13021 gggctgctta cggttacatc tacctgatag acagcatgat cagaccactg ttgatggtgt
    13081 tcgggttctt ttttgcatcg gtcgccatcg tggctggcgg caccatactg aatgcgttgt
    13141 ttggcgcggc tctggttaat gtgcaggctg actcccttac agggttattc agtatcgcgg
    13201 gatttttgat gatttatgcc cgtgcctgta ccacgctggt tgcggccgtt tttgcgcttc
    13261 aggcttatct gcctgaccat gtaattaatt ttctgggcgg acgtgacggc gttaatatgc
    13321 tggggaatat ggtttcatcc gtcaaagata ttattgccgg tggcaacacc aatatacgca
    13381 gaacacctgg cgttcgtgaa gaaaaactga aaaacattaa tggagatgat aaagacggaa
    13441 taaaacaata aagtacagac atcaaaaaaa acagtattgc cacattcacc tgaagagatt
    13501 aaaatacatt caggtgaata tggaggttta ttatgaacac aacaaaaagc agatatcctt
    13561 cctggtgggc atattatcaa ctagcccgca atacttactt tatatttgga attccatgct
    13621 tattattcta tgcttttttc ggaacactaa ttgttcatga ctcaagctta tctcaaacaa
    13681 gagattttat ttttgcatat ggatgctggg cattaataat tctcccatta ctctggctat
    13741 atctgaaaac cagagcgaag aaaaagaaag tccagcaggt tgttcagcag ataaaagaaa
    13801 gcagtccctt tgcaccgacc aacgattacg agcaactgtc attctcgaag agttgttatt
    13861 tcgggatcga cattaaaaac ggcaccatgc tgtatgtgcg catttaccca aacaatgtca
    13921 tggatgtgat tggcctggat attcacaact tcacccgaac agttgctgaa gacggcaagc
    13981 tggaaatcca caccacctat gtgagtctgc cgatgatccc tcttgaaatc tccggcatct
    14041 cagcaaggac gctggccaac accatacaca ccatggctgc acgtggatat gagtacaaag
    14101 agcgtttccc gcagatgatc cggtatcggg ttaaagagtg ggaaaaagtt gccggtgttc
    14161 ctgttgcaga agtattttga ggtagctatc aactgttaga cgataatccg tcagccggtt
    14221 ttctctgaac cggctttttc ttttcaggca gaatgcctgt ctgccgtccc tgtatcagac
    14281 tgtaattagc acaatacggc aatttgccgt tcagtatatt caggaagtac aaatcatgcc
    14341 atctgcaaca caaactgccc gactggaagc aaggatcagc acagaactgc atgctatgct
    14401 gaaacgcgct gcagaaatcc aggtaaaaaa gggcgcattg aggtttgctt tcgcaacaat
    14461 ccggcggcag atttcctgcc gccgtgttgc ccattaacgt ttaacttcgt aggctaacgt
    14521 tgccacaagc actgtgtttc cctgctcgac acggaatccg caaagcgaat ccctcaccat
    14581 ccagacaaaa cacagtatcg tcacacagac gacaattaac attgttaaaa acgttcgttg
    14641 tggcatcgca gcccctccat tgcctttcgg ccgtcatcgg gctactataa tgttgtctag
    14701 gcattacagt gggccctggg ttgattgata aaaaaatcgc ctggggcttt ctgctgcatg
    14761 ccgtccacaa aacgctttca gcatacagcc tcaagcgccc gccgctattc taatcgattt
    14821 tcagtctgcc acaatcgttt tccacctaat gacatgttat gcacaacagt atttttacgg
    14881 gaaactacac ggcaaggatt cccggaacac gttcattatt tgatggttac agacatcatg
    14941 ttaaatccgg aagtaataat gacctgaaag gaaatactat gagtaacgaa tatcaaagca
    15001 ccagtataaa ccctgatgtt tttgaacaat ttcttaccct cctgaataca cactgtaaat
    15061 catcagaagc tgcacttgcc atgctgagta cactctgcta cgaactgatg aggaggagtg
    15121 ggatccattc actggacagc ctgatgctca acggagaagg tatattgata caattacagg
    15181 gggctgatat tcgcgaaaaa acaggtcagc atattcactg atccagggat gtgctgaaaa
    15241 cagtgtcagc ctgaaataaa cgttcaatgc ctcatacaca tgaggcattg agcacggtca
    15301 tcaataatct ttattatttt tctgattcag catttccggt cagtatcggc tcttctggca
    15361 aagacattac cggaacgtca cgttcaccat tgaatacaga aacactttca gcgaaatcat
    15421 tctgtaacaa agaaaaactt aacggcagat ttttggtttg tatccctgtg atccattgaa
    15481 aaatctcggg aaaacgacgt gcactgatat gcacatgatt gaggtggtgc tcagtaatat
    15541 cactggccat catcatgaga ggtacatcgt atcccgaacg caaacctggc gcatgatgca
    15601 caggggtgct gtctgaactg acggattgcc catgatcaga aaaatacagc atggcaaaat
    15661 catcaccatg ctctgtcgtc atgttataaa ttgcctgaag aaaccgatcc agtttaagca
    15721 gtgttgataa ataacaggat actttatgtc cgaataattc cctgaatggc tgtgcatcat
    15781 taaaaagtcg gtcacaggga ttagtgtgag accccagcat atgaataaaa atcacttttg
    15841 gagaagacga gttaacagct tccctgacat actgaagcat atcctcatca tcaatgcgaa
    15901 gggaagaaga gctcccttcc ttaagaaaat gttttttatc cgcattggcg gcgatgacag
    15961 aaatcagcgt atcatgttgc cctaaagagc cctgatttga tatccaccac gtctcatacc
    16021 cggcttttct ggcaagcgta acaatattat tacccggatt acctgtttca gtgtaatcaa
    16081 gcgtcagtgt tctgctgagt gaacctatcg tattcggcgc tgctgagaaa tacccattaa
    16141 taaatatacc cggagcagaa ttcagccatg gtgttgtcgg caaaggatac ccataaacag
    16201 aaagataatc ccgtctgaca ctttcaccaa ttacgataat tatggtttta tatttcttct
    16261 ccacactcac aatatcccat gagtctttct gattgttttt cagcgattca tatcgcatca
    16321 tttcttctct ggtttcagct atccctgtca ttacattcgt aaccatacgt tttggccatg
    16381 catttactga caatacgata aagaacaaca gaaagataaa tccccggtgg cttttatcac
    16441 gcttacgcca gaaccagaat gtaaaagcag caatgaccag cgaaacaata aaataaagaa
    16501 caggtatgcc tgtgataaac tcacctgcct catttatatt tgtctgcatt acagacgcaa
    16561 taaaactgtt gtttattttc ccgtaaatca tcccaaccgg agcatagaat gcacttaaca
    16621 aaagaaagat acagacacac gttgctgaaa taacattccg ccgccccaac aacatcatca
    16681 ggaacaatat aagcacattc cccagggcgt ttttcactgt atatccgcag caccatgtaa
    16741 caagtacaga aaacaacaga taaatcagtg actctttaca ccacaacata aaataaaact
    16801 gttttgctct ctcaaaagca tatttcgact tttctttaat acaagacacg ccagtcattt
    16861 tcctgtacaa acacagacga tcatttcaac gcatggatat ttatatgaaa cagttcacaa
    16921 aaatacccta ccctgtccag cagggtttac atattatcag gttctgtgtt tacagcaact
    16981 gctccccgaa ccagattatt cgcataaccc gatgaaggcc tcataaccat caaacactca
    17041 gaatacattt agttaacaaa aatatgtctt gccctcccgg aaaggcaaac atacgaaaga
    17101 gaaagcatgg ctataataat atcaatcaaa atatgctcaa tctttccggt tgccatacac
    17161 tcagcaccga caacataaag acaataaaca aaagtgatag catagtgtga tatataaaga
    17221 atataaatgc acagaagtaa tttcccgaca actcttttcc gtttaaagaa agaataaatg
    17281 aagaacaaac ataaaaccgc cagaaaaaga aatgtagcta tagatattat aaaatactcc
    17341 gtcacctgtg aattctcatc cagagatgaa tacagcgaat aatatgtggt tgtgagatta
    17401 agccttgtta tcaatatata aataactgat aatagtataa tataaagaat cagaccaaat
    17461 ctgtttttct gcgctccggt taatacttct gatttatctc gcataaaata tcctgtctgt
    17521 aatctgtccc ggtaaacaat ggtaaatttc agaatcagtg cgaatacggt aacaaagatt
    17581 cctcctgtgc tttaaagtgt gtaggcagaa accagtctgg tggttccggt cagggagtct
    17641 gatacctcaa cttcccgggt gactataacc atacctgact gacgcagtct ctgcacatca
    17701 gctgcaattc gctgagttcc cagccagaac aggccatcca gtgccgttac cagcaacccg
    17761 ctttccagcg ccagtttcag ccgtgccgtc ggggctttaa cctgcccggc gatatcagta
    17821 aaacaacgcg tcgtcatgcc atagcagtcc atccgccgta ttcgcgtcgc aagactgtag
    17881 ctgaaacaat ccggcacgcc ggataagtca ttctgcagac tgtatacaca tccccgctgc
    17941 cgcccgttga acagggcttt tttctgactg agcagccatt cccgacgcag agtgtcaatc
    18001 atctgcggtg tccgggttaa cagcagacga aacggcagct caaaactggt cacataatca
    18061 acattcagta atgctgtccg cagtcgctcc ctggcacctg ccccgctctc ccggcattca
    18121 cgcatgacat cagcccactg acgcatcagt cgtggagact cattcctggc cctgaactga
    18181 tatttctcaa tatgataatc aatacgttcc tgcatatcgc caccggatat ctgttgttta
    18241 cccagacaca gtgattagcc tacaggaata aatatcctcc gtgaagccag gtgcctcaga
    18301 agataaaaac atgtactttt ttcaacgtgt aatattaaca ccacctccac aggaaaatat
    18361 aaaatgataa aatatatatt cgcactcaca cttctgcttt ccgggtctgt ctgtgccgat
    18421 caaattcagg gactcgttat tcgtgtgctg gacggagata ctctggagat attacaggcc
    18481 cggcatcctg tgaggattcg tcttgttaat attgatgcac cggaaaagaa acagcctttt
    18541 gggcgctggt caatgaatca acttaaaagc cttgtggtaa atacgcccgt aaccgtgaca
    18601 tacacacaga cagaccgcta cgggcgtatt ctgggacggg tatataccga agacggcatg
    18661 gaagttaacc gctggctggt acagcatggt gcagcctggg tatatcctga tttcaacaca
    18721 gattacaccc tgccggcata ccagagagaa gccagaacaa tgaaacgggg tctgtgggca
    18781 gacaaaaaac ctgttccacc atggctgtgg cgaaaagaaa ataaaaatag ccacaataat
    18841 tataaataag acatctcaca gaaatatatt tcctgccgtt ttatttcccc tgccagtccc
    18901 caacgggaaa taacccacag aaattacaca gatgtgaata ataaacctgt tcgatgtatc
    18961 cgtcacaaaa gagttgatgt ttgactcaga agaaaatacg cctgaagtta aggaaggctt
    19021 cagcttcatg attgttctga catgaacata tatccctctg tagcgccctg cctttcataa
    19081 catttctatt acagcaacaa cgaaagacaa cagcaatgtc ggccttttac gtccacatga
    19141 atattccact tatccggaat tgtgacacag cccctgaata caggattgat tgccagtgcc
    19201 cacaaatggc acattttcag taagattctg aatgttacat tcccccgtca tgtgtggcac
    19261 aactgtcacc gggaaagaaa cggaaagcac atgaaaaaca tcaaaactca caccggactc
    19321 cttatccaca aagaacaaac gcggcgcgtt cgtcttcatg aaactccggc cgcctggtgc
    19381 cacacgcacc gggaatgcta cagcaaaacg accggtcgtc gctgcggcag ccctgagagt
    19441 ctcagcaggc tgattctgag cagcattcag cccatagaat gagatgaccc gtatgaatga
    19501 tggcagacag acgcctccgt cccctgaggc atggaaaatc cggtttctgg cgaacggtac
    19561 acccggcaca tggttttaca cggatgagca gccgtcacaa aagagtggct gcgggcacat
    19621 tgtgacgatg tggaagtgat ccccctgtgc cgtatgcatg acggtcccgt tttccgggaa
    19681 caccatatta atgagctcag cgccgcactg gccagaatgc tccatatgca ccacatactg
    19741 ctggaacgaa cccggtacag agaaaatatc catgatgcag aatgtattcg tgaaattcac
    19801 gaagctcccc tgcaggctat cggcatcctg gaaaaataca aacgcacaaa atcataaggc
    19861 agttctatgt ggatactgat tttctggctc tcatccccct ttaatgtctc ctccggcgat
    19921 gcccggcaac ccacactggc cgttcacagt caggagttcc gtactgaaaa agcctgtcgg
    19981 gatgccttca ccgcgctgaa aaaagaaaac gtgggagaat tcgctctccg gggcgtctgc
    20041 gtccccggcg gagaataaac cggagatgat gattagccac aggagtattc aatggcatac
    20101 cattccccgg at
//
LOCUS       DAZKJR010000094        19754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKJR010000094 DAZKJR010000000
VERSION     DAZKJR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.094"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..172
                     /locus_tag="ACHOWS_003412"
     CDS             <1..172
                     /locus_tag="ACHOWS_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFQ0048940.1"
                     /translation="MMPLSSVGISSVQHEEYYIVFGALSLKSSLEDILLEITSLVDNA
                     LDLAEITEEYSH"
     gene            191..889
                     /gene="yjfJ"
                     /locus_tag="ACHOWS_003413"
     CDS             191..889
                     /gene="yjfJ"
                     /locus_tag="ACHOWS_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFQ0048941.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            939..1598
                     /gene="yjfK"
                     /locus_tag="ACHOWS_003414"
     CDS             939..1598
                     /gene="yjfK"
                     /locus_tag="ACHOWS_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFQ0048942.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFLRALGVDIPLTSLHII
                     G"
     gene            1616..2014
                     /gene="yjfL"
                     /locus_tag="ACHOWS_003415"
     CDS             1616..2014
                     /gene="yjfL"
                     /locus_tag="ACHOWS_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFQ0048943.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2024..2662
                     /gene="yjfM"
                     /locus_tag="ACHOWS_003416"
     CDS             2024..2662
                     /gene="yjfM"
                     /locus_tag="ACHOWS_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFQ0048944.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2665..3828
                     /gene="yjfC"
                     /locus_tag="ACHOWS_003417"
     CDS             2665..3828
                     /gene="yjfC"
                     /locus_tag="ACHOWS_003417"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFQ0048945.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            3912..5537
                     /gene="aidB"
                     /locus_tag="ACHOWS_003418"
     CDS             3912..5537
                     /gene="aidB"
                     /locus_tag="ACHOWS_003418"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFQ0048946.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERMEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5645..5920)
                     /gene="yjfN"
                     /locus_tag="ACHOWS_003419"
     CDS             complement(5645..5920)
                     /gene="yjfN"
                     /locus_tag="ACHOWS_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFQ0048947.1"
                     /translation="MKQFLASSSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6069..6398)
                     /gene="bsmA"
                     /locus_tag="ACHOWS_003420"
     CDS             complement(6069..6398)
                     /gene="bsmA"
                     /locus_tag="ACHOWS_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFQ0048948.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6580..7329
                     /gene="yjfP"
                     /locus_tag="ACHOWS_003421"
     CDS             6580..7329
                     /gene="yjfP"
                     /locus_tag="ACHOWS_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0048949.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHSTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7326..8081)
                     /gene="ulaR"
                     /locus_tag="ACHOWS_003422"
     CDS             complement(7326..8081)
                     /gene="ulaR"
                     /locus_tag="ACHOWS_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFQ0048950.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8189..9253)
                     /gene="ulaG"
                     /locus_tag="ACHOWS_003423"
     CDS             complement(8189..9253)
                     /gene="ulaG"
                     /locus_tag="ACHOWS_003423"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0048951.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9608..11005
                     /gene="ulaA"
                     /locus_tag="ACHOWS_003424"
     CDS             9608..11005
                     /gene="ulaA"
                     /locus_tag="ACHOWS_003424"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0048952.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKFAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11021..11326
                     /gene="ulaB"
                     /locus_tag="ACHOWS_003425"
     CDS             11021..11326
                     /gene="ulaB"
                     /locus_tag="ACHOWS_003425"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFQ0048953.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11336..11800
                     /gene="ulaC"
                     /locus_tag="ACHOWS_003426"
     CDS             11336..11800
                     /gene="ulaC"
                     /locus_tag="ACHOWS_003426"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFQ0048954.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            11814..12464
                     /gene="ulaD"
                     /locus_tag="ACHOWS_003427"
     CDS             11814..12464
                     /gene="ulaD"
                     /locus_tag="ACHOWS_003427"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0048955.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12474..13328
                     /gene="ulaE"
                     /locus_tag="ACHOWS_003428"
     CDS             12474..13328
                     /gene="ulaE"
                     /locus_tag="ACHOWS_003428"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFQ0048956.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEISVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13328..14014
                     /gene="ulaF"
                     /locus_tag="ACHOWS_003429"
     CDS             13328..14014
                     /gene="ulaF"
                     /locus_tag="ACHOWS_003429"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFQ0048957.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14143..14418)
                     /gene="yjfY"
                     /locus_tag="ACHOWS_003430"
     CDS             complement(14143..14418)
                     /gene="yjfY"
                     /locus_tag="ACHOWS_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFQ0048958.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14746..15141
                     /gene="rpsF"
                     /locus_tag="ACHOWS_003431"
     CDS             14746..15141
                     /gene="rpsF"
                     /locus_tag="ACHOWS_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0048959.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15148..15462
                     /gene="priB"
                     /locus_tag="ACHOWS_003432"
     CDS             15148..15462
                     /gene="priB"
                     /locus_tag="ACHOWS_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFQ0048960.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15467..15694
                     /gene="rpsR"
                     /locus_tag="ACHOWS_003433"
     CDS             15467..15694
                     /gene="rpsR"
                     /locus_tag="ACHOWS_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0048961.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15736..16185
                     /gene="rplI"
                     /locus_tag="ACHOWS_003434"
     CDS             15736..16185
                     /gene="rplI"
                     /locus_tag="ACHOWS_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0048962.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            16587..17078
                     /locus_tag="ACHOWS_003435"
     CDS             16587..17078
                     /locus_tag="ACHOWS_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0048963.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     ASSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            17081..18349
                     /locus_tag="ACHOWS_003436"
     CDS             17081..18349
                     /locus_tag="ACHOWS_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFQ0048964.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMAVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGFILIPGK
                     GWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRASI"
     gene            complement(18492..>19754)
                     /locus_tag="ACHOWS_003437"
     CDS             complement(18492..>19754)
                     /locus_tag="ACHOWS_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0048965.1"
                     /translation="NYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITKEQYSW
                     IVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLAFMRAL
                     MGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQF
                     AFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHI
                     LSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCL
                     ASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQ
                     CLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALA
                     FFDIIGAIALWMLIKVKDEEPQVQLATS"
BASE COUNT         4847 a         4644 c         5231 g         5032 t
ORIGIN      
        1 gatgatgccg ttgtcatcgg tagggatctc cagcgtgcaa catgaagagt attacattgt
       61 tttcggtgcg ttatcgctta aatcttctct tgaagatatc ctgcttgaga taacctcgct
      121 ggtagataac gcattggatc tggctgaaat tacagaagaa tattcacact aattacaagg
      181 acgggtattt atgggaattt taaaaagttt atttacgctg gggaaatcgt ttatctccca
      241 ggcggaagaa tccattgaag aaacccaggg cgtgcgcatg ctggaacagc atattcgtga
      301 cgctaaagct gaactcgata aagccggaaa atctcgcgtt gatctgctgg cgcgggtgaa
      361 attaagtcac gataagctga aagatttacg tgagcgcaaa gccagtctgg aagctcgtgc
      421 gctggaagcg ttaagcaaga acgttaatcc gtcgttgatt aacgaagttg ctgaagaaat
      481 cgcgcgcctt gagaatctca ttaccgctga agagcaagtg ttgtcgaatc tggaagtctc
      541 tcgtgatggc gtggaaaaag cagttacagc gacagcgcag cgtatcgctc agtttgagca
      601 gcaaatggaa gtcgttaaag ctactgaagc catgcagcgt gcacaacagg ctgtaacgac
      661 ctctactgtt ggcgcatctt ccagcgtttc gacagcggca gaatccttaa aacgcctgca
      721 aacgcgtcag gccgaacgtc aggctcgcct ggatgctgcc gcacagttgg aaaaagtcgc
      781 agacggtcgc gatcttgatg aaaagctggc ggaagccgga attggcggta gcaataaaag
      841 tagcgcccag gatgtattag caagactgca acgccaacag ggcgagtaat tttttgccag
      901 cctcactgca aggctggcaa ctattttaag gataaaatat gtctggtttt ttccagcgtc
      961 tgtttggcaa ggataataag cctgctatcg ctcgcggtcc tctgggactt catctcaata
     1021 gtggctttac gctcgacacg ttagcgtttc gtttgctgga agatgaattg ctgattgcgc
     1081 tgccgggtga ggaatttacg gtagccgctg tcagccgcat cgatctgggc ggcggtagcc
     1141 agattttccg ctactacact tcaggcgatg agtttctgca aatcaatacc actggcggcg
     1201 aagatattga tgacattgat gatatcaagc tctttgtcta tgaagagagc tacggtatca
     1261 gtaaagagag tcactggcgc gaggcgatca atgccaaagc gatgggggca atgaccttaa
     1321 actggcagga aaaacgctgg cagcgatttt ttaatagcga agaaccggga aatatcgaac
     1381 ccgtctacat gctggaaaaa gtagaaaatc aaaaccatgc taaatgggag gtccataatt
     1441 ttaccatggg ctatcaacgg caagtgaccg aagatactta cgaatatctg ctgttaaatg
     1501 gtgaggaatc ttttaatgat ctcggcgagc cggagtggtt atttttgcgc gcgctggggg
     1561 tcgatatccc gctgacatca cttcatatta ttggttaatt acaaggacgt ttacgatgca
     1621 tatactggat tcacttcttg cctttagcgc ttattttttt attggtgtgg ctatggtgat
     1681 tatttttctg tttatctact ccaaaattac accgcacaac gaatggcagt taatcaaaaa
     1741 taataacacc gcggcggcac tggcgttcag cggaacattg ttggggtacg tgatcccctt
     1801 atccagtgcg gcaatcaatg cggtgagtat tccagactat ttcgcatggg gcgggattgc
     1861 tctggtgatt caattactcg tttttgctgg cgtcaggctt tatatgcccg cattaagcga
     1921 aaaaattatt aatcacaata ccgcagcagg aatgtttatg ggaaccgccg cgctggctgg
     1981 cggtattttt aacgcagctt gtatgacatg gtaatggacg atcatggcca gaaaacgcaa
     2041 atccagaaat aacaaaaata tcggacatgg ggcaatcagt cgcattggta gaccgcataa
     2101 tccttttgag ccgcgccgca atcgctacgc acaaaaatac ttaacgttgg cgctaatggg
     2161 cggtgccgct tttttcgtat tgaaaggttg tggcgatagt ggcgatgtcg ataacgacgg
     2221 cgacggaacg ttttacgcga ctgtgcagga ttgtattgat gacggtaata atgcagacat
     2281 ttgcgcgcgt ggctggaaca acgccaaagc ggcgttttat gccgatgttc cgaagaatat
     2341 gactcagcag aattgtcagt ctaagtacga aaattgctac tacgacaatg ttgaacagag
     2401 ttggatcccg gtcgtttctg gatttttgtt aagccgggtt attcgtaaag atcgcgatga
     2461 gccgttcgtc tataacagcg gcggatcctc atttgcttcg cgccccgtct ggcgcaacac
     2521 ttctggtgat tactcctggc gctccggttc tggcaaaaaa gagtcttact cttcgggcgg
     2581 ctttaccacc aaaaaagcgt caaccgtttc tcgcggcggc tatggtcgtt cttccagcgc
     2641 ccgtgggcat tggggaggct aatcatgctg agacacaacg ttcctgtgcg gcgggatctg
     2701 gaccagatcg ccgctgacaa cggtttcgac tttcatatca tcgacaatga aatctattgg
     2761 gacgagagtc gagcttaccg ttttactctg cgccagattg aagagcagat cgaaaaaccg
     2821 actgcggaac tgcatcagat gtgccttgag gtggtggatc gcgcggttaa agacgaagag
     2881 atcctgacgc aactggcgat cccgccgttg tactgggatg tgatcgccga aagctggcgc
     2941 gcacgcgatc cttcgttgta tggccgtatg gattttgcct ggtgtggtaa tgcaccggtt
     3001 aagttgctgg agtacaacgc cgatacgcca acttcattgt acgagtcggc ttatttccag
     3061 tggttgtggc tggaagatgc ccggcgcagc ggcgttattc cgcgcgatgc cgatcagtac
     3121 aatgccattc aggagcgcct gatttcacgc tttagcgagc tttacagtcg ggaaccgttt
     3181 tatttttgct gctgtcagga caccgacgaa gacaggagta ccgtgctgta cttgcaggac
     3241 tgcgccgagc aggcagggca ggagtcgcgg tttatctaca ttgaagatct cggtttgggc
     3301 gtcggcggtg tgctgaccga tcttgatgat aatgtcatcc agcgtgcatt taagctgtat
     3361 ccgctggagt ggatgatgcg tgacgataac ggtccgctgc tgcgcaagcg tcgcgagcaa
     3421 tgggtggagc cgttatggaa aagtatcttg agtaataaag ggctaatgcc gctgctttgg
     3481 cgcttcttcc ctggtcatcc taaccttctt gcatcctggt tcgatggtga aaaaccgcag
     3541 attgccgctg gcgaaagcta tgtgcgtaaa ccgatttact cgcgcgaagg cggaaacgtc
     3601 accattttcg acggtcagaa taacgttgtt gaccacgctg atggtgatta cgccgatgaa
     3661 ccgatgatct accaggcgtt tcaacctctg ccgcgatttg gcgatagcta cacactcatc
     3721 ggtagctgga ttgtcgatga tgaagcgtgc ggaatgggga tccgtgaaga taacacgttg
     3781 atcaccaaag acacctcacg tttcgttccg cattacattg ctggataaga atgttttagc
     3841 aatctctttc tgtcatgaat ccatggcagt gaccatacta atggtgactg ccattgatgg
     3901 agggagacac agtgcactgg caaactcaca ccgtttttaa tcaacctata ccattaaata
     3961 acagcaactt atacctgtct gatggcgcgc tctgcgaagc ggtaacgcgt gaaggtgctg
     4021 gctgggatag cgattttctc gccagtattg gtcagcagtt agggacggct gaatcccttg
     4081 aactggggcg gctggcgaat gtgaatccgc ctgaattatt gcgctacgat gcccaggggc
     4141 ggcgtctgga cgatgtgcgt tttcaccccg cctggcacct gctgatgcag gcgctatgta
     4201 ccaatcgggt gcacaatctt gcctgggaag aagacgctcg ctccggcgca tttgtggcgc
     4261 gcgcggcgcg ttttatgttg cacgcacagg ttgaggcagg gtcgttatgt ccgataacga
     4321 tgacctttgc cgccacgcca ttgctgttac agatgttacc cgcgccgttt caggactgga
     4381 ccacgccgct gttgagcgat cgctacgatt ctcacttatt gccaggtggg caaaaacgcg
     4441 gtttgttgat tggcatggga atgacggaaa aacagggcgg ttccgatgtc atgagcaaca
     4501 ccacccgcgc agagcgcatg gaagatggct cttatcggct ggtggggcat aaatggtttt
     4561 tctcggtgcc gcaaagcgat gcgcatctgg tgctggcgca gactacgggc ggtttgtcct
     4621 gcttttttgt gccgcgcttt ttgcctgacg ggcaacgcaa cgcgattcgc ctcgagcggc
     4681 tgaaagataa gctgggtaat cgctctaacg ccagctgcga agtggagttt caggatgcca
     4741 ttggctggtt gttggggcag gaaggggaag gaattcgtct gatcctgaaa atgggtggga
     4801 tgacgcgttt tgattgcgcc ctgggtagcc atgccatgat gcgccgtgca ttttcgctag
     4861 cgatttatca tgcacatcaa cgccatgttt ttggtaatcc attgatccaa cagcccctta
     4921 tgcgtcatgt cttaagtcgc atggcacttc agcttgaagg gcaaacggcg ttgctgtttc
     4981 gtcttgctcg agcgtgggac cggcgtgccg atgccaaaga agccctgtgg gcgcgtttat
     5041 ttacgcctgc ggcgaaattt gtgatctgca aacgaggtat gccgtttgtg gccgaagcga
     5101 tggaggtgct gggcggcatt ggttattgcg aggagagcga gctgccgcgg ctttaccggg
     5161 agatgccggt aaacagtatt tgggaaggtt ccggtaatat tatgtgcctg gatgtactgc
     5221 gcgtgctcaa taagcaagcg ggtgtatacg acttattgtc ggaagcattt gtggaagtga
     5281 aagggcagga tcgctatttt gatcgcgcgg ttcgtcgttt acagcagcag ctgcgtaagc
     5341 cagctgaaga actggggcga gagattactc atcagctatt cctgctgggc tgcggtgcgc
     5401 aaatgttgaa atatgcctct ccgccaatgg cgcaggcgtg gtgtcaggtg atgttagata
     5461 cgcgcggcgg cgtacggttg tcagagcaga tccagaatga tttattgctg cgggcgacgg
     5521 gaggagtgtg tttgtaagcg tatacgacgc tggtttcgat taactcaatg aaatatgtga
     5581 aaattgttag ccggacaagg cgctcgcgcc gcatccggca ttgttcattt gctgtagatg
     5641 acactcacgc atacaggatc gcctgtacgc gccagttatc aggctgctgg tcttcgtaca
     5701 tggtaataat gcggtaccat gacgcacctt gttcatcagc ctttaacgcc acgatacgtt
     5761 ccacgtcctg cggatcccct gaaatattgc taactgagat ctgacctatt tcattcaggc
     5821 ctgtcacgca gtcagcactg gcgaattctg cagactgtgc cgtggcgctc gcaggataag
     5881 tcactaattg cagcgaggat gaggcaagga attgtttcat cgtcagctcc atttttcgtt
     5941 tccccacatg ttgcagggca ttccatcgcg gtaataaacg cccaggcttc cagccggtaa
     6001 tttattgtgc acaggtaaac gcatagggac gcaaagcaat gtaaagctga ctatgtaagt
     6061 tgtctgattt atttacggta caaaatggct tgtgaatacc actgtcctgt gacgatggtt
     6121 tcgtccacca tgacgacaac gtaataatca gcttttgcag cgacagcttt cgctctgatt
     6181 tctgctaatg cgtcatccgg agaaccccga accatcgtgc ttacgctacc tattcgctgt
     6241 aacccttgcg tctggtcgcg gcgaatctct tgcggatggt cggttactgg cggtgctggc
     6301 tgtggcgtac cttgtagtgc gctacaggca cttaacatca acaccaataa taaactggca
     6361 aaccggtaaa taacgctatt acgtttcctg ctaaccatag tgtagtgcct tattaattta
     6421 actttggggg aatgttcccg aattgttacc tggtacgcga ttttcgaatg aaaacgtgat
     6481 gaaagcgtaa cccacatctc aacattatga actaataccg ccagcagaaa cttgtgctaa
     6541 ggatttcttc atgtcatacc agacaaaaag gagagacaga tgattgaaat agaatcacgc
     6601 gagctggcag atattcccgt tcttcatgct tatcctgtcg ggcaaaaaga taccccgtta
     6661 ccgtgcgcga ttttttatca cggctttact tcgtccagtc tggtgtatag ctattttgcc
     6721 gttgcgctgg cgcaggctgg tttgcgggtg atcatgccgg atgcgcccga tcacggtagt
     6781 cgttttagtg gcgacgcagc gcggcggtta aatcaattct ggcaaatctt gctacaaagt
     6841 atgcaggaat tcactacttt acgtgcggca atagccgaag aaaactggct gcgtgatgac
     6901 cgtctggcag tcggtggcgc gtcgatgggc gcgatgacgg cactgggaat taccgctcgc
     6961 cactccacgg tgaaatgtac cgccagcatg atgggatcgg gctattttac atcgctcgcc
     7021 cgttcactgt ttccaccgct gatacctgaa acggcagcac agcagaatga attcaataac
     7081 attgtcgcgc cgctggcaga gtgggaagcg acaaaccacc tggaacaact tgctgacaga
     7141 cctctactgc tgtggcatgg ccttgacgat gacgtcgtgc ctgccgacga atcactacgt
     7201 ttgcagcagg ccttaagcga gacgggacgg gataaactgc taacctgttc atggcagcca
     7261 ggcgtgcgtc accgcattac gcctgaggcg ttagatgctg ccgtgacatt tttccgccag
     7321 catctttaaa cacgcagaat gctgacccct tgcgcttcca gttgttgcag gatttccggg
     7381 ttagcatttt tgccggtgat cagcatatcg atttgatcgg cacggctaaa aagcattccc
     7441 gcgcgttcgc cgatcttact gctatcaacc agtaccacca gtttccctac cacgcttagc
     7501 atcttctgct ctgccattgc cgtcagcata tcggttttat acaacccttc tgctgtcagc
     7561 cctttgccgc tggtaaacat ccagtgcccg gcatagagac tgttttcgct gccttgcggg
     7621 cttaaagtga tggactgact tttgttgtac tgtccgccca taatgatcac gctgtcatgc
     7681 tcctgatcga tcaggtaatt tgccagcggt agataattag tgatgatttg cactggcttg
     7741 ccacacattt cccgcccaag cagaaacgcg gtggagccgc agttgatgac tacgctttcg
     7801 cccggattaa ccagctgcga ggccgcttta gcaatacgta ctttttcatc gtgattctgc
     7861 gcctgatgca gattcatcgg cgtccagcgt gggcgctgct gggtaatagc ttctgcgcca
     7921 ttgcgcactt ttttcagttt gccgctttcg tcaagtttat tgatatcgcg tcgcgcagtg
     7981 gcaggcgaaa ttcccagacg ctcaacgact ttctcaacgg tcacaaagcc caattgtgcg
     8041 agcatttcca ggaggatttg atgtctttgt gcttcagtca tgagctattc cgataaaatt
     8101 tgattttttt agatgatatt tgaaatagcc aggaaatact acgccggata gcgcgaacta
     8161 ctccacgcta tccggtacaa gcgacagatt acaggaacga cttgaacggc agatccggtt
     8221 caatagtgaa gcaatcatcg aaaccacgcg gatagtggta ctcgaagttg tctttatcca
     8281 gcggccaggt aaatttgccg ccgacctgcc agataaacgg cttgaagcca tacttcaggc
     8341 gatctttttt catctcccac agcacgcgga tctcttgcgg atcggcctgg aagtttgacc
     8401 agatatcgtg gtggaacggg atcactactt tagcattcag cgcttcgccc atacgcaaga
     8461 tatcagcact ggtcattttg tcggtaatac cgcgtgggtt ttcgccgtat gaacctaaag
     8521 ccacgtcgat ctgatgctcg ttgccatgtt ttgcatagta gttagagtag tgggaatcgc
     8581 cgctgtgata cagagaaccg cccggtgttt tgaacagata gtttactgcg cgatcgtcca
     8641 tgccgtctgg cagcacgcca gccgcttttt gatccgctgg tagggtgatc agtgcagtac
     8701 ggtcgaacgc gtcaagggca tgaatttcaa tgtctttcac ttttacgaca tcgcccggtt
     8761 tgaccacgat gcaacgctct ttcggtacgc cccagccaat ccataaatcc acgcaggttt
     8821 tcggtccgat aaacggtacg tcatcagcac agttctgcat cacggcagca gcgacgttga
     8881 cgtcgatatg atcgttgtga tcgtgagttg ccagtaccgc gtcgatctgg cgaatcgcaa
     8941 acggatcaag aacaaacggg gtggtgcgca ggtttggctg cagttttttc acgccagcca
     9001 tgcgctgcat ctggtgaccc tgtttcatca gcgggttacc gtgactttgt ttgccagtac
     9061 cgcaccagaa atcaacgcaa acgttggtgc caccttccga tttcagccag atcccggtgc
     9121 agcctagcca ccacatcgcg aatgtgccag gggcgacctg ctcttgttca atttcttcat
     9181 tcagccagct accccactcc gggaaagtgc tcaggatcca ggattctcgg gtgatacttt
     9241 tcactttact catcgccatt taccttcatg atagttcaat tcgaatcaac atgtgattgg
     9301 ttttgattaa tcctgacact attttttcag gaaggcaatg accatttttt gactttttgc
     9361 cagggaagtt gttgttgatt ttgagtgtgg aaagatttaa tggaatgtgt aattcaatta
     9421 actaaatgaa tttaaatgaa taattatttc gttgtgtgaa tcccactcta tccatgtgga
     9481 attatttgcg ggtcgcgtca catttaatca taaataatct tgttgtgatt acttttgaaa
     9541 attagagtga gtgcacaaca ttccgggtgt gtggaatacc cggttacctc ttcttcagga
     9601 gatcgttatg gagatcctct acaacatctt taccgtgttt tttaaccagg tcatgaccaa
     9661 tgccccgttg ttgctgggta ttgtgacctg tctgggctac atcctactgc gcaaaagtgt
     9721 cagcgttatt attaaaggca cgattaaaac cataattggt ttcatgttgt tgcaggcagg
     9781 atccggcatc ctcaccagca ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg
     9841 cattaacggc gcaatttccg atacttacgc gtcgatgatg gcaaccatcg accgcatggg
     9901 cgatgcctat agctgggtag gttacgcggt gttgttagcg ctggcactga acatctgtta
     9961 cgtgctgctg cgtcgcatta ccggtattcg taccatcatg ctgactggtc acatcatgtt
    10021 ccagcaggcg gggctgattg ccgtcacgct gttcatcttt ggctactcca tgtggacgac
    10081 cattatctgc accgcgattc tggtttcgct ctactggggc attacctcca acatgatgta
    10141 caagccgact caggaagtga cggacggctg cggtttctcc atcggtcacc agcaacagtt
    10201 tgcatcatgg attgcctata aatttgcgcc gttcctcggc aaaaaagagg aaagcgttga
    10261 agacctcaaa ctgccaggct ggctgaatat tttccacgac aacatcgtct ccacggcgat
    10321 tgtcatgacc atcttctttg gtgccattct gctctccttc ggtatcgaca ccgtgcaggc
    10381 gatggcaggc aaagtgaact ggacggtgta catcctgcaa actggtttct ccttcgcggt
    10441 ggcgatcttc atcatcactc agggtgtgcg catgtttgtg gcggaactct ctgaagcgtt
    10501 taacggtatc tcccagcgtc tgatccctgg tgcggttctg gcgattgact gtgcggctat
    10561 ttatagcttc gcgccgaacg ccgtggtctg gggctttatg tggggcacca tcggtcagct
    10621 gattgcggtt ggcatcctgg tcgcctgcgg ttcctcgatc ctgattatcc caggctttat
    10681 cccgatgttc ttctccaacg ccactattgg cgtgttcgct aaccacttcg gcggctggcg
    10741 tgcggcgctg aagatttgtc tggtgatggg gatgatcgaa atctttggct gcgtctgggc
    10801 ggtgaaactt accggtatga gtgcctggat gggtatggcg gactggtcga ttctggcacc
    10861 gccgatgatg cagggcttct tctccatcgg tatcgccttt atggctgtca tcattgtaat
    10921 tgcactggct tatatgttct tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa
    10981 acaactggca gaacagtctg cttaataagg agttttgatt atgaccgtac gtattctggc
    11041 tgtgtgtggc aacggacaag gcagttccat gatcatgaaa atgaaagtgg accagttttt
    11101 aacccagtcg aacattgacc acacggtaaa cagctgcgcg gttggcgaat acaaaagcga
    11161 gttgagcggc gcggatatca tcatcgcttc tacccatatt gcgggcgaaa tcactgtgac
    11221 cggcaacaaa tatgtggttg gcgtgcgcaa tatgctctct cctgccgact ttggcccgaa
    11281 attgctggaa gtgatcaaag agcattttcc gcaggacgtg aagtaaggac gcgccatgaa
    11341 attacgtgat tcgctggcgg agaataaatc catccgcctg caggctgaag ctgagacatg
    11401 gcaggacgca gtaaaaatcg gcgttgacct gctggtggcg gcggatgttg tcgagccgcg
    11461 ttactaccag gcgattctgg atgcggttga acagcacggt ccgtactttg tgctcgcacc
    11521 cggcctggca atgccgcacg gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct
    11581 ggtgacgctg aaaaagccgc tggagttcaa ccacgaagat aacgatccgg tggatatcct
    11641 catcaccatg gcggcggtcg atgccaacac tcaccaggaa gtgggcatca tgcagatcgt
    11701 caatctgttt gaagatgaag agaatttcga ccgtttacgt gcctgccgta ccgagcagga
    11761 agtactggat ctcattgacc gcaccaacgc ggcagcttaa gaaggaattg aaaatgtcat
    11821 taccgatgtt gcaggtcgcg ctggacaacc agactatgga tagcgcctat gaaaccactc
    11881 gcctgattgc cgaagaagtc gacattatcg aagtgggtac cattctgtgc gtgggcgaag
    11941 gcgtgcgtgc ggttcgcgat ctgaaagcgc tttacccgca caaaatcgta ctggcagacg
    12001 ccaaaattgc cgatgcgggc aaaatccttt ctcgtatgtg cttcgaagct aacgctgact
    12061 gggtgaccgt gatttgctgt gcggatatca acacagctaa aggtgcgctg gacgtggcaa
    12121 aagagtttaa cggcgacgtg cagatcgaac tgaccggtta ctggacctgg gaacaggcgc
    12181 aacagtggcg cgacgcaggc attgggcagg tggtttatca ccgcagccgt gacgcacagg
    12241 ccgcaggtgt ggcgtggggc gaagcggaca ttaccgcgat caaacgtctt tccgatatgg
    12301 gcttcaaagt caccgtcacc ggtggtctgg cgctggaaga tctgccgttg tttaaaggca
    12361 ttccgattca tgtgtttatc gctggtcgca gcattcgtga tgccgcttct ccggtggagg
    12421 ccgcacgtca gttcaaacgt tccatcgctg aactgtgggg ctaaggagcg gatatgttgt
    12481 ccaaacaaat cccgcttggc atctatgaaa aagcgctccc cgccggggag tgctggctgg
    12541 aacggctgca actggcaaaa acgttaggct tcgattttgt cgagatttcg gtagatgaaa
    12601 cggacgatcg cctgtcgcgc ctcgactgga gccgcgagca gcgtctggcg ctggtgaacg
    12661 cgattgttga aaccggcgtg cgcgtgccgt ccatgtgcct ttctgctcat cgtcgtttcc
    12721 cgctgggcag cgaagatgac gcagtgcggg cgcaggggct ggagattatg cgcaaagcta
    12781 tccagttcgc ccaggatgtc ggtattcgcg tgatccaact ggcgggctac gacgtttact
    12841 atcaggaagc caataacgaa acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg
    12901 agatggcaag ccgcgcgcag gtgacgctgg cgatggagat catggattac ccgttgatga
    12961 actccatcag caaggcgctg ggttacgcgc actatcttaa caacccgtgg ttccagctct
    13021 acccggatat cggcaacttg tcggcgtggg acaacgacgt gcagatggag ttgcaggccg
    13081 ggatcgggca tatcgtcgcg gtgcatgtga aagacaccaa accaggcgtc ttcaaaaacg
    13141 tcccgtttgg cgaaggtgta gtggatttcg aacgttgctt cgaaacgctc aaacagagtg
    13201 gttattgcgg gccgtacctg attgagatgt ggagcgaaac ggcggaagac ccggcggcag
    13261 aagtggcgaa agcgcgtgat tgggtgaaag cgcgcatggc caaagccggc atggtggagg
    13321 cggcataatg caaaagctaa aacagcaggt atttgaagcc aatatggatc tgccgcgcta
    13381 cgggctggtg acctttacct ggggcaacgt cagcgccatt gaccgcgaac gcgggctggt
    13441 ggtgatcaag cccagcggcg ttgcctacga aaccatgaaa gcagacgata tggtggtggt
    13501 tgatatgagc ggcaaggtgg tggaagggga gtatcgtccg tcttccgaca ccgcgacgca
    13561 tcttgaactc taccgccgtt atccgtcgct tggcggcatt gtccataccc attccacgca
    13621 tgccaccgca tgggcgcagg cggggctggc tatcccggca ttaggcacca cgcacgctga
    13681 ctacttcttt ggcgatatcc cctgtacgcg cgggttaagc gaagaagaag tgcaggggga
    13741 atacgagcta aacactggca aagtgattat cgaaacgctg ggtgacgccg agccgctgca
    13801 tacgccggga attgtggtgt atcagcacgg gccattcgcc tgggggaaag atgctcacga
    13861 tgcggtgcat aacgcggtgg tgatggaaga agtggcgaaa atggcgtgga ttgcccgcgg
    13921 cattaacccg caactgaatc acatcgacag ctatctcatg aataaacact tcatgcgtaa
    13981 gcacggtccc aacgcctatt acgggcagaa gtaaaacacg cgctgcggag atttccttct
    14041 tcgggagata actggtctaa gtccgcagcc gtttttcaaa aaaagccccc tgaaaagggg
    14101 gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg tgctagcgat aaatatccgc
    14161 gctggcgtgc atattgccgt tactccccgg ttcccgcatc agaatcacgt ggtagtacgt
    14221 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    14281 attatgattg atataaatca cgcctaagct ttgcacatcg tccatatttc tggcctggtg
    14341 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    14401 taaaacacgg ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctcttgt
    14461 ggtgatcttc tgatttaatt ttaatcaatg ataaagaagt ggatggtgac catttctgat
    14521 gcagttgttc aaaaaaacac catgatgaag tgtgatgaac ttcaaatcag cgtgttagta
    14581 cgatttggtg aaggatgaga tttatttgtc ggctggcctt gagtttagcg gggtatgtca
    14641 gtactataac gcgtcatttt tcagccgacc tttaacacgt tccttgcctc cccgggattc
    14701 ggctgaccca gacaggaggc tgaataatcc gtaaggagca attcgatgcg tcattacgaa
    14761 atcgttttta tggtccatcc tgaccagagc gaacaggttc cgggcatgat cgagcgctac
    14821 actgctgcca tcactggtgc agaaggcaag atccaccgtc tggaagactg gggccgccgt
    14881 cagctggctt acccgatcaa caaactgcac aaagcacact acgttctgat gaacgttgaa
    14941 gctccgcagg aagtgatcga tgagctggaa actaccttcc gcttcaacga tgccgttatc
    15001 cgcagcatgg ttatgcgtac caagcacgct gttaccgaag catctccgat ggttaaagcg
    15061 aaagacgagc gccgtgagcg tcgcgatgat ttcgcaaacg aaaccgctga tgatgctgaa
    15121 gctggggatt ctgaagagta atttctgatg accaaccgtc tggtgttgtc cggcaccgtg
    15181 tgcaggactc cccttcgaaa ggtcagtcca tcaggaattc ctcactgcca gttcgtgctt
    15241 gagcatcgtt ctgtgcagga ggaagccggc tttcaccggc aggcatggtg ccaaatgccc
    15301 gttattgtta gcggacacga aaaccaggcc attactcaca gtataacggt cggcagtcgc
    15361 ataaccgttc aggggttcat ttcatgccat aaggcaaaga acggactgag caaaatggtt
    15421 ttgcatgccg agcagattga attgatagat tctggagact agccatatgg cacgttattt
    15481 ccgtcgtcgc aagttctgcc gtttcaccgc ggaaggcgtt caagagatcg actataaaga
    15541 tatcgctacg ctgaaaaact acatcaccga aagcggtaag attgtcccaa gccgtatcac
    15601 cggtacccgt gcaaaatacc agcgtcagct ggctcgcgct atcaaacgcg ctcgctacct
    15661 gtccctgctg ccgtacactg atcgccatca gtaatcggtc acggtccatt aatacgactt
    15721 tgagaggata aggtaatgca agttattctg cttgataaag tagcaaacct gggtagcctg
    15781 ggtgatcagg taaacgttaa agcgggctat gctcgtaact tcctggtacc gcagggtaaa
    15841 gctgttccag ctaccaagaa aaacattgaa ttcttcgaag cacgtcgcgc tgaactggaa
    15901 gctaaactgg ctgaagttct ggcagcagct aatgctcgcg ctgagaaaat caatgcactg
    15961 gaaactgtta ccatcgcgtc taaagctggc gacgaaggta aactgttcgg ttccatcggt
    16021 actcgcgaca tcgctgacgc tgtaactgca gctggcgttg aagtggctaa gagcgaagtt
    16081 cgtctgccga acggcgttct gcgtaccact ggcgaacacg aagtgagctt ccaggttcac
    16141 agcgaagtat tcgcgaaagt gatcgtaaac gtagtagctg aataattcgt tattcaacga
    16201 gacgtaaaaa cgccgaccag tggtcggcgt tttgctttta agtgataaaa accgcagaaa
    16261 cagacgttcc ctccataaat ctcttgctat tccttttata aaaagttcgc agtacccata
    16321 cctggccccc agcctcgccg ctggtagttc gtgtcatgtt ttttacgcgt aaaacacgac
    16381 aaaagcatca atacagcgat cgtctcactt gtatgataat aaaaacaata tgttatatat
    16441 gaatgtgctg ttattttatt gtttttatat ataaaaagat aaaataccat cactttcgta
    16501 atcttgaacg ctttgcaggt gcgttcaatc caataaatgc taatattatg gctatcatag
    16561 ccataaaaag ataaaatatt atcactatgg gtaaaaaaac agcaccgtta ctcccttata
    16621 cgaatcagat gcttagtgaa tttggtgagc ggctgaaact tgctcgttta cgacgcaggc
    16681 ttaccgcaaa gcaagttgct gaacgtgctg ggatgtcgcc aataacattg cgctctctgg
    16741 agtcaggaag tgcaggggtc acgatagggg catatttgtc tgtgatgcag gttttgggcc
    16801 tggaaacgga tttaaataag cttgcagctg aagacgactt agggcgtcag ctccaggatt
    16861 cccgtttgat taaaaaaaga gggctttctc gtgcatcaag taagaatgaa gaaggttctc
    16921 agacagcctc attgcagatg aggcacgaga aagaaggtca tctcaccacg gatttaacag
    16981 cactggcaga aggctcgacg ccatctatta caggagaaac atcaacctct cttgcagcac
    17041 tattaaaacc gataaaaaaa ccgttaaaag gaaattaatt atggcattaa tcggagtgta
    17101 tgcggattgg gaaggcctgg atggtccgga gcggattggt tatttacaca gtcgccgcac
    17161 tcggacgcgt gagatatttg agtttgagta cgataaaaag gcgcttgcag atccttcact
    17221 taatttcatt cagttagatc ctgaaataat gttgtatgag ggagcacagt acccaattcc
    17281 tccaaaggat aaatttggtg catttagtga ttcctgcccg gatcgttggg ggcgtatgct
    17341 aatgaagcgt cgttttgaga gggatattcg agacgggtta tgtgataaag actcccacct
    17401 ttatgagtct gattatctac taggtgttca tgatctttat cgggttgggg cccttcgata
    17461 caaacgtgaa gacgctggag agtttcttga taatcgcatt gatgttgcag ccccaccatt
    17521 taccgaaata gcgagtctgg aaagggctag ccgggcaatt gaagaagatc ctgataataa
    17581 agagctaatg gggcaaaaat ggttaagaat gctaatcgct ccggggggat cattaggggg
    17641 ggcacgtccg aaagccagtg ttgttgatga agctgggcat ctctacatcg ctaaatttcc
    17701 cagtgtaaaa gatgagtatg atgttggtgg atgggaaatg gccgttaatg cacttgctgt
    17761 tggctgtgga ctgaatgttg cacctgctca ggcgcataaa tttgccagca attaccattg
    17821 ttttatggtt cgtcgttttg accgcactaa tgcgggaagg cgtctgcatt ttgcttcggc
    17881 tatgacacta accaggcatc aggatggtga agatgcctct acaggtgtga gttatctgga
    17941 attagcagat gttcttatca ggcatggcgc gcagactaat actgatctta aggaactctg
    18001 gtccaggatt gtttttaata ttcttgtttc taataccgat gatcatttac gcaatcatgg
    18061 atttatcttg ataccaggaa aaggttggcg gctttctgag gcatatgatt tgaatcctgt
    18121 ggtcaggtct gatggtttaa aactcaatat tacggaaaat gacaatgcac ttgatttgga
    18181 attagctaga gaagttgcag agtattttcg acttagtttg acggaagctg atgacattat
    18241 tgctaatttt agagggattg tcagtcagtg gcgaatcatt gccgagcgtc tgagattacc
    18301 tggtcgtgag caagagttaa tggcagaggc attcagagcg tcaatttaga atagttattt
    18361 cgatatattc gctaaatcat aaatttgcga agcaatgcca gtagatactg aacgttttgg
    18421 aagagacgtt taatttatga gaaaaataga aacgctacaa agctaaatta tagcgtttct
    18481 atgtaaaata attagcttgt cgctaactgt acttgtggtt cttcgtcttt aactttgata
    18541 agcatccaca gcgcaatggc gccaataatg tcaaagaaag ccagggcaat aaagaacggc
    18601 gcgaagccaa taatgtgaac gaatgccccg ataaacaggt tgaaaatcaa ctgaccgctc
    18661 catgcacagg ctcctgccat acccacagcg gtagctactt cgtctttttt gaataggtca
    18721 ccgcccagag ttgctgcaac agtagaaaga cattgatgcg agaatgcgcc aatgctaatc
    18781 agtaatacgg cgatgtaggg attttcgaca atactcacca gaccaatcgt catcataatg
    18841 acggctgcaa tagtgaaggt aattcttcgt gagttaatta aactcacgcc gcgatcgtgg
    18901 aagaacttcg cgacaaaacc actggctaaa cagccgagat cgcccagcag gaaaggtagc
    18961 cagacgaaca tggcaatttc tttcaggcta aaatgcagcg tttcgacgaa gaaaatcggt
    19021 acccagaagt taatggttcc ccatgccggg tcagcgagaa aacgcgcgat gccaatcccc
    19081 cagaaattac gttggctgag gatatgcttg attgacgttt tttctttctt atctgtttta
    19141 agatgctgtt cttgcccaga ctcaatatag tggcgctctt catcagaaag gcgtttagca
    19201 tcttttggat ctttataaca aaagaaccaa aataaagccg cgagtaaagc aaggctacct
    19261 gagacaataa acgcaaattg ccagctatga aacataatgc accaggcaat caacggtgga
    19321 gcaagcatcg cgccgagtga ggtgcccata ttgaaaacac ctgtcgccac gccacgctct
    19381 tttgccggga accatgttga tgcggttttt acacccgcag gaattgccga tgcttcgctg
    19441 aaacccatca gggcgcgcat aaatgccaga cctgaccatg ttcctgtcaa ggcatgcccc
    19501 attgtcgcca ggccccagat tgcggcacat atcgcaaaac ttaacttcag acccacggta
    19561 tcaataaaga agcccattat cggttgcccc aatgtatagg ccaactggaa tgcactgaca
    19621 atccatgaat attgttcttt ggttattccg gttgtctcca taatcgttgg agccgcaata
    19681 cctaacgccg ttctgtccag ataattgatt acagtgataa agcatacgag ggcaatcata
    19741 tgccatcgat aatt
//
LOCUS       DAZKJR010000095        19737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKJR010000095 DAZKJR010000000
VERSION     DAZKJR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.095"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            210..710
                     /gene="tssB"
                     /locus_tag="ACHOWS_003438"
     CDS             210..710
                     /gene="tssB"
                     /locus_tag="ACHOWS_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFQ0048966.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            745..951
                     /locus_tag="ACHOWS_003439"
     CDS             745..951
                     /locus_tag="ACHOWS_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0048967.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPY"
     gene            1020..2495
                     /gene="tssC"
                     /locus_tag="ACHOWS_003440"
     CDS             1020..2495
                     /gene="tssC"
                     /locus_tag="ACHOWS_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFQ0048968.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2502..2915
                     /gene="tssE"
                     /locus_tag="ACHOWS_003441"
     CDS             2502..2915
                     /gene="tssE"
                     /locus_tag="ACHOWS_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFQ0048969.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2919..4769
                     /gene="tssF"
                     /locus_tag="ACHOWS_003442"
     CDS             2919..4769
                     /gene="tssF"
                     /locus_tag="ACHOWS_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFQ0048970.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSHPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4733..5815
                     /gene="tssG"
                     /locus_tag="ACHOWS_003443"
     CDS             4733..5815
                     /gene="tssG"
                     /locus_tag="ACHOWS_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFQ0048971.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5840..7120
                     /gene="tagH"
                     /locus_tag="ACHOWS_003444"
     CDS             5840..7120
                     /gene="tagH"
                     /locus_tag="ACHOWS_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFQ0048972.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCVFTQQGNVGHGPECQWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITAGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7117..7641
                     /gene="tssJ"
                     /locus_tag="ACHOWS_003445"
     CDS             7117..7641
                     /gene="tssJ"
                     /locus_tag="ACHOWS_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFQ0048973.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7644..8975
                     /gene="tssK"
                     /locus_tag="ACHOWS_003446"
     CDS             7644..8975
                     /gene="tssK"
                     /locus_tag="ACHOWS_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFQ0048974.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGILPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWAEIAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8980..9741
                     /gene="icmH"
                     /locus_tag="ACHOWS_003447"
     CDS             8980..9741
                     /gene="icmH"
                     /locus_tag="ACHOWS_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFQ0048975.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FYGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9750..12533
                     /gene="tssH"
                     /locus_tag="ACHOWS_003448"
                     /gene_synonym="clpV"
     CDS             9750..12533
                     /gene="tssH"
                     /locus_tag="ACHOWS_003448"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFQ0048976.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEINLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQALVQEIIALRQQLLGVAEDD
                     AAPLPDADTVEDTPPESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVEDA
                     QNDESVTKDETVL"
     gene            12530..13273
                     /gene="vasI"
                     /locus_tag="ACHOWS_003449"
     CDS             12530..13273
                     /gene="vasI"
                     /locus_tag="ACHOWS_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFQ0048977.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTP
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13278..14690
                     /gene="tssA"
                     /locus_tag="ACHOWS_003450"
     CDS             13278..14690
                     /gene="tssA"
                     /locus_tag="ACHOWS_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFQ0048978.1"
                     /translation="MAMNLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQHDALSGELAKLAQCWQSYN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAT
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14799..18233
                     /gene="tssM"
                     /locus_tag="ACHOWS_003451"
     CDS             14799..18233
                     /gene="tssM"
                     /locus_tag="ACHOWS_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFQ0048979.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGIYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     GLFPQALLAEPNLATESRTWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPLGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKL
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPANSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18244..19654
                     /locus_tag="ACHOWS_003452"
                     /pseudo
     CDS             18244..19654
                     /locus_tag="ACHOWS_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19620..>19737
                     /locus_tag="ACHOWS_003453"
     CDS             19620..>19737
                     /locus_tag="ACHOWS_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFQ0048980.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQI"
BASE COUNT         4603 a         5449 c         5492 g         4193 t
ORIGIN      
        1 tcacacaatc tatttaacgc ttgcagaata cccctcttta cgatcgataa gaagtccttt
       61 tcttatttat tttgaagtgt agaatgcggg gtgatgaagg agtggtaatt ttattttcac
      121 cgccattagg gcggcattgc cgatattttt ttgcggtaca aatatgccgt gttcatggcc
      181 tgtacgagat aacatgacgg atcactttta tgagcaaaaa atttgaaggc agcgtcgcgc
      241 ctcgcgagcg tataaatata tcctatgtcc cgaagacgga cggccagacg gctgaagtag
      301 agcttccgct taacatgctc gttgtcggcg ataccggaaa tacgcaggag acatccccac
      361 tggatgaacg tcaggcagtt tccgttaaca agcacaattt cggtgcagta atggctgaag
      421 cagccattgg cctcaatttt accgtccccg ccacgctgaa gggaagcaca acggacgatg
      481 aactgaatgt ggcgctgaat atcaagtcac tggatgattt ttcaccggac agcgtggccc
      541 ggcaggttcc ggaagtgaat aaattgctgg aactgcgtga agcactgact gcgctgaagg
      601 ggccgatggg caaccttccg gcattccgta cccagctcca ggcattgctg gaaaatgagg
      661 agtcccgcga gcagttgctt aaagaaatcg gccaggtgag tgggaaataa ccaccactat
      721 ttcattaaac agggttatcc agacatggcg gcacacaaaa atatctccgg gaaacacccc
      781 ctctctgacg aagacatttt gcaggcggtt taccgggtat gtttcagccc gtccggtgaa
      841 atcaaccggg atggcgtgga tgcgctgatt acaacactgg atcgccgtat cagtgatcta
      901 ttggatgaga ttatccatct ttgtgagttt cagcagagcg cgccgtactg acggcgcgta
      961 cttcactgac ggcgaaaacc cggtttcaga aacacagcta aaacagaagg attttcagta
     1021 tgtctttaca ggaagaggaa ctcgtttcca gtcatgccgg gcagactgat caggcgtcgt
     1081 cgctgcttga tcaaatcatg gcacagaccc gtattcagcc tggttcggaa ggttatgacg
     1141 ttgcccgcca gggtgtgaca gcctttattg ccagcatcct gcaatccact gcatctgccg
     1201 aaccggtcaa taaactggcc gtggacagca tgattgcaga tatagatgaa cgcatcagcc
     1261 gccagatgga tgtcatcatc catgcgcctg cctttcagca ggtcgagtcg ttctggcgct
     1321 cactgaaaac catggtggat cgcgttgatt tccgtgaaaa catcaaggtc aacgtgcttc
     1381 atgtcacgaa gcaggaactt ctggaagact ttgaatttgc cccagaaatt attcagtccg
     1441 gattttataa acacgtttat tcgtccggat tcggacagtt tggtggtgaa cccatcgctg
     1501 ccgtgctggg cgcttatgag ttcaaaaata ccgctcctga catgaaactg ctgcaatacg
     1561 tcagtgccgt gggcgcaatg gcgcacgcac cgttcctgtc ctccgtttcc ccggagttta
     1621 tggggctgaa ctcgtggacc gaactgccta atatcaaaga tctttatgcc atctttgaag
     1681 gtccggccta caccaaatgg cgcgccctgc gtgactcgga agattcgcgc tatttagggc
     1741 ttacggcacc ccgcttcctg cttcgccagc cctattcacc aacagataac cctgttaaga
     1801 actttaacta ttacgaagat gtcagccaga accacgaaga ttatctgtgg ggtaatacgg
     1861 cctggatgct ggcatgcaat atcgcagaca gttttgccaa ataccgctgg tgtccaaaca
     1921 ttatcggtcc gcaaagcggc ggcgcagtga aagatctgcc ggtgcatctg ttcgaaacga
     1981 tggggcagat tcaggccaaa atcccaaccg aggtgctggt caccgatcgc cgcgaatttg
     2041 aactggctga agagggtttt atcaccctta ccatgcgtaa agactctgat aatgcagcct
     2101 ttttctctgc aaactcggta caaaaaccga agcactttcc gggaaaagac gcagaaacca
     2161 actataagct gggtacgcag cttccttatc tcttcatcat caatcggtta gcgcactaca
     2221 tcaaggtgtt gcagcgtgaa caactggggt catggaaaga aaggagcgac ttagaaagag
     2281 agcttaatac ctggatccgt cagtatgttg ccgaccagga aaacccgcct gcagacgtgc
     2341 gcagccgcaa acctctgcgc gctgccaaag ttgaggttat ggatgtagaa ggcgaacccg
     2401 gctggtacca ggttgcgcta agcgtgaggc ctcattttaa atttatgggg gcaaattttg
     2461 agctttccct ggttggccgg ttagacaggg agtaacacac catgataccg gatcgggaaa
     2521 gaaccacagg ttctcttttt gagcgtatgg aagcctcttc agccagaaac cggcagggag
     2581 gcagtattca ttcgctcagg caatccattc gccagaacct acgaaacatt cttaataccc
     2641 ggtccggtag ttgccggggt gcgcctgagc tgggcattga tgaaccggaa ggggctgaaa
     2701 acttccgcga atcaatgagt cgggccatcg agcaatgtat agaacgctat gaaccccgga
     2761 tctcacacgc tgaagtgcag gcagtggttt catccgcctc cagcccgctg gatatgacgt
     2821 ttcatattac agcctgggtg acatttaacg aaacgcatga ggtgcttgaa tttgatatgg
     2881 caccgaatgg cagccagcat taccgggtgg actgacaaat ggaatttgaa gaacgctatt
     2941 tccgggaaga actcgattac ctgcgccagc ttagcaagct gctggcaacg gaaaaacccc
     3001 atctggcccg cttcctggcc gaaaaagatg cagatccgga tattgaacgc ctgctggaag
     3061 gggtggcttt tcttaccggc aatctccgcc agaaaattga ggatgaattc ccagaactga
     3121 cgcacgggct tattaagatg ctctggccta attacctgcg tccggttccg gcaatgaccc
     3181 ttattgaata tacgccggat atggataagt cttctgtacc ggtgttaatc ccccgtaatg
     3241 agcagtttac aaccaacgcc ggggaaatca gagttgatga agtgctgccc tctgatgcta
     3301 aaaaggagga gcctcctccc tgtaccttca ccctctgccg ggatatctgg ctgctgcccg
     3361 ttcgcctgga gcagattgaa aaccgcagta cgacccgtaa tggtgttatc aacatcacct
     3421 tttcggtcgc accgggaacg gacttccgca cgctggatct gaacaaactt cgcttctggc
     3481 tcggcaatga cgacaactat acccgtgacc agctttattt atggttctgc gaatacttgc
     3541 agggtgctga cctgactgtg ggtgaacagc atcttcgcct gcctgagttt atgctaaaag
     3601 ctgtcggttt tgagccgcag gatgccatgc tgccctggcc gaaaaacgtc cacagcggct
     3661 accggatcct tcaggagtat ttctgttacc ccgatgcgtt tctctttttt gatctttgtg
     3721 gttgtccggc tttgcctgac ggattgcagg cggaattctt taccctgcaa ctgcgttttt
     3781 cgcacccatt gcccgtggac atccggttgc gccgcgactc cctgcgcctg tattgcgcgc
     3841 ctgccattaa tttatttatc caccatgcag aagccatcac gctggacaac cggcgggcag
     3901 actatccgct ggttcccagc cgccattatc cggaacatta cgatgtcttt tccgttaacg
     3961 gcgtgataag ccaggtgcag gatatgttca ggaaaaaaga tctgggtcgt cctgtttcga
     4021 cgcaggccgc gcgccagtgg cctgcctttg aaagtttcag ccatcagatg gaatacagcc
     4081 ggaagcggga agtggtgtac tggcatcacc ggaccaaaac atccctgttc catcgcggct
     4141 ttgatcatac ccttgctttt atacatgctg atggcagtta tccgtcagac gaatcgctgc
     4201 tcagtaatga agtggtttcg gtatcgctga cctgtaccaa ccgtgaactt ccgtcacaaa
     4261 ttcgttccgg cgatatcacc ggcacaaccg gtaaaaatgc ggctgttgct tcatttcgca
     4321 acattacccg cccgacgcaa ccgctctggc cggtcattga tggcagcctg cactggtccc
     4381 tgctctccgc catgaacctg aattatctgt cattactgga tacggacgcg ctgaagcagg
     4441 tcatcgccaa ctttgatcgc cacgcaatcc atcatccgca gacggcgcgg ctgtcacaac
     4501 aaaagctgga tgccattgag cgtctggaga cccgccctgt cgatcgcctg tttacgggca
     4561 ttcccgtccg ggggctggcc tccacgctgt atctgcaccc ggagccgttt gtctgtgaag
     4621 gggaaatgta tctgctcggt acggtgcttt cgcattttct gtcgctgtac gccagcgtta
     4681 actcattcca catgctgacc gttgtgaaca cagaaagcca ggagacatgg aaatggacgg
     4741 aaagaaccgg gcagcatcct cttatctgag tccgggaaat cccccggcag ataaagagca
     4801 gaatgatccg ctggcacagg tttttcataa cgcctgctct tacaattttt ttgcgatggc
     4861 ggagctgttg caccgcctgg caaagggtga aaaggggacg ccagaattat ccctgcgtga
     4921 cgatccggca caggaaaccc tgcgtttcag cgccgatgcc agccttgcct tcccgtgcag
     4981 tgatatcagt gcgctgaaac gggatacgtc aggggcattc aggatgacca ccacctttat
     5041 ggggttacag gggagccagt cgcctctccc cggttattac ctcgatcacc tggcctggaa
     5101 agcggtacat gaacaaagtc cggtaggtga ttttctggat atgttcagcc accgcctgac
     5161 ccagtttgtc tggcatatct ggcgtaagta ccgctatcac atcagttttc gtaacggcgg
     5221 tgtggatgcg ttctcgcagc gcatgtactc ccttgtcggg ctgggccacc gccagcttcg
     5281 cgataagctg gcgattaatc acagcaaaat gctggcgtat tccgggatcc tggcaaatcc
     5341 gggacgatcc ccggaaataa tctgcggcct ggtgtcccac tgctttgatt tgtcagacgt
     5401 gacgctgcaa aactggcaac gtcgcaaggt ggatattgaa ccggatcagc aaaacagtct
     5461 ggggagctat tcacttaaaa atggcgaaaa actggcggga agatcggtac tgggcaactt
     5521 tgttctgggt acacgggtgc ccgatctcag cggaaaattc cagctcagta ttaccagtct
     5581 gacccgaaaa cagttcctct cttttctgcc gtccggcgaa aacttcctgc cactgacgat
     5641 gtttgtgtcc ttcatcctgc gcgatcagct tgcctgggac ttacatctgg ggctggcgcc
     5701 ggagcaggtg ggcgcaatgc gcctgggcga taacaaaagt gcgctgctgg gctggaccag
     5761 cttcctcggc acaccggaag aacgaccctc agtcaccatc agggtacggt cgtaaattat
     5821 gataatcacg gagcagaata tgccggaaga gaaactacag acgctttcat tgcaggtcat
     5881 taacggcagt gagctggaaa gcgggcgggc ggcgcgctgt gtgttcacac agcagggaaa
     5941 tgtcggccat ggccccgaat gccaatggtc ggtacaggat cgtcagcaga gcattccggc
     6001 tcaggctttt accgttatcc tgcacgatgg cacattttgt ctgcgcccgc agacggcaca
     6061 actgtggctg aaccaggcaa aagtcacagc aacatcagac ctgatacagc tgcgccaggg
     6121 cgatgagatc cagatagggc gtctgatggt gagggttcat ctgaaccggg gagatatccc
     6181 ccattacgat gaggaaatgg ccactcccga aaccatcgtc accaatcgcg atatgctcac
     6241 ggataccctg ctgtcaacgg agggtgcgcc acactatccg ggaatgactc accggcatca
     6301 gcttgcagac accgtggtaa acggtttttc taccgatcca ctccaggcac ttcagtccga
     6361 aagcctgatt accgcgggcg atccgctttc aggcattgcg gctgtccggc catcggcacc
     6421 gctgtccgat ccggcaagta atggggggat caatactccg tttatggatc tgccgcccat
     6481 ttatgccagc cccggcgatc gtaatgatga cgtctctgtg gcagaaatgg cgcaacgcca
     6541 ccttgcggtc acccccttac tgcgcggtct cggcggttcg cttaccgtga gcaattccga
     6601 cgatgcggat gattttctgg aagaggccgg acgcacgtta caggccgcaa taaaaggtct
     6661 gctcgatttg cagcaacagc gtaacagcct ctcagacaaa catttgcgcc cgctggaaga
     6721 taacccgctg cgcctgaaca tggattacgc caccgcgctc gacgtgatgt ttgccgaagg
     6781 taaaagtccg gtacatctgg cggctcccgc cgctgtcagt gaaagcctgc gcaatgtccg
     6841 ccaccacgaa gaagctaacc gggcagcgat tgtggagtca cttcgtgtcc tgcttgacgc
     6901 tttctcacca caaaatctgc tgcgccgctt tgtgcagtac cgccgcagcc atgaactgcg
     6961 ccagccgctg gatgatgccg gagcatggca aatgtacagc cattattacg aagaactggc
     7021 ctccgatcgc cagcaggggt ttgagatgct gtttaacgag gtctacgccc aggtctatga
     7081 ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga acaggaaggc gtttctggcc
     7141 tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg cgaaaaaaat cagcgaggtg
     7201 atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc aaccttcgga aataaccgtc
     7261 accctgctga ccgaaccgga caccaatacc aatgcggagg gtgaatcagc cgccgtggat
     7321 gtgcaactgg tgtacctgac tgacgactca aaattgctgg cagccgacta tgaccagata
     7381 gccagtacgc cgctaccgga tgtgttgggt aaaaactata tcgaccacca ggatttcaac
     7441 ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc tggatgagaa aacgcagttt
     7501 attggtgtcg tggcctattt ctccgatgac caggccactg aatggaaaca aatcgagacg
     7561 gtggaaggaa ccggccacca ttaccgcctg ctggtgcatg tacgtcaaag cagcatcgaa
     7621 atgaaaaaag aggatgaata attatggcaa ccacccgcaa caaggtgatg tggcaggaag
     7681 ggatgctgat gcgcccacac catttccagc aacagcagcg ttacaacgac tacctggata
     7741 accagcgttt ccgggccatg aatgatttat cctggggatt taccgaactc accctcaaca
     7801 atgaactgct ggcgcagggt aagatcatga ttgacagcgc gtcaggcata ctgcccgacg
     7861 gcaccgtctt ttctatcccc gaccaggacg cactgcccga tccgctgcac ccgcaaaatt
     7921 ttccggacga aagaagccgc aatatttacc ttgctctgcc ggtcgccagt gatgtgagaa
     7981 atgaaatcag cgacgggcgg cgaatcgggc gttaccggct gaattatgcc gatgtccggg
     8041 atctgcattc agaagaaggt gacgcgcgaa cactgacgct gggtcaactg acgccgcgca
     8101 ttatgagcgg tgcagaagat atgagcgcct atattacgct cccgctttgc cgtatcagcg
     8161 atcgccatgc cgacggctcc ctgacgctgg atgacgattt tatcccctcc tgccagaata
     8221 ttcaggtcag taagaaactg cgtgtttatc tcaaagaggt acaaggggcc attggcggac
     8281 gggcaagcga tctggcaaac cgcattggct ctccggcgca gagcggcatc gcggatgtgg
     8341 cggaatttat gatgttgcag ttacttaacc gtaaccagac ccggtttacc catcgcgctc
     8401 gtcgatccca gctccacccg gaagatttct accttgatct tgccgggttg ctgggtgaac
     8461 tgatgacctt taccgagccg tcgcgcctgc cctgcccgct tgatgtgtat gatcatcatg
     8521 acctgaccaa aacatttaaa acgctgttac cggaagtcaa acgggcgctg cataccgtac
     8581 tgtcgccaag agccgtcaat ctgccgctgc atctgcgtga cggtatctgg caggccgata
     8641 ttcatgacac agaactgctg caatctgcca cctttgtgct ggccgtggcg gcaaacatgc
     8701 cggtcgatca gatccagcgc cagtttatcc agcagtcgaa aatttcctcg ccggaaaaaa
     8761 tccgcaatat ggtcagtgtg cagatacccg gcattccatt gcgtgccctg atggtggctc
     8821 cccgccagct tccttaccat tccgggttca gctatttcga actcgacaaa agcggccagg
     8881 cctgggctga aatagctgcc gccggggccg ttgcactgca tgtttccggc agtttcccgg
     8941 atctgaacat gcaactgtgg gcgataagag ggtaagacga tggatgaggg gtcactgtct
     9001 ctgccaccat ttaccggaca cgacgaaaaa agccagcgcg actaccatct cgcgcttcgt
     9061 ggcaacagcc tgaatccgat gatcgacgcg gccacgccgc tactcggtat ggtaatgcgc
     9121 ctttcaacca tgaacagcca gacgatgccg gagcatcttt ttgctcaggt ggtgacggat
     9181 gttcaggccg tggagcaact gcttcaggag cagggctacg aaccgggcgt gattatctcg
     9241 tttcgctaca tcctctgcac gtttattgat gaagcggcgc tcggtaacgg ctggtcgaac
     9301 aagaacgagt ggatcaaaca gtcattgctg gtccatttcc acaacgaagc ctggggtgga
     9361 gagaaagttt tcatcctgct ggagcgcctg atccgtgaac ccaaacgtta ccaggatctg
     9421 ctggaatttc tctggctctg tttttctctg ggatttcgcg ggcgctacaa agtggcagca
     9481 caggatcagg gggaatttga gcagatctac cgccgcctgt accacgtcct gcataaacta
     9541 cgcggcgatg cccctttccc gctgttacac caggacaaaa aaacccaggg cgggcgttat
     9601 cagctcatca gtcgcctgac cattaaacat attttttatg gtggcgttgt cgtgctggcg
     9661 ttgttttacc tgttctatct gttgcggctg gacagccaga cgcaggacat cctgcaccag
     9721 ctaaacaaat tacttcgata aggacgttta tgatccagat tgatcttccc acgctggtaa
     9781 aacggcttaa cctgttctcc cgccaggcgc tggagatggc cgcctctgaa tgtatgagcc
     9841 agcaggcagc ggaaattacc gtcagccatg tgcttattca gatgctcgcc atgccacgca
     9901 gtgacctgcg ggttattacc cgccagggcg atattggcat ggaagagttg cgccaggcgc
     9961 tgacggtgga gaactacaca actgcccgtt ctgcggacag ctacccggcg ttttccccga
    10021 tgctggttga gtggctgaaa gagggctggc tgctggcgtc ggctgagatg cagcacagcg
    10081 aattgcgtgg cggcgtgtta ctgctggccc tgctgcattc gccgctgcgt tatataccgc
    10141 ctgctgccgc ccggctgttg accggcatta accgtgaccg tctgcaacag gactttgtac
    10201 agtggacaca ggagtcggcg gaatcagtcg tgccggatgc agacggtaaa ggcgcaggca
    10261 cactgacgga cgctgctgac accctgcttg cccgctacgc caaaaacatg accgcagacg
    10321 cccgtaacgg caggcttgac ccggtactgt gccgcgacca cgaaatcaac ctgatgatcg
    10381 acattctctg ccgccgccgc aagaacaacc cggtggtggt gggcgaagca ggcgtgggta
    10441 aaagtgcctt aatcgaaggg ctggcgctgc gcatcgtggc aggccaggtg ccggacaaac
    10501 tgaaaaacac cgatatcatg acccttgacc tgggcgcatt gcaggccggg gcgtcggtga
    10561 agggcgaatt cgaaaaacgc ttcaaggggc tgatggcgga ggtcatttcc tctccggtgc
    10621 cggtcattct gtttattgac gaagcacata ccctgattgg cgcgggcaac cagcagggcg
    10681 ggctggatat ctccaacctg ctcaaaccgg cgctggcgcg cggcgaactg aaaaccatcg
    10741 ccgccaccac ctggagcgaa tacaaaaaat acttcgagaa agatgccgct ctgtcgcgcc
    10801 gcttccagtt agtgaaggtc agcgaaccca acgccgctga agccaccatt attctgcgtg
    10861 gtctgtcggc ggtctatgaa cagtctcacg gcgtgctgat tgatgatgac gccttgcagg
    10921 ccgcagcaac gctgagcgaa cgttatctct ccgggcgtca gttgccggac aaagccattg
    10981 atgtgctgga taccgcctgc gcccgcgtcg ccattaacct ttcctcgccg ccgaagcaaa
    11041 tctcggcgct gaccactctg agccaccagc aggaggcgga aattcgccag cttgagcgcg
    11101 agcttcgcat cgggctgcgt accgacacat cacggatgac cgaggtgctg gtgcagtatg
    11161 atgaaacgct gacggcgctg gatgaactgg aggccgcctg gcatcagcag caggcgctgg
    11221 tacaggagat tatcgcgctg cgccagcaat tgctgggcgt ggcagaggac gatgcggcgc
    11281 cgttgccgga cgcagatacc gtggaggata cgccgccaga gtcagagtca gagtcagagt
    11341 cagagtcaga acaggataat accggtgccg taccggctga tgaggccgac agagaacagc
    11401 cggaagagac cgctgaaaca gtttccccgg tacagcggct ggcccagctc actgccgaac
    11461 tggacgccct gcataacgac cggttgctgg tctccccgca cgtcgataaa aaacagattg
    11521 cggcggtgat tgccgaatgg accggcgtgc cgctcaaccg cctgtcgcag aatgaaatgt
    11581 cggtcatcac cgacctgccg aaatggctgg gcgacaccat caaaggccag gacctggcga
    11641 ttgccagcct gcataagcat ctactgaccg cacgcgcaga cctgcgtcgt ccgggacgcc
    11701 cgctcggtgc gttcctgctg gctggcccca gcggtgtggg taaaaccgaa accgtcctgc
    11761 aactggcaga gctgctctac ggcggtcgcc agtacctgac caccatcaat atgtccgaat
    11821 tccaggagaa acacaccgtc tcgcggctga ttggttcgcc tccgggctac gttggctacg
    11881 gtgaaggcgg cgtactgacc gaagcgattc gccagaaacc ctactcggta gtactgctcg
    11941 atgaagtgga aaaagcgcac ccggatgtgc tcaacctgtt ctaccagtcg ttcgataagg
    12001 gcgaaatggc agacggtgaa ggccgcctga ttgactgcaa aaatatcgtc ttcttcctga
    12061 cgtccaacct cggctaccag gtaatagtcg agcacgccga tgacccggaa accatgcagg
    12121 aagaactgta tccggtgctg gccgacttct tcaaacctgc cctgctggcg cgtatggaag
    12181 tggtgccgta tctgccgctg tcgaaagaga cgctcgccac cattatcgcc ggaaaactgg
    12241 cccgtctgga taacgtgctg cgcagtcgct ttggtgcaga agtggtcatt gaaccggaag
    12301 tgacggacga aatcatgagc cgcgtcaccc gcgcggaaaa cggcgcgagg atgctggaat
    12361 cggtcatcga tggcgacatg ctaccgccgc tctcgctgct gctgttgcag aaaatggcgg
    12421 ctaacacggc gattgcccgg attcggttgt cggcggtgga cgacgcattt acggcagacg
    12481 tggaagatgc tcagaacgac gagtccgtca caaaggatga aacggtttta tgagcaggtt
    12541 gcgcgttttc cggcagacag gatggtttat cgcgggcctg atgacgggct tacccgccac
    12601 tgccgcgccc gcagaaacat cgtcaatggc cggtgtggcg gtcgccgtcg ccaccacgcc
    12661 cccgccggat gcgacagcca cgttacaggc catgcagtcc tgtcggcggg agtccgcggc
    12721 gctggagcgg cttgactgct atgaccgcct gctggcaccg ctgtccccgt cagggtttga
    12781 cggggcgctg gtcaaagccg gttttgtggg cgaggcctgg acccgcgcca cagagcagga
    12841 aaaacgccgc gagggcaaca ccacagaact gctggtgacg caggtgccgg gggagcgccc
    12901 gacggtggtg ataaccaccc cggctatcgg ccatgttccg ccgcgcccgg tgctgatgtt
    12961 cagttgcgtg gacaatatca cccggatgca ggtggcgctg atgcaccctc tggatgtcca
    13021 tgatatcgcc gtcactctga atgccgacag ccgggcattg cgtagccact ggtttgtgcg
    13081 cgagaacggc accctgctgg agtccagccg ggggctgtcc gggattgatg aaattaagca
    13141 actgttcggc gcaaaaacgc tgacagtgga taccggcgca gataacgccg caggaaagct
    13201 gacctttaac attgacgggc tggcaagggc gattgccccc ctgcgcgttg cctgccactg
    13261 ggcgggagaa taaaaatatg gcaatgaact tacgcgatcc gaatgtctgg atatcgcacc
    13321 tgctggaaaa cctgccggaa gaaaaactgg catcggcgct gaaagatgac aacccggact
    13381 gggagtatat cgacggcgaa atcgtcaaac tgggatctct ggcccacagt caactggata
    13441 tcccggaact ccagcgccgg gggctggtca ttctggcttc ggagagtaag gactttcgtc
    13501 tgctggcgca tctgctgaga accctgcaac acgccggtga tcctcatctc gcattgcgcc
    13561 tgctggcgct gtatgtggag cattactggt cggtggctgc gccgcagaat atggcacata
    13621 aaaaacgctt tgccagccag gtcattaaac gctttgaaac aggtattgaa ggcttttcac
    13681 agaacgccgc cacagcgcag cacgacgcgc tgtcagggga actggcgaaa ctggcgcagt
    13741 gctggcagtc atataacgcc ccggaactgg cactggctac cgatgatctt tttgccctgt
    13801 accagcgtgc gtttaatcgt gcggcaccgg ctcctgcccc cactccggcg gcctccgata
    13861 gttcacgaca gaccaccgcc acgtctgaaa gcggcgtgac gcaacccagt gctccggctc
    13921 cccaaatcgc catcgacagt cacgacgaca aagcctggcg cgacacgctg ttaaaagtgg
    13981 cggctatttt atgtgaacgc cagccggact cgccgcaggg ctatcgcctg cgccgccatg
    14041 ccctgtggca atccatcacc agtacacccc aggcggaaag cgatggacgt accccattgg
    14101 ctacggtctc tgccgatatg gtggcggatt accagtcccg gcttgccagc gcggatatgg
    14161 cgctgtggca gcaggttgag aaaagcgtat tgctggctcc ttactggctg gacggtcact
    14221 gtctttctgc acagacggca ctgcgtctgg gttacaaaca ggtggcagac accatccgcg
    14281 atgaggtcat ccgcttcctt gagcgtctgc cccagcttac cgggctgctg tttaatgacc
    14341 gcacaccgtt tctcagtgag cagacgaaac aatggctggc agcttcgccc gacggcaaag
    14401 ttgcaccggt tgcgcaaatc ggtgaggaat cgcaggcagc cagagcctgt tttgctgggc
    14461 agggtctgga ggcggcgctg cgatatctgg acatgctacc cgaaggcgat ccccgcgatc
    14521 agtttcaccg ccagtacctt gccgcacagt tgacggagga ggcggggctg atacagcttg
    14581 cgcagcaaca gtaccggatg ttgttgatga tagggagtca gatgatggtg tctgactggg
    14641 agccatcatt acttacgcag cttgaacaaa aattcacggc agaacaataa aagagaacag
    14701 gagtttttgt gttcagatta ccaacacccc gactactcag cggactcaaa tcagccctac
    14761 gcccggcgat gccccggttt aaagtctccg ctttctggct gctgatactg gcgtggattt
    14821 ttctgctggt atggatctgg tggaaaggcc cgacatggac gctgtatgaa gaacagtggc
    14881 tcaaaccact ggcgaaccgc tggctggcaa cggcggcatg ggggattatc gccctagtgt
    14941 ggcttaccgt ccgggtgatg aagcgcctgc aacagcttga aaaaatgcaa aagcagcagc
    15001 gcgaggaggc cattgatccg ctcagtgtgg agctgaacgc ccagcagcgt taccttgacc
    15061 gctggctgct gcgcctgcaa cgccatctcg acaaccgccg tttcctgtgg cagttgccgt
    15121 ggtatatggt catcggcccg gcgggcagtg gcaaaacgac actgctgcgc gagggttttc
    15181 cgtccgacat tatttatgcc ccggagggcg cacgcggcgc agaacaacgc ctgtacctca
    15241 cgccccatgt cggtaaacag gcggtgatct ttgatatcga cggcacactc tgcgctcccg
    15301 ctgatgcgga tatcctgcat cgccgtctgt gggaacatgc cctcggctgg ctgaaagaaa
    15361 agcgcgcgcg ccagccgctg aacgggatta ttctgacact ggatttaccc gatctgctta
    15421 ccgcagacaa acgccgccgc gagcatctgt tacagacgct gcgcagccgc ttgcaggata
    15481 tacgccagca tcttcactgc cagttaccgg tttacgtggt actcacccgg cttgatttat
    15541 tgcagggttt tgccgccctg ttccagtcac tgaacagaca ggatcgcgat gcaattctag
    15601 gggtcacgtt cacccgccgc gcccatgaaa atgatgactg gcgaacagag ttgaatgctt
    15661 tctggcagac atgggtggat cgaatgaacc tagcgttgcc ggatctgatg gtcgctcaga
    15721 ctcacacccg cgcgtcttta tttagttttt cccgccagat gcagggaagc cgtgaaccgc
    15781 tggtgtcact gcttgagggt ctgcttgacg gcgaaaatat gaacgtgatg ctgcgtggta
    15841 tctatctcac ctcttcgctt caacgcggac agatggatga tattttcacc cagtctgccg
    15901 cccgccagta ccggctgggc aataacccac tggcgtcctg gcccctggtg gacaccgcgc
    15961 cttatttcac ccgtggcctg ttcccgcagg cattactcgc agagcctaat ctggcaacag
    16021 aaagccgcac ctggctgatg cgttcccgtc gccgcctaac ggttttctct accacaggag
    16081 gcgtggcggc actgctgctc atcaccggct ggcatcacta ttacaacggt aactatcagt
    16141 ccggcatcac cgtgcttaag caggcaaaag cctttatgga tgtaccgcct ccgctggggg
    16201 aagatgatta cggcaacctg caactgccgc tgctgaaccc ggtacgcgat gccacgctgg
    16261 cctatggcga ctggggcgac cgcagccgtc tggccgatat gggactgtac cagggacggc
    16321 gtatcgggcc ttatgtggaa caaacctatc tgcaactgct ggagcagcgt tacctgcctt
    16381 cgctgtttaa cgggctggtc aaagcgatga acgccgcgcc gccggagagt gaggaaaaac
    16441 tcgccgtgct gcgtgtgatg cgaatgctgg aggacaaaag cggacgcaat aatgaggtgg
    16501 tgaagcagta tatggcaaaa cgctggagcg aaaaattcca cggccagcgc gatatccagg
    16561 cacaactgat gtcccatctt gactacgcgc tggctcatac tgactggcat gcagagcgtc
    16621 aggcgggcga cggtgacgcc atcagccgct ggacgccata tgacaagctc gtggtatcag
    16681 cacagaaaga actgagcaaa ctgcctgtct accagcgggt ttatcagagc ctgaaaacgc
    16741 gggcgctggg cgttcttcct gctgacctca atctgcgtga ccaggtaggg ccaacctttg
    16801 accaggtgtt tacatctgcc gatgacaaca aactggttgt tccacagttt atcacccgct
    16861 atggcctgca aagctatttt gtaaaacagc gcgatgaact ggttgaactg acagcgatgg
    16921 attcctgggt acttaacctc acccgcagtg tgaaatacag tgacgccgac cgtgcggaaa
    16981 tccagcacca gttgaccgag cagtatatca gcgactacac cgccacctgg cgggccggga
    17041 tggacaatct gaatatccgc aattttgagt ccatcggaca actgaccggg gcgctggagc
    17101 aggttatcag cggcgaccag cctttgcagc gggcgctgac cgtgctgcgt gacaacacac
    17161 agccaggcgt cttttctgaa aaactctctg ccaaagaacg ggaggaagcc ctggcagagc
    17221 cggattacca gttactcacc cgcctcgggc atgaattcgc cccggcaaac agtaccctgg
    17281 cagtacagaa agacaaagaa agcacgatgc aggccgtgta tcagcaactc accgagttgc
    17341 accgctacct gctggcaatc cagaacgcgc ctgtaccagg gaaatcggcg ctgaaagccg
    17401 tgcagttacg gcttgatcag aacagcagcg atccgatatt cgccacccgc cagatggcaa
    17461 aaacgctgcc tgctccgctc aaccgctggg ttggcagact gactgaccag gcctggcatg
    17521 tggtgatggt ggaggctgtt cattatatgg aagtggactg gcgcgacagc gtggtgaaac
    17581 cgtttaacga gcaactggca aataactatc cgtttaatcc gcgttctgca caggatgcct
    17641 cactggatgc cttcgaacgc ttctttaaac cggatggcat actggatacc ttctaccagc
    17701 agaacctgaa gctgtttatc gataatgacc tgagtctgga ggatggcgat aacaacgtca
    17761 ttattcgcga agatattatt gcgcaactgg aaactgcgca gaaaatccgt gacatcttct
    17821 tcagcaaaca gaacggtctg ggaacatcct ttgccgtgga aacggtatcg ctttccggca
    17881 ataaacgccg cagtgtactg aaccttgacg gtcagttagt cgattacagc cagggccgta
    17941 actataccgc ccatctggtc tggcctaaca acatgcgcga aggcaacgaa agtaagctga
    18001 cgctcatcgg caccagcggc aacgcgccgc gcagtatcag cttcagcggg ccgtgggcgc
    18061 agttccgcct gttcggggcc ggacaactga ccggagtaca ggatggcaac tttaccgtgc
    18121 gctttagcgt ggacggtggc gcgatgacct accgtgtgca taccgacacc gaggataacc
    18181 cgttcagcgg tgggttgttc agccagtttg gtctgtcaga cacactgtac tgaggaggca
    18241 gggatgaata gtaacgtact gacacaaact atcgttaccg gcagtgaccc gcgcgggctg
    18301 ccggaattca gcgccattcg cgaggaaata aacaaagcca gccacccgtc acagcctgag
    18361 ctgaactgga aactggtgga gtcgctggcg ctggcgattt ttaaagctaa cggtgtggat
    18421 ttacacaccg ccacctacta tacgcttgca cggacacgga cacagggact ggcgggcttt
    18481 tgcgaaggtg cggaactgct ggcggcaatg gtaagccacg actgggataa gttctggcca
    18541 cagggcggtc cggcgcgtac tgaaatgctg gactggttta actcccgaac cggcaatatt
    18601 ctgcgtcagc aaatctcctt tgcggaatcc gacctaccac tgatctaccg cactgagcgg
    18661 gcattgcagc ttatctgcga caagctccag caggtagagc tgaagcgcgt tccgcgcgtg
    18721 gagaatctac tctattttat gcagaacacg cgtaaacggc ttgaacccca gccgaagagt
    18781 aacgcagaga acgccgcaca gaccacggtc agaacgctga tttatgcccc ggaaacaatg
    18841 gcttcctcca cgccagaagc ggtagtgcct cccctgcccg acctgcctga gatgaaagtg
    18901 gaagtgcgca gtctgtcaga aaatccccca caggccagtg tgatgaagca aggcagtacg
    18961 gtaagagggt ttatcgcagg gatcgtctgt tcagtggcta tcgcctcagc attgtggtgg
    19021 tggcaggtct atccggcgca gcagcaactg ttacaggtta acgacaccgc gcagggcgca
    19081 gcaacggtgt ggatggcctc acctgaactt gaaaactatg agcgcaggct gcatcagctt
    19141 cttggcgtct ccccggtaca accgctggaa accgggatgc agatgatgcg tgttgccgac
    19201 agtcgctggc cggaaagcct gcaacagcag caggcctcga cacaatggaa tgaggcactc
    19261 aaaacccgcg cacagagtag cccacagttg cgcggctggt tgcagacccg ccaggactta
    19321 catgcttttg ctgatctcgt gatgcagcgc gagaaagagg gattaaccct ttcctatatc
    19381 aaaaatgtca tctggcaggc ggagcgggga ctggggcagg aaacacctct ggaatccctg
    19441 ctgacgcagt accaggatgc ccgtgcgcag aagcagaata cagatgcgct ggaaaaacag
    19501 attaacgagc ggatggacgg cgtgttaagt cgctggctgc tgctgaagaa taatacgata
    19561 ccgacgataa aaaaagacat tgaatttcaa taacattcat gaatacaaag gagttttaaa
    19621 tggcgaattt aatttattta acactaaatg gtgataatca agggctcatt tcaagtggtt
    19681 gttcatcaca accatccatt ggaaataaag ctcaaacagc acacattgat caaatta
//
LOCUS       DAZKJR010000096        19521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKJR010000096 DAZKJR010000000
VERSION     DAZKJR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.097"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHOWS_003454"
     CDS             complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHOWS_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0048981.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHOWS_003455"
     CDS             complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHOWS_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0048982.1"
                     /translation="MANWHSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1614..3347
                     /gene="argS"
                     /locus_tag="ACHOWS_003456"
     CDS             1614..3347
                     /gene="argS"
                     /locus_tag="ACHOWS_003456"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0048983.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHOWS_003457"
     CDS             complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHOWS_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFQ0048984.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVSGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3792..5870)
                     /gene="flhA"
                     /locus_tag="ACHOWS_003458"
     CDS             complement(3792..5870)
                     /gene="flhA"
                     /locus_tag="ACHOWS_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFQ0048985.1"
                     /translation="MSNLAAMLRLPANLKSTHWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5863..7011)
                     /gene="flhB"
                     /locus_tag="ACHOWS_003459"
     CDS             complement(5863..7011)
                     /gene="flhB"
                     /locus_tag="ACHOWS_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFQ0048986.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKSILVGSVTG
                     FYLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7200..7844)
                     /gene="cheZ"
                     /locus_tag="ACHOWS_003460"
     CDS             complement(7200..7844)
                     /gene="cheZ"
                     /locus_tag="ACHOWS_003460"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFQ0048987.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7855..8244)
                     /gene="cheY"
                     /locus_tag="ACHOWS_003461"
     CDS             complement(7855..8244)
                     /gene="cheY"
                     /locus_tag="ACHOWS_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFQ0048988.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8259..9308)
                     /gene="cheB"
                     /locus_tag="ACHOWS_003462"
     CDS             complement(8259..9308)
                     /gene="cheB"
                     /locus_tag="ACHOWS_003462"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFQ0048989.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9311..10171)
                     /gene="cheR"
                     /locus_tag="ACHOWS_003463"
     CDS             complement(9311..10171)
                     /gene="cheR"
                     /locus_tag="ACHOWS_003463"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFQ0048990.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNLHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10190..11791)
                     /gene="tap"
                     /locus_tag="ACHOWS_003464"
     CDS             complement(10190..11791)
                     /gene="tap"
                     /locus_tag="ACHOWS_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFQ0048991.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLESASAQSQRNYHISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(11837..13498)
                     /gene="tar"
                     /locus_tag="ACHOWS_003465"
     CDS             complement(11837..13498)
                     /gene="tar"
                     /locus_tag="ACHOWS_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFQ0048992.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     TVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(13643..14146)
                     /gene="cheW"
                     /locus_tag="ACHOWS_003466"
     CDS             complement(13643..14146)
                     /gene="cheW"
                     /locus_tag="ACHOWS_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFQ0048993.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14167..16131)
                     /gene="cheA"
                     /locus_tag="ACHOWS_003467"
     CDS             complement(14167..16131)
                     /gene="cheA"
                     /locus_tag="ACHOWS_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFQ0048994.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRDQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16136..17062)
                     /gene="motB"
                     /locus_tag="ACHOWS_003468"
     CDS             complement(16136..17062)
                     /gene="motB"
                     /locus_tag="ACHOWS_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFQ0048995.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17059..17946)
                     /gene="motA"
                     /locus_tag="ACHOWS_003469"
     CDS             complement(17059..17946)
                     /gene="motA"
                     /locus_tag="ACHOWS_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFQ0048996.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18073..18651)
                     /gene="flhC"
                     /locus_tag="ACHOWS_003470"
     CDS             complement(18073..18651)
                     /gene="flhC"
                     /locus_tag="ACHOWS_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFQ0048997.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18654..19004)
                     /gene="flhD"
                     /locus_tag="ACHOWS_003471"
     CDS             complement(18654..19004)
                     /gene="flhD"
                     /locus_tag="ACHOWS_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFQ0048998.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4637 a         5453 c         4789 g         4642 t
ORIGIN      
        1 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
       61 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      121 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      181 ccttgattac gataacgcat tggtgaggct tgccacgctc ccgcggagct atggacttcc
      241 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccggccata
      301 atgattggag catcaccatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      361 tctgattttt gccctgatgt cagtacccga gcaacgccca attccgcaag attattgagt
      421 gtatttaaag ggttagcgca catatcgaag gcgcgatgaa aagtcacggc cagcggaccg
      481 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaaca
      541 cccgtcacca gtccaggaaa acctaattcg cggactgtgc gcacatcctc aagaatggcg
      601 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaataat aggatgcaca
      661 gggatcgtca cccgctggcg cacggatttc agcaccccca acgacggcgt taagcccccc
      721 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagcgcg
      781 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgccga
      841 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aatttaatgg tcgttttgcc
      901 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gcgaactggt
      961 tttaactgag atccccggca ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1021 attcagcgtt tctggatcgc ccggcagaac gatttcaata tgttcccaac cttcgtgcgg
     1081 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1141 taccggttca tgcaatttaa acagacaaat cggcctgcca ttgatcatat tttctgacaa
     1201 aagctcgccg cactgttcaa acccgcgacg ccagcgctca gcggtggcgt tttgatggca
     1261 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccaaac ggcgggaaag
     1321 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     1381 agaatgccag ttcgccataa tcactcttcg tcttccacta aaagcgttaa tttaccctgt
     1441 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacgaca
     1501 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1561 tctttaactg gtgcgggtaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1621 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1681 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caagctaacg
     1741 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1801 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgcgggt cctggcttta
     1861 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     1921 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     1981 acgtggcgaa agagatgcac gttggtcacc tgcgctctac cattattggt gacgcggcag
     2041 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2101 gcactcagtt cggtatgttg attgcgtggc tggaaaagca gcagcaggaa aacgccggtg
     2161 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2221 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     2281 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcacct
     2341 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggtgaaagc ctctacaacc
     2401 caatgctgcc gggtatcgtg gcggacctga aagccaaagg tctggcagta gaaagtgaag
     2461 gcgcgaccgt cgtattcctt gatgagttca aaaacaagga aggcgaaccg atgggcgtga
     2521 tcattcagaa gaaagatggt ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2581 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2641 aacacctgat gcaggcgtgg gctatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     2701 cgctggaaca ccacatgttc ggcatgatgc tgggcaaaga cggcaaaccg ttcaaaaccc
     2761 gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa cgtgcacgcc
     2821 gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa ctggctaacg
     2881 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     2941 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3001 catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag caactggctg
     3061 cagctccggt tatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3121 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
     3181 tgtacgatct ggccggtctg ttctctggtt tctacgagca ctgcccgatc ctcagcgcag
     3241 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacggcg aagacgctga
     3301 aactgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg cgttatacgg
     3361 caaaagccga tgtcatggca gacatcggct tttttatcat taacgataat tcacaatcac
     3421 ttcattccgt tgtaccttta gcggcggaat taaccgccca ccgccagaca cttcccagat
     3481 aaatcgcaac ggttctatcg ccggtatacc ggaaaaggcc acggtggttc cactctgccc
     3541 ttctaattcg acacaacgag attgtgaaca caagcgaacc cgcagtcctg caggtgtcgg
     3601 gccgataagc tgataacgcc acgccaccag cgtcatcaat cctgcagcag gttgtcgagt
     3661 agaaagaggc gctgacgaca tcgactcacc acggtgattc agcgtaacgc ccacactact
     3721 cgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa
     3781 taaagttctc atcatttacc gccaattgtc gccgtcatgc ggatatgtcg gttatcagat
     3841 agttccagat tcgacagcac cactaactgc ggcaggctgc ggcgcaggaa gcgagacaat
     3901 aatggtcgca gcgcgtggtt caccaacaat accggcggcg cacccaacat ctcctgacgg
     3961 gatagcgctt cctgagtttg cgccagtaaa cgatccgcca gccctggctc cagtcccccc
     4021 ccgccctgca acgcctgtag caacaaacgt tccagcggtg tatcgaggcc aataacatgg
     4081 acttcatctt tgccaggaaa ccactgctgg gtaatcgccc ggcccaacgc tacgcgcacg
     4141 acggcggtta attcatgcgg atcgctttgg atgggcgcat gttccgccag cgtttcgaga
     4201 atggtgcgca tatcgcgaat cggcactttt tcatcgagca gattttgcag cactttatgc
     4261 agtgtggtaa gcgtgacgac accaggaacg agatcttccg tcagctttgg catctcctgg
     4321 gcgacgcgat ccagcagctg ttgtgcctcc tgacgaccaa acagctctgc ggcatgctgg
     4381 ctaatgaggt ggttaagatg ggttgccacc accgtgctgg cctcaaccac ggtgtacccc
     4441 tgaatctgcg cctgttcttt aagcgcactt tcaatccaga tagcattcag gccaaatgcc
     4501 ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg taccagggtt aatcgccaac
     4561 caacgccccg gataagcatc accactgcct atctccacgc ctttcatcaa aatgcgatag
     4621 cgggcaggtt gcagatccat attgtcgcga atgtgcacca ccggcggcag aaaccccatc
     4681 tcctgggcaa atttcttgcg aatactgcgt atacggccca gcaactcacc atcctgctgg
     4741 aaatcgacca tcgggatcag gcgataaccc acttccattc ccagagaatc ttccagttgc
     4801 acatcgttcc acgtcgcttc gacaacggta ttattctctg ccatttttac cggttttggt
     4861 tcggcaggcg ctttttgttc acgtccgcgg atccaccagg ccagtcccag caatccggca
     4921 gtgaacagca aaaataccag gttcggcatt ccaggcacca ggccgagtaa accgagcacg
     4981 gcggcgctta acaacataac gcttgggtta ctgaaaagct gattcaccat ctgctcgcca
     5041 acatcctgat cggtgctgac acgcgtaacg atgacccccg cggcggtaga aatcaccaac
     5101 gccggaattt gtgccaccag accgtcgcca atagtcaata gcgtataact ttccgccgcg
     5161 tgtcccatgc tcataccatg ttgcagcacg ccgaccagca acccaccgac aacgtttatg
     5221 accatgatga ggatcccggc gatggcatcg ccgcgaacaa acttacttgc cccgtccatc
     5281 gagccgtaaa aatcggcttc ctgagtcact tcggagcggc gttttttcgc ttcatcttca
     5341 ccaatcaatc cggcgttaag gtcggcgtca atcgccatct gcttccccgg cataccatcg
     5401 agaacaaagc gcgcgcctac ttccgcaata cgcccggcac ctttggtaat gaccataaag
     5461 ttgatgatca cgagaatgac gaacaccacg ataccgatag cgaaattgcc accaacgagg
     5521 aagtgaccga acgcttcgac cacctttcca gccgccgccg cgccggtatg cccttccatc
     5581 aaaatgatac gggttgaagc cacattaagt gccagacgca acaacgtggt aaacaacaga
     5641 atcgtcggaa acgcagcaaa ctcaagcgtg cgttgggtaa acatcgccac cagcaacacc
     5701 atgatcgaca aggcaatatt gaaggtaaac agcaggtcga gtatgaatgc gggcagtggc
     5761 agtaccatca tcgacaagat caacaggatc aaaatcggtc cggcaaggat ctgccagtgt
     5821 gtcgatttca ggtttgcggg caggcgcagc atcgcggcca gattactcat gggtcggttt
     5881 ctcgttaata aaatccaggg cttccggcac cggaagatga gtaggttgta cagggcgctg
     5941 tccaccagcc agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc
     6001 ggcgtacagt tgacccggga tttgttgacc gatctccgca tgtcgataca gcgcacgcgc
     6061 cagcggcggc gcttcaagcg tcgggacgtt attttcagcg ccaatttcac gaatgcgcag
     6121 cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg ctcattttgt tttcgtcata
     6181 ctgcaaagcc actgagtagt gagttgggtt attgacgata acgtccgctt tcggcacatc
     6241 ggccatcatc cgccgccgcg ccgcagctcg ctgcatctga cggattcgcc ctttgacatg
     6301 agggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgtgaca tacgcagctt
     6361 tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accatcggaa tgacaccaag
     6421 caccaccagc aatgcgcaca gccctaccag atccatcgca ttacccatgg cggtaatggg
     6481 ggactccgcc attaagcgca tcatttgcgg ccagtggtgc cagagataaa accccgtcac
     6541 actgccgacc aggatggatt tcaaaatcgc tttaagcaac tccgcaccgg tttgtgccga
     6601 aaacatccgt ttaatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt
     6661 gccgctaaat accaggccac ccaacataac cggagaaata agcgccacca gcaccacacc
     6721 gctaatcagc ggcagcagcg ccagcatggc ttctctgatc agcagaataa tttgcccgag
     6781 gatcagattc gggtcattga taatactgtg atcaaaatgc agcccagcgg agagcatgcc
     6841 cgacaatcga cgggccagcg acacaccgcc aaaccagata acacacacgc ccactaacaa
     6901 aatcagcagt gacgttaatt cacgtgaacg cgggatttgc ccctcttccc gcgctttttc
     6961 tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat
     7021 cctggaaaga gtattaagcg taaatgatgc cagagcgcaa agcgttcaat ggtttgagta
     7081 aggggcaaaa caggcgggat ttagggcttt tactgccaca catcaagcat agtatgcgtt
     7141 tgtcggatgc ggcgtcatcg cctgatatga cgtggtcacg ccacatcagg caatacaaat
     7201 caaaatccaa gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct
     7261 ttgctggtat cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac
     7321 tcctgttccg ggatgttttc caacagcacc atcaacaact ggcgttcgat ctcctgaatg
     7381 acatccatca tccgcttaat aacctgcccg gtgagatcct gaaaatcctg cgccatcatg
     7441 atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg ggacatctgc cagaaattgc
     7501 cgcgtatctg ttaccagttc tcgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca
     7561 tcccaacgtt gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac
     7621 gcctcaacac tgttcagcgc ccgctccgca gcctgggcag tcatctgcac aacatagtac
     7681 aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc
     7741 agttcccgca aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg
     7801 ccagctgaat gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat
     7861 gcccagtttc tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt
     7921 caccacatag ccactggccc ccgcttgcgc cgcagcaata atgttctctt tcttcgcttc
     7981 tgcagtcacc attaacactg gcaatgctga catcgcgcca tccgcacgaa tcgttttcag
     8041 caattccagg ccatccatgt tgggcatgtt ccagtcggag ataacaaatc cataaccgcc
     8101 tgcctgcaac ttattgagag cgtcgacacc atcttccgct tcctcaacat tattgaatcc
     8161 tagctctttc agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa
     8221 aaatttaagt tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg
     8281 gcactaattt ttgccagcat ttgctggctt acctggctaa gatcgaccac ttcgcaaaca
     8341 ccacccatat tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgt
     8401 gcaagggtcc atgcccccgc ctgacgcatc gccaacattc ccaccgcgcc gtcgttgccc
     8461 ataccggtca ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac
     8521 aacacatcta ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttgg
     8581 taatttgcgc cactacgcgc cagctccata tgccgatcgc ccggcgcaat ataagcatgc
     8641 cccggcaaga cacgttctcc gtcttcggct tctttaaccc cgatctggca aagcttatta
     8701 agtctgtcgg caaaagagcg ggtgaaaccg ggcggcatat gctgggtaat taacagtgcc
     8761 gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa
     8821 gcaccaatcg caatcagttt ttcagagctc aacaacggcc ctgccttcag cgttgtcggt
     8881 gccgacaatg gcttatgtgc tgcaaggctc gcctttgctg ctgtacgcac cttttcagca
     8941 atcatttcgc tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa
     9001 tctatcgccc ccagctccaa tgcgcgcagc gtgacttctg atcctttgcc ggtcagggaa
     9061 gaaaccatca caacgggcat tgggcgcaaa cgcattaatt tttcgagaaa atccagtccg
     9121 tccatccgcg gcatttcaac atccagcgtt agcacatccg gattgaattt cttaatcaaa
     9181 tcacgcgcaa ccagcggatc aggggcggtc gccaccattt ccatgtcgct atggctgttg
     9241 atgatttctg tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt
     9301 ttgctcatcg ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc
     9361 gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga
     9421 gcggaacaaa gcggcgcaaa atctcctgct gggtagtttg atcgaagtag atcatgacgt
     9481 tacgacagaa gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtaaattca
     9541 gcggggcaaa atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg
     9601 gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt
     9661 catggcgata aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcgctggcaa
     9721 acactttcca gcgtccgggc acggtgccca atgtgtcagc cagcgtcatc gcaatgctgt
     9781 acggctcttc gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac
     9841 gtgcgtgatc cgcgagcaga ggaaaatgat gcgcctcacg gaaaaatgcc gtcagattcg
     9901 tggtcagcga attgataaac gcctgccact caccgctgtg cagattagat tccagcaagt
     9961 tcaggtaatg accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt
    10021 aaaccatgtc gcgtttatgg tcggccagga cgatcccggc tcgttgatag atcaattgac
    10081 ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag
    10141 acgtttgccc acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca
    10201 ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac ttcatgctct tcaagggtaa
    10261 ataccgccac gcgcgacgaa agatggtcgg cctgattcgc cagttgttct gttgccaccg
    10321 ccgcttcttc taccagggag gcgttctgct gtgtcacctg atccatctgg ctgacagcct
    10381 gtgcaacctg ctcaatcccc cgccgttgtt cttccgaggc cgaagcaatt tctcccataa
    10441 tgtcgttcac gcgggtcacc gaactgacaa tatcagtcat ggtcgcggcg gcgttattca
    10501 ccagtttcga accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt
    10561 ttgctgcctg ggcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac
    10621 cacgcccctg ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg
    10681 tctggaaagc aatgccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtag
    10741 cgatctcctg catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg
    10801 tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg
    10861 tggcggttaa ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg
    10921 tgcgacttga gagatcgtta ttgcctgcga caatctccgc gataccaatg tgcatctcct
    10981 ggcttccctt acgcacatca cttaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc
    11041 tggcaaaaat ggcggtgatc tcattgcgac catacaccgc aatcggacgt gccagattac
    11101 ccgcagcaat gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc
    11161 gcgtccacca cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca
    11221 ccagcgccga aatgtgatag ttacgctggc tttgcgcact ggcactttcc agcacatggt
    11281 tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat tcctgcaccg
    11341 gcgcagtgag gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat
    11401 cgttgtgcca gcgggcaaaa cttttctccg tctctttttg caacgccctg acgtgctcgt
    11461 tgcccgcagt catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc
    11521 gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata gctaagcgcc gtcagagtgc
    11581 ccgctttgtt cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg
    11641 cacgttgctg attgctctgc tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc
    11701 aaaaagacat gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca
    11761 gcgtggtcga aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg
    11821 cgttagtaaa tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc
    11881 gtggctgtgc cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga
    11941 gtgggctggc tgccagacgg aacgcggaaa ccgtttgcgt taaacgactc gcctgttctt
    12001 ccagcgcagc tgcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc
    12061 gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg
    12121 ccgatgcaat ctcgcccata atgtcagtca cgcgggtgac agcattgacg atattgttca
    12181 ttgtttcccc ggcgctttcg accagcaccg aaccggtatc aacgcgcgag acggagtctt
    12241 caatgagggc tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt
    12301 cgcccgccac cacggcaaaa ccacggccct gttcacccgc tcgcgcggct tcaacagcgg
    12361 cattcagcgc gagtatatta gtctggaagg caataccatc gataacgctg ataatgtcgg
    12421 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
    12481 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
    12541 tatcggcgtt ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca
    12601 gcgcggatgc ctgctgttcc gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
    12661 gggtaccggc atagatggca tctgaacctt cacgcacatg agtgacggtg tcagtcaaag
    12721 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
    12781 tcagggtatt cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca
    12841 gcggtgtaag caacatacgg cgaatgccgt accacgccac cagcagaatc aataccacca
    12901 ccagcgcgat aaccgccagt tgccactggg caaatcggta atcatctgcg ttgtcagtga
    12961 cgatatcgcg atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg
    13021 cattttgcat tccctgggtt ggctgagcga aataagctcc agtattgcca taatcaagat
    13081 aatcaatcag ttcagttaac gctgtgtgat agtttttata tttttcatca atattacgac
    13141 tggtagcgac catttcaggt aacggtgcca tgcttttgaa ttttttataa tgagtcgctg
    13201 cctgggccaa tgttttcctg gcgctatcga gcaattcaac tttggcatta ctttgctgat
    13261 tggatgaatc catcatcatc cgcaccgctg aacgactcag gttaatgcgc gtttgcagca
    13321 ttaaatccca tgttgacgtc agctcgccct gctgttcccg taattgattg gaaaccacaa
    13381 agctcttctg gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca
    13441 gtgcgaatac ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa
    13501 ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag gaagggggaa actttattgc
    13561 tgatgccacc cgccgcgaaa ctgaaataaa aaacccgatg cgcagatcat cgggttcatt
    13621 gcaattgagg aaatcgggag aattacgcca cttctgacgc cgcgctatct aatagcgcca
    13681 tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatacgg tcgcccagtg
    13741 cacccagtcc agtgagatat tctgttgaaa gcgtcacggc aaattccggt gccggacgaa
    13801 tttgctccgc cgtcaatgaa agcacgtctg agacgccgtc aaccacgatg ccgaccaccc
    13861 gctgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc
    13921 tgaacttaat tcgtaagtcc acaatcggca caataacacc gcgcagattc gtgacgcctt
    13981 tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg atctcctgca
    14041 ctttcagaat atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc
    14101 ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt
    14161 actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga
    14221 gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg
    14281 gactttgcga tagttacttt caaggttttt aaccacaacc tggtgttgac caattaattg
    14341 atccaccagc aaggcgtagc ggcgaccgcc actttgtaag atcaccacaa ttccctgggt
    14401 agcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga tgggcagata
    14461 ttcaccccgc acttccagca cccgctcgcc gccagccagt ggatgcagat cggcttcacg
    14521 gggttgcagt gattccatga cagcattcag cggcagaatg aaaacttcat ccgcaacgcg
    14581 tacggacatg ccgtcgagga tggccagcgt cagcggcagt aaaatgcgga tcgtcgtgcc
    14641 agtaccctgc ttcgactgga tttcgacatg accgcccatc tcctggatat tacgtttaac
    14701 gacgtccatg ccgacgccgc gcccggagac gtcggtgacc tgctccgccg tggagaagcc
    14761 aggggcaaat atcagcatcg cgacttcgtc gtcgctcatg ttttcgctga cggtcagacc
    14821 ttgcgaggcc gcttttgcca gaattcgctc acggtttagc cccgcaccat cgtcggtcac
    14881 ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga attaaatttc cgacgctgtt
    14941 tttacctgcg gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac
    15001 caggtgggtc agcgggtcga taatgcgttc tatcaggctc ttgtcgagtt cagtggaact
    15061 gcccaccagc gtcagttcta cctgcttgcc gagttttccc gcaagatcac gcaccagccg
    15121 gggatagcga ctaaagacat actccatcgg catcatgcga atcgacatca ctgattcctg
    15181 caaatcacgg gcgttacgtt gtaactgccc catgctggta atcaaatcac catgattaac
    15241 cgggtccagt tcactagaac gctgggcaag cattgactgg gtgatgacca gctcgccgac
    15301 gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg attcactgct
    15361 gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct tgttcggctg ccagtttaag
    15421 cactggtggg ggggatattt tgggcgagac ttctactgtt tcaaaggtaa tctgatcggc
    15481 ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat
    15541 tgccgagaga gaatccgccc ctttcaccac atcagttaac gttgtcagat gccccagctc
    15601 ttcttccagc aggtcgactt ccccggcctt caggcgcgaa aggataattc gtcgcggcga
    15661 ctgatcgcgg ctctgctcat cttgcggttc acttttggca accacactta atcgggtcac
    15721 tgcagatggc gtttcgcctt tcgcttctaa tgccagttga cgcaaggcct ggcagatata
    15781 atcgaagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat
    15841 gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg
    15901 tctggcttcg tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc
    15961 aaaggttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc
    16021 ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatgtcag ccaacagttc
    16081 gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc
    16141 tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca
    16201 gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt
    16261 gtttgttcag taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc
    16321 ttaagcgcat cgttgccgcc atgccgacga cacgtaacac tttgccgcca ttcaacccac
    16381 caaccatcag ttcgcggcgg gatgcattgg cacgatcggc agaaagctcc cagttgctgt
    16441 aacctttctc accgctggcg tagggaaaat catcggtatg acccgagagg ctaattcggt
    16501 tgggaatacc gttcagtacg ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga
    16561 cgtcggcact gccagttcta aacatcgggc gattctggct atcgatgatc tgaatacgta
    16621 gaccttcctg gaccaaatcg attttgagat gtggacgtaa cgcccgcagt ttcggatcgg
    16681 actcaatcaa ctggtcgaga tcgccacgca atttccgcaa tcgactttgc tccatgcgtt
    16741 ttttcagctc ttcgatgttc ggctgcttat tcacttcccc ctggctttgg gtgtaatcat
    16801 cacccccacc gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgcag
    16861 tcgccagtgg agtccggaag tactccgcaa tctgaatcag ctcttttggg ctggagatgg
    16921 agattaacca catcaccaga aaaaaggcca tcatcgcggt cataaagtcg gcataagcaa
    16981 tcttccacga tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg acgacaataa
    17041 tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac
    17101 cgcacgcaca tgctcttcca gttcaataaa cgacggacgt tcgctggaat agagcgtttt
    17161 gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agattagata gcagtgtgac
    17221 tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cactcgctaa
    17281 tggggaaata aatccgtaag ccaataaaat accgaggaaa gtccccacca tcgcatgtgc
    17341 gataagcgcc cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaacgacccc
    17401 cattacagcc gcaacaatac caaacgccgg aagtgagtca ccgaccagcg ccagactgtt
    17461 cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca gagcttcgat
    17521 ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga taaattcaag
    17581 catgacgcta tccgcaagga tgcgtgggta gctggcgaag atctcgctct cacggggatt
    17641 ttcaatatca cgttccagcg aaaacatccc catctgccgc gatttcgcca tcaaccgata
    17701 aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa
    17761 cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc
    17821 aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc
    17881 accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat
    17941 aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa
    18001 cacgcgatag gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgtttcgcc
    18061 acaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
    18121 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggct gacataagct
    18181 gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt
    18241 gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgggt
    18301 taatgccagc agcggtccct cttctgcttg cgggcactgt tcaaggtata aacggtaggc
    18361 tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcgtt
    18421 acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg
    18481 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta tagagtttta tcaggcgtcc
    18541 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc
    18601 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
    18661 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
    18721 atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
    18781 atcgtctggt gactgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta
    18841 accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga
    18901 cgaaacatag cggacgcttt gtcctgaaca atcagacgct gtgcaagtag taaatatgac
    18961 aagttgatgt cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat
    19021 aaccaacttt atttttatgc ggtctcaccg caccccgtga tgtcgccggg aagccccggt
    19081 aaaaaataat tagcattaga atagttgcga taagctgcaa taggcagagc ccctttgtgg
    19141 gatttaataa gtccttacaa atagaagggg ttctttacac ttatctaaga tttttcctaa
    19201 atcgacgcaa ctttactcgt cactacacgc acatacaacg gaggggggct gcgattttca
    19261 ataatgtgtg atgcagatca cacaaaacgc tcaattactt aacataaatg tataagtcat
    19321 acttttgttt tgggtgtatt tcaatctgtt aaaaagtttt tcgcgacgct agcaagcaaa
    19381 aatgaaacag gaataataga aatgggatgt tgcgcacagt taaaataact caccgtaaat
    19441 aatcatctgc tataaataat tgctttaatg caataccaga taagctattt ttaaacagac
    19501 acttaccgca caacaaacta a
//
LOCUS       DAZKJR010000097        19442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKJR010000097 DAZKJR010000000
VERSION     DAZKJR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.098"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..218
                     /locus_tag="ACHOWS_003472"
     CDS             <1..218
                     /locus_tag="ACHOWS_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004988563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0048999.1"
                     /translation="KNNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQ
                     ALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(202..840)
                     /gene="ytfB"
                     /locus_tag="ACHOWS_003473"
     CDS             complement(202..840)
                     /gene="ytfB"
                     /locus_tag="ACHOWS_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFQ0049000.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1058..1678
                     /gene="fklB"
                     /locus_tag="ACHOWS_003474"
     CDS             1058..1678
                     /gene="fklB"
                     /locus_tag="ACHOWS_003474"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0049001.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1987..3399
                     /gene="cycA"
                     /locus_tag="ACHOWS_003475"
                     /gene_synonym="dagA"
     CDS             1987..3399
                     /gene="cycA"
                     /locus_tag="ACHOWS_003475"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFQ0049002.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3444..4106)
                     /gene="ytfE"
                     /locus_tag="ACHOWS_003476"
     CDS             complement(3444..4106)
                     /gene="ytfE"
                     /locus_tag="ACHOWS_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFQ0049003.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(4214..5179)
                     /gene="ytfF"
                     /locus_tag="ACHOWS_003477"
     CDS             complement(4214..5179)
                     /gene="ytfF"
                     /locus_tag="ACHOWS_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFQ0049004.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPGFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(5287..6147)
                     /gene="qorB"
                     /locus_tag="ACHOWS_003478"
     CDS             complement(5287..6147)
                     /gene="qorB"
                     /locus_tag="ACHOWS_003478"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFQ0049005.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6236..6616
                     /gene="ytfH"
                     /locus_tag="ACHOWS_003479"
     CDS             6236..6616
                     /gene="ytfH"
                     /locus_tag="ACHOWS_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0049006.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6745..8688)
                     /gene="cpdB"
                     /locus_tag="ACHOWS_003480"
     CDS             complement(6745..8688)
                     /gene="cpdB"
                     /locus_tag="ACHOWS_003480"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFQ0049007.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAVFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8878..9618
                     /gene="cysQ"
                     /locus_tag="ACHOWS_003481"
     CDS             8878..9618
                     /gene="cysQ"
                     /locus_tag="ACHOWS_003481"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFQ0049008.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAVAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(9608..10165)
                     /gene="ytfJ"
                     /locus_tag="ACHOWS_003482"
     CDS             complement(9608..10165)
                     /gene="ytfJ"
                     /locus_tag="ACHOWS_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFQ0049009.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            10490..10696
                     /gene="ytfK"
                     /locus_tag="ACHOWS_003483"
     CDS             10490..10696
                     /gene="ytfK"
                     /locus_tag="ACHOWS_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFQ0049010.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(10758..12101)
                     /gene="paeA"
                     /locus_tag="ACHOWS_003484"
     CDS             complement(10758..12101)
                     /gene="paeA"
                     /locus_tag="ACHOWS_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0049011.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(12424..13062)
                     /gene="msrA"
                     /locus_tag="ACHOWS_003485"
     CDS             complement(12424..13062)
                     /gene="msrA"
                     /locus_tag="ACHOWS_003485"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0049012.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            13268..15001
                     /gene="tamA"
                     /locus_tag="ACHOWS_003486"
     CDS             13268..15001
                     /gene="tamA"
                     /locus_tag="ACHOWS_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFQ0049013.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            14998..18777
                     /gene="tamB"
                     /locus_tag="ACHOWS_003487"
     CDS             14998..18777
                     /gene="tamB"
                     /locus_tag="ACHOWS_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFQ0049014.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSMPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            18780..19121
                     /gene="ytfP"
                     /locus_tag="ACHOWS_003488"
     CDS             18780..19121
                     /gene="ytfP"
                     /locus_tag="ACHOWS_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFQ0049015.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
BASE COUNT         4662 a         4859 c         5238 g         4683 t
ORIGIN      
        1 agaaaaacaa tatttttaag ccagatatcg acccattgca ggtgaatatt aatattgctg
       61 cgcttggggg atattatttg atcaaccagc atacgcttgg tctggtttat cacatcagta
      121 tggtttcacc ccaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct
      181 ggcttttggt tgacccttca tctaccgcgc gcgaataaaa ctgccatccg gctggcgggt
      241 aaacaggacc tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc
      301 gttctggcgg attttcacca tctgcccgtt ttgcaaatta ctcagtggct tatccgcgcc
      361 ttctacctgc gccatggcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg
      421 cgccatcgtt ttaccgggtt cgacacgata ggagcgccat tgattatcaa ttccgctatc
      481 tggctggaat ggttgcgtat gggtggtttg cggctgctct tctggctggc cttcctgtat
      541 tggttctgga gcgaccggag ccacctgatc aggatcattt tgtggcgtga ctaattgcgc
      601 ccgcagctgt tcttctgtcg gtggttgtga ttgcgactga atatctaact gcgcttcacg
      661 cgtcaccacc ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ctaccaatac
      721 aatggcggca ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa
      781 attgtccggc gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat
      841 ggtttcctcc tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat
      901 gatgtccgtg gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt
      961 tccccgggat ttgtcatcat tcccgcgaca aagttttacc caaagaagtg tggctgatat
     1021 gctgcccgct actttatacc ctaagaaagg aaatacgatg accaccccaa cttttgacac
     1081 catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac tgagtgaatc
     1141 tggcctggaa gggctgctgc cagaagcact ggttgcaggc attgccgatg cgctggaagg
     1201 caaacatccg gctgttccgg ttgatgtggt gcaccgcgcg ctgcgtgaaa tccacgaacg
     1261 cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg tgaaatacct
     1321 ggaagaaaac gccaaaaaag aaggtgtaaa tagcaccgaa tctggcctgc aattccgcgt
     1381 gatcaaccag ggcgaaggcg caattccggc acgtaccgac cgcgttcgtg ttcattacac
     1441 cggaaaactg atcgacggca ccgtgtttga cagctccgtt gctcgcggcg agccagctga
     1501 attcccggtt aacggcgtga tccctggctg gattgaagca ctgactctga tgccggtagg
     1561 ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgtg gcgcaggcgc
     1621 gtccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa tcctctaagc
     1681 agcgcattct gttcccctcg aaccagaggg gagcagacat tcagcaataa acccttcagt
     1741 ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt ttgtgagctg
     1801 tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca ttagatctaa
     1861 atcttagtat tcataccgcg tattgttacc taatatcgat gagtcccgat acagattcgt
     1921 cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt acaggaagaa
     1981 aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc tgaacagtcg
     2041 ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg tgccattggt
     2101 acagggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc gatcattttc
     2161 gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg ggaattgctg
     2221 ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact cgggccgtgg
     2281 gcaggatatt tcaccggctg gacctactgg ttctgctggg ttgtaaccgg tatggcagac
     2341 gtggtggcga tcacggctta tgctcagttc tggttccccg atctctccga ctgggtcgcc
     2401 tcgctggcgg tgatagtgct gctgctgacg cttaacctcg ccaccgtgaa aatgttcggt
     2461 gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct gatagtcgtc
     2521 ggcctggtca tggtggcgat gcactttcag tcaccgaccg gtgtggaagc gtcattcgcg
     2581 catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt tgccggattc
     2641 cagatagcgg ttttcgcttt cgtggggatt gagctggtag gtacaacagc tgcggaaacc
     2701 aaagatccag agaaatcact gccacgcgcg attaactcca ttccgatccg tatcattatg
     2761 ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc ggtagtcccg
     2821 gagaaaagcc cgttcgttga actgtttgtg ttggtaggtt tgcctgcggc tgccagcgtg
     2881 atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggtgt cttctctacc
     2941 agccgtatgc tgtttggtct ggcccaggaa ggtgtggcac cgaaagcgtt cgctaaactc
     3001 tctaagcgcg cagtacccgc gaaagggctg accttctctt gtatctgtct gctcggcggc
     3061 gtggtgatgt tgtatgtgaa tcccagcgtg attggcgcgt tcacgatgat tacaaccgtt
     3121 tccgcgattc tgtttatgtt tgtctggacg attatccttt gctcgtacct ggtgtatcgc
     3181 aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccactcgg caagctgatg
     3241 tgctgggtat gtatggcgtt ctttgtgttt gttctggtgt tgttgacact ggaagatgac
     3301 acccgccagg cgctgctggt taccccgctg tggtttatcg cgctggggct gggctggctg
     3361 tttattggta agaagcgggc tgctgaactg cggaaataat cgcattatca tgctggatgg
     3421 cgcaatgcca tccagctttt agatcactca cccgccagcg cgcgcgggaa tagtacattg
     3481 ttttccagac tgatgtgctc catcaggtca tcaatcagtt cattaatgcc gttatacatc
     3541 gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt gtgtttgatc
     3601 acttccagca gttcgcccgc ttcatcgtgc tcgctttcca ttacgctgat tggccccatt
     3661 gcctggctgc ccatgccttg tttaatcatc gggaagagaa tttgctcttc tttcatcatg
     3721 tggctggaaa gctcttcatg cagcatagtc aggtattttg tcagcccttt tggcacactt
     3781 ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc cggcagttgc
     3841 tcgcggtggc gatcgtggta gcgcacgatg atatggtcga tgatttctgc cagcggggcg
     3901 ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcggc ttcaatgacg
     3961 tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tctgcttacc gccacagcag
     4021 taatccatat catatttacg aaacagagcc gaagcgcgag gaatagagag cgccagttca
     4081 cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataag ataagatgta
     4141 ttttaaatgc atctttatgg caaatgctat aacccttacg tagcagaggc ttatttaact
     4201 cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct ggttttgctc
     4261 tgactgcaat gaccacgcca accactaaca gcgcgatacc gcttagcgtc attagcggcg
     4321 gcatttgctg gcgtaacaaa aaggtgtaca gcaagcctgc cagcgtttcg aaaacaatca
     4381 gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag agtgcgccaa
     4441 cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc ggacgggggc
     4501 caaagggtag ggagaagccc ggcgtttgcg tattcagcca gtaacaggcg acgagatagc
     4561 cgatgagaga aaccggcagc gtgaccagcg cctgcgccgt cgcccacatc atcggatgtt
     4621 tgtcgggatt ctcccgcagc cagcgggcgt tgcgcagggc ataccatgcc cagcagacca
     4681 cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa tcggggagtc
     4741 cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa accagtgccg
     4801 gggcgagttt tccccacgcg agtttgccgt cgcgctggct ataaagcaga ttggcaaaaa
     4861 cgggaatcac caccggcagg gtgccaataa tcatcgtgga aacaggcgcg ccagtacgtt
     4921 gaatggcact ggcaaggcag aagtaataga tgaggttgcc catcatggtg agcatcaagg
     4981 cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc caggcaatgg
     5041 gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc gccgggtatt
     5101 ccggcacgat caacggcccg acaaaaataa gcccccacat caaccctgct aacagggcgt
     5161 acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag ttcaggaagc
     5221 agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg atgccacttt
     5281 aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg ttgtcgttgg
     5341 gcggccaatc aatttgctaa gcgttttgct gtcatcaaac agaccgcctt tcgatgcgcc
     5401 aacgtcagaa tccgccagca tatccgccag tccatcaggc agtccgacgc ttttcagtgc
     5461 cgcagcgaaa tcagcttcgc tcaggttttg ataggtaacc tgtttgccgc tctgtttggt
     5521 gagttctgcc gctaactgtg tcagcgtcca ggcactatcg cccgccagtt cgtaaacctt
     5581 gccttcgtga ccggcttcgc taatcacgcg tgccgcagct accgcataat cagcccgcgt
     5641 tgctgaggca attttgccat cgcctgcggc accgataaat acgccgtgct ccagtgctgc
     5701 cggggcgctg gcgaggtagt tttcggtgta ccagccgttg cgcagcaggg tgtaaacgat
     5761 gccagaatca gccagcattt tctccgtctc gatgtgctca tcggcgaggc cgagcgggga
     5821 ggaatcggca tgtagcaggc tggtataagc gataaatttc acgccggccg cctttgcggc
     5881 attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag agatcagcag
     5941 tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt aatcagcctg
     6001 acgcacggta atgccttgtg ctgtcagtgc ctgtgctttt gccggattac gaacgatagc
     6061 cactatttgg ctggcaggaa ccgttttcat caaggattca ataacatagt gaccaagttg
     6121 gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct tgtgtttaaa
     6181 tacgctaaca cttaaactta cttttagtaa gtacgtacaa aaaggtaagt atgagatgag
     6241 tcaggttagc ctgtcgcaac aactgaaaga gggaaatctc ttcgctgaac agtgtccgtc
     6301 gcgcgaggtt ttgaaacacg tcaccagccg ttggggggtg ttgattctgg tggcgctacg
     6361 cgaaggtact catcgcttta gcgacctgcg gcgcaaaatg ggcggggtga gtgaaaagat
     6421 gcttgcgcag tcgttacagg cattagaaca ggatggtttt cttaaccgta tcgcgtatcc
     6481 ggtggtgccg ccgcatgtgg aatatagcct cacgccgctg ggcgagcagg tgagcgaaaa
     6541 ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttag cggtgcggga
     6601 tgaacgtgcg gcataactgc cggatgcgct gcgcttatcc ggactacggt tcatggcttg
     6661 tagggcggat aagatgcgtc atcatcgcat ccgccaataa atgccggatg cggttgatga
     6721 tttgtaggcc gaaaaagaag tgttttactt actcagatcc acctgataaa tcgcaaaccc
     6781 gatatcatcg gtcgcgactt tattcatcgg atactgccct ttctctttaa taaacactgc
     6841 ggctttatct gacggagagg tttcgaaacg gatatccagt ttcttatcgc cagctattgg
     6901 tgctaaacgc cagttgttat ctgccgccgg gtgaatttcc cccgcacgtt tcgactcatc
     6961 agcaatccac gctgccagca ccgagcggtt ctcatccggt gaagcaaaag cgatatggct
     7021 gtcgcccgta ccggcaaatt tgccgccgta agcgcgatag ttattggtgg caacgaggaa
     7081 catggcgttc ggatcaatcg gcttgccgtt aaaggtcagg ttcttaatcc tttccgcatt
     7141 ggcattaatc atctggcact cgccgtcata acgggcgggc tgggtaacat caatctgata
     7201 attcacacca tcaataacat caaagttata ggtgcggaaa ccatcccagt tgatgagtga
     7261 ctgtggtttc gtgctgttag gatcaatctg gttaaactgt ccggcggagc actccagcca
     7321 ctctttcacc tctttaccgc tggctttcac cacaatcagc gtattggggt agagataaag
     7381 atcggcggca ttacggaagg tcaactggcc tttttccacc tccacatagc ttgccgggtc
     7441 atttttgcga ccaccaactt taaacggtgc ggcagctgaa agcactggca gttttgccag
     7501 atccggatcg ccctgaatgt aatgctcgac atacgctttt tgcgcgttgt tcaccacctg
     7561 cacggtcgga tcgtcctgca caagcgccag atagctatac atattgtcgg cagatttacc
     7621 gattggcttg ctgacgaact ggcgtgtggc atcgtgatcg gctttgagtg tttctaccag
     7681 cttgctgtct tccgccgcga gggatttttt attcgcgatg tcgtaaatcg gccgtgcttc
     7741 cgctttcgcc tgcgtcacct gccatttacc gctgtcatta ctgagttgta agtcgaccac
     7801 accaagatga tcgccccaca tgcctggcat taccgccgga acaccattca gcgtgccttt
     7861 cgcgatatca gccccttcga tatcagcaaa atctttacct gggaaaacgg cgtgagcatg
     7921 gccaaacata atggcgttaa cgcccggaat ttcactgagg taataaactg agttttccgc
     7981 catcacttta tacggatcgg cagacagccc ggaatgcgcc agaacgacaa cgacatcggc
     8041 acctttctcg cgcatttcag gcacgtattt gcgcacggtt tcggtaatat cattcaccgt
     8101 cactttcccg gataaattag ctttatccca gcccatgatt tgtggcggga cgacgccaat
     8161 ataaccaatc ttcagcgtct gtttttttcc gtctttatcg accacttcgg tgtctttaat
     8221 taaatacggt gtaaacattg gctgtttggt tctggcgtca atgacgttgg catttacata
     8281 agggaatttc gctcccgcca gcgcattttt cagataatcc agaccgtagt taaactcgtg
     8341 gttgccaagc gtgccgacgg tatagtccag cgtatttaat gccttataga ccgggtgaat
     8401 atcacctgct tttaaacctt tcgccgacat gtaatctgca agcggactcc cctgaatcaa
     8461 atcgccgtta tcgaccagta cgctgttttt cacttcattg cgggcatcgt taatcaggct
     8521 tgcggtacgt accagtccga atttttccgt ggcggtgtct ttgtaataat cgaaatccat
     8581 catgttgcta tgcagatcag tggtttccat gatacgcaga tcgaccgtcg ctgcattcac
     8641 actggcggca atcagcgtgg ccaggagcgt tgcgctaaac ttaatcatca gggacatcct
     8701 tttatcatcg ggaatacgaa agaaaaggga gaataaacgt cttacttata gaacagtgaa
     8761 gaatgccaca attttacgct ttgaaaatga tgacactatc acagttggcg cattcattaa
     8821 cgatagggta taagtaaaac aataagttaa caccgctcac agagacgagg tggagaaatg
     8881 ttagatcaag tatgccagct tgcacggaat gcaggcgatg ccattatgca ggtctacgac
     8941 gggacgaaac cgatggacgt cgtcagcaaa gcggacaatt ctccggtaac ggcagcggat
     9001 attgccgcac acaccgttat catggacggt ttacgtacgc tgacaccgga tattccggtg
     9061 ctttctgaag aagatcctcc cggttgggaa gtccgccagc actggcagcg ttactggctg
     9121 gtagacccac tggatggtac taaagagttt attaaacgta atggcgaatt caccgttaac
     9181 attgcgctca tcgaccacgg caaaccgatt ttaggtgtgg tgtatgcgcc ggtaatgaac
     9241 gtaatgtaca gcgcggcaga aggcaaagcg tggaaagaag agtgcggcgt gcgcaagcag
     9301 attcaggtcc gtgatgcgcg cccgccactg gtcgttatta gccgatccca tgcagatgca
     9361 gagctgaaag agtatctgca acagcttggc gaacatcaga ccacgtccat cggctcttcg
     9421 ctgaaattct gcctggtggc ggaagggcag gcgcagctgt acccgcgctt cggaccaacg
     9481 aatatttggg acaccgccgc tggtcatgcc gtggctgtag ctgccggagc gcacgttcac
     9541 gactggcagg gtaaaccgct ggattacact ccgcgcgagt cgttcctgaa tccggggttc
     9601 agagtatcta tttattaatt aatttatgca gcaggtccat cacttgctgc acctcttcct
     9661 gagtaagcgc cccgtctttg gcccattgca cgcgaccgtc tttatccagc accactacag
     9721 cggaactttc ttcatccagc tgccaggcac cgcgcgcgac gccattgcta tcgacaataa
     9781 actgcgacca gggataaagc tttttattac tctccagact gctgcgcaca aacatgccgg
     9841 aacccggaat tgcgtcgtcg gtgttaacaa tggtggtggt ctggtaacga tcgtgcggta
     9901 actttgctga tttaatcgct tcaatcagcg tcgcgttttt ctcttttgca gaggtgcgac
     9961 cagcaatatg ttgcagtact cgcacttttc ccactagctg cgcgctgttc caggttttgt
    10021 agctaaactg atctttatca agcaccaact cgccacgatc ggtaatgcca atcggcggca
    10081 ctcgctgacc ggtttcgaac tgatgtgcgg aagccatcat cggcaacagc aggcaggtga
    10141 gtgccagaat cttgcgtagg gtcatggtgt ttccttctta tgatatgcag gtgatccgac
    10201 cacttgggtc tgagttttaa tcatatgtgc tatttatcga atttcccgca agtgtgatgc
    10261 cagtttgcgg tcaagcgcac aaatcatatg aaaaatgaat gcttatactg aagacggcgc
    10321 ttcggtaaaa agataattct gaataattgt aacctttagg taaaaaaagt tatacgcggt
    10381 ggaaacattg cccggatagt ctatagtcac taagcattaa aatttgcgcc tcataatagt
    10441 tgggccgatt gtggcaccgc acaggcgtaa tactcagcag gagataacaa tgaaaatttt
    10501 ccaacggtac aacccacttc aggtggcgaa gtacgtaaag atcctgttcc gtggacggtt
    10561 atacatcaag gacgttggcg cttttgaatt tgataagggt aagattctta tcccaaaagt
    10621 gaaggataag ctgcatttgt cagtgatgtc cgaagttaac cgtcaggtta tgcgtctgca
    10681 aacagagatg gcttaaccaa agtgctatgc agtaataaga cggctcctga ttcaggagcc
    10741 gttgatgttt ctgggggtta cgcgacgctt tcttctttat ctttagcgtc aggcaatttt
    10801 ggcgaaaggg cggtggcctt gctgtcgatt cgggtcacca gcagctggtc gatgcggtag
    10861 ttatcgatat ccaccacttc aaatttgtag ccggcgaatt tcaccgaatc ggtgcgtttc
    10921 gggatcttac gcagcataaa catcataaag ccgccgatgg tttcgtagtt gcccgactgc
    10981 gggaactcgt caatatccag cacgcgcatg acgtcgtcaa taggcgtgcc gccgtctatc
    11041 agccatgagt tctcatcacg ggcgacaatt tgctcttcca gcccctgacc gaccagatcg
    11101 cccatcagcg tggtcatcac gtcgttgagg gtgatgatcc ccactaccag cgcgtactcg
    11161 ttcatgatca ccgcgaagtc ttcacctgcg gttttaaaac tttccaacgc ctctgaaagg
    11221 gttaacgtat ccggcacaat cagcgtattg cgaatttgta cgccgctgtt cagtgccagg
    11281 ctttggttag ccagcacgcg gttcagcagg tctttagaat cgacatagcc gatgatgtgg
    11341 tcaatatctt cattacagac gagaaactta gagtgcggat gttccgccac cttattcttc
    11401 agactttgct catcttcgtg gagatcaaac caaatcacgt tttcacgcgg tgtcattgaa
    11461 gacggaacgg tacgggattc cagctcaaag acgttttcaa tcagctcgtg ttcctgttta
    11521 cgtaacacgc ccgccagcgc accggcttcc actaccgcgt agatgtcatc agaagtgata
    11581 tcatctttac gtaccattgg cagtttgaaa atacggaaga ttatgttcgc cagtccgttg
    11641 aagaaccaca ccagcggggt gcaaacgtac aggcagaagc gcatcgggtt gatgatacgc
    11701 aaagccacag cttctggcgc aatcataccg atgcgtttcg gggttaaatc cgcaaacagg
    11761 ataaacatgc cagtcactaa cgagaaagag agaataaagc tcagttgctc agagagttct
    11821 gccgacatat agcgggagaa caggctgtga aaagctggag aaaatgccgc atcaccgacg
    11881 ataccgccga gaatcgccac tgcgttcaga ccgatctgga ccacggtaaa gaacatgccg
    11941 ggattttcct gcatattcag aacgcgttgg gcatttatat tgccttcatc agccagcagt
    12001 ttaagtttga ttttgcgtga ggcggcaagc gagatctcgg acatcgagaa gaacgcactt
    12061 acagcgatca aacagagtat gactaaaata ctgtttaaca tatcttatcc gacctttcag
    12121 gccagatcct cggaagggga agttgattaa tttgtgtgaa atacacattg aaagccgatt
    12181 cgaagaaagt gaaccggcat tttcagtggc tagtatagcg taaggtactg taaagccgcc
    12241 agagggttaa aattcagaca gctgaaaaat gaaaaactgc ctgatgcgct atgccgaaca
    12301 ggcctacgca gtccccgcaa tatattgaat ttggacgatt ttgtaggcct ggcaaggcgt
    12361 ttacgccgta tccggcataa acaaaatgca ctttatcaac aatctacatt tgtacccgta
    12421 acgctatgct tccggcggca gacagacgcc aattccgcca attccacagt aaccatacgg
    12481 gtttttatgc agatattgct ggtggtcatc ttcggcataa taaaacggtg tggcgttagc
    12541 gatttccgtg gtgatgcgac gatcgtcatc ggcggcaagc atcgccgcct gaaaacgttc
    12601 cagactggcg cgagcggcgg catcctgttc tggggtcagc ggataaatcg ctgaacgata
    12661 ctgcgtgccg tggtcattgc cctgacgcat tccctgggcg ggatcgtgat tctcccaaaa
    12721 tacttgtagc aactgctcat agctgatgac ggatggatcg taaacaatgc gcaccgcttc
    12781 ggcatggcca gtatcgccgg agcacacttc ccgataagtc ggatttggcg tatagccgcc
    12841 ggtatagcct gcggcggtgc tgtaaacgcc gggtaactgc cagaacagac gctccacacc
    12901 ccagaaacaa cccatcgcaa aaatggcaat ctccattccg tcaggtacat tggtcattga
    12961 gtgaccgttg accgcatgca gtgtggctac gggcatcggg gtgttacgtc caggcagggc
    13021 atcggcgggg gaaaccagat gctttttatc aaataaactc atggtgtcgc tctcccgaaa
    13081 atcggtcatt ggggttaagg ttgtaacaag aggcgtattt gcacacaata accaccgtga
    13141 ataggtctaa agtaaaacat aagaaatatt ttgggtttag tctgcttttt aatccatatt
    13201 actggatttt tgttaagccg tttaacggcg ttccaggggc aggaaaaaag gatattcagg
    13261 agaaaatgtg cgctatatcc gacagttatg ctgtgtaagc ttactctgct taagcggatc
    13321 tgccgtcgcc gcgaacgtcc gtctacaggt cgaggggtta tcgggacagc tggaaaagaa
    13381 cgttcgcgcg cagctttcta cgattgaaag tgatgaagtg acgccagacc gtcgctttcg
    13441 cgcacgcgtc gatgatgcca tccgcgaagg tctgaaagcg ctgggctatt accagccgac
    13501 cattgaattt gatctccgtc caccgccaaa gaaagggcgg caggtattga tcgccaaagt
    13561 cacgccaggc gtgccggtgt taattggcgg caccgatgtg gtattgcgcg gcggcgcgcg
    13621 gaccgataaa gactatttga aattgctcga tactcgcccg gctattggta cggtactgaa
    13681 ccagggcgat tatgaaaatt tcaaaaagtc cttaaccagc attgcgttgc gtaaaggtta
    13741 tttcgatagc gaatttacca aagcgcagct gggcattgcg ctcggcctgc ataaagcctt
    13801 ctgggatatt gattataaca gtggcgaacg ttaccgcttt gggcatgtga cctttgaagg
    13861 atcacaaatc cgcgatgaat acctgcaaaa tctggtgccg tttaaagagg gcgatgagta
    13921 cgaatcgaaa gatctggcag aactgaaccg tcgactttct gctaccggct ggtttaactc
    13981 ggtggtggtg gctccacaat ttgataaagc gcgcgaaacg aaagtattac cattgacggg
    14041 cgtggtttcg ccgcgaacag aaaacaccat cgaaaccggc gtcggttact ctacggacgt
    14101 gggaccgcgc gtgaaagcga cgtggaaaaa gccgtggatg aactcttacg gtcacagttt
    14161 gaccaccagt accagtattt ccgcgccgga acagatcctc gacttcagct ataaaatgcc
    14221 gctgctgaag aatccgctgg aacaatatta tttggtgcag ggcggtttta agcgcactga
    14281 cctgaacgat accgagtctg actccactac gctggtggct tctcgctact gggatctctc
    14341 cagcggctgg cagcgtgcca ttaacctgcg ctggagtttc gaccacttta cccagggtga
    14401 aattaccaac accacgatgc tgttttatcc tggggtgatg attagccgca cgcgttctcg
    14461 tggtggcctg atgccaacct ggggcgactc gcaacgctac tctatcgact actccaacac
    14521 ggcctggggc tcggatgttg atttctccgt tttccaggcg cagaacgtct ggatccgcac
    14581 actgtacgat cgccatcgtt ttgtgacacg cggcacgctg ggctggattg aaacgggtga
    14641 tttcgacaaa gtaccgccgg atctgcgttt cttcgccggg ggcgatcgca gtattcgcgg
    14701 ctacaaatac aaatctatcg ccccgaaata cgccaacggt gacctgaaag gggcctcgaa
    14761 gctgataacc ggatcgctgg aataccagta caacgtgacc ggaaaatggt ggggcgcggt
    14821 gtttgtcgat agcggcgaag cggtaagcga tattcgccgc agcgacttta aaaccggtac
    14881 cggggtcggc gtgcgctggg aatcgccggt cgggccaatc aaactcgatt ttgccgtacc
    14941 ggtcgcggat aaagacgaac acgggttaca gttttacatc ggtctggggc cagaattatg
    15001 agtttatgga aaaaaatcag cctcggcgtg gttatcgtta tcttactgtt gctgggatcg
    15061 gtggcgtttc tggtgggcac caccagcggc ctgcatctgg tatttaaagc ggcggatcgc
    15121 tgggtgccag gactggatat tggcaaggtc accggcggct ggcgtgatct caccttgtct
    15181 gacgttcgtt atgagcagcc aggcgtggcg gtaaaagcgg gcaatctgca tctggcggtc
    15241 gggcttgagt gcctgtggaa cagtagcgtt tgtattaatg atctggcgct gaaagacatt
    15301 caggtcaaca tcgacagtaa aaaaatgcct ccttctgaac aggttgaaga agaggaagat
    15361 agcggtccgt tggatctctc catgccgtat cccattaccc tgacacgggt ggcgctggac
    15421 aacgtcaaca tcaagattga tgacaccacg gtatcggtga tggacttcac ctccggcctg
    15481 aactggcagg agaaaaccct gaccctgaaa ccgacatcgc tgaaaggcct gttgattgct
    15541 ttgccgaaag tggcggaagc ggcgcaggaa gaagtggtcg aaccgaaaat tgaaaatcca
    15601 cagccggagg aaaagccgct cggcgaaacg ctgaaagatc tcttttctcg cccggtattg
    15661 ccggaaatga ccgacgtgca tctgccgctc aacctgaaca ttgaagagtt taagggcgag
    15721 caactgcgcg tgacgggcga cacggacatc accgtgcgca ccatgctgct gaaagtgagc
    15781 agtatcgacg gcaataccaa actggacgcg ctggatatcg actccaacca ggggatcgtc
    15841 aacgccagcg gcacggcgca gctgtcagac aactggccgg tggatatcac cctcaacagc
    15901 acactgaacg tggagccgtt gaaaggtgaa aaggtgaagc tgaaagtggg cggtgcgctg
    15961 cgcgaacagc tggagatagg cgttaacctt tccggtccgg tggatatgga tttacgcgcc
    16021 cagacgcgac tggcggaagc cggattgccg ctcaacgtgg aagtgaacag caaacagctt
    16081 tactggccgt tcactggtga gaagcagtat caggcggatg atctgaaact gaaacttacc
    16141 ggcaaaatga ccgattatgc gatctctatg cgcacggctg ttaagggcca ggagatcccg
    16201 ccagccacca ttacccttga tgccaaaggt aatgaacagc aggtcaatct cgacaaactt
    16261 accgtcgcgg cgctggaagg gaaaacggaa cttaaggcgt tgctcgactg gcagcaggcc
    16321 attagttggc gcggtgagct aacgcttaac ggtattaaca ccgccaaaga gatcccggag
    16381 tggccgtcga aactcaatgg cttgattaaa acccgcggta gcctgtacgg cggcacctgg
    16441 cagatggagg tgccagaact gaagctgacc ggtaacgtca aacagaacaa agtgaacgtt
    16501 gacggcacgc tgaaaggcaa cagttatatg cagtggatga tcccagggct gcatctggaa
    16561 ctggggccaa acagtgccga agtgaaaggc gagctggggg taaaagatct caatcttgat
    16621 gccaccatca acgcgccggg gctggataac gcgctgccgg ggcttggcgg tacagcgaaa
    16681 gggttggtga aagtacgcgg cacggtggaa gcgccacaac tactggcaga tatcaccgcg
    16741 cgcggcctgc gctggcagga actttccgtg gcgcaggttc gcgtggaagg cgatatcaaa
    16801 tccaccgacc agattgcggg taaactcgac gtacgcgttg agcaaatttc gcagccggat
    16861 gtaaatatca acctcgtcac cctgaatgcc aaaggcagcg aaaagcagca cgagctacag
    16921 ttgcggattc agggcgagcc tgtctccggg cagcttaatc tggcaggaag ttttgatcgc
    16981 aaagaagaac gctggaaggg aactcttagc aatacccgct tccagacgcc ggtcggcccg
    17041 tggtcgctga cccgcgatat tgcgctggat taccgcaata aggagcaaaa aatcagcatc
    17101 gggccacact gctggcttaa cccgaatgcg gaactgtgcg tgccgcaaac tatcgatgcg
    17161 ggtgccgaag ggcgtgcggt ggtgaatctc aaccgcttcg acctggccat gctgaaacca
    17221 tttatgccag aaaccactca ggccagcggt atcttcacgg gtaaagcgga cgttgcctgg
    17281 gacaccacga aagaggggct gccgcagggc agtatcaccc tttcggggcg taatgtgcag
    17341 gtgacgcaaa ccgtcaacga tgcggcgctg cccgtggcgt ttcagacgct gaatctgacg
    17401 gcggaattgc gtaacaaccg tgccgaattg ggctggacca tccgcctgac caataacggc
    17461 cagtttgatg gacaggtgca ggtgaccgat ccgcaaggcc gccgtaatct tggtggcaac
    17521 gtcaatatcc gtaacttcaa ccttgcgatg ataaacccca tctttacccg tggggaaaaa
    17581 gcagcgggga tggtgagtgc caacttgcgt ctgggtggtg atgtgcaaag cccgcagttg
    17641 tttggtcagc ttcaggttac gggtgtggat atcgacggca actttatgcc gtttgatatg
    17701 caaccgagcc agcttgcggt caactttaac ggtatgcgct cgacgcttgc cggtacagta
    17761 cggacccagc agggtgaaat ctacctgaac ggtgatgccg actggagcca gattgaaaac
    17821 tggcgggcgc gggtaacggc gaaaggcagt aaagtgcgga tcaccgtgcc gccgatggta
    17881 cgaatggatg tatcgccaga tgttgtattc gaggctacac caaacctgtt taccctcgat
    17941 ggtcgcgtgg atgtcccgtg ggcgcgcatc gtggtgcatg agctgccgga aagcgcggtg
    18001 ggcgtctcca gcgatgtggt aatgcttaac gataacctgc aaccggaaga ggcaaaaacg
    18061 gcgtcgattc cgattaacag caacctgatt gtccacgtcg gcaacaatgt gcgcattgac
    18121 gcctttggcc tgaaagcgcg gctgacgggc gatctcaacg tcgttcagga caaacaaggg
    18181 ctgggcctga acgggcagat caacatccct gaagggcgct tccatgccta tggtcaggat
    18241 ctgattgtgc gtaagggtga gttactgttc tctggtccgc cggatcaacc gtatcttaat
    18301 atcgaagcta ttcgtaaccc ggatgctaca gaagacgacg taatcgccgg agttcgcgtc
    18361 actggtctgg cggacgaacc gaaagcggag atcttctctg acccggccat gtcgcaacag
    18421 gctgccttgt cttatttgct acgtggacaa gggctggaga gcgatcagag cgacagtgcg
    18481 gcgatgacct cgatgctgat tggtctgggg gttgcacaaa gtggtcagat tgtgggtaaa
    18541 atcggcgaga cgtttggcgt aagcaattta gcgctcgaca cccagggagt aggcgactcc
    18601 tcccaggtgg tggtcagcgg ctatgtattg ccaggtctgc aagtgaaata cggcgtgggt
    18661 atatttgact ctatagcaac actcacgtta cgttatcgcc tgatgcctaa gctatatctg
    18721 gaagccgtgt ctggtgtaga ccaggcactg gatttgctct atcagttcga gttttagcaa
    18781 tgcgaatatt tgtctacggc agtttacgcc acaaacaagg caacagtcac tggatgacca
    18841 atgcccagtt actgggcgat ttcagtatcg ataactacca gttgtatagc ctgggccact
    18901 atccaggcgc agttccgggg aacggaacag tacacggtga agtttatcgt attgataacg
    18961 ccacgctggc cgaacttgat gccttgcgca ccaggggcgg tgaatacgcg cgccagttga
    19021 ttcagacgcc gtacgggagt gcatggatgt acgtttatca acgacccgtc gatggattaa
    19081 agctaattga aagcggcgac tggttagaca gggataagta ataataaaag cacgccacct
    19141 tcgggtggcg cgaaaattga tgtaatgcaa tgaaaaataa aggattattt ttctcgttgt
    19201 ccacattttg ccatagcgat acatcttagc ccgtaatgat tcgcgggcta agcatataca
    19261 aaatgttaag cctgatacgg gggtcctatg tttgataggg aaatgataaa cgaaaataaa
    19321 gaggcgtttt ttctgtatgt taacggtaga ggggagttgt cggctcaatg cgtgataaag
    19381 gtggctgaaa aaggggatta tttacaagga ttctctttaa ctcgtcgcgc atttcgtacc
    19441 tt
//
LOCUS       DAZKJR010000098        19227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKJR010000098 DAZKJR010000000
VERSION     DAZKJR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.099"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..1657
                     /gene="pdeN"
                     /locus_tag="ACHOWS_003489"
     CDS             101..1657
                     /gene="pdeN"
                     /locus_tag="ACHOWS_003489"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFQ0049016.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFTDLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            1739..3553
                     /gene="yejA"
                     /locus_tag="ACHOWS_003490"
     CDS             1739..3553
                     /gene="yejA"
                     /locus_tag="ACHOWS_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0049017.1"
                     /translation="MIVRILLLFIALFTFGAQAQTIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNAGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRLEND
                     AKNWVTRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            3554..4648
                     /gene="yejB"
                     /locus_tag="ACHOWS_003491"
     CDS             3554..4648
                     /gene="yejB"
                     /locus_tag="ACHOWS_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFQ0049018.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNTGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            4648..5673
                     /gene="yejE"
                     /locus_tag="ACHOWS_003492"
     CDS             4648..5673
                     /gene="yejE"
                     /locus_tag="ACHOWS_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0049019.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            5675..7264
                     /gene="yejF"
                     /locus_tag="ACHOWS_003493"
     CDS             5675..7264
                     /gene="yejF"
                     /locus_tag="ACHOWS_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFQ0049020.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGKPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(7268..7612)
                     /gene="yejG"
                     /locus_tag="ACHOWS_003494"
     CDS             complement(7268..7612)
                     /gene="yejG"
                     /locus_tag="ACHOWS_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFQ0049021.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(7946..9136)
                     /gene="bcr"
                     /locus_tag="ACHOWS_003495"
     CDS             complement(7946..9136)
                     /gene="bcr"
                     /locus_tag="ACHOWS_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFQ0049022.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(9164..9859)
                     /gene="rsuA"
                     /locus_tag="ACHOWS_003496"
     CDS             complement(9164..9859)
                     /gene="rsuA"
                     /locus_tag="ACHOWS_003496"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFQ0049023.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            10009..11769
                     /gene="radD"
                     /locus_tag="ACHOWS_003497"
     CDS             10009..11769
                     /gene="radD"
                     /locus_tag="ACHOWS_003497"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0049024.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAVTRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            11894..12178
                     /gene="rplY"
                     /locus_tag="ACHOWS_003498"
     CDS             11894..12178
                     /gene="rplY"
                     /locus_tag="ACHOWS_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFQ0049025.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(12317..13324)
                     /gene="yejK"
                     /locus_tag="ACHOWS_003499"
     CDS             complement(12317..13324)
                     /gene="yejK"
                     /locus_tag="ACHOWS_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFQ0049026.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            13506..13733
                     /gene="yejL"
                     /locus_tag="ACHOWS_003500"
     CDS             13506..13733
                     /gene="yejL"
                     /locus_tag="ACHOWS_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFQ0049027.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            13753..15513
                     /gene="yejM"
                     /locus_tag="ACHOWS_003501"
     CDS             13753..15513
                     /gene="yejM"
                     /locus_tag="ACHOWS_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFQ0049028.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            15588..15664
                     /locus_tag="ACHOWS_003502"
     tRNA            15588..15664
                     /locus_tag="ACHOWS_003502"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15622..15624,aa:Pro,seq:ggg)
     gene            complement(15790..16549)
                     /locus_tag="ACHOWS_003503"
                     /pseudo
     CDS             complement(15790..16549)
                     /locus_tag="ACHOWS_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            16869..17516
                     /gene="narP"
                     /locus_tag="ACHOWS_003504"
     CDS             16869..17516
                     /gene="narP"
                     /locus_tag="ACHOWS_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFQ0049029.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(17551..18603)
                     /gene="ccmH"
                     /locus_tag="ACHOWS_003505"
     CDS             complement(17551..18603)
                     /gene="ccmH"
                     /locus_tag="ACHOWS_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFQ0049030.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKAAPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(18600..19157)
                     /gene="dsbE"
                     /locus_tag="ACHOWS_003506"
     CDS             complement(18600..19157)
                     /gene="dsbE"
                     /locus_tag="ACHOWS_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFQ0049031.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKL
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT         4669 a         5022 c         4791 g         4745 t
ORIGIN      
        1 ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc tatagtccgg tcattgttat
       61 cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc atgttcatac gcgctcccaa
      121 ttctggacgt aagctcctgc ttacctgcat tgttgcaggc gtgatgattg cgatactggt
      181 gagctgcctt cagtttttag tggcctggca taagcacgaa gtcaaatacg acacactgat
      241 taccgacgta caaaagtatc tcgataccta ttttaccgac ctgaaatcca ctactgaccg
      301 gctccagccg ctgaccttag atacctgcca gcaggctaac cccgaactga ctgcccgcgc
      361 ggcgtttagc atgaatgtcc gtacgtttgt gctggtgaaa gataaaaaaa cattctgttc
      421 atctgcgact ggtgagatgg acattccact aaaagaattg attccggcgc tcgacattaa
      481 taaaaatgtc gatatggcga tcttacccgg tacgccgatg gtgccgaaca aacccgcaat
      541 cgtcatctgg tatcgcaacc cgttgttgaa aaatagtggc gtgtttgccg cgctgaatct
      601 caatctgacg ccttctctct tttatagttc acggcaggaa gattacgatg gcgtcgccct
      661 cattattggt aatactgcgc tatctacctt ttcttcacgt ttgatgaacg ttaatgaatt
      721 aaccgacatg ccggtccgtg aaactaaaat tgcgggcatt cctctgaccg ttcggcttta
      781 tgcagatgac tggacatgga acgatgtgtg gtacgcattt ttactgggtg gcatgagtgg
      841 aactttcgtt ggacttctct gctattacct gatgagtgtg cgtatgcgcc caggcagaga
      901 aatcatgacc gccatcaagc gcgaacaatt ttacgtggtg tatcaaccgg tggtggatac
      961 acaagctttg cgggtaacgg gcctggaagt actgctacgc tggcggcatc cagtagcagg
     1021 agaaatcccc ccggatgcct tcattaactt tgccgaagcg caaaagatga ttgtgccact
     1081 gactcagcac ctgtttgagt tgattgcccg cgatgccgca gaattagaaa aagtactgcc
     1141 ggtaggcgtc aaatttggca ttaacattgc gccggcccac ttgcacagcg aaagctttaa
     1201 agcggatatc cagaaactgc tcacttccct gcccgcacac catttccaga ttgtgctgga
     1261 aattaccgag cgcgatatgc tgaaagagcg agaagccaca caactcttcg cctggctgca
     1321 ttcggtcggc gtagaaattg ctattgatga cttcggcacc gggcacagcg cgcttatcta
     1381 tcttgagcgt tttacgctcg attatctgaa aattgatcgt ggatttatca acgccatcgg
     1441 tacggaaacg atcacttcac ccgtacttga cgcggtgctg acgctggcga aacgcctcaa
     1501 tatgctgacg gttgctgaag gggtcgaaac gccagaacag gcacgatggc taagcgaacg
     1561 cggcgttaat ttcatgcaag gctactggat tagtcgcccg ttaccgctgg acgattttgt
     1621 tcgctggctg aagaaaccgt atacgccgca gtggtaaggt gtgcttacgt cccttattat
     1681 tcatagtgaa agcatgccgg attgcggcta atgatgagta aaaggaaatc cgttgcagat
     1741 gattgtgcgc atactgctgc tgtttatcgc tctgttcacc tttggtgcgc aggcgcagac
     1801 tatcaaggaa agctatgcct ttgccgtact gggcgaaccc cggtacgcat ttaatttcaa
     1861 ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg caaataacgt tgtctgcgct
     1921 cggcaccttc gataatttca accgctatgc actgcgcggc aatgcgggcg cacgcaccga
     1981 gcagctgtac gacacgctat ttacgacttc cgatgacgaa ccaggcagtt attacccgct
     2041 gattgctgaa agcgcacgct atgctgacga ttattcctgg gtggaggtcg ctattaatcc
     2101 acgcgcccgt tttcatgatg gttcgcccat tactgcccgc gatgtagagt ttacttttca
     2161 aaaatttatg accgaaggcg tgccgcaatt tcgtctggtc tacaaaggca ccaccgtcaa
     2221 agccattgcg ccgttaaccg tgcgcattga gttagcgaaa cccggcaaag aagatatgct
     2281 gagcctgttt tcgctgccgg tctttccaga aaagtactgg aaggatcaca aacttagcga
     2341 cccgctcgcc acgcctccgc ttgccagtgg tccgtaccga attacgtcct ggaaaatggg
     2401 gcaaaatatt gtctattccc gcgtgaaaga ttactgggca gcaaacttac cggtaaaccg
     2461 tggacgctgg aatttcgaca ccattcgcta cgattattac ctcgatgata atgtcgcctt
     2521 tgaagcgttt aaagccggtg cctttgattt gcgtttggaa aacgacgcca aaaactgggt
     2581 cacgcgctat accggtaaaa atttcgataa gaaatacatc atcaaagatg agcaaaagaa
     2641 cgaatcagcc caggatacgc gctggctggc gtttaatatc caacgtccgg tattcagcga
     2701 tcgccgggtg cgggaagcaa tcactctcgc ctttgacttt gaatggatga acaaggcgtt
     2761 gttttataat gcctggagtc gcacgaacag ttattttcag aataccgaat atgcggccag
     2821 aaattacccc gacgccgcgg agctggtgct tctggcacca atgaaaaaag atctaccgcc
     2881 agaagtcttc acgcaaatct accagccgcc ggtatccaaa ggcgatggct acgatcgtga
     2941 caacctgtta aaagccgaca aacttctcaa cgaagcgggc tgggtgctga agggtcagca
     3001 acgcgtaaat gccacaacgg gtcagccgct cagctttgag ttattgctac cttcgagcag
     3061 caatagtcag tgggtattgc cgttccagca cagcctgcaa cggctgggta tcaacatgga
     3121 cattcgcaaa gtggataact cgcaaatcac caaccgcatg cgcagtcgcg actatgacat
     3181 gatgccgcgc gtatggcggg cgatgccgtg gcccagttcc gatttacaga tttcctggtc
     3241 atcggaatat atcaattcca cttataatgc ccccggcgtg caaagcccgg ttatcgactc
     3301 gctgatcaac caaattattg ccgcgcaggg aaataaagaa aaattactgc cgttggggcg
     3361 agcactggat cgcgtattaa cgtggaatta ttacatgctg ccaatgtggt acatggcgga
     3421 agaccgtctc gcctggtggg ataaattctc ccaacccgct gtacgccctg tttacagcct
     3481 gggtattgat acctggtggt atgacgttaa taaagcgacg aaactgccgt cagccagaca
     3541 acagggagag tagatgggcg cttatctgat tcgccgtctg ttgctggtga tcccaacgtt
     3601 atgggcgatt atcactatca actttttcat cgtgcaaatt gcgcctggcg gtccggtcga
     3661 ccaggccatc gccgccattg agtttggtaa tactggtgta ttacccggtg caggcggtga
     3721 aggtgttcgc gccagccatg cacaaacggg cgtcggtaat atcagcgaca gtaattaccg
     3781 tggcggacgc ggattggatc cagaagtgat cgccgagatc actcatcgct acggtttcga
     3841 taagccgatc cacgaacgtt acttcaaaat gctctgggac tacatccgtt ttgattttgg
     3901 cgacagcctt tttcgcagcg cctcggtgct gacgctgatt aaagacagtc tgccggtctc
     3961 catcaccctc ggattgtgga gcacgctgat tatctatctg gtgtcgattc cgttaggcat
     4021 tcgcaaagct gtttataatg ggagccgttt tgacgtctgg agtagtgcat ttatcatcat
     4081 cggctacgcc attccggcct ttttgtttgc catcctgctg attgtcttct tcgcgggcgg
     4141 cagctatttc gacctgttcc ctctgcgcgg cctggtttcc gctaactttg attcgctgcc
     4201 gtggtatcag aaaatcaccg attatctgtg gcatatcacg ctgccggtgc tggcgacagt
     4261 gattggcggc tttgcggctc tgaccatgct gacaaaaaac tcattccttg atgaagtgcg
     4321 taagcaatac gtggtgacgg cacgtgcgaa aggggtaagt gaaaaaaata ttctctggaa
     4381 acatgtgttc cgcaacgcca tgctgctggt gattgccggt tttccggcga cgtttatcag
     4441 catgtttttt accggctcgc tgctgattga ggtgatgttt tcactcaatg gtcttggctt
     4501 actgggctac gaagcgaccg tctcgcgcga ttatcctgta atgtttggta ccttgtatat
     4561 tttcaccctg attggcctgc tgctgaatat tgtcagtgat atcagctata cgctggtcga
     4621 tccgcgtata gattttgagg gacgttaatg tcgcgactca gccccgtcaa tcaggcccgt
     4681 tgggcgcgtt ttcgtcataa ccgtcgcggc tactggtcgt tatggatttt cctcgtcttg
     4741 tttggtttga gtttgtgttc tgaacttatc gccaacgata aaccgttgct ggtgcgttat
     4801 gacggcagtt ggtatttccc gttgttgaaa aactacagcg aaagcgattt tggcggcccg
     4861 ctggcaagtc aggctgatta tcaggacccg tggctgaaac aacggctgga aaataacggc
     4921 tgggtactgt gggcaccgat tcgctttggt gctaccagta tcaactttgc taccgataag
     4981 cccttccctt ctccaccctc ccggcaaaac tggctgggaa cggatgccaa tggcggcgat
     5041 gtgctggcgc gcattctcta tggcacgcgg atctcggttc tgtttggcct gatgctgact
     5101 ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac aaggctatta cggcggtaaa
     5161 gtcgatctct gggggcaacg ctttattgaa gtatggtcag ggatgccgac gctgtttttg
     5221 attattttac tttccagcgt cgtacagcct aacttctggt ggctgctggc aattaccgtc
     5281 ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg agtttttacg tacccgtaat
     5341 ttcgactaca tccgcgcggc gcaggcgctt ggcgtcagcg atcgcagtat catcctgcgt
     5401 catatgttgc caaatgccat ggtcgctacc ctcacctttt taccgtttat tttatgtagt
     5461 tcgataacca ccctgacctc actcgatttc ctcggcttcg gtctgccgct cggttcaccg
     5521 tcactcggcg aactgctgtt acaagggaaa aataaccttc aggccccgtg gcttgggatc
     5581 accgccttct tgtcggtggc gatattgttg tctttgctga tctttattgg tgaagccgtc
     5641 cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg caaactctgt tagcgattga
     5701 aaatttgtcg gtgggttttc gccatcagca aaccgtacgc acagtagtca atgatgtttc
     5761 acttcagatt gaggctggcg aaacgctggc gctggtgggt gagtcaggtt caggcaaaag
     5821 cgttaccgcg ctgtcaattt tacgcctgct cccttccccg ccggttgaat atctctccgg
     5881 cgatattcgt tttcatggcg aatcgctgct tcatgccagc gatcaaacgt tgcgcggtgt
     5941 acgcggtaat aagatcgcca tgatttttca ggagccgatg gtgtcgttaa atccattaca
     6001 taccctggaa aaacagcttt atgaagtact ttcactccac cgcgggatgc gtcgggaagc
     6061 ggctcgtggc gaaattctta actgccttga tcgcgtcggt atccgccagg cggcaaaacg
     6121 gctgacagat tatccgcatc agctctccgg cggcgaacgc cagcgggtga tgattgcgat
     6181 ggcgctgtta acgcgaccgg aattattaat tgccgatgaa ccaaccaccg cgctggacgt
     6241 ctctgtccag gcgcagattt tacagctgtt gcgcgaactg caaggcgagc tgaatatggg
     6301 tatgctgttt attactcata acctcagcat tgttagaaaa ctggcccacc gcgtggcggt
     6361 aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct acgctatttg cctcacccac
     6421 tcatccttac acacaaaagc tactcaacag tgaaccgtca ggcgacccgg tcccattgcc
     6481 agaacctgcc tcaacgttgc tggatgttga acagcttcag gttgccttcc ccattcgcaa
     6541 agggattttg aagcgcattg tggatcataa tgtggtggtg aaaaacatca gttttacgct
     6601 acgagcgggt gaaacactgg gtttagtggg cgagtctggt tccgggaaaa gcacgacggg
     6661 actggcgctg ctgcgactga ttaactctca gggcagcatc gtctttgacg gtaagccact
     6721 gcaaaattta aatcgccgcc agctgttacc tattcgtcat cgcattcagg tggtatttca
     6781 ggatccaaac tcctcgctca acccacgact caacgttttg cagattattg aggaaggctt
     6841 acgggttcac cagccgacgc tttctgccgc gcaacgcgaa caacaagtga tagccgtgat
     6901 gcatgaagtg ggattagatc ctgaaacacg ccaccgttat ccggcggagt tctctggtgg
     6961 tcagcggcaa cgtattgcga ttgccagggc gttaattctt aagccctcgc tgatcattct
     7021 tgatgaacca acatcatcac tcgacaaaac ggtgcaggcg caaatattga cgctattgaa
     7081 atcattgcaa caaaagcatc aactggccta tttgtttatc agccacgatt tgcacgttgt
     7141 ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg gaagtagtgg aacaaggacc
     7201 gtgcgcgcgc gtgtttgccg caccgcagca ggagtatacg cgtcagctac tggcgttgag
     7261 ctgacgctta aaaaggattg tagtttgaaa agggttcggc aattgccaca ccaaaatttt
     7321 tcaatcggca tgttgcagca aattcgtcct ggcgatttac aaacaggcac ggctcacctt
     7381 cacactccag caccgaacat ggaacttcga tgtcgcttaa cgcctggctt agtttgtaca
     7441 tcaccgacca tgcattatca ccatccgggc taagcaattt aagcgccacc aggctgttgt
     7501 caccgcacgg tccattttgc ggaatcggtt caaccttcga acctgcgaaa ccagcagacc
     7561 agcgatagtt ctgcggcagt cgatggacga ttgagagttg taatgaagtc acttattttc
     7621 cccggaagta cattttactt cacaatttgt ttacatcaat tttaacacat catcaacaat
     7681 ccgtctttat acagatcgta aagattgatg cctcgcctgc gtggcctcta tggctctgat
     7741 ttaaataata tctgcgggcg tggtcacgtt tgcggggcta tatgtacccg tttctgcgat
     7801 ctaactcaac ctttttaact acaatgatgt gactttttac ataaattgat tttacataaa
     7861 ataaacatat atcgggggaa tgatagattt gtggttgaca tgcatcaaca aaggaagcct
     7921 tttagcttcc tcgttgtgca atagatcacc gttttttcgg ccgactggcg tacaaacaga
     7981 agagaatgga gctggttgcg cagaatgcaa ttgaccaaat catcggccat gcagagttaa
     8041 aggtcgcaag agaaagtaat gcgccaacaa ttgcccctat gccgaaacgg aaggttcctg
     8101 ccagcgaaga tgccgttccc gccatatggg gaaactcatc aagaatgacc gccatcgcat
     8161 tggatgacac catcgacacg cagcccacaa acgccgcaac gccaaccacc agcgaccaga
     8221 atcccagccc cagcagtgca ctgatgacca tccacgctgc cataataaac tgtatccaca
     8281 accccgagcg gaacatattt aacgcgccaa tacggcggac gaagcggctg ttaaagatgg
     8341 tcatcacgaa cagaaaaaga atgtttagcg caaagtaata accaaagttt tccggcgcga
     8401 cgtggttgat ttcaatgtag acaaacggtc cggcgcttaa gaatgagaac atcccggcaa
     8461 agctgaaacc gctggccagc atataactca ggacacgctt atgacggaac agcgccgcaa
     8521 agttaccaat agtggtacga atgtgaaaag gctggcgacg ctccggtggt aaggtttctt
     8581 tgatcagaaa gaagatcatt gccgaagcca gaatcgccgc cacggcgagg atccagaaga
     8641 tgtaatgcca gctcagccac accagcaccc agccgccaac tatcggtgcc atcagcggtg
     8701 caatggtcgt caccagcatg acaaacgaca tcatccgtga gaactcttct ttcgggtaga
     8761 tatcacgcat caaagcgttg ataaccacgc tggccgcagc cgcagccagc ccgtggaaga
     8821 aacgcatcac aatcagctga tcgatggttt gtgccaacgc acacgccacc gcggcggcgg
     8881 caaacaccag cgtaccgcca agaaccaccg gcttacgccc gaagctgtct gccatcggcc
     8941 cgtagattaa ctgccccaac gcaaagccca gaatataagt actgagggtc atctgcgtac
     9001 tgcccgccgg tacaccaaac tgagctgaaa ttaccggtag cgcaggcaga tacatatcaa
     9061 tcgacagcgg catcaacatg gccagcaggc caaggataaa aacaatagca aacgacgaat
     9121 gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa aagttagacg acgctggcaa
     9181 tttcttcttc agttaacgga cgatattcac cgggagctaa atcagcatcc agcgtaatgc
     9241 cgccaatacg ttcacgatgc agctcaacca catggttacc cacggcggcg aacatgcgtt
     9301 tcacctgatg ataacgccct tcgctgatgg tcagacgaac ctgcgttggg gtaatcactt
     9361 ccagcaccgc cggcttagtg agatcttttt cgttatgcag ctgcacgcct ttagcaaatt
     9421 gctctgccgt atcgtcagct acgggtgatt ccagcgtcac cagataggtc ttctcgcaat
     9481 gatggcgcgg ggaagtaatg cggtgcgacc actgaccatc atctgtcatt agtaccagac
     9541 cggtggtatc gatatccaga cgccctgcag cgtgcagttt ccacgctacc ggttcatcaa
     9601 gaaaatagag caccgttggg tgatccgggt cgtccgtgga gcaaacatag ccctgaggct
     9661 tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag cgggttgcca tcgtaagcga
     9721 catcatgttc agggagcagt ttgaacgctg cattacggac gatttcgcca tcgacggtga
     9781 cacgattgcc gcggatttca cgcccggcaa tagcacggct aacgccgagt tgctgtgcga
     9841 taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa aaacggaggt cgggtaatga
     9901 cccgaaaatc tgaactgttg tctgcccagt atagcggtct aacaacatcc ctcaagggaa
     9961 aaagatccat ggcatactat tagcagaata atctacctac gcgagaccat gatttttaca
    10021 cttcgcccat atcagcaaga agccgtggat gccacgctca accattttcg tcgtcataaa
    10081 acccctgccg ttatcgtgct gcccaccggt gcaggtaaaa gcctggtgat agcggaactg
    10141 gcgcggctgg cgcgtggtcg cgtgctggtg ctggcacacg ttaaagaact ggtggcgcaa
    10201 aaccatgcaa aatatcaggc gctggggctg gaagccgata tttttgccgc cgggctaaag
    10261 cgcaaagaga gccacggtaa agtggtattt ggcagcgtgc agtcggtcgc ccgtaatctt
    10321 gatgcctttc agggtgaatt ttcgctgttg attgtcgatg aatgtcaccg tattggtgac
    10381 gatgaagaga gccagtatca gcaaatcctc actcacctga ccaaagtgaa tccccactta
    10441 cgcctgctgg ggctgactgc cacgcctttt cgactgggca aaggctggat ttatcagttc
    10501 cattatcacg gcatggtacg cggcgatgag aaagcccttt tccgtgactg catttatgag
    10561 ctaccgctgc gttatatgat taaacacggc tatctgacgc cgccagaacg actggatatg
    10621 ccagtagtgc aatacgattt cagccgcttg caggcacaga gtaacgggct attcagcgaa
    10681 gccgatctca accgcgagct gaaaaaacag caacgcatta ccccgcacat catcagccag
    10741 attatggagt ttgctgtaac gcgcaaaggg gtgatgattt tcgccgccac cgttgaacac
    10801 gcaaaagaga ttgtgggact gctgcctgcc gaagatgctg cactgattac tggcgacacc
    10861 cccggcgctg agcgcgatgt gttaattgaa gattttaaag cccagcgttt tcgctatctg
    10921 gtcaacgtcg cggtactgac caccggattt gacgccccgc acgtcgatct tatcgccatt
    10981 ctgcgcccta ccgaatcagt gagtctttac caacaaattg tcgggcgcgg tctgcgtctc
    11041 gctcccggca agactgattg cttaattctt gattatgcgg gtaaccctca cgatctctac
    11101 gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg ttccggtaca ggttttctgc
    11161 cctgcctgcg gttttgccaa caccttttgg gggaaaacga ccgccgacgg gacattgatt
    11221 gaacactttg gtcgtcgctg tcagggatgg tttgaagatg acgacggaca tcgcgaacag
    11281 tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta acgctgaaaa tgatattgcc
    11341 gcccgccgct gccgcgaatg tgacacggta ctggttgatc cggatgatat gttaaaagcg
    11401 gcgctacgac tgaaagacgc gctggtatta cgctgtagcg gcatgtcttt gcagcatggg
    11461 cacgacgaga aaggcgaatg gttgaaaatc acctattacg atgaagacgg cgcggatgtg
    11521 agtgagcgtt tccgtctgca aacgcccgcc cagcgtaccg ccttcgaaca actttttatc
    11581 cgtccgcata cgcgcacacc gggcatccct ctgcgctgga tcaccgccgc cgatatcctc
    11641 gcccagcaag cattactgcg acacccggat tttgtcgtcg cccgcatgaa aggtcagtac
    11701 tggcaagtgc gtgaaaaagt gttcgattac gaaggtcgtt ttcgtcgggc gcacgaatta
    11761 cgcggttaat ggtagttttg attgatgtat aaggcgattg agtatagaat ctcgcccgct
    11821 tttgcatacg caaagcagat cacttacctg ttgctgggtc gcctgtagca ggattaattt
    11881 aagagagaaa gaaatgttta ctatcaacgc agaagtacgt aaagagcagg gtaagggtgc
    11941 gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc atctacggtg gcaaagaagc
    12001 gccgctggct gtcgaactgg atcacgacaa agtcatgaac atgcaagtta aagctgaatt
    12061 ctacagcgaa gttctgacca tcgttgttga cggtaaagaa atcaaagtta aagctcagga
    12121 cgtacagcgt cacccgtaca aaccgaagct gctgcacatc gacttcgttc gcgcttaatt
    12181 gctgaataag ttgtaaaaaa ccccgctccg gcggggtttt ttgtctctgc aaattatgcc
    12241 tgattacggt attgctattt tttgcaggcc agataaggcg ttcacgccgc atctggcatt
    12301 cagaacacaa aaccgattaa ttgccgccag acgtccggcg ctgcaattga tcgcgcaagt
    12361 tcggcggcgt gcctttaatg gtcagggtat cggttgctgg atcccagaag atgcgttcac
    12421 ctaacagcat cgcatcaaag ttaatagtca agccaccgcc gctaccggca aacttcgtca
    12481 actgacgcag tgtgctacgg tctgccggga agctctcttc cagttcgtag cctttttcag
    12541 ccgcaaactc cgtgaagctc acttcgctga cgcctgccag ctctttcgac agcgattcca
    12601 gttcaatctc ttcacctgcc tgcagctgct cattacagta gctataaacc tgctggcgca
    12661 cgttctgtcg ctctgcttta tccagttgtg cttcggcagt gaaatcatcg accgcttgca
    12721 acagcccacg gttttgcgct ttcgcgttta acccttcgct ggcaccgagg aaatccataa
    12781 agaaatccgc cactttgcgc cctactcgcc ctttcaggaa agtgagatag cgggtcgact
    12841 ctggattggt ttcccactcg gttaaatcga tacgcgcaac aatatccgca tgattgatat
    12901 caagataatg ggttgggttg atgtcgagat tttcgttaac acgcatactg ctcaggttgc
    12961 tcagaactgc caccagcaga tactccaccg ccagatagcg atagtggcaa aacagcacga
    13021 atccaccgtc agcaaaagga tatttcgcta attcgtcacg caggcgaccg gttgccgcgc
    13081 ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa gcgcagcgtc tgcgccagtt
    13141 cactctcttc gctaaacaag ccgtaagctt tatttttggc gctatagacc cgatgcagtt
    13201 ctgccaccat ctcaacgacg gtttccgtcg gttccagcaa tgaatcgcgc aacaccagct
    13261 caaggttctg ctcatcacgc ttgataagct ggtgcagagc aatctggttg atatccagac
    13321 tcatgataaa ctctccttta agaccgggcg gtattcaacc accgccggtt aagcgacgca
    13381 ataaaagatg gcggtcattt tacagaaggc ggcgtaacga gaatgattta acgcataaaa
    13441 aagcagaaaa aaaaccgttg ctacggtaat atgttgccct ttcatcaact aactgatttc
    13501 gatttatgcc acaaatttcc cgctacagtg atgaacaggt tgaacaattg ctcgcagagc
    13561 tgctcaacgt actggaaaaa cataaggctc cgaccgacct ttccctgatg gtgttaggca
    13621 acatggtcac taaccttatc aacaccagca ttgccccggc ccaacgccag gcaattgcca
    13681 actcttttgc ccgcgcctta cagtcctcta tcaacgaaga caaagcgcac taagggaaac
    13741 agataacagg ttatggtaac tcatcgtcag cgctaccgtg aaaaagtctc ccagatggtc
    13801 agttgggggc actggtttgc actgttcaat attctgcttt cgctcgtcat tggcagccgt
    13861 tacctgttta tcgccgactg gccgacaacg cttgctggtc gcatttattc ctacgtaagc
    13921 attatcggcc atttcagctt cctggtgttc gccacctact tgctgatcct cttcccgctg
    13981 acctttatcg tcggctccca gaggctgatg aggtttttgt ccgtcattct ggcaacggcg
    14041 ggaatgacgc tattactgat cgatagcgaa gtctttactc gtttccatct ccatcttaat
    14101 cccatcgtct ggcaactggt tatcaaccca gacgaaaatg agatggcgcg cgactggcag
    14161 ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac tggtgtttgc gacgtggagc
    14221 tggcaaaagc tacgcagcct gacgcgtcgt cggcgcttcg cgcgcccgct ggccgcattc
    14281 ttatttatcg cctttatcgc ctcgcatgtg gtttatatct gggccgatgc caacttctat
    14341 cgcccgatca ccatgcaacg cgctaacctg ccgctttcgt acccgatgac ggcgcgacgt
    14401 tttcttgaga agcatggtct gcttgatgcg caggagtatc aacgccgtct cattgagcaa
    14461 ggtaatccag acgccgtttc cgttcagtat ccgttaagcg aactgcgcta tcgcgatatg
    14521 ggcaccgggc agaatgtgct gttgattact gtcgatggcc tgaactactc acgcttcgag
    14581 aagcagatgc ctgcgctggc aggttttgct gagcaaaata tttcgttcac gcgccatatg
    14641 agctccggca acactacaga caacggcatc tttggcctgt tctatggcat ctcgccgagc
    14701 tatatggatg gcattctgtc gacccgtacg cctgcggcat taattactgc gcttaatcag
    14761 caaggctatc agctggggtt attctcgtca gatggcttta ccagcccgct gtatcggcag
    14821 gcattgttgt cagatttctc gatgccgagt gtacgcaccc aatccgacga acagaccgcc
    14881 acgcagtgga tcaactggct gggccgctac gcacaagaag ataaccgctg gttctcgtgg
    14941 gtctctttca atggcacaaa cattgacgac agcaatcagc aggcatttgc acggaaatat
    15001 agccgggcgg caggcaatgt cgatgaccag atcaaccgcg tgctcaatgc actgcgtgat
    15061 tctggcaaac tggacaatac ggtggtgatt atcactgccg ggcggggtat tccgctgagc
    15121 gaagaggaag aaacctttga ctggtcccac ggtcatctgc aggtgccatt agtgattcac
    15181 tggccaggca cgccggcgca gcgtattaat gcgctgactg atcataccga tctgatgacg
    15241 acgctgatgc aacgcctgct acatgtcagc acacctgcca gcgaatattc gcaaggtcag
    15301 gatttgttca accctcaacg ccgccattac tgggttaccg ccgcggataa cgatacgctg
    15361 gcaattacca ccccgaaaaa gacactggtg ctgaacaata acggtaaata ccgcacttac
    15421 aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt taagtttgtt attgcaagta
    15481 ctgacagacg agaagcgttt tatcgctaac tgattaatta taaatcagtt agcaaaatat
    15541 cttacttgca atcggtgtgg aaaacggtag tattagcagc cacgagtcgg cacgtagcgc
    15601 agcctggtag cgcaccgtca tggggtgtcg ggggtcggag gttcaaatcc tctcgtgccg
    15661 accaaaaatc ccaagaaaac caacctattg cggttggttt ttttatatct gcatttaatt
    15721 cgataaacag acagcgacac atcacgacct gtttattttc tgttatcaga acgtccagac
    15781 tacacccgcc tgagttgtgc tgctttcttc acgggagaaa cgactatcga taccgagcgt
    15841 caggcccgtc aatgtttgct caaagccagc tcccgcatca gtgttcacgt tgttgcggct
    15901 gttaattgcc gaactccaca tcgccgccct ttacgctacc aagacgctca cgcacggtgt
    15961 ccagttctgc atcaaatacc agcggttgtg ctgccgccat attcagcaca tccgtggttg
    16021 aagggggcat ttgtacgcga ttctctgtga gactccagct atggttgcca ttatccagca
    16081 aggtatattc atacgtactg atatcaacaa cgcttccggc attgcccaac gtaaatgccg
    16141 catcaccgcg ccctccttta atcgtgggat cagtcgccgc gccataaacc tgtagcactc
    16201 cattattgat cgtagtctgg tttaccgcgc cacccatttg aacaatttga taaccatcat
    16261 ttaatactga gtattctgcg gtgccattta tttcaatgta ctgatatccc ccgttaattt
    16321 tagtattact acttacacca aattcttata tatgctgttc tccgccagga ttgattttcg
    16381 tattattggc ggccccataa accagctgga tatctttttc caggacaaca ccatcaactg
    16441 tctcaccata ctcaacagtt gatgccaatg ctatgggtga atataacgct gtagccacca
    16501 gaacagatat tgcggaacga caaagagaaa cagaaccaga ttggtgcatt aagctttcat
    16561 cctatgaaat taattgctgt taaaagcatt ggggacagaa aatacccata gctccatacc
    16621 cggagtcagt tttaaaaaac tgtttaaaga aatgcacaag tattgtgatt gattttttag
    16681 ttgtttttcc tgatgagaag ctgatgcaaa attccgtctt tataatgaag atgatgccaa
    16741 agcgaacgac aaagttgtag ttttcactac atgtccatac ataaaatggg gtaacattca
    16801 cgcgcctggt agcgttacca acgctacgct caaacataat gattctaata aaacctcagg
    16861 agactactat gcctgaagca acgccttttc aggtgatgat tgtggatgat catccactta
    16921 tgcgacgcgg tgttcgtcag ttactggagc ttgatcctgg ctttgaagtg gtcgccgaag
    16981 cgggcgacgg cgcgagcgcg atcgatctgg cgaatagact ggatatcgac gtgatcttgc
    17041 tggatctcaa tatgaaaggt atgagtggcc tggataccct caatgccttg cgcagggatg
    17101 gcgttaccgc gcaaattatt atcctgaccg tatccgatgc ctccagcgat gtctttgcgc
    17161 tgatagacgc aggcgcagac ggttatctgt tgaaagacag cgacccggaa gtgttgcttg
    17221 aagcgattcg tgccggagcg aaaggcagca aagtttttag cgaacgcgtc aatcagtact
    17281 tacgtgaacg tgaaatgttt ggcgcggaag aagatccctt cagcgtgctg acggagcgcg
    17341 agctggatgt tctgcacgag ctggcacagg ggctgtcaaa taaacagatt gcctcggtgt
    17401 tgaatatttc cgagcagaca gtaaaagtac acattcgcaa tctgctgcgt aaactcaatg
    17461 tccgctcacg cgtggcggcc accattctgt tcctgcaaca acgcggggca caataaaaat
    17521 agcccgatgg catcaccatc gggccacttt ttatttactc tcctgcggcg acaaatgttg
    17581 catcgcctgc gcgatactac gttcgatcac cgcacggcga gtatcgttgg caggtaagag
    17641 tttcaacatc atttcccacg cggcaacggc ttcgccaaat cgttgctgct caaaggcatt
    17701 aaatgcatac atgcttaaca cacggatatt gctatggtcc gttctcacca actggcgcag
    17761 cagttcgccg ccgaggcggt tgtcgttggg atcagatgaa cgtgtcaacg cttcagcgta
    17821 tccgagcgcc gcatcactgt tcttcggatc gaggcgatat gcagtagcgt atgcatcggt
    17881 ggcgatactg gcgttaccca gcgccatgcc aacgcggccc aacataatcc agccttctat
    17941 atctcccgga tttttttgca gttgagtacg catccccagc gcaaggcgcg acatctcttc
    18001 ttcgttgagc ggagcggctt tcggatccag cgccctgtcc agtaacgccg gagcctgcgc
    18061 ggtggcctgc tgccagattt tcacctgctg ataattgcca gtctggtagt agctgacgcc
    18121 agccacaatt aacgccacca caatacccgg cagataaaca acatatccgg cacgcttacc
    18181 ttccggcacg ctttgttctg gaaacgcttc cggcaccacg cgtacccgac gccgcgaacg
    18241 ggcgtatatg acccaaccgc caatgccaat agccactact ggcagcaccc acagcagcac
    18301 ggtcagcggc gttaacggcg gatcgtaagt gacgaagttg ccgtaacgcg ccaccatata
    18361 atcgacaatc tctttcttac ttttgccttc ctgcatcagt tcatacactt tctggcgcag
    18421 gtcggtggca atcatcgagt tggaatcggc aatgctgttg ttctggcatt tcgggcagcg
    18481 cagttcttca gtgagctgac ggaactgttg ttcctgtgct tcatctttaa actgcaacac
    18541 gtcgatggtc gccagcgctg agccggagat catcagcatc agcacgccca ataaaaacct
    18601 cattgtgcgg cctccttact gtatttctcc cacagtggct tgatctcttc ttcccagacg
    18661 cgaggattga gatcgcccgc atggcgatag cgaatgatgc cgttgccgtc aataaggaac
    18721 gtttctggcg cgccatagac accgagatcc agccctaaca tgccgtcgcc atcaaacagg
    18781 cttagtgcgt aaggattgcc caactctttc agccagctga ttgccttctg gcgatcgtct
    18841 ttatagttca tgccgaccac gcggatgccc tgcgcagaaa gctgattcag atattgatgt
    18901 tccgcacggc aggtcggaca ccaggtcgcc cagacgttaa gcagcaccgg tttgccctga
    18961 gtcagcacat ccgcctgata aaactgcccc ggattgtcca gtgattcgag gcgaaacttc
    19021 ggcactagct tgccaatgag cgccgattcc agattggtcg gatcatcccc ttcggcatta
    19081 cgcgccagct gccacagtag cgccgcggca atcgccagga agataatcaa cggaattaac
    19141 aatactttgc gcttcataca gcctccggcg cagttttctg cgggttcacg cgcttacgat
    19201 agcgaggatc aaacaggcac agcagcc
//
LOCUS       DAZKJR010000099        19110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKJR010000099 DAZKJR010000000
VERSION     DAZKJR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.100"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            206..1000
                     /gene="hyxR"
                     /locus_tag="ACHOWS_003507"
     CDS             206..1000
                     /gene="hyxR"
                     /locus_tag="ACHOWS_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFQ0049032.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            1154..1915
                     /gene="hyxA"
                     /locus_tag="ACHOWS_003508"
     CDS             1154..1915
                     /gene="hyxA"
                     /locus_tag="ACHOWS_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFQ0049033.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVANAGTQALSLHKYK"
     gene            1957..4535
                     /locus_tag="ACHOWS_003509"
                     /pseudo
     CDS             1957..4535
                     /locus_tag="ACHOWS_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            4717..5385
                     /gene="ykgH"
                     /locus_tag="ACHOWS_003510"
     CDS             4717..5385
                     /gene="ykgH"
                     /locus_tag="ACHOWS_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFQ0049034.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTRPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKSKYPAAICLILGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(5626..6321)
                     /locus_tag="ACHOWS_003511"
     CDS             complement(5626..6321)
                     /locus_tag="ACHOWS_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFQ0049035.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERFTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(6314..7741)
                     /locus_tag="ACHOWS_003512"
     CDS             complement(6314..7741)
                     /locus_tag="ACHOWS_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFQ0049036.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7752..8471)
                     /locus_tag="ACHOWS_003513"
     CDS             complement(7752..8471)
                     /locus_tag="ACHOWS_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFQ0049037.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGLKVKVMHIAEVLMSR"
     gene            complement(8999..9853)
                     /gene="rclR"
                     /locus_tag="ACHOWS_003514"
     CDS             complement(8999..9853)
                     /gene="rclR"
                     /locus_tag="ACHOWS_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFQ0049038.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            10079..11404
                     /gene="rclA"
                     /locus_tag="ACHOWS_003515"
     CDS             10079..11404
                     /gene="rclA"
                     /locus_tag="ACHOWS_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFQ0049039.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADIPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            11513..11749
                     /gene="rclB"
                     /locus_tag="ACHOWS_003516"
     CDS             11513..11749
                     /gene="rclB"
                     /locus_tag="ACHOWS_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFQ0049040.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            11761..12354
                     /gene="rclC"
                     /locus_tag="ACHOWS_003517"
     CDS             11761..12354
                     /gene="rclC"
                     /locus_tag="ACHOWS_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFQ0049041.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIIFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(12514..13383)
                     /locus_tag="ACHOWS_003518"
     CDS             complement(12514..13383)
                     /locus_tag="ACHOWS_003518"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0049042.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            13632..14489
                     /locus_tag="ACHOWS_003519"
     CDS             13632..14489
                     /locus_tag="ACHOWS_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0049043.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKSFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARMNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(14614..18864)
                     /gene="fdeC"
                     /locus_tag="ACHOWS_003520"
     CDS             complement(14614..18864)
                     /gene="fdeC"
                     /locus_tag="ACHOWS_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFQ0049044.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGITVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEALVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPAKLVLDPNGTATTNEQGQA
                     IFTATTTVAATYTLTAQVSQNNGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
BASE COUNT         5057 a         4615 c         4315 g         5123 t
ORIGIN      
        1 cccatgattt gaaatgatca accacaaagg tcataccagt gatcagtttt cactcgcgtt
       61 gttaaataac acaaaattaa aaacaataaa aatcaaacaa attagattta tattccaata
      121 aaattaaata ttcaggcaat taaaccaaac ctgacatttt gaatcaaaat gtcaggtttt
      181 gtagcaatgg cgtggacgag tctgaatgaa gaagcctctg gttattatca gtgcctgtca
      241 gttcaccagg ctggcactgg agtctctcat cccaacggac aggtatatcg tgcgggtgta
      301 cagcaacgtc actacagagg ttgaattcgt tttaaagacc tcctgtggct acctgttagc
      361 tgatatccca tcgcttcctt cgggagagtt ggttcttctg gcttgtcttg tccgcttgcg
      421 gagtgatgcc gggcaatggc aggtctgcct gaccggtgac tccgactggt tcgataatgc
      481 tcctgctgca agtctgtata acggcttccc acgcatcggc accatgctga cagcgagtcg
      541 ttatcagaat caaattatcc gctggttact acgtcctttg cccggcgata tgagcgattt
      601 ctggctcctg actgcacggg agttgagcgt gcttaagatt ctgatgcagg gtatgagttt
      661 cagtgagatt gcccgttatg aacaacgcag ttcaaagacc ctgcatgcga ttgccacacg
      721 ggctttaatg aaacttggac tgggaacact gagcgatttc aggctgctct atacgggatg
      781 cggtgacagc agagtgaaac ggaatatacg gctacatgca aaatatatcg gacaacattc
      841 cgctcatcga ctcactcagt atgtgcagaa catggtatcc ggaattcttt atccagagaa
      901 agttgccgtt ggtgaatcaa taaattctga cgccatatcc ggaaaagtac tttccggaca
      961 aagaaaaaat atgtctaaaa aaagaccagg attgttatga cacagcagcg ttgttactac
     1021 cccccactgc ggcgcatacg gactcgctac agtgtaggag ccaaaatatt tctactgctc
     1081 tgggctgcag gattgggagg cacgctgtac agcctgtaca gcgtgctggc gtactgacat
     1141 ctgtgaggga aggatgcgtt tcgttctttt ctgtccctca ggaattgtac cagcgcagtt
     1201 cgcagctcta tcaaccggtt ctgtcggcaa tgttacctgc atcagaacag aagaggagtt
     1261 acgcaataaa ttgcggcaca ggccacagag tgtcgtgata tcggcagggc gacctgccga
     1321 atgcgctgaa atgtggttcc gattttatcg ggatcactca ttcgtggtgg tgttgtgcgt
     1381 cgcacccttt tttctgccac cagatgtgag tatttctggc gttctcaaaa acctgaggtt
     1441 actgaaacca ggaatgtcag tagagcgtgt catcagtatt gcaaatgcct ctggtgggtt
     1501 ttcagggctg aaacatgcag aaattttgcc agttatggac agttactctg tctttatgaa
     1561 agaggtgaat aaccgcacaa agacaatcgt catgtcagaa cgcttcccgg aaaagcaaaa
     1621 gaaggtgttg tcgttgcttc ttgccggaca cagctgggaa tacagtgcgc agtttctcaa
     1681 aacggggata agacagatat ggcttgccga gcagtcgcta aaaaagcgtt ggggaatacc
     1741 ggacagcatg tcattgcggg aagccctgct gctgagcagc aataattttc acggtgatgg
     1801 aaatgcattg gaaacaacga atgccatgac attgcgtgaa aacggaaata ccaacaggta
     1861 ttccgtggtg gcaaatgcag ggacgcaggc tttatctctg cataaatata aataagtggg
     1921 aaattgaaat tagttaccag aatggagaat ttcttcatga aaaacagtaa ggcattttac
     1981 cgcagcgcat tagcaacagc tattgttatg gctctttctg caccagcatt cgctgctgat
     2041 aaagcggtat caactgattc ggttacgctg aataaagata agacaactct ggatcaagat
     2101 gttgttatta acggtgataa caagattaca gccgtaacaa ttgaaacgtc agattcagat
     2161 aaagacctta atgttacttt tggcggtcac gatattaccg ccgcatcaac ggtaaaccaa
     2221 ggtttcgttg aaggtgtaaa agttagcggt gacaaaaatg ttgtgattaa tgctacaggc
     2281 tccaccatca cagctcaagg tgaaggcacc tatgtccggt ctgcaatggt catttcttca
     2341 actggcgatg ttgttgttaa tggcggtaat ttcgttgcaa aaaatgaaaa aggtagtgcg
     2401 acagggatat ctctggaagg ggcccaggga aataatgtca cgcttaatgg cacaaccata
     2461 aatgctcaag gtaataagag ttccagcaac gcctccacgg caatttttgc tcaaaagggt
     2521 agcgtattga atggttttaa cggtgatgca acagacaaca ttacccttgc tggctcaaat
     2581 attattaatg gtcggattga aacaatactt attgctcagg agaataaggg aactcataca
     2641 gtcaacctga agattaagga tggctcagta attggggcgg ctaataataa acaatttatg
     2701 cctcagcttc ggcacaagga aaaggttcag caacgcaaaa tttaaatttg tctgttgctg
     2761 attcaaccat ctactctgat gtcctggccc tttctgaaag cgagaattca gccgctacca
     2821 caacaaatgt aaatatgaac gttgcccgct cttactggga aggtaatgct tatgcttatg
     2881 cttatgctta tgcttatgct ggtgtttatg ctgatgacga taaagcgagt agtaatctga
     2941 atataaatct ttccgatggc tcagtctgga aaggaaatgt tgcgggtagt acgactactg
     3001 gcagcaccgg tactacagaa aaccagtcag caaccgtaag tctgtctgat tccacctgga
     3061 aaggtaacgt gggtgacggt ctggatggtg gtgcgcgagt cacgctgaaa aactccagct
     3121 ggactggtga tgtcggcaac gccaaaactg ctgtttttct gacggatggt tctgtgtgga
     3181 atggtgctgt ttccattaac gcgccagtgg accgcgcatc agtaccaatg ggctcggttc
     3241 aaccaaataa aagcagtgta tctctgcaaa acgggtctgt ctggaatgtt acgggagcct
     3301 caactgttga tgctctggca gtaaaagaca gtacggttaa tatcacgaag gctacagtca
     3361 atactggcac gtttgcttct caaaacggca ctctgattgt tgatgcctct tctgaaaaca
     3421 ccctggatat cagcggtaaa gcgagcggtg acttgcgtgt ttacagtgcg ggttcattgg
     3481 atcttatcaa tgaacaaacg gcatttattt ctaccggcaa agacagcact ctaaaagcca
     3541 caggcacaac ggaaggtggt ctgtatcaat atgacctgac acagggagct gatggtaact
     3601 tttatttcgt aaaaaacacg cataaagcat ccaacgccag ctccgtgatt caggcaatgg
     3661 cagctgctcc ggctaacgtc gctaatctgc aggccgacac gctctccgcc cgtcaggatg
     3721 ctgtccgtct gagcgaaaat gacgagggtg gcgtatggat tcagtacttt ggcggtaaac
     3781 agaaacatac caccgcagga aatgcatcct atgacctgga tgtaaacggt gtaatgctgg
     3841 gtggtgatac ccgcttcatg actgaagatg gtagctggct ggccggtgtg gcgatgtctt
     3901 ctgcgaaagg tgacatgacc accatgcaga gcaaaggtga cactgaaggt tacagcttcc
     3961 acgcttacct gagccgccag tataacaacg gtatcttcat tgatactgct gcacagtttg
     4021 gtcattacag caacacggca gatgttcgcc tgatgaatgg tggcggtacc atcaaagctg
     4081 actttaacac caatggtttt ggtgcgatgg ttaaaggcgg ttacacatgg aaagacggta
     4141 atggcctgtt tattcagcca tatgccaaac tgtctgctct gacgctggaa ggtgtggatt
     4201 atcaactcaa cggcgtggac gttcattctg acagctataa ctctgtgctg ggtgaggccg
     4261 gtacgcgcgt gggttatgac ttcgctgtgg gcaactcgac cgttaaacct tatctgaatc
     4321 tggccgcact gaacgaattc tctgatggca acaaagtccg tctgggtgat gagtctgtca
     4381 atgccagcat tgacggtgca gcattccgcg tgggtgcagg tgtacaggct gatatcacca
     4441 aaaacatggg agcatatgca agccttgact acaccaaagg tgacgacatt gagaatccgc
     4501 tacagggtgt agttggtatc aatgtgacct ggtaagaata aagcagtcgg ttcccctgtc
     4561 gactgttata ccccctgcag gcagcagtat gctgcctgca ggctataaat atccagtatt
     4621 catgatgctg gatatttgct gatatttaac ccgaacattc ctgatacaga ctattaattc
     4681 atttatctgc ggcagatttt attttctgaa tatattatga atgaacaaat caaacaggat
     4741 atcgatctga ttgagatttt attttatctg aagaaaaaga ttcgtgttat cctttttatt
     4801 atagctatct gtatggcaat ggtgctgttg tttctgtata tcaataaaga taatataaaa
     4861 gtgagttaca gtctaaaaat aaaccagaca acaccaggca tacttgttag ctgtgatagc
     4921 aataataatt ttgcctgtca gaccacaatg actgaagatg ttattcagcg aattactaca
     4981 ttttttcaga ccagaccaga tgtcaagaac agagaaataa agctggaatg gtcaggagat
     5041 aagagagatt taccaactgc tgaagcggaa atatctcgcg tgcaggcgtc tattatcaaa
     5101 tggtatgcgt cagaatacca taatggcagg caagttcttg atgagataca aacgccttca
     5161 gcaattaaca gtgagcttta tacaaaaatg atatacctga ccaggaactg gtcattgtat
     5221 ccgaacggtg atggctgtgt aaccataagc tcaccggaaa taaaaagtaa ataccctgct
     5281 gccatttgtc tgattctggg attttttcta agcattgtga tttctgtaat gttttgcctt
     5341 gtcaaaaaaa tggtagatga ataccaacaa aactctgggc agtaataaac agcattctga
     5401 cagagagtgc ctgtataaaa ctatagtgac aatgaccggg aagggagccc gtcttcagga
     5461 atggattcct gtctttttca ctgtaaatat cctggcatta ttaatgtcag gatatttaca
     5521 gaagagcagc ataaccatga cggaacggag ttaagtcagg ttgtcattag cgctattcgc
     5581 aacctcgtgc cgccttcagg tttatgtcca gacttcatat ctctctcagc aatcctcaat
     5641 aatcagatac accgctttca ccggaccatg aaccccgacg actttgataa gctcaatgtc
     5701 cgccgttgaa ctgggaccac tgatgatgtt aatacatgac ggcattcgtt caccggcctg
     5761 cgctttctga tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg
     5821 caggataaaa agagaagatt ccgggagcag gctcaatgaa cgcccgcgcc cggcggcgga
     5881 aaaaagaacc acgcctcccg attcggttaa accatattca gcatatacga cgcccacttt
     5941 agcctgctct gcttgtgata tgttctcaat acctttcgcc ggatcccaaa caacggcatt
     6001 gcattcctgc tgcaaacgtt cgctaatccc caattcctcc aacctcgtgt caccactaat
     6061 cacgaccgac tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc
     6121 ctcgctggtc agttcacagc gcgtcaacat aacatcgctg gcaaactgga taaacgcgtc
     6181 acagcgctgc tgttgggtaa gttgggtaaa ccgctcgtta gcatagttgt taagcggcgc
     6241 atcttctgct tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa
     6301 aaattcgctc cgattatcca ttctttttct cctgcgcctg atgtttctta aaccaactac
     6361 ggaaactctc tccgtcagct tcaggaagat cgcgtgcttc catccagtcg ctaatcgcgc
     6421 caaatttgag tggtgttttg ccgccattga taaaccagct tgccgcatga gcaccagcca
     6481 tcatcccaac tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc
     6541 gttgctctgc ttttgcggtg atcccttttt cagccatcac ccgacgatga cgcaaaatca
     6601 gttttgacag cggaatacgc accggacaca cgctgtcaca agcggtgcat aaagagcagg
     6661 cgtagggtaa atctttaaaa tctttatagc cgccaagtaa cggggaaatc accgcaccaa
     6721 ttggccctgg ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag
     6781 tattcataca agccccgcaa cgaatacagc gcagcacatc ccgaaattca gaggccagca
     6841 cctcagaacg cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc
     6901 ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac
     6961 tgcgcgccag catggtgatc aatacatcta cctctgcaaa cgtgggagca atacgctcca
     7021 ttcccatcac tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat
     7081 tggtcaccag gcataccgaa ccggtctctg ccaccgcgaa attacagccg gtaataccta
     7141 tttcagcact gaggaaatct tcgcggattt tttgccgaat gaataaggtc atcgcttcag
     7201 gcgtttccgg cccctcatag cccagacgtt cgtgtagcac ccgacggatc tgatggcgat
     7261 ctttatgaat tgccgggacc acaacatgag agggcggatc ttgatccagc tggagaatgt
     7321 attcacccag atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac
     7381 caatctcttc ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg
     7441 caacctgtaa aatgtagcgg gtagcgtctt ctttggtttt tgcaaaatag acgtgaccgc
     7501 cgttttgcgt cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat
     7561 gatcacgtat ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca
     7621 ttttttgccg atttgccccg atacgctgct gcgcgtttgc caccgctttg cgcatgatcg
     7681 gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca
     7741 tctttatatc ctcagcggct catcaacact tcagcaatat gcatcacttt gactttcagc
     7801 ccttcccgtt gtaatcgtcc actgatgttc agcaggcaac tcacgtcagc acctattaaa
     7861 tactcagggc ggacttccat caggtgcgca accttttctt tcaccatctc gccggatatt
     7921 tcggccattt tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag
     7981 gtcaacagct ccagtccacg cacatttttc agcagcgtaa gtggttcgtc cttcaccccc
     8041 agcttacggg ccaggctaca agatgggtga tacaccgctc tcccttgcaa actggcacct
     8101 acatcgacta cccctaatgt attaacaata aaagaggtga gatcctgcat acgcgcggca
     8161 accttttcgg cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt
     8221 acggcatagg tgcaagagcc agctggtgaa ataatgggat cgtcgttatc ctccagtgcg
     8281 gcgatcagat ttttcatccc tggaatcgct tctttgatat aaccactatt gatcgcaggc
     8341 tgaccgcagc atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc
     8401 agcacggagt ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa
     8461 ttgacattca caactaatac tccattactt catgcccatt tatgcccgat ggttcccccc
     8521 ttccggggga tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc
     8581 acccctcatt tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat
     8641 tttatttctt tcggcatcgc tcacatttta tagggaaatc gacgatcatt tttggcataa
     8701 gaaatccatt taaaaaggct acatccctta tacctattca gtgctactta ttcaggggtt
     8761 attgagatat acactgatgg tgtacaaaca aaatcaccaa caataattac gtatcaagta
     8821 atagtcggca ttttatttaa ttaaagcaat ttagtttgcg tttttattta aaaactgagc
     8881 tagagtccat caaaaaaaat atataaagcc caaaaccacc gttggaaata gaaatagttg
     8941 aaaggctcac aactcccgga atgcggatta tctttaccct gatttctggc ggttttattc
     9001 agggtgcaag ctgtctcacc ctttcccgat attctcccgg agtacaacca aactcgcgga
     9061 caaacgcctt gtgaaaagat gattcactgg catagcctac tgactcagcg atcaccacaa
     9121 cagggagcat ttcccgggaa aacatctggg ccgctatttg tagacgcaac tttgttaata
     9181 cagccagcgg cgtggttccg gaaacatcac ggaaaagctg ggcaaaactt gcccgggaca
     9241 tgtgggcgat gctggccagc gattcgacgg tccaggcgtg tcctggcatt tccagcattt
     9301 gctgtattac cgcaccaaga cgtggatgca gaagcaaact gagaatgttt ttctctgtat
     9361 taacctgtgc aatccactca cgcaccgcga gggtaaagaa tgtagcgcag atctggctac
     9421 acagagcatc cactcccggc attgccgatc tggattcctg ttgtaaaaac gggatcgcct
     9481 ccctcagcca gttatattcc acgctatggt tcactggtgc tacaaacagc gtttccggca
     9541 aagatgttaa aaaataacgc gccgaatgtt gcagccgaag agtgccacag acaatacagg
     9601 tcgattcatt atcgacatga ctcaggcgat gagctgaatt ttgtggtagc aggaccacat
     9661 ttcccgggcg taatgtaaaa atctcccccg tcggcatttc cagcttcgcc gctccttgcg
     9721 ttaacgcatg ccaacgaata accgacaatt ccccggcacc atgtggaagt tgccagtcac
     9781 ttcctaacac gcaattctta tcgatcgttc cttgtggagc gttaagcatc aacaaacggc
     9841 taagggcatc catatcagac tcctgagtgg tttttgatta caaacaccaa caatcttaga
     9901 caataattaa tctaatatca tcaaatggtt aatcatgatt tttgttttgt gattctgatt
     9961 aagaaaaaga aaataccaga cgataagaca aaatatcaag cgttgtgaag aaatgttatt
    10021 tgctcttttg cgtctatagt catgatgtca aatgaacgcg tttcgacagg aaatcatcat
    10081 gaataaatat caggcagtta ttattggttt tggcaaggct ggaaaaacat tagccgtcac
    10141 gctggcaaaa gcaggttggc gtgtggctct catcgaacaa tcaaatgcaa tgtatggcgg
    10201 gacctgtatt aatatcggct gtatcccaac caaaacattg gttcatgacg cacagcagca
    10261 cacagatttt gtccgtgcca tacagcgtaa aaatgaagtg gttaattttt tacgtaataa
    10321 gaattttcat aatcttgcgg atatacccaa tatcgacgtg atcgacggcc aggcggagtt
    10381 tatcaataat catagcctgc gtgttcatcg gcctggggga aatctggaga ttcatggcga
    10441 gaaaattttt attaataccg gtgcacaagc cgtggttccg ccaattcctg gaattaccac
    10501 cacgcctggt gtatatgaca gcaccggatt acttaatcta aaagaattgc ctgggcattt
    10561 aggtattttg ggcggcggat atattggcgt tgagttcgcc tctatgttcg ctaattttgg
    10621 cagcaaagta accattttag aggcagcttc gctgtttttg cctcgggaag atcgggatat
    10681 tgctgataat atcgcgacga ttttacgcga tcagggtgtc gatataatcc tcaatgccca
    10741 tgtggagcga atcagtcacc atgaaaatca agtgcaagtg catagcgagc acgcccaact
    10801 ggcggtggat gcactgttaa tagcttccgg tcgtcaacca gctaccgctt cgttacatcc
    10861 agaaaatgcc ggtatcgcag taaacgagcg cggggcaatt gtcgttgaca agcgattaca
    10921 taccaccgca gacaatattt gggcgatggg agatgttacc ggcgggctgc aatttactta
    10981 catatcacta gatgattacc gcattgtacg tgatgagtta ctgggtgaag gcaaacgtag
    11041 tactgatgat cggaaaaatg tgccttattc cgtatttatg acaccgcccc tgtccagggt
    11101 tggtatgaca gaagaacaag ccagagagag tggtgctgat attcaggtgg tgacattgcc
    11161 tgtagctgca attccgcgcg ccagagtgat gaacgatacc cgtggggtat taaaagcgat
    11221 tgttgataat aaaacccaac gtatattagg ggcatcactg ctatgtgttg actcccacga
    11281 gatgatcaat atagtgaaaa tggtgatgga tgccgggctg ccttatagca tattacgcga
    11341 tcagatattt actcatccgt cgatgagcga atcactcaat gatctatttt cattagtcaa
    11401 ataaactcaa aatcagacgc cagaacaaat atcctggcgt ctcagagaaa agaatcttat
    11461 taattctctg ttactctata aattcttaat cacttcattg atggtatttt atatgtttaa
    11521 aaaatctgtt ttatttgcaa cactattatc tggcgttatg gcattttcca ccaatgcaga
    11581 tgataaaata attctgaaac atatcagcgt ctcatcagta tcagcatcac cgacagttct
    11641 tgagaatgcc attgctgata tggccagaaa atataatgct tcatcctgga aagttacatc
    11701 gatgcgaatt gataataatt caaccgcaac agcagtattg tataaataag gatgtttaca
    11761 atggaaagat acctgcacct gttaagtcgg ggagataaaa ttggcctgac attgattcgt
    11821 ctgagtattg cgattatttt tatgtggatt gggttattaa agtttgtccc ttacgaggca
    11881 gacagcatta caccattcgt cgcaaacagt ccactaatgt cgttctttta tgaacacccg
    11941 gaagactata aacagcatct gactcacgaa ggcgaataca aaccagaagc aagagcatgg
    12001 caaacggcta ataataccta tggtttttcc aacggtcttg gcgtcgtgga ggtgattatt
    12061 gctctgctgg ttttggctaa tcctgtcaat cgctggttag gtttattggg tgggctgatg
    12121 gcatttacca cgccgctggt aacactctca tttttaatca ccaccccgga ggcatgggta
    12181 cccgcattgg gtgacgctca tcatggtttc ccttatttat ccggtgctgg tcgtctggta
    12241 ttgaaagata ctctgatgct ggcaggtgct gtcatgataa tggcagattc ggcgcgggat
    12301 attcttaaac aacgcagtaa tgaatccagt tcaacgttaa aaactgaata ttgataatcc
    12361 gaacactgtc tgttgttcac tttttcaggc gtcaggcccc ctttctgatg atgaagcctg
    12421 acgccatatt accggagata tgacagagtg aagatcaata tcactcccct gttaaaagat
    12481 tcttgtcgtc tcaggtaaaa taagtgatta atatcaatca tgtattttta cagcaagaca
    12541 accacgaaca aactcaggat taaaatgttt taccgattcc ccaacataac ccaaatcaag
    12601 cgaactaatt tgcgccattt ctttctctgt cagtgagaaa tcccagatag caaaattttc
    12661 ttcgatacgc tgttgatgtg tcgatttcgg aataacgacc actccccgtt gaatattcca
    12721 acgcagaatg acctgagaaa tcgatttttg atgggcatct gcaatactct gaagcagatt
    12781 attttcaaag ggtttatgtc gtccaccacc taacggagcc catgcttcag gctgcacgtt
    12841 ataatacttc atggttgcca gcgcctctgg ttgggcaaaa tagggatgca actcgacctg
    12901 gttgaccatc ggtttaactc ttaccgtttc acaaaagttc gccagaacat gagggtagaa
    12961 attggaaacc ccaatcgctt ttaatttgcc ctcttcatag gcatcttcga gtgcacgcca
    13021 cgcgctaaaa taatcgcgca tggcctggtg caataaatag agatcgaagt attctagtcc
    13081 cgattttttt aatgatgctt caatacctgc tgctgcaata tcctgattca acatatcctg
    13141 cacccacaat ttagacgtta taaataactc ttctcgggta cataaacctt cagatatagc
    13201 ttcacgaacg gcttcaccga cggcatcttc attaccgtat accgctgcag tgtcgatgag
    13261 tcgataaccc gtgcggatag cgttcaacac tgactgtttg cacacgtttt tatcggtaac
    13321 ctgaaaaaca ccaaatccca tcatcggcat tttcagattg ttacttaata cggaaaattc
    13381 cactgtcagt ccctccatta ggcatgaaca atgagtctac gttaaaacgt aacctcaaat
    13441 tagtatgtgg attttgatat cacttatgca aaaaattcat taataatgta ggactgaaac
    13501 ctctctattt tcggggacaa cgaagcagac gctaccagtg cttttgcctt cgcccttgct
    13561 gtttttgata cacttagggc ccagggcata acgaaaatgt gcgatatgac aaagaattaa
    13621 cggagaatgt gatgatcagg cagaagattc tacagcagct cctggagtgg attgagtgca
    13681 atctcgaaca ccctatttca atcgaagata tcgcacagaa atctggctac agcagacgca
    13741 acatccagct tctgttccga aatttcatgc atgtgccttt gggagaatac attcgcaaac
    13801 gaaggctttg tcgtgccgcc attcttgtcc ggctctccgc gaaatctatg cttgatattg
    13861 cactctcttt gcattttgac tcacagcagt cattcagccg cgaatttaaa aagttattcg
    13921 gctgctctcc ccgtgaatac cgccaccgtg attattggga tctcgcaaat atcttccctt
    13981 cttttttaat acgtcaacag caaaagacgg agtgtagatt agtcaacttt cctgagacac
    14041 ctatttttgg taattcattt aaatatgata ttgaagtgtc gaataaatca ccggatgaag
    14101 aagtcaaact gcgacgtcat catttagcca gatgtatgaa gagttttaag acggatatct
    14161 atttcgtctc cacgttcgaa ccatcaacaa aatcggtcga tttgctcaca gttgaaactt
    14221 ttgccggtac ggtatgcaaa caaaccgatt cagagatgtc gaaagagtgg acaattaacc
    14281 gaggactgta tgcctctttc cgctatgaag gtgagtggga gcactaccct gaatgggcgc
    14341 gaaacctcta tctgatggaa ttacctgcca gggggttagc cagaatgaac ggcagtgata
    14401 ttgagcgatt ttattacaat gaaaacttcg tcgaaattga tagcaataac attgtttgcg
    14461 aaatttttat ccccgttcgc ccggtttagc ctgtaactcc atcacataag atttacccta
    14521 ccctgccacc gtctaatcgg tgccagggta ggtattcagt aaataatggc gctgcgcatg
    14581 tcaacgccat tattatttat tacatcgtcg tttttatttc tcctcagcgc cttcagtatc
    14641 tgcaggaacg gcgttaattg tcacagtcac tggtttcatt tcataccctc catatgtcaa
    14701 agagttgaat gtgaatgtgt atgtacctgg cttgttggta ctaaatgtac gcgtgatttt
    14761 cccgctcgcg aaatggtcat ttttcgacgg caggaactga taatccgcct ctgtcacccc
    14821 ttccggagcc tcaatgtcga cccacatggt tcctcctacg gggttgacac ccgacatcag
    14881 agtaaccgtc agtgttgcag tagtcttccc gtccgccacc agagaatcta cacgctctgg
    14941 agatgcagcg agcaccgcgt ttttatcatc cgcgacgaat ttagattcgg cggttttcgt
    15001 tgatacctga ccgttgttct gactcacttg cgccgtgagt gtatatgtcg ccgcaacagt
    15061 tgtggtggcg gtgaaaatag cctgcccttg ctcattcgtt gtcgccgtcc cattgggatc
    15121 cagaactaac ttcgccgggc tggcagtcag cgtcacttcg ctatctttca gcaggttgtt
    15181 attggcatca gtcaccgtaa ctttatacgt ctgtttggtt ttgccgtccg ccactgcacg
    15241 gttgccaatc acctcaatac tggagatggt cgccgtagtc ttatcagcca caaacgttac
    15301 agactgcgca ttcgaaggct ggtcatttcc tgctgacgcc gtgatctcat aggttccggc
    15361 agtcacggaa accactttca gttccgcttt accctccttg tcggcgttca ccatgatatt
    15421 accgtctgca agcggtttga cgccccgagg cagattaaag acgaccacag cgccaggaag
    15481 aaggttgccg tattgatcct taaccgttgc cgtcagcgta aaggcatctt tgccgtttgc
    15541 cactttttga gcagcggcgt ctacctccag gctcgcctga ccggtactga aatccgcctt
    15601 gaatttcacc ttgaccgtct tctgagagtt tttcaccaag gcctcgatct caagttcccc
    15661 tgcaaccgtt gacataagct caatgtccac tttacctgcc gcattggttg ttactgtatt
    15721 ccccgtcttg ctagtcacac cctgcggtaa agtcagcgta acttcttgcc cctgcaacgg
    15781 gttaccatag ctgtccacga cggtcagggt tatctggtta gcagactgtc cattagccag
    15841 ttggttatta accttcactg tcatatcacg aatctcagcc ttagatgcgt cacctgcaac
    15901 attcagcacc agtggctgag caacggcatt ttggccgttc actcgcggca tcacagacaa
    15961 ctgacccgcg gcagatccca gcgttaaggt cgacacgtag accccattac ctttctctgt
    16021 ccagtttcct gctgaaggac gctcactccc cgtgctggcg gcaccactaa acactggtgc
    16081 atctggcttc agcccggtga ccggattccc gtacgcatcc ttcacggtga aggtgagttc
    16141 cgtcgtcgtt ttaaccgttg gagttttatt gtccgcaaca agcgtagagt tcgctgatga
    16201 catctctccg gcaataacag tcagctgcgc ggcttcggtg gctgcaggct ggccgttgaa
    16261 gagcggcatg acacgaagct cgcccgtctt tccgcctgta gttaacgtag caacatagga
    16321 accgtcacct ttttcggtcc aactggaaac ggtcgcccct tcagaggcgg tccccgtcaa
    16381 acttgccgac aacgaaagac cactgatagc gttgccatgc gcatctttcg ccaccagcgt
    16441 tacggttgtg ctttcaccgg cttttacgtg atcttctgcg acagagactt tcgactggtt
    16501 tgaagataat gcatcagcca ccacggttac ttttgccgca tttgctgccg cgtcctgccc
    16561 attgagcttc ggcataacgt ctaattcacc cgccgtagtg ccgagagaaa tctgcgcagt
    16621 ccaggtcccg tcgccattat ccgtccagcc tgatgccgta gaaccagcag cagctgcgcc
    16681 cgataatgac ggtgcatccg gattgaggcc agttacaggg ttgtcattag catcttttgc
    16741 cgtccagatt gccgtaactg taccgccaac caccggttta tcaggattca gggtgatgga
    16801 cgaatgtgct gcatcaagcg gcccggcaac aaacttcagc gtttgttgta atgtgcccag
    16861 ctgatactgc tcgctgaagg cacgcacaac aacgtttccg gcacgggtcg aagaaaccgt
    16921 ggcgctgtaa acccctggtt ttatttccga aatggcacca acggttatac cattagtgtc
    16981 ttgcggaaca agtcgcaggc ggctggcttc tcccgtcacc ggattaccct cggagtccac
    17041 cgctgtcagt gtcagcgtgt aggcttgctg accatcagca accacatcac ctgacggctc
    17101 gttagcgctc agggtggagt ttgccacatc catcatcgtg gcccgcagtt ctgcagtgac
    17161 agttttgctc atgtcatcaa cgctaacagt aatcgttgcc tcacctgact gcgttccggt
    17221 agtaaagaca gactgataca cccctgcttc agtttcggtg aactcaccca gtgttggctg
    17281 tgcctgtgat ttagtggcct tcagggtacg agtcacaata tttccagccg gtttgaaggt
    17341 tagttcagtc ttgatctgat ctttcatgcc cgtgactggc tgaccctcgg cgtcgcgcag
    17401 agacagcacc aacggctttt gctcattacc gttagcaagc atttgaatac ggctctgacc
    17461 gtcaagcgtt aacgccgtac gatcggcgct cataccagct ccgctaataa ccacttctgt
    17521 ctgcacacgt ttcgaggcat tgcctttgtt atcgtaggca atcgctgaaa tcgcataata
    17581 attgtctttg cctgcctgat aagccgggag cgtcacttgc cactgattgc cctgccccgt
    17641 aatttttccg cctgcggcca gcaaagacgg cgcttcccat tgcacatttt tcagaccgtg
    17701 agttgctttg ctgaccacaa gccccaggga aaccgtctgg ccgcccttgc cttcaatacg
    17761 ctcaggcaga gcaatacgga tcacttcaga tttgcgatac tcaagaacga tattgttatt
    17821 acgctcaacc aggtcatagc ggctgcctgc cagcattcga cgctcgcgaa tgctgtctgt
    17881 atcgagttgt ttttccagag gttcgccaat ccgataatta acttccaggc caaagcgagt
    17941 gtcattctca ccactcttgc cctgcttatg cccggcactc agggtcagaa gaggcactgg
    18001 cgtgtaattc acttcagcgg taatcgcatg tggatctttc tggcgtttat ctttaccaaa
    18061 cagcccgact tcatcgccat aatactgttc atacatcagg cttgcgccaa gctgcggcca
    18121 ggcaggtaaa tagccctcag cacgaatatc ccagccattc gccgggcgtt cctgataatc
    18181 ctcaacatcc ggcgattttt tccagccaga agcccggata taaccattgg cgctcagttt
    18241 caaataatca cgccagtatt ccgcaccaac accaatgcgg gtatgactac gggataaatc
    18301 atgatcgata aaagtattca cccccgccat ccagtcattt tctgaaaaat gacgccagcc
    18361 aaaaccaata tttgactgag tacgatcgtc ggtacgatgt attgccccct gagtgaacaa
    18421 catatttgtc ggtgtatcat aaatcggata aagcatttcc agcgaagagt ccttcagcga
    18481 gaaattttta tcgacattca gtttgacgcg cgcagtaccg tatttcccga gccactcctg
    18541 aatttcctgg ttagctttag cggtggccat tccggtaata aagttacgtg tcgcatcgct
    18601 atctggctga ctgcttaaaa atgtcccggc attagcggca agcgacgcga catttttctc
    18661 cacgttatta tcagcagtta ccgtagtatt ttccatgctc aaccgtggct gaaccgcatg
    18721 ctgcgcacgt gctgccatta ctggcgtaaa ggtgactgcg agtggaaaaa gaacctgaac
    18781 agagatattt gcccacgcca cgcagcgggc cagaactgaa taacgaaatc gtggctgttt
    18841 attgtctgtt ttataacgtg acattgagat aatttcctga tgaacgaatt aacgaacata
    18901 ggcagacaca aataatggcg tctgcttgtc agaaaaaccg gggatttccc ggtttccttt
    18961 tccacagaaa aggaaatacc tttatgtaag aaagtacacc gccacctaat atatccagtg
    19021 tctgtagcca ctcatcaggt ggttgggcgc gggattatat ttttatttat gggtaaaaat
    19081 ttagcttttt gtgcaagtca ttaaatagat
//
LOCUS       DAZKJR010000100        19043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKJR010000100 DAZKJR010000000
VERSION     DAZKJR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.101"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(105..2771)
                     /gene="cas3"
                     /locus_tag="ACHOWS_003521"
     CDS             complement(105..2771)
                     /gene="cas3"
                     /locus_tag="ACHOWS_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFQ0049045.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNAFCRNEMLSKQKVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSPGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFKEDAPSNINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QLADSVIFALPTQATANAMLSRMEANASRLFTSPNLILAHGNSRFNHLFQSIKSRAFT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPEENN
                     SAYPLINWRGVNGAQRFDLLAHSEQLPPRFSIRPEPIYLADMLPDLRMLERMIVAANA
                     GAQVCLICNLVDVAQLCHQRLKELNNTQVDIDLFHARFTLNDRREKENRVISNFGKNG
                     KRNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEVPL
                     ATVLLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YLQTSSGKQLLDGQVYEDLSHEQQYEALALNRVNVPFTWKRNFSEVVDEDGLLWLEGK
                     QNQDGWIWQGNNIVITYTRDEGMTRVIPANPK"
     gene            complement(3130..3864)
                     /gene="cysH"
                     /locus_tag="ACHOWS_003522"
     CDS             complement(3130..3864)
                     /gene="cysH"
                     /locus_tag="ACHOWS_003522"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFQ0049046.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(3938..5650)
                     /gene="cysI"
                     /locus_tag="ACHOWS_003523"
     CDS             complement(3938..5650)
                     /gene="cysI"
                     /locus_tag="ACHOWS_003523"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFQ0049047.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(5650..7449)
                     /gene="cysJ"
                     /locus_tag="ACHOWS_003524"
     CDS             complement(5650..7449)
                     /gene="cysJ"
                     /locus_tag="ACHOWS_003524"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFQ0049048.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPAN
                     PETSVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            7768..8130
                     /gene="queD"
                     /locus_tag="ACHOWS_003525"
     CDS             7768..8130
                     /gene="queD"
                     /locus_tag="ACHOWS_003525"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFQ0049049.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHYYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            8208..9479
                     /gene="ygcN"
                     /locus_tag="ACHOWS_003526"
     CDS             8208..9479
                     /gene="ygcN"
                     /locus_tag="ACHOWS_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0049050.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGTQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            9470..9730
                     /gene="ygcO"
                     /locus_tag="ACHOWS_003527"
     CDS             9470..9730
                     /gene="ygcO"
                     /locus_tag="ACHOWS_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFQ0049051.1"
                     /translation="MSVTRNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDEKTLQQWRYPPSGFGITYRFG"
     gene            9747..10322
                     /gene="ygcP"
                     /locus_tag="ACHOWS_003528"
     CDS             9747..10322
                     /gene="ygcP"
                     /locus_tag="ACHOWS_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFQ0049052.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(10469..11329)
                     /gene="ygcQ"
                     /locus_tag="ACHOWS_003529"
     CDS             complement(10469..11329)
                     /gene="ygcQ"
                     /locus_tag="ACHOWS_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFQ0049053.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSIRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAEINTMLPSGMLQLNIVPGALPDWLISIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLVEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(11326..12105)
                     /gene="ygcR"
                     /locus_tag="ACHOWS_003530"
     CDS             complement(11326..12105)
                     /gene="ygcR"
                     /locus_tag="ACHOWS_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFQ0049054.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNIGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(12083..13420)
                     /gene="ygcS"
                     /locus_tag="ACHOWS_003531"
     CDS             complement(12083..13420)
                     /gene="ygcS"
                     /locus_tag="ACHOWS_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0049055.1"
                     /translation="MNTSPVRMDDLPLNRFHCRIAALTFGAHLTDGYVLGVIGYAIIQ
                     LTPAMQLTPFMAGMIGGSALLGLFIGSLVLGWISDHIGRQKIFTFSFLLITLASFLQF
                     FATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFSPRRHRGVLLGAFSVVWTVGYVLAS
                     IAGHHFISESPEAWRWLLASAALPALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRY
                     FGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQT
                     IGLEDALTASLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGS
                     SLTLLLFVLFSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPW
                     VLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHSVSV"
     gene            complement(13513..14967)
                     /gene="ygcU"
                     /locus_tag="ACHOWS_003532"
     CDS             complement(13513..14967)
                     /gene="ygcU"
                     /locus_tag="ACHOWS_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFQ0049056.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQVTAQCGVPLEVLENTLREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNHLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(15037..15822)
                     /gene="ygcW"
                     /locus_tag="ACHOWS_003533"
     CDS             complement(15037..15822)
                     /gene="ygcW"
                     /locus_tag="ACHOWS_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0049057.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGKTKEMIEKQGVEMDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            16141..17418
                     /gene="yqcE"
                     /locus_tag="ACHOWS_003534"
     CDS             16141..17418
                     /gene="yqcE"
                     /locus_tag="ACHOWS_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFQ0049058.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            17445..18923
                     /gene="ygcE"
                     /locus_tag="ACHOWS_003535"
     CDS             17445..18923
                     /gene="ygcE"
                     /locus_tag="ACHOWS_003535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFQ0049059.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWTSLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYDHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTILGHITPQAALATHFPAGLSVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMIRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         4615 a         4944 c         4694 g         4790 t
ORIGIN      
        1 accgggaaat taaaagaaga tgtacattgt gcactttccc tacttaagta gggataaacc
       61 gttattggtc ttgttatcgt cattgatacc aatcattccc gaagttattt gggatttgca
      121 gggatgactc tggtcatccc ttcatcccga gtgtaggtaa taacaatatt attaccctgc
      181 cagatccatc catcctgatt ctgtttccct tccagccaaa gtaacccatc ttcatctact
      241 acttcagaaa aattacgttt ccaggtgaag ggtacattga cgcgattaag tgctaacgcc
      301 tcatactgct gttcatgact taggtcctcg tagacctggc catcgagcag ttgtttacct
      361 gaagacgttt gtaaataagg caataatggc aggctcattt ccccatccct cgttaccgca
      421 agaattgttt catcgttatc ctgcaagcta tattcttcag cccactgcag aaccttgcga
      481 gccttgaacc tcttttcaca ctcagcgctt tcaaacttat ccatgccttt gatgacccat
      541 tctggctcat ccatttcagc atcatcgtaa atgctatcca gccattgccg gtaggcatcg
      601 gggaaaaata aggatgctcc attaagttcc tcaatatgtt gctgcgtccg ccacatgact
      661 ctaacgttgc tataaatatg ctcatgtcgt ccgtaacctt cgccatcagg cagcaaaacg
      721 gtggcaagag gaacctcaaa accagcggga cgatatttgc gatgatggcg atgtaaacgg
      781 cccaatcgtt ggaaaagtaa atctgcgggg caatgctgag taattaacca atcaaaatca
      841 acgtcgagtg attgttccac gacctgggtg gcgacaagta tccgtccaac atttcgcttc
      901 ccatttttgc cgaaattgct aataactcga ttctcttttt cacgacgatc gttcagcgta
      961 aagcgcgcat gaaacaaatc tatatctact tgcgtgttat ttagttcctt taaccgttgg
     1021 tggcatagct gtgcaacatc aaccaaattg cagataagac agacttgtgc acccgcattt
     1081 gccgctacga tcattcgctc taacatccta aggtcaggta acatgtcagc taaataaata
     1141 ggttctggcc gaatcgaaaa gcggggcggg agttgttctg aatgagctaa cagatcaaaa
     1201 cgttgtgcac cattcacacc tcgccagtta atgagtggat atgcggagtt attttcctct
     1261 ggatctgtat gcagaccata agtatccaga agtttctgtt tttgtttcat tggtagagtt
     1321 gcggaaagaa gaataacact cccccccaca tcagcctgag ccttgagcac agcctccagc
     1381 aagccgttca tatacgtgtc gtaagcatga acttcatcaa caattaaaac acttcgacca
     1441 attcccaaac cacggataaa gcggtgttta actggcaata ccgatatcaa cacctgatca
     1501 atcgtgcaaa cgccgatttg cccaagaaac actttcttat tgctttgtga caaccactga
     1561 caacactgaa cccatgcttc ttcttgcccc tgctcagtaa atgcgcgtga ttttattgat
     1621 tgaaaaaggt ggttaaaacg tgaattacca tgagcaagaa taagatttgg ggaggtaaat
     1681 aaacggctcg cgttcgcttc cattctgctg agcattgcat ttgcagtagc ttgtgtaggg
     1741 agggcaaaaa taacactatc cgcaagttgt tggtcaataa gtttccaggc ataggccagc
     1801 gcagtttcag ttttacctga acccgtaggt gcctctatta ccgtcagccc gggcgctact
     1861 ggaagagcat caactaacac ttgtaattgt ctgggttgat agccattgtc cagtagagca
     1921 tgaacacctt cataacatcg cttatttgat acaaggccgc tcaactccaa tacccggctc
     1981 gcatcctgct gtcggtcctg gaaatacgtt cttagagcat ttatgttgga aggtgcatcc
     2041 tctttaaaca gaaaggtatt cgttgtagtc caggagccta accagtcagc aagcgagcaa
     2101 aaacctgcta acagtgatga acaatcaggt ggtatatcgt ttatagataa cccggctggc
     2161 gttaaaaata atgcttcgag tacagatatc cactcctcac gagcctgttt atcttgcgca
     2221 gcataagatg ccagagaagc tggcatttcc caacgcgact tatcttgatc ctgggaatgt
     2281 aatataaaac catgatgtcc tgtaacagcc tctacccatg gaaaccagga ctcataagga
     2341 tgaggagcgg catcaaaaaa actgaaaaaa tccccgggag attgctctga aagtgaatcc
     2401 tggttaaacc aatacagacc ggctgcacca tgattaaatt tacggcacat ttgtgttgat
     2461 ggaccattaa gtgatggcgt tgcaggattt aatttcagcc aactttctgc tgatttatat
     2521 tggaatcgta tatcaaactt tccgatatca tgaagggcaa tgaaaaataa cagccaggct
     2581 ttcaccttct gttttgatag catttcattt cggcaaaaag cattttgtag tacgactgat
     2641 tgatcccacc agcaatctgc aacagctgca acatccaggc aatgataaat caacagatga
     2701 atatcatttc ctttcgtcag gctttttgag gatttccccc agtaatgaca tatatattta
     2761 aaaggttcca ttaatagcct ccctgttttt ttagtattat cgatgatatc agtggactat
     2821 ttttcgatgc tgctcataaa tatcgaatta cagtgatata cacatgtctt gcctccctgc
     2881 aaagaagaga ctaacattcg aattgcttag gttcgaacgc tggcctcagg ttgatagaaa
     2941 tatcgcctgg ggcttttgtc catctggaac ctcgcgaatg cttaacgcca gacagcctca
     3001 agcacccaac tccattctat acctgttaat tctccctgcg atgttcacct ttaaatgccg
     3061 gaatgctgct cgcaaaataa aaacgccccc atcatttctg acagaggcgt ttaatttatc
     3121 cggcaatatt tacccttcgt gtaacccaca ttcccttttt aagccaaaga aacgcgtttc
     3181 ttcttccgcc atgccgggtt cccatttacg ggttgtatgg gtatcaccga ccgataaata
     3241 tccttcgtcc cataatgggt gatatttcag gccatgtttt tgcaggtact gataaatagt
     3301 tcggttatcc cagtcgataa tcggcagcac tttaaatacg ccacgctgaa ttgccagcac
     3361 cggtaaattg gcgcggctgc cggattgttc acggcgcagg ccagcaaacc aggtttgcgc
     3421 attcagttct ttcagcgccc ggttcatcgg ttcgactttg ttgatctcgt tgtacttctc
     3481 aatcccctca acaccctgct cccacagttt accgtagcgc gcttcctgcc aggcggcgct
     3541 ttcggtagcg cggtacactt tcaggttgag cttgagtttg tccgttaact cgtcaataaa
     3601 gcggtaggtt tctgggaaca gatagccggt atcggtgagg atcaccggaa tatccgggcg
     3661 aatctgattc accagatgca ggctcaccgc cgcctgaatg ccaaagctgg aagaaagtac
     3721 atattcaccg ggcagattat ccagcgccca ggcaacgcgg ccttcagcgt ccagtttttc
     3781 cagttcggcg ttagtttccg ccagcgccag aatgcgatct accttcggca gttcgttcag
     3841 ggcgtttaga tcgagtttgg acatagattc ctcactgttt gccttgcctg atgcgacgct
     3901 cacgcgtctt atcaggccta caagaccggg ctaatggtta atcccacaaa tcgcgcgccg
     3961 gatcgagcac cgggcgaatg atgcccgcac gcaccgtaaa gtcgccgaag ccttcacccg
     4021 cttcgcgctc tttcgcccag cgccctatca gttcatcaag cgacgccagg atttccggct
     4081 cggtgatgtt ttctttatac atccgtggga tacgtgtccc aatgcggttg ccgccaagat
     4141 gcaagttgta gcgacctggc gctttaccca ccaggcccac ttccgccagc atcgcgcgac
     4201 cacaaccgtt cgggcagcct gttacacgca tcacgatatg ctcatcgctg acaccatgtt
     4261 tcgccattaa attatcgatg ttgtcgataa aagacggcag gaaacgctcg gcttcggcca
     4321 tcgccagcgg gcaagtcggg aatgacacgc aggccattga gttttcacgc tgcggcgtga
     4381 cggcattcat taacccgctc tctttggcga tcttctcgat cttcgctttc tcgctttccg
     4441 gcacaccggc gatgatcaga ttctggttcg ccgtaatgcg gaaatcgcct ttgtggatct
     4501 tcgcgatctc cagcaggccg gttttcagcg gacgcccagg ataatcaagg atgcgaccat
     4561 tttcgataaa cagcgtcaga tgccagttat catcaatgcc cttaacccag ccaatacgat
     4621 cgccgcgtcc ggtaaactca tacggacgga tcggttcaaa tttgatcccc gcgcgacgct
     4681 ccacttccgc tttaaacgtc tcaaccccca cgcgctccag cgtgtatttg gttttggcat
     4741 ttttacgatc ggttcggtta ccccagtcac gctgagttgt cacgacggct tccgccactg
     4801 ccaacgtatg ctccagcggc agatagccaa actcactcgc ggtgcgggcg taggttttct
     4861 tgttgccgtg ttcgatggaa agcccaccgc ccaccaacag gttaaagccc accagcttgc
     4921 cgttttcggc gattgccacg aagttcatgt cgttggcgtg cagatcgata tcgttctgcg
     4981 gcgggatcac taccgtggtt ttgaacttac gcggcaggta ggtctgacca aggatcggct
     5041 cttcatcagt agtggcgact ttttcctgat cgagccagat ctccgcatac gcgcgggtac
     5101 gaggcagcag atgttcagag attttcttcg cccattcata tgcttccgcg tgcagttgcg
     5161 actcgtaagg gttcgaggtg cagagtacgt tacggttcat gtcgttagca gtcgccagcg
     5221 catcaaggcc gaccgagtgc agcatctggt gcaccggttt gacgttcttt ttcagaatgc
     5281 cgtggaactg aaacgtctgg cggttggtca ggcgaatgct gccatagatg gtgttttcac
     5341 cggcaaattt gtctatcgcc tgccactgtt tggtggtgat cacgccaccc ggcagacggc
     5401 agcgcaacag catcgcgtgg cgcggctcca gcttctgttc agcacgttcg gcgcggatgt
     5461 cgcggtcatc ctgctgatac atgccgtgga agcggatcag caggaagttg tcgcccttaa
     5521 agccgccggt cagaccgtcg tttaaatctt ccgcaatggt gccgcgcagg tagttgcttt
     5581 caagcttcat gcgctcggcg tctgtcagtt ttccttcgac cactaaaggc cctggatgtt
     5641 tttcgctcat tagtagacat ctcgctgata acggcgctct acgcgcagct cacttaaaaa
     5701 ttcatccgcc gcttcggtgt ccatgccacc aaattcggca atcacttcca gaagtgcctg
     5761 ctcaacgtct ttcgccatgc gattagcgtc gccgcagaca taaatgtggg caccgtcatt
     5821 gatccagcgc cacagctccg cgccctgttc gcgcagtttg tcttgtacgt aaattttttc
     5881 tttttgatcg cgcgaccagg caagatcgat acgcgtcagc acgccctctt tgacgtagcg
     5941 ctgccattcc acctggtaga ggaaatcttc cgtaaagtgc gggttgccaa agaacagcca
     6001 gtttttcccc ggcgcctcgt cggcggcgcg ttgctgcata aaggcgcgga acggcgcgat
     6061 gccggtgcct gggccaatca taatcaccga cgtttccgga ttggctggca ggcggaagtt
     6121 gtcgttatgt tcgataaaga cgcggacttc accatcttct tccacacgat ctgcgaggaa
     6181 gctggaggca ccaccggcac gggcgcggcc ttccacgtcg taacgcacca caccaacggt
     6241 aacgtgcact tcgttctcga cttccgcctg cgaggaggcg atggaataca ggcgcggggt
     6301 cagcgggcgc agcaggttaa tcagcgcttc ggcatccagt tgcgccggag agaaacgcac
     6361 catgtcaaca atcagcgtgg tcgcggcgta atgctgtaac ttcgctttat cgcccaccag
     6421 cggcagcagc gtttcgctgc gggtaagcgt ggcgtagttc tcaacaatat tcgcggtgtt
     6481 gacggtcagt tcaaagtgcc actgtagcgc ttcgctcagg gtcagcgttt tgccgtcaac
     6541 ggtgacaggt tcatcgcctt tcagccacag cagttcgaca agttctttca ccagtgccgg
     6601 atcgttctga taccagacgc ccagcgcgtc acccggctgg taacgcaggc cagagtcacc
     6661 taagtcaatt tcgatatggc gaacgtcttt ttcagagtta cgcccggtaa ttttctggtt
     6721 aacagagagg ctcgccacca gcggcgcgtc tttgctgtac gggctggtgt ggatttcatt
     6781 taccgcgcca gtagcgacgg attgcgaagg tgccgcgaca ggcgcacgcg atttaagcgc
     6841 atcaaccacg cgggcgcgcc actcgatggc agcagcctgg tattcaacgt cggcatcgac
     6901 acggtcgagc aggcgttcac cgcccagttc cgccagctta ctgtcaaaat ctttcccgga
     6961 ctggcagaaa aattcataag aactatcgcc gaggctaaac acggcaaaag cggtattttc
     7021 cagcttcggc gcttttttgg agaacaggaa cttatgcagc gcgacggctt cttccggcgg
     7081 ctccccttcc ccttgcgttg acgtcactac gatgagcagt ttttcgctgg cgatttgttt
     7141 gaatttatag tcgcccgcgt tcaccagctt aacgttcagt tttgctgcta ataaatcatc
     7201 gcgtaatgct tcagcaaccc ggcgcgcatt gccggtttgc gaggcggaga taatagttat
     7261 acccggcatt tctgcggctg gcgctggcgt cgctgcaagc gcagcaggct gctgattgag
     7321 tacgccccag aaatagccag aaacccaggc aagctgggta ggagttaaat cggtcgtggc
     7381 cgcctgaagg cgtgccagtt gctccgggtt caacggaagc aacgcggaag gtgggacctg
     7441 tgtcgtcatg cgtcgttatg ttccagtaag caaagctgtt tctgcgccct gtcagcgccc
     7501 ataaaacaga agaggaggta aggttaacgg ggtgaacggt gtggattaaa gacgggatag
     7561 cgataactaa taaccaaatc gactaacctg ttttagcaat agtctttaac aacaaaacag
     7621 attaaccaac ccaatgaaaa acaaatgaat ttagccaatc attaagataa atcagcgatt
     7681 ttgcgcaaca aagtcgtttt agataatgcg aaaaaacagc ctttccggta ctctacggcg
     7741 gttttatcgt cctgtagaga aattatgatg tccaccacgt tatttaaaga tttcaccttc
     7801 gaagccgctc accgcttacc acacgtcccg gaagggcata aatgtggtcg cctgcacggg
     7861 cattccttta tggtgcgact ggaaattacc ggggaagtcg atccgcatac gggctggatt
     7921 atcgatttcg ctgaactgaa agcggcgttt aagccaacct acgagcgcct cgatcactat
     7981 tatctcaatg atattccagg tctggaaaac ccgaccagcg aggttttagc aaaatggatt
     8041 tgggatcaag ttaaacccgt tgtgccgctg ttaagtgcgg tgatggtaaa agaaacctgc
     8101 accgcaggtt gcatctatcg cggcgaatga taagagtgtg tcggcggtca atttccctta
     8161 agtaacgcta tgttagggtg ttgtgttctg gatatctggg gcatgacatg gaagacgact
     8221 gcgacattat tattattggt gccggtattg caggcaccgc ttgcgcgtta cgctgcgcgc
     8281 gagcgggttt atccgttttg ttactggaac gcgctgaaat ccccggcagc aaaaatcttt
     8341 ccggcgggcg gttatatacc catgcactcg cggaactcct ccctcagttt cacctgaccg
     8401 cgcctcttga acgacgcatc actcacgaaa gcctttccct gttaacgccg gatggcgcaa
     8461 cgacgttttc cagcttacag cccggcggtg aatcctggag tgtattacgt gcacggttcg
     8521 atccgtggct ggttgccgaa gccgaaaaag aaggtgtcga atgcatcccc ggtgcgacgg
     8581 tggatgcgct gtatgaagaa aacggcaggg tgtgtggtgt catttgtggt gacgatattc
     8641 tccgcgcccg ttatgtggtg ctggcagaag gtgccaacag cgtcctggct gaacgtcacg
     8701 ggttagtgac tcgtcctgct ggcgaagcga tggcgttggg gatcaaagaa gtgctgtcgc
     8761 tggaaacatc cgctattgaa gaacgttttc atctggagaa taacgaaggc gcagcgttgc
     8821 tgttcagcgg cgggatctgt gatgacttac ccggcggcgc atttctttat actaatcaac
     8881 aaacgctctc gttagggatt gtttgcccgc tctcttccct tacgcaaagt cgtgttccgg
     8941 caagcgagct gctggctcgc tttaaaacgc atccggcagt gcgcccgctt atcaaaaaca
     9001 cggaatcact ggagtatggt gcgcatctgg tgccagaagg tggcttgcac agtatgccgg
     9061 tgcaatacgc cggtaacggc tggctgctgc tgggcgatgc gttgcgcagt tgcgtcaata
     9121 ccggaatttc cgtgcgcggc atggatatgg cgctgactgg cacgcaggcg gcggcacaaa
     9181 cgctgataag cgcctgccag caccgcgagc cgcaaaatct gtttgcgctt tatcatcaca
     9241 acgtagagcg cagcctgcta tgggatgttc tacaacgtta tcagcatgtt ccggcgcttt
     9301 tgcaacgccc tggatggtac cgtgcgtggc ctgcgttaat gcaggatatt tcccgcgatt
     9361 tatgggatca gggtgataaa cctgttccac cgctgcgcca gttattctgg cgtcatttac
     9421 gtcgtcacgg cctgtggcat ctggcgggcg atgttatcag gagtctgcga tgtctgtaac
     9481 ccgtaatctc tggcgcgttg ccgatgcgcc gcacattgta ccggctgact ccgttgagcg
     9541 tcagacggta caacggttga ttaacgcttg tccggctggt cttttttcgc tcacaccgga
     9601 aggtgactta cgcgttgatt atcgcggctg cctggagtgt ggcacctgcc gtttgctgtg
     9661 cgatgaaaaa acactacaac agtggcgcta tccgccttcc ggattcggca tcacctaccg
     9721 ttttggataa ataataagga taatttatgc ccctcttaca cctgctccgc cagaatccgg
     9781 tgattgctgc cgttaaagat aatgccagcc tgcaactggc aatcgactct gaatgccagt
     9841 ttatttccgt gttgtacggc aatatctgca ccattagtca aatcgttaaa aagattaaaa
     9901 atgccggaaa atatgctttt attcatgttg atttgctgga aggcgcgtca aataaagaag
     9961 tggtgattca gtttttgaaa ctggtgactg aggcggacgg cattatcagt accaaagcct
    10021 caatgttgaa agcggcaaga gcggaaggtt ttttctgtat tcatcgcctg tttattgttg
    10081 attcgatttc gtttcacaac attgataagc aagttgcgca atcgaatccg gattgtattg
    10141 agatcctgcc aggctgtatg cctaaagtgc tgggctgggt gacggagaaa atccgccaac
    10201 cgctgattgc cggcgggctg gtgtgcgatg aagaagatgc gcgtaatgcg attaacgcgg
    10261 gtgttgtggc gctttccacc acgaataccg gggtctggac gttagcgaaa aaattgcttt
    10321 gacgggatac tcgtaacgag ttgaatttgt gtgatttgta ggctgcacgc cacatccgac
    10381 attcagcgcc tgatacgacg cttgacgcgt cttatcaggc ttacaagtcc ccagcccaga
    10441 accgtaggca ggatcatccg gcagcacgtt attggcaatc tgcgtgaatg gttgtcacca
    10501 gtgcctccag caccgctttc cagtcatcaa ccacgccaac atctgcctgt gaaaataccg
    10561 ccgcgctggc gtcatgatta atcgccacca caaacttgct attgcgtacg cccgccatta
    10621 acgctgctgc gccggaagcg ccaacaacaa tgcacacctc aggcgccagc agatgtccgg
    10681 aaataccaat cactttttca gcatcaacgc caccgttcat cactcgcgcc cgactgtagc
    10741 ccacttcggc tcccagcttt tctaccagca tagcgatttc ctggttatct gcctctccgc
    10801 cctgccccac caccagcacc cgtctggctt cagctaatgg atcgcgggta acatttttca
    10861 ggttttctat gctaatcagc cagtcgggaa gcgcaccggg gacaatgttc agttgcagca
    10921 taccagaggg caacatggtg tttatctctg ctcccgcctg acgcgccagg gaaagacata
    10981 gcgggcgttt ttctgtttgc agcgtcgccg ttagcgcatt tccccagtgg gatttgcgca
    11041 cgcttacggt tggaatatct aacgaagtca cctggcagat gcttgcacca tgtaaacgcc
    11101 aggccagccg gatcgacagt tcatcgccaa acgcgcccgg aggaaaaagg acaacctccg
    11161 caggcgttcg ttgccactgt tccaccagcg catccagcac ctgttccgca accatcggct
    11221 gtggttctat ttgccaatgc gttagtgtgc tgccgctgaa atcctgcgcc gccagccagc
    11281 ccgccattgc ggcatgttcc tgatttattg tcacaattgc gatattcatg gctgcatcct
    11341 ttggcgtaaa tagtcctgcc atagcttttg cgctttttct gctaccgttt gcccatcaat
    11401 cagcgccgct ctgcgtcgtt gctctggcct tgccagttgc aggcactgta tcgctggcgc
    11461 ttccgcggca acagtttcgc gggtaatctc cgctttcccc gctgccattc gctggcgcat
    11521 tcccggtact ggcaaggcca cttcaccaca ctgacgcacg gcaatcaccg caggcaagcg
    11581 aacccggcaa cagcgcaacc catgttcagt acgctgttcg agggtgataa acggcgcgtc
    11641 gagagtaaaa cgctccacct gggtgaaaca gggccagccc agcatttccg ccagcaaaaa
    11701 tggcgtttgt ccattctgcc cttcgctgct ttggcagccg gtaatgatca gatccagcgg
    11761 attctgacgc tgccattcgg caatatggcg agcgacaaat tccggcgcaa agcgcagatc
    11821 tgccgccgtc tccagcaata cagcgtcctc aaacccaaga gccatgagat agcgcagcca
    11881 gtgcaacgcc cgttcatccc ccatgctcaa cgcggttaaa gacatcggcg taccgttttt
    11941 tctctgcgcc aacagcagcg cggcggcagc ctgttcatcg gcaccgagta aacttcgcag
    12001 aagcgaaaca tccggtccga tattaccctg agccgccgcc tgccactctt tttccgccag
    12061 cattccggca tccggttcgg ctttaaacgc taacagaatg ttcattcgca cctcctacat
    12121 ttcccgccgc caccagcggg agtgctttgg tttctggtgc ccatagccag gtcacaacaa
    12181 aaccaaccaa caacactgcc gccaggagca ataaggtggc ttgcattccc cactgcgcca
    12241 gcacccacgg cagcaggcca gtacttaccg ccgcccccaa ccgactcatg gcggtggcaa
    12301 aaccgacgcc cagcgagcga atgtcagtag gaaaactttc cgcaggcaaa atgcccacca
    12361 gattactgac tgccgaaatg gtggtgctga agagaacaaa aagcagcagc gttaatgaac
    12421 tgccggaagg caaacaggcc atcacgacca gcgttgccgc cagcagcaaa aaacttccca
    12481 gtaaaaatct gcgatgcgct aacaagtggg tcaacactaa ccctaataac gcgccaacaa
    12541 ttaacaacgc attaagcatc aggctggcag tcagcgcatc ttccagacca atagtttggg
    12601 cgatggtcgg cagccaggta taaatcacaa accatgggat tacgaggcag acaaagaaga
    12661 cgctgttaaa cgctgtgcgc cgccagtagc gcgaagagaa caaggttttg atgtgtttat
    12721 gggtcgccgt tgccacttca tcgcccagta aaacatgggg accaaaatag cgatgcacga
    12781 tagcgtgagc ttctgcaaaa cgcccctggc gcagtagcca gcgtggcgat tctggcgttc
    12841 cccagcgtaa taacgtaatc aacaatgcgg gcagagctgc cgatgccagt agccagcgcc
    12901 aggcctccgg gctttcgcta ataaagtgat gtccggcaat acttgccagc acatagccta
    12961 cggtccacac cacgctgaat gcgcccagca aaacaccgcg atggcggcgc ggagaaaatt
    13021 cagccagcaa agtgtgaccg actgagtaat cgcctcccag accaatgccg atcaaaatgc
    13081 gcagtccaat aagatgctct ggcgtggtgg caaaaaattg taagaacgaa gcgagcgtaa
    13141 tcagcaaaaa gctgaaggtg aagatttttt gccgaccaat atggtcggag atccacccca
    13201 aaaccaggct accgataaac aggccaagta gcgccgagcc gccgatcatt cccgccataa
    13261 acggcgtcag ttgcatggcg ggtgtaagct gaataatggc gtaaccaatg acgccgagga
    13321 cataaccgtc ggtcaggtgt gcgccgaaag tgagcgcagc aatgcggcag tgaaaacggt
    13381 taagtggtaa atcatccatt cgcaccggtg aagtgttcat ctttacccct tcattgtccg
    13441 gtaataagaa cggcttctcg cctgagaagc cgaaaaggca acgcccgtca tcgcggaacg
    13501 ctgcctgaca cattattttt caatcggata gatagtaccc gtattcataa tgccgttagg
    13561 atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt ccagtttgct
    13621 ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc atcgaaccac cgaggcgaat
    13681 ggtttcttcg cagatgattt tgttgagcgg attgtggtac ttgtcgattt cctcttccgg
    13741 cttacagtca acgacgttgt agtcgtagac gaagtacatg ttggtgccgt tctgatagct
    13801 atgagaggag tgaccgccca gcatggtgat gtcgtcggca tgcgggaact cggtacgaat
    13861 acggttaata acgctttcgt agatttcgtg gatgcagctc cagcagccgg acacttcggt
    13921 ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcagcggcca ctttatccgg
    13981 cccccagttc aggtgattaa accaggtttc gatcagcttg ctgtccactc gctggcattg
    14041 cgggtaacgt gccacgattt cagcaatccc ttcgcctgtc gccttcgcaa tgcgagggtt
    14101 accttcagcc ataaagatca gcacacattt accatcggca aaatgggtga agtgttgggt
    14161 gccatcttca gcgtcataca aacgagcgat cgacggacga tacccttcca ccatgatttc
    14221 acgcaggatg ttgaagccgg ttttcatgtc ttccagcaca tagccataga agagattgtt
    14281 ttccggcgta aatttgaaga ttttcactgt gacttcagtg atatagcaca atgcaccttc
    14341 gttgccgatg ataatgtgac gaatgtccgg gcctgccgcg cggcgtggta cgtttttaat
    14401 acgtgtgacg gtgccatcag ccaatactgc ttccagacca acgaccatat cttcgattgc
    14461 gccgtagagt gtggagaact gcccgatact gcgggttgct accaggccgc ccatctgcgc
    14521 cagcggtttt gattgcggtg aatgccccgt ggtgtaacct ttttcacgca acgtgttttc
    14581 cagcacctcc agcggaacac cacattgcgc cgtgacctgc atattctcaa tatcaatatt
    14641 aatgatttga ttcatggcag agccgtcgag caccaccgag ttttccacaa cggtttccag
    14701 tccaccctcg gtagcggaag caccggtacg cggcacaccg ttaattttgt gtgcattcat
    14761 aaaattcagt acacgagaga cttgttctgt ggaaccgagt tttacgaccg ctgccggaat
    14821 cggcaaagta taaatgccat gaatatccgg aaatttgcga aaacggtcaa tactgttttt
    14881 ctttaatact gtttcatcgg taattacgcg atctgcacca acaatttcct ttagctggtc
    14941 gacaatcgct gcgcgagata aagacatagt aattccttcc tgataataaa aatggaataa
    15001 tcattaataa gagaaaacag aaaccaccgt tatggattag cgcactaaat aaccgccatc
    15061 gaccactaat aaatgcccgt tgacataatt cgatgcctga cttgcgagaa acacggctgc
    15121 gcccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa gaacgcgctg
    15181 attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat agccaggggc
    15241 aataccattt acctgaatat tatattgacc tagttcatca caataagctt tggtgaaccc
    15301 ggcaagagca tgtttagtgg cagaatatgc aggtgaccat tgtccaccta agtaagagaa
    15361 caatgaacag atattaataa ttttaccgct tttctgcggg atcataattt ttgccgcttc
    15421 atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcgggtccc agtcggcacg
    15481 accgaaatcc agcaccttat tcagcttaca aataccggca ttgttaacca gaatatcaac
    15541 tgttccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt ctgcggtgat
    15601 atccacctgc atgaagtcca tctcaacacc ctgtttttca atcatttcct ttgttttgcc
    15661 gttatcttta acgaagcttg gaataaagat atttgcacca gctttggcca gcgccatggc
    15721 aaatgcctgg cctaaaccgc tattcccgcc ggtaacaatt gcggttttac ctttcaggga
    15781 gaaaaaatcc attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg
    15841 taaggcaaaa aaaaagaaag gtaacttccc ccgcgagagg gaagttacct ttctcattat
    15901 ctctggtaac tggagataat ttttatcata agggcataat cgctaatgtg atcgtaatca
    15961 cagtgttata ttcgaattgt caaataattt taaaaagcag aagcgtgatt actctcacac
    16021 aacaccactg ctttccatgt tatttcttat tgcaattact agagacctga gaaaagtaat
    16081 ccacctgaaa gggcggctta cttttctctt ttttttgtct attacaaatg ggaatacgat
    16141 atgcaacaca actcatatcg ccgttggata accctcgcga taattagttt tagcggcggc
    16201 gttagtttcg atctggctta tttacgttat atttatcaaa ttcccatggc gaaatttatg
    16261 ggattcagca ataccgagat aggtttaata atgagtacct ttggtattgc ggctattatt
    16321 ctttatgccc ccagcggcgt tattgccgat aaattttcac accgcaaaat gattacttcc
    16381 gcgatgatca ttaccggatt actgggtctg ttaatggcaa catatccacc gctgtgggta
    16441 atgctctgta ttcaggtcgc ctttgcgata acgacgattt taatgctgtg gtcggtgtcg
    16501 attaaagccg cctcattgct gggcgatcat agtgagcaag ggaaaattat gggctggatg
    16561 gaagggctgc gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct
    16621 cgctttgcgc cggatgacag cgccagcctg aaaacagtca ttattatcta cagtgtggtt
    16681 tacatcttgt tggggattct gtgctggttt tttgttagcg ataacaacaa cctgcgcagt
    16741 accaataacg aagaaaaaca gtcattccag cttagcgata tcctggccgt tttgcgtatc
    16801 agtaccacct ggtattgcag catggtgatt tttggtgtct tcaccatcta cgccattctg
    16861 agttactcca ccaactatct gaccgaaatg tacggcatgt cgctggtggc ggcgagctac
    16921 atggggattg tgatcaacaa aatcttccgc gcgctgtgcg gcccacttgg cggcattatc
    16981 accacctaca gtaaagtgaa atcccctacc cgcgtgatcc aaatcctttc cgtactcggc
    17041 ctgctggcgt taactgccct gctcgtcacg aactctaacc cgcaatcggt cgcgatgggg
    17101 attggcctga ttttactgct gggattcacc tgttacgcct cacgcgggct gtactgggcc
    17161 tgcccaggcg aagcgagaac accgtcttac attatgggca ccacggtagg tatttgttcg
    17221 gtgattggat tcctgccgga tgtcttcgtt tacccaatta tcggccactg gcaagacacc
    17281 ctgcccgcag cagaagccta ccgcaatatg tggctgatgg gcatggcggc gcttggcatg
    17341 gtgattgtct ttaccttttt gctgttccaa aaaattcgta ctgctgatag cgcccccgca
    17401 atggctagca gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg aaaaaataca
    17461 tcatagggat tgatggcgga agtcagagca caaaagtggt gatgtacgat ctggaaggta
    17521 atgtggtttg cgaaggtaaa ggcttattgc agccgatgca tacgccagat gccgatactg
    17581 ccgaacatcc tgacgacgat ttatggacat cgttatgctt tgccggtcac gatttgatga
    17641 gccagtttgc cggaaataaa gaagatattg tcggtattgg tctgggctcc atccgttgct
    17701 gtcgtgcgtt attgaaagcc gatggcacgc ccgctgcgcc attgattagc tggcaggatg
    17761 cacgtgtaac acgtccctac gatcatacca atccagatgt ggcctatgtc acctcttttt
    17821 cgggttatct gacgcatcgc ttaaccggcg agtttaaaga taatatcgcc aactattttg
    17881 gtcagtggcc ggtggattat aagagctgga catggagtga agatgctgcg gtaatggata
    17941 agtttaatat cccccgtcat atgctgtttg atgtgcaaat gcctggcacc attcttgggc
    18001 atatcacacc acaagctgcg ctggcgacac atttcccggc tgggctgtcg gttgtttgta
    18061 ccaccagtga taaaccggta gaagctctgg gagccgggtt actggatgat gaaactgcgg
    18121 taatttctct tggcacttac atcgcattga tgatgaacgg caaagccttg ccgaaagatc
    18181 cggtggcgta ctggccgatt atgtcttcta ttccgcaaac attgctgtat gaaggttacg
    18241 gtattcgcaa aggcatgtgg acggtgagct ggctgcgcga catgttaggc gagtcgttaa
    18301 ttcaggatgc cagggcgcag gatctttcac cggaagattt actcaacaag aaagcctcta
    18361 gtgtgccacc tggctgtaat gggctgatga cggtgctgga ctggctgacc aatccgtggg
    18421 aaccgtacaa acgcgggatt atgatcggct ttgattccag tatggattac gcatggatat
    18481 atcgttcgat actggaaagc gtggcgctga cgctgaagaa caattacgac aatatgtgta
    18541 atgaaatgaa tcactttgcg aagcatgtaa tcattactgg cggcggttcg aacagcgatc
    18601 tgtttatgca gatttttgcc gacgtgttca accttccggc acgacgtaac gccattaacg
    18661 gttgtgcaag tctgggggca gcgattaata ccgcggtagg tctggggcta tacccggatt
    18721 acgcaacggc tgtcgacaaa atgattcgcg tgaaagatat ctttataccg attgagagca
    18781 atgccaaacg ctacgacgcg atgaataaag gcattttcaa agacctaacc aaacatactg
    18841 atgtgatcct gaaaaaatcg tatgaagtga tgcatgggga attggggaat gcggattcga
    18901 tccagagctg gtcgaatgcg taagtgagga aggccgggcg ggaaactgcc cggcctgaac
    18961 atacctgaat ggttatcccc gctgacgcgg ggaacataag ttcgacaact taaaaaatga
    19021 taacaagtcc ggtttatccc cgc
//
LOCUS       DAZKJR010000101        18634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKJR010000101 DAZKJR010000000
VERSION     DAZKJR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.102"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..843
                     /gene="yieP"
                     /locus_tag="ACHOWS_003536"
     CDS             151..843
                     /gene="yieP"
                     /locus_tag="ACHOWS_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0049060.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAMVGTAEQKAHLNTLMTEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            866..2293
                     /gene="mdtD"
                     /locus_tag="ACHOWS_003537"
     CDS             866..2293
                     /gene="mdtD"
                     /locus_tag="ACHOWS_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFQ0049061.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2259..3251)
                     /gene="rbsR"
                     /locus_tag="ACHOWS_003538"
     CDS             complement(2259..3251)
                     /gene="rbsR"
                     /locus_tag="ACHOWS_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFQ0049062.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLLHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3255..4184)
                     /gene="rbsK"
                     /locus_tag="ACHOWS_003539"
     CDS             complement(3255..4184)
                     /gene="rbsK"
                     /locus_tag="ACHOWS_003539"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0049063.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4310..5200)
                     /gene="rbsB"
                     /locus_tag="ACHOWS_003540"
     CDS             complement(4310..5200)
                     /gene="rbsB"
                     /locus_tag="ACHOWS_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFQ0049064.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5225..6190)
                     /gene="rbsC"
                     /locus_tag="ACHOWS_003541"
     CDS             complement(5225..6190)
                     /gene="rbsC"
                     /locus_tag="ACHOWS_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFQ0049065.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6195..7700)
                     /gene="rbsA"
                     /locus_tag="ACHOWS_003542"
     CDS             complement(6195..7700)
                     /gene="rbsA"
                     /locus_tag="ACHOWS_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFQ0049066.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7708..8127)
                     /gene="rbsD"
                     /locus_tag="ACHOWS_003543"
     CDS             complement(7708..8127)
                     /gene="rbsD"
                     /locus_tag="ACHOWS_003543"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0049067.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPHLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8294..10162)
                     /gene="kup"
                     /locus_tag="ACHOWS_003544"
     CDS             complement(8294..10162)
                     /gene="kup"
                     /locus_tag="ACHOWS_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFQ0049068.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10385..11881
                     /gene="ravA"
                     /locus_tag="ACHOWS_003545"
     CDS             10385..11881
                     /gene="ravA"
                     /locus_tag="ACHOWS_003545"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFQ0049069.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11875..13326
                     /gene="viaA"
                     /locus_tag="ACHOWS_003546"
     CDS             11875..13326
                     /gene="viaA"
                     /locus_tag="ACHOWS_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFQ0049070.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCMALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13331..14323)
                     /gene="asnA"
                     /locus_tag="ACHOWS_003547"
     CDS             complement(13331..14323)
                     /gene="asnA"
                     /locus_tag="ACHOWS_003547"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFQ0049071.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14475..14933
                     /gene="asnC"
                     /locus_tag="ACHOWS_003548"
     CDS             14475..14933
                     /gene="asnC"
                     /locus_tag="ACHOWS_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFQ0049072.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15023..15466
                     /gene="mioC"
                     /locus_tag="ACHOWS_003549"
     CDS             15023..15466
                     /gene="mioC"
                     /locus_tag="ACHOWS_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFQ0049073.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15845..17734
                     /gene="mnmG"
                     /locus_tag="ACHOWS_003550"
     CDS             15845..17734
                     /gene="mnmG"
                     /locus_tag="ACHOWS_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0049074.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17798..18421
                     /gene="rsmG"
                     /locus_tag="ACHOWS_003551"
     CDS             17798..18421
                     /gene="rsmG"
                     /locus_tag="ACHOWS_003551"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0049075.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDGERHLVVIKANKI"
BASE COUNT         4681 a         4847 c         4607 g         4499 t
ORIGIN      
        1 ggaaatttaa aataattttc tgaccgcgca ttttttcagc aaaagccccg ttaatgtgcc
       61 ataaaacaag caatttgccg tttttgcaac cgtaatcaca cttcctggtg gcatactaag
      121 gaggaataaa aagaaaaagg aagattagta atgcctttaa gcgcacaaca gttagctgca
      181 cagaaaaacc tttcgtatgt tctggctgag aagctggcgc aacgcatctt aaaaggtgaa
      241 tatgaacccg gcaccatttt gcctggtgag attgagctgg gcgagcaatt tggagtgagt
      301 cgtacagcgg tacgcgaagc ggtcaaaacg ttaacggcaa aagggatggt tttaccgcga
      361 ccgcgaattg gtacccgggt catgccacaa tcaaactgga attttcttga tcaggaattg
      421 cttacctggt ggatgacaga agagaacttt catcaggtca tcgatcactt tctggttatg
      481 cgtatctgtc tggagccgca agcttgccta ctggcagcaa tggttggcac cgcggaacag
      541 aaggcgcatc tcaatacgtt aatgaccgaa atggtggcat taaaagagaa ctttcgccgt
      601 gaacgctgga ttgaggtcga tatggcctgg catgagcata tctatgaaat gagcgccaat
      661 ccgtttttga cctcattcgc ctcgctcttc cattcggttt atcacactta cttcacgtca
      721 attaccagcg acacagtgat aaagctggat ctgcaccagg cgattgttga cgcgattatc
      781 caaagcgatg gcgacgcggc atttaaagct tgtcaggcgc tgctacgctc acctgataag
      841 tgacaccccg ataacaggac attgaatgag cgataaaaag aagcgcagta tggcgggttt
      901 gccgtggatc gcggcgatgg ccttctttat gcaggcactt gatgccacta ttctgaatac
      961 cgccttaccc gcaatcgctc atagccttaa tcgttctcct ctcgcgatgc aatcagccat
     1021 catcagttat acgctgacgg tggcgatgct tattccggta agcggatggc tagccgatcg
     1081 cttcggtacg cgtcgcatat ttactcttgc cgtgagtctg ttcacgttgg gttctctggc
     1141 ctgcgcactt tctaactcgc taccacagct ggttgtcttc cgggttattc aggggatagg
     1201 cggcgcaatg atgatgcctg ttgctcggct ggccttactg cgcgcttatc ctcgtaatga
     1261 gcttcttcct gtattgaatt ttgtcgccat gccgggtctg gtggggccaa ttttaggccc
     1321 tgttcttggc ggcgtgctgg tcacctgggc aacctggcac tggatatttt taatcaatat
     1381 ccccataggt attgcgggcc ttctttacgc gcgcaaacat atgcccaatt tcaccaccgc
     1441 acgacgcaga ttcgatatca ctggcttttt gctgtttggc cttagccttg ttctcttctc
     1501 aagcggaata gagctattcg gggaaaagat tgtcgccagt tggattgcct tgacggtaat
     1561 tgtcaccagc atcgggttac tgcttctcta tattctccat gcacgacgca cgccaaaccc
     1621 attaatttca ttagatttat ttaaaacccg cactttctcg atcggtatcg taggcaatat
     1681 tgcaacccgt ctggggaccg gttgtgtacc gttccttatg ccattgatgt tacaggtagg
     1741 atttggttat caggcgttta ttgccggctg tatgatggca ccgacagcgt taggttccat
     1801 tattgcaaaa tcgatggtta cccaagtctt acgtcgtctg ggctatcgcc atacattagt
     1861 ggggatcacg gtgattattg ggctaatgat cgctcagttc tctttgcaat caccggcaat
     1921 ggcgatatgg atgctgatct tgccgttgtt tatattaggg atggctatgt cgacgcaatt
     1981 taccgcgatg aataccatca cacttgccga tctgaccgat gacaacgcca gcagcggtaa
     2041 cagtgttctg gcggtcacgc agcaactgtc tatcagttta ggcgttgctg taagtgcggc
     2101 cgtccttcgc gtttatgaag ggatggaagg cacaacgact gtcgaacaat tccactatac
     2161 gtttatcacg atgggcatta ttactgttgc ttcagcagca atgttcatgc ttctgaaaac
     2221 aaccgatggt aataatttga tcaaaagaca gcgtaaatct aagccgaacc gcgttccatc
     2281 agaatcggag taagttgtaa tcgttgttgc tgaagggtcg gttgggttat ccgatggatg
     2341 agtacatcaa tcgccagctc ccctagttca tctttcggtt ggtggatagt ggttaatggt
     2401 ggcgtcataa agcttgccag ttcgatatcg tcatagccaa tcaccgcaat atcctgcgga
     2461 acttgtaact ctgcctgata cagcgcctgg taaacgccaa cagccatagc gtcatttccg
     2521 gtaaagacgg cctgaggacg cagcggatgt aataacagtt ggcgcatagc gtcaaacccg
     2581 ccgttaaatt caaaatcacc agtgacttca tagccatcag gaatgttgag acccgcacgt
     2641 ttcatcgccg cccgataacc ttccaaccgc aggcgtgccg gagttttatc cagcgggccg
     2701 gtaatacagg cgatacgggt atgaccttta tcgatcagat attgcgttgc taagtctccg
     2761 cccagcaacg agttatcctg aataagatcg ctgtcgccat cgaacggagc ccagtccatc
     2821 atcacagtag gcactgtcgg ataacgttgc atgatttcac gcgaaggttg atgcgtttcg
     2881 gtgcacagta acagcaagcc atcaacgcgt ttttgcatca gcgtttccag attgcgattc
     2941 atccgctgtt catcgccttc ggtattgcaa aggacaagac tataaccgcg ttcgaagcaa
     3001 ctgcgttcaa cgccacgcac cagttcagaa tagaaaggat tggtactggc agtgatcaac
     3061 atgccaatgg tatgtgtttg attgagtttg aggctacgcg ccagggctga tggcgcgtaa
     3121 ttgagttctt taatcgccgc ttcaactttg gcggtaatcg cttcactgac gaagcgatct
     3181 ttattgataa cgtgagaaac tgttgaggta gaaacgcccg ccaggcgggc aacatctttc
     3241 attgtagcca agcgtcacct ctgcctgtct aaaaatgcgt cgatctcttc acgccacggt
     3301 acggaaggtt gtgcgccttt acgtgttacg gcaatcgcag cggcagcatg agcaaaccga
     3361 atcgcctctg gcaatggttt ttcttccagc aacgccgtga ttaacgcgcc gttaaaggta
     3421 tctccggcag caatggtatc gacagcctgc acccggaatc caggaacgcg ctgaccttca
     3481 ccattcacgc tagcccatac accacgactt cctaaagtaa tcagtacagt acggatacct
     3541 ttttcatgca gtacctgcgc cgccttcgct gcatcttcat cattttcaac acgaataccg
     3601 gtgagctttt ctgcttccgt ttcgtttggc gtaataatgt ccaccagtgc cagcagttcg
     3661 tcaggaagtt cgcgagccgg agccgggtta agcgcaacga tagtcttatt ttgatgggcg
     3721 attttcgccg ctgccatcac actttcgagt ggtgattcca gctgcattaa taatgctgac
     3781 gcgttggcaa tacgctcacg ttgcgcttcc accagtgccg gggaaagggc agcattagcg
     3841 ccggcatgaa taccgatgac attctcacct tcgccattaa caaaaatcag cgccacacct
     3901 gttgattcgc ctttgatcac gctgaccgga gaaatatcga tgttatcagt ggcgagctgc
     3961 tggcgaacgc tctcaccaat gctgtcatcg ccagtgcagg caataaacgc gatattcgca
     4021 ccgctacgcc cagcagccac agcctgatta gcgcctttgc cgccaaatgc aacctgatag
     4081 tggttaccgg ttacggtttc gcctggggta ggaaaagatt gaagattaag aatgtggtca
     4141 gcattaatgc tgccaagaac aacgaggctg cctgcgtttt gcatattcgg gatgtccata
     4201 aaatgcgcca ccgtgttagg gtggcgcgtg ccctgctttt ctttatatta cacgttcatc
     4261 tgtcgatgcc gtatttatgt caccatcagg tcatacaacc tgattaaaac tactgcttaa
     4321 caaccagttt cagatcaacc ggatacttag cctgaacttt ctcacctttc agtactttat
     4381 cggcggtttc gacgcctttc gcgccaatct gatcgggtag ctgagcgata gtcgctgcta
     4441 gtttgccatc attcaccgct ttttcgccat ccggtgtacc gtcaaatccg acgaccatca
     4501 catccgattt accggcagtt tgcagtgcgc gcagcgcgcc cagcgccatt tcatcattct
     4561 gcgcgaatac agcctgaaca tccggatgtg cggtcaacag gttctgcatt acgttcaaac
     4621 ctttagtgcg atcaaaatct gctggctggc tggcaagaac attaaacttg tgagcagcaa
     4681 cagcctgctg gaagccttcg ccacgttcac gggctgcgga tgtaccggca atgccttgca
     4741 gctcgataac tttggcacct tcacccgctt tcttcgcgat gtaatcaccg gcgattttgc
     4801 cgcccagtac gttatcagaa gcaatgtggc tcaccacttc acctttcgtt gcctggcggt
     4861 caagagtgat aaccgggatg ttcgcctggt tagccatctt cacagcatta cccactgcgt
     4921 cggagtcggt cgggttaatc agcagaattt tggtgccgcg aacggttaag tcctgcacgt
     4981 tcgccagctc tttcgccggg ttattctggg agtccagcac caccaggtta tagccaagtt
     5041 tatccgcctc tttctgcgcg ccatctttca gcgatacaaa gaacgggttg ttaagcgtgg
     5101 agaccaccag cgcgatggtg tcttttgcca tcgcattcgc actgacggtg gcgcttagcg
     5161 caacagcgga aaccagggta gccagttttt tcatgttcat attcaagatg tcctgtagtc
     5221 gttattactg ctttttgttg tctaccagca ccgccagcaa aatcaccacc gctttgacga
     5281 tcatctggta ataggaggaa acacctaaca aattcaatcc attattaagg aagccaagaa
     5341 ttaatgcgcc gatcaacgtc ccaacaatgc gaccttttcc gcccgccaga ctcgtaccgc
     5401 ccagaaccac cgcagcaatg gcatccagct catagccagt ccccgccgta ggttgtgcgg
     5461 aggagagacg cgccacttca atgatcccgg ccagcgatgc cagcagacca caaagagaat
     5521 agacgatgat tttgatttta ttgacgttga taccagaaag gcgcgttgcc gcttcgttgc
     5581 cgcccagcgc gtagatgtaa cgccccagac gcgtgtgatg cagcatgtac caggccgcga
     5641 ggaagacaat tcccatgatc cagactggcg tcggtacacc cagcggacga ccaataccaa
     5701 accagccaaa cagatcggcg ttctcagtaa agccagtatt cactgggcta ccgttggtat
     5761 aaaccatggt cacgccgcgc agtaaaagca tcataaccag cgtagcgata aaggcctgga
     5821 cgcgaccttt cgctacaatc accccggtta ccgcaccaat tgcggcacct aacgcgagag
     5881 cggcagcgac agccaccagc gcattgactt caatgccaac gatagatgca gcaactgcgc
     5941 cggtcagcgc caatagagaa cctaccgaca agtcgatgcc cgacgtcagg atcaccagcg
     6001 tcatcccgac tgccataatg gcgttcaccg aggtttgctg gagaatattg aataagttat
     6061 tgatggtgaa aaagttcggg cttaacgtcg agacaatcgc gatcagcacc agcagagcga
     6121 taagcgattt ctgctccatc agccacgctt tcgtgaaata acggcgacca gagacagtct
     6181 gggttgtcat ttttttactc ctgattcacg cgattaagct tgcccacagc ggcagccatt
     6241 aacacttcct gggtggcctg ctcacgagta aattccccgc tgagatgccc ttcatgcatg
     6301 acgatgatgc gatcgctcat gcctaatact tctggcatct ccgatgacac cagaatgatg
     6361 ctcaagccat cggctttgaa ctggttaatc agttgataga tctctttttt cgcgccgaca
     6421 tctacgccac gggtaggctc atcgaggatc aacactttgg ggcgtgtcat cagaccacgg
     6481 gcaatcgcca ctttttgctg attgccaccg gagagcagac caattgcctg ttccatcgac
     6541 ggcgttttca cattaaacag acgaatgaaa tcacttactg cctgctgttc atcggcatgc
     6601 ttcaaactgc cgccagcgcg gctgaagtag cgcagcgctg tcagcgacat gttctctttt
     6661 actgacatgc ccagcactaa accgtcacgt ttacggtctt cggagatata cacaatgccg
     6721 tttgccaggc catcctgcgg tgaacgggta acgacttcat gcccatccag ggtgacgtaa
     6781 ccgctggtgc gcggcagtgc gccgtagagc actttcatca gttcggtacg acctgcgccc
     6841 atcaaaccag agacgccaag aatttcgcct ttgcgtaaag taaaagagac atcgttaacg
     6901 ccaggtccgc agagattatc gactttcagg cggatatctc ccggcgcttt gtccaggtgc
     6961 ggatattgat cttccagctt gcgacccacc atcatctcaa tcagcgaatc ttcggtcagt
     7021 gatgctactt cacgctcagc aataaattgc ccatcacgaa aaacggtaac gtcatcgcaa
     7081 atctcgaaga tttctttcat gcggtgggag atatagacaa taccgcggcc ttgcgatttc
     7141 agctcgcgga tgacgcggaa caaggattcg gtttcggtat cggtcagcgc atcggtcggt
     7201 tcatccataa tgatgacttt cgactcaaag ctcagcactt tggcaatttc aaccatttgc
     7261 tggtcaccga tggaaagatc gcccaccagc ttgtcgcttt taaagcgcag gttaagttta
     7321 gccagcaatt tatccgcttc ggcatacatg gttttccagt caattttgcc aaagcgatta
     7381 acaaactcac gaccgaggaa aatgttttcg gcaatggtca actgcgggat caggttcagt
     7441 tcctgatgga taatcccaat cccggcttcc tgggaagatt tcggcccggt aaacgtcgtt
     7501 tctttcccca gccataaaag cgtaccggca tcgcgagtat agatacccgt aagcactttc
     7561 atcatggtgg atttacccgc accgttttcg cccaccagcg ccatcacgcg gccaggatag
     7621 acatttaacg ctgcgcccga gagggctttt acgcccggga aggctttatc gatgccttta
     7681 agctgaagta atgcttccat gacggcctca gaacgtcacg ccagcacaga gaatgatatt
     7741 cgcatacgga gaacattctc cgctgcgaat taccgcctga ctttctgcgg tttgttgttt
     7801 gaattgttca tgcgtggtgt aacgaatttc aatggtattt ccctggtgtt tttgcagctg
     7861 ctcaaggtga gtgagcaacg tttcgtggag atgcggatta tgttgtttga tctcttccgc
     7921 gataatggct gcctcgacct gcatttcatt tgtgacgacg cccagcacct gcataaaaga
     7981 aggtacgccc tgggttaatg ccatatcgat acgcgttgta cttttgggga tgggtaaacc
     8041 agcatcacac accaccagcg tatcggtatg tcccagacgg gagatcaccg atgaaatatc
     8101 agaattaaga acggtgcctt ttttcatttt tttctccatc agcgaaacgt ttcgctgacc
     8161 cgcagtttaa tcctgactga gttgggaaaa ccatcgtgac gttacggaaa tgtgatcaac
     8221 ctcgaaacgt tttacatggt aattaaccat gaaaacagaa acgccccctt aaggagaaag
     8281 gaggcgtctg gcgttagatt tcgacctgag tacccagttc gataaccctg tttggcggga
     8341 tttcaaattg atccggcgca cgcagcgcat tacgttgcag cagcaagtac agcttgccgc
     8401 gcagacgcaa ataccacggg cgtttgccga ggatcaacga ctcatgcgac ataaagaagg
     8461 aggtttccat catccggcaa cttaatcctt ccagaccgca gcggtggaaa acttcttcca
     8521 cgtttggcgt ttctcgccaa ccataacttg ccaccacgcg ccagaaagtg ggcgacagtt
     8581 gttcaatctg tacccgacgg acgttatgga catatggcgc gtcttcggtg cgcagagtta
     8641 acagaatcac ccgctcatgc aataccttgt tatgtttaag gttatgcatc agcgcaaagg
     8701 gaatgacgtt gattgcacgt gacatataca ccgcggtccc gggcacgcga acgggcggtg
     8761 atttctccag cgaagcaatc atcgcctcca gagagttacc atgttcatgc atccgccgca
     8821 gcaaacggaa acgttcgctc ttccaggtgg tcatcacgat aaacatcacc gtaccgaggc
     8881 tcaatggcaa ccagccgccg gagagcagtt tatcgaggtt agcggtgaac aatggaatat
     8941 cgacacaaag gaaagcaatc aggatcagcg caacaaaata cttattccag tgccagttct
     9001 gacgtgccac ggtagtcgag agaatagacg tcagcaccat ggttccggtc accgcaatcc
     9061 cgtacgccgc cgccaggttg ctggagtgct caaagctgac aatcacaatc acgaccgcga
     9121 catagagcat ccagttcaca aagggaatat agatttgccc tgactccatt tcggaggtgt
     9181 gaataatgcg catcggcgac aaatatccca gacgtaccgc ctgacgcgtc aatgagaaga
     9241 cgccagagat aacagcctgc gaggcaatta ccgtcgccag tgcggcgatg atcagcagcg
     9301 ggatcagcgc ccagtccggt gccaacagga agaacgggtt cttaatcgct tccgggttct
     9361 ttaacaacaa cgctccctga ccgaagtaat taagggtcaa ggaaggtaat acgacggtga
     9421 accacgccag gcgaataggg aacttaccaa agtgccccat atcagcatac agcgcctcga
     9481 cccccgtaat cgacagcacc actgccccta atgcaataaa agaaaccgtt ttgtattcaa
     9541 ggaagaaatg caccgcccac atcggattca gcgcatgcag cacttccggg ttagcaataa
     9601 tgctacgtaa ccccagtcct gccagaatca aaaaccaggt cagcatgatc ggcgcaaaca
     9661 gcttaccgac catagccgtg ccatgctttt gaatcataaa taataaagtg agaacgataa
     9721 ttgagagggg aactatccag gtatccagct gcggggcgac gatttccaga ccttcaatgg
     9781 cggacatcac cgaaatagcg ggtgttatga cgacttcacc atagaaaaag ctgccgccga
     9841 ttagccccat aatcaccagc attgatgtgg ttcgcgccga cgtattacgc ccggcaagcg
     9901 acatcaacgt caggatcccc ccttcaccgg cgttatctgc ccgcatcacg aaggtgagat
     9961 atttaataga aaccacaaag attagcagcc agaagatcag cgataaaaag ccaaacacgg
    10021 catcgcgctc aacgccaaaa ccaaactggc cggacaaaca ttcacgaagt gtatataacg
    10081 ggctggtacc aatatcgccg tagacaactc caatcgccgc gagggtaatc gcgggcaatg
    10141 attgcttatt atcagtgctc atagactagt ctttcgttga aatatgaaat gtgtgcttag
    10201 tcccttggcc cacaaaaaag cgcacagtat gcacgattaa cggcaaaatc gtactcctaa
    10261 atgcgggcac attaacctgg ctcaaagaaa aatacccccc ctttgagact attttagcca
    10321 ccagcaagaa acgtctatac tcgcaattta cgcagaactt ttgacgaaag gacgccactt
    10381 cattatggct caccctcatt tattagcgga aagaatttcc cgcctgagca gttcgttgga
    10441 aaaggggctt tatgaacgta gccacgccat ccgcttgtgt ttattagcgg cattaagtgg
    10501 tgaaagtgtg ttcctccttg gcccgccagg tattgccaaa agtttgatcg cccggcgctt
    10561 aaaattcgcc tttcagaatg cccgcgcgtt tgaatatctg atgactcgct tctccacgcc
    10621 ggaagaggtt tttggtcccc tttctattca ggcgctaaaa gatgaagggc gctatgaacg
    10681 tttaaccagc ggttacctgc cggaagcaga aatcgtcttt ctggatgaga tctggaaagc
    10741 gggtccggca attcttaata ccctgctcac cgccattaac gagcgccagt tccgcaacgg
    10801 tgcacacgta gaaaaaatcc cgatgcgcct gctggtggcg gcctccaacg agctgccgga
    10861 agcagacagc agtctggaag cgttatatga ccgcatgctg attcgtctgt ggttagataa
    10921 agtgcaggat aaagcgaatt tccgctccat gctgaccagt caacaggatg aaaacgacaa
    10981 tccggttcct gcctccttgc aggtcacaga tgaagaatat gaacgctggc agaaagagat
    11041 tggtgaaatt acgctgcccg atcatgtatt tgagctgatt tttatgctgc gccagcaact
    11101 ggataaatta ccggatgcgc cttatgtctc ggatcgtcgc tggaaaaaag cgattcgatt
    11161 attgcaggcc agcgcctttt ttagcggtcg cagtgctgtt gccccggttg atctcatttt
    11221 gctgaaagat tgcctgtggt atgacgcgca aagcctgaat ttgatacaac aacaaattga
    11281 tgtattgatg accggtcatg cctggcaaca gcaaggaatg ttgacccgcc tgggcgcgat
    11341 tgtgcaacgt cacctgcaac tccagcagca acaaagcgat aaaacagcct taacggtaat
    11401 tcgtctgggc ggcattttca gccgtcgtca gcagtatcaa cttcctgtta acgttactgc
    11461 ttccactctg actctgctgc tgcaaaaacc gttaaaactg catgatatgg aagtggttca
    11521 tatctccttt gagcgtaacg cgctggaaca gtggctgagt aaaggtggtg aaattcgcgg
    11581 caaactgaac ggtatcggct ttgcccagaa attgaatctg gaagtggata gcgcccaaca
    11641 tcttgttgta cgcgatgtaa gtttacaagg cagtacgctg gcacttcccg gttcatcggc
    11701 tgaaggtctg ccaggtgaaa taaaacaaca actggaagag cttgaaagcg actggcgcaa
    11761 gcaacacgct ttattcagcg aacagcaaaa atgtctgttt atccctggcg actggttagg
    11821 tcgcattgaa gccagcctac aggatgtcgg cgcccagatt cgccaggcac aacaatgcta
    11881 acgctggata cgcttaatgt gatgctggcc gtcagcgaag agggattgat cgaagagatg
    11941 atcatcgcgc tgctggcctc accgcagctg gcggtcttct ttgaaaaatt cccacgactg
    12001 aaggcggcaa tcactgatga tgttccccgc tggcgtgagg cgctgcgcag tcggctgaaa
    12061 gatgcccgag tcccgccgga actcaccgaa gaggtgatgt gctatcagca aagccagctc
    12121 ctctccacgc cgcagtttat tgtgcagcta ccacagatcc tggacttact gcatcgtctg
    12181 aattctccat gggcagaaca agcccgacag ttggttgatg ctaacagtac gatcacttca
    12241 gcgttacaca cgctttttct ccagcgctgg cgtttaagtc tgatcgtgca agcaacgacg
    12301 ctaaatcagc agctattaga agaagaacgc gaacaactgt tgagtgaagt tcaggaacgc
    12361 atgacgctga gcgggcaact tgaaccgatt ctcgcagata acaataccgc agctggtcgt
    12421 ctgtgggata tgagcgccgg tcagcttaaa cgcggcgact atcagttgat tgtgaaatac
    12481 ggtgaatttc ttaacgaaca gccggaactg aaacgcctgg cagaacagtt ggggcgttca
    12541 cgggaagcca aatcaatacc gcgcaacgat gcgcagatgg aaaccttccg caccatggtg
    12601 cgcgaaccgg cgacggttcc tgagcaggtt gatggtctgc aacaaagcga tgatatttta
    12661 cgtctcctgc cgccagaact ggcgacactg gggataacgg aactggagta tgagttttac
    12721 cgtcggctgg tggaaaaaca gttgctcacc tatcgcctgc acggtgagtc gtggcgtgaa
    12781 aaagtgatcg aacgcccggt ggtacataaa gattacgacg aacagccgcg cggaccgttt
    12841 attgtctgtg tggatacttc cggctcaatg ggcggcttta atgaacagtg tgcgaaagcg
    12901 ttctgcatgg ccttgatgcg cattgctctc gctgaaaacc ggcgctgcta tattatgtta
    12961 ttttccaccg agatcgtccg ttatgagctt tcaggcccac aaggcatcga acaagcaatc
    13021 cgttttttaa gccagcagtt tcgtggcggc accgatcttg ccagttgttt tcgcgccatt
    13081 atggaacgct tgcaaagcag ggaatggttt gatgccgatg cggtggtgat ttctgatttt
    13141 atcgcccagc ggttgcctga cgacgtgacg agtaaagtga aagagctaca gcgcgtacat
    13201 cagcatcgct ttcatgccgt ggcgatgtct gcacatggca aacccggcat catgcgcatt
    13261 ttcgatcata tctggcgctt tgataccggg atgcgaagcc gcctgctcag acgctggcgg
    13321 cgataaatta ttacagcaga gaagggacgc tctcgcgaac agctgccggc catacaccac
    13381 actgaacctg gccgatatgt ggcagttgca gcagcagcat ggtcaaacga gactgcccga
    13441 taccgccgcc gatggtctgc ggcatttcac cgcgcagcag cgcctgatgc cactccagct
    13501 ccaggcgatc ttcatcaccg gtcagcgcca gctgatgttt caacgtatcg gcatccacgc
    13561 ggatccccat ggaagaaagc tcaaacgcat cttccagtac cgggttccat accagaatat
    13621 cgccgttcag acccgcatgg cccagctctg acggggtgct ccagtcatca taatccggtg
    13681 cgcgcacgtc gtgacgatga ccatcactca acttgccgcc aatcccgacg aggaataccg
    13741 cgccaagatc tttcgcaatc gcccgctcac gccctttggc atcgagatcc ggataacgag
    13801 acagtaactc ctggctgtgt acgaagtgga tctgatccgg caggaacggt gccaggccaa
    13861 actcttcgct aaccgcagct tcggttgctt taattcccgc ccagatcgcc tctaccgtgc
    13921 ttttcagagt cgagaattga cgctcaccgt cgcccattac gcgttcccag tcccactggt
    13981 caacatagac cgagtgcaac ggagaaagac ggtcttcatc ggggcgaagg gctttcatgt
    14041 gcgtgtacag cccttcgccc gcgctgaagt cgtgttgccc taatgtctga cgtttccact
    14101 tcgccagtga atgaaccact tcgaactggg catcaggcag agctttcact tttacctgca
    14161 ccgctttttc acagcccgac aagttatcct gcgtgccatc ccccacacgg ctaagaatcg
    14221 gcgcctggac ttcgatcagc cccagacgtt cttccagttg acgggaaaag tgagatttca
    14281 cgaagctaat ttgacgttgt ttggcaatgt aagcggtttt cattttttat actcctgcgt
    14341 cctgttgctt atgattaagc aacaaaaacc gacacgcatg caataatcat tcaataaaaa
    14401 gcctaactca cttttgattc attaaaaaag aaggctaaaa tagaatgaat catcaatccg
    14461 cataagaaaa tcctatggaa aattatctaa tcgacaatct ggaccgtggc atcctggaag
    14521 cattaatggg caatgcgcgc accgcttacg ccgaactggc gaaacaattt ggcgtcagtc
    14581 cggggacgat tcacgttcga gtagagaaaa tgaagcaggc ggggatcatt accggggcgc
    14641 gtattgatgt cagcccgaag cagctcggtt atgacgtagg ctgctttatc ggcattatat
    14701 taaagagcgc caaagactat ccttccgcgc tggcgaaact ggaaagcctt gatgaagtca
    14761 ctgaagccta ttacaccacc ggccactaca gcatctttat aaaagtgatg tgccgttcga
    14821 tcgacgctct ccagcatgta cttatcaaca agatccaaac aattgatgaa attcagtcca
    14881 ccgagacatt gatcgtcctg caaaacccga tcatgcgtac catcaagccc tgatcggctt
    14941 ttttaatccc atacttttcc acaggtagat cccaacgcgt tcacagcgta caatacgcca
    15001 ctcttaataa aggtggcggt ttatggcaga tatcactctt atcagcggca gcaccctcgg
    15061 cggtgccgaa tatgtagcag aacacctggc tgaaaagctg gaagaggcgg gttttaccac
    15121 cgaaacgctg cacggtccgc tgttggaaga tttatctgcc tctgggatct ggctggttat
    15181 cagctcaacc cacggtgccg gagatattcc ggacaacctt tctcctttct atgaagcatt
    15241 gcaggaacag aagcccgatc tttctgcggt ccgctttggc gcaatcggta ttggtagtcg
    15301 tgaatatgac accttttgtg gggctatcga taaactcgag gcagaactca aaaattccgg
    15361 tgcaaaacag acaggcgaaa cactgaagat caacattctt gatcacgaca ttccggaaga
    15421 tccggcagaa gaatggctgg gatcgtggat taatttactc aaataagtat acagatcgtg
    15481 cgatctactg tggataactc tgtcaggaag cttggatcaa ccggtagtta tccaaagaac
    15541 aaccgttgtt cagtttttga gttgtgtata acccctcatt ctgatcccag cttatacggt
    15601 ccaggatcac cgatcattca cagttaatga tcctttccag gttgttgatc ttaaaagccg
    15661 gatccttgtt atccacaggg cagtgcgatc ctaataagag atcacaatag aacagatctc
    15721 taaataaata gatcttcttt ttaataccca ggatcccagg tctttctcaa gtcgacaaag
    15781 ttgagtagaa tccacggccc gggcttcaat ccattttcat accgcgttat gcgaggcaat
    15841 caccatgttt tatccggatc cttttgacgt catcatcatt ggcgggggtc atgcaggcac
    15901 cgaggccgcg atggccgcgg cgcgtatggg tcaacagact ctgcttttga cacacaatat
    15961 cgacactctg gggcagatga gctgcaaccc ggcgatcggc ggtattggga agggacatct
    16021 ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa gcgatcgatc aggcgggtat
    16081 ccagtttagg atactaaacg caagcaaggg accggcggtt cgcgctaccc gagctcaggc
    16141 ggatcgtgtg ctctaccgtc aggcggtacg tacggcgctg gagaaccaac cgaacctgat
    16201 gatcttccag caggcggttg aagatcttat tgtcgaaaac gatcgcgtgg tcggtgctgt
    16261 tacccaaatg ggactgaagt tccgtgccaa agccgtcgtg cttaccgttg ggacgttcct
    16321 cgacggtaaa attcatatcg gtctggataa ctacagcggt ggtcgtgctg gtgatccgcc
    16381 gtccattccg ctctctcgcc gtttgcgtga actgccgctg cgcgttggtc gtctgaaaac
    16441 cgggacaccg ccgcgtattg atgcgcgtac tatcgacttc agcgtgctgg cgcaacagca
    16501 tggcgataac ccaatgccgg tattctcgtt tatgggtaat gcgtcccagc atccacagca
    16561 ggtgccgtgt tatatcaccc ataccaacga gaaaacccat gatgtgatcc gcagtaacct
    16621 cgatcgtagc ccaatgtatg caggggtgat cgaaggtgtc ggcccacgct actgcccgtc
    16681 gatcgaagac aaagtcatgc gcttcgccga cagaaatcag catcagatct tccttgaacc
    16741 ggaagggctg acctcaaacg aaatttatcc gaacggtatc tccaccagcc tgccgttcga
    16801 tgtgcagatg caaatcgtcc gctccatgca ggggatggaa aacgcgaaga tcgtgcgtcc
    16861 gggttatgcc attgagtatg acttcttcga tccacgcgac ctgaaaccga cgctggagag
    16921 caagtttatc caggggctgt tctttgctgg tcagattaac ggcactaccg gttacgaaga
    16981 agccgctgcg caaggtttgc tggccggtct taacgctgcc cgtctgtctg ctgacaaaga
    17041 aggttgggct ccggcgcgtt ctcaggcgta tctcggcgta ctggttgatg acctgtgcac
    17101 tttaggaacc aaagaaccgt atcgtatgtt tacctcgcgc gcagaatatc gtctgatgct
    17161 gcgcgaagat aatgcggatc tgcgtttgac tgaaattggt cgtgaactgg gcctggtgga
    17221 tgacgaacgt tgggcgcgct ttaacgagaa acttgagaat atcgagcgtg agcgtcagcg
    17281 tctgaaatcg acctgggtaa ccccgtcggc ggaagctgca gccgaagtga atgctcacct
    17341 gactgcgcca ctttcccgtg aagccagtgg tgaagatctg ctgcgtcgtc cggaaatgac
    17401 ttatgaaaaa ttaaccacgc tgacgccgtt tgcccctgcg ttgacagacg aacaggcggc
    17461 ggaacaggtt gagattcagg ttaaatacga aggttatatc gcgcgccagc aagatgagat
    17521 cgaaaagcag ctgcgtaacg agaacaccct gctaccagcg acgctggatt accgccaggt
    17581 atccggtctt tctaacgaag tgatcgccaa acttaacgat cacaaaccgg cctctatcgg
    17641 ccaggcttcg cgtatttctg gcgtcacgcc tgcggccatc tccattctgc tggtgtggct
    17701 gaaaaaacag ggtatgctgc gtcgtagcgc ataacgcatt aaaaatgcct ggtaagcacc
    17761 cgcttaccgg gcaacgcatc aagaacaggt aatcaccgtg ctcaacaaac tctccttact
    17821 gctgaaagac gcaggtattt cgcttaccga tcaccagaaa aaccagctta ttgcctatgt
    17881 gaatatgctg cataaatgga ataaagcgta caacctgact tcggtccgcg atcctaatga
    17941 gatgctggta cgccatattc tcgatagcat tgtggtggca ccgtatctgc aaggtgaacg
    18001 gtttatcgat gtcggcaccg gtccaggact gcctggcatt ccactctcta tcgtgcgtcc
    18061 tgaagcccat ttcactctgt tggatagcct tggtaaacgc gtgcgtttcc ttcgtcaggt
    18121 gcaacatgag cttaaactgg agaatattga gccagtacag agcagggtag aagagtttcc
    18181 ttcagagccg ccatttgatg gcgtaattag ccgcgctttt gcctctctga acgatatggt
    18241 gagctggtgt caccatcttc ctggtgagca aggccgtttc tacgcgctga aaggccaaat
    18301 gccggaagat gaaatcgctt tgttgcccga agaatatcag gtcgaatcag tggttaaact
    18361 tcacgttccg gccctggatg gcgaacgtca tctggtggtg attaaagcaa ataaaattta
    18421 atttttatca aaaaaatcat aaaaaattga ccggttagac tgttaacaac aaccaggttt
    18481 tctactgata taactggtta catttaacgc cacgttcact cttttgcatc aacaagataa
    18541 cgtggctttt tttggtaagc agaaaataag tcataagtga aaatatcagt ctgctaaaaa
    18601 tcggcgctaa gaatcatcat tggctgttaa aaca
//
LOCUS       DAZKJR010000102        18116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKJR010000102 DAZKJR010000000
VERSION     DAZKJR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.103"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            215..781
                     /locus_tag="ACHOWS_003552"
     CDS             215..781
                     /locus_tag="ACHOWS_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0049076.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMTTTFTGNPYSGN
                     SKNYSLSNMDNGTEIPNVSLVIGDQRGTGYDLGAGIDQDIAMDSSTGKGKQKQTLNFK
                     AWLVGETAAVTPTPAPFETLTTFQITYL"
     gene            867..3506
                     /locus_tag="ACHOWS_003553"
     CDS             867..3506
                     /locus_tag="ACHOWS_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFQ0049077.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNVLADDTIQFDARFLELKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNHPLPDEYDIYWYVAENDPNKSYACLPPELI
                     AQFGFKDDFAKSLQWGHDGQCLKTDQIGGMEIKGDLSQSALLVSVPQAYLEYTDDDWD
                     PPSRWDEGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNMRGYAPDISGVARTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALFGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDTDRARTGNDKEMY
                     TVTYNQNFRDARVSVYLNYSHHTYWDREDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSSQASLYHRIDDASHYTLSAGTS
                     ENHTSLDGYYSHDSTLAKVDLSANYHEGQYTSAGVSLQGGVTLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATQ
                     SVVQATLTEGAIGYRKFAVISGEKAMVVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSAPVITHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            3526..4275
                     /locus_tag="ACHOWS_003554"
     CDS             3526..4275
                     /locus_tag="ACHOWS_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFQ0049078.1"
                     /translation="MTKHLSVKTLATVLSLLAATVSFSALASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKQPYLAQSWIEDEKGVKISSPLAVLPPVQRIDAMMNGQVKIQGLPDIH
                     KLPADRESIFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALEKASMQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPADGFSPIVMSPKTSAPLKVKMASVPVL
                     TYVNDYGARMPLFFTCSGNSCQVDEELSRKG"
     gene            4292..4798
                     /locus_tag="ACHOWS_003555"
     CDS             4292..4798
                     /locus_tag="ACHOWS_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0049079.1"
                     /translation="MKRSCSVWAGTWLLMCCIGSDFAVAGSKSVNLSLRVLVDAPPPC
                     TVNGATVEFGNVFINKIDGVGYKQPIDYSLVCNNLAMDDLRLQMRATTVVINGETVIS
                     TGISGFGIRVQKSSDHSTLDLTSGSWLPFNFSSGVPALEAVPVKQSGTTLSAAEFNAS
                     ATIVVDYQ"
     gene            4795..5265
                     /locus_tag="ACHOWS_003556"
     CDS             4795..5265
                     /locus_tag="ACHOWS_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0049080.1"
                     /translation="MKRISLLMLWGFSSMALSNVSFHGYLVQPPNCTISNGKTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMKLSWSGTQSDFDDAAVSTDISGLA
                     IRLKQAGQSFKLNTALVVNETALPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            5262..5786
                     /locus_tag="ACHOWS_003557"
     CDS             5262..5786
                     /locus_tag="ACHOWS_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0049081.1"
                     /translation="MKKKRALVFICSLMLLYSVTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVHFPTIAASDLMNLGQSERVPLVFQLKDCKSSTLFSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGAAVPVNSTTGVTFPLNQGNNSLNFNAWLQAKGGRDVTS
                     GDFSATATATFEYF"
     gene            5788..6630
                     /locus_tag="ACHOWS_003558"
     CDS             5788..6630
                     /locus_tag="ACHOWS_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFQ0049082.1"
                     /translation="MKKWTMIFASLVLLVMSASVPKSYAGDKLTSASFDSVKIYYGKG
                     KMSIHNTGGLYVTVLTPNEAAYGVYNDAIRKNEQASVISWTGSGPAPVLILTDYGNAD
                     SSNCPGINTATFQCAYETFKITVQSDSYGCPWLASFYTITDQPGFGRYTGPTNHATVC
                     PTIPVASYDISWSENYVSHNKALQLQSNGSTITTTLSTYLMEDGKLCDGSVFDSRGAY
                     CRVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVNVNTSSRQPIDSTCRFQYV
                     LNEL"
     gene            complement(6746..7297)
                     /gene="smrB"
                     /locus_tag="ACHOWS_003559"
     CDS             complement(6746..7297)
                     /gene="smrB"
                     /locus_tag="ACHOWS_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFQ0049083.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7463..8395
                     /gene="prmB"
                     /locus_tag="ACHOWS_003560"
     CDS             7463..8395
                     /gene="prmB"
                     /locus_tag="ACHOWS_003560"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0049084.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8430..9515
                     /gene="aroC"
                     /locus_tag="ACHOWS_003561"
     CDS             8430..9515
                     /gene="aroC"
                     /locus_tag="ACHOWS_003561"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0049085.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9518..10342
                     /gene="mepA"
                     /locus_tag="ACHOWS_003562"
     CDS             9518..10342
                     /gene="mepA"
                     /locus_tag="ACHOWS_003562"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFQ0049086.1"
                     /translation="MNKTAIVLLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10342..11151
                     /locus_tag="ACHOWS_003563"
     CDS             10342..11151
                     /locus_tag="ACHOWS_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0049087.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11151..11699
                     /gene="epmC"
                     /locus_tag="ACHOWS_003564"
     CDS             11151..11699
                     /gene="epmC"
                     /locus_tag="ACHOWS_003564"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFQ0049088.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11733..12011
                     /locus_tag="ACHOWS_003565"
     CDS             11733..12011
                     /locus_tag="ACHOWS_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFQ0049089.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQKASQQ"
     gene            complement(12067..14073)
                     /gene="mnmC"
                     /locus_tag="ACHOWS_003566"
     CDS             complement(12067..14073)
                     /gene="mnmC"
                     /locus_tag="ACHOWS_003566"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFQ0049090.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHLHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLKLAQQQGLQIRYQHQLKDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYDVTLVEYASLAEQKDEAVSAPIYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14232..15452
                     /gene="fabB"
                     /locus_tag="ACHOWS_003567"
     CDS             14232..15452
                     /gene="fabB"
                     /locus_tag="ACHOWS_003567"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFQ0049091.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15717..16895
                     /locus_tag="ACHOWS_003568"
     CDS             15717..16895
                     /locus_tag="ACHOWS_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0049092.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16892..17887)
                     /gene="flk"
                     /locus_tag="ACHOWS_003569"
     CDS             complement(16892..17887)
                     /gene="flk"
                     /locus_tag="ACHOWS_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFQ0049093.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
BASE COUNT         4346 a         4524 c         4785 g         4461 t
ORIGIN      
        1 taactttgaa tgttagaaaa tctaaattgc gtcagtcata ttaaaatatc aatatgttcg
       61 gcacgccaaa gaatattata tcaaggaacc tgttttttca ggagctgaaa atctgttcat
      121 tggctgctta aaagtcgctg caaaaattga agaagttctg tgtcaatatt tcagttggct
      181 tatattctaa ttatttatga tttaaggaca aataatgagt aagtttgtaa aagcagcagt
      241 tgccacggca atgctcatgg gggcgctgac ttcagcatca gtcgttgccg caggtaacaa
      301 cggtacagtg agattttacg gcacaattga agactctccg tgctcgattg ttcctgacga
      361 tcataaactg gaagtggata tgggcagcat tggtacaggt tctttaaccg gtggcaagac
      421 taccacacct aaagatttcc agattcgttt acaggattgt aatttcaata ctgaaacgac
      481 aatgaccacg acattcactg gtaatccgta ctccggcaac tcaaaaaact attccctgtc
      541 taacatggat aatggaactg aaattccaaa tgtaagtctg gtgattggcg accaacgtgg
      601 tactggttat gacctggggg caggcatcga tcaggacatc gcgatggaca gttcaacagg
      661 aaaaggcaaa cagaaacaaa cgttgaactt taaagcctgg ttagtaggtg aaactgcagc
      721 ggtaacacca accccagcgc cgtttgaaac gctaacgacg ttccagatta catatctcta
      781 attatgctgt tcccccataa ctgcaatgtt atggggttta taaataaaag ataactaaat
      841 tataataatc atggatggat acgtagatgc ctgaacattc ttttcttcgt ttacgggggc
      901 tttcatggtg gattgctctg gcaatttccg ggagtccttt taatgtactg gcagatgata
      961 ccattcaatt tgatgctcga tttctggagt taaaaggaaa taccaaaatt gatctggggc
     1021 gtttttctca aaaagggtat gttgaaccag ggaaatacaa tttacgtgtt catgtaaata
     1081 atcacccatt acctgatgaa tacgatatct attggtatgt agctgaaaac gatccgaata
     1141 aatcatatgc ctgcttacca cctgaactga tagcccagtt tggcttcaaa gatgattttg
     1201 cgaagagttt gcaatggggt catgatggac aatgtctgaa aactgaccaa ataggaggta
     1261 tggaaattaa gggcgattta agtcaatctg cgctcttagt ttctgtgcca caggcatatc
     1321 tcgaatacac tgacgatgac tgggacccac cttcgcgttg ggatgagggg attcctgggc
     1381 ttattgccga ttacagtatc aacgcacaaa cccgacatga aaatgggggg gatgacacta
     1441 acgacatcag cggtaatggt acggtcggcg ttaacgtggg gccgtggcgc ttacgagccg
     1501 actggcagag cgattaccaa catacccgca gtaacgatga cggcgacact gatgatagcg
     1561 gtacgcaaaa aaactgggag tggagccgtt actacgcctg gcgcgccttg ccgtcattaa
     1621 aagccaaact ttcgctgggt gaagattatc tcaactccga tatttttgat ggcttcagtt
     1681 acattggcgg cagcatcagt accgatgatc aaatgttacc gccaaacatg cgcggctacg
     1741 cacccgatat ttcaggtgtt gcacgcacta ccgctaaagt gaccgtaacc caaatgggcc
     1801 gtgtcattta cgaaacccaa gtgcccgcag gtccattccg cattcaggat attggcgatt
     1861 ccgtatctgg cacgctgcat gtccgcattg aagaacaaaa cggtcaggta caggaatatg
     1921 acgtcagcac ggcgtccatg ccgtttctga cccgccccgg tcaggtgcgt tacaaagtga
     1981 caatggggcg tccacaaaac tgggaccatc aggttgcagg cagcttcttc tcgggcggtg
     2041 aggcgtcatg ggggattgcg aatggctggt cgctctacgg cggggcgttg gccgatgaaa
     2101 actaccagtc ggcagcgtta ggcctcggtc gtgacctcgc gctgttcgga gcgctagcgt
     2161 ttgatgtcac tcactctcgt gttcaactcg acgataacag cgtgtatggc aacaaaacgc
     2221 tggacggcaa ctcttatcgc gtcagctatg ccaaagattt cgatgaactc aacagtcggg
     2281 tgacgtttgc cggatatcgc ttctctgaaa aaaactacat gaccatgagt gagtatctgg
     2341 atgcgaacga caccgaccgt gcgcgcacgg gtaacgacaa ggaaatgtac acggtcactt
     2401 acaaccagaa ctttagggat gcgcgcgttt cggtctatct caattattct caccatacct
     2461 attgggatcg cgaggatcag accaactaca acatgatgct ttctcattat ttcaatttag
     2521 gaagtcttcg caacctgagc gtttctctca ccggttatcg ctacgaatat gataaaagtg
     2581 ccgacaaagg tgtttatctg tccttgagtt tgccatgggg cgacaacagc accatcagtt
     2641 acaacggcaa ttatggcagt ggtgccgaca gcagccaggc gagtttgtat caccgcatcg
     2701 atgacgctag ccactatacg ttgagcgccg gaaccagtga aaatcacacc agtcttgatg
     2761 gttattacag tcatgacagt acgctggcaa aagttgacct gagtgcaaac taccatgagg
     2821 ggcaatacac atctgcgggg gtctccttac agggcggtgt aacactgact gctcacggcg
     2881 gggcgttgca tcgtacgcaa aatatgggag gcacacgtct gctaatcgac gccgatggcg
     2941 ttgctaatgt gccggtcgaa ggtaatggtt ccgccgttta taccaacatg ttcgggaaag
     3001 cggtggtggc agatgttaat gattactacc gtaaccaggc ttatatcgat ttgaacaaac
     3061 taccggaaaa cgccgaagct acccaatcgg tggtccaggc aacgctgacg gaaggcgcta
     3121 ttggctaccg taaatttgcg gtgatcagcg gtgagaaggc gatggtagtg ctgcgcttgc
     3181 aggatggcag ccatccgcca tttggggcgg aagtgaaaaa cgataatcaa caacaagttg
     3241 ggctggttga tgacgaaggt aatgtttatc ttgcaggcgt taagccgggt gaacatatga
     3301 ccgttttctg ggagggagaa tctcactgcg atatcagcct gccagatccg ttaccgaatg
     3361 acctgttcaa tggcttgttg ttgccatgcc agcaaaaagg gggaagtgct cccgtcataa
     3421 cgcatgatat tcaacctgta attcaggagc agacacaaca ggtgacacca atggaaccgc
     3481 cgatgtctgt ttcatcgaac caataacgtg attaaggaat gatccatgac taaacatctc
     3541 tctgtcaaaa cattggcaac ggtactgtcg ttactggctg caacggtaag tttttctgct
     3601 ctggcgtcag taacgcccga tcgtactcgc ctgattttta atgaaagcga taaatccatc
     3661 agcgtcacgc tgcgtaataa cgacccgaaa cagccttatc tggcacaaag ctggatcgaa
     3721 gatgaaaagg gcgtcaagat tagttcgccg ctggcagtat tgccccccgt acagcgcatt
     3781 gatgcaatga tgaatggtca ggtgaaaatt cagggattgc cagatatcca taaattgcct
     3841 gccgatcgtg aatcgatatt ttatttcaat gtgcgtgaaa ttccgccgaa atcgaacaaa
     3901 gccaatacgt tacaaattgc gctgcagaca cgcatcaaac ttttctggcg acctaaagcc
     3961 ctggaaaaag ctagcatgca aaacccatgg cagcataaag tgaccttaac ccgtaacggt
     4021 caggaattta ccgtcaataa ccctactccg tattacgtca ttttcagtaa tgccagcgcg
     4081 caaaaaaatg gcaatccggc agacggtttc tcgccgatag tgatgtcgcc aaaaacctcc
     4141 gcaccgctca aggtgaagat ggctagcgtg ccggtgctga cctatgtcaa cgactatggc
     4201 gcacggatgc cactcttttt cacctgtagc ggcaacagtt gtcaggttga tgaagagctg
     4261 agccgcaaag gttaatgacg gggaagtaag tatgaaaaga tcttgttcag tctgggcggg
     4321 aacatggtta ctgatgtgtt gtattggtag cgactttgca gttgcaggga gtaaatcagt
     4381 aaatttgagt ttacgagtgc tcgttgatgc ccctcctcct tgcacggtta atggtgcgac
     4441 ggttgagttt ggtaatgtat tcatcaataa gatcgatggg gtaggttata aacaacccat
     4501 tgattactcg cttgtttgta acaacctggc aatggatgac ctgcgtttac agatgcgggc
     4561 aacaacggtt gtgatcaacg gtgaaacagt tatcagcaca ggtatttcag ggtttggtat
     4621 tcgggttcag aaaagtagtg atcactcaac actcgactta acgtctggtt catggttgcc
     4681 gtttaatttt tcctcgggcg tccctgcact cgaagcagtg ccggtgaaac agagtggtac
     4741 aaccttatcg gcagcggaat ttaacgccag tgccacaatt gtggtggatt accaatgaaa
     4801 cggatctcac ttcttatgct gtggggcttt agctcgatgg cactctcaaa tgtctctttt
     4861 cacggctatt tagtgcagcc acctaactgc actattagca acggtaaaac cattgaaata
     4921 acctttcagg atgtttttgt tgatgacatt aatggcagta actatgagca aaccgtgccg
     4981 tattccatta cctgtgatac ggcggttcgc gacccatcaa tggaaatgaa attgagctgg
     5041 agcggtacgc agagcgactt tgacgatgcc gcggttagca ccgatatctc tggcctggcg
     5101 attcggctta aacaggcagg gcagagcttt aagctcaata cggcactggt ggtgaatgaa
     5161 acggccttac cggtgttaac cgcagtaccg gtcaaaaaga gcggtgtcga tttgcctgag
     5221 gcagactttg aagcctgggc cacgttacag gtggattatc aatgaagaaa aagcgtgcgt
     5281 tggtttttat ctgtagcctg atgctgttgt acagcgttac tgccccggcg gtcgacaacc
     5341 tgcactttac cggtaatttg ctcggtaaat cctgtacgcc agtgatcaac ggtagcctgt
     5401 tggcggaggt gcactttccc accattgccg ccagcgattt aatgaacctc gggcagtcag
     5461 agcgagtacc gttggtattt caattgaagg attgcaaaag ctccacgctg tttagcgtca
     5521 gggtaacgct gacgggaacg gaagatagcg aactgcccgg atttctggcc tttgacgcct
     5581 catcttctgc atccggagct gggattggta ttgaaacggc ggcaggcgcg gcggtgccag
     5641 tcaacagcac aacgggtgtc acgttcccgc taaaccaggg caataacagt ctcaatttta
     5701 atgcctggct acaggcgaag ggcgggcggg atgtgacgtc cggtgatttc agcgctacgg
     5761 cgacggcaac ctttgagtat ttttaacatg aaaaaatgga caatgatttt tgcgagcctg
     5821 gtgttgctgg tgatgagtgc gagcgttcca aagagctatg ccggggataa actcaccagc
     5881 gcgagtttcg acagtgtgaa gatttattat gggaagggga aaatgtctat tcataacacc
     5941 ggaggtttgt atgtcacggt gttaactccc aacgaggcgg cctatggggt gtacaacgat
     6001 gccataagga agaacgagca agccagcgtt atttcgtgga cggggtcggg accagcgcca
     6061 gtgttgatct taactgacta cggtaatgct gatagctcga attgtcctgg gataaatacg
     6121 gcgacatttc agtgtgcata tgagacgttt aagataaccg ttcagtccga cagttatggt
     6181 tgcccgtggc tcgcttcttt ttatacgatc acagatcaac ccggattcgg gagatatact
     6241 gggccaacga accatgccac agtatgtccg acgatccctg tcgcgtcgta tgacatctcg
     6301 tggagtgaga attacgtcag ccacaataag gcgttacagt tgcaatccaa tggctcgaca
     6361 atcaccacca cgctttcgac ctacctgatg gaagatggca aactctgtga tggcagcgtt
     6421 tttgatagca gaggtgcata ctgccgcgtg gtctcggaac tgttaacctt tacctcctac
     6481 ggttgcgata aatcaacggt aacggtttct cccacacggc atccggtaac cgataaacag
     6541 ctacatgata tcgtggtaaa tgtgaacacc agtagccgac aaccgattga ttctacgtgc
     6601 cgattccagt atgtactgaa tgaattgtag tgacgatatt ttcacaagtc gacggggcca
     6661 tctggccctg tcgtcatttc cggcgtctgt tttatgatat gatggctaag gcgaactttc
     6721 cctctacagc attcgatgcc agcattcagg gcaattcagg cggcagccac tcttccactt
     6781 caatcaacac caacaaagcc gcatcgccac catactcttt tggtgcctgg tgaaatgcca
     6841 ttacatgtgg atgctgcgcc agccacagcg gcgtttgctg cttcaaaata tgcttcccgt
     6901 gaccatgcat cacgcaggca caaaacacat gttcacggcg gcaggcggca atcaacgccc
     6961 ccagttcctg tttggcttgc aactgcgtca gaccgtgcaa atcaagaaac aactccggtg
     7021 aataatcacc gcgccgcagt ttcttcgctt caaaatggct gacatccggg cgaacatatt
     7081 tcaccggacc ttcggtattt aataatggct gaaactcatc ggaaaaataa tggctggcat
     7141 cggcctgctc ctgaatcaac cgcttgaccg gtacttcgct gattttttta cgctgcggtc
     7201 ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc catcaactgg cgaaacagcg
     7261 cctggtcctc ctcgctaagt gttgttttct ttttcattta actgtctcta tttctaattt
     7321 tcctacagtt tacctgactc gtagcacaat cgatcgtttt aaacgtattt ttcctcactc
     7381 accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa actagccgcc gaaattattg
     7441 cgatcatgcc ctggaggaat acgtggataa aattttcgtt gatgaagcag taaatgagct
     7501 gcaaaccatt caggacatgt tgcgctggtc ggtgagccgc ttcagcgcgg cgaatatctg
     7561 gtacggccac ggcaccgata acccgtggga tgaagccgtg caactggtgt tgccttcact
     7621 ctacctgccg ctggatattc cggaagatat gcgcaccgcg cgtctgactt ccagcgaaaa
     7681 acaccgtatt gttgagcgcg tgatccgccg cgtcaacgaa cgcattccgg tggcttacct
     7741 gaccaataaa gcctggttct gtggccatga attttacgtc gatgaacgcg tgctggtgcc
     7801 gcgctcgccg attggtgaac tgatcaacaa taaatttgcc ggactcatca gcaagcaacc
     7861 gcagcacatt ttagatatgt gtaccggtag cggctgcatc gccattgcct gtgcttatgc
     7921 cttcccggaa gcggaagtcg acgcggtaga catctctccc gacgcgctgg cggttgctga
     7981 acagaacatc gaagaacacg gtttgatcca caacgtcatt ccgattcgtt ccgacttgtt
     8041 ccgtgatttg ccgaaagtgc agtacgacct gattgtcacc aacccgccgt atgtcgatgc
     8101 ggaagatatg tccgacctgc caaacgaata ccgccacgag ccggaactcg gtctggcatc
     8161 tggcactgac ggcctgaaac tgacgcgtcg cattcttggt aacgcggcag attacctcgc
     8221 tgatgatggc gtgctgattt gtgaagtggg caacagcatg gtacatctta tggaacaata
     8281 tccggatgtt ccgttcacct ggctggagtt tgataacggc ggcgatggtg tgtttatgct
     8341 cactaaagag cagcttattg ccgcacgaga acatttcgcg atttataaag attaagtaaa
     8401 cacgcaaaca caacaataac ggagccgtga tggctggaaa cacaattgga caactctttc
     8461 gcgtaaccac cttcggcgaa tcgcacgggc tggcgctcgg ctgcatcgtc gatggtgttc
     8521 cgccaggcat tccgctgacg gaagcggact tgcaacatga ccttgatcgt cgtcgacctg
     8581 ggacatcgcg ctataccacc cagcgccgcg agccggatca ggtcaaaatt ctctccggtg
     8641 tttttgaagg tgttactact ggcaccagca tcggattgtt gatcgaaaat accgaccagc
     8701 gttctcagga ttatagtgcg attaaggacg ttttccgtcc aggtcatgcc gattacacct
     8761 atgaacaaaa atacggcctg cgcgattatc gcggcggtgg acgttcttcc gcccgcgaaa
     8821 ccgctatgcg cgttgcagca ggggcgattg ccaaaaaata tctcgccgag aaatttggca
     8881 ttgagattcg cggctgcctg acccagatgg gtgatattcc gctggaaatc aaagactggt
     8941 cgcaggtcga gcaaaatccg tttttctgcc cggacccgga caaaatcgac gcgttagatg
     9001 aactgatgcg cgcgctgaaa aaagagggtg actccatcgg cgcgaaagtc accgtcgttg
     9061 ccagtggcgt acccgccgga cttggcgagc cggtttttga ccgcctggat gccgacatcg
     9121 cccatgcgct aatgagcatt aacgcggtga aaggtgtgga aattggtgat ggttttgacg
     9181 tggtagcgct gcgtggcagc cagaaccgtg acgaaatcac caaagacggt ttccagagca
     9241 accatgcggg cggcattctt ggtggtatca gcagcgggca gcaaatcatt gcccatatgg
     9301 cgctgaaacc gacctcaagc attactgtgc cggggcgcac gattaaccgc tttggcgaag
     9361 aagttgagat gatcaccaaa ggccgtcacg atccgtgtgt tgggatccgc gcggtgccga
     9421 tcgctgaagc gatgctggcg atcgttttaa tggatcacct gttacggcaa cgggcgcaaa
     9481 atgctgatgt gaagactgat attccacgct ggtaaaaatg aacaaaactg cgattgtgct
     9541 gctggccctg cttgccagta gcgccagcct ggcagcgacg ccgtggcaaa aaataaccca
     9601 acctgtgccg ggtagtgcac aatcgatagg cagtttttca aatggctgta tcgtcggtgc
     9661 tgacacgctg ccgatacagt ccgaacatta tcaggtcatg cgtaccgatc agcgtcgcta
     9721 tttcggacac ccggatctgg tgatgtttat ccagcgtctg agtcgccagg tgagcaatct
     9781 gggtatgggt acggtgctga ttggcgatat ggggatgcct gctggtggtc gtttcaacgg
     9841 cggtcacgcc agccaccaga ctggactgga tgtcgatatc ttcctgcaac tgccaaaaac
     9901 acgctggacc tccgcgcagc tcttgcgccc gcaagcgtta gatttagttt cccgcgacgg
     9961 taaacacgtt gtcccgacgc tgtggaagcc ggaaattttc agcttgatta aactcgccgc
    10021 gcaggacaaa gacgtcacgc gcatttttgt taatccggcg attaaacaac aactttgcct
    10081 tgatgcgggc accgatcgcg actggttacg caaagtgcgg ccctggttcc agcatcgcgc
    10141 ccatatgcat gtacgactac gttgtcctgc cgatagcctg gagtgtgaag atcaaccttt
    10201 accgccaccg ggcgatggtt gcggggcaga actgcaaagc tggtttgaac caccaaaacc
    10261 gggaacaaca aagcctgaga agaagacacc gcctccgttg ccgccttcct gccaggcgct
    10321 actggatgag cacgtgatct aatggaaacg tttaatagcc tgtttatggt ttccccgctg
    10381 ctgctgggag ttctcttttt tgtcgccatg ctggcgggat ttatcgactc gattgccggt
    10441 ggcggtgggt tactcaccat tccggcattg atggcggcgg ggatgtctcc ggctaacgcg
    10501 ctggcaacca ataaactgca agcctgcggc ggctctattt ccgccactat ctactttatt
    10561 cgccgcaaag tggttagttt aagcgatcag aaactcaata tcgccatgac ctttgtcggc
    10621 tcaatgagcg gcgcattact ggtgcagtat gttcaggctg atgtcttacg gcagattctg
    10681 cccattctgg tgatttgtat cggcctctat ttcttgctga tgccaaagct gggtgaagaa
    10741 gatcgccagc gccggatgta tggactgcca tttgcgctgg tggcgggggg ctgcgtcggt
    10801 ttttacgatg gattctttgg gccagctgcc ggatcgtttt acgcgctggc cttcgttacg
    10861 ctgtgcggat tcaacctcgc caaagccacg gctcacgcca aattactcaa cgcaacgtca
    10921 aacattggcg gtctgctgct gtttattctc ggcggcaaag tgatttgggc gacgggcttt
    10981 gtgatgctgg tggggcagtt cctcggcgcg cgcatgggtt caagactggt gttgagcaaa
    11041 ggacaaaagc tgatccgccc gatgatcgtt atcgtctcgg cggtgatgag tgcaaaatta
    11101 ctttacgata gccacggaca ggagatcctc cactggttgg ggatgaacta atgaacagta
    11161 cacaccacta tgagcagttg attgagattt ttaatagctg ctttgccgat gaatttaata
    11221 cccgtctgat taaaggcgac gacgaaccga tctatcttcc tgctgatgcg gaagtaccgt
    11281 ataaccgaat cgtctttgct catggctttt atgccagcgc aattcatgag atttcgcact
    11341 ggtgtattgc cggaaaagcg cgccgtgagc aggttgattt cggctactgg tattgcccgg
    11401 acggtcgcga tgcccagacg caaagccagt ttgaagatgt tgaagtgaag ccgcaggcgc
    11461 tggactggtt gttctgcgtg gcggcgggat atccgtttaa tgttagctgc gacaatctgg
    11521 aaggggattt cgaaccggac cgcgtggtgt ttcaacggcg ggtacatgcg caggtaatgg
    11581 attatctggc aaacggcatt cccgaacgtc cggcacgctt cattaaagca ctacaaaatt
    11641 attatcacac gccggaactt acggcggaac agttcccgtg gccggaagcg ctcaactgaa
    11701 gccagcgcca cgttttacaa tgaggaaaga agatgatcgc ggagtttgaa tcacgcattc
    11761 tggcattaat cgacggtatg gttgaccatg ccagtgatga tgagttgttt gccagtgggt
    11821 atttgcgtgg ccacctgaca ttagccatcg cagaactgga aagtggtgat gaccactccg
    11881 cccaggcggt acatacaacc gttagccaga gtctggaaaa agcgattggt gccggtgagt
    11941 tgtcgccgcg tgaccaggcg ctggtgaccg atatgtggga aaacctgttt cagaaggcgt
    12001 cacagcagta agtaatgtgc cggataaggc gttcacgcca tatccggcaa gcaatgccag
    12061 agctgattac cccgccttaa ccgctttacc tttcaacaac ttccgcaccc ataaccgatt
    12121 cgggtttaac gccgccagcg tactggcatc catcggaatt ggttcgttgc tcatctgcgc
    12181 cgccagaatc tcggcacaca acggggcaga gcacaaaccg cgagaaccta aagcagctaa
    12241 cataaaaaga tcgtcataaa ttggcgcgct tactgcctca tctttctgct ccgccagcga
    12301 agcatattcc acgagcgtta cgtcataatc gggcacgttg cctaccattg gcagatgatc
    12361 gcgggtggca caacgcacac cgcagcgcgc ctctttctca ctgacatcaa cctcttttgc
    12421 ccactgcgcc tgcgggaaac aatcaatcaa ccgctggcga ttctgctgct gatcgtcctc
    12481 actgtacgcc gtatcttcgc tgccgcgatg ataactggca ccaatacaat gatgttgatt
    12541 cgccggattt tgtggcgtga gataaccgtc atagcacagc acctgcttca gctccgctag
    12601 ttccggcgtt gtcggaatat ggctaacctg cccggcaacc gaatacactg gcagagacga
    12661 tgtttggctg aaacggctga tttgatgccc gttcgccagt accaccacgc tatgcgttgc
    12721 ttgctgatct cctgcaaaat tcagcaacca acagtcatcc ttacgggata aatcctttaa
    12781 ctggtgttga taacgaatct gtaaaccttg ctgttgcgcc agtttcagca cattacaggt
    12841 cagttctgct ggacacagcc atccaccttg cggatatgta ataccgccac aatttgttac
    12901 tacacctgta acgccttcaa cctcattcgc cccgaccgca acagccagtt ctgtgggtaa
    12961 atccatcgac agcatctgtg cgattttatg ctggcttttc tcatcccagc ctaactgcgt
    13021 gacgccgcac cagtcatgat caaatttgac gggcaataaa tcgtaaagcc gacgagcaaa
    13081 agtaaacgca ttagagaaaa agcggttcaa cgcctcatcg tgtttactta agagcggata
    13141 cagcgccccc tggcgattgc cggaagcacc cagcgcgggg gcttcatccg cgcaataaag
    13201 cgttacttgc cagccgcgcc gtaataacgc cagcgacaac aacgcgctgg caataccacc
    13261 gccgataatc gctacttccc gtttgctgct gcccgtacga ttaaaccacg gcgtggagca
    13321 ggggaggggt aatgtctgtt ccatcacccc acaaagcatt tcccgtttgc gcccaaagcc
    13381 cttacgtttt tgcatcgtga atccggcgtc ctgcaaaccg cggcggacaa aaccggcaga
    13441 cgtaaatgtc gccagcgtgc tgcccggacg cgccagtcgt gccatggcgt taaacagatt
    13501 ttgcgtccac atatccgggt ttttcgctgg cgcaaagccg tccagaaacc aggcgtccac
    13561 tttttgattc agcgagtcgt ccagttggct ggtcagttcg tttatatcgc caaaccataa
    13621 atccagtgtc acgtggcctt catcgagcaa taaacgatgg caaccgggca agggcattgg
    13681 ccactgcgcc tgaagttgtt ctgcccacgg agccagttcc ggccagtgta gatgcgctaa
    13741 ggctaaatcc gcacgggtga ggggaaattt ctcaaaacta atgaaatgta agcgttgtaa
    13801 ttgcgcttgc ggatgtgctt cgcgaaactg atcaaatgcc tgccatagtg tcaggaagtt
    13861 taatccggtg ccgaagccgc tctctgctac cacaaacaga ggatgtggat gctcaggaaa
    13921 gcgtgcctct aattggttac ctccgagaaa aacataacgc gtctcttcca gtccgttgtc
    13981 attggaaaag taaacgtcgt caaaatctcg ggaaacaggt gtaccctcag cattaaattc
    14041 gaggttggca ggttgtatgg cgtagtgttt cacgtaagtt actcgtctga cgggcagtct
    14101 gacgatctta acgatgtgtg tcaggctgcg caaatttctc tattaattgg ctgatcggac
    14161 ttgttcggcg tacaagtgta cgctattgtg cattcgaaac ttactctatg tgcgacttac
    14221 agaggtattg aatgaaacgt gcagtgatta ctggcctggg cattgtttcc agcatcggta
    14281 ataaccagca ggaagtcctg gcatctctgc gtgaaggacg ttcagggatc actttctctc
    14341 aggagctgaa ggattccggc atgcgtagcc acgtctgggg caacgtaaaa ctggatacca
    14401 ctggcctcat tgaccgcaaa gttgtgcgct ttatgagcga cgcatccatt tatgcattcc
    14461 tttctatgga gcaggcaatt gcagatgctg gcctctctcc ggaagcttac cagaataacc
    14521 cgcgcgttgg cctgattgca ggttccggcg gcggttcccc gcgttttcag gtgttcggcg
    14581 ctgacgcaat gcgcggcccg cgcggcctga aagcggttgg cccgtatgtg gtcaccaaag
    14641 cgatggcatc cggcgtttct gcctgtctcg ccacgccgtt taaaattcac ggtgtaaact
    14701 actccatcag ctccgcgtgt gcgacctccg cacactgtat cggtaacgca gtagagcaga
    14761 tccaactggg caaacaggac atcgtgtttg ctggtggcgg cgaagagctg tgctgggaaa
    14821 tggcttgtga attcgacgcg atgggtgcgc tgtctaccaa atacaacgac accccggaaa
    14881 aagcctcccg tacttatgac gctcaccgtg acggtttcgt tatcgctggc ggcggcggta
    14941 tggtggtggt cgaagaactg gaacacgcgt tggcacgtgg tgctcatatc tacgctgaaa
    15001 tcgttggcta cggcgcaacg tctgatggcg cagacatggt tgctccgtct ggcgaaggcg
    15061 cagtgcgttg catgaagatg gcgatgcacg gtgttgatac cccaatcgac tacctgaact
    15121 cccacggtac ttcgactccg gttggcgacg tgaaagagct ggctgctatc cgtgaagtgt
    15181 tcggcgataa gagcccggcg atttctgcaa ccaaagcaat gaccggtcac tctctgggcg
    15241 ctgctggcgt acaggaagct atctactctc tgctgatgct ggaacacggc tttatcgccc
    15301 cgagcatcaa cattgaagag ctggacgagc aggctgcggg tctgaacatc gtgaccgaaa
    15361 cgaccgatcg cgaactgacc accgttatgt ctaacagctt cggcttcggc ggcaccaacg
    15421 ccacgctggt aatgcgcaag ctgaaagatt aattcggagt aggtcggata ggacgcgtca
    15481 gcgtcgcatc cgacgatatg cggcaatgct gcctccggta ctaaagaata agggaaccct
    15541 atggttccct ttttcacatc attgacaatc gccgcctgta ccaggcaaac ttcccgcttt
    15601 gtcgatttcc ttctgaaaag acgtacgcgt taattcctgc caacgcaccg taaccctgaa
    15661 accagagaga tgagacgggg atactcctcg ccttgcgctg cattctggag taatgcatga
    15721 ctgctgtaag ccaaaccgaa acacgatctt ctgccaattt ttcgctcttc cgcatcgctt
    15781 ttgcggtttt tctcacctac atgaccgtag ggttgccgtt gccggttatc ccgctgtttg
    15841 ttcatcatga actgggctat ggcaacacta tggtcggcat tgccgtcggg attcagtttc
    15901 tggctacggt gctgacgcgt ggttacgccg ggcgactggc cgatcaatat ggcgcaaaac
    15961 gttcggcgct tcaggggatg ttagcttgtg gtctggcggg gggcgcgttg ctgctggcgg
    16021 cgattttgcc cgtccccgca ccgttcaaat ttgccctgtt ggtcgtcggg cgtttgattc
    16081 ttggctttgg tgaaagccag ttactgacgg gcgctctgac ctggggatta ggcatcgttg
    16141 ggccaaaaca ttccggcaaa gtgatgtcat ggaacggaat ggcgatttac ggtgccctcg
    16201 ctgttggtgc gccgcttggc ctattgattc atagccatta cggttttgcc gcactggcgc
    16261 tcaccacaat ggcattaccc ttactggcgt gggcctgtaa cggcacagtg cgcaaagtac
    16321 cggccctggc gggggaacgt ccatcgctgt ggagcgttgt cgggcttatc tggaaaccag
    16381 ggttaggtct ggcactacaa ggcgttggtt ttgcggttat cgggactttc gtttcgctct
    16441 actttgccag caaaggatgg gcgatggcgg gctttacgct taccgcgttt ggcggcgcat
    16501 ttgtcgtgat gcgcgtcatg tttggctgga tgccggaccg ttttggcggc gtgaaagtgg
    16561 cgatcgtctc tctgcttgta gaaacggtgg gcttgttgct gctctggcaa gccccaggtg
    16621 catgggtcgc attagcgggc gcggcgttaa ccggagccgg atgttcgctt atctttcctg
    16681 cgctgggcgt ggaggtggtt aaacgcgtac cgtcacacgt tcgcggcacc gcactgggcg
    16741 gttacgccgc gtttcaggat atcgccctcg gcgtctccgg gccgcttgcg ggaatgctgg
    16801 cgaccacgtt tggttactct tcggtatttc ttgccggggc gatctctgcg gtgctgggaa
    16861 ttattgtcac gatactgtca tttcgtcggg gttaacgaac cagccagacc agcatcagga
    16921 taatcgcgac taacagtatc cacagcgcgg gacgcgttgc catattttgc aacgtgtcca
    16981 tcatcggtgg aaaaggatta agcagcggtt gcatatcacg cggatcaatc cctttaaccc
    17041 gccgttgata aagctgatta agaatatcct cggcctgggc agaagaaagc gatgattgcg
    17101 cagaaaggcc atatttttgc tggatatatg ccgataacgc cgccagttca ctggcatcta
    17161 aaggttgttt cagcgccatc tgtagtgatt caagcgtcgg cgtattttgc tggcttagcg
    17221 tctgacgcgc ctgcagccag gtcaccagat ggttaaacag tttcgctgga attaactcgc
    17281 catctttcac cccggaaagt tccagcatcg attgccagat ctgtttgctg ggttctcccg
    17341 ttgccgccgc aagtttggtc accagctgtt tgagcgcatt gtgctccgcc ggtaataacg
    17401 gacggtcggt cgcctcgcgc tgctgcggtt gcggaataac catcttccct tcctgcaaca
    17461 gggttagaat ggtttttaat tgctccggtg agagctgatt cagcggcgtc tgaccaaagt
    17521 tatgacggat ataatccgtg accgcctgac gattattccc cagacgtaaa tactccccta
    17581 attgcgctaa aagctggcgg gcagaatggc ttttttgcgc ggccagcaga cgttgcgcca
    17641 gattgtgctc agcggcaggg aagtgacgcg aaagcagcgg tgaatctccc gacagaccaa
    17701 tatcgtgcct gatacccgcc cacagttctg ctctttgctg ttgcgtcagt gaggtcactt
    17761 tcgtcattaa gctttccagc gaagtacgtt gctgactgga taaaggctga ttgcccgcgc
    17821 cagacggcag gttatctccc tgacctggtg gttgcccagg aggagggccg gaaataggtt
    17881 gtatcattac gtatccttat acctgaaatc ttcgcaagta tgcctggccg cgagattatg
    17941 gcacacttgt ccggttaact ctcgtctcat acaggtaaca caaacgtgaa aatccttgtt
    18001 gatgaaaaat gaaaattatt taacattttg atataaaagg atttatatca attgttgtcc
    18061 acgcaatgac cacattttcg agatatagaa aaagcccgca gtttttacgc tgcggg
//
LOCUS       DAZKJR010000103        17706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKJR010000103 DAZKJR010000000
VERSION     DAZKJR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.104"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..280)
                     /gene="yddM"
                     /locus_tag="ACHOWS_003570"
     CDS             complement(<1..280)
                     /gene="yddM"
                     /locus_tag="ACHOWS_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFQ0049094.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVT"
     gene            complement(280..558)
                     /locus_tag="ACHOWS_003571"
     CDS             complement(280..558)
                     /locus_tag="ACHOWS_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0049095.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            complement(744..1754)
                     /gene="adhP"
                     /locus_tag="ACHOWS_003572"
     CDS             complement(744..1754)
                     /gene="adhP"
                     /locus_tag="ACHOWS_003572"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFQ0049096.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            complement(1888..3585)
                     /gene="maeA"
                     /locus_tag="ACHOWS_003573"
     CDS             complement(1888..3585)
                     /gene="maeA"
                     /locus_tag="ACHOWS_003573"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFQ0049097.1"
                     /translation="MEPKTKRQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            complement(3742..3879)
                     /gene="sra"
                     /locus_tag="ACHOWS_003574"
     CDS             complement(3742..3879)
                     /gene="sra"
                     /locus_tag="ACHOWS_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFQ0049098.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(3981..4196)
                     /gene="bdm"
                     /locus_tag="ACHOWS_003575"
     CDS             complement(3981..4196)
                     /gene="bdm"
                     /locus_tag="ACHOWS_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFQ0049099.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            4541..4972
                     /gene="osmC"
                     /locus_tag="ACHOWS_003576"
     CDS             4541..4972
                     /gene="osmC"
                     /locus_tag="ACHOWS_003576"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFQ0049100.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(5028..5954)
                     /gene="ddpF"
                     /locus_tag="ACHOWS_003577"
     CDS             complement(5028..5954)
                     /gene="ddpF"
                     /locus_tag="ACHOWS_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0049101.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLTTHGCEVRQSLTIREDEREIRCWRAL"
     gene            complement(5947..6933)
                     /gene="ddpD"
                     /locus_tag="ACHOWS_003578"
     CDS             complement(5947..6933)
                     /gene="ddpD"
                     /locus_tag="ACHOWS_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0049102.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPVLTAYGDNNHRCACWYPQQEVISV"
     gene            complement(6930..7826)
                     /gene="ddpC"
                     /locus_tag="ACHOWS_003579"
     CDS             complement(6930..7826)
                     /gene="ddpC"
                     /locus_tag="ACHOWS_003579"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFQ0049103.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(7823..8845)
                     /gene="ddpB"
                     /locus_tag="ACHOWS_003580"
     CDS             complement(7823..8845)
                     /gene="ddpB"
                     /locus_tag="ACHOWS_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0049104.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(8847..10397)
                     /gene="ddpA"
                     /locus_tag="ACHOWS_003581"
     CDS             complement(8847..10397)
                     /gene="ddpA"
                     /locus_tag="ACHOWS_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0049105.1"
                     /translation="MKRSILFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSHPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(10411..10992)
                     /gene="ddpX"
                     /locus_tag="ACHOWS_003582"
     CDS             complement(10411..10992)
                     /gene="ddpX"
                     /locus_tag="ACHOWS_003582"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFQ0049106.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPLAASYPLLADQFTCFISPGTQHAS"
     gene            complement(11250..13649)
                     /gene="dosP"
                     /locus_tag="ACHOWS_003583"
                     /gene_synonym="pdeO"
     CDS             complement(11250..13649)
                     /gene="dosP"
                     /locus_tag="ACHOWS_003583"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFQ0049107.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSMLPLK
                     I"
     gene            complement(13674..13838)
                     /locus_tag="ACHOWS_003584"
                     /pseudo
     CDS             complement(13674..13838)
                     /locus_tag="ACHOWS_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(14215..15534)
                     /gene="digH"
                     /locus_tag="ACHOWS_003585"
     CDS             complement(14215..15534)
                     /gene="digH"
                     /locus_tag="ACHOWS_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFQ0049108.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPTGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMVDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(15665..17200)
                     /gene="gadC"
                     /locus_tag="ACHOWS_003586"
     CDS             complement(15665..17200)
                     /gene="gadC"
                     /locus_tag="ACHOWS_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFQ0049109.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(17356..>17706)
                     /locus_tag="ACHOWS_003587"
     CDS             complement(17356..>17706)
                     /locus_tag="ACHOWS_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFQ0049110.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
BASE COUNT         4359 a         4550 c         4428 g         4369 t
ORIGIN      
        1 gcgtaaccag acggcgcagt cgcgacacat ccaccgtttt ttcagcctct gccaggctcc
       61 aggcgttttg cagattcagc cacatttgcg gcgaactgcc gatcaccacg gaaagtttaa
      121 tcgccatttc cggagtgagc gccgcttttc cggtcagcaa tcggcttgcc gttgagggcg
      181 caatttccat tgctctggca aactcgcgca ggctgacatt aagttcgtcc agtgattcct
      241 gaataatgtc ccccgggcgg ggatgattgg ccattttcat cagtgatagt cctcgtaatc
      301 cagtatgtat gcatcgccat tgacgaattc aaatgtgatt cgccagttgc ccgagacaga
      361 tatcgcccaa attccatcgc gatcgccgct taacgggtgt agtcggtaac cgggcatatc
      421 gatatcagca agacagcttg ctgcatcaat cacagcgagt cggtggcgta atcgtttgac
      481 ttgcgtcggt ataacgccgg aggttttgcc gagaagaaac aagtcacgca atcccttgtg
      541 tctgaaattc attatcatct cttcatcctt gttccttcaa aaggaacact atcactattg
      601 ttccgtgcag cgcaacaaat ttcgctacgt ttttgcgaat tgtgagggga aatatggggg
      661 aggaaaagaa gaagagtgaa attgcggaaa gcgtctcgca aaacaggctg cgacccgaac
      721 atggcagtcg cagcaaaggc ctcttagcgg cggaaatcaa tcaccatacg gccacggatt
      781 ttgccttctt ccatctcggt aaagatggtg ttgatgtccg ctaacggacg cagggcgact
      841 ttcggcacca ctttgccttc ggcggcaaac tggaaggctt cggttagatc ctggcgcgtg
      901 ccgaccagcg aaccgaccac ttcaatacca tccagcacaa ggcgtgggat gtccaggctc
      961 atagactccg gcggcagacc gacagccaca acacgaccgc ctgctcgtac agcatcaacc
     1021 gccgagttaa acgcagcttt agctaccgct gtcaccaccg cggcgtgagc gccaccggtt
     1081 ttttcttgca caattttggc ggcgtcttca gtgcgtgagt taatcgctaa atcagcgccc
     1141 atttcggttg ccagttttaa ctgctcatca ttgacatcaa tggcgatcac tttggcgtta
     1201 aagacattct tcgcgtattg cagggcgagg ttacccagac cgccaagacc gtagatagca
     1261 atccactgac cgggacgaat ttttgacagt ttcaccgctt tataggtggt gacccccgca
     1321 caggtaatgc tgctggccgc cgccgagtcc aggccatctg gcacttttac cgcgtaatcg
     1381 gcgaccacga tgcactcttc cgccatcccg ccatcaacgc tgtatccggc atttttaact
     1441 gaacggcaga gcgtttcgtt accactgtta cagtattcgc aatgaccgca tccttcgtaa
     1501 aaccacgcca cgctggcacg atcgcctggt tttaatgagg tgacacctgg gccaacttct
     1561 gccaccacac cgatgccttc atggcccaga attacgccgg ttttgtcacc aaaatcgcca
     1621 ttcttaacat gaagatcggt atgacataca ccacaacact ccattttcag cagggcttcg
     1681 ccatgtttca gtgagcgcag tgttttatcc gtaacgtcaa catgatgatc cttcgtaaca
     1741 actgcagcct tcatagttcc tccttttcgg atgatgttct gcatagcagg tgaggcaaat
     1801 gagatttatt cgccactacc cagtatggat gagatctgaa aaagggagag gaaaattgcc
     1861 cggcagcttt caccaccggg cgcaggctta gatggaggta cggcggtagt cgcggtattc
     1921 ggcgtgccag aaattatcat caatggcctg ttgcagagct tcggcagacg ttttcaccgc
     1981 cacgccttgc tgctgcgcca ttttgccaac cgcaaaggca attgcgcggg agactttctg
     2041 aatatctttc agttccggta gtaccagacc ttcgccgttc aggaccagcg gcgaatactg
     2101 agcaagcgtt tcacttgccg acatcagcat ctcatcggtg atacgtgacg cgccggaagc
     2161 aataacgccc agcccgatgc ccgggaaaat aaaggcgttg ttacactggg cgatagggta
     2221 gattttatct ttccatacca ctgggttaaa cggactgcca gtggcgacca gcgcgttacc
     2281 ttcagtccag gcgataatgt cctgcggtgt ggcttccacg cgtgaagtcg ggttagacag
     2341 cggcatcacg atcggacgcg gacagtgttt atgcatctca cggataatct cttccgtaaa
     2401 cagcccggtc tgtcctgaga cgccaatcag aatatctggt tttacattac gcaccacatc
     2461 cagcagtgac agcacatcgc tgtcggtatc ccagtcactg aggttttcgc gcttctgcac
     2521 cagtttggtc tggaaaggca gcaggttcgg catcttgtcg gtcagcaggc caaagcgatc
     2581 aaccataaag actttctgcc gcgccgcttc ctcgcttaat ccttcacgct gagtctgggc
     2641 gatgatcatt tcggcaatgc cgcaccccgc agagcctgcg ccgaggaaga caatcttttt
     2701 ctcgctcagt tgaccacctg ccgcgcggct ggctgcgatc agtgtgccga ccgttaccgc
     2761 cgcggtgccc tgaatgtcat cgttaaaaga acaaatttca ttgcgatagc ggttaagtaa
     2821 cggcatcgca tttttttgtg cgaagtcttc aaactgcaac agtacgtccg gccagcgttg
     2881 tttcacagcc tggataaatt catcaacgaa ttcatagtat tcgtcgtcag tgatacgcgg
     2941 attacgccag cccatataca gcggatcgtt aagcagctgt tggttgttcg tcccgacatc
     3001 cagcaccacc ggaagggtat acgccgggct gatgccgcca caggcggtat agagcgacag
     3061 tttaccgatc ggaatgccca tcccgccgat gccctggtca ccaagcccca gaatacgttc
     3121 accgtcagtc accacaatca ctttaatatt atggttcggc acgttttgca gaatatcgtc
     3181 catattgtgg cggttctggt aagagataaa cacgccgcgt gaacggcggt agatctcaga
     3241 aaaacgctca caggctgcgc cgacggttgg ggtataaata acaggcatca tctcatcaag
     3301 atgattgttt accagacggt agaagagggt ttcgttagtg tcctggatgt tacgcaggta
     3361 gatgtgtttg tcgatttcgg ttttgaatcc ctgatactgg atccatgctc gttccgcttg
     3421 ttcttcgatg gtttcgacca cttccggcag taaccccagc aggttgaagt tacggcgttc
     3481 ttccatgctg aaggcgctgc ctttattcaa caacggaaat tccagcagta cagggccagc
     3541 gtaaggaata taaagcgaac gctgtctttt tgtttttggt tccatgtcac tcactctttt
     3601 ttgaatatcc atccctggca cgcttttatc gtctttgctt taccgccagg gcgtcggccc
     3661 caagtataaa gcagataaaa acaaaaacac cattgcgcag gcaatggtgt ttaatcgtca
     3721 ttgaggactg atggttatga attacttttc agcggggcgt tttctgcctg ttgggttatt
     3781 taccacgctg gatttgtcac cttcggtaac tattttgcgc tggttagaaa ttttatggtc
     3841 cagtccaaga atatgacgtg cctgacggtt cgatttcatt ttaactcctc aatcctgtta
     3901 ctagttttaa ggacaacatc gccgtagcga agaaacacgt gctaaacccc taaattaggt
     3961 tgccgatcaa gcatagcacc ttaaagcgta gggtgctgac cactgaccac ataattgatc
     4021 gtttgctggt agatatcact gaggatgtcg ttatcagatg cttccaccca tttggtcagc
     4081 tccatgagaa tgtcatcttc agtgacaaca ccgtgctctg cccgaaggcc ttgctcaatg
     4141 gcattaacca gagcgggttc tgctgttgaa ttttctgcct gataataagt aaacatagtg
     4201 attctccgtg tctgtgtatt tatggtgtct gctacggatc gcagatttat aaagcacatt
     4261 cagcatggca aatatttgcc gcttcgttgt taagattagt cctggttgat gatttttata
     4321 ttttaactcc atgatattga taggtattat tattgtgatg agttgattga tattgatgca
     4381 atatctcttg agttatatat ttccggttat ctattgtgga aatttaattt aagtgcagga
     4441 attatatttt cgccggattt tattcggatt atcctgctta tcctcgtgct gtttctcacg
     4501 tagtctataa tttccttttt aagcccacag gagagcaaca atgacaatcc ataagaaagg
     4561 tcaggcacac tgggaaggcg atatcaaacg cgggaaggga acagtatcta ctgagagcgg
     4621 cgtgctgaac caacagccgt atggatttaa cacgcgtttt gaaggcgaaa aaggaaccaa
     4681 ccctgaagaa ctgattggcg cagcgcatgc cgcatgtttc tcaatggcgc tttcattaat
     4741 gctgggggaa gcgggattca cgccaacatc gattgatacc accgcagatg tgtcgctgga
     4801 taaagtggat gccggttttg cgattactaa aatcgcactg aaaagcgaag ttgcggtgcc
     4861 gggtattgat gcctccacct ttgacggcat tatccagaaa gcaaaagcag gatgcccggt
     4921 ttctcaggta ctgaaagcgg aaattacgct ggattaccag ttgaaatcgt aaagcgttgc
     4981 cggatgacgc gtcaggcgtg tgattgcctg atgcgttgtt agcgtctcta cagcgcccgc
     5041 cagcaccgga tctcacgttc gtcctccctt attgttaatg attgccggac ttcacatccg
     5101 tgggttgtca gagggcagcg ttcgcggaaa aaacagcctt gtggcaacgt gcggtttcct
     5161 ggtagatccg ttttacgtaa tgcccattct tcctccagcg gtttatcaat ggcggggagg
     5221 gaatccagca ataatcgggt gtatggatgt gccggagctg tcagcacctg ttgcgcgtct
     5281 cctagttcca caatctgtcc gagatacatc accgccaccc gatcgctcat atgacgtatc
     5341 accgagacat tgtgtgaaat cagcacataa gtcagctcgc gattttcctg tagcgttacc
     5401 agtaaattga ggatctgcgc ctgcacggag atatccagcg ccgaggttgg ttcatcaagc
     5461 acaatcacgt caggctgcga agagagtgct ctggcaatgg cgatacgttg ccgctgcccg
     5521 ccggaaaacg catgaggcaa gcggtcgaga tactccggac gaatacccac ctgcacagcc
     5581 agctcctctg ccaacgctcg ccgctgttgt tcactactac gcttagcaat ccagagcggt
     5641 tccgtgatga tgcgccacac cggtaagcgc ggattaagcg aagagagcgg gtcctgaaac
     5701 accatctgca taatgcgttg tgatcctgaa cggatgtact gcccgtggct cggttgcagc
     5761 atacccatta aaagctgtgc gagggtgctt ttgccgcagc ctgactcacc gacgatccct
     5821 aaggtttcac cacgacggat ctgtaaatca ataccattga tggcatgaac atgttccgta
     5881 gttttaccaa gccagttttt acgggccggg aaattgatat ggacgtcgcg taacgttaat
     5941 aatgtatcag acactaatga cctcctgctg cggataccaa caggcgcagc ggtggttgtt
     6001 gtcaccatac gctgttagca ccgggacgtt ttcacactgt gcacctgccg catagcaacg
     6061 atcgcgaaaa gcgcagccgt caggcaaatg ggtgaggttt ggtaccgtcc ccggaatagc
     6121 gggtaatggc tggcgcggta ttccatgttc cggtgcgcat tgcagcaaac caatggtata
     6181 cggatgccgg ggatgatgga taacgtctgc cgtcacgccg ctctcaatca cgcttccggc
     6241 atacatcacg taaacgctat cgcacagttg cgacaccacg gccatatcat ggctgatgaa
     6301 cagtaccgca gttccactgg cgcgggcttt atgtttaagc agacgcagta cctgcaactg
     6361 taccgtgacg tccagcgccg tagtcggttc gtcggcaata atcaattgcg gctcgcagga
     6421 gaatgccagc gcaatcatta cccgctggcg cataccacct gaaagctcaa acggatagcg
     6481 cgacataact tccacggcat ccgggatttg catctcttcc agcagggcaa tcgctttagc
     6541 tctggcttcc cgacgactta ttggttgatg atggcggatc acgtccatca tctgaagacc
     6601 tattcgacgt gtcggattaa gggcggtcat cggttcctga aagatcatcg ccactcgtgc
     6661 gccgcgccac tgacgaagct gcttttcccg agcgttaaga acatcttctc ccagcagtga
     6721 aatatgtccc cgatgtacgc aataactgcc cgtcggtagc agacgcataa tcaacattgc
     6781 agtgactgat ttacccgagc cggattctcc caccagaccg acaatttcac cgcggttgat
     6841 tttcaatgac acattgttga gcgcgtgaac gtcgccgtta aaaccgggga aactcaaatg
     6901 cagttgttga atgtccagaa cgggttgggt catgactgct ttcctcctgc tttcgggtca
     6961 agcagatcgc gaataccatc gccaaagaga ttaaacccga cggcggtaag caaaatcgct
     7021 gcccccggaa atgcgcaata ccaccattga tcgagcacat agttgcgacc attcgccacc
     7081 atcgcccccc attcagcact cggttgttga gcaccaaggc caataaatcc caacgtggcg
     7141 gccattaaaa tcgcgctacc gatatccagc gatgcctgca caatcagcgg cggcagggag
     7201 ttacgtaaaa tatgccagtt gatcagatgc cagcgtgacg caccaaaggt tttcgccgcc
     7261 tgaacatagg tatactggcg aacaactaat gtttgccccc gcgccaggcg cacataaaag
     7321 ggaattcgca caatagcaat cgccagcatg gcgttaaaca aactcggccc gagagcggct
     7381 gccagtgcca ttgtcagcac cagcgaagga atcgacagca taatgtccat gatgcgcatg
     7441 ataatcgcgt ctgcgcgtcc gccaagcaca ccggatagac acccgagtag cgaccccatc
     7501 atacctgcaa tggcgaccac cactaatccg gcgagaattg attgctgact gccgaccagt
     7561 acgcggctga acagatcgcg tcccacttca tcggtgccaa accagtgcgc tgcagaaggc
     7621 ggcaaaaggc gggcggttaa atcaatggcg ttgggatcat gcgtcgccag ccacggtgaa
     7681 aaaatcatca tcaatagcat taatacaatg atgactgcgc cggtcacggt aagcgggctg
     7741 cctttcagca tccataccag ttttgcaccg ttaaatcgcg tttgttttgg tgggcgcacg
     7801 gctgacgttt cctcgcttag catcattcac cacctccacg tccgatacgc ggatcaatcc
     7861 acaaatagag caaatccacc accaggttca ccagcacata agcaaacgaa accacgacgg
     7921 caaagcccat cactgccggg aagtcgagcg cctgtattga tgttactacc catgcaccca
     7981 ttccgggcca ggcaaaaacg gtttcggtga gcactgcgcc atacaacaaa tcgcccagcg
     8041 ccagtcccaa tacggtaatc gaggggatca acgcattggg tagcgcataa cataaaacga
     8101 tataccagcc gggcaagccg ctggcccggg cggtacgaat gtaatcttca ctcaattgtt
     8161 ccagcatagc tgagcggatc tggcgagcga caattcccag gtgaacgaac gccagcgtta
     8221 atgccggtaa gatgagatgt tgcagcgcat tgaagaagac ttcaccgttg ccttcaagca
     8281 gcgcatcgag cagataaaag ccggtaacgt gcgttggtgg atccagccag tcatcaagcc
     8341 ttccgccgcc gggaagaatt tgcagatgac cataaaacag cacaatgacg cccagcccaa
     8401 gccagaacgc aggtgtggag attccggtaa tggccattat tcgcaccaga tgatccagcc
     8461 agcgatttcg ccagactgca gagagtatgc ccagcgggat gccaatcaga agtgccagca
     8521 gcagggcaca aaaagccagt tccagcgtcg ccgggaaaaa tatgcgcaac tcttccagca
     8581 ccggacgccc ggtacgaatg gatgttccca ggtcaccctg aaacaggtcg ctgacgtagc
     8641 ggtaaaactg tacgtacagt ggctggtcca gtcccagttg ctggcgaata ttatccacga
     8701 tagcatcgct ggcgcggtca cccgccagta accgcgccgg atcgccgggg atcaggtgcg
     8761 agataataaa cgttatcaca cagacgcccg ccaccacgag caccagcccc cagcagcgtt
     8821 ggcgtaaaat actccagaag gtcacgttat ttactcatgg tattgatatt gaagacctgt
     8881 tccagcatgg gattgaacac aaagcctttc acctctttgt tcatcgccag ttggtagttt
     8941 ttctggaaca ggtacacata agcagcgtca tcaatgacga ttttctgtgc ctgctggtag
     9001 tcccgggtac gctgcgtctg gtcggtggtc gctagcgcat tgcgcagtag cttatcgacc
     9061 tcactgtttt catagaacga gcggttaccc ggcagacctt ttttgtctga ctcaaaccag
     9121 taattcataa acatatacgg gtcggcaaaa tccggactcc agttgccaat cgcgatgtcg
     9181 taatcacctt tacccactct gtcgcgcatg gtggcgttcg ccagcttttc cagcttcaca
     9241 ttgatgccca gtttgttgag actggattgt gtcgccagag cgatcggttc ccagttcgga
     9301 tcattatcag aatagagaaa cgtcaggctg gtgggtttgc tcgtcacttt atcccattcg
     9361 gctttggctt tcgtttcgtc atggttgtat tgcattgccg tcgcatcgta tccccacatg
     9421 ccttccggaa tcgggccgcg catttgtttt ccgttaccac tcagaatgcc gttaaccatg
     9481 ccctgataat cggtagacca ggaaatggcc cgacgcagat ccgcctgatt aagtggcgct
     9541 ttgctgttat tcagatacag ataggtgacg cgcagtgacg gatactctgc cacattgact
     9601 ttattttcct gcttcagggc gttgagttga tccaccggca gcgcatcggc aatgtcaatg
     9661 tcgccacggg agagctgcag gcgacgggag gcactttcac cgataatttt taccgatact
     9721 cgtttaaagt tcggtttatt gccggggtaa tgcggatttg gcaccagaac taattgctga
     9781 cctttttgcc agcttttcag cataaacggt ccggagccag cggtattttg cgcgaggaaa
     9841 ccacgggcat catccgccgc atgctccttt aagaccgccg gattgataat ggatgcaccg
     9901 tcattcgcca gcgtgtagag gaacggtgcg aatgggtggc taagggtaaa cttcaccgta
     9961 tgttcgtcgg gagcatcaat ctttaaatct ttgggaaatg cttctgctgg cccctggccg
    10021 atttttagta accgctcaaa agagagtttt acggcttcgg cagtgacagg tgtgccatcg
    10081 gcaaatttag cattgtcctt cagggtgaac gtccactctt tttgatcgtc agacgctttc
    10141 cagctactag ccagatcgcc ttcaacgtcg gttgagcctt tatcaccgtc cgttttgtac
    10201 tgaaccagtc gctgataaga cgggtaggtc actgtccagt cgttattatc aattgtcacc
    10261 gctgggtcga gggtttgtgg gtcggcagct ttaccgatca ccagcatatc tttcggtacg
    10321 gcggcgtgcg caaccggcat tgttgtggca aggacgatcg cgagcaatgt aggacgaaac
    10381 aaaatcgatc tcttcatgac agtttcctga ttagctggcg tgctgtgtgc caggggatat
    10441 aaaacaggtg aattgatcag cgagtagtgg gtaactcgct gccaggggta attcgaagtg
    10501 ccaccattcg ctcgggatac cgacaaagcc accgccagtc ataatcgcat tcagcaacag
    10561 gcgattgcgc tgagcggcgg gcgggacgga agggtgataa gcatgggaac gttcgtgcat
    10621 ttcatcgaac cctgcaccca tatcgaggat gttcccgtgc tcatcacgaa gcgtcaggtc
    10681 gatcgccgtg ccacggctgt gattagaacc gaccgtcaca tcaacaacat attgcgggtc
    10741 tgggcaggct tgccacaaca tcgcctgtgc ttgttgtggg cgatacgcat cgtaaatcac
    10801 cagttgtaag cctgacagct gggcgatact gatacttttc gccagcgcag taatcgcatc
    10861 cttgtgtaac agacaacgcg cttgctgata aatagcttta cctgtgatgt tatcagcgca
    10921 ggcgtatttc aattcgatct ccagatcagg gaagatcacg gctaaatcaa ccagttcggt
    10981 ggtatccgac ataaacgatt cctgtataag acaaaaattg ctgcgctttc cgcttatgca
    11041 gatctcatgc cacgccggga taagcgccag aatgctggct taaagttata ttttttaact
    11101 tttgatcaac atttgtgcag cgtagtgcag ttttggtgca agagaggaag ttaaggaagg
    11161 aatctcccgg aatcgtagct gaaatcacag tatttaagtg acagtgtcac gttaaatgaa
    11221 aacccgcgtg tgcgggcgag aggaatttgt cagattttca gcggtaacat actgctcatc
    11281 cagcctggaa tttcttcggc gggcaggggg cgggaaaaga agtatccctg aataacgcga
    11341 cagtggatct tgcgtagcat ctcaaattgc tctttggttt cgacgccttc cgccacgacg
    11401 gttaaattaa ggctttgccc aatgctggta atggcttcaa gtaaggcaag gatgcgtttt
    11461 tcggtcagac aacgatcgac aaaacttttg tcaattttga tttccgttac cggaagactg
    11521 actaagcggg ataatccgga aaagcccgtg ccaaaatcat ctaccgataa accgatgccc
    11581 atatcgcgca ggatctgaat gcgtttaaag atttcggtat cgtgttccat catcatgctt
    11641 tccgtgattt ccaccgtcag ctggtggccg tcgatacccc aggcttgcat tgcatcagac
    11701 acctgattag gcagctgatt actgcgaaag tgcagcgccg acaagttaac ggataacgcc
    11761 gggatatgaa tattctggct acgccattct gctaactgac ggcaagcttc cgcgatgacc
    11821 cagcgcccaa tattttcgat ttcaccgatc tcttctgcga gaggaataaa ccgtgaaggg
    11881 ggcacatgac catgcagggg atcgtgccag cgagcaaggg cttcgatgcc gtacagttca
    11941 cccgtttctg cgaagatttg cggctggtaa accagtttca gttggttatt gctaattgct
    12001 tctttcagcg ccgcgcctaa aaccaaacgc tcttttacca tttcgttcat cgccgggctg
    12061 aagaactgcc agccgttacc gccatttttg cgaataaaat ccattgcatt gtgagcagtg
    12121 gagagcaggt aatcgcggtt tttacccacg tcgtagctga tgccaatgct caaggtgagc
    12181 gggaatggtt tatcgtcaat cattatcggc ttgctgacca cattccgtag ctcatcggcg
    12241 atctgcgtaa tgttactgac gtcgttttcc aggctcacga ggacaaactg cgtaccttcg
    12301 atacgacaga gatattgatc cggtttgagt ttttcacgaa agcgattgac cacttccagc
    12361 aatgcctgat cggcccacgc atagccaagg ctatcaatca catcctgaat atggtcaaca
    12421 ccgatgagat acaccaccgg agagacggct ttgtcgacca ggtcatcgag gtaattgtgc
    12481 aggttattgc gatttggcag accggtcatc ggatcaaatt ggatgagttg ttcaatatgc
    12541 tgacggcttt tttcctgttc cagcgccagc gcggccatat gctggctgat atctgccacg
    12601 cgttcgataa aggcgctggt ttctgctcct gacgaggttt taatttgcag gatccccgca
    12661 ggcgcaccat cacgctgacg aatggtcgct gaccagcttt gcgcattttg aacttctgca
    12721 ccgtgcgaag atgacgccca gtgtatcggc atcccgttgc gcagcgcgaa cagcgaaaca
    12781 tgcgattcgt tgagtacaga ttcgatgtta cgacaaatga tttcccccat ttcatgaaat
    12841 ggcgggctgc tgcacatggc ggcgagaata ttgccttcaa gctggcggat ttgcctttct
    12901 tcggtgatat ccgagaatgt cattaccagg ttctgcagat gcgcgagcac gtcataaacc
    12961 gggctgatag aggctttaat ccagattttt tcaccggtgc gcgtcaacag cagaaattcg
    13021 tcctgatcgc gggcggtttt ccatagcaac tgctgtaaac gaatacggtt atcagcaggg
    13081 aattctggaa tgttcaggag tgtatcgggc tgcataccgc tggcttcgtt aatgcagtaa
    13141 ccaaacattt cggtaaatgc gcgattgcac tgcacaatat ggcgttccgg atcgaggaca
    13201 atcaccggtc ggtcgagatg gtcaacggca ataatcaatt gtcgggtctg ttctttttgc
    13261 gccatttcta cgctggcatc ccgtaccagc gccaggtaat aaactttccc ctcggcgctc
    13321 actttcgata gcgcaaaacg ggtccagatt ttactgccgt cttttttctc cagctgcagc
    13381 tcccgactca tcccctcgac gcgagcttta ccaccttcac ggttgtgacg aatgtattga
    13441 ggatgcgcag gacgcaaatc ccgcggaatc agcatatcaa tgttattgcc aatgacttct
    13501 tcacgtttgt acccccagag cttctctgcg gcggggttga aaaacatcac ttcatcattt
    13561 tcgttaatta acaccgcgcc catcatattt tgctcaaggg cggggaaaaa aatgccatcg
    13621 gcggcattat ccgcatcggt tagcttcatg attacctctg catcctggcg catctaaaga
    13681 ctggctttac agagttcaac acgggttcta cctcgtcttt tggcgatata cagagcttca
    13741 tcggctattt gaatgaggcg ctcatagtca ggatgaccat taaacatggc ggcaccgatg
    13801 gaaagtgaga gggcaatatc ctcgccgttt gcggctttaa aataatgtcg acctttatgg
    13861 ttaaaccgca ggccaaaatc aaccaaacac aacgtgcgag atcgactaac tgcaccatat
    13921 tctcctgaaa tatgaagata tactgaaaag aaataagcga cttaggacag tttcaatcta
    13981 cgctactgtt cttgagaaga gtatagccca tcgtaattat ttttcggtga cagcgaatat
    14041 cgtatgtttt ttcatattca tacattttta ttaggggatt tatgactgtt taactaagtg
    14101 tggttaattg cgtagttaat ttgacttaag taagcatgat tactagtggg atatttcaag
    14161 agggtaacaa gccgggggga aagcaccggc ttgttacaaa gtaagaatgg gagtttaact
    14221 gccccaacga ctttgcagat agctgaccgc ttgttgagtc tgtggcttat tcagatagtc
    14281 ctcacggaac aagattgtgc cgctaatttc tggcacggca tcgttaagat cgagctgctt
    14341 tttcagttcc ggtacgccgc cgttaatcat ccagtctggc tctatctttg aaggttcacc
    14401 cactttatag aaggcgatac cgatatacag gcgggtcctg gtcggtttaa cgacatccgc
    14461 ccaccatttt gccaaaacgt cataacgtgc ggcactccgt gagaacggcc agtaaatttg
    14521 gggagcaatg taatccagca atccttgttc cacccatcga cgggtgtcag cgtaggattc
    14581 gtcataggct gccgcgcctc gggtatcgga accgagcgga tcgtgtgatc ggttacgcca
    14641 cacgcctgcc gggctaacgc caaattcgac ttccggctta atgcttttaa tagtgtgcga
    14701 tacctttgca attaactgct gagtattgtt gcgccgccag tctgcttttg acgcaaatgc
    14761 gcctccgtat ttgcggtacg tttcgttatc atttagccgt gaacccggtg actcagtata
    14821 gaaatagtcg tcaaactgca cgccatctac cggatagcgg gaaaccactt ccgcgactat
    14881 tgatgtgatc cagtcctgaa cttcagggat gcccgggtcg aggacaaagc gatcgccgga
    14941 cgttctgatc cagtcgcggt gttgcacata gacgctcgct ggttgttgag acagagtgct
    15001 attcagttcc ctgatagtac cgggcttcgt attaaccgat acgcgatagg ggttaaacca
    15061 ggcgtgtact ttcatcccac gcttgtgggc ttcgtcgagc ataaattgca gcggatcgta
    15121 acctggattt tcaccaatct ttccggtcat aagatcggac cacggcaaaa tttttgatgg
    15181 ccacagggcg gtaccgtccg gcttgacctg gaaaaagacc gtgttgatgc cgagacgttg
    15241 cagatgatcc agtttgtcga ccatcgcctg ttgttgtaca cgggcccggc tggtggggtt
    15301 actaatgtta accgaggaaa ccggtggcca gtcgaggcga gacactgtgg ccagccagat
    15361 gccacgcatc ggttgtgacg attgttgcgt cgtagctggt ggcttcgaac ctgttggtgg
    15421 tgtcaccatt gactctggag gcgtgctttt gcaactacac agtaaaagtg ccagtgcaac
    15481 cagtatcgct ggtcttctaa ttgctaattt ctcgtttcgg gagcagatat ccatatgtgc
    15541 tggtttccgg taaacagatg tgcgctcatt ctcgtactta tcccggttaa gtcaatacga
    15601 caacaagcac gaaaaaggga gcgatgaatt atcgctccct tgtcttataa ccattcagac
    15661 atggttagtg tttcttgtca ttcatcacaa tatagtgtgg tgaacgtgca cgcgggtgca
    15721 ggaagaagtg accttttggt gcgttctgac tgttgattgg ctccagagtg acgccggtat
    15781 tcgctttgct tttacgatca tgaacagcat agagaataaa gggcagggca agtaccacca
    15841 ggaaactcac gaccagtaat tcaacataca tatcggtaga gtcaccctgg atgttatccg
    15901 gcggcaggaa ggaaacaata aacgccataa ttgaagtcag cagaccgaca atagccacga
    15961 ccagtttcac ccctttacca ccagggatat taaatgtgcg ttttaagtca ggatgtttaa
    16021 gaaccaacac aatgtagcca ataaacagca tgaaataagc acacagataa atcaccaccg
    16081 tcagcgccag tgcgatcagg aaggacatgt tgttaccgcc accggtattg gtgaggatga
    16141 tcaacgcgat agaagtaatc accaactgcg aaatgaccag cgttaccggt acgccatttt
    16201 tgttcatttt agcgaatgcc gctggcagca ggtttttctg cgctgtgaca tacatcccgc
    16261 gagaaggacc aacaatccag gaagcgattt ccgccagaac acccagcaac agcagtgcgg
    16321 agatcacgcg aaccgtccac tcaatttccg gtgccacatg tgacatcaga acggtaaagg
    16381 tttgcattac ccctgcggag aggttgattt cattacccgg aatgaccatc gcaatagaca
    16441 aaccgccaac agagcttaag cagattgccg ccaccatcag cagtaacata gccagtggat
    16501 agtcgcgccc gggattgctc atttcattga cgtgggttgc ggaagcttca acgcccatat
    16561 aactcaaaat gaaggcaaca aatacaacca gggtgcccac tttagagaag tcagggaaga
    16621 aggtcttcga atccatttcg atagcaacgg gggcaccgga gtgcagataa atagccgcta
    16681 atgcgatcaa aataaatgca ggtaacagga taccggcgaa gaagccaact ttagcaattc
    16741 gcgccgtgta tttcgtgcca ccaaactgcg ttaatgccag cgcccaaaga atgatgagtg
    16801 ctgcaatagt tttggtaatg gggtcttcat tcagcgctgg ccatttcagg atgtaggaga
    16861 gtgcccctaa cacgaaatag agcatcggaa taaaaccaat ggcgatttgc agatagccaa
    16921 atgagatcgc tgcaaatccc catctcggcc ccagagtatt tgatacccag gcgaagacgc
    16981 caccttcttc ccagccgtcg acggtagcca tttccgcagc acaaagtccc acgggaataa
    17041 accataaaat cccgcctaat agcaggaaga agactaatga aaagcccgat gttgcgaagg
    17101 tagggtattc ataaacagcc attaccatcg atgccgttat ggcaaaaaat ccgagtaatg
    17161 tgagctgctt agctttacct gtctgtactg atgtagccat attatccccc taaaacggta
    17221 ttcctgtcgg aaccgcacct gttttgttct gaagcgtatt cagaacaata ttttccgttg
    17281 ctaatgccag tgaacagact ttggaaattg tcccgaaacg gcttcgtttc gggacaccgt
    17341 taccgttaaa cgttatcagg tgtgtttaaa gctgttctgc tgggcaatac cctgcagttt
    17401 cgggtggtcg ctgagatatt tcagggaggc tttgtagtct tccagcaaca gttcagcaaa
    17461 gtccatttcg aagccgcgac ggcacataat gcgcatcacc acgatgtcgg tggcttcacc
    17521 gcctagagtg aaagccggaa cctgccagcc gcgcagacgc aaacgttcag agaggtcata
    17581 cagggtgtat cccggatctt caccatcttt cagtttgaag caaaccgccg ggatgccttc
    17641 gtccgggcga cccgtacaga tgaactcata cggccccagt ttggcgattt catccgccag
    17701 ataagc
//
LOCUS       DAZKJR010000104        17676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKJR010000104 DAZKJR010000000
VERSION     DAZKJR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.105"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..60
                     /gene="tnaC"
                     /locus_tag="ACHOWS_003588"
     CDS             1..60
                     /gene="tnaC"
                     /locus_tag="ACHOWS_003588"
                     /inference="COORDINATES: protein motif:HMM:TIGR02616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFQ0049111.1"
                     /translation="MCVTSKWFNTDNKIVDHRP"
     gene            281..1696
                     /gene="tnaA"
                     /locus_tag="ACHOWS_003589"
     CDS             281..1696
                     /gene="tnaA"
                     /locus_tag="ACHOWS_003589"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFQ0049112.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1788..3035
                     /gene="tnaB"
                     /locus_tag="ACHOWS_003590"
     CDS             1788..3035
                     /gene="tnaB"
                     /locus_tag="ACHOWS_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFQ0049113.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3167..4342
                     /gene="mdtL"
                     /locus_tag="ACHOWS_003591"
     CDS             3167..4342
                     /gene="mdtL"
                     /locus_tag="ACHOWS_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFQ0049114.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4317..5276
                     /gene="yidZ"
                     /locus_tag="ACHOWS_003592"
     CDS             4317..5276
                     /gene="yidZ"
                     /locus_tag="ACHOWS_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFQ0049115.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5433..6182
                     /gene="yieE"
                     /locus_tag="ACHOWS_003593"
     CDS             5433..6182
                     /gene="yieE"
                     /locus_tag="ACHOWS_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFQ0049116.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6204..6770
                     /gene="yieF"
                     /locus_tag="ACHOWS_003594"
                     /gene_synonym="chrR"
     CDS             6204..6770
                     /gene="yieF"
                     /locus_tag="ACHOWS_003594"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFQ0049117.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6824..8161)
                     /gene="adeP"
                     /locus_tag="ACHOWS_003595"
     CDS             complement(6824..8161)
                     /gene="adeP"
                     /locus_tag="ACHOWS_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFQ0049118.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8328..8993
                     /gene="yieH"
                     /locus_tag="ACHOWS_003596"
     CDS             8328..8993
                     /gene="yieH"
                     /locus_tag="ACHOWS_003596"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFQ0049119.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9060..9533
                     /gene="cbrB"
                     /locus_tag="ACHOWS_003597"
     CDS             9060..9533
                     /gene="cbrB"
                     /locus_tag="ACHOWS_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFQ0049120.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITHLPGLDISCPETDSLS"
     gene            complement(9615..10337)
                     /gene="yieK"
                     /locus_tag="ACHOWS_003598"
     CDS             complement(9615..10337)
                     /gene="yieK"
                     /locus_tag="ACHOWS_003598"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0049121.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLHNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(10352..11521)
                     /gene="yieL"
                     /locus_tag="ACHOWS_003599"
     CDS             complement(10352..11521)
                     /gene="yieL"
                     /locus_tag="ACHOWS_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFQ0049122.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPALKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11548..13164)
                     /gene="bglH"
                     /locus_tag="ACHOWS_003600"
     CDS             complement(11548..13164)
                     /gene="bglH"
                     /locus_tag="ACHOWS_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFQ0049123.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13250..14644)
                     /gene="bglB"
                     /locus_tag="ACHOWS_003601"
     CDS             complement(13250..14644)
                     /gene="bglB"
                     /locus_tag="ACHOWS_003601"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFQ0049124.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14663..16537)
                     /gene="bglF"
                     /locus_tag="ACHOWS_003602"
     CDS             complement(14663..16537)
                     /gene="bglF"
                     /locus_tag="ACHOWS_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFQ0049125.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     MPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16671..17507)
                     /gene="bglG"
                     /locus_tag="ACHOWS_003603"
     CDS             complement(16671..17507)
                     /gene="bglG"
                     /locus_tag="ACHOWS_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFQ0049126.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIVKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4502 a         4439 c         4118 g         4617 t
ORIGIN      
        1 atatgtgtga cctcaaaatg gtttaatact gacaacaaga ttgtcgatca ccgcccttga
       61 tttgcccttc tgtagccatc accagagcca aaccgattag attcaatgtg atctatttgt
      121 ttgctatatc ttaattttgc cttttacaaa ggccatcttt cgtttattta cttgttttag
      181 taaatgatgg tgcttgcata tatatctggc gaattaatgg atattgcaaa tgtaatattc
      241 acagggatca ctgtaattaa aataaatgaa ggattatgta atggaaaact ttaaacatct
      301 ccctgaaccg ttccgcattc gtgttattga gccagtaaaa cgtaccactc gcgcttatcg
      361 tgaagaggca attattaaat ccggtatgaa cccgttcctg ctggatagcg aagatgtgtt
      421 tatcgattta ctgaccgaca gcggcaccgg ggcggtgacg cagagtatgc aggccgcgat
      481 gatgcgcggc gacgaagcct acagcggcag ccgcagctac tatgcgttag ccgagtcagt
      541 aaaaaatatc tttggttatc aatatactat tccgactcac cagggccgtg gtgcagaaca
      601 aatctatatt ccggtactga ttaaaaaacg cgagcaggaa aaaggcctgg atcgcagcaa
      661 aatggtggcg ttctctaact atttctttga taccacgcag ggccatagcc agattaacgg
      721 ctgtaccgtg cgtaacgtct atatcaaaga agccttcgat actggcgtgc gttacgactt
      781 taaaggtaac tttgaccttg agggattaga acgcggcatt gaagaagttg gcccgaataa
      841 cgtgccatat atcgttgcga ccatcaccag taactctgca ggtggtcagc cggtttcact
      901 ggcaaactta aaagcgatgt acagcatcgc gaagaaatac gatattccgg tggtcatgga
      961 ctccgcacgc tttgctgaaa atgcctattt catcaagcag cgtgaagcag aatacaagga
     1021 ctggaccatc gagcagatca cccgcgaaac ctacaaatat gccgatatgc tggcgatgtc
     1081 cgccaagaaa gatgcgatgg tgccgatggg cggcctgctg tgcatgaaag acgacagctt
     1141 ctttgatgtg tacaccgaat gcagaaccct ttgcgtggtg caggaaggct tcccgacata
     1201 tggcggcctg gaaggcggcg ctatggagcg tctggcggta ggtctgtatg acggcatgaa
     1261 tctggactgg ctggcttatc gtatcgcgca ggtgcagtat ctggtcgatg gtctggaaga
     1321 gattggcgtt gtctgtcagc aggcgggcgg tcacgcggca ttcgttgatg caggtaaact
     1381 gttgccgcat atcccggcag atcagttccc ggcacaggcg ctggcctgcg agctgtataa
     1441 agtcgccggt atccgtgcgg tagaaattgg ctctttcctg ttaggccgcg atccgaaaac
     1501 cggtaaacaa ctgccatgcc cggctgaact gctgcgttta accattccgc gcgcaacata
     1561 tactcaaaca catatggact tcattattga agcctttaaa catgtgaaag agaacgcggc
     1621 gaatattaaa gggttaacct ttacctacga accgaaagta ttgcgtcact tcaccgcaaa
     1681 actgaaagaa gtttaattaa aacactcaga gtggctataa ggatgttagc cactctatta
     1741 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaat
     1801 cagaaaaaaa acactctgca ttttggggtg ttatggttat agccggtaca gtaattggcg
     1861 gtgggatgtt tgctttacct gttgaccttg ccggagcctg gtttttttgg ggcgctttta
     1921 ttcttattat cgcctggttt tcaatgttac attcagggct attattatta gaagcaaact
     1981 taaattatcc cgtcggatcc agttttaaca ccatcaccaa agatttaatc ggtaacacct
     2041 ggaacattat cagcggtatt accgttgcct tcgttctcta catcctcact tatgcctata
     2101 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggt tatcatgcta
     2161 atccacgtat tgtcgggatc tgcactgcca ttttcgttgc cagcgtattg tggataagtt
     2221 cgttagccgc cagtcgtatt acctcattgt tccttgggct gaagattatc tcctttgtaa
     2281 ttgtttttgg ttctttcttc ttccaggtcg attactccat tctgcgcgat gccaccagca
     2341 ccacggcggg aacatcttat ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2401 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2461 agctaatcaa aagcgtggtc ttcggttcgc tgctggcgct ggtgatttat ctcttctggc
     2521 tctattgcac gatggggaat atcccgcgag aaagctttaa ggcgataatc tcctcaggcg
     2581 gcaacgttga ttcgctggtg aaatcgttcc tcggcaccaa acagcatggc attatcgagt
     2641 tttgcctgct ggtgttctcc aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2701 gattgttcga ttatctggcg gatctgttta agattgataa ctcccacgcc gggcgtttca
     2761 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2821 tcatttacgg gatcggcggt gccggactgt gtgccaccat ctgggcggtc attatccccg
     2881 cggtgctggc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     2941 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtaatttta tgctggttcg
     3001 gtaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3061 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct gatcctcctt
     3121 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3181 gatttgtagt tttgccctgg ttttacttta tcccgcaggg attgatatgt acctcgttgg
     3241 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3301 cgtttatctg gcggggatgg cggctgcgat gttatttgcc ggtaaagtgg ccgatcgttc
     3361 agggagaaag cctgtcgcca tacccggcgc ggcgctattt attatcgcct cggtgttctg
     3421 ttcactggct gaaaccagcg cgttatttct tgcagggcga tttctacagg gactgggcgc
     3481 gggctgttgt tacgtagtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3541 ggctaaagtg ctgtcgttac tcaacggcat tacctgcatc attccggtgt tagcgccggt
     3601 gctcggacac ctgattatgc ttaaattccc ttggcagagt ctgttctgga cgatggcaac
     3661 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gccctgcggc
     3721 ccccacaact tcggacaaac cacgagaaaa tagcgagtca ctgcttaatc ggtttttcct
     3781 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcaccttcg tcaacacgtc
     3841 gccagtattg ctgatggaaa tcatgggttt tgagcgcgga gaatacgcca ccattatggc
     3901 gttgaccgct ggcgtcagca tgaccgtttc attctccacg ccgtttgcgc tgggaatttt
     3961 taagccacgt acgttgatga tcacctcaca ggtgttattc ctggcggcgg ggatcaccct
     4021 tgccgtttca ccttcccatg cggtttcgct gtttggtatc acgctgattt gcgccggttt
     4081 ctcggtaggt tttggtgtgg cgatgagtca ggcgttaggg ccgttttcat tacgcgcggg
     4141 cgtagccagc tcgaccttag gtattgcgca agtttgcggt tcgtcactgt ggatttggct
     4201 ggcggcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4261 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgcccggttg ccgctcatga
     4321 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4381 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcggcg gtgagtaagt
     4441 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4501 tgtcgcccac accgctaatg gtcagcatgg agcaaaatct ggcggagtgg atgcaaatga
     4561 gcaacctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagctgg
     4621 cagcagaatc accgctaatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4681 gttacccgca ggcgaccatc aaattacgta actgggatta cgattcctta gatgccatta
     4741 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag tcatccccgt tcgcgggagc
     4801 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtgccctgcg
     4861 tctggttacg caaagatcat ccggcactgc atgaagcatg gaatctggac acctttttac
     4921 gttatccgca tatcagcatt tgctgggaac agagcgatac ctgggcgctg gacaatgtgt
     4981 tacaggagct ggggcgcgaa cgcacgattg ctatgagcct gccggaattc gagcagtcac
     5041 tgtttatggc agcacagccc gacaatctgc tactggcgac cgcaccgcgc tactgtcagt
     5101 actacaatca actccaccaa ctgccgttgg ttgctcttcc tctcccgttt gacgaaagcc
     5161 agcaaaaaaa gctggaagtc ccttttaccc tgctgtggca taaacggaac agccataatc
     5221 cgaagatcgt ctggttacgg gaaaccatta aaaatcttta cgcgtcgatg gcataaccga
     5281 atcgtatgaa acgcgaccca atttcacaat aaaatgtaaa aaagttgtaa taagcttgtc
     5341 tgaatcgaac ttttagccgc tttagtctgt ccatcattcc agtaaatgat tactcttgta
     5401 ttcataatgg accattaagc atggagcgaa aaatggcgac tcactttgcc cgagggattt
     5461 taacggaagg acatctgatt tctgttcgtc tcccctccca gtgtcatcaa gaagcccgaa
     5521 acattccccc tcatcgtcaa agccgttttc tggcgtccag aggtttactc gcagaactga
     5581 tgttcatgct gtatggcatt ggcgaattgc cggaaatcgt caccctgccg aaaggaaaac
     5641 cggttttcag cgataaaaat ttgccttcgt tttctatttc ctatgccggg aatatggtcg
     5701 gcgtggcgtt aacaaccgaa ggtgaatgtg gcctcgatat ggaactacag cgtgcgacgc
     5761 gcgggtttca tagcccacac gcgcccgata accacacctt ttccagcaat gaatcgctat
     5821 ggatcagtaa acaaaacgat cctaacgaag cgcgggcgca gctcatcacg ctgcgccgaa
     5881 gcgtgctaaa actaaccggt gatgttttga atgacgatcc gcgcgatctg caactactgc
     5941 ccattgccgg acgcctgaaa tgtgctcatg taaatcatgt agaagcgtta tgcgacgcgg
     6001 aggacgtgct ggtgtggtcc gtggcggtca cgcccacgat tgaaaagctc agtgtctggg
     6061 agttagatgg caaacatggc tggaaaagcc tgcccgatat tcacagccgc gccaacaatc
     6121 ctaccagccg gatgatgcgt tttgcccagc tctctaccgt gaaggctttt tcgccaaatt
     6181 gatagacaac cacaggagtc atcatgtctg aaaaattgca ggtggttacg ttactgggga
     6241 gcctgcgcaa aggctcattt aatggcatgg ttgcacgtac cctgccgaaa attgctccgg
     6301 cgagcatgga aatcaatgcg ttaccttcca ttgccgacat tcccctgtat gacgctgacg
     6361 tacagcagga agacggtttt ccagcaacag ttgaagctct ggcggaacag atccgtcagg
     6421 ctgacggtgt ggtgatcgtc acgccggaat ataactactc ggtaccgggt gggctgaaaa
     6481 atgccatcga ctggctttcc cgcctgccgg atcaaccgct ggctggtaaa ccggtattga
     6541 ttcagaccag ctcaatgggc gtgattggcg gcgcgcgctg tcagtatcac ctgcgccaga
     6601 ttctggtttt cctcgatgca atggtgatga acaagccgga atttatgggc ggcgtgattc
     6661 agaacaaagt tgatccgcaa accggggaag tgattgatca gggtacgctg gaccacctga
     6721 ccgggcaatt gaccgcattt ggtgagttta ttcagcgagt taagatctaa ataaaaaacc
     6781 cgccagcaat catgcatggc gggtttttaa cgcgctatcg attttagtga gcgtcgataa
     6841 agacaatctt caggataaac agcagcgcaa cgatgattac gcacgggcta aggtcacgca
     6901 gacgtccggt accaatcttc atcacgcagt aggagataaa gcccagcgcg ataccttcag
     6961 taatcgagaa gctgaacggc atcatcacag cggtaataaa cgccggaaca gattcagtaa
     7021 gatcctgcca gttcacgcga gcaagacttg aggtcatcaa cacgccaacg taaatcagcg
     7081 caccagctgc agcgtagcct ggcaccatcc ccgccagcgg cgacaggaag ataaccagca
     7141 ggaacagcag accaacaacc actgccgtca gaccggtacg accgccaacg gaaacgccgg
     7201 aagaagattc aatatacgcc gtaacagaag aagtaccgat aaacgaaccg gtcaccgaag
     7261 agatactgtc gacatacagc gcctgcttca tgcgcgggaa tttacctttc tcatcagcca
     7321 gacctgcttt atcggtcacg ccaatcagcg taccggagga gtcaaacaag ttgaccagca
     7381 tgaaagagaa aatcacccct gccagcccaa ggttaaacga cccggctaaa tctacatgac
     7441 ccacgacact cattacgctc ggcggcgcag aaacgatgcc attatagtgg acatcaccca
     7501 gcatccagcc cagcagcgtt gtcaccacga tggaaaccag caccgccgcg tgaatattgc
     7561 gcgaggccag aatggcgatg atgaagaagc cgaggatccc cagcagtacg ctgtgagaag
     7621 tcagattacc gatgctcacc agcgtttccg ggttagcgac aatcacacct gcattcttca
     7681 gtcccatcat gccaataaac aggccgatac cgctggtaat gcccacacgc agactcaccg
     7741 gaatgttggc tatcatccag tagcgaacgc ggaaaatcgt cagtaacagc agaccaatcg
     7801 cgccccagaa gattgcgccc atcccgacct gccacggcaa gcccatcgcc tgcacaacca
     7861 caaaagcgaa gaacgcattc aggcccatag cgggtgccag tgcaactggc aggttagcaa
     7921 acagtcccat cataatactg ccgaatgcag caatcagaca ggtagtaacg aagacggcgc
     7981 tggtatccat gccagcaacg ccaagaattt gcgggttaac aaaaacgatg taaaccatcg
     8041 tcaggaaggt ggtaaaaccg gcgatcactt cggtccgtgc cgtcgtgcca tgttcgcgca
     8101 gtttaaacac gcgttccagc atcccctggc cagaagcctg ggttgtgtgt tgttgactca
     8161 ttatctattt ccgaacaagg agggaaaatc cgtcgctatc gtataccaaa atgagacaat
     8221 atggacggct gaagataact ttttttcatt gtatttgctt atacggcaac gattgcgtga
     8281 tgcgaattgg catgacgaaa acgttaaact aacgataaag gaaagacatg tcccagatag
     8341 aagcggtatt tttcgactgc gacggtacgc tggtcgatag cgaggtcatt tgctctcgcg
     8401 cctatgtcac gatgttcagg gaatttggca ttcacgttga cctggaagag atttttaccc
     8461 gttttaaagg cgtaaaactc tacgaaatca tcgacatcat cagtaaagaa cagggcgtga
     8521 cgatggtcca ggccgatgcc gagcacgttt accgcgcaga agtagcacgt ctgtttgata
     8581 ccgaacttga ggacatcgca ggagcgaacg aattgctggc gagcattaac gtgccgatgt
     8641 gcgtcgtctc taacggtccg gtcagcaaaa tgcagcattc gcttggtaag ctcaacatgc
     8701 tgcactactt cccggaaaaa ctgttcagcg gctacgatat ccagcgttgg aagcccgacc
     8761 cggcgttaat gttccatgcg gcaaaagcga tgaatgtgaa cgttgaaaac tgcattctgg
     8821 tcgatgactc cagcgccggt gcgcaatcag gaatcgatgc tggtatggaa gtgttttact
     8881 tctgcgctga cccgcacaat aagccgatcg ttcatccgaa agtcaccacc tttacccatc
     8941 tttcgcaatt accggaactg tggaaggcgc gtggttggga tattacggca tagttattca
     9001 cactcccttc acttactcca cttaaattgg cacgcaaagg taagtaaagg gagtttgata
     9061 tgtctgtttc acgtcgggta attcatcacg gactgtattt tgcggtttta gggccgttaa
     9121 ttggtgttct gtttcttgtc ctctacatat tcttcgcaaa agaaccgctg attcttttgg
     9181 taataataca ggtacttcca ttgtttctct taatgtcgat aactacggga gctattcctg
     9241 caatgttaac tggcgtaatg gtcgcctgtc tgccggaaaa gatcgggtca cagaaacgtt
     9301 atcgttgtct ggttggcggc ataggtggag tcgttatcac cgagatctat tgcgctgtta
     9361 tcgtacatat taaggacatg gcttcctcag cgttgtttga aaacattctt tctggtgaaa
     9421 atctcgttgt ccgcatcatc ccagcattgc tggcaggcgt agtgatgagc agaatcatta
     9481 cacatctacc cggattggat atttcatgtc ctgaaacaga ctctttaagc taagcggaat
     9541 gctttatcta tttgtcatta aatgttggag aacaggccgg agcgtaattc acacatccgg
     9601 ccttattttt aagcttaacc gagcgctaat tctgcggcag cggctttatc agcgatgact
     9661 atcagcgaag ggtgcaactg caatacggaa gcaggaacat cttcggtaac cggaccttgc
     9721 agaacatttt tcagtgcctg cgctttaccc gcgccactga caattatcag caagtttttc
     9781 gccgccatga tgcttttcgg ccccatcgtc acgtagctgt caggcactaa tgaaaagtcg
     9841 ccacccaact cgccatgcgc cacaatatca accatctctc cctggatcgg gaactccacg
     9901 gtttgctcat ggaaatgggt cgtatttggc agattgccac aaaaatgacc atctacacct
     9961 aatcccaaca ccaccaaatc caggccgcct tcacgcgcca gtttctgatc atgctcgcgg
    10021 tagttatcaa tagtgagctt ctggatattc tcttctttga tccctgcagg ggtgaaaaac
    10081 agattatgca gattggtaat cgttacgcct tccccttctt taccgcgaaa tggaatttca
    10141 tcgaaattgt agaaatagca gttatcgtac cagggcttac ctttaaccag agtgatgaga
    10201 tattcgtaca tgcctttggg cgtgctcccg gcggtaattg ccaggttaac gcgacgcatc
    10261 tttgacatat aaccaagcag atgatgtgcc gcgacacggc tcatttcctg gtaatcttcg
    10321 gtaatgatca atttcatctt aatgccttat attatttgaa taatttctgt acaaaggctg
    10381 cataggctgg tcgccagaca tccatttcat ggttcagacc gggatattcc tggtaatcaa
    10441 acttaatttt tttctgctca agctcagttt tcagcccggc gatatccttg ccggttacga
    10501 catctttatc gcccacgacc acagtaaaat tacgtagttg ctggttaata gctgccggct
    10561 cgttcagccg ggcagcgacc ccttcatctg gtacggtcgt cgtggtaaca ccactgaatg
    10621 tggccagcca gccaaagctt tccagatgat tcattcccga taccagtgcc tgatacccac
    10681 cttgtgaaag ccctgccagt gcgcgaccat cggcatcttt acggacatta aaacgcttgc
    10741 taatcagcgg gataatatcg ttcatcaatt cgcgatctgc cgctttagca tttaacggat
    10801 aaaagacttt acgtctctcc tgaggaacga aatcttcggg aataatgccc ttcgcatcgg
    10861 tttcggtatc cgggatcacc accagcatcg gtttaatttt cccttcagcc agcaggttat
    10921 ccatgatttg cgggatacgc ccctgatcaa tagcggaacg tccggtatca ccaaagccgt
    10981 gatagaaata gagcactggc aaaggctcgc ctatgccggt gtatcctggc ggggtccaga
    11041 catacatctg acgttcggat tgcaatgcgt tggagtggta agttatggcg atcaaatcac
    11101 catgcacaac agaacgtgta tccagataac tgcccggtac cagaatcata ctggagttaa
    11161 cctggcgctg aggcttagtc attgctgtac ctgtatcaat gctgcgtaca ccatcaacat
    11221 tgaaaaaata ctcgtacagg ttgcctttca gagcaggcgt gcgccacgac cagactcccg
    11281 cttcgtcttt ggtcatcggg tgaatattgt ccggaaccgg aacacccacc actacagaga
    11341 cattttttgc cccaggggca aagtagcgaa aggtcacgct gttatcggta ttgacctgag
    11401 tcacatactg tttcaccgga atagtgggtg ccgggctggc tggcatatcc gctgctatgg
    11461 cccattgagc agaaataccg ctggcaatag ccagcgttaa agcagcaatt tttatattca
    11521 tcgtaatatt ccttttatta agttccatta ccaccagatt tcagcctggg caccgacagc
    11581 aaactggtcg tctttattgt cttcaaaggt gaagccatcc agttcgtttt ccagagcttt
    11641 gatatatgta gcgtagaaac gaatttccgg gcgcgaggtc aacatactgg tattgacttt
    11701 aaaggtatgg aagagcgtgg ttttataacc agactcatta tatttattgc tattcgcatc
    11761 tttgttttgc tgggtgaaat agcccagttc aacacctgtc tggttatatt ggtcccaaat
    11821 ataggccggg cgaacaaccg cacgaataga ttcgaaatca gaatgtgcgc ctgtttcgta
    11881 gctataaata tcgttaccga aggagtaaac aatcgcgtta gccacaatga aatgatcgcc
    11941 gatataagct tcgccctgcg aagtcagacg aactgcagtt ccgcctgtgt gatcaccata
    12001 ataacgacca ttaaaggtag taaatggact tgcaccagca taacgaccaa agttactggc
    12061 gatagagtta ttcgcaacca ggaaggagaa ttcgttgaag ccacccttat caaatttctg
    12121 cgttaaagat gtaccaaaca tccaggtatc tttccagtca taatacccgt tattatcctg
    12181 attatctttt tccgatgcgc tttcatttgc tgagacataa cgaccgctca ccattaaggt
    12241 ggctttatcc cataacggga tatctttata gcgtaaatca atggtattgg tattaatctg
    12301 ctgtttgttt tgcaggctgc gatcgtaatc atcgacatct tcacgaacca gcgcgatatc
    12361 aattttaccc ggaccgactt tccagttttc cagacctaca cctgcggcag catcggtacg
    12421 ctgcgttttc cagtcaagca tctggatttc aattttcggc gcaccgtgtt tacccaccca
    12481 gaaatcagcc tctggcgcaa agggcaggaa acctttggtg gtaacgtaca tatcagagaa
    12541 ctgcataaag ttctcaccac cggcgttatc gccaaaccag ccagtagagt actgctgacc
    12601 aacgttacca tccatcatca cgatggcatc aacccgtttg ccgttttcgt tgtagacacg
    12661 ttgttttaac tgcaaatcaa accagccgga gtattcgtta ccaaagcggc ccagagaacc
    12721 aattgcccat gatttaggtg aaccatgaga ggctgttccc caaccagaac ggtagtagcc
    12781 gttgtaacta aagccgattt catctttcac aaatttactg aaatctttca gagtcatgga
    12841 agaataaatc gagcctgtct cactggcatt tttctcttca tttttaacca gtacagcatc
    12901 aggctttgcg gcacgactgg tcgggaatgg attggcggca tacccttgat cattcgtact
    12961 tacgctacga ttcaccgtcg ctggcgtata ctttttcttc tcttcatctt tatatttttt
    13021 cagttcgctt tgcgtttctc ttaacgcctt ttctaatagc tcaaggcgtt gttccactgt
    13081 taatgattca gccaatgatt gtgcggaaaa ggctaacggt gccattaata agatggcaga
    13141 ggtaataaga tttcgtctaa acataatatc cctttatggt gcaaagaaag aattaacgca
    13201 tcgcatccag actgttctga atgcgacgat aattaaggtg ctttaatggt tattttttta
    13261 atgacagccc ccgagttttg atcacttctg cataccatcc gaagcttttc ttgcgcgttc
    13321 ttgtgaggct tccttcacca ttatcatcac gatccacata aataaagccg tagcgcttag
    13381 acatttgtga atgagatgca ctgactaaat caattggccc ccaactggtg taccccataa
    13441 tatccacacc atcggcaatc gcttcattta cctgtaccag gtgatcgttt aaataggcaa
    13501 ttcgataatc gtcctgtatc gaaccatcca cttcaacgct gtcttttgcg cctaatccgt
    13561 tctcgacaat aaataacggt ttttgataac gatcccaaag cgtatttaac agaatccgta
    13621 atccaaccgg atcaatttgc cacccccact ctgaactttt cagatgcgga tttgggatca
    13681 tattcagtat gttgccctgc gcatttttat taatgctttc gtcgtgggaa acacaaccgg
    13741 tcatgtaata actaaaagag atgaaatcta cggtatgttt taaatcttct gcgtcacttt
    13801 cagtcatctc aatggtgata ttgtggtcgc ggaagaaacg ctgcatatag ccgggatact
    13861 gaccacgcgc ctgaacatca ccaaagaaca tccagcgccg gttctcttcc atggcctgca
    13921 acatatcctg tggctggcag gtgagggggt aaaccagccc accgagaagc atattgccaa
    13981 ttttcgcttc ggggatcagg ctatgacagg ctttaactgc ccgcgcactg gcaaccagtt
    14041 gatgatggat cgcctgataa acttccgcct cgccactctc ttctgccagc cccactcccg
    14101 tgaatggcgc gtgtaatgac atgttgattt cattaaatgt cagccataac acaactttat
    14161 gttggtagcg ggtgaagacc gtgcgggcgt aatgctcgaa gtgatcgatg accgcccgat
    14221 tagcccaacc gccgtagttt ttcaccagcc catatggcat ttcgtaatgg gataacgtta
    14281 ccagcggctt gatccccgcc tgcgccattt catcaaacag ccgatcgtaa aacgctaacc
    14341 ccgcttcatt cggttcggct tcgtcgccct gagggaaaat tcgcgcccag gcaatggaaa
    14401 tacgcagaca ggtgaagccc atctcggcaa ataacgcgat atcttccggg taacggtgat
    14461 aaaaatcgat ggcgacatct ttgatattct ctttccccag gatgcgcggt tccatttttc
    14521 ccattacgcc atgaggctgt aaatctgagg tcgtgatccc tttgccatct tcctgccagg
    14581 cgccttccac ctgattggca gctgttgcac caccccaaag aaatgtttct ggaaatgctt
    14641 tcataattaa ctccttttat cgttagcgaa tgatggataa cagcggctca ccagcgttta
    14701 tctgcgccgt gccgtggggt aatacgtccg taaaatcatc gctattactg attaataccg
    14761 gcgtcgtcag atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa atcagccgat
    14821 cgcctgtatt gaccttgtca cccacgttga cgtgagcgga aaagaatttg ccgtccagtt
    14881 ttacggtatc gataccgaca tgaatcagga tctccacacc atcatctgac tcaatgccaa
    14941 tggcgtgtaa tgtggcgaac aacgaagcaa ttcgacctgc caccggagaa cgcacttcac
    15001 caaccgaggg cataatggca atacctttac ccaacaggcc gctggcaaac gtggtatcag
    15061 cgacgtgaat gagcggcaca atctctcccg tcatcggtga acagataccg ccctgctcag
    15121 gtgtaataac ctctggtgtt ttctcttgct gggcaacttg cgctgactga ctcttagcgg
    15181 tgatgaaatg aaacatcacc gtaccgacaa atgcgcaacc gatggcaatg acaccgccaa
    15241 taacgctggc ccatacggtg aaatcaatcc ccgttgacgg gatggtttgc atgaaggtga
    15301 aaatacctgg caaaccaaag gagtaaactt tcgtttgcgc gtagccaatg atggtggccc
    15361 ccaatgcccc acttatgcag gcgataacga aggggtactt acgcggcaga ttgacgccat
    15421 ataccgctgg ttcggtgata ccaaacaaac ctgtcaacgc cgctgatccc gccaccactt
    15481 ttttctgtgc atcgcgttcg cagaggaaga cgccgagcgc cgccccgact tgcgccataa
    15541 tggcgggcat taacagcggg atcatggtgt cgtagcccag cacggtgaag ttattgataa
    15601 ttaaaggtat caaaccccaa tgcagaccga acatcacaaa gatttgccag aagccgccca
    15661 ttaccgcgcc tgcaaatgca ggaaccgcct gataaagcca gagataaccg gcagcaatca
    15721 gttcacttat ccaggttgat agcggcccca ccagcagaaa tgtgatgggc gtgatgacca
    15781 tcagacatag caagggtgtg aagaaatttt tgattgccga aggtaaccac gcattaagtc
    15841 ggcgttccag aatgctgcac aaccaggcag aaaaaataat gggaataacc gatgacgagt
    15901 aattcaacaa tgtgaccgga atacccagga aatccaggcc cagcgcatcc actttttgcc
    15961 cgttctcgaa ggcagtcaga attaatggat gcactaacgc tccgccaatc accatggcag
    16021 taaatggatt gccgccgaag cgtttccctg cggtgtagcc caggattatc gggaagaacc
    16081 aaaacaaggc atcactggcg ctaaataaaa ttaaataagt accactttgt tcggtcgtcc
    16141 actgaaaagt gagtgccaga gccaacatac ctttcaggat cccggttgcc gccatcaaac
    16201 cgatcagagg cgtaaaaata cctgaaataa cataaacaaa gcggtttaac agattacctt
    16261 tatcatcatt ttccggtgcc tgttgcgctt tttcgccaag gcctgccacg ctgttaaccg
    16321 ccaggaagac atcggccaca tggttaccta tgacaacctg aaactggcca ccgctttcca
    16381 ccaccataat aataccgggg gtctttttca gtacctcagc ttgcgctttg ctttcatcct
    16441 ttaacttaaa acgtaatcgc gttgcacaat gcatcagact cacaatgtta tctgcgcccc
    16501 cgactcctgc gactattgtt ctggctaact ccgtcataac ttgccctcta ccgctttgcg
    16561 gcaaaactcc aaaaaaaaac ctgaaaaaaa cggcctgacg tgaatcaagc aatttttttc
    16621 aggttttgcc cgcttagtgc ggtaacaatc ctttattcag taataatatt tcagtgttct
    16681 ttgcgcacgc gctctatatt tatggctaaa aacataatct ctgcaggtga gattttacgt
    16741 tgatactgca aaccaataaa aatggcgatc cgctccgcac attgccatgc ttgcgggtaa
    16801 ttttgcttta ctgcttgttg taatgattca tcactatcgt taatcgatgc atgttcaata
    16861 atacgccagg ataaaaattt cagatgcgta accagtcgct gataactcaa gctttcttcc
    16921 tggtaattaa ggctgaactg aaattttatt aattgcagca tttctcgcat taattgcgtg
    16981 acacctgcaa catcctccat atttccgctc atttgggcac tgaccagatg catggcaata
    17041 aagcccactt catctttcgg taactgcacg cccaaccgtt taacaataat ggttaacgct
    17101 tcttccccta gctggaactc tttcgggtaa agtcgctgga tatcccacag cagcgggttg
    17161 ggcagtagca cgttttgctg aaagcgttta atcgcaaact ggcaatggtc agttagcgag
    17221 atataaatac tgtcctgcaa ctttcccagg cgctcctgcg ccagagagat aatacgatca
    17281 caggttgcca tcacctcaag aggcatatga cttaagagtt cgcttaatcg cccgttcagt
    17341 tcatgactgc tcaaggcata ctctttttct attccacttg agttaattct ttcgcctggg
    17401 cgtttttgaa agccaattcc gcgccccatg acgacttttt cccgctgttg atcatcaata
    17461 accaccacaa cattattgtt gagaattttg gtgatttgca tgttcatagc aaggaccttt
    17521 tttataaaca aaaaaacccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc
    17581 gaatgcagta acaatccagt cattaattaa tggtttttat aacgaacatc caggttcgaa
    17641 aattaattta gttgcgtgca gagaaaatag caatgc
//
LOCUS       DAZKJR010000105        17632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKJR010000105 DAZKJR010000000
VERSION     DAZKJR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.106"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(52..2499)
                     /gene="glgP"
                     /locus_tag="ACHOWS_003604"
     CDS             complement(52..2499)
                     /gene="glgP"
                     /locus_tag="ACHOWS_003604"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFQ0049127.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2518..3951)
                     /gene="glgA"
                     /locus_tag="ACHOWS_003605"
     CDS             complement(2518..3951)
                     /gene="glgA"
                     /locus_tag="ACHOWS_003605"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFQ0049128.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3951..5246)
                     /gene="glgC"
                     /locus_tag="ACHOWS_003606"
     CDS             complement(3951..5246)
                     /gene="glgC"
                     /locus_tag="ACHOWS_003606"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFQ0049129.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5264..7237)
                     /gene="glgX"
                     /locus_tag="ACHOWS_003607"
     CDS             complement(5264..7237)
                     /gene="glgX"
                     /locus_tag="ACHOWS_003607"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFQ0049130.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7234..9420)
                     /gene="glgB"
                     /locus_tag="ACHOWS_003608"
     CDS             complement(7234..9420)
                     /gene="glgB"
                     /locus_tag="ACHOWS_003608"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFQ0049131.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVRTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9693..10796)
                     /gene="asd"
                     /locus_tag="ACHOWS_003609"
     CDS             complement(9693..10796)
                     /gene="asd"
                     /locus_tag="ACHOWS_003609"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0049132.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10988..11581
                     /gene="yhgN"
                     /locus_tag="ACHOWS_003610"
     CDS             10988..11581
                     /gene="yhgN"
                     /locus_tag="ACHOWS_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFQ0049133.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11638..12978)
                     /gene="gntU"
                     /locus_tag="ACHOWS_003611"
     CDS             complement(11638..12978)
                     /gene="gntU"
                     /locus_tag="ACHOWS_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFQ0049134.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12982..13509)
                     /gene="gntK"
                     /locus_tag="ACHOWS_003612"
     CDS             complement(12982..13509)
                     /gene="gntK"
                     /locus_tag="ACHOWS_003612"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0049135.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13648..14643)
                     /gene="gntR"
                     /locus_tag="ACHOWS_003613"
     CDS             complement(13648..14643)
                     /gene="gntR"
                     /locus_tag="ACHOWS_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFQ0049136.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14867..15562)
                     /gene="yhhW"
                     /locus_tag="ACHOWS_003614"
     CDS             complement(14867..15562)
                     /gene="yhhW"
                     /locus_tag="ACHOWS_003614"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFQ0049137.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15685..16722)
                     /locus_tag="ACHOWS_003615"
     CDS             complement(15685..16722)
                     /locus_tag="ACHOWS_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0049138.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            17056..17544
                     /gene="yhhY"
                     /locus_tag="ACHOWS_003616"
     CDS             17056..17544
                     /gene="yhhY"
                     /locus_tag="ACHOWS_003616"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFQ0049139.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         4327 a         4804 c         4412 g         4089 t
ORIGIN      
        1 tccactctgt attacttcag cggaacacac ggtatttagc catatttgaa cttacaatct
       61 caccggatca atatgccaga tatgatcggc gtactctttg atcgtacggt cagaagagaa
      121 gtagcccata ttggcaatgt tcagcatcgc ttttgcggtc cactcttcct gacgctcgta
      181 gagttcgtcg actttatcct gacaatcgac atagctgcga taatccgcca gtacctggta
      241 gtgatcgccg aagttgatca gcgaatcgac cagatcgcga tagcgacccg gatcttccgg
      301 actgaataca ccgctgccga tttgcgtcag cacctgatgc agctcctcat ctttctcgta
      361 gtattcacgc ggtttgtagc cctgacgacg cagttcttcc acttcttccg ctgtgttacc
      421 aaagataaag atattgtcag caccgacatg atccagcatc tcgacattcg caccgtccag
      481 cgtaccgata gtcagcgcgc cgttaagcgc aaacttcatg ttactggtgc cggaagcttc
      541 cgtccccgcc agcgaaatct gttcagacag atcggccgcc ggaatgatca actgcgccag
      601 gctaacactg tagttcggga tgaacacgac tttcagcttg tcgccaatct gcggatcgtt
      661 gttgatcact ttcgctacgt cattgatcaa atgaataatg tgcttcgcca tgtaataggc
      721 cgaagccgcc ttaccaccaa aaatattcac gcgcggtacc cacttcgcat ccgggtcggc
      781 cttgatgcgg ttatagcggg tgatcacgtg caacacattc atcaattgac gtttgtattc
      841 gtgaatacgt ttgatttgca catcgaacag cgctttcgga tttaccacca cattcagctg
      901 ctgggcgata tactccgcca gacgcttttt gttctccagc ttcgcctgat gcaccgcgtg
      961 attaaccatt gggaaatcac agtgttgttg cagatcatta agcaggctaa ggtcggtgcg
     1021 ccagttgcgg cccaggtgtt cgtccaacac ggctgaaagc gatgggttcg ctaccgccag
     1081 ccagcgacgc ggcgtcacac cgttggtgac gttggtgaaa cgacccggga agattttcgc
     1141 aaagtcggca aatagcgatt gcaccatcag gttagagtgc agctccgata caccgttaac
     1201 tttgtggctc accacaaccg ccagccaggc catacgcaca cgacgaccgt tggattcatc
     1261 aatgatcgac gcccgtccca gcagatcggt atcgttcgga tactgttcct gcaaggtttt
     1321 caggaaatag tcgttgattt caaagatgat ctgcaggtga cgcggcagaa ttttacccag
     1381 catatcgact ggccaggttt ccagcgcctc gctcattagc gtgtggttgg tgtaggagaa
     1441 gacctgacaa cacacttcaa acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag
     1501 acgcatcatc tcaggaatcg acagcaccgg atgagtatcg ttgaggtgaa tcgcaatttt
     1561 atccgccagg ttatcgtagg ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat
     1621 ggtcgaggaa accaggaagt attcctgacg cagtcgcagc tcacgcccgg agtaggtgga
     1681 gtcgtccgga tacagtacgc gagatacgtt ctcggagtgg tttttatctt ccactgccgc
     1741 gaagtagtcg ccctggttga atttaccgag gttaatttcg ctactggctt gggcactcca
     1801 caaacgcaac gtgttggtcg cgtcggtgtc gtaacctggg attatctgat cgtaagctac
     1861 tcccagaatc tcttcggttt caatccagcg cgttttttta ccttcctgct gaatgcgacc
     1921 gccaaaacgg actttatagc gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc
     1981 cagccagtag tcaggcgact ctttctggct accgttaacg atgttctgct tgaacatacc
     2041 gtagtcatag cggatgccgt aaccgcgccc cggcaaccct aacgtcgcca gagaatcgag
     2101 gaagcaagcc gccagacggc ccaggccacc gttaccgagg cccgggtcat tttcttcatc
     2161 aatcagctct tcgagattta atcccatcgc ttccagtgcg ccctgtacat cttcgtaaat
     2221 tcctagcgac aacatggcgt tggaaagcgt acggccaatc aaaaactcca tcgacaggta
     2281 gtaaacctga cgagtttctt gcgacaactg ggcacggttt gagcgtaacc agcgctccac
     2341 gagacgatcg cgcacagcaa ataacgttgc gttcagccat tcatgtttat tggcgacgac
     2401 cggatccttg ccaatcgtaa acatcagctt gtaagcgata gagtgcttaa gagcttctac
     2461 gctaagcgtg ggcgatgaat atgtaaacgg agcattcata taggcgtttc ctgaaaacta
     2521 tttcaagcga tagtaaagct cacggtacga cttcgccgcg acctgccagc taaaatccat
     2581 tgccatagcc tgacgttgca caaaccgcca cagtgaagga cgggaccaca gtacaaaagc
     2641 acgtcgaata gcccgtaaca gcgaccaggc attactatct tcaaagacaa acccactggc
     2701 gacgccatct gcaaggttct caagagaaca gtcagaaacc gtatcagcaa gcccaccggt
     2761 gcgccgcact aacggcagcg taccgtactt caatccataa agttgcgtta agccgcacgg
     2821 ttcaaaacgg ctgggcacca gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc
     2881 ttcgtgatag ccaatctgaa cgcccacctg gccggggtat tccgctgccg ccgcaaggaa
     2941 accttcctgc agcaccggat cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag
     3001 aagaccaggt aaggcttcca gcaccaggtc gagacctttc tggctagtca gacggctcac
     3061 cactgcaaaa agcggcactt tatcgtcaac cttaagcccc attgcgattt gtaactggcg
     3121 cttattttcc gctttatctt ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc
     3181 cgtctctgga ctccagattt tctcgtccac gccgttcagt acgccggaaa gacgcccttc
     3241 acggtgacgc tgttgcaaca gaccttccat accgtaggca aactgcggtt cggtgatctc
     3301 gcgagcgtag gttgggctga ctgccgtaat gtgatcggca tagtacagac cggccttcag
     3361 aaaagagatt tgtccgttga attccagccc atgaatatta aagaatgacc atggcaattg
     3421 gatgtcattc atgtgatgtg cataaaacat gccttgatag gccaggttgt gcacagtaaa
     3481 caccgacttc gccggacgcc cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca
     3541 gtcgtgcgca tgcaccacat caggacgcca gaatgggtca agcccgctgg ccatttctgc
     3601 cccaacccac cccagcagcg caaaacgcaa tacgttgtcg gtataggcaa ataagttggt
     3661 atcgtgatac gggcttcccg gacgatcata gagatgcggc gcgtcaatca ggtatatgcc
     3721 aaccccgttg taatgaccga acaacagcgt gatatgtccg gcgaaggtat cacgacggga
     3781 tactacctgc gcatcggtca cgccacggcg aatatcggga aatgcaggca acagtacgcg
     3841 agcgtcaacg ccgtctgcga tttgtgctgc gggtaatgcc ccaataacat cagccagacc
     3901 gccggtttta agcagcggga acatctctga acatacatgt aaaacctgca ttatcgctcc
     3961 tgtttatgcc ctaacttccg tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa
     4021 cgatagaaac gacgtgcatc ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata
     4081 acacaagcac gatcgatgac gcagcggcgc agacggcacg agcgacctac ccatacttcc
     4141 ggtaacaata cggcggaatc aatgttgcag aatgaattca cgcgaacgcg cgagaacaga
     4201 acggactgca ccaccaccga accggagatc acacaaccgc cggataccag tgagttaagg
     4261 gtcatcccat ggctaccgga gcgatcctgc acgaatttcg ctggcggtaa tgattcattg
     4321 taggtgcgaa ttggccaatt gcgatcgtac atatccagtt ccggcaccac agaggccaga
     4381 tcgaggttcg ctttccagta agcttccagc gtacccacat cgcgccagta cggctcggca
     4441 tccgggtcgg attgtacgca agagagcggg aacgggtgcg cataggccag accggcttcg
     4501 gtgatcttgg gaatcaaatc tttgccaaag tcgtggctgg agttctcatc gcgatcgtct
     4561 tcttccagca gttcatacag atagtcggcg tcaaagacgt agatacccat actcgccaga
     4621 gatttgctcg gatcgttcgg cattgacggt gggttagcag gtttttcaac gaattcgata
     4681 attttatcgt tctcatcaac cgccataacg ccaaatgcgg aggcttcttc aatcggtact
     4741 ggcatacaag caacggtgca acgtgcgcct ttttcgacgt ggtcgataag catacgcgag
     4801 tagtcttgct tgtagatatg gtcgcccgcc aggatcacca cgtattccgc tttataacgg
     4861 cggataatat cgaggttttg ggtaactgca tctgcggtgc cgcgatacca gttttcccct
     4921 ttcattctct gctgtgctgg cagcagatcg acaaactcgt tcatttcttc attgaagaat
     4981 gaccagccgc gctgaatgtg ctgcaccaga gtgtgggact ggtactgggt gatcacgccc
     5041 atacgacgga tcccggagtt gatgcagtta gacagcgcaa agtcgataat gcggaactta
     5101 ccgccgaagt gtacggccgg ttttgctcgc ttattggtta aatccttcag gcgggtacca
     5161 cgtcctcccg ccagtatcag ggcaacagat ttcaatggca gctggcgcgc caacattaag
     5221 tgatcgttct tctctaaact aaccatgact aactcctttt ttatcatctc tggaacacac
     5281 acaatccgtg tgcaggtccc tgccagacag ccgtaatcac tgggttatcc tctccagcga
     5341 atgggggaat ggcgtgccac tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg
     5401 cgttaattgc gatcaaaaag cgatccgaga gcagaatttg cagctgtttc ggcccgtttt
     5461 gccactcatc cgtgcttaaa ggttgagcat atcgatttag ccaacggaca ttgccgtcgc
     5521 cttcttccca ccagcgattc tccaccaaag cgggaatgcg cttgcgcaga tggattaacg
     5581 cggcggtaaa tgcggttaaa ccactgcttg cctgcgacca gtccaaccag gttaattggt
     5641 tatcctgaca gtaggcattg ttattgccat gctggctgtg accatgttcg tcaccggcca
     5701 gtaacatcgg cgtaccctgg gagagcaaca acgttgttaa cagggcgtga atgctgtcgc
     5761 gacgccgttc aaccaggtca agagtaccgc ctaacccttc tttaccatga ttgttactgt
     5821 aattgttgtt ggtcccgtcg cgattttctt ctccgtttgc ttcattgtgt ttatggttga
     5881 agcaaacgca gtcgcgaagc gtaaaaccgt catgcgcggt gacgagatta atcgcggcac
     5941 tcggcagacg accattacgt ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg
     6001 cccccagagg caaatcataa tgaagccaga aacgacgggc agcatcgcgg aaatgatcgt
     6061 tccactcggc aaacagcggc gggaaatttc ccacctgata accaccagga gcaatatccc
     6121 acggttcagc aattaacttc acctgcgaga gcaccgggca gttctggata gcggtaaaca
     6181 acggcgcatc ctgacggaac tctggcgtac ggcccatgac tgccgccaga tcaaagcgga
     6241 aaccatcgac gtggcaggtt tctacccaat aacgcaggca ggcgctggca taatccacca
     6301 ccgccggatg actcaaattg agcgtgttgc cacaaccggt ccagttgtga taatcgccgt
     6361 cttctcttat ccaataatag ctacggttgt cgatcccacg cagagagaat aacgggccgt
     6421 cgaggtccag ttccgcacta tggttgagca cgatatcaag aatgacttcg atacccgctt
     6481 tatgcagtgc tttgattgca tcgcgaaact catccagcgc cgtttctggg gagcaggcat
     6541 acgccggatg cagcgcaaac atcgccaccg ggttataacc ccagtagtta cttagaccca
     6601 tacgttgcag gcgtggttcg ctggcaaact gcgccaccgg cagcagttcc agcgcggtaa
     6661 tgcccaactg tttcaaatag ttgatcatca ccggatgccc cagggcttta taagtgccac
     6721 ggatctcgac cgggatctcc gggtgcaggt acgttaatcc tttgacatgg gcttcataaa
     6781 tgatggtact gccccacggc gtgcgcggcg gagcatcatc ctcccagtca tagtgatcaa
     6841 ccaccactac gcatttcggc gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac
     6901 cggcgtgcag cagcgggtta tctttaaatt ccccgtcaat ttgccgcgcg caaggatcaa
     6961 tcaacaactt cgccgggtta aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc
     7021 gataaccata acgcaaaccc ggacgcgcat ccggcagata accgtgccaa atgtcgccac
     7081 tgtgccctgg caagtcatag cgatgttcct ggccattggc gtcaaagaca cacagttcta
     7141 cccgctcggc atgagcggag aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc
     7201 cgaggggtgc gggtttgcca atggcgagtt gtgtcattct gcctcccgaa ccagccagat
     7261 agtggccagc ggtggtagtg tcaggcttaa tgaatgctga cgaccgtggc tggcaatctc
     7321 atcgctgtgt accgtgccgc cattgccgac attactgccg tgatagtgca tggaatcggt
     7381 attgaggatt tcacgccatt tgcccggctg gttaatgccg aagcgataat catgacgcgg
     7441 caccggagta aagttactgg caacgatgat ttcgttaccc tctttatcgc gacgcacaaa
     7501 gatcagcacc gagcgttctt tgtcatccac caccagccat tcaaaaccgt acgggtcaaa
     7561 atccagttca tgcatcgctt tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg
     7621 ctggacaccg tggtgccagt tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc
     7681 atggttccac tcgcggccct gggcaaattc gttccccatg aacagcagtt tcttgcccgg
     7741 gaatgcccac atccagccgt agtaggcgcg caggttagcg aatttctgcc atgcgtcgcc
     7801 cggcatccgg tcgagaatcg attttttacc gtggaccact tcatcatgcg acaacggcag
     7861 gacgaagttt tcagtgtagt tgtagagcat cccgaaggtc agtttatcgt gatgatactg
     7921 acgataaacc gggtcgagct tcatgtagtc cagggtgtca tgcatccagc cgaggttcca
     7981 cttgtaccag aagcccagac cgcccatatc ctgcggacga gaaacgccag ggaaatcggt
     8041 agactcctca gccattgtca ccgcaccgga aacctgctca ccaagaatac ggttggtatt
     8101 acgcaagaat tcaatcgctt caagattctc gcgcccgcca aattcgttcg ggatccactc
     8161 cccctcttta cggctgtagt cgcgataaat cattgacgct accgcatcga cgcgcagcgc
     8221 atcaatacca aaacgttcaa tccagtaaag cgcgttaccg acaaggaagt tactgacttc
     8281 acggcgacca tagttgtaga tcagcgtgtt ccagtcctga tgatagcctt cgcgcggatc
     8341 gctgtgttca tacaagttcg tgccatcaaa ttcggcaagc gcaaagtcat cggtcgggaa
     8401 gtggcctggc acccagtcga gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa
     8461 ataacggaag tcgtcgcgag taccaaaacg gcgggtcggt gcatacagac cggttggctg
     8521 ataaccccaa ctgccatcga agggatgctc gttaatgggc agtagttcga ggtgggtaaa
     8581 gcccatccat ttagcataag gcaccagttg atcggccagc tcgcggtagc tcaaccagaa
     8641 attgttgtcg gtgtggcgac gccaggagcc cagatgaact tcataaatag agattggcgc
     8701 atcaaactga ttcgcttttt tgcgctcttc agtccgtaca accttttccg gcagcccgca
     8761 aataagagac gcggtttccg ggcgcatttg cgcttcgaag gcataagggt cggacttcag
     8821 acgcaagttg ccattggcat caatcatctc gtatttatag agctgaccgt tatgcgcccc
     8881 agggataaac agttcccaga tgccgctctc tttacgcagg cgcatcgggt gacggcgacc
     8941 gtcccagtag ttgaattgcc caaccaccga gacccgacgg gcgtttggag cccagacaga
     9001 gaaacgcgta cctgtgacgc catccatagt atctgcatgc gcgcctaagg tttcatacgg
     9061 gcgcaggtga gtaccttcag ataatagcca ggcatccatt tcctggatta gcggaccaaa
     9121 acggtaagga tcatcaatca ggttttgctg accatgccag acaacagcca actgatagcg
     9181 gaaaaaattc ttacgtcgcg gaatgacgcc gctaaagaat ccccgtgagt cgagacactc
     9241 cagttttgcg agtttgcgcc cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg
     9301 taaaagggca cggacttcca gtcccgcggt ggttttatgc attcccagta cggaaaaagg
     9361 atccgcaaaa tggcctgcaa ttagcgcgtt aatcacgtct ctatcgatac gatcggacat
     9421 gcttgtcttc ctgttttatt gtgtcacccc atccaattgg atttttgact tctggttgtg
     9481 acattttttt gacctgaacg gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt
     9541 caggtaaggc tgtgaatact catgtattca gccaccctta aagaatagcc aatgctctat
     9601 ttaactcccg gtaaatcatg aaacatctgc gcttactcct gtattacgca ctaacagggg
     9661 cggcatcgcg ccccagattt aatgaataaa gattacgcca gttgacgaag catccgacgc
     9721 aacggctccg cggcccccca cagcagctgg tcacccacgg taaaggctga caggaactcc
     9781 ggtcccatat tcagcttacg caggcggcct accggcgtgg tcagcgtgcc ggtaacggca
     9841 gctggggtta gctcacgcat agtgatttcc cgatcgttcg gaacgacttt cgcccacgga
     9901 ttgtgtgcag ccagcagttc ttccacggtc ggaatagaca catctttttt caatttaata
     9961 gtgaatgcct ggctgtggca gcgcaatgcc ccgacacgca cacataaacc atctaccgga
    10021 attacggaag atgtgttgag gatcttgttg gtttccgcct gccctttcca ctcttcgcgg
    10081 ctctgaccgt tatcgagctg tttgtcgatc cacggaatca ggctacccgc cagcggcacg
    10141 ccaaagttat ccaccggcag ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata
    10201 tcgagaatag cagaggacgg ggtcgcgagt tcatctgcca catggccata cagatggccc
    10261 atctgggtta ataactcacg catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt
    10321 gcaacggaca cccaatcaac aagatcattg gcgaataaac cacccaacga catcaacatc
    10381 aggcttacgg tacagttacc gccaacaaaa gtcctgatgc cattatttaa tccatcggta
    10441 atgacgtcct gattgacggg gtcaagaatg atgatggcat catctttcat gcgcagagac
    10501 gatgctgcgt caatccagta accttgccat ccgctttcac gaagctttgg atagatttcg
    10561 ttggtataat cgccgccctg acaggtcaca atgatatcga gggcctttag cgcctccaga
    10621 tcaaaggcat cctgaagtgt gccagtggtt ccgccaaaag acggcgcagc ctgaccaagc
    10681 tgagaagtag aaaagaagac agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt
    10741 tgcatgagaa cggagccgac cataccgcgc cagccgataa aaccaacatt tttcataagc
    10801 gttttttcct gcaaagatgt gtgctgtata aatgtgccgg tctcctcttg gcacatcttt
    10861 caccatacaa aaagcagcca aagtcgcaag tgaaattaat caatgatagc gaagccatca
    10921 gtaatgcgac ttatcctgct ttgttagcac gcagaaagtc cgcggcaatt atcagggaat
    10981 ttgagttatg aatgaaatca tttctgcagc agttttattg atcctgatta tggatccgct
    11041 cggaaaccta cctattttca tgtccgtact gaaacatact gaaccgaaaa gacggcgggc
    11101 aatcatggtg cgagagttgc ttattgctct cctggtgatg ctggtgttcc tgtttgcggg
    11161 tgagaaaatt ctggcatttc tcagcctgcg agcagaaacc gtctccattt ctggcggcat
    11221 cattctgttt ctgatcgcca ttaagatgat tttccccagc gcttcaggaa atagcagcgg
    11281 gcttccggca ggtgaagagc cgtttatcgt gccgttggca attccgttag tcgccgggcc
    11341 gactattctc gccacgctga tgttgttgtc tcatcagtac ccgaatcaga tgggacatct
    11401 ggtgattgct ctgctgctgg cctggggcgg cacctttgtc atcctgctac agtcttcgct
    11461 atttttacgt ctgctgggcg agaaaggggt gaacgcactt gaacgcctga tgggattgat
    11521 tctggtgatg atggcaaccc agatgttcct cgacggcatt cgaatgtgga tgaaggggta
    11581 acgaggagag tttttcgaaa agagcccggt agcgtcagaa ctaccgggta aacaaactta
    11641 actcaacagc tggaacgcaa tcatcccaac gatggcaccg acagtgccga ggatggtttc
    11701 catcatggtc caggttttca gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa
    11761 ccagaaaccg gcgtcgttaa cgtggctgac aacaatcgaa ccaccggcga tacaaatcga
    11821 cagcgccgcc atttgcgcac cagagtagtt cagttgttca atgactggca tcaccagtcc
    11881 caccgccgtt aaacaggcta cggtggcaga cccctgaatg atgcgcactg cagctgccag
    11941 cacgaagcag gtgatagcaa tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg
    12001 acctacgcca gagtcaacca gcacctgttt gaacacgccg cccgcaccaa tcaccagcag
    12061 aatgatcccc gccggttgca gcgcgtgacc gcaaatctcc atcactttgt cttttggcat
    12121 gccctgacgc attgccaggc cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa
    12181 cggatgacca ataaactcga accattcgta agcggtagag ccttctggca caaaacgcgc
    12241 ggcaatggtt ttcagcccca ccagcaccag cggcaacagg atcagcgaca ggctgaatcc
    12301 gaaagatggc attttacctt cgccgagatg cggttcgctg atgtcgtcag gaatatgcag
    12361 ctcaacgtaa cggctgatga agttacccca cagcggcccg gcaataatca ttcccggaat
    12421 tgccgcacac aggccaatca ggatcatcca gccaaagtcg gcgttcatct gcgatgccag
    12481 cagcattggc gctggccccg gcaccaggaa cgcagccgct gccgccacgc ctgcaaataa
    12541 tgggattacc agcttcacca ggttcgtacc ggtatgacgc gccattgaga aagcaacgct
    12601 aatcagcaga acaatcgcca cttcaaagaa cagcggtagc gcacaaacca gccccgcaag
    12661 gccaatggca taatgcgcgc ggctgtgacc gaaggattta agcattttga cggcaatctg
    12721 atcgactgcg ccggtttcat gtaagatctt gccaaacata gctcccaggg cgacaaccac
    12781 cgccaggaag ccgagggtgc ctcccatccc tttttccatc gtcgctgcga ttttatcgag
    12841 cggcataccg gaaaaaaggc cagcccccat ggacaccacc attaaagcca ggaaagcgtg
    12901 catacgcgcc ttcatgacta aaaacagcag cagtaaaaca gaccctactg ctgttaaaac
    12961 aagcgttaat gtagtcacta cttatttgcc ttttttaata acctcaatgg tgcttgccac
    13021 aacaccttcc agcggttgat cgatatccac caccagtaca tcggtttcgt ccgcacccgg
    13081 ctcctgcagc gtttcaaact gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg
    13141 cgctttcagg cggctttcaa tcacatcaaa atcacctttc aggtagatga aagagagatt
    13201 cggattacct tcacgcagca agtcgcgata gtgttttttc aatgcagaac agacgatcag
    13261 tgacacttta ttagtgcgct gcatagcaaa cgcggcgtcg ttcagcgcct gcagccacgg
    13321 tttgcgatcg tcatcattca gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg
    13381 atggaggaaa tcgccatcaa gaaacgcggc atgaagttga tgcgccactt cacttgcgac
    13441 cgcagatttg ccgctgcccg atacgcccat caagacgtaa atgtggtgat catggttagt
    13501 cgtgctcaaa gtggtgcccc cacaatacaa gaattaaaaa tgttacgggt aactgttatc
    13561 ggtaacattg tccagccgga caatatcaga agcaatatcc atccgtgcct taagtgtata
    13621 agtgtgagct acttcaaatt tgtgggctta aatagatccg cccggtgaca aggtgaaacc
    13681 taaatctaac attttcggtg tcacagattc gccacgaata cgcgccagca ggcgttcagc
    13741 gccaatactg cccatccgct cacgcggcgt caggacgctc gccaaccgtg gctccatcac
    13801 ctggccaatg tcatgaccgt ggaaaccggc aatcgccata tcgtcaggaa ctttcaaccc
    13861 cagacgctga cattcaaacg ccgcgccgac cgccaggtca tcattagtac agaacacgcc
    13921 atccagctgc ggatattccc gccgtgcctg gcgaatcagt tcaataccgg aagaataaga
    13981 agaagattgc tcaaccatca cgctatacgg caccaggcct gcatccagca tcgcctgttc
    14041 atatcccttc tgtttgatga tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg
    14101 gcgatgcccg cgagcaataa tggctgtggt catctggcgt gccgcttcaa agttatcaaa
    14161 accgacggcg atatcgaggc aaggcgactt gctgtccatc agttccacca ccgggatccc
    14221 cgccacttca atcatcttta aggtgcgcgg cgtgtgggta cgttcggtga ggatcagtcc
    14281 gtcgatattc catgagagca tggattcgag acgttcttgc tccatttccg gtttataacc
    14341 gtagtgggcc agcatggtct gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa
    14401 tacttccgcg aaaacctggt tggtgagaga aggtaacagg acgccaatcg cccggctggt
    14461 ggcgttagag aggatatcgg gtgcgcgatt gggaatataa cccagttcat caagagcggc
    14521 ggcaatcttg ccgcgtagag cgacggaaac ctgctccggg ttacgcaaaa agcggctgac
    14581 cgtcattttg gtcacgccta cacggtcagc cacatcctga agtacgggtc ttttcttttt
    14641 catcgtcctg aaggtacaaa agagatagat tctcttagtt taacacggac gcatcagaac
    14701 cttcccgggg aaaacagtgc ttgtttacaa ttattagata tacactaaat caattgcatc
    14761 gcattgtgct aacgacgcgg caaaaccccg gaagcgtaca tcagcacgcg accggagcga
    14821 gcgaggaagc ctgcaaaaag gcaggcttca aagatgccgt tgagctttaa accggcggca
    14881 gatcgaacag taacacttcg ctgtcgctat ccgcatggat ggagattgcc tgctcatccc
    14941 agattgccag accatcgctg atcgaggctt tcacgccgtt aatggtgaca ttgcctttca
    15001 ccacctggat ccagacgcgg cgttcagcgg caatctgatg caccgactgc tcatctttca
    15061 gcaacgccca gcggtacagt tccatatcct gatgcacttt caatgaacca tcacgcgcat
    15121 ctggcgaaag caccagttgt ttgccctgca ctgcatcgaa gcgacgctgt tcataacgcg
    15181 gcgtaatgcc gttttcttcc ggcatgatcc agatctgata caggtgcaga cgctcggtgc
    15241 tgcttgggtt gtactctgag tgacgaatac ccgtacccgc actcataatc tggaactcgc
    15301 ccgccggaac ctgctcttta ttgcccatgc tgtcctggtg ctcaacagta ccttccagca
    15361 cgtaggtcaa aatttccata tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa
    15421 tcacgtcgtc gttaatcacg cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt
    15481 tggcaaaaga gaaagtatgc caggagtcca gccagccatg atttgcatga ccacgttcat
    15541 ttgctttgcg taagtagatc atttgtattc gccccctgaa tgatttcgat gggcttagtg
    15601 tggacccgat ctgcctggga tgatagaggg tgaaaattga cccctctgtt caaaaaaatt
    15661 gaactattcg aggggccatc aaacttactt cgcgagagtc actgtggagg gagaggcttg
    15721 ctcaaatccg cgttcaagga tttccagatt ggtaagaact tcagattcct tgacgtaatt
    15781 tggcgcaccg ttggtgatgg tttgatacaa cgcatcataa acgcgcccgt aatcgcccat
    15841 ctccggcttc atctcttctc tgaccgtcac accctcgtca ttgacatact ccagcacacc
    15901 gaccgaatca tccgctgcga atcccggttc gcccggcata atattagcct tgaggctggt
    15961 ttcctgctgg tcgataccgt atttaataaa cgaacctttc ttaccgtgaa cgataaattt
    16021 cggataatcg attttcacca gatggctggt tttgacgatg gctttcaagt cgccataaaa
    16081 cagttgcgct tcaaaggtgt cgtcaggatt ggctttattg cgcaggctgc ggatgtcata
    16141 agcgacgtga tccgggcgac cgaacagaga aataatctgg tccatcgtat gcacaccaag
    16201 gccatagaac gcgccatcct gcggcaaccc aggtttggtt tctgccaccg ggcggtaata
    16261 gtcaaaatgg ctttccactt caacaatctc tcccaacttg ccgctttcaa tcgctttttt
    16321 cgccgtcagg aagcaggaat caaagcgacg attctgatac ggcgtgacgg tcagcccttt
    16381 gcttttcgcc agcgcaaaca gctccttcgc ctgggcaagc gtcggggtga acggtttttc
    16441 gaccagcaca tttttcccgg cttccagcgc gcgtttcgcg tattcgaaat ggctgtccgc
    16501 gtgggtgcag acgacaacca gcttaacatc gggatcgttt agcacttcgt cgagatcgct
    16561 ggtgaaatgg atatgagagt aaatgggagc ctgttcttcc ggctttgcat ggcggcgaaa
    16621 aatatgcgcg acatgccagc tatccttgcg gttaagtaca tacggcagat ggtaacgggt
    16681 ggtgcttttg ccgaagccaa taaaggcgca gttgatgacc atgatttcgt cctttttaag
    16741 gtggttattt acaccttagc gcaaagcgga cgtggttcct actggagtgc gcataacgaa
    16801 cacaagcact tcccgaggat aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg
    16861 ctggctaagt aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg
    16921 gtcttcctga tcgcgagaca ataataatca ttctcattcg cacttgtcca acactttttg
    16981 caaaaaaatg catttgactc gcattcgaaa gtcaatgatg ttgaaaggga catttacccc
    17041 aaagaggaca aaggaatgag tgagatagtg atacgccacg cagaaacacg ggattacgag
    17101 gccatcaggc agattcacgc ccagccggag gtgtattgca acacactaca ggtgcctcat
    17161 ccctccgatc atatgtggca ggaacgactc gccgatcgcc ccggcatcaa gcaactcgtc
    17221 gcctgtattg atggaaccgt agtaggtcat ctcaccattg atgtacagca acgcccgcgc
    17281 cgcagtcatg ttgccgattt tggtatctgt gtcgactccc gttggaagaa ccgcggcgtc
    17341 gccagcgccc tgatgcgaga gatgattgaa atgtgcgaca actggttgcg ggtagatcgt
    17401 attgagctaa cggtgttcgt cgataacgcg ccagcaatta aggtctataa aaaatttggc
    17461 tttgagattg aagggactgg caagaagtac gcattacgta atggtgaata tgtcgatgcg
    17521 tattatatgg cgcgggtgaa gtaagatagt gccctttttc tgagatggaa aaagggtgtc
    17581 attcaaaatc ggcatacctt cctttaaggt atttattttc ccaatacata ta
//
LOCUS       DAZKJR010000106        17428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKJR010000106 DAZKJR010000000
VERSION     DAZKJR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.107"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(206..1135)
                     /gene="fdhE"
                     /locus_tag="ACHOWS_003617"
     CDS             complement(206..1135)
                     /gene="fdhE"
                     /locus_tag="ACHOWS_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFQ0049140.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1132..1767)
                     /gene="fdoI"
                     /locus_tag="ACHOWS_003618"
     CDS             complement(1132..1767)
                     /gene="fdoI"
                     /locus_tag="ACHOWS_003618"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFQ0049141.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1764..2666)
                     /gene="fdoH"
                     /locus_tag="ACHOWS_003619"
     CDS             complement(1764..2666)
                     /gene="fdoH"
                     /locus_tag="ACHOWS_003619"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFQ0049142.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2679..5729)
                     /gene="fdnG"
                     /locus_tag="ACHOWS_003620"
     CDS             complement(2679..5729)
                     /gene="fdnG"
                     /locus_tag="ACHOWS_003620"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5142..5144),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFQ0049143.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKVYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5923..6756
                     /gene="fdhD"
                     /locus_tag="ACHOWS_003621"
     CDS             5923..6756
                     /gene="fdhD"
                     /locus_tag="ACHOWS_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFQ0049144.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRHDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            6845..7535
                     /locus_tag="ACHOWS_003622"
                     /pseudo
     CDS             6845..7535
                     /locus_tag="ACHOWS_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
     gene            7889..9283
                     /locus_tag="ACHOWS_003623"
     CDS             7889..9283
                     /locus_tag="ACHOWS_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFQ0049145.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATSESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            complement(9324..9638)
                     /gene="rhaM"
                     /locus_tag="ACHOWS_003624"
     CDS             complement(9324..9638)
                     /gene="rhaM"
                     /locus_tag="ACHOWS_003624"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0049146.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPTNPDNSPVSSELQEVFY
                     LP"
     gene            complement(9648..10472)
                     /gene="rhaD"
                     /locus_tag="ACHOWS_003625"
     CDS             complement(9648..10472)
                     /gene="rhaD"
                     /locus_tag="ACHOWS_003625"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0049147.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNKAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            complement(10657..11916)
                     /gene="rhaA"
                     /locus_tag="ACHOWS_003626"
     CDS             complement(10657..11916)
                     /gene="rhaA"
                     /locus_tag="ACHOWS_003626"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0049148.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKTNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            complement(11913..13382)
                     /gene="rhaB"
                     /locus_tag="ACHOWS_003627"
     CDS             complement(11913..13382)
                     /gene="rhaB"
                     /locus_tag="ACHOWS_003627"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0049149.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTHGLMAQAQRQLGKCDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQSNEIPVVAVASHDTASAVIASPLNSSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            13670..14506
                     /gene="rhaS"
                     /locus_tag="ACHOWS_003628"
     CDS             13670..14506
                     /gene="rhaS"
                     /locus_tag="ACHOWS_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFQ0049150.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARYLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            14580..15428
                     /gene="rhaR"
                     /locus_tag="ACHOWS_003629"
     CDS             14580..15428
                     /gene="rhaR"
                     /locus_tag="ACHOWS_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFQ0049151.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(15425..16459)
                     /gene="rhaT"
                     /locus_tag="ACHOWS_003630"
     CDS             complement(15425..16459)
                     /gene="rhaT"
                     /locus_tag="ACHOWS_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFQ0049152.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            16744..17364
                     /gene="sodA"
                     /locus_tag="ACHOWS_003631"
     CDS             16744..17364
                     /gene="sodA"
                     /locus_tag="ACHOWS_003631"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFQ0049153.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
BASE COUNT         4182 a         4481 c         4515 g         4250 t
ORIGIN      
        1 tattattctt gaatataaaa accagatgcc cttattctgg tattaataca agactgtttt
       61 acttgaactt ataataactg taactgttac atcatatctg gaaaacgcct cgcaaaatac
      121 gagagatttt gcgggaatag caggtgtcgt cacaccctat gagctgtaat ccgggcgacc
      181 agcgccaccc ggaaaacgtc agagattact ccccttcacc cgggaacagg aacgggttga
      241 tggaactgcg ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt
      301 catctgccac ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc
      361 cacagtcatc gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat
      421 gcagtttgcc gctctgctcg cagttgctgc attttacgcg caccacgtgc cattcggttt
      481 cacacaggtt gcaatgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg
      541 acaccggcat agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg
      601 ctttgccggg gatcagatta gccatctgcg cccagtagag cgacagcgca gcccagataa
      661 acggcgcttt atcgctgctg acggacgaga aatcagaggc aaacagtgcg ctggccatat
      721 cttccagctc ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc
      781 tcatttcagg tttcagctca gcaatcagcg ccatcagcag cttttgccag tgcttatcac
      841 gcggcagaac gtgaatatcc agcgggggct tgccttgtgc gctggcttct ttaatgcgcg
      901 tggtcagatc catctccagc ggatggtcgt acagcaccac ttcctgggcg tgggcgataa
      961 gcgcagcaaa gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct
     1021 cggcgcggcg gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg
     1081 ccgtacgttt ctcgctcgaa cccagctcat cttgcgggat tatgcgaata ctcattcagc
     1141 tttcttttcc gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca
     1201 ggcgctggta acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc
     1261 gtagatatgc accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa
     1321 cgcgaatcgg atcaccggga ttgagaaagc aggtgcaaaa taaggacgcc agataatcac
     1381 gccgctcacc agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc
     1441 gaagttataa cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt
     1501 cgcccaaaag atatcatccc gattgattag gttgtgatgc cagtaacgga aaaacatgat
     1561 gatgaacgag gcaaacataa ccacgccgac aaacgggtgc agaatgcgcg ccagctgcgg
     1621 tgtgcccatg atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac
     1681 cgccgccagg atgaagcaga aggcggtgat ccagtggttg atacgttccg gcgcggtgta
     1741 gcgcacgatg gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta
     1801 ttctcttcct catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca
     1861 aaggtagccg caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg
     1921 gtttcgctga tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg
     1981 tgcagcacgt acataacatg tgtaccaccg acgcctgccg ggtcgtacaa acccgcattg
     2041 tcgtaaccac gggttttcag ctccgccacg cgctcgctcg ccagcgtttt catcgactct
     2101 ttcgtaccga agtgaatcgc gcccgttggg caggtcttca cgcaggccgg ttcttgccca
     2161 accaccacgc ggtcaacgca caacgtacat ttgtagacgc ggttgtcttc cgggttgagg
     2221 cgcggaatgt cgaacggaca gcccgcaatg caataaccgc agccgatgca ctgctcggac
     2281 tggaagtcga cgataccgtt ggcatactga ataattgccc cttccgccgg gcacgctttc
     2341 aggcagcctg gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg
     2401 tcgttctgct ccacttccga gaatcgcatc accgtccacg atttggcact taaatcattg
     2461 gggttgtcgt acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa
     2521 cacgccacct gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact
     2581 tcttcctgga agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga
     2641 atgatatctt gcgattgata agccatatgt cgtctccgtt acaccttttc cacattcaca
     2701 aggaaggact taaactccgg cgtctgcgtg ttcgcatcac cgacgaatgg cgtcaacgta
     2761 ttggcaataa agcctttttt cgcaacacct tcatagcccc agtgaatcgg aataccgatg
     2821 gtatcgatat ctttgccgtt tgctttcagc gtgcgaatac gtttggtcac taccgctttg
     2881 gctttgatat agccacggtt ggaggagact ttcacagtat cgccctgggc aatgccaagt
     2941 ttattcgcca gcgactcccc gatttccaca aactgctctg gttgcaaaat cgcattcaac
     3001 agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac
     3061 gggaacttat cggctttacc caatgcttcg gcgtcgtctt taaagatacg cgcagccggg
     3121 ttcgagataa cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag
     3181 tgttccggga acggaccttc cgccatctta tcgagggcaa acagacgtcc catgccttcc
     3241 tgctgcatga taaacggccc tacgccgctg cccggaggcg ctgcgctgta gtccggaata
     3301 tcccagccgg tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccatggg
     3361 ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc
     3421 catgcccagc ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc
     3481 tggttgcctt ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg
     3541 tcatcgcgca gttgagcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg
     3601 gttgccggat cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc cacttcttct
     3661 gaagatggct catgcggaat ggcgtagttc caggtcatgt tcagcacctg gtccgggttt
     3721 gcgccaccct gttctgcata catcttgcgc aagcgcagga agataccgga gaggatctcg
     3781 ccatcagtca gcgcaatccc tggggcgtcc gcacctttcc agtgccactg taaccagcga
     3841 ccggagttaa cgattgaacc gttctcttct gcgaagcaag tcgatggcag acggaacact
     3901 tcggtctgga tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta
     3961 gaggtttcag tgttcagcgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg
     4021 atcactttgt ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca
     4081 ttgaccttgc cttctttcat catctcgaag tattgcagga cgtcgtagac tttatcccac
     4141 ttcggcaacc aatcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc
     4201 ttcatcatag agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc
     4261 agcggttttg gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgctcggc
     4321 agcgtcatgt aacccggcag gctctgcgac agcagcccca ggtccgtcag cccctgaata
     4381 ttggagtgac cgcgcagggc gttaacgccg ccgcctgcca tccccatatt gccgagcagc
     4441 aactggatca tcgccatcgt acgaatgttt tgcgcaccaa tggagtgttg cgtccagccg
     4501 agcgcgtaaa ggaacgaggc agttttatcg tgagcactgg tctcagcgat gtattcgcag
     4561 actttcagga atgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag
     4621 cgggaaacgt gctgtttcag caagttccac acgcaacgtg gatgttgcag ggtagtatcg
     4681 cgtttggcga agccgttttc gtccagttcg taagtccagg aggatttatc gtacttacgt
     4741 ttttccgcgt catagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc
     4801 aggctggcgt tggtataggc ttcggtgtat tcgcggttga atttttcatt gttcagcagg
     4861 tacagcaata cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag
     4921 tcagctaccg ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccattg
     4981 tgaattttgg cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg
     5041 cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg
     5101 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg
     5161 tcgaccgcga gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta
     5221 ctcgacgcgg aagcacacag catcccggtg gagagccagc ggttaacggt cacgccttcg
     5281 gcgttttgcg caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca
     5341 aacgcctctt cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga
     5401 aacttcagac ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt
     5461 gcaccgcggt tgaccgggtg atccgggtca ccttcgatat ggaagataga tgctttggcg
     5521 ttttttgctc cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg
     5581 caggtattac gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct
     5641 acgctgggtg caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc
     5701 ttaaagaact gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc
     5761 ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg
     5821 gccgcaaagg tccagaattg gctcaatttc cctccatcca ggaagggttt gtaacagaat
     5881 accataatgt tggtgtgtgt gttcttatct ggttaagaga aagtgaaaaa aacacagcaa
     5941 aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcat
     6001 gatgatttac aacacccacg gttagatgag gtggcggaag aagttcccgt tgcgctggtc
     6061 tacaacggta tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg
     6121 ctcggttttt cgctttccga ggggataatc gaaagtccgc gcgatatctt cggcatggat
     6181 gtcgttcctt cctgtaatgg tcttgaagtt caaattgagc tttccagccg ccgctttatg
     6241 gggttgaagg agcgtcgccg ggcactggcg ggacgtacgg gatgtggcgt gtgcggcgtg
     6301 gagcaactta atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat
     6361 ctcaacaaac tggatgatgc attacgtcat ctcaatgatt ttcagccagt ggggcaactg
     6421 accggttgta ctcacgccgc cgcctggatg ttgccatctg gcgaactggt cggcgggcat
     6481 gaagacgtgg gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg
     6541 gaaagctggc agcaaggtgc ggtactggtt tccagccgtg ccagttatga aatggtgcaa
     6601 aagtcggcga tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacacttgct
     6661 gtagaagtgg ctgaacgctg taatttgacg ctggtgggct tttgtaaacc gggtcgggca
     6721 acggtttata cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata
     6781 ttaaattgca ggacttattc atttcgtgaa ttttattatt ttatttataa acaaaacgcg
     6841 tgtagtgtcg ttaacgcctg cgttgcaacc tattgatggc gtggcggtga gttatattga
     6901 tgctgccgta gcgttgggta ataccattaa cgaaatggat aaatattaca tccaggaaaa
     6961 ttataaagac gatgcgtttg cgaaagggaa aacgctgcac cagacattct taaaaaatct
     7021 ggaagccttt gaacctgtag cagaatctta tcatgcggcg attcaggaaa ttaatgacaa
     7081 gcgccagctt acggaattga aaaatattga agaaagagaa gggaaaacat tccactacta
     7141 ctctctggca gtcatgattt cagcgaaaca aattaataac ctgatatcgc aagataagtt
     7201 tgatgcagaa gcggcaatga agaaagtgtc tgaactggaa acgctggtgg cgcaggccaa
     7261 agaagcggat aaaagcggca tgaatttctc gtttattaat tcggcaggcc agtatcaact
     7321 cgaggctaaa aaatacgttc gccgcatcag agataaagtc ccgtactctg actgggataa
     7381 agagcaactt caggacgcaa actcaagctg gatggccgaa gactcatttc ccgagagcat
     7441 tatgcgagta caacgaaatg gtcgatgaaa tatttcagtc aattgtaatc gcggagcggg
     7501 tttgtgatga gcgcggatat gtcacgaagt cgtgacaata taaagcagag tgaaggataa
     7561 attttgtgaa taaagtatca ttatttaatg attttgtgaa tgtttaaatg tctcagataa
     7621 tttgtttaag cgttatttat atttgtggct tatgagtcag cgtgaatcat atctaatatt
     7681 ttttacgata tttagttaaa aacggcacta ttgttatatt cctcacgttc caggaaatca
     7741 gcggatttac tcatattata tggcgggata ctatccatcg gcctgctgaa gttggattaa
     7801 aaattcgaaa gtgcacacct tatatttctc tatatgcgta ttccttttgg aaagcgtagg
     7861 ggctctttta tttcagggaa cgtaaataat gaaaaaatcg acattatctt tagccatcgg
     7921 tttattattg gcatgtagta ccggaatggc aaaaacacag catttaacgc tggaacaacg
     7981 catggcattg ctggaagaac gcctggaagc ggcagaaatg cgggcagcaa aagcagagag
     8041 ccaggttaaa cagctgcaga cacaacaagc cgctgagatc cgcgaaatta aggctgccca
     8101 gggcaatacg ccggtaaatg gacaggcaac gtcggaatct gcaaagaaaa attccacctc
     8161 acctaatctt ttgctttcag gttatggcga tttaaaaatc tacggcgacg tagaatttaa
     8221 tatggatgcg gaaagtaatc atggccttct ggcaatgacc aacgctgatg tgaatagcga
     8281 tcccactaat gaacagtgga atctcaatgg tcgtattctg ttaggttttg acggtatgcg
     8341 aaagatggac aacggttatt tcgccgggtt ctccgcacaa ccgctggggg acatgcacgg
     8401 ttcagtaaat atcgatgatg cggtattctt ctttggcaaa gaaaacgact ggaaggtcaa
     8461 agtaggccgt tttgaagcct acgatatgtt cccgctgaat caggatacct ttgttgaaca
     8521 ttccggtaat actgcgaacg atctttatga cgatggcagc ggttatatct atatgatgaa
     8581 agagggccgc gggcgttcta acgctggcgg taatttcctc gtcagcaaac aactcgataa
     8641 ctggtatttt gaattaaaca cgttactgga agacggaaca tctttatata acgacggtaa
     8701 ttatcatgga cgcgatatgg aacagcagaa aaatgttgct tatctgcgtc cggtaattgc
     8761 ctggtcgccg acggaagaat tcaccgtttc cgcagcgatg gaagcgaacg tggtaaataa
     8821 tgcttatggt tataccgata gcaagggtaa ttttgtcgat cagtccgatc gtaccggcta
     8881 tggtatgagc atgacctgga atggcctgaa aacggatccg gaaaatggtg tcgtggttaa
     8941 tcttaatacc gcctatttag atgctaataa tgagaaagat ttcactgccg ggattaacgc
     9001 gctgtggaaa cgtttcgagc tgggttatat ctacgcgcac aataagattg atgaattcag
     9061 cggtgtagtt tgtgataacg actgctggat tgatgatgaa gggacgtaca ccattcacac
     9121 cattcatgcg tcttatcagt tcgctaatgt gatggatatg gagaacttta atatttacct
     9181 cgggacgtat tactccattc tggatagcga cggtgataaa aaacacggtg atgatactga
     9241 tgaccgttac ggtgcacgcg ttcgctttaa atacttcttc tgatgaagtc gcggtgctgg
     9301 atattttccg gcaccgcaac tcattaaggc aggtaaaaca cttcttgcag ctcgctactg
     9361 actgggctgt tatccgggtt agttggcata acgtcggtca tatatttcca ccaacgttgg
     9421 caaacatcag tactggctac cgcattccac cgttcttcag attcaatctc taccgtggca
     9481 aacagcagat tacgcgcttt gtcgaggtaa atggcgtagt tatgcgcacc gtgagatttc
     9541 aacactgctt ccagttccgg ccagattgga ttatgacgac gctgatactc ttcgtgggcg
     9601 tcggggttaa cctgcatgac aaaggctttg cggatcatgt ttgctcctta cagcgccagc
     9661 gcactggcga gtggtgtaac gccgaaacgc tggccgagcg ctatcagctc ttcacgactg
     9721 atggtctgtt tcatgccgcc catcgaataa accttcacta atacttgtgc tgatttttct
     9781 gcggtgtcga ttaaaccgaa tgtttcatcc agtgtcggac cgctgccgaa gacgccgtgg
     9841 aagggccaca acaccagcga atgtttttgc atttcctgtg cggtcgcctg gccgatttcg
     9901 tcggtgccgg gcaccatcca cggcaaaatg ccgacgccat ccgggaacac taccagacat
     9961 tcggtgctgc cttcccacag ttggcgagtg aagaccgcgg tgtcgttttc cagtacgtag
    10021 gtaagggcga tcaggttggt ggcatggcag tgcatgatca cccgatcttt accgttggtg
    10081 gctttaatgc gctcgcagtg ggagagaaag tgagccggaa gttcggaagt ggggacggct
    10141 ttgttggtta acccccaaag aatgtggtag cccgcgccgt cgctgtcgac ttttacgatg
    10201 cctaagttag ccgcaggatc aagctggacg ttacggaaga atttgcctga gccggtgaca
    10261 ataaacggtg tatttgccag taaaggcatg ggctggctga gcgggatata gcgcggttgt
    10321 tggtgaaaat tgtcgtgata tggtgcgata tcggcgtcat ccaggcgcag cgtcaggttg
    10381 ccgccgttgc gctcgtccca gcctttcagc caggcgtcgg tggtggcttt aatcattccc
    10441 tggacaaacc aggactgagt aatgttttgc atgttcggtg ttcctgttaa ttggacgtcc
    10501 gtgcctgttg ccggatatac gatctgtagg ccggataagg cactcgtgcc gcatccggca
    10561 atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct acaactattg ccgacccgta
    10621 ggccggataa ggcacttgtg ccgcatccgg cagtgtttac ccgcggcgac tcaaaatttc
    10681 tttctcataa gcccgcacgt tctccagcca ttcgctaccc gctggcgtat cgtgacgctg
    10741 acaatacatt tcccataccg cctgccacgg caacgatttc tgctcttcca gtagcgccag
    10801 acgcgcagtg taatcgcccg ccgattccag cttgcgtagt tcagcggtag gttccagtaa
    10861 cgcacgtagc agggcttttt tcatattgcg tgtaccaatg acccacgcgg caatgcggtt
    10921 gatagaggca tcgaagaagt cgaggccgat atgcacccgg tcgaacagat cgtgacgaac
    10981 aatctcactg gcaatcgcct gggtttcatc atccagcagc actacgtgat cgctgtccca
    11041 gcgaaccgga cggctgacgt gcagcagcaa ctgcggcaca tacagcatgg cggcggaaat
    11101 cttgtcggaa atcacttcag tcgggtggaa atgcccggcg tccaggcaca gcgccgtctg
    11161 gcggctggtg gcatacccca ggtaaaactc attggagcca accgtgtagc tctccgcgcc
    11221 aatgccaaac aatttgctct caacggcatc gatatgatgc gccggatcca gcttctcgct
    11281 gatcacctca tccagtgctg ccagcagacg ctggcgcggg gcgagacggt caacggtgat
    11341 atctttcatg ccatccggga tccagatgtt catcaccgac ggtgtgccga gttgctcgcc
    11401 aaaataggct gaaacacggc ggctggcctt gcagtgatca atccagaact ggcgaatact
    11461 gtcgtcggca tgggaaagcg taaagccatc ggcgcttagc ggatgcgaaa aacaggaggg
    11521 gttaaaatcc agaccgagct gattggtttt cgcccattca acccagtttt tgaagtgctc
    11581 tggtttgatt tggtcgcgcg cgactggcgt atctgattcc agatagatgg catgtaaatt
    11641 aagccgtttc ggccccggaa tcagccgcat agcttgttcc agatcggcac gtagctcgct
    11701 ggcattacgc gctttgcccg gataattacc agtagcctga atcccaccgg tcagcgaacc
    11761 ttccgggttt tcaaaaccgg aaacatcatc gccctgccag cagtgcattg aaacgggtaa
    11821 acgatcaagt tggcgcagcg cctcctcgac atcaatccct actgccgcga aacgctgttt
    11881 cgccagctcc caggcctgtt ccagttgagt ggtcatgcgc aaagctcctt tgtctgtcgt
    11941 gtagagtgaa tctgcgccac atagtgggca atttcactgt caggattagg ggtaaaggtg
    12001 gtcagattcg cggtggtgct gacgacctga cggaaatcat ccacattgtt gagttcatcc
    12061 agcgtcatta actggatgcc gatattgccg agcgtcgagg cttcaacagg cccggcgatc
    12121 acccgaatac cgcaggcatc agcacataac tggttgagca gcgcgttctg gcagccaccg
    12181 ccgacaatat gcagttgtga gaaatcttca ccgcgcagtt gcgccagctc atgcaacaca
    12241 tcggcataca gcagcgccag actgtcgaaa atacagcgcg ccagttcagc atcactttcc
    12301 gggattggtt gcgccgtttc ccgacacgca gcctgaattt cgctgcacat ctcgtcagga
    12361 ttaataaagc gatcgtcatt gggattgatg gtgaagcggc aagccggaag tgcctgtgtc
    12421 gcggcgataa gcgccggaag atcgttgatt tgccgctcct gaagcacccg ctgaagcagc
    12481 cataagccca taatattttt cagcacccga tagcgacctt ccgccccgcc ttcattggtg
    12541 atgttggctg ccagcgccgt gtcattggta aatggcgtct ggctttcgaa gcccatcaat
    12601 gaccaggtgc cagaagagag ataagcggcg cgtgaactgt ttaacggcga ggcgataacc
    12661 gcgctggcgg tatcatggct ggcaacggcg accaccggaa tctcattact ctgcgggcaa
    12721 atccagtgcc caatgacatt acccggatgc gtcgggcgac caaaccaggc tttgttggcc
    12781 ccgctccacg ccagtagcga ctcgtcccag tcgtcgctat tgatattgac cagttgcgtg
    12841 gtcgtggcgt tggtatattc ccagttcatc ttgccggtca gacgataact gaagtaatcc
    12901 ggcatcagca gagcgtgagc aatgtgtgga ataagttcag gttgttgctc cgtcagcgca
    12961 cgcaactgat aaagcgtatt gaagggcaaa aactggatgc cgctacgttg ataaatatcg
    13021 catttgccga gttgccgttg tgcctgcgcc atcagaccat gggtgcggct atcgcgataa
    13081 gcaacaggca ggcccacgcg ctgaccctgt tggtcaagca gcacaaagtc cacgccccag
    13141 gtatcaatcc caatgctatc gatacgaatc ccttcctcgc acaccttgtt taatccaagg
    13201 cgaatggcac tttccaggct atccacatcc caggtgacat aaccgttctg gctatgcagc
    13261 ccattgttaa aacgatggat ttcgcgcagc gtcaggctgc ggcattcacg ctcgtaacgc
    13321 gccagcatca cgcgtccact ggatgcgccg agatcgacgg cgacacaatt gcgaaaggtc
    13381 ataatgtgat cctgctgaat ttcattacga ccagtctaaa aagcgcctga attcgcgacc
    13441 ttctcgttac tgacaggaaa atgggtcatt ggcaaccagg gaaagatgaa cgtgatgatg
    13501 ttcacaattc gctgaattgt ggtggtgtga tgctcaccgc atttcctgaa aattcacgct
    13561 gtatcttgaa aaatcgacgt tttttacgtg gtttcccgtc gaaaatttaa ggtaagaacc
    13621 tgacctcgtg attactattt cgccgtgttg acgacatcag gaggccagta tgaccgtatt
    13681 acatagtgtg gatttttttc cgtctggtaa cgcgtccgtg gcgatagaac cccggctccc
    13741 gcaggcggat tttcctgaac atcatcatga ttttcatgaa attgtgattg tcgaacatgg
    13801 cacgggtatt catgtgttta atgggcagcc ctataccatc accggtggca cggtctgttt
    13861 cgtacgcgat catgatcggc atctgtatga acataccgat aatctgtgtc tgaccaatgt
    13921 gctgtatcgc tcgccggatc gatttcagtt tctcgccggg ctgaatcagt tgctgccaca
    13981 agagcaggat gggcagtatc cgtctcactg gcgcgttaac cacagcgtgt tgcagcaggt
    14041 gcgacagctg gttgcacaga tggaacagca ggaagaggaa aatgatttac cctcgaccgc
    14101 cagtcgcgag atcttattta tgcaattact gctcttgctg cgtaaaagca gtttgcagga
    14161 gaacctggaa aacagcgcgt cacgtctcaa cttgcttctg gcctggctgg aggaccattt
    14221 tgccgatgag gtgaattggg atgccgtggc ggatcaattt tctctttcat tgcgtacgct
    14281 gcatcgccag cttaagcagc aaacggggct gacgcctcag cgttacctga accgcctgcg
    14341 actgatgaaa gcccgatatc tgctacgcca cagcgaggcc agcgttactg acatcgccta
    14401 tcgctgcgga ttcagcgaca gtaaccactt ttcgacgctt tttcgccgag agtttaactg
    14461 gtcaccgcgt gatattcgtc agggacggga tggttttctg caataacgcg aatcttctca
    14521 acgtatttgt acgccatatt gcgaataatc aacttcgttc tctggccgag gtagccacgg
    14581 tggcgcatca gttaaaactt ctcaaagatg atttttttgc cagcgaccag caggcagtcg
    14641 ctgtggctga ccgttatccg caagatgtct ttgctgaaca tacacatgat ttttgtgagc
    14701 tggtaattgt ctggcgcggt aatggtctgc atgtactcaa cgatcgccct tatcgcatta
    14761 cccgtggcga tctcttttac attcatgctg acgataaaca ctcctacgct tccgttaacg
    14821 atctggtttt gcagaatatt atttattgcc cggagcggct gaagctgaat cttgactggc
    14881 agggggcgat tccgggattt agtgccagcg cagggcaacc acactggcgc ttaggtagcg
    14941 tggggatggc gcaggcgcgg cagattatcg gtcagcttga gcatgaaagt agtcagcatg
    15001 tgccgtttgc taacgaaatg gcagagttgc tgttcgggca gttggtgatg ttgctgaatc
    15061 gccatcgtta caccagtgat tcgttgccgc caacatccag cgaaacgttg ctggataagc
    15121 tgattacccg gctggcggcc agcctgaaaa gtccctttgc gctggataaa ttttgtgatg
    15181 aggcatcgtg cagtgagcgc gttttgcgtc agcaatttcg ccagcagact ggaatgacca
    15241 tcaatcaata tctgcggcag gtcagagtgt gtcatgcgca atatcttctc cagcatagcc
    15301 gcctgttaat cagtgatatt tcaaccgaat gtggctttga agatagtaac tatttttcgg
    15361 tggtgtttac ccgggaaacc gggatgacgc ccagccagtg gcgtcatctc aattcgcaga
    15421 aagattaatt cgccatgccc atgccgacga tgttggcggc gacaataatc accacacaac
    15481 cgaggctcaa caccgttacc ggacggcgac ctgcattgtt ccactctttc agcaccaacc
    15541 cgacgatacc gccgcacaat acatagaagc tcatatgcag catccaactg atgtagtcat
    15601 actgcgccgg aatgcgggcg tggccccagg catagaaaaa gaattgcaga taccacatca
    15661 acccgcccag tgctgagagt aacacattgt gaatgatcag cggttttgcc agcgagaagt
    15721 cggcttttag cgacaaatcc ttcacttttg ccagacgaat gaagcagaaa ccgaggttaa
    15781 tgatcgcgcc gccgcccatg atgacaacat agcttggcag agcgacatac agtggatcga
    15841 cgcccagtgc ggcagcggct tcatgcatcg gttttgcggc gttcatcgca aaggacattc
    15901 cggcagagaa aatgccgcac atcaccgcca gcaccagccc ttttttcagg ttgaactctt
    15961 cggctttaat gcccatcttg cgctctttca actgcccggc gcgagtcaca atccctacac
    16021 caatcagcgc caccagaacg ccgagcaatg tcatgcgtcc accttcggta ttgatcaaca
    16081 catcgaaatt gccgttgata attggcgtca tcagcgtacc aacaatcaac gtgataccaa
    16141 tggcgatacc aatccccatc gacatgccga gataacgcat ggtcaggccg tagttgatat
    16201 taccaatccc ccacattgcg ccgaacagaa aaacaggcag cagcgtagag agactaaacg
    16261 agctgtaata cgcccagaaa ttcggtagta acagggcgct gatggcccac ggcagaataa
    16321 tccacgaaac aatcccacca actgaccaca tggtttccca tgaccatttt tttacttttt
    16381 tgaacggagc gtaaaaacag gctgcactgg ccgcgccgat caaatgccaa aatatcccca
    16441 tcgtaatcgc gttactcatc ttcttatcct catcattttt cgtcgcgtca catctccgac
    16501 gagatgagtg taaaaatcgt gctgtcgatt aacctttcgc ctgttgccgc cgttgtcgat
    16561 ttactggcaa tcacggcatt aagtgggtga tttgcttcac atctcgggca ttttcctgca
    16621 aaaccatacc cttacgaaaa gtacggcatt gataatcatt ttcaatatca tttaattaac
    16681 tataatgaac caactgctta cgcggcatta acaatcggcc gcccgacaac actggagatg
    16741 aatatgagtt ataccctgcc atccctgccg tatgcttacg atgccctgga accgcacttc
    16801 gataagcaga ccatggaaat ccaccacacc aaacaccatc agacctacgt caacaacgcc
    16861 aacgcggcgc tggaaagcct gccagaattt gccaacctgc cggttgaaga gctgatcacc
    16921 aaactggacc agctgccagc agacaagaaa accgtactgc gcaacaacgc tggcggtcac
    16981 gctaaccaca gcctgttctg gaaaggtctg aaaaaaggca ccaccctgca gggtgacctg
    17041 aaagcggcta tcgaacgtga cttcggctcc gttgataact tcaaagcaga atttgagaaa
    17101 gcggcagctt cccgctttgg ttccggctgg gcatggctgg tgctgaaagg cgataaactg
    17161 gcggtggttt ctactgctaa ccaggattcc ccgctgatgg gtgaagctat ttctggcgct
    17221 tccggcttcc cgattctggg cctggatgtg tgggaacatg cttactacct gaaattccag
    17281 aaccgccgtc ctgactacat caaagagttc tggaacgtgg tgaactggga cgaagcagcg
    17341 gcacgtttcg cggcgaaaaa ataatcattt gccgcctgct gcaatgaggc gtttacgccg
    17401 catatcagct taaaaaatga accatcgc
//
LOCUS       DAZKJR010000107        17355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKJR010000107 DAZKJR010000000
VERSION     DAZKJR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.108"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..556
                     /locus_tag="ACHOWS_003632"
     CDS             38..556
                     /locus_tag="ACHOWS_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFQ0049154.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            572..1111
                     /locus_tag="ACHOWS_003633"
     CDS             572..1111
                     /locus_tag="ACHOWS_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFQ0049155.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1204..2781)
                     /gene="guaA"
                     /locus_tag="ACHOWS_003634"
     CDS             complement(1204..2781)
                     /gene="guaA"
                     /locus_tag="ACHOWS_003634"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0049156.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2850..4316)
                     /gene="guaB"
                     /locus_tag="ACHOWS_003635"
     CDS             complement(2850..4316)
                     /gene="guaB"
                     /locus_tag="ACHOWS_003635"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0049157.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4478..5854
                     /gene="xseA"
                     /locus_tag="ACHOWS_003636"
     CDS             4478..5854
                     /gene="xseA"
                     /locus_tag="ACHOWS_003636"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0049158.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDNAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5923..13974)
                     /locus_tag="ACHOWS_003637"
     CDS             complement(5923..13974)
                     /locus_tag="ACHOWS_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFQ0049159.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLIAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQTSETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHYAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAK
                     MRSDYTASDEKDVIFTVITSPDSDKARMWGHMLGIIEANNIFKRPRLADETDNELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGGGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14095..15072)
                     /locus_tag="ACHOWS_003638"
     CDS             complement(14095..15072)
                     /locus_tag="ACHOWS_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFQ0049160.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15127..17304)
                     /gene="sinH"
                     /locus_tag="ACHOWS_003639"
     CDS             complement(15127..17304)
                     /gene="sinH"
                     /locus_tag="ACHOWS_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFQ0049161.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYIINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPRLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4082 a         4611 c         4308 g         4354 t
ORIGIN      
        1 aatgtcttat ttttcgtaat gtgtataaca aggaatagtg atgaaattta aaaaatgtct
       61 tctgcctgtg gcaatgttag cgtcattcac tctggcagga tgccagtcaa atgctgatga
      121 tcatgccgcc gatgtttatc aaaccgatca actgaatacc aaacaagaaa ctaaaaccgt
      181 taatattatt tccattcttc ccgcaaaagt tgccgtagac aacgcccaaa ataaacggaa
      241 cgcacaagcc ttcggcgcgc ttattggcgc tgtcgctggc ggtgttatcg gccacaacgt
      301 cgggtctggc agcaattccg gaacgacggc aggtgcagtt ggcggcggag ctgtaggcgc
      361 ggcagcgggt tctatggtga atgataaaac cttagtggaa ggtgtttctt taacatataa
      421 ggaaggcacc aaagtgtata cctccaccca ggtgggtaaa gagtgccagt ttacgacagg
      481 tttagccgtt gttattacca cgacgtataa cgaaacacgt attcagccaa ataccaaatg
      541 tcctgaaaag agctaataat caggaggagt catgaagaaa gtttttcttt gcgccatctt
      601 agcctcctta agctatccgg ctatcgcctc atcattgcag gatcaactct cggctgtcgc
      661 agaagcggaa cagcaaggta aaaatgaaga gcaaaggcag catgacgaat gggtcgcgga
      721 gcgcaacagg gaaatccagc aagagaagca acgtcgcgca aacgcccagg ccgcggctaa
      781 taaaagagcg gcaacggcag cggcgaataa gaaagctcgt caggataaac tggacgccga
      841 agccactgcg gacaaaaaac gcgatcaaag ttatgaagat gagctacgca gcttagagat
      901 tcagaaacaa aaactggcgc tggcgaaaga agaagcccgc gttaagcgag aaaacgaatt
      961 tatcgatcag gaactgaagc acaaagctgc gcaaaccgat gtggtgcaat ctgaagctga
     1021 cgcaaacagg aatatgactg aaggcggtcg cgatctgatg aaaagcgtgg gcaaagcaga
     1081 agagaataaa tcagatagct ggtttaacta aacgatgtta gtaacttcaa acctataatt
     1141 ctttaggata aaaaaccctc tgtagtaaca gagggttttg ttcattcata gtgcagggtc
     1201 aaatcattcc cactcaatag ttgctggtgg cttgccgctg atgtcataca ccacgcggga
     1261 aataccattc acttcattga taatgcggtt ggaaacgcga ccgaggaaat cgtacggcag
     1321 atgtgcccag tgtgcggtca taaagtcgat ggtttcgaca gcacgcagag agacaaccca
     1381 gtcatactta cgaccatcgc ccattacgcc aacggaacgt actggcagga agacggtgaa
     1441 tgcctggctg actttgtcgt acaggtccgc tttacgcagt tcttcaatga agatggcgtc
     1501 agcacggcgc agcaggtcac aatactcttt cttcacttct cccagaacac gaacgccaag
     1561 gcctggtccc gggaacgggt gacggtacaa catgtcatac ggtaggccca gctccagacc
     1621 aatcttacgc acttcgtctt tgaacagctc tttcagcggt tcaaccaagc ccatcttcat
     1681 ctctttcggc aggccaccca cgttgtggtg agatttgatg acgtgtgctt taccggttgc
     1741 agaagcagca gattcgataa cgtcagggta gatagtgccc tgcgccagcc acttcacgtc
     1801 ttccagtttc agcgcttctt catcgaatac ttcaacgaaa acgcgcccga tgattttacg
     1861 ttttgcttcc ggatcatttt cgccagccag cgctgacaag aagcgatctt ctgccggtac
     1921 gtgaacaata ttcagaccaa agtgatcgcc aaacatatcc agaacctgct ctgcttcgtt
     1981 gagacgcagc aggccgttgt cgacgaatac gcaagtcagg tttttaccga tagcgcggtg
     2041 caacagcatt gcggttacgg aggaatccac accaccagag aggccgagga tgactttatc
     2101 gtcgcctacc tgctcgcgga tgcgagctac agcatcgtcg ataattttcg ctggcgtcca
     2161 cagggcttca cactggcaga tatcacgcac aaaacgctcc agcatgcgca taccctggcg
     2221 ggtgtgagtc acttccgggt ggaactgtac gccatagaag cgtttttctt cgttagccat
     2281 aatggcaaac gggcagcttt cggtgctggc tacggtgacg aagtcggacg ggatagcggt
     2341 aactttatcg ccgtggctca tccagacatc gagcagcggt ttaccgtctg cggtcagagc
     2401 atcttcaata ccgcgaacca gtgcgctgtc gtttacgact tcaacctgcg cgtagccaaa
     2461 ttcacgttcg ttagaggctt caacgtgacc gcccaactgc attgccatgg tctgcatgcc
     2521 atagcaaacg ccgaataccg gtacgcctgc ttcaaaaaca tactgcggcg cacgcgggct
     2581 gttttcttcg gtggtgcttt ccgggccgcc ggaaagaata atgccgcttg ggttgaagtc
     2641 acgaatttgt gcttctgtca catcccacgc ccacagttcg cagtaaacac ccagttcacg
     2701 cacgcggcgc gcaaccagtt gagtgtactg ggaaccgaag tccagaatga ggatgcgatg
     2761 cttatgaatg ttttccgtca ttgacgctta ttccgaggca agtgaaacag ataatataaa
     2821 tcgcccgaca tgaagtcggg cgaagagaat caggagccca gacggtagtt cggggactct
     2881 ttagtaatgg tcacgtcgtg aacgtggctt tcctgaatgc ccgcaccgct gatacgtaca
     2941 aactccgctt tagtacgcag ttcgtcgata gtaccacagc cggtcagccc catacaggag
     3001 cgcaggccgc ccatctgctg gtgaatgatc tctttcaggc gacctttata ggccacgcga
     3061 ccttcgatac cttccggcac cagtttgtcg gcagcgttgt cgctctggaa gtaacggtca
     3121 gaagaacctt tggacatcgc gcccagggaa cccataccac ggtaagattt gtaagaacgg
     3181 ccctggtaga gttcgatttc acccggagat tcttcagtac ctgccagcat ggaacccacc
     3241 atcaccgcgc ttgcgcctgc ggcgatagct ttggcgatat cgcctgagaa acggataccg
     3301 ccgtcagcga taaccggaat gccagtacct tccagggctt ctactgcatc agcaacggcg
     3361 gtgatctgcg gaacaccgac accagtcacg atacgcgtcg tacagataga accagggccg
     3421 atacccactt taacggcact gcaaccggct tctgccagcg cgcgggcacc tgcagctgtt
     3481 gccacgttgc cgccgatgat ttgcaggtca ggatatttag cgcgagtttc acggatacgc
     3541 tgcagaacac cttcagagtg accgtgagag gagtcgatca gcagaacgtc aacgcctgcg
     3601 gcaaccagcg catcaacacg ctcttcgtta ccagcgccag caccaaccgc tgcaccaaca
     3661 cgcagacggc cttgctcgtc tttacaggcg ttcggtttac gttctgcttt ctggaaatct
     3721 ttcacggtga tcatgccgat caggtggaat tcgtcatcaa ccaccagcgc tttttcaacg
     3781 cgtttttcgt gcatttttgc cagcaccact tcacgggctt cgccttcacg cacggttacc
     3841 agacgctctt ttggcgtcat gtaaacgcta accggttgat tcagatcggt cacaaaacgc
     3901 acgtcacgac cggtgatgat gccgaccagt tcgttttctt cggtaacgac cgggtagccc
     3961 gcaaagccgt tacgctcggt cagttctttc acttcgcgca gcgtcgtggt tggcagaaca
     4021 gtctgcgggt cagtcaccac accagattcg tgttttttca cacggcgaac ttcttctgcc
     4081 tggcgttcaa tggacatgtt tttgtggata aagccgatac cgccttcctg agccagagca
     4141 atagccaggc gcgcttccgt tacggtatcc attgctgcgg aaagcatagg gatattcaga
     4201 cgaatagttt tcgtcagctg ggtgctgagg tcagcagtat tcggcagaac ggtagaatgt
     4261 gcaggaacga ggagaacgtc gtcaaacgtc agagcttctt tagcgatacg tagcatgggc
     4321 aatatctcga ccagagtggt taataaatat tgccgcggca ttatacagag cgtaaccgat
     4381 tgcatctacc cctttttgca aaaaatgctt gctatccccg gagggcgggt tactatcgac
     4441 tgaataacct gctgatttag aatttgatct cgctcacatg ttaccttctc aatcccctgc
     4501 aatttttacc gttagccgcc tgaatcaaac ggttcgtctg ctgcttgagc atgagatggg
     4561 gcaggtgtgg atcagcggcg aaatctctaa tttcacacaa ccggcttccg gtcactggta
     4621 ctttacgctt aaagacgata acgcccaggt gcgctgcgcg atgttccgca acagcaatcg
     4681 ccgggtcacc ttccgcccgc agcatgggca acaggtttta gttcgcgcca acattacgct
     4741 gtatgaaccg cgcggcgatt atcaaattat tgtcgaaagt atgcagcccg ccggtgaagg
     4801 tttactgcaa cagaagtacg aacagcttaa agcgaagttg caggctgaag gtttgttcga
     4861 tcagcaactt aaaaaaccac tcccctcccc tgcgcactgc gttggggtga ttacctcaaa
     4921 aacaggtgcc gcactgcacg atattttgca tgtgttgaaa cgccgcgatc cgtctctgcc
     4981 agtgataatc tatcccactg ccgttcaggg cgatgacgca ccagggcaaa tcgttcgcgc
     5041 cattgagctg gcgaatcttc gcaacgagtg cgacgtatta atcgtcgggc gtggcggtgg
     5101 ctcgctggaa gatttatgga gttttaatga tgagcgtgtg gcgcgggcga tttttgccag
     5161 tcgcattccg atagtcagcg ccgtcgggca tgaaaccgat gtgacaattg ctgactttgt
     5221 tgctgatcta cgtgcgccaa cgccgtcagc cgccgctgaa gtggtgagcc gaaatcagca
     5281 agaattgcta cgtcaggtgc aatcgaccca tcaacggctg gaaatggcga tggattatta
     5341 tctcgccaac cgcacgcgtc gttttacgca gattcatcat cgcttgcagc aacagcatcc
     5401 gcagctccgg ctggcacgtc agcaaactat gctcgaacgt ctgcaaaagc ggatgagctt
     5461 tgcgctggaa aatcagctta agcgtgccgg gcaacagcag cagcgattaa cacagaggct
     5521 aaaccagcaa aatccgcagc cgcgtattca ccgcgcacag acgcgaattc aacaactgga
     5581 atatcgttta gcagaaaccc tgcgcgcaca gcttagcgcc acgcgtgaac gtttcggtaa
     5641 tgcagtaaca cacctcgaag ccgtaagccc actgtcaacg ctggcgcgcg gttatagcgt
     5701 caccagcgcc gctgatggcg cggtgttaaa acaggttaag caagtgaaag tgggtgagac
     5761 actgaccaca cgactgggag atggtgtggt gattagtgaa gtcagcgccg taacaaaaac
     5821 ccgcaaatca cgtaaaaaaa cgtctaatcc gtagaccgga taagaggcgc cagcatcgca
     5881 tccggcaata actcccaggc cacgccagat ccgccatgct ggttactgga agcaggtcac
     5941 cagacccggt ttatttatta gcgtgatact acaatcctgt gctccggtac cttcgtccat
     6001 cccacattgt tgagaggaca aataaggaaa actgggtgaa gacggccatc ccagtgaatt
     6061 aaaaacagta tgcaacaact ctaattcatt gtatgtcggt gcattttcta tgctgccaca
     6121 ctgccttgcg atatcccatt tactggcatc aatgatatgt gcagaagccc agttttcgta
     6181 gttaataatc ggtgaggata cgggattagc cccggacggc aattctgtcg ttaacaacgg
     6241 tcgatgtagc gatttaccat tcaccactac cgtgtctgcc atatgtcccc aaaagtgcgc
     6301 cgaggaaaca tttgggctgg ttggtaccgt aaaaatagcg tccagcgtgt ctgtcgcgga
     6361 agcgtaatta gccagagtaa ccgtcagtgg cgttttataa ccaggggtat tatcctggcg
     6421 gagcgtaaaa cttgccgtcc catccgcgcc tgtcatgcca ctaatggtat taccgctttc
     6481 actcagcgta acagccgaac cagataacgg ctgaacgatg agatcgtcca tcgccaccac
     6541 atcaccatac aacgttgccc cagcgttacg cggcgatgcc tcaccgcgtt tcaaagtaaa
     6601 ttccacattc ggcaccgtat tcccggcgct atctttcacc gtcacggtaa cgggcatggc
     6661 ttcgcctttt ttcactttcg ccgcctgagt ggctgcatca aacgctgttg aggtaagtgt
     6721 gacgctggaa accgttgaac gcgcgttagc caggcaaggc tgaaatgatt ttgttgaacc
     6781 cgtatacagc gtgccatttc tcaaatcaac cgactggtct ttaccatcgg cggatttatc
     6841 ctctgcccac caagtgtaat acgcaggcca gccagtttca gtgacaaacg tgttattcgg
     6901 ataacgcgca gacaacgaac ttaaatcatt gaatagtggc tgccttgcta cttcacaagg
     6961 gctcacactc gcatctgatt tctgtgttgg ggtaaagtac ggccagtctt cattattgtt
     7021 attgatcttg ccgacgccag aagtcgatgt cagttcactc cacaatttcg ggcgttcaaa
     7081 taccgcacca ttcactgccg tcaaggttgc tggcatatgc ccccagtagt tagccttcgc
     7141 agaatccggg ctggtcacta ccgtgaatat caggtcctga ttagccgtaa cctgcggatt
     7201 tcgcgccaat gaagctgtaa acggcgttac cagaccgacg gtgttatttt gggtaacgtt
     7261 gaacgttatc gtgccatcac tgccggtgaa gccgctccat gccgacgtat tctggttgaa
     7321 ggagatacgc gccgatgtcg gggtgacttc atctaaaatg agatcatcta cggtattgga
     7381 ggatacagag atccctgaac ggcttttcgc ctgcacgcgc ttgagcgtaa caccttcgcc
     7441 gccaacagga ttaccattgg catctttgat cgtcaccacg atgggaagca tctcaccttt
     7501 tttcacagat gacgtggggg taccaccatt gctggcagtt cggccctcat cataggcagt
     7561 cgatgacagc gtaaggcttg ctggtgccga atgtggctgc gtcagacaga gcattaaagc
     7621 gtatccatta atagtcgtcg tcgattgtcc cgttttcaaa ttaatgacgc cgtaatacca
     7681 cgtcaacgcg tcagtggtgc tcatttcata agtccaccaa tttccacttc cggcagttat
     7741 aggcaacccc aaatctgtca gtattgtccc gcctggatga tcattatata gcccctgtaa
     7801 ctcattgaga cgcggctgat actcaaccgg gcaatccggc gtaactttat tggtataaaa
     7861 aaggtatgac cacgactcat tattattact taccgaggac cccaaggtcg cttctgctgc
     7921 cagtagcggt cgtttaaagg tcacccccgc cctactggta aacgtgtcgg gcatatgacc
     7981 ccagtatttt gctttcggtg agttcggact ggttatcaca gtgaaaataa catccatcgt
     8041 ggcacttttt gtggtgtcgc gcatcaatgt tgcagtgagc ggagttgcca gcccataagt
     8101 ctcgttttgc gtcacatcaa aagtcgcttt accatcactt cctgtcgtca gaagtgcctg
     8161 tgccccatta ttatagagtg acatgtttac tccggcaggg gtgaccggca cgatagtaag
     8221 atcatcatac gtagaatcta gcctgttttt tgcacgatcc aacgcagtcc cgcgtatgag
     8281 attaaagttt gcgcctggca acagatttcc tgcgctatcc cgcacaatca ccgtcatccg
     8341 gatcgccgta cctttcgccg ccgttgcact agggcgacca tcgttgctgg ccgttttctc
     8401 cgcatccagt gccgtggagg tcatctctat agaagctgcc actgcatgag ggtttaccag
     8461 acaggacaca aatgccacgg tgtttgataa tgccctggag cttgatccat tcgataaatt
     8521 aatagactga tcgtaccaga cgttcgcgta ggcaatttta tcgtaagccc accaggtgtt
     8581 agtcagcgga aggcctaaat cggtccctat cgcaccatta ggatgttcac tgtataactc
     8641 ttgaaattcg cttaataacg gttgataagg ttcgccgcag tctgatttgc tgacatcatt
     8701 ttttgctgcc gtattcaaag aaagccatga ttcattatta gccatggttg actgccccgc
     8761 tggatgctcg gcagtaagca atggccgctt aaaggcaatt cccgaccggg tagtaaacgt
     8821 ttcaggcata tgcccccagt aacttgccag tggcgtatcc gggctggtta tgacggtaaa
     8881 catgaccggt aatttgttgg attctatcgt cggctgatac aactgggcgt acatattggt
     8941 taccaggccc atagaatcat cttgttttac cgtaaaggag gtagtcccat ccgcgccagt
     9001 caccccataa agcaggcttg aagaactatt catcctcgca gaagcgcctg ccgcgttaat
     9061 taccgtaatc gacttattta tcatctcggc attttgacgg ttcttcgagc cttcacgtct
     9121 gagaataaag gggacattgg gcacgctatt tccctgcgca tttttagtca ccacgcgaat
     9181 caccgcgtct tcgccttttt tcactttcaa cgcactcaag gtgtcgtcaa ctttgacaaa
     9241 ttgcgccgta tcattcgcct caacgatgat ctgactggca acggcgacag gcgtggtccg
     9301 gcagctcagt aatgcaccac cagatgaggg catctgcgtt tcatctcctg tcttcagcga
     9361 aacgacatca tacgtaaagg tttgagtgaa cgtatctgca acggtattcg agatatagaa
     9421 cctcgacact ggccagccaa tagcatcatg taatgcgcta ctcccctggt gcgcataaat
     9481 ggccctaagt tcattgtcag ttggaacata ctccgcgcca caaccattta tcgaggtatc
     9541 gttagcctgg cccgacggga acgtagacca ggcctccccc tcttccacca gggtaccgat
     9601 actggtggta gcactattct cgtccctcag taaaggcctt cgataaataa gaccgtcatc
     9661 gcctgtcgcc gtttcagcca tatgccccca gaatttggct tttggcgaat ccgggctggt
     9721 aagcaccgta aagacaacgg gaagcgtcga tttagtgctg ctggaggaat ccaacgctgc
     9781 cgtgagttct gtttttagcc cggtagtctg gtcttgcttc aatgtgaaag tggtgatgcc
     9841 atcagccccc gtcacgccat aaataaccag ggtcgttgaa agaaaatcat cttgagtatt
     9901 tccccaggca tccgttaccg ccagcgaagc aatcgacgtc gtagttgaca cattggccct
     9961 gttcacgctg gtgtttcgtt tgagggtaaa tgcggtattt cccacagggt taccctgcgc
    10021 atctttagtg gtcactcgta atacggcttc ttcgcctttt ttcgctttga caacattatt
    10081 cgtactgtca aattttgtgg ggtctgcagc ttcaagaaca atttggcttg ccttaacaat
    10141 cggcgtcgtc tggcaggtaa gcagttcttt tgtggcagat ggaattatcg aactggtgcc
    10201 tgaactgaga ttgacggcct tgaagtcacg ctgttccagg ctactactac ctgtcgtgct
    10261 ggaaaggtaa cttgaggcga cgggccagcc ctgaaccgtt ttcatcgcat tgccgcggtt
    10321 ggcttcatac aatgactcca gcccggcctg ggagggaaca tattcactac cacaaccatt
    10381 agaggatgtg cttatagcct gggtttgggt aaattgcgcc cagtcttcat tctcttctgc
    10441 aatcgctgtt cgacccgacg tactggataa ctctttcaac aataatgggc gtttaaagac
    10501 cgttccatct gcagccgtca ctgtttcagg catatgcccc cacattttcg ccttatcgct
    10561 gtccgggctg gtgacaacgg taaaaatggt atcgagggtc gcttttttcg ttgtgtctga
    10621 atagagcgcg gcggtcagcg acgtttttgt gccatgggta tccgggcggg taatagtgag
    10681 aattttagtg ccatcgctgc cggtcatcgc gctgtatgct gcggcggtat cattgagcga
    10741 agtttcatcc gccaggccac tattgatcac aaccggcgca accagcgcat caccgccacc
    10801 ggcaatgtgt ttttcgtttg atcgggtata accatcgcca cgcttcagcg taaaaggcat
    10861 atcacccaat ggattgccct gagcatcttt taccgtcacc ttcacctgta gcgtttcgcc
    10921 ttttttcagc ttcgccgcat ttgctgccgc gttccactgc gccgggtcca ccacttccag
    10981 cgtaatgctg ctggcaggat tgttagccgt cgtcaggcaa ctgacataaa ccgccgtgct
    11041 accgccaacg gtccgcgcgc catcgttgag ggctatggtg tagtaatgcg gcacctgatc
    11101 cgcgggcgaa ctgctccagt agggctgacg ctgcgttggc caaccgtgta ccgtttgaac
    11161 ggcgttaccg ctgttagcat catagagcgc gttaagctgg gaacggcgtg ggagcatatt
    11221 ggcaccacag ccacctttac tggtgctgga agtgttggcc tggctaacgc gcgaccacac
    11281 ttcgttattt tccgttaatg tgccgtccgg gctggacact tccgcagcca gccggggtcg
    11341 ggcaaaggtt aatgcatcga cggtaatcgt ctcatccata tgaccccaca tctgcgcgcc
    11401 aaccgcatcc ggactggtga gcgtagtgaa aatcacattg taattaacag tattgggaat
    11461 ataggagttt acaggcgcaa tattcagcgg cgtcgctaag cccacacctt gcggttgctt
    11521 aatttccaca gtagcaaaac cctgcgcatc cgtagtaccg gcatagacta aagacggttg
    11581 cgatgttccg tagagtgtgc cattcatctc gattgcgccg cgggttggat catcaaaacc
    11641 tgtagtctgc ccctgacgat ttttccccat atctttagtg atggtgaacg cggtatacgg
    11701 cacgggcgca ttattcaagg cattaaaggt gcgtaccgtc atggtaatcg tttcacccac
    11761 tttagcctgc gctctgctgc ttgtggaaac atcatgatcg gtggtcgcag caatctgggc
    11821 gaccatctca ttgcccgaac aagaaaggta gttttgctta aagccggaat aggtgtcgat
    11881 gttgcccgtg gccaggttca ccgacgcgtg cgacgtctct tccaccgcac taagatacgc
    11941 tttttgcaac gttggccagc catattcggt gccgatgaca ttggctggat gtgcggcaaa
    12001 caaactgtgc aatgagcttt ggctcggaat atgacctaaa ccacactcag cctgcatact
    12061 gctattctga tcaaacagcg cccagtcttc gttattttcc cgcactgctc ccagttcgtt
    12121 gtcggtttca tccgccagcc gtgggcgttt aaagatatta ttcgcttcaa taatgcctaa
    12181 catatgtccc cacatccgcg ctttgtcgga atcagggctg gtgatcaccg taaaaataac
    12241 gtctttctca tccgaggcgg tataatcact gcgcattttc gcggtgatgt gtgttttcac
    12301 ccccacgccg cctggctggg tcagcgtcag agaggcctct ccgttagcat cagtgatccc
    12361 ttcataggta tgcgcgtcaa ctttgcgaac gttactgcca ccatcaattt gtaccgggtg
    12421 cgtctcccac gcagcgtcag tttgattctt acggttaaca ccgtcatcca gatgcagcga
    12481 aaagtagtag taagccagcg gctgatcgtt gttcttgcta tcggtaatcg tcaggcgtaa
    12541 agaagcctct tcccccacct ttactttcat cgcttttatc gtcgaatcat aactgcccgg
    12601 cgtcagtacc agcttcggtt caacatcggg cgcttcttta tcgacacagc tcacaataaa
    12661 cgtcgtatcg tccggttttt gcgcttcgct gcggttcgaa acatccgccg catagtgctg
    12721 cccgctcatc cccgccagtg atgaccagta gtagttaccc tgcatcggcc aaccagccac
    12781 atcctgcact gacgttggca ccaccgtcgc cagcgcaccg aactgacgca ttccgggcaa
    12841 aatgtcgcaa tgtttatccg caccactcca ggtaaaggtg gaccaggttt cattgtgatc
    12901 gttaaccgta ccgtcttcat tatcgacttc cgccgccagt tttggtcgac taaaagtgta
    12961 atcacgggcc ttcagcgtat cgggcatatg accccacatt gtggcctgcg ggacatccgg
    13021 gctggtgagg acggtaaaga tcactgccgt ttcactggtc tgagcaatac ccgcgaggct
    13081 cgcgaccaac ggcgttttta caccagggcc gttggcctgg gtgaccgtaa ccgtggcaac
    13141 accattcgca tcggtagtac cacgatattc cgtcgcggtg gtggttaatt cggtcgtgcc
    13201 cagttttacc ggcgcagtat tgttcactac accctggcgg ttttcagcat ctttgcgttt
    13261 gataacaaaa ggaatattcc ccacaacatt accgacgcaa tctttggtgg tgaccgttaa
    13321 cgtgatactg ccgccaaccg tcgttttcgc cgcaccggag gattcgtcat agcccgcggc
    13381 gctgacggaa ataaggtcac cctgcgcggg cgcaatcttc agcgtcactt tctgatcggc
    13441 aagggtcaga acactttcat agggatagcc cgtcgaggta agtagatata acagcggcat
    13501 ggtctgctgg gggtctggct gcgcccaggc gatcagcgaa cgcccggcaa ggccctgtgc
    13561 gcaaaatgag cgatctgcga ctggctgtgg gtccagtaag gtgtccggcg tcgcggcatc
    13621 cgcccaaact aaagatactg tcggtggcgt ttggttttcc caacgtggtg tggcaatgat
    13681 atcgccttct ctgtcttgcg ggttggtgac gagcaattta gagacgctaa catccgcgct
    13741 gaattcactc ggtttagcgg taaacgctac ctcatgcgtc tttgcaggat ccagaaagac
    13801 actgccctgg tagacgggta tgctggcact gtcggcggtg ggcacggtac cgttaaattc
    13861 gccgaccgta tctgaagagt cttgccatgc agattctgcc catgaccacc caggcagcat
    13921 tgcgcttatc agcaatgcgc ataacacaag gattttattc agggtcaggg acatgacaga
    13981 aactccaccg gttgttaaac ggacgcttgt cgcatcaaca tattttgctt aatccgggat
    14041 taagcaaaat atgcggaaac agcattccct tgcgggaatg ctgctgagta cgcgttattt
    14101 gtgtttgtaa ataatgtgaa gtttgtaacc ctgtaccccg tcgccgtttg tcggaatggt
    14161 aagagcctct ttaccatcgc gcgcaggacc gttaaggttg gttctggctt cgtcgttggt
    14221 cgcagggatg gccagattgt tattatcgaa gcttgagtta acgataccac ctgaagtgcc
    14281 cagctgctgg ctgctgccag aaagtaccca acggaagtca taattggcgg taacgtcagc
    14341 atcggtaccc gcatcaaact tgccgttttt gttctcatcc gagtacagtt tggccacgta
    14401 ggttttgcca agttcgattg gcgtgctgcc gttgataagg ttgatactgg taccatcaac
    14461 aaagatggcg actttcaggt tctggttaac caccggccct ggcgcaacgt tatcgctgtc
    14521 gcttccgccg ccgctggcag tggaaatgtc ataaagatgc agcgcagtcc cgacgtttgg
    14581 cgtaccggtc tgcgtggtcg gcgtaatttc aataccgata taacgaccac ggtcgtcatc
    14641 ggtaagagtg taactggtca ccttcgttgc cagctctttc ggatcgcccc cctgagcgtc
    14701 cttgtagctc atccagcgga tagtatcgat agtctttgcg ttatcgccca atcccccaag
    14761 atccacgtcg ccttcggtat caccaatttc ccaacttacg gttaatttat cacccgagta
    14821 aagacgcttg tccgtttcac tgcctgtcgg gcgagcgcct tttagatcag aagtcaacgt
    14881 tacatggtcg gcatctgtcg tagcgcttcc cccttccgaa tagagccacg gaatggttcc
    14941 ctggaaaata ttggtttcgc cgctgtcgac ccaggcgctt tcttccgcca gcgccacagg
    15001 tgccacacaa taacctgcca gtgccagcgc caatgcgatc cgggtcagac cgcgtctttt
    15061 atcctgcttc attgatgctc tccttaatga accttcagcg actggccgac tggccttacg
    15121 ccaatcttat tttttggtat acagcacgcg taaaccgtat ccctgcaagc catctcccgc
    15181 ttgtgcgcca taggtttgcg ctgcttcgcg gttagtggcc ggaatcacaa tgtcctcatt
    15241 ggaagtgttc gcctgtgcac ccacttcacc attgatgctc ttgccggtaa attcccactt
    15301 gtagtcgtac tgcgccatct cttcgtcagt cagtttttcg acctgctgga agacaccgtc
    15361 tttattgctg tcacgccaca aaacaaatcg ataagtgttg ttgagcggca gcggttcttt
    15421 atctttacct atcaggttgc tgggtttagc cgcccccaca cgatagataa atgcatttag
    15481 cccaccaatt tctgccccga ggaaagtgac atccacatag ttactgtcgt cgtagggcgc
    15541 agcttttgct ttccagcgat aggtgccggg taaggtactg gagagggtga cagtaccctt
    15601 ttctttatct tgtttagcga taaaaacgca ggggttgtcg gtcgagcaag gcttgttggt
    15661 taacgtcact cgtttgtcct gcgcgtcgta aagccgcggc gtgaggttat cttccggcag
    15721 cgcatcgccg ttgcggtcgg tgttatcgcc aaagctgtcg gttacatcga gatccatcac
    15781 cacaccatca acgccatctg cctgggcgct ggcttcgtgg ctgtggttgt tctcttccgg
    15841 cacgttcgcg atgttattaa caatcaactc aattttacgg tcctgctgaa cggtgatttc
    15901 caccacacca gagttctgtc ggttgccctt ttcatcttcc agcgccagac gtactcgcca
    15961 ggtattgagt ttttcttgtt cggctttgtc agagctgtac tgccaggcgg gcagcaccag
    16021 gttccagtgg ttattgttac cctctgtcag ggtattctct ttgataaccg gcataaccag
    16081 cgtgcgggca gcattgtttt tcgcctgcca tgacgcagcg acaattttgt agtggtggtt
    16141 gataagcgcg ttaatggtcc acttacattt ttccagcccg accgccgctt caggcgtgtc
    16201 tttaatgaca cactcggggt gttcgttggt ggcatccgct tttaaccaca gggtgacatc
    16261 aagcggatct ttttctttgt attccagaac aataaagtta ttacgctcaa caaaatcatg
    16321 acgactgccc atcaacgagt gagccgcttt cactttatcg ctatccaatt gatcttttag
    16381 cggaacgccg aactgataat taagagtggc attaatgctg acatcgctgt tatctccggt
    16441 ccccgcttta tagtccgccc caacggttag taatggcacc ggttgataat tcagtccaag
    16501 tgttaccgcg tagggatctt tctccaaatt atccgtacca aaaagggcaa cttcatcgcc
    16561 gtaatattgc tcgaagacaa ctttgccccc cagttgcgga taagaaggta accaggcttc
    16621 ggcgcgaata tcccagccgc gcgcaggacg ctcctcataa aaatcgaaat cttcggagtc
    16681 tttccagtca gaaagcggat gataatagtt gccagagaat tttaaataat cagtccacgc
    16741 ttcgccgcct aaacctaaac ggcgatgtcc tcgggtaaaa tcataatcat aaaatatatt
    16801 tccgcccaat aaccattcat caaaattata tcttacccca agaccaatat tcccgattgt
    16861 acgatcttct tttcgctgcg cagaaaattg actgaaataa acagtggttt gattatcgta
    16921 ccagggaaca aaataatcga tggaactgcc atccagatcg ccgccctggc caatggagag
    16981 gttcgaacgg accttaccgt agggagagag agtgtcttca atataagatt gtgcggttga
    17041 ggtgatttga ctttgcagat agcttctggc ctgagattcc agcgcctcag gggtcaagtt
    17101 ttcaaaccct tgcgtggcgg agttgatgat gtaatcggct ccgcgctctt tcagcgattt
    17161 acccttgttg gtttgttcat cctgctgtgc tgcctggcta cctaaatcag gtaacgattc
    17221 gttttgctta acatttatct ctggcgccgc gaaagcagca ccagagataa atgttagaat
    17281 aatacagcgt ttccatcgca gcatgcactt tccctgtgac aatgatacag tcaaaaataa
    17341 gctttaatat tgtat
//
LOCUS       DAZKJR010000108        17340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKJR010000108 DAZKJR010000000
VERSION     DAZKJR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.109"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(277..1278)
                     /locus_tag="ACHOWS_003640"
     CDS             complement(277..1278)
                     /locus_tag="ACHOWS_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0049162.1"
                     /translation="MSSWLAMLQDATEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            1385..1681
                     /locus_tag="ACHOWS_003641"
     CDS             1385..1681
                     /locus_tag="ACHOWS_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0049163.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1804..2229)
                     /gene="arsC"
                     /locus_tag="ACHOWS_003642"
     CDS             complement(1804..2229)
                     /gene="arsC"
                     /locus_tag="ACHOWS_003642"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFQ0049164.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(2242..3531)
                     /gene="arsB"
                     /locus_tag="ACHOWS_003643"
     CDS             complement(2242..3531)
                     /gene="arsB"
                     /locus_tag="ACHOWS_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFQ0049165.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVILHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLFLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTSKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLTALALRLSFTL"
     gene            complement(3586..3939)
                     /gene="arsR"
                     /locus_tag="ACHOWS_003644"
     CDS             complement(3586..3939)
                     /gene="arsR"
                     /locus_tag="ACHOWS_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFQ0049166.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLSLLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQVIVRN
                     LARQNCSADSKNICS"
     gene            complement(4187..4402)
                     /locus_tag="ACHOWS_003645"
     CDS             complement(4187..4402)
                     /locus_tag="ACHOWS_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049167.1"
                     /translation="MMAISLQSEPPTQGWKVKNRRKVGFLHPDSDKAWYGSDPMKVYK
                     SSFTNMYSKLSLRQLKKSLTLAMLATG"
     gene            4681..4764
                     /gene="dinQ"
                     /locus_tag="ACHOWS_003646"
     CDS             4681..4764
                     /gene="dinQ"
                     /locus_tag="ACHOWS_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFQ0049168.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4818..6170)
                     /gene="gorA"
                     /locus_tag="ACHOWS_003647"
     CDS             complement(4818..6170)
                     /gene="gorA"
                     /locus_tag="ACHOWS_003647"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0049169.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6242..7084)
                     /gene="rlmJ"
                     /locus_tag="ACHOWS_003648"
     CDS             complement(6242..7084)
                     /gene="rlmJ"
                     /locus_tag="ACHOWS_003648"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFQ0049170.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLSSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            7287..9329
                     /gene="prlC"
                     /locus_tag="ACHOWS_003649"
     CDS             7287..9329
                     /gene="prlC"
                     /locus_tag="ACHOWS_003649"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFQ0049171.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILIRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            9337..10089
                     /gene="rsmJ"
                     /locus_tag="ACHOWS_003650"
     CDS             9337..10089
                     /gene="rsmJ"
                     /locus_tag="ACHOWS_003650"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFQ0049172.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10138..11607)
                     /gene="dtpB"
                     /locus_tag="ACHOWS_003651"
     CDS             complement(10138..11607)
                     /gene="dtpB"
                     /locus_tag="ACHOWS_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFQ0049173.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11922..12356)
                     /gene="uspA"
                     /locus_tag="ACHOWS_003652"
     CDS             complement(11922..12356)
                     /gene="uspA"
                     /locus_tag="ACHOWS_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFQ0049174.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12747..13082
                     /gene="uspB"
                     /locus_tag="ACHOWS_003653"
     CDS             12747..13082
                     /gene="uspB"
                     /locus_tag="ACHOWS_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFQ0049175.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13153..14652)
                     /gene="pitA"
                     /locus_tag="ACHOWS_003654"
     CDS             complement(13153..14652)
                     /gene="pitA"
                     /locus_tag="ACHOWS_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFQ0049176.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14884..16077
                     /gene="yhiN"
                     /locus_tag="ACHOWS_003655"
     CDS             14884..16077
                     /gene="yhiN"
                     /locus_tag="ACHOWS_003655"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0049177.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(16164..17216)
                     /gene="yhiM"
                     /locus_tag="ACHOWS_003656"
     CDS             complement(16164..17216)
                     /gene="yhiM"
                     /locus_tag="ACHOWS_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFQ0049178.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4540 a         4373 c         4443 g         3984 t
ORIGIN      
        1 aaagtgtatt taaaaatgtg ttgtattggc gactgaaatc tgccctttaa aaggggggat
       61 agcactgaaa caaagagaca ttgcttctcc gataaaatgt cagagaagca tgattgcgtg
      121 gtgattgtcg attgataact tactgacgga tccgtcatca ttctcaaagc attagttaag
      181 ttatttgtga tttttaagga ttatcctatc tatgattttc tactcagaag gataaggcac
      241 tcacaatgct ccttatagtt tactgctggc atctatttaa aatagcattt gagtcaaata
      301 gcccatcaag atagccatac ctaatataac cgtcaggaat gcaactatca tcggtattct
      361 gaacattgat ttaagtaata tcacttccgt caggcttgcg ccggcactgc cgatgattaa
      421 tgccattaat gctcccagac ccattccttt tgtcatcaga acagatgcca gagggataac
      481 cgcctctgcc cggatatata gcggaatacc gacaacggcg ctcaatggga tggcgaaggg
      541 attatctgcc cctgcatgag cggcaatcca ctctgaggga ataaagccat aaataaaaga
      601 gcctattaat acccctaaca aaagatatgg cagtacatct ttaaactgtt gcaacgcatc
      661 tttggtggct agttttatgg gattaatact taccacagct ttggtactgc aacagtttac
      721 tacaggtttc tcaatggctt tagccgctgg actacagcaa ctcactttta ttggcgtata
      781 tgtcgtcccc ggcgaagttt tggctggagc acaacaattt gctgatgagc ttttactggc
      841 aatgatatga cgttcaaatc ccagagaatc gaggataata ctggcaagta cggagacgcc
      901 ggcggcgata atcgcataca acaaggtaac tttccagcca aaggtcaccc acattaaccc
      961 gacgataatg ggattaagta atggggaaac aaataaaaaa gtgagggtcg gaccaaaccc
     1021 ggctttcgct gataacaatc cacgtaacat agggattgtc gagcaactac agaacggggt
     1081 gacggctccc aacagagcag ccaggagata gcctttccct tttcttgccc ccatcatctg
     1141 ctggatttta tggtctggca ccttttgtct tatcaggctc acaccggcac taatcactat
     1201 aaacagcaaa gaaagctcga cggcgagaaa cacaaacatc tctgtggcat cttgcagcat
     1261 cgcaagccaa ctactcatac ataacctcat tgaataaatc tatatttcca gaataatcga
     1321 aatgaaattc agatcaagat atttctggta ttatcgaaat atcaaccaga cagaggggga
     1381 tcatatgcag ttggaagaag tcgctaaagc gctgaaagag ctaggtcacc cgacccgttt
     1441 gttcatattt aaacatcttg tcaaagccgg ggaacaaggg ttgccggtcg gtgagttgca
     1501 aaagcagctt gggatacccg gctctacact cagtcaccat atttcagcac tggtatcggt
     1561 agggctggtc aaacaaaacc gtgagagtcg aacgctgatg tgtgtttcgc aatacgcaat
     1621 gctggaagcg ataattgaat tcttacgcga ggagtgctgc gtaaatagca aaattgcctg
     1681 atgcgctacg cgctacagat ttcatgccat gcacggtttt gtaggtcgga taagacgcgt
     1741 tagcgtcgca tccgacataa acaaagcgca ctttaccaac tatgggcagt ggatatcgcc
     1801 gctttatttc aagcgtttac ccgcttcatc aaccactttc tcgccatctt ccttggtgaa
     1861 cgcgccttgt tgcgcatctg gcagaatttc cagcacgact tctgaagggc ggcacaggcg
     1921 agttcccaac ggcgtcacca caatcgggcg attaatcaga atcgggtgct gaagcataaa
     1981 gtcgattaac cgatcgtcag taaatttatc ttctgcaagg cccagttcct catacggctc
     2041 gacgttttta cgcagcagcg cgcgtacgga aatccccata tcggcaatga gtttgaccag
     2101 ttcatcacgc gtcggtgggg tttccagata atagacaatg gtcggttctg tgccgctgtt
     2161 gcggatcatc tccagcgtgt tacgcgacgt gccgcaggcc gggttgtgat aaatggtaat
     2221 gttgctcata tcaatatctc attacaaagt gaaagagaga cgtagcgcca gcgcagtcag
     2281 cgtcacaaac agcacaggca gggtcatgat aatccctgta cggaagtaat atccccagct
     2341 gatagtcata ttcttctgcg aaagtacgtg cagccagagc agcgtcgcca ggctaccaat
     2401 cggggtaatt ttcggcccca aatcgcagcc aattacgttg gcataaacca tcgcttcttt
     2461 gataacgcca gatgccgtgc tgccatcaat ggacaacgcg ccaaccagta ccgtcggcat
     2521 attgttcata atagaagaga ggaaggcggt gaggaatccg gtgccgagcg tcgcggccca
     2581 caggccatta tccgccagca cgttgagtac gccagaaaga tattccgtta gtccggcatt
     2641 gcgcaggcca taaaccacca gatacatgcc gagcgagaag atgacaatct gccagggcgc
     2701 accgcgtagc accttactcg tattaatcgc atgaccgcgt ttagcgacga caaataatat
     2761 caacgcgccc actgccgcaa tggcgctgac cggaatgccg agcggttcca ggacgaaaaa
     2821 tcccaccagc agaaacagta aaacaaccca gccagttttg aaggtggcag gatctttaat
     2881 cgcttctgcg ggagatttca gcagcgccat atcgtagttc tgcggaatat ctttgcggaa
     2941 atagagatgc aaaatcacca gcgtggcaac aattgcggcg atatccaccg gcaccatcac
     3001 cgatgcgtat tcgcgaaagc cgaggccaaa gaaatctgcg gaaacgatat tcaccaggtt
     3061 ggagacaata agcggcaggc tggcggtatc ggcaatgaat ccggccgcca tcacgaacgc
     3121 cagcgtagtg cctttactga accctaaagc cagcaacatg gcgatgacaa tcggtgtcaa
     3181 aataagcgcc gcgccatcat tggcaaacag agctgcaacg gcagcaccga gcaggacaat
     3241 ccaggtgaac agcaagcgac cgcgtccatt gccccagcgt gagacgtgca gcgccgccca
     3301 ttcaaaaaag ccggactcat ccagcagcag gctgatgata atgacggcaa taaacgccgc
     3361 cgtcgcgttc cagacgatat tccacaccac cggaatatca cccggatgga ccacgcccgt
     3421 aactaacgcc agtactgcgc cgagcgttgc actccagccg atgcctaaac ctttcggctg
     3481 ccagataacc aatacaatgg tcaggacaaa gatagcgcct gccagtaaca taatgcctcc
     3541 cggataaaac acatgcgaaa atacatatgt gtttagctaa attttttaac tgcaaatgtt
     3601 cttactgtct gcggaacaat tttgtcgagc caggttgcgg acaatcacct gaaccttttc
     3661 ctgttcacat cgccaggcct catcaataat tttcgccgcc catgctggaa tatgcggtga
     3721 taagcggtaa tgaacccact taccttgctt gcgatccagc aatagcccac tttcacgcag
     3781 caatgacaga tggcgggata tcttgggctg cgactgatcg agggcagtgc agagatcgca
     3841 gacgcataac tctcccagtt cgctaagcag taaaacgatg cccagacggg tctcatcagc
     3901 aagaattttg aacagttgaa tgggtaacag aaatgacata ttgcgctcct gattgttgca
     3961 ggtagtgtct ctcttcgaag cggataagtc aaaaacatat atggtttaac gaatgtgtaa
     4021 gtgcagagga aggtaatagg tgtgaatttt gagttgggta ttcatttgaa aggaggtctg
     4081 aatcaggacg taaaaaaacc gactttgcgt cggtttttta ctttccagcc ctgagttggt
     4141 ggctctggct ggagtgagaa tgcccacatc taacggttca atactattag ccagtggcta
     4201 acattgcaag cgttagcgat ttcttcagct ggcggaggga cagtttgcta tacatgttgg
     4261 tgaaagagga tttatatact ttcatcgggt cgctgccgta ccaggccttg tccgaatccg
     4321 gatgtaaaaa accgactttg cgtcggtttt ttactttcca gccctgagtt ggtggctctg
     4381 actggagtga gatagccatc atctaacgca tcaatgctaa taccatactg aaactattgc
     4441 aaggacatgc tggttttata acccgcaagt actgtatgat tatccagtta gctctgagac
     4501 attttcactc tggcaatgcg cataaacgct ttcaaagtcc tggtcagaag tacgggtggt
     4561 gccgttaact gatgctctgg ccggagtgag aatgcccaca tctaacaatg gggcatgcgc
     4621 cgtgacaggc agtggatgag taagcggatg cattctcact ccatcgcatg gagaaaacgg
     4681 gtgattgata aagcaatcat cgttcttggg gcgttaattg cgctgctgga actgatccgc
     4741 tttctgcttc agcttctgaa ctgatagcgg aaacgtagct aagggctaag agcacactac
     4801 tcttagccct ttaacattta acgcattgtc acgaactctt ctgccgctgt cgggtgaatg
     4861 gcaaccgtgt tgtcgaagtc ttttttggtt gcgcccatct tcagcgccac cgcgaagccc
     4921 tgcaacattt catccatacc aaaaccaatg ccgtgaattc cgacaatctt ctcttccggt
     4981 ccaacgcata ccagtttcat gcggcacggc tggcggtgag tggtgacggc ggtatacatc
     5041 gcggtgaaag aggatttata cactttcacc tgatcgtcgc cgtactgctc gcgcgcctgc
     5101 ggttccgtta aaccaacagt cccaatcggc ggatggctga agaccacggt cggaatgttg
     5161 ctgtaatcca gatgctcatc cggcttgtta ttaaacaggc gttcagagag acggcgaccc
     5221 gctgcaactg ccaccggtgt cagttccact gcacccgtgt tatcgcccac cgcgtaaata
     5281 ccttcaacgt tggtattttg atatttatcg acgacgatat agcctttttc gttagtttta
     5341 acgccagcgg cttccaggtt gatgttgtca ttggcaggct cgcgaccaat cgcccaaatc
     5401 aggcaatcca ccgtttcact gcgcccatct tccagctcca gcgtcaggct accatcagca
     5461 tttttcacta ctgctttcgg gatggcgttg gtatgcaact gcgggccttc ggcgttcatc
     5521 acttcgacca gcgtttcaga gatcatcggg tcgaagctgc gcagcggcgc atgtttacgc
     5581 acaaacagat gcgttttcgc gccgaggccg ttaatcacgc ccgccagttc cacggcgatg
     5641 taacctgcgc caacaaccgc cacgcgctct ggcaaagcag gaagggcgaa gaagccatca
     5701 gaatcaatac cgtattccac gcctggaata tccgggtggc tcggacgacc gccggtggcg
     5761 atcagaatat gatcggccgt gatggtttcg ccgtttacct ccagcgtttt ggcatcaacg
     5821 aagcgggcaa agcctttgat tacatcaacg ttatttttac cgagcacgtt ttcataggaa
     5881 gtatgaatac ggtcgatata ggcggtacgg ctggcgatca acgtttccca gttgaattta
     5941 ttgatagtgg tatcgaaacc ataatccgga ccgtacatgt ggatcgcttc acggatttgc
     6001 gccgcgtgcc acatcacttt tttcggcacg cagccgacgt tcacgcaggt gccgcccagt
     6061 tctttggctt caatcagcgc gcatttctga ccgtacatag ccgcgcggtt gatggaggcg
     6121 ataccgccgc tgccgccgcc gatggcgatg taatcatagt gtttagtcat gacatattgt
     6181 ccttatcgtt gattaccgcg attgtagcgc gagacgtaat aggtgccagc gatggctgca
     6241 attactccgg cacgatccag cttgcggtgg cgtgcccagt gccagctgga accagtttac
     6301 tgtgcagcca cggcagcacg ttattcatct gctgttccag cttccacggc ggattaatca
     6361 caatcatgcc ggaagcggtc atgccacggc gatcgctgtc tggcagcacc gccagttcaa
     6421 tttgcagaat tttgcgaatg ccggtcgctt ccaggtcgtg gatcatgcgc ttaatttgct
     6481 ggcgcagcac cactggatac cacaatgcgt aagtgccagt ggcgaaacgt ttgtaacctt
     6541 ctgctatccc gctgaccacc gcctgatagt cggttttcat ttcatacggc gggtcgataa
     6601 ggattaaacc acggcgggaa accggcggca gcttggcctt aagctgctgg aaaccgtcgg
     6661 ctttttcgac gcgcgcacgg ctgtctttct gaaattcaga acgcagcaac gggtaatcgc
     6721 tcgggtgcag ctcggtcagt tgcaggctgt cctgttcacg cagtagctgg cgagcaatca
     6781 acggcgaacc ggggtaataa cgcaactgcc cgctacggtt gaagtgtttt accacattga
     6841 tgtacgcctc cagctccgca ggcaaatcgt cctgctgcca gatacgggcg ataccttcga
     6901 gatattcgcc ggtacgctcg gcatgttcgc tgcttaactg ataacgcccg gcccctgcgt
     6961 gggtgtcgag atagagaaac ggtttatctt tctctttcag cgactcgatg atcaggctct
     7021 gaacggtatg tttaaggacg tcggcgtggt tgccagcgtg aaagctgtgg cgataactga
     7081 gcatgggtaa aggtgttccg gtaagtaaaa atcggcccgt attcggggcg cacaaaagcc
     7141 tatcaggaca gtataccgaa aagaggccgc cgccgcgaaa gcgtaacgtt tctcattgaa
     7201 attcactaca cttaacccca tgctacacac attatgtaaa gcgcctgttg agcgcttcct
     7261 taacctcttt aaccaggact gcgctaatga cgaatccgtt actgactccc tttgaattgc
     7321 ctccgttttc taaaattctc ccggaacatg tcgttccagc cgtgactaag gcgctgaatg
     7381 actgccgcga aaacgtggag cgcgtagtag cgcaaggggc accgtacacc tgggaaaatc
     7441 tctgccagcc gttggcagaa gtagacgatg tgctggggcg tatcttctcc ccggtcagcc
     7501 acctgaactc ggtgaaaaat agcccggaac tgcgtgaagc gtacgaacaa accctgccgc
     7561 tgctgtcaga atacagcacc tgggtagggc aacatgaagg gctgtataaa gcgtatcgcg
     7621 acctgcgcga tggcgatcat tacgccacgc tgaacacggc gcagaaaaaa gcggttgata
     7681 acgcactgcg cgacttcgaa ctctctggca tcggtctgcc aaaagagaaa caacaacgct
     7741 acggtgagat tgccacccgt ctttccgagc tgggcaacca gtacagcaac aacgtcctcg
     7801 atgcgacaat gggctggacc aaactcgtta ccgacgaagc ggaactggca ggaatgccag
     7861 aaagcgcact ggctgcggca aaagcccagg ctgaagcgaa agagctggaa ggttatttgc
     7921 tgacgctgga tatcccaagc tacttgccgg taatgactta ctgcgacaac caggccttgc
     7981 gtgaagagat gtatcgtgct tacagcaccc gcgcttccga tcaaggcccg aacgccggta
     8041 agtgggacaa cagcaaggtg atggaagaga tcctcgcgct gcgtcacgaa ctggcgcaac
     8101 tgctgggttt tgaaaactac gcctttaaat cccttgccac taaaatggca gaaaatccgc
     8161 agcaggtgct tgatttctta accgatctgg caaaacgcgc gcgtccgcaa ggtgaaaaag
     8221 agctggcgca actgcgtgcc ttcgctaaag ccgaatttgg cgtcgatgag ttgcagccgt
     8281 gggatatcgc ttactacagc gaaaaacaaa aacagcatct ctacagcatc agtgacgaac
     8341 agctgcgtcc gtacttcccg gaaaacaaag cggttaacgg cttgtttgaa gtggtgaaac
     8401 gtatttacgg catcaccgct aaagagcgta aagatgttga tgtctggcat ccggatgtac
     8461 gtttcttcga actgtatgac gagaacaacg aactgcgcgg cagcttctac ctcgacctgt
     8521 atgcccgtga aaacaagcgc ggcggggcgt ggatggatga ctgcgtaggc cagatgcgta
     8581 aagccgacgg ttcgctgcaa aaaccggtcg cgtatctgac ctgtaacttc aaccgcccgg
     8641 taaatggtaa accggcactg tttacccatg acgaagtgat caccctgttc catgaattcg
     8701 gtcacggcct gcaccatatg ctgacccgca ttgaaactgc tggtgtatct ggtatcagcg
     8761 gggtgccgtg ggatgcggtc gaactgccga gtcagtttat ggaaaactgg tgctgggagc
     8821 cggaggcgct ggcgtttatc tccggtcact atgaaaccgg cgaaccgctg ccgaaggagc
     8881 tgctggataa aatgctggcg gctaagaact accaggcggc gctgtttatt ctgcgccaac
     8941 tggagttcgg cctgtttgat ttccgcctcc atgccgagtt ccgcccggat cagggagcga
     9001 aaatcctcga aactctggca gaaatcaaga aactggttgc cgtagtaccg tctccatcct
     9061 ggggccgttt cccgcacgct ttcagccata ttttcgccgg tggttatgcc gcaggttact
     9121 acagctacct gtgggccgac gtgctggcgg cagatgcctt ctcgcgtttt gaggaagagg
     9181 gcattttcaa ccgtgaaact gggcagtcgt tcctcgacaa cattctgatc cgtggcggtt
     9241 cagaagagcc gatggagctg ttcaaacgct tccgtggtcg tgaaccacag ctggatgcga
     9301 tgctggagca ttacggcatt aagggctgaa cattcagtga aaatctgctt aattgatgaa
     9361 acaggcgccg gagacggcgc cttatctgtt ctggcggccc gctgggggct ggagcacgat
     9421 gaagacaacc tgatggcgct ggtgttaaca ccggagcacc tggaactgcg caagcgtgat
     9481 gagccgaagc tgggcggcat ctttgttgat tttgttggcg gggcgatggc gcaccgacgc
     9541 aaattcggcg gtggtcgcgg agaggcggtg gcgaaagcgg tgggcattaa aggcgattat
     9601 ttgccggatg tggtggacgc caccgcaggg ctggggcgcg atgcctttgt gctggcttcg
     9661 gtcggctgcc gcgtgcggat gctggagcgt aatccggtgg ttgctgcatt gctcgacgac
     9721 ggcctggcgc gtggttatgc ggatgcggaa atcggcggct ggttgcagga gcggttgcag
     9781 ttaattcacg cttccagcct gacggcgctg accgatatta ctccgcgccc gcaggtggtc
     9841 tatctcgatc cgatgttccc gcataagcag aaaagcgcgt tggtgaaaaa agagatgcgt
     9901 gtctttcagt cgctggtggg gccggatctt gatgccgatg gattgctgga gcctgcacgc
     9961 ttgctggcga ccaaacgcgt ggtggtaaag cgcccggact acgcgccgcc gctggcgaat
    10021 gtcgccacgc caaacgcggt agtgaccaaa gggcatcgct ttgatattta tgccggaacg
    10081 ccggtgtaaa agtaaagcag ggtagcgtta tcgctaccct gctttctgca agaataatta
    10141 atggctttcg ggcgtcgcaa tcatgcgttt cagccacggc accatcagca gcatcaccac
    10201 cgcaacgccc agcgtaacca ggccaatctt accaaacacg ttggtataga cgggcaacgt
    10261 ctcaagcgga tcggtaatgt tgtctggtac tgcggtaaat gttgccacat agccgcccag
    10321 caagaacgcg gcagcctgcg tcaggaacca catcccgaga ataaagccca tcaaatgctg
    10381 cggcaccagg gcagcaatca tcgccagacc cagggcgcta ataaacaatt cacccaggct
    10441 ctggaacaag taaaccagca cgataaacca cggcgatgtc agtccttgtg catcagcaaa
    10501 ccacattccc gcagcggcgg ctgtaagaaa gcccagcgag cacataaaca tgccgagagt
    10561 aaatttcatc ggcatcgaga ggtctttgcc tttgttaccc agatgcgtgt aaatgcctgc
    10621 cagtattggg ctggcgagta ccacccagaa cgggttaagc gcctggaagc tgactgggtt
    10681 gatggaaaag ccgagaattt catgatgcac gttgttgata gcaaagaagt tcagcgatgt
    10741 tggcatctgg gcgtagagaa tgtaaaacac caccgcttcg agcatcagga caaaggcgac
    10801 aaacatttta ttgcgcccgg ttttatccag cttgaatgcc tgacgaaaga aaatgatggt
    10861 gacgacgatg gagagaacaa tcagcaccag attggcgact tctacgttgt gcatcagcca
    10921 tgcacagacg aagatcatca ccacgctgcc aagtaacacg tacaacagtt tgctgaagct
    10981 catcggccgg aagtcgggtt cagaaccaat gtctttcacc attccgcgac aggcgatgta
    11041 aaccaatagc gcgataatta atcccgcacc gcacaggttg taggtgactg aatagccgaa
    11101 tctatcagcg atcacagggg ccagcgataa cgctatcaac gagccgatgt tgatcgacat
    11161 atagaacagg gtaaatgcgc catcaagccg cggatctttc ggcggatagc acttcgaaag
    11221 caagctggct gggttagctt taaacaggcc gttaccgaca gcgatagtcc ccagggcgat
    11281 gaaaatcagg tcaggcttaa gtagcgacag gccggtcatg aagtagccaa tcgccagcac
    11341 aagtgctccg aggacaatgg tgcgtttggt ccccagcagg tggtcgccga catagccgcc
    11401 aatggaaatg aggccataga ccagcgcagc aaaagcacca aaagtgacaa aagcctgttc
    11461 ttgcgagaat ccaagctgct taacgaagaa aaccgccagt acgccctgca cgccgtagta
    11521 gccgaatcgc tcccataact cgacaaaaaa gatcatgaaa aatgggcgag gttgctgcag
    11581 catccccatg ggtgttgttg tattcatatt tattaacctt ccaataccat cctgaaaagt
    11641 atgaacgcgt aagggataac gcgcgaaaaa gtttacatct gtcagagatt cttaatattg
    11701 atgcggtgaa tagattaata cactattaca acagaaaata accagctgat cacactaatg
    11761 atggaaaata tgatggtgtt aaatgttgta ttggcagggg attttgattg cgtacgcaga
    11821 ccgtggccag ataaggtgtt tacgctgcaa ggtaacaagg cgcgccaaaa agcaaaacgc
    11881 ccgcatatgc gggcgttcag gaacacgtcg tagagggaag attattcttc ttcgtcgcgc
    11941 agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc
    12001 agtttgctcc agaagtcctg gtggtgacca caaaccacca aatccatatc gtatttcttg
    12061 attgcatcga ccagaacctg gcccaggtcg ccgctgccgc tcagggtttc agtgattggg
    12121 tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc
    12181 tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta gtttacatct
    12241 acgtggatca gagaaacttt cgcattgtag gggcgagcca tagagactgc tttctctacc
    12301 agaactttgc tttccgggga gagatcgacc gcgatgagaa tgtgtttata agccatagtg
    12361 ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg ccgttacgtc
    12421 acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag
    12481 accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt acccgttaga
    12541 taaacaaatt ttcgacatga atatattgat agtggttaac cttctggaaa aaaaacaacc
    12601 tgatctccta cactatctat agagccgctc ggatgttggt caccacaaaa gcggtttctc
    12661 caggacctgg tgttaactga ttggcagcgt atcgggagcg gtagccccgg ggtggaactc
    12721 cgcgggcagg tcgccgggga ggaggtatga taagcaccgt cgcattattt tgggctttat
    12781 gtgtcgtttg cattgttaac atggcgcgct atttctcatc actacgcgcg ttgttagtgg
    12841 tactgcgtaa ctgcgatcca ttgctctatc aatatgttga tggagggggc ttttttacct
    12901 cacatggcca acccaacaaa caggtgcgtc tcgtttggta tatctatgcc caacgttatc
    12961 gcgatcatca cgatgatgag tttattcgcc gctgtgagcg ggtgcgtcgg cagtttattc
    13021 tgaccagcgc attgtgtggt ctggtggtgg tcagtctgat tgcattgatg atttggcatt
    13081 aagcacttca gcaaaaataa cgcataaaaa aagcgggcca gtaaactgac ccgctcgttt
    13141 tggtgcgtgc gattacagga actgcaagga gagccagtac agcccgccgg aaagcagtac
    13201 cgcagccgga agggtaaaca cccaggccat cagaatactg gtcacggttt tacgctgtaa
    13261 gccgccgcca tctaccacca tcgtccccgc gacagaagag gagagtacgt gggtagtgga
    13321 aaccggcatc ccggtataac tcgccaggcc gatagacact gccgcagtca tctgggcaga
    13381 cattccctga gcgtaggtca tgcctttctt accgattttc tcaccgatag tcgttgccac
    13441 acgacgccag ccaatcatcg taccgatacc taacgccagc gcgaccgcca tgatgatcca
    13501 caccggtgca tactcgatgg tgctaagcat gtcagacttc agtttcttca acaggcgctg
    13561 atcgtcagca ctcacgccag gcatcttcac cactttgtcg atagtgtcag aaacgcacag
    13621 cataatgcgg cgcatctggc tacgttgatc aagcgacagc ttatcgtagc tttccacatc
    13681 ggtagtcaac atgcctttca ggcggttgag cgcgttaatg gtattcgacg gatggcaatg
    13741 gaactccgca ggttgcgttg cgccagcttc cggagccggt actaactgat cagcaccggt
    13801 cgcttgtttc agcagcgcag gatgctgctc aaagtaagct tcgacgttgt tgatggcatc
    13861 acgggtacgg gtgatttcgt agccagtggc attcatgttc accacgaagc ctgctggtgc
    13921 gacaccaatc aataccaaca taaccagacc aatgcctttc tgaccatcgt tcgcgccatg
    13981 cgaaaacgcc acgccgatag cggaaaggat cagcgcaata cgcgtccaga acggcggctt
    14041 tttcttgccg tctttctttt cacgctccgc tggggtcagg tggatacggg cgcgtttctt
    14101 ggtgccgctc cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca ccaggccgac
    14161 aataggggaa acgatcagag aaccgaaaat acttaatact ttcgggatat tgagtgcatc
    14221 caccactgac gtcccggtca tcaacgcatt ggttaaacca atcccgatga tcgcgccaat
    14281 cagcgtatga gagctggatg caggtaaacc aaagtaccag gtacccaggt tccagataat
    14341 cgccgccagc aacatagaga acaccatggc aaggccatga gacgatccca tattaagcag
    14401 cagatccgtc ggcagcatat gcacaatggc ataggcaaca ctcagaccac ccagcaaaac
    14461 acccaaaaag ttgaacaccg ccgccataac cacggcgagc tgagaacgca tcgcgcgggt
    14521 atagataacg gttgccacgg cgttggctgt gtcatggaaa ccattgatgg cttcgtagaa
    14581 cagcacaaaa gccagtgcaa gcaataataa cagcccggta tgcaaatcca ggccagcaaa
    14641 caaatgtagc ataggacgtt acgccatttt gaggacatga acgcggcgca ttatcagtga
    14701 ctttcgcggc gcgggcaaag tgaaatatag actttttttg atttgcctcc tgtatggatt
    14761 tcactcaaaa aataattatc ttatataatt caggtaaata cttcctttta gtaatattga
    14821 tgctggtgcg accactgagg aatctttaca attcacgccc gttttttctt agaggagcgc
    14881 aaagtggaaa ggtttgatgc cattattata ggcgctggtg cggcgggtat gttctgttct
    14941 gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa aaaaccaggg
    15001 cgcaaaatcc tcatgtctgg cggtgggcgc tgcaacttta ccaaccttta tgtcgaaccg
    15061 ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg ttttacccag
    15121 tgggatttca ttgatctggt caataaacac ggcatcacct ggcacgagaa aacgttaggg
    15181 caactcttct gcgatgactc cgcgcagcag attgtcgaca tgctggtgga tgagtgcgag
    15241 aagggcaatg tgaccttcag attgcgtagc gaagtgctga gtgtggcgaa ggatgaaaca
    15301 ggcttcacgc ttgaactgaa cggcatgact gtcggttgcg aaaagctggt catcgcgacc
    15361 ggtggactgt caatgccagg gctgggcgcg tcgccgtttg gttataagat tgccgaacaa
    15421 tttggcctca acgtgctgcc gacccgcgcg ggcctggtgc cattcactct gcataaaccg
    15481 ttgctcgaag agttacaggt gctggcgggc gtggcggtgc cttccgtgat taccgctgaa
    15541 aacggcatcg ttttccgtga gaacttactc ttcacccacc gcggcttatc tggaccggcg
    15601 gtgttgcaaa tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa tctgctaccg
    15661 gatgtggacc tcgaaacctt cctgaatgag cagcgtaatg cacatccgaa tcagagcctg
    15721 aaaaacacac tggcggttca tctaccgaag cggttggttg aacgtttaca acaactcggg
    15781 caaatcccgg atgtttcgct aaaacagctc aacgtgcgtg accaacaggc actgattagc
    15841 acattgaccg actggcgcgt acaacccaac ggcactgaag gctatcgcac tgccgaagtg
    15901 acgctcggcg gcgtggacac caacgaactc tcttcacgga cgatggaagc gcgcaaagtg
    15961 cctgggctgt acttcatcgg cgaagtgatg gacgtcaccg gctggctggg gggctataac
    16021 ttccagtggg cgtggtcgag tgcatgggct tgtgcgcagg atttggtggc agaataagaa
    16081 gcaaaatgtg ctctcgatac cagagggatt aacctgaata aaaccagata aggcgtccaa
    16141 cgccttatct gaagcctttt ttattatttt ttcgctgaac ccgcttctaa tatagaaacg
    16201 atggaaaaca gtgtaaaaca aactgcacct aagccaacca gaactcgcga aggaataaag
    16261 taagccatat cggtttgcgc aatttcagcc aggaacgcag caaggaacag acaaaacaga
    16321 caggtaaata ccggaatcat gggtattctg ttggctaacg aacaggtgcg tcgccatacc
    16381 agcgctaaca accacacttt ggagaaaata ctgtagcaga tcatccccag gcaaatgaga
    16441 ataatgccgg gggccaggcg ggcgcttgca tctgaactga ttaatacgta gataccaaaa
    16501 ataatggtaa gtgagccaag caaaataacc caatagcacc acagccaatg ttctttcact
    16561 gagaacgtat tccgcgtttg atggacaata gtggcgacaa ggccaataag acaagcgcaa
    16621 attgccgtta accccattag tacgtgacct gcaacataat gtggtgtgat atctgcacta
    16681 cgtaacagta tgactgacca aatgaaacca aagagagtaa gcagaacggg aacggcaatt
    16741 aagcaattac cgattgttga agaataggcc tgcaatggcg taccgtcact cttactgccc
    16801 gaggcgtttt tggggatcaa cagaaaatgt ccggaagatg ccgctacggt tgatacacaa
    16861 gcggcaatca tgccaacgcc gaaaataaca tgaccggcga caaattcatc tgccatcaca
    16921 ttattgcttg ccaacagtga ccatccccaa atcatagttg tagcagaacc ggcataacca
    16981 ataacgggaa ataacctgtt gtagaattta ttcatgccgt gagttaactg agatataata
    17041 ataaatgcgg tagtgaacaa tgccagacat atcgcagaaa gagaaatcag aacatgccct
    17101 gcaacgaaat attcactttg cccagaaccg gttaatacaa actcacctaa tgcgatgcat
    17161 attataccca tcagtaaagg aattaattta aacaaccatc cgatatatat attcatttta
    17221 aaccccatat atattttctg ctacacttcg aaaaacatat gattcgtctt ttcagttagt
    17281 taaatatgtc tataagatgc catcaattaa aaaaagtaac ttcactataa tcggatttag
//
LOCUS       DAZKJR010000109        17326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKJR010000109 DAZKJR010000000
VERSION     DAZKJR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.110"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(54..455)
                     /gene="crl"
                     /locus_tag="ACHOWS_003657"
     CDS             complement(54..455)
                     /gene="crl"
                     /locus_tag="ACHOWS_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFQ0049179.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(513..1757)
                     /gene="frsA"
                     /locus_tag="ACHOWS_003658"
     CDS             complement(513..1757)
                     /gene="frsA"
                     /locus_tag="ACHOWS_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFQ0049180.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(1849..2307)
                     /gene="gpt"
                     /locus_tag="ACHOWS_003659"
     CDS             complement(1849..2307)
                     /gene="gpt"
                     /locus_tag="ACHOWS_003659"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0049181.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2568..4025
                     /gene="pepD"
                     /locus_tag="ACHOWS_003660"
     CDS             2568..4025
                     /gene="pepD"
                     /locus_tag="ACHOWS_003660"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFQ0049182.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4082..4434)
                     /gene="prfH"
                     /locus_tag="ACHOWS_003661"
                     /pseudo
     CDS             complement(4082..4434)
                     /gene="prfH"
                     /locus_tag="ACHOWS_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(4430..4636)
                     /locus_tag="ACHOWS_003662"
                     /pseudo
     CDS             complement(4430..4636)
                     /locus_tag="ACHOWS_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(4954..5406)
                     /gene="yafP"
                     /locus_tag="ACHOWS_003663"
     CDS             complement(4954..5406)
                     /gene="yafP"
                     /locus_tag="ACHOWS_003663"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0049183.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFSMRYKPQH"
     gene            complement(5403..6458)
                     /gene="dinB"
                     /locus_tag="ACHOWS_003664"
     CDS             complement(5403..6458)
                     /gene="dinB"
                     /locus_tag="ACHOWS_003664"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0049184.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(6529..7314)
                     /gene="lafU"
                     /locus_tag="ACHOWS_003665"
     CDS             complement(6529..7314)
                     /gene="lafU"
                     /locus_tag="ACHOWS_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFQ0049185.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            7286..8998
                     /locus_tag="ACHOWS_003666"
                     /pseudo
     CDS             7286..8998
                     /locus_tag="ACHOWS_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(9216..9713)
                     /gene="rayT"
                     /locus_tag="ACHOWS_003667"
     CDS             complement(9216..9713)
                     /gene="rayT"
                     /locus_tag="ACHOWS_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFQ0049186.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKQ
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLQNMWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(9890..10639)
                     /gene="yafL"
                     /locus_tag="ACHOWS_003668"
     CDS             complement(9890..10639)
                     /gene="yafL"
                     /locus_tag="ACHOWS_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0049187.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            10951..11691
                     /gene="dpaA"
                     /locus_tag="ACHOWS_003669"
     CDS             10951..11691
                     /gene="dpaA"
                     /locus_tag="ACHOWS_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFQ0049188.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(11662..12429)
                     /gene="yafJ"
                     /locus_tag="ACHOWS_003670"
     CDS             complement(11662..12429)
                     /gene="yafJ"
                     /locus_tag="ACHOWS_003670"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFQ0049189.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(12545..13123)
                     /gene="lpcA"
                     /locus_tag="ACHOWS_003671"
     CDS             complement(12545..13123)
                     /gene="lpcA"
                     /locus_tag="ACHOWS_003671"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFQ0049190.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13363..15807
                     /gene="fadE"
                     /locus_tag="ACHOWS_003672"
     CDS             13363..15807
                     /gene="fadE"
                     /locus_tag="ACHOWS_003672"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFQ0049191.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(15850..16296)
                     /gene="ivy"
                     /locus_tag="ACHOWS_003673"
     CDS             complement(15850..16296)
                     /gene="ivy"
                     /locus_tag="ACHOWS_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFQ0049192.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            16477..17247
                     /gene="yafV"
                     /locus_tag="ACHOWS_003674"
     CDS             16477..17247
                     /gene="yafV"
                     /locus_tag="ACHOWS_003674"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFQ0049193.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
BASE COUNT         4136 a         4505 c         4527 g         4158 t
ORIGIN      
        1 acgtatcagt tttaatgaat attgccggat gtgatgcatc cggcacattt cattcacgcc
       61 gttaacttca ccggttcatc acgaaaatca tctgccggtt ccagcttcag attcagcgtc
      121 gccagcagct cacgcagctt ctcgtgaaat tcacgcaggg tgtgctccag tcgttcaacc
      181 acttcggcat cttttatctg aacactcgtc cagtcgcctg ctttatcgaa cagaccaaac
      241 tggtaactgt aggtaaaacg ggattcctgc gcttccagtt ccatccacca gccccagaac
      301 tcacgcactt ccggtgccgg tttcacgttg acgcatacag ccagacaatc gaaaaagaat
      361 cgattatctt cgcacttacc ttcacgaata tacgggccta gtgcggtaaa ttttttgatc
      421 aatctgctct tcgggtgtcc actcggtaac gtcattgcaa tctcctgttg tgatgcaact
      481 gttttaccaa attggtaaaa tttagcaaat ttttaacaca aacgtttttc gatccagtct
      541 gtgatttcct gaaggccttt gtcaaaattc cgatacaccg ggttaaatgg gatctctaat
      601 aatttaccgt cagcagatga cgacgtgatt aagcgtgagt cctcttccgg gctgaacgga
      661 tcgttcttcc agtagcctga taacattggc gttgggcagc gacgtcccag caatccttgc
      721 acttttaatg aatagcgatt caactccact cgcaacgctt catcggaagc atcatgcatc
      781 cctaaacgac tggccagaac gtcaagatac atttccggca cctgttgctg gcacttaaaa
      841 tcactcaaca gggtatgaac taccggacca agacaggcaa ccactttcag acgcggcgat
      901 tcaaggtatg ccagacgcac ggcaacgtta gcgccgaaac ggaaaccaaa ggccgcgacg
      961 cgagtgtgat ccacccacgg tacgttaggc agcgccttta aaacgtgctg atgcaacagg
     1021 ctggagtcct gggtgagctt ccattttgaa gaaaagccca ccgacggcat atcaatagtc
     1081 agcatagcaa tgccgcgcgg cgcaaaataa cgttcataca ggctgtaata gtccgtctgc
     1141 atcgcatcca gaccaccaca cattaatact gtcgggaacg ggccatcgcc tttcggcata
     1201 tgcaaaaagc cggtgatggg cgcaccgccg ggtacggtaa actccatctg ccgcatcgtg
     1261 cccggcagac gctgagcggc ctcttcatag gcgcggtttg acaaagcctg cgcttgctcg
     1321 gccaggtcat ctcctttcag atgaggatag gcagcaatgt tgtacaacgt agccgcatgc
     1381 agccagtatc gaccgctgag ttgcggatct tcttccgcac aggctttttg ttgccacacc
     1441 atcgcctggg tagcccactc ataaatccag ttgccgccgc ggtagccaat caccgtatcg
     1501 tataaatcat cgtcggtacg ttcggcatcg ctcatcacaa tacgcgcctg gacgtcgagg
     1561 atttcacggg ggtcaatgcc gcgccagatc cacatcagac ggttaatcat gcgataccag
     1621 tgagggatgg ttttaccatc aagggccgac tgcacaggca gttgtgcgcc gtgactaaag
     1681 cggcggacta gcgtcgaggt ttcaggatgt ttaaagcggg gtttgaacag ggtttcgctc
     1741 aggtttgcct gtgtcatgga tgcagcctcc agaatactta ctggaaacta ttgtaacccg
     1801 cctgaagtta aaaagaacaa cgcccggcag tgccaggcgt tgaaaagatt agcgaccgga
     1861 gattggcggc acgaatacga cgcccatatc ccacggctgt tcgatccagg tattttgcgg
     1921 gatatcaaca acataatcat caaccagcgg acgaccagcc ggttttgcga agatggtgac
     1981 aaagtgcgct tttggataca tttcacgaat cgcaaccgca gtaccaccgg tatctaccag
     2041 gtcatcaata acgatgaagc cttcgccatc gccttctgcg cgtttcagca ctttaagctc
     2101 gcgctggttg tcgtgatcgt agctggaaat acaaacggta tcgacatgac gaatacccag
     2161 ttcacgcgcc agtaacgcac ccggtaccag accgccacgg cttacggcaa taatgccttt
     2221 ccattgttca gaaggcatca gtcggcttgc gagtttacgt gcatggatct gcaacatgtc
     2281 ccaggtgacg atgtattttt cgctcatgtg aagtgtccca gcctgtttat ctacggctta
     2341 aaaagtgttc gaggggaaaa taggttgcgc gagattatag agatctggcg cactaaaaac
     2401 cagtatttca catgagtccg cgtcttttta cgcactgcct ctccctgacg cgggataaag
     2461 tggtattctc aaacatatct cgcaagcctg tcttgtgttg acaacatttt ctgctaaccc
     2521 tgtgacctgc aatactgttt tgcgggtgat cgacaaggag acttaacgtg tctgaactgt
     2581 ctcaattatc tccacagccg ctgtgggata tttttgccaa aatctgttct attcctcacc
     2641 cgtcctatca tgaagagcaa ctcgctgaat acattgttgg ttgggcaaaa gagaaaggtt
     2701 tccatgtcga acgcgatcag gtaggtaata tcctgattcg taaacctgct accgcaggta
     2761 tggaaaatcg taaaccggtc gtcttgcagg cccacctcga tatggtgccg caaaaaaata
     2821 acgacaccgt gcatgacttc accaaagatc ctatccagcc ttatattgat ggcgaatggg
     2881 ttaaagcacg cggcaccacg ctgggtgcgg ataacggcat tggtatggcc tctgcactgg
     2941 cggttctggc tgacgaaaac gtggttcacg gcccgctgga agtgctgctg accatgaccg
     3001 aagaagccgg tatggacggt gcttttggct tacagagcaa ctggttgcag gctgatattc
     3061 tgattaacac cgactccgaa gaagaaggtg aaatctacat gggttgtgcg ggaggtatcg
     3121 acttcacctc caacctgcat ttagatcgtg aagcggttcc agctggcttt gaaaccttca
     3181 agttaacctt aaaaggcctg aaaggcggtc actccggcgg tgaaatccac gttggcctgg
     3241 gtaatgccaa caaactgctg gtgcgcttcc tggcgggtca tgcagaagaa ctggacctgc
     3301 gccttatcga tttcaacggc ggcacactgc gtaacgccat cccgcgtgaa gcctttgcga
     3361 ccattgctgt cgcagctgat aaagtcgacg ctttgaaatc tctggtgaat acctatcagg
     3421 agatcctgaa aaacgagctg gcagagaaag agaaaaatct ggccttgttg ctggactctg
     3481 ttgcgaacga taaagctgcc ctgattgcga aatctcgcga tacctttatt cgtctgctga
     3541 acgccacccc gaacggtgtg atccgcaact ccgacgtggc aaaaggtgtg gtcgaaacct
     3601 ccctgaacgt cggtgtggtg accatgactg acaataacgt agaaattcac tgcctgatcc
     3661 gttctctgat cgacagcggt aaagactacg tggtgagcat gctggattcg ctgggtaaac
     3721 tggctggcgc gaaaaccgaa gcgaaaggcg catatcctgg ctggcagccg gacgctaatt
     3781 ctccggtgat gcatctggta cgtgaaacct atcagcgcct gttcaacaag acgccgaaca
     3841 tccagattat ccacgcgggc ctggaatgtg gtctgttcaa aaaaccgtat ccggaaatgg
     3901 acatggtttc tatcgggcca accatcaccg gtccacactc tccggatgag caagttcaca
     3961 tcgaaagcgt aggtcattac tggacactac tgactgaact gctgaaagaa attccggcga
     4021 agtaattatt tgatttgctg ccggatggcg tttaatcgcc ttccggcagt ttcatccttc
     4081 attatccttc gataaaagcc atccctgtga acgttcgtcg cgggttgcca cgttcaatct
     4141 ggtgatggaa cattcgccgc tgcgatttca gcgccgcgct attttcctgt tgctgttgtt
     4201 ccagcttcca ggcaatcagc aatcgcgcca gccgcttgtt agcatgccca gaaaccagtt
     4261 tttgcgccca tgatgaggcc gatacggact cggacaaata cactgaatag tgccgcacca
     4321 actttcgctt aatgcccatg cattatcgcc atccagagaa atcagcgccg aacgcagcgt
     4381 gtcagagtag cggcccgttt ctgtttccag caccgttacc gcgacgtctt gccgggtaaa
     4441 aacgaccaac agagaagaac gctgcgccaa tcgggtagcc gcgtccggga tgtaaatctg
     4501 gcatccccac aacgcgctgc atgttgggta aatttgccgt ggtgtgtaat tgttggatcg
     4561 ctaaactctc gatccacagg tcatcagatg cgatggtaaa taccgcatcg gataagggac
     4621 gaatatattt gcccatgtat cattccattt atactcgtca tacttcaagt tgcatgtact
     4681 gcgaccgcgt tcgttcaccc cagtcactta ctttagtaag ctcctgggga ttcactcgct
     4741 tgtcgccttc ctgcaactcg aattatttag agtatataaa tataaaaatc aggaaatggc
     4801 agacgaatcc agggcaagaa aaaaagaatt aatcctggaa aggtctgcaa agagtcattg
     4861 ataaaggatt tgtcatgatt tacctccttg cagtttgtgg aataagaaaa cgcgcacata
     4921 ccaggcgggt gttattttca ttgcaagctg gatttaatgt tgcggtttat atcgcataga
     4981 aaaattagta aaccacgctc ccctgcattc aacgtgctgc tgcttaactg tctgaaaacc
     5041 ataacgttca aaaaagggtt ttgcggttat gctggcgtcc accgtaagtt cggattcaga
     5101 cttaatcaaa ggttttaaca aggcgctggc aaccccgcgg cgggtgtatt cagggtcaac
     5161 aaataacata tcgatatagt gttcaacgca ggtaataaag ccgaccagtt ttgcattaat
     5221 gactgcaacc cgcacttgtg atttcgcgag tttctccttc cagcgagatt cgtcaatctg
     5281 cgcccaggcg gcaatttgtt gtggtgaata atgctgactg gcggtcatcg taaccgctct
     5341 aaggaaaata gcgcatagtt gctgaaaatc gccaggctga tagtctctta tttgtatgtt
     5401 attcataatc ccaacaccag ttgtctttcc atttgcgggt caagtaacgt cacatgcagc
     5461 cccaccagtc gcacaccgcg cccgccgcgg cgttcatccc aggttttacg cgcagtggcg
     5521 attagatcag ctttattcag ccgtggccag acgtgctcct gagtagtttg ctgaaaatcg
     5581 tcgaacttta atttcactcc ctgacgggcg atcagtaaat caggtttcac ctttgccaga
     5641 cggcgttcaa gttcaggata cagccgctcg ataatcgctt cacattcaga ccagtgatga
     5701 atatcttccg ccatcgtgcg ttccacgccg acggattttc gcaaccgttc actgttaacg
     5761 tcgcgctcgt caatcccctg actacgctcc cacaaaatgc ggccaaattt gccaaagcgt
     5821 ttaagcagaa tcaccagatc acacttttgc acatcaccgc aggtccgcag ccccatcgct
     5881 tccagttttg ccgccgagac tttgccgacg ccgggaattt ttgccagcgg taaggtttgc
     5941 agaaatgccg gaacttctgc cggcgtaatc acaaactgac cgttgggttt attcatgtcg
     6001 gaggcgattt tggcgagaaa ctttactggt gccacgcccg cagatgccgt cagttgcagc
     6061 tcgttgaaga tcgtctggcg gatttcctgg gcgatgaggg tcgcagaacc gtggcaatgg
     6121 acgctatcag tgacatcgag ataggcttca tccagtgaca acggttcaat acgcgaggtg
     6181 tagcgcgaga agatttcacg gatatgattt gaggcttctt tgtaggcgtc aaagcgcccc
     6241 ggaagcaagg tgagatgcgg gcataatttg agcgccatcc ctgtcggcat agcgctacgt
     6301 acgccaaatt tacgcgcggg ataattggcg gtgctgatta ccccccgtcg ttcgcggctg
     6361 ccgccaatag caatagggat atcgcgcagg gcgggattgt cgcgcatctc caccgctgcg
     6421 aaaaagcagt ccatatccac atgaatgatt ttacgcattg ctcacctctc aacactggta
     6481 aagtatacag tgatttcagg gtttgagaaa tgcgtaaaga ttcagcattt aacgcgctcg
     6541 ctgcgaaagc acctgctgct tatccagctt ttgcaccagc ggctgcacca ctttatcccc
     6601 atgctgacca aagtattgat acagcgtatc tgacgcactt ttggtcagca ccataatctc
     6661 aatgcgccgg ttgcccgcgc tttgcggatt tttggcatcc agcagcatct ggtccgccat
     6721 tgcgcttacc tgcatcactt tattttccgg cattccggcc tcttccagca cccgacgagc
     6781 cgaaagcgcg cgatcgcccg aaaggttcca gttgttgtag atattgtttt tgtaggccat
     6841 cgcatcggta tgcccggtaa taataatttt gttatcgagc gagtcgaaca ctggcgcgag
     6901 ctccaccagc agcgttttaa agaacggcat aatcttcgcg ctgccgcgtt caaacatatt
     6961 gcggttctgg tcatctttaa tcagcacgcg cagtccctgg ggaacaatct ccatctccag
     7021 attcgcttcc atatgtgcat cacgggcaat ggtgttgata ctggtcgcca gctcgccaag
     7081 ctcggtggcg gatttcttct tcagcagcgc cgttttctcg ttgacgtcgc gcgctttttt
     7141 ctccgtctct tccggcaccg ccacggtggc aggttttggc ggatgagacg ggctgatttt
     7201 atttaacggc gacaacccac caccgttaaa aatcgactgc ccgtgcagcg cggcgataat
     7261 gctttcacgc tcggatttac tgacgctatt gacaatcaac tttatcgtcg tcaccaaagg
     7321 ggccgagcgt atttccgagg tttccgcccg ctttacctta gacgcgatgc ccggtaagca
     7381 gatggcgatt gacgccgatc ttaacgccgg gttgatcaac caggcgcagg cgcaaacccg
     7441 gcgtaaagat gttgccagcg aggccgattt ctacggcgca atggacgggg catcgaagtt
     7501 tgtgcgcggg gacgccatcg ccgggatgat gattctggcg atcaacctga tcggcggcgt
     7561 ctgtatcggg atcttcaaat acaacctgag cgccgacgcc gccttccagc aatatgtgct
     7621 gatgaccatc ggcgacgggc tggtggcgca gatcccttcc ctgctgctct ccaccgcggc
     7681 ggcgattatc gtcacccgcg tcagcgacaa cggcgatatc gcccatgacg tgcgccacca
     7741 gctgctggca agcccgtcgg tgctctacac cgccaccgga attatgtttg tactggcggt
     7801 ggtgccggga atgccgcacc tgccgttttt gctgttcagc gccctgcttg gctttaccgg
     7861 ctggcggatg agcaaacgcc cgcaggcggc ggaggcggag gagaaaagtc ttgaaacgct
     7921 aacccgcacc atcactgaaa ccagcgagca acaggtcagt tgggaaacca ttccgctgat
     7981 cgagcctatc agcttaagcc tcggctacaa gctggtggcg ctggtcgata aagcgcaggg
     8041 gaacccactc acccagcgga ttcgcggcgt gcggcaggtg atatccgacg gcaacggcgt
     8101 gctgctgccg gagatccgta ttcgggaaaa cttccgcctc aagcccagcc agtacgccat
     8161 attcatcaac ggcattaagg ccgatgaagc ggatattccg gcagataaac tgatggccct
     8221 gccctccagc gaaacctacg gcgagattga cggcgtactg gggaatgacc cggcatatgg
     8281 gatgccggtc acctggattc agcctgcgca gaaagcgaag gcgctgaata tggggtatca
     8341 ggtgatcgac agcgccagcg tgattgccac ccatgtgaat aagattgtgc gcagctatat
     8401 tcctgattta tttaactatg acgatattac gcagttgcat aaccgcctgt cgtcgatggc
     8461 accgcgactg gcggaagact taagcgcagc gctcaattac agccagttgc tgaaagtgta
     8521 tcgcgcgctg ctgaccgaag gcgtttccct gcgcgatatt gtcaccatcg ccaccgtgct
     8581 ggtcgccagt agtgcggtga ctaaagatca tatcctgctg gcggcagatg tgcgcctggc
     8641 gctgcggcgc agtatcaccc atccgttcgt tcgcaagcag gagctgacgg tgtatacgct
     8701 gaataatgag ctggaaaatc tgctgaccaa cgtggtgaat caggcgcaac aggccggcaa
     8761 agtgatgctc gacagcgtgc cggtggaccc gaatatgctt aaccagttcc agagtacgat
     8821 gccacaggtg aaggagcaga tgaaagcggc ggggaaagac ccggtgctgc tggtgccgcc
     8881 gcaactgcgt ccattgctgg cgcgctatgc aaggttgttt gcgccggggc tgcatgtgct
     8941 gtcgtataac gaagtgccgg atgagctgga gttgaagatt atgggggcgt tgatgtaaga
     9001 atccggcatc ggtgcacaat tgccggatgc ggcgtgaacg ccttatccgg cctaccgtta
     9061 aggcatagat ttgtaggcat gataagacgc gctagcgtcg catcaggcat ctgcgcacaa
     9121 ccgccggatg cggcgtgaac gccttatccg gcctaccgtt aaggcacaga tttgtaggcc
     9181 ggataagcgt aagcgcatcc ggcaccggcg cacaattata aaataatacg ctccccggca
     9241 ctaaaatccg ttacgtcccc cgcccaatcg atgggatata aacctttcgc gacatcgcga
     9301 tggaacgttg agaatggcca atcactcacc tgctttaccc agccatgttt cactggattt
     9361 atgtaaatat aatcaacatg atgacgataa tctttggtat tgcgaatggc atgctcccaa
     9421 aaacgcggtt gccacatatt ttgcaatcca caagcatggg tgaactgttt tttaatctcc
     9481 cgccagcgag aggaaaaatc atcatcgctt tcgggtaatg tccagataca gtgcatatgt
     9541 tccggcaaaa cgacccaggc gttaatttca aaaggtctgg cctgcttaac tttaataatg
     9601 gcgttacgga gtgtctggta ctgggtggtc aaaagttggc ttcgacgatt ccgtaaattt
     9661 accgtgaaaa accatgttcc ccccttgatg taataacgac gatattcaga catggcgact
     9721 cctttttctc aacgataaga agaaaaggaa aagtcgacaa tgaagttgtg ttatatcggg
     9781 aaagcagctc gcaaaaaatt cgcagactcg taggcatgat aagacgcggc agcgtcgcat
     9841 caggcatcgg tgcactattg ccggatgcgg cgtgaacgcc ttatccgtcc tacaaaatcg
     9901 tttcctccgt caaaatcctg cgcgcgccta aaaaatggtc ctgccagaaa ggttcggcta
     9961 accgacttac ccgaatggtt tccccggtgc gcggtgactc gataaactga ccgtctccaa
    10021 gatatacgcc catatggtcg gctatctcac gactgtggat atggaaaaac agcaaatctc
    10081 cccggcgcag gtcgttattc gccacaatcg ttgcccgatg gtagtgatac atctcattgg
    10141 ccgtacgcgg gagcttcgcc tcgaggatct tgttgtaggc ataaaaaacc aacccgctac
    10201 agtcgaagcc ttgatcgggc cgcgtgccgc cccagacgta aggcttacca agctgctgct
    10261 ccaggcggtg aatcgccact tcggtgatgt tgtgtagatg gtcgctgctt aagaaatggt
    10321 tgttctccgc cagcgcctgc cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt
    10381 ctggatgctg ttttatctgc tcccgattat gctggcgcag cgcccttctg cttttggcat
    10441 tgccttccac aatatagctg gcctgctttt tcagatgggt ttggtactgt tttaataaac
    10501 gcgcacggtt ttgcatccgc tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg
    10561 cgctggcgag taaagggagc aaaacgcata acgaaaacac gcctggatgc agaaatctgc
    10621 cgtgcaagaa cgaagacatg aaggacataa accagctgaa tgaaggtgag aattttaaaa
    10681 gaccggagga gtaaacggga ttattaattg cttatttgca tattactatt gcggtttcat
    10741 aatcttagga aataagaaat aaatctacaa tccatgcgct tttcggactc ttgaaaactg
    10801 cccctgtgct aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc ttttggtgat
    10861 aatgtgagcc ttccaaatcc agactattcc gatttgggat tcattttgcc gtcctggcgt
    10921 gtgtaaccgt tttatcaagg aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg
    10981 ttgttgatcc cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc gaccacgcca
    11041 gtgagcaaag agtataagca gcagttgatg ggatcccctg tctacatcca gatcttcaag
    11101 gaagaacgta cgctcgatct ctacgtcaaa atgggcgagc aatatcaact gctcgacagc
    11161 tataaaatct gtaaatattc cggcggctta gggccaaaac agcgtcaggg cgatttcaaa
    11221 agcccggaag ggttttatag cgtccagcgt aatcagttaa aaccagacag ccgttactac
    11281 aaagcgatta atattggatt cccaaacgcc tatgaccgtg cgcatggtta tgaagggaaa
    11341 tacctgatga ttcacggcga ttgtgtttcc atcggctgct acgcgatgac caatcagggt
    11401 attgatgaga tcttccagtt cgttactggt gcgctggtgt ttggtcagcc gagcgtgcaa
    11461 gtgagtattt acccgttccg tatgaccgac gccaatatga agcgccataa atattccaac
    11521 tttaaggact tctgggagca actgaagcca ggctacgact actttgagca aacccgtaag
    11581 ccaccgaccg tttctgtggt caatggccgt tacgtggtca gcaagccgtt aagccacgaa
    11641 gttgtgcagc cacaactggc atcaaactac acgctccccg aggcaaaata agcgccattc
    11701 acctggcata atcttttgcc aggtttcatt gcccgtcagc ggctgtgtcg caatcaccgt
    11761 gaccacatca ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct gatccagcaa
    11821 cgttgccacg ccaaacggcg cgcggcgggt gatccagtgt aaattagtcg agcaatacgc
    11881 cattacatag cgcccgtcag aaagcagcat gttgaaaacg cccttctgcc gcagttcatc
    11941 cgccagtgag gcgatatatt taaacactgc cgccatgttg cccggtgtgc gcgggtaacg
    12001 ctgcgttaat ttatgcagga gccagcaaaa ggctttttcg ctatcggttt caccgaccgg
    12061 gcggaagttg ccggtttcca gtgatttgta gcccgtcagt tgtccattat gggcataagt
    12121 ccagttacgc ccccataact cgcgggtaaa cgggtgagta ttttccagcg ccacctcgcc
    12181 ccgattagcc tggcgaatat gagccaccac cgaacaggat tttatcgggt agtcctggac
    12241 aagtttggcg atgggggaat taaagctggg ttgcggatct ttaaatgtgc gacagccttt
    12301 accttcgtaa aaggtaatgc cccagccatc tttatgtggc ccggttccac caccacgctg
    12361 tacaagtccg gtgaaactaa agcagatatc ggtagggacg ttggcgctca tcccgagcaa
    12421 ttcgcacatg ggtaacctcc acaacagcgg gagcgcatgg cccccggcga agattgatta
    12481 aaaagtacaa aggttcaggc tgttaccctc accccggccc tctccctgaa agggagaaaa
    12541 gaccttactt aaccatctct ttttcaatta actggatcag gatatggatc actttaatgt
    12601 ggatctcctg aatgcggtcg gcataaccaa agtgcggtac gcgaatttcg atatccgctg
    12661 tgccagccat tttgccgccg tctttaccgg tcagggtgat cactttcatc cccttctcac
    12721 gcgccgccgc gatcgctttg atcacgtttg cagagttacc ggaggtggag atccccagca
    12781 gtacatcgcc ttcgcgacct accgcttcaa cgtaacggga gaaaatatca ttgaaaccaa
    12841 aatcgttacc gacgcaggaa atatggctga cgtcagaaat ggcaatcgcc gggtagcccg
    12901 gacggttttc acggtagcga ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg
    12961 agccgccgtt gccgcaggaa agcactttgc caccggcttt aaagctgtct gctaacagga
    13021 ccgccgcgcg ctgaatggcg tgaatattgg cgtcatcttt taaaaaatta gccagcgttt
    13081 ccgccgcttc gttcagttcg ttacgaataa gatcctggta catgaggata tccttcagca
    13141 taaatgtaat agacaaaatg cagtgtaccg gataccgcca aaagcgagaa gtacgggcag
    13201 gtgctatgac caggactttt tgacctgaag tgcggataaa aacagcaaca atgtgagctt
    13261 tgttgtaatt atattgtaaa cgtattgcta aatgttttta catccactac aaccatatca
    13321 tcacaagtgg tcagacctcc tacaagtaag gggcttttcg ttatgatgat tttgagtatt
    13381 ctcgctacgg ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt atttatcagc
    13441 agtctgattt tgctcgcctg gacagccgca ctcggcgttg ctggtctgtg gtcggcgtgg
    13501 gtactggtgc ctctggccat tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg
    13561 atgatttccg cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact
    13621 gagaaagaag cgattgatgc gggcaccacc tggtgggaag gcgatttgtt ccagggcaag
    13681 ccggactgga aaaagctgca taactatccg cagccgcgcc tgaccgcaga agagcaagcg
    13741 tttctcgacg gcccggtaga agaagcctgc aggatggcga atgatttcca gattacccat
    13801 gagctggcgg atttgccgcc ggagctgtgg gcgtatctta aagagcatcg cttcttcgcg
    13861 atgatcatca aaaaagagta cggcgggctg gagttctcgg cttatgccca gtctcgcgtg
    13921 ctgcaaaaac tctccggagt gagcgggatc ctggcgatta ccgtcggcgt gccaaactca
    13981 ttaggcccgg gtgagctact gcaacattac ggcactgacg agcagaaaga tcactatctg
    14041 ccgcgtctgg cgcgtggtca ggagatccct tgctttgcac tgaccagccc cgaagcgggt
    14101 tccgatgcgg gcgcgattcc ggataccggg attgtttgca tgggcgagtg gcagggccag
    14161 caggtgctgg ggatgcgact gacctggaac aaacgctaca ttacgctcgc gccgattgcg
    14221 accgtgcttg gtctggcgtt taaactctcc gacccggaaa aactgctggg tggcgcagaa
    14281 gatttaggta ttacctgtgc gctgatcccg accaccacgc cgggcgtgga aattggtcgt
    14341 cgccacttcc cgctgaacgt gccgttccag aacggaccga cgcgcggtaa agatgtcttc
    14401 gtaccgatcg attacattat cggcggaccc aaaatggccg ggcaaggctg gcggatgctg
    14461 gtggagtgcc tctcggtagg ccgcggtatc accctgcctt ccaactcaac cggcggcgtg
    14521 aaatcggtag cgctggcaac gggtgcgtat gcgcacatcc gccgtcagtt caaaatctct
    14581 attggtaaga tggaagggat tgaagagccg ctggcgcgta ttgccggaaa cgcttatgta
    14641 atggatgctg cggcatcgct gattacctac ggcattatgc tcggtgaaaa acctgccgtg
    14701 ctgtcggcta tcgttaagta tcactgtacc caccgtgggc agcagtcgat tattgatgcg
    14761 atggatatca ctggtggcaa aggcattatg ctcgggcaaa gtaacttcct ggcccgtgct
    14821 taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac ccgcagcatg
    14881 atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagcg
    14941 gcgaagaaca atgacgtaaa tgcgttcgat aaactgctgt tcaaacatat cggtcacgtc
    15001 ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg
    15061 ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag cgccaacctc
    15121 gccctgcttt ctgatgtctc gatggcggtg ctgggcggca gcctgaaacg tcgtgagcgt
    15181 atctcggcac gtctggggga tattttaagt cagctttacc tcgcctctgc cgttttgaag
    15241 cgttatgacg acgaaggccg caacgaagcc gacctgccgc tggtgcactg gggcgtacag
    15301 gatgcgctgt atcaggcaga acaggcgatg gacgatttac tgcaaaactt cccgaaccgc
    15361 gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctggcacct
    15421 tctgacaagc tggatcataa ggtggcgaag attttacaag tgccgaacgc cacccgttcc
    15481 cgcattggtc gcggtcagta cctgacgccg agcgagcata acccggttgg tttactggaa
    15541 gaggcgctgg tggatgtgat tgccgccgac ccgattcatc agcggatctg taaagagctg
    15601 ggtaaaaacc tgccgtttac ccgtctggat gaactggcgc acaacgcgct ggcgaagggg
    15661 ctgattgata aagatgaagc cgctattctg gtgaaagctg aagaaagccg tctgcgcagt
    15721 attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa gttgccggag
    15781 aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt
    15841 atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc
    15901 cgcgaacagc accgttttac catcaataga aagcgcatcg ttcacattca accaggtgag
    15961 tttctcttgc gacgttttct catcaatagt cgagaacagc cccgtcatct gattagattt
    16021 ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat
    16081 cacctgatac gtctcatctc ccaatgtcac ggtttgtgcg ggagtataag taccgccttt
    16141 catcacccag gcaggcagct tatgcccttg caccatctga ttaaacgccg ctttggtggt
    16201 ttcgcccttt gcaagactgc taatggttaa atcatcctgc gccattgcac tggtggcgat
    16261 gaccagagcg gcgactgtcg ttattgcctt aaacatcatt cctcccgggc ttatcctgcc
    16321 catgtgatta actccctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc
    16381 aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttag
    16441 agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt
    16501 ttgcagcaac cactggtgtg gatggatggt cctgccaacc tgcgccattt tgatcgtcaa
    16561 ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac cagcggcttt
    16621 gccatggaag cggcagcttc gtcgctagca caagatgacg tagtgaactg gatgaccgct
    16681 aaagcgcagc agtgcaatgc gctgattgca ggcagtgttg cattacaaac ggagtctggt
    16741 tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta tgataagcgt
    16801 catctgttcc gcatggcaga tgagcatctg cattataaag cgggcaatga gcgagtgatt
    16861 gtggaatggc gcggctggcg tattttgccg ctggtgtgct acgacttacg ttttccggtg
    16921 tggtcgcgca atctcaacga ttatgacctc gccctgtacg tcgccaactg gcctgctccg
    16981 cgctctctgc actggcaggc attgctgaca gcccgcgcga ttgagaatca agcgtatgtg
    17041 gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagccgg
    17101 gtgattaatc cgcaaggcga gattatcgct actgccgacg cgcatcaggc aacgcgcatt
    17161 gatgcggagc tgtcgatgat ggcattgcgg gaatatagag aaaagtttcc ggcatggcgg
    17221 gatgcggatg agtttaggtt gtggtgagtg ggggttgtgt ttagggctgg ggagagtcag
    17281 ggtaagacta cgaaagcccg ctcccccagg gaaagattac gaaagc
//
LOCUS       DAZKJR010000110        17287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKJR010000110 DAZKJR010000000
VERSION     DAZKJR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.111"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..381)
                     /locus_tag="ACHOWS_003675"
     CDS             complement(<1..381)
                     /locus_tag="ACHOWS_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFQ0049194.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFLWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVIFSLESV
                     FILILSVILFSVKINSPLHFILLLT"
     gene            complement(374..556)
                     /locus_tag="ACHOWS_003676"
     CDS             complement(374..556)
                     /locus_tag="ACHOWS_003676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049195.1"
                     /translation="MIFNWNVLFYVSLEKFPVGNPYYGEIPGIPVDEEAKFFNPFPPR
                     AFENKPLYTEEITKYD"
     gene            complement(596..796)
                     /locus_tag="ACHOWS_003677"
     CDS             complement(596..796)
                     /locus_tag="ACHOWS_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049196.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGELA"
     gene            915..1262
                     /locus_tag="ACHOWS_003678"
     CDS             915..1262
                     /locus_tag="ACHOWS_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFQ0049197.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1280..1870)
                     /locus_tag="ACHOWS_003679"
     CDS             complement(1280..1870)
                     /locus_tag="ACHOWS_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049198.1"
                     /translation="MTQSRQPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPATGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWKKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(1870..2127)
                     /locus_tag="ACHOWS_003680"
     CDS             complement(1870..2127)
                     /locus_tag="ACHOWS_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049199.1"
                     /translation="MDEERVFSLSYEQLTRLAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            2724..3137
                     /locus_tag="ACHOWS_003681"
     CDS             2724..3137
                     /locus_tag="ACHOWS_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049200.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLDGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            3139..3918
                     /locus_tag="ACHOWS_003682"
     CDS             3139..3918
                     /locus_tag="ACHOWS_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0049201.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            4098..4742
                     /locus_tag="ACHOWS_003683"
     CDS             4098..4742
                     /locus_tag="ACHOWS_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0049202.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            4829..5137
                     /locus_tag="ACHOWS_003684"
     CDS             4829..5137
                     /locus_tag="ACHOWS_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFQ0049203.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            5550..6530
                     /locus_tag="ACHOWS_003685"
     CDS             5550..6530
                     /locus_tag="ACHOWS_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFQ0049204.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGADIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            6523..6939
                     /locus_tag="ACHOWS_003686"
     CDS             6523..6939
                     /locus_tag="ACHOWS_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFQ0049205.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(6941..8215)
                     /locus_tag="ACHOWS_003687"
     CDS             complement(6941..8215)
                     /locus_tag="ACHOWS_003687"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0049206.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAIEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(8215..8652)
                     /gene="umuD"
                     /locus_tag="ACHOWS_003688"
     CDS             complement(8215..8652)
                     /gene="umuD"
                     /locus_tag="ACHOWS_003688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFQ0049207.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLHPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(8649..8897)
                     /locus_tag="ACHOWS_003689"
     CDS             complement(8649..8897)
                     /locus_tag="ACHOWS_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFQ0049208.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSLSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            9315..10217
                     /locus_tag="ACHOWS_003690"
     CDS             9315..10217
                     /locus_tag="ACHOWS_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFQ0049209.1"
                     /translation="MYFSAEPAQITEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIKVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            10214..10525
                     /locus_tag="ACHOWS_003691"
     CDS             10214..10525
                     /locus_tag="ACHOWS_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049210.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRT
                     R"
     gene            10601..10672
                     /locus_tag="ACHOWS_003692"
                     /pseudo
     CDS             10601..10672
                     /locus_tag="ACHOWS_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10754..12454)
                     /gene="ltrA"
                     /locus_tag="ACHOWS_003693"
     CDS             complement(10754..12454)
                     /gene="ltrA"
                     /locus_tag="ACHOWS_003693"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0049211.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWVRYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            13076..13687
                     /locus_tag="ACHOWS_003694"
                     /pseudo
     CDS             13076..13687
                     /locus_tag="ACHOWS_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            13688..13909
                     /locus_tag="ACHOWS_003695"
     CDS             13688..13909
                     /locus_tag="ACHOWS_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049212.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            13869..14355
                     /locus_tag="ACHOWS_003696"
                     /pseudo
     CDS             13869..14355
                     /locus_tag="ACHOWS_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            14400..15170
                     /locus_tag="ACHOWS_003697"
     CDS             14400..15170
                     /locus_tag="ACHOWS_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049213.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNSHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            15584..16009
                     /locus_tag="ACHOWS_003698"
     CDS             15584..16009
                     /locus_tag="ACHOWS_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFQ0049214.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            16056..16475
                     /locus_tag="ACHOWS_003699"
     CDS             16056..16475
                     /locus_tag="ACHOWS_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049215.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVIQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            16475..16666
                     /locus_tag="ACHOWS_003700"
     CDS             16475..16666
                     /locus_tag="ACHOWS_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049216.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            16680..16981
                     /locus_tag="ACHOWS_003701"
                     /pseudo
     CDS             16680..16981
                     /locus_tag="ACHOWS_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16981..17076)
                     /locus_tag="ACHOWS_003702"
                     /pseudo
     CDS             complement(16981..17076)
                     /locus_tag="ACHOWS_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3908 a         4340 c         4585 g         4454 t
ORIGIN      
        1 agtaagcaat aatataaagt gtaatggtga gttaatttta acggaaaata atataacaga
       61 tagtataaga ataaatactg actcaagtga aaaaataaca gctgttatac cagcagaaat
      121 aaattttagg ccatagagag tgaatgtata cgctagaaat ggctcaaaaa ttcctgttga
      181 ataggcaagg attgattttt ttgttaccgg gagttttttt ctgctaattg cgagaacaaa
      241 ccatgataac attgcagcac taccaacttg ccataaaaac aactctccgg catccatgtt
      301 ttcgagacct tttttgctca tcactattgc tgctccccat gaaaaagcag caagaaatgc
      361 aaacataatt cccttaatca tatttagtta tctcctcagt ataaagaggt ttattttcaa
      421 acgctcttgg tggaaatgga ttaaaaaact ttgcttcttc atccacagga atacctggaa
      481 tttctccata ataaggattg ccaacaggaa acttttccag tgagacataa aacaatacat
      541 tccagttaaa aatcatagga tcattactat caacatcatg gattatgatg gcgtcctaag
      601 ccagttctcc aggaattaat acatctgtat gataaaaacc tgaatagtaa tcactattag
      661 tagaatgtat agggcaagca aagcgattca ttttagtatc tgagtgccat gtggttgata
      721 ttttttctgg tgcgggaatg atgaaatctg ctatcataat attaagaaaa tactttacag
      781 atatatccag tttcatctgg tttctccttt attgttatcg tgtaccggtg catgacaaca
      841 taatcacagc atgataataa taatcaataa caataagctg tgtcacgttt acatcatgga
      901 acggaggtgc tcctgtgacc tttgagcaga aaaaagcccg ggccattgcc ctgatggata
      961 gcaaaaagat gtggcggagc aattatgccc ctcctcttct gcgtattctc tggcggctgg
     1021 gtatccggct tccgccatta ccctttatgc cgttctggca ggtcacgctg ttgatgggga
     1081 gcttatgggg catttcctgg ggatgtgcaa tgtggtttat ctactggggg ccgtcaggaa
     1141 tggtggcagg tgaggccatt attataagta tcacgggtgg ttttttgttc ggactgctca
     1201 cggcctcttt ccactggtgg cgcaggaaag ttaaccggct gccaccatgg gatgatgtgt
     1261 aaaccgtgca ggcatgtttt tacggggtga tgctttcatt aacctgcaca cggacctcca
     1321 ttcctgcatc gtgacaggcc tgcagccact gcgccacttc cagcggatcg ccctcccggc
     1381 gtaccactct gccttctttc ttccataact gcagacaggt gctgccgtcg agacgcacca
     1441 caaaatcccc acggcaggcc tgatacgtta tgccgttcag ctccaccgcc agatcagtgg
     1501 cagaagtgcc gtctgcgggc agacctgttg ccgggatcag cggcagtacg acgacttctg
     1561 ccgcacgctg ttctgcccgg aggcggtcct gttctgccag cagcagaggc tgtgccacgg
     1621 cccggccggc gtccgtcagc tccactgcca gctgcaggtt cggagcgcgc agggtacgca
     1681 gccagccggc atcctccaga cgacggcagg aggcgcgcag gtttggcccg tagaccgggg
     1741 cttccccgct tcgctccagc acccgttcta tgtcccgcgt cagcacgggg cctggccgtt
     1801 tttcatcaag ggcagccagc acaatcagca ctctccgctg cagtggcgac ggttggcggc
     1861 tctgggtcat tacctgtcat cctccggcca gatgaggttt ctgaggcgct gaaagtcggc
     1921 gtcaatgagc tcctgtctct ttatggcatt cattgaggca tagccattta tggccagttc
     1981 atgccagaag atcagcacgg ctccggcttt ggctgactcg cagagataaa tcgcgccctg
     2041 gctgtccaga ttgcattccc ggatgcgttt ttccgcaagc cgggtcagtt gttcatagct
     2101 gagtgaaaac accctttctt catccatcct gcgcgctcca tctgataatg ttctgacagg
     2161 taaaaatcaa attctatatg atttttgtgt tattgatttc tgataatatc agatattgtt
     2221 tcattacgga tgattaaaat catgtctgat atgaatttat gtgtgttgct taattatcat
     2281 atcgcctgcc cgtttcccgc cgcgcagatc cggtttccgg cttttctgcc gcgcagatcc
     2341 gaaaaccctg agtcacggat cttcctctcc ctcacacaac gccatctcct gtcagtacaa
     2401 catgtcgtgc tgcattcagc tgctcatgac actatatatt gtgttatttc cctgacctgt
     2461 gatgcgtcgc gcaggggcgg aaaacagcga tatgattatt tccccagcgt ggtacacttc
     2521 cggaaagttg tgatattcag gaaagtcgga tctgacggaa acggctctcc ggtaatttaa
     2581 cggcgtggtt atatggatgc ttgttatcat ggtgatgatg ataacggcat gatgttatca
     2641 gacggcgtga cggtaagggc agtgatgatg gatgacgtta tcgcatgacc gtccctgccc
     2701 ggaaaagaaa aaaggagtca cccatgtttt ttattgagaa tgaaggtcag gctgtcgcca
     2761 gaacggatta ctggcagtct gtacaggcgc aggccggata tgtctacctc agctggaatg
     2821 ccggcgcagc caggctgctt gtcccggatg cggcaaaaca tttactcagg gagatgcggg
     2881 gggctgagta cgtcatcatc agtaagggaa cgctggatgg ccgcgatgcg ctggaactga
     2941 tatttgaaga cggcagcgat gcgccgtttg tgatccacat gctgagtgag cagtgcgatc
     3001 gcctgctccc cgaaaacaac cagggagggg gttttgttgt caccgtctgg acgcgtggcg
     3061 gtaaccagct ccgttatccg ggaaaatacc gggttgtgga aaacctgccc gacgtttccc
     3121 cgtggagtga gcactgatat gcagcacctg ccggcaccga tccaccatgc ccgggatgcc
     3181 gctcagcttc ctgttgccat cgattatccg gcagcgctgg cactccgcca gatgtcgatg
     3241 gttcatgatg aactgccaaa atacctgctg gcccctgaag tgagcgccct gctccattac
     3301 gtgccggatc tgcgccgcaa gatgctgctg gccacactgt ggaacaccgg tgcgcgcatt
     3361 aatgaagcac tggcgctgac gcggggggat ttttcgcttg cgcctccgta tccgtttgtg
     3421 cagcttgcga ccctgaaaca acggacggaa aaagccgcca ggacggcagg aagaatgccc
     3481 gccggtcagc agactcaccg gctggttccg ctctccgacg cctggtacgt cagccagctg
     3541 cagacgatgg tggccacact gaaaatcccc atggaacggc gtaataaacg aacaggcagg
     3601 acagagaaag cgcggatctg ggaagtgacg gacagaacgg tcaggacctg gattggggag
     3661 gcggttgccg ccgctgctgc tgacggtgtg acgttctctg tcccggtcac gccacatacg
     3721 ttccgccatt cctatgcgat gcacatgctg tatgccggta taccgctgaa ggtcctgcag
     3781 agcctgatgg ggcataagtc catcagctca acagaggtct acacgaaggt gtttgcactg
     3841 gatgtggctg cacggcaccg ggtgcagttt tcgatgcctg agtccgatgc ggtgtctatg
     3901 ctcaaaagaa ttccttaagc tctgactttt ttaaatccct ttcggatatc tcatggatat
     3961 attatcggta tttcatattc tgttgacatg caggaccggt atagagtaaa aaaacatcta
     4021 ttgagtacct cttggatatc cgaaaggtgt cttagggggt ctttatgggt attacatgtg
     4081 gattctggag atacactatg cctgttattg ctaatacgca tcccaaaggt ggtgtgggta
     4141 agacaacttc atccgtaaac attgttggtg aaatgaagtc tgatacagtt gatctggata
     4201 ctcataccgg gctttctatc attctggggc tgagacctga aggaaaagaa atttccgtga
     4261 aagtacccaa aacagtggat gagttaatcg aaattatgac tccctacaag aacagcgata
     4321 agacgttact tattgactgt gggggatttg actcagatct tacccgtact gcgattgcct
     4381 ttgcagactg cgttattgtc ccttcaaaag actccctgac tgaacgtatt ggtctgatgc
     4441 attttgatgg agtactggac gaaatcagct ctatcatggg gaccgatata actgctcacc
     4501 tgtatctctg taaagttaat cctaacaaga agaaattccc taagcttgat gcaatcctgc
     4561 catcgttcaa acatctgaag ctgatgaaga gccgcatttc tgctcgtgca gaatttgagg
     4621 atgtcattga gactgggatg ggaatcactg agtctgttca tgggcgctat tcggcaggag
     4681 gtaaggaagt tattgccctg atcgaagaaa tcaatcatct gattgaaaat aacaaacagt
     4741 aaggtgtatc tattgggtat tcatatagta tcctttgggc ttttaatgga tatcttttaa
     4801 gtatctaaca aacatcctgg gttatctcat gagcaaagcg aaatttagtg cacaggctga
     4861 aaaactgaaa aacctggtgg attctgccag attacaaact tcttcttctg ccgtaacagc
     4921 aacagccccg tctcagccaa atgaaggtgc agaggaagtt tcaggtcgtc ccgtggctgc
     4981 caggacgaag gctaagaatc ttaaggccat accgctgtcg tattttgaag ctcatgcgga
     5041 acttaaagcg accagtaaaa catctctgga ttttagctca tatattattg aggctataag
     5101 agagaagctt gaacgtgatg gtgcaatagg tcaataaact atcggatacc ttaagggtat
     5161 ctgatgtgtg tcttttaaat gcgttatggg gattcttcac gtacctaaag agtccccatt
     5221 agacatcttt ctgtttatgg tgacatgatt cgtcaccgct ttcctttcct ctcagtttca
     5281 ccatttcatt cctttccctt aacctgaacc atgatgtcca cgatgttttt atattgcatt
     5341 tatgtatcac atataacctt cataatgttt ttgtatctat gatttcctct ggtcttaaag
     5401 ttgtgcgtac aatgtacatg caatttatcg tgtttttatg ttgcatttgt cctttcagaa
     5461 agaccgtttt ttcattataa ttgaatatac attgcatgta tgaagcatgt ttgttttttc
     5521 tatttgttta tgagcaacgg agactggtta tgaaaatctt tattgatgat ggttctacca
     5581 acattaaact tgcctggttg gaggatggtg acgtgaaaac gttaatcagc cctaacagct
     5641 ttaaaccgga atggtctttt agtttactgg atgatgctgc tccggctaat tacgagatcg
     5701 acggcgagaa gttttcattt gatccattaa gtgctgatgc tgtcgtaacg actgaaacac
     5761 gctatcagta cagtgatgtg aatgttgtgg ccattcagca tgcgctacag cagaccggcc
     5821 tgaaagcgca gcctgttgat gtcatcgtca cattgccgat ctctgaatac cttgacgcta
     5881 acaatcagaa aaataagcaa aacattgaaa gaaagaaaaa aaatgtgatg cgtgaagtac
     5941 gggtgcaggg gagtgatgca tttgttattc gcagtgtttc cgttcttcct gaaagtattc
     6001 ccgctggttt cagtgttctt gcggggcttg aggatgatga atcccttttg atcgtagatc
     6061 tcggtggtac aactcttgat gtgtcgcatg ttcgtagcaa aatgacaggg attacaaaaa
     6121 cctggtgtga tccgaatatc ggcgtgtccc tgattacatc cggggttaag gaacagatgg
     6181 ctgtacatgc taatacaaga gtgagctcat tccaggctga caatatcatt gtgcaccgta
     6241 atgaacctga ctacctttcc cgtcgcattt ataatgctga acagcgtgag tctatcatta
     6301 atgttatcaa tgaacggcag aagttgctta ttaaacgagt taatgatgtc atcagccgtt
     6361 tcaccgacta tacacatgta atgtgtgttg gtggtggggc tgacattgtg gccgaggctg
     6421 tgaagaacct gactaaggtt cctgatgagc gcttttatct gagctcatcc ccgcagtttg
     6481 atttagtcat gggaatgata aaaatgaaag gtggtgtgac taatgagtga tgaaaataaa
     6541 tccagacgct gctcatttga acttttcccg gatgaacgga caggagataa gatagcagat
     6601 gagctcattg caaatgaaaa actgaaggag cggggacgtt tcatgcgcgc gatgttagtt
     6661 actggtgcgg cgtttgcagc tatagataaa cgtcttcctt tgctgatatc ggagttactg
     6721 acagaaaata cgactctgga tgatattaat aaggtgattt ccagcgtaat accaggtgct
     6781 ttttcggttg aaaagaaatt gcttgaatta ctggaaaaac aatctggtct ccatacgtct
     6841 gtggattgca gtactcctct tacagagcag agtctgtcga gaaatgacgg tgaagaccag
     6901 accagaagaa acgctgaaaa tatgttcgga gatgactgaa ctaacccagc ctggcaaggg
     6961 gaatatctcg ccatttagtt gtataacact gactcaacat ttcccgtttc atttgccatt
     7021 caggggcggt tcctcgcccc gcaaaccaca ctttcccttt tcccgactgg ttcagttcat
     7081 cgagtgtttt cattaacttt tcactgtttt tacggggctg gatttcatca aataatcccg
     7141 gctgcgctat acccgatggt gtgaaatcag ccagcatgac tcctgccttc atatagcggt
     7201 acccttcacg ccagacatgg tttaaggctc tgcatgcggc ggcaataatg tcccggctgt
     7261 cctgtgtggg caatggaagc ttttccacag cggcattgct gtaacagggt tcttttactg
     7321 caaaggggga tgtccgtaca aatgtcgtca cctgccggca atactgacgc tccccacgta
     7381 gtttctctgc ggcccgctct gcatactgaa caacagcctg gtgcatggca tctttgtctg
     7441 tgattcgttc accaaaactg cgactacaga caatctgctg ttttgccggt ggtgcttctt
     7501 ccagggatat gcaggactcg ccgttgagtt cgcgtaccgt acgctcaaga atgacgctga
     7561 agtttttccg gatgaatgcc gtgttagcct gcgccagctg cagtgctgtg ttaataccca
     7621 gcgcattcag cttttccgtc agtctgcgtc ctactcccca gacctcacca actggctgca
     7681 gccccagtag cttcaaggtc cgattacggt tttctgccgt cagcgcgacc actccggaaa
     7741 actgtggcca ttgctttgtt gcccactgtg cacttttagc cagcgttttt gtaggcgcaa
     7801 tgcccacccc catggtgagt cctgtccagc tctttacctg ttccctgagc tgatgaccaa
     7861 aaaactccgg agagatgcaa tgatttatcc cccgcaaatc aataaacatt tcatcaattg
     7921 agtagggctc aattgcggga gaaagcgact ccagaacagc catgacccgt tggctcatgc
     7981 tgtggtacag cgcataattg ctggaaaata catgtatttt cttctccagg cgcatctgtc
     8041 tcacctgaaa ccagggctgc cccattctga tgccaagggc ttttgcctcc gggctgcgcg
     8101 cgatcacaca gccatcgtta ttgctgagta cgatgaccgg ttcgttgcga aggtccgggc
     8161 ggaaaacttt ttcacatgag gcgtagaaac tgttgatatc agccagtgca aacatcagcg
     8221 taactcccgc gtcctgtgta tcacgtgagt gacaacacca aaaatacaga tgttttccgg
     8281 atacagtgtg cggaactccg ggctgtctga aaccggctcc agtgccgggc gtgggtgcaa
     8341 cagcagtcgt ttgacggtga actccccgtc gatctcagcg ataacgatgt ccccgtgttg
     8401 tggtttttcg gccctgtcca ctaccagcag atcaccattc tgcacgccag cctggttcat
     8461 cgattcaccg ctggcgcgca gaaagaaggt ggctgcaggt ctgctgatgc aatagctgtt
     8521 cagatccagt tcctgctcag cataatcagt ggcaggagaa ggaaaaccgg cctggcaacg
     8581 atcggcaaac aacggtcgca cgtaactgtc atcgcctgaa ggggcagccg gacggtgata
     8641 aacggtactc atgctgcagg cagccagctg tcgtcctgcc agatttcttc cagcagagac
     8701 aggatacgtt ctttatcctt gtcgttgcgg gctcctgtga cagatagaga cggggcgctg
     8761 ccctggtcta tacgcagcca gatttccggg tagtgtggtt tcagacgttg ttcgatttca
     8821 ttctgtagcg cctgaagtgt gcctgatttg aggttttttg tgctctggcg gtcgaaaaga
     8881 atttcaatgc ggatcatgtt ctcatctccc ttttcttttg ctgtatatgt atacagtatt
     8941 ctaataactg aatccgcaca cagtcaaatg tcattgttct gactaaaaag cgatcaattc
     9001 tctgaggggg tggctgtatg cctgctgtgg agtgctgtat ccagcttatc cacagcattt
     9061 tgtgcacggt tctgtggaca aaatacctgg ttacccaggc cgtgccggca cgttaaccgg
     9121 gctgcatccg atgcaagtgt gtcgctgtcg ccggcctcct cacccggtca cgtttcgtcg
     9181 ttcctcctcc acgcgctgcg gcttcggggc cgcacctgca ttcgtatgcg gtcgcccggt
     9241 tacaggtgcg gcacggcctg atggaggccg catgtgagag gagaattcct atgccaaact
     9301 ggtgctcgaa tcgtatgtat ttttctgccg aaccggcaca gattactgag attaaacgac
     9361 tggccagcgg tgcagtcaca ccgctttatc gccgcgccac aaatgaaggt attcagctgt
     9421 ttctggccgg aagtgccgga cttctgcaga ccactgaaga tgtgcagttt gaaccgtgcc
     9481 ccggactgac ggctgccgga cgtggtgttg tatcgccgga gaatatcgcg ttcacccgct
     9541 ggctgacaca cctgcaggac ggtgtactgc tggatgagca aaactgcctg atgctgcatg
     9601 aactctggct gcagagcggt actggacagc gtcgctggga aggattaccg gacgatgtca
     9661 gggataccat caccgcactt ttcaccgcaa aaagaggtga ctggtgtggc ttctggagta
     9721 acgaggatgt atcggtgtgg tggaaccgtc tgtgtgacaa cgtactgccg gaaaaaacca
     9781 tgccgtttga cctgctgacg gttctgccga cccgcctgga tgttgaagtg aatggcttta
     9841 acggtggtgt tctgaacggt gttccttctg catatcactg gtatacggaa cagtatggcg
     9901 tgaagtggcc ttgtgggtat gaggtgaata tcagcagtca gggagacaac ttcattaagg
     9961 tggatttcga cacgccgtgg tgtcagccgg aaagcgacgt tattgcagaa ttaagccgcc
    10021 gtttcagctg cacgctggag cactggtatg ccgaacaggg ctgtgatttc tgtggctggc
    10081 agttgtatga gcgcggagag ctcgttgatg tgctgtgggg ggaactggaa tggtcttccc
    10141 cgacagatga cgatgagctg ccggaagtca ccggacctgc gtggatagtc gacaaggtgg
    10201 cgcattatgg cggatgaagt atgacggaga cgggcgggca accgcccgtt tcttttccgg
    10261 taaaggatgt cgccgtgctc ctctttttac tgcgccggct gacgcggcgc ggcataaacg
    10321 ctgcctgtgg tcagtgtccc gcgtccggcg ggcacgggac gggcgctttt accgctcccg
    10381 gctggcgtcg gtgacagtgt acgccagccc gtcgcccttt tctgatgaac gcccttcaag
    10441 ccgctttcgc ggcataacct tgccgtcaaa aagacgacgg ctgcggtatt ccacggtcgg
    10501 cctgacccgt taccggacgc ggtgaacagc ccacaggcag cggggaacgg gcaccacagg
    10561 ggtgccctcc cggagccctc tgtaagagaa ggagttttgt atgtcccgtt ttgtccttgg
    10621 taactgcatt gatgttatgg cccgtattcc tgataacgcc attgatttca tgtcgggtag
    10681 cagctgcaca ttactgtgca gccgccccct aagaaccgta cgtgccagtt tcccggcata
    10741 cggctcaagc ctttcaataa ctccacatta gagccggctt cataacagat attccctgat
    10801 gttttgcagt gtgatgacat gccggatgta acatggccag attacctatg ttatccagtc
    10861 ccccttcgga tttcggcaca atgtgtcgaa gttgccagcc tgttgtggcg ttcactggca
    10921 ggtggcagac ggggcactga cctccctgtc ggagccacag atttcttagg cgagagtaac
    10981 cctgtatgga ttccagcatc gtagctttta gccgtgcttc aaagtacgat tcgtagctgg
    11041 gatcaaaagg gttagcagtg ctttttattt gtctgtgtct tttgactggc atatccgaga
    11101 tttcatatag cgccagtaac tcacgtttgc ctgatggcag gagcttccct gtttctgtgc
    11161 cgaataccca gtgtcggaag ttaactgaac gaaagtattt ctttcttatc cagcctgtac
    11221 ctttctttgg gtgtcgtcga catgcccagc gccatagtgc tttccatatt tcatgactga
    11281 ctttctgaaa gatctttttt gaaacaacat gacgatagta tctgacccag cctttgagga
    11341 ctgggttgag atgccatatc agtgtgctct ggctgactgt cggattgtct tttatcgtct
    11401 ggcggaccct tttcaggaat tcctttacct tttggggtga aggtttaatc aacagtttcc
    11461 cgttatattt gcggatgttc atacccagaa agtcgaatcc atcactgatg tgtgtgattc
    11521 gtgtcttttc tggtgagagg gtaagccctc tttctgccag aaagttttct aacaagggaa
    11581 gaaccttttc tgacagcgtg tcgtggctta tccccgtgat cacaaaatcg tccgcataac
    11641 ggattatgtt gatcttttct ggattcagtc cgcctcgata gaaggttttc ttcaacagag
    11701 gctgcagacc atccagaacc atgtttgcca gcgttggaga tattattccg ccctgtggag
    11761 ttcctgcact ggttgggtat aatttcccca aatcaatata tccggctttc agccatcgtt
    11821 tcaggatcgc cgtatccgtt gggatattgg cgattagcca ttcatgtgaa atctcatcaa
    11881 aacaactgcg aatgtcggct tccagaatcc attgtgcccg atcagccctg tgcaacgttg
    11941 tgaaacattg ctcaatggca tcggctgttg agcgacaggg cctgaaccca aacgaatttg
    12001 ggtcagccgt tgtttctgca acaggttcca gcgccatcag atagagtgcc tgcatggctc
    12061 tgtctttcat tgtcgggatc cctaatggtc gctttttgcc gttagatttt gggatatata
    12121 tacgccgtag tggaagaggt ttgtaacccc ttcttttcag cgtgtatata gccttcatct
    12181 tgctgccggg ttttgaccat gttttgccat caacacccgg cgttctcttc cctctgttat
    12241 ctgtcactcg tttaactgcc attgctctgg cgctgaacga gtgggtgagt agccattgca
    12301 aggcttttac cttgcccgct ttctcctcct gtattgcctt cacgatacgc gcctgcagcc
    12361 ttctgacctc gcgacggcac ttggcccagt tgatattgtg ccagtgagtc acgatgtcgg
    12421 tgggtgcacc cgcgtttatg tttttcgcag tcatctgctt accctccttc aaaggtattc
    12481 aggttatctc gtgttgaaag accatgcgga agtctgcccg ctttcgcgtg gaatgatgtt
    12541 gctgaaacag ctcaactcct gtccatccca ttacaggatg gcattcgctt tctccgcact
    12601 cctgtacccg catcactgac agctttcctc gcggttagcc tgccctgagg gcagtgatac
    12661 gggcttaccg tgttccgcac agtttgcagg gatgggttag gttctgccta tccaccggta
    12721 gctttgatgt ccgtgcctcc ccagtcaaaa gaggaaggtc cagctacgtt cccattttgg
    12781 gtcgggcctg tcagtgcctt tggcccgttc agggttgacg atgtttatca gcagttcaca
    12841 tgtgttaacc ctgccatcca gccttgcgcc ccatccgccc tgacactggc agacatgctt
    12901 cctcctcgcg gattccacat ggtgtgaaaa ccgggctacg ttgtcccgaa ggcttcatac
    12961 cgaacagtta cccgctctgc atgcttcggt aggctacagg cgacggaacg cctggtttta
    13021 ctacttcctt tacatgaagt taaaacaaca aactgtgcga ctttcacgtc gcacccttac
    13081 cgatcctcca tatctcgtcg gtttccgtga ccgtcagggg cgcaccattg ccggtgacgt
    13141 taatgatgac tggctgcaac ctgccagcaa tgaaatgtac cgcgtactca aaaaagatgc
    13201 gctgatggtg agtttctatg gctggaaccg tattgaccgc tttatggcag cctggaaacg
    13261 cgccggattc agcgtggcag ggcaccttgt gttcaccaaa aactacacat ccaaatctgc
    13321 gtatgtcgcg taccgccacg aatgcgctta catcctggca aagggcagtc cggcccggcc
    13381 ccgtaacccg ctgccggatg tgctgggctg gaaatattcg ggcaaccgcc atcacccgac
    13441 ggaaaagccc gttaccagcc tgcaaccact gattgagagc ttcacacatc cgaacgcgat
    13501 tgtgctggac ccgtttgcag gcagcggctc aacctgcgtc gccgccctcc agtccggacg
    13561 caggtatatc ggtatcgagc tgcttgagca gtatcaccgt gccggacagc aacgtctggc
    13621 tgccgtacag cgggccatgc agcagggggc cgcaaatgat gactggttta tgccggaggc
    13681 tgcgtaaatg aactatgccg gacatgaaaa acttcgcgcc gaagtggcgg aggtggccaa
    13741 tgccatgtgc gacctgcgta caaccatgaa tgagatggag cggcggtaca gctttaatgc
    13801 tgacaccctg ccggaacgtc tggtgcgtca gacgttgttt cgcgcaaacc gcctcctgat
    13861 ggaggcatat accgaaattc tggaactgga ttcctgcttc aaagattgag gggagagagt
    13921 atgtacggaa catgcgaaat actctgccgg gagctggcag caaagtatcc gggagacacg
    13981 ccgctgatgc tggttatctg gtctccggaa gagattcagg cccttgctga cggaatggat
    14041 atttccctgt ccgatcatga aatcaggacc gtcctggcgc gcctggagga catcccggaa
    14101 gaccagcgga ctgagtccgg tatttcttcg ggtgcggcta tggagattat cagcaacgtg
    14161 agtgaaaacc gccaggtgac cgtccctgct gaactgctgg cgtccctgat tcagaccgct
    14221 gaacaggcat tgtggaagcg tgaatgggcc gcccgtgata acggcctcgt cgtcccggaa
    14281 tgcgtcaccc gccgtcaggc ggtggttaat caggcccgcg ccctgctgaa aaacaacaca
    14341 cacgaaaacg actgatgtta tcgccgcttt cgggcggcga ttcagggaga ccattaatca
    14401 tgaacaaaac gctgaatgca ctggtttgtc gccacgcccg taacctgctg ctggcgcagg
    14461 gctggccgga agagacggat attgaacaac tgaattcgca ctatccgggc tggatcagca
    14521 tttatgtgcg actggatgta tcccggctgg cgacgttact tatcagccgc catgatggcg
    14581 tgctgccgcc ggtcctggcg tctgccatgc aaaaactgac cggaaccgtg gcggaacttg
    14641 tactgtccgg cagccagtgg caggcgctgc cggtacttcc ggcagacgga acgcaggtgt
    14701 ctttcccgta tgccggtgaa tggctgacgg aggaagaaat ccgggcagtt cttgatgcgg
    14761 tgcgcaatgc agtacgaagc atctgttacc aggtggcaga cgatgcgcgg cgtatccggg
    14821 cggcgctgac caccaccggt cagacgttgc tgacccgcca gacgcgccgc tttcgtctgg
    14881 ttgtgaagga aagcgattac ccctgctggc ttgatgaaga tgacgaaaat ctgcccgtgg
    14941 tgctcgatgc cattctgaac cggggcgccc gtttttcctc tgtggaaatg tacctggtct
    15001 gtgagtgtgt ggagcatatc ctggccagtg gtctggtctg tgatgtgctg cgtataccgg
    15061 atgaaccgtc ccgtcgctgg tttgacaggg atattctgcg ggaggttgtt ctggaggcac
    15121 gggatgaaat ccgcagcatg gcagatgcac tggcgaaaat ccggaaatga tgacgctggc
    15181 ggggaaaaat ctccgccttt tcctgccggt ccccggacag agcaaaagga tgtcgccgca
    15241 ggctccgctt tacccggcca tgcggggcat ggccttgtgg gttttcagtt atgtggcctc
    15301 agcgtcgtgt gcgggctgtg ccgtgcctcc atcttagccg ggctggcggg gatgcaaggg
    15361 taaaatgcac gcggcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt
    15421 tcacccgctc agatttcgcg gtctcaccct ccaactcccg tcagccgtgt gcgtcaggct
    15481 gcggcttccc ttgcgccctt gcatccccgc cttatcgccc ggcttttctg gaggcacggc
    15541 accgcccggt gtcgcggaat atgtgaacaa cggaggaaat cccatgcagt atgcgaaacc
    15601 tgtcactctg aacgttgaag agtgcgaccg cctttccttt ctgccttacc tgtttggcaa
    15661 cgattttctg tatgccgagg cgtatgtgta cgcgctggcg caaaaaatga tgccggaata
    15721 tcagggaggc ttctggcatt tcatccgcct gccggatggt ggcggttaca tgatgccgga
    15781 tggcgaccgt tttcatctgg tcaacggtga aaactggttt gaccgcactg tgagtgctga
    15841 tgccgcaggt atcatcctga cttcccttgt gattaaccgc cagttgtggc tgtaccacga
    15901 cagcggggat gcgggactga cacaccttta ccggatgcgt gatgcgcagt tgtggagcca
    15961 cattgaattt catcccgaat gtaacgcgat ttacgcagca ctggactgat taacggacgg
    16021 ggcggcgatg ccgcccctgt aaaggagacg agaacatgta ctgtactgtt aaagaaatta
    16081 tccgcgaagt gctgaataca gacgtgccgg acagtgaatg tgtttttgcc gtggtgctga
    16141 cccgcgggga tgtgcgccac atagcccggg actggaacct gtcagacgat gagctggaaa
    16201 ccgtcataca gcggctggac gatgcttttg tatatggcgc atgtgacaga gttgtttccg
    16261 atattgtcaa tgaactgatg gaggaaaagc gcgtaaaccg tcttgtaaca gtccctgcgg
    16321 tgctgctgga aaaagtgatg gtgatggctg gtagtgaaat ttatcgcctc catgctgtag
    16381 ggagcgagaa cgggggcgac ggtgacgcat ttgtcaggga agaacgtgag attatgcggg
    16441 taatgcgtca ggcgctggac ggggagaacg ggtaatgaat atcagcacgg aaacccgcga
    16501 aattctgcgt aattacaaag ctgtgattaa tgcgcggcgt cgtgaaatgg ggcagaaacc
    16561 gctcaccacc gcgcagattg ttgatgaaat ctgtgatttt gtggcgaatc agcaggcggt
    16621 tttcctcggt ggtcactata tccttcaggg cagcagaaac aggtgataca gggcaggcgg
    16681 tggaggccgc ctgtcagccc gcgcgaaaac ccctggcagg ctggcgcctg ccggaaggga
    16741 agcgggccac gctggcgcgt cgcccgttac ccttcctgtt ggtgacaggc gttgtttcag
    16801 gaagtacctg ttacagacct gtcgttaaag aaccttctta cacatgcgcc ccttcggctc
    16861 ccggctgcct ggcggcatcc ggtcgtcggc ccgactacga caggcagctg ctggcgcacc
    16921 cgccagtctc cgccgaaccc gctggcgcgg tttcgggctg tcgctacggt ccgatgcgtg
    16981 aaaacccggg cttgcagcga ccggaacggt cgcgccgcct ttgtcgcgcc gtggcctgcg
    17041 ctcctggcat cccggcaaaa ggccgccggg atgtaaggaa accgcgcctg atggcgcggc
    17101 agtctcctct tctgccgcca cctctgcggc ctgtttcccg ctgtggactt tccggggggc
    17161 agacgtaacc acctcccgtt ccatcagtcc aggggaagat ataccggcca caaaattttt
    17221 agccatgaac ccggcaaaaa attttgcgtc cgcccccgga cagctgtccc gtgaggcggt
    17281 aaacggg
//
LOCUS       DAZKJR010000111        17160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKJR010000111 DAZKJR010000000
VERSION     DAZKJR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.112"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            445..1830
                     /gene="dcuC"
                     /locus_tag="ACHOWS_003703"
     CDS             445..1830
                     /gene="dcuC"
                     /locus_tag="ACHOWS_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFQ0049217.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1871..2551)
                     /gene="dpiA"
                     /locus_tag="ACHOWS_003704"
                     /gene_synonym="citB"
     CDS             complement(1871..2551)
                     /gene="dpiA"
                     /locus_tag="ACHOWS_003704"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFQ0049218.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2520..4178)
                     /gene="dpiB"
                     /locus_tag="ACHOWS_003705"
     CDS             complement(2520..4178)
                     /gene="dpiB"
                     /locus_tag="ACHOWS_003705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFQ0049219.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIHSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4558..5616
                     /gene="citC"
                     /locus_tag="ACHOWS_003706"
     CDS             4558..5616
                     /gene="citC"
                     /locus_tag="ACHOWS_003706"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFQ0049220.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFCHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5631..5927
                     /gene="citD"
                     /locus_tag="ACHOWS_003707"
     CDS             5631..5927
                     /gene="citD"
                     /locus_tag="ACHOWS_003707"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFQ0049221.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5924..6832
                     /gene="citE"
                     /locus_tag="ACHOWS_003708"
     CDS             5924..6832
                     /gene="citE"
                     /locus_tag="ACHOWS_003708"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFQ0049222.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6843..8375
                     /gene="citF"
                     /locus_tag="ACHOWS_003709"
     CDS             6843..8375
                     /gene="citF"
                     /locus_tag="ACHOWS_003709"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFQ0049223.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVYLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8379..8930
                     /gene="citX"
                     /locus_tag="ACHOWS_003710"
     CDS             8379..8930
                     /gene="citX"
                     /locus_tag="ACHOWS_003710"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFQ0049224.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKLGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8905..9783
                     /gene="citG"
                     /locus_tag="ACHOWS_003711"
     CDS             8905..9783
                     /gene="citG"
                     /locus_tag="ACHOWS_003711"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFQ0049225.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9834..11297
                     /gene="citT"
                     /locus_tag="ACHOWS_003712"
     CDS             9834..11297
                     /gene="citT"
                     /locus_tag="ACHOWS_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFQ0049226.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11411..12217
                     /gene="rna"
                     /locus_tag="ACHOWS_003713"
     CDS             11411..12217
                     /gene="rna"
                     /locus_tag="ACHOWS_003713"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFQ0049227.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETATKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISVKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12447..12857
                     /gene="rnk"
                     /locus_tag="ACHOWS_003714"
     CDS             12447..12857
                     /gene="rnk"
                     /locus_tag="ACHOWS_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFQ0049228.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            13000..13089
                     /gene="yldA"
                     /locus_tag="ACHOWS_003715"
     CDS             13000..13089
                     /gene="yldA"
                     /locus_tag="ACHOWS_003715"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFQ0049229.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            13214..13642
                     /gene="uspG"
                     /locus_tag="ACHOWS_003716"
     CDS             13214..13642
                     /gene="uspG"
                     /locus_tag="ACHOWS_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFQ0049230.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(13763..15328)
                     /gene="ahpF"
                     /locus_tag="ACHOWS_003717"
     CDS             complement(13763..15328)
                     /gene="ahpF"
                     /locus_tag="ACHOWS_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0049231.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(15499..16062)
                     /gene="ahpC"
                     /locus_tag="ACHOWS_003718"
     CDS             complement(15499..16062)
                     /gene="ahpC"
                     /locus_tag="ACHOWS_003718"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0049232.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            16434..>17160
                     /gene="dsbG"
                     /locus_tag="ACHOWS_003719"
     CDS             16434..>17160
                     /gene="dsbG"
                     /locus_tag="ACHOWS_003719"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFQ0049233.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKMLN"
BASE COUNT         4100 a         4459 c         4232 g         4369 t
ORIGIN      
        1 tcgattaaag atgatgcgtc gttttctgcg atgggaatag tcaaaaaaga aaaatcaagt
       61 cttttttgat gacaaaatgc aatcaaggaa aaattatttt attttttaac gagatacaac
      121 aatcatctta acgaagtata taatatatat cgtcaaccta tgctcatatt agattatttt
      181 ttgagcgcac agccgcagct aacaccattg caattaacaa atttgcatca atccaccatc
      241 aatttacaca cattattatg tgataattgc caaccgctaa atatgcgttt tgttatctat
      301 gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta atcagcattc
      361 atcgccaatt tattgggcat attttttcct taagctttag gaatttttta ttatttactt
      421 tggggcctgg agacaggaaa aattatgctg acattcattg aactccttat tggggttgtg
      481 gttattgtgg gtgtagctcg ctacatcatt aaagggtatt ccgccactgg cgtgttattt
      541 gtcggtggcc tgttattgct gattatcagt gccattatgg ggcacaaagt gttaccgtcc
      601 agccaggctt caacaggcta cagcgccacg gatatcgttg aatacgttaa aatattgcta
      661 atgagccgcg gcggcgacct cggcatgatg attatgatgc tgtgtggctt tgccgcttac
      721 atgacccata tcggcgcgaa tgatatggtg gtcaagttgg cgtcaaaacc attgcagtat
      781 attaactccc cttacctgct gatgattgcc gcctattttg tcgcctgtct gatgtctcta
      841 gccgtctctt ccgcgaccgg tctgggtgtt ttgctgatgg caaccctgtt tccggtgatg
      901 gtaaacgttg gtatcagccg tggtgcagct gctgctattt gtgcctcccc ggcggcgatt
      961 attctcgcac cgacttcagg ggatgtggtg ctggcggcgc aggcttccga aatgtcgctg
     1021 attgacttcg ccttcaaaac aacgctgcct atctcaattg ctgcaattat cggcatggca
     1081 atcgcccact tcttctggca acgttatctg gataaaaaag aacacatctc tcatgaaatg
     1141 ttagatgtca gtgaaatcac caccactgcc cctgcgtttt atgccatttt gccgttcacg
     1201 ccgatcatcg gagtgctgat ttttgacggc aaatggggtc cgcaattaca catcatcact
     1261 attctggtga tttgtatgct gattgcctcc attctggagt tcatccgcag ctttaatacc
     1321 cagaaagttt tctctggtct ggaagtggct tatcgcggga tggcagatgc atttgcaaac
     1381 gtggtgatgc tgctggttgc cgctggggta ttcgctcagg ggcttagcac catcggcttt
     1441 attcaaagtc tgatttctat cgctacctcg tttggttcgg cgagcatcat cctgatgctg
     1501 gtattggtga tcctgacaat gctggcggca gtcacgaccg gttcaggcaa tgcgccgttt
     1561 tatgcgtttg ttgagatgat cccgaaactg gcgcactctt ccggcattaa cccggcgtat
     1621 ttgactatcc caatgctgca ggcgtcaaac ctcggccgta ccctgtcacc cgtttctggc
     1681 gtagtcgttg cggttgccgg gatggcgaaa atctcaccat ttgaagtcgt aaaacgcacc
     1741 tcggtgccgg tgcttgttgg gctggtgatt gttatcgttg ctacagagct gatggtgcca
     1801 ggaacggcag ccgcggtcac aggcaagtaa atagtcatgc cggaggcaag tttcctccgg
     1861 ctttatcatg tcacccactg tggtatatgc gttgtggtct gccaactttg ccgtgaacaa
     1921 tttcagcaat aatcagatgg cggctggcgc aatattcaag ataacgtctg gcagtggtgc
     1981 ggctgatagt cagtgcctgc gccaccgttt ctgccgtatg ttgcacacca ggctctttaa
     2041 acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc ggtcggcagt tcgtctttgg
     2101 gttcaccgcg agcataggca ttaaacatct catcgatttg cttctggctg gcgctatcaa
     2161 tactttccag catatgttta cgctggcgga aacgggttag cgtttgcccc agccgttcat
     2221 aggcaattgg cttaatgaga taatcaaata caccacaacg tacggcttca gacaccgttt
     2281 ccatatcact ggctgcagtg gtaaacacca cgtcgccggg ataatgcgcc tgcaccagtt
     2341 catgcagtaa attaatccct ctaccgtcag gaagatagtt atcgagcaag attagccccg
     2401 gcttaaaacg ctcgatcatc attcgggcct gcgccaggtt tcccgccagt aatatctgac
     2461 tgaatccggg aatgtgacga atatattccg catgcatctc tgccagcggc gtttcgtcct
     2521 caacgatcaa tagggttaat ggagctgtca ttaggtttca ctttcggaat atagattgaa
     2581 aataaggtac cgcagggatc attatcttcg agagtgataa caccaccgca gcgcgttacg
     2641 tagctggcaa tcaagtacaa cccaatgcca tgttcgccgg gctcgtcagc acgcgtactg
     2701 acaccctgct caaatatttt gtctcgtaga gactctggaa cgccgcagcc ctgatcggcg
     2761 acttcaatca ccacatcatc gccttcatcg ctgaggaata attcaacgat cttgtttcct
     2821 tcatcgctac gcaggctggc ttcgaaggcg ttatcaagta aattgccaac aatggctgca
     2881 aactcggtgc tgtccagtcc tggcggcagt tgcgaaagct gacttccggg gacgatgacc
     2941 atttttagcc ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc cgccacctgg
     3001 cgatcggcaa acgcctcgcg caggctatca ataagctgtt gctgggcctg agactccccc
     3061 tgcaccatcg ccaacacgcg atcatactct ttcatctgca acagaccatt gagcgtcgac
     3121 atccaattga gatgctcgtg tcgcaatgtg cgcaggctct cgacgtattg tttaatttgc
     3181 gtcagttgtg cattgagggt ggatatttcg tctttactac gaaagctgat aatggccccc
     3241 agcaaatcat caccagaatg aatagcttcc cggttggcaa taacgctcag accgttaaag
     3301 ttcgccacca catcctgacg tttttcatca atctgttcgg taaagaaatc ggcgggcctg
     3361 accacttcag caatgggttt acccaaccat tgccgtccgg gggagctcag ccccagcatc
     3421 tttcttgcgt tacgattgat ggcggtaatg taaccgtgcg gatccaccgc aatgagccct
     3481 tcataaaccg aactaaacag cgcctcttgc tgacggacca cgcgtgcgat ttgctttggc
     3541 tccatgccca tcatctgccg acggatatgc gcggccagga accacgacag caacatcaga
     3601 atccctaaca gcacgacaaa cacgccagcc atcggtaata aaaactcagc ccgccagcta
     3661 tcgattttac tcaccaggta gccaatcgac accacgccga tgacttttcc atcgtcatca
     3721 aagattggcg ttttggcacg catcgccatg ccaattgacc ctttcccggt gatgaagtag
     3781 ctctcccctt tctccagcgc gcccggtttg gtgaactgca taggataacc aattttctcc
     3841 ggattaggat ggtaaagacg aatcgagtgc cgatcgccaa tcaccacata atcgaaatca
     3901 gtatctcttt gtaatttatc agcgatggtc gccagcctct tgtagtcacg cgttttcact
     3961 gcgctgatga tactgtcatt agaagcaata atttttgcct gattcattgc catatcgcgc
     4021 acatgaagcg ttaaatagtc ctcaaaactg gccgtaaaat attgcgccag ggctgcaatg
     4081 acaaatattg agaacaccag aatcagcaaa aagatacgca gcggaaatgc cagtctttgg
     4141 aaaaatgcaa actgtttatt ctcgttaagc tgcaacattg ttttccttgc gggttgtccc
     4201 gagcctcttt gacgaatgta attaaaatgt tgaatcaaaa tacatagata ttaaatataa
     4261 tcgtggcaaa ttttattcat taaatcaatt aaatcactta aacctgcggg tcatttcgta
     4321 attacattaa ttaaaaaact taaaaccatt aaataaataa aaaaccacta actctatgtg
     4381 aaataaatca aaatttcacg ctgcaatact ccttaggatg tatagcgaaa ggagaaaaag
     4441 atatacctcg atcgcccccc tttctcccaa gtgaaaataa aaggttatca gtttgcaaca
     4501 tcgaacaaca tttgttgcaa atcgataaca acatgcacct tcaggatact atttattatg
     4561 ttcggcaatg atattttcac ccgtgtaaaa cgttcagaaa ataaaaaaat ggcggaaatc
     4621 gcccaattcc tgcatgaaaa tgatttgagc gttgacacca cagtcgaagt atttattacc
     4681 gtaacccgcg atgaaaagct tatcgcgtgc ggtggaattg ccggaaatat tattaaatgc
     4741 gttgctatca gtgaatccgt tcgcggtgaa ggactggcgc tgacattagc caccgagcta
     4801 attaacctcg cctatgagcg gcacagcacg catctattta tttataccaa aaccgaatac
     4861 gaggcgctgt tccgccagtg cggtttttcc acgctgacca gcgtacccgg cgtgatggtg
     4921 ctgatggaaa acagcgccac gcgactgaaa cgctatgccg aatcgctgaa aaaattttgt
     4981 catccaggga acaagattgg ctgcattgtg atgaacgcca atccctttac gaatggtcac
     5041 cgttatctga ttcaacaggc tgcggcacag tgcgactggt tgcatctgtt tttagtcaaa
     5101 gaagattctt cacgcttccc ctatgaagac cgactggatc tggtgttaaa aggcaccgcc
     5161 gatattccac gcctgactgt gcatcgcggc tccgaataca tcatctcccg cgctacgttc
     5221 ccttgctact tcattaaaga acagagcgtc attaaccatt gttacaccga aattgatctg
     5281 aagattttcc gtcagtacct cgctcccgcg ctgggtgtaa ctcaccgctt tgtcggtact
     5341 gaaccctttt gtcgcgttac tgcccagtac aaccaggata tgcgctactg gctggaaaca
     5401 ccgactatct ccgcaccgcc catcgaactg gttgaaattg agcggctgcg ttaccaggag
     5461 atgccgatat ccgcttcccg ggtacgtcaa ctgctggcga aaaacgatct cacggctatc
     5521 gcgccgctgg tccctgcagt cacgctgcat tatttgcaga acctgcttga gcactcccgc
     5581 caggacgcgg cagctcgtca aaagaccccc gcatgagaaa caggtgaaaa atgaaaataa
     5641 accagcccgc cgttgcaggc acccttgagt ctggggatgt gatgatacgc atcgccccac
     5701 tcgatacgca ggatatcgac ctgcaaatca acagcagcgt tgagaaacag tttggcgatg
     5761 caattcgcac caccattctg gacgttctcg cccgctacaa cgtgcgcggc gtacagctga
     5821 atgtcgatga caaaggcgca ctggactgca ttttacgtgc acgactggaa gccctgctgg
     5881 cacgcgccag cggtatcccg gctctgccat gggaggattg ccaatgattt ccgcttcgct
     5941 gcaacaacgt aaaactcgca cccgccgcag tatgttgttt gtgcctggtg ctaatgccgc
     6001 gatggtcagc aactccttta tctacccggc tgatgccctg atgtttgacc tcgaagactc
     6061 cgtagcattg cgtgaaaaag acaccgcccg ccgcatggtt taccacgcgc tgcaacatcc
     6121 gctttatcgc gatattgaaa ccattgtgcg tgtaaacgcg ctggattccg aatggggtgt
     6181 taacgacctg gaagctgtcg ttcgcggtgg tgcggacgtt gtgcgtctgc cgaaaaccga
     6241 taccgctcag gatgttctgg atatcgaaaa agagatcctg cgtatcgaaa aagcctgtgg
     6301 tcgtgaaccc ggcagtaccg gcctgctggc ggcgattgaa tctccgctgg gcattacccg
     6361 cgcggtagaa atcgctcacg cttccgagcg tttgatcggt atcgccctcg gtgcagaaga
     6421 ctatgtgcgc aacctgcgta cagaacgctc cccggaagga actgaactgc tgttcgctcg
     6481 ctgctccatt ttgcaggccg cgcgctctgc gggtattcag gcgttcgata ccgtctattc
     6541 cgacgctaac aacgaagccg gattcctgca agaagccgcc cacatcaaac agctgggctt
     6601 tgacggcaaa tcgctgatca acccgcgtca gattgatctg ctgcacaacc tctacgcacc
     6661 gacccagaaa gaagtggatc acgcccgccg cgtcgtagaa gccgctgaag ccgccgctcg
     6721 cgaaggcctc ggcgtggttt ccctgaacgg caagatggtg gacggtccgg ttatcgatcg
     6781 cgcccgtctg gtgctctccc gtgcagaact ttccggcatc cgcgaagaat aaggcaatca
     6841 aaatgacgca gaaaattgaa caatctcaac gacaagaacg ggtagcggcc tggaatcgtc
     6901 gcgctgaatg cgatcttgcc gctttccaga actcgccaaa gcaaacctac caggctgaaa
     6961 aagcgcgcga tcgcaaactg tgcgccaacc tggaagaagc gattcgtcgc tctggtttac
     7021 aggacggcat gacggtttcc ttccatcacg ctttccgtgg cggtgacctg accgtcaata
     7081 tggtgatgga cgtcatcgcg aagatgggct ttaaaaacct gactctggcc tccagctccc
     7141 tgagtgattg ccatgcgccg ctggtagaac acattcgcca gggcgtggtt acccgcattt
     7201 atacctctgg cctgcgcggt ccactggcgg aagagatctc ccgtggtctg ctggcggaac
     7261 cggtgcaaat ccactctcac ggcggacgtg tgcatctggt acagagcggc gaactgaata
     7321 tcgatgtggc tttcctcggc gtcccgtcct gtgatgaatt cggtaatgcc aacggctact
     7381 ccggtaaagc ctgctgcggc tccctcggct atgcaatggt tgatgccgac aacgcaaaac
     7441 aggtcgtgat gcttaccgaa gaactgctgc cttatccgca taatccggca agcattgagc
     7501 aagatcaggt tgatctgatc gtcaaagttg atcgcgttgg cgatgctgca aaaatcggcg
     7561 ctggtgctac ccgtatgacc actaacccgc gcgaactgct tattgcccgt agcgctgcgg
     7621 atgtgattgt taactctggc tacttcaaag aaggtttctc catgcagacc ggcaccggcg
     7681 gcgcttcgct ggcggtaacc cgtttcctgg aagacaaaat gcgtagccgc gatattcgcg
     7741 ccgacttcgc cctcggcggt attaccgcaa cgatggttta tctgcacgaa aaaggtttga
     7801 tccgcaaact gctggatgtg cagagctttg acagccatgc tgcgcaatcg ctggcccgta
     7861 accccaatca catcgaaatc agcgccaacc agtacgctaa ctgggggtcg aaaggcgcag
     7921 cggttgatcg tctcgacgtg gtggtactga gcgcgctgga aattgacacc cagttcaacg
     7981 ttaacgtgct gaccggctct gacggcgtac tgcgtggtgc ttccggtggt cactgcgata
     8041 ccgcgattgc ttctgcgctt tccatcatcg tcgcgccgct ggtacgcggt cgtattccga
     8101 cgctggtgga taacgtgctg acctgcatca ccccgggctc cagtgtcgat attctggtca
     8161 cagaccacgg catcgctgtt aacccggcac gtccggagct ggcagaacgt ctgcaggaag
     8221 cgggcattaa agtggtttcc attgagtggc tgcgtgaacg tgcgcgtctg ctgaccggtg
     8281 aaccacagcc gattgaattc accgaccgcg tcgttgccgt tgtgcgttac cgcgatggct
     8341 cggtgatcga tgttgtgcat caggtgaagg aataagccat gcacctgctt cctgaactcg
     8401 ccagccacca tgcggtgtca attcccgagc tgctcgtcag ccgggatgaa aggcaagcac
     8461 ggcaacacgt ctggctcaag cgccatcctg ttccactggt ctcctttacc gtggttgcgc
     8521 ctgggccgat taaagacagc gaggtcacac gccgaatttt taatcatggc gtgacagcct
     8581 tgcgtgcctt agccgcaaaa ctgggctggc aaattcagga gcaggctgca ctggtttccg
     8641 ccagcgggcc ggagggcatg ttgagcattg ccgccccggc tcgcgacctc aagctcgcca
     8701 ccattgagct tgaacatagt catcctctcg ggcggttatg ggatatcgat gtcctgacac
     8761 ccgaaggcga aattctctcc cgccgcgact attcactgcc gcctcgccgc tgcctgttgt
     8821 gcgagcaaag cgcagctgtt tgcgcgcgtg gaaaaaccca tcaactgacc gatttactca
     8881 accgcatgga ggcgctgctg aacgatgtcg atgcctgcaa cgtcaactaa aaacacaaag
     8941 cttgcgacgt cattaatcga tgagtacgcc ctgctgggct ggcgcgccat gctgactgaa
     9001 gtcaatctgt caccgaaacc aggcctcgtg gatcgcatta actgcggtgc gcacaaagat
     9061 atggcgctgg aagatttcca ccgcagcgcg ctggcgattc agggctggtt accccgtttc
     9121 attgaatttg gtgcctgtag tgcagaaatg gcaccggaag cggtactcaa cggattacgc
     9181 ccaattggta tggcttgcga aggtgatatg ttccgcgcca ctgcgggcgt aaacacgcat
     9241 aaaggaagca ttttttcttt agggctactg tgtgcggcaa ttggtcgttt gcttcagctc
     9301 aaccagccgg taacgccaac aaccgtttgt tctacggcgg caagtttctg ccgtggcctg
     9361 accgatcgcg aactgcgtac caataattca cgactgacgg caggtcaacg gttgtaccag
     9421 cagcttggcc ttaccggcgc acgcggtgaa gccgaagcgg gttatccact ggtgatcaac
     9481 cacgccttgc cgcattacct cactctgctg gatcaggggt tagatcctga gctggcattg
     9541 ctcgatacct tgctcctact gatggctacc aacggcgata ccaacgttgc atcgcgcggt
     9601 ggcgaggggg gcctgcgctg gctacagcgc gaggcgcaaa cattattgca aaaagggggc
     9661 attcgaaccc ccgccgatct cgattatctc cggcagttcg acagggagtg tatcgaacga
     9721 aatctcagtc caggcggcag tgccgacctg ctgatcctta cctggttttt agcacagatt
     9781 taattattta agcacttgat aaatttggaa atattaattt tcggagaacc cgtatgtctt
     9841 tagcaaaaga taatatatgg aaattattgg cgcccctggt ggtgatgggt gtcatgtttc
     9901 ttatccctgt acctgacggt atgccgccgc aggcgtggca ttactttgca gtgtttgtgg
     9961 caatgattgt cggcatgatc ctcgagccaa ttccggcaac ggcgatcagt tttattgcgg
    10021 ttactatttg cgttattggc agtaattacc tgctctttga tgccaaagaa ttagctgacc
    10081 cagcgtttaa tgcgcaaaaa caggcgctga aatggggtct ggctggtttt tccagcacca
    10141 cggtatggct ggtatttggc gcatttattt ttgcattagg gtatgaagtt tccgggttag
    10201 gccgtcgtat tgcccttttc ctggtgaaat tcatgggcaa acgcacgctg acgttgggtt
    10261 atgcgattgt cattatcgac attctgctgg caccgtttac accgtccaac accgcgcgta
    10321 ccgggggtac ggtttttccg gtcattaaaa acctgccgcc gttgtttaaa tcattcccga
    10381 acgatccgtc cgcgcgtcgt attggcggct atttgatgtg gatgatggtc attagtacca
    10441 gtctgagttc gtccatgttt gtcaccggtg cggcaccaaa cgtgctgggt ctggagttcg
    10501 tcagcaaaat tgccggtatc cagattagct ggttgcagtg gttcctctgc ttcctgccgg
    10561 ttggggttat cttgcttatc attgcgccgt ggctttccta cgtgctgtac aaaccggaaa
    10621 tcacacacag tgaagaagtg gcaacctggg cgggtgatga actaaaaacc atgggtgcgc
    10681 tgacacgcag agagtggacg ctgattggcc ttgtattgct cagcttaggt ttgtgggtat
    10741 ttggcagtga agtcattaat gctactgcgg ttggtctgct ggcagtttcg ctaatgctgg
    10801 ccctgcacgt tgtgccgtgg aaagacatta cccgctataa cagcgcatgg aacacgctgg
    10861 tcaacctggc aactctggtt gtgatggcta acggcctgac tcgttctggt tttattgact
    10921 ggttcgccaa taccatgagt acgcacctgg aaggattctc accaaacgca acggtgattg
    10981 tactggttct ggtgttctac tttgcacact acctgtttgc cagcctgtct gcgcacaccg
    11041 caaccatgct gccggttatt ctggccgtcg gtaaaggtat tccgggcgta ccaatggaac
    11101 aactgtgtat cctgctggtg ctatctatcg gtatcatggg ctgtctgacg ccgtatgcaa
    11161 ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat tactggcgtc
    11221 ttggcgcaat cttcggggtg atttacatct ctatgttgct gttggttggc tggccgattc
    11281 tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc ccgccaggta
    11341 aacccacctg gcgggctttt ttatgattaa atagataata ttcagtgtat gtatgagttc
    11401 cacacccatt atgaaagcat tctggcgtaa cgccgcgttg ctcgcggttt ctctgcttcc
    11461 cttctcttct gccaacgccg tggcgttgca ggcaaaacag tatggcgatt ttgatcgcta
    11521 tgtcctggcc ctctcctggc aaaccggatt ttgccagagt caacacgatc gaaatcgtaa
    11581 cgaacgagat gaatgtcgcc tgcaaaccga aacgaccaac aaagctgatt ttctgaccgt
    11641 acatggtctg tggccaggat tgcctaaatc ggttgctgcc cgtggtgttg atgaacgccg
    11701 ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat ctaccagaag cgcgcgccag
    11761 ccgaatgtgt tcatcgccgg aaaccgggtt atcactggaa acggccacta aactaagtga
    11821 agtcatgcca ggagctggcg gacgttcctg cctggaacgc tacgaatatg ccaaacacgg
    11881 tgcctgcttt ggttttgatc cagacgcata cttcggcacg atggtacgcc tgaatcaaga
    11941 aattaaagaa agcgaagccg gaaaattcct tgcggataac tacggtaaaa cagtgagccg
    12001 ccgtgacttt gatgccgcct ttgccaaaag ctggggaaaa gagaacgtga aagcagttaa
    12061 gctaacgtgc cagggtaacc ctgcgtattt gactgaaatt cagatctcgg tcaaagctga
    12121 cgccatcaac gctccgcttt ctgccaactc attcttgcca caaccacacc caggcaactg
    12181 tggcaaaacc tttgtgattg ataaagcagg ttattaatcc tctgatttac aaaacacgct
    12241 aattcgtctt ccataaacca tgacgattaa gatttataca taatgcgcta cattgaggaa
    12301 ttccctgaat gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat catcatgtga
    12361 gttaaaccct cgccgcctga cggtgagggt tttcttttgg gtatgtttat ctgcgacact
    12421 cgcagtaccg acgacatgga gtaaaaatgt ccagaccaac tatcatcatt aacgacctgg
    12481 atgccgaacg catcgatatt ctgctggagc aacccgccta tgctggtttg ccaatcgccg
    12541 acgcgttaaa cgcagagttg gatcgcgccc aaatgtgttc gccagaagag atgccacacg
    12601 acgtggtgac aatgaacagc cgggttaaat tccgcaatct tagcgatggc gaagtgcgtg
    12661 tgcgcacgct ggtgtatccg gcaaaaatga ccgacagcaa tactcagctt tccgttatgg
    12721 ctccggtagg tgccgcactg ctggggctgc gcgttggcga ttccattcac tgggaacttc
    12781 cgggcggcgt tgcaacccac cttgaagtgc tggaactcga ataccagcca gaagctgctg
    12841 gcgactacct gctttaatcc catctgcaca agcgataact cgctttgccc agaggatgca
    12901 ctcgcatcct ctttccgctc aaatatcaac cagatttatc attttgctct taagcgcagc
    12961 attctaatct gaactggcta ttcatctgcg gaacctcata tgagcgaagc tctcggtatc
    13021 actgcatttt atatattcat agcagcaatt attgttgctg cagtgctata ccttgaacaa
    13081 cgctggtagc gcctggtttt tatttatttt ccagctcaat agcaaattca gatcaaatca
    13141 gcctcgcaaa ttatagattc gtaaccaata atgtgctgtt atgtatacat gcaaaaacaa
    13201 ggagagaatg cttatgtaca aaacaatcat catgccagtg gatgttttcg aaatggaatt
    13261 gagcgacaaa gcgattcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct
    13321 acttcacgta ctaccggggt cagccagcct gagcctgcac cgttttgccg ctgatgtacg
    13381 tcgttttgaa gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca
    13441 cttcaccatc gatccttccc gcattaagca acatgtccgt tttggtagtg tgcgggatga
    13501 agttaacgag ttagcgaaag aacttgatgc agatgtagta gtgattggtt cgcgtaaccc
    13561 atcaatttca actcatctgt tgggttctaa cgcctcgagc gtaatccgcc acgccaattt
    13621 gccggtgctg gttgtgcgtt aattgccaga ataagtctcc cgccctgccc ggagccatcc
    13681 ctgcagggct ttttaatgca ataaaaaagc cgccaggttt gaccccggcg gctaagcaat
    13741 tacaggtgaa tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac
    13801 tcagagaggc tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg
    13861 tacagtcacc cgctgcgaac acgcctttga cgttggtttc gcatttcgca tcaatgataa
    13921 tctcgcccat gcgattacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac
    13981 caatctgcac gaaaataccg gccagttcga tgttgtgaat atcgccgctg acacgatctc
    14041 gatattccag acctacgact ttgctaccgt cgcctttcac ttccgtggtt tgcgcattca
    14101 gaataatgtc gacgtttttc aggctgcgca gtttgtcctg cagaacctgg tcggctttca
    14161 tttctggcgc aaattccagc agcgttacgt gctcaacgat acccgccagg tcaattgccg
    14221 cttccacgcc ggagttaccg ccgccgataa ccgctacgcg tttaccttta aacagcgggc
    14281 cgtcgcagtg cgggcagtag gtcacgcctt tggtgcgata ctgatcttcg cccggaacgt
    14341 tcatgttgcg ccattttgca ccggtcgcca cgataatgct gcgtgctttc agtaccgcgc
    14401 cagaagctgt ttcaatctga tgcaggccac cttcaaccgc tgccgggatc agtttgctgg
    14461 cgctctggct gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agcgctcctg
    14521 ccagtttctg gccttcggtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga
    14581 ggatctgacc accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt
    14641 aaattgctgc cgctgcaccc gccgggccgg aaccgacgat taatacgtca taagcatcac
    14701 gcttgttcag ctcttctgcc gcacgttttt ccgcaccagt atcaattttg gcaacgattt
    14761 cagtcaacgt catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc
    14821 ccatcacgtt gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct
    14881 tgatgcgcgg gttcagtacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttgt
    14941 ggcaagagag cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct
    15001 ccagcagaga ctgcgcttct ttcgacggat gaccaccggt ccacagcaac gccagtacca
    15061 gcgaggtgaa ctcgtggccc agcggggagc ctgcaaaacg tggcccctgg ttagaacctg
    15121 ggttggtgat caggaaagac ggcttacgca ccggcaagct gttatcttct ttaaaggtga
    15181 ctttgtctga cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc
    15241 tgtcatccag cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga
    15301 gttgagtttt catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc
    15361 aagcggcctc tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg
    15421 tgccgcagca cccgaagaat taggtgaatt tccgaagtat ttcgcgccgg agttagtagc
    15481 gcgaaagaca aaggaaattt agattttacc aaccaggtcc agagacggag ccagagttgc
    15541 ttcaccttct ttccatttag ccgggcaaac ttcacctggg tgagaagcta cgtactgtgc
    15601 tgctttgatt ttacgcagca ggtcagacgc gtcacggcca atgccttcag cggtaacttc
    15661 gattgcctgg atgataccct gcgggtcaac aacgaaggtc gcacggtcag ccagaccttc
    15721 atcttcacgc atgttgtcga agttacgggt cagggcgcca gtcgggtcgc cgatcatcgc
    15781 atatttgatt ttagcgatgg tttcagagct gctgtgccat gctttgtggg tgaagtgagt
    15841 atcggtagat actgcgtata cgtctacgcc cagtttctgc aattcttcgt aatggtcagc
    15901 aacgtcaccc agttcggtcg ggcatacgaa agtaaagtca gccgggtaga agaagaagac
    15961 gctccagcgg ccttcggtat ctttttcggt gatttcgatg aattcgccgt ttttgaatgc
    16021 ctggttttta aaaggtttaa ttttggtgtt aatcaaggac atctatactt cctccgtgtt
    16081 ttcgatgaga tgtaaggtaa ccaatttttg ccgattcggc taatgcgttt ccattatcaa
    16141 atcgataagt tttaccttac aaccttcgta agacaacttt gtgaactttg caccgccagc
    16201 ggcgataaaa agtaaaatgg ccccctttcc aggtgaccgt gtaatctgaa ctaccctttg
    16261 gtatcttcag cgcctgcctg caggctgagg tgttgccctt acaaatgcaa caacgacatg
    16321 gattacaaca ccctcataaa caaagggcaa tcacctgatc taagctctta cctatgacag
    16381 tgataggtta tgccttttac tcgacttttg cactgactga aaaggacaaa ttaatgttaa
    16441 aaaagatact tttactggct ctgcttcctg caatcgcctt cgcagaggaa cttcctgccc
    16501 cagtaaaagc gattgaaaaa cagggtatta caatcatcaa aacgttcgat gcccctggag
    16561 gaatgaaagg ttatctcggt aagtatcagg atatgggcgt caccatctac ctgactccag
    16621 atggtaagca cgctatctct ggttacatgt acaacgaaaa aggtgaaaac ctgagtaaca
    16681 cgcttatcga aaaagaaatt tacgcacccg ccggacgtga aatgtggcaa cggatggaac
    16741 aatcccactg gctcctcgac ggtaaaaaag atgcgccggt cattgtctac gtcttcgccg
    16801 atccgttctg cccatattgt aaacaattct ggcagcaggc tcgtccgtgg gtagattctg
    16861 gcaaagtgca attaagaaca ttgttggttg gggttatcaa gccagaaagc ccggcgacag
    16921 cagcggcaat tcttgcctcc aaagatcccg caaaaacctg gcaacaatat gaagcctctg
    16981 gtggcaagct taagctaagc gtgcctgcaa acgtaagcac agagcaaatg aaagtgttaa
    17041 gcgacaacga gaaactcatg gacgatctgg gggcaaatgt cacaccagct atctattaca
    17101 tgagtaagga aaataccctg caacaagccg tcggattacc cgatcagaaa atgcttaata
//
LOCUS       DAZKJR010000112        16850 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKJR010000112 DAZKJR010000000
VERSION     DAZKJR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.113"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHOWS_003720"
     CDS             complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHOWS_003720"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0049234.1"
                     /translation="MLEDLKRQVLEANLALPKHNLITLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHOWS_003721"
     CDS             complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHOWS_003721"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0049235.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGIKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHOWS_003722"
     CDS             complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHOWS_003722"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFQ0049236.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIANKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4471..5349
                     /gene="araC"
                     /locus_tag="ACHOWS_003723"
     CDS             4471..5349
                     /gene="araC"
                     /locus_tag="ACHOWS_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFQ0049237.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAAKLS"
     gene            5435..6199
                     /gene="yabI"
                     /locus_tag="ACHOWS_003724"
     CDS             5435..6199
                     /gene="yabI"
                     /locus_tag="ACHOWS_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFQ0049238.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLAPLISAVGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHOWS_003725"
     CDS             complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHOWS_003725"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFQ0049239.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERSEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASAILGITG"
     gene            complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHOWS_003726"
     CDS             complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHOWS_003726"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFQ0049240.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHOWS_003727"
     CDS             complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHOWS_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFQ0049241.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9595..9694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHOWS_003728"
     CDS             complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHOWS_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFQ0049242.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHOWS_003729"
     CDS             11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHOWS_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFQ0049243.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHOWS_003730"
     CDS             complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHOWS_003730"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFQ0049244.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHOWS_003731"
     CDS             complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHOWS_003731"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFQ0049245.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHOWS_003732"
     CDS             complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHOWS_003732"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFQ0049246.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHOWS_003733"
     CDS             complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHOWS_003733"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFQ0049247.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHOWS_003734"
     CDS             complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHOWS_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFQ0049248.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRLVSGIQN"
BASE COUNT         3715 a         4586 c         4587 g         3962 t
ORIGIN      
        1 ttggctgtgg ttttatacag tcattactgc ccgtaatatg ccttcgcacc atgcttacgc
       61 agatagtgtt tatccagcag cgtttgctgc atatccggta actgcggcgc taactgacgg
      121 cagaatatcc ccatataagc gacctcttcc agcacgatgg cgttatgcac cgcatcttcg
      181 gcatttttgc cccatgcaaa tgggccgtga gaatggacca ggacgccggg catttgtgct
      241 gcgtcgattc cctgtttttc gaaggtttct acgatgacgt taccggtttc ccactcatat
      301 tcaccgttga tttctgcgtc ggtcattttg cgggtgcagg gaatggtgcc gtagaaatag
      361 tcggcgtggg tggtaccggt tgccggaatt gactggcccg cctgcgccca gatggtggcg
      421 tggcgcgagt gtgtgtgcac aatgccgcca atggacggga atgcctgata gagcagccgg
      481 tgagttggcg tgtcggagga gggctttttc gttccttcaa ccacttcacc ggtttcgatg
      541 ctgaccacga ccatatcgtc agcggtcatg acgttgtaat cgacgccgga aggtttgatc
      601 accaagacgc cgcgctcgcg atcaacggcg ctgacattgc cccatgtgag cgtgatcagg
      661 ttatgttttg gcagcgccaa attggcttct aatacctggc gtttgagatc ttctaacatg
      721 ttgactcctt cgtgccggat gcgctttgct tatccggcct acaaaatcgc agcgtgtagg
      781 cctgataaga cgcgtcagcg tcgcatcagg cggtgaatgc cggatgcgct ttgcttatcc
      841 ggcctacaaa atcacagcgt gtaggcctga taagacgcgt cagcgtcgca tcaggcgtta
      901 cacacctgat gcggctactt agcgacgaaa cccgtaatac acttcgttcc agcgcagcgc
      961 gtctttaaac gctggcaggc gggtatcgtt atcaatcacc gtgatttcaa tgtcgtgcat
     1021 ctcggcgaac tggcgcatat cgttgaggtt cagcgcatgg ctgaaaacgg tatggtgcgc
     1081 gccaccagca aggatccacg cttcggaagc agttggcaga tccggttgcg ctttccacag
     1141 cgcattcgcc accggcagtt tcggcaggga gtgcggtgtt ttcaccgtgt cgatgcagtt
     1201 aaccagcaga cggtaacgat cgccgagatc aatcaggctg gcgacaatcg ctggaccggt
     1261 ttgggtattg aagatcaggc gggcaggatc gtctttaccg ccaataccga ggtgctgaac
     1321 gtcgaggatc ggtttctctt ccacagcgat cgacggacag acttccagca tgtgggagcc
     1381 gagcaccagg tcattgcctt tctcgaagtg ataggtgtag tcctccataa aggaggtgcc
     1441 gccctgcaga ccggttgaca tcaccttcat gatgcgaagc agggcggcag ttttccagtc
     1501 gccttcgccc gcaaagccgt aaccctgctg catcagacgc tgtacggcca gaccaggaag
     1561 ctgtttcaga ccgtgcaaat cttcaaaggt ggtggtgaac gcgtggaagc caccttgttc
     1621 caggaaacgc ttaatcccca gctcaatacg cgccgcttcc agcacgttct ggcgtttttc
     1681 gccgtggatt tgtgtggcag gcgtcatggt gtagcagctt tcgtactcat cgaccagcgc
     1741 gttaacatcg ccatcgctga tggagttcac tacctgcacc agatcgccaa ccgcccaggt
     1801 attgacggag aaaccgaact tgatctgtgc ggcaacttta tcaccatcgg tgaccgccac
     1861 ttcacgcatg ttatcgccaa aacggcagac tttcagatga cgggtatcct gtttagagac
     1921 cgcctgacgc atccaggagc cgatacgctc atgggcttgt ttatcctgcc agtgaccggt
     1981 aacgacggca tgttgctgac gcatacgcgc gccaatgaag ccgaactcgc gaccgccatg
     2041 tgcagtctgg ttcaggttca taaagtccat atcgatgcta tcccacggca gcgccgcgtt
     2101 aaactgggtg tggaattgca gcaacggttt gttgagcata gtcaggccgt tgatccacat
     2161 tttggccggg gagaaagtgt gcagccacac taccaggcca gcgcaacgat cgtcgtaatt
     2221 cgcgtcacgg caaatagcgg tgatttcatc cggcgtggtg cccagcggtt tcagcaccag
     2281 tttgcagggc agtttcgctt ccgtattcag cgcattaacg acgtgctcgg catgttgggt
     2341 gacctgacgc agggtttccg ggccatacag atgctggctg ccaattacaa accacacttc
     2401 ataattatca aaaatcgtca ttgtcgtgtc cttatagagt cggaacggcc tgggcagcct
     2461 gtgccggggc ggaagttgga agatagtgtt gttcggcgct catcgcccat tgctgatagc
     2521 ggcgataaag ctgttcaaag cgttgtgcct gttcgctgcg cggttgcagg gttttctcta
     2581 ccgcactggc cattttttgc tgggctgagg ggatgtctgc gtgcactttc gcggcgacgg
     2641 cagcgaaaat cgccgcaccg agcgcacagc actggtcaga ggcaacaatt tgcagcgggc
     2701 gattcagcac gtcgcagcag gcctgcataa tgacctggtt tttccgcgcg atgccgccca
     2761 gcgccatcac gttattgacg gcgatcccct gatcggtaaa gcactccatg attgcgcgtg
     2821 cgccaaaggc ggtggcagca atcaatccgc cgaacagcag cggagcgtcg gtagcgaggt
     2881 tcagatcggt aatcacccct ttcaggcgtt ggttagcgtt tggcgtgcgg cggccgttaa
     2941 accagtcgag caccaccggc aggtgatcca gagacggatt tttggcccat gcttcggtca
     3001 gcgccggaag cagttgtttc tggctggcgt tgatttgctc tttcagttcc ggatgctggg
     3061 cggcaagctg ttccagcggc cagccgagta cgcgaccgaa ccaggcgtag atatcaccaa
     3121 acgccgattg gcctgcttcc agaccgataa atccaggcac cacgctgcca tcaacctgac
     3181 cgcaaatacc tttcactgcc cgctcgccaa cgctctgttt gttggcaatc agaatgtcgc
     3241 aggtggaagt accgataact tttaccagag cgttaggctg tgcgcctgcg ccaactgcgc
     3301 ccatatggca gtcaaacgcg ccgccggaaa tcaccacgct ttcaggtagg ccgagacgct
     3361 gcgcccattc cgggcataag gtgcccaccg gaatatcggc agtccaggtg tcagtgaata
     3421 gcggggaagg caaatggcga ttgaggatcg ggtccagctc atcaaagaaa ctggctggcg
     3481 gcaggccgcc ccagctttca tgccatagcg acttatgccc ggcgctgcaa cgtccgcgac
     3541 gaatatcctg cgggcgggtg gtaccggaaa gcagagctgg cacccagtcg cacagctcaa
     3601 tccacgatgc ggcagattgc gccacggcgc tgtcctggcg agtcacatgc agtatttttg
     3661 cccagaacca ttcgctggaa taaataccgc caatatagcg ggagtagtca acgttgcccg
     3721 gcgtgtgaca taagcgggtt atctcttccg cttcttcaac cgcggtgtgg tctttccaca
     3781 atacgaacat cgcgttcggg ttttcggcaa actccgggcg cagcgccagg acattaccgt
     3841 ccgcgtcaat cggtgcgggc gtcgagccgg ttgtgtcaac gccaatcccg accacagctg
     3901 cgcgctgctc ggcgctaagc tctgcaagca cggttttcag cgccgcttcc attgactcaa
     3961 tgtagtcacg cggatgatga cggaactggt tatttggggc atcacaaaat tgccctttct
     4021 gccaacgggg ataccactct acgctggtgg cgatctcttc accggtggcg cagtccaccg
     4081 ccaaagctcg cacagaatca ctgccaaaat cgaggccaat tgcaatcgcc atcgtttcac
     4141 tccatccaaa aaaacgggta tggagaaaca gtagagagtt gcgaaaaaaa gcgtcaggta
     4201 ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa
     4261 taatcaatgt ggacttttct gccgtgatta tagacacttt tgttacgcgt ttttgtcatg
     4321 gctttggtcc cgctttgtta cagaatgctt ttaataagcg gggttaccgg ttggattagc
     4381 gagaagagcc agtaaaagac gcagtgacgg caatgtctga tgcaatatgg acaattggtt
     4441 tcttctctga atggcgggag tatgaaaagt atggctgaag cgcaaaatga tcccctgctg
     4501 ccgggatact cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaacggt
     4561 tatctcgatt tttttatcga ccgaccactg ggaatgaagg gttatattct caatctcacc
     4621 atacgcggcc agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat
     4681 attttgctgt tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa
     4741 tggtatcacc agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg
     4801 ccgtcaatat ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc
     4861 agcgacctgt ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg
     4921 ctggcgataa atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg
     4981 ctccatccac cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg
     5041 gcagacagca attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt
     5101 ctgtcgcatc tgttccgcca gcagttagga attagcgtct taagctggcg cgaggaccaa
     5161 cgcattagtc aggcgaagct gcttttgagc accacccgga tgcctatcgc caccgtcggt
     5221 cgcaatgttg gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg
     5281 gccagcccga gtgaattccg tgccggttgt gaagaaaaag tgaatgatgt agccgccaag
     5341 ttgtcataat tggtaacgaa tcagataatt gacggcttga cggagtagca tagggtttgc
     5401 agaatccctg cttcgtccat ttgacaggca cattatgcaa gcattgctgg aacactttat
     5461 tacccaatcc accgtgtatt cattgatggc ggtggtgttg gtagcctttc tggagtcgct
     5521 ggcgctggtc ggtttgattc tacccggtac ggtgctaatg gcgggactgg gggcgctgat
     5581 tggcagtggc gagttaagtt tctggcacgc ctggctggca gggatcgtcg gttgtctgct
     5641 gggcgactgg atctccttct ggctgggctg gcgttttaaa aagccgttgc atcgctggtc
     5701 atttctgaag aagaacaaag cattactcga taaaaccgaa catgcgctgc atcagcacag
     5761 catgttcacc attctggtcg gtcgctttgt tggcccgacg cgtccgctgg tgccaatggt
     5821 ggcgggaatg ctggatctgc cggtggcaaa attcattacg ccgaatatta tcggctgcct
     5881 gctgtggcca ccgttttact tcctgccagg gattctggcg ggtgcggcga tcgatattcc
     5941 agccggaatg cagagcggcg agtttaaatg gttgctgctg gcgacggcgg tatttttgtg
     6001 ggttggtggc tggctgtgct ggcggttatg gcgcagcggt aaagcgactg accgtttgag
     6061 tcattatttg tcgcgcggtc gtttgttgtg gctggcaccg ttaatttctg ccgtcggtgt
     6121 agtggcgctg gtggtgttaa ttcgccaccc gttgatgccg gtgtatatcg atattttgcg
     6181 taaagtggtt ggggtttagg ggatagcctt gtgcgggttg cctgatgcga cgcttgccgc
     6241 gtcttatcag gcctacaaaa cgcactgtcc gtaggtcgga taaggcgttc acgccgcatc
     6301 cgacagtgca tactaacccg taatccccaa tattgccgaa gcactcgcct taccgctcaa
     6361 caactcatcg gtcataccct gccaggcgat gcgcccgtcg gcgaccacca ccgagcgcgt
     6421 ggcgatccgc gccgcatctt ccacactgtg cgacaccatc aatagcgtca ttttttgctg
     6481 ttggcagctc gtgctcacca gcgtcaacat ctcctgacgt aacgccggat cgagcgcaga
     6541 gaacggttca tcgagcaata aaatcggctg ttcgcgcacc agacaacgcg ctaacgccac
     6601 gcgctgtcgc tgaccgccag aaagctcgcc cggtaagcgc gccattaaat tatcaatccc
     6661 catctgacgg gcaatcgcgt gcattttctc ctgttgtgcc gcgttcagct tcaatcctgg
     6721 attaagcccc agcccgatgt tctgcgcgac cgtcaggtga ctgaacaggt tgttttcctg
     6781 aaacagcatc gacaccggac ggcgtgacgg cggcgtcgtg gtgtgatcaa cgccgtcgat
     6841 agtcagcgaa ccgctggctg gcgtcagaaa accggcgatc aaattcagca gggtactttt
     6901 acccgcgccg cttggcccga ggatcgccac ctgctcgctg cgttccaccg ttaagctaaa
     6961 acgcatcggc aaatggtggt aaagccaggt gatatcagtc agttttaaca tttcgccccg
     7021 gtagtttttc aatcacggta aacagcagaa aacagagtag cagcagaatt aacgcggtga
     7081 ccgcgccgtc ctggctgcga taggagccaa tttgctggta gagataaaac ggcagggtgc
     7141 ggaaatcatc gttaccaaac aacgccacca cgccaaaatc accaatcgat agcacacaag
     7201 caaaggccag tgcctgcgcc agtgggcgtt taagggcgcg cagctccacc acttttaagc
     7261 gtgaccagcc ttcaatgccc agcgactgac acaacatact gtaacgcgcg gtgatatcgc
     7321 gcatcgggtt ttccagcact ttcagcgcat aagggatcgc cattaacgca ttggtgaaaa
     7381 tcacaatgcc gtcagcagat tgtggcaggc cgatagtgtt gttgagcagt aaaaagaagc
     7441 cggtagccag cacaatcccc ggcatcgcga ggatcaacat gccgctcatc tccagcgcct
     7501 gacccgccag cattttctgc cgcgcccgca gttcgcgact gctccatagc agcatcatgg
     7561 tcagcactac gcataatacg cctgccgcca gcgcaatacg caacgaggtc cacagcgcct
     7621 gccacagcac cggctgcgcc agcacttccg gcaactggcg atttatccca tcgacgagca
     7681 ccgccagtaa cggtggcagc aacagcaaca gcgccagcac aattaacacc gtgtcgcaaa
     7741 tgcggctatg cagacgatcg tccgggtcgc gccagccttg cagcagcgta gtgccgggcg
     7801 caatggcctt actcaatcgc tgactcaaca gcaccagccc aaggcaacac accatctgga
     7861 tcagcgccag catcgccgcg cgggcaggat cgtagtcgta actcagcgcc tgataaatag
     7921 ccagctcgat agtcgtcgct tgcggaccgc cgcccagcga caacacggtg gcgaagctgg
     7981 cgaaacagag cataaagata agcgcggcaa ccggcgggat ttgtcgccgt aaccacggcc
     8041 attcgacgaa gcggaaaaaa tgccagccgc gcatcccaag ctgggcggca agctgacgtt
     8101 gttccccggg aatgttttcc agtgcctgga gtaataagcg gctcgccatc ggcagattaa
     8161 aaaatacatg cgccagcaaa atgccttgca ggccgtaggg cgaaaacgtc cactccagac
     8221 caagcgattg ccagagcgat gccagccagc cctggcgacc atagacgcta agaatgccga
     8281 aaacagcaac cagcaccggg aggatcaagg tcatggcaca cagacgcaac agcgccagcc
     8341 gtcctggaaa gcgcctgcgg tagagggcgc gggcaaggaa tatcgcgggc acgacagaga
     8401 gcagcgccga gagaaacgcc tgccagaagg agaagcgcac cacatgccac aggtagctgt
     8461 cctgccagat tgccacccag tcaccctgcg gcgcgttcca ccacagggcg agaaacgcag
     8521 ccagtgcaac cgccaccacc agcgtggcgg cgcttattcc tggaattaac cagccgggaa
     8581 ttaacggctg acggcgcgtt gccattcgct aatccatgcc tgacgttgtg ccgccacttc
     8641 ggctggcgtg aactccagcg tggttgccgg tttggtcaat tgttcaaaac cggcaggcag
     8701 cgtgacgttt gccaccggat acatccagtt gccggttggg atcgcattct gaaaagccgg
     8761 agaaaccata aactggagaa atttttgcgc cagctccggc tgcttgctgg cagcggtgcg
     8821 ggcggcgact tccacctgca gatagtgacc ttcgctgaag tttgcggcgg cgtagttatc
     8881 tttcttctct tcgagaatgt gataagccgg agaggtggtg taactcagta ccagatcgct
     8941 ttcacctttt aaaaacaggc cgtaggcttc gctccagcct ttggtgaccg tgaccgtttt
     9001 cttcgccagt ttctgccagg cttgtggggc gtcatcgcca tagacttttt gcatccacaa
     9061 cagcaggccc agccccggcg tactggtgcg cggatcctga taaatcaccc gccagttttg
     9121 atcgctctca acaagctctt tcaggctttg cggcggattt ttcagtttgt tcttgtcata
     9181 gacgaaggcg aaatagccgt aatcaaacgg tacgaaagtg tcatttttcc agccgccggg
     9241 aacgttaacg gcatccgctg ccacgccgct tttggcaaat agtccggttt tactggcggc
     9301 gtctaacagg ttgttatcca gccccagcac cacatcggct ttactgtttt tgccttccat
     9361 ccgcagacgg ttgagcagcg aaacgccatc ttccagcgcc accagtttca gttcgcagtt
     9421 acagtcggct tcaaaagctt ttttgatctt tggaccaggc ccccagtcgg cggcgaagga
     9481 atcgtaggta taaacagtca gaacgggttt agcgaaaacg ggcgctgtgc acagcaacag
     9541 caaggggaga cattttttta acactttgca cctcaaaaaa gagtggcaaa ggacttgaga
     9601 aggagcctca aatcccttcg ccggcgttat ccggatcagg ttcgacgggt attttctcag
     9661 cgcacgcgtc tgcgtggcac cccgttgaga acggcgttag tgtagtgatt ttgttatcaa
     9721 ccagcaatca tggatccggc ggcgcaaacc acgctgattt aaaatcgaac cagccgaggg
     9781 tattcatacg caggccgcgc atactgcgtt gcccctgaat gatcagccag tggtggataa
     9841 gcggcaccat tgctttgctg gcgaccagtt gctggcacca gttcgccagg tttatttcgc
     9901 cattgcgcca gcgggcggcg tcggcttgcc agtcgatcgg aatacagtgt tgtagcagcg
     9961 gcacctcgca caggtgcgcg aatagcgaaa aatccagcgg cagcgtaaag ttggcgctgt
    10021 taagccagat atcgctctcg atctctcctt catgccactg atcgtagctg atctctttga
    10081 tttccagcgt gacctggtga cttgccagaa tctgctgcat gatcccggca atcaccctat
    10141 gctcactgtg atcctgataa aaggtcaggg tgaggctttc cagaccagcc ggtttttcgc
    10201 tctttatggt gcgggtatgg tgccaacggg gaagcagccc ataggccgga aaccacaact
    10261 gctggtactg ttcctcggcg aaataaacca gattggtcgg cgaaagcaca tagcttatcc
    10321 agtctctgac ttgctgattc gccccgcgat gggtacggct gtcgaacagt aaatagtaac
    10381 aaccttcttc aaggcggctt tcaatctctt tttcctcgtc ttgcggtcct ttcagcatca
    10441 ggcctcccgc ggggtcatca gcaatttccg gcaagaccca gacgttaact tcgtcgatta
    10501 atgcccgata accaaagaag tcgtcgaatg cctgaatttt cagttgattg ctgctgttgc
    10561 ggatcaccgc ataaggaccc gttccgatgg gatggctggc aaagttgctg agggtttccc
    10621 attcgcgcgg caggatcatc gccggaactt gccccagcag taacggtaac cagcggtccg
    10681 gttgcgtgag atggatatcc agcgtccagg gcgtcggcga cacaatgtca gcaatatgcg
    10741 aatagagcgg cagcgtattg attcgtttta aagaggcgat cacgtcgtcc atttccagtt
    10801 cacgaccatg atgaaaatgg actcctggac gcaaaaagaa acgccagtga agcggtaaaa
    10861 tttgctgcca gtggtgggcg atgtccgctt ccagttcccc attttcctca tttatgcgcg
    10921 ttagcgaact gaagatttgc cgggcgatat gggtttcgga acgacgcaat gcactgccgg
    10981 gcagcagatt acgcaacgga cgatagtaga gaacgcgcag gatgtgccgc ccctggcgga
    11041 agctgcgtcc cagatgagaa accaacattt gccgcacagc cgctttgtcg ccaaccagtt
    11101 gcaccagttg atcgatacga tcctgctcca gcaggtcctc tgctcgctgt tgctgaagcg
    11161 ccagcccggt atagaggaat gtcagacgcg agcgtttgcc gcgcccgact tccgcttccc
    11221 acgtcagcca gccgcgatcc tgcatggtgt tgagcagggt gcgcatatga cgacgcgagc
    11281 agctcaataa cgctgccagt tcgttgagcg tcgtgtcctg cgatttaccc tcgcagcatt
    11341 gccacaggcg gatgaactgt tgttgcagac gagcagatgg cataaaaggg gaactcctgc
    11401 gcaaaagaca gcaattttat tttccctata ttaagtcaat aattcctaac gatgaagcaa
    11461 ggaggtgcct catgcgtcag ttttatcagc actattttac cgcgacagcg aagttgtgct
    11521 ggttgcgttg gttaagcgtc ccacaacgat taaccatgct tgaagaactg atgcaatggg
    11581 aggggagtca ttctgactac tgatttgcgg acatcatgtg tgactgagta ttggtgttaa
    11641 tcgccacgcc agcagtgatt atctgctggc ttttttcttt ttggcgattg agtgccggac
    11701 ggtgtacaaa ctaacgcata aacgccggtt gcttctcttc ataagacgca attgcgtcgt
    11761 cgtgctgcag agtcaggccg atgctgtcca gaccgttcat catgcagtgg cggcggaagg
    11821 catcgatggt aaagcgataa gttttctctc ccgctttcac ctcttgcgct tccaggtcta
    11881 catcaaaatg aatccccgga ttggcctgca ccagcgcaaa cagttcgtcc acttctgcgt
    11941 cgcttaattt caccggcagc agctggttgt taaagctgtt gccgtagaag atgtcagcaa
    12001 aactcggtgc aatcaccact ttaaaaccgt aatcggtcaa tgcccacggc gcgtgctcac
    12061 gcgaggaacc gcagccgaag ttttctcgtg ccagcaaaat ggaagcgccc tgatactgcg
    12121 ggaagttcag cacgaagtcc gggtttggct gttggccttt ttcatccaga aaacgccagt
    12181 cgttaaacag atgcgcgcca aaacccgtac gggtcacttt ctgcaaaaac tgtttcggga
    12241 tgattgcatc ggtatcgaca ttggcggcat ccagcggaac caccaggcct gtgtgtttga
    12301 taaatttctc tgccatggtg tgctccttat ttaatgttgc gaatgtcggc gaaatgtccg
    12361 gtcacagcag cagcggcagc cattgccggg ctgaccagat gggtgcgccc tccacgcccc
    12421 tggcggcctt caaagttacg gttgctggtg gaggcacaac gctcacccgg attcaggcga
    12481 tcgttgttca tcgccagaca cattgagcaa cctggcaagc gccattcaaa accggcttca
    12541 ataaagattt tgtccagacc ttccgcttcc gcctgggctt ttaccgggcc agagccggga
    12601 accaccagtg cctgcacgcc tggcgcgact ttccgtcctt tggcgatttc agctgccgcg
    12661 cgtaaatctt caatacgtga gttagtgcag gaaccgataa acactttgtc gatagccact
    12721 tctgtcagcg gaatacccgg tttcagcccc atataggcca gcgctttttc tgccgacgcg
    12781 cgttcaaccg gatcggcaaa cgaagccgga tcgggaatgt tgtcgttcac ggaaatcacc
    12841 tgacctggat tggtgcccca ggtgacctgc ggtgaaattt cttctgcctg caaagtcaca
    12901 acggtatcga aagttgcgcc ttcgtcggtt tgcagagttt tccagtaggc aacggcgtcg
    12961 tcgaaatctt tgcctttcgg cgcatgcaga cggcctttga catagttaaa ggtagtttcg
    13021 tccggtgcaa ccagaccggc tttcgcgccc atttcgattg ccatattgca cagggtcata
    13081 cgaccttcca tgcttaaatc acggattgct tcgccgcaaa actccaccac atgcccggtg
    13141 ccgcctgcgc taccagtttt accgataatc gccagcacga tatctttcgc tgtaatgccc
    13201 ggcgcggctt tgccctggac ttcaattttc atggtcttcg cacgaccctg tttcagggtt
    13261 tgcgttgcca gtacgtgttc aacttcggaa gtgccgatac caaaggctag tgcgccaaac
    13321 gcgccgtggg tggcggtatg cgagtcgccg cagacaatgg tcatccccgg caatgtgacg
    13381 ccctgttccg gccccattac gtggacgatc ccctgatacg ggtgattcag gtcatacagc
    13441 tcgacgccaa attctttgca gtttttgatc agctcctgca tctggatgcg cgccatttca
    13501 ccgcaggcat taatgtcttt ggtctgagta gagacgttgt gatccatagt ggcgaaggtt
    13561 ttgcctggct gacgtaccgg gcgaccgtgg gcgcgcagac catcgaacgc ctgcggtgag
    13621 gtcacttcat gcaccaggtg gcggtcgata tataacagcg gggtttcgtt ttcggcttcg
    13681 tacactacgt gagcgtcgaa caatttttcg tataacgttt tagccatgat tacacccctt
    13741 ctaccacata gcgggcaatg atatcgccca tttcattggt actaacggcg gcagcgccac
    13801 gggctaaatc cccggtgcga atgccttctt ctaatgcgcg gttaatggcg cgttcaatgg
    13861 cgcaagccgc atcatctgca tccaggctgt agcgcagcag cagtgccagc gacagaattt
    13921 gcgcaatcgg gttggcgatg tttttgcctg cgatatctgg tgctgagccg cctgccggtt
    13981 catacaaacc aaaaccttgc tcgttcaggc tggcggaagg caacatcccc atcgagccag
    14041 tgatcattgc gcactcgtca gacagaatgt cgccaaacag gttggaacac agcaggacgt
    14101 caaactgtga tggatcttta atcagctgca tggtggcgtt gtcgatgtac atatgcgcca
    14161 gttcgacatc cgggtattcc gtggcgatct cgttgacgat ttcacgccat aaaatagagg
    14221 attgcagcac gttggcttta tcgatcgacg tcactttatg gcgacgttta cgagcagatt
    14281 caaacgcgat gcgggcaata cgctcgatct caaaacggtg atacacctcg gtatcaaacg
    14341 ctttttcata ttgtccgcta ccttcgcggc cttttggctg accgaaatag atgccgccgg
    14401 tcagttcacg cacacacagg atgtcgaagc cgttagcggc aatgtcagca cgcagtggac
    14461 agaatgcttc cagcccctga tacagttttg ccggacgcag gttgctgaat aatttgaagt
    14521 gcttacgcag aggcagcagc gcgccgcgtt ctggttgctg gtctggtggt aaatgttccc
    14581 acttcggacc gcctaccgag ccaaacagca cggcatcggc ttgctcacaa ccttcaaccg
    14641 tcgcaggcgg cagtggttgc ccgtggttat caatggctgc gccgcctaca tcgtaatggc
    14701 tggtggtgat acgcatcgca aatcggttgc gcacggcatc cagcactttc agcgcctgag
    14761 tcatcacttc cggaccaata ccgtcccccg gcaatacggc aatatggtaa ttcttcgaca
    14821 tcacacggtt tccttgttgt tttcgttgtg ttgagctttg cgttgcaact ctttttcgac
    14881 ttctgcggca cgccagatat tgttcagtac gtgcaccatg gctttggcgg aggactcgac
    14941 aatatcggtg gccaggccga cgccgtggaa gcggcgaccg ttgtagttgg cgacaatatc
    15001 cacctgaccc agcgcatctt taccgtgacc tttggcggtc aggctgtatt tcaccagttc
    15061 gacgttatag tcagtgatgc ggtttatcgc ctggtagacg gcatcgaccg gaccgttacc
    15121 gttggcggct tctgctttga cttcttcgcc gcaggccagt ttgacggcgg cagtggcaat
    15181 atcgttagaa cctgactgca cgctgaagta atccagacgg aaatgctccg gctcttcttg
    15241 ctgcttaccg atgaaggcca gcgcctccag atcgtaatca aacacctgac cttttttgtc
    15301 cgccagcttc aggaaggcat cgtacaaatt gtctaaatta tattcacttt ctttataccc
    15361 catctcatcc atgcggtgtt ttactgccgc acgccccgaa cgagaggtca gattcagctg
    15421 gatttggttc aaaccaatag attctggtgt catgatttcg tagttttcgc ggtttttcag
    15481 cacgccatcc tggtggatac cggaggagtg tgcgaatgcg ccgctgccaa caatggcttt
    15541 gtttgccggg atcggcatat tacaaatctg gctaactaac tggctggtgc gccatatctc
    15601 ctggtgatta atagcggtgt ggacgttgag gatatcctta cgaactttga tcgccatgat
    15661 gacttcttcc agcgaacagt taccggcacg ctcgccgatc ccgttcattg caccttccac
    15721 ctggcgtgca ccggcatgta ccgccgccag tgagttgccg accgccaggc ccagatcgtc
    15781 gtgggtatgt acggaaataa tggctttgtc gatgttgggt acgcgttcat acagcccgct
    15841 gatgattccg gcgaactcaa atggcatggt gtagcccacg gtgtccggaa tgttgatggt
    15901 ggtggcaccg gcattaatcg ccgcttcgac cactcgcgcc agatcggcaa ttggggtgcg
    15961 tccggcatct tcgcaagaaa attcaacatc atcggtgtaa ttacgggcgc gtttcaccat
    16021 atagatagcg cgttcgatca cttcgtccag cgtgctgcgc agcttggtgg cgatgtgcat
    16081 tggcgaagtg gcaataaagg tatgaatacg gaaggcttcg gcgactttca gggattcggc
    16141 tgccacgtca atatcttttt ccacgcagcg agctaacgcg catacacggc tatttttaac
    16201 ctggcgagcg atggtctgca ctgattcaaa atcacccggc gaagagacgg ggaaaccgac
    16261 ttccatcacg tcaacaccca tacgctcaag ggccagcgca atttgcagtt tttctttcac
    16321 actcaagctt gcctgtaacg cctgttcacc gtcgcgcaat gtggtatcga aaataatgac
    16381 ttgctggctc atggtttggg tccttgtctc tttttgagcg cctcgcttcg ggcataaaaa
    16441 aacccgcgca atggcgcggg ttttttgttt gactgagtgc tgacttaatt ctgaatgccg
    16501 ctcaccagtc taccgcgcaa agaagatgcg tttagtagta gtagaccgat aaagcgaacg
    16561 atgtgagtca ttaaatcagc tccagatgaa tgcgatatgc ttttagagtt actggatacc
    16621 aaaacggatg tcaaccctga tgcaacaaaa acgtccagcc agcgtgagtt ctgcatccgt
    16681 caaattagct aattgtgctg cggtggttaa aacaagcgat attaatttct gcttaaccac
    16741 cgccgctttt catcggttga catatttcag aataaatttt tgcatctaat cgacgaggga
    16801 aagggggata aaatgcacgc gttacaaaac ctatcctgat gatttgtatt
//
LOCUS       DAZKJR010000113        16701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKJR010000113 DAZKJR010000000
VERSION     DAZKJR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.114"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..269)
                     /locus_tag="ACHOWS_003735"
     CDS             complement(<1..269)
                     /locus_tag="ACHOWS_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016808259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2895 family protein"
                     /protein_id="HFQ0049249.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYP"
     gene            complement(266..628)
                     /locus_tag="ACHOWS_003736"
     CDS             complement(266..628)
                     /locus_tag="ACHOWS_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFQ0049250.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGGIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            complement(641..955)
                     /locus_tag="ACHOWS_003737"
     CDS             complement(641..955)
                     /locus_tag="ACHOWS_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFQ0049251.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            complement(1032..1274)
                     /locus_tag="ACHOWS_003738"
     CDS             complement(1032..1274)
                     /locus_tag="ACHOWS_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049252.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            complement(1274..1615)
                     /locus_tag="ACHOWS_003739"
     CDS             complement(1274..1615)
                     /locus_tag="ACHOWS_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFQ0049253.1"
                     /translation="MFSFPASPCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            1597..1911
                     /locus_tag="ACHOWS_003740"
     CDS             1597..1911
                     /locus_tag="ACHOWS_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049254.1"
                     /translation="MPEKKTYAPPSELNSAYRMRKAEDWISDKLYLQNRKFLRENTLV
                     WMKANNPGNTGNTGNTGNTGNTGNTGNTGNNKLCSGGCQHSQFLHLAVPDSVQINLFQ
                     PE"
     gene            complement(2275..3036)
                     /locus_tag="ACHOWS_003741"
     CDS             complement(2275..3036)
                     /locus_tag="ACHOWS_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFQ0049255.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLCEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCIFY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(3029..5125)
                     /gene="traD"
                     /locus_tag="ACHOWS_003742"
     CDS             complement(3029..5125)
                     /gene="traD"
                     /locus_tag="ACHOWS_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFQ0049256.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(5122..5910)
                     /locus_tag="ACHOWS_003743"
     CDS             complement(5122..5910)
                     /locus_tag="ACHOWS_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049257.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEALLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            complement(5907..6389)
                     /locus_tag="ACHOWS_003744"
     CDS             complement(5907..6389)
                     /locus_tag="ACHOWS_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049258.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(6386..6979)
                     /locus_tag="ACHOWS_003745"
     CDS             complement(6386..6979)
                     /locus_tag="ACHOWS_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFQ0049259.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            complement(6976..7494)
                     /locus_tag="ACHOWS_003746"
     CDS             complement(6976..7494)
                     /locus_tag="ACHOWS_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFQ0049260.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(7491..8171)
                     /locus_tag="ACHOWS_003747"
     CDS             complement(7491..8171)
                     /locus_tag="ACHOWS_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFQ0049261.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEEQK"
     gene            complement(8150..8869)
                     /locus_tag="ACHOWS_003748"
     CDS             complement(8150..8869)
                     /locus_tag="ACHOWS_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049262.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPSILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(8873..9622)
                     /locus_tag="ACHOWS_003749"
     CDS             complement(8873..9622)
                     /locus_tag="ACHOWS_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049263.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(9619..10218)
                     /locus_tag="ACHOWS_003750"
     CDS             complement(9619..10218)
                     /locus_tag="ACHOWS_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFQ0049264.1"
                     /translation="MKKRFILSGLTVCLLMAGCQGTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(10404..11396)
                     /locus_tag="ACHOWS_003751"
     CDS             complement(10404..11396)
                     /locus_tag="ACHOWS_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFQ0049265.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEGIEKGRLEGIEK
                     GRQEGIEKGRQEEREKALQENYASARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            complement(11445..12005)
                     /locus_tag="ACHOWS_003752"
     CDS             complement(11445..12005)
                     /locus_tag="ACHOWS_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0049266.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(12021..12224)
                     /locus_tag="ACHOWS_003753"
     CDS             complement(12021..12224)
                     /locus_tag="ACHOWS_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049267.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(12315..12782)
                     /locus_tag="ACHOWS_003754"
     CDS             complement(12315..12782)
                     /locus_tag="ACHOWS_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFQ0049268.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
     gene            complement(13461..15503)
                     /locus_tag="ACHOWS_003755"
     CDS             complement(13461..15503)
                     /locus_tag="ACHOWS_003755"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0049269.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLSRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGCLITAMKNASSGVT
                     DPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
     gene            complement(15506..16210)
                     /locus_tag="ACHOWS_003756"
     CDS             complement(15506..16210)
                     /locus_tag="ACHOWS_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049270.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
BASE COUNT         3927 a         5070 c         3930 g         3774 t
ORIGIN      
        1 gggtagtcca cctctccgtt cttcggccag gcattcagtt gctgaaagat ataaaaggca
       61 aaggaataaa ccgttgacgg cgggacttcc caccagggac gggtactgcc ggaacgcaga
      121 tccggcggat tgtgaattgt cagtctgtcc ggtgccgtca tccacccgat acagaccagc
      181 atcagaattc cggccagcac accgctgaaa atacgcagtg tcaggatatg ctggtcgcga
      241 tcccgcagag catgacgaaa acgactcatt ttttccccct gctacgggta cggcgcaggg
      301 accagccatg cgcctcccgg ataaatgttt tccggccacc accccatgca ctgcttttct
      361 tcgccagatg ctgccagagc cagttgtcag gttttccccg cttcagccgg ctgatccaga
      421 cgccaccgaa ccagaccagc agcaggggag tcagcaacat ccctgtcggg ataatcaccc
      481 agccaagtag aggaatcagc ggtaacggcc agatcccccc tgccacagcg cctgtcaggg
      541 ccgccagccc cagttccggt gtggtaaaac cacggaaaac cgcaggctcc acgttcagac
      601 gatcgggcag aaaacgaatg gtctgcatgg ggatgtctcc ttacagaatg atggaggccg
      661 acttgcccag cagccagata atggcgacca gcagaacaac accaaccacc acgatggcac
      721 caaactgccc ccatccggcc ttcccgtcac gaacctctga gaaggtggaa agcgccgctt
      781 ccgccacctt cacaaaagca actgcactga gcacaagacc aaacagcaca acaccatcct
      841 ggagatagcc tttgatctgt ccgaaaagtc caccacctcc gccggatgtt ggcggctcta
      901 tctgaggcaa atcagccaga gcaggtgtga tcccggtaca cagaaacacc gccatggcat
      961 accaggcctg gcgacagatg cgcatttttt tatgcattat ttcctcctga aacttaaaca
     1021 agacactgaa atcaacccgc aaacatccag atactgcaga tcagcagcag gacgagtcgg
     1081 aacataaacc agccgaacac accatcccgg acatcaccgc tacgccagcc tgcccaggca
     1141 tccgtcagac tccacgctgc ccagaggaac acaactgcca gcagaaatcc cagcgtgatg
     1201 agatgcagca cactgagatc cacttcacct gaagcagccc ggaaggccgt attctgcagg
     1261 gccgtcatcg ccatcaggga cgctcccgcc ggtagtcacc ggtcacggct gcggaagacc
     1321 ggggttgcgc ccgggaaggg gccagataat ggtcaatacc gtcacgaatg gtctgcagat
     1381 ctgcacgggc acgcagataa tcaaaaaagt agcgttcccc ctgtgaagaa gcctgcagag
     1441 aggccgttct ggcccgctcc agcgctgcct gcacgtggtc aagctggcgc attacagtgg
     1501 ccagttcatc cttttcggca gcctgcgcag gtaatgcaaa ggacaaaaga aacagcatcc
     1561 ccgcggcacc ggccagacgg ccggcacggc agggtgatgc cggaaaagaa aacatacgca
     1621 cctccgtcag agttaaacag tgcgtacagg atgcgtaagg cagaggactg gatcagcgat
     1681 aaactctatt tgcaaaacag aaaattttta cgggagaata cactggtgtg gatgaaggca
     1741 aacaacccgg gcaatacggg caatacgggc aatacgggca atacgggcaa tacgggcaat
     1801 acgggcaata cgggcaacaa taaactatgt tctgggggct gtcagcatag ccagtttctg
     1861 catctcgctg ttccagattc tgttcaaata aatttattcc agcctgaatg atttattcct
     1921 gtttattaaa acacggcatc actactgttt cccgttaatg acacctcatt aacaatttaa
     1981 agaggaaaca catcagcatg gaatttctcc gtactgccct tccgttctgg gccctgttcc
     2041 ttctgacctg ggtttgtgtt gtgtattttt tcatttatga agaacctcgt tgccccccgt
     2101 ggatctatga cgtcatcagg gccatcactc tgttcattat tttcgtcaac attgttgtca
     2161 ttccggcgct gtcgttactc tgacactgac gcgcactgac cgtaagggcg tggcttgtct
     2221 gccgacaccc tgccacaaaa caagttttgc atcagggtgt ccggcctctg tggattataa
     2281 atatttcttg aaggatgcca tggtcacggt gatggccacc cccagcagca tggccgccgg
     2341 ccatatcagc aggttcggat acaccgacgt cggccaggac aggtaaaaaa tgcagggaat
     2401 atatatcgcc ggtttaatag acttttttgc atggtgataa acaaagctgg actcatacgc
     2461 agcaccatac cggcgcagat cacgacgccc cagtccctcc acaaccgcga tcagtatcac
     2521 catcacaaac agcggaacag acaacaccag gatcgtcacc cgtatcagtg tgaccaccgt
     2581 gacatatact gtggccatca ggtactcaca cagataatgg ctcagccagc gccccacatc
     2641 ctcactgtac tgtgacacac tgttacccac cacggcaccg gcattttgct gcttcagcca
     2701 cgacagaaaa cctgtttcca caaacagcca ctgatacgcc gttgctatcc attctgtcac
     2761 cgttaccgat ggtgtcgaca gcagcagact gcgggtgaat tccgaagaca gccaccccag
     2821 ttctgtcttc atcactgcct cactgtgtgc tgccccctgc tccggccaga aaaaagcaat
     2881 cccgatatac tcaaccagca ggctcagcag cagggaggaa aacaggatcc cgaccagcat
     2941 ccacggccag ccccacaaca gattccagag cagtccgtgc tgacgtggcg gagagggttg
     3001 tgctgctctg ttcacctcac gacggggttc agccattctg ctctccttcc ggcatgttca
     3061 cggagggagt ctcattccac catccctctc cggtgtggta attacgccgc atggcctctg
     3121 caatttttgc aatactctcc ggcatcagcg cgtcatctgc atcagttgca ggcaggggca
     3181 tacggatttt ccacaactgt cccccttcca gcagagcaaa ggcctgtcct ttcggcaggg
     3241 tcacgatatc tgccggctcc agcaaaggca ccttcacagt accaacacga tcctgggtac
     3301 tggatgtgaa gtcctggccg gtcgtgacat ctgcggtatc ctgatgaccg gaaaccagcg
     3361 ttttggtata aatttccacc tgggacaact gggtcgtcag taactcggcc gtccggttct
     3421 ccctgacccg cagcataatc atgttattaa agttaccctg cacctgggcc gttttggcag
     3481 cactaccggt acgggcctca atatcagaag aggtctgcgt gtaagccgtc acctgcatac
     3541 cggcaccgcc tcccttgttg attaacggaa tgaactcatc gcccatcagt tcattaaact
     3601 catcacaatg caggttaacc ggatttttgg taccattccc tcccggaaga ccggcattca
     3661 ttccgtgttt gtagatatgc ccggcgacac tcaccagatc cgcaaacatg gagttaccca
     3721 cggcactggc cacctcactg tcactcagcg catcaagacc aatgtacacc accgcctttt
     3781 tacgtatcac ctgctcccag tcaaacacgg gacgggtatc gctcatatcc aggtagtcag
     3841 gtgccagcag ttctgccgtc tttcctgtgg tcagtttttc cagcaacggc aacagggatg
     3901 ccacaatttt gtcaaaatag gtacgatcat aacgtaccgc gcttcgcaga ccatccagta
     3961 tcggatcata gagtttcttt ccggcttcac tgctgagagc cacctcaatg gcccagatac
     4021 gcacagcatc cggctggttc tgcatggtcc ggggaacatc cgcttccccc agtacattca
     4081 tgttattcag aatggcgttt tccagttccg gcatctgttc gctgatcacc ttaccagcat
     4141 aacggatata aaggtcggca atattattca cataacgcat aatcagcgtg taatctgggc
     4201 gctcccccag tgccaccagt gcacgggcaa caatgttgac aaaccgccat gcaaactcac
     4261 ggaaggccgc actgttacct tcaccggata actgaccggc cacacgggaa gccacctccg
     4321 acacacgacc aaaacgcccc acggcgttat aacgcgcaga gatttccggc cagcccagat
     4381 gaaagatata aagctcatcg cccctgccgg cacggtgtgc ttcagcccag acacgcagca
     4441 acagatccgc atcccctttg ggatcaaaga caatggtgac atctccccga cgaatatcct
     4501 gtgtgaccag caactccgcc agccgggttt tcccgacacg ggtggtgcca tagacaatgg
     4561 tgtgccccac acgttcaccc agtgccagcg tcacatcctg ctcatccggc tcaataccat
     4621 gcagggcagg atttcctccc accggcggca ggggacggac gggattcagc ggcgagtcac
     4681 gacgaagaag ctgcccaatc cagggaagac tgtgctccgt tttcatttca agccagcgcg
     4741 ctgtccggta cagactgccc ggctgtagat agtgtgcaac ctccggtctc agcgtgtcct
     4801 gaaggcgctg ggtgtgcttc tgtgtccagc ggaagccgcg tcccagaaac agccgctgac
     4861 ggctgaccgg aatttgccgg gaactcatca cataacgggg cagccggcgc agattgcgac
     4921 ggtaacggat gatcttcatt ccctgccgga aacggaccac tgccagaacg ccaaagccgg
     4981 cggctgtgac atagctgacc gaaggcgcga gcgccaccgc ccagggcgct gtcacacaga
     5041 caccggccgc cgttgctgcc accacggcgg tattcagttc cacagccgga cgcagcagag
     5101 cctcaatcac ataacggtta ctcatgaccg ggtacctctc tgtaaaagac gccgccacca
     5161 tgagcgcggc aacgctttct ccggtgatgc tgacagtgcc tccagtctgc gctgtaacac
     5221 catcacctcc cgccatgcgt ctgccgcatt cagcagtaat gccttataca ccagtggccc
     5281 gtcatgtccg ccggcatgca catcatccac tgtcaacagc gcctctgttc tgaaacggtc
     5341 cactttccag caggccaggt cttcgcggga aacacactgc cgccggatct gcagacgaag
     5401 atacacatca tgccgggaca acggttctgc caggttgtga atttcccgac aggcagaccg
     5461 gaacgcctgg tctgtgtgat aaatactctg ggccacattg tgaagctgtg tgcgcccctc
     5521 cccctgcagg tggaacagaa agcgggccag acagctgcgg tgattaccgt cagcactgac
     5581 ataccagtga atgccatccg tgctgacaaa acccatcccg gcatgctgtt gctctgcctg
     5641 cgtgtaatat cccggattct tttcaagtaa cgggatattg atgtccatgc gtttaccacg
     5701 ctccagcagc tccagccagc tcattccggc atactgtgga tggtcagtcc ccacaatgcg
     5761 gtacacattg atactgccct gtcccgtcca gtagtgttcc gtaataaaat ccgacattgc
     5821 cgcaaactct gcggtatccc atgcaaccag ttgttcatgt gcccaggccg gagcattcgc
     5881 ctgtatccgc tctgtaaatg cccccttcat tcagctcttc tctcctgcac ttttgagata
     5941 ccgataccgt caggccacgg cataacagcc gggccccccc tgccttcccc ggatacggct
     6001 gcagaaccac aaccacgaaa cagaatgact gacgagacgc cttccataaa caaagcgccc
     6061 agcaacagaa ccactgcctg cattttcagt tcacgaatat cctcagcaac agcctccccg
     6121 aaagaaatcg ctgttcgcgg acattcctgc tcatcgacat acatacctga gacggatatg
     6181 acggaggaac atttttgtac catcacatgc ccttcaggcg catcacgcca gcgggatgac
     6241 tccgtcgcat aacgctgtgt catgcctgcc tcaccaccac tcagattgtt ccagacatac
     6301 agcattacca gacatcctgc cagcattgac ggccagacaa tcagaagccg gagcagcagc
     6361 ataacaaacc ggtacagtgt cataatcatg atcttccttt attactcagt aaggtgccat
     6421 tcagcagttg caacagacgt tgtccactga tcagctcaat atccggccac agtgtcagcc
     6481 gtgcccggct gatgttcccc gtacgccccg tatggataaa aaagcccctg catcccttcc
     6541 ggcgcagcag ttcgccaaaa gcatcaatat gtgccggacg aatggttctt ccgtaacgtt
     6601 tagcctgtat cagccagcgt tcaccgttaa tcaccacctg accatcaaga ccaccatcgc
     6661 cgctgtatga ggggttacgg ataaccggca tcccctgttg ctcaaaagcc agcagcaaca
     6721 gttcctcaaa cacatacgga ctgattttgc gcagatattt cagccgccgg gcatcaccct
     6781 gcaattcgtt cagccgcttc attacccgtg aggccgtggc acgataacgc tgatgccggc
     6841 gtgttctcac acacgctttt tttcgccggc tgcgccacag tgccggcccc aggacaacag
     6901 ccagcaggct gagaaccagg caggctgtca gtaccggaga agcagagagc gcagcagaag
     6961 tggcagacag cggcatcatg gtgcaggtac ctgtgacagg ccgttgtccg ttatcagcac
     7021 cgggtaatga cttatctgta acctgcgggc cagtacagaa ccggatgccg gaaccatcac
     7081 cacccccggt gccagctcac gcagtacgcg cagactgctc tcatccgcca cattcactac
     7141 cattcccgcc gccttccgct ccctgagtac tgctgcattc atctgcagcc atgtgcggga
     7201 atgggcatca tcgcccacaa gaaaaagtgc accgatgccc ggtaactgaa gccggcgcgc
     7261 cacaacctct ccgggtgaca tttcaggcgt actgaccggt aacattgccg cctctccctg
     7321 cggaaaagag ggtgatggca caactgaagc gggaaccggt ggctgccggt ttatcgcctc
     7381 aaaaaagggg ctggcatccc tgccgcccag gtctgcaatc actttcagtt ctgcgccagc
     7441 tgttgtacag aacagcagca gactcagtac ataaaactgt ctttctcgtt tcatttctgc
     7501 tcctcctgtg gttcagtcca gatataacgg ggggatggtg tcagcatggc ggtcgctgcc
     7561 acatgcacat cagacggatt caggaaatta tcccggtgga gccgggcaag atgccgtctg
     7621 accacggcgg tataacgtgc ggccggcgca ccacctgccg ggtggtgata acacccggcc
     7681 acctgcagcc agctgaccgg atggcgacgc cggcattcct gcagaatgac cgctgccgta
     7741 ttcagactgg tgtaaggatc aaatgccgcc catgtggacg ggaaacgatg tccattccag
     7801 cccagattga cctgtgctat cccgacatca atacgttttg ccggataatg tttcaggaac
     7861 acctgcaatg cctgccaggc ctccagacgt gtggcataac ggtacccttt tcctgccaca
     7921 tttattgtcc agggccaggg tcgggccacg tccggctgac gaaaatcacg acgccgttgt
     7981 ttcagtactc cggacatcgc ttccggagac atggacgttt ccgtcaggga aacggcatac
     8041 aaaagctctg cgggaatacc atgggcacgg gccacccgca catacccgtc cggtacgttc
     8101 tgaacggcag cacgacagac accactgcag gccaccatca gcagaaccgt cagaatgccg
     8161 ccacgcgcca tccgtcagcc ccctgctgca gtaccaccgg catttttcct tcaccaaaac
     8221 gcagccagcg tccggcatca tgattcaggg tgatatgacg gctgcgaact ttctccacag
     8281 gtatgctgtg cgcatgcgcc cactgacgca gaatctcatc ctttccctga ctgtcgacaa
     8341 ggtagatatc aacctgccgt cccgtggcca gtacggaggc aagacgggca tcacaggtgc
     8401 cacattcctt cgccttcaca aacagcgcca gccgtccccc cgtatctccg gtaagtgctc
     8461 ctgtttttcc catactgaca ggcaagatac tgggatacag gcgcttccag gccgcattca
     8521 cctcctgctg aaacgccagc tccttctctg ttcgttcata ttcagccctg acccactttt
     8581 cggcccagac acgtcgctct gccggcgttc tggcctctat accgagtacc gtcagcggat
     8641 caagtccggg agactgtatc ccccgggggc cggacatcag tgactgataa cgccggtaat
     8701 cctcctcact caccccccac tgcccggcct gctgctgaag tccggatgtg gtactctgcg
     8761 tcaccaccgt accggtcgtc cgttgcgcct ctgacgacat attcgtctgc ccggcggact
     8821 gagccagaac ggggcacagt gccagcagat acagtatttt tcgcttcatc atctactcca
     8881 ccctgagcac cgtctgacga ccattcaccc ggaacgttgc ttcaccatgg ccggcataca
     8941 caagcgtcca cccggccaca ctctctcctt cacccagcag agccacctga gagaggctgg
     9001 tatacccccg gggagccacg gctgcacggg tactgccacc gcgcagctct gtcccggtca
     9061 gcacaaaagg agcctgccga actgtatgaa ctgcgcggga tttacgggag gacgtcaccg
     9121 gagcgggctt tttctcctcc gtttttttca tccccgcttt cctgctgtcc ggctgtgacg
     9181 gtgactccgg tactttctgc attttctcca ccagaccgct gagtgtatcc agtctcaggg
     9241 aaagcttttc ccgggcagac tcaatctgct ccagcatctc cggcagattc tccagttgtt
     9301 ccttccagga tgctgtcagc acgggctgct gctccaggac tgccaggcgc ttttccatat
     9361 ccttcagttg cttctccgtc tctgtcagtt tcattctcag gggcgcgagt tctgacgcag
     9421 aaagcatttc tcttctctgg ccttccaggg tcgtgatccg gctgtcaaga ccggtcataa
     9481 gcatcagtac gccatatacc actgccccca tgaccagccc cacagtgaca gacagtatca
     9541 acacgccggg gaaacgactt tttcttcgct ccggcgacat accagacgca ccctccggta
     9601 tctgcggctg tttctcactc attttttcag aaaccctcct gttgccttca cacccggttc
     9661 acgacccaca aaaggaacgg tgaccggaac aggagccagc tggctgaccg gtaactggta
     9721 accgtcacgc agactgtggc agaccaccct ctgcacatca tccacttcca gttgccatgc
     9781 cggccccgcc attaactgca gggcagtacg cagtctcagt ggtcctggct gatactgcac
     9841 cgccggcaga ggctgacggt acagcacgcc gttggccggt ccggtggcgc agagcgtgta
     9901 accggactgt ttcagggcat aacgcatggc atcggccacc gtgggtttca gggaggaggg
     9961 aatgcgaatg tcgatcacct gagaaagtgg atcacgctgt acagcctgcg gctctgtgct
    10021 gaccagcagg taacggtcat agcgcaccac ctccggtgcc tgagaataca catccgggct
    10081 ggcaggctgt acattacggt ttactgtgac cgacggtacc ggcatggccg catcccgttg
    10141 ctgtagtttc tgagtgccct gacatcccgc catcaggagg cagactgtca gtccggacag
    10201 gataaagcgt tttttcatcg ggtatttacc tctgcagtga taaatcagag gtcaatgtgc
    10261 agaagaagac ggggctggct caatgaacaa ctcattaccc gctggcgaaa aaaaacgcct
    10321 gcagacatgc aggcgctttg ttgacaggaa ggaaagcaac ataacaaata tgtcacaatc
    10381 ctgcggttaa cataaattac cgattaccgg agagcattac gcaaatcatc atccgagaac
    10441 tgcagtattt gtttgatttt ctctgacgat atacccatct gctgcatgcg acgggccgat
    10501 gcgtaattct cctgaagagc cttttctctc tcctcctggc ggcccttctc tataccttcc
    10561 tggcggccct tctcgatacc ttccaggcgg cccttctcaa taccttcctg gcggcccttc
    10621 tcgataccag ccagttccag tcgttcagcg atagtcatta gatcatcctc atgtcttggc
    10681 agacgttctg ccatcgcccg tataaattca ggtgaagctg aatcacattc tttcaccata
    10741 tagttaaaca atgtcaccac ctgagtgtgt gtttcctgta tctgaatcaa ctgtgacaaa
    10801 aagtcaagat gaaccatcag atccactttc ttctgccgga gcttcatcat aagcatcagc
    10861 gcagccatct gcttatagcg acggataccc tcatcatcaa gaagaccgac atcaatcacc
    10921 ctgggtggcc tgccttctcc ataaagctcc cgaccaaacg ccggatcatc cagacagtca
    10981 ggccagttca tgctgtatgg ataaggactg ggtacaccat ggtaaaaaag caccggtatc
    11041 acaacgggag cacacgcatg tgctttcagg tggcgatgta tcaccgccag aacatagcgc
    11101 atcattcgca gggccatata cttatccgcc ttgctctggt gctctgtcag cgtataaatg
    11161 tatccatccc tgccatctgt ggttttcata ctccacagaa catcacaggc aaactggcga
    11221 agatccggat ccacaaacgt ggtcggctcc agttgcaggg tgttcatatc gcaccggttc
    11281 agttgttccg ggggcaacgc gcctttcagg aaatccctcg caacagaagg acattccatc
    11341 atcgctctga acgccgcatc atgtggcgtg ggggtagtgg gtgtgttttc attcacaacc
    11401 gcctccttga attaaaagtg gggcggtaag ccccgtggtt aacatcagat gacatggata
    11461 atccgtcgtt caacgccata cccacgcccc ataaagggga tatcatcgtc aaagtccatc
    11521 ggcggcattc ccccgcttcc tgaggaagcc tgaccgtttt ttcccgttga ttgttgtgca
    11581 ttttgtggtg gttgtcccca tcctccctga gcaggagcag atgaaacatt tccaccaccc
    11641 tgctgctgac gcggagaccc cagcatctgc agggtaccat tgacactcac aacaacttcc
    11701 gtggaataac gatcaacacc attctgctga tcgaaccact tccgggttct taactgacct
    11761 tcaatataca cctgtgaacc tttacgaagg tattcaccgg caatctcagc cagttttccg
    11821 taaataacaa cacggtgcca ttcagtgtgt tccttcattt caccgctctg tttatcacgc
    11881 caggtatcag tggtggccag cgtcagattt gccactgcac ctccgccgtt ggggaaataa
    11941 cggatttccg gatcctgtcc cagatgacca atcagtatga ctttattcac tccacgcgat
    12001 gccataacaa ctccttatcc tcacacacat tcacgctgag tccagatgcc gttttccaga
    12061 gcccggactg cctgctcata atttctgaaa tactcttcgg attcccggga cacaggccct
    12121 tcgtcgcact gagtaccaat ataataacca cgagcactgc tgaggatctg cagaggtaag
    12181 gtatagcccg ctttggcggc cagaattccg cttgtcggtt tcataacatg tttccttaaa
    12241 gttaaacgat aagaaaacag acctccctga tggttggtct gttttcatca gggaggttga
    12301 gttgaaaaat acgatcagaa agaattttct gcgtattccc gcggggtatt gccattctgt
    12361 ggcggtatgg attccgattt ttcagtctta taaaccatgt cctggcctat tttgatccac
    12421 tccaccttga tcaggcgggc tttcagactg acgcgttgct caccggcatg ttcaccccgg
    12481 ttaagggtaa aaatgtcagt agaggggttg ctcatggtaa aaccaatcag caccttgcga
    12541 tcttcatcca ccgctttctg gcagcgggca atcagggaag ttgcctcttt accggcaacc
    12601 gtcacatcaa aacgcacata ggacgggctg tccgtcggac cactcagtgc attcacaaca
    12661 caactgagaa atgtcccgtt ctgatgatta acctgacgaa tgttgctgag ataccccagg
    12721 cctttcagtg tcagattgaa gtactcagtt ttagcgtttg cagaagaagt atttgcagtc
    12781 ataatgatat ctccattctc agtatcggaa agacagagaa catcatcatc cctgacgggt
    12841 tgtgatattc ccgtcaggga gcaggaacag aactattccg gctgactcag taacagttaa
    12901 agaaaacctt catcgtcatg actgacgata tctgctctca aaggtgggca gatttaccac
    12961 caccagaaac atcctggctg cacatcccct ttcaggttac gggggtgttg atcacccgaa
    13021 ggcgttcctc tcagatcaac ggaacattgg tgcccaccga agcgggatgt ggtcacccga
    13081 aggcgctcct ctcagaccac ttatggagca ttggtggtcg ccgaagcgac atgtagccac
    13141 ccgaaggcgc tcctctcagg ccacttgtgg agcattggtg gtcgccggag cgacgttatg
    13201 tgcccggaca gactgaaatc actgatggaa aaagtcaaca gctaaccaaa aacaaaaaag
    13261 caccggcaag ccggtgcttc agggggatac caaatcccgg tgttgattct catcttctcc
    13321 ggagagagcg actcccggca cggttttcac ggagtgcatg tgccttgtaa ccccgccgtg
    13381 tgcataacgg gttaccggac ctctcagggc tgtcagcccc actgtggtca acagggtgat
    13441 accagaactc gcctgcaatt ttaattcagt ttgtctccga catcaccaga tttttgttta
    13501 tgagaaaaat taatttttct tttcctgccc ctgctgtttc cctgctcacc agcaattccc
    13561 ctgcagtcgg gataacggga gcatccccag aaaaaaccgc tttttccctt ccgtttcacc
    13621 gtcggaccgt tacagagtgg acatgaagga cctgattcca ctgacagatt aaccgtctgg
    13681 ttacgtccgg ataacaccag gtgcctgatc cactgatgct gtttctccat aaacgcctcc
    13741 agtgagattt tcccccgggc aacatcatcc agcgcctgtt cccataaagc cgtcataccc
    13801 ggatttgtca gagagtccgg caatgccgcc accagttctc ttcccagttg cgtggaacgg
    13861 atatgttttc ccgctctctc aatatagttg cgcgcaaata acgtttccag gatccccgcg
    13921 cgtgtggctt ccgttcccag tcccgcattc tccctcagga tctttttcag cgccggatcc
    13981 gttacaccag acgaagcatt cttcatcgca gtaataagac agccttcagt aaaaggacgg
    14041 ggaggtacgg ttttcagctc cttctgttca gcaccatcca gagcacagag ttctcccttt
    14101 atcaaagggg gcaatatgtt tccactttca tcactgtcag gcttttcttc atctgcatga
    14161 ttttccggtg gaaataactg tttccacccg ataatcacat taactcttcc gcgggaacgg
    14221 aataactgcc ccccaatcac aaatgttgcc tcagtaacat cagcttcctg ctccggcaga
    14281 aactgtgcca gatagtgctg acgaatcagt tgatatacct tcagttcatc accgttcagt
    14341 gagctgaggt caaaaacctg ccgggtcgga ataatggcat ggtgagccgt aattttttta
    14401 tcattccaga tacgggatac ccgtcctgat tccagttcag ccagttgtgg aagcacggac
    14461 ggatctgaac gcccgacagc ctcaagcact tcagaactct cctcatgcat cgaggcaggc
    14521 agataaccac agtctgtcct gggataagtt gttgctttgt gagtctcata aagggactgt
    14581 gcgatatcca gaacggtctg agcccccatg ccaaacttcc gggaacaggc ttcctgcaac
    14641 gttcccagcg aaaaacacaa aggtggcggt gttttctccc tcttctgtac aacatcctgt
    14701 acaacagcag aggactgctg acggcacaga ccgagaacag caccggccgc ctgcacattc
    14761 acacaacgtc cctcatcatc gcagtactgc tcagctgcca cccagttact gcggaaacgg
    14821 agcccctcct tttccagcca gacaatcacc tgccaccagg aaaccggcac aaatgtggcg
    14881 atctcctgat cccgtctgac caccattgcc agcgtgggtg tctgcacccg gcccactgac
    14941 aaaaccccag aaattccacg ttcccgtgcc ttcagggtat acaaccggct cagattcatc
    15001 cctgtcagcc agtcagcccg ggcacgcccc ttaccggctt cactgagaag cgcagtctgt
    15061 tcgcccggga gaatattatt cagcgccttc ctgatactgg cctcatccag tgcagataac
    15121 cacagacgcc tgacacgccc tctgtactga cagtactcca gcaactctct ggctatcact
    15181 tctccttcac gatcggcatc tgtggcaata accacttcag aagcgcgttt taacagcccc
    15241 cggatcacaa taagttgttt tttcaccttc ggcttcacca ccatctgcca ttgttccggt
    15301 atcagcggca acacatcaga acgccatggg gaaccgaact gttcaccgta agcttccggc
    15361 tcagccagtt ccagcaggtg tcctatcgcc caggtgacag caatattgtc atctccgatg
    15421 tatccgtcgc cccgcttact gagcgccagt tcacgggcga tatctttcgc ctgagaaggt
    15481 ttttcacaca gaaagagttg catactcacc ccgctttaac cggtggtgaa taaacagaac
    15541 gtttcttacc gctcatcacg tcaggatccg gctcccccat gcggttaacc gcctcctttc
    15601 cacgggctgt gccggacaaa atatcagcac gtgtaacact gactgattta taattttgaa
    15661 taatcccgta aatcctgcgt accgcatacc ctccctgatt caacaactca tcccgttttg
    15721 tgcgggaaat aagtccgtaa tgaaatacct gaaatacctt catgaccagt cgatcatatc
    15781 cgatcagaag ccatacacat cgataaccga ccggagaact actgtacacg ccaatattta
    15841 ccggcgaaga ggatttaata tcagaaaggg agatctgtcc cggcaggttt ttaaggatcc
    15901 cgtcaagcca cgcaagatgc ttttcaattg tttcagttgc cttcgtcagg gcattttcca
    15961 gcctgactaa catcatatct gcataaggat tatccctggc tgaatctctg gttgcctgac
    16021 ccgcaaacgc aatagccctg ggcatactga tgatgtcagg ccgcttttcc tgtttttcct
    16081 ccttctgatt attcctgcgt ccctcccaga gacggatcgc atatcgggta tgaagggcaa
    16141 tagtcagcga tgactgcaga gcaccaactt gttctgattt tctgacttct ccggttttct
    16201 cctggggcat tgtgtttact ccaaatgatt tcatgaccag aagatagtcc gcaggttgcg
    16261 tattaccgac aacaaaaaaa caaatcaggc agaatataca tttaacgccg attatttttt
    16321 aatcgctgtt gacactggca acaccgctca ctttttaacc tctgttgcca ctggcagcac
    16381 tgttcgcttt ttaacctctg ctgccactgg cagcactgtt cactttttaa cctctgctgc
    16441 cactggcagc actgttcgct ttttaacctc tgctgccact ggcagcactg ttcgcttttt
    16501 aacctctgct gccactggca gcactgctca ttttttaatc tctgctgcca ctggcagcac
    16561 tgttcacttt ttaacctctt ctgccactgg cagcactgtt cgctttttaa cctctgctgc
    16621 cactggcagc actgttcact ttttgacctc tgctgccact ggcagcactg ctcatttttt
    16681 aatccctgct gccactggca g
//
LOCUS       DAZKJR010000114        16175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKJR010000114 DAZKJR010000000
VERSION     DAZKJR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.115"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            395..1294
                     /gene="yegS"
                     /locus_tag="ACHOWS_003757"
     CDS             395..1294
                     /gene="yegS"
                     /locus_tag="ACHOWS_003757"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFQ0049271.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1376..2149)
                     /locus_tag="ACHOWS_003758"
     CDS             complement(1376..2149)
                     /locus_tag="ACHOWS_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0049272.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2255..3295)
                     /gene="gatD"
                     /locus_tag="ACHOWS_003759"
     CDS             complement(2255..3295)
                     /gene="gatD"
                     /locus_tag="ACHOWS_003759"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0049273.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDVVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3343..4698)
                     /locus_tag="ACHOWS_003760"
     CDS             complement(3343..4698)
                     /locus_tag="ACHOWS_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFQ0049274.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(4702..4986)
                     /gene="gatB"
                     /locus_tag="ACHOWS_003761"
     CDS             complement(4702..4986)
                     /gene="gatB"
                     /locus_tag="ACHOWS_003761"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFQ0049275.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(5017..5469)
                     /gene="gatA"
                     /locus_tag="ACHOWS_003762"
     CDS             complement(5017..5469)
                     /gene="gatA"
                     /locus_tag="ACHOWS_003762"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFQ0049276.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5479..6741)
                     /gene="gatZ"
                     /locus_tag="ACHOWS_003763"
     CDS             complement(5479..6741)
                     /gene="gatZ"
                     /locus_tag="ACHOWS_003763"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFQ0049277.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6770..7624)
                     /gene="gatY"
                     /locus_tag="ACHOWS_003764"
     CDS             complement(6770..7624)
                     /gene="gatY"
                     /locus_tag="ACHOWS_003764"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFQ0049278.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7853..8905)
                     /gene="fbaB"
                     /locus_tag="ACHOWS_003765"
     CDS             complement(7853..8905)
                     /gene="fbaB"
                     /locus_tag="ACHOWS_003765"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFQ0049279.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9162..10439
                     /locus_tag="ACHOWS_003766"
     CDS             9162..10439
                     /locus_tag="ACHOWS_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFQ0049280.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10436..11440
                     /locus_tag="ACHOWS_003767"
     CDS             10436..11440
                     /locus_tag="ACHOWS_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0049281.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAYFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            11437..12402
                     /locus_tag="ACHOWS_003768"
     CDS             11437..12402
                     /locus_tag="ACHOWS_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFQ0049282.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAATLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12376..13122)
                     /locus_tag="ACHOWS_003769"
     CDS             complement(12376..13122)
                     /locus_tag="ACHOWS_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0049283.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13174..13992)
                     /locus_tag="ACHOWS_003770"
     CDS             complement(13174..13992)
                     /locus_tag="ACHOWS_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFQ0049284.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(14057..14857)
                     /gene="thiD"
                     /locus_tag="ACHOWS_003771"
     CDS             complement(14057..14857)
                     /gene="thiD"
                     /locus_tag="ACHOWS_003771"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0049285.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWTDTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14854..15642)
                     /gene="thiM"
                     /locus_tag="ACHOWS_003772"
     CDS             complement(14854..15642)
                     /gene="thiM"
                     /locus_tag="ACHOWS_003772"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFQ0049286.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15677..15773)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15865..16137)
                     /gene="rcnR"
                     /locus_tag="ACHOWS_003773"
     CDS             complement(15865..16137)
                     /gene="rcnR"
                     /locus_tag="ACHOWS_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFQ0049287.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         3978 a         4212 c         3898 g         4087 t
ORIGIN      
        1 accaacgaca cgctctggtg tttaaatata ataatggcat gattattata attgaatagg
       61 attataataa atgttctgta caacatttcc tacataagta ggaattacat acacggatgc
      121 ccttccaggt aaccccatgg gggggttaat atatttttaa tagtatattg aagatgccac
      181 tgtttagttg aatattaggt atatgttctt ttttgaaatt taccggtggc taccgttaat
      241 attcgctgtt cccattgcaa gctcctggtg gtaaccactg aatcctccat acttgaactg
      301 actttttatc ctccgacttt catcctgttc tgactccacc ttttcttttc tgctctacac
      361 tatctacagt ccaatcataa aggcacatac gatcatggca gaatttcccg ccagcttact
      421 gattcttaat ggcaaaagta ctgacaatct acccttgcgc gaagcaatta tgctgttgcg
      481 tgaggaagga atgacgatcc atgtgcgggt cacctgggag aaaggcgatg ccgcacgata
      541 tgtagaggag gcacggaagt tgggcgtcgc aacggtgatt gccggtggtg gcgatggcac
      601 cattaatgaa gtttctacgg cgttgattca gtgtgagggg gatgacatac ccgcgctggg
      661 aattttacca ttaggaaccg ccaatgattt tgccaccagt gtagggattc ctgaggcact
      721 ggataaggcg ctgaaactgg caattgccgg taacgccatt gcgatagata tggcgcaggt
      781 caataaacaa acctgtttta ttaatatggc gacaggcggg tttgggacgc gtattaccac
      841 agaaacgccg gaaaaattaa aagccgcgct gggtggcgtc tcttacatca ttcatggctt
      901 aatgcgcatg gacactctgc aaccggaccg ttgtgaaatc cgcggtgaaa actttcactg
      961 gcaaggtgac gccctggtca ttggtattgg taacgggcgt caggccggtg gcggtcaaca
     1021 attgtgcccg aacgcgttaa ttaacgatgg cttgctgcaa ctgcgcattt ttaccggcga
     1081 tgaaattatt ccgactctcg tatcaacctt aaaatctgac gaagataacc cgaatattat
     1141 cgaaggagct tcgtcgtggt ttgatataca agccccacac gaaatcactt ttaatcttga
     1201 tggcgaaccg ttgagtgggc aaaacttcca tattgaaata cttccggcgg cgttgcgttg
     1261 tcgattacca ccagattgtc cattgttgcg ttaatcaaac tattctctgt tgaaccagag
     1321 agaatccgta atgccggatg cggtgtaaac accttatccg gcatacagaa caatactaca
     1381 ccgtaattaa ttttacccgt gtcttctgca atgccagttt atccacctca gacacagcat
     1441 catcggtaat aataatgtca attttttcga ttggcagtac ctgattaaaa ccgcgacgat
     1501 taaatttcga tgaatcgaga acggcaatca ctttattcgc cgctgtcacc atcgcaccgc
     1561 taacggaata accttcatta aaggtcgtaa taccattaac cgcatcaata ccatcagcac
     1621 cgacaaacat taaatcggca ttaatatcct gcaatgaacg ctcagcaatg gaaccatgca
     1681 tcgagcgcgt tttatggcgc accgtaccac cacaaaccac caaagtgatg tctttatttt
     1741 cggaaagggc aaacgcagcc gggagactgt tggtaatcac cgtgatattt ttggcggtca
     1801 ttaatccttc ggcgataagc attgtcgtac ttccgctgtc gagaataacg gtcatccctt
     1861 cgtggatcat tttgactgcc gcctgcgcta ttctgttttt cggcgcgctg gcgagctgaa
     1921 atcgctcttt aaacccgact tcctgggtct cggtttcgct attaccggac attattttcg
     1981 ccgcaccgcc atgaaagcgc gtaaccacgc ctttttgttc gagaaagcgc aaatcggcac
     2041 ggattgtcgc ttccgaggca gcaaatactc ccgccagatc ctgaacaagc acggttccct
     2101 gttcattcac taattggatg atcttattcc ttcgctcgaa tgaattcatc gtcatttacc
     2161 catattcaat tgtggctagt gtaaacgaag ggggaccatt ggtgaatgat ctgttttgca
     2221 caataccggg cctgcatcac gctggcccgc ggtttcaggg aatgagcaac actttgcccg
     2281 gcatggcatt acgagcgatg tcacgcaccg cctgggcgaa gctttcaaag cttccacggt
     2341 gagcgattaa tggctccagg cttaacttac gttctgtcag caaccggctc gccgtttccc
     2401 actcctgccc cggccaaggg ctggagtagt tcatccaact gccgataacc gtcagctctt
     2461 tacgcaatat tttgccaaac gttgcagatg ttaaatgcag atcctgatgc aacgtgccca
     2521 ccagcgccag ttgggcatga ggaccggcaa tctctaccgc cagttcgacg gtttgcggga
     2581 cgccagccgt ctcgaggata agctgattaa agcgcagctc gcgtaaaacg ccctgtattt
     2641 gcggcgcgct catttcacgg ctgttaaatg tttgcatcgc tccgaaagat tttgccagtg
     2701 ccagtttttc tgagctaatg tcgatagccg tcacactctt tgctcccagc gcgacagcgc
     2761 actgaattgc cagcaggcca atggttccgg caccaataat aataacgttt ttattctcac
     2821 atccttgcgc taaatggaac gcatgcaggc caacggtaat cggttcaata aaagcgccat
     2881 cctcaatagg catatccgca ggtagtgcaa agacattgtt tcgcttaacg acaatatatt
     2941 cggcaaatcc accatcacgc cgcgagccaa taaaatcata ttttgcgcac tgggaataaa
     3001 accctttcaa acactctgga caagtaaaac agggtaataa cggcacacag gcaaccacat
     3061 cgccagggtg taaatcatca acaccggatc ccaccgcatc aatatagccg ctaaattcat
     3121 gacctaacgt tattggataa taatgtgcac catttttaaa tatcctgggt aaatcggaac
     3181 cacataagcc cgagctggca atttttaccc gcacctcatc ctgatgttta atttcaggaa
     3241 tgacgctttc tgcaacgcgc acgataccat cagtatcatt caccactgat ttcataaaaa
     3301 ctcctgatta ttaaggggga taacctcccc ctcaggtaaa aattattctg cgagaacggc
     3361 tttctcttgt ttaataaagc cacgcgctct acgccaggtc atgaaaatac cggtaagata
     3421 aatggcaccg ataataatga aaccgggaat attttgcggg gagaaaacct gaatcagtaa
     3481 ccaggtaatg ggagaaccgc cctgatccat tgaagccacc atgcccccgg cttttaacgc
     3541 cccagcatta gccgccagtt gggtgtgcaa accaatagtt tgcgtcgcga tccacagggt
     3601 gatgctcata atgatgacac ccgagattaa ggtgcggaac agatttccac gatgcaccgc
     3661 gaccgccatc gccacaaaga agccgatggt ggcaaggtcg ccaaacggca gcacctgatt
     3721 acccggcaca caaacagcaa ttaaaatggt gagtggaata aaaatcaggc ttgccgatac
     3781 caccgccgta tgtcccagca gcaacgccgg atcaaggcca atcaggaact cctgaccgcc
     3841 gaacttcgcc tgtaaacgac tacgggcctg cttagcgatg ggcgttaaac catccatgat
     3901 gggtttaatc acccgtggca tcagcagcat aactgccgcc gtttttaccg ccagctgcaa
     3961 tacacctttg acatcgtaac ccgcgaggat gccgataatc agccccatca caaaacccac
     4021 ggtgaccggt tcgccaaaag ggccaaattt gcgctgaata tcatcggcgc taaatttaat
     4081 tcggttaacg cctgggattt tctcgatgat agcatcgacc agcaccgcaa tcggccccat
     4141 atacgccgac gtaccgtgcg gaatagcaat gccttccagc tcaaagaaat ttcgggtatc
     4201 gcgggcaaac cagtcgccga gcttataaac aaacgccgcg tgaattacca cacccgccat
     4261 ccctatcatc catgaaccgg ttgccagatg cagcaacgcg ccggtgaagg tcatatgcca
     4321 gatattccag atatcaacat ttaccacccg cgtcatacgg gtcagtaaca tcgccacgtt
     4381 aaccagaatc gcaatcggaa tcgccaccag cgcaatttgc gacgcccagg tcatgggtga
     4441 agagcccggc cagccgacat cgaccacatg cagattcagg tcgaaatttt ccgccatcgc
     4501 tttcgccgcc gggccaatgg aatccagcat taagccaatc acaaggccaa taccaacaaa
     4561 gccaatcccg atatgcagac ctgctttaaa gcaatcgcct gccttcatgc ctaatatttt
     4621 agaaaaaata atgatgacaa tcggcagcat caccgtaggg ccgaggtcga gaatataacg
     4681 catgacttct gaaaacatag gtcacccctg taagatagtc agaattttat tttgtaatgc
     4741 ttcgataccg acaccagaaa caaaaggcat gccgtgaact aacggaatat cgccaaaact
     4801 tcgatccacc ctggcagtgg tgcatatcaa atgcacgcca tccatatagg tttctatttc
     4861 attaacccga cactggatta attcaacagg aatattatga ctctgacaca actctttaat
     4921 ttcttccgcc gccatcgtag aggtcgcaac cgcgcctccg caagcgacaa taatcttgcg
     4981 tttcataaat agtccttttt attgttacag agggatttat tcgtctgaat ctaaaacata
     5041 ctttgtgaag tattccttta actgggtttc aggaagagtg attagtgtct cgacgatctc
     5101 gggctgttgt aacttgccaa ataaacagcg taaaagtttc aattgctgct gcggattttc
     5161 cacaattaac gcaatcacca acgataccgc cacgtcgtta tcatcatccg cttgctgaaa
     5221 atgaacttta tttgttggcc ttaacagata aatggctgac gacttagcat gaatcgcctc
     5281 acaatgcggt attgcaatgg cgtgctgctc aagcattatc ccggtaggga attctgcttc
     5341 tctggcaatt aatgcctgtg gccaggtatc atgaaccaca cctttcgcga gcatctcatt
     5401 accgatatgg gttaaaactt cgctacgatc gacaaaagaa attccgctac gaacaaacag
     5461 gttagtcata taccgtcctt attccgcaca gccgtagcga taggcgcgca aaacatcata
     5521 aattttatcc ataatcaact gatgcggtat tgctgataat tccccggact gaatgcgttc
     5581 aaattgttta ggaagatact gactaatcat gcccagaggg atttccatgc cttgcagatt
     5641 caccatcatc gtttcgacgc tatttttaat ccggctatgc ggccagtaat aacgaatacg
     5701 atccgacagg ctgtaacgaa tatccagtaa tgaatcgtta aaacccgtac gataatattt
     5761 tttccagtat tgcggttcgt caaacatcac ttcttcaatt accgccaggc aaccgctgcg
     5821 attttctggg gcgataagtt cctgctcaat ttgcgccagc gcaaatatcg cctcacgtaa
     5881 agcaaaggtt aatgccggac cgactttcaa aattgcaaaa tgatcgcgaa ctaattcccg
     5941 ataggccgtc tgagtctggt aatcggtgga atgcgcttca taaaccattt tggttttttc
     6001 tatccattgc gccagcgcct gagcttcttg cgcctgataa tgaataatat tgctatggtc
     6061 aaattccaca cccggctgca ccacgatggc aatcacgcgt gttaacgcct ctgccagacc
     6121 gcgggcaata aacgcttttt gatgcgtgcg taaagtattg gcggcatctt caacctgggt
     6181 gatatgtact gactgaatgg cgctggcctc accgcccgga accggaactt cggtgccaat
     6241 gacatagctc agttgctcac gctggcaatc tgtcgccaca ctttccgcag caaagcaaag
     6301 cacagcagct cgttccgcaa ccgtttctgg ggctaacggt ataggatccc ccgcgcaaga
     6361 cattgacgca tcaagatgaa ttttactgaa gccggcacga acatatgcct ttaccagttc
     6421 gacggatttt tccatcgccg catccacatt ttcttgctgc cagcagtttg gtcccagatg
     6481 gtcgccaccg agaataatac gctcacgagc aaatccgact ttatcggcaa tcgcaaaaac
     6541 aaattcgcga aagtctgccg gtgtcattcc ggtataaccg ccaaattgat tgacctggtt
     6601 tgacgttgct tcaatcagca ctttacgcgt gctgttgcga tcaaatgcca gcgccgcttc
     6661 gataaccaac ggatgtgcag aacagactga acatatgcca atatgttcac cagctttatg
     6721 ccgggcaatt aacgttttca tgttttttcc ttgttaaatg gcaggtgcgt tatgccctgc
     6781 cctcgcagcc acaatcggca atcactttgc tcaccacatc gcgcattgcg gatttagccg
     6841 actgcaaata atcccgggga tcggtcgctt cggggtgctc tgtcaggtaa ttttttaacg
     6901 cctgcgagaa ggcatttttt agctctgttg caacgttgat tttgcatatc cccagtttga
     6961 tggtttgctg aatatcctta gttgataatc ctgacgcgcc atgcagcacc agcggtaagt
     7021 tcacccactg gcgaatgttc tccagtcgag aaaaatcaag cgccggtgcg ctggcataca
     7081 taccgtgagc cgtgccgatg gcgaccgcca gggaatcaat tccggttgcc tcggcaaatt
     7141 cacgcgcctg aacagggttg gtgtacaacg catcggcttc attgacttgc acatcatctt
     7201 cctggccgcc aagttgcccc agctccgctt cgacgctgac atcaaagcga tggcaaaaat
     7261 ccaccacctc tttgacccgt gaaatatttt gcgcaaaagg caaatgcgag gcgtcaatca
     7321 tgactgagcg cacgccagaa cgaaccttct gagcgatatc gtcaaatttc gtgtgatggt
     7381 cgagatgaat tgccagtgga tggtgatatt gcttcgccat cgcgctgacc agcgccaaca
     7441 gattttctgt accggcatga gtaaatgtgc caggcgttcc ggcgatgatg accggcgcat
     7501 gcaggttggc agcggtttct accaccactt gcatcgtttc gagattgtga atattgaatg
     7561 ccggaaccgc ataaccgccg cgctgtgcgt tgttcagcat ctgctttgtc gataccacgt
     7621 acattttcat atcctgtcgt ttgttttcga tttcaaaata taatgaaatg atttgttttt
     7681 aaatgtcgcg ataacgatca caaaaacgac aatatgaaaa tcaatccagg aatgaaaggc
     7741 aaaaaaaacg gcctcccggg ggaagccgta acaaagtacg cgtggtttta tgccggatgt
     7801 ggcgttaacg ccttttccgg cctacggggt agtgctgttc gtatgaagcg catcaggcga
     7861 tagtaatttt gctatcgaga taaacgtcct gcacggcgtt aatcagtttc acgccgtcag
     7921 ccatcgattt cttgaacgcc ttacgtccaa gaatcagccc cattccgcct gcgcgtttgt
     7981 tgataaccgc agtacgcact gcatcgctga ggtcagtttc accgcctgca gcaccgccgg
     8041 agtttatcaa cccggcccgg cccatatagc agttagctaa ctgataacgc accaggtcaa
     8101 tcgggttttc actggtcagt ttgctgtaga ctcgatcgtc ggtataaccg taattaatcg
     8161 ctttatagcc gccgttattt tccgccattt tttgtttgac gatatctgca cctatggttg
     8221 ccgccagatg gttcgcctga ccagtcaggt cggcggaaac atggtaatca acgccatctt
     8281 tcttaaaggc ggagttacgc aaataggccc acagcactgt caccatgccc agctcgtgcg
     8341 cacgttcaaa agccgcagaa atttcttcaa tctggcgacg tgactcttcc gagccaaaat
     8401 agatagtcgc accaaccgcc accgcgccca tattgaacgc ctgctccacg ctggcataca
     8461 gcgtttgatc gtaggtattc gggtaactta gcgtctcgtt gtgattaagt ttgacgagga
     8521 atggaatgcg atgcgcgtaa cgccgcgata ccgatgccag cacgccgtaa gttgacgcca
     8581 cacagttaca gcccgcttcg atcgccagtt caacaatgtt tttcgggtca aagtagagcg
     8641 ggttagcggc aaatgaagct ccggcagagt gctcaacgcc ctggtcaacc ggcagaatag
     8701 aaagatatcc tgtgccagcc agacgcccgg tgttgtacaa cgtctgcata ttacgtaaca
     8761 ccgctggcgg gcgattattg tcaatcatca cgcggtctac gtagtcatgt ccggggagat
     8821 aaagctggtc agaaggaata gtcatacaac ggtgctgtaa aaggttgtcg gcgtctttgc
     8881 caagcaactg cgcaatatct gtcattacta tgctcccgta aattccgatt ggatatcggc
     8941 tatggatttt cctggcccgc cttttgcggg caatcataat cctggtcgtt acaggcgagt
     9001 ttttccatct tttggatgtt ttttcagcgt tttctgttgg cacgattcat cagaaaaaat
     9061 gttatcgcgg tcaagttttc accgcaaagg tatttaaaag gtattattaa atggtattgt
     9121 catcgcgtac cttacattac ctgtcatgaa ggaattaaaa gatgaaaaca acagcaaagc
     9181 tgtcgttcat gatgtttgtt gaatggttta tctggggcgc gtggtttgtg ccattgtggt
     9241 tgtggttaag taaaagcggt tttagtgccg gagaaattgg ctggtcgtat gcctgtaccg
     9301 ccattgcggc gatcctgtcg ccaattctgg ttggctccat cactgatcgc tttttctcgg
     9361 cgcagaaagt gctggcggta ttgatgttcg ctggtgcggc gctgatgtat ttcgctgcgc
     9421 aacagaccac ttttgccggg ttcttcccgt tactgctggc ctactcgcta acctatatgc
     9481 cgaccattgc gctgactaac agtatcgctt ttgccaacgt gccggatgtg gagcgtgatt
     9541 tcccacgcat tcgtgtgatg ggcactatcg gctggattgc ctccggtctg gcatgtggat
     9601 ttttgccgca aatgctgggt tatgccgata tctctccgac taacatcccg ctgctgatta
     9661 ccgccggaag ttctgctctg ctcggtgtgt ttgcgttttt cctgcccgac acgccgccaa
     9721 aaagcaccgg caaaatggac attaaagtca tgctcggcct ggatgcgctg atcctgctgc
     9781 gcgataaaaa cttcctcgtc tttttcttct gttcattcct gtttgcgatg ccactggcgt
     9841 tctattacat ctttgccaac ggttatctga cagaagtcgg catgaaaaac gccaccggct
     9901 ggatgacgct cggccagttc tctgaaatct tctttatgct ggcattgccg tttttcacta
     9961 aacgctttgg tatcaaaaag gtattgttgc ttggtctggt caccgctgcg atccgctatg
    10021 gcttctttat ttacggtagt gcggatgaat atttcaccta cgcgttactg ttcctcggca
    10081 ttttgctgca cggcgtcagt tacgattttt actacgttac cgcttacatc tatgtcgata
    10141 aaaaagcccc cgtgcatatg cgtaccgccg cgcaggggct gatcacgctc tgctgccagg
    10201 gcttcggcag tttgctcggc tatcgtcttg gcggtgtgat gatggaaaag atgttcgctt
    10261 atcaggaacc ggtaaacgga ctgactttca actggtccgg gatgtggact ttcggcgcgg
    10321 tgatgattgc cattatcgcc gtgctgttca tgattttttt ccgcgaatcc gacaacgaaa
    10381 ttacggctat caaggtcgat gatcgcgata ttgcgttgac acaaggggaa gttaaatgaa
    10441 aacagaacgt attctcggtg ctctttatgg gcaggcgtta ggggatgcga tggggatgcc
    10501 ctccgagctt tggccgcgca gtcgcgtcaa agcgtacttt ggctggattg accgttttct
    10561 gcctgggcca aaggagaata acgcggcctg ttattttaac cgcgccgaat tcaccgacga
    10621 tacctcgatg gcgctgtgtc tggcggatgc gttactggaa cgtgaaggca agatcgatcc
    10681 ggatctgatt gggcgtaata ttctcgactg ggcgctgcgt ttcgacgcct ttaacaaaaa
    10741 cgtactaggt ccgacctcga agattgctct caacgccatt cgcgacggta aacccgttgc
    10801 tgaactggaa aacaacggcg tgaccaacgg cgcagcgatg cgcgtctcgc cattaggttg
    10861 tttgcttccg gcgcgtgatg ttgattcctt tattgatgat gtggcgctgg cctccagccc
    10921 gacacataaa tccgatctgg cggttgcggg ggcggtagtc atcgcatggg cgatttctcg
    10981 tgccattgac ggagaaagct ggtcagcgat tgttgattca ctgccttcaa ttgcgcgaca
    11041 tgctcaacaa aaacgcatca ctaccttcag cgcctcactg gcagcacgtc tggagattgc
    11101 gctgaaaatt gtgcgcaatg ccgacggcac cgaatccgcc agcgaacagc tttaccaggt
    11161 cgttggcgca ggtaccagca ctattgagtc cgttccgtgc gccattgcgc tggttgaact
    11221 ggcacaaacc gacccgaacc gttgcgccgt cctgtgcgct aaccttggcg gcgacacaga
    11281 caccatcggt gctatggcga cggcaatttg tggcgcgttg catggcgtta acgctatcga
    11341 tcctgcgtta aaggcggaac tggatgcagt aaatcagctt gatttcaacc gctatgccac
    11401 ggcgctggcg aagtatcgcc aacaacggga ggcgatatga gcggcgctcg attacacacg
    11461 ctgctgcctg aattagccac gcgtcagcca gtgatggtcg tcggcgcggc ggtcattgat
    11521 gtgatcgccg acgcttatgc cctcccctgg cgtgggtgcg atatcgaact gaaacagcag
    11581 agcgttaacg ttggcggctg cgcactgaat attgccgtgg cgttaaagcg cctcggcatc
    11641 gaagcgggta atgccttgcc gctcggtcag ggcgtgtggg cggagattat tcgcaaccgg
    11701 atggcaaaag agggcttaat cagcctgatc gataacgccg aaggtgataa cggctggtgt
    11761 ctggcgctgg ttgagccgga tggcgaacgc acttttatgt cattcagcgg tgtggaaaat
    11821 cagtggaatc gccagtggct ggcgcgatta accgttgcgc ctggcagcct gctctatttt
    11881 tccggttatc aactggcctc gccctgcggc gaattgttag tggaatggct ggaagagctg
    11941 caagacgtga cgccatttat cgattttggc ccacgtattg gcgatatccc ggatgcatta
    12001 ctggcgcgga tcatggcctg tcgaccttta gtgtcactca atcgtcagga ggctgagatt
    12061 gccgccgaac gttttgcttt atccgcagag ataacaacac ttggcaagca atggcaggag
    12121 aaatttgctg cgacgttgat cgttcgtctc gataaagagg gcgcatggta tttcagcaac
    12181 gacgcttctg gctgcattcc ggcatttcca acgcaagttg tagacaccat tggggcgggc
    12241 gacagtcatg ccggcggcgt acttgccggg ctggcctctg gtctgccact ggcggatgcc
    12301 gtattactgg gcaatgcagt ggcgtcgtgg gttgtcgggc atcggggcgg tgattgtgcg
    12361 ccaacgcgcg aggaactact cctcgcacac aaaaacgtat agatcgctgc gacagtggct
    12421 gatgctgtac tcaataggcc gttgctgttg gtcaagcgcc acttgcttga tcaccagcac
    12481 cggtattttg ctatctaact gaatatgtga ctgaaactcg gcatccggca tccgggcgct
    12541 aacgcgggaa cgcgtacgtt gtgggtaaat gtgctggctg cggaagtagt cataaagaga
    12601 aatcccgatg gcatcaacat catgaattaa atgtgccgga acccacgatt cctcaatcga
    12661 taccgcctct tcatcgacat agcgaatacg cttaagcaaa aagacatcac ttcctgcttc
    12721 gaccgctagc tgctgcgcga cttcttcggg acatttcacg acacgcttgt taacccatag
    12781 cgtatcgggc tttttcccgc gcaataccac ctgctgagaa aaacctcgcg cttctttcaa
    12841 cgagtattcg aagatgttgt tgatctgcgt gccgtaacca cgcgaacgcg tcaccacgcc
    12901 ctcttcttcc agcgcctgca tcgccttgcg caccgtaatg cgcgaaacgc cggttaactg
    12961 acttaaatca cgctcaccgg gcaaaatatt gccatgctcc agcaccccgc tgcgcacggc
    13021 attttttacc gtttcggcaa acttaatata aagcggcgtg ttatccgccg ctaaaatacg
    13081 ttcgttcagt tgggcaatca gctgggtatg cgcttgttcc atttgtcttt ttcctgccag
    13141 ggagtctggt gggagtatat cactgcttct tatttaaggc gtttctttaa tcccatcaac
    13201 gaatccctgc aactcctcca ccgtgccatt aaacacatta aaatccaccg gaccattaat
    13261 gccatctacc tgtccacggt cggaatgctg ccagaagcgc caggccattc cgtcattgtc
    13321 cggacgacgt tgatagtagt gagccaccca ccacggatat tcattaaaat agcccgccag
    13381 attggtgtga taaaaaacgg ctcctgagta aataattggc ttttttcccg tacgtttttc
    13441 gaccattttt agccactgac ttacccgctt gcgtaattct tttgccgata attttcctcg
    13501 ctcttctacg tccagtaccg cagggaaatc gccttgtgag aaatccaccg tttgcagaaa
    13561 taatcgcgcc tgaactgaag cggatactga cggggagaaa taatgatacg ccccgcgcag
    13621 caggccattt tcgcggctta gttgccagtt acgcgaaaaa taggggtcca ccagcttttc
    13681 gccttccgtc gccttaataa aagcaaactg taagcggata ccgttgtcgc gcatttttgc
    13741 cacccgctgc cagtcgatcc gctcctgcca gcgcgaaaca tctattccgt gaatggtgta
    13801 actggcagga atgcgaatag cgaaagattt aaccgggcga taaccgtaga agtggatgta
    13861 gtctttaatc tgaattgctg tcgaatagaa aaaattcacc gtctgacgcg gataaattgc
    13921 aacgatggaa attagcccaa gagcgcataa taaagcggtg aatttttttc gactggtgat
    13981 tcttagctgc attggtttca tccctgaatg tcagcgccaa aggctgacaa taaccaaagc
    14041 agctatagta cggtgctcac caccaggcgt ggaagtggtg aaccggacca ataccgtgac
    14101 caacttccag cgtgtcggcc tgggctaatg ccgatgaaag ccagcttttt gcctcctgta
    14161 cggtgtcagt ccagtttgta tggcgcgggc gcagtgcagc caacgccgca gagagcgtac
    14221 aaccagtgcc gtgagtgttt ttggtcataa tgcgctgtgc ggtaaaccgt tgttcaccct
    14281 cgcgggtaaa cagccagtcc gggctttgct catcatccag atgaccacct ttcattagca
    14341 ctgcgccaca gcccatcgcc aacagcgatc gcccttgttc cagcatttcc tgttcggtgc
    14401 gcgcgtgtgg cgcgtcgagc aaggcggctg cttcgggcaa gtttggcgtt attaatgaaa
    14461 cctgtggcaa taatcgactg cgcagcgtag caaccgccga aggtgaaagc agcgggtcgc
    14521 cgctttttgc cagcataacg gtgtcgagta ccacgttttg gatctgataa cgttgcaacc
    14581 gttctgccac cgcttcaaca atatcggttt ccgccaacat accgatttta gtggtgtcga
    14641 ttcgcacatc gctgaacacc gaatcgagct gggcggcgac aaaatcaggc tcaatgcgat
    14701 acaccgactg tacaccacgg gtattttgcg ccaccagtgc agtaataact gagcaaccat
    14761 aagcgccaag tgccgagaag gttttaagat cggcctgaat ccccgcacca ccactcggat
    14821 cagtaccggc aatcgtcaga gcgttaattc gtttcacgct tgcacctcct gcgtcaattg
    14881 ccagagcgca tcaaggaaat gtggaacaaa actgcctggc ccctcgctac tggcgactgc
    14941 gcgttctccg gcttgtttca tccagtgaca ggcagatgcg atattttcca gcgtatcgcc
    15001 tggtagcgca caacaggcag cgacaaccgc cgataatgca cagccagttc ctaccacttt
    15061 ggtcattaac ggatcgccac cgtgaatgcc aatgatacga tgtccatcgg taacataatc
    15121 cacctcgcca gtgaccacga cgattgcgcc agtttcccgt gccagtgttt gtgcagcggg
    15181 tatcgcgtta gctgcggcat cagtggtatc cactccccgt ccaccattag caacgccagc
    15241 taatgccatg atttccgaag cattaccacg tatggcggtt ggcttatggg acagaagttc
    15301 cacacaaaaa cggcggcgat aatcgagcgc accaaccgca accggatcaa gcgtccaggg
    15361 cgtttgtgat cgtgttgctt gctcaacggc agcactcatc gcttgcgctc gtggctgcgt
    15421 cagcgtgcca acgttaatca acaaggcact ggcgatagcc gcaaactgac tggcctcttc
    15481 ggtttcgata accatcgctg gcgatgcacc gatcgccagc aaggtattgg cagtaaaagt
    15541 ttgcaccaca tcgttggtca tgcagtgcac aagaggggaa tgttggtgaa aaaggtgtaa
    15601 cgtgtgcgca gattgcgcgg aactcagcag gtcgacttgc atagtttgct cctgccataa
    15661 cgtgaagaag caatgacctg gtggtccgtg acttccctac gctggcatta tccagatcag
    15721 gtgatacggg tatttctcag ccttcacaaa gaagggcacc ccgagtcgtt tggttgcgat
    15781 aataaatcgc agaggaggat ggtaatgtcc agcgcacgcg ttgtaaacgg ggaaaaacgg
    15841 cggtattaca atcgcgttaa taaattattt gatataagaa tccagcacct tcagaacgac
    15901 atccagatct tcttcacgtt ttagctcatc cccctggtga acgatgtgtt ccgtcagatg
    15961 gcctttaatc acttcccgca tcagaccgtt taccgcgcca cggatagcag caatctgttg
    16021 taaaactgca gcgcattcat gcggttcgtc gagcattttc ttgagcgcca cgacctggcc
    16081 ctgaatctta ctggcacgcg ctttcagttt ctgtttatca cggattgtat gagacatggc
    16141 aacacctggt taacaagaat atgaaaaatc atagc
//
LOCUS       DAZKJR010000115        16112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKJR010000115 DAZKJR010000000
VERSION     DAZKJR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.116"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(163..2649)
                     /locus_tag="ACHOWS_003774"
     CDS             complement(163..2649)
                     /locus_tag="ACHOWS_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFQ0049288.1"
                     /translation="MSIPALNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARY
                     EVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPAFIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAVILLSAPKGV
                     AIASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGTK
                     KEGVTDDEGCTETFFSDKEEEIEIEILLGV"
     gene            complement(2697..3314)
                     /locus_tag="ACHOWS_003775"
     CDS             complement(2697..3314)
                     /locus_tag="ACHOWS_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049289.1"
                     /translation="MKTNWIEHMKNLLVKKPEVKDFFSKDKFPLNLVDGSYPIFISLK
                     DRGIYAAHSIFICSDDELHAIKEHLSNIFNVNKDTIGNEGVIYDSGIVHHPLFRDAIE
                     IQKTYSLIYTFTKSFKGNLIDLVTNSSFFVETIKSAGIKEPVPWDVFPELEPDTFGSL
                     QGELEFWWESIWSPFWHSLSPDERNDFLVKNNATPQWREFIEFHC"
     gene            complement(3304..4194)
                     /locus_tag="ACHOWS_003776"
     CDS             complement(3304..4194)
                     /locus_tag="ACHOWS_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFQ0049290.1"
                     /translation="MVISENTVGTNDIPDSNHDILGPLMPARGFFFIVIDEPGKDGFL
                     VRKIYASPNSPYLTKMNLDPLRLLSETEPERYGLMPKDYSAPTTVAEHVMGNNKSSLI
                     STSSIFPDGSPRFEGKTIFIDVHKAKNAGLKLISTAEIITALEEYKKQYPHLAKRVEK
                     ISGYVDDIDKEVLIEGRKVPAQTIFTPESLKTTQNIVKAGRVVQVFSIFFTAYDLEQA
                     TEKSFETKSIKPIGAEAIRQIGGWGGAVAGAKIGTVIGASLGVTTGPGAVATGIIGGI
                     VFGAAGYFGADWVADYIDEN"
     gene            complement(4188..6677)
                     /locus_tag="ACHOWS_003777"
     CDS             complement(4188..6677)
                     /locus_tag="ACHOWS_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFQ0049291.1"
                     /translation="MGIQNFDHSHHKLKIRGLKSPVDVLTFTGREQLSAPFRYDIEFT
                     STDKTIEPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAP
                     AMAILNGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAI
                     ASGEHLQLAAAKNVIANAGNNADVGAVKNMFIGVGQALSVFVRKAGIKLFANKGAISV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTQGEYNV
                     KAGYYGRKPKAKLTPELMAFPVISAKFDEQVRVIDDDGKFIANVPYYIEDSIGNIYKG
                     FTDSAGCCERIFTEEADNLNVLLGIKALEKW"
     gene            complement(6689..9334)
                     /gene="tssH"
                     /locus_tag="ACHOWS_003778"
                     /gene_synonym="clpV"
     CDS             complement(6689..9334)
                     /gene="tssH"
                     /locus_tag="ACHOWS_003778"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFQ0049292.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLHPLLDAQSDECPA
                     RQPQVTEPLTSAQPESATTDVPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAAQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     VQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQRPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            complement(9526..10017)
                     /locus_tag="ACHOWS_003779"
     CDS             complement(9526..10017)
                     /locus_tag="ACHOWS_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFQ0049293.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVPTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            complement(10075..11727)
                     /locus_tag="ACHOWS_003780"
     CDS             complement(10075..11727)
                     /locus_tag="ACHOWS_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFQ0049294.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDTAVLTGQLRRFAHSMAIVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRTIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPLGRAVNRRVEISLVPRSDACQDVK"
     gene            complement(11740..12405)
                     /gene="tssL"
                     /locus_tag="ACHOWS_003781"
     CDS             complement(11740..12405)
                     /gene="tssL"
                     /locus_tag="ACHOWS_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFQ0049295.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            complement(12402..13739)
                     /gene="tssK"
                     /locus_tag="ACHOWS_003782"
     CDS             complement(12402..13739)
                     /gene="tssK"
                     /locus_tag="ACHOWS_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFQ0049296.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     SNVLFDRDALTLGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLTLNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLQEEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            complement(13759..15297)
                     /gene="tssC"
                     /locus_tag="ACHOWS_003783"
     CDS             complement(13759..15297)
                     /gene="tssC"
                     /locus_tag="ACHOWS_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFQ0049297.1"
                     /translation="MSVQQEYATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVKIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDVNLSLVSQMP
                     KAKA"
     gene            complement(15356..15856)
                     /gene="tssB"
                     /locus_tag="ACHOWS_003784"
     CDS             complement(15356..15856)
                     /gene="tssB"
                     /locus_tag="ACHOWS_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFQ0049298.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
BASE COUNT         3391 a         4530 c         4213 g         3978 t
ORIGIN      
        1 tttcgtcttc ttttttctct tcactaagta atgtctttgt acgaaaaata cagtgctttt
       61 tatgcttgtc atagatcagc ggagaaggat tttctctttc cccattactt aatattacat
      121 ttttgctcat ttttttctct ttaataatta agggtggtat ttttaaacac caagtaaaat
      181 ctcaatttca atttcttctt ctttatcact aaaaaatgtt tcggtgcatc cctcatcatc
      241 agtgacacct tccttttttg ttccatcaat aaaattggct atatacttaa cattacgata
      301 tgggatattt tcgtcatcaa aaaatttaaa acagcgagaa tagtctccac cctcaatcac
      361 cggaaacgcc atcagctccg gcgtcagctt cgccttcggc ttacgcccat aatatcccgc
      421 cttaacgtta tattctcccg gcgttcccgc ttctatccca caggcatcca gccggatgta
      481 gctgccaccg ccgttaaggg tgatcttttt cttcgccgtg attttaattt catcttcggt
      541 actggtaatg atgatttcct tcttcgccaa cagttccatc aagtcgttct gcgcctgcac
      601 cgagattgcg cccttattgg caaacagctt gatgcccgct ttacgcacaa acacgctcag
      661 cgcctgtccc acgccgataa acatattttt caccacgccg acatcggcgt tgttgcctgc
      721 gttggctatc acattctttg ccgccgccag ttgcaggtgc tcgccgctgg cgatcgccac
      781 gcctttgggt gcgcttagca ggatcaccgc ctgcgtcaga tcttttacac tctgctgcaa
      841 cagcgctatc tgtgatgaca gatctgccgg atcggcattc gccgtctgcg catcagtgga
      901 cagcgacttc atctgtgact gtgcgccatc cagtattgcc attgccggag ccatttcccg
      961 cacctgaccc tgagccttgt cctgcgcatc ggcgctgata aagatgcctt tcttcgcccg
     1021 cactgcgccc cacatatcgg tacgcagttc aaagccttcg ccgcgctgct gtttcccggc
     1081 atccaccaga tggccgaggt ttagctggct cttgccgccg tactcggtac tgaccttgat
     1141 atgctctttg ccgcgctcat cgtcgaggcg gattttgttg tttgctgggg tgcgcagcac
     1201 gttacgcttg tagttctgga tggtcacatg gtcggtgtgc gccgagtcgt gctttacccc
     1261 ggcgatatac ggtcggtccg ggttgccttc ctcaaacgcg atgctgacct ccgttcccgc
     1321 cagcagcggc agatgcaggc cgtaggtgtc tcccgcatac gggcgcgact ggcgtaccca
     1381 caggctctca taacccggtt tccacgtatc gcggtcaaaa tcgaggttga cccggtagcg
     1441 cccgtctttg tcgatatggg catagatatc gttcttcacc gtgctggtga cccgcgccgg
     1501 gagcgttccg gccatgaccg ggcgggggat aaacgccggg cggtagccgt agcgttctga
     1561 gtacgggata gcggtaaacg tcagttcata gctgcggtca cgcgccgcgc tggccgtcac
     1621 gccggtaatc agtacgccct tgcggaacac cgctggcgcg tcgtcgccct gaaccctgat
     1681 ttccagcccc ggcatcagca gtgaactggt gctctggcct ttgagaatgg cctgttcatt
     1741 caggtagcgt tcgtggcgga tacgggcata aaacgcgccg ctttccgctg cctctttatc
     1801 gcctttttgc aggaagttgt cggcgtagtg ataggcttcg ccgtaggtgg tgttatcgcc
     1861 cccggtggcg tcatgctgct ctgtcatcat ctccgctgtg gcggtgcggt agttgtagtc
     1921 ccgcgtggtg acgctttttt ccaccacgct gtacgcggtg ttcagccccc atacggcttc
     1981 cgtttcacca tcaaacagcc ctgaaggatg acgaagcggc agcgtcagcc cgcgctcata
     2041 gccgctctgg tcgtcataaa actcgacaac ctcaattttc agccgggcgt cggtggcgaa
     2101 gcggaaccag atgcccactt ccgacagcag gcgtgaaata aacgtcaggt cgtcctcgcc
     2161 atactgcatc acctgttcgc gggccgggta ttcgcttttg aggttaaaca cgaagtcctg
     2221 accgcgcatc tggtgccgct cgcgcaggat tttttcgacg atttgcggca ccgtcagatt
     2281 ctggtagatg gcattctggc ggctgcgggc cagcagcgcc atacgcggct ccagccgtac
     2341 ttcgtaacgg gcttcgtcct gtgacgatga cagatgttta aagccggtaa tgacgccata
     2401 cagggtgcgc aggggggcct gcaccggcat tccctgtacc ggcggtgccg tcaggctgaa
     2461 tgcgccgtcc tgcatcagca ccaactccgg cgtgatggct ttatcggtac tggtaaactg
     2521 aatgtcgtag cggaacgggg tactcagttg ttcgcggcct tcgaaggtca gcacatccac
     2581 cggactttgc aggccgcgga ttttgagttt gtggtgtgaa tggtcaaagt ttagggcagg
     2641 aatactcatc attatttccc tgtatagata gaaatatttt cgtcattaaa aataaatcaa
     2701 caatgaaact caatgaactc tcgccattgt ggtgtcgcat tatttttcac taagaaatcg
     2761 tttctttcat caggtgatag actgtgccag aacggagacc agatactctc ccaccagaac
     2821 tcaagttctc cttgtaagct accgaaagta tcgggttcca actcaggaaa aacatcccat
     2881 ggtacaggtt cttttattcc agcagattta atagtttcaa caaaaaaaga agagttcgtc
     2941 actaaatcaa tcaaattccc tttgaatgat ttagtgaatg tgtaaattaa agagtatgtt
     3001 ttttgtatct caatagcatc tctaaagagg gggtggtgta caatgccact atcgtaaatg
     3061 accccttcat ttccgatagt atctttattt acattaaata tattagataa atgttccttt
     3121 attgcatgaa gctcatcatc cgagcaaata aatattgagt gcgcagcgta tatccctcga
     3181 tctttcagtg aaataaatat tggataagac ccatcaacta aattgagtgg gaatttatct
     3241 tttgaaaaaa aatctttaac ttcaggtttt ttaacgagca aattcttcat gtgctctatc
     3301 caattagttt tcatctatat aatccgctac ccaatctgca ccaaaataac cagctgcacc
     3361 aaaaacaatc ccgccaataa tcccggtagc cactgctcct ggcccagtag taacacccag
     3421 ggacgcgcct atcactgttc caattttagc accagctact gctcctcccc atcctccaat
     3481 ttgccttatt gcttctgcac ctataggttt tatgcttttt gtttcaaacg atttttcagt
     3541 tgcctgttct aaatcatatg cagtgaaaaa aatactaaaa acttgtacga cacgcccggc
     3601 cttaacaatg ttttgagtcg tttttagaga ctctggtgta aaaattgttt gtgctggtac
     3661 ttttctacct tcgatcaaga cctctttatc aatatcatca acatagcctg aaattttttc
     3721 aacacgctta gccaaatgag ggtattgttt cttatactcc tcaagagcag taataatctc
     3781 tgcggtcgag attaacttca acccggcatt ttttgctttg tgaacatcaa taaatatggt
     3841 tttcccctca aatctcggag agccatcagg aaatatagag cttgtagaaa tcaacgagga
     3901 tttgttgttc cccataacat gttctgctac agtagtgggt gctgaataat cttttggcat
     3961 caacccatag cgttcaggct ctgtttctga taataatcta agaggatcca gattcatctt
     4021 tgtcaggtag gggctatttg gtgaagcata tatttttctg acaagaaagc catcttttcc
     4081 cggctcatcg ataactataa aaaagaaacc tcttgcaggc attaagggtc ctaatatatc
     4141 gtgatttgaa tcaggtatat catttgttcc tactgtattc tcagatatca ccatttttcc
     4201 agcgccttaa taccaagaag tacgtttagg ttatcagctt cttccgtgaa aattctttca
     4261 cagcacccgg cagaatcagt aaacccctta tagatattcc ctatgctgtc ttctatatag
     4321 taaggtacat tagcaatgaa ttttccgtca tcatcaatta ctctaacttg ctcatcaaat
     4381 ttcgcagata ttacaggaaa cgccatcagc tccggcgtca gcttcgcctt tggtttacgc
     4441 ccgtaatatc cggccttaac gttatattct ccctgcgttc ccgcttctat cccacaggca
     4501 tccagccgga tgtagctgcc accgccgtta agggtgattt tcttcttcgc ggtgatcttg
     4561 atctcatctt ccgtactggt gatctctatc gatttctgtg ccaacagttc catcagatcg
     4621 ttctgtgcct gtaccgagat cgcccccttg ttggcaaaca gcttgatgcc cgctttacgc
     4681 acaaacacgc ttagcgcctg tcccacgccg ataaacatat ttttcaccgc gccgacatcg
     4741 gcgttgttgc ctgcgttggc tatcacattc tttgccgccg ccagttgcag gtgctcgccg
     4801 ctggcgatcg ccacgccctt gggtgcgctt agcaggatcg cagcctggtt cagatctttt
     4861 acgctctgtt gcaacaacgc aatctgcgat gacagatctg ccggatcggc attcgccgtc
     4921 tgcgcatcag tggacagaga cttcatctgt gactgtgcgc cattcagtat cgccattgca
     4981 ggagccattt cgcgcacctg cccctgcgca ttatcctgcg catcggcgct gataaagatg
     5041 cctttctttg cccgcaccac gccccacata tcggtacgca gttcaaagcc ttcgccgcgt
     5101 tgctgtttcc cggcatccac cagatgaccg aggtttagct ggctcttgcc gccgtactcg
     5161 gtactgacct tgatatgttc tttgccgcgc tcatcgtcga ggcggatttt gttgttcgcc
     5221 ggggttctga ggacgttacg cttatagttc tgaatggtca catggtcagt gtgtgccgag
     5281 tcgtgtttta cgcctgcaat ataaggtcgg tccgggttgc cttcctcaaa ggcgatactg
     5341 acttccgttc ccgccagcag cggcaggtgc aggccgtagg tgtctcccgc atacgggcgc
     5401 gactggcgta cccacaggct ctcatagccc ggtttccacg tatcgcggtc aaagtcgagg
     5461 ttgacccggt agcgcccgtc cttgtcgata tgggcgtaga tgtcgttctt caccgtactg
     5521 gtgacccgcg ccgggagcgt tccggccatt acggggcggg ggataagcgc cggacggtag
     5581 ccgtagcgct ctgagtacgg tatcgccgtg aacgtcagct catagctgcg gtcacgcgcc
     5641 gcgctggtcg tcacgccggt aatcagcacg cccttgcgga acaccgcagg cgcgtcgtcg
     5701 ccctgaactt ttatctccag ccccggcatc agcagtgaac tggtgctctg gcctttgaga
     5761 atggcctgtt cattcaggta gcgttcgtga cggatacggg cataaaacgc gccgctttcc
     5821 gctgcctctt tatcgccttg ttgcaggaag ttgtcggcat agtggtaggc ttcgccgtaa
     5881 gtggtgttat cgcccccggt ggcgtcatgc tggcctgtgg tcatttccgc cgtggcttca
     5941 cggtagttgt agtcccgcgt ggagacactt ttttccacca cgctgtacgc ggtgttcagc
     6001 ccccatacgg cttccgtttc accatcaaac agcccagaag gatggcgaag cggcagcgtc
     6061 agcccgcgct cgtagccgct ctggtcgtcg taaaattcga taacttcgat tttcaaccgg
     6121 gcgtcggtgg caaagcggaa ccagataccc acttctgaca gcaggcgtga aataaacgtc
     6181 aggtcgtcct caccgtactg catcacctgt tcacgggacg ggtattcgct tttgaggtta
     6241 aacacgaagt cctgaccgcg catctggtgc cgctcgcgca ggattttttc gacgatttgc
     6301 ggcaccgtct ggttctggta gatggcgttc tggcggctgc gggtaagcag cgccatacgg
     6361 ggttcaaggc gcacttcgta acgggcttcg tcctgtgacg atgagagatg tttaaagccg
     6421 gtaatgacgc cgtgcagggt gcgcagggca gtctgtaccg gcattccctg taccggcggt
     6481 gccgtcaggc tgaatgcgcc atcctgcatc agcaccgact ccggctcgat ggttttatcg
     6541 gtactggtaa attcaatgtc gtagcggaag ggtgcgctga gttgttcccg gcccgtaaac
     6601 gtcagcacgt ccaccgggct tttcaggccg cgaattttga gcttgtggtg tgagtggtcg
     6661 aaattctgaa tacccataaa agcctccgtt attgcatctc ttccgtagcg aactccagta
     6721 caatcccgtc ttcctcatcc cagccaagcc gtagttgggc tggtttacgg tgcgcggcct
     6781 gctgtgccag caactgctga cttagtaccg gcaggatttg ctggttcaac aggctgtcga
     6841 tattgcgtgc accggtgtcc ggcagcaggc aggcggcggt cagcgcgtca tacaggctgt
     6901 cgttgatttc cgtttccagc ccatagtgct gatgaagacg acgaaccacc tgcgccagtt
     6961 tcatttccac aatggtgcgc atcgcctcct gtcccagcgg gcggtaaatc accgtctgaa
     7021 aacgcgccat cagtgcaggc tggaagtgct cgcgcagcag gggatacagc agttcatgca
     7081 ggtctgcgtc cgtggcgtcc ggtttttcct ccagcagttg catgatgtgg tcgctgccca
     7141 gattagccgt catcagaatc acggtgttgc ggaagtcaat ttcccggcct tcgccgtcgc
     7201 gcataaaacc ccggtcaaat acctgataga acaggttcat tacgtcgcgg tgcgcctttt
     7261 ccacttcgtc gagcaacacc acgctgtacg ggcgtttgcg aacggcttcg gtaagtacgc
     7321 cgccctgacc gtagccaacg tagcccggag gagacccctt aagctgggag acggtatgcg
     7381 gctcctgata ttcggagagg ttgatggtga taagagattt ttcaccgcca aacagggtgt
     7441 cggccagtgt gagcgcagtt tcggttttac cggtgccgct ggggccggtc agcaggaata
     7501 ccccctgtgg gccgttctcc ggcgtgaggc cggttttagc cgcccgcaac cgctgtgcga
     7561 tggcgcagag cgccgggcgt tgcccgacaa cgcggttttc aaggctctgt tccatactga
     7621 gcaggtcgct ctgctcgtcc ttgagcaggg aagagagcgg gatgccagtc cagtccgcca
     7681 ccaccgtggc gacggcgcgg gcgtccacat ccagcgccag cagcggtgca aagccctgat
     7741 gcgtccgcag ttgctgttgc agttcagagc tgtctgttcc ttcctgacgc aacgtcataa
     7801 tctgctgcac cagtgccttt tcctgctgat actgcgcttc cagatgcccg gcttctgcct
     7861 gcagctcatc acgcaaatcg gtcagttctg ccagccgctc cgggctggtt tcgggataaa
     7921 acacgctgtc ctgctcaatc gcctcccgct cgatatccag cgccgccagt cgcgcatgaa
     7981 gctgcgtcag cggctccggc agggtgtcga ggctcatgcg gactcttgct ccggcggtat
     8041 ccagcaaatc aaccgccttg tccggcagct ggcgtccggt cagatagcgg cgcgacaggc
     8101 gcacggcggt ctgaatggcg ctgtccagca tatgcacgcc gtggtgctgt gcataacggg
     8161 ccttcagtcc ccgcagcatc agacaggcgg tgtcatcatc cggctcgtca accttaacca
     8221 tctggaagcg gcgctccagc gcggcgtcgc gctcaaaata ctgtttgtat tcgctccacg
     8281 tggtcgccgc gatggtgcgc agttcgcccc ttgccagtgc cggtttcagc aggttcgccg
     8341 catccgcgcc gccagcctgg ttacccgcgc cgataatggt atgggcttca tcaataaaca
     8401 gcagtaccgg ctccggcgat ttctgtgctg catcaatcac gtttttcagt cgctgctcaa
     8461 attcaccttt aacacccgcg ccagcctgta acagaccgag gtccagcgtg cggatatgca
     8521 ccgttttcag gctgtcgggc acgttgcctt cggcgatacg cagcgccagc ccttccacca
     8581 gtgcggtttt gcccaccccc ggttctccca ccagaatcgg gttgtttttg cggcgacggg
     8641 agaggatatc gaccatctgg cgaatttcgg tgtcgcgccc gaatactggg tcgattcgcc
     8701 cgctacgggc tttttccgtc acgtcttcgg taaagcggtt aagcaccgcc agcagggcgt
     8761 catcctgttt ccccgtcagc gtttttgccg gtacgtccgt cgttgccgac tctggttgcg
     8821 cggatgtcag cggttcggtg acctgtggct gacgtgccgg acattcatcc gactgcgcat
     8881 ccagcagggg atgcagccgc tcaagctgca cgcggctcag actcagcaat acccacaggt
     8941 cattacaggc gggcagcatc ggtttttctg tcagcgccat cagcaggtgc tggctgcgga
     9001 tttgtggatc gtcaccttcc agtgacgcta tcagccacgc ctgtctgata agcgctgcca
     9061 gcggttcaga aagttgagga cgttcgcgga ccgagcgggg taaggtgtcc agatgcgcca
     9121 gcagagactg ccagagcgtg tcgatatccc attcatagcg acgggcaatc accgtgatat
     9181 cgccttctcc ctgctccagc agtttcagca gccagtgttc aatggttatc tccgcatggg
     9241 cgcgggtctg acacagtgag gcggcagcgt ccaacgcccg tgcacagtat gggttaaggc
     9301 gacgcagcag cgcggcgggg tgatttcctg tcatagaagg gtttccttat cgtgttctgt
     9361 attcctgcga tccgcgttca ggcacgctac cgcccatagc cgttacctca cacaggccca
     9421 tcaggtttca ccagattgtg ggtatgcttt gctcagtacc ggtgcctgcg gcactggaca
     9481 aagcacacaa caaaaaggca gacagtttcc tgtctgccgc agttatcagg cggaaggacg
     9541 ctcattccac gagtcggaat gaatgatgtt gccgtctttg taagtccagg tgattttctc
     9601 gtagcggaac tcaatacgtt ccaggtggtt atgcttctct ctggaaggat ccttgatgtc
     9661 gtgcatcagc ggagcgactc tgaccagctt gacgttatca agcgtgatgt tgaagtactc
     9721 cacttcctga ccggcatcgt tgatgcggta aaacttaaat tctgccgttt tcagggtctg
     9781 accggtgggc acagctttgt agagatacgg actggacgca tcgatttctt tggtaaacgt
     9841 aaagggcttg tgagtacggg taccggtcag tttgccggtg ttgttgtcgg tcgggatgta
     9901 cacatcgtga tccaacgcca ccacttcgat gctgccttcg cgcccctgaa cgtccacgga
     9961 acctttgata tctgcgccgc cgtcatcttt cagccagaga taagcaggaa tagccatgag
    10021 aattactctc cttgttagtg tgattgaagc accgccttct tccggcggta tgttttattt
    10081 cacgtcctga caggcgtcag aacgcggaac aagactgatt tcgacgcgcc ggttgactgc
    10141 cctgcccagt ggcgtatcgt tggtcgccgc aggctggctc tcacccagtc cctgtacggc
    10201 aaaacaggtg gccgggatgt cgctggtctg cagcatccag ttacgcaccg cttcggcacg
    10261 tcgcagcgat aactgctgat tgcttttttc gtcgccggtg gcgtcggtat atccggccac
    10321 gaggatcagc cagcccggtt ttgcccggat gttcaccagt gcgtccacca gcacttttgt
    10381 cgagccgtct ttcaggcggg cctgtccgac gtcaaacagc gacatactgt caagacgcac
    10441 ggtctgaacc tgaagcgaag ccgtcacctc cattttttgt tcaggcggac gccagtcgcg
    10501 aatggtgcgt aataccggct ggcggatgcg ttcgccgggg tataacccca gaccgaggcg
    10561 cagtggttct ccttcccggt aatacccatc gagcatggtc gcatcgtctt tcagcaccga
    10621 caggtgacgg gctttggtga taaattcctc caccgggacg gcataaaacc ggtgcagatc
    10681 gtcaccgacc tgccgtaata actggcgatt tgccgtggct gacaggcaca gcgcggcgat
    10741 acccgccacg gtggtgacgc ccagcatggt cacgcaggcc cgtcgcagcg gggtaaagcc
    10801 cgactgacgc ggcaaccgcc gtaacagggc atccgggaat ggcagcgcat cgtctgtgcc
    10861 ggtgtccgcg atatccggcg tcaggccggt tctggcggtg atccacaact gccataggtt
    10921 gttatccacc gcaggcaacg aggggaccag ccccacggcc atcgccagtg gtggacattt
    10981 cgcctccggg ccgttcagtt ccgccagcac attcagatcc agccattgca tcaggctttc
    11041 ggctttcagc agataagaga gtcgctgact gcgttcctgt gcgccggatt gtgcaatcca
    11101 ctgcgcgggc atggtggtct ctgcggatgt tgctacctga acttcgccgc cggtacagat
    11161 aaaccacggt gaattcgctc tctccggcaa cggcgagccg gacagcccgc tccagagaag
    11221 ccagggtacg tttacgcccg cccggcgacg cactatcgcc atactgtgag cgaaccgtcg
    11281 cagttgtccg gtcagaaccg ccgtatcccg gtacatgccg ggcatgacgg tatacgccac
    11341 ggcaagctgc gatgcccacg ccgggcgaag ggtcagtaat cgctccacct gcgccacaag
    11401 ctgttcttca tcgggaacat gcagatacag cccttctgaa acctgccgga ccgggctgtc
    11461 agtgaacaga tgcgctgaca tatcgccaca gaccagcact accggttgtc ggtacgtttc
    11521 cggcggcagc gataacaacc cggcatcgcc ggtcatctcc gtccacgtct gcctgcgccg
    11581 gtaaatcaac aatgcggcaa ccgccagaac gataactgct gcaccggccc atctcagtac
    11641 gccagacgga ctgacaaaca cccaccacag caccagcagt aacaccattc cccacagcac
    11701 gatggcctgt ttcagcgtgt tcctcactca cggctccctt taccgcaggc tttgcggcag
    11761 caattcatcc accagcacgt tcagccagtg gtgcagtccc caccagacac cggccagtag
    11821 cagcgtcagc gttaccagcc agaaaaacgg cgaatgcagc aaaggatgct gacgggggga
    11881 taacggcacg ttcagtaccg ccgtttccgg cagcacgcca aatggcgcga ccagcccgtt
    11941 gagcgtcgaa ataagctggt cacgctccgg ggccaccgga tcctgataac gcccgcgaaa
    12001 tcccagcagc aggacacggt gaaagcaggt cagcactgcc tgcgcggggg cgggctcctg
    12061 caacacctgc ttcatccgtt cgtacagcag ctcgcctgcc tgcagagtgt tgaagaaatg
    12121 cgactggagt gggtttttca tccagatggc ctgaccgtcg tccatgccac tacggctgag
    12181 caccgtttcg tccagcaggg cgcactgcgc gtaagttatg tgctccacgt tctcccgact
    12241 gaatccggca tctttcagac gttcgcggac cagttccacc tgccgctgac agtggcgata
    12301 cagctcgata ccgtgacgca cggtggttcc ctgacgcagt tccaccaccg tcagaaacgt
    12361 gtcgcgcagc agcacgtcga tattgatatc cgtctctttt ttcatgagcg cagcaccgca
    12421 aacagttcag gtttcaggtc gcccattgtt cccggcgcat aaatcatgca gcacccggac
    12481 tccagcatcg atttagccgc gtcactgtgc atatccagcg caaaatactg gttctccagc
    12541 cgcagcggca gcgccgccgg aaccgaggtg agtggcacca gtggaacacc gttcagcgcc
    12601 acgttgataa gcagcgtgac gtcatcaggc gcaccgattt tgcagaccag cggtagctgg
    12661 tgcagtacct gatgcgcagg cagtgaggaa cgaacggaca ggtaaaaatc ggcttcttcc
    12721 tgcaggcgcg gatcgtgcag gtcggctttc cagcggttgc ccggcaggga cgccagatcc
    12781 agcgcgatca cccgcgacgg caggctggct tccagcagct cgctgagcag ggagaacagt
    12841 ggcggaaaga cggtggacgg cgattcgtgg acatagggcg gaacggcgct gacgttatgc
    12901 tcaagggaaa acgtcagcag cgcaccggca agcctcgcca gctcccgcca gaccagttcg
    12961 gggtggacag ccgggtagcg caggaaatcg ctcagcaccg gctcggcgga attcagggcg
    13021 ttaagcagcc agaacagaga gacatcggcg acggcgaagt ccgccatgcg ctggttgctt
    13081 tcccggcgca tcgccatcag gcgctggcac ttcgcccgta gctgcaacag cagcgtgtcc
    13141 aggcgctgta ccagcccgtc atgggcgttt aacgtcagta acggcggaat atagctctca
    13201 tcctgcgtcc agttgccctg aacatcccgc accagacgcg ccagcggaca ggtgatgtaa
    13261 tcctgattgt tatcatgggc aaagcgcagg gacagtgcat ggcgctcaac ggccatatct
    13321 tcactgtcac tgccgtagat atcctgcacc ttttgccatt cctgacgata gcgcagcggc
    13381 ctttcagcct gctcgccctg accgagatta ccgccgttac catgcgcaag cggcagcgcc
    13441 agcaaaacaa tcgcgctgcc gccctccagc gtgcgcaggt cacaggcagg cggcagaata
    13501 tccgccacgt ctgaatcgat cagcgtacca tcagcaaaac gcaggcgtac tgcctgcgtt
    13561 ttcagctttc cgagcgtcag ggcatcccgg tcaaagagca cgtttgagat cccccacgga
    13621 tggcgaatac aaagctgcgc aatgctgtca ttgctgaacg cttcccagcg ggcctgctgc
    13681 tgaaactgct gagaagacag gaatgtcccc tcagcccaca gtggtcgaca aattttcata
    13741 catcacacca ccgcccgatc aggcttttgc ttttggcatc tgggaaacca gagaaaggtt
    13801 gacgtccatc ccttcgatct ggaaatgcgg cacggcaaag agtctgacgc ggaaaaagcc
    13861 cggattgtcc tctatgtcct cgacgataac tttcccgtcg cgcagtggat gcgaagcctg
    13921 aagttcatcg cccggatcgg tcatttccgt caccagcgtg cggatccagg tgttcagctc
    13981 cagttccagc acgcgtctgt ctttggtggt gccgatattc tcgcgctgaa tgattttcag
    14041 gtagtgagca atgcgggaca gcaggaagat atagggcaaa cgggcgttga tacggctgtt
    14101 ggcggtggcg tcagcggtat catacagcgc cggtttctgg gcagagttcg ctgagaagaa
    14161 gcaggcgtaa tcgcgattct tataataaga gagcgggata aagccaaggt tggcgaactc
    14221 aaactcgcgg gtttccggga tcatcacttc ggacggaatt ttgacctgat tgccggtgcc
    14281 gagatcgtaa agatggatcg gcagatcggc tactgcgcca cccgcctgcg gaccacggat
    14341 ctgcacacac cagccattat tcacaaaact cttcaccata ttggcggcaa aggcaaacga
    14401 ggcgtttgtc cagaggtact tttcgtgatc cgggcctttg acctcttcca catagttaaa
    14461 gctgcgtacc ggcaccgtgt ccgggccata gggcagacgg cccagcacgc gcggcatcac
    14521 taatcccacg tagcggctgt cgtcggtatc gcggaacgac ttccatttga tatattccgc
    14581 gcggtcaaaa tagttgccga tatctttaat tgcggcgact tcttccatcg agtttttaag
    14641 gaagaacttc ggtccgacgg aaccgataaa gggcatatgt gatgcagcag agactctgga
    14701 aatattacgc agcagggcaa tatcctgcgg gctgttatcg aattcatagc tggatattaa
    14761 tgaggctaca ggttcgccac ccggcgtatc atattcctga atgtatgtat ggcgatataa
    14821 accactttga atgatttccg gagaatcatc aaagtcctgc cgcagatgtt ctttactgat
    14881 atccagcagt tcaattttga catttttgcg aaaatcggtg cgctgtacga cgaaccatgt
    14941 gccgcgccag agcgactcga ctttctggaa ttccggatgg tgcatgactg catcaagctg
    15001 tttactgatt ttctgatcca aatcgtcgat atgaaaatcc agcaggctct tgtcgagttt
    15061 aaccacgtcc tcgcctgatt tcgccagaca ggaaagcaga acgtggatcg ccgccgtcac
    15121 gcgctcatcg ggtgaagcat cggccagcgt ttcgctgttt tgccataaat cgatttcctg
    15181 aatggaagag accggggtta aattaatttt atcaaacagg gactgataaa cgccgccgga
    15241 ctcagtggtg gtgagtgttg cagtttcaga ggtggcatat tcttgttgta cagacatcag
    15301 catttccttt ttagggatcc tttcttctcc ggtttaacct gaatacgcgt tcgggttaaa
    15361 cgttctcctg cggcgcaatt ttcgccagtt catcacgcag ttctgaactg agcgtcgggt
    15421 ctttcaggat attttccagt tcacggcgaa acgttgcgtt atccagcaga ttggatttca
    15481 ggtcacgtag taaattgcgc atagcgagta atacacgaag ttgcggaata ttttttgcta
    15541 cctgttcggg ttcaaaatcc ttcaggcttt taaattcaag cgcgatattt tgttcattac
    15601 cgtttccgtt tagcgtatct tctactgtta aattaaccgc aggcgaaaac tcagccatga
    15661 cgctgttgaa gttgttttta ttgatatcaa ccttgtcccg ttcggacagc ggacgttgct
    15721 cttttccgtt actgtaatcg cctacggcaa gaagcttgag ggggagttca actttctttt
    15781 tagcattgcc tgtatgcaga tcaagcttga tatttacacg agcggcggga acttcattct
    15841 ggaaactgtc ggccatatcc ttttcctcat catccacttt ttctgatggt tattgacata
    15901 attatattca ggggattatc ggatgattta aataaaaagc aatgatgagg aaaatataca
    15961 aatgaataat gtatttttca ggggaataga tacagttgta tcattttaga tattgtttgg
    16021 cctcttgatt atttttcggg agagggtggc attatctggt gagagaattt ggcatgaaac
    16081 ttaaaataat aacagggtaa gcttcacaaa tt
//
LOCUS       DAZKJR010000116        16009 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKJR010000116 DAZKJR010000000
VERSION     DAZKJR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.117"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            210..980
                     /gene="yjjP"
                     /locus_tag="ACHOWS_003785"
     CDS             210..980
                     /gene="yjjP"
                     /locus_tag="ACHOWS_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFQ0049299.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            971..1444
                     /gene="yjjB"
                     /locus_tag="ACHOWS_003786"
     CDS             971..1444
                     /gene="yjjB"
                     /locus_tag="ACHOWS_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFQ0049300.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1552..2091
                     /gene="dnaT"
                     /locus_tag="ACHOWS_003787"
     CDS             1552..2091
                     /gene="dnaT"
                     /locus_tag="ACHOWS_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFQ0049301.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2094..2831
                     /gene="dnaC"
                     /locus_tag="ACHOWS_003788"
     CDS             2094..2831
                     /gene="dnaC"
                     /locus_tag="ACHOWS_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFQ0049302.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2880..3374
                     /gene="yjjA"
                     /locus_tag="ACHOWS_003789"
     CDS             2880..3374
                     /gene="yjjA"
                     /locus_tag="ACHOWS_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFQ0049303.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3628..5919
                     /gene="opgB"
                     /locus_tag="ACHOWS_003790"
     CDS             3628..5919
                     /gene="opgB"
                     /locus_tag="ACHOWS_003790"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFQ0049304.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6057..7079)
                     /gene="lgoD"
                     /locus_tag="ACHOWS_003791"
     CDS             complement(6057..7079)
                     /gene="lgoD"
                     /locus_tag="ACHOWS_003791"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFQ0049305.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGALVAVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7218..8132
                     /gene="lgoR"
                     /locus_tag="ACHOWS_003792"
     CDS             7218..8132
                     /gene="lgoR"
                     /locus_tag="ACHOWS_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFQ0049306.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8347..9708
                     /gene="lgoT"
                     /locus_tag="ACHOWS_003793"
     CDS             8347..9708
                     /gene="lgoT"
                     /locus_tag="ACHOWS_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0049307.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMIGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9757..11421)
                     /gene="tsr"
                     /locus_tag="ACHOWS_003794"
     CDS             complement(9757..11421)
                     /gene="tsr"
                     /locus_tag="ACHOWS_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFQ0049308.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAASEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11603..12466
                     /locus_tag="ACHOWS_003795"
     CDS             11603..12466
                     /locus_tag="ACHOWS_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0049309.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            complement(12476..13945)
                     /locus_tag="ACHOWS_003796"
     CDS             complement(12476..13945)
                     /locus_tag="ACHOWS_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFQ0049310.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEELSYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
                     LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSL
                     FAIDKMGVLPALSLALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSIL
                     PAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVA
                     IMMRDVYTMITGRMPPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMS
                     GLFICSILGRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVA
                     GVLVTLVTPASQVSREEALAIITNERENQSVVITKPEEA"
     gene            complement(14048..14437)
                     /locus_tag="ACHOWS_003797"
     CDS             complement(14048..14437)
                     /locus_tag="ACHOWS_003797"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFQ0049311.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLADVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            complement(14447..15889)
                     /locus_tag="ACHOWS_003798"
     CDS             complement(14447..15889)
                     /locus_tag="ACHOWS_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0049312.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
BASE COUNT         3953 a         4259 c         3909 g         3888 t
ORIGIN      
        1 acaataaatt cattatagtg ataatattca tgcgataatt tgtttgcgac gagagataat
       61 tcgcttttta acaccgagct tctcccgata accaggatta atcagcgcaa ttttgatctt
      121 aacctgccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      181 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      241 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      301 atgagctttc ctcacgactg ggtcgggcgt tgggaatgga cagcgtcgaa agttctatct
      361 cttcgaacgc catagtgctg acaactatta aagatgggca atgcctgaca tcgacacgta
      421 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      481 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
      541 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
      601 gtaaactcaa taacggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
      661 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccacagatc aacttttgcc
      721 tcaccgcttt cgccgccacc accatttccg gtttgctttt gcaacttccc actttcagca
      781 atacccccac catcgcgatg gccgccagcg ttctgctgct ggtgccgggc tttccgttga
      841 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
      901 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
      961 ggctacgcgg atgggtgtga tcgaatttct gttagcgttg gcgcaggata tgatcctcgc
     1021 cgccattcct gcggtcggct ttgcgatggt gttcaacgtt cccgtgcggg cgttacgctg
     1081 gtgtgcgttg cttggctcga taggtcatgg ttcccgaatg atcttaatga ccagcgggct
     1141 gaatattgag tggtcaactt ttatggcttc tatgctggtc ggcaccattg gtattcaatg
     1201 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggctgtta ttcctatgtt
     1261 ccctggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agctaggtta
     1321 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1381 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1441 ataaaattgc ctcacttaaa gcacgcggat gagagacagc ctcctctcct ccgtgtgtta
     1501 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     1561 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     1621 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     1681 gccgttacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     1741 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     1801 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ctttatccgc
     1861 ctggcggcgc tatggggcgt ggcgctaaga gagccggtga ccaccgaaga actggcctca
     1921 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     1981 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2041 aatacggtca gcgaacctga cagccaaatt ccaccaggat tcagagggta acgatgaaaa
     2101 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcgt
     2161 tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc cgctccgccg
     2221 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2281 gtccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2341 atgcgttaag caaagcgcgc cagtatgtcg aagagtttga cggcaacatc gccagcttca
     2401 tcttttctgg caagcccgga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2461 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     2521 aagatacctt caggaatagc ggtaccagcg aagaacaact gcttaacgat ctgagcaacg
     2581 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     2641 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     2701 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     2761 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacgggta
     2821 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     2881 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     2941 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3001 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3061 gcagcggaaa ccaggcctta agcgcagata acatgaacaa tgccgcaggc attctgcaat
     3121 actgcgcgaa gcaaaagctg gcttcggtaa ccgacgccga aaacatcaag aatcaggtac
     3181 tggaaaaact gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3241 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataat attggaacga
     3301 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3361 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3421 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3481 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     3541 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     3601 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     3661 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     3721 ttaacggtac tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     3781 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     3841 ggtgtcagta aatacatcct gcctggtatc ggcattgtgc tggggctgac agcggtgttc
     3901 ggtgcgctgg gctggatcct gcgccgtcgt cgtcatcatc cgcaccattt tggttacagc
     3961 ctgctggcac tcttactggc gttgggttca gtggatgcca gcccggcatt tcgtcagata
     4021 acggaactgg tgaaatccca gtcacgcgac ggcgacccgg actttgcggc ttattataaa
     4081 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4141 ctcgagcgga cctattttga taacgaggct ttcccggatc tcacgcctga actgggcgcg
     4201 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4261 attgcgggca tggtggcttc tcagtgcgga ataccgctgt ttgccccctt tgaaggcaac
     4321 gcctccgctt ctgtttccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4381 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4441 gtgttcctga agtcgcatgg cttcgaccac ttatatggct cagaagagct gaaaagcgtg
     4501 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgacgaa
     4561 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     4621 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     4681 gattttgacg gtaagccgaa tcagtcattc agcgcggtaa gttgcagcca ggagaatatc
     4741 gcgacgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     4801 tcttctgacc atttagcgat gaacaacacg gcgtggaaat accttaataa acaggatcgc
     4861 aataacctgt tttttgtcat ccgtggcgac aagccgcagc aagagacgct ggcggtgaag
     4921 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     4981 ggacttggtc gtagcagttt atccgggcag tcaatgtcgg aaatcttcct caatatcaaa
     5041 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5101 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5161 ttgccgctac tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagtgaatac
     5221 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5281 gaccgttgct acaagatggc acaactttgg gctccggaac tggcactctc caccgactgg
     5341 tgtgtctcgc aagggcagct tggcggtcag caaattgtcc aacatgttga caaaacaaca
     5401 tggaagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta caaaggcaat
     5461 gtcgatacgc tgaagattgt tgataacgat attcgctaca aagccgacag tttcatcttt
     5521 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagcg ggatttcccg tccggagtcg
     5581 tggggccgct ggtccaacgc tcagctgggc gacgaagtaa aaatcgagta caagcatccg
     5641 ctgccgaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     5701 cctattccgg tacgcgtagg caatgaagaa caaactcttg tgctgggcaa tgaagtgacc
     5761 accaccacgc tgcatttcga taacccaacc gatgccgaca cgctggtaat tgtgccgccg
     5821 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     5881 ggcatggtgg aaattaaagt ggtagaacgt gaagggtaat tcctgactga tgtgtaggtc
     5941 gcataaggtg cgacctacac gccttctccg gcttacaaca cactattaag aaacaaaaaa
     6001 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6061 aaagtaatta cacccttaat caactcacga ttgttaatca catcacgctc gtaggtttct
     6121 gccagcgtgg cgaacgggta gcgatgggtt aacatcatgt cggcagtgat tttcccttcc
     6181 gccatcagtc gaccgacttt agcgaaatct tccggcgtgg cgttgcggct acccatcatc
     6241 gtcgtttctt ttttatggaa ttctggatcg gagaactgca actcaccttt aaacagaccg
     6301 acaaatacca ccgtgccgcc gtgacgaatc agattcacgg tgttattcat cgcatgttga
     6361 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgcagctgc
     6421 gcgtcaaaat cttcggctga tgggtccagc acaggcaact ccagacgcgt tgccacatgt
     6481 tcacggcgcg ccggactggt atccgccacc gctaccagtg cgccatcggc tttagcgatt
     6541 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     6601 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     6661 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     6721 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     6781 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     6841 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     6901 aaaacgcgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     6961 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7021 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7081 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7141 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7201 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7261 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7321 cggaaatgta caacatcagc cgcactacag tgcgtcatat tctcagccac ttacgcgaat
     7381 gcggcgtctt aacgcaggtt ggaaacgact atgtgattgc gcgtaaacct gaccatgatg
     7441 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7501 ttttcacgat gataaaccag cgccagttac gcccagggga aactttttct gaactgcaac
     7561 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     7621 gttataatct tattcaaagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     7681 cctacgctga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     7741 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     7801 atcggttact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     7861 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     7921 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     7981 atatcattgc cgtcgatgag catatgacca ttctcagtgc tctgatttgc cgcagtgacc
     8041 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8101 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8161 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8221 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8281 ttaatgtcaa ccttgcgtaa tgcacaatta cgtaaacaat taaaattata aatacaggag
     8341 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8401 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8461 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     8521 agttcgctgt cggttgcaaa tttaacgatt cgtgaagaat tgggattaag tgctaccgaa
     8581 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     8641 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     8701 ctgttccagg ccatgtctgg catggtgcac agctttacgc agttcgtgtt ggtgcgtatc
     8761 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     8821 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc tactattggc
     8881 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgtgggatg
     8941 tttattacca ttggtgtgct ggggattttt ctcgccattg gctggtatat gctctatcgc
     9001 aaccgcgagc acgtagaact gactgccgtt gaacaagctt acctcaatgc tggtagcgtc
     9061 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgcaca
     9121 atgtggggaa tgatgatcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9181 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9241 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttaccgac
     9301 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9361 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9421 gttctgctga ttggcatggc actgttttgt attcactttg ccggaacatc ctgctggggc
     9481 ttgatccacg tcgcagttgc ttctcgcatg acggcgtcgg tgggcagtat ccagaacttt
     9541 gccagcttca tctgcgcctc tttcgcgccg atcattactg gttttattgt tgataccacc
     9601 cactcattcc gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg
     9661 tacatcttcc tggttcgtca gccgatcaac gacccgcgta aagattaaac tccctatgcc
     9721 cgataacatt ttgcttatcg ggcattttta tggcgattaa aacgtttccc aattctcgcc
     9781 gctatctgcc acggccattt tacgcggcgt agctggcgtc acggttttta ctacagccga
     9841 tgtttcacgc tgctgttgct gctgttgctg aatccggaac actgccactg cttcggtcag
     9901 gcgactggcc tgctcttcca gcgcggcggc ggcagcggca gattcttcca ccagcgcggc
     9961 gttttgttgg gttacccggt ccatctcagc aaccgctaag ccaacctgat cgataccacg
    10021 gctctgctca tcagaagcag aagcaatttc gcccataatg tccgtcacgc gggtcacggc
    10081 actgacaatc tccgccattg tttccccggc gctttcgacc agcgtagagc caacatccac
    10141 tttccccacc gagtcttcaa tcaggctttt aatttcacga gcagcctgag cgctacgctg
    10201 ggccagatta cgcacttctc ccgcaaccac cgcaaaaccg cgcccttgct cacccgcgcg
    10261 cgctgcttca accgccgcgt tcaaagccag aatattggtc tggaaggcaa taccgtcaat
    10321 tacgctgata atatcggcga ttttctgcga actggtggag atatcgcgca tagtctgcac
    10381 cacgttatcc accactttgc cgccgcgttg cgccgtttcg gaagcactta acgccaggtg
    10441 gctggcctga cgcgcatttt cggcgttctg cttcaccgtt gcggtcagtt gctccatgct
    10501 ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg cgcgaagaga gatcgttatt
    10561 gccggtggcg atttcgctgg caccgctata gatggcattg gccccgttgc gcacatcacc
    10621 gacggtacgc atcagctctc cctgcatatg gcgcaaactc tctgccagtt gccccatctc
    10681 attagagcca tccacctcaa tcggtttcac cagatcgccg cctgcaatat gacgaatgct
    10741 gtcaatcagg cgattcattg gcgcgaccag cgaggcttta ataccgaacc agacggcgag
    10801 gatgaccgcc agtacgacga tcatcacgcc taccagaatc cacatcgcct ggctgtagga
    10861 ggcattgtta tcgctgacgg cgatatcata gagccgatcg ttttgctcca tgtaagccac
    10921 atactgcttc tcgaaaccgt cctgatatcc ctgggtcggt tgatcaaaga actcgttgat
    10981 tttgcctgca cctaacagtt ggatcagctc cgccagcgca ttgtgataaa tatcgtaatt
    11041 acgtttgatc tctgacgctg cggcggtgct ctgacgcggg tcacgcggca acgcttcgta
    11101 atccgcccag tttttttccg cctgtttcag cgaaatactg gcactctgca tcagctcagc
    11161 aacggttgaa ccgctaccaa tattattctg atccatcatg tagcggatac ccgcgcggtt
    11221 gagggtgtta cgcgtctgca acaacgcaac ccagctgcca ttcagcgtgg attgctgctg
    11281 gcgaatggtt tgtaaaacag tgaaattttc tttgtcattc tttaaggcat taaagaacag
    11341 accgcctgat gtcagttgta aaaggccaaa aacggccaaa accagcagta agctggtcac
    11401 gatcttgata cgttttaaca tggtttctct ttccactaga cagatacact ggttgtcggc
    11461 ctggtggtgg aaaactttat gctgtggaaa agcgtcggca ctgcttttgt tgcagcaaac
    11521 ttgcgttttt tgtgagggtc acactctata gcggcagttt atggtacaaa gtatcaatct
    11581 tcttcgaacc agtgatactc ctatggctta tagcatcgat atcatctcct gcattaccga
    11641 acggtttatg gcgttaacag ccactgaaaa gcgtatcgca caatttattc ttgatgacgt
    11701 ctctgccgca gtagagttgc caattgctga gctggcacgg ttaacgcaaa ccagtcaggc
    11761 gtcggtgacg cgttttgccc gtgcactggg gtgcaaagat gtccgcgagt tgaaagtcaa
    11821 actggcgcag tcactggctg tcgggcagcg gtttattctc gatgtaccgg atcttgacgg
    11881 tgttcagggg atctacgaag cgattattag cgtgctggag acgcatcgtc gttcgctgga
    11941 tatgaatgcc atcagccagg ctgtaagctg gttaagtcag gcgcggcaaa ttcttgcgtt
    12001 aggaactggc ggcggttcca ctatctgttc acaggagatt cagtaccgtt tattccgtct
    12061 ggggctaccg gtggtcagtc agaatgatgc cctgatgatg agaatgatgt gctcggcagt
    12121 catgccgcag gatgtggtga ttgtgctttc gctgggcggt tatacaccgg aaatcatcga
    12181 aagtgcggca atcgcccgtc agtatggcgc gcgtgttatt gccattacgc cagcacaaac
    12241 accgctggcg gaacaggtgg atctggtatt gccgttactg gttcaggaaa gtgattacat
    12301 ctttaagccc accccttccc gctacgcgat gttagccatg gtcgatgtac tggcaacaga
    12361 actggcaatg gcaaataaag cgcaggcgaa agatcgcctg cggcggataa aactcgtgtt
    12421 agacagccac cgtgggggcg ctaaccgcca gccacttggc gattagcatt gtcggttatg
    12481 cttcttcagg tttggtgatc acgacactct ggttttcacg ctcattggtg ataatagcca
    12541 aagcttcttc ccggctgacc tgacttgctg gcgttaccaa cgtcaccagt acgccagcga
    12601 ccaggctgcc cagcactgac ggaatacacg ggttacccca gtaagccagc cagtcggcat
    12661 tcatcagcac cacaattgac atcaccatac cgctcactaa cgccgccagc gcgccctgcc
    12721 agttaaagcg tagccagaag cgaccgagaa tcgagcagat aaacagcccg gacatcagca
    12781 tcgaaatcat tttggtaatg tagctgatga tgtcattaga ggtcagagca aagaccagcg
    12841 ccaggccaat aacaaaggtc aacatccagc gtgagtaagt aatggccttg tcctccggcg
    12901 gcattctgcc ggtaatcatg gtgtagacgt cgcgcatcat aatcgccacc gccgcgatgg
    12961 catcggagct ggcagaagac atggtggctg aaagcccggc aatcagcacc accagcccaa
    13021 gaatggcagg taagaaactg gtcgcgaaca agaaggcgta gttgctgttt tccagatttg
    13081 ggttcatggt gaacgccgcc atgccgataa tggcaggcaa aatcgagaag aacagatata
    13141 acaccccagt aataacaaac gaacggcgta cagagggaac atctttaccc gagtaaatac
    13201 gctggcgata agacggtgtt gccagcacac ctacgccaat aaccagtgcc agcgaaagcg
    13261 caggcagcac gcccatttta tcaatcgcaa acaggctcat cgcttttggg tccatcgcct
    13321 gctcaatcgc actccagccg ccaacgtgaa caacggcaag aatagccatt aatataaagc
    13381 caaagaacag aattagggcc tgaatagtat ctgtccacac taccgccgaa taaccgccga
    13441 taataacata aatcgcaaaa gctaaggcga taattaattt tgccacagta agatcaatac
    13501 ccgtcgccca ggcaagatac atacttccac ctaatatatg cgcccccagc cagccaatag
    13561 aggcaataaa tatcatcagg ccgacaatat ttttaattaa atgactaccg ccagtataat
    13621 aagagagttc ttcactcatg gtcataaaac gtaatttacg taccggtgca aacagccacg
    13681 ccaccagcaa tatacctacg gctccaccga cgccataaag cattccggcc cagccattgc
    13741 tgtagccaaa gcccactgcc cccatactgg aacccgtgcc aaccatcgtg gcaacggttg
    13801 accccagcgt cagaaacagc ggcagcgaac gcccgcccag cagaaaatct tctccgcttt
    13861 tctgattacg agaaacaaac caaccaagcc aaatcatggc gatggcataa ataataaagc
    13921 cgactaaaaa gatatgactg ttcataattc acatcctgcc agtagagaga aattaatgta
    13981 agagttcccg gagtgctgaa tgtattcagc actcaattgc ggtctgtttt tttaattctt
    14041 aaatttatta gcgggtcgcg ttataacagg tcacatcgac ttcgacttta caatcgacaa
    14101 ccagatcagc cacacaacaa atacgtgcag gcggattctc gccaaaaaat tctctgaata
    14161 ctttattaaa tgactggaaa tagcgggcgt cggtcaggat cactttcacg tgaaccacat
    14221 ccgccaggct ataaccggct tcagtcatga tgtcgacaca gttttggatc gccagtcgtg
    14281 actgttcaac gattccgcct tctaccactt caccgttttt catgggtgtt tgcccggaga
    14341 cgtacagcca gccgcccgcc tcaaccgccc tggcaaaagg caaatgctgc ccgccagtgc
    14401 ctgttccacc ttccgtaccg taacgtttaa tgctcatatt cactccttaa tgtgaggtct
    14461 gccggcgcag gaagcgtccc gcacggcctg taatatgttt atcgggacca tagctcataa
    14521 cgccgttcac catcacagcc tcaatgccat ctgccgggcg tttgggatca gaaaagctgg
    14581 caacatcacg aatggtttgt ggatcgaaca acaccaggtc cgcaaaataa ccgactttca
    14641 ccagtccacg ctggggcagg cgaaaacggc tggcagaaag cccggtcatt ttgtggatag
    14701 ccgtggtcag tgggaataag ccttcatcac ggcaatagtg gcccagcacg cgagggaagg
    14761 cgccccataa acgtggatgc ggcatgggat cattgggcag gccgtcagag ccaatcatac
    14821 tgaccggata actcaacact cggcggacgt cgcgctcgtc catgttgtaa tagatagccc
    14881 cggcaggcat tagcagcttc gcggcctcca gcatactcat ctgccagtcg atggcgattt
    14941 gcgccagtgt tttcccggcc tgctgcggat gtgtttgtga ccaggtgatc acaatatcga
    15001 attcgtcggt gacttgtttg agatccagcg ttgaagagct ggctgagtaa gggtagacat
    15061 cgcagccaat ttcctggtgt tgacgcattt ggtcaaaaaa ggccaacgtt tcttttgtcc
    15121 gcccccagtt tttcgccccg gcgcatttat ggtgggaaac cacgacaggc actttgccat
    15181 gacgcccaat acgaaacgcc tcatccaggg catcgagaat cggttcaaat tcagaacgca
    15241 gatgggtggt ataaacgccc ttctcaccgg ctaattcttc cgccagcgcc atgacttctt
    15301 cggtagtcgc ctgaaatgcc gttgcatagg ccagcccgga acttaacccc agcgccccct
    15361 gactcaacgc cagacgtaag tcggcacgca tagcggcaat ttcatccacc gtcgccgggc
    15421 gaaacaggtc gtccatatgg ttattacgca gcgccgtatg accaatcagc gtgccaacgt
    15481 tcagtgacgg cctggctgct tccactgcct gggcatagct ttcaacggta ggataaataa
    15541 agtgctctgc ttcccccaac agattcatcg ggtcaggaac ttcacctttc attttcgccg
    15601 acgccgcact aatcccgcag ttaccgacaa tcaccgtagt gatcccctgg ctgatctttg
    15661 gcagatattc cgggatgcga ataacgttga tatcgtcatg ggtatgaacg tcgataaacc
    15721 cgggtgccag cacccgccct tccccgtcaa tgacctgctg cgccgtaacg ttaagcgcag
    15781 gggcaatatc gacaatccga tcacctgtta ttgccacgtc accgcgaaat tcagggccgc
    15841 cgctaccgtc gataacggtc acatttttga ttaaccagtc aacctgcatg ttcgtcactc
    15901 catttctgca atggtgacaa gttaaccatt tacaaggacg aaaaacagtg gaaaactacg
    15961 caaaacccag atgattttat gatactttta ttcataaata ataagataa
//
LOCUS       DAZKJR010000117        15605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKJR010000117 DAZKJR010000000
VERSION     DAZKJR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.118"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..646
                     /gene="yceJ"
                     /locus_tag="ACHOWS_003799"
     CDS             80..646
                     /gene="yceJ"
                     /locus_tag="ACHOWS_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFQ0049313.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            650..1225
                     /gene="yceI"
                     /locus_tag="ACHOWS_003800"
     CDS             650..1225
                     /gene="yceI"
                     /locus_tag="ACHOWS_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFQ0049314.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1267..2319)
                     /gene="trhO"
                     /locus_tag="ACHOWS_003801"
     CDS             complement(1267..2319)
                     /gene="trhO"
                     /locus_tag="ACHOWS_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0049315.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2544..3464
                     /gene="lpxL"
                     /locus_tag="ACHOWS_003802"
     CDS             2544..3464
                     /gene="lpxL"
                     /locus_tag="ACHOWS_003802"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFQ0049316.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKTLKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3636..4862
                     /gene="mdtG"
                     /locus_tag="ACHOWS_003803"
     CDS             3636..4862
                     /gene="mdtG"
                     /locus_tag="ACHOWS_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFQ0049317.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4945..5319
                     /gene="msyB"
                     /locus_tag="ACHOWS_003804"
     CDS             4945..5319
                     /gene="msyB"
                     /locus_tag="ACHOWS_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFQ0049318.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5320..5547)
                     /gene="yceK"
                     /locus_tag="ACHOWS_003805"
     CDS             complement(5320..5547)
                     /gene="yceK"
                     /locus_tag="ACHOWS_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFQ0049319.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5719..8262)
                     /gene="mdoH"
                     /locus_tag="ACHOWS_003806"
     CDS             complement(5719..8262)
                     /gene="mdoH"
                     /locus_tag="ACHOWS_003806"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFQ0049320.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8255..9790)
                     /gene="mdoG"
                     /locus_tag="ACHOWS_003807"
     CDS             complement(8255..9790)
                     /gene="mdoG"
                     /locus_tag="ACHOWS_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFQ0049321.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10184..11341
                     /gene="mdoC"
                     /locus_tag="ACHOWS_003808"
     CDS             10184..11341
                     /gene="mdoC"
                     /locus_tag="ACHOWS_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFQ0049322.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11349..12830)
                     /gene="clsC"
                     /locus_tag="ACHOWS_003809"
     CDS             complement(11349..12830)
                     /gene="clsC"
                     /locus_tag="ACHOWS_003809"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFQ0049323.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGIFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12772..13305)
                     /gene="ymdB"
                     /locus_tag="ACHOWS_003810"
     CDS             complement(12772..13305)
                     /gene="ymdB"
                     /locus_tag="ACHOWS_003810"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFQ0049324.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13400..13711)
                     /gene="ymdA"
                     /locus_tag="ACHOWS_003811"
     CDS             complement(13400..13711)
                     /gene="ymdA"
                     /locus_tag="ACHOWS_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFQ0049325.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13832..14164)
                     /gene="csgC"
                     /locus_tag="ACHOWS_003812"
     CDS             complement(13832..14164)
                     /gene="csgC"
                     /locus_tag="ACHOWS_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFQ0049326.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14223..14678)
                     /gene="csgA"
                     /locus_tag="ACHOWS_003813"
     CDS             complement(14223..14678)
                     /gene="csgA"
                     /locus_tag="ACHOWS_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFQ0049327.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14719..15174)
                     /gene="csgB"
                     /locus_tag="ACHOWS_003814"
     CDS             complement(14719..15174)
                     /gene="csgB"
                     /locus_tag="ACHOWS_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFQ0049328.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3888 a         3990 c         3700 g         4027 t
ORIGIN      
        1 caaattgtta tttttgtgtt gcaattcatt ctccgacacg ccattttctt tctctcgcaa
       61 agtcgttttg gatgcactta tgtcattcac aaatacccct gaacgctatg gtgttatatc
      121 tgcggccttc cattggctaa gcgcaattat tgtctacggc atgtttgctt taggtctgtg
      181 gatggtcacg ctcagttatt acgatggctg gtatcaccag gcacccgaac tgcataaaag
      241 catcggtatt ttgttaatga tggggctggt tattcgcgtt ctgtggcgcg tcatatctcc
      301 accgccgggt ccgctgcaga gttattcgcc catgactcgt cttggcgcaa aggctggaca
      361 tctcgcattg tacttattgc tgttcgccat cggcatcagc ggctatctga tctcaactgc
      421 cgatggtaaa ccgatcagcg tttttggctg gtttgacgtc cccgcgaccc tcgccgacgc
      481 tggcgcacag gctgactttg ccggtgccct gcatttttgg ctcgcgtgga gcgtcgtagt
      541 actgtccgtt atgcacggat ttatggccct gaagcatcat ttcatcgata aagacgacac
      601 tctgaagcgc atgctgggaa agtcgtcatc tgactatgga gtataaaaaa tgaaaaaaag
      661 cctgcttggt ttaaccttcg cttccctgat gttctctgcc ggttcagcgg ttgccgccga
      721 ttacaaaatt gacaaagaag gtcagcacgc ctttgttaac ttccgcatac agcaccttgg
      781 ctatagctgg ttatacggca cctttaaaga tttcgacggt acttttacct ttgatgaaaa
      841 aaatccagct gccgataaag tgaatgtgac aattaacacc accagcgtcg atactaatca
      901 cgccgaacgc gataaacatc ttcgcagtgc agatttcctc aatactgcaa aatatccaca
      961 ggcaacattc acctccacca gcgtgaagaa agacggtgac gaactggata ttaccggcga
     1021 tctgacgctg aatggcgtaa ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg
     1081 cgacgaccca tggggtggta aacgtgcagg cttcgaggcc gaaggcaaaa ttaagctcaa
     1141 ggacttcaat atcaagacag atttaggtcc agcttctcag gaagtggatc tgattatttc
     1201 agtggaaggc gtacagcaga agtaagagtg aaatgttgcc ggatggcacg catccggcaa
     1261 tgatatttat tctgttggat caggaatgcc cagtgttgta ttcagacgtc cacgagactt
     1321 attaaagatc ttattgccat tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg
     1381 taacgcgctt tcttcgcagc aaatttcact acaacaacct ttgtattttt ctgcacatac
     1441 tggacactga ataaacagca ggtggcagcc atcattttta cagttggtat ggctgtcgca
     1501 cggcgcaccg cactggtggc aatgcgcgat aatctcatca gatatacgtt cgcccatccg
     1561 ctcgtcaaaa acaaaatttt tgccaataaa acgtaccggt aaaccctgct cgcgcgcctt
     1621 gcgggcgtat tcaataattc cgccctcgat atgccagact ttattgaatc cgttatgttt
     1681 catccaggca ctggcctttt cacaacgaat gccgccggtg cagtacataa cgattttttt
     1741 atctttatgt gcctgcatca tctctactgc ttttggcagc tgctcacgga aggtatctgc
     1801 cggaatttcc agcgcgtttt caaagtgccc cacttcatac tcatagtggt tacgcatgtc
     1861 gataaacaat gcatcgggat cgtcaagcat ggcgttcact tccgccgctt gcagatactc
     1921 acccacattg ctggcatcaa agtgaggatc gtcaataccg tcggcaacga tacgatcgcg
     1981 gaccttcatg cgcagtaccc agaaggattt cccgtcatca tccaacgcga tattcaggcg
     2041 taagccctct aaagccgggt cgaaggcata aagctgcgcg cgaaatgttt caacattgct
     2101 cgcaggtacg ctgatttgcg cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac
     2161 atccagcgcg gtaaacagct gatataaagc gtcacgggtc gccttaggat cggcgatgtg
     2221 gaaatacttg taaaacgaaa tggtggttcg cggttcgctc tcagccaaca ttttggcttt
     2281 taacgcgtcg ttggaaatgc ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat
     2341 gggaaagtaa aaatccgcgg catgatatag caattatcga taattaacat ccacacattt
     2401 tacgctacat ttgcgcatta aaaattattt gttatttaca agcgcggcaa tttcgcccag
     2461 tcttcagcca caaattttgg ttgcgggcga aaaaatgcga caatacatac aattgcccgt
     2521 ataggttgaa aaacaggatt gatatgacga atctacccaa gttctccacc gcactgcttc
     2581 atccgcgtta ttggttaacc tggttgggta ttggcgtact ttggttagtc gtgcaattgc
     2641 cctacccggt tatctaccgc ctcggttgtg gattaggaaa acttgcgtta cgttttatga
     2701 aacgacgcgc aaaaattgtg catcgcaacc tggaactgtg cttcccggaa atgagcgaac
     2761 aagaacgccg taaaatggtg gtgaagaatt tcgaatccgt tggcatgggc ctgatggaaa
     2821 ccggcatggc gtggttctgg ccggaccgca gaatcgcccg ctggacggaa gtgatcggca
     2881 tggaacacat tcgtgacgtg caggcgcaaa aacgcggcat cctgttagtt ggcatccatt
     2941 ttctgacact ggagcttggt gcgcggcagt ttggtatgca ggaaccgggg attggcgttt
     3001 accgcccgaa cgataatcca ctgattgact ggctacaaac ctggggccgt ttgcgctcaa
     3061 ataaatcgat gctcgaccgc aaagatttaa aaggcatgat taaaaccctg aaaaaaggcg
     3121 aagtggtctg gtacgcaccg gatcatgatt acggcccgcg ctcaagcgtt tttgtcccgt
     3181 tgtttgccgt tgagcaggct gcgaccacga ccggaacctg gatgctggca cggatgtccg
     3241 gcgcatgtct ggtgcccttc gttccacgcc gtaagccaga tggcaaaggg tatcagttga
     3301 ttatgctgcc gccagagtgt tctccgccgc tggatgatgc cgaaactacc gccgcgtgga
     3361 tgaacaaagt ggtcgaaaaa tgcatcatga tggcaccaga gcagtatatg tggttacacc
     3421 gtcgctttaa aacacgtccg gaaggcgttc cttcacgcta ttaaatctcc caagccggat
     3481 gcttgagaat agcatccggc attaccacag caaatccccc tgatttagcg ataaaagctc
     3541 tctggattgc gccccctgga agtcgggcgc ataattagtg tgcttatctt ttcttcttat
     3601 gttcaccgcg cctggcgcac caacagcgga ttgctatgtc accctgtgaa aatgacaccc
     3661 ctataaactg gaaacgaaac ctgatcgtcg cctggctagg ctgttttctt acgggtgccg
     3721 ccttcagcct ggtaatgccc ttcttacccc tctacgttga gcagcttggc gttacgggcc
     3781 actccgcctt gaatatgtgg tccggtattg tcttcagcat tacattttta ttttcggcca
     3841 tcgcctcacc gttttggggt ggactcgccg accgtaaagg ccgaaaactc atgctattac
     3901 gctctgccct cggcatgggc atcgtgatgg tgttgatggg actggcacaa aatatctggc
     3961 agtttttgat cctgcgggcg cttcttgggt tacttggcgg atttgtcccc aacgctaatg
     4021 ctcttatcgc cacacaagta ccgcgtaata aaagcggctg ggcgctgggt acgctctcca
     4081 caggcggcgt tagtggtgcg ttgctcggcc caatggctgg cggcctgctc gccgatagct
     4141 acggcttacg tccggtattc tttattaccg ccagtgtgct catactctgc tttttcgtca
     4201 ccctgttctg catcagagaa aaattccagc cggtcagcaa aaaagagatg ctgcacatgc
     4261 gggaagtggt gacatcactt aaaaacccga aactggtact cagcctgttt gtcaccacgt
     4321 taatcatcca ggtggcgaca ggctcaattg cccccattct gacgctgtat gtccgcgaac
     4381 tggcgggtaa cgtcagtaac gtcgccttta tcagtggcat gatcgcctcg gtgccaggcg
     4441 tggcggctct gctaagcgca ccacgactcg gcaaacttgg cgatcgaatc ggacccgaaa
     4501 agatcctgat tacagcgctg atcttttctg tactgctgtt gatcccaatg tcttacgttc
     4561 agacgccatt gcaacttggg attttacgtt ttttgctcgg tgccgccgat ggtgcactac
     4621 tccccgccgt acagacactg ttggtttaca actcgagcaa ccagatcgcc gggcgtatct
     4681 tcagctataa ccaatcgttt cgtgatattg gcaacgttac cggaccattg atgggagcag
     4741 cgatttcagc gaactacggt ttcagagcgg tatttctcgt caccgctggc gtcgtgttat
     4801 tcaacgcagt ctattcatgg aacagtctac gtcgtcgtcg aataccccag gtatcgaact
     4861 gatttttcgc ctttcatact tgcaaaagcg gagaatcagc tatccttttc cctgaaacct
     4921 catcaactca aagggagaat cgtgatgacc atgtacgcaa cgcttgaaga agccattgac
     4981 gctgcacgcg aagaatttct tgcagacaac cccggcatcg acgccgaaga tgcgaatgtg
     5041 caacagttca atgcccaaaa atacgttttg caggacggcg acatcatgtg gcaagttgag
     5101 ttttttgccg acgaagggga agaaggtgaa tgtttaccta tgcttagcgg tgaagccgcg
     5161 caaagtgttt ttgatggcga ctatgatgag atagagatac gccaggagtg gcaggaagag
     5221 aatacattac atgaatggga cgagggggaa tttcagcttg agccaccgct ggataccgag
     5281 gaaggacgcg cagcagctga tgagtgggat gaacgttaat cactcatacg ggccatgatg
     5341 aatgtcgatc ggcagcagta aagtatcgaa aaccagggag aatggcagat caaggatcgt
     5401 gacgtaacgc caggcggagt cacgcacgtc ccattgcaca ccgggataat attggttgcc
     5461 atgcccctgc cccggtatag tgcgactaat gatgctgcca cagccactca gcaaggtcac
     5521 cattatgctc atcacaatta aacgcatttg atacctcttc cgtaaaaaat accggcataa
     5581 cgccggtatt tttattggct cgttgttttt gccggatgcg gcgtgaacgc tttacccgac
     5641 ctacaaaatc ttgcaaaatc aataaattgc aggaacgatg taggcctgat aagcgtagcg
     5701 catcaggcaa ctacgttttt attgcgaagc cgcatccggt ttacgcaatg ccagtggatt
     5761 gagctttatc ccttcgtaat aactcaccca tgaagaatat ctctccgggg aattccagac
     5821 gcggaaatgc agacgggcca tcgtcaccgg atcgcttagc agcaccaggc gacgatcgcg
     5881 attcagcttc tctggcgtct cgttcagcgc ctgttcaacg tggcggtcac gggcgatttc
     5941 cagcaccttg ctggcgcggt gacgcgcggt cgccattgcg gttgccagag cgttaaatga
     6001 cgggttaaac accgcgtgca taaaaccatc atcaagggag cgttgacgat tcatctcaag
     6061 gaaccgatcg gtatcaacca gcacctgcgg cggtgaatac tcttccggga tcaggaacag
     6121 tttccagcgt ttggttcgca gaccaacggt ggcacggctg gaaatcaccg aaacaaacgg
     6181 tgacaggatc aacgagaaga caatcggtgc cagccagaac agaaaacgca gatccaacca
     6241 cgccatccca acagcccaca ctaaacccaa cagcagctgt gagccgtggc gtttgaatgc
     6301 ttcgccccag gaagtggagt catcatcacg ctgcggtgaa ttccacacca cttcccagcc
     6361 aaggaacgcg ctgacgacga agactgtatg gaacaacatg cgtaccggag ccagcaacac
     6421 ggaaaacagc acttccagca gcaacgataa tgtaacgcgc cagaagccgc cgtattcttt
     6481 cgttcctttg caccagataa gcaaaatgct caatagcttc ggcaggaaca acagcaccat
     6541 ggtcgaagca aaaagtgcaa tcgccagctc aggacgccac tgcggccata ccgggaacaa
     6601 ctgccgtggt tgcaggaagt attgcggttc ggtcaacgca tgtactacct gcaatgcagt
     6661 agagagcgcg aggaacataa accacagcgg agcggagaga taagacatca cgcccgtcag
     6721 gaacaccgca cggtgaaccg ggtgcatacc cttcaccagg aacagacgga agttcatcag
     6781 gttaccgtgg caccagcggc ggtcacgttt tagctcatca agcaagttag gcggcaattc
     6841 ttcataagaa cccgggagat cgtaagcaat ccacaccccc caacctgcac ggcgcatcaa
     6901 cgccgcttct acgaagtcat gtgacaggat tgaaccggca aaagaacctt cgcccggcag
     6961 cggagccagt gcacaatgct cgataaacgg tttcacgcgg ataatcgcgt tatgccccca
     7021 gtagtgcgac tcgccaagtt gccagaagtg caaaccggct gtaaacagtg gcccatacac
     7081 gcgggtcgcg aactgctgac agcgcgcata cagcgtatcc ataccggacg ctttcggcga
     7141 cgactgaatg atcccggcgt tcgggttggc ttccatcaag cgcaccaggc cgcataaaca
     7201 atcaccggtc attaccgagt cagcatccag caccaccatg tagctgtact ggctgcccca
     7261 gcgacggcag aagtcatcga tattaccgct tttacgcttc acgcgacggc ggcggcggcg
     7321 atagaaaatc tgaccttcgc caccgacttc agcgataagc tccatccagg ctttttgctc
     7381 tgcgacgcag atatccgggt tataactgtc actcagaatg tagacatcaa agtgtttggc
     7441 attcccggtg gcttttactg attcccacgt tgcacgcaag ccagcaaaaa cacggttcac
     7501 gtcttcgtta cagataggca tgatcaacgc cgtgcgatgc tccgggttta atggttcatc
     7561 gccaactgtt gacgcggata tactgtattt atcgcgacca ataagcagtt gcaggaagcc
     7621 catcaacgcc gtccagaatc cggcggacac ccaacagaac agtaccgcaa agaggatcag
     7681 gataccggtt tgcagcatat aaggcagaag ctgcataaaa gaaacccaca catcctgacc
     7741 aaccatatcc ataggattaa tcagcgccca cccctgataa ggaagaatgg tcttcatata
     7801 ccaggtcgcg acaacagttt gcgcgagcgt caggatcaac agaatgtaac ggcggatggt
     7861 accgacggta cgccactttt gctcgctctc ctgctcttct ttggtcaaac gagccagata
     7921 gcgcggcgtc acatcgcgtc cacgcaggcg atcccagaaa cggcctaccg ggttggtacg
     7981 ccacgggtcg ggaaacatcg aggagcgttt tgcttctggc atcgccttaa gctgatcgcg
     8041 cccttcgtca tctttaatta actgtccatc agcaagtgaa tctggccagg cttgttccag
     8101 acgcgccttt accgagcctt gcggggagtc atcctcccgc gcccaggtgc ggtgttcggc
     8161 atccagcgcc tgatgaacgg cgcggatatc agtcttcggc aatgccgctt tctcgcttgc
     8221 ggcgatgggc attgcgtcaa tgtactcagt tgtcttattc attggcaggt aactggtagc
     8281 tccaggtttc actcaacgtc tgatcggcat tcaccagcgc agcacgcatt tcagtggttt
     8341 tcttggcatc tttcactttc acacgcatca ccagacgcca gcctttggta accgggttat
     8401 agcgcaccgt gctttcaact atctcaccat tatcaccaat gctggtttgc gctgtgaccg
     8461 gggtatcctc tggcagtttt ttcatctctg cgccggtaaa atcgaccaca aaggcgatag
     8521 taccgtcagg ttggcgaatc aggttcgact gcttcacatc ccccgttgaa cgacgcgttt
     8581 gttgcaccca tgcgttatct ggcgcatgca gtttgtcttc atcacggctg aaggtgatgg
     8641 tgtatttaaa gttcatctct ttacccggct ccggcagctg atccggcgtc cagtaagcga
     8701 cgatgttatc gttggtttca tcgttggttg gaatttccac cagctcaacg ctgcctttgc
     8761 cccactcccc tttcggagtc acccatgcgc ttggacggag atcgtaacga tcatcgagat
     8821 cttcaaagcg ggagaaatca cgaccgcgct gcaacagacc gaagccttgc gggttttcca
     8881 tcgagaagct gctgaccgct aaatgtttcg ggttattcaa cggacgccag atccattcgc
     8941 cattaccagc atggatagag agaccgttag agtcgtgcaa ctccggacga tagttatttg
     9001 caggcgacgg ttggttcggc ccaaacagga acatactggt taacggtgca acccccagtt
     9061 tgccgacttt atcgcgcaga tagattttcg actgcacatc cacaaccgtg tcacgccctg
     9121 gcataactac gaatttgtaa gcacctgtcg cgcgcggcga gtcaagcaat gcataaatgg
     9181 ttaaacgttt atcagtcggt tttggacgct cgatccagaa ctctttgaag cgtggaaatt
     9241 cttcacccga tggcaaggcg gtatcaattg ccaggccgcg tgcagaaagg ccataaacct
     9301 gacctgcacc aatcacgcgg aaatagctgg ccccgagcat gctgacgatt tcatcgtttt
     9361 tatctttgct gttgatcggg taaagcactt taaaaccggc aaaaccaagg tcttttaccg
     9421 tgtctttgtc atgctgaaca tcgccgaaag tgaaataatc cgggctgtat ttgattcgtt
     9481 tgactgcggt ggcagtcact tcatttattt tgaccggggt atcgaagtac ataccctgat
     9541 ggtagaactc gagtttgaat ggggtcttca gattgttcca gtacgctttg tcatgattaa
     9601 actggatctg ctgatagtcc gcgtatttca tgtcgcggaa aacggagggc aagttgcttt
     9661 taggcgcctc atagcctttc ccggctaagg attgagcttg ctttgcgaca tcatcaatac
     9721 tgaaagccca gcttgaagat gtatacaggg ttaacattac tgcagcactc aaccaacgca
     9781 ttttcatcat ttgtagttta tgtttcataa ttagtaagca cttccccctt tgtgtgctta
     9841 tatcgatccg atctatttta atggataacc agccattccg acaacagaat cccggtatcg
     9901 ttcagcacgc tggctcatgg attaagcacc tgattgcaac cgttttcact ttccgtgctt
     9961 ctcctaaaga cagaagcatc gacttcgtgt agggtttgcc agcaaattta tttatcaacc
    10021 gtcttacaga cgcttccatt ctggttagtg gtagcgatga tgctatgcat aaaaaccttg
    10081 aagcctgcgc ctggcgcggt caggtcggtg tcgctatggc aacaagccta aaggcataac
    10141 ccgacaggga taagacgaaa aatccgagat tagttaacca tatatgaatc cagtacccgc
    10201 gcaacgtgaa tatttcctcg actccatccg cgcctggctg atgttgttag ggatcccttt
    10261 tcatatttct ttaatctatt cgagccatac atggcatgtg aatagcgccg aaccgtcatt
    10321 gtggctgacc ctttttaatg acttcatcca ctcgttccgc atgcaggtat ttttcgttat
    10381 atccgggtac ttttcctaca tgcttttttt acgctatccc ttgaaaaaat ggtggaaagt
    10441 acgtgtcgaa cgtgtaggta tcccgatgtt aacagccatc cccctactga cattaccgca
    10501 atttattatg ctgcaatacg tcaaagggaa agcggaaagt tggcctgggc tatcattgta
    10561 tgacaaatat aatacgttgg cctgggaatt aatatcacac ctgtggtttt tactggtgtt
    10621 agtagtcatg acgacgctgt gcgtatggat atttaaacgc atcagaaata atttagaaaa
    10681 ttctgataaa acgaataaaa aattctcgat ggtaaaacta tcggtgattt ttttgtgcct
    10741 cggcatcggt tatgcggtaa taagaagaac gatttttatt gtgtatccgc ccattctgag
    10801 taatggcatg ttcaatttta ttgtcatgca aacgctattt tatttgccgt tctttatcct
    10861 cggcgcactg gctttcattt tccctcatct taaagccttg tttaccacgc cgtctcgtgg
    10921 ctgtaccctt gcagcagcat tggcatttgt cgcttattta ctcaaccagc gctatggcag
    10981 tggcgatgcc tggatgtacg aaaccgagtc ggtgatcacc atggtcctcg gtctgtggat
    11041 ggtgaatgtg gtcttctcct ttggccaccg tttgcttaac ttccagtcag cgcgggtgac
    11101 ttattttgtt aacgcatcgc tgtttatcta tctggttcac cacccgttaa cgctgttttt
    11161 cggcgcatac attacaccgc acatcacctc caactggctt ggttttctct gtggcctgat
    11221 attcgtagta gggattgcga taattctgta tgaaattcat ttacgcatcc cgttactgaa
    11281 gtttttgttc tctggtaaac cggttgtcaa gcgtgagaac gataaagcac cagcccgtta
    11341 agccacgctt ataataacca ttccacgggc aatatcgacg ccagtctgac cataacccgc
    11401 ttccagaaac tggtggcggg ttcttttttg agaataatct ctttctttgc atgacgatca
    11461 acccagttga tccgtcccca cctgtccaga cggagctgcc aggccgcatc atactggctc
    11521 tgaataaagc gtttatcaat taactgtgcc agcgtttcgc tctctatcac gaagcccatt
    11581 tcagtattga gcaatgttga acgcggatcg aaattgaaag agccgataaa caccgtttta
    11641 ccatcgatgc taaaggtttt agcatgcaag ctggcaccgg aattaccggt tatgccgcga
    11701 tcgtgtaacg tactactttg ttcacgcgtc ggcttgagtt catataattc cacgccatag
    11761 cggagcaatt ttttgcgcca gcgcgcgtat ccggcatgga cgacagcaac atcgttagcg
    11821 gcaagagaat tggttaggat cgcaatcttt accccttttc tcaccatccg taagagttgc
    11881 gccacacctg cgcgtgtcgg tacaaaatag gaagagataa tatcgatgcg ttcactgggt
    11941 gagcccatga tatcgaacag gcgctgcggt aacagtgaat gccgttttgc cttgccttcc
    12001 cctttcgccg gatcatcact taataaacgt gttttcgccc agataagcgg caacgttcca
    12061 tcaaccagat gatttataaa cgggctggat tccattttgc gtaaataacg atgcgtcatg
    12121 gcatcgttgt gccaggatgc tggtaactcg atgcgatccg ccatttcacc ttccgggaca
    12181 tccagcacct gctgtagggg tgaaaccgat ttgcaatacc agtagcgggc gaaatcatcg
    12241 gcaacgtcct ctacaactgg tcctattgcc atgacatcta aatccgaaaa aagtggctct
    12301 tcccctgccc caaaataggc atcaccaata tttcgccccc ctaccagcgt caccacgcca
    12361 tcgacagtga aacttttatt gtgcatacgg cgattgagac gggaaaagtc ggtgatataa
    12421 ccaagcggac gtagcaagcg aaacgagaaa ggattaaaaa gccggacttc aatgcgggga
    12481 tgactgtcaa gcaagcgtaa aatgtcgtca agtccgggcg tattgttgtc gtccagcaac
    12541 aaacggacgc gaacgccacg ctttgctgcg gctaacaggg cggaaaagag taaccgaccc
    12601 gacatatcgt cctgccagat gtaatactga acatccagcg tatgctctgc catttcggcc
    12661 agacgatacc gggcggcaaa cgcatccaga cttttctcca gaggaaaaat gccacactga
    12721 ccgggatgtt gcgaacacag tggcagcacc gcactcgcca gccggggcaa atcattcatc
    12781 tccttgttga gtaaggagtc tttcgtagag gtgggcgttt tcttcatcat aacagacaaa
    12841 gtatacctgt tcgggtaaag cgtgacgggt aataaattct gaaacggttt ttactgcgat
    12901 ttcagccgct gccgcacgag ggtaaccata aactccagta ctgattgcag gaaaagccac
    12961 tgacgtatag ctgtttgctg ccaccagtcg caggctattg agataggcat cctgcaaaag
    13021 ctggtcttcg ttttgttcac caccacgcca gactggcccg acggtgtgca ctacggcttt
    13081 cgcgggaaga tcgcctgcaa gcgtaataac ggcatgcccc gtagggcaat cgccctgctg
    13141 ctgcctgact tttaaacagg catccagcag ggccggaccc gctgcgcgat gaatggcccc
    13201 atcgacgccg ccgcctccca ttaatgacgg attagccgca ttcacaatca catcaacggc
    13261 cagtttggta atatcaccct gcacaacatg aatacgcgtt ttcataacgc ctccttacac
    13321 cttcttgttt cttaagtgta cagcaggctt tttggttaaa tcgcattttt ctgctcagca
    13381 acgtgagttg ttcagaaact tagttatatt cgatgttcat tacagccagc tttttctgtt
    13441 tatcaagata ctgcactttt actgaggcta tttgcgcgtt tttgacattg ccgctcatta
    13501 acgctttgct ggaataggtt ctgcccggaa cagagccatt ctgtgggcag ttcatttcta
    13561 cgggtgatga ctgggtgctg acgtcacatg ccggttccac aatttggcca taaaaatgga
    13621 tcacgccccc ttgcgcggtg cttcctgcga tggcaataaa aggaaaaaac atactgccga
    13681 gcattagaga gtctaaaaaa ggtctgaaca tttattctat ttcctcaatg attagtcatc
    13741 cttgaggatt gtgttatcca tactttcagg ttggctgatc cgcagcagac cattctctcc
    13801 agattcatct tatgctcgat atttcaacaa attaagactt ttctgaagag ggcggccatt
    13861 gttgtgataa atgaagtgac tgtccatcag aaacagtaac aactattttc acccgatcgt
    13921 ccggggaaat atttaaactc aacttcgtca aagcaatggg ttgattagca ggcaatgaga
    13981 gggtcttttc ttgcttcgtc tgactttgcc ctgaactgcc ttcgcgcagg gacaatattt
    14041 gtactctgca cagacaagat tgagtaagag tgacttcagg aataatggtg tacatatccc
    14101 cttgctgggt cgtattaaag gttatctgac tggaaagtgc cgcaaggagt aataacgcat
    14161 tcatattctt ctcccgaaaa aaaacagggc ttgcgccctg tttctttaat acagaggatg
    14221 tattagtact gatgagcggt cgcgttgtta ccaaagccaa cctgagtgac gttgacgctg
    14281 gagttagatg cagtctggtc aacagcagca ccgttaccac caccgaactg tttaacagtc
    14341 atagtagaat ctttaccatt ccactgatca agagtagcgc tgttgccgaa accacgctga
    14401 gtcagatcga ttgagctgtc atcagaacct tggccaacat ctgcgccgtt accgccgccg
    14461 tgctgggtaa tggtcagatc agagttacgg gcgtcagctt gcagagcaag agcagagtta
    14521 ccgccaccgt actggtaaat gttcagctct gaatttgggc cgctattatt accgccacca
    14581 ccgtggttac cgccgccgcc gtactgagga acaacacctg ccagagcgct accagagaat
    14641 acgattgctg caattgctgc tacttttaaa agtttcatgt aaaaccccca tcggattgat
    14701 ttaaaagtcg aatggaaatt aacgttgtgt cacgcgaata gccatttgcg actgtctctg
    14761 cactacaatt gccgtttttt gagtaccata ctgtgtaata tttgctttat taccagaacc
    14821 tttctggata atcatcgcag tattaccata agcaccttgc gaaatactgg catcgttggc
    14881 actgcccgcc tgatcaatat atgcaaggtt ataatctcct gtctggtcaa tctttgcccg
    14941 gttgctacta ccttcttgcg caacaaccgc caaaagtttt gagcctccct gccgtaactg
    15001 agcactatta ttagtcccag cttgaccaat tatggctgcc tgattaaatg aagacttact
    15061 caattcattt accgcgaagt tatattctga attagctaaa tcataacctg ctgcggctgc
    15121 aatcccaggc gcacccagta ttgttaacat cataaataac aatttgtttt tcatgttgtc
    15181 accctggacc tggtcgtaca tttaagaaat taaatcattt caacttggtt gttaacgcaa
    15241 cctgtatttt gttaacgctg cgttacgatg gaaagtatgt ctgcggaaat atttttaata
    15301 actcacccgc gcgttgtatt tttctttttt tgttcatacc aaagtattaa aactcacgta
    15361 aatacgctgt tatctacgca aaaaatattt tgttttcttt taaatctccg ttttccgcta
    15421 tcaaaaatca ccagacaatc attcttcttg cccgtcgctg attgctgcga tatgtcttgc
    15481 gcacaagccg tgacaaacct cgtctttttg cagtaaaaaa ttgtccacgg aggtgtggag
    15541 aaaaaacaag aacgttttac atgacgaaag gactacaccg aaatattttt tatatgcatt
    15601 attag
//
LOCUS       DAZKJR010000118        15484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKJR010000118 DAZKJR010000000
VERSION     DAZKJR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.119"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(136..1344)
                     /locus_tag="ACHOWS_003815"
     CDS             complement(136..1344)
                     /locus_tag="ACHOWS_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0049329.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMT
                     HHLGYEKNQSKPGTNSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTLITG
                     MDNQIFSLYAKGMTTREIAAAFKELYDADVSPALISKVTDAVMEQVVEWQNRPSDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELPGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPGAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRN
                     LKAIYQAPTEEAGQQAQEAFAASWDSRYPHISRSWQANWPNLATFFAYATGIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQKWTMPLKDWRMAMSRFII
                     DFGDRLGGHF"
     gene            complement(1393..2687)
                     /gene="insG"
                     /locus_tag="ACHOWS_003816"
                     /pseudo
     CDS             complement(1393..2687)
                     /gene="insG"
                     /locus_tag="ACHOWS_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2801..3376)
                     /locus_tag="ACHOWS_003817"
     CDS             complement(2801..3376)
                     /locus_tag="ACHOWS_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFQ0049330.1"
                     /translation="MAVSLVKGGNISLTKEAPTMNVAMVGLGWDARVTDGQAFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQIKIDLAKVSVDV
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGAEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(3444..4022)
                     /gene="terD"
                     /locus_tag="ACHOWS_003818"
     CDS             complement(3444..4022)
                     /gene="terD"
                     /locus_tag="ACHOWS_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFQ0049331.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLTANGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDTVPSDI
                     DRIIFVVTIHDAQARRQNFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(4071..5111)
                     /gene="terC"
                     /locus_tag="ACHOWS_003819"
     CDS             complement(4071..5111)
                     /gene="terC"
                     /locus_tag="ACHOWS_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFQ0049332.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPVWPKLRGHAFLLNQKEVDAELAKPENSDVTIDRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLIHLEKAVIVLLFFIAVKLGLNATDHIWHHGYSIAATTSLYVVLGVLVLGILASVI
                     FPGKPESEEKGN"
     gene            complement(5130..5585)
                     /locus_tag="ACHOWS_003820"
     CDS             complement(5130..5585)
                     /locus_tag="ACHOWS_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFQ0049333.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFEPAEFGL"
     gene            complement(5608..6765)
                     /locus_tag="ACHOWS_003821"
     CDS             complement(5608..6765)
                     /locus_tag="ACHOWS_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFQ0049334.1"
                     /translation="MNLMPGGNAPVPAQELRVRITSGVQVDASAFRLYAAGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGCVELSGRQEAALILGEFYRRNNGWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVPPLPAETKISLSKVSLTKEMPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIYVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(6765..7346)
                     /locus_tag="ACHOWS_003822"
     CDS             complement(6765..7346)
                     /locus_tag="ACHOWS_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFQ0049335.1"
                     /translation="MVSLVKNQTVSLSKESPALSQLHFGLGWDPVKKKGFLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCKAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELACYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            7666..8724
                     /locus_tag="ACHOWS_003823"
     CDS             7666..8724
                     /locus_tag="ACHOWS_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFQ0049336.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKLFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNNQWFEEHRTAIESTGATLT
                     TGATGIDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTRLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSALPMAVFNHPEHRLVSPQLYIAAASASESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNQGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAV
                     RSITDVIVYPESLSNLLN"
     gene            8734..9876
                     /locus_tag="ACHOWS_003824"
     CDS             8734..9876
                     /locus_tag="ACHOWS_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0049337.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPILFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSENALSRRSTLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGKWDIQGKQSWGRLGMLAPAADLGHEVSFRKGER
                     VLVLGTGEFVWEPFLLAERLEAAGAQAFYGATTRSPIAVGYAIKSAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTRLLKALAEVAPVVEIVTYE"
     gene            9869..10642
                     /locus_tag="ACHOWS_003825"
     CDS             9869..10642
                     /locus_tag="ACHOWS_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049338.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLTPYQEKLASMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFSTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            10644..11723
                     /locus_tag="ACHOWS_003826"
     CDS             10644..11723
                     /locus_tag="ACHOWS_003826"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFQ0049339.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVDQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGTALDIIEERHGTSGIVFVDGWTGKG
                     AITGELVRALRDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLLSR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLAPLSWNTESTRVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIIVTEMGGTLGQYRAVTIIKKVF"
     gene            11723..12679
                     /locus_tag="ACHOWS_003827"
     CDS             11723..12679
                     /locus_tag="ACHOWS_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFQ0049340.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHEKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMRSLGKNNWPLLFIRPRHAEMGKWLKEHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLSMMPTLETENVFDVIQMRELATRLLEHPCHKRI
                     IALRIGGNDLMNVVSLRRPRALTLYDSPMGYVIKMLVSVFSPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPDQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLHNEQSADGIRLLTITQHH"
     gene            12690..13785
                     /locus_tag="ACHOWS_003828"
                     /pseudo
     CDS             12690..13785
                     /locus_tag="ACHOWS_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13803..14270
                     /gene="terW"
                     /locus_tag="ACHOWS_003829"
     CDS             13803..14270
                     /gene="terW"
                     /locus_tag="ACHOWS_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFQ0049341.1"
                     /translation="MQLNTRQARIFKLANLLGSGKPVSATDIITYLDCSEPTLTRALK
                     ELRKSYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNADLKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            14639..15292
                     /locus_tag="ACHOWS_003830"
                     /pseudo
     CDS             14639..15292
                     /locus_tag="ACHOWS_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(15353..>15484)
                     /locus_tag="ACHOWS_003831"
     CDS             complement(15353..>15484)
                     /locus_tag="ACHOWS_003831"
                     /inference="COORDINATES: protein motif:HMM:NF018724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HFQ0049342.1"
                     /translation="ENFTSVGDYIGYKKEFLQLNGFGFESVDYNHPVDIDYLMNLKP"
BASE COUNT         3898 a         3992 c         3800 g         3794 t
ORIGIN      
        1 gaaacatcag tgactgatta acaataaagt gccccaggag ccaatgccag tcagttcagc
       61 aactgactgg ctctttttcg gggctgtggg gtatttccag ggagcctgtt taggaatctg
      121 tgtaaatgcc ctttctcaga agtgaccacc caggcggtca ccgaagtcga taataaagcg
      181 gctcattgcc atacgccagt ccttcaacgg catcgtccat ttctgggacg ctgactggat
      241 tgccagccat accacttttt tcaccgagtc gtctgtcggg aacactttac gctttttgat
      301 cgcatggcgg atcacgctgt ttagcgactc gatggcattc gtcgtataga tcactttgcg
      361 gatgcccgtc gcataagcga agaacgtggc aagattcggc cagttagcct gccagcttcg
      421 gcttatatga ggatagcgac tgtcccagga cgcagcgaac gcttcctgtg cctgctggcc
      481 ggcctcttcc gtgggagcct gataaatcgc tttcaggttt cgagtgacgg ctttgtagtc
      541 cttccatgac acgaagcgca ggctgttgcg caccatatgc acgatgcata actggatgcg
      601 ggccttcgga tatactgtgt tgatggcccc cgggaagcct ttcaggccat ccacacaggc
      661 gatgaggata tcgttcagac cgcggttttt cagttcagtc agcacattga gccagaactt
      721 cgccccttca ttttcggcca gccacatacc tggcagttct ttctgacctt cgatattgat
      781 gcccagtgcc aggaacaccg atttgttaat gacgcgactg tcctgccgaa ctttaaggac
      841 gatacagtca agataaacaa tggggtaaac agcatccgac ggtcggtttt gccattctac
      901 aacctgctcc atcacggcat cggtgacctt tgatatcagt gccggtgaaa catctgcgtc
      961 atacagctct ttgaacgcag cagcgatctc acgggtggtc atccctttgg catacaacga
     1021 gaagatctgg ttatccatcc cagtaataag ggtctgattt ttctttacca gttgtggttc
     1081 gaaggtaccg tcacgatcgc gcggagtacg cagttccagt ggaccgtcgc ctgtgataac
     1141 ggtctttgtg gaataaccgt tgcgggagtt agttcccggt ttggactgat ttttctcata
     1201 cccaagatgg tgtgtcatct ctgcattgag agcggcttca acgctgatct ttttcagtag
     1261 acggtcaaac tggctgaggt cttcaggggt tttgaggttt ttggccagtt cgttagccag
     1321 agcctgtaac tgtttttcgt ccataaatta accttcattt gatgcttgat tgaacatatc
     1381 aaaatcaggc aattacacaa atctatgtac aggctctttc caaggcctct cctttaccac
     1441 tctcgggaag cccctttccc ttcttgtcgg taatttcaca agttgtccca tacttgcaag
     1501 atcgcgcatc agccccggta tacgtcccgg tgaagcgccc tgcaatgtca tcaccattcc
     1561 gcatgattct gagaaactca gttgattcgg ccagtaacct ttcagatgtt ccaccatttt
     1621 aatcatctga catcttacca gattataagc cagtaagaca ccccacagct cttgctccac
     1681 aagctccggc tttttacagc gtcagcctgc tcagttgcat cgtctgtttt atctccctgt
     1741 atcccagttc aatttcccgg cgatgactgt acagatccgc catttctccc ccggggaagc
     1801 gcatggcgtc cgtcatcgac gtcagcagat ggcagacttc tcctttgcgc gtcacggtca
     1861 gcaggcgggc tgtcacctca tttcccagtc ccggccactt ttttcgtgcc tgcggactgg
     1921 ttttcagctt caccagatga tcgcctttac ccagttttct gatctcttca tattgcgctc
     1981 cctttctgag agggatcatc cagtggcggt gttctcccac caggctccag gcatttaaca
     2041 gtcccagtga gtaataacct ttatccatta acgtcagggt gttatcgccg gtttgttcta
     2101 taagttgctc agcaagctca ttttcgctgt tcttcatcgt gccgaaggct cagccgtaag
     2161 cagatggctg gtcagttcca tctggcagac tattttgacc tgcgggtaga gcgccgggtt
     2221 gccggcatgt atctggcggg ggaaggctgc atcgttctct ggtgtatccg gtgtgcgcca
     2281 gaacatacca tcgatggcca gcagggtcag gccgcatcag tgcggatgcg gcgtggcgtt
     2341 atgccagagc tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggtgctg
     2401 gcgggcctga ataacggcac tgggggcaac gaaggggcga ttgccggcag catgatgtcc
     2461 aggcgattca caatctggtg aaggggttct ttacgctcaa gcgccatgcc aacaatacac
     2521 cagaccatca tttcgagggg aagacggcgc ttgcgtagcg ttaccgtacc tgattcggca
     2581 aggcaacgag agatgagttc ggggtcgagg taatccccca gagaagccag tgggttacgc
     2641 agagaatcgt aacgggatac cagatcaagg gcctgtccag tgtgcataaa aaatccggaa
     2701 acgagtgagc gtttccggat tcttacacaa ccactggatc ggtcaactga tccttaaccg
     2761 atcggcatta cggcattgcc ggggtcagac taagcatctt ttagacgttg acgccgtgct
     2821 gggaagcaag cgctgacagg ccaccggcaa aaccctgacc gacagcttta aacttccact
     2881 cagcgccatg gcgatacagt tcaccgaaga ccattgcggt ttcagttgag gcgtcttcag
     2941 acagatcgaa acgggcaatt tccgcgccgt tatcgttgtt gtaaacgcgc atgaagctgt
     3001 tgctcaccat gccgaaattt tgtttacgcg cttctgcatc atagatggta acggcaaata
     3061 ccagtttttt gacgtcaact gagacttttg ccagatcgat tttaatttgc tcatcgtcac
     3121 catcgccttc accggtacgg ttgtcaccct ggtgctctac tgcgccatca gggctggttt
     3181 tattattgaa gaaaatgaaa tgggcatctg acagcacttt accgtcttca cctactgcga
     3241 atacggaagc gtccagatca aaagcctgac cgtcagttac acgggcatcc cagccaagac
     3301 caaccatagc aacattcatg gtcggtgctt ctttggtcag agatatgttg ccgcctttta
     3361 cgagagaaac tgccattttt agctcctgca aacagttgaa tgagggctga atcaacagcc
     3421 cggaatgaaa agttagtttt cgatcaagac gcgttaatgc cgtactgagc acatacagat
     3481 gccagccccc ccgcataacc ctgacctaca gcacggaatt tccactcacc attgtgacga
     3541 tacagttcac cgaacagcat ggcggtttca gtggacgcat cttcagtcag atcgtagcga
     3601 gcaacttcag tctggttatc gtcattaacc aggcgaataa acgccccgga aacctgacca
     3661 aagttctgac ggcgagcctg agcatcatgg atcgtcacaa cgaagatgat cctgtcgatg
     3721 tcagacggga cagtatcaag tttaattttc agcgattcat catcaccatc cccctcacca
     3781 gtacggttat cgccggtgtg cgttacagaa ccgtcggatg acgtcaggtt gttatagaag
     3841 atgaaatctg aatcgccacg cactttgccg tttgcggtca gcaggaatgc tgaagcatcc
     3901 aagtcaaagt cctgaccgtc agttgaacgt gcatcccagc caagacccac caggacgttt
     3961 ttcattgacg gagctgcttt actcagggag acgttcccgc ctttggaaag agaaacactc
     4021 ataaaataac ctcttcgatt agtaattgct ccggttaaca cttaagggga tcagttcccc
     4081 ttttcctcag attcaggttt gccggggaaa ataacgcttg cgagaatgcc cagcaccaga
     4141 acccccagta caacatacag gctggttgtc gcagcgatgc tgtaaccatg atgccagatg
     4201 tgatcggtcg cgttcaggcc cagttttacc gcaatgaaga acaacaacac gataacagcc
     4261 ttttccagat gaatcaggta ctgtttcagt gcttcaagga caaagtacag agtacgcagc
     4321 cccaggatag caaacatcat ggcactatag acgataagcg gttcacgact gacagcaatg
     4381 attgccggta ccgagtcaaa cgcaaacata acatccgaga gttcaaccac ggccacacac
     4441 aggaacagag gggtcgcata cagcgccgcc tttttaccac ggtcaatggt tacatcactg
     4501 ttttctggtt tcgccagttc agcatccact tccttctggt taagcaggaa ggcatgccca
     4561 cgtagcttcg gccagacagg gaagaaacgt ttaaccatgc ggtaagccag atgctgggag
     4621 taatcttcaa tttcatcatc atcatcaccg cttttaagca tcatgaccgc tgtccaggca
     4681 acgatgatag cgaagacgac ttcaacgtac ggccccagac tcagcaggct cgtaccgatg
     4741 gcgacaaaga ttcccctgaa gacgatcgct ccaatgatcc cccagtaaag cacacggtgc
     4801 cgataacgat ccggaacagc gaaccaggaa aagatggcca tcatgacaaa caggttgtcg
     4861 accgacagta ctttctccag cgcatacccc gtgacaaaca aactggcaac ttcagcaccg
     4921 tggtggatat agaggaaacc ggcaaatgcc atcgcaacaa caacccagaa tacggaccag
     4981 agcgctgcac tcttcaggga gattggcttg tcatcacggt gcataaacag gtcaataaag
     5041 atggctccga ctgaaagagc aataaaaaca atgaccgttt cagtcgggaa gccgatgtgt
     5101 gtggaaacca tattttaccc ttagggatgt cagaggccaa attcagccgg ctcgaagccc
     5161 aacgcgatag cgatctcgcg ggaggccagt ttctcgtcct gatcgaagtt accgtcgctt
     5221 ttcgcaacag caataccaac acgtaaggcc agttgagcgg cctcaggctg atctttcagc
     5281 gccaggatgt atttcatggt ctcgcccttg ccgatttcaa catcgaaatc gaagcttgaa
     5341 accagtttat tgaagaactc gatcacctca ttggtatcga agaccttcag ctcttctgaa
     5401 gagcgcagaa agcccatcat tttctgcttt tcttccgaac tcacgccgtc actcgatacg
     5461 gcaatacggg cacatacagc aacggtgccc tgcatgaatt ttttgttttt gaaacgacca
     5521 acctggcggg tcagttcgtc acggccagag ttaatggcac ctttaacttt gtcgaaaaag
     5581 ctcataccag tttcctttat tgctgtgtta tttagaaccg gcgctccagc gaaatcccca
     5641 gccaaatgcc cggtccattt cgtcctggcc tttgaagtac tggttgatac gctcgacttt
     5701 aatcgcaccg ttttcgttta cgagtctggc aatggcgcac aatgtgcgac ggttttcacc
     5761 ttcagtcagg cgggtctcaa tgggcggttg atccggcaca taaatggtga ccacaccatc
     5821 agtcttgtcc caactgggaa caccttcata aataaaggcg taaatcagca cttcgcggat
     5881 atgcttccat tcacgtccat tgatatgcag ccactcgccg tctgatacat cacccgtccg
     5941 gtcatcgccc ttgagctgaa cataaggttc atcgcgataa tcaccgaatg cattaccgag
     6001 cgcctgaatg accgacttat acccgtcctg aagctcaaca aaggctccca gatccaggtc
     6061 gatgcctttg gagccgaaca tgcctgcaaa gccggatttg ccgctgcccc ggtgccagtt
     6121 caggttgatc ctgatttcac cgaagttatc ccgtttggtc agactgattg caggcatctc
     6181 ttttgtcaga gatactttac tcaggctgat ttttgtctca gctggcagag ggggtacaac
     6241 cggaggagtc ggggctgcgg gagccggttc gtcggcaata tttacgccga aatgctctgc
     6301 cagcggttta agtccaccgt tgaacccctg cgcaacgaag cggaatttcc agccattatt
     6361 acgacggtaa aattcaccca gaattaaggc tgcttcctga cggccgctca gttcgacaca
     6421 gccgctcacc aggccggtag ccccttgctc cacgtcgatg gaaagattac gaagaccgga
     6481 caccgtctgc ccaccgtcac aggtcacggt gaacgcaatt ttctggacat caggtttcag
     6541 ccggttgagc gcgacagtaa aggttgagta ctgaccttca ctgaccaggc tgacagtacc
     6601 atcatcgtta cgtggctgac cataaaacac catgtccgca tccccctgca ccttcccggc
     6661 ggcatacagg cgaaacgctg aagcatccac ctggacgccc gaggttatcc ggacccgcaa
     6721 ctcttgggct ggaacggggg cattgccccc cggcatcagg ttcattagcg aataaccgca
     6781 gaaacgatat cggagtgcat atcgtcaatt gtgcgaccgc gacaggcatg accaagggcg
     6841 gtaaagtccc agttgccgtt attacggcgc agcgaagaaa taacgatccc ggtatgtgag
     6901 ccctgctcag tcagcttgta gcaagccagc tctttgccgg tttgatcgac cacacgacag
     6961 aacgcatttt cgacgtcatt gaacgactgc ccacggaagc tgttaaccgt aaacgccagg
     7021 tattcaacgt ttgcaggcag acgggagagg ttgaccttaa tcacttcatc gtcgccgtca
     7081 ccctcaccag tcaggttatc accgctatgc acaacagctt tgcaggtgga ttccagtttg
     7141 cggaaccaga tggtgtcaat tgttttaccg gtgctgtcca ttaatacaca gccagcatcg
     7201 agatcgattg agtcgttacc tccaaacaat cctccaagaa aacctttttt cttaaccgga
     7261 tcccaaccga gaccgaaatg aagctggctt aatgcaggtg attctttgct gagtgatacc
     7321 gtctgattct taactaatga aaccatgttt tattccttaa aatgctgata actgagacaa
     7381 tcaaaagtat atttagtaac tggcgaagaa aatcatatca tgatgaagtt ctctgacaag
     7441 ggaaatttgg tagctttctg taaaaacaca aagagaaatc cttttcatga tgtcctttca
     7501 tctaaatacc aattaaatat caacacatta acaggtaaca caaaaacact gatacactcc
     7561 ataacctcag gtgttattaa tcatattatg attgacatgt agcatatacc cccataaact
     7621 cacttctcga caataaattt tcttgccgtt ccggataata aaaaaatgaa caaaaaaatt
     7681 tggtttatgg aaggtttgtc ctctcagaga gatattattc agggggttaa attatttgca
     7741 caaaaaaata attttgccat taccgttttt gcctcccacc gtaacgaaag aaatgaaatc
     7801 ctttccgttg ccgattattc tttgactgaa cctgaagatc ctcaaaaacg tcttcagttt
     7861 attcaggaaa ccattcagac ctacggcatc caccatattc atactggtcg taacaaccag
     7921 tggtttgaag aacaccgaac cgccattgaa tcgaccggtg ccacccttac taccggtgca
     7981 acgggcatcg actggttaac tctggctgac gaaaaagtta cttttgctca gtttatggag
     8041 caaaaggggc tcccggtcgt accatcctgg cgggtgaata cgctggcaga attaaagaca
     8101 cgcctcgcgg ctccgccgtt cactgacagt ccggtatgcg tgaagccggt gacgggtatc
     8161 tacggcatgg gattctggcg ctttgatgac agtgctttgc ctatggccgt ctttaatcac
     8221 cccgaacatc gtctggtcag cccgcaactg tatattgctg cagcgtcggc ttctgagtcg
     8281 tttaaacccc ttgttttgat gccgtacctg cctggcccgg aattttccgt cgatatcctc
     8341 gcggataagg gcgaaatact cgcagccgtg ggacgccgta aggaaggggc tatccagtat
     8401 ctggtaaacc aaggaagcgc ctgggaactg gcgtgtgact gcgcccgggt tatgaaggcc
     8461 gacgggctgg tgaatgttca gacgcgaaac gatgtgaatg gcaacccggt gctgcttgaa
     8521 accaacatgc gtccgtcagg cggggtgggt tatacccttc acagcggggt taaccttccc
     8581 gggttgtttg ctgcttttaa gctcggtctg atgtctgaag atgcggtgat ccagagcgcc
     8641 agaaatacct tttctccggt tgcagtgaga tccatcacag atgtcatcgt ttacccagaa
     8701 tcactctcta accttctgaa ttaaggcgtg tttatgtctg ataccctcga agatatgatt
     8761 tatcgccgta ccctctcatg cggtacgatt caggtaaccc gcgaccaggg tgaagtttcg
     8821 ctcgatgacc tgtttgatat cgctgaaaga cgtaacccga aacgcgcctt tctgtttgtc
     8881 agcaaagtac tcggcaggca cattcccgtg ccaccctcag tcatgcggca ggcctacaga
     8941 cagcttgcca gccagttccc ctcaacgctg acagggccca tactgtttat cggtatggca
     9001 gaaaccgccg ttgggcttgg ggctggtgtg tttgatgaag tgcgccacca gcatcgtgaa
     9061 tctgtctatc tgacctctac ccgtcacccg gttgatggca cattgctttg cgagttcaag
     9121 gaagaacaca gccacgccac cgatcatctg atctatctgc cagacgatga agagaaaaga
     9181 cgtcgtgtta caaacgcgcg aactctggtt ttgattgatg acgaagcaac caccggtaat
     9241 acctttatta acctgctttc tgccctgcgt aataccggca agcttcaaca tattgaacag
     9301 gttatcgctg ttacgcttac ggactggagc gaaaatgccc tgtccaggcg cagcacttta
     9361 cccgtcactt cggtttctct tgtaagtggt cagtggggat ggactccatt accagatgca
     9421 cctgtcccgg acatgccgaa ggtcaacgta acttcacgtg gaaaatggga catccaggga
     9481 aaacagtcct ggggtcgact ggggatgctc gcacctgcgg ccgatctcgg tcatgaggta
     9541 tccttccgca agggggagcg tgttctggtt ctcgggacag gggaattcgt ctgggagccg
     9601 ttcctgcttg ctgaacggct cgaagctgcc ggagcacagg cattttatgg tgcgaccacc
     9661 cgctccccta tcgccgttgg ttatgccatt aagtccgcaa tttcctttac ggataactac
     9721 ggcctgggca tccccaattt tgtctataac gtcgcccatc agcaatttga ccgcattctt
     9781 gtgtgtactg agacacctgc agaaagtatt gacacgcggc ttcttaaagc gctggctgag
     9841 gttgcacccg tcgtggagat tgttacctat gaataaaccc gttgttctga gcgaccttga
     9901 cgacacgctc tttcagaccc gtcgtaaaat ggtagacgag ctggccctgg agccattccg
     9961 taccggtgcc gttgatcgta ctctgaatcc acgaagcttt atgacagaag aacagtccat
    10021 gttagtggac tggctcctag agcaggctga actcattccc gttaccgcac ggggaactga
    10081 agaaatcagc cgcgtaagga tccctttcca ctcctgggca atcaccacgc acggggccgt
    10141 cattctcacg cctgaaggca aacccgacga ggagtggaag gcccatatgc tcggccagct
    10201 aactccctac caggaaaagc tggcatcgat gcagcgtctg atcactgaaa tgatggacgc
    10261 aaaagggatc aatgcctggg caagactgaa cttcgaatac ggtgaaacgg cggtttatat
    10321 ggtgatgaaa caccgcgaca gcactcgcct tgacgaactc aatgccattg cagatgagat
    10381 agaaacggtg ttttcgaccg agggcttcta catccaccgc aacagtaaca acgtagcctg
    10441 gcttcccacc ccggttgaga aagggctggc cgtcagctgg ctgcttgaaa aacttcgggc
    10501 tgaacgcgga gttttccccg taattggtct gggcgacagc ctgagcgatc atcgttttat
    10561 gaaactgtgc agctggtttg gcatcccgcg tcagagtcag tttgcagatg ccatttcaca
    10621 gcgaattttt ggagaaaatt aaaatgcagc cacatgaaac atttaccggc tcataccagc
    10681 ccggtgacgt ggaatttctg ctaaagccgg ttgtcattga aatgacgcca gttgatcaaa
    10741 aagaagagct gattcagtcc gggaagaaac attattcgga catgctcagc caggagccag
    10801 cgccgacaca atggcatctt gatttgtttc accgggcgct ggatcggggc gcagagagac
    10861 ttgcaaaaga agtcacacag ctcgctattg ctctggccga acgcttcggt gatgagccca
    10921 tagtactggc cagtcttgtc agggctggcg taccgctcgg cgttatgcta caccaggctc
    10981 tgcgtgacat gggaaaaacc tcatggcatt acggcatcag catcatccgg gatcgtggaa
    11041 ttgacggtac agcacttgat atcattgaag agcggcatgg taccagcggc attgtctttg
    11101 ttgacggatg gacaggtaaa ggcgcaatta ccggagagct tgtacgcgca ctgagagatc
    11161 gcccgggcta tcctgagcag ccgcgactgg ttgtcctggc cgatccctgt ggctgttcct
    11221 ggcttgcagc aagtgatgat gactggctca ttccttttgg catcatgggg gctccggtgt
    11281 caggcctgct ctcccggtca gtatggtcct ctgaaggatt acatgggtgc atggtttgcg
    11341 agcatctcag tgaattcgaa tgcagccgga tgcttgtcga taccgtcgct catttccgta
    11401 agaagttaac accgtcatcc ctcgctcctc tgagctggaa tacggagtca acccgggtct
    11461 tatggcagac aagtcgcgac gttatcgcgt tcctggccga tgaatttaaa gtggacagcg
    11521 tcaaccgtat taaacccggt attgctgaag caacccgggc tgtattacgt cgggtaccgg
    11581 accatgtatt tgtgcgttcg attgacgacc cggacgttgc cttacttgtg gggcttgctc
    11641 gtgaaaaggg aataattgtt acagaaatgg ggggaaccct cggccagtat cgggctgtaa
    11701 ccattatcaa aaaggtattc taatgaaaaa tcgtctttct ccctggaatc ttggtgccac
    11761 tctatacatg cctgcaacac gggaagatat tgctgatgcc gttctgcacg agaaaatccc
    11821 gggtttgcgc tctctggtga tttgcctgga ggatgctgtc agtgaagcag acatccccgt
    11881 cgccctcaaa aatctagagc acctgctgca cgaactcagt aacagcatgc gtagcctggg
    11941 taaaaataac tggcccctgt tgttcattcg cccccggcat gccgaaatgg gcaaatggtt
    12001 aaaagaacat tatgatcttt ccgctgtcga cgggtttgtg ttaccgaaat ttaccctaag
    12061 ttcgttagct gaatggtggg atatcatggg cggaactcac ctgagcatga tgccaacgct
    12121 ggaaacagaa aacgtctttg atgtgattca gatgcgcgag ctggccaccc ggctgttgga
    12181 acatccctgc cacaagcgca ttattgcgct tcgaatcggc ggcaacgatc ttatgaacgt
    12241 cgtgtcgctt cgccgtcccc gagccctgac gctgtatgac agtccgatgg gctacgtcat
    12301 caaaatgctc gtttcagtct tcagcccgcg tgattttgcc ctgaccgcgc ccgtatgtga
    12361 gcatattgac gatcatgccg ttatggccag agagctggct cttgatatgg cacatggact
    12421 tgttggaaaa acggccattc atccagatca gatagaggtc attcaaaacg cgctgatggt
    12481 cactcagggt gagtactctg acgctctgcg gatcctgaac tctacccagg ccgtgtttaa
    12541 gtcgcagggg gcgatgtgtg aacctgccac acatcgccgc tgggcggctg gcattctgga
    12601 cagagcccgg ttttacgggt tacataacga gcaaagcgct gatggaatca gattacttac
    12661 tataacccag catcattaag gaaatcataa tggggttcag gttcagaggt acttccgttt
    12721 ctttctgtag ccggggggcc tatgccaatc tggggttacc cggtaccgag ctgagttacc
    12781 gtacccggtt tgagctggcc gcgccttccg gaggtggaaa caggacagca accgtcccgg
    12841 atctcagaca gtcgcttgag caggaagcag ttgaactcat gtcagcggtg accgcaattc
    12901 gtaacattca cgagttgacg ctggatccaa aaacaggcat cagttgggca gagctggaag
    12961 cagtatacct gcatagcaga acatcgcttt ttcaggttcc ggcaccagtg cgtcctgaaa
    13021 aaccagaata ccatgcattg tcggaaaatc ctgcagggaa caaaggcatt gtttttctgg
    13081 gtaaatggtt tgaatcggaa tcagctaaag ctgagcgtca agcagggaat cttcgccgat
    13141 ggcggcaaga actgattgat gtgaagcgtg agaatcccct tcgacagcac cggtaccagc
    13201 aacggcagac gaccagggcc gaacagtatt caaactggaa gtgtgatgct gaagaacatg
    13261 aaaaaaggct cgccacgact caggcagatg cccggcagtt gttccgggca gacgcagcgt
    13321 ttttcgaatc ataccttgcg ggtgtgcttt cagaaactga atggccgcgt gaaacgcttg
    13381 ttgcatttga agtaaagccg gagatatcaa cagtcctgcc ggatgttggt ttagctgaga
    13441 ttgaagattt ccctgataag atttacggcg tcaatgcccg gggcatggag ctgacgcaaa
    13501 aagccatacg cgaaaactat gcccgccaca gccatggctg cttgttccga ctggtcggta
    13561 tcgtgttaca tacgctacct ttcgacaagg tgatcgcatc aggatttaca caacgagtaa
    13621 gcggaacgac tatctggagg atgtttatat cctgtcctgc aaatgcactc gcagccagaa
    13681 ggcatagaac atattgatcc ggttgaagcg ttaggctatc accctgttat tcgaaagatg
    13741 agaaacacat tcatttttca gcctattgaa ccactaaccc tttaagcaaa ctaaatatta
    13801 cgatgcaatt aaataccaga caggccagaa tttttaagct tgccaactta ctgggctccg
    13861 gcaagcctgt ttccgctacc gacattataa cctatctgga ctgctcagag ccgacattaa
    13921 cacgggccct caaagagcta cgaaagtcat attctgcaga aatcaaatac agtaaggcag
    13981 gtcattccta tcatctcgtt aatccgggtc aactggataa aaagacactt cgccggatga
    14041 atgaggcgct tgcacagaat gctgatctga aaaccggtga gtctacagga aaggtcgtac
    14101 tcgataagga taaaaaaaca gccgtatcat tatcgctacg catgcgaatt ttaagaaaaa
    14161 ttgaccgtct ggctgcactg agtggatcga cccgaagtga agctgttgaa aaactggcac
    14221 tgcattccgt tgacgaactg ataaaagaat acagcgctaa aaaatcctga tgacaaatct
    14281 cagctcgggg tgaatacccc ggttttttca tatttcaact ctgtcttggc gtgacgtatt
    14341 tttgatctat tctcctcccg caatgtcagc agctccttac agcctacaaa acctagcgtc
    14401 ttgacatcct cttgtgctac gcatcaatct ttgggcgatt tacactccac tcatgcaaat
    14461 ctgtgatgta tgatgatttt cagtaagtgt aaactgactg ccgtatgtag ccattaagcc
    14521 agtattggta acgtagatgc ccaccttttc acttatttgg tggacaccac tttgtctaac
    14581 tcgtcagatt ctgaccaaac gtttcaggct gtacgcttac tctgaagggg ggagaatagt
    14641 agcccgtgat agcattgagc taactgacag ccagatcgca gcactaaagc gtaaagccag
    14701 tgatgacgag gcctgtggcg aaagcgaaac ggctcatccg ggatatctgg gttcgcagga
    14761 cacgttctat gtaggcaacc tgaaaggtgt cgggcgtatt tatcagcaga cgttcgttga
    14821 tacgtactcg aaagtggctc actgtaagct ttacgtcacc aaaacaccga ttaccgcggc
    14881 tgacttactg aatgatcgtg tactgccgtt ttatacgtct cagggcctgc cgatgctgag
    14941 aatactgacc gacagaggca cggagtactg tggcaaagta gagcagcatg attatcagct
    15001 ttatctggca ataaacgata tagaacacac gaaaacaaag gcgatgtccc cgcagaccaa
    15061 cggcatctgc gagcggttcc ataaaacaat cctgaacgaa ttttatcagg taacgttccg
    15121 taaaaaactg tacggcgatc ctgatacatt acagtcggat cttgatgaat ggctggctca
    15181 ctataataat gagcgaagcc atcagagaaa aatgtgcaat gggcggacgc caatggaaac
    15241 gttacttgat ggaaagcgca tctgggccga gaaaaattta agccagatgt aatctgacag
    15301 atacctgtat aaataaccgg taactgtcag atcaggtctg agctaataca aattaaggtt
    15361 ttaaattcat taagtaatca atatcaactg gatgattata atccactgac tcaaatccaa
    15421 aaccatttaa ttgtaaaaat tcttttttgt atccaatata atcacctaca gaagtaaagt
    15481 tttc
//
LOCUS       DAZKJR010000119        15385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKJR010000119 DAZKJR010000000
VERSION     DAZKJR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.120"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            418..2049
                     /gene="oppA"
                     /locus_tag="ACHOWS_003832"
     CDS             418..2049
                     /gene="oppA"
                     /locus_tag="ACHOWS_003832"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFQ0049343.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIITNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2135..3055
                     /gene="oppB"
                     /locus_tag="ACHOWS_003833"
     CDS             2135..3055
                     /gene="oppB"
                     /locus_tag="ACHOWS_003833"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFQ0049344.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3070..3978
                     /gene="oppC"
                     /locus_tag="ACHOWS_003834"
     CDS             3070..3978
                     /gene="oppC"
                     /locus_tag="ACHOWS_003834"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFQ0049345.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3990..5003
                     /gene="oppD"
                     /locus_tag="ACHOWS_003835"
     CDS             3990..5003
                     /gene="oppD"
                     /locus_tag="ACHOWS_003835"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFQ0049346.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNRLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5000..6004
                     /gene="oppF"
                     /locus_tag="ACHOWS_003836"
     CDS             5000..6004
                     /gene="oppF"
                     /locus_tag="ACHOWS_003836"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFQ0049347.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6057..6386)
                     /gene="yciU"
                     /locus_tag="ACHOWS_003837"
     CDS             complement(6057..6386)
                     /gene="yciU"
                     /locus_tag="ACHOWS_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFQ0049348.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6421..7881)
                     /gene="cls"
                     /locus_tag="ACHOWS_003838"
     CDS             complement(6421..7881)
                     /gene="cls"
                     /locus_tag="ACHOWS_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0049349.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8024..8197
                     /gene="yciY"
                     /locus_tag="ACHOWS_003839"
     CDS             8024..8197
                     /gene="yciY"
                     /locus_tag="ACHOWS_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFQ0049350.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8252..9505)
                     /gene="kch"
                     /locus_tag="ACHOWS_003840"
     CDS             complement(8252..9505)
                     /gene="kch"
                     /locus_tag="ACHOWS_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFQ0049351.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9806..10102)
                     /gene="yciI"
                     /locus_tag="ACHOWS_003841"
     CDS             complement(9806..10102)
                     /gene="yciI"
                     /locus_tag="ACHOWS_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFQ0049352.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10326..11045
                     /gene="tonB"
                     /locus_tag="ACHOWS_003842"
     CDS             10326..11045
                     /gene="tonB"
                     /locus_tag="ACHOWS_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFQ0049353.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11085..11483)
                     /gene="yciA"
                     /locus_tag="ACHOWS_003843"
     CDS             complement(11085..11483)
                     /gene="yciA"
                     /locus_tag="ACHOWS_003843"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFQ0049354.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11588..12127)
                     /gene="yciB"
                     /locus_tag="ACHOWS_003844"
     CDS             complement(11588..12127)
                     /gene="yciB"
                     /locus_tag="ACHOWS_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFQ0049355.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12157..12900)
                     /gene="yciC"
                     /locus_tag="ACHOWS_003845"
     CDS             complement(12157..12900)
                     /gene="yciC"
                     /locus_tag="ACHOWS_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFQ0049356.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(12928..13017)
                     /locus_tag="ACHOWS_003846"
                     /pseudo
     CDS             complement(12928..13017)
                     /locus_tag="ACHOWS_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13257..13895
                     /gene="ompW"
                     /locus_tag="ACHOWS_003847"
     CDS             13257..13895
                     /gene="ompW"
                     /locus_tag="ACHOWS_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFQ0049357.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(13954..14460)
                     /gene="yciE"
                     /locus_tag="ACHOWS_003848"
     CDS             complement(13954..14460)
                     /gene="yciE"
                     /locus_tag="ACHOWS_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0049358.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14506..15006)
                     /gene="yciF"
                     /locus_tag="ACHOWS_003849"
     CDS             complement(14506..15006)
                     /gene="yciF"
                     /locus_tag="ACHOWS_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0049359.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETYGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(15092..15271)
                     /gene="yciG"
                     /locus_tag="ACHOWS_003850"
     CDS             complement(15092..15271)
                     /gene="yciG"
                     /locus_tag="ACHOWS_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFQ0049360.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         4089 a         3606 c         3730 g         3960 t
ORIGIN      
        1 cttaaccaat ctctatttga ttgaattgaa agatgtttgt taagacatgg atgcaagcta
       61 tagattctga tgcggtcaat aaaagagaat tgcttaacaa ttttgcaaaa tgtattggcg
      121 agtaagaagc gcatttggta ctttccgggc aaccgccagg cgattcttta ttggtaatga
      181 gaataattaa caattaaaga gcgtcgcgaa agaataatgt gtctcgacag gggagacaca
      241 gtacgaatcg acataaggtg atcgtctgaa tcaccagaat aaataaagtc ggtgatagca
      301 atccgtaacg ataaagtaac ctgacagcag aaagtctccg agcctgtgca gggtcccaat
      361 ccgggattac acatgctggt taataccagt aattataatg agggagtcca aaaaacaatg
      421 accaacatca ccaagagaag tttagtagca gctggcgttc tggctgcact aatggcaggg
      481 aatgtcgcgc tggcagctga tgtacccgca ggcgtcacac tggcggaaaa acaaacgctg
      541 gtacgtaaca atggttcaga agttcagtca ttagatccgc ataaaattga aggtgttccg
      601 gagtctaata tcagccgaga cctgtttgaa ggcttactgg tcagcgatct tgacggtcat
      661 ccggcacctg gcgtcgctga atcctgggat aataaagacg cgaaagtctg gaccttccat
      721 ttgcgtaaag atgcgaaatg gtctgatggc acgccagtca cagcacaaga ctttgtgtat
      781 agctggcaac gttctgttga tccaaacacc gcttctccgt atgccagtta tctgcaatat
      841 gggcatatcg ccggtattga tgaaattctt gaagggaaaa aaccgattac cgatctcggc
      901 gtgaaagcaa ttgatgatca cacattagaa gtcaccttaa gtgaacctgt tccgtacttc
      961 tataaattac ttgttcaccc atcaacttca ccggtgccaa aagccgctat cgagaaattc
     1021 ggcgaaaaat ggacccagcc tggtaatatc atcaccaacg gtgcctatac cttaaaagat
     1081 tgggtcgtaa acgaacgaat cgttcttgaa cgcagcccaa cctactggaa caacgcgaaa
     1141 accgttatta accaggtaac ctatttgcct attgcttctg aagttaccga tgtcaaccgc
     1201 taccgtagtg gtgaaatcga catgacgtat aacaacatgc cgatcgaatt gttccagaag
     1261 ctgaaaaaag agatcccgga cgaagttcac gttgatccat acctgtgcac ttattattac
     1321 gaaattaaca accagaaacc gccattcaac gatgtacgtg tgcgtaccgc actgaaactg
     1381 ggtatggacc gcgatatcat tgttaataaa gtgaaagcgc agggcgacat gcccgcctat
     1441 ggttacactc caccgtatac tgatggcgca aaattgactc agccggaatg gtttggctgg
     1501 agccaggaaa aacgtaacga agaagcgaaa aaactgctgg ctgaagcggg ttataccgca
     1561 gacaaaccgt tgaccatcaa cctgttgtat aacacctccg atctgcataa aaagctggcg
     1621 attgctgcct cttcattgtg gaagaaaaac attggtgtaa acgtcaaact ggttaaccag
     1681 gagtggaaaa cgttcctcga cacccgtcac cagggcactt ttgatgtggc ccgtgcaggc
     1741 tggtgtgcag actacaacga accaacttcc ttcctgaaca ccatgctgtc gaacagctca
     1801 atgaataccg cgcattataa gagcccggcc tttgacagca ttatggcgga aacgctgaaa
     1861 gcgactgacg aggcgcagcg cacagctctg tacactaaag cagaacaaca gctggataag
     1921 gattcggcca ttgttcctgt ttattactac gtgaatgcgc gtctggtgaa accgtgggtt
     1981 ggtggctata ccggcaaaga tccgctggat aatacctaca cccggaatat gtacattgtg
     2041 aagcactaat ggcaatacgt ggggcaggcg tgtactgctc cacggtgtct gatttttatc
     2101 gcattacaga aggcacaggc cagaaggtag ggcaatgtta aaatttattc tacgtcgctg
     2161 tctggaagcg attccgacgc tatttattct tattactatt tcgttcttca tgatgcgcct
     2221 cgcgccggga agccctttta ccggcgaacg tactttaccg ccagaagtga tggccaatat
     2281 cgaagcgaaa tatcatctta atgatccgat catgacacag tatttcagct acctgaaaca
     2341 actggcgcat ggcgatttcg gtccatcgtt taaatataaa gattattcgg tcaacgacct
     2401 ggttgcatcc agttttcccg tttctgccaa actgggagcc gcagcatttt tccttgcggt
     2461 aatactgggt gttagtgctg gcgttattgc cgcattaaaa caaaacacca aatgggacta
     2521 taccgtgatg gggctggcaa tgaccggggt tgttatcccc agttttgtgg ttgcgccatt
     2581 attagtcatg atatttgcga tcattttgca ttggctgccg ggcggtggct ggaatggtgg
     2641 tgcgcttaaa ttcatgatac tgccaatggt ggcgttgtca ctcgcctata tcgccagtat
     2701 tgcgcgtatt acccgtggct ctatgattga agtattacac tccaacttta ttcgtactgc
     2761 ccgggcgaaa gggttaccta tgcggcggat cattttacgc cacgcattaa aacctgctct
     2821 gttacccgtg ctctcctata tgggccccgc atttgtcggc attattaccg gttctatggt
     2881 catcgaaacc atttatggtt tgccggggat tgggcaactg ttcgttaatg gcgcattgaa
     2941 ccgtgactat tccttagtgt taagcctgac tatcctggtt ggtgcgttaa ccattttgtt
     3001 taatgccatt gtcgatgtgc tatatgcggt tatcgacccg aaaatccgtt actgatactg
     3061 gagctcgcga tgatgttaag taagaaaaac agcgagacgc tggaaaattt cagtgaaaag
     3121 ctggaggtcg aagggcgcag cttgtggcag gacgcacgtc gacgttttat gcataaccgt
     3181 gcggcggttg ccagtctgat agtgctggtg ctaatcgcgt tatttgtaat cctggcaccg
     3241 atgctttcgc agtttgccta tgacgatact gactgggcga tgatgtccag cgccccggat
     3301 atggagtccg gtcactactt tggtactgac tcatccggtc gcgacctgct tgtgcgtgtt
     3361 gcgattggcg ggcgtatctc actcatggtc ggtgttgctg cggcactggt ggcagtggtc
     3421 gtggggacac tttatggttc gctttccggt tatctgggcg gtaaagtgga ttcggtaatg
     3481 atgcgtctgc tggaaatcct caactccttc ccattcatgt tcttcgtcat tttgctggtg
     3541 acctttttcg gtcaaaacat cctgctgatt ttcgtggcga ttggcatggt ttcctggctg
     3601 gatatggctc gtattgtgcg tgggcaaacc ctgagtctga agcgcaaaga gtttattgag
     3661 gcggcacaag ttggcggtgt atcgacgtcg ggcattgtta ttcgccacat tgtgccgaat
     3721 gtactcggtg tggtggtggt ctacgcatca cttctggtgc ccagcatgat cctctttgaa
     3781 tctttcctta gcttcctggg gttggggacg caagagccgt taagcagctg gggcgcattg
     3841 ctgagtgatg gcgcgaactc gatggaagtc tctccatggt tactgttgtt cccagcggga
     3901 ttcctcgtgg tgacgctatt ttgtttcaac tttataggcg atggcttgcg tgatgccctc
     3961 gacccgaaag atcgttaagg agtgcagcca tgagcgtaat tgaaactgca actgtgccgc
     4021 tcgcacaaca acaggctgac gcactgctga acgtgaaaga tttgcgtgtc acctttagta
     4081 ccccggacgg cgacgtcacg gcggtcaatg atttgaattt ttccctacgt gccggagaaa
     4141 cgctgggcat tgtaggtgag tctggttcgg gtaaatcgca aactgcattt gcgttgatgg
     4201 gcctgctggc tgccaacgga cgtattggcg gatcggcaac cttcaatggg cgtgaaatcc
     4261 tcaatttgcc agagcgtgaa ctcaatagac tgcgcgctga acaaatctca atgatttttc
     4321 aggacccaat gacttcgttg aatccctata tgcgcgtcgg tgagcagttg atggaagtgc
     4381 tgatgctgca taagaacatg agcaaagctg aggcgtttga agagtcggtg cggatgctcg
     4441 atgcggtaaa aatgccggaa gcacgtaaac gcatgaaaat gtacccgcat gaattttctg
     4501 gtggcatgcg tcagcgagtc atgattgcga tggcattgct atgtcgacct aagctgctga
     4561 ttgcggatga acccactact gcgctggacg tcaccgtaca ggcgcagatc atgacgctat
     4621 taaatgaact gaagcgggaa tttaataccg ccatcattat gatcacccac gatcttggcg
     4681 tggtggcggg gatctgcgac aaagtgctgg taatgtacgc cgggcgcacg atggaatatg
     4741 gcaacgcgcg cgatgtcttt tatcaacccg ttcatcctta ttctatcggt ttgctcaacg
     4801 cggtgccgcg tctcgatgcg gaaggtgaaa caatgttgac catccctggt aatccgccaa
     4861 acctgctgcg attaccgaaa ggttgcccgt tccagccacg ttgtccgcat gcgatggaaa
     4921 tttgtagtag cgcaccgccg ctggaagagt ttacgcctgg ccgtctgcgt gcttgcttta
     4981 aaccggtgga ggaactgtta tgaatgctgt aaccgaagga agaaaagtcc tcctcgaaat
     5041 cgccgatctg aaagtgcact ttgaaatcaa agatggcaaa cagtggttct ggcaaccgcc
     5101 gaaaacgctc aaagccgtcg atggtgtcac tcttcgcctg tatgaagggg aaacattagg
     5161 tgtggtaggg gaatcgggat gcggtaagtc cacctttgct cgcgccatca ttggtttggt
     5221 caaggcgacc gacggtcatg ttgcctggtt aggtaaagag ttgctgggca tgaagcccga
     5281 tgaatggcgt gccgttcgca gtgatattca gatgattttc caggatccat tggcatcgct
     5341 taacccgcgt atgaccatcg gcgagatcat cgctgaacca ctgcgtactt atcatccgaa
     5401 aatgtcacgc caggaagttc gcgagcgcgt gaaggcgatg atgctgaaag tcgggttatt
     5461 gcctaacctg attaaccgct atccgcatga gttctctggt gggcagtgcc agcgtatcgg
     5521 gattgctcgt gctcttattc ttgaaccgaa gctgattatc tgtgatgagc cggtgtcggc
     5581 gctggacgtg tcaattcagg cgcaggtggt caacctgctc cagcagctgc aacgcgagat
     5641 gggattgtca ttaattttta tcgctcatga cctggccgtg gtaaaacaca tttccgatcg
     5701 tgtgctggtg atgtatctcg gccatgcggt agaactgggg acctatgatg aggtctatca
     5761 caatccgcta catccttaca ccagggcatt gatgtcggca gtccccatac ctgatccgga
     5821 tctggagaag aacaaaacca tccagttact ggaaggggaa ttaccgtcgc cgatcaaccc
     5881 gccttccggt tgtgttttcc gtacccgttg cccgattgcc ggtccagagt gtgccaaaac
     5941 acgtcctgtg ctggagggga gtttcagaca cgccgtttct tgcctgaaag tcgatccgct
     6001 ttaaaagcaa taagggctga cagttgtcag cccttattca cgccaaaagc gattatttat
     6061 tcccgccaga tgatatggca cagtttgtga tctttttcgc ggcataacag aatgcgcgcg
     6121 aaaacatcgt tgatttcacc atcttcactg tccgccagac caatcaccac ttcggcaaaa
     6181 aagtccgggt tcaaatcgaa atcaacatgt tcctgccagt cttctgccgg atcgaataac
     6241 tccgcgccgc cgcgctcttc aaactgaaga ttgaacagca gaacatcagc tggatcgaga
     6301 ttgtcggcag ccagttcgag aaaaatatcg taggcctgct caagcgtttc atcttcagtc
     6361 aggcgattgt ttagatccat atccatagtc actacctgtt taacctctgt tggcgacgtt
     6421 ttacagcaac gggctgaaga agtaaaacag tcgctcggcg acacgttgcc ataatggacg
     6481 ttttagccat aaacgggcat cgagcagacg tgaacgcgaa atataatcgt cctgaacggc
     6541 ggcaaggtct gcaccaaaac ctttatcgtc gattgccagg gtaatctcga aatttagcca
     6601 cagactccgc atatcaaggt taacggtgcc aaccagactt agttcaccat cgaccagcac
     6661 gctcttggta tgcagtaacc cgccttcaaa ctgataaatt ttaaccccag cagccagcag
     6721 ttccgtaaag aatgcgcgac tggcccagcc gaccagcatc gagtcatttt ttcgcggaag
     6781 gataatactg acatccaccc cgcgctgcgc cgccgtgcaa atcgcatgaa gtaaatcatc
     6841 gctgggcaca aagtagggcg ttgtcatgat caaatattca cgcgccgaat aagcagcagt
     6901 caataatgcc tggtgaatga gatcttccgg aaagccgggg ccagaagcaa ttgtgtgaat
     6961 ggtgtgaccg ctggcctgct caaacggcat aatattgaca tctggtggtg gcggcagaat
     7021 acgttttccg gtttcaatct cccagtcgca ggaataaata atccccatcg cggtagcgat
     7081 gggaccttcc atacgcgcca tcagatcaat ccattgccct acgcccgcat cttgtttgaa
     7141 gtagcgaggg tcgaccatat tcatgctgcc ggtgtacgcg atgtaattat cgatcatgat
     7201 catcttgcga tgttggcgca ggtccatacg gcgtaaaaac acacgcatca gattgacctt
     7261 taaggcttcg acaacttcaa taccggcatt acgcattagc tcgggccacg ggctgcggaa
     7321 aaaagccaca ctcccggcgg agtcgagcat caatcggcaa tgaatgccgc gtcgcgcagc
     7381 cgccattaat gattcagcca cctggtccgc catgccgccg ggctgccaga tataaaacac
     7441 catctcaata ttatggcgag cgagctggat gtcgcggatt aacgcctgca tcacatcatc
     7501 tgactcggtc atcagttgca gctgattccc tttgacccca gcgatcccct gacgacgctc
     7561 gcaaagcttg aataatggcg cagcgacact gctattttct tcggcgaaga tatgcttaca
     7621 ggctttaagg tcgttaagcc attttgcggt ggaaggccac atcgctctgg cgcgctcagc
     7681 gcggcgtttg cctaaatgga gctcgccaac ggcaagatag gcaataattc cgactaacgg
     7741 cagaatgtaa ataatcaaca gccaggccat cgcggaggga actgcgcgtc gtttcattag
     7801 aatgcgtaaa gttacgcctg caatgagcaa ccagtatccc agaatggcca accaactcac
     7861 caacgtataa acggttgtca tagattagaa atccttttga aagcgcattg ttatgagttt
     7921 acgcatatgg attcatctgg caaataaaaa cgcgggaaaa gcactggtca gcgtcggggg
     7981 cgggggtata atgaccattc tgttattgca tagagtagtt aacatgaagc ggagtagaac
     8041 ggaagtgggg cgctggcgca tgcagcgtca ggctagccga cgtaaatcgc gttggcttga
     8101 ggggcagtcg cgccgaaata tgcgtatcca cagcatcagg aagtgcattc taaacaaaca
     8161 gcgtaactcg ttattgtttg cgatctacaa tatctaaatg tactgggcac cattgcggtg
     8221 cccaataagt ttactcttcc ctctttaaac tctatttttg cgccgattct ttactgtcag
     8281 ctttcgtttc ttgttcatcg ttatcgctga aaatgccatg accggagttc aacaacattg
     8341 aaacgagcat atcattatta atctcttcac catttaaaac tcgcgccaga atttcgctgc
     8401 caaacagttg cggcgagaga atgatatccg gatgtaccat cttaatctta ttcaggtttt
     8461 tgctatcact gacggcgaga actgttttga catcactgct catatctttt gccgagagta
     8521 caacaaacgc gttatctgca tcgttatcac tcagcgccag aatggcccgg catcgatcaa
     8581 ttccagcttt ctttaatact gaactgtcat tactgtcacc ggggataaca tcagcgttat
     8641 cgcctaagcg ttgctcaagt tgcttgatat catcttcagg caagttgctg ataaccgtta
     8701 cgttttgtcc gcgttgattc agttgcagga tcgtattgat ggcgagaatc gaatgtccgc
     8761 aaacaataaa atgatcttta cgatgcattg tatgattgtt tccttttaca agtttgttga
     8821 atcccccgcg gataagcgga ccaaaaattg aggtcataga tgtggcaaaa acggtaatgc
     8881 cggaaataat gaccgaaata gtgaacaatc gtgctgattc agaaacaggg acaatatcgc
     8941 catagccgac ggttgacatg gtttctatcg aaaaatagaa cgcggtcatc aaactttcta
     9001 ttcgcggatt aaaaccttcg cttaaataaa gcgcaccgta ggtggagtaa aacagtaacg
     9061 tcgtgaaact aataaatgcg aaaattgtcc cggctgcggc gctactgtgg gagaagtctt
     9121 tgcgcagtat cagcaaaaac accagcgtaa aaatgcaaaa tccaatacta aatttcagcc
     9181 agggataaaa atgcagggtg taaattagcg ctatcaacaa caaaatgata ctgattgccc
     9241 atgcgagctt tgcccggaac agtaagccaa tggagttcag gacaagaaag acgccgagca
     9301 taaagagtgg ggcgtttgcc agcaaggaga gatccaactc tgaaaatgct ttgatatgga
     9361 aaatatctag caaattgacc gacataccat agattgtttt aaaaataagc aatccattta
     9421 aaaagacggc cagcgcgaga atgtcgtgcc gtagggtcac ccataaatta gttgctgttt
     9481 gtttgaatgt agcccagtga ctcactgata cctctgtaac cacaattttg tttaattatc
     9541 ttagcgtgca cattttctga acatacatgc agcgcgtgaa tgtgttaaac aatagtagat
     9601 gactttatcc gcagtaatca aaggttttga gtgagcaact ctgcgtcagg aaagggcatt
     9661 tttcctgaat atgctcacat catataaaga aatacagata aagttattat ctgcttgtgg
     9721 tggtaaatgc aatgactgac ctataaggaa aggccaaaca agaacacggt tgctaaaacc
     9781 gtgcccttaa atattgaatt tctattcaga acactttctt aaatggtttc actgaaacgt
     9841 gttcatagac tcctgccgct acgtacgggt cagcatcggc ccaggcctga gctgcttcca
     9901 gcgattcaaa ttcagcaata acggttgagc cagtaaatcc cgcagcccct ggatcgttac
     9961 tgtctactgc tggcattgga ccggcggtca acaatcgacc ttcatcatgc agtaactgta
    10021 aacgtgctaa atgtgccgga cgaacggaaa ggcgtttttc gaggctatca gctttgtctt
    10081 gagcgtaaat aacatacaac acgggcacaa ctccttgttc gggaaagttg taaagtacgt
    10141 tatttgaaag ggcgaagatc tgcaacggaa agatgatgtc tttgttaaga ccatttaaaa
    10201 agtaaggtca attacgccaa aaatgacatt ttcactgatc ctgatcgtct tgccttattg
    10261 aatatgattg ctatttgcat ttaaaatcga ggcctggttt ttctactgaa atgattatga
    10321 cttcaatgac ccttgattta cctcgccgct tcccctggcc gacgttactt tcggtctgca
    10381 ttcatggtgc tgttgtggcg ggtctgctct atacctcggt acatcaggtt attgaactac
    10441 ctgcgcctgc gcagccaatt tctgtcacga tggttgcgcc tgcggatctc gaaccgccac
    10501 aagccgttca gccgccaccg gagccggtgg tagagccaga accggaacct gagccgatcc
    10561 ccgaaccgcc aaaagaagca ccggtggtca ttgaaaagcc gaagccgaaa cctaagccaa
    10621 aaccgaagcc ggtgaaaaag gtacaggagc agccaaaacg cgatgtcaaa cccgtagagt
    10681 cgcgtccggc atcaccgttt gaaaatactg cacccgcgcg ccctacatca agtacggcaa
    10741 cggctgcaac cagcaagccg gttaccagtg tggcttcagg accacgcgca ttaagccgca
    10801 atcagccgca gtatccggca cgagcacagg cattgcgcat tgaagggcag gttaaagtta
    10861 aatttgatgt cacgccggat ggtcgcgtgg ataacgtaca aatcctctca gccaagcctg
    10921 cgaacatgtt tgagcgtgag gtgaaaaatg cgatgcgcag atggcgttat gagccgggta
    10981 agccaggcag tgggattgtg gtgaacatcc tgtttaaaat taacggcacc accgaaattc
    11041 agtaagcaga aagtcaaaag cctccgaccg gaggcttttg attattactc aacaggtaag
    11101 gcgcgaggtt ttccttcagg atcaaccgcg acatacttaa ataatgcttc tgtcgcttta
    11161 tagcgttgcc cgatgggttc agacgcgact tttttcaccc acacttcaat attaatgcta
    11221 accgatgtcg tccctttctg gacacagcgt gcatagcagc acaccacatc gccgaccgca
    11281 accggtcgta agaaagtcat tccttcaacc cgcacggtca ctacgcgacc gtgggcaatt
    11341 tctttcgcca gaatagcgcc gccaatatcc atttgtgaca ttaaccaacc accaaagatg
    11401 tcaccattgg cattggtatc ggcgggcatg gctaaagtac gtaaaacaag atcgccctga
    11461 gggacgttat gtgttgtaga catggtaaaa ccgacttaaa aggaaaatca caggcgcgat
    11521 gctactatga ttttcctgag gagaacagag gagatacagc gccagccccg aaggactggc
    11581 agtctggtta ggatttatct tcctgcggca tgtggcggta gatatagata ccgcttaaca
    11641 atgtgaagat taaggtaagg gcggtcaggc caaagacttt aaagttgacc caaatatttt
    11701 gcggcagcca gaatgcgatg tagatgtttg ccagaccgca aaggataaag aaaacagccc
    11761 aggccagatt cagcttcgac cataccggtt gcggcagcgt gagttcttta cccagcatcc
    11821 gctgaattag cggctttttc atcacccatt ggctgactaa cagggcaccc gcaaacaggg
    11881 cataaatgac agtaaccttc catttaataa actcatcatt gtggaagaac agggtcaagc
    11941 caccgaagac gaccaccaga acaaaagtga tcagggccat cttctcaacc ttacgaaagc
    12001 gaacccagct atatataagc acaatcgccg tggcgacgat cagcgccgca gtagccgcat
    12061 agatgtcata aatcttgtaa aacgcgaaaa agacaaccag cggtaaaaaa tcaagaaact
    12121 gcttcatttt acgattccgt aatcaaatgc ttaaggttat tggcgaatca acatatacag
    12181 gcggaacaga tagatgagca gtacggctga aatcaggttg ctcaaggtgt tcgccagtac
    12241 ggcaccaatt tccggggtta atgcggcaaa agaagaggca aaaagcagca acagtgtctt
    12301 tgccagcaac cagctcagta ctgcgggtgc caccagacgc atattcgccc aagtcagccg
    12361 catactgcta cgcatcgagg caaaaacgcc cattttgtcc tgtaccaaca tcaccggtgc
    12421 cagggccagt aaaatggcca tgataattcc cggaacgacc accagcatga tgccaatctg
    12481 tactaaaagg gtagtcagaa aaatcagaat aaataacttt ggcaatatcg gcgcactggc
    12541 accaatagct cgcagcgcac tgactctctg acccgcagac accagctgga taattaatat
    12601 tacgcctccg gcgagaatgg cgttaccgat taatcctgaa aaagtggacg ccgctgaagc
    12661 ctgcagcaaa atttgttgct gttccggtga catattctga accaggtcga acaacccact
    12721 actgccgcta acgggcacgc cgtcattgag ctgcgcaagc tgtgcatcac tgggtgagaa
    12781 aacatgccct aacaccactg tgataaacgc acatagcaac gataccaaca gaatggtcat
    12841 aaattgatta cggaagaaat ttccggtgtc acggtatacg gactgcgccg tgatagacat
    12901 gcactctcct tgagttttat gcaggtgtta attagcgggt aattgtaccc tggttaagcc
    12961 tcgggtggca gcatcaggtc ttgtatggaa aagcatatct ttgtaaagcg gaggtaattg
    13021 ctggccttca gacgtgggca aatcgcggca agtggcgatc gagttaatat ttgcgtagcg
    13081 aaaatattta aaaattgatt taaatcacat taaccaggat tctcaatgca acttctaaat
    13141 taatccagat caataaatgg tgaattatca tatgtaatgt gatctatgta ggatcatttg
    13201 ttactccaat gtaggtatat tcgtcacgtt tttataacca taacgacgga gcggatatga
    13261 aaaagttaac agtggcggct ttggcagtaa caactcttct ctctggcagt gcctttgcgc
    13321 atgaagcagg cgaatttttt atgcgtgcag gttctgcaac cgtacgtcca acagaaggtg
    13381 ctggtggtac gttaggaagt ctgggtggat tcagcgtgac caataatacg caactgggcc
    13441 tgacgtttac ttatatggcg accgataaca ttggtgtgga attgctggca gcgacgccgt
    13501 tccgccataa aatcggcacc cgggcgaccg gcgatattgc aaccgttcat catctgccgc
    13561 caacactgat ggcgcagtgg tattttggtg atgccagcag caaattccgt ccttacgttg
    13621 gggcaggtat taactacacc accttctttg ataatggatt taacgatcat ggcaaagagg
    13681 cggggctttc cgatctcagt ctgaaagatt cctggggagc tgccgggcag gtgggggttg
    13741 attatctgat taaccgtgac tggttggtta acatgtcagt ctggtacatg gatattgata
    13801 ccactgccaa ttataaactg ggcggtgcac agcaacacga tagcgtacgc ctcgatccgt
    13861 gggtgtttat gttctcagca ggatatcgtt tttaattccg cacaaaaatg accccgtaat
    13921 tacggggtca ataaggacat ggtataaagg gtattatttc ttcgcttcta cgccatcagt
    13981 ttcagagcga attaagaatt tctcagttgt ttgcggaata tgctgaatta gccagtcggc
    14041 catgtgcttt tcatcattta aaatcgcttc gataatcgga attgaggctg tatcaccggc
    14101 attttttgct gctgctaaga gtgaggtata acaggcgatt tcaaattgct cgaagacata
    14161 tccgctaata gagcctttga ctatttcatc agaagggaat ataccaccga ttgactgccc
    14221 aagtgcagcc attttactca tggaatcttt aatgactgaa cgtgaaatgt cattacgatc
    14281 aagaatagtt tccagttgaa caatctggtt tttggtttca ctaagatgtt gttcaatacg
    14341 agcgcgtagt tcaggataat tatctatacg actggccatg gattcaagca ttgattcggc
    14401 ttgcttttcc attgcgtggg cgtcacgtaa ccagtcatga taatgttcaa tacgattcat
    14461 gaaaatgtcc tctttataaa atgactaaaa gttaaagttc atatttcagg ctttattttc
    14521 ggctttctta tttacgttat taagggccag atcagtcaat ttgatgtcgg tggccttttc
    14581 ttcttccagg gtctctttca gaagcttcgc tgctttacga taacctaatt gttcagccag
    14641 cgtcgctaat gtcccgtaac tggcaatctc ataatgctcg actttctgtg ctgcggcaat
    14701 cagtgcggca tcacgcactt cgtttttctc ggtactttcg atgacctcat tagcttcttc
    14761 aataagacct tccattgcca cacatttcat gcgcttaatt ttcagattcg attctgattc
    14821 cacaacttga tcaatacgtt caatctgtcc atatgtttcc tcgaggtgcg catgaaaagc
    14881 ctgacttaat ttttcgtttg atgttgctct tgcgagtttt gccagtgccc gggttaattg
    14941 tttttctgcg ctgtaggtat ctgaaagcag gtgaataaat acatcttcaa tggtcttcat
    15001 attcatattt ttctccagtg aaatcactgc gagccatacg gcccgcagat gcaattatct
    15061 ttatcagctg atattaataa ttaacggtga atcaggattt gcctgattta ttaccaccgc
    15121 tttgttgacc gcctttttta cccgcttcag atgcgcgttg cggatcattt ttaaaattac
    15181 cgccgctatg ctgaccgcct ttacggccag cgtcggatgc cttttcacgg tcttcggcga
    15241 aatttcctga accaccacga tgttcggcca tgttatttct cccgttgcgt tgcattgttt
    15301 cattaatatg ggtgttgtgt gtcgacactc attaaaatta gtcgctaatg aaaattagtc
    15361 aaattaagcg caacgagaaa aaaga
//
LOCUS       DAZKJR010000120        15196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKJR010000120 DAZKJR010000000
VERSION     DAZKJR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.121"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            234..679
                     /locus_tag="ACHOWS_003851"
                     /pseudo
     CDS             234..679
                     /locus_tag="ACHOWS_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            723..991
                     /locus_tag="ACHOWS_003852"
                     /pseudo
     CDS             723..991
                     /locus_tag="ACHOWS_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1196..2314
                     /gene="ybdK"
                     /locus_tag="ACHOWS_003853"
     CDS             1196..2314
                     /gene="ybdK"
                     /locus_tag="ACHOWS_003853"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFQ0049361.1"
                     /translation="MPIPDFQVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLMEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            2380..2628
                     /gene="ybdJ"
                     /locus_tag="ACHOWS_003854"
     CDS             2380..2628
                     /gene="ybdJ"
                     /locus_tag="ACHOWS_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFQ0049362.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2693..3061
                     /gene="ybdF"
                     /locus_tag="ACHOWS_003855"
     CDS             2693..3061
                     /gene="ybdF"
                     /locus_tag="ACHOWS_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0049363.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            3155..3808
                     /gene="nfsB"
                     /locus_tag="ACHOWS_003856"
     CDS             3155..3808
                     /gene="nfsB"
                     /locus_tag="ACHOWS_003856"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFQ0049364.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQVKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLEEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3916..5163
                     /gene="ybdG"
                     /locus_tag="ACHOWS_003857"
     CDS             3916..5163
                     /gene="ybdG"
                     /locus_tag="ACHOWS_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFQ0049365.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(5244..6620)
                     /gene="pheP"
                     /locus_tag="ACHOWS_003858"
     CDS             complement(5244..6620)
                     /gene="pheP"
                     /locus_tag="ACHOWS_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFQ0049366.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6722..9865)
                     /gene="cusA"
                     /locus_tag="ACHOWS_003859"
     CDS             complement(6722..9865)
                     /gene="cusA"
                     /locus_tag="ACHOWS_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFQ0049367.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGQTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9877..11100)
                     /gene="cusB"
                     /locus_tag="ACHOWS_003860"
     CDS             complement(9877..11100)
                     /gene="cusB"
                     /locus_tag="ACHOWS_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFQ0049368.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADESLKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(11116..11448)
                     /gene="cusF"
                     /locus_tag="ACHOWS_003861"
     CDS             complement(11116..11448)
                     /gene="cusF"
                     /locus_tag="ACHOWS_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFQ0049369.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKIIIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11472..12854)
                     /gene="cusC"
                     /locus_tag="ACHOWS_003862"
     CDS             complement(11472..12854)
                     /gene="cusC"
                     /locus_tag="ACHOWS_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFQ0049370.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSNNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            13011..13694
                     /gene="cusR"
                     /locus_tag="ACHOWS_003863"
     CDS             13011..13694
                     /gene="cusR"
                     /locus_tag="ACHOWS_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFQ0049371.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13684..15132
                     /gene="cusS"
                     /locus_tag="ACHOWS_003864"
     CDS             13684..15132
                     /gene="cusS"
                     /locus_tag="ACHOWS_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFQ0049372.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQAQRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFMIKLPEKG"
BASE COUNT         3684 a         4082 c         3729 g         3701 t
ORIGIN      
        1 atgatttaca cctaacacta taaaatgttc agaaagccat cgcaataata aaaaataaga
       61 cattacgata atattataaa gcattgcttt gttgggggat gacgtatgta gcaatgaaag
      121 ataacaaatg ccggtaatgg tacggagaaa tcctcagtaa taggcgggta aaaaagacgg
      181 gcatagagtt ttgtggtcga ctggttgtgc taacaattca ggagatcacc tctatgcccg
      241 ttcgctcatc atgccaaaag tttttccgtg atgatttgac tccatttcat cagtaccgcc
      301 agaatgccat ccttgacgcc actgctgcgc ttagtcggga cgcatccctc atccttacca
      361 gcattggacg gtatatgcca ggcaatgctc aggttaaaac aagattaagc ccatcgatcg
      421 cctgccaggc aatgacgctt tacaccgcga tatcccggct atttttcata atattacccg
      481 gtttcagccc cgagacctta cgttgtgcgt cattgccgtt gactggagtg gttatccttc
      541 tcaggcattc catgttctgc atactcccca agtttgcgac gggcgttcta tattacttct
      601 ggatcaggtt gtatcgtcat tctggcaaca aattgctcag ttctataaga attttcttga
      661 tacgcttgca atcactattg ctccgaataa accggataaa aattaccttt aaacacagca
      721 ccatgaccgc cacaagggca tatttcgtca gcatgtttat attgcctcca tgcaaagaag
      781 ggactaccat ccaaattgtt tgggtttgga gtggcagccc caggattgat agaaatatct
      841 cctggggctt catctttcca gacctcagag taaacctgaa accggaaagc ttcaggcacc
      901 cgccgttatc ttacccttcc ttagcaaaat ggctgtttat ttatacagta agtcctgttt
      961 cggacttctc aatcctcatt tgatcaactg aagctgaacc cgttttattc tgcatgcctc
     1021 aatcgtcaaa attcttcgcc accagtccgc actgccataa cttgtcatat tgcaaaatgt
     1081 agccaatttg cttcagtcac catcatcaaa aaaagaaaca ttccagaact tccgcatctc
     1141 gcccagccgc tccgacttct atactgaata gaaaacgcca acataagaga aacctatgcc
     1201 aatacccgat tttcaagtct ctgaaccttt taccctcggt attgaactgg aaatgcaggt
     1261 ggttaatccg ccgggctatg acttaagcca ggactcttca atgctgattg acgcggttaa
     1321 aaataagatc acggccggag aggtaaagca cgatatcacc gaaagtatgc tggagctggc
     1381 gacggatgtt tgccgtgata tcaaccaggc tgccgggcaa ttttcagcga tgcagaaagt
     1441 cgtattgcag gcagccgcag accatcatct ggaaatttgc ggcggtggca cgcacccgtt
     1501 tcagaaatgg cagcgtcagg aggtatgcga taacgaacgc tatcaacgaa cgctggaaaa
     1561 ctttggctat ctcatccagc aggcgaccgt ttttggtcag catgtccatg ttggctgcgc
     1621 cagtggcgat gatgccattt atttgctgca cggcctgtca cggtttgtgc cgcactttat
     1681 cgccctttcc gccgcgtcgc catatatgca aggaacggat acgcgttttg cctcctcacg
     1741 accgaatatt ttttccgcct ttcccgataa tggcccgatg ccgtgggtca gtaactggca
     1801 acaatttgaa gccctgtttc gctgcttgag ttacaccacg atgatcgaca gcattaaaga
     1861 tctgcactgg gatattcgcc ccagtcctca ttttggcacg gtggaggttc gggtaatgga
     1921 taccccgtta acccttagcc atgcggtaaa tatggcggga ttaattcagg ctactgccca
     1981 ctggttactg acagaacgcc cgttcaaaca tcaggagaaa gattacctgc tgtataaatt
     2041 caaccgtttc caggcctgtc gctatgggct ggaaggcgtc attaccgatc cgcatactgg
     2101 cgatcgtcgg tcactaatgg aagacacctt gcgtttactg gaaaaaatcg ccccttctgc
     2161 acataaaatt ggcgcatcga gcgcaataga agcactgcac cgccaggtcg tcagcggtct
     2221 gaatgaagcg cagctgatgc gcgatttcgt cgccgatggc ggctcgctga ttgggctggt
     2281 gaaaaagcat tgtgagatct gggctggtga ctaagccggg aattgcctta cagaagaata
     2341 tccctgacaa tggtgtttta acttacattt aacaatgtta tgaaacaccc tttagaaacc
     2401 ttgaccaccg cagcaggcat tttgctgatg gctttccttt cttgcctgct gctgcccgcc
     2461 cccgcactgg ggctgacgct ggcacaaaaa ctggtgacca tgttccatct gatggatctt
     2521 agccagcttt acactttatt gttttgtctg tggtttttag tgctgggcgc tattgagtat
     2581 tttgttctgc gctttatctg gcgacgctgg ttctcgctgg cggattaaac gtcgattatc
     2641 gccgtacttc gcggcatact ttgcttattc tccttcgcct aaaggaacgt ttatggataa
     2701 gcaatcactg cacgaaacgg cgaaacgcct ggcccttgag ttaccctttg tcgagctttg
     2761 ctggcctttt ggcccggagt tcgatgtttt taaaattggc ggcaagattt ttatgctgtc
     2821 gtcggagctg cgcggcgtcc cctttatcaa tctgaagtcc gatccacaaa aatccctgtt
     2881 aaatcagcaa atatacccga gcatcaagcc agggtatcac atgaataaaa agcactggat
     2941 ttcagtgtat cccggcgagg aaatctccga agcgttactt cgcgatctga tcaacgattc
     3001 gtggaatctg gtggttgatg gtctggctaa acgcgatcaa aaaagagtgc gtccaggcta
     3061 aagcggaaat ctatagcgca tttttctcgc ttaccatttc tcgttgaacc ttgtaatctg
     3121 ctggcacgca aaattacttt cacatggagt ctttatggat atcatttctg tcgccttaaa
     3181 gcgtcattcc actaaggcat ttgatgccag caaaaaacta accccggaac aggccgagca
     3241 ggtcaaaact ctcctgcaat acagcccatc cagtaccaac tcccagccgt ggcattttat
     3301 tgttgccagc acggaagaag gtaaagcgcg tgttgccaaa tccgctgccg gtaattacgt
     3361 gttcaacgag cgtaaaatgc ttgatgcctc gcacgtcgtg gtgttctgtg caaaaaccgc
     3421 gatggacgat gcctggctga agctggttgt tgaccaggaa gatgccgatg gccgctttgc
     3481 cacgccggaa gcgaaagccg cgaacgataa aggtcgcaag ttcttcgccg atatgcaccg
     3541 taaagatctg cacgatgatg cagagtggat ggcaaaacag gtttatctca acgtcggtaa
     3601 cttcctgctc ggcgtggcgg cgctgggtct ggacgcggtg cccatcgaag gttttgacgc
     3661 cgccatcctc gatgaagaat ttggtctgga agagaaaggc tacaccagtc tggtggttgt
     3721 tccggtgggt catcacagcg ttgaagattt taacgctacg ctgccgaaat ctcgtctgcc
     3781 gcaaaacatt accttaaccg aagtgtaatt ctcccttgcc gggcatctgc ccggctattt
     3841 cccctcagat tctcctgatt tgcataaccc tgtttcagcc gtcatcatag gctgctgttg
     3901 tataaaggag acgttatgca ggatttaata tcccaggttg aagatttagc gggtattgag
     3961 atcgatcaca ccacctcgat ggtgatgatt ttcggtatta tttttctgac cgccgtcgtg
     4021 gtgcatattt ttttgcactg ggtggtactg cggaccttcg aaaaacgcgc catcgccagt
     4081 tcacggcttt ggttgcaaat cattacccag aataaactct tccaccgttt agcttttacc
     4141 ctgcagggga ttatcgtcaa tattcaggcg gtattctggc tgcaaaaagg caccgaagcg
     4201 gcagatattc tgactacctg cgcgcagttg tggatcatga tgtatgcgct gctttcagtc
     4261 ttctcgttgc tggatgtgat tttgaatctg gcgcagaaat tcccggcggc atctcagtta
     4321 ccgctgaaag ggatatttca ggggattaaa ctgatcggcg cgattctggt cggcattttg
     4381 atgatctcgc tgctgattgg tcagtcgcca gcgattctga tcagcggtct tggtgcaatg
     4441 gctgccgtgc tgatgttggt atttaaagat ccgattcttg gtctggtggc aggtattcag
     4501 ctttccgcga acgatatgct gaaactgggc gactggctgg agatgccgaa atacggcgcg
     4561 gatggcgcgg tgatcgatat tgggttaacc accgtcaaag tacgtaactg ggacaatacc
     4621 attaccacta ttcccacctg gtctctggtt tctgactcct ttaaaaactg gagcgggatg
     4681 tcagcatctg gcgggcgacg tattaagcgc agtatcagta ttgatgtcac cagtattcgt
     4741 tttcttgatg aagacgaaat gcaacgtctg aataaagcgc atttgttaaa gccttattta
     4801 accagccgcc atcaggaaat taatgagtgg aatcgccagc aaggttctac ggagtcggta
     4861 ttaaacctgc gccgaatgac caatattgga acctttcgtg cctatctgaa cgaatatcta
     4921 cgtaaccatc cgcggattcg taaagatatg accttaatgg tacgccaact ggctcctggc
     4981 gataacggtt taccgctcga gatctatgcg tttaccaaca ccgtggtgtg gctggaatat
     5041 gaaagcattc aggctgatat attcgaccac atatttgcga ttgtcgaaga gtttggtctg
     5101 cgacttcatc agtcgccaac tggcaatgat attcgctctc tggcgggtgc atttaagcag
     5161 taattaaaaa aaccgctctc atcgaatgga tgagagcggt ttcggatggt tgacatcatt
     5221 ttgtcggata cggcgcgaat gccttatttc cgacgcagcg ttttaaacgc tacaaacagg
     5281 aatacaatcc acactggcag cagaatcgct gacaagcgca tatcatccat cgtgcacatc
     5341 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     5401 gccttaaact gtgtttcacg tccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     5461 aggcagatca taatccagtt caacagcagc gttgctacca ccagcgccat cagcagacca
     5521 aacgcttttt gcggcagcag atagttaatc agcaccacca gcgaagtgat ggctccggaa
     5581 agcatcagcg agttaatcgg cacgccgcga cggctgacgc gagtcaaaaa cttcggtgca
     5641 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     5701 accgacagcg atgccaccag aatgacgaag ttcagcgcag aagctaccac gttgctgtcg
     5761 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5821 agtgccagta aaaccaccag tgaaccgata taaaacagca gaatgcgata caccacctga
     5881 ttcactgctt tcggaatgct tttttccgga tcgcgcgctt cagcggcagt aatcccaatc
     5941 agctccagac cgccgaaaga gaacataatt accgccagcg acaaaatcag cccattccag
     6001 ccggtggcaa aaaaaccacc gtaacgccag aggttatcga tactggcttt ctcgccgccg
     6061 tgaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     6121 atcagcgcaa accagaattc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     6181 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     6241 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     6301 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     6361 aatgaaccgg ataccggctc ctcaaccacc atttcgccaa gctggcgcat aatcaggaaa
     6421 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     6481 ggaccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     6541 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     6601 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     6661 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacggg gtatcctgct
     6721 tttatttccg tacccgatgt cggtgcagcc acatcagctt atacgccgcc gggataataa
     6781 acagcgacag caaaggtgcg gtgatcatgc cgccaatcat cggtgcggca atccggctca
     6841 tcacctctga accagccccc gttccccaca gaatcggcag cagaccggcg ataatcactg
     6901 ccaccgtcat cgctttcggg cgcacgcgca ggaccgcgcc gtgatacaac gcctcatcca
     6961 gcttctgctc gctgaatgtt tgcggattat tcaacgacgg ctcggcctct atggcgtgac
     7021 gtaaatacat cagcatcacc acgccaaatt cggcggcaac cccggcaagg gcaataaaac
     7081 cggtgcccgt cgccacggaa agatgaaagc ccatccacca cagcaaccag atgccgccca
     7141 ccagcgcaaa cggtacgctg ctgataatca gcaacgcttc gcccacccga cggaacgcca
     7201 gatataacag cacgaagatg atcatcagcg tcatcggcac catgagtttc agcttatggt
     7261 tggcgcgctc cagtagctcg aactgcccgg agaatgccac gctggtgccc ggttttaact
     7321 gcactttttc agctatcgct ttttgcagat cgtgaaccac cgacaccatg tcacgatcgc
     7381 gggcatcgat ataaatccag ctcgtcgggc gcgcattctc ggttttcagc atcgacggtc
     7441 cggtagagac tttaacgtca gccacgtctg ccagggtgat ttgctgcttc atcggcgtga
     7501 ggatcggcaa ctggcgcagt gcctgcgggc tatcgcgcca gctttgcgga taacgcagat
     7561 taattggata acgagcaatc ccttccaccg tttcgccaac catcgccccg cccaccgcag
     7621 aagtcacaaa caactgcaca tccgccaccg tcataccgta acgcgcggct ttttcacggt
     7681 taatctcaac gttgatataa cgcccacctt ccagacgctc agcaagcgca gaagccacgc
     7741 ctggcaccgt tcgcgccact tcttcaattt gctccgccat cgcgtcgata tccgccagca
     7801 cggtgccgga aactttaata ccgatgggac ttttaatgcc agtcgagagc atatcgatac
     7861 ggttacgaat tggcggcacc cacagattcg ccagccccgg caaacgcacg gtgttatcca
     7921 gttcctcaat gattttgtcc atcgtcatgc caggccgcca ctgatcctgc ggcttaagct
     7981 gaatggtcgt ttctaccatc tccagcggag cagaatcggt ggcggtttcc gctttcccgg
     8041 tttggccaaa tacccgcgcc acttccggta cgctcataat cagcttgtcg gttttttgca
     8101 gcatactcgc cgcctccgct gcggaaatcc ccggcagcgt cgatggcata tacaacaagt
     8161 cgccttcatt gatctgcggt aaaaattccc cgccaacttt attgagcggc cagagaaccg
     8221 tcagcaccga aagcgctgcc accaacagcg tggttttcgg ccagtgcagt actttcagca
     8281 acagcggatg ataaacacga atcaaaaagc gattgagcgg gttactgctt tccggcggaa
     8341 ttttgccacg gatccagtag cccatcagga tcgggatcac tacgatcgcc agtagcgccg
     8401 cacccgccat cgcataggtt ttggtgaacg ccagcgggcc aaacagacgc ccttcctgcc
     8461 cttccagggt gaagatcggg ataaacgaca gcgtgataat cagcagactg ataaacagcg
     8521 ccggccccac ttcaacagat gcatcggtaa tcacctgcca gcgcgtttta ttatccaacg
     8581 tggcgtcagg atgctggtgc tgccactctt ccagccgttt atgcgcattc tcgatcatga
     8641 cgatagcggc atcgaccatc gccccgacgg caatcgcaat gccacccaac gacataatat
     8701 tggcgttcag tccctgaaag tgcatgacaa taaaagcaat acacaacccc agcggcaacg
     8761 aaataatcgc caccagcgcc gagcgcacat gccagagaaa cagcgcacag actaccgcca
     8821 ccacaataaa ctcttccagc aacttgccgc tgaggttgtc gatggcgcga tcaatgagct
     8881 ggctgcgatc gtatgttgta actatctcca cgccttccgg cagactactt ttcagcgttt
     8941 ccagtttgtc cttcacggcg gcgattactt ctcgggcgtt tttgccggag cgcaggatca
     9001 ccaccccgcc cgccacttcg ccttcgccgt tcagttcggc aatgccccgg cgcatctccg
     9061 ggccaatctg gaccttcgca acatcacgca gataaacggg cacgccattt tcactggctt
     9121 ttaaaacgat gtgattaaag tcgtcgagcg tttgcagata gccgctggct cgcaccatat
     9181 attccgcttc cgccagttcg atcgacgaac cacccgcttc ctggtttgaa gcatccagcg
     9241 cgctttttac ttcggcgaga ctgatgccat attgcgccag gcgctgagga tcgataacca
     9301 cctgatactc tttcaccaca ccgcctaccg acgccacttc cgcaacgtca ggaatggttt
     9361 tcagctcata tttgagaaac cagtcctgca atgagcgtaa atcggcaaga tcgtgcttac
     9421 cgctgcgatc caccagtgca tattcataga tccagccaac acccgtggca tctggcccca
     9481 gctcggcgct gactcccgca ggcagcttac cctgtacctg gttgaggtac tccagcaccc
     9541 gcgagcgcgc ccagtacgga tcggtgccat cttcgaaaat gacatacaca taagagtcgc
     9601 caaactgcga gaaaccgcgc acagtcttcg cgccaggcac cgacaacatg gtggtggtta
     9661 gcggataagt cacctgattt tcaacgattt gcggtgcctg accgggatag ctggttttaa
     9721 taatcacctg cacatcagag agatccggca gtgcatccac tggcgtgtta atgatggtcc
     9781 aggtgcccca gatactcaga aacaacgcgc ccatcagcac cagaaaacgg ttcgccaccg
     9841 agcgacgaat aatccattca atcattggtt attccctcaa tgcgcatggg tagcactttc
     9901 agagcgcatc cgctccagtg cgccagaaat attggcttcg gaatcaatca ggaacaggcc
     9961 gctggaaacc accttttcac cttccgccag accagagcgt aacgcggtga cgccttgcga
    10021 tgcctggaaa acggcaacgc gtttcggtac aaagcgtccg tcggcatcaa cggtaatcac
    10081 ccgctgttca ctgccggtat caatcagcgc ctgtgacgga atgagcagca tcggttcgct
    10141 ggcggtattg agttgcaacc aggcgttcat gcccggtttt agcgactcgt cggcgttgtc
    10201 gacttccaga cgcagctgca gcgtgcgggt cgcggcatcc acgccgggta gcagggtcca
    10261 tttgcgaatg gtgagtgttt tatcaggtcg cgccggaacg gtgagcgtaa actgcgaggc
    10321 atctttcacc agccatgcga tggactccgg gatcgcggca gtgatccaca ccgggtccat
    10381 accctgaatt ttcgcgacca cgttatcttt ggcgatattc attcccgcgc gcagatcaaa
    10441 cgcggtgatc acgccatcaa tgggtgcttt gagcgtaaag cgagtctgga ttttttgcgt
    10501 ggcaatcagg cggcgaatat ccgcctccgg cattcccgcc agtcgcagtc gctcaagaat
    10561 gccttcagtc tgggtcgccg taccgccggt ttcgcgcagc agtaaatact cactctgcgc
    10621 ttccacccag tcgggaatgg tcaggtcgag aagcggtgtg cccttttgta ctttatcgcc
    10681 cacggtaagc ggatacacct tatcgataaa cccggcagcg cgagcctgca caatggcata
    10741 ctgatactcg ttgtaactga cattcgccgg gaaactctgg gcaaaagtca gcggcccgcg
    10801 cgttacggtc gccgttttca cccccagatt ctgcgtctgg gtcgggtcaa tgcgtacacc
    10861 agacgcagaa ctctcttcat cggcatattt cggcaccaga tccatatcca taaacggcga
    10921 tttacctggt ttatcgaacc gcgtattggg atacatcggg tcgtaccaga ataagatttt
    10981 acgttctgcg gtcgacgttt tttctgcggg cggttccgcc tttgcaaacc aggtaaaacc
    11041 tgccgcagaa ataataccgc ccgcgatcat gctgccgata ataagcgcga tttttttcat
    11101 ctcattaaac ctgggttact ggctgacttt aatatcctgt aataaagaaa ggttgccctg
    11161 ctggacaaaa ttaaacgcca ctttgtcgcc ggttttaatt tcactcattt tcgtctgcgg
    11221 ggtgatggta aagcgcatgg tcatctccgg ccagttcacc gcggtaattg gatcgtggtg
    11281 gatgatgatt tttttgcttt ccagatcaat ggtttttacc acaccggtgg cgctaatgac
    11341 ctgtggttgt gcctcgctca tggtttcatg atgatgttcg ttagcctggg cattaaagcc
    11401 aataacggta aacagactaa acattgcgac ttgcagtgct tttttcattt attctctcct
    11461 ggagttaaaa attattgctg ccaaccgccg cctaaggcgg tatataaaga aatttcatta
    11521 acctgacggg cataattcag gtcaagtaaa gtttgtcggg ttgcaaataa agaacgctca
    11581 gcatccagca cttccagata acttaccgcg ccgtgctgat ataatgcccg cgcccgttgc
    11641 aaagtaattt gcagcgacgc cagataacgc tgctgggcgc taatttgatc gttcaggctt
    11701 tgacgtaatg ccagcgcatc tgccacttct ttaaaggcgt tctggatttt ctgttcataa
    11761 ttcaccaccg actgctgctg gcgaatttcg gcgatatcca gattggcctg gttacgtccg
    11821 gcattaaaaa tggggatctc aattttgggt ataaaattcc acatcccgct gctggcgtta
    11881 aataatgacg acagatcgct gctggcggtt gatattccgc tggtcaggct gatagacggg
    11941 aaaaatgccg cgcgtgcagc accaatattg gcattagccg ccattaacgc atgttcagct
    12001 tccataatat ccgggcgctg caataagatt tgcgacgaca agcccgccgg taatttaacg
    12061 ctttgcaggc tgtcgctgtt tactgtctgc gcttgcggca gcttgccgta gcttcccaat
    12121 aacagttgca atgcattatt cgcctgcgcc agttccccct gacgtttagc gatgtcgctg
    12181 cgggtacttt caatcacccc gcgagcctgt tccagcgcca gaacattact gctacctgtc
    12241 aacagttgtt tttcgacaaa cgcatatgac tgctgataat tacgcagcgt ttcttcggct
    12301 atttgcaatt gcgcatacgc cagctgctga ttgaaatagc tttgcgcgac attagaaacc
    12361 agcaaaatat gcaccgcgcg ctgagcttcc tcagtggcta aataattttg gcgctcggct
    12421 tcgctcatgt tctttaagcg cccgaaaaaa tcgagatcaa agctggcgtt aaggccagtc
    12481 gagaactccc gcgtcgtggc tgaatcgcct ttaagattgt tgctccagct gccgctgccc
    12541 tcgccattga gctgtgggta gcggtcggca tcggtcagac gatattgcgc ccgcgcttcc
    12601 tgcactttca gcgtcgccat gcgcaaatcc cggttattca ccagcgcctc gctaatcagc
    12661 gtcttcacct gattatcaac aaaaaaggtg cgccagcccg cgttctggta gttatctgct
    12721 gcgttaacca ggccgttctg gttgagtgag aactgctgcg gcacgggcat tgccgggcgc
    12781 tgataatccg gtgccagtga acaaccggtt agcgcaaggg ccacacaaaa tggcagaagt
    12841 ttacaaggag acataggctc ataatttctg gtgattttat gccgccaact ttactcgcca
    12901 ggctctgatt ttccggtgac aggaaaatga caaaattgtc attttgccaa taagcgattg
    12961 ccatctgatc ccgctactct agaattgccc gggcaacatg cggaggaaat atgaaactgt
    13021 tgattgtcga agatgaaaag aaaaccggag aatacttgac caaagggtta accgaagccg
    13081 gttttgtggt cgatttggcc gacaacgggc tgaatggcta ccatctggcg atgaccggtg
    13141 attatgatct gataatcctc gatattatgc tgccggacgt gaacggctgg gatatcgtgc
    13201 gcatgttgcg ttccgccaat aaggggatgc cgattctgtt gcttaccgcg cttggcacca
    13261 ttgaacatcg cgtcaagggg ctggagttgg gggcggatga ctatctggtg aagccgttcg
    13321 cttttgctga actgctggcg cgagtgcgca ctctactgcg gcgcggggcg gcggtgatta
    13381 tcgaaagtca gtttcaggtt gccgatctga tggtcgatct cgtcagccgc aaagtcaccc
    13441 gcagtggcac gcgcatcact ctgaccagta aagaatttac tctgctggag tttttccttc
    13501 gccatcaggg cgaagtactg ccccgctcac ttattgcctc acaggtgtgg gacatgaatt
    13561 tcgacagcga cactaacgcc attgatgtgg cggtgaagcg gctgcgtggc aaaatcgaca
    13621 acgactttga gccgaagctg attcaaaccg tgcgcggcgt gggttacatg cttgaggtgc
    13681 cggatggtca gtaagccgtt tcagcgcccg ttttcgctgg caacccgcct gacctttttt
    13741 attagcctgg ccaccatcgc ggcgtttttc gcctttgcat ggatcatgat ccactcagta
    13801 aaagtgcatt ttgccgagca ggatattaat gatttaaaag agattagcgc cactcttgaa
    13861 cgggtactta atcaccctga cgaaacgcaa gcccaacgct taatgacgct ggaagatatc
    13921 gtcagtggtt attccaatgt gttgatttcc ctggcagata gccacggtaa aacggtgtat
    13981 cactcccccg gtgcgccgga tatccgcgag tttacgcgtg acgccatacc cgataaagac
    14041 acgcggggcg gtgaggtgta tctcctttcc ggcccgacga tgatgatgcc gggccacggt
    14101 cacgggcata tggaacacag caactggcgg atgattagct tgccggttgg cccgttggtg
    14161 gacggcaaac cgatttatac gctctacatt gcgctttcga tcgattttca tcttcattac
    14221 ataaatgatt tgatgaataa acttattatg accgcatcga tcatcagcat cctgatcgtc
    14281 tttatcgtgc tgttggcggt acataaaggt cacgcgccga tccgcagcgt cagccgtcaa
    14341 atccagaaca ttacctcgaa agatctcgac gttcgccttg acccgcagac cgtgcccatt
    14401 gagctggaac agctggtact gtcgttcaac catatgatcg agcgtattga ggatgtcttt
    14461 acccgccagt ccaatttctc agcggacatc gcccacgaaa ttcgtacacc aattacgaat
    14521 ctcataacgc aaacggaaat cgccctcagc cagtcacgca gccagaagga gctggaagat
    14581 gtgctctact ctaatctcga agagctgacg cgaatggcga aaatggtcag cgatatgctg
    14641 tttctcgccc aggccgataa caaccagtta atccccgaaa agaaaatgct caacctggcg
    14701 gatgaagtcg gcaaagtgtt cgattttttc gaggcattag cggaagatcg cggcgtggag
    14761 ttgcggtttg ttggtgacga gtgtcaggtt gcgggcgatc cgctgatgct gcgtcgggcg
    14821 ttaagcaacc tgctttcgaa cgccctgcgt tatacgccac ccggagaggc aattgttgtg
    14881 cgctgccaaa cggtcgatca ccaggtgcaa gttaccgtcg aaaaccccgg tacgcccatt
    14941 gcgcccgagc acttaccgcg attgtttgac cgtttctatc gcgttgaccc ctcccgtcag
    15001 cgaaaaggcg aaggtagcgg tattggcctg gcgatagtga aatcgattgt tgtcgcgcat
    15061 aaaggcacgg ttgcagtaac gtcagatgct cgggggacaa ggtttatgat caaactaccg
    15121 gagaaaggtt aattagttag ttcaaactca aagagtgatg tatcgccttc tgttattatt
    15181 tatcaataaa acaggg
//
LOCUS       DAZKJR010000121        15181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKJR010000121 DAZKJR010000000
VERSION     DAZKJR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.122"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(277..352)
                     /locus_tag="ACHOWS_003865"
     tRNA            complement(277..352)
                     /locus_tag="ACHOWS_003865"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Phe,seq:gaa)
     gene            complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHOWS_003866"
     CDS             complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHOWS_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0049373.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1318..1395
                     /locus_tag="ACHOWS_003867"
     CDS             1318..1395
                     /locus_tag="ACHOWS_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049374.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            1558..3693
                     /gene="speC"
                     /locus_tag="ACHOWS_003868"
     CDS             1558..3693
                     /gene="speC"
                     /locus_tag="ACHOWS_003868"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFQ0049375.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAAEQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLFGYVLK"
     gene            complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHOWS_003869"
     CDS             complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHOWS_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFQ0049376.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHOWS_003870"
     CDS             complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHOWS_003870"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFQ0049377.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHOWS_003871"
     CDS             complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHOWS_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFQ0049378.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHOWS_003872"
     CDS             complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHOWS_003872"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0049379.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7861..8580
                     /gene="trmB"
                     /locus_tag="ACHOWS_003873"
     CDS             7861..8580
                     /gene="trmB"
                     /locus_tag="ACHOWS_003873"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0049380.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8580..8906
                     /gene="yggL"
                     /locus_tag="ACHOWS_003874"
     CDS             8580..8906
                     /gene="yggL"
                     /locus_tag="ACHOWS_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFQ0049381.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            9090..9809
                     /gene="yggN"
                     /locus_tag="ACHOWS_003875"
     CDS             9090..9809
                     /gene="yggN"
                     /locus_tag="ACHOWS_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFQ0049382.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9985..11031
                     /gene="ansB"
                     /locus_tag="ACHOWS_003876"
     CDS             9985..11031
                     /gene="ansB"
                     /locus_tag="ACHOWS_003876"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFQ0049383.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11148..12155
                     /gene="yggM"
                     /locus_tag="ACHOWS_003877"
     CDS             11148..12155
                     /gene="yggM"
                     /locus_tag="ACHOWS_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFQ0049384.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSADCYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQTNERWKKDTEQLRRNGVI
                     K"
     gene            complement(12211..13512)
                     /locus_tag="ACHOWS_003878"
     CDS             complement(12211..13512)
                     /locus_tag="ACHOWS_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0049385.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(13512..14015)
                     /locus_tag="ACHOWS_003879"
     CDS             complement(13512..14015)
                     /locus_tag="ACHOWS_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0049386.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEVS"
     gene            complement(14060..15046)
                     /locus_tag="ACHOWS_003880"
                     /pseudo
     CDS             complement(14060..15046)
                     /locus_tag="ACHOWS_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         4023 a         3641 c         3849 g         3668 t
ORIGIN      
        1 cagacactgg aagatgccct gagtttgtat acagatgagc gcacagtgag caagatttgc
       61 aaagggggga ataaatcttg acaggctgaa tcagcaatgt aagcatacag tgttaacgag
      121 aacagggcgt ctctgcaaaa gacaccctgt ttgaggatac gataccgaac tcaatcagtg
      181 taaccacaac ttacacaaac ggagaccctt tgatgccgca tcctgtatta accgcccgtc
      241 aaggatctca tcccttaacc agcgtgttct ggtatatggt gcccggaggc ggaatcgaac
      301 caccgacacg gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc
      361 gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc
      421 acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg
      481 cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt
      541 taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt
      601 ctgccatcat cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta
      661 actggatgat cagtaatggg atactaatta ccgacaccgc aatacccaac gagcaggcga
      721 agatcatcgc cgtaaagaaa tcgagaaatg acttggcgat taaaatactc ggatcgccgg
      781 tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa
      841 tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag tggcgaaaca
      901 gattttgtgc tttggtgacc gcggtattga cgcctttttc cagcagacaa atttcgccga
      961 ttaatgcacc gagtaaggtt gctaaaacca tcgccggaag gttggcgcat ttcaccacca
     1021 gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac
     1081 gttccggtaa acgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag
     1141 cgttgataaa agggccgatg accacaagag ttcctgttaa ctgtattgtc tgattttctt
     1201 attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgct
     1261 ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg
     1321 cacgccacct ccttacattc tctcgcttat cgccgtttcg cgcgaaactc ctcccttttt
     1381 ctgctcatgc ggtgaagtta acgcacgctc actgcaggac aacagtaaaa tcagagcgtt
     1441 tctgctttta ctgatgtctg gcggtcggag ctggtgacca gtttgaccca catctcatgg
     1501 ggcaggtttg tccatcctgt ccggtactct tacttccccg aaacgggttt tgcgcttatg
     1561 aaatcaatga atattgccgc cagtagtgaa ctggtatccc gactttctac tcatcgccgc
     1621 gtggtggcgt tgggagatac tgattttacg gacgtcgcgg cagtcgtcat taccgctgcg
     1681 gatagtcgca gtggcattct tgcgttgctt aagcgcaccg gttttcatct accggtgttt
     1741 ttgtattccg aacatgttgt tgaattacct gcgggcgtta cggcggtaat caacggcaac
     1801 gagcagcagt ggctggagct ggaatccgca gcctgtcagt atgaagagaa tttgctgcca
     1861 ccgttttatg acacgctgac gcagtacgtt gagatgggca acagcacctt tgcttgccct
     1921 ggacatcaac atggtgcgtt ttttaaaaag catcctgccg gacgccattt ttacgatttc
     1981 tttggtgaga acgtctttcg cgccgatatg tgtaacgctg acgtaaaatt gggcgatctg
     2041 cttattcatg aaggttcggc gaaggatgca cagaaattcg cagccaaagt ctttcatgcc
     2101 gataaaacct attttgtgct gaacggcaca tcggcagcga ataaagtggt gacgaatgcg
     2161 ctgttaacgc gtggcgatct ggtgctcttc gaccgtaaca accataagtc gaatcatcat
     2221 ggcgcgttga ttcaggcggg ggcgacgccg gtctatctgg aagcttcacg caacccgttt
     2281 ggtttcattg gtggtattga tgcgcactgt tttaatgaag agtatctgcg ccagcaaatt
     2341 cgcgacgttg cgccagaaaa agcagacctg ccgcgtccgt ttcgtctggc gattattcag
     2401 ctgggaacct atgacggcac tgtctataac gcccgtcagg tgatcgatac cgttgggcat
     2461 ctgtgtgatt acattctgtt tgattccgcg tgggtcggtt acgaacagtt tatcccgatg
     2521 atggcggata gctcgccgct gctgttagaa cttaacgaaa acgatccggg gatctttgtg
     2581 acgcaatcgg tgcataaaca gcaggcggga ttctcacaga cgtcgcagat ccataaaaaa
     2641 gataaccata ttcgcgggca ggcgcgtttt tgccctcata agcggttaaa taatgccttt
     2701 atgctccatg cttctaccag cccgttctat ccgctgtttg ccgcgctgga cgttaacgcc
     2761 aaaattcatg aaggggagag tgggcgtcgg ctgtgggcgg agtgcgttgc gttggggatt
     2821 gaagcccgga aggcgattct tgcgcgctgt aagctgttcc gcccgtttat tccacccgtt
     2881 gttgatggca aattgtggca ggattatccg acgtcagtgt tagccagcga ccgtcgtttt
     2941 ttcagttttg agccgggggc gaagtggcac ggctttgaag gatatgccgc ggagcagtat
     3001 tttgtcgatc cgtgcaagct gttactgacc acaccaggta ttgatgctga aaccggtgaa
     3061 tatagcgact ttggcgttcc ggcgacgatt ctggcgcatt atctgcgtga gaacggtatt
     3121 gtgccggaga agtgcgatct caactccatt ctgttcttat taacgccggc ggaaagccac
     3181 gagaagctgg cacaactggt ggcgatgctg gcgcagtttg aacagcatat tgaggatgac
     3241 tcgccgctgg cggaggtgtt gccgagcgtt tataacaaat atccggtgcg ctatcgcgac
     3301 tacactttgc gccagttgtg tcaggagatg cacgatctgt atgtcagttt cgacgtcaaa
     3361 gacctacaaa aagcgatgtt ccgccagcag agtttcccgt cagtggtgat gaacccccag
     3421 gatgcgcata gcgcttatat tcgcggtgaa gtggagttgg tgcggattcg tgatgccgaa
     3481 gggcgaattg cggcagaagg ggcgttgcct tatccaccag gcgtgctttg cgtggtaccc
     3541 ggagaagtct ggggtggggc ggttcagcgt tatttccttg cgctggaaga aggggtgaat
     3601 ttattgccgg gattttcgcc ggagctgcaa ggtgtttata gcgaaaccga tgcggatggc
     3661 atgaaacggt tgttcggtta tgtgttgaag taagaataaa aaaaacgggt caccttctgg
     3721 cgacccgttt ttctttgcgt aattagtggc taaccgtctg tgtgcctgtc gggacacgaa
     3781 cgtgtttata tttgaacatc gccatgaacg cgaaggccag aaccacggag taaccagcga
     3841 aaatcagcca tacggtctgc cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt
     3901 taccgctcac gatgccgccg aggatacagc cgaagccgtt agtcatcatc aggaacatcc
     3961 cctgcgcact ggcgcgaatt gccgggctaa cttctttttc gacaaacacc gaaccagaga
     4021 tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga cagtaccagc agtacagtac
     4081 cgaacggagt cgggtcgccg taagcaaaca gcgcaaaacg caggatccac gccacaatac
     4141 tgatcatcat tacgttctta ataccgtagc ggcttaagaa gaacgggatg gtcagaatga
     4201 acagggtttc agagatctgc gaaatcgaca tgatgattga cgcatgctgc acgatgaagc
     4261 tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa ggtgttaccg aacatgttgg
     4321 taatctgcag ttccgcgccc agcagcattg agaagatgaa gaagattgcc atacgcttgt
     4381 ttttaaacag cgcgaatgca tcgaggccca gcagggttgt ccagctctga ttcgcttgct
     4441 gtttagcaac cggaatatgc ggcagagtca gggtaaacag aaccagaatg gcggaaagtg
     4501 ctgcgccaat atacagctgc atgtggctta attcgaagcc agacaggctc accacccaca
     4561 ttgccatgat aaagccgatg gtgccccaga tacggattgg cgggaagtca gtaacgatat
     4621 ccatcccggc attttgcagg cgatagtaag agatggtgtt gattaaccca agcgttggca
     4681 tataagcaaa cgagttaatc aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg
     4741 ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat ggcatatacc catttcgcac
     4801 ttaaccattt gtcggccaca atccccagca gcgcaggcat aaataccgcg gcgataccca
     4861 gtgagctata aactgcgcca atagaagcac cgtcaaactt cagggtaaca aacatatagg
     4921 agccgagggt cgtcagccaa cttccccaca gacagaactg cagaaaagag aggattttca
     4981 gctgcagctt aagattcatg ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa
     5041 agcacatttg accgtgcgag atgatgggat tctatcaact tcccggactg gttttttgtt
     5101 acctgtggca aataattgca aacattccca atttgcaaaa cgaaattgtg atgtggataa
     5161 catttttacc cctgagcatc gtcaggggcg gttagtggaa ttatcggcgg cggtaggatt
     5221 tttgcgcggt attcacttta taaagataac ggcgagattc cgcagaggga tggcgggtcg
     5281 tcagcgtttg ataaacatcg cccggcgtca tggtgttaat aatattggcc gcctgaatct
     5341 tatcattcga aaagactcgc agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg
     5401 cataacgccg ggatgtaggg ttatcaattc cgccgagata aacgttgttc agcatcgcca
     5461 gatacgcggt gccggtatca atattgctgg caggatcaaa caagaaactg cggctcggcg
     5521 tgccggattt cccctgcgaa cggaacacat ctttcccggc agtatgttgt accacctgca
     5581 ttaaccccag cgcatcggaa cggctgaccg catacgggtt aaaggatgac tcggtctgca
     5641 taatcgccag aatcagcgac tcatcaacgc catatttccg tgacgcctgg cggaccatgc
     5701 cgagatattt gtgcgcacgt ttatcaaggt ggttcggcac catgttaatg gtgacgctgt
     5761 agatgatacg cagcccgttg ctgcggctct tcagacggtt tttcagcaga taatccgcga
     5821 agttgctcgc gcgaccttcc cagcgaatcg gctgcccggt gttgtccacc acctgaccgt
     5881 aaaggaaagg ttctttcgaa atcgtaatat catcaacgtc ggaatagaga tcgaccgaac
     5941 tcggatcgtc ccccatcagc aacgttttga taattgcccg gcgcagatgc gcggcaggtt
     6001 ctgtcccggc gatggtttcg atagtaatcg taccgtcatc gaagttgatg tggctgcggg
     6061 tctgatattg atcggtgtat ttcacgtagt ccttaggacc ggcgatcacc acctctttga
     6121 agccccagat gttctcaata ttgtgggcaa attgccccat cagaatatca aaaccgttgg
     6181 tatctttgac ccaggcttcg ttataggtat cgcctttttt ggtcgtcgaa caggagatga
     6241 gcaacggcgc aatcaaagcc agcgcgagat attttttcat cattccgggt gcgtgttgtg
     6301 tttgttttta tgcaagttgc cggaggcgtg ctccggcact gtttttattt tttatcttcc
     6361 ggcgtatagc cctcaatatg cacctcttta ccctcgaaca ggaagttgac catctcctgt
     6421 tccagcagct tgcggtgctc ggcattcatc atgttgagtt tcttttcatt aatcagcatg
     6481 gtttgtttgt gctgccactg cgcccaggct tctttggaga tctcgttata gatgcgtttt
     6541 cccagctcgc cggggtacag ctgaaaatcc tgaccttctg cttcacgttg caggaaagta
     6601 caaaaaatcg ttctgctcat aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg
     6661 ccagtgcgta actgctgtaa caaacgctcc acgggagccg ccaggccaac tgacggcggt
     6721 tgcgctaagt tataccagag cgcattgcct tcatccatgc agccggtgaa tgacgacacg
     6781 ggaagccaca taggcacaat atctaagtgg aaatggctga aggtatgccg aaacgcggtc
     6841 agctgcgtca ggttatcggc agcaatctgc cgttgcgcca gccactgccg caaactttct
     6901 tcgtcggcaa actgcgggaa acagtataaa ccgccccaca atccgctcgg cggacgctgc
     6961 gccagcaata cttcatcttc gtgctgtaac aggagaaagt agccggtgcg ctccggcagc
     7021 gtctgtttcg gttttttgcc cggataaagc gaccagctat tgttggtggc ggcaatacat
     7081 ccgttttgta gcggacagag cgagcatttc ggcttcgagc gcgtgcaaat catcgcaccc
     7141 aaatccatca tcgcctgatt aaaccgctcc acgccgaccg cgggcgtcac ctgttcgctc
     7201 aaactccaca gtttattctc gacctctttt ttcccaggcc agccgcttac agcatagcag
     7261 cgcgccagca cccgtttgac gttaccgtcg agaatcggaa agtgcttacc cagagaaagc
     7321 gagagaattg cgcctgcggt ggaacgcccg acgcccggta acgccgcgac ttcttcaaag
     7381 gtttccggga atttaccgcc gtgtaaggtc gccacttgtt gtgccgcctt atgcagattg
     7441 cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga gaacttcgtc gagcggcgca
     7501 ttggcgagat cggtcaccgt cgggaagcgc gccataaagc gttcaaaata ggggataacg
     7561 gtcgcaacct gagtttgttg caacatcact tctgagagcc atactttgta gggcgtcttg
     7621 tcaatttgcc agggcagcgt tttccgcccg tatttatcgt accagtccag aacctgggct
     7681 gaaaattgcg acgcttgcat ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca
     7741 cagcgacggc agggtgtaaa ccggaacttt ccgcagccac ggccttatca tgcttgcatc
     7801 cggcaactaa ctttggataa tgcccgtttt cagaacactt tcacaagcga ctaaatcttt
     7861 atgaaaaacg acgtcatctc accggaattt gatgaaaacg gccgcccact gcgccgtatc
     7921 cgtagttttg tgcgccgcca ggggcgactg accaaaggcc aggaacatgc gctggaaaac
     7981 tactggccgg tgatgggcgt tgagttcagc gaagatatgc tggatttccc cgcgcttttt
     8041 ggccgagaag cgccggtgac gcttgagatt ggtttcggca tgggggcgtc gctggtggca
     8101 atggctaaag atcgccccga gcaggacttc ctcggcattg aagtgcattc accgggcgtt
     8161 ggtgcgtgcc tggcttctgc gcatgaagag ggcttaagca acctgcgcgt gatgtgtcac
     8221 gatgcggttg aagtgctgca taaaatgatt cctgacaatt cattgcgcat ggtgcagctc
     8281 tttttccctg acccgtggca caaagcgcgc cataataaac gccgtatcgt tcaggtgccg
     8341 tttgccgaac tggtaaaaag caaactgcaa ctggggggcg tattccatat ggcgaccgac
     8401 tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac
     8461 ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac gtccggtgac gaaatttgaa
     8521 caacgtggtc atcgtcttgg tcacggagta tgggacttaa tgttcgagag ggtgaaataa
     8581 tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat
     8641 taggattttc ggtggcatgg cgattcccgg aaggtacatc ggaagaacag attgataaaa
     8701 ccgttgatga ctttattaac gaggttatcg aaccgaacaa actggccttt gacggcagcg
     8761 gttatctggc ctgggaaggt ctgatctgca tgcaggaaat cggcaaatgc accgaagaac
     8821 atcaggcgat tgtgcgtaag tggctggaag agcgcaaact gggcgaggta cgcaccagcg
     8881 aacttttcga cgtttggtgg gactaagaaa gcatacgggc gatgacaaat gcaaaactgc
     8941 ctgatgtgct acgcttatca ggcctataaa gatgcacgat cgagtaggct ggataaggcg
     9001 tttacgccgc atccggcatg gaaaacgcgc actttgttat caatctgggg ccagcaaatg
     9061 ctggcctgat ttgttcttga gggaagacta tgatgcgcaa aatgctgctg gcggcagcac
     9121 tttcagtgac ggcaatgacc gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg
     9181 atgtgattgt cagcccgcaa accgtgcagg tgaagggcga aaacggcaat ctggtgatca
     9241 cgccagacgg caacgtgatg tataacggta agcaatattc cctgaatgcc gcccagcgcg
     9301 agcaggcgaa ggattatcag gctgaactcc gtagcaccct gccgtggatt gatgaaggcg
     9361 cgaaaagccg cgtcgaaaaa gcccgtattg cgctggataa aattatcgtt caggagatgg
     9421 gcgaaagcag caaaatgcgc agccgtctga ccaaacttga tgcgcagctg aaagagcaga
     9481 tgaaccgcat tatcgaaacg cgcagcgatg gcctgacgtt tcactataaa gccattgatc
     9541 aggttcgcgc cgaaggccag caattagtga atcaggcaat gggtggaatt ttacaggaca
     9601 gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg
     9661 tgctgggaag cctgggcggt ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa
     9721 aagatttcca gcagtttggc aaagatgttt gtagccgcgt tgtgactctg gaagatagcc
     9781 gcaaagccct ggtcgggaat ttaaaataat cctctatttt aagacggcat aatacttttt
     9841 tatgccgttt aattcttcgt tttgttacct gcctctaact ttgtagatct ccaaaatata
     9901 ttcacgttgt aaattgttta acgtcaaatt tcccatacaa agctaaggga taatgcgtag
     9961 cgttcacgta actggaggaa tgaaatggag tttttcaaaa agacggcact tgccgcactg
    10021 gttatgggtt ttagtggtgc agcattggca ttacccaata tcaccatttt agcaactggc
    10081 gggaccattg ccggtggtgg tgactccgca accaaatcta actacacagc gggtaaagtt
    10141 ggcgtagaaa atctggttaa tgcggtgccg caactgaagg acattgcgaa cgttaaaggc
    10201 gagcaggtag tgaatattgg ctcccaggac atgaacgatg atgtctggct gacactggcg
    10261 aaaaaaatta acaccgactg cgataaaacc gacggcttcg tcattaccca cggtaccgac
    10321 acgatggaag aaaccgctta cttcctcgac ctgacggtga aatgcgacaa accggtggtg
    10381 atggtcggcg caatgcgccc gtccacgtcc atgagcgcag acggtccatt caacctgtat
    10441 aacgcggtag tgaccgcagc tgataaagcc tccgctaatc gtggcgtgct ggtggtaatg
    10501 aacgacaccg tactggacgg tcgcgatgtc accaaaacca ataccaccga cgtagcgacc
    10561 ttcaagtctg ttaactacgg tcctctggga tacattcaca acggtaagat tgactaccaa
    10621 cgtaccccgg cacgtaagca caccagcgat acgccgttcg atatctctaa gctgaatgag
    10681 ctgccgaaag tcggcatcgt ttataactac gctaacgcat ccgatcttcc ggctaaagca
    10741 ctggtagatg cgggctatga tggcattgtt agcgctggtg tgggtaacgg caacctgtat
    10801 aaaaccgtat ttgacactct ggcgaccgcc gcgaaaaacg gcactgcagt cgtgcgttct
    10861 tcccgcgtac cgacgggcgc taccactcag gatgccgaag tggatgatgc gaaatatggc
    10921 ttcgtggcct ctggcacgct gaacccgcaa aaagcgcgcg ttctgctgca actggctctg
    10981 acgcaaacca aagatccgca gcagatccag cagatcttca atcagtacta atcacctcgc
    11041 cccggtaacg tgccggggct ttttcacttc agacttacgt ccattgccaa ttttcattac
    11101 cctgatgata atcactggaa taaattattc cgcgcgaggg ttttcgggtg aaaaagcaat
    11161 ggattgttgg cacggcgctg cttatgttga tgactggtaa tgtctgggca gatggcgaac
    11221 cgccaactga aaatatctta aaagatcaat tcaaaaagca gtatcacggc attctcaagc
    11281 ttgatgtcat caccttaaaa aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg
    11341 aaggcgatgt ctcttccagt gacgatctct atacctgggt cggtcagttg gcagattacg
    11401 aactgctcga acagacctgg acgaaagata aaccgataaa attctcggcg atgttaacca
    11461 gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta ctcttttcag gcggcagcca
    11521 gcgatcgtgg acgggtggtc gacgatatca aaacgaataa taaatatctg atcgtgaata
    11581 gcgaagattt caattatcgc tttagtcagc ttgaatcagc gttgaataac cagaagaact
    11641 cgattcctgc gctggaaaag gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga
    11701 aagcagcgga tgcatattgg ggaaaagatg caaacggtaa gcaaatgacc cgcgaagacg
    11761 cctttaaaaa aattcaccaa cagcgtgatg agtttaataa acagaacgat agcgaggcgt
    11821 tcgcggttaa atatgacaaa gaggtttatc aaccggcgat tgcggcatgt cataaacaga
    11881 gtgcagattg ttatgaagtg ccgattcagc agaagcgaga tttcgatatc aacgaacaac
    11941 ggcgacagac ttttctgcaa tcacaaaaac ttagccgtaa attgcaggat gactggataa
    12001 cgctcgaaaa agggcaatat ccgttaacga tgaaagtttc agaaataaac agtaaaaaag
    12061 tcgcgatttt gatgaaaatt gatgatatca accagactaa tgaacgctgg aaaaaagata
    12121 ccgaacaatt gcggcgcaat ggagtgatta agtaactcat gttaagtacc tttgccggat
    12181 aatataaaaa gttatccggc aaattatttt ttacattagc cctagtagct gtggaatgaa
    12241 caaggatatt tgcgggatgt aagtgatcat catcaacgcg attaacaatg cggcataaaa
    12301 tggcagcaat ggtttgatca aatgttgaat cttcacacct gaaatagaac aaccaacaaa
    12361 tagcgcacta cccaccggcg gtgttaacag cccaatacaa aggttagcca ccatcataat
    12421 accgaaatgt accggatcca ttcccagctc ttgtgcaatc ggcaagaaga ttggtgtgaa
    12481 gattagtaca gccggtgtca tgtccataaa gatgccgaca attaataaga cgatattaat
    12541 cagcagcaat atgaccagtg ggttatcaga aatccccatc aacgcgtcgc tgatcatata
    12601 aggaatatca gcattggtca ttgcccagga catgcccacg gaaaaaccga tcaacagtaa
    12661 tacaatcgaa gtggtaacaa ccgattccag aatcagtttg ggtaaatcgc gccattttac
    12721 ttcccggtaa atcagcaccg agagaataaa tgtgtagaca acggcaattg ctgacgcttc
    12781 agttgcggta aaaatgccgc ccagaatgcc gcccattaca atgaacacca gaagcaaact
    12841 tggtaaggca tccagaaacg ctttacatgc ctgagcaaag gttgctcttt ctgaaagtgg
    12901 gtacccacgg cgtttagcaa taatgccgca gactatcatt accgcgagac ccattaagat
    12961 ccctggcaaa tatcccgcca taaatagcga tgctacagaa acgccgccag ctgtcaggga
    13021 aaacacaatt aacacgtttg agggcggtat cagtaatcca gtaatacaag atgagacgtt
    13081 tacagcggta gaaaatgccg gatcataacc ttctttggtc tgaatgggat tcagtgtgcc
    13141 accaacggca gcagctgccg caacggctga accagaaatg gaaccaaaca tcatattcgc
    13201 caggacgttc acatgaccaa gcgaaccagg aacgcggcca accattacct gggcgaggtt
    13261 aatcaaacgt atcgcaatac cgccgctgtt catcagcgtt ccggcaaaga tgaaaaaggg
    13321 gattgccaat agggcaaagt tatccagtcc attagccagt cgctgcgaaa cagtgattgc
    13381 cgcgatatcc cagggaaaca tcaacagcat tgaagctaca gtggcaatac cgattgagaa
    13441 cgaaatgggc acaccaataa agaccagcac aaaaaaacag ccaaacagtg taagcgcgat
    13501 ataactatcc attaactgac ctcctgcgca ggttgtttca gttgttgaat gctttgatta
    13561 ataaaacaca aggcataaaa catcatcacc aatccactta atggcagaat gatatatacg
    13621 tatcccatcg gtatttgcat cgcggcagat acctgtgatg ttgccagtgt tttatcaatt
    13681 aatttcaggc caccagtcac aatgaccgaa ccggcaaaac ctaaaataag aacgttaatg
    13741 actatactaa gaagtaattg cttgcgctta ttcagggcga gagcaaataa atcaatagag
    13801 agatgtctct gcgcgccgac ggtatatgca gcacctaata atcctaccca gatcatcaaa
    13861 aagcgtgcca gctcatcggt taatgtactt ggctggttaa gtacgtagcg gctaaatact
    13921 tgccagacta cgcatacaac cagagcaagc attacggcga cagaaaacgc ggcaatgacg
    13981 cggtctaccg caagtttcag gcggttaaat atcattgtaa gcatcctgga aaatcagggc
    14041 cagagactgg cccgaaatat tattgagcag cgttgtcgaa tttctctaac aaagcagctt
    14101 gttttggatc ttttttgaag tcgtcaaaca gaggctgaac tgcggcacgg aatggggctt
    14161 tatcaacctt aacgatttca ccacccatgg ctttcgcctg agcacgtgtg tcagcgtcaa
    14221 ttttttccca caatttttgt tgataagctt cggatttctt cgccgctgtt tcgaggattt
    14281 gttgctgttc tggggttaat ttattccatg ttttggtgga gataaccaaa aagtcaggaa
    14341 ttgaggcatg ttcatcttca gaaaagactt tggcaatttc gatatgtcga gtctgcaccc
    14401 aggaaggcac gttattttct gcgccatcta ctacaccttg ctgcatcgca gtataaactt
    14461 cgccaaatga aattggagtg ggtgagccac ccatcagttc aatcattttg atggtggtcg
    14521 ggcttggctg aacacgaatt ttcagccctt taagatcttc tggcttagta atcggttttt
    14581 tcgcatagaa actgcgtgta cccgctacat aagctgataa agcaaaaaag cctttatctt
    14641 ttgttgaatc cataatttct ttaccaactt caccaaaaac cactttgttg aagtgcgcct
    14701 gatctttaaa taagaacggc aaattataga tagcataaat attatcaaaa gactccagat
    14761 cactggcgga acctttggtc atatccagtg caccattttg caacaactac atagtttctc
    14821 gcgcattacc catctggctg ctggggtaaa tacggatcac catattacct tttgacagct
    14881 gttttacttc tttcgccagc tcttcaaaac tctgatgtac aacatgactt ctttccaggt
    14941 tgtgtgctaa ctttaacgtc actttttctg cggcgtttac gccagtgcta accagagcca
    15001 gcaaggatgc gcagactaat gagcgggtaa atgacctcat tttcataaaa aaactccctg
    15061 ttgaagaatg gaaattggtt gatatctaat tgtgcttatc agtgctttgt gcgacaaatt
    15121 ggttaacctg tctgacaact tgtctatgtt tatcacaact tgaaataaag tcagaaatta
    15181 a
//
LOCUS       DAZKJR010000122        15159 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKJR010000122 DAZKJR010000000
VERSION     DAZKJR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.123"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(127..2406)
                     /gene="maeB"
                     /locus_tag="ACHOWS_003881"
     CDS             complement(127..2406)
                     /gene="maeB"
                     /locus_tag="ACHOWS_003881"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFQ0049387.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYRGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2695..3645
                     /gene="tal"
                     /locus_tag="ACHOWS_003882"
     CDS             2695..3645
                     /gene="tal"
                     /locus_tag="ACHOWS_003882"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFQ0049388.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3665..5668
                     /gene="tkt"
                     /locus_tag="ACHOWS_003883"
     CDS             3665..5668
                     /gene="tkt"
                     /locus_tag="ACHOWS_003883"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0049389.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKYAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIAYGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5771..6814)
                     /gene="ypfG"
                     /locus_tag="ACHOWS_003884"
     CDS             complement(5771..6814)
                     /gene="ypfG"
                     /locus_tag="ACHOWS_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFQ0049390.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6940..7515)
                     /gene="nudK"
                     /locus_tag="ACHOWS_003885"
     CDS             complement(6940..7515)
                     /gene="nudK"
                     /locus_tag="ACHOWS_003885"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFQ0049391.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7583..9562)
                     /gene="aegA"
                     /locus_tag="ACHOWS_003886"
     CDS             complement(7583..9562)
                     /gene="aegA"
                     /locus_tag="ACHOWS_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFQ0049392.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QVQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9768..11468
                     /gene="narQ"
                     /locus_tag="ACHOWS_003887"
     CDS             9768..11468
                     /gene="narQ"
                     /locus_tag="ACHOWS_003887"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFQ0049393.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11632..14745
                     /gene="acrD"
                     /locus_tag="ACHOWS_003888"
     CDS             11632..14745
                     /gene="acrD"
                     /locus_tag="ACHOWS_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFQ0049394.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAQLVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14844..14903)
                     /gene="ypfM"
                     /locus_tag="ACHOWS_003889"
     CDS             complement(14844..14903)
                     /gene="ypfM"
                     /locus_tag="ACHOWS_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFQ0049395.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3573 a         4007 c         3931 g         3648 t
ORIGIN      
        1 aatatcgaga tattttatat actgattttt ttattattcg taaactcagt aataagcttt
       61 actaatatat tgcagctaaa ctgcttaccc tgaatattca gggtaagcgt gagagttaaa
      121 aaaaaattac agcggttggg tttgcgcttc aaccacggcc agcgccacca tattgacgat
      181 acgacgtacc gatgcgatcg gcgttaacac gtgaaccggt ttcgccacac ccatcagcac
      241 cggaccgaca gtcacgcctt ccgagctgga aacgcgcagt aagttgtaac taatgcgggc
      301 agcttccatg ttcggcatca ccagaatatt agcggaacct ttcaaagggc tgtccggcat
      361 acggtcattc cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat
      421 cagttctggt gcacgttccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg
      481 gcagtcagaa gaaccaaagt tggagtgcga caacagagcc acacgcggct caataccaaa
      541 acgacgaaca gtttctgccg ccatcaaggt gatctccgcc agctcttctg catcaggctc
      601 atcattgacg taggtatcgg caataaaggt gttaccactc ggcaacagca gcgcgttcat
      661 tgcacctgcg gtgtgaacgc catcgcgata accaaagaca tttttcacca cgctaaagtg
      721 ttcatgataa tcgcccaccg taccgcaaat cattgcatcg gcttcgccac gctgaaccat
      781 gatcgcgccg atcactgtcg ggttactgat cagcgcacgc tgcgcctgct cctgagtgac
      841 accgcgacgc ttcatgatct ggaagtattc ggtccagtac tctttaaagc gcggatcgga
      901 ttcgttattg acgatctcaa aatcaacgcc cgccttgatc tgcaagccca gtttctgaat
      961 gcgcatttcg atcacgttcg gacgaccaat aaggatcggt ttcgccagtc ccagcgttac
     1021 cagttcctga gtggcatgca gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt
     1081 cggcgctttg cgagcctggg agaaaatcgg cttcataaac aggttagttt tgtaaacgaa
     1141 ctccgtcagc ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga
     1201 ctccatcgcc gctttcgcca ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg
     1261 ttttggaatg atgtattccg gaccaaagct cagatcctga tcgccatacg ctgaagccac
     1321 cacttcactc tgttccgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat
     1381 ctcttcgttg atggcggttg cgccaacgtc cagcgcgcca cggaagataa acgggaagca
     1441 aaggacgttg ttcacctggt tcgggtagtc ggaacggccg gtacagatga tggcatccgg
     1501 acgcacttct ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat
     1561 cattggcgca cgggccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca
     1621 gccgaggaaa atatccgcgc cttcaatcac atcatcgagg gtacgtttac cgtcatccac
     1681 caccgcgtat gcggctttgg tttccgccat gtttggctca cggccccgat agataacgcc
     1741 ttttgaatcg caaaccacga tgttatgttt ttgcagaccc agcgctacca gcaggttcat
     1801 acaggcgatt gctgcggcac ccgcgccaga aaccaccatc cgcacgtcgg agatgttttt
     1861 ctccaccacg cgcaaaccgt tgaggatggc ggcagtgctg ataattgccg tgccgtgctg
     1921 atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca
     1981 ttctggcgct ttaatgtctt cgaggttgat gccgccaaag gtcggttcga gcgcagcgac
     2041 gacttcaata aatttgtccg ggtcgagttc atcaacttct atgtcaaata catcaatccc
     2101 ggcgaatttc ttaaacagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc
     2161 aatgttgcct aaccccagca ccgccgtacc gttagagatc accgctacca ggttaccgcg
     2221 cgcggtatat ttgtaggctt ttaacgggtc tttttcgatt tcaagacaag gtgcggcaac
     2281 gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac
     2341 ctggattttc cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc
     2401 atccatttgg ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg caacagcccc
     2461 tgcccacaac gtggcgcaca ttattacctt gccggagtct acagactttg agcaagtcca
     2521 aactctcacc attaatataa tgttttggta ataatcctat aacactgatg ttacctgctt
     2581 aatccagcaa taccatgcct gtctgctatg cttttttgat gcgtttagcg aaatttctca
     2641 gaagtgtgaa ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac
     2701 gagttagacg gcatcaaaca gttcaccact gtcgtggcag acagcggcga tattgagtcc
     2761 attcgccatt atcatcccca ggatgccacc accaatcctt cgctgttact caaggctgcc
     2821 ggattatcac aatatgagca tttaatagac gatgctatcg cctggggtaa aaaaaatggc
     2881 aagacccagg agcaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct
     2941 gaaattctca aaatcgtacc cggtcgcgtg tcaacagaag ttgatgcacg cctctctttt
     3001 gataaagaaa agagtattga gaaggcgcgc catctggtgg acttgtatca gcaacaaggc
     3061 gttgagaaat cacgcattct gatcaagctg gcttcgacct gggaaggaat tcgcgcggca
     3121 gaagagctgg aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttgcacag
     3181 gcgcgggcct gtgcagaagc aggcgttttt ctgatttcgc cgtttgtcgg gcgtatttat
     3241 gactggtatc aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccaggcgtt
     3301 aaatcagtac gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg
     3361 ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga ccgactgact
     3421 atcgcaccga atttgctgaa ggagctacag gaaaaagttt cgccagtggt acgtaaatta
     3481 atcccacctt ctcagacgtt cccacgccca gctcccatga gcgaagcgga attccgttgg
     3541 gagcacaatc aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgcc
     3601 gttgatcaac gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg
     3661 ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca
     3721 aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct
     3781 gtggaacgat tttcttaaac ataatcctac cgacccaacc tggtatgatc gcgaccgctt
     3841 tattctttcc aacggtcacg cgtcgatgct gctctacagt ttgctgcatc tgaccggtta
     3901 cgacctgcca ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa cccctggtca
     3961 cccggaaatc ggctatacgc caggtgttga aaccaccacc ggtcctcttg gacaaggttt
     4021 ggcgaacgcc gtcgggctgg cgatagcaga gcgtacgctg gcggcgcagt ttaatcagcc
     4081 ggatcatgaa attgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga
     4141 aggtatctct cacgaagtct gttcgctggc gggcacgctg gggctgggca agctgattgg
     4201 tttttacgat cacaacggta tttccatcga cggtgaaacc gaaggctggt ttaccgacga
     4261 tacggcaaaa cgttttgaag cctatcactg gcatgtgatc cacgagatcg acggtcacga
     4321 tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc gtgaaagata agccgtcgct
     4381 gattatctgc cgtacggtga ttggctttgg atcgccgaat aaagcaggta aagaagaggc
     4441 gcacggcgca ccgctgggtg aagaagaagt ggcattggca cggcaaaaac tgggctggca
     4501 ccatccgcca tttgagatcc ctaaagagat ttatcacgcc tgggatgccc gcgaaaaagg
     4561 cgaaaaagcg cagcagagct ggaatgagaa gtatgcagcc tacaaaaagg cccatccgca
     4621 actggcagaa gagtttaccc gtcggatgag cggtggttta ccgaaggact gggagaaaac
     4681 gactcagaaa tatatcaatg aattacaggc aaatccggcg aaaatcgcta cccgtaaggc
     4741 ttcgcaaaat acgcttaacg cttacgggcc aatgcttcct gaattgctcg gcggttcggc
     4801 ggatctggct cccagcaacc tcaccatctg gaaaggttct gtgtcgctga aggaggatcc
     4861 ggcgggcaac tacattcact acggggtgcg ggaatttggc atgaccgcta tcgcctatgg
     4921 cattgcgcac cacggcggct tcgtgccgta taccgcgacg ttcctgatgt ttgttgaata
     4981 cgctcgtaac gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac
     5041 ccacgactca attggcttag gggaagatgg tccgacccac caggccgttg agcaactggc
     5101 cagtctgcgc ttaacgccaa atttcagcac ctggcgaccg tgcgatcagg tggaagcggc
     5161 ggttggctgg aagctggcgg ttgagcgcca caacggaccg acggcactga tcctctccag
     5221 gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg
     5281 ttatgtactg aaagacagcg gcggtaagcc agatattatt ttgattgcca ccggttcaga
     5341 gatggaaatc accctgcaag cggcagagaa attagcagga gaaggtcgca atgtacgcgt
     5401 agtttcgctg ccctcgaccg atattttcga cgcccaggat gaggaatatc gggagtcggt
     5461 gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca ggcatcgccg attactggta
     5521 caaatatgtt ggtctgaaag gggcaattgt cgggatgacc ggctatgggg aatctgctcc
     5581 ggcagataag ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca
     5641 taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg gatgctatat gttcccctca
     5701 ccctaaccct ctccccagag gggcgagggg acggtccgtg cgctttttag gggcgcatct
     5761 gggattttgt ttaacgggtg atccacaacg tgggccaggc gtctggccca tgccagttat
     5821 cgcaggtggg ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc
     5881 gcgcctgaat gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcacgcg
     5941 atttttcatc gaatgttgcg ttcatcagtt ccagttcatt cgtctcctga ccgctgttga
     6001 acggcaaacg taaccgaacc gggcgcgagg ccagtggctt tttacgcgac acaatccatg
     6061 ccaaatcaat ggtgttatag gcccctgcct cacagctaat catcatcagc gctttatcat
     6121 cggtcagcgc agtgacattc acctctcgac gcaatggatc aagcgagcag cgcagaccat
     6181 tcatccgcca gtttccataa tccagcaaat cgttgcgttc ttcgagtgag agtggcgtcg
     6241 gcgttgggtt aaccaccgcg acctctttca gcgcaggcgc gggcggtacg ctgagcggcg
     6301 gttcatcccc tttctttatc cacgcggttt cactgccaac gcgcttttgc tgagcatcta
     6361 taaacaacaa cgccgctttt aagccactca gagaaatagt ctgattgccg tcgcgtaagg
     6421 tgatcgccct cccttcctga atcatctgca aaaacgcggt gatggttgcc gtatcatcgg
     6481 ttactaataa ccatggtgaa atccgccact tgtcaccact taacgccaac ggctcaccat
     6541 ctaacagcag ccgtggcgct atctcccctt cagacgcatc cggcgacttc aacccgccgc
     6601 gctcaatacg taaaacggca tcggtatgcg ccccggcgct gcggctcagg gtcatcacca
     6661 gtccgttatg atcgcccgta ttacgcgcca cgcagaagtt ttggttatta caggtgacct
     6721 gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag
     6781 cgaaaaaaaa gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa
     6841 caaacagcac aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttatcgga
     6901 ttgagccaat aataaacaac gaaatattat tgttattttt cagtccatta aatgtgacat
     6961 ttgcaaatag ttaagcaata acaccgtctt accgtcacgt atctcgccag ttttgatcat
     7021 ctccagcgcc ttgttgaacg gcagctcgag cacttcaata tcttcatctt cgacaccacc
     7081 gccggcgtta gcgcgctggc tgtcactgta ttcggcgata aagaagtgga ttagctcagt
     7141 cacaccgcct ggcgacatat acagttcaaa taatttgcgc acttcgccaa cttcataacc
     7201 cgtctcttca atcgcttctt tacgaataca cacttccggt tcgtcgttat ccagcagccc
     7261 ggcgcaggtt tcaatcagtt gcccgctttc attgccatta acccaggtag cgacgcggaa
     7321 ctggcgaatc agaaccacgg tctttttctt cgcgttgtac aggaggatcg ttgcaccatt
     7381 accgcgatcg taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc
     7441 gtaagtaatg ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt
     7501 gatttgttgc gtcataccga ctccacagcg cgaaatgaac aattatctta cgctgtgaag
     7561 ccgggtttgt ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa
     7621 taatcccctg tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac
     7681 gcacggcgtc accaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt
     7741 ccacatccgc gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg
     7801 gcatcccgtg cggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt
     7861 cactgccttc caccggcact ggacgccgac gcccctgggc atccggctcg ccaagacgcg
     7921 tgcgcaggaa acgaatcccg cagacgtgac cttgttcatt cagttcaagc gccaccggct
     7981 ggacgttaaa ttcgaagttg gccccctctt cacgggcgtt cttcacctct ttcttcgagc
     8041 ctggcatgtt agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca
     8101 gcgcagtacg cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc
     8161 cggcagtatt gataaacggc tcttccggta gctcttcgag tcccatcacc tgtttagtat
     8221 tggcgatgag gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg
     8281 ctttcatgga gcggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg
     8341 aattcaaaga gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc
     8401 tgaagatttc ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga
     8461 aagtgagcaa gccaccgatt tctggatggc gatcgtacac cgtcacctct acgccattgc
     8521 gggtcaaaac atccgcacag gccagccctg ccggacccgc accgataatc gccacccgct
     8581 tgtccacttt ggtgacatgg cttaagtcag gacgccaacc tttcgccaac gcctgatctg
     8641 aaatgtagcg ttcaatgtta ccgatagtta ctgcgccgtg ctcatcgcga atagtacagg
     8701 caccttcaca caaacggtct tgcgggcaaa cgcgtccggt aatttccggc agggtgttgg
     8761 tctggtgaga aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact
     8821 gcggtatatg gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgcacttaa
     8881 ggcagcgcga ggcttcccgt tgtacctgat cggcacggaa tggcagataa atttcatcaa
     8941 aaccggtttt gcgcgcttca atcgccagtt tatctggctc gccacgcgcg ggcgttgcct
     9001 gcatttgctc gactttactc attaccggca tttcttgcgc cgcggtactg gcatgccacg
     9061 gttgatgttc ctgacgtgcg gtacgcaagc ggcgggattt cgccatgcct gagagtgcgg
     9121 cgtcagtgac cagttgcagc gcgtccgctg ggcaattctc gacacaagca ggaccgtttt
     9181 cgcgccctgc acaaaggtcg catttatgcg ccgtggcttt tacttttcct gccgcgacgg
     9241 gggtcaggac gatttgcatc gtaccaaatg gacaggccac cacgcaggat ttacagccaa
     9301 tacacttttg ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc
     9361 agctacgggc gcagggcgca tcttcacaat ggtgacaggt cacggcacta cgttgctgct
     9421 gatgtttgat aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat
     9481 cattgtgagc catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt
     9541 tggccataat aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt
     9601 atatgagtgt tgttattacc cgacttacag gggaatcggc aatgttcatg tgcccagaat
     9661 aagtaactat ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgc
     9721 gtttcagcga attggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac
     9781 gacccgtctc ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt
     9841 ccacgggtat cgctctgcta acgctggcga gcagtttacg cgacgctgag gctatcaata
     9901 ttgccggatc gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc
     9961 cacaactcaa tgcacatcgc cagttatttc agcaggcact gcattcaccg gtattaacca
    10021 atctcaacgt ctggtatgtg ccagaggcag taaaaacccg ctatgcgcat ctgaatgcca
    10081 actggctgga gatgaataat cggctcagca agggcgactt gccgtggtat caggccaata
    10141 ttaataatta tgttaatcag atagacctgt tcgtgctggc tttacagcac tacgccgaac
    10201 gcaaaatgct gctggtggtg gcgatctccc tggctggcgg catcggtatt ttcacgctgg
    10261 tcttttttac tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta
    10321 ccgccagtca gcgtattgaa cacgggcagt tcgactcgcc gccgctggat accagcctgc
    10381 cgaatgagct tggcctgctt gcaaaaacct ttaaccagat gtcgagcgag ctgcataaat
    10441 tgtaccgttc gctggaggcg tcagtagaag aaaagacccg cgatctccac gaggccaagc
    10501 gtcgtctgga ggtgttgtat cagtgttcgc aggcgctaaa caccagccag attgatgtgc
    10561 attgtttccg ccatattttg cagattgttc gcgacaatga agcggctgaa tatctggagt
    10621 taaatgtcgg tgacaactgg cggattagcg aagggcagcc aaacccggaa ttgccgatgc
    10681 agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg caaaatagtc
    10741 acgtttcatc atcagaaccg ctgcttaaca gtgtttcgtc gatgctggga cgcggtttgt
    10801 actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa cgtgcgacca
    10861 tcgcccgcga attgcacgac tcgctggctc aggtactttc ttacttacgt atccagttga
    10921 cgttactgaa gcgttcgata ccggaagata acgccaccgc acaaagtatc atggccgatt
    10981 tctcccaggc gctgaatgat gcttatcggc agttacgcga gctgttaact actttccgcc
    11041 tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat acgttacaaa
    11101 atcaaaccag cgccaaactg accctcgact gccgtctgcc aaccctggcg ctggatgcgc
    11161 aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg atgaagcacg
    11221 ccaacgccag cgaaatcgcc gttagctgcg tcaccgcgcc ggatggcaat cacacagtct
    11281 atattcgcga caacgggatt ggcatcggtg aaccgaaaga acccgaaggc cattatggtc
    11341 tgaatatcat gcgcgaacgc gcggaaagat tgggtggaac gctgactttt tcacaacctt
    11401 ccggcggcgg cacgttagtg agtattagct ttcgctctgc ggagggtgag gaaagtcagc
    11461 tgatgtaatg cctcttattg accaaagaat agtggcactt aaggttcagt ataaaagggc
    11521 atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag
    11581 tgaagcaagt caagcctaca acgatacgca gaaacacgag gtcctctttt aatggcgaat
    11641 ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggt
    11701 accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg
    11761 cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag
    11821 gttatcgagc aaaatatgac cggcctcgat aatctcatgt atatgtcgtc tcagagcagt
    11881 ggcaccggcc aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc
    11941 gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa
    12001 aatcagggcg tgacggtgcg taaaaccggc gataccaata ttctgaccat tgccttcgtc
    12061 tctaccgatg gttcgatgga taaacaggat attgccgatt acgttgccag taatattcag
    12121 gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc
    12181 atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc
    12241 actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacgcct
    12301 tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca
    12361 gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gttcagaggt aaggctgggc
    12421 gatgtcgcca ccgtcgaaat gggggcggaa aaatacgatt atcttagccg cttcaatggt
    12481 aagccagcct ccgggctggg ggtaaaactg gcctccggcg cgaacgaaat ggcgactgcg
    12541 cagctggtgc tcaatcgtct cgacgagctg gcgcagtatt ttccacacgg actggaatac
    12601 aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg
    12661 ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc
    12721 gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt
    12781 tatgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt
    12841 ctgctggtgg atgatgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa
    12901 ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc
    12961 gggattgcga tggtgctttc ggcggtattt gtgccaatgg ccttcttcgg cggcaccacc
    13021 ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg
    13081 gtagcgatga tcctcactcc ggctctgtgt gccacgctac ttaagccact gaaaaaaggt
    13141 gagcatcacg ggcaaaaagg cttttttgcc tggtttaacc agatgtttaa ccgcaacgcc
    13201 gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt
    13261 tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttcttg
    13321 ccacttgaag accgtggcat gtttactacc tcggtacagt tgcccagcgg ttcaacccaa
    13381 caacagaccc tgaaagtcgt tgagcaaatc gagaaatact acttcaccca tgaaaaagac
    13441 aacatcatgt cggtgtttgc cactgttggt tctggccctg ggggtaacgg acaaaacgtg
    13501 gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg
    13561 tttgccatta tcgagcgtgc aacgaaggcg tttaacaaga ttaaagaagc tcgcgttatc
    13621 gccagcagcc cgccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg
    13681 caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaacca gttgctggcg
    13741 ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga cgacagtccg
    13801 cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttgc tatcgacgat
    13861 attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc
    13921 ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac
    13981 atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg
    14041 acctcacgct gggagacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggta
    14101 gagattgtag gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa
    14161 tcgttagtga agcagttgcc aaacggcttt ggtctggagt ggacggcgat gtcgtatcag
    14221 gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc
    14281 ctgtgtcttg cggcgttgta tgaaagctgg tcggtgccgt tctcggtaat gctggtcgtg
    14341 ccgctggggg ttatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt
    14401 tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc
    14461 gtcgagtttg ctaacgagat gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac
    14521 gcctgccgtc agcgtttacg cccgattctg atgacttcgc tggcatttat cttcggcgta
    14581 ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc
    14641 gtaatgggcg ggatgatttc ggccactatt ctggctattt acttcgtgcc gctgttcttt
    14701 gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg
    14761 acatgctaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg
    14821 ctgtttgtac tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag
    14881 ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa
    14941 caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa
    15001 accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt
    15061 cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca gacagataac
    15121 aagatgaata ttcttaatgt ttacgttaaa aatgtttaa
//
LOCUS       DAZKJR010000123        15153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKJR010000123 DAZKJR010000000
VERSION     DAZKJR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.124"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..936
                     /gene="yfiE"
                     /locus_tag="ACHOWS_003890"
     CDS             55..936
                     /gene="yfiE"
                     /locus_tag="ACHOWS_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0049396.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(969..2303)
                     /gene="srmB"
                     /locus_tag="ACHOWS_003891"
     CDS             complement(969..2303)
                     /gene="srmB"
                     /locus_tag="ACHOWS_003891"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFQ0049397.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            2435..3172
                     /gene="trmN"
                     /locus_tag="ACHOWS_003892"
     CDS             2435..3172
                     /gene="trmN"
                     /locus_tag="ACHOWS_003892"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFQ0049398.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(3157..4779)
                     /gene="nadB"
                     /locus_tag="ACHOWS_003893"
     CDS             complement(3157..4779)
                     /gene="nadB"
                     /locus_tag="ACHOWS_003893"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFQ0049399.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDDRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            5035..5190
                     /gene="rseD"
                     /locus_tag="ACHOWS_003894"
     CDS             5035..5190
                     /gene="rseD"
                     /locus_tag="ACHOWS_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFQ0049400.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            5187..5762
                     /gene="rpoE"
                     /locus_tag="ACHOWS_003895"
     CDS             5187..5762
                     /gene="rpoE"
                     /locus_tag="ACHOWS_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFQ0049401.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            5795..6445
                     /gene="rseA"
                     /locus_tag="ACHOWS_003896"
     CDS             5795..6445
                     /gene="rseA"
                     /locus_tag="ACHOWS_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFQ0049402.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            6445..7401
                     /gene="rseB"
                     /locus_tag="ACHOWS_003897"
     CDS             6445..7401
                     /gene="rseB"
                     /locus_tag="ACHOWS_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFQ0049403.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            7398..7877
                     /gene="rseC"
                     /locus_tag="ACHOWS_003898"
     CDS             7398..7877
                     /gene="rseC"
                     /locus_tag="ACHOWS_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFQ0049404.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            8075..9874
                     /gene="lepA"
                     /locus_tag="ACHOWS_003899"
     CDS             8075..9874
                     /gene="lepA"
                     /locus_tag="ACHOWS_003899"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0049405.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            9890..10864
                     /gene="lepB"
                     /locus_tag="ACHOWS_003900"
     CDS             9890..10864
                     /gene="lepB"
                     /locus_tag="ACHOWS_003900"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0049406.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            11136..11816
                     /gene="rnc"
                     /locus_tag="ACHOWS_003901"
     CDS             11136..11816
                     /gene="rnc"
                     /locus_tag="ACHOWS_003901"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0049407.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            11813..12718
                     /gene="era"
                     /locus_tag="ACHOWS_003902"
     CDS             11813..12718
                     /gene="era"
                     /locus_tag="ACHOWS_003902"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0049408.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            12730..13458
                     /gene="recO"
                     /locus_tag="ACHOWS_003903"
     CDS             12730..13458
                     /gene="recO"
                     /locus_tag="ACHOWS_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0049409.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGESVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            13470..14201
                     /gene="pdxJ"
                     /locus_tag="ACHOWS_003904"
     CDS             13470..14201
                     /gene="pdxJ"
                     /locus_tag="ACHOWS_003904"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFQ0049410.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIPVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            14201..14581
                     /gene="acpS"
                     /locus_tag="ACHOWS_003905"
     CDS             14201..14581
                     /gene="acpS"
                     /locus_tag="ACHOWS_003905"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0049411.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            15003..15083
                     /gene="shoB"
                     /locus_tag="ACHOWS_003906"
     CDS             15003..15083
                     /gene="shoB"
                     /locus_tag="ACHOWS_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFQ0049412.1"
                     /translation="MTDCRYLIKRIIKIIIAVLQLILLFL"
BASE COUNT         3692 a         3729 c         4056 g         3676 t
ORIGIN      
        1 aaaaaatgaa tgtttttaca ttctaaattc gatattttcg ataggtttgg ggttatggat
       61 ctgcgccgtt ttattacact taaaaccgtg gtggaagagg gttccttttt gcgagcttcg
      121 caaaaattgt gctgcacaca atcgacggtg acttttcata ttcagcagct tgagcaggag
      181 ttctcagtcc agttatttga gaaaattggt cgacgaatgt gccttacccg cgaaggaaaa
      241 aagctgctgc cgcacattta tgaactcacc agagtgatgg atacccttcg tgaggctgcc
      301 aaaaaagagt cggatccgga cggggaacta cgcgttattt caggcgaaac gttgctctct
      361 tatcgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg ctccaaaagt gcgtttatcg
      421 ttgcagtcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga ggccgatgtc
      481 ggggtttttt atcgcgtagg gaatgatgat gctttgaacc gacgagagtt gggtgaacaa
      541 ccgctggtgc tggtggcttc accgcaaatt gcagatgtcg attttactga gccgggaaga
      601 cataacgcct gtagctttat tatcaacgaa ccacaatgtg tcttccggca gatatttgag
      661 agcacgctgc gccagcgacg gatcacggtg gaaaacacca ttgagcttat aagtatcgaa
      721 agcatcaagc gttgtgtcgc ggcaaatatc ggggtcagtt acctgccgcg ctttacagtg
      781 gcaaatgaac ttgcgagtgg cgaattaatc gaattgcctt ttggcgaaca gtcgcagacc
      841 attacggcca tgtgcgcaca ccatgctgga aaagcggtta gcccggcgat gcacactttt
      901 attcagtgtg ttgaagagtg ttttttgccg ggataaaaac aatgccgggc ttaaacccgg
      961 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
     1021 ttaccaatat ttttagtgtc gcgatggcgt tttttcaccc gcggcttctc tttctcttta
     1081 gcttttttct tctcggcacg tttagccaac actttcttcg atggcttgcc ggtctgcttc
     1141 tcgcttggcg cacgcgtttt cggacgtaac tcatcaatta cgcgagcttt aattggctct
     1201 tcaatataac ggccaacttt gcccagcagc agatggtcat gggcttccac cagcgaaatc
     1261 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
     1321 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
     1381 gcaacatcgg tagcaaccag tacgtttacg cggccttcgg tcaagcgttt aatcgcttcg
     1441 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc tgcttcgcgc
     1501 agccagtttg ccagctcatg cacacgctca cgcttacgca caaacacaat tgagcgggtc
     1561 gcttccggct gttttaacag atgcaccagc aacgcggttt tatgctcaag atcatcggcg
     1621 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
     1681 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagagttgcc
     1741 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctgg
     1801 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
     1861 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
     1921 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
     1981 gcaatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
     2041 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
     2101 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
     2161 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
     2221 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
     2281 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
     2341 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
     2401 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
     2461 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgttgtg cgatgaaagt
     2521 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtaa aacgttgcct
     2581 tgatatcggc gcgggtagcg ggttgctggc attaatgctg gcgcagcgaa ccgatgacag
     2641 cgtaatgatt gatgcagttg aactggaaag tgaagctgcc gcgcaggcgc aggaaaatat
     2701 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggat
     2761 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
     2821 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
     2881 atcgttgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
     2941 gccagagcag attggtaatg gttttactga actggcatta agtatgggct ggcatttacg
     3001 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
     3061 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
     3121 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
     3181 gggggaaagg atcgacggac cggaatgggt gagcagttcc ggataatcca gcgtgaaatg
     3241 caacccccga ctctctttac gcatcattgc acagcgaaca atcaactcgg caacctgtac
     3301 cagattacgc agctccagca aattatttga gacgcggaaa tgggcgtaat attcgtctat
     3361 ttcttgctgg agcatggtta tccgccgcag ggcgcgttcc aggcgcttcg ttgtgcgcac
     3421 aatgccgacg taatcccaca taaacagacg tagctcgtgc cagttatgtt gaatcaccac
     3481 ccgttcatca gggttctcaa cgcggctttc atcccacggc ggtaacgtac tgacgccgtg
     3541 ggcataaggc atacatctgg tgatatcttc tgccgccgac cagccataga ccagacactc
     3601 cagcaaggag ttagaggcca tgcggttagc gccgtgtaag ccggtataac tcacttcgcc
     3661 aatggcatac aaaccctcga cgtccgtacg cccatgatca tccaccatca caccaccgca
     3721 ggtatagtgt gcagcaggca caatcggtac tggttcttgt gtgagatcaa tccccagccc
     3781 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
     3841 gatgtcgagg aacatacaat ccgcgccgag gcgtttcatt tcatggtcaa tagcgcgggc
     3901 gacaatatcg cgcggggcca gttcgccgcg atcatcaaaa tcgggcataa aacgcgtacc
     3961 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tctgttaaca ggaaattgcg
     4021 tgcctgtggg tgatacagtg cggtagggtg gaactgatta aattcgagat tggcaacccg
     4081 gcagcctgcg cgccacgcca tagcaatgcc atcgccagaa gaaatatccg gattggtggt
     4141 gtactgataa accttcgacg caccgccggt tgccagtacc actgcttttg cgtggcaggt
     4201 ttccaccgtt tctttatttc ggttccatac ccacgcgcca acaacccgtc gcgtgcccgg
     4261 caggccaatt ttgtcagaaa caatcagatc aaccgcgttg ctgcgctcca gcacgcgaat
     4321 attcggatgg ttcagcgcct tgctcaccag cgtggtttct acttctctac cggtggcgtc
     4381 ggcggcatga agaattcgac ggtgactatg tccaccttca cgggtcagat ggtagctttc
     4441 tgcaccattc ggttgaatgt gggtatcaaa caacaccccc tggtcgatta gccattgcac
     4501 acaggatcgt gcattgctgg cgacaaattc aactgcatga cgatcgcaaa taccagcccc
     4561 ggcaatcaat gtgtcttcca catgcgagtc aatgctgtca gtctcatcaa acacggcggc
     4621 tataccgccc tgggcataaa atgttgaacc ttccgttacc gggcctttac ttagaacgat
     4681 gacctgatgc tggtcagcca ggcgtagcgc cagtgaaagt ccagctgcgc cgctaccgat
     4741 aatcaacacg tcacatgaat gttcagggag agtattcatt ttctttgttt aatttactaa
     4801 acatggtttg gtcagcatag catcatgttg tgcgattaaa cacctgctat tttaatattt
     4861 gttacagtga ctaaacacgc tgactcaggg cggcgagaaa aagagaagtt actggctggt
     4921 ggaggattag gtggtgaaat aaaaaggccg ttgggttact cttcaggcag ttaaatgggc
     4981 atttctacac agataatgcg atgttcagat tctgtagact tataatgata gataatgatc
     5041 cgtctacagc atgacaaaca aaaacagatg cgttacggaa ctttacaaaa acgagacact
     5101 ctaacccttt gcttgctcaa attgcagcta atggagtggc gtttcgatag cgcgtggaaa
     5161 tttggtttgg ggagacttta cctcggatga gcgagcagtt aacggaccag gtcctggttg
     5221 aacgggtcca gaagggagat cagaaagcct ttaacttact ggtagtgcgc tatcagcata
     5281 aagtggcgag tctggtttcc cgctatgtgc cgtcgggtga tgttcccgat gtggtacaag
     5341 aagcttttat taaagcctat cgtgcgctgg attcgttccg gggagatagc gctttttata
     5401 catggctgta tcggattgct gtaaatacag cgaaaaatta cctggttgct caggggcgtc
     5461 gtccaccttc cagtgatgtg gatgccattg aagctgaaaa cttcgaaagt ggcggcgcgt
     5521 tgaaagaaat ttcgaaccct gagaacttaa tgttgtcaga agaactgaga cagatagttt
     5581 tccgaactat tgagtccctc ccggaagatt tacgcatggc aataaccttg cgggagctgg
     5641 atggcctgag ctatgaagag atagccgcta tcatggattg tccggtaggt acggtgcgtt
     5701 cacgtatctt ccgagcgagg gaagctattg ataacaaagt tcaaccgctt atcaggcgtt
     5761 gacgatagcg ggatactgga taagggtatt aggcatgcag aaagaacaac tttccgcttt
     5821 aatggatggc gaaacgctgg atagtgagct gcttaacgaa ctggctcata acccagaaat
     5881 gcagaaaacc tgggaaagct atcacttaat ccgtgactca atgcggggtg atactcccga
     5941 ggtgctccat ttcgatatct cttcacgcgt gatggccgcc attgaagaag agccagtacg
     6001 tcaaccggcg acattgatcc cggaagccca gcctgcgccg catcaatggc agaaaatgcc
     6061 attctggcag aaagtacgtc cgtgggcggc acagcttacc caaatgggcg tagccgcatg
     6121 cgtatcgctt gcagttatcg ttggcgtcca gcactataat ggacaatctg aaacgtccca
     6181 gcagcccgaa acgccggtat ttaatacact gccgatgatg ggtaaagcca gcccggtaag
     6241 cctgggagta ccttctgaag cgaccgcaaa caatggtcaa cagcagcagg tacaggagca
     6301 gcgtcgtcgc attaatgcaa tgttgcagga ttacgaactg caacgccgac tccactctga
     6361 acagcttcag tttgagcagg cgcaaactca gcaagccgct gtacaggtgc caggaattca
     6421 aactttagga acgcaatcgc agtaatgaag caactttggt ttgccatgtc attagtgaca
     6481 ggtagcctgt tattctctgc taacgcctcg gccactcccg cgtccggggc gttattacag
     6541 cagatgaacc tggccagtca gtcactgaat tacgagctgt cattcatcag catcaataaa
     6601 cagggtgttg agtctctgcg ttatcgacat gcacgcctcg ataaccgtcc tcttgcacaa
     6661 ttgttgcaaa tggatggccc gcgccgggaa gtggtacagc gcggcaatga aatcagctat
     6721 tttgaaccgg gacttgaacc gttcacgctt aatggcgatt acattgttga ttctctacca
     6781 tcgctcatct ataccgattt caaacgcctt tctccttact acgactttat ctccgtcggg
     6841 cgcacgcgta ttgctgatcg tctttgcgaa gtcattcgtg tggttgcccg agatggtaca
     6901 cgctacagct acatcgtgtg gatggacacc gaatcgaaat taccgatgcg ggttgatctc
     6961 cttgatcgcg atggtgaaac gctggaacaa tttcgcgtga ttgcttttaa tgtcaatcag
     7021 gatatcagca gcagtatgca gacgctggcg aaggcaaatt tgccgccgtt gctttctgtt
     7081 cctgtaggtg aaaaagctaa attcagctgg acgccaacct ggttgccaca gggttttagc
     7141 gaagtttcca gtagtcgacg tccgctaccg acgatggaca acatgcctat cgaatcacgt
     7201 ctctattccg acggattatt cagcttctcg gtaaacgtta accgcgctac gccatcgagc
     7261 accgatcaga tgttgcgcac cgggcgcaga accgtcagta caagcgtacg tgataacgcc
     7321 gaaatcacca ttgtcggtga actgccgccg caaacggcga aacgcattgc cgagaatatt
     7381 aagttcgggg cagcgcaatg atcaaagagt gggctaccgt cgtctcctgg gaaaacgggc
     7441 aggcgctggt tagttgtgat gttaaagcct catgcagcag ctgcgcttca cgtgccggtt
     7501 gcggtagccg cgtgttgaat aaacttggcc cgcaaaccac gcataccatt gtcgtaccct
     7561 gtgatgagcc gttagtgccg gggcaaaaag tagaattagg aatcgccgaa ggcagcctgc
     7621 ttagctccgc attactggtt tatatgtcgc cgttggtggg actattcctc atcgcttcac
     7681 tatttcagct actctttgct tcagatattg cagcattatg cggtgcgatt ctcggtggcg
     7741 ttggtggatt cctgattgca cgtggctgct ctcgaaagtt tgctgcccgg tctgaatggc
     7801 aaccgatcat cttaagcgtg gccttgccgc ctggccttgt gcgatttgaa acatcttcgg
     7861 aagacgcgag ccagtgattt cactggcttt gttgcatttt gcataacaaa tgcgaggacg
     7921 tttccagata ccacaatcca tacggtatga acatttcctc gcctcaatgt tgtagtgtag
     7981 aatgcggcgt ttctattaat acagacgtta agctcagaac agcgacttct aaaagcctgg
     8041 ttaaccgggc attaaggcac aataatcata ctttatgaag aatatacgta acttttcgat
     8101 catagctcac attgaccacg gtaaatcgac gctgtctgac cgtattatcc agatctgcgg
     8161 tggcctgtct gaccgtgaaa tggaggcgca ggttctcgat tccatggatc ttgagcgtga
     8221 gcgtggcatt accatcaaag cgcaaagcgt gacgctggac tacaaagcgt ctgacggcga
     8281 aacctatcag cttaacttta tcgacacccc gggccacgta gacttctcct atgaagtttc
     8341 tcgttcgctg gctgcctgtg aaggtgcatt gctggtggtc gacgccgggc agggcgtaga
     8401 agcgcaaacc ctggcaaact gctacaccgc catggaaatg gatctcgaag tggtgccggt
     8461 actgaacaag attgacctgc cggcagccga tcctgaacgc gtggcggaag aaattgaaga
     8521 tatcgtcggc atcgacgcca ccgacgcggt gcgctgttca gcgaaaaccg gcgttggcgt
     8581 acaggacgtt ctcgaacgtc tggtgcgcga cattccgccg ccggaaggcg atccggaagg
     8641 cccgttgcag gcactcatta tcgactcctg gttcgacaac tacctgggcg ttgtttcact
     8701 tatccgtatt aaaaacggca ccctgcgtaa gggcgacaaa gtgaaagtca tgagtaccgg
     8761 gcagacctat aacgccgacc gtctgggcat cttcacgccg aagcaggttg accgtactga
     8821 actgaaatgc ggcgaagtag gctggctggt atgtgcgatt aaagatatcc acggcgctcc
     8881 agtaggcgat accttaacgc tggcgcgtaa tccggcagaa aaggcgctgc ctggctttaa
     8941 gaaagtcaaa ccgcaggtat acgccggtct gttcccggta agttccgacg actatgaagc
     9001 cttccgtgac gcgctgggca aactcagcct gaacgatgct tcactgttct atgagccgga
     9061 aagctccagc gcgctgggct ttggtttccg ctgcggcttc ctcggcctgc tgcacatgga
     9121 gatcatccag gaacgtctgg aacgtgaata cgatctggat ctgatcacca ctgcgccgac
     9181 cgtagtgtat gaagttgaaa ccacgtcaag agaagttatc tacgtcgaca gcccatccaa
     9241 gctgccagcg gtaaataaca tttacgaact gcgcgagccg attgcagagt gtcacatgct
     9301 gctgccgcag gcatatctcg gcaacgttat tacgctgtgc gtagaaaaac gcggcgtgca
     9361 gaccaatatg gtttaccacg gtaatcaggt ggcgctgacg tacgagatcc cgatggcgga
     9421 agtggtgctc gacttcttcg atcgcctgaa atctacctcg cgtggttatg cgtctctgga
     9481 ttacaacttc aaacgcttcc aggcgtccga catggtacgt gtagacgtat taatcaacgg
     9541 tgaacgtgtt gatgcgctgg cgttgatcac ccaccgtgat aattcgcaaa accgcggtcg
     9601 cgagctggtg gagaagatga aagatctgat cccacgccag cagtttgata tcgccattca
     9661 ggcagcgatt ggtacgcaca tcattgcgcg ctccaccgtg aaacagctgc gtaaaaacgt
     9721 actggctaaa tgttatggcg gcgatatcag ccgtaagaaa aagctgctgc agaagcagaa
     9781 agaaggtaag aaacgcatga agcagatcgg taacgtcgag ctgccgcagg aagcgttcct
     9841 cgccattctg cacgtcggca aagacaacaa ataaccctta ggagttggca tggcgaatat
     9901 gtttgccctg attctggtga ttgccacact ggtgacgggc attttatggt gcgtggataa
     9961 attctttttc gcacctaaac ggcgggaacg tcaggcagcg gcgcaggcgg ctgccggtga
    10021 ctcactggat aaagcaacgc tgaagaaagt tgcgccgaag cctggctggc tggaaaccgg
    10081 tgcttctgtt tttccggtgc tggctatcgt attgattgtg cgttcgttta tttatgaacc
    10141 gttccagatc ccgtcaggtt cgatgatgcc gactctgtta atcggtgatt ttattctggt
    10201 agagaagttt gcttatggca ttaaagatcc tatctaccag aaaacgctga tcgaaaccgg
    10261 tcatccgaaa cgcggcgata tcgtggtctt taaatatccg gaagatccaa agcttgatta
    10321 catcaagcgc gcggtgggtt tgccgggcga taaagtcact tatgatccgg tctcaaaaga
    10381 gctgacgatt caaccgggat gcagttccgg ccaggcgtgc gaaaacgcgc taccggtcac
    10441 ttactccaac gtggaaccga gcgatttcgt tcagaccttc tcacgccgta atggtgggga
    10501 agcgaccagc ggattctttg aagtgccgaa aaacgaaacc aaagaaaatg gaattcgtct
    10561 ttccgagcgt aaagagacat tgggtgatgt gacgcaccgc attctgacag tgccgattgc
    10621 gcaggaccag gtggggatgt attaccagca gccagggcaa caactggcaa cctggattgt
    10681 tccgccggga caatacttca tgatgggcga caaccgcgac aacagcgcgg acagccgtta
    10741 ctggggcttt gtgcctgaag cgaatctggt cggtcgggca acggctatct ggatgagctt
    10801 cgataagcaa gaaggcgaat ggccgactgg tgtgcgctta agtcgcattg gcggtatcca
    10861 ttaatagcca tcttcgttca cgttgtcgcc gttatggcga caacgtgaat tatttatgag
    10921 ataaatctcc cgtggctaac gacatccccc gtcgttgtgt atagaatatt cccccgaagt
    10981 ttaaggttgg cacctccagg ttgccacggc acacgaaaca gcgttggtcc ccatataccg
    11041 gtaaactgaa actgcagtga agcagttagc cgagccatgt atatcaggtc tgtttcgtgt
    11101 gctgaattgt tgacgcattt atttattggt atcgcatgaa ccccatcgta attaatcggc
    11161 ttcaacggaa gctgggctac acttttaatc atcaggaact gttgcagcag gcattaactc
    11221 atcgtagtgc cagcagtaaa cataacgagc gtttagaatt tttaggtgac tctattctga
    11281 gctacgttat cgccaatgcg ctttatcacc gtttccctcg tgtggatgaa ggcgatatga
    11341 gccggatgcg cgccacgctg gtccgtggta atacgctggc tgaactggcg cgcgaatttg
    11401 agttaggtga gtgcttacgt ttagggccag gtgaacttaa aagcggtgga tttcgtcgtg
    11461 agtcaattct cgctgacacc gtcgaggcat taattggtgg cgtattcctc gacagtgata
    11521 ttcaaaccgt cgagaaatta atcctcaact ggtatcaaac tcgtctggac gaaattagcc
    11581 caggcgataa acaaaaagat ccgaaaacgc gtctgcagga atatttgcag ggtcgccatc
    11641 tgccgctgcc aacttatctg gtagtccagg tacgtggcga agcgcacgat caggaattta
    11701 ctatccactg ccaggtcagc ggcctgagtg aaccggtggt tggcacaggt tcaagccgtc
    11761 gtaaggctga gcaggctgcc gccgaacagg cgttgaaaaa actggagctg gaatgagcat
    11821 cgataaaagt tactgcggat ttattgctat cgtcggacgt ccgaacgttg gcaaatccac
    11881 attgttgaac aaactgctgg ggcagaagat ctccatcacc tcccgcaagg cgcaaacaac
    11941 tcgtcaccgc attgtgggga tccatactga aggcgcgtat caggcgatct acgttgatac
    12001 cccaggcctg catatggaag aaaaacgcgc cattaaccgc ctgatgaaca aagcggcgag
    12061 cagctctatc ggcgacgttg agctggtgat ttttgtcgta gaaggcaccc gctggacgcc
    12121 ggacgatgaa atggtgctca acaaactgcg cgatggcaaa gcgccggtaa tcctcgcggt
    12181 gaacaaagtg gataacgtgc aggaaaaagc cgatctgctg ccgcacttac agttcctggc
    12241 aagccagatg aacttcctgg atattgtgcc gatctctgcc gaaaccgggc tgaatgtcga
    12301 cactatcgca gcaatcgtgc gtaagcatct gcctgaagcg acccatcact tcccggaaga
    12361 ttacatcacc gatcgctcac agcgttttat ggcgtctgaa atcatccgcg aaaaactgat
    12421 gcgtttcctc ggcgctgaac tgccgtactc cgtgaccgtg gagatcgaac gtttcgtctc
    12481 taacgaacgc ggcggttatg acatcaacgg tttgattctc gttgagcgtg aagggcagaa
    12541 gaagatggtt attggcaaca aaggggccaa gatcaaaacc atcgggattg aagcgcgtaa
    12601 agacatgcag gaaatgtttg aagcgcctgt tcaccttgag ctgtgggtaa aagtgaaatc
    12661 cggttgggcc gacgacgaac gcgcactgcg cagtctcggt tatgttgacg atctttaaga
    12721 gtaactccga tggaaggctg gcagcgcgca tttgtcctgc atagtcgccc gtggagcgaa
    12781 accagcctga tgctggacgt cttcacggag gaatcgggtc gcgtgcgtct ggttgccaaa
    12841 ggcgcacgct ctaaacgctc tactctgaaa ggtgcattac aacctttcac ccctctcttg
    12901 cttcgcttcg gtgggcgtgg tgaagtcaaa acactgcgca gtgctgaagc tgtctcgctg
    12961 gcgctgccat taagcggtat cacgctttac agcggtctgt acatcaacga acttctctcc
    13021 cgcgtactgg aatacgagac gcgcttctct gaactctttt tcgattactt gcactgcatt
    13081 cagtcacttg caggtgccac tggtacgcca gaacccgcgc tgcgtcgctt tgaactggca
    13141 ctgctcgggc atctgggcta tggcgtcaat tttacccatt gtgcgggtag cggcgagtcg
    13201 gtagatgaca ccatgacgta tcgttatcgc gaagaaaaag ggtttatcgc aagcgtcgtt
    13261 atcgacaata aaacgttcac cggaaggcag ttaaaagcgt taaacgcgcg ggaatttcct
    13321 gacgcagaca cactgcgcgc cgcgaaacgc tttacccgca tggcgcttaa gccgtatctt
    13381 ggcggtaaac cattaaagag cagggaactg ttccggcagt ttatgcctaa gcgaacggtg
    13441 aaaacacatt atgaatgatg aggattgtca tggctgaatt actgttaggc gtcaacattg
    13501 accatattgc aacgctgcgt aacgcacgcg gtactgctta cccggatccg gtgcaggcgg
    13561 cgtttattgc tgagcaggcg ggagcggacg gcattaccgt gcatttacgt gaagatcgcc
    13621 gccacattac tgaccgcgat gtgcgcatcc tgcgtcagac gctggatacc cgcatgaatc
    13681 tggagatggc ggtgaccgaa gagatgctgg cgatcgccgt tgagacgaag ccacattttt
    13741 gctgtctggt accggaaaag cgtcaggaag taacaaccga aggcggcctg gacgtcgcag
    13801 ggcagcgtga caaaatgcgc gatgcctgta aacgtctggc ggaggccggg atcccggttt
    13861 ctctgtttat cgacgccgat gaagagcaga tcaaagctgc ggcagaggtt ggcgcaccgt
    13921 ttatcgagat ccacaccggt tgctatgctg atgccaaaac tgacgccgaa caggcgcaag
    13981 agctggcgcg tatcgccaaa gccgcgacct ttgccgctag tctcggtctg aaagttaacg
    14041 ctggtcacgg tctgacctat cacaacgtga aagctattgc cgccatccct gagatgcatg
    14101 aactgaatat cggtcatgcc attattggtc gtgcagtgat gaccggactg aaagatgcgg
    14161 tggcagaaat gaagcgtctg atgctggaag cgcgtggcta atggcaatat taggtttagg
    14221 tacggatatt gtggagatcg ctcgcataga agcggtgatc gcccgatccg gtgagcgcct
    14281 ggcacgccgc gtattaagcg ataacgaatg ggcaatctgg aaaacgcacc accagccggt
    14341 gcgttttctg gcgaagcgtt ttgctgtgaa agaagcggcg gcaaaagcgt ttggcaccgg
    14401 gatccgcaat ggtctggcgt ttaatcaatt tgaagtattc aatgatgagc tcggcaaacc
    14461 acggctacgg ctatggggtg aggcattaaa actggcggaa aagctcggcg ttgtaaatat
    14521 gcatgtaacg ctggcagatg aacgccacta tgcttgtgcc acggtaatta ttgaaagtta
    14581 aaaaaatacc gttctcttta taaagtaaaa cccccgaact ggtaaggaac gggggttttc
    14641 actttacaac tttaattttg tgcagcacac gattccaaca gaaatgatta tggatcaatt
    14701 tcgttatcct gtacaagcaa cttgcaatct ctgacataaa aaacgcgagc ctttacgaag
    14761 aaatcgacat tgataaaaag attaacgtga ccgctttttg ataagtacat taaaatcggt
    14821 ttcgttattg gcaattttgc tgtgctttac accatgccac tgaattcccc cattgaaacg
    14881 agtggtgtcg tcaaagctct ggtgtggagt gcagcactcg attccaataa ctcgcacgtt
    14941 cagttttggg gtgatgtaag ggctaatctg aacggctgca ttccttgttt aaggaaaaac
    15001 gaatgactga ttgccgatac ctgattaaac ggattatcaa aatcatcatt gctgttttac
    15061 agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc cggtgaacca
    15121 gtcggacgcg cctttaataa ctataaataa gtg
//
LOCUS       DAZKJR010000124        15004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKJR010000124 DAZKJR010000000
VERSION     DAZKJR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.125"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..176)
                     /locus_tag="ACHOWS_003907"
     CDS             complement(<1..176)
                     /locus_tag="ACHOWS_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049413.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPCS
                     EFFSLNYKLTKRIY"
     gene            254..1348
                     /gene="ddlA"
                     /locus_tag="ACHOWS_003908"
     CDS             254..1348
                     /gene="ddlA"
                     /locus_tag="ACHOWS_003908"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0049414.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1551..1763)
                     /gene="yaiZ"
                     /locus_tag="ACHOWS_003909"
     CDS             complement(1551..1763)
                     /gene="yaiZ"
                     /locus_tag="ACHOWS_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFQ0049415.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            2023..2331
                     /gene="yaiY"
                     /locus_tag="ACHOWS_003910"
     CDS             2023..2331
                     /gene="yaiY"
                     /locus_tag="ACHOWS_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFQ0049416.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2390..3484)
                     /gene="yaiW"
                     /locus_tag="ACHOWS_003911"
     CDS             complement(2390..3484)
                     /gene="yaiW"
                     /locus_tag="ACHOWS_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFQ0049417.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3497..4717)
                     /gene="sbmA"
                     /locus_tag="ACHOWS_003912"
                     /pseudo
     CDS             complement(3497..4717)
                     /gene="sbmA"
                     /locus_tag="ACHOWS_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            5069..6226
                     /gene="ampH"
                     /locus_tag="ACHOWS_003913"
                     /pseudo
     CDS             5069..6226
                     /gene="ampH"
                     /locus_tag="ACHOWS_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
     gene            complement(6227..6850)
                     /gene="iprA"
                     /locus_tag="ACHOWS_003914"
     CDS             complement(6227..6850)
                     /gene="iprA"
                     /locus_tag="ACHOWS_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFQ0049418.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCAGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6936..9884)
                     /gene="ehaB"
                     /locus_tag="ACHOWS_003915"
     CDS             complement(6936..9884)
                     /gene="ehaB"
                     /locus_tag="ACHOWS_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFQ0049419.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWADEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
     gene            10410..11384
                     /gene="hemB"
                     /locus_tag="ACHOWS_003916"
     CDS             10410..11384
                     /gene="hemB"
                     /locus_tag="ACHOWS_003916"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFQ0049420.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(11424..12275)
                     /gene="tauD"
                     /locus_tag="ACHOWS_003917"
     CDS             complement(11424..12275)
                     /gene="tauD"
                     /locus_tag="ACHOWS_003917"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFQ0049421.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWCWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(12272..13099)
                     /gene="tauC"
                     /locus_tag="ACHOWS_003918"
     CDS             complement(12272..13099)
                     /gene="tauC"
                     /locus_tag="ACHOWS_003918"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFQ0049422.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTFSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(13096..13863)
                     /gene="tauB"
                     /locus_tag="ACHOWS_003919"
     CDS             complement(13096..13863)
                     /gene="tauB"
                     /locus_tag="ACHOWS_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFQ0049423.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVVFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(13876..14838)
                     /gene="tauA"
                     /locus_tag="ACHOWS_003920"
     CDS             complement(13876..14838)
                     /gene="tauA"
                     /locus_tag="ACHOWS_003920"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0049424.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         3778 a         3909 c         3633 g         3684 t
ORIGIN      
        1 aaatatattc tcttcgttaa cttataatta agagaaaaaa actcactgca tggccataca
       61 agccaggaat aaattagctt cccgttcatc atttttgtga tcttgcgcac atcattgaga
      121 taacgcactt tcttcaccag tacgttgcac gctttgtcag cagaagattg caacattctg
      181 tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt gttagacggg
      241 ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta aatcagcgga
      301 acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata aaagtcgctt
      361 cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg atgccagcaa
      421 ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg cgaccagcct
      481 tgcgcaagtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg gtcagccgtt
      541 gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag atggttcctt
      601 gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc tggcttcagc
      661 agcctgtatg gacaaagatg tcaccaaacg tctgctgcgc gatgccgggc tgaacattgc
      721 gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg aagtggagtc
      781 taaactgggg ttaccgctgt ttgtaaaacc ggctaatcag ggctcttctg ttggtgtcag
      841 caaagtaacc agcgaagaac agtacgcaat tgccgtcgat ctggcgttcg agttcgatca
      901 taaagtgatc gttgagcaag ggataaaagg tcgtgagatc gaatgcgcag ttctgggcaa
      961 cgacaatccg caagccagca cctgtggcga gatcgtactc accagcgatt tctatgccta
     1021 cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag ccattgcgcc
     1081 agaaatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt tgggatgcgc
     1141 aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga tcaacgagat
     1201 caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc aagccagcgg
     1261 tctgggttac accgacctga tcacgcgttt gattgagctg gcgctggagc gtcacgctgc
     1321 ggataacgca ttgaaaacca caatgtaatt tttaaatgcc tgataacttt tattgggcat
     1381 ttaagactga tggcaaacgt aagattgcct gatgcgcttt gcttatcagg cctaccagat
     1441 aattgcaata tactgatttt gcatgatttt gtaggcaggg taaggcgttc gcgccgcatc
     1501 cggcaaaaac aacgagcact ttgtcagccc ccagaacgcc caatcctctg ttattgggcg
     1561 ttttcagttt cttcatcgcg acgacgaata atcagccccg ccagccagaa actaatcacc
     1621 cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc cagtaaaccc
     1681 accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg ccagtcacgg
     1741 cggattttta caggcaagtt catggcagct ccgttaacga aaaacaacat ccttgcatat
     1801 tctgcaacat ccgtctgtgg cgtagacaac atttccgtaa ttgttaataa atttaactta
     1861 aggttgtcat tttgtgactc acgacgaaga ttcacgcttc gggaatattc ccaacatcaa
     1921 tttaccatga ttctgatatt gacaatctta atgatctgtc agacttgcca taagccgctg
     1981 gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga tttcaccctg
     2041 tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt caacgcctgg caacattgcc
     2101 tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa cctgttagtg
     2161 catgcgcccg gcgtctatga gcgcttaatg caggtccagg aaacaggtcg cccacgggtg
     2221 gaaattggtt taggtgtcgg caccattttc gggctgatcc cgtttttagt tggctgcctc
     2281 atttttgcag tagtggcgct atggctgcac tggcgacatc gccgccagta agatttacgt
     2341 tgccagatgc ggcctaaaac gccttatctg gcatgacatt cctgtttgat cactgtttca
     2401 tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc aggttgcggg
     2461 tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt gatttacccg
     2521 ttttcgcttc ggcaagttga taaactttct tgtacagcgc cgtctcctca aaagagaagc
     2581 tgtccccttt ctctaactgg cggcgaattt cactgtcgct catccccagt tttcccgcca
     2641 gtttgcgcgt tgccagttcg gttttcccgg gctctttact gtcgtaacga attaaatcac
     2701 cgtccagtgc cagcttcacg ccgctggcct tactgaccgc gttctgaaac gcggcattac
     2761 ggctggcgta ccagccagcg ttaaaatcag caaaacggta tatcggcgcg ctatagctgg
     2821 cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcgacga ctgaagactt
     2881 cctgacggac tgtaccgtcc attttccacg gatacccttt ggtatgctgt tcggcaaagg
     2941 caatgctgac ctgcatcggc ccaccggtgc gcaccggatt aagcgaacca aacaacgtct
     3001 gccccattgg caccatgctg atcagatcgt caaaaatcgc gctcaattgc ttttcagtac
     3061 gcaccgaatc taaccgttcg ctatagcttt tgccgtttgg cgatttgatt tttagtgcgg
     3121 tatgcaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc tcttgccagg
     3181 cgatcttgct taagcccgga acagccggat ctgcctgata atttgactcc tgctgcgcca
     3241 ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt tcaaacgtgg
     3301 tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatt ttctgccgca
     3361 cgacgctcgc cacatctatc gctttttcgc cctttttcaa cggttgcgga gcctgactac
     3421 tacatcccgc taaaaccaga accgccaaca atgacagcga agatagatga ttgacgcgcg
     3481 acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg tcaccatcca
     3541 gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc agtgtggtcc
     3601 atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgtta gtaatctgcg
     3661 tcatcaggcc gagtgtaatc gtaccggcaa caattgacgg aaacagcaag aacaaaccga
     3721 aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt gaaatacata tagtgaaaat
     3781 agaggcggaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta ggcggcgtcg
     3841 cacgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcagcc tctactcgct
     3901 ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagtaat ccggtcccca
     3961 tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg acaatcggca
     4021 gctccggcac atgtgcggag agcgttacca gcaccggcag gaaggcgatc aacgtcatga
     4081 tagcgttgat aaagctgacg cccatattct ccagcgttga agcaaaacgc atggtgtctt
     4141 cctgcacacg ctgcgcggcc ccttcgatat gacgcagttg ttgccagttc gccatgtaat
     4201 attcgttcat cgccgtacgc cagcggaaca cgtagtgact gacaaagaag ttgttcagca
     4261 cactgatcac caccgcgatc agcgcaatcc ccagaaagac tcccacttcg cggtaaaatt
     4321 gttcgatggt gactttatgc ggcgaactta gcgccgtttg aatcagatca tagaacggcg
     4381 cataccaggc gttgacggca accccgactt ccaccaaaaa ccaggtgacg aagataatca
     4441 gcgcagtacc gagtattgac cagtattgcc aacggtgcgg gctatagata aaccagaaca
     4501 atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa tccaacgacc
     4561 agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc gccacccaat
     4621 cccccccacc ggcttaccag aagataacgg caatcaatgc ccaaacaaag gccgagagaa
     4681 aaaacgttcc cggctttggg aaaaaagact taaacatatg cactcctgct aacttcttat
     4741 cgttttaaca actgctgtgt attaaccgca tgaccgctac aaagcgtcag ccaaatgaaa
     4801 ccgcccattg tgacccatag caaggcgctt agttcgccag cctgagcata agaattgttt
     4861 cgacggattt cacaaaccaa agattgttta cataagcgga ttaacacgcg caggatcaca
     4921 aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg tttttaacac
     4981 tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat acgctcagtt
     5041 accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt ctgccgtgct
     5101 gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg atcactgaac cggagttcgc
     5161 ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg ccacggggat
     5221 ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgc agttatggcg aaacgcgacc
     5281 tggtaataac gttcgcccgc agctggattc ggtcatacgt attgcttccc tcaccaagct
     5341 aatgaccagt gagatgctgg tgaaattgct cgactagggg accgtgaagt taaacgatcc
     5401 gctaagtaaa tatgctccgc caggcgcacg cgtaccgact tacaatggga cgcccattac
     5461 gctggtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg gtggcgcggc
     5521 acatcgtccg gtatttgtct ggccaacgcg cgagcaacgc tggaaatacc tttctacggc
     5581 gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct aacctcgcat tcgacctgct
     5641 ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg aagagcagat
     5701 tacccgtcca ttggggatga aagacaccac ctacaccccc tcaccggatc agtgccgccg
     5761 tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg caattggcag
     5821 cggcggcgtc tattccacgc caggcgatat gatgcgctgg atgcagcagt atttgtcatc
     5881 tgatttttat cagcgcagta accaggctga tcgcatgcag acgttgattt atcagcgcgt
     5941 gcagtttaca aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc ttggtttagg
     6001 ctgggtatat atggccccga aagagggtcg tccagggatt attcagaaga caggtggtgg
     6061 tggcggtttc attacctata tggcgatgat cccgcagaag aacattggcg catttgtggt
     6121 ggtcactcgt tcgccattga cgcgcttcaa aaatatgagc gatggcatca atgacctggt
     6181 gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa tactctgaag
     6241 gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg cctttacgca
     6301 aagcagcgag aacttccgca acgacagaac gcgatatacg tgtacgttga tggatataat
     6361 tcatcacacc aatacgtgat cgcaattctt cattccagtc aatcattgat aataacgtcg
     6421 cgcggatttg ttcgtaggaa ttatgcccaa tgagctgaac gtcgcgtaat tccagaattc
     6481 tattttgcca ggctaaccag taaaaagcgt ctcgccagag ttgattttgt tcaataagag
     6541 taatggtttg tttggctggt aaatgatatc ccgcacagct tccttctgat attaatttgt
     6601 atggtatatc gtttttcatt aaaccatcag ccagccccat aatataagga gcctgggtaa
     6661 taccgataag tacgttttct tctctacgca gagagataac gccctctaaa ataacgaaag
     6721 tatcttcgct attgacatca ctggaaaata taacttgctt ttcattatta aactcgaagc
     6781 gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga ggtttaacta
     6841 cagataacat ttgtgcgtcc tttgcagtaa tgcccgtcaa atccttgacg ggcattattt
     6901 agattaaatt acctatttct tcggagtgaa gaatattacc aggtatattt aacacccaca
     6961 ttcgcggacc agtcttgatc tacgtcacca ccaccgaggt agttagcatc ggtataggcg
     7021 ctgaagttct tggtgaagct gaactgagta cccagaccaa cacgtaccgc agacccttca
     7081 gtaccgttat cgatggaatc accgttcaca tcgttatcgt tgttagagtc gtcgtagacg
     7141 taagccagtt tgaagtacgg agtcagagcc tggtcttcgc tgtaggtgaa ggtataacct
     7201 gcatctacac ccagttcata acgcatgctg tcgtaagact gaccatcaac tttcatgtcg
     7261 ttgctcagct ggtaatcatc accagactgg aacagaccag aaacgctgcc gtaaggagtc
     7321 acgtaacctg catcacccag tttgaagtcg taaccggctt tcaaaccaaa gccccaagcg
     7381 tcggagttgg tgctgccgtc aacgtaagta ccgttgctca tggttgcaga caggtcgttg
     7441 ttgaagtgag agtagctcaa gctaccgtca acaaagacgt tgttcgcgaa gtgagcagaa
     7501 gagtagatgt aggcagtctg gctgtcttga tccacctgac cagaacggtc attcatatca
     7561 cctttagcga agcctgcagc cgcaccgacg atccacttag cgttgttacc gtcaattttg
     7621 gtatcaacac cgaccatgat gccgttaaca tcctgatcat agttgatggt gccgttgtcg
     7681 ccgttgaagt taccaccgaa gtagcttacc catgcgccgc cgttatcagc cagaccatga
     7741 cgagagttgg tcagacgagt accaacggtg tcttgttcca ggttccagat attggtgttc
     7801 gcagacggga tgctcagcgc catgttagcg tagtcggtca gctccatctg ttgcagaaca
     7861 acggtgttac cacgctgctc agcctgatag gtgtatgcac ccaggtcagc tttgttagca
     7921 gcagagaagg tcgcgttgct gtttttgtcg ttgacgtaga tcagctcgtt gccgttgtag
     7981 tcagcgatag aaccagcacc agtagcgttg tcgatacgaa ctttgtagtt accggttgca
     8041 gctgcaacat ggtcaccgtt gttatcaact tcgttctgac cagcttcgat agaagccgtg
     8101 ttgtcgttat cgccgttacc gttaatggtc aggtgaccat cagagttcat tgcgattaag
     8161 ccataaccgt agtttgactt ggtagtatcg ttggtacggt cgttggtcag atcggcatcg
     8221 agtacgaagc ggttgctttc gatgttgaac acacctgcgt gaatgttacc actggtatca
     8281 gtagtgctgg tcaggttcag agtatcaacc tgcagagcat tcgccgcagt gttacctaca
     8341 tccaggatac cgtcgttagt cactttgatg ttgttagcat acagagcagc atcacctaca
     8401 gcccagccag cagaaacttc atcagaaatg gttacggtac tgttgtcgac agtcagtgaa
     8461 tcagttgcaa catgaccatc ttcgttgatg ttcagcgcag aaagaccagt cagagagata
     8521 gagtcagaaa tcagcgttga atcagcaaca ttaacagttg aaccactgtt gatgctcaga
     8581 gtatcaatca gagaagtttt ggtggtatcc cactgtgaac cggtgttcag ggtcacattg
     8641 aacagaccgc tctggtaaac ttcgttaccg atgatgtgat caaattcgct gtattcagag
     8701 tcgctgtttt ctacaccaac agttgatgaa ggccacaggc tgttagctgc gatgtcgatc
     8761 agagttgcag tggcttcagt attgattcca tatgtatagg agtcatttac accatcgtca
     8821 tcagtatcaa caccaatctg atgcgcagac attgccgcac cgacccactt gctgccattg
     8881 ttcagggtca gatccaggcg atcagtacca tcccaaccgt tagtatctac atcaccgtca
     8941 gcatcacgat agctatcacg accttgtggg aagaagttct catcaaaatt acttgagaag
     9001 aatacatcgc ccatcagtgt ggaattgtcg aatgttgcag tggtttgcat ggcgttatca
     9061 gcatatggat gtgcaataac agctaatgca acatcatcag cattccaccc atcaccattg
     9121 taatcactag cattaccagt attaccaaac caaccagtag taccttcatc agcccatgaa
     9181 ccagaggtca cggtagaatc tttaacagtg atggtgttat taaatacttc gctgctgttt
     9241 acaccagtgc tgacagtgtt gtcgtcgata tcttcccact cataaccctg agtcagcgta
     9301 atacctgcta catgagagtt attagagatg gacagatcga cttcctgatc cagagtgatg
     9361 gcagtaccaa tgttaaatac atttacaaca tgagtgtcag cggcattatt gtaagtgccg
     9421 ttatatgtgt agtgttcgta gttgtcatcg atcgttgaat tatcaacggt cagcgccaga
     9481 cgatcatggt agtagtcatc cgcacgatca gcacaatcag ttgtcatgca ctcggaataa
     9541 atcataccgt gaatagtgct gtttttgatt gtcaggctat tggcgttggt attagtagaa
     9601 ataccatcgt ccagatagta ggtagaaatc acgccgttaa cggtagcgtt attaatgacc
     9661 ggatagatat cgccgttgta ataactatca gcggcatagt tattccagcc tacataacca
     9721 tcatagtaaa cgtcatcagc ataggtggcg tcgttgtagt gatggaaagt attgtaagtt
     9781 tgaccagaaa tatcgttggt cgctgcatta gcctgagagg tgatagccag agtgcaagcc
     9841 aatgctaatt gtgatactac aagtttcttt ttccaggagt gcatttgtca tccctcctca
     9901 gggacgtaat aagttgatcc atcaattatc aatgcataga aaacatatta tgtttcgcgt
     9961 ttacgattat gcggcgttat agaggaatgg ttcaattatt ctttccccta agtccgattg
    10021 cggacatata taaagcacgt gaacacacac aaagaaagaa ataacaaaaa cacaatattt
    10081 caatacgtta acgaaagcgt taattttaaa acatagacca tgaaaatata aaacaaccaa
    10141 aacagacaaa taatgtatca ataagcaatg aactttaagt tattcacttt ttgaaaacgt
    10201 caccaaatcc ccaaaaacaa taatctatgg ctataaataa aaattaaaaa tattttcagt
    10261 gaatatgaaa ttattgctat ttctattaca aagatgtcct gacctctttt tcattctctt
    10321 cttgtcaaca tcgcgacaac tttcgtaaaa cacacccagc ctgcttcagg tatactatgc
    10381 tcctcgattc cacaaacatc aggcagacca tgacagattt aatccaacgc cctcgtcgcc
    10441 tgcgcaaatc tcctgcgctg cgcgctatgt ttgaagagac aacacttagc cttaacgacc
    10501 tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta caaagccgtt gaagccatgc
    10561 caggcgtgat gcgcattcca gagaaacatc tggcacgcga aattgaacgc atcgccaacg
    10621 ccggtattcg ttccgtgatg actttcggca tctctcacca taccgatgaa accggcagcg
    10681 acgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg catctgcaag cagactgtgc
    10741 cagaaatgat cgtcatgtca gacacttgtt tctgtgaata cacttctcac ggtcactgcg
    10801 gtgtgctgtg cgagcatggc gtcgacaacg acgcgactct ggaaaattta ggcaagcaag
    10861 ccgtggttgc agctgctgca ggcgcagatt tcatcgcccc ttccgctgcg atggatggtc
    10921 aggtacaggc gattcgccag gcgctggacg ctgcgggctt taaagatacg gcgattatgt
    10981 cgtattcgac caagttcgcc tcctcctttt atggcccgtt ccgtgaagct gccggaagcg
    11041 cattaaaagg cgaccgcaaa agctatcaga tgaacccaat gaaccgtcgt gaggcgattc
    11101 gtgagtcact gctggatgaa gcccagggcg cagactgcct gatggttaaa cctgccggag
    11161 catacctcga catcgtgcgt gagctgcgtg aacgtactga attgccgatt ggcgcgtatc
    11221 aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct ggcgggtgct atagatgaag
    11281 agaaagtcgt gctcgaaagc ttaggttcga ttaagcgtgc gggtgcggat ctgattttca
    11341 gctactttgc gctggatttg gctgagaaga agattctgcg ttaatgatca ttatgccggg
    11401 ttatccaacc cggcatttcc cccttacccc gcccgataaa acggtttatc cccaagaatc
    11461 gtcgcccgat gcattatccg ccgctgtggc aggtaatccg cattggcata gtgctgggtt
    11521 acgcggttat cccaaatcgc aatatcattt ggttgccagc accagcgcac ctgaaactcc
    11581 ggtttggtga tgtgggcaaa taaaaagccc agcaaggctt cgctctcttt ctcgctgaca
    11641 tcaacaattc gcgtggtaaa gccttcattc acaaacagcg cctgtttacc ggtcaccgga
    11701 tgcgttcgca ccaccggatg tagcaacggt ggatttttcg cgaccgcctc acgccagcgt
    11761 tgatgctcag cctcggtttt gcggtacttg tattccggga acgatttacg gaaatcatgc
    11821 tccgcacgca acccgctcaa cagctggcgg aagggaacag agagcgcctc atatgcggca
    11881 ataccactgg cccagagcgt atcgccgccg gtcgagggta actctttcgc cgccagaatc
    11941 gcccctgcgg gtggcgtttg aataaatgtc acatcggtgt gccagttgtc gttatccggc
    12001 ggattatcgt tatgggtatc caggacgatg atctcgtcaa ccccttcagc atgagggtaa
    12061 acagggtgaa tatgcagctc gccaaaacgc tgggccagcg cgcgttgctg ctgcggcgta
    12121 atagcttgat cgcgcagaaa caccacctga tggcgcagca ccgcatggta aagctgttca
    12181 aactgattat cgcttaacgg gcgcgttaag tcggcccccg taatttgtgc gccaatatac
    12241 ggccccagcg gggtaatgct cagacgttca ctcattgtac ttctccatgc cagggcgtca
    12301 ggcggcgctg taacgcgcgc agacccagtt ctaaaagaaa ggcgataatc gcaatcaccg
    12361 cgatccccgc cagcaccacg tcagtcgcga gaaattcacc cgctgactga accataaatc
    12421 ctaaaccgcg cgttgcggca atcagctccg ccgccaccag cgtagaccag cccacaccca
    12481 gaccaatgcg taatccggtg aggatttccg gcagcgcgcc gggcaaaatg acaaaccaca
    12541 gcacctgcgc acggctggca cccagcgact gggcggcacg aatgcgaacc tgctgcgcgc
    12601 ttttcacccc cgccagcgcc gacatcgcca ccggcgcaaa aatcgctaaa tagatcagta
    12661 agatcttcga ggtttcgcca ataccaaacc agatcaccat cagcggcaaa taagccagcg
    12721 gcggcaccgg acgataaagc tcgattatcg gatccagaat gccgcgcacc gtcgggctta
    12781 gccccatggc gatcccgacc ggaataccga tcaacaccgc cgcgagtagc gccagcaaaa
    12841 tgcgcgtcag gctggctgcc agatgctgcc acagcgtggc gtccataaat ccctgcggcc
    12901 cggcaatggt gagcagtttt gccagtacct gttgcggtgg cggtaaaaac agcgggctaa
    12961 tcagttgcag cgccgcgact gcccaccata cagtgagcaa aaccgctaac gtaccaatgc
    13021 tgaaggtcac ctgacgcgag agcggccagc gccatttcag ccgatgcgaa tgcagttttt
    13081 cattaatgag cacactcatg agaacgcctc ccgttgctca aatacgcggc ttaaaacata
    13141 ttcgcgcatg gcgatgaatt gtggatcgga cttgatgctg cggctcgact ctcccgcaac
    13201 aaagcggcga gcaaagttga gcggcagccg ctccagcaca cggcctgggc cgggtgaaag
    13261 cagaaccagt tcagtcgcca taaacaccgc ttcttctata tcgtgggtaa tcaacagcac
    13321 ctgctttccc gtctcctgcc agagtttcag cagcagggtt tgcatctggt cgcgggtgaa
    13381 ggcgtccagc gcaccaaacg gttcgtcgag taataacagc tggggatttg ccgccaacgc
    13441 gcgggcaatc cccacccgct gacgttgacc accggaaagc tgccagatgt agcgtttttc
    13501 tgcgccttcc agccccactt ttttcaccat ctggtgtgcg atttccagtc gctgcatttt
    13561 ctctacacct gccagttgca ggccgaacac cacgttgtct accacattac gccacggcaa
    13621 cagcccttca ttctgaaaaa ccacgccccg ctctgctccc ggtccctcaa tgcacttacc
    13681 cgccagttga atgctgccat gctgataagg cacaaaaccg gcaatcagat tcagcaaggt
    13741 ggttttgccg cagccagacg gccccagaac taccagaagc tcgccgcttt ccagcgtcag
    13801 gttgatatct tccagtgccg gtttgccgcc ataatcggcg taaagatgag aaatttgcag
    13861 catctgcgcc tccttttatt gcacgaagcg cgaggtaacg tactggctgt aatcattcgc
    13921 cacggcgggg actttgccct gctctttcaa aaactgcgcg gtgtcgatga tcgctttgtt
    13981 caccggtccg gtcagttctg ccgtttgctg ctgcggcgtc agataggtat tccccttcac
    14041 cagccccgga acgtcacctt caggcacgcc gcttaaccgc gccagtttgc tgatgttttc
    14101 cggctgtttc agccacgcgt ctgggttagc aatgtacggt tgctgagcat cgatggcgct
    14161 tttagcgaac gctttcacga cctcaggatg tttctcggca aaatctttcc gcacgaccca
    14221 gacgtccagc gtcggcgcgc cccactgccc gacctgttca gaatcggtca gcaccttgcc
    14281 gtctttttcc agcgcgttaa ccgccggtgc ccagacataa gcaccatcaa tatctccccg
    14341 ctgccaggcg gcgataattg cgggcggctg caggttcaca atctccactt gcccaggttt
    14401 aataccccag tgtttcagcg ccgccagcag gctgtagtgg gtggtggaga taaacggtac
    14461 ggcgatgcgc ttgccaatca gatcttccgg tttgctgata gttttcttca ccaccagcgc
    14521 ttcggagtta cccagttttg acgccagtaa gaagacttca atcggcacct gttggctggc
    14581 tgccaccgct aatgggctgg aaccgaggtt accgatttgc acgtcgccag aagccagcgc
    14641 ccgcacgatg ctggctccgc tgtcaaactt acgccagtca acgctcgccc cgctctcttt
    14701 agcaaaggtg ttgtcggcct gagccacttt cgccggttcg gctgaagttt gatacgccac
    14761 agtgacgttc accgcctgtg cctgaaaagc gatgaatgcc agtgcggcaa gaagtgtgtt
    14821 acgcgatgaa attgccatga ttgtctgctc ccctgtcttg ttatgggagc agtattcagg
    14881 aataaaaaca ttcattaaaa gaattagttg ttatcgcaca gatgatttta ttcttagcga
    14941 aaaagtggca aaggaaagat taaatatgca attacaccca ctctgacaat tcagtaagtt
    15001 actg
//
LOCUS       DAZKJR010000125        14823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKJR010000125 DAZKJR010000000
VERSION     DAZKJR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.126"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(248..1120)
                     /locus_tag="ACHOWS_003921"
     CDS             complement(248..1120)
                     /locus_tag="ACHOWS_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFQ0049425.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            2461..3951
                     /locus_tag="ACHOWS_003922"
     CDS             2461..3951
                     /locus_tag="ACHOWS_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFQ0049426.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGESLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(4073..4645)
                     /locus_tag="ACHOWS_003923"
     CDS             complement(4073..4645)
                     /locus_tag="ACHOWS_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049427.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(4731..4937)
                     /locus_tag="ACHOWS_003924"
     CDS             complement(4731..4937)
                     /locus_tag="ACHOWS_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0049428.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            5794..6018
                     /gene="hha"
                     /locus_tag="ACHOWS_003925"
     CDS             5794..6018
                     /gene="hha"
                     /locus_tag="ACHOWS_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFQ0049429.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(6350..6547)
                     /locus_tag="ACHOWS_003926"
     CDS             complement(6350..6547)
                     /locus_tag="ACHOWS_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049430.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            6746..7315
                     /locus_tag="ACHOWS_003927"
     CDS             6746..7315
                     /locus_tag="ACHOWS_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFQ0049431.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            7576..7977
                     /locus_tag="ACHOWS_003928"
     CDS             7576..7977
                     /locus_tag="ACHOWS_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFQ0049432.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            7965..8399
                     /locus_tag="ACHOWS_003929"
     CDS             7965..8399
                     /locus_tag="ACHOWS_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0049433.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            9174..9400
                     /locus_tag="ACHOWS_003930"
                     /pseudo
     CDS             9174..9400
                     /locus_tag="ACHOWS_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(9402..10184)
                     /locus_tag="ACHOWS_003931"
     CDS             complement(9402..10184)
                     /locus_tag="ACHOWS_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFQ0049434.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            10487..11407
                     /gene="rbsK"
                     /locus_tag="ACHOWS_003932"
     CDS             10487..11407
                     /gene="rbsK"
                     /locus_tag="ACHOWS_003932"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0049435.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            11435..12751
                     /gene="fucP"
                     /locus_tag="ACHOWS_003933"
     CDS             11435..12751
                     /gene="fucP"
                     /locus_tag="ACHOWS_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFQ0049436.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            12763..13776
                     /locus_tag="ACHOWS_003934"
     CDS             12763..13776
                     /locus_tag="ACHOWS_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0049437.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            14198..14482
                     /locus_tag="ACHOWS_003935"
     CDS             14198..14482
                     /locus_tag="ACHOWS_003935"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0049438.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(14698..>14823)
                     /locus_tag="ACHOWS_003936"
     CDS             complement(14698..>14823)
                     /locus_tag="ACHOWS_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0049439.1"
                     /translation="LLITFLPVFLLPVLGDIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT         3840 a         3301 c         3730 g         3952 t
ORIGIN      
        1 gagatccggc tgacggaagt ttctgaattg tggttctgtt gttccctgaa tctcatgtct
       61 gtgtgtatct gcgcacgttg ctaacaggag ttattattag cataacgatc gatattaacg
      121 atcggtgaaa tgggtatcga tcgttttatt ctattatcta gctattgcac ggacaagaga
      181 gacagtaacg aaaacagcgc ttttgtgagc agacccagag gcagggagga catcatccac
      241 gacaggttca gaagaaaatc cagttccata ccgcacgggc aacagagacc accgtgtcac
      301 gaacagcgcg cagggccgta cggacaacag aggcaacaca gacgctctcc gctgtgtcaa
      361 atatccggtc caccgcaccg gtaaactgtt cacgggcctg agagcggaca gattccgtgc
      421 gcagcccgtc ctgcagtcgg gtcatcaggg gactggcggc atggtcggga agcgctgtca
      481 tgagcgcact gaccagcgta tccagttccc agccggtgcg ggccgatacg gccacaaccg
      541 gatgtacggg ccggaacaga cggaataccg cctccgtttt ctcacgaatg ttctgttcct
      601 gtgcgggaga aggctgaatg ccggccatat cccattcatg gcagggctcc gttttgtcgg
      661 cctgcgtcac cacaaacagc acctgctgat gtccgcactg caggatgtgt cgccagaaat
      721 actcatccac agacagggcc cggtcatcgg ctttaatcag ccacagcacc aggtcaagtt
      781 caggcagaat gtcacggtac agggcctcat actctgcatc cctgtcccgg ctctcgccca
      841 ccccgggcag gtcagtgata accatgctgt gaccgtgacc actcagacgg aagcgccgta
      901 cttcccgggt gccggcgtga acatcactga ccggggtgac ctccccctga aacagtgcat
      961 tacagagtga ggatttaccg gccccgcttt tacccataat gccaatcacg ggttcgtgac
     1021 tggtgagttt acgcagatgt tccaggatgt gacgggaaag tgagtaaggc agggaggaaa
     1081 gcggtttttc aattgcctca atggcatcag acggattcat acatgtattc ccggtgaaaa
     1141 aagacaaaac cccgcgcacc ggaaaggtgg cgggggatcc acggagataa tatctgtctg
     1201 aaattgtttt gaatcgatgg gattgttgcg tattaacatg aaggagatca ggacttatcc
     1261 gttactgggt gatctccttc catgaaaata cgcggttatt gttaaacagc aggctgatta
     1321 ctccgtaaag gagaaaaata ttcatcttgc agcctgatta tcaggtgtgt ttatgtttac
     1381 tgaggtgagt atttttcagt atattgtcgg ggcagtcagt gggagatatt aaatttacgt
     1441 ttagagttat cttgcttctg ctgtgtattt ttataagagt gcgcacgtaa tgatatctgt
     1501 tgataccatg ttgttaagtg tttcccttcc gggtcatgcg tacttgcaaa atggcatctg
     1561 aatcgatcac ggtttggcca aaccgtgatc gaatagagag gaatacgcac gtaatgatta
     1621 aatgcttttg ttacaccatt cttccggcat gaatatttat ccagttccct tttttatttg
     1681 tgtagtgata ctgtcggaat agcgttcagt aatgtgaagt gtcctgttaa aaatctttaa
     1741 taaatgtgtc accctggtca ggaaatatct gctgagatat tattagttgg tggtgttttt
     1801 actgagtaat agctggcttt taattcctga actgaaatgc tgtcgctcgt cagggtttaa
     1861 ttaaaactgg atgatgatgt gaaaatgaag gctggttttt gtgtttgtcg aggaagtgtt
     1921 gtttttatcc attctcatca ttggctggct gcgattatac agaagtattt gaagaaaaaa
     1981 tggtgcattt tatctgtgcc ggcagggagg aggattttcc tgatatgtca gtattaaatg
     2041 gtttttgtta tttataaaaa tgtcatcctg tccatagaat attgtactga gtactcagca
     2101 ttcagcgcag gaactgtcat cttctttatt ttattcttct tcaggagggc agttttctga
     2161 aagcgttact ccggggaaac tgtttatgtt tattcccggc aggaaacccg tacctgcaat
     2221 accctggatg atgttttttg ctggtacgtt gttgtgtgaa aattaatcaa catgtgtgct
     2281 ccggagagcg cccgtagacg cacagattca ttctgacatt ctttagcgac aaaataccgg
     2341 gtggttatta tttaacctga aaatcgacag aaatttttat cctgtattga ttctgctgct
     2401 atttttgcta ttagtagcaa tagttgcggt actttatgtt ctgtgtgttg tgaggtcatt
     2461 atgccatact ttcagtatcc tgatgagttt cccctgagct cgctgcctcc actcattcgt
     2521 gatgccgtta ttgaagctca gcaaatcacg caggctcccc tggggcttgt ggcagcatct
     2581 gccctgggcg ctgtttcact tgtctgccag aatctgattg atgtctgcag actgaataca
     2641 ctgcgggggc cggtttctct tttcttactg acgcttgctg agtccggaga acgaaaaacc
     2701 gcagtggaca aattattaat ggagcccctg taccagcagg agatgctgtt atattccaga
     2761 cacaaaaatg aactgacgac atggaaaaat aaggaagagc ttttaaaggc gcagaaaaaa
     2821 gcattgttgt caaaactgaa taaagaactc cgcaaaggcg cggatgagtc agaaacgctc
     2881 agacaactgg aagcgcttca gaaaaacaga ggggaaaaac ctgtaagata taaattcatt
     2941 tttaatgatg ccacgacagc agcaattaaa gatcagcttt gtggtcagtg gcgttcagtg
     3001 ggtatcatgt ctgatgaagc cggtataata tttgatgggt ataccctgtc tgagctgcct
     3061 tttattaaca aaatgtggga tggctccgta ttgtcggtgg acagaaagaa tgaacccgaa
     3121 cagatgattg aaaatgccag aatgacatta tccctcatgg tacaacccgg tctttttgac
     3181 cgatatatgg agcgaaaagg ttctgttgcc agggacagtg gttttcttgc ccgttgtcta
     3241 atcagtaaac ctgccaccac gcaggggaaa cgctttatta acggagcagt gaccccggga
     3301 gaatcattaa cggcatttca tgaacgatta atggaactgg ccagaggcag tattgaaaaa
     3361 tccagtaagg atgaacgtta ttgtctgcat ttttctccag aagcgcaaaa aatatttatt
     3421 gatcactata atgttctgga gcaggatttg tctccttccg gacccttaag ccagtttcgg
     3481 gggcatgttt caaaaaaaac agaaaacatt gcgagaattg ctgcgttatt gcagtatttc
     3541 agtcagggtg aagggaaaat ctctgccgat atcatgacgt ctgctgtcgt aataagttcg
     3601 tggtacacag atgaatataa aaaattattt gcgttacctg atgaatctga actgcagctg
     3661 caggacgccc gggagctgct cgactggctc attgaggagt gccgcggaga atgcccgccc
     3721 cgtgtcagga aaaattatat tctccagtgt ggcccgggcc ggttcaggaa caggaaaaag
     3781 ctgaacgtcc tgctgaacat tcttgcatct caactcaggc tctctgtcgt gcaggaagga
     3841 aaaaccacct atgtcctgct ttccgaaata aacaatatca cgctgcctga actgaatgcg
     3901 tgctacagcc agaaactgat aagatggcat gagcaatcct ttggcttata atgttccctg
     3961 ttgctgagac ggattattca gtggtggtat cgcagtatgc ccgtcacccc ccgcttctgc
     4021 atgagggtga cggaggaaaa gtaagtgtgc ggaggcccgg gagcgttatg aatcaacagg
     4081 cgtataacag gtactcgtca atcatcagtt ccagctcgcg cttttcccgt ttgcgcgcca
     4141 cagcccagtt ttcaagctct gcatgtgatt tatgctttaa ctcttcagtc cagggctgca
     4201 tggagtgggc acacatcaga tcataccagc acagccatat cagcgtgtgg tagagatcac
     4261 gatcgtcgaa acgccggagc aggctgttca gcctgtttgc cagccggttt tcacccagac
     4321 gatgtagctc actgagtaaa acgccgccca gattaaaatg tgtaagcagc atgatttcat
     4381 gggtctgaat gctggtcatt aacccttcct gaatggcagg aatgaacggt ggtgttcccg
     4441 ggttgacgac ctgatagagt ccctgtggtg ccactgagat gatagtgcct ttttccggca
     4501 gaaaaaagtg ggccagggtc aggcgatgat aaccatgaag atgcggagac cccattccat
     4561 gagcgacgat aagcctcgtc acgcttgtca tccatttctg atgctggatg agcagcagct
     4621 cacaccggtt ttcaagtttt tgcataatat tctctccttt agcgcgcagc aaaatgcctc
     4681 cgggggccgg atcatgcctt tgctgcggtt gaacggataa cggtcaggaa ttacggacgg
     4741 gactgtgcaa tacgcgcctg cagccaggtt tccacttcac ttttcagcca tcgggagcta
     4801 cggcccagtt tgatgggggc aggaaaggca ccatccttga tgagcttgta aaaccattta
     4861 tcggtcaggc cggtcagttg agtgataaat gccatgtcga ccatctggtc atccatcagt
     4921 gaaactgggg tattcatgag aatgttcctt cggatgttgt taatctcaac cggcgttggt
     4981 gatgaaaaca acgcaccacc ttcaccggca taacaaccgg ggagaaacgt aaaaaataat
     5041 cgggaatgtg accgtcagga gataaacggg gggattgcgg tggctgagcc gtaccggtat
     5101 gtcgggtgat tgttgcgaaa cgatgcttcc gcacgcgcac gggaggactt tcgtgagaca
     5161 aggggaaaac tggcgttctg ataattgcag agttgttttt gaaggcggtt gtggtgcatc
     5221 agcctgtgac ctgtgggcag gcgacaggta tcactggtgg gataagactg tctgattggt
     5281 attataaata tataagagag ctcaaacaca tgagttcata tatgtgaaat aaatctgtga
     5341 gtgtgatcag cagaccagtc tacccagtag caacagattg cgttgtactc acagggttat
     5401 ccctgataac tggcgtataa cctgcgtaca taaaacgtac ctgcaatgca cctgaacaga
     5461 gtgcattaag catccgcctg agcggtaaac gtatccgatc tggtgagtga cagtctgaga
     5521 gcattctcag taatcgcccc cttgtatcct ggaaattggt taagagcgat tagatttggc
     5581 aaggtgtatg catccagcag cttaacacct cagctaactg gctggttacc ttcaggttgt
     5641 cgcaatggca tcggacatcc ggtgtcggat aactaaacgt agcgttaagg cgatattgct
     5701 gagagaccgg ttaagtggtg gcacgggagt aatgagtttg gggagggtac cagaatgcca
     5761 gtcaggtgcg gtatgctggc agtatcacgt tgattgcagc ataaagtaac gagagacgaa
     5821 tggaagttaa aaccagagca gttccgtcgt tgttcatccc gggagacgct ggaaaaagtg
     5881 attacccgca cacgttataa acttacccct gcggagctgg aagcctttaa ctctgcggtc
     5941 gatcaccggc tggcagaact gacaatgaac aaactttacg atcgcgtgcc ggcttccgtc
     6001 tggaaatatg tcacctgaat cttgaatccg cgctacgatg actctggtgt tatctgtgca
     6061 ccggaagatc gggaacatta agcagtataa cggcaacaaa gaattcgaaa tcaaaaggga
     6121 atggtgtaca gaggggcagg cgttcaggca ttaccggcct gtatcttctg aaggggactc
     6181 gtatttgtcg gggctgagta ttccgctcga ttcgatcacg gattggcaga aacctgactg
     6241 tgattgattc aggtaccatt ttgcagggat ccggtgcgtt actgtctggc acaacaaacg
     6301 cagcgggtcg ctctgccccg ccgattcctg ataattcctc ccctgtaacc tactgcaaca
     6361 gtacaaatac actgacgcct gaaacggtaa ccagaatcag caccagcagt aacgtcagta
     6421 aacagagcag acgatcggtc ttccggtaat aaccgaaggg gtcgacagcg ccacaatacg
     6481 ggcaacacac tgtattatga gtaattgcgt ttccacagtc cctgcacttc tttttgcgta
     6541 tcacgatcgc tccctctcca cgccttatcc gtaacctgtt ttttacatta aaaaaacaac
     6601 gccgggaatt caatcgtcag tccggagacg gccgttcggg ttatcacaga gtgcctgaga
     6661 cagtgtcctg ccggaggtca cctcaggggc tctgcgcatt ttttacgacg tggctattcc
     6721 gtatgaacca tacggagatt taaccatgac ctacaaatac aaccccttct ggcagcaacg
     6781 tattcgtgag acggtgcggc acgcactgga tgttcatccc cgcctgacgg cacttcgggt
     6841 tgatctgcgt ctcccggatg tgccggcagc aacggacgcg gctgtgatat cccgcttcat
     6901 caatgccctg aaagcccgaa tcgacgctta tcagaaacgt aagcatcggg aaggtaaacg
     6961 cgtgcattcc tcgaccctgc attacgcctg ggcccgggag ttcggggagc ttaaaggcaa
     7021 aaaacactat cacctgctgc tgctggtcaa ccgggatacc tggtgtcgtg ccggcgatta
     7081 ccgcgctccg gggtcactgg ccgggatgat taaacaggcc tggtgcagtg ccctgggagt
     7141 ggatgccgga cgttatgaca cgctggcaca ttttcctgac aagccagcgg tgtggctgga
     7201 acgcgatgat gaaaccggct ttcagcaggt gctggaacgt tctggctatc tggcgaagga
     7261 gcataccaaa gctcgcggca ccggtgagcg caattttggc tgtagccgga gttgaacacg
     7321 acaggcgcac caatacccgg caaaccaaaa gtatgggttg ttgtctggcg gcaacgatgt
     7381 atccactcgc ccactgactg ttttttacgt cagtgggcca ttgtgtgaca accacagtgc
     7441 tgtaacagca accagcctgc tttacccaaa ctccagtcca gattttaact gaaccgccat
     7501 tcacaccctg accacgctgc ccgtaccggg acggctgttg cctgcgtgtc gtctggcggc
     7561 tgaggaacat atactatgta cgcaaaatcc tttctcgctc ttgatggcaa cggacgtctg
     7621 acgggggccc gtaccgcaca gactgcgcct tacgaccgct acacctgcca cctttgcggc
     7681 agtgcactca ggtaccaccc gcaatacgac actgaacgtc cctggtttga acacactgac
     7741 gacgggctga ctaagcacgg tcatgagtgc ccttatgtca gaccggaacg cagggaggtt
     7801 cgcttgatta agcgtctgca gcaattcgta ccggatgcct tacccgtggt gcgtaaagcc
     7861 agctggcact gcagacaatg tcaccacgat tattatgggg agcagtactg cacaaactgc
     7921 cagaccggag gattcagtat tccccggaca actcaggagg aaatatgcga attctgaact
     7981 gttatatggc gaatgacagc aaaggccatt ttgttacggc gaaagaagct gcgaagcaca
     8041 accgacagga cgttttgtgc tgtgtgtcct gtggatgccc gttaacactt cagcggggca
     8101 atgacggaca accaccgtgg tttgaacatg accagatgac tgtcgctgaa aaaatcctgc
     8161 tgcgatgtac ctggcttgac ccggcagaga aagaggcccg tcgtttgcat ctgcagggca
     8221 tgacggttcc ggattatacg gtgaaggtga gaaagtggtt ttgtgtgatg tgtgacgaag
     8281 attatgaggg ggaaaagtgc tgcccacgct gcggtactgg ggtatacagc agggcgtggg
     8341 ggcggcagga ggtgccgtcg gaagatgcca gggctgataa tccgttacag aggctgtaat
     8401 ggttgcctcc ggagcagttt gcggtaatgc cgttcccggg aatggattgc cagtgcgggg
     8461 actgtggagc ttaattccgg tgttggtggc ctttccgttg attttgagcc aacagccctc
     8521 ctgtgactga caggctgcct cgtcattcca ttcgtatctc cagtaacagg gggttgtatt
     8581 gtattttaat gtgccggttc aggcgtgcgt ttcccggcgc gtctgcaccg gcttaaccaa
     8641 attcaacagg gataaaatag tgtaaataac cgaagtatta cttgcagaat cgggggaagc
     8701 atccggggtt ttattcaaca ggcatgggag gctccggctc tgacagacat tgtactgtgg
     8761 aatgttgtta caggttgggg cggcaaccgt gccaggagac catgagccgt tcacggcatt
     8821 aaaggccaca cttcggtcgt ttacattccc tgtggtggca ggcatcttcc gttaaggcgt
     8881 cagagcgtta ccaacgttat gttgtcatgg ctcacctttc atagtcgatg cttatctggt
     8941 gactgaaact agccgcagat aaagagtgtg aagttgatac catggagtgg agtgacgtgc
     9001 aggcaggaga ttcgctctat gcagggttgt gagatgaatt caaaccggct gtcagcgcca
     9061 gtaatatttg aagattcgtc cggttgttat ccagtgtgca ttaaaaaccc agacattatg
     9121 gacaatacaa cggcttccat aacagggagg agcctgttta ttatccgggc gtaatgacta
     9181 acctttgtgg gctttttccc gtcgtgcttt taacgttgaa ggcattttct gcctgccgat
     9241 tcaggacagc gacaaaagcc atatcagaca attggtcaat gatgaccagc gtctggagta
     9301 gatgataagc caaattgata aactggaata tgtgatgaaa gtgcaacgta atcagtccaa
     9361 tccgacgatg tttaacaaga gtacggtgtt ttttcagtaa gtcattcgtc ttctgaatca
     9421 ggtgtaatga tctttacaga agcattccgg cagtgcgtaa cataatctgc cggtaacgga
     9481 cgctcgctaa tcagaacatc aaatgcagag agcggtgcga ggctggcaga ggctacttca
     9541 tcgaacaagg cgtggcgggc gagcaaaatt ttccttagtc cacgggccat cgctttacgc
     9601 ttagtggcaa gatcttcggg attaaaccag ctgacgccaa agtgatcatg tacaccgctg
     9661 gcggaaataa atatttttcg cggattgaga gagtctaatg gcgaagagtt actggcatcg
     9721 taaaaagcat cacttctggc acgatacgta ccaccacaaa gtattgctgt cacattaggt
     9781 ttttcattca acgcaacaaa gacacgatgt gagtaacaga tgccagtgaa ggtgattgca
     9841 tccgggatca tgcttataac gagcggtatc tcctggccat tatcaaagaa gatcagatca
     9901 ttttcattaa ccattccggc ggccagaatt gcaataggta agtcatcacg atgatttctc
     9961 ggaacgtcct ggattactgg catggatggt gcgggtttat gcaccattac aatatagcca
    10021 cccagtaggg tcagtggcag aggttcatct tcctgatgga gatcgcgacg aatagtcatt
    10081 acagaaactt ccagcattcg tgccgcgtct ttcaaatgga ttctgtcggt tttcttaagt
    10141 atttcaatca aacgtcggat acgctctttt tgcttcgttt ccatcaactc tctccgtaaa
    10201 atgttataaa aataacatta tggcattagt aaacattgtt ttagttacca tactatacga
    10261 ccgacccttt gaaatgaaaa gggtatagac tgctgaattt tcggtgttaa tcctgttagg
    10321 ttgcttatga ttagatagtg ttggcctgag tgataagact ggaagtctat taaataaaca
    10381 tcgggttgat gtgctaatcg cgatctcagt cacaaatgat gcaaaagtaa catgctaatg
    10441 ttatttaaat accattgtta tgcgaaagtt tataagaggt agtgaaatgg acattgcagt
    10501 tattggttcc aatatggtgg atctcattac ttacactaat cagatgccta aagaaggaga
    10561 aactctggaa gcacctgcct tcaaaatcgg atgtggtggg aaaggtgcta atcaagcggt
    10621 ggcggcggct aaattgaatt caaaagtcct gatgctgact aaagtaggtg atgatatttt
    10681 tgcggataat actattcgta atctcgaatc atggggaatt aataccactt atgttgagaa
    10741 agtcccctgt accagcagtg gtgttgcacc gatctttgtt aatgccaact cgagcaatag
    10801 tatattaata attaagggag cgaataaatt tctttccccg gaagatatcg atcgagctgc
    10861 agaagatctg aaaaaatgca aattgatcgt tttacaactg gaagttcaac tggaaacagt
    10921 ttaccacgcg attgagtttg gtaagaaaaa tggtattgaa gtgttattaa atccggctcc
    10981 ggcattgcgt gagttagata tgtcttatgc ctgcaaatgt gatttcttta tcccgaatga
    11041 aacagaactg gaaatattaa ccggtatgtc ggttgatact tatgatcata ttcgtttggc
    11101 cgcacgttcg ttagttgata aagggctgaa caatattatt gttaccatga gcgaaaaagg
    11161 cgcattatgg atgacccgcg atcaggaagt ccatgttcct gcattcaaag taaatgctgt
    11221 tgatactagt ggcgctggtg acgcatttat tggttgcttt tcccattatt atgttcaaag
    11281 cggtgatgtt gaagccgctt tgaaaaaagc cgctctcttt gccgctttca gcgtcacagg
    11341 gaaaggtacg caatcatctt atccgagtat tgaacaattt aatgaatttt taaccttaaa
    11401 cgaataatca tcctgcacag ttaaaggtag cactatgaac gataaaaaca tcattcagat
    11461 gcctgatggg tatctgaata agactccctt gttccagttt attttgttat cgtgtttatt
    11521 cccgctatgg ggatgcgcag ctgcattaaa tgacatatta atcactcaat tcaaaagtgt
    11581 attttcgcta agtaactttg cttcagcatt ggtgcagagc gcattttatg gaggttattt
    11641 tttaattgca ataccagcat cccttgtgat taaaaagacc agttataagg tggcaatttt
    11701 aattggcctg acgctgtata ttggtggttg tacacttttt ttccctgcct cgcatatggc
    11761 aacctacact atgttccttg cagctatttt tgcgattgcg attggcctga gtttccttga
    11821 aactgcagcg aacacataca gttcaatgat cggtccaaaa gcctatgcca cattgcggct
    11881 caatatcagc cagacctttt atccgattgg tgctgcgtct ggtattttat tgggcaaata
    11941 tctggttttc tccgaggggg aaagccttga aaagcagatg tcaggaatga acgccgggca
    12001 aattcacaac tttaaggtgc tgatgttaga gaacaccctt gaaccgtata agtatatgat
    12061 tatgattctg gttgtggtca tggtgttgtt ccttttaacc cgcttcccaa cctgtaaagt
    12121 ggcacagaca agtcaccata aacgcccgtc agcaatggat acgctgcgct atctggctag
    12181 aaatccgcgc tttcgtcgcg gtattgtggc gcagttcctt tatgtgggta tgcaggtcgc
    12241 agtatggtca tttaccatcc gtctggcgct ggagttgggc gatatcaacg aacgtgatgc
    12301 ttcaaacttc atggtctata gctttgcctg cttctttatt ggcaagttta tagccaatat
    12361 ccttatgacg cgttttaatc cggaaaaagt attgattctc tattccgtta taggggcgct
    12421 gttcctggca tatgttgcac tggctccaag tttcagcgcg gtctatgtcg ccgtcctggt
    12481 aagtgttttg tttggccctt gctgggcgac gatctatgca ggaacactgg atacagttga
    12541 taacgaacat acagaaatgg caggagcagt cattgttatg gctattgtcg gtgccgcggt
    12601 ggttccggca attcagggat atattgcaga tatgttccac tcactacagc tttcattcct
    12661 ggtttctatg ctctgctttg tctatgtcgg agtctacttc tggcgtgaaa gcaaagtacg
    12721 taccgcactg gctgaagtca cagcgtcctg aggagaaccg ttatgagtac gcgtattaat
    12781 ttgtggcggg cgctttttgg cgagaaacct cgtatcttgc tggaaaatag tgattttact
    12841 gtcacgtctt ttcgatatga cagcggtgtc gaggggctta aaattgcaaa cagtcgtgga
    12901 catttgatta ttcttccctg gatggggcag atgatttggg atgcgcaatt tgatggacac
    12961 ggcctcacca tgtgcaatat gtttcgccag cctaaaccgg cgacagaggt gatagagacc
    13021 tatggctgtt ttgcttttca ttcaggtttg ctggctaatg gctgcccatc tgcggaagat
    13081 actcatctgc tacacggtga aatggcctgt gccgcgatgg atgaagcatg gatggaatta
    13141 gagggagata tgttacgcct taccgggcgt tacgagtatg taaaggggtt cgggcatcat
    13201 tatctggctc agccaacagt ggtccttcac aaatcctcta cactgttcga tattaagatg
    13261 gccgtaacca atctggcttc tgtagatatg ccactacaat atatgtgcca catgaattat
    13321 gcatatatac caaatgctac gttcagtcag aatataccgg acgaaatatt aaggttgcgt
    13381 gaatcagtac cttcgcatgt taatcccacc actcagtggc tggcgtttaa tcaacggatt
    13441 atgcagggag aagcctctct gtcgacgctg aatcaaccgg agttttatga tccagaaatt
    13501 gtcttctttg cagacaaact ggacgcttac acagatcagc cagagtttcg catgatcgct
    13561 cccgatggca ctacgtttgt tactcgtttc tccagtgccg aactaaatta tgtgacacgc
    13621 tggatccttt ataacggaga gcaacaggta gccgcctttg cattacctgc aacctgtcgg
    13681 cctgaggggt atcttgccgc gcaacgtaat ggcacgctga ttcaggttgc accgcagcaa
    13741 acgcgcactt ttacagtcac cacaggaatt gaataaccac tcaaggcctg aacagcggcg
    13801 cgcttatcgt taaggtaagc gcgttttttt acccgccagg acaagactat gatagcgttg
    13861 cccttataga cgatcacatt atcgtccact ccaaatttgc gcaactactg tagagtggtg
    13921 tactccacag cttctttgag gagcttaacc tccattgttt tcttaccgag caagcactgc
    13981 aactctttta tttatttgta gtggtcaaca aaaactggtc acaatttcag tttttttcca
    14041 gtatctattt tccgattcgt tcggcggtaa tccaccgtta tattcatgag gccggagtgc
    14101 actataaaag tgtatgcgta aaccgactgc tgtatgtagc cattaagcca gtattggtaa
    14161 cataggtacc caccttttca actagtggac acctgttatg gaacaaaaaa cattatcagc
    14221 tgaaccccgc aggtcatttt caaatgagtt taaacttcaa atggttaaac tggctttaca
    14281 accaaggggt tctgttgccc gtattgcccg ggagcatgat atcaatgata atcttctgtt
    14341 caaatggctc aggctctcgc tgatcaaagg gcgtatatca cggcgtcttc cggtaacgaa
    14401 ctcttctggc attggcgttg aattattacc tgtggagatg acgctggatg agcggcctta
    14461 ccccgttttt tacgcgaggt aattatgaag atcaatgact agccgtcgaa ccgtgtgcat
    14521 gaactgctgc actgaaactt ctcagctgta aaataatcct tacgctacgt acttcccgga
    14581 gcgcttacga ttaatcggat gcagtcctga ctggaagaaa gaaaaatgga gaaaataaga
    14641 ggaattaata tcctccacgt tcctgaaatc ctggaggata ttaatgtcat caaaatatta
    14701 gcgaacgata atccctaaca aaatacccag tgcgccaaag tcagcatcac tgaaggtggt
    14761 gttagcaaaa ccaatatcac ccagcaccgg caataagaat actggcagga aggtaataag
    14821 caa
//
LOCUS       DAZKJR010000126        14592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKJR010000126 DAZKJR010000000
VERSION     DAZKJR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.127"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..341
                     /locus_tag="ACHOWS_003937"
     CDS             <1..341
                     /locus_tag="ACHOWS_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049440.1"
                     /translation="LVSNKTIRTEKIDVVVRLAGLKAYDLQEGVPDGLKWKVKDGINE
                     RIKTDTSLCLPQDISDTSLNRYRHAVTRIVNDEIDTLLMEQKKKNKRFPYSKDRHAIQ
                     PLWSKCVIEQ"
     gene            complement(434..1030)
                     /locus_tag="ACHOWS_003938"
     CDS             complement(434..1030)
                     /locus_tag="ACHOWS_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049441.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETVATKAGVILLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            complement(1160..2476)
                     /locus_tag="ACHOWS_003939"
     CDS             complement(1160..2476)
                     /locus_tag="ACHOWS_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0049442.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQSSGSK
                     IFYFRYYVEKGTKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQTELERQQLAE
                     QQRRQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSVGISFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRLCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELSCVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRACNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
     gene            complement(2773..3537)
                     /gene="nfeF"
                     /locus_tag="ACHOWS_003940"
                     /gene_synonym="yqjH"
     CDS             complement(2773..3537)
                     /gene="nfeF"
                     /locus_tag="ACHOWS_003940"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFQ0049443.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISVGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            3826..4449
                     /gene="nfeR"
                     /locus_tag="ACHOWS_003941"
                     /gene_synonym="yqjI"
     CDS             3826..4449
                     /gene="nfeR"
                     /locus_tag="ACHOWS_003941"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFQ0049444.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(4553..6073)
                     /gene="aer"
                     /locus_tag="ACHOWS_003942"
     CDS             complement(4553..6073)
                     /gene="aer"
                     /locus_tag="ACHOWS_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFQ0049445.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLTAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            6491..7870
                     /gene="ygjG"
                     /locus_tag="ACHOWS_003943"
     CDS             6491..7870
                     /gene="ygjG"
                     /locus_tag="ACHOWS_003943"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFQ0049446.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AMRVSVEEA"
     gene            complement(7912..8244)
                     /locus_tag="ACHOWS_003944"
     CDS             complement(7912..8244)
                     /locus_tag="ACHOWS_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFQ0049447.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            8463..9446
                     /gene="ebgR"
                     /locus_tag="ACHOWS_003945"
     CDS             8463..9446
                     /gene="ebgR"
                     /locus_tag="ACHOWS_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFQ0049448.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            9630..12722
                     /gene="ebgA"
                     /locus_tag="ACHOWS_003946"
     CDS             9630..12722
                     /gene="ebgA"
                     /locus_tag="ACHOWS_003946"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFQ0049449.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGHAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENNLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            12719..13168
                     /locus_tag="ACHOWS_003947"
     CDS             12719..13168
                     /locus_tag="ACHOWS_003947"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFQ0049450.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVIYDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            13231..>14592
                     /locus_tag="ACHOWS_003948"
     CDS             13231..>14592
                     /locus_tag="ACHOWS_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0049451.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKY"
BASE COUNT         3625 a         3813 c         3539 g         3615 t
ORIGIN      
        1 tgttggtaag taataaaaca atacgaacag agaagattga cgttgttgta cgacttgcgg
       61 gactgaaagc ctatgatttg caggaagggg ttcctgatgg tctgaaatgg aaagttaaag
      121 atggcataaa tgaacgcatc aaaaccgata ccagtttatg cctgcctcag gatatcagtg
      181 atacatcgct aaaccgttat cgtcacgctg tgacgcgcat tgtcaacgac gaaatcgaca
      241 cattactgat ggaacagaag aaaaaaaata aaagattccc ctacagcaaa gatcgacatg
      301 cgatccaacc tttatggagc aaatgcgtga tcgagcagta gagttctgag gggataaccg
      361 agctgttatc ccccgtttat tctgccaact ctcaggtctg tcagagttcc aagaatacga
      421 tgcggctggc aaattatgcg gcttttccat taacagattc ggccacaaga acaacaccgt
      481 gtttatttcg atcaggatgt tcactccaga tttctgattc aggaatactt tccccgtcat
      541 agcagatccg atcgccatca aacggataat accgtgcgga atcagtatct aaaataaaca
      601 ctttcagatt cgttgaacga cgcgctatat gctgccagag ggctttacct ccttcatatt
      661 gctcgttatc acttaatagt ataacgcccg cttttgtggc taccgtttca tatacaaacg
      721 tcgcaaggcc aacatcacgt acagccccat caacaacaat cccttcaatc tgagcgacgt
      781 cctgctcttc ccagaatttc cggacaaaca ccccttcaag ctgtgagcga cgctgaagct
      841 ccagagtggc aacaacttta tggcgaatat caccatcaat aacagaaggt gcagcttcga
      901 ttaccgcagc gaacggagtc tttggatccc agatgatctg gtaaagcacg ccctgaatag
      961 ttactccatc cagtatcgaa aaacgacgag ctacagtagc aggggagtaa gacattggaa
     1021 ttacagccat ttttgaatcc cctctgagta atacaggcac gaaaatttac ctcaataatt
     1081 atagtaaaca atacaagaac taggtgcatc aacaataccc atcaattgta cgcaattcgt
     1141 acagtaacca ggaactccct caccttctgc caaacagatt gacaacatta ttgcctgttt
     1201 gctgttgata gttattaacc cgatcctccc agatctcaag tgctctgcgt ttttctggca
     1261 aataatcgta tcgatcataa tgttttgaac tgacatcgtt caacgcatga ttttgaatac
     1321 gatcgcggat ttccttactg attccagctt ctcccattaa cgttttacag gtccgacgta
     1381 agtcacgtgc tgtaaaaatt ttaaagtcag gattacaagc ccggaaatac atgatagcgc
     1441 gagccagact gtccgtacga accggacgct taccattagt cgaaagcggg aaaatgtaag
     1501 ggctcgattt ttcttttgtt aactcctgca cacaggataa ttcctgtaat gctgattcag
     1561 tcaacggtat caaatgctct cgtttatttt tagagacatc cgcagtcacc agcagcgttt
     1621 tttgctgaaa atcaacatca caccactgac tggcgatcat ttcaaaggga cgttgccctc
     1681 cggtatagat acataatcga atcaaatgtt gcatcaataa tccaacgttg gttgcttcag
     1741 caaaatgttc cattagaaaa cgcaactctt cgagtgtcag ccaggtgtca ccgacttttt
     1801 ctgcagatga ctgtttcggt atcactgata caggattggc ctccagccca aaactaatcc
     1861 ctacacttgt gttcatcgga tcgttatcag ctttcagacc atagttaaat gcagccatca
     1921 ggtaagaacg gatccggtta gagtgaacca cagcaccgcg ctgaatgatc ccggccaaga
     1981 tatgtttaat ctgtccggaa gtcacatctt tagctttagt ctcacggggg atcactgtat
     2041 agcattcatt ttcaaggcgt ttcagaacat cctgccatgt tctcttatta tcaattttca
     2101 ttttgttaac ataaccatga atcaatgctt caaatgaacc ctgtgattga tggagttgtc
     2161 tccgctgctg ttctgcaagc tgttgtcgct ccagttctgt ctggggatcc tttccttcaa
     2221 tgagccaggc accatacttt tttgccagtt cgttcgctgt ggccagtttc atctcaggcc
     2281 atatacccaa ttgaatgaat ctttcttttg tccctttttc cacgtaatag cggaaataga
     2341 aaattttgct gccagaagac tgaactttga cgccaagtcg tccagtgcca cgttgagagc
     2401 tgttgctcca gacatagaac gatgtctttt ttgcttttaa tgctcgaata gctgtttcag
     2461 ttagattgac ggccatagaa cctcttttct aaatcagtaa caatcaaaat aactcgtaaa
     2521 tttgggtcag gtaatgggtc aggtaacgat cgtacaatgg agaaaaaacg gaaccaagcg
     2581 gaaacagaag aaattacata attagttgat tataaacaat taataaaaca attacgaaca
     2641 aaccaaaaca atacagaatg gacttgcctc atgactcata atcgcttggt cgctggttca
     2701 agtccagcag gggccaccag atatagcaaa ggctgacgag aaatcgtcag cctttttctt
     2761 tttatatatc agttactttg cgtgccagta agccgctgca cgtacccgct gtgggtcata
     2821 ctgttccgct tcaaagcggc ggcttaaatt cttaacgact ttgccttcgc cggttatcca
     2881 gatgaagtaa tcatcggcag ggatttgcat ctgcgccaga cgcgcatcta ccgcctgctc
     2941 atcatgcgcc agccattcga tattaaaacc atcaaggtgc gcgagataat cctgacaggc
     3001 gttatcccgc acgctaacca gcgcactaac ttgcggttta acggcaagtt tgctcaacgt
     3061 ttccaggcgg cggcgcagtg cgggcattcc ggattcatcg cagacataca gctgatacgc
     3121 gtaatcctct ggcaccacca gcgaaccgcg cggacctgcc accgtgagtt tatcgcccgg
     3181 ttgcgcctgc attgcccagc tgctggcgac gccaccttcg tgaataaaga aatcaatcgc
     3241 cagttcatgg cgtagttcgt catacagcgg cgtatagtca cgcgacggtg ggcgtggtcc
     3301 ttccggccag acgatgccct cttccgttac cgttggcggc acaaagtgag catcagattg
     3361 aggaaagaag agtttgctgt gatcgtcaaa gccgcgcgat gtaaaaccgt ccagcgcctc
     3421 gccgccaagg acaatgcgct gaaaaccgac gctaatacgc tcaacgcgta gcacagtcaa
     3481 ttcacggaag cgcagatcat tgcgaacgcg ctgtgggtag cggggggtgt tattcattgt
     3541 tatcgccttc atgatggtaa tcagatatat ctaaataaaa tttcgcaaat gataatgatt
     3601 gttaatcatg ataaatgcaa gcgatttgta gaactgatat gtctatagtc tgataagacg
     3661 aaccgcctct tctcaggcat cattactcaa cgccggatgc ggcgtgaacg ccttatccgg
     3721 cctacgtgtg agatgagtcc acgtttaaaa atcattattg tacttgcagt aatgctcata
     3781 tcagatataa attagatata tctaattaaa caaaaggagg ctgatatgag ccatcatcac
     3841 gaagggtgct gtaaacatga aggccagcca cgccatgagg gctgctgcaa aggtgagaag
     3901 tcagaacacg agcactgcgg acacggtcac cagcatgaac acggtcaatg ctgcggtggt
     3961 cgccacggtc gcggcggcgg tcgtcgacaa cgtttctttg gtcacggtga attgcgtctg
     4021 gtgattctgg atattctctc gcgcgatgac agccacggtt acgagttgat taaagcgatt
     4081 gaaaatctga cccaggggaa ttacacccca agcccgggcg tcatctaccc aacgctggat
     4141 tttctgcagg agcagtcgct gattaccatt cgcgaagagg aaggtggtaa gaagcagatt
     4201 gcgctgaccg aacaaggcgc gcagtggctg gaagaaaacc gcgaacaggt agagatgatt
     4261 gaagaacgca tcaaagcgcg ttgcgttggc gctgcgctgc gccagaaccc gcaaatgaag
     4321 cgggcgctgg ataattttaa agcggtactg gatttacgcg tcaaccagag cgatatcact
     4381 gatgcacaaa taaaaaagat cattgcggtg atcgaccgcg ccgcttttga tattacgcaa
     4441 ctggattaat caccgattgc cggatgtggc gtgccctacc cccgtaggcc tgataagatg
     4501 cgccagcatc gcatcaggca ttgtgcgcca accgccggat gcggcatatc gattaatgca
     4561 gcaccgtcac cgcgtcttcc agtcggctgg cgcggtgttt caccatcgcc gacacctgcg
     4621 cactctcttc cactaactcg gcgtttttct gggtgatcag gttaagctca tccactgcac
     4681 gggtcaggct ggaaagccca tcggcctgtt ccagcgttga atggctaatc tgagctatca
     4741 actgggtgac gttttttacc tgtgccacaa tatcttccat cgtccgtccg gcggcgtgta
     4801 cctgctgcga accggattgc accttatcag cactggcatc aatcagcttg cgaatatcgt
     4861 tggcagcatt ggcgctgcgg ctagctaaat gacgcacttc ccccgccacc actgcaaaac
     4921 ctttgccctg ttcgccggca cgcgccgctt ccaccgccgc attcagggcc agaatattgg
     4981 tctgaaacgc aatatcgtta atcagcgaag taatggtgcc aatgcgctgg gtactgtcgg
     5041 caatatcgtc cattgtcttg atcaccgtag tcatcgcctc cccaccctgc actgccgcat
     5101 tactggcagt gatagaaagt ttatcggcag ccgacgccgt ggcagagttc tgtttcaccg
     5161 acgccgccat ttggttcatg gtcgccaccg tttgctgaac gttatcaact gtctgctggg
     5221 tatgttcgtt cagttcatcg gtgcctttcg ccagcgtctc actgccgttt ctgacactgg
     5281 acacctggct tgagacatcg ttaattagcc aacggcacat caggccaagc tgccctaccg
     5341 cgcgtaatgt cagccccagc tcatcgctgc gattcagatg ctcaacacta ttacgctctc
     5401 cggtcgccac cttcagtgcc tgacgggcga cattttctat cgggcggaca atctgccatt
     5461 caaaacaagc gcttgccaat aataccacta acgcacagag gatatacgtc accaccgggg
     5521 cagcaacaaa ccatagcatg gccgtcagca agataaacat cagggtcatc acgccacgcg
     5581 cccgccagcg aagcggtaat gaaggcagtt tacccagcca tcctttacgc accaccaggc
     5641 ctttatgaat acgcttactg gtacgtccgg cgttcaacgc tttgtacagc ggctccaccg
     5701 ccgcgatctc ttcatccgtc gcccgggtac gaatcgacat atagccactg atttttccct
     5761 cgcgcaccat cggtaccgca ttggcccgca cccaataatg gtcgccattt ttgcggcgat
     5821 ttttcacgat gccgctccag ggctcccctt ttttcagggt gaaccacata tccgcaaacg
     5881 ccgcttttgg catatccggg tgacgcacca tgttgtgcgg ttgaccttgt aactcttgca
     5941 aggtatagcc gctcacctgc acaaaagtgt cattagcatg agtgatatag ctttgcagat
     6001 cggtagtgga catcagagtg gtatcgtccg ccagcggggt attttgctgg gtgacatacg
     6061 gatgagaaga catgttcgcg tcctgtgcag gttatatggt tgttaacttc ttgtcagagt
     6121 ttatgtcggc cccgctgcgg ttatctttaa ctgattaatt tgatttagat cgcaatttgc
     6181 gattaaagca taaatctaat cccttgattt aaaatacttt cactctgtta ctatacgaaa
     6241 acgttaatta tcttgcccaa aaatcaggca gttgttgccc tgaaagtgtg catttgcgca
     6301 gcaatcgcca aatccatacc cgacaaaaac cgtgcaaaat aacaacagat gttaacaaat
     6361 agcattaaat attgcgcaaa tgataaccac atttgtgttt atcccgattt tcgcgatcgc
     6421 agccggagtg gcgcaatccc tgcaatactt aaatcggtat catgtgatac gcgagcctcc
     6481 ggagcatatt ttgaacaggt taccttcgag cgcatcggct ttagcgtgca gcgcccacgc
     6541 cctgaatctc attgagaagc gaacgctgga tcatgaggag atgaaagcac ttaaccggga
     6601 ggtgattgaa tacttcaaag agcatgtcaa tccggggttt ttagagtatc gcaaatctgt
     6661 taccgctggc ggggattacg gagccgtaga gtggcaagcg gggggtttaa atacgcttgt
     6721 cgacacccag ggacaggagt ttatcgactg cctgggaggt tttggaattt tcaacgtggg
     6781 gcaccgtaat ccagttgtgg tttccgccgt acagaatcaa cttgctaaac aaccgcttca
     6841 cagccaggag ctgctcgatc cgttacgggc gatgctggcg aaaacccttg cggcgctaac
     6901 gcccggtaaa ctgaaataca gcttcttctg taatagcggc accgaatccg ttgaagcggc
     6961 gctgaagctg gcgaaggctt atcagtcacc gcgcggcaag tttactttta ttgccaccag
     7021 cggcgcgttc cacggtaaat cacttggcgc gctgtcggcc acggcgaaat cgaccttccg
     7081 caaacctttt atgccactgc tgccagggtt tcgtcatgta ccgtttggca atatcgaagc
     7141 catgcgcacg gctcttaacg agtgcaaaaa aaccggtgat gatgttgctg cggtgatcct
     7201 cgaaccgatt cagggcgaag gtggcgtcat tctgccgccg ccgggatatc tcaccgccgt
     7261 gcgtaagctg tgcgatgagt tcggcgcact gatgatcctc gatgaagtgc aaaccggtat
     7321 ggggcgcacg ggcaagatgt tcgcctgcga gcatgagaac gtacagccgg acatcctgtg
     7381 tctggcaaaa gccctcggcg gcggcgtgat gccgattggc gcaaccatcg ccactgaaga
     7441 ggtgttttcc gttctgttcg acaacccgtt cctgcatacc accacctttg gcggcaaccc
     7501 gctggcctgt gcggcggcgc tggcgaccat caatgtgttg ctggagcaga acttaccggc
     7561 tcaggctgaa caaaaaggcg atatgttgct ggacggtttt cgtcaactgg cgcgggaata
     7621 tcccgatctg gtacaggaag cgcgtggtaa agggatgttg atggcgattg agtttgttga
     7681 taacgaaatc ggctataact ttgccagcga gatgttccgc cagcgcgtcc tggtggccgg
     7741 aacactcaac aacgctaaaa cgatccgcat tgaaccgcca ctgacactga ccattgaaca
     7801 gtgtgaacag gtgatcaaag cggcgcgtaa ggcgctggcg gccatgcgag tcagtgtcga
     7861 agaagcgtag tatcaaatcg gatggcgata cgacgtcgcc atccgatctt tttatacgat
     7921 acgtacgccc gcaggcatca tccgctccgg cgttaacagc acgctttcac tgccgtcatc
     7981 ggtttccgca cacaacagca tgcattccga cgtttcaccg cgcattttcg ctttttgcaa
     8041 attgcataag accaccactg tttttcccat cagctcttct tcgctgtagt acggcaccag
     8101 gctggtcacg gtttgcagcg ttttttcccc cacatcaacc tgtacgatgt acagcttgtc
     8161 ggcgttttca tggcgtttca cttccacaat ctttccgacg cgcatttcca gacgtgcaaa
     8221 atcagcgtaa gccacggttt ccattgtttc ctcccttcga gtaaaatttt actaaactat
     8281 agcaaaagtt tttctcaatc ctgtaggcta aaaatggaga atgcaggcgt gatcacattc
     8341 gcaagccgct gtgttaccgt tacagcgtca aagaaacgcg ctttatttac tgaaaacagg
     8401 tgacccgata agcacttcct ctacaatggg ggcgcacatc agggaaagta aaaaaggtaa
     8461 acatggcaac actaaaagac atcgcaatcg aagctggcgt atccctggcg acagtatcca
     8521 gggtcttgaa tgacgatccg acattgaatg tgaaagaaga gacgaaacat cgcattctcg
     8581 agatcgccga aaagctggag tacaagacca gtagcgcccg taaactccag acaggcgcag
     8641 tcaaccaaca ccatattctg gctatctaca gctaccaaca ggagctggag atcaacgatc
     8701 cttactatct ggcgatccgc cacggcattg aaactcagtg cgaaaagctg gcgatcgaac
     8761 tgaccaactg ttatgaacac aacggcttac cagacattaa aaacgtcacc ggtattttaa
     8821 ttgtcggcaa acccacgtcc gccctgcgcg ccgccgcctg tgcgttgacc gacaatatct
     8881 gttttatcga ctttcacgaa cccggcagcg gttacgatgc ggtggatatc gatctggcac
     8941 gtatcagtaa agaaatcatc gatttctata tcaatcaggg cgttaatcgt attggtttta
     9001 ttggcggtga agacttgcct ggcaaggcgg atattcgtga ggtcgccttt gcggaatatg
     9061 gtcgtctgaa acacgtggta cgcgaagagg atatctggcg cggcggtttt tccagttcgt
     9121 cgggctatga actggcaaaa cagatgctgg cgcgggaaga ctatccgaag gcactgtttg
     9181 ttgcttccga ttccattgct atcggcgtac tgcgggcaat tcatgaacgt ggcctgaaca
     9241 tcccacagga tatttcgctt atcagcgtta acgatatccc caccgcgcga tttacctttc
     9301 cgccgctctc caccgtgcgc atccattccg agatgatggg aagtcagggc gttaacctgg
     9361 tgtatgaaaa agcccgcgat ggtcgcgcgc tgccgctgtt ggtcttcgtt cccagcaaat
     9421 taaaactgcg cggcacgacc cgttaaatcc ccttgcacgc tgtccggcaa tcgtttttgc
     9481 cggacagtgc tgccgtttat tttcgtgatc cagttaaagt aaatgcattt atccgctact
     9541 ttttagtaaa atttttacta aactccccag caattacaca aactaccatc accataaatg
     9601 gttccgattt ctctctaccg ggaggcccta tgaatcgctg ggaaaacatt cagctcaccc
     9661 acgaaaaccg acttgcgccg cgtgcgtact ttttttcata tgattctgtt gcgcaggctc
     9721 gtacttttgc ccgtgaaacc agcagcctgt ttctgccctt aagcggtcag tggaatttcc
     9781 acttttttga ccatccgctg caagtgccag aagccttcac ctctgagtta atggctgact
     9841 gggggcatat taccgtcccc gccatgtggc aaatggaagg tcacggcaaa ctgcaatata
     9901 ccgacgaagg ttttccgttc cccatcgatg tgccgtttgt ccccagcgat aacccaaccg
     9961 gtgcctatca acgcattttc accctcagcg acggctggca gggtaaacag acgctgatca
    10021 aatttgacgg tgtcgaaacc tattttgaag tctacgttaa cggtcagtat gtggggttca
    10081 gcaaaggcag tcgcctgacc gcagagtttg acatcagcgc gatggttaaa accggcgaca
    10141 acctgttgtg tgtgcgcgtg atgcagtggg cggactctac ctacgtggaa gaccaggata
    10201 tgtggtggtc ggcggggatt ttccgcgatg tttatctgat cggaaaacaa ctcacgcata
    10261 ttaacgattt caccgttcgt accgactttg acgaagccta ttgcgatgcc acactttcct
    10321 gcgaagtggt gctggaaaat ctcgccgcct cccctgtcgt cacgacgctg gaatataccc
    10381 tgtttgatgg cgaacacgtg gtgcacagca gcaccattga tcatttggca attgaaaaac
    10441 tgaccagcgc cagttttgct tttactgtcg aacagccgca gcaatggtca gcagaatccc
    10501 cttatcttta ccatctggtc atgacgctga aagacgccga cggcaacgtt ctggaagtgg
    10561 tgccacaacg agttggcttc cgtgatatca aagtgcgcga cggtctgttc tggatcaata
    10621 accgttatgt gatgctgcac ggcgtcaacc gccacgacaa cgatcatcgc aaaggccacg
    10681 ccgttgggat ggatcgcgtc gagaaagatc tccagttgat gaagcagcac aacatcaact
    10741 ccgtgcgtac cgctcactac ccgaacgatc cacgttttta cgaactgtgt gatatctacg
    10801 gcctgtttgt gatggcggaa accgacgtcg aatcgcacgg ctttgctaat gtcggcgata
    10861 tcagccgtat taccgacgat ccgcagtggg aaaaagttta cgtcgagcgc attgttcgcc
    10921 atattcacgc gcagaaaaac catccgtcga tcatcatctg gtcgctgggc aatgaatccg
    10981 gctatggctg taacatccgc gcgatgtacc atgcggcgaa ggcgctggat gacacgcgac
    11041 tggtgcatta cgaagaagat cgcgatgctg aagtggtcga tattatttcc accatgtaca
    11101 cccgcgtgcc gctgatgaat gagtttggtg aatacccgca tccgaagccg cgcatcatct
    11161 gtgaatatgc tcatgcgatg gggaacggac cgggcgggct gacggagtac cagaacgtct
    11221 tctataagca tgattgcatt cagggacatt atgtctggga atggtgcgac cacgggatcc
    11281 aggcgcagga tgacaacggc aatgtctggt ataaattcgg cggcgactac ggcgactatc
    11341 ccaacaacta taacttctgt cttgatggtt tgatctattc cgatcagacg ccgggaccgg
    11401 gcctgaaaga gtacaaacag gttatcgcgc cagtaaaaat ccacgcgctg gatctgactc
    11461 gcggcgagtt gaaagtcgaa aataatctgt ggtttaccac gcttgatgac tacaccctgc
    11521 acgcagaggt gcgcgccgaa ggtgaaacgc tcgcgacgca acagattaaa ctgcgcgacg
    11581 ttgcgccgaa cagcgaagcc cccttacaga tcacgctgcc acagctggac gcccgcgaag
    11641 cgtttctcaa cattacggtg accaaagatt cccgcacccg ctacagcgaa gccgggcatt
    11701 ctatcgccac ttatcagttc ccgctgaagg aaaacaccgc gcagcctgta cctttcgcac
    11761 cgaataacgc ccgtccgctg acgctggaag acgatcgttt gagctgtact gttcgcggct
    11821 acaactttgc gatcaccttc tcaaaaacga gtggtaaacc gacatcctgg caggtgaatg
    11881 gcgagtcgct gctaacccgc gagccaaaaa tcaacttctt caagccaatg atcgacaacc
    11941 acaagcagga gtacgaaggg ctgtggcagc cgaatcattt gcagatcatg caggaacatc
    12001 tgcgcgactt tgccgtagaa cagagcgatg gtgaagtgtt gatcatcagc cgcacggtta
    12061 ttgccccacc ggtgtttgac ttcgggatgc gctgcaccta catctggcgc atcgctgccg
    12121 atggccaggt taacgtggcg ctttccggcg agcgttacgg cgactatccg cacatcattc
    12181 cgtgcatcgg tttcaccatg gggattaacg gcgaatacga ccaggtggcg tattacggtc
    12241 gtggaccggg cgaaaactac gccgacagcc agcaggctaa catcatcgat atctggcgca
    12301 gcaccgtcga tgccatgttc gagaactatc ccttcccaca gaacaacggc aaccgtcagc
    12361 atgtccgctg gacggcactg actaaccgcc acggcaatgg tctgctggtt gttccgcagc
    12421 gccccattaa cttcagcgcc tggcgctata cccaggaaaa catccacgct gcccagcact
    12481 gtaacgagtt gcagcgcagt gatgacatca ccctgaatct cgaccaccag ctgcttggcc
    12541 tcggctccaa ctcctggggc agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact
    12601 tcagctacgg ctttacgttg ctgccggttt ctggcggaga agctaccgcg caaagcctgg
    12661 cgtcgtatga gttcggcgca gggttctttt ccacgaattt gcacagcgag aataagcaat
    12721 gaggatcatc gataacttag aacagttccg ccagatttac gcctctggca agaagtggca
    12781 acgctgcgtt gaagcgattg aaaatatcga caacattcag cctggcgtcg cccactccat
    12841 cggtgactca ttgacttacc gcgtggagac ggactccgcg accgatgcgc tatttaccgg
    12901 gcatcgacgc tattttgaag tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc
    12961 gccgaaagag acattacagg tcgtggaata ttatcgtgat gaaactgacc gtgaatattt
    13021 aaaaggctgc ggagaaaccg tagaggtcca cgaagggcaa atcgttattt acgatatcca
    13081 tgaagcatat cggtttatct gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat
    13141 cgaagatggt tatttccata acaaataaca actacggcgg caaaaggagt ttgccgccac
    13201 cgctacccta ctcattttcg gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc
    13261 aaattcggct tgctctcgct gacttttgcc gccgtttaca gctttaacaa cgttatcaat
    13321 aataatattg agcttggact ggcctcggca ccgatgtttt tcctcgcgac gattttttat
    13381 tttattccct tctgtctgat catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc
    13441 ggtgtctacg cgtgggtaaa aagttcgctg ggcgggcgtt gggcatttat tactgcctat
    13501 acctactggt tcgtaaacct gttctttttc acctcactgt tgccgcgcgt tattgcttat
    13561 gcttcgtatg ccttcctcgg ctacgaatat attatgacgc cggttgccac caccattatc
    13621 agtatggtgc tgttcgcctt ctccacctgg gtttccacca acggggcgaa aatgctgggg
    13681 ccaattacct ccgtcacttc aacgctgatg ctgctgttaa cgctctccta cattttactg
    13741 gcaggtacgg cactggttgg cggcgtacag cctgccgatc cgatcaccgt tgacgcgatg
    13801 atcccgaact tcaactgggc gttcctcggc gttaccacct ggatctttat ggccgcaggt
    13861 ggcgcggagt ccgtcgcggt gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt
    13921 aaagtgatca tcctcgccgg gatttttatc ggcgtactgt attccgtttc ctcggtgctg
    13981 attaacgtct tcgtcagcag caaagagttg aaatttactg gcggatcggt gcaggtattc
    14041 cacggcatgg cggcgtattt tggtctgccg gaagcgttga tgaatcgctt tgtcggtctg
    14101 gtgtccttta ccgcgatgtt cggttccctg ctgatgtgga ccgcaacgcc ggtgaaaatt
    14161 ttcttctccg aaatcccgga aggcatcttt ggtaagaaaa ccgtcgaact gaacgaaaac
    14221 ggcgttccgg cgcgcgcagc gtggatccag ttcctgatcg tcatcccgct gatgattatc
    14281 ccgatgctcg gttccaatac cgtgcaagat ctgatgaata ccattattaa tatgaccgcc
    14341 gcagcgtcca tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa
    14401 ttagatcacc tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt
    14461 tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg
    14521 aatattctga ccatcatttt ttataacgtc ggcggtattg ttatcttcct cggctttgcg
    14581 tggtggaaat ac
//
LOCUS       DAZKJR010000127        14310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKJR010000127 DAZKJR010000000
VERSION     DAZKJR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.128"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..598
                     /locus_tag="ACHOWS_003949"
     CDS             32..598
                     /locus_tag="ACHOWS_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0049452.1"
                     /translation="MFKGQKTLAALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            649..3108
                     /gene="ybgQ"
                     /locus_tag="ACHOWS_003950"
     CDS             649..3108
                     /gene="ybgQ"
                     /locus_tag="ACHOWS_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFQ0049453.1"
                     /translation="MDTVNIYRLSFISCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKGKGVIVPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRANNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATDSTISLA
                     AYRFSDRHFHSYANYLDHKYNNSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDNSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSLHLPEHMEDTANRLILPCH"
     gene            3123..3854
                     /gene="ybgP"
                     /locus_tag="ACHOWS_003951"
     CDS             3123..3854
                     /gene="ybgP"
                     /locus_tag="ACHOWS_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFQ0049454.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            3851..4912
                     /gene="ybgO"
                     /locus_tag="ACHOWS_003952"
     CDS             3851..4912
                     /gene="ybgO"
                     /locus_tag="ACHOWS_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0049455.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREIPPHDYQSSLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            5065..6111
                     /gene="abrB"
                     /locus_tag="ACHOWS_003953"
     CDS             5065..6111
                     /gene="abrB"
                     /locus_tag="ACHOWS_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0049456.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATNPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6108..6899)
                     /gene="nei"
                     /locus_tag="ACHOWS_003954"
     CDS             complement(6108..6899)
                     /gene="nei"
                     /locus_tag="ACHOWS_003954"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFQ0049457.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTMRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLITHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6935..7669)
                     /gene="pxpA"
                     /locus_tag="ACHOWS_003955"
     CDS             complement(6935..7669)
                     /gene="pxpA"
                     /locus_tag="ACHOWS_003955"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFQ0049458.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSVMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7659..8591)
                     /gene="pxpC"
                     /locus_tag="ACHOWS_003956"
     CDS             complement(7659..8591)
                     /gene="pxpC"
                     /locus_tag="ACHOWS_003956"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFQ0049459.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8585..9241)
                     /gene="pxpB"
                     /locus_tag="ACHOWS_003957"
     CDS             complement(8585..9241)
                     /gene="pxpB"
                     /locus_tag="ACHOWS_003957"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFQ0049460.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGIYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9264..10007)
                     /gene="ybgI"
                     /locus_tag="ACHOWS_003958"
     CDS             complement(9264..10007)
                     /gene="ybgI"
                     /locus_tag="ACHOWS_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFQ0049461.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10278..11759
                     /gene="dtpD"
                     /locus_tag="ACHOWS_003959"
     CDS             10278..11759
                     /gene="dtpD"
                     /locus_tag="ACHOWS_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFQ0049462.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFSHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDIFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11802..13220)
                     /gene="phrB"
                     /locus_tag="ACHOWS_003960"
     CDS             complement(11802..13220)
                     /gene="phrB"
                     /locus_tag="ACHOWS_003960"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFQ0049463.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSASVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERALRNVVCEGFDDGVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSIWLNEL
                     IWREFYRHLITYYPSLCKHRPFIVWTDRVQWQSNSAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13217..13726)
                     /gene="ybgA"
                     /locus_tag="ACHOWS_003961"
     CDS             complement(13217..13726)
                     /gene="ybgA"
                     /locus_tag="ACHOWS_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFQ0049464.1"
                     /translation="MNQQRFDESTLIRIFALYELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRGGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13868..14310)
                     /locus_tag="ACHOWS_003962"
                     /pseudo
     CDS             complement(13868..14310)
                     /locus_tag="ACHOWS_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3434 a         3824 c         3489 g         3563 t
ORIGIN      
        1 cactctatct cattatttaa aggactcagc tatgtttaaa ggacaaaaaa cattggccgc
       61 actggccata tctctgctat tcacagcacc tgtttatgcg gctgatgaag gatccggtga
      121 aattcacttt aaaggtgaag ttattgaagc accgtgtgaa atacatcagg atgatattga
      181 taaagaggtt gaactcggtc aggtgaccac cagccacatt aatcagtcgc atcacagcga
      241 tgccgttgct gtcgacctgc gcttagtcaa ctgcgatctg gaaaactcca acaacggttc
      301 cggcggcaag atttctaaag ttgcagtcac ctttgacagc tcagcgaaaa ccaccggcgc
      361 agatccgatt ctcaacaaca ccagcacggg tgaagcgacc ggtgtcggcg tgcgtttaat
      421 gaataaagat caaagcaata tcgttttagg caccgctact ccagatatcg acctggcacc
      481 aacctccagc gaacaaacgt taaatttctt tgcgtggatg gaacaaattg atcaggctac
      541 gcccgtaaca ccaggcgcag ttacggcgaa tgcgacctac gtactggatt ataaataagt
      601 atcactttgg cggcgtcatc gtcgcctttc ttcaaatatt tatcaggtat ggacaccgtg
      661 aatatttatc gactctcttt tatatcctgt ctggtcatgg cgatgccttg cgcattggcg
      721 gtcgaattca acctgaatgt cctcgataaa tcgatgcgag atcgcattga tatttcatta
      781 ttaaagggaa aaggggtcat tgttcccggt gaatattttg ttagcgttgc agtaaataac
      841 aaccaaatca gtaacggaca aaaaattgac tggaaaaaga acggtgacca aaccattccg
      901 tgtattaatg atttactggt agataaattt ggtttaaaac ctgaagttcg tcagtcgtta
      961 ccacggttaa accagtgcgt cgattttagc tcccggcctg aaattctctt catttttgat
     1021 caggctagcc agcaactgaa tatcactatt ccacaagcct ggctggcgtg gcactcagat
     1081 aactggactc ccccctcaac atggaaagaa ggtgtcgccg gcgtcctgat ggactacaac
     1141 ctgtttgcaa gcagctaccg cccacaggat ggtagcaaca gcactaacct gaacgcctat
     1201 ggaacagccg gaattaacgc gggagcatgg cgtttacgca gtgattacca attgaatcag
     1261 accgatagcg atgataacca tgaacagtca ggcgaaatat cacgcaccta tctttttcgt
     1321 ccattaccgc aattaggttc aaaattaacc cttggtgaaa cggatttcag ttccaatatt
     1381 ttcgacggct tttcttatac cggcgcggca ctggcaagtg acgatcggat gttaccgtgg
     1441 gagctacgcg gctacgcccc acaaatcagc ggcattgcac aaaccaacgc caccgtcacc
     1501 gtgagtcact cagggcgagt tatttaccag aagaaagtcc caccaggtcc gtttatcatt
     1561 gatgacctta atcagtccgt ccagggtacg ttagatgtca aagtgacgga agaagatggt
     1621 cgggcgaaca atttccaggt ttcggcagca tcgacgcctt tccttacgcg ccaggggcag
     1681 gttcgctata aacttgctgc cggtcagccg cgaacatcta tgtctcatca aactgaaaat
     1741 gaaacctttt ttagcaatga agtttcctgg gggatgctgt caaatacctc actgtacggc
     1801 ggcctgctga tttcgggtga cgactaccat tctgccgcaa tgggtatcgg gcaaaatatg
     1861 ctgtggcttg gtgcattgtc gtttgatgtc acgtgggcca gtagtcagtt tgatactcag
     1921 caggacgagc ggggcttaag ttaccgcttt aattacagca aacaagtgga tgccaccgac
     1981 agcacgattt cgctcgccgc ttatcgtttc tccgatcgtc attttcacag ctacgccaac
     2041 tatctggatc acaaatacaa caacagcgat gcgcaggacg aaaaacagac gatcagctta
     2101 tccgtgggcc aaccgattac cccactgaac ctcaatctgt acgccaacct gctgcatcaa
     2161 acctggtgga atgcagacgc ctccacgacc gccaacatca cggcgggttt taatgttgat
     2221 attggtaact ggagagatat ctcgatctcg acgtcattca atacaaccca ttacgaagat
     2281 aaagatcgcg acaatcagat ttacctgtcg atttcgctcc ccttcggtaa cggggggcgg
     2341 gtcggttatg acatgcaaaa cagtagtcac agcaccacgc accgcatgtc gtggaatgat
     2401 acgttagatg aacgtaatag ctggggcatg tctaccggac tgcaatccga ccgtccggat
     2461 aatggagccc aggtgagcgg taactatcag cacctgagtt cagcgggtga gtgggatatt
     2521 tctggtacct atgccgccaa tgattacagt tccgtcagca gcagctggag cggttctttc
     2581 acggcaactc aatatggtgc ggcgttccat cgtcgcagct ccaccaatga accacgtctg
     2641 atggtcagta ccgatggcgt ggcagatatt ccggttcagg gcaatctcga ctacaccaac
     2701 cattttggca ttgcggtggt gccgttgatt tccagttatc agccttccac cgtggcggtg
     2761 aacatgaatg acttacccga cggcgtaaca gtggcagaaa acgttatcaa agaaacgtgg
     2821 attgaaggcg cgataggtta caaatcgctg gcttcccgtt ccggtaaaga cgttaacgtc
     2881 atcattcgca acgccagcgg tcagttccct cccctcggtg cggatatccg ccaggataac
     2941 agcggcatta gcgtggggat ggttggcgag gaaggacatg cctggttaag cggagtcgct
     3001 gaaaatcaac aatttaccgt gatctggggt gatagccagc gttgctcgct ccatcttcct
     3061 gaacatatgg aagacaccgc aaatcgcctg attttacctt gtcattaatt aaggaaatga
     3121 aaatgacatt tatgaaagga ctaccccttt tgttgttggt tgccagtctg tgcagccacg
     3181 ctgcactaca acccgatcgc actcgtattg tgtttaacgc taatgataaa gctaccagtc
     3241 tgcgcgtgga taatcgtagc gataaactcc cctacctcgc ttattcctgg ctcgaaaatg
     3301 aaaaagggga gaaaagcgat gatcttctgg ttgccctgcc accgatacaa cgtctggaac
     3361 cgaaagcaac aacgcaggta cggatagtaa agcaagcctc gaccacaaaa ttaccgggcg
     3421 atcgcgaaac gctatttttc tacaatatgc gtgaaattcc tccctcccca gaaaaaaaca
     3481 gcggccatgc cgttctgcaa gtcgccattc agagtcgtat caaggtattc tggcgaccgg
     3541 ctgcattacg caagaaagca ggagaaaagg tcgaactgca gttacaggtc agccagcagg
     3601 gcaaccaact gacactgaaa aatcctaccg cgtattatct gaccattgcc tatctgggga
     3661 gaaatgaaaa aggcgttctt cccggcttca aaaccgtgat ggttgcgcct tttagtacag
     3721 taattacaaa caccggaagt tatagcggca gtcagtttta tctcggttat atggatgatt
     3781 acggcgcgtt acgcatgacg acgctcaact gcagcggaca atgccgttta caagcagtgg
     3841 aggcgaagaa atgagtgctg gcaagggagt gttgctcgtc atctgtctgg tatttctgcc
     3901 attgaagtct gcgctagcgc tgaactgcta ttttggtact tccggcggaa cggtagaaaa
     3961 atcggaagcg attcaaccct ttgctgtgcc aggtaatgcc aaacccggcg ataagatctg
     4021 ggaatctgac gatattaaaa ttcccgtcta ttgtgacaac aataccaacg gcaactttga
     4081 aagcgaacat gtctacgcat gggtaaaccc ctacccgggg gtacaggatc gttattatca
     4141 actcggcgtg acatacaatg gtgtcgatta cgatgccagt ctggggaaaa gccgcatcga
     4201 caccaaccag tgtatcgaca gtaagaacat taacatttat acccctgagc agattatcgc
     4261 gatgggctgg cagaacaaaa tatgctcggg tgaccccgct aatattcaca tgtcgcgaac
     4321 ctttcttgct cgtatgcggt tatacgttaa gattcgcgaa ataccgccgc atgattatca
     4381 aagttcgctt agcgactaca tcgtcgtgca atttgatggt gccggtagcg ttaacgaaga
     4441 ccctaccgcc caaaacctga aataccatat tactggtctg gaaaacattc gtgtactgga
     4501 ttgcagcgtc aatttttcta tttcaccaga aacacaggtg attgattttg gtaaatttaa
     4561 cctgctggat attcgccgcc acacaatgtc gaaaacattc agtattaaga cgacaaaatc
     4621 acaaaatgac cagtgcaccg acggatttaa ggtcagctct tcgttttata ccgaagaaac
     4681 attgattgaa gaggataaag cactgctgat tggtaatgga ctgaagctac gtttgctgga
     4741 tgaaaattct tcaccctata ctttcaacaa atacagcgaa tatgctgatt tcaccagcga
     4801 tatgctgatc tacgaaaaaa cctatacggc tgaactttcg tccattcccg gcacccccat
     4861 cgaagcgggt cccttcgata cagtggtgct ttttaagatt aactataact aatttatcat
     4921 gatgcccttt gcagttagta aagggcctct ctttcagaaa attgctgatt ttcccctttt
     4981 ccttgattgc tgttattttt tcctggcccg gctttcttta tgctgtatcg atacaccaaa
     5041 gaaaaaataa tgagggaacg aaggatgcca gttttgcagt ggggaatgtt atgtgtgctc
     5101 tcacttctcc tttcaattgg tttcctcgcg gttcatctcc cggcggcgct attacttggg
     5161 ccgatgatcg ccgggatcat ctttagtatg cgcggaatta ctctgcaact tccccgctcc
     5221 gcttttctcg ccgcccaggc cattcttggc tgcatgattg cgcaaaacct gaccggttcg
     5281 atcctgacca cgctagcggt gaactggccg attgtgttag ctattttact ggtgacgtta
     5341 ctttccagcg ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc
     5401 ggtgcctggg gttcttctcc tggcggcgcg gcggcaatgg tcgctatggc gcaagattac
     5461 ggcgcagata ttcgcctggt ggcgtttatg caatatctgc gagtgctgtt cgtcgcaggg
     5521 gccgctgttc tggtcacccg catgatgctg ggcgataacg ctgaagcggt taaccagcag
     5581 atcgtctggt ttccgccagt gagcattaat ctcctgctta cgattttgct cgcggtcgtt
     5641 gccggtacag tgggatgctt gctacgtctt ccttccggca cgatgctcat cccgatgctg
     5701 gcgggcgcag tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctt
     5761 gcgatggcgt atatggcaat tggttggcgg attggtcttg gtttcgataa acaaatctta
     5821 ctgcgggcat tgcgcccgct accgcaaatc ctgctgtcga tttttgctct gctggctatt
     5881 tgtgcgggta tggcgtgggg gctgacccgg tttatgcata ttgattttat gaccgcctac
     5941 ctcgccacca accccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc
     6001 gatatggcgc tcatcatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacggga
     6061 cctgccattg cgcggtttat ttcaacctat gcgccgaagc gttctgccta atgctggcag
     6121 ccagggcacc agtaaaacgg gcgagatgac agcgtggttt tctcaatgat gccgccacag
     6181 cgttcgcaca gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc
     6241 ttattctcat ccacctgccc ccgcgtagcg taggaaaatc gaggaatatc cagtaacgcg
     6301 tgtgcgagtg catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac
     6361 ccaacctgcc agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga
     6421 tcgagcagta atccagcaaa ctgacggtta cgaaagcgcg gcgacaataa ccgttccttt
     6481 accacctccg gcgtcagatt cggatccagc acatcggggc cgactcgttg taaaaacgga
     6541 tgcgtgatca gttgttccgg cgtcaacatc tcaatatccg aggcgctata aagcagaata
     6601 gttttgtcaa ccgtttgcag ttttacccgc aatactcgca tagtctgcgg ctcttcgccg
     6661 gtatcaacca cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taagtcgttg
     6721 gaaaaatgag ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata
     6781 agttgtgatt gataaggttt taactgcggg aaggcaaacc agacatcagt tagtggtttg
     6841 cctttgatcg ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg
     6901 gtgatatcct tttttcattc tttattaaac ggggttatgc tgcgacaaca atcccctttt
     6961 cggcaaatgt agagcgtagt cggcgggcga atgccagtgc gtgctcgccg tcgccatgca
     7021 ggcagaccgt ttgcgccgtg accgttgccc attcgccggt gatgcttttg actctgccgt
     7081 gttgcaccat ctccagcgtt tgcgccagcg cctgttcttc gttttcaatc aacgcgcctg
     7141 gctggcttcg cggcaccagc gaaccgtctg cctgataacc gcgatcggca aacacttcct
     7201 cgcgcgttgt caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccga
     7261 cgagaatcaa tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg
     7321 cctctttcgc tgcctggttg tataacatgc cgtgaggttt gacatgacgc ataacaccgc
     7381 cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg
     7441 tttctggcgg cagctgcatg acgctgcgcc caaaattttc cctgtcggga aaactcgggt
     7501 gagcgccaat cgcaacacca ttttttattg cttcacgcac gcaagcctgc atggtttgcg
     7561 catcgcctgc atgaaatcca caggcaatat tggcagagga aaccagcgtt aatagctctg
     7621 cgtcgctggc atagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc
     7681 cacgctaatt gttcgaaata acgttgctga tcttgccgtg ctttcagtgc ttcttccagt
     7741 gaacactgga caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca
     7801 gcctcaatga tacaggcaat acgcggataa ccaccggtgg tctgtgcgtc gttcatcaac
     7861 acaatcggct gcccgttatg cggcacctgc accacgcccg gtaacaaacc atgagataac
     7921 agttcgcgat cggtggtacg ttttaaaatt tgcccctgta agcgatagcc cattcggtta
     7981 ctttgcgagc taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac
     8041 tcatgatatt ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg
     8101 ccctgcgctt ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc accatccttc
     8161 agtaaacggc cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt
     8221 accggcggaa catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg
     8281 cgtttaagcg ttaaacgctg acctgctttc atcggcaagc gccagccggt ccagacggcg
     8341 ttatcatcca gccgcgcttc gcaaccggcc cccgtcagag caaaccaccc atcagtttcg
     8401 aactcaaccg ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc
     8461 accagtaggt tagcaatgcg cagcgcaggc atatccagtg cgccgcagtg gctgataccc
     8521 gactggcgaa aaccgtgacg accgccatcc tgcacagtgg tatacatacc cgcacgaata
     8581 atcttcagca tactccctcc ttctgcggta caaagcgcac gctgtctccc ggacgtaata
     8641 agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc
     8701 agccacccgg cgttgccaac ggataaatac cagtctgcgg cccgccgatc ccgacagaac
     8761 ctgccggaac gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac
     8821 tcccgagata cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg
     8881 agtgcaattc aacaacctgt ttttcgctca atccacaatg cgccgcgacc accgccaaat
     8941 ccggtccggc tgcgccaccg taaaccaccg gaatttcaat aaagcgagac tccggctcca
     9001 gcgcctcact ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact
     9061 caggattacg caaaatcact gtgatattgt tcatgccagg aatggcttca accacattcg
     9121 gcatatccac cagacgctgt gccagtcgcc agatccgttt ctggctagcc agcgtcaccg
     9181 gcggttccag ctccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca
     9241 ctttcgatcc ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca
     9301 agatcggtat tttcattcag ccactcgctc aatgcgcgaa taccaccgcg ttcagtggca
     9361 tggtgacccg cagcataaaa atgcaatccc tgctcgcggg ctgaatgaat ggtctgttct
     9421 gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactc
     9481 tgcccgccgc ccgtgcacca ggcgacgcgc tgaaccacct caggtccggt atcgccacac
     9541 cataacggct tacgtcccag acgcgcttca atccaggaag ccagctccag tcccggcaca
     9601 ggcatggtca gttcgcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc
     9661 agtaacgccg ccagttgtgc gttattaccc agctccggat gcgcgtccag gggcagatgc
     9721 cagccataca ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg
     9781 cgaatgaccg gagattcacc tttccagaag tagccatgat gcacaatgac cgcatccgcg
     9841 cccagacgca ccgcttcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt
     9901 tgcaccgtct ctttgccttc cacctgcaaa ccattcggcg cgtaatcact aatcgccgcg
     9961 ctgttcagtt tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc
    10021 gtattctgat agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc
    10081 ttttgacata ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg
    10141 cttcagctaa tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta
    10201 tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgcag
    10261 tcaattgtgg aattattatg aataaacacg catcacaacc gcgcgctatt tactatgtcg
    10321 ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg ctgatcctct
    10381 atctcaccaa tcaactaaaa tatagcgata atcacgccta cgagttattt agcgcctact
    10441 gttcgctggt atatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca
    10501 atcgcatggc ggtgatgctg ggtgcgttgt tgatggcgat cggtcatgtg gtgctgggtg
    10561 ccagtgagat ccatccgtca ttcctctatc tgtccctggc gattatcgtt tgtggctatg
    10621 gtctgtttaa atccaacgtc agttgcctgc tcggcgagct gtatgagcca accgatccgc
    10681 gtcgtgatgg cggtttctcg ctgatgtatg cggcgggtaa cgtggggtct attatcgcac
    10741 ctatcgcctg tggctacgcc caggaagagt acagttgggc gatgggcttt ggcctggcgg
    10801 cggttggcat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttctctcata
    10861 cccgcggcgt taacaaaaaa gtactgcgtg cgacaaactt tctcctgccc aactggggat
    10921 ggctgctggt tctgctggtg gcaacacctg cgctgatcac cgtactgttc tggaaagagt
    10981 ggtcggtata cgccttgatt gtcgcgacta ttattggcct gggtgtattg gcaaaaattt
    11041 atcgcaaagc agaaaaccag aaacagcgta aggagctggg gctgattgtt accctcacct
    11101 tcttcagcat gttgttctgg gcattcgcac aacagggggg tagctcgatt agcctttata
    11161 tcgaccgctt cgttaaccgc gatatatttg gttataccgt tccgactgct atgttccagt
    11221 cgattaatgc cttcgcagtt atgctgtgcg gtgtgttcct ggcgtgggtg gttaaagaaa
    11281 gcgtcgcagg taatcgtacc gtgcgcatct gggggaaatt tgctcttggt ctcggcctga
    11341 tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg gtctgcaatg tatggtcact
    11401 cttctctgcc actgatggtg ttaggcctgg cggtgatggg gtttgcggaa ctgtttatcg
    11461 acccggttgc tatgtcgcag attacgcgca ttgaaatccc cggcgtgacc ggcgtattaa
    11521 ccggcattta tatgctgctt tccggcgcga ttgcgaacta tctggccggc gtgattgccg
    11581 atcagacatc gcaggcttcg tttgatgctt ccggagcgat caactactcc atcaatgcat
    11641 atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtgctga
    11701 tgatttggct gtatcaggcg cttaagttca gaaaccgcgc gctggcgctg gaatcttaac
    11761 aggccagcac cgatgcctga taagacgctc gctgcgtttt atcaggctcc tttcctcgct
    11821 tcttcgtacg ctgccagcgt tcgcagcctt gcctctttgt gatcaactat cggttgcgga
    11881 taatccagcg tcacacctgc tttctgcgcc cacttccacg gctcatgcac cactttccct
    11941 ggcacatcgc gcagttccgg tagccactgg cggataaact cgccctcacg gtcaaatttc
    12001 tcgccctggg ttgtcgggtt aaaaatacga aaatacggcg ctgcatcggt tccggttgaa
    12061 gcggcccact gccagccacc gttattggct gccaaatcac catcaatcag ctgcgacatg
    12121 aaatatcgct caccttcgcg ccagtcgatc aataaatctt tcaccaaaaa actggctgta
    12181 atcatccgta gccggttatg catccagccg gtgctgttaa gctgacgcat ggcagcatca
    12241 acaatcgggt atcccgtttt gccttcctgc caggccttta aatgcgcgga attgctctgc
    12301 cactgcacgc ggtcggtcca gacaataaac ggacgatgtt tacacagcga ggggtaatac
    12361 gttatcagat gacggtaaaa ctcgcgccag atcagctcat taagccagat actaccggcc
    12421 ccaccgtcca gcgcctgcgg ctgttccgcc aacaggcgat gcaaacactg gcgaggcgat
    12481 aacccgcccg ttgccaggct ggcggacaaa cggctggtgc cttctaccgc agggaaatct
    12541 cgctgttgct catactctcc ggcaccgttc tggcaaaact ggcgtaattg cgcaatcgcc
    12601 gctttctctt ccaccgggaa atgcgcagta tcgaaaggct gacgaggata attcagcgta
    12661 atggatggcg cgggatctat cgatccgcta ctacgtactt ttggcgcagc gacgcactcc
    12721 ggcatccctt ctcgcagccg tttaagccag gcgtttttaa atggcgtaaa gactttgtac
    12781 atctcatgat tgccagtcat caccgccccc ggcggcagga tcacgccgtc gtcaaatcct
    12841 tcacacacca cgttacgcag cgccctctca gcctgcacat cccgcgcccg ctcattcacc
    12901 tcatactgat agttataaaa cagatgggta acacgatttt ccgcgcacac ctgtttaact
    12961 atttcgacgc tggcggcaaa gtcatccact tcatgaaaca gcaaaggaat gcctttttcc
    13021 gccagcgcga tttgtagccc gttcagttgt gcattgataa attcagcctg acgcggcgac
    13081 atattatgcg ccgcccactg gcgtggtgta gcgatataca acgccagcac gcttgcagac
    13141 gaattgcggc aggcggcagc cagcgcgaga ttatcatgct gacgtaaatc ctggcgaaac
    13201 cagaccagat gggtagtcat aaaactcctg aatggcgcaa gttaatcaca cgcggccaaa
    13261 gttcgaaata acgctgccct gaaagccagg cgtcaggata aagcgccata tagtgtttga
    13321 tacgcatcag cggcgcaaga agcggttgct cgccaccgcg ataactgtcg ataagcgccg
    13381 ccagctgctg gcgttctgtg gaatcaatat gagggcgaaa ataaccctga acgtgcatca
    13441 aaacattggt gtggtcacgt cggttggcgg ggtgagaaag caaaacaact acgcgttggc
    13501 ggtactggtt gtaatagtca tccagatttt gccactggtg aatatcagcc acgaacgggc
    13561 caagtttgcg gtactcgggc tgggaatggg ccagaaagac gagtttatag cggctgtgat
    13621 aatcgttgag tgccccgcgc gttaagccat gttctttcag tcgatgtaac tcataaagcg
    13681 cgaagatacg gattagagtg ctttcatcaa atcgttgctg attcattact gctcctgaga
    13741 tattaacagg agtaagtgta gacgttggca aatgatgttt gtgaagttaa tcctgttgta
    13801 gcagggcaaa attacgaaag cacgctcccc gcaaggactg acgccagata ttttctgtcc
    13861 atggctgccc tttccattca aacgtgaaat caataagttc atcagggaca tcgcaaacaa
    13921 tatgaagacg gatttcttct ctgccgtgac tcttttcact cattgcgtaa ctgccatgct
    13981 ctggattatt taattctttc agcggaaatt tttcctcgaa ggctttattt agccgcccct
    14041 ggtttccttt tacagcgaat aaataatcac cgccctgttt ttgtatcttc tctgcaatat
    14101 ctttctggca acccatcgca tcagttgtga tgatttttcc tttaatattc agcatgttaa
    14161 gaagttccgg gatagctgtg atctcattgg atttctcatc cgtcttgatc tgtccgatga
    14221 ccagactgtg cattgttgag aacgcactaa tgacatgaat cgctcccttg cggcgactct
    14281 tgtcataaga gtgccggagc gtttttccat
//
LOCUS       DAZKJR010000128        14019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKJR010000128 DAZKJR010000000
VERSION     DAZKJR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.129"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            230..1120
                     /locus_tag="ACHOWS_003963"
     CDS             230..1120
                     /locus_tag="ACHOWS_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049465.1"
                     /translation="MPPHKIRQEWLTLDEAAEVIRQHNITLLPADLIRHALHGDLSLS
                     VYFPSPVLLRPLNPPCRFACRFSKPPFPPSQCNKPGNRKVMANSPPCRRDPTLQLTTS
                     HLWNLPLIGIERLLLHQQLAKTLNRPCPNDTHCCNQIGIVVCDENDSYYQLCCRKSLR
                     ELLLELLATQNNLQEELHLLIGQLYGNGHNDLKSRLSLSVCYLQDTLPPDALFIIRRT
                     HLEAFLTRHQPESKERITSALSRLLWLACKHNPQLDDEMLEHPYKLLNIFECWAREEG
                     MHLPLSPETLKTALQRGAPP"
     gene            1259..1453
                     /locus_tag="ACHOWS_003964"
     CDS             1259..1453
                     /locus_tag="ACHOWS_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0049466.1"
                     /translation="MNSDRFLRLRQVEDKIGFGKSWIYRQIQLQQFPPSIRLNSRHVA
                     WLESEVDAWIHQRIRLTRDV"
     gene            1470..2753
                     /locus_tag="ACHOWS_003965"
     CDS             1470..2753
                     /locus_tag="ACHOWS_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFQ0049467.1"
                     /translation="MCASHLLNRWLHGASNTGEQEMVQVSLEQIQPFSLNPRVTRNPG
                     YDVLKASILARGLDNPPVLTRRPGDEKYMLASGGNTRLAILNELWQETQDERYHRVCW
                     PFRPWPETLSPQQGEVQCLIGHLAESDLHNGLMFIERAEGVLHLRDLYLEAGIECPTQ
                     LALAEQLTRDGYPVSQSQISRMLQTVDWLLPCIPNALYGGLTRKVIDRLLSLRSAAEQ
                     VWVKLMPLERQPEFADLFSTALAIFNGEPEAVVPQLVQDELLGEMSCRSGMSWNTLLA
                     ELTDGQSKRQVLLGPPAEKVLWPPPPEVEKAPLPEPEPPTEPAAPDEPKPSPLVHTAQ
                     SLCACWGLDACIQPDDSAPAGFTLAPCHDFPHPAAQRCYQVLAALNGDTSTPMPALEL
                     LLSPDYNNAQIQQLMQLISLCRQRRLREVRHDTLS"
     gene            2737..3294
                     /locus_tag="ACHOWS_003966"
     CDS             2737..3294
                     /locus_tag="ACHOWS_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFQ0049468.1"
                     /translation="MTPSVNQAVLTHIVQALKEGQIRYCESLGFSPQELCELTRLTAD
                     DILFLSNSAVQFITTHIDHEMLGRMLARMEQERLFQQRLEEALSLGASIEFINHYFGL
                     STPEVCARRRLIGLFVRQGRNNAPDEQVETLVWNEWQKTGVKKLDSPEALTAMMAMAR
                     EHDLSLTVIWNLLRQWTQEKLLHEE"
     gene            3284..4156
                     /locus_tag="ACHOWS_003967"
     CDS             3284..4156
                     /locus_tag="ACHOWS_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFQ0049469.1"
                     /translation="MKSDDRLLFLGNPQQSVPKRLLLARNLSPRDKFAWQLLRLHARD
                     DGSGVFPSYNELQGWLSDQPEGEKASRSTVSNALMMLRITRWLSLCQRLRDEQSGRIT
                     GNLYALHDAPLTHADACRMDSEYLSLLTACSRHRNRRIRNIAIGLLLTLQQPVDGGRT
                     CTESPLKTAPGLNRGLSKKCPSLQLRLSNVAGAGGQPEPHCVHTGTDLNNKHQKRTPD
                     LHWPVDNPFSASERRAAERAMRNLDPALCQQVLDDCLWRIAAHDIHRPLAYLLATLEK
                     ARRGEFNQLRRRRR"
     gene            4316..4927
                     /locus_tag="ACHOWS_003968"
     CDS             4316..4927
                     /locus_tag="ACHOWS_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049470.1"
                     /translation="MPGTTLFSDIRITLHTRVAARLWQAHPTGMLLCLALLRRLLRAE
                     EADDPWAAHWLKQLRIRLNLIAHLLKQKNRRLDHAFASLPGAIHTTQVSNPSPTEFIL
                     PLALFSPPGSRLLQQLIDYDLLVRRTLLAWHLGLITQAEKRDFIATIPRLMLQVFSFV
                     NRFRTTGVTRADVRANSPLAQSMAHKLGNLPKKMLTEIQRDVQ"
     gene            4917..5342
                     /locus_tag="ACHOWS_003969"
     CDS             4917..5342
                     /locus_tag="ACHOWS_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HFQ0049471.1"
                     /translation="MFNDDDDERSARKSSLKGLLKAFKGKGELRALVQELQALRLQLQ
                     QNQPVLKQAVYELPGRNVPLILCSNPARSGVSYLRWRNLDNNRSGSVALQEAIQQPDA
                     SDELRDALMALEWRRHVLNTQLSVVMFMLRRIQPLTTLV"
     gene            5534..7339
                     /locus_tag="ACHOWS_003970"
                     /pseudo
     CDS             5534..7339
                     /locus_tag="ACHOWS_003970"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            7447..7629
                     /locus_tag="ACHOWS_003971"
     CDS             7447..7629
                     /locus_tag="ACHOWS_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049472.1"
                     /translation="MAKNTGDGYRKGSVTDRTQVQNPQNGNYTKRDTTTGRFIDQKQD
                     GTPFKGVAKEVDDRRK"
     gene            7913..8356
                     /locus_tag="ACHOWS_003972"
     CDS             7913..8356
                     /locus_tag="ACHOWS_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFQ0049473.1"
                     /translation="MNDTHYFNLYTSGLGYLNRIREVTPKKGEPFLCCDIAALRGEAS
                     NAEHTWFDCRVSGKEAQKLVRRCIDACEQKRKVLIQFRLGDLYADTFVYSKGDRKGET
                     GVSLKAHLLHIGLIKIDGEEVYRAEREAEPQPAPAALPAEPVAQF"
     gene            8430..8810
                     /locus_tag="ACHOWS_003973"
     CDS             8430..8810
                     /locus_tag="ACHOWS_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049474.1"
                     /translation="MNTVLNSREEALDRGKLINLDDEALDAGLPLDLAITLPAHKHCL
                     EDRNDNAIRDLLKGVEMAAVVAFISHQPPQRTIRIDYRSVLTQKTNGSTQQQTVRLTL
                     RVHFSEQGIPIAATLGLMGERFPY"
     gene            8924..9580
                     /locus_tag="ACHOWS_003974"
     CDS             8924..9580
                     /locus_tag="ACHOWS_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilL"
                     /protein_id="HFQ0049475.1"
                     /translation="MKRLLTALLLFIFNGVAASPATPEVVRYDRYLLVSTSPQQPPLE
                     QLTTLSVPPGFHPTVGEALQYLLRDSGWSLCLSDARRAQLYGLPLPLNQYHTGPLRLK
                     AALQLLAGPAWRMTADENRREVCFTSDHSLPQTVAYRHPVSLHPLGPLTAPFFLAGTV
                     QRGDIRLAVIVPPEALYLHQVVWLRIGEQWHGWRLARFDERRAVFRHGKRQLTLKVPE
                     "
     gene            9584..10258
                     /locus_tag="ACHOWS_003975"
     CDS             9584..10258
                     /locus_tag="ACHOWS_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049476.1"
                     /translation="MKRLILLLLFTASTWAQPLQVQTSQNVGSALQPLTTQQQATVWG
                     ITPEEWQRYLTLKQQARGVWSPGLDPLTTLGVEADTDHERQRFAELLVRQEYQRLEKE
                     LAFQRAYDAAWQRLYPGLTPLKTTSASTSRVSLFVKDDCPPCDSLLRTLLAQQRPLDI
                     WLVGSEGDDNRVRRWAMAHGIDAKLVGEHSITLNHDAGRWLLLGQSKIPVALERKGEQ
                     WREVLP"
     gene            10237..10755
                     /locus_tag="ACHOWS_003976"
     CDS             10237..10755
                     /locus_tag="ACHOWS_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFQ0049477.1"
                     /translation="MARGPALILLLPCLALAQPLPEAYLRTAEHFRVPPELLWAVTQT
                     ESGTNLSGRHLPWPWTLNISGTGYRYASREQACQALLVAIRRTSPKRIDVGLGQINIG
                     WHQAYFDSPCDALYPLQNLSLTAQLLRQRYDERPGSWLNAAARYHRPAGGAPAARYRK
                     LVSRHLQQASPP"
     gene            10752..12770
                     /gene="traD"
                     /locus_tag="ACHOWS_003977"
     CDS             10752..12770
                     /gene="traD"
                     /locus_tag="ACHOWS_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFQ0049478.1"
                     /translation="MSRYALEALLRPPVEFYSTVIAGCGALICLTQAPLLMLTPWQAR
                     MMASALMLLALRRGWQGWQIVRYQRNLLRLPRYALTSRKIPVSRQRLFLGKGFRWQPI
                     HTQRLYDCLQPEAARWLRPGWCYRLARHIEKRSEHTLPGLARLLSADRWLNPVRPLPP
                     VGGNPWLHGVELKECDVTLPLSERPGHTLVLGTTRVGKTRLAELLATQDIRRGEVVIV
                     FDPKGDADLLKRMWTEAQRAGRGDRFILFHLAYPEFSARYNAIGRFGRISEVASRLTG
                     QLSGEGNSAAFREFAWRFVNIIARALVALGQRPDYQLISRHVVNIDALFIDYARIVLP
                     RHNTRSWEVVVQLAERVNDKNTPRHMLGRDPHVVALEQYLNAEKLYDPILDGLLSAVR
                     YDKTYFDKIVASLLPLLEKLTTGTLAALLAPDYHDLDDARPILDWQQAIRQKAVVYVG
                     LDALSDSVVAAAVGNSMFADLVSVAGHLYKFGLNDGLPGNSEKPPPINLHCDEFNELM
                     GEEFIPLVNKGGGAGIQVTAYTQTLSDIQARIGSAPKTGQVVGNFNNLIMLRVRETAT
                     AELLTKQLPQVEVRSRQVSSGVTDTPGGINGFTSNTCEQVTTRNVPLLDTAALIQLPK
                     GQAFALLEGGQLWKIRLPLPVADKQCPLPDSVAQLAAWMQARQQGLTE"
     gene            12767..13414
                     /locus_tag="ACHOWS_003978"
     CDS             12767..13414
                     /locus_tag="ACHOWS_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFQ0049479.1"
                     /translation="MSELRPPPSRPPQGPIGWLLSLAGRIIGLVIGTLVLRVVLELVG
                     LYFWWPQEGSRHVFQVMRQEKVELIIAVQPHPLGKEIRVLLEHGITRVNDVRHKAVSR
                     LFHEPVTVLAYTMISFALRLTWLVIMLPLLCLSAVTGLTEGLVHRDLRRFGSGLESIF
                     LHRYVIRCGSSVTTTLWVVYLAQPFSLPAMLVLLPAALWSGIAVWMAAGSFKRWL"
     gene            13480..13725
                     /locus_tag="ACHOWS_003979"
     CDS             13480..13725
                     /locus_tag="ACHOWS_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFQ0049480.1"
                     /translation="MGVMSVRLNNNLSTQLEALSKATGRSKSWLANQAIEDYVAREAW
                     QVAEIEQAIQEADAGDFATSDEVDKLFQRLGVKPDGN"
     gene            13715..13999
                     /locus_tag="ACHOWS_003980"
     CDS             13715..13999
                     /locus_tag="ACHOWS_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0049481.1"
                     /translation="MEIKWLRKAIANLEAEFRYIAEDDPQAAERFVNEVWRLTQLLKE
                     QPAMGREGRVPETRELILHNYPYIIPYRVRNNIIQVLRVFHTHRRLPVNW"
BASE COUNT         3034 a         4340 c         3895 g         2750 t
ORIGIN      
        1 tcttcaaaat tcataacaga agcctttatc cattgatatt atactaatgg atatcatacc
       61 atatttatgc ctattcgtaa tcattgattt cctaaataaa acagtaaaaa acctaaaaaa
      121 aacaccaata gatcacatac aaaacaacca gttaaaagaa ctcaccttac ctcgctttag
      181 ttggcttttt tagatagtac tcatcatatg taactaatag gagttaccca tgcccccaca
      241 caaaattcgc caagagtggc tgacgctgga tgaggctgca gaagtcatcc ggcagcacaa
      301 catcacgttg ctgcccgccg acctgatccg ccatgccctg cacggcgatc tgtcgctctc
      361 ggtctacttc ccttcgcccg ttctgctgag accactcaat ccgccttgcc gctttgcctg
      421 ccgattcagt aagcctcctt ttccaccttc gcagtgcaac aaacccggca accgcaaagt
      481 gatggcaaac tcgccgccgt gcagacgcga cccaactctg caactgacca ccagccatct
      541 gtggaatttg ccgttaatcg gcattgagcg cctgctgctc caccagcaac tggcgaaaac
      601 gttgaatcgc ccttgcccca atgataccca ttgctgcaac cagattggca tcgtggtctg
      661 cgatgaaaac gacagctact accaactctg ttgccgcaaa tcgttgcggg agcttttact
      721 ggaactgctc gccacgcaaa acaacctcca ggaagagctt catctgctga ttggccagct
      781 atatggtaat ggccacaatg acctgaaatc ccggctcagt ctgtccgtct gctacctgca
      841 ggacacgctg cctcctgacg cactgttcat catccgccgc actcatctcg aagccttcct
      901 gacccgtcat cagccagaat caaaggagcg gatcacctcc gcgctttcgc gtttgctgtg
      961 gctggcctgc aagcacaacc cgcaactgga cgatgaaatg ctggagcatc cctacaaact
     1021 gctgaatatt ttcgaatgct gggcgcgtga ggaaggtatg cacctgccgc tgagccccga
     1081 gacgttgaaa accgcactcc agcgcggcgc accgccgtaa cgcgatcaac taaccctttc
     1141 aactaacaac tctcactaac ccagataagg atttttactg cctggcaggg acgctgctgc
     1201 tttgataacc acgcctatcg tgctgatgtg aacgaatctg gacacaggag gaatggttat
     1261 gaatagcgac agatttttac gcttacgcca ggtggaagat aaaatcggct tcggtaaatc
     1321 gtggatttat cgacaaatcc agctgcaaca gtttccgccg tccattcgac tcaatagccg
     1381 ccacgtcgcc tggctggaaa gcgaggtgga cgcgtggatc caccagcgca tccggcttac
     1441 ccgcgatgtc tgaatgccgg aggaagatta tgtgcgcttc acacctgctc aaccgctggc
     1501 ttcacggcgc cagcaatacc ggagaacagg aaatggtcca ggtcagcctg gagcaaatcc
     1561 agccgttctc cctcaatccc cgcgtcaccc gcaaccccgg ctatgacgtg ctgaaggcgt
     1621 caattcttgc ccgtgggctg gataacccgc cggtactgac ccgccgcccc ggtgatgaaa
     1681 aatatatgct cgccagcggc ggaaatacgc gcctcgccat cctcaatgaa ctgtggcagg
     1741 agacgcagga tgagcgttac caccgcgtct gctggccgtt cagaccgtgg ccggaaacgc
     1801 tgtcaccgca gcaaggcgaa gtccagtgcc tgatcggcca tctggccgaa agcgacctgc
     1861 ataacggatt gatgtttatc gaacgggcgg aaggggtcct gcacctgcgc gatctttacc
     1921 tcgaggcagg cattgaatgc ccgacgcagc tggcgctggc ggaacaactc acccgcgacg
     1981 gctatcccgt cagccagtcg caaatcagcc ggatgctaca gaccgtagac tggctgctgc
     2041 cctgcatccc aaacgcgcta tatggcggtc ttacccgcaa ggttatcgat cgcctgctgt
     2101 cactacgcag cgcagcagaa caggtctggg taaagctgat gccgctggaa cgccagcccg
     2161 aatttgccga cctgttcagc accgccctcg ccatcttcaa cggcgagccg gaagccgtgg
     2221 taccgcaact ggtgcaggac gaactgctcg gtgagatgag ctgtcgcagc ggcatgagct
     2281 ggaatacgtt gctggcggaa ctgactgacg gtcagagcaa gcggcaggtg ctgctggggc
     2341 ctccggccga aaaggtcctc tggccgccac cgccggaagt ggaaaaagca ccactaccag
     2401 agcctgagcc accgactgaa cccgccgcgc ccgatgagcc aaagccctca ccgctggtgc
     2461 atacggcaca gtcgctgtgt gcctgctggg gactggacgc ctgtatccag ccggacgaca
     2521 gcgctccggc cggtttcacc ctcgcgcctt gccacgattt tccccacccg gcggcacagc
     2581 gctgctatca ggtgctggcg gccctgaatg gcgacaccag tacgccgatg cccgccctcg
     2641 aattgttgct gtcgccggac tacaacaacg ctcagatcca gcaactgatg cagcttatta
     2701 gcctgtgccg ccagcggcgg ctgcgggagg tgcgccatga caccctcagt taaccaggca
     2761 gtactcaccc acatcgtcca ggcgctgaag gaggggcaga tccgctactg cgaatcgctc
     2821 ggcttcagcc cgcaggagct gtgcgagctg acgcgcctta ccgccgacga catcctgttt
     2881 ctcagcaaca gcgcggtgca gttcatcacc acccacattg atcacgagat gctgggccgg
     2941 atgctcgcga ggatggagca ggagcggctg ttccagcagc gtctggagga agcgctgtcg
     3001 ctcggcgcgt ccatcgagtt cattaaccac tattttggcc tttccacgcc ggaggtctgt
     3061 gcccggcggc ggctgattgg cctgtttgtc cgccaggggc gcaacaatgc cccggatgag
     3121 caggtggaga cgctggtgtg gaacgagtgg cagaagacgg gcgtgaaaaa gctcgactca
     3181 cccgaagcgc taaccgccat gatggcaatg gcccgcgaac acgatctgtc gctgacggtc
     3241 atctggaacc tgctgcgcca gtggactcag gagaagctgc tgcatgaaga gtgacgatcg
     3301 gctgctgttt ctcggcaacc cgcagcagag cgtgccgaag cgcctgctgc tggcgcgcaa
     3361 cctctccccg cgcgacaagt tcgcgtggca actgctgcgc ctgcatgccc gcgacgacgg
     3421 cagcggcgtc ttcccctcct acaacgaact gcagggctgg ctctctgacc agcctgaagg
     3481 cgagaaggcc tcgcgcagta ccgtcagcaa tgcactgatg atgctgcgga tcacccgctg
     3541 gctgagcctg tgccagcggc tgcgcgacga gcaaagcggc aggatcaccg gcaacctcta
     3601 cgccctgcat gatgcgccac tgactcatgc cgatgcctgt cggatggaca gtgaatacct
     3661 gtcgctgctc accgcttgtt cccgccaccg caaccgacgc atccgaaaca tcgctatcgg
     3721 gctgctgctc accctgcaac aacctgtgga cggtggacga acctgcactg agtctccatt
     3781 gaagaccgcc cctggtctta atcgaggact cagtaaaaaa tgcccgagtc ttcaattaag
     3841 actcagtaat gtggcggggg ctggcgggca gccggagccg cattgcgtac atactggtac
     3901 agatttaaac aataaacatc aaaagcgtac gccagacctg cactggcctg tggataatcc
     3961 cttcagcgcc agcgagcgcc gcgccgcaga acgcgcgatg cgcaatctgg atcccgctct
     4021 ctgccagcaa gtgctggacg actgcctgtg gcggatcgcc gcccacgata tccaccgccc
     4081 cctcgcctat ctgctggcaa cgctggagaa agccagacgg ggcgagttca atcagcttcg
     4141 gcgcaggcgc agatagccct gattttatgc gcaccgcata aagccggtgt accaactatc
     4201 aatcaaacct cattcatcgg cgaaattaac tttctgcgga ccagatgggt ttcccgtcgc
     4261 ctgactcatt gtgcggaccg acactgcggg catctgaatc catggagaat ttccgatgcc
     4321 tggcacaacc ctgttcagcg atatccgcat caccctgcat acccgcgtcg ccgcccggct
     4381 gtggcaggcg catcccactg gcatgctgct ctgtctggcg ttactcaggc gtctgctgcg
     4441 cgccgaagag gccgacgatc cgtgggccgc ccactggctg aagcaactgc gcatccgcct
     4501 gaacctgatc gcccacctac tgaagcaaaa aaaccgtcgt ctggaccatg ctttcgccag
     4561 cctgccaggc gctatccaca ccacgcaggt cagtaacccg tccccgaccg aattcatcct
     4621 gccgctggcg ctattctcac cgcccggttc gcgcctgctg caacagttga ttgattacga
     4681 tctgctggtc cgccgcaccc tgcttgcctg gcatctcggc cttatcaccc aggcggagaa
     4741 gcgcgatttc attgccacca tcccacgcct gatgctccag gtgttcagct tcgtgaaccg
     4801 ctttcgcacc actggcgtga ctcgcgccga cgtgcgggcg aacagtccgc tggcgcagtc
     4861 gatggcgcat aagctcggaa atttgcccaa aaagatgctt acggagatac agcgcgatgt
     4921 tcaatgacga tgatgatgaa cgctccgccc gcaaaagctc gctaaagggc cttttaaagg
     4981 cctttaaggg taagggagag ctgcgcgcgc tggtacagga attacaggcg ctgcgcttgc
     5041 agctgcaaca aaatcagcca gtgctgaaac aggcggtgta tgaactgccg gggcgtaatg
     5101 taccgctgat tctgtgcagc aacccggcgc gctccggcgt cagctatctg cgctggcgca
     5161 atctggacaa caaccgttcg gggagcgtgg cgttgcaaga ggccattcaa cagccggacg
     5221 cgagcgatga actgcgcgac gcgctgatgg cgctcgaatg gcggcggcat gtgctgaata
     5281 cgcagctttc agtggtgatg tttatgctgc ggcggataca accgttaacc accctggttt
     5341 aaaattttgc gcaaagttaa accctgagtg tcactttatc gattatttta aacttaactc
     5401 atgcacctta aaacaggcac tgtgcgctta atgcacgttt taaggtgcaa tttttacctt
     5461 ctcctaccag tttcccctgt cgccttatcc ctccggcaca ccatgctgaa ctccctttca
     5521 ccggagacag cccatgcaac tttacctgtg cgaaaaaccc tctcaggcac gcgacatcgc
     5581 ccgaattctt gacgcccata accgcagcga cggctacctg cataacgcca ccgtaaccat
     5641 cacctggtgt ttcggccatc tgctgagcat ggccgcgccg gaggattacg atccggcgct
     5701 gaaacactgg cggacggaac agctgccgtt tattcccccg gcatggcggt tggtgatccg
     5761 cccggaggtg cataagcagt tccgtatcat cgaaaagctg ctgaaacagg cggatgcagt
     5821 ggtgattgcc accgacgccg atcgcgaagg cgaaaccatc gcccgcgaaa ttctggaaca
     5881 atgccgctgg cgcggtccgg tccagcggct gtggctgtcg gcgctggatg aggccagtat
     5941 ccacaaggcg ctcgccactc tgcgtcccgg cgacagcacc tttccgctgt atcaggccgg
     6001 actggcgcgc gcccgcaccg actggctggt cggcatcaac atgacccgcg cctgcacgct
     6061 aatgaaccgg cggcacaagg gcgtgctctc cgtgggccgg gtgcagacgc cgaccctgcg
     6121 tctggtggtg gagcgcgacc gggagatcgc ccgcttcacg ccccgcccct ggtggcgggt
     6181 ggatgcccgt ttgcatgcgg agggttgttc ctttctggcg cagtggcagc cggatagtgc
     6241 gtactgtgac gacgaaagcc gctgcatccg cgaatccgcc gcccgtgagg ccacagcacg
     6301 cctgcaacaa gccggaaccg cgctggtaat ggacgtgaca acccggcggg agaaaacgcc
     6361 gccaccgcag gccttcactc tcagcgccct gcagcaggcc tgctcaaaac agtggggcat
     6421 gggcgcacag gaggttctgg atatcgccca gctcctgtat gaaacccata aagccaccac
     6481 ctacccgcgc accgactgcg gctatctgcc gctctccatg cacgatgaag caccacaagt
     6541 tctggcggcg ctggtcgcca gcgacgcttc actccagccc ctcgccacgc agcttaatct
     6601 gcagcaacgc tcccggttgt gggacgacag caaaatcacc gcccaccatg gcatcatccc
     6661 gacccgtaaa acggtagatc tgacgcagat gaacgacagc gagcgcaagg tctacggcct
     6721 ggtacgcagc cattttctgg cgcagtttct gccagaccac gaagtggata aaacggacct
     6781 gcgcctgaac tgcgccgggg aaacgctgac tgcccgtggc tcagtggtga ttgtcagcgg
     6841 ctggcgacaa ctgtttttgc gcgaaatggc aacgcactca ccggaagaaa cgcaggcact
     6901 tcccgccctg caacagggac aaacctgcta ggtggaacag gtggacgttc gcgcccgcca
     6961 gacccagcca ccggagcact acaccgaagg gacgctgatt gccgcgatga aaaatgccgc
     7021 ccgttttgtc agcgatgaac gcctgaaaca gcggctgaaa gaaaacgcgg gcatcggcac
     7081 cgaagccacc cgcgccggga ttatccagac gctccttaag cgcggctatc tgattaagca
     7141 gcgtcgtttc ctgctggcaa cggataccgc cagcacgctg attgacgccc tgccggaaac
     7201 gctgaaagat ccaggcacca ctgcactgtg ggagcaaatg ctggatgaca tcgcgacggg
     7261 gaaaatggga ctggaggcgt tcctggtgca gcaacagcag aacgtgacag agctgattaa
     7321 gtcgttacgg aatgagtaaa taacctgttt cctgcatcac acttttactt ttccctgatt
     7381 gctcgttcaa attggcagtc tgatgatcct ctctgaggaa tattcctcat ttttcggagt
     7441 gttaagatgg ccaagaatac cggagacggt tatcgcaagg gctctgttac agaccgcaca
     7501 caggttcaga atccgcagaa tggcaactac accaaacgcg atacaacgac aggtcgcttt
     7561 atcgaccaga agcaggacgg cactcccttt aaaggtgtcg ccaaagaagt cgatgaccgc
     7621 cggaaataat atttagctaa ctttcagggc cttagttatt aaggcctttt tgcattctgt
     7681 acttccaact ttcacgccgt aaaaacaatt ccacctttcg tttctcccta ccgaagattc
     7741 aactaaacag gtcaacccca aaacccgcca tgcattcctg aaacgcggag gcgcacagcg
     7801 cggaatgccc gtactgccag taactcagag gccttgcagc caatgagcca aacccgaagc
     7861 ggggcgttcc cgcgcatggg gcgctccgca ccttcaacac ggagatacca ccatgaacga
     7921 tacccactac ttcaacctgt acaccagcgg tctgggctac ctcaaccgga tccgggaagt
     7981 gaccccgaaa aaaggcgaac cgttcctgtg ttgcgacatt gccgcactac ggggcgaagc
     8041 cagcaacgcg gagcacacct ggttcgactg ccgcgtcagc ggcaaggaag cgcagaaact
     8101 ggtgcgccgc tgtatcgacg cctgcgagca gaaacgcaag gtgctgatcc agttccgcct
     8161 cggcgatctg tacgccgaca cctttgtcta cagcaaaggc gaccgtaaag gcgaaaccgg
     8221 cgtcagcctg aaggcccatc tgctgcacat cgggctcatc aaaattgacg gtgaagaggt
     8281 ttaccgggca gagcgcgaag ctgaacctca gccagcgcca gccgcactgc cagcagagcc
     8341 tgtagcgcag ttctgacgca cagcattcac cctgccgggg aaattccccg tcggggcgtt
     8401 tccctggcct tttaccggag agacgcacga tgaataccgt attgaactca cgcgaagaag
     8461 cccttgaccg gggcaaactg attaaccttg atgacgaggc gctggacgct ggcttgccac
     8521 tggatctggc cattaccctt ccggcccaca agcactgtct tgaagaccgg aatgacaacg
     8581 ccatcaggga cttgctgaaa ggtgttgaaa tggcagcggt agtggccttt atctcccacc
     8641 aaccgccgca gcgcaccatt cgtatcgatt accgctcggt gctgacgcaa aaaaccaacg
     8701 gcagcacgca gcagcaaacc gtcagattga cgttacgcgt tcatttttct gagcaaggca
     8761 tccccatcgc cgccacgctg gggctgatgg gggaaagatt cccttactga tctgattttg
     8821 cgcccggaat aacgggcgct attttttgac gcagcaaaca gttgtcggta gtccccagcc
     8881 ctctcatccg gcacgctcct gtaaaagcac tgtggaggat gcgatgaaac gactgctgac
     8941 agctctgctc ctgtttattt tcaacggcgt ggccgcctcc cctgcaacgc cagaagtcgt
     9001 ccgctacgac cgctatctgc tggtcagcac gtcaccgcaa caaccgccac tcgaacagct
     9061 taccaccctg tccgttccgc ccggttttca cccgacagtc ggtgaagcat tgcagtacct
     9121 gctgcgcgac agcggctggt ctttgtgtct ctccgatgcg cgccgggcgc agctttatgg
     9181 cctcccgtta ccgttgaacc agtaccacac cggcccgctg cgcctgaagg ccgccctgca
     9241 actgctggca ggcccggcat ggcgaatgac cgcagacgaa aaccgccgtg aagtctgttt
     9301 taccagcgac cattccctcc cccagacggt ggcttaccgt catcccgtgt ccctccaccc
     9361 tttggggccg ttaacggccc cgttttttct cgccggaacc gtacagcgcg gcgatatccg
     9421 actggcagtg atcgtgccgc cggaggccct ttatctccat caggtagtgt ggctgcgtat
     9481 cggggagcag tggcacggct ggcggctggc gcggtttgat gagcgccggg cggtattccg
     9541 ccacggtaaa cgccagctga cgctgaaagt accggagtaa gcgatgaaac gcctgattct
     9601 cctgttactg ttcaccgcca gcacgtgggc gcaaccgctt caggtgcaga ccagccagaa
     9661 cgtgggttca gccctgcaac cactgaccac gcagcagcag gccaccgtct ggggcatcac
     9721 gccagaggag tggcagcgct atctcacgct gaaacagcag gcgcgcggcg tctggtcgcc
     9781 ggggctcgat ccactgacca cactcggcgt agaagccgac actgaccacg aacgccagcg
     9841 ctttgccgaa ctgctggtca ggcaggagta ccagcggctg gagaaagaac tcgccttcca
     9901 gcgtgcttat gatgccgcct ggcagcgcct gtatccgggc ctgacgccgc tgaaaaccac
     9961 ctctgccagt acttcccgcg tttcgctatt tgttaaagat gactgcccgc cctgcgacag
    10021 cctgctgcgt accctgctgg cgcaacagcg cccgctggat atctggctgg ttgggagtga
    10081 aggcgatgat aaccgggtgc gccgatgggc gatggcgcat ggcatcgacg caaaactggt
    10141 gggtgaacac agcatcacgc ttaaccacga cgcgggccgc tggctcctgt tggggcagag
    10201 caaaatcccg gtggcgctgg aacgtaaggg ggagcaatgg cgcgaggtcc tgccctgatc
    10261 ctgctgctac cctgtctggc gctggcacag ccgctgccag aagcgtacct gcgcaccgcg
    10321 gagcatttcc gcgtgccgcc ggagctgctg tgggccgtca cacaaaccga aagcggcacg
    10381 aacctgagcg gcagacatct tccgtggccg tggacgctca atatcagcgg gaccggctac
    10441 cgctatgcca gccgggagca ggcctgccag gcgctgctgg ttgccatccg ccgcaccagc
    10501 ccaaagcgca ttgacgtggg actcgggcaa atcaacatcg gctggcacca ggcatatttt
    10561 gactctccct gcgacgccct gtatcccctt caaaacctgt cgctcaccgc gcaactgctg
    10621 cgtcagcgct acgatgaacg tcccggaagc tggctcaatg ccgccgcccg ctatcatcgt
    10681 cccgctggcg gcgcgcctgc cgcgcgatac cgcaagctgg tcagccgcca tctgcaacag
    10741 gcctcaccgc catgagccgc tacgcgctgg aggcgctgct gcgcccaccc gtggagtttt
    10801 acagcaccgt catcgctggc tgtggcgcat taatctgcct gacgcaggcc ccgctgctga
    10861 tgctgacgcc gtggcaggcc cgcatgatgg ccagtgccct gatgcttctg gccctgcggc
    10921 gcggctggca gggatggcaa atcgtacgct accagcgtaa cctcctgcgc ctgccgcgct
    10981 acgcgctcac cagccggaaa atccccgtca gccgccagag gctgtttctc ggtaaaggct
    11041 ttcgctggca gcccatccac acacagcgtc tgtacgactg cctgcaaccg gaagccgcac
    11101 gctggctgcg ccccggatgg tgttaccgcc tggcaagaca catcgaaaaa cgcagcgagc
    11161 ataccctgcc cggccttgcc cgactgctca gcgccgatcg ctggctgaac ccagtacgtc
    11221 cgctgccacc cgtaggcggc aatccgtggt tgcatggggt ggaactaaaa gagtgcgacg
    11281 tcactctgcc gctcagtgaa cgccccggcc atacgctggt gctcggcacc acccgcgttg
    11341 gcaaaacccg gctggcggaa ctgcttgcca ctcaggacat ccgccgtggc gaggtggtga
    11401 tcgtttttga tccgaaaggc gacgcggatc tgctgaagcg tatgtggaca gaagcgcagc
    11461 gcgccggacg cggcgaccgc tttatcctgt tccatctggc gtacccggag ttctccgccc
    11521 gctacaacgc tatcgggcga tttggccgca tttcggaggt ggcgtctcgc ctcactggtc
    11581 agctttccgg cgaaggcaac tcggcggcct ttcgcgagtt cgcctggcgc tttgtcaaca
    11641 tcatcgcccg cgccctggtg gcgctcggcc agcgccccga ctatcagtta atttcccgcc
    11701 acgtcgtcaa tatcgacgcc ctgtttatcg actatgcccg catcgtgctg ccgcgccaca
    11761 acacgcggtc atgggaagtg gtggtgcaac tggcggagcg cgttaacgac aaaaacacgc
    11821 cgcgccacat gctgggccgc gatcctcacg tggtggcgct ggagcagtac ctcaacgcgg
    11881 aaaaattata cgatccgatc ctcgatggcc tgctctcggc ggtgcgctat gacaaaacct
    11941 atttcgacaa gattgtcgcc tcactgctgc cactgctgga aaagctgacc accggcacgc
    12001 ttgccgccct gctggccccg gattaccacg atctggacga cgcgcgtccg atcctcgact
    12061 ggcaacaggc gatccgccag aaggcggtag tctatgtcgg gttggacgcg ctctccgatt
    12121 cggtggtcgc tgccgccgtc ggcaattcga tgtttgccga tctggtgagc gtcgccgggc
    12181 atctgtataa atttggcctc aatgatggac tgcccggcaa tagcgaaaaa ccgccgccta
    12241 tcaatctgca ctgcgatgag tttaatgaac tgatgggcga ggagtttatt cccctggtca
    12301 acaagggcgg cggtgcgggc attcaggtca ccgcctacac gcagacgttg agtgatattc
    12361 aggcccgtat cggcagcgca ccaaagacag gccaggtggt gggtaacttc aacaacctga
    12421 ttatgctgcg ggtacgcgaa accgccaccg ccgaactgct gacgaagcaa ctgccacagg
    12481 tggaagtgcg cagccgccag gtcagttcag gtgtcaccga cacgccgggc ggcatcaacg
    12541 gctttaccag caacacctgc gagcaggtga ccacccgcaa tgtaccgctg ctggataccg
    12601 ccgcgctgat ccagctcccc aaaggccagg cttttgccct gctggaaggc gggcagttgt
    12661 ggaaaattcg tctgccgctg cccgtagcgg ataagcaatg cccactacca gattctgtcg
    12721 cgcaactggc ggcgtggatg caggcgcgcc agcaggggct aacggaatga gtgagctgcg
    12781 accgcctccg tcacgcccac cacagggacc gattggctgg ctattgtcac tggccggacg
    12841 gattattggg ctggttatcg gtacgctggt gctgcgagtg gtgctggaac tagtggggct
    12901 atatttctgg tggccgcagg aagggagcag gcatgttttt caggtgatga ggcaggagaa
    12961 ggtggaactg attattgcgg tacagccaca tcctttgggg aaagagatac gagtactgct
    13021 ggagcatgga ataacccgcg ttaatgatgt cagacataag gccgtgagtc ggttgttcca
    13081 cgaacccgtt accgtcctgg cttataccat gatcagcttt gcgctgcgcc tgacgtggct
    13141 ggttatcatg ttgccactgc tgtgcctcag tgctgtaacc gggctgacag aaggcctggt
    13201 gcaccgggat ttaagacggt ttggctccgg gctggaatcc atttttctcc accgttatgt
    13261 catcaggtgc ggcagttcag tcaccaccac gctttgggtg gtttatctgg cgcagccatt
    13321 ttctctgccc gcgatgctgg ttttacttcc tgccgcctta tggtccggga tagcggtatg
    13381 gatggcagca ggaagcttta aacgttggtt ataagttgct caaaaaatcg cacctcacta
    13441 caatgtatac aatgtgtaca tgtgtatgag gccaccaata tgggcgtaat gtcagtccgt
    13501 ttaaacaaca atctttccac gcaactggaa gcattatcaa aagcgaccgg acgctcaaag
    13561 tcctggctgg caaatcaggc aattgaagat tatgttgccc gggaggcctg gcaagttgca
    13621 gagattgagc aggccattca ggaagctgat gccggtgatt ttgccacttc agatgaggtg
    13681 gataagctgt ttcaacgtct gggagtaaag ccagatggaa attaagtggc tacgtaaagc
    13741 cattgccaat ctcgaagctg agttccggta tattgctgaa gacgatccgc aggctgcgga
    13801 acggtttgtg aacgaagtct ggcgacttac gcaactgctc aaagagcaac ccgctatggg
    13861 ccgggaagga agggtacctg aaacacgtga gctcattctt cataattacc cttatatcat
    13921 tccctatcga gtacgaaata atataattca ggttttacgc gtgtttcata cccatcggcg
    13981 cttacctgta aattggtgat aaaaaacaca agacaagaa
//
LOCUS       DAZKJR010000129        13963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKJR010000129 DAZKJR010000000
VERSION     DAZKJR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.130"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..1226
                     /gene="tssD"
                     /locus_tag="ACHOWS_003981"
     CDS             93..1226
                     /gene="tssD"
                     /locus_tag="ACHOWS_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFQ0049482.1"
                     /translation="MSDIIYLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFI
                     PRQKTKDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFKPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            1223..1480
                     /locus_tag="ACHOWS_003982"
     CDS             1223..1480
                     /locus_tag="ACHOWS_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0049483.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            1555..1800
                     /locus_tag="ACHOWS_003983"
     CDS             1555..1800
                     /locus_tag="ACHOWS_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0049484.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(1969..4230)
                     /gene="metE"
                     /locus_tag="ACHOWS_003984"
     CDS             complement(1969..4230)
                     /gene="metE"
                     /locus_tag="ACHOWS_003984"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0049485.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4348..5301
                     /gene="metR"
                     /locus_tag="ACHOWS_003985"
     CDS             4348..5301
                     /gene="metR"
                     /locus_tag="ACHOWS_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFQ0049486.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5189..6088)
                     /gene="bioP"
                     /locus_tag="ACHOWS_003986"
     CDS             complement(5189..6088)
                     /gene="bioP"
                     /locus_tag="ACHOWS_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFQ0049487.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(6164..6964)
                     /gene="yigL"
                     /locus_tag="ACHOWS_003987"
     CDS             complement(6164..6964)
                     /gene="yigL"
                     /locus_tag="ACHOWS_003987"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFQ0049488.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6972..7994)
                     /gene="pldB"
                     /locus_tag="ACHOWS_003988"
     CDS             complement(6972..7994)
                     /gene="pldB"
                     /locus_tag="ACHOWS_003988"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFQ0049489.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVIVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8105..8725
                     /gene="rhtB"
                     /locus_tag="ACHOWS_003989"
     CDS             8105..8725
                     /gene="rhtB"
                     /locus_tag="ACHOWS_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFQ0049490.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKPL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8787..9407)
                     /gene="rhtC"
                     /locus_tag="ACHOWS_003990"
     CDS             complement(8787..9407)
                     /gene="rhtC"
                     /locus_tag="ACHOWS_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFQ0049491.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9471..11300)
                     /gene="recQ"
                     /locus_tag="ACHOWS_003991"
     CDS             complement(9471..11300)
                     /gene="recQ"
                     /locus_tag="ACHOWS_003991"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0049492.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENDVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPISASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11432..12301)
                     /gene="pldA"
                     /locus_tag="ACHOWS_003992"
     CDS             complement(11432..12301)
                     /gene="pldA"
                     /locus_tag="ACHOWS_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFQ0049493.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITRHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12466..12933
                     /gene="yigI"
                     /locus_tag="ACHOWS_003993"
     CDS             12466..12933
                     /gene="yigI"
                     /locus_tag="ACHOWS_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFQ0049494.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12985..13875
                     /gene="rarD"
                     /locus_tag="ACHOWS_003994"
     CDS             12985..13875
                     /gene="rarD"
                     /locus_tag="ACHOWS_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFQ0049495.1"
                     /translation="MDTKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPKKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTAK"
BASE COUNT         3519 a         3617 c         3462 g         3365 t
ORIGIN      
        1 attttagttc atcccttttg ggcgtaaata aaggtaaaaa ataacaatac tatttttctt
       61 tcacaaaaga attaacaaaa ggtttttcaa ggatgagtga tattatttac ctgaccataa
      121 ccggagaaca acaaggatgt atttcctctc gttgcggaac gagcgcatca ataggtaatc
      181 gctggcagat tggccatgaa gatgaaatct tcgcgttctc gctatctaat agtattacga
      241 atacaggcaa agggtcacaa ttacatggcc ttagtttctg taagttgatt gacaaaagct
      301 ccccgttact tattaacgct attaacaata acgagcagtt gttcatggag tttgacttct
      361 atcggatcaa tagatttggt cgatgggaga aatattacaa cattcaactc cgcggcgcgc
      421 tcttatcagc aatcaatcac ctgtttactg agaacaatct ggatacagaa accatcactg
      481 tcagttatga atatatactc tccaggcatc tgattgctaa tactgaattt agtcatttgg
      541 catttcccga taactacaac cgtctgttta taccacggca aaaaactaaa gatgataatg
      601 gcctcaaaac actcaatagt aaagcggttg gcagactgct tgctgctggc ggcatataca
      661 atgggaatat cgaaggattc agggaaacag caaacaagtt gggaggagat gcacctgctg
      721 gttacgatca agttatggat aacaaagggt tactcatcgc tggtgcttca atagttgctg
      781 gagttgttct gggaagaatg agattccctg atttggaatc atttgagcat ttcggagcca
      841 gaggagcagt atcaggaaga ccatttaagc ccgaccttgc cggtggtcca atagaaaacc
      901 tgacgataga tggcgtaaca atcaataggg aaggtatcgc tattgttgaa aagcatatct
      961 ccagatttga tcatgatcca gctaatgatg taatgatttc aaggctaaaa aaaatagcaa
     1021 ataaagaatt gcttcctgaa aaatacgatc tgaactacta tactcatgaa tgccgcgagt
     1081 atcagcgtta ttgtaattta ggatgggaaa caggtgaacc gaatggttta gatggatatg
     1141 aattatggaa taatgcacat actgcaactc tagaagactt taaaattaaa gatactgact
     1201 tatttcatcc ggatgcaaaa aaatgacgat tacgatagtt gaatttaacg tgctgaaagt
     1261 tatggctgag aaagatattg actggagctg gatggtgctg gatcgtactt tagctatacg
     1321 aaatattcca ggatttggca acgtagcgaa tatcgtaaca aagctggtta atcatgggtt
     1381 ggttgatatc gtaaatggtg agggcaactc taaaccacgc tacagagttt ctcaatatgg
     1441 attgaatctt atcaaagaac aacaagacaa cctcttctga tccagccatt acaggagcat
     1501 gatttacatt gtgatctttc aataagatac tcatcaattt tggtacagaa tatcgtgatc
     1561 actaaaaatg aacttaacgt gctgaacgtt atgactgaga aagatataaa ctggaactgg
     1621 atgaatctcg atcgcacact atccataagg caaataccag gattcggtaa tgttgttaat
     1681 attgttaaca aattagctaa cgaaggtttg gttattattg aggatagcgg gcataagtct
     1741 atgcctcact accacgttag tgaaaaggga tacaatctcg tgcaacgtga gaataaataa
     1801 cgtagcgttg attactgaac ggaatcactc aaaactgtgt tttgcgaaaa aaacgattcg
     1861 gttatttcta ctcctgaaga tctgcttcaa tctggcactc atgcaaaatg gcttccagta
     1921 ctggaagcca ttatcgtact gcaatttaca atgactcgtt aatactcatc atccccgacg
     1981 caaattctgc gccgcctgca ccatgttcgc cagtgccgcg cgggtttctg gccagccgcg
     2041 cgttttcagg ccacagtccg ggtttaccca cagacgctct gccggaatgc gttttgccgc
     2101 tttcttcagc agggcttcaa tccattccac gctcggtacg tttggcgagt gaatgtcata
     2161 gacgcctgga ccaatttcat ttggataatc aaactcttca aacgactcca gcaactccat
     2221 gtcggaacgc gaggtttcga tggtgatgac gtctgcgtcc agcgccgcaa tcgaatccat
     2281 gatgtcgttg aactcgcagt aacacatgtg agtgtggatt tgtgtgtcat ctttcgccac
     2341 ggcggcgttg atacggaaag cctccacgcc ccactggaga taggcatccc agtcgctgcg
     2401 acgcagcggt aaaccttcgc gcagtgccgg ttcatcaatc tggatgatgc caattccagc
     2461 ggcttccagg tccgccactt catcacgcag cgccagcgca atttgtttgg cgatggtttc
     2521 acggctgaca tcttcacgcg ggaatgacca gcagagtatg gttaccggcc ccgtcagcat
     2581 ccctttcacc ggtttgtcgg tcagcgattg tgcatatttc gcccactcca cggtaattgg
     2641 tgccgggcgg ctaacgtcac caatgacaat cggtggcttc acgcagcggg aaccgtagct
     2701 ctgtacccaa ccgttttgcg taaagacaaa gccatccaga tgctcgccaa agtattccac
     2761 catgtcatta cgctcggcct cgccatgtac cagcacatcc agtcccaaac gttcctgctc
     2821 gacaatggcc tgcttgatat gttccgcaat gcccgtgcgg tagttgttgg cgtcgagatt
     2881 accctttttg aaatccagac gcagtgtacg aatttccgtg gtttgcggga aggaaccaat
     2941 cgtggtagtc ggccacgcgg gcagtttaaa acgcgcacgc tgggcttcag cacgcacttc
     3001 atagacattc gcacgctgac tgtcctgggc ggtaatggct gcaagacgct tttctaccgc
     3061 cggattatgg acacgggtag agtgacgacg cgcctgaatc ggagcgctcc attctgccag
     3121 agccgccgta tcaccactgt tcagcgcatc gcgcagcagt gccagttcat ggcatttttg
     3181 tagggcgaag gcaaaccagc ttttcacttc tgcatcaaga cgcgtttcca cgctcaggtc
     3241 gatggggctg tgcagcaacg agcaggaaga tgccacccac aaatcgcgtt tgccgacaat
     3301 gtccttaatt tgcgcatatt tctcggtaag atcggcgcgc cagacgttac gaccattgat
     3361 cagacccgca gacagtagcc agtcagaagg cagacgcttg tgcagttcag caacgtcatc
     3421 tttaccatgt acaaggtcca catgcagccc ctgaacaggc agcgcagtaa tcgtgtcgag
     3481 atttggcgtt acgccttcaa aataggtagt cagcagcagt ttcacctggc cctggagcgc
     3541 gtcgtaagcg ggtttgtatg cgtccagcca cgcctgcggt agttccagta ccagcgctgg
     3601 ttcgtcgatc tgtacccact caatgccgcg ttttgccagt tctgccaaca cttgctgata
     3661 aaccggcaga atgtcgttca gcaggctcag gcgatcaaac tgttcacctt tcactttacc
     3721 cagccacagg taggtaaccg gccccagcag cacgggcttg accttgtggc ccagcgccag
     3781 cgcctcgtcc acttcctcca gcagctgcgt ccaggtcagt ttgaactgtt ggcctttaac
     3841 gaactccggc accatgtagt gatagttggt gttaaaccat ttggtcattt ccgctgccgc
     3901 cgcaggttcg ccagtcggtg cacgtccacg accaatacgg aacagggtgt cgatatctac
     3961 cgaaccatct ttgttctgat gacgtggcgg aacgttaccc agcagcagac tggtggtcag
     4021 tacatgatcg taccaggcaa aatcgcccac cggcagcagg tcgatacccg cctgcttttg
     4081 ttgatcccag tgacgggcac gtaattcacg cccaacagcc agcagttctt cacgcgtgga
     4141 gttccccgcc caataactct cttgcgcttt tttcagctcg cgacgcaggc caacgcgagg
     4201 gaaaccgagg gtgtgattaa gaattgtcat tatatgcccc ttatggaatt tttagtattt
     4261 agccgtccag atgtttacac atccataatt ggcggttact gtatattcct caagcgcaaa
     4321 ttattcatgc cgaagtgaag gactttcatg atcgaagtaa aacacctgaa aacgctacaa
     4381 gcgttgcgga actgcggctc actcgcagcc gctgcggcga cgttgcatca aacgcaatcc
     4441 gccctgtctc accagtttag cgatctggaa caacgccttg gcttccggct atttgtgcgt
     4501 aaaagccagc cgcttcggtt tacaccgcag ggagaaatcc tgttgcaact ggcgaatcag
     4561 gtgctgcctc aaatcagcca ggcactgcaa gcctgcaatg aaccgcagca gacgcgcctg
     4621 cgcattgcca ttgagtgcca tagctgtatt cagtggctga cgcccgcgtt agaaaatttc
     4681 cataagaact ggccgcaggt ggagatggat tttaaatcgg gcgtgacgtt tgacccgcag
     4741 cccgcgttgc agcagggtga gctggatctg gtgatgacct ccgatattct gccgcgcagt
     4801 ggcctgcatt attcgccgat gttcgattat gaagtgcgtc tggtgttggc acctgaccat
     4861 ccactggcgg cgaaaacgcg aatcacgccg gaagacctcg ccagcgaaac gctgttgatt
     4921 tatccggtgc agcgtagccg actggatgtc tggcggcatt ttcttcagcc cgcaggcgtt
     4981 agcccgtcac tgaaaagcgt cgataacacc ttgttgttga ttcagatggt tgccgcgcgg
     5041 atgggcatcg ctgcgctacc gcattgggtg gtggagagtt ttgaacgcca gggtctggtg
     5101 gtcaccaaaa ccctgggcga aggcttgtgg agccgactgt acgccgccgt gcgcgatggc
     5161 gagcagcgtc agccgattac agaagcgttt attcgttcag cgcgcaatca cgcctgcgat
     5221 catctgccgt ttgtgaagag cgcggagcga cccacttacg atgcacccac agtgaggcca
     5281 ggatcaccag cgcgcctgta ataaacgttg gccagtgcgg ctgttggtgc cagatagcca
     5341 ggttaaccaa cagccctgcc ggaacgtgca tattattcat aatgcccagc gttccggcgt
     5401 ccacctgagt cgcgccgtag ttccacataa agtagccaat accggaagcc accacgccaa
     5461 gaaacaccag aatgccccat tgcagcgtgg tttgcggcat tttctgcgca tttcccagca
     5521 aaaaccatgc aatcaccgcc accaggaacg caccaagata gaaccacgca aaggcgttat
     5581 gctgtggcat cggacgagtt tccatcaggc gtttgtaacc caccatgccg atggcaaaag
     5641 tgatattgga gagttgcacc agcagcaggc cagtccagaa gtggtcggtg acctgatcat
     5701 agcgaataat tccggcacca atcaccgcca acaaggcgct aaaagcatag ccccagcgca
     5761 gacggcgctt actcatgatg tcataaatca gcgtgatgta gagcggagtc agtacggtga
     5821 acagcaacag ttcggagacc gtcaggtaga gataggcgcg aaaactcagc atatacatca
     5881 caccgagctg catcgcgccc accagcatat acaggccaac cgttttcagg ctattgccac
     5941 gggtacgcag aaacggcaga aacacgagtg ccgccaggcc aacgcgcacc agcactgcaa
     6001 aatagctatc gacgtgcccc gcaaggtact cgccataaaa gctaaaggag aaggcccaca
     6061 gaatcgtggt gatgataagt agcgccacaa tggaaaactc tttgattaac gagtatccat
     6121 tgtagcgaag tatcaggttg acaactgacc aaataaagaa cgattacgat aaatagagtt
     6181 tacgcagata atgcggcacc gcgtcttcgg cattagtacc aatcacttcc agctccggat
     6241 gcagatcttt cagacgctgg tgcgcactgc ccataatgca gcctttacct gccatcgaca
     6301 gcatttcggc gtcgttcatc ccgtcaccaa aggcaataca atctttcaga ctgtagccca
     6361 gtttcttcgc caccgcttcc agggcatggc cttttgaaac gccgcccgcc atcacttcca
     6421 gacaagttag ggtagagaag ctgacgttga cgcgatcgcc ccaacgagcg ttgattgcct
     6481 gctccagcgg cagcagttgc tcatgagaat cgcaggtgaa gaacactttg ctgacgcctt
     6541 ccggctccag taatcctggc tcatacagcg catatttgaa cactgcttct ttaaaaaagc
     6601 gcatctcttc cgggcggtgg cgattcataa accattcgtc gtcgcgataa acgttagtaa
     6661 tgatgtccgg gttgtcgttg accacgccaa acagatcgct ggcaatgtcg cgatccaggt
     6721 tatgagcaaa aatcagattg ccatccagat cgtgaacacg cgcgccattg gaggtaatca
     6781 tgtaagactt aatctccaga ttatcgcgaa tttgccctac atcaacgtgg tggcgaccgg
     6841 tcgcaaacac aaagttaatg ccgcgcgcgg tgagcagctt cagagtttct ttggcgtaag
     6901 gggataacgt atggtcggga gaaagtaacg tgccatctaa atcagacgca acaacctggt
     6961 acataagaaa tttaaacctc tgtagagcgg tttccgctgg gtgagttatg cctgttgaaa
     7021 aaatcaacaa tggcgtggag cgcgactgag cgcattgcgt ccttttcaaa aaggatctca
     7081 tggtacgcac ctttaattac cagcggtcgt cccccttcga caggatggcc cgcagcggtg
     7141 cggagttcac aaaaacggtc gtgcatgcgg ttatccacca cgcgttcctc ttcagcctgc
     7201 aagagaagcg ttggcgtggc gtcatcaccc gcaccggcta acacctgttc gccagccaga
     7261 atactttcgc gtacccaatg gtaggtcggc ccaccgacgc gaatcgttgg gtcatcagca
     7321 taaaagcgta agttacgtcg atatcgctgt ctgctgtggg tcagtacgtt gatagcaaac
     7381 ggcaacgcgc gccagcgccc ggtgcctatt gcatagccat cacggaaacg tggatgcgct
     7441 tcggcccagt taaggatctg ccgcgccata aatgacggca tacgaatcac gatcccaaac
     7501 attggcgcag ttagcgcaat ggcgtcacat acacctggat ggcgttgcag aaataacgtg
     7561 gagatcgcac cgcccatcga atgtgccagt atatagcgct tacgccacgg accaggctgg
     7621 acctcctgtt gccagaatgc cgccaggtca tcgacataat cattaaagcg attaacatgc
     7681 ccaagatgcg gatcggctaa caggcgaccg gaacgtccct gcccgcgatg gtcgatgatt
     7741 aagacatcaa accccaaatg gaacaggtcg taggccagtt ccgcatattt tacgtagctc
     7801 tcaatacgcc ccgggcagat gactatcacc cggtcatggt gctgtgcgcg aaaacggaca
     7861 aagcgcaccg gaatgtcatc tacaccagta aactctgctt catcacgctg acgccagaaa
     7921 tcagtcagcg gtcccatggt aaaagcagca aacgcgtttt ctcttgtttc ccagtctttt
     7981 tgctgctgaa acatcgggta atctgcctct taaaccacgt aaaatcgttt ttttaagcgt
     8041 gcctgacaca acgcttcgac agtagcgtat tgtggcacaa aaatagacac accgggagtt
     8101 catcatgacc ttagaatggt ggtttgccta cctgctgaca tcgatcattt taagcctgtc
     8161 gccaggctct ggtgcaatca acaccatgac cacctcgctc aaccacggtt atcgcggcgc
     8221 ggtggcgtct attgctgggc ttcagaccgg actggcgatt catattgtgc tggttggcgt
     8281 ggggttgggg acgctatttt cccgctcagt gattgcgttt gaagtgttga agtgggcagg
     8341 cgcagcttac ttgatttggc ttgggatcca gcagtggcgc gccgctggcg caattgatct
     8401 taaaccgctg gcctctactc aatcgcgtcg acatttgttc cagcgcgcag tttttgtcaa
     8461 tctcaccaat cccaaaagta ttgtgtttct ggcggcgcta tttccgcaat tcatcatgcc
     8521 gcagcagcca caactgatgc agtatatcgt gctcggcgtc accactattg tggtcgatat
     8581 tattgtgatg atcggttacg ccacacttgc tcaacggatt gcgttatgga taaaagggcc
     8641 gaaacagatg aaggcattga ataagatttt tggctcgctg tttatgctgg taggagcgtt
     8701 gttggcatcg gcgaggcatg cgtgaaaaat aatgtcggat gcggcataaa cgccttatcc
     8761 gacttactct gatgaagcgt ttggcatcaa cgagaaataa tcaaatgaat gccaaatccg
     8821 gcaaataacg ccccggcaaa accatcaatc cacttcgcca gacgttgata accacggcgc
     8881 atttgcggta gagcaaacag gctggcaacc accgtaaacc atgccagtgt ttcgataata
     8941 atcaacgcga aaatgcccca gcgctcagtg gtgccgacgt tatcgccgac aaacagcgag
     9001 aataccgaac caaagtagat aattgctttc ggattggcga gattggtcag taagcctttc
     9061 aggaaactgc gcccgctttt tgccagttct acctgtggcg caggcgcaga aacgacctct
     9121 tttttcagtg cgctacgcag catctgataa cccatccagc agagatacag gccaccgccc
     9181 accataatca gcgtatgcag ccaggccatt ttttcgataa tcaaatgcag gccaagcaac
     9241 gcgatccctg cccagaccat tacgccgcag gtaatgccca gcacgcccat catcgcctct
     9301 ttacgggaac gactgacagc ggtctgagaa acaaagaaga aatcgggacc ggggctcata
     9361 agcgccacaa tgtgcaccat ggcaacggtg aggaataaca tcaacattca attgactcgc
     9421 ggagaaatcg tttacggagt cttcatcctg gcactttttt atgctgctga ctactcttcg
     9481 tcatcgccat cgacatgcgc acgaattagc gccataaacg gtttgccaaa gcgttccagc
     9541 ttgcgcatcc caacgccgtt aacgctgagc atttcgctgg cggagatcgg catctgttcc
     9601 gccatctcaa tcaaggtcgc atcgttaaac accacatacg gcgggacatt actttcatcg
     9661 gcaatcgatt tacgcagttt gcgtaatttg gcgaacagtt tgcgatcgta attgccaccg
     9721 aaagatttct gcattgcttt cggtttgagc gccacaatac gcggcacggc aagttgtaaa
     9781 gaggattcgc cgcgcaacac cgggcgtgcg gcttctgtca gttgtaatgc cgaatgctgg
     9841 gcgatgtttt gcgtcaccag accgagatga atcaactggc ggattacgct cacccagtgc
     9901 tcatgacttt tatcgcggcc catgccgtag actttcagtt tgtcatgacc atagtcgcgg
     9961 attcgctggt tattggcgcc gcgaatcact tccactacat agcccatccc aaaacgctga
    10021 ttcacacgac caatggtgga aagcgcaatc tgcgcatcgg ttgaaccatc gtattgtttc
    10081 ggcggatcga ggcagatatc gcagttcccg cacggctcct gccgcccttc gccaaagtag
    10141 ttcagcagca ccagacgacg gcaagtttgc gcttcggcaa aagcgcccat cgcattgagt
    10201 ttatggcgct cgatatcctg caactgcccc tgcggcttct cttccagaca acggcgcagc
    10261 cacgccatat cagccggatc gtaaaacagc atcgcttccg cgggcaggcc atcacgcccg
    10321 gcgcgaccgg tttcctgata ataggattcg atattgcgtg gaatatcaaa gtgaaccacg
    10381 aagcggacgt taggcttgtt gatgcccatg ccgaacgcta ccgtcgccac cacaatttgc
    10441 aggtcatcac gctggaattt ttcctgcacg tccgcacgga catcgttttc aagcccggcg
    10501 tgataagccg ctgcgctgat acccttgctt tgcagacgcg cggcggtgtc ttctactttc
    10561 gcacgactat tacagtaaat aatccccgac ttaccgcgtt gttcctgtac ataacgcatc
    10621 aactgatcga gcggtttgaa tttctccatc agcatgtagc gaatattcgg acggtcaaaa
    10681 ctgctaattt gaatcagcgg atcgttcagc cccagcaggc gcacgatatc ctgccgcgtg
    10741 gtgtcgtcgg cggtggcggt tagcgccata aacggcagcg tcgggaaccg ctggcgcaac
    10801 tgaccgagcg cggcatattc cgggcggaaa tcgtggcccc attgggagat acagtgcgct
    10861 tcatcaacgg ctaataacac cggattccag tgcgccagat gctcaagaaa gttatccagc
    10921 atcaggcgtt ccggggcgat ataaagcaga cgaatttgcc cggtgcgaca gcctgtcatc
    10981 acttcaagct gctgctcgcg ggtttgcgtc gagttaaggc acgccgccgc cacaccgttg
    11041 gcctgtagct gatccacctg atctttcatc aacgaaatca gtggtgaaac aaccacggta
    11101 agaccgttta gcagtaaggc aggaatttga tagcaaaggg attttccgcc accagtgggc
    11161 atgacgacca ggcaatcgcg accggaaagt acggtgtcga taatctcttc ctggccgggg
    11221 cgaaactgtt ggtagccaaa ggtttcttgt aaaacctgtt tagctccgga ctccagattc
    11281 aacacttccg cctgcgccac attaaccccg attgcctgaa ataaaaacag gcgctatttt
    11341 cagcgcctgc gcgagaaact gcaatgcttt tagtctctga gagggtaatc ctggatactg
    11401 gattccagtt ttgcctttca atcaactcac attagaacaa gtcgttcagc ataaccccca
    11461 cacccacacg ggtctggtta aagttatagt cgatgagcga ttcgccataa ccgctgtaaa
    11521 cctgggtata aaggcgcaca tgcctggtga tcgggtaact taagcctaac tccgcaccac
    11581 cgtaaccggt gttccagttg tactgtcctt tcgcactcag caccgcatca cccaggtgat
    11641 agccgatttt aagctggtag taacccatat atttggtgat atctgggtta tcgtcagtat
    11701 tccccaccac ataccacggc ttcacctcta ccagccagtt accgttttct gccatcagac
    11761 gggtataaag gcggttccag ctgcgggaag tcgggtctga acgcccgtta gagtcgtggt
    11821 tataacccat ctccacatcg cgcagcgtcc agccagcaaa gcggtaatcg gtggcaaaac
    11881 caaggaacaa ttgcggttcg tagttggttt cacgaaacgg tgaagactct tcgctattgg
    11941 acagttgcca ccaggatttt tgcgtgtaag aagcccccaa caccgagttt ggccccaata
    12001 ttccgcgcca cagcggaaat gccaggctca actggaattt cacttcatcc ttacgcgcgt
    12061 tttccgccca gtcataactg gcgatcgctt ctttgttcag atcgctggtt tgggtgtaga
    12121 tgaggtagtt ggtgtcataa ggatagagcg tgaaaggatt gtcatgctcc tgcagcatat
    12181 tcgcaataat actgccacgc accgcaggcg cgtcatgcac ctcttttacc gttgcctctt
    12241 gcgcatacac tgccattggc aacataaaca ccggcaacaa ccagccctgc agagtccgca
    12301 tcggtcgtgt tctccgtaac gaattattga ttagatgaat atttttctgc gggctatttt
    12361 acatacttaa ctcctttgcc gttagttatc ccttcctaaa gtagaaaacc acaattaaga
    12421 agcataaaat caacatttca ttaaccaatg aggtgtaagg atgatatgtc tgccgtactg
    12481 accgctgaac aagccctgaa attagtgggt gagatgtttg tttatcacat gccatttaac
    12541 cgcgcattgg ggatggaact ggagcgttac gaaaaagagt tcgcacagct ggcctttaaa
    12601 aatcagccaa tgatggtggg taactgggcg caaagcattt tgcacggcgg ggtcattgcg
    12661 tcggcgctgg atgtcgccgc aggtctggtg tgcgtgggaa gtaccttaac ccgccacgaa
    12721 accatcagtg aagatgaact acgccagcgg ctatcgcgga tggggaccat tgatcttcgc
    12781 gtcgattatc tgcgcccagg caggggcgag cgttttactg ctaccagtag tctgttgcgt
    12841 gcaggcaata aagtcgccgt tgcccgcgtt gaattacaca atgaagaaca gctttatata
    12901 gccagtgcca ccgccaccta tatggtaggt tgataacgta aaagcaggta aactgctttt
    12961 accaataaaa aatgagtttt gccaatggac accaaacaaa cgcggcaggg cgtattactc
    13021 gctcttgccg cttattttat ttggggtatt gcgccagcgt acttcaagct gatttactac
    13081 gtgcccgccg atgagatcct gacgcatcgc gtgatctggt cgtttttctt tatggtggtg
    13141 ctgatgagca tttgccgcca gtggtcctat ttaaaaacgc tgattcagac accaaagaaa
    13201 atcttcatgc tggcagtctc tgccgtgctg attggtggca actggctact gtttatctgg
    13261 gcggtgaaca atcaccatat gctggaagcg agccttggtt actttattaa cccgctggtg
    13321 aacattgtgc tggggatgat tttcctcggc gagcgattcc gccggatgca atggctggcg
    13381 gtgattctgg cgatatgtgg tgtattggtc cagctctgga cttttggttc gctgccaatc
    13441 atcgcgctgg ggctggcgtt tagttttgcc ttctacggcc tggtacgcaa gaaaattgcc
    13501 gttgaagcgc aaaccggcat gttaatcgaa accatgtggc tgctgcccgt ggcggcgatt
    13561 tacctgtttg ctattgccga cagctcaacc agccatatgg ggcaaaaccc gatgtcgctg
    13621 aatttactgc tgatcgccgc cggtattgtc accaccgtgc cactgttatg ttttaccgca
    13681 gcagctacac gcttacgact ctcaacatta ggctttttcc agtacattgg gccgacgcta
    13741 atgttcctgt tggcagtgac gttttatggt gaaaaaccgg gtgccgataa gatggtgact
    13801 ttcaccttta tttgggtggc gctggcaatt tttgtgatgg atgcgattta tacgcagcgt
    13861 agaacggcca aataaaaaat aaatatattc tgaaaaaata tgccgactct tgcatgagag
    13921 tgggcatatt gccatgctga caggagaccc acataaggaa agg
//
LOCUS       DAZKJR010000130        13644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKJR010000130 DAZKJR010000000
VERSION     DAZKJR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.131"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..1267
                     /locus_tag="ACHOWS_003995"
     CDS             122..1267
                     /locus_tag="ACHOWS_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFQ0049496.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPAPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITAKDAVNRATETRALLQSAYGRFVWFMLCVAGPGKDEAWAA
                     YVTRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRALNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            1269..1730
                     /locus_tag="ACHOWS_003996"
     CDS             1269..1730
                     /locus_tag="ACHOWS_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFQ0049497.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            1735..2040
                     /locus_tag="ACHOWS_003997"
     CDS             1735..2040
                     /locus_tag="ACHOWS_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFQ0049498.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            2030..2782
                     /locus_tag="ACHOWS_003998"
     CDS             2030..2782
                     /locus_tag="ACHOWS_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFQ0049499.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRASRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRND"
     gene            2782..3120
                     /locus_tag="ACHOWS_003999"
     CDS             2782..3120
                     /locus_tag="ACHOWS_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFQ0049500.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGKYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGVK"
     gene            3117..3503
                     /locus_tag="ACHOWS_004000"
     CDS             3117..3503
                     /locus_tag="ACHOWS_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049501.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDGR"
     gene            3603..3878
                     /locus_tag="ACHOWS_004001"
     CDS             3603..3878
                     /locus_tag="ACHOWS_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFQ0049502.1"
                     /translation="MTTKNTGTIILSVAGVTLSFEPNKTAFNNLINEITMTNKVAPMS
                     TYLNRIVTPASRDALADLIARYPGCEMQIAEKINEVYSPKLEIELKN"
     gene            complement(3842..4069)
                     /locus_tag="ACHOWS_004002"
     CDS             complement(3842..4069)
                     /locus_tag="ACHOWS_004002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049503.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
     gene            4068..6254
                     /locus_tag="ACHOWS_004003"
     CDS             4068..6254
                     /locus_tag="ACHOWS_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0049504.1"
                     /translation="MASQLDFIINLTDQLTRPLRQVQESVTGFAERSQGAFARIGVGV
                     AGLWGVAEGIRAAMDPAWEMQKALGELEAKGVGAQALTALTTQAMKLGTAFGQSSTEI
                     IRSAWHIRGALDGLSDGDLPRVTAATSTLAVAARVSSEEASGYLAAMGARFRSEFSAM
                     GAVGFAEDLAGKTAWMVQQFGVEMSTMQELIEGTKNAGADFGVSMAEQFAILGTLSRT
                     MGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRNIEGNVAA
                     QQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMTDPWDRLVAS
                     FYNIRAAIGNALYPILAPLAGRFADIGATFVRWLNMFPNIARWLGYITLTMLALGAAG
                     AVTNIVLGVFSFIMEGHKAIIKGARAAWSLYTLALTKVRSVIIATSLAARLAGVSFLT
                     MGAPVLLVVAAIAALVVVAIKFWQPVKAFIRGFIDGFTGALDAMSPVTAAFGDIARAL
                     APVWQGIKTLFGAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALGVVIS
                     VIRNLIDIFTIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTW
                     NWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETTVPALAGNRISAELPP
                     GGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG"
     gene            6251..6601
                     /locus_tag="ACHOWS_004004"
     CDS             6251..6601
                     /locus_tag="ACHOWS_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFQ0049505.1"
                     /translation="MNTPSLYIDLLITDGSFTLNSGNEPQRCDNRVSIGQDVVHSIIE
                     SGIAALLIGERSPTLRSDIITRLILLVEDDERLVPGTVFINEEAPGRLHVTAETQDFG
                     RINTRVNYERETGR"
     gene            6579..7763
                     /locus_tag="ACHOWS_004005"
     CDS             6579..7763
                     /locus_tag="ACHOWS_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFQ0049506.1"
                     /translation="MKGKPDVDFNQVFRESGMPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILTIVIRPVQWLRDALVDVVLPGMFVATADRSLLLLHAHDVNITPKPASKA
                     SGVIWFAKTRANDDVLIPEGTIVQTARINGVIYSMAVTEETLISSGKSGASVPVEATT
                     EGSGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSS
                     YHTDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYI
                     NEEGHHGHGDDLQCFAMPETRHRLDVTAYIRSSTTLPPDGAEGLKQDIGDLIRCAFRE
                     NARFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVV
                     IE"
     gene            7756..8304
                     /locus_tag="ACHOWS_004006"
     CDS             7756..8304
                     /locus_tag="ACHOWS_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0049507.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAARAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTEIYRRRISYAFINAQEAGEVAGFIAIFE
                     RLGIGYVELLERQPGVDWDVILVRVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     TPFIIHYGAYEGEYICWTARAE"
     gene            8327..10393
                     /locus_tag="ACHOWS_004007"
     CDS             8327..10393
                     /locus_tag="ACHOWS_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0049508.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLAAMDE
                     RQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLRAELTEDTPMVTPTSPSR
                     IWLNVWWTGTLTSEWTVEHEIIPADTDVTEERLRKTYPRDGIQQYFYAIAEIDEDGNV
                     TDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGYVGEAGQRWTTEQFVEWL
                     NERGAFRPGLWIFKANISLSRHSVLTDTGYGDICLGGCLIEVSGDINGGIIRITTGLE
                     SPNPADMYLRTQFTGVVLNKGKSTIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPW
                     PSDTPPAGYAIMVGQTFDKARYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQD
                     GIKSHTHGGSVSNTDLGTKTTSSFDYGTKTTSTNGQHEHSVPLRRQDGGGINFDWLDG
                     SSSGSHIGNGYVPAAGDHQHTVPIGAHTHTLALGSHGHGLTINPTGNAENTVKNIAFN
                     YIVRLA"
     gene            10393..10989
                     /locus_tag="ACHOWS_004008"
     CDS             10393..10989
                     /locus_tag="ACHOWS_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFQ0049509.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENTWQLMEDHRGKTVWRLESGSALLITESGPLPENVTIIKP
                     DGEFCRWDGSSWAKDEAAEQAYRVSEAENKKRELIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLSRTDTGNAPDIVWPESPSPDHHAG"
     gene            11001..11726
                     /locus_tag="ACHOWS_004009"
     CDS             11001..11726
                     /locus_tag="ACHOWS_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049510.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQRMAQAA
                     ASLSTDKMQIPITTDTLPAPVHLSVATMRSAVSAARAAQARQLAAQRPDAAGLRRQIS
                     RFISRRAGALAGLEEGIRTLAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAA
                     LLAADSLTELGKMIHEPDRTARP"
     gene            11698..12240
                     /locus_tag="ACHOWS_004010"
     CDS             11698..12240
                     /locus_tag="ACHOWS_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049511.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARATPASSRKQTPQTPGAKGGTVADET
                     PEKMTWFEEKVLKPVNDALE"
     gene            12243..>13644
                     /locus_tag="ACHOWS_004011"
     CDS             12243..>13644
                     /locus_tag="ACHOWS_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049512.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLPKLARVEAPTEAVSAGN
                     IADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLPVPMAGSESGMFQFPPPGTLVEVG
                     FVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRDGVSQRVTVAGDWERQTDQRIQET
                     SRERVVSADEEKRSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENYSISTGGHMM
                     VVAGTCERDVEGDTQDT"
BASE COUNT         3191 a         3967 c         3974 g         2512 t
ORIGIN      
        1 cgtcgcgcgt ctttctgggg gtaacaaacg aagaattttc acacatcctg gcacgccaga
       61 tgaacgcaat gggctacggc ctggacgttg acgcacagga tatcagataa ggagtcacac
      121 cgtgagctgg ccgaatgtaa ccgtaagcca tcagaaccgt ttcaacggtg ccacccgtga
      181 agtggagcgc acgctgcttt ttgtgggcta tggcaaaaag aacacaggta acaccctttc
      241 cgtctcccct gagaccgacc tggacgacgt gctgggaccg gatgaaagcc tgcttaaatc
      301 cacgcttacc gccgcaatcg ccaacggtgg ccagaactgg tttgcctatg ttcacgttct
      361 gtctgaaccc aaacccccgg caccggaggg cggcgacgct aacgccgcat gggtggatgc
      421 ggtgaaaaaa gcgcagacca tcgcctctgc cgaaggcgtg gtgattgcca ttgacatcac
      481 ggccaaagat gccgttaacc gcgccaccga aacccgcgcc ctgcttcagt ccgcttatgg
      541 ccgttttgtc tggttcatgc tgtgcgtggc cggaccgggc aaggatgagg catgggcggc
      601 atacgtcacc cgcatcagtg ccatacagga cggcgtggca gcacctggcg tgatggtggt
      661 tccccgctta tggggaaatg agcccggcgt actggcgggc cgtctctgta atccttccgt
      721 caccgttgcg gacagtccgg cacgtgtggc aaccggtgcc ctggtggcga tgggtaacga
      781 tgagatcccg caggacggca agaaacagcc ccttgagctg gccacgctgc gctcgctgga
      841 aggcaaacgc ttcagcgtgc ccatgtggta tcacgatttc gacgggctct actggtcaga
      901 cggacgcacc cttgatgtgg agggtggcga ttaccaggtc atcgaaaacg tgcgcgtggt
      961 ggataaggtc tcccgccgtg tacgtctgcg cgccattgcc cgccttgcag atcgcgcgct
     1021 caacagcaca ccgggcagca ttgccgccag ccagacatat ttcagccgca cgctgcgtga
     1081 aatggcgctc gccacggaga ttaacggcgt accgttcccc ggggaggtta aaccgccaca
     1141 ggacggggat atcaccatcg tctggaccag cagtacacac gtgcagattt acattgtggt
     1201 acgcccctac gagagcgcaa aagccattgg cgtaagtatt gaactcgaca cagcactgga
     1261 gatctgacat ggccggaaat gaacgtatca gcggcggcgc gtttgatatc aactacgacg
     1321 ggatcatgat ccacgtggaa agcgccacag tcaccatcac cgacaacagc gccgtggcac
     1381 agacacgcgg cgtgccgaac ggctacaccc gtggttccgt ttctgccgat gtggaggtgg
     1441 aagtcgacgc gcagaacttt aaaaagtttg gtgctgtggc ccgctctgcg gggtcatggc
     1501 gtgccattcc tgaaaaggat tttctctttt atgccaacaa cggcagcgag gaggaaaaaa
     1561 tcgaggtgtt cggctgcgtc cccatgttgt cggacgtagt gaacatcagc gcaggtgaag
     1621 ccggaaagac cacgaaaaaa atcaagttca tggtcacaag cccggacttt atcagcattg
     1681 acggcgtgcc ctacctgtca gcccgtgaca cccgcgatct gaaggggtga tgccatgccg
     1741 gacggtcaga cttcactcct ggcaaaactg attatcatcg gtgctgccat cgggctgggg
     1801 cgtctcatgg tgagccgcga tcgcatcaca gcccgtctgc ttatcgggcg aatgattatg
     1861 ggctctgccg tggcaccggt tgccgccatt ccgctgttta aatttccgga catgccggag
     1921 ctggtagtgg tcggtctggc gtgcgcgctg ggcattatgg gcagcgcctt tattgaagaa
     1981 tgcttttgcc gtctggcaga ccgctacctg aagcagaagg aacgcagaaa tgacccataa
     2041 gccttacatt tcgccttccc tgagcctgta tcacgcgcag gcgcatgacg ttctgcggat
     2101 gctgccggaa aacagtgtgg acgcgctcat cacagacccg ccatacagtt caggtgccac
     2161 acacaaggcg gggcggacat gccagggcag ccacgccaaa tacatcaacg atgaagatct
     2221 tcaccgcttt gaaggcttcg aaggcgagaa catggacgcc cgttcatggg aatactggac
     2281 gcatctgtgg atgacacagg catggcgcac ggtacgaccc ggtggttacg ccctggtctt
     2341 caccgactgg cggcagttgc ccgcgctgac ggacgcattc caggccgccg gctttacctg
     2401 gcgggggatc gtggcatgga acaagggacg ggcctcaaga acaccgcata ccggttattt
     2461 tcgtcaccag tgcgaatatg tggtctgggg cagtaaaggc gcgctgggca aatccccttc
     2521 cgggccgttc gacggctgca tcacgcacca cacacgccca caggagaaac agcacccgac
     2581 aggtaaaccg gaatcgttga tggatgagct tgtgcgcgtg tgtgaacccg gagggctgat
     2641 cctcgatccg tttatggggt ccggcaccac gggcgtggcc gcccttcgcc ggggctgccg
     2701 ttttacgggt attgaactga acgcccacta tttcagcgtg gcacaggcgc gccttacggg
     2761 gacggaacag aggaatgact gatgaaactg agcgaaaaac aacaactgtt tgccgtcatg
     2821 attgccgatc tcatccactg ggcacaggag catggctatc gcctgacctt cggcgaggca
     2881 taccgcacgc cggagcaggc cgcactgaat gcgaaaagcg gaaaaggcat tcgcaacagc
     2941 cttcacacgc tgcgcctcgc ggtggacttc aacctgttca ttaacggcaa ataccaggcc
     3001 gataccgacg cctacaggcc gctgggggaa tactgggaat ccatcggcgg cacatggggc
     3061 gggcgcttca gccgggcaga cggcaatcat ttcagccttg agcacaacgg ggtgaaatga
     3121 tgaaacagct ggcgggctgc ctggtgatcc tctttgccgc catgtgggcg ggctggctgg
     3181 cccatgactg gcatgacgcc agggtggcgc ttgccgaaca ggaagccatg gaagccacgc
     3241 gacgtgcggt aacagaagcc gggcagcagt ccgcccgcat actggaaaaa cggctggagg
     3301 cgttaaaggg caatgaaatt cacactgaac gcgttattca cacggaaacc gtccgcccgg
     3361 tattccgcaa tgtgtgcgct actgatgatt acgtccggct gttcaacgcc cgtgctgaag
     3421 ccgctgagcg taccctggca ggaaaaccag ctgacggaat gcccgaagac actgccgaga
     3481 ctggcaggcc cgacgggcgc tgattttgat gcggcactgc gaaccctcac gagccgctac
     3541 accgaatgcg ccgccaggca taacagcctg gtggaaacca tacgaaacac ggagaaactg
     3601 caatgaccac gaagaacacc ggcaccatca tcctgtccgt tgcgggcgta accctgagct
     3661 ttgaaccgaa caaaacggca tttaacaacc tcatcaacga aatcacgatg accaacaagg
     3721 tggcgccgat gagcacctat cttaaccgca tcgtgacgcc tgccagccgc gacgcactgg
     3781 ctgacctgat tgcgcgttat ccgggctgtg agatgcagat cgccgagaag attaacgagg
     3841 tttactcccc gaaactggag attgagttaa aaaactgacc gcgcgggtcg aaagcatccg
     3901 ccgcaatccc cttgagcagt atctggcatt gcggcgtcac tacctccccg gagagccgga
     3961 cgacgaagag agcatcgccc gtgccatctg gctggatgaa tattttgccc gtacccgcgc
     4021 caacaagacc gctgaaggta tagcgatcgc atttaacgga aggtgacatg gccagccagc
     4081 tggatttcat tatcaatctg acagaccagc ttacccgccc gctgcggcag gttcaggaat
     4141 ccgtgacggg attcgccgaa cgctcgcagg gcgcgttcgc gcgtatcggt gtgggtgtgg
     4201 ccggactgtg gggcgtggcc gaaggtatcc gcgccgcgat ggatccggcg tgggagatgc
     4261 agaaggcgct gggcgagctt gaggccaaag gtgtcggggc gcaggcgctg accgccctca
     4321 ccacgcaggc catgaaactg gggaccgcat tcggtcagtc ttccacggag atcatccgtt
     4381 ccgcatggca tattcgcggc gcactggacg ggctgagcga cggggattta ccccgtgtga
     4441 cggcagcaac cagtaccctg gccgtggctg cccgcgtcag cagcgaagag gcgagcggct
     4501 accttgccgc catgggtgcc cgtttccgca gtgagttttc tgccatgggt gccgtggggt
     4561 ttgctgaaga tctggcaggt aaaaccgcgt ggatggttca gcagttcggc gtggaaatga
     4621 gcaccatgca ggagcttatt gagggcacca aaaacgcggg cgcggatttt ggcgtcagca
     4681 tggcagagca gtttgccata ctgggcaccc tgtcacggac catgggcacc gaggcatcgg
     4741 gcatctatga ggaattcctg cgcagtgccc ccgctgccgc acaaaaaatg gggatgagtt
     4801 ttgttgatgc caccgggaag attttaccca tgggtgacat cctcgaccgt ctgcaggcga
     4861 ggttcggcag gaacatcgag ggtaacgtgg cggcacaaca ggcgctggat gacgccttcg
     4921 gcagtggcgc ggacgtgatt aaaaaactct ggggacagca ggaaatgctt aaccggagca
     4981 tatcagcgct ggggcgtaat gacggcatga agcgtgccac ggaaatggcg gaagccatga
     5041 ctgacccgtg ggaccgcctt gtggcctcct tctacaacat ccgggccgcc atcggtaacg
     5101 ccctctaccc cattctcgca ccactggctg gccgttttgc cgatatcggc gcgacgttcg
     5161 tgcgctggct gaacatgttc ccgaacatcg cccgctggct gggttacatc acgctgacca
     5221 tgctggcgct gggcgcagcc ggtgcggtca cgaacatcgt gctgggtgtg ttcagcttca
     5281 tcatggaagg ccataaggcc atcataaagg gcgccagggc agcctggagt ctgtacacgc
     5341 tcgcactcac aaaagtacgc agcgtcatca ttgccaccag tcttgccgcg cgtctggcgg
     5401 gcgtgtcgtt tctgaccatg ggcgccccgg tcctcctggt tgttgccgcc atcgccgctc
     5461 tggtggtggt ggccattaag ttctggcagc ccgtcaaagc ctttattcgc ggctttattg
     5521 atggttttac cggcgcactg gacgccatga gcccggtgac tgccgcgttc ggcgacattg
     5581 cccgggcgct ggccccggta tggcagggca taaaaaccct gttcggtgcc ttctccgatc
     5641 tgtttgcacc ggtacagctg accacgggac agatgaacag cgtcacccgt gccggggaaa
     5701 catgcggtcg cgtggtggcc gcagcggtaa gcctgatatc ccccgcgctg ggggtggtta
     5761 tcagcgtgat cagaaacctg atcgacatct tcaccatcgt catcagaggg tggcaggacg
     5821 tggtggctgc atttgacccg accgccccgg tcgaatcctt ccggaaaatg gcggggatca
     5881 tcggtaacgt cttcggggac ttatggaacg tcattaaaaa ctcgtttaac cggacgtgga
     5941 actggcttgc ggaaaagctg aacaaaatcc ccggtatcag tatcgacatg aagcccacgg
     6001 agcaggtgaa caccatgacg cggggcgcaa cgggcagcac cgtacctccg cagattgcag
     6061 aaaccaccgt cccggcactg gcaggcaacc gcatcagcgc agagctgcca cccggaggca
     6121 tacgcaacca gatacgcacg gacacctcaa cccgtatcga caacagccgg acgttcggca
     6181 atctgacaat caacacgcag aacccgatga cgccggggca actggatgaa tggacggagc
     6241 tttacgcggg atgaacacgc catcgcttta catcgacctg cttatcactg acggcagttt
     6301 tacgctgaac agcggaaacg aaccacagcg atgtgataac cgggtcagca tcggccagga
     6361 cgtggtgcac agcattatcg aaagcgggat cgccgccctg cttatcggcg aacgcagccc
     6421 gacgctgcgc agcgacatca ttacccggct tatcctcctg gttgaggatg atgaacggct
     6481 ggttccgggg acggtgttca ttaacgaaga ggcaccgggc aggctgcatg tgacggcaga
     6541 gacacaggat tttggacgca taaacaccag ggtgaactat gaaagggaaa ccggacgttg
     6601 attttaatca ggtattccgt gaaagcggta tgccggaaac agaagaagag atcgccaccc
     6661 tctttcatga ggatgtacag aaaaccggac tcatcaccaa cacctcgccc atgagtccct
     6721 tctggcggct cattctgacc attgtcatac gacccgttca gtggctgcgt gatgcgctgg
     6781 ttgacgttgt gctgcccggt atgtttgtcg ccacggctga ccggtcactt cttctgcttc
     6841 atgcgcacga cgtcaacatc accccaaaac cggccagtaa ggccagtggc gtgatctggt
     6901 ttgccaaaac cagggcaaat gatgacgtcc taatccccga aggcaccatc gtccagaccg
     6961 ccagaattaa cggcgtgata tacagcatgg ccgtcacgga agaaacgctt atcagcagcg
     7021 gtaaaagcgg cgcatccgtt cccgtggaag ccaccaccga aggcagcggg cacaatctcg
     7081 cgccggggta ttttcgtatc cttcccgttg ccattcccgg cattgcatcc gtggtgaacc
     7141 gggaggactg gctgaccgta ccgggtgccg atgaggaaag cgacgacgaa ctgcgcgaca
     7201 gaatacgcaa ccagtttaac ctcgtgagca gttaccacac ggacgccgtc taccggggac
     7261 ttatcgccag cgtggcgggc ctgagcatcg accggatcta ctttcagcat gacgccccga
     7321 gaggaccggg aacggccaac gcctacctgt tactggacag cggcgaggcc tctgcccctt
     7381 tcattgaagc agtcaaccgc tacatcaacg aagaggggca ccacggacac ggggatgatt
     7441 tgcagtgttt cgccatgccg gagacgcgtc accgtctgga cgtcaccgca tacatcagaa
     7501 gttccaccac cctgccaccg gacggcgcgg aagggctgaa acaggatatc ggcgacctga
     7561 tacgctgcgc tttccgggag aacgcccgct ttaatgtgaa aaaagtctgg ccctattcgc
     7621 gcttttcgtt ttccacgctg ggcaaggaaa ttcatcagac cttcccggtg gtggcttcgg
     7681 ttcagttttc actggaagac atcatcagcg atctggccgt gccacggctc gaatccctga
     7741 cagtggaggt ggtcattgaa tgactttttg cagcggcttc gctcgctgaa actgccctca
     7801 tggatggaca ggggagaacc cgacaaactg ctgcgggccg cccgggcgtt ctggtcccgc
     7861 gtatgggaat ggatcacctg gcccgtgcgc cagtttgacc ccatgacatg tgcagagcct
     7921 gtacttgatt tgctggcatg ggagcgggac atcacccgct ttaaagggga acccacggag
     7981 atctaccgcc gccgcatcag ttacgccttc atcaatgcac aggaggctgg cgaggtggcg
     8041 ggctttatcg ccatttttga acggctgggc atcggctacg tggagttact cgagcgccag
     8101 cccggcgtgg actgggatgt gatcctggtg cgggtgacag acagccagat agccgacaac
     8161 agcgatcttc ttctggagat catccgcaaa tatggcagga catgccgccg ttaccgtttt
     8221 gaagtggaaa ccctcacccc cttcatcatc cattatggcg catacgaagg ggagtatatc
     8281 tgctggacgg ccagggcaga gtgaatgact gaaggagtat gactttatgg cgaaatctgt
     8341 aattaccagc gcctttatcc ggctcaaggc acagcaggcg gcggaaggca cccccgttgt
     8401 gctggatgag tttatttttg caaacgtccc ggacctcaac ccggaaacgc cggtatcccc
     8461 cgacgaaacc ctgccgcctg aagatcagat tgttcaccgc caggccgttg gtaaaaccgg
     8521 cgtggtgaat gagaatgccg tggtttattc ggtcaccctg ggggcagagc tgggtgattt
     8581 tgaatttaac tgggtgggac tggtcagcaa agcaaccagc acgctggcca tgattgttca
     8641 cgcccccaca cagaaaaaaa tcagaaccca caacggtaac gaaggtaatg tgctggtccg
     8701 ttccatgctc atggagtaca gcggtgcgaa ggaagccaca ggtatcacca ccccggcgca
     8761 gacctggcag atagacttca cggcacgcct ggcagccatg gacgaacgcc agcgccggga
     8821 aaacgtcgat atctacggga aggcaacttt ttttgatgac gggtggctgg ttaagaaagg
     8881 cgaaacctgg acagtcaaca aaggggtggg ttacgtggag gggctacgcg cagagctgac
     8941 cgaagacaca ccaatggtca cgcccacctc cccgtcccgt atctggctga atgtgtggtg
     9001 gaccggtacg ctcaccagtg aatggacggt ggagcacgaa atcataccag ccgacacgga
     9061 cgttacggag gagcgtttac ggaaaacata tcccagggac ggcattcagc aatattttta
     9121 tgccatcgct gaaattgatg aagacggcaa cgtgactgac ctgcgcccca gaggaacccc
     9181 ggaagagcgc cgcagtctgg acgccctgaa aaaacacgag aaatcacgca accacccgga
     9241 cgcaacgctg aaagaaaagg gcttcacaca gttaagcagc gccaccacca gtacatcaga
     9301 gacacaggca gccacgccaa aggctataaa gatcgccatg gacaacgccg atggtcggct
     9361 tgcgaaaaac cgcaatggtg aggacatccc caacaaagag ctgttcctga tcaacgtggg
     9421 cgcaccacgc gtttatgcaa aagacgggta cgtgggtgaa gcagggcaac gctggacaac
     9481 agaacaattc gtggaatggc tgaatgagcg tggcgccttc agaccaggac tctggatatt
     9541 caaagccaat atttcacttt cccggcacag cgtgctcact gatacgggct atggtgatat
     9601 ctgccttgga ggctgcctta tcgaagtatc aggcgacatc aacgggggga tcatcaggat
     9661 cacaacaggt ctcgaaagcc caaaccctgc cgacatgtac ctccgcacac agttcaccgg
     9721 ggttgtttta aataaaggga aaagcaccat ctggtaccgc acttacacgg acaaaaacaa
     9781 acccacacca caggacatcg gcgcactgcc tgcaagtgag ttgttacccg caggcgttcc
     9841 cctgccgtgg ccgtcagaca ccccgcctgc cggttacgcc atcatggtgg ggcagacatt
     9901 cgataaagca cgatatcccc tgctggcaaa agcctatccg gatggtcgta tcccggatat
     9961 gcgtggctgg acaatcaagg gaaaacctgc cagcggtcgt gcggtattgt cacaggaaca
    10021 ggacggcatc aaatcccaca cccacggggg gagtgtctca aatactgacc ttggcacgaa
    10081 aacgacctca tcatttgact acggaaccaa aacaacctct acaaacggac aacatgaaca
    10141 cagcgtcccc ctgagaagac aagatggcgg aggcataaac tttgactggc tggatggttc
    10201 atcgtccggt tctcatattg gtaatggata cgttcctgcc gcaggtgacc accaacatac
    10261 cgtacccatt ggcgcacaca cccacacgct cgcactgggt tcacatggtc acggcctgac
    10321 cattaacccc accggtaatg ccgaaaacac cgttaaaaat attgcattta actacatcgt
    10381 gaggcttgca taatgacctt taaaatgagc gaacacccac agacaattaa aatctacaac
    10441 ctgtcagccg gaacaaatga atttatcggc gaaggcgatg cctggatccc tccgcatacc
    10501 ggactgcctg cgcactcaac ggacattgcc ccgcctgaca ttcctgaagg acacgtggct
    10561 atctttgatg aggatgaaaa cacctggcag ctcatggaag atcaccgggg taaaaccgtc
    10621 tggcgtctgg agtcgggaag cgccttactg attactgagt cgggcccgtt accggaaaac
    10681 gtgaccataa taaaaccgga cggggagttc tgccgctggg acgggtcatc atgggctaag
    10741 gatgaagcgg cagaacaggc ttaccgggtg agcgaggcgg agaataaaaa acgggaatta
    10801 atacaggaag caacaaatac aataaacatc cttcaggatg ccgtggattt aaatatggcg
    10861 accgacgatg aaagcgccct gcttcccctg tggaaaaaat accgggtatt actgagccgg
    10921 acagacacgg gcaacgcgcc ggatattgtc tggcctgaat ccccttcgcc ggatcatcac
    10981 gcaggctgac cggaggcacc atgacgtgga caagaaaaac aatcagcgtc agcgctgaca
    11041 tgcccccgct gagctgttcc gtcatccccg tcacaccgtg gacttacggg ctgggacgat
    11101 atgaagaatc gggggtatat ttaagccctc cgaacgccat aaactggctg gccgggaaaa
    11161 tggccggaag ccgtgcatcc ggcgacgtga ccattatcat gattgccgaa aacacccacg
    11221 atctttttat acagtcaatg gcggcactga ccgccgttct gcccgtgccg gtattcacgc
    11281 aggcacagcg catggcacag gctgccgcca gtctgagcac tgacaaaatg caaatcccga
    11341 tcaccaccga caccctgccc gcacctgtgc atctttccgt tgccacaatg cgcagcgcag
    11401 tcagtgccgc ccgtgcagca caggccaggc agctggcagc acaacgcccg gatgccgcag
    11461 ggcttcgccg ccagataagc cgctttatca gcagacgggc tggcgcactt gccggactgg
    11521 aagaggggat cagaacactg gcagaacaga aggcgcaggc ctgggtattt cagtaccggg
    11581 ggtatcaccg cgccgcagcg gcggccatgg tgaaggatat cccggcaccg accgccgtac
    11641 ataccgttgc cgcactcctg gcggcagact cattaactga actggggaaa atgatccatg
    11701 agccagaccg cactgctcgc ccttgatggt gagggtattt tcatgcagaa catggtgatt
    11761 tcgccctcga tgcagttcca ggaaaaggac cagtccggcc agacatcgag caccgcgaac
    11821 gccgagcagg gcatcaaggc caaagagctt cgcgtaaccg ggatgatcac ctttgacaat
    11881 gagcgggccc ttcagcgcat tttccagctg gcctctgcca ccaccgggga cggtgccctg
    11941 aaagtttacc gtatcgcaaa cgccaccgct gccgccatta actttcgtga agggacattt
    12001 tccgggcaga tagacgcaca gcagcagacc gaccgcctcg cctggcaggt cagcttcaca
    12061 ttacgcgaaa gacgaagcgt accggaaaaa cgccaggcac gcgccacccc cgccagcagc
    12121 cgtaaacaga cgccgcagac gcccggcgca aaaggcggga ccgtggccga cgaaacaccg
    12181 gagaaaatga cctggttcga agaaaaggtc ctcaaacccg tcaatgatgc gctggagtaa
    12241 ccatgaaccc cactaaacgt ctgttactgg gtaacgacac cattcagctg gtcagttgtt
    12301 ccgtcatgct tgagctgaac gcgtgcggac gcggatttat cacagccaga acagagcaga
    12361 actacaccgg gcgccccgtt cgtctcgata tcggatacgg aaacgacctg gtgcgctggt
    12421 ttaccggata cgttgaacgc agccagcccg ctgaaaacgg ctcggtgcgg cttctgattc
    12481 gtgaaatggc ggggatcctt gatcacccct tcccctgttc atttcagcac ccgacaatgc
    12541 gcacggtcac acagtggctg agtgagacca gcgggctgga aatcatcctg ccggacaacg
    12601 cggcttacac tgacagaccc gttccgcatt tcacacacag cggcacgggc tacgaactgc
    12661 tggccagtct ggggcgtatt ttctccgtac cggattacat ctggcatccg ttaccggacg
    12721 gcggcattat ggccgctgct gccgcgcagg ggatgttttc cggtcgcccc gtcaccatcc
    12781 cgcacgagtt cagccaggca tcaacgggcg ggcacagtat gaccctcccg atgattcaga
    12841 cgctgcgacc gggtgtggag gtgaacggtc agcgcctgac gatggtcatg ctggaagatg
    12901 acaacatgac catcacgtgg acaccacgca ataaagccac cggcgccccc ctccagaagt
    12961 cccctttccg tcgccaggct gaaaaggcct tccctgaact ggcatcaggg cttcacctgc
    13021 ccaaactggc acgtgttgag gcaccgaccg aagccgtcag cgcgggcaat attgccgacc
    13081 cgttcagacc ccgctacgcg gtgaatctcc agctgcttga cgagaacggc aacccggcag
    13141 cagatacgcc cgtctataac gccgttcccc tccccgttcc catggcgggc agtgagtccg
    13201 gcatgttcca gttcccgcca cccggaaccc tggtggaagt gggatttgtg gaaggccggg
    13261 cggataagcc cttcatccgc cagataatgg cagagggcca caatctgccc gacgtgaagc
    13321 cgggcgagca gttgcagcaa cagcgcgacg gcgtatcgca gcgcgtgacg gtcgccggag
    13381 actgggagcg ccagacagac cagcggatac aggagacatc acgcgagcgc gtggtgagcg
    13441 cagacgagga aaagcggagc ctgaccagcc gggagaccac catacaggca accgacacaa
    13501 ccaccgtcct gggcgtatcc acgcttcagg ctggcgcggt cacccatatc actgaagaga
    13561 actacagcat cagcacgggc gggcacatga tggtggttgc cggtacctgt gagcgcgacg
    13621 tggaaggcga cacccaggac accg
//
LOCUS       DAZKJR010000131        13601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKJR010000131 DAZKJR010000000
VERSION     DAZKJR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.132"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            219..1328
                     /gene="livK"
                     /locus_tag="ACHOWS_004012"
     CDS             219..1328
                     /gene="livK"
                     /locus_tag="ACHOWS_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFQ0049513.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1376..2302
                     /gene="livH"
                     /locus_tag="ACHOWS_004013"
     CDS             1376..2302
                     /gene="livH"
                     /locus_tag="ACHOWS_004013"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFQ0049514.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2299..3576
                     /gene="livM"
                     /locus_tag="ACHOWS_004014"
     CDS             2299..3576
                     /gene="livM"
                     /locus_tag="ACHOWS_004014"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFQ0049515.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGSFYAIGAYTFALLSHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3573..4340
                     /gene="livG"
                     /locus_tag="ACHOWS_004015"
     CDS             3573..4340
                     /gene="livG"
                     /locus_tag="ACHOWS_004015"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFQ0049516.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4342..5055
                     /gene="livF"
                     /locus_tag="ACHOWS_004016"
     CDS             4342..5055
                     /gene="livF"
                     /locus_tag="ACHOWS_004016"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFQ0049517.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5143..5364
                     /locus_tag="ACHOWS_004017"
     CDS             5143..5364
                     /locus_tag="ACHOWS_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0049518.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            5361..5738
                     /locus_tag="ACHOWS_004018"
     CDS             5361..5738
                     /locus_tag="ACHOWS_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0049519.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            5947..7263
                     /gene="ugpB"
                     /locus_tag="ACHOWS_004019"
     CDS             5947..7263
                     /gene="ugpB"
                     /locus_tag="ACHOWS_004019"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFQ0049520.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            7439..8326
                     /gene="ugpA"
                     /locus_tag="ACHOWS_004020"
     CDS             7439..8326
                     /gene="ugpA"
                     /locus_tag="ACHOWS_004020"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFQ0049521.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            8323..9168
                     /gene="ugpE"
                     /locus_tag="ACHOWS_004021"
     CDS             8323..9168
                     /gene="ugpE"
                     /locus_tag="ACHOWS_004021"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFQ0049522.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9170..10240
                     /gene="ugpC"
                     /locus_tag="ACHOWS_004022"
     CDS             9170..10240
                     /gene="ugpC"
                     /locus_tag="ACHOWS_004022"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFQ0049523.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLKGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVLRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10237..10980
                     /gene="ugpQ"
                     /locus_tag="ACHOWS_004023"
     CDS             10237..10980
                     /gene="ugpQ"
                     /locus_tag="ACHOWS_004023"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0049524.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10967..11407)
                     /gene="yhhA"
                     /locus_tag="ACHOWS_004024"
     CDS             complement(10967..11407)
                     /gene="yhhA"
                     /locus_tag="ACHOWS_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFQ0049525.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11527..13272
                     /gene="ggt"
                     /locus_tag="ACHOWS_004025"
     CDS             11527..13272
                     /gene="ggt"
                     /locus_tag="ACHOWS_004025"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFQ0049526.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            complement(13310..13594)
                     /gene="yrhB"
                     /locus_tag="ACHOWS_004026"
     CDS             complement(13310..13594)
                     /gene="yrhB"
                     /locus_tag="ACHOWS_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFQ0049527.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFIQGWYFCFESQEFL
                     ESGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         3041 a         3418 c         3893 g         3249 t
ORIGIN      
        1 gacaacggcg cttttttctg tttatctatc aataaattca gaatattatc tgttcttaat
       61 cgactgaaaa atagggattt taatcgctat catcacaaaa tactgtgcta aacccttaat
      121 cagacaggca aaaacagtgc agtataaaaa aagaacagtc tgatttgtta acacataaga
      181 acaaagcaac acaacatcac gaatggggat ttttgactat gaaacggaat gcgaaaacta
      241 tcatcgcagg gatgattgca ctggcaattt cacacaccgc tatggctgac gatattaaag
      301 tcgccgttgt cggcgcgatg tccggcccga ttgcccagtg gggcgatatg gaatttaacg
      361 gcgcgcgtca ggcgattaaa gacattaatg ccaaaggggg aattaaaggc gataagctgg
      421 ttggcgtgga atatgacgac gcctgcgacc cgaaacaagc cgttgcggtc gccaacaaaa
      481 tcgttaacga cggcattaaa tacgttattg ggcacctgtg ttcctcctct acccaacctg
      541 catcagatat ctacgaagac gaaggtattc tgatgatttc gccgggagcg accaatccgg
      601 agctgaccca acgcggttat caatacatca tgcgtactgc cggtctggac tcttcccagg
      661 ggccaacggc ggcaaaatac attcttgaga cggtgaagcc ccagcgcatc gccatcattc
      721 acgacaaaca acagtatggc gaagggctgg cgcgttcggt gcaggacgga ttgaaagcag
      781 ccaatgccaa cgtcgtcttc ttcgacggca ttaccgctgg tgagaaagat ttctccgcgc
      841 tgatcgcccg cctgaaaaaa gaaaacatcg acttcgttta ctacggcggt tactacccgg
      901 aaatggggca gatgctgcgc caggcccgtt ccgttggcct gaaaactcag tttatggggc
      961 cggaaggggt gggtaatgcg tcgctgtcga acattgctgg tgatgccgcc gaaggtatgt
     1021 tggtcactat gccaaaacgc tatgaccagg acccggcaaa caaaggcatc gttgatgcgc
     1081 tgaaagcaga caagaaagat ccgtccggac cttatgtctg gatcacctac gcggcggtgc
     1141 aatctctggc gactgccctt gagcgtaccg gcagcgatga gccgctggcg ctggtgaaag
     1201 atttaaaagc taacggtgca aacaccgtga ttgggccgct gaactgggat gaaaaaggcg
     1261 atcttaaggg atttgatttt ggtgtcttcc agtggcacgc cgacggttca tcgacggcag
     1321 ccaagtgatc atcccaccgc ccgtgaaatg cgggcggttt tagaaaggtt accttatgtc
     1381 tgagcagttt ttgtatttct tgcagcagat gtttaacggc gtcacgctgg gcagtaccta
     1441 cgcgctgata gccatcggct acaccatggt ttacggcatt atcggcatga tcaacttcgc
     1501 ccacggcgag gtttatatga ttggtagcta tgtctccttt atgatcatcg ccgcactgat
     1561 gatgatgggc attgataccg gctggctgct ggtggcggcg ggattcgtcg gcgcaatcgt
     1621 cattgccagc gcctacggct ggagtatcga acgggtagcc tatcgtccgg tgcgtaactc
     1681 taagcgcctg attgcgctta tttccgcaat cggcatgtct atcttcctgc aaaactacgt
     1741 cagcctgacc gaaggttcgc gcgacgtggc gctgcccagc ctgtttaacg gtcagtgggt
     1801 ggtgggccat agcgaaaact tctctgcctc tattaccacc atgcaggcgg tgatatggat
     1861 tgttactttc ctcgccatgc tggcgctgac gattttcatt cgctattccc gtatggggcg
     1921 cgcctgccgt gcctgcgcgg aagatctgaa aatggcgagc ctgcttggca tcaacaccga
     1981 ccgggtgatt gcgctgacct ttgtgattgg cgcggcgatg gcggcagtgg cgggcgtgct
     2041 gctcggtcag ttctacggcg taattaaccc ttacatcggc tttatggccg ggatgaaagc
     2101 ctttaccgct gcggtgctcg gtgggattgg cagcattccg ggggcgatga ttggcgggct
     2161 gatcctgggg attgcggagg cgctctcttc tgcctatctg agtacggaat ataaagatgt
     2221 ggtctcattc gccctgctga tcctggtgct gctggtgatg ccgaccggta ttctgggtcg
     2281 cccggaggta gagaaagtat gaaaccgatg catattgcaa tggcgctgct ctctgccgct
     2341 atgttctttg tgctggcggg cgtctttatg ggcgtgcaac tggagctgga tggcaccaaa
     2401 ctggtggtcg acacggcttc agagatccgt tggcagtggg tgtttatcgg cacggcggtg
     2461 gtctttttct tccagcttgt gcggcctgct ttccagaaag ggctaaaaag cgtttccggg
     2521 ccgaagttta ttctgcccgc gattgatggc tccacggtga agcagaaact gttcctcgtg
     2581 gcgctgctgg tgcttgcagt ggcgtggccg tttatggtgt cgcgtgggac ggtggatatc
     2641 gccactctga ccatgatcta cattattctc ggtctcgggc tgaacgtggt tgttggtctt
     2701 tctggtctgc tggtgctggg atacggcagc ttttacgcca tcggcgctta cacttttgcg
     2761 ctgctcagtc actattacgg tctgggcttc tggacctgtc tgccgattgc cgggttgatg
     2821 gcggcggcgg cgggcttcct gctcggtttt ccggtgctgc gtctgcgcgg tgactatctg
     2881 gcgatcgtta ccctcgggtt cggcgaaatt gtgcgcatat tgctgctcaa taacaccgaa
     2941 attaccggcg gaccgaacgg tatcagtcag attccgaaac cgacgctctt cggactcgag
     3001 ttcagccgta ccgcccgcga aggcggctgg gacacgttca gtaatttctt tggcctgaaa
     3061 tacgatccct ccgatcgcgt catcttcctc tacctggtgg cgttgctgct ggtggtgtta
     3121 agcctgtttg tcattaaccg cctgctgcgg atgccgctgg gccgtgcgtg ggaagcgttg
     3181 cgtgaagacg agatcgcctg ccgttcgctg ggcttaagcc cgcgtcgtat caagctgact
     3241 gcctttacca tcagcgccgc gtttgctggt tttgccggaa cgctgtttgc tgcgcgtcag
     3301 ggctttgtca gcccggaatc cttcactttt gccgaatcgg cgtttgtgct ggcgatagtg
     3361 gtgctcggtg gtatgggttc gcaatttgcg gtgattctgg cggcaatttt gctggtggtg
     3421 tcgcgagagt tgatgcgtga tttcaacgaa tacagcatgt taatgctcgg tggtttgatg
     3481 gtgctgatga tgatctggcg tccgcagggc ttgctgccca tgacgcgccc gcaactcaag
     3541 ctgaaaaacg gcgcagcgaa aggagagcag gcatgagtca gccattatta tctgttaacg
     3601 gcctgatgat gcgcttcggc ggcctgctgg cggtgaataa cgtcaatctt gaactgtacc
     3661 cgcaggagat cgtctcgtta atcggcccta acggtgccgg aaaaaccacg gtttttaact
     3721 gcctgaccgg tttctacaaa cccaccggtg gcactatttt actgcgcgat cagcatctgg
     3781 aaggtttgcc cggccagcaa atcgcccgca tgggcgtggt gcgcaccttc cagcatgtgc
     3841 gtctgtttcg tgaaatgacg gtgattgaaa acttgctggt ggcgcagcat cagcaactga
     3901 aaaccgggct gttctccggc ctgttgaaaa cgccttcttt ccgccgcgcc cagagcgaag
     3961 cgctcgaccg tgccgcaacc tggcttgagc gcattggttt gctcgaacac gctaaccgtc
     4021 aggcgagtaa cctggcctac ggtgaccagc gccgtctgga gatcgcccgc tgcatggtaa
     4081 cgcagccgga gattttaatg ctcgacgaac cggcagcagg tcttaacccg aaagagacga
     4141 aagagctgga tgagctgatt gccgaactgc gtaaccatca caacaccact atcttgttga
     4201 ttgaacacga tatgaagctg gtgatgggga tttcagacag aatttacgtg gtcaatcagg
     4261 ggacgccgct ggcaaacggt acgccggagc agatccgtaa taacccggac gtgatccgtg
     4321 cctatttagg tgaggcataa gatggaaaaa gtcatgttgt cctttgacaa agttagcgcc
     4381 cactacggca aaatccaggc gctgcatgag gtgagcctgc atatcaatca gggcgaaatt
     4441 gtcacgctga ttggcgcgaa cggggcgggg aaaaccacct tgctcggcac gttatgcggc
     4501 gacccacgcg ccaccagcgg gcgaattgtg tttgatgata aagacattac cgactggcag
     4561 acggcgaaaa tcatgcgcga agcggtggcg attgtcccgg aaggacgtcg cgtcttctca
     4621 cgcatgacgg tggaagagaa tctggcgatg ggcggctttt ttgctgaacg cgaccagttc
     4681 caggagcgca taaagtgggt gtatgaactg tttccacgtc tgcatgaacg ccgtgttcag
     4741 cgggcaggca ccatgtccgg cggtgaacag cagatgctgg cgattggtcg cgctttgatg
     4801 agtaacccac gtttgttatt gcttgatgaa ccctcgctcg gactggcacc gattatcatc
     4861 cagcaaattt tcgacaccat cgagcaactg cgcgagcagg ggatgaccat cttcctcgtc
     4921 gagcagaacg ccaaccaggc gctgaagctc gctgatcgcg gctacgtgct ggaaaacggt
     4981 catgtggtgc tctccgatac tggcgatgcg ctgctggcga acgaagcggt gagaagtgcg
     5041 tatttaggcg ggtaataata ttttgagtaa aaaggagtcg aatgactcct tttttgtact
     5101 tgttagttgt acaatttaat tgtacttaat atggaggata tcatgcatac catgaactac
     5161 agcgaagcgc ggcagaacct tgcattagtt ctggaaacag cggcgacagg tactccggta
     5221 acgatcacga ggcgtgggca taaggcagct gtgattatca gcgcagaaga gtttgaacgt
     5281 taccagaagg ctaaactgga cgcagaattt gacgccatca tggctattca tggcagcgaa
     5341 attaaggagt tagcggataa atgaccattc agtttatctc agcggaggag ataatttatt
     5401 tccacgataa attgcttagc gttacaccag gtgtaacagg aatgtccgat ccagggcgag
     5461 cagaagcgct tttgtaccgg gtactgaata agtatgagta tgaaggcgtt tctgatcttt
     5521 ggctgctggc ggcgatgcat ctattggcaa tctcgcgcgg tcatatcttt aatgatggta
     5581 ataagcgcac cgcactgttt atcacgctat tattccttaa acgtaacggc atcatacttg
     5641 ctgcaaaccc agagtttgta gaattgacgg ttgatgtggc ggcgggaaaa gtatcgttag
     5701 aagatatcgt tacccgcttg cgtcaacgcg gtacctgaca aggttcttag ccattctgct
     5761 ttttatccgt gccccatctc ccattttccc tcacccacgc cgtcaccgcc ttgtcatctt
     5821 tctgacacct tactatctta caaatgtaac aaaaaagtta tttttctgta attcgagcat
     5881 gtcatgttac cccgcgagca taaaaagcgt gaattcgcgc atccggtaca acaagagaga
     5941 taaacgatga aaccgttaca ttatacagct tcagcactgg cgctcggact ggcgttaatg
     6001 gggaatgcac aggcagtgac gaccattccg ttctggcatt ctatggaagg ggaactgggt
     6061 aaagaggtgg attctctggc ccaacgtttt aacgccgaaa acccggatta caaaattgta
     6121 ccgacctata aaggcaacta cgaacagaat ttaagcgcgg ggattgccgc atttcgtacc
     6181 ggcaacgcgc cggctatttt gcaggtttat gaagttggca ccgccaccat gatggcgtcg
     6241 aaagccatta aaccggtata tgacgtgttt aaagaggcgg ggattcagtt cgatgagtcg
     6301 cagtttgtgc cgacggtttc aggctactac tccgacagca aaaccggcca cttactctcc
     6361 cagccgttca atagctcgac tcctgttctc tattacaaca aagacgcctt caagaaagcc
     6421 ggtttagacc cggaacagcc gccgaaaacc tggcaggatc tggcggacta tgccgcgaaa
     6481 ctgaaagcct ccggtatgaa gtgcggctac gccagcggct ggcagggctg gatccaactg
     6541 gaaaacttta gcgcctggaa cggtctgccg tttgccagca aaaacaacgg ctttgacggc
     6601 acagacgcgg tgctggagtt caacaagccg gagcaggtga aacacatcgc tatgctcgaa
     6661 gagatgaaca agaagggcga tttcagctac gtcgggcgta aggatgaatc cactgagaag
     6721 ttctataacg gtgattgcgc gatgaccacc gcctcttccg gttcgcttgc caacattcgt
     6781 gagtacgcca aatttaacta cggcgtaggc atgatgcctt acgacgccga tgcgaaagat
     6841 gcaccgcaaa acgccattat cggcggggcc agcctgtggg tgatgcaggg caaagataaa
     6901 gaaacgtata ccggcgtggc gaagttcctc gacttcctcg cgaagccaga aaacgctgcc
     6961 gagtggcatc agaaaaccgg ttatctgcca atcaccaaag cggcgtatga cctgacccgt
     7021 gagcagggct tttatgagaa aaacccaggg gcggataccg cgacgcgtca gatgctgaat
     7081 aagccgccgt taccgttcac caaagggctg cgtctgggca acatgccgca gatccgcgtg
     7141 attgtggatg aagagctgga gagcgtgtgg accggtaaga agacaccgca gcaggcgctg
     7201 gataccgccg ttgagcgtgg gaaccagtta ctgcgtcgct ttgagcagtc tacgaagtct
     7261 taattctctt accctcaccc taaccctctc ccaaagggag aggggaccga tcggcgtggt
     7321 atcctggtgc cgtgtcggtt tgcaacttcg ctcggtggga aagtatgcac cgcctttctc
     7381 tctcgcccct ctggggagag ggccggggtg aggggaacac aacacgaaga gtttaactat
     7441 gtcatcatcc cgtccggtgt tccgctcgcg ctggctgccc tatttgctgg tcgcgccgca
     7501 gctcatcatc accgttatct tttttatctg gcctgcgggc gaagcgttgt ggtactcgct
     7561 gcaaagcgtc gatccgtttg gtttctccag ccagtttgtc ggcctggata acttcgtcac
     7621 gctgtttcat gacagctact atctcgactc cttctggacg acgataaaat tcagcacctt
     7681 tgtcaccgtc agcggtttgc tggtgtcgct gttctttgcg gcgctggtgg agtacatcgt
     7741 gcgcggcagc cgtttctatc aaaccttaat gctgctgcct tacgctgtgg ctcccgccgt
     7801 tgccgccgta ttgtggatct tcctgtttaa ccccggtcgc gggctgatca cccattttct
     7861 cgctgagttc ggctacgact ggaaccacgc gcaaaacagc ggtcaggcga tgtttctggt
     7921 ggtgtttgcc tcggtgtgga agcaaatcag ctacaacttt ctgttcttct atgccgcgct
     7981 gcaatccatt ccccgttcgt tgatcgaagc cgcagccatc gacggtgcag gcccgattcg
     8041 ccgcttcttt aagattgcgc tgccgcttat cgctccggtg agtttcttcc tgctggtggt
     8101 gaacctggtg tacgccttct tcgatacctt cccggtgatc gacgccgcca cgtccggcgg
     8161 gccggttcag gccaccacga cgctgattta taagatttac cgcgaaggct ttaccggact
     8221 ggatctggct tcgtctgccg cgcagtcggt ggtgttgatg ttcctcgtca tcgtgctgac
     8281 ggtggtgcag ttccgctatg ttgaaagcaa ggtgcgttac caatgattga gaaccgtccg
     8341 tggctgacaa tattcagcca taccatgctg atcctcggga tcgcggtgat cctcttcccg
     8401 ctgtacgtgg cgtttgtcgc ggcgacactg gataaacaag ccgtctacgc cgcgccgatg
     8461 acgctaatcc ccggcacgca tctgctggaa aacatccaca acatctgggt gaacggggta
     8521 ggcacgaata gcgcgccgtt ctggcggatg ttgcttaaca gctttgtgat ggcgttcagc
     8581 attacgctcg gcaaaattac cgtctcgatg ctctcggcct ttgccattgt ctggtttcgt
     8641 tttccactac gtaacctctt cttctggatg atttttatca ccctgatgct gccggttgaa
     8701 gtgcgtatct tcccgacggt ggaagtcatc gccaacctga aaatgctcga cagctacgca
     8761 ggtttaacgc tgccgctgat ggcctcggcg accgccacgt ttctgtttcg ccagttcttt
     8821 atgaccctac cggatgaact ggtggaagcc gcgcggatcg acggcgcgtc accaatgcgt
     8881 ttcttttgcg acatcgtttt tccgctgtcc aaaaccaatc tggcggcgct gtttgtgatc
     8941 acctttatct acggctggaa ccagtatttg tggccgttgt tgattattac cgacgttgat
     9001 ctcggcacca ccgtggcagg gattaaaggg atgatcgcca caggcgaagg cacaacggaa
     9061 tggaactcgg tgatggcggc gatgttgtta accctgatcc caccagtagt gattgtttta
     9121 gtgatgcagc gtgccttcgt gcgcggcctg gtcgatagtg agaaataaga tggcaggact
     9181 gaaattacag gcagtaacca aaagctggga tggcaaaacc caggtcatta aaccgctgac
     9241 ccttgatgtg gcggatggcg aatttatcgt gatggtcggg ccgtctggct gcgggaaatc
     9301 gacgttattg cgaatggttg ccggactgga gcgggtaacg acgggcgata tctggatcga
     9361 ccgcaagcgt gtcaccgaaa tggagccgaa agatcgcggg attgcgatgg tgttccagaa
     9421 ctacgcgctt tatccgcata tgagcgtcga agaaaacatg gcgtgggggc tgaaaattcg
     9481 cggcatgggc aagcagcaaa ttgccgagcg cgttaaagaa gcggcgcgca ttctggagct
     9541 ggacggcctg cttaagcgcc gcccgcgtga gctatctggc ggtcagcgtc agcgtgtggc
     9601 gatggggcgt gcgattgtgc gtgagccggc agtgttcctg tttgatgagc cgctctctaa
     9661 cctcgatgcc aagttgcgtg tgcagatgcg tcttgaactg caacagctgc accgtcgcct
     9721 gaaaacgact tcactctacg ttacccacga tcaggttgag gcgatgacgc tcgcccagcg
     9781 agtaatggtg atgaacggcg gcgttgccga acagattggc acgccagttg aagtgtacga
     9841 aaagcccgcc agcctgtttg tggcgagttt tatcggcagt ccggcgatga atctgctgaa
     9901 agggcgtgtg aataacgaag gtacgcattt cgaactggac ggcggtatcg cgctgccgct
     9961 aaacggtggc taccgtcagt atgctggacg taaaatgact ctcggcattc gcccggaaca
    10021 tattacgcta cgttcgcagg ctgaaggtgg cgtgccgctg gtgatggaca cgctggagat
    10081 cctcggcgca gataacctgg cgcacggacg ctggggcgaa cagaagctgg tgttgcgact
    10141 ggcgcatcag gagcgcccga cggcaggcag cacgctgtgg ctgcatctgc cggaaaatca
    10201 gctacatctt tttgatggtg aaacaggaca acgagtatga gtaactggcc ttatccccgc
    10261 atcgtcgccc atcgtggcgg cggtaaactg gccccggaaa acaccctggc ggcaatcgat
    10321 gtgggggcaa gatacggtca taagatgatc gaatttgacg cgaagttatc gaaagatggc
    10381 gagatcttct tgcttcatga cgacaatctc gaacgcacca gcaacggctg gggcgtcgcg
    10441 ggtgaactga actggcagga tttactgcgc gtggatgcgg gcagttggta cagcaaagcg
    10501 tttaaaggtg agccgctgcc gttgctttcg caggtggcgg aacgctgtcg cgaacacggg
    10561 atgatggcga atatcgaaat caaacccacc actggcaccg ggccattaac gggcaaaacg
    10621 gtggcgctgg cggcacgcga actgtggacc ggtatgacgc cgccgctgct gtcatcgttt
    10681 gagattgatg ctttagaagc cgcgcaacag gcagcaccgg aactgccgcg tggtttattg
    10741 cttgatgaat ggcgcgacga ctggcgagaa ttgaccgcgc ggctgggctg cgtctctatt
    10801 catctcaatc ataagttgct cgataaagcg cgagtgatgc agttgaaaga cgccggatta
    10861 cggattctgg tttataccgt caataaaccc cagcgcgcgg cagagttgct gcgctggggc
    10921 gtggattgca tctgtaccga tgcgattgac gtgattggtc cgaactttac ggcccaatag
    10981 ttttcaacgg aatgtcaggc tgcggcgtgc tgggctgatt cagcatgtcg ccgtttctcc
    11041 tttccggcag catatgctgc tgattcaacg aactatccgg attacggttg ctgtttaaca
    11101 tcccgccatt ggtgttgggc agcatttgct gtcgggcggg atttcgctcc cccggctgtg
    11161 actgcaacac ccgctgagaa ttattgttta tctggttttc taaatgctgc tgttgcagct
    11221 gcgtttgcgt tttcagttgc tgattcagca tccctttttg ctggatttgc tgcgtttgca
    11281 tttgggtttg catccgctgc tgactgggga tctgataccc cggctggtta gggttattca
    11341 gagtattaat gggctgtgca aagccgacaa acggcaggag tgccgtaaga atcagaagtc
    11401 gtttcatcgc gtatcctcct ctgaagatat tctttaagtt tactcctttc ctgcaatcat
    11461 caggattaat tcagagttat ataccaggct tagctggggt tgccccttaa tctctggaga
    11521 ataacgatga taaaaccgac gtttttacgc cgggtggcca ttgctgctct gttcacagga
    11581 agttgtttta gtacggtagc cgcgccacct gcgccgtctc ctgtttctta cggcgtggag
    11641 gaagatgtct ttcatccggt acgcgcgaaa caggggatgg tggcgtctgt agatgctact
    11701 gccactcagg tgggggtgga tatcctcaag gagggcggga atgccgttga tgccgccgtg
    11761 gctgtgggct acgcgctggc ggtaacgcat ccgcaggcag ggaatctggg cggtggtggt
    11821 tttatgctga tccgctcgaa aaatggcaat accaccgcta tcgatttccg cgaaatggct
    11881 cccgccaaag cgacccgcga tatgttcctt gatgatcagg gtaacccgga cagcaaaaaa
    11941 tcactcactt cgcatctggc ttctggtacg cccggtacgg tagcaggttt ctcgctggcg
    12001 ttggataaat acggcaccat gccgttgaac aaagtggtgc aaccggcgtt taaactggca
    12061 cgcgatggtt ttatcgttaa cgacgcgttg gctgacgatc tcaaaaccta cggtagcgaa
    12121 gtgttgccga atcacgaaaa cagcaaggct atcttctgga aagagggcgc gccgctgaaa
    12181 aagggcgaca agctggtgca ggcgaacctg gcaaagagcc tggagatgat tgccgaaaac
    12241 ggcccggacg aattctacaa aggcacgata gcggaacaga ttgcccagga gatgcagaaa
    12301 aacggtggct tgatcactaa agaagattta gcggcgtata aagcggtcga acgtacaccg
    12361 attagcggcg aatatcgcgg gtatcaggtt tactcgatgc caccgccatc ttccggtggg
    12421 attcatatcg tgcagatcct caatatcctg gaaaacttcg atatgcagaa atacggcttt
    12481 ggcagcgccg atgcgatgca aatcatggca gaagcggaga aatacgccta cgccgaccgc
    12541 tcggaatatc ttggcgatcc ggattttgtc aaagtcccgt ggcaggcgct gaccaataaa
    12601 gcctatgcca aatctattgc cgatcaaatc gatatcaata aagcgaagcc atccagcgaa
    12661 attcgccctg gcaagcttgc gccttatgag agtaatcaaa ctacccatta ctcagtggtg
    12721 gataaagatg gtaacgcggt ggcggtgacc tatacgctga acaccacctt cggtacgggc
    12781 attgtcgcgg gcgagagcgg tattctgctt aataaccaaa tggatgattt ctccgccaaa
    12841 ccgggcgtgc cgaacgttta cgggctggtg ggtggtgacg ctaacgcggt cgggccgaat
    12901 aaacgtccgc tgtcgtcgat gtcaccaacc attgtggtga aagacggtaa aacctggctg
    12961 gtcaccggaa gcccaggcgg tagccggatc atcactaccg tgctgcaaat ggtggtgaat
    13021 agcatcgatt atggtatgaa tgtcgccgaa gcgaccaatg caccgcgttt ccaccatcag
    13081 tggttgccgg atgagctgcg tgtcgagaaa ggatttagcc cggatacgct caagctgctg
    13141 gaagcaaaag gtcagaaagt ggcgctgaaa gaggcgatgg gcagtacaca aagcattatg
    13201 gttgggcctg acggtgagtt gtacggcgca tccgacccgc gctcagtaga tgatttaacg
    13261 gcggggtatt aaggttagcg gccctcttcg tgggaagagg gccgtattgt cagggcaagc
    13321 cgaaggtagc cttttttatt tcataatcct gtagatattc ttccagcggc tttgccgttc
    13381 caagggaatg aatttcacca ctgtctttat caataataaa aggtgcgtta ccggctaagc
    13441 gcgcggcctc atcaccagat tcaagaaatt cttgtgattc gaaacagaaa taccagccct
    13501 ggataaaacg tccatgcaga gtgatgacga cagggagatc tgcatcatca aggtaatggt
    13561 tcgctttcgc gaatgcgtcg tgataagtaa tcataattat a
//
LOCUS       DAZKJR010000132        13587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKJR010000132 DAZKJR010000000
VERSION     DAZKJR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.133"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..219)
                     /locus_tag="ACHOWS_004027"
     CDS             complement(<1..219)
                     /locus_tag="ACHOWS_004027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049528.1"
                     /translation="MEKYVKSVAIGKPCVQCTSRSAPLEASLRENHIDMKMRKGDRQY
                     YLNKEGDTFRLVKRVKTFSKSATLGKTKA"
     gene            complement(308..2569)
                     /locus_tag="ACHOWS_004028"
     CDS             complement(308..2569)
                     /locus_tag="ACHOWS_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFQ0049529.1"
                     /translation="MRLWIAEKPELGKLIAQVLGNPQYFDGYIVCNNDTVSWAIGHLL
                     KLIPPELKTPEYKSWSKNTLPLQLRPLQYIVIPKTEKQFNVLSGLITRADMIIHAGDP
                     DEEGQLLIEEILSYCGNKAPVKRVLINDLNKDAVRQAMRNLQDNRAFYGLYQKALARS
                     AGDYLFGLNMTRAYTIAAREKGHNEILSVGRVQTPVLGLVVRRYEENRDYKKYDYWCV
                     QAEFIDTDTIISPMLVPPEDAPVDDKGRIISEQYCDEVIRSCKGQQASVSLAESEIKS
                     RRPPLPFSLLDLQVKMNRQYGFTAEKVLEITQKLREEYRAITYNRSDCRYLSEEQFSQ
                     SPATLAMLSSFFPELGKIFSHIDSGRKSRAFNDKKISAHTAIIPTPTPTGLDQMNEDE
                     MRVYKAIVAQYLVQFMPDKTFDSAVVEFNINGFLFRQRAIRPVSPGWSILLADRDEET
                     LPDCKLFDAITSLTKGQQLVCSSVNSRKETSTPPPLYTEASLLEDLRRVARYVEDPRI
                     RQLLLDRDEGKEEERGGIGTPATRGHILNVLQVRGFIAVKNKKVVPTELGLSFIHALP
                     EVMTRPDMTALWHEQQSMIETGEISIDDFLDELEQFILQQVSDVDISGLAVAEYQCTC
                     GGRYVRRRTDKGFFWGCSKYPDCRNAVPDRGGVPDFSRSEFERKVRCPVCGSGMKISP
                     KAYNCSNERCGFRLWGTQFGKELTLAQATDLLMKGHTREIKGLKKKDGAKFDAVLNLN
                     RDGSVVPTFKKKK"
     gene            complement(2610..2843)
                     /locus_tag="ACHOWS_004029"
     CDS             complement(2610..2843)
                     /locus_tag="ACHOWS_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFQ0049530.1"
                     /translation="MENKYRKYPKSSKQEYLFQLRKCSKLSTLDKIIQRKAYTMNDID
                     FVEFLTAADHRLAEIVTGQLFDKIPKNVWKLIY"
     gene            complement(2893..3249)
                     /gene="ssb"
                     /locus_tag="ACHOWS_004030"
                     /pseudo
     CDS             complement(2893..3249)
                     /gene="ssb"
                     /locus_tag="ACHOWS_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(3261..4526)
                     /locus_tag="ACHOWS_004031"
     CDS             complement(3261..4526)
                     /locus_tag="ACHOWS_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0049531.1"
                     /translation="MGKLTLVEQALRLHKEYYGDECFGTIVKNYDYYPKVAEIFINRK
                     NKNTYSNLDIEPVASSGVVHTGSNPVMVKVGRTKKLMFKDGSGAFRIHLGKNEVVHIV
                     RYIHNSSIRMEYAIGTTKGLFMLHNLCEAGRVKLNRSLPRKGIWKIGDYQGIYYYGKS
                     RKEEIENALRFSGHPKFSELENDFNQFFSDIDYYTRYGQSGMRKILFTGPPGTGKTTI
                     AKALGAKYQDKYVFVYADNYFREVCYAAANKKIPLIIIAEEADELYRADAGTLSFLDG
                     ADTPRNPAGTYVIFSTNYPRRIDPRIRKRPGRIDRVISIGAFRTKDAASCAKMYLPEG
                     ININIKELGAVLDRTTPAEIKEIINISIGMIRGTKKELSTEIIKNARAFLKGSLDLSI
                     QEAEEDIDEREDIYRQNGAQPDYLSYLED"
     gene            complement(4544..5056)
                     /locus_tag="ACHOWS_004032"
     CDS             complement(4544..5056)
                     /locus_tag="ACHOWS_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFQ0049532.1"
                     /translation="MKITLTIFIFLLTAFQAANAGGIPGALAGILSTLNQEIQGKVIR
                     VLDGDTIEVLQDKKPVRIRLANIDAPEKKQAFGRWSANQLKALLAAQPVTVTYTQTDR
                     YGRIIGRVFTTNGTDASRFMVQSGAAWVYERYNTDKSLPALQREAQEQKRGLWADTNP
                     VPPWEWRKNN"
     gene            complement(5135..5710)
                     /locus_tag="ACHOWS_004033"
     CDS             complement(5135..5710)
                     /locus_tag="ACHOWS_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0049533.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKEYPVKELPDIDESAHPF
                     YVCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTHMDCHSIAYYDIHTLPLISCGR
                     QKIVPFAALIKADRCIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCEHR
                     FRQPVGEDNWKQAISEECAIRYANRLIQCEG"
     gene            complement(5827..6033)
                     /locus_tag="ACHOWS_004034"
     CDS             complement(5827..6033)
                     /locus_tag="ACHOWS_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049534.1"
                     /translation="MSEIRFVTLSSDTDINKVIQEGLTQKDATTTIVLGCASEGKEAP
                     LRLIETCFRTIPKHISIEEINEIK"
     gene            complement(6046..6435)
                     /locus_tag="ACHOWS_004035"
     CDS             complement(6046..6435)
                     /locus_tag="ACHOWS_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049535.1"
                     /translation="MDKSDEEKIGYILDEHERIFRKQASLYTVLGILNLIICLLCVVA
                     TFCVSVILPESHLKHLLFSFTILCMVCSFIIYYYTRHMVLNRQELLRLMRLTEGAPDV
                     KQELLNRLLSGKILTYWDEKDIRRLIF"
     gene            complement(6563..6874)
                     /locus_tag="ACHOWS_004036"
     CDS             complement(6563..6874)
                     /locus_tag="ACHOWS_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFQ0049536.1"
                     /translation="MKYSVIAVIILFLLSSKSYAEEVSIDSGDPCTVFMCMAGKVYGE
                     NSSECKGPTKKFFNIIKKKKGRIRWSKTFDARKAFLMNCPGADPAHVSKIMSKFGRKL
                     F"
     gene            complement(6899..8527)
                     /locus_tag="ACHOWS_004037"
     CDS             complement(6899..8527)
                     /locus_tag="ACHOWS_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFQ0049537.1"
                     /translation="MGKQKIPDGWVLENGKLIQKRVTHYPPIIFGKDPYSNNLLVSYG
                     QTYLMLAAPPGSGKGVGIVIPNLLLYPHSVVVNDCKFENWDLTAGFRKACGQKVVRFS
                     PERLETHHWNPLKVINQDPLYRLGDVRTLASSLYVPDNMKNASWFSKAADVFTAIVLY
                     LIETPSLPLSLPQCYEIASLGTRIGEWAQRVIQARVGTPDALSEETVRELGVIISESK
                     GREFSTLMSFVTGRLSLYGEKLVALALTESDNEEENIDFSKLREEPTSIYFCVTEGAL
                     QKFGPLMNLFFSQAIRENSKILPEQGGNLEDGSLRLKYQVLFLEDEFAVMGRIKVMET
                     APALSRGAGLRYIIVFQNKSQVCSDDCYGPAGGKAVMDPFHIEVVFAPGDISTAEEYS
                     KRLGTYTERVASSSRNISEGQRRSRGRNVTLHARPLMLPQEVNELPYPEELVFMQPTK
                     WNPALKGRVKKIMWYKEPELTARVDPVRYPVPDIPAGNISKLKGLVVPISQYITDAVK
                     ISSPEGDYLNEEQKKRLNIPQVESERESYEDNDY"
     gene            complement(8527..8889)
                     /locus_tag="ACHOWS_004038"
     CDS             complement(8527..8889)
                     /locus_tag="ACHOWS_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049538.1"
                     /translation="MEKLKLFIVIIALCCLFFVIGLYAGGWMWLELSGVDSSPSLMTL
                     ISQGGLPALNDRARMLLPWAWCVTVAITFFPAGVSLLAMTHGADGNRKDLHGNARFAG
                     KRELRRFWYEGPEKEDRN"
     gene            complement(8901..9950)
                     /gene="virB11"
                     /locus_tag="ACHOWS_004039"
     CDS             complement(8901..9950)
                     /gene="virB11"
                     /locus_tag="ACHOWS_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFQ0049539.1"
                     /translation="MNTEFDLDKSELVLGWLDQSGVTERLSRQGVTEVAINRPFEIWT
                     ETSKDGWQREDAPWLTLNMCEHLAETLAVYNNRILGVKSPIKTVELPGGERGQIVIPP
                     ACEKSTVSMTFRKPSLQRFSLENYDSSGRFDNANRVRGRSKELLDWQKELKRLNAARE
                     YVEFFRLAVRHKLNIIIFGGTGSGKTTFAKAIVDVYPSNRRLLTIEELNEMKLPYHLN
                     HVHLLYGDYVTPKDLVACCMRMKGDHILLAELTGDETWDFMEVLNTGHPGTVTTAHAN
                     SSLHGYARISGLIKQSAVGAGLDISYIDRRVRTSFDVVMYMEKTEILDITYEPEVQLA
                     LLNGASMESALSFAS"
     gene            complement(10161..11516)
                     /locus_tag="ACHOWS_004040"
     CDS             complement(10161..11516)
                     /locus_tag="ACHOWS_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFQ0049540.1"
                     /translation="MTENVVTPPENETKKETSAADENKPQGQGVGAFSLKPAGRKKPV
                     NWKIVGVIIGGIVIVLLFTIIFAAIVINNRADPEPEIDETGIKAEVVTRSIGVIDDPM
                     GAFMEAHLPQDRDKTESDSRTVQSSGKQESNGPVSDKKNTQTSGGQSTVNPVTPVTDI
                     RVPEMLFSPVARFDSSGISQGVGGSSDGVTSSSGYDVSAEEARLREFANADPAEIVNR
                     ALQSQADTVNGGISSDMRSNILGDLSSSRQYATAKAYKSPPRKYLLKRRTNFQCVLYT
                     AVKTDHSGFVGCRLTKPIFSADGSVILAEAGAELNGEQKIEVKQGQSTVFTSWSELET
                     TAGTEGGIRADLNGLGTGPMGSSGTDAYIDNHYGQRFGGAVMLSFIQDAIATAANAAQ
                     KNKSTYSFDNSEDNAESMAEKALENSINIPPTGYVLPGTVINVIVAQDIDFSSVYTVK
                     Y"
     gene            complement(11516..12328)
                     /locus_tag="ACHOWS_004041"
     CDS             complement(11516..12328)
                     /locus_tag="ACHOWS_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFQ0049541.1"
                     /translation="MRKIIKYIVPVCLIFSVTGFVHAEIAGKGSVSDKRIQTALYSPD
                     NVFRIYAMKDRTTMIQLEQGETINTDAGAMNLGKPGTEGAPEWLLGANKAGSLIMIKP
                     SRYAEDPRTNLIVSTNRRTYLFELHLTQSPALATYLFRFNYPKPPEVGKNPFVGRNIN
                     ENPCDGIINKKYQKRGDMIISPYQVWDNGTFTCFRFPTNAPRPVIYEVLPDGTETMVN
                     ARPVNDILVVHGVSREFRIRLNRLVLAVRSKVENTGWYNYKGTTTGEVREVK"
     gene            complement(12312..13175)
                     /locus_tag="ACHOWS_004042"
     CDS             complement(12312..13175)
                     /locus_tag="ACHOWS_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFQ0049542.1"
                     /translation="MLFKSNKIKKETEQERLLHYGDGKLSGKSDEIGREIRAYTMAAR
                     WFEKRVAEDYRKKARNSRILAGLFGVIAIVAVIAVANLAPLKTVEPFVIRVDRNSGYM
                     DVVRPGWSKEDTKDISDDKHFISLYILSRETYNWASQKSNFAIVRQLSYSDVFSEYRN
                     FQLSGKGYVSTLNQSRQISVDIDSIVPLPVSTDKKLGERDDIKTYQVRFRQSLLDAEG
                     RPVPETGQILKLDENGKPIVKPKVVFWTAIISFDYLNTADTEGEAWLNPKGFGVLAYT
                     KTEEIRNEENN"
     gene            complement(13392..>13587)
                     /locus_tag="ACHOWS_004043"
     CDS             complement(13392..>13587)
                     /locus_tag="ACHOWS_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0049543.1"
                     /translation="FTLLATEIPNYAASIMGGAPSGGVGSMSRLLGKATGFGAASHMS
                     KALGSKLVDRFHGRGKGRIS"
BASE COUNT         3878 a         3254 c         2240 g         4215 t
ORIGIN      
        1 tgcttttgtt ttccctaaag tcgcagactt agaaaaagtt tttacccgct tcaccagacg
       61 aaacgtatca ccttctttat tcagataata ttgcctgtca ccttttctca ttttcatatc
      121 tatatgattc tctctaagag aagcctcaag cggagccgaa cgactcgtac actgtacgca
      181 tggtttccct attgcaacag atttaacata cttttccatc ttcaaactcc tgccgctgtg
      241 cggctttggt gacagagaac tatcccttct cgatgcaacg gtaggtcata atcgacctgt
      301 ttgcaagcta ttttttcttt ttaaatgtcg gtacaacgct tccatcacgg ttaagattca
      361 gcacggcatc aaacttagca ccatcttttt tcttaagacc tttaatttcc cgcgtatgac
      421 ctttcatcaa caaatctgta gcctgtgcta aggtcaattc tttaccaaac tgagtacccc
      481 acaatcggaa tccacatcgt tcatttgaac agttataggc ttttggtgat atcttcatcc
      541 cggaaccaca taccgggcat ctaaccttcc tctcaaactc gctacgcgaa aaatcaggca
      601 cgccgcctct gtctggaaca gcattccgac aatcaggata cttactgcat ccccaaaaaa
      661 aacctttgtc ggttctccga cgaacataac ggccaccaca cgtacactga tactctgcta
      721 cggccaggcc agaaatatcc acatcagaca cctgttgcaa aataaactgt tcaagttcat
      781 caagaaaatc gtcgatactg atttcccctg tctcaatcat agattgctgc tcatgccata
      841 gagccgtcat atctggtcgc gtcatgacct ccggtaaagc atgaataaac gataagccta
      901 actctgtagg aaccactttt ttattcttga cagcaataaa accgcgaacc tgaagtacgt
      961 taagaatatg cccccttgtt gccggtgttc caattccccc tcgttcctcc tctttacctt
     1021 cgtctctgtc cagtaaaagt tgtctaatcc tgggatcttc gacataacgg gccacacgcc
     1081 gtagatcctc aagcaatgat gcttctgtat ataatggtgg cggtgtggat gtctcttttc
     1141 gagaattcac actactgcaa accaattgtt gaccttttgt aaggctggtt atggcatcaa
     1201 ataacttaca gtccggcaag gtttcttcgt ctctgtctgc tagtaatata ctccagccag
     1261 gggaaacagg ccgtatcgct cgttgccgga acagaaatcc attgatatta aattcaacta
     1321 ccgcagaatc aaaagtttta tcaggcataa actgcacaag atactgtgcc acaattgcct
     1381 tataaaccct catttcatct tcgttcattt gatccagccc ggtaggcgtt ggtgtcggaa
     1441 taattgctgt atgagcactt attttcttat cattaaatgc cctgcttttt cgcccactat
     1501 ctatatgaga aaaaattttt cccagttcag gaaaaaaaga agaaagcatt gctaatgtcg
     1561 ccggagactg agaaaactgt tcttctgaga gataacggca atccgaacgg ttataagtga
     1621 tagccctgta ttcctccctt aatttttgcg ttatctccaa cactttttca gcagtgaatc
     1681 catactgacg attcattttc acctgaagat caagaagcga aaatggtaat ggtggtctcc
     1741 tgcttttaat ttcactctct gcaagagaaa cactcgcttg ttgtccctta caactacgaa
     1801 ttacttcatc acaatattgc tcgctgataa ttctaccttt atcgtccaca ggtgcatctt
     1861 ccggtggaac aagcatcggt gaaattattg tatcagtatc aataaattcg gcctgaacac
     1921 accaataatc atatttctta taatccctgt tttcttcata ccgccgaacg accagtccaa
     1981 gaactggtgt ctgaacccgt cctactgaaa gtatttcatt atgccctttt tctctggctg
     2041 caatagtgta tgctctggtc atatttaaac caaaaagata atcacctgca ctacgggcaa
     2101 gagctttctg ataaagcccg taaaacgctc ggttatcctg taaattacgc atcgcctgac
     2161 gcactgcatc tttattaaga tcattaatca gaacccgttt aacgggagct ttatttccac
     2221 aataagaaag tatctcttca ataagtaact gcccttcctc atctggatcc cctgcatgaa
     2281 taatcatatc tgcacgagta attaatccag acaaaacatt aaattgtttc tctgtttttg
     2341 ggataacgat atattgcaac ggtcgtaatt gcagtggtaa tgtattttta gaccagcttt
     2401 tatattcagg agttttcaat tcaggtggta ttagctttaa caaatgacca atagcccaag
     2461 aaaccgtatc attgttgcaa actatatacc catcaaaata ctgtgggttt cccagaacct
     2521 gagcaataag cttcccaagc tctggttttt cagctatcca gagtcgcata agaatatctc
     2581 cgcttgaaaa gtacgactgg atgtatgatt taataaatca gcttccagac atttttagga
     2641 attttatcaa ataattgtcc agttacgatt tcagccaacc ggtgatctgc ggctgtcaaa
     2701 aactctacaa aatcaatatc attcattgta tatgccttac gctgaataat tttatcaaga
     2761 gtagaaagct ttgagcactt cctgagctga aacaaatact cctgcttact actttttggg
     2821 tacttcctgt atttgttctc catttaagtt attcctcaaa cggactcaat atcttcaaga
     2881 ctgtcctgtt tttgatttag tattattacc aagcatttgc atagttccct tctgatcaac
     2941 aacaatctct gttgtataat gctcctcccc atctttatct gtccattttc tgtaacgtaa
     3001 tgttccctca atatagacct gcattccttt acataaaaaa tctctggata tctctgccag
     3061 tttgttataa ataacaactc gatgccattc tgtattctct tttatttcac cagactgttt
     3121 atctctccac gtttcgcttg ttgctatact taaattagca acggtaactc cattctgcat
     3181 acaccgaatc tcaggatctt gtccaagatt acctattaat gtaactttat ttataccttt
     3241 agcagccatt taatactcca ctaatcctca agataactta aataatctgg ttgtgccccg
     3301 ttctgtctgt atatatcttc cctttcatca atatcctcct ccgcttcctg tatacttaga
     3361 tcaagagatc cttttaaaaa tgcacgagca tttttaatta tttctgttga taactctttt
     3421 ttagttccgc gaatcattcc aattgatata tttattattt ctttaatttc agcaggtgta
     3481 gttctgtcaa gtaccgcgcc aagctcttta atattgatgt ttataccttc tggtaaatac
     3541 atctttgcac aactagcagc atctttagtc ctgaaagcac caatagaaat aactctgtca
     3601 attcttcccg gacgtttcct gatacgagga tcaattctcc tgggatagtt tgttgagaat
     3661 attacatatg tcccggcagg atttctcggc gtatcagccc catccagaaa acttagcgta
     3721 ccagcatcag ctctataaag ctcatcagcc tcttcagcta taataataag aggtattttc
     3781 ttgttcgctg cggcataaca aacttctcta aaatagttat cagcatatac aaatacatac
     3841 ttatcctgat actttgcccc aagagctttg gctattgtcg ttttacctgt tcctggtggg
     3901 cctgtaaaca gaatcttcct cattccagac tgaccataac gcgtatagta atcaatatca
     3961 gaaaaaaact gattaaaatc gttctccagt tcagaaaatt taggatgacc tgaaaaacgt
     4021 aaggcatttt caatctcttc ttttctagat ttaccgtaat aataaatccc ctgataatca
     4081 cctattttcc agataccttt tcttggtaac gaacgattta gtttaacgcg ccccgcttca
     4141 caaagattat ggagcataaa caaaccttta gttgttccta tagcatattc cattctaata
     4201 ctggaattat gaatatatct gacaatatgc acgacttcat ttttccccaa atgaattctg
     4261 aatgctcctg acccgtcttt aaacatgagt tttttagtcc ttcccacttt aaccattact
     4321 ggattacttc ctgtatgaac aactccagaa gaagctacag gttcgatatc aagatttgaa
     4381 tatgtatttt tatttttcct gtttataaat atttctgcta ctttaggata ataatcataa
     4441 ttttttacaa tagtgccaaa acattcatca ccataatatt ctttatgaag ccgcaaagct
     4501 tgctctacca aagttaattt ccccatatgt tacctttaat aaatcagttg tttttccgcc
     4561 attcccacgg tggaaccgga tttgtatcag cccataaacc acgcttttgt tcctgcgctt
     4621 cccgttgcaa agccggtaat gatttatctg tgttgtaacg ttcatatacc catgcagcgc
     4681 cagactgaac cataaagcgg ctggcatccg tgccgttcgt cgtgaacaca cgaccgataa
     4741 tgcgtccgta gcggtctgtc tgcgtgtacg tcacagtaac aggctgtgct gcaagcagag
     4801 ctttaagctg atttgctgac cagcgcccga aagcctgttt tttctccggt gcatcgatgt
     4861 ttgccaggcg aatcctgacg ggctttttgt cctgtaaaac ttcgatggta tcgccatcga
     4921 gaacacgaat aactttcccc tggatttcct ggtttaatgt agaaagtatt cctgctaaag
     4981 caccaggtat tccacctgca tttgcagcct gaaaggcagt aagcagaaaa ataaatattg
     5041 ttaatgtaat tttcacaaaa tactaacccc ctccggttgt tcaactccgc aaatcccaga
     5101 ttaaaatcag gaatctgcgg agtttatgat ttgcttatcc ttcacattga attaatctgt
     5161 ttgcatagcg aatggcacat tcttctgata ttgcctgttt ccagttatct tcacctacag
     5221 gttgtctgaa cctgtgttca cagccattaa aagcaaaaat cccctcgcga ttaagtatgt
     5281 tggttgcaat atcccactct ttaatcctta aaaatgccgt tactgcaaaa ccagaatatg
     5341 atgcaatttt cgaaatgatg catctgtcag cttttattaa cgcggcaaaa ggaactatct
     5401 tttgacgtcc acacgaaatc agagggagtg tgtgaatatc ataataagct attgaatggc
     5461 aatccatgtg agtatcatca atagctgcca gccagaatcc tgtctggtcc tttatttttc
     5521 tgctaatatc agaatctgaa acaccaggat ttaataaata tccaccataa cagacataga
     5581 atgggtgagc agattcatca atatcaggaa gctcttttac cggatattct ttccagtcct
     5641 tcagaacacc gaactcaaga gcatccagca aaaactttcg ttcaaatcca tcaaacaatg
     5701 catattgcat atactacccc tcacagaatg cctgatttat ataactaatg cgctatcaat
     5761 agatggctgt ctcttaaata taatgagatt cgataatacc agaataacaa cgactaccgt
     5821 catcattcat tttatttcat tgatttcttc aattgaaata tgctttggta tagtcctgaa
     5881 acaagtctct atgagcctga gcggtgcttc tttaccctca ctcgcgcaac ccaacacaat
     5941 cgtagttgta gcatcttttt gagtaagacc ttcctgaata accttgttaa tgtcagtgtc
     6001 agatgacaga gttacaaatc tgatttcgct catatgatta cctcttcaaa aaattagacg
     6061 tcggatatct ttttcatccc aataagtcag tattttcccg gatagcagac ggttaagcag
     6121 ttcctgtttc acatctggtg cgccctctgt cagacgcata agtcgaagca gttcctgacg
     6181 gttcaggacc atatggcggg tgtaataata aataataaaa ctgcatacca tacaaaggat
     6241 agtaaaggaa aataacaggt gtttcagatg tgattccggc aatataacag aaacacagaa
     6301 cgtggcaaca acacaaagaa gacatataat taaattgagt atacccagta ccgtatataa
     6361 agaagcctgt tttctgaata tccgctcatg ttcatccaga atataaccta ttttttcctc
     6421 atctgattta tccatatatt tcattcccat agttgatgtg gaaatagtta ttcctctccg
     6481 caaatctagg ataaacatat gtcatctgcg gagaatagtt caaacgcctc tagtttagtt
     6541 ctaaagcctt tgattatttt attcaaaaaa gttttcttcc aaacttactc ataattttac
     6601 tgacgtgagc aggatctgcc ccagggcaat tcattaaaaa tgcttttctc gcatcaaaag
     6661 ttttactcca ccgaatacgc cctttcttct tttttataat attaaagaat ttttttgttg
     6721 gtcccttaca ttcagatgaa ttttccccat aaacttttcc agccatacac ataaagacag
     6781 tacagggatc ccccgaatct atacttacct cttcagcata gcttttactg gataacagga
     6841 aaagaataat caccgctatg acagaatatt tcatattaac ccccttttta ttaaaaattc
     6901 agtaatcatt atcttcataa gattcccttt ctgattccac ttgtgggatg tttaatcgtt
     6961 ttttctgctc ctcatttaaa taatcacctt ctggtgaaga tatcttaact gcatccgtaa
     7021 tatactgact tattggtaca acaagtcctt taagtttcga tatattcccg gctggtatat
     7081 caggcacggg ataacgaaca ggatccactc ttgctgttaa ttcaggttcc ttgtaccaca
     7141 ttattttttt cacccggcct ttcaaggcgg gattccattt tgttggctgc ataaacacca
     7201 gttcttcagg atatggaagt tcattaacct cctgtggcaa cattaaaggc ctagcatgca
     7261 gtgtaacatt acgtcctcga gaacgacgct gtccttccga tatattcctg ctactggacg
     7321 caactcgttc tgtgtacgtt cccagtcgtt tactgtactc ctccgctgta ctgatatctc
     7381 cgggggcaaa tacaacctca atatggaatg gatccattac agctttcccc cctgcgggtc
     7441 cataacagtc atcagagcaa acctgacttt tattctgaaa aacaattata tagcgtaatc
     7501 ctgcccccct gcttagtgcc ggggctgttt ccataacctt gatacggccc atcacagcaa
     7561 attcatcttc cagaaaaagc acctgatatt tcaatctgag agaaccgtcc tccagatttc
     7621 ccccttgctc cggtagtatt ttactgtttt cccttattgc ctgagaaaaa aacaaattca
     7681 ttagtggacc aaatttctgt aaagctccct cggtaacaca aaaataaatg ctggttggtt
     7741 cttcccttaa cttgctgaag tcaatatttt cttcctcgtt atcactctcc gtcagcgcca
     7801 gcgccaccag tttttcgccg tacaagctta gacgtcccgt gacgaatgac atcaacgttg
     7861 aaaattccct gcctttcgac tcggaaataa tcacccccaa ctctcggacg gtttcttctg
     7921 acagtgcatc tggcgttcct acacgagcct gaataacacg ctgcgcccat tctccaatac
     7981 gagttcccaa cgatgcaatt tcgtaacatt gcggtaaact caatggcaac gatggtgttt
     8041 cgatcaggta taaaactatc gcggtaaaca catctgcggc tttagaaaac caactggcat
     8101 tcttcatgtt atcaggtaca tacagagatg aggctagtgt tcgaacatct cccaagcgat
     8161 acagcggatc ctgatttatt acctttaacg gattccagtg atgggtttcc agtcgctctg
     8221 gcgaaaaacg cacgactttc tgaccacatg cttttctgaa acccgctgtc aaatcccagt
     8281 tttcaaattt acagtcattc actacaacag aatgcggata caatagtaga ttagggatca
     8341 caatccccac ccctttacca cttcccggtg gagcggccaa catcagatag gtctgtccat
     8401 aggatacaag taaattatta ctgtacggat ctttaccaaa aataattggc ggataatgag
     8461 ttactctttt ttggattaac tttccatttt ccagcaccca tccgtctggt attttttgtt
     8521 ttcccattaa tttctgtcct ccttctctgg cccttcatac cagaaacgac gcaattcccg
     8581 ttttccggca aacctagcat taccatgtag gtcctttctg ttgccgtccg ctccatgagt
     8641 catggccaaa agagaaacac ctgccgggaa aaaggtaatt gcaactgtca cacaccacgc
     8701 ccagggaagt aacattctgg cccgatcatt cagtgctggc agtcctccct gacttatcag
     8761 ggtcatcaaa gatggagagg agtcaacacc tgataattca agccacatcc atcctccagc
     8821 gtaaagccca ataacaaaaa acaaacaaca aagagctatt atcacgataa atagtttgag
     8881 tttttccata cagcactacc tcacgatgca aaagaaagag cactttccat gctggcacca
     8941 ttcagtaatg ccagttgcac ctctggctca tacgtaatat ccaagatttc tgttttctcc
     9001 atatacatca caacatcgaa agacgttctg acacgtcgat cgatataaga gatatccagc
     9061 ccggcaccaa ccgcagactg ctttattagt ccagagatac gagcataacc atgtaaactg
     9121 ctattagcat gcgctgtcgt aaccgttcct ggatgcccgg tattcagcac ttccataaaa
     9181 tcccaagttt catcgcctgt cagttctgcc agaagaatat gatccccctt catgcgcata
     9241 caacacgcta ccaggtcttt tggtgtcaca taatctccat acaataaatg tacgtgatta
     9301 agatgataag gcagcttcat ttcattcaat tcttcaatag tcagtaaccg acgatttgac
     9361 gggtaaacat caacaattgc tttagcaaaa gttgtttttc ccgaaccggt tccaccaaaa
     9421 ataataatat ttagtttatg tctcacagcc agacggaaaa attcaacata ttcacgagca
     9481 gcattaagac gttttaattc cttttgccag tccagtaact ctttacttct tcctcttaca
     9541 cgattggcat tatcgaaacg tccactggag tcataattct cgagagagaa acgttgaaga
     9601 gacggtttac gaaacgtcat cgacacagta gatttctcac aggcgggagg aataacaatt
     9661 tgtcctcgtt cgcctcctgg tagttccact gtttttatcg gtgacttaac acctaatata
     9721 cggttattat aaacagccag tgtttcagcc aggtgttcac acatattcag cgttaaccac
     9781 ggtgcatctt ccctttgcca gccatctttt gatgtctcgg tccagatctc aaaaggtctg
     9841 ttaatcgcta cctccgtcac tccctgacgg gacagacgtt ctgtaacacc agactgatcc
     9901 agccacccca aaacaagttc tgacttatcg aggtcaaatt cggtattcat taatagacgc
     9961 cctttttatt tacactttta tgagttactt ttctatctca ttaatgaaat gataaatgca
    10021 gcaacgatca ttatgattga ccaccgtaaa tttattggga tacgataaaa ataaatacac
    10081 acccatttaa aaaatcccat ccaacctcct tcaagcaatt actttaatat atagccaccc
    10141 gttaaggcgg ctatatatca ttaatattta actgtataga ctgacgaaaa atcaatatcc
    10201 tgagcaacta ttacattaat aactgttcca ggtaatacat atcctgtagg tggtatattt
    10261 atagaatttt ccagtgcttt ttcagccata ctttccgcat tatcctcact gttatcaaaa
    10321 ctatacgttg atttattttt ttgtgccgcg tttgcagctg ttgctatcgc gtcctgaata
    10381 aacgatagca tcacggctcc accaaacctc tgcccataat gattatcaat gtatgcatcc
    10441 gtgccactgc ttcccatcgg ccccgtccct aatccattta aatctgctcg tataccccct
    10501 tctgtcccgg cagtagtttc aagctcactc catgaagtga acaccgtaga ttgcccttgt
    10561 tttacttcaa ttttttgttc cccattcaat tcagcacctg cttcagccaa aataacacta
    10621 ccgtccgctg aaaaaatggg ttttgttaac cgacaaccaa cgaatccaga gtgatccgtt
    10681 ttcaccgccg tataaagtac gcactgaaaa tttgttctcc ttttcaggag atacttacgt
    10741 ggaggcgatt tataagcttt agctgttgca tactggcggg atgatgagag atcccccagg
    10801 atgttagatc tcatatccga agatattccc ccattcacag tatctgcctg actctgtaaa
    10861 gcacggttaa ctatctctgc cggatctgca ttggcaaact ctcgtagacg agcctcttct
    10921 gccgaaacat cataaccaga agatgatgtt acaccgtcac tactaccacc aactccttgg
    10981 ctaatgccgg aagaatcaaa tcgtgcaaca ggcgaaaaca acatttctgg tactctgata
    11041 tccgtaaccg gtgtaaccgg gttgaccgta gactggcccc ctgaagtttg agtatttttt
    11101 ttgtctgaaa caggtccgtt actctcctgc tttcctgacg actggacggt tctactgtca
    11161 gattccgttt tgtctctgtc ttgtggtaaa tgagcctcca taaacgctcc cattggatca
    11221 tcaattaccc ctatagagcg agttacgacc tccgctttaa tccctgtttc gtctatttct
    11281 ggttctggat ctgcacgatt attaataact attgcggcaa atataattgt aaacaaaaga
    11341 acaataacaa taccgccaat aattacacca actattttcc agttaacagg cttcttacga
    11401 ccagctggtt ttaaagaaaa tgcgcccaca ccttgacctt gaggcttatt ttcatcagca
    11461 gcgctagttt cctttttcgt ttcattctct ggtggtgtca ctacattttc agtcattatt
    11521 tgacctccct gacttcacct gtagttgttc ccttataatt gtaccatcct gtattttcta
    11581 ctttagaacg tacagcaaga accagtctgt taagacgaat tctgaactca cgactaacac
    11641 catgcacaac caaaatatcg tttacaggac gcgcattaac catagtttct gtaccatcag
    11701 gtagaacttc ataaataact ggacgtggcg catttgtcgg aaaacgaaaa caggtaaatg
    11761 taccattatc ccatacctga taaggcgaaa taatcatatc accacgtttt tggtattttt
    11821 tattaataat accatcacac gggttttcgt ttatattcct cccaacaaat ggatttttac
    11881 caacttctgg cggttttgga taattgaacc tgaacagata tgttgctaaa gccggtgatt
    11941 gtgtcagatg taattcaaat aaatatgtcc ggcgattcgt acttacaatt aagtttgttc
    12001 tgggatcctc cgcatatcga cttggtttta tcattattaa acttccggcc ttattagcac
    12061 cgagtaacca ttcaggtgcc ccctctgttc ctggtttccc cagattcatt gcacccgcat
    12121 cagtatttat tgtctcccct tgttccagtt gtatcattgt tgttctgtcc ttcatggcgt
    12181 aaattctgaa aacgttatca ggactataca atgccgtctg gatccttttg tcgctgactg
    12241 aaccttttcc ggctatttcc gcatgaacaa aaccagtaac tgaaaatata agacatacag
    12301 gaactatata tttaattatt ttcctcattc ctgatctcct ctgtttttgt atatgcaagc
    12361 acaccaaagc ccttaggatt taaccaggct tcaccttccg tatcagccgt atttaaataa
    12421 tcaaaagata ttattgctgt ccagaaaacc actttgggct taactattgg tttcccgttt
    12481 tcatccaact tcagtatctg tcctgtctct ggaacaggcc gcccttctgc atcaagcagt
    12541 gattgtctga aacgaacctg ataagttttg atgtcatccc tctcacctaa ttttttatca
    12601 gtactgacag gtaaaggaac tattgaatca atatcaacag aaatctgacg agactgattt
    12661 aatgttgaga cgtacccttt acctgacaac tgaaaattac gatattcaga aaacacatcg
    12721 gaataagaca actgccttac tattgcaaaa ttagattttt gactagccca gttatacgtt
    12781 tctctagata aaatatataa tgatataaaa tgcttatcat cagaaatatc ttttgtatct
    12841 tccttactcc atccgggacg aacaacatcc atatacccac tattgcgatc tactcggata
    12901 acaaaaggct ctactgtttt aagtggagct aaatttgcaa ctgcaattac agcaacaata
    12961 gctataacac caaacaatcc agccaaaata cgactatttc tagctttctt tctgtaatct
    13021 tcagccaccc ttttttcaaa ccaacgggca gccattgtgt aagcccgtat ttccctccca
    13081 atttcatcac tcttacctga taatttgccg tcaccataat gcaagagacg ttcctgttca
    13141 gtttcctttt ttatcttatt acttttaaat aacatttttt tctcccggta acagcacaga
    13201 ctctggattt acatcaatcc attcaccaga tggttggggc aactctggcg gtgaactaca
    13261 tgatatgcat cccagcacaa cacaaagcgc cgcacatata gcgaatatat ttttcatgat
    13321 aagccttttt ataaatgaag tttttgtaaa gaagacgaga aaatggatta taagcagaga
    13381 tagaaaaatt actaggaaat acgacctttc cctctaccat ggaaacgatc aactaattta
    13441 cttcctaatg ccttactcat gtgacttgca gcaccaaaac cagttgcttt tcctaaaagc
    13501 ctggacatac tccctacccc accagatgga gcgcctccca taatagatgc agcataattt
    13561 ggaatttctg tagccagtaa agtaaat
//
LOCUS       DAZKJR010000133        13503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKJR010000133 DAZKJR010000000
VERSION     DAZKJR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.134"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(148..224)
                     /locus_tag="ACHOWS_004044"
     tRNA            complement(148..224)
                     /locus_tag="ACHOWS_004044"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Met,seq:cat)
     gene            433..1530
                     /gene="mltA"
                     /locus_tag="ACHOWS_004045"
     CDS             433..1530
                     /gene="mltA"
                     /locus_tag="ACHOWS_004045"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFQ0049544.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1607..2413
                     /gene="tcdA"
                     /locus_tag="ACHOWS_004046"
     CDS             1607..2413
                     /gene="tcdA"
                     /locus_tag="ACHOWS_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFQ0049545.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2464..2907)
                     /gene="csdE"
                     /locus_tag="ACHOWS_004047"
     CDS             complement(2464..2907)
                     /gene="csdE"
                     /locus_tag="ACHOWS_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFQ0049546.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2907..4112)
                     /gene="csdA"
                     /locus_tag="ACHOWS_004048"
     CDS             complement(2907..4112)
                     /gene="csdA"
                     /locus_tag="ACHOWS_004048"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFQ0049547.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATTLKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPHLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTPQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4304..4531
                     /gene="ygdI"
                     /locus_tag="ACHOWS_004049"
     CDS             4304..4531
                     /gene="ygdI"
                     /locus_tag="ACHOWS_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFQ0049548.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4882..5799
                     /gene="gcvA"
                     /locus_tag="ACHOWS_004050"
     CDS             4882..5799
                     /gene="gcvA"
                     /locus_tag="ACHOWS_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFQ0049549.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5818..6213
                     /gene="ygdD"
                     /locus_tag="ACHOWS_004051"
     CDS             5818..6213
                     /gene="ygdD"
                     /locus_tag="ACHOWS_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFQ0049550.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6206..7306
                     /gene="rlmM"
                     /locus_tag="ACHOWS_004052"
     CDS             6206..7306
                     /gene="rlmM"
                     /locus_tag="ACHOWS_004052"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFQ0049551.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7350..8081)
                     /gene="fucR"
                     /locus_tag="ACHOWS_004053"
     CDS             complement(7350..8081)
                     /gene="fucR"
                     /locus_tag="ACHOWS_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFQ0049552.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(8139..8561)
                     /gene="fucU"
                     /locus_tag="ACHOWS_004054"
     CDS             complement(8139..8561)
                     /gene="fucU"
                     /locus_tag="ACHOWS_004054"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFQ0049553.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8563..9981)
                     /gene="fucK"
                     /locus_tag="ACHOWS_004055"
     CDS             complement(8563..9981)
                     /gene="fucK"
                     /locus_tag="ACHOWS_004055"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFQ0049554.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10090..11865)
                     /gene="fucI"
                     /locus_tag="ACHOWS_004056"
     CDS             complement(10090..11865)
                     /gene="fucI"
                     /locus_tag="ACHOWS_004056"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFQ0049555.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQIFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11898..13214)
                     /gene="fucP"
                     /locus_tag="ACHOWS_004057"
     CDS             complement(11898..13214)
                     /gene="fucP"
                     /locus_tag="ACHOWS_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFQ0049556.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3280 a         3333 c         3546 g         3344 t
ORIGIN      
        1 atatataaat aagatggatg gctttgatgg agggatatta acggtttact cattgtctca
       61 ggcttcactg aaacgggtaa ggaagcgtaa aaacgaaaaa accgcaagcc aatagtagct
      121 ttgcggtttg tcagcattct aataaattgg ttgcgggggc cggatttgaa ccgacgacct
      181 tcgggttatg agcccgacga gctaccaggc tgctccaccc cgcgtccgtg gatgcgcact
      241 atactctgct cgcgttttca tgcaaccttt tttttacccc gatcacggat tcggcatgat
      301 tttgaacaaa aacaaatcac taaagcctat tttttgtgca aaatttgcgt cagggagagg
      361 ctgctcattt cattaaacgc ggtggtttgt tatcttcgtt gcgccttatt ttttaacctg
      421 aagaagagaa caatgaaagg acgttgggta aagtaccttc ttatgggcac ggttgtggca
      481 atgcttgccg cctgctcttc caaaccaacc gatcgcggac agcaatataa agacgggaaa
      541 tttacccagc ctttctctct ggtgaaccag ccagatgccg ttggcgcgcc gattaatgcc
      601 ggtgattttg ccgagcaaat taaccatatc cgtaattcgt caccgcgtct gtatggcaac
      661 cagagtaatg tttataacgc ggtgcaagag tggctgcgcg caggcggtga tacccgcaat
      721 atgcgccagt tcggcattga tgcctggcag atggaaggtg tcgacaacta tggtaacgtg
      781 cagtttacag gctattacac gccggtaatt caggcgcgcc atacccgtca gggcgagttc
      841 cagtatccga tttatcgtat gccgccaaaa cgtggtcgtc tgccgtctcg tgcggagatc
      901 tacgcaggag cgctgagtga taaatatatt ctcgcttaca gtaactccct gatggataac
      961 ttcattatgg atgtgcaggg tagcgggtat atcgactttg gtgatggcag tccgcttaac
     1021 tttttcagct atgcagggaa aaacggtcat gcctatcgca gcattggtaa ggtgttgatc
     1081 gaccgtggcg aagtgaaaaa agaagatatg tcgatgcagg cgattcgtca ctggggcgaa
     1141 acacacagtg aagcggaggt tcgcgagctg ctggaacaga acccgtcttt cgtcttcttt
     1201 aaaccgcaat cttttgcacc ggtgaaaggg gcaagtgcgg tgccgctggt tggccgtgct
     1261 tctgttgcct ccgatcgttc aattattccg ccaggtacta ccttgctggc agaagtgccg
     1321 ctgctggata ataacggcaa atttaacggt cagtacgaat tgcgtctgat ggtggcgctg
     1381 gatgtcggtg gcgcaatcaa aggccaacac ttcgatatct atcaagggat cgggccggaa
     1441 gccggacacc gcgcaggttg gtacaaccac tatggacgtg tctgggtgct gaaaaccgcc
     1501 ccgggcgcag gtaacgtctt tagcggctga tgtgatattc tgtatgccgt ttttacggat
     1561 atgacagggt gaggggtaac ctcacccttt ttaattctga ggtgctatgt ctgtggtaat
     1621 tagtgatgca tggcgtcagc gttttggtgg cacagcgcgt ctgtatggtg aaaaagcatt
     1681 gcaactgttt gctgacgcgc atatttgtgt ggttggtatc ggtggtgtcg gttcctgggc
     1741 ggcggaagcg ctagcgcgca cggggattgg cgcaatcacg cttatcgata tggatgatgt
     1801 gtgcgtcacc aataccaatc ggcaaattca cgctctgcgc gataacgtcg ggctggcaaa
     1861 agcggaagta atggcggagc gtattcgcca gattaacccg gagtgccgtg taacagtagt
     1921 ggatgacttc gtgacgccgg ataacgtagc gcaatatatg aatgccggtt attcgtacgt
     1981 gattgatgct attgatagtg tacggcccaa agcggcgctg attgcttatt gtcggcgcaa
     2041 taaaatcccg ctggtcacga ccggtggcgc tggtgggcag attgacccga cgcagattca
     2101 ggttaccgat ctggcgaaaa cgattcagga cccgttggcg gcgaagttgc gcgagcgcct
     2161 gaaaagcgat tttggcgtag tgaaaaacag taaaggtaag ctcggcgtgg attgcgtgtt
     2221 ctctaccgaa gcgctggtgt acccgcagtc agacggcacc gtctgtgcga tgaaagccac
     2281 ggcggaaggg ccaaagcgga tggattgtgc ctcaggattt ggcgcggcga cgatggtgac
     2341 cgccactttt ggctttgttg cggtttctca tgcgctgaag aagatgatgg cgaaagcggc
     2401 gcgtcagggg taaacaatca ggtcggatgc gacgcgaacg tcttatccga cctggttctg
     2461 tgcttaaacc tgcttcgccg cagcgataat cgcctcgctt aacgcattca acccctggct
     2521 gcgtgaggcg ctaagttgcg cacgtaatcc cagctcatca aacaatgcca gcggcgactg
     2581 tgcctgcaat tcggcggcgg ttttcccctc aacggcagtc aacaacaccg ccaacaggcc
     2641 gcgcacaatg cgcccttcgc tgtcgccaaa gaaatgcatc ttgccgtttt cagccactgt
     2701 atatcccagc cagacgcggt tttcgcatcc ggcaatctct ttagcctgcg cttttaactc
     2761 ctctggcaaa gccggaagct gtttccccag catgatcaac tggcgatatt tatcttccca
     2821 ttgcgtcaac ggtgcgaagg tattgcgtaa cgtttctgcg gttacggttg tgccgaacgg
     2881 atgtccggcg aattgcgggt ttgtcattaa tccaccaata attccagcgc gcggtcaacg
     2941 gcattcacca gcgcatccac atcactcttt gtattatatg gcgcaaatga ggcgcgcagt
     3001 gtgccggtta cgcctaattc tgccagtagc ggctgagcgc aatgctgccc ggcccgcagg
     3061 gcaataccgt actccgccag cagcgtcacc atatcgctgt gatgaacgcc agcaaaatca
     3121 aaggccagca ggctggaatc ctggcagcga aatgagcgga agccaggacg ttttgccagc
     3181 gcatcttccg ccagcgttgc taagctacgg ctccagcttt cggcctggtt gatatcgtaa
     3241 tctgccagcc attccagcgc cgcgcttaat cctatgacac cagcgacatt tggcgttcca
     3301 gcttccagtt tccacggcgc agattgaggc gtgaagccgt caaaactcac ttcgtgaacc
     3361 attttgccgc cgcccagcca gggcgacatc gcttccagca gttctgattt gccatacagc
     3421 acgccgatac ctgtcgggcc atacagtttg tgacctgaaa aagcatagaa atcaatatcc
     3481 agttgctgaa catccgcggg gaaatgcacc gccccctgag caccatcaac catcaccacc
     3541 atcccggctg aatgagcaaa ggtaatcgct cgcgccagat ccgggcaacc gccagtgacg
     3601 ttcgacattt gtcccagagc aagaattcga ctacgaggtg tgatcagttc tggcaacaaa
     3661 tcgacatccg gcagtcgctg cgcattaagc ggcaatttca ccactttggc tccagtttgt
     3721 tgggcgacca tcagccaggg gacgaggttg gcgtggtgtt ctgccacgct gacaataatc
     3781 tcatcgcccg gttgcagatg cggacgcgca tagcattgcg ccaccatgtt gatggattca
     3841 gtggtgccgc gcgtccagac gatagttttg tcatctggtg cattcagtaa ttgcgccact
     3901 ttctcccgtg cagcttcata acgcgcggtc aggcgttggg cttcggcaaa ctggctgcga
     3961 tggacgtttc cggcgctcag gctgtaaaac tgtcgggtgg cttcaaccac ggcttcaggt
     4021 ttaagcgtgg tcgcggcgct gtcgagatag acgcccgcat cctgtagcgc gggaaactgg
     4081 gcgcgaaact gcgcgggatt aaaaacgttc atggtactcc tcggcttgat gcaccgatcg
     4141 tgacgcaaaa ttcactttga tacaaggaga gtgctaacca tcagaattat ctggatatcc
     4201 tggcgaagcc gcgtcagtaa catatgctga atagtgttag gcgttaattt tagcctgtta
     4261 atcaatacta ttaagattta ttaatcgctt taaggagaag atgatgaaaa agactgccgc
     4321 aattatttct gcctgtatgc tgacttttgc cctgagcgcc tgttccggtt cgaactatgt
     4381 gatgcacacc aatgacggac gtaccatcgt ctctgacggc aaaccacaga ctgataacga
     4441 taccggtatg atttcgtata aagacgctaa tggcaacaaa cagcagatca accgtaccga
     4501 cgtgaaagag atggtcgaac tggatcagta attcgcgatc gcaaggtaaa aaaaagcacc
     4561 gcaattaggc ggtgctacat taatcactat ggacagacag ggtaaatgta caggaagtga
     4621 aaaaaggtag ctttgctacc atggtctgaa tcgcagacca attgcaaaca caacaacaca
     4681 acatcacaac cgtaagccaa aagttcacca gaacacgcat tccgataaaa cttttcgttc
     4741 cggctcagga agtgccgcca ctataggtat ttgctggtag aagctcaacg gacaatttat
     4801 aatggctcag attaaaaaaa ctaataggtt acatagtgta atctaattgt taaattcatt
     4861 taacatcaaa gtttaaaagc catgtctaaa cgattaccac cgctaaatgc cttacgagtt
     4921 tttgatgccg cagcacgcca tttaagtttc actcgcgcag cagaagagct ttttgtgacc
     4981 caggccgcag taagtcatca aatcaagtct cttgaggatt ttctggggct aaaactgttc
     5041 cgccgccgta atcgttcact cctgctgacc gaggaagggc aaagctattt cctcgatatc
     5101 aaagagatat tttcgcaatt aaccgaagcg acgcgtaaac tccaggcccg tagcgctaag
     5161 ggggcgttga cggtcagttt actccccagt ttcgccattc attggttggt tccgcgactt
     5221 tccagcttta attcagctta tccgggaatt gacgttcgaa tccaggcggt tgatcgtcag
     5281 gaagataagc tggcggatga tgttgatgtg gcgatatttt atggtcgggg caactggccg
     5341 gggctacggg tggaaaaact ttacgccgaa tatttattgc cggtgtgttc gccgctactg
     5401 ctgactggcg aaaaaccctt gaagaccccg gaagatctgg ctaaacatac gttattacat
     5461 gatgcgtcgc gtcgtgactg gcagacatat acccgacagt tggggttaaa tcatatcaac
     5521 gttcagcaag ggccaatttt tagccatagc gccatggtgc tgcaagcggc tatccacggg
     5581 cagggagtgg cgctggcaaa taacgtgatg gcgcaatctg aaattgaggc cggacgtctt
     5641 gtttgcccgt ttaatgatgt tctggtcagt aaaaacgctt tttatctggt ttgtcatgac
     5701 agccaggcag aactgggtaa aatagccgcc tttcgccaat ggatcctggc gaaagccgct
     5761 gctgaacaag aaaaattccg ctttcgttat gaacaataat ttacgtaggg tacgaccatg
     5821 accagccgtt ttatgctgat tttcgccgcc attagcggct tcatttttgt agctctgggc
     5881 gcttttggcg cgcatgtgtt aagtaaaacc atgggggccg ttgagatggg ctggatccag
     5941 accggcctcg aataccaggc gtttcatacg ctggcgatct taggtctggc ggtggcaatg
     6001 cagcgtcgca tcagtatctg gttttactgg agtagcgttt tcctcgcgtt aggcacggtg
     6061 ctgttcagcg gcagccttta ttgcctggcg ctgtcccatc tgcgtttgtg ggcgtttgtc
     6121 acaccggttg gcggcgtgag cttccttgcg ggctgggcgt taatgttagt tggtgctatc
     6181 cgtttaaagc gcaagggcgt aagtcatgaa taaggttgta ttgctgtgcc gtccgggctt
     6241 tgaaaaagag tgcgccgcag aaattaccga taaagccggc cagcgggaaa ttttcggttt
     6301 tgcccgcgtg aaagagaatg cgggttatgt catttatgaa tgttatcaac ctgatgatgg
     6361 cgataagtta atccgtgagc tgccgttcag ttcattaatt tttgcccgcc agtggtttgt
     6421 ggtgggggaa ctcctgcagc atttgccgcc agaagatcgt attaccccca ttgtcggcat
     6481 gttacagggt gtagtagaga agggcggtga actgcgtgtt gaagttgccg ataccaacga
     6541 aagcaaagag ttgctgaaat tctgccgtaa attcaccgtg ccgctacgcg ctgccttgcg
     6601 cgatgcgggt gtgctggcga actatgaaac gccgaagcgt ccggttgtgc atgtattctt
     6661 tattgcacca ggctgctgct ataccggtta ctcatacagc aacaataatt cgccgttcta
     6721 tatgggcatt ccgcgcctga aatttccggc agatgcgccg agtcgttcca cgctcaaact
     6781 ggaagaggca tttcatgtgt ttattcctgc ggatgagtgg gatgaacgcc tggcgaacgg
     6841 gatgtgggcg gtggatttag gcgcttgccc aggcggctgg acctaccaac tggtgaagcg
     6901 caacatgtgg gtttattccg tcgacaacgg cccgatggcg caaagtctga tggataccgg
     6961 acaggtgacg tggctgcggg aagacggttt caaattccgt ccgacacgca gcaatatctc
     7021 ctggatggta tgcgatatgg ttgaaaaacc ggcgaaagtt gcggcattga tggcgcagtg
     7081 gctggttaat ggctggtgcc gtgaaaccat tttcaacctc aaactgccga tgaaaaaacg
     7141 ctacgaagaa gtgtcacaca atctggcgta tattcaggca cagcttgatg aacatggcat
     7201 aaatgctcag attcaggcgc ggcagttgta tcacgatcgc gaagaagtga cggtgcacgt
     7261 ccgccgtatc tgggctgcgg tgggtggtcg tcgcgacgag cgataacagg caaggccggg
     7321 gcgtcgtttc cggcctgcta tcctttccgt caggctgtta ccaaagaagc tgcaacctgg
     7381 cgctcatcag aaataatgtg cgtgacctca tccagatgcc cgatgcgggc ttcccctgaa
     7441 cgattaaatt tacttttatc aatcaataac aacgattgcg cggcacgttt taatagcatc
     7501 gatttgtaat cagcgttgat ggcattggag tcccacagtg cgccgctgct atcgatccct
     7561 tcacaagaaa aaataaacag atcgatttcc agcgatttaa gctgggaaat cagcgaggga
     7621 ttgacgtaac aaccatattt gcgctcaagt gtgccgccgg aactgatcag ttgaatgcgt
     7681 tcgcgtttac cgagttcatg gcaaatcgga tggctattgg tgaagacctg gatgttgatg
     7741 tcaggcaact ggcgtgccag ataccagcag gttgaacttg cgtctaaggc tattaccatc
     7801 ccttcttcaa tccacgcgag cgcctcgcgc gcgatatctg ctttatgcgc ataatggctt
     7861 ttcagccgga tgtgaaaggg atcgccactg tcttgatttt gacggtggat atatttagcg
     7921 cgcccatgat tgcgcagaat tttaccctgc gtctgtaatt cattgagatc gcgacgaatg
     7981 gtttctttac tgacctttag ctgttcagag agagcttccg tggtcaggct ggtatggttc
     8041 agcagcaggt cgactatcgc ttgctggcgt gccgctttca tatttttcac cccatcattc
     8101 gataaccgcc cgcgtcatgc gggcgtaccg gtatgagatt acggtgttac cccttttttt
     8161 aaaagaatat tcccgtactt cgctcgttcg cctgtgataa cgatcgcaaa ggctttttgt
     8221 gcccgttcat aaaacgcaaa acgattgatg cggatgatgt caggacaggg ggcttgtagt
     8281 gaaagcgcat tacgataacg tcgttctact tcaggatcta gagtgtcacc ttctaccgcc
     8341 gccatcatca ccagcggtgg tgcataactg tccagctcaa ataacgggat aatcgcctgg
     8401 agcaagtcgc tcaccaacag gccatcagcg cggatcacct gcggtcccat cgaatgggcg
     8461 ggaaagtgag catcggaaaa aataatttca tctccatgcc ccatctctgc cagcactttc
     8521 aatagttcgg gagaaattaa cggcgaaatt gttttcagca tttcacacct cctctataaa
     8581 ttcaggttca gtttgcgggt agaaataacg gtactgataa tgaatctgcg cgcgggcttc
     8641 ttccggactg ttaaattccc ctacgccata ccaaccaaac agcgcagctc ctgcgacggt
     8701 cgtttcggca tcgtcgagaa cttttaccgg aatatcaagc atattggctt taatctgatt
     8761 ccacaatgtg ttgcgacttc ctccgccgac taacaataat tcagaggctt taaagtgccc
     8821 gattttttcc agcatctgta gattgcgctg taattgcgtg gttaaccctt ccagcgccgc
     8881 gcgatagaaa tgcccccgcg tggtattaag cgttacgcct tgccagccag cgttctggca
     8941 cgacaataaa tcacactgca ttttcactcc atctgcgcca ggcgcgatca ggcgagcttc
     9001 ttcaatcaat atttgccagg gagtttcagc tgtccagaac agttttctca cccattccag
     9061 cacgccggat gccagccatt gcatacctgg gttatacaac cctgcctggc tatccagttc
     9121 gcaagtagaa ccggcgtact gacttaacag cgaagtatca acctgggcgc tgcggaccat
     9181 taaaatttcc catgtaccgg aagagagcac gggttcattt tgttcggcac cagcgccaaa
     9241 aagggcgaac tgggtatcgt gacctgcgga aatcaccggt atgccaacgg gtaagccgag
     9301 cattgctgcg gcgctgttct gtagcgtacc aatctgttca cccgcttcca ccagacgagg
     9361 gaagagtcgg cgtggaatac cggtggcttg taaaatttgc ggactgaaat cgcgttgctg
     9421 gatatccagc atctggctgg ttccggccat cgtaatatca gtagtgaatt cgccggttaa
     9481 acggtggtta atcagtgacg aaataaagag ccaggcgtgc gcgcgttcca gcagttgtgg
     9541 atgattttct ttcaaccaca ccaacttata taacgtattg aaactaaagg ctccgactcc
     9601 agaaatagcc tgcaactgct gtgcggagat caaccgttca atattgtcca ttaccgcggc
     9661 tgttcgcgga catttccagc taatgatcgg atagagcaga ttgccttgct tatctaccag
     9721 agcgccatcc acaccaaagg tggtgacggc gataccgcgg atatggcaat cagtcagttc
     9781 actattgatt tgccgacagc aatcagcaaa gcgttgcaaa atggcgtcta aagaccactg
     9841 gtgccaggtg ttgttttcca tcgcgatatc gctggcatta ggcgttgagg cgcgggcaac
     9901 aatttttccc tgccgattaa ccgcgatggc cctgacattg gtcgcgccac agtcgagtac
     9961 caggataact tcttgtttca taatcgctcc tgcaatatag ccggataaca ttgcttatcc
    10021 ggctaaccac tcttgcttgt tcaggcccgg tagccggagc gaccgggcat cacatcaggg
    10081 agtcatgtat taacgcttgt acaacgggcc gtagttctgg caagcgcggt aatcctggcc
    10141 ttcaatatcc atgccgtgcg cggcccaggc agaaggacga tacactttgg tctcttcaac
    10201 gttgtgcata cataccggga tacgcagcat ggaggcaaga gtgataaagt ctgcgccaac
    10261 gtggccgatg gtcagaactc cgtggttagc gccccagttc gccattaccg agtacacatc
    10321 cgtaaacggc cctttaccgg tgaggcgcgg tgcaaaccag gtggttggcc aggttgagtt
    10381 ggtgcgtttg ttgaggatgt catgcacatc cttcggcaat tccacgctcc agccttccgc
    10441 gatttgcagt accggtccca ggcctttgat gatgttgaca cgagtcatgg tgaacgggac
    10501 gccgccttcg gtaaggaagc gggaagagta accgccgcca cggaagtatt cgtggatcgc
    10561 cgggcaccat tcggtagcgg cgaggcaagc gtcagcctct tgctgagaga tttcccagtg
    10621 cggcttcatc gtcggtttac cttcgctgtc gcgttgttta caggagccgt ccagcgcagc
    10681 agaaccggag ttgatcaaat ggatgatgcc gtgttctgcc agcccgtcca gtttatgccc
    10741 cgttacacgc tcaattgctt ctggtgacca gtaggtacgc acatcggcaa atatctgagc
    10801 ggtgccggtg agctggtggc ccattaacat tgccacgccg ttaagactgt cgttttcggt
    10861 cgccacgaca aacggtttgc gcacgccatt ccagtcaaat gaactgttga ggatcgcttc
    10921 agcggtatcg ccattgggat attgatcggt ccagtgacgt tgcccctgga agcctgcagc
    10981 gatggcgttg tagccaagtg attcttccac gcggccaata tcggccagtt tgctgttgcc
    11041 ttgcatcatg tcgcggatac acatcgccat cagtaaactt tcgcgcagaa ctgcgcggct
    11101 ttgctcggca ttgcgttgat actgtttgtt attttcatct tcgccatagc ggaagttttt
    11161 atcagcccag gccagcgcca tttccagttc ggcttcgtcg taaatcttct gatcgatacg
    11221 gcgacgcagt tcggtcatat ccaccgcctg gactttcatt cccagccagg attcaaagaa
    11281 gttgtgatca acgatggaac cggcgatacc catcgaaacg ccgcccagcg acagatagct
    11341 tttacctttc atgctggcga ctgctaaacc ggcgcgggca aagcgcagca gtttttcttc
    11401 aacatcggca ggaatcgatg tgtcatcggc atcctgaacg tcatgaccgt aaatggagaa
    11461 tgccgggatg cctttctggc tgtgagctgc cagagccgct gccaggtaaa cagcgccggg
    11521 acgttcagtg ccgttaaagc cccaaatggc tttcgggcgg gttggatcca tatcgatggt
    11581 ttcactgcca tagcaccagc aaggggttac cgtaatggtg aggcctacat tctgactgct
    11641 gaatttttct tcgcaagcag cggcttcagc catacccgcg atacaggtat cggaaatgac
    11701 acactcgacg gcagctccac aggcatggcg cagtttctcg gtcagcagtg cagccgtggc
    11761 tttcgccata ttcattgttt gttcttcaag cgactcacga acacccatgc gacgaccgtc
    11821 aataaccggg cggataccaa ttttcggtaa gctgattttt ttcattacag attcctcact
    11881 ttattcggaa aatatgttca gttagttgct gtttgagaac ggaaacgggc aaagataaag
    11941 atgaccgcga agcagagtgc ggggatcagt tcagcagtgg ggatgttgcc cgccgcgtca
    12001 ctgacaaaac ccatgaccgg agtgacaata ccgccgccaa tgatggtcat aacgatgaag
    12061 gacgaaccgt atttggtgtc ctggccgaga ttcttaatgc ccagcgagaa gattgttggg
    12121 tactgaatcg acataaaggc gctgcataaa gtcagggcta ttaagcccac atgaccgcca
    12181 gcgaaggctg agatcaggca cagtgccata gcgattaatg cgtaggctgc caggactttg
    12241 tgtggtgcga agcgactgat gagccaggta ccggtgaaac gaccaataaa gaagcacacc
    12301 atggttccgg ttaaatagtt agcggcaaag cctgcggtca tacctggaat ttcttctaca
    12361 gcgtagcgaa tcaaatagct ccagcaggcc gtttgcgcgc cgacatagca gaattgcgct
    12421 aataccgccc agcgccagtg acgaatacgc gccagacgag aaagcgatgc ggagaacgat
    12481 ccttgtttgg cgtcactgtg attatcactc tgcaatgccg ggaatttcgt cagcatgatc
    12541 agcagggcga ccagtaacac gatagccacg atgatcatat aaggtgtctg taccgataat
    12601 accaggctgt gtttatacgc actcaattgc tctggagaca ttttatcgag aacgtcttgc
    12661 gattgatgtg gcacgttaga caaaataaga ctttgtccaa agacaaccgc gataattgcg
    12721 ccaaacgagt taaatgtttg cgcaagattt aagcggaagt gaccactgct ttccggccct
    12781 aataccgtaa caaaagggtt tgcggcagtt tccagacaac ctaatccagc tgcaataata
    12841 aataggccaa ctaaaaataa ggtgtagttc attatttctg cagcgggcca gaataatgca
    12901 gcgcccaagg catataaaaa taatccggta ataatccctg ctttataact gagttttttc
    12961 atcaatatcc cagcagggat tgggataatg aaataaccaa agtaaaaggc cgattggatc
    13021 aggccagcct ggaaatttgt cagcgtaaaa gcctgctgga attgaggtaa taaaatgtcg
    13081 ttaaggttat tggctaccgc ccaaagaaaa aacagtgagc acagcagcgc gaatggaata
    13141 atgtaacttc tgctttgccc tgcatcttta tctaccgcac ggtaactctg cgtttgtatt
    13201 gatgtgtttc ccatagcatc ctcttaggtt cagaagctta agcacgtaat cggttatgtt
    13261 ttcaaaagtg caaaaccagc gagttcgggc ttcatatcag tgaatatttg cctgtcagaa
    13321 atgaactcat ggattcattt tcaaataaaa aacgctctgt aatatgacgg cggtcacact
    13381 tattagttgg gaggtgactt tatgtgacta ccatcacttt aataatcgaa atttcatgcc
    13441 cgtttcagaa aataaatcat aaaaaacaca aacccgatga ccgtattaat tgtttgtgcc
    13501 gaa
//
LOCUS       DAZKJR010000134        13161 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKJR010000134 DAZKJR010000000
VERSION     DAZKJR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.135"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            176..511
                     /gene="yjdM"
                     /locus_tag="ACHOWS_004058"
     CDS             176..511
                     /gene="yjdM"
                     /locus_tag="ACHOWS_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0049557.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            671..1114
                     /gene="yjdN"
                     /locus_tag="ACHOWS_004059"
     CDS             671..1114
                     /gene="yjdN"
                     /locus_tag="ACHOWS_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFQ0049558.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1247..2035
                     /gene="phnC"
                     /locus_tag="ACHOWS_004060"
     CDS             1247..2035
                     /gene="phnC"
                     /locus_tag="ACHOWS_004060"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0049559.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2060..3076
                     /gene="phnD"
                     /locus_tag="ACHOWS_004061"
     CDS             2060..3076
                     /gene="phnD"
                     /locus_tag="ACHOWS_004061"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0049560.1"
                     /translation="MNAKIIASLTFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLTKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3182..3961
                     /gene="phnE"
                     /locus_tag="ACHOWS_004062"
     CDS             3182..3961
                     /gene="phnE"
                     /locus_tag="ACHOWS_004062"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0049561.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3982..4707
                     /gene="phnF"
                     /locus_tag="ACHOWS_004063"
     CDS             3982..4707
                     /gene="phnF"
                     /locus_tag="ACHOWS_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFQ0049562.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     HFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4708..5160
                     /gene="phnG"
                     /locus_tag="ACHOWS_004064"
     CDS             4708..5160
                     /gene="phnG"
                     /locus_tag="ACHOWS_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFQ0049563.1"
                     /translation="MHADTATRQHWMSVLAHSQPTELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLNADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5157..5741
                     /gene="phnH"
                     /locus_tag="ACHOWS_004065"
     CDS             5157..5741
                     /gene="phnH"
                     /locus_tag="ACHOWS_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFQ0049564.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5741..6805
                     /gene="phnI"
                     /locus_tag="ACHOWS_004066"
     CDS             5741..6805
                     /gene="phnI"
                     /locus_tag="ACHOWS_004066"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFQ0049565.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVQEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6798..7643
                     /gene="phnJ"
                     /locus_tag="ACHOWS_004067"
     CDS             6798..7643
                     /gene="phnJ"
                     /locus_tag="ACHOWS_004067"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFQ0049566.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMNMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7640..8398
                     /gene="phnK"
                     /locus_tag="ACHOWS_004068"
     CDS             7640..8398
                     /gene="phnK"
                     /locus_tag="ACHOWS_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFQ0049567.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8509..9189
                     /gene="phnL"
                     /locus_tag="ACHOWS_004069"
     CDS             8509..9189
                     /gene="phnL"
                     /locus_tag="ACHOWS_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFQ0049568.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            9186..10322
                     /gene="phnM"
                     /locus_tag="ACHOWS_004070"
     CDS             9186..10322
                     /gene="phnM"
                     /locus_tag="ACHOWS_004070"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFQ0049569.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGKIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTEAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10322..10879
                     /gene="phnN"
                     /locus_tag="ACHOWS_004071"
     CDS             10322..10879
                     /gene="phnN"
                     /locus_tag="ACHOWS_004071"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFQ0049570.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10866..11300
                     /gene="phnO"
                     /locus_tag="ACHOWS_004072"
     CDS             10866..11300
                     /gene="phnO"
                     /locus_tag="ACHOWS_004072"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFQ0049571.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYQLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            11302..12060
                     /gene="phnP"
                     /locus_tag="ACHOWS_004073"
     CDS             11302..12060
                     /gene="phnP"
                     /locus_tag="ACHOWS_004073"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFQ0049572.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHSGLLDFSHTVEPFVMFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(12088..12417)
                     /gene="yjdP"
                     /locus_tag="ACHOWS_004074"
     CDS             complement(12088..12417)
                     /gene="yjdP"
                     /locus_tag="ACHOWS_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFQ0049573.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12486..12935)
                     /gene="rpiB"
                     /locus_tag="ACHOWS_004075"
                     /gene_synonym="alsI"
     CDS             complement(12486..12935)
                     /gene="rpiB"
                     /locus_tag="ACHOWS_004075"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFQ0049574.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASHVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         2950 a         3946 c         3659 g         2606 t
ORIGIN      
        1 aaatggaggc aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa acgatcgtcg
       61 tctaaaatca gcagtacccc cgacaaactc agggattttg tgtataattg cggccttttt
      121 cggcaatctg ccgttttttg gcgcttttgc cctgctgact tttgaggaaa tccacatgtc
      181 attaccacac tgcccaaaat gcaactccga atacacctac gaagataacg gcatgtacat
      241 ctgcccggaa tgtgcctacg aatggaacga cgcagaacct gcacaggaaa gcgacgagct
      301 gatcgttaaa gatgctaacg gcaatctgct ggctgacggc gacagcgtta ccatcattaa
      361 agatctgaaa gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag tgaaaaacat
      421 ccgcctggtt gaaggcgacc ataacatcga ttgcaaaatc gacggttttg gtccgatgaa
      481 actgaagtct gagtttgtga aaaagaactg attgtattgt gatcggtagg ccggataagg
      541 cgtttacgcc gcatccggca acggtggcga ctgcctgatg cgacgctgac cgcgtcttat
      601 caggcttccc tctcatctgt ataaatttcg aactacactt aacgggcttc tcttaactga
      661 ggtcaccatc atgccgttaa gtccctacct ctcttttgcc ggtaactgtt ccgacgcgat
      721 tgcctattat caacgtacgt tgggcgcgga actgctctat aaaatcagct tcggcgaaat
      781 gccgaaatca gcgcaggaca gcgccgagaa ctgcccttcc ggaatgcaat ttcccgatac
      841 cgccatcgct catgccaacg tgcgcattgc aggaagcgac atcatgatga gcgatgctat
      901 accgtcagga aaagccagct actccggctt tacgctagtg ctcgattcgc aacaggtcga
      961 agaaggaaaa cgctggtttg ataaccttgc agctaacgga aaaatcgaaa tggcctggca
     1021 ggaaactttc tgggcgcatg gcttcggcaa agtcaccgat aaatttggcg taccgtggat
     1081 gattaatgtc gtcaaacaac aaccaacgca ctaacccgcc gggagggccg ccctcccgca
     1141 ctgtcatcga aatctcgtta actcttcatc cgttagtcac ttttagttaa caaaatcgtc
     1201 acaataatcc gccacgatgg agccactttt ttagggaggc tgcatcatgc aaacgattat
     1261 ccgtgtcgag aagctcgcca aaaccttcaa tcagcatcag gcgctgcacg cggttgatct
     1321 gaacattcat cacggtgaaa tggtggctct gcttgggccg tcgggttccg gcaaatccac
     1381 ccttttacgt cacttaagcg gtttgattac cggcgataaa tctgccggta gccatatcga
     1441 gctgctgggc cgcacagtcc agcgcgaagg ccgcctggcc cgcgatatcc gcaaaagccg
     1501 cgccaatacc ggctacatct tccaacaatt caacctggtg aatcgcctga gcgtactgga
     1561 gaacgtgctg attggcgcgc tcggcagcac gccattctgg cgcacctgtt ttagctactt
     1621 cacccgcgag cagaaacagc gcgcgttaca ggcgctgacc cgcgttggca tggtgcactt
     1681 tgcccatcag cgcgtttcca ccctctccgg cggacagcaa cagcgtgtgg cgattgcccg
     1741 cgcgctgatg cagcaggcga aggtgattct ggcggacgaa cccatcgcct cgctggaccc
     1801 ggaatcggcg cgcatcgtga tggacaccct gcgcgacatt aaccagaacg acggcattac
     1861 cgtggtcgtc acgctgcatc aggtggatta cgccctgcgc tactgcgaac gcatcgtcgc
     1921 cctgcgccag gggcacgtct tctacgacgg cagcagccaa cagtttgata acgaacgttt
     1981 tgaccatctc taccgcagca tcaaccgcgt cgaagaaaac gcgaaagctg cctgacatcc
     2041 ccatcattga ggaaaacgaa tgaacgctaa gataattgcc tcgctgacct tcaccagcat
     2101 gttcagcctc agcaccctgt taagcccggc gcacgccgaa gagcaggaaa aggcgctgaa
     2161 tttcggcatt atttcaacgg aatcacagca aaacctgaaa ccgcaatgga cgccgttctt
     2221 acaggatatg gagaagaagc tgggcgtgaa ggtcaacgcc ttctttgccc cggactacgc
     2281 gggcattatc cagggaatgc gcttcaacaa agtggatatc gcctggtacg gcaatctgtc
     2341 agcgatggaa gcggtggatc gcgccaatgg ccaggtcttc gcccagacgg tcgcggcgga
     2401 tggatcgccg ggttactgga gcgtgttgat cgtcaacaaa gatagtccga tcaacaacct
     2461 gaacgatctg ctgaccaagc gtaaagatct caccttcggc aatggcgatc ctaactccac
     2521 ctcaggcttc ctcgtccccg gctactacgt cttcgccaaa aacaatatct ccgccagcga
     2581 cttcaagcgc accgtcaacg ccgggcatga aaccaacgcg ctggccgttg ccaacaagca
     2641 ggtggatgtt gccaccaaca acaccgaaaa cctcgacaag ctgaaaacct ccgcaccgga
     2701 gaagctcaaa gaactgaagg tgatctggaa atcgccgctg atcccaggcg atccgatcgt
     2761 ctggcgcaaa aatctgtccg aaaccaccaa agacaagatc tacgacttct ttatgaacta
     2821 cggcaaaacg ccggaagaga aagcggtgct ggaacgcctg ggctgggccc cgttccgcgc
     2881 ctccagcgac ctgcaactgg tgccgattcg ccagctcgcg ctgtttaaag agatgcaggg
     2941 cgtgaaaggc aataaagggc tgaatgagca ggacaagctg gcaaaaacca gcgccattca
     3001 ggcgcaactg gatgacctgg accgcctgaa caacgcgtta agcgcgatga gttcggtgag
     3061 taaagcggtg cagtaaatcg taggtcggat aagacgcccc ggcgtcgcat ccgacaatgt
     3121 gcagacgttg atgccggatg cggtgcaagc accttatccg gcctacagac cggagccaaa
     3181 catgcaaacc atcaccatcg ccccacccaa gcgcagctgg ttctcgcttc tgagctgggc
     3241 cattgttctc gccgtgttgg tcgtctcgtg gcagggcgcg gaaatggccc cgcttacgct
     3301 gatcaaagac ggcggcaaca tggcaacctt cgccgccgac ttcttcccgc ccgatttcag
     3361 ccagtggcag gattacctca ccgaaatggc cgtcacgctg caaatcgccg tctggggcac
     3421 cgcgctggcg gtggttctct ccatcccctt cggtctgatg agcgccgaaa acctggtgcc
     3481 gtggtgggtt taccagcccg tacgccgcct gatggacgcc tgccgcgcca ttaacgaaat
     3541 ggtcttcgcc atgctgttcg tggtcgccgt cggccttggc ccgttcgctg gcgtgctggc
     3601 gctgtttatc cacaccaccg gcgtgctctc caagctgctt tccgaagcgg tagaagcgat
     3661 cgaacccggc ccggtggagg gcattcgcgc caccggtgcc aacaagctgg aagagatcct
     3721 ctacggcgtg ctgccacagg tgatgccgct gctgatctcc tactccctct atcgcttcga
     3781 atccaacgtc cgctcggcga ccgtcgtcgg catggtcggt gcaggcggga tcggcgtcac
     3841 cctgtgggaa gcgattcgcg gtttccagtt ccagcaaacc tgcgccctga tggtgctcat
     3901 catcgtcacg gtcagcctgc tggatttcct ctctcaacgg ttgcgtaagc actttatctg
     3961 ataagcgagg cattgatagc tatgcacttg tctacacatc cgaccagcta cccaacacgc
     4021 tatcaggaga tagccgcaaa acttgagcag gagcttcgtc aacactaccg ctgcggcgac
     4081 tatcttcccg ccgagcagca actggcagcg cgctttgagg tgaaccgcca caccctgcgc
     4141 cgcgccatcg accaactggt ggaaaaaggt tgggtacagc gccgtcaggg cgtcggcgtg
     4201 ctggtgctga tgcgcccgtt cgattacccg ctcaacgccc aggcgcgttt tagccagaac
     4261 ctgctggacc aaggcagcca tcccaccagc gaaaaattgc tatcggtgct gcgccccgcc
     4321 tccggtcacg tcgctgacgc gctggggata accgaggggg agaacgtcat ccacctgcgc
     4381 accctgcgcc gggtcaacgg cgtcgcgctc tgtttaatcg accactactt tgcggacctc
     4441 accctctggc cgacgctgca acatttcgac agcggttcgc tgcacgattt tctgcgcgag
     4501 caaacgggga ttacgctacg ccgcagtcag acgcggatca gcgcccgccg cgcccaggcc
     4561 aaagagtgcc agcgtcttga aatcccgaat ctatcgccgc tgctgtgcgt gcgcaccctt
     4621 aaccaccgtg acggtgaaag cagcccggcg gagtactccg tcagcctgac gcgcgccgac
     4681 atgattgaat tcactatgga gcactgaatg cacgcagata ccgcgacccg ccagcactgg
     4741 atgtccgtac tggcgcacag ccaaccgact gagctggcag cccgcctgaa cgcgctgaac
     4801 atcaccgccg actatgaagt aatccgcgcc gctgaaaccg gcctggtgca gattcaggcg
     4861 cggatgggtg gtaccggcga acgctttttt gctggcgacg ccacgctcac ccgcgccgcc
     4921 gtacgcctga ctgacggcac gctcggctac agctgggtgc tggggcgcga caaacagcac
     4981 gccgaacgct gcgcgctgat tgacgcgctg atgcagcaaa cccgccattt tcaaaactta
     5041 tcagaaactc ttattgcccc gctgaacgct gaccgtatgg cacgcattgc cgcacgccag
     5101 gccgaagtaa acgccagccg ggtcgacttc tttacgatgg ttcgcggaga taacgcatga
     5161 ccctggaaac cgcttttatg cttcccgtgc aggatgccca gcacagtttt cgtcgcctgt
     5221 taaaagccat gagcgagccg ggcgtgattg tcgccctgca tcagctcaaa cgcggctggc
     5281 agccgctgaa tatcgccacc accagcgtgc tgctgacgct ggccgataac gacacgccgg
     5341 tgtggctttc tgcgccatta agtaacgata tcgtcagcca gagcctgcgt tttcatacca
     5401 acgcgccgct ggtcaatcag ccggaactgg cgaccttcgc ggtaacggat gaggcgattt
     5461 ccagcgaaca gcttaacgcc ctttccagcg gcaccgctgt tgcgccagaa gcgggcgcga
     5521 cgctgatttt acaggtcgtc agcctgagcg gcgggcgcat gctgcgcctt actggcgcgg
     5581 gtattgccga agaacgaatg atcgccccgc agctgccgga gtgcattctg cacgaactta
     5641 ccgagcgccc gcacccattc ccgctcggca tcgacctgat cctgacctgc ggcgagcgcc
     5701 tgctggctat cccgcgaacc actcatgtgg aggtatgctg atgtacgttg ccgtgaaagg
     5761 gggcgagaag gcgatcgacg ccgcccacgc cctgcaagag agccgacgcc ggggcaatac
     5821 cgatttgcct gaactgagcg tcgcccagat tgaacagcag cttaatcttg cagtagatcg
     5881 cgtgatgacc gaaggcggca ttgccgaccg cgaactggcg gcgctggcgc tgaaacaggc
     5941 cagcggcgat aacgtcgaag cgattttcct gctacgcgcc taccgcacca cgctggcgaa
     6001 gctcgcagta agcgagccgc tcgacaccac ggagatgcgt ctcgaacggc gtatttcagc
     6061 ggtttataaa gatattcccg gcggccagct gcttggccca acctacgact acacccatcg
     6121 cctgctcgat ttcaccctgc tggcaaacgg cgaagcgccg acgctgacca cagccgacag
     6181 cgaacaacag ccgtcgccgc acgttttcag cctgctggcg cgtcaggggc tggcgaagtt
     6241 tgaagaagat agcggcgcac agccggacga catcacccgc acgccgccgg tttacccctg
     6301 ctcacgctcc tcccgcctgc aacaattaat gcgcggcgac gaaggctatt tgctggcgct
     6361 ggcctactcc acccagcgtg gttacggacg caatcatccg ttcgcaggcg aaatccgcag
     6421 cggctatatc gacgtgtcga ttgtgcagga agagctggga tttgcggtga acgtcggcga
     6481 actgctgatg accgaatgtg aaatggtcaa cggttttatc gacccgccgg atgagccgcc
     6541 gcacttcacg cgcggctacg ggctggtgtt cggcatgagc gagcgcaaag cgatggcgat
     6601 ggcgctggtt gaccgcgctc tacaagcccc ggagtacggc gagcacgcga caggcccggc
     6661 gcaggatgaa gagttcgtgc tggcgcatgc cgacaacgtc gaagccgcag gctttgtctc
     6721 gcaccttaaa ctcccgcact acgtcgattt ccaggccgaa ctggagctac tcaaacgtct
     6781 gcaacaggag cagaaccatg gctaatctga gcggctacaa ctttgcctac ctcgacgagc
     6841 agaccaaacg catgatccgc cgcgccatct taaaagcggt ggcgatcccc ggttatcagg
     6901 tgccgtttgg cgggcgcgag atgccgatgc cgtacggctg gggaaccggc ggcatacagc
     6961 ttaccgccag cgtgattggc gaaagcgatg tgctgaaggt gattgaccag ggcgcggacg
     7021 acaccaccaa cgcggtgtcg atccgtaact tctttaagcg cgtgaccggg gtaaacacca
     7081 ctgaacgtac ggacgatgcg acgcttatcc agacgcgcca ccgcatcccc gaaacgccgc
     7141 tgaccgaaga tcagatcatt attttccagg tgccgatccc cgaaccgctg cgctttatcg
     7201 agccgcgcga aacggaaacc cgcaccatgc acgcgctgga ggagtacggc gtgatgcagg
     7261 tgaagctgta tgaagatatc gcccgcttcg gtcatatcgc caccacctac gcctatccgg
     7321 tgaaggtaaa cgggcgctac gtaatggacc cgtcgccgat cccgaaattt gataacccga
     7381 aaatgaacat gatgcccgcc ctgcaactgt tcggcgcggg gcgcgagaag cgcatctatg
     7441 cggtgccgcc gtttacccgc gtggaaagcc tcgatttcga cgatcacccg ttcaccgttc
     7501 agcagtggaa tgagccatgc gccatctgcg gatcgaccca cagctatctt gatgaagtgg
     7561 tgctggatga cgccggaaac cgcatgtttg tctgctccga taccgattat tgccgccaac
     7621 agagcgaggc gaaaagccaa tgaatcaacc gttactttcg gtcaataacc tgacccacct
     7681 ttacgcgccg ggcaaaggct ttagcgatgt ctcttttgat ttatggccgg gggaagtgct
     7741 gggcatcgtc ggggaatccg gctccgggaa gaccacgctg ctgaagtcga tctccgcgcg
     7801 cctgacgccg cagcaagggg aaattcgcta tgaaaaccgt tccctctatg gcatgagcga
     7861 ggccgaccgc cgtcgcctgc tgcgcaccga atggggcgtg gtgcatcagc atccactcga
     7921 cggcctgcgc cgccaggtgt cggcaggcgg caatatcggc gagcggctga tggcgaccgg
     7981 ggcgcgtcat tacggcgata ttcgtgccac cgcgcagaag tggctggaag aggtggaaat
     8041 tcccgccaac cggatcgacg acctgccgac caccttttcc ggcggtatgc agcagcgttt
     8101 gcagattgcc cgcaacctgg tgacgcatcc gaagctggta tttatggacg agccaaccgg
     8161 cgggctggat gtatctgtgc aggcgcgcct gctcgacctg ctgcgcggcc tggtggtgga
     8221 gctgaacctg gcggtggtga ttgtcaccca cgatttaggc gtcgcccgcc tgctggcgga
     8281 ccgtttgctg gtgatgaagc aggggcaagt ggtggagagt gggttaaccg accgcgtgct
     8341 cgacgacccg caccatccgt atacacaact gctggtgtca tcggttttgc agaattgagc
     8401 cggtgccgga tgcggcgtaa acgccttatc cggcctacaa atgcgctccc cgtaggtcgg
     8461 ataagacgcg tcagcgtcgc atccgacacc cgaaccacga ggcgaaaaat gattaacgta
     8521 caaaacgtca gtaaaacctt catcctgcat cagcaaaacg gcgtgcgcct gcccgtctta
     8581 caacgtgcct cgctcaccgt caacgcaggc gaatgcgtgg tgcttcacgg ccactccggc
     8641 agcggaaaat caaccctgct gcgctcgctg tacgccaact atctgcccga cgaagggcaa
     8701 atccagatca aacacggtga ggaatgggta gacctggtca ccgcgccagc ccgcaaagtg
     8761 gtggagatcc gcaaaaccac catcggctgg gtgagccagt ttctgcgcgt catcccgcgt
     8821 atttcggcac tggaagtggt gatgcagcct ctgctcgata ccggcgtacc gcgtgaagcc
     8881 tgcgccgcta aagccgcccg attactgacg cgcctgaacg tgcctgaacg cctgtggcac
     8941 ctggcaccat cgaccttctc cggcggcgaa cagcagcgcg tcaacatcgc ccgtggcttt
     9001 atcgtcgact atccgattct gctgctggat gaacccaccg cctcgctgga cgctaaaaac
     9061 agcgccgcgg tggtggaatt aattcgcgaa gccaaagccc gtggcgcggc catcgtcggt
     9121 atcttccatg acgaagccgt acgcaatgac gtcgccgacc gcctgcaccc aatgggagtc
     9181 tcttcatgat tatcaataac gtcaaactgg tgctggaaaa cgaagtggtg cacggttcac
     9241 tggaagtaca ggatggcaaa attcgcgcct ttgccgaaag ccagagccgc cagccggaag
     9301 cgatggacgg cgaaggtggc tggctgctgc cggggctgat tgagctgcat accgataatc
     9361 tggataaatt cttcaccccg cgtccaaaag tggactggcc cgcccactcg gcaatgagta
     9421 gccacgacgc gctgatggtg gcgagcggca tcaccaccgt gctggacgcg gtggcgattg
     9481 gcgacgtgcg cgacggcggc gatcggctgg agaatctgga gaagatgatc aacgccatcg
     9541 aagagacgca gaaacgcggc gtcaaccgcg ccgagcaccg tctgcatctg cgctgcgaac
     9601 tgccgcatca caccacgctg ccgctgtttg aaaaactggt gcagcgcgaa ccggtgacgc
     9661 tggtgtcgct gatggatcac tcgccgggcc agcgccagtt cgctaaccgc gagaagtatc
     9721 gcgaatatta tcagggcaaa tactcgctca ccgaagcgca gatgcagcag tatgaagaag
     9781 agcagctggc gctcgccgca cgctggtcgc agccaaatcg cgaaaccatc gccgcaatgt
     9841 gccgcgcgcg acacattgcg ctcgccagcc acgatgacgc cacgcacgcc cacgttgctg
     9901 aatctcacca acttggcagc gtaatcgccg aatttcccac cacgttcgaa gcggcagaag
     9961 cctcgcgcaa gcatggcatg aacgtgctga tgggcgcacc gaatatcgtg cgcggcggtt
    10021 cgcactccgg caacgtggcg gccagtgaac tggcgcagct tggcctgctg gatatcctct
    10081 cttccgacta ctaccccgcc agcctgctcg atgcggcatt tcgcgtcgcc gatgacgaga
    10141 gcaaccgctt tacgctgccg caggcggtga agctggtgac taaaaatccg gcgcaggcgc
    10201 ttaatcttca ggatcgcggg gtgattggcg agggcaaacg cgccgacctg gtgctggcgc
    10261 atcgcaaggg caatcacatt catatcgacc acgtctggcg tcagggtaaa agggtgttct
    10321 gatgacggga aaactgattt ggttaatggg ggcctctggc tccggaaaag acagtctgtt
    10381 gacggaactc cgccagcggg aacaaactca gctactggta gcgcatcgct acatcacgcg
    10441 cgccgccagc gccggaagtg aaaaccatat cgccctgagc gagcaggagt tttttacccg
    10501 cgcggggcag aaccttctgg ccttaagctg gcacgctaac ggtctgtatt atggcgtcgg
    10561 cgtcgagatt gacctctggc tgcacgctgg atttgacgtg gtggtcaacg gctcacgcgc
    10621 ccatctgccg caggcgcggg cgcgctatca atcggcgctg ctgcccgttt gtttacaggt
    10681 ttcgccagag atcctgcgcc agcgcctgga aaaccgtggt cgtgaaaatg ccagtgaaat
    10741 taacgcccgg ctggcgcgcg ctgcccgcta tactcctcag gattgtctta cgctcaataa
    10801 tgacggcagc ctgcgccagt cggtcgacaa gttgctgacg ctgattcatc agaaggagaa
    10861 acaccatgcc tgcttgtgag cttcgcccgg ctacgcagta cgacaccgac gcagtttacg
    10921 cgctgatttg tgagctaaaa caggcggagt ttgaccacca ggcgtttcgc gtggggttta
    10981 acgccaatct gcgcgatccg aacatgcgct accaactggc gctgctggat ggcgaagttg
    11041 tcggcatgat cggcctgcat ttgcagtttc atctgcatca tgtcaactgg atcggcgaaa
    11101 ttcaggaact ggtggtgatg ccgcaggcgc gcggtctgaa tgtcggcagc aaattactgg
    11161 catgggcaga agaagaagcc cgccaggccg gggccgaaat gactgagctt tccaccaacg
    11221 tgaagcgcca cgacgcgcac cgattttacc tgcgcgaagg ctacgagcag agccacttcc
    11281 gcttcaccaa ggtgctgtaa catgagcctg accctcacac ttactggcac tggcggcgca
    11341 cagggcgttc ccgcatgggg ctgtgagtgc gtggcctgcg tcagagcgcg gcgctcaccg
    11401 cagtatcgcc gccagccatg cagcggcgtc gtgaagttta acgacgcgat caccctgatc
    11461 gacgccgggc ggcacgatct cgccgatcgc tggtcgcccg gatcgttcca gcagtttttg
    11521 ctgacgcact accatatgga tcacgtgcag gggctgtttc cgctgcgctg gggcgtgggc
    11581 gatcagatcc cggtctacgg cccgccggat gaacagggct gcgacgatct attcaaacat
    11641 tcgggcctgc ttgatttcag ccacacggtg gagccatttg tgatgtttga tttgcaggga
    11701 ttacaggtca cgccgctacc gctcaaccac tcaaaactca ctttcggcta tttactggaa
    11761 acggcgcaca gccgggtggc gtggctatct gacaccgcag gcctgccgga aaaaacgctg
    11821 aaatttttac gcaataatca gccgcaggta atggtgattg attgcagcca cccgccgcgc
    11881 gaggatgcac cgcgtaatca ctgtgattta aataccgtgc ttgcgctgaa tcaggttatc
    11941 cgctcgccgc gggtgattct gacccatatc agccatcagt ttgatgcgtg gctgatggaa
    12001 aacgcgctac catcggggtt tgaggcgggg tttgatggga tggagattga ggtggcgtga
    12061 gggaaaatac ccgcgccggc ccctatatca acgcccatac tcatcttcca ttctccgctc
    12121 ttcatcttcc agttgccgac gctcctgatc aagctggcgc tggcgatcgt ccagctgcct
    12181 gcggcgatct tcaaactggc ggcggcggtc atcatattgt ctgcgccgat cgtcgctcac
    12241 ttcacgctgc cagccgtcgt cgcgcgaatc ttcatagtct cgcccacggt cagggttata
    12301 agcgtcgtta atcgcctgct gaatattgcc aatggtgtcg tcgataatat cggcctgggc
    12361 cggaacggtg gacagcgtga gcagggtgaa taaaagaaat agcggaaagc gtttcattag
    12421 ccaacctcaa aaagaaactc tatccacatt agtcattact catccatgca agtagtggac
    12481 gaatctcaat ttctccgctg ctctattgcc gtaatcgcct ccacgcgttg ttgatgacga
    12541 ccgccttcgt actgtgcgcc cagccacgca tcgacaatca tttttgccag ttcgaggcca
    12601 accactcgtg aaccaaaagc cagcacgttg gtgtcgttat gctgccgcga aagttgcgcg
    12661 gaataaggtt cgctacaaac gaccgcgcga attccggcaa acttgttcgc cgctatcgaa
    12721 ataccgacgc cagtgccaca aatcaaaatc ccgccatcag cctctccgcc agcaacggcc
    12781 agtgcgacgt gactggcgta atgcggataa tcagtacgct ctgacgacca ggttccttta
    12841 tcaatcactt caacgccacg ctcaactaaa tgtgccacta tttcatgttt taaaatgaaa
    12901 ccgacatgat cacagccaaa tgcaatcttt ttcattcatc ttccccgccc gcgcaaaact
    12961 tcacaatcaa aaaacatttg tgatgtaaat gaattaaaaa cacccaaaat cgatgaaatc
    13021 cgccttcccg atctgtttta cagagttaca tctggcagcg caatagcgtt aaagacactc
    13081 actatccagc aacatctatc atctaataaa ccaggaaaac aaataacatc atgtttttaa
    13141 agtgattaaa tgaaatgaaa t
//
LOCUS       DAZKJR010000135        13095 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKJR010000135 DAZKJR010000000
VERSION     DAZKJR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.136"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..879
                     /gene="ydiY"
                     /locus_tag="ACHOWS_004076"
     CDS             121..879
                     /gene="ydiY"
                     /locus_tag="ACHOWS_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFQ0049575.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(932..1039)
                     /gene="yniD"
                     /locus_tag="ACHOWS_004077"
     CDS             complement(932..1039)
                     /gene="yniD"
                     /locus_tag="ACHOWS_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFQ0049576.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1206..2236)
                     /locus_tag="ACHOWS_004078"
                     /pseudo
     CDS             complement(1206..2236)
                     /locus_tag="ACHOWS_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2759..4687
                     /gene="thrS"
                     /locus_tag="ACHOWS_004079"
     CDS             2759..4687
                     /gene="thrS"
                     /locus_tag="ACHOWS_004079"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0049577.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4691..5233
                     /gene="infC"
                     /locus_tag="ACHOWS_004080"
     CDS             4691..5233
                     /gene="infC"
                     /locus_tag="ACHOWS_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0049578.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            5330..5527
                     /gene="rpmI"
                     /locus_tag="ACHOWS_004081"
     CDS             5330..5527
                     /gene="rpmI"
                     /locus_tag="ACHOWS_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0049579.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5581..5937
                     /gene="rplT"
                     /locus_tag="ACHOWS_004082"
     CDS             5581..5937
                     /gene="rplT"
                     /locus_tag="ACHOWS_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0049580.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    6054..6178
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6060..6104
                     /gene="pheM"
                     /locus_tag="ACHOWS_004083"
     CDS             6060..6104
                     /gene="pheM"
                     /locus_tag="ACHOWS_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFQ0049581.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6388..7371
                     /gene="pheS"
                     /locus_tag="ACHOWS_004084"
     CDS             6388..7371
                     /gene="pheS"
                     /locus_tag="ACHOWS_004084"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0049582.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7386..9773
                     /gene="pheT"
                     /locus_tag="ACHOWS_004085"
     CDS             7386..9773
                     /gene="pheT"
                     /locus_tag="ACHOWS_004085"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0049583.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVIECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGAAIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9778..10077
                     /gene="ihfA"
                     /locus_tag="ACHOWS_004086"
     CDS             9778..10077
                     /gene="ihfA"
                     /locus_tag="ACHOWS_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFQ0049584.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            10178..11158
                     /gene="btuC"
                     /locus_tag="ACHOWS_004087"
     CDS             10178..11158
                     /gene="btuC"
                     /locus_tag="ACHOWS_004087"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFQ0049585.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            11221..11772
                     /gene="btuE"
                     /locus_tag="ACHOWS_004088"
     CDS             11221..11772
                     /gene="btuE"
                     /locus_tag="ACHOWS_004088"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFQ0049586.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11772..12521
                     /gene="btuD"
                     /locus_tag="ACHOWS_004089"
     CDS             11772..12521
                     /gene="btuD"
                     /locus_tag="ACHOWS_004089"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFQ0049587.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQHQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLFLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12600..13064
                     /gene="nlpC"
                     /locus_tag="ACHOWS_004090"
     CDS             12600..13064
                     /gene="nlpC"
                     /locus_tag="ACHOWS_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFQ0049588.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         3322 a         3009 c         3461 g         3303 t
ORIGIN      
        1 tgctaagtta ttcgcgcctt tttataattc gttacctttc cagggacgct aatctttgat
       61 ccttaagaaa gtttccggga ccgcgaaggt ctcttttttt gttgtacgga ctcttgataa
      121 atgaagcttt tgaagacagt tcctgccatc gttatgttgg cggggggcat gtttgcgtca
      181 ctgaatgcag ctgccgatga ttccgttttt actgtcatgg acgatcctgc cagcgctaaa
      241 aaacctttcg aaggtaatct gaacgcagga tatcttgcac aatcaggcaa tacgaaaagc
      301 tcctcactta cggctgacac caccatgacc tggtatggac aaaccactgc ctggtcgctg
      361 tgggggaacg ccagtaatac ttcttctaat gatgagcgtt cttcagaaaa atatgcggcg
      421 ggcgggcgta gccgttttaa tttaactgat tatgactatt tatttgggca ggcaagctgg
      481 ctgacagacc gttataacgg ctatcgagag cgcgatgtgt tgaccgcggg ttatggtcgc
      541 cagtttttga atggcccggt acacagtttc cgctttgaat tcggtccagg tgtgcgttac
      601 gacaaatata ccgataatgc cagtgaaacc cagccgctgg gttatgcctc cggggcttat
      661 gcatggcagt tgactgacaa cgctaaattt acgcagggtg tttccgtctt tggcgcggaa
      721 gacacaacgc tgaactctga aagcgcatta aatgttgcta ttaatgaaca ttttggactg
      781 aaggtggctt acaacgtaac atggaactct gaacctccag agtctgcacc ggaacatacc
      841 gatcgtcgga caaccttatc gctgggctac agcatgtaaa attatctgcc ggaccgtgtt
      901 atgcggtctg gctgtcttcg tttgtcactg ctcagcccca aatcattgct gcccaacgta
      961 gcaacaacat agcgccacag attgccagta gcaccaccac catcttccag tgttttttat
     1021 caaagcgttt agtcggcata ccagtgtcct gcatatctct tgtgcgtgat aacggagtgt
     1081 aacaaaaggg acaggcatca agggattttt tgttattaca gccgtcgggt ggcgttgtac
     1141 acctgatcac tgtttttgcg gtttatgaaa aaaataaaat tatgtatcga tatataaata
     1201 tctggttata aatacgtctt tagttcatca gcactaaaac tcaggctttg gctgattacg
     1261 tttatagcag cataatatgt ttctacagtt ttataatgat tatgatgaat ggctatatga
     1321 acagctgaca tgttgtcatg atttttagcg gccaacagtg taaataactg acgttggcta
     1381 aaagaatatt tttttcaaaa gtacccaacg ttgatatata agataacacg acatctttac
     1441 ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg acattgtttg
     1501 ccatcattcg acagaagaaa atatctaaag cgaagccaaa atccttactc gcgataacgc
     1561 gactccctgc aggaggatgg cgaagtaatg atctgaactc cgcgtcccgg actaagggaa
     1621 catgctgctg tatggattca acggaggagt aatgtttaat cagattgact ttgccttttc
     1681 catttctcag tgaatcaagg gcgtattgca atgacagatg gcgacaaaaa atagcgtcag
     1741 aaggagattg cgaaaaaaat gcactaccat tgagatttac tgttggttgt atagtttttt
     1801 ctctatggat ttttttagac agtttcatac aatggttacg attttcggta gttaactgtt
     1861 cctgttcaat ataatccagt atgcttcgca aatggttagc tttaataggc gtggtttcca
     1921 gtaataagga aatgatttct tttttatagt gtaatgtaat gagtatattt tctgcatgga
     1981 tgggtgaagt ggcaaagtta atggtaaaaa gggcagtgga ataacaattt tgtgatattt
     2041 ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcattgaa cttaagtcag
     2101 aggtatcaag tagggtgatg agttggcacg caaattgatt aataggctct gatgaaggcg
     2161 ttggtatgtc gctatcgatg tgcattctat gaggagactt ataatgagtt ctgataataa
     2221 tgtcgttttg actcataatc tattcgcctt atatatgtct ggaggttttg aggatgatga
     2281 ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ctataagaat tgtgtcactt
     2341 ttatcattgt gatatgacca cttcgtcatt aatcttatga tcgtttaaat agtaccgcca
     2401 aattacgcaa cgaactttgc tggattcagt ttatgctgct gcaaatccgc tcgcagtaaa
     2461 cctttcagac gctccgtgat gttatcagtg gttctttaag cgttttgctg gtgtactcac
     2521 tacaaacgaa ttgcgaatcg aatcaatgtg aaacggaaag gtacaatctc ccttttttta
     2581 gtgttaacat cgttcaaccg ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct
     2641 gattgcgaac caatttagca tttgttggct agatggtttc gcaatgaact gttaataaac
     2701 aaatttttct ttgtatgtga tctttcgtgt gggtcaccac tgcaaataag gatataaaat
     2761 gcctgttata actcttcctg atggcagcca acgccattac gaccacgctg taagccccat
     2821 ggatgttgcg ctggacattg gtccaggtct ggcgaaagcc tgtatcgcag ggcgcgttaa
     2881 tggcgaactg gttgatgctt gcgatctgat tgaaaacgac gcacaactgt cgatcattac
     2941 cgccaaagac gaagaaggtc tggagatcat tcgtcactcc tgtgcgcacc tgttagggca
     3001 cgcgattaaa caactttggc cgcataccaa aatggcaatc ggcccggtta ttgacaacgg
     3061 tttttattac gacgttgatc ttgaccgcac gttaacccag gaagatgtcg aagcactcga
     3121 gaagcggatg catgagcttg ctgagaaaaa ctacgacgtc attaagaaga aagtcagctg
     3181 gcacgaagcg cgtgaaactt tcgccaaccg tggtgagagc tacaaagtct ccattcttga
     3241 cgaaaacatc gcccatgatg acaagccagg tctgtacttc catgaagaat atgtcgatat
     3301 gtgccgcggt ccgcacgtac cgaacatgcg tttctgccat catttcaaac taatgaaaac
     3361 ggcaggggct tactggcgtg gcgacagcaa caacaaaatg ttgcaacgta tttacggtac
     3421 ggcgtgggca gacaaaaaag cgcttaacgc ttacctgcag cgcctggaag aagccgcgaa
     3481 acgcgaccac cgtaaaatcg gtaaacagct cgacctgtac catatgcagg aagaagcacc
     3541 gggtatggta ttctggcaca acgatggctg gaccatcttc cgtgaactgg aagtgtttgt
     3601 tcgttctaaa ctgaaagagt accagtatca ggaagttaaa ggtccgttca tgatggaccg
     3661 tgtcctgtgg gaaaaaaccg gtcactggga caactacaaa gatgcaatgt tcaccacgtc
     3721 ttctgagaac cgtgaatact gcattaagcc gatgaactgc ccgggtcacg tacaaatttt
     3781 caaccagggg ctgaagtcgt atcgcgattt gccgctgcgt atggccgagt ttggtagctg
     3841 ccaccgtaac gagccgtcag gttcgctgca tggcctgatg cgtgtacgtg gttttaccca
     3901 ggatgacgcg catatcttct gtactgaaga acaaattcgc gatgaagtta acggatgtat
     3961 ccgtttagtc tatgatatgt acagcacttt tggcttcgag aagatcgtcg tcaaactctc
     4021 cactcgtcct gaaaaacgta ttggcagcga cgaaatgtgg gatcgtgctg aggcggacct
     4081 ggcagttgcg ctggaagaaa acaacatccc gtttgaatat caactgggtg aaggcgcttt
     4141 ctacggtccg aaaattgaat ttaccctgta tgactgcctc gatcgtgcat ggcagtgcgg
     4201 tacagtacag ctggacttct ccttgccgtc tcgtctgagc gcctcctatg tgggcgaaga
     4261 caacgagcgt aaggtaccgg taatgattca ccgcgcaatt cttgggtcga tggaacgttt
     4321 catcggtatc ctgaccgaag aattcgctgg tttcttcccg acctggcttg cgccggttca
     4381 ggttgttatc atgaatatta ccgattcaca gtctgattac gttaacgaat tgacgcaaaa
     4441 actatcaaat gcgggcattc gtgttaaagc agacttgaga aatgagaaga ttggctttaa
     4501 aatccgcgag cacactttgc gtcgcgtccc gtatatgctg gtctgtggtg ataaagaggt
     4561 ggaatcaggc aaagttgccg ttcgcacccg ccgtggtaaa gacctgggaa gcatggacgt
     4621 aaatgaagtg atcgagaagc tgcaacaaga gattcgcagc cgcagtctta aacaattgga
     4681 ggaataaggt attaaaggcg gaaaacgagt tcaaacggcg cgccctaacc gtatcaatgg
     4741 cgaaattcgc gcccaggaag ttcgcttaac aggtctggaa ggcgagcagc ttggtattgt
     4801 gagtctgaga gaagctctgg agaaagcaga agaagccgga gtagacttag tcgagatcag
     4861 ccctaacgcc gagccgccgg tttgtcgtat aatggattac ggcaaattcc tctatgaaaa
     4921 gagcaagtct tctaaggaac agaagaaaaa gcaaaaagtt atccaggtta aggaaattaa
     4981 attccgtcct ggtacagatg aaggcgacta tcaggtaaaa ctccgcagcc tgattcgctt
     5041 tctcgaagag ggtgataaag ccaaaatcac gctgcgtttc cgcggtcgtg agatggcgca
     5101 ccagcaaatc ggtatggaag tgcttaatcg cgtgaaagac gatttgcaag aactggcagt
     5161 ggtcgaatcc ttcccaacga agatcgaagg ccgccagatg atcatggtgc tcgctcctaa
     5221 gaagaaacag taaggccttc aagtagcact gtctgtggag cctttgggct tcacaggcat
     5281 tgttcgcctt tgtttcgttt attaacaatg cgaagtggaa gttattaaaa tgccaaaaat
     5341 taagaccgta cgcggtgctg ctaagcgctt caaaaaaacc ggtaaaggtg gttttaagca
     5401 taagcacgct aacctgcgtc acattctgac caaaaaagcg accaaacgta aacgtcacct
     5461 gcgtccgaaa gccatggttt ccaaaggcga tctgggcctg gtaatcgcgt gcctgccgta
     5521 cgcataagcc gttaacgttt tttaactttt taattagaat atagatacag gagagcacat
     5581 atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa
     5641 gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca ggctgttatc
     5701 aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg
     5761 tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag caaattcatc
     5821 aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga tatcgcagta
     5881 ttcgacaaag tagcgttcac cgctctggtt gaaaaagcga aagcagctct ggcataagcc
     5941 agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa cattgacttt
     6001 tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa
     6061 tgaatgctgc tattttccgc ttcttttttt actttagcac ctgactccag gaggctagcg
     6121 cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct ttttttgtat
     6181 gcgcgttttg aaaaataaaa agcctgtctc atcaggcgta attgtcgcag ccagtctgga
     6241 cacggccagc gcggagaaac cggagcgtac ttaagtacgt gaggatttcg agcactgccc
     6301 gggaccaaaa tggcaagtaa aatagcctga tgggatgggc tctaagtcca acgaaccagt
     6361 gtcaccactg acacaatgag gaaaaacatg tcacatctcg cagaactggt tgccagtgcg
     6421 aaggcggcca ttagccaggc gtcagatgtt gccgcgttag ataatgtgcg cgtcgaatat
     6481 ttgggtaaaa aagggcactt aacccttcag atgacgaccc tgcgtgagct gccgccagaa
     6541 gagcgtccgg cagctggtgc ggttatcaac gaagcgaaag agcaggttca gcaggcgctg
     6601 aatgcgcgta aagcggaact ggaaagcgct gcactgaatg cgcgtctggc ggcggaaacg
     6661 attgatgtct ccctgccggg tcgtcgcatt gaaaacggcg ggctgcatcc ggtgacccgt
     6721 accatcgacc gtatcgaaag tttcttcggt gagcttggct ttaccgtggc aaccgggccg
     6781 gaaatcgaag acgattatca taacttcgat gctctgaaca ttcctggtca ccacccggcg
     6841 cgcgctgacc acgacacttt ctggtttgac gctacccgcc tgctgcgtac ccagacctct
     6901 ggcgtacaga tccgcaccat gaaagctcag cagccaccga ttcgtatcat cgcgcctggc
     6961 cgcgtttatc gtaacgacta cgaccagact cacacgccga tgttccatca gatggaaggt
     7021 ctgattgttg ataccaacat cagctttacc aacctgaaag gcacgctgca cgacttcctg
     7081 cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc cttcctactt cccgtttacc
     7141 gaaccttctg cagaagtgga cgtcatgggt aaaaacggta aatggctgga agtactgggc
     7201 tgcgggatgg tgcatccgaa cgtgctgcgt aacgttggca tcgacccgga agtttactct
     7261 ggtttcgcct tcgggatggg gatggagcgt ctgaccatgt tgcgttacgg cgtcaccgac
     7321 ctgcgttcat tcttcgaaaa cgatctgcgt ttcctcaaac agtttaaata aggcaggaat
     7381 agattatgaa attcagtgaa ctgtggttac gcgaatgggt gaacccggcg attgatagcg
     7441 atgcgctggc aaatcaaatc actatggcgg gcctggaagt tgacggtgta gaaccggttg
     7501 ccggtagctt ccacggcgtg gtcgttggtg aagtgattga gtgtgcgcag catccgaacg
     7561 ctgacaaact gcgtgtgaca aaagtgaatg tcggcggcga tcgcctgctg gacatcgtct
     7621 gcggtgcgcc aaactgccgt cagggcctgc gtgtggcggt agcgaccatt ggtgctgttc
     7681 tgccgggcga tttcaaaatt aaagcggcga aactgcgtgg cgaaccgtct gaagggatgc
     7741 tgtgctcctt ttctgagctg ggaatttctg acgatcacag cggcattatc gagctgcctg
     7801 cagatgcgcc gattggcacc gacatccgcg aatacctgaa actcgatgac aacaccatcg
     7861 aaatcagcgt aacgccaaac cgtgccgatt gcttaggcat tatcggcgtg gcgcgtgatg
     7921 ttgccgtgct gaaccagctg ccgctggttg aaccggaaat cgttccggtt ggtgcggcca
     7981 tcgacgacac gctgccgatt acagtcgaag cgccggaagc ctgcccgcgc tatcttggcc
     8041 gtgtggtaaa aggcattaac gttaaagcgc caactccgct gtggatgaaa gaaaaactgc
     8101 gtcgttgcgg gatccgttct atcgatgcag ttgttgacgt caccaactat gtgctgctcg
     8161 aactgggcca gccgatgcac gctttcgata aagatcgcat tgaaggcggc attgtggtgc
     8221 ggatggcgaa agagggcgaa actctggtgc tgcttgatgg cactgaagcg aagctgaatg
     8281 ctgacaccct ggtcatcgcc gaccacaaca aggcgctggc gatgggcggc atctttggtg
     8341 gcgaacactc tggcgtgaat gacgaaacgc aaaacgtcct gctggaatgc gctttcttca
     8401 gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct gcatactgat gcgtctcacc
     8461 gttatgagcg tggcgttgat ccggcactgc agcacaaagc gatggaacgt gcgacccgtc
     8521 tgctgatcga catctgcggt ggtgaggctg gtccggtaat tgatatcacc aacgaagcaa
     8581 cgctgccgaa gcgtgccacc attactttac gtcgcagcaa actggatcgc ctgatcggcc
     8641 atcatattgc tgatgagcag gtaactgaca ttctgcgtcg tctcggctgc gaagtgaccg
     8701 aaggcaaaga cgaatggcag gccgttgcgc cgagctggcg tttcgacatg gagattgaag
     8761 aagatctggt cgaagaagtc gcgcgtgttt acggctacaa caacatcccg gatgagccgg
     8821 tacaggcaag cctgattatg ggtactcacc gcgaagccga tctgtcgctc aagcgcgtga
     8881 aaacgctgct caacgacaaa ggctatcagg aagtgatcac ctacagcttc gttgatccga
     8941 aagtgcagca gatgatccat ccaggcgttg aagccttact gctgccaagc cctatttccg
     9001 ttgaaatgtc agcaatgcgt ctttccctgt ggaccggcct gctggcaacc gtggtgtaca
     9061 accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag tggtctgcgt ttcgtaccag
     9121 atactcaggc accgttgggc attcgtcagg atctgatgtt agccggtgtg atttgcggta
     9181 accgttacga agagcactgg aacctggcaa aagagaccgt tgatttctat gatttgaaag
     9241 gcgatcttga atccgttctc gacctgaccg gtaaactgaa tgaggttgag ttccgtgcag
     9301 aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat ttatctgaaa ggtgaacgta
     9361 ttggttttgt tggggttgtt catcctgaac tggaacgtaa actggatctt aacggtcgca
     9421 ctctggtgtt cgaactggag tggaacaagc tcgcagaccg cgtggtgcct caggcgcgcg
     9481 agatttctcg cttcccggcg aaccgtcgtg acatcgcggt ggtggtcgca gaaaacgttc
     9541 ccgcagcgga tattttatcc gaatgtaaga aagttggcgt aaatcaggta gttggcgtaa
     9601 acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg gtataagagc ctcgccataa
     9661 gcctgatcct gcaagatacc agccgtacac tcgaagaaga ggagattgcc gctaccgtcg
     9721 ccaaatgtgt agaggcatta aaagagcgat tccaggcatc attgagggat tgaacctatg
     9781 gcgcttacaa aagctgaaat gtcagaatat ctgtttgata agcttgggct tagcaagcgg
     9841 gatgccaaag aactggttga actgtttttc gaagagatcc gtcgcgctct ggaaaacggc
     9901 gaacaggtga aactctctgg ttttggtaac ttcgatctgc gtgataagaa tcaacgcccg
     9961 ggacgtaacc cgaaaacggg cgaggatatt cccattacag cacggcgcgt ggtgaccttc
    10021 agacccgggc agaagttaaa aagccgggtc gaaaacgctt cgcccaaaga cgagtaatct
    10081 gatctaacta aaaaggccgc tctgcggcct tttttctttt cactgtcgaa gagtcaccgt
    10141 aaaatcaacg ccatgacact tcagcagaac ggataccatg ctgacacttg cccgccaaca
    10201 acagcgacaa aatattcgct ggttattatg cctgtcagtt ttgatgctgc tggcgcttct
    10261 cttaagcctt tgcgccggtg aactatggat ctcgccaggt gactggtttt ctcctcgtgg
    10321 cgaactgttc gtctggcaaa ttcgcctgcc acgtacgctg gctgtattgc tggttggtgc
    10381 ggcgctggct atatccggcg ctgtaatgca ggcgttgttt gaaaatcctc tggcagaacc
    10441 tggactactt ggcgtctcta acggcgcagg cgtggggctt atcgccgcgg tattgcttgg
    10501 gcaagggcaa ctccccaact gggcgctagg gctgtgtgcg attgctggcg cgcttatcat
    10561 cactttaata ctcttacgtt tcgctcgtcg tcatctttcg accagtcggt tattgctggc
    10621 tggcgttgca ttagggatta tctgtagcgc actaatgacg tgggctatct acttttccac
    10681 ctctgttgat ttacgtcagc tgatgtactg gatgatgggc ggttttggcg gcgtagactg
    10741 gcggcaaagc tggctgatgc tggcattgat ccccgtgttg ttgtggatct gttgtcagtc
    10801 caggccgatg aatatgttag cacttggcga gatctcggcg cggcaactgg gtttacccct
    10861 gtggttctgg cgcaatgtgt tggtcgcagc gaccggctgg atggttggcg tcagtgtggc
    10921 gctggcgggt gctatcggct ttattggtct ggtgatccca catattctcc ggctgtgtgg
    10981 tttaaccgat catcgcgtat tacttcccgg ctgcgcgctg gcaggggcga gcgcattgct
    11041 gctggccgat attgtagcgc gcctggcatt agctgccgca gagctgccta ttggcgtggt
    11101 caccgcaacg ttgggtgcgc cggtgtttat ctggttattg ttaaaagcag gacgttagcc
    11161 gcaaaaagac ggtctatgat taaaagctaa gatttttact gaccacaccc aggagaaacg
    11221 atgcaagatt ccattctgac gaccgtagtg aaagatatcg acggtgaagt gaccacgctg
    11281 gagaagtacg ccggtaatgt gctgttgatt gtcaatgtcg cctcaaagtg tggcttaacg
    11341 ccgcaatatg agcagttgga gaatattcag aaagcctggg ccgatcgtgg ttttgtggtg
    11401 ctgggattcc cgtgcaacca gtttctggaa caagaaccgg gcagcgatga agagattaaa
    11461 acttactgta ccaccacatg gggggtgacg ttcccgatgt tcagtaagat tgaagttaat
    11521 ggcgaaggac gccatccgct gtatcaaaaa ttgattgccg cagcgccgac cgcagtcgcg
    11581 ccggaagaga gcggattcta tgctcgtatg gtcagcaaag gtcgtgcacc gctgtaccct
    11641 gatgatattt tatggaattt tgagaaattt ttagttggca gggacggatt ggtcatccag
    11701 cgtttttccc cggatatgac gccggaagat cccattgtga tggaaagcat taaactggcg
    11761 ttggcaaaat aatgtctatt gtgatgcagt tacaggatgt tgcggaatct acccgcctgg
    11821 ggccgctttc tggcgaggtt cgggctgggg agatcctgca cctggtgggg ccgaatggcg
    11881 cgggtaagag taccttactg gcgcgaatgg ccggaatgac cagcggtaag ggaagcattc
    11941 agttcgcggg gcaaccactg gaagcatggt ccgcaacaaa actcgcgcta catcgcgcct
    12001 atctttcaca acaccagacg ccaccgtttg caatgccggt ctggcactac ctgacactgc
    12061 atcagcacga taaaacgcgt accgaactac tgaatgatgt cgcaggggcg ctggctcttg
    12121 atgacaaact cggacggagc accaatcaac tttccggcgg tgaatggcaa cgcgtacgtc
    12181 ttgctgcggt ggtgttgcaa atcacaccac aagccaatcc cgcaggccaa ttgctgtttc
    12241 ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag tgcgttagac aaaattctga
    12301 gcgcactgtg tcagcaagga ctggcgattg tgatgagcag tcacgatctc aaccacacct
    12361 tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa aatgctggcc agtggacgca
    12421 gggaagaggt gctcacgccg ccaaatctgg cgcaggccta tgggatgaat tttcgccgtc
    12481 tggatatcga aggtcacaga atgctgattt cgaccatctg atttcattaa tatcttgaaa
    12541 atcgggtaat tacgacgcta aattaatgca agaataaaaa aacagaggaa ccgtacggaa
    12601 tgcgtttctg ccttattttg atcacagcac tgtttctggc cgggtgtagc caccataaag
    12661 caccgccgcc aaatgccaga ctttctgatt cgattaccgt tattgccggt ttgaacgacc
    12721 agctacaaag ctggcatggc acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg
    12781 actgttcggg gtttgtggtt gtgacgatgc gcgatcgttt cgatttgcag ctgccccgtg
    12841 aaaccaaaca acaagcctct atcggcacgc aaattgataa agacgagttg ctgcctggtg
    12901 acctggtctt tttcaaaacg ggctccggac aaaatggttt gcatgtaggt atttatgata
    12961 ccaacaacca atttatccac gcctctacca gcaagggagt gatgcgttcc tcacttgata
    13021 atgtctattg gcagaaaaat ttctggcagg cgagacgaat ctagtatgtc gaaaaatgga
    13081 cggcgatgat acatc
//
LOCUS       DAZKJR010000136        12892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKJR010000136 DAZKJR010000000
VERSION     DAZKJR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.137"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..746
                     /gene="ecpR"
                     /locus_tag="ACHOWS_004091"
                     /gene_synonym="matA"
     CDS             204..746
                     /gene="ecpR"
                     /locus_tag="ACHOWS_004091"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFQ0049589.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            820..1407
                     /gene="ecpA"
                     /locus_tag="ACHOWS_004092"
                     /gene_synonym="matB"
     CDS             820..1407
                     /gene="ecpA"
                     /locus_tag="ACHOWS_004092"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFQ0049590.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1464..2132
                     /gene="ecpB"
                     /locus_tag="ACHOWS_004093"
                     /gene_synonym="yagY"
     CDS             1464..2132
                     /gene="ecpB"
                     /locus_tag="ACHOWS_004093"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFQ0049591.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2158..4683
                     /gene="ecpC"
                     /locus_tag="ACHOWS_004094"
                     /gene_synonym="yagX"
     CDS             2158..4683
                     /gene="ecpC"
                     /locus_tag="ACHOWS_004094"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFQ0049592.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4673..6316
                     /gene="ecpD"
                     /locus_tag="ACHOWS_004095"
     CDS             4673..6316
                     /gene="ecpD"
                     /locus_tag="ACHOWS_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFQ0049593.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6285..6995
                     /gene="ecpE"
                     /locus_tag="ACHOWS_004096"
     CDS             6285..6995
                     /gene="ecpE"
                     /locus_tag="ACHOWS_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFQ0049594.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQTGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7308..7637
                     /gene="ykgJ"
                     /locus_tag="ACHOWS_004097"
     CDS             7308..7637
                     /gene="ykgJ"
                     /locus_tag="ACHOWS_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFQ0049595.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7649..7815)
                     /locus_tag="ACHOWS_004098"
                     /pseudo
     CDS             complement(7649..7815)
                     /locus_tag="ACHOWS_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7886..8500)
                     /gene="yagU"
                     /locus_tag="ACHOWS_004099"
     CDS             complement(7886..8500)
                     /gene="yagU"
                     /locus_tag="ACHOWS_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFQ0049596.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8715..8900)
                     /locus_tag="ACHOWS_004100"
     CDS             complement(8715..8900)
                     /locus_tag="ACHOWS_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049597.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            8949..9106
                     /locus_tag="ACHOWS_004101"
                     /pseudo
     CDS             8949..9106
                     /locus_tag="ACHOWS_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835868.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(9121..9196)
                     /locus_tag="ACHOWS_004102"
     tRNA            complement(9121..9196)
                     /locus_tag="ACHOWS_004102"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9161..9163),aa:Thr,seq:cgt)
     gene            complement(9311..10564)
                     /gene="proA"
                     /locus_tag="ACHOWS_004103"
     CDS             complement(9311..10564)
                     /gene="proA"
                     /locus_tag="ACHOWS_004103"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0049598.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKTEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     SRGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(10576..11679)
                     /gene="proB"
                     /locus_tag="ACHOWS_004104"
     CDS             complement(10576..11679)
                     /gene="proB"
                     /locus_tag="ACHOWS_004104"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0049599.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            11968..>12892
                     /gene="phoE"
                     /locus_tag="ACHOWS_004105"
     CDS             11968..>12892
                     /gene="phoE"
                     /locus_tag="ACHOWS_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFQ0049600.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDV"
BASE COUNT         3376 a         3305 c         3197 g         3014 t
ORIGIN      
        1 acactcatca ggaaaaaaac ataaatttaa acctaatcga aataattaaa acttaatctc
       61 gtttaaccta tattgatatg tgctacgtat cttatttact tccgatttac taaagaaact
      121 gaatgtacct gtaaaaatta caggtttgga aagtagtgac atggcaaaat gattacagca
      181 gggactatga ggttaaaaac catatggaat gtcaaaaccg ttctgataaa tacatctggt
      241 ctccccatga cgcctacttc tataaaggac tatctgaact gattgtggat atcgacagat
      301 taatttatct atcgctggag aaaatcagaa aagatttcgt gtttatcaat ctcaatacgg
      361 attctttaac tgagtttata aaccgtgata atgagtggtt atccgcggta aaggggaaac
      421 aggtcgtatt gattgcggcc agaaagtcag aagccttagc aaattattgg tattacaaca
      481 gcaatattag gggcgtggta tacgctggac tgagtcgtga tattagaaaa gaactggcct
      541 atgtgattaa tggcaggttc ctgagaaaag atattaagaa agataaaatc actgaccggg
      601 aaatggaaat tatccgcatg acggctcagg gaatgctgcc taaatcgatt gccagaattg
      661 aaaattgtag tgtgaagaca gtgtataccc atcggcggaa tgcagaggcc aagctgtact
      721 caaaattata taagttggtt cagtaaactc caagcaagtt agttttaaaa atgactcacc
      781 gggacttcat gtcctcaatt caactcggga agaaaagcaa tgaaaaaaaa ggttctggca
      841 atagctctgg taacggtgtt taccggtaca ggtgtagcgc aggctgctga cgtaacagct
      901 caggctgtag cgacctggtc agcaacagcc aaaaaagaca ccaccagtaa gctggttgtg
      961 acgccactcg gtagcctggc gttccagtat gccgaaggca ttaaaggttt taactcacaa
     1021 aaaggtctat ttgacgtggc tatcgagggt gactcaacgg ctaccgcctt taaactgacc
     1081 tcacgcctta tcaccaacac cttaacccag ttggatacct caggttccac actgaatgtg
     1141 ggcgtggatt ataacggcgc ggcagtcgaa aaaactggcg ataccgtgat gatcgatacc
     1201 gccaacggcg tactgggcgg caaccttagc ccactggcta acggttacaa tgccagcaat
     1261 cgtaccaccg cacaggatgg tttcactttc tccatcatca gcggcaccac caatggtacc
     1321 accgcagtaa ccgattacag cactctaccg gaaggcatct ggagcggcga cgttagcgta
     1381 cagttcgacg ctacctggac cagttaatct ctctgatgta ccagcagggg tacccccctg
     1441 ctttattcct ggatggaatt gttatgaaaa agcaccttct gcctctcgct ctgctgtttt
     1501 ccggaatatc accggcccag gcgctggatg tcggcgatat atcatcgttt atgaacagtg
     1561 acagcagcac gctaagcaaa acgatccaaa acagtaccga cagtggccgc ctcatcaata
     1621 tccgtctcga acggctctct tcaccgcttg acgacgggca ggttatcgca atggacaagc
     1681 cggatgagtt gctactcact cccgccagct tgctgctacc cgcccaagcc agcgaagtga
     1741 tccgcttctt ctataaggga ccagcagatg aaaaagagcg ctactaccgc attgtctggt
     1801 ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa ccgcagcgct gtggccactg
     1861 cttccgcccg catcggcacc attctggtcg tcgcaccccg ccaggcaaac taccactttc
     1921 agtacgccaa cggctccctg acaaatacag gaaatgcgac gctgcggatc ctcgcctacg
     1981 gcccctgcct gaaagccgcc aatggtaagg agtgtaaaga gaattactac ctgatgccgg
     2041 gcaagtcgcg tcgttttacc cgcgtggaca cggcggataa caaaggacgg gttgcacttt
     2101 ggcagggtga taagttcatt cccgtgaaat agatagctgt gcagatggat aacgacaatg
     2161 cctttacgac ggttctcccc aggactgaaa gcccagtttg ccttcggcat ggtctttttg
     2221 ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc agcaaatagg tggggtgatt
     2281 attccgcagg ccttcagtca ggcgctccag gacggcatga gcgtcccgct ttatattcat
     2341 ctcgccggta gccagggtcg ccaggacgat cagcgaatcg gcagcgcttt tatctggttg
     2401 gatgatggac agttacgcat ccgaaaaata cagctggaag agagtgaaga taacgccagt
     2461 gtcagcgaac aaactcgaca gcagctgatg actctggcca acgccccgtt caatgaggcc
     2521 cttaccatcc ccctgactga caacgcgcag ttggatctca gcttgcgcca actgctgctg
     2581 cagctggtgg tcaagcgcga agcgctgggc accgtactac gctcacgtag cgaagacatc
     2641 gggcagtcca gtgttaacac cctcagcagt aatctgagct ataacttcgg cgtctataac
     2701 aaccagttgc gtaacggcgg gagcaacacc tccagctatc tgtcgctgaa taacgttact
     2761 gcgctgcgcg aacatcacgt ggtgctcgac ggctcactgt acgggattgg tagcggtcaa
     2821 caggacagtg aattatataa agcgatgtat gaacgcgatt ttgctggtca ccgatttgcc
     2881 ggtggaatgc tcgacacctg gaacttgcag tccttagggc cgatgaccgc catttcagca
     2941 gggaagattt acggcctttc ctggggaaac caggccagct ccaccatctt cgacagcagc
     3001 cagtcggcca cgccagtgat cgccttttta ccggcggcgg gcgaagtaca tctcacccgt
     3061 gatgggcggc tactaagcgt tcagaacttc accatgggca atcatgaagt ggatacccgg
     3121 ggtctaccgt acggtattta cgatgtggaa gttgaggtaa tcgttaacgg tcgcgtgatc
     3181 agcaaacgca cccagcgggt caataagctg tttagccggg gacgtggcgt cggtgcaccg
     3241 ctggcgtggc agatatgggg cggtagcttt catatggatc gctggtcgga aaacgggaaa
     3301 aagacgcgac cagccaaaga gagttggctg gcaggtgcct cgacctccgg ctcactgagt
     3361 acgtttagct gggcggcaac gggatatgga tacgataatc aggcggtggg tgaaacccgt
     3421 ctgacgctgc cgcttggggg ggcgatcaac gttaacctgc aaaacatgct ggccagtgac
     3481 agctcatgga gcaacatcgc cagcatcagc gccactctac ctggaggctt cagttcgctg
     3541 tgggttaacc aggaaaaaac ccgcattggc aatcaattgc gacgtagcga tgccgacaac
     3601 cgtgcaatcg gcggcacact caacctgaac tcactgtggt cgaagctggg tacgttcagc
     3661 atcagctaca atgatgaccg ccgctacaac agccattatt acacggcaga ttactatcaa
     3721 agtgtctaca gcggtacctt tggttcgctt ggcctgcggg ccggtattca gcgctataac
     3781 aacggcgaca gcagcgccaa tacagggaaa tatatcgctc tcgatctctc gctaccactg
     3841 ggcaactggt ttagcgcagg gatgacccat caaaacggct acaccatggc aaacctgtca
     3901 gcacgcaaac agtttgatga aggaaccatt cgcactgttg gtgccaatct gtcacgagcc
     3961 atctccggcg ataccggtga tgacaaaacc ctcagcggtg gggcgtatgc acagttcgac
     4021 gctcgttacg ccagcggaac gctgaacgtc aatagcgcgg cggacggcta catcaatact
     4081 aatttgaccg ccaacggcag cgtcggctgg cagggtaaaa acatcgctgc cagcgggcgg
     4141 accgatggca acgctggggt gatattcgac actggcctgg agaacgatgg tcagatcagc
     4201 gccaaaatca acgggcggat tttcccgctt aacggcaagc gtaactatct cccgctctct
     4261 ccctatggaa gatatgaggt ggagttgcag aacagcaaaa actcactcga cagttacgat
     4321 atcgtcagcg gtcgcaaaag tcatctgact ctctatccag gcaatgtcgc tgtcattgag
     4381 ccagaggtga agcagatggt taccgtctcc ggtcgtatcc gtgcggaaga cggcacactg
     4441 ctggctaacg cacggattaa caaccatatc ggccgaaccc gaaccgatga aaacggcgag
     4501 tttgtcatgg acgtggataa gaaatacccc actatcgatt ttcgctacag tggcaataaa
     4561 acctgcgaag tggcactgga actcaaccag gcgcgcggtg ccgtctgggt cggtgatgtg
     4621 gtctgcagcg gcctctcatc gtgggcggcg gtgacgcaga caggagaaga gaatgagagt
     4681 taacctactg atagcgatga taatctttgc gctaatctgg cctgcaaccg cgctcagagc
     4741 ggcagtgagc aaaacaacct gggcggatgc tccggcacgc gagtttgtgt ttgtcgaaaa
     4801 caactcagac gacaactttt tcgtcactcc tggcggggcg ctggatccgc gcctgaccgg
     4861 tgccaaccgc tggaccggtt taaaatacaa tggttcagga accatctatc agcaaagcct
     4921 cggctacatt gataacggtt acaacaccgg cctttatacc aactggaagt ttgatatgtg
     4981 gctggaaaat tcaccagttt catctccttt aactggcttg cgctgcatca actggtacgc
     5041 agggtgtaat atgaccacca gtcttatcct gccgcaaacc accgacgcca gtggatttta
     5101 tggcgcgacg gtgaccagcg gcggcgcgaa atggatgcac ggcatgttgt cagacgcgtt
     5161 ttaccagtat ctgcaacaaa tgcccgtcgg cagcagcttt acaatgacca tcaatgcctg
     5221 ccagacctct gtgaactatg acgccagcag cggcgcacgc tgtaaggatc aggcctccgg
     5281 caactggtat gttcgcaacg tcacccatac gaaagcagca aatctgcggt tgataaatac
     5341 ccactcgctg gcggaagtat ttatcaacag cgacggagta ccgactctgg gcgaagggaa
     5401 cgccgactgc cggacgcaaa ccatcggcag ccgttcagga ttaagttgta agatggttaa
     5461 ctataccctg caaacaaacg gactcagcaa cacctcaatc catatattcc cggcgatcgc
     5521 caactcgtcg ttagcctcgg ccgtcggggc gtacgatatg cagttcagtc tgaatggcag
     5581 ttcatggaaa ccggtgagca atactgccta ttactacacc ttcaacgaga tgaagagcgc
     5641 agactcgatc tatgttttct tctcgagcaa cttctttaaa cagatggtga acctcgggat
     5701 cagcgatatc aacaccaaag atctattcaa ctttcgcttt cagaacacca catcaccgga
     5761 gtctggctgg tatgaatttt ctacctccaa cacgctgatt atcaaacccc gtgatttcag
     5821 catcagtatt atctccgatg aatatactca gacaccgtcg cgggagggat atgttggcag
     5881 cggcgagtcg gcactcgatt tcggctatat cgtaaccacc agcggtaaaa cagctgccga
     5941 cgaagtgctg atcaaggtga ccggacccgc gcaggtgatt ggcgggcgct cctattgtgt
     6001 cttcagctcc gatgacggta aggcgaaagt accgttcccg gcgacgcttt cctttattac
     6061 ccgcaacgga gctacaaaaa cctacgatgc cgggtgcgat gatagctggc gggatatgac
     6121 cgatgcgctg tggttgacca caccgtggac tgatatctct ggcgaagtgg ggcagatgga
     6181 taagaccaca gtcaaatttt cgattccaat ggataacgcc atttctctgc gtacggtaga
     6241 tgataacggc tggtttggcg aagtcagcgc ttcaggagag attcatgttc aggcgacgtg
     6301 gcgtaacatt aactaaggcc ctgctgacag tggtctgtat gctggcggca cctttgacac
     6361 aggcgatttc ggtcggcaat ctgacttttt cactgccgtc cgagactgac tttgtcagca
     6421 aacgtgtcgt gaataacaac aaaagcgcgc ggatataccg tattgccatc agtgctattg
     6481 atagcccggg cagcagtgaa ttgcgcaccc gaccggtgga tggtgaacta cttttcgccc
     6541 cccgccagct ggcgttgcag actggtgaga gcgagtattt taaattttac tatcatggtc
     6601 cacgggataa ccgcgagcgc tactaccggg tctcatttcg cgaggtcccc actcgtaacc
     6661 tgacaaaacg cagcccaacc ggcggtgagg tcagcacgga gccggtggtg gtgatggata
     6721 ccattctggt agtacgacca cgtcaggttc agtttaaatg gtccttcgat caggtgaccg
     6781 gaacggtaag taacaccggc aacacgtggt ttaagctact gattaaacca ggatgtgatt
     6841 cgaccgaaga ggaaggcgat gcctggtatc tacgtccggg agacgtggtt catcagcctg
     6901 agttacgtca gccggggaat cattatctgg tctataacga caaattcatt aagattagcg
     6961 attcctgtcc ggctaagccc ccttcggcgg actaagactt atccatcgga cgagggaaga
     7021 attccacctc agagactcaa aaatcgttta aatgatgtgg aagcgatcgt caatgtcacc
     7081 tgtaacgatg agctttctca tatgcacacc acagtgttca ggagacacgg aaaggtacca
     7141 ggcggcctgc taacgagtgg tttcttcact aattaggacg taaaatcggt aacggctgga
     7201 aatcattcaa tactcacact atcgaaagta caccagccaa ccgcgataca tccctgcata
     7261 cgacgtaccg cagttctctt tacgatttat agctgtactg gtgaattatg agcaatctga
     7321 atccatgcat gacgtgtggt gcctgttgtg catttttccg cgtctctttt tactgggccg
     7381 aagctgacga tgctggcggt accattcccg ccaggctcac tgaacaaata tccccttttc
     7441 accgatgcat gagcggtact aatcagaaaa atccccgatg tattgccctt gcaggaacac
     7501 cgggcaaaaa tgcctgctgc acgatatatg aaaatcgatc gtccacatgc agagaattcg
     7561 ccatgtctgg tgagaacgga gaagtcaatg aggcttgcaa tcgtgcaaga gctaaatacg
     7621 ggctaacacc gttataaata tacaataatt aattgcattg ccccgccagc tgtaatagcg
     7681 gggcttcgtt tttcagggac aacaaaacct gaaacttctt ccattttcca gcaacaatgc
     7741 ctcttcttct gtagcgttct ccggatctac atcatcctga agtagcgcga tatgatcact
     7801 gactgtccgc atcagaatat gaaaagcaac gtttttaacc tataaaaatg gcgctatatt
     7861 tgcgccattt ttatcattca aagcattatc tatttgagcc taaagggatc tcaggatctg
     7921 gctcatgggt aattctgttt cgtaaatctc tgcgaataat ttcaatagac cagaaccaga
     7981 ctaaatgtcc aaaaatttca gagacattct cataccacgg aagatcaaag agaggtggcg
     8041 taagtcccat gagagggaat gaaatcatat gaacaaaaag ttgcgctaaa gcacctgcca
     8101 gtaagccctg ccaaagttta attttcggaa atacttcggc gaccacacag taaccaacgg
     8161 caaaaactat cgaaaagatt atgtgcgtta caccaaccca gttaaagaca tgcccggcga
     8221 aggtataaac agccgaattt ggatctgtca gtcccaacca atcccgaaga aaaatgtacg
     8281 gaggattgag aaagttacgg gagcaatcaa tttggcctgc agccctgatt aatgattccg
     8341 gaccacacgc tgcattaaac atatcaaccg gactacgtgg tggcaatgga acttctgccc
     8401 cccacttaac aaatgcggaa acaacgccag caatcagccc aatgaatgca gcaagaccat
     8461 aacgtctgcg gtttggtgga gtttgttcaa atatattcat atctaccctg cttgtaccat
     8521 tatgttatac acctctttag gagtattcat aaaacaaggc aaatgtaaag aactgtattg
     8581 ttttgtataa ccacatggtt acctaatcgc caattaatat aagctccatt atttctccat
     8641 attttcatgt tagaagtgac aaggagaaat gtaatgtttc tctcagagaa tataaatcat
     8701 tccttttgcc caccttaatg gtgggctttt ttatgcaaaa cctgaacgtc ctgtggtatt
     8761 cctctggcat aatgattttc gcgattaacc aacaaccaga ccagcaaaga cgccaccaca
     8821 agcacagtaa catccatcag gcttgccaca ccccatgcgt gcggctcaaa gccgccatgg
     8881 catattaatc cgcttgccat tttcgaacag ctgattggtt tggtccggtg tttccctgct
     8941 tgcacttaaa aatgagcgga tgggaaactc tcgaaatggt tcgtcgatat gctcacctcg
     9001 cacctaatca ccttaccgaa cacgcacggt aaatagattc gatcctgaac ccatcggtcc
     9061 caaatctgtc ccaataaaaa ataaggaagg tattagtgat gtgtaactta ttgatttaaa
     9121 tggtgccgat aagaggagtc gaacctccga ccttcgcatt acgaatgcgc tgctctacca
     9181 actgagctat atcggccctg aaaggacatg ttcacgaacg tgaatcacgg tggacaaggt
     9241 taaaactaac cgggcgatgc gtcaatggcc ttgtgaatca aatggctact tttgcatcac
     9301 ccggttttat ttacgcacga atggtgtaat caccaatgcc gatccacttg taagtggtca
     9361 gtgcttccag ccccattggg ccacgcgagt ggagtttttg tgtgcttacc gccacttccg
     9421 cacccagtcc aaactggccg ccgtcggtaa aacgcgtaga ggcgttaacg taaacagcgg
     9481 aggaatctac ttcgttaaca aaacgctggg cgttgcgcat atcgcgggtc aggatcgcat
     9541 cggagtgttg cgtgccgtgt tcacgaatat gggcgatggc atcgtcaaga tcgctgacga
     9601 ttttgacgtt caaatctaat gacagaaact catcgtcata ctcttcggtt ttaacagcaa
     9661 ccaccttcgc ggggcctgtc tgcaactgcg ccagtgcacc tgcatcagcg tgtaacgtca
     9721 cgccactttc cgccatttgt ttgcttaatg cgggcaggaa actatcggcg atgtttttat
     9781 tcaccagcaa cgtttcaacc gtattacatg tgctcggtcg ctgagttttc gcgttgacga
     9841 tcactttcag ggcttcagcg atctctgcac tttcatcaac gtaaatatgg catacgccta
     9901 taccacctgt gatcaccggg atcgtcgact gttcgcggca cagtttatgc aggccagcgc
     9961 caccgcgcgg gatcagcatg tcgatgtatt tatccatacg cagcatttca ctgaccagcg
    10021 cgcggtcagg attatcaatc gcctgcacgg cacccgccgg taagctgcag gatttcaggg
    10081 cgtcctgaat caccgccacc gttgcagcgt tagtgcgaca cgtttctttg ccgccgcgca
    10141 ggatcaccgc gttacctgtt ttcaggcaca gcgaagcgac atctaccgtc acgttcgggc
    10201 gcgcttcata aatcacgcca atcaccccca gcggcacgcg acggcgctcc agacgcagac
    10261 cactgtccag tacgccgcca tcgattacct gccccaccgg atcggcgaga ttacacacct
    10321 gacgcacatc gtcggcgatg cctttcagcc gtgcgggcgt cagtgccaga cggtcaagca
    10381 tcgcttcgct aaggccattg gcacgcgcgt cagcaacatc ctgggcgtta gcgttgagga
    10441 tgatttcgct ttgtgcttcc agttcatcgg cgattttttc cagcacacga tttttttcgc
    10501 ggctggagag ttgcgctaat ttatacgagg cttgctttgc ggcaatgccc atttgttcca
    10561 gcatcggcca gctccttaac gggtaatcat gtcatcacgg tgaacggcaa ccgggccata
    10621 ttcatatccc agtattgcat caatttcttg cgagtggtgt ccggcaatgc ggcgtaatgc
    10681 atcgctgttg taacgactga cgccgtgggc gatatcgcga ccttcgaggt tgcaaatgcg
    10741 gatgacttca ccacgcgaga aattgccagt tacgctctta atgcctttcg gcaacaggga
    10801 gctgccgcgt tcaagaatgg cggcggttgc cccttcatct accgtgattt cacccgccgg
    10861 cggcgcaccg aaaatccagc gtttacggtt ttcaagcgga gtcgcctggg catggaacag
    10921 cgtaccgacg gaaatgcctt ccatcacatc accaataacg cccggcttgc tgcccgcggc
    10981 aataatggtg tcgatacccg cacggcaagc cacgtccgcg gcctgcaatt tggtgctcat
    11041 accgccagtt ccgaggcctg aaacgctatc accggcaatc gcgcgcagtg cgtcatcaat
    11101 gccgtacaca tctttaatca gttctgcctg cggattgctg cgcgggtcag cggtatacaa
    11161 acctttttga tcggtcagca gcaacagttt atcggcaccc gccagaatcg ccgccagcgc
    11221 agaaaggtta tcgttatcac cgaccttaat ctctgccgta gcgacagcat cgttctcatt
    11281 aattaccgga acgatattgt tatcgagcaa cgcacgcagg gtatcgcggg cgttcaggaa
    11341 gcgttcacga tcttccatat cagcacgggt cagcaacatc tgcccgacat gaatgccgta
    11401 aattgaaaac agctgttccc acagttgaat cagtcgactc tgccctaccg ccgccagcag
    11461 ctgttttgaa gcgatagtcg ctggcaattc cgggtacccc aggtgctcac gtccggcggc
    11521 aatcgcaccc gacgtcacaa taacaatccg atgcccggcg gcatgtaact gcgcgcactg
    11581 gcgaacaagt tcaacgatat gggcacggtt caggcggcgc gatccgcctg ttagcacact
    11641 ggtgccgagt tttaccacca gcgtctggct gtcactcatg attctctgcc attcaatttt
    11701 aggaaaaaat gatatcaaac gaacgtttta gcaggactgt cgtcggttgc caaccatctg
    11761 cgagcaaagc atggcgtttt gttgcgcggg atcagcaagc gtagcggcag ttgtttacgc
    11821 ttttattaca gatttaataa attaccacat tttaagaata ttattaatct gtaatatatc
    11881 tttaacaatc tcaggttaaa aactttcctg ttttcaacgg gactctcccg ctgaatattc
    11941 gcgcgttaat taaaatcagg aatgaaaatg aaaaagagca ctctggcatt agtggtgatg
    12001 ggcattgtgg catctgtatc cgtacaggcc gcagaaatat ataacaaaga cggtaataaa
    12061 ctggatgtct atggcaaagt taaagccatg cattatatga gtgataatga cagtaaagat
    12121 ggcgaccaga gttatatccg ttttggtttt aaaggcgaaa cacaaattaa cgatcaattg
    12181 accggttatg gtcgctggga agcagagttt gccggtaata aagcagagag tgatactgca
    12241 cagcaaaaaa cacgtctcgc ttttgccggg ttgaaatata aagatttggg ttctttcgat
    12301 tatggtcgta acctgggcgc gttgtatgac gtggaagcct ggaccgatat gttcccggaa
    12361 tttggtggcg actcctcggc gcagaccgac aactttatga ccaaacgcgc cagcggtctg
    12421 gcgacgtatc ggaacaccga cttcttcggc gttatcgatg gcctgaactt aaccctgcaa
    12481 tatcaaggga aaaacgaaaa ccgcgacgtt aaaaagcaaa acggcgatgg cttcggcacg
    12541 tcattgacat atgactttgg tggcagcgat ttcgccatca gtggagccta taccaactct
    12601 gatcgcacca acgagcagaa cctgcaaagc cgtggcacag gcaagcgtgc agaagcatgg
    12661 gcaacaggtc tgaaatacga tgccaataat atttatctgg caactttcta ttctgaaaca
    12721 cgcaaaatga cgccaataac tggcggcttt gccaataaga cgcagaactt tgaagcggtc
    12781 gctcaatacc agtttgactt tggtctgcgt ccatcgctgg gttatgtctt atcgaaaggg
    12841 aaagatattg agggcatcgg tgatgaagat ctggtcaatt atgtcgatgt tg
//
LOCUS       DAZKJR010000137        12834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKJR010000137 DAZKJR010000000
VERSION     DAZKJR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.138"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..174)
                     /locus_tag="ACHOWS_004106"
     CDS             complement(<1..174)
                     /locus_tag="ACHOWS_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049601.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALN"
     gene            complement(270..893)
                     /locus_tag="ACHOWS_004107"
     CDS             complement(270..893)
                     /locus_tag="ACHOWS_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFQ0049602.1"
                     /translation="MRTRIDYLANKYSFTELNESPRLRWQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRQSGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            complement(982..1494)
                     /locus_tag="ACHOWS_004108"
     CDS             complement(982..1494)
                     /locus_tag="ACHOWS_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049603.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHRGATIRCLLLLSQTSICH
                     PRASVTVDEECSQLVDLLQQTWKVISAGGQCRVEKRFQVVRGDTSGQYVALKTVVQSF
                     VLPVMTTINW"
     gene            complement(1776..2012)
                     /locus_tag="ACHOWS_004109"
     CDS             complement(1776..2012)
                     /locus_tag="ACHOWS_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0049604.1"
                     /translation="MLTSITGHDSILLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(2081..2656)
                     /locus_tag="ACHOWS_004110"
     CDS             complement(2081..2656)
                     /locus_tag="ACHOWS_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049605.1"
                     /translation="MNAIPYFDYSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDSCSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            complement(2914..3480)
                     /locus_tag="ACHOWS_004111"
     CDS             complement(2914..3480)
                     /locus_tag="ACHOWS_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFQ0049606.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMLARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWIDRQRVKKIY
                     PFAERIAECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRNLQK"
     gene            complement(5060..6664)
                     /locus_tag="ACHOWS_004112"
     CDS             complement(5060..6664)
                     /locus_tag="ACHOWS_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFQ0049607.1"
                     /translation="MNPDYVQALQTSPHFPTDVTYRLFPSDLAYLIDDLYSSTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMTPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKAVNRGDPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALAEGLNEHPEAGIISDEAVTFFRSYLKNNPGLLNKAWSGQ
                     PFDFGRADEKYHITPRLTFSLMSQPNVFTDYINKNDVLAWGSGFLSRFLFSQVGGSSR
                     VRDYRKGEFVTIPTLVEFHKKINGFLSSHSINSPGMRSERKTLKLSKKALGEWQENQI
                     KIESKALAGGEWEHIRDIVLKAGSNILRIAGLFTCYCYKDDEEITSIALWKAMHLMEW
                     YLEEASTIFYPMSERCQFEQDARELYTWIMTRIKQNNLYAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLIAQNYLCIIKMRSHQALYVSVHCNNRYLLPCGAMSYEQFDIVPPLNSHN
                     AKTYHVNIPLALIPSFALPFSAYTLF"
     gene            complement(7094..7999)
                     /locus_tag="ACHOWS_004113"
     CDS             complement(7094..7999)
                     /locus_tag="ACHOWS_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0049608.1"
                     /translation="MALEQEIANLVNDFVAGGRPSCRDQTFDERRAGYIASTALAGDT
                     ETRVNISDVIIDDILLRIFSPLNDDKLSSAIVYYHGGCFVSGGFVTHDNQFRQLAFLS
                     GCRVIAVQYRLAPEHIYPAAHDDAEHAANIVWQHAEMLKVDRNKITLMGDSAGGHLAL
                     ITALRVKNIAQWLPRKLILIYPMLDATASCDSYKLNGSDYVLTEDTFLSGYEAYFSGL
                     DLEHPEASPLFRKDFNGLPPVHIITAEYDPLRDEGEELFSRMIQQGVKCTAQRYLGVI
                     HGFFQLGGISQTARNLMQDICGIVE"
     gene            complement(8453..8905)
                     /locus_tag="ACHOWS_004114"
     CDS             complement(8453..8905)
                     /locus_tag="ACHOWS_004114"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFQ0049609.1"
                     /translation="MNVIDSGIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILTTKICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
     gene            complement(9419..10150)
                     /locus_tag="ACHOWS_004115"
     CDS             complement(9419..10150)
                     /locus_tag="ACHOWS_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFQ0049610.1"
                     /translation="MNQSIHNAYWLSRFDSLLDSAPAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDIWCSLGDFSEPSSLATMIQQAWCSALHPEPWQGDGLVHFSRWTPSRKPA
                     AADASPSSDDTPLHSGCSDTRTDSDKRPGGSAVLWAKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRGVRCLLDGR"
     gene            10821..11351
                     /locus_tag="ACHOWS_004116"
     CDS             10821..11351
                     /locus_tag="ACHOWS_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFQ0049611.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAAMTITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
BASE COUNT         3816 a         2960 c         2671 g         3387 t
ORIGIN      
        1 gttcagagca tcccaggaaa cgttcataca gaagctgtcc tgcaatttgc aggcaaccac
       61 ctctgccagg cggttccggt tatccgccag tccggcataa cctctctctg ccagccactc
      121 attcagttca tgttccggag acagattgat aatatcgtaa tcggaccttg ctattcccgt
      181 gtccttagca catgttttca ggcacgggga taaagtataa agaatgtcgt actgctgctg
      241 ccagagcaat attccccctg atggtcgtat cagagatggt atgccggtat tttgcgggtt
      301 gttcccgtca ggttatcgct cacttccacg gacgttgtca ccaccggcat tccgctctgt
      361 cttagtctca ggacatcagc cgcaatacgc tgactgccag accagaaaag gccgtccagt
      421 gcggtcacca gtaagcccgc ctccagcgca tacttcagcc gttcacgggg cgctttcact
      481 tccctggcaa cctgctgata tggcgatgat gtgctttcat ttccaaccac gcgacgaata
      541 cggtgagaca gatgataccg gtatgtatcc ggcacaccgg aaaggctggc cttcaggctg
      601 tacacgcagc caaagcgttt atcgttgaac atcacatttt tctggctgat gtcccattct
      661 tcacgcagcg cggcaatcag ttgtggtgta cggataagca acaagcgaaa aggcagttca
      721 aaactggtga cgtaatccac attcagcaga gcaatacgca gccgttcttc tggcccggcc
      781 tctgtctgcc ggcactcctc cagaacatcc tgccactgcc agcgaagacg gggggattca
      841 ttcagctctg taaaactgta tttattcgcc agataatcaa ttcgtgtacg catactgaaa
      901 agaattctgt ataaaaattc atctgacaaa actttatcag tccataagaa tcaacggaag
      961 cgcagctggt tcacacgacc atcaccagtt gattgttgtc ataaccggta acacaaagga
     1021 ttgaacgact gtttttaacg caacatactg gccagatgta tcaccccgga cgacctgaaa
     1081 acgcttttcc acccggcatt gtccgccagc gcttatcacc ttccaggtct gttgtagtaa
     1141 atccaccagt tgtgaacatt cttcatcaac tgtgacactg gctcgcgggt gacaaatact
     1201 cgtctggctg agcagcaaca ggcatcgaat tgttgctcct ctatgttgtt cccgcaacca
     1261 gcgtaatacc accggcgagg atggactggc cgtgtgatta cgctcagtaa tacgttcgtg
     1321 cacctgtcgg taaaaggaag tacagaatgc atgaatctgt tgcccgttga tgtttccttc
     1381 tgccgaatag agcgtgaagt gaaatgccaa cagatttggc cagtggtcca ccgcctgctg
     1441 aacaaaacgc cggatgtctc ccggctctga aattaaggct ttggttattt gcacgaaatg
     1501 ttccttttca aaccctgttg aattttgtta agccggtgca gacacacgga aagcgcacat
     1561 caacaccggc acgatacgat acaaacacca gtgactggcg ggacgaaggg aatgacgaat
     1621 cagtcaggaa atgatgacaa acagttggct gaccagagga gtcagcagaa agatgagcgg
     1681 gagttgtcag cgccagcacc aggagcaggg ctgatctcca caataccagc gccgacattt
     1741 tgcacattgc cagcgtaccg gcatcacaac acgtttcatg cagctgctcc tcgtgattcc
     1801 tcaattcgct gctgcatcca ctgctccact tcacttttgt accaacggct gctgcgaccc
     1861 agttttatgg gtttaggaaa atgaccttca ctgatcagct tgtaaaacca tttatcggtc
     1921 ataccggtga aactggtgat gtagttcatg tcgatcaggg gatcgtcggc acgcagcaat
     1981 atactgtcgt ggcctgttat tgaggtcaac atagtcagga tgtcctctgt gagatttatg
     2041 gaggttgggc aaatgctggt gaatctgctg aactgagctt tcagaacagt cctgcagatc
     2101 cgggatagcc gggattaccg gcgaggcaac gggcttccgg atcgcacaca ccacgacgat
     2161 cgcgggcgaa atcactgaac gcccagatca agtctttgag gatgtcttcg cagtccggtg
     2221 aacaactgtc cagcagtaca tccctggtga tctgcttctg gtacggtgct gcttcacccg
     2281 gtgacgcgaa gcccagtacg ttgctccagg catccagaat tagccgatcc agttcagggc
     2341 cgtcgaaata ctcctgtctg acacacagca ccacgtggta ttccggcttt tcaggatgac
     2401 gggtcaggag ccagcaaagg tcgcgtccgg gtttcgcatt catcagcgca ctgacggttt
     2461 cgataaaatc cataccaaac caggtcgttc tgctgttgaa ggtattgtca tattcacacg
     2521 gcagacgaag caggatccgc cgtatccgtg agccggactg ctcacgcagc gcacgctcaa
     2581 gaacgccgat gactttgctc tgataagatg gccagaaggg tgccaggcta tagtcgaaat
     2641 aagggatagc attcataaga tatgctccat tatgcgcagc ccacaatgcg tcctgcccgt
     2701 gaataagctg acgtgttatg gtttgcggat ggggttgatg gaataagtga aaaaccgggg
     2761 caaaacggga agatcaggta actgacaagg aacaaaacac cggatgtggt tcagggtgaa
     2821 tgatctccat tgacttcggt tcacatcatt aagcagggcg gtaagttttt actaacggag
     2881 tgtttccggg ccaggaaact caaggtttaa gcttcacttc tgaagattac gtggacgccc
     2941 tgcggcagga cgctcaggaa cttcactgca gccgtaggca cacagcccgt ctttttgttc
     3001 ttctttcgcc agataatgca gggcaccgcg aatattatcg atgctttcag gatcgttgtg
     3061 gcgcacagga atgttgatgt tgtatgtata atgcggctga tatgtacagc ggtgtgccga
     3121 accgctgtta tgcgtaatag accgccagca ttcagctatc cgctccgcaa agggatatat
     3181 ttttttaacc ctctgacgat cgatccagaa aaccgcatgt gcatgaaaac catgatcagc
     3241 cgtccattca atcacccaga agaagccaac gatattttca aattgttcca cctgctccag
     3301 cagcatacgc aactgcaatt caagccagcg atgatcaggt tgtaagaaat caggcgtatc
     3361 tttcctgtag aagaaatcca tccgtaaagc tcgcaggcag gaataacgtg ccagcatttg
     3421 attgatgtga ttgtcaagta aagcggtaaa aaaccagttt ggtgtgtaag atttaggcat
     3481 gttgatgact cctgaaagtt aatgatgttt taagctctgt gaacgaatta atgctttcgc
     3541 tcataatcag atctaaaacg gtaataactt acagatggta tttatcaggc atgctgtagc
     3601 agaatggata cgacgggtta ttaacgatac gcagcattat gcagaaagaa atggattatc
     3661 tgatggtatt agctaaagag atgagataaa cgaggagcga ataaaataca gaatcaataa
     3721 acatacatca cgaaatactg caaaacacgc gataaaaacc cacgaccaat agttcaaaaa
     3781 acatattacg accaatagtt caaaaaacat attacgacca ataacatcat aaactacaaa
     3841 cttgctctca gtgagtctga aaaaacgtgt acaaggccaa catatacctg taacccaata
     3901 aacttacacg cactcattta atcggttaaa aacaactcat gttcaagata tgaacgttaa
     3961 cgggtacacg gtaccagaat aaaatatacc attgatggat aattttcatt ttgtttaaca
     4021 tacaagataa tcagaaacga acaaatataa tcaggaattc actctgttaa tttcattgtt
     4081 tatcctgcgc tgttaaacat gacggaatca tggataccgt atcaaagcgt aagcattcag
     4141 ttaatcagtg atgacagaga attgatgttc acaagaacag ttaaaacact tcagataaac
     4201 aatcatcgtt aagtcaatac agttcatgat agataagatt aaaatcaatt actgttgcag
     4261 tgaagactga taacagatgg atatcagcaa cagcgtagta gagaataact tatctgagta
     4321 tcatatgagt tacagtaatt atgattgagg gatggagtaa atacgataac aagagtaata
     4381 ctaatggaat gtgtaaagat tgagagtgag tgaagacagg aaataaataa caatagagat
     4441 tgtatgaaaa agggagtgag tataataata gtaataaacc taaattaaaa ttatccttgt
     4501 ttgatgagtc aaaatgagga attatccatt ctggatatga gtctgacaaa acaattctct
     4561 tacagaaaaa tatcaaatcg tttttcttca atcccctatt gaaagctaaa caacatctct
     4621 tatcagtaac agactgataa cctttcgtta ttcagaaaaa gatcgacatc gtgatatacg
     4681 ccagcactca tcacgacaac aatcaaacaa agttatatat aaaaactcac ataatatggt
     4741 taataattag cttcaatcca tgtgaaaaat aacatttcga ttcactttcg agatatcaaa
     4801 aacgaaaaca attcttccac cagtgcacac attcctgcat tcaaccagct ctgacaacag
     4861 gtcattatga aaacaaaatc aaatcacata catggtacat gtactgctac cgttgctacc
     4921 gtagcgattt tttcagagaa attttcgttc acaccccata attggtgaat atttgcacaa
     4981 atgcaggata tgaaaaacgc cttaccatca accttcgaat gaaggcttat gtgttttgtt
     5041 tgcttattgt aaagctgttt caaaataagg tataggctga aaaaggtaac gcaaatgacg
     5101 gaattaatgc cagagggata ttaacgtgat atgttttcgc attatgacta ttcaagggtg
     5161 gaacaatatc aaactgttcg taagacattg caccgcaagg aaggaggtaa cgattgttac
     5221 aatgaacaga aacataaagt gcctgatgcg atcgcatttt gatgatacat aaataattct
     5281 gtgcgattaa ctgattgagt acaggtgtaa gtttttctgc tcttctcaga cgattggggc
     5341 catatctctc aatgtctgtt ttcctgatag catacaaatt attctgcttg attcgggtca
     5401 taatccatgt atacagttca cgggcatcct gttcaaactg gcatcgttca gacataggat
     5461 aaaatattgt gcttgcctcc tccagatacc actccatgag atgcatcgct ttccaaagcg
     5521 ctattgatgt aatttcctca tcgtctttat agcaatagca ggtgaataaa cccgctatcc
     5581 tcagtatatt agaacctgct ttcagaacaa tatctctgat gtgttcccat tccccacctg
     5641 caagcgcttt gctttcaatt ttaatctggt tttcctgcca ctctcccaac gctttcttcg
     5701 aaagttttaa tgtcttcctt tcgcttctca ttccggggga attaatgcta tggcttgaca
     5761 gaaacccgtt aatcttttta tgaaactcca ccagggttgg tattgttacg aactcgcctt
     5821 tcctataatc acgtacccgg gaagagcctc caacctgact gaaaagaaaa cgggaaagaa
     5881 atccgcttcc ccatgccagt acatcatttt tatttatata atccgtaaaa acattcggct
     5941 gagacattaa tgaaaatgtc aggcgtggag taatatgata tttttcgtct gctctcccaa
     6001 aatcaaacgg ttgtccactc catgctttat tcaacagacc cggattattt ttcagatagc
     6061 ttctgaaaaa agttaccgct tcatcagaaa taatccctgc ctccggatgt tcgttgagtc
     6121 cttccgcaag cgctttaagc gaaacatctt catagataaa attcggtcga accggacgtg
     6181 tcggtttatt tctttcgtga ttacgcagtg cctcttcttc ctgttctccc ggatcccccc
     6241 ggttaacagc ctttcttaaa ttggcagcaa gagctttctg ccgggtattc cagatctgta
     6301 gttcagtctt ataatctttg tttctctctt cgtattgttg aatgagtcga tcggcaaatt
     6361 cgtaacaggg ggtcatcacc agtctgttta tcgttgtttt tcccgcgcct ggctctgcga
     6421 ttgccaacag ataaagtgag cagggttccg gcatgttagt gtgaggatga acaacgtcaa
     6481 ccaatgactg acaggagagt gagagcgttg ccagtacagt attaaaaatg agctccagag
     6541 gaagttgggt acttgaatac aagtcgtcaa tgagatacgc aagatcacta ggaaataatc
     6601 tgtaggttac atctgttggg aagtgggggg atgtctgcaa ggcttgcaca taatcgggat
     6661 tcattgtaat ggacctcctt cacatactgt aatcagcaca aagcttattg ttcagttcag
     6721 aaacatacca acattgtgag tatgttcgct gacgccaata ataaaatgac tgatctccat
     6781 tcaccagtcc cggcgcattt atggttctga caaaagtaaa caacatggga gccagaactc
     6841 tgcatcgcag cgacaaacta tggatgcatg aaaaacaggg aacattacgc accagagcgt
     6901 tgagagtctt cctccttgcc caatacgaaa gtcatctatc cacccgtctt caatcttcgg
     6961 gatggttgtt ttatgaacag agtaaaagtt tgaagcaagc tgagaacctc tgtttccacg
     7021 taaaggtccg ctatgaagaa aaatgggagt ttgcatgctc aactaattta cctgtaggtg
     7081 cttgttactg tagttattca acaatcccac aaatatcctg catcagatta cgggctgtct
     7141 ggctaatgcc gccaagctgg aaaaaaccgt gaatgacacc gaggtaacgc tgggcggtac
     7201 actttactcc ttgttgaatc atacgggaaa acagttcctc accttcatcg cgtaatgggt
     7261 catattccgc tgtgatgata tgaacaggtg gtaacccatt gaaatctttt ctgaataatg
     7321 gactggcttc cggatgttct aaatccagac cggaaaaata agcctcataa ccactaagaa
     7381 acgtgtcttc tgtcagcaca taatcacttc cgtttaattt gtaactatcg catgatgctg
     7441 ttgcatccag catagggtaa ataagtatta attttcgtgg taaccactga gcgatgtttt
     7501 tcacccgcag tgctgttatc agtgcaagat gtccccctgc gctatctccc attaacgtta
     7561 ttttgttgcg atcaactttc aacatttctg cgtgttgcca tacaatatta gcggcatgtt
     7621 cagcatcgtc atgtgccgca ggataaatat gttccggcgc taatcgatat tgtacagcta
     7681 tgacacgaca gccgctcagg aaagcaagct gtcgaaactg attatcatgg gttacaaaac
     7741 caccactgac aaaacagcca ccatgataat agacaattgc ggatgacaat ttatcatcgt
     7801 ttaaaggcga aaagatacgc aataaaatat catcaataat cacatcactg atgtttacgc
     7861 gggtttctgt atcgccggcc agagcagtac ttgcaatata tcccgcccga cgttcatcaa
     7921 atgtctgatc acgacaggat ggacgaccac ccgcaacaaa atcatttaca aggtttgcga
     7981 tttcctgttc aagtgccata caaatcaact ccgaccgata atatttaaac aacgctacgg
     8041 gaagtataaa tactctgacc aaaatataac aggtgaatat ccatagcttc aacaggcgta
     8101 atcaataagg gcgtacggag cttgcccgtt attgctgcag gatcgacaga ctggttttcg
     8161 tccttgcgca tgtaacagta caaccacaga gaaaagcatc aatggacact ttatgttgcg
     8221 tcagggcagc gaacgactcc ttttgttgac gacacatatc accttctgta ctggcaatta
     8281 cggcattcag agtctccctg agttttaagc tcctgctctt acggaccagc gacaagcaat
     8341 aaataactat gacaatgaat ctaataacag cataaatatg atactcatcg tgattagtat
     8401 aatcggggca gaattatatt cactgtctga tttattattg ggttaaatat aattaaagtg
     8461 ttctcaaaag tctggctgga ttaccggcat acacgccttt taccagcacg ggctttgtaa
     8521 ccacacttcc agcaccaatc actgctccac tgcaaatttt tgttgttaaa atagtcgctc
     8581 cgctacccac tgttacagtg ttacctaata cgatacgtat ccagctttct gcagaggcat
     8641 cgggaccacc acacttaaaa agatcattag caaaagttac gccatggccg atgaaacagc
     8701 catcacctaa aatgacgttc tcacagatga aactgtggga ctgaattcgg ctccggttgc
     8761 caatgatgca tcccttctgt atttccacaa agggaccaat aaaaacatca tcccccagtt
     8821 cacattcata gaggtttact ggcataacga ctttcacatt ttcaccaaat ttcacatcac
     8881 gaatgccgct gtcaataacg ttcatatccg ttgtatcctc cctcaggatg attcttatca
     8941 gaacatttca cctgtcagaa tattcccggt aatgtgatac aagatccagt ctcaccgggt
     9001 accacgtgtt attgcccgta tccttttgcc cgttacgagt ctacaactgg ttttgtattt
     9061 tgcaatccag gcctgttaca ccagagcatc ctctcacttc cccctcactg gtcagtccgg
     9121 gattcaacat catcctccag cactaaccct ataccagtcc tgaaccttcc ctcgccagca
     9181 gcctactgtt caccacaatc ctcttccgtg aatggcagta agtgagctgc accaaaataa
     9241 atcggtactt ccgatgacag acagtggaag tgccaggaga aatatgaaca tgagaatgga
     9301 agcagataaa aataccgttg catgacttca ggacgggtta tcagaatacg agctaagggc
     9361 gtcagccaac gcccttaccg tggtatacac tcaggcaccg gataccattt taccgggttc
     9421 acctgccatc cagtagacac ctcaccccac gactgcaacc ataattacgt tgacctgaac
     9481 cgtcatgcag cttcgtttca tttttcacga gataacgggc tctctccagg gctttctgca
     9541 ttgcttccac atcaccacgc ttggcccaga gcacagcgga tccacccggc cttttatcag
     9601 aatccgtccg ggtatcagaa catccactat gcaaaggcgt gtcatcggaa gaaggggaag
     9661 catcagccgc tgctggttta cgggaaggcg tccacctgga aaaatggacc aggccatcgc
     9721 cttgccaggg ctcaggatgc agagcgctgc accatgcctg ttggatcatc gtcgccagcg
     9781 aagacggttc gctgaaatcc ccgagcgagc accagatatc tttgttgagc agtaatatca
     9841 cgtgataatg tttcctgcct ttttccttgc caaactcccg acaccagaaa taacgcaggg
     9901 tggttgcatg cacacgctga ttggcacatc tttttttccg ctggtaagcc tgaattttgg
     9961 ctttcaggga tgcaaaaaaa cgagaaatca ccgctgaatc cggatcggga tccatgatgg
    10021 tgacaggcat atactcaggg aaacgtaaat ccacccggat taacgagacg gcacggtgct
    10081 gcgccggggc actgtcgaga aggctgtcaa aacgggataa ccagtaagca ttgtgtattg
    10141 attgattcat gatgagagat ctccgttgat ttggattgac ttcacatcat tcatcaacgc
    10201 ggtgaaactt tacggcatcc cgccaatgag caacgccaat agctcctgat taaaacggaa
    10261 tgaatcgtcc cgtaacgaaa agataatcct gcacacgaca gcaaaacgta aaacaacagc
    10321 atgaatgagc taaatgttta aagaaaaaat gtcttttcac attatttttc agcaagtaat
    10381 cataccagca tccctaacat acgaaatcac atagtgatcg atcgctgatt cgcttaacaa
    10441 ctgatcgaat acaccggaat ttacactaac atcctgatgg gtaacggaag actaaaaaac
    10501 ctgtgttcta ttgattgcac ggaatgcctg tgcactggcc tgaccagtaa gcacaattct
    10561 acgagtagta agatatctac gccagaacca tgcggcagta gccaccatat gttgccaggg
    10621 tgaaaaataa gctcagagct ataggggagt aattcccgtt aatgtcatcg ctgtacctca
    10681 atcagttcac aaactggctg aaacgttaag ctgaagcctt tattcttcag atgtattttc
    10741 cggtttacct gtaaattatc ttctgatatt tatcacatta gtagttgtgc tgattatcta
    10801 catacttcaa ggtctgaaat atgaagctaa cagaagaaag tctgaatcta gtcattgaca
    10861 tcctgaatga tttgatctgt gaaggaaaaa tgctgaacga tgtggagcgc agatctctgc
    10921 tcaatgcagc tatgacaatt acagcggtaa aagaacgttc tgtcgtatct gtatcatccc
    10981 gaaaagagca aagtaagaag gagaagaagg aaagagagat cgatcctcgt ttcccaaatg
    11041 ctggtgtggc atggaatgag gcagacgaga gtttattgcg agatgttctg gaggatgtac
    11101 cggacgatga aattggtaaa caccttttct ggctggcaga gaaactaggg cgaaccccgt
    11161 acagtgtagc ctgtaaagtc agacaaatta agaaattacc tgcagaatgg aaggatcaat
    11221 ttcgtaaaat ctctgacagg atccgtagtt ctggaatgtc agtcagtgaa tatctacaac
    11281 gccagggact gttaagccca gagtcagggg aagaaaacaa tcctgtcagt cataacaaca
    11341 atgtagctta atcctgaaga agatgaacag tttatactct ttaaactgtt cccttaattt
    11401 tagaaaaaca gtttgtgtaa gtgcatgctc caacattctg aaggagttat ttcttacaat
    11461 acatcataat cattggcaga tcctcatctg aatcaaaaca ccacaaccaa tcatcacact
    11521 ttttctgaaa agagccttat gatgaagata atcaccttac gctgagaaaa aacaccaggg
    11581 ccagttaatc accttatcaa tctcgccgtt acaaaatcat ccataagcca tttaacagtg
    11641 ttatcgcaaa ctcacagtaa atcgcatcag gttacctcaa tcggatttga gttaattcac
    11701 tgggctttac gtaaagatac ttttctcagg ttaacgacta aaacacgaaa actcagaaaa
    11761 ggttaaacac aggttcagta aaatcgaata tcatcttgat tgcttaaaca ggacagaggg
    11821 ttttatagtg aggttaactg attttagcga tcgtaaaaaa aggactaaca aatttactaa
    11881 actggatgtg tatcagacaa agcaatgtgt atagcaatgc ttaatgaaaa tacttgtatg
    11941 attgtctatg aaagtacgtg tatatagtat cataccgaca ttctcaactc ttccaaacat
    12001 ccatgcagac tgaatgttgg tttcagtttt tcacggccag catggggatt tttgttctga
    12061 aaacggagca gatttactct ggaattattt tccctgtcgg gatatttttc acctgaaaac
    12121 tttctacaac acaacggctg tataacatca ttctggcata ttcattcagg tgagtcggac
    12181 aagcgataat cctgttcttt accgaacgaa caggcagcga taaaaaactg attcagacga
    12241 catttctgtc tgacaaacat tcacatgcag aagaccggga catttattct tcccgaaagg
    12301 aagtgatacc ttttcttatc atggtagctg cattacctga tatctttcag gcgaatcccc
    12361 tccctgtaaa tctgatcgtc agataaaaga gatgaacatc aggcagattt ccgtcaataa
    12421 cgataagaga tgaccatcgt aaacatatct tccagtaacg aactggcacc tgcatattac
    12481 cgtttattct catacatcat tgaaatattg tcagttataa aggacacaca tcaggaaatt
    12541 atcaccatgt gacaacgtca cagatggtgc tgatatataa aaattaacaa aatcgcgcca
    12601 aaaaaccgcg ccgatattgc gcatctgtaa tccatcattt cctttttgaa taaccttccg
    12661 tggttacccg acatcctgat aatgacgccc atcgtacacg cttttctttg ctcagacatc
    12721 gcatactttt atcctgctca ataccgtctt cctgccagtc ttcccactct ctgacactca
    12781 cagaacagca acgtcctccg gacatactag cagggcagaa cacaatccac aaca
//
LOCUS       DAZKJR010000138        12812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKJR010000138 DAZKJR010000000
VERSION     DAZKJR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.139"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(130..849)
                     /gene="dcuR"
                     /locus_tag="ACHOWS_004117"
     CDS             complement(130..849)
                     /gene="dcuR"
                     /locus_tag="ACHOWS_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFQ0049612.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(846..2477)
                     /locus_tag="ACHOWS_004118"
     CDS             complement(846..2477)
                     /locus_tag="ACHOWS_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0049613.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2657..2887
                     /gene="yjdI"
                     /locus_tag="ACHOWS_004119"
     CDS             2657..2887
                     /gene="yjdI"
                     /locus_tag="ACHOWS_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFQ0049614.1"
                     /translation="MDQALLDRGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2899..3171
                     /locus_tag="ACHOWS_004120"
     CDS             2899..3171
                     /locus_tag="ACHOWS_004120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0049615.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3398..3694
                     /gene="ghoS"
                     /locus_tag="ACHOWS_004121"
     CDS             3398..3694
                     /gene="ghoS"
                     /locus_tag="ACHOWS_004121"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFQ0049616.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3722..3895
                     /gene="ghoT"
                     /locus_tag="ACHOWS_004122"
     CDS             3722..3895
                     /gene="ghoT"
                     /locus_tag="ACHOWS_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFQ0049617.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4014..5531)
                     /gene="lysS"
                     /locus_tag="ACHOWS_004123"
     CDS             complement(4014..5531)
                     /gene="lysS"
                     /locus_tag="ACHOWS_004123"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0049618.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(5768..7225)
                     /gene="dtpC"
                     /locus_tag="ACHOWS_004124"
     CDS             complement(5768..7225)
                     /gene="dtpC"
                     /locus_tag="ACHOWS_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFQ0049619.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7284..9431)
                     /gene="cadA"
                     /locus_tag="ACHOWS_004125"
     CDS             complement(7284..9431)
                     /gene="cadA"
                     /locus_tag="ACHOWS_004125"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFQ0049620.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSSWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYEDMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9511..10845)
                     /gene="cadB"
                     /locus_tag="ACHOWS_004126"
     CDS             complement(9511..10845)
                     /gene="cadB"
                     /locus_tag="ACHOWS_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFQ0049621.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTAGTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAGSGAPFAISASTILGNWAAPMVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11211..12749)
                     /gene="cadC"
                     /locus_tag="ACHOWS_004127"
     CDS             complement(11211..12749)
                     /gene="cadC"
                     /locus_tag="ACHOWS_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFQ0049622.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         3543 a         2971 c         2968 g         3330 t
ORIGIN      
        1 atttttaacg atgatggagg gataattata tttgatctgg cacaagtttt actgatgaag
       61 aatgtaactt gtgccaggaa tattttgcat tacagtaata attattactt acagataacc
      121 agcgatccgt tattggcaat attgtttcag cagtgagtag tgttctgcct gaatacggta
      181 acggtaaacc ggacgccctg tgacgccata atggatactg gtgaacaaga tgtggcagtt
      241 gaccagccag atgaggtatt tacggcagga aacacgcgag atgttaacct cgttggccag
      301 ctcgtcggtt gaaaactcat agtcctgatg cgcgtcaatc cactggcaca gcgtgcgtaa
      361 cgtctgcggc gtcaagcctt ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc
      421 gtggattagc tgatcaagct cggcctggtc ataatactga tgtttttcca gcgccatttt
      481 cttttgccgc cagccggtga gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag
      541 gtaatccacg acaccgtaat gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga
      601 gatgacaatc acatcacttt tgcaacgcgc gttatgcagg acaggcagta aatcgagccc
      661 gttctctttt tgcatataga tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa
      721 gataatctct ttggctttct ccagcgtcga agccgttcca cagcattgaa agcctgggat
      781 ttgtgccacg tatcggcgat tcagctccgc gaccattgcg tcgtcatcga taattaatac
      841 attgatcatc tgttcgacct ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa
      901 atcccgggtt ccgattccac ggcgatgctg ccgccgagat tttctacctg ttgtttgaca
      961 agtgctaaac cgacgcctcg ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga
     1021 tcgattttgt cgggtgcgat ccccggtcca tcatcgttaa cttcacagtg cagccagccg
     1081 tgacggtagt gcaatgttac gctaatttcg ccaccgggtt ccggtcctaa tgcctccagc
     1141 gcgttttcta tcagatttcc cagcgtggta atcagcgtcg cgacctggtc ttcactgccg
     1201 ctgtctggca actggctttc actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcgg
     1261 ttaatcttac tgattaaaaa accagcgata accggagatt tgatcttacc cagcagagag
     1321 ccaatctctt cttgatagtt attggctgtt ttgagaatgt aatcttccaa ctgcttataa
     1381 ctcttcagat gcaataatcc gagaatcacg tgcaatttat tcataaattc gtgggatcgt
     1441 tcacgaagtg cgtcagcata gttgaccaga ccatcgagtc gctgcatcag tttacgtact
     1501 tcagttttgt ccctgaaggt tgaaatggca ccgatgataa cgccattact gcgcaccgga
     1561 acggtgttga tcagtaatag ccggtcttta atcgtaatct cttcgtcgcg ccgcggggta
     1621 ccgtcgcgta acacttccga gacatctacc acctgtgacc acgagtggct tagcgtcgac
     1681 agcttctcat cgtcctgcga cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc
     1741 agcgtgactt caccgcgatc gtccacggca acgacgcctt ctttgatgga ctgcaacata
     1801 gcctggcgtt gctcaaacag cgtggagatt tcgtagggtt ccaggccgaa aaggattttt
     1861 ttcagtacct taaccagaat gcaggtgcca atcagcccga ccagcatgcc aaataatacc
     1921 gaccagatga tgctccagcg actgtcattg atctgttggg tcacgcgact taactcaagg
     1981 ccgatcgcca ccacgccaat ttgtttatga ttttcatcgt agatgggggt aaatacgcgt
     2041 aaagcctgcg ccagaaaacc gcgattgata gcgacatttt cttcgccatt cagcgcttta
     2101 aggatgtcat cacctttaaa tggctgacca atacgctggg cttcaggatg cgagtagcga
     2161 agactttgca tatcggtaac gacaataaac agcagatcgt tgcgtttgcg cacggcttcc
     2221 gcgatggcct ggattccact ctcctgtggt tttttctgca agccctgacg gatttccggc
     2281 gagtcggcga gggtacgcgc tactgccagt gccttgttag ctagcccgtc tcgcgtcata
     2341 tcactgattt gcgagaagta aatcagatgc accaccaata gcaccgagaa cagtaccgcg
     2401 ctgaccatta agatcactgt ggtactcaat ttcatcggac gtttgcgtaa catgcggtag
     2461 ggcaatgaat gtctcatcag cttccttgtg tgacaaattt cttaagcatt atctctgatg
     2521 aggcgggtaa ttcaaaggga gtaagaatga ttggcatata ggggaagaga ctctggcaac
     2581 ggaaactgcc agtgctgtat gaagattcca gggctatgct tatagtgaaa accatactga
     2641 tgagagaggg aaggtcatgg atcaggcgct actggacagg ggttatcgct gttataccgg
     2701 cgaaaagatc gatgtctatt tcaacactgc gatatgtcag cattctggca attgcgtacg
     2761 tggcaacggc aagttattta atctcaaacg aaagccgtgg atcatgccgg atgaagtcga
     2821 cgtcgccact gtggttaaag tgattgatac gtgcccgagc ggcgcgctga aataccgtca
     2881 taaataagcg agggtaaaat ggaaatacgc gaaggccaca ataaatttta cattaatgac
     2941 gaacagggca agcaaatcgc tgaaattgtc tttgtgccga ccggagagaa tttagcgatt
     3001 atcgaacata ccgatgtcga tgaaagcctg aaagggcaag ggattggtaa acagctggtt
     3061 gcgaaagtcg tggaaaaaat gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt
     3121 gcgaaacacg aatttgataa aacgcgggag tatgatgata ttcgcagttg atgggagagt
     3181 acagagtcac gatatttttc atgctctccg cggtgtgatg caggagagca tctgaagggt
     3241 agggggatgc acaaagaatg ggcagagagc gcgttttttt gtcccaagtc atccccttta
     3301 ctgagcaaaa aaaagaatat ctcctatatg agaatcatca atcggggtta ataagttttg
     3361 cgtccccaga gcgtttaata ttgataggag tcatattatg gaaggtaaaa acaagttcaa
     3421 tacttatgtt gtttcttttg attatccatc atcttattcc tcagtgttct taaggttaag
     3481 atcattgatg tatgatatga gtttctcctc tatcgtggct gatgaatatg ggataccacg
     3541 acaactgaat gaaaactcct tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga
     3601 tttaatcagg cttaaatgct tagacttacc ggatattgac tttgacctca acattatgac
     3661 agttgatgac tatttccgtc agttttacaa gtagccagga aggataagaa gaacataaaa
     3721 aatggcacta ttctctaaaa tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt
     3781 cattatctgg tttatctcac atgagaaaac acatattcgt ttacttagtg catttctggt
     3841 cggaataacc tggccaatga gtctgcctgt ggcattactt ttttctctct tttaggagct
     3901 gtagttgagt ggacccgctg gttatcttga aacgaagcgt ctctcctcaa aactggattt
     3961 gcggaatgac gaacgaagca gtcgcctgac tgcttcgttc attaaagtga aatttatttc
     4021 agtgggcgca tcgccgggaa gagaataacg tcgcgaatag tatgactgtt tgtaaacagc
     4081 ataatcattc ggtcgatacc aatacccaga ccagcggttg gcggcagacc atattccagc
     4141 gcagttacgt aatcttcgtc atagaacatg gcttcgtcgt cacctgcagc tttagcgtta
     4201 acctgttcct ggaaacgttc agcctgatct tctgcgtcgt ttaattcgct aaaaccatta
     4261 ccaatttcac gaccaccgat gaagaattca aaacggtcgg tgatttccgg gttaacatca
     4321 ttacggcgcg ccagcggtga cacttctgcc ggatattccg tgataaaggt tggctgaatc
     4381 agatgtgctt ctgccacttc atcaaagatc tccgtgacaa tgcgtcccaa cccccagctt
     4441 ttctctaccg taattccgat agattcagct aatgctttag cagcatcaaa attatccaga
     4501 tccgccatat cggtttctgg acgatatttt ttgattgctt cgcgcatggt gagtttttca
     4561 aacggtttgc cgaaatcaaa cacatgctcg ccataagtga ctttagtggt acccagtacc
     4621 tcttgtgcca gagtgcggaa cagtgattcc gtcagttcaa tcaaatcgtg gtaatccgca
     4681 tacgccatgt agagttccat catcgtgaac tcaggattat gacgaacaga aataccttca
     4741 ttacggaagt tacggttgat ttcgaatacc cgttcaaaac cacctacaac cagacgtttc
     4801 agatatagct ccggcgcgat acgcaggtac atatctaaat ccagagcgtt atgatgggta
     4861 ataaacgggc gagcagatgc tccacctgga attacctgca tcatcggggt ttctacttcc
     4921 ataaagccgc gagcgaccat gaactgacgg atagcggcca gaatttttga acggacaaca
     4981 aacgtttgac gggatttatc gttagcgatg aggtccagat aacgttgacg ataacggact
     5041 tcctgatcct gcagaccatg gaatttatct ggtaaaggac gtagtgcttt agtcagcagg
     5101 cgcagctcag tacagtgaat ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg
     5161 gcaccgataa tgtcacccag atcccatttt ttaaactgat cgttataaac accttctggc
     5221 aggctatctc ttgcaacgta cagttgaata cggccaccga catcctgcaa cgttacaaag
     5281 gaggctttcc ccataatacg acgggtcatc attcggccag caaccgagac ttcaatgttt
     5341 aaggattcca gttcctggtt atccttcgca tcaaactctt cgtgcaactg gtcagaggta
     5401 tggtcgcggc gaaaatcatt gggaaacgcc acaccttgct gacgtagtgc cgccagtttt
     5461 tcgcggcgat ttctcagttc atcgttaaaa tcaatagcct cattggctcc ccgtgtttct
     5521 cgttcagaca ttttggttcc tctaaatcca gctttcaatt tagcgtagat aaagagacag
     5581 atcggtacac tgtattatct gcctcgacta ttaataactc agtttatctt atcgaaacta
     5641 acgcttacct caagttgata tcactaataa ataaccataa aaatggttta aatgacttat
     5701 taatctcgat ttgctagtgt gataaactga aagttaattt attcataacc aattgttttt
     5761 acgacagtta atcgttgctc tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg
     5821 tagcaaaggc caataccacg atgatcgcga cacaagccaa cgtccactct cccatctgag
     5881 aaaagaatcg ctggtaagcg gcaattgcca taccgctaat ttgcgactct gtcgtctgct
     5941 gtgccacgac gcctgccagc cagttggcga ccgcgcctgt tgccagcata taaataccgg
     6001 ttaatacgcc agacattttc agacgcgtga tttgcgcaat cgccaccggg tcaataaaga
     6061 gttcagcaaa gcccattagc gccagcccgg atatcatcac gcccattgac gcttgaccgc
     6121 cagccgctgc atgtcgggca tcaaatgcca gcaacataaa gccacaagcc atcagcagta
     6181 ggccaaaggc aaacttcagc cagacgcgca atgctgaatt gccgcggctt tctggcgacg
     6241 ccagccaggc cagaacaacc ccagcgagca tcaccgcaat agcattcacc gactggaata
     6301 gtgctgtagg tacttcaata ttgaatgcct gacgattcac aaagcgatcg ataaacaagc
     6361 tgatggtact gccgccctgt tgtgccagta cccagaacaa cgtcccgaca aacatcaaca
     6421 atacaatttg ccaaagagca cggcggtgtt cggggaattt gatcatcatg cgggcaatga
     6481 tttgtgctgc aatgaggcaa acgatcgcca gcaaatatcc ggaccagtcg ttctccagca
     6541 gcagggtaaa aaatactggg gctaaacaga gcatcaccac taaccagctc catactggta
     6601 aggcaaattt gacgctagtg agcgcttttt tatccatact acgcgtggac tggaaatgac
     6661 gatgaccact taagaaaatc aacaaaccga taaacatgcc gcctcccgca agggcaaagc
     6721 caacatgcca tccataccac tgagcagcca ggccgcaggc gatgggcgcg gcgatagaac
     6781 cgatattgcc cgcagcatat agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt
     6841 cgtagagctc gccaagcaaa cagctgatgt ttgatttgaa taaaccgtag ccacaaataa
     6901 tgattgccag cgccagatac aggctaaagg ttgaatttgt atcaatgccc agcaccacat
     6961 ggccaagggt cattaacagc gcgccggcaa tcactgcagt gcggttgccg agcaggcggt
     7021 cggcaagcca gccgccgaga ataggggtaa cgtaaaccag agaagcatat gcgctgaaca
     7081 ggctgatggc atggttatca tcaaaaccaa gctgatgggt gagatagaga atgagtaagg
     7141 cacgcatgcc gtaaaaactg aagtactccc agatttggat cgccacgata tagtatatcg
     7201 cgcgcggctg tgagggtgtt ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa
     7261 gccacttccc ttgtacgagc taattatttt ttgctttctt ctttcaatac cttaacggta
     7321 tagcggccat cagcctgacg gtatgcaccg tgaatatcgg tttcaaagcc cggatagtga
     7381 gcgccgattt cacacagcat ctgcaggaac tccagaaccg gacggctttc ttcggtgatc
     7441 atttcacccg gcattaccag aggaactccc ggcggatacg gaaggatcat attggcgtta
     7501 atacgaccta ccatttcgtc gaggtaaact tcttcggtca taccgtgcag ctctttctgg
     7561 aacgccgcat acggagtcat aaccattgtc ggcagaactt caaatgcgcg atacatcaga
     7621 tccggcagat tgtggtgaac aatcagtttg tggatattct gagccagttc ctgaatacgc
     7681 atatcttcat agaattcagg atcttcacga tatagagacg gcagcatgtt tttcacacgc
     7741 aggttcaggt cgaacgcacg tttgaagtca gtcagagcac gcagcaggct caatgctttg
     7801 gtcttatcaa taccgatgct gaacaggaac agcaggttat acgggccggt tttctcaaca
     7861 acgatgccat gttcgtcgag gtatttcgcc acgatgctgg ccggaatacc aaagtcgctc
     7921 atggtgccgt ctttttccat ccccggggtc agcagggtga ctttgatcgg gtcaagatac
     7981 atgtgctcat tgtcgatgtt cttgaagccg tgccaggagc tgtcagaacg cagcggccag
     8041 cattcagtgg tatcgatatg atccggctgc catacatcaa agaaccagcc atcagattcc
     8101 gttctcagac gtttgatctc tttacggaac ttgatcgcac gttcaatgga accgttgatc
     8161 agacgcttac cagcattacc tttcatcatc gccgcagcgg tttcagtgga cgccacgata
     8221 ccgtagtgcg gagaggtggt ggtgtgcatc atgtaggctt cgttaaaggt ttcttcgttt
     8281 acgtcacctt taacgtggat catggaagcc tgagagaacg ccgccagcag tttgtgagtg
     8341 gactgggttt cgtaaatcac tttcccttct acacggccac cgctcatacc gcatttacct
     8401 tcgtaaatcg gcgagaagtt ggtgtaaggc acccacgcgg agtcaaagtg gatggatttc
     8461 acatccagcg ttttcttgat gaagtcggtg ttgtacagca gaccatcata ggtagagttg
     8521 gtaattacgg catgtaccgg ccaggttgcg tttggtgttt ctttaacgcg cttagcaatg
     8581 gttgcgtgct ggaattcact ctgtgggata ccaccaagaa taccgtaagc gttacgggtc
     8641 gggcggaaat agattggggt aacatcgctc atcatcatta ggtgggttag cgatttgtgg
     8701 cagttacggt caatcagaat ggtgctgcct gccggagcag agtacatacc gacaattttg
     8761 ttcgcagtgg aagtaccgtt ggtcaccatg tagctgcggt ctgcgttaaa gacgcgagcg
     8821 atatactgtt ccgcttcttt gtgtggaccg ctgtgatcca gcagagaacc caattcagat
     8881 actgaaatgg aaatatcaga tttcatggtg ttcggaccaa agaaatcata gaacaggcta
     8941 cctaccgggc ttttctggaa tgcagtaccg cccatgtgac caggagtaca gaaagtatat
     9001 ttaccttcac gaacatattt aaacagtgct ttggtcagcg gaggcagaat agtgttgata
     9061 tattcgtcag tggtctgctt gatcttgtta gcaatatcat cagcagcacc cagcgcatat
     9121 tcaaagaagc taatctgtaa acgcagatcg ttcaggctta catcgagtgt ggaatacgta
     9181 ttagcgaacg cgtacaacgg caggttctcg ttcattttgc taatttcttc gcacagctcg
     9241 agattatatt tatcccagtc aaaaataaca ccgcacagac gcgcattgtt ttcgatcagt
     9301 tttaataagt cgtcacggtc attcgggtaa acaatctgga agttcagacg ttcaagcgcg
     9361 cgatgaagtt cacggatggg ttcttcttta aaataaacac ccatgtgatt caatattgca
     9421 ataacgttca tagtcatatc tccaggtaaa aaaggcccct cccaacacat gggacaaaat
     9481 gaaaggagga gcctcggaaa atacttttaa ttaatgtgcg ttagatgctg tgtggttatc
     9541 cattgagtgg ctctggcgct cgtgcatttt gcgagcgtag aacatcagga taatcaggct
     9601 gacgatgaag gtaccagcca gctcgaagga gcttgcgccc atcagagcga tgaagcagaa
     9661 cacgcaaccc agtacagagc agatcaggct gacaaagttg cggatgttaa cgccttcaaa
     9721 acgaatcagg tcaacgcaag agtagaagta cggcagcata gtcagcagta ctgcgatacc
     9781 ggtcagttca ccgaacaggt cagatgcttt accaccggca gagttcatca gagtgatgag
     9841 gatcatcagg gcagtcattt tcactgcagc cagcagcaga ccttttttcg gaataccgtt
     9901 gctgtcgact tcaccataaa ctttcgggaa gttaccgtcg ttagcggcac gcacacctgc
     9961 ctggccgacc agcatcatcc aggagcccag agaagtcagg cacgcaaaag cggtgaatgc
    10021 agaaaccatc ggcgcagccc agttacccag gatagtagaa gcactgattg caaacggagc
    10081 accggagcct gccattacag aagacggata cataccggaa agaacctgag tcgcagcaat
    10141 gtaaacaata cctgctaaac cagtccccag catggttgcc agtggaacag tacgtttcgg
    10201 gtttttaacc ataccagtac ttacagctgc ggattccaca cccacgaagg cccacaggca
    10261 gagcagaata cttttaatga ttgcatgacc atcagtagta cctgcggtat tccagttagc
    10321 tgcataagtt gcagcatcaa accaatgcca gccaacaata gcagtcataa ccacaggaat
    10381 aagaaccagt accaggccaa tagtggttaa acggcttacc caggtaccgc cgagcatatt
    10441 tacaaaggta aatacccaga cgatagcaat acaggcgata cccgccggaa caggatcatt
    10501 taatactggg aagaaggtgg aaagataaga tacagcggta ataccaattg ccaggttacc
    10561 aatccagtta gcatggtaat aaagaacacc tgtctgaaaa ccaaatgcag gagaaatttc
    10621 tccggcatac gcaattgggc caccttgttg cgggtttttt gttgccagtc gggcatatac
    10681 atatgccagc gacattgcac caataataga gataatccaa ccccagatag caataccacc
    10741 gatacttgct aggttcgcag gtaataatgc aataccgctc cccatcatat taccggcaac
    10801 aacaccggta caggcaaata gcccgatctt cttggcagaa ctcatgctct tctcctaatt
    10861 tcatttttga atttggagtc cgggtcatga tgtataacta tttcctgacc agaccaaact
    10921 ggcgctaaga ttactcacga aaaaaggatt aatcctaaag attaggtgaa ataacacaaa
    10981 agtttctgta agtgagaact tgaggttttt tattaacaca tcaggatcgc aagttgatat
    11041 catgaaaaga taaacattca atgtttacaa tgtgttatgt gacattttct ggttaaattt
    11101 atgtaataaa aattatgcgg caaataaatt gccgcaacat attataccaa caggaacata
    11161 caaaaactca acaacaaata ttcccgagca tgaatcaacc cgggagttac ttattctgaa
    11221 gcgagaaatt tgtcgagata aggtacaaca taaggaacag aagtctggaa tataccattt
    11281 tcaatccagt aaagggtgtt tgcccctggg cgtaaattaa aggcggtgag atatgcatca
    11341 gctgcttccc ggttcatccc cttcatttca taaaccttgc caagcaacac ataatttagc
    11401 caggacattt caagatcaat gccagtattt atcgcctgat aagactcatc tgttttacct
    11461 tttaccaggg cactgaccgc ttttatttga tatataatgg acaggttgtt caattccggc
    11521 agtgtaacaa tgttatctat ttctgtgtta agtgctgcta attgtttttc gtctaaagga
    11581 tgttgagaat ggcgcacgat atcaactaat gctttttctg ctctcgcgta ggtaaattct
    11641 ggggatgatt gaacaatctc acctaataat tcactggcac gatccaatga tttatcatca
    11701 ccatgcagta aataatcatg tgcctgataa aaattagtta ataacgcacc acgatgcggc
    11761 aaaatttgct ggagcatctc ctgcattcgt tgtggccacg gttggtttaa cgcttttgat
    11821 aaactctcca gtaaatcatt ttgaatcgcc agctgattac cgttagtgat gacataacgt
    11881 ttatccagca tggttgaacc atctgcattg tctaccaatt ttaccgacat aaagcattgt
    11941 tgagcacggt attggcgctg attaacaaac gcaatagata atgttttacc ggaactgctc
    12001 ggttcatcaa tgttgtagtt gattttgtca tgcaccataa aggtggagaa ggtgttaagt
    12061 gatgtcgcca ccaaatcacc cacgcctatc gcgtaagaga gctgatacgg ggaactccag
    12121 ctgttacaac ttttatttac catattaatg tcaatatcgc gtggattgag caaaatacgc
    12181 gatttgctca tcggaagacg tgtatcaaga cttgaaaacg ctaccagtgc tacacagata
    12241 cctaacgaca acaggaaaaa aaaccatacc caaaaggtag tgaatcgttt gcttttaact
    12301 ggggattgtt caggtggcgt tgcggtgttt tgaatgttaa gactgtggga gggagaatct
    12361 gtggcaggaa ccgcctctgg tataggggga ggcgaagata gcattatttc ctctccctct
    12421 tcttcgctgt accagataac cggcaccatt aatttatagc cgcgctttgg tacagtagcg
    12481 atatagacag gactatcttc atcattatct tttaatgact tacgtagttc tgagatactc
    12541 tgcgtcacaa cgtgattggt gacaatactt ctcttccaga cattatcgat aagttcatcc
    12601 ctgctaagta cttcgccact gtgttgagca aagaaaacca gaaggtcgat taatctcggc
    12661 tcaagggtaa gttgacgccc attgcggcta atttggttta tggacggagt aacaagccat
    12721 tcgccaacgc gaactacagg ttgttgcata atagaaactc attcgaaaag ggaatggtgc
    12781 aatgataatt ggcataacct atttttacca tc
//
LOCUS       DAZKJR010000139        12777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKJR010000139 DAZKJR010000000
VERSION     DAZKJR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.140"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..927)
                     /gene="yhaJ"
                     /locus_tag="ACHOWS_004128"
     CDS             complement(31..927)
                     /gene="yhaJ"
                     /locus_tag="ACHOWS_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFQ0049623.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1032..1733
                     /locus_tag="ACHOWS_004129"
     CDS             1032..1733
                     /locus_tag="ACHOWS_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFQ0049624.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1756..1920
                     /gene="yhaL"
                     /locus_tag="ACHOWS_004130"
     CDS             1756..1920
                     /gene="yhaL"
                     /locus_tag="ACHOWS_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFQ0049625.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2052..3362)
                     /gene="cyuA"
                     /locus_tag="ACHOWS_004131"
     CDS             complement(2052..3362)
                     /gene="cyuA"
                     /locus_tag="ACHOWS_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFQ0049626.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYEIISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3390..4721)
                     /locus_tag="ACHOWS_004132"
     CDS             complement(3390..4721)
                     /locus_tag="ACHOWS_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0049627.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGVLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4996..6360)
                     /gene="tdcG"
                     /locus_tag="ACHOWS_004133"
     CDS             complement(4996..6360)
                     /gene="tdcG"
                     /locus_tag="ACHOWS_004133"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFQ0049628.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6432..6821)
                     /locus_tag="ACHOWS_004134"
     CDS             complement(6432..6821)
                     /locus_tag="ACHOWS_004134"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFQ0049629.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6835..9129)
                     /gene="tdcE"
                     /locus_tag="ACHOWS_004135"
     CDS             complement(6835..9129)
                     /gene="tdcE"
                     /locus_tag="ACHOWS_004135"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFQ0049630.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9163..10371)
                     /gene="tdcD"
                     /locus_tag="ACHOWS_004136"
     CDS             complement(9163..10371)
                     /gene="tdcD"
                     /locus_tag="ACHOWS_004136"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFQ0049631.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10397..11728)
                     /gene="tdcC"
                     /locus_tag="ACHOWS_004137"
     CDS             complement(10397..11728)
                     /gene="tdcC"
                     /locus_tag="ACHOWS_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFQ0049632.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11750..12739)
                     /gene="tdcB"
                     /locus_tag="ACHOWS_004138"
     CDS             complement(11750..12739)
                     /gene="tdcB"
                     /locus_tag="ACHOWS_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFQ0049633.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3255 a         3502 c         3045 g         2975 t
ORIGIN      
        1 ttcgcccctt tttattaaca ataacaaata ttattttccg ctaaaaagtt tgggaatttc
       61 ccgcagacac caggatttcg cctcccccat actgtcacga cgccaggcca taataatatc
      121 gatctcgctg gtcgattccg ggctgacgac acgcaaccgc ccttccgcga tgtctttttc
      181 gaccatcgga tacggcatcg tcgccacgcc aagccccgcc agtaacgcct gacgtttatc
      241 ttcaatcgtg ctcaccgtta aacgcggctg tttgtccagt agctgtacgg tcaataccgg
      301 gcgctcacga gcggtatccg ccaccgcaat tccacgatat ttcacgcgcg tcacttcaga
      361 taacggttcc ggctcctgat gaatcgggtg atcaggcgcg gcaacgtaga cgttcattaa
      421 cgtatagagc ttgcgcgagt tgatctccga cgaggagcga aaatgcatat ccggcgcgat
      481 aacaatatcc gcccgcccct gctccagccg ttcccatgcc cccgccagca cttcggtgat
      541 gattgccagt tgggtattcg cttttgccgc cagtttgtca attaacggga aaaaggcagg
      601 tgtcggcacc agcgcttcgg tcacaatggt gagatgcgtt tcccaaccgc gcgcgagagc
      661 ttccgcatcg gtagtcagtt tatctgcggc ttccagcaaa acgcgtcctc gctccagcaa
      721 catcctcccg acattggtga atttggtgcg atggcccgag cggtcaaaca gcaccacatc
      781 cagctcttct tccagttttt gcatggtgta gctaagtgcg gaaggcacgc gtcccagctc
      841 atccgccgcc gccgcaaaac tgccccggcg atcgatcgca tccataaccc gtagtgcttc
      901 cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg aacataccgg
      961 gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga agtaaagaga
     1021 ggtcaagaat tatgattact acccgaactg ccaggcagtg tggacaagca gactacggat
     1081 ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa ttgttaggct
     1141 atgcctccct gcgcgtgctt aaccaggaag tgctggcccc aggtgccgcc tttcagccgc
     1201 gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagca gagtatcgcg
     1261 acagcgaagg caatcatgtt caggccagcg ctggtgaggc gttgctgctc tctacccagc
     1321 cgggtgtcag ctatagcgaa cacaatctca gcaaagacaa accgttaacg cgaatgcagc
     1381 tttggctgga tgcctgcccg cagcgagaga atccgctgat tcaaaaactg gcgcttaata
     1441 tgggcaagca gcaattaatc gcctcgccag agggcacagt gggtagcctg caactgcgcc
     1501 agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat ttccagttgc
     1561 atggaccacg cgcgtatttg caatcgattc acgggaaatt tcatgcgctt acgcatcatg
     1621 aagagaaagc ggcgctaacc tgcggtgatg gggcgtttat tcgtgacgag gctaacatta
     1681 cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc tagttgtttt
     1741 tacagggaga tgatgatgag taaaaaatcg gccaaaaagc gccagccagt gaagcccgtg
     1801 gtggcgaaag aacctgctcg taccgccaaa aattttggct atgaagagat gttgagcgaa
     1861 ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc taccgcgtaa
     1921 agacaggtgc cggatgcggc gtaaacgcct tatccggcct acaaaaccca acaacatcaa
     1981 caaattgtgc aattcgtagg ccagataaga cgcgccagca tcgcatccgg cacgataccc
     2041 aaaactggcg tttatctggc cttgctcgcc ataatctcga taatctgccg atccgtttgc
     2101 tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg actgctcaac atcatgcgcc
     2161 acgatcccct cattgccggt cacggcggta tcatccagcg ccattaacac cgctttccac
     2221 gcagccgaag cactggtcga aaccttcatc gcgcagctgt tcgacgctcc atcgcaaatc
     2281 atgccgctga catcgccgat catactgctg atcgccatcg aaatgatttc ataacgccca
     2341 tccaccagcc atgccatccc ggcggcggcc cccattgctg cggtcgttgc ggcacacagc
     2401 gcagacaaac gcggtaactg gttatggatg taaattgcgc tcaaatgcga aagcatcagc
     2461 gcacgcgcca gccgttcatc atcggccccg aagtgttctg ctaccaccac cacgggcatt
     2521 gttgcagtga tcccctggtt acccgagccg gagttactca ttgccggaag cgtagcgccg
     2581 cccatacgcg catcggatgc cgcgctggta cgaatcacaa tggatgaaga gagatctttc
     2641 gccagcaaac cgcgtgcaca ctgtttttcc agcgtcgcgc caatatgcag cccccactta
     2701 ccgctcaaac cttcctgcga taacgcacaa tttagcttcg cggaatcgag aataaagcgg
     2761 atcgccgcaa acgggacttc attgacgaac ttcaggatct cagccagcgt cgttcttgaa
     2821 agcaccgtca gcggcgactc ttgctcgccc tctgtcacac acgcctgctg ggtaaacacc
     2881 acaccattgt gcgtctcaat atgcacaatg ttggtatgcc cgccgacgat ggtgacacac
     2941 gcccacttct caccgttcca gactttggcg cgtgagaaga ggatttcatc gcaaggttcc
     3001 tggatcttaa ctgagacttt ccccgccgcc agcagtgctt tggcatcggc aattgcctgc
     3061 gcagttgcgt ctttcagcac ttccagcccg gcgttagcat ttccacctaa cgctcccagc
     3121 gccgccgcaa ttggcagccc caccattccc gtgccgggaa cggtgacgcc cagaccgttc
     3181 ttcatcagat ttggcgaaac ccaggcttct acacgttcaa ccggaccttc cagttctgcc
     3241 gcagcaaccg ccgccgccag cgccagtgaa atcggttcag tacatcccag cgccggtttt
     3301 acttcctcct gaacggcgag gatgtaacgc tgccataacg gatttaaagt cgaatcaaac
     3361 atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg ccaggaacgg agaaacacaa
     3421 agcaacaaac cagtgacgat aatcaggtac agagacatcc ctttgtattt gtgcaatgcc
     3481 ggtactttgt aaaccagcca cgccgggatc aggcacccta ccatgccgaa aatcgggcta
     3541 cagatagagg tgaagctcaa caccggtgcg ttcagtacga tggcgctcca ggccagcaaa
     3601 atggcgaaaa tcatgatgcc gcgctgaacg agattttcgt taatcttctc ggcaggcatc
     3661 ttgcgacgca ggatgttcat tacgatccct tgcgttgctt cgcgaaagcc taaatagacg
     3721 ccaaagaatg cagtcattac agcaaagata ttgagaatga cgctgaccac tttcacccac
     3781 gctgcgccat caccgctaat aaactgtgcg gcaatcgcca acgcggaaat attctgctcg
     3841 taggctttta ccgcttcgtc atgtcccatc gccagcgtga acgacacggc gtagaaaaag
     3901 acggtgacaa acaaaatgcc aaacgcgata ttcattgccc gcaatgcttt atgccgcgcc
     3961 acttcaattg atttttcccg cgagcgataa gagatcacca tcgggcttaa cgtctggata
     4021 aacagaatcg acgtcagggt aaacggcagc gtaataatgg cgtttttcac cagcagcccc
     4081 agcggcggta gcgaaccgac gttatacaga tgccacattc ccaccatcga cacgcccagc
     4141 gccgcgacca ccagcagctt ggtcagcacc atgccggtcg aaattttaaa taacaatttc
     4201 tcgccgcgtg aggagatcgc caccagaatg caaatcagta ccagaccata aaagggactg
     4261 tctgacagca ccccttccgt cacgccgaag gtatgcaggt aggaagcact atcgttggtg
     4321 atggcggtgg aatagacgaa catccaaatc accagcatta cgaaatagag cgcacctaac
     4381 aggatgcccc agtttttacc taaataaccg ctgatgacgc tcgggtaatc tttacactcc
     4441 ggtgattctg ccagcgtatt aataaacaac cgctgaaaca gatacattgc cgggtaacca
     4501 atcaccgatg agagcaaaaa tacccacaat cccatcaaac cgacctgcac cgggagaaaa
     4561 acaatccccg cgccaatcgc catcccgata ctcataatca cccagccggt gtcagtactg
     4621 tcgaatttga tcgcttctcg ccactcgctc tcactcattc cagcacgacc cgccggggtc
     4681 gaagcgtctg caatgacgcc tttattcgat gcaatttcca taatttctcg ctcaatattt
     4741 tgtagggctt attatttttt tccgagccgc atcaaggcga tatgcggtct acgtttttgc
     4801 aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc agagaaaatc ttcacaatca
     4861 agactttgaa ataacggaga tggataagaa ttttctacta aattaatcgc aagagaatat
     4921 tcattctcta tttgcggcag atcacaaaaa aaaggtgcac atttgtgcac ccaaggatga
     4981 aagctgacag caatgtcagc cacagaccac tttaatggcc agtcctccgc gtgacgtttc
     5041 gcggtattta tcgttcatat ctttgccggt ttcatacatc gtctcgatca ctttatcgag
     5101 tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc gcggcgttta ctgctttcac
     5161 ggcattaatg gcattacgtt caatgcacgg gatttgtacc tgtccggcaa ccggatcgca
     5221 ggtcagccca aggttatgct ccatcgcgat ttccgccgca ttgcatacct gcgccggact
     5281 gccgcccagg agttcagtta accctgccgc cgccattgaa caggccacgc caatctcccc
     5341 ctgacagccg acctccgcac cagagatgga ggcgttcatt ttatacagcg cgccaatagc
     5401 ccccgctgca agaaaatagc gggcaattga ccgctcgttt accggacgac ggaacttatc
     5461 gtaataagcc agcactgccg gaataatgcc gcacgcaccg ttagtcggtg ccgttaccac
     5521 gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg tacatattga tccagtcgat
     5581 gacattcatc ggatcgttag agatgttatc gctggaaacc agttgacgac gcagcgctac
     5641 ggcacgacgc ggcacattga gcggaccagg cagcacgcct tcagtgttca tcccgcgttc
     5701 aataccgtca tgcatcactt gccagatacg ggcaaaccct gcgtcaattt ccgctttact
     5761 gcgcagcgcc agctcgttgt gcatcatcag gccagatata gacaggccgt tgtaatcaca
     5821 cattttcagc agttcacctg ctgagtggaa atcgtaaggt acgggtgttt cgacatcgtg
     5881 cgacaggccg aagtgttctt cttcgacaat aaacccgccg ccgacagagt aataggtttt
     5941 gcttaatagc tcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg
     6001 caacatttcg ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcgcc
     6061 agaagccacc ggcaaccgcc cgctgcgcgt cactaactct ataaatgcag ggatctcatc
     6121 aatgacaaca tcctgcggac tgtttcctgc cagtcccatg atgatggcga catccgtggc
     6181 atggcctttg cccgtcagtg acaacgaccc gtacagatcg accacaatat ggctcgtcgc
     6241 ggttaataag ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc
     6301 cacagtatgc gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat
     6361 atccacaccc tcggattgcc gttcagtgaa gtggagcgga acgaccttgc gaccgtcccg
     6421 ctcacgaggc cttacgcact acgtactgcg atggcttcaa tttccaactt cacatctttc
     6481 ggcaaacgcg cgacctgcac acagctccgg gtcgggtagg tcgcctgatg ttcatcgaag
     6541 aactgcttat agacttcatt gatggtggca aaatcattca gatcggtgat gaacacggtc
     6601 atcttgatga tatcgcccac actcagcccg gcagcaacca cgatcgcttt gacgttttcg
     6661 aggcttaaac gcgcctgatc ttgcacatca gccgggatct caccggtctg tgggcaaacc
     6721 ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa cgccctgaac ataagggccg
     6781 attgcgcctg gggcacgttg cgtttcgata atctttttca tacatcctcc ggcgtcagag
     6841 cgcctgggta aaggtacgtg aaataacatc ctgttgctgt tcacgggtca gtgcgttgaa
     6901 gcgcacggcg tagccagaga cacggattgt caggttagga tatttttccg ggtgctcgat
     6961 ggcatccagc agcatttccc gattcattac gttgacgttg aggtgttgac cgccttcgac
     7021 atccgcttcg tggtggaaat acccatccag caggccgaca aggttggttt tacgtactgg
     7081 atcttctttg cccagcgccg cagggacgat ggagaaggtg tacgagatcc catctttggc
     7141 gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc acggcgcctt tgcgatcacg
     7201 accgtgcatc gggttagcgc ccggcgcgaa cggtgttccg gcgcggcgac cgtccggcgt
     7261 gttcccggtt ttctgaccgt acaccacgtt agaggtgata gtcagaatcg attgagtcgg
     7321 cacagcattg cgataggttg gcagagcttt aattttcttc ataaagcgtt caaccagatc
     7381 gcaggcaatg ctgtctacgc gctcgtcgtt gttgccatac tgcggatatt cgccgtcgat
     7441 ttcgaaatcc accgccaggc cgttttcgtc acggattggt ttcacacgag catatttgat
     7501 ggcagacagg gagtccgttg ccaccgacag gcccgcgatg ccgcacgcca tggtgcgata
     7561 gacatcacga tcgtgtagcg ccatcagcga ggcttcatag ctgtacttgt cgtgcatgta
     7621 gtggatgata ttcagcgcgc tgatgtactg taccgccagc cagtccatga agtggtcgag
     7681 gctatccatc actttgtcgt agtccagcac gtcatccatc aacggagccg ttttcgggcc
     7741 aacctgaatc ttcagcttct cgtccattcc gccgttgatt gcgtacagta gcgttttcgc
     7801 caggttagcg cgcgcaccga agaactgcat ctgcttacca atcaccattg ggctgacgca
     7861 acaggcaatc gcgtaatcgt cgctattgaa gtcagtacgc atcagatcgt cgttttcata
     7921 ctgcaaagag gatgtgacga tagacacctg cgcggcatat tttttgaagg caatcggcaa
     7981 ggcttccgac cacagaatag tcaggttagg ttccggtgca ggccccatag tgtgcagggt
     8041 gtgtaaatag cggaaggagt ttttggtcac cagggttcga ccgtccagcc ccatcccgcc
     8101 gatcacttcc gtcgcccaga tcgggtcgcc ggagaacagc gaatcaaatt ccggcgtacg
     8161 caagaagcgc accatacgga tcttcatgat gaagtggtcg atcagttcct gtgcctgctg
     8221 ctcattgagt acgcctgctt taaagtcgcg ctcaatgtag atatcgagga acgatgccgt
     8281 gcgccccagc gacatcgcgc cgccattttg cgatttcact gccgccaggt aagcgaagta
     8341 gagccactgc accgcttcct gtgcattctg cgccggacgg gagatgtcaa aaccgtattt
     8401 cgccgccatt tcttgaatct gcaacagcgc acgacgatgt tccgccagct cctcacgcag
     8461 acggatcgta gcttcgagat cttcgccttt ttccagacgg gactggagat cggcaaactg
     8521 cagttcgcgt tcacgtacca gataactgat gccgtaaagc gccacgcggc ggtagtcacc
     8581 gatgatacgc ccacgaccat aaccatctgg taaaccggtc agtacaccag atttacggca
     8641 gcgcagcata tccggtgagt aaacatcaaa tacgccctgg ttatgggttt tacgcagatc
     8701 ggtaaagatg tattcaaatt cgctatccat ttcacgacca tacgcgtgga atgaacttct
     8761 aatcatatta atgccgccga acggatgcag cgcacgcttt aatggcgcat ccgtttgcag
     8821 acccactatt ttttccagcg gctggtcaat atatcctgca tcatgagcgg taattgtggt
     8881 ggcaatattg gtatcgaaat ccaatggcgc gtgagtcgca ttttcgatac ggatgccttc
     8941 catcactttt tcccacaatt ccgtggtggc aggcgtcgct tcggcgagaa aagattcatc
     9001 accttcatac ggtgtatagt tatgttgaat aaaatcgcgg acattaattt cgtttttcca
     9061 gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc ttatcgctgg tatcaatatc
     9121 taccttcatg aaaaataatc tctctacaat acttcaacta aattatgcaa attctgcggg
     9181 cgcgttcact ttgcctaaat gaatggcatc caaagcaatc attttttctt cgttagtcgg
     9241 aattacggcg caaatgacac gcgcattttc actggaaaca attcgctctc caaaagagtt
     9301 agagcgatta ttcatttctg tatcaatctc tacgcctaat acagctaaat gttccatgac
     9361 cagacgacga attaaacttg aattctctcc tattccgccg gtgaatataa ttccatccag
     9421 gcgatgtaat gaagcagcgt gtccggcaat atgacgggca attcgatgaa caaaggtttt
     9481 aattgccaat tgcgcgcgtt cgtgaccttc atgccaggct ttttccagaa cacgtaaatc
     9541 cgaagaaagg ccggaaatac ctaataatcc cgactcttta ttcacgacgc gttccaggtc
     9601 acccaggctc tggttggttt ggctggcgac ccaggacatc gccccgaagt cgacatcacc
     9661 actgcgggta cccatcatca agccttccag cggcgtcatt cccattgagg tatcaacact
     9721 ctgaccgttg cgaactgcgc agattgacgc gccattgcca agatgcgcca caaccaggcc
     9781 ggagtcatct tccgccagat tcagcaaaga atgcgcgcgc tgggaaacat agcggtgcga
     9841 cgtgccgtgg aaaccataac ggcgtacacc taactcttca taatatttcc acggcaggcc
     9901 gtataaataa gcttccggag ccatcgtctg gtggaaactg gtatcaaata ccgctacctg
     9961 agttacgccc ggaaataatt gctgcgccga ttcaatacca cttaaattgg cgtaattatg
    10021 caggggtgcc agtggagaaa cgcgacggat attatcaatg acttcatcgg taataatggc
    10081 ggactcggta aaaatactgc cgccgtgagc gatgcggtgg ccaattaagg ccacactgtc
    10141 atttaaattc cgtttttcca gttcaaatgc aattgccttc aatgcacctt cgtagctgtg
    10201 gtgagccagc ggtgctggct ctcccccatt tacggataag aatgcatttt ccgagttaat
    10261 accgtcggca atacctgaca ttaatacttc acagtcgctg gcattgagta cggaaaactt
    10321 aatcgaagac gaaccacagt taataaccaa aacaaccgga aattcattca tctcttttct
    10381 catcctgagt tacggattaa aacagtttgt atacgatgtt caggatagtc agcagaccaa
    10441 tcacggtaac aaacacgtta tccagacgac cacggtattt cgccagagac ggcgctttac
    10501 gaatggcata catcggcaac aggcatagca gtgatgcgat aatcggtgcg cccatggctt
    10561 caatcaggtc gaggatgttc gggttggcgt aggcaacaac ccaggtggag cccatgatga
    10621 agatcatgct gagagtattc agtttaccca gcgacacttt ggttttgtca cctttataac
    10681 cgaacttcag aatcaggcca ttcaagcctt ccagcgtccc cagatagtga ccgaagaaag
    10741 atttgaagat agccacgagt gcgatgatgg aagccgcata ttccagtgta atcgcgaacg
    10801 ttgttttggt accggtcatg gacgcaaagt ggttagccag ataagaaagc actggaatat
    10861 tctgcgcttt ggcttccgcc atgttggccg gagacagcgt aaacaggcag ctaaaggcaa
    10921 agaacatcac cactgcaacc atcagcatgc tggcacgaga aatgatttgg gaacatttac
    10981 gttcggtgaa gtcgcgaccg aagtctttct catactcttc acgtttagaa accacgaagg
    11041 aagagacgat tggcgagaag ttaaaggaga aaaccatgat ggaaatcccc agccagacag
    11101 tgatcaggat accgtcatga ccggttaacg acagcgaacc gaggtcaacc tggtcgataa
    11161 ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag caccaggctg gcgataaacg
    11221 gccataccag gtagctcatc actttaacca tcagatcctt accaaaccag atgacgaaag
    11281 ccatcagcag caacaggaac agcgccacaa agccgcgatt cagcggtgca aagccgagct
    11341 ggttttccca gaacgtcata aaggtattgg taatagtaac gccataaatc cacagcagtg
    11401 ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc gccagtttta ccaaaatgct
    11461 cttccaccgt ttccgtaatg ttgccggaag ggttagagcc agaaagacac agacgcgcca
    11521 gtgcccggtg gcaataaaac gcgatggggt atgctaatac caacatcaga agaatcggga
    11581 tcagtccgcc aaaacctgcg cggataggga agaacagcac cccggcgccg attgccgtac
    11641 caaacaagcc taacgtccat gtggtatctg atttacgcca ggacgattgt tttgtctggc
    11701 tggatacaat gctatctgaa gtactcatat cctatcctca acgaattaat taagcgtcaa
    11761 cgaaaccggt gatttgagag acgcgagaaa gatcgatatt gccgccggaa ataatactga
    11821 cggtttttct gttttgaata tattggtcta atttaccgct taataatgca gcacatgcca
    11881 gagcgcctgc gccttcggtg acgactttat tgcgctgaat taaggcaatc atactgtttc
    11941 tgatttcgtc ttcgctgacc agcacgatgt catcgactaa ttcacgaacg atttcgtaag
    12001 ttaaattacc cgggcgggag acatcacaac catccgccag ggtgccggta gttcggtgcg
    12061 tagttatttc tccggagtgg aaagaagccg ccatgccgtg aacgttttct gactgtacgc
    12121 caataacacg aatagtcggg ttaattgatt taatcgctac cgcaatacca gcaattaaac
    12181 cgccaccgcc aattggcaca atcacattat cgacatcata gagatcttcc ataatttcca
    12241 gaccaatcgt tccctggcca gcaatcactt tcggatcatc gtaaggtggg ataaaaatac
    12301 ggccttccat ttcgacaatt tcgctcactt tagcaatagt gtcgttgaag ttatcaccat
    12361 gcaaaacgac ttctgcggag tagtcgcacg ttgccgcgac tttggatttc ggcgcacctt
    12421 ttggcatcac cactttaccg tcaataccca gcatcgcaca ggagagcgaa accccttgcg
    12481 catggttgcc cgcagagcag gccactacac ctttacgttt ttccgcatca gtcagtgaac
    12541 ttaatttatt aaatgcgcca cgaattttaa atgaaccggt acgctgcatg ttttcgaatt
    12601 tgaggaatat ttcacctttg caacgttcac taaaatagtt ggagcgaggc atacctgttt
    12661 tataaattcg cccagccagt cgttgttttg cttcaatgat gtcatcaata gcaaccggga
    12721 gatcgtatgt aatatgcatt ataaaacctc ttagcctgaa ttaaatatgt aggtaac
//
LOCUS       DAZKJR010000140        12757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKJR010000140 DAZKJR010000000
VERSION     DAZKJR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.141"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1040)
                     /locus_tag="ACHOWS_004139"
     CDS             complement(<1..1040)
                     /locus_tag="ACHOWS_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0049634.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAA"
     gene            complement(1047..1388)
                     /locus_tag="ACHOWS_004140"
     CDS             complement(1047..1388)
                     /locus_tag="ACHOWS_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049635.1"
                     /translation="MSRIYGRMKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            complement(1393..2814)
                     /locus_tag="ACHOWS_004141"
     CDS             complement(1393..2814)
                     /locus_tag="ACHOWS_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFQ0049636.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGVEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            complement(2828..3802)
                     /locus_tag="ACHOWS_004142"
     CDS             complement(2828..3802)
                     /locus_tag="ACHOWS_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049637.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCTVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            complement(3799..4194)
                     /locus_tag="ACHOWS_004143"
     CDS             complement(3799..4194)
                     /locus_tag="ACHOWS_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049638.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            complement(4330..4962)
                     /locus_tag="ACHOWS_004144"
     CDS             complement(4330..4962)
                     /locus_tag="ACHOWS_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0049639.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            complement(5154..5432)
                     /locus_tag="ACHOWS_004145"
     CDS             complement(5154..5432)
                     /locus_tag="ACHOWS_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049640.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
     gene            complement(5812..6000)
                     /locus_tag="ACHOWS_004146"
     CDS             complement(5812..6000)
                     /locus_tag="ACHOWS_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFQ0049641.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQDECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            complement(6378..6755)
                     /locus_tag="ACHOWS_004147"
     CDS             complement(6378..6755)
                     /locus_tag="ACHOWS_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0049642.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(6760..9456)
                     /locus_tag="ACHOWS_004148"
     CDS             complement(6760..9456)
                     /locus_tag="ACHOWS_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFQ0049643.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRVREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            complement(9594..9986)
                     /locus_tag="ACHOWS_004149"
     CDS             complement(9594..9986)
                     /locus_tag="ACHOWS_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFQ0049644.1"
                     /translation="MTVMVAVFILPGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            complement(10022..11509)
                     /locus_tag="ACHOWS_004150"
     CDS             complement(10022..11509)
                     /locus_tag="ACHOWS_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049645.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            complement(11499..12407)
                     /locus_tag="ACHOWS_004151"
     CDS             complement(11499..12407)
                     /locus_tag="ACHOWS_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049646.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            complement(12407..>12757)
                     /locus_tag="ACHOWS_004152"
     CDS             complement(12407..>12757)
                     /locus_tag="ACHOWS_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFQ0049647.1"
                     /translation="REILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVE
                     GRDNWQVQLDLVADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQR
                     LEAASPAAGKGDNQ"
BASE COUNT         2804 a         4138 c         2881 g         2934 t
ORIGIN      
        1 gctgctaccg cactgcccgc cgttcccgca atccgggcca ccgcattcat ggtcgtcata
       61 tctgcattgc caccatagcc acgatacacc tctcccccct gagagagcgt caggtttgcc
      121 ggactcacca gacgcttgat cacctcctcc ttataatcca cctgactact gccaatcatc
      181 ttcattgcag ctgacagacg taaccagtaa tgatcactga cctcctccat gacccgtttt
      241 ttcagcccca cactactgtc actccaccac tcccggcatg tcggatagcc tccccgcccc
      301 gtatccggtc gtccgctgtc acgtgctgta ttccacggga acgcagcccg cggcatcttt
      361 gactgcagcg tcccgtaatc cccctgcatg aatgtggcgc tccctatcca ggagatatca
      421 tcaagcactt ttttatcttt actctgcccc tgatcacgct gtttccacat atacagtgcc
      481 agtgaataac agtcgttcgt aaagtcctgc agctcatcag caagtgccct gttttctata
      541 aacgttcgct ggatctcaaa acgcaactgg cgcaaatcag gccggcacgg aatcgaagcc
      601 actgccgcct gggtgacacc tttcgacaat ttatgaacca ccacccacca taccggggca
      661 gcagcagtct gatcccctat actgctgatc accccactgt accccgtctc tccgggctct
      721 tcaggtgtcc ataccccaca ggtctgtgcc ctggatttat cgaatgacag cgtatccagc
      781 gtcacctgta ccagaggaac acagcaggat atcatcacaa aaaaagcacc atagagaaca
      841 tgctcaatac gcggcaggga aagcatccct ttgtttccct catccgcacc ttcttccctg
      901 actttcagcc agatcccgat gatcctgaat gccagtggca gggcaaacag tcctgtgctg
      961 atcaacacat tccacagacc gttattcacc acccagccca gcagagtcag aaaatattcc
     1021 agatagttat ttgtggtcat gattgctcaa cgccccggct cagcaaaaat ggcgtattca
     1081 cacagcagga taaacagaac actgactgtg gccatacgca gaaaagaagc ccggtattct
     1141 gccctgaacc ccggcgcatg ccagatcctc caccccagcc atccgatcgc actgtaaaga
     1201 cacagacgcc atcccagcca gccataacgt gtctcataca tccactcccg cagggccgca
     1261 aatccttctg cgtgacgtat accggtatcg gccagccaga gggaaaacac ggtaatccct
     1321 gtcagcaaca gcgtatacag ggcgaatacc ttcatcctct ttttcatccg gccataaatt
     1381 ctgctcatcc tctcactcct tatccggggc atcgagagaa tggatacggc tgtcagcatt
     1441 gtcagcctgc tggatcatcg ggttattccc cacacgctcg ttatcccgtt caatgatggt
     1501 cagaacactg ttacgcgcca gatcccgctt cagctccatc tcgtttttca gcgccataat
     1561 ttcccgatcc agcgcctcaa tacggcgttc cccctcttcc agtgcagcag gctgagctgc
     1621 agcatacggc tcggccatcc ccgtgagcag catcctgcgc atcagcagcg ccgtttcaac
     1681 ggtatccgcc atggccagtt ctcccgccag acgcgacgtc agggcaccat tgtccggatc
     1741 acgctgcagg gcacggatca ctcccgctgt cacagacaga ctcccggctt tcagttttgc
     1801 cagattcccg gctgtcggct tgtcacgacc attgaccata cctgccagca gttccgtgtt
     1861 aacccgcgtc gcttcttcaa gcatcggcgc aaaccccgtg ccggcaatac tggtccccgg
     1921 ctgctgatca gcatccccgc tggtacatgc ctttgcatca ccacaggtcc gcatggattt
     1981 atcccccaga accttcacca ccgcccgggc agcttcctct gcattacggt atttcatgca
     2041 ggcactcccg ttacagttcc cgggactgac actggatgta ctggtcgaag gcagactgtt
     2101 catcatattg aaaccggcag ctgtcagatc atgtgtcggc cggatagcag actgtcccgc
     2161 tccaccacgc tgctgccctc cgatccagcg ttgcccctcc accccggtaa cctgacgacc
     2221 tgcttcatcc cctttgatcg catcaccgtc ccccgtattc acaatttttt tgtactcctc
     2281 gagcatggcc gcctgcgtcc acttattgct gtccgtgaac tccatcatgc gtttcgccat
     2341 gttctgacaa ttgagctgcg ctttgtcgaa tgccacattc gcctgcagta caccgttggt
     2401 cagcatgtca tacagccccg gattagcccg ctgtatcacc attgccggca gactcgccac
     2461 ggctccgctc gccccctgaa tcacatcccc catcaggttt ttgaaaccat cagtgatccc
     2521 gttcagctgg ttacccactg ttgttttcag gtcaaaattg ccacacatca gatcactgct
     2581 ccacccgaga tccaccccaa gtttctgcat attgctgcgc gttgccggct gcgatatcac
     2641 cgagccccct cccagggtat aaaacagttt gtctgatact gcgccgtcca cggatttacc
     2701 gtagccaaac gcactgtcat tcactttcgg cagggacaaa aagtttccgt cagcctgtac
     2761 cgtacccgca caaagcacca cagccagaac aggtatcgtc attttctgtt tcatcacttc
     2821 cccttactta aaaattcgta ctgcccagga atgtctgtcc ccgtttcttg cagcaactgt
     2881 agggctgcca cagggcaaag gcagcattac ctgtttcagc cgcttcatgc tctccgtcag
     2941 ggaagactgc gcaggacgtg gagactgaag gcgtcagtct ctgccattta tggttccggg
     3001 tgcccgtatt ttcctccacc ggatccggtg gccagtaacc gtccttcttc cctgctttca
     3061 ccggctgata gacatgtggc tcaccggtgc gggtgataat gtcagccacc cgctgggcca
     3121 ccaccgcagc agctttatca tcatctgtct gggtcacgaa cccggagcgg ggatacacat
     3181 tgccccacat attctcttct tcctggctgc ctatttcgcg tttaccgggg atcagggcct
     3241 ccgggtacag tgactccggt agtcctgtac gccaggcaat actgtcaagc gtactgagaa
     3301 aataaggaac aaaaggtgtc accggtgact cacaggaata tcccggcacc atccctccga
     3361 taagcgtggt ggccgggtgc cctgttgcat cggcatattt aaaacggatt gacgaacgac
     3421 gttccccctg taccttcatg tctgcggggt tccctcctcc tgctgacaga ccggcaatac
     3481 ctttgcttat ggcattctcc ggaccaccgg ctgtactgct gaccgccgac atttcacgcc
     3541 agggatttcc gccaggtgcg gtataggtgg aaacaaccac ttccgggata taatgggaaa
     3601 ctttcactga tgttctgacc gtgcaactga atttggtgca gaacagccag tagcaaatcc
     3661 cgctgacccg ccagctgata cagctctgtg aggctgcact ggccactatc tgcgcggtgt
     3721 tcagcgatgc ctctgccacc ggcaataccg ccatacttaa cagcagaaca ccggcgcgat
     3781 gacgtgatgt gcaggaaatc atggctgccc tccccctgta cgcagttgtt ctgccagcac
     3841 cacgtctgtg gttccgtaga ccacttcact gtcatcaaaa accacagccg gatatttttt
     3901 cagtccaagg gaccaggcat gtgctaatgc acggtagtgt tcagcaagcc ctgcctgaaa
     3961 cgactgccat tcaggcgact gcatccgttc catgacacgg gcagatgctt ctgcaggctc
     4021 tgcgggaagt accccgaaaa atgtatcctg cgtctgctca ggagcatcaa gcaggacaac
     4081 acgaacacct gctggcgggt tcatagggcg ctggtgcgaa tcggtataaa ttgttgtacc
     4141 ggcagatgca gccaccggta gtatcaggaa ggaatacagt aacagtcggt acatgatgcg
     4201 actccgtcag acaactggta tctgacagcg tgcgcaaagg caaagggaca atcagcaata
     4261 aattcaaaac cggatgataa aaattaagcc tcttcctctg aataacgcac actctgcatc
     4321 tgaatcaaat taatccggtt gcatcacagg aagccactga ttcggggtaa taccataata
     4381 acgcctgaac acagaaataa aataagaagc actggcatag ccacacaact ctgccacccg
     4441 ggaaatacgg ttttctcccg cagacaaaag ctgagcggca tactgcattc tctctttcag
     4501 aagaagctca ctgaaggtcg tcagctcttc ctgaagcttt ttcttcagta aactctcact
     4561 tatatataac agactggcca cttcacccag catccatgga cgggacaaat cagcactgat
     4621 aatattcttc acccggacag tgcagctccg gatacacccc gtgagcaggg cgtggaaatc
     4681 cggatgttcc tcaaacagcg ccagactgga aaaagccacc gcatccctga agtacaccgg
     4741 cgatggttca tctgtaataa gttgtctgat cagacattcc gccagcacgg acatctgtgg
     4801 ccggatctgt aaaagcaaag acgacgggct gactgccata ccggataatg gcgtgcagaa
     4861 ccgcagatac cgcctcacca cctcctcact gaggtcagcg ataaggaaac gtccggtatg
     4921 agcctgaaaa gcagggcgca gatgcccggc aatcagaagc attgtgccgg caggtatgac
     4981 agagttcctg tgctccagac aaagcattgt ctgtttgaga gccataatga cagcgcaggt
     5041 acagggcatc ggatatccgg taaatatgct gatttattaa agagacagac atgccccgta
     5101 tataaaattc cgtgtcaccg ggtgctgaaa aaaactgcac cggcggcgct cttttactcc
     5161 tccgttacgg atatttcagc ccccttttca ggagcggtat ccttcacctc gctgagcccg
     5221 gactgagccg cttcagaaac ctctgccacc tttccggcag cctgcatttt cacttcctcc
     5281 gcggcatcct tcacatcact gagttgagtc tgcgcagctt cctttatatc tgccgccttc
     5341 ttacttacat tctcctttgt cgcatcatca cagcccgcaa ccagaaatgg tgcggaaata
     5401 cataacgcca gaattgtttt ttcagcatac aatctctcat tattaagtgt taacgcagaa
     5461 cagcttatat ttcgtccccg ggaaatggtc aaaagacaac cagaacatca cagtgtcagg
     5521 gcacactccc ggtatatgct tttataacca taacatgctg ttcctgatat taccggaaat
     5581 gtgcccacta catttaacct gttttcagca ccgggtcatc ggctgtgctg tattttttat
     5641 tcccgtcaca gaaacacact attaccgttg atttcatgac gaataaaatg aataaattct
     5701 cccgtggtct ttaccaccat tattatacca ataacaccat ttacacagat acactccggg
     5761 actatctgtt tttgccccct ccgactccgc cggaggtttt tattttcata ctcaggcctt
     5821 caccacttca caccggaagt cctccaccct gacgccaata taccgtcccg gctcttttgc
     5881 gggcgcaaac agtttttcac attcatcctg agtcattttt tcatcctggt aacgcagcca
     5941 ctgaaccggc atattgcctg gtttgattat tgtcgcagtt attttatatc gctttcccat
     6001 actggctctc atctcaacaa caaaatcacc ggctgagcca tcactccggt aatgcatgcg
     6061 aaaaaaagtg agcgtctcag caatggctca tgcggtgatt ctgtacggta cccgcacaaa
     6121 atacgggcac caacagcgtc gcagaataca acgaaggcat cagggcagca cctctctccc
     6181 gctatttttc agagttcttt accctcctcc tgacacggga gtacaaccct gacagcatcc
     6241 gggccagtac attcgcaagc ccccatgaaa acacagccac acccacagct cccgccagaa
     6301 acagcacagg ttcactggca atttgatctg acagcgatgg atacagtggt cccggaaaat
     6361 cgatatcgct cctcatatca cccctctccc agccacaaca gcagatagaa caaatccgcc
     6421 gcatcagtca ctgatatata ccagcgggga tgaatatact cctcctccga cggccagctc
     6481 tcatgcccac tcacagtaag cagaatatgc acatactgac cctgctgacc ttccagcatc
     6541 accgccacac tgctgttcag acccaccctc tgtggctggc aacggataca ctccacccgc
     6601 cccagttgcg ccgccagatg cccgcgaaga cgttcaagat tatcccgtgt ggtgcaactt
     6661 atccccggcg caccctgtgg cggcagaaca aacacacttc cggcggggtg aacagcagac
     6721 accacttccg gttccggata cttcagacac agcatagtct cactccctgc cagccagacc
     6781 acgtagcccg tccagctttc gtgccacatg aaacgctgct tccagttcgc tgcactggtg
     6841 caccttcatc agggcgcggc gttcagcctt ctcttctttc tccgtcatcc ccagagccag
     6901 atacagactc ggtggcacaa cccggaacaa cgcttctgtg cgcttcgcca gtaccacccc
     6961 ctccgtataa cagccggaca gtttactggc tgacagcagt accgccttct gctcctccgt
     7021 cagggattta aaacgggcaa tattgttcac ttcctccggc ggcatggtca ggcacaccca
     7081 ccactctgcc atattcagca tcttctccga gacatccgga taatccttca ggttctgcgt
     7141 ggccagccac agccaggccc ccagcttgcg ccacattttc accactttgg tcatatacgg
     7201 ggacaaaagc gggtttaccg tcacgatatg cgcctcatcc accacaaaca gaatatcccg
     7261 tcccagatac tgatcgcgct cggcaatgtt gttaatcata ttagtcattg acaccatggt
     7321 cagtgccatc tgggcttcat aaccttcacg ggcaagatgt cccagatcaa tcagggtcac
     7381 atccgcttcc ggccacagtt ttccttcccg gttaaacagc tccgcttcaa aactgcccgg
     7441 ctgggtgaac atccccagcg actctgccat ctcagcagct ttagcccggc gaacatcatt
     7501 acgctgagtg tcccgcgaaa tttcccacaa cgcactctgc agatccaccg gcagcatctg
     7561 acgcccctcc cggtacgtgg tgtgcgtggc catcagcagt gcctcacgga tcatcgccct
     7621 gtccgcacgt ttgagcgcgg cttcttcttt cggatcccct ccggtgatca tcatgcgggc
     7681 agagatttcc atctccccca gaatgtcgcg tttctcttcc tcttcatccc cctcatcttc
     7741 atcaagatcg ggcagtgaat gctcatccac actctgcagc gtctctccct gctccaccag
     7801 ttgatgagca tccgcaaatg gaggaagaca cacccccgtt cccggtttga cactgatttt
     7861 attaaccgaa agcccgagac tctcaaaata atccgccagc agaccaaacg agttcccggc
     7921 ctcagcgata aacagacggg gacgatgaac tgccattatc tgcgacaacg aaccacacag
     7981 cgtggccgat ttaccggctc cggtaggccc gaaaagcagc agatgtgcat tctgggtccg
     8041 gtccagcttg ttcagcggat caaaggtcag cacatccccg ccacggttaa agaaactgaa
     8101 tccgggatgc cccgtaccgg tttcccttcc cgtcaccggc aggagccctg ccagatgctg
     8161 tacccacatc agacgcgtgt accactgttt tttatccgta tcaggatcaa aacacatggg
     8221 cagcgcgcgc agccagctgt tcagcggtcc gacatcaaac tccgggcgca ctggctgtaa
     8281 gcccgcgccc agcagcaccg tcgtcagttc aacccgtctg cgcttcagac tctccatatc
     8341 ctctgcccgc agcagaaacg tgatcccggc acggtagagc ttgtgccggt ttcccagata
     8401 tttcttcacg gtcgccacat cctcccggac acgcagggat tcactgttct ccccgaccgc
     8461 atttttcgac aacctcgtga agcgctcttc cagtgtgtcc tgtggctgga ccacaattgt
     8521 catacacagc accgttccct ccggaaaggt gtccatcagg gcattcactg tcttttcgcc
     8581 ccgggcctgt tcccctgtga tggttcccgg ctccggtggt gtgcgcagtt tctccaccac
     8641 aacagcggca tgcggaagcc cgtccagcca ccacacgccg ttttccggat cagataccgg
     8701 tggggtgaac cagaggcttt ctgcaaaatc ggtcatcacc ggcatcattc cctccggtgt
     8761 ctcctgcggt tctgcacggg atgccatacg gtacagaata tccctgtcca cccattccgg
     8821 ccggggattg aacagcctca gcagccagcc atgcacctgc tgcccgttca tccgtgtaca
     8881 gcggacgcct gcgccaccca gcgcccccac gacccgcgaa cagacctggt tcagcattgc
     8941 caccggcggc atcggatcgt ggttgttttt cccgatccac cggtaaatca ccatcctcgt
     9001 gcgccgctgc tgaccacgcc atggctgacc tgtgaccagc gtatccctga acagaccttc
     9061 cggtcgggca atccccttca gatggcgctc catttccctc agccaggcct ccgtaaatga
     9121 ggagccttct gcatgaggtt tcacgtaccc cctgagctgg tcaacatacg tgtccacgtc
     9181 gttttcatcc tgacagaaga actgcaccac ccagggatgt gagtcatatt catcaaaact
     9241 gtcctgcagc gcatcctcca ccgtatcccg gatttgctcc agacgctctg cggtccgccc
     9301 ttccgtagcg gccggactca cctcatacac ggcaccaacc gacaccccat catccagcag
     9361 caggcactgc tcttcgtcca gatattccac ccatggcaga aaatcaatca ccgacggaca
     9421 ggcatggtaa accttcgtct catcctgtcg ggtcatcttc cccggccttg acagtggctt
     9481 tcgtacctga ggggccactc tttctttcat atccggagat accgcttctc cgtcatgctg
     9541 cgacaccggg gaagaaaccc gtttttttgt gtctgtaaac agggaaaatc gcatcacaac
     9601 gcctccgttc gttcacccgg catggcatac tgcgtccggc tgtaaaaagg aaatactgtg
     9661 ctgtaccccg gaaccggtga cgtaccggaa gccagatgcg gaaaaacata catcaccatg
     9721 tcaggattgg gcagccgcgg aaactgctga ctgatctcac tttcctgtgt ccggctgtag
     9781 cttatggctc cgtgcgcatc tgcctgctgc tccggcattg tcagcggacg ccgcagggtg
     9841 tccctggccg ctgtcatgtg ccgcgaatgt cctccgcttc ctttcccctg ccagatctcc
     9901 agcatactct gttcacccgg aggcagaagc tcctctttcg atgtcgagca gccgggcaga
     9961 ataaataccg ccaccatgac ggtcattaca gataactgtc gtttcacggg atctccttac
    10021 atcagtccag accaccggta ccgtggactt cccccggcag agtgaagtcg taacgtaccc
    10081 ggcgcccctg atcttcataa tcaatggcca gttgacgcgt gatatgcacc gccagcgtct
    10141 gccccggggg aacataaatg gcatcaaacg tctgcccgta tcgctgcttc acccattccg
    10201 cggtttcatt catcccgccg gacagggctt ttccaagtgc cgcctctccg gcactgccgg
    10261 tcagtgttga tgtgatgcca ttgacgttgt tctgtgaggt gtactgcccc tcactcagtg
    10321 catcacccgc agcgccggct gcagacaggg caaatattgt cggcagatac gtggaggcat
    10381 tggatttacg ggttccgctg atacacggga tcccgtcatc atccgaaatc cagccaatgc
    10441 ctcctgtact gctgttattt tttgcctgtg cgttactgct gccgttcacg tctgatgacg
    10501 gcagcgtacg cactgtcccg tcggcaaaaa caaacgtgat gctgttcacc tgcccacgga
    10561 cacaggagag cgtccagtca ccactcgcag taccggacac cactgcacct tccacatccg
    10621 gcagctctat tccgtttgcc gtcaggttat ccttacccac caggatcttg aacggatagg
    10681 gatccgtcac ggttccgttc accggaaccc gtcccagaag cgctgtcatc gcccggctgc
    10741 ctgccagcgt cgagttctcc ggcagggtat acactgcccg ggtgctcgcc ttctgctgtt
    10801 cattgctgta tcccttcacc tgctgttcat aagccgcttt ctgacgggtc agtggattgt
    10861 catccagaaa agagactgga aaacgcggtc cgctcttatc ccccaccgac gacgcttcat
    10921 cctgcacctg atcggccggt gtcacccaca tcagaccatc cgttcccggt gaggctgccg
    10981 atcctgtcac gctccccagt ccgtcaaggc ccagacctgc tggaatatcg ctctcctgtc
    11041 ctccggcgcc ggcatcagca ctctcctgct gacgcttcag ttgctctgtc agtgactgaa
    11101 cctgtgtcat taacgcgttc tgttcattcc ggaactggcg ctgcgactgc tcataatttt
    11161 tctgcaatcc tgccactgcc tcactgacct gaccggaaac atccgtgccc cgcttgcgca
    11221 gcatttcatt ttctttcagc aacttttcat tctgtttgtc cagtaccagt tgccgctccc
    11281 tcacctgatt catacgccca atcagtgtgc gcagggtatc ttcaggtgtg tctccttcca
    11341 cgcccagggc cttcagttca tccggtgaca gtccggccag cgttcctgag ctttccgcag
    11401 acgagggctc cccggtatct cctccacagc tttttaatcc cgtcaggatc cccgccacca
    11461 ccacacaggg caccacatac ttcacgatcg tattggattt aatctgcatg cttcacctcc
    11521 ccccgtgaag gaatgcgacg ggaggcaggc tccgcgataa acgcctgttc cggccgttct
    11581 gccgtcacca catagagcgt ggtggtatcc tcaggggtac ctgctgcacc cagccagcgg
    11641 tgctggaaag tcgcggcaat gaattttccc tgaagtgccc gcggatcaag gataattttg
    11701 cggcttaccg tactcgtcag tttcagtgca acaactgcac gcccacccgc agcccaggct
    11761 gccagtggcg tgactgtcag tggctctgac ggatagagtg tggtgatttt cccgggcaga
    11821 tgtgtattaa ccggccggat acccggtacc gcttccagcg tccgtaacgg tgcgtacagc
    11881 gactgtgccg catagcgggt caacagaacg ggtaaaggct ctgcatactt caccggctga
    11941 cggagggcct gcgagccgtc atccccgccg ggtaccacct ttttgtgttc tgcagagcgt
    12001 gcagacgccg ctgtgccagc ctgactgacc gtactgacgc ttccctcata cacaatccgt
    12061 acgggctctg tgggcccttt ttctgctgct gtcacatcca gcagaataat ttctccgctc
    12121 tccacatcct gcagttgcag acgcgtctgc ggaaaaacac tctctgcttt cagatagact
    12181 gccccaccgg tactctgtac cctcaacttt ccgttcagtg ctggcgggaa gcccacccgc
    12241 acatttttat ctgcaaaaat aacccgctcc tgtcctgcca tcagggtaac gggtaaggga
    12301 atgcgctccc atttcatcag ctcatcagcc cgtgcgctca gtggtaaaac cagcagtgac
    12361 aggaccatca caatactacg gtataaacaa cgttcagctc cgttcatcac tgattatccc
    12421 ccttcccggc tgccggggag gctgcttcca gccgctgtgg tgtactgctg tagcagtcca
    12481 gcgccagccc gaacggatta cgttcaggat ccccctccat gcgcaccacc tttatcggat
    12541 aacggacaag tgcccgtttt accggctcag catgaaaata ctcatctgcc accagatcca
    12601 gctgtacctg ccagttatcc cgcccttcca ccgtcaccgg atttttgttg tacctgcccc
    12661 cgtagccatg ttccggaatt tcatacacca cgcgcacacg ctccagcagc tcccccgcat
    12721 cactgcgacg gcgggcatca ctctccagaa tctcccg
//
LOCUS       DAZKJR010000141        12732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKJR010000141 DAZKJR010000000
VERSION     DAZKJR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.142"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            257..1771
                     /gene="caiT"
                     /locus_tag="ACHOWS_004153"
     CDS             257..1771
                     /gene="caiT"
                     /locus_tag="ACHOWS_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFQ0049648.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1802..2944
                     /gene="caiA"
                     /locus_tag="ACHOWS_004154"
     CDS             1802..2944
                     /gene="caiA"
                     /locus_tag="ACHOWS_004154"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFQ0049649.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3072..4289
                     /gene="caiB"
                     /locus_tag="ACHOWS_004155"
     CDS             3072..4289
                     /gene="caiB"
                     /locus_tag="ACHOWS_004155"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFQ0049650.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4362..5915
                     /gene="caiC"
                     /locus_tag="ACHOWS_004156"
     CDS             4362..5915
                     /gene="caiC"
                     /locus_tag="ACHOWS_004156"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFQ0049651.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            6024..6809
                     /gene="caiD"
                     /locus_tag="ACHOWS_004157"
     CDS             6024..6809
                     /gene="caiD"
                     /locus_tag="ACHOWS_004157"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFQ0049652.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEAQRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6815..7405
                     /gene="caiE"
                     /locus_tag="ACHOWS_004158"
     CDS             6815..7405
                     /gene="caiE"
                     /locus_tag="ACHOWS_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFQ0049653.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAIIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYK
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7524..7919)
                     /gene="caiF"
                     /locus_tag="ACHOWS_004159"
     CDS             complement(7524..7919)
                     /gene="caiF"
                     /locus_tag="ACHOWS_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFQ0049654.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8180..11401)
                     /gene="carB"
                     /locus_tag="ACHOWS_004160"
     CDS             complement(8180..11401)
                     /gene="carB"
                     /locus_tag="ACHOWS_004160"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0049655.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11419..12567)
                     /gene="carA"
                     /locus_tag="ACHOWS_004161"
     CDS             complement(11419..12567)
                     /gene="carA"
                     /locus_tag="ACHOWS_004161"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFQ0049656.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2997 a         3306 c         3358 g         3071 t
ORIGIN      
        1 ttgaaagtca gatgctattt ttttactcac aacagaacat aacaaactga ttaataatca
       61 aaattaacga aaaaatgccc tctattcacc cgaaacaaaa atgtgataac aatcacagaa
      121 tgcagcttat tgaataccca ttatgagtta gccattaacg cgtccacgag gttaataata
      181 attatattaa atgttaacaa aaataaaaca aacgggaacg caaaataaat gttcgttttc
      241 acagtggaat taactcatga agaatgaaaa gagaaaaacg ggaatagaac cgaaggtttt
      301 ctttccgccg ttaataatcg tcggcatact ttgttggctt acagtcagag atctggatgc
      361 ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc aatgtatggg gatgggcatt
      421 tgaatggtat atggtggtga tgcttttcgg ctggttctgg ctggtgtttg gcccgtatgc
      481 caaaaagcgt ttaggtaacg aaccaccaga atttagtacc gccagttgga tctttatgat
      541 gttcgcctcc tgtacgtctg ctgccgtact gttctgggga tcgattgaga tctactacta
      601 catctccacc ccgccgtttg gcttagaacc gaactcaaca ggcgcgaaag agttggggct
      661 ggcttacagc ttgttccact ggggacctct gccgtgggcc acttacagct tcctttcagt
      721 cgccttcgct tacttcttct ttgtccgcaa aatggaagtg attcgcccca gctccaccct
      781 ggtgccgctg gtaggtgaaa aacacgccaa agggttgttc ggcactatcg tcgacaactt
      841 ctatctcgtc gccttgatct tcgcgatggg taccagtctg ggccttgcca cgccgctggt
      901 gaccgagtgt atgcaatggt tgtttggcat tccgcatacc ctgcaactgg acgctatcat
      961 cattacctgc tggattatcc tcaacgccat ttgcgtcgct tgcggtctgc aaaaaggggt
     1021 acgtatcgcc agtgacgtgc gtagttacct gagcttcctg atgctgggtt gggtgttcat
     1081 cgtcagcggt gccagcttca tcatgaacta cttcaccgat tcggtgggga tgttgctgat
     1141 gtatctgccg cgcatgttgt tctataccga tcccatcgct aaaggcggct tcccgcaggg
     1201 ctggaccgtg ttctactggg catggtgggt gatttacgct atccagatga gcatcttcct
     1261 cgcccgcatc tcccgtggtc gtaccgtgcg tgaactgtgc ttcggcatgg tgctggggct
     1321 gacagcgtca acctggatcc tgtggactgt actcggtagt aacactctgc tgttgataga
     1381 taaaaacatc atcaacattc caaatctgat cgaacagtac ggtgtggcgc gcgccatcat
     1441 cgaaacctgg gccgctctgc cactcagcac cgccaccatg tggggcttct tcatcctctg
     1501 ctttattgcc accgttacgc tggttaacgc ctgctcttat accctggcga tgtccacttg
     1561 ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg cgtatcggct ggtcaattct
     1621 ggttggcatt atcggtattg ttctgctggc gctcggcggc ctgaaaccga ttcaaaccgc
     1681 cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt atggtgacgc tctcctttat
     1741 taaagacgcg aaacagaact ggaaagatta attaaccccc aaaatatcaa gaggttgaaa
     1801 gatggatttt aatttaaatg atgagcagga actgtttgtc gccggtatcc gcgaactgat
     1861 ggccagcgaa aactgggagg cctattttgc cgagtgcgac cgtgacagcg tctacccgga
     1921 acgttttgtc aaagcactgg cggatatggg tatcgacagt ctgctgattc ctgaagagca
     1981 cggtggtctt gacgcgggtt ttgttactct cgccgccgtg tggatggagc tgggacgtct
     2041 gggggcacca acctatgtgc tgtaccagtt gccgggcggg ttcaatacct tcctgcgcga
     2101 aggcacacaa gagcagatcg acaagattat ggctttccgc ggcaccggta agcagatgtg
     2161 gaactcagcg attaccgaac cgggcgcggg ctccgacgtg ggtagcctga aaacgactta
     2221 tacccgtaga aatggtaaga tttatcttaa tggtagtaag tgttttatta ccagcagcgc
     2281 ctacaccccg tacatcgtgg tgatggcgcg cgacggggct tctccggaca aacctgtcta
     2341 caccgaatgg tttgttgata tgagcaaacc gggcatcaaa gtgaccaaac ttgagaagct
     2401 cggtctgcgt atggatagct gctgtgaaat cacttttgac gacgtggaac tggacgagaa
     2461 agacatgttc ggtcgggaag gtaacggatt taaccgcgtc aaagaagagt tcgaccatga
     2521 acgtttcctg gtagccctga ccaactacgg tacggcgatg tgcgcctttg aagatgcggc
     2581 gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt ggtcgtttcc agttgattca
     2641 ggaaaaattc gcccacatgg cgatcaaatt aaactccatg aaaaacatgc tgtatgaagc
     2701 agcgtggaaa gcagacaacg gcaccatcac ctctggcgat gcagcgatgt gcaaatactt
     2761 ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg caggtgctgg gcggtgtcgg
     2821 gattgcgggc aaccaccgca tcagccgctt ctggcgtgac ctgcgtgtag accgcgtttc
     2881 cggaggatct gacgaaatgc agatcctgac gctgggtcgc gcggtgctga agcaataccg
     2941 ttaagtccgt gcgctgcctg atgcgacgct gacgcgtctt atcaggccta cgggagcacg
     3001 aagatggccg gataaggcgt ttacgccgca tccggcagtc atgcgctaca taaatttttc
     3061 aggagctaat aatggatcat ctacccatgc cgaaattcgg gccgctggcc ggattacgcg
     3121 ttgtcttctc cggtatcgaa atcgccgggc cgttcgccgg acaaatgttc gcagaatggg
     3181 gggcggaagt tatctggatc gagaacgtcg cctgggccga caccattcgc gttcaaccga
     3241 actacccgca gctctcccgc cgcaatttgc atgcgctgtc gttaaatatt ttcaaagatg
     3301 aagggcgcga agcgtttctg aaattaatgg aaaccaccga tatcttcatc gaagccagta
     3361 aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt cctgtggcag cacaacccaa
     3421 aactggttat cgctcacctt tccggttttg gtcagtatgg caccgaggag tacaccaatc
     3481 ttccggccta taacactatc gcccaggcct tcagtggtta cctgattcag aacggtgatg
     3541 ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta cttttctggc ctgaccgcca
     3601 ccacggcggc gcttgcagca ctgcataaag tgcgtgaaac cggcaaaggc gaaagcatcg
     3661 acatcgccat gtatgaagtg atgctgcgta tgggccagta cttcatgatg gattacttca
     3721 acggcggcga aatgtgcccg cgcatgacca aaggtaaaga tccctactac gccggttgcg
     3781 gtctgtataa atgcgccgac ggctacatcg tgatggaact ggtgggcatt actcaaattg
     3841 cagagtgctt taaagatatt ggcctcgcac acctgctcgg tacgccggaa atcccggaag
     3901 gcactcagct tatccaccgt atcgaatgcc cttacggccc actggtagaa gagaaactcg
     3961 atgcctggct ggcggcacat accatcgcag aagttaaaga acgctttgcc gaactgaata
     4021 tcgcctgtgc caaagtactg accgtaccgg aactggaaag caatccacag tatgtggctc
     4081 gcgaatcaat cactcagtgg caaacgatgg atggtcgcac ctgcaaaggg ccgaatatca
     4141 tgccgaaatt caaaaataac cccggacaaa tctggcgcgg aatgccctca catggcatgg
     4201 acacggctgc tattttgaaa aatatcggct acagcgaaaa cgacattcag gagttggtca
     4261 gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt cagtcagctt gcctgactag
     4321 cgcccgctca ataacaaacg aacaaaatgg atagaggtgc aatggatatc attggcggac
     4381 aacatctacg tcaaatgtgg gacgatctgg cggacgttta cggtcataaa acggcgctga
     4441 tttgtgaatc cagcagcgga gtcgttaacc ggtatagtta tcttgagtta aatcaggaga
     4501 ttaaccgcac ggcaaacctg ttttatgtgc tggggattcg caaaggcgac aaggttgctc
     4561 tacatctcga caactgcccg gaatttatct tttgctggtt cgggttggca aaaattggcg
     4621 cgattatggt gccgattaac gcccgcctgt tgcgcgaaga aagcacgtgg atcctgcaaa
     4681 atagccaggc gtgcctgctg gtgaccagtg cgcaatttta tcctatgtat caacagattc
     4741 agcaggaaga tgcctctcag ttgcggcaca tttgcctgac agatatggta cttcccgctg
     4801 atgatggcgt gagttcgttt actcaacgga aaaatcaaca acctgccacc ttgtgctatg
     4861 caccgccgct atcgactgac gatacggcgg aaattctctt cacctccggc accacctccc
     4921 gaccgaaagg ggtggtgata actcattaca acctgcgctt cgctggatat tactccgcct
     4981 ggcagtgtgc actgcgtgac gatgacgtct acctgacggt aatgcctgcg tttcatatcg
     5041 attgccagtg tactgcggcg atggcggcgt tttctgccgg ggccaccttt gtgctggtcg
     5101 agaaatacag cgcccgcgcc ttctggggac aggtgcagaa gtaccgcgcc accattaccg
     5161 aatgtattcc gatgatgatt cgtacgttga tggtgcagcc gccttcagcc aacgatcggc
     5221 aacactgcct gcgggaagtg atgttttatc tcaacctgtc ggagcaggaa aaagacgcgt
     5281 tttgtgaacg cttcagcgtt cgcttgctga cgtcttatgg gatgacggaa accattgtgg
     5341 gcattatcgg cgatcgccct ggcgataaac ggcgctggcc gtcgattggt cgggcgggct
     5401 tttgctacga agcggagatc cgcgacgatc acaatcgtcc cctcccggct ggtgagctcg
     5461 gtgaaatctg tattaaaggc gtaccaggga aaaccatctt caaagagtat tttctcaacc
     5521 cgaaagccac tgcgaaagtg ctggaagccg atggctggct acataccggc gataccggat
     5581 accgcgacga agagggcttt ttttatttcg tcgatcgccg ctgcaatatg atcaaacgcg
     5641 gtggcgagaa tgtctcctgc gtggagctcg aaaatatcat cgccacccat ccaaaaattc
     5701 aggatatcgt ggttgtgggt attaaagatt cgattcgcga tgaagccatc aaagcatttg
     5761 tggtgctgaa tgaaggtgaa acattgagcg aagaggaatt cttctgcttc tgtgaacaga
     5821 atatggcgaa atttaaagtg ccctcttatc tggagatcag aaaagatctg ccacgtaatt
     5881 gctcggggaa aataattaga aagaatctga aataaatgaa acggcaggga actaccctgt
     5941 cagctaataa tcacgctcta aaacaatatg cattttttgc agggatgttg tcgtccctga
     6001 aaaagcaaaa atggagaaaa ggaatgagtg aatcattaca tctgacccgc aatggatcaa
     6061 ttctggaaat tacccttgat cgtccaaaag cgaatgctat tgatgcaaaa accagctttg
     6121 aaatgggcga agtatttcta aatttccgtg acgatccgca attacgtgtc gccattatta
     6181 ccggtgccgg agagaaattc ttttccgcgg gctgggattt aaaagcggca gcagaaggcg
     6241 aagcaccgga tgctgacttt ggcccgggag gttttgcggg attaaccgaa attttcaatc
     6301 tcgacaaacc ggttatcgca gctgtgaacg gctacgcctt tggcggcggt ttcgaactgg
     6361 cgctggcggc agattttatt gtttgtgccg ataatgccag cttcgccctg ccggaagcca
     6421 aactgggcat cgttcctgac agcggcggcg tgctgcgtct gccgaagatc ctgccgcctg
     6481 ccatcgtcaa tgaaatggtg atgaccggca gacgaatggg cgcagaagag gcgcagcgtt
     6541 gggggatagt caaccgcgtg gttagccagg cggaactgat ggataacgcc cgcgaactgg
     6601 cgcagcagct ggttaacagc gctccactgg cgattgcggc gctgaaagag atctaccgca
     6661 ccaccagcga aatgccggta gaagaagcgt atcgctatat tcgcagcggc gtgttgaaac
     6721 actatccatc ggttctgcat tcggaagatg ccattgaagg gccgctggcg tttgccgaga
     6781 agcgcgatcc ggtgtggaaa ggacgttaac aaccgtgagc tattacgcct ttgagggatt
     6841 aattccggtg gttcacccga cggcgtttgt ccatcccagt gcagtattga ttggcgatgt
     6901 gattgtggga gccggtgtct acatcggccc actcgcctca ctgcgtggtg actacgggcg
     6961 gttgatcgtg caagcgggag ccaatattca ggatggctgc attatgcatg gctactgcga
     7021 cactgacacc atcgttgggg aaaacggcca tatcgggcac ggagcgatcc tgcatggttg
     7081 tgtgattggt cgcgatgcac tggtcgggat gaacagcgtg attatggatg gcgcgatcat
     7141 tggcgaagag agcattgttg ccgctatgag ctttgtcaaa gcgggctttc gcggcgagaa
     7201 acgccagttg ttgatgggta cgccagcccg cgctgtacgc agcgtgagtg acgacgagtt
     7261 acactggaaa cggttgaata ccaaagagta taaggatctt gtcgggcgct gtcatgcatc
     7321 attacatgaa acgcagccac tgaggcaaat ggaggaaaat cgcccccgtt tgcaggggac
     7381 gacggatgtg acgccgaaac ggtgatcttt ccggatgcgg cgtaaacgcc ttatccgacc
     7441 tggctggaaa ggaaccgtag gccgtataag atgcgccagc atcgcatccg gcgaccaggt
     7501 cgcacggtat caacgaaacg acattaacga cgcatactct ttgacaacat catctgccac
     7561 ttctgctcgc ggttaagttc cgtgagcgga acggcaggaa tacgtggcgt ctttcttacg
     7621 ccgactaatt tttcccgact gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg
     7681 accactttaa ccagtcgctg acactggcat ccccgccctt ccagcttatt agggatcatc
     7741 ttaacttcgc agcttatttc tgtgacttcc gacagaatat aagtcagggt attaaccgcc
     7801 ttgctgtgtt caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca
     7861 gccatcatcc attcggcgat taacaagtag agtggttttt caacatatcc ttcacacata
     7921 cgaactccag tttcacaaat taaaagtctg gctatgactc gttttgcagc aattaaacaa
     7981 ggccatatta aaattgtgga tatatcgata gtatgcaact tcaccgtcga taagtgtgag
     8041 ctaaatcctg cttatgtcat caaaaatatc gattccccaa aagatttact taatgggcaa
     8101 aaaaaacggt cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa
     8161 tatccgccat gacacgctat tatttgatct gcgcgtgcat ttcctgcacc gaaattactt
     8221 tttcagtcgc atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt
     8281 cgtaatgcac tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac
     8341 gacgacctga ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa
     8401 tgtgcggacg accttcatgc accttgttca ccagacgcgg attaatgcct gcttcgccca
     8461 gcacaatcgc cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg
     8521 ccaggtccac cacgcgttct ttatcgcctt cgcgcacgga aagcaacgca cgaccgtgtt
     8581 tcttcatggt ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc
     8641 ccacgcccat gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcccg
     8701 ggaatttatt gaacggcagc accacttctt tcaccgagta gtacggcggg ataacttctt
     8761 tggttacgcc ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca
     8821 gcggtacgcc ggtggctttg gagacgaacg gaacggtacg cgccgcacgc gggttaactt
     8881 caatcaggta gacttcgttg tttttcaccg caaactgcac gttcatcagg ccgcgcacct
     8941 gcaattcgaa ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta
     9001 aagtgtaggc tggtagcgaa catgcagagt caccggagtg cacgcccgcc tgctcgatgt
     9061 gctccatgat gccgccaatc agcaccattt caccgtcgca gatggcatcc acgtcaactt
     9121 ctactgcgtc atcaaggaag tggtccagca acactggcgc atcgttagat acgctgaccg
     9181 ctgtctggaa gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc
     9241 cgagaacgta agacggacgc accaccagcg ggtagccaat ctctttcgct ttctcaaccg
     9301 ccatttcgat ggtggtaacg gtggcgttcg ccggttgttt cagtttcaga cgctcaaccg
     9361 catgctggaa gcgttcacgg tcttccgcac ggtcgatggc atccgggctg gtaccgataa
     9421 ccggtacgcc agcagcttcc agcgcgcgcg ccagtttcag cggggtctga ccgccgtact
     9481 ggacgataac gcctttcggc ttctcaatac gcacaatttc cagcacgtct tccagagtta
     9541 ccggctcgaa gtagagacgg tcagaggtgt cgtagtcggt ggagacggtt tccgggttac
     9601 agttaaccat aatggtttca taaccatctt cgcgcagcgc cagcgaggcg tgtacacagc
     9661 agtagtcgaa ttcgataccc tgaccgatac ggttcgggcc gccgccgagc accatgattt
     9721 tttcacggtc ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag
     9781 cggtgtcggt ggcgaactct gccgcacagg tatccacgcg cttataaacc gggtgcaagt
     9841 catactggtc gcgcagcttg cggatttctg cttcacgcac gcccgcgagt tttgccagac
     9901 gcgcgtcggc aaagcctttg cgtttcagct ggcgcaggaa gtcagcgttc aggccagtga
     9961 tgcccacttc tgcgactttc tcttccagac gcaccagctc ttcaatttgt accaggaacc
    10021 agcggtcaat gttggtcagg ttgaacacgc cgtctacgga caggcccgcg cggaaagcat
    10081 cggcgatata ccagatacgc tctgcgcctg catctttcag ttcgcggcga attttggtca
    10141 gcgcttccgg atcatcgagg ctgactttcg ggtcgaagcc agttgcgccc acttccaggc
    10201 cgcgcagcgc tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa
    10261 ccgatttcat ctgagtggtc agacggtcgt tcgccccggc gaatttctcg aagttgaagc
    10321 gaggaatttt agtcaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgcccac
    10381 cggtgatgtc gttcatcagc tcgtcgaggg tgtaacccac cgccagtttc gccgccactt
    10441 tggcaatcgg gaaaccagtg gctttcgacg ccagcgcaga ggagcgagac acgcgcgggt
    10501 tcatttcgat aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acgttggaac
    10561 caccggtttc aacgccgatt tcacgcagta ccgccatcga ggcgttacgc atgatttgat
    10621 attctttgtc ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc
    10681 ccatcgcatc aaagttttcg atagagcaga caatgatgca gttgtcgttt ttatcacgca
    10741 ccacttccat ctcgtactct ttccagccga tcagcgactc atcaatcagc agctctttag
    10801 tcggagagag atccagacca cgggcgcaaa tttcttcaaa ctcttcgcgg ttataagcga
    10861 taccgccgcc gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagccaactt
    10921 cagcggcaac cgccagcgct tcttccattg aatgagcgat accggaacgc gcggtttcca
    10981 gaccgatttt cttcatcgct acgtcgaaac ggcggcggtc ttcggcttta tcaatcgcat
    11041 cggcagtggc accaatcatg gtgacgccga actcttccag cacgccctga cgctcaagct
    11101 caagcgcaca gttcagcgcc gtctgccccc ccatggttgg cagcaccgcg tccggacgct
    11161 ctttttcaat aatcttgcgt accacttccc agtgaatcgg ttcgatgtag gttgcatcag
    11221 ccatttccgg gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac
    11281 cctcttcacg cagggcttta cacgcttgcg cgccagagta gtcaaactca cacgcctgac
    11341 cgataacaat cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca
    11401 tggctctttt actcctgatt acttagcggt tttacggtac tgctcaatta actcgataaa
    11461 gtggtcaaac aacggcgcgg cgtcgtgtgg accagggctg gcttccgggt gtccctggaa
    11521 gctgaacgcc ggtttatcgg tacgatgaat gccctgtaac gtaccgtcga acagggattt
    11581 atgtgtgaca cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg
    11641 ggcggtaatc atcaccacat ttttctcaac atctttaacc ggatggttgc cgccgtggtg
    11701 accaaatttc atttttacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag
    11761 gcagatgccg aataccggaa tatcggtttc gaggaatttc tggatggcgg taatggcgta
    11821 atcgcacggg gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt
    11881 cagcacgtct tccgcagaag tttgcgccgg aacgatggtc agacgacagc ctctatccac
    11941 caacatgcgc aggatgttgc gcttggcacc aaaatcataa gccacaacat ggaacggcag
    12001 ctcgtcttct ttcttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca
    12061 gctataggct tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa
    12121 cgcgcgggct ttttctaatg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc
    12181 attctgtgcg cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat
    12241 cgccacgatg ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct
    12301 ggcaatcagc ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc
    12361 atcggcgtca ttagtgccga cattaccaat atggggataa gtaagagtaa cgatttgacg
    12421 agaataggaa ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac
    12481 ttccccaacc gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc
    12541 cagaaccaat agcgctgact taatcaaaac accctccaga gaatattcac tcactttatt
    12601 tgcatattaa ttcacaatga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct
    12661 ggcaaacggc ggcattctgg agatatgggc gctaaaagtc aacttaatgg cgggattttt
    12721 tatattattt tg
//
LOCUS       DAZKJR010000142        12647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKJR010000142 DAZKJR010000000
VERSION     DAZKJR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.143"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..474
                     /locus_tag="ACHOWS_004162"
     CDS             <1..474
                     /locus_tag="ACHOWS_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFQ0049657.1"
                     /translation="APAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAIMVDGKPVI
                     PGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQKAGIASFCPYNIGPGKCFPST
                     FYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            772..1239
                     /locus_tag="ACHOWS_004163"
     CDS             772..1239
                     /locus_tag="ACHOWS_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFQ0049658.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     EQANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATSTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAKEYIRTQCIN"
     gene            1263..1487
                     /locus_tag="ACHOWS_004164"
     CDS             1263..1487
                     /locus_tag="ACHOWS_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049659.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1935..2483
                     /locus_tag="ACHOWS_004165"
     CDS             1935..2483
                     /locus_tag="ACHOWS_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFQ0049660.1"
                     /translation="MNVNKKKLAEIFNVDIRTIDRWQTQGLPQISGGGKGVEVIFDSA
                     KAIEWYAQREADIENEKLRKEVEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     REEGLVLETELFTYIFQRVAQNISGILVRVPQTLQRKYPEISPAHLDAMKTEIAKASD
                     VASEAGENVRRWIDDFRRTEGG"
     gene            2455..4383
                     /locus_tag="ACHOWS_004166"
     CDS             2455..4383
                     /locus_tag="ACHOWS_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFQ0049661.1"
                     /translation="MISDELRAANSAGAIATGLLALKIPVPLTTVQWADRHYYLPKES
                     SYTPGRWETLPFQVAIMNSMGNDRIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVSVLLDLAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            4367..4573
                     /locus_tag="ACHOWS_004167"
     CDS             4367..4573
                     /locus_tag="ACHOWS_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFQ0049662.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            4570..6162
                     /locus_tag="ACHOWS_004168"
     CDS             4570..6162
                     /locus_tag="ACHOWS_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0049663.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRSRFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETRPRET"
     gene            6152..7657
                     /locus_tag="ACHOWS_004169"
     CDS             6152..7657
                     /locus_tag="ACHOWS_004169"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFQ0049664.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALSAEVCQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            7694..8041
                     /locus_tag="ACHOWS_004170"
     CDS             7694..8041
                     /locus_tag="ACHOWS_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFQ0049665.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            8099..9127
                     /locus_tag="ACHOWS_004171"
     CDS             8099..9127
                     /locus_tag="ACHOWS_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFQ0049666.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            9179..9562
                     /gene="gpFI"
                     /locus_tag="ACHOWS_004172"
     CDS             9179..9562
                     /gene="gpFI"
                     /locus_tag="ACHOWS_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFQ0049667.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEPLNNTDAPDDVKAVRVRKCLHVMGYCPETGRPVELT
                     FRGMRVLVPSSLAMAMIQHGTAEYA"
     gene            9555..9908
                     /locus_tag="ACHOWS_004173"
     CDS             9555..9908
                     /locus_tag="ACHOWS_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFQ0049668.1"
                     /translation="MRDVQNAFDAALVGVDSTIVEVMGIRVQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            9924..10499
                     /locus_tag="ACHOWS_004174"
     CDS             9924..10499
                     /locus_tag="ACHOWS_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0049669.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTTINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            10496..10891
                     /locus_tag="ACHOWS_004175"
     CDS             10496..10891
                     /locus_tag="ACHOWS_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFQ0049670.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            10899..11648
                     /locus_tag="ACHOWS_004176"
     CDS             10899..11648
                     /locus_tag="ACHOWS_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0049671.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDAGWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            11665..12087
                     /locus_tag="ACHOWS_004177"
     CDS             11665..12087
                     /locus_tag="ACHOWS_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFQ0049672.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAEQQGTTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            12114..12527
                     /locus_tag="ACHOWS_004178"
     CDS             12114..12527
                     /locus_tag="ACHOWS_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFQ0049673.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPVCEEEQTEMPDEEDMLMQKAAGVAGGVRFCGVGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            12508..>12647
                     /locus_tag="ACHOWS_004179"
     CDS             12508..>12647
                     /locus_tag="ACHOWS_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0049674.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQG"
BASE COUNT         2975 a         2993 c         3869 g         2810 t
ORIGIN      
        1 gcgcctgcgc ctgaaatcct cgaccagttt ctggatgaaa aagaaggcaa ccacaccacg
       61 gcataccgtg atggcgcggg tatctggacc atctgccggg gtgccatcat ggtggatggt
      121 aagcctgtga ttcctggcat gaagctgtcg aaggggaaat gcgaccgggt taacgccatt
      181 gagcgtgata aggcgctggc atgggtggag aaaaacatca aagtgccact gaccgaaccc
      241 cagaaagcgg ggatcgcgtc attctgtcct tacaacatcg gcccaggtaa gtgttttccg
      301 tcgacgtttt ataaacgaat taatgcaggc gatcgcaggg gagcgtgtga agcgattcgc
      361 tggtggatta aggacggtgg cagagactgc cgtatccgct caaataattg ctacggtcag
      421 gtatcccgtc gcgaccagga gagtgcgctg gcatgctggg gtatcgacag gtagtagaat
      481 attttgctga aaaatgacgt tgaccaacgc ggacggataa cacgaaatcc tgcgaactgg
      541 caaaacctaa gtgaataaaa gtaaaaaccc cgtttgttgg cagcaagcgg ggttttgtgt
      601 ttcctgactc cggaaaagtc aaaggagaaa gtgtgtttga ttttagcaaa ctgattcggg
      661 agattcgagt gatggctgaa aaattatcca cctggaagtt catccttatc tggctggtgt
      721 ttgtgattat ggcctccggt tatttcatcg gtcagatacg ctggtggtga aatgaaccgc
      781 gttctgtgtg tggtcatcat tgccttgctg gtggcctgtg gtgcgcttag tctggggctg
      841 aatcattacc gcgataacgc catcacctac aaagcgcagc gcgataaaaa agccagagag
      901 ctggaacagg cgaatgccac cattactgac atgcaggtgc gccagcgtga tgttgctgcg
      961 ctcgatgcaa aatactcgag ggaattagcc gatgcgagag ctgaaaatga aactctgcgt
     1021 gctgatgttg ccgctggtcg taagcgcctg cggatcaacg ccacctgcca cggtaccgtg
     1081 cgtgaagcca ccagcacctc cggcgtggat aatgcaaccg gcccccgact ggcagacacc
     1141 gctgaacggg attatttcat cctcagagaa cggttgatga caatgcagaa gcagctggaa
     1201 ggggcgaagg aatatatccg cactcagtgt attaactagt atttttgtta tccggagaat
     1261 gcatgaagaa attactggta accgtagaac cttttcaggg aacaattccg ttccgtattt
     1321 tgcagcgtgg tcgtgttctt gttgaaggtt cgttcagtgg taaatgtacg caattacact
     1381 cccggacctt tcaggtgaat gccacgaatg aagagctaac cgttgagtgt acgatgaatg
     1441 ccgctaaatg ccgcatggta tccgctgcat tacagccagt gtgttgagcg accttattat
     1501 ccatgcgcgg tattgtcgcc gtattcccgt attaacagag accgcagccc gacagggaga
     1561 ctcctctgcg cgagtgtgcg gggataatca aaaacgatac acaccggggt ttaccgcgtt
     1621 aacggagcgc ggcgttgtcc cctcatggtc gctggtccgg tgcgatggtg gaagaaactg
     1681 gattttgttg caactgataa ccattatcat tttcgcgggt cctttccggc gatccgggcc
     1741 gttacggggc ggcgacctcg cgggttttca ctatttatga aaattttccg ggaaaaagca
     1801 tgtcagttct tcttgctgtt aacatattgt ttttaaagaa ataatcatca agaaagaaac
     1861 gacatcagaa cgctgaaaaa tctgtctttt ttggcatttc atgtcgtaac tgatttttga
     1921 taaggaattg atgaatgaat gttaataaaa agaagcttgc tgaaattttt aatgtcgata
     1981 tacgcactat tgatcgctgg caaactcagg ggctccctca gatatctggt ggaggaaaag
     2041 gtgttgaggt catctttgat tctgcgaagg cgatcgaatg gtatgctcag cgcgaagctg
     2101 atattgaaaa tgaaaaactc cgtaaagagg tcgaggattt gagggctgcc agcgaatcag
     2161 accttcagcc cggcaccatt gattacgaac gttaccgact gacgaaggca caggcagatg
     2221 cacaggagct gaaaaatgct cgtgaggaag gccttgtcct cgagacggag ttatttacct
     2281 acatctttca gcgagtggca cagaatatat ctgggatcct tgtccgtgtc cctcagacac
     2341 tgcagcgtaa ataccctgag atatcacccg cacatcttga tgctatgaaa actgaaatcg
     2401 cgaaagcatc cgatgtggct tctgaagccg gtgagaatgt gcgcaggtgg attgatgatt
     2461 tcagacgaac tgagggcggc taattctgca ggagcgatag caaccggcct tcttgcgcta
     2521 aaaattcctg tccccctgac gacagttcag tgggcagatc gacactatta ccttccgaaa
     2581 gagtcatctt acaccccggg gcggtgggaa acactgccat ttcaggttgc catcatgaac
     2641 agcatgggga atgaccggat ccgcacggtt aatctgatta aatctgcccg tgttggttat
     2701 acaaagatgt tgctgggagt ggaggcttat tttattgagc ataaatcacg caacagcctt
     2761 cttttccagc ctacggattc tgctgctgaa gattttatga aatctcatgt ggaacccacg
     2821 atcagggatg tgtcggtttt actcgatctt gcaccgtggt ttgggcgtaa acatcgtgat
     2881 aataccctca cgctgaaacg cttttcatcg ggtgtgggtt tctggtgcct gggtggtgcg
     2941 gcagcaaaaa actaccgtga aaaatccgtg gatgtggtct gctatgacga actttcctcg
     3001 ttcgagccgg atgttgaaaa agagggctcg ccaactctgc tgggggataa gcgtattgag
     3061 ggctcggtat ggccaaaatc cattcgcggc tcgacgccta aaataaaagg cacctgccag
     3121 atcgaaaaag cggccaacga gtcggcgcat ttcatgcgtt tttatgtgcc ctgcccgcac
     3181 tgtggggagg agcagtatct gaaatttggc gatgaatcca cgccttttgg gcttaaatgg
     3241 gagaaggaca gtcccgaaag tgttttctac ctctgtgaac atcatggctg cgtgatccat
     3301 cagtctgagc ttgaccagag caacgggcgg tggatctgtg aaaacacggg gatgtggacc
     3361 cgtgacggtc tgacgttttt cagcgctgcg ggtaatgaaa ttccgccgcc gcgctccatc
     3421 acattccata tctggacggc gtacagtccg ttcaccacct gggtacagat tgtctatgac
     3481 tggctggatg cactgaaaga tcccaacggc ctgaaaactt ttgtgaacac cacgctgggc
     3541 gagacctggg aagaggccgt gggcgaaaaa ctcgatcacc aggtgctgat ggataaggtt
     3601 gtgcgttaca cggcggcggt acctgcccgg gtggtttatc tgacggcggg cattgactcg
     3661 cagcgtaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
     3721 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agactctgtt acgtgtggat
     3781 gcggcgatca acaaaaaata ccgccatgca gacggaaccg aaatgaccat ttcccgtgtc
     3841 tgctgggaca ccggggggat cgatggcgaa atcgtttatc agaggtcaaa aaaacacggt
     3901 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
     3961 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tggggacgga caccgcaaaa
     4021 gaaattctct atgcccgtat gaaagccgat cccacgcctg cggatgaagc cacgtcgtat
     4081 gccatccgtt ttcctgatga tccggagatt ttttcgcaga cagaggcgca gcaactggtc
     4141 gcggaagagc ttgtggagaa gtgggaaaaa ggaaagatgc gtctgctgtg ggataacaaa
     4201 aagcggcgta acgaagcgct ggactgcctg gtgtatgcct acgcggcatt acgtgtgtcc
     4261 gtgcaacgct ggcagcttga tctggctgta ctggcaaaat cccgggaaga agagacgacc
     4321 cggccaaccc tgaaagaact ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc
     4381 tgaactgcag gcgctgcgtc aggcgcgcct tgatttatta accggtaaac gggtggtgtc
     4441 tgtccagaaa gatggtcgca gaattgaata tacggcggct tctctggatg agcttaaccg
     4501 tgcgatcaat gatgcggagt cggtactggg gacaacccga cgtcgccgtc gtccgctggg
     4561 agtgaggtta tgaaacgaac gcctgtcctg attgatgtga acggcgttcc gcttcgggag
     4621 agcctcagct accacggggg cggtgcagga tttggtgggc aaatggcaga gtggttgcca
     4681 ccctcgcaga gtgccgatgc ggccctgctg cccgcgttgc gtctggggaa tgcccgtgca
     4741 gatgatctgg tacgcaataa cgggatagcg gctaatgcgg tggccctgca taaggatcat
     4801 attgtcgggc atatgtttct gatcagctac cgtccgaact ggcgctggct ggggatgcgg
     4861 gagactgcgg caaaaagctt tgtcgatgag gtggaggcgg cctggtcgga atacgccgaa
     4921 gggatgtttg gcgagatcga cgtggaagga aaacgcacgt tcacggaatt tatccgtgaa
     4981 ggcgtgggcg ttcatgcgtt taacggcgaa atctttgtgc agccggtctg ggatacggaa
     5041 accacgcagt tattccgcac gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca
     5101 ggacacggta tcgggaatcg ttttctgcgg gccggggtgg aggtcgatcg atatgggcgt
     5161 gccgttgcgt accatatctg tgaggatgat tttcctcgct ccgggagtgg acgatgggaa
     5221 cggatcccgc gtgaacttcc caccgggcgt ccggccatgc tgcatatttt cgagccggtg
     5281 gaggacgggc agacccgtgg ggccaatcag ttttacagcg tcatggaacg gctgaagatg
     5341 ctcgattccc tgcaggcaac acagcttcag tcagccattg ttaaagccat gtatgcagcg
     5401 acgattgaaa gtgaacttga taccgaaaag gcctttgaat atatcgcggg ggcaccgcag
     5461 ggtcagcagg ataatccgct cattaatatt ctggagaagt tctccagttg gtatgacacg
     5521 aatcacgtga cgctgggcgg tgtcaaaatt ccgcaccttt tccccggtga tgatctgaaa
     5581 ctgcagaccg cgcaggattc agacaatgga ttttctgcgc ttgaacaggc gctgctgcgg
     5641 tatatcgccg ccggtcttgg cgtttcctac gaacagttgt cccgtgatta ctcgaaggtc
     5701 agttactcaa gtgcccgcgc ctccgccaat gagtcgtggc gctattttat ggggcggcga
     5761 aaatttattg cggcccggct ggccacgcag atgttttcct gctggctgga agaggcactt
     5821 cttcggggga ttatccgtcc gccacggtca cgttttgatt tttatcaggc gcgatcagcc
     5881 tggtcacggg cagagtggat tggtgccgga agaatggcca ttgacgggct caaggaggtc
     5941 caggaatcag tgatgcgcat tgaggccgga ctgagcacgt atgagaaaga gctggcgctg
     6001 atgggtgagg attatcagga cattttccgc cagcaggtca gggaatccgc agagcgggaa
     6061 aaagccggac tctcacgtcc ggtgtggata gcacaggcgt atcagcagca gatagcggag
     6121 agccgcaggc cggaagagga gacaagacca cgtgagacgt aatctttcac acattattgc
     6181 cgcagcattc aatgaaccgc tgcttctgga gcccgcctat gcgcgggttt tcttttgcgc
     6241 gctcgggcgc gagatggggg cagcaagtct ttcggtacca caacagcagg tgcagcttga
     6301 tgctcccgga atgctggctg aaacggacga gtacatggcc ggaggtaaac gaccggcccg
     6361 tgtttaccgg gtggtgaacg gtattgctgt actgccggtg accggcacgc tggtgcaccg
     6421 gctgggtggc atgcggccat tttccggaat gacaggctat gacggcattg ttgcctgtct
     6481 tcagcaggca atggcggaca cctctgtccg gggcgtactg ctggatattg acagtccggg
     6541 cgggcaggcc gccggtgcgt ttgactgcgc tgacatgatt taccgcctcc ggcagcagaa
     6601 gccggtctgg gcactgtgta atgacacggc ctgttctgcg gccatgctgc tggcgtcggc
     6661 ctgctcccga cggctggtta cccagacatc ccgtatcggc tccattggcg tgatgatggg
     6721 ccatgtcagc tatgccggtc atctggcgca ggccggggtg gatatcacgc tgatttatgc
     6781 cggggcgcat aaggtggatg gcaatcagtt tgaagcctta tcggcagagg tgtgtcagga
     6841 catgcagcag cgcattgatg cggcgcaccg gatgtttgcc gaaaaagtgg cgatgtatac
     6901 ggggctgtct gtggaagctg tcacggggac agaggctgcc gtttttgaag gtcagtccgg
     6961 tattgaggcc gggctggcgg atgagttaat caatgcgtcg gatgccatca gcgtgatggc
     7021 tgcggcgctg aacacacatg atacaggagg cactatgccg caattaactg caacggaagc
     7081 cgccgcgcag gagaaccagc gagtgatggg gatcctgacg tgtcaggaag cgaaaggacg
     7141 tgaacagctt gccacgatgc tggcaggaca acagggcatg agcgttgaac aggcccgggc
     7201 gattctggct gcggcagcac cacagcagcc ggtggcatcc gcgcagagtg aagccgatcg
     7261 cattatggcg tgtgaagaag ctaaaggtcg tgaacaactg gcggcaacgc tggcggcgat
     7321 gccggagatg acggtggaaa aagcccgccc gatcctggcg gcttcaccgc aggcggatgc
     7381 cggaccctca ctccgtgatc agattatggc tttggatgag gcaaaagggg ctgaggcgca
     7441 ggctgaaaaa ctggcggcgt ttcccggaat gacggtggag gctgcccgcg acattctgtc
     7501 ctcatcgccg gataaagcag aaccggtttc tgcatccaca accgccatgt ttgaacgcat
     7561 catggcgaac cattcaccgg cagccgtgca gggtggcgtg tcacagacgt cggcagacgg
     7621 tgatgcggac gtgaaaatgc tcatggccat gccatgaagt cagtgctgac catcaatatg
     7681 aggtttttac aatatggtaa cgaaaaccat cactgaacaa cgtgcagaag tacgtatttt
     7741 tgccggtaat gatccggctc ataccgccac aggcagcagc gggatttctc aggcaacacc
     7801 ggcactgacg cccctgatgc tggatgaagc cagcgggaaa ctggtggtct gggacggaca
     7861 gaaagccggt agtgcggctg gcatactggt actgccgctt gaaggcacag agacggtgct
     7921 gacgtattac aagtcgggaa cctttgcgac ggaggcaatc cgctggcctg aaagtgtgga
     7981 tgaacacaaa aaggccaacg cctttgtcgg cacagccctg agtcacgcgg cgctgccgta
     8041 acacgttatc aggccaccgc gttggcctga ctgatttctg aatgaaagga actgatttat
     8101 gggattgttt acgacccgcc agttactcgg ttataccgaa caaaaagtga aatttcgtgc
     8161 gctgtttctg gaactgtttt tccgccgtac ggtgaatttc cacaccgaag aggtgatgct
     8221 ggacaaaatt accggaaaaa cgccggtggc agcctatgtc tccccggttg ttgaaggaaa
     8281 agtgctgcgt catcgtggtg gtgaaacccg cgtgttacgt ccgggctacg tcaagccgaa
     8341 acacgaattt aattaccagc aggcggtgga gcgccttccc ggtgaagatc cggcgcagct
     8401 gaacgacccg acctaccgtc gtctgcgtat catcaccgat aacctcaaac aggaagagca
     8461 cgccattgtc caggtggaag aaatgcaggc ggtgaatgcc gtgctgtatg gcaaatacac
     8521 catggaaggg gagcagtttg atactgtcga ggtggatttc gggcgctctg aaggaaataa
     8581 cattgagcag gctgacggta aaaaatggtc tgagcaggac cgtgatacgt ttgatccgac
     8641 gcatgatatt gacctctact gcgatcaggc cagcggcctt gtgaatatcg ccattatgga
     8701 cggtacggtc tggcgtctgc tgaatggctt taagctgttc cgcgaaaaac tggatacccg
     8761 tcgcggctca aattcacaac tcgaaacggc agtgaaagat ctgggcgcag tggtgtcctt
     8821 caaggggtat tacggcgatc tggccattgt ggtggcgaaa acgtcttatg tggcagagga
     8881 cggtaccgaa aaacgttatc tgccggaggg cacgctggtc ctgggaaata cgtcagcgga
     8941 gggcatccgt tgctatggtg ccattcagga tgcgcaggcg ttgtccgaag gtgtggtggc
     9001 ctcttcccgt tatccgaaac actggatgac cgtgggcgat ccggcccgtg aattcaccat
     9061 gacgcagtcc gctccgctga tggtgctgcc ggatccggat gagtttgtgg tggtacaggt
     9121 gaaataatcc gggagcgggg gcgaaatgcc cccgtgtctt ttttcacagg gggctgagat
     9181 ggcaacaaaa gaagaaaatc tgaatcgtct tcgtcaactg gctggtctgc tggggcgcga
     9241 ggcggatatg tcggggagtg ctgcggatat tgctcagcgt gtgtctgagt gggaagagga
     9301 gcttgctgct tcccgggagg gcattatgca ctctgatgag agcgggtctg atcaaaatga
     9361 cacagacgat ggtgagccgt tgaacaacac ggatgctccg gatgatgtta aagccgtccg
     9421 tgtgcgcaag tgcctgcatg tgatggggta ttgcccggag acaggccgtc ctgttgagct
     9481 tacgttccgg gggatgcgtg ttctggtgcc atcatcactg gcaatggcca tgatacagca
     9541 cggaacggct gagtatgcgt gatgttcaga atgcctttga tgccgccctt gtgggggtgg
     9601 acagcacgat tgttgaagtg atggggatcc gtgtgcagtt tacctccgga gcacaacgtg
     9661 gcggtgaagt tcagggggtt tttgacgatc cggagtcgct ggggtttgcc ggtggcgggg
     9721 tccgtattga aggaagcagc ccgtcattat ttgtgcggac ggatacggtg cgtgccgtgc
     9781 ggcgtggtga cacgctgacc attaacggcg agatgttctg ggtggatcgt gtttctccgg
     9841 atgacggggg cagctgttat ctctggctca accgtgggca accacccgct gttaaccggc
     9901 gacgataaac gcagggtgaa attatggcga taaaagggct tgatcaggcg attgataatc
     9961 tgagccgggt tcgtaaaaac gccattccgg cggcttcagc aatgacgatt aaccgcgtgg
    10021 ccacaacgac gattaatcag tcttcatcac aggttgcccg ggagacaaag gtacgccgga
    10081 aactggttaa ggaacggtcc agactgaaac gggccacggt cagaaatccg aatgccagaa
    10141 ttatcgttaa ccgcggtgat ctccctgtta ttaagctggg gatcagaatg ctggggcgtc
    10201 gtccggacag catactcaaa gccggtcagc atcgttatca gcgggcattt atccagcgat
    10261 taaataatgg gcgctggcat gttatgcaac gtcttcccca ggccagatat gagaagggca
    10321 atgacgacaa gggcaggaaa aagcgtaatc gccttcccat tcaggtggtg aaaatcccga
    10381 tggcggcccc actgaaacag gcgtttgatg aaaacgttga ccgtatccgt cgtgaacgcc
    10441 tgcccaaaga actggcatat gcgctgaaac aacaactgag gattgcgata aaacgatgaa
    10501 acatactgat atccgtgcag ccgtactgga tgcactggag aagcatgaca ccggggcgac
    10561 gctgtttgat ggtcgccccg ctgtttttga ggaggcggat tttccggcgg tcgcggttta
    10621 tctgacggat gcagagtata ccggtgaaga gctggatgct gatacctggc gggccacact
    10681 gcatattgag gtgtttttac cggcacaggt accggattca gagcttgatc agtggatgga
    10741 aagccggatt tacccggcga tggcggcgat ccctgcactg gcaggcatga ttaccacgat
    10801 ggttacgcag ggctatgagt atcgtcgtga tgacgatatg gcattgtgga gctctgcaga
    10861 tctgacttat tccattacat acgagatgtg aggatgatat ggcaacacca aatcccctgg
    10921 cgccggtaaa aggttccggc accacgctct gggtttacac cggtaagggc gatgcttatg
    10981 caaacccgct gtcagatgat gagtggacgc gtctggcaaa aataaaggat ctgacccccg
    11041 gcgagatgac ggcggaatcc tacgacgata actatctgga tgatgaggat gccggctggg
    11101 tatccaccgg gcaggggcag aaatccgccg gtgacaccag ttttacgctg gcctggaagc
    11161 ccggcgagaa agggcagcgt gatttgattg cctggtttga cagcagtgag acccgggcct
    11221 acaaaatccg cttcccgaac ggtacggtgg atgtgttccg tggctgggtg agcgccattg
    11281 gtaaagcggt gaccgccaaa gaagtgatta cccgtacggt aaaaatcacc aatatcggtc
    11341 gtccttcgct ggcggaggat cggggagaaa tcacaccggt caccggtatt accgtgacgc
    11401 cactaacggg gaatgtggca aaaggtcaga gcaccaccct gacagtggcc gttcagccgg
    11461 aaggcgcaac ggataaaaca ttccgcgcaa cgtcggcaaa tcagaatttc gccaccatta
    11521 ccgtgaaagg gaacacgatc accgtgaaag gtgttgcggc aggtaaagcg cagatccctg
    11581 ttgtcaccgg caacggtgag tttgcggcgg tggcggagat caccgtcacg gatggcgctg
    11641 cgggctgagg ggaggagatc aagcatgttc ctgaaaacag aacaatttga atataacggt
    11701 gtgtccgtca cgctgtcgga gctgtccgcg ctgcagcggt ttgattatat gaagtttgtt
    11761 tcagacgcag aacaacaggg gacaacgaag catgatgtcg tgcacattaa ccagcgatat
    11821 ctggaaacgg catccctgct tgtggcgatg tcgctatggc attcccattc cctcaaaggc
    11881 actctggcct ctccggagac agagatgcag cagatccgcc gtgaagtgat gctgggatgg
    11941 cctgctgatg cactgaatca ggcaacgaac cgggtgcttt atctttcagg tatgctggat
    12001 aaccggcacg atgccgatcc tgaacaaacc gggaaagcag aagcgactga gccggtaaca
    12061 tcaaaaaagc attcgaaggc gagctgaact ttgtcctgaa actggcgcga gagatgggga
    12121 gacccgactg gcgcgccatg cttgccggga tgacatccac cgaatatgcc gactggcgac
    12181 gtttttactg cacgcattat tttcaggata cccaactgga cgctcatttt tccgggctga
    12241 tgtacgccgt actcagcctg ttttttggcg atccggatat gcatccggcg gatttcagtc
    12301 tgcttgcgcc agtgtgtgag gaagagcaga cggagatgcc ggacgaggaa gacatgctga
    12361 tgcagaaagc ggcaggagtt gccggaggcg tccggttctg tggggtgggt gggggcgata
    12421 tttcaccttc tgcggatgtg gtggatgtca gcgaagatga tgttgcatta atgatggctt
    12481 cagcggggat ttccggaggt gtgagatatg tcccagccag cgggtgatct tgttattgat
    12541 ttgagtctgg atgcggcccg gtttgatgaa cagatggccc gggtacgccg ccatttttcc
    12601 agtctggagg cggatgccag aaaaaccgcc agtactgttg aacaggg
//
LOCUS       DAZKJR010000143        12489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKJR010000143 DAZKJR010000000
VERSION     DAZKJR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.144"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(148..450)
                     /locus_tag="ACHOWS_004180"
     CDS             complement(148..450)
                     /locus_tag="ACHOWS_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049675.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPAKRQVKSPVTTKL
                     VQKKKSAAVRKVSQPSVAGKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            complement(797..1801)
                     /locus_tag="ACHOWS_004181"
     CDS             complement(797..1801)
                     /locus_tag="ACHOWS_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0049676.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKFGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(2059..2490)
                     /gene="tssE"
                     /locus_tag="ACHOWS_004182"
     CDS             complement(2059..2490)
                     /gene="tssE"
                     /locus_tag="ACHOWS_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFQ0049677.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            complement(2493..3029)
                     /gene="tssJ"
                     /locus_tag="ACHOWS_004183"
     CDS             complement(2493..3029)
                     /gene="tssJ"
                     /locus_tag="ACHOWS_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFQ0049678.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(3010..4110)
                     /gene="tssG"
                     /locus_tag="ACHOWS_004184"
     CDS             complement(3010..4110)
                     /gene="tssG"
                     /locus_tag="ACHOWS_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFQ0049679.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(4065..5828)
                     /gene="tssF"
                     /locus_tag="ACHOWS_004185"
     CDS             complement(4065..5828)
                     /gene="tssF"
                     /locus_tag="ACHOWS_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFQ0049680.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLPAGISGFDIE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEADRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPISVCNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(5962..7560)
                     /gene="tssA"
                     /locus_tag="ACHOWS_004186"
     CDS             complement(5962..7560)
                     /gene="tssA"
                     /locus_tag="ACHOWS_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFQ0049681.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKNIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            complement(7560..10949)
                     /locus_tag="ACHOWS_004187"
     CDS             complement(7560..10949)
                     /locus_tag="ACHOWS_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFQ0049682.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTSPRYVTVQDI
                     RHTLRRQHGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPARFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(10942..12090)
                     /locus_tag="ACHOWS_004188"
     CDS             complement(10942..12090)
                     /locus_tag="ACHOWS_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFQ0049683.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREIMDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCFGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(12094..12360)
                     /locus_tag="ACHOWS_004189"
     CDS             complement(12094..12360)
                     /locus_tag="ACHOWS_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFQ0049684.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(12391..>12489)
                     /locus_tag="ACHOWS_004190"
     CDS             complement(12391..>12489)
                     /locus_tag="ACHOWS_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013035836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFQ0049685.1"
                     /translation="MCKGVTKHPDGYVYYGDTPDFIKGKIYPYGNW"
BASE COUNT         3137 a         3526 c         2915 g         2911 t
ORIGIN      
        1 tttattttcc ttctcagtaa ctgaatatct ttgattaacg ccctccggaa atatttatac
       61 ccggaaggct gtccagcttt atgatacccg ccagtacaga attaactgca ggctgagcac
      121 agatttctct gctatttcag tgtcagatta atactgatta ccggaacaaa tgggcagatg
      181 tgtaaatcca ggtttgctgg ccatataaag cggcgcacag gtgggtttac ggggttcagt
      241 gaccggtaca acctcatttt tcccggccac cgacggttgt gacaccttcc ggacagccgc
      301 tgattttttc ttctgaacca gcttcgtggt gaccggtgat ttcacctgac gtttggcggg
      361 tgccacgcca taaccggaac aaaagcgctt cgactgactc agacttccgt cattgcagac
      421 aaaacgtccg tctgacgtgc agtgtgaaat accaccttta gcacccgagc agggttttct
      481 tccccgcgca gcatctgtct gaaccgaaaa taatctatgg tcgcccccgt ttttgcaaca
      541 ctgatttttt aattaatggt gggcttgctt aaatctatcc ggcgtctctg taggcaagga
      601 tgcctgcgcc ccgatgagtt tcgcacctga taatcctaaa aagcccctcg gcttcagtga
      661 gccattttat ttattaggtt tttaatcagg tcgatttgcc gttcatgtca tcaataatca
      721 gtcttcgcaa aaccagacag gcaactttgt atcttattgt ttacaataag ccaattgagt
      781 tatatcagca tttatatcaa gcctgatatt ctttttgctg agtagtgatt acccaggcaa
      841 ttcgagccag tttatttgcc agagcgcagg caaccacatt cgaatgcttc ttgttgagtt
      901 gctcccgaac ccattcggca agttttccat gctggcgttc aagccgcatc atgaatgagc
      961 gggcacactg aaccagtaac cggcgcaaat ttttatcgcc tcgcttactg atacccaaca
     1021 gagtcgattt tcctcccgtg ctatactgcc ggggaacgag ccctgtggaa gccgcaaaat
     1081 ctctgctgca tgaaaactgt ttcccgtcac caggttgcga cgatagtaag ctggcagtaa
     1141 tcggcccaac accaggaatt gtcataatgc gctgagcggt atcatcagcg ttgattgact
     1201 gacttaattc tgattccagc tctgctattt gctcaacaag gtaaaggtag tgggtatgca
     1261 acctcaccaa aaggcggctc agatattcgg ggatttcgtg ttcagccaac acgagagaca
     1321 accgttttat tacagcatca cctgtcggca ggctgattcc aaattccagc aagaaaccgt
     1381 ggatctggtt ggttgttttc accttatctc tgatgagtga ttctcggacc cggtgtaacg
     1441 ccctcatggc ctgcaggggc tcagttcttg gctggacaaa acgcattgat ggtcgtgaag
     1501 ccgcttcgca tatggcttct gcatcaacaa aatcattctt attacttttg acaaacgggc
     1561 gaacgaactg tggagaaatt agcttagttt catgcccaaa atcagagata cgacgagcca
     1621 taaagtgtgc acccgcacag gcttccatta caacaactga gggcggacat gtcgccagaa
     1681 actgcatcag tttagtacgg gtaaattttt tacgcagaag agccttacca aacttgtctt
     1741 gacaatgaat gtggaaggag tgctttccga gatcgatacc aataagtgtg atgttctgca
     1801 tgatggtctc tccgttaaag ggattcacct taacagcgta ctgctgttaa ggtgggggtg
     1861 accatctcat tagtcccacg aacagaaaac agataatttt cttcatttaa aaactcatac
     1921 attgttattg ctgatatgcc agagttatca ggatgatgaa acattccgcc agcgttgtca
     1981 taagccggaa taatgtcaca ttcacacctg acatatatcc atcactgttt atttctgttc
     2041 tgccctccct gctccggctc acgtctgaac gtaccgctgc tgtttcagat ggcgcaacaa
     2101 caccctgccc tccggtataa atgttgttcc gtaacgaacc agcccggcat ctttaagttc
     2161 tgcatcaatg acataatgca actcaccagg ctgagtctgc tcctgcatcg tcacgttgac
     2221 acgcttaata cgtggctcat acttcagcag aacatctgaa agcacacgca tcagttgatg
     2281 cgccgttccc ggcattccct gcagaacggt tgtcatatcc ggcagaccat aatccggcag
     2341 atgtttaagg cttccggccc gggtattcag aatacgctgc atgttatcga gcaccgaaag
     2401 aataacctgc tcttccgcac tgacctggtt taactccagc ccgccagcga aattgccata
     2461 gagaatttca taaagggaag gacgcggcat cactatttat ccttcaccgg taacagcgtc
     2521 agggtattac cggatacttc aataagacgg ggcgtgtccg gctcaagctc atcccggctc
     2581 agcaccaccc gccaggtatt tttcttccgg tcaggctcca gaaacattgc tgccacaccg
     2641 gtaaatttcg ccgcatcatc cagaggcata tccacggtta ctgagccacc tggctgcatc
     2701 catacatctt tctgtgcaat gatatcgcct gccagaattt cactgtcccc cgtaaaaagc
     2761 gcctgataat ccgcgctgtc aaaattccgg ttgtctttaa gctgataaat ccgcaccaca
     2821 accgacaggg gaataccggc agcactggtg ttaatggcct ccctggcacg aatatcaagg
     2881 tgaagtgttt ttatctgtcg gtaaaacagg gatttcgtgg cagataccgt accgtctgcc
     2941 actctttgcg tcaggccaca ccctgataac gacagggaaa acaacgcaat aatcagaccg
     3001 taaccagcct taccagcgat aatcgccatt ttcctgagcc tccttacgtt gataatgttc
     3061 ctgaacccgc tgataacgcc ccagacggat ggtcattacg cgtgccgctg aagtcgttat
     3121 ctttgcctgt gttctcatta cagccgtgcg ccccagttgc ggactaccgg ctgccggtcg
     3181 gcaactgagc cggacatccg gcaacagtga acgctcaaca caaagttgca gacgtacatc
     3241 cagacggctt cccagataaa cgtgcaacaa cgccagcaaa tcagaataca gctgcccgcc
     3301 cggtaaccag ccctgtacct cgctgcccgt ctgtgttgaa agctgtaata acacctgtcc
     3361 gttcacatcc gtagcatgat cgcccatcac cgggcgactc ttcagactga ccggatgatg
     3421 aacacgcatg gccagcggtg ttttcagcgg gatccgtcgt cggtcatgat gccagacacg
     3481 ggcctgtgtt ccgggagcca gcagcgttac cagtgatgtc agcccttcag ctgttcttcc
     3541 gggtaacaac atcaggggca ataacgccag aaaacgcgat accggcgtgg caatattctg
     3601 tgcacatccg gggatcccca gtctggccag accgagcaga tactgggatg ttttatcctg
     3661 cccccccgcc tcaaaggtgg ccgggtacga atatttgcgc cagatacggt aatactgggt
     3721 gatcagacga tgactgaata tatcgagaaa atccgctgcg gcttcatggc cttcccgctg
     3781 ctgtgcaata tcactgatat agtgagccgg caacggagac gtgaccccgt aaagtcccat
     3841 aaacgtcaca tgtacagtgg gtggcagatg ggattcttca ggaattacag catcttttat
     3901 ttcactcgca ggaaacccca ttcccgtgta tggacaaaag cgaaccgctt cctgacgggc
     3961 ctgccagcca ctgccaatgg gaggctgatc tggctggctt tgttccagta actgacagaa
     4021 gcgataaaaa ttaatatacg gaagctgttt ccggtaacgg gtaattaacc gggcaggcgc
     4081 ggattgtgat tttctttcca ccggatgcat tttccttcag gctgaacaat cagtgtgagc
     4141 tgattgaact gattcatatc ggcgtacagg gccaggaaac ggttgagcat ttcgccaaac
     4201 aggtgaatat ctccctctcc ggcaaacccg ttgccatcaa gagttacctc cacatcaagc
     4261 ccccggagca gaaaaccctt ttcaaaacgc tgaatacgat gatgctgtac agccagaatg
     4321 gcatcaagcc gacggtgatt aagttcatcg tcccgccagt tataaagcgc cagcgtgcca
     4381 cgcagcactt ccgcactgct cagcatgtta agaaatcctg tccccagatg actcatcacc
     4441 cgccagtgaa aacgatcttc tgtcggcgga taaaccggca gcgtgggttt acaaagatta
     4501 catacactga tcggggtctg cagtacctgt tcacaccgat caagcagcgt actctgcaac
     4561 gcacgacggg gaagctgacc attggtacct gtaatacgta atgaaagcgt ttcccgctcc
     4621 ggcatccgat ccgcctccca ccgctgaccg ccaaggataa gccatgtgtc atacatcccc
     4681 gtgacacccc gcttcacccg ggtatgataa taacgttcgg gtgcatcatg gcgcagcatt
     4741 cccccacgat gacggaagct ggtaaaaggc acgtactctg cactgccggt tcgcccggag
     4801 cccgccaccg catccactga atatatctcc gtatatccat cctgcagacg cttcgggcgc
     4861 agcagatatt cgctctccag cccattgatg atgagtggat cggcttcaag cgtaaaaagg
     4921 ttaatcaccg gcacacaatg cagacacagg gcatcatctg tcacaggtaa atctgccggc
     4981 caggactgac tcagcaccac ctcaatatca aatccggaaa tacctgccgg caaagaaata
     5041 ttctccagtc cgttcagatg aacaaacata aatttttccc gaaaggtgaa atattccagc
     5101 aataactgat acccactgaa agcactgtca cctttcggcc acagccgatc ttcttccgca
     5161 aaaccaccgg gtgaaaacca tccgtcaatg cggatccgct catcatttcc cggcagcctc
     5221 agatacagcg cagccagacg ctttgtcatc atcagatgaa gggtgctgct gaccggggct
     5281 tctgctgcca gatacaggct cagacgatgc aaatcgacct gagaccagtc agccagttca
     5341 ctgcaggcaa aacctatttt cagcactgag cgcccgtccg gctccgtagt cataacgacc
     5401 ttctccactg ccagtggatt cagtggtatg gcccgggtgg tacggtaacg acagactgta
     5461 tttccggggc caacagggcg ggaagtcact tccagtccgg ctggtacggt ttcagccatt
     5521 ttcatgacgg ataaccgggg agtcagtgcc accaccgaca gggacgggat agtacgcagg
     5581 taatgaggcc agagcatact gaccagactt tcagtaagct ccggcaaatc atcatcgatt
     5641 ttctggcgca gccgtcccat ggaaaaggcg aaaccttcaa acaggcgttc cacgcaggga
     5701 tcaggcgtac ccgctttatc gagatccagc atcgccgccc gatcgggatg agcctgtgca
     5761 aacgcctttc cggcttcacg cagataacgc atttcagcgt caaaataacg cagggttaaa
     5821 tcgtccatta tcttctcaga ataaagctaa ctgttccgga gactcgcgct ctgacttcag
     5881 ccatatttca aacaatcgcc cgaactgaac tgcaggcagg atttcattaa tcagattaca
     5941 catgaatata attcagacat ctcatgaact tcccgaattg cacaacacag cagctctggc
     6001 agcatcaatg gcgatgagcc cggcaagaag atgttccata tcaggctgca gacgcaccct
     6061 gtcactttca gtttttgcgg acttcatacg taacagtttc aggcggcggg cttttacctc
     6121 aaataccagt tcaggttccc attgtgacag ggtcagccgg gttgcacgtt catccagctc
     6181 cacaagcaga tgcagggcaa ggtcattttt tccagtctgt tcggcaacac gggccatcaa
     6241 caggcgcagt agccagttgc tgcggtcaga ctgaataccg ggacgtgcct gtaaccagct
     6301 taaagcggct tctgttcctt cattatccgc aatctgcagg gcttccggct caagagataa
     6361 aatgtcgtta tctccgggag ctgccgttat cgttacagaa ggttcttccg cccagcgttc
     6421 accacactcc atcacctgct gctgtatcca gctcagcgtc acgtcatcgg caaacggcat
     6481 gccgtcgtta aaagccagtg tctcaagccc cggcaggcgc agaagcaatc ctttcaggtc
     6541 atactgaata atggcagcat aattttcttt tccggtctgc aacagcgcct gatgaatata
     6601 ccactgcagg tccagccaca gatgactggc tccccgggca aacatatcgt cacactgttc
     6661 aagcagtgac aaccagttct gctgcagata caaccgtttc agtgacgcac gccggtccgg
     6721 ccccggtggg gcaatacggg tccgtccatc agcagataac ggcggtaact gatgcagcgt
     6781 gtcatgccgg acgcttttca tcagtcgatg agccgccagc cagccctcag gctggtcacg
     6841 cagatatttt gccagaacac gggcctgtga cagcagttcc tgtccggagg tgatggcaga
     6901 taatgccatg acgctgccgg atgttaccgt cggtgccggc gcggcagcct cctgaggcac
     6961 cactgcatca acaccaccgg ccttcatcag gcgattttca agtgcctgat ataacccgtt
     7021 cagcaccggg gcggaagcct catccgtaaa ggtctgttct gccagccaca gcgccccggc
     7081 aatgcgcagg gtatccgcct tcaccacctc cggatacaga gacagactgt ccagtatgcg
     7141 ggcgctgcat aaccactcca gcgccgcctt cctggcccgt gaccgttgtg gatgcaggtg
     7201 ctctccaaaa cgctgcaaca aaccggtcag cagctccaga ccgtcggcca gccctttttc
     7261 accatcctgc cgcagacggg cccagatata ccaggtcgcc acccggatat ttttggttct
     7321 ggtggtcagt aatttttctg caagctggct gacaattccg gcatcggctc cggagagttt
     7381 attgacctct tcacgcatct gctgaaaatc atcatcgtac cccgcatcat cacctgcggg
     7441 attatctggc gtcacgggag caagccattt atcccacagc gcaatacgca cctgagccgg
     7501 ttcagccaca tcccggggtg tcgtctggca tgcactgagg agcgaatgaa tggaagccat
     7561 cagtcagtct cctccacgga atccccggca tcttcgtcat tgcctgtaaa cgctgacgta
     7621 ccggagagtt cgaaaaccgt ttccggcaga acaaaattgc ggagtttcag caatacaagc
     7681 ggcccttccc ccgcttcagt tcgcagtgtg taattcagca tacgaccgtc ctgcgcctgc
     7741 cagctcaggc tccagccact ggcaacgccc ggtgcggctt tccgtcgggc catctcaagc
     7801 agacgtatca gcccccagct tcccggcagg tctgcatact gacgggtacc ggcctgagtg
     7861 cttacccagc ttaaactggc gccaggtgct tccgtatcag ccggccaggt aaatcgcttc
     7921 cataccggca tctgattaac ataaatcagt ttctgattat cggttatcag cgtcgtctgc
     7981 atcacaccgg cagcagttcc cgggcgcagc tcaaaatgca gccccgcgtt acctgtggtg
     8041 aacgccacat ccgctatttc actcagggta ttgatggctt tcagaaacgc agggttgaat
     8101 gtcagtcccc gggtattaat ggtatcaggt acccagcgac tgccttcact gtgcaacaca
     8161 ccactcaggt tattctgcag gaaacgggca atacgtcccg tgtccgtatt caggtatttt
     8221 gccagcaggg gaagcgaagc atcgctgctg acatttttaa acggataacg cccggaaaat
     8281 gcattattcc agccatccac caccgcagta cgccaccggg cattcagact ttctgccgcg
     8341 ggcgtaagaa cctgctgcca cgcctgctcc atcgggcgaa caaagaccgt ctgtccgaag
     8401 ccataccact cctgtccgag tccggcagcg gtcaggctac cgtaatcccg ggtatcggtt
     8461 aaatcaaccg atttcccctg caatactgtc tgtgccagca tctgcatcat ggcctgcggg
     8521 tcagaagagc cggcaatctg ttgcagacgc agacgaacct gtgtcacccg ggtcagatat
     8581 gtctgcagat tcagcatatc cgcactgttg ttctgattat ccatcagtgc aagcaccggc
     8641 ccgaaagtcg tcgccagagg accgtgaagc cggctttccg gtactgccac tggctgtttt
     8701 tcctgtgaaa gcaggtttct ggcagattta accagtgaat cagtaaccgc ttcccggggc
     8761 tggccagtac gcccctgaac cgccagcgta ttcatcagag caaccagggg ggactggcgc
     8821 acatcagcca tcagcgtcag ttgctctgtc acatcagaca gcgtctgagc tttacgcaag
     8881 tgcagactgt tcagaaaatt cagccaggca ttaccaaaat cagcaaaata gcgcgttgtc
     8941 aggcgctgtc tgagtgcttc cggcgatacc ggtgacggtg ccttctgcct gccatccgtc
     9001 agtacccagt ccatttcttc acgacgctca ttaatcacgg tgtctatcga cggcaatacg
     9061 gcctcttccc aggcctggcg ggtaaacata ccgggaacca cttcatccgt aaaaaagagg
     9121 cgggagacat cagtatcacc ggtcatatcc gtcagacgca tatccgcaaa ctgatgtgcc
     9181 acccgtgcca gcattttctg atacagcatg gcatcgctgt tctgtgttcc cagatggcga
     9241 agcagcagag ttctgctctg gctgaccagc tcttcatcag ccgtcagctt ccactgagga
     9301 tgagtaataa tcccggaggc ataatacgcc agcaacgtcg ggccattagc ctgccagaaa
     9361 acggcactca caccgtcacg ctgtggccat tcccgcatga gcgtacgact gaacctggcg
     9421 ggctccatat gttgcggttg cgccagcatc agatacagtc tgagctggtc atatgcggct
     9481 tttgccattt tcccccgctg cgggctgtcc ggaggaagac gaataaatgt cctgagctgc
     9541 tgctcaagat gagcagcagc ggcatcacgc agtaaaagat gtgcattttg tgcataaagg
     9601 ggcattacga ccgcgagcag gtctgcattc tgattcatac cggcccgcag gtaccatggc
     9661 gccccgtgct cctcccggta ctgaaggcgg gccagggatt tctgcagatt cagcaacgcc
     9721 tgcagacgtt ctgcaggaga cagttgctgg ttctgagccg gacgaatctg ttcctgtact
     9781 tcagcaacca gactccggtt tgccagaaag gaaaccacca ttcctgttcc ctgagccatc
     9841 atcagtatgg ccgccataac ggccagactt ctgcgccagt tatgacctgt gcgggaaggc
     9901 tgtagccgga caggtaattg ctgcactgtc tccggcaacg cctcccagcg gttatccatt
     9961 cgccagcgat gtctgacact tcgttcaccg ccgacagttg ccggactgaa aacaataccc
    10021 gccagcaaca actgacggta tggacggagt aatacggata atggctccac cacagcatca
    10081 atatttcgtc tgaatcgctc tgccagcgac aacaaaaaat aataccgagg ggcacagcat
    10141 atctgctgta tcccctgagc aacaagcccg gggagcatgg cctgtagttg tgctttcagt
    10201 ttgtcagaac tacattctgc tggcagaagg catcccacgg gctgagtaat acgccctcgc
    10261 tcatccggac tctcctgcag tgaccagaca tacagcggca gacgccagcc aaggcgctca
    10321 caacatgaac tgaccgcacg ggaaaccctg tcaagggcat cttctgtcag tggtgcggat
    10381 aatgtttcac tcagtccgga agttacccag acaatgccat cagcaggccg gtaacgcaga
    10441 cggcgtaacg cagccagcca gtctgcgttt tccggctgcg aggggtctcc tccccagaga
    10501 agcaatgtcc cctgctcttc ctgccagaac tgttctgtca ggcccggggt cagcaactca
    10561 acctcagagg cagtacctgt tatcaacaga atgcgtattt tacgtcccca gaatctgcca
    10621 tgctggcgcc gcagagtatg acgaatatcc tgaacagtaa catagcgcgg tgacgtctga
    10681 gccagtcgct cttcatcgtc aggtaacctt tgctccttac gggcccgacg tcgatatgtc
    10741 agttcctgaa catatttttt catcacaggc aggtaacaga caaagagcaa ccccagactc
    10801 agcaatatca gcagtagtat ttgatcccgg cgtgtttctg cccccagaaa tgctccatat
    10861 cgggttatca gccaccagag cgcagccaca ccaataaata caatccccgg tcgtccaaag
    10921 cgaccagaca gacaggccag tttattcatt ctggtttcct gatgtaacag tactgataaa
    10981 atgttcttct ccggatgaga ataaagtcag gagagaatca ttaccaaaac agaccagatc
    11041 ggctgccaga gcagtaagca gccacggagc agcaggtccc gggataccgc accatttttc
    11101 cagtaacata cgtccggacg gttcccatcc ggcaccatac tggtttccaa tagtcatcag
    11161 cggtgcagca atcttttccc agtgataaca gtcccccagt acacgagcgg tacgacaggc
    11221 agctgtctgg gtttcaagga aaagtgtgaa ttcatcatta aacttgttga tatccagcga
    11281 catcgggcgt agcagacggg ctggatgcgg caggttatat ttttgcgcaa catcatcgct
    11341 ggtcaacaaa agtgcagcca gtccgtcaga gtatgcattt cccccattaa gctgtattac
    11401 cagtatcaaa tccacagtca gcactggttg tttcagacgc tcatcaaccc accccatgga
    11461 aaagtcggga gtaacctcga tattgtcagg aacaacacgt tggggaaata acacttccca
    11521 tgcagaaaca aaagcagatc tgatttcaga atcaggctga tcggttatca gcgtcacatt
    11581 tacagggaga acggcaggta actgacaaaa tttatccatt atctccctaa gcaagacacg
    11641 taaagaggct tcaaccttat gcccactgtc agaaagataa tctattttct ttacaaggcc
    11701 gtactgcaga ggcaattcat cacaaagagt agctaccgtt attttatcag gcaaataaac
    11761 acaactggca gtaataacaa gatacctttc ggcccaggat tcccactgtt tctgagcata
    11821 ctccccctct tttttaaaga acaaatgttc atccatctgg ttgctccact gccagtgtcg
    11881 caggcaaaac aggaaaaacc agccccccgg aggagtcaat gcaagccacc agatactgaa
    11941 atcatttaat gctttcacat attctgatgc ccggataata aacaacagag caccaacaac
    12001 agccataaat gcccctgtca taaaccacaa catcattgat ggtttatgag gtaattctgt
    12061 tgataatgat tttgttctgc tccatcccat gggttatcct attgcacatt caggtgcact
    12121 ggatataacc ttacacccac acagacattt acatccatca atcacaacag ctcttccatc
    12181 agatatccat tcaggagagc cctccacaat aggattagta ccatgtccgg aaacaggaca
    12241 actaaccata tctccgacaa gagctacttt tttaccatcg acaatcatgg ttgaagatgc
    12301 cgaaataact ttaccaccat gtgtggtatt atcaccaagc aatacaaaac ctttagacat
    12361 aaaactctca aaaataaatt ttactttgag tcaccagtta ccgtaggggt aaattttccc
    12421 tttgataaaa tctggtgtat caccgtaata aacatatcca tctggatgtt tagtaacccc
    12481 cttacacat
//
LOCUS       DAZKJR010000144        12424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKJR010000144 DAZKJR010000000
VERSION     DAZKJR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.145"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(128..865)
                     /locus_tag="ACHOWS_004191"
     CDS             complement(128..865)
                     /locus_tag="ACHOWS_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFQ0049686.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWMHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESTFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(819..1019)
                     /locus_tag="ACHOWS_004192"
     CDS             complement(819..1019)
                     /locus_tag="ACHOWS_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0049687.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            1134..1596
                     /locus_tag="ACHOWS_004193"
                     /pseudo
     CDS             1134..1596
                     /locus_tag="ACHOWS_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1635..1880)
                     /locus_tag="ACHOWS_004194"
     CDS             complement(1635..1880)
                     /locus_tag="ACHOWS_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFQ0049688.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENEPQPVEQPEPVQPGTDGTMM"
     gene            complement(1916..2095)
                     /locus_tag="ACHOWS_004195"
     CDS             complement(1916..2095)
                     /locus_tag="ACHOWS_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFQ0049689.1"
                     /translation="MTYINVCLLYFCTVVCCLLLVSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            complement(2153..4210)
                     /locus_tag="ACHOWS_004196"
     CDS             complement(2153..4210)
                     /locus_tag="ACHOWS_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFQ0049690.1"
                     /translation="MNIMTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMT
                     FADCLISFNTQDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTTEPSDWDNAETHT
                     RPAGNETASSSPSWYYVIVIAGQSNASSYGEGLPLPDSYDRPDPRIMQLARRNTQTPG
                     GIPCKYNEIIPADHCLHDVQNMSLLNHPKADLKKGQYGCVGQGLHIAKKVLPVIPADA
                     GILLVPCARGGSAFTTGAVGSFDPASGAAEASLRWGVDTPLYQDLVSRTKAALEANPK
                     NVLLSVVWIQGEGDLKATPAQHGAQFTAMVKKFRADLGAFSGQCIGGSSDSVPWLCGD
                     TTYDWKEKNPAAYETIYSGYKNRASENIHFVPLMHDEFGNKTATNLPEHDPDIASAGY
                     YGAESRGDGNWTTASRATHFSSWARRFLVSDRFASAILKYAGLTSAFIAGVAPDGEAL
                     PQPAPGGKPEAPTGSQPGGSESPHPTSPSLRTLLSLKASAGEAPAQGWKITDGTIQLT
                     DEGKALTVSKQSGKTWTLTHGTDAAGELLKHGGQLTCKFRLSGEVKEGQFALGIYLHT
                     DTVPEGVTMSGTGTPFLMAYYLQTTGGQLNLMHHKTGGNTKLGSFGAYNNQWHTLVLK
                     FTAGSAQVTPVLDGTPGQAFQMVKDALSPAKDTLTLTDVTKGATYGVSFESVVLEVNT
                     PAA"
     gene            complement(4301..4861)
                     /locus_tag="ACHOWS_004197"
     CDS             complement(4301..4861)
                     /locus_tag="ACHOWS_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0049691.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            complement(4875..5061)
                     /locus_tag="ACHOWS_004198"
                     /pseudo
     CDS             complement(4875..5061)
                     /locus_tag="ACHOWS_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5084..5287
                     /locus_tag="ACHOWS_004199"
     CDS             5084..5287
                     /locus_tag="ACHOWS_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0049692.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
     gene            5367..5888
                     /locus_tag="ACHOWS_004200"
     CDS             5367..5888
                     /locus_tag="ACHOWS_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFQ0049693.1"
                     /translation="MELINVKRYYPEHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(5923..6834)
                     /locus_tag="ACHOWS_004201"
     CDS             complement(5923..6834)
                     /locus_tag="ACHOWS_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0049694.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTQD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEISWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(6834..7394)
                     /locus_tag="ACHOWS_004202"
     CDS             complement(6834..7394)
                     /locus_tag="ACHOWS_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFQ0049695.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            complement(7385..8467)
                     /locus_tag="ACHOWS_004203"
     CDS             complement(7385..8467)
                     /locus_tag="ACHOWS_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFQ0049696.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRIIGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            complement(8467..8904)
                     /locus_tag="ACHOWS_004204"
     CDS             complement(8467..8904)
                     /locus_tag="ACHOWS_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFQ0049697.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            complement(8897..9511)
                     /locus_tag="ACHOWS_004205"
     CDS             complement(8897..9511)
                     /locus_tag="ACHOWS_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFQ0049698.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            complement(9501..10625)
                     /locus_tag="ACHOWS_004206"
     CDS             complement(9501..10625)
                     /locus_tag="ACHOWS_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFQ0049699.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            complement(10609..11958)
                     /locus_tag="ACHOWS_004207"
     CDS             complement(10609..11958)
                     /locus_tag="ACHOWS_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFQ0049700.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGKVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            complement(11945..>12424)
                     /locus_tag="ACHOWS_004208"
     CDS             complement(11945..>12424)
                     /locus_tag="ACHOWS_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFQ0049701.1"
                     /translation="GLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAAPVL
                     LDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTEAWQ
                     KLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYGLW"
BASE COUNT         2960 a         3434 c         3009 g         3021 t
ORIGIN      
        1 atagcgcggc gatttttttg ataccgattt gcatttcatt tgataccgct gaaaaacgcc
       61 gatacgcaac ttatttgata ctaaatcggc gcttttcttt tgagttttag aatgagtttt
      121 taagcgctca ttcactctct ttcaaaccag ttttaactgt ttgtggtttt gggtaaggct
      181 ggactttgaa tagctttgtg ttgaggcgtt ttctggccct cttgttcaga aaccggatgt
      241 aacgaaactg tcggaactta tgcacactgg ctctgtcgat attagccctc agatgttcac
      301 ctcgttgtcc gcctcgttta atggcattcc tgcaaatctc gtgataccac tcgccatcaa
      361 gttcatagaa tgttgattca tggctgccta cataatcaaa attgctggcc tgatacacga
      421 caccaagaca gccacaacgc tcatctgcaa acgactgaac ccactgtacc tgcggataaa
      481 gctgcctgat tagcttgagc gcgtaactga ttgcccttga ttcggagttt ctcggcatac
      541 agtcatgcat ccatagccgg ttaagctcca tatattctcg gttctgcgta cccgttacga
      601 cgcgtgcacc actgtttggg ttaagggcgt aaccccattg cattacgcca acaagttccc
      661 gttctgaaaa tatgcccaga tgaaggtagg aattatttac gaaacggtgg ctgtaatgtt
      721 tattgacgat aaccagtctg gccagccagc aacttattgt ctcaacccgc aactcacggg
      781 agccataacc aacgatattg tcgttatagc gaataagttc aggcgtgctg atgatacggg
      841 atgtgacttg ctttctgttc cccacgacag gatttccttg tgtatgtggg gtgctctgtg
      901 gcgctcggag atgtgatttg attgagggtt ttacagcgcg gacattttac ttccagataa
      961 ctgaaactgg cccgtgccag tagcttattg cagtgtcggc accgtatgtt tcgatacatg
     1021 gccgcataac ctcctgcttt gttgttgatg cctttctcat gcaatcgatc gacaaaaacg
     1081 atcgattcga tttgtttaat tgatacaaca aatagattta aaagtcatca acaatgattc
     1141 agggcggtga ttatttactt aaagaaacga agagttaggt ggtgaaaacg atcataaaat
     1201 gatcatttta ttgacttttt atacagtgaa ggtaggatcg cgcgtatatg tagtatgttt
     1261 caaatcaaca ggatactaca taaaagggaa acccagcata gctggaacta tgctgggtca
     1321 atctgtaaaa acttgcatca atgctttact tcgagctcgt ggcgtatcct atgatattcc
     1381 ccggtctcct gtaaagcaga gatgcataaa aaggagatct ttatcatgga taaagagcct
     1441 gtttgccctg cctgtaagac tcccttacag ctcatctatc gccgctctcg atctcaaaat
     1501 ggcaagacat acaaaccaaa aaatgcttcc tgtttcagga tgctgatatg tcctgcctgt
     1561 tctccccgga gggaaaatac acaaaacgtg cattaacaaa ttcacccggc agggaaacct
     1621 gccggtcggt attgttacat catagtaccg tcagttccgg gctgaaccgg ctctggttgc
     1681 tctacgggtt gaggctcatt ttccgtgctg ttttcagccg gagaacgacc aaccattcgg
     1741 gccagcactt catcaacata tgcgtcgatt tgtgcctcaa aatccttgcg aacctgcgcc
     1801 ttcagcacgc ggttcacttc ttcagaataa agtgcctgtt tgacaagctc ttcagtaacc
     1861 gtgcctttaa tctctggcat gaatattctc ctttcctttt ctggttcaca aaaaattaaa
     1921 tctgaccgga gtctttcggc tgcggcatta cagcaccgct ttttatcttt tcctcggcag
     1981 ctgcatcaat tttatttcga aaataattgc gaataatttt ataaccaccg ctgacaagca
     2041 acagacaaca aaccaccgtg cagaaataca gcaggcagac gtttatatat gtcatataag
     2101 cctcactctt attaactgat gttacagccg gataaattcc cggccgtcac gattatgccg
     2161 caggggtatt aacctcaagg acaacgctct caaaagacac gccataagtt gcccctttgg
     2221 tgacatctgt cagtgtcagc gtatctttgg caggggaaag tgcatctttc accatctgga
     2281 atgcctggcc cggagtgcca tccagcacag gcgttacctg ggcacttccc gccgtaaact
     2341 tcaggacaag tgtatgccac tggttattgt aggctccaaa tgatcccagt ttcgtattcc
     2401 cccctgtttt atggtgcatc agatttaact ggccgcctgt tgtctggaga taatacgcca
     2461 tcaggaacgg tgtgcctgta ccactcatgg ttacaccttc cgggaccgta tcggtgtgga
     2521 ggtaaatacc cagagcaaac tggccttctt ttacttcacc tgacagacgg aatttacagg
     2581 tcaactgacc accatgcttc agaagttcac cggctgcatc agttccatgg gtaagcgtcc
     2641 aggtttttcc gctttgtttg gatacggtca acgctttacc ttcgtccgtc agttggattg
     2701 taccatctgt aattttccag ccctgagccg gggcttctcc tgctgatgct ttaagtgaaa
     2761 gcagcgtacg gagtgacggg gatgtcggat ggggggactc actgcctcct ggctgactac
     2821 ctgtcggcgc ttccggtttc ccacccggcg cgggctgagg cagggcttca ccatcagggg
     2881 ccacaccggc aataaatgca gaggtcagac ctgcatattt aagaattgct gatgcaaaac
     2941 gatctgagac cagaaaacgg cgcgcccagg agctaaaatg ggttgccctg gatgctgttg
     3001 tccagttgcc atctccccgt gattccgcac cataataccc ggcagaagcg atatccgggt
     3061 cgtgctccgg caggttggtc gcggttttat taccgaactc atcgtgcatc agtggaacaa
     3121 agtgaatgtt ctcggaagcc cggtttttat atccgctata aatggtttca tatgccgccg
     3181 ggttcttctc tttccagtca taggttgtgt caccacataa ccacggcaca ctgtctgatg
     3241 aaccaccaat acactgaccg gagaaagcac caagatccgc acggaatttt ttcaccatcg
     3301 cagtaaactg ggcaccatgc tgggccgggg tcgctttaag gtctccttcc ccctgaatcc
     3361 atacgacaga taacagcaca ttctttggat tcgcctcaag tgccgcttta gttcgactga
     3421 caagatcctg atacagtggg gtatccactc cccagcgtag tgacgcctcg gctgcaccac
     3481 ttgccggatc gaatgagcca actgcgccgg ttgtaaaagc agagccgcca cgggcacagg
     3541 gaaccagtaa aatcccggca tcagcaggaa tcaccggtaa tacttttttc gcaatatgaa
     3601 gtccctgccc cacacaacca tactgccctt ttttcaggtc tgcttttgga tggttcagca
     3661 gactcatatt ctgaacatca tgaaggcagt gatctgctgg aatgatttca ttgtatttgc
     3721 agggaatacc tccgggggtt tgtgtattcc tgcgtgccag ctgcataata cgtggatcag
     3781 gacgatcata agaatccgga aggggcaacc cttcaccata agatgaagca ttggactggc
     3841 cggctataac aatcacgtaa taccaggaag gggagctgga agccgtttca tttcctgccg
     3901 gacgggtatg tgtttctgca ttatcccagt ctgacggttc agtagtaccg gcaacatcag
     3961 cacttaacgg aatgacagca ccaatacccg ctccggtaat ttccacgttc ggatcaccgg
     4021 atactggcct tacccacagg caatcctgtg tattaaaact gatgagacag tcagcaaatg
     4081 tcattcctcc tttcgtttca gcaggaggaa agggagcctc aagaaatgcc gctgtaccac
     4141 cggaaacttt aacggtaaag tttccgggca ttgatgatag ttgaacccat ttggacgtca
     4201 taatattcat cctgttcctg aataaacaga attatcatat ccacagaccg ataaacgcca
     4261 ttttgcgtta attcagtaaa ggctcaacaa agggagcgtc ttatttttcc gaaacgctcg
     4321 caggataata acgataaagg gttgaaaggc cgatatcaaa aataagtgcc acctgtcttc
     4381 gactgacacc cccggccagt aaacgcccga cctgctcgta ttgttcctgc gtgagacgac
     4441 ggggtctgcc gccggttcgt cccctttcac gggcagctgc aagaccagcc cgtgtccgtt
     4501 cgacaattaa ttcacgctcc atttctgcca gcgcacccat tatgtgaaaa aagaagcgcc
     4561 ccatgggggt tgacgtatca atgttgtcag taagactacg aaaatttatc ccctgttgct
     4621 gtaattcatc tgtcagcgca acaaggtggc gcatactgcg ccccagcctg tccagtttcc
     4681 agaccatcag tgtgtcaccg ggttttagtt tcttcagtgc ccgcttcagt cccggccgct
     4741 gtgtcgtttt accactcatt ttgtcttcaa aaatctgctc acatcctgca cattccagcg
     4801 catttctttg taaaccggtg ttctggtcat ttgttgatac gcgtatatag ccaatcagca
     4861 tgtatttacc ttccttaaaa agaggtgaaa atgcctttta aaacagatat acggtatccc
     4921 tgaaaacctc ggtttacggg aaacggtaaa taaggcggcg aatgccctgc catcgaacgg
     4981 aacggctgtt gcagcgaata aactcgccac tccaagaaat attaatggtg tcccatttga
     5041 cggaacgcag gatataaata ttacatcggg gatgacgcag tctgtggctg actcacgtta
     5101 tgttcaaagc attcgaagag gttcaaggtc aacgattgga atgcaatata atatttttga
     5161 agttcctgac ggatgcgtac ttacagggct tgatgtcgct ggtgatggta atgcaacggt
     5221 aaccgcatat tatcgacctg ttcagtttct gatagatggt tcgtggaaaa cagcttccag
     5281 cgcataaaag tgcagtagtt caaaaaacag attatctgaa taaatatatg atgattttgt
     5341 cttttcaaca aaaacaaggt gaaataatgg agcttattaa cgtaaaacga tattacccgg
     5401 aacacaaacc ctatggtgag gatgttcagt atttccagag tgaggacgga agggattttt
     5461 atgaatctat cccgcttttc acaaagaaat acaaactctg tatttcgcct gtaacgggta
     5521 ttatctgctc tgttgctgaa gatgtctctg ctttatatcc ggcaggtttt actgttgttg
     5581 aagtggacga actgccggaa ggtgtgaata ttgacggtaa ctggcagttt tctgatggcc
     5641 tgatatcaaa ggtgccggta aactggaaga cggttgctga aaagcgtcgc tcatcgctgc
     5701 tccaggaagc caacgaaacc gtggatgact ggaaaacaga attaaaactg gacatgatta
     5761 gcgatgaaaa taaattacag ctgacgcgct ggatggcgta tatccgacag cttaaagaaa
     5821 tgcactttaa tgatattgcc agtgaggggc attaccaggc gattccctgg ccggaaaaac
     5881 cggaataatt cagagggtgc attcagcacc cttttctgtg gtttacactg aacttaccgt
     5941 tctccaggca ttattaatat aaatctgaac cgggcgataa tagaccccac cgatattgtc
     6001 cgcagaccgg gagccggttt cctgcacgtt aataccggaa agcatacaac cggaaggtgc
     6061 gctgtaattc catgatattt cattgcctcc gggtgaatga tagctctgtg ctccaagctg
     6121 aacattctgt acataccggc catctgcggt tgactgcgtc attcctgacg tgatgttgat
     6181 atcctgcgtt ccgtcaaaag gaacgccatt aatatttctt ggggtggcga gtctgtttgc
     6241 tgccacggcg gttccacccg atggcagggc atcagcagcc ttatttaccg tttcccgtaa
     6301 accgacgtta tcgataaaag cgcctttatc cggaatgtct gcgccgttct gctccctgcg
     6361 caggtaacgg ttatcacctt cgcttttgct gtaaacatcc agattattac gggcggttcc
     6421 cgcattctcc aggtcggcaa ggttctgatc cttcgccaga gcattaacgt caccgggacc
     6481 aagactgttt tttgtggcaa gcgccccaag accaagataa ccacgggcaa gacgctgggc
     6541 ttccgcaccg gcatcagcaa tttctttcag gttgtttttc cgggaaaagg cattcgcagc
     6601 gataatggct ttaatggaca gcgtaagctg gtttaactgc gccttatccg gctgaatact
     6661 ggcttcagcc agaatattca gcagttcggc ctggagaata ttcagccagt cctgaccaat
     6721 ccagctgata cctttttgtc cgtcaccttc ggtaaaccag gtggtggtat tactctgcgc
     6781 cggtgccagc gcaggcatat tcgccacgcc ggaattatta tcaacatgaa acattaaata
     6841 gtctcctctt catggctgtt tgcatacacg taaataaacg tctgccatgc aggcttataa
     6901 cggttcagaa tacattccag cgcaccgcct tcataaatac gcagcggcgt gagaatatca
     6961 tccagcacat tcatattccg gtagccggtt tcactgttaa tcgtgacaat actgacccac
     7021 tgtgaatccg gtgatggctg aatatctatt tcataaccaa actctgccgc aaaccggata
     7081 taaaattcac ggttaagaga gggtttcatc cggtatttat tcccggcata acgctgacgc
     7141 tcctgaatgg ttgcgccggt catatcacat tccggtaacc ccagataacg ctcccagtct
     7201 tccagcagca gacgggatga atcaggaaaa cgttctgcca gcatctggtt accggtccag
     7261 gaaacacgct ccgtggaatg actgaggccc aggcacaaag ccgcaagaac ggatgacggc
     7321 cgcttatccc atgccagccc gtcaggcagc aactgcaaca gggcacgctg atgcggggtc
     7381 agggttacag ccatgtgatt tcccccacgg tcagcaactc tgtatctccg gccatcacgg
     7441 acttcagcgg gctgcgaact tcaaaatcct ccagtccttt cacccctgca acagcccgcc
     7501 agaatgatga aggcaataca agtccacccg gccgcgattc gttataaagc aggtccgtta
     7561 atgcctgttt tacggcagcc tgattttccg gcgttttggg tacaatcctg atctcaaacg
     7621 ccaccggctt attggtcagc ttaaacacgc tgattgtcgg cccaagtggc tgaccaacgg
     7681 gctggcccgt cgccggatca tcatgactgc ggatataatc cgccacccgc ttcacatcac
     7741 cttctccggg gaaaatgtca gggttattat cctgaacaaa agtcacacca acactacccg
     7801 cctcaggcca ttcaggctta caccatgccc gcgtcacgcc cggtacttcc cgtgcccagc
     7861 gttcaaaatc atactgtgtt ccccctgatg gcgggttctg tacccggaat accagcctgg
     7921 acagcaactc cggcaccgtt tccacatccg caccaccggt gagtcccgtg ccggtgaccg
     7981 tggctgtctg gttgatgccc gcctgtggcg tgataaagga caggcgggtt ccggtcgggg
     8041 tatttccagc gcgccccgca ctttccgcct ccacattaac attcagtgtt ccggcttttc
     8101 cggtcgcaga gccgatgatg cggtacacaa caccatcgct tcgctgaagg agtacgcctt
     8161 ccgtgatccc tgcatccgtg gtcagcatca gttgtaccgg tccgtcagcg cgggaagccg
     8221 gtttacggat gacaccccag aatgcgcagt gtttcaggag ttcggcttca tcagcttctg
     8281 tcggaattat ctgccgggag atccaggcca gatgttcatg ctcctgtgct gacagacccg
     8341 ccagtgcata cgcaatggca ttcagggtgg tttcattcac gcccggctgc gaaccgggca
     8401 ggcgctggct gatatcctgt tgtgtctgtg tgatcagttg cgccagtggt aagggctgat
     8461 aaggcatcat tcccccttca tatcagcata aaaaatcatc ggatgtctgc ttccgtccgg
     8521 cagggtgatt ttcacggaaa gcgaaaggcg agcatgcccc acacgcatgg catgacatac
     8581 cacggatgtg gcagcaccgc ttttatgcag ccacgccagc gcttcatcag cataggcctg
     8641 tgcacggctg accacggagg acagcgtctt ttcacggctt aacagccaga gacgggagcc
     8701 aacggggcgc tcccggaaac tgtctcccca ccatccccga cggtcacggg tgccgtcagg
     8761 gatttcatcg gaatccagcg cccgcctgtc cgtaaacagt gaaataatga ccgcagtcag
     8821 caggctgtta tccgtcagaa gatctgcgcc gttcagttgc agggaaccgc aaccctgatc
     8881 ccagacgaca gcaatatcag ccattctctg gtcctcccgt tttgctgcct tcaccgttat
     8941 cgtgatgcac atgacctgaa taggtgatcc cggcaataac cgcttcggac atcgtgaacg
     9001 tcccctgtga tcttccggtg ccgtcaatgg acacattgcc tttgacatgc aggttcttat
     9061 ccactgtgac attaccggta aagtgagcct ccggtgtatc cacctgaaca ccttcatcgg
     9121 cgtaaatctc cagcgtctta caggtcacaa tgatccggcc gttctttgtc agacgtatac
     9181 ggtgcccctc atggtgatac acgccggtat ctccggcggt aagtccggtc gggcgactgc
     9241 ggcggtcttc caccacaagc accacggcct gatcccgttg tccgccagca caggccagaa
     9301 aaacttccgc accaggcagg gggacgctga tttgcccgta ctgctggggg cgttcaacgt
     9361 catcaaaggt ttcaccatcc ataccggtga tttgcacatt ctggattttc agcgtgtccg
     9421 ttgtgccggt cagtacacca cgcccgaaca gcagacgaac accccgcatt acgggggcca
     9481 gaaggcggct gatgacttcg tcattcatca ttgaatttaa tcccctgttt tttcatctgt
     9541 gcccgcacga aggcgtccac gtcacccgca tttccgcttc cgctgtttcc cggctctgcc
     9601 ggaacaataa agccgtcacg gggtgccaga atcagacggg tggtttcgcc gtgatttgca
     9661 tccagcgaaa actccacttg acagacaaga agatcacgct cttccactcc ggtgcgggga
     9721 gccgtgacag ccgtcagcag gttgatatcc cacaactggc cgttctcccg aaaccagtgc
     9781 cgcacggtgg ctgtcaggcg cacagagcgg gcaaccgccc ggcgcatttc ccggacagcc
     9841 ctctggcgtg caccgtctgc atcaacagca tgatccgcca gcacaatttt cggtctgtaa
     9901 cggtggatgg cactgtctgt caccgtacca acgggtgcgg ccagcgttcc ggctgtcagt
     9961 gcatccccgg catgaccacc accacgcccg tgcccccgta cacggtattc actgtggcgc
    10021 agacggtgat ccacgttgtg atccaaatcc agcagatttt cgcccagcgt cagcgtgtcg
    10081 ccacgctgac tgccggcctg agtgaaaacc agttcaccgg cggcattact ggttaccagg
    10141 acgcctctgt gtctcgcggc ccgtgtcagc gcatctgcca cggtttctga gttttccagt
    10201 gtgaaggtgg aaaagggccg ggctgccgtt gcatcattta cctgccagcg caccgtgacc
    10261 ccgaacggag cacacaaatc tgaagcaatg tgctccagcg tgcggttcct ccactggctg
    10321 cccggatgaa tggctgaaca gtccaccagg tcaccggttt tatcccgtcc gctgagcgtg
    10381 atctgaaaac gcgtggcgct gatgcgctgc cggacctgat ccagccagcc agtgatcaca
    10441 ggctgaccgt caatgcgcaa ttccagagac tgaccggcac ggacggatga cggaaggcgt
    10501 acaccgggca tcattacccc cagttcaaag gagcccgcca gatgttcaag agagcgacga
    10561 acactcaccg tcagccagcc ggaaaaaatc tcaccgccca gatacagttc aaccctgctg
    10621 ctcactaatc acctccactg aatggcctcc gggaatgaac agcggatcaa caataccgtt
    10681 acggcgcaca aaacgttgcc agccggtact gttcccggtg gcacggtaca gggtcaccag
    10741 tgcaggctcg gtggtacgta cagtcactac tgttgcaccg ggtagctgaa ctccccgtgt
    10801 attcagatcc tctgtcagtg ccagtctggc atcacgaagc gtcagcgccg tggcggtata
    10861 cccctgcccg gacagtgtca tcacctgccg ttcgagcgct tcgcttaagt cacggttaat
    10921 gcgctgaaga tcaactgaac tttccagcca gacaggccag gtacggtggg ctgcgtcact
    10981 gtccggcagg gtttcctgac tgagaacctg atccagcagt tttcccacgg tctgtgcctg
    11041 ggccaccacg gcagcactct gcattaccgc ccgtatcaga cgaatattct tttgtgctgc
    11101 cggtgtcaga ccgatgagag tctgcggatt atcctgctca tccagcacgt aacgcaggga
    11161 agataatgtc ctgtacagtt gcggcattcc ctgagagacg gcggatgtcg tccggccgat
    11221 actgccaccg ggccgggtat tcaggcctcc tgaagacagt gacggcagcg atggcagggt
    11281 gatcagggca ctgaaaagcc ccgcaaaaga tgaggccatg cgggaaggtg ctgtcagtaa
    11341 tgccgtggca ttccctttca gcgccgtgaa cgtggcagta aaggcactga tatcctgtac
    11401 aatccccatg ccggaaacgg cattttccag ggcggacacc ttatcgctga tggtttcggt
    11461 cattgcctgg acatcgtgca ttccgtcaga aatcaccgtc cagcccgctt ccagggtttc
    11521 aaaaaccttc ccgagcgcgg tggtactctt ctgctccaga atggcggccg tgtcctgtgt
    11581 gacagcaggt gcggtatcat cacttaccgg cgtgacatta atggtgaact caaccacgcc
    11641 ctgttcagca gcgttataac gactttcaaa gctgtttatc agcacattta acgtgccgta
    11701 atccgggtga agcagctccc cggcaccggg agcacgaagg gcatcacgca gacgatttcg
    11761 ctgtgtctga acatcatccc ccatcacaag tacactgaac gtaaattcag ccagtttagg
    11821 tcccagatcg tctgcaccac cggtttcccg taacggatat tcccgacgga caatgtttcg
    11881 cccgccacgt tcgcgctgct cacgccagac gaggaacggc acaccacgga acgcaccacg
    11941 accgtcacca gaggccataa ttccctccgt tccagacatt cacatccagt ccgtgaccgg
    12001 acgtgtcatc aatatcaata ctgcgagcct gccacccttc cggggcaacc agctccacac
    12061 gggcagaggc tttttgtacc gcctgtccac tgctttcatc atcactgccc cgtaatttct
    12121 gccaggcttc agtcagccat ccccccagat agttaccgag atagctgccc accgtagaac
    12181 caatggcaat ccccacaggt ccggctgccg cccccagcgc accaccggca atactgcctg
    12241 caagagagcc gaccgcttct cccttgtctg tggcactggc gctgccatcc agcagaaccg
    12301 gggctgccat cgcgccagcc cacagggcac ctccacccag ccggccggcc agtccaccgg
    12361 cactgcggaa aaagccggag aaacgcccca gccccatgcg gctaccgaca gacgaaagta
    12421 accc
//
LOCUS       DAZKJR010000145        12297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKJR010000145 DAZKJR010000000
VERSION     DAZKJR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.146"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(62..343)
                     /gene="yehE"
                     /locus_tag="ACHOWS_004209"
     CDS             complement(62..343)
                     /gene="yehE"
                     /locus_tag="ACHOWS_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFQ0049702.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHOWS_004210"
     CDS             complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHOWS_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFQ0049703.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPNMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1847..3880
                     /gene="metG"
                     /locus_tag="ACHOWS_004211"
     CDS             1847..3880
                     /gene="metG"
                     /locus_tag="ACHOWS_004211"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0049704.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEEIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4022..7810
                     /locus_tag="ACHOWS_004212"
     CDS             4022..7810
                     /locus_tag="ACHOWS_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFQ0049705.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDALDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDELQANNAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            7820..11452
                     /gene="yehI"
                     /locus_tag="ACHOWS_004213"
     CDS             7820..11452
                     /gene="yehI"
                     /locus_tag="ACHOWS_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFQ0049706.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQHEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQARAVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALPFWLRINEKKHLSAGEDYFLSIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPA
                     RMPKAPDFWHPLMWSRPRLIINHQLITDDALEIIGEMLRFTQGGRFHSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSTR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRTDDDQLKAPE
                     ALARLKGLKKDTTQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTEGMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYGLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11514..11867
                     /locus_tag="ACHOWS_004214"
     CDS             11514..11867
                     /locus_tag="ACHOWS_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049707.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRKLQNIFNQ
                     LIEYYFIKGGSGFPP"
BASE COUNT         3208 a         3145 c         2982 g         2962 t
ORIGIN      
        1 cttaaatcat tcatgtgaat gatttaagct ttctaaatcc gattaaaacc agggtggcaa
       61 attaatcgta gcgatttaac acgattcgac gtttgctatc gctatctgca gcaatcactt
      121 ccgtcgttac acctttaact ggcgaagaaa cctgatgacg ggtatcaaca ttatacgtta
      181 actgaccaca atgctgttga ggttgaccat taaccatagc catactgcat gtcgggggac
      241 tgatcctacc attaatcgtt aatgtggcag tttctgaaga gaacgcggga gaggccaatc
      301 cataggcaag aaaaataatt cctgacagcc aatacttatt cattgaacgt tatccctgta
      361 gtaaaggtta tgcctggata gaatgagtgc ataacaaact atagctgtac atccactaca
      421 cagccacgaa ggatgataat gaagcattgc ctgtacgatc aatcgacttt gtagagtttc
      481 ggacgaaggt ccgcagaata ttcgcagcat taaataagtg ttcattaatg cggcaaaaac
      541 aaatacaccg catcaatgta acatctctgc ggtgtatgaa gaataaccag aatggttaat
      601 acgcgttaga ccgcgcggaa ggaaatctcg cctggaatga cttcaccctg ccagtaaagc
      661 tgagctgcaa cgcggtcagc cagctggcgg tagatagcgg taaattcgct ctcaggacgg
      721 ctgataaccg ttggcgtgcc tttatcgaga tcctcacgta aggagatgtg taacggcatc
      781 tgccccagca gctgtgtgtg gtatttctcg gccagtttct ctgcaccacc ggtaccgaaa
      841 atcggttcat ggtgaccaca gttactgcaa atatgcacac tcatgttctc gacaataccg
      901 agcaccggaa cctcgacttt ctcgaacatc acaatgcctt ttttcgcatc gatcaacgcg
      961 atgtcctgcg gggtggtgac cacgactgca ccggttactg gaatgttttg tgccaatgtc
     1021 aattgaatgt caccggtacc tggcggcata tcaagcacca gatagtcgag atccggccac
     1081 aaggtttcct gcaacatttg cattaacgcc ttgctcgcca ttgggccacg ccacaccatc
     1141 gcattgtcgt ctgtcaccag ataaccaatt gaattagttg ccaggccatg agacataatc
     1201 ggtgccatat gggtaccgtc cggcgaagtt ggacgctgat tttctgcacc cagcatgtta
     1261 gggattgatg ggccgtagat atcggcgtcc agaatgccga ctttcgcccc ttccgccgcc
     1321 agtgccaacg ccaggtttac cgccgtggag gatttaccca cgccaccttt accggaactg
     1381 accgcgataa tgttcttcac gccgttaatt cccggctgat tcttcacgcg tttcagagtg
     1441 gctatgttat gcgacagttt ccagtcgata gctttcgctc cggtgatgcg cagcagttcg
     1501 gcgctacatt gctcttttaa ctcttcgaac gcgctatgcc agacgaatgg cataaccagt
     1561 tccacatgca gcgtgtcgtc catccaggcg acatggtgca acgctttcag cgttgtgagg
     1621 ttatgcttca gggttgggtg ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag
     1681 gcttctggtg atttggcctg ggattgttcg ttcatcccga ctcctttttt gtatagataa
     1741 accatcagtt gatagtttac ctgaagaata tagagaagta cttacttaac attttcccat
     1801 ttggtactat ctaagccctt ttcactatta agaagtaatg cctactatga ctcaagtcgc
     1861 gaagaaaatt ctggtgacgt gcgcactgcc gtacgctaac ggctcaatcc acctcggcca
     1921 tatgctggag cacatccagg ctgatgtctg ggtccgttac cagcgaatgc gcggccacga
     1981 ggtcaacttc atctgcgccg acgatgccca cggtacacca atcatgttga aagctcagca
     2041 gcttggtatc actccggagc agatgattgg cgaaatgagt caggagcatc agactgattt
     2101 cgcaggcttt aacatcagct atgacaacta tcactcgacg cacagcgaag agaaccgcca
     2161 gttgtcagaa cttatctact ctcgcctgaa agaaaacggt tttattaaaa accgcaccat
     2221 ctctcagctg tacgatccgg aaaaaggcat gttcctgccg gaccgttttg taaaaggcac
     2281 ctgcccgaaa tgtaaatccc cggatcaata cggcgataac tgcgaagtct gcggcgcgac
     2341 ctacagcccg actgaactga tcgagccgaa atcggtggtt tctggcgcta ccccggtaat
     2401 gcgtgattct gaacacttct tctttgatct gccctctttc agcgaaatgc tgcaggcatg
     2461 gacccgcagc ggtgcgttgc aggagcaggt ggcaaataaa atgcaggagt ggtttgaatc
     2521 tggcctgcaa cagtgggata tctcccgcga cgccccttat ttcggttttg aaattccgaa
     2581 cgcgccgggc aaatatttct acgtctggct ggacgcaccg attggctaca tgggttcttt
     2641 caagaatctg tgtgacaagc gcggcgacag cgtaagcttc gatgaatact ggaagaaaga
     2701 ctccactgcc gagctgtacc acttcatcgg taaagatatt gtttacttcc acagcctgtt
     2761 ctggcctgcc atgttggaag gtagcaactt ccgcaagccg accaacctgt ttgttcacgg
     2821 ctatgtaacg gtgaacggcg cgaagatgtc caagtctcgc ggcaccttta ttaaagccag
     2881 cacctggctg aatcattttg acgctgacag cctgcgttac tactacactg cgaaactctc
     2941 ttcgcgcatt gatgatatcg atctcaacct cgaggatttc gttcagcgtg tgaatgctga
     3001 tatcgtcaac aaagtggtta atctggcgtc ccgtaacgcg ggctttatca acaaacgttt
     3061 tgacggcgtg ctggcaagcg aactggctga cccgcaactg tacaaaacct tcactgatgc
     3121 cgctgaagag attggcgaag cgtgggaaag ccgtgaattt ggtaaagcag tgcgcgaaat
     3181 catggcactg gctgacctgg ctaaccgcta tgtcgatgaa caggctccgt gggtggtggc
     3241 gaagcaggaa ggtcgcgatg ctgacctgca ggcgatttgc tctatgggca ttaacctgtt
     3301 ccgcgtactg atgacttacc tgaagccggt actgccgaaa ctgactgaac gagcagaagc
     3361 attcctgaat acggaactga cctgggatgg tatccagcaa ccgctgctgg gccataaagt
     3421 gaatccgttc aaagcactgt ataaccgtat cgacatgaag caggttgaag cactggtgga
     3481 agcgtctaaa gaagaagtga aagccactac cgcgccagtt actggcccac tggcagatga
     3541 tccgattcag gaaaccatca cctttgacga cttcgctaaa gttgacctgc gcgtggcgct
     3601 gattgaaaac gcagagtttg ttgaaggttc tgacaaactg ctgcgcctga cgctggatct
     3661 cggcggtgaa aaacgcaatg tcttctccgg cattcgttct gcttacccgg atccgcaggc
     3721 acttattggt cgtcacacca ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg
     3781 tatctccgaa ggcatggtga tggctgctgg tcctggcggg aaagatatct tcctgctaag
     3841 cccggatgcc ggtgctaaac cgggtcatca ggtgaaataa tccccccttc aaggcgctgc
     3901 atcgacagcg ccttttcttt ataaattcct aaatttgttt tcttgcgatt ttgtctctct
     3961 ctaacccgca taaatactgg tagcatctgc attcaactgg ataaaattac agggatgcag
     4021 aatgagacac tttatctatc aggacgaaaa atcacataaa ttctgggcgg tggagcagca
     4081 gggaaacgaa ctgcacatca actggggtaa agtcggcacc aacggacaaa gccaggtaaa
     4141 aagttttgcg gatgcagcag cggcggaaaa agcgggactg aagctgattg cagagaaggt
     4201 gaagaagggg tatgtggagc aagcgaagga taattctttg caaccttccc aaacggtaac
     4261 ggactctctc aaggtagctg acttatccac cattattcag gaacaaccct ctttcgtagc
     4321 agaaacccgt gcggctgaca aaaatacaga tgctgtttta ccgtggctgg cgaaagatat
     4381 tgctgtcgtt tttccgcccg aagttgtaca caccacgtta agtcatcgcc gctttcccgg
     4441 agttcctgtt cagcaagcag acaaattgac ccaattacgt cgcttagcct gtagtgtgtc
     4501 gcaacgggat aataaaacag ccacatttga cttcagcgcc tgttctttag aatggcaaaa
     4561 caccgtcgcc caggcgatca gtcagatcga cggcctgaaa acaacacagc taccatcacc
     4621 cgtaatggct gtactcactg cacttgaaat gaaatgcaca agatataaag agcgtgagga
     4681 tgttatggat cagattattc aggaaggcgg tctggaatat gctactgatg taataataca
     4741 tcttcaacaa atatctattg aatgggatta tgtcaacaat aatattgttt tcctgtcgtc
     4801 tggcatttca cctgattact tgcagcaata ttccagcttt gaattacgcc tacgtaaaca
     4861 tttatcactg gcggaagagt ctctctggca aaaatgtgca caaaaactta ttgccgcaat
     4921 tccacatatt ccagaatggc ggcaaccatt aattgctttg ttattacccg aaaaaccaga
     4981 aattgcacat gaaatttccc agcgtctact ggggcaaaag aaattaccct cgcttgagtg
     5041 gctaaaaata gtggcgactg atgagcacat tcttgcctca ttagaaaaat atcacgagcc
     5101 atatgccatt tttgatgatt actattgtgg tgcgatatgg tcagccaccg tattacagga
     5161 gcaaggtgtt acagccctgc cccgatttgc tccctatgcc gcaagtgact actgcgccga
     5221 tgtgttgcgt catatcaatc atccgttcgc attaacactg cttatacgtg tagccgggca
     5281 tactaaacgc tgtcacgatc gaatgacgaa agcctgcgct gcgttcccac acgcagcact
     5341 ggcagcactg gcggaacttc tagtgcaaaa agaagagaat agttggcgca ttatgctaat
     5401 gacgatgctt atctcgcatc cgacactagc agaacaggtc attccctggc tttcgacacc
     5461 cgcagttgca gtgctgaaat catgtcagca acaactgaca cagccctcaa accatgccag
     5521 cgccgatcta ctgccggcca tagtagtctc cccaccatgg ctttcgaaaa agaaaaaatc
     5581 gccgattccg gtgctggatt tagcaccgct caacctcgaa tccatatgca caataaccga
     5641 cacagaagct aaagagtttc aaactcattg ggactgggaa ccgcataagc caagtgaagg
     5701 tgctaaaaat ttcctttact cgctggggta tcgccgctgg gattttgaca catataaata
     5761 tatcggcgct tctgactcgg caattgacgc atgggaacgt gaggacttcg ctacactcat
     5821 ccaaatgttt aaagcccacc atgcaccgta tcagggggag tggcatctca actcacttcc
     5881 ctttttacct atgcaaaaag cgattaagct atgggaattt ctcagcaaag agccgcatac
     5941 cgcgataaaa ccagtcatgc tttatttgcg actggcaggt atgagtggtt ttctacactc
     6001 gttttcacgc taccctcaag aaggttttgc ggttgctaat tattttgccg caacggaact
     6061 ggctcctgcc gtcgcccgcg ccttcaacaa actcaaaaca ttacgacaag acgccagtag
     6121 ctggctgctg aaatacccgg aacacgccat taccggcctg ctgcctgcag cgctcggcaa
     6181 agccagtgaa gcacaggaca acgcccgtgc tgccttgcgt atgcttaccg aaaacggtca
     6241 tcagccattg ctgcaagaaa tcgcccggcg ttataaccag ccggaagtaa ccgatgcggt
     6301 gaacgctcta cttgcgctcg atgccttaga taatcacccg acaaaaatcc ccactcttcc
     6361 gaccttttat cagccatcgc tctggacgcg cccgttatta aaagcaaacg cccaatcact
     6421 gcctgatagc gcactcctcc acctcggtga aatgctccgc ttccctcagg aagaggcact
     6481 gtatccggga ttattgcagg tgaaagatgc ctgtaccacc gactcactgg ctgaatttgc
     6541 ctgggatctg tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt
     6601 cactgcgtta ggcgttctcg gtaacgatga caccgcccgc aaactgacgc ctttgattcg
     6661 cgcctggcct ggtgaatccc agcataaacg cgccaccgtc gggttggata ttctcgctgc
     6721 tatcggtagt gatatcgccc tgatgcagct taacggcatc gcccagaaac tgaaattcaa
     6781 agcattacag gagcgggcaa aagaaaaaat tgccgacatt gccgagagcc gcgaactcac
     6841 ggtggcggag cttgaagatc ggttagcacc ggatctcggt ctggatgata atggttcgtt
     6901 gttgctggat ttcggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt
     6961 tgtgcgtgac gcttccggca gtcgtctgaa agacctgccc aaaccaaaca aaagcgatga
     7021 tgaattacag gcgaacaatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac
     7081 cgtcgccgcc cagcaggtag caaggctgga atccgccatg tgcctgcgtc gccgctggtc
     7141 accggaaaac tttcagctct tcctggttga gcatccgctg gtccgccact taacccgccg
     7201 tctgatttgg ggcgtttata gcgccgaaaa ccagctactg gcttgctttc gcgtagcgga
     7261 agataacagc tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc
     7321 tattggtatt cctcacgttc tggaaatatc accgacggat gctgccgcct tcgggcagct
     7381 ttttgccgac tacgaactgt taccaccgtt ccgccagctt gatcgtaaca gttacgcgct
     7441 gacagaagcc gagcgcaatg ccagcgaact gacacgctgg gcaggcagaa aatgcccgag
     7501 tggtcgggta atggggctgg caaataaagg ctggatgcgt ggtgaaccgc aggacggtgg
     7561 ctggatcggc tggatgatca aacctctggg ccgctggtcg ttaatcatgg aaatcgatga
     7621 aggttttgcg gtcggcatgt cgccagccga actcagtgcc gagcaactct taagcaagct
     7681 gtggctatgg gaaggcaaag cggaaagcta tggctggggg agtaattcaa cacaggaagc
     7741 gcagttctcc gtactcgatg ccatcaccgc cagcgagcta attaacgata ttgaagccct
     7801 gtttgaataa ggaaagagca tggacaagga attaccgtgg ctggcggata acgcccaact
     7861 ggaactgaaa tataaaaaag gcaaaacacc actcagccat cgcaattggc caggcgaacc
     7921 ggtacctgtt atcactgaaa acatcattca gacactgggt gatgaattgc tacaaaaagc
     7981 agagaagaaa aaaaacattg tctggcgtta tgaaaacttt tcactggagt ggcagtccgc
     8041 catcacgcag gccatcaact tgatcggcga acacaaaccc tcaatcacgg cccggacaat
     8101 ggcggcgcta gcctgtatcg cgcaaaatga tagccaacag ttgctcgacg aaatcgtcca
     8161 acacgagggg ctggaatatg caactgaggt ggtgattgca cgccagttta ttgtacggtg
     8221 ttatgagagt gatcctctgg tagtaacatt gcaatatcag aacgaggact atggctatgg
     8281 ttatcgctca gaaacctata acgaattcga tctccgattg cgtaagcatc tctctctggc
     8341 agaggaaagc tgctggcagc gttgcgccga caaactcatt gccgcactgc caggaataac
     8401 caaagttcgc cgccctttta ttgcgctgaa cctcccggaa aaacccgaaa tcgccaacga
     8461 gctggtaagc cttgaatgtt cgcaaactca ttttcgctca aaagagtggt taaaagttgt
     8521 tgctgatgac cctaaggcgg tgaaagaact cgcacgttac tggagccagg atatatttag
     8581 cgatcgagaa gccagctaca tgtcgcatga aaaccacttc ggctacgcgg cctgcgctgc
     8641 ccttttgcgc gaacaaggac tggcagccgt tccgcgcctc gcgatgtatg cacataaaga
     8701 agattgcggc agtctgctgg tacaaattaa ccatccgcaa gtcatccgca ccttgctact
     8761 ggtggctgat aaaaacaaac ccagcctgca acgtgtggct aaatacagta aaaacttccc
     8821 ccatgcgacg ctcgccgcac tggcagaact gctggcgtta aaagcccccc ctgcgcgccc
     8881 tggttatccc atcatcgaag accaaaagct gcctgcacag caaaaagcac gtgatgaata
     8941 ctggcatacg ctgttacaaa cgctgatggc atcgcagcca caactggcag aagaggtgat
     9001 gccgtggtta agtactcaag cccgggcagt gctgaatagt tatttatcgg caccgcccaa
     9061 aacggttatt gatagcaccg ataacagcca aatgcctgaa atcctcgttt caccaccgtg
     9121 gcgtagtaag aaaaaaatga cagctccacg tcttgatttg gcaccgctcg aattaacccc
     9181 gcaagtttac tggcaaccag gcgaacaaga gaggcttgcc tccactgagt ctgcccgtta
     9241 tttcagcacg gaatctcttg ctgaacgcat ggaacaaaaa agtggacgag ttgtattaca
     9301 ggaactgggt tttggggatg atgtatggct gtttctgaat tatatactcc ccggaaaact
     9361 ggatgctgca cgcaattcac tcattgttca gtggcattac tacccagggc gggttgaaga
     9421 gatcatgaat ggctggagct ccccggaagc tcaattagca gaacaggcgc ttcgcagcgg
     9481 tcacgtagaa gtgttaatta acatatggga aaatgacagc tactcacgtt atcgtccgga
     9541 aaagagtgtc tggaacctgt atttattggc acagttgccg cgtgagatgg ctttgccttt
     9601 ctggctacgt atcaatgaga aaaagcatct gtctgcgggt gaggactatt ttctcagtat
     9661 cttcggattg gatgctctac caggtctgct gttggctttt tcacatcgtc caaaagaaac
     9721 atttccgtta attttaaatt ttggcgcaac agaactggcc ctgcccgttg cccgcgtctg
     9781 gcgacgtttc gcggcgcagc gtgatctggc ccgccagtgg attttacaat ggccggaaca
     9841 tacggctact gcacttatcc ctcttgtctt caccaaaccc agcgataata gcgaagctgc
     9901 attacttgcc ctacgtttac tttacgaaca gggacatggt gaattgctgc aaaccgcggc
     9961 aaaccgctgg cagcgtacag atgtatggcc tgccctggag catttactta aacagagtcc
    10021 aatagagatt tacccggcac gcatgccaaa agcccctgat ttctggcatc cgctaatgtg
    10081 gtccaggccg cgcctaatca ttaatcatca acttattacc gatgacgctc tggaaattat
    10141 cggcgaaatg ctgcgcttta cccagggggg acgttttcat agcgggctgg aacaactgaa
    10201 aacgttctgc cagccgcaaa cgctggcagc ttttgcctgg gatctcttca ctgcgtggca
    10261 acaagctggt gcccccgcaa aagacaactg ggcatttctg gcgttaagtc tctttggtga
    10321 cgaaagcacg gcacgggatc taacgacaca gatcctcgcc tggccacaag aaggcaaatc
    10381 tacccgtgct gtcagtggcc tgaacatcct taccctgatg aataatgata tggcgctgat
    10441 acagctgcat catatatcgc aacgggcgaa atcccgcccc ttacgtgata acgcggcgga
    10501 atttcttcag gtggtcgcag aaaatcgcgg gctaagccag gaagagttag cggacagatt
    10561 agtcccaaca ctgggccttg atgatccgca ggcgttgagt tttgattttg gtccccggca
    10621 gtttaccgtt cgcttcgatg aaaaccttaa cccggttatc tttgatcagc aaaacgttcg
    10681 ccagaaaagc gttccccggt tgcgcaccga tgacgatcaa ctgaaagcgc ccgaggcact
    10741 ggcccgatta aaagggctaa aaaaagatac gactcaggtg agcaaaaacc tgctcccccg
    10801 tcttgaagct gccctgcgta ccacccgacg ctggtcgctg gcagattttc attctctgtt
    10861 tgttaatcat ccctttaccc gtctggttac ccagcgatta atatggggcg tgtatccggc
    10921 aaatgaaccg cgtcgtttac tcaacgcctt tcgtgtggcc gcagaggggg agttctgcaa
    10981 tgcgcaagat gagcccattg acctgcctgc ggatgctctg attggcattg cccacccgtt
    11041 agaaatgaca gaaggaatgc gcagtgaatt tgcacagctt tttgccgatt acgaaattat
    11101 gccgcctttt cgccagttgg cgcggcgcac ggtgctgctc acacctgacg agtcaaccag
    11161 taacagcctg actcgctggg aaggtaaatc cgctaccgtt gggcagctta tgggaatgcg
    11221 atacaaaggc tgggagtcag gctatgagga cgcatttgtc tatggcctgg gcgagtaccg
    11281 gctggtcctt aagttttcac ccggttttaa ccactacaat gttgatagca aagcgttaat
    11341 gagcttccgt tctcttcgag tgtaccgtga caataaatcc gtcacttttg ccgaacttga
    11401 tgtgtttgat ttgagtgagg cgttaagcgc acctgacgtc attttccatt aaaaacacag
    11461 cgggcagtga tgcaactgcc cgttatcaac acgacttacc agcggggaaa gcgatgatcg
    11521 tgcaaaaaga gctggttgct atttacgatt atgaggtccc tgtacctgaa gatccgtttt
    11581 ccttcagact tgagatccat aaatgctctg aattatttac aggttccgtc tatcgactgg
    11641 agcgattccg gctacgtcca acatttcatc aacgtgatcg agaagatgct gacccgctaa
    11701 taaatgatgc gttgatttat ataagagatg agtgtattga tgagcggaaa ttacgaggtg
    11761 aatcacctga aactgtaata gcaattttta atcgtaaact acagaatata ttcaaccaac
    11821 taatagaata ttactttatt aaagggggaa gcggttttcc cccttagttg cgtttcaatt
    11881 tgcccattac ttcaacaaat caatcaaccg taacagagcc actaatagct ccagtacagc
    11941 aatgagcacg gttaactttt gtgtcagcgt gtccatccgc tctccttatt aaggagcgca
    12001 acaccagcac cttaccctta cactgcctgt cgacactggt ttacgtttgt tgagtcgcca
    12061 tgttgcactc cggatcagag cactacctgc ggcaccaccc gtccggtagc caggacttga
    12121 aatgggttcg atccggcgca acttcccaac agtcaggatt ataaatctca ttatcttaat
    12181 tgcaacacgg tccagcggtg gttataattc gttcgttgag atgccatgtt gcactatcag
    12241 taacttaaca tcaaagtagt aaataaaaga acctgcctca ccagcaggtt cttttat
//
LOCUS       DAZKJR010000146        12107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKJR010000146 DAZKJR010000000
VERSION     DAZKJR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.147"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..682
                     /gene="yebB"
                     /locus_tag="ACHOWS_004215"
     CDS             80..682
                     /gene="yebB"
                     /locus_tag="ACHOWS_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFQ0049708.1"
                     /translation="MNVNYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(684..1205)
                     /gene="ruvC"
                     /locus_tag="ACHOWS_004216"
     CDS             complement(684..1205)
                     /gene="ruvC"
                     /locus_tag="ACHOWS_004216"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFQ0049709.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1240..1980)
                     /gene="yebC"
                     /locus_tag="ACHOWS_004217"
     CDS             complement(1240..1980)
                     /gene="yebC"
                     /locus_tag="ACHOWS_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0049710.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2009..2461)
                     /gene="nudB"
                     /locus_tag="ACHOWS_004218"
     CDS             complement(2009..2461)
                     /gene="nudB"
                     /locus_tag="ACHOWS_004218"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFQ0049711.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2579..4351)
                     /gene="aspS"
                     /locus_tag="ACHOWS_004219"
     CDS             complement(2579..4351)
                     /gene="aspS"
                     /locus_tag="ACHOWS_004219"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0049712.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKDENN"
     gene            4661..5227
                     /gene="yecD"
                     /locus_tag="ACHOWS_004220"
     CDS             4661..5227
                     /gene="yecD"
                     /locus_tag="ACHOWS_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0049713.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5224..6042
                     /gene="yecE"
                     /locus_tag="ACHOWS_004221"
     CDS             5224..6042
                     /gene="yecE"
                     /locus_tag="ACHOWS_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0049714.1"
                     /translation="MIYIGLPQWSHPKWVRLGITNLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6095..6490
                     /gene="yecN"
                     /locus_tag="ACHOWS_004222"
     CDS             6095..6490
                     /gene="yecN"
                     /locus_tag="ACHOWS_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFQ0049715.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6531..7274
                     /gene="cmoA"
                     /locus_tag="ACHOWS_004223"
     CDS             6531..7274
                     /gene="cmoA"
                     /locus_tag="ACHOWS_004223"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFQ0049716.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7271..8242
                     /gene="cmoB"
                     /locus_tag="ACHOWS_004224"
     CDS             7271..8242
                     /gene="cmoB"
                     /locus_tag="ACHOWS_004224"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFQ0049717.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8407..10836)
                     /gene="torZ"
                     /locus_tag="ACHOWS_004225"
     CDS             complement(8407..10836)
                     /gene="torZ"
                     /locus_tag="ACHOWS_004225"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFQ0049718.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10861..11961)
                     /gene="torY"
                     /locus_tag="ACHOWS_004226"
     CDS             complement(10861..11961)
                     /gene="torY"
                     /locus_tag="ACHOWS_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFQ0049719.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2788 a         3198 c         2945 g         3176 t
ORIGIN      
        1 aatacacatc gcgacccctg aaaaaataaa ttttttatgc tattacgtat attcatatct
       61 atttcaatgg aatgacaacg tgaatgttaa ttatcctgct gaatatgaaa ttggtgatat
      121 cgtctttaca tgtataggtg ctaccttatt tggtcaaata tcagctgcat caaattgctg
      181 gagtaatcac gtcgggatca ttatcggtca taacggtgaa gacttcctgg ttgcagaaag
      241 ccgggttccc ctttcaacca tcaccacgct atctcgtttt attaaacgtt cgtctaatca
      301 acgctatgct ataaagcgat tagacgctgg gctgacagaa caacaaaaac aacgaattgt
      361 tgaacaggtt ccctcccggc tacgcaaact ttaccacacc ggttttaaat acgaatcttc
      421 gcgccagttc tgttcaaaat ttgtttttga tatttataaa gaagcgctat gtattccggt
      481 gggtgaaata gagacgtttg gagaattgtt aaatagcaat ccgaatgcaa aactcacttt
      541 ctggaaattc tggttcttag gttctattcc gtgggagcgt aaaaccgtca cgccagccag
      601 tttgtggcat catccgggtt tggtgttgat tcatgcggag ggagtggaaa cgcctcagcc
      661 ggaactgacc gaggcggtat aacttaacgc agtcgccctc tcgccaggtt cagccgcgat
      721 tcgctcatct gcatcgcatt ctgactgacg tgacaatggg taatcgcgat agctaatgca
      781 tcggcagcat ccgcctgtgg attagcgggc agtttcagca aggtgcggac catatgctgc
      841 acctggcttt tttcggcact accaatacct accaccgttt gctttacctg acgtgccgca
      901 tattcaaata ccggcaattc ctgattcacc gctgccacaa tcgccacgcc gcgcgcctga
      961 cccagtttca gggctgagtc agcgttcttc gccataaaga cttgttcaat ggcgaaataa
     1021 tcaggctgga actgggtgat gatttccgtc acgcccgcat agatgagctt cagacgagac
     1081 ggtaaatcat ccactttggt gcgtatgcat ccgctaccca ggtaggacag ttgcctgcct
     1141 acctggcgga tgacgccata gccggtcacg cgcgaacccg ggtcaatgcc gagaataata
     1201 gccatcacgc gtctccgttt tgctgtttag caggcctcat cagagagtcg ctgcaacctc
     1261 atcagagatt tcaccgttat ggtaaacttc ctgcacgtcg tcgcaatctt ccagcatatc
     1321 gatcagacgc atcagtttcg gtgcggtttc tgcatccata tcagctttgg tggacgggat
     1381 catggaaact tccgcgctgt ctgctttcag acctgccgct tccagagcgt cgcgtacttt
     1441 gcccatttct tcccatgcag tgtagacatc aatcgcgccg tcatcatagg tcacaacgtc
     1501 ttcagcaccg gcttccaggg ctgcttccat gatggtgtct tcatcgcctt tctcgaagga
     1561 gatcacgcct tttttgctga acagataagc tacggaacca tcagtaccga ggttaccgcc
     1621 acatttactg aatgcatggc gcacttcagc aacggtacgg ttgcggttgt cagacagaca
     1681 ttcaatcatg attgccgtgc cgccaggacc gtaaccttcg tagatgatgg tttccatgtt
     1741 tgcatcatca tcaccgccca cacctcgtgc aattgcgcgg ttcagagtgt cacgggtcat
     1801 gttgttagac agtgctttat caattgctgc acgcagacgc gggttagcgt ccggatcacc
     1861 accgcccagc ttagccgcgg ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg
     1921 cttagcatcc tgcgcagctt tacgatgtct ggtgttggcc catttactat gacctgccat
     1981 aaaaatatct ccagatagcc ctgcctgttc aggcagcgtt aattacaaac tgttcaatcg
     2041 cctgccggtt gctccaggac ttagtgagcg ccgcagcagc aggcgcgtca agccacttgt
     2101 aagccagatg ttcagtgaaa acgatctggc gctcgtgcgg gagcgcaaga cagaaccatg
     2161 attccgtatt acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa
     2221 actctaccgt gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga
     2281 cctcttcctt tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc
     2341 cggttaccga ctgccagaaa tcgggatcgt cacgccgctg caacatcagc acccgtttcg
     2401 tatcttgtgc atagatgacc actaagatcg aaacgggacg cttatacact ttatccttca
     2461 cgctgcctct tcgttgactg ccttcgctca tcccattcac atagttatct atgctcatgg
     2521 gagttcactc agttgccgcc tcgatgcaac gcgaatgatt tcgtgtattt gagtcatatc
     2581 agttattctc gtccttcttc acaacctgaa tgctcagctc agccagtgca gtcgggttag
     2641 caaagctcgg tgcttcagtc atcagacacg ctgccgccgt ggttttcggg aaggcgataa
     2701 cgtcacggat attgtcggtg ccggtcagca gcatggtcag acggtcaaga ccgaatgcca
     2761 gacctgcgtg cggcggagta ccgtatttca gggcgtcgag caggaagccg aatttctcgc
     2821 gctgttcctc ttcgttgata cccagaatac caaacaccgt ctgctgcata tcaccattat
     2881 ggatacgcac agaaccaccg cccacttcgt aaccattgat gaccatatcg taagcgttcg
     2941 ccaccgcatt ttctggtgca gctttcagtt ctgcagcagt catgtctttc ggtgaggtga
     3001 acggatggtg cattgccgtc aggccgcctt caccgtcgtc ttcaaacatc gggaagtcga
     3061 taacccacag cggtgcccat ttgctttcgt cggtcagacc aaggtcttta cccactttca
     3121 ggcgcagtgc gcccatcgcg tcggcaacaa ttttcttgtt gtcggcaccg aagaaaatca
     3181 tatcgccatc ttgcgcggca gtacgatcca ggatggcttc gatgatttct gcattaagga
     3241 acttcgctac cgggctgttg ataccttcca gacctttcgc gcgttcgtta actttgatgt
     3301 aagccagacc tttcgcgccg tagattttaa cgaagttacc gtattcgtcg atctgcttac
     3361 gggtcaacga tgcgccgccc ggaacacgca gagcggcaac acggcctttc ggatcgttcg
     3421 ccggacctgc aaatactgca aactcaacag atttcagcag atcggcaacg tcggtcagtt
     3481 ccatcgggtt acgcagatcc ggtttatcag aaccataacg gcgttctgct tctgcaaagg
     3541 tcattaccgg gaaatcgccc agatccacgc ccttcacttc cagccacaga tgacgcacca
     3601 gcgcttccat cacttcacgc acttgcggcg cggtcatgaa agaagtttcc acatcgatct
     3661 gagtaaattc aggctgacgg tcagcacgca ggtcttcgtc acggaagcat ttaacgatct
     3721 gatagtagcg gtcaaagccg gacatcatca gtagctgttt gaacaactgc ggggattgcg
     3781 gcagcgcgta gaatttacct ttgtgcacac gagaaggcac caggtagtca cgcgcgcctt
     3841 ccggcgtggc tttggtcagc atcggagttt cgatgtcgag gaagccgtgg tcatccataa
     3901 aacggcgcac caggctggtg attttagcgc gggttttcag gcgctgagcc atttccgggc
     3961 gacgcaggtc gaggtagcgg tatttcagac gcgcttcttc ggtgttgacg tggttagagt
     4021 caagcggcag aacatctgca cgattgatga tagtcagcga ggacgccagt acttcgattt
     4081 cgccagtcgc catatcgcgg ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtga
     4141 cctgaatgca gaactcatta cgcagttcag aggccagctt taacgcgtcc gcacgatccg
     4201 gatcgaaaaa tacctgcacg ataccttcgc ggtcgcgcat atcgatgaag atcaggctac
     4261 caagatcacg acgacggttg acccaaccac acagagtcac ctgctgcccc acgtgggaca
     4321 aacggagctg tccacaatat tctgtacgca tgagatatcc cttaacttag ctgccggcgg
     4381 atgccccctg ctgcgcaggt gaccaagtcg cagcgttagc tgtatgtcac aactgaatga
     4441 aaaaaggcgg ctattatact ggaaattctg ccgcaccgta agagcctggc ccgcgctgga
     4501 acgcctcgtt accactttat atcgggcctg aaatcagact ctacgccagt ttgctataaa
     4561 ggtgttgccc gaactcataa aaattaacaa aatttgtcgt tccgccatcg gctaatcgca
     4621 ttaaggtgag aggcacgatt ttgttttgtc aggagtcatc atgcttgaac ttaatgctaa
     4681 aaccaccgcg ctggtggtga ttgatttaca agaaggcatc ttaccttttg ccggaggtcc
     4741 acatactgcc gatgaggtgg ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgccag
     4801 cggtcagccc gtgtttctgg tgcgcgttgg ctggtctgcc gattacgccg aagcattaaa
     4861 acagccggtt gatgccccct cccccgcaaa agtgttgccc gaaaactggt ggcaacatcc
     4921 tgctgcatta ggtgcaaccg acagcgatat cgaaatcatc aaacgtcaat ggggtgcgtt
     4981 ttacggtacg gatctggagt tgcaattacg ccgccggggt atcgatacaa tagtgttatg
     5041 tgggatctcg accaatatcg gtgttgaatc caccgcccgc aatgcctggg aactcggttt
     5101 taatctggtg attgccgaag atgcctgtag cgccgccagc gccgagcagc acaataacag
     5161 cattaatcat atctacccgc gcatcgcccg tgtgcgcagt gtggaagaga tcctccacgc
     5221 gttatgattt acatcggtct gccgcaatgg tcgcatccta aatgggtgcg gttggggatc
     5281 accaaccttg aagagtatgc ccgccacttt aactgcgtgg agggtaacac cacgctttat
     5341 gccctgccga aacccgaggt tgtcctgcgc tggcgtgagc agaccacaga tgacttccgc
     5401 ttctgtttta agtttccggc gaccatttcg catcaggcag cattacggca ttgcgatgat
     5461 ttagtgactg aatttttgac ccgcatgtca ccgttggctc cgcgcattgg acaatactgg
     5521 ctgcaactgc ctgccacatt cggcccacga gagctgcctg cgctttggca ttttctcgat
     5581 tctcttcccg gtgaatttaa ttatggggtg gaagtccgcc atccacagtt tttcgccaaa
     5641 ggggaagagg aacaaacgct taatcgcggt ttacatcagc gcggcgttaa tcgggtgatt
     5701 ttagacagcc gcccggttca tgcagcacgt ccacacagtg aagctattcg cgacgctcaa
     5761 cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg cgaaaaatcc actgatccgt
     5821 tttatcggta gtgatgatat gacgcaaaac cgggagttat ttcaggtctg gttacaaaaa
     5881 ttagcacagt ggcatcagac cactacgcct tatctttttt tacatacgcc agatattgcc
     5941 caggccccgg aactggtaca taccctgtgg gaagacttac gtaaaacgct tccagagatc
     6001 ggagcagttc cggctattcc acagcaatct tctcttttct gaatttgcca cctatcatag
     6061 acaggtgcca tcggccattt taaagggagt ttgtatggta agcgcgctgt atgccgtttt
     6121 aagtgcgttg ttattaatga agttctcttt tgatgtcgtt cgcctgcgaa tgcagtaccg
     6181 cgttgcctat ggcgacggcg gttttagcga actgcaaagc gctattcgca ttcatggtaa
     6241 cgcggtggaa tatattccta tcgcgattgt gttgatgctg tttatggaaa tgaatggcgc
     6301 agaaacctgg atggtgcata tttgcggcat cgttttgctt gctggtcgtc tgatgcatta
     6361 ttacggtttt catcaccgtc tgttccgctg gcgacgttct ggcatgagcg cgacctggtg
     6421 tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg ccctgggagt tggttttctc
     6481 cctgcgttag cgcacaatac gccactttct ttttcccgga tttttacgtt atgtctcacc
     6541 gcgacacgct attttctgcc cctatcgcca gactgggcga ctggaccttt gatgaacggg
     6601 tagctgaagt cttcccggat atgatccagc gttccgttcc cggctattcc aatattattt
     6661 ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg
     6721 gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa cattcatcat gataattgca
     6781 aaattattgc catcgataac tcccccgcga tgattgaacg ctgccgtcgt catattgacg
     6841 cctataaagc ccctacgcca gtagacgtca ttgaaggtga tattcgcgat atcgccattg
     6901 aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt cctggaacct tccgagcgcc
     6961 aggcgttact ggataaaatt tatcaagggc tgaacccggg cggcgcgctg gtgctttcgg
     7021 aaaaattcag tttcgaagat gccaaagttg gtgaactgtt gttcaacatg caccacgact
     7081 ttaaacgtgc caacggttac agcgaactgg agatcagcca gaagcgcagc atgctggaaa
     7141 acgtgatgct gaccgattcc gtggaaaccc ataaagcacg cctgcataaa gctggttttg
     7201 aacatagtga gctgtggttc cagtgcttta actttggttc actggtggca ttaaaagcag
     7261 aggacgctgc atgatcgact ttggtaactt ttattctctg attgccaaaa atcatctttc
     7321 acactggctg gaaacgctgc ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg
     7381 gctgtttaag cagtggtcca acgcggtgga atttctgcct gaaattaaac cgtatcgtct
     7441 ggatttattg catagcgtaa ccgccgaaag cgaagagcca ctgagcgccg ggcaaattaa
     7501 acgcattgaa acgctgatgc gcaacctgat gccgtggcgc aaagggccgt tctcactgta
     7561 tggcgtcaac atcgataccg aatggcgttc cgactggaaa tgggatcgcg ttctgcccca
     7621 tctttctgat ttaaccgggc gcaccattct tgatgtcggc tgtggcagcg gttatcacat
     7681 gtggcgcatg attggcgcag gcgcgcacct cgcggtggga atcgacccta cgcaactgtt
     7741 tctgtgccag tttgaagcgg tgcgtaaact actgggtaac gatcagcgcg cacatttgtt
     7801 accgttaggt attgaacagc ttccggcatt gaaagccttt gataccgtct tttcgatggg
     7861 cgtgctttat catcgtcgtt caccgctgga gcatctctgg cagttaaaag accaactggt
     7921 gaatgaaggt gaactggtgc tggaaacgct ggttattgat ggtgatgaga acacggtgct
     7981 ggtgccgggc gatcgttacg cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc
     8041 gctgaaaaac tggctgaaga agtgtggttt tgtagatatc cgcattgcgg atgtgagcgt
     8101 taccaccaca gaagagcagc gacgcaccga atggatggtc accgagtctc tggccgattt
     8161 tctcgacccg catgatccgg gtaaaacggt ggaaggttat cctgcgccta aacgcgcggt
     8221 gctgattgcg cgcaagccgt aaaggtctgg tattactgcc ggatgcggcg tgaacgcctt
     8281 atccggccta caaaaccttg ctaattcaat atattgcagg gactatgtag gccttataag
     8341 catagcgcat caggcagttt tacgtttgca taacctcagc gcccgtttcc gggcgctttt
     8401 cacgtcttat gcctgaacag ctggctgatc aaacgccgtt aacttcggtg cattgcccgt
     8461 atatttttcg atatacacca gcgcagagtt accggcacag gcatttgcca gctgagagct
     8521 ggggatatcc gccgttaaca cgttcgcact gccgttttta cacaagccat tttccagatc
     8581 tggccatgca ccttcatgca cgcataccac gccttttttg atcccgtcag tcaccaccgc
     8641 gcctgtcaga atctgaccac gtttgttcca gacacgcacc agatcgccat tcgcaatacc
     8701 aaagcgagca gcatcttcgg tgtgaatagt gatcggttca cgatcggcaa ccgcatattt
     8761 tttacgcagt tccgcatagt taagctgact gtgtaaacgg tgtgccggat gtgcggtcag
     8821 aagctgcaac tgcttctcgt cggcggaacc tttccactca tcaggcgcaa gccaggttgg
     8881 gtgtgccggg caatccttat agccaaattt ttccagcgtt ttggagtaaa tctcaatttt
     8941 gccgcttggc gtacccagcg catttttcac cggatcggcg cggaaatcac cataacgaac
     9001 gtattgttcg ttcttctcac tgcggcgcat ttcgatcagt ttattttgct gccagaaagc
     9061 attaaacatc ggcatagtca cgcgttgcgc acgggcacct ttctgagcgg catcatagaa
     9121 aaatttcagc cacgccattt catctttacc ttcggtatag atctcttttc cgccaggttt
     9181 gagtaattca gcaagatcgg caaacacgtc aaaatcgttg cgcgcttcaa attgcggagc
     9241 gacagcctgc ttcatcggca caatatgctg gttgctgtaa tcaccggtca tcgtcaggtc
     9301 attgcgctca aacgatgtgg tgatcggtaa tacgatatct gcatgtttag cagctgcggt
     9361 ccagtagcat tcagaaacga cgatcatctc cggtttctgc catgctttaa tcagacgatt
     9421 agtatcctgg tgatgggtaa agttaccgcc acccgcccac cagatcattt tgatattcgg
     9481 atacgtttgt tctttaccgt tatgctgata tttgccgcca ggattttcca gcgcatcgac
     9541 gatacgggca acaggaatcg ccgtcattcc gccatcatca gcggcctcgc tggcctgtcc
     9601 ggcaatcgcc gcagacattt ccggcagcac gccgccaaca cgcgtcgggt tgccaccgtt
     9661 ggagtaatga taagagaatc caaaaccgcc gcccggtgta ccaatttgcc ctaacatcgc
     9721 cgccagcgtc accagcatcc agtgtttctg ttcaccgtat tgctggcgtt ggatccccca
     9781 gcctgccatc agcatcgtgc gattcgccgc catcagttct gcaagtttga caatctgcgc
     9841 ttccggtacg ccagtaattt ccgccgccca ggccgcgctc ttcggcgtat tatcgctctt
     9901 acctgtcaga tactcttcaa actgcggata cccggtagtg tatttttcaa ggaacacttt
     9961 atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct aacatcagtg ccacgtcggt
    10021 gcccatattc ggtgcgatcc aggtggcatt atcgccaaag aattcgatgg tttcggagca
    10081 gataggatca atggcaatca ctggtttgcc agattttttc agctgatgga agtattccag
    10141 cccttgctca tcggtactgc tccaggcaat tttgagcgtg ttcagcgggt tcatccccca
    10201 cagcaccaca acctggctgt tttccagaat cagcggccag gaggtctgct gttcatacac
    10261 ctctacagaa ccgaccacat gcggcatgat cacctgtgcc gctccggttg aataatcgcc
    10321 gctatgcccg gaataaccgc ccgccaggtt catataacgt tgcagtaagg tttgcgcttt
    10381 atgcaacacg ccagaagaac gccagccgta agatccggca aaaatggccg atggtccgtt
    10441 agctttacga atacgatcgt gttgctcatg aatcagtttt aatgcttgtt cccagctcac
    10501 ctgtacatag gtatcttcgc cacgaccttt cgccggttgc agtggattat cgagatagct
    10561 ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt acctgatccg ccgccgtaga
    10621 ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc gaagaaacaa tcttgccgtc
    10681 cttcacttct acgttcatcg ctccccaacg gcccgcggtg agaattttgc cgcccttctc
    10741 ttctgcccat gcaggtaacg gtgcagccga tgtcaccacc agcgcccctg ccgcaatacc
    10801 gctatgttta ataaattcac gtcttgttaa tgtcataact tcctccctga tcaacgagga
    10861 tcactgtttc tcggtaatat ctttggcgtt gtactggaag taccgcgtca aaatgtccag
    10921 ttcgttttcg ctcatgctgg ttcgcgcacc cattcctttg gcaatggacg gccatgcatt
    10981 gacggtgtaa tggtcggagg caataggggc atggcaacca gcgcaatagg tatcgtcaag
    11041 tttttcagcg tattgccata gaggtttacg gtccgctaat gcgggatcgg taagcacacc
    11101 ctgtaaagac gcctgacgcc attgattgcc gtattcgtca gcctgccatt ccccgtttac
    11161 cgtcagcgcc ttgatacctt cttcacttaa tgtggctagc gccagccgtt gacctgccgc
    11221 caggtagagc gtgttttcac tgccctgcat ttgataaccc tgcaacagaa cgatcggctg
    11281 tttgccactg gcatcaacga cggtgagatc ggtaccagga ttcacggtag ccagttcgcc
    11341 tatgcgagaa gttttgaaag gataaatatg tgcgccatta gtaactgaag tagcggcctg
    11401 actttccagc tcatgcgccg cgttgtcatc catttttatt tctggcggaa aatgggcaat
    11461 gcctttatgg caatcgatac aggtttcgcc gtccttttgc gctttattat gcattttctg
    11521 cgcagattca ctttgcgagg caatatccat ggcatcaaaa gaatggcaac tacggcaggt
    11581 tgcagagtca gtggctttta attctttcca tactgtttcg gccatttcct ggcgatgagc
    11641 ttcgaactta tcatcactgt ctattttgcc gctaacaaat tcatgataaa tatctttaga
    11701 tgcttttaat ttagcaaata aataatccat ccctgacttt ggaatatggc aatcggcaca
    11761 ttccgcacgt atccctttct ggtttgaaaa gtggacagtt ccctgatatt cctcaaaagg
    11821 tttactcatc gagtggcagg aaaggcaaaa tgcggtatcc gacgttttat gtaaggcttt
    11881 ttgcgccagt aataacccgc cgccaccaac cacaaccgct atcagcaaaa acaataaccc
    11941 aatgcgtttt ttccctcgca tagattcctc taattaaatt ccaggacacc gataccattg
    12001 tataaataaa tacacaacga attgactact tgcttaattc ggcacaacac tgcctgaaat
    12061 aatcgataaa gaatatgatt tattacaatg taatcattaa ttgctaa
//
LOCUS       DAZKJR010000147        11979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKJR010000147 DAZKJR010000000
VERSION     DAZKJR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.148"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            8..340
                     /gene="hdeA"
                     /locus_tag="ACHOWS_004227"
     CDS             8..340
                     /gene="hdeA"
                     /locus_tag="ACHOWS_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFQ0049720.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            456..782
                     /gene="hdeB"
                     /locus_tag="ACHOWS_004228"
     CDS             456..782
                     /gene="hdeB"
                     /locus_tag="ACHOWS_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFQ0049721.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            846..1493
                     /gene="yhiD"
                     /locus_tag="ACHOWS_004229"
     CDS             846..1493
                     /gene="yhiD"
                     /locus_tag="ACHOWS_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0049722.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1545..2315)
                     /locus_tag="ACHOWS_004230"
     CDS             complement(1545..2315)
                     /locus_tag="ACHOWS_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFQ0049723.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(2312..3304)
                     /gene="shuU"
                     /locus_tag="ACHOWS_004231"
                     /gene_synonym="chuU"
     CDS             complement(2312..3304)
                     /gene="shuU"
                     /locus_tag="ACHOWS_004231"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFQ0049724.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(3353..3976)
                     /gene="chuY"
                     /locus_tag="ACHOWS_004232"
     CDS             complement(3353..3976)
                     /gene="chuY"
                     /locus_tag="ACHOWS_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFQ0049725.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFINRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(3976..4470)
                     /gene="hutX"
                     /locus_tag="ACHOWS_004233"
     CDS             complement(3976..4470)
                     /gene="hutX"
                     /locus_tag="ACHOWS_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFQ0049726.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(4483..5820)
                     /gene="hutW"
                     /locus_tag="ACHOWS_004234"
     CDS             complement(4483..5820)
                     /gene="hutW"
                     /locus_tag="ACHOWS_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFQ0049727.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLTQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(5840..6754)
                     /gene="shuT"
                     /locus_tag="ACHOWS_004235"
     CDS             complement(5840..6754)
                     /gene="shuT"
                     /locus_tag="ACHOWS_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFQ0049728.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTATERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            7438..9420
                     /gene="chuA"
                     /locus_tag="ACHOWS_004236"
                     /gene_synonym="shuA"
     CDS             7438..9420
                     /gene="chuA"
                     /locus_tag="ACHOWS_004236"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFQ0049729.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            9469..10497
                     /gene="chuS"
                     /locus_tag="ACHOWS_004237"
     CDS             9469..10497
                     /gene="chuS"
                     /locus_tag="ACHOWS_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFQ0049730.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     MPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(10558..11088)
                     /gene="dctR"
                     /locus_tag="ACHOWS_004238"
     CDS             complement(10558..11088)
                     /gene="dctR"
                     /locus_tag="ACHOWS_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFQ0049731.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11241..11807)
                     /gene="slp"
                     /locus_tag="ACHOWS_004239"
     CDS             complement(11241..11807)
                     /gene="slp"
                     /locus_tag="ACHOWS_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFQ0049732.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3104 a         2813 c         2978 g         3084 t
ORIGIN      
        1 tattacgatg aaaaaagtat taggcgttat tcttggtggt ctgcttcttc tgccagtagt
       61 gagcaatgca gcggatgcac aaaaagcagc tgataacaaa aaaccggtca actcctggac
      121 ctgtgaagat ttcctggctg tggacgaatc cttccagcca actgcagttg gttttgctga
      181 agcactgaac aacaaagata aaccagaaga tgcggtttta gatgttcagg gtattgcaac
      241 cgtaacccca gctatcgttc aggcttgtac tcaggataaa aaagccagct ttaaagataa
      301 agttaaaggc gaatgggaca aaattaagaa agatatgtaa ttccgggaat gcgttacatc
      361 gtatttcctt gcatattgaa caggccggaa tatcttcttt aaaagcagct attcctcctg
      421 tttgtatata atctctatat tgaatgggtt acaaaatgaa catttcatct ctccgtaaag
      481 cgtttatttt tatgggcgct gtagcggctt tgtcactggt gaacgcacaa tctgcgctgg
      541 cagccaatga atccgctaaa gatatgacct gccaggaatt tattgatctg aatccaaaag
      601 caatgacacc agttgcgtgg tggatgctgc atgaagaaac agtatataaa ggtggcgata
      661 ccgttacttt aaatgaaacc gatctcactc aaattcctaa agtgatcgaa tactgcaaga
      721 aaaacccgca gaaaaatttg tataccttca aaaatcaagc atctaatgac ttgccgaatt
      781 aatgaggtac aagtaaaaag gagtagcaag ttgagccatc ttgctgctcc tttttgcatt
      841 tttatatgac agcagaattt attatacgac ttatacttgc ggtaattgcc tgtggcgcta
      901 ttggcatgga aaggcaaatg cgcggcaaag gagcagggtt acgcacacat gtattaattg
      961 gcatgggaag cgccctgttt atgattgttt cgaaatatgg ttttgctgac gtgctgtctt
     1021 taggtcacgt cggactggac cccagccgta ttgctgctca ggttgtgacg ggcgtcgggt
     1081 ttatcggtgc aggtaacatt ctcgttcgta accaaaatat tgtaggtctg acgacggcag
     1141 cggatatctg ggtgaccgcc gccattggta tggttattgg cagcggtatg tacgaactgg
     1201 gtatttatgg ttcagtgatg accttgctgg tgctggaagt cttccatcaa ttaaccttcc
     1261 gcctgatgaa caaaaattac catttacagc tgacattagt gaacggtaat acggtatcaa
     1321 tgctcgactg gtttaagcag caaaaaataa aaacggattt agtttcgttg caggaaaatg
     1381 aagatcatga agtggtcgcc attgatatcc agcttcatgc cacgacgtcg atagaagatc
     1441 tgttgcgatt attaaaaggt atggcagggg tgaaaggcgt ttcaattagc taatctgtat
     1501 agttatttat tcgcaggagt accagtgcgg tactcctgtt ggttttaatg atcgaggacg
     1561 atcaggggtg aatggttggc aggatcttta agtacggtaa tatccgcgcc gtaaagcatg
     1621 gtcagcgcct gctgtgtcaa tacgtcctgt ggtttaccat ttgcgataac ctttcctttt
     1681 tgcattaata caatacggtc tgcataacgc gcggcaagat ttagatcgtg caatacgcag
     1741 caaacattga actgccgttc gtgaactaac tggcgtaata aacggaacaa gtgctgctga
     1801 tggtgaatat ccagcgccga agttggttca tcaagaaata accatttcgg tgagggagtt
     1861 ggttcccaga gttgcaccag caggcgcgct aattgtacgc gctgttgttc accaccggat
     1921 aattgccgat agtcgcggtt tgctaacgcc tgacagtcac ataacgccat tatttgtgct
     1981 gtttcattat cctgattacc agtgcgatgc ggatgccgcc ccatctggat aacttcttgt
     2041 acgctgaatg gaaacgccat gtggctattt tgacgcatca cggcccggtg tttcgccagt
     2101 tcagtaatcg accattcatt taatggctta ttaaacaacc gacattcgcc gctatcaggt
     2161 tgaagatagc cggttaactg gcgaagcagc gtagattttc ccgcaccatt agggccgaga
     2221 atggcaacaa tttccccacc gggaaaagtc agcgagacat tatcagtcag acgccgcccc
     2281 tgaaggctat aaaccagatt ctgggcgctg atcatgtgat cctccggttg cgcagaatca
     2341 accacataaa ataaggacca ccaagcaggc tggttaataa tcccacgggc atttctgctg
     2401 gctgtaccag cgtgcgggca agcgtgtctg ccatcagcaa taaacaggct ccggcgaggg
     2461 cggagcaagg gattagccag cggtgatttg ctccggtagt catgcgaatc agatgcggga
     2521 tcaccaggcc aataaagccg ataatgccgc ttaccgatac ggcggcacca acgagcagtg
     2581 agctcactaa cagtaattgc tggcgtttac gcttaacgtt cacgccgagg taatgggctt
     2641 cttcatcacc gagctgcagt aaattcagcg ttccggcgag acaagttgtt gcgataatgg
     2701 ccggtaggat gaatgaactg gcaaccaata acgtcggcca ttgcgcctgt ccaagattgc
     2761 ccatcatcca caatgtgagt tgtcgtagct gctgctcatc gccgatatag ctcaggatgc
     2821 cgatcgctgc gccgcacagg gcattgatgg cgataccggc gagtaataat tgcatcatgc
     2881 tgccatcgcg atggcgctgc gtgatgagaa aaatgatggt gcagaccact aaacttccgg
     2941 caatggcaaa caccatttgc tcatagagca ccagcaccac tgggaaggag aagggcagca
     3001 caatagcaac gccaaccatt aatgcagaac cgctactgac gccaagtaat ccagggtcag
     3061 ccattgggtt gcgaaaaagc ccctgcataa ttaccccggc ggttgccaac gctgcaccta
     3121 ccagcaccgc cagcaaaacc cgtggtagac gaatattgag ccagatatcg cggtattcct
     3181 catcaaaaag ggcatcaaaa ctgatcttta acgctccctg actggcacca aacagcacca
     3241 gagctagcaa taatagtgat agccccataa agaccctggc ggaagagagt gaatccttga
     3301 gcacgcaaaa ctccgttacg acattacggc gcggacatgc cgcgccgggg gatcacgccg
     3361 gtttcaggtc cggttcaatc agactgtaga cctgctggtt aagtgctggc gcatttgcca
     3421 gttcatggat atgtgcggcg acatcagcac ggttaataaa accatgacac tcctgatttt
     3481 gtatccgttg tgctttgccc gttgccgcac catcaagcag accgccaggg cggagaatgg
     3541 cataatcaag ctggctggtt tgcaaccagc tttccgccag cgttttttcc cgtaccgcct
     3601 gaccaaacgc agcctttgcc cgctctgata aaaacggcca gctatcgccg catcccagtg
     3661 acgtcaccag aatcatgcgt gtgatgcctg ctttctcggc ctcgtcaatc actgttctgt
     3721 gcgccagata atcctgagct ccgcccattg ttgagatgat aagcgcatcc gggccagctg
     3781 cgcggcaagc agccgcgact acgctggcat cacaggcgtc gcctgtaaaa acctgtacgc
     3841 cttgttgcgc cagtttcgtg gcggcatcgg catgacgaat gacagcaacg accggacgtt
     3901 gttccgccag cgccagttca agcgttctgg cacccacacc ttttccgcct gcgccaaaga
     3961 gtagccacgg tgtcatcagg cgtgttcctt taacgatgct gccagagcat ggaaggcact
     4021 gacttgttcg ctcaggagct gacgatgatc atccctaccg aggaagattt tcagcattgc
     4081 gctgccttcc ttattgaaaa agagaataga ggcggtatcc atgcccataa atttacgttc
     4141 gatcaaggca atatgcgtgc agttttctgc tttgatatgc ccggacatgc cgtgctttcc
     4201 gcgtaggttg aaatagccgt ggcgatggaa acccgaaggc agttcgccgc taaattcgag
     4261 tattacatcg gtagtatgta ctaacgtggt gacgttgccc cattcgcaga cggtatccca
     4321 gacagtatcg aatttatcac ctgacacaac agtcggagag ggcagatttt tcactacctc
     4381 cagcagcgtg gtgttgtatt gttcggctat cgcctccagc gtaccgtctg gctccgtttt
     4441 caaaaattcc tgtaacgaga catggctcat aaatcactcc tgttatggtt tttcaagcgc
     4501 aattgctggc gcattaagta cctgaatcag ttcattaaga gactgcaaaa tgttgcttgc
     4561 ccagaaacga ccttcattgg tcaaccgcag acacgttgac gcatcgcggc ttaagccttt
     4621 ttggtgccac tgggccagta acggcgcgag tttttcggca tgtggcgtaa gttcgtccag
     4681 cggtacacgc gccgtttcaa tgcccgactg caaggtatga cgccactgcg cgttacgttc
     4741 ggcgttacgc atgatcatca tcagcggttt tttacctgct gcgacggatt catgccaggt
     4801 ctgaagattg cgttcgttca tccaggagta accattaatc gaaccgccgg ctccggaacc
     4861 aaaggccaga caatcggcac cttgtttaat cagcaggtta tagagattgc gttcgcgtgt
     4921 ggtacggccc cagtggctgt tactgatgca gcgccaaccg gcatcgtcca taaaatcaca
     4981 cccttgcagg taaagatcgc gacgttctgc cggagagggc acggtagtac gcccattttc
     5041 cacggctttg cccagcggtg tattgggcag gacattgagc gcatagagat cgacgccgtc
     5101 gagaccgata tcgcgggcaa tagccagatc ttctccccag gtttgcgcat cctgacccgg
     5161 cagaccaaac agcaggtcac agaccaccgc ggcacggtcg cgtttgacca ggctttccat
     5221 aaacgcaata gcggttgggc catccgaggt gcgggccatt ttcttgcgga ttttgctgtt
     5281 aaacgactga ataccaattg agaagcggtt tgctccagca tcaaggcaag catcgattcg
     5341 ctcagcgtca aaattcagta cccggccttc aatagtgatt tcacaatcag gggccagtgg
     5401 cagcttttct cgtaatgtgg tgatgattct ggccaaatcg tgtgctgaaa gcgcggaagg
     5461 cgtaccgcca ccgaaataga ccgcgtggat gggggcagac tgatgcaata cgctgtctgc
     5521 ttccatttct atttcacgaa tcagggcgtc ggtataatga gcacacgcat cgtcattaaa
     5581 acggttctga taaaaaccac aaaacgtgca gtgtgtcgca caaaaaggaa tgtgtagata
     5641 aaccagtcgc ttacgtggtg ataccgtttg attgagcatc tcttgccagg tctgtgtcag
     5701 ttgctctttg gcaactggaa tggccccacg aaacggcatc atggcgcgcc tgtctttaaa
     5761 tggctgatca ccgtctaaag caaaatgtgg cgtgagatcc agggtgttat ttgtgttcat
     5821 tcgtctgatt taaatccatt tattgcggcc aaagctgctg gtgtaaagac tcaacgacat
     5881 cagcaatacg cggacccatt cccagaatca ggttttgatc aacggtgata atgcgctggt
     5941 ttttccatgc ggcagtgtgg gtaattccgg caatagaacg tagccgatta atatcgccat
     6001 cgaccatttg tgaggtgacg acaatcactt cagggttagc cgcaatcagc gattccgcgc
     6061 tgtagctttt gtactgctgg tgggtagcga cgttttctgc tccggcaagc gaaagaatgg
     6121 cgtccgcgac gctgccttta ccggcaacct gtggcgcact tccgcctgcg gagagaataa
     6181 acatggcttt aaccggggct tttttggccg ccacgttttg ctgtactcgc tccaggcgtt
     6241 ggttgatctg tgtcaccagc gcctcgcctt gttcaggaac ctgtaacgtt tttgccagtt
     6301 ggcgaatgtt ggcgtacatc tgctcaaggg tggctggtac acgcggcaga gtgacgacgt
     6361 tgactttttg cgcccgcagt tggtcgagca caatttgcgg tcctgcatcc tgccaggtaa
     6421 ttacgctatc cgggcgaagt gacaaaatgc cttcgctgct gagttgtttc cagtaaccaa
     6481 tatgcggcag tttggcggtt tctggtggat aagatgtcgt ttcatcgaca ccaaccacgc
     6541 gttcgccagc gcccatcgcg tagattagct ccgtcagcga tcctcctgcg accacgatac
     6601 gttcggtagc cgttacgcta aaagcccagc taagagccag tactgccgta aacaattttt
     6661 ttcgttttac cgcggtcttc gcggcagagg ccacggctga aatacataac gtcagtgggg
     6721 aaaaaagaaa cggcctggtg atgatccttg gcataatatg agaactctta tcaaattgag
     6781 aatgatcatg attatcattt gagttatata tgaaatacaa ccgtcgattc aatacgcacg
     6841 gcgttacaag cgtatttatt tttgattttt atcaagatgc ttcgtcagct aacgttcttg
     6901 ttaccgacta aatttcagcg cgggaaaaag ctgagaaccc agagatatcg cggcttgcag
     6961 agcaaagaga agagaaaatt atcactgtta acgacaaagt ttagtgatga ggtcatgggg
     7021 gaataaccat aaaaacaaag tggtttccag ggcgcaggat aagtttcata accccctgtt
     7081 tattgtgtta atggcggtta aaaaaaagct tgatcataaa aaattgatcg ataataattc
     7141 tcattatcac gtttgcattt aacaaaatac ggccatatgg ataacaaagc catatcattt
     7201 acaaggggta acgcacagcg ctcttcactc ctgatggggc atatcggttt gcctgacaaa
     7261 gagggcgtgc ttttgcgtat ttgccgagat accaggcaat ttcatgggat ataaacgcct
     7321 gtaaactcac ccggcgaata ttatcgctct gaattttaaa taaatttatt tttcgccagc
     7381 caccccggta ttaacccagg cagccagcat tttttatttt cctcatggag aatcgctatg
     7441 tcacgtccgc aatttacctc gttgcgtttg agtttgttgg ctttggctgt ttctgccacc
     7501 ttgccaacgt ttgcttttgc tactgaaacc atgaccgtta cggcaacggg gaatgcccgt
     7561 agttccttcg aagcgcctat gatggtcagc gtcatcgaca cttccgctcc tgaaaatcaa
     7621 acggctactt cagccaccga tctgctgcgt catgttcctg gaattactct tgatggtacc
     7681 ggacgaacca acggtcagga tgtaaatatg cgtggctatg atcatcgcgg cgtgctggtt
     7741 cttgtcgatg gtgttcgcca gggaacggat accggacacc tgaatggcac ttttctcgat
     7801 ccggcgctga tcaagcgtgt tgagattgtt cgcggacctt cagcattact gtatggcagt
     7861 ggcgcgctgg gtggagtgat ctcctacgat acggtcgatg caaaagattt attgcaggaa
     7921 ggacaaagca gtggttttcg tgtctttggt actggcggca cgggggacca tagcctggga
     7981 ttaggcgcga gcgcgtttgg gcgaactgaa aatctggatg gtattgtggc ctggtccagt
     8041 cgcgatcggg gtgatttacg ccagagcaat ggtgaaaccg cgccgaatga cgaatccatt
     8101 aataacatgc tggcgaaagg gacctggcaa attgattcag ctcagtctct gagcggttta
     8161 gtgcgttact acaacaacga cgcgcgtgaa ccaaaaaatc cgcagaccgt tgaagcttct
     8221 gatagcagca acccgatggt cgatcgttca acaattcaac gcgatgcgca gctttcttat
     8281 aaactcgccc cgcagggtaa cgactggtta aatgcagatg caaaaattta ctggtcggaa
     8341 gtccgtatta atgcgcaaaa cacggggagt tccggcgagt atcgtgaaca gataacaaaa
     8401 ggagcaaggc tggagaaccg ttccactcta tttgccgaca gtttcgcttc tcacttactg
     8461 acatatggcg gtgagtatta tcgtcaggaa caacatccgg gtggcgcgac gacgggcttc
     8521 ccgcaagcaa aaatcgattt tagctctggc tggctacaag atgagatcac cttacgcgat
     8581 ctgccgatta ccctgcttgg cggaacccgc tatgacagtt atcgcggtag cagcgacggc
     8641 tacaaagatg ttgatgccga caaatggtca tctcgcgcgg ggatgactat caacccgact
     8701 aactggctga tgttatttgg ctcatatgct caggcattcc gcgccccgac gatgggcgaa
     8761 atgtataacg attctaagca cttctcgatt ggtcgcttct ataccaacta ttgggtgcca
     8821 aacccgaact tacgtccgga aactaacgaa actcaggagt acggttttgg gctgcgtttt
     8881 gatgacctga tgttgtccaa tgatgctctg gaatttaaag ccagctactt tgataccaaa
     8941 gcgaaggatt acatctccac gaccgtcgat ttcgcggcgg cgacaactat gtcgtataac
     9001 gtcccgaacg ccaaaatctg gggttgggat gtgatgacga aatataccac tgatctgttt
     9061 agccttgatg tggcctataa ccgtacccgc ggcaaagaca ccgataccgg cgaatatatc
     9121 tccagtatta acccggatac cgttaccagc accctgaata ttccgatcgc tcacagtggc
     9181 ttctctgttg ggtgggttgg tacgtttgcc gatcgctcaa cacatatcag cagcagttac
     9241 agcaaacaac caggctatgg cgtgaatgat ttctacgtca gttatcaagg acaacaggcg
     9301 ctcaaaggta tgaccactac tttggtgttg ggtaacgctt tcgacaaaga gtactggtcg
     9361 ccacaaggca tcccacagga tggtcgtaat ggaaaaattt tcgtgagtta tcaatggtaa
     9421 tcatctgccc cgatatttcg gggcatttat ctggaaggaa gagagacaat gaaccactac
     9481 acacgctggc ttgagttaaa agaacaaaat cccggaaagt acgcgcgtga catcgcaggt
     9541 ttaatgaata ttagcgaagc agaactggca tttgcgcgcg tcacgcacga tgcgtggcgg
     9601 atgcgcggcg atatccgtga aattctggcg gcgctcgaaa gtgttggcga gaccaaatgt
     9661 atttgtcgta atgaatatgc agtccatgag caagttggtg cgttcacaaa ccagcatttg
     9721 aacggacatg ccggattgat cctcaatcca cgcgcgctgg atttgcgtct gtttctcaat
     9781 caatgggcca gtgtttttca cattaaagaa aatacgactc gtggcgaacg ccagagtatt
     9841 cagttctttg atcatcaggg cgatgcatta ctaaaagttt atgccaccga caataccgat
     9901 atggcggcat ggagtgagct tctggcacgg tttatcgccg atgagaatat gccgcttgag
     9961 ttaaaagccg ttgatgcgcc agttgttcaa acgcgagccg atgccagtgt ggtcgagcaa
    10021 gagtggcggg cgatgaccga cgttcatcag ttttttacgt tgctcaagcg ccacaacctg
    10081 acgcgccaac aggcgttcaa tctggtggca gatgatttgg cctgcaaagt atccaacagt
    10141 gcgttggcgc aaattcttga atctgcacag caggatggca atgaaatcat ggtgtttgtt
    10201 ggcaaccgtg gctgcgtaca gattttcact ggtgtggtag aaaaagtggt gccaatgaaa
    10261 ggttggctga atattttcaa cccgacgttt actcttcatc tattagaaga gagcattgct
    10321 gaagcctggg ttacccgtaa accgaccagc gatggctacg taaccagtct ggaattgttt
    10381 gcccatgatg gtacgcagat agcgcaactt tatggtcaac gtacagaagg cgaacaggag
    10441 caagcgcaat ggcgtaagca aattgcttcg ctgataccgg aagtcgttac tgcataacgt
    10501 ataattaacg ggaggtctgg ctatttattg ctgggcctct ttatattgat caatcgttca
    10561 caccagataa tcaatatgct gatggcgtac caactcattg atccgcttaa gattgaggat
    10621 catcatgata ttgtgtttat ggcagtagaa cgtttttagc gatattttca gtttctccag
    10681 aatatcttcc gttgtcattt gattcgcaat gcagctcaga acctgatgct gcttgcgcga
    10741 aagggacaca tccgcttcag gcacagagca gtaactgcca ttgatgagtg cgacaaagtc
    10801 gatgatggtg atagttcgcg gaacaaaggt caccggtact ttccccaggc agcgtttaat
    10861 attaaaggga gagaaaataa tcacatggac aggttttaat cgttcaatct gagtgagcaa
    10921 attagaatga aatacattat tcattaatgt atcaataatg acgaaggcat gctgatgcaa
    10981 cattacgtcg atctcttctt cgttctgtat atgaacgaca ttacctttac tcagaatgtt
    11041 tttcatcgca gtgaagaaca tcgtatccct ggtaattata agaaacataa ccgactcctg
    11101 gtgcggacat taactttata atcagtttgc caaaggtaat taacaggaca gggcttataa
    11161 taatagttag ctggctgtgt attaatttga tgtaatgcat gatgcagatg ctgagaaagc
    11221 atctgcatct ctcggtggtg ttatttgacc agctcaggtg ttacttgact caccgcattg
    11281 gtgtagtaag gcgcgcccca gcccggttcc ggccagaatg caccatagcc gtaatcccac
    11341 aggttatagg tggtattaac cacttctctc aaatgccaca cctggatgcc ctgcatattc
    11401 acttccagga agttatacgg gactttattg ataaagccag gttgttcacc ctgaatggta
    11461 ccgaggatgg taacaaagtg attacgatag ttcactggat caaggaagcc gctttgtctg
    11521 gcgagcagtc ggccctgata gttggcttca atatcaggct tcgcatagct atccaacggt
    11581 aatacagcga tttctaacaa cgtatccgtt ttgccattga taacgttgat aaccttcccg
    11641 ccaaagcgcg cttgttgacc aacatataac cccggctggt tatgaacagc aacaaaactt
    11701 ttttgaatat caggttggtt attgcctttg atattttgcg gaattgaact acatgcggca
    11761 agcaaaaatg aaaggctgag gatgagtgca ccttttgtca tgttcatgtt actatcctta
    11821 tcaacaaata ttaaaaccat aatagatatt tctttatagt tttcatctga ttctgaggcg
    11881 atatgcctga tagtaacgta aacctgtctg atgtttatat cccttctgtt tagtttaatt
    11941 ctgttaattt tggggcgtga catgagtatc catcgcccc
//
LOCUS       DAZKJR010000148        11936 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKJR010000148 DAZKJR010000000
VERSION     DAZKJR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.149"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(214..693)
                     /locus_tag="ACHOWS_004240"
     CDS             complement(214..693)
                     /locus_tag="ACHOWS_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049733.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMFLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            complement(805..2049)
                     /locus_tag="ACHOWS_004241"
     CDS             complement(805..2049)
                     /locus_tag="ACHOWS_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFQ0049734.1"
                     /translation="MTLPVDSLIAYTLEKMQERIVQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEGRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSDSEPSEKSEGYHRVRKPNHYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRSE"
     gene            complement(2067..2534)
                     /locus_tag="ACHOWS_004242"
     CDS             complement(2067..2534)
                     /locus_tag="ACHOWS_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049735.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            complement(2538..2957)
                     /locus_tag="ACHOWS_004243"
     CDS             complement(2538..2957)
                     /locus_tag="ACHOWS_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFQ0049736.1"
                     /translation="MAEINFQVLRENDAGELTGVATAGRPVARHFDDGLTLEVNRTCT
                     TGERNANSALYGAVWRAAKDMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAE
                     SSVALKSTRDLVGSGGVPCVLREIRRMSTTVIHIKGE"
     gene            complement(2972..3580)
                     /locus_tag="ACHOWS_004244"
     CDS             complement(2972..3580)
                     /locus_tag="ACHOWS_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFQ0049737.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            complement(3577..4302)
                     /locus_tag="ACHOWS_004245"
     CDS             complement(3577..4302)
                     /locus_tag="ACHOWS_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFQ0049738.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGDRY
                     RRCVTFYGFSERPQVAAYAFDVLCRHLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            complement(4295..6028)
                     /locus_tag="ACHOWS_004246"
     CDS             complement(4295..6028)
                     /locus_tag="ACHOWS_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFQ0049739.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGDPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHNSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVSIFPGTEREQHGITRPLKAVSDTDTERTEKCSRPSQEESAGRGDSGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDDAAGAVLASVMTGDTAPGTTDALPASVTH
                     FAVGRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            complement(6028..7395)
                     /gene="dnaB-PI"
                     /locus_tag="ACHOWS_004247"
     CDS             complement(6028..7395)
                     /gene="dnaB-PI"
                     /locus_tag="ACHOWS_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFQ0049740.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            complement(7743..8645)
                     /locus_tag="ACHOWS_004248"
     CDS             complement(7743..8645)
                     /locus_tag="ACHOWS_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049741.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVERQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGSHPDVSVYTERCAQ
                     GMSLSPVHSARQCPVQPTDSAETE"
     gene            complement(8881..9090)
                     /locus_tag="ACHOWS_004249"
     CDS             complement(8881..9090)
                     /locus_tag="ACHOWS_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049742.1"
                     /translation="MCVGCGYSGVRDGAGKNALVWVKVNPESGLGLCSGCDSFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
     gene            complement(9423..10310)
                     /locus_tag="ACHOWS_004250"
     CDS             complement(9423..10310)
                     /locus_tag="ACHOWS_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0049743.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLEMIVLSATDSVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTMHSLACELLPEW
                     RAFFDALLENGGKSLWAEN"
     gene            10626..11807
                     /locus_tag="ACHOWS_004251"
     CDS             10626..11807
                     /locus_tag="ACHOWS_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0049744.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     T"
BASE COUNT         2816 a         3555 c         2782 g         2783 t
ORIGIN      
        1 acctcttctg ccactggcag cactgttcgc tttttaacct cttctgccac tggcagcgct
       61 gctcattttt taatctctgc tgccactggc agcactgctc attttttaat ctctgctgcc
      121 actggcaaca ccgctcattt tttaatcacc gttaccactg gcagcgctgc tcatttttta
      181 acctctgttg ccactggcaa caccgctcat tttttaatca ctgttgccac tggcaacatg
      241 gttaatgatg actactacct taacgttcag agacaaaaac agcattaatg tcgttatccc
      301 catcacgagc aactgaatgt cggttatatc cagatgagac aatgaccaga aaatcacaaa
      361 aaatgaaaag cattctgcca gcagactcag gaggaacata aattgcaggc cccctccgac
      421 tgtactgcct gtacgcgctt tataaagact gtatgtgacc cacagccagt tacttaacac
      481 caggaaaaac caccctgtgg cacatacgat ataacacata ctgttatcgt actgttcgtg
      541 cattgttttc agcgcagtga gcagggtctc tgtgcctgtc accagccccc agataaccac
      601 cactccggca atcacgcagg cacaaatttt agcgatcaac aggccacttt ctttcatggc
      661 tgcactcaga ctttcctgtt tgtatttctg catgctattt accaccttgt ttcacctgac
      721 cagttcccga ttctgttttc tttttaacca gtcgctggcg gcactggttg atttttgttc
      781 ccgcgaacat agcccgtatt gcaatcactc agatctcatc gcagaacatc tgcgcagcga
      841 ggcccgtagt ccagccacaa tgccactcag acgttctgct gaaatcggtt cagtacgttt
      901 ccccgatgaa actgattgcg cagtagctcg ccccctttct cctgccagta cggaggctgg
      961 cacaacaaca cgttgtttct gcttcggcgg ataaaaattt ccctcccggg ctcgcttcag
     1021 cactgcaagc aaccagccca caggattctg cagcgagtgg ctgcccagca tccctgacag
     1081 actttccaga accccctgcg ccagttcttc cggcagcgcc tgcaactgac tggtcagcat
     1141 aatggcctcc tcctccctga tttgccctgt cagcgcttca ggcaatacgt acgtgttttt
     1201 aatcacactg tgtgtgatat tacgtacgta atggttcggt ttccgaactc tgtgataacc
     1261 ttctgatttt tctgatggtt cggaatccga actcagtatt ttttgcccct gtttttcact
     1321 gagttcggaa tccgaactca gcattttcaa taccgaactc tgtgtttcta cccccggttc
     1381 ggtttccgaa cccggctgca ttctgccctc agtcacccgc tgacgggccg ccatctgtcc
     1441 cggtgtctgg gcactcccta tccgggcttc cagttgactg atatggctgt gataatgacg
     1501 cattcccgga tcatttttta tttcgcacag aatatcccgg gccgtctgac ttatggtccg
     1561 gttccggctc atacaggcat ctgccaccat atccagataa cgtggatcca gtatttcagc
     1621 atcccggcac gtcaccggtt catcgtgctg ggcataaata ttgccccgta cccgcccttt
     1681 ctcatcacgc acccgtttgc acagactcag ccagcccgtg atccgcagca tcagcagcac
     1741 ccggctgaca gtctccctgg aggcttttcc tttccccggg gaagcaagct gcagttgcag
     1801 ttcgtcatat gtggggaaaa ctgcaccttc attattctgt gcatacagcc gaatcatcat
     1861 ccacgccatc ttatccagcg gagaaagtcg cgtgtccaga agcagtcgac gcggaatggc
     1921 atcatgcaca tttccggtaa acagcagtcc gctttgtatt tttccgtcct gttgtcggcc
     1981 tttctgcact atccgctctt gcattttttc cagcgtgtac gcaatcaggc tgtctacggg
     2041 taatgtcatt acacttttcc tttttgttat acgtcagaca tccgtctgct ggtaacgggt
     2101 atatcgccca tattcaccag acagttacgc cggagatatg ccagcgcttc atcaggaacg
     2161 gtacattctg gtctggcttt tttcaggtta ctgattaatc gtttcatcag cattactggt
     2221 tcatgtgcac ggatccaccg tccccctgtt tgatcttcca gacagcgaat taacatctca
     2281 gctggcaaaa gaaccccgtg gttctcacac tcctttttct tctctgccgc agttaatttc
     2341 tgttgcaatt caacaattgc tgccagcgca gatttaatcg tttcactcgt cacccgacat
     2401 ggataagcac gattcctgcc ttctttcaga agcactgcat aaccatcaga gcggtagtga
     2461 taacagtttg ctcccaacca gatttcgtaa gtatcgccgc gcgaggagat acccgttctc
     2521 tctgcaatag ccatatctca ttcccctttg atgtgaatga cagtagtgct cattctcctg
     2581 atttcccgga gcacacaagg aacaccacca cttccgacca gatcgcgtgt actcttcagg
     2641 gcgacacttg attccgccca gctttttcct gaaggaagtt ctttcacacg aacaaaacca
     2701 gctgcccgaa gagaggctcc ggattcatca gccctggtgt atgtaatgca acggcgatag
     2761 cccatatctt ttgccgccct ccagacagcg ccataaagtg cgctgttagc gttacgttca
     2821 ccagtagtac aggtacgatt gacctccagt gtcagcccat cgtcaaagtg gcgggcaaca
     2881 ggtctgccag ctgtcgccac tcctgtcaat tcacccgcat cattttcccg cagtacctga
     2941 aagttaattt cggccatgtc atttgtcctt gtcattctgc tttttcccgg ttcagtttcc
     3001 ggcgactggc cgatacatca ttaaaccccg cagttccctg atgccatccc ctcacggcat
     3061 gccataccgc cgtcagcggg atattcattt gctcagctgc cagcatcatg acatccagtc
     3121 cgtccgcact ctccacatcc gtgatgccgc tcttctgcca taaacgccac agctctgcac
     3181 tttcctcatc actcagtgtg atgccacgtc ccgggcgaac atcaataccg gcaatgcgac
     3241 ggcgcgccgc cacatccaca ctggaaagtc caaagtaaaa cgccatcagt tcaatggagc
     3301 ctcccagcgc cagtgcccgg tcaatgcgct gcagacgttt ctgctccgtc cgggcctgct
     3361 gcagcatacg cagcaggttt tcatgattaa tgtccagccg gataaccgac acttcagagt
     3421 ttgacaggta atgaatttcc tcaatggtca gtccctgcag catctgcatt tcctcccggg
     3481 ccagccccag agattcgcag cgacgcaggt agccgctctt gaggtccatc accagttgca
     3541 tcagcagacc gttggttgcc tgagacaagc tgttattcat gacgcctccc gtatcatcag
     3601 acccggcacc acctgtcggg cactctcccc cacaccatga tgcagtgagg catttttccc
     3661 ggcctcatac ccctgccagc gggccaggtc accgccgcga caggctttca tctcacggga
     3721 gtccaccgtc accatatcct gctgctccat ccagagacgc atcagccggc tttcctcttc
     3781 tgtcacactg aatggcatca ccacctgacg aatcccctga acccagccat cacgaaactg
     3841 agccgcccgg gcacgacggg tgctcagttt cagtctccgg ctctgtgtac gaagatacgc
     3901 atccgttgct tcccttagat gacggcacag aacatcaaac gcataagccg ccacctgagg
     3961 acgttcactg aaaccgtaaa acgtcacaca ccggcgatac cggtctccgg gcaccgcccg
     4021 ccagtcgtaa tacgcccggc agccaaatgt ctgtgtcacc accatgacca gacgaaacat
     4081 ccacgccgga actttttcag catcagaagg tgccccctgt gatgaagagg tctgcaccga
     4141 agaaagtccg gcatcctgct ccgtgatacc gtgtttcgcc atcagctgtt gcgcccttgc
     4201 aagcgccagc ccggcctcat gggcattgct gttattacgg ctcagctcca gcagtttcct
     4261 gacccgcctg atcagacgtt cctgatgcga catatcactc atcattctca tccccctcat
     4321 cgtcgtcggg taacacactg cgctgcaggg ctcgcagacg gcgcagcaca ctcagaagac
     4381 gcagcagctt cacggcatgg tcgtcatcca gcagtggcca cgcttccggt gcactgcccc
     4441 ctgtaagcag gtctttcaga ctcacgggga ggactgtccg cggtgcatca ccggccagcc
     4501 ccgccagaaa cgccactaca ggagaacatt tctccactgc tgccccgaaa ccggcagaca
     4561 ggtcgctttc cctgtccatg gcaatctctt cagcacaacc ggctgattca gccagttccc
     4621 aggccagacg gaacgcttca ttctgcagat gttcgatatc atcccgcccc acagcaaagt
     4681 gcgtcaccga cgcgggaaga gcatcagtgg ttcccggggc agtatctcct gtcataactg
     4741 atgccagtac tgctccggca gcatcatctt ccataacatc accactgaag acaggcttac
     4801 caccgtacat atccggctgt atctcaggac gcagggcatt ttcagcagaa gacgccccac
     4861 tgtcccccct gcctgcgctc tcttcctgag aaggtcgact gcatttttca gtgcgctctg
     4921 tgtcagtatc agaaacggct ttcagtggcc gggtaatacc atgttgctcg cgctcagtac
     4981 cggggaatat ggagactgtg ctttccggca tcgcctctgt atccgtatcc ggttgacact
     5041 ccggtacttc agaccggatc ccggataccg gtgccccggg ttgtaccgta ctgctgccac
     5101 ggactgaagg aggcaccacc accggttccg gctcaccaaa atgatgacga cggtttcgct
     5161 ctttcggatc cagttccagc aaccaccggt catagtccag ggagggatgt ggcagggcct
     5221 gcaacaaatc tccgataagc tcatcgcgga acatctccag tgaccacagt tccggcgaat
     5281 taaacttccg gcagctttcc ccgaacacgc ggtcaaattc cgcatccgta tcggctgcaa
     5341 cagcaaactg cccccataca cgttctgcgt cctgacgaag tgcgaggagt gctgtaacct
     5401 gtggacgccc cagtcctgac tccatcaggt cggggatcca ggggtacaga tacttcaggg
     5461 catcttccat tctgctgatc gtggagttat gtatcggcaa cccttcatgg gttaacaaat
     5521 cagccagttc acgaagagac actgttctgc ccagttgttc ctcatggatt tgtcttgcct
     5581 tgtggacccc ctgcgccttc tcgataaaag tcagttcacc gcgaacttca ttttcagcca
     5641 gatgaccaat cacacactgg agccgacccg gccagggttt aaacagcaca tgaatacggt
     5701 aaaaacgcgg atcccctgtt tctttccaga gttccgtcag tatctggtaa cgggtgtttc
     5761 ccccgtcact gaagatatac acatccggct cactgtccgg catacgggtg actttgggca
     5821 ccgtatccag cccgcgggca tgaatggaat ttttgatatc gtcataacgc ggattgcgcg
     5881 tggtgcgcgg gttatccgga ttcggacgta actggtccag cgtcagaacc atgggcgttt
     5941 cccccattgc caccacattg ccggcaccag tggtctgacg accgggctgc ataatagccg
     6001 ccccgagatc cagtggttta tgtgccatca cgcatatcct cccggcccgt cattctcaga
     6061 gggcgtaaac agtgtgtaac gaccgtcgaa cctgacccgt accgtacccg tgggcccctg
     6121 acgctgcttg ctgacaatga tttctgcctc cccctttgcc agtgagtcgg ggttatagac
     6181 ctcgtcgcgg taaataaaca taatcaggtc agcatcctgc tccagcgccc cggaatcacg
     6241 caggtcaccg ttattggggc gtttatcagc acgctgctcc acctgccggt taagctggga
     6301 gagggccagc accgggcagc ccagctcctt ccccagcgct ttcagggaac gggaaatttc
     6361 agcaatttcc tgtgtgcggt tttccagctc cggcgcacga atcagttgca gataatccac
     6421 cataatcagg gagggtttac cgtacagacg tgtataacga cgggcgcctg cacgcagtga
     6481 tgaaggcgtc tgctcacagc agtcatcaat tattatgcgc tcacccagag agcccattcc
     6541 tccgtcacca agcgccatac cgaccgcaga tgacaccctc agccagtcct catcatccat
     6601 gttgccactg cgcaaatgat tcagttccac acgccccagc atggaaagca tgcgcagaat
     6661 cagttgctcc ttcggcatct caatgctgaa taccagaacg ctctcttccg ttcgccgcag
     6721 tgcggcgctg agacacatgg atagcgccag agaggttttc cccattgacg gacgggcagc
     6781 cagcagaacc aggtcaccag gctgcaggcc acaggtcatt tcatccagac agtcaaatcc
     6841 cgtgggggta ccggtaacac caccggagcc gagcacactt tccagttgct gcagaacggt
     6901 atcaagcgcg gatgaaagag aggcttccgt gcgttcccgg agcgtcctgt tctgcgcaag
     6961 tgcaaacaga cgccgctctg cagcatccag cacatcccgt gatgtggcgt tcggtgccgc
     7021 cacatcagca atcagggtgt ttccggtggc ctgtaactga cgcagaacgc ttttctccac
     7081 cacaatgcct gcataggcca tgatgttggc cgcagaaggt gtgttcttgc tgagctcggc
     7141 cagataagca aagccgccca gtacgtccag cctgccctga tgttccaggt gctctgacag
     7201 tgtaatcagg tccagtggct ggccattcac cgccagttca gacatggcac ggaaaatagc
     7261 ccggtgctga gccacataaa aatcgtcagg actcagcatc agcaccactt catcccagcg
     7321 ttcattctcc agcatcagac cgcccagaac ggcctgctct gcttcgtatg cgcagagaac
     7381 agacatttca gccatggcgc cccctccccc tctgacagcc ggaaacctgg ctacgtgaaa
     7441 aaaggaaaaa gggtgccgga cgtacagctg tcgggaataa aatcccggta actgtctgta
     7501 cgttttcaga ccggtgagtt tgccgccccc ggtctgaagg tacggacagc attcgttcat
     7561 tcataacaag ttgttttaaa ggaaaaagtt tatgaataca gaaaaacaac tgccgtccgg
     7621 cattgaatct gaagtgaaat acaccctgca gttctggctg ctgagtaacg gaaaggtcat
     7681 gacggaaaaa gacgggctct gtaacccctt tcccctgaac tgtattgaat cggctcatcc
     7741 ttttactccg tttcagcaga atcagtgggt tgaactggac actgtcgggc cgaatggacc
     7801 ggtgaaagcg acattccttg tgcacagcgt tctgtataaa ctgaaacgtc agggtggctc
     7861 cctgaatgat gccggccggc ctgaatacgg ccggtgcatt gctcactggc acactgacgt
     7921 gtttgttgag aaaaaagcgc tgcctgactg gctgagagca ttccacactg acgtttcgca
     7981 ctcgtaaaaa cacgttcagc ccgacgggtc atattttcca gccacccgtc ggggaaaatg
     8041 tcagcaccat cacggggaaa caaaatcgct ttccattcag ggtatgagcg cggaatacct
     8101 ccggaagcat tccggctcag acagcgactc acagtgcagg cagcacggaa tgcatatgtt
     8161 gcgatgctct catcatatgt tgcgatgttc tcattattgc ctttctgccg ttccacccgc
     8221 tcttcagcat caaatgccag tgcagcaagg cttgcccagc acatgcagtt cactgaattc
     8281 cccggaacgc tctgcagctc ccggcgcacc atgttgacct cagcactgaa aaaagcgttc
     8341 tcatcgcctt caagggcagc cagatgcagc ggaagccaga cctgactgaa ccattcgcac
     8401 atccgttccc ggttactgac ggtcatcact gtctccgggc gggaaccgtc ctgaaaaaca
     8461 tgtccggtgc gtggagaact gtatgaaaca taaccaccgt gcaccgcaat gaccggacga
     8521 cctgacgccg gaagccccac acgacaccca ctggcaatcc gggtcatcag caggttcagt
     8581 tcagccagta attcagggcg ggttaagacc gccggaaccg gagaatattt cactgaggaa
     8641 gacatacttt cacctccata tctctcagcc cgccaggtgc gtcacttccc cgcagagaac
     8701 cggaaacctg gctatatgaa aaaagggaaa agggtgccgg acgaacagtt gtcgggaata
     8761 aaatcccggt aactgtctgt acgttttcag accggtaagt ttggcgaccc tggtcagaag
     8821 gtacggacag catctttgcg ctcataactg taccgaccgg gatatgcatt acaaagactg
     8881 tcataccgcc ccctccgtct ttcgctgttt gcaggaaaga ccacatgaaa aaagctggtc
     8941 atgccgctga cgacgctggc gttcctgcaa aagaaaggag tcacaaccgg aacacagccc
     9001 cagaccactc tcaggattca ccttcaccca gaccagagca ttcttaccgg caccatcccg
     9061 taccccggaa taaccacagc ccacgcacac cgtccacttc tgcagtacga tgagtgcagt
     9121 ttcgagtgat gcgcggagac gccgggaggc aataaggagc aacggctcat ggtcataaaa
     9181 aaccccgccg ttaccccctc cggattcaag cagcgggagt cctgcaggct cccggggacc
     9241 tgttgacatt tcccgtaatt cgttttcatt ccacatattg tggaaagtta tccggttcac
     9301 tgaacaacgc cattcctccg ccagacgctg atacaaatgc tttttcgctt ctgcgcggtc
     9361 agatgcatca acaaacacat gcacttcctc gtgtccgggt gctgaagcca cggcacgcca
     9421 tatcagtttt ctgcccacag gcttttccct ccgttttcca gcagagcatc aaaaaacgca
     9481 cgccactccg gcaacagttc acaggccaga ctgtgcatgg tgaccagcgc agaatcgctg
     9541 caacgggcag acactttttc cagacgatgc accggctgac cggctgcgtg cccctgttta
     9601 taaatattca ggtcgtaaat ctgtgttgaa agaagggtga ccgcaccttc aggcagcact
     9661 ttgctgtagc gaccggcgtt gataatgcct gtcagctctg ccagtgtctg tctggccagc
     9721 gccgtataat ccatgcagtt aaccagggtg cggactttcg gcaggggaat acccagttca
     9781 cggtagggca taaggttttc catgacattg accgtgccgc ggatgaactc acgcacatcc
     9841 ggcagaaccg gattcaccat ccccaccaca gagtccgtgg cggacagcac aatcatttcc
     9901 agcatgacag aacgtgcgcc ctgtgaatcc accacaatga cgtcataggt ctgaaaaagc
     9961 ggatgctgaa gcacattacg cagacgaagg cgaccatccg gcgcatgaag catggccgtt
    10021 ttcagttgct catgggggtc gttggagacg atgaggtcaa gccccctgac agccgtacgg
    10081 gaaatgatgt tgtcaggatg actgaggtca gccgtgcgca tcagtaattc aaaaagaccg
    10141 gccggcgctt catacgtcag cggaaagata ttgcttgccg tgggctgtgc atggtctccg
    10201 tctatcagca gagtacggag gccggcatct gccagaaaac cggcaagatt agcagactgg
    10261 gttgattttc cttcgccacc tttagtggat ataaccggta aaattttcat atagcacact
    10321 ctttaaagta tgcaaccaat cacttacact acggaaacca gccagaatat tcacttcact
    10381 acctgaatac tcgcaacacg gggattgaaa atccccgtgt ccttggttcg attccgagtc
    10441 cgggcaccac tttcacttcc agattcatct cccgaactcc acgaaactca agtataatca
    10501 catcgttaca agcaatctga acatcctgaa ctccaccgaa ctctactgaa ctctttctca
    10561 atcaagcttt cattgggggc accggtgggg gctcattatg ttcagtcagt ttcaggagcc
    10621 cccatatggc acttacagcc agacaggtcg aaacagccag acctaaagaa aaagactata
    10681 aactctctga cgaacgtggt ttatatctgc tggtaaaaac cacgggagcc cgctactggc
    10741 ggcttaaata ccggatagca ggaaaagaga aaaaactggc cctcggcgtc tatcccgacg
    10801 tctcccttgc tgaggccaga atcaaacgtg acgatgcccg aaaaatcatc tccgaaggtg
    10861 gtgacccggg cgaaaagaag cgaaaggaaa aactcactca gaaaatctct gccaccaata
    10921 cgttccatgc cctcgctacg gaatggcacc agcataaatc tttgtcatgg tctgaaagtt
    10981 acgccagaag cgtactggaa gcgctggata aagatatttt cccgtatctg ggcaaacgaa
    11041 gcgttacgga tatcctcccg ctggaaatgc tggaaattct gcgccgcata gaaaaacgtg
    11101 gctcgctgga aaaactgcgt aaggtacgtc aatactgtaa tcagattttt cgttatgcca
    11161 tcgccaccgg acgagccact gtcaatccgg catctgaact gaccagtacg ctggcggcgc
    11221 caaaagctgc acacttcccc cacctgagag cagatgagct ccctgttttt ctcaggaagc
    11281 tcgctgaata tcatggcagt cctgttaccc gcatggcgac aaatctgctg cttctgacag
    11341 gcctcagaac gattgagcta cggtccgctg aatggtcaga aattgatttt gataatgccc
    11401 tgtggacaat ccctgaaagc cgcatgaaaa tgcgacgtaa acatgtcgta ccactgtcac
    11461 gacaggtcac tgacattctg caacaactca aaactttctc cggacaatac cggctggttt
    11521 tcccgggacg ttgtgatatc aacaagccaa tgagcgaagc cagcatcaat atggtgctca
    11581 aacgtatcgg ttacgatggc agagcaaccg gtcatggttt tcgtcacacc atgagtacca
    11641 ttctgcacga acaggggttt aattctgcct ggattgaaat gcagttagct catgtggata
    11701 aaaattccat caggggtacc tataatcatg ccctgtatat tgatggtcgc cgtgaaatga
    11761 tgcagtggta tgctgactac attgattcgc tttctgagca aacctgatga atcagtgaca
    11821 taaaaataat gaataaccat ctggccccga aagccggatg gttttattta tgttttcttt
    11881 tttatttgct cacatcttct gaactgcccc tgcagacaat aatctgaaca ccagat
//
LOCUS       DAZKJR010000149        11913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKJR010000149 DAZKJR010000000
VERSION     DAZKJR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.150"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..1535
                     /gene="narU"
                     /locus_tag="ACHOWS_004252"
     CDS             147..1535
                     /gene="narU"
                     /locus_tag="ACHOWS_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFQ0049745.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1630..5370
                     /gene="narZ"
                     /locus_tag="ACHOWS_004253"
     CDS             1630..5370
                     /gene="narZ"
                     /locus_tag="ACHOWS_004253"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFQ0049746.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELLLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVF
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDMVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLAQHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5367..6911
                     /gene="narH"
                     /locus_tag="ACHOWS_004254"
     CDS             5367..6911
                     /gene="narH"
                     /locus_tag="ACHOWS_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFQ0049747.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLNVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6911..7606
                     /gene="narW"
                     /locus_tag="ACHOWS_004255"
     CDS             6911..7606
                     /gene="narW"
                     /locus_tag="ACHOWS_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFQ0049748.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7603..8283
                     /gene="narI"
                     /locus_tag="ACHOWS_004256"
     CDS             7603..8283
                     /gene="narI"
                     /locus_tag="ACHOWS_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFQ0049749.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8363..9256
                     /locus_tag="ACHOWS_004257"
     CDS             8363..9256
                     /locus_tag="ACHOWS_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFQ0049750.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFESPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPDKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGVAVILFHAEWAIEL"
     gene            complement(9352..10197)
                     /gene="nhoA"
                     /locus_tag="ACHOWS_004258"
     CDS             complement(9352..10197)
                     /gene="nhoA"
                     /locus_tag="ACHOWS_004258"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFQ0049751.1"
                     /translation="MTPILNHYFARINWSGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10372..10941
                     /locus_tag="ACHOWS_004259"
     CDS             10372..10941
                     /locus_tag="ACHOWS_004259"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0049752.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFYCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11095..11475)
                     /locus_tag="ACHOWS_004260"
     CDS             complement(11095..11475)
                     /locus_tag="ACHOWS_004260"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0049753.1"
                     /translation="MIIRPVKPSDVEPLLDMLLASGQFNQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCAPEVMTNDVWNLLMHWISPSHQRQGVGHLLINEIEKELTI
                     KQARLLLVETSSYVDFSAARAFYT"
BASE COUNT         2875 a         3043 c         3076 g         2919 t
ORIGIN      
        1 cattctgccc ccgcagttac gctttcttat ctattcttct aatcagcact tcacctgatt
       61 catccttaat tctaataact ggctattgcg ccttacctcc tgttttgtct ttttttcatt
      121 accaatgtgt gcatgtgagg aacaatatgg cactgcaaaa tgagaaaaat agtcgttatc
      181 ttttacgcga ctggaaacca gaaaatccgg ccttctggga aaataaagga aagcatattg
      241 cacgaagaaa cctctggata tcagtcagtt gcctacttct tgccttctgt gtctggatgc
      301 tatttagcgc agttactgtt aatctcaata aaatcggttt taatttcact accgatcaac
      361 tctttttatt aaccgcatta ccctccgttt ctggcgcatt attgcgtgtc ccctactcct
      421 ttatggtgcc tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta
      481 ttccttgcgt ctggctcgga attgccgtgc agaatccgaa tactcctttt gggatattta
      541 tcgttatcgc tttgctatgc ggttttgcag gtgcgaactt tgcttcgagc atgggcaata
      601 tcagtttctt ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag
      661 gaaacttagg tgtaagtgtg atgcagctgg ttgcaccgct ggtcattttt gtccccgtat
      721 ttgcctttct cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga
      781 atgccgcatg gatttgggtg ccattactgg cgattgccac gatcgccgcc tggtcaggga
      841 tgaatgatat cgccagttca cgcgcctcaa ttgccgacca gctccctgtc ttacaacgcc
      901 tgcatctctg gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt
      961 ctgcgggttt tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg
     1021 cgttctttgg cccatttatc ggtgccatcg cgcgatcggt tggtggtgct atttccgata
     1081 aattcggcgg cgtgcgggtg acgttgatca acttcatttt tatggcgatt ttcagcgccc
     1141 tgctgttcct taccttaccg ggcacaggct ccggtaattt catcgcattt tacgccgtat
     1201 ttatggggct gtttctgacc gcgggtctgg gaagtggttc tactttccag atgatcgccg
     1261 tcatctttcg ccagataaca atttatcggg tgaagatgaa aggcggtagt gatgagcaag
     1321 ctcaaagaga agccatcacc gaaacggcag ccgctctggg ctttatctca gccattggcg
     1381 cagtgggcgg cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc
     1441 cggtgggcgc gatgaaagtg tttttaatct tctacatcgt ttgtgtgcta ctgacctggc
     1501 tggtttatgg tcggcggaag ttcagccaaa aataagagat cgatgcctga cacgtcttat
     1561 caggctacaa ccgtgtcgca tccgacaagg catcagatcc caattcattc ctggagcagg
     1621 agttatgtca tgagtaaact tttggatcgc tttcgctact tcaaacaaaa gggcgaaacc
     1681 tttgccgatg gtcacggaca ggtgatgcat agcaaccgcg actgggagga cagctatcgc
     1741 cagcgttggc agttcgacaa aatcgtgcgt tccacccacg gtgttaactg tacaggctcc
     1801 tgtagctgga aaatctacgt taaaaatggt ctggtgacct gggaaatcca acagaccgac
     1861 tacccgcgca ctcgccctga cctgccaaat catgaacctc gcggctgccc gcgtggcgca
     1921 agttactcct ggtatctcta cagcgctaac cgcctgaaat accctctcat tcgtaaacga
     1981 ctgattgaac tgtggcgcga agccctcaag caacacagcg atccggtact ggcgtgggca
     2041 tcgattatga acgatccgca aaagtgcctg agctacaaac aagtacgtgg acgcggcggc
     2101 tttatccgct ctaactggca ggagctaaat cagctgattg ctgccgctaa cgtctggacc
     2161 atcaaaacct acggcccgga tcgcgttgcc ggtttctcgc cgatcccggc gatgtcgatg
     2221 gtttcttacg ccgccggaac acgttatctt tcactattag gtggcacctg tttaagcttc
     2281 tacgactggt attgcgacct gccgcccgcc tcgccgatga cctggggcga gcaaaccgac
     2341 gtaccggaat ctgccgactg gtataactcc agttacatca tcgcctgggg gtctaacgta
     2401 ccgcagacac gtacgccgga cgcccatttc tttaccgaag tacgctacaa aggcactaaa
     2461 accatcgcca ttacccctga ctactctgaa gtggccaaat tgtgcgacca gtggctggca
     2521 ccgaaacaag gcactgatag tgccctggca atggcaatgg gccatgtcat tttaaaagag
     2581 tttcatctcg ataatcccag cgattatttt attaactact gccgccgcta cagcgacatg
     2641 ccgatgctgg taatgctgga acctcgcgac gacggcagct acgttcccgg gcgcatggtc
     2701 cgcgcatctg acctggtgga tggactgggc gaaagcaata atccgcagtg gaaaaccgtg
     2761 gcagttaata ccgcaggtga actggtagtg ccgaacggtt cgattggttt tcgctgggga
     2821 gaaaaaggca aatggaatct ggaatccatt gccgccggta cggaaaccga attgttgtta
     2881 actctgctcg gtcaacatga tgccgttgca ggcgtggcct tcccctactt tggcggcatt
     2941 gaaaatccgc attttcgcag cgtaaaacac aatccggtcc tggtgcgcca attgcccgtt
     3001 aaaaacctga cattagccga tggcaacacc tgtccggtcg tcagcgttta tgatttggta
     3061 ctggcgaatt atggcctcga tcgcgggctg gaagatgaaa acagcgcgaa agattacgct
     3121 gaaatcaaac cgtacacccc agcctggggt gagcaaatta ccggcgtgcc gcgccagtat
     3181 attgaaacca tcgcccgtga atttgccgat accgcccata aaacgcatgg gcgctcgatg
     3241 attatcctcg gtgctggcgt caaccattgg tatcacatgg acatgaacta ccgtgggatg
     3301 atcaacatgc tgatcttctg cggttgtgtc gggcaaagcg gtggcggctg ggcacactat
     3361 gtcggtcagg aaaaactgcg cccgcaaacc ggctggttgc cgctggcctt tgcgctcgac
     3421 tggaaccgac caccgcgcca gatgaacagc acctcgtttt tctacaatca ttccagccaa
     3481 tggcgctatg aaaaagtctt tgctcaggag ttactttcac cgctcgccga tgccagtaag
     3541 tacagcggtc atctgattga tttcaacgtt cgtgccgaac gtatgggctg gctaccttct
     3601 gcgccgcagc tggggcgtaa cccgctcggg attaaagctg aagccgacaa ggccggatta
     3661 tcccccacag aatttaccgc ccaggcgctg aaatcgggcg atttacgcat ggcctgcgaa
     3721 caaccagata gcggcagcaa tcatccgcgt aatttgtttg tctggcgttc taacctgctt
     3781 ggctcctccg gcaaaggcca cgagtatatg cagaagtatc tgctgggtac ggaaagtggc
     3841 attcaggggg aagaacttgg accgacggag ggaatccaac cggaagaagt tgagtggcaa
     3901 actgcagcga ttgaaggcaa gctcgacctg ctggtgacgc ttgacttccg catgtccagc
     3961 acctgcctgt tctccgatat cgttctgccc accgccacct ggtacgaaaa agacgatatg
     4021 aatacctcgg atatgcatcc gtttattcat ccgctttctg cggcggtcga tccggcgtgg
     4081 gaatcacgca gcgactggga aatctacaaa ggtatcgcta aagccttttc gcaagtgtgc
     4141 gtggggcatc tcggcaaaga aaccgacatg gtattacaac cactgctgca tgactccccg
     4201 gcagagcttt cacagccgtg tgaagtgctc gactggcgca aaggtgaatg cgatctgatt
     4261 ccaggcaaaa ccgcgccgaa tattgtggcg gtggagcgcg actaccctgc tacctatgaa
     4321 cgctttacct cactcgggcc attgatggac aaacttggca acggcggtaa agggatttcg
     4381 tggaataccc aggatgaaat cgatttcctc ggtaaactca attacaccaa gcgtgatggc
     4441 ccggcacagg ggcgtccgct gattgatacc gcgattgacg catcagaagt gattctggcg
     4501 ctggcaccgg aaaccaacgg ccatgttgca gtcaaagcgt ggcaagcgct gggcgagatc
     4561 accggacgcg aacataccca tctggcgcag cacaaagagg acgagaagat tcgctttcgc
     4621 gatattcagg cgcagccgcg taaaattatc tccagcccca catggtctgg tctggaaagc
     4681 gatcacgttt cctataacgc gggatacacc aacgttcatg agttaattcc gtggcgcacg
     4741 ctgtcgggac gccagcaact ctatcaggat catccgtgga tgcgtgcttt tggtgaaagt
     4801 ctggtggctt atcgcccgcc tatcgacacc cgcagtgtca gtgaaatgcg ccagataccg
     4861 ccaaacggct tcccggagaa agcccttaac ttcctgacac cgcaccagaa atggggcatt
     4921 cactcaacct acagtgaaaa cctgctaatg ctgacgctct ctcgcggtgg accgattgtc
     4981 tggatcagcg aaaccgatgc ccgtgaactg accattgtcg ataacgactg ggtggaagtg
     5041 tttaacgcca atggcgcgct gacggcccgc gcggtggtca gccagcgtgt accaccgggc
     5101 atgaccatga tgtatcacgc tcaggaacgc attatgaata ttccaggttc ggaagtaacc
     5161 ggcatgcgcg gcggcattca taactcggtc acccgcgttt gtccgaaacc aacgcatatg
     5221 attggcggtt acgcgcagct ggcctggggc tttaattact acggcaccgt cggctcgaac
     5281 cgtgacgaat tcatcatgat ccgcaagatg aagaacgtta actggctgga tgatgaaggt
     5341 cgcgatcagg tacaggaggc gaaaaaatga aaatacgttc gcaagtcggc atggtgctta
     5401 acctcgataa atgtatcggc tgccatacct gttcggtgac ctgtaaaaac gtctggaccg
     5461 gacgcgaagg catggagtac gcatggttta acaacgtcga aaccaaaccg ggcattggtt
     5521 atccgaaaaa ctgggaagat caggaagaat ggcaaggcgg ctgggtgcgt gatgtgaatg
     5581 gcaagatccg cccgcgtctg ggcagcaaga tgggcgtaat aaccaaaatc ttcgccaacc
     5641 cggtggtgcc gcagattgat gattactacg aacctttcac cttcgactac gaacatttgc
     5701 atagcgcacc ggaaggcaaa catatcccta ctgctcgccc gcgttcactg attgacggca
     5761 aacggatgga caaagtgatc tgggggccaa actgggaaga actgctgggc ggcgaatttg
     5821 aaaaacgcgc tcgcgaccgc aacttcgagg ccatgcaaaa ggagatgtac gggcagtttg
     5881 aaaacacctt catgatgtac ctgccgcgcc tgtgcgaaca ctgcctcaat cccagctgcg
     5941 tggcgacctg cccaagcggt gctatctaca aacgtgaaga agacggcatt gtgctgattg
     6001 atcaggataa atgccgtggc tggcgtttgt gcattagcgg ttgtccgtac aaaaaaatct
     6061 acttcaactg gaaaagcggc aagtcagaaa aatgtatctt ctgttaccca cgaattgagt
     6121 caggccaacc caccgtgtgc tcagaaacct gcgtgggtcg catccggtat ctgggcgtgc
     6181 tgctttacga cgccgaccgc atcgaggaag cggcgagcac cgagcgcgaa gttgacctct
     6241 atgaacgtca gtgcgaagtg ttcctcgatc cgcacgatcc ctcagtgatc gaagaagccc
     6301 tgaaacaagg tattccacaa aacgtgattg aagctgccca gcgttcgcca gtctacaaaa
     6361 tggcgatgga ctggaaactg gcgttaccgc tgcaccctga ataccgcacc ctgccgatgg
     6421 tctggtacgt tcctccgctg tcaccgattc agtcttacgc tgatgcgggc ggtttgccga
     6481 aaagcgaagg cgtgctgcct gctatcgaaa gcctgcgtat tccggtgcaa tatctcgcca
     6541 atatgttgag tgctggcgat accggtccgg tactgcgggc gctgaaacgg atgatggcga
     6601 tgcgccacta tatgcgttca caaaccgtgg aaggcgttac cgatactcgt gccatcgacg
     6661 aagtaggcct gaacgtcgcc caggtcgaag agatgtatcg ctacctcgcc attgccaact
     6721 atgaagaccg ttttgtcatt ccaaccagtc atcgggaaat ggcgggcgat gcctttgctg
     6781 aacgcaacgg ctgcggtttt acctttggcg acggttgcca cggctcggac agcaaattca
     6841 acctgttcaa cagtagccgt atcgatgcca tcaacatcac cgaagtgcgc gacaaagcgg
     6901 agggcgaata atgcagatcc tcaaagtgat cggcctgttg atggagtatc cggacgagct
     6961 gttgtgggaa tgtaaggagg acgcgctggc gttgatccgc cgcgacgcac cgatgctcac
     7021 cgattttgca ctcgacctgc tcaacgcgcc gctgctggat aaacaggccg aatggtgtga
     7081 agtgtttgac cgcgggcgca ccacgtcgct gctgctgttt gaacatgttc atgccgagtc
     7141 ccgcgatcgc ggccaggcaa tggtggacct gctggcggag tatgaaaagg tcggtctgca
     7201 actgaattgt agggaactgc ccgattatct accgctgtat ctggagtatt taagcatact
     7261 tccggacgat caggcgaaag aaggattact caacgttgcg ccgatcctcg ccctgcttgg
     7321 cggacgctta aaacaacgcg aggcaccgtg gtacgcgtta tttgacgccc tgttgcaact
     7381 ggcaggaagc cctctatcaa gtgacagtgt cacaaaacag attcgcagtg aagagcgtga
     7441 cgacacccgc caggcgctgg atgcggtgtg ggaagaagaa caggtgaagt ttattgaaga
     7501 taacgccacg gcgtgtgaca gctcgccgct taatcaatat cagcgacgct ttagccagga
     7561 tgtcgcgccg cagtatgtcg acatcagtgc gggaggtggg aaatgattca gtatctgaac
     7621 gtcttttttt acgatatcta cccgtacttg tgtggcacgg tttttatcct tggaagctgg
     7681 ctgcgctacg attacgggca gtatacctgg cgtgccgcct ccagccagat gctggataaa
     7741 cgcgggatgg tgctatggtc gaacttgttc catatcggca ttctggggat cttcttcggt
     7801 catctgttcg gcatgttaac gccgcactgg atgtacgcgt ggtttctgcc ggtggcggta
     7861 aaacagcaga tggcgatgat cctcggcgga atatgcgggg tattgacgct gattggcggt
     7921 gcggggctac tggtgcgtcg tttaacaaat ccgcgtatca gggcgacgtc ttctggtgcg
     7981 gatatcctga tcctcgccat tttgttaatt cagtgcattc ttgggttaac caccattcct
     8041 ttttcagcac aacatccgga cggcagtgaa atgatgaagc tggtgggctg ggcgcaaagc
     8101 attgtcactt tccagggcgg tgcatctacg catcttgatg gtgtcgcctt tatattccgc
     8161 gttcatctgg tgcttggaat gaccatcttc ctgctcttcc cgttcacccg actggtgcat
     8221 gtctggagcg cgccgtttga gtactttact cgtcggtatc agattgtgcg ttcgcggcgt
     8281 taattctcac gcgggccgga cagcccttcg ctctccggcc acttgcaggc tatgatagtt
     8341 ctccagccac aggagaacat taatgaaacc gcaggtttac cacgtcgatg cttttacctc
     8401 tcaaccgttt cgcggcaatt ctgccggtgt tgttttcccc gccgataatc tcagtgaagc
     8461 gcaaatgcag cttatcgccc gcgagttagg tcattcggaa actgcttttc tgctgcacag
     8521 cgacgacagc gatgtgcgca tccgctactt tacgccaacg gttgaagttc cgatttgtgg
     8581 tcacgcgaca gtagctgcgc actatgtacg tgcaaaggtg ttaggtttag gaaattgcac
     8641 ggtctggcaa acatcgctgg caggaaaaca tcgcgtgact atcgaaaagc acaacgatga
     8701 ttatcgtatt tcgctggaac aaggtacgcc gggctttgag tcaccgctgg aaggtgaaac
     8761 acgtgcggcg attatcaacg cgcttcatct cactgaagac gatattctgc caggcttgcc
     8821 aattcaggtg gcaaccacag gacactcaaa agtgatgatc ccactgaaac cggaagtgga
     8881 tatcgacgcc ctttcgcctg accttaatgc gctgaccgct atcagcaaac agattggctg
     8941 caatggtttc ttcccgttcc agatccgccc agacaaaaac gaaaccgacg gtcgtatgtt
     9001 ctcgcctgcc attggtattg tggaagatcc ggtgactggc aatgccaatg gcccgatggg
     9061 tgcatggtta gtacatcaca acgtattgcc ccacgatggc aacgtgttgc gtgttaaagg
     9121 ccatcagggg cgcgcattag ggcgtgacgg cgtgattgaa gtgacggtga caattcgtga
     9181 taaccaaccg gagaaagtca ccatttctgg cgtggcggtg attttgtttc atgccgaatg
     9241 ggcaattgaa ctctgaaacc gttgcctgat gcgcttcgct tatcaggtct acaaccgggc
     9301 taatgtaggc cggataaggc gtttacgcca catccggcaa cctgacataa attattttcc
     9361 cgcctccggg tgcgtatcaa acgccgccat caccagcgct aactcatcca cagtaaagcc
     9421 atgtttcgca tcatctacgc ccagaccaaa ctgttcctgc atcacggcat ataacgacgc
     9481 cacatctggt agatttcgct gctcaactgc gtgcccattt tcataatggg taaaatgaaa
     9541 attggtcagc gttagcttgc caccgtccgg caaatggcga cacatcagca aatgatggcg
     9601 aaaatgcgac tgcggccagt gcgccgacca gaaattgccc atcacataat cgctttgttg
     9661 ctgctcgcag agatcaaaac ggtacatcga ctgccagtgc tgatgatgat taaactgcaa
     9721 cacccaatca tcaccctctt gcaacaaccg atactctccg tgtggcgtgg tttgcatgat
     9781 atccggcatt aaacgaatcg gcgcggttag cgtctgccca ccgaaaccga catcagcaat
     9841 ccatttttcc ccttccagtt ccaccaacag caaacgatgg gtgcgcggcg gtaatgctgg
     9901 cggatttgat aacactacgc gccctaacaa gctgcgaacg ttaaacccca gctcgcgtaa
     9961 cacccgctca aacacgccat tctgctcaaa acagtaacca ccacgacggg ctatcaccag
    10021 tttctcttcc agcgattgat catcaagctg tatttccctc ggcagcaaaa cgtcgaggtt
    10081 ttcaaacgga atggtgcaat tgtgtttcag gtgcaatgca cgaagcgtat caatattggc
    10141 cgcagcagct cccgaccagt taatacgggc aaaataatga ttcagaatgg gcgtcatcag
    10201 gcgtttccct tagtggtttt ctcaacttat aacctggaat agacgccgca ccggctattt
    10261 tgtgcgatcg cggacatgca tcacgttcgc aaatcgccaa tcccgttaat ttcgttactt
    10321 attgcgttac gagctacgct tttaaaacac ccttcatagc gaggcgatat cgtgagccaa
    10381 ttcattcccg tcgaattaca ccatgccagc cgtctgttaa atcatggccc aactgtcttg
    10441 atcaccagtt tcgatgagca atcccagcgg cgtaacatta tggctgcagc ctggtcaatg
    10501 ccggtggagt ttgaacctcc acgcgtggcg attgtggtag ataagtcgac atggaccagg
    10561 gagttgattg agcgtaacgg taaatttggc atcgttatcc caggcgttgc agcgactaac
    10621 tggacgtggg cagtgggaag tgtatctggg cgtgaagagg ataaatttta ttgctatggc
    10681 attccggtgg tgaaaggtcc ggtgcttggt ttgccacttg ttgaagagaa atgtctggcg
    10741 tggatggagt gccgattgct accagcgact tccgcgcaag agaaatacga cacgctgttt
    10801 ggcgaagtgg tatcagcagc ggcagacgca cgggtatttg tcgaaggccg ctggcagttt
    10861 gatgatgata agctcaatac gttgcatcat ttaggcgctg gcacgtttgt taccagcggc
    10921 aagcgagtca ccgcgggcta aagaatcata tgcagcaacc aagttgctta agcaatgttt
    10981 caatatgagt taaaagcata ttttatctca cgattgcata tttttagtga agattaattt
    11041 atcctcacca acatcatagt aattgcgtat tatagcttca ttgataaagc cctgttatgt
    11101 ataaaatgcg cgggcggcac taaaatcgac atagctggag gtctctacta aaagtagtct
    11161 tgcctgttta attgtgagct ccttttctat ttcgttaatc agcagatgtc ccactccctg
    11221 gcgctgatgg gaaggactga tccagtgcat taataaattc cagacatcat ttgtcatcac
    11281 ttcaggagcg caataggcaa tccctgccag tcctaagtgt tctgcactaa accataaatc
    11341 ggcagattga cctgagaaat aatgtgttag tgttttttgt acatgatgaa ggccatcttg
    11401 attaaattgt ccactggcaa gcagcatatc cagtaatggt tcaacatctg aaggtttgac
    11461 tggtcgtata atcatggaat tccttatcta ttgatgaatg tttgaaaatt gtttgcgtgg
    11521 aacatggtta atctgagcca atatttttga gaaagaatag atgggtattt atataagcaa
    11581 agaagccatc tctttaaata tcaattaaag tataacatta acaccagact tcattgcgac
    11641 aggacacgac aaagcacttt tgcggttttt tcagggtgca attacaaaaa ttgaatccct
    11701 tatggactaa tgtgagaaaa ttccggggca tgactatttt gatatagtgg acaatcaagc
    11761 gtctcgaatt aacaatgact tttcagatgc taaaccatgc aattggaagt atgtcggata
    11821 taggacaaca ttaggaaatc ggacagaaaa ttcatcataa taaaatgaca acttatctct
    11881 ttctctcact ttctgagaaa tttcacaaaa gta
//
LOCUS       DAZKJR010000150        11901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKJR010000150 DAZKJR010000000
VERSION     DAZKJR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.151"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..915
                     /locus_tag="ACHOWS_004261"
     CDS             <1..915
                     /locus_tag="ACHOWS_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0049754.1"
                     /translation="SDKLREKIEQAVEELGYIPNRTAGALASGHSDTVAVLVPSLTDK
                     TSSRFMQSLQQVLNKNEFQLLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKT
                     HQILEKTNIPTINVVGSPFSPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDN
                     RLQRQQLNGWHKAMLSYYQNSDLVITMPGAASLQLGRYALNEILLRQPELDAVICSHE
                     EIALGIMFECQRRLLKIPGNIAVACLDGSDSCDQTHPTLTSIRIDYKKMGTETGKLLI
                     GLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1118..1798
                     /locus_tag="ACHOWS_004262"
                     /pseudo
     CDS             1118..1798
                     /locus_tag="ACHOWS_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1815..2123
                     /locus_tag="ACHOWS_004263"
                     /pseudo
     CDS             1815..2123
                     /locus_tag="ACHOWS_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2226..3332)
                     /locus_tag="ACHOWS_004264"
                     /pseudo
     CDS             complement(2226..3332)
                     /locus_tag="ACHOWS_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4099..6147
                     /gene="ireA"
                     /locus_tag="ACHOWS_004265"
     CDS             4099..6147
                     /gene="ireA"
                     /locus_tag="ACHOWS_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFQ0049755.1"
                     /translation="MKNKYIIAPGIAVICSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
     gene            6821..11140
                     /locus_tag="ACHOWS_004266"
     CDS             6821..11140
                     /locus_tag="ACHOWS_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFQ0049756.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDESSQGYDVHG
                     DNWKSIEIGTTQEVKASPDKNINFDHNSPVAEGRNSIAIGTSAEAGKEMTDKMAGEAI
                     AIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNNYSSSEAS
                     GDGSIVVGSRSTAAGDLSVSLGSISSANGTAANALGATAEANGDMATAVGFNALANAS
                     NALAVGSQSSAGGEESLAIGSQSTTGKKSTVALGQGATASAAEGNVAIGADSVDKAAT
                     PVSGATIKLKNGGTIEYRGFAGDKAASVVSVGDAGKERQIVNVGAGAVSEKSTDAING
                     SQLYAIAKTLKDDLDAINPTWKLKDSTGKEKEVGSKNGGTTGNEVTVTGKGDIDVTVD
                     GNGLSIDGTKLAENIKKDAIKIKTGDGKTLTKKLGEEVEFNDAGPIKTKINNNKLEIT
                     AETASFTPDGNGGNGKITVSNGDDDKLVTAKNIADAINQSGWKIKAGSENGGKVEGDN
                     KEELINPGDTVTLKAGKNLSVKQSNGDFTFSVVDTPSFSSVQLNGKGNNGNVTLSSEG
                     NKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAASVADLKTAIDGVST
                     SMQGAGFALKDSKGQEVKQSLGQAIKVSGDSNITTTAITNGDKGLKISLNNDLSVGSN
                     KAPGSIKVNGTDGKEAVSLNGQDGTIKLTSAPAANGKSASATIAVNAGKGGLTDAVGT
                     TKTRLTYTPDGKSPAEELATMNDGLSFEGDDGKIIHKKLNEKLTIKGGAGDNEDVTDS
                     NIRVDANEKGDGLLVRMTRNLRDLASAVFGTENGPTTRIDNAGITITTPAAGGGQGNT
                     VSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDSSAALVNT
                     GFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLADDGSVSVGG
                     TRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQATAATT
                     WALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEIDVTGIPVEYTDSQG
                     NPLVNIGGRFYTQTEDAATGKLTMTPAEPARVRISSEQPLVLTNVAAGNVSPVSTDAV
                     NGSQLASAARIAGGDNVVWKDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVADA
                     VNMLNREGTKYFKAASSGQAAKAEGRDSIAVGQGAVSRGQNSIAMGNGAEVTRQAAAG
                     SVAVGSHARAGRANTGTYALNSQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVLSAN
                     STDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAMSGI
                     PQAYLPGKSLMGLGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAGVG
                     WQW"
     gene            11165..>11901
                     /locus_tag="ACHOWS_004267"
     CDS             11165..>11901
                     /locus_tag="ACHOWS_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFQ0049757.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRP"
BASE COUNT         3359 a         2495 c         3077 g         2970 t
ORIGIN      
        1 tctgacaagc tgagggaaaa gatagaacag gccgtagaag agttgggtta tattcccaac
       61 agaactgctg gcgcccttgc atctggtcac agtgatacag tcgccgtact ggttccttcc
      121 ctgacagata aaaccagctc ccgctttatg cagtcgttgc agcaggtgct taataaaaat
      181 gaattccagc tactgcttgg gtgtcatgag tataatcagc tcaaagaagc tgaaatcctg
      241 atgacgcttc ttcaaggtaa cccggcggcg ttagttattt ttggttctca gttggctgat
      301 aaaactcacc agatactcga gaaaacaaat atacctacca ttaacgttgt cggttcccct
      361 ttcagtccgg ctcaaattac catcgaaaca gcattttttg aagcatcaca taaactgacc
      421 gaacatctgc tggaacaggg ttataagaat atcggattta tcggtgcgca tatggacaac
      481 cgcctgcaac gtcagcaact gaacggctgg cataaagcaa tgctgagcta ttaccagaac
      541 tctgatcttg ttatcaccat gccgggtgcc gcaagtttgc aattggggcg ttacgcatta
      601 aacgagatat tactgcgaca accagagctg gatgctgtga tttgcagcca cgaagagatt
      661 gcgctgggta tcatgtttga gtgccagcgg cgtttgttaa aaatccctgg aaatatcgct
      721 gtagcttgtc ttgacggttc agacagttgc gatcaaacac acccaacctt gacatccatc
      781 cgtattgact acaaaaaaat ggggacagag acgggtaagc tgctgattgg tctattgaat
      841 aataatcatg atgaatcaga agagtcgcgt atcgtgcaat tcaactacca gatagaatta
      901 cgtcagagta catgagctca aaaggggatt attgcaaagt tagctattgt ggtagggata
      961 gccgtttccg gctattcccc gcgcagagcc ggatgtgtgc tgctaacgca tcctgtcaac
     1021 gacggatgaa aagttatcca cttatatctc caccaacggc ccaatattgt atcgttcgtg
     1081 acacatccat ttctacttca ggtatttggc gtagtagcgg gtgctaaaaa tttgccgcaa
     1141 aaccgcaata tcagcccgca gaggcgctct ccagattatc cattcctata ttatctctgc
     1201 accggatgaa ttactcgaac aactcagaaa tatgacacat atgcaactta tcaggactct
     1261 gggatcctgg aggcctgatg ccagtgaata cagtaatgtt accaacgttt atcgcattac
     1321 attgaaatcc cttgttcgac gctatttcga gtttcatgat gaaatcacca acttggatgt
     1381 catgattgcg gcaattgttg atgaactggc acctgaactg attaaacgta atgctgttgg
     1441 atacgaaagc gctttgcagt tgttgatctc tgccggagac aatccctaac ggttaagaac
     1501 cgaatcaggt tttgcggcgt tgcgcggtgt cagccagttt cccctatctt ctggaaaaac
     1561 gaaccattac cgacttaatc ggggtgggga gcgtgttgca aatagtgcac tttacatcat
     1621 cactatagga tgtttacaaa ctgacgataa aacaaaggaa tatgtctcca ggcgagtagc
     1681 cgaaggacat acaaaaatgg aagcaatacg ctgtctgaag cgctatatct cacgcgaagt
     1741 ttatacatta ctacgaaatc aaaacaggca tgttaactgc atcccgataa cgtcttgact
     1801 cttagaaggg cgtccaaggc agtcattatt cgagcaggca gttccggcag ttattgtgtc
     1861 gataccagat caggcagagt atgagccggc gcggaaaatg ctgggataac agctcaatgg
     1921 aacgcttctt caggagtctg aagaacgaat ggatgccggt ggtgggttac gtaagcttca
     1981 gcgaggcagc tcacgccata acggacgata tcgttggata ttacagcgca ctaagaccgc
     2041 acgaatataa cagtgggtta tccccaaacg aatcggaaaa tcgatactgg aaaaactcta
     2101 actcggtggc cagtttttgt tgaccacttc agccatcgcc agacgctaac atgagttact
     2161 cttgttctgg gctacgtcac cttcagggaa ctggttatcc tcattcttta tttgtgacat
     2221 tgtggttatt gtgcgacaaa catggctatc ccgccccata tcccagataa aaccacctgc
     2281 tcacgtgcga cagcgacaat tgtaatattg acattctttc ctttggcctg aagttttcga
     2341 gacctcttgc aaagcctgag ttgagcatcc catgctctgt caatgacggg gcggggtaaa
     2401 ttttcctgat tttttgtatt gccggactga tgcgtgtcgg atgccggtaa ctccacgctg
     2461 cctcaaccaa tagctttctg gcatagctgt ttccggcttt ggtaatgctg ccctgatgtc
     2521 tgcttccacc cgatgaatat tctgatggcg tgataccaaa ccatgacatc agttgtcgtg
     2581 gatgttcaaa acgggtcaga tcacccagtt cagaaattaa tccaaccgca gtaataaact
     2641 gaatccctcg catggcttgc tgcgcctcta ctaccggata cagcctccat tcggtaacgg
     2701 cctccttcgg gcggattcca gccgttcaca ttgtgcctgc cggtcttcta ttgtacaacg
     2761 gtgttcgtca aaagctaatt gtcgccaggg actttcgaat gagtatttgc tgagccagtg
     2821 tcggtgtgca gggccccaat ctgctcgccc gacataatgg accccatgaa ccagaagaaa
     2881 cgatttcaga cgttgccttg catgccgtaa atcatcgcgg gcagatgccc atgcccgggc
     2941 caaatcccgg aatgcctcat cttcaacgcc ggatacgtag acagcagaaa gatctcctgc
     3001 acgcagtgaa cgcacaagtc tgatggcatc acggtgtcgg ttttaccccg ctcccccggt
     3061 tttttcggga taagtgaggg ggcgcagacc atgcagtcaa aaccggattt caccagccga
     3121 cgataaagtc cataaccaca gggtcccgct ttataaacga tactgacctg cgatgacttt
     3181 gacctgatac gttgataaag attatgaatg tcagtaggtg atgtgctaat tttacccatc
     3241 agttcaacag gttctgaatt gatagcatag gagacagtaa tcgattcttt gtgaacatct
     3301 aattcgacgt acaacatgtt attgttgttc atgctggcct ccgtggtgga aattccaggg
     3361 gggtagcaac agctcaccca tgcggctctg acaacgatgt taacccgcat taaatatccc
     3421 acgttggaca gcatctgtct cacggaaagt catactgcct tacgtgcata tttcacctta
     3481 cgccttcact gtctgtcatc ggaagtactg atttatattg gggctttgca cttactgtca
     3541 ttcacgggaa aagatggtgg tgaacagtac gatactgtct tggctgttgc cgagggaaga
     3601 ttcaggagtg gtaagggggt tagtggtgta agatgagtct ggagctctga ctggtcagtg
     3661 acgcgaaact caatctgcca ggataaactc ccccttgctt ttgcgcagaa gcagtgggcc
     3721 ttggttcgct tatgtcctgc atctacaagc gaatgactga ccggagactc ccatatctgg
     3781 tcagtcgccc atttttattt tccaccacat cggacaagca taccgtaata aacagcacca
     3841 gtctcctgtt cactcttgct tgatttactg gaatgctgtt tatggagata tccggttgaa
     3901 agattttacc tcttcgacag gactataaat tatacagtaa ggagtccctc gtagaaaggg
     3961 gctgattctc atcaagtcgg acaggttcaa agtgttacct gaatgagcta aatgttattt
     4021 accttccttt ctgttcattg taaatttttt cttgctaacg caaatcatta tcattacgat
     4081 tttagaaaag ggaaatttat gaagaacaaa tatatcattg ctccgggcat tgccgtgata
     4141 tgttctgcag tcatatcatc aggttatgcc agttctgata aaaaagaaga tacgcttgtt
     4201 gttactgcct ccggcttcac tcagcaactc agaaatgcac cggccagtgt ttcagtcatt
     4261 acttcagagc aactgcaaaa aaaaccggtt tcagacctgg tcgatgcggt aaaagatgtt
     4321 gaagggatta gtatcaccgg tgggaatgaa aaaccggata tcagtatacg tgggttaagt
     4381 ggcgattata cattgattct ggtcgatgga cgacgtcaga gtggtcggga atccagacca
     4441 aacggcagcg gcggttttga agccggattt atccctcctg tggaagcaat tgagcgtatt
     4501 gaagttatac gtggccctat gtcttccctg tatggttccg atgctatcgg aggagttatt
     4561 aatatcataa ccaaacctgt taataaccag acatgggatg gcgtacttgg gcttggggga
     4621 attattcagg aacatgggaa atttggtaac tcaaccacga atgacttcta cctgtcaggc
     4681 ccattggtta aggataaact tggtcttcag ttatatggag gaatgaacta tcgcaaggaa
     4741 gatagtatct ctcagggaac accagcaaaa gataataaga atataacggc aacactccag
     4801 tttactccga ctgaaagcca gaagtttgtt tttgaatacg gaaaaaataa ccaggaacat
     4861 acattaacac ctggtgattc tcttgatgcc tggactatgc ggggaaatct taaacaacca
     4921 aacagtaaaa gagaaacaca taactcacgt agtcactggg tagcagcatg gaatgcccag
     4981 ggcgaaatac tgcatcctga aattgctgtt tatcaggaga aagttattcg tgaggttaaa
     5041 tctggtaaaa aagataaata taatcattgg gatcttaatt acgagtcaag aaaaccggaa
     5101 ataaccaaca cgatcataga tgcaaaagtg acggcatttc tgccggaaaa tgtgctgacc
     5161 atcggaggtc aatttcagca tgcagaactc cgtgatgact cagccacagg taaaaaaacg
     5221 acagaaacac agtctgtttc agttaaacag aaagctgttt ttatagaaaa tgaatatgct
     5281 gcaacggatt ctctcgccct gactggaggg ctgcgcctcg ataaccatga aatctatggc
     5341 agttactgga atccaagatt gtatgctgtt tataacctga ccgataatct cactctcaaa
     5401 ggagggatcg caaaagcatt tcgggcccct tcgattcgtg aggtcagtcc tggatttgga
     5461 acactgacgc agggtggtgc ctctattatg tatggaaaca gggacctgaa accggagacc
     5521 agtgtaaccg aagagatcgg tattatttat aataatgaca gtggtttttc agcaagcgcg
     5581 actctgttta atacagattt taaaaataag ctgaccagtt acgatatagg cacaaaagat
     5641 ccagtcactg ggctaaacac ttttatttat gataatgtgg gtgaggcaaa tatcagaggt
     5701 gtggagcttg caactcagat tcctgtgtat gataaatggc gtgtatctgc aaactataca
     5761 ttcactgact cccgtcgaga aagcgatgac gaaagtctca atggcaagtc gctgaaaggg
     5821 gaacctctgg aaagaactcc cagacatgca gccaatgcaa aactggaatg ggattacact
     5881 caggatatta cattttattc atctctgaat tatacaggaa aacaaatctg ggcagcacaa
     5941 agaaatggtg ccaaggttcc ccgtgttcgt aatggattca cttctatgga tattggtatg
     6001 aattaccagt tcctgccagg cacgctgatt aattttgccg tacttaacgt cacagacaga
     6061 aagagcgaag atatcgatac cattgatggt aactggcagg tcgatgaagg acgtcgttac
     6121 tgggctaatg taagaatatc cttctgagag aatatagata acctgaggcc gtgcgcctca
     6181 ggtaccagta tagtaatcag taacgtcagc ttccaggttg taatgctttt aattttgata
     6241 aaaataaact taataaatta catttgttaa atattgataa ttttaatttg gttttattac
     6301 tccagttata ctcacactct atctttacac aattcatcac cattgcacct ccatagcctg
     6361 ttgatttaaa agatttaatt tcctgtgaaa caatcaggcc tgtatgaacc tgattatacc
     6421 acataatctt ttaagttatt taaatttaac tctcgggctt ttctgtcagt aaaatcagaa
     6481 aaattgcagg atttcgtttt tttgctgcga agtgaaatgt atctcatttt tactgccttc
     6541 gccagtgcgt caggcaatgg caggtagtgt acctgcattc tgctgtaacg cgtaatgtcg
     6601 ggtagtttac ttaaacttaa tatgttttat gttcgcatta ttataaatca ctttattgcc
     6661 ttttgtctgc tttttaatgg ttcccgtttt gttcacttct ggctttataa aaaatggatg
     6721 ctgaagaaat gaaggcagtc cgccttacat aatattcatg catgattttt atgtcattcc
     6781 gactgagtgg aatgaaaata aaggagaaaa gagggtatat atgaacctca gaaaaaaaca
     6841 tatatgcata gccatatcac ttgttcttaa tccgttccct gcatcactgg cagcacaaaa
     6901 cactaatgta gttgaagttg atgaaagcag tcaggggtat gatgtgcatg gagataactg
     6961 gaagagcatt gaaataggga caacgcagga agttaaagca tcccccgata aaaatattaa
     7021 tttcgatcat aattctcctg ttgcagaagg acgtaactct atcgcgattg gtaccagtgc
     7081 agaggctggt aaagaaatga cggataaaat ggcaggggaa gccatagcta ttggtgccaa
     7141 ggcgaaagca actaaagaac agtcaattgc aataggtggc gataccaaag ctaccggatg
     7201 gggggctatt gttattggtg gcgatgattt aacccctttg catggtgaca gattcggtga
     7261 taaagaaatt ccgaataatt actcgtcatc tgaagcatca ggagacggaa gtattgttgt
     7321 gggctcaagg agcacagctg caggagacct gtctgtgtcc ctgggctcaa taagctctgc
     7381 caacgggacg gctgctaatg cactgggagc cacggcggaa gctaacgggg atatggcgac
     7441 tgcggttggt tttaatgcgc tggctaatgc cagtaatgcg ctggctgttg gtagtcagtc
     7501 ctctgccggc ggagaggaat ccctggctat agggagtcag agtacgacgg gcaaaaagtc
     7561 cacggttgct ctggggcagg gagctacagc ctctgccgcg gaggggaacg tggccatcgg
     7621 tgctgattca gtggacaagg cagcaactcc ggtttccggc gctaccatta agttaaagaa
     7681 tggcgggact attgaataca ggggctttgc cggagataag gctgcatcag tagtatctgt
     7741 tggtgatgcg ggtaaagagc gtcagattgt taacgtgggg gcgggtgctg tatctgagaa
     7801 aagtacagat gccattaacg gtagtcagtt atatgcgata gcgaagacac tgaaagatga
     7861 ccttgatgcc attaatccga cgtggaagtt aaaagacagc acaggaaaag aaaaagaggt
     7921 cggcagtaaa aatggcggga ctacaggtaa tgaagtgacc gtcaccggta aaggggatat
     7981 tgatgttact gttgatggaa atgggctgag tattgatggc acaaagcttg ctgaaaacat
     8041 caaaaaagat gccatcaaga ttaaaacggg agatggtaaa acactaacga aaaaacttgg
     8101 tgaagaagtt gagtttaatg atgccggacc aataaaaaca aaaattaata ataacaaact
     8161 tgagataact gcagagacag ccagttttac gcctgatgga aatggtggta acgggaagat
     8221 tacagtaagc aatggtgatg acgataagct tgttactgcg aaaaatattg ctgatgccat
     8281 aaaccagtct ggctggaaaa ttaaagctgg cagtgaaaat ggtgggaaag ttgaggggga
     8341 taataaagag gagctaatta atcccggtga tacagttaca ctgaaagccg gtaaaaatct
     8401 cagcgtgaaa caaagtaacg gtgattttac attctctgtt gttgatactc cgtcattcag
     8461 cagtgttcag ctgaatggaa aaggaaataa cggaaatgta acgctctcat ccgaaggtaa
     8521 taagctgaca ttgtcaggcg gtaatggtgc cggacaaaat ggtgttgtta tcagtggtgt
     8581 ggcaagtgga ctggaaggta aggaactttc gacgttaacg aatactgata aagagatgaa
     8641 aaatgccgcc agtgttgcag acctgaaaac ggcaattgat ggtgtgtcca ccagtatgca
     8701 gggagcaggt ttcgcactga aagattctaa gggacaggaa gtgaaacagt ctcttgggca
     8761 ggcgattaaa gtatccgggg acagcaatat caccaccaca gcaatcacca atggggataa
     8821 ggggttaaaa atatccctga ataacgacct gagcgtgggg agcaataagg ctcccggcag
     8881 catcaaagtt aacgggactg atggtaagga ggctgtgagt ctgaatggtc aggatggcac
     8941 gattaaactg acatccgcgc ctgctgcgaa cggaaagtct gcgtcagcaa ccattgctgt
     9001 taatgcaggc aaaggtggat taacagatgc tgtcggtacc acgaagacac gtctgactta
     9061 taccccggat ggaaaatcac cggcagaaga gctggcgacc atgaatgacg gtctttcttt
     9121 tgagggtgat gacggaaaga ttattcataa aaaactgaat gaaaaactga ccatcaaagg
     9181 tggtgctggt gataatgagg atgtaacaga cagtaacatt cgtgttgatg caaatgaaaa
     9241 gggagatggt cttctcgtcc ggatgaccag aaacctccgt gatttggcca gcgcggtttt
     9301 tggtacagaa aacggcccga caacccggat tgacaatgca ggcatcacaa tcacaacacc
     9361 tgctgcgggc ggtggtcagg ggaatacggt ttcccttacc ggctccggcc tgaataacgg
     9421 tggaaatcag ataaccagtg tggccagtgg tctgaacggc actgcgcttg ctgacgccgg
     9481 tggagatgtt ctgaataatg cggccaatat cggtgacctg aaaaatgctc tccgtgacag
     9541 cagtgcggca ctggtgaata ccggctttgg tctgcaggcg gatgacggaa gcaaggtgaa
     9601 cacgtctctc gggaagcata tcacggttgg tggtgacggg cagaatatca gcacgactgt
     9661 tcagaacggg agcctgacgg tcagactcgc tgataacatt cgcctggcag atgacggcag
     9721 tgtttctgtc ggcggaaccc ggatggataa aaacggactg accatcaagg atggcccgtc
     9781 agtgactgtg aacggtattg atgcggctgg ccgggttatt tccggcgtga aagacggggt
     9841 gaatgaccag gacgccgtta acgtttcaca actgaaccgt gaaatcagtc aggcaacggc
     9901 agcaacgacc tgggcactga agactgaatc cggtgatgag gctgttgctg tttcttccca
     9961 gacagttgaa gtccgccacg gacagaatac ccgggtttca gccatcagta aggatgataa
    10021 gggaaattac agctatgaga ttgatgtgac ggggattccg gtggagtaca ccgacagcca
    10081 ggggaacccg cttgtcaata ttggtggtcg tttttacaca cagaccgaag acgctgcgac
    10141 cgggaaactg acaatgacgc cggctgaacc agcccgtgtc agaataagca gtgagcagcc
    10201 tcttgttctg acaaatgtgg cggccggaaa tgtgtcaccg gtatccacgg atgcggttaa
    10261 cgggagccag ctggcatctg cagccagaat tgctggcggt gataatgttg tctggaaaga
    10321 cggaaaagca gcgctggctc ctgacacctt cagtgagctg acaactgcag gtggcggtaa
    10381 gacaacggtg aatggtggtg tacataaaac accggataat gtggctgatg cagtgaatat
    10441 gctgaaccgt gaaggcacga agtattttaa agctgcctct tccggtcagg cagcaaaagc
    10501 ggaaggacgt gacagcattg ctgtcggaca gggggccgtt tcccggggac aaaacagcat
    10561 tgccatgggt aatggtgctg aagtcacgcg tcaggctgct gccggctctg ttgcagttgg
    10621 cagccatgcc cgtgctggcc gcgcgaatac cgggacttat gccctgaaca gtcaggctgt
    10681 tgcgggacgt accggaagtg atactgcggt ggtttctttt ggtcagccgg gacaggagcg
    10741 tcagctgcag tcagtggcac cgggtgtgtt gtctgcgaat tcaacggatg ccgtcaatgg
    10801 cagtcagttg catgcaacta accgtcaggt tgccggaaat acacaggcga taaacactct
    10861 cgggaataaa ttcagtcagc tgagctaccg ggtggaagag cttaaccggg atatccgtgg
    10921 tgttggtgca tcggctgcgg ccatgagcgg gatacctcag gcctatctgc cgggtaaaag
    10981 tctgatgggg ctgggtgtcg gtggatatgg tggtgaaagc gccattgcaa taggtgtgtc
    11041 ccgaatctcc gataacggta agatgataat gaaactgaat gcaggtcaga acacccgggg
    11101 taatttctcc gtcggtgccg gtgtgggctg gcagtggtaa cagggaaaag caaggagaaa
    11161 tgtaatgtta tttagtcgaa tgatgatgac gggtgtgctg tccctgatgc ttgcaggatg
    11221 tggaaatctg agtcatgtca cggatgaagg aacgacggaa gaagtggtat ggccttctcc
    11281 ggagaaggtg atgtttaatc atgacggaac acagaaaggt tcgtggccac tgccagaaaa
    11341 gttacgtcag ataaaggcgg gtatgaataa ggaccagatt tatcagctgg cggggagacc
    11401 acactttgct gaagggatgt ctggtgtaag ggaatgggat tacctgttca actttaccgg
    11461 accggagggg gattatacct gccagtacaa gatactgttt gaccggaaca tggatgcccg
    11521 ctctttctac tggaagccgg aaaactgtct gccggaggca gagacagcag aatacaggga
    11581 gtcagtcccc ggtgatatat tatttgattt tgattcatgg actctgacag agagggggcg
    11641 tgatgcactt tcccgtgtgg cagagaagtt tcaggcgggt catgttgtcg gggtgaagat
    11701 agaaggattt acggaccatc ttggcgggca ggcgtataac cggctcctgt cagagaaacg
    11761 ggcggaatca gtcagggggt ggtttattgc ccggggattc agggctgaaa ggatacatgc
    11821 aacgggccgt ggatatgatg caccagtggt gttctgcccg gatgtggagg gcgaagcact
    11881 gaaggaatgt ctgcgcccca a
//
LOCUS       DAZKJR010000151        11782 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKJR010000151 DAZKJR010000000
VERSION     DAZKJR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.152"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..814
                     /locus_tag="ACHOWS_004268"
     CDS             74..814
                     /locus_tag="ACHOWS_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFQ0049758.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(935..2914)
                     /locus_tag="ACHOWS_004269"
     CDS             complement(935..2914)
                     /locus_tag="ACHOWS_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFQ0049759.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNAYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHTVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHPEVG"
     gene            complement(2925..4325)
                     /locus_tag="ACHOWS_004270"
     CDS             complement(2925..4325)
                     /locus_tag="ACHOWS_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0049760.1"
                     /translation="MTSTPITAADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            4551..5366
                     /locus_tag="ACHOWS_004271"
     CDS             4551..5366
                     /locus_tag="ACHOWS_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0049761.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(5398..6093)
                     /gene="araD"
                     /locus_tag="ACHOWS_004272"
     CDS             complement(5398..6093)
                     /gene="araD"
                     /locus_tag="ACHOWS_004272"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0049762.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDEARQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6087..6947)
                     /locus_tag="ACHOWS_004273"
     CDS             complement(6087..6947)
                     /locus_tag="ACHOWS_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0049763.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(6940..7602)
                     /gene="ulaD"
                     /locus_tag="ACHOWS_004274"
     CDS             complement(6940..7602)
                     /gene="ulaD"
                     /locus_tag="ACHOWS_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0049764.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(7599..9095)
                     /gene="lyxK"
                     /locus_tag="ACHOWS_004275"
     CDS             complement(7599..9095)
                     /gene="lyxK"
                     /locus_tag="ACHOWS_004275"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFQ0049765.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPLVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTTQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRVHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(9099..10085)
                     /gene="yiaO"
                     /locus_tag="ACHOWS_004276"
     CDS             complement(9099..10085)
                     /gene="yiaO"
                     /locus_tag="ACHOWS_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFQ0049766.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            complement(10098..11375)
                     /gene="yiaN"
                     /locus_tag="ACHOWS_004277"
     CDS             complement(10098..11375)
                     /gene="yiaN"
                     /locus_tag="ACHOWS_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFQ0049767.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(11378..>11782)
                     /gene="yiaM"
                     /locus_tag="ACHOWS_004278"
     CDS             complement(11378..>11782)
                     /gene="yiaM"
                     /locus_tag="ACHOWS_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFQ0049768.1"
                     /translation="IILRYGFQTSILSVDELSRYLFVWLTFIGAIVAFMDNAHVQVTF
                     LVEKLSPAWQRRVALVTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAAC
                     LPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
BASE COUNT         2823 a         3155 c         3143 g         2661 t
ORIGIN      
        1 tcttgcaccg gagaaattgt aagcattact atgccgactc ttttttatct tattttaaaa
       61 tggaataccg gacatgttaa ttaaccgcaa tattgtggcg ttatttgcgt tgccttttat
      121 ggcaagcgca actgcttctg aattatccat tggtgctggt gcggcttata atgaatcgcc
      181 ttatcgcggt tataatgaaa atacaaaggc aattccgctg attagttatg aaggtgatac
      241 tttttatgtt cgtcagacca cgttaggttt tattctgtcg caaagtgaaa aaaatgaact
      301 tagcctgacc gcatcctgga tgccgctgga atttgaccct accgataatg acgattatgc
      361 catgcaacag cttgataagc gtgatagtac ggctatggcg ggggttgcct ggtatcacca
      421 cgagcgttgg ggaaccgtga aagcctctgc ggctgcggac gttctggata acagcaacgg
      481 ctgggtgggg gagctatcgg tattccacaa aatgcagata ggtcgcctgt cgctgacacc
      541 tgcgctgggc gttctctatt atgacgagaa tttcagtgac tattactatg gcatttcaga
      601 gagtgagtcc cgtcgtagcg gtctggcaag ttattccgcg caggatgcct gggtgcctta
      661 tgtcagcctg acggcaaaat acccgatagg agagcacgtc gtattgatgg cgagcgccgg
      721 atacagcgag ctgccggaag agattaccga cagcccgatg attgatcgta atgagagttt
      781 tacctttgtc accggcgtga gctggcgttt ttaatttacc ggtggatgtc ggtgcggctg
      841 gcctacgctc tcgcaccgac tcgtgggcct gataagacac gttaagcgtc gcatcaggca
      901 atgtgctccc tacgttggat gcggcgcgag cgccttatcc aacctccgga tggcaatgct
      961 tttcctcatt cacccagatc cgcatttcgc cttcaccccg gttggcccag ctaaaccacg
     1021 ggataaaggt cagggtttgc ggctggcgtt tggctggcgc gctgtcgtaa tgccacagcg
     1081 gttgctgctc tggattgctc tgttcatacc gataacccgg tgcctggatt aagatcttat
     1141 ggcggaacaa tcccttacct tcaaatgtcg taaatggcgc atcggcgggc agccacagat
     1201 tatgcagtga ctcgccgttg tcggcctgtt ccaggcaata caccagcggg ccgcgctgaa
     1261 tcgccacttt tccggcgacg tgacgcacca gagggttacc gtaaacgcga cgtaccggca
     1321 ttggcaaagt cagattcagc gtgtcgccct cctgccattc gcgggtaatg tgcaaatacc
     1381 ctttacgaat atcctgctcg acctcttccc cattcaatat gatctgcggc tgtgtgcacc
     1441 agtccggcag acgtaaagcc agcgtatgac gcaccggctg gggcgattca accgcaattg
     1501 tcacctgttc ctgccacgga tagtttccgc taacccgcag acgcagcgtg ccattttcta
     1561 ccggcacttc catgctgttt cctgcgtaga tgttgatata caacgcatct tcacgcggcg
     1621 tgtagagata atgaccaatc gaggttagca cgcgggcgat atttggcgga caacaagcgc
     1681 aaccaaacca acgctggcgg atcggcttaa cgtgatcgta gatatggttg aattttaacg
     1741 tttttggatg cacttccagc ggattaacgt agaagaaatg tttgccatcc agcgccatgc
     1801 cgccgagcac agtgttgtac agtgcgcgct ccatcacatc ggcatattga ctgtcgcctt
     1861 ccatttccag cattcgccgg gcgaacatca tcaggccgat ggaagcacaa ctttcggcgt
     1921 aaaccgtgtc attcggcaga tcgtaatcgc tgctgaacgc ttcgccgctg ctttgtgagc
     1981 caatgccgcc ggtaatatat aactgacgct gggccatatt gttccacagc cgcaggcaat
     2041 cctgacgctt gctttcatcg tgacttaaac gcgcgagatg tgcgacgcca gtcatcagat
     2101 atacaaagcg tactgtatgt ccgatggcgg tttgttgctg tgcgatgggc aaatgcgcct
     2161 ggctgtaggc tttatctttc accatccacg ccgggccgta ggtgtgccag tgcgaggtct
     2221 gcccgcgctt ttcatattcc tggtcgtaat agtgcggttg cgcaccacgc tgttcgacaa
     2281 aatagttcgt cagcgccagg tagcgcggct cttcggtcac ttcatacagg cgcattagtg
     2341 ccagttcaat ttccgggtga ccaggataac cgtgtaactt actttcacct ggaccaaata
     2401 cgctgtcgat atgatcggcc agacggcaaa ccacttccag caagcgccgt tttcccgtgg
     2461 cctggaagaa ggcgactccg gcttcaatca gatgaccggc gcagtaaagt tcatgacact
     2521 ccgccagatt gctccagcgt tcttcgggcg cttttaccgt aaagtaagca ttgagatagc
     2581 catcttcgca ctgggcggag gcgatcagtt caatcacctc gtcagcggtt ttttccagtt
     2641 cggcatccgg cttctggcac agcgaccagg ctaccgcttc cagccatttg gcgacgtcgc
     2701 tgtcctgaaa caccatcccg taaaattcac cgtcttgcag tcctgcggca atgcgaaagt
     2761 tttcaatcgc atggctgggt tccgcttctg ggatacgatc gttcaaggcg tcccactgat
     2821 aaggaatcac cacatcgcgg accagttgtt ggtactgacc gaggaacgga tcgctgaccg
     2881 tcagtttatg cagatcgact tccgaaatgt tcatcttgtg ctccttaagg ctgtacatga
     2941 cgctgacgca ggtcgctagc aatgcgtgac atcatagggt tattgagttt gcaaaagcgg
     3001 atcgcccctg ccagcagcag gtggaatagc gcaggcagta atgtttccat cgccgtgatc
     3061 ccgccaagcg aactggtggt ctggttttcc acgcctggct ggtaggcgac aaaaataaac
     3121 agcaggctga tgatcccggc gctggaggcc catgccagct tgatgaaaaa cagattgaag
     3181 gcgaagttca tgccggaaga acgtacgcgg gttttccact cgccgtagtc atcggcaaag
     3241 gccatcaatg agaagtgcag tggcaatgtg aagccaagaa tcacgccatt accgaggatc
     3301 accgccagcc acagcgtttg ataagccggg ccagaaggca ggaaccacat caacaccgcc
     3361 agcgcagcga ggagcaggtt ggtgtagtag taaattttga cggtatcgaa acgccgcgtt
     3421 aacgggctga caatcaccga gccgataatg gaggcgaagg tgaccatggt gaagaacagc
     3481 gacgtgtagc ccgtactgcc ttgtaagacg taggtaatga agtacatata cccaccgccg
     3541 cgaatattaa agacgttaat cagcaggaaa gacatgacca gcatcagcag cagttggtcg
     3601 ttgttccgca gcccggcaag atgttcgcgc agggtaaatt tccccattgt ggagagtggc
     3661 acccgttcac gaacccagaa gaaacagcac aggaacatca ccacggcaat ggcgcacagc
     3721 acgccgacgc ccagttgata gccgcgcgcg gcattaccct gaccgaagag atccaccatc
     3781 cacggcaggc caaccgaaac cagaaatccc gctacgccgc agagaacgaa tcgccaggac
     3841 tggcaggaga tcacttcatt gtggcgggtg gtcatggtgt tgatcagcgc gcagtacggt
     3901 acgttgatgg cggtataacc gactgaaagg agcaggtaag taccgaacgc ccaggcgatt
     3961 tttactccca tgctggcatc tggcacggtg aacgtcagga tgccgataat gccgatggga
     4021 atggcaatcc ataactgcca cggacgaaag cgcccccagc gactttgcgt ccggtcggca
     4081 atgacgccca tcaccgggtc ggagatggca tcaaacacgc gcagtgcaat aaacagcgtg
     4141 ccgaccagcg ccggagttaa gccaaaaacg tcggtgtaga aaaaggtgag aaagttcatg
     4201 atcaggcaag ttattacggt gccgcctgcg tcacccaggc cgtagcctat tttttcccgt
     4261 acagataacg catcgtcaat tttaccttta gcgatatcag cggcagtaat cggtgtagag
     4321 gtcatagata ttcctcgtga atagggagca atttttagtt ttattgcaat gacctgatta
     4381 attctgccgt gaacgggaat atcaacaagg cgagaaaaga gtaaaaaaag gtgacgattc
     4441 cgacctgaat ttaaaagtgt gaaatggatc aggtgaaatt aatgtttttg attaataatc
     4501 aaaaggtaat gtgtttaatc attaatgaat aagcgggaaa ttacatatca atgctcgaat
     4561 tatccatgac gttaccgatt aaagtgcaaa atggtggatt atttatttcc cgtggcgtag
     4621 ggaggcaccc ggcaaggcgg ctggagtcat gggaaattat ctttgtcgaa aaaggtcgat
     4681 taacgattca ggaagaagag cggatatttc tggtcgatgc gggagagagt ttattactct
     4741 ggccgaaccg acagcatgtt ggggtagagg attttcccgc cgatctcaaa ttttactggc
     4801 tgcattttga ggttaaagcg ccggatagcg acccgcgctg gctcactcat cttagcgtgc
     4861 cgcaacaaac gaaggttgcc gatccgcagg cgctggtggc cctgtttcgc cagttcttaa
     4921 gcgaacagga aaagcatcaa cgcactccgg cgctggagtt tattgtgctg ctgattttgc
     4981 agcagctcac ccgtgacgcg cgtcttgatg aacaggccga tgccgcaggt accacgcttg
     5041 cctggaaagc gcagaaactg attcgcacgc aatatcatcg cccgctttct acggcgcttt
     5101 tagcccgcga gctgtactgc aatgccgatt atcttgggcg cgtctaccgg cgggtgtttc
     5161 atctgacgtt aacagaagca attcatcgcc agcgagtgcg cgaagccgaa aagctgttaa
     5221 ttagcgaagc gcgatcgctc actgaagtgg ctgcgttgtg cgggtttaat gacgttggct
     5281 atttccgaca gatttttcgc aaacacaccg gtttaacgcc agctgcgtgg aagcgacgtt
     5341 acagcaagga acatatcaat tcgtagtgcc ggggcaatga agccccggcg tgagggatta
     5401 ctgcccgtaa taggcattgg ccccatgctt acgcaggtag tgcttatcta acagttcgtt
     5461 ttgcatcgca gggagctgcg gcgcaagctg gcgcgagaac aggcccatat aggcgcactc
     5521 ttccagcact acggcattat gcaccgcatc ggcggcattt tttccccagg cgaacgggcc
     5581 gtgagaatgc accagcaccg ccgggatttg tgccggactc ctgccgcgtt cttcgaaagt
     5641 ttcaatgatc acttcgccgg tctgatattc atattcgccg ttaatctcct ctgcggtcat
     5701 ctgtcgcgtg caggggatgg caccatagaa ataatcggcg tgcgtggtgc cccaggcggg
     5761 gagatccagc ccggcctgcg accagatggt ggcgtggcgc gagtgggtat gcacaatgcc
     5821 accgatttcg gcatagcgac ggtaaagcgc cagatgcgtt ggcgtgtcgg acgagggctt
     5881 tttgctgcct tccaccacct taccgctggc tatctcaacc accaccatat cgtcggcggt
     5941 catcacgtcg tactcgacgc cggaaggttt gattaccatc cattgccgcg cttcgtctac
     6001 cgcgctgaca ttgccccagg tgaacgtcac cagatggtga gcgggaagtg ccagatttgc
     6061 cgccagcacg tcggctttca gttgctctaa catataaaac ctgcctcctg catacgcgct
     6121 tcaatccagc gccgcgcctg aataatctcc agcaccggct ctttggcttt ttcggtccac
     6181 atctcaatca ggaaagaacc gcgatagttc agttcatgca gcgttttgaa gatgccaacg
     6241 aaatcgacgc agccttcgcc aaacggcaca tcacggaact gtccggggct ttgctcggtg
     6301 actggttgag tatctttcag gtggatcgcg gcgatacggt caatgcccag tttcagttcg
     6361 gcgggaacat cattgcccca ggcgctgagg ttgccgacgt ccgggtagac ggtgaaccac
     6421 ggcgaggcga gcatctcgtc ccactttttc catttgctga tggagttcat aaacgcagta
     6481 tccataatct ccaccgccag cattacttgt gatgccgccg cctgttcgac tgtccacgcc
     6541 agcccttcag caaaacgttg ccgggtgcct tcgtcgtggt cttcgtaata gacgtcgtag
     6601 cccgccagct ggatggtgcg aatgccgaga tcgcgcgcca ggcgaatcgc tttactcatg
     6661 atttcccgcg cccgttcgcg cactgcctcg tcacggctac caaaaggaaa gcgacgatgt
     6721 gcggacaggc acatcgacgg gatcccaaca cccgtttcga tcatcgcagt gaccagcgaa
     6781 gcacgttgtg cggcgctcca gtcaagacgt gagagccgtt cgtcggtttc atccaccgac
     6841 atttcgacaa aatcaaagcc acagcttttt gccagcacca gccgctccgg ccaggagaga
     6901 tctttcgcca gcgctttttc ataaatccct aacggatgat tacgcacgcg cacctcccca
     6961 gatggcatca atttgtgcat ggaaatcgcc agccacctgc accggatttg ctgcgcctgc
     7021 cagtgctcgc ccggcaataa acgctttcac gcgaatatct ttaaacagcg gcaggtcagc
     7081 aggggtaatc ccgccggtaa tggaaagctc aaggccgata tcggaaagcg ccttcatgcg
     7141 tgccagatcg gcctcgcccc actgttgccc gctggcctgc gcgtcgcgac cgcgatgata
     7201 aatggcctgc cgcacgccaa tacgatgcca gtcgcgggcg tcgtccagcg tccagttacc
     7261 gaacaactcg atctgaattt caccaccgca gcgttgcgcc atcgcgtggc ctttttccac
     7321 cgtcgcgagc ggtgcggcgc agatgatggt catccagtta gcgcctgcgc caaacgcctg
     7381 ttgcgcgagc gtttcaccgg catcggcgac cttccagtca gcgacgatga ttttgttcgg
     7441 gcactgttcg cgcaaggctt tcactgcgcc aagcccttcg tttaaacaga gaatggtgcc
     7501 cgcttcgacg atatcgacgc tgtcttttaa cagcgtcacg tcgcgctgtg cgtcttcaag
     7561 tgatgagtgg tcgagggcca gttgcagaag tggtcggctc ataatgtgtg ctccttgatg
     7621 cgggcgtgaa agccctgaag tgcggcaatg agatgctgat aacgttggta tttttgttgg
     7681 taaagctgat gggcggtcat atccggcagc agggtgcgta ccgggtgctg taaatcacgt
     7741 tgggcttcgc tgaaatcgcg ataaactccg gtgccgacgc gggcggcaag tgccgcacca
     7801 aagcagccgg tttcttccac ctgcggcagc tcgatacgca gaccgctgac gtccgccagc
     7861 atttgcatcc agacatcgga gtgcgccgga ccgccagtga cgcgcagggt gtgcacatca
     7921 gtaaaacgtt cgcgcatccg gttgagatgg gtcatatggc tgaacaccac gccttcataa
     7981 atggcctgca acaggtgtac gcgggtgtga atggcctgca tcccgtagaa accgctggtc
     8041 atctcgagtc cagcgttgct gccgtacagg aacggcagga aaaagagatc gcccccggcc
     8101 ttcggcaagc tggcaacggc ctggttgatc tcatcaaacg agatttctcc ccactgtgtg
     8161 gtaaaccatt cgagattgcc ggaagaggtg gggcttgctt catgaacgat aaattgacca
     8221 tcgttaacgt agcgaccata gacatacgga tgcgcttcac cgtcacgtaa accacgggtt
     8281 atgccgctgg tcaccgccca ggtgcccatc accgcattga gggtaaattc gtcttcgatc
     8341 ccggcgcaga gtgcggtgga aaccacatca aacaggccgc cgacaacggg cgtacccgct
     8401 ttcagaccgg tcagtgcggc tgtctgagcg gtgatctccc cgcagatttc ggcagatccg
     8461 acaacaagtg gcagggcgtg gttgatttca gcgatcccca gccagtcggt gaggcacggg
     8521 tcatattccc caagactcat gttgtagaga ttggactcag aaatattgct ctcttcgcaa
     8581 cctttgacgc cggttaaaca ccagcgcagg tagtcgtgcg tcatcatcac gcagccaatt
     8641 tgcgcgtagc gttccggttc gtgctctttc agccagcgta acagcgacac cggatgcccg
     8701 gtccacaagg tttgtcgcgt cagcgggtag agtttttccg ggatgccatc ttcctgccag
     8761 cgacgaacga tttccatcgc ccggcgatcc gaggacaaaa tggcgttacc gagcggcttg
     8821 tcgtttttat ccagcaaaaa caagcccttt ccctgtgcgg agatgccgat accgacaatt
     8881 tgttccccgc taacaccaga atgagtaagc agggcgcgaa tgacagccat gcagcattgc
     8941 cacagttctg ccatatcgcg ctctgcccaa cctggctgcg ggcttaatgc gcacagcggc
     9001 aggcgctgca cgcctgcctc ccggccttcg cggtcataca gcccggcttt cagccagcta
     9061 ccgccacaat ctaaccccag ccagtattgc gtcatggttt attgcacctc atccacggct
     9121 ttcagcagct cagcgccctg cggaacatct ttaacaaaca tgtcgcggac ctgagtaccc
     9181 agtgcgtcgc taaaggcttt gcggtcgaga tcggtgatga cttccacgcc cgcttctttc
     9241 atgccgtcga tgattttttg ctgatcttcg gcaaccagtt tgcgctggta gttacccgct
     9301 tcctgtgcag atgaaactag cgcctgctgg aactccgggc ttaagccatc aaacttcgct
     9361 ttgttgatca ccaccagaag tggcgaatag gcgtggtgcg tcagagaaag gtacttctgt
     9421 acttcgaaaa attttgctga ccagacgacg ttgatcgggt gttcctgagc gtcgatagtg
     9481 cgcgtttcca gcccggtata gacttcggca aacggcatcg ggatcgggtt agcgccaaag
     9541 actttgaatg cggcgatatt catcgggcta ttgttggtgc gaattttcag ccctttcagg
     9601 tcggcggggg ttttaaccgg tgcgcgcgag ttggtgacat cgcgccagcc gttttcccag
     9661 taggccagta cttttagtcc tttaccttca agtgaggctt tcagatcatc accgactttg
     9721 ccgtcgagcg ttttatgcgc gtgagcggta tcgcggaaca ggaaagggac atcaagcaag
     9781 ttcatcactg gtgataaccc ggcaaagtta ttcgagccgg acatttccat atcgatggtg
     9841 ccgccacgta cgccgctgat catcgcctgt gcgttaccga gagtactgtc cgggaacagt
     9901 tttaatttca gctcgccttt ggttctctcc tgcaataaat cattgaattt tttcgccgca
     9961 atatgttgcg agtcggtttg tgaggtttca taaccgaaac gtaaagattg cgccgccagt
    10021 gaagatgtac taaatgcagc caggccagca atgaataatg cgtaggttac agagcgtaat
    10081 ttcataatat tttcctttta attaatccat ttcaaaggga ggatgatgag atcgggaata
    10141 aaaacaaata cgactaataa tgaatataaa accaggacgt aagggaaaac gcctctgact
    10201 gcatcatcga atttgagttt tgccacccct gaaataacgt taagcacatt accgataggc
    10261 ggtgtaatta agccgattga gcagttaatg ataaacatga caccgaaata aatcggatcg
    10321 attcctgcct ctttaactaa aggcattaat accggggtaa gaattaatac cgtcggcgtt
    10381 aaatccatga ccatgccgac aatcaggata gccaccataa tgacgataaa cagcagacgc
    10441 ggtgaatcga ccagcggttg cagtaaatca gaaaccatca tcggcagttc agcaatggtg
    10501 atcagccagg cggaaacttg tgccgaggct accagaaaca tcaccactga ggtcgttttc
    10561 gctgcgccaa tgagcacatg ccaaagtgtg gcgaaggtca tttcacggta aataactgtg
    10621 gcgacaaaca gcgcatagaa agcagcaacg gccccggctt cggtaggggt aaacagaccg
    10681 gagcgaaacc cgccaataat gatgactgga agaaacagcg cccagatacc ggagacaaaa
    10741 gagtgccaga tttcctgcat cgttgctttt tgctggcggg gtaaattaag gcgactggcc
    10801 tgccaccacc aggtaagcat cagcgttgcg cccatcatca tgccaggggc aatgcccgcc
    10861 ataaacagct tactgatgga taatccgctg gaaacgccga aaataataaa cggaatggaa
    10921 gggggaataa ttggcgcgat aatgccgcca gaggcaatca gccccgcagc ccggttgacc
    10981 gggtagttag cgctgcgcat catcggcact aatagcgcgg caacggcggc ggtatcagca
    11041 acagcagaac cagaaaggct cgccataatc atagccgcca gcacgccgac gtagcccaac
    11101 ccgccaggtt tatgccccac cagtttcatc ggcaggtcaa caatgcgttt tgacaagccg
    11161 cccgcattca tgatttcacc cgccagcaca aagaacggaa tcgccagcag ggagaagctg
    11221 tcggctccgt tcaccagcgt ttgtgccatg atctggacat caaacatgtc cagccagaac
    11281 attaacgccg ctccgcacaa caacagtgcc caggcaatag gcaaaccgat agcaatacca
    11341 cccaacagac agcccagaaa aatcagcaca gccatgatta agctccttgc ggtggcgacg
    11401 ttagagaatt gctacgcgtg atgagttgat ataaatgacg cagttcaaag aatgcgataa
    11461 cgaggctggt gggcagacaa gcggcataca tcaggccgat gggcaaaccg aggatcggtg
    11521 aataatcgct ccagtcctga attgttttta gcgttgcgcc ccaggccagt gcgccacaaa
    11581 taaataagat taaggaatgg gtaaccagag cgactcgtcg ctgccatgcg ggggagagtt
    11641 tctccaccag aaaggtgacc tgaacgtggg cgttatccat aaaagccaca atcgcgccaa
    11701 taaatgttaa ccagacaaat aaataacgtg acaattcatc tacagataaa atgcttgtct
    11761 gaaaaccata tcttaaaata at
//
LOCUS       DAZKJR010000152        11618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKJR010000152 DAZKJR010000000
VERSION     DAZKJR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.153"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            261..2684
                     /gene="pgaA"
                     /locus_tag="ACHOWS_004279"
     CDS             261..2684
                     /gene="pgaA"
                     /locus_tag="ACHOWS_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFQ0049769.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLVELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2693..4711
                     /gene="pgaB"
                     /locus_tag="ACHOWS_004280"
     CDS             2693..4711
                     /gene="pgaB"
                     /locus_tag="ACHOWS_004280"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFQ0049770.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4704..6029
                     /gene="pgaC"
                     /locus_tag="ACHOWS_004281"
     CDS             4704..6029
                     /gene="pgaC"
                     /locus_tag="ACHOWS_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFQ0049771.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6031..6444
                     /gene="pgaD"
                     /locus_tag="ACHOWS_004282"
     CDS             6031..6444
                     /gene="pgaD"
                     /locus_tag="ACHOWS_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFQ0049772.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(6494..7282)
                     /gene="phoH"
                     /locus_tag="ACHOWS_004283"
     CDS             complement(6494..7282)
                     /gene="phoH"
                     /locus_tag="ACHOWS_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFQ0049773.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7908..9179)
                     /gene="efeB"
                     /locus_tag="ACHOWS_004284"
     CDS             complement(7908..9179)
                     /gene="efeB"
                     /locus_tag="ACHOWS_004284"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFQ0049774.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGVISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9185..10312)
                     /gene="efeO"
                     /locus_tag="ACHOWS_004285"
     CDS             complement(9185..10312)
                     /gene="efeO"
                     /locus_tag="ACHOWS_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFQ0049775.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKVNPELLAKVDANFIKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10370..11200)
                     /gene="efeU"
                     /locus_tag="ACHOWS_004286"
     CDS             complement(10370..11200)
                     /gene="efeU"
                     /locus_tag="ACHOWS_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFQ0049776.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSVFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3075 a         2852 c         2723 g         2968 t
ORIGIN      
        1 attttaatcc taatttattt tgaaaaaggc attgggattt atgccgtatt cctgaagatc
       61 ctcatcattg gaatggattt tcgggcgaga aaaggatttt atatggacaa tctgctcatc
      121 atttcttctt ctcatcatca acaattcacg tgtctcttcc gcgtttaata acggattatg
      181 agatgcaaaa atatctttct tttcagttac ctgtaattag atatagagag agattttggc
      241 aatacatgga gtaatacagg atgtattcaa gtagcagaaa aaggtgcccg aaaaccaaat
      301 gggctttgaa acttcttact gccgcatttt tagcagcgag tcccgcggcg aagagtgctg
      361 ttaataacgc ctatgatgca ttgattattg aagctcgcaa aggaaatact cagccagctt
      421 tgtcatggtt tgcacaaaaa tcaacactca gtaataacca aattgctgac tggttacaaa
      481 ttgccttatg ggccgggcaa gataaacagg tcattaccgt ttacagccgc taccgtcatc
      541 agcaattacc agcgcgtggt tatgcagctg tcgccgtcgc ttatcgtaac ctgcaacaat
      601 ggcaaaactc gcttacactg tggcaaaagg cgctctctct ggagccgcaa aataaggatt
      661 atcaacgggg acaaatttta accctggcag atgcgggtca ctatgatact gcgctggtta
      721 aacttaagca gcttaaatct ggagcgccgg acaaagccaa tttactcgca gaagcctata
      781 tctataaact gacggggcgt catcaggatg aattacgggc gatgacagag tcattacctg
      841 aaaatgcatc taagcaacaa tatcccacag aatacgtgca ggcattacgt aataatcaac
      901 tagctgccgc gattgacgat gccaatttaa cgccagatat tcgcgctgat attcatgccg
      961 aactgatcag actgtcgttt atgcctacgc gcagtgaaag tgaacgttat gccattgccg
     1021 atcgcgccct cgcccaatac gctgcattag aaattctgtg gcacgataac tcagaccgca
     1081 ctgcccagta ccagcgtatt caggttgatc atcttggcgc gttattaact cgcgatcgtt
     1141 ataaagacgt tatttctcac tatcagcgtt taaaaaatac gaggcaaatt attccgccct
     1201 gggcgcaata ttgggttgcg tcggcttatc tcaaagaaca gcagccaaaa aaagcacagt
     1261 caataatgac agagctcttt tatcacaagg agaccattgc cccggattta tccgatgaag
     1321 aacttgcgga tctcttttac agccacctgg agagtgaaaa ttatcccggc gcgctaactg
     1381 tcacccaaca taccattaat acttcgccgc ctttccttcg gttaatgggc acgcctacga
     1441 gcatcccgaa tgatacctgg ttacaggggc attcgtttct ctcaaccgta gcaaaatata
     1501 gtaatgatct tcctcaggct gaaatgatag ccagagagct tgcttataac gcaccaggaa
     1561 atcagggact gcgcattgat tacgcgagtg tgttacaagc ccgcggttgg cctcgtgcag
     1621 cagaaaatga attaaaaaaa gcagaagtga tcgagccacg taatattaat ctggaggttg
     1681 aacaagcatg gacagcatta acgttacaag aatggcagca ggcagctgtc ttaacgcacg
     1741 atgttgtcga acgtgaaccg caagatcccg gcgttgtacg tttaaaacgt gcggttgatg
     1801 tacataatct tgtagagctt cgtatagctg gttcaacagg aattgatgcc gaaggcccgg
     1861 atagtggtaa acatgatgtc gacttaacca ccatcgttta ttcaccaccg ctgaaggata
     1921 actggcgcgg ttttgctgga ttcggttatg ccgatggaca atttagcgaa ggaaaaggga
     1981 ttgttcgcga ctggctggcg ggtgttgagt ggcggtcacg taatatctgg ctcgaggcgg
     2041 agtacgctga acgcgttttc aatcatgagc ataaacccgg cgcgcgcctg tctggctggt
     2101 atgattttaa tgataactgg cgtattggtt cgcaactgga acgcctctct caccgcgttc
     2161 cattacgggc aatgaaaaat ggtgttacag gcaacagtgc tcaggcttat gttcgctggt
     2221 atcaaaatga gcggcgtaag tacggtgtct cctgggcttt cactgatttt tccgacagta
     2281 accagcgtca ggaagtctca cttgagggtc aggaacgcat ctggtcttca ccatatttga
     2341 ttgtcgattt cctacccaat ctgtattacg aacaaaatac agaacacgac actccatact
     2401 acaaccctat aaaaacgttc gatattgttc cggcatttga ggcaagtcat ttgttatggc
     2461 gaagctatga aaatagctgg gagcaaatct tcagcgcagg tgttggtgcc tcctggcaaa
     2521 aacattatgg cacggatgtc gtcacccaac tcggctacgg gcaacgtatt agctggaatg
     2581 acgtgattga tgctggcgca acgctacgct gggaaaaacg accttatgac ggtgacagag
     2641 aacacaactt atacgttgaa ttcgatatga cattcagatt ttaaggataa atatgttacg
     2701 taatggaaat aaatatatcc tgatgctggt gagtataatt atgctcactg cgtgcattag
     2761 ccagtcaaga acatcattta taccgccaca ggatcgcgaa tctttactcg ccgagcaacc
     2821 gtggccgcat aatggttttg tggcgatttc atggcataac gttgaagacg aagctgccga
     2881 ccagcgtttt atgtcagtgc ggacatcagc actgcgtgaa caatttgcct ggctgcgcga
     2941 gaacggttat caaccggtca gtattgctca aattcgtgaa gcacatcgag gaggaaaacc
     3001 gctaccggaa aaagcagtag tgctgacttt tgatgacggc taccagagtt tttatacccg
     3061 cgtcttccca attcttcagg ccttccagtg gcctgctgta tgggcccccg tcggcagttg
     3121 ggtcgatacg ccagcggatg aacaagtaaa atttggcgat gagatggtca gtcgagaata
     3181 ttttgccacg tggcaacaag tgcgagaagt tgcgcgttcc cggctcgttg aggtcgcttc
     3241 tcatacatgg aattctcact acggtattca ggctaatgcc accggcagct tattgcctgt
     3301 atatgtaaat cgtgcatatt ttactgacca cgcacggtat gaaaccgcag cagaataccg
     3361 ggaaagaatt cgtctggatg ctgtaaaaat gacggaatac ctgcgtacaa aggctgaggt
     3421 aaatccacac gtttttgttt ggccttatgg cgaagcgaat ggcatagcaa tcgcggaatt
     3481 aaaaaaactc ggttatgaca tgttcttcac ccttgaatca ggtttggcaa atgcgtcgca
     3541 attagattcc attccgcgag tattaatcgc caataatccc tcattaaaag agtttgccca
     3601 gcaaattatt accgtacagg aaaaaccact acaacgggta atgcatatcg atcttgatta
     3661 catttatgac gaaaaccgcc agcaaatgga tcgcaatatt gatgtgctaa ttcagagggt
     3721 aaaagatatg caaatatcta ccgtgtattt acaagcattt gctgatcccg atggtgatgg
     3781 gctggttaaa gaggtctggt ttccaaatcg tttgctacca atgaaagcag atatttttag
     3841 tcgggttgcc tggcaattgc gtacccgctc aggtgtaaac atctatgcgt ggatgccggt
     3901 attaagctgg gatttagatc ccacattaac gcgagtaaaa tacttaccaa ctggggagaa
     3961 aaaagcacaa attcatcctg aacaataccg ccgtctctcc cctttcgatg acagggtcag
     4021 agcacaagtt ggcatgttat atgatgatct tgccggacat gctgcttttg atggcatatt
     4081 gttccacgat gatgctttgc tttcagatta tgaagatgcc agtgcagcgg ctatcgcggc
     4141 ttatcagcaa gctggcttta gcgggagtct gagcgaaatt cgacaaaacc cggagcaatt
     4201 caaacagtgg acccgcttta aaagtcgtgc gttaactgac ttcactttag aacttagtgc
     4261 gcgcgtaaaa gccattcgtg gtccacatgt taaaactgca cgaaatattt ttgcacttcc
     4321 ggtaatacaa cctgaaagtg aagcttggtt tgcacagaat tatgctgatt tcctaaaaag
     4381 ctatgactgg accgctatta tggctatgcc ttatatggaa ggcgtcacag aaaaatcggc
     4441 tgatcaatgg ttaacacaat tgaccaatca gatgaaaagc atcccgcagg ctaaagacaa
     4501 atcaatttta gaattacagg cacaaaactg gcagaaaaat ggtcagcatc aggctatttc
     4561 ttcacaacaa ctcgctcact ggatgagcct attacaactg aatggagtga aaaattatgg
     4621 ttattatccc gacaattttc tgcataacca acctgaaata gacgttattc gtcctgagtt
     4681 ttcaacagcc tggtatccga aaaatgatta atcgcatcgt atcgtttttt atattatgtc
     4741 tggtgttatg catacctcta tgcgtagcgt actttcactc tggtgaactg atgatgaggt
     4801 tcgttttctt ctggccgttt tttatgtcca ttatgtggat tattggcggc gtctatttct
     4861 gggtctatcg tgaacgccac tggccgtggg gagaaaacgc accagccccc cagttgaaag
     4921 ataatccatc tatctccatt atcattccct gttttaatga ggagaaaaac gttgaggaaa
     4981 ccatacatgc cgctttagca cagcgttatg agaacattga agttattgcc gtaaatgatg
     5041 gttcaacaga caaaacccgt gccatcctgg atcgcatggc tgcacaaatt ccccgtctgc
     5101 gggtcattca tctggcgcaa aaccagggga aagccattgc gcttaaaacc ggagctgccg
     5161 cggcgaaaag tgaatatctg gtgtgcattg atggcgacgc gttattagac cgcgatgcag
     5221 cggcatatat tgtggaaccg atgttgtaca acccacgtgt gggtgccgta accggtaatc
     5281 ctcgtattcg cacacgttct accctagtgg gtaaaattca ggttggcgag tattcctcaa
     5341 ttattggttt gatcaagcgg acccagcgta tctatggaaa tgtatttacc gtttccggtg
     5401 ttattgccgc atttcgtcgc agcgccctgg cagaagtggg ttactggagt gacgatatga
     5461 tcaccgaaga tattgatatt agctggaagc tgcagttgaa tcagtggacg attttttacg
     5521 agccacgggc gctgtgctgg atattaatgc ctgaaacgtt aaaagggctg tggaaacagc
     5581 gcctgcgctg ggctcagggc ggtgcagaag tattcctcaa aaatatgaca aggttgtggc
     5641 gaaaagaaaa ctttcgaatg tggccactgt tttttgaata ctgcctgacg acaatatggg
     5701 cctttacctg cctggtcggt ttcattattt acgcagtcca acttgcaggt gtaccgttaa
     5761 atattgaatt gacacatatc gcagcgacac atactgccgg aatattattg tgcacgttat
     5821 gtttactgca atttattgtc agtctggtaa ttgagaatcg ctatgagcac aatctggctt
     5881 catcgctttt ctggattatt tggtttccgg ttattttctg gatgctaagc ctggcaacga
     5941 cattggtatc atttacacga gtcatgttga tgcctaaaaa acaacgcgct cgttgggtaa
     6001 gtcccgatcg tgggattctg agaggttaat atgaacaatc taattattac gacccgacaa
     6061 tcgccagtac gtttactggt tgattatgtt gccacaacca tcttgtggac attatttgct
     6121 ttgttcatat tcttattcgc cattgatctg ctgacgggtt attactggca aagcgaggcc
     6181 agaagccgac ttcagttcta ttttttgctg gcagtggcga atgccgtcgt gttaatcgtc
     6241 tgggcattgt acaataagct gcgttttaaa aaacagcagc atcatgcagc ataccaatat
     6301 acgccgcaag aatatgcaga gagcttagca atacctgatg agctttatca gcaactacaa
     6361 aaaagccaca ggatgagcgt acacttcacc agtcagggac aaataaaaat ggtggtttca
     6421 gaaaaaacgc tagtccgggt ataaacaccc aaaacaaagc ccggttcgcc cgggctctgc
     6481 accgataata cacttaactg tatgcatgta gcgtacgttg gcaaagtgcc gaacgtacgc
     6541 aatcctcttt accgaatcgg atgatcccga ccatttcatc ttcttcgaaa cgttccagcg
     6601 cgtcacttaa tccggagcgc acaccgcgag gtaaatcgca ttgcgtgata tcaccgttga
     6661 cgataaccgt cacgttctcc ccgaggcggg ttaaaaacat tttcatttgc gcggcagtca
     6721 cattctgcgc ctcgtcaaga atgacgactg cattttcaaa ggtacgtcca cgcatatagg
     6781 cgaacggcgc gatttcgacc ttccctattt ctggtcgcag gcagtactgc ataaaggaag
     6841 cccctaagcg ccggaccagc acgtcgtaaa ccgggcgaaa atagggagca aacttttctg
     6901 cgatatctcc aggtaaaaag ccaagatctt catcggcttg cagaactgga cgggtgacga
     6961 taatcctgtc gacatcctta tgtatcaggg cctctgccgc ttttgctgcg ctgatccagg
     7021 tttttccgca cccggcttcg cccgtggcga atatcagctg cttactctca atagccttca
     7081 gatagtgcaa ttgcgcttca tttcgcgcga ggatgggcga ggtatcgcga ctgtcgcggg
     7141 ccataccaat ggcttctacg ccgctcattt gcacaagcga ggtgaccgat tcttcttcac
     7201 gctgcttatg gctgcgcgaa tcccgtctca gcacacgttt tgcctcgcga cgagctttga
     7261 tcactgcttt ttgtcttccc atggagagca ccttgagttg tttgtattca tcacacgcgc
     7321 cgttggcagc gcgattatgc gcacgaacat cagagggttg gcttccttgt aagccatagt
     7381 ttgcttttgg ataaaatgcc gaaaaacggc tacgcacacc gtttgcggcg tcggtaacac
     7441 gagtaaagag aggaagtgag aaaaatacag agtgacgaag ttgctaccgg aggagaaatc
     7501 tccgcgagtg gtggagcctt gcttaacaaa acattgtcca gaacaggacg ttaccatccg
     7561 cgatctccat agtgactgac tatcactgcc gggaacttcc gctgctactt aataagtaca
     7621 acagatctcg cattcattgc aacaatatat tcacttatat ttaaccataa aacaccattt
     7681 cagtgacatt agtttctact ggaaagatga caaagtgatg acagtgataa aaaagctgtg
     7741 tgctttcagc aggattcgca gtactggtaa aagcttgccg gatgcggcgt gaacgcctta
     7801 tccggcctac cgaacccgca ccgattgtag gcatgataag acgcgcaagc gtcgcatcag
     7861 cgcacgaacg cctggtgcgg cgtaaacgcc ttatccgcct aaaaacatta aacccgcaat
     7921 aatgcgcttc cgagataatc gttcgcgtcc ttcacacccg gcagcgcaaa gaaatagccg
     7981 ccaccgatag gtttgacata ttcctccagc gcctctccat tcaacctttt ttgtactgtc
     8041 aggaagccct tttccagatc gtgttggtag cagacaaaca gtagccccat atcgagttgc
     8101 ccggaattgg tgacgcccaa cgaataactg tagccacgac gcagcatcag acttgactca
     8161 ctctctggcg tgcggggatt cgccagccgg atatggctgt ccagcgcgat caccttcccc
     8221 tccggatcgc tggcgtaatc aggcacatca tgctcatgct gcattcccag cggcgcgccg
     8281 gtttgcttat cacgcccaaa tatcgtctgc tgttctttca gcggcgttct gtcccaaaat
     8341 tccacccgaa actgaatcaa ccgcaccgcc tggtagctac caccgattgt ccacgcaggc
     8401 tcctgctgat ctgccgtcac ccacaccact ttttgcatca acttcgcatt ctggctatcg
     8461 ggattggcag tgccgtcttt gaaacccagc aaattaatcg gtgtctcttt acctttacta
     8521 cgcgccgcgt ggtcggaaat aaccccttcc cgcttccagc gcacgctgag caaatccggc
     8581 gtgtgtttga tgatatcgcg cagcgcatgg atcaccgtgt cctgggtgtt ggcgcaaatt
     8641 tgtagcaaca catcaccatg acataacgcc gcatccagcg agtcgttggg gaaacgcgtc
     8701 atcttctgca actttttcgg catctgtggc gcaaggccaa agcgctcatc aaacaatgag
     8761 tggcccaccg ataacgttat ggtgagatta tcgggcgcaa tgtagccgcc aagaatgccg
     8821 gaatcgagtg gtggcaggcg cggatttggc gtttctggtg ccgctccgcc ctgagtcaga
     8881 aaagcaaaac gctgagtcaa caagcgaaac aaccgttcaa gatcggcttt atcgctggca
     8941 agcacatcaa acgccaccag catcattgca gcctgttgtg gcgtcaggat ccctgcctga
     9001 tgctcaccat aaaacggctg tttctcattg cgtgcatccg gtgaaagcgt acccggcgca
     9061 ctttgcgttt tttgtgcatg agcgaccgga caacttcccg ccagcgccag cgcaccaatc
     9121 cctttcagta aacgtcggcg tgacggttca ttcacgccgt tttcatcttc atactgcata
     9181 acgcttagtc cagccccagc acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa
     9241 tcggtccttt cagtgcattc cggtcagcat cggtcagttt gtcgtaattt tcaaaaccgt
     9301 ctttagtacg gtatttcgcc agaatggtat cgacctttat gaagttggca tcgacttttg
     9361 ccaacagttc cggattcact ttttgtagtt gtggacgcag cagatcgaca attttctgcg
     9421 agccttcaac gttagcctgg aaatcccaca gatcggtgtg gctgtagcga tcttcttcac
     9481 cgctgatttt gctggccgcc acttcctcaa tcagtccggc tgcaccgccg accacttttg
     9541 aaggagggaa agccagttca ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct
     9601 ggtcggcata cttatccatc cctttggtgg tgttatcgcc aaacaatgct ttttccagac
     9661 ggtggaaacc ggtgaatttc ggatcggcgg ctttttgctc gtaatcatct tcacgggcgt
     9721 caatgctgcc atccagatcg gagaacagtt cagcaatcgg ttcaatgcgc tcatagtgct
     9781 ggcgcgtcgg cgcatacagt gctttcgctt tttcgatatc gcctgcttta atagcatcgg
     9841 taaaggcttt ggtgtcggtc accagctgcg tggtttccgc catgacatac gctttatatg
     9901 cagtaattgc accaccaaga cttaacagcg catcactttg cgccgcatcc gccgttgcct
     9961 cacctttgac gatcaacttc cctttcgggt ttgtcagcag accgcaggtc atatcgtatt
    10021 caccaggctg taaatttgcc gtcattttct ggctaaaacc aggggcgata ttttcccgct
    10081 cttccaccac catcacgcct ttgaggatct cccactccag tgctttctgg ctgtggttct
    10141 gaataatgaa ctgtgttttc ccggcgttaa ccgtaatggt catcggttcg cactgcttat
    10201 ccgtcacggt cactttgacc tgcggcacat cagcggcgtt agccataaaa gcagaagaaa
    10261 acagcgcagc cacgctcaac tgcaatgcgt tacggcggaa gttaatggtc atgacgagac
    10321 tatcccttta aagtatgttg taactaagca agagtttgcg tcgtatttgt tacgcggagc
    10381 gagacgctgt cgcccctgcg cgtggtggca gagcaaatgc caccagcgcc gggatgagat
    10441 aaataaacca gacggcgact tcgctgacgc tcggcgcttc ctgatagcca aaaatccctt
    10501 ccatcagcgt gccaaacacc gagtgagttg agagcaccgc actcatatcg aaggcgattt
    10561 cctgaaagtg gttccacaat ccggcttcat gaaatgcgcg aatggcacca gccgccagcc
    10621 ctgcggcgac gaagagaata aacaggctgg tccatttaaa aaatgcacca agattgaggc
    10681 gaataccgcc ccagtagagc aggaagccga gcaccacggc tgtagcaaga ccgagcattg
    10741 cacccagcgg cggccagatc ccgacatctt gttgaaacgc cgccagcagg aaaaagaccg
    10801 actccagccc ttcccttgca acggcaaaaa agaccatcat caccagcgcc cagccatgat
    10861 gatttccacg ctgcaatgcg ctatcgactg cctgctccag ttgtactttg acgttgcgcg
    10921 acactttgcg catccagaaa accatccagg taaggatcac cacggcgatc accgccacga
    10981 taccttcaaa cagttcctgt tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga
    11041 tacccaagcc caggcacaac gcagcggcaa gcaacacgcc aatccacatc acaccaatcc
    11101 atcggcctcg ctgggtacgc ttaagatagc tggcaatcaa actgacaatc agcgcggctt
    11161 caagtccttc gcgcaacata atgagaaacg gaacaaacat gccacccacc cttaaacgtt
    11221 tttctgagtc aatctatgca aagaaaagta aatcgcagtg atagcgatta tcattacggt
    11281 aaagaaaaat acaagcagaa tgtttataaa atgatgttgg aatgtaaaca aagaaaggta
    11341 atgccagggg atataaacga ttattccagg tacaaagatg ggattgttta caacgagcca
    11401 tgtgttcttg ccagatgccg cgtaaatgcc ttatccagcc gacaaaactg tccagactca
    11461 atgcatcaga gcttccatgt aaaactgata agcgggtgta tcaagcaatt tcgtccatcc
    11521 gcgattatca aatctaactt tttgcccgga acataataac agctcgggtg ttggtggcgt
    11581 caaaatcgct gaagcgttcc ccgaaggccg gggcagcc
//
LOCUS       DAZKJR010000153        11447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKJR010000153 DAZKJR010000000
VERSION     DAZKJR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.154"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(135..941)
                     /gene="trpA"
                     /locus_tag="ACHOWS_004287"
     CDS             complement(135..941)
                     /gene="trpA"
                     /locus_tag="ACHOWS_004287"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFQ0049777.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNLAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(941..2134)
                     /gene="trpB"
                     /locus_tag="ACHOWS_004288"
     CDS             complement(941..2134)
                     /gene="trpB"
                     /locus_tag="ACHOWS_004288"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFQ0049778.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2146..3507)
                     /gene="trpCF"
                     /locus_tag="ACHOWS_004289"
     CDS             complement(2146..3507)
                     /gene="trpCF"
                     /locus_tag="ACHOWS_004289"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFQ0049779.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPAHIATVYRNYASAISVLTDEKYFQGSFDFLS
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3508..5103)
                     /gene="trpD"
                     /locus_tag="ACHOWS_004290"
     CDS             complement(3508..5103)
                     /gene="trpD"
                     /locus_tag="ACHOWS_004290"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFQ0049780.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHVPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDSQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5103..6665)
                     /gene="trpE"
                     /locus_tag="ACHOWS_004291"
     CDS             complement(5103..6665)
                     /gene="trpE"
                     /locus_tag="ACHOWS_004291"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFQ0049781.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQSLLNVPNEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6687..6780)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6757..6801)
                     /gene="trpL"
                     /locus_tag="ACHOWS_004292"
     CDS             complement(6757..6801)
                     /gene="trpL"
                     /locus_tag="ACHOWS_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFQ0049782.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6939..7820
                     /gene="rnm"
                     /locus_tag="ACHOWS_004293"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6939..7820
                     /gene="rnm"
                     /locus_tag="ACHOWS_004293"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFQ0049783.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HYGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7817..8437
                     /gene="yciO"
                     /locus_tag="ACHOWS_004294"
     CDS             7817..8437
                     /gene="yciO"
                     /locus_tag="ACHOWS_004294"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0049784.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8465..10354
                     /gene="yciQ"
                     /locus_tag="ACHOWS_004295"
     CDS             8465..10354
                     /gene="yciQ"
                     /locus_tag="ACHOWS_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFQ0049785.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVIRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAILREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            10567..11442
                     /gene="rluB"
                     /locus_tag="ACHOWS_004296"
     CDS             10567..11442
                     /gene="rluB"
                     /locus_tag="ACHOWS_004296"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFQ0049786.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHC
                     QVSGGRRSGGRNNNG"
BASE COUNT         2698 a         2911 c         3051 g         2787 t
ORIGIN      
        1 ggaaaaaaga cgtaagttga cgttcgacag gggcaagcga aacggtaaaa agataaataa
       61 taaatgaatt taggattttt ccagcttcgt taaagaaagt taaaatgccg ccagcggaac
      121 tggcggctgt gggattaact gcgcgtcgcc gctttcatcg gttgtacaaa agctttcagt
      181 gccgccagca ttttctctgg ctcattaata tgttgctcga tgattttaac gatggccgaa
      241 ccagaaatcg cgcccgcagc tcctgcatca atcgctgctt ttacctgatc cggggcggaa
      301 ataccaaagc cctgcaatgg aggcgcagca ttgtattctt tcagcttcgt gaccagatga
      361 ttgaggggta acgcggcgcg gttttctgcg ccggtcacgc ccgcacgtga caataaatag
      421 gtgtatccac gaccgtagga ggctatctgg cgcagcaaat catcgtcggc attcggcggg
      481 cagataaaga taggtgcaag attatgacgc aacgcggcct ggcggaaggg agcggactct
      541 tccactggca catcggcaac cagcaccgaa tcgacgccga ctttctcgca ctgggcataa
      601 aactcatcaa tgcctttgtt aaacaccagg ttggcataca tcagcaggcc aatgggaatg
      661 gtcgggtgtt tctggcgaat cagtgccagc atttcaaaac attgtgccgg agtcacacct
      721 gccgcaaagg cgcgcagagt ggcgttttga atcgtcgggc catccgccag tgggtcggag
      781 aaggggatac ctaactccag cgcgtcagca ccggcttcaa ttagcgtgtc gataattttc
      841 agcgactgct caatgcccgg atcaccgagc gtgacgaaag gaacgaatgc gccttctttg
      901 cgctccttca actgggcaaa cagagattcg tagcgttcca tcagatttcc cctcgtgctt
      961 tcaaaatatc gtgaacggtg aagatgtctt tatcgccgcg accggaaagg ttaaccacca
     1021 gtagctgctc tttttccggg ttttcgcgca tcattttcag cgcatgggcc agggcgtgtg
     1081 aggactccag cgccgggata atcccttcgt gcaggcagag cgttttgaag gcttccagtg
     1141 cttcatcatc ggtaattgac acgtaatcag cgcgtccagt gctgttaaga tacgcgtgtt
     1201 gtgggccgac agacgggaaa tccagtccgg cggagatgga gtaagattct tcaatctgcc
     1261 cgtcttcggt ttgcatcatc ggcgctttca taccgaaata gatgcccacg cgaccgtgtt
     1321 ttaacggtgc gccgtgctcg ccagtttcga taccgtgacc acctggctcc acaccaatca
     1381 ggccgacgtt ggtttcattg atgaaatcag caaacatgcc gatggcattc gaaccgccgc
     1441 caacacaggc gataacagca tccggcaggc gaccttctct ttccagaatc tgcgctttcg
     1501 tttcttcgcc aatcatccgc tgaaactcac gcacaatggt cgggtaagga tgcgggccag
     1561 ccgcggtgcc cagcatatag tgcgcggttt cataactgcc ggaccagtcg cgtagcgcct
     1621 cattacaggc atctttcagc gtcgcggaac cgctatgcac cgggatcact tccgcaccca
     1681 ttaaacgcat acgaaaaacg ttaggcgact ggcgttcaac gtctttggca cccatataaa
     1741 tgcgacattt caggccgagc agggcgctgg caagggccga cgccacgcca tgctgaccgg
     1801 ctccggtttc ggcgatgatt tccgttttac ccatccgctt cgccagcaac gcctggccga
     1861 gcacttggtt ggttttatgc gcgccgccgt gcagcaaatc ttcgcgcttg agatacagcg
     1921 tggtgttagt gccagccgta atgttctggc atttggtgag tgcggttgga cgcccggcat
     1981 agtttttcag caggtcgttg aactgagcct gaaattcagg atctttttgc gcgctaacaa
     2041 aagcttcttc cagctggcgc agagcaggca tcaggatttg tggcacgtac atgccgccaa
     2101 actcaccaaa atacgggtta agtaatgttg tcattgttcc tttccttaat atgcgcgcag
     2161 cgtctggaaa accgaggcca aaagacgtgc gtctttgatg cccggttgcg actctacagc
     2221 agaattaaaa tcaagtccgg cgcagccggt ttgtgccgct tccacgcagt tatctgcgcc
     2281 taagcccccc gccagcagaa cgttgccaag cgattgaccg tttaacagtg accagtcgaa
     2341 acgttgaccg cttccaccct ggccgttgtc taaaacatat ttatcaacgt gctgaagctc
     2401 gcgggcgggc agggtttcac cgacgcttaa tgctttccag atggcaacgt gtgctggcag
     2461 agcttcacgc agcgtatcga tatacagctg atcttcatta ccatgcagtt gcactgccgc
     2521 cagcgataac accttagctt tgtccaccac atcggcaata tcgtgattgc ggaacacgcc
     2581 aacatactgc aacggtgccg cagccatcac ttcctgcgcc tgttcaacgt tgacgcaacg
     2641 cggtgatgtc gcaacaaaaa tcaacccacc gtaaatcgcg cccgcgtcat aagctgcttt
     2701 agcatcttgc ccacgcgtca ggccacatac tttattctca cccagcaata cccggcgcac
     2761 ggcggcgttc aaatcgtcat gggccatcaa cgccgaacca atcagaaaac cgttagcgaa
     2821 gtggcttaac tcgcgcacct gagcgtaagt attgatgccg gattcgctga ttaccgtcac
     2881 gttgtgcccc agtttcggcg caagctcgcg ggtacggttg agatcaatcg acaaatcacg
     2941 cagatcgcgg ttgttgatgc caacgacctt tgcccccaat gcaatggcgc gctccagttc
     3001 ctcttcatta ctgacttcgg tcagcacacc catctccaga ctgtgggcga cggcggcaag
     3061 gtggcgatat tgttcgtcat ccagtactga aagcattaat aagcaggcat cggcctggta
     3121 atagcgcgcc agatagatct ggtaagggtc gataataaag tctttacata aaatcggctg
     3181 cggggcgatt tggctgacga tggagaggaa atcaaagctc ccctgaaaat atttctcatc
     3241 agtcagcacc gaaattgccg aagcgtaatt tctgtagacg gttgcaatgt gtgccggatc
     3301 aaaatcatcg cggatcacgc cttttgacgg tgacgctttt ttacactcca gaataaacgc
     3361 cgtgcgtgca ccctgtagcg catcataaaa atgtcgcgtg ctcggctgaa cctcattctg
     3421 aaaactggcc agcggttgct gctgtttgcg ggcttctacc caaatcgcct tgtctgcgac
     3481 gattttcgct aaaacggttt gcatcattta ccctcgtgcc gccagtgcgg tgactctgtc
     3541 gtaagcggaa ccactgcgca gtacctcaag aacggtttgc gcattggctt gcagatcttc
     3601 atggccatgc aggcgcatta acatggcgac gttcgccgcg acggctgctt catgggcggc
     3661 gtcgccttta ccttgtaaca agcgtgttaa aatgtcacgg ttttcttccg gtgttccgcc
     3721 tgccagttgc tcctggtggt agggtgtcag gccaaaatct tctgcagtta gctgatagct
     3781 cttaatttca ccgtcatgca gttcggcaac gattgtcggc gcgtgtaatg aaacttcatc
     3841 catcccgccg ctgtgcacta ccgcagcgcg ttgatacccc agcacgcgca aggtttcggc
     3901 aatcggcagc accagttccg gactataaac accaattagc gccagcggcg gatgcgccgg
     3961 gttaatcaat ggccccaaca cgttgaacag ggtgcgggtt ttcagttgct ggcgaaccgg
     4021 catcgcgtgg cggaatccgg tgtgatattt cggcgcaaag aggaaacaga cgcctaactc
     4081 atccagcgcc tggcgcgatt tatcggcgtt catatcaaga ttaataccga acgccgccag
     4141 cagatcggac gagccggatt tactggagac gctgcggtta ccgtgtttcg ccactttcag
     4201 cccacaggcc gcggcgacaa acgcactggc ggtagaaata ttgatgctgt tgctgccgtc
     4261 accgccagta ccgacgatat cggcaaacag ataatccggg cgcgggaacg gcgcggcgtt
     4321 ttccagtagc gcggttgctg ctccggcgat ctcgttcggg tgctcgccgc gaattttcat
     4381 gctcaccagc gccgccgcca gttgttccgg cttcagctcg ccacgcacca ccgctgaaaa
     4441 cagctggtga ctttcttgtt ggctaagcgt ctgtgcctga tacagttttt ccagaatcgg
     4501 ttgcagcgtg ttggttggct caagtttctg ctgcgcccag gccagcgttt gttccagcag
     4561 gcgagcgccc tgggtagtaa gaatggattc cggatggaac tggaaaccgc aaacgcgatc
     4621 tgcatcgtga cgcaccgcca tcaccatgcc attaaaatgg gcgttgatgg ttaaacctgc
     4681 cgggatatta ctgccaacca gcgagtgata acgcgccacc ggcaatgggt ttgttaatcc
     4741 ggcaaacatc gcctgactgt catgttcaat gctcgacgct ttaccgtgaa gaatttcgcc
     4801 cgcctgaccg acatagcccc cgtaagcttc gacaattgcc tgatgtccga ggcaaatgcc
     4861 aataattggc agcttgccac gcaagcgggt gaggagttcc ggcatacaac cggcttcgct
     4921 cggcacaccg gggccaggag aaagcatcag caccggattg ctcatcgtcg ccaggcgttc
     4981 aattaaggtc tgcgccggaa catggttgcg gtaaatcacc acgttatgac cattgctgcg
     5041 caactgatct gccaggttgt acgtaaaaga gtcgatatta tcgagcagca gaatgtcagc
     5101 catcagaacg tctcctgtgc atgatgcgcg gtggcaatag cgcgcagtac agcgcgggct
     5161 ttattacggg tttcgtcggc ttccgactgc ggaacagaat caaggactac gccagcaccg
     5221 gcttgcacgg tggcgatacc gttttccacc agcgccgagc ggatcacaat gcaggtgtcg
     5281 agatcgccat gcgcggtaaa ataacctacc gcgccgccgt agctgccgcg tcgacgacct
     5341 tctgcttcgg caattaactg catagcgcgc actttcggcg caccgcttaa tgtccccata
     5401 ttcatacagg cgcggtaagc gtgcagggcg tcgagatcgt ggcgcagctc accgaccacg
     5461 cgggagacga ggtgcatcac ataagaataa cggtcaactt tggtgagatc ggcgacgtag
     5521 cgactaccgg gagtgcaaat acgcgccagg tcattacggg cgagatccac tagcatcaga
     5581 tgttcagaaa gctctttatg atcggtacgc atttccagtt cgatgcggct gtcgaggtct
     5641 ctgtccagtg aaccatcggc acgacgaccg cgtggacgtg ttccggcaat cgggtagatc
     5701 tcaatctggc ggctggtggc gtcatacttg agcgaacttt ccggcgacgc gccaaacagg
     5761 gtgaaatcat tatcctgcat aaaaaacatg tacgggctgg gattactctt tttcagcacg
     5821 taataggccg ccagcggtga cgggcagggc agagagaaac ggcgagatgg caccacctgg
     5881 aaaatttctc cggcgcgaat cgctttttgc agcgaacgca ctacgccacc gaactcttca
     5941 tcactctggt tacattcaca acgcatatgc ggcacggaaa ccaccggcag cggcggcgcg
     6001 gcttcggtca gttgctgacg tagttcgttc aggcgagcag tgagacgttg tttttcttct
     6061 tcattcggag caaacaggct ggcctgaata cgggtgcttt ttttctgatg gtcaatcacc
     6121 atcagcgttt cagcgagata aaaacagaaa tcaggacagt tattttccgc tgccagttgt
     6181 ggtaaatctt caaatcccgc cacaaggtca taagagaaca gaccgccgaa gaacattgct
     6241 tctcgttctt cattcggtac attcaacaga ctctgcaata agcggaaagc gtcaaaaacc
     6301 gaaagggagc ataagcgagc gtcttcatcc agcagtggac tgacaggggg gaagcgcagc
     6361 acacggcagt ttggtgattg ttcattttcc acacccgcag gcagggcgtt atccagtagt
     6421 gccagcaggg cttcgccatt gccggaaagt gcctggattg tgacagtgtc acctaaagct
     6481 gtaatgcgca gcgcactgtc taccagcagc aggcttttta aatcatcttt gctgtcgata
     6541 tctgcggatt ccagtagcag cgttgctgga cgatcctcac acaactggtg aaaaagcgcg
     6601 gtgggattgt cgcgataagc gccttcgcag gttagcagtt cgagagtcgg tttttgtgtt
     6661 tgcattgtta ttctctaatt ttgttcaaaa aaaagcccgc tcattaggcg ggctgggtat
     6721 ctgattgctt tacgcatggt gaatacactg cccgtttcag gaagtgcgcc accaaccttt
     6781 cagtacgaaa attgctttca ttgtcgatac cctttttacg tgaacttgcg tactagttaa
     6841 ctagttcgat gattaattgt caacagctca tttcagaata tttgccagaa ccgttatgat
     6901 gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg aattacgcag
     6961 tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg ccagaagcat
     7021 tggtgcaccg tgcagtcgag atgcgcgtcg gcaccctggc gatcaccgac catgacacca
     7081 cagctgcaat ctcaccagca agagaggaaa tttcacgttc gggactggcg ctgaatctta
     7141 ttcccggcgt ggaaatttcc acggtctggg aaaatcatga aattcatatt gtcgggctga
     7201 atattgatat tactcatccg ctaatgtgtg agttcctcgc gcagcagaca gaacggcgca
     7261 atcagcgggc gcagctgatt gccgaaagac ttgaaaaagc gcaaatacct ggggcactgg
     7321 aaggcgcgca acgactggcg cagggcggtg cggtgacgcg cggtcatttt gctcgtttcc
     7381 ttgtggagtg cggcaaagcc agttcaatgg cggatgtctt taaaaagtat ctggcgcgcg
     7441 ggaaaaccgg atacgttccg ccacagtggt gtacaataga acaagctatt gatgtcattc
     7501 atcattctgg cggtaaggcg gtgctagctc atcctgggcg gtacaatctt agtgctaaat
     7561 ggctgaaaag attggttgcg cattttgccg aacactacgg cgacgcgatg gaagtcgcgc
     7621 agtgccagca atcgcccaat gaacgcaccc agctggcgac acttgcgcgt cagcatcatt
     7681 tatgggcatc acaaggatct gattttcatc agccatgccc gtggatcgaa ctgggtcgta
     7741 aactctggct gcccgcaggc gttgaaggcg tctggcagct atgggaacag ccgcagaaca
     7801 ccacagagag ggaattatga gccagttttt ttatattcat cctgataacc cgcagcaacg
     7861 cctgatcaac caggcggtgg agatcgtgcg taaaggcggg gtgattgttt atccaactga
     7921 ttccggctat gcgctcggct gtaaaattga agataaaaac gcgatggagc gtatttgtcg
     7981 aattcgccag ctgccggacg gtcacaactt taccctgatg tgtcgcgatc tttctgagct
     8041 ctcgacctat tcattcgtgg ataacgttgc gtttcgtttg atgaaaaaca acacgccggg
     8101 caactacacc ttcatcctga aagggacgaa ggaggtgcca cgccgtctgt tgcaggaaaa
     8161 acgcaaaacc atcggtatgc gtgtgccttc taacccgatt gcgcaggcgc tgcttgaagc
     8221 attgggcgaa ccgatgcttt ccacatcact gatgctgcca ggcagcgaat ttaccgaatc
     8281 cgatccggaa gaaattaaag accgtctgga aaaacaggtg gacttgatta ttcacggcgg
     8341 ctatctcggg cagaaaccaa caacggttat tgatcttact gatgatacgc cggtcgtggt
     8401 gcgtgaaggc gtaggtgatg tgaagccttt cttataacaa attgcgttcc acggatggaa
     8461 gactatggca gggatatatc gctgcatttt gctgttgata gttgggcttt ttttctcttc
     8521 gttgagttat ccgaaaaaca cggagatccc tttttatgaa gaagggatct cgctctttga
     8581 tgttgaagcc actctgcaac cggatggggt gctcgacatc aaagaaaata ttcattttca
     8641 ggcgcgaaat cagcagatta agcacggatt ttatcgtgat ttaccacgac tctggatgca
     8701 gcctgatggg gacgctgcac tgctgaacta tcatattgtt ggcgtcatcc gtgatggtat
     8761 tcctgaaccc tggcatcttg actggcatat cgggttaatg agtattgtcg tgggcgataa
     8821 acaacgtttc ttgcctcaag gcgactatca ttatcaaatt cattatcagg ttaaaaatgc
     8881 tatcctgcgt gagggggatt ctgatctgct aatctggaac gtgaccggta accactggcc
     8941 gtttgaaatt tataagaccc tattttcact caagttgcct gatattgcgg gtaatccatt
     9001 tagcgaaatc gatctcttta ctggagaaga gggcgacaca tatcgaaatg gccgcatcct
     9061 tgaggacgga agaattgaat ctcgcgatcc gttttatcgt gaagatttca cggtcctcta
     9121 ccgctggcct cacgcattac ttggtaatgc cccggctcca caaacgacga atattttcag
     9181 tcatcttctt ttaccctcaa cgtcatcgtt gttaatttgc tttccgagtc tgttcctggc
     9241 gtgtggatgg ttatatctct ggaagcgcag gccgcaattt acgccggtag atgtgattga
     9301 aaccgatgtc attccgccag attacacgcc cggcatgtta cgtcttgatg cgaaactggt
     9361 ttacgacgat aaaggttttt gcgccgatat cgtaaatctg attgtgaaag aaaaaattca
     9421 tctggaagat cattatgaca agaaccagca aatcctgatt cgtgttaatg aaggcgcgac
     9481 cagaaataat gcggtattac tgcccgcaga gcagttatta ctggaagcgt tatttcgtaa
     9541 aggcgataag gtcgttctta cggggagacg caacagagtc ttacgcaagg catttttacg
     9601 gatgcagaaa ttttatctgc cgcgtaaaaa gtcttcgttt taccgacctg atgcgttttt
     9661 gcaatggggc ggaatggcaa tattggcggt cattctctac ggtaacctga gtcccgtagg
     9721 ctgggaagga atgagtctgg tgggcgatat gtttattatg atctgctggc ttcttccttt
     9781 tttattttgt tcccttgagc ttttgtttgc ccgcgatgat gacaagcctt gcgttaatcg
     9841 tgtcatcatc actttgtttt taccactgat ttgttcaggt gtggcttttt actctctcta
     9901 catcaatgtc ggagatgtat tcttttactg gtatatgcca gcgggttatt ttagcgctgt
     9961 tttcctgacc ggttatctca ctggcatggg gtatattttt ctgccaaagt ttacccaaac
    10021 tgggcagcaa cgttatgccc acggtgaagc tatcgttaac tatcttgcgc gtaaagaggc
    10081 agcaacacac agtgggcggc gtaggaaagg ggaaacacgg aaactggatt acgctttgtt
    10141 aggttgggct atatcggcaa atttggggag ggaatgggcg ttacgcatcg ccccttcgct
    10201 ttcttcggcg atttgcgctc cagagattgc ccgtaacggc gttttattct cattacagac
    10261 gcacctgagt tgcggggcct ataccagttt gttggggcgg agctattctg gtggtggttc
    10321 tggcgctggt ggcggaggcg gtggtggctg gtaatcaagc tgatgttaat cgaaacaagc
    10381 tttaaccata aagatagtgt agtatgttgc gcctcaaagc caggcaagtt aaaacgtcga
    10441 gtcgtttact taaggcctga agagttcaaa cagactttac tatcagtgag tcagatgtaa
    10501 attctgactc actgttgcac aaaagcatat cagacgcctg ggaaggcgac accaaaagga
    10561 agctctatga gcgaaaagct acagaaagtg ctggcgcgtg ccggccacgg ttctcgccgt
    10621 gaaatcgaat ctattattga agccggtcgt gtgagtgttg atggcaaaat tgctaaactc
    10681 ggcgatcgcg ttgaaattac ccctggattg aaaatccgta tcgatggtca cctgatttcg
    10741 gtacgtgagt ccgctgaaca aatttgtcgc gttctggcct attacaaacc ggaaggtgag
    10801 ttgtgtaccc gtaacgatcc ggaagggcgt ccaacggtgt ttgaccgtct gccaaagctg
    10861 cgtggcgcac gctggattgc cgtaggtcgc ctggacgtta atacctgtgg tctgctgctg
    10921 ttcactaccg acggtgaact ggcaaaccgt ttaatgcatc caagccgtga agttgaacgt
    10981 gaatacgccg tgcgcgtatt tggtcaggtt gacgacgcga aactgcgtga tttgagtcgt
    11041 ggcgtgcagc tggaagatgg tccggcagcc tttaaaacca tcaagttcag cggcggcgaa
    11101 gggatcaacc agtggtacaa cgttactctg accgaaggcc gtaaccgtga agttcgtcgt
    11161 ctgtgggaag cggttggtgt gcaggtaagc cgcctgatcc gtgttcgtta cggtgatatc
    11221 ccactgccga aaggtctgcc acgcggtggc tggacggagc tggacctcgc ccagactaac
    11281 tatctgcgcg aactggtgga gctaccgcca gaaaccagct ctaaagtcgc tgtagaaaaa
    11341 gaccgtcgtc gtatgaaggc gaatcagatt cgtcgtgcgg tgaaacgtca ctgtcaggtg
    11401 agcggcggtc gtcgttctgg tggacgtaat aataacggtt aatttaa
//
LOCUS       DAZKJR010000154        11415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKJR010000154 DAZKJR010000000
VERSION     DAZKJR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.155"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..423
                     /locus_tag="ACHOWS_004297"
     CDS             <1..423
                     /locus_tag="ACHOWS_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFQ0049787.1"
                     /translation="PGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASL
                     NDEQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFR
                     ALAAELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            438..1295
                     /gene="menB"
                     /locus_tag="ACHOWS_004298"
     CDS             438..1295
                     /gene="menB"
                     /locus_tag="ACHOWS_004298"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFQ0049788.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1295..2257
                     /gene="menC"
                     /locus_tag="ACHOWS_004299"
     CDS             1295..2257
                     /gene="menC"
                     /locus_tag="ACHOWS_004299"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFQ0049789.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSGLLAWVNNWLAGDSELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALERLL"
     gene            2254..3609
                     /gene="menE"
                     /locus_tag="ACHOWS_004300"
     CDS             2254..3609
                     /gene="menE"
                     /locus_tag="ACHOWS_004300"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFQ0049790.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMVEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLNLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3719..3985
                     /gene="pmrD"
                     /locus_tag="ACHOWS_004301"
     CDS             3719..3985
                     /gene="pmrD"
                     /locus_tag="ACHOWS_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFQ0049791.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(3979..4365)
                     /gene="arnF"
                     /locus_tag="ACHOWS_004302"
     CDS             complement(3979..4365)
                     /gene="arnF"
                     /locus_tag="ACHOWS_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFQ0049792.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4365..4700)
                     /gene="arnE"
                     /locus_tag="ACHOWS_004303"
     CDS             complement(4365..4700)
                     /gene="arnE"
                     /locus_tag="ACHOWS_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFQ0049793.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4697..6349)
                     /gene="arnT"
                     /locus_tag="ACHOWS_004304"
     CDS             complement(4697..6349)
                     /gene="arnT"
                     /locus_tag="ACHOWS_004304"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFQ0049794.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGAMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6349..7239)
                     /gene="arnD"
                     /locus_tag="ACHOWS_004305"
     CDS             complement(6349..7239)
                     /gene="arnD"
                     /locus_tag="ACHOWS_004305"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFQ0049795.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7236..9218)
                     /gene="arnA"
                     /locus_tag="ACHOWS_004306"
     CDS             complement(7236..9218)
                     /gene="arnA"
                     /locus_tag="ACHOWS_004306"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFQ0049796.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LTVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9218..10186)
                     /gene="arnC"
                     /locus_tag="ACHOWS_004307"
     CDS             complement(9218..10186)
                     /gene="arnC"
                     /locus_tag="ACHOWS_004307"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFQ0049797.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10190..11329)
                     /gene="arnB"
                     /locus_tag="ACHOWS_004308"
     CDS             complement(10190..11329)
                     /gene="arnB"
                     /locus_tag="ACHOWS_004308"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFQ0049798.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKSTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2778 a         2970 c         3065 g         2602 t
ORIGIN      
        1 cctgggttgc aaaatgctga acaacgcacg gaacgtcagc gttccgatcg ccaatgggcg
       61 cagcgttttc gcacagaacc gttaacggcg gtatttgctg actggtatca acagcctgtt
      121 tttgcctcac tcaatgacga acaacgccgg gagctggttg cgctgcgcag caacaataat
      181 ggcgcaacac ttgccgccat gctggaggcg acttctctcg ccgtccagcc cgatttacgt
      241 gctaacctta gcgcccgcac atttgcgttt tattatttat gtggtgaacg tgacagcaaa
      301 ttccgcgccc tggcggcgga actggctgcc gaatgccatg tcattcctcg cgccggacat
      361 aacgcgcacc gggaaaatcc cgctggcgta atcgcaagtc tggcgcagat cttgcgtttc
      421 tgactaaagg acacaatatg atttatcctg atgaagcaat gctttacgca ccggttgaat
      481 ggcacgactg ctccgaaggt tttgaggaca ttcgttatga aaaatccacc gacggtatcg
      541 caaaaatcac cattaatcgt ccgcaggtgc gcaatgcctt ccgtcctctg acggtaaaag
      601 agatgatcca ggcactggca gatgcgcgtt atgacgacaa catcggcgtg atcattctga
      661 ctggcgcagg cgataaagcg ttctgctccg gcggtgacca gaaagtgcgt ggtgattacg
      721 gcggctataa ggatgattcc ggcgtacatc acctgaacgt gctggacttc cagcgtcaga
      781 tccgtacctg cccgaaaccg gttgtcgcga tggtggctgg ctactccatt ggtggcggtc
      841 acgttctgca catgatgtgc gacctgacta tcgcggcaga taatgccatc ttcggtcaga
      901 ctggcccgaa agtcggttcc ttcgacggcg gctggggcgc ttcctacatg gcgcgcatcg
      961 tcgggcagaa aaaagcgcgt gaaatctggt tcctgtgccg tcagtacgac gcgaaacagg
     1021 cgctggatat gggccttgta aataccgtgg tgccgctggc ggatctggaa aaagaaaccg
     1081 tgcgctggtg ccgtgaaatg ctgcaaaaca gcccgatggc gctgcgctgc ctgaaagctg
     1141 cactgaacgc cgactgtgac ggtcaggcgg gtctgcagga gctggcgggc aacgccacca
     1201 tgctgttcta catgacggaa gaaggtcagg aaggtcgcaa cgctttcaac cagaaacgtc
     1261 agcctgattt cagcaaattt aaacggaatc cgtaatgcgt agcgcgcagg tataccgctg
     1321 gcagatcccc atggacgcgg gggtggttct gcgcgacagg cggttaaaaa cccgcgacgg
     1381 gctgtatgtt tgcctgcgtg agggcgagcg cgaagggtgg ggggagatct ccccactacc
     1441 gggcttcagc caggaaacct gggaagaggc acaaagcggg ctgcttgcct gggtaaataa
     1501 ctggctggca ggtgatagcg agctaccgca aatgccttcc gtcgcctttg gcgtaagctg
     1561 tgcattggcg gagctggcag acacgctccc gcaggcggcc aattatcgtg cggcaccact
     1621 gtgtaatggc gatccggacg atctgatcct gaaacttgcc gatatgccgg gcgagaaagt
     1681 ggcgaaggtc aaagtgggat tgtacgaagc cgtgcgcgac ggcatggtgg tcaatctgtt
     1741 gctggaggca attccggatc tgcatttgcg tcttgacgcg aaccgcgcct ggacgccgct
     1801 gaaaggccag cagtttgcca aatacgttaa cccggattat cgcgaccgca tcgcgtttct
     1861 cgaagagccg tgcaaaaccc gcgatgattc ccgtgcgttt gcccgtgaaa ccggcattgc
     1921 cattgcctgg gatgaaagcc tgcgcgagcc ggattttgcc tttgtggctg aagagggcgt
     1981 gcgcgcggta gttatcaaac ccacgctcac gggcagcctg gagaaagtac gcgagcaagt
     2041 gcaggcggcg catgcgctgg ggctgacggc ggtgatcagt tcttccattg agtcgagctt
     2101 aggtctaacg caactggcgc ggattgctgc ctggttaacg ccagacacca ttccgggact
     2161 ggacacgctg gatctgatgc aggcgcagca ggttcgtcgc tggccgggga gcctgctgcc
     2221 gttagtggac gttgatgcac tggagcggct gttatgatct tctctgactg gccgtggcgt
     2281 cactggcggc aagtgcgggg agaagccatc gccttacgtc ttaatgacga gcaactcaac
     2341 tggcgcgagc tttgtgctcg cgtcgatgaa ttagcctcca gctttgcgat gcagggtgtg
     2401 gttgagggca gcggcgtgat gttgcgggcg tggaatacgc cgcaaacgct gctggcctgg
     2461 ctggcgttac tgcaatgcgg ggcgcgggtg ctgcccgtga atccacagct gccgcaaccg
     2521 ttacttgagg aattgctccc caatcttacg ctgcaatttg cgctggtgcc ggatggggaa
     2581 aacacgtttc cggcattaac gtcgctgcac attcagcggg ttgaaggcgc acatgccgct
     2641 acgtggcagc cgacgcgtct gtgctcaatg acgttgacct cgggttctac cgggctgccg
     2701 aaagccgctg tccatactta tcaggcccat cttgccagtg ccgaaggggt gttatcgttg
     2761 attccgtttg gcgatcacga tgactggttg ctctctttac cgctgtttca cgtctccggt
     2821 cagggaatta tgtggcgctg gttatacgct ggtgcgcgga tgacggtgcg tgataaacag
     2881 ccattggagc aaatgctggc aggctgtact cacgcttcac tggtgccaac acaactctgg
     2941 cgtttgctgg ttaaccgcag ttccgtttcc ctgaaagcgg tgttacttgg cggcgcggct
     3001 atcccggtcg agttgacgga acaggcgcgc gagcagggga ttcgttgctt ttgcggctat
     3061 ggtctgaccg agtttgcctc cacggtgtgt gcgaaagaag ccgacggtct ggcagacgtt
     3121 ggttcgccgc tgccgggtcg ggaagtgaaa atcgttaatg atgaagtgtg gctgcgggct
     3181 gctagtatgg tagaaggtta ctggcgtaac gggcaactgg tttcactggt taatgacgaa
     3241 ggctggtacg ctacgcgcga tcgcggtgag atgcataatg gcaagctgac cattgtcgga
     3301 cgtttagaca atctattctt cagtggcgga gagggtattc agccggaaga agtcgagcgc
     3361 gtaattgctg cacatcctgc ggttttgcag gtgtttatcg tccccgttgc cgacaaggag
     3421 tttggtcatc gaccggtggc ggtggtggag tatgaccagc aaaccgttga tcttgacgaa
     3481 tgggtgaaag acaagctggc tcgttttcaa caaccggtgc gctggctgaa tctgccgccg
     3541 gagctgaaaa acggcggtat taaaatttca cgtcaggcgc taaaagagtg ggtgcaacgc
     3601 cagcaataat taaccctccg tttcacttct catttctgcc attccattgc cgttgataaa
     3661 gagtttatct gttaatctgt aaattgatgt gaaaactctt agcaaacagg ataatgcaat
     3721 ggaatggctg gtcaaaaaat cgtgctgcaa taaacaagat aacagacatg taattatgct
     3781 ctgcgatgct ggcggcgcga taaaaatgat cgccgaagtg aagagtgact tcgccgtgaa
     3841 agtgggggat ttactctcgc ctttgcagaa tgcgctttac tgtattaatc gtgaaaagct
     3901 gcacacggtg aaagtccttt ctgcaagctg ttatagcccc gatgagtggg aacggcagtg
     3961 taaagcggca gggaaaactc agtaacgttg ttttgttgtg ggcaggaaaa tcagcatcaa
     4021 cccgctcata atacaggcta ctcccagtag tgctttcagc gaaaaagtcc cttcccagcc
     4081 gggaagtacc attgaagcaa tccataccag cacataactc atactgagca aggcataggc
     4141 tttgcttagt gcaagtttat gtagtgtttt ataccaacaa aaaacggaaa gcagataacc
     4201 cagcagcccg agtaacagta tccgggcatc caggccaaaa gctaacaacg ccgcgataaa
     4261 atcccataag tgcgtcatcg gcggcagatg actcgccgca aaacccaggc ttaattgcgc
     4321 agcggaggcg ataatgacgc tgaataatcc ccacatcagg cccattacac ggtactcccg
     4381 aggatcacaa tgccaccaat aatgaacgcc accccacacc agtgacgcgg cgataccggt
     4441 tcgtgccaca gttttactgc agccagcgtc acccagacaa aattcagact taacatcggg
     4501 taggcaatgc ctaccggtac gttctgcaag accagcagcc agagcaccat ggcaagacca
     4561 agacaagcca gcgccagtcc cagccacagc acgatatgtt tgcgccgttt gtttatcgcc
     4621 acaaagcagg ttgcctgttt ctgacacaac tgcccggcaa cgctaagcaa gctggcaaag
     4681 actaatgtta gccagatcat ttgggacgat actgaatcag caccagacgc tcctgacgat
     4741 cgatggcatc tgcgggcgga atggcgagac tgttgatatc ttcattgcgg tcaaccgaaa
     4801 gcacgagtgt aataatcccc tcctgacgat gttgattaag ccagtttgcg aactcatcac
     4861 cgctgacaaa tctccctttc gcatccggat aattaaggcc gtatttcaac tcacctgtct
     4921 ggcgatacat gatgatgtca tcgcgttgca ggctccatgc cagacctgcg gcaacaccga
     4981 cgctatcagt aagaatatag cggcttggct gaagtgattc ttgtgtcatc tcgacaaaaa
     5041 attgcggatg ttttccttcc atgactctgt caggaattga aaatcctacc agcaacgcca
     5101 gccccagcgg gcaaagcgcg gcaaaagacc aggtcttttc gacgtttgtt aaggtgtacc
     5161 agccgaaaaa tgcccatagc gaaaaaatcg accaggcaca aaagacttta tagctctcga
     5221 aggtttgcca caccggcgtg ttcattgctc cccaggggga gaccacaaat gtggcaataa
     5281 tgccagtgac gccaaaagcg atgttaatcc agccattaat ccgcaacgcc agaggattat
     5341 tttttgctgc cagcaaagcg taatgcgcca tcagcattgc cagaggagca aagcaagaaa
     5401 gaatataggt gggcaatttg cctttggcga cggagaaaaa caacagcggc attatcgtcc
     5461 agctcaacaa atagacggtt gcggaatgct tgcggttttt ccagcctgcg tacagtgctc
     5521 cggggagtaa tcccagccac ggcaggctac cggcaatgat gaccggcaag tagtaccaga
     5581 acggagctct atgttgggca tcatccattg caaagcgttg aatatgctca acccagaaaa
     5641 aatagtgcca gaagtcaggc tcccgctgcg ctatcgccag tccccagggg agaaccgtca
     5701 gtacgcaact gatgaccgcc agccagccgt aaataaagag atctttccag cgtttttgcg
     5761 ttgcaaccca tggaagcacg cttaataccg gcacggcaag ggcgagaaaa cctttggtca
     5821 tcacccccat gccgcaagtg attcccagca gtaaaaatcc cgcgcttttg cctttccacg
     5881 tctgtgcctg cattgccagc cagaagctgc acattcccgc caccagccag aaggcaataa
     5941 acggatcgag cacggcataa gtgccaatgg catagacaat aaacaatgag agataaatca
     6001 ctgcggcgag tagagccaga cgtttatcgc gccataagcg cagagtaaac caggtcacca
     6061 gcgcggcagt taacagggtc gcaaagataa cgcctgcccg cacaccaaag ttattcgcgc
     6121 caaatagcca ttgcccaatg ctgttaatcc agtatccggc aatgggtttt tcgaaataac
     6181 gtagccctaa cagatggggc acaatccagt cgccggatgc cagcatttct cgactgattt
     6241 ccgcataacg cgtttcatcg ggttgccata gcagacgcgt gctgatcggt aacaggtaat
     6301 agcaggcaat aaacgcgaag aggccgataa ggtaacgtac cgatttcatc agcgactacc
     6361 cgcaatttgt tggcaaccca gccagccttc acgtccggca atatttccgc gaacaacttg
     6421 tccgagcggc aacatccctg ataacagttc gcttaaaggg caaaatgtca cgccttcctg
     6481 ggctgcccgt ttaaggagat ccacaaaatt atgctgataa gcgcagcctt caacttctgc
     6541 atgaatggta taaaccggcg tgcctttatc tcgcaggatg cgattgagta accaaccgtt
     6601 aaaatcttct gctttcacat cccgaccaat cacttcatcc caggtgggta aggtcaccgg
     6661 aatttgcgca gtaccagggt ttcctgattc gagcagtgga cgaaacggca tcgccccacg
     6721 acaatcgctg ttgtagcgca aatggaacgc ttcttttgct tcgatcacct gctggtcggc
     6781 acgccagcca gcggctgcgg agcaggttac cggttggccg ataatttctt ccagggtgcg
     6841 aaggccgcgt gcgatatcgt cgatcattgt ttgccgatcc caattaccgc tatgggcttg
     6901 ccaggcatgg tgatcccagg cgtgcaggcc gacttcgtga tgtttagccg cttcacgaat
     6961 gatatcggca ttggcatgac caatctcttt acccggccag gccgtacctg ccagtaaaat
     7021 atcccagcca taaagcgatg ccgcgtttga gcgcagcatc ttccacaaaa actgtggctt
     7081 caccagtcgc cagagatggc ggcccatatt gtccgggccg acgctgaaaa aaatgctggc
     7141 ctgaatatta tgcttactca agatttccag cagacgcggc acgccttcac gggtgccacg
     7201 aaaggtatcg acatcaatgc gtaagcctac tttggtcatg atggtttatc cgtaagatca
     7261 acggtgcgca ggaagaaatc cagcgtttcg tcgatggttt cctgcatatc aattttcggt
     7321 tcccagtcca ggcagcggcg ggcattgcgg atgctcggtt tacgatgctc tacgtcctga
     7381 tatcctttgc catagtagct gctactttcg acaacacgaa agcctgcaaa cggcgggaag
     7441 tgatggcgca gcggatgttt ttcgaagctc gccagcagca tctcgcccag ttcctcaatg
     7501 ctcgcttcgt tctcaggatt gccaatgttg ataatttcgc cgtcgcagcg atttcccgca
     7561 ttttcgataa tgcggtacaa cgcctcgata ccatcccgga tatcagtaaa gcagcgtttt
     7621 tgttttccgc catcaattag cttaattggt gaaccttcta ccagattgag aatgagttgc
     7681 gtaatagcgc gggagctgcc aattcgcgct gcattaaggt tatccagtcg tggtcccatc
     7741 cagttaaacg gccggaagag ggtgaactgt aaaccctctt tttcgccata ggcccagatc
     7801 acccgatcaa gtaattgttt tgacaccgaa taaatccagc gtggtttatt caccgggccg
     7861 acggttaaat tagaatggtc ctcatcgaag tatttatcgc tacacatccc ataaacttct
     7921 gaagttgacg ggaagatgat tcgcttacgg tacttcacgc agtagcggat aatgcgcaga
     7981 ttctcttcaa aatcgagttc aaatacgcgc agcgggttgc gggtatattc aatcggcgtg
     8041 gctatcgcca ccagcggcaa gacgacatca cattttttga cgtgatactc aatccattcg
     8101 gaatgaatac tgatatcgcc ttcgacaaag tgaaaatgcg gatgattcag aaaacggctt
     8161 atcgcatcgc tgccaatatc cagaccgtaa acttcataat gatcttcgcg cagcaggcgt
     8221 tctgtcagat ggttgccaat aaagccattc accccgagga tgagtacccg ggtacggcgt
     8281 cgggcggcac aggcaggctg gctattcaag cgtgaacctt gcaccaggcc cagcgtctgc
     8341 gctaattgcg agccctgcat agtaatgccg tcgcccgcct gtccggtgac gatttccagc
     8401 gcgccatcgc cacaggcaat cagcagtggc gctacagaaa tcacgctccc cggctgtgct
     8461 ttgctggcat gaggatgaac acgcgacgac cagacggtga atttctgatt gccaacatag
     8521 ctgaaggcac ccggccacgg atcggcaacg gcacgtacca tgttgtgcag tacggatgcc
     8581 ggcttatgcc attcgaggaa actgtcatcc ggcgttctgc gaccaaaaca ggtagcttcg
     8641 ttttcgcgct gggcgatttc cagaatatta ccgtgtttaa tggtgggtaa tgtctgctcc
     8701 agtagctggc gcgcggcatg acataactta tgatgcaacg taatggcgat atcgtctggc
     8761 gcaatggcaa tgcgcagttg cgccacaatg gccccggcat cggcacgttt caccattcgg
     8821 tgcaatgtaa cgccagtttc cgtttcaccg ttgaccagca cccagttcag cggcgcgcga
     8881 ccacgatatt ttggtaacag cgaaccatgc agattaaatg cacctgcggg agcgagctgc
     8941 aaaatctcgt cgcaaataag atggcgataa taaaaagaga aaatcacctc tggtgagagt
     9001 tgggcaatac gttccaccca cagcggatga ttaacgttat ctggcgcata aaccggaatg
     9061 cctctttccg ccgccagacg agctaccgaa ccataaaagg ctttttcacc gggattatcg
     9121 gtatgagtaa aaatagcgct aatttcgtaa ccggcagcca gcagggcttc aataccgagg
     9181 catcccatat cgtggtaggc aaaaacgacg gttttcatta ttcattttcc ttgctggatg
     9241 gacggataac ttgctgaaca aaatagcggg ggcgggcgcg gacatcggtg tagatcctgc
     9301 cgatatattc accgagtaat cccatgccga taaactgagc gccaataaaa gtaaacagca
     9361 cggcaaatag cataaagacg ccttctgccg cccattgtgg tccgaaggtc aggcgtaaaa
     9421 tcaccagcag caccgcaatg ctaaaacctc caatcgcaat aatgctgccg agcagactca
     9481 gcatacgtag cggcgttgtg gtaaggcagg tcaccaggtc gtacatcaaa ttaatcaggc
     9541 gcataaaact gtatttggat tcaccaaact cacgctcggc atggtgcact ggaatttcaa
     9601 tggcacggcg ggcgaagata ttcgccagaa tcgggataaa ggtactgcgt tcatggcagt
     9661 gcaacatcgc atcgacaata tgacggcgat aggcgcgcag catacaaccg tagtcaccca
     9721 tcgctttgcc ggtggtgcgc tgaataagcc ggttaatcat cttcgaagcg gttttacgaa
     9781 accagctgtc ctggcggttc tggcgtacag tccctaccac gtcgtaacct tcatcggctt
     9841 ttgccaccag acgggggatt tcctctggcg gattctggag atcggcatcg agggtaataa
     9901 ttaagtcgcc agtaacgtga ctgaatcccg ccataatcgc tgaatgttgc ccgtaattgc
     9961 ggttaagcaa aatagacaca atatggctgt tctccgcttg tgaggcttcg accagcatat
    10021 gcgcggaatt atcgctactg ccgtcatcaa tcagcaggat ttcatactct ttccccagtg
    10081 attcacaggc tgcggtggtg cgcctgatta attccggtaa gctttcctgc tcgttataaa
    10141 cgggaataac caccgagact ttcttaacag ggtggatttc aaacatggct tattgtcctg
    10201 cgagttgctg aagggctgtg atgacgcggt cggcatcggc ggtggtcata tccgggaaca
    10261 gcggcaacga acagatgcgt tcgctattcc attcggtatt cggtaacgac agcgtgggaa
    10321 aacgctcgcg ataatatttt tgtgtgtgag cggcgcggaa atgtaacccg gtaccaatgc
    10381 ctctttcttt taacgcttcc atcaacgcat cgcgactgat accacaacgt tgttcatcga
    10441 cacgaataat aaacagatgc caggcgtgaa cgtgtgacca ggcgggaagg cttaatggct
    10501 gaaaggggag agctgccagt gcttgctgat attgctgggc aatttcgcgc cgacgggtgt
    10561 tgaggtgctc taatttgact aactgtgtca gggcaatcgc ggcgttaata tcggtcagat
    10621 tgtacttata gcccggcgtt aagacttcag cctgcggtgc acggccccag gtatgtctgt
    10681 cataggcatc gacgcccaga ccgtgaaatt ttagcatccg tagctggcgg gcaaggtttt
    10741 cattatctgt aacaatcagg ccaccttccg cacaggtaat atttttgatg gcgtgaaacg
    10801 aaaaaatagc ggtacttttt gcgccaatat gtcgcccttt gtaatacgta cctacggcat
    10861 gggcagcatc ttcgataact gcgatgccgt aacgttcgcc aatggcgcga atggcgtcaa
    10921 tatctgctgg cgcaccggca taatgcaccg gaatgatggc tttagtgcgt ggcgtaatgg
    10981 ctgactcgat cgcttcaggc gtgaccatca gcgtatcgcg gtcgacatcc accattaccg
    11041 gcgttgcacc caacaaggag atcatattga gggttgaaac ccaggtcagg gaaggcgtaa
    11101 tcacttcatc gcccttgcca atttccaacg ccattagcgt gatatgcatt ccggcggtgg
    11161 ctgaactgac cgcgatggca tgctgatttc ccgtcaactg gcaaaaggct tgttcaagcg
    11221 cctgattctt cggaccggtt gtgatccaac cggattcgag aacctcttta actgctgcga
    11281 gttcctccac gcccattgct ggtcgcgaaa aaggcaaaaa ttctgacatt gcttttcctt
    11341 ccgccattga atacctgtcc acttatattt gctataaaga gtgttgtgta tattttgcca
    11401 tttggagcga aattc
//
LOCUS       DAZKJR010000155        11348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKJR010000155 DAZKJR010000000
VERSION     DAZKJR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.156"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            227..526
                     /gene="yoaC"
                     /locus_tag="ACHOWS_004309"
     CDS             227..526
                     /gene="yoaC"
                     /locus_tag="ACHOWS_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFQ0049799.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(646..825)
                     /gene="yoaH"
                     /locus_tag="ACHOWS_004310"
     CDS             complement(646..825)
                     /gene="yoaH"
                     /locus_tag="ACHOWS_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFQ0049800.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HAGERIVARFEDEDE"
     gene            899..2260
                     /gene="pabB"
                     /locus_tag="ACHOWS_004311"
     CDS             899..2260
                     /gene="pabB"
                     /locus_tag="ACHOWS_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFQ0049801.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSENEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEGFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHTSDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2263..2841
                     /gene="nudL"
                     /locus_tag="ACHOWS_004312"
     CDS             2263..2841
                     /gene="nudL"
                     /locus_tag="ACHOWS_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFQ0049802.1"
                     /translation="MEHRSLTLDDFLSRFQLLRPQMNRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3025..4389
                     /gene="sdaA"
                     /locus_tag="ACHOWS_004313"
     CDS             3025..4389
                     /gene="sdaA"
                     /locus_tag="ACHOWS_004313"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFQ0049803.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4520..6118
                     /gene="pdeD"
                     /locus_tag="ACHOWS_004314"
     CDS             4520..6118
                     /gene="pdeD"
                     /locus_tag="ACHOWS_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFQ0049804.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDHALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLLRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6122..7678)
                     /gene="yoaE"
                     /locus_tag="ACHOWS_004315"
     CDS             complement(6122..7678)
                     /gene="yoaE"
                     /locus_tag="ACHOWS_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFQ0049805.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7666..7824)
                     /gene="yoaL"
                     /locus_tag="ACHOWS_004316"
     CDS             complement(7666..7824)
                     /gene="yoaL"
                     /locus_tag="ACHOWS_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFQ0049806.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8141..9112
                     /gene="manX"
                     /locus_tag="ACHOWS_004317"
     CDS             8141..9112
                     /gene="manX"
                     /locus_tag="ACHOWS_004317"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFQ0049807.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKATPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9175..9975
                     /gene="manY"
                     /locus_tag="ACHOWS_004318"
     CDS             9175..9975
                     /gene="manY"
                     /locus_tag="ACHOWS_004318"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFQ0049808.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9988..10839
                     /gene="manZ"
                     /locus_tag="ACHOWS_004319"
     CDS             9988..10839
                     /gene="manZ"
                     /locus_tag="ACHOWS_004319"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFQ0049809.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10894..>11348
                     /gene="yobD"
                     /locus_tag="ACHOWS_004320"
     CDS             10894..>11348
                     /gene="yobD"
                     /locus_tag="ACHOWS_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFQ0049810.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVST"
BASE COUNT         2779 a         2871 c         2850 g         2848 t
ORIGIN      
        1 gcatctctgg ttttctttct tacctgaaca atctggttat agcgtgagga ctgaccatta
       61 attttctcca tcttactgga taacacacta tgagctgcta cctttaaaac acctgaatgt
      121 caacaggtta actcgtgcca tatcgttata atccctttga tattagatac aaattaaggt
      181 catatatagc cttatcgata acatggttaa ttttaaggat aagaatatgc ctgctgtaat
      241 agataaagcc ctggatttca ttggtgccat ggatgtatca gcgccaacac caagttcgat
      301 gaatgaaagt acggcgaagg gaatctttaa atatttaaaa gaactgggag tacccgcgag
      361 tgccgctgat attacggcgc gagctgacca ggaaggctgg aaccctggtt tcacggaaaa
      421 aatggttgga tgggcaaaaa aaatggagtc tggtgaacgc tctgtgatta aaaatcctga
      481 atacttttca acatatatgc aggaagaact aaaagcactg gtttgagtta aatttacatc
      541 agcataaatg ggtcaggacg cttttaatca catataaaaa gcgtcctttt tctcacaatc
      601 aaataatcat cggcttctat aacgaaagcg atacggatta gtgtgttact cgtcttcatc
      661 ctcaaaacgc gccacgattc gctcgccggc atggttggca cgcagctctt ctgccaccaa
      721 cgcgattgcc tggccgctgc tcatcccttg agccatcagt tcctggatcc gctcgacagc
      781 tttttgctgc tgttcatggg tgagtgaagg taaacctgca aacattgtta actcctgcta
      841 aattgttggc gctaattatt tcatgctacc cggcacatag ccagtagagt caggactgat
      901 gaagacgtta tctcccgctg tgattacttt accctggcgt caggacgccg ctgaatttta
      961 tttctcacgc ttaagccacc tgccgtgggc gatgctttta cactccggct atgctgatca
     1021 tccatatagc cgctttgata ttgtggtcgc cgagccgatt tgcactttaa ccactttcgg
     1081 taaagaaacc gttgttagtg aaaacgaaaa acgcacaacg accactgatg acccgctaca
     1141 ggtgctccag caggtgctgg atcgcgcaga cattcgccca gcgcataacg aagatttgcc
     1201 atttcagggc ggcgcgctgg ggttgtttgg ctacgatctg ggccgccgtt ttgagtcact
     1261 gccagaaatt gcgcagcaag atatcgttct gccggatatg gcagtgggta tctacgactg
     1321 ggcgctggtt gttgaccacc agcgtcaaac agtttctttg ctgagtcata atgatgtcaa
     1381 tgctcgtcgg gcctggctgg aaagccagca attctcgccg caggaaggtt tcacgctcac
     1441 ttccgactgg caatccaata tgacccgcga gcagtacggc gaaaaatttc gccaggtaca
     1501 ggaatatctg cacagcggtg attgctatca ggtgaatctc gcccagcgtt ttcatgcgac
     1561 ctattctggc gatgaatggc aggcattcct tcagcttaat caggccaacc gcgcgccatt
     1621 tagcgctttt ttacgtcttg aacagggtgc aattttaagc ctttcgccag agcggtttat
     1681 tctttgtgat aatagtgaaa tccagacccg cccgattaaa ggcacgctac cacgcctgcc
     1741 cgatcctcag gaagatagca aacaagcaga aaaactggcg aactcagcga aagatcgtgc
     1801 cgaaaatctg atgattgtcg atttaatgcg taatgatatc ggtcgcgttg ccgtagccgg
     1861 ttcggtaaaa gtaccagagc tgttcgtggt cgaacccttc cctgccgtgc atcatctggt
     1921 cagcaccata acggcgcggc taccagaaca gttacacacc agcgatctgc tgcgcgcagc
     1981 ttttcctggt ggctcaataa ctggggctcc gaaagtacgg gctatggaaa ttatcgacga
     2041 actggaaccg caccgacgca atgcctggtg cggcagcatt ggctatttga gcttttgcgg
     2101 caacatggat accagcatta ctatccgcac gctaactgcc attaacggac aaatatactg
     2161 ctctgcgggc ggtggaattg tcgccgatag ccaggaagaa gcggaatatc aggaaacttt
     2221 tgataaagtt aataagatcc tacgtcaact ggagaaataa acgtggaaca ccgtagcctg
     2281 acgcttgatg attttttatc gcgctttcaa cttttgcgtc cgcaaatgaa ccgggaaacg
     2341 ttaaatcatc gtcaggctgc cgtgttaatc cccatcgtcc gtcgaccgca accggggttg
     2401 ttgctgactc agcgttcgat tcatctgcgt aaacacgctg gacaagtggc attccctgga
     2461 ggcgcagtcg atgacacgga cgcatcagtt atcgccgccg cgctgcgcga agctgaagaa
     2521 gaggtcgcta taccgccttc cgccgttgaa gttatcggcg tgctgccgcc cgtcgatagc
     2581 gtaactggct accaggtaac cccagtggtc ggcattatcc cgcccgatct gccgtatcgc
     2641 gccagtgaag atgaagtctc ggcggtgttt gaaatgccgc tcgcccaggc attacatctg
     2701 ggtcgttatc atcctttaga tatctaccgc cgtggcgatt cacatcgggt atggctgtcc
     2761 tggtacgaac agtattttgt atggggaatg accgcaggca taattcgtga gctggcgctg
     2821 caaattggtg tgaaaccctg actatactta tctttacatc tacaaaacac tacttgagac
     2881 aatcatcgca atattagtta aatcgcggtt tttgattagt ttaattcatg tgaatagtta
     2941 agccagtcgc cgcgttccct cttacactat gcgctgttat tagttcgtta ctggaagtcc
     3001 agtcaccttg tcaggagtat tatcgtgatt agtctattcg acatgtttaa ggtggggatt
     3061 ggtccctcat cttcccatac cgtagggcct atgaaggcgg gtaaacagtt cgtcgatgat
     3121 ctggtcgaaa aaggcttact ggatagcgtt actcgcgttg ccgtggacgt ttatggttca
     3181 ctgtcgctga cgggtaaagg ccaccacacc gatatcgcca ttattatggg tcttgcaggt
     3241 aacgaacctg ccaccgtgga tatcgacagt attcccggtt ttattcgcga cgtagaagag
     3301 cgcgaacgtc tgctgctggc acagggacgg catgaagtgg atttcccgcg cgacaacggg
     3361 atgcgttttc ataacggtaa ccttccgctg catgaaaacg gtatgcaaat ccacgcctat
     3421 aacggcgatg aagtcgtcta cagcaaaact tattattcca tcggcggcgg ttttatcgtc
     3481 gatgaagaac actttggtca ggatgctgcc aacgaagtga gcgtgccgta tccgttcaaa
     3541 tctgccaccg aactgctcgc gtactgtaat gaaaccggct attcgctttc tggtctcgct
     3601 atgcaaaacg aactggcgct gcacagcaag aaagagatcg acgagtattt cgcgcatgtc
     3661 tggcaaacca tgcaggcatg tatcgatcgc gggatgaata ccgaaggcgt actgccaggc
     3721 ccgttgcgcg tgccacgtcg tgcgtcggcc ctgcgccgga tgctggtttc cagcgataaa
     3781 ctgtctaacg atccgatgaa tgtcattgac tgggtaaaca tgtttgcgct ggcagttaac
     3841 gaagaaaacg ccgccggtgg ccgtgtggta actgcgccaa ctaacggtgc ctgcggtatc
     3901 gttccggcag tgctggctta ctatgaccac tttattgaat cagttagccc agacatctat
     3961 acccgttact ttatggcagc gggcgcgatt ggtgcactgt ataaaatgaa cgcgtctatt
     4021 tccggtgcgg aagtcggttg ccagggcgaa gtaggtgttg cctgttcaat ggctgctgcg
     4081 ggccttgccg agctgttggg cggtagcccg gaacaggttt gtgtggcagc ggaaattggc
     4141 atggagcaca acctcggtct gacctgcgac cccgttgcag ggcaagttca ggtgccgtgc
     4201 attgaacgta atgccattgc ctctgtgaag gcgattaacg ccgcacggat ggctctgcgc
     4261 cgcaccagtg caccgcgcgt ctcgctggat aaggtcatcg aaacgatgta cgaaaccggt
     4321 aaggacatga acgctaaata ccgcgaaacc tcacgcggtg gtctggcaat caaagtccag
     4381 tgtgactaat acttcttact cgcccatctg caacggatgg gcgaatttat tcccgctttc
     4441 tcgtctgctg taattttccc cactacactt ccactgttgc gtcaggcgtt tgtcgccata
     4501 cgcttacagg gtggcccgca tgcaaaaagc acaacggatc attaaaacct atcgccgtaa
     4561 tcgaatgatt gtttgtacga tttgcgcact cgttacgctc gcttcgaccc tgagcgtgcg
     4621 atttatttca cagcgtaact taaatcaaca acgggtagta caattcgcca atcacgctgt
     4681 agaggaatta gataaagtac tgcttcccct acaggcaggt agcgaagtct tgcttccgct
     4741 gattggtctg ccctgctctg tcgcccattt gccattacgt aaacaggcgg caaaactcca
     4801 aactgtgcga tccattggcc tggtgcaaga cggcacactt tattgctcca gcatttttgg
     4861 ttatcgcaat gtgcccgtcg tggacattct ggctgaactt cctgcaccgc aaccactttt
     4921 acgcctgacg accgaccatg ccctgattaa aggcagtccg gttttgattc aatggacgcc
     4981 agcagcgggc agtagcaaag ctggggtcat ggagatgatt aacatcgact tactggcggc
     5041 aatgctgctt gagccacaac tgccgcaaat cagtagcgcc agcctgacgg tggacgatcg
     5101 gcatttgctc tatggtaatg ggctggtaga ttcccttccg caacctgaaa acaatgaaaa
     5161 ctatcaggtt tcttcgcaac gctttccttt taccattaac gttaatggtc cgggggctac
     5221 ggcgctggca tggcactatc ttccaacaca attaccgctg gcggtgctgc taagtttact
     5281 tgtgggctat atcgcctggc tggcgaccgc ttaccggatg agtttttccc gcgaaatcaa
     5341 tctgggcctg gcgcaacatg agttcgaatt gttctgccag cctttgctta atgcgcgcag
     5401 ccagcaatgt attggcgtag agattttgct gcgctggaac aatccgcgtc agggctggat
     5461 ttcgccggat gtgtttattc ctatcgcgga agaacatcat ttaattgtgc cactgacccg
     5521 ctatgtgatg gcagaaacca ttcgtcagcg ccatgttttc ccgatgagta gtcagtttca
     5581 tgttggcatt aacgtcgcac ccagccattt tcgccgtggt gtgctgataa aagatctcaa
     5641 tcagtactgg tttagcgctc acccgattca gcaactgatc ctcgaaatca ccgaacgcga
     5701 tgccttactg gatgttgatt atcggattgc ccgcgagcta catcgtaaaa acgtcaaact
     5761 ggcgattgat gacttcggca ccggcaatag ttcattttcc tggcttgaaa cattacgtcc
     5821 tgacgtgctg aaaattgata agtcatttac cgcagctata ggttctgacg cggttaactc
     5881 gacggtgacc gatatcatca tcgcactggg gcaaagactg aatattgaac tggtggcgga
     5941 gggtgtggaa acgcaagaac aggcgaaata tttgctccgt catggcgtcc atattttgca
     6001 agggtatttg tacgcacagc cgatgccact acgtgatttt cccaaatggc tggcgggcag
     6061 ccaaccgccg cccgcccggc ataatggaca tatcacaccc attatgccgt tacgttaacg
     6121 cttactcatc ttcatcgtgc gccggttgct ctttaacaat gcgaaccaga tcaacacgat
     6181 aatcattggc ttcaatgatg gtgatatgca gtggccctac atcaatcaca tcgcccacac
     6241 ggggaatgtg accatttgcc gagatcacga ggcccgcgac cgtcgcgata tcatcgtcat
     6301 cggcaaggtg ctcaacatca agcgcctgct gcaaggcatg caaatctgta ccgcctttta
     6361 ccagccagcc gtcaccgtcg gtaatgattt ccggcgtttc gtcagcgtcc gggaattcac
     6421 ccgcaatggc ttccagcaca tccagcggcg tgaccaagcc ttgtaccaca ccaaactcgt
     6481 tggtcacgat aacaaagctc ccgcgagcac gacgcagcac gcccagcagg ttgatcggat
     6541 cgagggtttc cgggacgata atcgccggag acgccgaagc aatcgccgcc acatcaacgc
     6601 cctcttccag cgccaccagc agttctttag cacgtacgat tccgatgatt tcatccagtt
     6661 caccgcgaca taccgggaac aggctgtgcg gtgaggagag cagctgctcg cggatttcat
     6721 ctaccccgag attagcgtca acccagctta tttcaccgcg cggcgtcatg atcccgcgca
     6781 gagaacgcga cgccagcgtc agtacgccgt taatcatgta acgttcttct tcggcaaatg
     6841 caccttccgg gatcggcatc ggcatcgggt tatcggcatc gtgctgaaca ttggcctgac
     6901 gtttcccgcc catcaaacgc aggatggcat cggcagtacg cgctcgcagc ggcaaagtcg
     6961 actggtgacg aataaagttg cgacgcgcaa tctggttaaa cacttcgatg atgatcgaga
     7021 agccaatcgc ggcatacagg taacctttcg gaatgtggaa accgaaacct tctgccacca
     7081 gactcagacc aatcattaac aggaagctca gacagagcac caccaccgtg gggtgctggt
     7141 taacaaatcg cgtcagcggt ttcgatgcca gcagcataac tgccatcgca atcactaccg
     7201 ccgccatcat caccggcaaa tggttaacca tccctactgc agtaattacc gcatccaacg
     7261 agaagacggc gtcaaggatg acaatctgtg tgacgaccac ccagaaactg gcgtagcctt
     7321 taccgtggcc ggaatcatga tcgcggtttt ccagccgttc atgcagttcg gttgttgctt
     7381 tgaacagcaa gaatatgccc ccgaacaaca taatcaggtc gcgtccggag aaggagaaat
     7441 ccatgacggt aaatagcggt ttggtcagcg tgaccatcca tgaaatcagc gacagcagcc
     7501 ccagacgcat aatcagcgcc agtgataatc ccagcaaacg cgctttatcg cgttgttttg
     7561 gcggcagttt gtcagcaaga atggcaatga agaccaggtt atcgataccc agcacaattt
     7621 cgagaacaac aagcgtgagt agccccgccc aaattgaggg gtccattaag aattccatga
     7681 caagctcctg cttaaggaat agctattcga cgccagaaat aatgcaggcg taacgacaaa
     7741 atgcaaacga aaggtgcggc atagagtgcc agaaaggcag gcgttaaaag gcctgatgct
     7801 gaaatgacgt cggtgacgat ccatactgcg ggctactgcc ctatactcca tggttgttaa
     7861 acgggagtta aacatatcag agacgcctct gatttggcaa agatttacct tcctttgcaa
     7921 acgaatgtga caaggatatt ttacctttcg aaatttctgc taatcgaaag ttaaattacg
     7981 gatcttcatc acataaaata attttttcga tatctaaaat aaatcgcgaa acgcaggggt
     8041 ttttggttgt agcccttatc tgaatcgatt cgattgtgga cgacgattca aaaatacatc
     8101 tggcacgttg aggtgttaac gataataaag gaggtagcaa gtgaccattg ctattgttat
     8161 aggcacacat ggttgggctg cagagcagtt gcttaaaacg gcagaaatgc tgttaggcga
     8221 gcaggaaaac gtcggctgga tcgatttcgt tccaggtgaa aatgccgaaa cgctgattga
     8281 aaagtacaac gctcagttgg caaaactcga caccactaaa ggcgtgctgt ttctcgttga
     8341 tacatgggga ggcagcccgt tcaatgctgc cagccgcatt gtcgtcgaca aagagcatta
     8401 tgaagtcatt gcaggcgtta acattccaat gctcgtggaa acgttaatgg cccgtgatga
     8461 tgacccaagc tttgatgaac tggtggcgct ggcagtagaa acaggtcgtg aaggcgtgaa
     8521 agcactgaaa gccaaaccgg ttgaaaaagc cgcgccagca cccgctgccg cagcaccaaa
     8581 agcgactcca actccggcaa aaccaatggg accaaacgac tacatggtta ttggccttgc
     8641 gcgtatcgac gaccgtctga tacacggtca ggtcgccacc cgctggacca aagaaaccaa
     8701 tgtctcccgt attattgttg ttagtgatga agtggctgcg gataccgttc gtaagacact
     8761 gctcacccag gttgcacctc cgggcgtaac agcacacgta gttgatgttg ccaaaatgat
     8821 tcgcgtctac aacaacccga aatatgctgg cgaacgcgta atgctgttat ttaccaaccc
     8881 aacagatgta gagcgtctcg ttgaaggcgg cgtgaaaatc acctctgtta acgtcggtgg
     8941 tatggcattc cgtcagggta aaacccaggt gaataacgcg gtttcggttg atgaaaaaga
     9001 tatcgaggcg ttcaagaaac tgaatgcgcg cggtattgag ctggaagtcc gtaaggtttc
     9061 caccgatccg aaactgaaaa tgatggatct gatcagcaaa atcgataagt aacgtattgt
     9121 gttgattatc actcagtttt cacacttaag tcttacgtaa acaggagaag tacaatggag
     9181 attaccactc ttcaaattgt gctggtattt atcgtagcct gtatcgcagg tatgggatca
     9241 atcctcgatg aatttcagtt tcaccgtccg ctaatcgcgt gtaccctggt gggtatcgtt
     9301 cttggcgata tgaaaaccgg tattattatc ggtggtacgc tggaaatgat cgcgctgggc
     9361 tggatgaaca tcggtgctgc agttgcgcct gacgccgctc tggcttccat catttctact
     9421 attctggtta tcgcaggtca tcagagcatt ggtgcaggta tcgcactggc aatccctctg
     9481 gccgctgcgg gccaggtact gaccatcatc gttcgtacta ttaccgttgc cttccagcac
     9541 gctgcggata aggctgctga taacggcaac ctgacagcga tttcctggat ccacgtttct
     9601 tctctgttcc tgcaagcaat gcgtgtggct attccggccg tcatcgttgc gctgtctgtt
     9661 ggtaccagcg aagtccagaa catgctgaat gcgattccgg aagtggtgac caatggtctg
     9721 aatatcgccg gtggcatgat cgtggtggtt ggttatgcga tggttatcaa catgatgcgt
     9781 gctggctacc tgatgccgtt cttctacctc ggcttcgtaa ccgcagcatt caccaacttt
     9841 aacctggttg ctctgggtgt gattggtact gttatggcag tgctctacat ccaacttagc
     9901 ccgaaataca accgcgtagc cggtgcgcct gctcaggcag ctggtaacaa cgatctcgat
     9961 aacgaactgg actaacaggt gagcgaaatg gttgatacaa ctcaaactac caccgagaaa
    10021 aaactcactc aaagtgatat tcgtggcgtc ttcctgcgtt ctaacctctt ccagggttca
    10081 tggaacttcg aacgtatgca ggcactgggt ttctgcttct ctatggtacc ggcaattcgt
    10141 cgcctctacc ctgagaacaa cgaagctcgt aaacaagcta ttcgccgtca cctggagttc
    10201 tttaacaccc agccgttcgt ggctgcgccg attctcggcg taaccctggc gctggaagaa
    10261 cagcgtgcta atggcgcaga gatcgatgac ggtgctatca acggtatcaa agtcggtttg
    10321 atggggccgc tggctggtgt aggcgacccg atcttctggg gaaccgtacg tccggtattt
    10381 gcagcactgg gggccggtat cgcaatgagc ggcagcctgt taggtccgct gctgttcttc
    10441 atcttgttta acctggtgcg tctggcaacc cgttactacg gcgtagcgta tggttactcc
    10501 aaaggtatcg atatcgttaa agatatgggt ggtggcttcc tgcaaaaact gacggaaggg
    10561 gcgtctatcc tcggcctgtt tgtcatgggg gcattagtta acaagtggac gcatgtcaac
    10621 atcccgctgg ttgtctctcg cattactgac cagacgggca aagaacacgt taccactgtc
    10681 cagactattc ttgaccagtt aatgccaggc ctggtaccac tgctgctgac ctttgcttgt
    10741 atgtggctgt tgcgcaaaaa agttaacccg ctgtggatca tcgttggctt cttcgtcatc
    10801 ggtatcgctg gttacgcttg cggcctgctg ggactgtaag actgttgtac actaccgggg
    10861 ccttttggcc ccgttttttt atctggagga ttaatgacaa tcacggacct ggtactgatt
    10921 cttttcatcg ccgcactcct ggccttcgcg atctacgatc agttcatcat gccccgccgt
    10981 aacggcccca ccctgctggc aattcctttg ctccggcgtg gtcgcatcga tagcgttatc
    11041 ttcgtcggat tgattgtcat tcttatctat aacaacgtca cgaatcatgg cgcgctaata
    11101 acaacatggt tattaagtgc actggctctg atgggttttt atatattctg gatccgcgtt
    11161 ccgaagatca tctttaaaca aaaaggtttt ttcttcgcca atgtctggat tgaatatagc
    11221 cgaatcaaag cgatgaactt gtcggaagat ggcgtgttgg tgatgcaatt agaacagcgc
    11281 cggctgttaa tccgcgttcg aaatatcgac gatctggaaa aaatttataa gcttctcgtt
    11341 tcaactca
//
LOCUS       DAZKJR010000156        11341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKJR010000156 DAZKJR010000000
VERSION     DAZKJR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.157"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..727
                     /gene="gspC"
                     /locus_tag="ACHOWS_004321"
     CDS             2..727
                     /gene="gspC"
                     /locus_tag="ACHOWS_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFQ0049811.1"
                     /translation="MIFNVNYFHTTIVKNGQIINQPTNAFQSEFSLAALWRNENHAGV
                     KDANPVAVNQETPKLSIALNGIVLTSNDETSFVLINEGSEQKRYSLNEALESAPGTFI
                     RKINKTSVVFETHGHYEKVTLHPGLPDIIKQPDSESQNVLADYIIATPIRDGEQIYGL
                     RLNPRKGLNAFTTSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGV
                     PRLINVSVAELTGMNGQRHERTQ"
     gene            711..2663
                     /gene="gspD"
                     /locus_tag="ACHOWS_004322"
     CDS             711..2663
                     /gene="gspD"
                     /locus_tag="ACHOWS_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFQ0049812.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNERKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHTPAPSAR"
     gene            2673..4154
                     /gene="gspE"
                     /locus_tag="ACHOWS_004323"
     CDS             2673..4154
                     /gene="gspE"
                     /locus_tag="ACHOWS_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFQ0049813.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLENEA"
     gene            4151..5347
                     /gene="gspF"
                     /locus_tag="ACHOWS_004324"
     CDS             4151..5347
                     /gene="gspF"
                     /locus_tag="ACHOWS_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFQ0049814.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTAVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5357..5794
                     /gene="gspG"
                     /locus_tag="ACHOWS_004325"
     CDS             5357..5794
                     /gene="gspG"
                     /locus_tag="ACHOWS_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFQ0049815.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5802..6311
                     /gene="gspH"
                     /locus_tag="ACHOWS_004326"
     CDS             5802..6311
                     /gene="gspH"
                     /locus_tag="ACHOWS_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFQ0049816.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6308..6685
                     /gene="gspI"
                     /locus_tag="ACHOWS_004327"
     CDS             6308..6685
                     /gene="gspI"
                     /locus_tag="ACHOWS_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFQ0049817.1"
                     /translation="MNKQSGMTLLEVLLAMSIFATVALALMSSMQGQRNAIERMRNET
                     LALWIADNQLKSQDTFNDENTSSSGKEIINGEEWNWRSDVHSSKDGTLLERTITVTLP
                     NGQKTALTRYQSIHDRSGQAQDD"
     gene            6678..7265
                     /gene="gspJ"
                     /locus_tag="ACHOWS_004328"
     CDS             6678..7265
                     /gene="gspJ"
                     /locus_tag="ACHOWS_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFQ0049818.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLGVLAFMVFSQASTLHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPQAPLNEAQTL
                     QTVHWYLRNHTLYRAVRTSVDGRKNQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7258..8241
                     /gene="gspK"
                     /locus_tag="ACHOWS_004329"
     CDS             7258..8241
                     /gene="gspK"
                     /locus_tag="ACHOWS_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFQ0049819.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQFWPQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8256..9419
                     /gene="gspL"
                     /locus_tag="ACHOWS_004330"
     CDS             8256..9419
                     /gene="gspL"
                     /locus_tag="ACHOWS_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFQ0049820.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTPTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMRCYGVAPHGVAATNPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QHQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMQKTEKDPVSGVWTVRSSGK"
     gene            9416..9877
                     /gene="gspM"
                     /locus_tag="ACHOWS_004331"
     CDS             9416..9877
                     /gene="gspM"
                     /locus_tag="ACHOWS_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFQ0049821.1"
                     /translation="MIKSWWAEKSTSEKQLVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEQITLKHLSFREQP"
     gene            9877..10554
                     /gene="gspO"
                     /locus_tag="ACHOWS_004332"
     CDS             9877..10554
                     /gene="gspO"
                     /locus_tag="ACHOWS_004332"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFQ0049822.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10583..11059)
                     /gene="bfr"
                     /locus_tag="ACHOWS_004333"
     CDS             complement(10583..11059)
                     /gene="bfr"
                     /locus_tag="ACHOWS_004333"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFQ0049823.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREES"
     gene            complement(11131..11325)
                     /gene="bfd"
                     /locus_tag="ACHOWS_004334"
     CDS             complement(11131..11325)
                     /gene="bfd"
                     /locus_tag="ACHOWS_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFQ0049824.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
BASE COUNT         2953 a         2919 c         2722 g         2747 t
ORIGIN      
        1 gattattttt aatgtgaatt atttccatac aactatagtt aaaaatggac agatcattaa
       61 tcaaccaaca aacgcatttc agtctgagtt cagccttgcc gctttatggc gtaacgaaaa
      121 tcatgctggg gtaaaagatg caaatcccgt ggcagttaat caggaaacac ctaagctctc
      181 tatagcgctt aatggcattg tactcacatc gaatgatgag acttcttttg tcttaattaa
      241 tgaggggagc gaacaaaagc gatactccct caacgaagcc cttgagtcag cgccgggaac
      301 gtttattaga aaaataaata aaaccagcgt tgtgtttgaa actcacgggc attacgaaaa
      361 agtaactctc catccagggc tacctgacat catcaagcag cctgattcag aaagtcaaaa
      421 tgtactcgct gattatatta tcgccacgcc gatacgtgat ggagaacaga tatacgggct
      481 tcgattgaac cctcgtaagg gcctcaatgc atttaccacc agcttgctac aaccagggga
      541 tatcgccctc aggatcaata atctttcgct gacccaccct gatgaggttt cacaggcatt
      601 aagtttactg ctaactcaac aaagtgctca gtttacaatt cgtcgcaacg gcgtaccccg
      661 cttgataaat gtttccgtcg cggaacttac aggaatgaat ggacagcgtc atgaaaggac
      721 tcaataaaat cacctgctgc ttgctggcag cactactcat gccttgtgca gggcacgctg
      781 agaacgaaca atacggcgct aacttcaata acgccgatat ccgccagttc gtggaaatag
      841 tgggtcagca tcttggcaaa acgatcctga tcgacccttc ggtacaggga accatttccg
      901 tacgcagtaa tgatacgttt agccaacagg agtactacca gttcttttta agtattcttg
      961 atctttacgg ttattccgtg atcacgctgg acaatggttt tctgaaagtg gtgcgctcag
     1021 ctaatgtaaa aacatcgcca gggatgattg ctgacagttc tcgtccaggc gtaggtgatg
     1081 agttggtcac ccgaatcgta ccgcttgaga acgttcctgc tcgtgacctg gctcccctgc
     1141 tccgccagat gatggatgcg ggtagcgtcg gtaatgttgt gcattatgaa ccctccaacg
     1201 ttcttattct gaccggtcgt gcctccacca ttaataaact gattgaagtc ataaagcgcg
     1261 ttgatgtcat cggcacagag aagcagcaaa ttattcatct ggaatatgcg tcagcggaag
     1321 atctcgccga aattttgaat caattaatca gcgaaagcca cggtaaaagc cagatgcctg
     1381 ccctcctttc cgcgaagatt gtggcggata agcgaaccaa ctctcttatc atcagcgggc
     1441 cggaaaaagc acgccagcgc atcacttcat tactgaaaag ccttgatgta gaagagagcg
     1501 aggaaggaaa tactcgggtt tattacctga aatatgctaa agccacgaat ctggtggaag
     1561 tgctaaccgg tgtttccgaa aagctgaaag atgaaaaagg gaatgagcgt aagccctcct
     1621 cttctggcgc gatggataac gtcgccatta ccgccgatga acagactaac tctctggtca
     1681 ttaccgctga ccagtccgtc caggaaaaac tcgccacggt aattgcgcgt ctggacattc
     1741 gccgtgcaca ggtgctggtt gaggcaatca tcgttgaagt tcaggatgga aatggactta
     1801 acctcggcgt gcaatgggcg aataaaaacg ttggagcaca gcaatttacc aataccggat
     1861 taccgatttt taacgctgcg caaggtgtgg ctgattataa aaagaatggt gggatcacca
     1921 gcgcgaatcc tgcctgggat atgtttagcg cctacaatgg catggccgca ggctttttca
     1981 atggcgactg gggcgtattg ttgactgcgc tggccagtaa caataaaaat gacatcctcg
     2041 ccaccccgag catcgtaacg ctggataata aactcgcgtc cttcaacgtt ggtcaggatg
     2101 tgccggtgct atctggctct cagaccactt ctggggataa cgtctttaat actgtcgaac
     2161 gcaaaacggt gggcacaaaa ctcaaagtca ctccgcaggt caatgaaggc gacgcggtac
     2221 tgctcgaaat agagcaggag gtttccagcg ttgactcttc gtctaattcg acgctcggcc
     2281 cgacatttaa cacccgaact attcaaaatg ccgtgctggt caaaaccggt gaaacggtgg
     2341 tcctgggcgg attgctggat gatttttcta aagagcaagt gtcaaaggtt cctctgcttg
     2401 gcgatattcc tttagtgggg caactcttcc gctatacttc gaccgagcgc gctaaacgca
     2461 acctgatggt atttatccgc ccaacgatta tccgtgacga tgatgtttat cgctcactgt
     2521 caaaagagaa atacacccgc taccgtcagg agcaacaaca gcgaatcgac gggaaatcaa
     2581 aagcgctggt tggctcggaa gatttgccgg tgctggatga gaacacgttc aacagccata
     2641 cccctgcgcc ctcggcacgg taaggcattc ttatgagaat tcactcaccg taccccgcca
     2701 gttgggcgct ggcacaacga attggttatc tctattcaga gggcgagatt atttatctcg
     2761 ccgatacgcc attcgaacgg ttactcgata ttcaacgtca ggttggccag tgccagacca
     2821 tgaccagctt gtcacaggct gattttgaag cccggctgga agcggtgttc catcagaata
     2881 ccggtgagtc gcaacagatt gcgcaggata tcgatcaatc cgtcgatctt ctctcgcttt
     2941 cggaagagat gcccgcaaat gaagatctcc tgaatgaaga ttcagcggca ccggttatcc
     3001 gcttgatcaa tgcgattttg agtgaggcca tcaaagaaac cgcctccgat atccacattg
     3061 aaacctatga aaaaacaatg tcgatccgtt ttcgcatcga cggtgttttg cggacaattt
     3121 tacagccaaa caaaaaactg gcggcactgc ttatctcccg aattaaggtc atggctcgtc
     3181 ttgatatcgc cgaaaaacgt attccacagg atggacgtat tagtttgcgt atcgggcgac
     3241 gtaacataga tgtccgcgta tccacactgc cgtccatcta tggtgaacgc gccgtgctcc
     3301 gtctgctgga taaaaacagc ctccagcttt cattgaacaa cctggggatg acggcagcgg
     3361 ataaacagga tttagaaaat ctcatccagc ttccacacgg tattatcctg gtgaccggac
     3421 cgacaggctc cggtaaaagc accacgctct acgccatcct ttcggcgctg aatactcccg
     3481 gccgcaatat tctgacggta gaagatcccg tggaatatga gctggaaggc attgggcaaa
     3541 cgcaggtgaa tacccgtgtg gatatgtctt tcgctcgcgg cctgcgcgcc atacttcgcc
     3601 aggacccgga tgtcgtcatg gtgggggaaa ttcgtgatac agaaaccgcg cagattgcgg
     3661 ttcaggcctc gctcaccggc catctggtac tctcaacact ccacactaac agtgcatcag
     3721 gcgcagtgac ccggctccgc gacatgggcg tcgaatcatt cctgctttcg tcttccctgg
     3781 cagggattat cgcgcaacgt ctggttcgtc gcctgtgtcc gcaatgccga caattcacgc
     3841 ccgtatcacc ccaacaagcg cagatgttta aatatcatca gctcgcggtg acaacaattg
     3901 gcactcccgt aggctgccct cattgccatc aatccggcta tcaggggcgc atggcgatcc
     3961 acgaaatgat ggtggtgacg ccggaattac gggccgctat tcatgaaaat gtggatgaac
     4021 aagcactgga gcgactggtc cggcaacaac acaatgcctt aatcaaaaat ggcctgcaaa
     4081 aagtgataag cggtgacacc tcctgggatg aggttatgcg cgtcgccagt gccacgctgg
     4141 agaacgaagc atgaattatc gctatcgcgc catgacccag gatggtcaaa aattgcaggg
     4201 gatcattgat gctaacgatg aacgtcaggc acgactgcgg ctgcgtgaag aagggctttt
     4261 cctgctggat attcgccccc aaaaaagttc gggagtaaaa acacgtcgcc cgaggatcag
     4321 ccatagtgaa ctgacgcttt tcacccggca gttggcaacc ttaagcgcag cggcattacc
     4381 tctggaagag agccttgccg taatcggtca acaaagcagt aataaacgac tggctgatgt
     4441 gttaaatcag gtacgcagcg ccatccttga agggcatccc ctttccgatg cgttacaaca
     4501 ttttcccacg cttttcgatt cgctctaccg taccctggtt aaagcgggcg aaaagagcgg
     4561 gctgctggcc ccggtgttgg aaaaactggc tgattacaat gaaaatcggc agaaaatccg
     4621 cagcaaactc attcagtcgc tgatctaccc ctgcatgctg actgcggtgg cgattgtggt
     4681 cgtgattatt ctcctcactg ctgtcgtgcc caaaattacc gaacagttcg tgcatatgaa
     4741 gcagcaactg ccgctgagta cacgcattct tttaggtctg agcgacacgt tgcaacatac
     4801 cggcccgaca ttactagcga cagtgtttat tgtcgctgta ggtttctggc tctggttaaa
     4861 acgcggcaat aaccgccacc gttttcatgc catgttgctg cgcgttgcac tcatcggccc
     4921 gctgatttgc gccattaaca gcgcacgcta tctccgcact ttaagtattt tgcaatccag
     4981 cggcgtccct ctgctggatg ggatgaattt gtccaccgaa agtctcagca acctcgaaat
     5041 ccgcaagcgt ctggcaaatg cggcagaaaa cgttcggcag ggtaacagca ttcatctttc
     5101 gctggaacaa acggcaatct tcccgccgat gatgctctac atggtggcct ctggcgaaaa
     5161 aagcgggcag ctcggcacgt taatggtcag agcagcagat aaccaggaga cactccaaca
     5221 aaatcggatc gccttaacgc tctccatctt cgagccagca ctcattatta cgatggcact
     5281 gatcgtcctg tttattgtcg tctcggtact ccaacctctt cttcaactta actcaatgat
     5341 taattaagga aagtatatgc gcgcaacgga taagcaacgc ggttttacat tactggaaat
     5401 tatggtggtg atcgtcatca ttggcgtact tgccagcctg gtggttccca acctgatggg
     5461 caataaagaa aaggcggata aacaaaaagc cgtcagcgat attgtggcgc tggaaaacgc
     5521 cctggatatg tacaagctcg acaatcatcg ctatccaacc acaaatcagg gacttgaatc
     5581 cttagtcgaa gcgccgacac tgccaccgct ggccgcaaac tataacaagg aaggttatat
     5641 caagcgtctg cctgccgatc cctggggcaa tgattatgtc ctcgttaatc ctggtgaaca
     5701 tggtgcatac gatctgcttt cagcagggcc cgatggtgaa atgggaaccg aggacgacat
     5761 caccaactgg ggtttgagca agaagaaaaa gtaagtcagc gatgaatcag caacgcgggt
     5821 ttaccctgct ggagatgatg ctggtgctgg cgctggtcgc tatcacggca agtgtggtgc
     5881 tcttcacata tggtcgggag gatgccgcca acactcgtgc gcgcgaaaca gcggcgcggt
     5941 ttaccgctgc acttgaatta gccatcgacc gcgctacgct cagtggtcag cctgtcggta
     6001 tccatttctc tgattcagca tggcgcatta tggtaccggg caaaacacca tcagcctggc
     6061 gctgggttcc attacaagaa gatgccgcag acgagagcaa aaatgactgg gatgaagaac
     6121 tctcaattca tcttcagcca tttaaacctg atgacagcaa ccaaccgcag gtggtgattc
     6181 tggccgacgg acaaatcacg cccttctctt tgttgatggc taacgcaggg accggtgagc
     6241 cgctcctgac gttagtctgt tcaggatcat ggcccctgga tcaaacgctc gccagagata
     6301 cccgcccatg aacaaacaat cagggatgac actgcttgaa gtcttactgg cgatgagtat
     6361 tttcgccact gttgcgctgg ctttaatgag cagcatgcaa gggcaacgaa atgccatcga
     6421 gcggatgcgt aatgagacgc tggcgctgtg gatcgcagat aaccagctta agtcgcaaga
     6481 tacattcaac gatgaaaaca cgtccagttc aggcaaagag ataattaatg gcgaagagtg
     6541 gaactggcgc agcgatgtcc attcaagcaa agacggcacg cttctggaac gcaccataac
     6601 ggtgacgtta cccaacggtc agaaaaccgc tctgactcgt tatcagagca tccatgatag
     6661 atccgggcag gcgcaagatg attaatcgcc aacagggttt cacattgctg gaagtcatgg
     6721 cggcactggc gatcttctca atgcttggcg tgctggcgtt tatggttttt tcgcaagcgt
     6781 ctacgctaca tcaacgctca cagaaagaaa tccagcagtt caatcaactg caacgcacca
     6841 tcaccattct tgataacgat ctcctgcaac tggtcgctcg ccgaaatcgg agtacggata
     6901 aaatcatggt gttgggtgaa gaggctattt ttaccacgca aagtcgcgat ccgcaggccc
     6961 cgctcaatga agcgcaaacc ttacaaacgg ttcactggta tctgcgaaat catacgcttt
     7021 accgtgctgt tcgtacttct gtggatggca ggaagaatca acctgcccag gcgatgctgg
     7081 agcatgtcga gagctttctt ctggaaagta acagcgggga aagccaggag cttcctctct
     7141 cagtcacgtt acatctgaag actcagcaat atggggcgtt acagcgccgt tttgcattac
     7201 ctgagcagtt agcgagagaa gagtcgccag ctcagacgca ggccgggaat aataaccatg
     7261 aataatgaac aacgcggcgt agcactgctc attgtactca tgttactggc gctcatggct
     7321 gctctggctg cagacatgac gatcagtttt cacggccagc ttcatcgaac ccgccaggta
     7381 aaccatcatt tgcaacggca atatgatatt gagctggcgg agaaacttgc gcttgccagc
     7441 ctcaccctgg acgttaaaga taacgacaga caaaccacgt tgcagcagtt ctggccacaa
     7501 ccgcaacagt tgcagctgga aaatggcaat acagtgaagt ggcagttacg cgatgcccag
     7561 cactgtttta atctcaatgc cttagcaaaa atctctgacg ccccgctggc ttcccctgat
     7621 ttccccgccc aggtattcag tgccctgctg attaacgccg gtatcgatcg cggaaacacc
     7681 gatgagatcg tgcaatcaat tgctgactat attgatgtcg atgactcacc acgctttcac
     7741 ggcgcagaag ataactttta ccaaagccag acgccgcccc ggcatagtgc caatcagatg
     7801 ctttttctga ccggcgaatt gcgtcagata aaaggcataa ccgaaaacat ctatcagcgg
     7861 cttatcccgt atgtctgcgt tctcccaacc acggaactta gcatcaatct taatatgctt
     7921 acggagaacg atattccgct cttcagagcg ttattcctca ataacatcac ggatgctgac
     7981 gctcgggtct tattacaaaa aaggcccagg gaaggctggc taaccacgga tgcatttctt
     8041 tactgggcgc agcaggattt ctccggcgtg aagcctctgg tagctcaggt gaaagggcat
     8101 ctctttccct atagccgcta ttttacgctg agtacggaaa gtatcagcga cgaacaatcc
     8161 cagggatggc aaagccatat tttcttcaac cgtaaacagc agagcgccca aatctatcgg
     8221 cgtacgctgc aactctacta atcataagga tcgtgatgcc tgaatcgttg atggtcatac
     8281 gttcttcctc gacgctgcgt aagcattggg aatggatgac gttctccgca gatagcgtat
     8341 cttcggtaca tacgcccacg gacgatcttc cgctggagtc gctggccgat cagcccggtg
     8401 ccggaaatgt tcatcttttg atcccaccag agggcctttt atatcgttcc ctcacgctgc
     8461 ccaacgccaa atacaaactc accgcacaaa cattgcaatg gctggcagag gagacgttac
     8521 ccgacaacac ccaggactgg cactggacgg ttgtcgataa acaaaacgaa agtgtcgagg
     8581 tgataggcat tcagtcggag aaactcagtc gctatcttga gcgtttacat actgccggac
     8641 tcaacgtgac gcgggttttg ccagatggct gttatcttcc ctgggaagta gatagctgga
     8701 ctctggttaa ccagcaaaca agctggctta ttcgcagtgc cgcacatgcc ttcaatgaac
     8761 tggatgaaca ctggttacag catcttgctg cccagtttcc gcctgagaat atgcgttgtt
     8821 atggtgtggc acctcacggc gttgcagcga ccaatccact tatccagcat cctgaaatac
     8881 catcactgag tttgtattct gccgatattg cattccagcg ctatgacatg ctgcatggtg
     8941 tttttcgtaa acagaaaact gtaagcaaat cgggaaaatg gctcgcccgc ctggcagtaa
     9001 gctgtctcgt tctcgccatt ctttcttttg ttggcagccg gggcatcgcg ctctggcaaa
     9061 cgcttaagat tgaagatcaa cttcagcatc aacagcaaga aacctggcaa cgttatttcc
     9121 cgcagatcaa acgcacccac aactttcgtt tttactttaa gcagcaactc gctcagcaat
     9181 atcctgaagc tgtgccgttg ctctatcatt tacaaacact tctgctggaa caccctgaac
     9241 tgcagttaat ggaggcgaac tatagccaga agcaaaagtc gctgacctta aaaatgagtg
     9301 ctaagagtga agccaatatc gatcgtttct gtgaattaac acagtcctgg cttccgatgc
     9361 aaaaaacaga aaaagatccg gtcagtggtg tatggacagt aaggagctca gggaaatgat
     9421 taaatcatgg tgggcagaaa aatccacatc ggaaaaacag ctcgttgcgg cgctggcggt
     9481 tctctcgctc ggcgtttttt gttggcttgg ggtgataaaa ccgatagata cctacattgc
     9541 agagcatcag tcccacgcgc aaaaaattaa gaaggacatc aaatggatgc aggatcaagc
     9601 gagcacccat ggtttactcg gtcatcctgc gcttacgcag cccatcaaaa atatccttct
     9661 cgaggaagca aaacgtgaga atttggctat cacgctggaa aacggtccgg acaatactct
     9721 gacaattcat cctgtcacag ctcccctcga aaacgtctcc cgctggctca ctacggcaca
     9781 gattacatac ggtattgtta ttgaagacct tcagtttact cttgccggaa acgaacagat
     9841 caccctcaag catttgtcct tcagggagca accataatga caatgctact gccgctcttc
     9901 attctggttg gttttattgc aggttatttt gttaatgcca tggcctatca cctctcgccg
     9961 ttggaggata aaacggcgtt aacgtttcgc caggtattgg ttcatttcag gcaaaaaaaa
    10021 tatgcctggc atgatacagt gcccctgata ctttgcgttg ctgctgctat cgcctgcgcc
    10081 ctggcacctt tcacgcccat cgtgactggc gcactctttc tctatttctg tttcgcactc
    10141 acgctcagtg ttattgattt tcgcactcag ctcctgcccg acaaactcac cttaccgctg
    10201 ctctggcttg gcttggtatt taatgcgcag tctggattaa ttgatttaca tgatgcggtt
    10261 tacggcgcgg tagctggcta tggggtgctg tggtgtgttt actggggcgt ctggttagtt
    10321 tgtcacaaag agggattggg ctacggtgat ttcaagctac tggccgctgc aggcgcgtgg
    10381 tgtggctggc aaacgttgcc aatgatactg ctaattgcct cactgggtgg cattggttac
    10441 gccatcgttt cacaacttct gcaacgccgg actataacca ctatcgcatt cggcccgtgg
    10501 cttgcgctcg gcagcatgat aaacctgggg tatctggcct ggatctcgta ttaaccggga
    10561 gggttctccc tcccgacacg gctcaacttt cttcgcggat ctgtgcttgc agataatttt
    10621 gcaggcccat cttctgaatc agatcaagtt ccgtttccag ccagtcgata tggccttctt
    10681 catcacgcaa aatttctatc atcatatcgc ggctgacgta atcatgaacg ctatcggcat
    10741 aaccaattgc ctcacgcaaa ttcttcgcgc catccagctc aagtgccaga tcagaacgca
    10801 gcatttcctc aacatcctca ccaatgttca gtttgcccag gtcctgtaaa tttggaagac
    10861 cttccagaaa aagaatgcgc tcaatataac gatcggcgtg tttcatctca tcaatggatt
    10921 catgatactc cacatcattg agacgtttga gaccccagtt tttaaacatc cgggcatgga
    10981 gaaagtactg attgattgcg acaagctcat ttcccaacag tttgttgaga taatttataa
    11041 ccttagtatc acctttcatt ttttgactcc ttcgcttcca ctctttgaag cgtagaacgg
    11101 cttacgagta agtcaaaaaa cacactccgt ttatgcggac tccttaaact ccggcaactg
    11161 cattaattca tcctccatca cttcacgcgc ggcccggaca catttaccgc actgattgcc
    11221 gaccggaata aattttttta attgctggaa cgagtgaggg gaaaactggc gcacagcctg
    11281 acgaattttt ttatcgctga taccattaca aagacaaacg tacatagaga ctcccgtata
    11341 c
//
LOCUS       DAZKJR010000157        11322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKJR010000157 DAZKJR010000000
VERSION     DAZKJR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.158"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            207..1421
                     /gene="manD"
                     /locus_tag="ACHOWS_004335"
     CDS             207..1421
                     /gene="manD"
                     /locus_tag="ACHOWS_004335"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFQ0049825.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTASGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1488..2771
                     /locus_tag="ACHOWS_004336"
     CDS             1488..2771
                     /locus_tag="ACHOWS_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0049826.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3023..3940
                     /gene="oxyR"
                     /locus_tag="ACHOWS_004337"
     CDS             3023..3940
                     /gene="oxyR"
                     /locus_tag="ACHOWS_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFQ0049827.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3923..5323)
                     /gene="sthA"
                     /locus_tag="ACHOWS_004338"
     CDS             complement(3923..5323)
                     /gene="sthA"
                     /locus_tag="ACHOWS_004338"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFQ0049828.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5655..6305
                     /gene="fabR"
                     /locus_tag="ACHOWS_004339"
     CDS             5655..6305
                     /gene="fabR"
                     /locus_tag="ACHOWS_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFQ0049829.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6305..6664
                     /gene="yijD"
                     /locus_tag="ACHOWS_004340"
     CDS             6305..6664
                     /gene="yijD"
                     /locus_tag="ACHOWS_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFQ0049830.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLL
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6704..7804)
                     /gene="trmA"
                     /locus_tag="ACHOWS_004341"
     CDS             complement(6704..7804)
                     /gene="trmA"
                     /locus_tag="ACHOWS_004341"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFQ0049831.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7918..8108
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8173..10056
                     /gene="btuB"
                     /locus_tag="ACHOWS_004342"
     CDS             8173..10056
                     /gene="btuB"
                     /locus_tag="ACHOWS_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFQ0049832.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            10001..10858
                     /gene="murI"
                     /locus_tag="ACHOWS_004343"
     CDS             10001..10858
                     /gene="murI"
                     /locus_tag="ACHOWS_004343"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0049833.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            11231..>11322
                     /locus_tag="ACHOWS_004344"
     rRNA            11231..>11322
                     /locus_tag="ACHOWS_004344"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2680 a         2626 c         2989 g         3027 t
ORIGIN      
        1 atggagtttg ttatgtgatt atttaacgtg ttgttttaac gatgatagct aattattcag
       61 gtggtttttg agggtgtttt ttctcctcgg tagaaatttt tatttttaat gtgatcatgg
      121 ttgaataatc gctctttctc tcttgtatgg tagtaggcgc aaaattaagt ttgaaacgtc
      181 tatttacaac acaggagatt gcaataatga aaattattgc tgcagatgtt tttgttactt
      241 gcccagggcg taatttcgtt acgcttaaaa taaccactgc aagcggactt tgtggattag
      301 gagatgccac gttaaatggt cgcgaattgt cggtcgcttc ttatctgaaa gatcatcttt
      361 gtccacaact aattggccgc gatgccagcc gtatcgaaga tatctggcaa ttcttttata
      421 aaggtgctta ctggcgacgt ggccccgtca cgatgtcagc catttctgcc attgatatgg
      481 cgttgtggga tatcaaagca aaagcggcaa atatgccatt gtatcaactg cttggtggtg
      541 cttctcgtga aggcgttatg gtttattgcc acaccaccgg gcgtacgatt gatgaagtcc
      601 ttgaagacta tgcaaagcac cagcagatgg gctttaaggc gattcgtgtg cagtgtggtg
      661 taccgggtat gcaaaccacc tatggtctgg cgaagggcaa aggactggcg tatgaaccag
      721 caaccaaagg cctgtggcca gaagagcagt tgtggtcttc agaaaagtat ctcgatttta
      781 cgccgaagtt atttgaagcg gtgcgtaata agttcggttt taacgaacat ctgttgcatg
      841 acatgcacca tcgcctgacc cccatcgaag cggcgcgctt tgggaagagc attgaagatt
      901 accgcctgtt ctggatggaa gatccgacgc ctgccgagaa tcaggaatgt tttcgtctga
      961 ttcgtcagca taccgtgacg ccgattgcgg tcggtgaagt gtttaacagt atctgggact
     1021 gcaagcagtt aatcgaagag cagctgattg actatattcg cacgacgatt acccatgcag
     1081 gcggcattac cgggatgcga cggattgccg attttgcatc gctttatcag gtacgtaccg
     1141 gttcgcatgg cccttcagat ttatcgcctg tttgccatgc ggcggcgctg cattttgacc
     1201 tctgggtgcc aaactttggc gttcaggagt acatgggcta ttccgaacaa atgcttgaag
     1261 tcttcccgca tagctggcgc ttcgatgaag gttatatgca cccaggcgat gaaccggggc
     1321 tgggtatttc tttcgatgag aaactggcgg cgaaatatcc gtatgacccg gcgtatctcc
     1381 ccgtcgcacg actggaagat ggcacattgt ggaactggta atagcggttc gcttaattat
     1441 tttctgaata ttcatcaaat cgattaattc cgctcaagga gctaaaaatg gacgttgatg
     1501 tgtcaaccag tgttgcgggt aataaaccgc aacggattcg tcgtattcag acagtgaccc
     1561 tggttttatt atttatggcg gggatcgtta attttctcga ccgttcgtca ttgagcgtgg
     1621 caggtgaagc aattcgtggc gagttaggat tatccgctac cgagtttggt gttttgcttt
     1681 ctgcattttc tctgtcttat ggtttttcac aacttccttc cggtattttg ctagaccgct
     1741 ttggcccacg aatagtgtta ggcgcaggct taatattctg gtcattaatg caggcattaa
     1801 caggaatggt taattctttt agccacttta ttttaatgcg tatcggtctg ggtattggcg
     1861 aagcgccatt tatgcctgcg ggagtaaagt cgatcaccga ctggtatgcg caaaaagaac
     1921 gcggcacggc gctggggatt tttaactcat ctaccgttat cggtcaggct atcgcgcctc
     1981 ctgctcttgt attgatgcaa ctggcatggg gctggcggac gatgtttgtc atcatcggcg
     2041 tagcagggat tctggttggg atctgttggt acgcgtggta tcgcaaccgg gcgcagtttg
     2101 tcctgaccga ggaagaacgg acatatctct ccgcgccggt taaaccgcgt ccacagttac
     2161 aatttagcga gtggctggcg ctgtttaagc atcggacaac ctgggggatg attttaggtt
     2221 tctctggcgt caattatacc ggttggctct acatcgcgtg gttacccggt tatttgcagg
     2281 ccgaacaagg tttcagtctg gcgaaaaccg gttgggtggc ggcaattcct ttcctcgcgg
     2341 cggcggtcgg gatgtgggtt aacggtattg ttgtcgatcg gctggcgaaa aaaggctatg
     2401 acctggcgaa gacgcgtaaa acggcgattg tctgcggttt gatgatgtcg gcattaggca
     2461 cgttgctggt cgtgcaatct tcctcgccag cccaggcggt agcgtttatc tcaatggcgc
     2521 tgttctgcgt gcatttcgcg ggaacatctg catgggggct ggtgcaggtg atggtgtcag
     2581 aaacaaaagt ggcttccatc gccgggattc aaaactttgg cagttttgtt tttgcttcct
     2641 ttgctccgat cgtaaccggt tgggtcgtgg ataccacaca ctcgtttaat ctggcgctgg
     2701 ttatcgcggc ctgtgtgacg ttcaccgggg cgctgtgtta cttctttatt gtcaaagatc
     2761 ggattgagta accgttacta tcaggctctc ttgctgtggg cctgtagaat aaaaaaaagc
     2821 ggatcctgga gatccgcaaa agttcacgtt ggctttagtt attcgagttg agaaactctc
     2881 gaaacgggca gtgacttcaa gggttaaaag aggtgccgct ccgtttctgt gagcaattat
     2941 cagtcagaat gcttgatagg gataatcgtt cgttgctatg ctacctatcg ccgcgaacta
     3001 tcgtggcaat ggaggatgga taatgaatat tcgtgatctt gagtacctgg tggcattggc
     3061 cgaacaccgc cattttcggc gtgcggcaga ttcctgccac gttagccagc cgacgcttag
     3121 cgggcaaatt cgtaagctgg aagatgagtt gggtgtgatg ttgctggagc ggaccagccg
     3181 taaagtgtta ttcacccagg cgggaatgct gctggtggat caggcgcgta ccgtgctgcg
     3241 tgaggtgaaa gtccttaaag agatggcaag ccagcagggc gagacgatgt ccggaccgct
     3301 gcatattggt ctgattccca cggttggacc gtacctgcta ccgcatatta tcccgatgct
     3361 gcaccagacc tttccaaagc tggaaatgta tctgcatgaa gcacagaccc accagttact
     3421 ggcgcaactg gacagcggca aactcgattg cgtgatcctc gcgctggtga aagagagcga
     3481 agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct atgaagacca
     3541 tccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg gcaggggaaa aactgctgat
     3601 gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt ttctgttttg aagccggggc
     3661 ggatgaagat acacacttcc gcgcgaccag cctggaaact ctgcgcaaca tggtggcggc
     3721 aggtagcggg atcactttac tgcctgcgct ggctgtgccg ccggaacgca aacgtgatgg
     3781 ggttgtttat ctgccatgca ttaagccgga accacgccgc actattggcc tggtttatcg
     3841 tcctggctca ccactgcgca gccgctatga gcagctggca gaggccatcc gcgcaagaat
     3901 ggatggccat ttcgataaag tgttaaaaca ggcggtttaa gccatttagc gcagctaccc
     3961 gataggcttc cgccatcgtc gggtagttaa aggtggtgtt gacgaagtac tcaatagtgt
     4021 tgccgccacc tttctgttcc ataatcgcct gaccgatatg aataatttcg gcagcgcgct
     4081 cgccaaagca gtgaataccc agaatctctt ttgtttcccg atggaacaaa attttcagcg
     4141 tgcccacgtt catgccgacg atttgtgcac gtgccagatg tttaaactgg gcgcggccca
     4201 cttcatatgg cactttcatc gcggtcagct gctgttcggt tttgcccaca gagctgattt
     4261 ccgggatggt gtaaataccg gtagggatat cttcaatcag atgtgcggtg gcttcacctt
     4321 tcaccagcgc ctgcgcggca atgcgcccct ggtcataggc cgccgacgcc aggctcggat
     4381 aaccaatcac gtcgcccacc gcgtaaacgt gcggctgtgc ggtctgatac atgctgttga
     4441 ccttcagctg tccgcggctg tcagtttcca gcccaatgtt ctgtaacgcc agcgaatcgg
     4501 tattaccggt gcgaccgttg gcatagagca ggcagtcagc tttcagtttt ttacccgact
     4561 tcaaatgcat gatcacaccg tcgtcacagc cttcgatctt ctcgtactct tcgttgtgac
     4621 gaatcactac gccactgttc cagaagtgat aggagagaga atctgacatc tcttgatcga
     4681 gaaatgccag caggcgatcg cgggtgttga tcagatccac ttttacatcc ataccgcgga
     4741 agatcgacgc atattcacag ccgatcactc cagcaccata gataagtaca tggcgcggtt
     4801 cgtggtgcat gctaagaatt gagtcgctgt cgtaaatgcg tggatgcgtg aaatcaacat
     4861 ctgttggatg atatggacga gagccgcaag caataacaaa tttttcagcg gttagtgtct
     4921 cgacgctgcc gtccgggcaa tccagcgcca gcgtgtgctc gtcaacaaag cgagcgttcc
     4981 cctgcaatat ttcacagtga ttacgttcgt aaaatccctg acgcatgcgc gtttgttgat
     5041 taatcacgtt atcggcatgg ttaaggatat cggcaaaaga agagcggagc agtcgcgaat
     5101 ggtcgctgta aagtgggttt tgattaaatt ctataatgcg gctgacggcg tgacggagag
     5161 ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc aacattttga taacgctcga
     5221 taactgcgac gcgcgcacct tgcttaacca ggcccattgc agcgccttcg ccgccggggc
     5281 cggaacctat tactatggca tcgtaatcgt aggaatgtgg catggtaggg cttacctgtt
     5341 cttatacata aaagcaacag aatggtaaca ttttatcgcg ggtaagccaa ttgatccccg
     5401 tcatttatct ggctatatcg tgagcggcct ttgctttgtc tgtttcttac ttttgacctg
     5461 aacgttttat tggattttta tcgacgatac tctccgttta agcggcaggt ttccgttgta
     5521 cgtaaaagaa ccggccaaag aattgcagta aatatgtttt attgcgttac cgttcattca
     5581 caatactgga gcaatccagt atgttcattc tctggtatag tgccagcagt acttttggca
     5641 aggattcaga catcgtgatg ggcgtaagag cgcaacaaaa agaaaaaacc cgccgttcgc
     5701 tggtggaagc cgcatttagc caattaagtg ctgaacgcag cttcgccagc ctgagtttgc
     5761 gtgaagtggc gcgtgaagcg ggcattgctc ccacctcttt ttatcggcat ttccgcgacg
     5821 tagacgaact gggtctgacc atggttgatg agagcggttt aatgctacgc caactcatgc
     5881 gtcaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat ccgcacctcg gtctccacat
     5941 ttatggagtt catcggtaat aatcctaacg ccttccggtt attattgcgg gaacgctccg
     6001 gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat tcagcacttc attgcggaac
     6061 ttgcggacta tctggaactc gaaaaccata tgccgcgtgc gtttactgaa gcgcaagccg
     6121 aagcaatggt gacgattgtc ttcagtgcgg gggccgaggc gttggatgtc ggcgtcgaac
     6181 aacgtcggca attagaagag cgactggtac tgcaactgcg aatgatttct aaaggggctt
     6241 attactggta tcgccgtgaa caagagaaaa ccgcaattat tccgggaaat gtgaaggacg
     6301 agtaatgaaa caagcaaatc aagatagagg tacgctgctg ctggcgttgg ttgctggctt
     6361 atcgattaat ggtactttcg cagcgctgtt tagctccatt gtgccatttt ctgtattccc
     6421 gattatttcc ctgttgctga cggtttactg cctgcatcaa cgttatctta atcgcaccat
     6481 gccggtaggc ttgccgggtc tggcagctgc ctgttttatt ctcggcgtac tgctgtacag
     6541 cacggtagtt cgtgcggaat atccggatat cggctctaac ttcttcccgg cagtactctc
     6601 cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac cgtaagcagg aagttgctga
     6661 gtaatcggta tcatgccgga tgctggtgca tccggcatgg gttttacttc gcggtcagta
     6721 atacgccgca ctccatatgg tgcgtgtagg ggaactgatc aaacagagcc agacgttcga
     6781 ccttgtgcgt ctggcttaat gtttccagat tcttgcataa cgtttccgga ttgcaggaga
     6841 tatacagaat acgtggatac gcctgcacca ttttctcggt ttcactgtcc agaccgctgc
     6901 gcggtgggtc gacaaaaatg gtttcgcact gataactctt taagtcgatt ccttgcaggc
     6961 ggttaaactc gcgtacgcca ttcatcgcct gagtaaattc ttctgccgcc atacgaataa
     7021 tttgtacgtt atcaatatgg ttagcggcaa tgttgtattg cgcggcagcg accgacggct
     7081 tagcgatttc ggtggctaat acccgatcaa aattgcgcgc cagtgctaat gaaaagttac
     7141 cgttgccgca gtacagctcc agcaaatcgc cttttgagcc tttggttacg tccagcgccc
     7201 attccagcat ctgaatattc atcgccgcgt tcggctgggt aaagctgttt tctacctgac
     7261 ggtagatcat ctctttccct gcgaccggca gacgttcatc gatgtaatcc tggtccagct
     7321 cgattttggt tttcgttgcc cgaccaatca gatgcacatt cagattctgc gctcgcagtg
     7381 catcgcgcag ggcttccgct tcctgacgcc actcatcatc cagcttctta tggtacagca
     7441 gggaaaccac cgcctgatta ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc
     7501 gcagaacggg attattacgc acacccgcaa tcatcgccgt catcaactgg ttgataagtt
     7561 cactggcggc ggggaagcta tccacgcgga tgcggctttt ggtttgttga tcgaaaatga
     7621 tgtgatacag gtcatcgcca tcgtgccaga tgcggaactc cgcacgcatc cggtaatgac
     7681 tgaccggcga gcgaaacact tccggaacca ggtcagaaaa cggtgccatc atactttgca
     7741 aacgtaccac tttttcggct aactgcgctt catactgttc tgttggaagg tgttcggggg
     7801 tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt agggattgct catcagatgt
     7861 ccagatcttg atgaattcct atttgtgagc tacgtctgga cagtaacttg ttacaacctg
     7921 tagcatccac ttgccggtcc tgtgagttaa tagggaatcc agtgcgaatc tggagctgac
     7981 gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg acactgccat ccggtgggaa
     8041 gtcatcatct cttagtatct tagatacccc tccaagcccg aagacctgcc ggctaacgtc
     8101 gcatctggtt ctcatcatcg cgtaatattg atgaaacctg cggcatcctt cttctattgt
     8161 ggatgcttta caatgattaa aaaagcttcg ctgctgacgg cgtgttctgt cacagccttt
     8221 tccgcttggg cacaggatac cagcccggat actctcgttg ttactgctaa ccgttttcag
     8281 cagccagtaa atactgtcct tgcacctact gatattgtta cccgtgagga tattcaacgc
     8341 tggcagtcga aagacctcaa cgatgttatg agtcatctcc ccggtgtaaa tatctctcaa
     8401 actggtggaa tggggacgag ttcttctctt tatgttcgag gcactgaatc gcgccatgtt
     8461 ttagtgttga ttgacggtgt gccgatggcc cgtgccggta tcgacaatgc aattgatatt
     8521 agccagttcc cggtatcact tgtacagcgt gttgaatata ttcgtgggcc gcgttctgcc
     8581 gtttatggtt ctggtgccat cggaggtgtg gtgaacatta ttacaatgag taatgatgag
     8641 aaagctcaga tcaacgcagg aatgggtaca aatggttatc aagcctatga tggggtaata
     8701 aataaacgtt ttggcgatac gatggtaaca gcagcaggtg cttaccagac gaccaaaggt
     8761 tttaacattc agcctggctc accatacagt ggtgatagcg atcgcgatgg atatcgcaac
     8821 aaacttttct ggggggggct gcagcataag tttaacgata acttctcggg gttcttccgt
     8881 ggttacagct acacagctaa taatgattac gaccagggct cgatgtatgg ttctgctact
     8941 ggcaacgaag aagctcagaa ttacaaccag tcctgggata ctggcctacg ctataactcc
     9001 ggcatctatt cgtctcagtt aattgctaac tatcaacgac tcaagaatta taactacaca
     9061 aataatcttg gtcgttatgc gggagatgcc accctggatg acatggaaca gcgttatatt
     9121 cagtggggta atagcattga ggtagggcat ggtgcaatta gcggcgggat cgattggaaa
     9181 caggaaaaac tgacgtccag cagcattaca aaatcagatg attacgaacg cgataccacc
     9241 gggttatacc tgactggaca gcaacagatt tccaatgtga ctttggaagc ttctggccga
     9301 gaagatcatg atgaacagtt tggctggcat ggtacctggc agacagctgc tggctgggag
     9361 tttattgatg gctaccaggc aacactttct tacggtacca gttttctttc gccttctctt
     9421 ggacagcaat acggggctgc tcgctttgct tcaatttatg gtcctggtat tgcagccaac
     9481 ccaaatttga agccagaaga atctaaacag tgggagattg gaattgaggg ggtaactggg
     9541 actctggatt ggcggctctc aggatatcgt tatgaaattc agaacctgat tgattataaa
     9601 agcataaata atgtcaacca gtacattaat gtgaaatcag ccacgattaa agggctggaa
     9661 tggacgggaa atattacaac cggtcctgtt gaacatcgct tgacgttaca atatgttgac
     9721 ccgcgcgatg atgaaacaga taaagtgctg taccgtcggg caaaacagca ggttaagtac
     9781 gagttgactg gacaagctta tggtttcgaa tgggacgtat cttaccaata tataggtcaa
     9841 cgatacgaca acgcttatga cgagacctct ggctcatcac atacagttaa attaggcggt
     9901 gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcatctaac agttcgtggt
     9961 aaaatagcca acctgttcga caaagattat gagacagtct atggctacca aactgcagga
    10021 cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg tgctggtgtt
    10081 tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct taccggatct
    10141 ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa gcgaagagtt
    10201 tattgttgag cgagtggtgg caattgtcac cgcagtgcaa gaacgttatc cccttgcgct
    10261 ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac gcgaaaagtt
    10321 cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc tgacggcaaa
    10381 tggcattgtc ggattactgg caacccgcgg aactgttaaa cgttcttata ctcatgagct
    10441 gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag agatggtcga
    10501 gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcgctaa aacgtatctt
    10561 gcgtccgtgg ttaagaatga aagagccgcc agataccgtt gtactggggt gtacccattt
    10621 ccctctacta caagaagaac tgttacaggt gctgccagag ggaacccggc tggtggattc
    10681 tggcgcagcg attgctcgcc gaacggcctg gctgttagaa catgaagccc cggatgcaaa
    10741 atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg aacaattatt
    10801 gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt taggctgatt
    10861 tggttgaatg ttgcgcggtc agaaaattat tttaaatttc ctcttgtcag gccggaataa
    10921 ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgcc gggtcagcgg
    10981 ggttctcctg agaatctcaa cagagaaaag caaaaaaatg cttgactctg tagcgggaaa
    11041 gcgtattatg cacaccccgc gccgctgaga aaaagcgaag cggcactgct ctttaacaat
    11101 ttatcagaca atctgtgtgg gcactcgaag atacggattc ttaacgtcgc aagacgaaaa
    11161 atgaatacca agtctcaaga gtgaacacgt aattcattac gaagtttaat tctttgagca
    11221 tcaaactttt aaattgaaga gtttgatcat ggctcagatt gaacgctggc ggcaggccta
    11281 acacatgcaa gtcgaacggt aacaggaaga agcttgcttc tt
//
LOCUS       DAZKJR010000158        11060 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKJR010000158 DAZKJR010000000
VERSION     DAZKJR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.159"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..198
                     /locus_tag="ACHOWS_004345"
     CDS             94..198
                     /locus_tag="ACHOWS_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049834.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            456..2654
                     /gene="speF"
                     /locus_tag="ACHOWS_004346"
     CDS             456..2654
                     /gene="speF"
                     /locus_tag="ACHOWS_004346"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFQ0049835.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEFAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2651..3970
                     /gene="potE"
                     /locus_tag="ACHOWS_004347"
     CDS             2651..3970
                     /gene="potE"
                     /locus_tag="ACHOWS_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFQ0049836.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4027..5667)
                     /gene="pgm"
                     /locus_tag="ACHOWS_004348"
     CDS             complement(4027..5667)
                     /gene="pgm"
                     /locus_tag="ACHOWS_004348"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFQ0049837.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5693..6238)
                     /gene="seqA"
                     /locus_tag="ACHOWS_004349"
     CDS             complement(5693..6238)
                     /gene="seqA"
                     /locus_tag="ACHOWS_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFQ0049838.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6423..7187
                     /gene="ybfF"
                     /locus_tag="ACHOWS_004350"
     CDS             6423..7187
                     /gene="ybfF"
                     /locus_tag="ACHOWS_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0049839.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7327..7620
                     /gene="ybfE"
                     /locus_tag="ACHOWS_004351"
     CDS             7327..7620
                     /gene="ybfE"
                     /locus_tag="ACHOWS_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFQ0049840.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7760..8290
                     /gene="fldA"
                     /locus_tag="ACHOWS_004352"
     CDS             7760..8290
                     /gene="fldA"
                     /locus_tag="ACHOWS_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFQ0049841.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8500..8586
                     /gene="uof"
                     /locus_tag="ACHOWS_004353"
     CDS             8500..8586
                     /gene="uof"
                     /locus_tag="ACHOWS_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFQ0049842.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            8579..9025
                     /gene="fur"
                     /locus_tag="ACHOWS_004354"
     CDS             8579..9025
                     /gene="fur"
                     /locus_tag="ACHOWS_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFQ0049843.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9109..9435)
                     /gene="chiQ"
                     /locus_tag="ACHOWS_004355"
     CDS             complement(9109..9435)
                     /gene="chiQ"
                     /locus_tag="ACHOWS_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFQ0049844.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9485..10891)
                     /gene="chiP"
                     /locus_tag="ACHOWS_004356"
     CDS             complement(9485..10891)
                     /gene="chiP"
                     /locus_tag="ACHOWS_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFQ0049845.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDIYDGTAWLQALTFGYRAADVVDLRLEGSWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2686 a         2782 c         2731 g         2861 t
ORIGIN      
        1 attgatcatt ttttattgat caccttcaca gttcaaccgt atttcctgga tagaatacct
       61 tcaccttcat tcacatcagg aaaggtaaat taaatggaaa ataacagccg cactatgccc
      121 catataaggc ggacaactca tattatgaag tttgctcatc gcaatagctt cgactttcac
      181 ttcttcaatg cccgttagtc taccgactaa gggcacttca gcgtacaggt cttcctgact
      241 ctctgtatta caggcatatg cctgtattcc gcagtgctca ttctctgtta acccttgtat
      301 tatcagccac cggactgata agggtttctg cattctgaaa aaagcaaact tctgattaat
      361 ttatcagcgg ggtaactttg ctttttttcc ggcacgatcg atttctcatt cgagaaattg
      421 aggacctgct attacccaaa ataaagagat ggaaaatgtc aaaattaaaa attgcggtta
      481 gtgattcttg cccggactgt tttaccacgc agcgagaatg tatctatatt aatgaaaaca
      541 gtaatatcga tgtggcggca atagttttat cgctcaacga tgttacacgc ggaaaactcg
      601 atgaaatcga tgccacgggt tatggcatcc cggtatttat tgctactgaa aatcaagaac
      661 gtgtacccgc agagtatttg ccccgtattt cgggtgtctt tgagaattgc gaatcgcgac
      721 gagaatttta tggtcgtcag ttagaaaccg cagccagcca ttatgaaacg caactgcgcc
      781 cgcccttctt ccgcgcactg gtcgattatg tcaatcaggg taacagcgcg tttgattgcc
      841 ccggtcatca gggcggcgaa tttttccgtc gccatcccgc ggggaatcag tttgtggaat
      901 actttggtga ggcgctgttc cgtgctgact tgtgcaacgc cgacgtagcg atgggcgatc
      961 tgctgattca cgaaggcgcg ccatgcattg cacagcaaca tgcggcaaaa gtgtttaatg
     1021 ccgataaaac atacttcgtt ttaaatggca cttcatcttc taacaaagtg gttttaaacg
     1081 ccctgctgac accgggtgat ctggtgctgt ttgaccgcaa taaccacaaa tctaaccacc
     1141 acggagcgtt gctacaggct ggcgcgacac cggtttatct ggaaaccgca cgtaacccgt
     1201 atggttttat cggtggtatt gacgctcact gttttgaaga aagttacctg cgtgaactga
     1261 tcgcggaatt cgcaccgcag cgggcaaaag aggctcgtcc tttccgcctc gccgttattc
     1321 agctaggcac ttacgacggt acgatttata acgcccgtca ggtggtggat aaaattggtc
     1381 atctgtgtga ctacatcctc tttgactccg catgggtcgg ctatgagcag tttatcccga
     1441 tgatggcgga ctgttcacca ctgttgctgg atcttaatga gaacgatccg ggtattctgg
     1501 ttacgcaatc tgtgcataaa cagcaggctg gtttttccca gacttcacaa atccataaaa
     1561 aagacagcca tatcaaaggg caacagcgtt atgtaccgca caaacgcatg aacaacgcct
     1621 ttatgatgca tgcctccacc agcccgttct atccgctgtt tgccgcactg gatatcaacg
     1681 ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg gtaaatggca
     1741 ttaatgcccg caaactaatc ctcgataact gtcagcatat tcgtccattc gtacctgaac
     1801 tggtggatgg taaaccctgg cagtcgtatg aaacggcgca aattgcggtt gatctgcgct
     1861 tcttccagtt tgtaccaggt gagcactggc attcttttga aggctatgca gagaatcaat
     1921 actttgtcga tccgtgcaaa ctgttgctga caaccccagg tattgatgca cgtaacggcg
     1981 aatatgaagc gttcggtgta cccgcgacga ttcttgctaa cttcctgcgc gaaaatggcg
     2041 tagtgccgga aaaatgcgat cttaactcca tcctctttct gctgactccg gcagaagata
     2101 tggccaaact tcagcaactt gttgctctgc tggtacgctt cgaaaaactg cttgagtccg
     2161 acgcgccatt atcagaagta ctgccttcca tctacaaaca gcatgaagag cgctacaccg
     2221 gttataccct gcgtcagttg tgtcaggaaa tgcatgattt gtatgcccgt cacaacgtga
     2281 aacaactgca aaaagagatg ttccgtaagg aacacttccc acgcgtaagc atgaatccac
     2341 aagaagccaa ctacgcctat ttacgcggtg aagtggagct ggttcgtctg ccggatgcag
     2401 aaggccgtat cgctgccgaa ggtgcgcttc cttatcctcc gggtgtgctg tgtgttgttc
     2461 cgggtgaaat ctggggtggt gctgttctgc gttacttcag cgctctggaa gaagggatca
     2521 acctgctgcc aggttttgca ccagagctgc agggtgtcta tatcgaagaa catgatggtc
     2581 gtaagcaagt ttggtgctat gtcatcaagc ctcgtgatgc gcaaagcacc ctgttgaaag
     2641 gggaaaaatt atgagtcagg ctaaatcgaa taagatgggc gtcgttcagt taaccatact
     2701 gacgatggtc aacatgatgg gctccggtat catcatgctg ccgacaaagc ttgccgaagt
     2761 tgggacaatc tcaattatct cctggctggt gacagccgta ggctcaatgg cactggcatg
     2821 ggcattcgca aaatgcggta tgttcagccg taaatcaggc ggtatgggcg gttatgcgga
     2881 gtatgctttc ggtaaatccg ggaactttat ggcgaactac acctacggtg tctcgctgct
     2941 gattgctaac gtcgcgattg ctatttcggc ggttggttac ggcaccgaac tgctcggcgc
     3001 gagtttgtcg ccagtgcaga ttggtcttgc gaccatcgga gtgctgtgga tttgtaccgt
     3061 ggctaacttt ggtggtgcgc gcattaccgg gcaaatcagt agcattactg tgtggggggt
     3121 cattattccg gtcgtcggtc tgtgcattat cggctggttc tggtttagcc cgacgctata
     3181 cgctaactca tggaacccgc accatgtgcc gttctttagc gctgtaggtt cttccatcgc
     3241 catgacgttg tgggctttcc tcggtctgga gtcagcctgt gccaacgccg aagtagttga
     3301 aaacccggaa cgtaacgtgc cgattgcagt tcttggcggt acgttaggtg cggcggtgat
     3361 ttatatcgtc tccaccaacg tgattgccgg gattgtgcca aatatggagc tggcaaattc
     3421 aacggcacca tttggtctgg ccttcgcgca gatgttcacg ccggaagtgg gtaaagtcat
     3481 tatggcgctg atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc
     3541 ccaggtgttt aaatcttctg ccgatgaagg gtacttcccg aaaattttct cccgtgtgag
     3601 caaagtcgat gcaccagtac agggaatgct gaccattgtc atcatccaga gcggattatc
     3661 actgatgacc attagcccgt cgctgaacag tcagttcaac gtgctggtta acctggccgt
     3721 ggtgaccaat atcattccgt atattctgtc aatggcggca ttagtcatta ttcagaaggt
     3781 cgctaatgtg cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat
     3841 gtacagcttt tatgcgctgt actcatccgg ggaagaagcc atgctatacg gttccatcgt
     3901 gaccttcctc ggttggacac tgtatggtct ggtctcacca cgctttgaac tgaaaaataa
     3961 acacggttaa ttaagcaaag caaaaaaggg cgatcttccg accgcccttt ttcttatttg
     4021 atatgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt tctcaatctg
     4081 cttgcgatgt tcttcaccaa ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc
     4141 tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc cgatagaagc
     4201 gccattaccc ggtgccgccg tcagacgcgc ggtgatcggg tcacctgcca gggtgctggc
     4261 gctcaccatt tccggagaca gcttagacaa cgccgctttt tgtgcggaag tcgctgctgc
     4321 ctgcaaacgg ttatagctcg gcgcaccaaa gcgttttgcc agttcgttat agtgttcctg
     4381 cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa tgataccgtc
     4441 tttatcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcgcctg cactctcttc
     4501 gccgccaaag ccgaagctgc catcgaacaa accatcaaca aaccatttga aacctaccgg
     4561 tacttctacc agcttacggc ccaaatcatt gaccacacgg tcgatcatcg cagatgaaac
     4621 cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga acaggtagtt
     4681 gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga cgataccgtg
     4741 acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca gtgccagcag
     4801 gcccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt tatcgaggtg
     4861 cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga ggttgtaata
     4921 ctcgccaatg cgcttccagt attcgatacc ggaaccaccc agcggatcaa cgcccagcgt
     4981 caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt ccacaaacgg
     5041 ctgcaccaga tcctgctctt tcacatgacc ggatgccatc gcttcgtcga gggagatacg
     5101 cttcacgcct ttcaggccat cagccagcag tgcgttggct ctgtcttcca ccactttagt
     5161 gacgttggta tcagccgggc caccatttgg cggattgtac ttgataccac catcttccgg
     5221 cgggttatgg gacggtgtaa tcacgatacc gtctgccagt gggccacctt ttttattgtg
     5281 aaccagaatg gcattggaaa tggcaggtgt cggagtgaag ccattgtttt cctgcacaat
     5341 gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag gctcggacag
     5401 ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg cacgttcttc
     5461 agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc gcgctgcact
     5521 gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg cttctggttt
     5581 cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct gttgtgcagg
     5641 ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg ttttagatag
     5701 ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg atgtgttcga
     5761 tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc gtaccaggca
     5821 catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt
     5881 aaacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag
     5941 aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc tctgcgtatt
     6001 cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt ttgaccggct
     6061 tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct
     6121 gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat
     6181 gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc gttttcatct
     6241 taatccagtg cagtgtctta gctgaataca agtcgccagg agtatatgaa taaatcccgg
     6301 cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta tttgttgcag
     6361 ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa aagaactttg
     6421 ccatgaaatt gaatatccgc gcgcaaactg cacaaaacca gcacaataat tctcccatcg
     6481 ttcttgtcca tggtctgttt ggcagcctcg acaaccttgg cgtactggct cgcgatctgg
     6541 taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca ccgagagatc
     6601 cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat gcacagcaga
     6661 tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg gctcttactg
     6721 cactagcccc cgatcgcatt gacaagctgg tggcgattga tattgcgccg gtcgattatc
     6781 acgttcgccg tcatgatgag attttcgcgg ccatcaacgc agtcagtgaa tcagacgcac
     6841 aaacacgcca gcaagcagcg gcaataatgc gccagcatct taatgaagaa ggggtgattc
     6901 agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtgcca gtattgtggg
     6961 atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcat cctaccctgt
     7021 ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat ttactggcac
     7081 aatttccaca ggctcgggcg catgtgattg caggcgcggg tcactgggtc catgctgaaa
     7141 aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc aactgattaa
     7201 aaacccagcg tccgcgtcgc tttccgcgcg tggacgttgg cgtgactcat cgcgctgatg
     7261 tattatggcg cgctatccat ccgggcaggc gcctggctga ttgtttcccc cgaagtcacc
     7321 aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc gcacgagcgt
     7381 cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg agcagctgcg tattaataaa
     7441 cgcaaccaac taaaacgcga caaagtacgt ggccttaagc gtgtcgaact gaagctgaac
     7501 gcggaagctg tcgaggcact gaacgagctg gcggaatcgc gcaatatgag ccgtagcgag
     7561 ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa
     7621 atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg
     7681 cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt tcaataagtt
     7741 tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg acaccggtaa
     7801 taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg ccgatgtcca
     7861 tgacattgca aaaagcagca aagaagatct ggaagcatat gacattctgc tgctgggcat
     7921 cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc cgactctcga
     7981 agagattgat ttcaatggta aactggttgc gctgtttggt tgtggcgacc aggaagatta
     8041 cgcagaatac ttctgcgatg cattaggcac catccgcgac atcattgaac cgcgcggcgc
     8101 aactatcgtt ggtcactggc caaccgcggg ctaccacttc gaagcgtcaa aaggtctggc
     8161 tgatgatgac cactttgtcg gtctggctat cgacgaagat cgtcagccgg aactgactgc
     8221 tgaacgtgta gaaaaatggg ttaaacaaat ttctgaagag ctgcatctcg acgaaattct
     8281 caatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag ataatgccaa
     8341 tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat gtcccggtgt
     8401 tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac aatatttgcc
     8461 agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat tatctcaaga
     8521 gcaaattctg tcacttctcc taatgaagtg aaccgcttag taacaggaca gattccgcat
     8581 gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc ctcgtttaaa
     8641 aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag atttatacaa
     8701 acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg tactgaacca
     8761 gtttgacgat gctggtatcg tcacccgcca caattttgaa ggcggtaaat ccgtatttga
     8821 actgacacag caacatcacc acgatcacct gatctgcctc gactgcggca aggttatcga
     8881 atttagtgat gattccatcg aagcgcgtca gcgtgaaatc gccgcaaaac atggcattcg
     8941 cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt gccgcgaaga
     9001 tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac ctgcaggttg
     9061 gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt atttgttatt
     9121 actgcgaatt tcctgccaga ccttatcaca atcggctttc accgcctgat catttccggt
     9181 ttgcgttgcc tgaatacact gctgataatc cagtacgcgg acactttcct gctcggcaaa
     9241 ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg cttcaatttt
     9301 ttccggcgaa ccttgcgcgg tgttgataca ggcgctgtaa gcctctttca gacgactgtc
     9361 ttcctgcggt gcggttgatt gcgcacaagc taccagcccc gatgccatca tggcgatgag
     9421 aatcagtttt ttcatagcaa attcctcaaa tgtgcggcag gcacaaaacc tgccgcgtcg
     9481 ggcatcagaa gatggtgaat ggtgcgatta ccataaattt cacgtcgcgc tcatcctgga
     9541 agatgttacc gtaaccgccg ccccagcttg ggatgtcgga gtggttgtcg tattcggtga
     9601 agtgcagttt gaacatcgtg cctttggcgc gaccgtcctg aatggtgtac accgcatcca
     9661 ggctgtaggc agactcttca atagtccggt ttttgtcgta gtacgcatcc ggattgctct
     9721 gccaggtcgc aggtttagca tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg
     9781 gaagattcca gtttttcagg tcatacatcg caccgaagaa gaccgctttt tcgccgttgg
     9841 cgttgaagtc agaacggtta tcccaccaga tatccaggcg accgttcgag gaagcgtagg
     9901 ttggagtcat acgttgcagg aagtatccct gctgaccgtc ggctttaacc cagctgcctt
     9961 ccagacgcaa atccactaca tcagccgccc ggtaaccaaa ggtcaacgct tgcagccagg
    10021 cagtgccgtc gtaaatgtcg ttgacgctgc gatcgtcaac tttatcgcgc gtaccgtaga
    10081 actggtagct ggtggttaac gggctaccgg cgatatcaaa tttgtagctg gctttggcaa
    10141 aatattgatc tatataccct tccgcctgac caaatgccgc ttccagtaca aagttatttt
    10201 tgaagtcgta cttcgcaccg atggagtgca gataatcaac tttggtagtt ttatcgttct
    10261 gataaaactc atccatttcg agatgccacg gtgctttgta ttcgttggtc cacatgtagg
    10321 agaaactcaa cgcaccagca tcgccgtaat caaaattcgc cccggcttcc gcaccctgat
    10381 aagtacccgg cataaagctc cagtgcggcg ctaacagcgt ctgaccagtt ggctgaatgt
    10441 aacctgccct cgcccacacc ggaccgtatt taaatttggc cgctgcttta tacaggctta
    10501 taccgctttt gtcgccggac cagtcttcgt cataggcttt attgcttttt gaaaacgcga
    10561 tttcgttcgg gtgagagctg tcaccgtttt ccgccatttc aatcgccgta aacgcagcaa
    10621 tatcaaggcc gaacatatca gcagcataac cggactgaaa atcgaggttg gcattccagg
    10681 tggagtgaga aaggttggtt ttgtatttgt cgccgtcggt aacatcttta cggtcgcgtt
    10741 cacgctgcca gtaatagata ccgccggtta aggttgaatc gtcgatgaat ccttcggcgc
    10801 gagcctccgg cattgccata aagcccgaca ttgctgtaac accggcgata gccagcgcca
    10861 gcgtactacg tttgccacta aacgtacgca tgggttaatc ctctttgacg tataaattgc
    10921 tgcaccaaag gtgaacagcg aaaaaaataa aaattaaaaa agtagcgttg agaaaagtct
    10981 cgctgactac aggaacagac tatttttcgc gacgcgaatt atcaatctct ttctgtaacc
    11041 agaatgtaag aacatgagca
//
LOCUS       DAZKJR010000159        11047 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKJR010000159 DAZKJR010000000
VERSION     DAZKJR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.160"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            642..2198
                     /locus_tag="ACHOWS_004357"
     CDS             642..2198
                     /locus_tag="ACHOWS_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFQ0049846.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            2248..2967
                     /locus_tag="ACHOWS_004358"
     CDS             2248..2967
                     /locus_tag="ACHOWS_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFQ0049847.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            2978..4393
                     /locus_tag="ACHOWS_004359"
     CDS             2978..4393
                     /locus_tag="ACHOWS_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFQ0049848.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            4398..5093
                     /locus_tag="ACHOWS_004360"
     CDS             4398..5093
                     /locus_tag="ACHOWS_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFQ0049849.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
     gene            complement(5273..8389)
                     /locus_tag="ACHOWS_004361"
     CDS             complement(5273..8389)
                     /locus_tag="ACHOWS_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFQ0049850.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPVFPENASGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAAN
                     KSANYKPLRIATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQ
                     MNFSTDSKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPAPASIESEKEKKDFVKLFGEYLRAENVLQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDW
                     QHREKEADKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKSKDEMTVEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQREQQREADALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGHL"
     gene            complement(8509..9716)
                     /locus_tag="ACHOWS_004362"
                     /pseudo
     CDS             complement(8509..9716)
                     /locus_tag="ACHOWS_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(9713..>11047)
                     /locus_tag="ACHOWS_004363"
     CDS             complement(9713..>11047)
                     /locus_tag="ACHOWS_004363"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFQ0049851.1"
                     /translation="DAIRTKGYFIYPSQLFCNVAAKANTNDRLNADLNSIFVAIESSA
                     YGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVEGLNLGDFNKHQIDLFG
                     DAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHVNKIYDPAAGSGSLLLQ
                     AKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIKLGNTLTEPHFRDEK
                     PFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKADFAFVLHALNYLSARGR
                     AAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFGTTIAVNILVLSKHKTD
                     TRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHLAKSVAFETVVANDYNL
                     SVSSYVEAKDTREIIDIAELNAELKTTVSKIDQLRKDIDAIVAEIEGCEVQK"
BASE COUNT         2853 a         2460 c         2546 g         3188 t
ORIGIN      
        1 cgcgctgaaa aaagaaaacg tgggagaatt cgctctccgg ggcgtctgcg tccccggcgg
       61 agaataaacc ggagatgatg attctccaca ggagtattca atggcatacc attccccgga
      121 tgtcacactg cattatccgt atctgcgccg gatgtcctga cacgtctgcc tgaatggccg
      181 gaagagcgct tgcacgactt actaccgttc tcagcctacc gattcgcaga attaactgac
      241 gttacggtca taccacctaa tgcagggaaa cgagtcatgg aggtcgtcag ctctactccc
      301 atgaatgaca cagcctctga agtaaaaata ccgacgcagt gaaaccagcc ttaagctttc
      361 caaaacacaa ttacaacata gttgaacaaa gttcattcaa acaggttctt ttaataacat
      421 ttagtcaaat aaaataacaa taaaacgatt cagcaatcat ttttcataac aaaaacaaat
      481 tataaccccc ttccctgttt gtggacgaga ctcgttctct ccctgcgaga caagcgtaaa
      541 ttggtcacac cgacggtagt tgcctgaaaa ttacaaattc tcgtctgaca gggcaataac
      601 aacagtaatg atgctgaatt tgttttcgtt gaggaaatga catgcaccaa tacctgcttt
      661 ttttactggg acttacccca cttctggcaa tgatcatttt gattctgaaa gtcaaaatgc
      721 ccatacatta tgccgtactc agcacactct ttattacact gattcttgca actttgttct
      781 ggcacacacc agttcaggat ctgggttctg ctgttgtata tggcgcaatc aaagggctgt
      841 ggccgatagt gatagtcata cttgctgcca tttacagcta taacctgatg aatgcgacaa
      901 aaagcatgaa cattctgcga aatgttctgg ccgctgtcag tgatgataaa cgtatccagg
      961 tgcttcttat ctcatggtgt tttggtggat ttcttgaggc ggcggccggg tacggtacag
     1021 ctgtagcaat ccccattggt atactcattg cactgggctt taaccctctt aaagccgcaa
     1081 ttgcatcatt ggttgctaat acagtaccta ccgcgtttgg tgctgtcggc atacctgtat
     1141 cagtacttgc tgaacaggta aatttacccg taacaaccct cagcgggacg attattcttc
     1201 agctggcagc gttcaacatt ctgcttccct tcgttatagt cgctattatt ggggaaggcc
     1261 tgaaggctat acgaggcgtt gggttcatga ccttaatgtg cggtgtgagc acattaatcc
     1321 cacagtacct ggtggctgtt tatcttggcg cagaacttcc tgctttcgcg ggaagtctgg
     1381 tcagcctgat cgtgatagtc tttatggcaa aaaaccgtaa agaaaaaaca gctcctgaat
     1441 ttcttattga gacttcagca gatagcgcca gcgcactttc atactctcgt ggggcgttgt
     1501 tacgtgcgtg ctcaatctac atcctgattt ttctgttcat tcttctttgc tctccactgt
     1561 tcccgacggt aaaaagtaca gtgtctcaga ttgcctcgtc actcagcttc tctctgagta
     1621 acggcagcaa tctgtcgatg aagattgaat ggatcgcaac ccccggtgtt ctgattatca
     1681 ttgccagtgt tattggcggc ttgatacaga aagcatccct gtcatgcctg ttaaatgtat
     1741 ttgtcagtac ggtaaaacag ctcaaaaact ccattattgc catttctgcg attgttgcta
     1801 tggcaacggt aatggatgtt tgtgggttga ttggcactct ggcacagagc cttgtagatc
     1861 tcactggtgg ggcctacctc ttcattgctc cggttatcgg cgctcttggt acatttgtta
     1921 ctggcagtga cacaaactcc aacattctgt ttggtaaatt gcagaccact gcggctgaaa
     1981 aattgcatgt ggacccactc tggctggctg ccgctaacac atctggcgca acaggcggca
     2041 aaatgatttc tcctcagagt attgctatcg ctgtttcagc cacccgtatg gatggtcagg
     2101 ggagcgtaat catgtcaggg acactgaagt actgctttgc ttatattgtt attcttggac
     2161 tgaaagtagg ccttgtctac cacctgttca tggcctgaga aaaaataaca aacggacgtg
     2221 gaatattaat caacaggaga aatatctgtg aatgttaact ttttcgtcac ctgcattggt
     2281 gatgcattta aatcccgtat ggcacaaaac agcgtgctgt tactggagca attgggttgc
     2341 aatgtccatt tccccgaaaa acagggatgt tgcggtcagc cagcgataaa tggcggttat
     2401 gttcgtgatg ccattcctgg tatgaaaaat ctcatatctt cgctggaaga gaatgactgg
     2461 ccggtagtat ctcctgccgg gtcatgcact tacacgataa aaaattacgc tcgctatctc
     2521 agtgaagaac ctgagtgggc ggcacgagct gaaaaagtag ctgaacgcct gcatgatttg
     2581 acgtcattta tagtaaatca tcttggcatc acagatgttg gggcaagact caatggtcgc
     2641 gcagtgtacc atccttcatg cagcctttcc cgtaaactgg gtgtaacccg agaaccgctt
     2701 ttgttgcttg agaatgtacg gggacttgag ctgctgccgt ttcacgctca ggaaacgtgc
     2761 tgcgggtttg gcggcacgtt ctctgtaaaa atggcggaga tctctggtga aatggtgaaa
     2821 gagaaagtcg ggcatattat ggatgttaaa cctgattttc ttatcggggc tgacgccagt
     2881 tgcctgctca acatcggcgg tcggcttagc cgtgaagggc atcctgtaaa agtgatgcat
     2941 attgctgaag tcctgatgca cagatgaggt aaacagaatg tctttgaaaa caagtgatat
     3001 caggtttaaa gagcgtatcc gtattcagat tcaggacccg atcatgcgaa aagctgtggc
     3061 taacgcacag gagcgcatag ggactaatcg acagaaaatg gttgatgaac tggggcactg
     3121 ggaagaatgg cgttcacttg ctgaacagat tcgtcggcat gttctggaaa atcttgatgc
     3181 ttatttgtat caattgtccg aaaaagttac tgcgaatggc ggacatgtat attttgcccg
     3241 taccagagag gaggcaacca gttacatact gaaggtggcg aaagaaaaaa acgccaggaa
     3301 ggttgttaaa tccaaatcaa tggtgactga ggaaattggt atgaacggcg ttcttcagaa
     3361 agccgggata ccagtgatag aaacagatct tggtgaatat attcttcagc tggcgcatga
     3421 gccgccatcg catgttgttg tgccggcaat acataaagat cgccatcaaa ttcgcagagt
     3481 tttgcatgaa catctggggt atgacggccc tgaaactcca gaagcaatga cattgttcat
     3541 ccgtgagaaa atcagacagg attttctgag tgctgaagtt ggcgtaacag ggtgtaactt
     3601 tgccgttgca gaaacaggct cagtttgcct tgtgacaaac gaaggtaacg cacgtatgtg
     3661 caccacgtta cctaaaacac atattgctgt gatgggtatg gagcgtattg caccaacatt
     3721 ccgggaggtg gatgttctta tcaccatgct tgcaaggagt gctgtgggag caagactgac
     3781 aggttataac acctggctta caggtccaag agaagaagga catgttgacg gcccggaaga
     3841 gttccatctc gtcattgttg acaatgggcg ttcagaggtt ctcggctcag aattcagcga
     3901 tgtattgcgc tgtattcgct gcggtgcctg tattaatacc tgtcccgctt atcgccagat
     3961 tggtggacat ggctatggct caatctaccc gggcccaatc ggagcagtta tttccccgtt
     4021 gcttggtgga tatgaagact ttaaggaatt accttatgcc tgctcattgt gcactgcctg
     4081 caatggggta tgtccggtaa aaatacctct ggcacaacta atacaaaaac atcggcaggt
     4141 tatggcagag tccgaaataa caccatcaac agaacgccgt ataaccagaa tattcaatta
     4201 cgtaaacagt catccggcat tgtggaaagt tggcatgatt gctggcgcga aaacagccag
     4261 cctgtgcatc aggaacggaa aaacgccgat tagcccaggt gctattaaag aatggtctgt
     4321 ggccagagac ttaccttctc ctgatggaga aagcttccgt agttggttca aaagacataa
     4381 aaaaggaggg taactttatg gaaaacagag aggaatttct cgttgctatc gccagaaagc
     4441 ttggcaggcc agtaagacgt attcccgaag caatgccgga gcctgtaagt acgctggcta
     4501 cgacacgttt aaccgagttg acaatggacc aacgctgtga ggcttttatt aaatttgcct
     4561 cagaagtgat gcttgcgaag tgcgttctgg tttcgcctga agacgcacca tcaaaagcat
     4621 tagacatctg ttggacgttc ggttccgggc cagtcattat cagcaacgat caacgactgt
     4681 ttgatacggg gattgcacca ttgctacaaa aagagatggg cgctgtcctg tggaccccgg
     4741 agaagggtga ggaaaatatt catcttgcag aaaaagcaaa aacaggcgtc gttttcgctg
     4801 agtatggact gacagaatct ggtggtgttg ttttattctc cgcccctgaa cgagggcgtt
     4861 cagtaagtct tttacctgaa gcgtctgtgt ttgttttacg caaaagtacc attcttccca
     4921 gagtggctca gcttgcgcaa aaacttcatg atatggctca ggaaggacaa cgtatgcctt
     4981 cctgtatcaa tctgattgga ggcccaagtt ctactgcaga tatagaactg atcaaagttg
     5041 ttggtgtgca tggacctgta catgcaactt acttgattat tgatgactgc taatacattt
     5101 tagtatgtat atatgattaa cagctccgtt tagctgagct gttaatcatt catttagttg
     5161 tattagtcgt gacttgagcg taagcgtcaa tacggactcc gttaatgcct tacaatagca
     5221 gtcagtgttc atgcctgttt attgaggcaa gggggagtcc gggcgcctgt aactaaagat
     5281 gcccccccac gcctttaaac ttctcaataa atgcgctaat cttctgaaaa acggtctgtt
     5341 ttttcgtttt atattgcggg tttaacggac taagttttgg taatgtctcg tttaattctg
     5401 tgccattttc ggtggcgtat tcgcgtttta acgacgtgcg aatatagcgc ctggccgctt
     5461 cttcattaag attttcttct ttgatcaatg catctgcttc acgttgttgt tcgcgttgag
     5521 caaaggtaaa gaacgtatca atgatgctgg ctttgtccgg caaatcatca aggttcgttt
     5581 gctgaataaa atcgaccacc aggccttctt tagcacggtt ccccagactt gaacgaatta
     5641 atcgtttgac ctctacggtc atttcgtcct tacttttgtt ttgtctgctg tgttcgaaaa
     5701 tcaggccaag gatataatcc aggtttattt cctgagactt cagcaaatcg acctcaaaaa
     5761 ccacgtcatc ccagtcagtg gttgattttt cttttttgtc agcttctttt tcgcggtgct
     5821 gccagtcacg aatatcgtta taggcagaac gataatcctg aatcttgcgt tcagcaggga
     5881 gacgaatcat ttgcaattca gcgaactttt catcatccac ataatgttct gctttgaatt
     5941 tttctaccgc aacaggatcg ctgagatcga tttgttgcag ggctttcagc gtggcaaatt
     6001 catcatagtt ttgcaggacg ttctcggcac gcaggtattc accaaacagt ttaacgaagt
     6061 cttttttctc tttttcgctt tcaatactgg caggggcagg gaacctttgt tccagttctg
     6121 aaactactgc cataaagccg cgtttagctt cgccagtggc agcatctgta aagccttcca
     6181 tatactctgc ataactcttt tccagcacca catttctggt gtttttatca ccaaaaagtg
     6241 tgatggcatc gatggttgcc tgctccaggt tacggaatgt gacgatgtta ccgaaggttt
     6301 tcgtggcgtc ataaatgcgg ttggtacggg aaaacgcctg catcagaccg tgaaaacgca
     6361 ggtttttatc gacgaacagc gtgttcagtg ttggagcatc gaagccggtt aaaaacatcc
     6421 ccacgacgat taacaggtca acatcctgat ttttaacccg ctgagccagg tcccgatagt
     6481 agttctgaaa atccttgcta tcagtactga aattcatttg aaaatggtcg ttatattcac
     6541 gaattgcagc atccagaaac tctttagcac tgctgtccat tgcgccggta tcaaaagttt
     6601 catcggaaat ttcaccaatg gcattttgtt cttcattggc ggcaaaggag aatattgtcg
     6661 caatacgcag tggtttataa tttgcagatt tattagcggc ttcctcctgt aagcgtttaa
     6721 acgtcgcgta atagactttc gcagcatcca cgctgcttac tgccagcatg gcattaaaac
     6781 ctctggaacc cgggaaagtg cggtgggtct tctggcggaa gttattcagg atatattgcg
     6841 tgatttcctg aatacgcatg ggatgaagaa acgcctgctg attttcagcc gcactcagtt
     6901 ttttctcgtc agtttctgtc tctaaagact taaactgtgg ccgcacatcg ttgtagtcca
     6961 ccttgaattt gagcactttt tcgtcacgaa tcgcatcggt aatcacatac gaatgcaatt
     7021 cacgaccaaa tacgctggcg gtcgtttctg aacctgaggc gttttccggg aaaacaggtg
     7081 tgccggtaaa accaaactga taatagcgtc tgaatttctt cttcagattt ttctgcgctt
     7141 ctccaaactg gctgcggtgg cattcatcaa atataaacac cacttgctga ttgtatacag
     7201 gcaggtcgct ttctgctttc atcaggttat taagtttctg aatagtggtg acgataattt
     7261 tgttatcgtc cttatccaga tttcgtttaa ggcctgcggt attttccgag ccgttaacac
     7321 tatctggcga aaaacgctga tattccttca tggtctggta atcgaggtct ttcctgtcga
     7381 ccacaaagaa gactttatca ataaagtcca gttctgttgc cagacgcgcg gctttaaagc
     7441 tggtcagtgt tttaccggaa cccgtggtgt gccagatata cccaccgctt tccggttttg
     7501 accagttctt cgctgtaaag gagctcttaa ttttccacag aatgcgctcg gtggcagcaa
     7561 tctggtacgg tcgcatcacc agtagcgtct gactgctgtc aaaaacgctg tagttcacca
     7621 gaacattaag cagggtatgt ttctggaaaa aggtggcggt aaaatctttg aggtctttaa
     7681 tcagcgtgtt gtctgatttc gcccagttca tggtgaagtc aaaactgttt ttatcgcgct
     7741 ttgtcgtgtt ggcaaagtaa cgggtatcgg taccgttgga aatgacgaac agttgcagat
     7801 acttaaacag ggaattttcg ctgttaaaac tctccttgct gtaacgatgt atctggttga
     7861 aagcctcacg aatcgccacg ccacgctttt tcagttcgat ttgcaccagc ggcaaaccat
     7921 taaccaggat agtgacgtca taacggttac tgtgagaacc tgcctgttca aactgccgga
     7981 taatctgcac tttgttgcgc atgagattct ttttatctat cagatagata ttctcaagac
     8041 gcccgttgtc aaaaataaag tcacagatat agtcgatatg gattttacgg gtcttatcca
     8101 gaatgccatc gccagggttg tccagatact gctccgtgaa acgccgccat tcgctgtcat
     8161 taaacaccac gccattgagg ctctgaagct gttcccggac attagccagc atcgccgact
     8221 gtgattttac ggaaataaat tcatagccct gattctgcag gtcctgaatc agttcacgtt
     8281 ccaggtccga ttcactctgg tagctgtcgc ctgtttgctc agccttgatg tacttatcaa
     8341 gaacgataaa gttactggtt tcagcaatgg tgtgtgtctg aagaatcata gcgcatcctt
     8401 tgtgccgtct ggcggggccg gaagggagtt aagggtgact tccggcgtgt aaaaatggtt
     8461 tatatactga ccggatgtta aggtggcccg gtcggtagca attgtcaatt agttactgac
     8521 ggtttcaggt ttcgggaaac tgaacagtaa atcacgatag tactcgtatt gtttctggcg
     8581 caactcgatt tcacgcggga gaccttcggt gatggagttg gtcagggtgt cgaatttatc
     8641 gagtatttcg acaatgcgag cttgttcttc tgaaggtggt acaggtattg ggaattttaa
     8701 aaacattggt cgacgaagtg aggtaaccga tgaacttact gatgcttttt gaatataggt
     8761 aaaaaaatat gctgttatat agtgatatac aaatcgcgga ttcataaatg catttgggac
     8821 aatacgatat gctctctgat gcaaagcata tttaccttta gcgtaatgaa ataccttccc
     8881 caccccggca ccatctccag ccgtaatgat tgctgtttca tcaaaatcaa atacatttaa
     8941 ctttaatggt tcacgtccac gtgcataaaa aatatatttg ccatgttcta tagcatcttg
     9001 cgtatcatgg ttacctgttc ctataacagc cacttccccc aaagtcttcc actcaatccg
     9061 aataccatcc atggcatccg gaaggatgta attacccccc cccgcgcaat atctttgaaa
     9121 ttcaataact tatctagata gtggttgtat tgttttttac gcatgttaag ctctgctgtc
     9181 agctctgctg tcagggcgct gaatttgtcc agaattcgca cgatttcaga ctggatggca
     9241 agggattttt ccggattgtc cgggcagggg attggaactt caaacttatc tgtatcagga
     9301 gttgcaattt gtggcatttg cattttaccc ccaattttct ggaaatatcc ttcatttatt
     9361 tttaggaagt aataaatata ctttatgctt atcgcatcat tatttgagtg atatgaccac
     9421 atctcatttt tgtgagagaa tggtttatcg taatactcga attcgataat acctctggat
     9481 ttaacaataa tcgatggctc tctattaata tctttctctg gtatatcttt aaagtcaaca
     9541 aaggctactg tttttcctcc tgcaaaaatt ttaagcggcg cattatcttt gtgaagagct
     9601 ttcatctgcc cagcggttat ttttgtgcct ttagttcgtt taagcacttt ccctagtgtt
     9661 ttccactcaa cctcagcccc atccagcaat ttttccagat aactcaactc gctcatttct
     9721 gcacctcgca gccttcaatt tcagccacaa tcgcatcaat atctttacgc aactggtcga
     9781 ttttgctgac cgtggttttc agctcagcat tcagctcagc aatatcgata atttcacgag
     9841 tatctttcgc ttccacatag ctgctcaccg acaggttata gtcattagcg acaacggtct
     9901 caaacgcgac tgatttcgcc agatgggcaa catcttcctt gctggcaaat acctgcataa
     9961 tctgttcgat atgagcatcg gtcagaatgt tgttgttagc ctcttttttg aacagttcgc
    10021 tggcatcaat aaactgaact ctggtatccg ttttatgttt agacagcacc agtatgttta
    10081 cggcaatggt ggtgccaaag aacaggttcg gtgccagtga aatcacggtt tcgacatagt
    10141 tattgtcgac cagatactga cggattttct gctctgcgcc gccacggtaa aaaatacccg
    10201 ggaagcagac aatcgcagcg cgacctctgg ccgaaagata gttcagcgca tgtaatacaa
    10261 acgcaaagtc agctttggat ttgggggcca gaacgccagc cggggcaaaa cgctcatcgt
    10321 taatcagtgt cgggtcatcg ctgccagtcc atttcaccga atacggcggg ttagaaacga
    10381 tggcatcaaa cggtttttca tctctgaagt gcggctcagt cagcgtattg cccagcttga
    10441 tatcaaactt gtcgtagttg atgttgtgca aaaacatgtt catacgcgcc aggttatagg
    10501 tcgtatggtt gatttcctga ccaaaaaagc cttcttcgat gatatggtca tcaaactgct
    10561 ttttcgcctg caacaacagc gaaccggaac ccgctgccgg gtcgtagatt ttgttaacgt
    10621 gggtctgccc gtgcatagcc agttgtgcaa tcagcttaga gacgtgctgt ggtgtaaaga
    10681 actcgccgcc tgacttaccg gcatttgcgg catagttaga aataaggaat tcataggcat
    10741 cgccaaacag gtcaatctga tgtttgttga agtcaccaag gtttaaccct tcaacccctt
    10801 tcagaacagc agccaggcgg gcatttttat ccttaacggt gttacccagg cggttactgg
    10861 tggtatcgaa atcagcaaac aaacccttga tgtcagcttc tgaaggataa ccgtaagcag
    10921 aactttcgat agcaacgaag atgctgttta aatctgcatt cagtctgtca ttggtatttg
    10981 ctttcgcagc tacgttgcag aaaagctgac tggggtagat gaagtagcct ttagtcctga
    11041 tggcatc
//
LOCUS       DAZKJR010000160        10986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKJR010000160 DAZKJR010000000
VERSION     DAZKJR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.161"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            315..695
                     /gene="atpI"
                     /locus_tag="ACHOWS_004364"
     CDS             315..695
                     /gene="atpI"
                     /locus_tag="ACHOWS_004364"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFQ0049852.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            704..1519
                     /gene="atpB"
                     /locus_tag="ACHOWS_004365"
     CDS             704..1519
                     /gene="atpB"
                     /locus_tag="ACHOWS_004365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0049853.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1566..1805
                     /gene="atpE"
                     /locus_tag="ACHOWS_004366"
     CDS             1566..1805
                     /gene="atpE"
                     /locus_tag="ACHOWS_004366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFQ0049854.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1867..2337
                     /gene="atpF"
                     /locus_tag="ACHOWS_004367"
     CDS             1867..2337
                     /gene="atpF"
                     /locus_tag="ACHOWS_004367"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0049855.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2352..2885
                     /gene="atpH"
                     /locus_tag="ACHOWS_004368"
     CDS             2352..2885
                     /gene="atpH"
                     /locus_tag="ACHOWS_004368"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0049856.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            2898..4439
                     /gene="atpA"
                     /locus_tag="ACHOWS_004369"
     CDS             2898..4439
                     /gene="atpA"
                     /locus_tag="ACHOWS_004369"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0049857.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4490..5353
                     /gene="atpG"
                     /locus_tag="ACHOWS_004370"
     CDS             4490..5353
                     /gene="atpG"
                     /locus_tag="ACHOWS_004370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0049858.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5380..6762
                     /gene="atpD"
                     /locus_tag="ACHOWS_004371"
     CDS             5380..6762
                     /gene="atpD"
                     /locus_tag="ACHOWS_004371"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0049859.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6783..7202
                     /gene="atpC"
                     /locus_tag="ACHOWS_004372"
     CDS             6783..7202
                     /gene="atpC"
                     /locus_tag="ACHOWS_004372"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0049860.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7551..8921
                     /gene="glmU"
                     /locus_tag="ACHOWS_004373"
     CDS             7551..8921
                     /gene="glmU"
                     /locus_tag="ACHOWS_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0049861.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQSQKEGWR
                     RPVKKK"
     gene            9083..10912
                     /gene="glmS"
                     /locus_tag="ACHOWS_004374"
     CDS             9083..10912
                     /gene="glmS"
                     /locus_tag="ACHOWS_004374"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0049862.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         2635 a         2726 c         3004 g         2621 t
ORIGIN      
        1 caaaggcatc atttgccaag taaataaata tgctgtgcgc gaacatgctc aatatgtgat
       61 ctgaagcacg ctttatcacc agtgtttacg cgttatttac agtttttcat gatcgaacag
      121 ggtttgcaga aaagtcgcaa ttgtatgcac tggaaaaata tttaaacatt tattcacctt
      181 ttggctactt attgtttgaa atcacggggg cgcaccgtat aatttgaccg ctttttgatg
      241 cttgactcta agccttaaag aaagttttat acgacacgcg gcatacctcg aagggagcag
      301 gagtgaaaaa cgtgatgtct gtgtcgctcg tgagtcgaaa cgttgctcgg aagcttctgc
      361 tcgttcagtt actggtggtg atagcaagtg gattgctgtt cagcctcaaa gaccccttct
      421 ggggcgtctc tgcaataagc gggggcctgg cagtttttct gcctaacgtt ttgtttatga
      481 tatttgcctg gcgtcaccag gcgcatacac cagcgaaagg ccgggtggcc tggacattcg
      541 catttggcga agctttcaaa gttctggcga tgttggtgtt actggtggtg gcgttggcgg
      601 ttttaaaggc ggtattcttg ccgctgatcg ttacgtgggt tttggtgctg gtggttcaga
      661 tactggcacc ggctgtaatt aacaacaaag ggtaaaaggc atcatggctt cagaaaatat
      721 gacgccgcag gattacatag gacaccacct gaacaacctt cagctggacc tgcgtacatt
      781 ctcgctggtg gatccacata accccccagc caccttctgg acaatcaata ttgactccat
      841 gttcttctcg gtggtgctgg gtctgttgtt cctggtttta ttccgtagcg tagccaaaaa
      901 ggcgaccagc ggtgtgccag gtaagtttca gaccgcgatt gagctggtga tcggctttgt
      961 taatggtagc gtgaaagaca tgtaccatgg caaaagcaag ctgattgctc cgctggccct
     1021 gacgatcttc gtctgggtat tcctgatgaa cttgatggat ttactgccta tcgacctgct
     1081 gccgtacatt gctgaacatg tactgggtct gcctgcactg cgtgtggttc cgtctgcgga
     1141 cgtgaacgta acgctgtcta tggcactggg tgtatttatc ctgattctgt tctacagcat
     1201 caaaatgaaa ggcatcggcg gcttcacgaa agagttgacg ctgcagccgt tcaatcactg
     1261 ggcgttcatt cctgtcaact taatccttga aggggtaagc ctgctgtcca aaccagtttc
     1321 actcggtttg cgactgttcg gtaacatgta tgccggtgag ctgattttca ttctgattgc
     1381 tggtctgttg ccgtggtggt cacagtggat tctgaatgtg ccgtgggcca ttttccacat
     1441 cctgatcatt acgctgcaag ccttcatctt catggttctg acgatcgtct atctgtcgat
     1501 ggcgtctgaa gaacattaat ttaccaacac tactacgttt taactgaaac aaactggaga
     1561 ctgtcatgga aaacctgaat atggatctgc tgtacatggc tgccgctgtg atgatgggtc
     1621 tggcggcaat cggtgctgcg atcggtatcg gcatcctcgg gggtaaattc ctggaaggcg
     1681 cagcgcgtca acctgatctg attcctctgc tgcgtactca gttctttatc gttatgggtc
     1741 tggtggatgc tatcccgatg atcgctgtag gtctgggtct gtacgtgatg ttcgctgtcg
     1801 cgtagtaagc gttgctttta tttaaagagc aatatcagaa cgttaactaa atagaggcat
     1861 tgtgctgtga atcttaacgc aacaatcctc ggccaggcca tcgcgtttgt cctgttcgtt
     1921 ctgttctgca tgaagtacgt atggccgcca ttaatggcag ccatcgaaaa acgtcaaaaa
     1981 gaaattgctg acggccttgc ttccgcagaa cgagcacata aggaccttga ccttgcaaag
     2041 gccagcgcga ccgaccagct gaaaaaagcg aaagcggaag cccaggtaat catcgagcag
     2101 gctaacaaac gccgctcgca gattctggac gaagcgaaag ctgaggcaga acaggaacgt
     2161 actaaaatcg tggcccaggc gcaggcggaa attgaagccg agcgtaaacg tgcccgtgaa
     2221 gagctgcgta agcaagttgc tatcctggct gttgctggcg ccgagaagat catcgaacgt
     2281 tccgtggatg aagctgctaa cagcgacatc gtggataaac ttgtcgctga actgtaagga
     2341 gggaggggct gatgtctgaa tttattacgg tagctcgccc ctacgccaaa gcagcttttg
     2401 actttgccgt cgaacaccaa agtgtagaac gctggcagga catgctggcg tttgccgccg
     2461 aggtaaccaa aaacgaacaa atggcagagc ttctctctgg cgcgcttgcg ccagaaacgc
     2521 tcgccgagtc gtttatcgca gtttgtggtg agcaactgga cgaaaacggt cagaacctga
     2581 ttcgggttat ggctgaaaat ggtcgtctta acgcgctccc ggatgttctg gagcagttta
     2641 ttcacctgcg tgccgtgagt gaggctaccg ctgaggtaga cgtcatttcc gctgccgcac
     2701 tgagtgaaca acagctcgcg aaaatttctg ctgcgatgga aaaacgtctg tcacgcaaag
     2761 ttaagctgaa ttgcaaaatc gataagtctg taatggcagg cgttatcatc cgagcgggtg
     2821 atatggtcat tgatggcagc gtacgcggtc gtcttgagcg ccttgcagac gtcttgcagt
     2881 cttaagggga ctggagcatg caactgaatt ccaccgaaat cagcgaactg atcaagcagc
     2941 gcattgctca gttcaatgtt gtgagtgaag ctcacaacga aggtactatt gtttctgtaa
     3001 gtgacggtgt tatccgcatt cacggcctgg ccgattgtat gcagggtgaa atgatctccc
     3061 tgccgggtaa ccgttacgct atcgcactga acctcgagcg cgactctgta ggtgcggttg
     3121 ttatgggtcc gtacgctgac cttgccgaag gcatgaaagt taagtgtact ggccgtatcc
     3181 tggaagttcc ggttggccgt ggcctgctgg gccgtgtggt taacactctg ggtgcaccaa
     3241 tcgacggtaa aggtccgctg gatcacgacg gcttctctgc tgtagaagca atcgctccgg
     3301 gcgttatcga acgtcagtcc gtagatcagc cggtacagac cggttataaa gccgttgact
     3361 ccatgatccc aatcggtcgt ggtcagcgtg aattgatcat cggtgaccgt cagaccggta
     3421 aaaccgcact ggctatcgat gccatcatca accagcgcga ttccggtatc aaatgtatct
     3481 atgtcgctat cggccagaaa gcgtccacca tttctaacgt ggtacgtaaa ctggaagagc
     3541 acggcgcact ggctaacacc atcgttgtgg tagcaaccgc gtctgaatcc gctgcactgc
     3601 aatacctggc accgtacgct ggttgtgcaa tgggcgaata cttccgtgac cgcggtgaag
     3661 atgcgctgat catttacgat gacctgtcta aacaggctgt tgcttaccgt cagatctccc
     3721 tgctgctccg tcgtccgcca ggacgtgaag cattcccggg cgacgtattc tacctccact
     3781 ctcgtctgct ggagcgtgct gcgcgtgtta acgccgaata cgttgaagcc ttcaccaaag
     3841 gtgaagtgaa agggaaaacc ggttctctga ctgcgctgcc gattatcgaa actcaggcgg
     3901 gtgacgtttc tgcgttcgtt ccaaccaacg taatctccat taccgatggt cagatcttcc
     3961 tggaaaccaa cctgttcaac gccggtattc gtcctgcggt taacccgggt atttccgtat
     4021 cccgtgttgg tggtgcagca cagaccaaga tcatgaaaaa actgtccggt ggtatccgta
     4081 ccgctctggc acagtatcgt gaactggcag cgttctctca gtttgcatcc gaccttgacg
     4141 atgcaacacg taagcagctt gaccacggtc agaaagtgac cgaactgctg aagcagaaac
     4201 agtatgcgcc gatgtctgtt gcgcaacagt ctctggttct gttcgcagca gaacgtggtt
     4261 acctggcgga tgtagaactg tcgaaaatcg gcagcttcga agccgctctg ctggcttacg
     4321 tcgaccgtga tcacgctccg ttgatgcaag agatcaacca gaccggtggc tacaacgacg
     4381 aaatcgaagg caagctgaaa ggcatcctcg attccttcaa agcaacccaa tcctggtaac
     4441 gtctggcggc ttgccttagg gcaggccgca aggcattgag gagaagctca tggccggcgc
     4501 aaaagagata cgtagtaaga tcgcaagcgt ccagaacacg caaaagatca ctaaagcgat
     4561 ggagatggtc gccgcttcca aaatgcgtaa atcgcaggat cgcatggcgg ccagccgtcc
     4621 ttatgcagaa accatgcgca aagtgattgg tcaccttgca cacggtaatc tggaatataa
     4681 gcacccttac ctggaagacc gcgacgttaa acgcgtgggc tacctggtgg tgtcgaccga
     4741 ccgtggtttg tgcggtggtt tgaacattaa cctgttcaaa aaactgctgg cggaaatgaa
     4801 gacctggacc gacaaaggcg ttcaatgcga cctcgcaatg atcggctcga aaggcgtgtc
     4861 gttcttcaac tccgtgggcg gcaatgttgt tgcccaggtc accggcatgg gggataaccc
     4921 ttccctgtcc gaactgatcg gtccggtaaa agtgatgttg caggcctacg acgaaggccg
     4981 tctggacaag ctttacattg tcagcaacaa atttattaac accatgtctc aggttccgac
     5041 catcagccag ctgctgccgt taccggcatc agatgatgat gatctgaaac ataaatcctg
     5101 ggattacctg tacgaacccg atccgaaggc gttgctggat accctgctgc gtcgttatgt
     5161 cgaatctcag gtttatcagg gcgtggttga aaacctggcc agcgagcagg ccgcccgtat
     5221 ggtggcgatg aaagccgcga ccgacaatgg cggcagcctg attaaagagc tgcagttggt
     5281 atacaacaaa gctcgtcagg ccagcattac tcaggaactc accgagatcg tctcgggggc
     5341 cgccgcggtt taaacaggtt atttcgtaga ggatttaaga tggctactgg aaagattgtc
     5401 caggtaatcg gcgccgtagt tgacgtcgaa ttccctcagg atgccgtacc gcgcgtgtac
     5461 gatgctcttg aggtgcaaaa tggtaatgag cgtctggtgc tggaagttca gcagcagctc
     5521 ggcggcggta tcgtacgtac catcgcaatg ggttcctccg acggtctgcg tcgcggtctg
     5581 gatgtaaaag acctcgaaca cccgatcgaa gtcccggtag gtaaagcgac tctgggccgt
     5641 atcatgaacg tactgggtga accggtcgac atgaaaggcg agatcggtga agaagagcgt
     5701 tgggcgattc accgcgcagc accttcctac gaagagctgt caaactctca ggaactgctg
     5761 gaaaccggta tcaaagttat cgacctgatg tgtccgttcg ctaagggcgg taaagttggt
     5821 ctgttcggtg gtgcgggtgt aggtaaaacc gtaaacatga tggagcttat tcgtaacatc
     5881 gcgatcgagc actccggtta ctctgtgttt gcgggcgtag gtgaacgtac tcgtgagggt
     5941 aacgacttct accacgaaat gaccgactcc aacgttatcg acaaagtatc cctggtgtat
     6001 ggccagatga acgagccgcc gggaaaccgt ctgcgcgttg ctctgaccgg tctgaccatg
     6061 gctgagaaat tccgtgacga aggtcgtgac gttctgctgt tcgttgacaa catctatcgt
     6121 tacaccctgg ccggtacgga agtatccgca ctgctgggcc gtatgccttc agcggtaggt
     6181 tatcagccga ccctggcgga agagatgggc gttctgcagg aacgtatcac ctccaccaaa
     6241 accggttcta tcacctccgt acaggcggta tacgtacctg cggatgactt gactgacccg
     6301 tctccggcaa ccacctttgc gcaccttgac gcaaccgtgg tactgagccg tcagatcgcg
     6361 tctctgggta tctacccggc cgttgacccg ctggactcca ccagccgtca gctggacccg
     6421 ctggtggttg gtcaggaaca ctacgacacc gcgcgtggcg ttcagtccat cctgcaacgt
     6481 tatcaggaac tgaaagacat tatcgccatc ctgggtatgg atgaactgtc tgaagaagac
     6541 aaactggtgg tagcgcgtgc tcgtaagatc cagcgcttcc tgtcccagcc gttcttcgtg
     6601 gcagaagtat tcaccggttc tccgggtaaa tacgtctccc tgaaagacac catccgtggc
     6661 tttaaaggca tcatggaagg cgaatacgat cacctgccgg agcaggcgtt ctacatggtc
     6721 ggttccatcg aagaagctgt ggaaaaagcc aaaaaacttt aacgccttaa tcggagggtg
     6781 atatggcaat gacttaccac ctggacgtcg tcagtgcaga gcaacaaatg ttctctggtc
     6841 tggtcgagaa aatccaggta acgggtagcg aaggtgaact ggggatctat cctggccacg
     6901 caccgctgct caccgccatt aagcctggta tgattcgcat cgtgaaacag cacggtcacg
     6961 aagagtttat ctatctgtct ggcggcattc ttgaagtgca gcctggcaac gtgaccgttc
     7021 tggccgacac cgcaattcgc ggccaggatc tcgacgaagc gcgagccatg gaagcgaaac
     7081 gtaaggctga agagcacatt agcagctctc acggcgacgt agattacgct caggcgtctg
     7141 cggaactggc caaagcgatc gcgcagctgc gcgttatcga gttgaccaaa aaagcgatgt
     7201 aacaccggct tgaaaagcac aaaagccagt ctggaaacag gctggctttt tttacgcgtg
     7261 acccgtcctg aagaacgttc acatagatcc tgctgatata aaaccccctg ttttcccgtt
     7321 tattcattga tcgaaataag agcaaaaaca tccacctgac gcttaaatga aggtactgcc
     7381 ttaattttct gcagacaaaa ggtgtgacga tggtcgaaaa tggcgctttc gtcagcgggg
     7441 ataatccgtt attgaacaat ttatcctctg tccatttcac gatgaaaaaa atgtagtttt
     7501 ttcaaggtga agcggtttaa attcgttctc aaattacagt caggacgcgt atgttgaata
     7561 atgctatgag cgtagtgatc cttgccgcag gcaaaggcac gcgcatgtat tccgatcttc
     7621 cgaaagtgct gcataccctt gccgggaaag cgatggttca gcatgtcatt gatgctgcga
     7681 atgaattagg cgcagcgcac gttcacctgg tttacggtca cggcggcgat ctgcttaaac
     7741 aggcgctgaa agatgacaac ctgaactggg tgcttcaggc agaacagctg ggtacaggtc
     7801 atgcgatgca gcaggccgca cctttctttg ccgatgatga agacatttta atgctctacg
     7861 gcgacgtgcc gctgatctct gtcgaaacac tccagcgtct gcgtgatgct aaaccgcagg
     7921 gtggcattgg tctgctgacg gtaaaactgg atgatccgac cggttatgga cgtatcaccc
     7981 gtgaaaacgg caaagttacc ggcattgttg agcacaaaga cgccaccgac gagcagcgtc
     8041 agattcagga gatcaacacc ggcattctga ttgccaacgg cgcagatatg aaacgctggc
     8101 tggcgaagct gaccaacaat aacgctcagg gtgaatacta catcaccgac attattgcgc
     8161 tggcgtatca ggaagggcgt gaaatcgtcg ccgttcatcc gcaacgttta agcgaagtag
     8221 aaggcgtgaa taaccgcctg caactctccc gactggagcg cgtttaccag tctgaacagg
     8281 ctgaaaaact gctgttagca ggcgttatgc tgcgcgatcc ggcgcgtttt gatctgcgcg
     8341 gtacgcttac tcacgggcgc gatgttgaaa ttgatactaa cgttatcatc gagggcaacg
     8401 tgactctcgg tcatcgcgtg aaaatcggca ccggttgcgt gattaaaaac agcgtgattg
     8461 gcgatgattg cgaaatcagt ccgtataccg ttgtggaaga tgcgaatctg gcagcggcct
     8521 gtaccattgg cccgtttgcc cgtctgcgtc ctggtgctga gttgctggaa ggtgcacacg
     8581 tcggtaactt cgttgagatg aaaaaagcgc gtctgggtaa aggctcgaaa gccgggcatc
     8641 tgacttatct gggcgatgcg gaaattggcg ataacgttaa catcggcgcg ggaaccatta
     8701 cctgcaacta cgacggtgcg aataaattta agaccattat cggcgacgat gtgtttgttg
     8761 gttccgatac tcagctggtg gccccggtaa cggtaggcaa aggcgcaacc attgctgcgg
     8821 gtacaactgt gacgcgtaat gtcggcgaaa atgctctggc aatcagccgt gtgccgcagt
     8881 cgcaaaaaga aggctggcgt cgtccggtaa agaaaaagta attctggctg ggaaatccgg
     8941 tcatatggga tgaggagata acataatctc ccgcccacaa gcagtaacta taaaaataac
     9001 cccactctct acaaggctcg gggcgcccga aaaaacgggc atacaggttg accgacaacg
     9061 atataaatcg gaatcaaaaa ctatgtgtgg aattgttggc gcgatcgcgc aacgtgatgt
     9121 agcagaaatc cttcttgaag gtttacgtcg tctggaatac cgcggatatg actctgccgg
     9181 tctggccgtt gttgatgcag aaggtcatat gacccgcctg cgtcgcctcg gtaaagtaca
     9241 gatgctggct caggcagcgg aagaacatcc tctgcatggc ggcaccggta ttgctcatac
     9301 tcgctgggcg acccacggtg aaccttcaga agctaacgcg catccgcatg tttctgaaca
     9361 cattgtggtg gtgcataacg gcatcatcga aaaccatgaa ccgctgcgtg aagagttaaa
     9421 agcgcgtggc tataccttcg tttctgaaac cgacaccgaa gtgattgcac atttggtgaa
     9481 ctgggagctg aaacaaggcg gtactctgcg tgaggcagtt ctgcgtgcta ttccgcaact
     9541 gcgtggtgcg tacggtacag tgatcatgga ctcccgtcac ccggataccc tgctggcggc
     9601 acgttctggt agcccgctgg tgattggtct gggcatgggc gaaaacttta tcgcttctga
     9661 ccagctggcg ctgttgccag tgactcgtcg ctttatcttc cttgaagagg gcgatattgc
     9721 ggaaatcact cgccgttcgg taaacatttt cgataaaact ggcgcggaag taaaacgtca
     9781 ggatattgaa tccaatctgc aatatgacgc gggcgataaa ggtatttacc gtcactacat
     9841 gcagaaagag atctacgaac aaccgaacgc gatcaaaaac acccttaccg ggcgcatcag
     9901 ccacggtcag gttgatttaa gcgagctggg accgaacgcc gacgaactgc tgtcgaaggt
     9961 tgagcatatt cagatcctcg cctgtggtac ttcttataac tccggtatgg tttcccgcta
    10021 ctggtttgaa tcgctggcag gtattccgtg cgacgtcgaa atcgcctctg aattccgcta
    10081 tcgcaaatct gccgtgcgtc gtaacagcct gatgatcacc ttgtcacagt ctggcgaaac
    10141 cgcggatacc ctggcgggcc tgcgtctgtc gaaagagctg ggttaccttg gttcgctggc
    10201 aatctgtaac gttccgggtt cttctctggt gcgcgaatcc gatctggcgc tgatgaccaa
    10261 cgccggtacg gaaatcggcg tggcatccac taaagcattc accactcagt taactgtgct
    10321 gttgatgctg gtggcgaagc tgtctcgcct gaaaggtctg gatgcctcca ttgaacatga
    10381 cattgtgcat ggtctgcagg cgctgccgag ccgtattgag cagatgctgt ctcaggacaa
    10441 acgcattgaa gcgctggcag aagatttctc tgacaaacat cacgcgctgt tcctgggccg
    10501 tggcgatcag tacccaatcg cgctggaagg tgcattgaag ctgaaagaga tctcttacat
    10561 tcacgctgaa gcctacgctg cgggcgaact gaaacacggc ccgctggcgc tgattgacgc
    10621 cgatatgccg gttatcgtcg ttgcgccgaa caacgagttg ttggaaaaac tgaaatctaa
    10681 cattgaagaa gttcgcgcgc gtggcggtca gttgtatgtc ttcgccgatc aggatgcggg
    10741 ttttgtgagc agtgacaaca tgcacatcat tgagatgccg catgtggaag aggtgattgc
    10801 accaatcttc tacaccgttc cgctgcagct actggcttac cacgttgcgc tgatcaaagg
    10861 caccgacgtt gaccagccgc gtaacctggc gaaatcggtt acggttgagt aatatctctc
    10921 gttgatagaa aagggctgcc gaccggcggc ccttttttat aagtgatatc aataattcca
    10981 ttattc
//
LOCUS       DAZKJR010000161        10910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKJR010000161 DAZKJR010000000
VERSION     DAZKJR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.162"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            435..1256
                     /locus_tag="ACHOWS_004375"
     CDS             435..1256
                     /locus_tag="ACHOWS_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFQ0049863.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIIIWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            1346..2548
                     /locus_tag="ACHOWS_004376"
     CDS             1346..2548
                     /locus_tag="ACHOWS_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFQ0049864.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLLPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            2688..2858
                     /locus_tag="ACHOWS_004377"
     CDS             2688..2858
                     /locus_tag="ACHOWS_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049865.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVFQPFSKNTNQ"
     gene            2907..3359
                     /locus_tag="ACHOWS_004378"
     CDS             2907..3359
                     /locus_tag="ACHOWS_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFQ0049866.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            3356..4192
                     /locus_tag="ACHOWS_004379"
     CDS             3356..4192
                     /locus_tag="ACHOWS_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFQ0049867.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            4176..5324
                     /gene="traJ"
                     /locus_tag="ACHOWS_004380"
     CDS             4176..5324
                     /gene="traJ"
                     /locus_tag="ACHOWS_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFQ0049868.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            5321..5611
                     /locus_tag="ACHOWS_004381"
     CDS             5321..5611
                     /locus_tag="ACHOWS_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0049869.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            5675..9692
                     /locus_tag="ACHOWS_004382"
                     /pseudo
     CDS             5675..9692
                     /locus_tag="ACHOWS_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
     gene            9709..10059
                     /locus_tag="ACHOWS_004383"
     CDS             9709..10059
                     /locus_tag="ACHOWS_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0049870.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            10071..10766
                     /locus_tag="ACHOWS_004384"
     CDS             10071..10766
                     /locus_tag="ACHOWS_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFQ0049871.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            10777..>10910
                     /locus_tag="ACHOWS_004385"
     CDS             10777..>10910
                     /locus_tag="ACHOWS_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFQ0049872.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQAPGNA
                     P"
BASE COUNT         2837 a         3151 c         2686 g         2236 t
ORIGIN      
        1 ctgcaaaaga aacacacacg gcccccataa aaataaatgt catcgaatcc catggtttca
       61 ccagtacaac cggaaccatg gctgccagaa caataagagc agcattaccc aacagcgtct
      121 ctctaataat atcccttata atctccagtt tttctttatg ctgtttaatt ttcatacgaa
      181 cctcgttgat ttcatcccga aaaggaaaag attatataac gaaatacttt caatctcctg
      241 actccgtctg ctctgcagca taatgccaga ccagcggcat gctaaatcac gtcctgtccg
      301 aatccttttc attcacgctt aaaacagagt taaaagcact cttgaaacac actcgatcat
      361 aacgatcgaa ataactcgtt tccgttcagt aaacacgctc tttagactta acatatgaac
      421 cgcacggagg tgaaatgaaa cccaacagca caggcattgc tgcccgcatg atgctctcca
      481 ttgacagggc ccggattagc gaagctctgc tcaataaccg ccagttgcag tccactcccc
      541 tgcagatccg ttatccgcag ggcgtcagag aagcactcgg aataatgagc gagcaactct
      601 ccctttctgt atccgacctg acgcgtatcc tggtcgaaga tgcattaact gaaatgtttc
      661 tcccggctga aaacatcgtt caccgtctta tctgccggac agaacacatc atgcaggcac
      721 atgatgtctg cgtaacaacg atgacaacgt tactggctcc ctggaatatc cgtcctgccg
      781 ttttccgtga cccggacaaa ctcgcagatt atctgacagg agagattctg actgctctgg
      841 ctgactggtt ttatctcaac ccggaatggc tgaacggcag ggtacgttat cctttaagcc
      901 gtgccggtga gtggccagca acacaggatg cgttctgcca gcttatttca gccagcgata
      961 atgtggatat tattatctgg cacagttttc cttttgccgg tgttcattca caggaatatt
     1021 gcggtgtact gttccgcaag aaaaaagaaa ttaataaagc catcatttat cctgtacttt
     1081 ctttatcacc tgtaagaatg aataaggaaa aagaaaactg gtttcacact gcccgcaatt
     1141 tatatgcaaa gacatccgtc cggaccgtaa cgcttcgtcc ggcacaggca gaatatctga
     1201 tgacaggaaa aatattacct gctgagttat tcagtatacc gttattaccc tggtaataac
     1261 gcagagaaaa aaccaataaa tttttaatgg cacagtcgtg tcataccgtt gctgtaccca
     1321 aaacacctca aaccaggaaa ctgaaatgat gatgaatatg cacattaccc gaactctcat
     1381 cgcatctgct gttttatttt cctttaattc aacagccgca acttcatatt ttgaagcccg
     1441 caatgatgca atggggggaa ccggggtagc ctcctctcac tacggtgtgg cacctctggc
     1501 aaacccggcc ctgctgacaa aagccggtcc ggaagatgac ttcagtcttc tgcttccgtc
     1561 cgttggtggg caattgtccg atccgggtaa tatcagtgac aacgcagaca aaatcagtga
     1621 tgactggaaa gcctttgatc gtgccatcga cagtcagagc aacgtgtcgc agactgctgc
     1681 acgtctgaag acgcgacttc aggacttccg tcacacgcat gctgacgcac aggtgggaac
     1741 atctgctgtg gcgactcttc ccggtgacac cctcgctgcc gctctgatgc tgaaaagcta
     1801 cggtacggtc agtgtcgacg gtaaagtcag tgatgccgac ctgaactatc tggaaagcat
     1861 tgcaaacagt aacggtcagg atgtggataa agacagactg acctctcagg cttttgcccg
     1921 tgccgcactg gtcaccgatg tgggtattgc tctggctact gaactggaaa cggccggaca
     1981 aaagtggtct ctgggcttca caccaaaatt ccagcgtgtt gacctgttta actacaacgt
     2041 gctggttaaa aattacgaca gcagcgcttt caaaggcgac cgttaccaca acaccagaaa
     2101 cggcattaac gctgatatcg gggccagcat ggacctggat gacaactgga cactgggtct
     2161 ggtcgcacag aacctgctcc cccgcagtat tgagacaaag gaagtgaacg gtgtcactga
     2221 aactttcagg atccgcccgc aggtgactgc cggtgtctcc tggcacaacg acatgttcac
     2281 tacagcactg gatgttgacc tgaccccggc cagtggcttc acatcagaca gtaaacgtca
     2341 gtttgctgcc attggtgctg aatttaatgc ctggaaatgg gcacagttac gtgcaggcta
     2401 ccgccagaat ctggccagca atgatggcag tgcgttcacc gccgggctgg gtatctcacc
     2461 atttgatgtc gtccatattg atgtggcggg tatagcaggc acagacaaca actacggtgc
     2521 cgttgcacaa ctgcagttca cattctgatc atctctgccc cggtactgac cggggtatcc
     2581 agacctgcat cctcgcgttt tccgaccatc aaacgacaca gccagacctg tagtgctgcc
     2641 tctgatctgg ctgttatctg acatatcatt ttcgcatgag gaaatgcatg tttaacaaaa
     2701 ataaaacgca tatcgaaaac aaagccttta tggtggaacc gttccgggat ccgacagcta
     2761 acgaatgtaa tgtcgtgata gcaaacgata tttgtatccg ggtagcactg tcaccctctg
     2821 aggtgttcca gccattcagc aaaaacacga accagtaaat cctccccaca acgctttctt
     2881 ctttcactgc tcaggaaaaa tacaccatga accgtatgat acctgcggga tgtctcgccg
     2941 tactctgcct gaacggctgc catctccccc cggataatac agctccgacc agaaagtatg
     3001 gtgctgcctc tgatgtcgcc gtcgcccggc atgcgcagta tcagctatgg gggcagggaa
     3061 tttactctgc agcccttccg gagcgtcagt ccggcggcag tctgaaggct cacagcgacc
     3121 gtgtttcctt tgactgggaa ggcgatgccc tggaattgct caacgaactg gcccgtctgc
     3181 gcggccagca gttcagctac agcggcgtcc gtctcccctt acccgtctca ctgcacgtcc
     3241 gcgacatgac ctttgctgat gtccttcgtc tcactgaagc ccagactgcc tggcgggcaa
     3301 cactctcgca gtcagccggg cgtttacatc tgtcttttat gacgccggag aaaaaatgaa
     3361 aacacacctg tgtctcctgc tgtctgctct cctgcttaca ggctgtgccg gacagcatca
     3421 ccgttcttct gccgtcatga cagaaaacgc accgcccccg gatatccgtg catggctgac
     3481 gccctccgga aaccaggatg atggtatttc agatacccgt cgtcagatgc tcacggatgc
     3541 cggcagaacg ctgggattcc gtggaggaaa agcacagcgc tcatgggaac tgattcaggc
     3601 actgaatgcc cgggaaagta ccctgaatgc cctgtatgat ttccggcctc tcatcagcaa
     3661 ggaaggctgg ctgcctcctg ttatcgatga agcgcaggat gttgcacaca tcagaccgga
     3721 ccagataaga acagcctccc gtgtctggac catcctccgc ccggaacgct ttgtcagtaa
     3781 tccacctgga tggcgtgact ggctgctgaa aggactctca accaccgcca caccaggcac
     3841 tgaaggccgg gttgtgccgg aagaccgggc acaacgcaga ctatgggaaa acgccttacg
     3901 tcagggatgg caggaagggc gcctcaacgc agatctcaca ctggaagcta atcagaagcg
     3961 cctgacccgt gattaccggg gcatgatgct ttacgccctt ctgtggcgtc agggcatgat
     4021 cacacgtcca gatgtcacag agcagatgca gacggttacc ggcaacgggc aaaaactcat
     4081 cactggcgat cgcgtccgcc gcctgaaaac acatgctgaa tttacgctgc agaaatcgcg
     4141 ctggcgccct cttgtcagca ccgaaggaga gaaaaatgat atcaccagat gacttcggta
     4201 ccttcccctt ctcccgtttt acggcagaag agtttcgcca tttttttgcc tggtgtgccc
     4261 gccaccgcgt cagtgatatt gatctgaccg gcggttcacc ggtatccgtc agtcgctttg
     4321 gacggcgggt tcgctcatcc gtaaatcccc ttccgtcctc catcatgatg gcactgagtg
     4381 atgagctgtt tgggcgggag gttcttcccc gggttctgtc aggtcatccc gtggatcgta
     4441 ccattcaggt cagcggagat gcctccggtc gctatggcct gcagcgaggt cagcgtgttc
     4501 gcctgcgctg ccacctgata cagggaacat cggcaacaga agaaaaagcc atttccgtca
     4561 ccatgcgggt gatcccgtca gatattccgg acatcctcac tatgaatatt gaaccggatc
     4621 tgctggatgc catgctcaga aaaaccggcc tgggcttcat ctgcggagaa accggctccg
     4681 ggaaatccac actggcggcg gcactgtatc gccacattct gaaaaatttc cccgatcgca
     4741 aaatagtgac ctatgaagat ccggtggaat acattctggg cggagagaat gaccttctgc
     4801 ctccccatca ggccgaaatc gggcgcgatg tggaaagttt tgctgcagga cttcgctctg
     4861 ccgttcgccg taatccggac atcattggta tcggtgaaat cagggattat gaaacagcag
     4921 atgccgcagt ccaggcgggt aataccggac acttttgtat cggcaccatg cacacaaaat
     4981 cacccggaga aacctttgcc cgcctgctgg gattgtttcc tcctgaaatc agggattcga
     5041 tggctgcagc cacgctttcc ctcgtacagt tcattctggt gcaggtactg gtcagaacaa
     5101 atgacggcgg caggcaggct gtccgggaat acatcgtcat gactgacgaa ctgcgcaata
     5161 cgctctccgg acaatcccat gctacatggg gacattacat tgacgaaatc attcgtcagg
     5221 aaaaacggcg gataagggat caggttctga ccatgtatca gaacgaccgc attgagaaat
     5281 cagaagcagc gctcttcatc ccggccggag agttcccccg atgaaacgcg aaggcttctg
     5341 gcgggatgta acaccaccgc tcaacattta tggtgtcccc ctgccgttta ccatgattta
     5401 cctgctgttg tttccgtatc cgtcaaaaac gacattctgg gtgtgcagcg gcattctgct
     5461 gttttttatg ctcctgggat attccggctg gacagtccgc accctctgta tacgtctgta
     5521 cagcctgctt cagggtaagc aggcatcggg acgtccctgg tggtaccgac accgcacaga
     5581 attaccccgt gaatggcacg gaccagaata gccctctttt gtccatcatc ccggcgtatc
     5641 acaacgccct catatttaat ccccggagaa cgacatgaag tccagatatc gtacctggct
     5701 tgctgtcccg cctgaggaga cagaagccgt aaaaaatgca gtccccccgt taaatggccg
     5761 caaagcggta gcatgggatc cagagaaaaa actctggtat gccgaggcgg gtaccgacct
     5821 ttcactgctg aagcgctggt taccccgtcc acaggaaatg tcgatggatg ccggcgatcc
     5881 ggtcacagaa tttgctcagg tcctggaaaa cgccgggctg gttattcagg gattgccaca
     5941 gatggatggc gccatacaca gagtcgccac ccgggatgac aaaaaagggg cccaaagtgg
     6001 cgcatacaag gcctatcttg atggccgccc ctccggctgg taccgcgatt accggagcgc
     6061 tgataacagt cccacaacct ggacattctc ccgcgcagaa aatatcgatc ctctcgccag
     6121 actgcatctg aaggcatccg ctcagcaaaa tcgcgatgag aaagaacgta aactacagca
     6181 acagtacaac aaacaggccg gctatgcccg ctcctatctt aacgggttgc cccaggcaac
     6241 agcacatgaa tacctgacac gcaagggcat ccgggccgcg cctggcgtgc ggctgaacaa
     6301 taaaaatgag ctggttattc ccttcagcaa cggccggggg gaaatacgtt cgtaccagcg
     6361 tattccggtc accggtggga aggatgcccg cattctgaaa ggctctgaga aaagcggcaa
     6421 ctggttcgcc ttcggaaccc cggaaaatgg ccgtcccctg ttatttgcgg aaggttacgc
     6481 tactgccgcc tccctgcacg aagcaaccgg ccttcctgtt ctgatgacaa ttgatgccga
     6541 caatatgatt aatgtggcaa aaaatgcccg caaaatctgg actgacagtc cctttgtctt
     6601 ctgtgccgac aacgaccatc aacggaaaat taataaaggt gtttcaagtg caaaaaaagc
     6661 tgccgcaagc acgcatggcg aagtcattat tccgaccttt acggaggcgg aaaaagcaca
     6721 gggactgacc gatttcaatg acctggacgc cagccgggga cgagacgcct ttcagcgagc
     6781 catgaacgca cagttagaat atatcggtat ccccacgccc aacaatgaca ctactgaccg
     6841 ccgtgaagct atcgtgatcg gcaacctgat attcacaccg gtaaaagatg agaagccaca
     6901 agtgtcccct gaaaacagac agagcacagc ccctgaaccg gcagtgtcgg aacagaatca
     6961 tccttatgac atccctgcag aagagacaga caatcaggca tatgaagatg actatgcagc
     7021 atatgcatcc ctgcagtcgg taactgcagg gacagcctcc ccgaatgaac cgcaggtgaa
     7081 tgctgtttct gccattgaca gcaacgcaaa gaatacctct gccgccacca ctgacgaccg
     7141 aaatgtatta gcacaagaaa ccaatgctga cgccacggtt acgaccacgg ctcacgacac
     7201 cggcacagca gaaacagatt caccttctga acctgcagag attcagccag tgcagcaggc
     7261 cagtcctgaa ccatcggcta aagataaacc acaggagaac gtagtgtctg ctactgataa
     7321 tgacgcagag aacacctctt ccaccaccac tgccctccgg agttacgaca caccggaaac
     7381 atccgtctcc gacgcaaccg ggacacagca caccgccaca acgcaacctg aagaaccaca
     7441 gcaggtctcc atgggcagcg ctgacgactg gattgcattt gcggaagacc tcaatgctga
     7501 tgctccgggg aatgccactg ttcacagcac cgacaacgcg gagacaaaga cactttctga
     7561 acctgccggg atgcaccctg ttcaacaggc aacccctgaa ccaacggaag aaaataaacc
     7621 tcaggaagag ggcgttatgt ctgccactga tacaggtgaa gagagtgccg ctgccgctat
     7681 cgatgacgac ctgaacacag ttgcggaaga cctcaatgct gatgttactg tgatgaccac
     7741 agctctccac accgatacag cagaaacagc ctcaccttct gaaccagcag aaattcagcc
     7801 cgtacagcag gcaacccctg agccaacagc agaagataaa ccgaaggaag agcgtcctgt
     7861 gtctgccact gagaccagca cagagaatga agcagcccgc cctgaaacac catcgctttt
     7921 cagccgcctg cgccgttttc tgaccggtgg aaaatcacgt accgccgaac ctcccgttat
     7981 tcaggagaac acgcttacgg aggccgaaac tgcgacagag gccgcagcac caccgtcacc
     8041 acctgaaacc agcccggaag acacatcgtc agcagaaccg gacacgatta tctttgcgcc
     8101 ccgccggccc aacagtccgc agcggcacaa cctggacgag attattaaaa atcttacata
     8161 cgatgagtta cccgacaata ctgcgctctg ccgcgtcgac ggcgaaccgg cattcctgga
     8221 taaactcaac cgtctggaaa tggtggatgg tgccagtgct gatgacaaaa aggtcctggc
     8281 tgcccttgct gtggccacca acttctatgg cggcgttatc gagctgaccg gcagtgatgc
     8341 ctttaaacaa aaagccatgc agctgattgt cgaatatgac atcaaggttc gcatgaaact
     8401 cccggtccag agggctgcgc tggaaaaact ccgccaggag aaagggatag cgcatgatgc
     8461 catcgtcacc cacagaccca cgccggaact caaccgcctg tcaccggaag agacaacaat
     8521 ggcacaaccg gcccccccct gtgtcaccga aagcagaaac agagcaaact gcaggcagta
     8581 cggctgaaac accgcatccg gaagcgccgg tcacggcaaa agctgaagcc ccccgagccg
     8641 gcacaccgga acaacccgcc gcctcgtcgt ctgcgccaga aaaagcccct gtcagtgcac
     8701 agccgcagca acccgacaca ccggaggaag agccgcccgg caagctcaga cccggtgaaa
     8761 gtgtgactgc cgtcctgaca aactacggag aaaaagagta tgcgcccgga aaagggcgct
     8821 gcttttttgt ggagctcacc aaccgcagtg gcaaacggga atactgggga aacggcctgg
     8881 aagaactggt caaaaaccac cagaaaggca accccgtcac actgacgctg caggcgagag
     8941 aaacattccg gctgtccggt gaaacacggg atcgcatccg taatatctgg gccatgactc
     9001 ctgtcagtaa cggcattacc gtcgataatg accatcctca tgctggtcag catatcctgg
     9061 aatttccgat gaaaacattc agtcaggtaa tgtcacaaat gcaacaacac tggccacaga
     9121 taatgcagga agttcgctcc cccaaaaatc ctcccaataa tctgtacatt ggtgaagaca
     9181 ggcaacctgc atcagcacct caggatcaat caatgcgact ccccctcgct ggtaatgccc
     9241 ccaatgaact gataccagcc atcgcctctg ccgataagga caatcgtcag ttgaatcttc
     9301 tgctggtcca cagcgcagat gatcatctgc agggcgtggt ccgcctgaac ggcacccttt
     9361 acccggcact ggccaccccg tcagctgaca accgtcagct ggtcatcaat gccctgacgg
     9421 ataacggtct gcagtttgcc ggttacggtg aagctgttaa ccatgatgag aatactcatc
     9481 aacgtccgtc cccacaaata atgcagtttc acctgaagca acaggactcc ccgttgtttg
     9541 ccgccatcca caaaccggaa gagcaaccgg acaagctttt ccgctctctg ggttttgagc
     9601 agacctggaa agagtggtct gacagccaaa aggcggagga caggcaggaa aaaactctgc
     9661 aacaggcaca atcccacagc cccggcctgt gagccggtaa ggacgaccat gaacatgaaa
     9721 aaaacgctgc tggtcatact gaccagcatc tcgctggctt ctccggctgc cgtggcagca
     9781 acctgtcggg caggtgctgc cgcagaagcc ggcagcaaag ccgcctggga gcgggcgcgc
     9841 acagccagcc aggcatggtc acagcgagaa aacgacgtcg cctcctccct gcagtcctgc
     9901 ctgtcccgca ttaaggacgt cagcatcact cttccacaat tccccagcct ggacaaaatc
     9961 cttcgcgatc tggaaaacca ggtctgtgat gccgtcgtgg atcgggtgaa tgaacacctg
    10021 ccggggaata tcgacccctg gaaagactat aacctctgaa caggagcccc atgagcgaag
    10081 cacaccccac atcccctgtc tctccggaaa cagataacag cggagatatt tataaacaaa
    10141 cgctcagtac cattgcacag tcagaacaac gggccgcact ggttcccgga ctggtcaggg
    10201 ccttgctgtg tagcagcgca gctctcgtca tcagtctgac cggcaatatc atacagtact
    10261 ggcataacag caacgtaacg cgtgaatact ttgccaccga caatggccgg ctggtccgcc
    10321 tggcccccac cagtcagcca gcctggagtc agagcgacgt catgacgttt ggcagcatga
    10381 cgctgagcga agcttttaat ctggattttg tccactacag gaaacaaatc acgacactgg
    10441 ccccccgctt ttccgaagcc ggttttgccg gctatgtgaa tgctcttcag gcctccaaca
    10501 ttctggacac cattaaaaag gagaaattta acctcacggc caccatcggg gccggcacgc
    10561 tggtgaatca gggccagatg gaaaacggca tctggttctg gaaattccag tatccggtca
    10621 ggatgcgtct ggtcggtcag accagcaccc gccccgagca ggccttcacc tttgaaatca
    10681 ctgtccggcg cgttgaccca cgaatcaaac ccggcggcat ggaaatcagc cagatggttt
    10741 cccgtaacgc cgcatcagga acctgagact catatcatgc aaatacatca tcttcttggc
    10801 gcactgctta ttgtgtgcgg cactgtcacg gctgcagata acccgggctg gcagaacgca
    10861 cgaaccccac aggctggcgg aaagacagga caggctcccg ggaacgcccc
//
LOCUS       DAZKJR010000162        10900 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKJR010000162 DAZKJR010000000
VERSION     DAZKJR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.163"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(115..1602)
                     /gene="yeeO"
                     /locus_tag="ACHOWS_004386"
     CDS             complement(115..1602)
                     /gene="yeeO"
                     /locus_tag="ACHOWS_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFQ0049873.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVVMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1763..1838
                     /locus_tag="ACHOWS_004387"
     tRNA            1763..1838
                     /locus_tag="ACHOWS_004387"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1796..1798,aa:Asn,seq:gtt)
     gene            complement(1876..2826)
                     /gene="cbl"
                     /locus_tag="ACHOWS_004388"
     CDS             complement(1876..2826)
                     /gene="cbl"
                     /locus_tag="ACHOWS_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFQ0049874.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2928..3845)
                     /gene="nac"
                     /locus_tag="ACHOWS_004389"
     CDS             complement(2928..3845)
                     /gene="nac"
                     /locus_tag="ACHOWS_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFQ0049875.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4172..4247
                     /locus_tag="ACHOWS_004390"
     tRNA            4172..4247
                     /locus_tag="ACHOWS_004390"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4205..4207,aa:Asn,seq:gtt)
     gene            complement(4302..5237)
                     /gene="ldtA"
                     /locus_tag="ACHOWS_004391"
     CDS             complement(4302..5237)
                     /gene="ldtA"
                     /locus_tag="ACHOWS_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFQ0049876.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5299..6378)
                     /gene="cobT"
                     /locus_tag="ACHOWS_004392"
     CDS             complement(5299..6378)
                     /gene="cobT"
                     /locus_tag="ACHOWS_004392"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFQ0049877.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAILVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPISGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPRDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6390..7133)
                     /gene="cobS"
                     /locus_tag="ACHOWS_004393"
     CDS             complement(6390..7133)
                     /gene="cobS"
                     /locus_tag="ACHOWS_004393"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFQ0049878.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVVIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7130..7675)
                     /gene="cobU"
                     /locus_tag="ACHOWS_004394"
     CDS             complement(7130..7675)
                     /gene="cobU"
                     /locus_tag="ACHOWS_004394"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFQ0049879.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7809..8246)
                     /locus_tag="ACHOWS_004395"
     CDS             complement(7809..8246)
                     /locus_tag="ACHOWS_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFQ0049880.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLNGGSVQSLSDAYSDYSVLLLIAAESEISVPEWLPHSEGTTVIVIAEHNARLLTGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(8464..8639)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8728..9642)
                     /gene="pocR"
                     /locus_tag="ACHOWS_004396"
     CDS             complement(8728..9642)
                     /gene="pocR"
                     /locus_tag="ACHOWS_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFQ0049881.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(9845..10654)
                     /gene="pduF"
                     /locus_tag="ACHOWS_004397"
     CDS             complement(9845..10654)
                     /gene="pduF"
                     /locus_tag="ACHOWS_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFQ0049882.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVTGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
BASE COUNT         3070 a         2889 c         2318 g         2623 t
ORIGIN      
        1 tgaactacag aggaatcgtg agaacgaggc gaatattagc gatgcccatc cacaatgtca
       61 aagcctgttt tttaaatttg aaatcgtttg ctgaaataat ctgcattttg tcgtttattc
      121 cgacacaact ggcttttttt cacacttttg cggctcgggt cgagggtatt tccacagcca
      181 acgtccagta accattcgcc agtaaaacag cacggcccgc acagcccagt cggcaaacat
      241 tcccatccag acaccaacca caccccagcc aagcatgatt cccagcacat aaccgactac
      301 aacccggcaa ccccacatgc tcaacataga aacccacatg gcgtaacgtg catcgcgagc
      361 ccccttaaat ccagcgggta gcacccatga ggcggaccaa ataggcataa ataaagcatt
      421 tagccaaatc agaatcacaa cgacgtgttt aacctgtgga tcctgggtgt aaaacgatgc
      481 cataaccccg gcaaagggag ccgttagcca ggcgatggcc gttaatccaa gagtggacag
      541 ccagaacaca tgccgcaact gaatctctgc ttgcgctatc tgccctactc ccaaccttcg
      601 gcctgtaatg atcgtagagg cagaaccgag cgcacttccg ggtaagttga taagagccgc
      661 aattgagaac gcgataaaat ttccggcaat aacgctggtc cccatcccgg caacgaacat
      721 ttgagttaat aaccgaccac tggtaaataa cactgattcg acactcgctg gaataccaat
      781 ccccatgact tcccagataa tgctaaaatt cagcggttta aaatagctct ttaacgaaat
      841 ccttagcgca ggattaaaac caatcgccag cacccacaaa attgcaactg cgccaatata
      901 acgagaaatg gttaaaccca acccagcccc gacaaatccc agtcccggcc aggagaaaag
      961 gccgtaaatc aatatgccgc taataataat attaagaata ttcaggctac cgttaatcag
     1021 tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaataa gagtgatggc
     1081 agctgctgga taactgagta cggtcagctc cagataagtc aacgccagtg ctttaacttc
     1141 tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat gaattagcgt
     1201 tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc
     1261 tcgtcgtcga tctcgcttac cgagactaaa tgctacaacg acagtagtac caagatcgat
     1321 agctgcaaaa aaagccataa cgaccatatt gaagctgtcc gccaacccca cgccggccat
     1381 cgcatctttt cccagccagc tgaccagaaa agtgctcagg acccccatca acaggacaca
     1441 ggcgttctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga acaacacttt
     1501 gtagctcttg cgtttagagt gccagcgagt gccgtgaaca acctggcgta aagcagagga
     1561 gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaatg atgagggaga
     1621 atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga
     1681 tagaaacggc aaacagttgg gaaatttaaa aatcgggttt gacaaaatat ttttcgccgt
     1741 taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     1801 tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa gcccgctttt
     1861 atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt ccatcacctg
     1921 gcgcttgata tcctcaactg acagtcctgc attacaaagt tccagaaagc gccagacata
     1981 gttacgctga agttgccctc gcttcagtcc caaccagaca gtattagcat cgaaaagatg
     2041 ccgcgtatcc aggcggatta aattcttttc ctcttgttcg ccgctggatt gttcggcaac
     2101 taatccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgtgcact
     2161 taatacaata tctgccggca aacctttgcg ggcaaatgcg tcatcaatgc gtgagcgccc
     2221 cgtaattccc tgtcggtaag taattaacgg ccacttcgct attgattcca gcgtcaatgg
     2281 tgtaatttgc gttaagggat gatcgagtgg aacaagcaaa ctatggtgcc agcgaaacca
     2341 cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc
     2401 ttcgccattt tgcaacaacg tcgcaatttc ctgtggcgta ccctggatta gctcgagccg
     2461 aacctccggg aaaagttcgc gaaaagcttt aatgacctct ggcaagctat aacgtgcctg
     2521 agtatgcgtc gttgcaatag tgagaacacc agacgtatcg ttggtaaaca ggtctgcaag
     2581 ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta atgctttgcc
     2641 cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc
     2701 atcctccagt tcccgaatat gacggctaac gcctgactgt gaggtaaaaa gcatattcgc
     2761 aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg attatcttta gttgttggaa
     2821 attcacggta aactccgggc agttcagata tcccgttatt gttaaagtct aatgcccggc
     2881 ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta gctcaccaat
     2941 tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa ctctttcact
     3001 gcctgtgcct gtggtgacaa gtttgctctg gcgggtaaat ttaaggacaa agagagactc
     3061 attgaaggag tggtaatgcg tgacatccac ccatttactg cgccacataa cgaacgcgca
     3121 gccgattcag gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca
     3181 atagactcaa tttcaccaat gacttttgcc gtgagccgcc gtagggaaaa agcctcatca
     3241 acacgaagtc taacagcgct gtaatcactg gggagaaaaa ggttcatttg cgcaatagca
     3301 ttaacatcaa cgctttgccc cgggcaatct tgagttccta ccaggaaaag atcttctttc
     3361 agcaaagcct gactggatac cccagccaca ggggaatgct cataaatcac cgccatatcg
     3421 agttggtgat ttatcagttt ttcgttaagc actgcaccac tattttcatg aagatagata
     3481 acgacctccg ggaattcagc gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca
     3541 gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgactgacc aacgttatgc
     3601 accgccagtt gggcctgctc acactgacgt aaaatggccc gtgcatgggt atagagaatt
     3661 tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagctg ttgatttaac
     3721 tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca
     3781 gcagcctggg tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag
     3841 ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc gccattactt acaaccagaa
     3901 tgcaagatgc ttgccagttc tattttagcg ttgatgcgca agctaaccaa ctgtcaaata
     3961 agagattatg atagattcgt cattggccct ttttatcagc agtcgcattc acctgcccta
     4021 taaaaggagg gtatgcacca cgatggttca ttacccaata agattgaaag ctcaccactt
     4081 tgttgaaatt ggcagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca
     4141 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     4201 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     4261 ctgcttcgag caggcttttt gctttctaat taccaacgct cttaaaacat ctgtcttgaa
     4321 ccagaactaa tttgcacagg cattcccgat cgatgttgca acgcagcatt tgcgcgattt
     4381 acatcaactt cttgcccgtt gataaacgcc cgcaaagatg gggttaccgg caatggcact
     4441 tttcggtcag actcatattc tgcacgattg cgcgacagcg gctcatgaac ttccagccac
     4501 tttgaaccat ccggttccgt tgtgtatttc actggctgat caataatttg tacacgagtg
     4561 cctacaggaa cattatcaaa cagatatttg atatcgtcat tgcgcagacg aatacagccc
     4621 tgacttaccc ggagcccaat accaaaattg gcattggtac cgtggatggc atacaacctg
     4681 ccaatataaa tcgcgtacag ccccatgggg ttatcgggcc ccgcaggaac aaatgcgggc
     4741 aaactctccc ctcgtttcgc atattcgcgc cgagtgttcg gcgttggcgt ccaggttggt
     4801 gcttcttgtt tacgttcaac ggtagtcacc cagttacgcg gggtttctcg cccagcctgg
     4861 ccgataccaa taggaaagac ttccactgta ttactgtctg gcggatagta ataaagacgc
     4921 atttcagcga cgttaaccac aatcccttta cggacagtgg cgggcaaaat cagttgctgc
     4981 ggaatggtga gttgtgagcc agacttcggc aaaaaaacat cagcgcccgg attcgcttcc
     5041 agcatattac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgagtattg
     5101 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     5161 ggataagtta ccgccaggct attttggctg gcaaaaagca gagcaaatga gcaaagaata
     5221 tttacacgac gcatcatgtc cctttcctaa gtcgcgaata ctatccgata agtatagctt
     5281 ttatcagact tttcgttttt agctgttcaa atcagaagtc gtattccccg gtagaacaat
     5341 attactggca gcaagttcgc ccatattgtt gtatatcgca caagcagctt cgatgatggg
     5401 catcgccaga gctgcaccac tcccctcacc taaacgcata tccatattga gataaggctc
     5461 cagccccaaa tgcgacagcg ctatacgcgc gcctttttct gccgacaagt gagaaggaat
     5521 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcagcag cataagaaag
     5581 aaatccatcc agcaacacgg gtaaaccgca ggaagcagca cctaacatca ctccagctat
     5641 tccgaccaaa tcaaatccac ccacttttgc caggacatca acaccatccc gaggatttgg
     5701 ttgattcaac gtaatcgccc gacgcacaac atcaatttta ttagccagtt tatctgtcgg
     5761 caggtttgcg ccaatcccaa ccacttcttc aggatcccgg ccagtgattg tgctgactat
     5821 tgccgctgcc ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca gcgtgacacc
     5881 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt cagcctgacg
     5941 gcgactcatt gccggagctg aagcaatatt gccgctacct cgtgcgacac gcatgttgat
     6001 aagcccggag ataggctcag cagtatcaat accaacatca attacgtgga cgttagcgcc
     6061 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgtat
     6121 ggctgtcact tcttttggag aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     6181 cataaccagt atcgcttttt tgcccacatg cggtatgcca ttcaaccccg gcattcctgc
     6241 cagttgtatg gcaagcgcct ccagctttcc caggctacca acaggtttga gtaacccgtc
     6301 aatatgccgt tgtgcacgcg acatagcggc aggatcgatg gctgggatcg tattcagtaa
     6361 atcggcaagt atttgcatgt cacgtctctt tatagcagtg ccagcaggaa aaccagttca
     6421 ccaagctcta tggctgctcc cagcgtatcg cccgtttgcc cgcccagcgt acgtttgaga
     6481 agctgaccga ggatgaaaat caccaccatc gtcaccacca tagcagccac accatgcata
     6541 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     6601 ccatcaattt tgccaatgaa cacattgcca agcccttctt cccgcgcata acgatgacga
     6661 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     6721 atcggttcgc cacgcagagt caactcgctt aacaccagaa tctttaccag taccacaaaa
     6781 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     6841 tcgcggctac gtgcagaaaa tacgccatca caggtatcag cgaggccgtc cagatggaat
     6901 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     6961 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaatcaaa
     7021 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     7081 ggtacaggca gacgcgtaat aaatgagagc atcgcccaaa ataatttact catttgattt
     7141 ttactccaat acccgaaacc accagccata cttcatttgc cgcagcggcc aactgctgat
     7201 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     7261 ttcccacttc gttagtcact aatacaacct ttgcggggca acgttggcag gcagcaatca
     7321 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataattccat tcatcagggt
     7381 ctttatcgcc gccataatca aacaacagat tagtcaccat tgtggtaacg cattccagca
     7441 acacagcctc attcgggtta atgtctgcat gaattaattc atcaagatgt tgccaacgct
     7501 ccactgtgcg ccagtgctcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     7561 catcatcaag gatttgcgag gtggcgatat acagaacctg tgaagagtcc ccaataagcg
     7621 cctctgcgtg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     7681 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     7741 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     7801 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     7861 cgacaaaatg cgtcattgac agcggtttct ctggcatgac agatttcatt cagataatgg
     7921 atcaatgcca cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     7981 acaattaccg tcgttccctc agaatgaggg agccattctg gtacggatat ctcgctttca
     8041 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataatga ttgcacagac
     8101 ccaccgttaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     8161 aatatttcag ccagatgttt cgccaggtcc acatctccag ataagaggca aaaaagagct
     8221 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttagtttc ctgtaatgtc
     8281 ggtctggttg atgagccatg aaacaagatt gccacacctt aagtgcagcc ctggtgcctg
     8341 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     8401 ggggtataaa gacgcgccgg tactctaccg gacgcttccg ggcaacccac ttcccaccga
     8461 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc ccccgacctt
     8521 cccaatctcg cgatcagtgg tattttcggg tttgtcagca ttacagcagc gggggctgcg
     8581 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     8641 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     8701 aaaccttccc tttttttagc atttactcta taccgtttcc atcgccgcat tttcactcac
     8761 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     8821 actggtttgc gaaaaaccta aattacgcgc aatgctggcg atgctccagt cactatggca
     8881 cagtaactca cgagcgctgg ccatccgttg ttggtttacc cacgcattaa aaccgattcc
     8941 atggtatttc ttgaataact tactgaaata ataaggactt agataaacgt gcgaggcgac
     9001 atcttccaga cgcaaatcgt ctgataaatg cgcatcaata tagcgtaaag cttttttcat
     9061 tttattgtcg tggggattag gcgcacgagt ctggcgcacc gaatctgact gagatttgtt
     9121 ctctttaata accacaaaat tgagttgttt tttgaggcaa ttttccacaa tcaacttcaa
     9181 caaatcagca gaggccataa ctcttgagta atccatctca ggaactttgc ggaattcatt
     9241 aagtaattcg ggatctgcct gccagcaatc atcgacattc agaatatcga ccaaatcgac
     9301 atcatcatta cgcagacgaa cttgcccgca caatacaaaa ccaactaaat gtccggcgat
     9361 taccagtgga atagagaaat cggttaatcc ggcatgacag cgataaatac aaggctgatc
     9421 ggatttagag gcttccagac ccccacaacg atcgctcatg cgacatcgag tgctcagctg
     9481 agggtgttgc cgcatcatct gacaaaatgg agtgaagtta aacagctcgg aaatttcatc
     9541 gccgtgaata ttgaccacga caaccgcaag gctggtcgcc tgcgcaaaat cctgcgcgat
     9601 tttattaatg agttctgaat taagtgtcgc cgcagaaatc atgataaaac ctcccagtta
     9661 atttattgtt atagcaaaac aattgtttta catttaatct tcacatcatc cttgatgatt
     9721 tactgacgag aagcacaaag gacaataaca aaagcctccg tgaagcggga gactttttaa
     9781 taagttcgga aagcagatca caaacagtaa aaaaataaac gtttataact gccagattat
     9841 ttcgttattc gctgatttct ttaggcgtta cactattgac aggtaaattc tttgcaatga
     9901 gatagcgata aatcgccgca ccaacacatg ccccaatgat cggtgcaata atgggtacaa
     9961 tgaaataagg aatattgcgt ccaccagtca tcgcaatttc cccccaaccc gcaaaccagg
    10021 tgaaaatttt aggtccaaaa tcacgcgctg gattcatggc aaaaccggtt aatggtccgg
    10081 tagaagcacc aataacagca accagaatac caatgagtaa aggcgcaagc ggtcctttgg
    10141 gtacgccatt accgtcatcg gtcaacgcca taatcaaccc cattaacatg gacgttatga
    10201 ccacttcgac caacgctgct tgccatacat taatcgccgc tgctggataa gtactgaaga
    10261 tactggcgag ttgtaaactt tctgcgctac cacgcactat attgtgagca gattcaaaat
    10321 cggtaaacaa actgctgtag agagaatatg ccagaaccgc accaccaaag gcacccgcta
    10381 attgcgccac acaaaatggc agcactttac gggctgggaa agaggcaaac agccacagag
    10441 caatggtaac tgccgggttg agatgagcgc cggaaatacc tgccgtaagg taaacagcaa
    10501 gcgaaatccc taatccccaa atgatacaaa tttcccacag tcccagactt gccccggtaa
    10561 ccttcagggc gcacaaacaa ccaatgccaa aaaagaggaa cagtccggtg cccaaaaact
    10621 cggcaacgca ttgcgccttg agtgaatcat tcatagtgac accttcttta cggagttttt
    10681 ctttatcagc caactatgcg ctgattgttg tgtataccga gcgcaactat aaaaaattgc
    10741 tctgcagaaa atattgccaa catgcatgac agactcgtgt aaaaatcgtg ctaacataac
    10801 aaattattgt cattgccgaa tcatgcaaca cagcgttaat cagtgttgaa agtaataatt
    10861 tccaccaaac ttttacaata aaaaattgcc atcatgacat
//
LOCUS       DAZKJR010000163        10850 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKJR010000163 DAZKJR010000000
VERSION     DAZKJR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.164"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            288..1319
                     /gene="galR"
                     /locus_tag="ACHOWS_004398"
     CDS             288..1319
                     /gene="galR"
                     /locus_tag="ACHOWS_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFQ0049883.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(1326..2588)
                     /gene="lysA"
                     /locus_tag="ACHOWS_004399"
     CDS             complement(1326..2588)
                     /gene="lysA"
                     /locus_tag="ACHOWS_004399"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0049884.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            2710..3645
                     /gene="lysR"
                     /locus_tag="ACHOWS_004400"
     CDS             2710..3645
                     /gene="lysR"
                     /locus_tag="ACHOWS_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFQ0049885.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(3632..4324)
                     /gene="ygeA"
                     /locus_tag="ACHOWS_004401"
     CDS             complement(3632..4324)
                     /gene="ygeA"
                     /locus_tag="ACHOWS_004401"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFQ0049886.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADKRAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(4453..5871)
                     /gene="araE"
                     /locus_tag="ACHOWS_004402"
     CDS             complement(4453..5871)
                     /gene="araE"
                     /locus_tag="ACHOWS_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFQ0049887.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(6186..6947)
                     /gene="kduD"
                     /locus_tag="ACHOWS_004403"
     CDS             complement(6186..6947)
                     /gene="kduD"
                     /locus_tag="ACHOWS_004403"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFQ0049888.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(6977..7813)
                     /gene="kduI"
                     /locus_tag="ACHOWS_004404"
     CDS             complement(6977..7813)
                     /gene="kduI"
                     /locus_tag="ACHOWS_004404"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0049889.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGIINIGGAGTITVDGQCYEIGHRE
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(8100..9281)
                     /gene="yqeF"
                     /locus_tag="ACHOWS_004405"
     CDS             complement(8100..9281)
                     /gene="yqeF"
                     /locus_tag="ACHOWS_004405"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFQ0049890.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            9536..10765
                     /gene="yqeG"
                     /locus_tag="ACHOWS_004406"
     CDS             9536..10765
                     /gene="yqeG"
                     /locus_tag="ACHOWS_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0049891.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         2799 a         2863 c         2499 g         2689 t
ORIGIN      
        1 cctgcgcatc tgcgcaggct ggtgtaattc atgtgctcaa cccgaagttg acttcaccta
       61 tcaatacctc tgggatcacc actttagcaa cctgaagcca aacgccacca gcggtcaatc
      121 gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc tctttttttg tcttacttat
      181 ttgcgtttgg ctcacattcc cacaatgaaa acacgccgcc ccttgaacca acgggcgttt
      241 tccgtaacac tgaaagaatg taagcgttta cccactaagg tattttcatg gcgaccataa
      301 aggatgtagc ccgactggca ggcgtttcag tcgccaccgt ttcccgcgtc attaataatt
      361 cacccaaagc cagcgaagct tcccggcttg ctgtgcatag tgcaatggag tctcttagct
      421 atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac cactgaaacg gtcggtctgg
      481 tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt gaaagcggtc gaacaggtgg
      541 cttatcacac tggtaatttt ttattgattg gcaacggtta ccacaacgaa caaaaagagc
      601 gtcaggccat tgagcaactg atccgccatc gctgtgctgc gttggtcgtc catgccaaaa
      661 tgatcccgga tgccgattta gcctcattaa tgaaacaaat gcccggtatg gtgctgatca
      721 accgtatcct gcctggcttt gaaaaccgtt gtattgctct ggacgatcgt tacggtgcct
      781 ggctggctac gcgtcattta attcagcaag gtcatacccg cattggttat ctgtgctcta
      841 accactctat ttctgacgcc gaagatcgtc tgcaagggta ttacgatgcc cttgctgaaa
      901 gtggtattcc ggccaatgac cggctggtga catttggcga accagacgaa agcggcggcg
      961 aacaggcaat gaccgagctt ttgggacgag gcagaaattt cactgcggta gcctgttata
     1021 acgattcaat ggcggcgggc gcgatgggcg tgctcaatga taatggtatt gatgtaccgg
     1081 gtgagatttc gttaattggc tttgatgatg tgctggtgtc acgctatgtg cgcccgcgtc
     1141 tgaccaccgt gcgttaccca atcgtgacga tggcgaccca ggctgccgaa ctggctttgg
     1201 cgctggcgga taatcgccct ctcccggaaa tcactaatgt ctttagtccg acgctggtac
     1261 gtcgccattc agtgtcaact ccgtcgctgg aggcaagtca tcatgcaacc agcgactaac
     1321 cgcagttaaa gcaattccag cgccagtaat tcttcaatgg tctggcgacg gcgaatcaac
     1381 cgcgcctgac cattatcaaa cagaacttct ggtaacagcg gacggctgtt gtagttggat
     1441 gacattgatg cgccatatgc ccctgtatca tgcagtacca gataatcacc tgccttcact
     1501 tccggcaagg cgcgggtttc gacatttccc ccttcctgct gggtaaagac atcgcccgat
     1561 tcacataacg gcccggcgac gacggtttcc accgttggtg cgtgttccag agaacgacca
     1621 tcagctgcca gggcactgat atggtggtaa ctaccgtaca ttgccgggcg catcagatcg
     1681 ttgaacccgg catcaaccag cacaaagtgg cggctaccca tttgtttgac gctccgcact
     1741 tgagtaatta atacgccaga ctgcgctacc agaaagcgac ccggttcaat ttccagtttc
     1801 acagggtggc ccaaatggcg ggcgatttgc tgacgcgcgg cattccacag accataataa
     1861 tgctcggtat caaccgcctc ttcaccctgt tgataaggaa cagaaagccc accgcccgca
     1921 gaaatagcct gtaaatcctg accgaattcg aggacctgac gcaccatagc accacatacc
     1981 tgttccagat gggcataatc gacgccagaa ccaatgtgca tgtgaatgcc gaccagctgt
     2041 agatgatgac gttgtatcac gtccagtgcg gcgggcagat cggtgtacca gataccgtgc
     2101 ttgctgtttt cgccaccggt attggttttt tggctatgcc cgtgaccaaa ccccggatta
     2161 acacgcagcc atacccgatg ccctggcgaa acctgaccca gttggtcgag catatcaaca
     2221 gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt caagcgtcgc ctgatcgata
     2281 acatctgccg taaaaacaat atcatcaggg tgcgtttgcg gattgtaacc cgccgccagt
     2341 gcacgctcta tttcgcctaa cgagacggaa tccactttca cgccctgctc acgcattaag
     2401 cgcaaaatat gaatattgga acaggctttc tgtgcgaagc gcaccacatc aaactgtttc
     2461 agcgctgcaa tctgccgacg aataatttgc gcatcgtaga cccacaccgg gcagccaaat
     2521 tctgcgggca aacgcagcag attttcggcg gtgagatcgg tatcagtgct gaacagtgaa
     2581 tgtggcataa caaactccag ataagtgctt ttttatgatt acgccacatc ataaaaagaa
     2641 taaaaaatat cgatttatgt cgagtctatg caaaaatgat atggattacc ggattgcgag
     2701 agagcgctaa tggccgccgt taacttacgt catattgaaa tttttcatgc ggtaatgacc
     2761 gccggaagcc tgactgaggc ggcacacctg ctacacacct cacagccaac tgtcagccgc
     2821 gaactggcgc gctttgagaa ggtgatcggg ctgaaattgt ttgagcgcgt acgtggacga
     2881 ttgcatccta ccgtgcaagg actgcgtctg tttgaagaag tgcaacgatc ctggtacgga
     2941 ctggatcgca ttgtcagcgc cgcagaaagt ctgcgcgagt ttcgccaggg agaactgtct
     3001 attgcctgcc tgccggtctt ttcgcaatct tttttaccgc agctcctgca accctttctg
     3061 gcacgttatc ccgatgtcag cttaaatatc gtgccccagg aatcaccgct acttgaagag
     3121 tggctctcgg cccagcgtca tgatttagga ctcacggaga cactccatac gcctgcggga
     3181 acagaacgta ccgaattact ctctttagat gaagtgtgtg tgttacctcc gggccatccg
     3241 ctggcggtaa aaaaggtatt aacgccggat gattttcacg gtgaaaacta catcagcctt
     3301 tcccgtactg acagctatcg ccagttgctg gatcaattgt ttactgagaa tcaggttaaa
     3361 cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtct gcgcaatggt acgggcgggg
     3421 gtaggtgttt cggtggttaa cccgctcacc gcactggatt atgcggcaag cggtttagtg
     3481 gtacggcggt tcagtattgc ggttccgttc accgttagcc tgatccgccc cctgcaccgc
     3541 ccgtcatcag cgctggttca ggcgtttagt gagcatttac aagcgggatt accgaaactg
     3601 gtcacttctc ttgacgctat tttgtcgtca gctacgacag cataaaagcg acagcatcct
     3661 cggcatggat cgccgcagta tcaaacacag gcagaacact gcgctcttct ggcaccagta
     3721 aaccaatttc cgtacagcca aaaatgacac cctgtgcgcc ctgttctgca aggcgagcaa
     3781 tcacttgcgc ataataagtg cgtgacgctt cggtaaattg ccccagacac agttcttcaa
     3841 aaataatctg attaattttc gcccgtttat ccgcttcagg aatgagacag ttgatggaaa
     3901 attgttccgt cagccgcccg cgataaaaat cctgttccat ggtgtaacgc gtacccagca
     3961 gcgccacacg agtcattcca gcccggataa ttgcacgtcc agtggcatcc gctatgtgta
     4021 agaaaggcag agagcaacga gactcaatgg catccgccac tttatgcatc gtattggtgc
     4081 ataacacaat accttctgcg cccgcccgct gtaagccaag cgccgcctca gccagaatat
     4141 ccccggtttt gtcccactca ccgcgacgct ggcactcttc tatttcatga aaatcgacgc
     4201 tatgtagcag cacttgcgca gagtgaagcc caccaagccg ctgtttaatg ccttcattta
     4261 tcagacgata gtaaggaata gtggattccc agctcattcc tcccagcaaa ccaattgttt
     4321 tcattcctct ctcctgatgt gtgttaccgc aacaaggctg aataaggaac gagacaaacg
     4381 cctcaacggc caggtgccta atctctatta acgaaaaaag ggccggatgt acagcacatc
     4441 cggcccgtga aatcagacgc cgatatttct caacttttcg cctgccatca gtttgcgctc
     4501 gatatgttcc agcgtgacat ttttggtttc cggaatgagc cagaaagtga tgcccacaaa
     4561 cgcaatgttc agcgcagtgt agagccagaa cgtaccggca gcgccaatgc tatcaagcag
     4621 tgtcaggaag gtcgcgccga taatcatatt cgacacccag tttgtcgtcg ttgagcaggt
     4681 gatgccaaaa tcgcggcatt tcagcggctg aatttcagag cacaagatcc acaccactgg
     4741 cgcggcgctc atcgcataac cggcaataca catcatcgtc atgccaacag agagccagga
     4801 caagccactg gaagccgtac cgttatcaaa ctgcatcagg cagtaaccca gcaccagagt
     4861 gcctaacgcc atcacgctga aaccaatttt cagagccggc ttacgccctg ctttatctac
     4921 cgtaaagacc gcaatgaagg tagcgaacat aaaggtcagc cccacgacca gagtcgcaat
     4981 catctgttgt tctgtggtcg taaagcccgc cattttgaag atacgtggcg cgtagtacat
     5041 gatgatgttc ataccggtaa actgctgcat cgcctgcaac aacataccga gaaacacagc
     5101 acgacggacg ttacggttga tcttaaacag tgcccaaccg ccctgtttta acttcaggct
     5161 ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac gtatcgcgca gcatacgcaa
     5221 cacttcttcc gcctcaatat gacgcccttt ttccgccagc cagcgcgggc tatttggcag
     5281 gaagaccacc agaataatca gcaggactgc tggtaaagca agaaccccca acattgcgcg
     5341 ccagttaccg ctataactga acgctgtatc ggataaaaac gccagcacga tgccgagtgt
     5401 gaccatcaac tggtacatac tgatcatctt accgcgaacg ttttcacttg ccatttcaga
     5461 aagatacaga ggagcggtgt aagacgcgat cccgacagca atgcccagca ccacacgagc
     5521 ggcgattaac atctctacgc tggtcgcaaa tgcggaccct atcgaaccga gcacaaaaag
     5581 gatagccccc gccatcaggc tgtatttacg ccccaggcgg aacgacagcc acccattaaa
     5641 cagcgcacca attgctgcgc cgagcatcat gctactgacc acccattcct gcaaacggct
     5701 ggtcagcaca aagtgatcgg taatgaacgg caacgctccg gcgattacgc cgatatcaag
     5761 accaaataac aatcctgcga ccgcagcagc taccgaaaca aacatattca tacgccgcgt
     5821 atcccgcaaa gaacgtggcg ttaaagcaga ttccgtattg atagtaacca ttttttcctg
     5881 ccagcagaga gtaagacata gtgaaaaaat acgtgaacaa ttcacacagg tgtcaggtcg
     5941 gaaatagcat aaatatggat taaattgctg cgacatgtcg ttatgtgatg gatattccaa
     6001 ttttcaaatt aagttgaatt attgagatta ttattaacca cctaatttta aagcagataa
     6061 aattcataaa gttcattaat tgataattga tatggattat ttcataacca tgatatggat
     6121 tatgatgatc tacaggtata aaaaaccctg ccatgcggca gggtcataaa agtaagagaa
     6181 atgaattaac gagccagcca accgccatcc acggcaatgg tataaccatt cacataatct
     6241 gaagcgctgg aggcaaggaa cactaccggc cccatcaggt cactcggcag tccccaacgg
     6301 ccagctggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg tagttgttga
     6361 gtattgttgg ttgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc
     6421 cattcattcg ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc ggtataagaa
     6481 ggcacacgga tcccgccctg gaaggagagc attgacgcga tattgataat cttgccgcca
     6541 ttgccttgcg cgataaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata
     6601 ttcaggttca tgacatcgtc ccagtctttt tcgctgaact cgagagcgtc ttcgcggcga
     6661 atcaatccgg cgttattcac caggatatca atatgaccaa actcagctac cgcgcgatcc
     6721 agcagtgctg gaatgccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc
     6781 cccagcgccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat gccaacaatg
     6841 tcacagcctg cttgcgccag ccccaacgcc atcccctggc ccagcccagt atcacaacca
     6901 gtgacgaccg caactttacc ttcgagagaa aatgcactta aaatcataac aataccttat
     6961 tcgtttatgc ccacaactag cgcaaatctt taacggccac atggtccata tcatcaaaaa
     7021 cctggttttc accgaccatg ccccagataa aggtataagc tttggttccg acgccggaat
     7081 ggatcgacca gctcggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
     7141 cttgcggttg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
     7201 aaacttccat ccggcgctcg tgggtgtgac acggcatggt gttccacaag ttacctggag
     7261 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
     7321 tgttaatcgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
     7381 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
     7441 tcgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
     7501 acagggcttc gcggtgaccg atttcatagc attgaccatc gacagtaatc gtaccggcac
     7561 cgccaatatt gataatacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
     7621 taccaacttc cccgccaacg gaaaccgttt tagttaccgg cataatgccg ccaacaataa
     7681 tacggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
     7741 aaaattcatt gcgcagcccc tgggtatcca gcgtttttgc gtgcgcactg tggatgctct
     7801 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
     7861 aaacgctgtt tcgttttctc tataagggga tgatatgccc acgggatagg gttttcaatt
     7921 aaaaataaaa caatgtttca tttttaaggt aaggactgaa aaaagtgcag tgtcgatcac
     7981 gaatatgtca tgagaatgga aaaatcaggg agccctgaaa aggtatgtcg actcgccagc
     8041 caccattatt caacagatta atgacggctg atatttatcg gtaaggagga aatgcagagc
     8101 tattcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgtagcc
     8161 agtcctttgc gggcattacg tttcaccatt tcatgaacca gagaaacgag gattcggcaa
     8221 ccggaagcgc ctattgggtg accgagtgcg atcgcgccac cattgacatt gacacgacgc
     8281 tcatcccatt caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
     8341 tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
     8401 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
     8461 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattactgca
     8521 gctgcgccat cgtttatgga tgatgcatta cccgctgtca cagaaccgag actatcaaat
     8581 gaaggattta aacgggctaa gccttctgca ctggcgtcag tgcgtggctg ctcatcggta
     8641 tcaacaacca acgtctgacc gttactttgg gtcattaccg ggacgatctc atctttaaat
     8701 cgtccagcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
     8761 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
     8821 tcattgaagg catcccacaa cccatcgtgc acaagactat caaccaactg gctattgcca
     8881 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
     8941 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
     9001 gctttaagcc cggaaccgca aacgtcatta atagtgattg cagaaacgct attaggaaga
     9061 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
     9121 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
     9181 acgaccaggc tgccaagttc cacggcggaa tgacccgcta acgcaccacg gaagcagccg
     9241 atgggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
     9301 acagtgcaat agtaaatgaa tgttaattac agttatctta attgtttaaa aaaagtgatt
     9361 tttatcacaa aggaaatatg cctgagtagc aatcagagac ataactggca cgtaaggttt
     9421 gcaaccacta acccaccaat agagaggtag aaagggcgtt aatctcccat acttaacctg
     9481 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
     9541 taatatttgg tcaaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
     9601 tgcaggcacg ctcttccttc ctattcagtt aggttcggcg ggggctgtgg tcctgtttat
     9661 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
     9721 cctctcatcg aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg
     9781 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
     9841 gatatatgca gtggcaatta ccaactcact tacagaacag ctggcaaagc atatggttat
     9901 tgatcttcgc atccgtatgt tggtgagtct gggggttgta ttaatcctga atctcatttt
     9961 tctgatggga cgccatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
    10021 ctatttctta tttctttcta tttacctggt cggtagttgg caacctgatc tattaacaac
    10081 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
    10141 tttcgctttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
    10201 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
    10261 cataagtgta ttgttctttg tctttagctg cctgctttct attccacctt cgtatattga
    10321 agcggctaaa gaagaagggg ttaccatttt atcggcgctt tctatgctgc cgaacgcccc
    10381 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
    10441 gggtacgtac tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa cattacagca
    10501 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
    10561 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag
    10621 cggcccgctc attgccatga tacttttcat catgcctacg ctgtcaacgt atctcatccc
    10681 ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacc ctgatcgtgg gtatcctgtg
    10741 cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt gcttcaattt
    10801 tgaaccatcc cagttttcat aatgttcatt tgttattatt ccgctctatc
//
LOCUS       DAZKJR010000164        10716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKJR010000164 DAZKJR010000000
VERSION     DAZKJR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.165"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(14..178)
                     /locus_tag="ACHOWS_004407"
     CDS             complement(14..178)
                     /locus_tag="ACHOWS_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049892.1"
                     /translation="MHTPIGVKPVAGSKEWQEAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLIRI"
     gene            287..781
                     /gene="napF"
                     /locus_tag="ACHOWS_004408"
     CDS             287..781
                     /gene="napF"
                     /locus_tag="ACHOWS_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFQ0049893.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNACGACAASCPVSAITAEY
                     LHVH"
     gene            771..1034
                     /gene="napD"
                     /locus_tag="ACHOWS_004409"
     CDS             771..1034
                     /gene="napD"
                     /locus_tag="ACHOWS_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFQ0049894.1"
                     /translation="MYTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1031..3517
                     /gene="napA"
                     /locus_tag="ACHOWS_004410"
     CDS             1031..3517
                     /gene="napA"
                     /locus_tag="ACHOWS_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFQ0049895.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3524..4219
                     /gene="napG"
                     /locus_tag="ACHOWS_004411"
     CDS             3524..4219
                     /gene="napG"
                     /locus_tag="ACHOWS_004411"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFQ0049896.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4206..5069
                     /gene="napH"
                     /locus_tag="ACHOWS_004412"
     CDS             4206..5069
                     /gene="napH"
                     /locus_tag="ACHOWS_004412"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFQ0049897.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPASVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5066..5515
                     /gene="napB"
                     /locus_tag="ACHOWS_004413"
     CDS             5066..5515
                     /gene="napB"
                     /locus_tag="ACHOWS_004413"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFQ0049898.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5525..6127
                     /gene="napC"
                     /locus_tag="ACHOWS_004414"
     CDS             5525..6127
                     /gene="napC"
                     /locus_tag="ACHOWS_004414"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFQ0049899.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6140..6763
                     /gene="ccmA"
                     /locus_tag="ACHOWS_004415"
     CDS             6140..6763
                     /gene="ccmA"
                     /locus_tag="ACHOWS_004415"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFQ0049900.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAENKIRRISLTQTRGE"
     gene            6760..7422
                     /gene="ccmB"
                     /locus_tag="ACHOWS_004416"
     CDS             6760..7422
                     /gene="ccmB"
                     /locus_tag="ACHOWS_004416"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFQ0049901.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7464..8201
                     /gene="ccmC"
                     /locus_tag="ACHOWS_004417"
     CDS             7464..8201
                     /gene="ccmC"
                     /locus_tag="ACHOWS_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFQ0049902.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8198..8407
                     /gene="ccmD"
                     /locus_tag="ACHOWS_004418"
     CDS             8198..8407
                     /gene="ccmD"
                     /locus_tag="ACHOWS_004418"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFQ0049903.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8404..8883
                     /gene="ccmE"
                     /locus_tag="ACHOWS_004419"
     CDS             8404..8883
                     /gene="ccmE"
                     /locus_tag="ACHOWS_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFQ0049904.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8880..>10716
                     /gene="ccmF"
                     /locus_tag="ACHOWS_004420"
     CDS             8880..>10716
                     /gene="ccmF"
                     /locus_tag="ACHOWS_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFQ0049905.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAMLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRNGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRW"
BASE COUNT         2203 a         2738 c         3210 g         2565 t
ORIGIN      
        1 tgctctttta gtgttaaatt ctgatgagaa aacaaaccag cagaaatatc tctggcgaat
       61 taatcgctat ataaatatgt ttataaccat ttgaaatgtg agcaaaagcc cgtttttgcc
      121 acgcttcctg ccactctttt gatcctgcta caggttttac cccgatcggg gtatgcatct
      181 ttgacacatc ctttaatatc ttagcggcta taaaaatggc ttattaatta tgcggtttat
      241 ttggtcgctc tcaattttca gagcgcgtta atgatggaag gtcaatgtga agattgatgc
      301 atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc agtaacggta tccgtccgcc
      361 ctggtccggt gatgaatctc attttctgac ccattgcact cgctgcgacg cctgtattaa
      421 tgcttgtgaa aacaacattc tgcaacgcgg cgcgggtggc tatccgagcg ttaatttcaa
      481 aaataacgag tgcagcttct gttacgcctg cgcgcaggct tgccccgaat cgctattttc
      541 tccgcgccac accagggcct gggatttgct gttcaccatc ggggaggcgt gcctggcgta
      601 tcagtcagtt gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa ttatctttcg
      661 cccgacgctc tccgggatct accagccgca acttaatagc caactttgca acgcatgtgg
      721 agcatgtgca gccagctgtc cggtatcagc cataactgcg gagtatctcc atgtacacta
      781 actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca gacatcagca
      841 cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg agcggccagt
      901 tgattgtggt ggtggaagca gaagacagcg aaacgctgat ccaaaccatt gagtcagtac
      961 gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa gagcaaggtg
     1021 aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg ttgcggccgc
     1081 tgcggcggct gccggtctca gcgtgccggg cgttgcccgc gccgttgttg gtcagcagga
     1141 agccattaaa tgggataaag cgccgtgccg tttctgcggt acgggttgcg gcgttctggt
     1201 cggaacgcag caggggcgtg tggtggcctg tcagggcgac ccggacgcac cggttaaccg
     1261 tggtctgaac tgcattaagg gctatttcct gcccaaaatc atgtacggta aagaccgttt
     1321 gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg aatttacccc
     1381 aatcacctgg gatcaagcct tcgatgtgat ggaagagaaa ttcaaaaccg ccctgaaaga
     1441 aaaagggccg gaatcgatcg gtatgttcgg ttctggtcag tggactatct gggaaggtta
     1501 tgccgcgtcc aagctgttta aagcgggatt ccgttcgaac aacatcgacc cgaacgcgcg
     1561 tcactgtatg gcgtcggcgg tagttggctt tatgcgtacc tttggtatgg atgagccgat
     1621 gggctgctat gacgacatcg agcaggctga cgcgtttgtg ctgtggggct ccaacatggc
     1681 ggagatgcac ccgatcctct ggtcgcgcat cactaaccgt cgtctctcca accagaacgt
     1741 caccgtggcg gtgctttcta cctaccagca tcgtagcttc gagctggcgg ataacggcat
     1801 catctttacg ccgcaatctg acctggtgat cctgaactat atcgccaact atatcattca
     1861 aaacaatgcg ataaatcagg acttcttcag caaacacgtt aacctgcgca aaggggcgac
     1921 ggacatcggc tacggtttac gtccgaccca tccgctggaa aaagcagcga agaatccggg
     1981 ttctgacgcc tccgaaccga tgagctttga agattacaaa gccttcgttg ctgaatatac
     2041 gctggaaaaa accgccgaaa tgaccggcgt gccgaaagac cagttagaac aactggcgca
     2101 gctgtatgcc gatccgaaca agaaagtcat ctcctactgg acgatgggct tcaaccagca
     2161 tacccgtggt gtgtgggcca acaacctggt ctacaacctg cacctgctga ccggcaaaat
     2221 ttcccagccg ggttgcggtc cgttctccct gaccgggcag ccttccgcgt gtggtactgc
     2281 gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac atggtggtga ctaacgagaa
     2341 acatcgtgat atttgcgaga agaagtggaa tatcccgagt ggcaccattc cggcgaaaat
     2401 cggtctgcac gcggtggcac aagaccgtgc gctgaaagac ggcaagctga atgtttactg
     2461 gaccatgtgt accaacaaca tgcaggccgg gccgaacatt aatgaagagc gtatgccagg
     2521 ctggcgcgat ccgcgcaact ttatcatcgt ttccgatccg tatccgacag tcagtgcgct
     2581 ggcagcggac ttgatcctgc cgaccgcaat gtgggtagag aaagaggggg cttacggtaa
     2641 cgccgaacgc cgtactcaat tctggcgtca gcaggtacag gcaccgggcg aagcgaaatc
     2701 ggatctctgg cagttagtcc agttctcccg ccgcttcaaa actgaagaag tatggccgga
     2761 agagctgctg gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag ttctgtatgc
     2821 caccccggaa gtgagcaaat tcccgctatc cgaactggcg gaagatcagc tgaacgatga
     2881 atcccgcgag ctgggcttct atctgcaaaa agggctgttc gaagagtacg catggtttgg
     2941 tcgcggtcac ggtcacgatc tcgcgccgtt cgatgactac cacaaagcac gcggtctgcg
     3001 ctggccggtg gtgaacggta aagaaacgca gtggcgttac agcgaaggta acgacccgta
     3061 cgtgaaagcg ggcgaaggct acaagttcta cggtaaaccg gatggcaaag cggtgatctt
     3121 cgcgctgccg ttcgaacctg cggcggaagc accggatgaa gagtacgacc tgtggctctc
     3181 taccggacgc gtcctggagc actggcatac cggcagtatg actcgccgtg taccggaact
     3241 gcaccgcgcc ttcccggaag cggtcctgtt tattcacccg ctggatgcga aagcgcgcga
     3301 tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt ggcgaagtga tctcgattgt
     3361 cgaaacgcgc ggtcgtaacc gtccgccgca gggcctggtg tacatgccgt tcttcgatgc
     3421 cgcacagctg gttaacaaac tgacgctgga tgcgaccgat ccgctctcga aagagacgga
     3481 cttcaagaag tgcgcggtca aactggagaa ggtgtaagcc attatgtccc ggtcagcgaa
     3541 acctcaaaat ggtcgccgcc gctttctgcg cgatgtcgtt cgcacagctg gcgggctggc
     3601 tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg gcgtgcggtt
     3661 gcgcccgccc ggagccataa acgagaacgc ctttgccagt gcctgtgtgc gttgcggtca
     3721 gtgtgttcag gcttgccctt acgacacctt aaaactggcg acgctggcct ctggcctgtc
     3781 ggcgggcacg ccatattttg tcgcacggga tattccttgc gaaatgtgtg aggacattcc
     3841 gtgcgccaaa gtgtgcccga gcggtgcgct ggatcgtgag atcgaatcga tcgacgacgc
     3901 gcgaatgggg ctggcggtac tggtggacca ggaaaactgt ctcaactttc aggggctgcg
     3961 ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag gccatcaccc tggagctgga
     4021 gcgcaacacg cgtaccggta agcacgcccg ctttctgccg acggttcaca gcgacgcctg
     4081 taccggttgc ggtaagtgcg aaaaagtgtg cgtgctggaa caaccggcaa tcaaggtgtt
     4141 accgctgtca ctagcgaaag gggagttagg tcaccattac cgcttcggct ggctggaggg
     4201 gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc gctggagaaa aaaggctggt
     4261 ggcgcagtca ccgttggtta gtgttacgtc gcctttgtca gttcttcgtg ctggggatgt
     4321 ttttgagcgg tccgtggttt ggtgtgtgga tcttgcacgg caactacagc agtagcctgt
     4381 tattcgacac cgttccgctg accgatccgc tgatgacgct gcaaagtctt gccagcggtc
     4441 atctgccggc ctcggtggcg ctgaccggag cggtgattat caccgtgctc tacgccctgg
     4501 caggaaagcg attattttgc agctgggtct gcccgctgaa cccgattacc gacttagcga
     4561 actggttacg caggcgcttt gacctcaatc agtctgcgac gatcccacgc cacattcgct
     4621 acgtgctgtt ggtggtcatt ctgctgggat cggccttaac cggcacgctc atctgggaat
     4681 ggatcaaccc cgtttccctg atggggcgca gtctggtaat gggattcggc agcggcgcgc
     4741 tgcttattct cgcgctgttt ttatttgatc tactggtcgt tgaacacggc tggtgcgggc
     4801 acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg attaccgttg
     4861 cggccaccga tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt tgcccggaac
     4921 cgcatgtgct acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc accagccgcg
     4981 attgcatgac gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt acaataacta
     5041 cacgatggag ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag cgctgtgtca
     5101 atggacggcg atgctggccc tggtggtgag cggcgcggtt tgggcggcta atggcgtcga
     5161 ttttagccag tcgccggaag tatccgggac gcaggaaggg gccattcgga tgccaaaaga
     5221 gcaggatcgg atgccgctga actatgtgaa tcagccgcca atgatcccgc atagcgttga
     5281 aggttatcag gtaacgacca ataccaaccg ctgcttgcag tgccacggtg tcgaaagcta
     5341 tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt atggacagcg acggcaaagt
     5401 gggcgcagaa gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac cgcaggccga
     5461 taccgcgcca atcgtgggga atacctttac cccatcaaaa ggttacggga aataagaggt
     5521 tattatggga aattctgacc gtaagcctgg tctgattaag cgcctgtgga aatggtggcg
     5581 tacccccagc cgtctggcgc tggggacgct gctgttgatc ggttttgttg gcggcatcgt
     5641 cttctggggc ggctttaaca ccgggatgga aaaagccaat accgaagagt tctgcattag
     5701 ctgccacgaa atgcgtaaca cggtgtatca ggaatacatg gattccgtgc actacaacaa
     5761 ccgtagcggc gttcgtgcga cctgtccgga ttgtcacgtt ccgcacgagt ttgtgccgaa
     5821 gatgatacgc aagctcaaag caagtaaaga gctgtatggt aaaatttttg gcgttattga
     5881 cacgccgcag aaatttgaag ctcatcgcct gacgatggca cagaatgagt ggcggcgcat
     5941 gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata tggatacaac
     6001 cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg ggcaaacctg
     6061 tattgattgc cataaaggga tagcgcacaa gctgcccgat atgcgcgaag tcgagccagg
     6121 tttttaacag ggttattgcg tgggtatgct tgaagccaga gagttacttt gcgagcggga
     6181 tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac gcaggagagt gggtacaaat
     6241 caccggtagc aacggcgcgg ggaagacaac gcttctccgt ttgctgacgg ggttgtctcg
     6301 ccctgatgcg ggtgacgttc tctggcaagg gcagcccttg catcaggtac gcgacagcta
     6361 ccatcaaaac ctgttatgga tagggcatca gccggggatc aaaacccggc tgacggcgtt
     6421 agaaaatctg cacttttatc atcgcgatgg cgataccgca caatgtctgg aagccctggc
     6481 gcaggccggg cttgccggat tcgaagatat tccggtaaat caactttctg ccgggcaaca
     6541 acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc acgttatgga tcctcgacga
     6601 gccttttacc gcgattgacg ttaacggcgt cgatcgtctg acccagcgta tggcgcagca
     6661 tacggagcag ggggggattg tgattctgac tacccaccag ccgctcaacg ttgctgaaaa
     6721 taaaattcgc cgcatttcac tgacgcaaac gaggggcgaa taatgttctg gcgcattttc
     6781 cgtcttgagc tgcgcgtagc gtttcgccat agcgccgaaa tcgccaaccc gctgtggttc
     6841 ttcctgattg tcattaccct ttttccgctc agtatcggtc cggagccgca actgctggcg
     6901 cgtattgcgc cgggcattat ctgggttgct gcgctgcttt catccttgct ggcactggag
     6961 cgactgttcc gtgacgattt gcaggacggc agtcttgaac aattgatgtt gttgccgtta
     7021 cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac cggtctgccg
     7081 ttactcatcc tttcgcccct ggtagcaatg ctactgggaa tggatgttta tggctggcaa
     7141 gtgatggcgt taacgctact gctgggaacc cctacgcttg gctttcttgg tgcgccgggc
     7201 gtggcgctga cagtgggact taagcgcggt ggtgtgctgc tcagcatact ggtgttaccg
     7261 ctgactatcc cattactcat ctttgccacc gccgcgatgg acgcggcttc tatgcatttg
     7321 cccgttgatg ggtatctggc aattttaggc gcgttgctgg caggcaccgc gacattaagt
     7381 ccttttgcga cggcggcagc gttacgaatc agcattcaat aacgctggat tactgatttt
     7441 tgttcgagtc tggtatcgaa actatgtgga aaacactgca tcaactggcg atcccaccac
     7501 ggctgtatca aatctgtggc tggtttatac cgtggctggc aattgccagt gtggtcgtgc
     7561 ttaccgtcgg ctggatctgg ggattcggtt ttgctccggc tgattatcag cagggaaata
     7621 gctaccgcat tatctacctg catgtgcctg cggcgatctg gtcgatgggc atttatgcat
     7681 caatggcggt ggcggcgttt attggccttg tctggcagat gaaaatggcc aacctggcgg
     7741 tggcggcgat ggcccccatt ggtgccgtgt ttacctttat tgccctggtt accggctctg
     7801 catggggaaa accgatgtgg ggcacctggt gggtatggga tgcacgcctg acttctgaac
     7861 tggtactgct gtttttgtat gtgggtgtga ttgccctgtg gcacgccttc gacgaccgcc
     7921 gtctggcggg ccgtgcggca ggtattctgg tgctgattgg cgtggtgaat ctgccgatta
     7981 ttcattactc cgtggagtgg tggaacaccc tgcatcaggg atcaacgcgg atgcaacaaa
     8041 gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc ctgctgctgt
     8101 ctgccacgct gacgctgatg cggatgcgta atttgatttt gctgatggaa aaacgccgtc
     8161 cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg acccctgcat ttgcttcctg
     8221 gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg tggtgatgac
     8281 cgttattccg ctggtgattt tggtcgtgca ctcggtgatg cagcatcgcg caattctgcg
     8341 tggcgtggcg caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac agcaggaggc
     8401 tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgct ggcggggctg
     8461 gcgctgacta tcggtctggt gctatatgcg ctgcgctcga atatcgatct cttttatacg
     8521 ccgggggaga ttctctacgg caagcgtgaa actcagcaga tgccggaagt cggtcagcgt
     8581 ctgcgcgttg gcgggatggt gatgccgggg agtgtgcagc gcgatcccaa ttcgctaaaa
     8641 gtaactttca ccatttacga cgctgaaggt tcagtagatg tctcttacga aggcattttg
     8701 ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg gcgagctgga aaaaggcaat
     8761 catatcctcg cgaaagaagt gctggcgaaa cacgatgaaa actacacgcc gccagaagtt
     8821 gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg tttataagga cccagcatca
     8881 tgatgccaga aattggtaac gggctgctgt gtctggcgct aggaattgcg ctgctgctgt
     8941 ctgtgtatcc gctatggggc gtggcgcgcg gggacgcgcg catgatggcg tcttcccgct
     9001 tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt tctggtgctg gtcaatgctt
     9061 tcgtggtcaa cgactttacc gtcacctatg ttgccagcaa ctccaatacc cagcttccgg
     9121 tgtggtatcg cgtggcggct acctggggcg cgcatgaagg ctcgctcctg ctgtgggtgc
     9181 tactgatgag cggctggacc tttgcggtag cgatttttag tcagcgtatt ccgctggata
     9241 ttgtggcccg cgtactggcg ataatgggga tggtcagtgt cggctttttg ctgttcattc
     9301 tctttacctc taacccgttc tcacgcacgc tgccgaattt cccgattgaa gggcgcgatc
     9361 ttaacccgct gttgcaggat ccggggctga tcttccatcc gcctctgctc tatatggggt
     9421 acgtcggttt ctcggtggcg tttgctttcg ccattgcttc tttgttgagt gggcgtctgg
     9481 acagcaccta tgcgcgtttt acccgcccgt ggacgctggc ggcgtggatc ttcttgacgc
     9541 tcggcattgt gctcggttcc gcatgggcct attacgaact cggctggggc ggctggtggt
     9601 tctgggatcc ggtagaaaac gcctcgttta tgccgtggct ggtggggact gcgctgatgc
     9661 actcactggc ggtcactgaa caacgcgcca gcttcaaagc gtggacatta ctgctggcaa
     9721 tcagtgcctt ctcgttgtgt ctgttgggga ctttcctcgt gcgttccggc gtgctggtat
     9781 cggtacacgc gtttgcgtct gatccggcgc gcggtatgtt tatcctcgcc tttatggtgt
     9841 tggtgattgg cggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt cgctcacgcg
     9901 taaacaatgc gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt ttgctggtcg
     9961 ccgcgatgct ggtggtatta ctggggacgc tgctgccgct ggtgcacaag caactgggac
    10021 tgggcagtat ttcgattggc gaaccgttct tcaacaccat gtttacctgg ctgatggtgc
    10081 cgtttgcgct gctgcttggt gtcggtcctc tggtgcgctg ggggcgcgat cgcccacgta
    10141 aaatccgcaa tttattgatt atcgccttca tctcaacgct ggtgctgtcg ctgctgctgc
    10201 cgtggctgtt cgaaagcaaa gttgtggcga tgacggtgct cggcctggca atggcctgct
    10261 ggattgcgat gctggcaatt gcggaagctg cgctgcgtat ttcacgtggc acgaaaacca
    10321 ccttcagtta ttggggaatg gtggcggctc acctggggct ggcagtgaca attgttggta
    10381 ttgcctttag ccagaactat agcgttgagc gtgatgtgcg catgaagtcc ggcgatagcg
    10441 tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact ggtccgaact
    10501 ggcgtggcgg tgtggcgact atcggcgtaa cgcgaaatgg caagccggaa acagtgctgt
    10561 atgcggaaaa acgttattac aacactgccg ggtcgatgat gaccgaagcg gcaattgacg
    10621 gcggcatcac gcgtgacctg tacgcggccc tcggtgaaga gctggaaaac ggcgcgtggg
    10681 ccgtgcgtct ttactacaaa ccgtttgttc gctgga
//
LOCUS       DAZKJR010000165        10695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKJR010000165 DAZKJR010000000
VERSION     DAZKJR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.166"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            125..1807
                     /gene="ydeN"
                     /locus_tag="ACHOWS_004421"
     CDS             125..1807
                     /gene="ydeN"
                     /locus_tag="ACHOWS_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFQ0049906.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEKLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1859..3016
                     /gene="ydeM"
                     /locus_tag="ACHOWS_004422"
     CDS             1859..3016
                     /gene="ydeM"
                     /locus_tag="ACHOWS_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFQ0049907.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHTENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETISYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2982..3104)
                     /locus_tag="ACHOWS_004423"
     CDS             complement(2982..3104)
                     /locus_tag="ACHOWS_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049908.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
     gene            3307..4992
                     /gene="yddA"
                     /locus_tag="ACHOWS_004424"
     CDS             3307..4992
                     /gene="yddA"
                     /locus_tag="ACHOWS_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0049909.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAEYLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVI"
     gene            5030..7402
                     /gene="yddB"
                     /locus_tag="ACHOWS_004425"
     CDS             5030..7402
                     /gene="yddB"
                     /locus_tag="ACHOWS_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFQ0049910.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDFTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDVNPQ
                     IVFSYIKSKGNLSLNNGYEESNTDDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYINEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7459..10242
                     /gene="pqqL"
                     /locus_tag="ACHOWS_004426"
     CDS             7459..10242
                     /gene="pqqL"
                     /locus_tag="ACHOWS_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0049911.1"
                     /translation="MRNLCFLLMLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPSNKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNAQDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLLPLITLYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10604..>10695
                     /locus_tag="ACHOWS_004427"
     CDS             10604..>10695
                     /locus_tag="ACHOWS_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049912.1"
                     /translation="MDKKLVTDLRSELLDSRFGAKSISTIAESKR"
BASE COUNT         3154 a         2349 c         2317 g         2875 t
ORIGIN      
        1 gactgttatt tctctttttt tagcttaatc tgctatctct ttatgtgact aacttcactt
       61 acatccactt atttctcttc gtaaaattac tttggaatta agtacaataa gaagaggaac
      121 atttatgaag tctgcattaa agaaaagtgt cgtaagtacc tcgatatctt tgatactggc
      181 atctggtatg gctgcatttg ctgctcatgc ggcagatgat gtaaagctga aagcaaccaa
      241 aacaaacgtt gctttctcag actttacgcc gactgaatac agtaccaagg gaaagccaaa
      301 tattatcgta ctgactatgg atgatcttgg ttatggacaa cttccttttg ataagggatc
      361 ttttgaccca aaaacaatgg aaaatcgtga agttgtcgat acctacaaaa tagggataga
      421 taaagccatt gaagctgcgc aaaaatcaac gccgacgctc ctgtcattaa tggatgaagg
      481 cgtacgtttt actaacggct atgtggcgca cggtgtttcc ggcccctccc gcgccgcaat
      541 aatgaccggt cgagcgcccg cccgctttgg tgtctattcc aataccgatg ctcaggatgg
      601 tattccgcta acagaaactt tcctgcctga attattccag aatcatggtt attacactgc
      661 agcagtaggt aaatggcact tgtcaaaaat cagtaatgtg ccggtaccgg aagataaaca
      721 aacgcgtgac tatcatgaca acttcaccac ctattctgcg gaagaatggc aaccgcaaaa
      781 ccgtggcttt gattacttta tgggattcca cgctgcagga acggcatatt acaactcccc
      841 ttcactgttc aaaaaccgtg aacgcgtccc cgcaaaaggt tatatcagcg atcagttaac
      901 cgatgaggca attggcgttg ttgatcgtgc caaaacactt gaccagcctt ttatgcttta
      961 cctggcttat aatgctccgc atctgccaaa tgataatcct gcaccggagc aatatcagaa
     1021 gcaatttaat accggtagtc aaacagcaga taactactac gcttccgttt attctgttga
     1081 tcagggtgta aaacgcattc tcgaaaaact gaagaaaaac ggacagtatg acaatactat
     1141 tattctcttt acctccgata atggtgcggt tatcgatggt cctctgccgc tgaacggggc
     1201 gcaaaaaggc tataagagtc agacctatcc tggcggtact cacaccccaa tgtttatgtg
     1261 gtggaaagga aaacttcaac ccggtaatta tgacaagctg atttccgcaa tggatttcta
     1321 cccgacagct cttgatgcag ccgatatcag cattccaaaa gaccttaagc tggatggcgt
     1381 ttccttgctg ccctggttgc aagataagaa acaaggcgag ccacataaaa atctgacctg
     1441 gataacctct tattctcact ggtttgatga ggaaaatatt ccattctggg ataattacca
     1501 caaatttgtc cgccatcagt cagacgatta cccgcataac cccaacaccg aggacttaag
     1561 ccaattctct tatacggtga gaaataacga ttattcgctt gtctatacag tagaaaataa
     1621 tcagttaggt ctctacaaac tgacggatct acagcaaaaa gataaccttg ccgccgccaa
     1681 tccgcaggtc gttaaagaga tgcaaggcgt ggtaagagag tttatcgaca acagccagcc
     1741 accgcttagc gaggtaaatc aggagaagtt taacaatatc aagaaagcac taagcgaagc
     1801 gaaataacta aaccttcatg cggcggattt ttccgccgcc ttattgagcg agatagcgat
     1861 gcacgttaca gccaagccct ccagttttca atgtaatctc aaatgtgatt actgttttta
     1921 ccttgaaaaa gagtcgcagt ttactcatga aaaatggatg gatgacagca ctctgaaaga
     1981 gttcatcaaa caatatatcg cagcgtctgg caatcaggtc tattttacct ggcaaggcgg
     2041 tgaacccact ctggctggcc tggatttttt ccgtaaagtt attcactatc aacaacgcta
     2101 tgcaggccaa aaacgtattt ttaatgcatt acaaacgaat ggcattttat tgaataatga
     2161 atggtgtgcc tttctcaaag aacatgagtt tctggttggt atctcgatcg atggccccca
     2221 agagctacat gaccgttaca gacgcagtaa ttcaggtaac ggtacttttg caaaagtgat
     2281 agcagccatc gagcgtctga aatcatatca aatagagttt aatacgttaa ccgttattaa
     2341 taacattaat gtccattacc ctcttgaggt ttatcatttt ttaaaatcta tcggcagtaa
     2401 acatatgcaa tttatcgaat tgctcgaaac cgggacgccg aatattgatt tcagtggtca
     2461 tactgagaat acattccgta tcattgattt ttctgtgcct cccacggctt atggcaagtt
     2521 tatgtcaacc atttttatgc aatgggttaa aaacgatgtg ggtgaaattt tcatccgtca
     2581 gtttgaaagc tttgtcagcc gttttttggg gaatgggcat accagttgta ttttccagga
     2641 gtcctgcaag gataatctgg ttgttgaaag taatggagac atttacgaat gcgaccattt
     2701 tgtctatcca cagtacaaaa ttggaaacat taataaatcg gaactcaaaa cgatgaacag
     2761 tgtacaactg acagcgcaaa aaaaacggat ttcagcgaaa tgtcagcaat gtgtatataa
     2821 acctatctgc aatggtggtt gccctaagca tcgtattact aaagtaaaca atgagactat
     2881 ttcctatttt tgcgaaggtt ataaaatcct tttttcaacc atggtacctt atatgaacgc
     2941 catggtggag ttagccaaga acagagtacc gctttaccac attatggatg ttgcaaaaca
     3001 aatggagaat aattaataac gctccttctt tgctccagtc ttagcctccg tcttatgacc
     3061 ttaacaaagc acccgacata aattcctgtt gggtgtttcg tcatcatcat cttcccttgc
     3121 gaaaattctg gcttacgcgg catgcgtcag ggcattagca atacccttga ttatcaatag
     3181 cataaccata accactgaaa atgaaatatt tctttatatt tcatattgtt aaatgatact
     3241 taaataaatt tcatcagtaa atcattgcca tagataatca ttggatggca caatcaattt
     3301 tcgctaatga taaccattcc cattacgttt tgtatgttga tagcaaagta tctctgtctg
     3361 ctgaagccat tttggctgcg taagaataat aaaacttcag tgttgttaat cattattatc
     3421 ctcgccatga tccttggcgt ggttaaaatt caggtctggt taaacgactg gaataatgat
     3481 tttttcaatg cgctaagcca gaaagaaacc gataagcttt ggcagcttgt cctttggttc
     3541 cctgccctgc tggggatctt tgtattaata tccgtaaaca aaacatggct aattaaacta
     3601 ctgactattc gctggcgcga atggttaaca gattactacc ttaatcgctg gtttgcagat
     3661 aaaaactact acttcacgca gatctacggt gagcataaaa atacagataa ccctgaccaa
     3721 cgtatcgctg aggacattct cttactgatc agtaaaacgc tcagcctttc atttggcttc
     3781 atccagtcac ttagcatgtt gatcaccttt accgttattc tttggcaaag cgcgggtacg
     3841 ctctcattca ctgttggtgg aacagaatgg aatatccagg ggtatatggt ctataccgtt
     3901 gtgctcattg tgatcggtgg aactttattt acccataagg ttggtaaacg tattcgtccg
     3961 cttaatgtgg aaaaacaacg tagcgaagcg acatttcgaa ctaatcttgt gcagcataat
     4021 aagcaggcag aactgattgc gctaagcaat gctgagtatc tacaacgcca ggagttaagc
     4081 gacaattttc ataccattaa agagaactgg catcgtttaa tgaatcgtca gcggtggctt
     4141 gattactggc agaatattta ttcgcgctcg cttagcgttc ttccctactt tctgttattg
     4201 ccgcagttta ttagcggaca gatcaatctg ggcggactga tgaaatcgcg ccaggcattt
     4261 atgctggtat cgaacaattt aagctggttt atttataaat atgacgaact tgctgaactg
     4321 gctgcggtta tcgatcgttt gtatgagttc catcaactca ccgaacagcg ccctacgaat
     4381 aagcctaaaa attgccaaca tgcggtacaa gtggctgacg cgagtattcg tactcctgat
     4441 aataagatca tattagagaa cctgaacttt catgtttcgc caggcaaatg gctattactg
     4501 aaaggttact ctggtgcggg aaaaaccaca ctgcttaaaa cattatccca ctgctggccg
     4561 tggtttaaag gtgatatttc ttctcctgct gacagttggt atgtgtcaca aacaccgtta
     4621 atcaaaaccg gcttactgaa agagataatt tgtaaagcac ttcccctgtc cgtagacgat
     4681 aaatcgttga gcgaagtact gcatcaggtt ggtctgggga aattggctgc gcgtattcat
     4741 gaccacgatc gctggggcga tattctttcc agcggcgaaa aacaacgtat cgccctggca
     4801 cgattaattt tacgacgtcc gaaatggata tttcttgacg aaactacctc tcatcttgag
     4861 gaacaagagg ctatccgctt actgcgttta gtgcgtgaaa aactacccac aagcggcgtc
     4921 attatggtta cacatcaacc cggtgtctgg aacctggctg atgatatttg tgacattagc
     4981 gcggttatat aaaaacaata aaagccctgc tgtacggaga tataaataaa tgaagcgagt
     5041 tcttattcct ggcgtcattt tatgtggcgc tgatgtagcg caggccgtcg atgacaaaaa
     5101 catgtacatg catgtttttg aagagatgac ggtctatgct cctgtccctg tacccgtaaa
     5161 cggcaacacg cattacacca gtgaaagcat cgagcgttta ccgaccggga atggcaatat
     5221 cagcgatctg ctgagaacca accctgcggt gcgcatggat tcaacgcaaa gtacctcatt
     5281 gaaccaggga gatattcgcc cggagaaaat ctctattcac ggtgcgtcgc cctaccagaa
     5341 tgcctatttg attgatggta ttagtgcaac taataacctg aacccagcga atgagtccga
     5401 tgccagtagt gcaaccaata ttagcgggat gtcacagggc tattatcttg atgtcagctt
     5461 actggacaat gtgacgcttt atgacagttt tgtgccggtt gaattcggtc gcttcaatgg
     5521 cggggtaatt gatgcaaaga tcaaacgctt caacgctgat gatagcagcg tgaaactggg
     5581 ttatcgcact acgcgttcgg actggttaac atcgcatatc gatgagaata acaagagcgc
     5641 atttaatcaa ggttcttcag gaagtactta ttactctcca gattttaaaa agaactttta
     5701 taccttgtcg tttaatcagg aactcgctga taactttggc gttaccgccg gtttatcgcg
     5761 ccgccagtct gatatcaccc gcgcggatta tgtttcgaat gacggcattg tcgccggtcg
     5821 ggcacagtat aaaaacgtta tcgataccgc attgagcaaa tttacttggt ttgccagcga
     5881 ccgctttacc cacgatttta ccttaaaata taccggctcc agtcgtgatt ataataccag
     5941 caccttcccg cagtctgatc gcgaaatggg taataaatcc tacggtctgg catgggatat
     6001 ggatacgcag ctcgcatggg ccaaactacg tactaccgtt ggttgggatc atattagtga
     6061 ttatacccgt cacgatcatg acatctggta caccgaactt tcatgtacat atggtgatat
     6121 ttcaggtcgt tgcacccgtg gtggattagg acacatttcc caggctgtag ataattacac
     6181 cttcaaaaca cgcctggact ggcaaaaatt cgccgtgggt gatgtttcgc atcaacccta
     6241 cttcggcgcg gaatacatct attccgatgc atggactgaa cgccataacc agtctgaatc
     6301 ctatgtgatt aatgctgcag gaaagaaaac taaccatacc atttaccata aaggtaaagg
     6361 cagtctggga attgacaact acacgctgta tatggcggat cgcattagct ggcggaatgt
     6421 gtcgttaatg cccggtgtgc ggtatgacta tgacaactat ctgtcaaacc acaatatctc
     6481 cccgcgcttt atgacggaat gggatatttt tgctgatcaa acctcaatga ttaccgcagg
     6541 ttataaccgt tactatggcg ggaatattct tgatatggga ttacgtgata tccgcaatag
     6601 ctggacggaa tcggtatcag gcaataaaac cctgacgcgt tatcaggatt tgaaaacgcc
     6661 ttataacgat gaactggcaa tggggttgca gcagaaaatc ggtaagaacg ttattgcgcg
     6721 cgcaaactat gtttaccgtg aagcgcatga tcaaatcagc aaaagcagtc gtaccgacag
     6781 cgcgactaaa accaccatta ctgaatataa caacgacggc aaaaccaaaa cgcattcgtt
     6841 caacctcagt tttgaactgg ccgaacccct gcatatcagc caggtagatg ttaacccgca
     6901 aattgtcttt agctatatca agagcaaggg caacttgtcg ttaaacaatg gttatgagga
     6961 aagcaatacc gatgataacc gggtggttta taacggtaat ctggtctctt acgatagcgt
     7021 tccagtggcg gattttaata atccattaaa gatctcctta aacatggatt tcacgcatca
     7081 accgagcggg ttggtgtggg cgaatacgct gacctggcaa gaagcgcgta aagctcgcat
     7141 tatcctgggt aagacgaatg cgcaatacat caacgaatat tcagattaca agcagtatgt
     7201 tgacgagaaa ctggatagca gcctgacctg ggacacccgc ttgtcctgga cgccacaatt
     7261 tctgaaacaa caaaacctga cgatcagtgc cgatattctc aatgtactgg atagcaaaac
     7321 cgcggttgat acaacgaata ccggcgtggc gacctacgcc agtggccgta ctttctggct
     7381 tgatgtcagc atgaaatttt aatagttcgg attttacttt accgggtaaa tttgcccggt
     7441 tttcgcatgg agataattat gagaaacctc tgtttcttac tgatgttagt ggcaactctg
     7501 ttgctccccg ggcgactgat tgccgccgcc ttaccgcagg atgaaaagtt aattaccggg
     7561 caactggaca atggcttgcg atatatgatt tatccgcatg ctcaaccaaa ggatcaggta
     7621 aatttatggc tgcaaattca taccggttca ttgcaggaag aggacaatga gcgcggcgtg
     7681 gctcattttg tagaacatat gatgtttaac ggcacaaaaa catggccggg taataaagtc
     7741 atcgaaacat ttgagtcaat gggcctgcgt tttggtcgcg atgttaatgc ctataccagc
     7801 tatgacgaaa cagtgtatca ggtgagtttg ccgactacgc agaaacaaaa tctgcaacaa
     7861 gtgatggcaa tcttcagtga atggagtaat gccgcaacct ttgaaaaact cgaagtagac
     7921 gctgaacgcg gcgtaattac tgaggaatgg cgtgcccatc aggatgcgaa atggcgcacc
     7981 tctcaggcgc gccgcccttt cctgctggca aatacccgta atttagaccg tgaacctatc
     8041 ggcctgatgg atactgtcgc tacggtcaca ccggcacaat tgcgccaatt ttatcaacgc
     8101 tggtatcaac caaataatat gacctttatc gtggtcggcg atattgacag taaagaagcg
     8161 ctggcgctga ttaaggataa tttaagtaag cttccgtcta acaaagcagc tgaaaatcgc
     8221 gtctggccga caaaagccga aaaccacctg cgctttaata tcatcaatga taaagaaaac
     8281 cgggtgaacg gcatcgcact ctattatcgc ctgccaatgg tacaagtgaa cgatgagcaa
     8341 agctttatcg aacaagctga atggagcatg ttggttcagc tgttcaacca acgtctgcaa
     8401 gaacgcatac agtcgggcga gttgaagact atttctggcg gcactgcgcg cagcgtcaaa
     8461 attgcacccg attatcagtc tttgtttttc cgtgtaaatg cacaagacga taatatgcag
     8521 gatgctgcga atgcattaat ggcagagttg gcaaccattg atcagcatgg cttttctgct
     8581 gaagaactcg atgatgttaa atctacccgc cttacctggc tgaaaaatgc ggttgatcag
     8641 caagctgagc gtgatttacg tatgctgacc agtcgcctgg catccagctc attaaataat
     8701 acgccgttct tgtcgccgga agagacatat caactttcga aacgtctgtg gcagcaaatt
     8761 accgtgcaaa gtctggcgga aaaatggcag cagttaagaa agaaccagga cgcattttgg
     8821 gagcaaatgg taaacaatga agttgccgcc aaaaaagcat tgtctcctgc ggctatcctg
     8881 gcgctggaaa aagagtacgc caacaaaaag ctggcggctt acatcttccc aggcagaaat
     8941 ttatcgttaa cagtagacgc tgacccacag gcggaaatta gcagcaaaga aacgctggct
     9001 gagaatctga catcattaac actttccaat ggtgccaggg ttattctggc aaaatccgcg
     9061 ggtgaagagc aaaagctaca aattactgcc gtatctaata aaggcgattt aagtttccct
     9121 gcgcagcaaa aatcacttat cgcgctggca aataaagcag taagcggaag cggcgttggt
     9181 gaactctcct cttccagcct gaaacgctgg agtgcggaaa actcggtaac catgagcagt
     9241 aaagtcagtg gcatgaatac attgctctct gttagcgcgc ggactaataa ccctgaacct
     9301 ggtttccagt tgattaacca gcgaatcacc cacagcacga ttaacgataa tatttgggca
     9361 tcgctacaaa atgctcaaat tcaggcgttg aaaacgctcg accagcgtcc agcggagaaa
     9421 ttcgcccagc agatgtatga gacgcgctat gctgatgacc gcacgaaatt actgcaagaa
     9481 aatcagattg cacagtttac tgccgcagat gcgctggctg ccgatcgcca gttgttttca
     9541 tctccagcgg atatcacgtt tgtcattgtc ggtaatgtcg cagaagacaa actcctgccg
     9601 ttaattacgc tttacttagg atcaatcaaa cactctgatt cgccattagc cgcaggtaaa
     9661 ccattaactc gcgcgacgga caacgcatcg gttactgtaa aagaacaaaa tgaacctgtg
     9721 gcacaggttt cacagtggaa gcgttatgat tcccggacac cggttaatct ggagacgcgt
     9781 atggcgctcg atgcttttaa cgtcgcactg gcaaaagatc tacgcgttaa tattcgtgaa
     9841 caggcctctg gggcatacag cgtttcttct cgcctctcgg ttgatcctca ggccaaagat
     9901 atcagtcatt tgctggcttt tacttgtcaa ccggaacgac atgatgaact gttaacgtta
     9961 gcgaatgaag tgatggttaa gcgtctggct aaagggatca gtgagcaaga actgaacgaa
    10021 taccagcaaa acgttcagcg cagcctcgat atccaacagc gtagcgttca acaattagcg
    10081 aacaccattg taaatagtct tattcaatat gacgatcctg cagcatggac tgagcaggag
    10141 caattattga aacaaatgac ggtagagaat gttaacactg ccgttaaaca atatctttct
    10201 catccggtga atacctatac cggagtatta ttgccaaaat aataacagcc ccgtcaacac
    10261 atcgttggcg gggattttat caatattcgc tatttttatg taataatttt ataaatgcgt
    10321 tcaaaataat aaccaagtac taatagtgat attttaaggt ctgattttta cgtgataatt
    10381 caggaggcgt agaaagtgca taaaaataac aacacaaaac accttgctac cacccaataa
    10441 ttcaataatg tattgatttt caatgaaaaa atattattcg cataatatct cacgataaat
    10501 aacattagga ttttgttatt taaacacgag tcctttgcac ttgcttactt tatcgataaa
    10561 tcctactttt ttaatgcgat ccaatcattt taaggagttt aaaatggata agaagctagt
    10621 aacggattta aggtcggaac tactcgattc acgttttggt gcgaagtcta tttccactat
    10681 cgcagaatca aaacg
//
LOCUS       DAZKJR010000166        10637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKJR010000166 DAZKJR010000000
VERSION     DAZKJR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.167"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="ACHOWS_004428"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="ACHOWS_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFQ0049913.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1606..1929)
                     /gene="yfiM"
                     /locus_tag="ACHOWS_004429"
     CDS             complement(1606..1929)
                     /gene="yfiM"
                     /locus_tag="ACHOWS_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFQ0049914.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1973..3328)
                     /gene="pssA"
                     /locus_tag="ACHOWS_004430"
     CDS             complement(1973..3328)
                     /gene="pssA"
                     /locus_tag="ACHOWS_004430"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFQ0049915.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWILITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3442..6102)
                     /gene="pat"
                     /locus_tag="ACHOWS_004431"
     CDS             complement(3442..6102)
                     /gene="pat"
                     /locus_tag="ACHOWS_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFQ0049916.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSKINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6134..6832)
                     /gene="tapT"
                     /locus_tag="ACHOWS_004432"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6134..6832)
                     /gene="tapT"
                     /locus_tag="ACHOWS_004432"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFQ0049917.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6901..7320)
                     /gene="trxC"
                     /locus_tag="ACHOWS_004433"
     CDS             complement(6901..7320)
                     /gene="trxC"
                     /locus_tag="ACHOWS_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFQ0049918.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7527..8564
                     /gene="yfiF"
                     /locus_tag="ACHOWS_004434"
     CDS             7527..8564
                     /gene="yfiF"
                     /locus_tag="ACHOWS_004434"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFQ0049919.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRC
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVLQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8612..9301)
                     /gene="ung"
                     /locus_tag="ACHOWS_004435"
     CDS             complement(8612..9301)
                     /gene="ung"
                     /locus_tag="ACHOWS_004435"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0049920.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREDVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            9606..9989
                     /gene="grcA"
                     /locus_tag="ACHOWS_004436"
     CDS             9606..9989
                     /gene="grcA"
                     /locus_tag="ACHOWS_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFQ0049921.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10045..10632)
                     /gene="eamB"
                     /locus_tag="ACHOWS_004437"
     CDS             complement(10045..10632)
                     /gene="eamB"
                     /locus_tag="ACHOWS_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFQ0049922.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2627 a         2686 c         2754 g         2570 t
ORIGIN      
        1 aggctatccg tcaggatggc ctttttgcgc tggagcagaa taaatgccgg atgcgacgct
       61 ggcgcgtctt atactcccac ataagctaga ttcagcagcg aatacggctt ccccaacgtg
      121 cccacctcca tacgtgtcct ccttaccaga aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcgt ggttaaggtt gcataatgat atgcaacaaa tgtataacat
      241 tttctttacc aatagaaata aacaaaagcg accgacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc cttccgggaa
      481 cacgacgact caactactgc aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgct cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcgggcaac tgctagctct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcacttagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 gttagatgaa acttcacaac aagaaacacg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 acttattggc gcgctgtcgg ataagattgg tcgtcgtacc tcaatgttat gtttcggttc
     1261 tctggcagcc atttttaccg ttcctattct ctcagcattg cagaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgcgccct gctgatagtg agtttttata cttcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gccaatgcta tatttggtgg gtcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcatt
     1561 tctggtttca ctaacattgc accgtaaagg aaaagggatg cggctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgatatcc caggcaaaat ccttccagct
     1681 ccagccgctc ccctcagggc ggctatccca aagctctttt gacgccccca gactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgcatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaacaaac cccgtacagg acggggttgt gattacagga
     1981 tgcggctaat taatcggtcg atgcggatac ggcgcaaacg acggatgagt ttacgaacct
     2041 tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg gtggtatgct
     2101 cgcggatcag ctccagttct ttctctcgct gtggcgcaag ctccagttgc ggatcgtgga
     2161 tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta ttaccggtga
     2221 tcaatatcca cttatcatca acccacatcc cttttaggtg ataggtgttg tcgtcatctt
     2281 tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg ctcaggaaac
     2341 gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa ggttcatctt
     2401 ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc tttttccctt
     2461 cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag tatggcgtac
     2521 agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg ttcaacagac
     2581 tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg gcatcgccct
     2641 ggaaatgata agcggcatca cgcagctcct ggcggaacag gcgaatgtcg ttcttgattt
     2701 ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca ttcataatat
     2761 tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatt agatgataac
     2821 ggtcgtagcg atatttatcg tgctgatgca ggtaaacatc gttcaggctg gcaccgctat
     2881 aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaagg gcttcacggg
     2941 tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc gccatgcggc
     3001 agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt tgtgcacgat
     3061 gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc tcatacaacg
     3121 cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcaaca atgcaaatgc
     3181 gctgcttcgc gctggctatt ttttccagca acgtctcccg gaagtcggcg ggagcgtaaa
     3241 agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg tgttgttgat
     3301 gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt cattgaaggg
     3361 tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc gattaagtgg
     3421 tcaacatttc cagtacctta ctcatgattc ctcgcgctgg gcaagattta gcgtaagccc
     3481 aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg ccagcgccac
     3541 catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt gatctcgcgt
     3601 ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga gatccgagcg
     3661 aaccagcacg gcaaattcgg catcgatgtt atcagggtcg gagatcgcac gcgtgacgcc
     3721 gaggatctct tctgtttgat caatacgtcg taccgccaca aaagccattt cccgatcgta
     3781 gtcgatctgc gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct tgctaaagta
     3841 gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagct gtggctcatc
     3901 ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta cccattcttc
     3961 cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt caaacggcgc
     4021 gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca aaggatgaat
     4081 atccagacgc tgaatttccg ggcaatcgac aatcaagtta gaaacctgca ccagaagctg
     4141 gctcaagcct gcaacatcta atgggcgtag cgcactgcgc gcacgaatct ttttactttt
     4201 gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggta gtgcgacgac
     4261 ggcttgatct tcaggacgcc actccacacc gccttcgccc agcatgatca acggcccgaa
     4321 aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt tagccatact
     4381 ttgcaccaac aggccgtgga tccgcgcctg cggccaggcc atttttacgc gatcgaaaat
     4441 agcgtttgcc gcttgctgga cttcattagc tgtacgcagg tagagcatta cgccctgaac
     4501 ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat aaccaatctg
     4561 ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggtgggga gcgtgttcat
     4621 gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag ccccttcggc
     4681 aatcgcctgt tgcaacagaa gatgcgcttc tgcggtatta gaggtcagat tgctgggcaa
     4741 cgccggcgtt tcgcgtagtt gcttctgatt acgtcggtac tccaccatat gcataaaagc
     4801 agtgatggtt ccttccggcg tgcgataggt cggcagcccg gcttcactga ataatcgtcg
     4861 tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc agagaaacat acttgctgcg
     4921 gggatgatgc tttaccgctt caattaatgc ttgcgcactt tctgttgcgg gagcagcggc
     4981 gctgggcgaa tgaataacca tcagcgcgtc aaaatcctgg ctgtggagca gaatatccag
     5041 cgttttaata tagtgctcac tgctggcgtc atcgcgtaga tcgagcgggt tagagattgc
     5101 cacatgttct ggcagtgcat cgcgcagttt ctggcaggtt tcttcgctta gcgttgccag
     5161 cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag caccgttgct
     5221 gataatcatc agccggtcgc cacgcagcgg gcgcatatgg ctgagggttt ccaccgccga
     5281 aaacagctcg tgagtatcct gtacccgcaa caaaccggca cgctgaatag ccgcatccca
     5341 cgccggatcc attcctgccg tggtgttcaa cagacgttga gccgccgggc tacgtccgct
     5401 tttaatcacc agaatcggtt tgttgcgaga ggcactacgg gccgccgaaa caaagcgtcg
     5461 tgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt cgcgggccag
     5521 atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa taaagtagga
     5581 aaagcccatc tcacgctgtt gcgcccagtc gaggatggta ttggagacgg cagccgattg
     5641 cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg cattcagacc
     5701 ttgccaggga gccagtaacc ccagactgtt tggtcctagc aggcgcatgt tatggcgcag
     5761 ggcgcaggcg cggagatctt catgttgcga tgccggggcg gaaagaataa tgcaggtttt
     5821 acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat tggtacataa
     5881 aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca acacacccaa
     5941 caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc ccgccagcag
     6001 gttacgcatc atcaggtaac ctgctcgatt ggatttcatc gatgcgccaa ttaccgcaat
     6061 cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct cccgcagaag
     6121 tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc agatgttgcg
     6181 tttttcctgc cagatagcgc gttttaaaac gagtgaaatg ctcgcctaag cccgttgcgg
     6241 cttcggtatc gccggccata tctaacagtg cgatggctac ctcggcagta caatattggc
     6301 cttcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg acggaaatga
     6361 cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttca ggccaggtac
     6421 catcgagcat gataaatagc ggaggcttac cggcaggtgg tgtgaagatc acttcccgtt
     6481 gctcatcagc atacgaggcg ggaaagacca ccattggctg atagtccggg ttttgtacca
     6541 gatccagcaa atctagcgag ggttcggtac gcgaccattg aaacgcaacg gtatcaggca
     6601 aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg tcgaacatca
     6661 gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga cataatgtct
     6721 ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga aaaggacgtg
     6781 ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc ataagagagc
     6841 gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc cccggtaaga
     6901 ttaaagagat tcgttcagcc agctatcaaa cggcgctttc ggtactgcgc cattaagcat
     6961 gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa ttccaaagcg
     7021 actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt taccgctacg
     7081 ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc accacggtgc
     7141 ccagaagtcg atcaccacag gtagatcatc tttcagcaat ttgtcgagcg tttcaccggt
     7201 cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt ttgccgcatc
     7261 ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa cggtattcat
     7321 aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg tttctaatat
     7381 gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa taggtatgat
     7441 agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta atctgcgcgc
     7501 ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa agcggcaagg
     7561 tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac aacccgcgta
     7621 ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt cgccccgccc
     7681 gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agagtcgccg tggcgcacag
     7741 tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc atcagtggta
     7801 aaagttttat tgatccggaa gtgttgcgtt gtcagcgtgc ggaagaaacc cgcgtctacg
     7861 gcgaaaatgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt cgcgcctggt
     7921 ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca gcaaaccgca
     7981 aagcgtacca tgtggtagat gaagcggaac tgacaaaagc gtcaggcacg gaacatcacg
     8041 gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgctgcag tgggtaagcc
     8101 aggcaggcgc gcaggattgt gtactggcgc tggaaaacga atctaacccg cataacctgg
     8161 gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg cgttgtggtg caggatgcgg
     8221 cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag cacgttcagc
     8281 cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca ggttacaccg
     8341 tagtgaccac ttccagcgag caggggaaac cgctgttcaa aaccagtctg ccagcgaaaa
     8401 tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc gatccgaacg
     8461 atttgcgcgt gaagattgat ggtactggca acgttgccgg gctaaatatc tctgttgcaa
     8521 ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc agccgggtgg
     8581 caacgctgcc atccggcgtt ttcctgcaaa tttactcact ttctgccggt aatactggca
     8641 tccagtcaat cggtttctcg ccacgttgtt ccagccactg atttgccagc acaaaatggt
     8701 tgcagccaaa gaacccacga tgcgcagaaa gcggtgatgg atgcggcgct ttcagtatgt
     8761 gatggcgttg cttatcaata atcgcccctt tcttttgcgc atgtgagccc cacaacaaaa
     8821 acaccacgtc ttcgcggtgc tggttaatca ggctgattac tttatcggtg aaggtctccc
     8881 agccgaggct ggcgtgggaa tgtgcctgac ctgcgcgaac cgtcaacaca gtattgagta
     8941 gcagaacgcc ctgacgcgcc cagctttcaa gataaccatg attagggcgg gtgaaaccgg
     9001 gaatagtatt ttccagctct ttatacatat tcagcagtga cggcggtgtc gcaatgccgg
     9061 ggcgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga tcctggccga
     9121 gaatcaccac tttaacgtca cccaactctg taaagcggaa cgcgttaaag acatcttttt
     9181 gtggcggata gatagtgacg ccggactgcc gctcgctggc gacggtctgt agggtattaa
     9241 gaaaataggg ttgctgcttc tcttcagcca gcacatcatg ccaggttaat tcgtttgcca
     9301 tctcgctctc ccgcgaatct tcaatccgcc tagcttaact gcttctttct acagaacaaa
     9361 atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt aaaatttcac
     9421 acctgcataa gttgatgtaa aacaataaaa tccatttgca accatacttg atgtgtggtt
     9481 tttattgatt taaatcaaag attcaagggt gtttgaggag tatatataca ctcaagcaac
     9541 aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg ccggggaggc
     9601 atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg ctgaactctt
     9661 tctggctgct ggacagcgaa aaaggcgaag cgcgttgcat cgttgcgaaa gcaggttttg
     9721 cagaagacga agtggttgca gtaagcaaac tgggtgacat tgagtaccgt gaagttccag
     9781 tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca tctgaacgta aacgttctgc
     9841 gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag ctgaccatcc
     9901 gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag cgcgacgtta
     9961 ttgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta agcggaagag
    10021 ggcgacttct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat agaccagcag
    10081 cagcgcgagc acgatattta actggcgacc atactggcga aacaatcgct gaaacaggtg
    10141 ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca aaacgctgac
    10201 gccaattatc cagcttagcg cctgcgtttg cggcaggaca aacgtcgaca gtgctgtaat
    10261 gccatacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc aaaaactgat
    10321 tggttttgtc tgaagcccat cttcctttgt tggactggta gcgattttcc acgccagcca
    10381 gacaatatat gccgcccccg cccaactcaa cagatgcact gctgccgggt cgataacagc
    10441 cagtgaaaac gaaattcccg cgcatagcaa catgacgatt aaaaatccca gactcatccc
    10501 tgccagcacg cgagtactct gacgaaatcc gtgcgttgtg gctgagctaa gggcgagaat
    10561 attgttcggt cctggcgtca tggcggtaat cagggtgtaa gtccaaaaag cacttaaaag
    10621 ggtcggtgtc acttgtt
//
LOCUS       DAZKJR010000167        10492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKJR010000167 DAZKJR010000000
VERSION     DAZKJR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.168"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..1437
                     /gene="dcuC"
                     /locus_tag="ACHOWS_004438"
     CDS             70..1437
                     /gene="dcuC"
                     /locus_tag="ACHOWS_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFQ0049923.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCIIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTLFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAIGAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1481..1978)
                     /gene="sspB"
                     /locus_tag="ACHOWS_004439"
     CDS             complement(1481..1978)
                     /gene="sspB"
                     /locus_tag="ACHOWS_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFQ0049924.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1984..2622)
                     /gene="sspA"
                     /locus_tag="ACHOWS_004440"
     CDS             complement(1984..2622)
                     /gene="sspA"
                     /locus_tag="ACHOWS_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFQ0049925.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3017..3409)
                     /gene="rpsI"
                     /locus_tag="ACHOWS_004441"
     CDS             complement(3017..3409)
                     /gene="rpsI"
                     /locus_tag="ACHOWS_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0049926.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3433..3861)
                     /gene="rplM"
                     /locus_tag="ACHOWS_004442"
     CDS             complement(3433..3861)
                     /gene="rplM"
                     /locus_tag="ACHOWS_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0049927.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4080..5207)
                     /gene="zapE"
                     /locus_tag="ACHOWS_004443"
     CDS             complement(4080..5207)
                     /gene="zapE"
                     /locus_tag="ACHOWS_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFQ0049928.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5401..5799
                     /gene="zapG"
                     /locus_tag="ACHOWS_004444"
     CDS             5401..5799
                     /gene="zapG"
                     /locus_tag="ACHOWS_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFQ0049929.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5953..7320
                     /gene="degQ"
                     /locus_tag="ACHOWS_004445"
     CDS             5953..7320
                     /gene="degQ"
                     /locus_tag="ACHOWS_004445"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFQ0049930.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7410..8477
                     /gene="degS"
                     /locus_tag="ACHOWS_004446"
     CDS             7410..8477
                     /gene="degS"
                     /locus_tag="ACHOWS_004446"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFQ0049931.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8540..9478)
                     /gene="mdh"
                     /locus_tag="ACHOWS_004447"
     CDS             complement(8540..9478)
                     /gene="mdh"
                     /locus_tag="ACHOWS_004447"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFQ0049932.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9913..10383
                     /gene="argR"
                     /locus_tag="ACHOWS_004448"
     CDS             9913..10383
                     /gene="argR"
                     /locus_tag="ACHOWS_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFQ0049933.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2558 a         2670 c         2632 g         2632 t
ORIGIN      
        1 atataacatc attgttttca atctgccgtt tatgggattg accattttct tttgacacgg
       61 agttcaacaa tgttcggcat aattatatct gtcatcgtat taattacgat gggctatttg
      121 atcctgaaaa actacaaacc tcaggtggtg ctggctgccg caggtatctt cctgatgatg
      181 tgcggtgtct ggttagggtt cggtggtgtg ctcgatcccg ccaaaagcag cggctacttg
      241 atcgtcgata tttataatga aatcctgcgc atgctgtcca accgcattgc tggattgggg
      301 ctgtcgatta tggcagtggg cggttatgcc cgctatatgg agcgcatagg ggcaagtcgc
      361 gcgatggtga gcttgttaag ccgcccgtta aaactcattc gctcgccgta tattattctg
      421 tcggcaactt acgtcatcgg ccaaatcatg gcgcagttta ttaccagcgc ctccggtctg
      481 ggtatgttgc tgatggtcac cttatttccg acgctggtga gtctgggagt aagtcgtctc
      541 tctgcggtgg cggttatcgc aaccaccatg tccattgagt gggggattct ggaaacgaac
      601 tccatttttg ctgcccaggt cgcaggaatg aaaattgcca cttacttctt ccactaccag
      661 cttccggtcg cctcttgcat cattatctcg gtggcgatct cccacttttt cgtgcaacgc
      721 gctttcgaca aaaaagataa aaatatcaat cacgaacagg cagagcaaaa agctctcgat
      781 aatgtcccgc cgctctatta cgccatttta ccggtgatgc cgttaatcct gatgctcggc
      841 tcgctgttcc ttgcccacgt cgggctgatg cagtcagaac tgcatctggt ggtggtgatg
      901 ttactgagtt tgacggtgac gctgtttgtt gagttcttcc gcaagcataa cttgcgcgaa
      961 acaatggacg atgtgcaggc gttttttgac ggcatgggta cgcagtttgc caacgtggta
     1021 acgctggtgg tcgcgggtga aatatttgcg aaaggcttaa cgacgattgg cactgtcgat
     1081 gcggttatca ggggggcgga gcattctggt ctgggcggta ttggcgtgat gattattatg
     1141 gcgctggtca ttgccattgg tgccattgtg atgggctcag gcaatgcgcc gtttatgtca
     1201 tttgccagtc ttattccgaa tatcgcagcc ggactacatg taccagcggt tgtaatgatt
     1261 atgccgatgc attttgccac gacgctagcg cgcgctgttt cgccgattac tgcggtggtg
     1321 gtcgttacgt caggaattgc aggcgtttcg ccttttgcgg tagtgaagcg gacagcgatc
     1381 cccatggcag tcggtttcgt ggtgaatatg attgccacta tcacgctatt ttattaagcg
     1441 attaaaaaga caaaacaggc cgccagggcc tgttttcatt ttacttcaca acgcgtaatg
     1501 ccggtcgacc accgcgtggt ggctgcggag gttcatcgtc aggatgagtg tcatcatcgt
     1561 gatctggctt gtcgccatca ataaccgaca taacggtttc gttgtctgcc gatgcctctt
     1621 catcattcat gatgctggca tcttcatcgt aggcagcttc aggttcaaac atcgtgcctg
     1681 cgccattttc acgggcgtag atagccagca cggcagccag cggcacagaa acctgacgcg
     1741 gaatgccacc aaagcgcgcg ttaaagcgca cctcatcatt cgccagttcc agattgccga
     1801 cagctcgcgg cgcaatgttg agtacgattt gcccgtcacg cgcatattcc ataggaacct
     1861 gcacgccagg gagcgtcaca tccaccacca ggtgcggcgt gagctggtta tccagcaacc
     1921 actcatagaa tgcacgcagc agatagggac gacgtggtgt tagctgtgac aaatccatac
     1981 agattaactc cggcccagac gcatttcacg ttcagcttca gttaaagaag caaggaaaga
     2041 gtcacgctca aagacgcggg tcatatagcc tttcagctct ttcgcacccg ggccgctgaa
     2101 ctcgatgccc agttgcggca gacgccacag cagaggagca aggtagcaat cgaccaggct
     2161 gaactcatcg ctcaggaagt acggcttctg accgaagacc ggcgcaatcg ccagcagttc
     2221 ttcgcgcagt tgcttacgtg cggcatcggc ttcagaagct gaaccgttga tgatggtgtt
     2281 catcagcgtg taccagtctt tttcgatgcg atgcatgtac agacggcttt caccgcgagc
     2341 taccgggtaa acaggcatca gtggcggatg cgggaaacgc tcatccagat attccataat
     2401 gatgcgagat tcccacaggg tcagctcacg atccaccagg gtcggaacgc tctgattcgg
     2461 gttgaggtca atcagatcct gaggcggatt gtccttttcc acgtgttcga tctcgaaact
     2521 tacacctttc tcagccagca caatgcggac ctgatggcta tagatgtcag taggaccgga
     2581 aaacagcgtc attaccgaac gtttgttggc agcgacagcc atgaaaacct ccaggtatag
     2641 tcagaatttt tactgctacc agccaccagg tggccagtca gaagttgtgt tacccagtaa
     2701 ggaacgactc tctttgttcg aaaatcaaac aaaaaatgag caatacccga catttgggca
     2761 gaaaattgga tgatagttta ccagattttg cgacctttgt ggtgagtcga tgccggaaat
     2821 ggggaaaaag agatgcgctt tagtctgaaa tagttgactt agtcccttat tggcgatgtg
     2881 gtttttgttt tacctgtctg tcaggtggca gcaaaaagca acttcccatt ttttacgctg
     2941 atgcagattt tagccataaa aaaacccgcc gaagcgggtt ttttcgcaaa ttgttttctg
     3001 ccggagcaga agccaattaa cgtttggaga actgcggacg acgacgtgct ttacgcagac
     3061 cgactttctt acgttcaacc tgacgagcgt cacgagtaac gaagccagct ttacgcagtt
     3121 cagaacgcag ggactcgtcg tattccatca gagcgcgggt gataccgtga cggatcgcac
     3181 cagcctgacc agagatacca ccacctttaa cggtgatgta caggtccagt ttctcaacca
     3241 tgtcgaccag ttccagcggc tgacgaacta ccatgcgagc agtttcacga ccaaagtact
     3301 gttccagaga acgttggttg attacgattt taccgttgcc cggtttgatg aaaacgcgag
     3361 ctgcggaact tttgcggcga ccagtgccgt agtattgatt ttcagccatt gcctataatc
     3421 ccgattagat gattagatgt caagaacttg cggttgctgt gccgcgtggt tgtgctcgtt
     3481 acccgcgtaa actttcagtt tacggaacat agcacgaccc agcgggcctt ttggcaacat
     3541 gcctttaacc gcgatttcaa tcacacgctc aggacggcga gcaatcatct cttcaaaggt
     3601 cgcttgtttg ataccaccga tgtggccggt gtggtgatag tacactttgt cagtacgctt
     3661 gttgccggtt acagcaactt tgtcagcgtt cagaacgatg atgtaatcac cggtatctac
     3721 gtgcggagtg tattccgctt tgtgcttacc gcgcaggcga cgagccagtt cagtagccag
     3781 acggcccaga gttttaccgg tcgcgtcaac aacataccag tcgcgtttta cggtttctgg
     3841 tttagctgta aaagttttca ttaaaagctt acccaataaa aagttacacg ttggtgaaca
     3901 cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct accccttcga
     3961 atagcctatg ccagcacaca aaaagttttg ggaaaaaaac tttcttgtaa cgtggggtcg
     4021 caggattata gagaagtcgg ggtcaaagat cgaccccttt ttgtgatttg tggcaggttt
     4081 taacccgcca aatgctcgcg cttcagatac tcttcgcttt gcatctcttg cagacgtgac
     4141 aggcaacgct ggaactcaaa tttcagccgc tcgccctgat aaatgtcata tagcggcact
     4201 tctgcactca ccactaattt gacatggcgc tcgtaaaact catccaccag cgcaataaaa
     4261 cgtcgcgctt cgctctccat caaccgcgtc ataactggta catcaaacaa catgactgtg
     4321 tgaaagaggc gtgagagcgc aatatagtca tgctgactgc gggcgtcgac gcacagcgta
     4381 gtaaaagaga ccgccagcgt ctggttctcg acgcccattg tcgccagcgg ccgatggttg
     4441 atttctaatg tcggtgaatt ttctcgtttc gcccccgcca gcgccaacca tagtttatcc
     4501 atttgcgccc gggtttcatc gttgaggggc gaaagccaca gatgcgcctg agtgagtgta
     4561 cgcagtcgat aatcaacacc agcgtccacg ttcattacat cacaatgctg tttaatggca
     4621 tcgattgcag gcaaaaaacg cgcacgttgt aggccatttc gataaagttc gtccggcgga
     4681 atatttgacg tcgctaccag ggtaataccg cgggcgaaca gggctttcat cagaccgcca
     4741 agtagcatgg catcggtaat atcagaaaca aaaaattcgt caaaacagag cacgtcagtt
     4801 tctgctttaa agcgatcggc aataatttcc agcggatcgg tctgcccctg caaagcagtt
     4861 agctcttcat gtacccgcag cataaaacgg tgaaagtgca gacgctgttt ccgctctccc
     4921 ggcaggcttt gatagaaaag gtccatcagc caggttttcc cgcgtcctac accgccccac
     4981 atatataagc cacgcactgg cgtatgcttt gtgtcttcgc gtttacccca cagcttaccg
     5041 acccgcgcca ttagcccact cgtcctgggg gctggaggcg tgctattgat gagttcctga
     5101 taaataattt ccaggcggct gacggcctct ttttgaacgt cgtcgggttg atggctgcct
     5161 tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc tttgcatgat cttattgtta
     5221 ttccttgaat aatcggtgcg ccgttgttca cggttgacga aaaaaaggcc gttctacact
     5281 acgcgatatg cagtcgggat tccacttctg tggaattaac ggttatagtg gcataatcag
     5341 ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca aaagacaacg ggagatgttc
     5401 atgacctggg aatatgcgct aattgggtta gtcgtcggca tcattattgg tgctgtggcc
     5461 atgcgttttg gtaatcgtaa actacgccag caacaggcgt tgcagtacga actggaaaag
     5521 aataaagctg aactggacga gtatcgcgaa gagctggtta gccactttgc ccgcagcgcg
     5581 gaattactgg ataccatggc gcacgactat cgccagctgt atcaacacat ggcgaaaagc
     5641 tccagcagcc tgctgccgga actgtctgct gaagcaaacc cgttccgtaa tcgtctggca
     5701 gagtctgaag ccagcaacga tcaggcaccg gtgcaaatgc ctcgcgacta ttctgaaggc
     5761 gcatccggcc tactgcgtac tggcgcgaag cgcgactaat ttatttttcg ggcgcagcca
     5821 ttgcgccctc ctcttctctc cctccccgac tatcatttaa tctggtgtct cattgttagc
     5881 cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct tttcttatca ttcaggtacg
     5941 agagtaggaa taatgaaaaa acaaacccag ctgttgagtg cattagcgtt aagtgtcggg
     6001 ttaactctct cggcgtcatt tcaggccgtt gcgtcgattc caggccaggt tgccgatcag
     6061 gcccctctcc ccagtctggc cccaatgctg gaaaaagtgc ttccggcagt ggtgagcgta
     6121 cgggtggaag gaacggccag tcagggacag aaaatcccgg aagaattcaa aaagtttttt
     6181 ggtgatgatt taccggatca accggcacaa cccttcgaag gtttaggctc cggtgtcatc
     6241 atcaacgcca gtaaaggcta tgtgctgact aacaaccatg tgattaatca ggcacagaaa
     6301 atcagtattc agctcaatga tgggcgcgag tttgatgcaa aactgattgg tagcgatgac
     6361 cagagcgata tcgccctgtt gcaaattcaa aacccgagca aattaacgca aatcgctatt
     6421 gccgactccg ataaattgcg cgtcggtgat tttgccgtgg cggtcggtaa cccatttggc
     6481 cttgggcaaa ccgccacctc tggcattgtt tccgcattag gccgcagtgg gttgaatctt
     6541 gaaggtctgg aaaactttat ccagacagat gcttccatta accgcggtaa ctccggcggt
     6601 gcactgttaa atcttaacgg tgagttaatt ggcatcaaca cggcaatcct tgcgcctggt
     6661 ggcgggagcg tcgggattgg atttgccatc cccagtaata tggcgcgaac actggcgcag
     6721 cagcttatcg actttggtga aatcaaacgc ggtctgttag gcatcaaagg cacagagatg
     6781 agtgccgata tcgccaaagc cttcaacctt gacgtgcagc gtggcgcgtt tgtcagcgaa
     6841 gtgttgccag gttctggctc ggcaaaagcg ggcgtcaaag cgggcgatat tattaccagc
     6901 ctcaacggca aaccgctgaa tagctttgct gagttgcgct ctcgtatcgc aaccaccgag
     6961 ccgggcacga aagtgaagct tggcctgctg cgtaacggca aaccactgga agtagaagtg
     7021 acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa tgatcacgcc agcgcttgaa
     7081 ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca aaggtattaa gatcgatgag
     7141 gttgtcaaag gaagcccagc tgctcaggca ggcttgcaaa aagacgatgt gatcattggc
     7201 gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta aagtgctggc ggcaaaaccg
     7261 gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca tctatctgct gatgcgttaa
     7321 tgtcgtaaac cgggcatcag gctgacgtgt gatgtccggt taactcgtgg tatgctgctg
     7381 ccgttccctt ttttaatgac gcctccatca tgtttgtgaa gctcttacgt tccgttgcga
     7441 ttggattaat tgtcggcgct attctgctgg ttgccatgcc ttcgctgcgc agccttaacc
     7501 cgctttccac tccgcaattt gacagtaccg atgagacgcc tgccagctat aatctggcgg
     7561 ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg tggtttgaac accaactctc
     7621 acaaccagct tgagatccgc accctgggat ccggtgtaat catggatcaa cgcggttata
     7681 tcatcaccaa taaacacgtc atcaacgacg ccgatcagat catcgtcgcc ttacaggatg
     7741 gacgtgtttt tgaagcgtta ctggttggct ctgactctct aaccgatctg gcggtgctga
     7801 aaattaacgc cacaggcggt ttaccaacga ttccgatcaa tccacgccgc gtaccgcaca
     7861 ttggcgatgt ggttctggcg atcggtaacc cgtacaacct cgggcagacc attacccagg
     7921 ggattatcag tgccacgggt cgaatcggtc tgaacccgac cgggcggcaa aacttcctgc
     7981 aaaccgatgc ttccattaac cacggtaact ctggcggcgc gctggtgaac tcgctgggcg
     8041 aactgatggg cattaacacg ctgtcgtttg ataagagtaa cgatggcgaa acgccggaag
     8101 gtatcggctt tgcgattcct ttccagttag cgaccaaaat tatggataaa ctgatccgtg
     8161 atggccgtgt gatccgtggc tacattggca ttggtggacg tgagatcgcc ccattacatg
     8221 cgcagagtgg cggcatggat caactgcaag ggatcgtggt taacgaagtg tcgcctgatg
     8281 gcccagcggc aaatgcaggc atccaggtca acgatctgat tatctctgtg aataacaaac
     8341 ccgctatttc cgccctggag acaatggatc aggtcgcgga aattcgccct ggttcggtga
     8401 tcccggttgt cgtgatgcgt gacgataaac agttaacatt gcaggtcacc attcaggaat
     8461 atccggcgac caactaaggt cagcgtaaaa aaacaaaaac cggagtctgt gctccggttt
     8521 tttattatcc gctaatcaat tacttattaa cgaactcttc gcccagggcg atatctttct
     8581 tcagcgtatc cagcatacct tccagcgcgt tctgttcaaa tgcgctcagg gtaccgatag
     8641 atttacgctc ttccacgccg tttttaccca gcagcagcgg ttgagagaag aaacgtgcgt
     8701 actgaccgtc gccttcaacg taggcacatt cgacaacgcc ttgttcgccc tgcagtgcgc
     8761 gaaccagaga cagaccaaaa cgtgcagctg cctggcccat agacagggtt gcagacccgc
     8821 caccggcttt cgcttcaacc acctcagtac ccgcgttctg gatacgtttg gtcagatcag
     8881 ccacttcctg ctcggtaaaa ctaacgccag gaacctgtga cagcagcggc agaatggtaa
     8941 caccagagtg accgccaata accggcactt caacttcgcc tggctgtttg cctttcagtt
     9001 ccgcaacaaa ggtgttggaa cgaatgatat ccagcgtggt aacgccgaac agtttgtttt
     9061 tgtcataaac accggctttt ttcagcactt cagcagcaat cgcaactgtg gtgttaaccg
     9121 ggttagtgat aataccaatg cacgctttcg ggcaggtttt cgcaacttgc tgtaccaggt
     9181 ttttcacgat gccggcgtta acgttaaaca ggtcggaacg atccataccc ggtttacgcg
     9241 ctacacctgc agagataaga acgacatctg cgccttccag cgccggagtc gcatcttcac
     9301 cagaaaaacc tttgattttc acagcagtag ggatatggct cagatcgaca gccacaccgg
     9361 gagtcactgg agcgatatca tacagagaga gttctgaacc tgaaggcagt tgggttttta
     9421 acagtagtgc aagcgcctgg ccaataccgc cagcagcgcc gaggactgcg actttcatcc
     9481 taaactcctt attatattga taaactaaga tatgttgctc cgctgccgcg accttaatcc
     9541 acaaaattgc cgtttacaat gaccacatct caagaatgtg tagtcacgca agtttagcgt
     9601 ttatgcattt aattgccgta atcaggaacc taacttacgt aattaacagt cgcttatgca
     9661 ttagcgcaac attccaacag gtggtgacaa tataccctac cgttcagcca aaacaacatc
     9721 aatttgataa caattaattt acttttaagc agaatttgca tgccgtgacg caggcatgtt
     9781 tctcaataac gaaatttgat aaaatcccgc tctttcataa cattatttca gccttcttca
     9841 gggctgactg tttgcataaa aattcatctg tatgcacaat aatgttgtat caaccaccat
     9901 atcgggtgac ttatgcgaag ctcggctaag caagaagaac tagttaaagc atttaaagca
     9961 ttacttaaag aagagaaatt tagctcccag ggcgaaatcg tcgccgcatt gcaggagcaa
    10021 ggctttgaca atattaatca gtctaaagtc tcgcggatgt tgaccaagtt tggtgctgta
    10081 cgtacacgca atgccaaaat ggaaatggtt tactgcctgc cagctgaact gggtgtacca
    10141 accacctcca gtccattgaa gaatctggtg ctggatatcg actacaacga tgcagttgtc
    10201 gtgattcata ccagccctgg cgcggcgcag ttaattgctc gcctgctgga ctcactgggc
    10261 aaagcagaag gtattctggg caccatcgct ggcgatgaca ccatctttac tacccctgct
    10321 aacggtttca ccgtcaaaga cctgtacgaa gcgattttag agctgttcga ccaggagctt
    10381 taatctctgc cccgtcgctt ctggcggcgg ggaaaatgtt gcttttccct ctcacccccc
    10441 ctgctttctc ctgcgattaa tttaacgaat agtgcgtttt atcgcgacat at
//
LOCUS       DAZKJR010000168        10406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKJR010000168 DAZKJR010000000
VERSION     DAZKJR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.169"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            73..2160
                     /gene="iha"
                     /locus_tag="ACHOWS_004449"
     CDS             73..2160
                     /gene="iha"
                     /locus_tag="ACHOWS_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFQ0049934.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSSLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            complement(2378..2528)
                     /locus_tag="ACHOWS_004450"
                     /pseudo
     CDS             complement(2378..2528)
                     /locus_tag="ACHOWS_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3022..3264
                     /locus_tag="ACHOWS_004451"
     CDS             3022..3264
                     /locus_tag="ACHOWS_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049935.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            3555..3923
                     /locus_tag="ACHOWS_004452"
     CDS             3555..3923
                     /locus_tag="ACHOWS_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049936.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            3927..4139
                     /locus_tag="ACHOWS_004453"
     CDS             3927..4139
                     /locus_tag="ACHOWS_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049937.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQTFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEAESS"
     gene            4136..4408
                     /locus_tag="ACHOWS_004454"
     CDS             4136..4408
                     /locus_tag="ACHOWS_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFQ0049938.1"
                     /translation="MTDITDRHTLRRMSWSELTTAAQEAELNRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNPRGRSVCLRPQHPRR"
     gene            complement(4717..5548)
                     /locus_tag="ACHOWS_004455"
                     /pseudo
     CDS             complement(4717..5548)
                     /locus_tag="ACHOWS_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5606..5794
                     /locus_tag="ACHOWS_004456"
                     /pseudo
     CDS             5606..5794
                     /locus_tag="ACHOWS_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5863..7089)
                     /locus_tag="ACHOWS_004457"
     CDS             complement(5863..7089)
                     /locus_tag="ACHOWS_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0049939.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(7162..8184)
                     /gene="iolG2"
                     /locus_tag="ACHOWS_004458"
     CDS             complement(7162..8184)
                     /gene="iolG2"
                     /locus_tag="ACHOWS_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HFQ0049940.1"
                     /translation="MQKLRCGIIGLGRVGKMHVENMYLLPQLDIVCAADSFIEEMSDW
                     LSSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAGKHIFCEKPLSMRE
                     DEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQPQMIKITSRDPEL
                     LPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDPTLKNIDDVDTLVV
                     MLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAVKVYNSQNCIMKNP
                     LPDFALRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIAAKQSLKSGLPVKI
                     KTDIQL"
     gene            complement(8357..10297)
                     /gene="iolD"
                     /locus_tag="ACHOWS_004459"
     CDS             complement(8357..10297)
                     /gene="iolD"
                     /locus_tag="ACHOWS_004459"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFQ0049941.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
BASE COUNT         2883 a         2641 c         2328 g         2554 t
ORIGIN      
        1 atataaatta ttctcattat tgtttgtatt tgtgtattgt cttgccgatt aacatgatcg
       61 gagattagta atatgcgaat aaccactctg gcttccgttg tcattccctg tctcgggttt
      121 tcagccagca gcatggcagc agaggatgtg atgattgtct cggcatccgg ctatgagaaa
      181 aaactgacca acgcagccgc cagtgtttcc gtaatcagtc aggaggaatt gcagtccagc
      241 cagtaccacg atctggcgga ggcactgaga gctgtggagg gtgtggatgt tgaaagtggt
      301 acgggtaaaa ccggtgggct ggaaatcagc atccgtggaa tgcccgccag ttacacgctg
      361 atactgattg atggtgtccg tcagggcggg agcggggacg tgacgcctaa cggtttttct
      421 gccatgaata cagggttcat gcctcctctg gccgcgattg agcgtattga ggttatcagg
      481 gggccgatgt ccacactgta tggctctgat gcgatgggtg gtgtggtgaa tatcattacc
      541 agaaagaatg cagacaaatg gctctcttcc gtcaacgccg ggctgaatct gcaggagagt
      601 aacaaatggg gcaacagcag ccagtttaat ttctggagca gtggccctct tgtggatgat
      661 tctgtcagtc tgcagatccg cggcagcaca cagcagcgtc agggctcatc ggtcacatca
      721 ctgagcgaca catcagccac gcgtattcct tatcccacgg agtcacagaa ttataatctt
      781 ggtgcgcgta ttgactggaa ggctacggag caggatgtgc tctggtttga tatggatacc
      841 acccgacagc gttacgataa ccaggatggt caactgggta gcctgacagg gggatatgac
      901 catactctgc gctatgagcg aaacaaaatt tcggctggct atgatcatac tttcaccttc
      961 ggtacctgga aatcgtatct gaactggaac gagacggaaa ataaaggtcg tgagcttgta
     1021 cgtagtgttc tgaagagcga taaatggggg cttgccggta aaccgcggga gcttaaggaa
     1081 tctaacctta tcctgaattc attgctgctt acccctctgg gagaatctca tctgcttact
     1141 gtggggggag agtatcagag ttcgtccatg aaagatgggg ttgtccttgc cagcacaggt
     1201 gaaactttcc gacagaaaag ctggtcagta tttgctgagg atgagtggca tctcacggat
     1261 gtacttgcgc tgactgcagg cagtcgctat gaacatcatg atcaattcgg gggacacttc
     1321 agtccgcgtg catatctggt ctgggacgtg accgatgcct ggacgctgaa aggcggtgtg
     1381 accacgggat ataaagcccc caccatggga cagttacata aggggatcag cggtgtatcc
     1441 gggcagggac gtactaatct actcggtaac cccgaactga agccggaaga gagtgtaagt
     1501 tatgaggcgg gggtatatta cgataacctc tccggtctga atgccaatat aacaggcttt
     1561 ataacagact tctccaacaa gattgtctct tattccattg atgataacac caatagctat
     1621 acaaacagcg gaaaggcccg cttgcatggt gtggaattcg ccggcacatt gccgctgtgg
     1681 tcagaggatg tcactctgtc gttgaattac acctggaccc aaagtgaaca acgtgatggt
     1741 gataacaaag gtgcaccgct gagttatacc cctgaacaca tggtgaatgc gaaactgaac
     1801 tggcagatca ccgaagaggt ggcatcatgg ctgggtgccc gttatcgcgg gaaaacacca
     1861 cgtttcaccc ggaattattc gtcactgagc gctgtacaga agaaagtgta tgatgagaaa
     1921 ggagaatacc tgaaagcctg gacggtggtg gatgcaggtc tgtcgtggaa gatgacggat
     1981 gccctgacgc tgaatgctgc ggtgaataac ctgctcaaca aggattacag tgacgtgagc
     2041 ctgtacagtg ccggtaagag ttcgctgtat gccggtgatt acttccagac gggatcatca
     2101 acaacaggat atgtgatacc tgagcgaaat tactggatgt cgctgaacta tcagttctga
     2161 taataacaaa acgctatcac tgacggtaga atacgttgcc actgcaactc ctggcggaac
     2221 agtggcaacg tattaggtta agtgcatttc cgatccgcta atgagatttc gttaccaaca
     2281 actaatatcg tcacaggaaa tgcacggatt atttttaact tatcatttac atacttgtcc
     2341 agagtgtaag cgcaccgtga aggacgtggg gtaaaaatta gtttacagag agagtgacgt
     2401 tccagggcaa caactctttc atgcggttgg caggccaggt gttgattaca ctgatcacgt
     2461 ggcgtagcca cgtttccggc tcgattccgt taagttttgc agctaccgat caggctgtac
     2521 atcactgccg cactatcgct cgtcatctca aagtccggtc tcgtcagcag gaaggtatca
     2581 ttctctcccg ccatttttcc gggggcccgg tcagataagt ccctttgtct atcgctgact
     2641 cctggctcat aacccggtta gcagaatgca gggtcaccac ctcgccacga ccgaatccgg
     2701 ataagtcaat tgcaccttct caatcgccat tttgtcacaa ttaagtttga taaagcaggt
     2761 cacctctgca ctgaatctgc tccagtgcct cattggtctc cgtccccccc tttaaactgc
     2821 cggataagac tgctgcacag cgccttcccc ggaatcaaat cactttccct gtccgccaca
     2881 atccgcttca tgctacctcg tattttgttt cagagaccgg aatctgccag aaagtgacta
     2941 cgcgtgcaca tatcggtttt ctgggttaca tgcatcgtgt tattttctga aatgcaaaat
     3001 aataaaaaac aaggacacat aatgaaggat gaaatacaca gtaccagcat caacccggat
     3061 gttgttgagc agtttctggc ccttatgaac acacattgta aatcctcaga agaggcgctc
     3121 gccatgttga gtacgctttg ttacaacctg atggtgacat gtggcatcca ttccatggac
     3181 agccagatgc tcaatgggca tgggatatcc atgcaacttc aggggattga tattgccgaa
     3241 aggccaggta agaatattca ctaacctgaa cagggcctga aaacgctgtc ttttcaggcc
     3301 tctgcaggca aaaactgatg acaacaaaat ttctgcgttt gtaccccacc gatgaaaaag
     3361 acaaaaactc acaccgggct tctagccagt aaagataaaa cccgacgtgt cagcctctac
     3421 gaaacgccaa cggcatggtg tatcaggggg caggagtgct acagcaaaag tacaggacgc
     3481 cgctgtggca gccacgacag tctgagcaga ctgcgcctag acagcattaa gcctgttgaa
     3541 tgagtggtgg caccatgaaa gataacaaca caaccgccac cccagaagcc tggaaaatgc
     3601 gttttctggc caacgggaca tccggcacat ggttttacac tgacgacatt gcagtcactg
     3661 acgcgtggct tcgcgcgcac ccggatgatg tggaggttat ccctctctgc cggataccac
     3721 cccgtcagca ttcccatgaa caacatattg aagaactccg cgcagccctg gcccggatgc
     3781 tgcatatgca tcatatgatg ctggaaacca cgcattacag ggaaaacatc accgatgccc
     3841 gttgtatccg tgaaatgcat gaagctccat tacaggcgct caatattctg gccagatacc
     3901 cgcataaaaa ggacgaggtg tgacgcatgt ggctactgat tttctggctt tcatctccct
     3961 ttaatgtctc ttccggtgat gcccggcagc ccacactggc ggtgcacagc cagacatttc
     4021 ataccgaacg ggcctgtcgc gatgcattgc gcgccattaa aaaggagaat gttggcgaat
     4081 tttctctgcg gggcgtttgt gtttctgacg gaaacacgaa ggaggcagag agctcatgac
     4141 ggatattact gacagacata cacttcgccg catgtcctgg tcggaactta ccacggcagc
     4201 gcaggaagca gaattaaacc gtgattacga gcgtgccaga atagtgtggt cattcgcctt
     4261 acatgttgca acaaccacga ttaacaaaaa tctggccata gctcatattc gacgctgcga
     4321 tactttgttg cataaatcaa aaacagtacc cggaaacaac ccgagaggcc ggtcagtgtg
     4381 cctgagacct caacatcccc ggagatgact gacattccgg actgacgcag ttgctgcata
     4441 tctgtggcaa tacgttgtat ccagccagac aggctatccc gcgtagtgac aattaacccg
     4501 ctttcctgca ccagcttcag ccgggccgtc ggggatttta ccagccggaa atatcagtaa
     4561 aacaacatat cattatgcca tggtgtacgc ggccagtgta tgcttactta aggaatgagg
     4621 gataaattgg atattattac ttcactttgc ttttttatga gattcatcac ataagtgggg
     4681 ttttactgtt aaataatctg aatttatttg atatccgtaa agcagatgtc tcagttcact
     4741 gtttccaact tttgttattc ctctggggcg aatgctgttt ccgctggaat attcacctgg
     4801 caccagtccc agaaaagcca tgagctgttc aggattggaa aatcgcgaaa aatcacccag
     4861 ttcagcaacc agggttattg cgattagctg accaacacct ttaagagcct gcaactggca
     4921 gaccagattg cacagagatc actcaggtaa caggcgagat atttcctgat cgagttgaag
     4981 tatgcgatct tcaatctgct ccagattctg acaataatgt tgaaagcaat ctgttgagat
     5041 agcagaccaa atgattgatt aactaaccag gttcgatgtt tgccggttca gtgcttcctg
     5101 gcataacgtt tgtctgttcg tgtcacgttt tgcagcggca cgggctctga ttaaatcccg
     5161 catagcttca tgagtcagct cagggagcca gactggggga acctcaccat ccctcagtaa
     5221 cctggcgaga gaaattacgt cccgatggtc atttttgatc ctgtctgccg gtgatttggg
     5281 tatacgggaa gggggcaata accaggcatt ttatattcat cactgtgagt tgatgatata
     5341 gcccaaaccc acagggacca ccttcatagc aggaaagaat gtttccgtaa ggtccctgca
     5401 atttctgaca taatcgatgt acagccaagg cttcgttgtt aatcgtacca taataacgaa
     5461 cttcgccaag ccattcaggt gtagcaatcg ccaaggtttc tttatgaaca tccagcccga
     5521 cgtaagctgc aatattttcc gaatccatga tgacctctgg agtatcccac caatgctata
     5581 gtgagtatat aaaggtcata tcatctacgt gttcttcggc aacgatcaca gtggagagcg
     5641 tggagcgcta ctgtacgggc tgattggcac ctgccgactg aacagtatcg atccggaagc
     5701 gtatctgcgc catattctga gcgtactgcc ggaatggcct tccaaccgtg ttgacgaact
     5761 gctgccatgg aacgtagttc tcaccgataa ataagcgtca atacggcatt cgattaatgc
     5821 acttacagct aaatggagtt tttgaacaat atttgacgtt atttatttct caccaggaac
     5881 tttaagaaac tgaaccagga tgccactaaa taaatagagt cctgccatag tatacataac
     5941 cacgcctgta ccaaaacctg tcagaatgga taccaacgca ggtccgacaa aatttcccag
     6001 gccagaaccg aaattcaccg ctgagtttgc tgcccccttc ttatccggtg ccatcatcgg
     6061 taccagcggc gtcatcggca caaatcccgc cagccccgct ccccacaggc acattgccag
     6121 cataaaagca acgtaattat gcccccacac tatcggcacc cagtagacaa tcagcgtggc
     6181 aaacccggtc agaagactgc caaaaatctg gatagtacgg cgaaaaccta ttttatcccc
     6241 catccagccg aaaataatgt tgaaaatctg attagaaata aaggttatac cccagatact
     6301 cgcccactcc tcaagggtaa actgtttatc cagcaaaaac agcggcaaca ccactacaaa
     6361 accataggtg ccgagtccat tgattgtttt aacaatcact gccagaccaa ttcggggtcg
     6421 ttgatacata atggaaatac ctgccgacaa ctctttaaag actgacgtag ttcttggctt
     6481 cagaatgaac gcattgcgct caaagaccag catgaaaatc cccccaagca gtgcaacccc
     6541 aatcccggcc cacataacat taatatgccc aaggacagga attaacgaag cagctactac
     6601 cggaccaatg atggtaaaac ctaagttata tactacccaa aacagcgacg ttccaattcc
     6661 ctgttgatga ttatcgagtt gaatggtcag tcggacaaga aaggaatagc agaccaacgg
     6721 ataacctact ccccgcagca tatagctaat gagaattgta ctaaaatcat ggctgggaat
     6781 cgcaaaatta agaaacgcga tagaagcgat caggtaaacg gagaaaccag caagcataac
     6841 ctttctgggg ccaaacatat ccacacagac accggataac caggaagtta cggcaaccac
     6901 aattccgtag gcggcaaata tctgtccgat ctgagactga tcaaatccct gagtattcag
     6961 ccaggcagat agccaactat tttcaataca gccgcccaac ataaataaaa atgttcccag
     7021 gtagctccag ataaactgac ctggctttat ataaaatgtc tgaggcaaca ttgcctgcat
     7081 atctttcatt ttataaccct cagtacggat aatctctcgc tcgtaatagt gcgagagacc
     7141 cttcgctatt tacaaatgca actaaagttg aatatcagtt tttatcttca ctggcagtcc
     7201 actttttaat gattgttttg cagcaatggc aatacgctgc gccaataatg catcttcacc
     7261 agtacagaca atgggtgtat gattcagaac agaatcaata aagtgtaata tttccgttct
     7321 ataagcttca cgatagcgta gcgcaaaatc aggcaaagga tttttcatga tacagttctg
     7381 actgttatat accttcaccg ccgattcact gacattgtcg gcatacgcca tccctgctga
     7441 accaaagacc tcaacccgtt ggtcgtagcc ataaaccgcc cgacgactat tatcaattag
     7501 cgcataacca ccgtttctga aggtcagcat caccacgaga gtatctacgt catcaatatt
     7561 tttcagagta ggatcaatca atgtattgcc tttaacataa acctcgctaa cttcatcctg
     7621 cataataaag cgagccatat caaagtcatg catagtaaaa tcaaatatga gtccacctat
     7681 cctcttgatc aaatcgtgcg gcaacaactc tggatcacgt gaggtgatct ttatcatctg
     7741 aggctgacca atcttgcctc tgcgcaccag ttcatacacc tcccgaaact ggggatcaaa
     7801 acgacgatta aaagcaatct gcagtttgac tcctttctct ttaaccgccc ttagcaccgc
     7861 aagggaagct ttctcatctt ctctcatgct gagaggtttt tcgcagaaaa tatgtttacc
     7921 cgcctggact gctgccgtaa cgatctcttc gtgcatatct gtcgaggtga aaataaacac
     7981 cgcctcaata tcatcacgct gtaacatttc ctgataatct ttatatcctg aggttatatt
     8041 taccgaactc agccagtcgc tcatttcctc aataaaactg tcagcagcac agacaatatc
     8101 taattgagga agtaaataca tattctcaac atgcattttc cctacccggc caagaccaat
     8161 aatcccacat cgaagctttt gcatttatac ctcgtcatat catgatatag taaatataaa
     8221 acgacaaaag aaaatcccct ttatccaaca cggtacgaaa gactttattt tgtagtttca
     8281 acgaacaaat acttttaccg taaaaaacgt caggttgcag aaaggcgccc tcctgcaacc
     8341 ttaaaaactg aattaattag tactgtcgcg cttgtccaat atgctcatca agcatacgag
     8401 ctactgcctg gatgcgttcg ctacgcgaca cgcgagcgcc accaacacgc caccagctca
     8461 gatatttatg cgccattgtt ttaggcagta ctttaatgtc tatcaacgta ctcaccgtct
     8521 gtttacgtgc atcctccaga gcttcaagca gttgtccaat gctggtgacg cgatagcttt
     8581 tacagccata tccttcggca atccgggcaa aatccacagg aatgagtccc ccctcctgac
     8641 gaccgttatc ctgtttatgg aaacgaaact cggtaaagaa actgtccata ccgtgttcca
     8701 tttgaagatt atttatacaa ccattagtca tgttatccag caaaataaca ttaattttct
     8761 tccccatctg gaccgaagtt accagctcag aatgcagcat cataaatgag ccgtcgccaa
     8821 cgaagctata tacctcccgc tccggttgcg ccaacttcgc ccccaatgcg gcattgacct
     8881 catagcccat acaggaatag ccatattcta cgtggtaatc attatcgcca cggctttgcc
     8941 agatgcgctg cagatcgcct ggcagacttc ccgccgcagc aaccaccagc gcgttttctg
     9001 gcagcattcg atttagtact ccaagaacgc gcgtctgtgt cagccatgaa tgagtaattt
     9061 cattgaactc ttcaaacact ttttgagtat ccatatgatc cacaatttcc ggtttgaaat
     9121 ccttaccgct gtattccgct gcatagacgc gatccacttc cgccagatat tgagaacgaa
     9181 cacgatggat cgttttcccc cattgtgata tgtaaccttc cggagccagt cgttcgcaaa
     9241 gctgcgtcag ggcaacacgg gcatcagcct gcaattgcac tccatccagt ttgaagacgt
     9301 caaaacggct gacgtttata ttcagatagc gcacatcagg attctggaaa atccatttag
     9361 aagaggttgt gaaatcggta taacgcgtac ccacaccaat aatcagatcg gcctcctgag
     9421 ccagcaaatt cgccgccagc gatccggttt caccaatccc accaacattc agcggatgcg
     9481 cagaactgag cgcaccttta ccagcctgcg tttcagcaat cggcagatga tattgttcgg
     9541 caaatcgcag cagttcctgt tcagactgcg aatacttcac acccccaccg caaataataa
     9601 ttggtctctg gtggcgaaga ataagagcaa ctgcatcatt cagcattagc tctgttggca
     9661 cgtggcgctc aagacgatag acccgacgtg caaaaaaata gtctggataa tcccaggctt
     9721 ctccctgaac atcctgcggc aaacaaagcg tcacagctcc cgtatcagcc ggatcggtaa
     9781 gcacccgcat cgcgctaatg caggcgctca ttagctgttc cggacggata atgcgatccc
     9841 aatagcgact cactgcacga aaagcatcat tggtgctcag cgtcaggtca tgctcctgtt
     9901 caatctgctg caacacagga tcgggttgac gcgatgcata cacatctcct ggtagcagaa
     9961 gcaacgggat acggttcgcc gtcgcagtcg ccgccgccgt gatcatatta gcggcaccag
    10021 gccctacgga ggaggtgcag gcataaatct gctgacgacg catctgacgc gcaaatcctg
    10081 ttgctatatg tgccataccc tgttcattgc gtccttgatg aacgctaagt tgaccacact
    10141 cttcttccag agcctgccct aatcccacga cattcccatg gccaaaaatt gcgaaaatac
    10201 cttttacaaa cttgatttcg cgaccgtcca cttcaatgta ctgattatca agaaagcgca
    10261 ccaaagcctg cgccatagtc aacctgattg ttttcatgca ctccacctat cttatcaacc
    10321 gtttgactgt cgttacctac gtacgcactg ctacagcccc gggcataaaa cactcaccac
    10381 aagaaaaaaa ttccgcaaac gcaatt
//
LOCUS       DAZKJR010000169        10322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKJR010000169 DAZKJR010000000
VERSION     DAZKJR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.170"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..379
                     /locus_tag="ACHOWS_004460"
                     /pseudo
     CDS             174..379
                     /locus_tag="ACHOWS_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(468..1604)
                     /gene="yibH"
                     /locus_tag="ACHOWS_004461"
     CDS             complement(468..1604)
                     /gene="yibH"
                     /locus_tag="ACHOWS_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0049942.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1607..1969)
                     /gene="yibI"
                     /locus_tag="ACHOWS_004462"
     CDS             complement(1607..1969)
                     /gene="yibI"
                     /locus_tag="ACHOWS_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFQ0049943.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2506..4419
                     /gene="mtlA"
                     /locus_tag="ACHOWS_004463"
     CDS             2506..4419
                     /gene="mtlA"
                     /locus_tag="ACHOWS_004463"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFQ0049944.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4784..5932
                     /gene="mtlD"
                     /locus_tag="ACHOWS_004464"
     CDS             4784..5932
                     /gene="mtlD"
                     /locus_tag="ACHOWS_004464"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0049945.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5932..6519
                     /gene="mtlR"
                     /locus_tag="ACHOWS_004465"
     CDS             5932..6519
                     /gene="mtlR"
                     /locus_tag="ACHOWS_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFQ0049946.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6530..6739)
                     /gene="yibT"
                     /locus_tag="ACHOWS_004466"
     CDS             complement(6530..6739)
                     /gene="yibT"
                     /locus_tag="ACHOWS_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFQ0049947.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            7023..7385
                     /gene="yibL"
                     /locus_tag="ACHOWS_004467"
     CDS             7023..7385
                     /gene="yibL"
                     /locus_tag="ACHOWS_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFQ0049948.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            7944..8627
                     /locus_tag="ACHOWS_004468"
     CDS             7944..8627
                     /locus_tag="ACHOWS_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFQ0049949.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            8671..>10322
                     /locus_tag="ACHOWS_004469"
     CDS             8671..>10322
                     /locus_tag="ACHOWS_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFQ0049950.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISVLVASG
                     LLSSFGALANNYDGQPTDYGDGSAGDGWVAIGKGAKANTFMNTSGASTALGYDAIAEG
                     EYSSAIGSKTLATGGASMAFGVSARAMGDRSVALGASSVANGDRSMAFGRYAKTNGYT
                     SLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDHANASKEYSMALGASSAASAANAI
                     AVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIALG
                     SGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALGQG
                     SYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDIST
                     TSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGLDN
                     NTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNTAN
                     ITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKIT"
BASE COUNT         2777 a         2475 c         2529 g         2541 t
ORIGIN      
        1 tcgatgaata acaacttaat tatatttaaa aacatattaa taactcactc tccaggagtt
       61 ttttaactta gtgttataat gaatacgata attaaaagtg aagagaaaaa tagttgtata
      121 catatattcc atgaacctac attgtgatct ataacttaaa tatttttaaa tgtgactgtc
      181 atccttcaga taataaagcc atcatcgcaa tagatggaaa agcactcctg ctcttttttg
      241 attagagccg tggcagagga acaactcata tcatcattgc gatctcgaca atgtatagtc
      301 tgatccttcg gcagatcaag acggataaaa aatccaatga gttcacactc atcccgaact
      361 tcttaacata atggatatta acgaaaaatc atcacagccg atgaaataat atgcaaaaat
      421 aattagtcct tacaaggagc aaagttccat aatctccccc ctccccctca atgatccaaa
      481 taaagataat gcatccaact ggtcattctc agcagcactt tccgcatcac cgaaacatgg
      541 ctgaaatggt cggagtaaac tgccacttgg gcgtagatgc cgtcgggtaa ggcatcgata
      601 tcatcgttag ggtccagttc aatggttccc agcacaccgt ccgtgccagg cacgaccgtt
      661 aatgattgca ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta
      721 gtcagtttgc cgtgaaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg
      781 ccgggtttca gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc
      841 tcggggatga aaaccatcac cggacgcaac ggcaaggcag ctgcgtatgt acctgggcgg
      901 atcagtacct gagtaacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaaggtta
      961 tattttgctt cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttaacc
     1021 atactgtcga gctggctctg gatctgcgcc tgctccgcca ccgagccttt caccagcgcg
     1081 tcctgcgcga ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cggattcacc
     1141 gccgcctggc tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct
     1201 gaaacctggg tggtgttggc ctgcgcttca gtgagctgcg cgcgcagcgt ctttatatta
     1261 tgcgtcgccg tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg
     1321 tcgagcttaa aaagcacctc gcccttttga ataagctgat tatttttgtc ggtgacttca
     1381 gtaacaattc ccgtcacctg tggcgtgatg gggatcgcta tcactgcctt ttgcgcggta
     1441 aaagtgtaag ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct
     1501 cccagcgccg cggtcgccag cgtccactga ttcaccggaa tgcggaagat tttaaagacc
     1561 gcccacgcca gcgccacgta agttaaaaca atcaatagat ccatatttag atctccggga
     1621 acgtggaata gtcagtcttt ttctcggcgg cgagttgatg ctccagccgg gaaattcggt
     1681 cagaaagtgc ggcgatttcc gggtcagtcg ctttctcctg cgacgcaaca tgtgactgca
     1741 taccccagcc acgctccggt tgatagagcg tcgcccagat ccacaggaac ggccagataa
     1801 catgcagagt aaacaggctc acccagcccg ccgtatgaat agcgtcggca tggggatgat
     1861 tgcgcttttt ggcaatcaga tatgggatgt catgtatcgc gatgattcca taaaaaatca
     1921 ccagaaagac gaagatcagc actcccaacg cgaaatagtt tagaaacata tctgcctcgg
     1981 attcacgttt atcagtgttg ttttttggct ggcagccaga agggagtcag gctgatattt
     2041 tgacaataat ccgggttcgc gattctcgcc ataacaccaa agaataattt ttagaggtga
     2101 tgagttgctt agttacataa cgattttatg acgaaggcat aacatgctgt agatcacatc
     2161 aggtgaacgc cgtaagaaaa tatcttgtga ttcagatcac aaagatccaa caaaccacca
     2221 aaacaaaaat gtgacattag tcacatttaa atgccatttt tagcgaaaat cgccgtcttg
     2281 ttgctttttt acacaagcat tttgtgatga gcatcacgtc aattacctct ctacccccta
     2341 tatttatgtg acccatatca cacaaaaggc cgtcgactgg acagttaacc gattcagtgc
     2401 cagatttcgc agtatctaca aggtccggct acctctgccg ccacattaac aaaaaacctc
     2461 gggcttccag cctgcgcgac agcaaacata agaaggggtg tttttatgtc atccgatatt
     2521 aagatcaaag tgcaaagctt tggtcgtttc ctcagcaaca tggtgatgcc aaatatcggc
     2581 gcgtttatcg cgtggggtat catcaccgcg ttatttattc caacagggtg gttaccgaac
     2641 gagacgctgg cgaagctggt cgggccgatg atcacttatc tcctgccgct gctgatcggt
     2701 tataccggtg gtaagctggt aggcggcgaa cgtggcggcg tagtcggtgc catcaccacc
     2761 atgggcgtta tcgtcggcgc agacatgccg atgttcctcg gttctatgat tgcaggtccg
     2821 ctgggcggct ggtgcattaa gcacttcgac cgctgggtag acggtaagat caaatccggt
     2881 tttgagatgc tggtgaataa cttctccgca ggcatcatcg ggatgatcct cgctattctg
     2941 gcattcctcg gcattggccc gattgttgaa gccctgtcca aaatgctggc tgcgggcgtt
     3001 aacttcatgg ttgtccatga catgctgccg ctggcgtcta tctttgttga accggcgaaa
     3061 atcctgttcc tcaacaacgc cattaaccac ggtatcttct cgccgctggg cattcagcag
     3121 tcccatgaat tgggtaaatc catcttcttc ctgattgaag ctaacccagg tccaggtatg
     3181 ggcgtgctgc tggcgtacat gttctttggt cgtggcagtg ctaaacaatc tgcgggcggt
     3241 gcggcaatca tccacttcct gggtggtatc cacgaaattt acttcccgta tgtgttgatg
     3301 aatccgcgtc tgatcctggc cgttatcctc ggcggtatga ctggcgtgtt cacgctgact
     3361 atcctgggcg gtggtctggt ttctccggca tctccgggtt ctatccttgc tgtactggcg
     3421 atgacaccaa aaggtgctta tttcgctaac atcgcaggtg tgtgtgcggc gatggctgtc
     3481 tccttcgttg tctctgccat tctgctgaaa accagcaaag tgaaagaaga agatgatatt
     3541 gaagcagcaa ctcgtcgtat gcaggacatg aaagctgagt ctaaaggtgc gtctccgctg
     3601 tctgcgggcg atgtgactaa cgacctgagc cacgtacgta aaatcatcgt tgcctgtgac
     3661 gccggtatgg gttccagtgc gatgggcgca ggtgtgctgc gtaagaaaat tcaggatgca
     3721 ggtctgtcgc agatttcggt cactaacagc gcgatcaaca acctgccgcc agatgtggac
     3781 ctggtcatca ctcaccgtga cctgaccgaa cgcgctatgc gccaggttcc gcaggcacag
     3841 catatttcgc tgaccaactt cctcgacagc ggcttgtaca ccagcctgac cgaacgtctg
     3901 gttgctgccc agcgccatac tgaaaacgaa gtaaaagtga aagacagtct gaaagacagc
     3961 tttgacgatt ccagtgctaa cctgttcaag ctaggcgcgg agaacatctt cctcggtcgc
     4021 aaagcggcca ccaaagaaga agcgattcgt tttgctggcg agcagctggt gaaaggcggt
     4081 tacgttgagc cagaatacgt tcaggcgatg ctggatcgtg aaaaactgac cccgacttat
     4141 ctgggtgagt ctatcgcggt gccacacggt acggttgaag caaaagatcg cgtactgaaa
     4201 acgggcgtgg tgttctgcca gtacccggaa ggcgtgcgct tcggtgaaga agaagatgac
     4261 attgcccgtc tggtgattgg tattgctgcc cgtaacaacg agcacattca ggttatcacc
     4321 agcctgacca atgcactgga tgatgaatcc gtcatcgagc gtctggcaca caccaccagc
     4381 gtggatgaag tgctggaact gctggcaggt cgtaagtaat acaatctcac cctctccaca
     4441 tggagaaggt ggggcttatt gcctgatgcg ctacgcttat caggcctaca aaacaaagca
     4501 cagtttgttg aatttgcacg ttcttgtagg ccggataagg cgtttacgcc gcatccggcg
     4561 ctgcccctct ccacatggag aagatgggct aattgcctga tgcgctacgc ttatcaggcc
     4621 tacaaaaaca aagcacaatt tgttgaattt gcacgttctt gtaggccgga taaggcgttt
     4681 acgccgcatc cggcgcggcc cctctccaca tggagagggt ttgggtgagg gaaaagcctc
     4741 accccagccc tctcgggtaa aaacattgat gaaggttaat actatgaaag cattacattt
     4801 tggcgcaggt aatatcggtc gtggctttat cggtaaactg ctggcagacg cgggtatcca
     4861 actgacgttt gccgatgtca atcaggtggt acttgatgcc ctgaatgccc gtcatagcta
     4921 tcaggttcat gtggtcggtg aaaccgagca ggtagatacc gtttccggcg tcaatgccgt
     4981 cagcagcatt ggtgatgatg tcgttgatct gattgctcag gttgatttag tcactaccgc
     5041 cgttggcccg gttgtgctgg aacgtattgc tccggcaatc gccaaagggc tggtgaaacg
     5101 taaagaacaa ggtaatgaat ccccgctgaa catcatcgct tgtgaaaaca tggtccgcgg
     5161 caccacacag ctgaaaggcc atgtgatgaa cgccctgcca gaagatgcca aagcgtgggt
     5221 agaagaacac gttggctttg tcgattccgc cgttgaccgc atcgtgccgc cttcggcttc
     5281 tgcaaccaac gatccgctgg aagtgacggt agaaacattc agcgaatgga ttgtcgataa
     5341 aacgcagttc aaaggcgcgc tgccaaacat ccctggcatg gaattaaccg acaatctgat
     5401 ggcatttgtc gaacgtaaac tcttcaccct gaatacaggt catgctataa ccgcgtacct
     5461 cggaaaactg gccggtcatc agaccattcg tgatgcgatt ctcgacgaga aaatccgcgc
     5521 ggtggtaaaa ggtgcgatgg aagaaagtgg tgcagtattg atcaagcgct acggctttga
     5581 cgcagacaaa catgcggcgt acatccggaa aatcctcggt cgtttcgaga acccgtacct
     5641 gaaagatgat gtagagcgcg taggccgtca gccgctgcgt aaactgagtg ctggcgaccg
     5701 tctaatcaag ccactgctcg gaacgctgga atatggtctg ccacataaaa acctgattga
     5761 aggtattgcc gctgcaatgc acttccgcag tgaagatgat ccgcaggctc aggaactggc
     5821 agcactgatc gctgacaaag gtccgcaggc ggcgctggca cagatttccg gtcttgatgc
     5881 caacagcgag gttgtatccg aggcggtaac cgcttataaa gcaatgcaat aatggtggac
     5941 caggcgcagg acaccctgcg cccgaataac agattgtcag atatgcaggc aacaatggaa
     6001 caaacccagg cctttgaaaa ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga
     6061 agctttctga tcaccgccgt cgagcttttg accgaggcgg taaatcttct ggtgcttcag
     6121 gtattccgca aagacgatta cgcggtgaag tatgctgtag aaccgttact cgacggcgac
     6181 ggtccgctgg gcgatctttc tgtgcgttta aaactcattt acgggttggg cgtcattaac
     6241 cgccaggaat acgaagatgc ggaactgctg atggcattgc gtgaagagct aaatcacgac
     6301 ggcaacgagt acgcgtttac cgacgacgaa atccttggac cctttggtga actgcattgc
     6361 gtggcggcgt taccaccgcc gccacagttt gaacctgcag actccagttt gtatgcaatg
     6421 caaattcagc gctatcaaca ggctgtgcga tcaacaatgg tcctttcact gacagagctg
     6481 atttccaaaa tcagcttaaa aaaagccttt caaaagtaag tactttcgct tacttctcct
     6541 ctacctgttt cggacgataa agctgacgat aatactccag acgttgtaga tataacggca
     6601 cgctttcatc aggtattccg gacggaatcg cgttacgggg aggtagtttt ttcagatact
     6661 cccggaacgc ctggctcgat gccatgaaat ctacggcttt atcgataaga agcggaacgt
     6721 tttcacctaa tttgcccatg atgatatctc cgtattaccc cgcgccggga atgcgcggcc
     6781 gccaatttta gtttagatcc cggtataaat gacattaagg aaaacgtgct caatcctcaa
     6841 taatcacctg cttctgttga ctatcttttt tctttaaatt tcttaattaa tcaataaatt
     6901 gcgtagtttt taagcatgtt aatgtttacg ttatagaact attgcaatgt catcagttgt
     6961 gaacaaagca cctggtcgcg catactaggg gctataaatt tatctttatc agaagccatc
     7021 acatgaaaga agtcgaaaaa aacgaaatca aacgtctcag cgatcgtctg gacgccatcc
     7081 gccaccagca ggccgatctg tcgctggttg aagccgcaga caaatatgcc gaactggaaa
     7141 aagagaaagc cactctggaa gcagaaattg ctcgcctgcg cgaagttcac agccagaaac
     7201 tcagtaagga agcacaaaag ctgatgaaga tgccattcca gcgcgcgatt accaaaaaag
     7261 agcaggctga tatgggcaag ctgaagaaaa gtgttcgcgg actggtcgtt gtgcacccaa
     7321 tgaccgcgct gggccgcgaa atgggcctgc aagagatgac agggttctca aagactgcgt
     7381 tttaagttcc tttctcgtcg ctgtaatcac agcctctcac catttactcg ctcctgctgg
     7441 cacatgcagg agcctgtttc agagatcgaa tatttataac gttcttaaca ttatatattc
     7501 aatctcctct attcttttta gcgcacatgc caggctaaat tctgaaaatc aggtacaagt
     7561 taaagaacga tgtatcaata ttaacaatga attgccgcat atcaatgctt gaaacataat
     7621 taattattta aatttttcta tcagcatcgc aaacttcgca ataaaacatc actcaactga
     7681 atatttacct ttccctgccc tgggccttta tccggtgaaa catttgacat tgattgacac
     7741 cgatcattaa aatccgcgcg attaatatta ttggctacaa accgaaattg tttgacactt
     7801 aataacatat tcgcacaacc gattaataca aaaattctct aacaaattta tttgtcggat
     7861 atgctccact ctaatcattc ataaaaaaaa tgattgtgcc gtaattatca actgtatcgg
     7921 gattatgaaa aggaattcat cctatgaacc taaagaaaac actgttaagc gtgttaataa
     7981 tagtgcctct ctgcttattg gcagggtgtg actatattga aaaagcgagt aaggtcgacg
     8041 acctcgttac acagcaagag ttgcaaaaaa gcaaaattga ggcgcttgag aaacaacagg
     8101 aactcgataa gcgaaagata gaacattttg aaaaacaaca aactactatc ataaatagta
     8161 ccaagacgct cgctggcgtg gtgaaggcgg ttaaagacaa acaggacgaa tttgtcttta
     8221 cagaatttaa cccggcacaa acccaatact tcattttaaa taacggctct gttggtttgg
     8281 caggccgggt gctggctatt gatgcagttg aaaatggcag cgttattcgt atttcgctgg
     8341 ttaatttatt aagcgttccc gtatcaaata ttggctttca tgcgacatgg ggcaatgaaa
     8401 ggcccaccga tgccaaagct ctggcaaagt ggcaacaatt acttttcaac accacactga
     8461 attccactct gcaattaatg cctggtcaat ggcaagacat taatctgaca ttaaaaggcg
     8521 tttcgcctaa taacctgaag tatctgaagt tgtccatcaa tatggccaat ctccagttcg
     8581 acactgttca atctgctgaa actcggcagc gaaaaaacaa aaaataaatg cagcttctgc
     8641 gcttatatca aggaatagag agcatcaata atgaacaaaa tttttaaagt tatctggaat
     8701 ccagcgacgg gcagttacag cgttgccagc gaaacggcga agagccgtgg taaaaaaagc
     8761 gggcgcagta agctgttaat ttctgtacta gttgcgagtg ggttgttgtc gtcgtttgga
     8821 gcgttagcta ataactacga cgggcagcca actgattatg gcgatggctc agcaggtgac
     8881 ggctgggttg ctatcggtaa aggggcaaaa gcaaatacct ttatgaacac tagtggcgcg
     8941 agtacagctt taggatatga cgcgatagcc gaaggtgagt acagttctgc catcgggtca
     9001 aaaacccttg caactggtgg agcatccatg gcgttcgggg ttagtgcaag agcaatgggt
     9061 gacagaagtg tcgcgctggg tgcatcgtca gtagcaaatg gcgatcgttc gatggctttt
     9121 ggtcgttacg caaagacgaa tggttataca tctcttgcta ttggggactc ctcccttgcc
     9181 gatggtgaaa aaactattgc gttaggaaat acggctaaag cttacgaaat tatgagcatc
     9241 gccctcggtg atcatgccaa tgcgtcaaaa gaatattcaa tggcgctggg tgcaagtagt
     9301 gccgcatctg cggctaacgc gattgcagta ggccgcaaca gtgctgcagc tggcgtagac
     9361 agcctcgcat tcggtagact atccgccgcc aacgccgcaa acgcgattgc tatgggtgca
     9421 gagagcaaag ctgctgaaaa tgcgacggca gttggtacta acgcggaggc aaatggactt
     9481 aatagtatcg cgttgggctc cggcagtatt gcagatgtcg acaacactat tgctctgggc
     9541 aatcaaagtc aggcagtagc ggctggcgca attgccatcg gtcaagggaa taaagctgac
     9601 ggcgcaaatg ctatcgcgct gggtaatggt agtattacag gtggtgtaaa tgctattgct
     9661 cttggacaag gcagttatgc cggtttagaa aatggcactg caattggtgc tcaagccagt
     9721 gctcagggga aaaattcagt tgctctgggt gctggttctg tagcgactga cgcggatact
     9781 gtttctgtgg gtaacacaac agctcagcga caaattgtca atatggcagc aggtgatatc
     9841 agcactacca gtactgatgc catcaatgga tcacagcttt atgctatcag taagtcagta
     9901 gcggacaatc ttggtggggg ggctaccgtc aatgcgcaag gcgtcgttac ttccccaaat
     9961 tacaggctga aaagtggtat tttcggcact gttggcgacg ccttaacggg cctggacaat
    10021 aatacgttac aatgggactc ccttaaaaag gcatatagtg cggcacatgg tacagatact
    10081 accagtacca tcaccaacgt taaagacggc gctatttctg ataccagtaa ggatgcggtt
    10141 aacggttctc agctgaaaac caccaacgat aacgtagcga ccaatactgc caatatcacc
    10201 accaacacta acagtatcaa taccctgacg gattccgttg gcgaccttaa agacgatgcc
    10261 ctgctgtgga atggcaccgc gttcagcgcc gcgcacggca ccgaagccac cagcaaaatc
    10321 ac
//
LOCUS       DAZKJR010000170        10297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKJR010000170 DAZKJR010000000
VERSION     DAZKJR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.171"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            129..1523
                     /gene="galP"
                     /locus_tag="ACHOWS_004470"
     CDS             129..1523
                     /gene="galP"
                     /locus_tag="ACHOWS_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFQ0049951.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1600..2097
                     /locus_tag="ACHOWS_004471"
     CDS             1600..2097
                     /locus_tag="ACHOWS_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFQ0049952.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2192..2899
                     /gene="endA"
                     /locus_tag="ACHOWS_004472"
     CDS             2192..2899
                     /gene="endA"
                     /locus_tag="ACHOWS_004472"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFQ0049953.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2979..3710
                     /gene="rsmE"
                     /locus_tag="ACHOWS_004473"
     CDS             2979..3710
                     /gene="rsmE"
                     /locus_tag="ACHOWS_004473"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0049954.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3723..4673
                     /gene="gshB"
                     /locus_tag="ACHOWS_004474"
     CDS             3723..4673
                     /gene="gshB"
                     /locus_tag="ACHOWS_004474"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFQ0049955.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4782..5345
                     /locus_tag="ACHOWS_004475"
     CDS             4782..5345
                     /locus_tag="ACHOWS_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFQ0049956.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5345..5761
                     /gene="ruvX"
                     /locus_tag="ACHOWS_004476"
     CDS             5345..5761
                     /gene="ruvX"
                     /locus_tag="ACHOWS_004476"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0049957.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5935..6915)
                     /locus_tag="ACHOWS_004477"
     CDS             complement(5935..6915)
                     /locus_tag="ACHOWS_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFQ0049958.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            6933..7637
                     /gene="yggS"
                     /locus_tag="ACHOWS_004478"
     CDS             6933..7637
                     /gene="yggS"
                     /locus_tag="ACHOWS_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFQ0049959.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLDVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7655..8221
                     /gene="yggT"
                     /locus_tag="ACHOWS_004479"
     CDS             7655..8221
                     /gene="yggT"
                     /locus_tag="ACHOWS_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFQ0049960.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8218..8508
                     /gene="yggU"
                     /locus_tag="ACHOWS_004480"
     CDS             8218..8508
                     /gene="yggU"
                     /locus_tag="ACHOWS_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFQ0049961.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8516..9109
                     /locus_tag="ACHOWS_004481"
     CDS             8516..9109
                     /locus_tag="ACHOWS_004481"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFQ0049962.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLIYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9102..10238
                     /gene="hemW"
                     /locus_tag="ACHOWS_004482"
     CDS             9102..10238
                     /gene="hemW"
                     /locus_tag="ACHOWS_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFQ0049963.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2414 a         2713 c         2715 g         2455 t
ORIGIN      
        1 gcacttaaat cataaccaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
       61 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata accatattgg
      121 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
      181 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
      241 gcgcactgcc gtttattgca gatgaattcc agattacttc gcacacgcaa gaatgggtcg
      301 taagctccat gatgttcggt gcggcagtcg gtgcggtggg cagtggctgg ctctccttta
      361 aactcgggcg caaaaagagc ctgatgatcg gcgcaattct gtttgttgcc ggttcactgt
      421 tctctgcggc tgcgccaaac gttgaagtac tgattctttc ccgcgttctg ctggggttgg
      481 cggtgggtgt ggcttcttat accgcaccgc tgtacctctc tgaaattgcg ccggaaaaaa
      541 ttcgcggcag catgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
      601 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
      661 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagt cctcgttggt
      721 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
      781 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
      841 gctgggcgct gtttaaagag aacagcaact tccgccgcgc ggtgttcctt ggcgtactgt
      901 tgcaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
      961 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacggtg atcgtcggcc
     1021 tgaccaacgt actcgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     1081 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     1141 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     1201 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     1261 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     1321 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     1381 tctgggtgta tgcggctctg aacgtactgt ttatcttgct gacattgtgg ctggtaccgg
     1441 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     1501 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccacc
     1561 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     1621 atcgccatcc agcaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     1681 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     1741 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     1801 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     1861 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     1921 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     1981 ccctaccgct gcaagtgcca ggagcatcag cttaccgtac gccgccataa tcgcgtagtt
     2041 cgtggcgagg ccgtctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     2101 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     2161 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     2221 ggtactgagc gcagcatttt ccggcccggc gttggccgaa ggtatcaata gtttttctca
     2281 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     2341 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     2401 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     2461 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ccaaagatcc
     2521 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gtgaggtgaa
     2581 tggcgatcgc ggcaacttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     2641 atgcgccatg aaggtcgatt tcaaagaaaa agccgccgaa ccaccagcgc gtgcacgcgg
     2701 tgccattgcg cgcacctact tctatatgcg tgaccaatac aacctgacac tctctcgcca
     2761 gcaaacgcaa ctgttcaacg catgggacaa gatgtatccg gttaccgact gggagtgcga
     2821 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     2881 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     2941 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     3001 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     3061 tcgggcgcgt actgcgtatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     3121 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtactgg
     3181 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     3241 gtggtgaaaa aatggaattt acgatccaga aatcgatcga actcggtgta agcctcatta
     3301 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     3361 tccagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     3421 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     3481 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccgtta ccggttgaac
     3541 gtgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     3601 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     3661 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     3721 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     3781 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     3841 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     3901 agcagaacta cgaagagtgg ttttcattcg tcggtgaaca ggatctgccg ctggccgatc
     3961 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     4021 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     4081 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     4141 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     4201 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     4261 tcggcgtgat tgccgaaacc ctgactgagc atggcactcg ctactgcatg gcgcaaaatt
     4321 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     4381 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     4441 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     4501 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcattggc gaccgtctga
     4561 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     4621 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     4681 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     4741 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     4801 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     4861 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     4921 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     4981 gtctggataa accggttatg ctcggtggtc cgctggctga agatcgcggg tttattttgc
     5041 atactccgcc ctctaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     5101 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     5161 ctctgggcta tgcctcctgg gagaaaggtc aactggaaca agaaattctc gataacgcgt
     5221 ggctaacggc cccggcggat ctgaatattc tgttcaaaac accgattgcc gaccgctggc
     5281 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcagggcacg
     5341 cctgatgagt ggaaccttac tcgccttcga cttcggtacc aaaagcattg gtgtagcggt
     5401 cggccaacgc attaccggca ccgctcgccc attgcctgca attaaagcac aggacggtac
     5461 gccggactgg aacattatcg agcgtttact gaaagagtgg cagcctgacg aaatcatcgt
     5521 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgtaaatt
     5581 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     5641 caccgtggag gcacgttccg gtctgtttga acagggcggc tatcgggcgc tcaacaaagg
     5701 caaagtcgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
     5761 aggcgattta ctagcgcaca gatttctgac aatgtgctcg ttgtttcatg ttggatgcgg
     5821 cgtaaacgcc ttatccaacc tacagtgagt gcgaattcaa agttacgctt cccttgtagg
     5881 cccgataagc ttgcgcatcg ggcatattta caattcgtat cagacacagc aacgtcacaa
     5941 gcgcccttct ttcacccgct gctgataact ctgctgaaac gttatcatcc ccacctgctg
     6001 cccggtttga ataacatgcg gtaactggtg ggttttccct tcacgaatca aattccccac
     6061 cgcgggtgtg ttaatcagca attcaaacaa cgccacgcgt ccttcctgtt tatccacttc
     6121 cagcttttgt gacaacaccg cccgtaaact acctgccagt tgattacgca cgggatcttt
     6181 ttcctgcgcc ggaaatgaat ctaccagtcg ctcaactgcc tgcgccgcac cacgcgtatg
     6241 taatgttgcc agcaccaaat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
     6301 gctgtcacgc agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
     6361 tcccgatgcg aacgtcatac aatgcaaacc gatttcccgc tgctggatca aacatcgctg
     6421 gcttgcatag agatattcga caggatcttc cagcgtcaga atatgcgcat cggcatgttg
     6481 attgagatag ccaaccatcg ccgccagcgt ggtagatttg ccgctccccg tcgcccccgt
     6541 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gcgcaccaag
     6601 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
     6661 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacag caaaatccaa
     6721 ctgaccattc tccagcaata ttgtccgctg atcgtcatcc agccactccc gcagtagctc
     6781 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattcgc ccgcgaatgc gccatcgtgc
     6841 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
     6901 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
     6961 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
     7021 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
     7081 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
     7141 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
     7201 gccgcctggt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
     7261 cccgcctcaa cgatcagcgc ccggcagaac ttccccctct tgacgtactg attcaaatta
     7321 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
     7381 ctgcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
     7441 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
     7501 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
     7561 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
     7621 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
     7681 aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc agtgggctca
     7741 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtaacgcagc caattatcgg
     7801 gccactgcgc cgcgttattc cggcaatggg acccattgac agcgcctcgc tgctggttgc
     7861 ctatattctc agttttatca aagccatcgt gctgtttaaa gtggtgacct tcctgccaat
     7921 catctggatt gccggtttac tgattctgct gaaaaccatc ggcctgctga ttttctgggt
     7981 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagtccga ttgaatacgt
     8041 gctgattcag cttgccgatc cgctgctgcg cccgattcgc cgcctgctac cggcaatggg
     8101 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca atatgggtgt
     8161 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
     8221 agtgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca gccgaaagcc
     8281 agccgtgatt ctattgtcgg tttacatggc gacgaggtaa aagtcgccat taccgcgccg
     8341 ccggttgacg gccaggccaa cagccatctg gtgaagtttc tcggtaagca attccgggtt
     8401 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
     8461 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
     8521 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
     8581 cgacttcggt cttgatatcg tggcccaaac agacctcggc gtagattccg ctgaagaaac
     8641 cggcctgacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
     8701 tttaccggca attgccgacg actccggtct ggcggtagat gcgctgggtg gcgcgccggg
     8761 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaacc tgcaaaaact
     8821 gctggaaaca atgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
     8881 gatatatctg cgtcacgcgg aagatccgac tccgctggtg tgccacggta gctggccggg
     8941 cgtgattact cgtgaaccag cgggtactgg tggctttggt tatgatccaa ttttcttcgt
     9001 tccttctgaa gggaaaaccg ctgccgaact gacccgcgaa gaaaagagcg ccatttccca
     9061 ccgtggtcag gcattgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
     9121 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
     9181 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg cttaacgatc
     9241 tggacaacga tgtggcttac gctcagggcc gtgaagtgca gacaatcttt attggcggtg
     9301 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctggacggc gtgcgtgcgc
     9361 gtttgccgct ggcagcggat gcagaaatta cgatggaagc gaacccaggc acggtagaag
     9421 cggatcgctt tgtcgattat cagcgtgctg gtgtgaaccg catctctatt ggtgtgcaga
     9481 gttttagcga agaaaagctg aaacgactcg ggcgtattca tggcccgcaa gaagcgaaac
     9541 gagcggcaaa gctggcgagc ggtttagggt tacgtagttt taaccttgat ttgatgcatg
     9601 ggctgccgga tcaatcactg gaagaggcgc taggcgatct gcgccaggct attgaactga
     9661 atccgccaca tctttcctgg tatcaactga ccatcgaacc taatacgctg tttggctcgc
     9721 gccctcctgt actgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
     9781 tattaaccgc agcgggctat cagcagtatg aaacatccgc ttacgccaaa cccggttatc
     9841 agtgccagca caatctcaac tactggcgat ttggcgacta catcggtatt ggctgcggcg
     9901 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccaccaaa acgcgtcatc
     9961 cgcgtggttt tatgcaggga agatatctgg aaagccagcg tgatgtcgaa gctgcagata
    10021 aaccgtttga gttctttatg aatcgcttcc gtctgcttga ggccgcgccg cgcgtggagt
    10081 ttagtcagta tactggcctt tctgaagaag ttattcgctc tcagttagac gaggctatcg
    10141 ctcagggtta tctcacagaa tgtgcggatt actggcagat aaccgagcat ggtaaattat
    10201 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    10261 attaatcact tcttttaccc cccccgttaa ttaactt
//
LOCUS       DAZKJR010000171        10246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKJR010000171 DAZKJR010000000
VERSION     DAZKJR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.172"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..1081
                     /locus_tag="ACHOWS_004483"
     CDS             173..1081
                     /locus_tag="ACHOWS_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFQ0049964.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1127..3118)
                     /gene="tkt"
                     /locus_tag="ACHOWS_004484"
     CDS             complement(1127..3118)
                     /gene="tkt"
                     /locus_tag="ACHOWS_004484"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0049965.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3396..4154
                     /gene="loiP"
                     /locus_tag="ACHOWS_004485"
                     /gene_synonym="yggG"
     CDS             3396..4154
                     /gene="loiP"
                     /locus_tag="ACHOWS_004485"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFQ0049966.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4202..5122)
                     /gene="speB"
                     /locus_tag="ACHOWS_004486"
     CDS             complement(4202..5122)
                     /gene="speB"
                     /locus_tag="ACHOWS_004486"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFQ0049967.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5258..5989)
                     /locus_tag="ACHOWS_004487"
     CDS             complement(5258..5989)
                     /locus_tag="ACHOWS_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049968.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6135..8111)
                     /gene="speA"
                     /locus_tag="ACHOWS_004488"
     CDS             complement(6135..8111)
                     /gene="speA"
                     /locus_tag="ACHOWS_004488"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFQ0049969.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8120..8251)
                     /gene="yqgB"
                     /locus_tag="ACHOWS_004489"
     CDS             complement(8120..8251)
                     /gene="yqgB"
                     /locus_tag="ACHOWS_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFQ0049970.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8345..8488)
                     /gene="yqgG"
                     /locus_tag="ACHOWS_004490"
     CDS             complement(8345..8488)
                     /gene="yqgG"
                     /locus_tag="ACHOWS_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFQ0049971.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8599..8850)
                     /gene="yqgD"
                     /locus_tag="ACHOWS_004491"
     CDS             complement(8599..8850)
                     /gene="yqgD"
                     /locus_tag="ACHOWS_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFQ0049972.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8906..10060
                     /gene="metK"
                     /locus_tag="ACHOWS_004492"
     CDS             8906..10060
                     /gene="metK"
                     /locus_tag="ACHOWS_004492"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0049973.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2541 a         2737 c         2591 g         2377 t
ORIGIN      
        1 ttgttgtatt tgtgatacat atcgccattt atttattgaa tgtccgaaag catacttttc
       61 taatgcaatg ttttttcgct ataccccttt atagattctg aattctcacc tgtcggctat
      121 tttctcctca cctcatgtga catattcccc actttactca aggaagattc atatgaaagc
      181 acctgtttca ataacctcat ttcgcccaca aaaatctatt gctatcgtca ttggcgtgct
      241 tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat
      301 tttgctgcgc tacggaaaaa tcgtaaaagt tgccgagccg ggtctgggtt ttaagatccc
      361 ttttatggaa agcgtggaga aaatttccac gcgtaatcag gctgtggttt atcagggatt
      421 acaggcctac agccgcgacc agcaaccggc acaaatgaca gtatcagtca gcttccacat
      481 taaaccttca gaagcaggcg cggtttatac cacctacaac accattgaag cattaaaaga
      541 tcgtctgatt gtccgtcagt tgccaactca actggagaac gttttcggcc aatataccgc
      601 catcagcgcc gtacaggatc gtacaaaact ggttcaggat ctacaaaatg caatgcgtaa
      661 agccgttgtt ggcccggtgg tgattgatgg tgtccagatt gaaaatattg atttttctga
      721 cgcttatgaa aaatcgattg aagatcgaat gaaagcagaa gtcgccattg ccacacgtaa
      781 gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg gtaactcagg cccaggcaga
      841 agcagacagt aaactggcgg cagccaaagc agaagctgag actattcgcg taagaggcgc
      901 ggcagaggct gaaactattc gtttaaaaag tgcggctgaa gcagaagcga tccgcctgcg
      961 cggtgaagct ctgcgggata atcccggact tgttgccctc accactgctg agcgttggga
     1021 cggtaagcta cccgatacca tgatccccgg cagtacagtt ccgtttatct ctactaagta
     1081 aaaaaagggc cgctttcgcg gcccttctca caaagcatct taccgattac agcagcgctt
     1141 tcgctttcgc aacaacgttg tcaacggtga aaccgaactc ttcaaacagt tgctctgcag
     1201 gagcagattc accgaaggtg gtcatgccga cgatagcacc gttcaggcca acatacttgt
     1261 accagtagtc agcaataccc gcttccaccg caacacgagc ggtaaccgct ttcggcagta
     1321 cggattcacg gtaagccgca tcctgcttgt cgaacgcgtc ggtagacggc atggaaacca
     1381 cgcgcgcctt cacgccttcg gcagtcagtt tttcgtaggc ggcaacagcc agttcaactt
     1441 ccgaaccggt cgcgatgaag atcagttccg gctgaccggc acagtctttc agcacataac
     1501 caccgcgcgc gatgtttgcc agctgctctt cagtacgctc ctgctgcgcc aggttctgac
     1561 gggagaggat cagcgcggtc gggccgtcct gacgctcaac gccgtatttc cacgcgaccg
     1621 cggattcaac ctggtcacat ggacgccatg tggacatgtt cggggtcacg cgcagagaag
     1681 cgacctgctc taccggctgg tgagtcgggc catcttcgcc cagaccgatg gagtcgtggg
     1741 tgtaaaccat cacctgacgc tgtttcatca gcgcagccat acgtacggcg ttacgtgcgt
     1801 attccacgaa catcaggaag gtggaggtgt acggcaggaa accaccgtgc aaggagatac
     1861 cgttagcaat cgcggtcata ccgaactcgc gaacaccgta gtggatgtag ttacccgcag
     1921 catcttcgtt gattgcttta gaaccagacc acagggtcag gttagacggt gccaggtcag
     1981 cagagccgcc gaggaattcc ggcaacagcg ggccgaaagc ttcgatagca ttctgagacg
     2041 ctttacggct ggcgattttc gccggattag cctgcagttt agcgatgaat tctttcgctt
     2101 tggcatcgaa gtcagacggc atttcgcctt tcatacggcg ggtaaattca gcggcttcct
     2161 gcggataagc tttggcgtaa gccgcgaatt tctcgttcca tgcggattct ttcgcctggc
     2221 ctgcttcttt cgcatcccac tgagcataga tttcagacgg gatttcgaac ggtgcatatt
     2281 tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc gcccagcggc gcaccgtggg
     2341 agtcgtgcgt accggctttg ttcggggaac cgaaaccgat gatggttttg cacatcagca
     2401 gggacggttt gtcagtcact gcgcgcgctt cttctactgc gcgtttgatg gatgccgcgt
     2461 catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc ttcgaaacgc attgcggtgt
     2521 cgtcggtaaa ccagccttca acgtgaccat cgatagaaat accgttgtca tcgtagaacg
     2581 caatcagttt acccagcttc agcgtacccg ccagagagca aacttcgtgg gagatgcctt
     2641 ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg gtcgacgatg tcgtggcccg
     2701 gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc cataccgact gcgttggcaa
     2761 taccctgacc cagcggacct gtagtggttt ccacgccagc ggtgtaaccc acttccggat
     2821 ggcccggagt tttagagtgc agctgacgga agtttttcag ttcttccatc ggcagatcgt
     2881 aaccggtgag gtgcagcagg ctgtagatca gcatggagcc gtggccgttg gacagcacga
     2941 agcggtcacg gtcagcccag gacggattct gcgggttgtg tttcaggaaa tcacgccaca
     3001 ggacttcggc aatgtcagcc atacccatcg gggcgcccgg gtgaccggat ttggctttct
     3061 gtactgcgtc catgctcagc gcacgaatag cattggcaag ctctttacgt gaggacattt
     3121 tgactccaga tcggatgatg aagggcacgc ccttaacgac ttgacgacag cgcgttttgg
     3181 gctacgccgg aaaatttgcc aacaatttac cgcaagccgc gcgtcatgta catggaacat
     3241 ccttttgccg cttcagaaat ctctggatca tgctcgcatg ttgcgcaatc tgctcgcccg
     3301 tccgctgcgc ttttccttat actgagactg agcgtcgatt cacctgcaaa cggcgcattt
     3361 ttagaataat cctgaccttg tgcggaagag aaaacatgaa aattcgcgcc ttattggtag
     3421 caatgagcgt ggcaacggta ctgaccggtt gccagaatat ggactccaac ggactgctct
     3481 catcaggagc ggaagctttt caggcttaca gtttgagtga tgcgcaggtg aaaaccctga
     3541 gcgatcaggc atgtcaggag atggacagca aggcgacgat tgcgccagcc aatagcgaat
     3601 acgctaaacg tctgacaact attgccaatg cgctaggcaa caatatcaac ggtcagccgg
     3661 tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt tgcaatggct aacggctgta
     3721 tccgcgtcta tagcgggctg atggatatga tgacggataa cgaagtcgaa gcggtgatcg
     3781 gtcacgaaat ggggcacgtg gcgttaggcc atgtgaagaa agggatgcag gtggcacttg
     3841 gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg gattgtcggc agtttatctc
     3901 aatcacaact cggtgatctg ggcgagaaat tagttaattc gcaattctcc cagcgccagg
     3961 aagcagaagc cgatgattat tcttacgatc ttctgcgcca acgcggcatc agcccggcag
     4021 gtcttgccac cagttttgaa aaactggcga agctggaaga aggccgtcaa agctcaatgt
     4081 ttgacgacca tccagcatcc gccgaacgcg cccagcatat tcgcgatcgt atgagcgcgg
     4141 atggagttaa gtaagcattt tattacgccg gatttgacgc ataacgcatc ttatccggcg
     4201 cttactcgcc ctttttcgct gcctgaatat acagcatctc cagcgccagc gtcgccgcag
     4261 ccagagcggt gatttccgac tgatcgtatg ccggagccac ttccactacg tccatcccaa
     4321 caatgttgag atctttcagg ccgcgtacca gtttaatagc gcgatcggag gtcaggccgc
     4381 caattactgg cgtaccggtt cccggcgcaa atgcaggatc caggcagtcg atatcaaagg
     4441 tcaggtaaac cggcatatca cccacaatct gtttcacttg ggcgataacg tcatccacgc
     4501 tgcgatcgtt cacctggcag gcgtccagca cggtaaagcc gttgtcttta tcgaactcag
     4561 tacgaatacc aatctgcacg gaatgattcg ggtcgatcag accttctttc ggcgcggtat
     4621 agaacatggt gccgtggtca aattcacaac cgttcgcata ggtgtcggtg tgggcgtcaa
     4681 agtgtaccag cgccattttg ccgaaatgct tcgcatgagc acgcagcagc ggcagcgtaa
     4741 caaagtggtc accaccgaag gagagcatac gcttaccggc agccagcagc ttctcggcgt
     4801 gcgcctgcag cttctcgctc atctcacggg catctccgaa ggcatatacc agatcgccgc
     4861 agtccacgac gttcaggcgc tcacgcatgt cgaaattcca cgggaagcga ttgtgttccc
     4921 aggccagatt cgtcgaaacc tgacggatcg ctgccggacc gtggcgacca cccgcacgac
     4981 cagaagtggc catatcgaac ggcacgccag taatcaccca gtcagcatcg ctgtcatatg
     5041 gctggaagtt catcggcagg cgtaaaaaac caaaggcgtt ggaaaccagt gagttatcgt
     5101 attgatgacc taaggtgctc atggatggac tcctattaca aagtcgatat aaaaaaaccc
     5161 ttccgcgtcg ttaggcccga cgaggaaggg ttggatttgc gaaacacaat tttgtgctaa
     5221 ttatcgcctt aaatgggcga ggcatcaagc gaggttgcta tttccgacac ggtggcggcg
     5281 catagccatt gttatctgcc ggaaaggctt gtccagagca cggcggcagc gcgttttgcg
     5341 ccgggccttg taattcaaag gcggcaaagc cgattacacc ggtattacgc acatcgcctt
     5401 gcgccgaatt ggctgcgtag gaatcagcaa cgttggcgaa ctgaaaagcc gcttcggtat
     5461 gtttatcttt gcggaatcct ttaatcgcta acgaatctcc ggcattcaca atatagccgt
     5521 tgttattcag cgaaccttgc ttgccgttca gcacatccag gccatcaacc gtagcaacga
     5581 tctcgtagtt ggtattacga ctgtaattac ggatatataa ttgataattc attccgactc
     5641 tggcatggac aatatagcgt ggttcattat tggatgaatc ataaacccgc gtaataggta
     5701 gagaattaaa attaccatcg cgaatagcat attccagttc gttaatacga atgctataaa
     5761 cgcggtcata gccggaagga acagtagtcg aataactaat aacgatcatc tctgccggat
     5821 cgcgccagac tcgttcagca gatacgccct gaaccgatga gtgtacatcc ccaccccagg
     5881 cggtgccgac attgcctgat gcactgctat tatactgcgc attactggaa caaccggcca
     5941 gcagcacggc taatgctatc aacgcgctac gtactttcca ttttttcata accgttccct
     6001 ctgcatttaa ttaatgcagc gattgtaggg ggattattta ataagaaaag cagctaaatt
     6061 atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc gacccgccag
     6121 cggaataatc ttaattattc atcttcaaga taggtataac cgtacaaacc tgcctcgaac
     6181 tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac ttgatcacgg
     6241 aactgggtta acaacgtttt cggatcgagc tgtacatatt gcagcatatc cgccacggta
     6301 tcgccttcgt cagacagttc tacttctacg ctgccgtcag ggaagacgaa cacgtcaacc
     6361 gcttcggtat caccgaacag gttgtgcatg ttgccgagga tctcctgata tgcgccgacc
     6421 atgaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg cattgtcgtg
     6481 gcaataccat caccatcgat gtagtgatca atagcaccgt cagaatcaca ggtaatatcc
     6541 agcagcacag cgcggcgctc cggcacttga tccaaccctt ccagcggcag aaccgggaac
     6601 aactggtcga tcccccatgc gtccggcatc gactggaaca gcgagaagtt gacgtacatt
     6661 ttgtcagcca tacgttcctg cagctcgtcg ataatcggac gatgcgcacg gttttgcgga
     6721 tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc ccatgcacgt
     6781 tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tatcgtgcag atccatctgg
     6841 ctgtcgtgta accattcacg cagagaacga cgagttcccg gttcgtgcat ctcctgccag
     6901 gtttcccaca tgctttgcag ggcgcgcggt gcgtcttctg caggcgcggt cggcaccgtg
     6961 tattcgttac gttccacgcc gatgatattc gacaccagca cggtgtgatg cgcagtcacc
     7021 gcacgacccg actcggtgat taccgtcgga tgtggcagac cattttcttc acacgcatca
     7081 ccaatcgccc agatgatgtt gttggcgtat tcattgaggc cgtagttcac cgaacagtcg
     7141 gactgcgaac gggtgccttc atagtccacg cccagaccgc cgccaacgtc gaagcactga
     7201 atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg aacgcctgtc
     7261 gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagcag ttgcaggctg
     7321 tcgagacgcc cggcttcacg cagcgtttca accagttgca gtacctgagt cgccgccagg
     7381 ccgaacttcg atttttcccc gccagaggac tgccatttac ccgagccctg cgaagccaga
     7441 cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc cagcacaatg
     7501 gcaatttctg acatcttctc aatgaccaga tagaccttgt ggcccatctt ctcgccaatt
     7561 aatgccaggc ggatatattc gcggtcctta taaccgttgc agacgatgac gctacgggtc
     7621 atgccagcat gtgccagcac cgccatcaac tccgctttgg aaccggcttc caggcccagc
     7681 ggttcgcccg aatgaatcag ggactcaatc acgcggcggt gctggttaac tttgatcgga
     7741 taaacgagga agtaatcgcc gttatagccg taggattccc gcgcacgttt gaacgcggcg
     7801 ttaatagaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc aggcagacgc
     7861 tggccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc ttccgggacg
     7921 tccgggtccg ggcacacgct aatgtggccc agctcgttaa cgtcatagta gttattgccc
     7981 caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct cattgcaacc
     8041 tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc catagacatg
     8101 tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta acgactatcg
     8161 cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt gaatgctgac
     8221 gctccaactg cgcaaccggt ttctttttca taacattatt aagcacataa ccgaacgtaa
     8281 gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg cccttgttca
     8341 gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg gtataacatc
     8401 ggcaggtatg caaagcagag atgcagagtg cggggaacga atcttcacca gaacggtgag
     8461 acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc cgcctgttaa
     8521 gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa cacgaaagaa
     8581 acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa tggcagaaaa
     8641 ttgcaaaaga taaatacgca gaatgccagc attgtcagga aaaatttcca gccacgtttt
     8701 taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt gacctaaaat
     8761 agccatccag atgttaatcc atccataccg attaacactc agactgccag tgtttttaac
     8821 ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt tgagctaacc
     8881 aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg agtccgtctc
     8941 tgaagggcat cctgacaaaa ttgctgacca aatttctgat gccgttttag acgcgatcct
     9001 cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg gcatggtttt
     9061 agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca cccgtaacac
     9121 cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact cctgtgcggt
     9181 tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc gtgccgatcc
     9241 gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacca atgaaaccga
     9301 cgtgctgatg ccagcgccta tcacctatgc acaccgtctg gtacagcgtc aggctgaagt
     9361 gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg tgactttcca
     9421 gtatgacgac ggcaaaatcg ttggtatcga tgctgtcgtg ctttccactc agcactctga
     9481 agaaatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca agccgattct
     9541 gccggctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg gtcgttttgt
     9601 tatcggtggc ccgatgggtg actgcggtct gactggtcgt aaaattatcg ttgataccta
     9661 cggcggcatg gcacgtcacg gtggcggtgc attctccggt aaagatccat caaaagtgga
     9721 ccgttccgca gcgtacgcag cacgttatgt cgcgaaaaac atcgtggctg ctggcctggc
     9781 cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga cttccatcat
     9841 ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc tggtacgtga
     9901 attcttcgac ctgcgcccat acggtctgat tcagatgctg gatctgctgc acccgatcta
     9961 caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg aaaaaaccga
    10021 caaagcgcag ctgctgcgtg atgctgccgg tctgaagtaa tctttcttca cctgctttca
    10081 aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggacag gcattcacgc
    10141 cgcatccggc aaaaaaaccg cccgcacaat aacatcattc ctcctcaaca cgttttaccg
    10201 caaattacca tcactactga aaccgattac accaaccacg ccagcc
//
LOCUS       DAZKJR010000172        10183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKJR010000172 DAZKJR010000000
VERSION     DAZKJR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.173"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            314..1291
                     /gene="glaH"
                     /locus_tag="ACHOWS_004493"
     CDS             314..1291
                     /gene="glaH"
                     /locus_tag="ACHOWS_004493"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFQ0049974.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            1311..2579
                     /gene="lhgO"
                     /locus_tag="ACHOWS_004494"
     CDS             1311..2579
                     /gene="lhgO"
                     /locus_tag="ACHOWS_004494"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFQ0049975.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALQEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2602..4050
                     /gene="gabD"
                     /locus_tag="ACHOWS_004495"
     CDS             2602..4050
                     /gene="gabD"
                     /locus_tag="ACHOWS_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFQ0049976.1"
                     /translation="MKPNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4064..5344
                     /gene="gabT"
                     /locus_tag="ACHOWS_004496"
     CDS             4064..5344
                     /gene="gabT"
                     /locus_tag="ACHOWS_004496"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFQ0049977.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYI
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGHKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5582..6982
                     /gene="gabP"
                     /locus_tag="ACHOWS_004497"
     CDS             5582..6982
                     /gene="gabP"
                     /locus_tag="ACHOWS_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFQ0049978.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7003..7665
                     /gene="csiR"
                     /locus_tag="ACHOWS_004498"
     CDS             7003..7665
                     /gene="csiR"
                     /locus_tag="ACHOWS_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFQ0049979.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKRDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7666..8115)
                     /gene="kbp"
                     /locus_tag="ACHOWS_004499"
     CDS             complement(7666..8115)
                     /gene="kbp"
                     /locus_tag="ACHOWS_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFQ0049980.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8199..8357)
                     /gene="yqaE"
                     /locus_tag="ACHOWS_004500"
     CDS             complement(8199..8357)
                     /gene="yqaE"
                     /locus_tag="ACHOWS_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFQ0049981.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8540..8839
                     /gene="ygaV"
                     /locus_tag="ACHOWS_004501"
     CDS             8540..8839
                     /gene="ygaV"
                     /locus_tag="ACHOWS_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0049982.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8849..9373
                     /gene="ygaP"
                     /locus_tag="ACHOWS_004502"
     CDS             8849..9373
                     /gene="ygaP"
                     /locus_tag="ACHOWS_004502"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFQ0049983.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9420..9824)
                     /gene="stpA"
                     /locus_tag="ACHOWS_004503"
     CDS             complement(9420..9824)
                     /gene="stpA"
                     /locus_tag="ACHOWS_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFQ0049984.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2379 a         2598 c         2746 g         2460 t
ORIGIN      
        1 aaaacggggc agataatatg tttagtttac taacggtcat tttgcagtga agccatttac
       61 cgttttttat cgaccagata atctgttctc tgatggtaat ccacccctaa cctgttgctt
      121 tagttattca tttcctgtct cactttgcct taataaccta cgtcaaatgt taccaatttg
      181 ttgtttttga tcgcaataag aaaacaatat gtaactttta tgcgcatttt tcagaaatgt
      241 agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac atctcataag
      301 aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg attcaggcca
      361 ggactatagc ggattcactc tcatcccgtc ggcgcaatct ccgcgtctgc tggaactcac
      421 cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc
      481 gctggagtac aaatcttttc tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc
      541 gaaccagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt
      601 gatcaatgcg gtgggtgtcg atgatgtgaa gcaagcggat gagatggtga agctggcaac
      661 ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc
      721 gcgattcgtg gtgaaaaatg ttgataactc agacagctat ctgcgtcagc cgcaccgcgt
      781 aatggagctg cacaacgacg gcacctacgt cgaagagatc accgactatg tgctgatgat
      841 gaaaatcgac gagcagaaca tgcagggtgg aaattcgttg ctgctgcatc tcgatgactg
      901 ggaacatctg gaccactatt tccgccaccc gctggcgcgt cgcccgatgc gctttgccgc
      961 gcctccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg ttgatcaaca
     1021 gggtcgcccg gtgatgcgtt atatcgacca gttcgtccag ccaaaagact tcgaagaagg
     1081 cgtgtggctg agtgagcttt ccgatgccat tgaaaccagc aaaggcattc tttctgtgcc
     1141 cgttcccgtt ggcaaattcc tgttgattaa caatctattc tggctgcacg gtcgcgaccg
     1201 ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct atttcgctta
     1261 cgccactaac cactaccaga cgcatcagta agcgcaaagg aattgagcgg atgtatgatt
     1321 ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg attgatgttt
     1381 atccggatgc ccgcattgcg ttgcaggaaa aagagtccgg cccggcctgt caccagacgg
     1441 gccacaacag tggcgtgatc catgccgggg tctattacac gcccggtagc ctgaaggcac
     1501 agttttgcct ggcgggaaac cgcgccacca aagccttttg cgatcaaaac ggcattcgct
     1561 atgacaactg cggcaagatg ctggtcgcca cctctgaact cgaaatggaa cggatgcgcg
     1621 cgttgtggga acgcacggca gcgaacggta tcgagcgcga gtggttaaac gccgaggaac
     1681 tgcgcgagcg cgaaccgaat atcaccgggc tcggcggcat ttttgtgccg tccagcggca
     1741 ttgtcagcta ccgcgaagtg acggcggcga tggcaaaaat cttccaggcc agaggcggcg
     1801 agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc gtggtgatac
     1861 gtacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc gggctgatgg
     1921 ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc ccgttccgtg
     1981 gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg atttacccca
     2041 ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc gatggcagcg
     2101 tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgt aagcgcgact
     2161 tctcatttag cgacacgctg gagattttgg gctcgtcggg gattcgccgg gtgctgcaaa
     2221 accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc ggctatctgc
     2281 ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc tggcccgctg
     2341 gtgtgcgggc gcaggcggta tcgccggacg gcaagctgat tgacgatttt ctgtttgtca
     2401 ccaccccgcg cacgatccac acctgcaatg cgccctcccc ggcagcgaca tcagcaattc
     2461 ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag agtaaccccg
     2521 gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt aatcaataac
     2581 ctttgaaaac aggacgtaac aatgaaacct aacgacagta acttattccg ccagcaggcg
     2641 ttgattaacg gggaatggct ggacgccaac aatggcgagg tcatcgacgt caccaatccg
     2701 gcgaacggcg acaagctggg tagcgtaccc aaaatgggcg ctgatgaaac ccgcgccgct
     2761 atcgacgccg ccaaccgcgc tctgcccgcc tggcgtgcgc tcaccgccaa agaacgcgcc
     2821 accattctgc gcaactggtt caatttgatg atggagcatc aggacgattt agcgcgcctg
     2881 atgaccctag aacagggcaa accgctggcc gaagcgaaag gcgaaattag ctacgccgcc
     2941 tcctttattg agtggtttgc cgaagaaggc aaacgcattt atggcgacac cattcctggt
     3001 catcaggccg ataaacgcct gattgttatc aagcagccga ttggcgtcac cgcggctatc
     3061 acgccgtgga acttcccggc ggcgatgatt acccgcaaag ccggtccggc gctggcggca
     3121 ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct ggcgctggcg
     3181 gagctggcga ttcgcgcagg cgttccggct ggcgtattta acgtggtcac cggttcggcg
     3241 ggcgcggtgg gtaacgaact gaccagcaat ccgctggtgc gcaagctgtc gtttaccggc
     3301 tcgaccgaaa ttggccgcca attaatggaa cagtgcgcga aagacatcaa gaaagtgtcg
     3361 ctggaactgg gcggcaacgc gccgtttatc gtctttgacg atgccgacct cgacaaagcc
     3421 gtggaaggcg cactggcctc gaaattccgc aacgccgggc aaacctgcgt ctgcgccaac
     3481 cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca gcaggcggtg
     3541 agcaaactgc acatcggcga cgggctggag aatggcgtca ccatcgggcc gctgattgat
     3601 gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa aggtgcgcgt
     3661 gtggtttgcg gcggtaaagc ggatgaacgc ggcggcaact tcttccagcc gaccattctg
     3721 gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc ccttgccccg
     3781 ctattccgct ttaaagatga agccgatgtg attgcgcagg ccaatgacac cgagtttggc
     3841 ctggccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtggg cgaggcgctg
     3901 gagtacggca tcgtcggcat taatactggc attatttcca atgaagtggc cccgttcggt
     3961 ggcattaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga agattactta
     4021 gaaatcaaat atatgtgcat cggtctttaa ctggagaacg cgaatgagca gcaataaaga
     4081 gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc accccatttt
     4141 cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt atattgattt
     4201 cgcgggcggc attgcggtgc tcaataccgg gcacctgcat ccgaaagtgg ttgcggcggt
     4261 ggaagcgcag ttgaaaaagc tgtcgcacac ctgcttccag gtgctggctt acgaaccgta
     4321 tctggagctg tgcgagatta tgaatcagaa ggtgccggga gatttcgcca aaaaaacgct
     4381 gctggtaacg accggttctg aagcggtgga gaacgcggtg aaaatcgccc gcgccgccac
     4441 caaacgcagc gggaccatcg cctttagcgg cgcgtatcac gggcgcacgc attacacgct
     4501 ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg ggactgatgc ctgggcacgt
     4561 ttatcgcgcg ctttatcctt gcccgctgca cggcatcagt gaagatgacg ctatcgccag
     4621 cattcaccga atttttaaaa atgacgctgc gccggaagat atcgccgcca tcgtgattga
     4681 gccggttcag ggcgaaggcg gtttctacgc cgcgacgcca gcctttatgc agcgtttacg
     4741 cgcgctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga gcggcgcggg
     4801 gcgtaccggc acgctgtttg cgatggagca gatgggcgtt gcgccggatc ttaccacctt
     4861 tgcgaaatcg atcgcgggcg gcttcccgct ggcgggcgtc accgggcgcg cggaagtgat
     4921 ggatgccgtc gctccaggcg gtctgggcgg cacctatgcc ggtaacccga ttgcctgcgt
     4981 ggcagcgctg gaagtgttaa aggtattcga gcaggaaaat ctgctgcaaa aagccaacga
     5041 cctggggcat aagctgaaag acggattgct ggcgatagcc gaaaaacacc cggagatcgg
     5101 cgatgtacgc gggctgggag cgatgattgc catcgagctg tttgaagatg gcgatcacag
     5161 caaaccggac gccaaactca ccgccgagat cgtggcgcgt gcccgcgata aaggcctgat
     5221 tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccgc tcaccattga
     5281 agacgctcag atccgtcagg gtctggaaat catcagccag tgttttgatg aggcgaagca
     5341 gtagatccgt acccatgccg gaggcgacgc tgcgcgtctt gtccggccta cgcgaatcca
     5401 ggtcggataa agcgtttacg ctgcatccga caatctgtac gtgaacagga ataaatctat
     5461 gtcggccgga taaggcggag ccgctctccg gtaaaaagaa ccaataacaa ttacacgcgt
     5521 gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga gaggattcag
     5581 gatggggcaa tcatcgcaac cacatgagtt aggcggcggg ctgaagtcac gccacgtcac
     5641 catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat
     5701 cgccgaagcg ggcccggcgg tattactggc ctatctgttc gccggattac tggtggttat
     5761 gattatgcgg atgttggcgg aaatggcggt tgccacgccc gataccggtt cgttttccac
     5821 ctatgccgac aaggcaattg gccgctgggc gggttatacc atcggctggc tgtactggtg
     5881 gttttgggta ctggttatcc cgctggaagc caacatcgcc gccatgatcc tgcactcgtg
     5941 ggttccaggc attcccatct ggttattttc cctggtcatt accctcgcct tgactggcag
     6001 taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc tgtgcaaagt
     6061 catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt tttaccctta
     6121 tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc ccaacggctt
     6181 cggcgcagta ttaagcgcga tgttgatcac catgttctcg tttatgggcg cagaaattgt
     6241 caccattgcc gccgcggaat ctgacacgcc ggaaaaacat attgtccgcg ccaccaactc
     6301 ggttatctgg cgtatttcta tcttctattt atgctctatt tttgtcgtgg tggcattaat
     6361 accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc tggagttgct
     6421 caatattccc catgcgaaat taatcatgga ctgcgtgata ttactttccg taaccagttg
     6481 cctgaactcg gcgctgtata ccgcttccag aatgctctac tccttaagcc gtcgcggtga
     6541 tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtatgtgg cggtattact
     6601 ctcgaccggt gcggcatttt taacggtggt ggtgaattat tacgcgcctg cgaaagtgtt
     6661 taaatttctg atcgatagct ccggcgctat cgccctgctg gtttatttag tcattgccgt
     6721 ttcacaattg cggatgcgca aaattctgcg agcagaagga agcgaaattc gcctgcggat
     6781 gtggctttat ccgtggctca cctggctggt catcggtttt ataacctttg tgttggtagt
     6841 gatgctattc cgcccggcgc aacagttaga agtgatttcc accggtttat tagcgatagg
     6901 gataatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt ggcaaaaaac
     6961 acccgttcat aatacgcgct aataatgatc aggagtcaca ccatgaccat tacgtctctg
     7021 gatggctatc gctggctgaa gaacgatatt attcgcggaa actttcaacc ggatgaaaaa
     7081 ttacggatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt gcgggaagcg
     7141 ctttcgcaac tggtagcaga acgactggtc acggtggtga atcaaaaagg gtatcgggtg
     7201 gcgtctatgt cagagcaaga gctgctcgat attttcgacg cccgcgccaa tatggaagcg
     7261 atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga cgttctcgca
     7321 aaagcgcatc tgctcagtaa gctggaggcc tgtgacgcca gtgagaaaat gcttgatgag
     7381 tgggatctgc gtcatcaggc gtttcatact gcaattgtgg cgggctgtgg ttctcactat
     7441 ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt tatctggctg
     7501 cggcgaacag tgctttcggt ggaaatgctg gaagataaac gcgatcagca ccagaccctc
     7561 actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg ccagcattta
     7621 ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact cttcaggaat
     7681 acgcaacact tgccccgggt aaattttatc cgggcttttg agcatcggtt tattcgcttc
     7741 gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg cactcagagt
     7801 gtcgccagac ttaacggtat aaaactggct ggcagcggct ggtgtcgccg ttttcacctg
     7861 atcatcgaca ctggcaatac cggaaatatt ccccaccgca acaaggattt tctccttcgc
     7921 ctcctgactt aagccgtcac ccgtgaccgt cgctttgccg tcggcgattt gaatgttcac
     7981 tttatcggca tcaggtattc cggttttgtt cagatgctcc tgcaccttct tcgcctgatc
     8041 gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat ctttcacaaa
     8101 attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag tgtagcagag
     8161 gaacgcggcg cgttaacgcc gcgggggtga acacaggttt aatcgcgggt ttgcacccag
     8221 aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt aataatgaac
     8281 gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat ggtgatgacg
     8341 attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt aaaaagagat
     8401 tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta cgcttttact
     8461 ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt tacttaattt
     8521 agaaaatact taaatatcta tgactgaact cgcgcaatta caggctagtg ccgaacaggc
     8581 agcggcctta ttgaaagcaa tgagccaccc taaacggttg ctgattctgt gcatgcttag
     8641 cggttccccc ggcaccagtg cgggagagct gacgcgcatt accggactga gtgcctctgc
     8701 aacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc aacgggatgc
     8761 ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg ccaccctgaa
     8821 aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc cgcatgatgc
     8881 acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgacgctg atgaatatct
     8941 tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac agtcaggtct
     9001 tccggctaaa ttacgtcgcg agcaaataat attccactgc caggctggta aacgcaccag
     9061 taataacgcc gataaattag cagcgattgc cgcccccgca gaaatctttt tactcgaaga
     9121 tgggattgat ggctggaaaa aagcaggatt accagtagcg gtaaataaat ctcaaccctt
     9181 gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg gcgttatact
     9241 aggttatacc gtaaatagcg gtttcttctt attaagcggc tttgttggtg ccgggttact
     9301 gtttgcagga attagcggtt tttgtgggat ggcaaggttg ttagataaga tgccgtggaa
     9361 ccaacgagct tgagaagcga cgccggacgc gccatagcag caacatccgg cctcagtaat
     9421 tagatcagaa aatcgtcgag agatttacct tctgccagcg cctgagcgat tggcttcggt
     9481 gtacggccct gaccggtcca ggttttagtt tcaccgttaa catcgatgaa tttatatttc
     9541 gccggacgcg gctggcgttt tttaccagcg cgtggtgccg cagcagagct attacccaat
     9601 aactcttccg ggttaattcc gtcagctttc atcagctcca gccaggtgct aattttttcc
     9661 tggcgctctg ccagttcacg ctgctgttgt tcttcttctt cacgtctttc tttagtgaca
     9721 accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg agccatcgca
     9781 cggagggtgc gaatgttatt taaactttgt aacattacgg acataataaa gaaaaccttt
     9841 taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta ttatatttcc
     9901 acaaaattta atctatttac agtcctgcac gagattattt cagctgttat tttctgaaac
     9961 cctcagaata atttcaagat tatatattag ccattttgat gagtaattta ggttattggt
    10021 gaacagagcg gcgctctctg ctcgctaatt ccgcaagccc gacaaaacca aagtgaataa
    10081 aaatgcaact caacagaata aaagaaaaca tctatcactg gtgctttctg ctgtcaatgt
    10141 acattatctg gcataaaaaa tgctgaaaat gacccgatca cta
//
LOCUS       DAZKJR010000173        10047 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKJR010000173 DAZKJR010000000
VERSION     DAZKJR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.174"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            220..1377
                     /gene="acrE"
                     /locus_tag="ACHOWS_004504"
     CDS             220..1377
                     /gene="acrE"
                     /locus_tag="ACHOWS_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFQ0049985.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1389..4493
                     /gene="acrF"
                     /locus_tag="ACHOWS_004505"
     CDS             1389..4493
                     /gene="acrF"
                     /locus_tag="ACHOWS_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFQ0049986.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4746..4967
                     /locus_tag="ACHOWS_004506"
     CDS             4746..4967
                     /locus_tag="ACHOWS_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049987.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5398..6423
                     /locus_tag="ACHOWS_004507"
     CDS             5398..6423
                     /locus_tag="ACHOWS_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0049988.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6491..7672
                     /gene="yhdX"
                     /locus_tag="ACHOWS_004508"
                     /pseudo
     CDS             6491..7672
                     /gene="yhdX"
                     /locus_tag="ACHOWS_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            7682..8785
                     /gene="yhdY"
                     /locus_tag="ACHOWS_004509"
     CDS             7682..8785
                     /gene="yhdY"
                     /locus_tag="ACHOWS_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0049989.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKNQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8793..9551
                     /gene="yhdZ"
                     /locus_tag="ACHOWS_004510"
     CDS             8793..9551
                     /gene="yhdZ"
                     /locus_tag="ACHOWS_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0049990.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(9614..9729)
                     /gene="rrf"
                     /locus_tag="ACHOWS_004511"
     rRNA            complement(9614..9729)
                     /gene="rrf"
                     /locus_tag="ACHOWS_004511"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9769..9844)
                     /locus_tag="ACHOWS_004512"
     tRNA            complement(9769..9844)
                     /locus_tag="ACHOWS_004512"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9809..9811),aa:Thr,seq:ggt)
     gene            complement(9859..9974)
                     /gene="rrf"
                     /locus_tag="ACHOWS_004513"
     rRNA            complement(9859..9974)
                     /gene="rrf"
                     /locus_tag="ACHOWS_004513"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2382 a         2409 c         2588 g         2668 t
ORIGIN      
        1 cgaggatttg attctacctc tttaaacccg cgtcaaaata aaacagtaga atattaatct
       61 ttttttgtgt ttatgtgcct tgagacgcca gtattcataa ctattcctta catcgacgaa
      121 tgataatttg taggatagcg aactgtattt ttctttctgc gagttaacgc gttgcctttt
      181 tgggtaaata acgcgctttt ggttttttga ggaatagtaa tgacgaaaca tgccaggttt
      241 ttcctcctgc cctcctttat tctgatctcc gcggctttaa tcgccggttg taacgataag
      301 ggagaagaga aagctcacgt cggtgaaccg caggttaccg ttcatattgt gaaaacggcc
      361 ccgttagaag ttaagactga attaccaggc cgcaccaatg cttaccgtat agccgaagtt
      421 cgcccacagg ttagcgggat cgtactgaat cgcaatttca ctgaaggcag cgatgtgcaa
      481 gcaggccagt ccctgtacca gatcgatccc gcgacctatc aggcaagtta tgacagcgcg
      541 aaaggcgaac tagcgaaaag tgaagccgcc gccgccatcg cgcatttgac ggtaaaacgt
      601 tacgttccgc tcgtgggtac gaaatacatc agtcagcagg agtacgacca ggccattgct
      661 gatgctcgcc aggccgatgc cgccgtgatt gccgcaaaag ctacagttga aagcgctcgc
      721 atcaatctcg cttataccaa agtgactgcg cctattagcg gacgtatcgg caaatcgact
      781 gtgaccgaag gcgctcttgt cactaatggg caaacgactg aactggcgac tgtccagcag
      841 ctcgatccta tctacgttga tgtgacccaa tccagcaacg attttatgag gctgaagcaa
      901 tccgtagagc aaggaaattt gcataaggaa aacgccacca gcaacgtaga gttggtcatg
      961 gagaacggtc aaacctatcc cctgaaaggt acgctgcaat tctccgatgt gaccgttgat
     1021 gaaagcaccg gctccataac cctacgtgct gtcttcccta acccgcaaca tacgcttttg
     1081 ccgggtatgt ttgtgcgcgc acggattgat gaaggcgtcc aacctgacgc cattcttatc
     1141 ccgcaacaag gcgttagccg cacaccacgt ggtgatgcaa ccgtgctgat tgttaacaat
     1201 aaaagtcagg ttgaagcgcg ccctgtcgtt gccagccagg cgattggcga taaatggttg
     1261 attagcgaag ggctgaaatc tggcgatcaa gtcattgtca gcggcctaca aaaagcgcgt
     1321 ccgggagagc aggttaaagc cactaccgat acccccgcag atactgcatc gaagtaaggc
     1381 aatctgacat ggcaaacttt tttattcgac gaccgatatt tgcatgggtg ctggccatta
     1441 ttctgatgat ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag tatccaacca
     1501 ttgcaccgcc tgcggtttct gtttcagcaa actatccggg cgctgatgcg cagaccgtgc
     1561 aggatacggt gacgcaggtt atcgaacaga atatgaacgg tatcgataac ctgatgtata
     1621 tgtcctccac cagcgattcc gccggtagcg tgacaattac ccttaccttc cagtccggga
     1681 ccgatcctga tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc acgccgttgc
     1741 tgccgcagga agtacagcag caggggatca gtgttgaaaa gtccagtagc agctatttga
     1801 tggtggcggg ctttgtctct gataacccag gcaccacaca ggacgatatc tcggactatg
     1861 tggcctctaa cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac gtacagcttt
     1921 tcggcgcaca gtatgcgatg cgtatctggc tggacgccga tctgctaaac aaatataaac
     1981 tgacaccggt tgatgtgatt aaccagttga aggtacagaa cgatcagatc gctgccggac
     2041 agttgggcgg aacgccagcg ttaccagggc aacagttgaa cgcctcgatt attgctcaga
     2101 cgcggttaaa aaatccggaa gaattcggca aagtgaccct gcgcgtaaac agtgacggct
     2161 cggtggtacg cctgaaagat gtcgcacggg ttgaacttgg cggtgaaaac tacaacgtta
     2221 tcgctcgtat caacggaaaa ccggcggcgg gcctggggat taagctggca accggcgcaa
     2281 atgctctcga taccgcgaaa gccattaagg caaaactggc ggaattacag ccattcttcc
     2341 cgcagggaat gaaggttctc tacccttatg acaccacacc attcgtccag ctttctattc
     2401 acgaagtggt aaaaacgctg ttcgaagcca ttatgctggt gttcctggtg atgtatctgt
     2461 tcttgcagaa tatgcgagca acgctgatcc ccaccattgc ggtacccgtg gtgttgttag
     2521 ggacgtttgc catcctcgcc gcttttggtt actccatcaa cacactaacg atgttcggga
     2581 tggtgcttgc catcgggctg ctcgtcgatg atgcgatagt ggtggtggag aacgtcgagc
     2641 gcgtgatgat ggaggataag ctcccgccaa aagaagcgac ggaaaaatca atgtcgcaaa
     2701 tccagggcgc actggtgggt atcgcaatgg tgctgtcagc ggtatttatt ccgatggcat
     2761 tcttcggcgg ttctactggg gcaatttatc gtcagttctc tatcaccatc gtttcggcaa
     2821 tggcgctttc tgttctggtg gcattgattc ttacccctgc gttatgtgca acgctgctta
     2881 aacccgtctc tgctgagcat cacgaaaata agggcggttt cttcggctgg tttaatacca
     2941 ccttcgatca tagcgttaac cactacacca acagcgtcgg caaaatcctc ggttccacag
     3001 gacgatattt actgatctat gcactaattg ttgcaggaat ggtggtgttg tttttacgtc
     3061 ttccgtcttc cttcttacct gaagaggatc agggtgtctt tctgactatg attcagttac
     3121 ccgctggcgc gacgcaagag cggacgcaaa aagtactgga tcaagtgacg gattactatc
     3181 tgaagaacga gaaagcgaac gttgaaagtg tctttacagt taacggcttt agcttcagcg
     3241 gccaggcaca aaacgccggt atggctttcg tcagtctgaa accgtgggaa gagcgtagtg
     3301 gtgacgaaaa cagtgcggaa gcggtaatcc atcgtgccaa aatggaattg ggcaagatcc
     3361 gcgacggttt tgtcattcca ttcaatatgc cagccattgt tgaactgggc acggcaacgg
     3421 gtttcgactt tgagttaatt gatcaggctg ggctgggtca tgatgccctg acccaggccc
     3481 gtaaccagtt gcttggcatg gcggcgcaac atcctgccag cttagtcagc gtgcgtccta
     3541 atggtctgga agacaccgcg cagtttaaac tggaagttga ccaagaaaag gcgcaggcat
     3601 taggtgtttc actttctgac atcaatcaga ccatttcaac agcgttgggt gggacttacg
     3661 ttaacgactt cattgaccgt ggtcgcgtaa aaaaggtgta tgttcaggcg gatgccaaat
     3721 ttcgtatgct gccagaagat gtcgataaac tttatgtccg tagcgccaat ggcgaaatgg
     3781 tgccattctc tgcctttacc actgcacatt gggtgtatgg ttctccgcga ctggagcgct
     3841 acaacggtct gccgtcaatg gagattcagg gggaagccgc gccaggaacc agttccggcg
     3901 atgccatggc gttgatggaa aaccttgcat cacaattacc agctggtatt ggttatgact
     3961 ggacgggtat gtcatatcag gaacgcttat cgggaaacca ggctcccgct ctggtagcaa
     4021 tttcctttgt ggttgttttc ctgtgccttg ctgcactcta tgaaagctgg tcaattcctg
     4081 tctcggttat gttggtcgtg ccgttaggga ttgtcggcgt gctactggcg gcgacactct
     4141 ttaatcaaaa aaatgacgtc tactttatgg tgggcttgct aacgacaatt ggcttgtcgg
     4201 ctaaaaacgc tatattgatt gttgagttcg ctaaagatct catggagaaa gagggtaaag
     4261 gtgttgttga agcgacacta atggcagtac gtatgcgtct gcgtcctatc ctgatgacct
     4321 ctctcgcctt tattctcggc gtattaccgc tagctatcag taacggtgcc ggcagtggcg
     4381 cgcagaacgc agtgggtatc ggggtaatgg gcgggatggt ttctgcaacg ttgctggcaa
     4441 tcttcttcgt gccggtgttc tttgtggtga tccgccgttg ctttaaagga taaatcagaa
     4501 acataaaggc actttcgggt gcctttatta tttccagtga aacccataaa aattaataag
     4561 atattcctct actcactctt taaaaacttt ctatagttcc cgctcccttc actattttta
     4621 caattcacat aatttgtcat tattcattcc gcaagattta taacctccag tgataaaatg
     4681 gcattcagct cgttaataag agagttaact tattaagcgt tagcgtttat tactgaggta
     4741 acaccatgaa aagattaatt cctgtggcat tgctcaccgc attgctggcg ggctgtgcgc
     4801 acgattcgcc ctgcgttccg gtttatgacg atcagggtcg tctggttcac accaatacct
     4861 gtatgaaagg cacgactcag gataactggg aaacggctgg ggctattgcc ggcggggcag
     4921 cagctgttgc tggcctgacg atggggatca tcgctctgtc gaaataacta aacagggcgc
     4981 ggagctccgc gctcttttta acgatacagt atctttccat aagataaaat attgctatat
     5041 tctgctgggt aattccctgc tgatttagca tgttatatca cgttatttca ctgctttcga
     5101 aataacgcat caatttaact ttcacacatc ccaccagaaa tatactcacg ctaacaatca
     5161 ttattctgct tagtaaaaaa cgcgtaaata ttttgcgcca aaatgtggcg catgtttcat
     5221 tttcgcacca ttgcggggcg cagtttttat tatttcctgt ctacactcag cttattgcga
     5281 cgcgaaatcg tgcgcaaaag ctggcactac ttttgcttat aagaggatgg ccacagacag
     5341 gtaaaagacg tttcccaaac gtcctataac gataattttc gcctcacagg aagcattatg
     5401 aaaaagatga tgatagccac actggctgcc gccagcgtgc tgcttgccgt tgcaaatcag
     5461 gcgcatgctg gcgcgacgct tgatgccgtt cagaaaaaag gttttgtgca atgcgggatc
     5521 agtgatggat tacctgggtt ctcttatgcc gatgctgacg gtaagttttc aggtattgat
     5581 gtagatgttt gtcgtggtgt tgccgctgct gtattcggtg acgatacgaa agtgaaatat
     5641 accccgctca ctgcaaaaga acgcttcacc gctttacagt caggggaggt ggatttgctc
     5701 tcccgtaata cgacctggac ttcatctcgc gatgccggga tgggaatggc atttactggc
     5761 gtcacttatt acgacggcat tggcttcctg acgcacgata aagcggggct aaaaagcgcg
     5821 aaagaactgg atggcgctac cgtctgtatt caggcgggta ctgataccga actcaacgtt
     5881 gccgattact ttaaggcaaa caatatgaag tacacaccag tgactttcga tcgctctgac
     5941 gaatcagcga aggcactgga atctggtcgc tgcgatacgc tggcctcgga tcaatcacaa
     6001 ctgtatgccc tgcgcatcaa attaagcaac ccggctgaat ggattgtctt accggaagtt
     6061 atctctaaag aaccacttgg gccggtagtt cgtcgtggcg atgatgaatg gttctcgatt
     6121 gtacgctgga cgcttttcgc catgctgaat gctgaagaga tgggcatcaa ttcccagaac
     6181 gtcgatgaaa aagcggctaa tccagcaaca cctgatatgg cacatctgct gggtaaagaa
     6241 ggcgattacg gcaaggatct gaagctggat aataaatggg cctttaacat catcaaacag
     6301 gtgggtaact actcggaaat tttcgagcgt aacgtaggtt cagaaagccc gctgaaaatt
     6361 aaacgtgggc aaaataatct ctggaataac ggcggtattc agtacgcgcc gcccgtgcgt
     6421 taagtcggtg tgaggtaacg agcgtcgcta cggcggcgct ctcttcagcg ttatgctttc
     6481 cgaggtttct atgtctcatc gccgctcaac cgttaaaggc tcactctcct ttgccaaccc
     6541 tacggttcgc gcctggttat tccagatctt tgccgttgtt gctgttgtcg gaattgttgg
     6601 ttggctattt cacaacactg tgacgaatct cagtaatcgt ggcattactt caggttttgc
     6661 ctttctggat cgcggcgctg gcttcggtat tgtccagcat ttgatcgatt accagcaggg
     6721 tgatacctac ggacgcgttt tcattgttgg cttactcaat acgctactgg tttctgcatt
     6781 gtgtattgta ttcgcttctg ttctgggctt ctttatcggt ctggcgcgac tttcggataa
     6841 ctggctgcta cgaaagcttt ccacaattta tattgagatc ttccgtaata ttccgccgct
     6901 gctgcaaatc ttcttctgat actttgccgt gttgcgcaat ttgcccggac cacgccaggc
     6961 agtgagtgcg tttgatctgg cctttttgag caatcgtggg ctttatattc catctccgca
     7021 gctgggagac ggatttattg cgtttatcgt gactgttgtt attgctatag ccatttctgt
     7081 tggactattc cgttttaata aaacgtacca gataaagacc ggacaattgc gccgaacctg
     7141 gccggtcgcc gttatgttga tcataggttt gcctttactg gcgcaatggc tttttggcgc
     7201 agcactacac tgggatgttc cagccctacg aggctttaac ttccgtggcg ggatggtttt
     7261 aattcctgaa ctggcagcct taacgctggc gctttcggtt tatacctctg catttatcgc
     7321 cgagattatc cgcgctggga tccaggcagt gccttatggt caacatgaag cggctcggtc
     7381 actgggatta cccaatccgg ttacgctacg ccaggtcatt attccccagg cactgcgagt
     7441 gattattccg ccgttaacca gccagtatct caacatcgtc aaaaactcct ctcttgccgc
     7501 cgctattggc tatccggata tggtttcgct gtttgctggc accgtgctga accagacggg
     7561 gcaagccatc gagacgatag ccatgaccat gtcggtctat ctgattatca gcctgactat
     7621 ctcgctgctg atgaatatct ataaccgccg catcgcgatc gttgaacgct aaggagtcat
     7681 gatgacaaaa gtattgctgt ctcatccccc gcgcccggcg agccataact caagccgcgc
     7741 gatggtgtgg gtgcgaaaaa atctgttctc cagctggagc aatagcctgc tgactattgg
     7801 ctgcatatgg ttgatgtggg aactgattcc accgttgctg aactgggcat ttttgcaggc
     7861 taactgggtt ggctcaacgc gtaccgactg cacaaaagcc ggtgcctgtt gggtcttcat
     7921 ccacgaacga tttggtcagt ttatgtatgg gctttaccca cacgaccaac gctggcgaat
     7981 taacctcgca ttactgattg ggcttgtgtc gatcgcacca atgttctgga aaatactccc
     8041 gcatcgcggt cgctatattg cggtatgggc ggtgatttac ccactgattg tctggtggct
     8101 gatgtatggc gggtttcttg gtcttgagcg ggttgaaacc cggcaatggg gcgggctgac
     8161 gctaacttta attattgcat cagttgggat tgcgggggcg ctgccgtggg ggatcttact
     8221 ggcgttaggt cgccgctccc atatgccgat tgtgcgtatc ttatcggtca tttttatcga
     8281 gttctggcgc ggtgtaccgc tgataaccgt tctgtttatg tcttcggtca tgctgccgtt
     8341 gtttatggca gaaggcacca gtatcgacaa attgatccgc gcgctggttg gcgtgatcct
     8401 gtttcagtca gcatatgttg cggaagtcgt gcgaggcgga ttacaggcgc tgcctaaagg
     8461 gcaatatgaa gcggcagagt cgctggcgtt gggttactgg aaaaaccagg ggctggttat
     8521 tctgccacag gcgttgaagc tggtgattcc tgggctggta aataccatca tcgcactctt
     8581 caaagatacc agcctggtga tcatcatcgg gttgttcgat cttttcagta gcgttcagca
     8641 ggcaaccgtt gatcccgcct ggttgggtat gtcgacggaa gggtatgttt tcgccgcact
     8701 gatctactgg atcttctgtt tcagcatgtc acgttatagc caacatctgg aaaaacgttt
     8761 taacaccggg cgtacaccgc attgaggaca ctatgagcca aattttactg caacctgcta
     8821 acgcgatgat tacgctggaa aacgtcaata aatggtatgg gcaattccat gttttgaaaa
     8881 atataaattt aaccgtgcaa ccgggagaac ggatcgttct gtgtggccct tctggttccg
     8941 gtaaatcgac aaccattcgt tgtattaatc atctggaaga acaccaacag ggacggatcg
     9001 tggtagatgg catcgagctt aacgaagata ttcgcaatat tgagcgcgtc aggcaggaag
     9061 tgggaatggt ctttcagcat ttcaatctct tccctcatct gaccgttcta cagaactgta
     9121 ccctggcacc gatttgggta cgcaagatgc caaagaaaga ggcagaagct ctggcgatgc
     9181 attaccttga gcgggtgaga attgccgaac atgcgcataa gtttcccgga cagatttcag
     9241 gtggtcagca gcaacgcgtt gccattgcgc gttcgctgtg tatgaagccg aaaattatgt
     9301 tatttgatga gccaacgtcg gcgctcgatc ctgagatggt gaaagaggtg ctggatacga
     9361 tgattgggct ggcgcagtcg ggtatgacaa tgctgtgtgt aacacatgag atggggtttg
     9421 cacgaaccgt cgctgacagg gtgattttta tggatcgtgg ggagatagtg gaacaagctg
     9481 caccggatga gttttttgcg catcctaaat cagagcgtac gagggcattt ttatcgcagg
     9541 tgatccatta attgaatgtt agttcgaaaa gcaaaaaggc catcctttcg gatggccttt
     9601 cgcttgattt gatgtctggc agttccctac tctcgcatgg ggagacccca cactaccatc
     9661 ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact
     9721 gccgccagac aaattctttt ctaatctgcc gaactttaac ctaaaaagtg gtgctgatac
     9781 ccagagtcga actggggacc tcacccttac caagggtgcg ctctaccaac tgagccatat
     9841 cagcacgcta aatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta
     9901 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
     9961 ctaaggccgc caggcaaatt ctgttttatc aacacgtctc gttatgacct gtcgatttaa
    10021 tctgtatcag gctgaaaatc ttctctc
//
LOCUS       DAZKJR010000174         9765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKJR010000174 DAZKJR010000000
VERSION     DAZKJR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.175"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..173)
                     /locus_tag="ACHOWS_004514"
     CDS             complement(<1..173)
                     /locus_tag="ACHOWS_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0049991.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQ"
     gene            complement(223..525)
                     /locus_tag="ACHOWS_004515"
                     /pseudo
     CDS             complement(223..525)
                     /locus_tag="ACHOWS_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(552..974)
                     /locus_tag="ACHOWS_004516"
     CDS             complement(552..974)
                     /locus_tag="ACHOWS_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFQ0049992.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKAS"
     gene            complement(991..1740)
                     /locus_tag="ACHOWS_004517"
     CDS             complement(991..1740)
                     /locus_tag="ACHOWS_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0049993.1"
                     /translation="MTLPNPLAPVKGSGTTLWVYTGNGDAWANPLSDDDWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            complement(1748..2146)
                     /gene="gpU"
                     /locus_tag="ACHOWS_004518"
     CDS             complement(1748..2146)
                     /gene="gpU"
                     /locus_tag="ACHOWS_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFQ0049994.1"
                     /translation="MKHREIRAAILSALKGNIADRVTWFDGRPVFIDEQELPAVAVYL
                     TDASATEEFVDEGTWEAVLHIEVFLRAKETDSVLDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEYMMWGSADLQWNITYQM"
     gene            complement(2156..2782)
                     /locus_tag="ACHOWS_004519"
     CDS             complement(2156..2782)
                     /locus_tag="ACHOWS_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0049995.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMTAMTHQLRLVLKRKS"
     gene            complement(2785..3066)
                     /locus_tag="ACHOWS_004520"
     CDS             complement(2785..3066)
                     /locus_tag="ACHOWS_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFQ0049996.1"
                     /translation="MSDLFTRMCCRMDGATVRVMGKQAEINGVVYDVMPEEESADMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(3059..3385)
                     /locus_tag="ACHOWS_004521"
     CDS             complement(3059..3385)
                     /locus_tag="ACHOWS_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFQ0049997.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     DAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(3473..5452)
                     /locus_tag="ACHOWS_004522"
     CDS             complement(3473..5452)
                     /locus_tag="ACHOWS_004522"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFQ0049998.1"
                     /translation="MKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFVSELNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGERALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(5442..6944)
                     /locus_tag="ACHOWS_004523"
     CDS             complement(5442..6944)
                     /locus_tag="ACHOWS_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0049999.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(6944..7156)
                     /locus_tag="ACHOWS_004524"
     CDS             complement(6944..7156)
                     /locus_tag="ACHOWS_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050000.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(7153..9276)
                     /locus_tag="ACHOWS_004525"
     CDS             complement(7153..9276)
                     /locus_tag="ACHOWS_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFQ0050001.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWTTSGGWL"
     gene            complement(9273..9749)
                     /locus_tag="ACHOWS_004526"
     CDS             complement(9273..9749)
                     /locus_tag="ACHOWS_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFQ0050002.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGRLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2077 a         3073 c         2344 g         2271 t
ORIGIN      
        1 ctctgctcag cagcatcagc ctgttttctg aaagactgac tgactttccc ggacatcaaa
       61 tcagcaagcg cactggtttc acccagctct ttttttaccc gcgctgcctc ttcagaaaaa
      121 cgggcagaat ccagtgtcag cacggctgtc agatcggcaa aattacctgc catcgtggtc
      181 acctcctgaa atgtcctctg atgccatcaa taactgtcac aacctccttc cctcctcccc
      241 gaaacggact ccaccggcga ggcccgccgc cttctgcatc agtacatcat tttcgtccgg
      301 cgtctccgcc tgcccttcct ctgcctccgg agcgaacagg ctgaaatccg ccggatgcat
      361 atccggatcg caaaaaaaca ggctgagtac ggcgtacgtc agcccggaaa aatgcatatc
      421 cagctgggta tcctgaaaat aatgcgtgca gtaaaaacgt cgccagtcgg catattcggt
      481 ggatgtcatc ccggcaagca tggcgcgcca gtcggctctc cccatctcac gcgccagttt
      541 caggacaaag ttcagctcgc cttcgaatgc ttttttgatg ttaccggctc agtcgcttct
      601 gctttcccgg ttggttcagg atcggcatcg tgccggttat ccagcatacc tgaaagataa
      661 agcacccggt tcgttgcctg attcagtgca tcagcaggcc atcccagcat cacttcacgg
      721 cggatctgct gcatctctgt ctccggagag gccagagtgc ctttgaggga atgggaatgc
      781 catagcgaca tcgccacaag cagggatgcc gtttccagat atcgctggtt aatgtgcacg
      841 gcatcatgct ccgttgtctc ctgttgtgct gcgtctgaaa caaacttcat ataatcaaac
      901 cgctgcagcg cggacagctc cgacagcgtg acggacacac cgttatattc aaattgttct
      961 gttttcagga acatgcttta tctcctcccc tcagcccgca gcgccatccg tgacggtgat
     1021 ctccgccacc gccgcaaact caccgttgcc ggtgacaaca gggatctgcg ctttacctgc
     1081 cgcaacacct ttcacggtga tcgtgttccc cttcacggta atggtggcga aattctgatt
     1141 cgctgacgtt gcgcggaatg ttttatccgt tgcgccttcc ggctgaacgg ccacggtcag
     1201 ggtgatattc tgaccttttg ccacattccc cgttagtggc gtcacggtaa taccggtgac
     1261 cggtgtgatt tctccccgat cctcagccag cgacggacga ccaatattgg tgattttcac
     1321 agtgcgggtg atcacttctt tggcggtcac ggatttacca atggcgctca cccagccacg
     1381 gaacacatcc accgtgccgt tcgggaagcg gattttgtag gcccgggtct cgctgctgtc
     1441 aaaccaggca atcaaatcac gctgcccttt ctcgccgggc ttccaggcca gcgtaaaact
     1501 ggtgtcaccg gcggatttct gcccctgccc ggtggatacc cagtcagcat cctcatcatc
     1561 cagatagtta tcgtcgtagg attccgccgt catctcgccg ggggtcagat cctttatttt
     1621 tgccaggcgc gtccagtcat catctgacag cgggtttgcc caggcatcgc catttccggt
     1681 ataaacccag agcgtggtgc cggaaccttt taccggcgca aggggattag ggagcgtcat
     1741 aacgtcctca catctggtaa gtaatattcc actggagatc agccgatccc cacatcatat
     1801 attcgtcatc ccggcgatag tcatacccct gaagattcat cttcagcagt aatgcactga
     1861 gcccgggaac cgcctccagc gccggaagta ttttttcttc catccacata tccagcacgg
     1921 agtccgtctc tttcgccctg agaaacacct ctatatgaag cacggcctcc caggtcccct
     1981 cgtcaacaaa ctcctccgtc gcagaagcat ctgtcagata aaccgccacc gcaggcagtt
     2041 cctgttcatc aataaatacc ggacgaccat caaaccaggt cacccggtca gcgatatttc
     2101 ccttcagggc agacaatatt gccgcccgaa tttctctgtg tttcacattc cctccttacg
     2161 attttcgttt cagcaccagg cgtaactgat gcgtcatggc cgtcatcatc tgcgccggta
     2221 atttttcccg gtacatccgg tcccgttcac gttcaaaggt ttctgccagc ggtccggcag
     2281 tcggaatctt caccacttcg atcggcagac ggtggcgttt cggcctccct ttgctgtccg
     2341 cgccggtgga cgatgatgcc cacggcatac gctgcatcac atgccagcgt ccgttagcca
     2401 gccgggtgat aaaggcgtcc gggatccgtc ttttccccac aatcagcaca ctgccgcccc
     2461 ctttcagggc cgcacgctgt cctttctttc tccgttttct gcgggaaagt cgaacgcggg
     2521 cctcccccag tttgatggcg ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct
     2581 tatccggtcg tgccggactt aaccggatgc gggcacggat aagacgacgg ggaacggcca
     2641 gctccctggc aactgaagag gccgttttcg caataatggc ccccgccacg cggttcagtg
     2701 tcgtggcaga ggcccgggga acggcacggc gatcaattgc atccagattt ttcatggcct
     2761 gcgccagacc ttttattgcc atactcattc ctgttcgaca aaaatccggg gtttaccgtt
     2821 gtacgtgtca taacgggtca ccgtcagtgt gcgccccgca aacacaacaa catcatgacg
     2881 ggccggacgg taccgggctg aaaacaccac cagtgacaac tggctgcccg aaagcgcccc
     2941 catatccgcg gactcttcct ccggcatcac gtcgtacacg acgccgttaa tctccgcctg
     3001 tttgcccatc acacgaacgg tcgccccgtc catccggcaa cacattcgcg taaacagatc
     3061 agacattgat ttttaccgcc acagtggcgc tgtttgcagg ggcattttcc caggctaccc
     3121 ccgcagccac cgcaccgtct gcagccagct gcacaacccc gtccttcaga taaaccaccg
     3181 cgccggactg aatatcgtca gcagactgtt tgggcagcag gaacacgcct tcggcaaaac
     3241 cgtcaccggc atcaccggca ggaatatcgg taatggccac tgccaccata ctgccgacca
     3301 ccaccgcagc accgctcagg atggtctgat ctccggcatt caccagttca atggtggtgc
     3361 cgtcctgtac aaaatttttc gccataatgc tgtttctccg gacagcccct gtggggctgt
     3421 ttttcaggca taaaaaaagc cctttcgggc agtgattgtg ataacgcggt tatcaggcca
     3481 ccgacgaacg caccagaccg cgccagtcaa gtggtgccac accggcatca atacgaattt
     3541 ttgtggcaat gccatcagtg gtgaaacctt cctgctgatc aatgtatggc gtgtccacac
     3601 catccagcca ggccacttca atggtgtcag tgccctgtgc cgccgccaga taccaggttt
     3661 tcggatctgc cgcatcaaga cgcgcttccg caatcacctc agcaaagttc tggatggggt
     3721 taatgacacc ggcgtttgca tccgcccctt tcacactggc cgatttgatg gtctggttcg
     3781 ccaccgtctc cagtgccacc ggtaccagca taaaggccgg acggatattc agggcgcgct
     3841 cgccttcttt ctgcaggcgc atcatctgac gggccgcatc cagtccggaa acggagatcc
     3901 ccccggtggc aatatttttg tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg
     3961 ggttatccgt cagcaccttg taaaccaggt caccaatcgt tgccttcgcc gcacgcccca
     4021 tcttcatcgg cacgtccacc agcatattca gatcatcatt gataatggcc tggcgggtga
     4081 tggagaaaat ctccccgtag gtggccagag caatggtctc cttgcgatct gaggtggtga
     4141 tgtatttata ctccgccccc tcacgaacct gacgcagaga accaaaaccg cccatcccca
     4201 cgcgatacgc tgttttgaag tctgacaggc gtcccttacg ggtccacttc tggaaggttt
     4261 cttctgattc ctcccagccc tggatcagcc ccttgttcga cacatccagc aggatattgc
     4321 caaaatcaga ggtgctgtgc gtcagcgcca gcccgaccat ctgcatgggg ttataactgg
     4381 ccaccccaat accgcgctcc gtcagtgaca tacgagccca ttcacgcagg gtcatcccgt
     4441 tataggcgtt atccttctcg acattttcaa atccggcacg ggccagcatc gcctggcgga
     4501 tcccgtctcc cacaaaattg ccgtttccgg cataaatatg agccggtgta tttttgttgg
     4561 tcggcgagga ctccttgccc atttcattca gcagacgttc acgggccatt tccagcgaac
     4621 agtcaggatc agccacgcac tgtgcctgaa gcgtctgata gcgaccgccg aacatggcaa
     4681 acagatcgtt aatgcctgac atgcgtgctt tctgctcagc cataacgcgg gcgcgaatgg
     4741 tcgcctcatc agacactgcc ggtaccggtg atggttctgt taccgccggt gcagggattg
     4801 tcactgtggt atcacgcggg gcactgttgc gtggtggagt aatcatgttt cggatggatt
     4861 ccggcatctt tttaaattcc tctgtacgtt ttgactgaat acatgccatt gcctcaacag
     4921 cgggtgtcac ctggtcagca aatccgtgtg ccagacattc ggcaccggac atccaggttt
     4981 catccgccag catggcggca atttcatcgg tggtttttcc ggttttctgc gcataggctg
     5041 gcaacagtac cgattcgact ttatccagca aatcagcata actgcgcata tcctcagcat
     5101 ccccgccact gaatccccat ggcttatgga tcatcatgaa ggcattttcc ggcataatga
     5161 ccgtatcacc ggccatcgca atcacagatg ccatcgaggc ggcaacgcca tccacataca
     5221 cggtaatggt cgccccctga tttttcaggg cattaaaaat ggcgatgcct tcaaagacat
     5281 cgccacccgg tgaattgata tggagattaa tgtgggtgat atcacccagt gcattcagtt
     5341 cgctgacaaa ctgcttcgcg gtaactcccc agaaaccaat ctcgtcataa atataaatat
     5401 ccgcgtcacc cggcccccca gcctgcatcc tgaaccagga tttattcttc atgctggctt
     5461 tcggtgtcac gctgatactg tcgttcagtt ccggcactgt tgcctccttt gtcgttgacg
     5521 gggtcagtat caaagaccag ccccagtctg ctgttttcat caatttcagc cttgcggcga
     5581 cgtttgacct catccggatt gcgcccgccg gcacgcaccc agtcagattc tgtcgctgca
     5641 ccaccccgga tctgaattct ccaggcttca gcttccttaa ccgggtcgat ccacggcatc
     5701 accggaccgg aataggtcgc gttatatagc gttttcatct ccacatccgc cggaattttc
     5761 agcagacctg ccgcaaccac catattcagc catgtccggt acaccgggcg ggttaccgcg
     5821 ccaataaaac agtcctgcag gatcaggtaa ccatccgtgg actcgaccag ctcctgccgc
     5881 tgggcactgt aagttccgtt atagttacgc gccgcactgg aaaaactcag acgactgcca
     5941 gctgccactg cacgcaactg gccgttgcgg aaagtttcaa ggttgggatt gggacggtca
     6001 gatttgacca tgccgatatc ctcgcccttg cgcaaatcgt cataaataat acccggggtg
     6061 atatggactt cccgctcggt ttctttgatc cccggatctt catagtcctg tccgtcacct
     6121 ttacggatat acagtcccag cgccgcagca atacgcgccg ctgtcagttc cgcatcctca
     6181 tactctttaa gggcactgat ccgcatcagc acccccgata acatggatga gcctcgcgtc
     6241 tgatgcagac gacgagtgaa cttcaggtgg atcatttttc cggcagcgat ttctttcgta
     6301 tcactctgcc ggccgctgac cggataattt ttataaacca gatatttttt cggtcttccc
     6361 cactcatcaa gaaaaacccc ctgattcagc ccggcggatt catcagtgcg catgggaaca
     6421 aaatccggct ccatcgcctc aagccagaat ggcactcccg ccgtccgttc cagaccgttt
     6481 cccgcaccac tgaccatctg cgcaaacact tcaccatccc gcagccaggt ccgcagcagt
     6541 aaacgttcaa gcacgggacg ggtatactgc cctgtcacat ccggactgac cgaccattca
     6601 gcccacaacc ggcggatatc cgcagccagc tctgccgcca tttccccgtt ttttcgtaat
     6661 ggctgaggct ccacaataat tcccctggca ccaatcaccc gctcttccag cttgtcaaac
     6721 acaccaatca ccaggtcatg attgatatcc agaaaacggg cctgctcccg cagggaaacc
     6781 gcaccgtatt tactgagctg atcagcagag cgattttccc gccgggcttt atgtgtccgg
     6841 gtcggtttca ccgcctcata ggccatgatt aacgcccttg aacgcagtct ggctgctttc
     6901 catccggggg aaaacacgcc tatcacatca tcaataattg ccattaaaac ctcgccagtt
     6961 taaatcccgg ttttccccgc ctgcggctca ccatcgcggc aagcctgcgt tcccactcct
     7021 gacgtccggc gcggatctga gaaaggcttt ccagcgtcag ttgctgcccg ttgaaggtga
     7081 cagacttccc ctccagtacg gccatttccg cttcacggta ccgctgtatc atttctctgg
     7141 cttcttctgt gctcacaacc agcctcctga tgttgtccat ggattatctt ccgcacgctc
     7201 cgtccgcagt tttttcttcc ggcgacgacg tttttctgcc ccggccgtca gttccgggga
     7261 taccgtttca ccagaacgct cctgcgggaa gacgagccac gtttcccgct gtgcccagtc
     7321 cggtgcggag ggccagcgga tcttttcgta accatgcaga acagcaagcg catccgcata
     7381 aaccagcagg tcaaacgcct cgttagcgcc cctgcccggt tttcgccatt ttccgtcact
     7441 gccgcgctct tcataggtca gctcatcgta aaaccaccgc cccagccagt cgggaaagtg
     7501 gatatagttc ggccctggtg tgtcacgcca cagggcatta tttacacggt ccttaaacgc
     7561 atccgtctga accagccaca gcgcgacatc gccactggct ctggcacggc gggcacttct
     7621 gccggtatta tccgggaagg tacggttaat cagcctgtca cggcgaagac catccccctt
     7681 gaacagaaac accctgttgc ccagtccgtc actccggcaa cgacgccaga aacgataggc
     7741 gttatctgtc accccggctt cccctcccgt atccaccgcc atggccatca gacgcatgcg
     7801 cacatccgga tcagaagcca gcggccatgt tttatggaac acatccgtca gcaacaaatc
     7861 ccagtcctcc ggatatgccg ccggatcaac cggcagactt tcaccgttgg gactgcagcg
     7921 cagtgaatgc cggatgttgt agcgatcaac aatccagcgt tccccctgct ctccgtatcc
     7981 ggtgatctgc acaacaaaac ggcgattttt accgccctgt acgtcaaccg ttgcctcaat
     8041 aaaacgcaca ccatccggca cagatctccg gggaaacggc tcggcacgct gttcaagcag
     8101 ttcactttta cgctgttccg tggctgaacg gggcagatag ggccgcccga tatcggtgtt
     8161 caccaccgct ttcagggtct cttcactgcc ggttcgctca tactcttctt ctgccgccag
     8221 cagcttaaaa atcagttgtt cccaggtctg aaacgccgca gccggcccct ccatccagaa
     8281 acttgctatt ctggaatttc ggggggttcc ggtgatactg ccatccgccg ctgcccgttc
     8341 gccttcccgc agccagatcc cctggttatt cagttcacgc ttctgttccg gagcaatcag
     8401 gccgcggcag tgcggacaca tcagacgggc agcctgaccg gcagccacaa aatccgggtt
     8461 attccggtaa ccggtcatgt tatccatcac cggctgaaaa tattccccgc agtgcggaca
     8521 cggccagtac caccggcggc ggtctccccg gttatacagt gacagaatcc ccgttgttgg
     8581 cggtgcctca tgtgcgccac cacaacgcca tttggtatcg gtgatatccc gccccggtga
     8641 actctcgacc agggtcatcc ccgaggacat aaaggtggtg gtacgctttg aggccagcgt
     8701 gaaggcatcc ccttccccgt ccacgttttc agggaaacgg tcataatccg tcagcgccac
     8761 acgacggtaa tccgaagagg aaaagacggt gatcgacggc cagccaatct tcaggaagga
     8821 gccgtcaaga aacattttat cgtggacgtt gttgtcatta cgggaaggac tgaggcgctt
     8881 gctgacctcc ggactgtggc gaaacgtcct ggaaagacgc gttctggaat gctcacgcgc
     8941 cttcgtctca gtcatctgca ccaccagcat atccgccgga tcacagatga tgccgtacac
     9001 aatccagcca tcaatcagcc cttcggtttt cccggttcgc gcaggtccca caaacaccac
     9061 cgcgtcatat tcacgggctg ataatgtatt aatggggtca atcatatagg gcgtcagcga
     9121 tgactcccac ggaccggaag tattggctcc ccgtggaacc cgcatataac gcctgatggc
     9181 ttccgctact ggtaaccggc ctggtgggcg aaacagcgag gccacttcgc gccagatatc
     9241 ggatgcgcgg ctatggctct cgttcacctg attcacatat cggcctcatc acaacagtca
     9301 atgactgcct tttccagtgt gtcgcggatc tcatcaacca caatctgtac ttcattcagt
     9361 tgtgatgcgg tccaccccct gtccctctcc agccggtcag gccaggtttc cagtacctga
     9421 actatcgctt tcaccacgac agaaaaggac cgcctgacat cactgactgg cacaagccga
     9481 ccagtttcct gctcaaattt cagtcggtca cgttctgact ggtaccatgc tttacgcgca
     9541 tgaggatcca tttcctcgtt atctacaggc agaggagctt tcatcagctc ggcaaggata
     9601 tctgtcagtc ggtacagttt gagattgctc tcatgaccac cggctgggct tatgtttttt
     9661 acccgagccg caacagtctg tcgatgagca ccggataatg cggccagttg ggaaatattc
     9721 agatgcagat tttttaattc acgatccata actccccctg aaaat
//
LOCUS       DAZKJR010000175         9741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKJR010000175 DAZKJR010000000
VERSION     DAZKJR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.176"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1136
                     /gene="pilO2"
                     /locus_tag="ACHOWS_004527"
     CDS             <1..1136
                     /gene="pilO2"
                     /locus_tag="ACHOWS_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFQ0050003.1"
                     /translation="LSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYA
                     ITELTLPGETPLYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPA
                     AFSAGAQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYE
                     RWQTEKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAE
                     CSTDGGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLS
                     ENAVTVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSLTDENGQIIQPPWKEYDLQM
                     QTTLPAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            1126..1578
                     /gene="pilP"
                     /locus_tag="ACHOWS_004528"
     CDS             1126..1578
                     /gene="pilP"
                     /locus_tag="ACHOWS_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFQ0050004.1"
                     /translation="MPDKAKTLLLSALLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEGGRQQESISAVTTNAQALPSPRLTAIFGVQPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            1663..3171
                     /locus_tag="ACHOWS_004529"
     CDS             1663..3171
                     /locus_tag="ACHOWS_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFQ0050005.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNTGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYHPDQE
                     CTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            3173..4273
                     /locus_tag="ACHOWS_004530"
     CDS             3173..4273
                     /locus_tag="ACHOWS_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFQ0050006.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            4347..4883
                     /locus_tag="ACHOWS_004531"
     CDS             4347..4883
                     /locus_tag="ACHOWS_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFQ0050007.1"
                     /translation="MNFRARLSSYFSLKKDKEDDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNTVD
                     PVTVCSSDKNNIKLETNS"
     gene            4937..5410
                     /locus_tag="ACHOWS_004532"
     CDS             4937..5410
                     /locus_tag="ACHOWS_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFQ0050008.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            5429..6064
                     /locus_tag="ACHOWS_004533"
     CDS             5429..6064
                     /locus_tag="ACHOWS_004533"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFQ0050009.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGHYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAAASTMICMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            6069..7301
                     /gene="pilV"
                     /locus_tag="ACHOWS_004534"
     CDS             6069..7301
                     /gene="pilV"
                     /locus_tag="ACHOWS_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFQ0050010.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCSPDGLVGRTSTGAILSCQSGVWKNN
                     ISDALVFDYSACYDAEEDSRKPVYANWGSGSNGFAVCDGKAGYGAAGFRAYRGKPGYS
                     GSMVWVKIRCCKLKVNTP"
     gene            complement(7307..7696)
                     /locus_tag="ACHOWS_004535"
                     /pseudo
     CDS             complement(7307..7696)
                     /locus_tag="ACHOWS_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            8048..9214
                     /locus_tag="ACHOWS_004536"
     CDS             8048..9214
                     /locus_tag="ACHOWS_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0050011.1"
                     /translation="MFQKKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHPM
                     ADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGTC
                     RMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEIL
                     SLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFKS
                     AWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLRA
                     YHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISHA
                     SVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            9256..9546
                     /locus_tag="ACHOWS_004537"
     CDS             9256..9546
                     /locus_tag="ACHOWS_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050012.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
BASE COUNT         2581 a         2568 c         2340 g         2252 t
ORIGIN      
        1 atctcagtaa tgaagatgcc gatacccaca ccatggtagc cactgtcagt cgccgcatac
       61 tgcccggaaa accccgtcag tgcttctccc tcgccctgct gatcctgcca ctacttgaac
      121 atggcggata tgccatcacg gaactgacgt tacccggaga aacaccgctc tatagctttg
      181 tttccgttgt cgacggtgtt ctcgtcagtg acctggtggg aagcggggaa gaggttcggg
      241 aagcccggga tacctttctt tccatcaaca ccgaaccgac tcagggctgg atacactatg
      301 aaccagcggc attttcagca ggcgctcaga atcagcacct gccgctggcc tccctgactg
      361 gctctggtaa acatccggct gccgcacgcc tgaatccggt atcccgggga ccgcagttca
      421 tcactgtctc tctgatcatc gcccttctcg gcactgcatg gtacggctgg cagtattatg
      481 aacgctggca gacagaaaaa gccgcgcagg cagcggttgc ccgacaggcc acagaggaac
      541 gaatcactcc cccctggccc ggactccctg aaacagacgc gttcatccgg ggatgtgctg
      601 atatatggac ttcactgcca ctgtctgctg ccggatggcg ttattcgctg gcagaatgct
      661 cgactgacgg tggcagcggc aatcttcgcg catcgtacac caatactgcg ggcgcgacag
      721 tgactgactt catccggcgc atcagtgata tgacagacag ccgaccgttt attgtgatgc
      781 cggaaggaaa gaccggcggc attggcctgc cggtcacctt ccggctgtct gaaaacgccg
      841 tcacagttga tgcactaccg gaaaccgctg tcctgcagga acgactgacc tcgctggctc
      901 agtcaatgca actgaaactg gactggcagg aagttaataa cagcctcact gatgagaacg
      961 gacagatcat tcagccccca tggaaagagt atgaccttca gatgcagacc acactccctg
     1021 cacaccatct ggctgaacgc ctgaaagagc cttctgtccg ttttatctct gtcacacggc
     1081 aactggagaa cggtcgtttc cactatcaat ttacaggtaa atattatgcc cgataaagcc
     1141 aaaacccttc tgctttcagc cctgctgtta ccggcaacgg caatggccac aaccggaaat
     1201 gcagagcaac agaacacaca gcaaataagg attgaacaga tacaggaaga gacagccctg
     1261 tatgaagcca tgctggcgag agagaagatc cgccatgaac tgcgaacact tgagcagggc
     1321 gttcccgcag agggcggacg tcagcaggaa agcatttccg cagttacaac aaatgcacag
     1381 gcattaccat ccccccgcct gacagcaata ttcggtgttc agcctcaact gactgccagg
     1441 atagaacttc cggggggaag tatctccgat gtgaaggcgg gccagaacat ccccggaacc
     1501 acttatcacg tggaaaaaat cacgccggac agtgtagtgc tggaacagtc tggcaccaga
     1561 aaaaccctgc gaccatgaga ctggccgccg taactggcgg ctgtcaaaaa taaaaggatg
     1621 aaagacatat tttccgggca ccgctgcccg taaggaggct ttatgctgga tccagcagat
     1681 ctgccgccgg atgatattct ggattatgtg gccatcgaca ccgacaacac cggacaactg
     1741 cggatcagag ttgtggaggg gaaaaaacat ctgcgtgccg tccaggaata tctgacccgc
     1801 cttcgcgcac gctatcaggg gcgtgtggga gactttgagt tcaccacgct ggatgtgata
     1861 gcccgcctca gacaggacgc cacaacgaca ggcgatgaaa acgctattaa cccgatacag
     1921 cagaaaatgc tcggctatat ccgccattct gcctcccttg gtgcctccga ccttcatatg
     1981 acgccagggc gggacaacac agacttcacc tatgtggaag cccgggttca tggcgaactg
     2041 gaagtgctcg atatcctccg gaaagaagaa ggtctggaat tactgggggc tacctattcc
     2101 ggcatgacgg atgtcatcaa gggcacacaa tttgatcccg gggttccaca ggacgcacgt
     2161 ctctctgaac agtacctgaa actggctggc ctgtttggcg cacgctactc ccactacccc
     2221 tgcgtgggag gcctctatgc tgttctccgt ctgatcaagg atgacagcca gcaaatcccg
     2281 accttcagta tgctgggtta tcacccggat caggagtgca ccctgcgccg tatcctgcaa
     2341 cgccctgaag gcattatcac cctctctggc cccaccggct cgggaaaatc caccacgctg
     2401 agaaccgcct ctgcggcata ccttgaacaa tacggcttca acaacaccgg cggtattctt
     2461 ctgccacggc gcaggttgtt cacgattgaa tccccgccgg aaggccgtat ccccggagca
     2521 attcagacag ccgttatgga taccacccgg ggctgggtgg actccatcaa atcagcactg
     2581 cgtcttgatc ctgacagcat cctgaacggt gaaatccgcg atcacgattc cgccattacg
     2641 gcaataaaag ccgccatgac cgggcatctg atgctgacca ccatccatgc gaacgacccg
     2701 attaacatcc tcgaacgcct tgagatggag ggtgttcagg ccaggatgat tgcagacccg
     2761 cagttgttca tcggactgct cagtcagcgt ctggttcagc tgatatgtcc ccactgtcgt
     2821 catccatgga atgacgtagc atcccgtcgt actgaggatg agcgacatct ggtggaaaca
     2881 ttctgtaatg ctgatgcggt atatctgaga aaccatgaag gctgtccaca ctgctggcgt
     2941 ggtgtcacag ggcgtacagt gattgcagaa gtcatctctc ctgacgccag attttttcag
     3001 ttataccggg aaaaaggccg tatcgaagct aaaacctact ggcacaggga actgggcggg
     3061 atgacgcgta atcagcatct gctgggaaaa atcaacagcg gactggttga ccctctggcg
     3121 gcccattaca tctctcccat tgatgaggac agctatacgc tgctgcgctg atatgaaatt
     3181 tctggaaaaa ctcaacagta ctgaactgga tctgtctgcc ctgaacagct ggacagccag
     3241 aaaaaccttc ggtcttaagc gtgccgagtt ctatgagtcc ctcggcggaa tgatacgtga
     3301 cggaacgccg tcactgcgcg ccctggagtt tatctgtaat gtcgaaactg actttggcac
     3361 ccggaaagga acatccggca tgtactttct ggccagagaa tgtattgcct ccctgaaagc
     3421 cagcggtatg ctgtcggggg ccctgcccac atgggttccc ccggaagaag cggcactgat
     3481 caggaacggt gaagaacgcg ggaacatcgc cgaagccctg ttccaggtcg caaaaaccgt
     3541 gaaagcacgg cgggaaatgg tgagcagtct catcacactc tgtctttatc cccttctgct
     3601 gctttcactc tgtgtggtga atatgtacaa cgcacatacc cgcatcatcc ccattgtcag
     3661 cgcttttgca ccggaggaga aatggtcatt ccagatgaaa gtccttgcag ccatgtcaga
     3721 gcttgtcatc ggctatggtc tgtggctcag tggtgccatc atcacgcttg tcatcatcat
     3781 ccgcttctca ctcagtcgct ataccggccc gggacgcagg cttcttgaca aactgccacc
     3841 atggtcgctc tatcagactt ttcatggtgt gaattatctg ttcagcatat cgtcaatgct
     3901 gcagataaat attccgcttt cacaggcgct gctcagaatt gaaaaaagtg cagagcataa
     3961 taaatggtta aaggagagaa ttcaggcaat aagaaaaaat gtattatccg gccagaatct
     4021 ggccaccgcc atgcgcaatt ctgactatga ttttccttca aaaatatgca tcaataaact
     4081 tctcttaaat tctgaaaggg aagacagcgt atctgcaatg aatacctatg ccggacagtg
     4141 gctggaagat gcaaaaaaga gcgtcagaaa aagtggatta attatcaccg gtatttcagg
     4201 aggttttgta ttctttttta ttcttaacat gattagcgcg atatacagta tcagcgatat
     4261 gctcaagcaa taatacctgc cgtaacacta aaacgccctt caatatactt tcattaacgt
     4321 aacaaccatt aatacaggag tcatatatga acttcagagc acgtctgtct tcttattttt
     4381 ctttaaaaaa agataaagaa gatgataaag gtttaacatt attagaaatt gccggcgcaa
     4441 tgattatcat cgcaatagtt gtcgcgctcg cgatgagcca gatgtccagc acaatggcct
     4501 ccagtgacaa ccagcgcgaa ctgaaaaacc tgcagacaat tgcaaccaaa atgaagcaat
     4561 acaagttcca gggaagatat accggaacgg attatatcaa aaccctgacg gaaagcggtg
     4621 cattgcctgc tgatatgatt gcaggaggca acaaagccaa aaacgcatgg ggtggtgatg
     4681 tcacgattgc tgccacagca gacaaatatg gctacattat caccagtaat aatgttccta
     4741 aaacaaactg tgttgagctg attaacagcc tgcgcagttc gtccatattt accaaaatta
     4801 tgaacacagc cccgaacacg gtggatcccg ttaccgtctg cagtagcgat aaaaacaata
     4861 tcaagctgga aaccaacagc taatgccctg ccacacagca ctccctccgg ggtgctgttt
     4921 cccggagcac ataatcatga aatggtttta tctctcatct cttttcttat tctgtctccc
     4981 ggcaactgcc gccgtaaaca gtaacacact ggatagctgc tttattcagg caggaaaacg
     5041 ttactccatc gatcccggac tactgaaagt aattgcccgt aaagaaagca gcctgaatcc
     5101 ccgggccatc aatcataaca gaaatgccag cggaaaaatt atcagcacag attatggcct
     5161 gatgcagatt aattccacac atattcccgg actgaaaaga atgggaatta tccgaagtga
     5221 aaatgaactg ctcagtaatg tttgcctgaa tatacagaca ggtgcatgga tccttgcccg
     5281 gcattttcag cagtgtggtg tgaactggga gtgtctcggt agctacaacg ccggatttaa
     5341 taaaaataac actcaccgca gaatgaaata tgcccggctg atttatgccg catatatgca
     5401 gggacgttaa taacggagaa tacgagccat gttcctcatc atcttactcg tgatctccgc
     5461 tacgctgacc atacagttac ttgccatcag actggccgga cattatctca tgcaggtgca
     5521 taccctccgg cgtccacgtc acctctccct gctctgtgct gttctggcac tcaccgccac
     5581 cattacgcac atcatacggg ggacagacat taccggactt tgctttctgt tgctgatggc
     5641 cggctgcatg acactgatga cactgactga tgcagcctcc tgccgtcttc cccgtccctt
     5701 caccctcaca ttcctgatgc tgggtgtggc gtttcgttta tggcagggcg atgctgctat
     5761 tgcgcttgcc tgtgctctgt tctggtttct cctgttatct ctcctgaggc gctacacctc
     5821 ataccgcaag ggagtggaga cgctgggact gggggacgtg tttctgattg caggtgtggc
     5881 catgtggtca tctgccaaca gcactccgtg gatcatcggt attgccgcag ccagcacaat
     5941 gatctgtatg tcgctgttcc cgcgcagtaa tactggggcc gctattccgt tcggtccgtt
     6001 tatctgtgca tcactctaca ttttcacttt ttttcctggt gccatttttc acaccggagg
     6061 gtaacaccat gcaaaaagac gacgataaag gttttgttct gtttgaaatt ctggccggac
     6121 tgattgtgat cggtatcgcc acaccaatga tttattcaga aattgaaaac tggctgaatg
     6181 agcagcttta ccagagtgct gcatatcatg cagatgccta caataccgca gccagaaact
     6241 atattgccga caacaatgcc cgtctgcata gcggcagcct tccggcaaat ttcacggctg
     6301 atgacctgat tcgtcaggga tatctgaaac aggggtttaa ccattctccc tttggtcaga
     6361 gttatatcac cggtatccgc cgtaaccaga ccaccgggcg tctggaagcc ctgacctgct
     6421 caactggagg gcagacaatc aaagaagaag ggctgcgtag tgtggcgggt cagttgccgg
     6481 ggctgggtgg atttatcagc aaaaatggaa cggcaacagg cgcattcgga gcctggacag
     6541 ataaaccggg agactacgga cttacctgtt cgacaggtca catcgccgtc gtcatgagcg
     6601 gtgatgacct gcaggaaagt gaccgtctct accgttttca ggtagcaggt cgtcctgaac
     6661 tgaatcagat gcataccgca atcaatatgg gcgggaataa cataaacaac accggcaata
     6721 tcaatggtca gagtgccaca ctgaaaggtg atattacgag tgaagacggc tggctaatca
     6781 ccagaaacaa taagggatgg atgaatatca cacatggtgg tggattcacc atgacggaca
     6841 gccagtggat acgcgcggtt aatggcaaag gcatcacaac caccggggaa atcaaagggg
     6901 ggaaagtcag tggtggtaca gtccggtctg acggtcgtct ttcaaccggg gagtatttac
     6961 agcttgataa aacagctacg gcaggcacaa agtgttcacc ggacggactt gtaggccgca
     7021 ccagtacagg ggccatactt tcgtgccaat ccggtgtctg gaagaataat atcagcgatg
     7081 cactggtttt tgattattcg gcctgttatg acgcagaaga agactccaga aaaccggttt
     7141 atgcgaactg gggtagtggc tcaaacggct ttgccgtctg tgacggcaaa gcaggctatg
     7201 gtgcggcagg attcagagcg tacagaggta aaccggggta ttcgggaagc atggtctggg
     7261 tcaaaatacg ttgctgtaag ttaaaagtta atacgcctta aatgcatcag cttgctttga
     7321 cgatgtagtt aaaggcaatg tttttggggc gcatttccgt tgtgcttccc tgggcccagt
     7381 aaggcatacc gctgtgatgc cctttcagcc atcccccacg ctcatgatag acaccggatt
     7441 gatcggcctg ataactcagg agatgtctgt tccgatctcc atccagccca cgtccgttgt
     7501 cccagccacg gataaaaaca ccgcgcaaat ccggcagctt acacccggga taagcacggg
     7561 caagctgagg ataccgggaa catgaaaatg actgcccgtt catcaccagg tagccttgtg
     7621 gaggggtggc tgatggccac ggtatagggc ttccgacagg gtaggattca aaacctgccc
     7681 acataccgga ttggcacgct ggctactgcc tgacgcttaa ttaaataccg gccccaggta
     7741 tattgagaca cgtaacagat atcgttccac ctgactcata attaccccac gcataattat
     7801 tgacatacca gacattattt ttatcggaat aaacctgctg aaaaccaccg aaaggagaac
     7861 cattaagata cgtccatgca caatatgcat ggacaccaag tctgaaatta cgaatatttt
     7921 ttccgatgtt ataggtctgc gttgtgaatt taatttcagt tgaccgccag acaccggatt
     7981 ggcacgaaag tattgcattt ctgtatccag cctcctacct tccccgtttt tatcgtggaa
     8041 ggcagatatg tttcaaaaaa aaactcgcaa aatgtccctc agccgggcac tggacaaata
     8101 cctgaagact gtttccgtcc ataaaaaagg tcatctgcag gagttttatc gcgtcaatgt
     8161 gattaaacgt caccctatgg ccgatcgcta tatggatgaa atcactacca ttgatatcgc
     8221 cggttaccgg gatcagcgtt tagctcaaat taacccacga acagggcgtc aaatcaccgg
     8281 aaataccgtc cgtcttgaac ttgccctttt atcatcatta ttcaatatcg cccgtgtgga
     8341 atggggaaca tgccgtatga atcctgttga actggtccga aaaccgaaaa tcagtaatgg
     8401 ccgcgaccgc cgactgactt ctggtgaaga acgccgcctt tcccgctatt tcagggacaa
     8461 aaatccgcag ctctacgtta tcttccatct ggctctggaa acagcaatgc gccagggaga
     8521 aattctctca ttacgctggg aacacctcga tttgcagcac ggcgtggcgc atcttcctga
     8581 aacgaaaaat ggctcaccac gcgacgtacc actctcccgg aaagcacgta actacctgca
     8641 aattctgcca caacagataa acggcaatgt gttcagttac acctcgtccg gtttcaaaag
     8701 cgcctggcgt accgctcttc tggatctgaa aatagaaaat ctgcattttc acgatctccg
     8761 ccatgaggcc atcagccgtc tgttcgagct cggcacactg aacgttatgg agattgcagc
     8821 catttccggt caccgctccc tgaacatgtt gaaacggtac acacatctcc gggcatatca
     8881 cctggtcagt aaactcgatg caaggcgtaa acagaccagc aaaatttccc cttattttgt
     8941 tccctaccct gcaactgtca gaaacagaaa cggagtgttc attgtgacac tgcacgattt
     9001 cgaccttgaa agccgtgctg aaacccggga actggcaata tctcacgcca gcgtattgtt
     9061 gctgcgcgca ctggcccagg ctgctcagcg gggtgaacgc gtccccacac caggcgagct
     9121 acccgcaaac attgatgaac gggtcatgat ttgtccgtta acaaacagcc agttcatgac
     9181 aaaagcgaac agtattttca tatcctcctc ctgaacaaat aatttttgtt caaaattaac
     9241 gctatgagac cctttatgaa acacttatcc tcccgtgcag ccactgcgga taataatcgt
     9301 atcgttacaa ttgaagacgt ttacacaatc gcattctcgc aaccaattca caccgaatca
     9361 gcgttggggc aaaggtggat gttgatccat actggtgaat atgaaagtgc cagcgatgca
     9421 aaacgcttcc ttgatacgct cgtcaccggg cactgtgctt gcatggtaac accatcacct
     9481 gttctggatc atgacacctg gctgcgtatc cgcctgctga tgggcgagat aagggatgcc
     9541 aaataaacat tcatggcccc aacacataaa aataacgccg gggactctgg cagaggtacc
     9601 attcttttta catagagcca atatataaac aacttttcta tgataatatc tttatcatgt
     9661 tataaataat tgccccctca gcagaaaggg cccaaataga aaaagcaaaa atccttataa
     9721 aaatattctt tacaagcgtt c
//
LOCUS       DAZKJR010000176         9724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKJR010000176 DAZKJR010000000
VERSION     DAZKJR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.177"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(260..1633)
                     /locus_tag="ACHOWS_004538"
                     /pseudo
     CDS             complement(260..1633)
                     /locus_tag="ACHOWS_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1832..2878)
                     /gene="fbpC"
                     /locus_tag="ACHOWS_004539"
     CDS             complement(1832..2878)
                     /gene="fbpC"
                     /locus_tag="ACHOWS_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFQ0050013.1"
                     /translation="MSQKNFVELRNVTKRFGSNRVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKDALTMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTAGVR
                     PEAITLNNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(2890..4968)
                     /locus_tag="ACHOWS_004540"
     CDS             complement(2890..4968)
                     /locus_tag="ACHOWS_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0050014.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMNVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILTVWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPLYLAGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(5037..6068)
                     /locus_tag="ACHOWS_004541"
     CDS             complement(5037..6068)
                     /locus_tag="ACHOWS_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0050015.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(6065..7381)
                     /gene="uhpC"
                     /locus_tag="ACHOWS_004542"
     CDS             complement(6065..7381)
                     /gene="uhpC"
                     /locus_tag="ACHOWS_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFQ0050016.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLNANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(7454..8995)
                     /locus_tag="ACHOWS_004543"
     CDS             complement(7454..8995)
                     /locus_tag="ACHOWS_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFQ0050017.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALLILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPAILAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(8995..9624)
                     /locus_tag="ACHOWS_004544"
     CDS             complement(8995..9624)
                     /locus_tag="ACHOWS_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0050018.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWPALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
BASE COUNT         2406 a         2643 c         2543 g         2132 t
ORIGIN      
        1 ataataattt tattaaagtt gagttgtaca acaatgtggg agagaaataa tctgaccgga
       61 ttacagctat ttgatgagtt tgagtacgat attgatattg gtttcagtta ttgatttatt
      121 tatgtcataa cttttctgaa agatagctgg taattgcctt gagaaagttt ttatatgtta
      181 ttctgtaaaa caggataaca gaaaataatt atgtctgaaa taagtagatg atgtcccctc
      241 tgagctggct gtctcttgat cagatatcct gatcaagaga cttcatcacc aggtaaccct
      301 caaccatatc ctgaagtctg aaccagtcat cccacataac tacccaaccg gggcggcctg
      361 tgcgtttgct gtcatgtcat cgccccagtt tagccagttt cagactggct catttcagga
      421 tcggtgcctg cgccggaagc ggctctcctt ccagcttaac ccacagcagt ttccactctg
      481 tcggcgtcag tattttctca caactgtcat tttgtgtttc ttcactgaca cctccctgcc
      541 gcaggcccgg cacccgtacc gcgataaacg ccttgataac caccatgcgc tcaaggttat
      601 cccgggtctg aattcgcagc gattccacac atgtaccacc acttttccac gccttgtggt
      661 attcctctat cagccagcgt cgctcgtaat ggctgacgat acgtcgcgca tcggcggcac
      721 tcgccacttt ttctgacgtc agcagatgcc agcaggcact gtcctctgcc tgctcccggc
      781 aacagacata cgtgagcgag agcgcctggc cgctgttgtc gggatttttt atgctgactt
      841 cgctgtaact gatgaacatc cgggcctggc gggctgcccg cccgcctttt tgcatcacat
      901 tcagcgtgtg gcttcccgcg gttgccagga cttccggcag ttcgaagagc ttgccgggtg
      961 cttcttccag ccggctattc tgtgcagcac gcaccacgaa gcgctgtccg tggctgactt
     1021 tataatgcag gtaatgccag atatccgctt cccggtcaca gacagtgatt acccgtttct
     1081 gtatctcccc cagccgttcg gccatgcgct ccgaagcctg ctgccagcgg taactttctt
     1141 tttcttcaca gggacgttct tttcgctggt gcttaacacc gtaggtgtcc gtggcccgac
     1201 tccagctctg ctgttcgata agagcgactg gcagggcgct gtcgggggcg tatatcagga
     1261 cagagtgagc cagcagcccg cgcgtcttcg ggttagtggt ggtattcccc aggtcatcag
     1321 atgccgtact gtggctgaag ttaatggtgg aggtgtcttc cagtgcgggg agcagcggat
     1381 gtgcctcaca tgcccttgca gtggcggtaa atccggctcc ggaaatggct tccggggaca
     1441 ctgacgggtt acgcatcagg cggtacgcac cttcaacctg agcagtggag tgggattatt
     1501 tcacaataga aagacctgca tgctgagcga gagaagaggc cagtgacaca aggcgtcgtg
     1561 tacgacgcgg atcaccgaga cgggcatgtc cagactgctc gttagcccat gaataacaat
     1621 cagaaagtac cataacagac ccgaagaaaa tgaaatataa gagaagatca acgggtgaag
     1681 aaaaagttca aaaaatcgct gccggggagg aaggaaacta ccggatggaa agagtccccc
     1741 ctaaagcaga ctgacagaca tcacaaatcc ccaggtggtg atttgtgtat aagagacagc
     1801 tgagctggag gggaccgctt atgcgctatt tttatgccgc atccgctaaa acaaacatcc
     1861 cgtaagggtg gatttcgagg taatagtgct caccaatatc gggctgtaaa cgggtggcgt
     1921 tgacttgcaa taaaatctcc tgaccatgcc attctacggt cacttcgtac tgcggcccca
     1981 tgtaagcgac atggcgaata acacaacgct gactctcttc gccgtgattg tttaaggtga
     2041 tggcttccgg gcgaacgccg gcggtgccgc taccttgcgc cgggaaatgc gctgcgcgcg
     2101 gcaggcgata accgtagata tcgacatact cttcgctaaa gttcgccggg aacaggttgg
     2161 cgtcgcccat aaaacttgcc ataaatcggg aggctggctg acgatagaga tcctgcggtg
     2221 agccaatctg catgatgtga cctttattca tcaccagcac agtgtcggac accgcgaaag
     2281 cttcactttg atcgtgagtc acgtagagtg acgtgatatt aaactgcttt tgcagctcgc
     2341 ggatcttatc gcgcatgctg cggcgcaggt tggcatcgag gttacttaac ggctcatcaa
     2401 acagcagcac ctttggcttg aggatcaacg cacgggccag agccacacgc tgttgctgac
     2461 caccggaaat ctggtcgaca tagcggtctt cgaatccttc cagatccacc attgtcagcg
     2521 catctttcac ccgctgcttc acttcactac gcgacacgcc aagcattttc agcccatagc
     2581 caacgttttc gcccagcgac atatgcggga acagagcgta tgactgaaac accatgcaga
     2641 tatcgcgttg ctgaatggaa cgatgcgtga catcttcgcc atctataaaa atttgccctt
     2701 cgctcggttt ttccagcccg gcaaccagac gcagaatggt ggttttgccg catccggaag
     2761 gaccgagcag cgtcaccatt tgcccttgtg ggatggtgag attgatattg tcgataaccc
     2821 tattactgcc gaatcgttta gtgacgttgc gcagttccac aaaatttttc tgactcatag
     2881 tgcgctccat tacgcctggt ttttggcttt agaacgggaa atacgtgctt caccgatcag
     2941 ccagtcaaag aggaaaataa tcgccagcat caccacgatc agaatcgaac cgtaggcgat
     3001 ggctacgcca tattcgccgt cttctacgcg gttaaggatg taggcggttg ccacgcgtgt
     3061 gtccggtgtg acgagaaaga cgatggcgct gactgtagtg atggcccgca caaagctgta
     3121 aatcagggcc gagagaatgg ccgggcgtaa cagcggcagt aaaatgtgcg taatggttcg
     3181 cagggaaccc gcacgcaggc taagtgaggc ttcatccagc gatttgtcga tctgccccag
     3241 acctgcaatc ccggcgcgaa tacccaccgg aacgttacgc atcaccatcg agataataac
     3301 aattgctgcc gttccggcga gatagagtgg ggcgctatta aaggcaagaa tatacgagac
     3361 acccgccact gtacccggta cggcaaagca cagcatggtg gtgaactcga tggtcttttt
     3421 gcctttgaat tgttgacgca ccaccaccca ggcgatgagc aggccaaaga tagcggtaat
     3481 gggggctgca atcccggcgt agagcagggt atccagcagt gaaggccacg cgccgtcgct
     3541 catcccctga ccaaataact tgataaagtt atccagcgtc agggtgtagt caacgcccca
     3601 gttcaccgtg aagctgccgt agaaaatgct gccgtagagc agggcgttaa aggcaatcca
     3661 taccgtaagt atggcgacca cgctccacac cagggtgacc ggtagcggct ggacgtcgcc
     3721 acggtaggat ttgccggaaa ccgtgacgta agagcgttta ccaatccaca tatactggac
     3781 gcagaacacc agcagcgaga acagcagcag gaaagcacca agcgtgctgg cggcctgata
     3841 atccagctgc gagccggtaa tgtagaagta aatttgcgtc gccagtacgt cgaagttgcc
     3901 gccaagcacc agcgggttac tgaaatcagc cagtgactgc accacgacga ttaaaaaggc
     3961 attggcgagc gcgggcttta gcaatggcac aaacacgccg ttaaaggttt gccagcggct
     4021 ggcgcgcagg gtataggagg cttcttccag cgagggatga atggttttaa tggccccgtc
     4081 gaggatcata aaagccattg gcgtgaatgc cagaacctgc gccagccaga tcccggtaaa
     4141 accatacagc cagttggtgt tggtcagccc aaaccactcc accataaact ccgttatgta
     4201 gccggagcgc cccatcatga gcgtcacgcc taagccgacc acaaaaggtg gcgtaacgat
     4261 gggcagaatg gaaaaaatgc ggccaataat ggcgctgcgt ttggcgatgc gcgtggtata
     4321 aatcgccagc accaggccga agaaggtaca gccaataccc accgctatcg acaagacgat
     4381 agagttgacg atcacctgca caatatgtgc ctgcgagagc acgttcataa acgccagcgg
     4441 ggcgaactct cccgcgtcgt tggtgaacat cgggataaaa atggcgatgc tcggccagac
     4501 gataaaaatg ccaatcagcg ccacaatcgc aatcagtgag ccgatcacga agcggtcgcc
     4561 gcccagccat tcgaggcggg ttaaggccag cgtcataatc gcgcctaatt caacaaacag
     4621 cacgatggcg gcgtagccta aaccgcgtcc agccacggtg gcgctgatga cgataaacgc
     4681 catacagagc aatgcccagc tggcatcaaa ataatgacga ctacgctgtt cgcgtgtggc
     4741 ctcacgaaac ggacgaacca gcaacagact cggtaataaa taccacagcc agctgatatt
     4801 aaaatgcgac cagccataag cctcgaggat ctcatcactc gtggactcca gcaggccata
     4861 atcaaggctc cagctcggca acaggataaa tgccagccag cccagcaaaa cccaaaggaa
     4921 aatcgcatcc cgctttttca cgggatggag tgcaagtgtc tgtgacatag cagttccggt
     4981 atggttggcc ggggcaggcg tttgtgctgc acccggcaaa tgcgcctaat gcgggtttat
     5041 ttacccatct tcacttcgct gacccattta ttaatcaatg ccttacgaac atccgtggaa
     5101 ccatatttat ccatgtcgta gttgattaat ttcaggtcgt cgagcttcag cgaatttggc
     5161 gacgtttcgg cggtggtatt ggtcaggatc tgataagact tccctttctt ccacgccagt
     5221 tcctgagctt cttttgataa tacccagtcc acaaacagtt tggcgttgtc gaggttgcgc
     5281 gcgcctttca gaatgctgac gccgccaatt tcgtagcccg taccttcaca cggggaaatc
     5341 aactccagcg gcgcgccctg ttctttttcc agcgagtagt catgcaggaa gccaatacca
     5401 atcgccgttt cgccacgggc ggcgttacgt gccggggcta tgcctgattt ggtgtactgg
     5461 gagacgttgg cgttcagttg ttttagataa tcaaaggcct gatcttcccc ccacagctgc
     5521 gcaaaagtag ccagggcggt gtaggcagta ccggaacttt gcggatcggc aatttgaatt
     5581 tcgcctttat attccggttt ggtgagatct ttccagcact tcggcaccgg caggtttttc
     5641 tctttcagac gatcggtgtt tacgccaaaa ccgaggatcc ccacatatac ggcagaggac
     5701 agattccctt tcacttttgc cggatcgcgg aatttctcca tgatctgctc aaggttaggg
     5761 gatttatacg cctgtagcag gcccatctcg ccagcctgcg attgcgggtc taaagtgccg
     5821 ccgtaccaga cgtcagcctg ggggtttttc ttttcagcat cgacttttgc cagggtgctt
     5881 ccggaaccgt tgcggataaa cgaggttttt acatcatatt tctcgccaaa cgctttggtt
     5941 tcggcctcac acatttcatt ggtggcgctg caatagacca ccagccggcc tttcgcctgc
     6001 gcggcacccg ttaaagtcgc cagcgcaacg gcagaagcaa tcagagtaga ggtaaaggtc
     6061 attttcattg ttttatcctt cgagcgtgag tggtggtgat gcccgccatc agtaacggca
     6121 tcagcagtaa acccataagt actgcggcga ccgagagtaa actgaacatt cccgaccagc
     6181 cgtagcgttc gatgaccagc gacagtggcc agcccgcaag cgccgcgcca aggtaagcga
     6241 acaggccgag aaatccggtg attgaacccg ccgcagcttt gtgcccacac tccaccgccg
     6301 caagtccaat cagcatttgt ggaccaaaga cgaaaaagcc cacggtaaaa aagcacactg
     6361 ccagcagagc gtagtgatga accggtgcca gccacagtgc ggcgactgac accatcaacc
     6421 ccagcgtgaa cagcaaaatc attggcgctc gttgcccgct aaacagcaaa tccgatcccc
     6481 atccggcaaa taacgcgccg agtaagccgc ccacttcaaa cagcattacc gtggcgttgg
     6541 cgttgagcag gttgacgccg tggctttccg tcagccagat attgccccag tcgttaagcg
     6601 caatccggat cacataaacc agtacataag agacgcccag tagccagatc agcggatttt
     6661 gcagcatggt ggtgcgcaac atttgccaca gccccatcgg cggactttgt tgttcctgac
     6721 gtaactccag cggatcgtga cgccagtgac caactgtcgg taatccctct tcctggggcg
     6781 tgcctttgag ttgtagcgtt aaccagatcc ctaatgccat gctgattatc cccggtgtca
     6841 gcatcgcggc ctgccagccc caccagtggg cggcaaaggc gctaatcagc ggaataatcg
     6901 ctccgccaat gttgatcgac atattccagc agccccacca gaaaccgcgc tcattgcgtg
     6961 agtaccagtg ggtcagcaag cgggcacacg gtggccatcc ccagccctga aaaaaaccgt
     7021 tcagtgtcca gaccacaagc agaagcgtaa gtgattcgcc aaaggcaaac accacgttca
     7081 ataacccggt ggcgaacaag ccgacgccca taaaaccgcg ttgtccgtgg ctgtcatgcc
     7141 acagtccggc ggtaaatttc gacaatccgt agctgagata aaacagcgaa cccagcaggc
     7201 caatgtcgcc tttatccagt ccgaggtcgg tttgcagcgc cggaaggacg taattcacgc
     7261 tttttcgcgt cagataaaat gcggcgtagc caataatcat gcacatcagc aatcgcggac
     7321 gtaatgtccg gtagcattga ttaatttctc tcgctgaacg cgcgtgcatg gttgtctcca
     7381 tgttgctgac tatatgaaaa tgaaagtgaa aggggatgag agaaaatccg ggatacctaa
     7441 gactttttcc tggttatccg gcgatttgtt gcaaaaatgt gggcaagtta acaattaccc
     7501 gcgtgccgtg ctgtttttcg agcgttaagt cgccgcccag ggcgctgacg cgctcacgca
     7561 tcccctgaat gccaaatccg gccattttcc cgctggcgat ccccacaccg ttgtcgctga
     7621 cctccagatg caatacctct ccctgctggc gcaggataat cgtcacttcg ctggcttcgg
     7681 cgtgtttaca gacgttattg agcaactcct gcaataaccg atacaaggta aagcgcaccg
     7741 tttcgttttc tggtgtggag ttcagttgat aggcaaactg gcaatgaatg ccgcgttcac
     7801 taaaggcaaa ttcattaatc agatgcagca acgcttcgcg gaatgtcagt tcatcaagcg
     7861 cgggaggccg caactggcgc agcaactggc gggtggagag atgaatgcgg cgggcgagtt
     7921 cattgatttg gctggctgcg gattgaattt gcgccggatc gcgcgcccgc ttcaccagtt
     7981 gcgactggat ctgaatggcg gtgatgttct gaccgatttc gtcgtgcagc tctcgcgcca
     8041 ggtttttgcg cgtgtcctcc tcggtgtgga tcagcttttc cgccaatgcc cgccgcgctg
     8101 ccagctcggc ctccagccgt tgtcggtaat ggtgcaggtt tagcgccaga tgttgctggc
     8161 gactgatggc aatgcccagg ccaattccca gcaatgcctg ggtcgcgagg aaaatctcca
     8221 gctccaccag gttactaaag cccacgccaa tctggcgggc gatggtgatc atcatgctgc
     8281 ccaaaagccc ggagagtacg ccaccttgcc agccaaattt ccacgccatc accacattgg
     8341 gtagaaaaac tacgatcaac agcaggcgtt caatttcagg tgacagcacc atctgagtgc
     8401 cgataccaat gataaaaaac aggctacacc agatgattaa agaggtacgc aaggggggat
     8461 tatgcgtgtc aagccccagg agctggtagc gatgctgctg gcgcagaaac tcaaagatga
     8521 gatagacaaa cggcgtcagt aatacgccgc cggtaaatga ggtcaacccc ggcagcatta
     8581 ttgggcctga cataaaaggg gagagcaggg cggtattcaa caatgaagct gccgtgacag
     8641 tggctaacag caacgtcaga cgctgccagt aaagtggaaa acggtgccag aagttttgcg
     8701 caaggatggc aggaaacagg ctaataaccg gcgttgccag catgagataa ccgttcaata
     8761 gctgttcact atggagccag aacatcatca gcaaaggtgg gagcaccagc gcaggccagt
     8821 agcggcgaga gagcaaaata agaagggcga ggtaaacccc ctgcgggaga aacaacaccg
     8881 cctgttgacc gttattggtc agataaaagc tgagcgtcca cagcatcagc cagccagttc
     8941 cccaggccag cacaataaac agagaaatca cccaatgacg aaggttgcgc gacattaatg
     9001 ccccgccagc agatggtggt cgagcgcaaa atgcaccagc tcaatggtac tatggcagtt
     9061 cagttttccc agcacattgg cgcgatgcac atgtaccgtt ttatgactga gttcgagctt
     9121 aaaggcgatc tctttaacgc tgtcaccttt gaccagtaaa tcaaacactt cacgttctcg
     9181 tggggtgaga atttccagcg cctgggcggg ttgcccgccg ccgcgtaaag cccgaattgc
     9241 atcggcacag agataatgcc cgcccagccc gaccgagcgc accgcctgta ctaactcttc
     9301 cggcccacag cgtttggtca aatagccgct ggcaccagca tccagcgcgc tttgcacaaa
     9361 ggttggcgcg tcataaatgc taagaataat ggcgcgaaat tgcggttttt gtgcacgcag
     9421 gcgcttaagc aaactgaggc cgttttcgtc cggcatagca atatcaatga cggcaacatt
     9481 gacgtcatca cggattaatg ccggccatgc ctgtgcggca ctactgtact gaccaatgac
     9541 ctccagatcg tcttccagag agagtaattg tgcgaagcca gagcgcacca ccacatggtc
     9601 atccaccagc acaacccgaa tcatactttt cttctcttat tatctcattc ttctatcatg
     9661 catcacggcg cgtcgctggc aatcaaccag cgacagatat ggaatggatg taacaaaaaa
     9721 tgta
//
LOCUS       DAZKJR010000177         9534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKJR010000177 DAZKJR010000000
VERSION     DAZKJR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.178"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..1246
                     /locus_tag="ACHOWS_004545"
     CDS             26..1246
                     /locus_tag="ACHOWS_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HFQ0050019.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFICCIDSIENARALSAFFASQQQTLNVMIELGVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLSANSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            1514..3664
                     /gene="btsT"
                     /locus_tag="ACHOWS_004546"
     CDS             1514..3664
                     /gene="btsT"
                     /locus_tag="ACHOWS_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFQ0050020.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            3714..3917
                     /gene="yjiX"
                     /locus_tag="ACHOWS_004547"
     CDS             3714..3917
                     /gene="yjiX"
                     /locus_tag="ACHOWS_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFQ0050021.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            3928..4884
                     /gene="yjiA"
                     /locus_tag="ACHOWS_004548"
     CDS             3928..4884
                     /gene="yjiA"
                     /locus_tag="ACHOWS_004548"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFQ0050022.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEVEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPSRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDKIRAAFAGLRK"
     gene            complement(4918..5220)
                     /locus_tag="ACHOWS_004549"
     CDS             complement(4918..5220)
                     /locus_tag="ACHOWS_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFQ0050023.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(5235..5336)
                     /locus_tag="ACHOWS_004550"
                     /pseudo
     CDS             complement(5235..5336)
                     /locus_tag="ACHOWS_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            5533..6699
                     /locus_tag="ACHOWS_004551"
     CDS             5533..6699
                     /locus_tag="ACHOWS_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFQ0050024.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVDREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            6763..8382
                     /locus_tag="ACHOWS_004552"
     CDS             6763..8382
                     /locus_tag="ACHOWS_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFQ0050025.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEVLGYGE"
     gene            8372..>9534
                     /locus_tag="ACHOWS_004553"
     CDS             8372..>9534
                     /locus_tag="ACHOWS_004553"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFQ0050026.1"
                     /translation="MGSKWIEVSLGEISEKIGDGIHGTPTYNNSGNYYFINGSNLIDN
                     SIKITETTKCVDHDEYLKHRKKLSNNTVLVSINGTIGNTALYNNENIILGKSACYINL
                     KDNISKHFILYVLSGYLFQEYIQRCSTGSTIKNVSLKMMRDFRFLMPESKEEQEKVVR
                     IIQKIDELKRLNNAQNQTLEQMFQALFKSWFVDFDPVIDNALDAGNPIPETLQSRAEL
                     RQNVRNSTDFKPLPAEIRSLFPSEFEETELGWVPKGWESETFDSFCDLIQSGGTPSRK
                     ETSFWDGGTIKWLSSGEVKGKIVLDTKEKITDTGLLNSSSKLWEKYTTVVAMYGATAG
                     EVCIIGDKMAANQACCGLYSKTFPFFVYNFVCNKANELASKATGSAQQNLNKL"
BASE COUNT         2413 a         2370 c         2447 g         2304 t
ORIGIN      
        1 gtgaaaaact taaaaggtaa atgttatgaa ataccactca gaacctctgg ttccccataa
       61 atctgccctg atgcaaatgc ccgcaaatct ccttgccgaa gatgtctgtt tacctgccgc
      121 gatcattaaa aagcaggccc tggagaacaa cattacgtgg atgcaacgct acgctgacgc
      181 gcgcggcgtt tcactggcac cgcacggtaa aaccaccatg acgccgtgga tttttcaggc
      241 gcagcaggcg gctggtgcct gggcaatagg tgtcggtagc gcatggcagg ctggtgcggc
      301 aatggcgagc ggtattcagc gagtgctgat ggttaaccag ctggtcggca aggcgaatat
      361 gcagttgatt gcccagttgc aacgacatta cccgacggtc gattttatct gctgtatcga
      421 cagcattgag aacgcccgcg ctttgtctgc cttttttgcc agtcagcaac agacactgaa
      481 cgtgatgatt gagttgggtg tccccggcgg gcgctgtggt tgccgctcaa gggatgccgc
      541 actgacactg gctaaacagg cagcccaatt accggggtta cgcctgcggg ggcttgagtt
      601 atatgaaggc gttttacacg gtgacgatcc acaaccgcag gtagaagctc tgctgcggga
      661 tgccgcacaa ctggcctgtg atatggcaag tctggttgat ggcgagttta ttctcaccgg
      721 agcaggcacg gtctggtatg acgtggtgtg caatatctgg ctggcggcag aaaagcctga
      781 taactgccgc attgttattc gccctggctg ctacatcact cacgacaccg ggatctacga
      841 cgcggcacaa caacagttgc ttgcccgtga tcctgtggcc tgcgatctgg caggcgatct
      901 cacatctgcg ctggaactgg tcgccatggt gcaatcggtg ccggaagctg atcgtgcggt
      961 ggtaaatttt ggtaagcgtg attgcgcctt cgacgccgga ctgccgcaac cagtcgccca
     1021 ctaccgcaac ggcaaatcac tggcatttga tccacaggcg attcgcagta caggtattat
     1081 ggatcagcac tgcatgttgc aactgtcagc taatagcgac gtgcaagtgg gggatattct
     1141 ggtgtttggc acatcgcatc cgtgcctgac cttcgacaaa tggaaaacgt tgttattgac
     1201 tgatgacgac tacaacgtac tggcagaatt agacactctc ttctaagaac gccccttcgg
     1261 ttaattatcg aaggggcatt tttattgtga tctcaatcac atatccaccc gacgaataat
     1321 tcttgcccaa ccgctcaccg ccattcctga ccgcttacat ccctaaaata accactcagt
     1381 tatttacctt actttacgcg cgcgtaactc tggcaacatc actgcaggat agcggtcaat
     1441 ttacctcctc aaacgcaacg caaacctaga acggcttcgg ccaactatta atcaatacat
     1501 gccaggtttt actatggata ctaaaaagat attcaagcac ataccctggg tgattctcgg
     1561 aatcatcggt gcattctgcc tcgcggtagt tgcattacgt cggggggagc acgtcagtgc
     1621 cctgtggatc gtggtcgcct ctgtgtcggt gtatctggtg gcttatcgct actacagtct
     1681 gtacatcgcc cagaaggtga tgaaactcga ccccacgcgc gcgacgcctg cggttattaa
     1741 caacgacggt ctgaactatg ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc
     1801 cgctatcgcc ggtgctggtc cgctggttgg tccggttctc gccgcgcaga tgggctacct
     1861 gcctggcacg ctgtggctgc tggcgggggt agtgctggcc ggtgcggtac aggactttat
     1921 ggtgctgttt atctcctctc gccgtaacgg cgcatctctc ggtgagatga tcaaagaaga
     1981 gatgggacca gtaccgggga ctatcgcgct gtttggctgt ttcttaatca tgatcatcat
     2041 ccttgccgtc ctggcgctga ttgtggttaa agccctggcc gaaagtccgt ggggtgtctt
     2101 caccgtttgc tcaaccgtac caattgcgct gtttatgggt atctacatgc gctttatccg
     2161 tccggggcgt gtgggtgaag tctctgtcat tggtatcgtg ctgctggttg cctctatcta
     2221 cttcggtggc gtgattgctc acgatccgta ctggggtccg gcactgacct ttaaagacac
     2281 caccattacc ttcgcgctga ttggctatgc gtttgtttcc gcgctgctgc cggtgtggct
     2341 gatcctcgca ccacgcgact atctggcaac cttcctgaaa atcggcgtta tcgtcggcct
     2401 ggcgctgggt atcgtggtgc tgaacccgga actgaaaatg cctgcgatga cccagtacat
     2461 tgacggtact ggcccgctgt ggaaaggcgc tctgttcccg ttcctgttca tcaccatcgc
     2521 ctgtggtgcg gtatctggct tccacgcgct gatctcttcc ggtacgacgc cgaaactgct
     2581 ggctaacgaa accgacgcgc gtttcattgg ctacggcgca atgctgatgg agtccttcgt
     2641 ggcgattatg gcgctggttg ctgcgtccat catcgaaccg ggtctttact tcgcgatgaa
     2701 caccccgcct gctggccttg gcatcaccat gcctaacctg catgaaatgg gtggcgagaa
     2761 cgcgccgatc atcatggcgc agttgaaaga cgttaccgca cacgcggcgg cgaccgtcag
     2821 ctcctggggc ttcgtgattt cgccagagca gatcctgcaa accgcgaaag acattggtga
     2881 accttctgtc ctgaaccgtg caggtggtgc gccgacgctg gcggtaggta tcgcgcacgt
     2941 gttccacaaa gtgctgccga tggctgacat gggcttctgg tatcacttcg gtattctgtt
     3001 cgaagccctg ttcatcctga ccgcgctgga tgcgggtacc cgttctggcc gctttatgct
     3061 gcaagacctg ctgggtaact tcatcccgtt cctgaaaaaa accgactctc tggttgctgg
     3121 tatcatcggt actgcgggct gtgtgggtct gtggggctac ctgctgtatc agggcgtggt
     3181 cgatccgctg ggcggcgtta agagcctgtg gccgctgttc ggtatttcca accagatgct
     3241 ggcagccgta gcactggtac tgggcaccgt tgtgctgatt aagatgaagc gcacccaata
     3301 catctgggta actgttgttc cggctgtatg gctgcttatc tgcaccacct gggcgctggg
     3361 cctgaaactg ttcagcacca acccgcagat ggaaggcttc ttctacatgg caagccagta
     3421 caaagagaag attgctaacg gtactgacct gacggcacag cagattgcca acatgaacca
     3481 catcgttgtg aacaactaca ctaacgcggg tctgagtatt ctgttcctga ttgtggtgta
     3541 cagcatcatc ttctacggtt tcaaaacctg gcttgcggtg cgtaacagcg acaaacgtac
     3601 tgacaaagaa actccgtacg ttccaatccc ggaaggcggc gtgaagatct cttcgcacca
     3661 ctaaccgtgt ttagccccgc ttcggcgggg ctttgttctt atcagagtga actatgtttg
     3721 gtaacttagg acaggcaaaa aaatatctcg gccaggcggc gaagatgttg attggcattc
     3781 cagactacga caactacgtc gagcatatga agaccaacca tcccgacaag ccgtacatga
     3841 gctatgaaga attcttccgc gaacgccaga acgcgcgcta cggcggcgat ggtaaaggcg
     3901 gtatgcgctg ttgttaatgg agagagcatg aacccgattg cagttaccct actcaccggt
     3961 tttttaggcg cgggcaaaac caccctgctg cgccatattc ttaatgaaca gcacggctac
     4021 aaaattgccg tgattgaaaa cgaattcggc gaagtctccg ttgatgatca actgattggc
     4081 gaccgcgcca cgcagatcaa aacgctgacc aacggctgca tctgctgttc gcgctccaac
     4141 gagctggagg acgcgctact cgacctgctg gacaatctcg acaagggcaa tattcagttc
     4201 gaccgtctgg tcattgaatg caccggcatg gccgatcccg gcccgattat tcagaccttt
     4261 ttctcccatg agattttatg ccagcgttac ctgctggacg gcgtgattgc gctggtggat
     4321 gcggtacatg ccgatgagca gatgaaccag ttcaccatcg cccagtccca ggtgggctac
     4381 gctgaccgta ttctgctgac taaaaccgac gtcgcaggcg aagtagagaa actgcgtgaa
     4441 cgcctggcgc gcatcaacgc ccgcgcaccg gtctacaccg tcacccacgg tgacatcgac
     4501 ctgggcctgt tgttcaacac caacggtttt atgctggaag aaaacgtcgt cagcaccaaa
     4561 ccgcgtttcc actttatcgc ggataaacaa aacgatattt cgtcgattgt ggtggaactg
     4621 gattacccgg tagatatcag cgaagtttcc cgcgtgatgg aaaacctgct gctggaatcg
     4681 gcggataaac tactgcgtta caaagggatg ctgtggattg acggcgaacc tagccgcctg
     4741 ctgttccagg gcgtccaacg cctctacagc gccgactggg accgcccgtg gggcgatgaa
     4801 acaccgcata gtacaatggt gtttatcggg attcagctgc cggaggataa gattcgggcg
     4861 gcgtttgcgg ggttgaggaa gtaattttag atgatggccg ccatgtggcg gcctgaatta
     4921 ttttttattc tgatcgcgca gcatcgcttc acgtaaaatt gcgttcaggc gagtttgata
     4981 gcctttacct ggtcgcttca accattccat cacatcagca tcaatacgca ccgaagcctg
     5041 agttttcaat ggccgataaa acttaccgcg ttctgcgtta gaccattgct catcgctgga
     5101 aggtgggata tcactgtaat caatatcttc gtcggattta ttcgccagcg ccttcagttc
     5161 ggcctcatgc tgcacatcga gtgtagagga ggtccctcgt ttatgtttaa ccatgctcat
     5221 agcgattccg ttcgttgtgg atcatcaaac actaacaccg catcttcaaa gcggatgcca
     5281 tgtttcaccc gattaccttt ggccttattt gcatcccatt caaactccat tggcatagtt
     5341 gccatcccta attgtatata caaaaatgta tgtacaatta ttctgcaatt atagcgttga
     5401 tgttggaaat agcatcacgt ttcgaataat caaccatgaa attgacaatg tgtgagttgg
     5461 acttgattca tccaatagaa tttctcgcct gacatggcac actaaacacc tcgctgtcgt
     5521 taagggatta ccgtgaaaaa ctacaaagtc attaccccgc tttttcctac ctatgcgcaa
     5581 gttaaggcta tgatgaaagc cgtctcaggc tattccctga aagcggtgcg caatatgatt
     5641 actgccattc acgagcaaac cggaacgccg caaaaacccg ttgactggtc ggaacctgac
     5701 ctgtggatca gcgaacgctt aacgggtgaa gatgccgaca tcgcccgtcg tatttgggat
     5761 accgataatc acatattgaa cccgcgccac agctacggct gctacttatt cctcaactat
     5821 ccccagtttg agctgatgga aagcacgcca gacgatacct ggcagcccac ctcgcgcggg
     5881 caaaaattcc tgcaagatga cgaaaaaacg ctgcgttcac tggacgatca ggaaggcatt
     5941 ctgcaacttc ttgaactatt agctggccgc gaaatgtcgc gccgcgccga tttattgccg
     6001 gaatggcagg cctttttgca tcagcattcg aaattcgcgt ctgcaagttc agtgaaaagt
     6061 acgctctact cccgtctcta taaccttatc gaccgtgaca tggttgaccg tgaaggcatg
     6121 agctatcgca tcaccgatac cgggcgcgcc tggcttggaa aagcgttgcc ggcacgaaaa
     6181 agcgatccac gtaaagagct gctggaagcg gtgaaacgct acaacgagca gcaaaaagag
     6241 ctgctgcgcg aacaaatcag cacgatgaac ccgtacaaat ttgaacaact ggttggtcaa
     6301 ttactggaag caatgggcta cgagcaggtc gaagtgacca aagcctctgg cgacaaaggt
     6361 gtggacgtga ttggccaggt gcaggtcggc atcaccacta ttaccgaagt ggtgcaggtc
     6421 aaaagaatgc agaacactat cactcgccca tatatcgacc agttgcgcgg ggcgttgcct
     6481 taccacaaag ccattcgcgg tacactcatc accaccggga aatttgcggc gaaatgtgca
     6541 gaagcggcgc tctttccggg tgccgcgccc attacgctta tcgacggcga tcgtttactg
     6601 gaactgttaa tcgaaaataa cgtcggcatt cgccgcagca atgcagtgga attgctggat
     6661 gtggatttgc agttgtttga tgagctggag atggattaaa gggcgaaatc cgccgcctct
     6721 accattacaa tagcgaccca atttatctga acagagttca tcatggccaa agcaccaact
     6781 aaaaaagcca aagcaaagaa aggctttgaa gaaacattat gggatacagc aaaccagctt
     6841 cgcggtagcg ttgagtcctc cgaatacaag cacgtggtgt tgagcctcgt gttcctgaaa
     6901 ttcatcagcg ataagtttga agcacgccgc aaaaaaatga ttgccgatgg gcaagcagac
     6961 ttccttgaga tggaagtgtt ctaccagcag gacaacattt tctacctgcc ggaagaagcg
     7021 cgctggtctt ttatcaaaca aaatgccaaa caggacgata ttgcggtccg tattgacacc
     7081 gccctctcga ccatagagaa acgtaaccca acgctgaaag gcgcgctgcc agacaactac
     7141 ttcagccgtc agaatctgga aaccaaaaaa ctggcgtcac tgattgatac catcgacaac
     7201 atcgaaacgc tggcacacga aactgacgtt gaaacgttat cgaaagaaga cctggtcgga
     7261 cgcgtttacg aatacttcct cggtaagttt gccgcaactg aaggcaaagg cggtggtgag
     7321 ttctacacgc ccaaatgtgt ggttacgctg ttaactgaaa tgctcgaacc cttccagggc
     7381 aaaatttatg acccgtgctg cggctcggca gggatgttcg tgcagtcggt gaagtttgtc
     7441 gagagccatc agggtaaaag tcgtgatatc gcgctgtatg gtcaggaact gacagccacg
     7501 acgtataaac tggcaaaaat gaacctcgcc attcgcggtc tttcagctaa cctcggcgaa
     7561 cgaccggcag acaccttctt tagcgaccag cacccggacc tgaaagccga ctacattctg
     7621 gcgaacccgc ccttcaacct gaaagactgg cgtaatgaag ccgaattaac caaagaccca
     7681 cgttttgctg ggtatcgtat gccgccaacc ggtaacgcca actacggctg gattttgcat
     7741 atgctctcca agctgtcggc taacggcaca gcaggttttg tgctggcaaa cggttcgatg
     7801 agttctaaca ccagcggtga aggcgagatc cgtgcacaga tgatcgaaaa tgatctgatt
     7861 gactgtatga tcgccctacc tggacagttg ttctacacca cgcagatccc ggtgtgttta
     7921 tggtttatga ccaaatcgaa ggctgccgat ccggccaaag gttatcgtga tcgtcagggc
     7981 gagacgctgt ttattgatgc gcgtaacctc ggcaccatga ttagccgcac aactaaagag
     8041 ttaacggcgg aagatattgc caccatcgcc gatacctacc atgcatggcg cagtacgccg
     8101 gaagaactgg ctgcacggat tgcgcgtggt gacagcaagc tggaaaaata tgaagaccag
     8161 gctggcttct gcaaagttgc gaccctgcaa gacattaaag ataacgacta cgttctgaca
     8221 ccgggccgct atgtaggtgc agccgagcag gaagaggacg gcgtggcatt tgagacgaaa
     8281 atgcgcgaat tgtcgaagac gttgtttgag cagatgaagc aggcggaaga actggatcgt
     8341 gcgattcgcc agaatctgga ggtgctgggt tatggggagt aagtggatag aagtatcact
     8401 gggcgagatt tctgaaaaaa ttggagatgg gatccacgga acgccaactt ataacaattc
     8461 aggtaactat tactttatta atggaagtaa tttaattgat aattcaataa agataactga
     8521 gacaactaaa tgtgttgatc atgatgagta tttaaaacac cgtaagaaat tgagtaataa
     8581 taccgtttta gtttcaataa atggaaccat cgggaatact gcattatata ataatgaaaa
     8641 cataattttg ggtaaaagtg catgttatat caatctaaaa gataatatta gcaaacactt
     8701 tatactatat gtactttctg gttatttatt tcaagagtat atccaaaggt gttcaactgg
     8761 gagtacaatt aaaaacgtat cactaaaaat gatgcgtgat ttcaggtttt taatgcctga
     8821 atcaaaagag gagcaggaaa aggttgttcg tataatacaa aaaattgatg aactaaaaag
     8881 attaaataat gctcaaaatc aaaccctgga acaaatgttc caagccctgt tcaaatcctg
     8941 gtttgtagat tttgatccgg taattgataa cgctctggat gcaggaaatc caatcccgga
     9001 aaccctgcaa tctcgtgccg agttgcgtca aaatgtacgt aatagtacag atttcaaacc
     9061 gcttccggcg gaaattcgct cgcttttccc aagtgaattt gaagaaacgg agttgggttg
     9121 ggtgccaaag ggttgggaaa gtgaaacttt cgattcattc tgcgacttaa ttcagagtgg
     9181 gggaacgcct tcaaggaagg aaacttcatt ctgggatggc ggtacaataa aatggctttc
     9241 atctggcgaa gtgaaaggaa aaatagtctt agatacaaaa gaaaaaatca cagatactgg
     9301 attactaaat agttcatcta aattatggga aaaatataca accgttgttg caatgtacgg
     9361 tgcgactgcg ggtgaagtgt gtataattgg tgataaaatg gcggccaatc aagcatgttg
     9421 tggattgtat tcaaagacat tcccattttt tgtctataat tttgtctgca ataaagcaaa
     9481 tgaattagca tcaaaagcta caggttcagc gcaacagaac ctcaataaat taat
//
LOCUS       DAZKJR010000178         9459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKJR010000178 DAZKJR010000000
VERSION     DAZKJR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.179"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2859
                     /gene="evgS"
                     /locus_tag="ACHOWS_004554"
     CDS             <1..2859
                     /gene="evgS"
                     /locus_tag="ACHOWS_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFQ0050027.1"
                     /translation="RKDSIVLNEVLNRFVDALTNEVRYEVSQNWLDTGNLAFLNKPLE
                     LTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNIITLQTGLNFSPIT
                     VSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVFVMQKAPDSEQTLK
                     KGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELDALVATQLNSRYMI
                     DHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKM
                     PNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRK
                     ALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENSESPFKDVFSNTHE
                     VTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYICGWQDITETRDLI
                     HALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISL
                     AYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALS
                     CSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLGHIDDNHAVIKMTI
                     MDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIG
                     TIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDV
                     DEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSSLPIWGLTANAQAN
                     EREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQL
                     MQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLE
                     ITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(2915..4060)
                     /gene="yfdE"
                     /locus_tag="ACHOWS_004555"
     CDS             complement(2915..4060)
                     /gene="yfdE"
                     /locus_tag="ACHOWS_004555"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFQ0050028.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4134..5078)
                     /gene="yfdV"
                     /locus_tag="ACHOWS_004556"
     CDS             complement(4134..5078)
                     /gene="yfdV"
                     /locus_tag="ACHOWS_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFQ0050029.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5148..6842)
                     /gene="oxc"
                     /locus_tag="ACHOWS_004557"
     CDS             complement(5148..6842)
                     /gene="oxc"
                     /locus_tag="ACHOWS_004557"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFQ0050030.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNGIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6896..8146)
                     /gene="frc"
                     /locus_tag="ACHOWS_004558"
     CDS             complement(6896..8146)
                     /gene="frc"
                     /locus_tag="ACHOWS_004558"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFQ0050031.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(8659..9291)
                     /locus_tag="ACHOWS_004559"
     CDS             complement(8659..9291)
                     /locus_tag="ACHOWS_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFQ0050032.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         2714 a         2187 c         1977 g         2581 t
ORIGIN      
        1 agaaaagatt ctatcgttct taatgaagta ctcaatcgat ttgttgatgc tttaacaaat
       61 gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaatctggc ctttctgaac
      121 aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc cgatttaaag
      181 gtgctagaaa atccttactc gccaccctat tctatgacag atgaaactgg ctcagttcgg
      241 ggcgtgatgg gtgatattct taatattatt accttgcaaa caggtttaaa tttttctccg
      301 atcaccgttt cacacaatat tcatgctggg acacagctta accccggtgg atgggatata
      361 ttgcccgccg ctatttatag tgaagatcga gaaaataatg tttcatttgc tgaagccttc
      421 ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa
      481 aaaggaatga aagttgccat tccctattat tatgagcttc attcgcaatt aaaagagatg
      541 tatccggagg tagagtggat aaaagtcgat aacgccagcg ctgcatttca caaggttaaa
      601 gaaggtgaac ttgatgctct ggtcgcgaca cagttaaatt cgcgttacat gatcgaccat
      661 tactatccta atgaacttta tcattttctt attcccggcg ttcagaatgc atcactttcg
      721 ttcgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc actgaatgca
      781 attcccccaa gcgaagttct gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc
      841 attgacacat gggacctcta tagcgagcaa ttttatattg ttacgacatt atccgtttta
      901 ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg tcgtcgtaaa
      961 gttattcagg gtgatttaga aaaccaaata tcattccgga aagcgctctc ggattcctta
     1021 ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca ttagtcacaa tagtgcattt
     1081 gaacattatt tcactgctga ttactataaa aatgcaatgt taccattaga aaacagtgaa
     1141 tcccccttta aagatgtttt ttctaatacg catgaagtca cagcagaaac gaaagaaaac
     1201 cgaacaatat acacacaggt atttgaaatt gataatggca tcgagaaaag atgcattaat
     1261 cactggcata ccttatgtaa tctgccagca agcgaacatg ctgtttatat ttgtggttgg
     1321 caagatatta ccgagacgcg tgatttaatt catgcactcg aagtagagag aaataaagcg
     1381 atcaatgcaa ctgtcgcaaa aagccagttt ctggcaacaa tgagtcacga aataagaaca
     1441 ccaataagct ccattatggg cttcctggaa cttctgtcgg gttctggtct tagcaaggaa
     1501 caacgggtgg aggcgatttc acttgcctac gccaccggac aatcactcct cggcttaatt
     1561 ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact atcaacttca accacaatgg
     1621 gtcgatatcc ctactttagt ccagaacact tgtcactctt tcggtgcgat tgctgcaagc
     1681 aaatcgatcg cattaagttg cagcagtacg cttcctgaac gctacctggt taagatcgac
     1741 cctcaggcgt ttaagcaggt cttatctaat ttactgagta atgctctcaa atttactacc
     1801 gagggggcag taaaaattac gacctccctg ggtcacattg atgacaacca cgctgttatc
     1861 aaaatgacga ttatggattc tggaagtgga ttatcgcagg aagaacaaca acaactgttt
     1921 aaacgctata gccaaacaag tgcaggtcgt cagcaaacag gttctggttt aggcttaatg
     1981 atctgcaaag aattaattaa aaacatgcag ggcgatttgt cattagaaag tcatccaggc
     2041 ataggaacga tatttacgat cacaatcccg gtagaaatta cccaacaagt ggcggctgtc
     2101 gaggcaaaag cagaacaacc tataacacta cctgaaaagt tgagcatatt aatcgcggat
     2161 gatcatccga ccaacaggct attactcaaa cgccagctaa atctattagg atatgatgtt
     2221 gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg
     2281 cttattactg acgttaatat gccgaatatg gatggttttg agttgactcg caaactccga
     2341 gagcaaaatt cttccttacc catctggggg cttacagcca acgcacaggc taacgaacgt
     2401 gaaaaagggt taaattgcgg catgaactta tgtttgttca aaccgttgac tctggatgta
     2461 ctgaaaacac atttaagtca gttacaccag gttgcgcata ttgcacctca gtatcgccac
     2521 cttgatatcg aggccctgaa gaataatacg gcgaatgatc tacaactgat gcaggagatt
     2581 ctcatgactt tccagcatga aacgcataaa gatctacccg ctgcgtttca tgcactagaa
     2641 gctggcgata acagaacttt ccatcagtgt attcatcgca tccacggtgc ggctaacatc
     2701 ctgaatttgc aaaagttgat taatattagc caccagttag aaataacacc cgtttcagat
     2761 gacagtaagc ctgaaattct tcagttgctg aactctgtaa aagaacacat tgcagagctg
     2821 gaccaggaga ttactgtttt ctgtcagcaa aatgactaaa tagcggctcc cacaatgttc
     2881 aaatgtggga gctatttacc agcacatctt acatttatga tgagaactcc tggcgaattt
     2941 gttccccatg ctggtcgagc gttgccgctc ccggcataac atgcgggtcc gcgcagccgc
     3001 tgatttttat cggattcccc ggcatcatta ttcccccggc ttcaatcaac atatttctcg
     3061 cctgagtttg tggcaaatta atggcctcag ccacacttaa taacggcgcg acgggtacac
     3121 caacttcatg tattctggct aaccaaactt cagctgcctg cgtttttaac gtccgctcaa
     3181 tatattgttt aagaatagcc tggttttgta cgcgtaaaat attgctgcta aatctgggat
     3241 cattaaccag ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc ttgtcattac
     3301 cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg
     3361 gatggcgatt tcccaggcgt tgtggtgact tccctgtcgc aatatatgcc atcagaccat
     3421 gctccagaaa actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc cctctttggc
     3481 tcttttcgcg gccataaagt gcactcacta ttccgctgaa taaatagaca ccgccgcata
     3541 gatccgcaag agaggtaccc acgcgcactg gcggagcatc agggtatcct gtttccatca
     3601 ttatcccgct cattgcctga atgatggtat cgtaggcagg agcatctttt agcggaccgg
     3661 tatgtccgaa acctgacgat gaagcatata taaggcgcgg gttgatttct tgtaaccttt
     3721 cccatgaaaa cccgagtttt tccattgtac ctgggcgaaa attctcagct aatacatccg
     3781 cttgtttaag catatttata aatatacttt tatcgtgatc attctttaaa tcaagaacca
     3841 cactctcttt gccatgatta ataaaactgt aatagagtga ctgtccatcc acatagggac
     3901 caaatgtgcg ggtatcatca ccatgacccg gcggctcaac tttaattacc cttgcgccca
     3961 tattacaaag aagttgagtt ccgaaaggcc cattaaggac atgcgtcata tcgataacta
     4021 ataagccttc aaacggccct ttgcattcat tatttgtcat ttgcttatgc cttgaaaaaa
     4081 gccgaaggtg aaaagacact tcggcattta tgtaaaatca ataacatgat taattatgaa
     4141 accagtcgac tgacataaat ccacaaagga gccgtgacga caaaacccag tacgctcaca
     4201 gccaatgacg ctgtaccagt gcgggtgtag acattaaacc ggctggcaat aatgattccg
     4261 gagaatgccg gcggtaaggc gcctgccagt accatcatct gcaaatgttc gctgttcaaa
     4321 tgacatgcca taccaacgag aagcagcgcc agtggcatca gaatcagctt caggaaggtg
     4381 ttataagcaa tttcggcact gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg
     4441 aataccgcta cccctgagtt agccttcgca atcagattaa aggttgggtc ccatgctgcc
     4501 ggaattttta ccccaaccaa caccaggatc gttgccagaa caggtgccca tactactggc
     4561 tcctttgccg cagaaattaa tgcgctcaga ttactattct tcttaccatc cgctcctgag
     4621 gaaggattca gcaaatacag accaatagga atagtaattg cattaacaat aatagaaata
     4681 attgctacca ctaaacctgt tgatacggaa tcaccataaa taggatcgag aactgcaaat
     4741 cccaggaatc caatggtagg tgaacctgca attaatgcac atactgctgc ttctgcatga
     4801 gtacgtttaa aaaatttgta gcaaccgaac caggagaaaa agaaacatcc gacaataacc
     4861 acaagtgata caagggtcag acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta
     4921 atagatacaa atagagctgc aggaagagcg tagtttaata ccagtttatt aaatgcccgg
     4981 gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag catgattacg
     5041 ataataggca ataaatcgcc aataaaaaat gttagcataa tagtatttcc gataagggtc
     5101 ccctcaccat cgcataatga gttggcaaga ggagacgatg tcagtattta attaccagcg
     5161 acttgttttg ggttaagttt ggtaatatgg ccactttcag ttcctgctgc agggtcgatg
     5221 accacattaa taatggtcgg tttgcgcgac tgaataccgg tggttaaagc atgacgaagt
     5281 tcatctgtcg tagtgacgtt atagccaacg ccacgaaacg catccattaa tttgtcatac
     5341 cttgcatggt gcagcagatc cgttggtgat ggtgcaccag cgccgctgag atcaacaccg
     5401 tctcctctgt agatgccgcc attattaaaa ataacgatcg tcaccggcag gttatatcga
     5461 caaatcgttt caatttccat cccactgaaa ccaaaagcac tatcaccttc aatggcaaca
     5521 accggagaac cagaggtcac gctagcaccg atggcatagc ccataccgat gcccatgaca
     5581 ccccaggtgc cacaatccag acgacgacgt ggtttataca tatcaataat atttcgtgca
     5641 ttatccaggg tatttgcccc ttcattaact aaataaatat cctggttctc gcgcaatacg
     5701 tcgcgcacag cacttaatgc attaaagtaa tttaatggtt gagtatctgt gcttaacttt
     5761 tcatgcattt tttgtgcatt ttgctgcttg tggatattta aaatatcgcg ccataccagt
     5821 ggagtcgtaa atgtgttttg tttcagttct gccagcatac cttgcatact ggatgcaata
     5881 tcgccaacga ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc
     5941 agttgaataa actgtgtatc tgccgcccat ccttttttac cgtgtgccag taaccaattc
     6001 agtcgtgcac caacaagcat gacaacgtca gcatttgcca gggcaaacga acgcgcagcc
     6061 gccgcagaaa gtggatgcgt atcttcgagg atccctttcg ccatagacat tggcaggaat
     6121 ggaatctggg cactttcaat aaattcacga agctgttcat ccgcttgtga atacgccgcg
     6181 cctttgccaa ggataactaa tggtcgttca gcttttgcta atagcgaaat tgcgctagcg
     6241 actgacttcg ggcatggcaa taatgctggc gacggatttt caacttttac aatcgtggtt
     6301 aacgcttcgt ctttttccat cgtcgcggcc aggacatttg ctggcaaatc aagataaact
     6361 ccgccagggc gacccgatac tgagacccgg atagcgcgtg ccaatgcaat gccaagatcc
     6421 tgcggctgat taacgcggaa tgctgctttg gcatacggtt ttgccgcatt catttggtcc
     6481 agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc
     6541 atgatcatcg gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc attgaggaat
     6601 cctggcgcag aaactgtcag gcagatcccc ggtttttggg taagaaaacc gcttgccgca
     6661 gcggcatagc ctgccgactg ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg
     6721 gcatggcgcg ccatatccgt cacaggaata cctacaacac cataaatagt gtcaatacca
     6781 ttctgtttta atgcttcaac gatgatatgc ataccatctg tcatttgaag ttgatctgac
     6841 atgattaata acctcgaaat ttttataaat tgggagccag aagcccccgt taatatcaga
     6901 tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct ataacccagc tcctgcaata
     6961 cagcagcggt atgctcacct aatagcggcg cagctttaat atccggcgta aaggcagaga
     7021 atttcattgg acaaccaacg gtcagatatt ttccacgcaa cggttgttcc acttcgacaa
     7081 cactaccact ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt aaaaccggtg
     7141 cacaaggaat atcaaactga gtcaaatagg ccaccgcttc atgtttatca atagtgacag
     7201 tgtatttttc gatttcagca aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac
     7261 tgtatgccgg atcggtaatc cattctggtt tgccgatggc tttacaggtg ttttcccagt
     7321 tttgctcctg aatagtgaaa taaatatagg cgttaggatc ggtttcccag cctttacatt
     7381 tcaggatcca gccaggctga ccgccaccac ccgcattacc accgcgggga actgcatcac
     7441 caaatgtgcc attcggatac tgcgggtatt cttccagata ccccaactta tcgagacgct
     7501 gctggtcgcg taatttcacg cggcaaaggt tcaacacggc atcctgcatt gacatggtga
     7561 ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca
     7621 aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct taccagcggc ggaccgtccc
     7681 aaaaacctgt agtagatgcc gcgccacccg ctgcctgagc aacgttttca taggctttta
     7741 cattcacata aggcgaacac tcatcaaacc ctttgatcga accaaaaatc agacgtggat
     7801 tgatttcttg aatatgctcc caggtgaagc ccatatgatc aatggcccct ggatgaaagt
     7861 tctcgactaa gatatcagct tcgcggatca gcttttccat tacctctttg ccttccgctg
     7921 ttttggtatt taattcaata gaacgtttgt tactgttaag catggtgaag taaagcgcat
     7981 cgatatcagg aatatctcgc agctggtggc gcgttacgtc accaacgccg ggacgttcaa
     8041 ttttaatgac gtcagcgcca aaccaggcca gcatctgagt acaagatggg ccagattgca
     8101 caccagtgaa atcgagaact ttaattcctt gaagtggagt tgacatagca atacctatta
     8161 gttaattaaa tatatgttaa tttagcgttt atttaaggca taatgctctc tataaataat
     8221 ccgcaaaata tcgtcgtagc tgcactcgat atttttaaat tcctgtatca agtgctgata
     8281 atcatttcat cgccacttcc aattcctttc tgcatggtaa gtagtgagga cgaaaagaag
     8341 aatatgataa atattggttc gcggtaatca gagagggccg cgacatttgt gatgttattc
     8401 ttacaactgt aagaagcgcc ggtgatgtac aagtaagggt atgattacgc tttttaaagc
     8461 gtaccataaa acataacata ataaatgatt tatgatgaca cgggattatt gcggtttact
     8521 ttgaagaata taaaatgcga gtttatagca ccgcgagcgc caaataattt tatggccaca
     8581 acctgtcgta ttgtggccat aaaaaattgt cggctggagg gagtaaaatc ctttacctca
     8641 atatattgcc tgtcagcttt agttcacaaa cagtgcttcg ctatcgacga tgattccatc
     8701 ttccgcacct ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa gcagtttctg
     8761 cgcatcagca agtgcattac gagtctgttt gagcggcatc agatattggt tttccattac
     8821 accaacaccg gccaggcgaa gctcttccat tgcgcccttt ttatcccctt tcgccatttt
     8881 ttcgttggca atttttatcg cggcttcttt ttcgggtgtc gctacataac tctcagaaat
     8941 tccgacagat gcattaatga caatatattg gtcatcattg agattggtct ttttacctgg
     9001 cttagcgaac tttgcccatt cggtactgtc atctgacaac aaagctgaag cttcattggt
     9061 cagttctttt gccttttccg gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc
     9121 acgcatcgca tataaacctt gttcagagat acgctctgct gctgccgttt tctgggtttg
     9181 ctgaacttgc tgagtttgct gttgtgccgc taccggtgca ttatcagcag cccatacagt
     9241 tgaagatgcc agaattgctg tgaccatcgt agccataatt aaacgtttca tacttacctc
     9301 attctttaac agttttagaa aacgccgcca tcatattggg tgagcatgaa atgagaaatc
     9361 agagagcaac ggtcattttg aaagggttat cttacagttg tagttattat cgcaagaagt
     9421 attttagtct tacaattgta ggaatatgct tcctgtcac
//
LOCUS       DAZKJR010000179         9356 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKJR010000179 DAZKJR010000000
VERSION     DAZKJR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.180"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..1155
                     /locus_tag="ACHOWS_004560"
     CDS             31..1155
                     /locus_tag="ACHOWS_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFQ0050033.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     SPGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            1136..3439
                     /gene="trbC"
                     /locus_tag="ACHOWS_004561"
     CDS             1136..3439
                     /gene="trbC"
                     /locus_tag="ACHOWS_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFQ0050034.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEVIQSGDKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            3906..4136
                     /locus_tag="ACHOWS_004562"
     CDS             3906..4136
                     /locus_tag="ACHOWS_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050035.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(4234..4665)
                     /locus_tag="ACHOWS_004563"
     CDS             complement(4234..4665)
                     /locus_tag="ACHOWS_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0050036.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(4733..7444)
                     /locus_tag="ACHOWS_004564"
     CDS             complement(4733..7444)
                     /locus_tag="ACHOWS_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0050037.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDMQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPVSGHINRLSWSQERLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYEP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(7456..7788)
                     /gene="mobA"
                     /locus_tag="ACHOWS_004565"
     CDS             complement(7456..7788)
                     /gene="mobA"
                     /locus_tag="ACHOWS_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFQ0050038.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            8021..8356
                     /locus_tag="ACHOWS_004566"
     CDS             8021..8356
                     /locus_tag="ACHOWS_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFQ0050039.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKRAPKKTDSGR
                     NDGIDMNNN"
     gene            complement(8441..9289)
                     /locus_tag="ACHOWS_004567"
     CDS             complement(8441..9289)
                     /locus_tag="ACHOWS_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFQ0050040.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT         2373 a         2599 c         2139 g         2245 t
ORIGIN      
        1 acagcatccc gccgacaagg aaaaagaaca atgaccatcc actacactgc aacactgaat
       61 aataaacacc tgtccctgac cgccctgtac cctgacggta atcgggaggt gattgccatc
      121 ctgccagtca cagaactggg cgcactgatt aaccgtaata acaggctgat gcacctgtcc
      181 ttcagttccc cttttcgcga aataaccgaa acggtgcttg ttgataccgt tgatgcagaa
      241 cgtcccggac atcacatcgg cagaatgctc tcagcgattg atgcctgcct ggatgccgaa
      301 tatgccggaa aaggaaaaca tccctcccgg aaaaatggct tctggcggac actgatgtgt
      361 ggctttatcg ccggaattgc tctgccggcg acagttgcag gttaccactg gatgaaggca
      421 gagccccctt ccacgctggc ttaccctaca tctcaggaat ggagagctga accagtaacc
      481 gacacagaga caccacgccc acatacggaa catcatgtta acagcatccc ggcccgcccg
      541 gctcccacac agcaggaacc ttcttctgcc cccgccccga cggcagtgac ggaagaacaa
      601 cacgttcagg ccgctgaggc ccgccgtaat ctggccacag tacttaaacg caacgcagac
      661 cgggggatgt tcaccattaa cctgtcccct ggtcatgaac gcacgctgta cgccttcctt
      721 gaccccgcat gcatcaactg ccgtctgctg gaacccgcgc ttaagcaact ggcccgggaa
      781 ttcagcgtgg tgatttatcc ggtctccgtg attggcgggg agtccagtac aaagcggact
      841 gctccgctgc tgtgtgaacc ggactcacaa aaacgggcga ccggatggca tcagctgtat
      901 tccgctgaca gtggtatggc ccccaccggc gatgcaccgg ccgcacagga tggacagtgt
      961 ctgaccgccg cacgtgctgc catcgatgtc aataaccagg ctttccgcca gttcggtttt
     1021 ggcggaaccc cctgggtgtt atccgatacc ggctggcacc tgccgtccgg catcctgaaa
     1081 gacgccactt ccgttcgcct cttcctgaaa gcaacagata gcgaggagta tcacaatgag
     1141 ccagcaccac gttaatcctg acctcataca ccgcacggcc tggggaaacc cgttgtggaa
     1201 tgcactgcat aacctgaaca tcactggtct gtgtctggcc ggcagtatca ttacggcgct
     1261 gatatggcca ctggcccttc ctgtctgcct gctgttcacc ctggtcacct ccgtgatatt
     1321 catgctgcaa cgctggcgat gccccctgcg tatgcccatg acactcagcc ttgatgaccc
     1381 ctcccaggac agaaaggtca ggagaagcct cttcagcttc tggcccaccc tgtttcagta
     1441 tgaagcggat gaaacattcc ccgcccgggg gattttttat gtcggttacc gccgtatcaa
     1501 cgatatcggt cgtgagctct ggctgagcat ggatgacctc acccggcatg tcatgttttt
     1561 tgccaccacc ggtggcggta aaacagaaac cacctttgcc tggctgctca acccgctctg
     1621 ctggggaaga ggctttacct ttgttgacgg caaggcacag aacgatacca cgaggacaat
     1681 ctggtatctc tcccgacgtt ttggcaggga ggatgatatc gaagtcatca acttcatgaa
     1741 cggcggaaaa tcccgcagtg aagtcattca gagtggcgat aaatcccgcc cacagtcaaa
     1801 cacctggaac ccgttcgcat tcagcaccga agccttcacc gccgaaacca tgcagtccat
     1861 gctgccccag aacgttcagg gcggtgaatg gcagtcacgg gcgatagcca tgaacaaggc
     1921 actggtcttt ggcacaaaat tctggtgcgt ccgggaaggt aaaaccatgt ccctgcagat
     1981 gttacgggaa catatgacgc tggaagggat ggcaaaactc tattgccgtg gccttgatga
     2041 ccagtggccg gaagaagcca tcgcaccact gcgaaactac ctgcaggatg tcccgggatt
     2101 cgatatgtca ctggtgagaa cgccgtcagc ctggactgag gagccccgta aacagcatgc
     2161 ttatctctcc ggtcagttct cggaaacgtt cacaaccttt gccgaaacct tcggggacgt
     2221 ctttgcggca gatgccggcg acatcgatat tcgcgacagt attcacagcg accgcattct
     2281 gattgtgatg atccctgcac tggacacatc ggctcatacc acgtcggcac tcggtcgtat
     2341 gttcgtcacc cagcagagca tgctcctcgc ccgtgacctg ggctaccggc tggaaggcac
     2401 agatgcacag accctggaag tcaaaaaata caaaggcagc ttcccctata tctgtatcct
     2461 tgacgaagtc ggtgcctact acacagaccg cattgctgtg gaggccaccc aggtacgttc
     2521 actggagttt tccctgataa tgacggcgca ggatcaggag cgaattgaag ggcagacctc
     2581 cgccaccaac accgccacac tgatgcagaa cgccggcacc aaattcgccg gacgcattgt
     2641 cagtgacgac aaaacggcaa gaacggtgaa aaatgccgcc ggtgaagagg cacgggccag
     2701 aatgggcagc ctgcagcgcc acgatggtgt gatgggtgag tcctgggtgg acggtaacca
     2761 gatcaccatc cagatggaaa gcaaaatcga cgtgcaggat ttgatcagac tgaatgctgg
     2821 tgagttcttt accgttttcc agggggatgt ggtgccgtca gcctctgtgt atattccgga
     2881 cagcgagaaa agttgcgaca gtgatccggt ggtcatcaac cgctatattt ccgttgaagc
     2941 cccccggctg gagcgtctcc gccgtctcgt ccccaggaca gtgcagcgca ggctccccac
     3001 accggagcat gtcagcagca tcatcggtgt actgacagca aaaccatccc gtaaacgtcg
     3061 taaaaaccgt acagagcctt accgtatcat tgatacattc cagcgccagt tggcaacagc
     3121 gcagacatca ctggatctgc ttccgcaata tgatacagat atcgaatccc gggcaaacga
     3181 actgtggaaa aaggctgtgc acactatcaa taacacaacc agggaagaac gccgggtatg
     3241 ttatatcaca ctgaatcgtc cggaagagga acactccggt cctgaagata taccttcagt
     3301 gaaggcgatt ctgaatactc tgctccccct ggagatgctg ttacctgtac cggatctaac
     3361 tgcatccccg ccccataaaa aaaatgtcgc tcagacacca tcaggtggaa aacaagagag
     3421 tcgtaaaaaa agattctgat aacacctgta tggctgattt taataacaac ctttacgcga
     3481 ttattaatct cctccgctca ccatagtaag cggaggattc aggatttcaa tccatcacca
     3541 gaggtacaat aaatgtgtat tagtagcccg gaaacaaact catggtctgt tatttatcgt
     3601 aaaaattccg gagaagatat caatatcact tccctgacgt ttaaaaatag tcttctggca
     3661 gcccggacac tgatggtgcc cgaaaactat atgatatgca tactcagaaa cggggagagg
     3721 gtcagacgat gggacagaga aatacttgca ggctcaaaca gatggtacaa gtgttctccc
     3781 gataatttcg aaatactggg taagctcccc ataataaata aagtcacaaa actgataaaa
     3841 tcataatcta cggattttaa tcagttaagc taaatcagga tgaacgcttt gattatgaat
     3901 tatacatgaa aacaaatatg ggcagcacaa aaaatggtgc cagggttccc cacgttcgta
     3961 atagattcac ttctatggat attggtatga attcccagat cctgccagac atgctgatta
     4021 attttgccct tattaacatc acagacagaa agaacgaggg taccaatacc attgatggta
     4081 actggcaggc cgatgaagga cgccgttacc gggataatgt aagaatctat ttttgagtat
     4141 gtcaacataa cctgaggcaa agtacctcag gttccagtgt agtaatcagc caacctgaca
     4201 gtcttctgtt agccctacag atatgtcgtg gagctaaccg cgcatgtcaa agccggattt
     4261 ttgaaaatct ctataaactt ccggattgtt gaaggccggg aatttagctt tatgggctgc
     4321 tgactttatc gcttcgcaat aggctttatc gccattactg gttgatattt ttaacgccgt
     4381 gccatcccgg gagaattcca tatgcaacct gcattttttc cttttccatt tttgcggatc
     4441 atcaagttta gcattaattg catccctgat tccccgtgct tgctcccccc attcatcctg
     4501 atcatcccaa cgtcctgaac tgcaactacc cagggctgta gttttatggc aatttgaagg
     4561 atgtaatggc gcacatcccg ccacaaagct gactaaaata gccagcataa cgattttctt
     4621 tacccccata tattgctcct caatccatat agatctcagt accatagctc tgtcagagca
     4681 aagaaaatct taactaatac aagttaagat ttttaatatc cactcctcat ccctacattc
     4741 caggtgatgg tggcgtccac tcattatcct gaactggagc ataagatgcg ccatgccctc
     4801 cggatatgct tttctgttgc ctgatatatt cgtggtagta ctgttcacta cgctgacgat
     4861 gtaaatccac atccgggcgg ctgattacaa agtgtccatt tccggttatt ccagctgcat
     4921 tatgccaggc caccatttcc gcaaatacat cattaaactg ttgatatgag ccttccggtt
     4981 caaaacccat tcctggctca cgatcaaaca atacaggagc aatcagattc agcttatcca
     5041 ccagttcatt tctgtcctga tgatggtaga acaccacacg gtccccataa tccacacaga
     5101 ctaactcgcc attcctccga tcacgaaaac gcacactgcc atctttctgc agacgggctt
     5161 cgagaacgcc agtgtcttca taaagtggag ttcctcccgg ttcacagaga atcacgcagc
     5221 ggtcggcatt cgttgttcgt cgcttgtctt tgcgacgatc acgataatcc cagccacgta
     5281 actgtgataa tgcagctctg tctccctgta ccgcctgttg ctccacccac tgccggtagg
     5341 acaaaggata ccacttaccc tccgcaatca gtgacaaccg ctcttccttc acactctcct
     5401 tcagcctgat cagcgcctgc atacgctgca cttctgcaat atggtaatgc agcttacgca
     5461 attgtggatc ccggaactgc acacggatat acgccttgcg ccgacggcag gcagcatgaa
     5521 tttcccgtaa ccgttctccg taacgtaagt cgggtttacg ccagtgctcc ttccatgcaa
     5581 gataacgcgc gcgcaaatct tcccgcgcag ctgcacgggc ttcacggcgt tcacgacgca
     5641 gtacaggatc gcgccggaag ccgggctcca cttcatcgct ggcagcaagt tccggattgt
     5701 agcgacattc aggcttcacc cgttcaaata tatctgcacc tgcaatctca aatggccctg
     5761 cctgcggctc agccctggat aacgtcaaat ccgggtggat actgctggcc ttcactggtg
     5821 tgagctcatg attaaacgca tccacaatca caagaccatg atgctgtttc tgcaacataa
     5881 gccctttacg cgcaaacatc tcatggcacg cctgccagct gaaattcccg cccgcgatca
     5941 tatcttcaat caacgactca cactgaacag tcgtgtaccc ccacagactt tccttatcag
     6001 aaaacagttc gcgctttatt gctccctgtg gacctgtccc catccgcata cgataccatt
     6061 cgtaatcact gacagcctga gtaccaatat tacgtccccg gaatcgttct ccgggtataa
     6121 cctgagtaaa aatatccttc ggtacaggct gccatccacc atccagatcc tgcatataat
     6181 ccagcgtcat taacggccac acactgtctg cacgtactgg tgtctgttgt ttatcaaatc
     6241 catcatgcag taccagatgc ccgtgctgga tccccatcca taatccagcc tttgccagag
     6301 ttctgtgcac atccaggcaa ctttccagct caccatttct cgccatttcc ggtaagcgtt
     6361 cagcaaagtg acgacaggcg tactgtttca gactctccgt ttctgccact ttttccggcc
     6421 ggatatccac gttaatggct tccggctcat aacgaccggg tgtccccacc tgactgaaaa
     6481 tatctgtcgg tacaggctga tattccccca gtttccgccc cagtttttca gctgtccatg
     6541 acggtccgaa cgaactgagt gcaacacctt cccgcgctct gtcccagcca tccttcacca
     6601 ccagttcccc ttcctgcatc gtgatataca gaccatcact ggccagccgt ttatgcaaag
     6661 ataaccagtc ctgtgcaggc tcttcacgca gcccggcaat cgacatctga gcaatatatt
     6721 ccctgaacgt ctgctttttc ccgcgtcgcc aggcattacg gcgctcacgt accagcgctg
     6781 ttttgcgcac aatacggttg ccgggtgcat gcacaaagca gccattatca ggcgcgaatc
     6841 cgtgcttaag ttcaagttca cgacatgcac ggctgagcct ttcctgactc caggaaagac
     6901 gattaatatg accactaaca ggatgaacac gattcactgc gacatgtaca tgcaggttat
     6961 ccgtgtccgt atgaacggca gccacgtact gatgttttga gagttcaagg gatttcaatg
     7021 tgtgacgcac acagtcaaaa agctgctcag gacgcgggct ttcatgcgca ggccaggaga
     7081 gaatgtagtg gaaaacgggg tccgtatcat tacgggaaaa aacagctttg tccgccttgt
     7141 actgcatctc ttcagcagct gtttccagtg aattgcagtt atggaaacag gtaacgccat
     7201 agaaatttac ccactgactg ccgtctttca tcacgtcaat cagggagaca aatttttcgt
     7261 tacgcaggcg tgtagcatag tcgacaaggc gtctgaaacg gttgcggtga gggacgtcac
     7321 tcttgatttc gctgtcttct gtgagctcat tttcctgcat atcatcccgc acagatgtgt
     7381 acgcaatcag gtcttcaaac gaagacctgc cgtcacgtct tttgtgcgga ataatggcat
     7441 tcatcagcag ttatctcagc gtcccattaa gtctcttcgc agagcattga catattcagt
     7501 aatggcaaca agaacctcgg catactcctt atcaccagta cgttttcctt ccacaaacag
     7561 atgcttctga aggcggccaa gacgcataat ctcatcaatc atacggtcgt cagtacggca
     7621 gttaaccacc ttatccagtg aagagcttcg tataaatgtt gaaaccgtct gtccacttga
     7681 ctccgctttt tctctgacga tttcagcctc atcctccgtg aatctcgccg tgaattttac
     7741 aattctcttc ctgttctcac tgcccgttcg ggttttcttc tcactcatgg tctgctcctt
     7801 accggggtgt cggggcgaag ccctgaccag gaggcatttg tctgattgcg cgtgcgcggt
     7861 ccgacaaatg cacatcctgt cccgtttttc aggacattat agcctgttat ttttgtgcat
     7921 catagtctta tgcagacggc agaggcaggg cgcagccctg ccaggagtca gtttttgatg
     7981 ataaaatcag gcttcaacat gaaacagaga gaaagtgcca atggcttcta aaaaatttta
     8041 ctcagatgat gacatccagc tggcaaaagc tgctttatcg gaattgccag atctcaccgc
     8101 tcaacggaaa acattgcgtg attttcttga tgccattcgt gatgacatta ttgtcctggt
     8161 aaggacaaag ggttatacac tggcagatgt tcgtgacacg ctcaaaaatg cagggtatga
     8221 agtaggagag aaggcacttc gtgacattat tcgggaagca gaatcgaaaa aaccatcccg
     8281 ccgttcttcc tctaaaagag cacctaaaaa gacagattca ggcaggaatg atggcatcga
     8341 tatgaacaac aattgatgtg acaatgtcac atcgtgttct gagattatga tgattacaga
     8401 aaaaaagccc cggcataaat accagggctt ttctgtttaa tcagactcgt ggcggcaatg
     8461 caccaggata atgccgtgca ataatatcat tcagtttttc aacaaggtca ggacgcttaa
     8521 agacaatgtg tacggtccct ttctggtagc cacgcaccgt aaaatattcc ccttcaaaca
     8581 actcaccgac attgtcgccg ttcccgagaa attcaccata ctgcgccccg atgctgttcc
     8641 tgaagtcagg aacgttgcgc ccctccagca gataaaacgg acgtaccagg tcatccagtg
     8701 cctcccgtcc gctgcagtcc agcgtcacac gcccgttact ccagcggtac agcaggtttt
     8761 caataatgat tttctttccc agacggcagg gattattggt tctgtaatcc catgacaatt
     8821 tccggtagac atcaatcagt ccatgttcaa atgtctgcat cttactggca ttcagatggc
     8881 gaaaagttgc cagtacattg tccagggtga tttcaggaca ggtgtcgctc atcagctggc
     8941 tgttccactc atcacgctgg catgaactca tgaatgtgta catgccagta tccgtcatca
     9001 ggcgatccca gattttccgg tcggcaagac gggtgatatt cttttttgct tcttccggtt
     9061 tatccatgca tcgaacggag tcagtcacgc agtcttcaaa cccgtagaga aaaatgtcac
     9121 ctgacgcatc aagcagaatc ctgcgtgctg tcgccaggca ttccatcgcc tgcatgaaca
     9181 gtgaaatacc ttcgttacgc tgcgcgatga tgcggtcgat agccacagac ggaataacat
     9241 ctgtcgtgcc gtcagtattc aggtcagcag tgggtaaagt ctgattcatc acattgtctc
     9301 ctcatcgtca cgactgccgg gtcagccagg tcatttcccg gaggagcgaa gctcct
//
LOCUS       DAZKJR010000180         9275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKJR010000180 DAZKJR010000000
VERSION     DAZKJR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.181"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..1777
                     /gene="adeD"
                     /locus_tag="ACHOWS_004568"
     CDS             11..1777
                     /gene="adeD"
                     /locus_tag="ACHOWS_004568"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0050041.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNTYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTISRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVIRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1824..3215)
                     /gene="uhpT"
                     /locus_tag="ACHOWS_004569"
     CDS             complement(1824..3215)
                     /gene="uhpT"
                     /locus_tag="ACHOWS_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFQ0050042.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQSFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3471..4790)
                     /gene="uhpC"
                     /locus_tag="ACHOWS_004570"
     CDS             complement(3471..4790)
                     /gene="uhpC"
                     /locus_tag="ACHOWS_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFQ0050043.1"
                     /translation="MLPFLKAPADAPLLTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGVSALLLLPFLNAQTPREA"
     gene            complement(4800..6302)
                     /gene="uhpB"
                     /locus_tag="ACHOWS_004571"
     CDS             complement(4800..6302)
                     /gene="uhpB"
                     /locus_tag="ACHOWS_004571"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFQ0050044.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6302..6892)
                     /gene="uhpA"
                     /locus_tag="ACHOWS_004572"
     CDS             complement(6302..6892)
                     /gene="uhpA"
                     /locus_tag="ACHOWS_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFQ0050045.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6965..7255)
                     /gene="ilvN"
                     /locus_tag="ACHOWS_004573"
     CDS             complement(6965..7255)
                     /gene="ilvN"
                     /locus_tag="ACHOWS_004573"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFQ0050046.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7259..8947)
                     /gene="ilvB"
                     /locus_tag="ACHOWS_004574"
     CDS             complement(7259..8947)
                     /gene="ilvB"
                     /locus_tag="ACHOWS_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFQ0050047.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAIAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDSAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9053..9151)
                     /gene="ivbL"
                     /locus_tag="ACHOWS_004575"
     CDS             complement(9053..9151)
                     /gene="ivbL"
                     /locus_tag="ACHOWS_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFQ0050048.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
BASE COUNT         2248 a         2656 c         2428 g         1943 t
ORIGIN      
        1 tgagaaaaat atgaataatt ctattaacca taaatttcat cacattagcc gggctgaata
       61 ccaggaattg ttagccgttt cccgtggaga cgctgttgcc gattatatta ttgataatgt
      121 ctctattctc gacctgatca atggcggaga aatttccggc ccaattgtga ttaaaggacg
      181 ttacattgcc ggtgttggcg cagaatacgc tgatgctccg gctttgcagc ggattgatgc
      241 ccgcggcgca acggcggtgc cagggtttat tgatgcgcac ctgcatattg aatccagcat
      301 gatgacgccg gtcacctttg aaaccgctac cctgccgcgc ggcctgacga ccgttatttg
      361 cgaccctcat gaaatcgtca acgtgatggg cgaagccggt ttcgcctggt ttgcccgctg
      421 tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtac ccgccctgga
      481 aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct ggcgggacca
      541 tccgcaggtt accggccttg cagaaatgat ggactatcct ggcgtaatta gcgggcagaa
      601 tgcgctgctc gataaactgg acgcatttcg ccacctgacg ctggacggtc actgcccggg
      661 tttgggtggt aaagaactta acacctatat tgctgcgggt attgaaaact gccacgaaag
      721 ttatcagctg gaagaaggac gccggaaatt acaactcggt atgtcgttga tgatccgcga
      781 agggtccgca gcccgcaatc tcaacgcgct ggcaccgttg atcaacgaat ttaacagccc
      841 gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggag atcgcccatg aaggacatat
      901 cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg tggcatatcg
      961 cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct tactggcacc
     1021 cggtaagcag gccgatatcg tcctgttgag cgatgcgcgt aaggtcacgg tgcaacaggt
     1081 actggtgaaa ggcgagccga ttgatgcgca aaccttacag gcggaagagt cggcgagact
     1141 ggcacaatcc gccccgccat atggcaatac catttcccgc cagccagttt ccgccagcga
     1201 ctttgccctg caatttaccc ccggaaaacg ctatcgcgtt attgaggtca tccataacga
     1261 attgattacc cactcccgct ccagcgtcta cagcgaaaat ggttttgatc gcgatgatgt
     1321 gtgctttatt gccgtacttg agcgttacgg gcaacggctg gctccggcct gtggtttgct
     1381 cggcggcttt ggcctgaatg aaggtgcgct ggcggcgacg gtcagccatg acagccataa
     1441 tattgtggtg atcggtcgta gcgcagaaga gatggcgctg gcggtcaatc aggtgattca
     1501 ggatggcggc gggctgtgcg tgatccgtaa cggtcaggta caaagtcatc taccgttgcc
     1561 cattgccggg ctaatgagca ccgacacggc gcagtcactg gcggagcaaa ttgacgcctt
     1621 gaaagccgcc gcccgtgaat gcggtccgtt accggatgag ccgtttattc agatggcgtt
     1681 tctttccctg ccagtgatcc ccgcgctgaa actaaccagt caggggctgt ttgatggcga
     1741 gaagtttgcc ttcactacac tggaagtcac ggaataataa aaaaaagccc ggcgtcatgc
     1801 cgggcaaaag tcaccagtta agtttatgcc actgtcaact gctgaatttt tttctcgcgg
     1861 cggattttgc gttcttccat caccgccact atcgccatca ggcagataca accaatcgcg
     1921 gcgatatcca gcgcggcgaa ggtgcctgcc cagccggtaa ggccaaatac cggcgtccca
     1981 tcggcaatca ttcccagacc taacttggca aagctgtcac caatcaggta agcgaaggtg
     2041 cctttaatac catcggcagc gccaatcgct tttttaggta caaagccaac agcagccaca
     2101 ccaatcaaca attgcgggcc aaagaccagg aaacccaacg caaagagaga agccaggtag
     2161 atatattcgt tgctggcgtg ttgatacaca ccgagcgtgg cgataatcag cgccagcgcg
     2221 atacaggcca ccaggccacg gcgaccgttc gccaggtcag agagccagcc ccacagcagc
     2281 gtaccgacca gcgcaccagc ttcaaacagc gtaaagctct gaatcgccac cgctttagag
     2341 agtttcagtt cctggaacgc atacacggtt gaccactggt cgataccaat acgcaccaca
     2401 tagaggaaaa tgttggcgaa gcacagcagc cagatcactt tgtttttcag cacatactca
     2461 acaaagatct gccacttggt catatcggta gattctgtct ctttgtcctc ttcgctgatc
     2521 tcctcgccga acagctcttc agctttgccg aggccataag attccgggga gtcgctgccg
     2581 taacgcaggc cgataaaacc gacaatcagc gcgataatcg acgggaaaat aaacatgccg
     2641 atgacatgac catcgaacag gtaatttgcg ccgaacagcg ccacacctgc tgcgcctgca
     2701 ccaccaaggt tgtgagaaat gttccagaaa ccgaggaatg tcccgcgttt acgacgcggc
     2761 gtccatttgg tgatggtgga gtaactgcac gaaccgccgg tactctggaa aaagccgctc
     2821 aaggcgtaga aggcaatcat caggaacagg ctaaccgagc cgctgcccat actggcactg
     2881 aagcccagca tacaaatggc ggacaggatc agcatgaacg gcaggaattg tttggtgttt
     2941 ttgccgtcgg cgtagtaaga aaccagcgtt ttacccacgc cataagtgat ggagaagccc
     3001 aggccgatca tccccagctg cgtcatgctc aacccgtagg tcgagatcat gtcgttctgc
     3061 gcgatgttaa agttcttgcg aatcaggtac atcgtaaggt agccgataaa gaccaccagg
     3121 taggattgca tgaacggttt gaaccacatt ttgcgccgca cttcgagcgg aaggtccagg
     3181 gtcggcttgc gaacctggtt taggaaagcc agcatgggtt actcctgatt tcccttcata
     3241 cttcaagccg caggtgcctt agctgcacgc attcctcccg gtcacatagt tcactatgct
     3301 cccgggactc acgcgtttgc tgcgttcctg caacttgaat tattttggga aagaattaat
     3361 gaatgatggc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc caggcaaaac
     3421 ctggaaaatt gcttagtttt gccggatatg aagtgaaaag gtgatatgca tcacgcttcg
     3481 cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaacccc ggcggcaatg
     3541 gcgataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc cagcggccaa
     3601 ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc cgcccctgcc
     3661 gcctctttgt gggaacattc tgccgccgcc atgccgatta acatctgcgg gccaaagaca
     3721 aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa tggcatcagc
     3781 cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat cggcccgcgg
     3841 ttgccgttaa acaatttgtc cgagccccaa cccgctacca gcgcaccgat aaatccaccc
     3901 agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag cgtctcggac
     3961 atatacaaat tgccccagtc gttgattgcc gcccggacca catagaccag cacatagcaa
     4021 aacgaaagca gccagatata cggattcagt aacacatatt tggtgaggat ctctttacgc
     4081 gtcagccctg ccccttcttg ttgttgagct atctccagcg cgtcgtgccg ccattcacca
     4141 accgccggta aacctaacgc ctgcgggcga tcgcgcagcc gccagcagag aaaaatcccc
     4201 acgactatcg ccatacaacc cgcaatcatc atcccggcac gccagccgta atgcagcgcg
     4261 ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt gttccataat
     4321 gcccaccaac cgccgcgctc ggtacgagaa taccaggcgg ttaacagacg cgcacacacc
     4381 ggtgaacccc agccctggaa aaaggcgttc aacacccaaa gcacggcaaa cgcccatagc
     4441 gacgtcgaga agccaaacag aatgttgata atgcccgtgg caataagccc tatccccata
     4501 aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga cacgccgtag
     4561 gtaatgtaaa acagggtcgc taacaggcca atatcgctac ggctgagcac gccgttagca
     4621 aggatttctg gtacggcggc gttaaaactt ttacgcgtga agtaaaacag cgcgtaaccc
     4681 agccagatgg tcagcagaat atgccgacgc cagtagcgat agcgggcatc aatttcatgt
     4741 ttatcagtca gtaatggcgc atcggcaggc gctttcagaa acggcaacat catcgaacct
     4801 tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca ggaaatgtgt
     4861 aatgtgccac ccagcgccgt tacgcgctcg cgcattccgg taaggccaaa accgtgttgc
     4921 ccggaatctg gcggtaaacc gctgccatcg tcttcaatca ccagcattaa ccgctcatca
     4981 tgctgccagc cttgcagcgt gaccgcgctg gcatcggcat gtttcacaat gttgttcaac
     5041 ccttcctggc agacgcgaaa tagcgtcacg cgctggtttt cgcttaacgc tgattcatcg
     5101 attcgccatt cgagatgact gacaatgccg cgcccttcca gctccatttc ccgcatcagt
     5161 gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa ccgccctaac
     5221 aagcggcgca ccgcgtcgta aacgcccagc gatagttgtt cgatgagctg cccgctctgt
     5281 ttcacgctgg cgttatccgc cgcgagccgc tggacaatgc ccgcttgagt tcgaatagca
     5341 gtaatggtct gaccgatatc atcgtgcagc tcacgtgcca catcacggcg cacgctctct
     5401 tcggtttcca gcaaccgttc agcgagatgc tgattgcgcg ccagttcctt ttgcagcgac
     5461 tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt cagactttgc
     5521 accagcagcg agagcaataa atccaccgga tgatcgcgcc aggtttgact ggcgatcagc
     5581 gcgatggcgt tcatcaacgt cgcaattagc gccccttgcc agccatagtg ccaggccagc
     5641 gcgataatcg gcagcgccag gcagaatggc gtaaagcgcg acagttcgtc cggcaatccc
     5701 aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg ccgcccacgc
     5761 cagttgatcg gttgagaaac taacgacggg ccgagcggca gccaggtgtt attggcgaga
     5821 tagtgccaga acaccagaca tatcggggcc agcgtcaggc cgccagttaa agtcagcaac
     5881 agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa caacgccgcc
     5941 gccgttaatg ccgccccttg tagcagcaag gtgcgccagt cacgttgatg tcgatagcgc
     6001 gagatcagcg ccacgggcag taacgtcagt aaactaccga tcatcaataa ggagaaatgg
     6061 gttaaaccga ccgcctgcgt taaccagtaa atcagcagcc actccgcgcc cagcaacacc
     6121 ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa cggaaataac
     6181 agcaccgcca tatcagggcg ctcaaccagg tgcaggctga tactccacag gcaaaaccat
     6241 gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa caacgtcttc
     6301 atcaccaacc atcaaacatg cggcgcgcca gttcaacgtc gttactgacg cccagttttt
     6361 ccatcagatt ggcgcgatgg acgtgtaccg ttttcggcga caagcccagt tcggcggcaa
     6421 tctctttcac cgccattcct tgcgccagtt tttctgccac ctggcgttcg cgtttggtta
     6481 gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga taacagccgc
     6541 ccgtggcaac cgtatgcacc gcagcaatca gttcgtcagg gctacaacgc ttggagagaa
     6601 agccgcgcgc ccccgcgtta agcgcctgct caaccagcgc cggactgtca tgaacagaga
     6661 gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg gagatatcgg
     6721 gcatggagat atcgcaaata cacacctgca caccgcgccc cggcaacccc gccagcgcct
     6781 cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc agcagctgcg
     6841 caaagccgga gcggacgata aggtgatcgt ctataagggc aacggtgatc atgatcttgt
     6901 cctggcgggt aataaaacgc gcttacctta acgataagcg cgatgttgtt caagccttga
     6961 gcggttactg aaaaaacacc gcaatcttgt taaacattgt cggatcggac tgattacgct
     7021 gcactttcac cacatcttcc agcttatcga tttggcttat catctgctcc agacgctggt
     7081 cgtcattgac cagtagccag atatggcttt tgtcgctgtc ctgaatcggc aggcagagaa
     7141 tgccttcaac gttaaaagcg cgacgggcaa aaaggccaca aacgtgggtc attacgcccg
     7201 gatggttgcg aacggtgagt tcgagaatca cgttgtcatg agttgtgttt tgcatggctt
     7261 attcccccac catttcagta ttcgccgcac ctggcggcac catcgggtac actttttctt
     7321 cggcatcaat gcgcacgtgg atcagcgccg gaccagggcg attgatgatt tcctgcaatg
     7381 cagcctgcgg atcggtttcg ttattcaaat cacaggtttc gaggccgaat ccggcggcaa
     7441 tctgcataaa gttgattttt cccggatagg tagcggcaaa aacgccctgc ttgtagaaca
     7501 gactctgttg ctgatgtacc agccccagcg cttcgttgtt catcagaatg attttgacat
     7561 ccagctgatt ttcgctagcg gtcgccatct cctgaatatt catcatcagg ctgccgtcgc
     7621 cggagaaaca caacactttg cgatccgggt tcgcaagcgc tgcgccaatc gccgcaggca
     7681 ggccaaagcc catcgtgccc agcccaccgg aggtcagcca ctggcgtggg cgattgagcg
     7741 gataagcctg cgccgtccac atctgatgct gacccacgtc ggtggtgata atcgcgctgt
     7801 catcgacaca ggcggcaaca gcgttgatca ggccgtaatg cgttaacgga tcgcacgctt
     7861 tcgggattgg acacgggaat tcacgctgca aatccgccac caactggtgc cactctgcac
     7921 gcggttgcgc ttccaccagc gggatcaact gcgccaacac gtcatcaaca tccgcctgaa
     7981 ttgccacatg cggttgcttg attttgccca gctctgcgcg gtcgatatcg acatgaataa
     8041 ttttggcatt cgggcagaac tgctcggttt tgccaatcgc ccggtcatca aaacgcgcgc
     8101 cgagaacaat cagcagatcc gcctcctgca aaatatagtt ggtgctgcgc acgccgtgca
     8161 tccctagcat acccagcgac agcggatgcg cttttggcaa catacccagc gccattaatg
     8221 tcattgtggt aggcagctgc gctttctccg ccagttcacg cactctcgca ggcgcattga
     8281 tcacaccacc gcccagataa agcaccgggc gtttggcagc attaatcatt gccgctgcgt
     8341 cacgaatgct ttcttcgcta aaggcgggcg cgatggcttt ttctgccacc gcgggctgtg
     8401 cttcaatctc aaaaacggcc gtttgcacat ccttaggaat gtctatccac accgggccag
     8461 ggcggcctga ttgcgcaata cggaaggcat cgctcatgac ctgcgggagt tcttcgatat
     8521 gtctgaccag atagttgtgt ttggtgatgg ggatagagat gccgtaggtg tcgacttcct
     8581 ggaaggcgtc ggtgccgatc atcgaggcgg gaacctgacc agtgatgcaa atcagcggga
     8641 tggagtccag ccgcgcatcg gcaatggcgg tcaccaggtt agtcgccccc ggtccgctac
     8701 aggccataca gaccgccggt ttaccgtcgg tgcgcgccat tccctgagcg ataaaccccg
     8761 cgccctgttc atggcgagcc agaatatggc ggatttgcgt gctttggctt aaggcatcgt
     8821 aaacaggcag gatagaaccg cccggaatgc ccgtcacaat cttaatgccc tgctgttcca
     8881 ggaaatgaac gataaattct gcgccggtaa agcgcttacg cgtcgatgtt gtgcccgaac
     8941 ttgccatgct ccagtccttt tcttctgggc cgactttccg ggaggtgctt aaacgaaaaa
     9001 ccccgcccgg tttgcgccgg cggggttttg gaatcgtgtg ttgttccagt ccctacggcg
     9061 cattgccgac gaccaccacc acacgcacga cgaccactgc ggcggatggc gcagttggta
     9121 gtagttttgc gttgagcatg gaagtagtca ttggggacct tgttggtttt gtgtttaaca
     9181 atatttatac aagcacagct ttacagggga gacaatggaa aatttttcag caagggaaaa
     9241 ttgaggggtt gatcacgttt tgtactgaat tgcag
//
LOCUS       DAZKJR010000181         9168 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKJR010000181 DAZKJR010000000
VERSION     DAZKJR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.182"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..614
                     /locus_tag="ACHOWS_004576"
     CDS             87..614
                     /locus_tag="ACHOWS_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0050049.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     KTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPLQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            672..905
                     /locus_tag="ACHOWS_004577"
     CDS             672..905
                     /locus_tag="ACHOWS_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFQ0050050.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDNGEHFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            969..2927
                     /locus_tag="ACHOWS_004578"
     CDS             969..2927
                     /locus_tag="ACHOWS_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0050051.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEDVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQMDATEHTTNLADAA"
     gene            2982..3416
                     /gene="psiB"
                     /locus_tag="ACHOWS_004579"
     CDS             2982..3416
                     /gene="psiB"
                     /locus_tag="ACHOWS_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFQ0050052.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3413..4132
                     /locus_tag="ACHOWS_004580"
     CDS             3413..4132
                     /locus_tag="ACHOWS_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFQ0050053.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGELCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENTI"
     gene            4129..4725
                     /locus_tag="ACHOWS_004581"
     CDS             4129..4725
                     /locus_tag="ACHOWS_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050054.1"
                     /translation="MMKSDEKYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVRAAQDWLCDRKLVDITDILR"
     gene            5186..5686
                     /locus_tag="ACHOWS_004582"
     CDS             5186..5686
                     /locus_tag="ACHOWS_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0050055.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            6416..6850
                     /locus_tag="ACHOWS_004583"
     CDS             6416..6850
                     /locus_tag="ACHOWS_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFQ0050056.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            6943..7209
                     /locus_tag="ACHOWS_004584"
     CDS             6943..7209
                     /locus_tag="ACHOWS_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050057.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            7399..7788
                     /locus_tag="ACHOWS_004585"
     CDS             7399..7788
                     /locus_tag="ACHOWS_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFQ0050058.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            7785..8747
                     /locus_tag="ACHOWS_004586"
     CDS             7785..8747
                     /locus_tag="ACHOWS_004586"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFQ0050059.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQYKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQ
                     QGIQKGRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            8809..9003
                     /locus_tag="ACHOWS_004587"
     CDS             8809..9003
                     /locus_tag="ACHOWS_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050060.1"
                     /translation="MTIAERLIQKGFDEGFKQGFKQGALEVAREIACRLRDIGWPPER
                     IQEATGLSGEELKKLFPDEQ"
     gene            complement(9034..>9168)
                     /locus_tag="ACHOWS_004588"
     CDS             complement(9034..>9168)
                     /locus_tag="ACHOWS_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0050061.1"
                     /translation="VDVTVWRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV
                     "
BASE COUNT         2018 a         2449 c         2749 g         1952 t
ORIGIN      
        1 aggagctttc gctcctctga aggggcacat gaacgccccg tctccggtat ccggtttatc
       61 tcacatcttt cagtcaggag gttctcatga gtgcacgtgg tatcaacaag gtcatcctcg
      121 tcgggcgtct gggcaatgat ccggaggtcc gttacatccc caacgggggc gcagtggcaa
      181 acctgcaggt ggccacgtca gaaagctggc gcgacaaaaa gacgggggag atgcgggagc
      241 agacggaatg gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggct ggtgaatatc
      301 tgcgcaaggg cgcgcaggtc tatatcgagg ggcaactccg aacccgtagc tgggaagata
      361 acggtatcac ccgttacgtc actgaaattc ttgttaagac cacgggcacc atgcagatgc
      421 tggggagtgc accactgcag aacgctcagg cacaaccgca gcctcagcag aatggtcagc
      481 cacagagtgc tgacgcgacg aaaaaaggtg gcgcgaaaac gaaaggccgt ggacgtaagg
      541 ccgcgcagcc cgagcctcag ccgcaaccgc cggaggggga ggattacggg ttttcagacg
      601 atatcccgtt ctgactggcc tgactgtgat aaccgccccg ccctgcgcgg ggcatcaccg
      661 gagatatgag gatgagcgaa tatttcagaa tacttcaggg actgcctgac ggtcctttta
      721 cccgcaaaca ggccgaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgaca
      781 acggagaaca ttttcgcctg gttgtccgta ataacggtgc aatggtctgg cgtacctgga
      841 attttgagga cggtgccggg tactggatga accatgtcat cagggatttc ggcattctta
      901 agtaagagag gtgccggacg ggtaggcgtc cggcagacat acaaccaccc cggtcaaagg
      961 agaaatatat gtcagttaca gagtctaagg caaaaacaga gcgcaaatcc agccgtaaac
     1021 ctgcaaaaac gcaggaaact gtcctgtctg ccctgctggc gcagacggag gatgtgagcg
     1081 ttccgctggc ttcactgatt aaatcaccgc tgaatgtgcg cacggtgccg tattctgcgg
     1141 agtccgtcag cgaactggct gagtccatta agggcgtcgg cctgctgcag aatctggttg
     1201 ttcatgccct gccaggtgac cgccacggtg tcgccgcagg tggtcgccga ctggcagcac
     1261 tcaacatgct ggcagagcgt ggcatcattc cggctgactg gcctgtccgc gtgaaaatta
     1321 ttccgcagga actggcgact gccgcatcaa tgactgaaaa cggtcatcgt cgggatatgc
     1381 accctgccga acagattgcc ggattccgcg caatggcgca ggaaggcaaa acacctgcac
     1441 aaatcggtga tttgctgggc tattcacccc gccacgttca gcgaatgctg aaactggcag
     1501 accttgcacc tgtcatcctt gatgcgctgg cagaagaccg catcaccaca gagcactgtc
     1561 aggcgctggc gctggagaac gacaccgcgc gtcaggtgca ggtgtttgaa gccgcctgcc
     1621 agtcaggatg gggcggtaaa ccggaagtgc agaccattcg tcgtctggtg accgaaagtg
     1681 aagtggcggt ggcggggaac agtaaattcc gcttcgtggg ggctgatgcc ttctcgccag
     1741 atgaactgcg cactgacctg ttcagcgacg acgagggtgg ctatgtggac tgcgtggcgc
     1801 tcgatgccgc cctgctggaa aaactccagg ctgtcgctga acaccttcgg gaagccgaag
     1861 gctgggaatg gtgcgccgga cgtatggagc ctgtcggttc ctgccgtgag gatgccggaa
     1921 cataccgcag cctgccggag ccggaagcgg tgctgacgga agctgaagaa gaacgcctga
     1981 acgaactgat gacgcgctac gacgcgctgg aaaaccagtg tgaggaatct gacctgctgg
     2041 aagcagaaat gaagctgatg cgctgcatgg cgaaggtcag ggcgtggacg ccggagatgc
     2101 gtgccggaag cggtgtggtg gtgtcctggc gttatggcaa cgtgtgtgtc cagcgtggtg
     2161 tgcagttgcg cagcgaggat gacgcggctg ccgacgctga ccgcacggaa caggtgcagg
     2221 agaaagcgtc agtggaggaa atcagtctgc cactgctgac gaaaatgtct tccgaacgca
     2281 cgctggcagt ccaggcggca ctgatgcagc aaccggacaa atctctggca ctgctggcat
     2341 ggacgctctg cctgaatgtg tttggcagcg gagcgtacag taaaccagca caaatcagcc
     2401 tggaatgtaa acattattcg ctgaccagcg atgcgccgtc agggaaggaa ggtgccgcgt
     2461 tcatggcgat gatggcagaa aaagcccgtc ttgcggccct gttaccggag ggatggtcac
     2521 gggacatgac gacgttcctt tcactcagtc aggaggtgct gttatccctg ctcagtttct
     2581 gcaccgcgtg cagcatccac ggcgttcaga cccgcgagtg tggtcacacg tcacgcagcc
     2641 cgctggacac gctggagagc gccatcggtt ttcacatgcg cgactggtgg cagccgacaa
     2701 aagcaaactt cttcggacac ctgaaaaagc cgcagattat cgcagccctg aatgatgccg
     2761 gactgtccgg tgctgcgcgg gacgcggaga aaatgaagaa aggcgatgcg gctgaacatg
     2821 cagagttcca tatgaaagac aaccgctggg tgcctggctg gatgtgtaca ccacgtccac
     2881 agatggatgc cactgaacac accactaacc tggctgatgc cgcctgatga acaaccacac
     2941 cgccccgccg gagacggggc ggcagcaagg gagataccgt gatgaaaaca gaactgaccc
     3001 tgaatgccct gcagtccatg aacgcacagg aatatgaaga tatccgggct gccggaagcg
     3061 atatgcgccg taatctcact cacgaggtga tgcgtgaagt ggacgcaccg gctaactgga
     3121 tgatgaatgg cgagtatggc agtgagttcg ggggcttttt ccccgtccag gttcgtttca
     3181 cgccagccca cgaacgtttc cacctggcat tatgttcgcc gggagacgtc tctcagctct
     3241 ggatgctggt tctggtgaat tgtggtggac agcctttcgc cgtcgttcag gtgcaacata
     3301 tcttcacgcc tgtcgctatc agtcacacgc tggcgcttgc cgcgacactt gatgcgcagg
     3361 ggtacagtgt taacgacatc atccatatcc tgatggcaga aggaggtcag gcatgagcgc
     3421 acgctcacgg gcactgatcc ccctcagcgc agaacaacag gccgccatgc aggctgtggc
     3481 tgtcaccgaa caacgtcgtc gtcagggacg cacactttca gcatggcctt atgccagcgc
     3541 tttctttcgc tgcctgaatg gcagtcgccg gatttcgctg accgatctcc gcttttttgc
     3601 tcctgcgctg acgaaggagg aatttcatgg caaccgtctc ctgtggctgg ctgccgtgga
     3661 taaactgatt gaaagttttg gtgaactctg tgttcttccc ctgccatccg atgcggggca
     3721 ccgtctgttc ccgtccgttc cttttcgtga aggtgagcgg cgtcgtcaga aaaccacgct
     3781 gacagagcag aaatacagcc gccagcggga acgtgaggca gaacgacggg aactggaata
     3841 ccagacatgt tttgctcagg cgcagattga ccttgcgttt catactcccg ccacggtcgg
     3901 aagctggttg tcccgctggt ctggtgttgt tgaggagcat gatctggaaa cgattttctg
     3961 ggggtggtgc gggcgttttc cgtcactgtc atcatttgac cggtttttct ggcaggagga
     4021 accactctgg cggctgattt ttgaagccgg tgaggccggt cgtggtgcac cggtacaggt
     4081 acgtgcactt gagcagtgga tgatcccgaa caaactggag aacacaatat gatgaaatca
     4141 gacgaaaaat atcaggttcc cgtgtggatg cgacctctgt tgccgttgct ctgcaatacc
     4201 ggtggtaatg atccggagga actgctgaat gatacagaaa ccaccgccag tgcgaatatt
     4261 gtccgttatg tactgatagt tgcagtgcgg tcgcagattg atctgctgca gctactgtac
     4321 aggaaaggac tgttgcgcac agagatacca ggtggctttt caccggaaga agcgcaggaa
     4381 ctcctggata atctggtgcg cagccatatc agcaaggcgc tgtcgggtga gcgaatggca
     4441 gcccgtgaca gaaatgccga tctggcctgg attcgccagc aactggtcga tgctgcctgg
     4501 tttgtccgtg ccacactgga agcgcatggg atgggcgtcg gaaatgagag tccctctgct
     4561 ccgccggaga caatgccgga catacagaca cgggaactgg tgatgctgat caaacgactg
     4621 gcatcttctc tgaaagcggt gaaaccggac agtagcgtag tgcgtgcggc gcaggactgg
     4681 ctgtgcgaca gaaaacttgt ggatatcaca gatattctcc ggtgaaccag gcatgtatat
     4741 ttttcgcagg gacacgccga tcatgatcgt ttcatatcag gggtggtctg gaggtcatgc
     4801 ggcgtgtcct ctgcactcgc cggaataagg aagtcgccgg cggctccgct tttacccggc
     4861 catgcggggc atggccttgt gggttttcag ttatgtggcc tcagcgtcgt gtgcgggctg
     4921 tgccgtgcct ccatcttagc cggctggcgg ggatgcaagg gtaaaatgca cgcggcggtc
     4981 acccggtccc tccttcccgt ctgccgtgat tttccggtcg ttcacccgct cagatttcgc
     5041 ggtctcaccc tccaactccc gccagccgtg tgcgtcaggc tgcggcttcc cttgcgccct
     5101 gtgcatcccc gccttatcgc ccggctttta tggaggcacg gcaccgcccg gtgccgcaga
     5161 acatgtgact atggaggatt cgggaatgtc tgttgttgca cctgctgtat acgttggaac
     5221 ctggcacaaa tacaactgtg gaagcatcgc cggacgctgg tttgacctga ccacgtttga
     5281 tgatgagcgc gactttttcg ccgcctgccg tgctcttcac caggatgaag ccgatcctga
     5341 actgatgttt caggattatg agggattccc ggggaatatg gcctctgaat gtcatatcaa
     5401 ctgggcctgg gttgaaggct tccgccaggc acgggatgaa ggctgcgaag aggcttatcg
     5461 tctctgggtg gatgataccg gtgagacgga ttttgacagc ttccgcgatg cctggtgggg
     5521 cgaggctgac agtgaggagg cttttgcggt tgagttcgtc agtgataccg gcctgctggc
     5581 tgacgtgccg gagacggtgg cgctctattt tgactatgag gcgtatgcgc gggatttatt
     5641 cctggactcc ttcaccttta ttgacggtca tgtgttccgt cggtgattta ctccccgctc
     5701 cggcgggggt atggcctgcc aggcccccgg aaaaccctgg caggcttacg cctgcctgaa
     5761 atgcaggagg gccgcgcctg cggcgctgcc cttcctgccc gtcatggagt gaggcatttt
     5821 tctgttatgt cgtttctgtt gttctgattt ccggaggcgg ggacaccttc ggctcccggc
     5881 tgcctggcgg catccggtcg tcaggcccga ctccgacagg cagctgctcg cgcacccgcc
     5941 agtctccgcc gaacccgctg gcgcggtttc gggctgccgc tacggtccga tgtctgaaaa
     6001 acctggcttg cagcgaccgt tccggtcgcg ccgcctctgt cgcgccgtga tctgcgctcc
     6061 ctgcacacct gacggtgtgt aaggaaactg cgcctgatgg cgcagttgtc tgcccttccg
     6121 ccgccacccc tgcggcctgt ttcccgctgt ggactttccg ggggctgaag cggccacaac
     6181 acgtgtcagc cgtccagggg gagatgtacc agccacaaaa ctttttgccg caagcgtcaa
     6241 aaacttttgc gtctgccccc ggacaactgc cccgtgccgt ggctgtcgct gccccatcgg
     6301 aaaggtccac tgcggggaga aaccccgaag aagtggacgg cagaaggggc tttcgcccct
     6361 ccgggggagc aaccggtcgg cccggcgacc tgattcagca gaaggagacg ttttcatgac
     6421 agtcagcagc acgatttccg ttttttgccg cgacggggtg ttccgtaccg tttactgcca
     6481 cctgcacggt gagccgacct ggaacggtcg catcctgcat acccactatg ccaccggtca
     6541 gcaggccgaa gccctggttg aacacggtga tatccgttgc ctcggtcccc gttgcgacaa
     6601 acccgccggg catacgcttc agaacccggt ggacggtgtg acggcttatt acggacgtga
     6661 cagtggtttc cggatggaca gtgaggcgcg tgagtaccgt tctttcatgg aggctattgc
     6721 cacggaaagc acagaagagg tgcgcttcca ttatgtgttc atcgacggct actggaaggt
     6781 gatgtaccgc acgccggaag gctggaagat gaaagcgctc gcgctggcac tgcgtcgctg
     6841 tccggaatga aaaaaagcag ggcgataaac cctgcctttc tccgccggcg cttccccgta
     6901 cggaagtccg gcatctcatt cactaactat ggagattatg ccatgagacc atcaattatc
     6961 ttcgcaaccg ccgagtatgt aaagcgtctg cgtgaagagt gcctgcggga gaacaaaccc
     7021 ctgcaccgcc atacccgctt cagacgccag gaactggcac cggatgagat taacccggac
     7081 gtcctggcga tgggcggtca tatcgccaga cgctgcagtg agcggaagcg ggtgcgtatc
     7141 cccgccatga aagtcagcga gtgggggcac ctgctccgtg cgcttgaaat tgaacgggtc
     7201 tgccactgat taccccctgc cgtctgtctg tgcaggtggc aggatatgtt caggtacttt
     7261 accaggagga accgtgagca agatgaaaaa catcatgacg tctatgtcgc atggtaccgg
     7321 attcctcctg ttccataaaa aacagagaag ggaaacaggt tctctttcat tcagggcccg
     7381 gacgggcagg agataaaaat gaagtcttgt tttgacactg acaaagattt tcagatgtca
     7441 tctgatgaag tcactttcaa tgctgacagc gttcgctcaa aactggaact attatccgaa
     7501 gaactaatgc accgttgtgg tcttgtttat gagctatatc agcgacgttt gagtgctgct
     7561 attgataatt ttgtggctac attgccgaca gaacaacatc tgctggtcct ggaactggcc
     7621 cgtgaggagt ttgattacct ttccgctgaa gagattgcag aagaaatacg ccgtgacgca
     7681 gaacgtggct attgttgtca cggctttgat cgcaattgct gtcctcttgg ttgtggggat
     7741 ttggacgatg attgctgcga tgctgctgat ttgatggagg aaccgtgagt aagaagaaaa
     7801 acaccacaac gcccacgccg catgatgccg cgttccggtc gttcctggcg aaccccgacg
     7861 tcgccagaga ttttctggaa ctgcatcttc cggcggagta ccggcagttg tgcgacctgt
     7921 ccacgctgaa gctggaaccc gcctcctttg ttgagccgga cctgcatcag tacgccagcg
     7981 atatcctctg gagtgtgaaa accaccgggg gtaaagacgg ctatgtttat acgctcatcg
     8041 agcaccagag caccgaaaat ctgtacatgc ctttccgcat gttacgttac agtgtggcgg
     8101 cgatgcagag acatctggag cagtacaaaa cattgccact ggtgattccg gtgctgttct
     8161 atcatggtga gcgcagcccg tacccgtaca gcatgaactg gctggactgt tttgaggatc
     8221 cggcgcttgc ggctaaaata tacacaaagc cgtttccgct ggttgatatc actgtcgttg
     8281 atgacaatga aatcatgaac catcgccgga tggccgcact gacgctgctg atgaagcata
     8341 tccgccatcg tgacatgatg gagctgctgg acaaactccc gcaggtcatg gtggaaattt
     8401 cagatgaaca ggtgcgtgtt ctgattcatt acatcgttaa cgcaggggac tctgtatcac
     8461 cggaatttat gcgggcgctg gctgagcgtc tgccgcagca tgaggataaa ctgatgacta
     8521 ttgccgaacg tcttgagcag caaggaatac agaaaggaat acagcaggga atacagcagg
     8581 gaatacagca gggaatacag cagggaatac agcagggaat acagaaaggc cgcatggagg
     8641 agaagcagga tattgcgcgt cagcttcaga aaacgggaat gccatctgca caaatctgtc
     8701 agatcaccgg actttccgaa gctgaactga agaacatcac tcactgatga gaaccacaaa
     8761 gcccggcaaa atgccgggtt ttttgtcagc aggagcagag caaacagcat gacgattgca
     8821 gaacgcctta tacagaaagg tttcgatgag ggttttaaac aaggttttaa acaaggggct
     8881 cttgaagtag cgcgggaaat agcctgccgg cttcgggata taggctggcc gccggaacgg
     8941 attcaggagg caaccggact ttccggtgaa gaactgaaaa agctgtttcc tgatgagcag
     9001 tagcctggca ttcagccagg ctgtgaatca cgctcacaca tcctctgccg tttcccccag
     9061 ttcattttcc agtcgcctga tgatatctgc cttacgcatc ccctgcatgt gaggcagcag
     9121 gtcacggact gaaagtgcct gggtggtcct ccagacggtg acatccac
//
LOCUS       DAZKJR010000182         9133 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKJR010000182 DAZKJR010000000
VERSION     DAZKJR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.183"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            189..551
                     /locus_tag="ACHOWS_004589"
     CDS             189..551
                     /locus_tag="ACHOWS_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050062.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(654..1112)
                     /gene="ybbJ"
                     /locus_tag="ACHOWS_004590"
     CDS             complement(654..1112)
                     /gene="ybbJ"
                     /locus_tag="ACHOWS_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0050063.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1109..2026)
                     /gene="qmcA"
                     /locus_tag="ACHOWS_004591"
     CDS             complement(1109..2026)
                     /gene="qmcA"
                     /locus_tag="ACHOWS_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0050064.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2172..2849
                     /gene="fetA"
                     /locus_tag="ACHOWS_004592"
     CDS             2172..2849
                     /gene="fetA"
                     /locus_tag="ACHOWS_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFQ0050065.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2836..3615
                     /gene="fetB"
                     /locus_tag="ACHOWS_004593"
     CDS             2836..3615
                     /gene="fetB"
                     /locus_tag="ACHOWS_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFQ0050066.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3678..4532)
                     /gene="cnoX"
                     /locus_tag="ACHOWS_004594"
     CDS             complement(3678..4532)
                     /gene="cnoX"
                     /locus_tag="ACHOWS_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFQ0050067.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4593..5402)
                     /gene="ybbO"
                     /locus_tag="ACHOWS_004595"
     CDS             complement(4593..5402)
                     /gene="ybbO"
                     /locus_tag="ACHOWS_004595"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFQ0050068.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHCGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5392..6015)
                     /gene="tesA"
                     /locus_tag="ACHOWS_004596"
     CDS             complement(5392..6015)
                     /gene="tesA"
                     /locus_tag="ACHOWS_004596"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFQ0050069.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5986..6672
                     /gene="ybbA"
                     /locus_tag="ACHOWS_004597"
     CDS             5986..6672
                     /gene="ybbA"
                     /locus_tag="ACHOWS_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFQ0050070.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6669..9083
                     /gene="ybbP"
                     /locus_tag="ACHOWS_004598"
     CDS             6669..9083
                     /gene="ybbP"
                     /locus_tag="ACHOWS_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFQ0050071.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2087 a         2339 c         2508 g         2199 t
ORIGIN      
        1 tcttatataa ataaaatcga aaatcatttt gtgctctttc aatgtaaaaa agagtaaaga
       61 cttgttttga taagcgaaat aaattctgtt tctggactaa acgtgaagcg gttcctgatg
      121 tgtgctaaaa tgcgtttctt tgataaatct caatttttaa gaggtttacg ccaggcagga
      181 aaatttaaat gaatgaattt gagaagattt ttaatgaaat gaatctggac cgggcattgt
      241 taccgatcct ttttcgttcg aaccggtcga cagtgtggaa gtatttaagc ggcgatagta
      301 cggctcctgc ctctgccatg tcgctgatca tgctgttgca gttgatccag aaacgaaacc
      361 ccgatctgtt agccgaatgg ttaacgcttt ctgatttcac cattccgcca gaagtttacc
      421 tggaccaacc tgattactgg aaagggtggg tgtataccca acacaaggtc aataaaaacg
      481 tcctggaata tctaaaaaaa cactatccgg atgaagacca aaaaagtatg ggtaaaggcc
      541 gggaagagta attttgcctc tactcggcta tatgccggat gcggcgcaag cgccttatct
      601 ggcctacgat tcgggcatat tttgtaggca tgataagacg cgccagcgtc gcatcaggcg
      661 atgaccgcac ggataatcag cgttatccct tctatcgcaa tcacctcaac atgcgtacct
      721 gcactgagat cctcgctggc gctgacaggc catgaactgt caccgacgcg catatgacca
      781 cgcccgttga ccagcggaga ttccagaaca aaacgtcggc caatcaactg ctgcccgcgc
      841 tggtttaaat gactgtcgct gtgcttttgt tcgcgcaccc gccgcgacaa ccatttccac
      901 cacagccagg cggcgagcag cgtcaggacg gcaaacatta ccccttgcca ctcccaaccc
      961 agcggcagca accagaccac cagaccggta atcaccgctg ccacgccgct ccacaacaga
     1021 taaccattgc cgcccaacat ctcggctgcc agcagcaaac cgccgagact tagccagaaa
     1081 atatgtggat gaacgaccat taactccatc atggctgagt ccgtttgttg gcgctgtctt
     1141 tcaccagctc agcaatcccg gcaatcgacc ccatcaggct gctggcctct aatggcatca
     1201 tcaccacttt gctgttactg gacgaaccga tctgctgtaa cgcttcggtg tatttctgtg
     1261 cgacgaagta gttcaccgcc tgaatatcac cggaggcaat ggcttcagac accattttag
     1321 tggcgcgggc ttctgcttca gcggaacgtt cacgcgcttc agcctgtaaa aacgccgact
     1381 gacgttcgcc ttccgctttc aggatttgcg actgtttttc accttcggct ttgaggattt
     1441 ccgcctgacg gatcccttcc gcttcaagga tgtaagcgcg tttggtacgt tccgctttca
     1501 tctgcgcgtt cattgaagag ataagctctg ccggtgggcg cacgtcgcga atttcaatac
     1561 gggtgacttt aatcccccac gggttggtgg cctcatcgac aatacgcagc aggcgtgagt
     1621 tgatgctgtc gcgctgagag agcatttcgt caagttccat tgaacccagc acggtacgga
     1681 tgttagtcat ggtcaggttg atgatcgcca gctccagatt gctgacttca taagccgcgc
     1741 gcggcgcgtc aatcacctga ataaagcaca cggcgtcgat ggtaacgttg gcgttatctt
     1801 tcgagataac ttcctgggaa gggatatcga gcacttgctc catcatattg atcttgcgac
     1861 caatgcgatc cataaacggc accaccagac tgagccccgg ctgtaacgtt ttggtatagc
     1921 gaccaaaacg ttctactgtc cactgatagc cctgcggcac gattttgacg cccgcgccga
     1981 caatgaccag cgcgacaaaa atgagaatcg ggataaagat aagcatcgga aaaacctcct
     2041 gttgtaccgt ccataatcag caaaattgct gcttgattaa acaaattata cctgattact
     2101 gaaagagagt tcccccttat tcctgcgaag gataaactgt ttttagtaaa aatcagaaaa
     2161 agggaacagt gatgcaggaa aatagtcctt tgcttcagct acaaaacgta ggatatctgg
     2221 cgggtgatgc gaagattctt aataacatca atttttcgct gcgtgctggc gaatttaagt
     2281 taattaccgg tccttctggt tgtggcaaaa gtacgctgct aaaaatagtt gcttcattga
     2341 tcagcccaac cagcggaacg ttactgtttg aaggtgagga tgtcagcaca ctaaagccag
     2401 aaatctaccg ccagcaagtc tcttattgcg cccagacgcc gacgctgttt ggcgacacgg
     2461 tatacgataa tctgatcttt ccctggcaga tccgtaacca gcagcctgac ccagccattt
     2521 ttctcgattt tctcgaacgc ttcgccttac cggacaccat tttgacgaag aatatcgccg
     2581 agctatctgg tggtgaaaaa caacgcatct cattgattcg taacctgcaa tttatgccga
     2641 aggttttatt gctggatgaa ataaccagtg cgctggacga aagtaataaa cataacgtta
     2701 atgagatgat ccaccgttat gtgcgcgagc aaaatattgc cgtgctgtgg gtgacacacg
     2761 ataaagacga aattaatcat gcggataaag tgattacact gcaaccgcat gccggagaaa
     2821 tgcaggaagc acgctatgaa ctcgcataat attactaacg aatcattagc actggcattg
     2881 atgctggtgg tggtggcaat cttaattagc cataaagaaa aactggcgct ggagaaagat
     2941 attctctgga gcgtcgggcg cgcgataatc cagctgatta ttgtcggcta tgtgctgaag
     3001 tatattttca gcgtggatga tgccagcctg acattattga tggtgttatt tatctgcttt
     3061 aatgcggcgt ggaacgcgca aaaacgcagt aaatatattg ctaaagcttt tatctcatcg
     3121 tttattgcca ttacggtcgg ggcgggaatt accctggcgg tgctgattct ctcagggtcg
     3181 attgaattta tcccgatgca ggtgatccct atcgccggga tgatagccgg caacgccatg
     3241 gtagcggtgg ggttgtgtta caacaattta gggcaacggg tcattagcga acagcaacag
     3301 attcaggaga aactgagtct tggcgcgacg ccgaaacagg cttcagcgat attgattcgc
     3361 gacagtattc gcgcggcttt aattccgacg gtcgattcag caaaaacggt aggcttagtg
     3421 agtttaccag gaatgatgtc cgggctgata tttgccggga ttgatccggt gaaggcgatt
     3481 aaatatcaga ttatggtgac ctttatgctg ctctcaaccg ccagcttgtc gaccattatt
     3541 gcctgctatt taacctatcg taagttttac aattcgcgcc accaattggt ggtgacgcaa
     3601 ttgaagaaga aatgatgcga tgccggacgc gcagcatcgc atccggcgtt gtggttcatg
     3661 tgccgatcaa cggaaactca atacaacaac gcgtacagct ggcggcgata cttcgacgcc
     3721 agtgcatcac ccgtacccag cgcagcgagg atctcctgga atgttttacg tgtctgacca
     3781 tctgcggcgg tgagatcttt acgcagatgc ccaaacagta actccagcgc ctcttcattg
     3841 cgcccaacct ggtgcagttg cagtgccagt tgtgtcgcca gtgcggcatc ttctggattc
     3901 tccgccacct gctgttgcaa ctgttgaatt tccggcgtat cagccgcctg cttcagcagt
     3961 tcgatttgcg ccaccagccc ctgatagcgg gtgtcctgat cctgcaacgg aatggttttc
     4021 agcacggctt ctgcatcttc agaacggttc agcgcaatca gcgtttctgc cagcagcagg
     4081 ccgatctccc cgttctgatt cgacaactgc caggcgtctt tcagcaatgg caaggcgtcg
     4141 gtgtaattgc cttcctgcat cagctgcatc gcctgctgcg ctttcagctc ttcttcgcgc
     4201 ggcagcactt tatccagcag ggcgcggatc gcctcttccg gttgcggccc ctggaagcca
     4261 tctaccggtt gcccgttctg gaacagatac acggtcggaa tcgcgcgcag accaaactgg
     4321 gcggcaatca tctgctccgc gtcgcagtcc agcttcgcca gaataaattg cccgttgtac
     4381 tgcgccgcga ggctttccag gatcggggtt aactgcaaac agtgctggct acgttcagac
     4441 caaaaataga acagcaccgg agtggtcatc gactgttcaa gaacctgttg caggttagat
     4501 tcgttaatgt tgacaatatt ttctacggac atggagtcgc tctctgttgt cgatattttc
     4561 tttgacatgg gggcttaagc gcgcgcttca actcacccct gcaatatttt gtccatcacg
     4621 cgccccggca gcaggcgctt aagcaccatt accgcccagg tcaccagcgt taccggatag
     4681 cgcatcttcg gcttctcgct aataaaagca tggcgtactt tgtccaccac cgcttccggt
     4741 cccaacgtaa agcgggcggc gatgccggga ttttcgactg gtttatcact ttgcgtctgg
     4801 ttgacgttgt cggtgaagcg agtacgaatg ggaccgggtt cgatcaggct gactttaatt
     4861 ccacagtggc gtagctccat gcgcagcgcg tctgaccacg cctccagcgc atatttactg
     4921 gccgcgtaag caccacgacc cggcgtggag attaatccca tcaccgatga ggtcatcaca
     4981 atacgccctt caccgtgcgg taacatcgcg ggtaacaggc gcatggtgag ctggtgtgcg
     5041 ccgaaaaagt tggcggaaaa ctgctgttcc atctgcgcac ggctgatggt ggaaaggggg
     5101 ccatacatgc cgaatccggc attgttaaag atcccataca gacaattatc ggtcagggcg
     5161 atcacctcgt cggctgcgcg atcaacactt tctggtgaat ccagatcgat caacacgccg
     5221 gtaaatccca tgctgttcat gcgctcaaca tcatccggtt tccggcaacc tgccagcaca
     5281 tgaaaaccct ggcgttttaa ttcgagcgcg ctttccaggc caattccact ggaacatccg
     5341 gtaattaaga ccgatttttg cataacttta cctgtcagga tctccgttgc tttatgagtc
     5401 atgatttact aaaggctgca actgcttcgc catccagtcg gcaataaacg gctgggcgtc
     5461 gcggttggga tgaataccgt catcctgcat ccattgtggt ttgaggtaga cctcttccat
     5521 aaaaaagggc agcagcggaa catcaaactc tttggcgagt ttggggtaaa tggcgctaaa
     5581 ggcttcatta taacggcgac catagtttgc aggcagacgt atttgcatta acaatggttc
     5641 agcgttggcg gctttgacat cctgcaatat ctggcgcagc gtttgctcgg tttgttgtgg
     5701 ctgaaaacca cgcaaaccgt cattgccgcc cagttctacc agcacccagc gcggctgatg
     5761 ctgtttcagc agagccggaa ggcgcgccag cccttgttgc gaggtgtcgc cgctgatgct
     5821 ggcattgact accgacgttt tactctgcca cttatcgttc aacaaggcag gccaggccgc
     5881 gctggcagac attcgatacc cggcgctcag gctatcaccc agaatcaata acgtgtccgc
     5941 tgcggcggca cggaaggtta acaggaccag gaacaggaag ggcaaatgcc agcggaaaac
     6001 attgttgaag ttcatcatct taagaagtcc gtcggtcagg gggagcatga actctccatc
     6061 ctcaccggag ttgagctggt tgtcaaacgt ggcgagacca tcgcactggt gggcgagtcg
     6121 ggatcgggta agtcaacttt gctggcgatc ctcgccgggc tggatgacgg cagcagtggc
     6181 gaagtgagtc tggtgggaca accgctacat aatatggacg aagaagcgcg ggcaaagttg
     6241 cgcgcgaagc acgtcggctt tgtttttcag tcatttatgt taattcctac ccttaacgcg
     6301 ctggaaaacg tcgagcttcc ggcgctgctg cgcggtgaga gtagcgcgga aagtcgtaac
     6361 ggggcgaaag cgttgctcga acagttaggg ctgggtaaac gtctggatca tcttccggca
     6421 cagctttccg gcggtgaaca gcaacgagtg gcgctggcac gagcctttaa cggtcgacct
     6481 gatgtgctgt ttgccgacga acccaccggc aatcttgacc gacagacggg cgataaaatt
     6541 gccgacctgc tattttccct caaccgtgag catggcacca cgttgattat ggtgacccac
     6601 gacctgcaac tggcggcacg ctgcgaccgc tgcttacggc tggtgaacgg gcaattgcag
     6661 gaggaagcat gattgcacgt tggttctggc gcgaatggcg ctcgccgtcg ctattaattg
     6721 tctggctggc gctaagcctg gcggtggcct gcgtgctggc gctgggcaat atcagcgatc
     6781 gcatggagaa gggcttaagc cagcaaagcc gggagtttat ggcgggcgat cgggcgttgc
     6841 gcagttcacg cgaagtgcca caagcgtggc tggaggaagc gcaaaagcgc ggcctgaaag
     6901 tcggcaagca gctgactttc gccacaatga cctttgcagg cgacacaccg cagctggcga
     6961 acgtcaaagc ggtggatgat atctacccga tgtatggcga tctgcaaact aatccccctg
     7021 gcctgaaacc gcaggcgggc agcgtattgc tggccccacg cctgatggca ctgcttaacc
     7081 tgaaaacagg cgacaccatc gacgtgggtg atgccacctt gcggattgcc ggagaagtga
     7141 ttcaggaacc ggattccggt tttaacccct tccagatggc tccgcgtctg atgatgaatc
     7201 tggcggatgt cgataaaacc ggagccgtgc agccggggag tcgggtcacc tggcgctata
     7261 aattcggcgg cagcgagaac cagctcgacg gctatgagaa atggttgtta ccccagctta
     7321 aacctgaaca acgctggtac ggtctggaac aggacgaagg cgcgctgggg cgatcgatag
     7381 aacgttcgca acagttcctg ctgctttcgg cgcttctgac cttgctgctg gcggtggcgg
     7441 cggttgcggt ggcgatgaat cattactgtc gcagtcgcta cgacttggtg gcgatcctca
     7501 agacgctggg ggcagggcga gcgcaactgc gtaagttaat cgtcggtcag tggttgatgg
     7561 tgctggcgct ttcagccgtt accggtggtg cgataggcct gttgttcgaa aacgtgttga
     7621 tggtgctgct caagcccgtt ttgcctgctg cactaccgcc agccagcctc tggccgtggt
     7681 tgtgggcgct gggcaccatg acggtcatct cgctgctggt ggggctacga ccgtaccgct
     7741 tgttgctggc aacgcagcct ttacgcgtat tacgtaatga tgtggtcgcg aacgtctggc
     7801 cgctgaagtt ttatctgccg attgtcagtg tggtggttgt gctgctgctc gccggattaa
     7861 tgggtggcag catgctgctt tgggcggtgc tggcgggcgc ggtagtactg gctttgctgt
     7921 gcggtgtgct gggctggatg ctgctgaatg tacttcgccg catgacgctg aaatcgctac
     7981 ctctgcgcct ggcggttagc cgcctgttac gtcagccgtg gtcaacgtta agccagcttt
     8041 cggcattttc gctctccttt atgctgctgg cgctgttgct ggtattgcgt ggcgatctgc
     8101 tcgaccgctg gcagcagcag ctacctccag aaagcccgaa ctacttttta attaacatcg
     8161 ccacagaaca ggtagcaccg ctaaaagcgt tcctcgcgga acatcagata gtcccggaat
     8221 cgttttatcc ggtggtgcgg gcgcggctga cggcgattaa cgataagccg acagaaggca
     8281 atgaagatga ggcgctcaat cgcgaactca atcttacctg gcaaaatacg cggcccgatc
     8341 ataatccgat tgtcgccggt aactggccgc caaaagccga tgaagtgtcg atggaagagg
     8401 ggctggcgaa acgcttaaac gttgccctcg gtgataccgt gacttttatg ggcgataccc
     8461 aggagttccg cgctaaagtg accagcctgc gcaaagtgga ctgggaaagt ctgcggccta
     8521 atttctattt tattttccct gaaggggcat tagacgggca accgcagagc tggcttacca
     8581 gtttccgctg ggagaatggc aacggcatgt tgacgcaact caaccgccag ttccctacta
     8641 ttagcctgtt agacattggc gcgattttaa aacaggtcgg tcaggtgctg gagcaggtaa
     8701 gtcgggcgct ggaggtgatg gtggtgctgg tcaccgcctg cggtatgttg ctgttgctgg
     8761 cacaggtgca ggtgggaatg cgtcagcgtc atcaggagct ggtggtgtgg cgcacactcg
     8821 gtgcggggaa aaaactgctg cgtaccacgt tgtggtgtga gttcgccatg cttgggtttg
     8881 tttctggcct ggtagccgca attggtgcgg aaacggcgct ggcagtgttg caggcgaaag
     8941 tgtttgattt tccgtgggag ccagactggc gattgtggat tgtgctgccg tgcagcggtg
     9001 cactgctgct gtcgcttttc ggcggctggc tgggtgcgcg actggttaag ggtaaggcgc
     9061 tgttcaggca gtttgcggga tgatgagagt aaataagggg cgttctggtt gaatggaacg
     9121 ccttgtttaa att
//
LOCUS       DAZKJR010000183         8984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKJR010000183 DAZKJR010000000
VERSION     DAZKJR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.184"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(129..1289)
                     /locus_tag="ACHOWS_004599"
     CDS             complement(129..1289)
                     /locus_tag="ACHOWS_004599"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFQ0050072.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1295..1966)
                     /locus_tag="ACHOWS_004600"
     CDS             complement(1295..1966)
                     /locus_tag="ACHOWS_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFQ0050073.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCIAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2114..3595)
                     /gene="tolC"
                     /locus_tag="ACHOWS_004601"
     CDS             complement(2114..3595)
                     /gene="tolC"
                     /locus_tag="ACHOWS_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFQ0050074.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3800..4429
                     /gene="nudF"
                     /locus_tag="ACHOWS_004602"
     CDS             3800..4429
                     /gene="nudF"
                     /locus_tag="ACHOWS_004602"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFQ0050075.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4430..4852
                     /locus_tag="ACHOWS_004603"
     CDS             4430..4852
                     /locus_tag="ACHOWS_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFQ0050076.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4877..5704
                     /gene="cpdA"
                     /locus_tag="ACHOWS_004604"
     CDS             4877..5704
                     /gene="cpdA"
                     /locus_tag="ACHOWS_004604"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFQ0050077.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            5704..6285
                     /gene="yqiA"
                     /locus_tag="ACHOWS_004605"
     CDS             5704..6285
                     /gene="yqiA"
                     /locus_tag="ACHOWS_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFQ0050078.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6314..8206
                     /gene="parE"
                     /locus_tag="ACHOWS_004606"
     CDS             6314..8206
                     /gene="parE"
                     /locus_tag="ACHOWS_004606"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0050079.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFTLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8251..>8984)
                     /locus_tag="ACHOWS_004607"
     CDS             complement(8251..>8984)
                     /locus_tag="ACHOWS_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFQ0050080.1"
                     /translation="PSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQK
                     VPCLYENTISMLYELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKR
                     NGIDFSSSALRKKEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKY
                     DAIVFIDDGQANIHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKM
                     ATDWKTLAGSLATLFPDRNKLCPIK"
BASE COUNT         2108 a         2200 c         2403 g         2273 t
ORIGIN      
        1 gatttaacat taaattgagt attaatgtta aataaaggtt ggatatgtat gtgtttatat
       61 attgatggga attatgaatg gtggattata agagattgcc ccgcttatcg gggcaatatt
      121 aagctgtatt actcaacaaa aatatgcgga taaaaccgag acatatcctg ggtgatcaat
      181 gcacggtctt cacgaatgcc aattccggca ggttgatcgt tcaccagcca gctaccaatc
      241 agcatatagc tgtcgccgaa tttcggtaac gggtggaatt gctgaacaat catcccttct
      301 tcgccatacg gaccttccgc tgcttcaatg gttttgccgt tctcaatgat cgacacgttt
      361 gcgccttcac gggagaagat cggtttaacc acatattttt ccatttgcgg atgatcatct
      421 tccgcaaaat aagcaggcag caggttcggg tgattcggga acatctccca cagtagcggc
      481 agaagcgctt tgttggagat aatgctcttc cacgccggtt ccagccagcg tacgcctgca
      541 tcctccagct tggtggagaa catctcgcgc agcataaatt cccacggata cagcttgaac
      601 aggttggaaa ttacctgatc ctgtaaatcc gtgaactgac ctttttcgcc taaaccgata
      661 tcgtcgatgt agaggaattc ggtagcgatt tcagcttccg tcgcgcagtc ctgcaaatac
      721 tgaatggttc cgcgatcttc caccgtgtcg cgacagcagg tgagatgcag caactggaag
      781 ccatactgtt cacgcaactc gacaaagcga tcgattagct tttcttgcag actgttaaac
      841 tggtcgctgc cctccggcaa gttaccggcg ttaagctgat cttccagcca gatccactga
      901 aagaacgccg cctcgtatag tgacgttggt gtatcggcgt tattttccag cagtttaggt
      961 tcgccagtgc catcccacgc cagatcaaga cgcgaataaa gcgatggctg gtgagtcagc
     1021 catgactgac gcacaaaact ccaggtgtgt tttggaatgc ggaatttggt catcagctca
     1081 tcgctggcga tcactttttc caccactttc aggcacatct ggtgcagttc ggcggtgact
     1141 tcttccagct tttcaacctg ggcgagggtc aacttgtagt aagcatcttc acaccagtac
     1201 ggctcgccgt acatggtgtg aaaattgaaa ccgtattcgt gggctttctc acgccagtcc
     1261 gggcgctcgg taatactgac tctttccatc ggtatcagcc acccattgaa cgagaagagg
     1321 tgccggttgc gctacgctgc atagtgcttt gtttggcaac agattcacca aaaccgccac
     1381 gggtaacggt agtggtggtc gccggttttg gtgccatggc tgtcttcggt acggtcatgg
     1441 tgcggcctgg ctgggctgcg ccatagtttt tacccgtcgc gtcggtatat ttaccgtaag
     1501 ccgggctggc tgggtttttc gaggagaaca gcggctgctg tgcaaatccc gcaccgccgc
     1561 ccatcagacg acccatcatg taaccggcca tcagcggcat ccagaaactc ccgctggatt
     1621 gctgggcctg cgcctggttt tccggtgcca tgccagcctg ggctggtgcc tgctggcact
     1681 gaccttcgcc aaattcagca atacagtctt cacgagtggc gtatttcggc gcagtacgtt
     1741 cggcttcttt cagcgcattg ttgtacgcgg tggtacattc ggcgcttttg cctgggtttg
     1801 cagctgaaca gtcgtcagca ttttgataga gagacactgt ttcatcactc ttttcacagc
     1861 cagccagcat aaaaacagtg gcaaccgcga gagcgactgg tgtcagatgg cgtgcgctcc
     1921 agtttttgcg gaacgatgcg tggcgtatgg attttgtccg tttcattttt gtcttccggg
     1981 accagtggta aatacccatc agaatagagg atggctggtc gaaattgaag cgagaagggg
     2041 gaagaatgcg gcagataacc cgtatcttta cgttgcctta cgttcagacg gggccgaagc
     2101 cccgtcgtcg tcatcagtta cggaaagggt tatgaccgtt actggtggta gtgcgtgcgg
     2161 atgtttgctg aacgacgggt gccgggctat caggcgcata accatcagca atagcattct
     2221 gttccggcgt ttgcggggca acgttttccg gattagtgga aaccggtttg ctcagcgcat
     2281 tgttcagtgc cagaagatcc tgctcgttca acgtacccag agctgattta atattcaact
     2341 ggttaatcag gtagttataa cgcgcattcg ccagctcttg cttggcgttg tacagcgtgg
     2401 tggtcgcatc caacacatca acaatggtac gcgtaccgac cgagtaaccc gcttccatcg
     2461 cgtctaatga gctttgagcg gaaactacgg cttgtttgta ggcgttaatg ctactgatag
     2521 aagcattaat gttgttgaag gaggaacgta cggtttgcac gacgctacga tgcgcgcttt
     2581 ccagttgctc gctggcacca acaaagttgt actgggcctg tttcacctgc gagttaacca
     2641 ttccgccctg ataaatcggc agcgagaagc tcaggccaac tttgttctgg cccatattgc
     2701 tgtcgtcata ctgggtacca gcggcaccac gggttttcga accgctataa gaggtgtcag
     2761 aaatcccggt agaagccgtt aaatccagag tcggtaagtg accatcctgc gcctggcgaa
     2821 tttgctcgcg cgccaggtcc tggctcaagc gcgcctgtaa cagcgacagg ttgcgttttt
     2881 cggcttcttt cagcagcgtg ttaaccggct gtggtttgtc ggttttaaag ttttcgacat
     2941 tcagcgccgc cagttctgga tagtagttac cggtgatctg gcgcagctgc tctaccgcgt
     3001 tatcaaggtt attacgtgcg gtcacttcgt tcgccagcac ggtatcgtac tgtgcgcggg
     3061 cgttctgcac gtcagtgatc gctaccaggc ccacgttaaa acgttgggtg gtttgatcta
     3121 attgacggta gatcgcttct ttttgcgcct gtgtatagga aagaacgtca atagcattca
     3181 acacgttgaa ataagcggtc gcggtgttga ggatcaaggt ttgttgatcg gtctgatacg
     3241 tgacgtcctg aatcccagct gctttttcct gcagcgttaa cgcacgccat ttcgacatat
     3301 caaaaatgga ttgagttaac tgcagggacg cactggtcgc gttagagttg atgccgttcg
     3361 cgtcgcggta gccgttgcta taggtgtaat ctgcacctaa acctagctgt ggcagtaatg
     3421 gactgcgcgc ttcattaatt ttttcaaagg cagcatcacg atcggcggca gacttacgca
     3481 attccgggtt actaaggcgt gcttgctgat aaacttgcat caggttctcg gcctggctca
     3541 acgaactgaa accagaaagg ctcaggccga taagaatggg gagcaatttc ttcatttgca
     3601 ttccttgtgg tgaagcagta tttagcgcga tcaaactgta aaattattgc ggattctagc
     3661 agaagccgct accgcaaaag gttggcgtta cgtgccatag ggcgttaatg tgcctcaatt
     3721 taacacatga ctgctcaaac ggcagtcaaa cgtcgctgaa attcacattt aattcactat
     3781 tagtgccagg acattaacaa tgcttaagcc agacaacctg cccgttacat ttggcaaaaa
     3841 cgatgtagaa attattgcac gagaaacact ttatcgcggc tttttttcat tagatcttta
     3901 tagatttcgt catcgtctat tcaacgggca aatgagtcat gaggtacggc gggaaatttt
     3961 tgagcgcggt cacgccgcag tcttgctacc ctttgaccca gtgcgtgatg aagttgtgct
     4021 gattgagcag attcgaattg ccgcgtacga cactagcgaa accccctggc tactggagat
     4081 ggttgccggg atgattgaag agggtgaaag tgtggaagat gttgcccgtc gcgaagcgat
     4141 tgaagaggcg ggactgatag tcaaacggac caaaccggtg ttaagtttcc tggcaagccc
     4201 ggggggcacc agtgagcgtt cgtcaattat ggtgggcgaa gtggacgcca cgaccgcaag
     4261 cggtattcac ggtctggctg atgaaaacga agatattcgc gttcatgtgg taagccggga
     4321 acaggcatac cagtgggtag aagaggggaa aatcgacaac gcagcgtcgg tcatcgcttt
     4381 gcaatggctg cagctgcatc atcaagcgtt aaaaaatgag tgggcataaa tgaagcgtta
     4441 cacacctgac tttcctgaaa tgatgcgcct gtgcgagatg aacttttcac aattgcgccg
     4501 tttgttaccg cgcaatgacg cacccggcga aactgtaagc tatcaggtgg caaacgcaca
     4561 atatcggctg acgattgtgg aatcgacccg atacactacc ctggtgacaa tagaacagac
     4621 tgcgcccgcg atcagttact ggagccttcc gtcaatgacg gtgcgtctgt atcatgacgc
     4681 gatggtggct gaagtgtgtt caagccagca gatttttcgc ttcaaagcgc ggtatgatta
     4741 tcctaataaa aagttgcatc aacgcgacga aaagcatcaa attaaccagt ttttagcgga
     4801 ctggttgcga tactgtttag cacatggagc gatggcgatt ccggtttact agcgtcgtga
     4861 aacctaagga caccatttgg aaagcctgtt aacccttcct ctggctggtg aggccagagt
     4921 caggatttta caaattaccg acactcacct gtttgcacaa aagcacgaag ccctgttagg
     4981 ggtaaacacc tgggaaagct accaggcggt gctggaggcg attcgtccac accagcacga
     5041 attcgacctg attgtcgcga caggtgattt agcgcaggat caatcctctg cggcctatca
     5101 gcatttcgct gaaggcatcg caagttttcg tgcgccctgc gtctggctgc cgggcaacca
     5161 cgatttccag cccgcgatgt acagcgcgtt acaggatgcg ggtatctccc cggcgaagcg
     5221 ggtgtttatt ggtgagcaat ggcaaatcct gttgctggat agccaggtgt ttggcgtgcc
     5281 gcacggtgag ctgagcgagt ttcagcttga gtggctggaa cgtaaactgg ccgatgcgcc
     5341 agaacgccat acgttgctgc tgctgcatca tcatccgcta cctgcgggtt gtagttggct
     5401 tgatcaacac agcctgcgta acgcgggcga actggatacc gtgctggcga agtttccgca
     5461 cgtcaaatac ttgctgtgcg gtcatattca tcaggagctg gatctcgact ggaacggtcg
     5521 ccgcctgctg gcaacgccgt cgacctgtgt gcagtttaag ccgcactgtt ccaactttac
     5581 gctggacacc atcgcgcccg gctggcgtac tctcgagtta catgctgatg gcacgctgac
     5641 caccgaggtg catcgcctgg cggacacacg tttccaacct gataccgctt cagaaggcta
     5701 ctgatgtcta cgcttcttta tttacacggt ttcaacagct cgccgcgctc tgcaaaagcg
     5761 agcttgttaa aaaactggct tgcggaacat caccctgacg ttgagatgat catcccgcag
     5821 ttgccgccgt atccttccga tgcggcagaa ctgctggaat ccattgtcct ggaacatggc
     5881 ggcgattcgc tgggtattgt cggttcgtca ctggggggat attacgccac ctggttgtcg
     5941 caatgtttta tgctgcccgc agtggtggta aacccggcgg tacgcccgtt tgaactgctg
     6001 acggactatc tcggtcagaa cgagaacccc tacaccgggc agcaatatgt gctagagtca
     6061 cgccatattt acgatctcaa agtcatgcag attgacccgc tggaagcgcc ggatttgatc
     6121 tggctgctgc aacagacggg agatgaagtg ctggattacc gccaggcggt ggcgtactac
     6181 gcttcctgcc gccagactgt catagaaggc ggcaatcacg cattcacggg cttcgaagat
     6241 tatttcaacc cgatcgtcga ttttcttggt ctgcaccatc tctgacgatc aactcgaatt
     6301 gctaacttaa accatgacgc aaacttataa cgctgatgcc attgaggtac tcaccgggct
     6361 tgagccagtt cgccgccgtc cggggatgta taccgatacc actcgcccta accatttggg
     6421 gcaagaagtt attgataaca gtgtcgatga agcactggcg ggtcacgcaa aacgcgtgga
     6481 cgtaatctta catgctgacc agtcgttaga agtgattgac gatgggcgcg ggatgccggt
     6541 ggatattcac ccggaagagg gtgtaccggc ggttgaactg attctttgcc gtctgcatgc
     6601 aggcggtaaa ttctctaaca aaaattacca gttctctggc ggcctgcatg gcgtggggat
     6661 ttcggtggtt aacgccctgt cgaagcgcgt agaagttaac gtacgccgcg atggtcaggt
     6721 ttataacatc gcctttgaaa atggcgaaaa ggtgcaggat ttacaggttg tcggcacttg
     6781 cggtaagcgc aataccggta ccagcgtgca cttctggccg gatgaaacct tctttgacag
     6841 cccgcgtttt tctgtttcac gcctgacgca tgtgctgaag gccaaagcag tactgtgccc
     6901 tggtgttgag atcaccttta aagatgagat caacaacacc gaacagcgct ggtgctatca
     6961 ggacggtctg aatgattacc tggcggaagc ggtaaacggt ttaccgacgc tgccagaaaa
     7021 accgtttatc ggtaatttcg ctggcgatac tgaagcggtg gactgggcgc tactgtggct
     7081 accggaaggc ggtgaactgc tgaccgaaag ctacgtcaac ctgatcccaa cgatgcaggg
     7141 cggtacccat gttaatggcc tgcgtcaggg cctgctggac gcgatgcgtg aattctgtga
     7201 ataccgcaac attctgccgc gcggtgtaaa gctgtcggcg gaagatatct gggatcgctg
     7261 cgcctatgtg ctgtcagtaa aaatgcagga tccgcagttt gccgggcaga ccaaagaacg
     7321 tctctcttcg cgtcagtgtg cggcatttgt ttctggcgtg gtgaaagatg ccttcacctt
     7381 gtggctgaac cagaacgttc aggcagctga actgctggcg gagatggcga tttccagcgc
     7441 ccagcgtcgt atgcgtgcgg ctaaaaaagt ggtgcgtaaa aagctaacca gcggcccggc
     7501 gctgcctggt aaattggctg actgtaccgc gcaggatctt aaccgtaccg aactattcct
     7561 tgtggaaggt gactccgcag gcggatctgc caagcaggcg cgcgatcgcg aatatcaggc
     7621 gatcatgccg ctgaaaggta agatccttaa cacctgggaa gtctcttccg acgaagtgct
     7681 ggcttcgcag gaagtgcacg atatttcggt agcgatcggt atcgatcctg acagtgacga
     7741 tctgagccaa cttcgttacg gcaagatctg tatcctggcg gatgctgact ccgatggtct
     7801 gcacattgcc acgctgctct gcgctttgtt tgtaaaacac ttccgcgcat tggtgaaaca
     7861 cggtcacgtt tacgttgcac tgccaccgct ctaccgtatt gacctcggga aagaggttta
     7921 ttacgcgctg acggaagaag agaaagaggg cgtacttgag caattaaaac gcaagaaagg
     7981 caagccaaac gtccagcgtt ttaaaggtct cggggaaatg aacccgatgc aattgcgcga
     8041 aaccacgctt gatccgaaca ctcgccgtct ggtgcagttg actatcgatg atgaagacga
     8101 tcagcgtact gacgcgatga tggatatgct gctggcgaag aaacgctcgg aagatcgccg
     8161 caactggttg caagaaaaag gcgacatggc ggagattgag gtctgataat cttaacccgg
     8221 caaacatgtg ttttgccggg ttgtcgttga ttattttatt gggcacagtt tattacgatc
     8281 tggaaatagt gttgccagcg aaccagccag agttttccag tccgtagcca ttttttctgc
     8341 ttgtgcagtt gttattactg caccttgttg tttaataagc tcatcctcca ctctggtata
     8401 gtgaataatg gtaaaatccg tagaggacca tttatcctta gagaacatct tcgtcatcgc
     8461 atgaatgttt gcttgcccgt catcgataaa aacaatcgcg tcatatttgt tccccatttt
     8521 atccagcata taatcgagaa ttacgccttt atcttgcccg gaagaaaaaa ataccccctg
     8581 gctgtacagc catgaccgtt tgagtttccc tttttcaaag ggtggcaaag tatcctcttt
     8641 cttcctgagt gcggaagacg aaaaatcaat gccgtttcgc tttaattccc gcaatgtcgg
     8701 aaagagagta tccggtgcac gggatgtcag agcaaaagcg gtatgctttt gttgccactg
     8761 ttttaacatt gtcggtaact gtggctcggt aagctgcata gggctaagtt cgtaaagcat
     8821 actgattgta ttttcataca ggcaaggaac tttttgcgag tcatccggct ttaaaggcaa
     8881 tttatcggtt tgccattgat accagatatc accgccaatg cgagtgccac tcgttaataa
     8941 cgtgttatca atgtcaaaaa ccaataatat cttatcggat ggat
//
LOCUS       DAZKJR010000184         8721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKJR010000184 DAZKJR010000000
VERSION     DAZKJR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.185"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..1220
                     /gene="ompN"
                     /locus_tag="ACHOWS_004608"
     CDS             87..1220
                     /gene="ompN"
                     /locus_tag="ACHOWS_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFQ0050081.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1361..1795
                     /gene="uspF"
                     /locus_tag="ACHOWS_004609"
     CDS             1361..1795
                     /gene="uspF"
                     /locus_tag="ACHOWS_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFQ0050082.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1971..2906
                     /gene="ttcA"
                     /locus_tag="ACHOWS_004610"
     CDS             1971..2906
                     /gene="ttcA"
                     /locus_tag="ACHOWS_004610"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFQ0050083.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3035..4408)
                     /gene="dbpA"
                     /locus_tag="ACHOWS_004611"
     CDS             complement(3035..4408)
                     /gene="dbpA"
                     /locus_tag="ACHOWS_004611"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFQ0050084.1"
                     /translation="MSAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPTILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRMLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4438..4611)
                     /gene="ynaL"
                     /locus_tag="ACHOWS_004612"
     CDS             complement(4438..4611)
                     /gene="ynaL"
                     /locus_tag="ACHOWS_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFQ0050085.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4886..5869)
                     /gene="zntB"
                     /locus_tag="ACHOWS_004613"
     CDS             complement(4886..5869)
                     /gene="zntB"
                     /locus_tag="ACHOWS_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFQ0050086.1"
                     /translation="MEAIKGADVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5883..5930)
                     /gene="ydaN"
                     /locus_tag="ACHOWS_004614"
     CDS             complement(5883..5930)
                     /gene="ydaN"
                     /locus_tag="ACHOWS_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFQ0050087.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6124..7356
                     /gene="dgcM"
                     /locus_tag="ACHOWS_004615"
     CDS             6124..7356
                     /gene="dgcM"
                     /locus_tag="ACHOWS_004615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFQ0050088.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7377..7940)
                     /gene="smrA"
                     /locus_tag="ACHOWS_004616"
     CDS             complement(7377..7940)
                     /gene="smrA"
                     /locus_tag="ACHOWS_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFQ0050089.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLTQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8135..>8721)
                     /locus_tag="ACHOWS_004617"
     CDS             complement(8135..>8721)
                     /locus_tag="ACHOWS_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0050090.1"
                     /translation="FQLFSVPFYFWLSKKINLRWLLMAGLGGFVFSMYLFTPITHEWG
                     WQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGGAIGIALCG
                     SILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNRSGSDQTSEILASTKLLSQLM
                     LREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
BASE COUNT         2322 a         2194 c         2034 g         2171 t
ORIGIN      
        1 tcggctaact gaacttctcc tttaggatgc tttcactccc caaaatgggg ataaaaggca
       61 aataaaataa ctaaaggatt tattcaatga aaagcaaagt actggcactt ttaattcctg
      121 ccctgctcgc cgcaggtgct gcacatgcag ccgaagttta taataaagac ggcaacaaat
      181 tagatctgta tggcaaagtt gatggcctgc attatttttc tgataattca gcgaaagatg
      241 gcgaccagag ctatgcgcgt ctgggtttta aaggcgaaac ccaaattaac gatcaactca
      301 ctggctacgg tcaatgggaa tacaatattc aggcaaacaa cactgaatct tcaacaaacc
      361 agtcatggac ccgtctggca tttgccggac tgaaatttgc agattacggt tctttcgatt
      421 acggacgtaa ttatggcgta atgtacgaca tcgaaggctg gaccgatatg ctgcctgaat
      481 ttggcggtga ctcttatacc aatgcagaca actttatgac tggtcgtgcc aacggcgtcg
      541 cgacttatcg taatactgat ttcttcggtc tggtaaatgg tctgaacttc gcggtgcagt
      601 atcaaggtaa caacgaagga gccagtaatg gtcaggaagg caccaacaac ggacgtgatg
      661 ttcgccatga aaacggtgac ggctggggtc tttccacaac atatgattta ggcatgggct
      721 ttagcgctgg tgcggcatac acctcttctg accgcaccaa tgaccaggtt aaccatactg
      781 cggcgggtgg tgataaagca gacgcgtgga ctgctgggct aaaatacgat gctaacaata
      841 tttacctggc aaccatgtat tcagaaacgc gtaacatgac cccgtttggc gacagcgatt
      901 atgcagttgc gaacaaaacc cagaactttg aagttactgc acagtaccag tttgattttg
      961 gtctgcgtcc ggcagtctct ttcctgatgt ctaaaggccg tgacctgcac gctgccggtg
     1021 gtgcagacaa cccggcaggt gttgatgata aagatctggt taaatacgcc gatgttggcg
     1081 cgacttacta tttcaacaaa aatatgtcca cctacgttga ctataaaata aacctgttgg
     1141 atgaagatga cagcttctac gctgccaacg gcatctctac cgatgatatt gtcgctttag
     1201 gtctggttta tcagttctaa atcctcctgc ccgctgttat ggcgggcttt ttctgcttat
     1261 tcttcctcct ttgatctaaa ttaaaaatgt gaactccgtc attacacaaa aagtgtcatc
     1321 tggcgttaca ctttatgcgg atactaaaac aggaggtttt atgaacagaa cgattcttgt
     1381 ccctatcgat atttccgatt cagaattaac tcaacgcgtg attagccacg ttgaggcaga
     1441 ggcaaagatt gatgatgcag aggttcattt cctgacggta ataccttcac tgccctacta
     1501 tgcctctctg ggtttagcgt attccgcaga attaccggca atggatgacc tgaaagcgga
     1561 agccaaatcg caactggaag agatcattaa aaaatttaaa ctgccaaccg acagagtgca
     1621 tgtccatgtt gaggaaggct cgcccaaaga ccgcattctg gaattggcga agaagatccc
     1681 cgctcatatg atcatcattg cttcccatcg accggatatc accacttatc tgctcggttc
     1741 taacgccgca gcggtagtgc gtcacgcaga gtgctccgtg ctggttgtgc gctgacatta
     1801 acgcccgcac attgctgcgg gctttttgat tcatttcgca aatgtgctga cattttcccc
     1861 tctaatccgt accatacacg ccacagtttt tatatcagat ttcttatgct gggcgttccg
     1921 gcatgacgca tactcttctg atgccatata acgaattgag tcgcttttaa atgtcgcaaa
     1981 atcaagaaat tagtaagaaa gaacaataca acctgaacaa attacaaaaa cgtctgcgtc
     2041 gtaacgtggg cgaagccatt gctgacttca atatgattga agaaggcgat cgcatcatgg
     2101 tttgcctctc cgggggtaaa gacagctata ccatgctgga aattctgcgc aatttgcagc
     2161 aaagcgcgcc aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag caaccgggct
     2221 tcccggaaca cgttctgccc gagtatcttg aaacgctggg cgttgagtac aagattgtgg
     2281 aagagaacac ttacggtatc gtgaaagaga agattccgga gggcaaaacc acttgctcac
     2341 tgtgttctcg tcttcgtcgc ggtatccttt atcgcaccgc aacggaactg ggggcgacga
     2401 agatcgcgct gggtcaccat cgtgacgata tcctgcaaac gttgttctta aatatgttct
     2461 acggcggcaa gatgaaaggt atgcctccga aactgatgag cgatgatggc aaacatattg
     2521 tgattcgtcc gctggcctac tgccgcgaga aagatattca gcgctttgcc gatgcaaaag
     2581 cgttcccgat tattccgtgc aacctgtgcg gttcacagcc taacctgcaa cgtcaggtga
     2641 ttgctgacat gttgcgtgac tgggataaac gttatccagg gcgtatcgag acgatgttca
     2701 gcgcgatgca gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc gatttcaaag
     2761 gtattaccca cggttctgaa gtggttaacg ggggtgatct ggcgtttgat cgcgaagaga
     2821 tccctctaca accggcgggt tggcagcctg aagaagatga aaatcagttg gatgagttac
     2881 ggctgaatgt ggttgaagtg aaataacccg gatagcgccc gatgcgcaag cttaccgggc
     2941 tactcttatg gaggccggat aagacgcggc cagcgtcgca tccggcaatc ccgaagaaga
     3001 tgtttactct tgcaccctgc gattcaacat ttcattattt taataaccgc acccggcacg
     3061 tttttccttt aatcttcccg ccctgtaact gtttccatgc tttatgagca acagcctgac
     3121 gcaccgcgac atagacatgc gccggatgca cggcgatttt gccaatatct gcgccatcaa
     3181 gcccgatatc tcctgtcagc gcacctaata catcacccgg gcgcattttg gcttttttcc
     3241 cgccatcgat acataacgtt gccatttctg cttccagcgg cacaatggaa ctattaactg
     3301 gcggcgtttg ccagttaagt tttatctgca acatgtcaga aatgatattg gcccgctgtg
     3361 cttcttccgg ggcacagaaa ctgatcgcca gaccgctatt tcctgcacga gctgtacgac
     3421 cgatgcgatg tacatgaact tcagggtccc acgccagctc aaagttcacc accagctcaa
     3481 gcgattttat atccagacca cgcgcagcaa catcagtcgc gaccagtaca cgggcgctac
     3541 cgttagcaaa acgtaccagg gtctgatcgc gatcgcgttg ctccagatcg ccgtgtaacg
     3601 acaatgcact ttgccctact tcattcagcg cgtcgcagac agactggcaa tcttttttgg
     3661 tattgcaaaa caccacgcaa gaggatggct gatgcaagct taataaccgt tgcaacagag
     3721 gaattttgcc tttgctggat gtctcataaa attgttgttc aataggtggc agagcatctg
     3781 ttgagtcaat ttcaatcgct aaaggatcgc gttgcactct tccgctgatt gcggcgatgg
     3841 cttccggcca ggttgccgaa aacagaagcg tctgtcgaga tgctggcgca aaacggatga
     3901 catcatcaat ggcatcgcta aatcccatat ccagcatgcg gtcggcctca tccatcacca
     3961 gcgtattcaa cgcatccagt gataccgtgc ctttttgcag atgatccagc atacggcccg
     4021 gcgttgccac gataatatgc ggcgcatgtt gcaacgaatc acgctgcata ccgaacggtt
     4081 gaccaccgca caacgtcaaa attttggtat ttggcagaaa acgcgccagc cgacgcaatt
     4141 cacctgccac ctgatccgcc agttcacgcg taggacacag cactaaagcc tgggtttgaa
     4201 atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt ttgccgctgc
     4261 cggttttcgc ctgcacgcga acatcttttc cggcaaggat cgtcggaagc gcggcggcct
     4321 gcaccggcgt catggttaaa taacccaact cattaaggtt cgtgagttgg gcgggaggca
     4381 aaacattcag ggtagaaaaa gcggacacaa tttattctcg tggtcatcga cgcaaagtca
     4441 gcaggcgcgt atcctcgcag atctacgctc acgatgcgac aatttaatcg gttcttcgtc
     4501 gggtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcactg gaacaggatc
     4561 gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca tatttccctc
     4621 cggtcattgg gtggactctt aaagggtaga cgctgacaaa taacaggcaa aaaaaagccg
     4681 actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca aaaaaggaag
     4741 taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc aagagagata
     4801 ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg tttttgtata
     4861 cagacagccg ggaaaattgc ttttgttaca accatttact acgatgcaac cataaagcaa
     4921 caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat tgccacccgc
     4981 cgcctgggat cccaccaagg ttgacgccaa ataaccctgt cagaaaggta ctgggtaaaa
     5041 agaccattgc catcaacgac attgtatagg tacgacgcgc taaattttcc tgcatcacct
     5101 gagcgatttc atcagccatc acgccagtcc gtgctataca ggcgtcgatt tcgtcaaggc
     5161 cgcgcccaag gcgatcggca atatcctgca tacgacggcg ttggtcatcg ctcatccacg
     5221 gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata tagcgacgca
     5281 tcacaattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt tgctgatcaa
     5341 ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat tcgctggaat
     5401 gatcggtcaa cgcatcacac acatccacca gccatccccc gcaatcggtc ggaccggtgc
     5461 cttcttccag atcgcttacc acatcgtcca gcgccagcac tttgcgttgt cgggtcgaaa
     5521 caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg cgttcatcgg
     5581 tgctgccgtt tatacagcgc aatgtaatca gcgtaccttc accgagacgg ctgactcggg
     5641 gccgtgtgct ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc ggtgttgtcg
     5701 ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag gggtgcgctt
     5761 catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc agcatccagg
     5821 caaatactgc atccgggaca ttaacgtccg ctcccttaat cgcttccaca gtgcctccat
     5881 catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat ctcatcaccc
     5941 cattacccta aaatgattaa taaaattctg tctaaattga ataaaaaaag caaaatgctt
     6001 ttccatatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga attatctgat
     6061 catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa taacgggatc
     6121 tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt ctggatcgtt
     6181 tcgccctttg aggaacagtt aatttatgcc aacagcgcgg cgcgactgtt gatgcaagac
     6241 ctcacgttta gtcagctacg aaccggtccc tattccgtct cctcacaaaa agaactgccg
     6301 aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac cgttcagcgc
     6361 aatgaagagg aaacagcatt gagctgtcgg cttgttttgc gaaagctgac agaagcagaa
     6421 ccggtgatta ttttcgaagg tatcgaagca ccggcaacgc tgggtttaaa agccagtcgc
     6481 tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct gactaactct
     6541 gcaccaatgt tgttgattga cccctcacga gatggccaaa tcgtcgatgc taacctcgcc
     6601 gcgctcaatt tctatggcta taaccatgaa acgatgtgcc agaaacatac ctgggaaata
     6661 aatatgctcg ggcgacgcgt catgcctatc atgcatgaaa tttcgcattt acccggtggc
     6721 cataagcctt tgaattttgt tcataaactg gcggatggtt cgactcgtca tgtgcagacc
     6781 tatgccggtc caattgaaat ttatggcgac aagctcatgt tatgtattgt gcatgatatt
     6841 actgagcaaa aacggctgga ggagcagctg gaacatgctg cccaccatga tgcgatgacc
     6901 ggattactga atcggcgaca gttttatcac attaccgagc caggccaaat gcagcacctc
     6961 gccatcgctc aggattacag cttgttgctc atcgacaccg atcgttttaa acacattaac
     7021 gatctctatg ggcattctaa aggtgatgag gtgttatgcg cccttgcccg cactctcgaa
     7081 agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt tgtcttattg
     7141 ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg cgtaagcgtg
     7201 gcaaaagtga gtatttcggg cttaccacgc tttactgtca gcattggtgt ggcgcaccac
     7261 gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt gtatcgagcg
     7321 aaaaatgatg gacgcaaccg cgtgctggcg gcataagcca cggatgcgtc tcgtgatcaa
     7381 cgactgcgct tagcgtggcg ctcccagttt tcttgcttcg cctgcgctgt tttacgtagt
     7441 gcgacgtaac acgccccgct gccgccatga tgcggtagcg cagtgcaata tgcctgaaca
     7501 tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc atgcgattta
     7561 tcatcccgtc ctttgccatg aataatcagc acgttacgca aaccatccgc cagggcttgt
     7621 tgaatgaaac tgaacaccat tttgcggcac tcttccaccg gctggcgcaa aagattcagg
     7681 ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg ttgcaaccct
     7741 tcccgccgaa actccagcgg ctgagttagc gggatgatgt cgagaaatcc ggtggtgagg
     7801 aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg cgttggatgc
     7861 cagtggacat ctgtagcacg tttcagcggc tggacatctt ccatggcgtc aagaaacagc
     7921 gatttgtcgt caaggttcat gtaacctcct atgcaccttc acacgtcgtg attcagacgg
     7981 gttaattgtt catacttact gcatcgttcc cttcacagat tgaaacaata ctctacttca
     8041 tacctgtgag tatctgcgca ggcggggcat tttatactag cctgttagca gtatcgaaca
     8101 gtttagcccg agtttaattc agcacaggct ccgatcagga tgatttattc atagcaggaa
     8161 ccaacaaaaa tgcgataaat aacagaccac tgattagcag aaaggtatcc gaaaatgcca
     8221 tagtttgcgc ctccctcagc atcaactgac tcaataattt ggttgaagcc agtatttccg
     8281 atgtctggtc acttccactg cgattaaaaa acaaagcact tctggaaatg aagtcattca
     8341 ccgtatgggg aacagaaacc attttttcac ccattctcga aaaatgaaaa ttagttcgat
     8401 tattgagaat agagccacac agggcaatac caatcgcgcc cccaagatta cgtgttaaat
     8461 taaatacacc agaggctaac ttcagccgtt cctttggaat tccccccagc gttaatgtta
     8521 cgatgggggc catcgcaaac tgctgcgaaa tccctctaat tgcctgtgga aacagtaact
     8581 cctgccatcc ccattcgtgg gttatagggg taaaaagata cattgaaaac acaaatccac
     8641 ccagccccgc catcaacagc catcgcagat ttattttttt acttaaccag aagtaaaagg
     8701 gaacagaaaa taactggaaa a
//
LOCUS       DAZKJR010000185         8693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKJR010000185 DAZKJR010000000
VERSION     DAZKJR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.186"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(226..1158)
                     /locus_tag="ACHOWS_004618"
     CDS             complement(226..1158)
                     /locus_tag="ACHOWS_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0050091.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKTIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1452..2207
                     /gene="mlaA"
                     /locus_tag="ACHOWS_004619"
     CDS             1452..2207
                     /gene="mlaA"
                     /locus_tag="ACHOWS_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFQ0050092.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2269..3609)
                     /gene="fadL"
                     /locus_tag="ACHOWS_004620"
     CDS             complement(2269..3609)
                     /gene="fadL"
                     /locus_tag="ACHOWS_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFQ0050093.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            3981..4265
                     /locus_tag="ACHOWS_004621"
     CDS             3981..4265
                     /locus_tag="ACHOWS_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFQ0050094.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            4446..5756
                     /gene="fadI"
                     /locus_tag="ACHOWS_004622"
     CDS             4446..5756
                     /gene="fadI"
                     /locus_tag="ACHOWS_004622"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFQ0050095.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLLDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEMDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            5756..7900
                     /gene="fadJ"
                     /locus_tag="ACHOWS_004623"
     CDS             5756..7900
                     /gene="fadJ"
                     /locus_tag="ACHOWS_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFQ0050096.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSGGYDAEARAFGEL
                     VMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            8103..8588
                     /gene="sixA"
                     /locus_tag="ACHOWS_004624"
     CDS             8103..8588
                     /gene="sixA"
                     /locus_tag="ACHOWS_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFQ0050097.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2094 a         2094 c         2301 g         2204 t
ORIGIN      
        1 taacagaaat ggccccgtac ggaagtgcgg ggcatgctta acgggatgtg tacataaatg
       61 ggtacggata tgacaattat tttggtattt tctatattta aatcaatgaa ttaatgctta
      121 tattcattat atccatttaa ctaagaggac aatgcggcat gagtataccc gctaatggac
      181 tgcggggtaa gtacgctgcc gctcgattgc ttaaaccctc gccatttatg ccgggttttt
      241 atcatttttc ttaatgtttt ccgcacgttc tgctttttgg cgtgcttctg ctttgcgctt
      301 gttgctcatg tcgttacgga tctgtgcatg actcattaac gcgaagataa aggtgccgcc
      361 gcagatgttc cccgctaaag taggtagtgc gaagggccag atgaaatcgc tccagtgcag
      421 cgtaccgtta aacaccagat agaggatttc aacagaaccg acaacgatgt gagtggtgtc
      481 acccagggca ataagccagg tcatcaatat aatcaccaca atctttgccg cacccgctgc
      541 aggaaacatc caaaccatag tggcgatcag ccagccggaa atgatcgcgt tggcaaacat
      601 ctcgctgggg gtgttcttca tcacatccat gccgattttg acaaatgcat cgcgagtttc
      661 ttcattgaag attggcatat attcaaatgc ccacgccgca atacctgtcc cgagaatatt
      721 acccagcagc acgacgcccc ataaccgcat aagtaagcca acgttgctca ttgtcggttt
      781 ttgcatgacg ggtagtaccg cagtcacggt gttttcggta aataattgct ggcgggccat
      841 aatgacgata ataaaaccaa aggtataacc gagattctcc agcaagaagc tgcccggcac
      901 gccttccagt tcgacatgaa atatcccttt tgccagtagc gaagcgccca tcgacagacc
      961 cgccgcaatg gctgaccaca gtagtgccat tgcgtcgcgt tccagctctt tttcaccatc
     1021 ctggcggata tgctcatgaa ttgccatcgc ccgggagggg agtcgatctt catctatttc
     1081 tattgttttg ccgcgctctt tttcttcgct ctcaacttca atttcgtcgc tgtgttgatc
     1141 aattttgtcg ttgtccatgg tctcttctct tgaattagca cgtatagcta aagcgtagcg
     1201 gcttttttgc tcgcaactgg cgggagttac tctgaaaatg tagaaaaggc tgcgtttgcc
     1261 tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa
     1321 atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa
     1381 acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca
     1441 gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg
     1501 ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgctagaa gggttcaacc
     1561 gcaccatgta caacttcaat ttcaatgtat tagacccgta tattgttcga ccggtcgctg
     1621 tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaacgg tttgagcaac tttactggca
     1681 accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga
     1741 tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc tttattgatg
     1801 ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc
     1861 ttggtcatta tggtgtgggt tatgggccat acgttcagtt accgttctac ggtagcttca
     1921 cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga
     1981 cctggccgat gtctgtgggt aaatggacgc ttgaagggat agaaactcgt gcgcagttgt
     2041 tggattccga tggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt
     2101 acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga
     2161 acgcgcaagc gattcaggat gatttaaaag atatcgattc tgaataagaa acaaacaaaa
     2221 aaggtgagtc gcaaaactca ccttttttct tattaagcga acgcgttatc agaacgcgta
     2281 gttaaagtta gtaccgaaca gccaggcttt accttcagac tcgaactggt atgggccttc
     2341 gttaattttc acgctctgac cgtgcatata agaaacacca acatcgactg aagcatcttt
     2401 attaaatgcg taagtcgtac ctgcactcag ccagaaacgg tcctggtccg gaatggagat
     2461 agaacgattc tgtgccggaa ctgggctgtc atcaaaggcg ataccggtac ggaaggtcca
     2521 gttatcatcg tagtaataag tggttcccaa cgcgatgcgg taagcatctt taaagccttc
     2581 atgtttctgg aacagcgtgt cgccactcgt tgaggtcgct ttcagctgct ggaactgact
     2641 ccagctggtg taagccaggc tatagtgaat tgcccactgc ggatcaacac ggttataacc
     2701 cgacacttcc cacatttcag gcaggttcag cgtcagataa cccgactgcg ttgcgccacc
     2761 tgtcgcggta ggaattggca aaccgtagtt attaaaagcc gggttaagat cgctgctgta
     2821 gttacccttg aagtcaattt tcacttcaga gcggtaggtc aatgcgtagc ggttgttttt
     2881 atccagttca tacaggatac cggcgttcca gccaaagccc cactggttac cgttcaggtg
     2941 agcgattttg gtattactgt cgatgccgtt ggcggtagct gctaatgctt gcccctgagg
     3001 agtcagccaa gcaggagatt gcctaatctg accagccacc agttgcccca gatcgcctgc
     3061 gaaacgttca attttcgcgc gagcgtagac ggcgttgaaa ccaagaccaa agctccatgc
     3121 attatttaag cgatatgcac cgcttaagtt caggttcatg gtttcgaggt cggtggtacc
     3181 accgacagaa ccgcctgcat aagtatcgtt aaactctgtc gccagaccat agttagaggt
     3241 aattgaagcc ccccaaccaa attggtcgtt aatcggtgca acaaagtgca tgttcggaac
     3301 ccatgccgta ggcgcgatgt tatcggcttt caggctacga ccagacggag acgttccgct
     3361 gatatttaca tccggatcaa tataaaccgc acccgcagaa aatgtcgggc ggtcaaacat
     3421 ggtaatcaat gcggggttac ggctaacgtt acctgcatca tcggcaattg caccttcccc
     3481 tgaataagcc cggcccaggc cagaggaaga aaattcgttt aactgaaagc ctgccgacca
     3541 ggcctgggtg gagataagtg ccactgcgac tgcgagagca gactttgtaa acagggtttt
     3601 ctggctcatg accataacct caatgattta tttttataca aactatgtta cgtgctgtaa
     3661 caggagcgcg aagtgtaggg tctgaggtac atcttagaaa tcagaccagt ggcgagagta
     3721 taggtcggac cagctggaat gttgcaagta tgtttctatc ttttttcaga tatgatctaa
     3781 gaaacgcgat ccagctggca aaattgggag gtggcgcact catggattcg ggtgaatttt
     3841 gttttagatc atttttaagt gtgatttcgg tcacttatcc gattttgcag aattaacaac
     3901 tggagcagca ctttccggaa gcgtaaaata tccacaacgt ttatctggca ccttgggtgc
     3961 acatacagag gaaatcaacg atgagtaaat gtagtgctga tgaaaccccg gtttgctgct
     4021 gtatggatgt tggcaccatt atggacaact ccgactgcac cgcgtcttac agccgtgtgt
     4081 ttgcaaaccg cgcagaagca gaacaaacgc tggcggcgct gactgaaaaa gcccgtagcg
     4141 tggaatccga accgtgcaaa atcaccccga ctttcactga agagtctgac ggtgtgcgcc
     4201 tggatatcga tttcactttt gcctgcgaag ctgaaatgct gatcttccag ctgggtctgc
     4261 gttaattcct ctcgggtaca tacgcccctg tcagttgctg gcaggggcgt tttcttttct
     4321 cttctacttt tctctctgtg tcatagctcc cactttccct tttctccact tggctaaatg
     4381 taaaaaaatg gttaagacta tgatcaggtc agaccacttt atttattatt ttacagggga
     4441 gtgttatggg tcaggtttta ccgctggtta cccgccaggg cgatcgtatc gccattgtta
     4501 gcggtttacg tacgcctttt gcccgccagg cgacggcttt tcatggcatt cctgctgttg
     4561 atttggggaa gatggtggta ggcgaactgc tggcacgcag cgagatcccc gccgaagtga
     4621 ttgaacaact ggtttttggt caggtcgtac aaatgcctga agcccccaac attgcgcgtg
     4681 aaattgttct cggtaccgga atgaatgtgc ataccgatgc ttacagcgtc agccgcgctt
     4741 gcgctaccag tttccaggcg attgcaaacg tcgcagaaag cctgatggcg ggaactattc
     4801 gcgcggggat tgccggtggg gccgattcct cttccgtatt gccaattggc gtcagtaaaa
     4861 aactggcgcg cgtgctggtt gatgtcaaca aagctcgaac catgagccag cgcctgaaac
     4921 tcttctctcg cctgcgtttg cgcgacttaa tgccggtgcc tccggcagtg gcggaatact
     4981 ctactggctt gcggatgggc gacaccgctg aacaaatggc gaaaacctac ggtattactc
     5041 gcgaacagca agatgcatta gcgcaccgtt cgcatcagcg agccgctcag gcgtggtcag
     5101 acggtaaact caaagaagag gtgatgactg cctttatccc tccttataaa caaccgcttg
     5161 ccgaagacaa caatattcgc ggtaattcct cgcttgctga ttacgcaaag ttgcgtccgg
     5221 cgtttgatcg taaacacggg acggtaacgg cagcaaacag tacgccgctg accgatggcg
     5281 cagcggcggt gatcctgatg accgaatcgc gggcgaaaga attagggctg gtaccgctgg
     5341 ggtatctgcg cagttacgca tttactgcga tagatgtctg gcaggacatg ttgctcggtc
     5401 cggcctggtc aacgccgctg gcgctggaac gtgccggttt gacaatgagc gatctgacat
     5461 tgctcgatat gcacgaagcc tttgcagctc agacactggc gaatattcag ttgctgggta
     5521 gcgaacgttt tgctcgtgat gtactggggc gtgcacatgc cactggcgaa atggacgata
     5581 gcaaatttaa cgtgcttggc ggttcgattg cttacgggca tcccttcgcg gcgaccggcg
     5641 cgcggatgat tacccagaca ttgcatgaac ttcgccgtcg cggcggtgga tttggtttag
     5701 tgaccgcctg tgctgcgggt gggcttggcg cggcaatggt tgtggaggcg gaataatgga
     5761 aatggcatca gcgtttaccc ttaatgtccg tctggacaac attgctgtta tcaccatcga
     5821 cgtaccgggt gagaaaatga ataccctgaa ggcggagttt gcctcgcagg tgcgcgccat
     5881 tattaagcaa ctccgtgaaa acaaagagtt gcgcggcgtg gtgtttatct ccgcgaaacc
     5941 agacaacttc attgctggcg cagacatcaa catgatcgcc aactgcaaaa cggcgcaaga
     6001 agcggaagca ctggcgcggc agggccaaca gttgatggcg gagattcatg ctttgcccgt
     6061 tccggttatc gcggctattc atggcgcttg cctgggcggt gggctggagt tggcgctggc
     6121 gtgccacggt cgcatgtgta ctgacgatcc taaaacggtg ctcggtttgc ctgaagtaca
     6181 acttggatta ttacccggtt caggcggcac ccagcgttta ccgcgcctga taggtgtcag
     6241 cacagcattg gagatgatcc tcactggaaa acaacttcgg gcgaaacagg cattaaagct
     6301 ggggctggtg gatgacgtcg ttccgcaatc cattttactg gaagccgctg ttgagctggc
     6361 aaagcaggat cgcccatctt cacgccctct acctgtacgc gagcgtattc tggcggggcc
     6421 gttaggtcgt gcgctgctgt tcaaaatggt cggtaagaaa acagaacaca aaactcaagg
     6481 taactatccg gcgacagaac gcatcctgga ggttgtcgaa acgggattag cgcagggcac
     6541 cagtggcggc tatgacgctg aagcccgtgc ttttggcgaa ctggtgatga cgccgcaatc
     6601 gcaggcgctg cgtagtatct tttttgccag tacggacgtg aagaaagatc ctggcagtga
     6661 tgcgccgcct gcgccattaa acagcgtggg aattttaggt ggtggcttga tgggcggcgg
     6721 tattgcttat gtcactgctt gtaaagcggg gcttccggtc agaattaaag atatcaaccc
     6781 gcagggcata aatcatgcgc tgaagtacag ttgggatcag ctggagggca aagttcgccg
     6841 tcgtcatctc aaagccagcg aacgtgacag acagctggca ttaatctccg gaacgacgga
     6901 ctattgcggc tttgcccatc gcgatctgat tattgaagcg gtgtttgaaa atctcgaatt
     6961 aaaacaacaa atggtggcgg aagttgagca aaattgcgcc gctcatacca tctttgcttc
     7021 gaacacgtca tctttaccga ttggcgatat cgccgctcac gccgcgcgac ctgagcaagt
     7081 tattggcctg catttcttca gtccggtgga aaaaatgccg ttggtagaaa ttattcccca
     7141 tgccggtaca tcggcgcaaa ccatcgccac cacagtaaaa ctggcgaaaa aacagggcaa
     7201 aacgccaatt gtcgtgcgtg acaaagccgg gttttacgtc aatcgcatct tagcgcctta
     7261 cattaatgaa gctatccgca tgttgaccga aggtgaacgg gtagagcaca ttgataccgc
     7321 gctggtgaaa tttggttttc cggtaggccc aatccaactt ttggatgagg taggaatcga
     7381 caccgggact aaaattattc ctgtactgga agccgcttat ggagaacgtt ttagcgcgcc
     7441 tgcaaatgtt gtttcttcaa ttttgaacga cgatcgcaaa ggcagaaaaa atggccgggg
     7501 tttctatctt tatggtcaga aagggcgtaa aagcaaaaaa caggtcgatc ccgccattta
     7561 cccgctcatt ggcacacaag ggcaggggcg actctccgca ccgcaggtag ctgaacggtg
     7621 tgtgatgttg atgctgaatg aagcagtacg ttgtctggac gagcaggtta tccgtagcgt
     7681 gcgtgacgga gatattggcg cggtatttgg cattggtttt ccgccatttc tcggtggtcc
     7741 gttccgctac atcgattctc tcggtgcggg tgaagtggtt gcaataatgc aacgactggc
     7801 cacgcagtat ggttcccgtt ttaccccttg cgagcgttta gtcgagatga gcaagcgtgg
     7861 ggaaagtttt tggaaaacaa ctgcaactga cctgcaataa gaaggtcaaa gctatatgaa
     7921 tccgcgctga atggcggagt gttgctcaaa atgtaaacgc atattgacta tacttacgcc
     7981 attgaggtaa aaaacagcgt ttcattcggt gaatggataa ggcacaatgc cggccaccgt
     8041 tttctttctc tggtttcaga tgaaagaaaa cgggcgaatc tggttaacaa aagcggtgca
     8101 atatgcaagt ttttatcatg cgtcacggcg acgcagccct cgatgccgcc agtgattcgg
     8161 ttcgtcctct gaccactaac ggttgtgacg aatctcgcct gatggcgaac tggctgaaag
     8221 gtcaaaaagt ggaaatcgaa cgcgttctgg tgagcccgtt cctgcgagcc gagcaaacac
     8281 ttgaagaagt aggggattgt ctgaacctgc cttccagtgc agaagttctg ccggagttaa
     8341 cgccctgcgg cgatgttggt ctggtcagtg cctatttgca ggcgttgacc aatgaaggtg
     8401 ttgcttcggt actggtgatc tcccacttac ccttagtcgg gtatctggtt gccgagttat
     8461 gtccgggcga aacgccgcct atgtttacca cctcggccat tgccagcgta acgcttgatg
     8521 aaagcggaaa cggcacattc aactggcaga tgagcccgtg taatctgaag atggcaaaag
     8581 cgatttaata cgcatgcaca aggcccgctg gtgtaatcta tcgggccttt atttttttct
     8641 ggcaatgttg aatttatgca atcttattgt actaacaatg atccgcttgt gat
//
LOCUS       DAZKJR010000186         8673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKJR010000186 DAZKJR010000000
VERSION     DAZKJR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.187"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(328..534)
                     /locus_tag="ACHOWS_004625"
     CDS             complement(328..534)
                     /locus_tag="ACHOWS_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFQ0050098.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            847..936
                     /gene="kdpF"
                     /locus_tag="ACHOWS_004626"
     CDS             847..936
                     /gene="kdpF"
                     /locus_tag="ACHOWS_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFQ0050099.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            936..2609
                     /gene="kdpA"
                     /locus_tag="ACHOWS_004627"
     CDS             936..2609
                     /gene="kdpA"
                     /locus_tag="ACHOWS_004627"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFQ0050100.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFIICTGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLALMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2632..4680
                     /gene="kdpB"
                     /locus_tag="ACHOWS_004628"
     CDS             2632..4680
                     /gene="kdpB"
                     /locus_tag="ACHOWS_004628"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFQ0050101.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPVDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4689..5261
                     /gene="kdpC"
                     /locus_tag="ACHOWS_004629"
     CDS             4689..5261
                     /gene="kdpC"
                     /locus_tag="ACHOWS_004629"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFQ0050102.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5254..7938
                     /gene="kdpD"
                     /locus_tag="ACHOWS_004630"
     CDS             5254..7938
                     /gene="kdpD"
                     /locus_tag="ACHOWS_004630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFQ0050103.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7935..8612
                     /gene="kdpE"
                     /locus_tag="ACHOWS_004631"
     CDS             7935..8612
                     /gene="kdpE"
                     /locus_tag="ACHOWS_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFQ0050104.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1815 a         2249 c         2510 g         2099 t
ORIGIN      
        1 atacgtaccc cggctgaacc ctgaacaatg ctaccgccat actgcgtcat gtcgccctga
       61 cgcgccgccg gttttccgct catctgctca tccttgtttt atcgggttat ttgtctgctg
      121 acaggcgaaa ccctacagat ttataagaaa gccttaagtg agatctctgt cagataagaa
      181 caattaggaa aatatttata tatatttcat gaatatatgt ttcgattggt ataacggtgt
      241 ctaaatattc ctctgaacat cttcctgata caaaaaaaac cgccagcgtt tccgctggcg
      301 gttttcaatg tattcagaac tcagcaatca gtaaaaatca ccagtcgcct tttcggcctg
      361 atccatccac accggtttat cgctggtttt cgaccagacg cggtgcaggt agctgtaaaa
      421 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
      481 ataagtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
      541 ctcccccttt tgtatctacc cggtgaatag caccggaaaa atgaatttgt ttatctgatg
      601 aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt
      661 gatggtttat ttacattcat accgtaatta agttacacaa aggttaaatt aatactaaac
      721 attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
      781 ttgcgaaatc tttgcagcca gaattctact cttccggtat cacttttagg ccactggagg
      841 tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg tgtttttatt actgggttat
      901 ctggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
      961 ttgccacgtt tttactggtg ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
     1021 ggctgattaa tgacattcct cttcccggta cgacgggcgt tgagcgcgta ctttttcgcg
     1081 cgcttggcgt ctctgaccgc gagatgaact ggaagcagta tctctcagcc attcttggcc
     1141 tgaatatact ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
     1201 ttaatccaca gcagttgcca ggactgtcgt gggatctggc gctgaatacc gccgtcagct
     1261 ttgtcacaaa taccaactgg caatcttaca gcggtgaaac cacgttgagc tatttcagcc
     1321 agatggcggg cttaacggtg caaaactttc tttctgccgc cagcgggatt gcggtgattt
     1381 ttgccctcat acgtgcattt acccgccaga gcatgagcac actcgggaat gcctgggtcg
     1441 atctgctacg catcacgtta tgggtgctag tccctgtggc gttgttgatc gcattgtttt
     1501 ttattcaaca aggtgcgctg caaaactttc tgccctatca ggcggtgaat accgttgaag
     1561 gagcgcaaca gctgttaccc atggggcctg tagcttctca ggaagcgatc aagatgctcg
     1621 gcactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
     1681 cactgaccaa cttcgtgcag atgctggcga tcttcttgat cccaacagcg ctgtgctttg
     1741 cctttggcga agtggcgggc gatcgccgtc aggggcgcat gttgctgtgg gcgatgtcag
     1801 tgatttttat catctgcaca ggcgtggtga tgtgggcaga agttcagggt aatccgcatc
     1861 tgctggcact gggcgcggac agcagcatca atatggaagg taaagagagt cgtttcggcg
     1921 tgctggtcag tagcctgttt gcggtcgtga ctacggcggc ttcctgtggc gcggtgattg
     1981 cgatgcatga ttcgtttacc gctcttggtg gcatggtgcc gatgtggctg atgcaaattg
     2041 gtgaagtggt gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc
     2101 tggcggtgtt tattgctggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa
     2161 tcgacgtacg cgagatgaaa ctgaccgcgc tggcgattct ggtcacccca acgctggcgc
     2221 tgatgggcgc ggcgctggca atgatgaccg acgccggacg tagcgccatg ctcaaccctg
     2281 gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcatc cgccgctaac aacaacggca
     2341 gcgcctttgc cggattaagc gccaactctc cattctggaa ctgtttgctg gcgttctgca
     2401 tgtttgtcgg tcgcttcggg gtaattatcc cggtgatggc aattgccggt tcgctggtga
     2461 gtaaaaagag ccagcccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg
     2521 gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc
     2581 ttggtccggt ggcggaatat ctctcctgat gatattgagt gagcactgaa tatgagtcgt
     2641 aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa
     2701 aaattaaacc cgcaggcgca atggcgtaat ccagtgatgt ttatcgtctg gatcggcagt
     2761 ctgttaacca cctttattag cattgcaatg gcaagcggcg cgatgcctgg caatgcgctg
     2821 tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag
     2881 gcgctggcag aaggccgcag taaagcgcaa gccaatagtc tgaaaggggt gaaaaaaact
     2941 gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcccgca
     3001 gatcaactgc gtaaaggcga tatcgtactg gtagaagctg gcgatattat cccctgcgat
     3061 ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggct
     3121 ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ctggcggcac gcgtattctt
     3181 tctgactggc tggtgattga gtgcagcgtt aaccccggcg agacatttct ggatcggatg
     3241 atcgcgatgg tggaaggcgc gcagcgacgc aaaacgccga acgagattgc cctgaccatc
     3301 ctgctgattg ccctgactat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc
     3361 gcatggggtg gtaatgcagt cagcgtaacg gtactggtgg cgctgttggt ctgtctgatc
     3421 ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag ccggatgttg
     3481 ggcgcgaatg tgattgccac cagcgggcgt gcagttgaag cggcgggcga cgtggacgtc
     3541 ctgctgctgg ataaaactgg caccatcacc ctcggtaacc gtcaggcatc agagtttatc
     3601 cctgcgcagg gcgtagagga aaaaacgctg gccgacgcag cacaactggc ttcgctggcc
     3661 gacgaaacgc cggaaggccg cagtattgtg atcctcgcca agcagcgctt taacctgcgc
     3721 gagcgcgatg tgcagtcgct tcacgccact tttgtaccgt ttactgcgca aagtcgaatg
     3781 agcgggatca atatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc
     3841 catgttgagg ctaacggtgg tcacttccct gtcgatgttg atcaaaaagt cgatcaggtt
     3901 gcgcgtcagg gggccacgcc gctggtggtg gtggaaggtt ctcgtgtgct gggcgttatt
     3961 gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg
     4021 ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc cattgctgcg
     4081 gaagcgggtg tcgatgattt tctcgccgaa gcgacaccgg aggccaagct ggcgttaatt
     4141 cgtcagtatc aggcggaagg ccgtttggtg gcgatgaccg gcgacggcac caatgatgct
     4201 ccggcgctgg cgcaggcaga tgtcgcggta gcgatgaact ccggtactca ggcggcgaaa
     4261 gaagcgggca atatggtgga tctcgactct aacccgacca agttgatcga ggtggtgcat
     4321 attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat tgccaacgat
     4381 gtggcgaaat acttcgccat tattccggcg gcgttcgcgg caacgtatcc gcagttaaat
     4441 gcgctgaaca tcatgcgcct gcattcgccc gactccgcca ttctcagtgc ggtcattttc
     4501 aacgccttga ttatcgtctt tttgattccc ctggcgttaa aaggcgtgag ttataaaccg
     4561 cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg
     4621 gtgccgttta ttggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga
     4681 ggtttaccat gcgtggatta cgtccggcat tatcaacatt tctctttttg ttactgatta
     4741 ctggcggtgt ttacccgctg ctgaccaccg cgctggggca atggtggttt ccctggcagg
     4801 ccaacggttc gttgattcgc gaaggtgata cggtgcgcgg ctcggcattg atcgggcaga
     4861 attttactgg caacggctat tttcatggtc gcccgtcggc aacggcagaa atgccctata
     4921 atccacaggc ttctggcggg agcaatctgg cggttagtaa cccggagctg gataaacaaa
     4981 tcgctgcacg cgttgccgca ttacgggccg ctaacccgga tgccagcgcg aacgttccgg
     5041 ttgaactggt gacggcgtcg gcaagcgggc tggacaataa catcaccccg caagcggcgg
     5101 cctggcagat cccacgcgtg gcgaaagcgc gtaatctcag cgttgaacag ctcacgcagc
     5161 tgatcgcaaa atatagccaa caaccgctgg tgaaatatat cggccagccg gtcgtcaaca
     5221 ttgtcgaact caatctggcg ctggataagc ttgatgaata acgaaccctt acgtcccgac
     5281 cccgatcgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa agttttcttc
     5341 ggtgcctgcg caggcgtcgg caagacctgg gcaatgctgg cagaagccca gcgactgcgg
     5401 gcgcaagggc tggatattgt ggttggcgtg gtagaaaccc acgggcggaa agataccgcc
     5461 gcaatgctgg aagggctggc tgttctgccg ttaaaacgcc aggcgtaccg tgggcggcac
     5521 atcagcgagt ttgatctcga tgccgccctc gcccgccgtc cggcactgat cttaatggac
     5581 gaactggcgc acagtaatgc gccaggttct cgtcatccca aacgctggca ggacatcgaa
     5641 gagctgctgg aagctggcat tgatgttttc actaccgtca acgttcaaca tctggaaagc
     5701 ctgaatgatg tggtcagcgg cgtcaccgga attcaggttc gggaaaccgt gcccgatcct
     5761 ttttttgatg ccgccgacga cgtagtgctg gtggacttgc ccccggacga tctgcgccag
     5821 cgactgaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat tgaacatttt
     5881 ttccgcaaag gcaatctgat cgccctgcgc gaactggcac tgcgccgtac tgccgatcgc
     5941 gtagatgagc aaatgcgcgc ctggcggggg catcctggcg aagagaaagt ctggcacacg
     6001 cgcgacgcga tccttttatg catcggccat aacactggta gtgagaaact ggtccgcgcg
     6061 gcggcgcggc tggcgtcacg gctgggtagc gtctggcacg cggtgtatgt tgaaacccct
     6121 gccctgcatc gcttaccgga gaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg
     6181 caggagctgg gcgcggagac ggcaacactt tctgatccag cggaagagaa agcggtagtg
     6241 cgttacgccc gtgagcataa tctcggcaag attattctcg gtcgcccagc ctcgcgccgc
     6301 tggtggcgtc gggaaacgtt tgctgaccga ctggcgcgca tcgcccccga tctcgatcag
     6361 gtgctggtcg cgcttgatga accacccgcc cgcacgatta acaacgcgcc ggatagccgc
     6421 tcttttaaag acaagtggcg ggtacagatt cagggatgcg tggttgccgc cgcgttatgc
     6481 gccgttatca ccttaattgc catgcagtgg ctgatggcgt ttgatgccgc caacctggtg
     6541 atgctgtatc tacttggcgt ggtggtggtg gcgctatttt atggacgctg gccttcggtg
     6601 gttgctaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc acgcggcacg
     6661 ctcgccgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac cgtcgggctg
     6721 gtgatcggga accttactgc tggcgtgcgt tatcaggcgc gggtagcccg ttaccgcgaa
     6781 caacgcacac ggcacttata tgaaatgtcc aaagctctgg ctgtgggccg cagttcgcag
     6841 gatatcgccg ccaccagcga acagtttatt gcctccacgt ttcatgcccg tagccaggtg
     6901 ttgctgcctg atgacaacgg taaattgcag ccgttaacac atccgcaagg aatgacgccg
     6961 tgggacgatg ccatcgcgca gtggagtttt gataaaggtc tgcctgcggg cgcgggcacc
     7021 gacacgttac ccggtgtacc gtaccagatt ttgccgctaa aaagcggcga gaaaacctac
     7081 ggactggtgg tggtggaacc ggggaatctg cgccagttga tgatcccgga acagcagcgc
     7141 ctgctggaga cgtttacgct gttagtcgcc aatgcgcttg agcggctgac gctgaccgcc
     7201 agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc tctgctggcg
     7261 gcgctttcgc atgatttacg cacgccgctc acggtgctgt tcggtcaggc agaaatctta
     7321 acgctcgatc tggcaagcga aggatcgccc cacgcccgcc aggccagcga gattcgtcag
     7381 catgtgctga acacgacccg actggtgaat aatctgctcg atatggcgcg aattcagtcc
     7441 ggcggcttta atttgaagaa agagtggtta acgctggaag aagtggtcgg cagcgcgctg
     7501 caaatgctgg aaccgggttt atcgtcgcct atcaatcttt ctctgccaga accgctgacc
     7561 ttaatccacg ttgacgggcc gctctttgaa cgggtgctga ttaatctgct ggagaacgcg
     7621 gtgaaatacg cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga gggcgagaag
     7681 ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga gcagacgata
     7741 tttgataagt ttgctcgcgg gaataaagag tcggcggtac cgggtgtagg gcttgggctg
     7801 gcaatttgtc gggcgattgt ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg
     7861 gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ccgctcctga acttgaagaa
     7921 tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct attcgtcgct
     7981 ttctgcgcac ggcgctggaa ggcgacggta tgcgcgtctt tgaggccgaa acgctgcaac
     8041 gcggcttgct ggaagcggct acccgtaagc cagatttgat tattctcgat ctcggcctgc
     8101 ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcgcg gtgccggtga
     8161 ttgtgctttc cgcacgtagc gaagagagcg acaaaatcgc cgcgctggat gccggagcgg
     8221 atgattatct gagtaagccg tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt
     8281 tacgtcgcca ctccgccacc gccgcgcccg atccgctggt gaaattttcc gatgttaccg
     8341 tcgatttagc agctcgtgtg atccatcggg gcgaggaaga ggtgcatctc accccgattg
     8401 agttccgcct gctggcagtg ttgcttaata atgcgggaaa agtgctcacc cagcgccagc
     8461 tccttaacca ggtgtggggg ccaaacgcgg tcgaacacag tcattatttg cgtatttata
     8521 tggggcatct gcgacaaaaa ctggaacagg acccggcccg gcctcgccat ttcattactg
     8581 aaaccggtat tgggtatcgg tttatgcttt gaatattaat ttcaatacat cctgcctttt
     8641 attaattaaa gccgtaataa taaatacggc ttt
//
LOCUS       DAZKJR010000187         8481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKJR010000187 DAZKJR010000000
VERSION     DAZKJR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.188"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            217..1347
                     /gene="ompC"
                     /locus_tag="ACHOWS_004632"
     CDS             217..1347
                     /gene="ompC"
                     /locus_tag="ACHOWS_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFQ0050105.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVSDNNVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1459..2514
                     /gene="apbE"
                     /locus_tag="ACHOWS_004633"
     CDS             1459..2514
                     /gene="apbE"
                     /locus_tag="ACHOWS_004633"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFQ0050106.1"
                     /translation="MEISFTRVALLAAALFFVGCDQQPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            2588..3652
                     /gene="ada"
                     /locus_tag="ACHOWS_004634"
     CDS             2588..3652
                     /gene="ada"
                     /locus_tag="ACHOWS_004634"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFQ0050107.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3652..4302
                     /gene="alkB"
                     /locus_tag="ACHOWS_004635"
     CDS             3652..4302
                     /gene="alkB"
                     /locus_tag="ACHOWS_004635"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFQ0050108.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4378..6021
                     /gene="yojI"
                     /locus_tag="ACHOWS_004636"
     CDS             4378..6021
                     /gene="yojI"
                     /locus_tag="ACHOWS_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFQ0050109.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6239..7885
                     /gene="mqo"
                     /locus_tag="ACHOWS_004637"
     CDS             6239..7885
                     /gene="mqo"
                     /locus_tag="ACHOWS_004637"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFQ0050110.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8035..>8481)
                     /gene="eco"
                     /locus_tag="ACHOWS_004638"
     CDS             complement(8035..>8481)
                     /gene="eco"
                     /locus_tag="ACHOWS_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFQ0050111.1"
                     /translation="TTSAWAAESVQPLEKIAPYPQAEKGMKRQVIQLTPQEDESTLKV
                     ELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKF
                     VTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVVR"
BASE COUNT         2030 a         2125 c         2294 g         2032 t
ORIGIN      
        1 tggtcattcc gcaataatct taaaaagttc cctttcattt acattctgaa acatctatag
       61 cgataaatga aacatcttaa aagttttagt atcatattcg tgttggatta ttctgcattt
      121 ttggggagaa tggacttgcc gactgattaa tgagggttaa tcagtatgca gtggcataaa
      181 aaagcaaata aaggcatata acagagggtt aataacatga aagttaaagt actgtccctc
      241 ctggtcccag ctctgctggt agcaggcgca gcaaacgctg ctgaagttta caacaaagac
      301 ggcaacaaat tagatctgta cggtaaagta gacggcctgc actatttctc tgacgacaag
      361 tctgtagatg gcgaccagac ctacatgcgt cttggcttca aaggtgaaac tcaggttact
      421 gaccaactga ccggttacgg ccagtgggaa tatcagatcc agggcaacag cgctgaaaac
      481 gaaaacaact cctggacccg tgtggcattc gcaggtctga aattccagga tgtgggttct
      541 ttcgactacg gtcgtaacta cggcgttgtt tatgacgtaa cttcctggac cgacgtcctg
      601 ccagaattcg gtggcgacac ttacggttct gacaacttca tgcagcagcg tggtaacggc
      661 ttcgcgacct accgtaacac cgacttcttc ggtctggttg acggcctgaa ctttgctgtt
      721 cagtaccagg gtaaaaacgg tagcgtagat ggcgaaggca tgaccaacaa cggtcgtggt
      781 gctctgcgtc agaacggcga cggcgttggc ggttctatca cttatgatta cgaaggcttc
      841 ggtatcggtg ctgcagtgtc cagctccaaa cgtactgacg ctcagaatgg tacctatgta
      901 agcgacaata atgtggtccg taactacatc ggtactggtg accgtgctga aacctacact
      961 ggtggtctga aatacgacgc taacaacatc tacctggctg ctcagtacac ccagacctac
     1021 aacgcaactc gcgtaggttc cctgggttgg gcgaacaaag cacagaactt cgaagctgtt
     1081 gctcagtacc agttcgactt cggtctgcgt ccgtccctgg cttacctgca gtctaaaggt
     1141 aaaaacctgg gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt
     1201 gatgttggtg cgacctacta cttcaacaaa aacatgtcca cctatgttga ctacaaaatc
     1261 aacctgctgg acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta
     1321 gctctgggtc tggtttacca gttctaatct cgattgatat cgaacaaagg gcctgcgggc
     1381 ccttttttca ttgttttcag cgtacaaaca cagttttttg gtgtactctt gcgaccgttc
     1441 gcatgaggat aatcacgtat ggaaataagc tttacccgcg tggcactgct ggctgccgcg
     1501 ctcttctttg ttggttgcga tcaacaacca caacccgcca aaacccacgc tactgaagtt
     1561 accgttcttg aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgac
     1621 gccaaacgca gtgccgaact taaagaaaag attcagaccc aactggacgc tgacgatcag
     1681 ctgctttcga cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg
     1741 tcgccgtggc cggtaagtga agcgatggcc gatatcgtca ctacctcgct gcgcattggc
     1801 gcgaagaccg atggcgcgat ggatataacc gtcgggccgc tggtgaatct gtggggcttt
     1861 ggtccggaac aacaaccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc
     1921 aaaaccggct tacagcacct gacggtcatt aatcagtcgc atcagcaata tctgcaaaaa
     1981 gacctgccgg atttatatgt cgatctctcc accgtcggcg aaggttatgc ggcggatcac
     2041 ctggcacgct tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg
     2101 ctgaacagcc gtggtatgaa cggtgctggc aagccgtggc gggtagcgat tcaaaagcca
     2161 accgataaag aaaacgcggt tcaggccgtg gtggatatca acggtcatgg gattagtacc
     2221 tctggcagct accgtaacta ttacgaactg gacggcaaac gtctttctca tgttatcgat
     2281 ccacaaaccg ggcgtcctat cgaacacaat ctggtatccg tgacggtgat tgccccgacg
     2341 gctctggaag ccgatgcctg ggatactggc ttaatggtac tcggaccgga gaaagccaaa
     2401 gaggttgttc gccgggaagg actggcggtc tacatgatca ccaaagaggg cgataatttt
     2461 aaaacctgga tgtcgccgca gtttaaaagc ttccttatca gcgaaaaaaa ttaaagcgca
     2521 agattgttgg tttttgtgcg atggtgaccg ggcagactaa aggctatcct taaccaggga
     2581 gctgattatg aaatatgcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc
     2641 ccgcgactcg aatgccgacg gcgaattcgt tttcgccgtg cgcaccacgg gtatcttttg
     2701 ccgtccgtct tgccgagcca gacatgcctt gcgggaaaat gtctccttct acgcaaatgc
     2761 cagcgaggcg cttgccgctg gctttcgtcc ctgcaaacgt tgtcagccag acaaagccaa
     2821 tcccctgcaa catcgccttg ataaaattac ccacgcctgc cgacttctgg aacaggaaac
     2881 gcctgtaacg ctggaagcct tagccgacca ggtggcgatg agtccatttc atttgcatcg
     2941 gttgtttaaa gcgactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg
     3001 ccgtttgcgc gaattgctgg cgaaagggga gagcgtgact acatctatta ttaacgccgg
     3061 attccccgac agcagcagtt actaccgcaa agccgacgaa acgctgggca tgacggctaa
     3121 acaattccgt cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct
     3181 gggccgttgc ctggtggcag aaagtgagcg ggggatttgc gcgatattac tgggcgatga
     3241 tgacgcgaca ctaatcagcg agttacagca gatgtttccc gctgccgaca gcgcgcctgc
     3301 tgatcttacg tttcagcaac atgtgcgtga cgtgatcgcc agcctcaatc aacgcgatac
     3361 gccgctgacg ttaccgctgg acattcgcgg cactgctttt cagcaacaag tctggcaggc
     3421 actgcgcacg ataccttgcg gtgaaaccgt cagttatcag caactggcta atgccatcgg
     3481 caaaccgaaa gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt
     3541 aataccctgt catcgggtgg tccgtggtga tggcacactt tccggttacc gctggggcgt
     3601 gtcgcgcaaa gcgcaactgc tgcgccgcga agctgaaaat gaggaaaggt aatgttggat
     3661 ctgtttgccg atgctgaacc gtggcaagaa ccactggcgg ctggagcggt gattttgcac
     3721 cgttttgctt ttaacgctgc ggagcaactg atccgcgata ttaatgacgt tgccagccag
     3781 tcgccgtttc gccagatggt cactccaggc ggatatacca tgtcggtggc gatgaccaac
     3841 tgtgggcatc tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg
     3901 caaacaaata aaccgtggcc cgccatgccg cagagttttc atcatttatg tcaacgtgcg
     3961 gctacggcgg cgggctatcc agatttccag ccagatgcct gtttgatcaa ccgctacgct
     4021 cctggcgcga aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccgatt
     4081 gtttctgttt ctctgggctt acccgcgatt tttcaatttg gcggcctgaa acgaaatgat
     4141 ccgctaaaaa gattgttgct ggaacatggc gatgtggtgg tatggggcgg tgaatcgcgg
     4201 ctgttttatc acggtattca accgttgaaa gcggggtttc atccactcac caccgactgc
     4261 cgctacaacc tgacattccg tcaggcaggt aaaaaagaat aaaaataaga attattattg
     4321 ctgtgcgcgc gaagatgttt aaactgcggg ctgtaattca ttgtccgggt tttctgcatg
     4381 gaacttcttg tacttgtctg gcggcagtat cgctggccat ttatcagtgt gatggcgcta
     4441 agcctcgcca gtgcggcatt aggcattggc ttaattgctt ttatcaatca gcgccttatc
     4501 gaaacggcgg ataccagtct gctggtattg ccggagtttc tggggttatt gctgctgttg
     4561 atggccgtca ctctcggatc gcaactggcg ctcaccactt tggggcatca cttcgtttac
     4621 cgactgcgta gtgaatttat caagcggatt ctcgatactc acgtcgagcg cattgaacaa
     4681 ctcggtagcg cctcgttgct ggcgggatta accagcgatg tgcgcaatat caccattgct
     4741 tttgtgcgtc tgccggaact ggtgcagggg atcattctca ctatcggttc agcggcgtat
     4801 ttgtggatgc tgtcgggcaa aatgttgctg gtaacggcta tctggatggc agtcaccatc
     4861 tggggcggtt ttgtgctggt ggcgcgggtg tacaaacata tggcgaccct gcgtgaaacc
     4921 gaagataagc tgtacacgga ttttcaaact gtactcgaag ggcgcaaaga gctgactctg
     4981 aaccgtgaac gcgccgagta tgtgtttaac aacctctaca ttcctgatgc gcaagagtat
     5041 cgccatcata ttattcgcgc agacaccttc catcttagtg ccgtgaactg gtcaaacatc
     5101 atgatgctgg gcgctatcgg cctggtgttc tggatggcga acagtctcgg ttgggctgat
     5161 accaacgttg ctgcgaccta ttcgttgacg ctgttattcc tgcgtacgcc gctgctttcg
     5221 gcggttggcg cattgccgac gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa
     5281 ttcgcgctcg cgcctttcaa agcagagttt ccgcgcccgc aggcttttcc caactggcaa
     5341 acgctggagc tgcgtaacgt gacgtttagc tatcaggata acgcgttttc cgttgggcca
     5401 attaacctca ccatcaaacg tggcgagctg ctgtttctga ttggtggcaa cggtagtgga
     5461 aaatcgacgc tggcgatgtt gctgacgggc ttgtaccagc cacaaaatgg cgaaatcttg
     5521 ctggatggca aacctgtcag cggcgaacaa ccggaagatt atcgaaaact gttttcggca
     5581 gtgtttaccg atgtctggct gtttgatcaa ctgctggggc cggaggggca acccgctaac
     5641 ccgcaactgg ttgagaagtg gctggcgcag ctgaaaatgg ctcataagct tgagttaagc
     5701 aacgggcgta ttgttaacct gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg
     5761 ctggcgctgg cagaagaacg cgatattatc ctgctggatg aatgggcggc ggatcaggat
     5821 ccacacttcc gtcgtgagtt ttatcaggtg ctgctgccgc tgatgcagga gatgggtaaa
     5881 actattttcg ccatcagtca tgatgatcat tactttatcc acgccgaccg cctgctggaa
     5941 atgcgcaacg ggcaacttag cgagctgaca ggcgaagagc gcgatgctgc ttcgcgtgat
     6001 gccgttgccc ggacggcata atattacgac gctgacctgg cggactgctg ccgccaggtt
     6061 aatatgctaa aaatcccttc atttctaata accctataat taatcaacga aattataatg
     6121 tttctaaaat tagaatataa tttataaaca ttatttaaat gttgttactt aagtgttacc
     6181 gttgatgccg cgcaaatctc acctgcaata aagcgactaa aagtaaggca ttaacaagat
     6241 gaaaaaagtg actgccatgc tcttctcgat ggccgtgggg cttaatgccg tttcgatggc
     6301 ggcaaaagcg aaagcatccg aggagcagga aactgatgta ctgttgattg gcggcggcat
     6361 tatgagcgcc acgttgggga cctatttacg cgagctggag cctgaatggt cgatgaccat
     6421 ggtggagcgc ctggagggtg tcgcgcagga gagttcgaac ggctggaaca acgccggaac
     6481 cgggcattct gcactgatgg aactcaatta cactccgcaa aacgccgatg gcagcatcag
     6541 tattgaaaaa gcagtcgcca ttaacgaagc atttcagatt tcgcgccagt tctgggcgca
     6601 ccaggtcgag cgcggcgtgc tgcgtactcc gcgttcattt atcaataccg ttccgcatat
     6661 gagctttgtc tggggcgagg ataacgtcaa tttcctgcgc gcccgttacg ccgcgttgca
     6721 acaaagctcg ctgtttcgcg gtatgcgtta ctctgaagat cacgcgcaga tcaaagagtg
     6781 ggcaccgtta gtgatggaag ggcgcgatcc gcaacagaaa gtggcagcca cgcgtacgga
     6841 aattggtact gatgtgaact atggcgagat cacccgccag ttaattgctt ccttgcagaa
     6901 gaaatctaat ttttcgctgc aactcagcag cgaagtccgc gccctaaagc gtaatgacga
     6961 taacacctgg accgtcactg ttgccgatct gaaaaatggc actgcacaga acatccgcgc
     7021 caaatttgtc tttatcggcg cgggcggtgc ggcgctgaag ctgttacagg aatcggggat
     7081 tccggaagcg aaagactacg ccggtttccc ggtgggcgga cagttccttg tttcggaaaa
     7141 cccggacgtg gttaatcacc atctggcgaa ggtttacggt aaagcatccg ttggcgcacc
     7201 accgatgtcg gttccgcata tcgatacccg tgttctggac ggtaaacgcg tcgtgctgtt
     7261 tgggccattt gccaccttct caaccaaatt cctcaaaaac ggttcattgt gggatctgat
     7321 gagttccacc accacctcta acgtgatgcc gatgatgcac gtcgggctgg ataatttcga
     7381 tctggtgaaa tatctggtga gtcaggtgat gttgagtgaa gaggatcgtt ttgaagcgtt
     7441 gaaagagtac tatccgcagg cgaaaaaaga ggactggcgt ttgtggcaag cggggcagcg
     7501 cgtgcagatt atcaagcgtg atgccgagaa aggtggcgta ctgcgtctgg gtaccgaagt
     7561 ggtcagcgac caacaaggaa ccattgccgc gctgctgggg gcatcgccag gggcgtcaac
     7621 cgccgcgccg attatgttgg atctgctgga aaaagtattt ggcgatcgcg tttccagccc
     7681 gcaatggcag gctacgttga aagcgatcgt tccgtcttat ggacgcaagc tgaacggtga
     7741 tgtagcggca acagaacgcg agttgcagta caccagcgaa gtgctggggc tgaaatacga
     7801 caaaccgcag gcagcagata gtacgccgaa accgcagttg aaaccgcaac ccgttcaaaa
     7861 agaagtggcg gatattgcgt tgtgatgatg tgccacatcc ggcatggtat gccggatgtg
     7921 gcgtatgcag ataagacgcg tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc
     7981 ggatgcggcg taaccgcctt atccgacctg gcggttgcag cacttcacag cagtttagcg
     8041 aactaccgcg ttgtcaattt tctcttccgc cttccagacg cggtacttca catctacatt
     8101 gtctggcgta tacaccacga tcggcagctt gctgttataa cgcagcattc cggcatcgcc
     8161 cagatacgcg gtgacaaatt tcttctcttt cttgccatcc gggcaggcca tcatcgtcga
     8221 aaccggggaa ctgactttat caaagacata ataatcatag ccccagcctt ccagcgtttt
     8281 gctttccagc ttcccgccga gacgatgcaa attgcaatcg acttccagcg tctggccgat
     8341 taacagttct actttcaggg tagattcatc ttcttgcggg gttaactgaa tcacctggcg
     8401 cttcatgcct ttttcagctt gtggataagg tgcgattttt tccagtggct ggacgctttc
     8461 tgccgcccag gcggaagtgg t
//
LOCUS       DAZKJR010000188         8425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKJR010000188 DAZKJR010000000
VERSION     DAZKJR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.189"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..151
                     /gene="rrf"
                     /locus_tag="ACHOWS_004639"
     rRNA            36..151
                     /gene="rrf"
                     /locus_tag="ACHOWS_004639"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            206..282
                     /locus_tag="ACHOWS_004640"
     tRNA            206..282
                     /locus_tag="ACHOWS_004640"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Asp,seq:gtc)
     gene            444..1247
                     /gene="dkgB"
                     /locus_tag="ACHOWS_004641"
     CDS             444..1247
                     /gene="dkgB"
                     /locus_tag="ACHOWS_004641"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFQ0050112.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(1244..2158)
                     /gene="yafC"
                     /locus_tag="ACHOWS_004642"
     CDS             complement(1244..2158)
                     /gene="yafC"
                     /locus_tag="ACHOWS_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFQ0050113.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            2399..3199
                     /locus_tag="ACHOWS_004643"
     CDS             2399..3199
                     /locus_tag="ACHOWS_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0050114.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3276..4046
                     /locus_tag="ACHOWS_004644"
     CDS             3276..4046
                     /locus_tag="ACHOWS_004644"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0050115.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARHLKNIATRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(4093..5451)
                     /gene="mltD"
                     /locus_tag="ACHOWS_004645"
     CDS             complement(4093..5451)
                     /gene="mltD"
                     /locus_tag="ACHOWS_004645"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFQ0050116.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5523..6278)
                     /gene="gloB"
                     /locus_tag="ACHOWS_004646"
     CDS             complement(5523..6278)
                     /gene="gloB"
                     /locus_tag="ACHOWS_004646"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFQ0050117.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            6312..7034
                     /locus_tag="ACHOWS_004647"
     CDS             6312..7034
                     /locus_tag="ACHOWS_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0050118.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(7031..7498)
                     /gene="rnhA"
                     /locus_tag="ACHOWS_004648"
     CDS             complement(7031..7498)
                     /gene="rnhA"
                     /locus_tag="ACHOWS_004648"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFQ0050119.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            7563..8294
                     /gene="dnaQ"
                     /locus_tag="ACHOWS_004649"
     CDS             7563..8294
                     /gene="dnaQ"
                     /locus_tag="ACHOWS_004649"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFQ0050120.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQDETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
BASE COUNT         2106 a         2061 c         2051 g         2207 t
ORIGIN      
        1 aaccggcgac aagcgggtta ataaaacaga atttgcctgg cggccttagc gcggtggtcc
       61 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
      121 ctccccatgc gagagtaggg aactgccagg catcaaattt agcagtaagc cggtcataaa
      181 accggtggtt gtaaaagaat tcggtggagc ggtagttcag tcggttagaa tacctgcctg
      241 tcacgcaggg ggtcgcgggt tcgagtcccg tccgttccgc cacttattaa gaagcctcga
      301 gttaacgctc gaggtttttt tcgtctgtat ttctattatt gccagaatcg caaaaatcct
      361 ctgcgtttta cgctcttttt ccgcaacagt ctgaagccca taatcacctc agttaacgaa
      421 aatagcatta aaagaggcat attatggcta tccctgcatt tggtttaggt actttccgtc
      481 tgaaagacga cgttgttatt tcatctgtga aaacggcgct tgaacttggt tatcgcgcaa
      541 ttgataccgc acaaatctat gataacgaag ccgcagtagg tcaggcgatt gcagaaagtg
      601 gcgtgccacg tcatgaactc tacatcacca ctaaaatctg gattgaaaat ctcagcaaag
      661 acaaattgat cccgagtctg aaagagagcc tgcaaaaatt gcgtaccgat tatgttgatc
      721 tgacgctaat ccactggccg tcaccaaacg atgaagtctc tgttgaagag tttatgcagg
      781 cgctgctgga agccaaaaaa caagggctga cgcgtgagat cggtatttcc aacttcacga
      841 tcccattgat ggaaaaggcg attgctgctg ttggcgctga aaacatcgct actaaccaga
      901 ttgaactctc tccttatctg caaaaccgta aagtggtcgc ctgggctaaa cagcacggca
      961 tccatattac ttcctatatg acgctggcgt atggtaaggc cctgaaagat gaggttattg
     1021 ctcgtatcgc agctaaacac aatgcgactc cggcacaagt gattctggcg tgggctatgg
     1081 gggaaggtta ctccgtaatt ccttcttcta ctaaacgtaa aaacctggaa agtaatctta
     1141 aggcacaaaa tttacagctt gatgccgaag ataaaaaagc gatcgccgca ctggattgca
     1201 acgaccgcct ggttagcccg gaaggtctgg ctcctgaatg ggattaagcc tctctgacag
     1261 ctcctccggg agctgttttt acatgctcgc taaggaaatc gataaaagcc cggatgcgcg
     1321 tacttaccgc acggtcgctg taatatacgg cactgaatgg catttccact ggcaatactt
     1381 tatctgccat taactccacc aattctccgc gagcgatttc tctgtcgatc atgtagtcgg
     1441 acaaacacgc aatcccgttg ccactcaggc aaagctgttt cagtgtttcc ccactattgg
     1501 atgacaaacc gtacttcacc tcatgtaatt gtccatcact acaggctatc ggccaggtat
     1561 tgagggaagc gggttcagtg aatcccaggc aaacatgttg ctttaaatcg tcgatcgttt
     1621 ctggcttccc gtagcgggaa atataatcgg gggaggcgat aatttttcga taactgttaa
     1681 ataacggcct ggcacgtaag ctggaatccg ttaacgtacc agcgcgtatc gcgacatcca
     1741 cttttctttc gatcaaatta ataatcgttt cggaggagac tagcgataaa gtgacttccg
     1801 gatagcgttc acgaaaaggc ttaatcaacg gcatcagaaa gtgcagcacc actggagttg
     1861 cggcatcgat ccgtaacagt ccacgcggtg tattacgcgt ctccataatt tctgattctg
     1921 ccgctgccat ctcctgcaaa attgactgta cgcgacgaaa ataacgctcg ccttcttccg
     1981 tcaggctaag ttgccgcgtg gtccgattaa gcaggctaac gccaagtttc atctccagct
     2041 ttttcaccgc ccggcttacc gctgagtttg cttgccctaa ttgttccgct gcccggctaa
     2101 agctgccgct ttcgacgact gaaacaaaaa tggcgagttc ttccgacgtg gctttcattt
     2161 ttgctcctgt tgcaaaatag aagagatatt ttgaatttaa ttgtcattaa accatcagga
     2221 tgcgagatat gtcatccgat ttaatgttct caataatgag caaatttctg accggtgtaa
     2281 gcacttgctt acatagcaat atacaattgc tcgttgaaag agtgagctaa aatccctata
     2341 acagtagaac cctcccgagt gcggaagggt tgacgtaata gaggtttcaa agtcaaaagt
     2401 gcgaaaaaac acctatgcca tgcgctatgt tgctggacaa cctgcggaaa ggatcttacc
     2461 gccggggtct tttgcgagca tcggccaggc attaccacct ggagaaccgt taagtaccga
     2521 agagcgtatt cggatcctgg tgtggaacat atacaaacag caacgcgctg aatggttgtc
     2581 ggtattaaag aactacggca aagatgcaca tctggtgtta ttgcaggaag cgcagacaac
     2641 gccagagtta gtgcagtttg cgaccgctaa ctatcttgcc gccgatcagg tacccgcttt
     2701 cgtgctgcca caacatcctt ccggtgtaat gaccctttcg gcggcgcatc cagtgtattg
     2761 ctgcccgtta cgtgaacgag aacccatttt gcgtctggcg aagtcggcac tggtgacggt
     2821 ctatccattg cctgacaccc gcctgttgat ggtggttaat atacacgccg tcaatttcag
     2881 tttgggcgtg gatgtctata gtaagcagtt acttcctatt ggcgatcaga tagctcacca
     2941 cagcggcccg gtcattatgg cgggagattt caatgcctgg agccgtagaa ggatgaacgc
     3001 gttatatcgc tttgcgcggg aaatgtcgct gcgccaggtg cgttttaccg atgatcagcg
     3061 ccgtcgggcg tttggccgcc cgctcgattt tgttttctac cgtggtctga acgtcagcga
     3121 agcttctgta ctggttacgc gcgcttccga tcacaatccg ctactcgttg aattcagtcc
     3181 cggcaagcct gataaataag gtatgtcagg tctgccacag ggcagaccaa cgtttggcgc
     3241 tgcccttttt aatcaaacaa taaaaggaca gcgcaatgac aacacaatcc caccatgacc
     3301 acgtagaaaa gcagtttagc tcgcaggcca gtgagtattt aaccagtacc gtacatgcat
     3361 ccgggcgaga tttacagcgc ctggcggtgc gtctggctga ttatcctgat gcaagtgtac
     3421 ttgatatggg ctgcggagca gggcatgcca gctttgtcgc tgcgcagaac gtgagcgcgg
     3481 tggtggcgta tgacttatct gcccaaatgc tggatgtcgt ggcacaagct gccgaagccc
     3541 ggcacctgaa aaatatcgcc acccgccagg gatatgccga aagtctgcca tttgccgata
     3601 acgcatttga tattgttatc agccgttatt ctgcccatca ctggcatgat gttggtgcag
     3661 cactgcgaga agtgaatagg atattgaaac ctggcggtag gctgattgtg atggacgtaa
     3721 tgtctccggg tcacccagtg cgcgacgtct ggttacagac ggtagaagca ttacgcgata
     3781 cctctcacgt acgaaactac gccagcggtg agtggttgac gttaatcaat gaagccaatc
     3841 tgatagttga taatttaatt acagataagt taccgctgga attttcttca tgggtcgcga
     3901 gaatgcgtac gccagaagcg ttagtagacg ctattcgcat ttaccaacag agcgcatcga
     3961 cagaggtgaa aacgtatttt gccttgcaga atgatggctc tttcaccagt gatatcatca
     4021 tggtagaagc acataaagcg gcataaataa aaaaggcacc gggggaatcg gtgcctttta
     4081 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttatc
     4141 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatt acatctttga tgttcacgcc
     4201 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     4261 gctgttgttt gccagtcgct gtgcgctact gcctgcacca atcgtcaaac tttggcctgg
     4321 cttcagctta gatccgcgca gtttgttcca ctgctgcaaa tctttggtgc ttacgccgag
     4381 acgtgaagcg atacttgaaa gcgtgtcgcc agagcgtacg gtgtaaacac ggctgttaag
     4441 cggcgtattg tcggcaacca gcgtcgactg tacagcagca atttcgcctg aagccagaga
     4501 ttcacgcagt tgatctgcat gcttctttgg caccatcacg tactgcggac cacttgcgcc
     4561 cagcgtggag cctttcacgc ctgcgttgaa tgtcttcagc ttgctgacgg aaatccccgc
     4621 catatctgca accttcgcca tttcaaccgg gctgctcagg tgcacacgtg ccagagcacg
     4681 gctttcatcg gtcgttggca gacgtacgcc ataacgcttg ctgtttttga gaatatcact
     4741 caatgccagc atcttaggca cgtactgctt cgtttcctgc ggcaacggta acgaccagaa
     4801 gtccgtggat ttcccacgcg ctttgttcgt tttaattgcc ttcatgactc gaccttcgcc
     4861 gctgttataa gcagctacgg tcagaagcca gtcgccgtca aacattttgt tcagacgctg
     4921 catcatgttc agcgcggcag ttgttgaagc aacaacatcg cgacgcgcgt cataattgcg
     4981 ggtctgtttc aaaccataat tgcgccccgt gctcggaatg atctgccaga tgcctgcggc
     5041 attggcgcca gacgttgcgt gaggatcaaa agcgctctcc actatgggta gtagtaccag
     5101 ttccataggc atgttacgtt ttttaacttg cccggctatc cagtacatat acggctctgc
     5161 ccgtaaagtt acatcgtgga gatagctctt attgcgtaaa tatttctgtt tctgttcgcg
     5221 aatccggtca ttttccggaa ttcccatctt tagctcgtcg ccaatgaaag cccacaagtc
     5281 accatctggc gcgatagacg tcccatcgtc catccatcgt gcctgacttg taaactttgc
     5341 tgcttcccct tgaccagctg cagaaaggct ctgtgcgtgc tgttgaacgt tgccggtact
     5401 ctggcaaccc acgagcagga cagaggcgag taatatcgct tttgccttca tgtgtgtgtc
     5461 aatagttgct taaaagacga ccgatcataa cggcgaacgg agccgatgac aagaaagttt
     5521 tatcagaacc tatctttctt tgaccttaac catgcaaaac gttcttcagg ttgttgcaat
     5581 aatgtttctt cattaattac attaattaaa tcaatatctt ctgttcttaa aaaaacatta
     5641 atttgccgct catttttcag aattacgggt agtgttattt gattttttgc ccgtaactcc
     5701 ttaactttac gataataatc atttatggac aaatcgtgcg gaagaatact caaagcaaac
     5761 ttcatatttg ataaggtata ttcatgagca caacatacca atgtatcgtc aggtaacgca
     5821 cttaactttt taagtgattg atacatttgt gatgctgtcc cttcaaacaa ccgaccacac
     5881 ccaccagaaa acagcgtgtc gccgcaaaat agataaggtt tactgaagta acagatatgt
     5941 cctaaagtgt gacccggcgt ggcaattaca ctaaattcat gccccaaaac gaaggcagtt
     6001 tcgccatctt tgactacctg tgttgttccc ttatcttgtg tctcttgtgg accatacacc
     6061 acaatttgcg gaaacttttt caccagttct tttacgccgc caacgtgatc gtggtgatgg
     6121 tgggtgagaa atatggcctc cggttgccag ttattggcgg caatggcgtt taatactggc
     6181 tctgcgtctc cgggatcgac aatcaggcag cgacctgctt catcattcaa aacccagatg
     6241 taattgtcat caaaggcggg aatactgtta agattcatag attacctctc agtgtgaaac
     6301 ggaaggttgt gatgaaaccg gcaagagtcc ctcaaactgt cgtggctcct gattgctggg
     6361 gcgatttgcc ctggggagag ctttatcgca aggcgctgga gcgccagctc aacccgtggt
     6421 tcactaaaat gtatggtttt catctgctta agattggcaa tttaagcgca gaaatcaatt
     6481 gcgaagcgtg cgcggtttct catcaagtga atgtttctgc gcaaggactg cccgtccagg
     6541 tgcaggcgga cccacttcat cttccttttg ccgataaatc cgttgatgtt tgtctactgg
     6601 cacatacatt gccgtggtgc accgatccgc atcgtttatt gcgtgaagcc gatcgggtat
     6661 tgattgatga tggctggctg gtgattagtg gcttcaatcc catcagttta atgggattac
     6721 gcaaacttgt gccggtattg cgcaaaacct cgccctataa cagccggatg tttactctga
     6781 tgcggcagct ggactggctc tctttgttga attttgaagt gctacacgcc agccgtttcc
     6841 acgttctccc gtggaacaaa cacggaggaa aactattgaa tgcgcatatt cctgcgcttg
     6901 gttgcttaca acttattgtt gcccggaaac ggaccattcc tttaacgcta aatccgatga
     6961 aacagagtaa aaacaagcca cgaattcgcc aggcggttgg agccacccgg caatgtcgta
     7021 aaccacaggc ttaaacttcg acttgatagc ccgtatcttc cagtgtggga ttcatcgctg
     7081 cggcacgagc cagttcatcg caacgttcgt tttccgggtg tccggcatgg cctttaaccc
     7141 attcccattt gatttgatgc tgccccagcg cagcatcaag acgttgccag agatcgacat
     7201 tttttactgg ttttttgtct gctgttttcc agccacgttt tttccagtta tggatccact
     7261 gggtgatacc ctggcggaca tactggctgt cggtactcaa aatgacttcg caatgttctt
     7321 ttaacgcctc cagcgcgaca atagcggcca tcaactccat acggttgttg gtggtgcggg
     7381 tgtagcctgc gctaaaggtt ttctcgcgtc cgcgatagcg taaaatagcg ccgtaacccc
     7441 caggtccagg attacccaga cacgaaccat cggtgaaaat ttctacctgt ttaagcatct
     7501 ctggtagact tcctgtaatt gaatcgaact gtaaaacgac aagtctgaca taaatgaccg
     7561 ctatgagcac tgcaattaca cgccagatcg ttctcgatac cgaaactacc ggtatgaacc
     7621 agattggtgc gcactatgaa ggccacaaga tcattgagat tggtgccgtt gaagtggtga
     7681 accgtcgcct gacgggcaat aacttccatg tttatctcaa acccgatcgg ctggtggatc
     7741 cggaagcctt tggcgtacat ggtattgccg atgaattttt gctcgataag cccacgtttg
     7801 cagaggtagc cgatgagttc atggactata tccgcggcgc ggagctggtg atccataacg
     7861 cagcgttcga tatcggcttt atggactacg agttttcgtt gcttaagcgc gatattccga
     7921 agaccaatac cttctgtaag gtcaccgata gccttgcggt ggcgaggaaa atgtttcccg
     7981 gtaagcgcaa cagcctcgat gcgttatgtg cccgctacga aatagataac agtaaacgaa
     8041 cgctgcacgg ggcattactc gatgcccaga tccttgcgga agtttatctg gcgatgactg
     8101 gtgggcaaac gtcgatggct tttgcgatgg aaggagagac acaacagcaa caagatgaaa
     8161 caacaattca gcgcattgtg cgtcaggcaa gtaagttacg cgttgttttt gcgacagatg
     8221 aagagcttgc agcacatgaa gcccgtctcg atctggtgca gaagaagggg ggaagctgcc
     8281 tctggcgggc ataaatacct gtaaaaggcg ctaaaaatag cgacttgggc gatttttgca
     8341 gcaaacgatt caaaagatga gaaaaaccgt tgacgaaggg tgaggcaatc cgtaatattc
     8401 gcctcgttcc caacggaaca aaacg
//
LOCUS       DAZKJR010000189         8420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKJR010000189 DAZKJR010000000
VERSION     DAZKJR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.190"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            110..2488
                     /gene="ppsA"
                     /locus_tag="ACHOWS_004650"
     CDS             110..2488
                     /gene="ppsA"
                     /locus_tag="ACHOWS_004650"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFQ0050121.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2545..4191)
                     /gene="fadK"
                     /locus_tag="ACHOWS_004651"
     CDS             complement(2545..4191)
                     /gene="fadK"
                     /locus_tag="ACHOWS_004651"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFQ0050122.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFIHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4248..4541)
                     /locus_tag="ACHOWS_004652"
     CDS             complement(4248..4541)
                     /locus_tag="ACHOWS_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFQ0050123.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(4538..5827)
                     /locus_tag="ACHOWS_004653"
     CDS             complement(4538..5827)
                     /locus_tag="ACHOWS_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0050124.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLDAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5882..6820)
                     /locus_tag="ACHOWS_004654"
     CDS             complement(5882..6820)
                     /locus_tag="ACHOWS_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFQ0050125.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSN
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6840..7604)
                     /locus_tag="ACHOWS_004655"
     CDS             complement(6840..7604)
                     /locus_tag="ACHOWS_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFQ0050126.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7920..>8420
                     /locus_tag="ACHOWS_004656"
     CDS             7920..>8420
                     /locus_tag="ACHOWS_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFQ0050127.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         2001 a         2179 c         2186 g         2054 t
ORIGIN      
        1 tatatttgct tgaacgattc accgtttttt tcatccggtt aaatatgcaa agataaatgc
       61 gcagaaatgt gtttctcaaa ccgttcattt atcacaaaag gattgttcga tgtccaacaa
      121 tggctcgtca ccgctggtgc tttggtataa ccaactcggc atgaatgatg tagacagggt
      181 tgggggcaaa aatgcctccc tgggtgaaat gattactaac ctttccggaa tgggtgtttc
      241 cgttccgaat ggtttcgcca caaccgccga cgcgtttaac cagtttctgg accaaagcgg
      301 cgtaaaccag cgcatttatg aactgctgga taaaacggat attgacgatg ttacccagct
      361 tgcgaaagcg ggcgcgcaaa tccgccagtg gattatcgac actcccttcc agcctgagct
      421 ggaaaacgcc atccgcgaag cctatgcaca gctttccgcc gatgacgaaa acgcctcgtt
      481 tgcggtgcgt tcctccgcta ctgcagaaga tatgccggac gcttcttttg ccggtcagca
      541 ggaaactttc ctcaacgttc agggttttga cgccgttctc gtggctgtga aacatgtatt
      601 tgcttctctg tttaacgatc gcgccatctc ttatcgtgtg caccagggtt acgatcaccg
      661 tggcgtggcg ctgtccgccg gtgttcagcg gatggtgcgc tccgacctcg catcttctgg
      721 cgtgatgttc tccattgata ccgaatccgg ctttgaccag gtggtgttta tcacttccgc
      781 atggggcctt ggtgaaatgg tcgtgcaggg tgcggttaac ccggatgagt tttacgtgca
      841 caaaccgaca ctggcggcga atcgcccggc tatcgtgcgc cgcaccatgg ggtcgaaaaa
      901 aatccgcatg gtttacgcgc cgacccagga gcacggcaag caggttaaaa tcgaggacgt
      961 accgcaagaa cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa
     1021 acaggccgta caaattgaga aacactacgg tcgcccaatg gatattgagt gggcgaaaga
     1081 tggtcacacc ggtaaactgt tcattgtgca ggcgcgtccg gaaaccgtgc gctcacgcgg
     1141 tcaggtcatg gagcgttata cgctgcattc acagggtaag attatcgccg aaggccgtgc
     1201 tatcggtcat cgcatcggtg cgggtccggt gaaagtcatc catgacatca gcgaaatgaa
     1261 ccgcatcgaa cctggagacg tgctggtcac tgacatgacc gacccggact gggaaccgat
     1321 catgaagaaa gcatctgcca tcgtcaccaa ccgtggcggt cgtacctgtc acgcggcgat
     1381 catcgctcgt gaactcggca ttccggcggt agtgggctgt ggtgatgcaa cagaacggat
     1441 gaaagacggt gagaacgtca ctgtttcttg tgccgaaggt gataccggtt acgtctatgc
     1501 ggagttgctg gaatttagcg tgaaaagctc cagcgtagaa acgatgcctg atctgccgtt
     1561 gaaggtgatg atgaacgtcg gtaacccgga ccgtgctttc gacttcgcct gcctgccgaa
     1621 cgaaggcgtc ggccttgcgc gtctggaatt tatcatcaac cgtatgattg gcgtccaccc
     1681 acgcgcactg cttgagtttg acgatcagga accgcagttg caaaacgaaa tccgcgagat
     1741 gatgaaaggt tttgattctc cgcgtgaatt ttacgttggg cgtctgactg aagggatcgc
     1801 gacgctgggt gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg attttaaatc
     1861 gaacgaatat gccaacctgg tcggtggtga gcgttacgag ccagatgaag agaacccgat
     1921 gctcggcttc cgtggcgcgg gccgctatgt ttccgacagc ttccgcgact gcttcgcgct
     1981 ggagtgtgaa gcagtgaaac gtgtgcgcaa cgacatgggg ctgaccaacg ttgagatcat
     2041 gatcccgttc gtgcgtaccg tagatcaggc gaaagcggtg gttgaggaac tggcgcgtca
     2101 ggggctgaaa cgtggtgaga acgggctgaa aatcatcatg atgtgtgaaa ttccgtccaa
     2161 cgccttgctg gcagagcagt tcctcgaata tttcgacggc ttctcaattg gctcaaacga
     2221 catgacacag ctggcgctcg gtctggaccg tgactccgga gtggtgtctg aactgttcga
     2281 tgagcgcaac gatgcggtga aagcactgct gtcgatggcg attcgtgccg cgaagaaaca
     2341 gggcaaatat gtcgggattt gcggtcaggg tccgtccgac cacgaagact ttgccgcatg
     2401 gttgatggaa gaggggatcg atagcctgtc tctgaacccg gataccgtgg tgcaaacctg
     2461 gttaagcctg gctgaactga agaaataaaa taaatccccg gcggcgtttt gtcgccgggt
     2521 ttatgtgatc cccgaagatg aaacttattc aatctcttca cagacatcct gagttaatcg
     2581 ccgcataata tctttcctta acaaaaactt ttgtatttta cctgaggctg tgcgcggtag
     2641 tttttcgatt accacgatat gttcaggata tttatatttc gcgacccgtt tacggctaaa
     2701 aaatgccact acatcttcca gcgataatga atgatgcggt gctttcagca cgacataagc
     2761 gcatgaacgt tcacctaagc gttcatcggg cattgcaacc acacaagcat cgtgaatttt
     2821 aggatgctgc aataaaatat cttctacttc acggctgcta atattttcgc cgccgcggac
     2881 aataatatct ttcttgcgcc cggttatttt tatatagcca gcctcatcca tgcggcagag
     2941 atcgccgctg taataccagc cttcttcatc cagcgcacgg gcggttaatt caggttcatc
     3001 aaaatacccc ataaacacat tggggccacg cgaggcttct tcaccttcgc aacctggcgg
     3061 taaagtcttg cgtgcgtcat cgaccacttt aatctctaca cctgcggcag cgtaaccatc
     3121 ggtgtgtata aagcgcgaca aaggatcatc gagattcacc accgcatgcg gcgaactttc
     3181 tgtggaacca taaacactta ataatttaat gccgcgctgc tggcattcac gcgccacttt
     3241 tttggggatt gtggtaccgc cgcaaagaaa gaaacgcagc gctgaaaggt ctgcgggctg
     3301 tttctccagt aaattcaaaa gatcatagac aaacggcgtt gcgccgagca tacaggtgca
     3361 acgctgctgc tcaagcagcg cgagacacgc agcaggagtg aaaatatcta acaacacgct
     3421 gcgagcacca atcaaaaaag gtgctgttac gccatgcaga aagcccgttg cgtgaccaag
     3481 tggcgcaggc atcataaaga catcctgcca ggtcagattt agccgcgcgc aataagcccg
     3541 ctcactggcg agaatattgt tatgcgttag catcacgccc tttggcagac cctcggttcc
     3601 ggaggtaaac agcaccgcgg ctaattcatc gccgtggacc gttatcgccg tggtcagagg
     3661 agtattgtcg gcgataatct gacttaatga gagagcagag gtggcgggag ccagtttgtc
     3721 cacgccgaca atttgttgta gttgtggaag ctgattttgc agcggcagga ttaaatctac
     3781 cggacgcgtt tgtttaaaca acgtcggtgc aaagaacatt tttgcctgac acttattgag
     3841 cacccacacc agttctgctt ctcgccagga aggcaacagc ggcacggaaa ccgcaccggt
     3901 tttcaggcag gcaagataga taacggtaaa ttcacaccag cctggcagtt gaaatgcgat
     3961 gcgatcgcct gattcaatac ccttcgctaa catccagttt gccagacagc tcgcggcgtg
     4021 atcgagcgcg ctataagtgt acgttgcacc atgattgtcg accacggcaa ttttgtctgg
     4081 catcgcacga gcggtctgct gccagtaatc ggccagtgaa gcatcgcccc ataacccttg
     4141 ctgacgatac gccgcacgac gttgttcgtt gaacgttaat gtcactttca ttttggactc
     4201 tcgaaacaga acatcaggcc cgagatgcgg gcctgtggga tgcatgatta gccgtagcga
     4261 aattcaatac cgaaggtgcc tgcgggatat tgccactgtt cgagaatagt gttgccgcac
     4321 agcacccgac aggtgccgca ctccagacaa ccggcggaat cgaaatgaat gtttcctgcg
     4381 tcatcctgct tataaagtcc ggcagggcag gctttcatta atttacggaa ttcattgata
     4441 tcgggatttg ccgccagaat gatatgcggg tggccctcat caacatggaa tttatttacg
     4501 cctaatttga tgtcgacgtt aaccgtagcg ttctggctca tagcgcggtt gctcccttaa
     4561 tgccatcttt cagcaagttg atcagtccaa ttttcttcgc gtgtcccatg atcattttgc
     4621 gtaccggttg gttaggtttg ccgtcgatgg tgaacatctc gttcatgatg tcggcgacca
     4681 ttcgtgggta ttggctaaac aggcgcgggt tttccatcag cgccgggatc ttgcgaaaat
     4741 gctgcatatc acgcatgacg cagctttgtt ccagctcacg tttgtattgc gcaagactgc
     4801 tggcggagaa atccgcgcgt tctttggcgg cgatcactgt tgtggcggca gcctgagccg
     4861 atgcaatggc taaatccatg ccgcggaccg taaaacccaa attcaggcag aagcctgcgg
     4921 cgtcaccaac gatcatcacg ccatcgttaa ccagctgcgg caccattgcc agaccgccct
     4981 ccggcaccat atgcgcggag tattcaagca gtttgccgcc gctaatcagc gggcgaatgg
     5041 cggggtgttg tttaaaatct tccagcattt gcggcacact tttttgcgca tgggcgatat
     5101 cacccaatcc acaaaccagc cccaacgata cggaatcttt gttggtatag aggaatccgc
     5161 cgcccatcag gccgtcagaa ggggaaccgg caaacagcca ggcggcacct tcctcgcccg
     5221 taacattaaa gcgatcgttg atctgttctg gtgtcaggcc tataacctct ttaacaccaa
     5281 cagcgtaatg atgcggatcg gaagcggaaa ccattcccag cgagcggcca agcatcgagt
     5341 taacgccatc agccagaatc accacattcg cttcgagaat atcatccccg gcctggacgc
     5401 cagtgacctt gtttccttca cgaaccagtg catcgacgcg tacgcccggg ataaactgcg
     5461 cgcccgcctg ctcggcttgt tccatcaacc acgggtccag acgattacgc aatacggtat
     5521 aagacgcgtg ttgcggaaca tctggttgct cgcggtgaaa atcgagggta acggcgcttt
     5581 cttcggttaa gaaggagatt ttctcgcgtg tgaccttgcg ttctaccggc gctgatgctg
     5641 caaagcctgg aataattgcg tcaagtgtgt gggcataaag acgcccgccg gtcatgtttt
     5701 tacatccggc actgtcgccg cgttctatca ccaggacatc cagcccggct cgcgccatga
     5761 ccagtgcggc aacgctaccg gcaacgcccg caccgaccac aatggcatca aatttgtcat
     5821 ccgacatgcg ttatgctccc ttcccgctgc gtttacagcg ggagatgtgg ttgaaaggga
     5881 atcagcgact caactggcta atcagggcag ggacgacttt gtagatatcg cccaccagac
     5941 cgtagtcggc atagttgaag attggcgcat ttttatcttt attaatggcg acaatcactt
     6001 ttgcgccgtt gccgccaacc atatgctgga tctgcccgga gatccccagc gtcaggtaga
     6061 gatcggattt cagcaacacg ccggagacac cgatataacg ttcacgctcc atccagttct
     6121 cgccttcggc aattggacgt gaacagccga cttcagcatt cagcaccgcc gccagttcgt
     6181 ggaccatttt caggtcatcc tgcgctgcca gaccacgacc gacgccaacc acacgttttg
     6241 ctttgctcag gtccacgctg cttgccgctt tcgcacggcg ttcgcgacag agaatttcat
     6301 gacgcggggc aacataaggt accgtttctg tcggacactg attagaggta tcactggtgc
     6361 acggttcctg aacaccgggc gcaagggtaa tgatcgccag cgggctattg atcttttcct
     6421 gtgcgaacgc taacccgcca tacatccggt gttcggcgca aatgtgacca tcgacaatat
     6481 ccaccgcagt ggcatcgttc accagcgccg cattcagttg cacacttaac cgtgcagcca
     6541 gagctttacc gcgtttcgtc gcggccagca gcaacataga agggtgttta tctttcagca
     6601 gggcagcaat gctttcggcg taattttcag tacgttgcag cgcgtcgttt tgcgcaagaa
     6661 cataaatgca ttttggccca taaggcataa ctgtctgcgc ctggtcggta ttttgtacaa
     6721 tggcatacac ctgttggccc cattgctgag cgccgccaaa cagttcagca taacgttcag
     6781 gattatcgct aaagacccag acgctgttta attgactcat cgtttcttct ccgtaggctt
     6841 cagttcaggg ctttcttcag atgttccgcc agctcggcaa tggcctccgg cgaatcgtta
     6901 tcgagaatga tgtgcttacg ttctgtttgc ggcggtacgc gaatgccggt cagttcagca
     6961 agtggtgcgc tctggctcca gccaatatca cttgcctgcc actgatttac cggtttttta
     7021 cccgcgccga ggatggcttt catcgaagga atacgtggta cgttaatatc ggaggtgacg
     7081 cagagcacgg ctggaacaga gagttcaata acttcaacat catcttcaag cgtgcgttca
     7141 atcaccagtg tattgccctg acgctgaatg gcactcactg cattaatcac cggaagttgc
     7201 agaatttctc cgaccagcaa gccaacctgc tgggcataaa ggtcgccgga accttcacca
     7261 aagatcagta aatcgaagcc gatcttttca actgctgtcg ccagcgcctt tgcggtatcg
     7321 agaggcagtg catgctcaag ttgtgcatcc tgcaccatat acaggctgtg cggcccgcgg
     7381 gatagcacgt ctttgcgcac tttcgagttc tgcaacaaag agccgccgac ggtcagcgcg
     7441 gctatctcat cgtcatctgt tgcaagctgg cttgcagctt caatggcatt gagatcgaac
     7501 tggctgattt tggcgtcggc attgtcgaaa ttcagggtat attctggagt gacaacaatg
     7561 tcctgttctt caggcaccag cttaaagcag gttattattt tcatgatttc tcctgtgagt
     7621 cgcatacgga accatctccg atggaaggta aaaatggcct gaaaatagtg tgcgatagcg
     7681 gtgcggggtt ttcgacggag caagctgttt tccttgccaa caatcttgca aaaaaaatgc
     7741 gatcgctaaa acaaaagtgc aatttgcggc aatgttgtta ttccatgacc gtacaatctt
     7801 gcattcagtg taaataatct tgtttagctc tcgctaaacg cggttgtata accgcaatac
     7861 acgccgtatt ttatccggca tatattgttt ttgcttgctc atggaatagg gaggttgtca
     7921 tgtatcaacg ctgttttgat aacgccagcg aaacgctgtt tgtcgccggt aaaacgccac
     7981 ggctttcacg ttttgcattt agcgatgatc caaaatggga gtccggacat cacgttcatg
     8041 acaatgaaac cgagttgatt tacgtcaaga aaggggtagc aaggtttacc atcgattctt
     8101 cgttatatgt cgcgcatgcc gatgacattg tggtgataga acgtggcagg ttgcatgcgg
     8161 tggcctctga cgttaacgat ccagcaacga cgtgtacctg tgcgctgtac ggctttcagt
     8221 ttcagggggc tgaggaaaat cagctactgc aaccgcattc ttgcccggta attgccgcag
     8281 ggcagggaaa agaagtcatt aaaaccttat ttaatgagct aagtgtgatt ttgccgcaaa
     8341 gtaaaaatag ccaaacatct tcgttatggg acgcatttgc ctatacgtta gcaattcttt
     8401 actatgaaaa ctttaaaaat
//
LOCUS       DAZKJR010000190         8403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKJR010000190 DAZKJR010000000
VERSION     DAZKJR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.191"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..789
                     /locus_tag="ACHOWS_004657"
     CDS             <1..789
                     /locus_tag="ACHOWS_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFQ0050128.1"
                     /translation="LEGDLLRTEVAGMSVTAGIYGAAGHSSVDVKDDDASRAGTVRDD
                     AGSLGGYLNLTHTSSGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETALPFSI
                     TDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGYGSAQHVRAGFRLGSHNDMTFGEGTS
                     SRDTLRDSTKHGVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSL
                     DLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            910..3426
                     /locus_tag="ACHOWS_004658"
     CDS             910..3426
                     /locus_tag="ACHOWS_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050129.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLNGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDATLQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLVLVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVAGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRQEEALLPELPA"
     gene            3502..3963
                     /locus_tag="ACHOWS_004659"
     CDS             3502..3963
                     /locus_tag="ACHOWS_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFQ0050130.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKMLTDP"
     gene            4099..4188
                     /locus_tag="ACHOWS_004660"
     CDS             4099..4188
                     /locus_tag="ACHOWS_004660"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFQ0050131.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            4366..5187
                     /locus_tag="ACHOWS_004661"
     CDS             4366..5187
                     /locus_tag="ACHOWS_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFQ0050132.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            5187..5387
                     /locus_tag="ACHOWS_004662"
     CDS             5187..5387
                     /locus_tag="ACHOWS_004662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050133.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            5521..5994
                     /locus_tag="ACHOWS_004663"
     CDS             5521..5994
                     /locus_tag="ACHOWS_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFQ0050134.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            6009..6485
                     /gene="radC"
                     /locus_tag="ACHOWS_004664"
     CDS             6009..6485
                     /gene="radC"
                     /locus_tag="ACHOWS_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0050135.1"
                     /translation="MQQLSFLPGAMTPGELSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            6554..6775
                     /gene="yeeT"
                     /locus_tag="ACHOWS_004665"
     CDS             6554..6775
                     /gene="yeeT"
                     /locus_tag="ACHOWS_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFQ0050136.1"
                     /translation="MKIVTRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            6851..7219
                     /gene="cbeA"
                     /locus_tag="ACHOWS_004666"
     CDS             6851..7219
                     /gene="cbeA"
                     /locus_tag="ACHOWS_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFQ0050137.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            7308..7685
                     /gene="cbtA"
                     /locus_tag="ACHOWS_004667"
     CDS             7308..7685
                     /gene="cbtA"
                     /locus_tag="ACHOWS_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFQ0050138.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRVTG
                     LMTRDNYRTVNNITLGKYQEAKR"
     gene            7682..8170
                     /locus_tag="ACHOWS_004668"
     CDS             7682..8170
                     /locus_tag="ACHOWS_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFQ0050139.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LRVVEESDQQSTCTPPPFATLAGIRCSTAHITETDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAGCLPGLPTF
                     DW"
     gene            8182..8379
                     /locus_tag="ACHOWS_004669"
     CDS             8182..8379
                     /locus_tag="ACHOWS_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFQ0050140.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
BASE COUNT         1960 a         2278 c         2241 g         1924 t
ORIGIN      
        1 ctggagggtg acctgctgag aacagaggtt gccggtatgt ctgtgaccgc agggatatat
       61 ggtgctgcag gccattcttc cgttgatgtt aaggatgatg acgcctcccg tgccggcacg
      121 gtccgggatg atgccggcag ccttggcgga tacctgaatc tgacacatac atcctccggc
      181 ctgtgggctg acattgtggc cctgggaacc cgccacagca tgaaagcgtc aacggacaat
      241 aacgacttcc gcgcccgggg gtggggctgg ctgggttcac tggaaaccgc tctgcccttc
      301 agtatcactg acaatctgat gctggagccg caactgcagt acacctggca gggactctcc
      361 ctggatgacg gccaggataa cgccggttat gtgaagttcg ggtatggcag tgcacaacat
      421 gtgcgtgccg gtttccgtct gggcagccac aacgatatga cctttggtga aggcacctca
      481 tcccgtgaca ccctgcgcga cagtacaaaa cacggtgtga gtgaactgcc tgtgaactgg
      541 tgggtacaac cttctgttat ccgcaccttc agctcccggg gggacatgag catgggtaca
      601 gctgcagccg gcagtaacat gacgttctca ccgtcccgga atggcacgtc actggacctg
      661 caggccggac tggaagcccg tgtccgggaa aatatcaccc tgggcgttca ggccggttat
      721 gcccacagcg tcagcggcag cagcgctgaa gggtataacg gtcaggccac actgaatgtg
      781 accttctgac agaaccatcg cctctctgtg gtcccggtca tcatgaccgg gacccggacc
      841 ggcgcaacgg atcttcaacg ccacattcgc tggcattaac aataacatga tattcatcac
      901 ggagtgacta tgttacagat agtcggcgcg ctgatcctgc tgatcgcagg atttgccatt
      961 cttcgccttt tgttcagagc attaatcagc acggcttctg cgctggcagg gctcatattg
     1021 ctgtgtctgt tcggcccggc cttactggct ggctatatca ccgaacgcat aacccggttg
     1081 ttccatattc gctggctggc aggcgtattt ctgacgattg ccggaatgat catcagcttc
     1141 atgtggggac ttgatggtaa acatatcgcg ctggaggctc acacctttga ctctgtgaaa
     1201 tttattctga ccaccgctct cgccggtggt ctgctggctg ttcccctgca gatcaaaaac
     1261 attcagcaga acgggatcac accagaagat atcagcaagg aaattaacgg gtattactgc
     1321 tgtttttata ctgccttttt ccttatggcg tgttctgcat gcgcaccatt gatcgcgtta
     1381 cagtacgata tttcaccgtc actgatgtgg tggggcgggt tgttgtactg gctggctgca
     1441 ttagtgacgc tgctatgggc ggccagccag atccaggcgc tgaaaaaact gacctgtgcc
     1501 atcagccaga cactggaaga acaaccggtg ctcaacagta aatcgtggct gaccagtttg
     1561 caaaacgatt acagccttcc tgactcactg acggagcgca tctggctgac gctcatttct
     1621 caacggattt cccggggaga gctgagggaa tttgaactgg cagacggaaa ctggttactg
     1681 aacaatgcct ggtatgaaag aaacatggca gggtttaacg aacagttgaa agagaacctg
     1741 tcattcacac ctgatgaact gaaaacgctc ttccggaacc gcctgaattt atcaccggaa
     1801 gcgaatgacg attttctcga tcgttgcctg aacggcggtg actggtatcc cttttcagaa
     1861 ggtcgccgtt ttgtatcatt ccatcacgtg gatgagcttc gtatctgtgc ctcctgcggg
     1921 ctgacagaag tacatcatgc cccggaaaat cataagccgg atccggaatg gtactgctcc
     1981 tctctttgtc gcgaaacaga aacactgtgt caggaaattt atgaacgccc ttacaacagc
     2041 tttatttccg atgcaacggc gaatggtctg attctcatga aactgccgga aacctggagt
     2101 acaaatgaga aaatgtttgc ttccggaggg cagggacatg ggtttgccgc tgaacgggga
     2161 aaccatattg tcgacagagt ccgtctgaaa aacgcacgga tcctcggtga taataatgcc
     2221 agaaatggag cagacagact ggtcagcgga acagaaatcc agacgaaata ttgttcaact
     2281 gcagcccgta gcgtcggtgc ggcattcgac ggacaaaacg gacagtatcg ttacatggga
     2341 aataatggcc ccatgcaact ggaagtcccc cgtgatcagt atgccggcgc tgttgaaacc
     2401 atgaggaata agatccgcga aggtaaagta cccggtgtaa ccgatcccgc agaagcgtcc
     2461 cggctgattc gtcggggaca tctgacttat actcaggccc gtaatatcac ccggttcggg
     2521 accatcgaat cggtcactta tgatattgcc gagggttcgg ttgtcagtct ggcggccgga
     2581 gggatcagtt ttgccctgac ggcatcggtc ttctggctca gcaccggcga tcgcgatgct
     2641 accctgcaga cagctgctgt ccaggcagga aaaaccttca cccgcacact ggctgtctac
     2701 gtcacaaccc agcaactcca ccggctcagt gttgttcagg gtatgctgaa gcatattgat
     2761 ttttcgacgg ccagcccgac tgtccggcag gcgcttcaga aggggaccgg tgcaggaaat
     2821 atcagtgccc tgaacaaagt gatgaagggt acgctggtga catctctggt actggtcgct
     2881 gtcacaaccg gccctgacat gatcaaaatg ttgcggggac ggatctccgg tgcgcagttc
     2941 atcaggaatc ttgccgtggc atcttccggt gtggcaggtg gtgctgtcgg gtcagtggcg
     3001 ggcgggatac tgttcagtcc actgggacca tttggtgcac tgacagggcg tgtggctggc
     3061 ggtgttctgg ggggaatgat tgcctccgct gtatcaggaa aaattgccgg agcgctggtt
     3121 gaagaagatc gcgtcaaaat tctggcaatg attcaggagc aggtgacatg gcttgccggc
     3181 agtttcctgc tgaccggaca tgagattgaa aatctgaacg cgaatctggc ccgtgttatc
     3241 gatcagaatg ctctggagat cattttcgcc gccggtatac aacaacgggc cgcgaccaat
     3301 atgttaatca aaccactggt ggtcagtatc atcaggcaac gccccgtcat ggaatatgat
     3361 gcatcccatc tcggcaagat ggttaaccga caggaagaag cattactccc ggagttaccg
     3421 gcataacggc tattctgcgc cttttacgtt ttatcacctg atttgtcagt gtcatcgtca
     3481 tgactcagag aggtaaatac catgattcac ctgttcaaaa cctgcatgat taccgccttc
     3541 attctggggt taacgtggtc tgccccactc cgggcacagg atcaacgtta catcagtata
     3601 cgcaatacag atacgatatg gctcccggga aatatttgtg cttaccagtt ccggctggat
     3661 aatggcggaa acgatgaagg atttggcccc ctcaccatca ctctgcaact caaagacaaa
     3721 tatggtcaga cgctggtgac cagaaaaatg gaaacggaag cctttggtga cagtaatgcc
     3781 acgcgaacca cagacgcatt tctggaaacg gagtgcgtgg aaaatgtcgc cacaaccgaa
     3841 atcattaaag caactgaaga aagtaacggc catcgtgtca gtctgccgtt atcggttttc
     3901 gatccccagg actaccatcc actgctgatt accgtttccg gaaaaatgtt aactgatccc
     3961 tgaagtcagt ttcatgcttc agcctctgcc acaacgtatg gcagaggcta tttatcagga
     4021 gaaaccagat atgccgggtt acacacagag tatgtcctgg ctgaaggatc tttctcatac
     4081 gggcaggcgg ttgctgtcat aaccgcttac cgcaatgtgt ttatccagga tgacccggga
     4141 atgcatttcc gtcgggttat ccgcaacgcg gaaggtcagc gacgctgacg ctgccggaac
     4201 tctgaagctg atgccggaaa acagcttaat gcgtggctcg ccagtggagg gcttctcaga
     4261 caataaacgt cattgatttc gtccatcagg ccgcgtcttc tccgggagac gcggcttttt
     4321 catttattgc accagtaaat cttaaccacc gataaggagc aaagtatgcg attagtcagt
     4381 cgttttggtt atgctgcaaa ccagatacgc cgtgaccgtc cgctgacaca tgaagagctg
     4441 atgcaccatg tgccggggat tttcggggaa gagaaacaca catcccgaag ccagaactat
     4501 acgtacatcc ccaccatcac cgtactggaa agtctgcagc gggaaggctt ccagcccttt
     4561 ttcgcctgcc agacccgtgt gcgcgacccg ggccgccggg gatacacaaa acacatgctg
     4621 cgtctgcggc gggccggaga gataaacgga gaacatgtcc ctgaaattat tctgctcaac
     4681 tcccatgacg gtacctccag ctaccagatg ctgccgggtt acttccggtt cgtctgccag
     4741 aacgggtgcg tctgtggtca gtctctgggg gaagtgcgtg ttccacaccg gggaaatgtg
     4801 gtgcagaaag tcattgaagg ggcttacgag gtggtgggcg tttttgaccg gattgaagag
     4861 aaacgtgatg ccatgcagtc gctggtcctg ccgccaccgg cacgccaggc gctggcacag
     4921 gcggcactga cttaccgtta tggtgacgaa catcagcccg tcaccaccgc cgacattctg
     4981 acgccacgac gtcgggagga ttacggtaaa gacctgtgga gcgcatatca gaccatccag
     5041 gagaatatgc tgaaaggcgg gatttccggt cgcagtgcaa aaggaaaacg tatccacacc
     5101 cgtgccattc acagcatcga caccgatatt aagctcaacc gcgcattgtg ggtgatggca
     5161 gaaacgctgc tggagagcat gcgctgatga aacatatccc tgaaggcgtg attcaggcac
     5221 ccgtatcacc cgacgccccg gaggcgctgc cgggatgtcg ttccatttct gatgatgacc
     5281 tgtgcgcctg gtgtacccgt ctgctttacc gtcccggaga actcagtctg tgcagactga
     5341 gtacaggcaa tggcatctgg ccatccgtct gtgaccggaa cggttaacgg ctgtccggaa
     5401 tttcagccga atatcattca tccctgatat tgccgggtct cacggtcagt gatgaacgtg
     5461 atgttgtcag cccccggcct ttatttcctc tcatttattc acattacaag gatttcacac
     5521 atgaaaacat tgtctcagaa caccacctct tcagcctgtg caccggagac tggcctgcaa
     5581 caactggttg ccaccatagt ccctgatgaa cagcgcatca gcttctggcc gcagcatttt
     5641 ggcctcattc cacagtgggt gaccctggag ccccgtgttt tcggctggat ggaccgtctt
     5701 tgcgaagact actgtggtgg tatctggaat ctgtataccc tgaacaacgg cggggcattt
     5761 atggcacccg aaccggatga cgatgatgac gaaacatggg tactgttcaa tgccatgaac
     5821 ggtaaccgcg ctgaaatgag cccggaagcc gccggcattg ccgcctgtct gatgacgtac
     5881 agtcatcacg cctgtcgtac ggagtgttat gccatgacgg tccattatta ccggctgcgg
     5941 gattacgccc tgcagcatcc ggaatgcagc gccattatgc gcatcatcga ctgagcagga
     6001 gaagtacaat gcaacaactt tcctttctgc ccggcgcgat gacgcccggc gagctcagcc
     6061 tcattctgcg ggccctgaaa accctggacc gccatcttca tgaacccggc gtggccttca
     6121 cctccacccg agcggcacgg gaatggctga ttctgaacat ggcgggactg gagcgtgaag
     6181 aattccgggt gctgtatctg aacaaccaga atcagctgat tgccggtgaa accctcttta
     6241 ccggcaccat caaccgcacg gaagtccatc cccgggaagt gattaaacgc gccctgtacc
     6301 acaatgccgc tgccgtggtg ctggcacaca atcacccgtc cggtgaagtc acacccagta
     6361 aggcagaccg gcttatcacc gaacgtctgg tacaggcgct ggccctggtt gacatccggg
     6421 tgccggacca tctgatagtc ggtggcagcc aggttttctc ctttgcggaa cacggtctgc
     6481 tttaacctgt caccgtcaca accacatcat acctgttttc gcttttatct tctgtcttca
     6541 gaggtatcac actataaaaa tcgtcacccg tggtgaagcc atgcgtattc accaacaaca
     6601 tccgacatcc cgtctttttc cgttctgcac cggcaagtac cgctggcacg gcagtgccgg
     6661 ggcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg
     6721 ccataaggac agttttggcc cgtatgtccg gctgatgagc gtcaccctga actgaatcag
     6781 ggcattctga tgagcagaat tatcgccacc accggaccat tcttaaccga ttttttgtga
     6841 ggactttatc gtgtcagaca cactccccgg gacaacgcat cccgacgaca atcacgaccg
     6901 cccctggtgg gggctgccct gcaccgtgac gccctgtttc ggggcccgac tggtacagga
     6961 gggtaaccgg ttgcattacc ttgctgaccg cgccggtatc agaggccggt tcagcgacgc
     7021 ggatgcgtac catctggacc aggcctttcc gctgctgatg aaacaactgg agctcatgct
     7081 caccagcggt gaactgaatc cccgccatca gcataccgtg acactgtatg cgaaggggct
     7141 gacctgtgaa gccgacaccc tcggctcctg tggttacgtt tatctggctg tttatccgac
     7201 gcccgaaatg aaaaattaac tctccagaat agccttctgc tacggcccgg tgttttcacc
     7261 acgccacttt tccattttta tatctgcata tcaggaaaat cttcagtatg aaaacattac
     7321 ctgtattacc cgggcaggcg gccagttctc gcccgtctcc tgttgaaatc tggcagacac
     7381 tgctcacccg actgctggac cagcactatg gccttacgct gaatgacaca ccgttcgctg
     7441 atgaacgtgt gattgagcag catattgagg ccggcatttc actgtgcgat gcagtgaact
     7501 ttcttgttga aaaatacgcg ctggtgcgta ccgaccagcc ggaattcagt gcctgtaccc
     7561 gctctcagtt aataaacagc atcgatatcc tccgggctcg ccgggtgact ggcctgatga
     7621 cccgcgacaa ttaccgaacg gtaaataaca ttaccctggg taagtatcag gaggcgaaac
     7681 gatgaaactg gccctgacgc tggaagctga cagcgttaac gtgcaggcac tgaacatggg
     7741 gcgcattgtc gttgacgtcg atggtgttaa tctcactgaa ctgattaata acgtcgctga
     7801 aaacggttat tcactccgtg tggtggagga atccgaccaa cagtcaacct gcacacctcc
     7861 gccatttgca acccttgccg gcatacgctg cagtaccgca catatcacgg aaacggacaa
     7921 cgcctggttg tactcgctgt cacaccagac cagtgacgtt ggtgaatcag aatggattca
     7981 tttcacaggt agcggctatc tgttacgtac cgatgcgtgg tcatacccgg ttctgcggct
     8041 taagcgcctg ggcctgtcaa aaacgttccg ccgtctggtt gtcacactta cccgacgtta
     8101 tggcgtcagt ctcattcatc tggatgccag tgccggatgt ctgccgggtt tacccacttt
     8161 cgactggtaa ccagaaacaa catgaaatca ttaaccacgg aaacagcgct ggatattctg
     8221 attgcatggc tgcaggacaa tatcgactgc gaatcgggaa ttatctttga caacgatgaa
     8281 gataaaacgg attcggcagc actgttgccc tgtattgaac aggccaggga ggatatccgt
     8341 accctgcgcc agctgcagct tctgcaccag aaccggtgaa tctcactcac cagacttcat
     8401 tcc
//
LOCUS       DAZKJR010000191         8276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKJR010000191 DAZKJR010000000
VERSION     DAZKJR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.192"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..1632
                     /locus_tag="ACHOWS_004670"
     CDS             1..1632
                     /locus_tag="ACHOWS_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFQ0050141.1"
                     /translation="MMSTANAADDVKLKATKTNVAFADFTPTEYSTKGKPNIIVLTMD
                     DLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAIEAAKKSTPTLAALMDEGVKFTN
                     GYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGIPLEETFLSELLQNHGYYTAAIG
                     KWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQNRGFNYFMGYHASGVSLYNSPS
                     LFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPFMIYLAYSAPHLPNDQPAPKNYQ
                     EKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQYDNTIILFTSDNGAVIDGPLPL
                     NGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKLVSSMDFYPTALDAADIKIPASL
                     KLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSHWWDEENTAFWNGYHKFVRKESD
                     DYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLALYDINDLQQTKDLSASHPDVVKN
                     MKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            1701..1958
                     /locus_tag="ACHOWS_004671"
     CDS             1701..1958
                     /locus_tag="ACHOWS_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050142.1"
                     /translation="MNKHENKKYCSSDEIWCTQRKELIKSVLETKKASDLEKQSTDIA
                     QQYLSETIQFSIDDFIKNIKRKVKSRGSRPPAKRKNTHKGT"
     gene            2179..2394
                     /locus_tag="ACHOWS_004672"
     CDS             2179..2394
                     /locus_tag="ACHOWS_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0050143.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            2568..2825
                     /locus_tag="ACHOWS_004673"
     CDS             2568..2825
                     /locus_tag="ACHOWS_004673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050144.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            2807..2998
                     /locus_tag="ACHOWS_004674"
     CDS             2807..2998
                     /locus_tag="ACHOWS_004674"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0050145.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            3140..4159
                     /locus_tag="ACHOWS_004675"
     CDS             3140..4159
                     /locus_tag="ACHOWS_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0050146.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVTTMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            4880..5095
                     /locus_tag="ACHOWS_004676"
                     /pseudo
     CDS             4880..5095
                     /locus_tag="ACHOWS_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            5270..7240
                     /locus_tag="ACHOWS_004677"
     CDS             5270..7240
                     /locus_tag="ACHOWS_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFQ0050147.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            7259..8050
                     /locus_tag="ACHOWS_004678"
     CDS             7259..8050
                     /locus_tag="ACHOWS_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFQ0050148.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         2548 a         1807 c         1904 g         2017 t
ORIGIN      
        1 atgatgagta ctgcaaatgc agcagatgat gttaagctca aagcaacaaa aaccaatgtt
       61 gcctttgcgg acttcacgcc gacggaatac agcactaaag gtaagccaaa tatcattgtt
      121 ttaacaatgg atgatttagg gtatggacaa ttgccttttg atgatagttc atttgaccct
      181 aaaacgatgg ataaccgaaa tgttgttgat acatacaaaa taggtattga taaagcaatt
      241 gaagctgcaa aaaaatccac tccaacatta gcggccctga tggacgaagg tgtaaaattc
      301 actaacggct atgttgcaca cggtgtctct ggcccttcac gtgccgcaat cctgacggcg
      361 cgagctcctg ctcgcttcgg tgtatattca aattctgatg cacaggatgg tatccctctg
      421 gaagaaacat tcctgtctga attacttcag aatcatggtt attacactgc agcaatcggt
      481 aagtggcact tatcaaaaat cagcaacgta ggtgtgcctg aagaaaaaca aacccgtgac
      541 taccacgata acttcaccac cttctccgca gaagaatggc aacctcagaa tcgtggcttc
      601 aactacttca tgggttatca cgcatccggc gtttcacttt ataactcccc gtcattgttc
      661 cgtaaccgtg aaaacattaa agctgaaggg tacattagtg atcaattaac tgatgaagca
      721 attggtgtaa ttgaccgtgc aagcaaactg gaacagccat ttatgattta tctggcatac
      781 agtgcaccac acctgcctaa cgatcagcct gcgcctaaaa actatcagga gaaattcaac
      841 actggtagca agactgctga taactattat gcgtcaattt actccgttga ccagggggtt
      901 aaaaagatcc tgaatacact taaggaaaaa ggtcagtacg acaacaccat cattctgttc
      961 acctctgata acggtgctgt aattgatggt ccactgccac tgaatggtgc gcaggctggt
     1021 tacaaaggcc aaccgtatcc gggtggtatg catacaccta tgttcatgtg gtggaatggc
     1081 aagctgaaaa cgggtgatta tgacaaactg gtttcatcaa tggacttcta tccgaccgct
     1141 ttagatgccg cagatatcaa gatccctgct tctctgaaac tagatggtgt atcattattg
     1201 ccatatgtac agaaaggagc tgataagaaa tctgcagctg gcgaacctca caaatatctg
     1261 tcctggatta cctcttattc tcactggtgg gacgaagaga acactgcatt ctggaacgga
     1321 tatcataaat tcgtgagaaa agagtctgat gattatccgc acaacccaca tacagaagac
     1381 ttaagccagt ttgcatacac tgtaagaagc aacgattatt ctctggttta cacctatgca
     1441 gagaataaac tagctctgta cgatatcaac gatctgcaac aaactaaaga tctctctgca
     1501 tcccatcctg atgtagttaa gaatatgaag gctgttatga ttgacttcat cacgtcaagt
     1561 caaaaaccga tcagtgatat caaccaagcc aaatttgaga atataaaaaa atctttaact
     1621 aacgtgaaat gataataagc agggagtaga tatatctact ccctggatat agggaaataa
     1681 atcagttaga gcaataaata atgaacaaac acgaaaataa aaaatactgt tcatctgacg
     1741 agatttggtg tacacaacgt aaggagttaa tcaaatctgt tcttgaaaca aagaaagctt
     1801 ctgatttaga aaaacagagt acagatatag ctcaacagta tttatcagag acgattcagt
     1861 tttctattga tgattttatc aaaaatataa aaagaaaagt taaatcccga ggttccaggc
     1921 ctcccgccaa acgtaaaaac actcacaagg gaacatagat tcaatgcgtt tatttagaag
     1981 gtctgtcttc tttttaatat taacttatta attggtgcta ttcatataat tctttaaaca
     2041 gcgaacttat agccaggatt caatagatat agtaacagga acatcagtaa gcgatgagat
     2101 ggtcttcccc ctccttcacg acaaccttta attacgtcat gatggataaa ctttaaccac
     2161 aaaagtaaga gggataccat gtctcaacag aaaatcaccg gcatcgatct ggctaaaacg
     2221 aacttttatc ttttcagtat caatgctcat ggcaaacccg caggaaggat caaactttct
     2281 cgcaatcaac tgcttaactg gctggtgcag cagcctaaaa tgactgttgc gatggaggct
     2341 cgcgggcatc ccatcactgg gccagggaaa tcaggaaact cgaccatgat gtaatatttc
     2401 ttccggcgca acatgttaaa gcttatcagc gacgccagaa aaatgattat gacgacgctc
     2461 aggcaatagc tgaggcctgc caacatggaa ctatacgccc cgtaccaata aaaactctgg
     2521 agtaacagga cgtccagact tttttgaaaa tgcgccggtt ggtttcgatg gaaaggacac
     2581 aactgataaa tcacgttcgt ggacttcttg ctgaatatgg cattgtgttc agtaaaggcg
     2641 caacagaatt acgccaaaaa ttaccggcat tacttgagga tgcggaaaac gaactcactg
     2701 ataccatgaa gacgttattg catcggcagt atatacggtt gattacacta gataatgaac
     2761 tggagtggta tgattctgaa ctgaaaaata tgtccgccaa gatcctatgt gcaaacgctt
     2821 gttagccatt cctagattca gccctctggt tagtcaggca ataaaaagct ggatggggga
     2881 tggaaaacag tttagacgag gaagagatgc atctgcgact ctgggactgg ttccccgaca
     2941 gttcagcaca ggtgggaagc aggttcttct gggtataaca aaatttggag aaatgtaatg
     3001 aacacatccc accccattaa gtcaggtgct ctgtgatgga tgaagggaaa tactggaagg
     3061 tgtggagcct cgcagatttc cggcatcata gtgcccaata caggatgcat aaagctccct
     3121 cagactggag actccacaaa tgaaatatac accggttggc gttgatatcg caaaacatct
     3181 gattcaggtt catttcatcg acgagcacac aggtgaagtg attgataagc aactacgtcg
     3241 tcaggatttt ctgatgttct tctgtaatcg ggaaccctgc ctgattggta tggaggcctg
     3301 tagaggctct cagcactggg ggcgtgaact gacaaagctg gggcataaag tccggttgtt
     3361 acaggcccac tttgttaagg cattcgtcat gggaaataag aatgatgtga tggatgcccg
     3421 ggctatctgg atggctgttc agcagccagg taaggacatc gcggttaaaa cagaagaaca
     3481 acagtcagtg ctggttttgc accgttcccg cagacaattg gttaagttcc gcaccatgca
     3541 gattaattct ctgcacggca cgctcctaga atttggtaaa accattcaca aaggtcgggc
     3601 agcgatggag cgagagctcc ctgaagcact ggagcggatg aaagagagac tgccgccgta
     3661 tctcattact gtgctggaaa accagtacaa ccggctgaat gagctggact cgctgataga
     3721 ggatattgaa aaaaagctta ccagcgtggc gagacagaat gaaacctgta agcggttgct
     3781 ggatattccc ggcgttgggc cacttattgc aacggcagcg gtgaccacca tgagggaagc
     3841 atcagcgttt aaatcagggc gagagttcgc ggcttatgtt ggtctggtgc ccaaacagac
     3901 tggctcagga gggaaaatcc gcctgctgga gataagcaaa cgtggtaata catatctgcg
     3961 agccttgttt attcatagcg caagggcggc agcattactg cccaaagaac ctggtccgtg
     4021 gataaccgaa ctgaaaaaac gtcgtccaac cagcgtggca atcgtcgcca tggcaaacaa
     4081 actagcacga acagtatggg cgatagcagc tcatggaagt aaatacgaca agaatcatgt
     4141 cagtatcaga tcatattaat cgctgatacc gataaatatt aacttttaat gaaaggatga
     4201 atactgaaag gtaactctgg cggccatagt gatgacaacg acaggtaaga ccgggactca
     4261 ctaaacctga atattgtgtt gagctaaaag ctcgctggag aaatgaggtg tgagtcagcg
     4321 aattacatag gggctcgcag cttcatggct gcaaaaaagc cggatataaa gctgcaacct
     4381 acccgtcact gtcaaggcaa tggatgcctt gcaacgggat gtgttcatat aatcaatatg
     4441 tcagatctat gctgatacag ggtgccagag cggtattgtt cagagcctca gagaaaactg
     4501 attcacttcg tgtgtgggtt aattgtattc gggaaaagcg tggcttcaac caggcagttg
     4561 ttgctcttgc aaataaactc cttcggatcg cctgggcaat tatcgcccgc aatgagatgt
     4621 atttgcccgc agcaaaacaa aatcaataat ctacgtaaaa tcagtaggag taaagcttac
     4681 cctgaaattg caaagaatgc agaatgatga aaaacaggtc agaccgacgt tacgaaaacc
     4741 tgcaagatga gccaggccag taaggccgag taagcgataa ggtcggagcg cgcgaatgtc
     4801 tcatcaaggc cagaagcaat aacttcatga acaaaccgga tatacgaaag taaacctgtc
     4861 cctatcatag ttgctgtact cttgcaatac gggggaagac catctcatcg cttacgaaca
     4921 attcgtgaaa acgatgaaga aagactacat cgcgtgcatg ccgaaatcta atgtaagaac
     4981 ggcattgcat aatcttgcag tggagatcgg gtattacaat gaaaaccatc cgcatagtgc
     5041 gttaggttat ctcccaccaa gggaatatca acgtcagcga ataacgtcac cttagatgca
     5101 gtagttgtca ggaaatagtg actcaagggc aggcttacca tatacactct tagtggttcg
     5161 actgggtatt agaatagcta ttaacagatt agcactgaac actgcgtaat agtggttgca
     5221 caaacaaaat gttatgttat aacattgctg tattgacata tattttcgaa tgtatatgaa
     5281 cgtaatcaga actgtcattt gtacattaat catacttccg gtggggttac aggcagcgac
     5341 aagtcattct tctatggcta aagatacaat caccgttgtc gcgacaggaa atcagaacac
     5401 ggtatttgaa acgccgtcga tggtcagtgt catcacgaat gacacgccat ggagtcagaa
     5461 tgcggtcaca tcagccggta tgctgaaagg tgttgcgggt ctcagccaga ctggtgcagg
     5521 acggaccaat gggcagacct ttaatctacg cggctatgac aaaagcgggg tacttgttct
     5581 tgttgacggc gtccgtcaac tcagtgacat ggcaaaaagc agtggcactt atctggatcc
     5641 ggcactcatc aaacgcatcg aagttgtccg cggaccaaac tccagtctgt acggcagtgg
     5701 cgggctgggg ggggtggtgg acttcagaac tgccgatgct gcagattttc ttccccccgg
     5761 agagacaaat ggtgtaagtc tgtggggaaa tatcgccagt ggtgaccaca gcacaggctc
     5821 ggggctcacc tggttcggta aaaccggaaa aacagatgcg cttctttctg tcattatgcg
     5881 taaaagagga aatatctatc agagtgatgg tgagcgtgca cctaacaagg aaaaacctgc
     5941 agccctgttt gcgaaaggct ctgtcagtat aacagacagt aacaaagcag gtgccagctt
     6001 gcgtctctac cagaataaca ccactgaacc gggcaatccc actctgacac atggtgacaa
     6061 cggcctgcgt gacagaaaaa cagcacaaaa tgacgtgcag ttctggtacc agtactcccc
     6121 tgcggataac agcctcatca atgtaaagtc aacgttatat ctcagtgata tcactatcaa
     6181 gacaaacggt cacaacaaaa cagcagaatg gcgaaacaac agaacctccg gtgttaatgt
     6241 tgtcaacagg agccatactc tgatttttcc gggagcccat cagttaagtt atggcgccga
     6301 atattaccgt cagcagcaga agccggaagg ctctgccaca ctatatccgg aaggaaacat
     6361 tgactttaca tcgttgtatt tccaggatga aatgacaatg aaaagcaacc cggttaacat
     6421 tatcgtcggt tcccgttatg accggtacaa gagcttcaat ccccgagccg gaaaactgaa
     6481 agccgaacgc ctgtccccca gggcggcgat ttcagtctca ccaacagact ggctgatgat
     6541 gtacggctcc atatcctctg cattccgtgc ccccacaatg gcagaaatgt acagggatga
     6601 tgtacatttt taccgcaagg gtaaacccaa ttactgggta cctaacctta atctgaaacc
     6661 agaaaataac attacccgtg agattggcgc aggtattcaa ctggacggcc tgcttacaga
     6721 caatgaccgg ctgcagttga aaggcggata tttcggaacg gatgccagaa actatattgc
     6781 cacgcgcgtg gatatgaaac ggatgcgttc ttattcttat aatgtatccc gggcccgtat
     6841 ctggggatgg gatatgcagg gtaattacca gtctgattat tttgactgga tgctttctta
     6901 taaccgcacg gaaagtatgg atgccagtag cagggaatgg ctgggctccg gcaatcctga
     6961 cacactcatc agtgacatca gtatacctgt tggtcatgga ggagtttctg ccggatggcg
     7021 tgctgaactt tcggcagcag ccacgcatgt gaaaaaaggc gatccccatc aggctggcta
     7081 tgccgtacat tccttttcac tgtcttataa gcctgtaagt gttaaaagct ttgaggcatc
     7141 agtgactctg gataatgcct tcaacaagct tgccatgaat ggcaaaggtg tgccgctttc
     7201 aggcagaact gtcagtcttt atacccgtta tcagtggtaa cctcgcagga aataaatcat
     7261 gatgaaaaat acaggctata tcttagctct ttgtctgaca gcatcggagc atgccctggc
     7321 ccacgatgtc tggattaccg gtaaacaggc agagaacaac gttaccgcag agattggtta
     7381 tggtcataat ttcccctcaa agggcacaat tcctgacaga aggaatttct ttgaaaatcc
     7441 ccggctttat aacgggaaag agataataac gctgaagcca acgtccacgg attatgtcta
     7501 taaaactgag tctgcagcca acggtaatag ctacgttctg tcaacgaaaa tgaaaccggg
     7561 atactggtcg agaacctcgt caggatggaa gcctgtcagt cgggagggca gaaatgatgt
     7621 ggcatactgt gagtttgtga ctaaatatgc aaaatctttt attcctggtg aacagcagat
     7681 gccagcacaa ctctatcagt ctccaacagg ggatgagctt gaaatcattc cattatccga
     7741 tatcagtcgt ttcggtgaag atgtgaagct gaaagttctg tataaaacat ccccgcttgc
     7801 cggagctact ctggagcttg actctgtcag ttatctgaaa tcatcccgtc atacccatgc
     7861 agctgagcat aaacattcgg ctcataaagc agaacttacc tttgtaagta atgaggacgg
     7921 tatcatcact gtaccttccc ttcatgtcgg gcaatggctg gctaaagtca aaaataagaa
     7981 agtatttccg gacaagagtc tgtgtgatga aactgtcgat gtggcaacct taagcttctc
     8041 ccgaaattaa taatactatt cctgccctga tttatgggca gggtattatc aatacgagaa
     8101 tagcctaccg acatccgtgt aaaaaaccac ataatgattt ttcatatcca tttccagata
     8161 tacttatgta aagatcacct ccaaaaaagc gtaatagtaa taaaaatccc tgaagcaccc
     8221 tgttcaaacc agaaggaata cataacaaga caataaaatg tgaaatccat cacagc
//
LOCUS       DAZKJR010000192         8123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKJR010000192 DAZKJR010000000
VERSION     DAZKJR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.193"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..305)
                     /locus_tag="ACHOWS_004679"
     CDS             complement(<1..305)
                     /locus_tag="ACHOWS_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050149.1"
                     /translation="MIRIIVFLLFFLSGCTSQKEYSNIVRIENRCSSDIDVFIQGYVS
                     GSTLIYQHNIHLPVGDSDVLVYFSTADIDDGFKYEGAFMIYKDSRGELDYPFTISLSG
                     "
     gene            complement(298..1704)
                     /locus_tag="ACHOWS_004680"
     CDS             complement(298..1704)
                     /locus_tag="ACHOWS_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFQ0050150.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKIKS
                     KSLLVRLIALCGDMMIKVDYPLKNAAKEEEWVDVKVDRNNKVVDITWRLSVKDSGTKG
                     SNPELSPIPYSDLVSLTKNGVEFYWSRNGSRGGGIGGNIVTAIGVFKVNVKAEINITP
                     SMRTFDLISSLDPDMQSSVSLLGFEKIYHNYGGYYNNSKDEIKALLYSNERYKWDSAH
                     EMGHKVMDKYGSESWTHKGTSTRGQSTIPNTPMPSSGEIDIMKYADSYAPDRYKRLVA
                     ADEDVQALIWLSRVSFND"
     gene            complement(1710..3320)
                     /gene="tssA"
                     /locus_tag="ACHOWS_004681"
     CDS             complement(1710..3320)
                     /gene="tssA"
                     /locus_tag="ACHOWS_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFQ0050151.1"
                     /translation="MSELLQKLTRSCFADRDALDSARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGAQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGRMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADELTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            complement(3313..6639)
                     /locus_tag="ACHOWS_004682"
     CDS             complement(3313..6639)
                     /locus_tag="ACHOWS_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFQ0050152.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELHRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSTEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLSERCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GIAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNAR
                     GRTVGFPWLRITLMSAVCVLAIWGAGMTTSFFANRALVQETSIQTARALDIRLPLSEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADDLRY
                     AGVPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDMLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGSLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWDQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            complement(6696..7910)
                     /locus_tag="ACHOWS_004683"
     CDS             complement(6696..7910)
                     /locus_tag="ACHOWS_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050153.1"
                     /translation="MPIRLNTIPDAEPYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVGSVRWLVFVTQHIRADAWNQRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARHQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTVELNREQRASLLSS
                     NQPFTQEALMYELDAFLGCSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQNGHYSWAT
                     MVSPFVSPQEAS"
BASE COUNT         1725 a         2384 c         2098 g         1916 t
ORIGIN      
        1 cctgacaatg aaatggtgaa tggataatcc aattcaccgc ggctatcttt atatatcatg
       61 aatgccccct catatttaaa accatcatca atatctgccg tggaaaaata tactaataca
      121 tcactatcac caactggcaa gtgtatatta tgctgataaa tcagcgtaga accggaaaca
      181 tatccctgaa taaagacatc gatatctgag gagcatctat tctcaatcct gactatattg
      241 gaatactctt tttgtgaagt acacccactc aaaaaaaaca ataaaaaaac aattattcta
      301 atcattaaaa ctcaccctgc tcaaccagat caatgcctga acatcttcat ctgccgcgac
      361 caatctctta tatctatcag gtgcatagct atctgcatat ttcattatat caatctctcc
      421 tgaagatggc attggagtat tagggatggt actttgaccc cgcgtggatg tccccttatg
      481 tgtccatgat tcagaaccat atttatccat aactttatgt cccatctcat gagcactatc
      541 ccatttatat ctttcattac tatatagaag agcctttatt tcatccttgc tgttattata
      601 atagccacca tagttatggt atattttttc aaaccccaaa agacttaccg acgactgcat
      661 atctggatct aacgatgata tcaaatcaaa agttcgcata gatggagtaa tatttatctc
      721 agcctttaca tttactttaa aaaccccaat cgctgtaacg atattaccac caattccccc
      781 gccgcgactt ccatttcgtg accagtaaaa ctccacgcca tttttagtaa gactaactaa
      841 atcgctataa ggtattggcg ataactcagg gttactgcct tttgtaccgc tatccttaac
      901 agataatctc catgttatat cgaccacttt attatttctg tcaactttta catcaaccca
      961 ctcttcttct ttcgcagcat ttttcaatgg ataatcaact tttatcatca tatcaccaca
     1021 taaagcaatc agtcttacga gtagtgattt gctttttatc ttagttgtat ccagtatgcc
     1081 ttgattactg tagccatccc attgccaggc atgctctccc ttatcacaat atttaccggt
     1141 ctgccgttca ctataaatca cttcattacc atcaagcact tccagagaaa gcgcttcagc
     1201 ctcatcgcct ttatttgtga tggtaaaatc tattttttct gtattcttcg tactaccacc
     1261 gctaatcatt tctttataat caggtatatt cagtggcgtc agtatcttat tacctgataa
     1321 tttaattctg taatccagtt ttacatcatc agaggatgaa tcagaaccag aagtcagatc
     1381 ctttttcgcc gactgcgcat gttgttccgc ttcactttcc ccgtcatccg tctcatacgt
     1441 atcatccatc attgacgcaa taaaccgtgc cttacacggg caactgctct gactgtccag
     1501 cgtccccgcc atcagcctgc cgtgaatatc tgtttcagga acgccgccca caataataaa
     1561 cattcccgga tatctgccgc aggtgacccg gtccatatcc cgcgcctgcg ggatacccat
     1621 gatcgtatgg tcgtccgcgc cttcaataat tttgccgccg caggtggttt tgtcccccct
     1681 gaccagaaag tagcctgttc ccattgccat cagtccttat gttgcgtatc acacaatacc
     1741 gctgcccgta ccggatctat tgtcaccagc gttcccagca ggatttccat ttttcggttc
     1801 agcagggctt tatccgcata gcggtgcgcc cgcaggcgca gcagtttcag ctgtcgggct
     1861 tttacctcaa acagcaggtc cggctcccac tgcgtcagcg tcagcccctg tgcggcgata
     1921 tccagttctt ccagcagcag gagcgccatc tcgtttttac cgtactgttc cgccacccgc
     1981 gccatcagca gtcgcaataa caggcgctgg cgtgccgtgg tgatgcccgg acgggtctgt
     2041 atccagccca gcgccgcttc cacgccctga ctgtctgcca tctccagcgc ttccggctcc
     2101 agcgccagta cgtcgtttgt ggcgtcgtct gtcggtacag cggctttccc cgcaagccat
     2161 gcgccgtctt cccgcatcat gacctgctgc gctatccagt tgcgcgtcaa ctcatcggcg
     2221 aacggcgtgc cgtcgttcca tgccagattt tccagccccg gcaggcgttc cagtagtaac
     2281 gccaggtcct gacggataac cgctgtccac ctgtcccacg gtgcgcccgc cttagtcagc
     2341 gcctgatgca gataccactg aatgtccagc cagaaatggc tgacgccggt gctgaacatc
     2401 aggtcggcct gctccagcag ttccgtccag ttctgctgag catacagccg cttaagctgg
     2461 ttgcgtgatt ctgtgcgcgg cggggccagc cgggtttttc cgtccacatc cggcggcagt
     2521 tcatgcaccg tgtcccagcg cagggttttc atcagccggt gtgctgaaag ccagccctgc
     2581 ggctgttcgt tcagataacg ggccagcact ttcgcctggt cgagcaggtc gcgcccggaa
     2641 gtgatggtct gtacctgtgg cgcatccacc ggggaagacg gtgccggaac gccgctgctg
     2701 ttctgcggca ccaccgcgtt catcctaccc gactgcgcca gacgtgattc cagcgcgtta
     2761 atcagcggca tcagggaggg ggaatgctgg tcttcgggcc atgcagtgaa ggaaaccgtc
     2821 agttgattaa gagcggcaac aatgttcgca aaatcctctt ttgccacttc cgggtagcgg
     2881 gcaaggctgt cgagcatctt ttcccctgcc agccactcca gcgccatttt tcggcttgcc
     2941 gggcgcgagg gcagcagttg cgcaccgaag cgttccacca gtccggctaa cagcgccagc
     3001 ccctcagcca gtccgcgttc gccatcgcgg tgcagtcgcg cccagatata gtaagtggcg
     3061 atgcgcacgt cttttgcctg cgtcagcagc agggattcgg caagctggca gataaggtcc
     3121 gtatccgcgc cggaaagttt gttcatctcg tcgcggatac gcaggaaatc gtcgtggtag
     3181 ccggggtctt ccccgaccgg cgtatctgcc gtcaccggca gcagccatgt ctgccacagc
     3241 gccgcctgtg ttcgcgccga gtccagtgcg tcccggtcgg caaaacagct acgggtcagc
     3301 ttttgcaaca gttcactcat cggcgtcttc cccgtattct tccgcatcct tcatactcct
     3361 gccggtcagg aacacctgtc ccggcaggcg gaagtttttc agttccagca gcgccagcgg
     3421 ccctttaccc tgttcaaccc gcagcaggta attcagcggc aggccgtcct gcgctttcca
     3481 caccacttta aaggtactgc tgtccaccgg tgtcacctgc gcctgttcca gcaggcgaat
     3541 aaggctccag cttccccgat aatccgccag tatgcgttcc atcgcctgcg tactggtcca
     3601 gctcagcgat gcgccgggtt gccactgttc atccggccag ttaaaacgct gccagcgttc
     3661 cttctggttg aaatattcca gctcctgccc gtcaatcacc agatgcgtct tcatcacgtc
     3721 gcgcgccggt tttgcccgca gttcaaaatg tatccctgca tcgccgctgg caaaggcggt
     3781 atcggccaca tcgcgcaggc ggttcagggc gcgaataaag tccggattga ctgtcagccc
     3841 ctgcgtgttc atggcgtcag ccacccagta gcggccttca cgtttcagta cgccggaaag
     3901 attggcggcg ataaactggt tgatgcgccc gtcatcgcgc aggtactgcg ccagcagggg
     3961 gagggaggca tcgttttgtg aggctttgaa cgggtagcga ccagcgaagt cctgatccca
     4021 gtggctgaca atcgcccgct gccactggcg gttcaggctg tctgccgcag gcgtcagcac
     4081 ctgccgccat gactgctcca ccgggcggac gaacagcgcc tgaccgaagc cactccactc
     4141 ttcacccaga ctggcggcta tcaggctgcc atagtcgcgg gtgtcggtaa ggtcaacggt
     4201 tttgccttgt aagaccgtct gcgccagttg ctgggtcatc tcctgcgggt caggggcgtt
     4261 ggtcacctgt tgcagtttga ggcgcacgcg ggtgactcgg gtgaggtagg tctgcaggct
     4321 cagctggctg tcgccgcctc ctgccttatc gccaagcaac cgcagcagcg gcgcgaaggt
     4381 tttatccagc ggcccgacag gcacgtcgcc ttcacgggat tgctgcggcg ttttctcctt
     4441 gccgttaaac agttcctgtg ccgatttcgc cagcgagtct gacaggccac ggttttccct
     4501 gccagccgcc gcctgccacg cgagggtgtc tgtcagcgca atcagcggtg actggcgggc
     4561 atcggccatc agcgtcaact ggtcgagaat gccggagagc gagtcttcct ttttccagcg
     4621 aatgctgttg agaaacgcca gccagcttcc ggcaaagtcg gtaaagtagc gtgaagtgag
     4681 acggttacgc agcatatccg gcgagatatc cgcagaggta tcctgctggc ggtcgctgag
     4741 tacccagtcg atttcctcgc gccgcgccgt caccacctgc tcgatggctt ccctgacctg
     4801 tccttcccac gcctgacggg taaacatccc cggcaccgtc tgctccgtac tgaaaagaga
     4861 ttcggtgagc gtatccccgg tcatgtccgc cagcgtcata tcggcgtagt tgcgggacac
     4921 ctgttgcagt acgttctggt agagagtgtt ttcggcattg cgcacaccaa tctggcgcag
     4981 caggattttg cgcactgcgc cggtcagttc cggtggcggc gaagttttcc actgtgggtg
     5041 ttccggcaga ttcgccatcc agaaggtcag cagtgacggc aacacgctct gacgcacacc
     5101 ttccggtaca cctgcatagc gcaggtcatc tgccatcagc gtggtactga aaaaggcagc
     5161 gtcggccttt tccgggcggg aagtcatcag caatgcctta agctgcttat agcgctgttc
     5221 gccggtggcg gtacgctgcg gactattggg cggcagcgcg acaaaggcat tcagttgctg
     5281 ttgcagatgg tcaacggcca cgtcgcgcac cagccggttt gccgcctgcg catagccggg
     5341 aaaggcggcg gcgagcagtt gctggttacg ttcaaggcca aaacgctgat accacggcgc
     5401 accctcccgg atacggtact gcaggcgctc cagttcaccc tgcagggtat gcagcgccac
     5461 cagttgttca gacagcggca gacggatatc gagtgcacgc gccgtctgga tgctggtttc
     5521 ctgaacaagt gcgcggttgg cgaagaacga ggtcgtcatt ccggcccccc atatcgccag
     5581 tacgcagaca gcagacatca gcgtgatacg cagccacgga aagcccaccg tgcgaccccg
     5641 cgcgttatcg ccggtcacgc ccgcccagac cggtgacggc agccacgcat gaggtgcggc
     5701 ttccggtacg gcggtaagcg gcgggctgaa catcaggcca cgcaggcgca gggaggaaaa
     5761 tgcgccgcct gccagcagcg gtttcaggat actttgccag tgagcgatac cccgctctgc
     5821 aagggtatgg gccagacgca gcaggaaatc atggcggtta tcggtaagca gctgcgacat
     5881 gccctgttcc gtcagcggca gggtttgtgc ttccagcatt gcagccagtt gttccggggt
     5941 acagcgttcc gacagcaggc agccgacgct ttgcaggggg cgtccggtct gataatcacc
     6001 gttgtcacag acctgccaca aatagagtgg agcctgaaaa ccgagtcgct taccgccgtt
     6061 tatcagttcg cgccagcctt tgtcgagttg cgccgtctgg cggctctgtt cttctgtcag
     6121 cggccagatg atgccgtcca gcggacggct gcggcgcagt ttttttaagg cggtcagtag
     6181 cgcgacatca ggctctgtag atggcctgcc gccatatatc agcaccgtgt ggtcgccctc
     6241 cagccagtgt tgcccggtca gccccggcgc gatggcctct gcctgttcag gctcgccggt
     6301 caccagcagc aggcggattt tacgccgcca gaatgggccg tagcggtcgc gcaggaaggc
     6361 ggcgagttcc accattgccg gatgacggct gtcggctttc tttgtcgtca ccgcccgata
     6421 gtcatctccg gcggcaagct gtctgtggag ttcaaagctc tgtacggcag cgtggcggtt
     6481 cagcagatga taaaccccca tgataatcag aaagaccaca cctgcggcaa tcacgccagc
     6541 catccagtac atttgctgtg aactacccgg ttcaacgccc agcaatgccg gtttacggtg
     6601 aataaacgtg caaagcgcaa ccacaacggc cacggtcatg acgatggcag gccctgaaac
     6661 cgaacggggc cggagacggt ttaatcccgg aaccactatg atgcctcctg aggggaaacg
     6721 aatggcgaca ccatggtcgc ccatgaataa tgcccgttct gaacgccgct gagcgtcagt
     6781 tgggggtgtt gtgattgtat tgccgccagc gttgccgcag cgacggacag ccacggcgct
     6841 gccggtccgg agcaccccag aaaggcatcc agctcgtaca tcagggcttc ctgagtaaac
     6901 ggctggttag atgacagcag cgaggcgcgc tgttccctgt tcagttcgac tgtccacatg
     6961 ccggatatgg catctggctg caccggcatc cagtccagcg cctgtgcaat gcctgacgcc
     7021 atcatctccg tatctgtgat acgttcagga cgatgcagca gggccagcgg cgtcagcgcg
     7081 gtttgcgtca gtcggtttgc cagcaacagc gcggtggcgg attctgccgt ccgttccggg
     7141 ttcttcggtt ccagacgcac ggaaatcacc agcagtacgg ccttttcctg aataaagttg
     7201 tccagccagt cgtccacggc ggccagcccc ggctccgggg cagaaaacag acgggcttcg
     7261 gggagtccgg ctttttgcca ttcgtcctgc cagatagttc tcacggaggc ccctgaaagc
     7321 ggcgcatcaa tttccagtag tacgttgata tgaaaatcag ggggcagcag aaacaattgc
     7381 ggtctgatat caataaaaag gcgggaaata atttcggata aacgcccggt aaccggttta
     7441 ttggttaata cgggaatgac actgcgacgc acggtatctt ctccgcgcaa atcagcgtaa
     7501 gtattcaaca cctgatgacg cgccagaaat gctgttgtag tttctgcgac ggaatcacca
     7561 ttcagcgcgg tttgtacgga aaaactgagt atctgcaacg cccgtcgtcc ccgccgtgtt
     7621 tcctgcaaaa tgacttcttc gcgacgttga ttccaggcat cggcacgaat atgttgagtg
     7681 acaaatacca gccagcgcac cgaaccgacc agtgaccaga taaaagcagg cacgccgcag
     7741 gccagccccc agaaatatgg gccgtttttt gccagttcat cgctggcgaa taatgaggtt
     7801 attgccacac caacagacag catcagtaat aaggctgaca gccatttaat gcgggctggc
     7861 ggtgcgggat agggttctgc gtcgggaatg gtattaagtc ggatgggcat tgtaataatc
     7921 cgttattctg aaatatgaat acaatgaatc tcaaggtgct tgaactatct gattaattca
     7981 cttgtccata atgtttttgc tttattaact gtgatttacc tcatcccatc atatggtaat
     8041 aaaaagaaat tatcgcatgc ttttttattg catccccagt aaatctcttt atcattgctc
     8101 atccacattg ctgttgcagc agc
//
LOCUS       DAZKJR010000193         7890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKJR010000193 DAZKJR010000000
VERSION     DAZKJR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.194"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..682)
                     /gene="zinT"
                     /locus_tag="ACHOWS_004684"
     CDS             complement(32..682)
                     /gene="zinT"
                     /locus_tag="ACHOWS_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFQ0050154.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(939..1574)
                     /gene="msrQ"
                     /locus_tag="ACHOWS_004685"
     CDS             complement(939..1574)
                     /gene="msrQ"
                     /locus_tag="ACHOWS_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFQ0050155.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
     gene            complement(1575..2579)
                     /gene="msrP"
                     /locus_tag="ACHOWS_004686"
     CDS             complement(1575..2579)
                     /gene="msrP"
                     /locus_tag="ACHOWS_004686"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFQ0050156.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTHERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(2688..3101)
                     /gene="hiuH"
                     /locus_tag="ACHOWS_004687"
     CDS             complement(2688..3101)
                     /gene="hiuH"
                     /locus_tag="ACHOWS_004687"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFQ0050157.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3234..3905
                     /gene="hprR"
                     /locus_tag="ACHOWS_004688"
     CDS             3234..3905
                     /gene="hprR"
                     /locus_tag="ACHOWS_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFQ0050158.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3905..5263
                     /gene="hprS"
                     /locus_tag="ACHOWS_004689"
     CDS             3905..5263
                     /gene="hprS"
                     /locus_tag="ACHOWS_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFQ0050159.1"
                     /translation="MKKLSITVRLTLLFILVLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGHGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICL
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(5370..6221)
                     /gene="hchA"
                     /locus_tag="ACHOWS_004690"
     CDS             complement(5370..6221)
                     /gene="hchA"
                     /locus_tag="ACHOWS_004690"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFQ0050160.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(6813..7886)
                     /locus_tag="ACHOWS_004691"
     CDS             complement(6813..7886)
                     /locus_tag="ACHOWS_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFQ0050161.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRDVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         2192 a         1660 c         1813 g         2225 t
ORIGIN      
        1 ctgttaaata actgaagtca gcgcaaaaaa atcaatgaga catcatctcc tcgaccactt
       61 cttcgctact caactgatag ggataatacg ttggccagtt ttccatttca ttcaataaag
      121 attgctggga atcattaccc ataaaaatgt gaaaatgaga tgattttcgt ggtgcaatta
      181 tatgatcgct aaattgtata tatttagggg ctttgctttc aggatcttta cattcgaata
      241 agtaacgaac gcctttcttg cctgatttat aggtgagtat tttgtatcca tcgtaatcat
      301 atttacagga tgttgtttca ttatttctat ggaattcaac aatgccgtcc tcaatgccaa
      361 tcatctcgat atctgttgca taacctttgt gataataatc tttaatttca gcaaatgttt
      421 tagttttatc tgcatccgct ttcttctgaa agacggggtc aagtttgcca ctttgcaata
      481 aaggataaac ggattgccag actccatccc agtcacttag cgttcggttt tgtacattgg
      541 catcatcaaa aacaccatta gcagcttttt gttcgacctc tgttaagggt ttgccgtgtg
      601 agtgatgacc atgcgaaaag gcaggagcgc taacaataaa gacacctaaa gcaacagcca
      661 gtttatgaag acgaatcgcc aaaacagttt cctccagagt taatgtatat gatgtgtaat
      721 gttatattgt aacatataac aaaatgcaac aactcttagg agacaagaga atagttctct
      781 caggtgtgct tatctatctt attgaaataa aataaattat gtcgatgatt tgaggttgat
      841 gacaaacgaa acataaccta atgcgatctg tgtatgccgg ataaggcgct cgcgccgcat
      901 ccggcaggga taacgcgtac tttgtcggca atatcaagtt atagagataa tttgttgtga
      961 acctgcttgc gtaaccggtt gaaaagggaa agcaacttct tataccgtaa ggctaaaagc
     1021 agtaccgcca gcccagcgta gatgagtggc tgcggtgaaa taatcttcac tgaccacaga
     1081 taatgtattg gggccaggat cgcgacaaga tagacgaaat tatgcaactg ttgccagtgc
     1141 ttgccgagtt ttcgctgcat cgcctgggtt gaagtaaacg ctaaagcaag caaaataacc
     1201 cagctgataa ttcctaacgt taaataaggc cgggtaatta actccttacc taataacgcc
     1261 agattattca cgcccagctc cagtaatgcg taactggtta aatgcagtgt cgcccaggca
     1321 aagcaccata agcctaacag gcggcgagtg cgtatcaata acggctgttt tgcgtagcgt
     1381 gccagaggtg tgattaacaa ggccgcaagc aaaaatttca gtgcagtgcg accggtaaaa
     1441 tgctgaatat ctttcaccgg atcggcaccc aatccaccgt gattaatcgc ccagaccagc
     1501 cagagaaacg gcaacaatcc ggcaagatgc aggcaaactt tcagccatgt cacctgtttt
     1561 gctgtcagac gcatttagaa attctcccgc aaatccaggc cacgatacag cgatgccacc
     1621 tgctcggcgt aaccattaaa cagtagcgtt ggctggcgct gtacatcgag gatgccgcct
     1681 gaaccaataa atcgttcggt agcctgtgac cagcgcgggt gatcaacatg cggattaacg
     1741 ttggcgtaaa aaccgtattc gtcaggcgct gccagattcc aggtggttgg cggacgctcg
     1801 tgggtcagct taatactgac gatcgattta atccctttaa agccatattt ccacggcaca
     1861 atcagtcgca ccggcgcgcc attttgtggc ggtaacgcct tgccataaac acctacggtc
     1921 atcagtgtga gcggatgcat tgcttcgtcg agacgcaatc cttcgacata aggatatttc
     1981 agcccgccgc cgataaagcg gtcctgctgg cctggcatct gttccggtgc ataaattgtt
     2041 tcgaaagcga catacttcgc attgctggtg ggttcggcaa gcgccagcaa tttgtgcagc
     2101 ggaaaaccaa tccacggcac caccatcgac catgcttcca cgcagcgcat acgataaata
     2161 cgctcttcca gcgggaagcg acgggttaaa tcatcgtgat cgagggttaa tggttttgct
     2221 acttcgccgc tgattttcag tgtccatgga tcggttttca ggctaccagc attagcggcg
     2281 ggatcggctt tatccagccc gaattcatag aagttgttat aaccggaaac tttatctgct
     2341 ggcgtcagtg gcaggttatt ttgccaggcg gcaggcttgc tgaactccag cggttttccg
     2401 gcgggggcgg gtggacgatc gttcccttta aaccagctaa gcagatcggc atgcgcagcg
     2461 tgaggcaaag atagtgcagc cgcgctgatg cccagtgctt ttaacacctg ccgacgcttc
     2521 ataaagaata ccgactcggc cgtaacatct gattctttta aaaattgatt ctttttcata
     2581 agccatcaca cttttttgtc gttagccagc aagagccgtt tactgttaat ctgtcaggga
     2641 gtcacctccc taacagtagc agagatctgc ttttatatat ttatgcatta actgccacga
     2701 taggttgaat acccatattg gctaagtaat aaaggcacat gataatgctc gttcacttta
     2761 ttaatatgaa attcaacggg gatctcaggg aagaaacttt caagattttg tttcttgaaa
     2821 tagtccccgg ttttaaatac gacacggtaa tcgcccgtag tcgcagtttg ctcgggccac
     2881 agtgccttaa ttcgtccatc cttatctgtt ttggcggtat taagttgtaa ccagccgtta
     2941 tccgctttct tttcaagagt gactgtcacg tcggcagcgg gttttcctgt ttgctggttc
     3001 aaaatgtgca cgctaagaat gttttgttgt gcagcataga ccaaagaagg taatgaaaat
     3061 gcagccgtta ctacggagag tactaaataa cgctttaaca tgtttatatc cttgtcatgt
     3121 gaatgagttt tgaggatagc gaaaaagcgt ccagggtaac cttacagcag cattacaatt
     3181 ttgtaatgaa ccaggctgtt tctataacat atgatttatg gcatattatt ttcatgaaga
     3241 ttttacttat tgaagataat caaaggaccc aggagtgggt aacgcagggg ctttccgaag
     3301 cgggttatgt cattgatgcc gtttctgatg gcagagatgg gctttatctt gcgctgaagg
     3361 atgattatgc attgatcatt ctggatatta tgcttccggg tatggatggc tggcagatct
     3421 tacaaacgtt aagaacggca aagcaaactc ctgttatttg ccttacagca agggattctg
     3481 tcgatgacag agtcagaggg ctggacagtg gagcaaatga ttatctggta aaaccttttt
     3541 cattttctga gttgctggca agggttcggg cacaattaag gcaacatcac gctttgaatt
     3601 caacattaga aatcagtggc ttaagaatgg actctgttag tcaaagtgtg agcaggaaca
     3661 atatcagtat tacactgacg cgcaaggagt ttcagttact ttggctactg gcctccagag
     3721 ctggcgaaat tatacccaga acggttattg cgagtgaaat ttggggaatc aactttgata
     3781 gtgataccaa tacggtggat gtcgccattc gcaggctccg cgcaaaagtt gatgatcctt
     3841 ttcctgaaaa gctcattgcc acaatccgtg ggatgggata ttcattcgta gcggtaaaaa
     3901 aataatgaaa aaattatcta taaccgtccg tttaaccttg ctttttatat tggtgctgtc
     3961 tgttgctggc gctggaattg tatggactct ctataatggc ctggcaagtg agttgaaatg
     4021 gcgcgatgat acaacactca ttaaccggac agcgcagatc aagcagttgt taattgatgg
     4081 ggtaaatcca gatacgttac ctgtctattt taaccggatg atggatgtta gtcaggatat
     4141 cttgatcatt catggtcatg gcatcaataa aattgttaac cggacaaatg tcagtgatgg
     4201 catgttaaat aacatacctg ctagtgagac aatcagcgca gctggcattt acagaagcat
     4261 tattaatgac acagagatag atgctttacg aattaatatt gatgaagttt cgccatcatt
     4321 aacggttact gtggctaaat tggcttcagc cagacataac atgcttgaac agtataaaat
     4381 taatagcatt ataatttgca ttgtcgccat tgtactttgc tcagtattaa gtccgctgtt
     4441 aatcagaacg gggttacgag agatcaaaaa gttgagtggt gtaacggaag cgctgaatta
     4501 taacgataac cgggagcctg ttgaggttag cgcattaccg agagaactaa aacctcttgg
     4561 gcaggcgttg aataaaatgc atcatgcttt agtcaaagat tttgagcgtc taagtcagtt
     4621 tgctgacgat ctcgctcatg aacttagaac accaattaat gcattactgg gtcagaatca
     4681 ggttacgctc agtcaaacca gaagtatcgc tgaatatcaa aaaacaattg ccggaaacat
     4741 tgaagagctg gagaatattt cgcggttaac agagaacata ctgtttcttg ccagggcaga
     4801 taaaaacaat gttttggtga aactggattc gctttctctc aataaggaag tcgaaaattt
     4861 gttggactat cttgaatacc tttcagacga gaaagagatt tgccttaagg tcgagtgcaa
     4921 tcagcaaatc tttgcggata aaattttact gcaacgaatg ttatcgaatc ttattgttaa
     4981 cgctattcga tattcaccag aaaaatcgcg cattcagata accagttttc ttgataccaa
     5041 cggctctctt aatattgata tcgccagtcc tggaacgaaa attcatgagc ctgaaaaact
     5101 cttccgtaga ttttggcggg gagataattc gcgtcattcc gtaggtcagg gacttggtct
     5161 ttctttagtc aaagcgattg ccgaattgca tgggggaagt gctacgtatc actatctcaa
     5221 taagcataac gtgttccgaa ttacgttacc gcaaagaaat taatataggg cttcagtacg
     5281 ccaaaattaa ccgtgaaaaa acgcagtcac gtcgtgagta ttaacgtggc tgcgattgat
     5341 aatgcgctta cattacaatg taacagggat taacccgcgt aagctgccag catttcctgc
     5401 gccgccagtt tacccaacgc attcgctgcg aaaggactgt cgccggtgag aactttacgg
     5461 tccttatgta ctcgcccggt gatgtcgtca ttaatgatgt tcatgcccat tttcttcagc
     5521 tcttcgccga agtaccaggt gagatgaccc ggcatatagc ctatctctgg cgtttgtttg
     5581 tctgcggcgt ctgggaatgc gcaaatggaa taaccattca gtgggttatc gccgtggcga
     5641 agcgccagaa aagccgccgg gccgtggcaa agggagataa caaaacggtc atttttgatt
     5701 gcccactgta aagcggcagc tacgtcctgg ctttcaggta aaccaataag tgcgccatga
     5761 ccaccaggaa caaagattgc tgcatattcg ctatcagcgt tgaggcttgc gacaacatcc
     5821 gcgagtttct tcggattgcg gaacaacgat ttatgctgct caaagaatgg cattactttt
     5881 tcatctttgt gtggcatagc ccagtattca aacttggtca tcagaccgga aatggtcgcc
     5941 acttcgaatt cgaaacctgc agcatggaga tgatacaacg gcagcaacgt ttcaatcgga
     6001 tggttaccgg tcgagaatag tttcccgtta tcggtcggca aataacgttc gtccgcggcg
     6061 atcaccagaa ttttatgttt accgcgatac ggttttggat agtccacgcc atcaagatca
     6121 gagacgggac tggtatattg gctaagcgaa tattctgaag ggaagaatgc attatcttca
     6181 gcaatatcga cctgcgggtt tttacttgtt tgaacagtca tagtgtattc cttattgttg
     6241 cttagttagg gtagtcacta tatttgcgac tgagctggtc gctattttta accaggctga
     6301 aaaattgagt caatatatcg aaagcggtag ttggcgggca taagtatgta atgatgatgc
     6361 tgcactggtg ttgaatatta ttcacgccaa attttcctta ttgagctacg tcgtcgcaag
     6421 tggcctcaat gattttagac cattatcaga ggtccggggc ggcagggaga gatttagtgc
     6481 tgtggaatat tgtggtgctg atgaatattt atatcgcgga ttggtagaga aaaagctttt
     6541 ttcatgcact atttttgcaa atatttctat atggacggcg gttttgttgc ggattaattc
     6601 ataaagaaat gcatttggat gttggccgga agagacgctg gtagatttta ttgagccagg
     6661 aggggtgggg taaggtgctg tatcgatatg ctttggcaat tgcgtgtgcc gcagatggag
     6721 atgaaatgcc aataaaaaag cctgagttcg atgagggacg aactcaggct ggtgttaagt
     6781 tttacagtaa cacgccgcgg cgcgttggct gattagaact ggtagacgat accaactgca
     6841 aactggtcat cagttgcggc atcggtaatc gctgcatcgt ctttatccag caggttaaat
     6901 ttgtatgctg tgtagacgtt catgttctta ttgaagtagt accaggtacc aacttcgata
     6961 taatttacgc ggtcagcatc aacaccttta tgtccatcac gagcataaag atctttgcct
     7021 ttggaatata cgtagcctaa agacggacgt aaaccgaagt cgaactgata ctgtacaaca
     7081 gcttcaaagt tttgggtttt atttgcaaaa tgatcattag ccaaaacggt catgttgcgg
     7141 gtttcaccgt acatcattgc tgcatagata ttatttgcat catacttggc tgctaatgac
     7201 cacacttcag cgttatcacc gtagccgtca ccagcttgtt cattagtacg atcagatttg
     7261 ctatatgcac ctacaaaacc aaaaccttcg atgttgtagt ctacagacaa cgcatagcca
     7321 tcaccgtttt gtttcgtaac gtcacgattg ctgttcttac cttgatattg cacagcaaaa
     7381 ttcagaccat caaccagacc aaagaaatca gagttacggt aggttgcaac accgttggta
     7441 cgaccgttca tgaagttgtc agcggacgcc caggaatcac caccccattc aactaacata
     7501 tcggtataag ctgcggcatc ataagcaaca ccgtagttac gaccatagtc gaaagatcct
     7561 aactcgttat atttcaaacc tgcgaaggtt aaacgggttt tttcctgatt agagccttcg
     7621 ggcttgctgg cgtcgaggtt gtattcaaac tgaccaaaac cagtcagttc gctggtgatt
     7681 tgggtttcac ctttaacacc gaaacgagca taggaggtat cttcgttttc gctgttatta
     7741 tcatcagtat ttgaccagat gcgttcgcca accatcttgc cgtagaaatc aactttattg
     7801 ccatctttat tataaatttc agccgcattt gctgcgccag caactaataa cgccgggacc
     7861 agcattgcca gaacttttct tttcattatg
//
LOCUS       DAZKJR010000194         7857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKJR010000194 DAZKJR010000000
VERSION     DAZKJR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.195"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..1008
                     /locus_tag="ACHOWS_004692"
     CDS             25..1008
                     /locus_tag="ACHOWS_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFQ0050162.1"
                     /translation="MPEKYSPDDQSSTIDPYLITSVRQTLEEQGAALQNLSKQLDSGQ
                     YQCVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PCDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1080..2228
                     /locus_tag="ACHOWS_004693"
     CDS             1080..2228
                     /locus_tag="ACHOWS_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFQ0050163.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLSDIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2252..3928
                     /locus_tag="ACHOWS_004694"
     CDS             2252..3928
                     /locus_tag="ACHOWS_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFQ0050164.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDISADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            3938..4678
                     /gene="kdsB"
                     /locus_tag="ACHOWS_004695"
     CDS             3938..4678
                     /gene="kdsB"
                     /locus_tag="ACHOWS_004695"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFQ0050165.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4675..6702
                     /locus_tag="ACHOWS_004696"
     CDS             4675..6702
                     /locus_tag="ACHOWS_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFQ0050166.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLSVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISKAREAMHTIVTGDLSKYNLAPAFVADESECSDIVLVVDQTFND
                     MSVTYGNAGQNEFSAMLEAAMAENPQATIWVKVHPDVLKGKKTGYFADLRATQRVRLI
                     TENISPQSLLRHISRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSVTLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLLRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWQVEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPSLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKGKKVILVPGQVEDDSSIKTGTISIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTFDEHHCGRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTSRKPMFITRKKAGWVIRYYRKLIMFCKVRFG"
     gene            6737..>7857
                     /locus_tag="ACHOWS_004697"
     CDS             6737..>7857
                     /locus_tag="ACHOWS_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFQ0050167.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRSYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPGLLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFEK"
BASE COUNT         2016 a         1880 c         2040 g         1921 t
ORIGIN      
        1 aaccttatcg gaagtataat ttatatgcca gaaaaatatt cacctgatga tcagagcagt
       61 actattgatc catatttaat tacttctgtt cgtcagacct tggaagaaca aggtgcagca
      121 ttacaaaact tgtctaaaca actggattcc gggcagtacc agtgtgtcct taatttgata
      181 atgaactgta aagggcacgt tattctttcg ggaatgggta aatcagggca tgtcggtcgt
      241 aaaatgtcag cgacgctggc ctctaccggt accccgagtt tctttattca tcctgcagaa
      301 gcgttccatg gcgatctggg tatgattacg ccctgcgatc ttttgatcct tatttctgcc
      361 agcggtgaaa cggatgaaat cctcaagcta gttccttcac tgaaaaattt cggcaaccga
      421 attatcgcca ttaccaataa tggaaattcc acgctggcga aaaatgctga tgccgtgctg
      481 gaactccata tggcgaatga aacctgcccg aataatcttg caccaacaac gtctaccacc
      541 ctgaccatgg cgatcggcga tgcgctggcg attgccatga tccaccaacg caaatttatg
      601 ccgaatgatt ttgcgcgcta tcacccgggc ggttcattag gtcgtcgcct gctgacccgc
      661 gttgctgatg tcatgcagca tgatgttcct gcggtacagc tggatgcgtc atttaaaacc
      721 gtgattcaac gtatcaccag cggatgccag ggaatggtga tggtagaaga cgcagaaggt
      781 gggctggcgg gcattataac cgacggtgac ctgcgtcgct ttatggaaaa agaggattct
      841 ctgacatccg ctacggctgc gcagatgatg acacgtgaac cgctgacgct accggaagac
      901 accatgatca ttgaagcgga agaaaaaatg caaaagcacc gcgtctcaac gttattggtg
      961 accaacaagg caaataaagt cactggcctt gtgcgcattt tcgactaatt aagcaacggg
     1021 gtttgagagg ttagtctctc attgagtttg tgccccactg gaactttgat aatcgttaca
     1081 tgttgataaa agtgaagtct gccgtatcct ggatgcgtgc tcatctgtct gccatctcac
     1141 tgtcagatat ccaaaaacac ctggtgaaaa tcatcattct ggcaccgatg gcggtgctgc
     1201 tgatctatct ggctatcttc agccagcctc gctatatgag cgagtcgaaa gtcgccatta
     1261 aacgctcgga tgatttaaac agcggcagcc tgaattttgg tctgcttctg ggtgcctcta
     1321 accccagttc cgcagaagat gcgttgtatc tgaaagagta catcaactcg ccggatatgc
     1381 tggcggcgct ggataaacaa ctaaattttc gtgaagcgtt cagccacagc gggctcgatt
     1441 ttcttaatca tcttagcaag gatgaaaccg cagaaggctt cctgaagtac tacaaggacc
     1501 gtatcaacgt ctcgtatgac gataaaaccg gattactgaa tattcagacg cagggcttta
     1561 gcccggagtt tgcgcttaag tttaaccaga ccgtgctgaa agagtcagag cgctttatca
     1621 atgagatgtc acatcgcatc gcgcgtgacc agcttgcctt tgcagaaacg gagatggaaa
     1681 aggcacgcca gcgtctggac gccagcaaag cggaattgct ctcttatcag gacaacaaca
     1741 acgttctgga tccacaggca caggcacagg cggcgagcac gttagtcaat acgctgatgg
     1801 gccagaagat ccagatggaa gcggatctgc gtaacttgct gacgtatctg cgtgaggacg
     1861 ccccgcaagt ggtgagtgcg cgtaatgcga ttcagtcatt acaggcacaa atcgatgaag
     1921 aaaaaagcaa aatcactgca ccacagggtg acaagctaaa ccgtatggcg gtggattttg
     1981 aagaaatcaa atcaaaagtt gagttcaaca ccgagctcta caaactgacc ctgacctcca
     2041 ttgaaaagac ccgtgtagaa gcagctcgta agctcaaggt gctgtcagtg atcagttcgc
     2101 cacagttgcc gcaggaatcg tcttttccaa atatccctta tttgatcgcc tgctggttac
     2161 tggtgtgctg cctgctgttc ggcaccctga aactgttgct ggctgtcatt gaagatcacc
     2221 gagactaacg ctgtcgctga atgagtttgt gatgaaatta tttaaatcaa ttttactgat
     2281 tgccgcctgt cacgcggcgc aggccagcgc ggccattgat attagcgctg acccaaacct
     2341 gacaggagcc gcgccgctta ccggtattct gaacggacag aagtcggata cgcaaaacat
     2401 gagcggcttc gacaataccc caccgcccgc accgccggtg gtaatgagcc gtatgtttgg
     2461 tgctcaactt ttcaacggca ccagcgcgga tagcggtgcg acggtaggat tcaaccctga
     2521 ctatattctg aatccgggtg atagcattca ggttcgcttg tggggtgcgt tcacctttga
     2581 tggtgcgtta caggttgatc ccaaaggtaa tattttcctg ccgaacgttg gtccggtgaa
     2641 agttgctggc gtcagtaata gtcagctaaa tgccctggtc acatccaaag tgaaggaagt
     2701 ataccagtcc aacgtcaacg tctacgcctc cttattacag gcgcagccag taaaagtgta
     2761 cgtgaccgga tttgtgcgta atcctggtct gtatggcggt gtgacgtctg attcgttact
     2821 caattatctg atcaaggctg gcggcgttga tccagagcgc ggaagttacg ttgatattgt
     2881 ggtcaagcgc ggtaaccgcg tgcgctccaa cgtcaacctg tacgacttcc tgctgaacgg
     2941 caaactgggg ctttcgcagt tcgccgatgg tgacaccatc atcgtcgggc cgcgtcagca
     3001 tactttcagc gttcagggcg atgtctttaa cagctacgac tttgagttcc gcgaaagcag
     3061 cattcccgta acggaagctt taagctgggc gcgccctaag cctggcgcga ctcacattac
     3121 gattatgcgt aaacaggggc tgcaaaaacg cagcgaatac tatccgatca gttctgcgcc
     3181 aggtcgtatg ttgcaaaatg gcgatacctt aatcgtgagc actgaccgct atgccggtac
     3241 cattcaggtt cgggttgaag gcgcgcactc cggtgaacat gccatggtac tgccttatgg
     3301 ttccactatg cgtgcggttc tggaaaaagt ccgcccgaac agcatgtcgc agatgaacgc
     3361 ggttcagctt tatcgcccat cagtggctca gcgtcagaaa gagatgctga atctctcgct
     3421 gcaaaaactg gaggaagcat cactttctgc ccagtcctcc accaaagaag aagccagcct
     3481 gcgaatgcag gaagcacaac tgatcagccg ctttgtggcg aaagcacgca ccgtggttcc
     3541 gaaaggtgaa gtgatcctca acgaatccaa tattgattct gttctgcttg aagatggcga
     3601 cgtcatcaat attccggaga aaacatcgct ggttatggtt catggcgaag tgctgttccc
     3661 gaacgcggtg agctggcaga agggtatgac caccgaggat tacatcgaga aatgtggtgg
     3721 cctgacgcaa aaatcgggta acgccagaat tatcgtcatt cgtcagaacg gtgctgccgt
     3781 caacgcggaa gacgtggatt cactcaagcc gggcgatgag attatggttc tgccgaaata
     3841 tgaatcgaaa aatattgaag ttacccgtgg tatttccaca atcctctatc agctggcggt
     3901 gggtgcaaaa gtgattctgt ctttgtaagg agttgagatg agcaaagcag ttattgtcat
     3961 cccggctcgc tatggctcct cgcgcctgcc gggtaagcca ctgctcgata ttgttggtaa
     4021 accgatgatc cagcatgttt acgagcgtgc gttacaggtg gcgggcgtcg cagaagtttg
     4081 ggtggcaaca gacgatcagc gtgttgaaaa ggccgtgcag gcatttggcg ggaaagccat
     4141 catgacgcgc aacgatcatg aatccggcac cgatcggctg gtcgaggtga tgcataaagt
     4201 cgaggcagat atctacatta acctgcaggg cgacgaacca atgattcgcc cgcgggatgt
     4261 agaaacgctg ttacaaggaa tgcgtgatga ccccgcgttg ccagtggcaa cgctatgcca
     4321 cgcgatttct gccgaagaag cggctgagcc aagcacggta aaagtggtgg taaataccca
     4381 ccaggatgcg ctttatttca gccgttcgcc gattccgtat ccgcgtaatg ctgaaaaagc
     4441 gcgctacctg aaacacgttg gtatttacgc ttatcgtcgc gatgtgctgc aaaactacag
     4501 ccagttaccg gagtccatgc cagagcaggc agaatcactg gagcagctgc ggttaatgag
     4561 cgcggggatc aacatccgca catttgaggt tgccgcaacc ggtccgggcg tcgacacccc
     4621 agcatgcctg gaaaaagtgc gcgccctgat ggcacaggaa ctggctgaaa acgcatgatt
     4681 ggcattttct cgtccggtat ctggcgtatt ccgcatctgg agaaatttct ggcgcaaccg
     4741 tgccagaaac tttctctgct gcgccctgtt ccgcaagaag ttgatgctat cgccgtgtgg
     4801 ggacatcgtc ccagcgcggc aaaaccggtc gccatcgcca aagcagcggg aaaacccgtc
     4861 atccgtctgg aagatggatt tgtgcgttcg ctggatctta gcgtcaatgg tgcgccgccg
     4921 ctttctctcg tggtggatga ttgtggcatt tactacgatg ccagcaagcc ttcagcactg
     4981 gagaaactgg tacaggataa agccggaaat acagctctga taagtaaggc cagagaggcg
     5041 atgcacacca tcgtgaccgg ggatttgtcg aaatataacc tggcgcctgc gtttgtggct
     5101 gatgagtcag aatgttcaga catcgttctg gttgtcgatc agacatttaa tgatatgtca
     5161 gtgacgtatg gtaatgctgg tcagaatgag ttttctgcca tgctggaggc cgcgatggcg
     5221 gaaaatcctc aagcgacaat ttgggtgaag gtgcacccag atgtactgaa aggaaagaaa
     5281 acaggttatt tcgccgatct gcgcgccacg caacgagtac gtttgattac ggagaatatc
     5341 agtccgcagt cgctgttgcg acatatttcc agagtttacg tcgtgacctc ccaatacggc
     5401 tttgaggcct tactggcagg aaaaccagtg acatgcttcg gtcaaccctg gtatgcaggc
     5461 tggggcttaa cggacgatcg tcatccgcag tcggctttgt tatctgcccg acgcggttct
     5521 gtcacgctgg aagaactttt tgccgcagca tacctgcgtt actgtcgcta tatcgaccca
     5581 ctaacggggg cagcaagtga cctgtttacc gtactgcaat ggctgcaatt actgcgcaac
     5641 catcagcaac agcgtaatgg ctatttatgg gcgccaggct taacgctgtg gaagtcagca
     5701 atcctgaaac ctttcttgcg taccgcgaca aacaggctga gttattcacg tcgctgtact
     5761 gctgccaccg cctgcgtggt atggggtgta aaaggagaac agcaatggca ggtcgaagcg
     5821 cagcgaaaat cactgccatt atggcgaatg gaagatggtt ttctgcgttc atccggactt
     5881 ggctcggacc tgctgccatc gctatcgctg gtgctggata aacgcggaat ttactatgac
     5941 gccacgcgcc ccagcgacct ggaagtgctg cttaatcaca gccagttaac gctggcgcag
     6001 cagatgcgag ctgagaagtt gcggcaacga ctggttgaaa gcaaactgag taagtacaac
     6061 ctgggagccg atttctctct gcctgccgaa gccaaaggta aaaaagttat cctggtgccg
     6121 ggtcaggtag aggacgattc ctctattaaa acaggcacta tctcaattaa gagcaacctt
     6181 gagttattac gcacagtacg cgagcgtaat ccgcacgcct acattattta taaaccgcac
     6241 ccggatgtac tggtggggaa tcgcaagggc gatattccgg cagaactgat tgctgaactt
     6301 gctgattatc aggcactgga cgcagatatt attcaatgca ttcagcacgc agatgaagtg
     6361 cataccatga cgtcgctgtc ggggtttgaa gcgttattac atggtaagca cgtacattgt
     6421 tacggcctgc ccttctatgc tggttggggt ttaaccttcg atgaacatca ctgcgggcgt
     6481 cgtgagcgag aattaacgtt agcggatttg ctatatcagg cgctgattgt ttatccaact
     6541 tatatccacc caacacggct acaacctatt actgttgaag aggcagcgga atatttgatc
     6601 cagacgtcgc gcaagccgat gtttattacc cgaaaaaaag cgggatgggt aatacgctat
     6661 taccgcaaat taattatgtt ctgcaaggtt agatttggct aaacaatttc atagttgact
     6721 attacgcaaa ttatttatgc aaggtaatgc actaaccgtt ttattatccg gtaaaaaata
     6781 tctgctattg cagggaccga tgggaccctt tttcagtgat gtagccgagt ggctggagtc
     6841 attaggccgt aacgctgtga atgttgtatt caatggaggg gattgttttt actgtcgcca
     6901 ccgacattat caggcttact accagacacc gaaagagttt cccggttggt tacgggatct
     6961 ccaccggcaa tatgactttg ataccatcct ctgctttggt gactgccgcc cattacacaa
     7021 ggaagcaaaa tattgggcaa agtcgaaagg tatccgcttt ctggcatttg aggaagggta
     7081 tttacgcccg cagtttatta ccgttgaaga aggcggagtt aacgcatatt catcgctacc
     7141 gcgcgatccg gatttttatc gtaagttacc agatatgcct gcgccgcacg ttgagaactt
     7201 aaaaccttca acgatgaaac gtataggtca tgcgatgtgg tattacctga tgggctggca
     7261 ttaccgccat gagttccctc gctaccgcca ccataaatcg ttttccccct ggtatgaagc
     7321 acgttgctgg gttcgttcat actggcgcaa gcaactttac aaggtaacac agcgtaaggt
     7381 attgccgagg ttaatgaacg agttggacca actttattat cttgccgttt tgcaggtata
     7441 taacgatagc cagattcgta accacagtaa ttataacgat gtgcgtgact atattaatga
     7501 agtcatgtac tcattttcac gtaaagcgcc gaaagaaagt tatttggtga tcaaacatca
     7561 tccgatggat cgtggtcaca gactctatcg accattaatt aagcggttga gtaaggaata
     7621 tggcttaggt gggagagtca tttatgtgca cgatctcccg atgccgggac tattacgcca
     7681 tgcaaaagcg gtggtgacaa ttaacagtac ggtgggaatc tctgcactga ttcataacaa
     7741 accacttaaa gtgatgggca atgccctcta cgacatcaag ggcttgacat atcaagggca
     7801 tttacaccag ttctggcagg ctgattttaa accagatatg aaactgtttg agaagtt
//
LOCUS       DAZKJR010000195         7826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKJR010000195 DAZKJR010000000
VERSION     DAZKJR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.196"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..270
                     /locus_tag="ACHOWS_004698"
     CDS             <1..270
                     /locus_tag="ACHOWS_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFQ0050168.1"
                     /translation="PPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALGV
                     QLHESAVVSVRHGARVRLADGRTAYWNDSTRRIDITPAQNTEAPQ"
     gene            267..671
                     /locus_tag="ACHOWS_004699"
     CDS             267..671
                     /locus_tag="ACHOWS_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050169.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLALINRKAAIARYVHSVTGRQQRK"
     gene            811..1389
                     /locus_tag="ACHOWS_004700"
     CDS             811..1389
                     /locus_tag="ACHOWS_004700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050170.1"
                     /translation="MTTATLERLNTFTDSGNVITTLMGKPYKGLAKEQCEYIQEETVN
                     NLLSLMEKNITQLNTWKDSMYGVLYELEACDEQVDSDEGLADTIASLSRSADAVTSLI
                     GFVQIAADYVEASPVLQPYSVRLVRNNRNAIRAMAGVRNALLALVDELKQRLPAKTRP
                     EPAPMTEKEAEEMINYSHKMWGLPPKNWGQEK"
     gene            1386..1817
                     /locus_tag="ACHOWS_004701"
     CDS             1386..1817
                     /locus_tag="ACHOWS_004701"
                     /inference="COORDINATES: protein motif:HMM:NF025323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafO family
                     toxin"
                     /protein_id="HFQ0050171.1"
                     /translation="MNHFVSIGSPGSDPFLDEHGLFYARQLAALLNGDAEAGYLGRVA
                     SFERNFDATSEGIKHIHIRIAGKEEPWSSKVRRFERKSDNFLIYATHWFYGNYHQVLA
                     IVSPDAHHRIDSILPDMIDYVARNFSPLTEEQLKALGDVGR"
     gene            complement(1888..2151)
                     /locus_tag="ACHOWS_004702"
     CDS             complement(1888..2151)
                     /locus_tag="ACHOWS_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFQ0050172.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(2189..3241)
                     /locus_tag="ACHOWS_004703"
     CDS             complement(2189..3241)
                     /locus_tag="ACHOWS_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0050173.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEATLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLRF
                     DVEAGHVAEEKVVPVG"
     gene            complement(3238..5043)
                     /locus_tag="ACHOWS_004704"
     CDS             complement(3238..5043)
                     /locus_tag="ACHOWS_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFQ0050174.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WAALLPAESVEDVIARRIDVLTGREGKTECELSELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGYEDGAAAGTCGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFAL
                     QFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMA
                     THDKYRTTYFSTPSAKTHQAYLVWNGDDWRGDDPARRAVEFPKESAMRVGCECPDGIW
                     RYIIRLEEAVAGGLSARVDIERIRNRYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEV
                     EMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPVHEGERFRVLAVWQWQ
                     GFNFTWQADQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNR
                     LVMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDV
                     TGHADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
     gene            5210..6259
                     /locus_tag="ACHOWS_004705"
     CDS             5210..6259
                     /locus_tag="ACHOWS_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFQ0050175.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     MLWPCHENDISYRQYTFNLGEVDALKVETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAELIPNFAKSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDISPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAATGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTAAKA
                     KLEDAIKAFSAITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGITPR
                     PAGTPAESEKPFDFV"
     gene            6271..7299
                     /locus_tag="ACHOWS_004706"
     CDS             6271..7299
                     /locus_tag="ACHOWS_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFQ0050176.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGGENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEAA
                     EEPAPGGA"
     gene            7317..>7826
                     /gene="gpM"
                     /locus_tag="ACHOWS_004707"
     CDS             7317..>7826
                     /gene="gpM"
                     /locus_tag="ACHOWS_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0050177.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIAGGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTTWR
                     LHEEIRAKWF"
BASE COUNT         1867 a         2287 c         2132 g         1540 t
ORIGIN      
        1 ccacctgaca cggaaaccgt gaaaaacggc ggcatcagtg cgcccgctgg cggcgttcgc
       61 gtgagtgtcg cactgaccgc tgagccacac atcaccgcac aaatcacccg cgagcttgat
      121 gcgctgggcg tgcagctgca tgaaagcgcc gtcgtttccg tccggcacgg tgcccgcgtg
      181 cgcctggctg acgggcgcac cgcgtactgg aatgacagca cccgccgcat tgatatcacc
      241 cccgcacaga atacggaggc gccacaatga cccgctttta ttgcgccatg cagcgacagc
      301 gttttgacga aatgatgcgc cagcgcacgc ccttactgac cctgaacaac attgccacgg
      361 ggccacaacc gggcgatgag ctggaaatca cggtcgtcag tcccgatcgc accaccacgg
      421 ccatcatcac cgacatactc aacggacgac cggacagcac taaccgcagc tggcttgtcg
      481 ccgttcgtcc gcgccgtccg cccggggagc cggagccgct cacgcctgcc ggaaaaatca
      541 tgcggcagta ccacgaagag caggcacaca aacacaacgc agaactgacc agaacactgg
      601 cattgattaa ccgcaaagcg gcaattgccc gctatgtgca ttcagtcacc ggcaggcagc
      661 agcggaaata atcgtgaaac cacaaacgcc agcggtagcc agtaagcacc agacagaacc
      721 agacagattg attaatgcgt catgtggggg taattttcgc tatcattacc actaataacg
      781 acactatggc acgttgtgag gtttacggag atgacaactg cgacactcga aagactgaac
      841 accttcactg atagcgggaa tgttatcacc actcttatgg gtaagccgta caaagggtta
      901 gcgaaagaac aatgcgagta tattcaggag gaaacggtaa ataatcttct gtcacttatg
      961 gagaaaaaca tcacgcagct aaatacatgg aaagacagca tgtatggtgt gttgtatgag
     1021 cttgaagcgt gcgatgagca ggttgattca gatgaagggc tggctgacac cattgcaagt
     1081 ctgagcagat ccgcagatgc tgttacatcc ctgattggct ttgttcagat tgcggctgac
     1141 tacgtggaag catcaccagt attacagcct tattcggtac gccttgtcag aaataacaga
     1201 aacgcaataa gagccatggc tggtgtaaga aatgctttgc ttgccctggt ggatgagctg
     1261 aagcagcgcc tgccagcgaa aacaagacca gaacccgccc cgatgacgga aaaagaggcg
     1321 gaagagatga ttaactattc tcacaaaatg tgggggttgc ctccaaaaaa ctgggggcag
     1381 gaaaagtgaa tcactttgtc agtatcggta gtccgggaag tgacccgttt cttgatgagc
     1441 atggtctgtt ttacgccagg caactggcag cgttgttgaa tggcgatgcg gaggcggggt
     1501 atctggggag agttgcatca tttgaacgaa attttgatgc caccagtgag ggcataaagc
     1561 acattcacat caggatcgcg ggaaaggaag aaccgtggag cagtaaagta aggcgtttcg
     1621 agcggaaaag tgacaatttt ttgatttatg cgacgcactg gttttacggg aattatcatc
     1681 aggtgctggc tatagtcagt cctgacgccc accacaggat tgacagcatc cttccggata
     1741 tgatcgacta tgtggccaga aacttctcgc cactgacgga agagcaatta aaggcactgg
     1801 gcgatgttgg tcggtaattt tatggcgctg gtgagtcggg gcaaaaattc tttttgcccc
     1861 ggcgaagcag caggcaagcg aagcgcgtca ggaacgccgc gacagaagtt caagcgcata
     1921 ccggcgatct tccggacgca tggcatcaac aagaaacttc accatattgc gaccggagcg
     1981 ggcgccgggg ctgagggtat gggaaaaggt catcgtacag acgaaggtgt gcccgcattc
     2041 gacatcactg catgcacagt aaagatccgc aagttcaggg gttttccagt ctgtcttccg
     2101 gatgacagca ggtgcgccac agtccggaca ccgggcttta aaaatccgca tcttacctcc
     2161 cgataacacg tatcccgggg aagacatttt acccgaccgg caccactttt tcttccgcca
     2221 catgcccggc ttccacatca aaacgaagat aaaggtgttc cggtatatcg ggatcgctgt
     2281 tcacggcgtt catgatttta cgctgaagcg gtatcacctc atccttgcgg tacgtttcac
     2341 gcgtcgtttc cgggttaggg atggtggccc cgttcgtggg gatgatcccc gcaagaccgg
     2401 caggaaagcg gtgtgccgta aacacatcct gggcgctgat ccccttgatg tttgaaaatt
     2461 catccttggc gctcacctca ccaatcggaa taattttcac cccatccggc gaacctttgg
     2521 ggatgttgat gtacagattg cggaagtttc ccagcccctt agactccgcc accttctcgc
     2581 ggatctcatc ctccatctcc gtggtgatcc ccgggtcgtt cgtatacagc aaaaaaccca
     2641 tatgtgcccc gttgttgtag taacggcgac gaaaaagggt ggcctcgctg ttcagcagcg
     2701 ccgagtgaat gccaccgata taatccggca gcccgtaaat ttgctgacgc ggatcataca
     2761 tccgcagaaa gataatatct tccggcgtat agaccagcgc ggggccttcc tgaagcacca
     2821 cgaactctcc tgttttgcgg cgccggagat acagcgacgg aagcggctca agtgccacga
     2881 cgtcgccgaa tacgttacgc accttgagca gggcgagatc gccgaacaac aggtagtcaa
     2941 ataccgcctg ttccatctgg tccgttgtga ggccaccacc ggtatagccc cccgccacca
     3001 tgttacggcg ggcatacagc accccaccgt gctgggcgtt aaggttcggg agctgcgcca
     3061 gtgccaggcg gttaatcggc agctgccagt ggttgtgttc gttgtcatac cagatgttat
     3121 gatagtccgt gccggtggtc agcaccggtt ccggctgacc aaacgtgatt gtgctgccac
     3181 gtgcgggcgt gaatgctttt ttcgtcacgc gtgacgtttt gtttctgcgt ttcttgctca
     3241 tgcggctttc ccgaaggccc atgtggacgg gcgttcatag tcataatcta caggctcatt
     3301 aatcacggcg tgcgaaatgg caaaaaacac atccgcatgc cccgtaacat ccgaccgttc
     3361 cgccacgaac gtcagcgcgt taccgctttt tgtggtggtg cggcgtatgg acatgaacga
     3421 cgcggggatc tccgtgcgtt cacggctggc ctcatcaatg gcgtctttcg cccactcgat
     3481 acgcttatgg gccaccacgt ctatcatttt catcaccagg cggtttttgc tctccacgct
     3541 gtaaagaatg gcgttagcct cacgtggcgc aaagcgactg acgatatcaa acaccccttt
     3601 cccgataccg gtgatatcta tgccgatata ggtaatgtta aagcgctgca tcagttcgcg
     3661 gatctgatcg gcctgccagg tgaagttaaa tccctgccac tgccagacgg cgagcacacg
     3721 gaaccgctcg ccttcatgga caggcggagc cacaattaca aacgtggaat tatccccgga
     3781 ccttgacggg tcaaagcccg cccacacctc acgattgccg aacggacgtg ctgccgtggg
     3841 atcgtagtca ccccacgtct ccatctccac ctcgcacctg accagttcgg agaacttaaa
     3901 taccgcatcc ttgctgtcaa caaactcgca gccgtacagc atcgcatagg tggtcgggtt
     3961 gtagcggttc ctgatacgtt caatatcaac ccgtgcagac aggccacccg ccaccgcctc
     4021 ttccagccgg ataatgtagc gccagatccc gtcagggcat tcacagccca cacgcatcgc
     4081 gctttctttc ggaaactcaa cagcacgacg cgccggatca tccccgcgcc agtcgtcacc
     4141 gttccagacc agataagcct ggtgggtttt ggcgctgggt gtcgaaaagt acgtcgtgcg
     4201 gtatttgtcg tgggtcgcca ttgccgaggc cacttcatgc agtcgcgtga attttgggat
     4261 ccagaacacc tcatcgccgt aaaggtggcc gttaaatccc tgggcggtac tggcattggt
     4321 cgacaggaaa cgcagcattg caccattgct cagacggata tgcttacccg tgagcgtgat
     4381 cccgaactgc agggcgaagt taataatata ttcacggaag atttccgcct gtacccgtga
     4441 cgcagaaaag aaaatctggt tatcaccggt caccaccgca tcttcgaacg cctcccatgc
     4501 aaaataccag gtcatgccga tctggcggct cttgaggatg aaacggcgct cacggtgccg
     4561 gttctcgcgg caatgcagct ggtaggcata aagattttta cgcgcccatt catccagcat
     4621 ttcagcagtc agtccggaaa cgtcgttttt ccggtagcgc cgctttttcc cttctccccc
     4681 gccgcaggtc cccgcagccg cgccgtcctc ataccctgcc ggacgcaggg cggcaatttc
     4741 cgccatcctt tcagcgtgtt tgtttttctg ggcgatgatc ttcacgtgct gtgcgataag
     4801 ttcacgcagt tcggaaagct cacactctgt cttgccctcc cgtccggtca gcacatcaat
     4861 acgccgggcg atcacgtcct caacggattc agcgggcagc agggcggccc actgccccac
     4921 atcacaccag tggtaaaccg tgcgcggcgg aataccgagt tccgccgcaa tatcgcgggg
     4981 gtgccaccgc ttcaggtaaa gcgatcgcgc cgcctctttg atttcgtcag aatatttagc
     5041 catgcgccca ttatggcggc gcatccggcc cgcattcagg cagaaaattt cggctgtgtt
     5101 cggctatccc gcgttatccg aagacagccg aattcagcca gtagcccccc gctgtaaaaa
     5161 tcccgacact caccggagtt cttcagattt tcacgtaaaa gaggccacga tgccgcagcc
     5221 aacgttgatg accgactgga tatgtatcgc cacatcagga ccgaccgttg acggccgcga
     5281 aattgatccg cagtggctga ctgatgccgc tgaaacctat tcccgcaaca cctacacggc
     5341 gatgctgtgg ccctgccatg agaacgacat cagttaccgc caatacacct tcaatctggg
     5401 cgaagtggac gcgctgaaag tggaaaccgg cgaggacggt aaaacccgtc tttacgcgcg
     5461 actggtccct aatcagtacc tgattgaagc caacaggctg gggcaaaaac tcttcacatc
     5521 agccgaatta ataccgaact ttgccaaaag cggacgcgac tatctcctgg gcgtggcggt
     5581 gacggatatc cccgccagtc tggggacgga aaaaatccgc ttcaccctga acaacgagga
     5641 atacacggca cagcgggcga actgccagac gttcacgctg ggcagcctgt caccggacat
     5701 ctcccctgcc ccccgtaaac ctgaatcatt cctgaaacgc ctgttcagcg caggacagac
     5761 cgacaacacc gacaccagta agcctgatac aggcgaggac gcaaaaatgg acgaaattaa
     5821 agcactgctt gaaaagctga tcaccctggt ggaaggcggc agcgccgctg ccactggcga
     5881 gggcacagca gacacgccgg aagaagcaaa agccgaagtc gcggaaatcg ccgccgaaat
     5941 cgccgacgcc gccgcagagg tggcagagct ggcagaagaa gtgaaagccg ccccggaaga
     6001 cgaggttaag gccgaagcct ttaccgctgc aaaagcgaag ctggaagacg cgattaaagc
     6061 gttcagtgcc atcacaccgg aaaaaggcga acgttaccgc cgctttgccg cccgtcgccg
     6121 ggacgacacc gacaaagaag aaatcaaagc cattgccgaa aaacttaacg acctgaccac
     6181 gaagctgtca gccaccggca tcacgccacg cccggccggc acaccggcag aaagcgaaaa
     6241 accgtttgac tttgtttaag gatacgcaac atgcaactga accagaaaac cgaatcactg
     6301 cttcgtaagt acgctgccgg gctgggtgcg gcaaacggag ttgacgacgt cacacgctat
     6361 tttgcgctgt ctgacccgaa ggagaccgcg ctgcgtgacg ccatcatgca ctccgacgag
     6421 tttttatcaa aactgccgaa cgtgctggat gttgaacagg tgacaggcca ggtggttacc
     6481 accggcgttc cgggcctcca taccggacgt aaaaaaaatg cccgcttcac ccttccgctg
     6541 ggtgttaccg ggaatgaata caagctggct gaaaccgatt ccggtgccca cctcctgtac
     6601 accacactgg tcacctgggc aaacgcaggc ggtgaaaacg agttctttaa ccgcattcag
     6661 aattttgtta acagacagat cgcgctggat atcctgcgca ttgcctttaa cggtaccagc
     6721 gtggccgaag ataccgaccc ggtaaccaac aaaaacggcg aggacgtcaa caccggctgg
     6781 catgccatcg tcaaaggccg cagcgcaaaa cagatcgtgg aggctggccc gacgctgggc
     6841 aagggtggcg actttatcgg gctggatgca gccgtggcag acctcgtgca caccctcatt
     6901 gatgagtcgt atcgcggtca tccggatctg gtggtactgg ttgcgcccga tcttgtggca
     6961 caggacaagg tgacgctgct taacgccatc gacgccccca cggaaaaagt ggccgcgcag
     7021 cttatcggtc gccagatcgc cgggctgaca gcatacaccc cgccgttcat gccggaagga
     7081 cgcctgattg tcaccacgct ggcaaacctt cacatctaca cgcagcgcgg cacccgtcag
     7141 cgccgtgcgg agtggaacga cgatcgcaag cgttttgaaa acaactatct gcgcatggaa
     7201 ggttacgccg tggagcacga cgaaatttac gcggcctacg acaagctgac catcaagggc
     7261 gcagaagcgg cagaagagcc cgcacctggc ggcgcataag cacagagggc ggcgcaatga
     7321 caccctgcca gcgacaccgg gcgaaaatcc gcacacagga ggcactcgaa cgccgggaag
     7381 cgctgacggc ctccccggtc agcttccacc tgttacgcgc ggagcttgat cgcgatgtgg
     7441 cacggctgcg cagtcttcct gtccgcgaag agcggctggc gttcaagcgc gacattctcc
     7501 tgccgcgctg gctgcccgtc gcggagcgct atatcgctgg cggaaagcgc cacgcctgcc
     7561 cggtactggt gtactgcatc atctggctgt tcgacacggg cgacctgagt cgcgcccttg
     7621 actgggcaga cattgccatc agtgaaggcc aggccacgcc ggacaacttc cgcagcaccc
     7681 tgcccgcctt cgtggccgac acggtgttgc agtgggccac cgacaacagc gaggccggac
     7741 acagcataga gccgtatttt tcgcgcacct tcaggaacgt caccaccacg tggcggctgc
     7801 atgaggaaat ccgcgcgaag tggttc
//
LOCUS       DAZKJR010000196         7786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKJR010000196 DAZKJR010000000
VERSION     DAZKJR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.197"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..297
                     /locus_tag="ACHOWS_004708"
     CDS             <1..297
                     /locus_tag="ACHOWS_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050178.1"
                     /translation="KNSKNTEFNTLKNRKEFLSSVINVNETRLQASAPAMPAIEALNA
                     FIDKGDYLKSLYVNKTRTAAEQKRLNEILRAQVISGLKASEGLAKLLIASGIML"
     gene            308..616
                     /locus_tag="ACHOWS_004709"
     CDS             308..616
                     /locus_tag="ACHOWS_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050179.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFILILTVMNNLT
                     INYGTETSPELSGISYEDANALITELILFIEDVDAENHEAYQGHLNEITRILNFAIAA
                     "
     gene            complement(668..1258)
                     /gene="cobO"
                     /locus_tag="ACHOWS_004710"
     CDS             complement(668..1258)
                     /gene="cobO"
                     /locus_tag="ACHOWS_004710"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFQ0050180.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1255..2013)
                     /locus_tag="ACHOWS_004711"
     CDS             complement(1255..2013)
                     /locus_tag="ACHOWS_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFQ0050181.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2233..3282
                     /gene="sohB"
                     /locus_tag="ACHOWS_004712"
     CDS             2233..3282
                     /gene="sohB"
                     /locus_tag="ACHOWS_004712"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFQ0050182.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3318..3569)
                     /locus_tag="ACHOWS_004713"
     CDS             complement(3318..3569)
                     /locus_tag="ACHOWS_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFQ0050183.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3949..6546
                     /gene="topA"
                     /locus_tag="ACHOWS_004714"
     CDS             3949..6546
                     /gene="topA"
                     /locus_tag="ACHOWS_004714"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0050184.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            6756..7730
                     /gene="cysB"
                     /locus_tag="ACHOWS_004715"
     CDS             6756..7730
                     /gene="cysB"
                     /locus_tag="ACHOWS_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFQ0050185.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEIMFKDIKLPEK"
BASE COUNT         2051 a         1816 c         2007 g         1912 t
ORIGIN      
        1 aaaaactcta agaatacaga gtttaacact ctgaaaaaca ggaaggagtt tctaagttca
       61 gtaatcaatg tgaatgaaac cagactacag gcatcggcac ctgccatgcc tgcgattgag
      121 gctttgaatg cctttataga taagggtgat tatctgaaaa gcttatacgt taataaaaca
      181 cgcacggcag ctgaacaaaa aagattaaat gaaatattac gcgcacaggt cattagtggg
      241 ttaaaggcgt ctgaggggct ggcaaaatta cttattgcca gcggaataat gttataggag
      301 gctttttatg aacgatgtgc aaaaccgtag aatactggca ttacttgata tgctgtataa
      361 tactttgggt aataaaacca catgtaatgt ttgtcataaa tattttatcc ttatcctcac
      421 ggtgatgaac aatctgacga ttaactacgg tactgaaact tcgccagagt taagcggaat
      481 cagttatgaa gatgccaatg cgttaattac tgaactgata ttatttattg aagatgttga
      541 cgctgagaat catgaagcat atcagggaca tttgaatgag attacacgta ttctgaattt
      601 cgctattgcc gcttgaatat aatcatgatt aaagcacctc aagaggcttc ttttgaggtg
      661 ctttcactta gtagtcaatc cctatctgcg cttttacacc ggcatcaaac gcatgtttca
      721 cggggcgtaa ttcactcacc gtatccgcca gttcaagaat atcccgatga cagcctcgac
      781 ccgtgataat caccgtctgt tgatgtggac gttcatttaa cgcctgcacc acttcttcca
      841 gtggcaaata gtcatacgcc accatatacg tcagttcatc gagcaaaacc atatccagtg
      901 aaggatcggc cagcattcgt ttggcatgtt gccagacttc acggcaggcc gcggtatcag
      961 attcgcgatt ttgtgtatcc caggtaaagc ccgttgccat cacctgaaac tcaacgccat
     1021 gtggctccag tagattgcgc tcgccattag gccaggtgcc tttaataaac tgcacgatac
     1081 ccactttttt gccatggcct actgcacgag ttgccgtacc aaatgccgcg gtggttttgc
     1141 cttttccatt gccggtaaag acgatgataa taccgcgctc atcctgggcc tgggccacac
     1201 gagcatcgac tttttctttc actcgctgct gacgctgttg gtagcgttca tcactcattg
     1261 ggaaattcct ggtttacggc ccggttgggc gtcaaaggtc atgccggttt tacggcggct
     1321 gtcatcgccc atcagccaga ggtagagcgg catgatatca gcgggtgttt taagtttctg
     1381 tggatcttcg gtcgggaagg cgctggcacg cattgcggtg cgcgtaccgc ctgggttaat
     1441 gcagttgacg cgcaggcgct gctgatattc atcggccagt acctgcatca tcccttcggt
     1501 ggcaaatttc gacgctgcat atgcgcccca gttggctcgt ccctgacgtc caacgcttga
     1561 tgaagtaaag accagtgaac cggcgtccga tttgagtaat aaaggaagca gtgcctgggt
     1621 gagcataaag gtggcattaa cgttgacctg catgacgtcc tgccagacct gcggattttg
     1681 ttcgctcatt gggcaaacat cgccgagcaa tccggcatta tgcaaaacac catccagacg
     1741 cggataatta acggcaatgc gctgtgccag ttgttggcaa tcttcggacg tgcaggtcag
     1801 caaatcgaga ataaaccact gtggctgacg tccagtttct tcgtttatgt ggctggctac
     1861 ctgacgtaat ttttcttcat tacggcccaa cagaatcact gtcgcaccat agcgtgcata
     1921 cgtcatcgcg gcttcacggc caataccatc gctggctccc gtcaccagga taatgcgatc
     1981 attgagtaaa tcttgttttg gctggtaatg catggctact cctcaacgac gttgtctgtc
     2041 gcgctgccag tgtactttat gactttgggg ctttatgcct gaaacagaag ggcatttcaa
     2101 tcagcctggg gcgataacac cgcaaaatgg atactttgtc atactttcgc tgcaataaca
     2161 tctctgcgag acggcttaac atgcctgttg taaactgtga gccaaagcgt tgtttaacca
     2221 aggtggggac tcgtggaatt gttgtctgaa tatggtttgt ttttggcgaa aatcgttacc
     2281 gttgtgctag cgattgcggc gattgccgcc attattgtca atgttgctca acgtaataaa
     2341 cgccagcgtg gcgagttacg ggtcaacaat ctcagcgaac agtataagga gatgaaagaa
     2401 gaactggccg cggcgctgat ggactcacat cagcaaaaac agtggcacaa agcgcagaag
     2461 aaaaagcaca agcaagaagc gaaagcagca aaagcgaaag ccaaactggg cgaggtggca
     2521 actgacagta aacctcgcgt ctgggtgctg gattttaaag gcagcatgga cgcccatgaa
     2581 gtgaactcgc tacgtgaaga gataacggcg gtactcgcag cattcaaacc gcaggatcag
     2641 gttgtgctcc gtctggaaag ccctggtggc atggtgcatg gttacgggtt ggcggcttcg
     2701 cagctgcagc gtctgcgtga taaaaacatt cctttaactg taacggtaga caaagtcgct
     2761 gccagcggcg gttacatgat ggcctgtgtg gcggacaaaa ttgtttccgc accgtttgct
     2821 attgtgggtt ccattggcgt ggtggcgcaa atgcccaact ttaaccgctt cctgaaaagc
     2881 aaagatattg atatcgaact gcacaccgcc gggcagtata agcgcacgct gaccttgctg
     2941 ggtgaaaaca ccgaagaagg gcgggagaaa ttccgcgaag agctgaacga aacgcatcag
     3001 ttatttaaag attttgtgaa gcgtatgcgt ccgtctctgg atattgaaca ggtggcaacg
     3061 ggtgaacact ggtacggaca acaggcggta gagaaaggcc tggttgatga aatcaacacc
     3121 agtgatgaag ttattcttag cctgatggaa ggccgtgaag tggtaaatgt acgctatatg
     3181 cagcgtaaac gactcattga ccgattcacc ggcagcgcgg cagagagcgc cgatcgattg
     3241 ttgctacgct ggtggcagcg gggtcaaaag ccattgatgt aaaagacaaa cgcgagggta
     3301 agacctcgcg ttttgcttta atcaaccagg tgatattttt ctgaaagcac atgggccagg
     3361 tgtttgaaca tattaaacac cgcggtgctt ttggctgttg gcaatccttg ttcatctaaa
     3421 aagtagtcgc cggtaaatac cagcacgcca ttacgctgcg tgacgccggt ggcttcaatc
     3481 cctgcgagcg tatcctcatg ctcacgaatg attttgttgg cctctttcaa cagcgtctcg
     3541 cggtcgatgg gttgtgtctc tttgttcatt atttactcct taaacaagga cattagtcta
     3601 cgccaggcat ggcttgcaga caaatatacc acgctggtgg caagagcgcc ttactggcaa
     3661 ctttggattt tgcatgctaa taaagttgcg tatcggattt tatcaggtac agtgtgacgc
     3721 tttcgtcaat ctggcaatag atttgcttga cattcgacca aaattccgtc gtgctatagc
     3781 gcctgtaggc caagacctgt taactcagtc acctgaattt tcgtgaacag agtcacgaca
     3841 aggggttgat atccgcagag agcgagtcca tatcggtaac tcgttgccag tggaaggttt
     3901 atcaacgtgc gacgcattcc tggaagaatc aaattaggta aggtgaatat gggtaaagct
     3961 cttgtcatcg ttgagtcccc ggcaaaagcc aaaacgatca acaagtatct gggtagtgac
     4021 tacgtggtga aatccagcgt cggtcacatc cgcgatttgc cgaccagtgg ctcagctgcc
     4081 aaaaagagtg ccgactctac ctccaccaag acggctaaaa agcctaaaaa ggatgaacgt
     4141 ggcgctctcg tcaaccgtat gggggttgac ccgtggcaca attgggaggc gcactatgaa
     4201 gtgttgcctg gtaaagagaa ggtcgtctct gaactgaaac aactggctga aaaagccgac
     4261 cacatctatc tcgcaaccga ccttgaccgc gaaggggaag ccattgcatg gcacctgcgg
     4321 gaagtgattg gtggtgatga tgcgcgctat agccgagtgg tgtttaacga aattactaaa
     4381 aacgcgatcc gccaggcatt taacaaaccg ggtgagctga atattgatcg tgttaatgcc
     4441 cagcaggcgc gtcgctttat ggaccgcgtg gtggggtata tggtttcgcc gcttctatgg
     4501 aaaaagatcg ctcgtggtct gtctgccggt cgtgtgcagt cggtggcggt tcgcctggtg
     4561 gtcgagcgtg agcgtgaaat taaagcgttc gtaccggaag agttctggga agtcgatgcc
     4621 agcacgacca cgccatctgg tgaagcgttg gcgttgcagg tgactcatca gaacgacaaa
     4681 ccgttccgtc cggtcaacaa agaacaaact caggctgcgg taagtctgct ggaaaaagcg
     4741 cgctacagcg tgctggaacg tgaagacaaa ccgacaacca gtaaacctgg cgctcctttt
     4801 attacctcta cgctgcaaca agctgccagc acccgtcttg gatttggcgt gaaaaaaacc
     4861 atgatgatgg cgcagcgttt gtatgaagca ggctatatca cttacatgcg taccgactcc
     4921 actaacctga gtcaggacgc ggtaaatatg gttcgcggtt atatcagcga taattttggt
     4981 aagaaatatc tgccggaaag tccgaatcag tacgccagca aagaaaactc acaggaagcg
     5041 cacgaagcga ttcgtccttc tgacgtcaat gtgatggcgg aatcgttgaa ggatatggaa
     5101 gcagatgcgc aaaaattgta ccagttaatc tggcgtcagt tcgttgcctg ccagatgacc
     5161 ccagcgaaat atgactccac gacgctgacc gttggtgcgg gcgatttccg cctgaaagca
     5221 cgcggtcgta ttttgcgctt tgatggctgg acaaaagtga tgcctgcact gcgtaaaggc
     5281 gatgaagatc gtatcttacc ggcagtcgat aaaggcgatg ctctgacgct cgttgaactg
     5341 acaccagccc agcactttac caaaccgcca gcccgtttca gtgaagcatc gctggttaaa
     5401 gaactggaaa aacgcggtat cggtcgtccg tctacctatg cgtcgatcat ttcgaccatt
     5461 caggatcgtg gctatgtgcg tgtagaaaac cgtcgtttct atgcggaaaa aatgggcgaa
     5521 atcgtcaccg atcgccttga agaaaatttc cgcgagttaa tgaactacga ctttaccgcg
     5581 cagatggaaa acagccttga ccaggtggca aatcacgaag cagagtggaa agctgtactg
     5641 gatcacttct tctcggattt cacccagcag ttagataaag ctgaaaaaga tccggaagag
     5701 ggggggatgc gcccgaacca gatggttctg accagcatcg actgcccgac ctgtggtcgc
     5761 aaaatgggga ttcgcacagc gagcaccggg gtattccttg gctgttccgg ctacgctctg
     5821 ccaccgaaag agcgttgcaa aaccaccatc aacctggtgc cggaaaacga agtgctgaac
     5881 gtgctggaag gcgaagatgc tgaaaccaac gcgctgcgcg caaaacgtcg ttgcccgaaa
     5941 tgcggcacgg cgatggacag ctatctcatc gatccgaaac gtaagttgca tgtctgtggt
     6001 aataacccaa cctgcgacgg ttacgagatc gaagagggcg aattccgcat taaaggttat
     6061 gacggcccga tcgttgagtg tgaaaaatgt ggctctgaaa tgcacctgaa aatggggcgt
     6121 ttcggtaaat acatggcctg cactaacgaa gagtgtaaaa acacgcgtaa gattttacgt
     6181 aacggcgaag tggcaccacc gaaagaagat ccggtgccat taccggagct gccgtgcgaa
     6241 aaatcagatg cttatttcgt gctgcgtgac ggtgctgccg gtgtgttcct ggcggccaat
     6301 accttcccga aatcgcgtga aacacgtgcg ccactggtgg aagagcttta tcgcttccgc
     6361 gaccgtctgc cggaaaaact gcgttatctg gccgatgcgc cacagcagga tccggaaggt
     6421 aataagacca tggttcgctt tagccgtaaa accaaacagc aatatgtctc ttcggaaaaa
     6481 gacggaaagg cgactggctg gtcagcattt tatgttgatg gcaaatgggt tgaagggaaa
     6541 aaataacctt taattctgtc aggtttttat aaacaaaggg tcgcgaaagc ggcccttttt
     6601 tattgcatat tattttttct tcacacctat acactaaggc tataaatgat atagtggtta
     6661 tagttagcac tttttttatt attaaatcgt attagtcacc cgccaggtgt gacgaaaaaa
     6721 cgatgttctg atggcgtcta agtggatggt ttaacatgaa attacaacaa cttcgctata
     6781 ttgttgaggt ggtcaatcat aacctgaatg tctcatcaac agcggaagga ctttacacat
     6841 cacaacccgg gatcagtaaa caagtcagaa tgctggaaga cgagctaggc attcaaattt
     6901 tttcccgaag cggcaagcac ctgacgcagg taacgccagc agggcaagaa ataattcgta
     6961 tcgctcgcga agtcctgtcg aaagttgatg ccataaaatc ggtcgccgga gagcacacct
     7021 ggccggataa aggctcgctg tatatcgcca ccacgcatac ccaggcacgc tacgcgttac
     7081 caaacgtcat caaaggcttt attgagcgtt atccccgcgt ttctttgcat atgcaccagg
     7141 gctcgccgac acaaattgct gatgccgtct ctaaaggcaa tgctgatttc gctatcgcca
     7201 cagaagcgtt gcatctgtat gaagatctag tgatgttacc gtgctaccac tggaatcggg
     7261 ctattgtagt cactccggat cacccgctgg caggcaaaaa agccattacc attgaagaac
     7321 tggcgcaata tccgttggtg acatatacct tcggctttac cggacgttca gagctggata
     7381 ctgcctttaa ccgcgcaggg ttaacgccgc gtatcgtctt cacggcaacg gatgctgacg
     7441 tcattaaaac ttacgtccgg ttagggctgg gggtaggggt cattgccagc atggcggtgg
     7501 atccggtcgc cgatcccgac ctggtgcgcg ttgatgctca cgatatcttc agccacagta
     7561 caaccaaaat tggttttcgc cgtagtactt ttttgcgcag ttatatgtat gatttcattc
     7621 agcgttttgc accgcattta acgcgtgatg tcgttgatgc ggctgtcgca ttacgctcta
     7681 atgaagaaat tgagatcatg tttaaagata taaaactgcc ggaaaaataa tttcttgcgt
     7741 tattttcggc accttttatg tagcgaaggt gccggaatat attctc
//
LOCUS       DAZKJR010000197         7355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKJR010000197 DAZKJR010000000
VERSION     DAZKJR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.198"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..400)
                     /locus_tag="ACHOWS_004716"
     CDS             complement(<1..400)
                     /locus_tag="ACHOWS_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFQ0050186.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANML"
     gene            complement(413..2194)
                     /gene="gcl"
                     /locus_tag="ACHOWS_004717"
     CDS             complement(413..2194)
                     /gene="gcl"
                     /locus_tag="ACHOWS_004717"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFQ0050187.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMSVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTEACFMHYE"
     gene            complement(2284..3099)
                     /gene="allR"
                     /locus_tag="ACHOWS_004718"
     CDS             complement(2284..3099)
                     /gene="allR"
                     /locus_tag="ACHOWS_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFQ0050188.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3177..3659)
                     /gene="allA"
                     /locus_tag="ACHOWS_004719"
     CDS             complement(3177..3659)
                     /gene="allA"
                     /locus_tag="ACHOWS_004719"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFQ0050189.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3889..4815
                     /gene="allS"
                     /locus_tag="ACHOWS_004720"
     CDS             3889..4815
                     /gene="allS"
                     /locus_tag="ACHOWS_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFQ0050190.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            4884..5978
                     /gene="mnmH"
                     /locus_tag="ACHOWS_004721"
     CDS             4884..5978
                     /gene="mnmH"
                     /locus_tag="ACHOWS_004721"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFQ0050191.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVKGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTR"
     gene            6094..6459
                     /gene="ylbG"
                     /locus_tag="ACHOWS_004722"
                     /pseudo
     CDS             6094..6459
                     /gene="ylbG"
                     /locus_tag="ACHOWS_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7226..7355)
                     /locus_tag="ACHOWS_004723"
                     /pseudo
     CDS             complement(7226..7355)
                     /locus_tag="ACHOWS_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1880 a         1909 c         1755 g         1811 t
ORIGIN      
        1 tcagcatatt cgcagcataa cgcaggtttt ctacaagcgt tgtgtgaatc tgttcactgc
       61 tgaaaccagt cggcgtttta ccgaccagac agttaatttt tttattaccc agagcacgag
      121 cataacgaat cgctgctgct acgccatccc gaaactcttc ttcacggcca ggaatacagg
      181 caataccccg ctcgcccgcc gcccagtcac ctgccggtaa attgtgcagc gtatgttcga
      241 gtttattact cgccagcacc tgttttaatt cttcaatgtc gtagtcataa gggaacataa
      301 attcaacgcc gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga aaatcatatt
      361 caccaaataa catcgataaa ttagcagaga aacgtaacat tatttatctc ccttattcat
      421 agtgcatgaa acaggcttca gtcggtgcat ccgctgcgtt atcggcgata tcttcaaatt
      481 ccatgacgtt atccagttca ctgcccatcg aaatattggt cacacgctcg agaataactt
      541 ccacgactac cggtacccga tattgcgcca ttaagatttt cgcctgttca aaggctggtg
      601 caatatcttc cggtttgaaa acgcgaattg ctttacaacc taaaccttcc gctactttta
      661 cgtggtcgac gccataaccg ttcacttcgc tggagttaat attttcgaac gcgagctgta
      721 cgcagtaatc catatcaaag gcacgttgcg actggcgaat cagacccaga taggcgttat
      781 tgaccagtac atggatgtac ggaatgttga actgtgcgcc aacggctaat tcttcaatca
      841 ggaactggaa gtcgaagtca ccggaaatcg ccaccacatt gcgctctgga tcagcggcac
      901 aaacccccag cgccgccgga atcgtccagc ccagcgggcc tgcctgccca cagttgatcc
      961 agtggcggtc tttaaagaca tgcagcattt gtgccgcagc gatttgtgac aggccaatgg
     1021 tggtgacata acaaacatcg cggccaaagg ctttgttcat ctcttcatac acgcgctgcg
     1081 gtttcaccgg cacgttgtcg aagtgggttt tgcgcagcaa agtgcgtttg cgctgctggc
     1141 agtcggcgac ccattcttta cgacacggca gacgacccgc tttttgcatc tcctgcgcca
     1201 cttcaaccag cagcgtcagc gccgctttag catcagagac aatgccgaga tccggacaca
     1261 gcacgcggcc aatttgcgtc ggctcaatat cgatatgaac gattttgcgc ccttcggtgt
     1321 atttctctac cgaaccggta tgacggttag caaaacggtt accgatacca aacaccatgt
     1381 cggacgccag cagcgttgcg ttaccgtaac gatgcgcggt ttgcagaccc accatcccgg
     1441 ccatcagttc atgatcgtcg ggaatacagc cccagcccat cagcgttggg atcaccggaa
     1501 tgctggtcag ttcagcaaac tgttgtaaca gtgcagctgc gtcagcattg attaccccgc
     1561 ccccggcaac aatcaccgga cgttcggcct ggattaacat ttctaccgct ttttcgatct
     1621 gcatacggct ggcagcaggt ttgtagaccg gcagcggttc gtacatgtca ggatcaaact
     1681 cgatttccgc aacctgaacg tcgaacggta aatccaccag taccggaccc ggacgaccag
     1741 aacgcatcag gtgaaatgcc tgttgcagca cgcgaggcac cagcgccgct tcacgtactg
     1801 taaccgacat tttgctgacc ggtttagcaa ttgcttcaat atcaacggcc tggaaatctt
     1861 ctttatgcag acgggcgcgc ggagcctggc cggtaataca cagaatagga atggaatcag
     1921 cagaagcaga atagagcgcg gtgatcatgt cagtgcctgc tggtcccgag gttcccagac
     1981 atacgccgat attccctgcc gttgcgcggg tataaccttc cgccatgtgc gaagcacctt
     2041 ccacatgacg cgccagaatg tgacgaatac cgccgtgctt acgcatcgct gaatagaaag
     2101 gattgattgc tgctcccgga acgccgaagg cggtggtgat accttctttc tccagcacat
     2161 acattgccgc gtcaacggct ctcatttttg ccattttatt cctacctcta ttaattggaa
     2221 aaattttcca actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg gatgcgacag
     2281 acactatgga tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg tgtctctgac
     2341 cagctcaccc tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc cggagatgga
     2401 gatagcggca acaacactac ctacatcatc gtaaattgct gaagctatgc aattcaggcc
     2461 gacaacatgc tcttctttat ctacggtata gcccagttca cgcgcttgtt ccaggtcctt
     2521 cagcaaggtg ggcatatcca caagcgtagt tggcgtaaac tgctgcaaac cggtttgcag
     2581 aatgatgctc atcaactcct cttccgccag cggataaagc agcgccttgc ccgcaccgga
     2641 agcatgcagc ggcagacgac tgcccagcgg cgcacacatc ctgaccatcg atttacactc
     2701 taactgacca attaataccg cttcattgcc gttacggatc gcgacattga ccgtttcgcc
     2761 ggaaagtaac atcaggcggc gcataaacgg cccggcgacg gagagtacat cgcggttatg
     2821 gatgtacgct gcaccgacgt taaagacacc taatcctata tgccaccagc ctaactgact
     2881 gtcctgatag acaaaatccg ctgcctgtaa aaccttcagc aagcgaaagg tcgtggagag
     2941 cggcaaatcc agattgagag aaatatcgct aaccgacgaa cttcccccac ttttttccag
     3001 atattgcaga atcgcaattc cccgctctaa cgcctgtgcg cccttctgtg ccacaggctc
     3061 cgcctgtcct ggcctgccgc gccgtctaac ttccgtcata cctacgttaa ctccgaccta
     3121 caagtgagca taaacaatga acaattcgtt tatcttatgc aagtcggttg caggcgtcac
     3181 gcaaaacaga gttcctgttc aggaatactt tcaacatcac agttgtcact gccgccgcga
     3241 tcgatggtca gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg ccagacgtta
     3301 cgatggtaat tcaccccctg ttcaccgttg gtaataaacg cccgcagcgt tgacaggtct
     3361 ggtttgtcgt cacctaacgc cacgactacc acaaacactt cacctttcat cgggataaac
     3421 gcctgtgtac ccaacggatg acgttcgagt tcgtgaatag tcagcggcag attcgccggt
     3481 tgcgcgcggt taatgctgat aagcgtacgg tcttgctcaa gaatctcaac cagcgccaaa
     3541 tcgtggtaac gctctaccag gccattgtta atatggaaaa aatcccgttg ctgcgtttcg
     3601 attacgtcgc cataagcact aaaggcttcc tgacttaacg gtaatacctg aagtttcatc
     3661 tttttgtcct tgtaacataa ttccgttcaa ccaccgccga ccggtcagct tggtttcaat
     3721 aacaggcaat ctaaaacctt aagagaacaa catcaataca aaagttggaa tatttttcca
     3781 aaactagatt tagtccacaa attgaacagg gttatcatgt aaaaatactg ataaatcaga
     3841 ggataagaaa atcacgcaac ttgcggattt aaaaggggat acctgaagat gttcgatcca
     3901 gaaaccttgc ggactttcat tgcggttgct gaaacaggaa gtttttcaaa agcggcagaa
     3961 cgattatgta aaaccacggc gacgatcagt tatcgcatta aacttctgga agagaatacc
     4021 ggagtagcgc tgtttttccg tacgactcgt agcgtgacgt tgacggcggc tggcgagcat
     4081 ctactttgtc aggccagaga ctggctgagc tggctggaaa gtatgcccag cgagctgcaa
     4141 caggtgaatg atggcgtgga acgccaggtg aatattgtca tcaacaacct gctctataac
     4201 ccccaggccg tcgcccagtt gctggcgtgg ctgaatgaac gttacccctt tacccagttt
     4261 cacatctccc gacaaatcta tatgggcgtc tgggattcgc tattatacga aggtttttca
     4321 ctggctatcg gcgtcacggg aaccgaggcg ctggcaaata cctttagtct tgatccctta
     4381 ggatcggtgc aatggcgctt tgtcatggcg gcggatcatc cgctggcgaa cgttgaagag
     4441 ccgctaacag aagcacagtt gcggcgcttt ccggcggtta atattgaaga cagcgcccgc
     4501 acactaacca aacgcgtcgc ctggcgattg ccggggcaaa aagagattat tgttcccgat
     4561 atggaaacga aaatcgccgc ccatctggcg ggcgttggca ttggtttttt gccaaaatca
     4621 ctttgccagt caatgatcga taatcaacaa ctggtcagcc gggtaatccc aacgatgcgc
     4681 cctccttcac cattgagtct ggcatggcgc aaatttggca gcggcaaagc gatagaagat
     4741 attgtgacct tgtttaccca gcgcaggccg gaaatcagcg gatttttaga aattttcggc
     4801 aacccacgca gttaaaaatc aagtttcctt gcgcgttggc gcgtaaaata gcgcgcaagc
     4861 tgatttgagg ttcacttttt actatgcaag agagacacac ggaacaggac tatcgtgccc
     4921 tgctgattgc tgatacgccc attattgatg ttcgcgcccc catcgagttt gagcaaggcg
     4981 caatgcctgc cgctatcaac ctgccgttaa tgaataacga tgaacgcgcc gccgttggca
     5041 cctgctataa acagcaaggg tcagacgcgg cgctggcgct gggtcataaa ctggtggcgg
     5101 gtgaaattcg tcagcagcgt atggacgcct ggcgggcagc gtgcctgcaa aatccgcaag
     5161 gtattctctg ctgcgcccgt ggcggtcagc gctcacatat tgtgcaaagc tggttgcatg
     5221 cagcggggat tgattatccg ctggtgaaag gcggttataa ggcactgcgc cagaccgcga
     5281 ttcaggcgac tattgaactg gcacaaaaac cgatagtgct gattggcggt tgtaccggca
     5341 gcggtaaaac gctgttagtg cagcaacagc cgaacggtgt tgatctggaa gggttggcgc
     5401 gtcatcgcgg ttcggcgttt ggtcgcacct tacaaccaca acttagccag gcgagttttg
     5461 aaaacctgct ggctgccgaa atgctaaaaa ccgacgcccg tcagaatttg cgcctgtggg
     5521 tgctggaaga tgaaagccgg atgatcggtt cgaatcacct gccggaatgc ctgcgcgagc
     5581 gaatgaccca ggcggcgatt gcggtagtag aagatccgtt tgagatccgt cttgagcggc
     5641 tgaacgaaga atatttcctg cgtatgcatc atgattttac ccacgcgtac ggcgacgaac
     5701 agggctggca ggagtattgc gaatacctgc atcacggact ttcggcgatt aagcgtcggc
     5761 tggggctaca gcgctataac gaactggctg caaggctgga tgcggcgctg acaacgcaac
     5821 ttaccaccgg cagcaccgac ggtcatctgg cctggctggt gccgttactt gaagaatatt
     5881 acgacccgat gtatcgctat cagcttgaga aaaaagcgga aaaagttgtc tttcgcggtg
     5941 agtgggcgga agtggcggaa tgggttaaga cgcgataatg agggacgtat ttgtattagt
     6001 cgcgacttga tctgacacat ggccttgaaa ggttgagagt taccggtttt gatatgagtg
     6061 tcgaatcctt atccaaaaca agaggtaact ctcatgcttc atactgccaa tcccgtcatc
     6121 aaacacaaag ccggtttgct caatctggct gaagaactca gcaacgtatc aaaagcctgt
     6181 aaaatcatgg gcgtttcgcg tgatacgttt tatcgttacc gcgaactggc cgctgaaggc
     6241 ggtgtggatg cgcagattaa tcgtagtcgc cgcgctccta accttaagaa ccgtaccgat
     6301 gaggcaattg aacaggctgt tgttgattac gccgtcgctt tcccgacaca tggtcagcac
     6361 cgggccagca acgagctgcg taaacagggg gtttttatct ccgacagtgg tgtccgttct
     6421 gtctggttac tccataacct tgagaatctc aaaagacgat actgaacaaa ttttatcagg
     6481 tgacgttccg taaaaagttg tatggagttc tcgatgcagt cggcgcgact atcagtaccc
     6541 ccgctgaacc ctggactatc gggccgccgt actggggcat gtcaccctgg cgtgccgctg
     6601 gttttccgct aatggtttat ttcctctttc tttcccggcg agaatttatt ttaatccgga
     6661 aggattattt attttcagat agggactcac attaaacacg taacatttta attaatgcat
     6721 ggctgaatat gtattacaca atgtcagata tcgtaaaaga ccagtgcaat tctatgtaaa
     6781 ctctctggct gttcatttta tttgttgttt cagggtcgga actcaccctc aataaacaac
     6841 tgaatacgag tagatattca tcgtctgagc tatatggctt tacacaatag ccgacatgtc
     6901 ttttctcagt cagggattat acttttcctc actacgaagt tgacgacttc gatatgggat
     6961 agactcttaa ttcaagcaat tactctgcac aagcatcagg taacccagct ccaatatttg
     7021 ttataaaata atgcgtaaca ttaatttcac caacaaaaaa aaggtttacg ctatcccctt
     7081 gccctggtat tttaccatat ttacaccaca ctttttttgt atctgtatca ttctctgata
     7141 cgattttcgc cacttgttcc ctggccctca cagtcaatgt tttcttaaac ctccccccat
     7201 catcgattgt tagggaaaaa taagaaaaaa aataatcatc ttctacattt gtggatattg
     7261 atatatccac cgctacgtgc ggaaaaaaat ctgggagcca tgtaaaaacc ccatcacttt
     7321 ctggtattgc catatagttt atgtaatgct tactt
//
LOCUS       DAZKJR010000198         7325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKJR010000198 DAZKJR010000000
VERSION     DAZKJR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.199"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..333)
                     /locus_tag="ACHOWS_004724"
     CDS             complement(34..333)
                     /locus_tag="ACHOWS_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0050192.1"
                     /translation="MTYEVIITSEAEQQIINLHRYITEKAGSAIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGVYHGGQSYESDFL"
     gene            complement(339..596)
                     /locus_tag="ACHOWS_004725"
     CDS             complement(339..596)
                     /locus_tag="ACHOWS_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFQ0050193.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            732..1010
                     /locus_tag="ACHOWS_004726"
     CDS             732..1010
                     /locus_tag="ACHOWS_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050194.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWLQHPPCTEEQAEW
                     LIQCYRRRGYEFEKALSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1012..2061
                     /locus_tag="ACHOWS_004727"
     CDS             1012..2061
                     /locus_tag="ACHOWS_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFQ0050195.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAAWMIDVIRHAMNDTQERE
                     LSLAELCWWAVCHQVADALPESVLRRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            2074..2448
                     /locus_tag="ACHOWS_004728"
     CDS             2074..2448
                     /locus_tag="ACHOWS_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFQ0050196.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKSGERYRRAVALIVRQQ
                     QLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            2445..3266
                     /locus_tag="ACHOWS_004729"
     CDS             2445..3266
                     /locus_tag="ACHOWS_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFQ0050197.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            3494..3691
                     /locus_tag="ACHOWS_004730"
     CDS             3494..3691
                     /locus_tag="ACHOWS_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFQ0050198.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            3842..4894
                     /locus_tag="ACHOWS_004731"
     CDS             3842..4894
                     /locus_tag="ACHOWS_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFQ0050199.1"
                     /translation="MLSTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELES
                     ERFLQTVKEIRDDFCR"
     gene            4983..5705
                     /locus_tag="ACHOWS_004732"
     CDS             4983..5705
                     /locus_tag="ACHOWS_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0050200.1"
                     /translation="MAVVCSVILVCSPINIFIEKDKLSLVPGMVILVTRNIRELFRTY
                     TDRVKVADINHDIVSQYLEGGRRRMSSPQACFPHYLTSGIHQPVLAEALITNHGFPSA
                     PSGDLSTMTGLSCLSLFANDESLSSFLSRCLSNTSDKVRAIIQSDISVNWKLGVIALQ
                     LHMSESLLKKKLKDEGNSFSRLLLEERMRGAVNMLCFQYGRGAIVAEKCGYSSLSYFI
                     SVFHRYYGVPPDKYVSLHMVDF"
     gene            6783..>7325
                     /locus_tag="ACHOWS_004733"
     CDS             6783..>7325
                     /locus_tag="ACHOWS_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFQ0050201.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGI"
BASE COUNT         1836 a         1691 c         2020 g         1778 t
ORIGIN      
        1 gttatttaag ttttttgtta gtgaaaatat aaactataaa aagtcagatt cataactttg
       61 ccctccatgg taaacgccag cgataaagac tttattgcca tcaacggcaa aagcaatgat
      121 cgttctgtgg cggaagtgag ttacccgcat tccctgtcga atatcatcgc gtttattgcc
      181 ccgatgcggg aatgtggaaa aaccatcaag ataatcaaga agcgcgttgg cataattgtc
      241 agcaatggcg ctccctgcct tctctgttat atatctgtgc aggttgatta tctgctgttc
      301 ggcctcagaa gtaatgatga cttcatacgt catgcagatt acttcccggc tcgaatcgcg
      361 gcgcgaacct gtgaaatgga gcgtccgttg tttgggtttt cgcggataga atcgagagat
      421 ggggcggctg aatgcgttaa ccacgcttcg attgctttat cgcgctcatt cagtgcgcga
      481 agcccttcac gaatgacctc actttctgaa gcataggcac cggaagccac acgggcgcgc
      541 accatgtcag ccatctcgtt agttaatgta atgctgaatt gttgggttgt acgcatggta
      601 aacctcacgg agtaggatag aacactattc gatgatagca cactgcctgt tgacgacacc
      661 agaaatcaga tacaatattg ccgcacgcca gcctgaacaa ctggcacctg ctgcgccagc
      721 agagacaacc gatggcgcac gataccagat tttacaattc ggataactcc gccgcccctg
      781 ccagcaggca cgggcggcgt tctcatgcat tcaaatctga ctggttacag catcccccat
      841 gcactgaaga acaggccgaa tggctgatcc agtgttaccg caggcgcgga tatgagtttg
      901 agaaagccct cagcttcgat cgtcgtcact ggataatctc cgtcaggctc ccttattccg
      961 aacgcccacc gcgtccgtcc cgcacattcc agcagcgcat ctggaggtaa cgtgcgggta
     1021 ttacttcgac ctgttctggt accggaactc gggatggtgc tccttaagcc gggccgtgaa
     1081 tcaatgtcag cattccataa cggcagaata ttggtggaac cggaaccgaa aaacatgcgc
     1141 ggtctgccgt ccggagtcgt tcctgccgtt cgacagccgc tggcagagga taaaacatta
     1201 ctgccatttt tcagcaacga acgggtgatt cgtgctgcgg gtggcgctgg tgcactgtct
     1261 gactggttat tacgtcacgt gaaatcctgc cagtggccac acggtgatta tcatcacagc
     1321 gaaaccgtca ttcaccgtta cggtaccggc gcgatggtgt tgtgctggca ctgtgacaac
     1381 cagctgcgcg accagacatc agaatcactc gatcaacttg ctcagcagaa tctggcagcc
     1441 tggatgattg atgtcatccg tcacgcaatg aatgacacac aggagcggga actgtcgctg
     1501 gcagagttat gctggtgggc tgtttgtcat caggtggctg atgcgcttcc ggagtctgta
     1561 ttgcgtcgtt cgctgggatt accagcggaa aaaatccgct ccgtataccg tgagagtgac
     1621 atcgtaccgg gagaacagac agccatcagc atactgaagc agcgcacaaa aaatattgcg
     1681 ctgccacttc acgtccacca gcaacaaaat ccaccacaga aaaaaacggt tgtcagtatc
     1741 gctgttgatc cggagtctcc tgaatcgttc atgaggcggc ctaaacgtcg ccgttgggtt
     1801 aatgagaaat acacgcgctg ggtaaagaca cagccgtgtg cgtgttgtgg taagcctgct
     1861 gacgatccgc atcacctgat tggtcatggt caggggggaa tggggacaaa ggcccacgat
     1921 attttcacgc taccgttgtg ccgggagcac cacaacgaac ttcatgcaga cccgctgaag
     1981 tttgagaaaa agtacggctc tcagattgag ttaatttttc gttttcttga tcacgccttt
     2041 gcgactggcg tgctcgggta aaagaggtga ctgatgctca tagatttggt tttaccttac
     2101 ccgccgacgg tgaacactta ctggcgacgc cgtggcagca catattttgt atcaaaatcc
     2161 ggggagcgtt atcgccgggc agtggcactt attgttcgcc agcagcagtt gaaattaagc
     2221 ctgtccggac ggctggcaat aaaaattatt gcagagccac cggataagcg tcgtcgtgac
     2281 ctggacaata ttctgaaagc accgctggat gcgctgacgc acgcggggtt gttaatggac
     2341 gatgagcagt ttgatgaaat caatattgta cgtggccagc cagtatctgg tggacgtctg
     2401 ggggtgaaga tttaccccat aatgcttgaa gggcaggtca aaaaatgaaa ctggaagatt
     2461 taccgaaata ctactcccca aaatcccccg gcctgactga tgcatcggcc tcaacgtcga
     2521 aagatgcgct gagtatcact gatgtgatgg ccgcgcaggg catgacacag aatcgggctg
     2581 agatggggtt ttctgcgttc cttgggaaaa tgggcattag tatgaatgac agagagcggg
     2641 caacagaatt gctgacagaa tatgcactca gtcggtgtga tcgcgtggcg gcgttaagaa
     2701 aactcccggc agaaataaaa ccggcagtga tgcgtattat ggcttcgtat gcgtttgaag
     2761 attatgcccg tagcgcggcg agcaaaaaac agtgcccctg ctgtcacgga aaaaaattta
     2821 ttgaaagcga ggtttttaca aacaagatcc agtatccgga tggtaagccg ccagtgtggg
     2881 caaagtgcac aaaaggcgtg tatccgtctt actgggagga atggaaaaaa gtcagggagg
     2941 tggtaaaagt tgcctgtccg gagtgtggag ggaagggaga ggtatccact gcctgtaaag
     3001 attgtcgtgg gcgcggtgtt gccattcatc gtgaagagtc ggaaaaacag ggtgtgccgg
     3061 ttttcagaaa ctgtcagcgt tgtggtgggc gtggctatga aagattacct tcaacggagg
     3121 catttaatgc catatgtaat gtaaccgatg ccatatctct cgatacatgg aaaaaaacag
     3181 ttaaacgttt ttacgatacg ctggtggtgc agtttgatat tgaagaagca tgggcagaac
     3241 aacaactgaa aaaggtgacc agatagcttt gttgattttt cccgaatctg tggtaaattt
     3301 gccctaacga tgggtgtttt atgcctgacg ttagaagatt ttttacaccc cgccgcctgg
     3361 cgggtttttt tatgactgaa atcacgccag tacagtaaac gcgctggtgg cggtgaatac
     3421 cggtctttca gcttgctggc tttttcgaca agagttattg gtgtgtcacg ttaaccggaa
     3481 aagggaaaaa gacatgctga aacagcagga tatgaccgaa accgccagag tggtgtttaa
     3541 tgaattaagc gtcaccgaac cggcgactgt cggggagatt gcacagaata cttacctttc
     3601 acgtgaacgc tgccagttaa tactgaccca gctggttatg gcggggctgg cagactatca
     3661 gttcggttgt tacagacgcc ttcagtcctg aaggcttttt tatttgtggt aaatgggcgg
     3721 ctggtgggtg ttaggggcac tcaccagcca tctgctcatg cgtccggatc acaagcaaac
     3781 ctcaggccca ctgctttgcg caaaagcaga atgagcctat cagagacagg cttaatgatc
     3841 catgcttagt actgtaaaaa tatccagttg tgagttaatc aacgccgact gcctggaatt
     3901 tatccggtcg ttacccgaaa attctgttga cctgatagtc acggacccgc cgtactttaa
     3961 agtgaagcct gagggctggg ataaccagtg gaagggcgac gatgattacc tgaagtggct
     4021 ggaccagtgt cttgcgcagt tctggcgggt gctgaaacct gccggaagtc tttacctgtt
     4081 ctgtggccat cgcctggcat ctgacattga aatcatgatg cgtgaacgct tcagtgtgct
     4141 gaaccatatt atctgggcga agccgtccgg acgctggaac gggtgcaaca aggaaagcct
     4201 gagggcgtat ttccccgcca cagagcgcat tctgttcgcg gaacattatc aggggccgta
     4261 tcgtccgaaa gatgccgggt atgaggcgaa gggcagggca ctgaaacagc atgtgatggc
     4321 cccgctgatt gcttactttc gtgatgcacg tgctgccctg gggataacgg caaaacagat
     4381 tgctgatgcc acaggaaaga aaaacatggt gtcgcactgg ttcagtgcca gtcagtggca
     4441 gctaccggac gaaagcgatt atctgaaatt acaggcgctg tttgcccggg tggcagaaga
     4501 gaagcatcag cgcggagaac tggaaaagcc acatcaccaa ctggtcagca catacagtga
     4561 actgaaccga cagtatacgg aactgcagag tgaatataag catctgcggc ggtatttcgg
     4621 tgtgacggcg caggtgccgt acaccgatgt gtggacgcat aaaccggtgc agtactatcc
     4681 cgggaaacat ccgtgcgaaa aaccggcaga aatgctgcag cagataatca gcgcgagcag
     4741 tcgtccgggt gacttggttg cagatttctt catggggtcg ggctcaaccg tcaaagcagc
     4801 gctggcactg gggcgtcgtg caactggtgt tgagctggag agtgaacgtt ttttacagac
     4861 agtgaaggag atccgggacg atttttgcag gtgacagagg ttgaggaaag gttgaaggga
     4921 ggatcatttg ttattgttgt atataattat tctacccctt tcctgagtca ggtaaatata
     4981 taatggcagt tgtctgttct gtaattctgg tttgttcccc gatcaatatt tttattgaga
     5041 aagacaaact ttcacttgtg ccgggcatgg tcattctggt caccagaaac atcagggagc
     5101 tttttcgcac ttacacagac cgggttaagg ttgctgatat aaatcacgat atagtcagtc
     5161 aatatctgga aggtgggcga aggcggatga gctccccaca ggcatgtttt ccgcattatc
     5221 tcacttctgg cattcatcag cctgtactgg cagaggcgtt aattactaat catggatttc
     5281 ccagtgctcc ttctggtgat ttgtccacga tgacagggct ttcatgtctt tctctgtttg
     5341 ccaacgatga gagcctgtca tcatttttgt caaggtgttt aagcaataca agcgataaag
     5401 tcagggcaat aattcagagc gatatatccg taaactggaa gcttggtgtt attgcgttac
     5461 aactgcatat gagcgaaagc ttgttaaaga aaaagcttaa agatgaagga aacagcttca
     5521 gccgcctgtt gctggaagag aggatgcggg gggcagtaaa tatgttatgc tttcaatatg
     5581 gacggggagc gatagtggca gaaaaatgcg gttattcaag cctgtcttac tttatatcag
     5641 tattccacag gtattatgga gtacctcctg acaaatatgt gtctctgcat atggttgact
     5701 tttaatttca tcctgctggc ggatggagtg ctggtggtcg gcattgtggt ggtgcttttt
     5761 cctttggtta aaaaggacat gccaggtagt tagcgggtat cagtcatgcc cagaaaattt
     5821 taaatgtctc acaattcaga cggttgacag ttgtctgttt tgcggggagt ttgttaaaag
     5881 aaactggcat ggtgaatccc cctgagcgga ggggcgactg gtgcatcagg tgttatcttt
     5941 gatacccagg aaagtgaacg cgcgggttcg gtggcaccgg gctgaactca ccgggaggca
     6001 cccggcacta tgcgcatgat gtacagatac gcggcttcag cccctctccg gaggggcttt
     6061 tctgtgcctt atgcatcaca gtttctggaa ccctgagtac tacagtatca gtcagggtgc
     6121 tatatttgca gatgtgatga aagcctgccg gcaggcaggg cgtatcggaa atgacccagt
     6181 aagagaaaac gttgactcag ataccggtgc tgagttaccg ggaaaccggc atcacatgac
     6241 cgctatcctt catggctctc tcaggagagc ctttttactg gagaaaaccg cttccccgta
     6301 aaatgccgtg ttgctcacaa ttcagtcagt tgaaggttgt ctgcccgact gagaatttgt
     6361 taaaaaattt ctgcatgatg aatccccctg tgcggagggg cggctggtgt acggaatggt
     6421 ctctgatgat cgttagcgag aatgacgcgg gttcggtggc accgggctga actcaccggg
     6481 aggcacccgg catcatgtaa tgaacggtac atagcgctac tcttcagccc ctctccggag
     6541 gggctttctt atggataaaa aaagcccgca tggtgagatg cgggcagcaa ggaataaaca
     6601 acaaaacgtg aagtaatcaa tttttcagct ggcgaataat acccgacagt aatcactctg
     6661 cgcaactgcg cggccttttt cgtattgcgg gctgtagtct tcctcctgcc attgtcctgt
     6721 aacttccgga cttcagcccg cccctcatct gactcacaac attatcccga ccgggaggat
     6781 tcatggcatt taaacactat gacgtggtca gggcggcgtc gccgtcagac cttgcggagc
     6841 gactgactca aaaactgaag gaggggtggc agccatttgg cagtcctgtc gccatcacgc
     6901 cttataccct gatgcaggcc attgcggcgg aaggtgatgt caccacacct gtggtggtga
     6961 agccgtcgga tggagaaggc acagttatca gcgccaccag cgacccggag tattactttg
     7021 ttgtggttct ggcagggcag tcaaacggca tgtcgtatgg tgaaggcctt ccgctgccgg
     7081 agacatatga ccgtccggac ccgcgtatta agcagctggc gcgccgaagt acggtgacac
     7141 cgggaggtgc cgcctgtaag tataacgaca ttattccggc ggaccattgt ctgcatgatg
     7201 tgcaggacat gagccgtctt aaccatccga aagcggacct gtcaaagggg cagtacggaa
     7261 ccgtggggca ggggctgcat atcgccaaaa aactgctgcc gtttataccg gcgaatgcgg
     7321 gcatt
//
LOCUS       DAZKJR010000199         7302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKJR010000199 DAZKJR010000000
VERSION     DAZKJR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.200"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..481
                     /gene="curA"
                     /locus_tag="ACHOWS_004734"
     CDS             <1..481
                     /gene="curA"
                     /locus_tag="ACHOWS_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFQ0050202.1"
                     /translation="CRHATEVLGFDVCLDHHADDFAEQLAKACPKGIDIYYENVGGKV
                     FDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDY
                     GHRIQEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNFGKVVIRVAVDD"
     gene            679..1344
                     /gene="mcbR"
                     /locus_tag="ACHOWS_004735"
     CDS             679..1344
                     /gene="mcbR"
                     /locus_tag="ACHOWS_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFQ0050203.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(1380..3482)
                     /gene="pqqU"
                     /locus_tag="ACHOWS_004736"
     CDS             complement(1380..3482)
                     /gene="pqqU"
                     /locus_tag="ACHOWS_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFQ0050204.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVISELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMSPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            3724..4785
                     /locus_tag="ACHOWS_004737"
     CDS             3724..4785
                     /locus_tag="ACHOWS_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFQ0050205.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(4898..6397)
                     /gene="ansP"
                     /locus_tag="ACHOWS_004738"
     CDS             complement(4898..6397)
                     /gene="ansP"
                     /locus_tag="ACHOWS_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFQ0050206.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            6664..7281
                     /locus_tag="ACHOWS_004739"
     CDS             6664..7281
                     /locus_tag="ACHOWS_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFQ0050207.1"
                     /translation="MIKVHGVPGWGTTISEMMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISSIAEAVCQLPKLQEVLKRNEII"
BASE COUNT         1972 a         1802 c         1779 g         1749 t
ORIGIN      
        1 atgccgccat gctactgagg tgctgggctt cgatgtttgt cttgaccatc acgcggatga
       61 ttttgccgaa caactggcga aagcgtgccc aaaaggtatt gatatctatt atgaaaacgt
      121 gggcggtaag gtatttgatg cagtgctacc tttacttaat acatctgcgc gcattcccgt
      181 ttgtggctta gtgagcagct ataacgctac agagctacca cccggtccgg atcgtttacc
      241 tctgctgatg gctacggtgc tgaaaaaacg tattcgcttg caaggattta ttatcgctca
      301 ggattatggt caccgcatcc aagagtttca gaaggagatg gggcaatggg tgaaagagga
      361 taaaatccac taccgcgaag aaattactga cggtttagag aatgcgccac agacgtttat
      421 cggcctgctg aagggtaaaa acttcggcaa agtggtgatc cgcgtggcgg ttgatgatta
      481 aagtttcaac ggcggcgtaa gccgccataa aaatccaggc aaaaagctta ttttcagctt
      541 taattaacta accacgtcat tgttctgctg ctaataacaa caaaggtgag tggtttatga
      601 ttattcactt taatacacca ggtgaactcc ttctgccatg caggcagggt tggacagaaa
      661 acacgcgaag gaattaaaat gcctggaacg gaaaaaatga aacatgtcag tttgactctg
      721 caggttgaga acgacctgaa acatcagctt agtattggcg cattaaaacc tggcgcacgc
      781 ctgattacta aaaatctggc ggagcaatta ggtatgagta ttacacctgt gcgtgaagca
      841 ttattacgtc tggtttcggt gaatgcgctt tctgtcgcac cagcgcaagc atttacagtt
      901 ccggaagtgg ggaaacgtca attagacgaa atcaatcgga tccgctacga gctggaatta
      961 atggcagtta ctctggctgt tgaaaacctc accccgcaag acctggcgga actcgaggaa
     1021 ttgctcgaga aattacagca ggcgcaagaa aagggcgaca tggaacaaat cattaatgcg
     1081 aacaggctat ttcgcttagc gatttatcat cgttcaaata tgcccatcct gtgtgagatg
     1141 attgagcaac tgtgggtcag gatgggacct ggtttacatt atctctatga agcgattaat
     1201 ccagcggaat tacgggagcg tatagaaaac tatcgtctat tactcgccgc gttaaaagca
     1261 aaagacaaag agggatgcag acattgtctt gctgaaatta tgcaacaaaa tattgctatt
     1321 ttataccagc aatacaatcg ttaaattaaa tgcccggcaa ataccgggca tcaaggtaat
     1381 tactcaaatc gccacgcaat attcatgccg acaccataat ttcgcccggg cgcaggttcg
     1441 taatatcgcc cgtttgactc attgacaatg acagagccaa cgtattcttt atcgaataaa
     1501 ttatcgacac gaccaaataa atccacagtt aaattgtggt aattgtattt atacccggtg
     1561 aataagccga cgagagtata agacggcgct ttggccgtat tttcatcatc tgccataata
     1621 tcgcccatat aacgcgcttc cgtacctgca taccagccat cttccggtac ataacctatc
     1681 gacgcaaagc ccatattacg ggcgatccct ggcatccggt taccgttaca atcctgttca
     1741 ttgcaaacat tgctgcgata ggtcgcatca agccaggtcc aggacgcttt tacgcgaaaa
     1801 tctcccgcga aacgttgatc ccatgccagt tcagcgcctt gacgacgggt ctttccggca
     1861 tttttgtaag tcgtacgccc accgctactg ctatcgacaa caatttcatc atcggtgtcg
     1921 gtctggaaca atgcgagact aagcagccca tcaccaatac gcgttttact gccgatctca
     1981 attgtatcgt tggtggaggg ttttaaacca aagttcatac cgctttgccc atcagcgcga
     2041 taagacagct cattaatcgt cggcgtttca aaaccgcgcc cggctgccag atagacattc
     2101 caggcatcgg tcattgcata ttttaacgat ccggctggta gccatttgtg ataactggca
     2161 tcaccgctgt cgtcgccgtt acccggagta acgtaatgat cattggaatc aaaccacaca
     2221 gagctgtagc gcacgccagc atccagcgaa agtttgtcgg tcagttgcca ctgggtttgt
     2281 aaatagggat cgacgttcca catcaaattg cgttcgtcgc ggcgcaactc acctttttgc
     2341 ccgtattccg gcacgccgcg attcaggcgg aagttattgt aacccttgcg gttttcactc
     2401 atgttttcgt agttcaggcc ggttgtgaac gtgactggaa cgcccaattc accacggtgt
     2461 gtccagcggc tgtctatccc ctggtaatgg cgttgcaggg taatcacgcc gcccgcatgt
     2521 gacgggttaa gttgcgggga catggggatt gactggtact gggtcgtttc tcgctctccg
     2581 gcatacatca ttacactcat atcatcttgc gcgctcaggc tacgttcata gcgcaaccca
     2641 gcctgagttt gcttgatggt ttttcgcgtg tcgtactgtt ctgcacgggg cgcttgttgt
     2701 ggattcgcct tccattctgc ttcggttagc ccacctgggt catctgcttt gatatccaca
     2761 ctattgaaaa tcaggcttaa tttgctggcg tcatcaatgc gaacgcccag tttggcattg
     2821 gctaaatttt tctgtgcgcc actatgatca cgatagccgt gggtcgtaaa gcgcgtggtt
     2881 gagacggtgt aatcgacatc gcctggctgt gtgccgtctc ccgttgcgcc cgttgctttc
     2941 agcccatagc gccagctgcc aaaactgccg tagtaactac tggcttcaat ggttggtggc
     3001 tgttgtccgg tctgggtgat gacattcatt acaccgccag acgcgttgcc atacagagca
     3061 gagaaggggc cacgcagcac ttccacattt tgcacactgc ttaaatcgat attggatgtc
     3121 tgcccttgtc cgtcgggcat ggtagcggga ataccgtcaa catacaggcg aataccgcga
     3181 attccgtaag tggagcggga gccaaatccg cgaatcgaca gctgtaaatc ttgcgcatag
     3241 ttctgccggt tttgtacctg caaaccaggc acgccagtca gtgattcaga caagttaatg
     3301 cgcggtgttg ccaggcgcat ctcctcgcca tccaccacgc tcacggctgc tggggtatcc
     3361 agttctgaaa tcacctgcgg cgcggcactg acaatcatgg tctgttcatc agcggcaaaa
     3421 acggtggggg aaaggacaag cagcgcggga aaaacggtct gtcggacgga aaaaatcttc
     3481 atgaaaaaag ccaggttaag aatgggaaaa caccgtcatg gtaataaaat tgtaaattta
     3541 tggaaaacga aacggcacga tatgttaagt aattgagaaa attgtagtcg taacggcaag
     3601 aaatgctcca catttgagaa aataatgatt accattccca tttataacaa gagcgtaacg
     3661 atgattacgc ttagcggagc attgtgaagc agcaaaaata tcggttcatc aaagggagtc
     3721 gtcatgcatt tacgtcatct gttttcatcg cgcctgcgtg gttcattact gttaggttca
     3781 ttgcttgttg cttcatcatt cagtacgcag gccgcagaag aaatgctgcg taaagcggta
     3841 ggtaaaggtg cctacgaaat ggcttatagc cagcaagaaa acgcgctgtg gctcgccact
     3901 tcgcaaagcc gcaaactgga taaaggcggc gtggtttatc gtcttgatcc ggttactctg
     3961 gaagtgacgc aggcgatcca taacgatctc aagccgtttg gtgctaccat caataacacg
     4021 actcagacgt tgtggtttgg taacactgta aacagcgcgg tcacggcgat agatgccaaa
     4081 acgggcgaag tgaaaggccg tctggtgctg gatgatcgta agcgcactga agaggtgcgc
     4141 ccgctgcaac cgcgtgagct ggtggcagac gatgccacga acaccgttta catcagtggt
     4201 attggtaaag agagcgtgat ttgggtcgtt gatggcgaga atatcaaact gaaaaccgcc
     4261 atccagaaca ccggtaaaat gagtaccggt ttagcgctgg atagcaaagg caaacgtctt
     4321 tacaccacta acgctgacgg cgaattgatt accatcgaca ccgccgacaa taaaatcctc
     4381 agccgtaaaa agctgctgga tgacggcaaa gagcacttct ttatcaacat cagccttgat
     4441 accgcaaggc agcgtgcatt tatcaccgac tctaaagcgg cagaagtgtt agtggtcgat
     4501 acccgtaatg gcaatattct ggcgaaggtt gcggcaccgg aatcactggc tgtgctgttt
     4561 aaccccgcgc gtaatgaagc ctacgtgacg catcgtcagg caggtaaagt cagtgtgatt
     4621 gacgcgaaaa gctataaagt ggtgaaaacg ttcgatacgc cgactcatcc gaacagcctg
     4681 gcactgtctg ccgatggcaa aacgctgtat gtcagtgtga aacaaaaatc cactaaacag
     4741 caggaagcta cccagccgga cgatgtgatt cgtattgcgc tgtaatagat atggcgaggg
     4801 tgaaaatgga gagacttgcc tgacttttat cacgccgtat ccggcacctt cgtcagtaat
     4861 ttgaatttgc ccggcacgta gccgggcaat tttgcaatta gctcgccgtt tcaggcttaa
     4921 acacaatttc ctgtttttct tcatcttcct cgacgactgg cgcagtgctg tgaatttcag
     4981 caacgcgttt gcgcacacca aaccagccta taaccagcag aataccgata atcggcagcg
     5041 ccgcgatagt ataagtcccg ttcgggtaat cgaacgccat cagcacaagg acgctcagca
     5101 aaaacagtaa tgtcagccag gaagtgaagg gcgcgccagg cagtttaaaa ctgacatccg
     5161 ctgctttgcc ttctttaatc gctttacgca ggcgcatctg gcacacgatg ataaacgccc
     5221 atgaagcgat gattcccagc gacgcgaagt tcaacacaat ctcaaatacg cgcgacggca
     5281 ccagatagtt gaggaatacg ccgacgacat acacaaccag tgtcgccaga atcccggcat
     5341 acggcacatg ctgacgactc attttcgcca taaaactcgg tgcggaaccg cccatcgcca
     5401 tcgagcgcag aatacgtccg gtgcagtaca gacctgagtt caggctggag agggcagcgg
     5461 tcagcaccac aatgttcata atgctgccga tgtacggcac acccagttta gagaaaaacg
     5521 tcacgaacgg actttgcccc gcctgatacg cgctccacgg caataacata accagcaaca
     5581 ccacggagcc gacgtaaaac aggccaatac gccaaatcac actgttaatc gctttaggca
     5641 ccatggtctg cggatcttta cattctccgg cagctgtacc caccatttca atggaggcaa
     5701 aagcaaacac tacgccctga atcaacacca gcgcaggtag caaaccgtgg gggaagaagc
     5761 cgccgttatc ggtaattaaa tgaaagccag tggtgttgcc atccagcggc tgaccactac
     5821 cgaggaacac tgtacccacg accaaaaagg tcacaatggc gagcacttta ataagcgcaa
     5881 accagaactc catctcggca aaccatttca caccaatcat attcatggtg ccaacgatgg
     5941 tcagcgcagc gagcgcaaag acccactgcg gtacaccacc aaatgcgccc cagtaatgca
     6001 tatacagtgc aacggcggta atatcgacaa tccccgtcat cgcccagttg atgaagtaca
     6061 tccagcctgc aacataagcg gctttctcac ccaaaaactc acgggcataa gagacaaaac
     6121 tgccactgga agggcggtgt agcaccagct cacccaatgc acgcagaata aaaaacgaaa
     6181 acaagccgca gattaaatag accagtgcca gtgctggccc cgccatttgc agtcgggctc
     6241 ccgcgcctaa aaacaaaccc gtaccaatcg cgccgccaat ggcgatcatc tgcacctggc
     6301 gattgcccat cgctttgtga tacccctctt cgtgggcatt aagccagcgg cgtttcgcgg
     6361 cgtgctgatc tgaagtgtcg gtgtcgtgtt tactcattgc tctccctgat tgctttaatg
     6421 aaaaagtcat ataagttgcc atgaacaatg tttattcatc ctggcgatga tagttatcac
     6481 cattattctt tatgggtata aaactatcgg cgcagcatcc tacccgttat cgataaacga
     6541 tgcaaaacat ccccttacaa ttctgaaggg gattcataca actgacgaaa aaatgacaaa
     6601 tacttttgct ggttaacctg tgtgctgtcc tagacttaat ctttaaaaga ttgtgagggg
     6661 catatgatta aggtgcatgg cgtacccgga tggggcacta caatcagtga gatgatgcta
     6721 acactggctg atattcctta tcagttcgtt gatgtcagtg gttttgacca cgagggaact
     6781 tcacgcgagt tattgaaaac cctgaacccg ttgtgtcaga ttccgacact ggcgctggaa
     6841 aatgacgaga ttatgacgga gacagcggcg attgcattga tggttctcga tcgtcgcccg
     6901 gaccttgcgc caccggttgg gcgcgccgaa cgtcagcagt ttcaacggct attggtctgg
     6961 ctggttgcta atgtctatcc aacattcact ttcgccgatt accctgagcg ttgggctcct
     7021 gacgcacccg aacagttaaa gaataatgtt attgaatatc gtaaatcgct ttatatctgg
     7081 ctgaattcac aattgaccgc tgaaccttat gcgtttggtg agcaactaac gctggtggac
     7141 tgttatcttt gcaccatgcg cacatggggg ccggggcatg aatggtttca cgataatgcg
     7201 cagaatatca gctcgatcgc tgaagctgta tgtcagttac cgaaattaca agaagtatta
     7261 aaaaggaatg aaattatttg aatgggatac cagaagatta tg
//
LOCUS       DAZKJR010000200         7299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKJR010000200 DAZKJR010000000
VERSION     DAZKJR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.201"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            242..2038
                     /locus_tag="ACHOWS_004740"
     CDS             242..2038
                     /locus_tag="ACHOWS_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFQ0050208.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            2056..2724
                     /locus_tag="ACHOWS_004741"
     CDS             2056..2724
                     /locus_tag="ACHOWS_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFQ0050209.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSLA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2739..3410
                     /gene="dsbI"
                     /locus_tag="ACHOWS_004742"
     CDS             2739..3410
                     /gene="dsbI"
                     /locus_tag="ACHOWS_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFQ0050210.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIVRG"
     gene            complement(3456..4244)
                     /gene="ygiD"
                     /locus_tag="ACHOWS_004743"
     CDS             complement(3456..4244)
                     /gene="ygiD"
                     /locus_tag="ACHOWS_004743"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFQ0050211.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4387..5160
                     /gene="zupT"
                     /locus_tag="ACHOWS_004744"
     CDS             4387..5160
                     /gene="zupT"
                     /locus_tag="ACHOWS_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFQ0050212.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(5218..5388)
                     /gene="yqiD"
                     /locus_tag="ACHOWS_004745"
     CDS             complement(5218..5388)
                     /gene="yqiD"
                     /locus_tag="ACHOWS_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFQ0050213.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(5650..6303)
                     /gene="ribB"
                     /locus_tag="ACHOWS_004746"
     CDS             complement(5650..6303)
                     /gene="ribB"
                     /locus_tag="ACHOWS_004746"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFQ0050214.1"
                     /translation="MNQTLLSSFGTPFERVENALVALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(6407..6555)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6677..6976
                     /gene="ubiK"
                     /locus_tag="ACHOWS_004747"
     CDS             6677..6976
                     /gene="ubiK"
                     /locus_tag="ACHOWS_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFQ0050215.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(7011..7211)
                     /gene="glgS"
                     /locus_tag="ACHOWS_004748"
     CDS             complement(7011..7211)
                     /gene="glgS"
                     /locus_tag="ACHOWS_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFQ0050216.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT         1948 a         1810 c         1711 g         1830 t
ORIGIN      
        1 acaatgttgc agggatcaca ctacacagta ttgaacattg tgattgatat attgcccccc
       61 tactatccat taaacataaa tatttgtttc accgaataac aacatattta aaagaaacac
      121 aaaagcattc tcactcttta ttgaattaat atttatattc acgtaacgaa tttattagca
      181 ccctctcaac agtcgatatt catcattatt atatatttgc gttatataag gagatatatc
      241 catgtttgat aaatatagaa aaacactcgt agccggaact gtggcgataa ccctgggttt
      301 gtcagcatcg ggggtgatgg ctgcgggttt taaaccagcg ccgcctgccg ggcaactggg
      361 tgcggtcatt gtcgatccct acggcaatgc accactgacc gctttggttg acttagatag
      421 ccatgttatt tctgacgtca aagttaccgt ccatgggaag ggcgaaaaag gtgtagaaat
      481 cagctatccc gtgggtcagg aatcactaaa aacttacgat ggtgtaccga tttttggtct
      541 ttatcagaaa tttgctaaca aagtgaccgt tgagtggaaa gaaaacggca aggtcatgaa
      601 agatgattat gtggtgcaca cttcggccat cgtcaataat tacatggata accgctctat
      661 atccgattta caacagacca aagttattaa agtcgcaccg ggttttgaag atcgcctcta
      721 tctggttaat acccacacct ttaccgccca aggttccgat ctccactggc atggtgagaa
      781 agataaaaat gccggtatcc ttgatgcggg tccggcaacc ggcgcactac cttttgatat
      841 cgcgcccttt actttcatcg tcgataccga aggtgaatac cgctggtggc tggatcaaga
      901 cactttctac gatggtcgtg accgcgatat caacaagcgt ggttatctga tgggtatccg
      961 cgaaacgcca cgcggcacct ttaccgctgt acaaggtcag cactggtacg agttcgacat
     1021 gatggggcag gtgctcgaag atcacaaact accgcgcgga tttgctgacg ccactcatga
     1081 gtccattgag acgccaaatg gcacggtact gttgcgcgta ggtaagagta actatcgtcg
     1141 cgatgacggc gtacacgtca ccaccattcg tgaccatatc ctcgaagttg ataaatctgg
     1201 tcgcgttgtc gatgtatggg atctgaccaa gatcctcgat ccgaaacgcg atgcactgct
     1261 cggcgcgctg gatgcaggtg cagtttgcgt taacgttgac cttgcccatg caggacaaca
     1321 ggcaaaactg gaaccagata caccgttcgg cgacgcgctg ggtgtagggc caggccgcaa
     1381 ctgggcgcac gtgaactcta tcgcctatga cgcaaaagat gactctatca tcctctcctc
     1441 tcgccaccag ggtgttgtga aaattggtcg tgataagcaa gtgaaatgga tccttgcgcc
     1501 ctctaaaggt tgggaaaaac cgctggccag caagctgctg aaaccggttg atgctaacgg
     1561 taagccaatt acctgtaacg aaaatggcct gtgcgaaaac tcagacttcg actttaccta
     1621 cacccagcat accgcctgga tttccagcaa aggaacgctc accatttttg ataatggcga
     1681 tgggcgtcat ctggaacaac ctgccttacc aaccatgaaa tactcccgct ttgtggaata
     1741 taagattgat gagaagaaag gcaccgttca gcaagtgtgg gaatacggta aagaacgtgg
     1801 ctacgatttc tatagcccga ttacctccat cattgaatat caagccgacc gtaacaccat
     1861 gtttggcttc ggtggttcta ttcatttgtt cgatgtcggg cagccaaccg tcggtaagtt
     1921 gaacgaaatc gattacaaaa ccaaagaagt gaaagtggaa atcgacgtgc tgtcagataa
     1981 acccaatcag actcactatc gtgcactgtt agtccgtcca caacagatgt tcaaataatt
     2041 aagaagggaa taaaaatgtc gaaattaggg atctcatcac tatttaaaac catactgctt
     2101 accgcagctt tggctgtttc atttaccgct tctgcattta ccgaaggcac ggattacatg
     2161 gttctggaaa aacctattcc gaatgccgac aaaacgctga ttaaggtatt cagctacgcc
     2221 tgcccgttct gttacaagta cgacaaagcg gttaccggtc cggtatcgga aaaagtgaaa
     2281 gatatcgttg ctttcacgcc gttccatctg gaaaccaaag gtgaatacgg caaacaagca
     2341 agcgaagtct ttgcggtctt gatcaataaa gataaagcag caggcatttc cttatttgat
     2401 gctaactctc agtttaagaa agccaagttt gcctactacg cggcttatca cgacaaaaaa
     2461 gaacgctggt ctgacggtaa agatccggct gcttttatca aaaccggtct ggatgccgca
     2521 ggtatgagct tggcggattt tgaagctgca ctgaaagaac cagcggttca agaaacactg
     2581 gaaaaatgga aagcctctta tgatgtcgcc aaaatccagg gggttccagc ctatgtcgtt
     2641 aatggtaagt acttgatcta caccaagagc atcaaatcca tcgacgcgat ggcagacctt
     2701 atccgtgagt tagcaagcaa ataacaggga aacaggctat gggtattaag ggaatgtgga
     2761 aagaccttcg cacatcacca gttgacacac tggtcagatg gcaggagcaa cgcttgttgt
     2821 ggttgttaat ggccgttgcg atgggcgcgt taattatcct ggcgcactct ttcttccaga
     2881 tctatctcta catggcaccc tgtgagcaat gtgtatacat tcgctacgcc atgtttgtga
     2941 tggttattgg aggattggtt gcagcgatca acccaaagaa catcatccta aagctaattg
     3001 gctgcgtgat ggcattttac ggcagcattt tgggactgaa gttttcgctc aaactaaacg
     3061 atatccacca tgcagtacat aatccggatc ccgattcact gtttggcgta cagggttgct
     3121 ctaccgatcc aacttttccc tttaacctgc cgctggcaca gtgggcacca aattggttca
     3181 agcctacagg ggattgtggc tatgacgcac caatcgttcc tgatggcgtc acactgagca
     3241 gcacgcaaca gtggtttgtg gaaatgtatc agcaatctga aggctggtat ctgctaccac
     3301 catggcattt tatgaatatg gctcaggctt gtatgctggc ttttggtatg tgtctggtgc
     3361 tgctggtcat tatgagtggc gcatgggcgc ttaaaatcgt cagaggctaa ctcagcaaca
     3421 cccgaagcca gtcgttctgg cttcgggttc ttttattagc ctatctgcac cgacaacata
     3481 cttaaactgc ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac
     3541 gcacctaaga catacaacaa cggcagatag tgctctggcg ttgggttcga taacgcgcca
     3601 ccttcatggt cgagataatt caccagagga tgctgctcta ccggcccttg ccacgtcaga
     3661 ttcgctttca catactcatt aaacgacgtc gcccacggat acggtgaact atcaccgtgc
     3721 cacttcactg tgcgcaggtt atgcaccacg ttaccgctgg cgaccaacat tattccttca
     3781 tctcgcagcg ccgcgagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg
     3841 tcgatactca actgcaccat cgggatatcc gcgtcaggat acatcttaat cagcacgccc
     3901 caggaaccgt ggtcaaagcc ccaggcttct ttatccagcg tcaccgggat cggcgctaac
     3961 agctcaacca gacgctgtgc cagcgcaggc gaacccggag caggataatg cgtatcgtac
     4021 agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact
     4081 cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat
     4141 gtcatcccca acgtctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga
     4201 ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact
     4261 aaaggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa
     4321 caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag
     4381 ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc
     4441 gcgtttctcg gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt ttcgctagga
     4501 tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagcc
     4561 gccgaaggaa tgtcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctat
     4621 tttggcctgg accgcatgtt gccacatgcg catccgcagg atttaatgca aaaatcggtg
     4681 cagccgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg
     4741 cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctgggc
     4801 tttggcatcg cactggccgt tgcgttacac aatattcctg aaggtctggc ggttgcaggc
     4861 ccggtttacg cggcaacggg gtctaaacgc accgcaattc tttgggcggg gatttctgga
     4921 ctggcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg
     4981 gtggtcatgg cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat
     5041 gaattaatgc cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta
     5101 tgtggaatgt cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa
     5161 aagcaacatc agaagttgtc cagcgcatgg acaacttctg ccagcttcac taaagcctta
     5221 ttctaccttt tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag
     5281 cagtgcgtct ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc
     5341 caccacaaca agagcaatca atacaatcca gtaccaggcg ataaacatcc ttttaccctt
     5401 aatatttaac aatactaaac atttagcgca taaatttcac atatcctttt tcggatatat
     5461 tcatatggtc gcataataac ggaacaatta tatgattttg atcataatga aaaagaatgc
     5521 ctgatatcaa tgaaattttc tcatagaaag agaagcggaa ttaacatctt gcatagcagg
     5581 gcaaaataaa aaccgaagcc agctacaact tcggtttctt gattaagaca gtaaattaag
     5641 cagcgatttt cagctggctt tgcgttcatg cgcctgacga taagccacca ggtcttcaat
     5701 agtcacgagc gccatattgt gtttattggc aaactcaatg cactctggtg cacgcgccat
     5761 cgtgccatcg tcattagtca gctcacacag cacaccagcc ggtttaaagc cagccagcgt
     5821 catcagatca atagtcgctt cagtatggcc gccacgcgtc agtacaccac cagcctgagc
     5881 gcgaagtggg aaaacgtggc ccgggcgatt cagatcagaa ggttttgcgc catcggcaat
     5941 cgctgcgcga acggtcgtaa tacggtcagc ggcagaaaca ccggtagtca caccttcagc
     6001 tgcttcaatg gtcacggtaa aaccggtgcc ataggcgctg gtgttatttt ctaccatcat
     6061 tggcagatcg agttgtttac ggcgatcttc agtgatgcac aggcaaacaa taccgctacc
     6121 gtggcgaatg gtcagcgcca tctgctcaac ggtcatggtt tctgccggga agatcatatc
     6181 accttcgttt tcacggtctt catcatcaag caccattaca ccgcgtcctt cacgcagcgc
     6241 aaccagtgca ttttcaacac gttcgaaagg cgtaccaaaa gaggaaagta gcgtctgatt
     6301 catggtaaaa aaacctcact aaaattatgg ttaccagaat cagggcagtc ttaggagtgg
     6361 cggcatatag ccaaaataac gtgagcgggt ccatgcccga cagaatcgtt actctctccc
     6421 atccggactc taaccgtcgg ccccggaatt acaccggatc tgctgtcctt tgagttcgca
     6481 cccaaagcgc tcgcgggctt tcaactgagt tgatttaccg ccggtgggga atttcgcccc
     6541 gccctgagaa taagcggatt cactataacg ctaatgatta gcggcagcaa cgcatagctt
     6601 cacataattc tggtttatga cttaccctta tcgcactaca atggcactca acacctatca
     6661 gtacagggaa actacaatga ttgacccgaa aaaaattgag caaatcgctc gccaggttca
     6721 cgaatcaatg cctaaaggaa tcagggagtt cggggaagat gtggagaaaa aaatccgcca
     6781 aaccctacaa gcgcagctga cgcgtctcga tctggtaagc cgcgaagaat tcgacgttca
     6841 gacgcaagtc ctgttacgga ctcgtgaaaa actggcgtta ctggagcagc gcatcagtga
     6901 actggaagcg cgcaataatt cggtagcaga tcttcaatct cctccggcta tcccgcctat
     6961 tgataaagca gaataaagca tcacaaaaac gggccagacg gcccgtaata tcagtgttct
     7021 aattcaagtt ctctggtttg catcatctgt tggcaatacc aggcataacg tgcgcaaaac
     7081 caggttctat gttcttcatc catgttacca gtaacggcca gaatatcgac cgggcgacct
     7141 tctgcgtgca ttctggcaaa actacgcgcc aggaaatcga aattatttaa agaattaaga
     7201 ctatgatcca taagcacatc ctccggttat cgcatacctt ttaaggccat atgctttttc
     7261 tgatttcagt attccccaat cagtgtagtg cctttaaac
//
LOCUS       DAZKJR010000201         7290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKJR010000201 DAZKJR010000000
VERSION     DAZKJR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.202"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            85..1236
                     /gene="yiaY"
                     /locus_tag="ACHOWS_004749"
     CDS             85..1236
                     /gene="yiaY"
                     /locus_tag="ACHOWS_004749"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFQ0050217.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAEGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDIPVLATNALKDACGFTNPIQATHEEIMAIYRAAM"
     gene            1366..2661
                     /locus_tag="ACHOWS_004750"
     CDS             1366..2661
                     /locus_tag="ACHOWS_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFQ0050218.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKHATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            2769..4307
                     /gene="aldB"
                     /locus_tag="ACHOWS_004751"
     CDS             2769..4307
                     /gene="aldB"
                     /locus_tag="ACHOWS_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFQ0050219.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4526..4690)
                     /locus_tag="ACHOWS_004752"
     CDS             complement(4526..4690)
                     /locus_tag="ACHOWS_004752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050220.1"
                     /translation="MELLAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGTPMVIYGL
                     VRPLTIETRD"
     gene            4852..5175
                     /locus_tag="ACHOWS_004753"
     CDS             4852..5175
                     /locus_tag="ACHOWS_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFQ0050221.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDVPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            5181..6317
                     /locus_tag="ACHOWS_004754"
     CDS             5181..6317
                     /locus_tag="ACHOWS_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0050222.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(6314..7288)
                     /locus_tag="ACHOWS_004755"
     CDS             complement(6314..7288)
                     /locus_tag="ACHOWS_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0050223.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1830 a         1727 c         1893 g         1840 t
ORIGIN      
        1 taataaacgg taatgttgat catattttca aatgaaaatc ctcagtaagc tgcccgccct
       61 tttttacact ttcaggagtg tgttatggca gcttcaacct tttttattcc ttccgtgaat
      121 gttattggtg ctgattcatt gaatgatgca atgaatatga tggcagatta tggatttacc
      181 cgtaccttaa ttgtcaccga caacatgtta acgaaattgg gtatggcggg tgatgtgcaa
      241 aaagcactgg aagaacgcaa tatttttagc gttatttatg atggcaccca acctaacccc
      301 accacggaaa acgtcgccga aggtttaaaa ttacttaaag aaaataattg cgatagcgtg
      361 atctccttag gcggtggttc tccgcacgac tgcgcaaaag gtattgcgct ggtggcagcc
      421 aatggcggcg atattcgcga ttacgaaggc gttgaccgct ctgcaaaacc gcagctgccg
      481 atgatcgcca tcaataccac ggcgggtacg gcctctgaaa tgacccgttt ctgcatcatc
      541 actgacgaag cgcgtcatat caaaatggcg attgttgata aacatgtaac tccgttgctt
      601 tctgtcaatg actcatctct gatggtcggt atgccgaagt cactgaccgc cgcaacgggt
      661 atggatgcct taacgcacgc tatcgaagcg tatgtttcta ttgccgccac gccgatcact
      721 gacgcttgcg cactgaaagc cgtgaccatg attgccgaaa acctgccgtt agccgttgaa
      781 gatggcagca atgctaaagc gcgtgaagca atggcttatg cccagttcct cgctggcatg
      841 gcattcaaca acgcatctct tggttatgtt catgcgatgg cgcaccaact gggcggtttc
      901 tacaacctgc cacatggtgt atgtaatgcc gttctgctgc cacacgttca ggtgttcaac
      961 agcaaagtcg ctgctgcgcg tctgcgtgac tgtgctgctg ccatgggcgt taacgtgaca
     1021 ggcaaaaatg acgcggaagg cgcagaagcg tgcatcaatg ccatccgtga actggcgaag
     1081 aaagtgaata tcccggcagg cctgcgtgac ctgaacgtga aagaagaaga tattccagtg
     1141 ctggcaacca atgccctgaa agatgcgtgt gggtttacta acccgattca ggcaactcac
     1201 gaagaaatta tggcgattta tcgcgcagcg atgtaatcat cattcccaca atggctgtct
     1261 aattgtcagc cattttttaa actatctcaa aatttgcgat ttttgagata gtttctcttt
     1321 cctgctatac actatctcaa tattgcgctt ttttgagata agcacatggc tatcaaatca
     1381 cctcccggac ttattccttt atcacactta tcgggtgagg aactcctggc ccacctgcgc
     1441 tttaaccggg ttaccgatga aaaaggacgt tatctgcctt ttgatgagct gcagtaccgc
     1501 atcaaaaaag gagaaaacgt cgatgtggcg tggacgctta cccgtctggc gcgcaacgcg
     1561 gcgatccaac gaattaatta ttgcaatgaa gcgggtgaac aggccggatt caacattacg
     1621 ccggtgattg cagaagcgtg cgagcttgtt gataaacatg ccactgcact ggcgctgaaa
     1681 gatcagactg aacggttacg cggggcgggc gccgagttaa gtcagttacg cctggaagag
     1741 ccgatcacca gttcccaact ggaaggtgcg aatactacaa cgctggtggc gagaaaaatg
     1801 ctggaaacag ggcggtcgcc acgcactgaa gacgagcata tgattgcggg aaatgcccgg
     1861 ctgatggcgg aaattccgca cttactcgca gaaccgctga cacctgcgct tattcgccag
     1921 ttacatgcca ttggtatggg tggaatcaat gacgcgaaat atcgtccggg agaatttcgt
     1981 gaaacagatg atgttgtgat cgccgactat gatggcaata tagtgcatca accgcccgct
     2041 gcggcattgt taccggaacg gctggaaaaa gtttgccagt ggttaaacag ccatgaaggg
     2101 tatattcatc cgttggtccg tgcctgcatt ctgcatttta tgctggccca tgaacatccg
     2161 tttcgcgatg gcaacgggcg caccagtcgg gcgctgtttt actggtatat gctgaaatcg
     2221 ggctacgatg tctttaaata tatttccatc agccgtttgc ttcatgctgc accggtcaaa
     2281 tatgccgcca gctaccagta cactgaatca gatggtatgg atctgactta tttccttgaa
     2341 tatcaggcgg gtgtcattaa acgtgctctg caaaactggc agcagcatat cgatgaaatt
     2401 acacaacgaa gcgcaaaact cgatagtgtg ctgtttagct ctggtgtgct taaacgcctg
     2461 aatccgcgcc aggtaacgct cctgaatgtg atgctcgcca atccggggaa agagtacaca
     2521 gttgctgaaa ttagcgcgag cttgggcgtc tctgataata cggctcgagc tgatttacgg
     2581 acaattgtta aagaaggatt cgcgcaagag aaaagaatta ataaccagca agccgtgtac
     2641 gtcgcacact acccgctgta gcttttgccc gtaaattcgt gatagccgtc gtaaagctgt
     2701 taccgactgg cgaagatttc gccagtcacg tctacccttg ttatacctca caccgcaagg
     2761 agacgatcat gaccaataat cccccttcag cacagattaa gcccggcgag tatggtttcc
     2821 ccctcaaatt aaaagcccgc tacgacaact ttatcggtgg cgaatgggta gcgcctgccg
     2881 atggcgagta ttaccagaac ctgacgccgg tgaccgggca gctgctgtgc gaagtggcgt
     2941 cttcgggcaa acgagacatc gatctggcgc tggatgctgc gcacaaagtg aaagataaat
     3001 gggcgcacac ctcggtgcag gatcgtgcgg cgattctgtt taagattgcc gatcgaatgg
     3061 aacaaaacct tgaactgtta gcgaccgctg aaacctggga taacggcaaa cccattcgcg
     3121 aaaccagtgc tgctgatgtg ccgctggcga ttgatcattt tcgctatttt gcctcgtgta
     3181 ttcgggcgca ggaaggtggg atcagtgaag ttgatagcga aaccgtggcc tatcatttcc
     3241 atgagccgtt aggcgtggtg gggcagatta ttccgtggaa cttcccgctg ctgatggcga
     3301 gctggaaaat ggctcccgcg ctggcggcgg gcaactgtgt ggtgctgaaa cccgcacgtc
     3361 ttaccccgct ttctgtactg ctgctaatgg aaatcgtcgg tgatttactg ccgccgggcg
     3421 tggtgaacgt ggtcaacggc gcaggtgggg aaattggcga atatctggcg acctcgaaac
     3481 gcatcgccaa agtggcgttt accggctcaa cggaagtggg ccaacaaatt atgcaatacg
     3541 ccacgcaaaa cattattccg gtgacgctgg agctgggtgg caaatcgcca aatatcttct
     3601 ttgctgacgt aatggatgaa gaagatgcct ttttcgataa agcgctggaa ggctttgcac
     3661 tgtttgcctt taaccagggc gaagtttgca cctgtccaag ccgtgcttta gtgcaggagt
     3721 ctatctacga acgctttatg gaacgcgcca tccgccgtgt cgaaagcatt cgcagcggta
     3781 acccgctcga cagcgtgacg caaatggggg cgcaggtttc tcacgggcaa ctggaaacca
     3841 tcctcaacta cattgatatc ggtaaaaaag agggcgcaga cgtgctcact ggcgggcggc
     3901 gcaagctgct ggaaggtgaa ctgaaagacg gctactacct cgaaccgacg attttgtttg
     3961 gtcagaacaa tatgcgcgtg ttccaggagg agatttttgg cccggtgctg gcggtgacca
     4021 ccttcaaaac gatggaagaa gcgctggagc tggcgaacga tacgcaatat ggcctgggcg
     4081 cgggcgtctg gagccgcaac ggtaatcttg cctataagat ggggcgcggc atacaggcag
     4141 ggcgcgtgtg gaccaattgt tatcacgctt acccggcaca tgcggcgttt ggtggctaca
     4201 aacagtcggg catcggacgc gaaacccaca agatgatgct tgagcattac cagcaaacca
     4261 agtgcctgct ggtgagctac tcggataaac cgttggggct gttctgatat aagaagctgg
     4321 tcgcattggg tattcattgc ctgatgcgac gcttacgcgt cttatcatgc ctacggggac
     4381 ctgaccgtag gccggataag gcgtacacgc cgcatccggc ataaacaaag cgcacgttgt
     4441 tacaatttac gttttaaacc tacgtgcgca ataacccaga ctacgtcaac agaagtgcgt
     4501 ttctggggcg tgttttgcca ggagtttaat ctcgcgtttc gatggtgagc gggcgaacca
     4561 ggccataaat caccattggc gttccgaggc caatcagcaa taaacggtag ataattgaag
     4621 gtttaatacg cttaatctgt actgtggatg ttttactgga gttattcatt agtttggcca
     4681 atagttccat tatttcaata tgataagcct cgcactgttg aaaagccagg ctgcacactt
     4741 cagaaagtat ataaaaaaca atatgtcgtt atataaataa ggaaattgca ccgcattatg
     4801 gtatttcata atatgcgtta attgttcttt tctatattct ctgagggaaa tatgttcctg
     4861 gattattttg cattgggagt gcttattttt gtatttctgg tgatattcta tgggattatt
     4921 attttacatg atgttcccta cctgattgcc aaaaaacgta atcatcctca tgccgacgcc
     4981 attcatgttg ctggttgggt gagtcttttt acgttacatg ttatctggcc atttttgtgg
     5041 atttgggcca cgctttaccg cccggagcgt ggatggggaa tgcaaaacca tgactcctcc
     5101 gttgtgcaac tacaacagcg tattgccggg ctggagaaac agctcgctga catcaaatcc
     5161 tcttctgccg agtaatactt atggatttac tgattatttt aacctatgtg gctttcgcat
     5221 gggcaatgtt taagatcttc aaaattcctg taaataaatg gaccattccc acagcggccc
     5281 tgggaggcat atttattgtc agcggtctaa ttctgttaat gaactataac catccgtata
     5341 cctttaaagc gcaaaaagcg gttatttcta ttcctgttgt cccacaggtg acaggcgtgg
     5401 tgatcgaagt gacggacaag aaaaatacgc tgattaaaaa aggtgaggtg ctatttcgac
     5461 tggacccgac gcgttatcag gctcgggtgg atcggctgat ggcggatatc gttaccgcag
     5521 aacataaaca gcgggcgttg ggcgcagagt tagatgagat ggcggcgaat actcagcagg
     5581 caaaggccac gcgggataag ttcgctaaag agtatcagcg ttacgcacgt ggcagccagg
     5641 cgaaagtaaa cccgttttca gaacgcgata tcgatgtggc gcggcaaaat tatctggcgc
     5701 aggaagcctc cgtaaaatca gcggcggcgg aacaaaaaca gatccagagc cagctggata
     5761 gcctggtgtt gggtgaacat tctcaaatcg ccagcctgaa agcgcagctc gcggaagcaa
     5821 aatataacct tgagcagacg atagtgcgtg cgccgagcga tggttatgtg acccaggtgc
     5881 tgattcgtcc gggtacctat gccgcgtcgc tgccgctacg tccggtgatg gtgtttatac
     5941 ccgatcagaa acgacaaatc gtggcgcagt tccgtcagaa ctccttgctg cgcctggctc
     6001 ccggcgatga tgcggaagtg gtatttaacg ctctgccagg caaagtgttc agcggtaagc
     6061 tggcagccat tagtccagct gttcccggtg gagcttatca gtcgaccggc accttacaga
     6121 cgttaaacac agcgccgggt tcagatggcg ttatcgcgac cattgaactg gatgagcata
     6181 ctgatttgag cgcattacca gacggtattt acgcccaggt ggcggtctat tctgatcatt
     6241 tcagccatgt ctcggtgatg cgcaaagtac tgttacgcat gaccagctgg gtgcattacc
     6301 tttatctcga tcattaatct tcgctttccg ccgcttgttc aggccgcagt tgctgcgact
     6361 gagcaagctg gtggcgaatt tcacgcagca tttcatccag cacatactgg acccgccagg
     6421 gctggtattc ccgtttacgg aagatagcca ccagatccag ccaccattca tacgggtgct
     6481 gaaagcaggg caccagcgtg ccgtcttgca ggtcgctctg cacacttttg tctggcgcaa
     6541 acacaatccc caggtgattt cgtgccagct ccagcgcaga ttgcgtgttg tcgcaaacgt
     6601 aatttccctt cacgcgataa tcccgcacct ctttactccc ggcgacgttg aagcgccaga
     6661 tattcgcgtc gtcgatcatc atcgagtcga tcaaaataca ggagtgatgt tcgagttcgt
     6721 caggacggct aatgggatgt ttctcaagat agcgctggct ggcataagcg gttacggcat
     6781 attgggtaat aaaactggca accagcgatt catcttttgg ttgggcgtag ctgattaaaa
     6841 catcacaatc atcgggaaat tcgacgcctt cataaaaggc attacgctca agattgcagg
     6901 tttttagcga taaggtgata tcaccgatat ctttaatttt atcgattaca tgtttggata
     6961 aataggtaat tatgccggtt ggtgcataga tagtgacccg accacgtttc tcatgcttat
     7021 aatctgcaat aaaattatta agctgctcgt ttctgtccag catgtcgttg atatacggta
     7081 acagcacggt gccaaaaggt gtgagcatca gctgtcgggt ggttcggtca aagactttta
     7141 aacccacttt tgattcaaaa tcagcaagat atttgctgac gttggcctgt gcgatgccaa
     7201 gtacagtcgc ggcatggctg atattttcac tggcagcgat taccgagata atttttaact
     7261 cccggtactt aagttgtaat tttgtcatcg
//
LOCUS       DAZKJR010000202         7115 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKJR010000202 DAZKJR010000000
VERSION     DAZKJR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.203"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..612
                     /locus_tag="ACHOWS_004756"
     CDS             16..612
                     /locus_tag="ACHOWS_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0050224.1"
                     /translation="MNSTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TTEILATLPSLKKMGNYLISFTRSHRSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            683..1630
                     /locus_tag="ACHOWS_004757"
     CDS             683..1630
                     /locus_tag="ACHOWS_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0050225.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENHFNLIIKNNVEHLVKSEST
                     VLRESICAVIAGKDCYQADTLSRLPGVKIISRFGTGIDNIDLHTARQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(1932..3308)
                     /locus_tag="ACHOWS_004758"
     CDS             complement(1932..3308)
                     /locus_tag="ACHOWS_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFQ0050226.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LSGQFRNAALRLERLAPQGAETIITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3308..3736)
                     /gene="tssE"
                     /locus_tag="ACHOWS_004759"
     CDS             complement(3308..3736)
                     /gene="tssE"
                     /locus_tag="ACHOWS_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFQ0050227.1"
                     /translation="MATPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHSLMRQIEETLLRYEPRLNQIQVELLPQPGPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3740..4276)
                     /gene="tssJ"
                     /locus_tag="ACHOWS_004760"
     CDS             complement(3740..4276)
                     /gene="tssJ"
                     /locus_tag="ACHOWS_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFQ0050228.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(4257..5336)
                     /gene="tssG"
                     /locus_tag="ACHOWS_004761"
     CDS             complement(4257..5336)
                     /gene="tssG"
                     /locus_tag="ACHOWS_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFQ0050229.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5300..7060)
                     /gene="tssF"
                     /locus_tag="ACHOWS_004762"
     CDS             complement(5300..7060)
                     /gene="tssF"
                     /locus_tag="ACHOWS_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFQ0050230.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSNFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQERRVLDAHFS
                     PMGFDDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPEGMSGFEIDV
                     VLSENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDSDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLSAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSTGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1680 a         1856 c         1871 g         1708 t
ORIGIN      
        1 tcaaggaaaa taaaaatgaa cagtacggat cttatccatc tcattaaaca ttttatgcgt
       61 aacgaacata aagcagttga agaagttatt gattcccccc tttcggaggt cgcaaattta
      121 attaaagtat tacagtcttg tcaggggaaa gttgttttta ttggtgtcgg taagtccggt
      181 attattgccc gaaaactcgc cgcaacattt gccagcactg gaactccctc gttttttgtt
      241 cacggtactg aagccgtaca cggcgatctt gggatggtgg cgaaagatga tgttgttatt
      301 cttatttcaa acagtggtga gacgacagaa atcctggcaa cactacctag tctgaaaaaa
      361 atgggtaatt atttgatctc ctttacccgc agtcatcgct catcgcttgc gataagttgt
      421 gatttatccg ttgaaattcc cgtaaaaagt gaggcggata atttaggttt agcgccgtca
      481 tgctcatcaa cagtcgttct ggttgttggt gatgcggtgg cgcttgcgct ctccgaattg
      541 aaaaagttta ctcgtgctga tttcggctta tatcatccag gaggtgcgct cggtattaaa
      601 gcaaactcat aattttagaa tataaaacag taaccggtca cgccatcttt tggggtggcg
      661 ttcattgatg tgagtgtcag ctatgaaaaa cgttctggtc accgttccca gcttttcagc
      721 gcgttgtgtt tctgccagca agctgctaag agaaaatcat tttaacctta ttatcaagaa
      781 taatgtggag catctggtta aatcagaatc cacagtatta cgagaatcta tttgcgcagt
      841 cattgccgga aaagattgtt atcaagcgga tacgttatcg cgactaccag gagtgaaaat
      901 aatatcgaga ttcgggacag ggattgataa tattgatctt catacggctc gacaatcagg
      961 tattgtggtc aataatgctg ttgggattaa ttcaaatgca gtcgcggaat ttatcatcgg
     1021 tcttattttt gcgagcatga gaaatatccc cggtagctat catgcaatgc aaaatggtta
     1081 ttggggtgag tcgcatggct gcgagttaca aggtaaacgt attggcctgc ttggctacgg
     1141 caatattggc aaaacactgg cgaaaaggtt atctggtttt gatgttgagc ttttagcttt
     1201 tgacaaacaa cccgactatc aggttgctga caaagctgga gttcagtttg tatcaattga
     1261 agatatcttt atgcagtcgc atgtcatcat tgttcttttg cctttttctt ctgagctgga
     1321 gaacttcatt agtcataaat atttgtcgat gatgcgcaat ggtgctctga tcatcaatgc
     1381 cgcaagggga aagttgctgg atgagggcgc tttacttcag gtgattgaag aacgaaatgt
     1441 gtttgcggcg ctggatgtgt ttagctctga accgttggcg caatttagcc cactgctaca
     1501 tgccaaaaat atcattacaa cgccgcatat cgcggccgcc accgttgagt cataccagca
     1561 aaccgggata cacgttgccc agtcgataat tgattacttc gcaggaaggg agataaaaaa
     1621 cgtgttgtga gttcagcgaa caaagatttt aaatacaaaa aaaccgcaat ttgtagaaca
     1681 cattgcggtt tgtcagcatt ctaatcaatt ggttgcgggg gccacagtca agaggttaat
     1741 ccaatctttt gaaacttaag gtattcactg ttcccgaacc caagctatct accgaaatat
     1801 acactcatta tctgatgtga atcaacgagt agatcgaatg gcctgttcct ctcttttttt
     1861 gtacacagag actccatttt aaaaaatggc aaggtcacat atcccttatt ggtacataaa
     1921 tcccccgatg tttactgact ttgttccacc ttcgtatcca gtaaaatgac atagcgattc
     1981 aacaactgat ttaaccgggt atcaatttgc gtcaacagcg ttggcgaaac ctctccggtc
     2041 tgtttttgct cttccagttg ccgaagcagt tcttccagcg gcggctcctt taacgactgc
     2101 cggatgccgt acaccgccgt ttttagttcc gaaccggtca gatatctccc cttacgttca
     2161 tccagtgcat ccagctgcgc cgacaggcgc tgtagctggc tctgcgcctg agcatagcca
     2221 ttcagtgcct cagcagacag ggcgctcagc tcccgctgtt tgcgccactg cgcgttcagc
     2281 gccctgacct gaggattatc cggccacagg gcatcaagct ggcgtagctg gcgatatccc
     2341 tgctccagtg gccagcgggc aggcaatttg tttaactgct ccagttgcat ctgacttgcc
     2401 gcaattgttc tgtcctgagc cagcagcgcc ttatccttta catccagctt tgccagttcg
     2461 ccgctctcca gcgactgcgg caacggcatg acagacagtt gtattcgctg gattagcgcg
     2521 tccggctgat taagctgcga ccagccccat aatcctgttc cgccgagacc ggccatcaca
     2581 accattccgg ccataaatat cggccatgtc cgctgccggg agatttcagg ggatggcacg
     2641 ggtggactgg catccttatc gttttgctga acggatctgg ttgcaaaaac cggctgtgga
     2701 ctttcctctg actttttcgg ttgattcatt tcccggcggg gtaattcagg gggagtgata
     2761 atggtttccg ctccctgagg cgccagccgt tccagacgca gagccgcatt gcgaaactgc
     2821 ccggacaata cgtccagctt cgtcatatgc cacagttcac actgttgcag cgtggtttcg
     2881 atggcggaaa gatgctgtac acaacggtag atctgcggca gatcctgata ctgaatatcc
     2941 agcgttctca gtgcctgctg cattttttct gtgacccagc tgagcagtga tattctggaa
     3001 tgtacctgta ccggccagaa gtcagcccag cgctggagca gtatatccag tgaaccgagt
     3061 ccgtctgcca tccctgccag cccctgccgt ctggttattg ccagcacgta acagaccagc
     3121 gtctgtagtt ccacgccatt ttgccggaac agcgcgaggc agagtttttc cacctgacgc
     3181 cagtccacat ccggtcgggc cgggtgagtc agtttactca tttcagcctg cagttcaccg
     3241 gattccttat aagtacaggg atcgccgccc atgacgaact ggctgataaa attcgcgtta
     3301 cttgccatca gtacgcccgc tcctgttgtt tgagatgacg cagaacaatt cgtccttccg
     3361 gagaaaatac gccatcaaag cgtatccagc cggtatcggg aagctgcgcg tggatcaggt
     3421 aattaagatg ccccggaccg ggctggggca gtaactccac ctgtatctga ttcaggcgtg
     3481 gttcataacg cagcagcgtt tcttcaatct ggcgcatcag gctatgaata cccgcagcca
     3541 tcccctgatg gatcaggctg agatccggca gaccgtaatc cggcaaatgc gccagtgtgc
     3601 ctgcccgaca gttgataatg cgttgcatat tgtccatcac ggaaaggata agctgatccc
     3661 gctcactgac ctgttccagc ggtaactcgc cgctgaaact gaacgtcagc atttcatata
     3721 aggaaggtgt cgccatcggt tatttatcct tgagaggcag cagtcgcagg tcgcttctca
     3781 tcagttcaat cgagcgcggc ttgtcggcct ccagttcgtc ccttttgatc accagacgcc
     3841 agctgtcgct tttttcatcc gggtgataaa actgcccaat aatggcgaca aactgcgtct
     3901 ctttatccag cggcacattc agggatgccg tgccttcagg ttttacccag acggtgtgtt
     3961 ttgccagtac gtccttctcc agcgtcttct cttcctgcat aaacagggtg tcataatccg
     4021 ccttatcaaa atcttcgcgg gttttcagtt gccagacatg caccatcgtg gaaagcggcg
     4081 tatcttccgc atccgtattc agggcgctgc gggagaagaa atcgagatgc agtattttga
     4141 tttgtttgta gaaaacggct gaggccacgc ttttcgattc atccaccact ttctgaatca
     4201 gcccgcagcc actcagaaca accgtcagca tcgccataca gagcaacgcc ggacgtttaa
     4261 aacgaatagg aaacatgagt catactctcc ctgttacgaa tggaaaaagc gggtattaat
     4321 ccctgataac gccccagact gaccgtcact gttccggtcg cgggcgcggc aaaggaggcg
     4381 cgaaggatgc cggtacggct aatctgtaca cgctgttttc ccagccgggc agcaggcaac
     4441 aggctgacgg gcagcgtcag ttgcagacgg gcctgacagc gccagcccag ataaacgcgc
     4501 agcagcacca gcaaatcgct gtgcagttgc ccgccgggca gccagccacg tgcttctgcg
     4561 gcatcttcgg tatacagttt cagcagcaac tggctgttga cgtcggtacc ggtacgcccc
     4621 agcagcgttc gcgttttcag gctgatgcgt gaattttttg acagacccgc aggggccggg
     4681 agaattacag gctgcggatc gtgaggcacc accgtcgctt tggtcagcgg ggcaagcagc
     4741 cctaccagtg ccgtgacgcc ttccgccgtg cgcgtcggca ggcgcatcac gctgagcagg
     4801 gccagaaaac gggataccgg cgtggcgata tggttctcgc tgccgggaat acccagcccg
     4861 atgagtccca gcagacaacg ggaaatgtca tccacggcac ctgcttcaaa cgacgccggg
     4921 tagttatatt tgcgccagat gcggtagtac tgagtaataa agcgatggtt aaaaatatcg
     4981 agaaacgcca tcactgcgtc atgtccgtca tgacgctggg taatgtagtc cagataggcg
     5041 gttggcagcg gcgaatccac gccgtacagg ccgagaaaag tggtgcgcac cgtggggggc
     5101 gcgtcgggat tgcccacgtc acgttcaacg ttttttaact cgctgaccgg aaagcccata
     5161 cctggatgag gacgaaagcg caccgcatcg gtggcggggc tgtcggtact gcccagcggc
     5221 gcgttctgct ggctctgttc cagcaactgg caaaaacggt aaaaattgat ccgcgcaata
     5281 tccggggcca gccggtcggt cagcccggta cgcgctggct gtgatgctct gtccattgca
     5341 ggcactttcc tgtgggttgc agaattaagg tcagttgagt gaataagtgg atatcggcat
     5401 acagtgcaaa gaagcggtgc agcagttcgc caaacagcgt gatatcaccc tctccggcaa
     5461 agccggtgct gtccagcgtc acctgaatat ccacgccacg cagcagaaag cctctttcaa
     5521 aacgctgtat caggctgtgc tgaacgtcga caatcgccga cagacggcgg cggttcatct
     5581 cgctttcggt ccagtcgtac agcgccagcg tgccgcgcag aatttcggcg ttatccatca
     5641 ttgaaagaaa gttagaaccg aggtggctga gcacccgcca gtggaagcgg tcacgcgccg
     5701 ggggataaca gggctgcgtc ggggcgcaca ggttgcgcac ccgcaggacg ttctgtgagg
     5761 catgaactgg cgtatcaagc agcgtgctct gtaaagcctt acggggaagc tgtccgttag
     5821 tgccggtcag gcttaaagag agcgtttcat cctccagcat ccggtcggaa tcaaacgcat
     5881 cgccgcccag aatcagccag gtatcatgca ggccggaagg gccgcgctta acccgcgtat
     5941 ggtaatagcg ttccggtgca tcgtggcgca gcatcccgcc ccggtgacga aagctggaaa
     6001 acgggacata cgcctgactg ccggtatggc gcgaagaaat aatgttgtcg acggaataaa
     6061 tctcggtatg cccgtcctga atacgcatcg ggcgcagcag aaattcgttt tccagtggcg
     6121 aaaggtgtaa cggatcggct tccagcggga acaggttgat gaccggcacg cagtgcagac
     6181 ggatattatc gctgtcaaac ggcaggtcgt gaggccagtt ctcgctcagc accacgtcaa
     6241 tctcaaagcc gctcatccct tccggccaga tgacgttctc cagcccgttc agcgccacaa
     6301 acataaactt ttcgcggaag gtgaaatact ccagcagcaa ctggtagccg ctgaacgccg
     6361 actcgccttt tggccacagt cggtcattgt catcaaaacc catcggggaa aagtgcgcgt
     6421 ccagcacacg gcgttcctgc cccggcagac gcagccagaa ctgctgggtg ccaagcgtta
     6481 aagcccggtg cagcgcacag gccaccgggc tgtcggcatt gagatagagc ggcaaacggg
     6541 atagatcgat ctgtgaccag tcaccaacca gcgggccgca ttcaaagcgc agacggataa
     6601 ccgagcgccc gtccggttca tattgcaatg acacgtccgg cagcgccagc ggatgcagcg
     6661 tgatatcccg cgtagcactg tagacacagc gggttctgcg ctcgccaaca ggacgcgaca
     6721 atacctgaaa attactcagt gtttcgctct gcttcatctg ccggtgatct gtcgacagtt
     6781 caaccacgga aagtgacggg atggttcgca ggtagtgagg ccacagcagg ctgaccagcc
     6841 cctcggtcag ttcgggcagg tcgtcatcca gcttttcacg caggcgaccc attaaaaaag
     6901 cgaagccttc gaacaggcgc tccacatagg gatcgcgtgc gccgggttta tcaagattga
     6961 gcatcgcggc gcggtcgggg taagcctgcg caaattcttt ccccgcttca cgcaggtagc
     7021 gcatttcggc ttcgtagtag cgttgggtta gatcgtccat cggagggatt atcttccttt
     7081 cattgaaagt gaatatcatg caggtgtaga agtaa
//
LOCUS       DAZKJR010000203         6995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKJR010000203 DAZKJR010000000
VERSION     DAZKJR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.204"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1500
                     /locus_tag="ACHOWS_004763"
     CDS             <1..1500
                     /locus_tag="ACHOWS_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0050231.1"
                     /translation="VDTTITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVY
                     YRTFERLPVIELSNGRVVAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIV
                     DRPCSAPQGMFPLVPFWGYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVI
                     MDEDATQLSDSDLMEQIERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQE
                     SEKLIQDTMGVYSAFLGQDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLL
                     AYLLDDLKKRRNHAVVINRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQT
                     PAFKAQLAQRMSEVIQGLPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDE
                     MTPEEQEVAAQQQALQQQQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVAL
                     TQGQRYVDALNQAHTAEIITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            1658..2665
                     /locus_tag="ACHOWS_004764"
     CDS             1658..2665
                     /locus_tag="ACHOWS_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050232.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            2689..3903
                     /locus_tag="ACHOWS_004765"
     CDS             2689..3903
                     /locus_tag="ACHOWS_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFQ0050233.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            3959..4348
                     /locus_tag="ACHOWS_004766"
     CDS             3959..4348
                     /locus_tag="ACHOWS_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050234.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            4398..4859
                     /locus_tag="ACHOWS_004767"
     CDS             4398..4859
                     /locus_tag="ACHOWS_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050235.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFCRRVAETEQHGGTE"
     gene            4843..5406
                     /locus_tag="ACHOWS_004768"
     CDS             4843..5406
                     /locus_tag="ACHOWS_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050236.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            5406..6056
                     /locus_tag="ACHOWS_004769"
     CDS             5406..6056
                     /locus_tag="ACHOWS_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFQ0050237.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLSCVPGARQTLPDGAIQLLDVICLSDGSAIKPVSREVLDAQYPDWHMLRG
                     KPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            6053..>6995
                     /locus_tag="ACHOWS_004770"
     CDS             6053..>6995
                     /locus_tag="ACHOWS_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFQ0050238.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGD"
BASE COUNT         1727 a         1623 c         2097 g         1548 t
ORIGIN      
        1 gtcgatacca cgattactga aggccagccc agtccgttga tgagtgcatg ggaagagtat
       61 cagtcatggg atcgcaggga gaatgaatgg cttcagcgtg aacgccgtcg tgtgctgctt
      121 caggtggttt attaccgtac attcgaacgt cttccggtga ttgaactcag taatggacgg
      181 gtggtggctt ttgataaaaa taatctgatg caggcggtgg ctgtggcatc cgggcgggtt
      241 caggtgaaag tcgggcgggt aagtcgtatt cgtgaagcct ggtttgtggg cccacacttt
      301 attgtggatc gcccctgtag tgctccgcag gggatgtttc cgctggttcc tttctgggga
      361 taccgaaagg ataaaaccgg ggagccatac gggttaattt cccgcgccat tccggcacag
      421 gatgaggtga attttcgtcg tatcaagctg acctggctgc ttcaggccaa acgcgtgatt
      481 atggacgagg atgccaccca gttgtcagac agcgacctga tggagcagat cgaacgtccg
      541 gatggcatta ttaaactgaa tccggcccga aaaaatcaga aaagtgtcgc agatgttttt
      601 cgggttgagc aggattttca ggttgccagc cagcagtttc aggtcatgca ggaatcggaa
      661 aaacttatcc aggataccat gggagtgtat tccgcatttc tcgggcagga ttcaggtgcg
      721 acgtcaggcg tggctatcag taacctggtg gagcaggggg ccacaaccct tgcggaaatc
      781 aacgataact accagtttgc ctgccagcag gtgggaagac tgttgctggc ttatcttctc
      841 gatgacctga aaaaacgccg taatcatgca gtggtgatta atcgcgatga tcgccagcgt
      901 cgccagacca ttgtcctcaa tgctgaaggt gataatggtg aactgaccaa tgatatttca
      961 aggttaaata cacatattgc gctggcgcct gttcagcaga caccggcgtt taaggcacag
     1021 cttgcacaga gaatgtcaga ggttattcag gggctgccgc ctcaggtgca ggctgttgtg
     1081 ctcgacctgt gggttaatct tctggatgtg ccgcagaaac aggagtttgt tgagcgtatt
     1141 cgtgctgcgc tggggacgcc aaaatcaccg gatgaaatga cgccggaaga acaggaagta
     1201 gcggcacaac aacaggcact tcagcaacaa caggcagaac tccagatgcg cgagatggct
     1261 ggcagagtgg caaaactgga agctgacgcc gccagggcac atgcagctgc acaacgggat
     1321 aatgccagtg cacagcggga agtcgccctg acacaggggc agcgttatgt ggatgcgctt
     1381 aaccaggcac atacggcaga aatcattacc ggcgtacaga atatggaaca ggagcaggac
     1441 gttcttcagc aacagatgct gtatacgtta caacagcgga tgaatgaaat gtcgctctga
     1501 aaactctggc ttcaactgaa ccccgtcatc gtacggggtt ttttgtttcc ggaggtaagc
     1561 gttccgggag cggtgcgctt attcgcgggg gcagcgataa gccttattta ctcaaccatt
     1621 cggatctgtc cgataaacag accatgcgga gttatttatg gattttgaat ttacgggtga
     1681 agaaaccccg gaacaactgg aaaaaatgct ggaaggactt ggggatgtgg atattgacag
     1741 tcacgcacag gacgtcgtga cggaagatac cacggaaaaa catgcggatg aggaagcaca
     1801 gactcagacg ggcgataaca atgtggcacc gacgccggat gccagtgtgg agcagacgca
     1861 ggacgtgaag gagccggaag cgaagggggt gctcacccgc gacggtaaac acgtcattcc
     1921 ctatgaagtc cttgaggctg aacgttccgg taagcaacgg gccgaacagg aagccgcact
     1981 tcttcgtggg cagatagctg aagaaaaacg cagggtggaa ctgctgacgt ctcagatcca
     2041 ccaggccggt atgaagccca caccgttacc ggaaaacgaa aaaatttctg atgagcagat
     2101 tgcccgtatc agggagatgt atccggaaat tggtgacgcg gtggcttcgc tcatccgtaa
     2161 aaataactat ctccagtccc gtgttcagca atcaacacag caggcagaag gtaatggtgg
     2221 tgaggattta tcaccggttc ttgatgcgat gaatgccgtg ccggtgctga aaacgtggca
     2281 ggagtccgat ccggatcgct tctcggttgc tgtatccatc gacgggaagc tccagaatga
     2341 ccccgcatgg aaagacaaaa cgctcactga acgtttcgct gaagtggccc gtcgtacgca
     2401 ggttgctttc ggtgaagtca gtgagtcgtc tgctgacaac caggcagaca aaacggatat
     2461 ccggaaaacg gcggaagaaa aagtgaagac cgctgaacag gagcaggcag tacctgcttc
     2521 cccgtcagat ttaggcacca cggcctccgt cggaaccggt gataattttg aacggttact
     2581 tggtgcttct cattcagagg ctgaggcgat tatgcgtggt atgacgaatg ctgaaataga
     2641 cgcacttctg gagaagctcg ggtaacttac tgaaggagta ctgaagtaat gacgactgta
     2701 acatcagccc aggcgaataa gctgtatcag gtggcgcttt ttactgctgc caaccgcaac
     2761 cgctcgatgg ttaatatcct tactgaacag caggaagcgc caaaagcggt ttcgccggac
     2821 aagaaaagca cgaagcagac cagcgcaggt gcgccggttg tccgtatcac agaccttaac
     2881 aaacaggccg gtgatgaagt gaccttcagc atcatgcaca aactctcaaa acgtccgacg
     2941 atgggagatg agcgtgttga aggtcgtggt gaggatctca gccatgctga cttctccctg
     3001 aaaatcaatc agggacgtca cctggtggat gcaggcggac gtatgagtca gcagcgcacg
     3061 aagtttaacc tggcatcctc tgccagaacg cttctgggga cgtactttaa tgacctgcag
     3121 gaccagtgtg cgatagtgca tcttgctgga gctcgtggtg attttgttgc tgacgacact
     3181 attctgccga cagcggagca ccctgaattc aaaaaaatca tgatcaacga tgtactgcct
     3241 ccgacacatg accgtcactt ttttggcggt gatgcgacaa gctttgagca gattgaagcg
     3301 gcagatattt tttctattgg cctggtggac aatctctccc tgttcattga cgaaatggcg
     3361 catccgttac agccggttcg tctgtccggt gatgaacttc acggagaaga tccatattac
     3421 gtcctgtacg tcacgccgcg tcagtggaat gactggtaca cctcgacgtc cggtaaggac
     3481 tggaaccaga tgatggttcg ggccgtgaac cgtgcaaaag gttttaatca tccgctgttc
     3541 aaaggtgaat gtgcgatgtg gcgcaatatc ctggttcgta agtatgcggg tatgccgatc
     3601 cgtttctatc aggggtcaaa ggttctggta tcagagaata acctgacggc aaccacgaaa
     3661 gaggtcgctg ctgcaaccaa tattgaccgc gccatgttac tgggggctca ggcgctggca
     3721 aatgcttacg gtcagaaggc gggcggtcac ttcaacatgg ttgagaagaa aacggatatg
     3781 gataaccgta ctgagatagc aatcagctgg atcaacggtc tgaaaaaaat ccgtttcccc
     3841 gagaagagcg gcaagatgca ggatcacggc gtgattgccg ttgatacagc agtgaagctc
     3901 tgattttttc ctttccccat gccgggtttt cgcccggctt tttcaggagt cattaattat
     3961 ggcaaagact atccttgccc cgtcactgag tgaacgggtc tatacgggta cgcacggtaa
     4021 tgagtcggtg gcagaaggcg tatttacggt gaatgctgcg gaagcggaca gtgttattca
     4081 tcttctctca ctgccagtgg gcatccgtat caactcactc cagctggttt caacgggtgg
     4141 tctgggtact gcaaccgtca gcattaagtc cggtgagcat gctctcatcg ataacagcga
     4201 agctgtttct gcaaaatttg ccagatatgt gccagtggag ccgtacacca cacagcgtga
     4261 cggggagctg gttactgtca ccattaagac tgccgctgca accggcaccc tgaatgttct
     4321 gctgcgttat accgtggtgg gatactgatt aaaaccttcc ggcccgcgtc atgcgggctt
     4381 tttatccggg gaattatatg agtgagaaaa ttgccgttgt ctatatcggc ccaaaacccg
     4441 tgaaaaagga caccattacc ggaagccgca cattgttccc acgtcttgag ccggtgcatg
     4501 ttgacagtgc gatggcctgg caactgctgg ggtttccgga tgtctgggtt cgtcatgaag
     4561 agcttgatga tgttctgaaa aagcaacaac agaatgagca gttgcggcag gcacagcagg
     4621 cgcaggaaag agtgcttgct gcgcgggcag aagcggagaa cagttttgtt gtttctgtta
     4681 acgggcagga ggtggattta agtaagctca cctcagcacg gctggcgacg ctgtgtgagg
     4741 cagaagagct ggatattcac aaagacccga aagaaacggc tgaggcattc cgtatccggg
     4801 tgcgtgaggc attttgccgt cgtgttgcgg agactgaaca gcatggcgga actgagtgat
     4861 tttttaccgt atgtccgtcg tcatatcagc ggtccactga acattatgat gacggatgct
     4921 ctgtcaatgg ctgccgtggc attcagccgc cagtcgttgg tgtgccgtcg ggaggttact
     4981 gttgtaccag tagcaggaaa agaaatcgtg cttccgtatg acaaagatga tgaggagtgc
     5041 gttcatatca tccgtatctc tgacgataat catgagcttt ttgtcggtcg ggatgtggat
     5101 atcagctccg gacgctcctt gcgatttgcc tgttctcccg gtgaggtgag cgtgctttat
     5161 gccgtcgctc cgaaagccgg acgcagccag ataccggatg aactcctcac atggcctgaa
     5221 gaagtggctg cgggggcact tgagcggttg ttcatgcaga ctggtgtttc atggtcagat
     5281 ccgttacgcg cacagtattt ttctgtgcag ttttctgagg gtatccgtcg ggcatatcgt
     5341 catacactgg cgacaagtcc gtactcctca taccgcaatc ctgtccgcag acaaaggttt
     5401 tactgatgac gacgattact gaaatcatcg gacgagtgaa cacgcaactg gttgacccga
     5461 tgatggtgcg ctggcctctg gctgagctgt gtgattatta caatgatgct gtgcgggcgg
     5521 tgattctggc aaggccggat gcaggcgcaa gcctggaaac gctcagttgt gtccctggcg
     5581 cacgccagac tttacctgat ggcgcaatac agcttcttga cgtgatatgc ctcagcgatg
     5641 gcagtgcgat taaaccagta tcccgggagg tgcttgatgc acagtatccc gactggcaca
     5701 tgctgagggg aaaaccggaa tgctttatca gcaacgacct gtccccgcgc gtattctggc
     5761 tgtttcctgt ccctgacaaa gagataagta ttgatgcagt ggtaagccgg ataccggagg
     5821 cagtgtatgt tctgacgcag gacgatgata cgccagttcc actggaagag gcttatgtta
     5881 acccactggt ggactggatg ttgtttcgtg ctttcagtaa ggatgctgcc ggtggtgcag
     5941 aatcggggct ggctgcgcag cattatcaga gttttgttga gcaacttggg atcaaacagg
     6001 gggcagacag tgcattgtct gcccgtaaaa aagtgtttaa cggaggtgga gtgtgagtgt
     6061 tgttgtttcg gggacgctga aatctcctga tggtgaggcg atatcaggag caaatattac
     6121 cctgacggcg ctgacagttt caccggatgc gctcagcggc accagtgcgt cggcagtgac
     6181 ccgtgaaggt ggatattacg gaatgacgat ggatccgggg gagtatgcgg tttcggtgac
     6241 ggtgaaaggg aagactgctg tctacggacg tgtgcgtatt gaggggaccg aaagtacggt
     6301 gacgctcaat atgctgttac gccgcagtct tgttgaggtg agcatacccg gagagctgct
     6361 gacagatttc cggcagatac agaataatgt ggctgatgac cttgctacta ttcgtcgcct
     6421 gaatgaagac acggcgacaa aaaacactca ggccacacag tcaaaagaaa gtgcagcagc
     6481 cagtgcgaag agtgcatctg acagtgcaaa gacggcaacc agcagggcgg ctgaagccgg
     6541 acaaaaagcg actgatgcca ctgaggccgc gacccgtgca atcacagcag cggggaatgc
     6601 agaggaaagc tcgacccgtg ccggagagtc tgaaaaagcc gccggagctg atgcagaaaa
     6661 agccagacag catgctgaaa aggccaggct ggcgcaggag agcgccggag agatccttaa
     6721 gcgggcagag gctgccactg tcagtgctga agaggccaga cgtatggctg agaatgcacg
     6781 ggggccccgg gggcctcagg gagaaactgg tccgaagggg gatgtcggtc ctaaaggcga
     6841 aacaggtcca gtgggccctc aagggcccgc agggccgaaa ggtgagcgtg gtgacgttgg
     6901 tgctcagggg gctgtagggc ccgctggtcc gcgtggtgag aagggcgaac agggggagcg
     6961 aggaccgcag ggaataccag gcctgaaggg ggata
//
LOCUS       DAZKJR010000204         6856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKJR010000204 DAZKJR010000000
VERSION     DAZKJR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.205"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2863
                     /locus_tag="ACHOWS_004771"
     CDS             <1..2863
                     /locus_tag="ACHOWS_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFQ0050239.1"
                     /translation="RIRNQGKENEYIAVKLPGDKKFTNLKETIFSDDFIVRRELKKPP
                     LEKSVIQQRLHEWPQRAMELKYIDKANSDFRKRYYSSSQEEKIKILAQQETEFYQKYG
                     ERYDVFPAERKTNHKSGINEVETRGTTLSPNGLQSVSSGEMATGWQTGNTGSPLFLST
                     DALVFMGQSQSTGRDFGLRRDVHPGGRGRSGNQRGSGQPATGTNATVHSRRSTRTQYG
                     NRYAGGLLAGNVNIPVYARNKPGGATIQDVIRRSEILLRPPPGKSNISVNRFNGDIPQ
                     MGKNAAYVAARFVRQYEQNQILPYQRQALWDIRQLYQSTRSFIWQDDRLTRKERSQYL
                     SVLAFEHIKARQKIINSSQQENTMASEDIRKLIKKKPRLSNNEITGKDERNIPPAGKR
                     FSRIVHTLKNQIEGEDKENSYAKSVSAADLYTRKSKLSKNVHYLDKNSNRTLFVDTGN
                     AIAIRKEGMSPAALGIALELAKERFGSTLTIKGGEEFRNQVIDIVARNHPDIHFSDKV
                     MNRQLEERRAEIAAERDGMTIEGVSKTSTNKEQEKEHAQAADTKNQTNVKESSEPHES
                     PESRTVVKLEGKLIDHGKAPLDNKKGAEQSYFVTVRGRDGKETTHWDSDLQNVMKGKR
                     RGTEISLELTKSNPVQVKTRDHQGNLSIREAVRQTWTLNKMPVGQEQKRKQSLDKTQQ
                     NQSSTPAKTQTAVDLILANPVLRQMAMTAEQKEREFTALQADKESVREQTDQEIRNRL
                     GAGDQSKEPLAVILSDDELRGMALSMEQKMREMATPVDKLDAVKAEINSEIAARVARN
                     SSIPVPVSEVSATVTQKEIHREAESRGKKDKVHEGVLLDHGRAAYKFKPDISKPEDER
                     VDSYFVKLKMDNGKTRTLWGVGLESVVAGYNKGERLRLEDKGTEKVQWIEKMKDGREI
                     EKNGDRRVWDCRSLTRESEVMKGFRVSGMPERDDGPDVA"
     gene            3263..3526
                     /gene="repA"
                     /locus_tag="ACHOWS_004772"
     CDS             3263..3526
                     /gene="repA"
                     /locus_tag="ACHOWS_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFQ0050240.1"
                     /translation="MSDAVRSATSSEARPKRAYRKGAPLSVNERKRLSLNRKRDTHKA
                     LNVFIRNELKDILERFCQDKHLTLGEGIELLIQQEKERAKNGE"
     gene            complement(3579..3662)
                     /locus_tag="ACHOWS_004773"
     CDS             complement(3579..3662)
                     /locus_tag="ACHOWS_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFQ0050241.1"
                     /translation="MIGVFAFLAPVNPHQNQFPATLRRGQP"
     gene            3750..3824
                     /gene="tap"
                     /locus_tag="ACHOWS_004774"
     CDS             3750..3824
                     /gene="tap"
                     /locus_tag="ACHOWS_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFQ0050242.1"
                     /translation="MPGKVQYFFLCSLLLRIVSAGWCG"
     gene            3817..4674
                     /gene="repA"
                     /locus_tag="ACHOWS_004775"
     CDS             3817..4674
                     /gene="repA"
                     /locus_tag="ACHOWS_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFQ0050243.1"
                     /translation="MADLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAAHVAYARSRGLRRRMPPMLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESEAGKLSITRATRALKFLAELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQAELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            5592..5864
                     /locus_tag="ACHOWS_004776"
     CDS             5592..5864
                     /locus_tag="ACHOWS_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0050244.1"
                     /translation="METYIRTRVDDVLKSQFETILQDCGLSVSAALRLFAENVVRNEG
                     LPFEITRKPSARLRESIRQTEELMAQGRPGFENVGELIASMNNGEQ"
     gene            5851..6156
                     /locus_tag="ACHOWS_004777"
     CDS             5851..6156
                     /locus_tag="ACHOWS_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HFQ0050245.1"
                     /translation="MGSNKRAPLPYRTDRTKNFEKAWKRYNKSGRYDMQAAAVAMNLV
                     TNRNPVPAEYRDHELTGEWAGFRELHIGGDFLLVYRVDEKNEIIYFTDLGTHAELFE"
BASE COUNT         2053 a         1480 c         1696 g         1627 t
ORIGIN      
        1 ccgaattcgt aatcagggaa aagaaaatga atacatcgct gttaaattac ctggtgataa
       61 aaaatttacc aatctgaagg agacaatatt tagtgatgac tttattgtga gacgagaact
      121 gaagaagcct cccctggaaa aatctgtcat tcaacaacgt ttgcatgaat ggccacagcg
      181 agctatggag cttaagtaca ttgataaggc taacagtgat ttccgaaaaa gatattattc
      241 atcctcgcag gaggagaaaa taaaaattct tgcacaacaa gaaacagaat tttatcagaa
      301 gtatggagaa agatatgacg tattccctgc cgaacggaaa acaaatcata agtccggtat
      361 taatgaagtt gagaccagag gaactaccct ctctcccaat ggcttgcaaa gcgtgtccag
      421 tggcgaaatg gcaactggtt ggcaaactgg aaacaccggg agtccgttgt ttttgtccac
      481 tgatgcactt gttttcatgg gacagtcgca atccaccgga agagattttg gactgcgacg
      541 cgatgtacat ccaggaggaa gaggacgatc tggtaatcaa cgaggaagcg gacaacccgc
      601 taccgggacg aatgccaccg ttcactccag acggtcaaca agaactcagt acggaaatcg
      661 atatgccgga ggattactgg ccgggaatgt gaatattcct gtgtatgcgc gtaataaacc
      721 cggaggggca acaatacagg atgtaattcg ccgtagtgaa attcttttac gcccacctcc
      781 aggaaaatca aatatcagtg tgaacaggtt taatggtgat ataccccaaa tggggaaaaa
      841 cgcagcttat gtagccgctc gttttgttcg tcaatatgaa caaaatcaga tattacctta
      901 tcaacgtcag gcattgtggg acattcggca gctctatcag tcaacccgga gttttatttg
      961 gcaggatgat cgattaacca ggaaagaacg ctcacagtat ctgtcagtac tcgcttttga
     1021 gcatataaaa gcgcgtcaga aaatcattaa tagtagtcag caggagaata ctatggcaag
     1081 tgaggatatc cgtaaactta ttaagaaaaa accacgcctg tcaaataacg aaataacagg
     1141 gaaagacgag cggaatatac caccggcagg aaaacgtttt tcaagaatag ttcatacact
     1201 gaaaaaccaa attgaaggtg aagataaaga aaattcatat gcaaagtctg tcagtgcagc
     1261 ggatttatat acaagaaaat cgaaactcag taagaatgtg cattatcttg ataaaaacag
     1321 taacaggaca ttattcgttg atacaggaaa tgccattgct ataagaaaag aaggtatgtc
     1381 accggctgcg ctgggaattg cgcttgaact ggccaaagaa cgttttggca gtacgctgac
     1441 tatcaagggg ggtgaagaat ttcgtaacca ggttattgat attgtcgcca gaaatcaccc
     1501 cgatattcat ttttctgaca aagtgatgaa tcggcaactt gaagaacgac gggcagaaat
     1561 tgccgctgaa cgtgatggga tgacaataga aggcgtttct aaaacgagta cgaataagga
     1621 acaggagaaa gagcatgcac aggcagcaga tactaaaaat caaacaaatg tgaaggagtc
     1681 ctcagagcca cacgaatcac ctgaaagcag gacagtagtt aagctggaag gtaagttaat
     1741 agatcacggc aaagcgcctc ttgataacaa gaagggtgct gaacaaagtt attttgttac
     1801 ggtcaggggc agggacggta aagaaaccac acactgggat agtgatttac aaaacgtaat
     1861 gaaggggaaa cgtcggggaa cagagatatc tctggagttg acaaaatcta atccggttca
     1921 ggtcaaaaca cgcgatcatc aaggtaattt gtctatcagg gaagcggtcc ggcagacctg
     1981 gacattaaac aagatgccgg tgggacagga gcagaaaagg aaacagtcgc tggataaaac
     2041 acagcaaaat caaagctcca caccggccaa aacgcagacg gctgttgacc ttattcttgc
     2101 taatccagta ttacggcaga tggctatgac tgcggaacaa aaggaaagag aatttaccgc
     2161 attgcaggct gataaagaat ctgtcagaga acagactgat caggaaataa gaaatcgtct
     2221 cggtgctgga gatcagagca aggagccgtt agccgtcatt ttgtcagatg atgaactacg
     2281 tggaatggcg ttatcgatgg agcaaaaaat gcgggaaatg gctactccag tagataaatt
     2341 ggatgcagtt aaagcggaaa ttaacagtga gattgcagcg agggttgcgc ggaacagcag
     2401 tattcctgtg cctgtatcag aagttagcgc cactgtgaca caaaaagaaa tccatcggga
     2461 agcagagtcg agaggtaaga aagataaagt acatgaaggt gtgttacttg atcatggccg
     2521 ggcagcatac aaatttaagc ctgatataag taaaccagaa gatgaacgcg ttgatagcta
     2581 ttttgtgaaa ctgaagatgg ataacgggaa aacacgaaca ctatgggggg ttggtctgga
     2641 gagtgttgta gccggttata ataaaggtga acgtctgcgg ctggaggata aaggtacaga
     2701 gaaagttcag tggattgaaa agatgaagga cggaagagaa attgaaaaaa atggcgatcg
     2761 cagggtctgg gactgtagat cgttgactcg tgaatctgaa gttatgaagg gattcagagt
     2821 atcaggtatg ccggagcgtg atgatggtcc tgatgtagcg taacttatac accgcgcctt
     2881 atatccctgc ccttaagggc ggggttttaa aaacaagcag gttcagcaag gcaagttccg
     2941 accgttaacg taatcccaca ttcattcttt gttcagcagt tgaaaccgta agtttctgtt
     3001 caaatgctcc cgcaggtccg tttcgtgagc tgactccttt ttgatcctgg atttgtggag
     3061 ggtgttagtt aagtaattac atgatatatc gacacttgtt ccattcggag caggatcttt
     3121 tctgaataac atcagatctt tatagctgaa ctgtcgatct gtgcgccaac atggatccgc
     3181 cagggtataa ttaagtcgct gatttcaata aagtgtagta tcctctgcaa aatgatcctt
     3241 ttttgatcta tgaggaggct gtatgtctga cgctgtacgt tcggcaactt cctctgaagc
     3301 cagacctaaa cgggcatata gaaaaggtgc accgttaagc gttaatgagc gaaagaggtt
     3361 gtctctcaat cgaaagcggg atacacataa agcgcttaat gttttcatcc gtaatgaatt
     3421 gaaagatatt cttgagcgct tttgccagga taagcatttg accttgggtg aagggatcga
     3481 gttgctaatc caacaggaga aggaacgagc gaaaaatggt gagtagtcac ctttatttga
     3541 tccttttctg gcagagtgat agattacgga cagaaaattc atggctggcc acgccgtaag
     3601 gtggcaggga actggttctg atgtggattt acaggagcca gaaaagcaaa aaccccgata
     3661 atctttctat gtttggcgac tcagaagatt accggggcca tctaaaaacc gcatagccaa
     3721 caattcagct atgcggggag tatagttata tgcccggaaa agttcaatac ttctttctgt
     3781 gctcactcct tctgcgcatt gtaagtgcgg gatggtgtgg ctgatcttca acaaacgtat
     3841 taccgccagg taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc cggaacgctg
     3901 aagttttgcg aaaaactgat ggaaaaggcc gtgggcttca cctcccgttt tgattttgcc
     3961 gcgcatgttg cgtatgcacg ttcgcgtggc ctgcgccggc gtatgccgcc gatgctgcgc
     4021 cgcagggcaa ttgatgcgct gttgcagggg ctatgtttcc actatgaccc gctggccaac
     4081 cgcgtgcagt gctccattac cacgctggcc attgagtgcg gactggcgac ggagtctgag
     4141 gccgggaaac tctccatcac ccgtgccacc cgtgccctga agttcctcgc agagctgggc
     4201 ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc
     4261 acgttcacac tggcgctgtt tgccgccctt gatgtgtctg aggatgcagt ggcagctgcg
     4321 cgccgcagcc gtgtggtatg ggaaaacaaa cagcgcaaaa agcaggggct ggataccctg
     4381 ggtatggatg aactgatagc gaaggcctgg cgttttgtgc gcgagcgttt ccgcagttat
     4441 caggcagagc ttaagtccca cggcatgaaa cgtgcccgtg cgcgccgcga tgcaggcaga
     4501 acccgccagg acattgtcac cctggtgaaa cggcagctga cgcgtgaaat cgcggaaggg
     4561 cgtttccggg gaagcctgga ggctgtgaag cgcgaaatcg accgtcgcgt gaaggaacgc
     4621 atgataatgt cgcggaacaa caactacaca cggctggcca cagcctcacc ctgagtttga
     4681 cctcatctga ataatccggc ctgcgccgga ggcttccgca cgtctgaagg tcggcgacgc
     4741 acaaaaaagc agcgcagcac acaaaaaaca gcctcatcat ctgctttccg gctcgcaatg
     4801 ctcccccggt ttttcacgca aaacacgtct cacagacggg gaattccgct tatccacatt
     4861 taactgcaag ggaattctcc ataaggttac aatcgttcat gtcataaagc gccagccgca
     4921 ggcgttacag gctgcaatgt ttcttttaaa ccccagtact gtatttcctt taaactactt
     4981 aattacattc atttaaaaag aaaacctatt cattgcctgt cctgtggaca gacagatatg
     5041 cacctcccac cgcaagcggc gggcctcgac cggagccact ttagttacaa cacacaaagc
     5101 ccctccctca caactgaaaa gcggccccgt cccggccctc cgggccggaa cagagtcgct
     5161 tttaattatg aatgttgtaa ctacatcatc atcgctgtca gtcctctggc tggaagtccc
     5221 cagtacacgc tcgtaagcgg cccttacggc ccgctaacgc ggagatacgc cccgacttcg
     5281 ggtaaaccct tgtcgggacc actccgaccg cgcacagaag ctctgtcatg actgaaagcg
     5341 gatatggctt agcagggctg gggatgggta aggtgaaatc tgtcaatcag ttccggctta
     5401 cgccgggctt cggcggtttt actccgctgc catatgcaac aacggagtgc cgccttacat
     5461 gcgctaacgc gcataaatcc ccgttcctca gtggaacgaa aactcacgct agctgggaaa
     5521 tatgcacctt gttttgtagt ccattatgga ctacattagc aacaccaata tttgattaat
     5581 gaggtgatga gatggaaaca tatatccgta ctcgtgttga tgatgttctt aaaagccaat
     5641 ttgaaactat cctgcaagat tgcggattat ctgttagcgc tgcattgcgg ttatttgctg
     5701 aaaatgttgt ccgtaatgaa gggttgccat ttgaaattac ccgtaaacca tcagctcgtt
     5761 tgcgtgaatc catccgtcaa accgaagaac tgatggcaca agggcggcca ggttttgaaa
     5821 atgttggtga gctgattgcg agtatgaaca atggggagca ataaacgcgc accgttgcct
     5881 taccggacgg atcgcactaa gaactttgaa aaggcatgga aacgctataa caaaagcgga
     5941 cgctatgata tgcaggcggc tgccgtagca atgaaccttg tgactaatcg taatcctgtt
     6001 ccggctgaat atcgagacca tgaacttact ggagagtggg ctggtttccg agaattgcat
     6061 attgggggcg attttctact ggtttatcgc gtcgatgaaa aaaacgagat tatttatttc
     6121 actgatcttg gtactcatgc tgaactcttt gaataaaaat tgcgtcacgc aatgactgcc
     6181 ctccacgctc acggtatggg gtttggggag caatggaaca gtaaacgccg ttaagaagcc
     6241 aatttaaata gccaaaaatt aactagccaa tttacattgc aggttgtaat ttttaaccac
     6301 cttttttacg atagccaaat tacctatact catcttggcc gcttgcggaa caaaagacgt
     6361 tgttgatctc aacagattga tttacctgtt attgaccatt caattgaacc gcttaaccgc
     6421 cttttgagtt ccactactcc ccaaacccct gatttaactg gagggttgac cggagagaat
     6481 tgaagggaga acgggcagaa gaaaagccgc tgccgcgccc cgtttttatc ggaagtaaat
     6541 attataatta gtaattaaaa caagaaccaa caacaaaagc ggaaacacat ttttaatggg
     6601 tgctgctgcg ttcgcaagcc ccattgtact tacaaaactg aatgcagcca gtaatactga
     6661 aaacatcacg aaaaaacaca tctcaataat attaccagaa tgagatatca tggggaaaaa
     6721 tattccggca gagttaacca gggaagacac aagagttaaa acgacaaaga aattgttaac
     6781 agggaaaaca gtgtcgtttt tagcaattgt gctatcttta tcctttttta aaataataaa
     6841 tttcatttat cccccc
//
LOCUS       DAZKJR010000205         6833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKJR010000205 DAZKJR010000000
VERSION     DAZKJR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.206"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(102..779)
                     /gene="tli1"
                     /locus_tag="ACHOWS_004778"
     CDS             complement(102..779)
                     /gene="tli1"
                     /locus_tag="ACHOWS_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFQ0050246.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMKMCKGVTKSDFSYGYDEDIKEFIRDK
                     TYPYGNW"
     gene            complement(799..2481)
                     /gene="tle1"
                     /locus_tag="ACHOWS_004779"
     CDS             complement(799..2481)
                     /gene="tle1"
                     /locus_tag="ACHOWS_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFQ0050247.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(2478..4997)
                     /locus_tag="ACHOWS_004780"
     CDS             complement(2478..4997)
                     /locus_tag="ACHOWS_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFQ0050248.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            complement(5076..5303)
                     /locus_tag="ACHOWS_004781"
     CDS             complement(5076..5303)
                     /locus_tag="ACHOWS_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050249.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            complement(5300..5941)
                     /locus_tag="ACHOWS_004782"
     CDS             complement(5300..5941)
                     /locus_tag="ACHOWS_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050250.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTRHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(5928..>6833)
                     /locus_tag="ACHOWS_004783"
     CDS             complement(5928..>6833)
                     /locus_tag="ACHOWS_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0050251.1"
                     /translation="SIAQRLRAANTGLTSGNGPQGVFLLVGPSGVGKTETALALADVM
                     YGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVE
                     KAHRDVLNLFYQMFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTES
                     ELHELIRPLLRDHFQPALLARFQTVIYRPLTQSAMRIIVEMKLAQVCERLHCHYGLST
                     SVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTWN
                     DEDGIVIAFDGINSDYPQESIYGQC"
BASE COUNT         1790 a         1829 c         1359 g         1855 t
ORIGIN      
        1 tagtattttt tactgcgtta tcattcagct attgaccgtt tttttacgcc agatgaaaat
       61 tagactttct ttttacatac tcatcattat tctggcttaa gtcaccagtt accgtaagga
      121 taagttttat ctctgataaa ctccttaata tcttcatcat atccatatga gaaatcactt
      181 ttagtaactc ccttacacat cttcatttta tcacccgata ctgtagtaat tttagcatcc
      241 aactgaggta ccaccggatt accgcgatct cccagccaga ctcttacctt cccttcaggt
      301 gccagaccaa taatcatcgt tgaacgataa taggtttcgt tccagcggtt gtctgtgtaa
      361 ggcgtaacca tctgctccca ggtccctttt ttaaaaaaaa tcagagtttc ataactcttt
      421 ttatcaatta ttgaatccca gcaaaaacgt aacatcagag gtaatgcttt ccccttgtta
      481 taatacgact cagtcgcgcc cagatggtca ttccagcgcc ctacactgac ctgactcggt
      541 cttgtggtat caagatactg ataagtcgtc ttataacctt ccccgtccac catcaatgcc
      601 tgagtggcca gcgcaggcag agcctttgga taaataaaga gaaatttcca ttcatcatag
      661 ggtaattttt tataacccca ttcagtctta tccgaatcag catactgtga gctaaccgaa
      721 ttacacccag ttaacaatgc cagtaatggt gcgataaaaa tgattttatc tctggtcata
      781 tattgccacc ctgtggtgtt aaacaccaga catcataact ttagtatttt ttactgcgtt
      841 atcattcaat cgctgctgat catccatgta ccatacagaa cgaatccagc gatcatttgg
      901 tttattcgga aaaacgaacg ccttattacc tttaggacca aagcgatgga taaaaatctc
      961 tcccgttgta ggatccaccc ataaattacc atcagactca atattccagt tggcagaaca
     1021 atgggtgtat tttccgataa tatacaactc ttcctcagta aaagcctgag gctcttgccc
     1081 caaacgtacc gcttttccct gagcaatggc tttgtcagca agaaatatta gttcagatgg
     1141 aagttgtaac tcagtatttg tttgtcgaat tggttcaaac aatacccctg cttcctgagc
     1201 agcatccagc atcacacgca ggcaaacatt agcccagtca ttaggtactg ccatacgctc
     1261 aaaaaatact gcggctccca cctgcttttg tatcacacca gcccgacgtt gattgctgtt
     1321 gttcacacct acggaacgaa ttttggtggt tagttttcca tgaggcaaaa tatgttttaa
     1381 ggctggcaga gtcatcaaca tttttctggc ttgctctgca ttacggtata ctcgcgtatc
     1441 cgtttctctg gtatcgtcac tgacaatttc aaattccggg caactcagaa acagactttc
     1501 attttcctgc agaggtgaac ctaccggatt gtaaccacct ccaatatctg aatgcgcccc
     1561 tggtaaagcc agttcgggcc acataccttt aatactgttc aggctgaaat tatatcggta
     1621 ttcattcatt gcagtaatct ggaaaacttt cttcacgact gaaggacgca attccagctt
     1681 tactcccggg ttgctacgtc cattaatatc gaagaaatta ctgattccac caatagctgc
     1741 gacagtatca aaaagcccca gaaaacggac ttcaccagaa ggtttaccat cataaaaatc
     1801 gcctctgagc cctttagcaa tagctctggc tatagcagga tcctgttcca taacccgatt
     1861 ggcaaagtgc cttgccgccg ctgctccccg gctaaaacca aaaacatcaa actggatttt
     1921 tgcgatacaa aaatcaggat ttaaattgaa ccccataaat tctgataacg cctgggtgat
     1981 tcgttccatt gcctcgtcgg ttttcgtcac cacaccttca aatatatcca gaatactggt
     2041 gcctaatccc atgccaatga ctgaatctgc ttcaccagcc gcagtcccta tcccggaaat
     2101 gtaagtctga atttgtgctt caacctgact ttccaacaaa gtctgatctg ctacgtaaag
     2161 tgaatttagc cagtgaatat tggaataata tcctcgataa ctgccattcg agatactgtt
     2221 cttgttaatt tcttcaagaa agtcctcaca gctttgtgca tctttttgat ttaccccctg
     2281 tctggctgca ctacactcat taaatttcat caggcgagat gctgtattct cccgattgtt
     2341 tccggtacca tcgaaaaata taccaatggt taaagtcaca ccccgcgccg gttttttctt
     2401 tgcaacaaca actttccagt cattatgaat actggtttta tgggtggcat ctgttacgtc
     2461 atatccctga tattttgtca ttctgtttct ccatgaattt ttaccttccc aaactcaact
     2521 tttactactg acggaatgga ggtgttgact ggcgctgttt taccattttc atcggtgatc
     2581 ccttgtagaa tatcaccttc agccgttgtt aatcgataag gcgtcgaagg ttgcggcact
     2641 cccgcttcat cggtaagaat aaaaccgcca ccaaaccctc gcggcatttc aacagacgca
     2701 atatcaagac ttgttggtcc catcttctgc atattcgtgc tttttaacag gatctgtccc
     2761 gggcatccca gctcaatatt tccaccgctt agtttgatat aagctccacc acatgccagt
     2821 atcagttcct gattagcaga aacagtcact ttgccctcag aactttgaat atcaagattc
     2881 tgttgggaac tcattgacaa atcgtcactt tgagcctgaa tatttacccg cccttttgct
     2941 gctaacagtt gcatcccctg gtttctgctc aacaggctaa cagcaccttc tgctacaact
     3001 gtaaatgact gcccggcact aatatcagtg ttatctcccg acattatgcc cacgctttca
     3061 ctacccgaag caattcgtaa cgcccgtgca ctaaccatgc caatcccatc aggagcatgc
     3121 aacagcattc ccggcgcagt gagatctgtt agcgatgaat tcaaccgttg ctgacaactt
     3181 atatctccct gagtagcatt tgcagcagtt gcggccttcg ccatacttcg ggccagagat
     3241 aatgcctgct ccagttggcg gatagcttca ttcatatcca gcatctttcc ttgtgcctga
     3301 ggctgcatat ctgcactgat aaatattcct tttcctcccc ggatacttat ccagtcatca
     3361 gtcctcagct cagcgccttc tcccctgagt ttccgggagg cattaacgtt atgaccaaga
     3421 ttaagttgtg tcttaccgcc aaactcggta ctgatcttga tatgctcctc accgcgtttg
     3481 tcctccatcc gcaacttgtt atttgccggc gtacggataa cattgcgcgt accgttctta
     3541 tcggtaacgt gatcaacatg acgtgaatca tgcagggcat gcgcgatata ggggcgatcc
     3601 ggatcacctt cctcaaaggc aatggccact tccgttccct gaatcagcgg aaaatgaaaa
     3661 ccataagcat caccactgta gggtttggcc agacgtaccg gcatgctttc ctgtcccgga
     3721 tttttatcat cacggtcagc atcaaatttc acccggtaca tacctgatgc atcctgccag
     3781 gcgtagatat ccccttcttt tgcactggtc acacgggccg tcatggtacc ggtaactttc
     3841 ggacgcggca gtaaaggagg gcgccagcag ataacttcac tgtaaggaac gcccttcagt
     3901 acaacctgca gggcatcctt gcgacttgca gaaaaaccga cacaggtaag caacaccgga
     3961 ttgcgcagca gcgcaggcaa cgtcggcgga agactgtcgg tgatagtcag aacctgagca
     4021 ggtgccagcg tggcgtctgt actgttgccg gttatccgca cctgctccgc cagataacgt
     4081 tcatgaccaa ggcgggcata aaaattggcc gtctccggca ccgggtcgat tttatccccc
     4141 cgttccagat gacggaaatg gtagtggtac acctcaccat agttaattcc gtcaccatca
     4201 ccatgcgtca tgtctgccgg ggcggactgc aacagatatt gtgcttcacg atggttataa
     4261 gcctttgtgg tgacactggc ctctgccaca ttgtgcgtca cattcagccc ccacactgaa
     4321 tccgctctgt tatcattcat tccggacgga ctgtttatcg ccagcgtctt atcaaacgtc
     4381 agcgcagcct ggctgtcacc aaaatgcact acttccgtct gcgtgtccgg ttgcagggtg
     4441 aaaaaataaa aaatccccac ttcagccagc aaacgctgaa taaattgcag gtcactttcc
     4501 tgatactggt taatctgttc acgcttcgga tacgtttttt tgagacggaa ctcaaactcc
     4561 cagcctttca ggccgtgctc ctggagaacc tctgtcacca cctccggcac cgatttgtta
     4621 acgaaaaatc ggtgagaacg atgctgcctg tccagtagtt taataaatgg ttccagtatc
     4681 acctggtatt ttgcctggtc ggcggagccc gatatacggc gaaaatctgt aatgacacca
     4741 tgtactactt tctggctcac ctgctcctta agccctcccg tacccattgt aaatgtggct
     4801 gatttacgca gcagcagccc ggcatccagg tcgttgtcag tactggtaaa attgatgatg
     4861 taataatagg tctgactcat ctcctccctg ccgctgaaat gctcaacatc cagtggtgac
     4921 gggcaggagg gaataattaa atggtaacga ttcagggaag cagagatagt ttttgaaagt
     4981 gtatctgtca gactcatatt gttaactcca tgtctgtacc ggtattgaac tgcaattata
     5041 taatgatgac cgttaagctt tattttcaga gagaattact gataatcatc ccattcatcg
     5101 taaatatatg catatgcaaa tcgatacatc agataaccga caaaaaataa cgcaacagag
     5161 agccctcccc acagatattt aaattcagca gatgagagaa aaaagaacca gactgtaaat
     5221 acaatacccg acgcacctaa ccccatacct gcgccacgga taaatcggga aagcattata
     5281 agtaatgttc tttttatctt cataaaccac cgaaaaaata ttcgcatatc cggtcaaact
     5341 cagccgaatt tcggtctgcg acaatacagg ctcgttcaaa cgggcgcaca atatcttcaa
     5401 caagaaagta caaaagatcc aaatccccct gagctttcag cttatcatgc aacaccggat
     5461 ttctgtcttt cagaaaacgg gatgtgtaaa tcgcacgcga tgtctctgcg ccaacagata
     5521 ataacaaccc ggcaaaactc cctgcaaaaa aagcagccag ccctctggat accagaacct
     5581 ctcccagttt aaccccggtc aattcagcaa atgccattct acctgccaga tctcctgaaa
     5641 cgttcttcac aatggagcct gttttttcca tattgatccg ggatacaaag tcttcaagac
     5701 aaatactgat aattttccca ataatatcac ggctgaaaat gcctttttct accagtctgg
     5761 ccattctgaa tttgtcattc tggttttccc gcctatgcct ggtatctata aaatcatatc
     5821 ccatataacc gagactgact ggcaaagata tgagtccctt cccgacggcc cccagaaacc
     5881 catccccatt aatatttacg gcctccagca tatcccgggc aatttcttta gcactgtcca
     5941 tatatgcttt cctgcggata atcgctattg attccatcaa acgctatcac aataccatcc
     6001 tcatcattcc acgtcagaga caggtacgtc ggatgctgtc tggcagcaag atagctgagc
     6061 aactgccggc tcagtaccgg cagcagttgc tggttcagca gactctccac attccgggca
     6121 cccgtatccg gcagcaggca ggcagatgtc agggcatcgt acacacgttc atcaaccgat
     6181 gtgctcagtc cataatggca gtgcaggcgt tcacagacct gtgcaagctt catctccaca
     6241 atgatgcgca tggcagactg cgtcagcgga cggtaaatca cggtctggaa acgggcaagc
     6301 agtgctggct ggaagtggtc gcgcagcagt ggacggataa gctcatgcag ctccgattcc
     6361 gttgtctccg gcttttcgct cagttgctgc atcagaaggt cactgcccag attggaggtc
     6421 atcagaatga ccgtattacg gaagtcaatt tcacgcccct caccgtcgcg cataaagccc
     6481 cggtcaaaca tctggtagaa cagattcagc acatcccggt gggccttttc cacttcatcc
     6541 agcagcacca cgctgtaagg gcgtttacgc acagcctccg tcagaatacc gccctgaccg
     6601 tatcccacgt acccgggcgg tgacccctta agctgggaaa ccgtatgggg ctcctggtat
     6661 tccgacagat tgatggtaat cagtgatttt tcgccgccgt acatcacatc tgccagcgcc
     6721 agtgccgttt cggtttttcc cacaccactg ggaccgacca gcaggaacac gccctggggg
     6781 ccgttaccgg atgtcagacc cgtattcgcc gcacgcagac gctgcgcaat gga
//
LOCUS       DAZKJR010000206         6816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKJR010000206 DAZKJR010000000
VERSION     DAZKJR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.207"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(379..1269)
                     /gene="ybcH"
                     /locus_tag="ACHOWS_004784"
     CDS             complement(379..1269)
                     /gene="ybcH"
                     /locus_tag="ACHOWS_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFQ0050252.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
     gene            complement(1270..4242)
                     /gene="nfrA"
                     /locus_tag="ACHOWS_004785"
     CDS             complement(1270..4242)
                     /gene="nfrA"
                     /locus_tag="ACHOWS_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFQ0050253.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHGMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRH
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPRKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGIEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4229..6466)
                     /gene="nrfB"
                     /locus_tag="ACHOWS_004786"
     CDS             complement(4229..6466)
                     /gene="nrfB"
                     /locus_tag="ACHOWS_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFQ0050254.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNPEQVAQLES
                     ENEGE"
     gene            complement(6664..>6816)
                     /locus_tag="ACHOWS_004787"
     CDS             complement(6664..>6816)
                     /locus_tag="ACHOWS_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFQ0050255.1"
                     /translation="AAELFSGLRHIAINILTNDKVFKAGLRRKLRKAAMDRNYLASVL
                     AGSGLS"
BASE COUNT         1608 a         1780 c         1779 g         1649 t
ORIGIN      
        1 tttttagtat aaaaaagagg aacaaaactg agacacataa ggcctcgcaa tggcttgcaa
       61 ggttttacat attttgaggt ggtggaacgt gtgaacgcag agatggcgcg gtaatttgtt
      121 gatttaaaat gtcgttcctg gagtttctaa gtcgtgggct acaggttcga atcctgcagg
      181 gcgagccatt tcctcgccat ttatttgttc ccttttacaa attacaccat cactttgttg
      241 tcgcggtttt gctaaataat tggaacacgt cagacgaata aaatgaaaac agaaattctg
      301 ctaaacaatt catcttgcca ggattttgat taggaaagtg aaaaggaaag gagcaagcta
      361 agttgctccc tttaatcatt aatactcaag gatactctgc gcgacgggca aatagcgcag
      421 agagaaatag agcgtgtctt taccgcaaga ggaacgtttc gctaacagcg aggcgatctc
      481 tgcatccggt ttcggtttcg ctgtaaacgt tttaccttta ccagcgacaa acagttcata
      541 aacaatgccg cttgctgggg cggaactttg gcaatcggcg ctggcctgta gataacgttc
      601 acgctgttca gcggtcagtt tatccacgcc gctgccatcc tgtacccaga cattaacacc
      661 ggttgcttta acttgttcca gcagttggcg atagccatct ggcgacatgt ttccggcgaa
      721 aaaactactg atataaaccg gttttgctga aacatcgcta atcaaccgct gcgcgttgtt
      781 taaccatgtt agcaaaggtt gacgggcggc ttcgctgcgc cagttcaggt cgtcaatttc
      841 cgcgctgatg taccagccat ccggcgttac gccaggcacg gcgctccata atctggcttg
      901 ctggagatcg gcagccagca ggcgattaag atagctttcc agcgctgcgg aggactgttt
      961 ctggtgtata aaaaattccg gatcggcgtt cagcccgaca atcagcttca ggccagcctg
     1021 ttgcgcagct gcggcacgct taaacaataa cgcgcgctgt tctggctggg tgaatgcatc
     1081 gccgtaacgg gtccattgca aaaccagggt atcgaagcct tgcaaacgta actgactcat
     1141 cagcccctgc cactgggtat cggtaacctg actatcacgg ttttgtggtt gccagataat
     1201 acctttcatc gcaaaggaaa aagggctgac caaaagcagt gtcaacaata cgaaaatgaa
     1261 cttacgcatt taccagtgca ctccaatggt gagaaacgcg ttgttgcgct ctccgttacg
     1321 ttgattaatc gccttaaagg tatgttgata ttcgatgccg agactgactt tgtgcggcca
     1381 ggcgtcgtag tgcgtctcgc cggtccagat attccagcgg accccgacac cgccaagctg
     1441 cgcgccctga gtgcctttat cacgataacc gttgtcctga acgtgagcat aaggctcaat
     1501 agtctgcccg tttgccacct tctgatgcca gctgactcga taatctgccg tccacgcctg
     1561 aatatcctgg cggatatatt gcgccgcatc gaggtacagg ttttgggcaa accagcctga
     1621 accgttcggg tgccattcgt cgctgtattt gccgccatta aagaatgagg cgctggcgcg
     1681 cagcatggta tcggatgcgc cattttgtcc gttcagcggc aactgctgtt cgacggcgag
     1741 gaaaaagatc tgatcgcgca gcggcttcca gcgcagaccg gtgccggaca tcggattttt
     1801 caccggcatc atcaccccgt tttcaccggt atcggcaaag acgcggctat aaactgagag
     1861 caggtcgcct tccagcagca tattgcgtcc gatgcggtac tcggcttcca gttgcccgta
     1921 gctacgatag cttttccttg gcgctgcgcc gccgacatta ttgttagcgg tactcattgc
     1981 gccggaacgc aagccaatag aagaatcgaa actgaacgtc cagtggcgac cgacctcctc
     2041 atgcagacgg cggaaattga agcgttgctg attttgctct ggggtcagtg gggttatcag
     2101 cgcctgatta tcaatgtcat caatcaccag tcgggcgtag tgctgcgtcg caggcatgtc
     2161 atccagacgc tggttcacgt aggccagttg tcggataagt gccggatcgt ccggtagccc
     2221 tttatgcgcc tgttcgagca tttcccgcga ctgtgcgata tcaccgctat cccacaaggc
     2281 gtaaccgagc gctgcctggg tgttgctatt attcggttcc agttccagag cggcgcgcaa
     2341 atcactcacc gccgccggga cattatgacg ttggcgataa attgtcgccc gcgcaacgta
     2401 agcgttggca gaaggcgcaa tattgattga gcgcgtgaga tcgctcagtg cgagttccgg
     2461 ctgaccagga atgtaacgtt gcgcatgcag ccaccagtag agggcattgt ttcccagccc
     2521 acgttgttct gcctgctgaa gccagcgatc gcgagccgca ccatttcctg ccgcctgggc
     2581 ggtattggca gcagcaagca gatcctcatt gctcatgccg tgaagactga ttttctgcca
     2641 ggccgccagt gcggtggcgt agtcctcaac ctgatacgcc tgataggcta ccgcacgatg
     2701 ttgccaggcg ttcggttgtc gttgttcggc ctgaagccat gcatacaacg ccacaccggg
     2761 tagcgtgtcc cgataacact ttgccagacg gttccaggcg gcagcatcgt aggaaggcga
     2821 catatcgccc agcaagcgaa ctattgccgg gcaattatct gcaatacccg gcaactgact
     2881 ttgccactga cgttgctccg ccagcggtaa gggtttcgat aaaatcgcca ccttcgccgg
     2941 agttgccagg taaggatgac tttccagcag agacgccagt cgcgccatta aagtctggct
     3001 gacacgcgca tcgccctgga aaggatagcg ttgcagcaat aaatcggcag cttcacgtga
     3061 ctgctcgttc tgcatcagtt gccaggtcag ttgatccagg cgggtaagat ttgccggttc
     3121 ttgctgatac agcaatcgtg ccagacgcag agcttcagcc ttgttacggg tcgccacgct
     3181 aacggtataa cgctcctcaa gcatttcatt ggcggggagg gtggcgagca gtttttgcgc
     3241 tgcgtcgtac tgaccttctt ttaacagcac cggtagcgtc gcgccaacaa catactggcg
     3301 gttgtcggca aactgtaccg tataattcgc caacgcctga acggggttgc cgctgtattt
     3361 agataacaga tagagccaac ttttctcttg tgcgtccgtg gtaaatagcg gcgtattttc
     3421 aatgagataa tgctggaggc gtgctttttc gccacgataa gccagcgcgg tcgcgtaagt
     3481 aatatatgac tgaggatcgg tgaagatccc ctgtgattgc agcgccagga tccgatcgtc
     3541 cagctgcccg gcaagaagca catcaaacca ctgacggcgt tctgccgcgc ttaatgtgtt
     3601 ctgctggcgt gcttcattgt atagcgtatc tgcctgggac cattgtttca ggtagattgc
     3661 ccgttgcagc agatcggttc gcagcgtttt tccttccggc gatgcagcaa acgtcgcatc
     3721 gttcagttgc gctctggcga caggtaattg tgccagccgc agggcattct gcccgacttc
     3781 actgcgacaa cgcagggtcg gcgcagcatc gcacgctttt tgctgggcaa gcagttcttc
     3841 aacagtcgta acgcttttca cttcaactgg aatagccgcc agactgcgct caagtcgggc
     3901 atctcctggg tgacgtttca gttgatcctc aagcaacagc cgcgcccggt catcatgacc
     3961 aaaatggcga taggcttccg caaggtataa agtcagcgga atattatccg gcacctgttg
     4021 gtgtatatat tcaaattcgc ggatggcggt tgcttcgtcg ttatttttct gtgccttcag
     4081 cgccttatcg agacggggat aaataacaaa atggcgataa tcgctcagcc ccagctcttc
     4141 tgcgctggtg ccgatattgt ctgcgagtgc gctggtgctc aataaagacg tcagcagtaa
     4201 accagaccat ccgatgacgc gattaaggtt attctccttc attttcggac tccagttgcg
     4261 caacctgttc tgggtttaaa cctgctttga gtaatagtga ttgcatcgaa acttgtaatt
     4321 cgcgttgaat tgtcaggacg cgatccaacg tttcctggct gataacgcct tcggtgacca
     4381 aaaacttgcc gagcggcaga gaactgcgtt catggcgcaa taacaacacg ttaattgctg
     4441 aacgattaat atgaccgagc gtggtcagta tttcggcgaa caggaactga tgcggcacat
     4501 attgccgcca gatttcaccg gcctgctgtt ccgtgagcca ctgatgctga accgcattgt
     4561 acaacattgc ccgcgggtcg tgaccgcgtc ggcgtgcata ccagtgacgt aaccccgtga
     4621 caatttgtcc gcgcagaaca atgacgtaac gcactttgcg tccgacttta cgcgtcaggg
     4681 ctgccagcga aaccgggtca ataccatctt cactgccgac aattaactca tcattttcca
     4741 gacgcagcgg cagcactgca taatgcagcg ccacggaagc cggcatttcg gcaatcagtg
     4801 atgaagggat ctgccaggcg tcgatcgact cccacgccac gccgttttgc tctgccagcg
     4861 cctgtgccag ctgttcggcg ctaatcagcc cctgcatcag cattgaaccg cccaggcgta
     4921 gaccttcgac gcgattacgc agtgctgtat cgagttgttc ttcagtgatg acctgatttt
     4981 ccagcaggat ttggcctaac gggcgcaacg agcgggtatc gccagtcacg ctggggaagt
     5041 catgcgttgt tttatcccac gcgacgcgac gtggatcgcc gtgttgaagt acctgtttta
     5101 acgcgcgcca gttggccatg aagttaatca ggttgcccca gaaaagacgc aggacagata
     5161 gcagcccctg cgtcagaccg tagtagccag tgacaaaaat cacccgctgc acgatgcggt
     5221 taaccatcaa accaaagttt agccacagca gggtcattaa ccatgcgctg ccgctgaaaa
     5281 tagaaaggaa atgccaggca tcgggccaca aactttcata cgccagtaac agcaaaagct
     5341 ggatcatcac cagcatcgcg aggaagctga caaagttact gattgcccct ttgcggtcgc
     5401 gccagagaaa gtagttcagc gtcaggctgg aggtccattt gtgggtttta aagccctgga
     5461 aaacaatgcc gatgatccag cgagattttt gtcgaaccgc agtcgaaaag gtatcgggga
     5521 aatattcgcg cacgcagatc atgtttgacg tccgcgcgtg ctgtaaaaat ttacgctgct
     5581 cgcgttcttt ggcttcgtcc accaccggaa aacggacaaa aatttccgtc ataccttttt
     5641 ctttcaggcg gaaaccaatg tcgtaatctt cagtcagact ctgcacgtcg aaagcaatac
     5701 cgtcaccgtc agctaacagt gcggtcacgg cgcggcggct gaaacaggtg ccgacgcctg
     5761 cgctgggcac ctgtccggcg agggcttcac gcaccggaac atctttgcca tgcagttctg
     5821 aaaactcatc aatgtaggtc atgctggtga agtgcgtcca ttcgcgttcg aacggataca
     5881 ctgggatctg aatcagatct ttacgctcga ccagatagtt gaacagacgc aattccatcg
     5941 gtgaaatcac atcttcggcg tcatgcagaa taaaaccagc aaaagcgaaa ttggcgctac
     6001 gttcaaattg ggtgatggcg tccagcacgt tgttcagaca gtcggctttg ctggtggggc
     6061 caggacgcgc gcagactacc ttatgcacgt tcgggaagcg agcgcacact tcgtcaacat
     6121 cacgctgagt atcggggtcg ttggggtagg tgccaacaaa gatatgatag ttttcgtagt
     6181 cgagcgtggt cgccgccagc tcggccatat tgccgatgac gcccgtttca ttccacgccg
     6241 gaaccataat cgctaacggt ttttcatctg gtttatacag ttcgcggtaa ctcattcgcg
     6301 ggtagcggcg ataaacactc aacttgcgtt taatgcggcg tacccagtag acgacatcaa
     6361 taaaaaaatc gtccagcccg ctgatgaaca tgatgaccgc taacgttatc gcgattactt
     6421 ttaagccgta gagccaggta gcaaaaacat caagaagcca gtccacacaa aaaccttaca
     6481 ttaacgctgg ttatgtttag ggtggcgtat attaaggttt tttatgaatt gtgacagctt
     6541 tttaccatta ataggtatga ctattgcggc acgttatttt tacactggtt ataaaagttg
     6601 ccgtttgcgt agaatgtaga caaaagcgtg gtgtcaagga ggggaggtta acgcagggaa
     6661 agattacgaa agcccgctcc cagcaaggac tgacgcgaga tagtttctgt ccatggctgc
     6721 ttttcgcaac ttacgtctta accctgcctt gaatacctta tcattcgtca aaatattaat
     6781 agcgatgtgc cgtagccctg aaaataattc tgctgc
//
LOCUS       DAZKJR010000207         6778 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKJR010000207 DAZKJR010000000
VERSION     DAZKJR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.208"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..867
                     /locus_tag="ACHOWS_004788"
     CDS             <1..867
                     /locus_tag="ACHOWS_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFQ0050256.1"
                     /translation="HLGHDNNGGIARGATPESSGSYGFVRLEGDLLITEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKA
                     SSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMNFGKGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSS
                     RGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAE
                     GYNGQATLNVTF"
     gene            938..1171
                     /locus_tag="ACHOWS_004789"
     CDS             938..1171
                     /locus_tag="ACHOWS_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFQ0050257.1"
                     /translation="MPGCTARLLPEGPFSRGQAVAVTTAYRNVFTEDDQGTYFRLVIR
                     NAEGQLRWRCWNFESDAGKQLNVWLSSEGLLRQ"
     gene            1261..2079
                     /locus_tag="ACHOWS_004790"
     CDS             1261..2079
                     /locus_tag="ACHOWS_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFQ0050258.1"
                     /translation="MRLASRFGYANQIRRDRPLAREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTHAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            2134..2619
                     /locus_tag="ACHOWS_004791"
     CDS             2134..2619
                     /locus_tag="ACHOWS_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFQ0050259.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIVACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            2635..3111
                     /locus_tag="ACHOWS_004792"
     CDS             2635..3111
                     /locus_tag="ACHOWS_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFQ0050260.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3174..3395
                     /locus_tag="ACHOWS_004793"
     CDS             3174..3395
                     /locus_tag="ACHOWS_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFQ0050261.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            3558..3932
                     /locus_tag="ACHOWS_004794"
     CDS             3558..3932
                     /locus_tag="ACHOWS_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFQ0050262.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            3979..4356
                     /locus_tag="ACHOWS_004795"
     CDS             3979..4356
                     /locus_tag="ACHOWS_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFQ0050263.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            4353..4841
                     /locus_tag="ACHOWS_004796"
     CDS             4353..4841
                     /locus_tag="ACHOWS_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFQ0050264.1"
                     /translation="MKLTLTLEADNVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDCETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            4853..5050
                     /locus_tag="ACHOWS_004797"
     CDS             4853..5050
                     /locus_tag="ACHOWS_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFQ0050265.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLHQNR"
     gene            5135..5980
                     /locus_tag="ACHOWS_004798"
     CDS             5135..5980
                     /locus_tag="ACHOWS_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFQ0050266.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDWWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKECYEDEMFSIRYFKKGSAHITFKNQELVDRLNDIIARHYPR
                     MLPSL"
     gene            6573..>6778
                     /locus_tag="ACHOWS_004799"
     CDS             6573..>6778
                     /locus_tag="ACHOWS_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050267.1"
                     /translation="MGHGTCKAAKSARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLLQNNNVIYLFEN"
BASE COUNT         1673 a         1823 c         1699 g         1583 t
ORIGIN      
        1 catctcggtc acgataacaa cggcggtatt gcccgtgggg ccacgccgga aagcagcggc
       61 agctatggct tcgtccgtct ggagggtgac ctgctcataa cagaggtcgc cggtatgtca
      121 ctgacgactg gggtgtatgg tgctgcaggc cattcttccg ttgatgttaa ggatgatgac
      181 ggctcccgcg ccggcacggt ccgggatgat gccggcagcc tgggcggata tatgaacctg
      241 acacacacct cctccggcct gtgggctgac atagtggcac agggaacccg ccacagtatg
      301 aaagcgtcat cgggcaataa cgacttccgc gcacggggct ggggctggct gggctcactg
      361 gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccaca actgcagtac
      421 acctggcagg gactttccct ggatgacggc caggataacg ccggttatgt gaagttcggg
      481 catggcagtg cacaacatgt gcgtgccggc ttccgtctgg gcagccacaa cgatatgaac
      541 tttggtaaag gcacctcatc ccgtgccccc ctgcgtgaca gtgcaaaaca cagtgtgcgt
      601 gaactgccgg tgaactggtg ggtacagcct tccgttatcc gcaccttcag ctcccggggt
      661 gatatgcgtg tggggacctc caccgcaggc agcgggatga cgttctcacc gtcccagaat
      721 ggcacgtcac tggacctgca ggccggactg gaagcccgtg tccgggaaaa tatcaccctg
      781 ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg ctataacggt
      841 caggccacgc tgaatgtgac tttctgataa tcaggtcact ccccttcagg ccccggtcat
      901 catgaccggg gctttttact ttcagggagc aacagatatg ccgggttgta cagcacgttt
      961 attgcccgaa ggccctttct cacgcggaca ggctgttgcc gtcacaacag cttaccgcaa
     1021 tgtgtttact gaagatgacc agggaacata tttccggctg gttatccgta atgccgaagg
     1081 gcagctacgc tggcggtgct ggaattttga gtctgacgcc ggaaaacagc ttaatgtgtg
     1141 gctctccagt gaagggcttc tcaggcaata aacgtcttca tttcatccat caggccgcat
     1201 cttctccggg agacgcggcc ttttcattta taccgccaac tcattcataa ggagcaaatc
     1261 atgcgattag caagtcgttt tggttatgcg aaccagatac gccgtgaccg tccactggca
     1321 cgcgaagaac tgatgtacca cgtcccgagt atttttggag aagaccggca cacctcccgc
     1381 agtgaacggt atgcgtacat tcccaccatc accgtcctgg aaaatctgca gcgggaaggc
     1441 tttcagccgt tcttcgcctg ccagacccgt gtgcgcgacc agagccgccg ggaatatacc
     1501 aaacacatgc tgcgtctgcg gcgggccgga cagataaccg gtcagcatgt gcctgaaatt
     1561 attctgctca actcccatga cggtacctcc agctaccaga tgttacccgg atattttcgt
     1621 gccatttgta ccaatggcct ggtctgcggt cagtcgctgg gagaagtccg ggtgccacac
     1681 cggggaaacg tggtggacag ggtcatagaa ggcgcttacg aagtggtggg cgtgtttgac
     1741 cggattgagg aaaagcgtga tgccatgcag tcgctggtcc tgccgccacc ggcacgccag
     1801 gcgctggcac aggcggcact gacttaccgt tatggtgacg aacatcagcc cgtcaccacc
     1861 gccgacattc tgacgccacg acgccgggag gattacggta aggacctgtg gagtgcttat
     1921 cagaccattc aggagaatat gctgaaaggt ggaatttcag gtcgcagcgc taaaggaaaa
     1981 cgtatccata cccatgccat tcacagcatt gacactgaca ttaagctcaa ccgcgcattg
     2041 tgggtgatgg cggaaacgct gctggagagc ctgcgctgat tcagccatta ccctgaaaac
     2101 cacgcattta tttgtccttt aaggagtcat ttcatgaaaa ccatttcaca taattccacc
     2161 acatcttctg tttccgtaac cgctgcatca gggaatgacc agtctcagct ggttgccact
     2221 cccgtccctg atgaacagcg catcagcttc tggccgcagc attttggcct cattccacag
     2281 tgggtgaccc tggagccccg tatcttcggc tggatggacc gtctttgcga agactactgc
     2341 ggtggtatct ggaatctgta cacactgaac aacggtggcg catttatggc ccccgaacca
     2401 gatgacgatg atggcgaaaa ctgggtactg ttcaatgcca tgaacggtaa ccgcgctgaa
     2461 atgagcccgg aagccgccgg cattgtcgcc tgtctgatga cgtacagtca tcatgcctgt
     2521 cgtacggagt gttatgccat gacggtccat tattaccggt tgcgggatta cgccctgcag
     2581 catccggaat gcagcgccat tatgcgcatc atcgactgaa tggagagaag cacaatgcaa
     2641 caactttcct ttctgcccgg agagatgacg cccggcgagc gcagtctcat tctgcgggcc
     2701 ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg ccttcacctc cacccgtgcg
     2761 gcacgggaat ggctgattct gaacatggcg ggactggagc gtgaagaatt ccgggtgctg
     2821 tatctgaata accagaatca gttgattgcc ggtgaaaccc tcttcaccgg caccatcaac
     2881 cgcacggaag tccatccccg ggaagtgatt aaacgcgccc tgtaccacaa tgccgctgcc
     2941 gtggtgctgg cgcacaatca cccgtccggt gaagtcacac ccagcaaggc agaccgcctt
     3001 atcacggaac gtctggtaca ggcactgggc ctggtggata tccgggtgcc ggaccatctg
     3061 atagtcggtg gcaaccaggt tttctccttt gccgaacatg gtctgcttta acccgtcaca
     3121 accacatcac gcttgttttc acttttatct tctgtcttca gaggtatcac actatgaaaa
     3181 tcatcacccg tggtgaagcc atgcgtattc accaacaaca tccggcatcc cgtctttttc
     3241 ctttctgtac cggcaaatac cgctggcacg gcagcactga agcgtatacc ggtcgtgaag
     3301 tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg ccataaggac agttttggcc
     3361 cgtatgtccg gctgatgagc gtcaccctga actgatgttc cgtcacccgg aaaacgagta
     3421 cgaccagatg gactcagacg ccgctgtgga tgaggccctg ctcatcaacg aatacacctt
     3481 taccgaagca ggtcagcgcg ccggctgacc tgtcatcgtt aaaccattct taaccacttt
     3541 tcagagagga ttttatcgtg tcagacaaac tctccgggat aactcatccc gacgacaatc
     3601 acgaccgccc ctggtggggg cttccctgca cagtgaggcc ctgttttggt gcccgtctgg
     3661 tgcaggaggg taaccgcctg cattaccttg ccgaccgcgc cggtatcaga ggccgcttca
     3721 gcgacgcaga tgcataccat ctggaccagg cctttccgct gctgatgaaa caactggagc
     3781 tcatgctcac cagcggtgaa ctgaatcccc gaaatcagca taccgtcacg ctgtatgcaa
     3841 aagggctgac ctgcgaagcc gacaccctcg gctcctgtgg ttacgtttat ctggctgttt
     3901 atccggcaca ggcacccgca accacctcat aaccccgaag ctctcacctt tagcactacc
     3961 agttacagag aacttaagat gaaaacatta tccgacacac atgtacggga ggtatctcgc
     4021 tgcccgtctc ccgtcaccat ctggcagaca ctgctcatcc gactgctgga ccagcattat
     4081 ggcctcacac tgaatgacac accgtttgcc gatgaacgtg tgattgagca gcatattgaa
     4141 gctggcattt cactgtgcga tgcagtgaac tttcttgttg aaaaatacgc actggtgcgt
     4201 accgaccagc cggaattcag tgcctgtacc cgttctcagt taataaacag catcgatatc
     4261 ctccgggctc gcagggcgac cggcctgatg acccgcgaca attacagaac ggtaaataac
     4321 attacccagg ggaagcatcc ggaggcgaaa caatgaaact gactctgacg ctggaagcag
     4381 acaacgttaa cgtgcaggcg ctgaacatgg gacgaattgt cgttgacgtc gatggtgtta
     4441 atctcgctga actgattaac gtggtctgcg acaatggcca ctcccttcgt gttgttgatg
     4501 aatctgaccg ggtctcaaca gactgcacgc caccatttgc tgcccttacc ggcatacgct
     4561 gcagtaccgc acatatcacg gaaacggaca acgcctggct gtactcgctg tcacaccaga
     4621 ctaatgacga ctgcgaaaca gaatggattc attttacagg cagcggatat ctgttacgta
     4681 ccgatgcgtg gtcatacccg gttctgcggc ttaagcgcct gggcctgtca aaaacgttcc
     4741 gtcgtctggt tgtcacactt acccgacgtt atggcgtcag tctcattcat ctggatgcca
     4801 gcgctgaatg cctgccgggt ttacccactt tcaactggta accaggaaca acatgaaatc
     4861 attaaccacg gaaacagcac tggatattct gattgcgtgg ctgcaggaca atatcgactg
     4921 cgaatcggga attatctttg acaacgatga ggataaaacg gattcggcag cactgctgcc
     4981 ctgcattgaa caggcgaggg aggatatcca taccctgcgc caggtgcagc ttctgcacca
     5041 gaaccggtga gtcttactca ccagacttca ttccactgac gccagcctga acagggctgg
     5101 cgttttcatt tatctgcgaa aaggaatatc gattatgtct gcaatcacag tctcccgtcc
     5161 ggaagtggtc accgggcata cggacgttat ctgctccacc tcagtcagtc acattctggc
     5221 tgtgcgaaag actacactgc tgcaaatcga cacacttatc cggcaactgg ctgaaatctc
     5281 ggtactgaca gaaagtattg gaggtaaaac tgctctggac tgggccatga aacaggattt
     5341 tcgctgcggc tgctggctga tggagaaacc tgaaaccgca atgaaagcta tcacacgcaa
     5401 tctcgatcgt gaaatctggc gtgacctgat gcaacgttcc gggatgcttt ccttaatgga
     5461 tgcacaggcc cgtgatacat ggtaccggtc actggaatat gataattttc cggaaatcag
     5521 cgaggcgaat atcctgagca catttgaaca actgcaccag aataaggatg aggtgtttga
     5581 gcgaggggtg atcaacgtct tcaggggact gagctggaat tacaaaacca attgcccctg
     5641 caaatttggc agtaaaatta tcgtcaacaa cctggtgagg tgggactggt gggggtttca
     5701 tcttatcacc gggcaacagg cagatcgact tgccgatctg gaaagaatgc tacacctgtt
     5761 cagcggcaaa ccgatccccg acaaccggga aaacatcacc cttcgtctgg atgatcacat
     5821 ccagtctgtt cagggtaaag agtgctatga agatgagatg ttcagcatca gatactttaa
     5881 gaagggctcc gcgcacatca cgttcaaaaa tcaagaactg gttgacaggc tgaatgacat
     5941 tattgcaaga cactatccga gaatgttacc ttcattataa agttctaaga atgaattcaa
     6001 ttttgcattt tagcttcatt ccctcacgca aaattatttg ggggttctta tgggggttac
     6061 tcatttatcc aacacaaatt aatcatttaa aattaatata ttaatactca tattcgactc
     6121 ctgtgatcgc caaaatgcct cttcagtgat ccccatcctg cttatttctg agcaacctga
     6181 ccctgctgat gtatctggta gtatgacaga gtgattcatt tttcttgccg agtgtacgaa
     6241 aacgtcttcc ttgccaccga ctatcaaccg tcactcaaaa aaacctctct ttttttcaag
     6301 ctatcaagaa taagttgcat tttatctgca ccggacacca gccatccata tatggctttt
     6361 acctgctaca aagatgaaga agtgcataaa tgtgatgtta aaacaatcaa agaaaaatta
     6421 catgtgcaat aatttcacta taacattata atccatggtg aaaataacac tgcacaagga
     6481 ttgtcatcat caaactcatc ggaactattt tttctgtcgt cattggtgta atactgattg
     6541 taagtatcct ttcaggagat tatttttgcc gtgtggggca tggaacttgt aaagcagcca
     6601 aaagcgctag gcatttaatt gaaagtaaag agaaaaacag aagtagtcgc gtcaaagtct
     6661 ttagcacgca tattaatttg agtcatccag cggcatcctg gctagcaact caaacttgcg
     6721 ctaacaatat attcaatcta cttcaaaaca ataatgtcat ttatttattc gaaaacga
//
LOCUS       DAZKJR010000208         6716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKJR010000208 DAZKJR010000000
VERSION     DAZKJR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.209"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..879
                     /gene="gadW"
                     /locus_tag="ACHOWS_004800"
     CDS             151..879
                     /gene="gadW"
                     /locus_tag="ACHOWS_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFQ0050268.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1242..4355)
                     /gene="mdtF"
                     /locus_tag="ACHOWS_004801"
     CDS             complement(1242..4355)
                     /gene="mdtF"
                     /locus_tag="ACHOWS_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFQ0050269.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGDHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4380..5489)
                     /gene="mdtE"
                     /locus_tag="ACHOWS_004802"
     CDS             complement(4380..5489)
                     /gene="mdtE"
                     /locus_tag="ACHOWS_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFQ0050270.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5597..5875
                     /locus_tag="ACHOWS_004803"
     CDS             5597..5875
                     /locus_tag="ACHOWS_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050271.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5876..6403)
                     /gene="gadE"
                     /locus_tag="ACHOWS_004804"
     CDS             complement(5876..6403)
                     /gene="gadE"
                     /locus_tag="ACHOWS_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFQ0050272.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNNPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1698 a         1623 c         1621 g         1774 t
ORIGIN      
        1 attatcaatt aactgttatg caaaactact ttgtggataa aatttggtcc taccaaatat
       61 ggcagttttt gcacacagaa acagtctggc gtcatttcat tagtatactg acattgaaat
      121 aatcgcagta atgaaatata agggatagtc atggctcatg tctgctcggt gatcctcgtt
      181 cgtcgttcat tcgatattca tcatgaacag caaaaaatat cgttgcataa cgagagtatc
      241 ctgctgctgg ataaaaattt ggcagacgat tttgcgtttt gttcactgga tacgcgacgg
      301 ctggatatcg aagagctgac agtttgccat tacttacaaa atattcgtca gttgccacgc
      361 aatttaggat tgcatagcaa agaccgtttg ttaattaacc agtcaccccc catacagctg
      421 gtgacggcga tttttgatag tttcaatgac ccccgggtca attcgccgat actgagcaaa
      481 atgctctatc tttcctgttt atcaatgttt tctcataaga aagaactgat ccccttactt
      541 ttcaatagta tcagtactgt ttcaggaaaa gttgaacgcc ttattagctt tgatatcgct
      601 aaacgttggt atctacgcga tatcgcagaa agaatgtaca ccagcgagag tctcatcaaa
      661 aaaaagttgc aggatgaaaa tacctgtttc agtaaaatat tactcgcctc caggatgtcg
      721 atggccagac gattactcga gttacgtcaa atacctctgc atactattgc ggaaaaatgt
      781 ggctatagca gtacgtcata ctttataaat acatttagac aatattatgg tgtaacgcca
      841 catcagtttt cgcaacattc gccgggtacc ttttcctgac atatttcgca tttgaatatt
      901 ggtcaggatc tcacgtctgc ttcatgtgaa actctttcct gatgatttct gccgggctac
      961 cggctagttc tctttcgcag tttgccaggc ggtataggta ttttcaggag gaaaagactc
     1021 ggcatgtttg ggattattaa gctaacaatt catacgatta cgggaatgtg ggtcagtatt
     1081 gtgctgttca aattgatgac gaatggctgg agtggtttct actttcagtg ctgtgtacta
     1141 agcctggtat tcctgacggt tagctggttg ttgagtggtg aatggttagc aggaaagagt
     1201 aaggctgaac cctcacgttc aaccttactc tcatttacac gttacgcttt tttaaagcgg
     1261 gcaaagagat gttccactac aacgaaaaag accggaacga agtaaattgc cagcactgtt
     1321 gcggcaaaca tcccgcccat tacgccggta cctaccgcgt tttgcgcgcc agagccggca
     1381 ccatgactga taaccagcgg cagcacgccg agaataaagg ccagagaagt catcaggatt
     1441 gggcgtaaac gcatacgcgc tgcttcgatg attgcctcta ccggcgtttt cccttctttc
     1501 tgcatcatct caacggcaaa ttcgacaatc aggatggcgt tcttggcgga aagcccgatg
     1561 gtggtcagca aaccaacctg gaagtaaacg tcattactta agccgcgcag atcggtggcc
     1621 agtaatgcgc caacgacgcc taacggaaca accaacatca ccgagaacgg aattgaccag
     1681 ctttcataaa gtgcggcgag ggcgaggaac accacgacca gtgaaatggc atatagcgcg
     1741 ggagcctggt ttgaggataa cgcttcctga tacgacagcc cggtccatga gtagccgacg
     1801 cctgccggaa gttttgcgac caggtctgcc ataaatttca tggcgtcacc ggtacttttc
     1861 ccggcagccg cttcacctaa aatctccatt gacgggatgc cgttgtagcg ttccagtcgc
     1921 ggtgaaccat aggtccattc ggtagacgaa taggcagaaa gcggtgccat cgtgccagag
     1981 gcgttgcgta cataccattg gttgatgtta tccggcaaca tacggaacgg cgtgcctgcc
     2041 tggacataca cttttttcac ccgcccctgg ttgaggaagt cgttcacgta gctgctgccg
     2101 aaggcggtgg aaattgtctg gttgatatca gacagcgcca cacccatagc ctcggctttc
     2161 gcagcgttga cgttcacttt gaacatcggc gtatcttcca ggccgttcgg gcgtaccccg
     2221 atgacctgat tcggtgattg cgctgccagt gataacagct cgtttcgcgc ctgggtcagc
     2281 ttttcgtgcc ccagattgcc gttgtccagc agttccatat caaaacctga cgcggtaccc
     2341 agttcagcca ccgcgggtaa gttgaacggg aagacgacgg ctttattgat actgcttagc
     2401 gcaatcattg cccgctgaat aatcgcggta accgagtttt cctcgccgac acgttcagac
     2461 cacggcttga ggctgataaa cgccagaccg ttgttttgcc cctgaccact gaagccaaag
     2521 ccaccaacgg taaacaccga ctgaacatta tttttctctt ttgtcagata ataatccgtc
     2581 acctgttgta gcactttcgt ggtgttaacc atggttgcac cggaaggtaa ctgcgcggtg
     2641 gtcataaata ccccctgatc ctcttctggt aagaaagagg tcggcgtacg caggaacagc
     2701 accgccatcc cggcgcaaat cagcaggtag accaccatgt agcgaccggt acaacgcaac
     2761 agcgagcggg tgctgtcggt atagtgctga gttgattttt caaacagcgt gttgaagcgt
     2821 gcgaacaggg cgttaggttt gtgatcgccc tccggcgcgg ctttcagaat ggtggcgcac
     2881 agggcagggg tcaggctcat tgctacaaat actgaaagca gcatggagga gatcagcgtg
     2941 atggagaact ggcggtagat ctccccggtc gcaccgctca taaaggccat cggcataaac
     3001 actgcggaaa gaacaacggc aatcccgacc agcgcacgtt ggatctgccc catcgattta
     3061 tgcgtcgctt ccttcggcgg tagcttatct tccgcaatga cacgctcgac gttctccacc
     3121 accacgatgg cgtcatccac cagtaaccct atcgccagca ccatcccgaa catagttaac
     3181 gtgttgatgg tgaaaccgac cgccgacaag atcgcaaacg tcccgagaat aaccaccggt
     3241 acggcaatcg tcgggatgat tgtggcgcgg aaattctgca aaaacagata catgaccagg
     3301 aagacgagga tgatagcctc aaccagcgtt ttgaaaactt cctgaataga aattttgata
     3361 aacggtgtgg tgtcgtaagg ataaaccgtc ttcaggcttg cagggaaata agccgataaa
     3421 cggttcagct cctctttcac cgctcgcgag gtatccaggg cgtttgctcc ggtagccagt
     3481 ttgatggcga tcccggcagc aggtttgccg ttatagcgcg ccacggtgga ataatcttcc
     3541 gccccaagtt cgacgcgggc gacatcacgc agcagtactt gcgaaccatc ttgctgaact
     3601 ttcaacagga ttttgccaaa ttcttccggc gtttgcagac gcgtctgcac aatgatcgag
     3661 gcgttcagtt gctggtctgc cgcctgtggc atgccaccca gttgaccacc ggaaatctgg
     3721 ttattttgca ccttaatctg ggaaataaca tcggaaggta ccaggttata tttattgagt
     3781 ttttgcgggt ccagccagat acgcatggcg tactcggaac caaagagctg tacgctacca
     3841 acgcccgcgg tacggcttag cgggtctttg atattagacg ctacatagtc cgcgatatcg
     3901 tactggttga ggctgccgtt atcagaaata aatgccgcta ccatcaggat attactgctc
     3961 gacttatcga cgctaatccc ctgctgctgc accgcttcag gtaatgaagg catagcgagc
     4021 tgcagtttat tttgcacctg aacctgtgcg atatcaggag atgtcccagt ctcgaaggtc
     4081 agagtgatag aggcattacc cgccgcatca ctggttgaag acatgtacat caggccatca
     4141 agcccattca tattttgctc aatcacctga gtgaccgagt cttctaccgt ttgcgcatcg
     4201 gcacccggat aggttgcgct gacggtaatg gtcggtggcg caatctgcgg atactgcgca
     4261 accggtaagt tcatgatcgc cagaccacct gcaagcatca taataatggc aagtacccag
     4321 gcaaaaaccg ggcgatcaat aaaatagtta gccatgaaag tccccttaag cctgcaacgt
     4381 tattgtttcg attcggtgct ggcgttttcc tggctggagg aaattgctcg tgctttgata
     4441 cccggacgaa tgcgttgcaa accggaaacg atcacccgat cgccagcctg caagccagag
     4501 gtgacaaccc actggtcgcc gatggctttg ctggcttcaa tttcgcgtag ctgcacaaca
     4561 tcgtctttat ccagaatgag cgccgttgct ttaccctggg cgttgtgggt gacgccttcc
     4621 tgcggcacca gtaatacatt ctggcggcta ccttcatcca ctaatgccgt gacgtacatg
     4681 ccaggcagca agtcaccatt tgggttgggg aaaatcgccc gtaacgtcac ggagcccgtg
     4741 gtttcatcca ccgtcgggtc ggagaatttc agcgtgccgg tctggctgta acgtttacca
     4801 ttttccagat tgagctgtac tggcgtactg ccctgaacct gtttgatttg cccactggcg
     4861 acctcttctt tcatgcgcaa gaaatcctgc accgactgcg tgagatcgac ataaatcggg
     4921 tccagacgtt gtacggtaac cagcgaatct gcctgattag cggtaacgag tgcgccgacg
     4981 gtcaccgacg atttcccgct gacgcccgta atcggcgagg tgacatttgc gtattgcagg
     5041 ttgatagtcg cctgttcaac agccgctttg gcgacggtga cattggcttc tgcttcattc
     5101 aactgggtgc gcgcggtgtc gtaatcctga cggctaacgt agttggtctt cagcaacgat
     5161 gcctggcggt taaaggtgat gcgggcattg ctggcggtag agagcgcttt cgccagcgag
     5221 cctttggcgg agttcagctc tgcctgtaaa ggcgcaggat caatctgata cagcgaatcg
     5281 ccctggttca ctttatcgcc ttcgataaag ttgcgtttaa tgataatacc gcccacctgg
     5341 ggacgtatct cggcaacttc ataaggaacg gttctaccag gcaattcgct caacacattg
     5401 accgaaccgg gggagagtgt gacgacaccg acctcaggcg tcatggcggc ggcgttttcc
     5461 gccgatttgt catcgcaggc ggtgagcatc gcgccgcaga ataacaacgg tattaacagc
     5521 tttcttcttc tgttcatttt agtccctgaa aattcttgag aaataacggt tctttaaatt
     5581 gcgcgcgagg agagatatga gggatttaca gaccagcggc atagtggggt tgtcagccag
     5641 taaggtggga tacaggcaca gtgatcgaca tggtgaggtc aacgacaaaa aaaatgttgc
     5701 cgttctgcca acagttcctg ccagcattcg ggcaacgaag ggttctactg gtggatacat
     5761 ataccagggg aataaagtcg ttttcgcctc tcctttagta atcaccgggg gtaatgaaat
     5821 cagcatctgc atccctcgtc atgccagcca tcaatttcag ttgcttatgt cctgactaaa
     5881 aataagatgt gatacccagg gtgacgatgt cgctcatacg tcttagtttc agtttgtaca
     5941 taatgttgaa tttctgaatc tttaatgttt gttgcgaaat attgagcgtc gacgtgatat
     6001 tgctttcatt tttatttttc agaatcagtt gcataatttt gaactgatta tgtgaaaaac
     6061 ataattgtga attcttatgg ggcaagtgtt taccataagt aatctcaaga agatttttga
     6121 tactttcttt gcggctgaca aaataaatag gcgctttagc ttttagttta ctgatgtgat
     6181 aaggtgccaa aacaataatt ctctcggcat ctaatttctc cagaaattta atcgcttctt
     6241 catcaaggat atgattgtga taggtatcaa tgataacctt gaaggggtta ttgcctactt
     6301 ttttgatctc tgacagggaa ttgattttta tcatttcgtg attatctttc aactgccaaa
     6361 agccctgtaa aagaaaagaa tctttcgtca tgagaaaaat cataacttgc tccttagccg
     6421 ttatcgtttc gaacatgtca tccttttcat tatttacatc cttgtccgaa tcgttgttca
     6481 atatagtaaa cgcctataag aactttgccc ttcgtgaact aatacgtcta ttaacactaa
     6541 tcaatacttc agatttatcc atcacgcact ccatatattg gtcctaagta agattcctgg
     6601 ttgttatcag tttgtaacta gtaaacaaaa ggttaacttt tgcggattac tgcccaagaa
     6661 taagttatca ataaccagtt tgtgatctct gaagaatatt actaaagtta aaatgt
//
LOCUS       DAZKJR010000209         6617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKJR010000209 DAZKJR010000000
VERSION     DAZKJR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.210"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..302)
                     /locus_tag="ACHOWS_004805"
     CDS             complement(<1..302)
                     /locus_tag="ACHOWS_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0050273.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIS"
     gene            515..1306
                     /locus_tag="ACHOWS_004806"
     CDS             515..1306
                     /locus_tag="ACHOWS_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFQ0050274.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1316..2158
                     /locus_tag="ACHOWS_004807"
     CDS             1316..2158
                     /locus_tag="ACHOWS_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0050275.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            2168..2944
                     /locus_tag="ACHOWS_004808"
     CDS             2168..2944
                     /locus_tag="ACHOWS_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFQ0050276.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            2954..4495
                     /locus_tag="ACHOWS_004809"
     CDS             2954..4495
                     /locus_tag="ACHOWS_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFQ0050277.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWMSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            4492..6564
                     /locus_tag="ACHOWS_004810"
     CDS             4492..6564
                     /locus_tag="ACHOWS_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFQ0050278.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     VHNPWGLAIVIAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPHIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMEFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDYAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
BASE COUNT         1607 a         1642 c         1758 g         1610 t
ORIGIN      
        1 ttagaaatat aataatccca tgtaaaatcg attattttct caatggcttt ctgtggcgga
       61 agatctgaga aatcaagggc cttttctttt tgtcgaatat cgttccaggt atattccagt
      121 acttcgataa ataaattttc tttattacca aagtggtgat aaaccgtctg tttactgcac
      181 cccgcggcct tgacgatatt atcgactctg gccccttcgt aaccatattc ggcaaattca
      241 ttaaccgcgc ataacagcag cttttctttc aggctctgta cgttgctggt atcaagatac
      301 ataacgcttc cccacgacct cttcgtggtg actatcataa cctgatttga tttcagcttc
      361 agaaaaaagg gcaaaaactt gtactttcat caggttatca aatgtcctgt gatcgccatc
      421 gcatatcaac caactagatg gttgatcttt ttaccctcca gtcgcaagct aatcatcact
      481 aaggaaacaa aattcaaaaa aagagagcta ttgtatgaaa tccactcgtc ctgttgccgt
      541 gattaccgga gccgctcgtg gtattggaaa gggctgtgcg ctggagcttg cgcgtggcgg
      601 atttaatctg ctgattaatg atcgtccgga tgcggatagc gtagaaaaat tacatgccac
      661 acaacaagaa tgtctggcgg aaggcgtgga agtgatttgc ttccctgctg atgtgggcga
      721 tctctccctg catgaagaga tgctcgacgc agcgcaaaac cagtggggac gtctggactg
      781 cctgctcaac aacgcaggca tttcagtgaa aaaacgcggt gatcttctcg acctcgaacc
      841 agacagcttc gaccaaaata ttgccattaa cacccgcgca ccgttcttcc tcgctcaggc
      901 attcagtaag cgcttactgg cacagccgaa accggaagca gaactgccgc accgctcgat
      961 tattttcgtc agttccatca acgccatcat gctggcgatg aatcgcggcg aatataccat
     1021 tgccaaaact gccgtctctg ccgctgctcg tctatttgct gctcgccttt gtaatgaaca
     1081 aattggcgta tatgaagtgc gtcctggtct catcaaaacc gatatgacta ttccggcaac
     1141 ggcctattac gacgaactga ttgccaaagg tctggtgccg tggggacgtt ggggttatcc
     1201 ggctgatatt gcttccactg tccgcgcgat ggcggaaggt aaactgattt acacctgtgg
     1261 tcaggcggtc gccatcgacg gcggcttaag tatgccgcgc ttctgaggct gaatcatgag
     1321 agatctgtta caacgcccgg atttattcag tatcaatacc gcgacactgg gctataaaac
     1381 gccgctaccc gccattattg atgcctgcgc cgcacgcggt attggcgcca ttgccccctg
     1441 gcgcagggag ttgcagggtg aagatctcca gcaaatcgcc cgccagcttg ctgccagtaa
     1501 cataaacgtt tccggtttgt gccgtagcac ctactacacc gcaccgacgc tggcggagcg
     1561 caagctggcg attgatgata accgccgggc gctggacgat gccgctgtgc taaatgccgc
     1621 ctgttacatg caggtggttg gcggtctgcc aatgggaaca aaagatctgt acgaagcacg
     1681 ggaacaggtg aaacaaggga ttcgccagtt gctgccacat tcgaaagagg tcggcgtgcc
     1741 aattgcactg gagccactgc acccgatgac tgctgccgat cgttcttgtc tgtgcacgtt
     1801 acgtcaggcg ctggactggt gtgatgaact cgatcccgat ggcgaatttg gcctcggcgt
     1861 ggcggtagat gtctaccatg tctggtggga cccggatctc gccagtcaga tcctgcgtgc
     1921 cggaaaacgc atcctcgcgt ttcatgtttc cgactggtta gtgccgacca ccgatctggt
     1981 caatgaccgt ggaatgccgg gagatggtgt catcaatatt ccgtcaattc gtcggctggt
     2041 tgaaaacgcc ggatttaatg gcgctattga actggaaata ttctcaccgt actggtggca
     2101 gaaagatatt aacagcacgc tggatattag cgtcgatcgc atcgcacatt attgctaagg
     2161 aataatcatg agtgaccaac ccgttctgtt cagcagagcg gcggcttctt gccgcctgac
     2221 attgaaccgt gaagataaat gtcatgctat taatgaagaa atgatcgaat ctctcgacca
     2281 ttatttaaat gaaattgaaa acgacaccac attacgatta gtcgaattaa cggcaaccgg
     2341 cgataaattc ttttgtgcgg gcggcgatat taaatcgtgg tcggcctatt cgccattaga
     2401 tatgggcaga aaatggatta aacgcggtaa cgaggttttt aatcgcctgc gcaacttacc
     2461 ccaattaacc gttgcgaatc tcaacggtca taccatcggt ggcggtattg aacttgcctt
     2521 gtgctgcgat attcgtatcg ctcgccccgg cgcgaaattc tctaacccgg aagtgatgct
     2581 cggcatggtg cctggctgga tgggcattga acgcgtgctt aatcaggttg gcccggtggt
     2641 cggtcgccag atgctgatgc tgggtaaacg cctgacggcg caggaagccc aggtggcgaa
     2701 tttaattgat gaagtggtag aaaaagaaca ggtggaaagc tggatggcga accagttagc
     2761 gcagctggaa aaatgtggtc cggtggcact ggcacatatt aagcaactga ttctggcgct
     2821 ggaaaataaa cacgccgatt actcccacca gttactggct ggattaatgt ccgccactca
     2881 ggattgccag caggcgacgc acgcatttgc cgaaaaagcc ggcgtgagct tccacaatca
     2941 ataaggaaat tgtatgcgta agttatcaac cgccgagaca ctggcggcgc aaattcagga
     3001 cggcgcaacc attgccatta gcggtaacgg cggcggtatg gtggaagctg accatattat
     3061 ggccgccatt gaagcgcgtt tcctgcaaac cggacacccg cgcgatctga cattaatcca
     3121 ctcgctgggt attggcgatc gcgacagcaa aggcactaac cgttttgctc acgccgaaat
     3181 gctcaaacgc attattgccg gacattttac ctggtcgccc aagatgcagg aactggtgaa
     3241 aagcaatgct attgaagcct actgtttccc tggtggcgta attcaggcac tgctgcggga
     3301 gatcggtgcc ggacgtccgg ggctttttac ccacgttggg ctgggatcgt ttgttgatcc
     3361 acgcaatggt ggcggtaagt cgaatgaatg cacgaccgac gacctggtag aactgattga
     3421 aatcgacggt gaaaccaaac tccgctatcg ccctttcaaa gtggactacg cgattttgcg
     3481 cggcacctat gccgatccgc gtggcaacgt tagcctcgaa gaagaagcga ttgatatgga
     3541 tagctattcg atggcgctgg cagcacacaa cagtggcggc aaagtgtttg tacaggtgcg
     3601 cgatgtactg gaagctggcg ccattgaacc tcgcaaggtc aagctaccgg ggattctggt
     3661 cgatggtatc gttgaacacc gcgaacaacc gcaaacctat cttggcggtt acgacctgac
     3721 gatcagcggt caacatcgcc gtctaagttc taacgacgct attgaactgg ttagtcatcc
     3781 ggtgcgtcgc ctgattgccc gtcgggcagc acgggaactg gtggcaggcg cttcaaccaa
     3841 ctttggcttt ggtattccgg gcggtattcc tggggttgcg ctgcgcgaag gcgtgcctta
     3901 tcaaagttta tggatgagcg tcgagcaagg cgtacataac ggcatgatgc tggatgatgc
     3961 tctgttcggc tgcgcccgta acgccgacgc cattattcca tcactcgatc aattcgaatt
     4021 ctacagtggc ggcggtatcg atatcacctt cctcggcatg ggagagatgg atcggtacgg
     4081 taacgtcaac gtatcccatc tcaatggcaa tctcattggc cccggcggct tcctcgaaat
     4141 tgcgcaaaac gcccgtaaag tggtgttctg cggtacgttt gacgccaaag gcagcaagat
     4201 tgatgtaacg cctgatggcc tgcatatcgc ccagtcaggt caaatcccta aactggttac
     4261 ccaagtggaa aaaatcactt ttagcgccgc ctacgcacag caaagtggtc aggaagtgct
     4321 gtatatcact gaacgcgcag tattccagtt aacggcagaa ggcgttgaat taattgaaat
     4381 cgcgccgggt gtggagattg agcgcgacat tctgccgtat atggctttcc gtccaattat
     4441 caagcaccca cgcctgatgg aaagtagcct gtttacgccg atggaggatg catgagtcag
     4501 cttgatgaca ccatccttga tgcgctgacg cacgttactt tcccaaaggg ttttgcacag
     4561 gcagaacccg catgggttgt cacggtagac ggtgttgatt acccactttg gcaaacagat
     4621 gccctggtag tcggcagtgg tgcagccggg ctgcgtgcag ctgtggaact taaacgccgc
     4681 cagcaaaatg tgttaatcgc caccgccggg ttatacatgg gaacatcggc ctgctccggt
     4741 tccgataaac agacgctgtt taccgccgct accgcgggca acggcgacaa cttcaccaaa
     4801 ctggcagagg cactggcgag tggtggggcg atggatcacg acactgctta tgtcgaagcg
     4861 gtaggttctc tgcacactct tggcgggttg caatatcttg gtctggaatt accggaagat
     4921 cgctatggtg cgattcttcg ttatcaaacc gaccatgacg aagccgggcg tgcaacctcg
     4981 tgtgggccac gtacctcaag gttaatggtg aaagtgctgc tggaagaagt acagcgtctt
     5041 gctatcccaa tgttgaccag tgcaacagtg attaaactgc tgcatcagcg tgacgaaaac
     5101 ggcgaagacc gcgtggcggg ggctatcctc gcgaccggtc atcgcgtcca taacccatgg
     5161 gggctggcaa ttgtgattgc gcccaatgtg gtactggcaa caggagggcc tggcgagctt
     5221 tatcgcgaca gtgtgtaccc acacaaatgt ttcggctcgc tggggctggc gctggaagaa
     5281 ggtctgacgc taaccaattt gactgaaagc cagtttggta taggcacacc gcgcagcacg
     5341 tttccgtgga atttgtccgg cacctatgtg caggtgatcc cacatatcta ttccgtggat
     5401 gccgggggca acgagtataa cttcctcgcg gattactatc gcaccaccca ggagctggct
     5461 tcaaacattt ttcgtaaagg ctatcagtgg ccgttccacg ccactcgggt aatggagttt
     5521 ggctccagct tgttagatat ggctgtagcg caagagcagc aatcagggcg tcaggtattt
     5581 atggatttca atcgcaatcc tgaagcggtg ccgggtgacc tgcccttctc attagatcga
     5641 ctggacgacg acgttcgcgc gtatctggaa aataacgacg ctctggcacc atcgcccatc
     5701 gaacggctgc aacgaatgaa tccgctgtct atctcgctgt ataagatgca cggttacgat
     5761 ctcaccacac agcctttgca gtttgccatg aataatcagc atatgaatgg cggcattgaa
     5821 gtggatatct ggggacaaac ctccctgccc ggctgtttcg ccgtggggga agttgctggc
     5881 acacacggcg tcactcgccc tggtggtgcg gcattgaatg ccgggcaggt ttttgctgtt
     5941 cgtctggcac gttttattgg ttgcacgcaa aaacgtaata ttgatggaga tatagcacag
     6001 ctggtagctc cggcactggc ttctataaga gagatcatta ctcaggcgca cgataacggg
     6061 accgggatgc cgttgtcggt tgtgagagaa aaaattcagg cacgaatgtc tgactatgcg
     6121 ggatttattt gccatgccga taaagtccga cgtgccactc gtgatgccct gctattgaac
     6181 gaatttgtcc aacggcatgg attggcaatc aaacatgtgg gtgaagttgc cgagctgttt
     6241 atgtggcggc atatggcgct gacttctgcc gcggtattaa cacaattaac gcattatatt
     6301 gatgctggtg gtggtagccg tggggcgcgt atggttattg atccacaagg caaatgccta
     6361 ccacaaactc gtcgcggcgc aaaagaagaa tggcgttttc gctctgaact cgctgaagac
     6421 aaaaatcaca aattaacaat tcaatattcg caaggttctt ttattaccga agtaaagtct
     6481 ttacgtatgc aaccgtgtat taacggtatt tactttgaaa aaaactggcc agatttttta
     6541 aagggagaca tttacacaca ataattttca atatttcctc tgtacctgat ttttattgcc
     6601 tattacatgg aaatacc
//
LOCUS       DAZKJR010000210         6605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKJR010000210 DAZKJR010000000
VERSION     DAZKJR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.211"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3649)
                     /locus_tag="ACHOWS_004811"
     CDS             complement(<1..3649)
                     /locus_tag="ACHOWS_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFQ0050279.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PLTEAIPSPPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGIMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLYFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTNDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGADGYYNATLDYSDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAVHGQLSRYTAADGAVWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAGTGAEGFRYDRHGNLLAWTDGNGVDWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYLLDNCGRVTEEHDWGGVTWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQ
                     SAGRHRISEETE"
     gene            complement(3669..4130)
                     /locus_tag="ACHOWS_004812"
     CDS             complement(3669..4130)
                     /locus_tag="ACHOWS_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFQ0050280.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRIDQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(4158..6059)
                     /locus_tag="ACHOWS_004813"
     CDS             complement(4158..6059)
                     /locus_tag="ACHOWS_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFQ0050281.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVRPPLWRA
                     GLRQNFRIFQNEDIRSILGTILQENDVTEWSPLFSEPHPSREFCVQYGETDYDFLCRI
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDWPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTEHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPKPKPSVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPANQDSSCWIRVAQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HEDNRSPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVMIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKQIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1265 a         1956 c         1789 g         1595 t
ORIGIN      
        1 gctcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccgcagc
       61 tgtcatagcc ataagtctca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctggccgttc cggtccagcc ggtactcccg gcgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacacct gacacgttac ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgacggaac cagcccgccc catgtggctt cttccggaaa aaatccggcg cgctggctga
      301 caggctggcc ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcatcgg
      481 ggtgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtaatggta ctcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgat ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca ggccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggtcacgc cgccccagtc atgctcttcc gtcacccgcc
      841 cacagttgtc gagaagataa cggtatgact caccctgcgg gttgatgact tctgtcagtt
      901 gcagcgtgtc tttatcatag cggtactgcc agcggtggcc ttcaccgtcc gttcgcgcca
      961 ccggcaaatc aaacgggccg tattccattg tccagtccac accgttaccg tccgtccagg
     1021 ccagcaggtt gccgtggcgg tcatagcgga atccttctgc gccggttcct gcccgctcaa
     1081 ggccgctcag tttatggtgc tgctgccact gcaaccgcac tgactgaccg ccggggccgc
     1141 gcatcttcac cggcaggtcg tgaccactgt attcggtgtg gtgttcacgg cccatcacgt
     1201 cggtgagtgt ttccggccgg ttgtggtggt cgtactctat gcccagacgc cgttcatcct
     1261 ggcggaatat ccccgacagc agcccctgag cggtgtacgc cagccgggtc tcttcgccct
     1321 gtggcgatac cagactcacc ggcaggccgc gttcatcgca ctgctgtgtc cacgtctgcc
     1381 cggcagggtc ggtgatgttg ctgagcaggc cgcgctcatc atagcggtac aaccacaccg
     1441 cgccgtctgc cgccgtataa cggcttaact gaccgtgtac ggcgttgtat tcaaatttct
     1501 ccacccgtcc ggcaggggag tggcgggcca gcaggtgatg gaattcatcc cattcatacg
     1561 tggtggtgct gccgtccggc gcttcttccc gcagaatatt gccgtcagga tcatagtaat
     1621 aacggtgaat gcccttgccg tccgtcacgg tggtgcagcc gtcactatag tccagcgtgg
     1681 cgttgtaata gccgtccgcc ccggtgacat tcacgcaccg gccctgttca tcgtaggtga
     1741 aacggctcca cgtctggtcg ttatcggacc agcggatgat ccggtccgca gcgtcgtact
     1801 cataaaacag gtggtagtcc agccgcgcat ccgcttcggt cagccgcccc cgcgcatcct
     1861 gttcgtaggt cgccagcgtc tgtatgccgt tatccgtgcg gtgaatggct ttcaggtagc
     1921 cgctggcgtg gtacgtcagt ttaagccgga tcccgtcggt gtgactgact cccgtgatgc
     1981 ggtactggct gtcgcgctca aacgtgatgc ggttgctggc ggtgtcgctc atcccgccca
     2041 gcatgtacat ccggtcaccg gggctgtgtg ccaggcggtc atcattggta tcgtcagtca
     2101 ccgggtggcg caggcgcatt cccggaaagg cgtcataaaa gaagcggctg ctcagggcat
     2161 cacggtcgaa cagctccagg taatcgtcgc ggcggcgcag gatatactgg gcgtgatacg
     2221 ggttaaccgc cgtatcactg tctccgtcga aggcaaagta cagcgtggct ccctggctga
     2281 tgatatccac ccggtttccc tgttcacgca cccaggcgta ttcgctccag gagtcgctcc
     2341 agcccacgcc cgtcaggcca tgcagtggca gtacagaact gtggaaacgg gtgaaggaga
     2401 gggggagtgt ctggcccagc gtaaagtcgg tacgttcgtc gcagtatgcg ccggttaccg
     2461 gatcgacagg atctgttacc catctgatga ctttattggc aatacttttt aacgacttac
     2521 ctgttttccc cagcaacgcg ccgagctttg cccccagcag cttcccgctt ttccccagca
     2581 ggtttcccag tttgccgagt ttcgccagat tccccagttt tcccagcagt cccgcgggcg
     2641 gcagaagata gctggctatc gttattcccc ccaggatcat tctctgccac ggcgggaact
     2701 ccggctggat atccagatag gtcacctgtt caccgccaat aaagacgtcc ggcgaacctt
     2761 ccacgattgc cgcgctgcac tccagtttgt cgcccttgcg cgcggcaggc tgattgttga
     2821 taaagacgtt gctgctgccc tgggctatcc tcggtggttt gttctccttg tcgcagagcg
     2881 ccgtgtcctc accggcttcc gggaaatcca cgtctttttt gatttcgcca cgcccgccca
     2941 ccatggtggc aaactccatt aataccggac cggcgggaaa cagaagcgat gcgccggcgc
     3001 tgacgcgatc cataataccg gcattgctgt ccgtcagggt gataacagcg ttcccccagg
     3061 ccttcgccag tggcactgct ttttccgctg ccgattcaag caggtcagtg gcaatatccc
     3121 cccatgaagg ttccccctgt ggtggagagg gtattgcttc agtcagggga ggagggggaa
     3181 gggtgacggc ggccctggct gctggcttac cgttaatttt tacgttttca gaaccggtat
     3241 tgatggcgcc gtccgtgtca caaaaactgt ttatccatga ctcaagctgg ttctgtttct
     3301 cctccacata atcactcgcc ttatagctgg catacatcag cgcaggcacc agaaccagcg
     3361 cataagggcc ggtggcgcag gcggccataa tcaggcaacc ttctgcgata gttactatcg
     3421 cccccagcac ggcccctgcc agtgccccca gaaaactttt atgctgaatt tcatcgccca
     3481 gcctggcggc aggtgggccg gacttctttg ctgccagcat ctgtgcaatg gctttgtcgt
     3541 tggctgcccg tttcatctgg ctggcgacca tcgcctgctg tgacagcatt cgcattgcca
     3601 gcgtaccgcc tgcggtcgct ccctgtgggc cgcctggtcc ttcactcatg tgttatccct
     3661 cgtttatgtc agcgcggctg gtccttttgg atgtcattct ccggtggcaa cggggtgaag
     3721 ctctttatca cctccagcag catcgtcttt tgactgttct tcagggataa ggaggtgctg
     3781 aaagtgaagg tcagcagcgt atctccacgc cgctggaata cggctatctg gtcaatccgt
     3841 ccctggttcc cctcatcttc cggaccgttc caggaataat ccagcagcca ggccggggcc
     3901 tctgccagag tgatttcccg gcaggcatgt tcggtgtacc catcaagttt gccctggtac
     3961 agcgccagcg tttcctggta cagctctggt gtcagggttc cctgtggaac ggcttcgcgg
     4021 gtaatgacca ggttgatgcc actgttatct gtggacacca ggacctgcat tgactgatcc
     4081 tgccattgcg atggcagctg aatatgacct tcattaaaag tgaactgcat aaaatactcc
     4141 ctgataatct gaatgaatca gttgatgaaa acagaaccgc ccacgatggt gatgccatcc
     4201 ttcgacagcc tgattaccgt cgattccccg acctgtagct gaatgccatc cggcgtcagc
     4261 gcaattttgc tttcctccac ggccagcata atttgcttaa ttgccgtata agcctgtata
     4321 ccgtcgcctt cgccaccatt gtttacagcg tcaacatgca ctgaaccggc aatcgtgcct
     4381 ctgtcctgaa aaacatcctc agataaagac ccttttagta cgcctgtgat tttgtggcct
     4441 ttttccaccg taagaaggcg gttaccttcc tggatcatga ccgtctggtc attttttacg
     4501 gtttcgcggt gatcgtgttt tacggtggtg gtacggtcgt tgagtacctc ggtatccatg
     4561 ttcttctggg cgtggatgta gacctgttct ttccctgtcg catcgtcaaa cttcagctcg
     4621 ttaaacccgc tgcccatata tgttttggag cggatggtca tctgcgtctt cgttcccggc
     4681 aggctgcccg gcgagcggtt atcttcgtgg taggtgcgcc ccataatgat cggctggtcc
     4741 ggatcgccgt tgaggaagtc cacaatcacc tcctgaccca cgcgcggtat cgccaggttg
     4801 ccaaatcccg gccccgccca cgcctgcgcc acccgtatcc agcacgaact gtcctgattg
     4861 gccgggttat agcggtccca gttgaatttc acccgcaccc ggccatgctc gtcgcagaag
     4921 atttcctctc ccgcaggccc cgtcacgacg gcgctctgcg gaccgtccac tgacggcttt
     4981 ggcttcggct gaggtcgcca cgtccggtct gccggtatta ccgcaaagtg gttattcagc
     5041 gtggtacctg accccctgcg tcccggcacc gcctgcggct gttcgccgtg cagttcgctc
     5101 gccaccacct gccattcccg gttcaggttc gcctgcggat gctccgtcag cacaattcgc
     5161 cgtccaggcc atatctccgg cgagcggctc attccccgcg cggtttcggc gttgtttcgc
     5221 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgcccgggc
     5281 cagtcataca cttcatactg tgtgcgctgg taatcctgat gctggccttc ctggtcaaaa
     5341 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacaga ggaaggacgg
     5401 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
     5461 gggttccagg ggatttcaaa ggattccggc agatagcgga ccgtgtcgca cagcaccaga
     5521 ctctggtcgg tacttttctg cgcgtgctcc tcatagaaga agatgccttc ctccgctgct
     5581 atccggcaga gaaagtcgta atcggtttca ccgtactgga cacaaaactc acgggaagga
     5641 tgcggctcac tgaacagcgg gctccactcc gtgacgtcat tttcctgcag tatggtgccg
     5701 aggatgcttc tgatgtcctc gttctggaat atacggaagt tctgacgcag tcctgcccgc
     5761 cacagcggcg ggcgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
     5821 tcaaaccagg tcaccacgcc tttcacccgg cgctgtacgt catcgccctg ccataccgtc
     5881 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctgactgacc
     5941 agagaaaggt caagggaaaa aagtgaagag agtgactggg tgagatgaaa ggagaccacc
     6001 gcaaaagcgt ccggtggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
     6061 gcaacctccg tgaaaatgaa taataatccg catatattgc atgtacaaca tgtcgggtaa
     6121 tgagataagc gggccggctt caggcgttac cggggaatat cagaaaagtg tcatggttat
     6181 gtgatgattt tgtccgggat tttacttcag agaaacgggg ggctgttgac tcaacaaaat
     6241 aatcacaaac tcaactgacg ggttcacgat gttattaagg acaggctcgt atattacatc
     6301 gagtaacaaa aaaacacctg atatgcaaag tcaaaccaat atttgtaatg tctttttatt
     6361 cacaataata aataactcaa taaacattat tgaaatgaat gatggtttgt ataaggaata
     6421 tacgctaccg ccccaccctg ataacatgat ctgcacattg ctatcttaac gtcgttagac
     6481 gctggcttaa tagtgaatat tttaaattag gaatttatca agttcacttg ataaatagac
     6541 cgcgtgaata atatagagat taatgatgcc tgactgcgct ttatattata gccgattctt
     6601 ttata
//
LOCUS       DAZKJR010000211         6553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKJR010000211 DAZKJR010000000
VERSION     DAZKJR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.212"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(116..1030)
                     /locus_tag="ACHOWS_004814"
     CDS             complement(116..1030)
                     /locus_tag="ACHOWS_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0050282.1"
                     /translation="MDKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGVAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(1090..1593)
                     /locus_tag="ACHOWS_004815"
     CDS             complement(1090..1593)
                     /locus_tag="ACHOWS_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0050283.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(1606..2136)
                     /locus_tag="ACHOWS_004816"
     CDS             complement(1606..2136)
                     /locus_tag="ACHOWS_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0050284.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(2150..4801)
                     /locus_tag="ACHOWS_004817"
     CDS             complement(2150..4801)
                     /locus_tag="ACHOWS_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFQ0050285.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGHEAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVSDNSACTPLRDRLADASSEFNVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKDADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEEYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPAVSPETLLNQQTAICR"
     gene            complement(4843..5553)
                     /locus_tag="ACHOWS_004818"
     CDS             complement(4843..5553)
                     /locus_tag="ACHOWS_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFQ0050286.1"
                     /translation="MQTTRPPYSVSFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAASGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(5916..6479)
                     /gene="fimA"
                     /locus_tag="ACHOWS_004819"
     CDS             complement(5916..6479)
                     /gene="fimA"
                     /locus_tag="ACHOWS_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFQ0050287.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         1812 a         1457 c         1404 g         1880 t
ORIGIN      
        1 ctctggcgtt aattgtgcgc ggattttctt acaggtgtag gataatagcc ttcacaaaat
       61 aattatccgc gcaattttcg cgaatggagc gcatagacta aggtcaaaat cacaattatt
      121 catagataaa agtgacacca atgactgact gaactgttcc ggctgaaact ttgtttcctg
      181 tttgtccata ggttgcgctt aaacccagag gtactttgct tttatttact gtccccaggg
      241 agacgttttc tcccgtagcc agtatttttc cattacgcat cagagagaca cccactcccg
      301 atgcttttgt tgcatctggt gctgtgttag caaacacttg tctggcactg tcagtggtag
      361 cacccgaaag ataaaatgag agcttttgtt cacttgagca atatacgcca aggggtattt
      421 ctgcgcttcc aggaaagtcc gggagattca cgttgacatt acgactatca acggtgcagc
      481 cgcctgttgg cataacaaca ctgttatttg aaataatatt ccacgtaaaa ttacgcggat
      541 ttccgcttcc taacgtggca attttataca tatgaatacg ggcaataact tcccctgctt
      601 ttatgacaac tccaccggcc actccgactg gggtaatata gagttttaac ggtaatggca
      661 tcggtgtctt atcaccaata tccaatacgt tggtatttgt cgtcaacggg aaggggtagg
      721 tcacattatt ccagtacagt gatcctttat atgactgtag ggagcctgcg aaagctgatc
      781 cttgtaccag gtttatatga tcagtgtcgt accagccgcc gtaatcattc cagcaactta
      841 tatgctgaga caagtctaca accagattct ggcccggttg tattacagga tcgagattaa
      901 cataaacaga ggttgtacct gctccaatac ttgacccgcc atcgacatta caactgaagg
      961 caaaaacttt gcctgccatt aatagaagat aaataccgaa caggaccttt atactgaatg
     1021 ttttatccat gatatatcct aaggttaaaa aattgattta aaaaggaggc aaaaaaaatc
     1081 aaattgatgt tattgccagg tatagatcac attgattagc gcctcgatcg ttccctggct
     1141 tgcgtttcca ttcaccgtga tagctcttgc cttaagtgga aactgtgcat tacgagtgat
     1201 ctcatcaaca ataaccgttt tgctatcgcc attttttaac gtcgcatcct ggtcatccct
     1261 tagctctatc tgaatatttt cggcagtacc ttcatttttg taataacccg tattgtcagt
     1321 tgaacctgtc acgattgccg tcactgcact tgtttcaacc ggacaatcgg ttaatgacaa
     1381 agtaatattg tgccagcctg atgcagaacc aggttgttgc agattgcgcg tataaagatc
     1441 cccgagatta acgttagctt ctttggtttg aatagtgcag ggtttagcga ctacccgacc
     1501 attaacagtg atagtgacat cggcggcctg gagagttgct gtgaataacg cgcaaaacgt
     1561 cgctaacagg aacctcttgt gaagtctttt catatctggc tctgattatt gaaattcaag
     1621 ggtaaaggtt gccgaagccc tcaccagtcc cggattgacg gacttctgag ttgacttcag
     1681 acgagcggag taaggcaaaa tattgttctg ttctggtacc agtgggatcc actgcatccc
     1741 ggcatgaagg tcattcaatt tcaccggacg catatttccg tccagtattt ctatacccaa
     1801 accggaggcc gtgttgcttc cttcatccag tttcaacaga gtattatttt ctgcatcctc
     1861 aataccgtta aatgcaaccc gaacccccgt tgtcccttta ctgcattcac ttaacgtaat
     1921 ctgaaaaggg acggctggac ttgtgctacc ggtcgttgga aattgtctgg cactgttttt
     1981 ttggagatct acggtaaaat taagcgaatc cgatgagact gtgcagccat aatcgagaac
     2041 gcgcccgcta attttaataa cgctatctgc agataaagca gaataggtgg ttaaccccag
     2101 acataaaccg aggaaaataa cgttattgta tttcataatc tattgttcct tagcgacaga
     2161 ttgctgtctg ctggttcagc aaggtttcag gagagactgc aggaagcttg tactcgacaa
     2221 tacagtttga gtttttatct ttgccccatg aaacctgtaa tttccctgac tgtggaagtc
     2281 cagtcagata aacctgaccg ttttccgcga caatgctgcc atttttattc tccccatgtg
     2341 tgacaattgc accgaatgga acgctcttat taccgtactt caacgtcatt aatactttcc
     2401 cgccgatttg tgcattaaat gttgctctgg caatagcacc gtgagttggg atgacagtga
     2461 caacggtttc atccagttca acattatctg caagggaatt cgcgttaaga gcgacacgat
     2521 tttctctata ttctgtcgca aatggtaata tggcatagcc acgccagtcg gtatgaattc
     2581 cggtctggtt ctctattttg acattatcag cgccaggagc cttaaccaga accattgtgt
     2641 cgcccagcgg ctgtccaaag gtgatgccat cagcatgagc aataatgcca ccactcattc
     2701 cgtaataaat ctggctgctg tcaccactcc gactgtaacc gacattcgta ttaccgtaag
     2761 ctccacgata attaagagaa ctgtaaccac tggtgccaga cgatgtatta cccccttggg
     2821 tgttaccgac ctgaacgcta taattcaggt tattatccgg cagcagagtg ccataaaccc
     2881 ctgataggtt ggtcatgccg cctttcaaat cgtttgacat actgtaactg gcgtttgaat
     2941 tacgaaatgc cgactgactg tctgtacgca tccaatgact gaagggaaca ttaagcgtaa
     3001 aagcaagtaa atgatcccga tcgttttgcc atatattatt ggaatagctg taattcagcg
     3061 aagtcgtaat atcaccaaac ggcacattta atccaaatga tatttgctgg tcgctgcgtg
     3121 acgtgttcca gtaactttgg cgactggcac tgaaaaatgt cgtaccgtaa tgtccaagct
     3181 gctgagagat gcttatttgt tcctgaccac gcttactgta aaacagatta aaataatcaa
     3241 taaatagtgt ctgctcattg ctgtctccgg taggaggctt gacggtgtaa ccactcattc
     3301 gactgtaggc actgtcggat aagttataaa aaccttgcgt agaatagcga tatcctgcga
     3361 cctggatatt ggttcccgtc tggtagaagg atttgctgta gacggattta accgattgtc
     3421 cgctgtgacg ggtgtcatct gcaagcgtcg tattggcctg cgtgatatcg aaagaaaagg
     3481 caccaaaaag tcctaaatct ttaccaatac caaggttgaa ggcctgatat tcttctgcaa
     3541 tttgcattcc gccataaggt gtccagtttc cttccagtcc atgcatcaag ctaccttgta
     3601 tgaatttggg ggagctttgc aggttatttc cactacgata ttcccccatg gcaagcgcat
     3661 aacgcgtata tccagcacgt tggagaaccg gaacagacga ataaggaaca tataatgtct
     3721 gaatactgcc gtctgcatct tttatggtta cttgcagatc accaccattg gccgcagagt
     3781 tgatatcatc aatagtaaaa gggccgggtg ggacagtagt ctgataaaca tcgtatccat
     3841 tctgttttac actcacttgt gcggtgccgc gggcaatacc atgaatcacc ggagcgaaac
     3901 catgttggct atcgggcaac atcgcttctg ttgaatttat tttgaggcca cgaaagttta
     3961 cactatcaaa aatatcgcca tcggtataac tatcacctac cgttaagcgt gagcgtaagg
     4021 gaataatgtc acgttcagcc gacgtattga tatgctgcca tttattgctg tcagatgaat
     4081 tgctactccc actgttataa ctccacgttg agttatcgcg cagtcgccaa ctaccaatgt
     4141 tgatgccact ctgtaaattc aaatatgcat agttggattt tcctgcatta tggttactac
     4201 gattattaat agaattacca ttaaaactat agtttagtag cccagcattt attccttctt
     4261 cccacagatc cggagaaaca tagccgcgag ccattctgcc aacataaatt tgcggaacgg
     4321 agagtgatag atgttgctga ccaacattaa actcagatga tgcgtcagcc aaacgatccc
     4381 gaagtggtgt gcatgcgctg ttgtccgaca catgtttttc tgccgaatgt tccttattgt
     4441 ctaataatgc agattttttt atgccaagac tgacaagaag atctgtactc aggcatggga
     4501 tgagatctgc gttattatca tctgcaataa aagtaatatc ccgactagtc ataaattcgt
     4561 cgttcaaata aatgtcgacc cgatatgtac caggtggtgc ctcgtgtccc ttagtaaagg
     4621 cggataaatc gacagattca gcaagatcat tgcttaaaaa ccgtggatta aaataatact
     4681 ctgcgtcaac gggaaaaacc gcagttgcta agccaagggc aatggtaagc cgtgcaaagc
     4741 gaggcagtag taacacctga tgcatacgat atgccttaaa tgcggcacga aaggctgtca
     4801 ttattaattc cgattgagtt tattattaat taagtcactg ctttactgca tggttgcctt
     4861 aatacgcgga gttaatgcac cgtaatcatt gatcgtctga aaactgatat ctccggatgc
     4921 ggcatgcggg atatccaccg atacttcacc ttttggcggc accatggtgt ttgggagatt
     4981 gctatttcct gctttcatat tcgttacggt aatgaagtag ggcgttggat taataagagt
     5041 aagtttgcta ccgctgcgac gaaaccgaag cattgcaggc gcttcttcag gtgccattgc
     5101 caggttcgtg gggcgataaa acatttttat gcggctaata attgccagct gtaatgtatt
     5161 ctcattcttc tggtcttttt ccatagcagg aatagcttta acattcaccc agaacaggct
     5221 ttcacggtcg cctggtaact gatgattagt ggcgttgatt atccgcagcg tgttctcttt
     5281 tttaccttgc atggaaaaaa gcggtggcgt aatgacaaac tgggtgtctt tttggtcacc
     5341 cgcattttct atccatgact gaatgagata gacactggca ggatcattat tcgtgacagg
     5401 caataaaacc tgtttctggt ttgccggata aattacccgc gtcgcaccca gtgcgacagc
     5461 tgcgttggca acggtgctat ggcaagcaaa gagcaataaa agtaacactg tcgccataaa
     5521 ggaaacagag taggggggac gcgtagtttg catctgatgc attctccatg tgttaagagg
     5581 ataagcgggg aaaccgttac atcagacaat cggtaaatgt tgctaataca ttggcgagtc
     5641 gccgggtttc cagacttaat gcgtttgaaa taaatgttgg ttctctttta ataggaaaac
     5701 cttgctataa agtaaatatt ttaaataaca gtaagtttaa ctactgttgt ttattgaacg
     5761 tttagatatt atgtgatttt ttcataccgc tccaaaacac aaactattcc tcattaataa
     5821 tgagatgaac attggtgaat ttctcgggtg tgcgtagcca tgccacacac accctgtttc
     5881 aatttcagat gacgtatagc catcttaaga ttgaattata gatattgaac tttgaaggtc
     5941 gcatcagcgt ttgcagtacc agctactgac tgcccgagag caatataacg agcctggaac
     6001 ggcaatatat tcgtgccatc aatcaagtca gttttagcgc taaaagtggc accatcaagt
     6061 attaatacct cccccgtacg gtccaaaatt tgaataccga cattttgggc agagcctgcc
     6121 gctgaactct gcagcgctaa cgtatcgtca ttactggtga cagtagtacc caggaatgca
     6181 acagctgcat tactggaaac attggtatca caatcattca gcttgatatt gaagccaacg
     6241 gcggagctga ggtcacccgc tttagccaga cgagaagaac ggacctgacc cagctcaacc
     6301 gtttggttca ttgattctga atcaatcgca caagcggcat ttaccacttc acctttaaaa
     6361 tgaataacac cgccattaac agtggtcgtt actgattcat caccctcggc tgcgcttacc
     6421 gcagcagacg acatcgccaa tacagaaacg acaatcatac cgacatgttt gagtttcata
     6481 attctccaaa atttatattt aagtagctga ataaagagag ttaacaatta agtcattctg
     6541 ttattgctta taa
//
LOCUS       DAZKJR010000212         6539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKJR010000212 DAZKJR010000000
VERSION     DAZKJR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.213"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            179..829
                     /gene="csgD"
                     /locus_tag="ACHOWS_004820"
     CDS             179..829
                     /gene="csgD"
                     /locus_tag="ACHOWS_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFQ0050288.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            834..1223
                     /gene="csgE"
                     /locus_tag="ACHOWS_004821"
     CDS             834..1223
                     /gene="csgE"
                     /locus_tag="ACHOWS_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFQ0050289.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1248..1664
                     /gene="csgF"
                     /locus_tag="ACHOWS_004822"
     CDS             1248..1664
                     /gene="csgF"
                     /locus_tag="ACHOWS_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFQ0050290.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1691..2524
                     /gene="csgG"
                     /locus_tag="ACHOWS_004823"
     CDS             1691..2524
                     /gene="csgG"
                     /locus_tag="ACHOWS_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFQ0050291.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2588..3079)
                     /gene="ycdZ"
                     /locus_tag="ACHOWS_004824"
     CDS             complement(2588..3079)
                     /gene="ycdZ"
                     /locus_tag="ACHOWS_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFQ0050292.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(3181..3735)
                     /gene="ycdY"
                     /locus_tag="ACHOWS_004825"
     CDS             complement(3181..3735)
                     /gene="ycdY"
                     /locus_tag="ACHOWS_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFQ0050293.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3759..4496)
                     /gene="ycdX"
                     /locus_tag="ACHOWS_004826"
     CDS             complement(3759..4496)
                     /gene="ycdX"
                     /locus_tag="ACHOWS_004826"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFQ0050294.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4551..5489)
                     /gene="ghrA"
                     /locus_tag="ACHOWS_004827"
     CDS             complement(4551..5489)
                     /gene="ghrA"
                     /locus_tag="ACHOWS_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFQ0050295.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            5722..5809
                     /locus_tag="ACHOWS_004828"
     tRNA            5722..5809
                     /locus_tag="ACHOWS_004828"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5756..5758,aa:Ser,seq:gga)
     gene            complement(5959..>6539)
                     /locus_tag="ACHOWS_004829"
     CDS             complement(5959..>6539)
                     /locus_tag="ACHOWS_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFQ0050296.1"
                     /translation="NLDREIWRDLMQCSGMLSLMDAQARDTWYRSLEYDNFPEISEEN
                     ILSTFEQLHQNKDEVFERGVINVFRGLSWNYKTNCPCKFGNKIIVNNLVRWDQWGLHL
                     ITGQQADRLADLERMLHLFSGKPIPDNRENITIHLDDHIRSLQGKECYEDEMFIIKYF
                     KKGSAHITFRKPELVDRLNDIIAKHYPDMLAV"
BASE COUNT         1750 a         1609 c         1489 g         1691 t
ORIGIN      
        1 atttaagatt tgtaatggct agattgaaat cagatgtaat ccattagttt tatattttac
       61 ccatttaggg ctgatttatt actacacaca gcagtgcaac atctgtcagt acttctggtg
      121 cttctatttt agaggcagct gtcaggtgtg cgatcaataa aaaaagcggg gtttcatcat
      181 gtttaatgaa gtccatagta ttcatggtca tacattattg ttgatcacta aaccttcttt
      241 gcaggcgaca gctctcttgc agcaccttaa gcaatcgctg gcaattacag gaaaattaca
      301 taatattcaa cgttctctgg acgatatctc ttcaggctct attattcttc tggatatgat
      361 ggaagcggat aaaaagctta tccattattg gcaggatact ttgagcagga aaaacaacaa
      421 tatcaaaata ttgttgctaa atacgcctga agattacccg taccgcgaca ttgaaaactg
      481 gcctcatatc aacggcgttt tttatgccat ggaggatcaa gaacgtgttg tcaatgggtt
      541 gcaaggcgtc ctgcgcggcg aatgctactt tacgcaaaag cttgccagtt acctgattac
      601 gcattcaggt aactatcgtt ataacagcac ggaatcagcc ctccttactc atcgggaaaa
      661 agagatcctg aataagctgc gtatcggcgc gtctaataac gagatcgctc gttcgttgtt
      721 catcagcgaa aatacggtta aaacgcatct ttataatctt ttcaagaaga tagccgtaaa
      781 aaaccggaca caagcggttt cctgggcaaa cgataacctc aggcgataaa gccatgaaac
      841 gttatttacg ctggattgtg gcggcagaat ttctgttcgc cgcagggaat cttcacgccg
      901 ttgaggtaga agtcccggga ttgttaactg accatactgt ttcatctatt ggccatgatt
      961 tttaccgagc ctttagtgat aaatgggaaa gtgactatac gggtaactta acgattaatg
     1021 aaaggcccag tgcacgatgg ggaagctgga tcacaataac ggtgaatcag gacgttattt
     1081 tccagacttt tttatttccg ttgaaaagag acttcgagaa aactgtcgtc tttgcactga
     1141 ttcaaactga agaagcacta aatcgtcgcc agataaacca agcgttatta agtacgggcg
     1201 atttggcgca tgatgaattt taaataataa attgttcgga ggctgcaatg cgtgtcaaac
     1261 atgcagtagt tctactcatg cttatttcgc cattaagttg ggctggaacc atgactttcc
     1321 agttccgtaa tccaaacttt ggtggtaacc caaataatgg cgcttttttg ttaaatagcg
     1381 ctcaggccca aaactcttat aaagatccga gctataacga tgactttggt attgaaacgc
     1441 cctcggcgtt agataacttt actcaggcca tccagtcaca aattttgggt gggctactgt
     1501 cgaatattaa taccggtaaa ccgggccgca tggtgaccaa cgattatatt gttgatattg
     1561 ctaaccgcga tggtcaattg cagttgaacg tgacagatcg taaaaccgga caaacctcga
     1621 ccatccaggt ttcgggttta caaaataact caaccgattt ttaagcccca gcttcataag
     1681 gaaaataatc atgcagcgct tatttctttt ggttgccgtc atgttactga gcggatgctt
     1741 aaccgccccg cctaaagaag ccgccagacc gacattaatg cctcgtgctc agagctacaa
     1801 agatttgacc catctgccag cgccgacggg taagatcttt gtttcggtat acaacattca
     1861 ggacgaaacc gggcaattta aaccctaccc ggcaagtaac ttctccactg ctgttccgca
     1921 aagcgccacg gcaatgctgg tcacggcatt gaaagattct cgctggttta taccgctgga
     1981 gcgccaaggc ttacaaaacc tgcttaacga gcgcaagatt attcgtgcgg cacaagaaaa
     2041 cggcacggtt gccattaata accgaatccc gctgcaatct ttaacggcgg caaatatcat
     2101 ggttgaaggt tcaattatcg gttatgaaag caacgttaaa tcgggcgggg ttggggcaag
     2161 atattttggg atcggtgcag acacgcaata ccagcttgat cagattgccg tgaacctgcg
     2221 cgtcgtcaat gtgagtaccg gcgagatcct ctcctcggtg aacaccagta agacgatact
     2281 ttcctatgaa gttcaggcgg gggtcttccg ctttattgac taccagcgct tgctggaagg
     2341 ggaagtaggt tacacctcga acgaacccgt tatgctgtgc ctgatgtcgg ctatcgaaac
     2401 aggcgtcatt ttcctgatta atgatggtat cgaccgcggt ctttgggatt tgcaaaataa
     2461 agcagaacgg cagaatgaca ttctagtgaa ataccgccat atgtcggttc caccggaatc
     2521 ctgatagata aaaatgccgg aattatcatc cgacatttta cggtttaagc gaaagcgtga
     2581 gtgcccctca cgctttattt ttgatttgct gctcacggtg agccgtcttt gcactacgcg
     2641 ccgccaacca caggccgctg tttttcattg cgtaaccaaa taccagtcca agcgccagcg
     2701 aaggtaacac cagtttccag tcaccctgcc cggcaaacgt cgcgcatgcg cctataaacg
     2761 tccccggaac aaatgaaagc agcagctgtt tggcctggat acacatcaga aacgcgacaa
     2821 taccggttat ggcataaccg agtatttcca gatgtggtgc cagtgcacta ccgtaaataa
     2881 tgaccatcgc ccacaccacg ccactaagca gggttgcagc ggagatcgcc agccctttca
     2941 gcccaccttg tgggcaggca aagtaggccg tacagcccag gaagcccgcc cagcttagta
     3001 agcccaggga aacagccacc catccccaga taccggagag aatgcctgtt gtgattgcaa
     3061 tagagagaag tatgttcatg ccgcgcatga tagcagaaaa cgcggccatg aatgagatcg
     3121 agcacacatt ttatgcaaca acaaattacc cgttgcagtt atgagtgatg cagatcacat
     3181 ttactcttca gaatcttctt ccagctcgtc ccacattgca ctaatagcat cgcgggttag
     3241 cggtgccatg gtgcgccaga aaggcgtagt tgcatgggcc tccactttgc caaggaacgc
     3301 accacaccag ggtaacagat actcactgaa cagtgtttcc agtgcttcgc tctcatcttc
     3361 cgttgactga tcttccagcc aggaagccgc aagcagcaat gtgccgatgt gatcggctgg
     3421 cgtatccgct agtggcatcc ctcgttcgga aagaaaagcg cgcacttccg cttccgttgc
     3481 gccctcaacc catgcgctac gatatggcgg cacagcacat tcatcgccga taaacaacgc
     3541 attgtaatcg gcagagactt gcgtcatatc acagcttttc tgtaaacgcg tcagtaactc
     3601 atcctgctcc agtggccagt tcgcagccag tttcccctca cgaatcaggg taaacaacgg
     3661 caccagtaaa ggatcttgcg gttggcggta atagagcgaa cccagcacac gacagaggat
     3721 agaaaactca ttcattaata tatttccatt actagtaatt aaaggtctgc aaattccgca
     3781 atcggtgcca taccgcgaga ttcaaggaag ttaagtaagc ggcgcggaga aacattcaaa
     3841 atgcgctctg gcggaaaatc taccgcgtcg aggattttaa gacactcttc aaattccccc
     3901 atggtaaacg cagtgtgaga atccgagcct aatgccaccc aacctccagc gtcacgtacc
     3961 gctgcggcta ccgcccgaca gttgtcttca ctgcccttac gtgagtgtaa aaatgaggaa
     4021 ttattgattt ccagcgccac ctgatgtttc gcggctgctt cagcaacggc tttaacatct
     4081 atttcatatt tcggatttcc gggatggctt attatatgca cattgccgct ggcgatagtg
     4141 gcgatcatcg cttgcgtatt ggtcgctttg tcatgcggcg caaacaccgg ctcatgaaaa
     4201 ccggcaataa tcagatccag cgaatcaaac attttgccac tgcagtcaat ttcaccgtca
     4261 acatttttaa tgttagcttc gatgccgcgc aggatcccaa ccccatcaac cactcgcggc
     4321 cagatacgca tgttaatgaa gtgccagtga tgcggcgcat cttccatatc cgggccatga
     4381 tcggtgatcg caaaaagttt aatgcccttt tgtttggcct gggcaatgta atcactcaat
     4441 gtgctatatg catgcgtgct ggcaacggta tgcatatgaa ggtcgacggg atacataact
     4501 ctctcctgta ttcaattccc gccaaggata gcaggaaacc tgatgcttta ttagtagccg
     4561 cgtgcgcggt cgacttgccc gcagaccctc tccccttttt cgagctgggc aatggtgcgc
     4621 gaaatgtaat ccacagcttc agcgggacgg gtaatcgcgg cgacatgtgg tgttatcgtc
     4681 acgcgtggat gttgccagag cggactttcc ggcggtaagg gttcacgatt aaaaacatcc
     4741 agcattgcgc ctttaacttt gccgctatcc agcgccgcga gcaggtcatc ttccacgaca
     4801 tgaacaccac gcgccaggtt gaggagatac gcgccatccg gtaatttttc gagtaattgt
     4861 tgattaataa tgccgacggt ttcaggtgta ttcggtaaca aattaatcaa tacccgacat
     4921 tggctcagaa atgcagagag ttcttcccgt ccggcaaagc tttgcacgcc aggccacgat
     4981 ttacgggttc gactccagca acgcagcggg aagcgccagg tttgcagact ctgtgcaact
     5041 ttactgccca gtacacctgc acccaaaatg ccgatggtaa aatcttcccg atgatattca
     5101 ggcagcggtt gccaatgcga actattttgc tggatgcggt aatcgtcaaa acgtcgaaac
     5161 caatgcagca cctgactgac agcatattcc tgcatttgct cgcccatacc ggtatcttcc
     5221 aggcgaaaaa gtggaacaga aggcttcagc atttcagggt gagcctgtag cttgctcaaa
     5281 atagaatcaa cacctgcccc aagtgcaaac accgctttaa gatcgcgccc agccagcatt
     5341 tcaacaggag gatgccagac taaagcataa tcggctgatt cattgtctcc gcttttccat
     5401 gctctgactc ttgcctgagg aatagctttg tgcagtgcct caatccacca ttgggtatcg
     5461 aacgttgggt gataaaagat gatatccata ctgactcccg aagagtgttg gcgaaattta
     5521 ttcgcactta tcgttatgat ctacaaagac cagcagcata acaaatccgt ggtcggtggc
     5581 aaaaaaagca aatttcgctt attaaaactg catattgatt gaagtttgaa taatcgagcg
     5641 attttttaaa aaagtttgtt gacctcaggt catgatttcc ctaaattagc gcccgttcca
     5701 gcaagacagg aacgacaatt tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa
     5761 gtgtgtatac ggcaacgtat cgggggttcg aatccccccc tcaccgccag attacaagcg
     5821 catgttattg tttaacatgc gcttttttat ttatccttcc agacagtatg ccaccgggta
     5881 tgccatctga aaccggttta tatgccccac gatcatatct tatcggacaa tttccgatct
     5941 ttgagcatac tggcaaagct atactgccag catgtcagga tagtgttttg cgataatatc
     6001 attcagtctg tcaaccagtt ctggcttcct gaacgtgatg tgcgcagagc ccttcttaaa
     6061 gtatttaata atgaacatct catcttcata gcattctttt ccctgaagag accggatgtg
     6121 atcgtccaga tgaatagtta tgttttccct gttgtcgggg atcggtttgc cgctgaacag
     6181 atgcagcatt ctttccaggt cggcaagtct atctgcctgt tgcccggtga taagatgcaa
     6241 tccccactgg tcccacctca ccagattgtt gacgataatt ttattgccaa atttacaggg
     6301 gcaattggtt ttgtaattcc agctcaaccc tctgaagacg ttgatcactc ctcgctcaaa
     6361 cacttcatcc ttattctggt gtagctgctc aaacgtactc agaatattct cttcactgat
     6421 ttccggaaaa ttatcgtact ccagtgaccg gtaccatgta tcacgggcct gtgcatccat
     6481 taaggaaagc atcccggagc attgcatcag gtcacgccag atttctcgat cgagattgc
//
LOCUS       DAZKJR010000213         6501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKJR010000213 DAZKJR010000000
VERSION     DAZKJR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.214"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..863
                     /gene="rcnA"
                     /locus_tag="ACHOWS_004830"
     CDS             39..863
                     /gene="rcnA"
                     /locus_tag="ACHOWS_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFQ0050297.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGKRNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1082..1420
                     /gene="rcnB"
                     /locus_tag="ACHOWS_004831"
     CDS             1082..1420
                     /gene="rcnB"
                     /locus_tag="ACHOWS_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFQ0050298.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPDMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1502..2536)
                     /gene="yehA"
                     /locus_tag="ACHOWS_004832"
     CDS             complement(1502..2536)
                     /gene="yehA"
                     /locus_tag="ACHOWS_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFQ0050299.1"
                     /translation="MEIRIMLFIVMMMVMPVSYATCYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKPDDLLINLESIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAEDNGVGFML
                     ESNSSPVTFLNITDNSKGYTNLKEIAAKSKITDTTVSIPVTASYYVYDKNKVKSGALQ
                     ATALINVKYD"
     gene            complement(2552..5032)
                     /gene="yehB"
                     /locus_tag="ACHOWS_004833"
     CDS             complement(2552..5032)
                     /gene="yehB"
                     /locus_tag="ACHOWS_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFQ0050300.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSIQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPFRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCAITFGKEIDESRNYICQ"
     gene            complement(5048..5722)
                     /gene="yehC"
                     /locus_tag="ACHOWS_004834"
     CDS             complement(5048..5722)
                     /gene="yehC"
                     /locus_tag="ACHOWS_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFQ0050301.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSENWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(5803..6345)
                     /gene="yehD"
                     /locus_tag="ACHOWS_004835"
     CDS             complement(5803..6345)
                     /gene="yehD"
                     /locus_tag="ACHOWS_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0050302.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDISAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         1784 a         1549 c         1267 g         1901 t
ORIGIN      
        1 agaatcagat tgccgaatta atactaagaa ttattatcat gaccgaattt acaacacttc
       61 ttcagcaagg aaacgcctgg ttcttcatcc ccagcgccat cttacttggt gcgcttcatg
      121 gcctggaacc ggggcactca aaaacgatga tggcggcgtt tatcatcgcc atcaaaggca
      181 ccattaaaca ggcggtgatg ctcggactgg cagcaactat ttcgcatacc gcagtagtct
      241 ggttaattgc ctttggcggg atggtgatca gcaaacgctt tactgctcaa tcagcagaac
      301 cgtggctcca gttgatttcc gcagtgatca ttattagcac cgcgttctgg atgttctggc
      361 gtacctggcg cggcaaacgc aactggctgg agaatatgca cgagcatgat catgagcatc
      421 atcaccacga tcacgaagat caccacgacc atggacatca tcaccatcac gaacatggcg
      481 agtatcagga tgcccatgca cgagcccatg ccaatgacat taaacgacgc tttgatggta
      541 gagaggtcac caactggcaa attttgttat ttggcttaac cggtggcctt atcccctgcc
      601 cggcagcaat taccgtgctg ttgatttgca ttcagttgaa agccctgaca ctgggcgcaa
      661 cactggtcgt cagtttcagc atcggcctgg cgttaacgct tgtcaccgta ggcgttggcg
      721 cagcaatcag cgttcagcag gtcgcaaaac gctggagcgg atttaacact ctcgctaaac
      781 gcgctcccta tttttccagt ctgttgattg gcttagttgg tgtgtatatg ggcgtacatg
      841 gcttcatggg cataatgcga taactcaatc ctttaggctt gcatgttatc ttcagcccag
      901 gatgattctt aaatcagcta tttccgctga ctctctgctc gctaaggatg cttttagggc
      961 atcctttcat ttacactttt tacgaaatca tgggatcact aacaaaatat cgcttgtcag
     1021 ttatattgta tggcaggaaa gatatgcgac tgatattaca gatccccaaa gtggagagtt
     1081 tatgaccatt aaaaataaga tgttgctggg tgcgcttttg ctggttacca gtgccgcctg
     1141 ggccgcacca gccaccgcgg gttcgaccaa tacctcggga atttctaagt atgagttaag
     1201 cagtttcatt gctgacttta agcatttcaa accaggcgat accgtcccag acatgtaccg
     1261 caccgatgag tacaacatta agcagtggca gttacgtaac ctgcccgcgc ctgatgccgg
     1321 gacgcactgg acctatatgg gtggcgcgta cgtgttgatc agcgacaccg acggtaaaat
     1381 cattaaagcc tacgacggtg agatttttta tcatcgctaa aaaaagcccc ctcatcatga
     1441 gggggaaatg cagacacctt gttatttttt attattcgcc acttgctcgt cttgcttatt
     1501 attagtcgta tttcacattg attaatgcgg ttgcctgtag tgcaccagat tttactttat
     1561 ttttatcata aacatagtaa ctggcagtta ctggaattga gaccgttgta tctgtaattt
     1621 ttgacttcgc cgcaatttct tttaaatttg tgtacccttt gctgttatca gtgatattta
     1681 aaaatgtgac tggcgaacta ttgctttcaa gcataaatcc gactccatta tcttctgcac
     1741 ctttcaggat cgtgttactg ccggctaaca aatcagaact tgataaatac acctgcattt
     1801 ttcgtgatgt ttgattttgt tgacctaata aatttttaca acgcagaata atatcccctt
     1861 ttttactatt tattgttgct ttgttacctg tggctggtaa ttgtgaaacg ggaatactct
     1921 ctaaattaat taataaatcg tccggtttgc aggttgtggt tatcgggttg tatgacaatg
     1981 ccaaattaaa ggaatagaca cctgcatttt gtgccaatgc atttctgtag ctatttacac
     2041 atgcatccca tgacttgcca cctaataaac acagtagaaa ttgtactgcg gcactcccgc
     2101 gtgtcgatga tgaagcactg gataatgttg caacaccatt aatcatcact gaaccgccgc
     2161 cccccgcaga aacattcact tttgcattac tggccgcagg tgtcaccgtt accgaataac
     2221 cactggtgaa actggtctga ttatttgaca acgtaatgtt gtttttattg gtccattcaa
     2281 aatgtaaatt taatttaatg gtatcgttat aaagtccaac aataatatcc gacgggctca
     2341 taggtgtgat agtatttgta ctcacgcatg acgaatcatt aaaattatgc tcatatgtcg
     2401 ccatttgtgt ttgagtaagt gcaaaatccc cctgaacaac caaattgtgc tgaacagata
     2461 actcactata acatgtcgca tagctcacag gcataaccat catcattact ataaatagca
     2521 ttatgcgaat ttccatagaa ccacctgact attactggca aatataattt ctactttcat
     2581 caatctcttt accgaaggtg attgcgcatg aaagcccttg ttgcttatca attgccacat
     2641 taaccgatgg cggtacttca ttggtgcgaa taaataactg gcttccctgg ccgacaacac
     2701 caatattatg accatggata tcattgactt cataaccaaa cgttaatggt tgcccatccg
     2761 ttcttaacgc ttttataaac cagggcttgc gctgatcggt atcaaaatta accagtacaa
     2821 ccgcgccgcg aaaaggggcg gcaattttcc ggttgccacg taattctgct tcgctatcgc
     2881 tttgcgacac atccagcatc aggtgatttt cccgataagg tgtcattccg tcgtatacca
     2941 ccactccatt acggtttgtt gtgcgatatt tttgcccatt gacataagca tctttaattc
     3001 ctggcgcatt cattacagca aacgtttcgg aaagacggtt cgccagatta acgccacccg
     3061 accaggcgac aatgccccct gaaacactgg ctccagcctg tcgataagta ctcgactgac
     3121 tataactgcc attcaccgtt gccaccggcg cgttccaggt taaattcgct ccagctgtcg
     3181 tttcatttcc ctgatgctga taactcaggt tgaccccata gttaaactga tcccggcttc
     3241 caacggttcc tgataatccc gtattatttg aggcaacccc ctgatcatca aacgtcgttg
     3301 agttagacat atatatttgc cgacgaggcg tcgtaacgtc atcaccccaa tcaaagggaa
     3361 tcgatataaa aatattaaaa cgtttctctt catgatgatt ctcgtcataa gcatggcttg
     3421 ccgcgagggt atagcttatc cttcgcaggt tgttggaata actcaactga taatccttgc
     3481 tgctgccgct acgcccccag taatctcgcc ataacgtact taaggacaca gaaccccagc
     3541 cttctggcaa tgactggctc atattggcag aaaagctatt tttacggcca aaatcgttct
     3601 ggtaataatc ggcaatgtca tagatatcgt tttcatcacg gcgataatta tctttattgt
     3661 ttgcccatac gtgatcatta aatgtccgat aatcacgcga cgaataacgc caggccgcca
     3721 gaccaaaacg tgtcgacgtt tggctcacaa atttgttgta ggcaatttga taactttgcc
     3781 cgtcaaacac atcgccgttg tcttgtatac tatgcgactt cgtggcatcg acggaaatgg
     3841 caccaatgcg tgtattccag ccagtcccca aagtgaacgc gtaataatta ttcgcgacca
     3901 tcgatccgcc atacagcgtc aataagttat taaaaccata ctgataaccc gcctgaacaa
     3961 aatcactttg tttgctcgcc ccttcaatat ggctacgacc tgccgcaaaa tcatatttcg
     4021 acacgccggg ttgcagcata ttcggcactg ctgcataagg caccagatag gtggttaccg
     4081 aaccgtccgc ctctttcacg ctgacatcaa gatctgctcc accaccggcc aactgcaaat
     4141 ctgtaatcgc aaacgggcca ggaggaacct ctttctgata aaccacaaaa ccattctgtt
     4201 caatagttac cagcgcgtta ctctgagcaa tcccctgcac ccgtggagta aaattttgtt
     4261 tcgagttagg caacatctgc atatcacgaa acaaccgcac accgctgaag cgaacagaat
     4321 caaaaatatc gcttgatgtg tacatatcac ccacgcgaag cgtgccgaga agttgggcaa
     4381 atccacgttc cagatacagg gtattgcttt tccacacccc tggattgcta tttgttttac
     4441 tgaaactggc atcagaatgc agttgccacc ccagtaagtt taacccgctg ttaaaacgta
     4501 catatgtact cttgctatta cccgacgctt tatagtcgct gtaatactga ctcacataat
     4561 aagaggtata aaacgcatta ataccccgct cccagttttc cggtggaaca tagccacttt
     4621 ccagttcttc cacccacgcc tgcgggacac tgaaatcgag acgaaaaact ccgatatccc
     4681 aggtatagct cccaccctga acaagttgct caaatgttaa acattgctta ccgctggcaa
     4741 agttatcggt attaatgcct aaccgcttga taatttccct tgataaacat gtttcttgcg
     4801 ggttgtcttt aacaataatc tcatatttcc cgcgccattg cttattgaca taaatatcga
     4861 tgtcatactg ccccggcaag ggttgattat catcaagacg aatatttgca acctgctggt
     4921 ctttcattcc acctatcatg aaatgggtat caaaggtttc ttcagctgca taagcttcaa
     4981 tgccgagcaa taacgctacg attgctgatg caagtggggt cattctcaac ataggcagct
     5041 cctgcaatta aattttgtca ctaatatagt tgccatggtc atcgataatg gtcagatgcc
     5101 agttattcgc attattactt ttgacattaa tactctgact ttctaagggg gcaatcataa
     5161 tagtttcata attgactttg acattattag ctttgacatc cgaaatcgtt acccaatttt
     5221 ctgagtcatt ttttatcacc aatccattgc cactgcgatt taccagtaac tttttaaatg
     5281 tcgctttatt taccggagca atacccgctg gccggtaaaa caacttaatt ctgttttgca
     5341 tcgcaaactt cagtgcattc ttaccttctt gttctggact atttggtgga atatccagaa
     5401 cattcagata aaaaatactc tctttattag tgggtagttt attcggcata attttaattt
     5461 ttacttgctg cccggaattt gcccctattt ttgccactgg tggcgttaac atgaaaggaa
     5521 cctgaatttt ttctggtggt aatgacgtat cgccatcatc aatccacgcc tgcagcagcg
     5581 aagaacggtt tccctggttc atcaactgca ccatcacttc tttattttct gccgggtaaa
     5641 taatgcgcgt cccgtagata acaataccgg catgtgcagg aaggaccata gaagaaatga
     5701 gtaaagctaa taatcctttc atcttaatgc ctcttaaatt aaaaggccgc catggggata
     5761 gcggccatgt ttattacctg gagaaacttg caattttatt tattaatcct gcgccacacg
     5821 cataaccaga gaagataaca catcacctga ttgcactgaa ttatctgtca gcttcaggta
     5881 ttttgcttta aacggaatag catattgacc ttccgctgtt ttataactct caatgttaaa
     5941 aggtgcagaa atatccagga catcactgtt atcacctaaa tagagatagt aacccacggt
     6001 atcgttgcta ctggctatcg ccttatcgtt gccggttatt ttgtcgtttg tgatcaacga
     6061 gagttttaca ccagcaactt ctttcgggca gctaattttt aacatgctgc tggttacacc
     6121 gactgcatca tcataggtag aatatttagc tttaccttca aagacagtgg ttggtacagt
     6181 tggcatatta atacttactg aatcgccacc tgcttcgaat ttacacggag attctgcaat
     6241 attcccttta atggttaatg ttccggtatc aacgtctgcg gcaaataccc cagcgctcat
     6301 aaaaaaagaa gaaaaaacag cggcagcaat aattgaacgt ttcataatat gctccttaat
     6361 aaaatctatt tttggttgtt aagagtctgt atctcttgga aacaaatttc tcacacccaa
     6421 aaagttgccg caatgtaaac acattgtttt attgatttaa agcaaattaa ccattgcaat
     6481 ataaacacaa caaatactta a
//
LOCUS       DAZKJR010000214         6478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKJR010000214 DAZKJR010000000
VERSION     DAZKJR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.215"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2769
                     /locus_tag="ACHOWS_004836"
     CDS             <1..2769
                     /locus_tag="ACHOWS_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFQ0050303.1"
                     /translation="RVDWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVILA
                     QKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFNRL
                     AAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDGGA
                     DISGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESRTF
                     SIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGTSA
                     VGGASGSTTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSSAL
                     TVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSGEY
                     DFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFHTL
                     TAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLVTA
                     PAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDAET
                     PNPVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVNSS
                     AVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNY
                     THVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALFDSGAY
                     IDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELVYG
                     AVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLL
                     ANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDNAV
                     NASFRYSF"
     gene            complement(3337..6009)
                     /gene="rcsD"
                     /locus_tag="ACHOWS_004837"
     CDS             complement(3337..6009)
                     /gene="rcsD"
                     /locus_tag="ACHOWS_004837"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFQ0050304.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         1589 a         1477 c         1793 g         1619 t
ORIGIN      
        1 cgtgttgact ggatggtgaa cggacttgcg ggagacgcac ttcataaaat tggtaaggga
       61 acactggtgg tggccggcag tggtgaaaat cctggcaccc tgaatacggg ggacggaacg
      121 gtcattctgg cacagaaggc tgatgctgca ggcagggtcc gggcattcag tgaagtcagt
      181 attgtcagcg gacgaccagt tgtggtgctg caggacagtc atcagattga aggggaccgc
      241 attcgctggg gataccgggg aggtacgctt gatatcaacg gaaatgacat agccttcaac
      301 cgcctggctg cggctgatga gggggcagta ctcacaagcc gggcgcgtcc tgccacagtc
      361 cgtctggatt tcagccagtc agggcagaag gccgtgatgt ggcatggtca ttttaccggt
      421 aacctcagcg tcctgaacaa cacgtcatct gcggtggatt ttatcatgga tggcggagcg
      481 gatatatccg gcagttttac acagcagggc gggggactgt atatccaggg gcatccggtt
      541 gttcatgctg tcagcagtga ggcggttgcg actgcattgc gaaagcaggg tgataattct
      601 gttctgacgc agccggtatc atttgctcag aaggactggg agagcagaac gttcagcatc
      661 ggtcaactga aactgaaggg ggcggcattc agcctttccc gcaatgcgac actgacggga
      721 gatattgatg ctgataatgc caccatggtg ctgggaagtg actcgctata tctggatatg
      781 aaggacggca cggggagcag cagtgcgccg gttaagggga cgtctgccgt gggcggtgca
      841 tccggtagca ctacatttcg cgggaatgtg aatatgcgtc attctgccct gacggtccgc
      901 gaccatttta ctggcagtat aacggcatcg gacagccgga tagctgtgag ttcagagaat
      961 gtgcgtctgg aagggaattc ccgcctgacc tcttctgcac ttactgtcag tgacggcggg
     1021 cgtttacatg tgaagggggg actggagaca gatggtggtg tgacactgga cagaggaacc
     1081 cttttggtgg acgggggaag tgtcaggaat gatgtatatg aacgtctgct ggcctggtca
     1141 gaggagcgtg gcgggctgaa cggaagcggt gagtatgatt ttatgacggg ggcagctggt
     1201 ctcctgcgga gttatgtcag agggagtgca ggtaatgtga atctgcagaa tgcggcctgg
     1261 atgatgacgg gaaactcatc cgtgaagcat cttgagtcat cgggaagtgc tctgtatttc
     1321 agcaggccgg gcggggagtt ccataccctg acggcaggca gtatggatat cagcgacagt
     1381 gttctggtga tgcggacgga ccttaaccac tcagaccagc ttcgggtgac agaatcgctg
     1441 cggggtaaga ataacctgtt gttggttgat tttactgagc gttctgacgg gcagaaggcg
     1501 ctgaatatcc ctctggtgac ggcgccggcc ggtaccggtg cggatgtgtt cagtgtgaaa
     1561 acgcgtgata ccggcttcag tcatatcaca cctgttgtca gggctgaaca ggggacgggc
     1621 ggcacggcct ggcagctgaa tgttgtgcag ccggaaactg ccgcagagcc tgtggcgacg
     1681 cggcaggatg cagagacgcc gaatcctgtg gaagctgtat caccgctccc ttcaccggtt
     1741 tctgctccgt catcggctcc ggaggcgtca gtgacggatg tactgaccgg agagaatgcg
     1801 ggggaatcag gtgagtaccg tgatgagacc cggtggctgc tgacaggata ccggagtacg
     1861 gttaatagct ccgcggtcag ggatgcgggg atgctgatgt caatggggca ccgtaacttc
     1921 attaatgagg tcaacaacct gaacaaacgt atgggtgacc tgcgggacat caacggcgag
     1981 gccggtgcat gggcccgtat catgagcgga accggctctg ccggcggtgg cttcagtgac
     2041 aactacacac acgttcaggt gggggcggat aaaaaacacg aactggacgg tctggacctc
     2101 ttcaccgggg tgaccatgac atataccgac agcaacgccg gcagcgacac cttcagtggt
     2161 aaaacgaagt cagtgggtgc gggtctgtat gcttcagccc tgtttgattc cggggcctat
     2221 atcgacctga ttggtaagta tgttcaccat gataatgagt atacggcgtc ctttgccgga
     2281 ctcggcacaa aagactacag ctctcattcc tggtatgccg gtgctgaagt gggttaccgc
     2341 tatcatgtca cagaggatgc ctggattgag ccacaggcgg aactggtcta tggtgctgtc
     2401 tccgggaaac agttcagctg gaaggaccag ggaatgaacc tcaccatgaa ggataaggac
     2461 tttaatccgc tgattggccg taccggtgtg gatgtgggta aatctttctc cggtaaggac
     2521 tggaaagtga cggcacgtgc tggtcttggc tatcagtttg acctgctggc gaacggagaa
     2581 acggtactgc gtgatgcatc cggagagaag cgtattaagg gcgagaaaga cggccgtatg
     2641 ctgatgtctg tgggtctgaa cgcagaggtc cgtgacaaca tccgttttgg tctggagttt
     2701 gagaaatcag cctttggtaa gtacaacgtt gataatgctg tcaacgccag cttccgttat
     2761 tcattctgac ggttgttact gaaggggatt atggcctgac ggctccctgt aaataaagac
     2821 aggcacagaa gattgtgagt tactcagtgt tccaccaaaa agtaaggtcg caactctgtg
     2881 cctgcttcgt aagtgtgcag tgagggccat atggttataa ctgaagcatc cgacgacgta
     2941 gtgtctacca gagtgtcaga tatgcaggaa aatatgagtt ccgacagaaa tgcagtaaaa
     3001 aaaggagtct cgcactcccc tccataaaac tccagggctg taagttgtat taaaaagctg
     3061 atttttcttt agcgatacag acctcaataa gtataacacc cttcagttac tgtggttatg
     3121 cacaggtgtt atttttaaca gtgtggataa acagtgtcga ttattcagat tggttatggg
     3181 gcatgtatgg gacattttta atcggcgaaa tacgtcgaag ttcgtcgata tggtaaacga
     3241 atcatctatc caacccttga aaaacggcgc tcctggacga tcttcgtcga tttttaaaat
     3301 gcaacaagta cgttgttcat gtaataggct accttgctac agcaagctct tgacataact
     3361 gtcaatgtcg ctgatgtatt tttctattcc tggaacatcc ttctcacgaa tcagatgttc
     3421 cagcgtttca cataactgct tgccgggtac cagatttagc atggcaaata cgcctttaag
     3481 acgatgagcc gtttgggcta acgcagcaaa gtcactggtc gctgcttcag tatacagcct
     3541 cttaacatca tccggtactg tgtctacaaa gagcgcataa tagccgctgg catggagttg
     3601 cgcattttca tctccgccca gaggcgattc tgtcacctct tcctgcgcca gttgcacttc
     3661 aattaattgt aagaccgctt cctgcatagc gttgctcata ttgaagttga cgcacaattg
     3721 accaggccca atttcccgta cgccagactc atcatcgctt aaaagcaagc cagaggcagt
     3781 aagattagac ggattatccg ttaaaaagat atcataatct tgactaatta atctttcatc
     3841 gggtgtgata caggttgcac cccaattttc taactggcga gtgacaatat tccgaatttc
     3901 tgccgaagta acatccacca ttacgcagac atcatccagt aaacgctctt cttcctcttc
     3961 aacttccggg tcagctgcga gcattttgat atgcacagag tagcgagtac caagcccatc
     4021 ccgcgttttg atgtttaaat gaccgcccag tttacgcgcc agttgatcgc tcagccagaa
     4081 tgccagcggg tccgccttgc catagcgatc gttttgggtc tggttgataa acgggaagtg
     4141 caaattatcc atttcatgaa tgcttacgcc ttctccagtg tccagaatac ggaacgtcag
     4201 gcggtcttcg gaggactcat cctgatcaac ctcaagggtg atttttccca attgcgttga
     4261 ggtcacggca tattgcatca gtaacagcaa aatacgtcgt aaggcatcgc gatcgccgcg
     4321 gcgcatgtcg tgtgctttca gatgattgtt aatcagcagt tgcagacctt tacgcttgat
     4381 ggcaggcaac actgaaggca caacttcatc aattaaatcc tgcacggaga acagcaccgt
     4441 ctcacttttc cagctatcgt ccgcaagcat gttcgctaac tgtatttcat cgaccagccg
     4501 caccagcaca tccgcctgat tcgtcagttg tttgccttcc ggggcgttga gtttagccgc
     4561 gctctccgcc agggactgtg cgggttcttt cagcgcatcg ccaatgtttt tcataaaggt
     4621 catccgcccc tgctggtttt tctcatacag acgctgcgcc tgcttgagtt tcttgtttac
     4681 cagcacttcg cgatcctgat cgcgaataat gaaaatttgt gtgcgcggcg caacctggct
     4741 gcggaacatg cggatctcat acagctcgtt attgatcgtc gcctgaataa tcccctgatg
     4801 ctgttccgcc atggtggtga tgttttgcag attcaaatgc ggcagcaaat gatcggcaat
     4861 tttgttactt atgacagtgc ggttcgattc ctgatcgtga accagcaggc cgagcggcag
     4921 cagtgagact atctcttcat tgattgcccg taaaatgcgc aattcgttat tgaccgccgt
     4981 gttgggtaga ctttctgtac tgcgactgga gaaatggcga aatgtggtat agccaaataa
     5041 cgccagcgcc agcaaaccga tgttcagcag cagtggcagc agaatgtttt gcaacgtatc
     5101 cagcaataag gtgccataag gaacctgcca gaccaggcgc atatcggtag agttgagtgc
     5161 cgaggagatt tcaatcttcg tactgttaaa gtggatactg acgctatccg tcccttcttt
     5221 ctcattatca ttgtctcccg ttgccgtcgc gtctggctca aggcggaaac tgtccagcgg
     5281 catacccggt gggatcaaat cattaatcgg cagatcaaaa gccacaaccg ttgccagatg
     5341 tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga ccgttctgcc aggccaggcg
     5401 gcgcatgtta gaaaagcttt cgcgttcatc gagggcgttg gcctgttgca acatctctgc
     5461 acgacgtgaa tcaacaatgt cgctgacggt cgattcttta aatccggagg tgagatcttt
     5521 gaggggcagg gttgagatca gcaccagact gttatcctga ccattcaggt aatacatcga
     5581 ccacggtaca ttttctgcgc cccacaatgt atccagataa gtggacatcc gttgagtcat
     5641 ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct tccgttttgc ggcgtggttt
     5701 ctccagatag tagacatcct gtttcaggcg cgtctcttgt aaaccttcgc cggaggaggg
     5761 ggtggtcgtc gcggcaatgt tgtcgtagat ctgccaggtc acgtaacgcc aggtatcgac
     5821 gcgcttttgg atagcgtggg taatgtcgac aatctggtaa cttttatctt tcagccaggc
     5881 gttaacggcg ctttgtacca ttacacccat cgtcaccagt aacacaatga tcaacagtaa
     5941 aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg gtcgtggccg ttgtctcttt
     6001 ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa gtgatagggc agtataaagg
     6061 gtacagtgtg aaattccaga ctcttaccgg attgatgatt ctctattctc ccgtgatatt
     6121 tatcatcctg cattggggga aatagatgta caagcgccat tttgctgtac aaataaccta
     6181 caaaaagcct aacgaaatta attgcaaata aacgaagatt gctggaaatt atgctgatgt
     6241 taattatttg tgaaatagtt aacaagcgtt atagtttttc tatggtagca cagaataatg
     6301 aaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg aaataggggt
     6361 aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat agcggaagat
     6421 attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata attaagaa
//
LOCUS       DAZKJR010000215         6450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKJR010000215 DAZKJR010000000
VERSION     DAZKJR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.216"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..261
                     /locus_tag="ACHOWS_004838"
     CDS             <1..261
                     /locus_tag="ACHOWS_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFQ0050305.1"
                     /translation="EKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGI
                     RHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            463..852
                     /locus_tag="ACHOWS_004839"
                     /pseudo
     CDS             463..852
                     /locus_tag="ACHOWS_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(1051..2175)
                     /gene="yhhJ"
                     /locus_tag="ACHOWS_004840"
     CDS             complement(1051..2175)
                     /gene="yhhJ"
                     /locus_tag="ACHOWS_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0050306.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(2175..4910)
                     /gene="rbbA"
                     /locus_tag="ACHOWS_004841"
     CDS             complement(2175..4910)
                     /gene="rbbA"
                     /locus_tag="ACHOWS_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFQ0050307.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYHPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     ACLQEAAGQSNEAEAPPVIHDTTHAARQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMVPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4907..5974)
                     /gene="yhiI"
                     /locus_tag="ACHOWS_004842"
     CDS             complement(4907..5974)
                     /gene="yhiI"
                     /locus_tag="ACHOWS_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0050308.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(6037..6150)
                     /gene="yhiY"
                     /locus_tag="ACHOWS_004843"
     CDS             complement(6037..6150)
                     /gene="yhiY"
                     /locus_tag="ACHOWS_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFQ0050309.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
BASE COUNT         1561 a         1773 c         1647 g         1469 t
ORIGIN      
        1 gagaagttcg ccacagcaat ccgaaaccac tggcacgtgg agaataagct gcactggcgt
       61 ctggacgtgg taatgaatga agacgactgc aaaataagaa gaggaaatgc agcagaatta
      121 ttttcaggga tacggcacat tgctattaat attttgacga atgataaggt attcaaggca
      181 gggttaagac gtaagatgcg aaaagcagcc atggacagaa actatctcgc gtcagtcctt
      241 gcggggagcg ggctttcgta atcttgccct gatcagacca ccgttgatca ccatatccat
      301 gagtttatga ataaagggca gggaaagatc acgcaacgct gcttttttgt actaaataat
      361 tcgcactaaa tattcaataa ttgagatatt ccttattacc taaagctgtt ttccattgct
      421 tatacatgat caaatactcc ttacataatt aaggagaaca aaatggaact taaaaaattg
      481 atggaacata tttctattat ccccgattac agacaagcct ggaaagtgga gcataaattg
      541 tcagatattc tactgttgac tatttgtgcc gttatttctg gtgcagaagg ttgggaagat
      601 atagaggatt ttggggaaac tcatctcgat tttttgaagc aatatggtga ttttgaaaat
      661 ggtattcctg ttcacgatac cattgccaga gttgtatcct gtatcagtcc tgaaaaattt
      721 cacgagtgct ttattaactg gatgcgtgac tgccattctt cagatgataa agacgtcatt
      781 gtaattgatg gaaaaacgct ccggcactct tatgacaaga gtcgccgcag gggagcgatt
      841 catgtcattt ctcgcgtcag tccttgcggg gagcgggctt tcgtaatctt gccctgaata
      901 ccggactgag tataaaaaag ccggttaact gaaactgtcc aggttttggt ggtcagttca
      961 taaacgcctt atccggccta aaaaaaacta aaattcaata aattgcacca atgcgtagac
     1021 cctataagct tacgcatcgg gcaactgtaa ttacgccatt gtcccaattg tcttcctgaa
     1081 tctcagcagt gcgatggtga aaaacgcgcc gccaattgcc atcagtgtca ggaactgcgg
     1141 ccagacgatt tcgaatccgg caccacggta gagaatggcc tgcgcgaggc taacaaagtg
     1201 tgtcgtcggc atggtcagca taatgtcctg caccatctgc ggcatacttt cgcgcggcgt
     1261 ggaaccaccg gaaagcattt gcagcggcag cagcaccaga atcaccagca gccccagttg
     1321 cggcattgaa cgcgctatcg tccccataaa aataccgatt gacgtggtgg caaacagact
     1381 gagcgccacg cccagcataa acagcgggat cgagccttct atcggtacgc ccagcacgcc
     1441 ttttaccatc agcaccagcg ataatcccga caccaccagc accaccagcc ccatcgacca
     1501 gacctttgcc atcatgatct caaacggcgt tatcggcatc accagtaagt gttccaccgt
     1561 gccgtgttca cgctcgcgga tcagcgccga tccggtcaat acaatcgcca gcatggtaat
     1621 gttgttgatg atcgccatca ccccgccaaa ccacgcggga tcaaggttcg ggttaaagcg
     1681 catccgtgtt tccagcgata ccaacggttc gctgttatca cggtagcgcg cgacaaagct
     1741 gttcacttca ccgttgataa tattctggat atacccattg ccggtaaatg cctggctcat
     1801 gcgcgtggca tcgacgttca cctgaatatc cggctggcgt ccggcgagga catcacgctg
     1861 aaaattaggc ggaatattta tcgcgaaggt ataacgtccg gcgtccagcc cggcatccat
     1921 ctcatcggcg gtgatcatct ccggtggcaa aaaccacggg cgatagaagc tgttaacgat
     1981 ccggttcgat aactgcgatt gatccatatc ggcaatggcg atcggcgcga ggttcaacga
     2041 tcctggcgta acggtcgctg acgaatacac cgacaccgta aacgagaaga caatcagcgt
     2101 cagcatcgct ttatcaccga gcagactgcg caactctttg atacccagat taaaaatatt
     2161 acgtaaatgg cgcatcatcc ctcctgtttt ttcagcagca ggatgcttaa gcccatcacc
     2221 agcggtatgg ctatcagcaa cgggataaaa agttgccaca aatcagtcag atccagcgct
     2281 ttcgagaacg ttccgcgggc gatagtcaga aaatgactgg tcgggtaaac ctcgccgatc
     2341 caacgtcctg gaccttccag cgaagctacc ggatcgatca tcccggaaaa ctgtgtcgcc
     2401 gggatcaacg tgataattgc cgtaccaaaa atggcggcaa tctggctttt cataaaggtg
     2461 gagatcagca gccccattcc ggtggcgatg atgatataca gcagcgccgc cagggtgaga
     2521 gtcaggaaac tgcctttatg cggtacgcca aatacaaata ccgacagggc gcagagcagg
     2581 aaaaagttca gcatccccag cgcgatgtat ggcagctgtt tgccaagcaa aaattcgcta
     2641 cgggtggtgg gggtcacgta aaggttgata atcgacccta gctctttttc ccgcaccacg
     2701 ctaagggcgc ttagcattga cgggaccatc atcagcagaa gcgggatcac cgccggaaca
     2761 atcgccggca ggctttttac gtccgggtta tagcgatagc gcgtctcaat attcatcagc
     2821 ccgctttggc tggcgggtgt ggactgtcgg ctcgccacat cctgtagcca gctctggtgc
     2881 atggcctgca cgtagccttt taccgtttcg gcgcggctcg gcatcgcacc gtcgatccag
     2941 acaccgagtt ccacgggcgt accacgcgcg atatcgcgcc cgaaattagg agggatctca
     3001 attgccaccg tgatatcgcc cgcacgcatc cgacgatcaa gctcgtcata actggtgagc
     3061 ggcggctgtt cgataaagta acgggaaccg gagagattga gcgtccacgc ctggctactg
     3121 acggtttgat cgcggtcaag caccgcaaag cgcaggtttt ccacatccat actgatgccg
     3181 taacccatta tcagcatcag gatcaccgtt cccatcagcg ccagcgtcga acgtaccgga
     3241 tcgcgtcgca gttccagcgc ttcgcggcgg ctgtagctaa acagacggcg caggctaaat
     3301 ccctgacgcg ccgcgtgggt ggtgtcgtgt atcacgggcg gcgcttcggc ttcgttgctc
     3361 tgccctgccg cttcctgcaa acaggcgata aatgcctctt ccagacttgc ggctccgcgt
     3421 ttctcaacca gttcctgcgg tgtcccgctg gcgagcactt ttccggcgtg catcagtgag
     3481 atacggtcgc aacgttccgc ttcgttcata aagtgggtgg agatgaagat agtcactttg
     3541 tcctggcgcg agagatcgac catcaactgc cagaacatat ccctcgccac cggatcgacg
     3601 ccagaagtag gctcatcgag gattaacatc tccgggcgat gaatcaccgc caccgccagc
     3661 gaaagccgct ggcgaatgcc gagcggcaat gactccggca gaacatcttc aacgtcgttg
     3721 agcttaaaac gctcgctcat ttcagccact cgcgcgggaa tttccgcttc cgggatgtga
     3781 aacaaacggg catgtaactc aaggttttgc cgcacggtga gttcgttata gagcgaaaac
     3841 gcctgcgaca tatagcctac ccgacggcgg gtatcgatat cttttggatc aaccggttgc
     3901 ccaaacagcc acgcctcacc ttcgctggcg ggcagcagtc cggtgagcat tttcatggtg
     3961 gtggatttac cgcagccgtt cgaaccgagg aatccaaaaa tctccccgcg tggaatgcgg
     4021 aaattgacgt gatcaacggc aacgaaggaa ccaaaacgca tggtcagatc gcgcgcttcg
     4081 atggcaatct ctgcgttttc agggtgatac ggtgggatca ctaccgcctg atgcgcctgg
     4141 cgttgcgctt gcggtaacag atttataaat gcttcttcca gcgtggcgct ttgcgtttgc
     4201 tgccgcagct cttcggcgct gccggttgcc agcacttctc cggcattcat cgctaccagc
     4261 cagtcgaagc gttcggcctc ttccatgtag gcggtggcga ccagcacgct catattgctc
     4321 tgccgctggc gaatgctgtc gatcaaatcc cagaactggg cgcgggagag cgggtcaacc
     4381 cccgttgttg gctcatcaag aatcaacagt tccgggtcgt ggattaacgc acagcacagc
     4441 ccaagttttt gcttcatccc gccggagagt ttccctgccg gacgatcacg aaacggtgct
     4501 aacccggtgc tggtcagcag ctcattgatt cgcacttccc gctccgcttt gtcgtgaccg
     4561 aacaggcgag cgaaaaaatc gacgttttca tacaccgaca aggtgtggta gaggtttttg
     4621 cccagcccct gcggcatcca ggcgatgcgc gggcaaacgt cgcggcgatg cttcgggtcg
     4681 cgcatatcgc cgcccagcac catcacgctg ccctgctcaa tgacgcgggc accggaaatc
     4741 aacgacaaca agctcgattt accgacacca tccggtccaa tcagcccgac catgcaacgg
     4801 gccggaatat cgagtgtgat attgttcagc gcaacggttt ttccataatg ctggctcacg
     4861 cccgccagtt gcgcgacagg cgggacggga accagttcca gatgcgtcat tgcggcaacc
     4921 tcaccaccag gtcgtcaggc cacggaagtt cttcattcac ccgcacccac gctacgcccg
     4981 gcaatccggt tttgacatat tccagatgct gctggagtaa ttccggtggg atacgcgctt
     5041 tgacacggaa catcaatttc agccgctcat cgctggtttc gacggttttt ggcgtgaact
     5101 gggcgacgct ggcgacaaaa ctgatggtcg cgggaatacg cagatctggt gcagcatcaa
     5161 gaatcaaacg tgcttcaccg cccagtttca gcgtgcccgc ctgttcggtt ggcagaaaga
     5221 aggtcatata gacgtcgctg agatcgacca tattcagcac ccgaccgcct gccgccagca
     5281 cttcgcctgg ctcggcaacc cgatactgca cgcgtccgtc acgcggggct ttcagttcgc
     5341 tgtcatcgat atctgcggca atgcgccgtt cagtggcttg tgccgcttcg acgcgagttt
     5401 gcgcctgaat gatattggtg cgtgccgcct ctatagcggc tttagaagca gaaacctgag
     5461 cttttgccga ttccagcgca gctcgggcgc tctcagcggc ggcgcggtcg tcatccaact
     5521 gttgcgcaga aattgccccc cgttgtgcca gtgagcggga acgcgtatga cgttttgcta
     5581 cggagtccag ttctgcctgg cgttgattaa ccagcgactg tgcggcacga gtttcgcttt
     5641 ggcgttgctc cagcaaagcc tgcgcggcgg caacggcact ttgtgcttct ttgatttgcg
     5701 cgatggcttc cagtcgctgt tcctgcaaca cgcgagtatc catcttcgcc agcacttcac
     5761 cttctcgaac aaactggcct tctttcacca gaatggtgtc gatacgcccg gcaattttgc
     5821 tggcgatatc cacttccgtc gcttcaatgc gcccattgct gacagcaaag ccttccggca
     5881 cacccgccgg gcgcaacagc caccacgcca cgacggcagc caccgccagt gccccgacaa
     5941 cccaccacgc cagatggcgc ttactcttat ccataatcga tccgccataa tccctgtaag
     6001 caatcgacgc gtgttacaac tgccaccgaa cgatgttcag cggattcttc catccgggat
     6061 aagaaggaaa tggcaaatac gaccagcgca cagagcattg agcgccaatg gggaaggctt
     6121 agtgaaaacg ggtagtaatg ttgtcatcat cagcattcct ggccgtaaat gaaacacgca
     6181 tactcataag tgaaatcaat taaatactaa cagttatatt catgttgtct atttgattgc
     6241 catcaacttg tgatacatcg cgatgttctt ccgttcgctg gtgttttttc caaaaatatt
     6301 ctgtacaggt tcacagattc tgttgcacag gtttccgtaa cagggaaacc tgatgactat
     6361 cacatagcgt tattatcgcc tgatgtgcga ataaataccg aatataaaat aatttattct
     6421 gaaaagatga tagatgataa aggctcgtgg
//
LOCUS       DAZKJR010000216         6152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKJR010000216 DAZKJR010000000
VERSION     DAZKJR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.217"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1309
                     /gene="alsA"
                     /locus_tag="ACHOWS_004844"
     CDS             <1..1309
                     /gene="alsA"
                     /locus_tag="ACHOWS_004844"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFQ0050310.1"
                     /translation="LAAQLGIGIIYQELSVIDELTVLENLYIGRHLTKKICGVNIIDW
                     REMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTLMLDAKVIIMDEPTSSLT
                     NKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVMKDGSSVCSGMVSDVSND
                     DIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDRKKVRDISFSVCRGEILG
                     FAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLDAVKKGMAYITESRRDNG
                     FFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQRELLALKCHSVNQNITE
                     LSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYKVMRQLADDGKVILMVSS
                     ELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALPHE"
     gene            1288..2268
                     /gene="alsC"
                     /locus_tag="ACHOWS_004845"
     CDS             1288..2268
                     /gene="alsC"
                     /locus_tag="ACHOWS_004845"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFQ0050311.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            2279..2974
                     /gene="alsE"
                     /locus_tag="ACHOWS_004846"
     CDS             2279..2974
                     /gene="alsE"
                     /locus_tag="ACHOWS_004846"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFQ0050312.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDNHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            2958..3887
                     /gene="alsK"
                     /locus_tag="ACHOWS_004847"
     CDS             2958..3887
                     /gene="alsK"
                     /locus_tag="ACHOWS_004847"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFQ0050313.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            4160..6145
                     /gene="yjcS"
                     /locus_tag="ACHOWS_004848"
     CDS             4160..6145
                     /gene="yjcS"
                     /locus_tag="ACHOWS_004848"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFQ0050314.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETI
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAKEANKQGDYRWSAELLKQVIAANPGDQVAKNLQADNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMV
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         1551 a         1541 c         1578 g         1482 t
ORIGIN      
        1 attagcggca caactcggca tcgggattat ttatcaagaa ctcagcgtta ttgatgaatt
       61 aaccgtactg gaaaatttat atattggtcg tcatctgacg aaaaaaatct gtggcgtcaa
      121 tattatcgac tggcgagaaa tgcgtgtccg cgccgccatg atgttattac gcgtgggctt
      181 gaaagttgat ttagatgaga aagtggcgaa tttatctatc agccacaagc agatgctgga
      241 aattgccaaa acgctgatgc tcgacgccaa agtcatcatc atggatgaac ccacctcctc
      301 actcactaat aaagaggtgg actatttgtt tctgatcatg aatcagttgc gtaaggaggg
      361 tacggccatc gtctatatct cgcataaatt ggcggaaatt cgccgtattt gcgaccgcta
      421 tacggtgatg aaagacggca gcagcgtttg cagcggcatg gtgagcgatg tgtcaaatga
      481 cgatatcgtc cgtctgatgg taggtcgcga actgcaaaac cgttttaacg cgatgaagga
      541 gaatgtcagc aaccttgcgc acgatacggt ttttgaggtg cgcaacgtca ccagccgtga
      601 cagaaaaaag gtccgggata tctcatttag cgtctgccgg ggagaaatat taggctttgc
      661 cggactggtc ggttccggac gtactgaact gatgaactgc ctgtttggcg ttgataaacg
      721 cgctggcgga gaaatccgtc ttaatggcaa agatatctct ccacgttctc ccctggatgc
      781 cgtgaaaaaa gggatggctt acatcactga aagccgccgg gataacggct ttttccccaa
      841 cttttctatc gctcagaaca tagctatcag ccgcagtctg aaagacggcg gctataaagg
      901 cgcgatgggg ttgtttcatg aagttgacga gcaacgtacc gctgaagccc agcgcgaact
      961 gctggcgctg aaatgtcatt cggtaaacca gaacatcact gaactctccg gcggtaacca
     1021 gcagaaagtc ctgatctcca aatggctgtg ctgtcacccg gaagtgatca ttttcgatga
     1081 acctacccgt ggcatcgacg ttggcgcgaa agccgaaatt tacaaagtga tgcgccaact
     1141 ggcggacgac ggaaaagtca tcctgatggt gtcatctgaa ctacctgaaa ttatcaccgt
     1201 ctgcgaccgc atcgccgtgt tctgcgaagg acgactgacg caaatcctga ccaatcgcga
     1261 tgacatgagc gaagaggaga ttatggcatg ggctttacca cacgagtaaa aagcgaagcg
     1321 agcgagaaga aaccgttcaa ctttgcgctg ttctgggata aatacggtac tttttttatc
     1381 ctggcgatca tcgtcgccat ttttggttcg ctgtcgccag aatattttct gaccaccaat
     1441 aatattaccc agatttttgt gcaaagctcc gtgacggtac tgatcggcat gggggaattc
     1501 ttcgctatcc tggtcgccgg tatcgacctt tcggttggcg cgattctggc gctttccggt
     1561 atggtgaccg ctaaactgat gttggcaggt attgatccgt ttctcgcggc gctaattggc
     1621 ggtgtactgg ttggcggcgc actgggggcg atcaacggct gcctggtcaa ctggacggga
     1681 ctacacccgt tcattatcac ccttggcacc aacgctattt tccgtgggat cacgctggtg
     1741 atctccgatg ccaactcggt atacggcttc tcatttgact tcgtgaactt ctttgccgcc
     1801 agcgtaattg ggatacctgt ccccgttatc ttctcgctaa ttgtcgcgct catcctttgg
     1861 tttctgacaa cgcgtatgcg gctcgggcgc aacatctacg cactgggcgg caacaaaaac
     1921 tcggcgttct attccgggat tgacgtgaaa ttccacatcc tggtggtgtt tatcatctcc
     1981 ggtgtttgtg caggtctggc aggcgtcgtc tcaactgcac gactcggtgc cgcagaaccg
     2041 cttgccggta tgggttttga aacctatgcc attgccagcg ccatcattgg cggcaccagt
     2101 ttcttcggcg gcaaggggcg cattttctct gtggtgattg gcgggttgat catcggcacc
     2161 atcaacaacg gtctgaatat tttgcaggta caaacctatt accaactggt ggtgatgggc
     2221 ggattaatta tcgcggctgt cgcccttgac cgtcttatca gtaagtaagg aattgaacat
     2281 gaaaatctcc ccctcgttaa tgtgtatgga tctgctgaaa tttaaagaac agatcgaatt
     2341 tatcgacaac catgccgatt actttcatat cgatatcatg gacggccact ttgtgccgaa
     2401 tctgacgctc tcaccgttct tcgtaagcca ggtaaaaaaa ctggcgagta aaccgctcga
     2461 ctgccatctg atggtaacgc gtccgcagga ttacattgct caactggcgc gtgcgggagc
     2521 tgatttcatc actctgcatc cggaaaccat caacggccaa gcgttccgcc tgattgatga
     2581 aatccgccgt catggcatga aagtggggct gattcttaat ccggaaacgc cggttgaggc
     2641 catgaaatac tatatccata aggctgataa aattacggtc atgactgtcg atcccggttt
     2701 tgccggacaa ccgttcattc ctgaaatgct ggataaactt gccgaactga aggcatggcg
     2761 tgaacgagaa ggtctggagt acgaaattga ggtggacggt tcctgcaacc aggcaactta
     2821 cgaaaaacta atggcggcag gggcggatgt ctttatcgtc ggcacttccg gcctatttaa
     2881 tcatgcggaa aatatcgacg aagcatggag aattatgacc gcgcagattc tggctgcaaa
     2941 aagcgaggta cagcctcatg caaaaacagc ataacgtcgt agcgggcgtg gatatggggg
     3001 caacgcatat ccgcttttgt ctgcggacag cagaaggtga aacgctacac tgcgaaaaaa
     3061 agcggaccgc agaagttatt gctcccgacc tggtgtcggg tatcggcgaa atgattgacg
     3121 agcaactcag gcgctttaac gctcgctgtc atggtctggt gatgggattt ccggcgctgg
     3181 tcagtaaaga taaacgcacc attatttcta cgcctaacct gccgttaaca gcggcagatt
     3241 tatatgatct cgccgataag ctcgaaaata cgctgaattg tccggttgag ttttcccgcg
     3301 acgttaacct gcaactctcc tgggacgtag tagaaaatcg ccttacgcaa caactggttc
     3361 tggcagccta tctcggtacg gggatggggt tcgcagtgtg gatgaacggt gcgccgtgga
     3421 cgggtgcgca cggtgtggca ggcgaactgg gccatatccc cctgggagat atgacccaac
     3481 actgcgcgtg tggcaattct gggtgcctgg aaaccaattg ctctggaatg gcgctaagac
     3541 gctggtacga acaacagccc cgaaattacc cattgagcga tcttttcgtc catgcggaaa
     3601 acgccccttt cgtccagagt ctgcttgaaa acgcggcacg agccattgcc accagcatta
     3661 atctgttcga tcccgatgcg gtgattctgg gtggtggcgt gatggatatg cccgccttcc
     3721 cacgcgagac tctcattgcc atgacccaaa agtacctgcg ccgtccactg ccgtatcagg
     3781 ttgtgcgctt tattgccgcc tcatcttctg actttaatgg cgctcagggt gcagcaatat
     3841 tggcgcatca acgttttttg ccacagtcct gtgctaaagt cccatgagta aagcgttggc
     3901 acagtagcgc aatgtcccgc tgtgccaacg acaggtacaa tatatataat aattacataa
     3961 aacagcccat attattccca gcaatgatag ttctactgta cttttccctc ttcagcacaa
     4021 atgcctaata aaatggcgcg ttcagctcac acttctactt attaaagtcg caatttccca
     4081 attatgataa cgccagcttt aaatgacgta ttttgcatgg attacatatt tacgacataa
     4141 atgataaatg gagatttata tgaataactc tcggttattc cgtttgagca ggattgttat
     4201 tgcgttaact gccgccagca gcatgatggt aaataccgct aatgcaacag atgaagcgaa
     4261 agccgccact caatataccc aacaggttaa tcagaattac gccaaatcat taccgtttag
     4321 cgatcgtcag gattttgacg atgctcagcg tggatttatc gctccgctgc tggatgaagg
     4381 tattctgcgt gatgcgaacg gtaaaattta ctaccgcgcg aatgattaca aatttgatat
     4441 taatgccgct gcgccggaaa ccataaaccc cagcctgtgg cgtcagtcgc agatcaacgg
     4501 catttcaggc ctgttcaaag tcaccgataa aatgtatcag gtgcgcggcc aggatatctc
     4561 taacattacg ttcgttgagg gcgagaaagg cattattgtt atcgacccgc tggtgacgcc
     4621 gcctgcagca aaagccgcac ttgatcttta cttccagcat cgtccgcaaa aaccgattgt
     4681 tgccgttatc tacactcaca gccacgccga ccactatggc ggcgtgaaag gtattatctc
     4741 tgaagccgat gttaaatccg gcaaagtgca ggtgattgct ccggcaggct ttatggacga
     4801 agccatcagc gaaaacgtgc tggcgggtaa catcatgagc cgccgtgcgc tctactctta
     4861 cggtctgtta ctgccgcaca acgcgcaagg caacgtgggc aacggcctgg gcgtgacgct
     4921 ggcaacgggc gacccgagca ttattgcacc gactaaaact atcgtcagaa ctggcgagaa
     4981 gatgattatc gacggcctgg aatttgactt cctgatgact ccgggtagcg aagcgccagc
     5041 cgaaatgcac ttctatattc cggccctgaa agccctgtgt accgccgaga acgccacgca
     5101 taccctgcac aacttctaca ctctgcgcgg cgcgaaaacc cgcgatacca gcaagtggac
     5161 cgagtacctg aacgaaacgc tggatatgtg gggtaacgac gcggaagtac tgtttatgcc
     5221 gcacacctgg ccggtctggg gcaataagca tatcaatgat tatattggta aatatcgcga
     5281 taccatcaag tacattcacg accagaccct gcacctggcg aaccagggct acaccatgaa
     5341 tgaaatcggc gacatgatta agctgccgcc tgcgcttgcc aataactggg ccagccgcgg
     5401 ctattacggt tctgtcagcc acaacgcccg cgcggtgtat aacttctatc ttggctatta
     5461 cgacggtaac ccggctaacc tgcatccgta tggtcaggtg gagatgggta aacgttacgt
     5521 gcaagctctg ggcggttctg cccgcgtcat caacctggcg aaagaagcga acaagcaagg
     5581 tgattaccgc tggtcggcag aactgctgaa acaggtgatt gccgccaacc ctggtgacca
     5641 ggtcgcgaag aatctgcaag cggataactt tgaacagctg ggctatcagg ccgagtccgc
     5701 cacatggcgc ggtttctacc tgaccggcgc gaaagagctg cgcgaagggg tacataagtt
     5761 cagccacggc accaccggtt ccccggacac cattcgcgga atgtcggtcg aaatgctgtt
     5821 cgactttatg tccgttcgcc tcgatagtgc gaaagccgcg ggtaaaaata tcagcctgaa
     5881 cttcaatatg agcaacggcg ataacctcaa cctgacgctg aacgatagcg tgcttaacta
     5941 ccgtaaaacg ctgcaacctg aagccaacgc ctctttctac atcagccgtg aagatctgca
     6001 tgccgtgctg accggacaag tcaaaatggt ggatctggta aaagcgaaga aagccaaaat
     6061 tattggcaat ggcgcgaaac tggaagaaat tattgcctgt ctggataatt tcgatttgtg
     6121 ggtgaatatc gtaaccccaa attaatgcta ct
//
LOCUS       DAZKJR010000217         6089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKJR010000217 DAZKJR010000000
VERSION     DAZKJR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.218"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..1625
                     /locus_tag="ACHOWS_004849"
                     /pseudo
     CDS             74..1625
                     /locus_tag="ACHOWS_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
     gene            1651..2103
                     /locus_tag="ACHOWS_004850"
     CDS             1651..2103
                     /locus_tag="ACHOWS_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFQ0050315.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTFIPERLHKT"
     gene            2256..3530
                     /locus_tag="ACHOWS_004851"
     CDS             2256..3530
                     /locus_tag="ACHOWS_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0050316.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3523..5624
                     /gene="mchF"
                     /locus_tag="ACHOWS_004852"
                     /pseudo
     CDS             3523..5624
                     /gene="mchF"
                     /locus_tag="ACHOWS_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
     gene            complement(5685..6008)
                     /locus_tag="ACHOWS_004853"
     CDS             complement(5685..6008)
                     /locus_tag="ACHOWS_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050317.1"
                     /translation="MRNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPSADI
                     AGSAIGGYIGGVIADGSNRTITIPSNGINYNPSIGLGSFNPNYNGGLLNSGFNSSTSS
                     SSSGS"
BASE COUNT         1755 a         1160 c         1450 g         1724 t
ORIGIN      
        1 gaagctgtca ggacacttac ttgctctttt atgaacactc ttttatgaaa attgaaacgg
       61 agaatactgc cagatgagtc atcagtgttc actttctgaa ctgaatgaaa acctggtgcc
      121 tttcactgcc aggcagatca aggcctcatt aatctggtgt gcagaggatg tcagaaatcc
      181 agacgagctg caaaatgcct gcagttatat tatcgatcct ggcagtacag cttctgccaa
      241 agtgttccat gcagagcgct atggtggcag tggtattcag cgtaatggag gtggtgcacg
      301 ttgtgggttt gatggtaact accaggttaa aggaatagga agtaatccgt tggttggcga
      361 aggtactgac gaacgtcatt ctaatggtgc actcggcgct gttcatgcaa tattatgagg
      421 ctttgtgggg agaagtactg gctcaaatat taccttatag tgctgtgcgg gttcgggcgg
      481 ttttacttac agatctctat actgaaaagg catttgagcg ctccggtagg aaatcacgaa
      541 gagccctgtt ggtacgtgag cctgttgttc gtccggcgca ttttgaacgg gcaccttact
      601 tccaagtaaa accggagtat tccagtctgc taattcacga tgcctgtcgg gttagatctg
      661 tgatccacaa gctgccagga tatctacctg taccaccgga agaaattgat gctgaagcac
      721 gaactgatcc ccggatttat tgcattgagg ggttatgtga actggcacgt cgtgaggcct
      781 ggcaaatggc attttgtcga acacgtttcc tgagattgac aacttctcct tctaatattg
      841 caatggatgg cagattaatg gattttaacg gactcagttg tctgtttccg ggagattccc
      901 cagctgattt tgggtataaa ctaagattag ctgaactggc aaaagaaccg atggtactta
      961 tgcaagggct gtctgatctc tgcttgtata tcggaaaata tatgtttgac cctgacttca
     1021 ctcttgcagc ccgtttgaag gttgaggaga tatttcagaa aactttccat gaagcatgtt
     1081 attactgtta tctagaactg ttgggtattc ctggagaatt tataacacaa aaagagatac
     1141 ctgatatatt gaaagaactg gttaacagtt ttgttgcatt actcaatcaa tactgcgaga
     1201 aatcacatgc ccaagatatt gtcaatcagg atggttcacc attgcaaaag ttggttgtga
     1261 cgctaatcca tcataggcat aatcaaaagc aggcactgaa tagtagcatc aagaatgatg
     1321 tttatttcac cgttgcacaa cagtgttttt cccaagccat ccactggctg acgcaaggca
     1381 gtaccagacg tcagataaat gcctcattac tcctgaaaga aattgaacat cataccatga
     1441 aaaggctgca acccagggaa gagctgagga aagaaaatat gtgcgaaaaa attgccatcc
     1501 tgctggataa tcatggcgat gatccccttt ttttacaaga agcaatttct gatatgaaaa
     1561 attttatgct taagttttcc agagatgcat ttggatatct tgaaccgata agaaacacag
     1621 tgtaaaaaat atataaggga aatagtaatc atgtcttata taagggaaac catcagagga
     1681 aaagatgaat ggactgttta tgaacagatc ggttttgcgg tcagttgtat gctctacaat
     1741 cgtaattaca gtctgtatcc ggtgttaacc attcaatact ggactgaata tgcgatacag
     1801 cataatcaga ttaaattcct gtttgattca cgaggttttc cactggcgta tattacctgg
     1861 gcatatcttg aggctgatac agaagcgcgc ctgctcaggg atccagaatt caggttgcat
     1921 ccgtctgaat ggaatgaaga tggaaggatt tggattctgg atttctgttg taaaccaggc
     1981 tttggtcgaa aagttattga ctatctcata cagcttcagc catggggaga aggagaagta
     2041 cgatggttaa gcaggcgaaa gaaaattgtg acattcatcc ctgagcggct gcataaaacg
     2101 tagtacctct gaagatacag agataattgt aaattacggg gtaaatgcat cgctgatact
     2161 attttgacag gactctgtat tttctggctt tgattatagc atttcgctta accagtgaag
     2221 gtattacagg aaaagtgtga gtaagaggag ctattttgtt tcgtcaggat gctttagaaa
     2281 acagaaaaat gaagtggcag ggacgggcaa tattacttcc cggaatacca ctatggttaa
     2341 tcatgctggg aagcatagtg tttattacgg catttctgat gttcattatt gttggtacct
     2401 atagccgccg tgttaatgtc agtggtgagg tcacaacctg gccaagagct gtcaatatat
     2461 attcaggtgt acagggattt gttgtcaggc agtttgttca tgaaggacag ttgataaaaa
     2521 aaggggaccc tgtttatctg attgacatca gtaaaagtac acgtagtggt attgtcactg
     2581 ataatcatcg tcgggatata gaaaaccagc tggttcgtgt ggacaacatt atttcccgtc
     2641 tggaagaaag taaaaaaata acgctagata ccctggaaaa acaacgcctg caatacacag
     2701 atgcgtttcg tcgctcatca gatattatac agcgtgcaga ggaagggata aaaataatga
     2761 aaaacaatat ggagaattac agaaactatc aggcaaaagg gctgattaat aaagatcagt
     2821 taactaacca ggtggcatta tattatcagc aacaaaacaa tcttctcagc ctgagcggac
     2881 agaacgaaca gaatgctctg cagataacca ctctggagag tcagattcag actcaggctg
     2941 cagattttga taaccgtatc taccagatgg aactgcaacg gtacgagtta cagaaagaac
     3001 tggttaacac tgatgtggag ggcgaaatca ttatccgggc gttgactgac gggaaagttg
     3061 actccctgag tgtcactgtc gggcaaatgg tcaataccgg agacagcctt ctgcaggtta
     3121 ttcctgagaa cattgaaaac tattatctta ttctctgggt cccgaatgat gctgttcctt
     3181 atatttcggc tggtgacaaa gtgaatattc gttatgaagc ctttccggca gaaaaatttg
     3241 ggcagttctc tgctacggtt aaaactatat ccaggactcc tgcgtcaaca caggaaatgt
     3301 tgacctataa gggagcacca cagaatacgc cgggcgactc tgttccctgg tataaagtca
     3361 ttgcgatgcc tgaaaagcag attatcagat atgacgaaaa atacctccct ctggaaaatg
     3421 gaatgaaagc cgaaagtaca ctatttctgg aaaaaaggcg tatttaccag tggatgcttt
     3481 ctcctttcta tgacatgaaa cacagtgcaa caggaccgct caatgactaa cgggaatttc
     3541 agacaaatta taaatcagct tgatatgcgc tggcgacgtc gtgttccggt tattcatcag
     3601 acggagaccg ctgaatgtgg actggcctgc ctggcaatga tatgcggtca ttttggtaag
     3661 aatattgacc tgatatctct tcgccggaag tttaatctct cggcccgtgg agcaaacctt
     3721 gcaggaatca atggaatagc ggagcagctg gggatggtca cccgggctct ttcactggag
     3781 ctggatgaac ttggtgccct caaaatgccg tgtattctcc actgggattt cagtcacttc
     3841 gtcgtgctgg tcagcgtaaa gcgtaaccgt tatgtactgc atgatccggc cagaggcaga
     3901 agatatctcg gtcgggagga aatgagccgg tattttacgg gcattgcact tgaggtctgg
     3961 cccggaagtg aattcctggc ggaaacccag cagacccgca taagtctccg ttcactgatt
     4021 aacagtattt acggtattaa aagaacactg gcgaaaattt tctgtctgtc agttgtaatt
     4081 gaagcaatca atctggtaat gccggtggga actcagctgg ttatggatca tgcgattccg
     4141 gcgggggaca gagggctgct gacgcttatt tctgctggcc tgatgttctt tatattgctc
     4201 agggccgcgg tgagtatgct gcgtgcatgg tcctcactgg ttatgagcac gctcatcaat
     4261 atacagtggc agtcgggtct gtttaaccat cttctcagac tgccgctggc gttttttgaa
     4321 cgccgtaaat taggtgatat ccagtcgcgt tttggctccc ttgacacttt gagggccacc
     4381 tttaccacct gtgttgttgg ggcaatcatg gacagtatta tggttgtggg tgtttttgtg
     4441 atgatgctgt tatatggagg atatcttacc tggatagtgc tcggttttac catggtttac
     4501 gttcttattc gtctggtgac atacggctat taccggcaaa tatcggaaga aactcttgtc
     4561 agggggcccg ggccagctcc tattttatgg aaagcctgta tggtattgcc acggtaaaaa
     4621 tccagggtat ggctgggatt cggggaacac actggcttaa cctgaaaata gatgcgatca
     4681 attcaggtat taagttaacc aggatggatt tgctcttcgg ggggataaat acttttgttg
     4741 ccgcctgtga tcaggtggcg attttatggc tgggtgcaag ccttgtgatc gataatcaga
     4801 tgacaatagg aatgtttgtg gcatttggtt cttttcgtgg gcagttttcg gatcgggttg
     4861 cttcgctgac cagttttctt cttcaactga gaataatgag tctgcataat gagctcattg
     4921 cagatattgc actacatgaa aaggaagaaa agaaaccgga aattgaaatc gttgctgaca
     4981 tgagcccggt ttcactggaa accactgatt taagctaccg gtatgacagc cagtcagcac
     5041 aggtattcag tggtctgaat ttgtctgtgg ctccgggaga aagtgtggct ataactggtg
     5101 cctccggtgc cggaaaaacc acattaatga aagtattatg tggactgttt gaaccagata
     5161 gtggaaaagt actggttaat ggcacggata tacgtcaact tggaataaat aattatcacc
     5221 gtatgatagc ctgtgttatg caggacgacc gactattttc aggatcaatt cgtgaaaata
     5281 tctgtgggtt tgcagaagaa acagacgacg aatggatgac agaatgtgcc aaagcaagtc
     5341 atattcatga tgtgataatg aaaatgccaa tggggtatga aacgttaata ggtgaactgg
     5401 gggaaggtct ttccggcggt caaaaacagc gtatattcat tgcccgagct ttataccgga
     5461 aacctggaat attatttatg gatgaggcta caagttctct tgatacagaa agtgaacgtt
     5521 tcgtgaatgc tgctataaaa aagatgaata tcacccgggt gattattgca catagagaga
     5581 caactttaag aacagttgat cggattttta gaattacaaa ttaggataat atatcactaa
     5641 aagcagcatt gctgcataat aagctatgct gctttagaaa ttaatcaact tccactacta
     5701 gaagaggatg ttgatgagtt aaatcctgag tttaataaac ctccattgta gtttggatta
     5761 aaacttccaa gtcctatact cggattatag ttaataccgt ttgatggtat tgttattgtc
     5821 ctatttgaac catcagcaat aactcctcca atatatcctc caattgcact tcctgctata
     5881 tccgcactag ggatttttgt tgctttacct aaaaaaccac cagccgcagt acctattgca
     5941 acggatgcat ctttatctaa tcctgcacca tgtattaaag caagttcatc ggttgttata
     6001 tttctcatgt tatttccttt ttttattaaa gaaagataaa acaaataaaa acagtacact
     6061 cgcaagtaac gaaagaatta ttttcttta
//
LOCUS       DAZKJR010000218         6086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKJR010000218 DAZKJR010000000
VERSION     DAZKJR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.219"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..228
                     /locus_tag="ACHOWS_004854"
     CDS             <1..228
                     /locus_tag="ACHOWS_004854"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0050318.1"
                     /translation="LQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPY
                     FISVFREYFGITPHQMMINQSGFIESRIAIK"
     gene            1141..5232
                     /locus_tag="ACHOWS_004855"
     CDS             1141..5232
                     /locus_tag="ACHOWS_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFQ0050319.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTDRRLMTVSLVLSVT
                     LSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDNNGNAVGTLDVPMIDFS
                     SVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNNYTVVKNNKHSGLDFSLHRFS
                     KLITEAAPADINIFGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIPGTFLTGGTVGT
                     PWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDSLSEKWSLAGVTLHNYG
                     VNGYRNNWLLLPEDYIRNIIVADFDPIISFNKNSKEHMSWTYDAAKGVGRIQQDDQQF
                     VMHGNLNGNLNAGKNLYFTGENGIIDLKDNVNQGAGYLQFADDYTVTTSNDSSWSGGG
                     IIVNYGTTVKWGINGVFGDDLHKVGDGTLIINGTGKNEGGLKIGAGTVILEQKAKNND
                     STAFSSINISGGNSRVKLSGDNQIIPDNVSWGFRGGYLDINGKDTEFSRLQAVDYGAA
                     IINSSIDKSLLTLNLSPLKKDEIAVSVKVLDMNAIFQGGHGTAGDLYKTTFYGPTQYY
                     LLKKPKFGSVLMGALKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNM
                     DIVIPELTGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREF
                     SLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKS
                     AAAGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLN
                     IHKGGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSS
                     VLKARDQASVVGDIISDKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAI
                     HAPSASATVNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAY
                     AMRTDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSE
                     QAIGFSNVTPVITAIDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNL
                     NKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFT
                     VTHTDSSASADAFKGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGT
                     RDYSTHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDY
                     NPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSR
                     MLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            5765..6085
                     /locus_tag="ACHOWS_004856"
                     /pseudo
     CDS             5765..6085
                     /locus_tag="ACHOWS_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1785 a         1257 c         1430 g         1614 t
ORIGIN      
        1 cttcaggagg aaaaagtttc tttctcccaa atcctgctgg atatacgcat gcataacgcc
       61 cgacaactta tttccagtaa actgtcagtg aatcaaatcg caatacgttg cggttatgcc
      121 agcacaccgt attttatcag cgttttcaga gaatattttg gtataacacc tcatcagatg
      181 atgattaatc agtccggatt tattgagagc agaatcgcaa ttaagtaaag tatgtcagtc
      241 agcgcataat aaaagtattc ttatgcttca ttatcagttt ctgtttatca ctccggagca
      301 ctgtagtaac tacggacccg gaaaacagat ggtaatgagt tgactgactg atgatattgt
      361 ggcgacattt ttacggatgc gagcaaagaa tgtattggtc cgtttgatta tgcagtgatt
      421 aacaaaactc cgggaagatg aatgagtata cctctcctgc aatccgtttc ctgagaaaac
      481 aggtggtaac acaatattct taatatgtta ctgtttcacc acacaaccag gtttacggtt
      541 gtgtggtgaa ctatagtaat cccgggtaac aatccggagc tgttcctttt atactcttca
      601 gactgtgtac tcatccacgc catctgtttt tcagtctcag tgtgtgaata ataaattaca
      661 ctttgaagtg aacgccagtc aggaccatgt cgtatttccg gatggatgag catatatcag
      721 aaactacaat tactacagat aacgcttttc agtaattctg tcataacttg ccggaatgta
      781 atgttttgtg acatggttct tattttttat acacaggcag gttttgtcgg gatggaatgg
      841 ataatgaact cttatcatta taaggcggta acctcttttt tatgtgatta actttcatta
      901 ttaccaattc tgtttatcga ttcagtactg gaaatacaga aaaattcagt gcaggtgtat
      961 aaatcatcac catgcgaaat acataaaata tattaatgtt tgtttttcgc aggaaaaatc
     1021 ttaagtgtgg acaggtatct ctgatgccat ttaaaataga ttcagagtga gtgcccagca
     1081 ctcactcttt ccggacagat aatacatttc aataccatat aattatataa caggaattat
     1141 atgaataaaa tatacgcgct taaatatagc tcccttactg gtgggcttat agctgtgtca
     1201 gaattaagta agaaggtcac aggaaaaacc gatagaagat taatgacggt ttccctggta
     1261 ttatcagtga ctctttctgc tttaccgggt aaagcatcaa cggtcagtgc agaaatacca
     1321 tatcagactt ttcgtgactt tgctgaaaat aaaggtgtgt ttacccccgg agtgacagga
     1381 attgaaataa aagataataa tggaaatgca gttggtacgc ttgatgtgcc aatgattgac
     1441 ttcagcagcg tgtcacggcg gggctcactg accctgttgt cgcagggata tggtgtatca
     1501 gcaaaacacg gagaccttgg tgatgtaaac aatgccagtt ttggctatga taaaaataat
     1561 tatactgtag taaagaacaa caaacattcg ggccttgatt tttctctgca tcggttcagc
     1621 aaactcatca cagaggctgc acctgctgat ataaatatat tcggacaact tagtgattcg
     1681 tcacagtata ctgcttttta cagagcaggc gctggcacac agtatattaa agaacgctca
     1741 ggtaaacaga ctcatatccc gggaacattt ttaactggtg gaacagtagg cactccatgg
     1801 tattcaggaa ataatttaat atcaagctct ccaggtgaca cctataataa atcacaggga
     1861 ccattagcca gttatgggca gatgggtgac agcgggtcgc cattatttgc ttatgattct
     1921 ctcagtgaaa aatggtctct ggctggagtc accctgcata actatggggt gaacggttac
     1981 agaaataact ggttgttatt acctgaggat tatatcagaa atattattgt agccgatttc
     2041 gatcccatta tttcatttaa taaaaacagt aaagaacata tgtcatggac atatgatgca
     2101 gccaaaggcg tgggccgtat tcagcaggat gaccaacagt ttgtgatgca tggtaacctg
     2161 aatggtaatc tgaatgcagg taaaaatctt tattttacag gcgaaaatgg cattattgat
     2221 ttaaaagata atgttaatca gggtgccgga tatcttcagt ttgctgatga ttatacagta
     2281 acaacgagca atgacagttc atggtccgga ggaggcatta ttgtcaatta tggtacaact
     2341 gtaaaatggg gtataaacgg ggttttcgga gatgaccttc ataaagttgg ggacggaacg
     2401 cttattatta atggaaccgg gaaaaatgaa gggggactaa agattggggc tggtactgta
     2461 atccttgaac aaaaagcgaa aaacaacgat tcaacggctt tttcttccat taacatcagt
     2521 gggggaaact cgcgcgttaa actttcagga gataatcaga ttatacctga taatgtttcc
     2581 tggggattcc gggggggata tttagatata aacggaaaag acactgagtt ctcccggtta
     2641 caggctgttg attatggggc tgcaattatt aacagcagca tagacaagtc attattgact
     2701 ttaaatctgt caccattgaa aaaagatgaa atagctgtaa gcgtgaaggt gttagatatg
     2761 aacgccatct ttcagggggg acacgggaca gccggagatt tgtataaaac aactttttac
     2821 ggcccgacac agtattatct gctgaaaaaa cctaaatttg gcagtgtgct gatgggggca
     2881 ctgaaaaaca ccagcgaatg gcaatttgca ggaacagacc tgaatcaggc cgttgatatg
     2941 gcaaagaata ataaactgac atcaagtgca caggcttcat atctttatca cggtaagctc
     3001 ctggggaata tggatattgt cattccggag cttacaggca atgatatttt aacgcttgat
     3061 ggctctgtca gtatatcagg agatatgtca aaacaggacg gtgctcttat cttccaggga
     3121 cacccggtta ttcatgcagg gcaaactgtt tctgcatcgc agagtgactg ggagaacagg
     3181 gagttctcac tcaacaatct gaatcttaat aatgcggact tcagtctgtc ccgtaatgca
     3241 tttatgaacg ggaatatcag ggccgttaac cagagcactg ttattatcgg cggagataca
     3301 gtctttactg ataaaaatga cggaacaggt aatgatgtca tcagtgttga agggaaatct
     3361 gctgccgcag gaacatcctc ctatacaggg catatcactc tggagcaaaa atcagcactg
     3421 gatatccgcg ataattttcg tggcggggtt acgtctgaag acagtcatat caatgtttct
     3481 tcatcttcag tcctgttctc agatgcatcg tcatttataa acagctccct gaatattcat
     3541 aaaggaggtg cgctgaccgc tcagggaggg ctgtttacaa gtggaagcat tgatattggt
     3601 gacgcttccc ttctgcttac cggtacacca gtgaattcag atgatgctgc ttttttaccg
     3661 accatcaata tggctgatgg cggatttaaa ctgatgtctg attcatcagt actgaaagcc
     3721 agagaccagg catctgttgt tggtgatatt atttctgata aacaggccac aatcagcttc
     3781 ggaactgaat caggtaaaga gggcatattg tctgagaagg catcccgggg actcgcggta
     3841 ggattactga gtggttttaa tacggcatac cgcggtgcaa ttcatgcccc gtcagcatct
     3901 gccactgtga acaacacctg gtggcaattg acaggagact cctcacttcg ctcgttaaaa
     3961 aataccggaa gcatgacata ttttacagga agtgcagcga ataaagcatt ccatacactg
     4021 acggttgatg agctgacgac gaatggcact gcgtatgcca tgcgtacgga cctgaaaaat
     4081 gcggataagc tggtagtaaa ccaaaagctg tcaggtaagg acaatattct gctggttgat
     4141 tttctgaaca aacccaccgg agaaaaactg gatattgaac tggtgagtgc accggggaac
     4201 agcagtaagg atgtttttaa aggaagtgaa caggcaatag gtttcagcaa tgtcacgcct
     4261 gttattacag ctatagacgc cggagataaa acaacatgga acctgaccgg gtacaggatg
     4321 gcagaaaatc ctgccgcaac ccaaagtgcc tcaggccttg catctgtggg gtacaaatca
     4381 tttttgagtg aggtcaacaa cctgaataaa cgtatgggtg acctgcgtga catcaatggt
     4441 gaagctggcg catgggcacg tatcatgagc ggaaccggct ctgccggtgg tggtttcagt
     4501 gacaaccaca cacatgttca ggtcggtgtc gacaaaaaac atgagctgga cggactggat
     4561 ttgtttaccg gcttcactgt cacacatact gacagcagtg cctctgctga tgctttcaaa
     4621 ggtaaaacaa aatctgtggg ggccggactc tatgcttccg ccatgtttga ttccggtgcc
     4681 tatatcgacc tgattggtaa gtatgttcat catgataatg agtacaccgc aacctttgcc
     4741 ggactcggaa cccgtgatta cagtacgcat tcatggtatg ccggtgctga agcaggctac
     4801 cgctgtcatg tcactgagga tacctggatt gagccacagg cagaactggt ttacggtgct
     4861 gtatccggta aacagtttgc atggaaggac caggggatgc atctgtccat gaaggacagg
     4921 gactacaatc cgctgattgg tcgtaccggt gtggatgtgg gtaaatcctt ctcaggtaag
     4981 gactggaaag tgacagcccg tgccggtctg ggctaccagt tcgacctgct ggctaacggc
     5041 gaaaccgtat tgcgggacgc atcaggtgaa aaacgtatca aaggtgaaaa agacagccgt
     5101 atgctgatgt ccgttggcct gaatgcagaa atcagggaca acgtccgctt tggactggag
     5161 tttgagaaat ccgcctttgg taagtacaac gttgataatg ctgtcaacgc taacttccgt
     5221 tactcgttct gatactccgt attcaaaagg gcctgagggc cctttatgct caggacctgc
     5281 cgcccgggta tacatttccg gagtatcagt cgatttgttt acttaaccgc catgaccaaa
     5341 gccggcagta atgtcggctt tttacgtcca tatattccac tcatccggaa ctgtgacaca
     5401 gaacctgaat acaggattga tcaccggtgc ccacaaatgg cacattttca gtaagattct
     5461 gaatgttaca ttcccccttc atgtgtgaca cagctgtcac agagaacgaa acggaaagca
     5521 catgaaaaac ataaaaactc acaccggact ccttatccac aaagaacaaa cacggcgcgt
     5581 tcgtcttcat gaaacaccgg ccgcctggtg ccacacacgc cgggaatgct acagcaaaac
     5641 gaccggtcgt cgctgcggca gccctgacag tctcagcagg ctgattctga gcagcattca
     5701 gcccatagaa tgagataacc cgtatgaatg atgacagacg tctacggtac ccgatgtatg
     5761 gaacatttgc tttctggcta acggcatgcc cgacacatgg ttttacaccg atgacgcctc
     5821 cgtcacaaaa gagtgggggc gggaatattg tgacaatgtg gaagtgttcc ctctgtgcta
     5881 tatgcatgac ggtcccgttt tccgggggcg ctatattgat gagctcagcg ccgcacggaa
     5941 cagaatgctc catatgcacc acatactgcc ggaatgaacc cggtacagag aaaattttca
     6001 tgacgcagaa tgtatccgtg aaattcacga agcgcccctg caggctcccg gcattctgca
     6061 aaaatacaaa cgcacaaaat cataag
//
LOCUS       DAZKJR010000219         6082 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKJR010000219 DAZKJR010000000
VERSION     DAZKJR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.220"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..1256
                     /locus_tag="ACHOWS_004857"
     CDS             54..1256
                     /locus_tag="ACHOWS_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0050320.1"
                     /translation="MILVCVILWGIYVAVRALINLNERFIDAVSSPAGIIGLFFGLLT
                     VFAILLRFFIYRRLRKKTAAFEQAVSELVQRERGFNETVNAVIARGIRQEKEQLARRR
                     EEFHTARQKASRAMQRIVDSAWKFKAKTLLAGVTINNWQSKYDQLRKEREAYAAVSEK
                     IAFLNLEDNSDWESVRQQFLDKVALLEKAQEEKEYQAELKRQIREEKERQDELDRRQR
                     EAEEEERRLAEQQKLIEEALRAAEGAHREELEKQRLELEQKIQEAHAQYERAKSMAQL
                     TKQGHVYIISNIGSFGEDVFKIGMTRRLEPMDRVKELSGASVPFDFDVHAMISCDDAP
                     ALEKTLHDSLEKYRINRINLRKEFFRVKLEKIINEVERHHGQMEYVADPAALQYLQSL
                     EYAENEAA"
     gene            1491..3236
                     /locus_tag="ACHOWS_004858"
     CDS             1491..3236
                     /locus_tag="ACHOWS_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0050321.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLMYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            3302..3607
                     /locus_tag="ACHOWS_004859"
     CDS             3302..3607
                     /locus_tag="ACHOWS_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050322.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            3662..3976
                     /locus_tag="ACHOWS_004860"
                     /pseudo
     CDS             3662..3976
                     /locus_tag="ACHOWS_004860"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            4301..4450
                     /locus_tag="ACHOWS_004861"
                     /pseudo
     CDS             4301..4450
                     /locus_tag="ACHOWS_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4625..5059
                     /locus_tag="ACHOWS_004862"
                     /pseudo
     CDS             4625..5059
                     /locus_tag="ACHOWS_004862"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            5202..5291
                     /locus_tag="ACHOWS_004863"
                     /pseudo
     CDS             5202..5291
                     /locus_tag="ACHOWS_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5696..>6082
                     /locus_tag="ACHOWS_004864"
     CDS             5696..>6082
                     /locus_tag="ACHOWS_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HFQ0050323.1"
                     /translation="MAKMIPAFGPIDNNSYGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIGYGFIF"
BASE COUNT         1614 a         1320 c         1639 g         1509 t
ORIGIN      
        1 taaaggaaag tcatggcagg aagaaaaaag atgataatgc agcgggattt gtcctgattt
       61 tagtttgcgt tattctctgg gggatttatg tggcggtcag ggcgctgata aatcttaatg
      121 agcgctttat tgatgcagta tcgagtccgg caggtattat tggtttgttt tttggtttgc
      181 ttactgtatt tgcaatttta cttcgttttt ttatttaccg gagactcaga aagaaaacag
      241 cagcctttga gcaggccgtt tcagagctgg ttcagagaga gcggggtttt aatgaaactg
      301 tcaatgccgt gatagcccgg ggaatacgac aggaaaagga gcaactggcc agacgtcgtg
      361 aggaatttca taccgccaga cagaaggcca gccgtgcaat gcaacggatt gttgattccg
      421 cctggaaatt taaggcgaag actctgctgg ctggtgtaac gattaataac tggcaaagca
      481 aatatgacca gcttcgtaag gaaagagagg cttacgctgc tgtcagtgag aaaatagcct
      541 ttcttaacct ggaagataat tctgactggg agagtgtcag gcaacagttt ctggacaaag
      601 tggcattgct ggaaaaagcg caggaagaga aggaatatca ggcagagctt aagcgtcaga
      661 tacgtgaaga aaaagagcgt caggatgaac ttgaccggcg ccagcgggaa gcggaagagg
      721 aagaacggcg tcttgctgag cagcagaaac tgattgagga ggcgctgcgc gcagcagaag
      781 gggctcacag ggaggagtta gagaaacaac gcctggagct ggaacagaaa attcaggaag
      841 cacatgcgca gtatgaacgt gctaaatcca tggctcagct tacgaaacag gggcatgttt
      901 atatcatttc gaatattggt tcgtttggtg aggatgtctt taaaattggc atgacccgac
      961 ggctggaacc catggacagg gtcaaagagt taagtggtgc ttctgttccg tttgattttg
     1021 atgttcacgc catgatttcc tgtgatgatg cgcctgcgct ggaaaaaaca cttcatgaca
     1081 gtctggaaaa ataccgcatt aacaggatta acctgcgtaa agaatttttc cgcgtgaagc
     1141 ttgagaaaat cattaatgaa gtggagcgtc accacgggca gatggaatat gtggctgacc
     1201 cggcggcgct gcagtacctg cagagtctgg aatacgcaga aaatgaagca gcctgacagt
     1261 cagtgttctg attttacccc ggccggatat aacagaaggg atatctgcag ataccctgca
     1321 gcatggttca cagtattctg gtcatgttaa tgattctgtt ttttatttct gaataaagcg
     1381 acacccacga tataacatcc gtggatgtcg gtggtgatta ttttttgttc acagattaag
     1441 gtgcgtttat ggcgaaactg ataaatgtat cggcctccac tgaggagaga atgacgcctg
     1501 gggagaggag ggtggcttca cgactggaga gctttctgaa tgatgactgc cttgtctggt
     1561 acgatattcc ggtgggacgg aaaaaccggc atccggattt tgtcattatt gacccggaga
     1621 acggactggt atttctggag gtcaaggact ggacggtttc cacgctgcgc caggtaaatc
     1681 aggagcaggt cacgctggaa accgacggtc ttctgaaatc agaaatcaat ccgctggtac
     1741 aggttcgtcg ttatgcctgt gatacggtta atgccttacc tgcaaacccg tgcttacggc
     1801 agaatgacgg tcagtataag ggacggctga acctggcctg ggcgtatggt gtggtgttta
     1861 cccgcattac ccgtcagcaa ctgaagacac tggcagggaa tgatgagaat gcggtggaaa
     1921 aaatttttcc ttcagcacag acaatctgcc aggatgaaat gacgcagtct gttttacctg
     1981 aggtattcca gcaaaaaatt gccgggatgt ttaccacggg attcagaacc cgggtgacac
     2041 cccggatgag agatattctg cgcgcccatc tgtttcctga agtgaccgtg aaacagaact
     2101 cacaaatcaa aatcatggat attcagcagg agattctggc cagaaatatt ggtgacgggc
     2161 atcgtgtcat tcatggtgtg gccggttccg gaaaaaccat gattcttttg ttccgttgcc
     2221 tttacctggc tgaaaccaca cccggaaaga ttctggtgtt gtgctacaac attaccctgg
     2281 ccagctatct gcgggaatgc attgaagcca gagggttggc gtcacgggtt acggtatccc
     2341 attttcacag ctggtgtgcc tcaatggtaa aacgccacgg tattaaggtg acggccgaca
     2401 gaaaagaata tcctgataaa tgcttcagtg cactggaaga ggccgtgaac agcggaaaaa
     2461 tcacccgaac cggatatgat gctgtactgg tggatgaggg gcatgatttt gacagtcgct
     2521 ggctggccct gattgcccgt ctgtttgata acgcatcccg ttcactgctc ctgatgtacg
     2581 atgacgccca gtccatttac cggcgggagc gggccctgaa cttttctctg gccagtgtgg
     2641 ggattcaggc acagggacgc acttctgtcc tgccggtcaa ttatcgtaat ccgaaacgca
     2701 ttctgcactt tgcttatgca ttctcgcgcg agtattttga aaaacatcag aaccgtgaaa
     2761 taccgctcgt tcagccacag gcgggcggtg aagagggtac ggagccggaa atcctgcgtt
     2821 gtgcttctga acgtgatgaa gccagacaag ttgcagactg gctggagaag cgccatgccc
     2881 tgtgtggtca ctggagtgat atggctgtgc tgtgtccgac agagcgttca gcgcaacaat
     2941 tgcaggacgt tatgacacta cgcggggtgc cttttgccac ctgttttgac agggaaggca
     3001 agaagacata ctcgcgacgt aaggatgtgg tgcatttact gatgtaccag agcagcaagg
     3061 ggctggagtt cccgtatgtg gcggttatca atgcgtcttt cgttcactct ggtgcagcgg
     3121 atgagtctga agtgattccg gtgctttatg tggcgttcac ccgggcaacc cgtgaattac
     3181 tggtgacctg ttaccgtgaa aacagtatca gtcgtcacct tgaggatttt gcataatacc
     3241 ggattatttc tttattccgg actttcatca tactgttatt atcaggttta tttaattcgc
     3301 catgaacccg ttatttaatg atattcagat gcgtcttttt tatctgaacc actctccgta
     3361 ctcctggcac tggaatgtca gattcagacc ccaggaagct gtctatattg gcagcgatac
     3421 ctgtcatatc accattacct gtaatcagtc cggttttcat ttaaccaggg acggacaacg
     3481 gctgttcact gaacgttata tcagaaacct taatgagctg ttaccggttc tgaaacggcg
     3541 gtgggatgtg acaccggcaa tcatccgtgc cgtggaatac ctttcccggg tcccggtatt
     3601 acactgacat tcttttcgtc actgttaatc atgctttaat acagtaacac cgaatctgcc
     3661 gatgacttct gaagtgaaac tgaaaaccgg tggtgacccc aggtcattac cggattatgc
     3721 tgccctgcgt gatgaaatca gcaaactgac ccacccggca cgtccggatg tggactggcg
     3781 ttacgtggaa acgctctgcc tgcgtctgta tgaacacaat ggcgtggaat tacagacggc
     3841 atcctggtac accatcgccc ggatgcatac agcagggtta agtggactga atgaagggct
     3901 ggctctgatt gtggcgctga cccggcatca ctggtcagtg atgtggccgc tgaataccca
     3961 cgcccggctg gaaattatta ccggtctgtt caaccgtctg caaaaaacgc ttcgggccat
     4021 gcctccggac gaccgtgaca acctgcccct gctgtatcag accgaaacat tcctgaaggc
     4081 actgagtgac acacttgccc ggcacgaact taaacagggc agtaaagtgg ctttaccgga
     4141 agccatggtg aagggatata tcacccgcct ggaaaatcag cctgtgcagg gcgaagcgtc
     4201 gtctccggta acgcttccgg ctcaggcact ccggtctgat gctccggatg tgcaggaaca
     4261 tcagaccctg ccgcactccc gtctggtgta tgttgtttct gaaacaaaag cagagcccac
     4321 accctccctt cagaaatccc cgcccatgtt tctgaagccg tttttggctg gtgtctgtgc
     4381 ggcattgctg gctgtcagcg tggccattcc gggatggcag tttctgacac agccatcacc
     4441 ggcggagcag caactcaggg cgctgctgac acccccaccg gatgtgcttt cggcagacca
     4501 gatagcacag ctcagcagaa cacgctcact gttaaatcat acgtcagagt ggatacccct
     4561 ttccggtcaa cagataacgc gactgacaga attacccctg gcatacaacc tgcagcgcag
     4621 tgcgcagctg ctgcagcaac tgagggtact tttccccgat aatccccggg tacaggaaat
     4681 ggtggataac tggcagaaga gtgtaaggag ccgtgcgctc ccggaagagg cgatgacggg
     4741 ctggaacgaa ggcatgaccc gcctacagca gctggctgag cgcttgaacc gtctggatga
     4801 acagcgggga aaatacatga cagtcagtga actgaaaaca gaggtgtttg gcatcatgca
     4861 ggcttttaac cggcatatcc cggcagaaga gcagttacgt cgctataatg aagtccgcaa
     4921 ccagaatggc agtgaacaac agcaaaaact gacgcaggat gccctgacgg aactgttgaa
     4981 ccgctactgg tttctgagac atggagatgc aggtaagaag acgataaata ataaacagga
     5041 taaccagatt aatatctgac agattttatt gccattattt atccagaagc gatgatgcca
     5101 ttaatgcaaa atgacatctg aattgatcac agattggacc aatctgtgat caaatagaac
     5161 agaatacgca gatgataaag cggctttccc tcgaagattc acaacgtcat ctggcagaat
     5221 atgggtgaga gttgcaggct gatgcgtaca aagcttacga tgcgttgtat gaaactggcc
     5281 gggtgaaaga tgtcatccgt acggtgcgcg ccagactaaa caggcatcat gatggaaaag
     5341 tcgggtatca ttcacaggca tatttgtcag aatggtagcg tcagcgggtt ttgattacac
     5401 cagttttgaa gctcatactt cctatgaggc actgcagaaa acgaaagtgt cgtttaggcg
     5461 ctgggggagg ggttaaaaaa gtaacggcag aagtcaccct gaagttgagg cagtcaacta
     5521 ctgttagctg gctgctgcat gagagtgtgg agggagattg tatatattgg ttagtcagag
     5581 tctgcttcgg aataagtatg agtaattttt aaaatgggcg gaagttattt ttaattactg
     5641 ggctatgtaa caaatgcaga ttcctgtcag aaacatgtta taaggaaata gtaatatggc
     5701 caaaatgatt ccggcatttg gacccattga taataatagt tatggggaaa aaacagtcta
     5761 tacactgtta aaagaaggtc tgtccgatga tttcacagtg attcatagtc tcccgtggtt
     5821 aagtgcggcg acacgaaatt tagggatgaa attacctccg agtggagaaa ttgattttct
     5881 tgttctccat cctgtatatg gtgttttggc gttagaggta aagagtggaa tatatcgcgt
     5941 agaaggtact gtttttgtac atataaaaac aaaaaaacat gtagatatag tgaagcaaac
     6001 acgtaacaat gtccatggtt tagcccgttg gttaggggga gcaacaacgt tacggttgcg
     6061 tattggttat ggttttattt tt
//
LOCUS       DAZKJR010000220         6022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKJR010000220 DAZKJR010000000
VERSION     DAZKJR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.221"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            126..773
                     /gene="fucA"
                     /locus_tag="ACHOWS_004865"
     CDS             126..773
                     /gene="fucA"
                     /locus_tag="ACHOWS_004865"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFQ0050324.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            801..1949
                     /gene="fucO"
                     /locus_tag="ACHOWS_004866"
     CDS             801..1949
                     /gene="fucO"
                     /locus_tag="ACHOWS_004866"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0050325.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2004..2759)
                     /gene="xni"
                     /locus_tag="ACHOWS_004867"
     CDS             complement(2004..2759)
                     /gene="xni"
                     /locus_tag="ACHOWS_004867"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFQ0050326.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFKSLEGIYENLNAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2870..4237)
                     /gene="sdaB"
                     /locus_tag="ACHOWS_004868"
     CDS             complement(2870..4237)
                     /gene="sdaB"
                     /locus_tag="ACHOWS_004868"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFQ0050327.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4295..5584)
                     /gene="sdaC"
                     /locus_tag="ACHOWS_004869"
     CDS             complement(4295..5584)
                     /gene="sdaC"
                     /locus_tag="ACHOWS_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFQ0050328.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1526 a         1484 c         1602 g         1410 t
ORIGIN      
        1 aaatataagc ttgtgaattt ctttctggtt aatcaggaag aagatggtgg gtaattaaac
       61 ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc agagagaggt
      121 agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg gaaatgaccc
      181 gcctgggact gaaccagggg acagcgggta acgtcagtgt acgttatcag gatgggatgc
      241 tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt gtctttattg
      301 atggcaacgg caaacatgag gaaggaaaac tcccctcaag cgagtggcgt ttccatatgg
      361 cggcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc gttcattgta
      421 cggcagtttc cattcttaac cgaccgatcc ccgcgattca ctacatgatt gcggcggctg
      481 gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa ctttctgaac
      541 atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat gggcttatcg
      601 cttgtgaggc gaatctggag aaagcgttat ggctggcgca tgaagttgaa gtgctggcac
      661 aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc gatgaagaga
      721 ttgccgtagt gctggagaaa ttcaaaacct acgggttacg gattgaagag taatttcgta
      781 aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg gcatggtttg
      841 gtcggggggc tgttgggtct ttaaccgatg aggtgaaacg ccgtggttat cagaaggcgc
      901 tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcaaaagtg accgataaga
      961 tggatgctgc agggctggca tgggcgattt acgacggcgt agtgccgaac ccaacaatta
     1021 ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac ctgatcgcta
     1081 ttggtggcgg ttctccacag gatacttgta aagcgattgg cattatcagc aacaacccgg
     1141 agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc agtgtaccga
     1201 ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac tacgtgatca
     1261 ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc ccgcaggtgg
     1321 cgtttattga cgctgacatg atggatggta tgcctccagc gctgaaagct gcgacgggtg
     1381 tcgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg gcgctaaccg
     1441 atgcgctgca cattaaagcg attgaaatca ttgctggggc gctgcgagga tcggttgctg
     1501 gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt atgggattct
     1561 cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg ttttacaaca
     1621 cgccacacgg tgttgcgaac gccatcctgt taccgcatgt tatgcgttat aacgctgact
     1681 ttaccggtga gaagtaccgc gatatcgcac gcgttatggg cgtgaaagtg gaaggtatga
     1741 gcctggaaga ggcgcgtaat gccgctgttg aagcggtgtt tgctctcaac cgtgatgtcg
     1801 gtattccgcc acatttacgt gatgttggtg tacgtaagga agatattccg acactggcgc
     1861 aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg cttgaggata
     1921 ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc ggatgcgacg
     1981 tttgcgccgt atccggcacg ccgttacctt accaaccgca attgctgtaa attgccgtcg
     2041 atatgcaaat cggtttgtaa gcgggcaata tcgcggcaca gaaacgccat ctctttatgg
     2101 gtttctaatt ttttgcgcca cttttcggca accgcattca gattctcata tatcccttcc
     2161 agactcttaa actcgaccag cagctgcgtg gcgctttttg gtccgatccc cacaacaccc
     2221 ggtacctttg aactgctgat ccccgcaagt ccccagtaat cgggcaactg ctgcggttga
     2281 acgccaaatt ctttatcgat aaatggcgca tccagccaac gtttctggaa gtaatcacga
     2341 atacgtaatg tcggtgaaag taactgacag tagcctttat ctgtcgaaac aatcgttgcc
     2401 tgatgcccgg cctgtgtcac tttgaccgcc agcgtggcgg ctaagtcatc ggcttcgttg
     2461 ccgctggcgg accagcacgg gacgccgcgt tgctcaaagg cggcgcgtaa tgcaggcatc
     2521 tcgtcgtgca actcttccgg cattggcggt cgacccgctt tgtaatctgg taaacgctga
     2581 tgacgccagc cgctactgcg gttttcatca tcaaaaacgg cgaccgcgtg ggttggctgg
     2641 ctgtgcataa tgagctgatc gagcgcgtgc tggcaggttt cgacacaggg cgaaccctga
     2701 acggcatgaa tgcgacgaat aagattcagt gcatcgacaa taagcaaatg aacagccacg
     2761 ataaccccct taaagatcag gcgataaggg taacattcga tgccggaaca ggctatggct
     2821 ggttgagaaa acgggaagag gcctcgcaaa acgaggcctc cgggagagat taatcgcagg
     2881 caacgatctt cattgccagg ccaccgcgag aggtttcgcg gtacttggcg ttcatatctt
     2941 tacctgtttc gtacatggtc tcgataactt tatcgaggca gacgcgcggc tcgctggtac
     3001 ggcgcagcgc catacgcgcg gcgttcaccg cttttactgc cgcaatggcg ttacgctcga
     3061 tgcacggtac ctgcacctgt ccagcaaccg ggtcacacgt cagaccgagg ttatgctcca
     3121 tggcgatttc cgccgcgatg cacacctgcg ccgggcttgc gcctaacagt tctgccagac
     3181 cagctgccgc catagagcac gccacgccaa cttcaccctg gcaacccact tcggcaccag
     3241 aaatcgacgc gttcatctta taaagagaac caatggcgct ggctaccagc agataacgag
     3301 ccagtgagtt agcgttcact tcgcggataa acttatcgta gtacgccagc acagccggga
     3361 taatcccgca cgcaccgtta gtcggcgcag tcaccacgcg accgccagca gcattctctt
     3421 cgttcactgc cagtgcaaac atgttgatcc agtcaacaac cgccatcggg tcagtggtgg
     3481 ttttatcctg gctgaccagc atccggcgta gtgccgcagc acggcgtgga acgcgcagtt
     3541 tgccaggtaa cacgccttcg gtagaaatac cacgctcaat accgccacgc atgacttccc
     3601 agacgttcgc caggtgctgt tccagctctt ctttgctgtg cagcgccagc tcgtttttca
     3661 tcatcaggcc agagagtgac agcccggttt cctgacaatg tttttgcaga tcggctgctg
     3721 aactgtacgg ataaggaact tcaaccggtg cgctatcctg ctggccaaaa tgttcttcat
     3781 caacgataaa gccaccgccg atagagtagt aagtctggct gtaaacgact ttatcgcccg
     3841 ccagtgcggt aatgcgcata ccgttttcat gcagagaaag gttgtcggca tggaagttca
     3901 tgcactgatc aaccgggaac tccacttcat gctgaccgtt tgccagcatc aggcgaccat
     3961 gagtattcac atcctgaata aaaccaggga tggaatcgat atccacggta tccggcaggt
     4021 tacccgccag gcccataata atggcgatat cagtgtggtg gcctttaccg gtcagagaga
     4081 gcgagccgta cacgtcaacc accacgcggg tcacgtcttt aagcaggtta cgggcaatca
     4141 gatcgtcggt aaattgttta cccgctttca ttggtccaac ggtatgagaa ctggaagggc
     4201 caatgccgat tttgaaaata tcgaatacgc taatcatagg aaatacatcg cgttaaaacg
     4261 gaggaagcgc cgcccgaaag cggcgcgaaa ggacttagct gaacagagag tagaagattg
     4321 cggagattgc aatcagaccc atcacgacaa cgaatacgtt gctgatgtga ccgctgtact
     4381 tacgcattgc cggtactttc tgaattgcgt acatcggcat caggaacagg atcatcgcga
     4441 tgattgggcc gcccagggtt tcaatcatac ccaggatgct cgggttcagg gtggcaacaa
     4501 tccaggtcgt taccagcatg aacagcgcag tgatacggtt cagcttgttg atttcgatag
     4561 acttaccttt accacgcaga gatttaatca ccataccgtt gaagccttca cgtgcgccca
     4621 ggtagtggcc gaggaaggat ttggtgatag cgataatcgc gataatcgga gccatccacg
     4681 cgatgatcgg cgcgttaaag tggttagcca ggtaagacag aatcgagatg ttctgctctt
     4741 tagccgcagc caggtctgct ggagtcaggc tcagtacaca gctgaagacg aagaacatta
     4801 cggtcagcac catcatgatg tgtgcgaatg ccaggatctt cgagcatttc tgttctgcca
     4861 tatcgccgta ctcttcacgc ttcgcaacgg cgaaagaaga gatgatcgga gagtggttga
     4921 acgagaacac cattaccgga attgccagcc acagggtcat ccaaagaccg tttccggttg
     4981 cagatgcagt gtccagagac agcgtttcca gtgctgcgcc gttccactgc gggatcaggt
     5041 acagagccag cagcatcagt acgccaacaa acgggaatac cagaatactc atcgctttaa
     5101 cgatcatctg ctcaccgaag cgaacgatgg tcatcatacc cacgatcagg atcagcgaca
     5161 gaatcgcacg cggcggtggt gtcataccca gctggtgaga catgaagctt tcaactgtat
     5221 tggtgattgc cacgctataa accagcagaa tcgggtagat agcgaagaag tagagcaggg
     5281 taatcagttt acctgcgcca ataccaaagt gttcttctac aacctcggtg atgtcttcgc
     5341 ccgggttttt accagacagt acgaagcgag tcagaccgcg gtgagcaaaa aatgtcatcg
     5401 ggaacgcaag gatagccatg atgatcagcg ggatcatacc gccaacaccg gcgttgattg
     5461 gcaggaacag cacgcccgcg ccgattgccg tgccgtaaag gcccagcatc cacatggtgt
     5521 ctgtcttgcg ccaggcacta cgagagtctt tcgacgcaat cgtgctggtt tgagtcgttt
     5581 ccatctattt ctcctggagg aatgcaaata attcagcttt tagccagttc agcccgggaa
     5641 ggtgcctgac cgtaaaaatg aaatctgtct ataggcgatg gcttaatcat ttaccgccgt
     5701 aataatttga gggcggaaag atacacacaa gttatgtatc tatcagtgat cttgatccca
     5761 aatacaataa tctacttgga agatagatta tcccaacgct ttactctgta aatcaaaagt
     5821 aaaagagaat ttaagggcaa tatttgccca ttaaacgaca tctgacttct tccggggcgc
     5881 gcaatatatg taggttgaac tgaaaaaatc aatactttta gttaatcatg gcgataattt
     5941 ctttttcata gcgataaaat gtggtttatg aaaataggga aacgtttgcg ttacaacata
     6001 aattgtatga catattaact gt
//
LOCUS       DAZKJR010000221         5997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKJR010000221 DAZKJR010000000
VERSION     DAZKJR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.222"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            225..2072
                     /gene="ldtD"
                     /locus_tag="ACHOWS_004870"
     CDS             225..2072
                     /gene="ldtD"
                     /locus_tag="ACHOWS_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFQ0050329.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVVGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2253..2801
                     /locus_tag="ACHOWS_004871"
     CDS             2253..2801
                     /locus_tag="ACHOWS_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFQ0050330.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2828..3475
                     /gene="gloC"
                     /locus_tag="ACHOWS_004872"
     CDS             2828..3475
                     /gene="gloC"
                     /locus_tag="ACHOWS_004872"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFQ0050331.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3526..4716)
                     /gene="aspC"
                     /locus_tag="ACHOWS_004873"
     CDS             complement(3526..4716)
                     /gene="aspC"
                     /locus_tag="ACHOWS_004873"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFQ0050332.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETIDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4901..5989)
                     /gene="ompF"
                     /locus_tag="ACHOWS_004874"
     CDS             complement(4901..5989)
                     /gene="ompF"
                     /locus_tag="ACHOWS_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFQ0050333.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1498 a         1484 c         1540 g         1475 t
ORIGIN      
        1 gaaaactccg tttctgcact aaaaaagtgg cacattacgg cgcgcttttc tttaaacttc
       61 tttacattag gttatgtaaa aacgtatcgg cgtttatata ctgaagataa gcctgatgag
      121 taacaggctt gctcgtcata ctttcgtgag tattggcgtt gtacaggcaa gtcgtaaaat
      181 aacagcctgg ctattcagag tatgataaaa acagggggca agggatgttg cttaatatga
      241 tgtgtggtcg tcggctgtcg gcaatcagtt tgtgcctggc cgtaacattc gctccactgt
      301 tcaatgcgca ggccgatgag cctgaagtaa tccctggcga cagcccggtg gctgtcagtg
      361 aacagggcga ggcactgccg caggcgcaag ccacggcaat aatggcgggg atccagccat
      421 tgcctgaagg tgcggctgaa aaagcccgca cgcaaatcga atctcaatta cccgcaggtt
      481 ataagccggt ttatcttaac cagcttcaat tgttgtatgc cgcacgcgat atgcaaccca
      541 tgtgggaaaa ccgtgatgct gttaaagcct tccagcaaca gctggcagag gtggcgattg
      601 ccggtttcca gccgcagttt aataaatggg tagagttact gaccgatcct ggtgttaacg
      661 ggatggcacg cgacgtggtg ctctctgatg cgatgatggg ctatctccat ttcattgcaa
      721 atattccggt caaaggcact cgctggctat atagcagtaa accttatgcg ctttcaacgc
      781 cgccgctttc ggtgattaac caatggcagc tggcgctgga taaaggtcaa ttgcctacgt
      841 ttgttgtagg attggcaccg cagcatccgc aatatgcggc gatgcatgaa tcgttactgg
      901 ccttactcag tgacaccaaa ccgtggcccc aactgaccgg caaagcaacg ttgcgcccag
      961 gacagtggag taacgacgta ccggcgttgc gcgaaatatt gcaacgtaca ggcatgttgg
     1021 acgggggacc gaaaattacg ctacctggcg atgacacgcc aactgacgcg gtagtcagcc
     1081 catccgctgt tactattgaa acagcagaaa ctaagccgat ggacaagcaa actacgtctc
     1141 gtagtaaacc tgcgcctgtc gttcgtgccg cctacgataa tgaactggtg gaagccgtta
     1201 aacgttttca ggcatggcaa ggattggggg cagatggtgc tattggcccg gcaacgcgtg
     1261 actggttaaa cgtaacgccc gcccagcgtg ctggcgtgtt ggcactcaac atccagcgat
     1321 tgcgcttgct gccaacagag ctttctaccg ggatcatggt taacattcca gcctattcgc
     1381 tggtctacta tcagaacggc aatcaggtgc tggattcgcg agtcattgtc ggtcgccccg
     1441 atcgcaaaac gccgatgatg agcagtgcac ttaacaacgt agtggtaaac ccgccgtgga
     1501 acgtaccacc aactctggca cgcaaagata ttctgccgaa agtgcgcaac gatccgggat
     1561 atctcgaaag ccatggctat acggtgatgc gcggctggaa cagcagagaa gcgattgacc
     1621 catggcaggt tgactggtct acaatcacgg cctcgaattt accgttccgc ttccagcagg
     1681 ctccaggccc acggaactcg ctggggcgct ataaattcaa tatgccgagt tcagaggcca
     1741 tttatttgca tgacacgccg aaccacaatc tgttcaagcg tgatacacgc gcattgagct
     1801 caggctgtgt acgagtgaat aaagcttccg atctggcgaa tatgctgttg caggatgcag
     1861 gctggaatga caaacgtatt tctgatgcgc tgaagcaggg cgatacgcgt tacgtcaata
     1921 ttcggcagtc gattccggtg aatctctact atctgacggc ctttgttggt gcagatggtc
     1981 gtacccagta tcgtacagat atttacaatt atgatctgcc tgcgcgatcc agctcgcaaa
     2041 ttgtatcgaa agcggaacaa ttaatcaggt aaatgaagca gttcggggaa atcagttgtc
     2101 gtaataatta gcatgattgg gggcaattct ctgcagcccc cgtcactgct ggggttgagt
     2161 catcttgacg tctgctttac gggcggttaa ggtgcctctt gtgcgccaga agtgcatata
     2221 aacgataaca ttgacctgta gacttgatta tcatggacaa attcgacgct aatcgccgca
     2281 aattgctggc gcttggtggc gttgcactcg gtgccgccat cctgccgacc cctgcgtttg
     2341 caacactctc taccccacgc ccgcgcattt tgacactcaa taatcttcat accggagagt
     2401 caatcaaagc ggagtttttc gatggcagag gctatattca ggaagaattg gcaaaactta
     2461 accatttttt ccgcgattac cgcgcgaaca aaataaagtc catcgaccca ggattattcg
     2521 accagttgta tcgcctgcaa gggttgttag gtacccgcaa accggtgcaa ctcatttccg
     2581 gttatcgttc cattgatacc aacaatgaac tacgcgcccg cagccgtgga gtagcgaaga
     2641 aaagctatca cacgaaaggg caggcgatgg atttccatat tgaaggtatc gcgttaagca
     2701 atattcgcaa agccgcgtta tctatgcgcg caggtggtgt aggatattac ccacgtagta
     2761 actttgtgca tattgatacc gggccagcac ggcactggta gcaattgctt aacgaaacag
     2821 gggcagtatg aactatcgta ttattccggt caccgcattc tcccagaact gttcattgat
     2881 ctggtgtgaa caaacccgcc tggccgcact ggtcgatcct ggcggcgatg cggaaaaaat
     2941 caaacaggaa gttgacgcca gcggcctgac actgatgcag atcctgctga cgcatggtca
     3001 tctggaccac gttggtgcag cggcggaact ggcgcaacat tacggcgtgc cggtttttgg
     3061 cccggaaaaa gaagatgagt tctggctgca aggcttgcct gcgcaaagtc gtatgtttgg
     3121 tctggaagag tgccagccgc tgacgccgga tcgttggctg aacgaaggcg ataccatcag
     3181 catagggaat gtggctttac aggtgttaca ttgccctggg catacgccgg gtcatgtcgt
     3241 gttttttgat gatcgggcaa agctgctgat ttctggcgac gttattttca aaggcggagt
     3301 agggcgcagt gacttcccgc gtggcgatca taatcaactg atttcttcaa tcaaagataa
     3361 gttgctgcca ctgggggatg acgtgacatt tattccgggt cacggaccat tatccacact
     3421 tggttatgag cgcctgcata atcccttcct gcaagacgaa atgcccgtct ggtaaaggcg
     3481 cataaaaaag cccgcttttt aagcgggctt cattgttttt aacgcttaca gcactgccac
     3541 aatcgcttcg cacagcggag ccatattatc tggtgtcatc ccggccacgt ttacgcgacc
     3601 agaagcaaca gcatatacgc caaactcttc gcgcagacgc agcacttgtt cttttgtcag
     3661 gccactgaag gagaacatgc cgttctgttt gatgataaag ctgaagtcgc gatttgcgcc
     3721 tttttcctgc agcgtattga cgaacaactg acgcatacgc tgaatacgct ggcgcatatc
     3781 agtcagttct tgttcccaaa tcgcacgtaa cgcatcgttg ctcaggatgg tggcaacaac
     3841 agaagcgccg tgtgctggtg ggttagagta gttagcgcga atcgccgctt tcatttggct
     3901 gaatgcgcga tcaatggttt cactgtcagc agcaaccaga gtacaagcgc caacacgctc
     3961 gttgtacagg ccaaagtttt tagagtagga actggcaaca atcagctctt tatgcatagc
     4021 cgcgaaagcg cgcagtcctt cagcatcttc ttccagacca cgggcaaacc cctggtaagc
     4081 gaagtcaaac agcggtaacc agcctttctc aacggaaagt tgtgccagtg tctgccattg
     4141 ttccagcgta gggtcgatac cggttgggtt atggcagcag ccatggaaca gcaccacgtc
     4201 gccagcctga gcttcgttca ggctgttaat cagtgcatcg aagtcaaggg tgtgattttc
     4261 cgcatcataa taagcgtatt cacgaacttc cagacctgca gagttaaaga cgctcttatg
     4321 gttcggccag cttgggttgc tcacccacac acgcttaacg ctggtatttt ttgccaggaa
     4381 atcggcagcc acgcgtagtg cgccagtgcc acccggagtc tgtgccgtgc gagcacgttt
     4441 gtcattgatc agggcgctac ctttaccaaa cagcagttcc tgagtgcagc gaccaaattc
     4501 agggatgccg tcaatgccga ggtaattttt ggtggtttca ttttcgagca gatactgttc
     4561 agcctttttc acactggtca gtaccggggt tttgcccgtc tcatctttat agacaccaat
     4621 cccgaggtta attttgccgg gacgttcatc ggcacgaaac agatcggcca ggcccagaat
     4681 cgggtcggca ggagcggcgg taatgttctc aaacatgacg aggttccatt atggttacag
     4741 aagggaagtc cgctatcagg gtaacgggag atttacaaaa ttccaactat tactgatgaa
     4801 aacgcaggct gtttttgcaa gacgtgagat tgctctggaa ggcataaaaa aaacaggacc
     4861 aaagtcctgt tttttcggca tttaacaaag aggtgtgcta ttagaactgg taaacgatac
     4921 ccacagcaac ggtgtcgtca gaacctacgc ccagtttgtt gtcagaatcg atctggttga
     4981 tgatgtagtc aacataggtg gacatgtttt tgttgaagta gtaggttgcg cccacttcaa
     5041 agtagttaac cagatcaaca tcaccgatac cttctacgtc tttcgcttta gatttggtgt
     5101 aagcgatgga cggacgcaga ccgaaatcga actggtattg cgcaactaac agaacgtctt
     5161 gcgttttgtt ggcgaagccg ctgatgtttg taaatttatt agtgatcggc gtagcgttac
     5221 gggtttcacc gtagttcgct gccaggtaga tgttgttcgc gtcatacttc agaccagtag
     5281 cccactgttc agctttttta ccgttgccaa gaggttgagc ttcttgcagg ttggtacggt
     5341 cagcggcgcc ataagcacca acgataccaa agccttcgta ttcgtagctg atagaaccgc
     5401 caacaccgtc gccgttagag cggcgtgcag tgtcacgctc gtttttaccc aggtactgaa
     5461 cagcgaagtt caggccatca accagaccaa agaagttgga gttacgatag gtagcaacgc
     5521 cgccaacacg accaacgaag aagtcatcgc tgtatgccgt gtcaccgcca aactcaggca
     5581 gcatatcggt gtaacccagt gcatcataaa ccacaccgta gttacggccg taatcgaaag
     5641 aaccaacgtc agcgtattta agacccgcga atgccagacg cgttttgtta ccagtttgag
     5701 cgtcagcgcc ttcagagttg ttaccctgga agttatattc ccactgacca taaccggtca
     5761 gatcggaatt gatttgagtt tcccctttaa aaccaagacg ggcataggtc atgtcaccat
     5821 tgccaccgta actgttttca ccgttaccct tggagaaata atgcagaccg acagctttac
     5881 cgtacagatc tactttgttg ccatctttgt tatagatttc tgcagcgttt gcagtacctg
     5941 ctactaacag agcagggacg atcactgcca gaatattgcg cttcatcatt atttatt
//
LOCUS       DAZKJR010000222         5974 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKJR010000222 DAZKJR010000000
VERSION     DAZKJR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.223"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            229..810
                     /gene="mdaB"
                     /locus_tag="ACHOWS_004875"
     CDS             229..810
                     /gene="mdaB"
                     /locus_tag="ACHOWS_004875"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFQ0050334.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     ESDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            841..1155
                     /gene="ygiN"
                     /locus_tag="ACHOWS_004876"
     CDS             841..1155
                     /gene="ygiN"
                     /locus_tag="ACHOWS_004876"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFQ0050335.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1200..2087)
                     /locus_tag="ACHOWS_004877"
     CDS             complement(1200..2087)
                     /locus_tag="ACHOWS_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0050336.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTMLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2084..3031)
                     /locus_tag="ACHOWS_004878"
     CDS             complement(2084..3031)
                     /locus_tag="ACHOWS_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0050337.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAGLTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3042..4079)
                     /locus_tag="ACHOWS_004879"
     CDS             complement(3042..4079)
                     /locus_tag="ACHOWS_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0050338.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4088..5071)
                     /locus_tag="ACHOWS_004880"
     CDS             complement(4088..5071)
                     /locus_tag="ACHOWS_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0050339.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILIFDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5068..5877)
                     /locus_tag="ACHOWS_004881"
     CDS             complement(5068..5877)
                     /locus_tag="ACHOWS_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0050340.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1695 a         1625 c         1368 g         1286 t
ORIGIN      
        1 tttccgaaaa actactttat catttacata atacatctct cctgtgggaa cttataggaa
       61 gttgatttca gtattgtcat tttcagaaaa tatttatccc gcatcggtga atcagagcaa
      121 gatcagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt caccgtaccg
      181 ctctgtgacg tactatagtc gacaaacgtc tcaccttgag gttaaaaaat gagcaacatc
      241 ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa cgacaccctg
      301 accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgtccgcat cgttcgcgcc
      361 gagagcgact acgatgtcaa agccgaagta caaaactttc tctgggctga tgtggtgatc
      421 tggcagatgc ctggctggtg gatgggcgcg ccgtggacag tgaaaaaata cattgatgat
      481 gtattcaccg aaggtcacgg gactctgtat gccagcgatg gtcgtacccg caaagatccg
      541 tcgaaaaaat acggttctgg cggtctggta caaggtaaaa aatatatgct ttccctgacc
      601 tggaacgcac caatggaagc tttcaccgaa aaagatcagt tcttccacgg ggttggcgtt
      661 gacggtgtgt atctgccgtt ccataaagca aaccaattcc ttggtatgga accgctgccg
      721 acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac tgaagaatat
      781 cgcaagcatc ttgtggaaat ttttggttaa ctagagctct ggctttagaa ggagttaacc
      841 atgattacag taatcgcaga aatccgtact cgtcctggtc aacatcaccg tcaggcagtg
      901 ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg ccacggctat
      961 gcgccaatgg tggattgcgc tgctggcgtg agtttccagt ctatggcacc ggattctatc
     1021 gtgatgattg agcagtggga aagcatcgcg catcttgaag cgcatctgca aaccccgcac
     1081 atgaaagcat atagcgaagc cgtaaaaggt gacgtgctgg agatgaatat tcgtatcctg
     1141 cagccggggc tttgacattt ctaaaaagag gccggtgctg ctaccggtct cagcccagat
     1201 tagccattct ttcttcccgt tttcagtcct cgatggaaat cgttaattcg cttaatggtt
     1261 ttaaccgcac gctgcggcac tgcggaagct accgaccaga caatcatttc cagacacaac
     1321 aataccgtac catgtagcgg cgttttccct ttttcatccc cgcgcggaac atgaatcacg
     1381 atactggcgt ctttgctaaa acgtgaatcc agcgcgtttg tcagcaaaat aacagggatc
     1441 cccagccgcc tcgcctcacg aagcattgtc agcccttccc ggtgcgcaga tttttgcgcc
     1501 atcatgatca gaacatcgcc acgttgaagc gcgatcagtt gctcggcaag gccaataccc
     1561 gtacggttta gtgccgtggc aggcaaacct atgcggttga atagtcgtgc ggtatagtcc
     1621 gccagaatac ccgaagcgcc aatgccaaaa atagcgactt gccgtgcctg caccaacaac
     1681 gctacagctt gcgcaagcgc gtggcggttt tcaggctctg acaggacatt acaggtatac
     1741 aaatgccctt caagaacaaa gtcgattgcg gtgttaacat cgctcgtcaa gttactcacc
     1801 gtcgtagaca tcttttcgct ggaactaagg gctggaccaa gccactgttc cagagtgcgt
     1861 ttcagatcac gcagcccggc aaaccccagg gcctgtatag ccctgataac tgtggcatcg
     1921 gaagtgttca acgtggccgc aatttccatc gccgtttgct cgagtaccac ttcacgatgc
     1981 tcattaatgt agctggcgac aactcttaac ccaggcgtta actgactcgc gcgagtgcga
     2041 aaacgctcac caaaaatatc aacccgcact ttatctttct ggatcattta gcctccggta
     2101 gaggtcgccc tgcaatttgt gattgtatct gcgcagccat cagccccaga gatgcaaagg
     2161 agttagaaat tgcctcttca cgagaaatca gcacataacg cccggaacga caggcagtca
     2221 gaaactgaca ccatcccggc atgacttttt ccattgctgc cagctcatcc tgaggtttac
     2281 caccggtgtc accgcgccag gttgcaaaaa caaaatcggc atccagttca ggtaaacgct
     2341 ccgcgctcac atccattcgc ccaccttcag gaatgctctc aatcagcggc ggaaaacgaa
     2401 aacccgcatc gcgtaatacc cgccccagcg aatgatagct atgcatcaca ttaatttttc
     2461 cctggtttgc ctgaatcacc gataccgtta ttttttggct gtccagcgtc gccttcagcg
     2521 cattgatttg tgcctgatag cggcgttcaa gtatcgccag ctgcgactgc gttcctgtaa
     2581 gtcctgccag cttgcgatag atttctggcg cacccccttt gagatgatca atgctcaccg
     2641 tgggcgcaat tttttcaagt tgttcaatgg gcgtattacg ggtcggttct gtgatgatca
     2701 ggtcaggctt tgccgccaca atcgcttcaa tatcaatatc agcggtacca ataaaggcga
     2761 tcgatgaatt atcaaagtcg acacctgtca ataacgcgcc agatcgaatg aaatggctac
     2821 cgtcaggccg agttcgcccg tggctggcga caggaggtac accgagctca atcagaggaa
     2881 tagtgatatc aagatcgtgt aaagagacaa ttctctttgg atgagcagga acagtgactt
     2941 tgcgccctaa atcatcagta aatacctgaa caggttctgt tgcacgggaa aaaaatgaca
     3001 acagtatcag cagagaaaaa aataaacgca tataactccc gttaaaaact gttcctgcgc
     3061 tgccagagca gtagcaaaaa aaatggtcca ccaatcagtg agataatgat tccggcaggc
     3121 agttgcaaag gcaaaaaagc caaacgacca atgctatccg ccagaataac taacaacgcc
     3181 cccgataccg cgctccccag cagtaatgtc gtctgcccgc cacgtagaat aaaacgtgat
     3241 atatggggtg ctatcaatcc gacaaaaccg atgttaccaa cacacgaaac gcagacggct
     3301 gtcaggatta ttggtgcggc gacacgtaac aacgccagac gagaactatt cacccctaac
     3361 ccggttgcaa cctgatgacc aagcagagcg atatccgccg tcctggcggt gaacagcaat
     3421 aaaagagcgg ctggcaacat ccaacaggct gaaataccga ctaacatcca gttagctgca
     3481 tgcaggcttc ccgataacca catcaatgca gtttgtacat ctcgaacatc tgcagtggtc
     3541 ataaatacac cgataccggc ggcgaaaaac caggaaacac caataccaat cagaacaaat
     3601 cgcgggcggg aaatatcgcg cgcacaaaaa atcacgatta aagccaccaa caagccacca
     3661 gcaagaccag ccactggacg ccagaacatt cccagcattg gaaactggaa aatcagccac
     3721 agcaccgcca cgctacatcc ttctttgacg ccgattaaac caggatcggc aaggccgtta
     3781 cgcgcaatgg attgcatcgc ggcccccgcc atacctaaca tggctccgca tagcaatgcc
     3841 atgatgactc tcggcaagcg aatatcctgc acaatataac gtgcgtctgt gcttacgttt
     3901 tctggagcaa acaacgctcg tcctacttcc gatgccggga cagaaaaaga accatgcatc
     3961 agaccgaata tcagtattcc aaccgcaaac agaaacagcc caccaagcaa caccagattt
     4021 ttagggcgta ccagtgtcga aaacttacca acacgtagtg ggcgtagccc gacgttcatt
     4081 atccgactca tttgaacatc ctcgtcgcca taatgacaaa aacaggcgca ccaactaacg
     4141 ccgtcatgac accggtcgct aactcatggg gcgtgaaaag cgtccgagca ataatatctg
     4201 ccagtaataa caaaagcgcg ccgacacaag ctgaaagagg gagcagaacg cgcagatccg
     4261 ctgataccag acgccggaca atctgcggta ctagcaggcc gacaaaacca atggggccag
     4321 caatcgatac ggctgcgcca cagagcaacg cgatcgcaag cagggtgaat gttcgcgtgc
     4381 ggagtaccga gacgcccagc ccctgcgcca tgcgatcgcc cagtgccaac atgttaagag
     4441 agggcgcaag gtaaatggct aacacaaatc cccccagaga aaaccaggcg gacgtaccca
     4501 gcgtcaccca atcctgcccc gctaaatcac ctgctagcca tgtccgcatc gccagcagtg
     4561 tttgttcgtc aaaaatcagg attgcagccg ttattgagga cacgaacgcc gatagcgcca
     4621 cgccgcataa agtaactttc attggcgtta accctgaacg ccctgcggag gaaagcagca
     4681 atatcaacag gaacaataac gcgccccccg tagatgccag cagtgggcgt ccaaccggaa
     4741 aggccagtcc aagcgcactg gcggcaacga cagcaagagc ggctccggca tttaagccta
     4801 aaatatgtgg ctcccccaat gggttacgaa tgacagcctg caacaaagca ccagcaacac
     4861 caagagaagc accggtaagc aatgctgccg ctaaacgcgg caaacggagt cttacaatta
     4921 cgttgtgatc gaagttttgc ggatcaaaat gaagaaatgc ctgcaacacc gtttgtggtg
     4981 caataaatcg ggcaccaatc cctggatata ccacgcatcc caccagcagt aaggcgagga
     5041 aaattacagt aaccagccct gtgcgcatca tactttttct acaccacgaa acggcatgaa
     5101 gaacggcttt cccgtcagag gatttattga tgcgtgaaca tcaacgtcaa agacagcttt
     5161 taccagttca ggcgtgcaat gctccccctc attcaacaca cgcaccactt tcccctggcg
     5221 taaaaatatc aacgtatcgc cataattgac ggcaaaattc agatcatgca acaccacgac
     5281 gactgtacgc ccgtgatgac gcgttaaatc atgtaataac tcaagaattt caacctgata
     5341 acgtaaatca agccaggttg tgggttcatc cagcaggata taaggcgtct tttgcgccaa
     5401 caccatcgca atccagcaac gctggcgctg cccaccagag agtttttcaa caggtaaatg
     5461 tgcaaactcc tgcgtaccgg ttaatttcag agcctcttct accgcctgct cgtcggcgtc
     5521 gctccattga cgaatgaaat tctgccaggg aaaacgccca cgcgacacca gttcataaac
     5581 ggttaagccc tccggcaata atggcgactg cggcaagatc ccaagtctac gggataacgc
     5641 tttcgttggt tgctcgtgaa tcgctttgcc atcaagcaat atcgaaccgc ccataggttg
     5701 caagatacgt gcaatggtgc tgagcaacgt cgactttcca cagccgtttg ccccaaccag
     5761 aacggtcatt ttttctgtgg gaacggagaa agaaacacca tcaacaatga tcttcttgtg
     5821 atacccggca gaaacattgt cgaggatcag cccctgccct tttgcgttat gtgccacgtg
     5881 tacgccaact taatcaaaga caaacaaaaa taaaactcat tctcctttga gagtttgacg
     5941 ccacaaaatg tagtagtcaa gcagttaaaa gcca
//
LOCUS       DAZKJR010000223         5954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKJR010000223 DAZKJR010000000
VERSION     DAZKJR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.224"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            104..1438
                     /gene="adeQ"
                     /locus_tag="ACHOWS_004882"
                     /gene_synonym="yicO"
     CDS             104..1438
                     /gene="adeQ"
                     /locus_tag="ACHOWS_004882"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFQ0050341.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISSVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1491..1943
                     /gene="yicN"
                     /locus_tag="ACHOWS_004883"
     CDS             1491..1943
                     /gene="yicN"
                     /locus_tag="ACHOWS_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFQ0050342.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2154..3344
                     /gene="nepI"
                     /locus_tag="ACHOWS_004884"
     CDS             2154..3344
                     /gene="nepI"
                     /locus_tag="ACHOWS_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFQ0050343.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3385..3678)
                     /gene="yicS"
                     /locus_tag="ACHOWS_004885"
     CDS             complement(3385..3678)
                     /gene="yicS"
                     /locus_tag="ACHOWS_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFQ0050344.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3900..4718
                     /gene="nlpA"
                     /locus_tag="ACHOWS_004886"
     CDS             3900..4718
                     /gene="nlpA"
                     /locus_tag="ACHOWS_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFQ0050345.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4722..5645)
                     /gene="yicL"
                     /locus_tag="ACHOWS_004887"
     CDS             complement(4722..5645)
                     /gene="yicL"
                     /locus_tag="ACHOWS_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFQ0050346.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5812..>5954)
                     /locus_tag="ACHOWS_004888"
     CDS             complement(5812..>5954)
                     /locus_tag="ACHOWS_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pas-pac-pac sensing his kinase"
                     /protein_id="HFQ0050347.1"
                     /translation="STPERQFSISVLDDDNKENLPTDTAPCFTIGIPDEYTKAIELLN
                     IK"
BASE COUNT         1437 a         1327 c         1522 g         1668 t
ORIGIN      
        1 atatccatta atgtgcaatt gaaatgtgat aattatcact gaaccgttgt tgtcatttgt
       61 tttacaaaag caagggatta aatctcctcc aatggacaaa aaaatgaata atgacaatac
      121 cgattacgtg agtaatgaat cagggacgct ttcgcgatta tttaaactac ctcagcatgg
      181 gaccaccgtc cgcacagaat tgattgcggg gatgaccact tttttaacca tggtatacat
      241 cgtttttgtg aatccgcaaa tcctcggcgc ggcacaaatg gacccgaaag tggtgtttgt
      301 taccacctgt ttgattgccg gtatcggcag tattgcgatg gggatatttg ctaacttacc
      361 cgtggcgctg gctccggcaa tggggctgaa cgccttcttt gcgttcgtgg tcgtgggggc
      421 gatgggcatc tcctggcaga ccgggatggg cgcgatattc tggggcgcag ttgggctatt
      481 tctgctcacg ctgtttcgta tccggtactg gatgatctcc aacattccat taagtttacg
      541 tattggtatc accagcggaa tcggattatt tatcgcctta atgggattaa aaaatactgg
      601 cgttattgtc gccaataaag acaccctggt gatgattggc gatttaagtt ctcacggcgt
      661 gttgttaggt attttagggt tttttattat aaccgtgttg tcatcacgtc attttcatgc
      721 cgcggtgctt gtttctattg tggtgacgtc ttgctgtgga ttatttttcg gtgatgttca
      781 ttttagcggc gtctattcca ttccgcctga tattagcagc gtcattggtg aagtggattt
      841 gagcggcgcg ttatcacttg aactcgccgg tatcattttc tcctttatgc tgatcaacct
      901 atttgattca tcaggaacat taattggtgt aactgataaa gctggcttaa tagatagtaa
      961 cggtaaattc cctaatatga ataaggcgct gtatgttgat agcgtcagtt cggtggcggg
     1021 tgcgtttatc ggcacctcgt ctgttaccgc ctatattgaa agtacttctg gtgtggcagt
     1081 cggtggtcgc acggggctga ctgcggttgt ggtcggcgtt atgttcctgt tggttatgtt
     1141 cttctcaccg ctggtggcga tggttcctcc ttacgcaacc gccggagcgt taatctttgt
     1201 tggcgtgttg atgacctcaa gtctggcgcg cgttaactgg gatgatttta ccgaatcggt
     1261 acctgcgttt attaccacgg tgatgatgcc ctttactttc tcgatcaccg aagggattgc
     1321 actcggcttt atgtcgtact gcatcatgaa agtgtgcacc gggcgctggc gcgatctgaa
     1381 cctgtgtgtg gtggtggtcg cagctctgtt tgcactgaag attattctgg tggattagta
     1441 actggcgagg gtttccagat atcatgagtt ctgattacgc aggagaactc atgatctgga
     1501 taatgctcgc cacgctggcg gtagtgtttg tggttggttt tcgggtgctg acatccgggg
     1561 ccagaaaagc gattcgccgt ctcagcgatc ggctgaacat cgatgtcgta cccgtggagt
     1621 cgatggtcga tcaaatggga aagtcagccg gtgacgaatt tttacgttat ttgcatcgtc
     1681 cggatgagtc gcacctgcaa aacgccgcgc aagtgttgct catctggcaa attgtcattg
     1741 tcgatggtag cgaacagaac ttgctgcaat ggcatcggat tttacaaaaa gcccgccttg
     1801 ccgctcccat taccgatgct caggtcaggc tggcgctggg ttttctgcgc gaaaccgaac
     1861 ctgaaatgca ggatattaat gcttttcaga tgcgctataa cgcgttcttt cagcctgccg
     1921 agggcgttca ctggctgcat taaaaccgct tcgtcttatc agcctacaga ccgcgcctga
     1981 cctgtagggt ggataaggcg ttcacgccgc atccggtaat ctttgccctg ttccgtttcg
     2041 atcttaactc cagctcaagc aacaccgtaa aagttgcaaa aacgttaaat acgtcacaca
     2101 tttcaatgtt aaagtggtcg gctttccctc tgaaacatgc cacgggtaac accatgagtg
     2161 aatttattgc tgaaaaccgc ggcgcggatg ccatcacccg accgaactgg tcagccgttt
     2221 tctcggtggc gttttgcgtc gcctgtctga ttatcgttga gtttttgcct gtcagtttgt
     2281 tgacgccaat ggcccaggat ttaggcattt cggaaggggt tgccgggcaa tcggtgaccg
     2341 tgaccgcctt tgtggcgatg tttgccagtt tgtttattac ccagaccatt caggccactg
     2401 accgccgcta cgttgtcatt ctgttcgccg ttttgctgac gctttcctgt ttgctggttt
     2461 cctttgccaa ctcattcagt ctgctgttaa tcggtcgcgc ctgtctgggg ctggcgctgg
     2521 gcgggttctg ggcgatgtcg gcgtcgctga ccatgcgtct ggtaccgccg cgtacggtgc
     2581 cgaaggcgct gtcggtgatc ttcggcgcgg tttctattgc gctggtgatt gccgcgccgt
     2641 tgggcagttt tttaggcgag cttatcggtt ggcgcaatgt ctttaatgcg gcggcggcga
     2701 tgggtgtgct gtgtattttc tggattatca aatcactgcc ttcgctgcca ggtgaacctt
     2761 cgcatcagaa acaaaatact ttccgcttat tacaacgtcc gggagtgatg gccgggatga
     2821 tcgccatctt tatgtccttt gccggacagt ttgctttttt cacttatatt cgcccagtgt
     2881 atatgaatct ggcgggattc ggtgttgatg gcttaaccct ggtgctgctg agttttggta
     2941 tcgccagctt tgtcgggaca tcgctttcgt cgtttattct caaacgttcg gtgaaactgg
     3001 ccttagcggg cgcaccgttt gtgctcgccc tgagcgcgct ggtactgacg ctgtggggaa
     3061 gcgataaaat tgttgctact ggtgtggcga ttatctgggg gctgaccttt gctttaattc
     3121 ccgttggttg gtcaacgtgg atcactcgct cattggccga tcaggcagaa aaggctggat
     3181 ctattcaggt ggccgttatt cagcttgcca atacctgcgg tgcggcaatc ggcggttatg
     3241 cgttggataa cattggtctg acttcgccgc tgatgttgtc cggcacattg atgttgctga
     3301 ctgcattgtt ggtgactgca aaggtgaaaa tgaagaaatc ctgatatcag gtaaagaagc
     3361 gttggtcagt ggttgttgcc gttcttacat atccgggcat tctaccttac ccaacgcttc
     3421 ccaataattg ctctgattat tacgttccat cgccacgatg gcctcgcgta tatgttgttt
     3481 attgttttca tctgacaaca ttaacttttc ccacgcttca tctgataatg acgcctgtgg
     3541 cgtgcaaatt ttacgtaaat cttgcaatac cgtggttttc tcttttgccg actgtagaga
     3601 actgaatggc gactcggcct gagaaatggc tggaaaaatc aggaatacgg taatcatgag
     3661 tagcatcgtt ggcttcatag cggcaacctt ctgcaaatgt gcatcgaatg aagcataagt
     3721 gtagtttgct tttgccgctc tcatcatgct gtacgaaagg tgtttgcgtg gtcagtaaga
     3781 agtgccagac tttatattcc acttttattc ctttttattc ttatcgatag cgtttcgttt
     3841 tttaaactgc agcgacctta ccgctatagt caggtaatca ttaataaaag gataaaaaaa
     3901 tgaaactgac aacacatcat ctacgggcgg gggccgcatt attactggca ggaattctgc
     3961 tggcaggttg cgaccagagc agcagcgatg aaaagcacat taaagtgggc gttattaatg
     4021 gcgcagaaca agatgtcgcg gaagtcgcta aaaaggtggc aaaagagaaa tatggcctcg
     4081 atgttgaact ggtaggattt agcggttcgt tgctaccgaa cgatgcgaca aatcatggcg
     4141 agcttgatgc gaacgtcttc cagcatcgac cctttcttga acaagataat caggcgcatg
     4201 gttataaact ggtagcggtg ggaaatacgt ttgtcttccc catggcgggt tattccaaaa
     4261 aaatcaaaac cgtggcgcaa ataaaagaag gcgcgacagt ggcgatcccc aacgacccta
     4321 ccaaccttgg tcgcgcgctt ttactgttgc aaaaagagaa gctaattact ctgaaagagg
     4381 gaaaagggtt actacctacc gcgctggata taaccgataa tccacgccat ttgcagataa
     4441 tggagctgga aggggcacag ttgccacgtg tactggacga cccgaaagtg gatgtagcga
     4501 ttatcagcac cacttacatc cagcaaacag gactttctcc ggttaacgac agcgtattta
     4561 ttgaagataa aaattcgccg tatgtgaata ttctggtggc acgggaagat aataagaatg
     4621 cagaaaacgt gaaggaattt ctgcaatctt atcaatcacc cgaagtcgct aaagcggcag
     4681 aaaccatctt taacggaggc gcggtgcctg gctggtaatt ctcactcatg ccgcgccgga
     4741 cgatttattt ttctggcgcg gcggcgggaa tccattgaaa tcaaaatcac cgatgacaga
     4801 atcagcagcg ttcccagcca gtccggcaat gtgaaggtga tccccagcaa cagcaaagag
     4861 agcagcgcgc tacttaatgg ttctgcacag cttaaaatgc tggcttttgg accgccaatt
     4921 aattgtgctc ctttcaggta cagactaaat gtcagggacg taccaatgac caccaaataa
     4981 aaaaacgcca gaatcaaact gccgttaacg acaaagtttg tcccttgtct ggcataaaag
     5041 ggcagcagga tcagaccgcc aatcagcata ctccagccga cgaccggtaa cgtgccatag
     5101 cgggcaatca gcgttgaggg ataggtggta tagaatgcag cagcaaatgc cgaggcaatg
     5161 ccccagaata acgcggcagg agagatcgat aatgacgtcg gattaccgtg tgtcaccaat
     5221 aaaaaagtcc cgatcagcga tgtcaaaata gcgcagaaaa ccagaatgcc cgggcgggat
     5281 ttacgcacca gtgagaacca ggcgacgata atagtcggtg agaggaattg cagcaccgtt
     5341 gccgtggctg cgttcgattt ttctatggtt agcaaaaaag tgagctgtac ggttagcgcg
     5401 ccaaccacgg aaaaaatcag taggctaatg gcatctttgt gattgttaat aatagaaaag
     5461 attttatcac catgaacaaa tgatagcgtc agcagaatca aaccggcgaa tatcaaacgc
     5521 gtcatagtca aaaactgcga cgacatctgg ctttgctcca tgatgtattg cgcgcagacc
     5581 cctgaacttc cccacaacac ggcggcaatc agaacgttca gcatcccctt tctggtggaa
     5641 cccatttttc cctctgcata ctgttttgtc ttttcatcaa tatacgttaa ggataccact
     5701 accgatgacg gtgagggagt agagtaatca atcagtttta gtgaatggag gaaataatga
     5761 catataacaa ttattcatat ggtatttaaa aaaactcaat atgaaattta attatttaat
     5821 gttcaataac tctatggctt tagtatattc atcaggaata ccaattgtaa aacaaggtgc
     5881 ggtatcggta ggtagatttt ctttgttatc atcatccaga acagaaatag aaaactgtcg
     5941 ttcaggtgtt gatg
//
LOCUS       DAZKJR010000224         5869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKJR010000224 DAZKJR010000000
VERSION     DAZKJR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.225"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..186)
                     /locus_tag="ACHOWS_004889"
     CDS             complement(<1..186)
                     /locus_tag="ACHOWS_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFQ0050348.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVW"
     gene            complement(290..1027)
                     /gene="bamD"
                     /locus_tag="ACHOWS_004890"
     CDS             complement(290..1027)
                     /gene="bamD"
                     /locus_tag="ACHOWS_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFQ0050349.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            1162..2142
                     /gene="rluD"
                     /locus_tag="ACHOWS_004891"
     CDS             1162..2142
                     /gene="rluD"
                     /locus_tag="ACHOWS_004891"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFQ0050350.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            2139..2870
                     /gene="yfiH"
                     /locus_tag="ACHOWS_004892"
     CDS             2139..2870
                     /gene="yfiH"
                     /locus_tag="ACHOWS_004892"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFQ0050351.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            3000..5573
                     /gene="clpB"
                     /locus_tag="ACHOWS_004893"
     CDS             3000..5573
                     /gene="clpB"
                     /locus_tag="ACHOWS_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0050352.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1467 a         1407 c         1595 g         1400 t
ORIGIN      
        1 ccagaccatc atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc
       61 aaggcaacga gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg
      121 cagagaatcg taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg
      181 aaacaagtga gcgtttccgg attcttacac agccactgga tcggtcaact gatccttaac
      241 tgatcggcat tacagctcaa gggctgccgt tttgtgtttc aggtttctgt tatgtattgc
      301 tgctgtttgc ggcgatgatt ttcgctactt tttcagcttg cgcattcaac tgcatctgac
      361 ggtatgcatt ttccatcagc ggcagcgcat cacgcgtagc ctgggtatcc gggtagtcgc
      421 gcaacatgcc ttctacgcgg ttaacgacgg caacccatgc accacgttct gtatagtact
      481 cggcaacgga gtattcatat ttcgccagac gatctttcag gaataccaga cgtttggtgg
      541 catcggtggt gtactgactg ttcggatagc cgcgcaccag tttggaaaag tcactaaacg
      601 cagctcgtgc atgttgagga tcgcgatcgc tacggtcaac gccaaagaac ccttgcagcg
      661 cactgtcatc cagcgccata ttggtcaggc cacgcatgta catgacataa tcgatattcg
      721 gatgggtcgg gttaaggcga ataaaacgat cgatggcagc ctgtgctaac ggcaaatcgg
      781 cgtttttata gtaggcgtag atgagatcca gctgcacctg ctgcgaatac ggaccaaacg
      841 gatagcgatt atctaacgct tccagttgcg ttattgcctg tctccagtta ccgtcctgca
      901 gcttttgttg tgcagtcgcg taaatttcat ttggcggatt atcaggtact tcttcctttg
      961 accccgagca acccgccaaa aacaggctta gtgtggcggc tgccaccaga tatttcatgc
     1021 gcgtcatgac gttttgactt tcctcaaaat gtaatacggg agattctctg ttcctgctcc
     1081 cggttaagac cagctacaat agcacactat attaaacggc aaagccgtaa aaccccaacg
     1141 ataaacgaag aagcagtata tatggcacaa cgagtacagc tcactgcaac ggtgtccgaa
     1201 aaccaactcg gtcaacgctt agatcaggct ttggccgaaa tgttcccgga ttattcacgt
     1261 tcgcgaataa aagaatggat cctcgaccag cgcgtgctgg ttaacggcaa agtttgtgat
     1321 aagccgaaag aaaaagtatt gggtggcgag caggtggcca tcaacgctga gattgaagaa
     1381 gaagcgcgtt ttgaaccgca ggatatcccg ctggatatcg tctatgaaga tgaagatatc
     1441 atcgtcatta ataaaccacg cgacctggtg gtacatcctg gcgcgggtaa cccggatggt
     1501 acggtactga atgcgttgct tcattattat ccgcccattg ccgatgtacc gcgtgcgggc
     1561 atcgtccatc gtctggataa agacaccacc ggcctgatgg ttgtggcaaa aaccgttccg
     1621 gctcagacgc gtttagtcga atctttgcaa cggcgtgaaa ttactcgtga gtatgaagcg
     1681 gtggcaattg gtcatatgac cgctggcggt acagtagatg aaccaatcag ccgccacccg
     1741 accaaacgta ctcacatggc ggtgcatccg atgggtaaac cggcggtcac tcactatcgc
     1801 atcatggaac acttccgtgt gcacacgcgt ctgcggttgc gtctggaaac cggacgtacg
     1861 caccagatcc gcgtgcatat ggcccatatc actcatccgc tggtgggcga tccggtttat
     1921 ggtggccgtc cgcgtccgcc aaaaggtgct tcggaagcgt ttatctccac gctgcgtaag
     1981 tttgaccgcc aggcgcttca tgcaaccatg ctgcgtcttt atcacccgat ctccggtatc
     2041 gaaatggaat ggcatgcgcc tattccagaa gatatggtgg agctgattga ggtgatgcgc
     2101 gccgatttcg aagagcataa ggatgaagtg gactggttat gagtaagctg attgtcccgc
     2161 agtggccgct gccaaaaggt gttgcggcct gtagctccac tcgtatcggc ggcgtgagct
     2221 tgcccccgta tgattcgctc aacctcggtg ctcattgtgg cgataacccg gatcacgttg
     2281 aagagaatcg taagcgactt tttgctgcgg gcaatttgcc ttcaaaaccg gtctggcttg
     2341 agcaggtaca cggcaaagat gtgcttaagc ttactggcga accttatgcc tcaaaacgag
     2401 cagatgcctc ttatagcaac acgccgggca cggtttgcgc agtgatgact gccgactgcc
     2461 tccccgtgct gttttgcaat cgagcgggaa cggaagtcgc cgccgctcat gctggctggc
     2521 gtggactgtg ctcaggcgtg ctggaagaga cggtttcctg ttttgctgat aatccggaaa
     2581 atattctcgc ctggttaggg ccggcaattg gtccacgcgc gtttgaagtg ggggcggagg
     2641 ttcgcgaggc gtttatggca gtagacgcca aagcgagtac agctttcatt cagcatggtg
     2701 ataagtatct ggcggatatt tatctccttg cccggcagcg tctggcgaac gtcggtgtcg
     2761 aacaaatttt cggcggtgac cgttgtacat atacggaaaa tgagactttc ttctcttatc
     2821 gtcgcgacaa gaccaccggt cgtatggcaa gtttcatttg gctgatataa cctaaagaat
     2881 caagacgatc cggtacgcgt gattttcttt tcacattaat ctggtcaata accttgaata
     2941 attgagggat gacctcattt aatctccagt agcaactttg atccgttatg ggaggagtta
     3001 tgcgtctgga tcgtcttact aataaattcc agcttgctct tgccgatgcc caatcacttg
     3061 cactcgggca cgacaaccaa ttcatcgaac cacttcattt aatgagcgcc ctgctgaatc
     3121 aggaaggggg ttcggttagt cctttattaa catccgctgg cattaatgct ggccagttgc
     3181 gcacagatat caatcaggca ttaaatcgtt taccacaggt tgaaggtact ggtggtgatg
     3241 tccagccatc acaggatctg gtgcgcgttc ttaatctttg cgacaagctg gcgcaaaaac
     3301 gtggtgataa ctttatctcg tcagaactgt tcgttctggc ggcacttgag tctcgcggca
     3361 cgctggccga catcctgaaa gcagcagggg cgaccaccgc caacattact caagcgattg
     3421 aacaaatgcg tggaggtgaa agcgtgaacg atcaaggtgc tgaagaccaa cgtcaggctt
     3481 tgaaaaaata taccatcgac cttaccgaac gagccgaaca gggcaaactc gatccggtga
     3541 ttggtcgtga tgaagaaatt cgccgtacca ttcaggtgct gcaacgtcgt actaaaaata
     3601 acccggtact gattggtgaa cccggtgtcg gtaaaactgc catcgttgaa ggtctggcgc
     3661 agcgtattat caacggcgaa gtgccggaag ggttgaaagg ccgccgcgta ctggcgctgg
     3721 atatgggcgc gctggtggct ggggcgaaat atcgcggtga gtttgaagaa cgtttaaaag
     3781 gcgtgcttaa cgatcttgcc aaacaggaag gcaacgtcat cctgtttatc gacgaattac
     3841 ataccatggt cggtgcgggt aaagccgatg gcgcaatgga cgccggaaac atgctgaaac
     3901 cggcgctggc gcgtggtgaa ttgcactgcg taggtgccac gacgcttgac gaatatcgcc
     3961 agtacatcga aaaagatgct gcgctggagc gtcgtttcca gaaagtgttt gttgccgagc
     4021 cttctgttga agacaccatt gctattctgc gtggcttaaa agaacgttac gaactgcacc
     4081 accatgtgca aattactgac ccggcaattg ttgcagcggc gacgttgtct catcgctaca
     4141 ttgctgaccg tcagctgcca gataaagcca tcgacctgat cgatgaggca gcatccagca
     4201 ttcgtatgca gattgactca aaaccagaag aactcgaccg actcgatcgc cgtatcattc
     4261 agctcaaact ggaacaacag gcgttaatga aagagtctga tgaagccagt aaaaaacgcc
     4321 tggatatgct caacgaagaa ctgagcgaca aagaacgtca gtactccgag ttagaagaag
     4381 agtggaaagc agagaaagca tcgctttctg gtacacagac cattaaagcg gaactggaac
     4441 aggcgaaaat cgctattgaa caggctcgcc gtgtggggga cctggcgcgg atgtctgaac
     4501 tgcaatacgg caaaatcccg gaactggaaa agcaactgga agccgcaacg cagctcgaag
     4561 gcaaaactat gcgtctgttg cgtaataaag tgaccgatgc cgaaattgct gaagtgctgg
     4621 cgcgttggac ggggattccg gtttctcgca tgatggaaag cgagcgcgaa aaactgctgc
     4681 gtatggagca agaactgcac catcgcgtta ttggtcagaa cgaagcggtt gatgcggtat
     4741 ctaacgctat tcgtcgtagc cgtgcggggc tggcggatcc aaatcgcccg attggttcat
     4801 tcctgttcct cggcccaacc ggtgtgggga aaactgagct ttgtaaggcg ctagcgaact
     4861 ttatgtttga tagcgacgag gcgatggtcc gtatcgatat gtccgagttt atggagaaac
     4921 actcggtgtc tcgtctggtt ggtgcgcctc cgggatatgt cggttatgaa gaaggtggct
     4981 acctgaccga agctgtgcgt cgtcgtccgt attccgtcat cctgctggat gaagtggaaa
     5041 aagcgcatcc ggatgtcttc aacattctgt tgcaggtact ggatgatggg cgtctgactg
     5101 acgggcaggg gagaacggtc gacttccgta atacggttgt cattatgacc tctaacctcg
     5161 gttccgattt gattcaggaa cgcttcggtg aactggatta tgcgcacatg aaagagctgg
     5221 tgctcggtgt ggtaagccat aacttccgtc cggaattcat taaccgtatc gatgaagtgg
     5281 tggtcttcca tccgctgggt gaacagcaca ttgcgtcgat tgcgcagatt cagttgaaac
     5341 gtctgtacaa acgtctggaa gaacgtggtt atgaaatcca catttctgac gaggcgctga
     5401 aactgctgag cgagaacggt tacgatccgg tctatggtgc acgtccgctg aaacgtgcta
     5461 tccagcagca gatcgaaaac ccgctggcac agcaaatact gtctggtgaa ttggttccgg
     5521 gtaaagttat tcgcctggaa gttaatgaag accggattgt cgccgtccag taatgataaa
     5581 acgagccctt cggggctcgt ttttgtctat aaattagacg gaaaagacta tgtttaagat
     5641 gttttgcctg aaaaatgagc gaacgataaa gtttttatat tttttgcttg tcaggccgga
     5701 ataactccct ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccgggtta
     5761 gcggggttct cctgagaatt ccggcagaga aagcaaaaac aaatgcttga ctctgtagcg
     5821 ggaaagcgta ttatgcacac cccgcgccgc tgagaaaaaa gcgaagcgg
//
LOCUS       DAZKJR010000225         5862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKJR010000225 DAZKJR010000000
VERSION     DAZKJR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.226"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..704)
                     /locus_tag="ACHOWS_004894"
     CDS             complement(<1..704)
                     /locus_tag="ACHOWS_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFQ0050353.1"
                     /translation="MQVNVAETLFRTIDTATQSQMANMNTVMSVMGIVIGGIWMTYIL
                     MKSLYWYFEGLTTVIQDAFFTIFKAFAIMMASLTISWYTSTVIPPVLEFPLWLANTIA
                     GVNNDSSNLVDMVINSYISGLENLVDKMKFGFSVSSIKSMVTVGVAVVLYLLSGLPFL
                     GVAIATLLTLKVATNLILIIGPMFIACLAFPKVAHWFWGWVGVLGGFVLTQALFAITL
                     GMAISFINSNMINNGD"
     gene            complement(730..1128)
                     /locus_tag="ACHOWS_004895"
     CDS             complement(730..1128)
                     /locus_tag="ACHOWS_004895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050354.1"
                     /translation="MKIILKLCFLMLLLSGCNDKAKDFIGGWTQESNEEYPVYITINY
                     DSGIYHVDVKKLDTYLVEKKKADAFDDFMWGKKSKNESENLTFDDCYKVFNFEAKAIS
                     SSVLEGKGISVRMDGDRLIFDGKRFVRQKS"
     gene            complement(1154..1804)
                     /locus_tag="ACHOWS_004896"
     CDS             complement(1154..1804)
                     /locus_tag="ACHOWS_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFQ0050355.1"
                     /translation="MIRKSCIAACLSITLCSLSYAGGVPTVSIAELTQLVVNAQQQAQ
                     EALAQLNKAKEAIRQAKDQYDHYKSILQGNDKLGAFLNDPYINKLLPMKDWQDIYNQT
                     QDLTDLRKRYGITGYEPDVQKLFDKLLAQVDVYEKQYDATVTRVNHAEELRNKLNSVQ
                     TPKDREQLALRYQQEMLELENQRMQLENTRYLMEQKERIENKKNEEDFYNFMWGRS"
     gene            complement(1874..4408)
                     /locus_tag="ACHOWS_004897"
     CDS             complement(1874..4408)
                     /locus_tag="ACHOWS_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFQ0050356.1"
                     /translation="MQDALFKNAFSPESNAKVPWLGHHVAPEICTLGTSHILSLIRFS
                     GVPHDTRDQAMLNKEFMYLARCFKALGKQEGNNLKLQTYTFKRRIHLTHQYNLELPVF
                     QDLVDCWVKPFRNGTFRQVGYSMALILKFRDLDDGINRMRELLSLCREMLGDFGVSVM
                     ELEERDGKLYSQIGRFYSFILNGEEKDIPVTDTRLGDSIIDSETGFGAYDYVENRPSY
                     GKRRFASTYDLRDYPESKSYPGMWDEVLDEQYDFCMVQSFLFEDRNRMKRRLKTQMSD
                     LSSTEGQSSETEELADSVQEVTQGKKVFGEYYASLIVFGDTPEEAVDNGARMQSLLMA
                     RDTGFVRSRSTNYVTWLTLFPGYADVIYSMPKSTENLACGFSLHATPCGKADGNPPGD
                     GTALMPVMTENGGMYFLNAHASPLGKNCRGEKYPGHKTTIAMTGAGKTTVESLELVFF
                     SRWNPMIFSLDYNNSLENVLRALGTRYFNIKPGEFTWIQPFQMEDSVELRQYLFDVLV
                     QCAGGADSTEESKIQMSIDAVLKHKNVESRSFSLLLQSIPDTGPGGLRERLSKWCRDD
                     GNGRRGQYYWILDSPRNDFDPTLFRRLGFDCTKILNMEFATRHPQAMEVLLNTFFFLK
                     RRMHENEPGSLLINSIAEFWAPLMFESTANSIMEILHAGRTRGEMVMMDTQVPEYVLN
                     NERGPSVIQQTITQEWMANEKATSDSYSKFSVTSKEFSKIEEFSAYSRKVLVKQGGAS
                     VVLKVDMQDKLKYWLPLLSSDYANIPVAKRVREALGTDDPAVWVAPFLDEMVAIGVRK
                     ELAEKYPEQDVNDPQVWEPAFIEVMKQLERPVSMKLTTINNMNSKS"
     gene            complement(4365..4736)
                     /locus_tag="ACHOWS_004898"
     CDS             complement(4365..4736)
                     /locus_tag="ACHOWS_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFQ0050357.1"
                     /translation="MGETNEILEFDTNNAMNRVAMFAGIPVFALLFLVVIGCILTFSS
                     VWMWGWWGLIVAIPFILAIVGLRIICERDDKAFRRFRFQLRRWKMNRKYGRHLLLTSR
                     NPRWRDKYARRVIQKRILTGK"
     gene            complement(4736..5077)
                     /locus_tag="ACHOWS_004899"
     CDS             complement(4736..5077)
                     /locus_tag="ACHOWS_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050358.1"
                     /translation="MKEIMKKMIGLFSIPSRYIALLFVFIPVYAHAAWYDPIVKFVKE
                     FQTGIIIVGGAVAVGSLVYVGVCWIISRMSGNMETTAMDYVKHIGVIGAVGGAVAAAT
                     WAYQLWGGTIS"
     gene            complement(5081..5692)
                     /locus_tag="ACHOWS_004900"
     CDS             complement(5081..5692)
                     /locus_tag="ACHOWS_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFQ0050359.1"
                     /translation="MSILTTGAFLALAAQCAPNVHPDTSLDVVRVESGLHQYAIAEII
                     PKNERVAEGKSFISHYPESKDEAVKIIRRIEERKRNYSVGLMQINSSNFKKLRVTATE
                     LLEPCSNLKAYQYIITDCYLRGGSLTNALSCYYSGDFITGHKVESELNNTSYIRRIGL
                     LDNNYVVPGTREKINDIKDKTHDDIIFESWDILREYPKQLKRF"
BASE COUNT         1809 a         1374 c          888 g         1791 t
ORIGIN      
        1 aagtccccat tattaatcat attgctatta ataaatgaaa tagccattcc aagagtaata
       61 gcaaaaaggg cctgtgtaag aacaaatcct cctaaaactc ccacccatcc ccaaaaccaa
      121 tgagctactt taggaaaagc taaacatgca ataaacattg gaccaatgat aagaattaga
      181 tttgttgcga ctttaagcgt cagcaatgtt gctatagcca cccctaaaaa aggcaatcca
      241 cttagtaaat ataaaacaac agcaacaccg acagtaacca tactttttat tgatgataca
      301 gaaaatccaa atttcatttt atcaaccaga ttttccagtc ctgatatata tgaatttatt
      361 accatatcaa ctaaatttga cgaatcattg ttaactcctg caattgtatt agccaaccat
      421 aaagggaatt ctaaaactgg aggaataact gttgatgtat accatgatat agtcaatgat
      481 gccatcatga ttgcaaatgc tttaaatatt gtaaaaaatg cgtcctgtat tactgttgtt
      541 aagccctcaa aataccaata taatgacttc attaatatat atgtcatcca tataccgcct
      601 atcacaatcc ccataacaga catgacagta ttcatatttg ccatctgaga ttgtgtcgcg
      661 gtatcaattg ttctaaacag cgtttccgcg acatttacct gcatataaaa atcctttagc
      721 tatgtcacat tatgattttt gccttacaaa tctttttcca tcaaaaatta atctatcacc
      781 atccatcctt acagatatac ccttaccttc taataccgaa ctacttattg ctttagcttc
      841 aaaattgaac actttataac aatcatcaaa tgttaaattt tcagattcat tcttactttt
      901 tttcccccac atgaaatcat caaatgcatc agcttttttc ttttctacca gataagtatc
      961 tagtttttta acatccacat gataaattcc gctatcatag tttatggtta tatatactgg
     1021 atattcttca tttgattcct gagtccaccc tcctataaag tcttttgctt tatcattaca
     1081 tccagacaaa agcaacatta aaaagcacaa tttcaaaata attttcatat attcctcccg
     1141 ttatataata tgattatgag cgaccccaca taaaattata aaaatcctcc tcattctttt
     1201 tattttcaat tcgttctttt tgctccataa gatagcgcgt attttccagt tgcattctct
     1261 gattttcaag ctctaacatt tcctgttgat atcttaatgc taactgctcc ctatcctttg
     1321 gagtctgaac tgaatttagt ttatttctca actcttcagc atggtttact cgtgtgactg
     1381 ttgcatcata ttgtttttca tatacatcaa cttgagcaag taacttatca aacaattttt
     1441 gaacatcagg ttcatacccg gtaataccat aacgctttct taaatcagtt aaatcttggg
     1501 tttgattata tatatcctgc caatctttca ttggcagtaa tttatttata tatggatcat
     1561 tcagaaatgc ccccaattta tcgtttccct gaagaatcga tttataatga tcgtactgat
     1621 cttttgcctg tcgaattgcc tcctttgctt tattaagctg cgccaacgct tcctgcgcct
     1681 gttgctgtgc atttacaact aattgcgtta actctgcaat gcttaccgtt ggcactccac
     1741 ctgcataact tagagaacat aacgttatag agagacacgc ggctatacat gatttacgta
     1801 tcatatagaa ctcctgtgtt tatcaggaat gaacgttttc gttaaacatc aaagcattcc
     1861 tgatagcata aaatcaagac tttgagttca tattattgat ggttgtcaac ttcatagaaa
     1921 ctggacgttc cagttgcttc atcacttcaa taaatgcagg ctcccatact tgaggatcat
     1981 tgacatcttg ttcaggatac ttttccgcaa gctctttcct tactccaata gccaccatct
     2041 catccagaaa tggggccacc catacagccg gatcatctgt acctaacgct tccctaactc
     2101 gtttagctac aggtatattg gcatagtcag atgacaataa aggcaaccaa tatttcagct
     2161 tatcctgcat atccaccttc agaacgacag atgcacctcc ctgttttacc agaaccttac
     2221 gtgaataagc cgaaaactcc tctattttac tgaactcctt agaagttaca gaaaatttac
     2281 tgtaactgtc tgaggtagct ttttcatttg ccatccattc ctgcgtaatt gtctgctgaa
     2341 taactgaagg accacgctcg ttattcagaa catattcagg aacctgagta tccatcataa
     2401 ccatctcacc acgggtccgc cctgcatgta aaatttccat aatggaatta gctgtactct
     2461 caaacattag aggtgcccaa aattccgcta tactgtttat tagtaagctg cctggttcat
     2521 tctcatgcat tcttctcttc aggaaaaaga aggtatttaa taatacttcc attgcctgag
     2581 gatgacgtgt agcaaactcc atgttaagaa tttttgtaca atcaaatcca agacggcgaa
     2641 acaacgtagg gtcgaaatca tttctgggac tgtccaagat ccagtaatac tgtccacgtc
     2701 ttccattccc atcatcacga caccactttg ataatcgttc cctgagtcct ccaggaccgg
     2761 tatcaggtat agactgtaat aacagtgaaa aactacgact ttctacattt ttatgcttca
     2821 gaacagcatc aatcgacatc tgtattttag attcttcagt gctgtcagca cctccggcac
     2881 actgaacaag aacatcaaac agatattgac gtagttcaac cgaatcttcc atttgaaacg
     2941 gttgtatcca ggtaaattca ccaggtttta tattaaaata acgggttcca agagcacgga
     3001 gtacgttctc cagcgaatta ttgtaatcca gagaaaatat cataggattc cagcgactaa
     3061 aaaacactaa ttcaaggctc tcaaccgttg ttttacctgc cccagtcata gcaattgtcg
     3121 ttttatgccc tggatacttc tcacctctac agttcttccc taaaggactg gcgtgtgcat
     3181 tcagaaaata cattcccccg ttttccgtca tcactggcat caacgctgtt ccgtctccag
     3241 gaggattccc atcggctttc ccacacggag tcgcgtgtag ggaaaatcca caggccaagt
     3301 tttctgtaga ttttggcatc gaataaatca catccgcata accaggaaat agcgtcagcc
     3361 aggttacata atttgtactt cgggaacgaa caaagccggt atcgcgcgcc attaacaacg
     3421 attgcattct tgctccgtta tcaacagctt cttcaggtgt atctccaaat acaatcaggc
     3481 tcgcataata ttcaccaaaa acttttttcc cctgggtaac ttcctgaaca ctatcagcaa
     3541 gttcttcagt ttcactggat tgtccttccg tagacgataa atccgacatc tgagttttca
     3601 ggcgacgttt cattcggtta cgatcttcaa aaagaaaaga ttgaaccata caaaagtcat
     3661 actgttcatc cagcacttca tcccacattc ctgggtagga tttgctttct ggataatccc
     3721 ttagatcata agtagaagca aaccggcgct ttccatacga cgggcggttt tccacataat
     3781 cgtaagcgcc aaatcctgtt tcactgtcaa taatcgagtc tcccaggcgg gtgtcagtaa
     3841 caggaatatc tttctcttca ccgttcagaa taaacgagta aaagcggcca atctgcgaat
     3901 acagttttcc gtcccgctcc tccagctcca taacagaaac accaaaatcc cccagcatct
     3961 ctctacataa agataacaat tcccgcattc ggttaatacc atcgtccaga tcccgaaact
     4021 taaggataag ggccatagaa tatccaactt gccggaatgt cccattacga aatggtttta
     4081 cccagcaatc aaccaaatcc tgaaaaacag gtaattcaag attatactga tgtgtgagat
     4141 gtatacgacg cttaaaagtg tacgtctgca atttcaggtt attcccttcc tgttttccca
     4201 aagctttaaa acatctggca agatacataa actctttatt tagcatagcc tgatctcgcg
     4261 tatcatgagg aacaccagaa aaacggatca aactgagaat atgagacgtg cccagagtac
     4321 aaatttcagg tgcaacatgg tgccccaacc agggcacctt agcattactt tccggtgaga
     4381 atgcgttttt gaataacgcg tcttgcatat ttatctctcc atcggggatt tctggaagtc
     4441 aacagtaagt gacgaccata tttacgattc attttccagc ggcgtaattg aaaacgaaaa
     4501 cgacgaaacg ctttgtcatc acgttcacaa ataatacgca atcctacgat tgccagaata
     4561 aaagggatgg cgacaattaa tccccaccat ccccacatcc atacagaaga aaatgtcaaa
     4621 atacagccaa taacaaccaa aaaaagaagt gcaaaaacag ggatccctgc aaacatagcc
     4681 accctgttca tcgcgttatt agtgtcaaac tcgagaattt cgtttgtctc tcccatcagc
     4741 taattgttcc tccccacaac tggtacgccc aggtagctgc ggcaacagcg ccaccaacag
     4801 caccaataac accaatatgt ttcacataat ccattgccgt tgtttccata ttcccggaca
     4861 tacgagaaat aatccaacag actcccacgt agacaagaga accaacagca acagctcccc
     4921 cgacaataat aattcctgtc tgaaactcct taacaaattt aactattgga tcataccatg
     4981 ctgcatgagc atatacagga ataaaaacaa ataataacgc aatataacga gatggtatac
     5041 taaacagtcc tatcatcttt ttcattattt ctttcattaa ttaaaacctc tttaattgtt
     5101 ttggatattc cctgagaata tcccatgatt caaaaataat atcatcatga gttttatctt
     5161 ttatgtcatt aatcttttcc ctcgttccag gaacaacata attattatct aataatccta
     5221 tccttcgaat ataacttgta ttatttaatt ctgattcaac cttgtggcct gtaataaaat
     5281 ccccagaata ataacaactt aacgcgttag taaggctacc tcctcgcaaa taacaatcgg
     5341 taataatgta ttgatacgct ttcagattag agcacggctc caacaattca gtagctgtca
     5401 ctctaagttt tttgaaattt gaactgttaa tctgcattaa tccaacagaa taatttcgtt
     5461 tccgttcctc aatcctcctg attattttca ctgcttcatc tttactctcc gggtaatgac
     5521 ttataaagct tttaccctct gcaacccttt catttttagg tataatttcc gctatagcat
     5581 attgatgtaa ccccgactca acacgaacca catcaagaga tgtatctgga tgaacgtttg
     5641 gggcacactg tgcagccaga gcgagaaatg ccccggtagt cagtatactc atcagcgcat
     5701 tttttctcgt gcattagcaa cataattaat aacttctttc ctgattcctt cgctctgtaa
     5761 tgcacagttt aaaagacaat gaacaatata tgttggcttt acaagtaatt gacactcatc
     5821 atttattcct ttaataaatt ccattaactg cgcttttgta tc
//
LOCUS       DAZKJR010000226         5828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKJR010000226 DAZKJR010000000
VERSION     DAZKJR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.227"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHOWS_004901"
     CDS             complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHOWS_004901"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFQ0050360.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHOWS_004902"
     CDS             complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHOWS_004902"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFQ0050361.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVNLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEEHHE"
     gene            complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHOWS_004903"
     CDS             complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHOWS_004903"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4048..4050),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFQ0050362.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4867..5748
                     /gene="yddG"
                     /locus_tag="ACHOWS_004904"
     CDS             4867..5748
                     /gene="yddG"
                     /locus_tag="ACHOWS_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFQ0050363.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LSFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         1325 a         1618 c         1435 g         1450 t
ORIGIN      
        1 ggcgcttctt aaagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca
       61 ctctcttttt tcgcttccgc cttctcgatt tcacgatacc agcgcggatg gtgtttcttc
      121 gcccagcgac ggctcacctt cccttcgatc atccctttaa tcgatccttt cacccagaat
      181 gccatataca tatggatcag gatggcgtgg atcaggatga tacccgcagc cgcgtgaatc
      241 agcaggctgt agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata
      301 atcaccccgg tcaccagcag cacgaaaatc atgctcatga tcgaccagaa catcatcttc
      361 tgcccggcgt tgtacttacc aacatccgcc actttgtgct cattgccttt taatacttcg
      421 acaatgttca acagccacgg aatatccttc ttatccggga tattgtgatg tacaaaacgc
      481 acaaacataa acatcagcgc gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt
      541 cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa
      601 atcccggaca gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca
      661 ataaacttgg tgcgcacaat cattttcgac ttactcatga tgctcctcct cgtcatcgtc
      721 cacttcctta ttcgggccaa taccgatgta gtggaaaatc aacccggcaa aagtggcgat
      781 aaagccagcc gctgccagcg gtttcaacgc gcctttccac aagttcaccg atgtgtcgat
      841 cttcggatct ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcaacacgta
      901 catgacgtgc gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg
      961 cgctttcagt ttcgctacgc gctgttccgc cagctccagc atctccttct tggtgccgaa
     1021 gtggatagcc ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg
     1081 atcgacgcag agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt
     1141 aaacggacac ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcaac
     1201 aatcccgtta gcgtactgaa tgattgcacc agcagacggg cacgccttca ggcagcccgg
     1261 atcttcacag tgcatacagc catctttacg gatcagccac tccagcttgc cgttctgttc
     1321 ggtttcgcta aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta
     1381 aaccccgacg cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg
     1441 acaggcttta cagccgatac aggtggaaac gtcgataagt ttcgcgactt ctgctttgta
     1501 atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatgtcctg
     1561 cgtttccata gccatctgtt cgccccctta cgccttctcg atgtttacta agaacgcttt
     1621 atattccggc gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagcgatata
     1681 acctttacgc gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg
     1741 ctgaccgttt acattcagcg gtttcagacg gcgcgttacc acagccaccg cgcggataaa
     1801 gccacgcttg ctggagacag tgacacgatc gccattggca atgcctttcg ccgccgccag
     1861 cgtttcgctg atttccacaa actgttccgg ctgagcaatt gcgttgagca atgcgtgctt
     1921 ggtccaggtg tggaagtgct ccgtcagacg ataggtcgta cccacatacg ggaactgctc
     1981 ttttttaccc atccgcagtg cgtcttgttc atacagacga acaaccgggt tagacaccac
     2041 gttcggatgc aacgggttag tgcccagcgg cgtttcaatt ggctcatagt gttccgggaa
     2101 cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcatgat
     2161 aaacggcccg gttggcgtac ccggtgcggc attgccgaag tccggaatat cgttacccgt
     2221 ccacttgcta ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccgttgat
     2281 atccgccgaa gcacggttgt agagtacgcg acggttgagc ggccacgccc aggcccatcc
     2341 cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct ggttgccctg
     2401 ctcggtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag
     2461 atgcgcaaag ctactcagca actgaccttt cttcgcaatc agcacgccat tagcgtcata
     2521 gagatcttcc agcgcatagc cgttgttctc tttcgccact tcgtcagact gcggttcgtg
     2581 cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac caccctcggc
     2641 ctggtacagc tcgcgcagat gatggtagat acccgccaga atttcgccgt cgttacgtgc
     2701 ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat
     2761 agaaccatct tcttcagcaa agcaggtcga aggcagacgg aatacttcag tctgaataga
     2821 tgccggatcg acatcgttcg actcgccgtg gttctgccag aaggtagagg tttcagtcac
     2881 cagcggatcg ataaccacca tgtacttcag tttgctcagg cagctcacca ctttgttttt
     2941 gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga ctttaccttc
     3001 atccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc
     3061 gtagccccag ttgttctctt tctgcgcggc atcgccgtag aaagatttca tcaggctaac
     3121 gaaaaacttc ggatagttgc tccagtagtt cacctgatca gccagcgtcg ctttcggcgt
     3181 gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc
     3241 tggcaggctg gtagagagca ggcctaagtc agtcaggccc tgaatgttgg agtgaccacg
     3301 caatgcgttc acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc
     3361 catcgtacgg atgttctgcg cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa
     3421 ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc
     3481 ggcttttggc gtaccacaga tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc
     3541 tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc
     3601 gttttcatcg aactgatagt tccaggacga tttatcgtat tgccgttttt cagcgtcgta
     3661 gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaggc tggcattggt
     3721 gtaatgctta acgtattcgg cattgatttt gttgttttcg atcaggtagc gcaaaacgcc
     3781 agacaggaac gtaatgtccg taccggaacg aataggtgcg taaatatccg ctacagaagc
     3841 ggtacgcgta aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc
     3901 catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca tcaccattac
     3961 gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac caaatgttgg
     4021 agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ctgccagcat
     4081 cccgagggag cgggcaaatt tttgagtcag catcccggtt tcgttgctgg caccggaggc
     4141 acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat
     4201 aaagttagcg tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca
     4261 gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc gcagacggtt
     4321 ttcgctgttg acgtaatcca gcaaaccggc ccctttcggg cacagcgcac cacggcttac
     4381 cggatgatcc gggtcacctt caatgtgata aatcgcttct ctggcgtttt tcgcgccatc
     4441 acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat
     4501 ctctttcgcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa
     4561 gcccaatgcc gctaccgttg ttccagccat accgcccgcg cagattttaa aaaattgtct
     4621 gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac
     4681 agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa
     4741 tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag
     4801 gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca
     4861 gcgagcatga cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc
     4921 acgatggtag gattgattcg cggtgtcagt gaggggctcg gcccggtcgg cggcgcagct
     4981 gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg
     5041 cagatcccga aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctgt
     5101 ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt ggggatggtg
     5161 aactatctat ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc
     5221 aactggctga ttgtaccagg attattatta gccctggtcg gcgtctgctg ggtgttaggt
     5281 ggtgacaatg ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt
     5341 tatttcctgt cgtttattgg tgcgtttatc tgggcagcct actgcacagt aacgaataaa
     5401 tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc taacgggagc aagtctgtgg
     5461 gtttactatt ttctcacgcc acaaccagaa atggtattta gcacgccagt catgattaaa
     5521 ctcatctctg cggcatttac cttaggattt gcttatgctg catggaacgt cggtatatta
     5581 catggcaacg tcaccattat ggcggtaggt tcgtatttta cgcccgtcct ttcctcagcg
     5641 cttgcagccg tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg
     5701 gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggctaaaa taattgtcgg
     5761 gtctacggcc cggcagttaa tcagataaat tctgacaata ttaaaaaata aatttcaaat
     5821 accagaaa
//
LOCUS       DAZKJR010000227         5749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKJR010000227 DAZKJR010000000
VERSION     DAZKJR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.228"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(344..1303)
                     /gene="nrdF"
                     /locus_tag="ACHOWS_004905"
     CDS             complement(344..1303)
                     /gene="nrdF"
                     /locus_tag="ACHOWS_004905"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0050364.1"
                     /translation="MKLSRISAINWNKITDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1313..3457)
                     /gene="nrdE"
                     /locus_tag="ACHOWS_004906"
     CDS             complement(1313..3457)
                     /gene="nrdE"
                     /locus_tag="ACHOWS_004906"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0050365.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSSDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDIERVYGKPFADVAISEHYAEL
                     VANERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSSITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3430..3840)
                     /gene="nrdI"
                     /locus_tag="ACHOWS_004907"
     CDS             complement(3430..3840)
                     /gene="nrdI"
                     /locus_tag="ACHOWS_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0050366.1"
                     /translation="MSQIVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3837..4082)
                     /gene="nrdH"
                     /locus_tag="ACHOWS_004908"
     CDS             complement(3837..4082)
                     /gene="nrdH"
                     /locus_tag="ACHOWS_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0050367.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAANA"
     gene            complement(4329..4658)
                     /locus_tag="ACHOWS_004909"
     CDS             complement(4329..4658)
                     /locus_tag="ACHOWS_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFQ0050368.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4810..5154
                     /locus_tag="ACHOWS_004910"
     CDS             4810..5154
                     /locus_tag="ACHOWS_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFQ0050369.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5191..5640)
                     /gene="alaE"
                     /locus_tag="ACHOWS_004911"
     CDS             complement(5191..5640)
                     /gene="alaE"
                     /locus_tag="ACHOWS_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFQ0050370.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1446 a         1411 c         1435 g         1457 t
ORIGIN      
        1 ttcctgttat ttgtttatgt cgatacatta cccaatggaa atagtcactt ttttctaccc
       61 taacatactg agaatctgag gcaacccctg atggcaaatg tggcatgaat attgatgtga
      121 gcaaatcgcc ggaatttccc atgatataag ggctgagagc aaatcgagaa aagttgatat
      181 tttttgtcaa agatagcaaa aaaacgcctg aatattgtgg tttcaggcga ataatgcaac
      241 agagtatcag tgtagatcac cacaaattat ttgcgtgata taaagctatt tggcgggaat
      301 tatttccctg cagcgggtag tgatattttt gaaaataacc ccctcagaaa ttccagtctt
      361 catcttctgt ttcgaccgct tttcccatca catatgaaga gcctgaaccg gaaaagaaat
      421 cgtgattttc atcggcattc ggcgaaagcg cggcgaggat tgccggattc acttccgcca
      481 tttctgcggg aaatagcggc tcgtagccca gattcatcaa cgccttattg gcgttgtaac
      541 agagaaacgc tttcacatcg tcagcccacg gggtttcggc gtacagctca tcggtgtatt
      601 gcaactcgtt gtcatagagt tccagcaaca aatcgaaggc gaaactcttc aattcttcac
      661 gtcgtgcctg ggagatcttt tccaggtttt tctgatattt atagccgatg tagtaaccgt
      721 ggactgcttc atcgcggata atcagacgga tcaggtctgc ggtattggtt agctttccgc
      781 ggctggaaaa atacatcggc agccagaaac cggaatagaa caaaaaagat tcaagaaaca
      841 cactggcgat tttctttttc agcggatcat caccgcgata atgttgctga ataatctgtg
      901 cttttcgctg caacggcgcg ttttcttcac tccaggcgta ggcggcatcg acatctttgg
      961 tctggcacag cgtcgagaaa atcgaactgt aagagcgggc atgaaccgcc tccataaagc
     1021 tgatattcga taatactgcc tcttcatgag gcgtgagcgc atcgggcatc agcgaaggcg
     1081 cgccgataac attttgcagc gtgtcgagca gtgtcaggcc agtaaaaacg cgcatcgtca
     1141 gttgttgttc tacgacactt aatgtctgcc aggcgggaat atcgttcgat agtggcacct
     1201 tttctggtag ccagaaattg ctggtcaggc ggttccacac ctccagatct ttatcgtctg
     1261 ttatcttgtt ccagttgatg gcgctgatac gtgagagttt catagatatt ccttaaagtg
     1321 cacaggagac gcagccttca atttcagtgc cttccagcgc catctgacgc aggcggatgt
     1381 agtagagcgt tttgatacct ttgcgccagg cgtaaatctg cgctttgttg atatcgcgag
     1441 ttgtggcggt atcggggaaa aacagtgtca gcgacagccc ctgatcgaca tggcgagtcg
     1501 cttcggcgta ggtgtcgatg atcttttctg cgcctatttc gtaagcgtcc tgatacagcg
     1561 ccagattctc gttagtcata aacggggcag gatagtaaac gcgtcctgtt ttgccctctt
     1621 tgcgtatctc tactttcgcc acaatcggat gaatactcga cgtggcatgg ttgatataag
     1681 agatagaacc ggttggcggc accgcctgga gattttggtt gtatatgcca tagcgcatca
     1741 cgtcgtcgcg cagttgcgcc cacatttcgc gggtaggtaa cgtgatgctg ctacgagcaa
     1801 acaactcgcc aactttcgcc gttttcggtt gccagttact ttgcagatat tggctaaaat
     1861 attcaccgct ggcatagcgc gactgtttga acccggcaaa ggtttcgccg cgttcgcgcg
     1921 ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggt atagaaatag agattggtga
     1981 aatcgagtgc ttccggcgat ccataagcga tgccttctcg cgccagataa ccgtgtaaat
     2041 tcatctgccc cagtccgatg gcgtgtgagg cggcatttcc ggcttcgatg gacggcacgc
     2101 tgcggatatg actcatatct gacaccgccg ttaaaccgcg cacggcagtc tctaccgtgc
     2161 gggcaaagtc gggggaatcc atggtgtggg caatattcaa cgaacctaaa ttgcaggaaa
     2221 tatcatggcc tgtgcgggca tagtcgagat tctcgtcata ctctgaggcg ctgttaacct
     2281 gcaaaatttc tgagcagaga ttgctcatat ttatgcgccc ggcgataggg ttagcacggt
     2341 taaccgtgtc ttcatacatg atgtacggat agccggactc aaactggatt tctgccagtc
     2401 gctggaagaa atcacgggcg ttgatgtatt ttttgcggat gcgttcattg gcaaccagtt
     2461 cggcatagtg ttcactgatg gcgacatcgg caaatggctt gccataaact cgctctatgt
     2521 catagggcga aaacagcgcc atctgcgcat tctcttttgc cagatggaaa gtgatatccg
     2581 ggatcaccac gcccagcgac agtgttttaa tgcggatttt ttcgtcggca ttttcccgtt
     2641 ttgtatcgag aaaacgcaga atatcgggat gatgagcatg taaatagact gcgccagcac
     2701 cctgacgcgc gccgagctgg ttggcatagg aaaatgcgtc ttccagcatt ttcatcaccg
     2761 gaatcacgcc agaagattga ttttcaatac gtttaattgg cgcgcccgct tcccgcagat
     2821 tcgacagcaa aaatgcaacg ccgccgccgc gtttcgacag ttgcagtgcg gaatttactg
     2881 cccggccaat cgactccata ttgtcttcaa tacgcagcaa aaaacaggaa accagttcgc
     2941 cgcgctgctg cttaccacag ttgaggaatg tcggtgttgc aggctgaaag cgtcctgaca
     3001 gcatctcatc ggtcagttgc gtcgccagtg tttcatcgcc ttgtgccagc gtcagcgcca
     3061 ccatcgtgac tcgatcggca aaatcttcca gataacgctt accgtcgaat gtcttcaacg
     3121 tatagctggt gtaaaacttc catgctccga ggaatgtctg aaaacgaaaa ccgctggcgt
     3181 gtgcgtgggc aaacagcgta atgacaaaat cgctagagta gcgattaagg acactttcat
     3241 catagtaacc ttcgttaacc agccagttca ggcgctgctg ctggctactg aaggccacac
     3301 tgttcggacg cacatgcgtc gtcataaagg cgtctactgc ctggcgatct ttatcgaact
     3361 gaatgcgacc tgcgctatcg taaaggttaa gcatcgcatt cagcgcgtgg taatccatag
     3421 tttcctgcgt caggcgttct gcggttgtcg ttgccaaaat tcggttactc ctttacgaac
     3481 gttttcgata tcgctttgcg tacccatgag ttcaaagcgg tacagccatg gcacgccgca
     3541 tttccgggca atcacatctc cggcgcggcc atatgcctca ccaaagttgc gattacccga
     3601 agcaataacg ccgcgcagca acgcccggtt atgctcgtcg tttaaaaagc gaatgacctg
     3661 tcgtggcacc gcgccagccg taccgccgcc gccgtaagag ggcacgatca ggatgtaagg
     3721 ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg gcagccctaa
     3781 acgttcgata aaacgctgcg tattttcgga gctgctggaa aagtagacaa tctggctcat
     3841 gcattggccg cgtgtggtgc tggatgcaga cggttgatca tgtccgggcg gaaacctgac
     3901 cagctaagat cgcctgcaat cacgaccggc aactgacgaa agccctgagc acgcagcgct
     3961 tccgccgctt caggaacgcg atcgacatta atcatttcaa agtcaaagcc ccggttttcc
     4021 atcgcccttt tggtggcgtg gcactgaacg caatcgttac gagtgtaaat agtaatgcgc
     4081 atgattcgta tttccgttta aaatgaagat acggcgcgat gatacgcgtc gggttgtctc
     4141 tctgttgata cagaaatact agatgtagtt gaaaaaagat tcaaccacac aatatatagc
     4201 aaatagtggt cgaaattacc ctgggtatga gcgtgacggg gtagggggat ttttgtgatt
     4261 caccaggcaa aaagaaaccc cgaagccagg cttcggggtt aaagacgcgt atttattatc
     4321 atgttgcact acgatttgcg catgcttaac agtgcgccga taaaaatacc taccgcagct
     4381 gctgtaccca cgctacacca gggtctttca cgaacaaaag aatccgcgca gccaacggca
     4441 tcgcgcgcgg cttgctggac gcgagtacga ccatgcattc gcgctctggt ttctttcagt
     4501 agagcctgcg ctttactacg agcggcttcg gcttcccctt tggcgtcgct gccccaggac
     4561 ttcaatacag attcgagact atcagctaat tgattgacat cattctggat atcctgcacg
     4621 ccatcatcaa cgtcgttgcg gttcggtcgg ttaaacatat gatcccccat tagaattcga
     4681 tgtaattttc agtgtagacc ataattttct taactgaggt tcatcgcagc tttatggact
     4741 tttctgaaag cgttgtgaat gcgcaatcag gtaaggtagg cccgcggttg ataataacga
     4801 ggatcaaata tgtatttacg accagacgag gtggcgcgcg tacttgaaaa agtcggtttt
     4861 actgtcgatg tggtaaccca gaaggcttat ggttaccgcc gtggggaaaa ttatgtctat
     4921 gttaatcgcg aagcgcgaat gggacgtacc gccctggtga ttcatccaac attaaaagaa
     4981 cgtagttcaa cactggctga acccgcttcc gatattaaaa cttgcgatca ttatcaacaa
     5041 tttccgctct atttagcagg cgagcgacac gagcattacg gtatcccgca tggctttagt
     5101 tcgcgtgttg cgcttgaacg ttatttgaat ggtttatttg gcgaagccag ttaaagacgc
     5161 gactggcgat gccagtcgcg aaaagaaggg tcaggctttt acctgctggt aacggctgac
     5221 tttaaacagt cggcggcaat aatcgaggaa gtagccataa accgccccca tcaacatcga
     5281 aacaacgatg tttgaactga ccgccgccat aatctgatgc cagtctgcgc ccaccactaa
     5341 caagatcgcc acatacaccg gtgactggaa cgtcacataa gccatgatat ccgccagatt
     5401 ttttatccag cccgacgggc taactttgcg tgctgcgcgc ataaacagat cgcggtacat
     5461 accgtatggc catgcaatta agatgtttac cggaatcgct accaatctgg aataaaaaga
     5521 ctgttcgaag ctcattccgg agaggaaaac ttcaatacac atgttcacga cagaacagta
     5581 aacaaccatc gcgaacgtat ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat
     5641 gctaatgctc cttaataaaa gcgtaatggg atgtgaatgg agatgaaagc agtggatctc
     5701 attgccatct gcatttttgc atagcgtata tcgctggcta tgactaatc
//
LOCUS       DAZKJR010000228         5650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKJR010000228 DAZKJR010000000
VERSION     DAZKJR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.229"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            698..868
                     /locus_tag="ACHOWS_004912"
                     /pseudo
     CDS             698..868
                     /locus_tag="ACHOWS_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1084..1917)
                     /locus_tag="ACHOWS_004913"
     CDS             complement(1084..1917)
                     /locus_tag="ACHOWS_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0050371.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(1924..3261)
                     /locus_tag="ACHOWS_004914"
     CDS             complement(1924..3261)
                     /locus_tag="ACHOWS_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HFQ0050372.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPPSGASTLVWRQE"
     gene            complement(3359..4369)
                     /gene="iolG1"
                     /locus_tag="ACHOWS_004915"
     CDS             complement(3359..4369)
                     /gene="iolG1"
                     /locus_tag="ACHOWS_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HFQ0050373.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(4387..5292)
                     /gene="iolE"
                     /locus_tag="ACHOWS_004916"
     CDS             complement(4387..5292)
                     /gene="iolE"
                     /locus_tag="ACHOWS_004916"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFQ0050374.1"
                     /translation="MYSVKESIKWGIAPIGWRNDDLPEIGKDNTYKQILSDAALTGFS
                     GTEVGGCYPLDPTELNIELALRHLEIPGKWFSSFIIRDGIESVKKAFEQHCAYLQAVH
                     AYIAVVSEQTYSIQGIIDKCVYTEKPYFNDIEWQQLCSGLNELGMIANTYGLKLAFHH
                     HMGTGVQTLQEVDRLMENTDPQLVNLLFDTGHIYVSDGDVMSLLCKHFSRIKHVHFKD
                     VRDEKLKACRAGKKSFLNSFLDGMFTVPGDGNIDFKSVLAYLVENHYSGWIVVEAEQD
                     PNIYNPLEYAQKGKKHIDGLLGNYI"
BASE COUNT         1646 a         1385 c         1065 g         1554 t
ORIGIN      
        1 tttaactggt tgctgatata agtttttcac cacgcataaa cactgcccat gcgatcctgg
       61 caatcttatt tgccagctct atgatgacga tattcgttgg tcgacgtgcc tgctgggctt
      121 aaatctattc ccccggggat ccggctttat tgcaaccata aagctgcgag cccctatgta
      181 attcgctgac tcacacctca tttactgcgc gggattcaag cccaaaaccc catttaggtt
      241 tagtgagtcc ctgtcttacc tgtctttgtc atcacgttga ccaccagagc aacctttctg
      301 cattcaccca tttaagtaac caaaatgagt atcaggcctg atatatacag aactgaattc
      361 gttaattaat tattaatcag ttaaattttc cgttactggg atgaggtgta cacttggcct
      421 ctcccaaaat ccgcggaacc gcccctggcc cctattcagc atttcaggct ctaagttgag
      481 gactgaccgg attgacatag ggcctgctga cgccattcat tgactaccaa cagatctttc
      541 ttgttattta tagaaaacca ccagccagcc tagtggttca gtaaaacttc caactttgag
      601 gctgttgtca aaaccccttt ttgatttgtt aaaacacctt gcagtctacc aactgtaagc
      661 gtcaaacaag aaatcaaaag gggattccaa tgggtgaatg aaaaagggct taacgaacac
      721 ccgatggaac tgtaaatatc acatagtttt tgcgccaaaa taccgaagac agatgttcta
      781 cagagaaaag cgaagtgcaa gagcaccagg caatattttg agaaaactgt gtgagtggaa
      841 aagtgtacgg attctggaag ctgaatgctg agcggatcat atccatatgc ttgtgaattt
      901 acggggcgta agtaaggaag ttgaatgaaa atgtcagatc atatgcgcct gtcagccagg
      961 gcgaggcaga aaacgaatct ttaagacaca tctgaaaaac ctccggctat gccggaggag
     1021 atttattctg atcacctgct tattaccggg cacagcgaca gttcatttgc agttacgtca
     1081 gcgttaatcc gctaagaaag gtcgcgtttt ttccgctccg atagcaaaga cctcgctggc
     1141 tgccatttgc cagtaacctg ggttaaacaa ctccagtgaa cagatccctg tataaccttt
     1201 tttcaccagt tcatgggtga tcatgtacag tggtatcgca ccgttgcctg gaaacagacg
     1261 atgacaatga tccaacacag ctacaggtaa acggtcagca tcatcaatat gatagacaaa
     1321 gatcttttcc accggaacct gtcgcagcgt actcagatcc tgccattgat cgtacagata
     1381 aaggttaaag gcatctattg ccagaccaac gttactccga tcaactgcgt ccacgatctc
     1441 catcgccatc gccagactgc gtacgcaaca tcccgcagag ccgataggct caaaggccag
     1501 tcgcatctgg ctctctgccg ctaatgctga catctcacgc aaacgtttca ctgaatctct
     1561 taccgtgtca cgatctgaaa aatgagcacc ttcacgaatg gtcggcacta caaccaggca
     1621 atcaccacct acgacttccc ctgcataaca ggctaaaacc agttgttgcc gtatttccga
     1681 ccagcgcccg gcatcacaaa atgtgatgtc ttccagcgag ttgtagcccc atggcttaag
     1741 atggcttaca gaaaaaatat cggccagttc aaccagagtg tgttcctgca gccacgtctg
     1801 caacatatcc agacggatct caatatagtc atatccaaaa cgttccgcta ggaccagatc
     1861 cttctccagg ctggaattac gcagacaggt cgcttcattg aatcccagtt tcatcattca
     1921 gacttactcc tgccgccata ccaatgtact ggctccagaa ggcggcagcg ttaatgcgag
     1981 ttctctgtcg ccatcgacca cccggaaatg cctttctcgc gtatcgcgat tatataccac
     2041 taaaactcgc tcaccatcag gattaacaaa acccacatct tccagttgcg tatcataact
     2101 ggaagtaagc ataactttcg caccgggatg cacaaagcgg caaaagtgtc caattccgta
     2161 ccaagagtgg ttacaccaga gaatgtcatt caatgcatcg tactgcacag gagcttcgca
     2221 caaattactc tggtgatttg ggcctccatc actgtctaac agcaggttcc agtcgataaa
     2281 tccgctacag ccggatttaa aattaccaat catgtcatga atataggtat gccagtgacg
     2341 gatctggcca ccttcatcat tctccattgg tacacatcct tcggaaaaca gcagcttttt
     2401 attcggcaac ttccgggcca gatactgaat ttgcgaaaaa tggtcaccgg tataccagtg
     2461 gaaagccatc ccgttgatgc tatcgccatc cttttcgtca gcaaaggcac gttcagccca
     2521 gtcaatcaga ccatctttat catgatccca gatatagatc tctatttcat tcattccata
     2581 gcgggcgagt cgtgggcgca gatagtgaac agcaaaagta gtctcttctt ctacactata
     2641 cagacaggaa tcccagcttt ttatcgcaac tggttcattc tgcactgata gtgctttaac
     2701 actgatacca tggcgtctgt attcgagcag atagtcgata ataatatctg cccagtcagc
     2761 atagtattca taccgcaatt tcccacctcc gttcatatca taattagtct tcatgaatgc
     2821 cggtggactc cacggggaag ccattaattt tatttgtgga ttgatccgca atgccgcgga
     2881 aattagcggc attaaatgag attcatcacg ggaaaaggaa atccttccct gacgcaggtc
     2941 ttcactggaa tcaacatagg cataattgct cagagagaaa tcacagcttt ggatcggcat
     3001 ccgccccaaa ctatagttat gttcctgagg cgaaaaatag agattcaaaa attgcgcctt
     3061 tgtcttatca ctcatgctgt tgaaaacaac accagcactt tcagtgaagc tgccgccaaa
     3121 tccatcaatc tgttgtaacg agtggtttgg aaagacgctg attaattcac tacacgctgg
     3181 ttgacgaggc gaaaaaaaaa cctccagctc aaccataaat tgctgattcc agggatcgga
     3241 agtgattagt ctgcctttca tagagggatt ccagataata taggatgata aattgtgata
     3301 taccggacag gagcatacac ctagctatcc ggtatgaaat tatcacatcg cagaaagact
     3361 atttgtagaa gtcaggtttt gctggtaatt ccacaaactc ggtgtttcct gtttcctggg
     3421 acttcacaca ggcatcagcc gttaccgcag ccagataacc atcccatgat gttgggcctg
     3481 ccagaggtaa tcccgcattc agacggtcaa agaagtcctg gaactcaata tcataggcat
     3541 caataaagcg ttgtttccag tccaccaaaa tgtcagtact gtattttgcc tctttacgca
     3601 ccgcagcagt agccacggta ggtaattcca ccatcccttt ctcacaggtc acatcacaat
     3661 gaatatcata gccatactgg caattcacaa acacctcaac cactatatta ataccggagg
     3721 tcgtttccat caccaccacc tgcggatctc gtagagtagt caccaagctg gactgacgtg
     3781 ggaagtagac cttaactgtt ctataatctt cgttgagtaa ccagtgcata acatcgattt
     3841 catgaatcag cgtttcataa attgcttgtg gtgttttgta atccgatacc gtgcttacat
     3901 tataatgacg accatgaacc attaatggtt gaccaatttc tccgctatcg atgatgtttt
     3961 tcagctgtac gtagccttta tcgtagcgac gcataaaccc aacttgcacc attcgttttc
     4021 catttttacg ttcagctccg atcacacgct gacaatcatc tgctgtaacc gcaagcggct
     4081 tttcacagaa aacatattta ttggcattta acgctgcaac ggctacatcg gcatgtactt
     4141 cattcgaggc agtaattacc actacttcga catcttcatc attaatcaga tcgtgatagt
     4201 ctttatagtc ttttgctttg atctcatact tatccaaagc tgcttgcgct ctgccttcga
     4261 caatatcgca cacagcaaca acttctactc cagaaatagt gttagccaga cgtcttaagt
     4321 gatcggcacc aattttcccg atccccacaa tacctgcttt taaagtcatc ttattttcct
     4381 tttctatcag atataattac ccaataaacc atcaatatgc tttttacctt tttgtgcata
     4441 ttccaaagga ttatatatat tcgggtcctg ctcagcttca acgacaatcc agccggaata
     4501 atgattctca accaaataag ccaatacaga tttaaaatca atattcccat caccaggaac
     4561 agtaaacatt ccatcaagaa aagaattgag aaatgatttt tttccagccc gacatgcttt
     4621 tagtttttca tcccgaacat ctttaaagtg aacatgttta atacgggaaa aatgcttgca
     4681 caacagtgac atcacatctc catcagaaac ataaatatga ccagtatcaa acagcaaatt
     4741 cactaattgt ggatctgtgt tttccataag tcgatccact tcctgtaaag tttgtacgcc
     4801 agtgcccata tgatggtgga aagcaagttt aagcccatat gtattggcga tcatgcctaa
     4861 ctcgttcaat cctgagcata attgttgcca ttcgatatca ttgaagtaag gtttttccgt
     4921 atagacacat ttatcaataa ttccttgaat actatacgtt tgctcagaca caacagcgat
     4981 ataagcatgg acagcctgca aataagcgca atgttgttca aaagctttct tcactgattc
     5041 aataccatca cgaataataa aagatgagaa ccactttccg ggaatttcca gatggcgaag
     5101 tgctaactct atatttaatt cagtcgggtc cagaggataa catcccccca cttctgttcc
     5161 agaaaaacca gtaagtgctg catcacttaa tatctgctta taggtattgt ctttaccaat
     5221 ttcaggcaaa tcatcatttc gccatccgat tggcgcaatc ccccatttaa tactctcttt
     5281 tacactatac atattccaga ctcctgctta ataaaacttt ctcacatcct gttgcataag
     5341 taataatctt tggctgacaa tatgctactt tccacgtggc taaaaaactt tatctatcat
     5401 gagtacagaa gtgggaaaaa tgtgtcagga atcacaaaaa aaacacaacc ctttaatgat
     5461 tttgtctatg ttttagtcat gctgaaaaat cacacaatga ccgtaaaata aaaccgtcac
     5521 tttcccacta actctggcgt ggacagaatc atcccgtatg attcccaagt ttggtaaata
     5581 ttattaaatc aggatgtgca agctatgatt cagtcatact aggaaaaaag tttttttgca
     5641 gattcggttg
//
LOCUS       DAZKJR010000229         5647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKJR010000229 DAZKJR010000000
VERSION     DAZKJR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.230"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            414..639
                     /gene="ykgL"
                     /locus_tag="ACHOWS_004917"
                     /pseudo
     CDS             414..639
                     /gene="ykgL"
                     /locus_tag="ACHOWS_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(674..814)
                     /gene="ykgO"
                     /locus_tag="ACHOWS_004918"
     CDS             complement(674..814)
                     /gene="ykgO"
                     /locus_tag="ACHOWS_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFQ0050375.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(814..1077)
                     /gene="ykgM"
                     /locus_tag="ACHOWS_004919"
     CDS             complement(814..1077)
                     /gene="ykgM"
                     /locus_tag="ACHOWS_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0050376.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1442..1543)
                     /gene="ykgR"
                     /locus_tag="ACHOWS_004920"
     CDS             complement(1442..1543)
                     /gene="ykgR"
                     /locus_tag="ACHOWS_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFQ0050377.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2066..2266)
                     /locus_tag="ACHOWS_004921"
                     /pseudo
     CDS             complement(2066..2266)
                     /locus_tag="ACHOWS_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
     gene            2626..3519
                     /locus_tag="ACHOWS_004922"
     CDS             2626..3519
                     /locus_tag="ACHOWS_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0050378.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3550..4539)
                     /locus_tag="ACHOWS_004923"
     CDS             complement(3550..4539)
                     /locus_tag="ACHOWS_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0050379.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQSIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(4566..5417)
                     /locus_tag="ACHOWS_004924"
     CDS             complement(4566..5417)
                     /locus_tag="ACHOWS_004924"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0050380.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
BASE COUNT         1502 a         1216 c         1334 g         1595 t
ORIGIN      
        1 aaaaaaacaa gtttaaatga taagaaatgg aatataaaac tatcataagt cataattaaa
       61 gatgatttgc caatcagtaa ggtgttttaa tggattaaca tgccgcacaa aatggttgtg
      121 ggtgttcatc ccaataagtg atgaaaaatt aaacaggtgt aagtacgtca tctgtccggt
      181 aagcccccca agagccaaat taagtgaagt taaacgaaga ctgccagttg tcacaggaac
      241 gatggcctct ttgccagggc actgcattca gtcatctgta cgcgttactc taactggcac
      301 tcgaacgtcg gcgatggggt taccgatgga tctggccgtt cctgtggcgt aaacgttttt
      361 atgtcagtac caacagattt accacattta tcatcttaac agtttgagtg taaatgcagg
      421 ctgcgtcgca aagggaggaa actgagtggc gtgttcagtc taaaatagag ttgatgccag
      481 catacagggc gcagctggtc aacaagtcaa cattaatata atggaatatt cggagagaaa
      541 cgtcaggcag ttgaccttaa attaacagga agatacatcc ccaggaaaat taatgctggt
      601 gtaataaata caccaacatt aatccggtat gattattaat cagacaaata tcgttaattc
      661 gtcgaatttt gaatcaacgt tttttcttac gaccctgaac ggccttaaaa cgtggattag
      721 atttacaaat cacatacagc cgtccttttc gcttcacaat ctgacagtct ggatggcgtt
      781 cttttgcggt acgcagagag ttaaggactt tcatcatgcc ccctttttcg tgctaacaaa
      841 acgaccaaaa cgttgggtga accgtgcaac atttccttct gatgccactg ttctcagctt
      901 ccctgtatag aacgggtgcg atttcgaaga gacatcaatt gttacgtacg gatacgttac
      961 gccatccagc tcaatctcac ggtctgtttt gatagtcgag ccgattttaa agtactcatc
     1021 aacgctggtg tcgtggaaca ccacagtacg atactcagga tggatattgg gtttcatcat
     1081 ttttacctgt tatgttataa cataaccgta aagatacatg ctcatccgga tcatttcaac
     1141 acccaatcac tgtgactatt tgtgggatgt ggaatttccg caacggggaa gtctggcatt
     1201 gttagcagcc attggcgacg ctcccataaa cgcctggatg agtgattgat gatggtttcg
     1261 ctgagagtat ggatttttcg agaatggagg ctatctccac tttactggcc gatgatttat
     1321 tgcagcatga ttgattacct gacaatgtgt attcctctcc cacggatata ataattactt
     1381 acaaaaaagg gagaggatgc atattttaaa tatcactgaa gtgaacagtt tattgccgtt
     1441 attaatagaa atggagaaat aaataggcgt attctacaat tgcgacaaaa acaacgatat
     1501 taatcagttt atgactgatt tgctgtactt tattctcttt cattggtact tcctcgcttt
     1561 aaaaaagagt gcacttcgta agtgccctta tataaataac gagtttggtc aaccaatttt
     1621 ttgacatgta tcacaaattt gaatagatgt attacatcgg ctatctttta ttgccccaac
     1681 gtcattgata tatgtcgcct gaaatcagtt acgggaatga gtctgatctc aagactggcc
     1741 cagtcctggc gttgattggc gctgaggagc atattgcatc tcatcgtaac gtcgtatctc
     1801 ctggggtgtt atacaagata tcgttgtagg taaccaggga gaggaattga gtctattaaa
     1861 ccgtcaacta tgccggatac atactggatt acactgcagg cacgccttac gagagaacgt
     1921 gccgcagtga cgggttaatt atctgaaaga atttgcgagg ctgtatcggt tactcattga
     1981 tttgatagtt ttactctcgg gagagtaatc gatatttaat ccattaacgg aaaccagcca
     2041 gttcctttcg atgtctgaat ttccttggcg tgacataggt atatcctgta attactaaac
     2101 caatattttg tgagcgacgt ttataatatt cgagttcttg ctcagagatg gttccatcag
     2161 gattagctga ccaggtcgtc ataggagcca taacgatacg attacatggc tttatttttt
     2221 ctgtcagcat aaaaggagaa aacagtgata ggtgtttatt tgtcatgaca atacctcaaa
     2281 tgtttatctg attttgatgt gtatatttaa actggttagt tgttaccagc tttggaacca
     2341 agatttttgg ctttgttttt atttataatt actgttagtg cttttattga ttttatggtg
     2401 gagaaaagag taaaaataga aagttaatga tgtgtgtcag aatatcggta agaagaatgg
     2461 cacaatcgtg agggtacgtt tattcataat aaatctcctg aaagtttaga attattttcg
     2521 atacagccag attatcattg ttactaataa tgtggttaaa ataatagcat ctatgccccc
     2581 ttcatgagtg tgtgtgaaga ggatgcttct acaaggaaat cgccaatgct caaagaaaac
     2641 ttcaacgaac tgcaaatctt tcttgtggtg gcaagagagc gaagttttac caaagcagcg
     2701 ggcaaactcg gcgtctctca gtctgcactt agccatgcaa taaaggctct ggaggaaagg
     2761 ctcaatatcc gcctcctgac acggactacc cgcagtgtgg ctcccacaga agcgggggag
     2821 agaatcattg cctgccttga accgcgtatt gacgagcttg aacaggaact ggaatcactt
     2881 attcaattga acggcacgcc ctccgggaat attcggttat ctgccgggga acatgccgcg
     2941 cgaagtctgg tctggccgaa gctaaaacct tttctcagag agtatcccga aatcaatgtc
     3001 gaactggtgg tggataacgg ttttgtcgat attgttgaag gccgttttga tgccgggata
     3061 cgactgggtg aaaatgttga taaagatatg gttgcagtca ggattggacc agatatgcgg
     3121 atggctgtgg tgggagctcc ggcgtatttt gctgcaaacc ctgcgcctga aacgcctcac
     3181 gagctacaaa atcatcgctg catcaacatg cgtttgccaa ctgccggtgg gctttaccac
     3241 tgggagttcg agcgagaagg gaaaccgctt cgcgttaagg ttgatgggca actgacgtgt
     3301 agtccattgc cagaaagaat tgatgccgcg ttatcagggt ttgggatcgc ttgtgtccct
     3361 gaagacatgg ttcaggagta tatagagtca ggcaagctta ttcaggtgtt acaggagtgg
     3421 tgtccaactt ttcctgggta ttatctttac taccccagcc gcaagcagca tccgccagct
     3481 tttgcgctgt tgatcgatgc gcttcgctac accgaataac gggtccttta tcctggaccc
     3541 gtttgggtat tagcggccta cgcgtgcctg atgttcaggc gagtaacgtt caccgacgat
     3601 tttaattgtt tccagcgcct cgcttatctg ctgcgtatct ttctgcgaaa gaacaatgtc
     3661 agcagcggcc aggttttctt ccagccggtg cagtttagtg gtgccaggga tgggaacaat
     3721 ccacggcttt tgtgctaata gccatgccag tgcgatttgt gcagacgtta cccctttctc
     3781 cgcagccagt tcacctaaca aggtgaccag tttttcattg gcttcaatgg attgtgctgc
     3841 gaaacgcggt acggtgctgc ggtaatcatc tttgccgaaa gtggttcccg gcttaatcgc
     3901 ccctgtcagg aaacctttac ctagtgggct gaaggggaca aaaccaattc ccagttcctc
     3961 cagcaacggc agaatttcct gttcaggctc acgccaccac atggaatatt cgctttgcag
     4021 ggcggtgaca ggttgtaccg catgtgcacg acgaatggtt tgtgctcctg cttcagacag
     4081 gccgaaatgt ttgaccttac cttctgcgat gaggtctttc accgttcctg caacatcttc
     4141 aataggaaca tctggatcaa cacggtgttg atacagcagg tcaataacat ctgttttcag
     4201 acgacgtaat gatccttcaa cggcttcacg gatatgttcc ggacggctgt ttaaaatctg
     4261 ctgcttatta tcatcgccga aggtaaaacc gaatttagtg gcaattacca cgcggtcacg
     4321 aaacggtttt aaggcttcgc cgaccacttc ttcattcaga tacggaccat acacttcggc
     4381 ggtatcgaag aaggtgacgc cacgttcaac cgcagcacga ataagttcga tagcctgacg
     4441 ggtatcggtc gccgggccgt agccgtggct taagcccatg caaccgagcc caagggcgga
     4501 gacttcgagt ccggatttac ccagataacg tttttgcatt gaatgaatac ctcttttatc
     4561 gcggcttaaa catcaagttt gcggccagtc agccattcca ccattgcggg atcgcggtga
     4621 gagaagaacg cgctggttgc ggtatccagg gcggtaattt gcagcatatc ttcagggctg
     4681 agttcaaaat cgagaatgtt gatgttctct tccatgcgtt cttttcgcac tgattttgcc
     4741 agtgaaacaa tgcctcgttg gtagatccag cgcagaacaa cctggcccac gcttttgcca
     4801 tacttctcgc caattgccgt gagaacagga tgctggaaca gaccattttt cccttccgca
     4861 aacggtgccc aggcttctgg ctgaatgcca cgactttgca tccagggaac ggcctgtagc
     4921 tgctgattga aagggttaac ttcaacctgg tttaccgccg gaacgacgtt attaaaagcg
     4981 ataaggtctg ccagtcggtc aggatggaag ttactgacgc caatagcgcg aattttaccg
     5041 gcctgttgca gctcttccat ggcacgccag gctccgtgga catcgccata tggctggtga
     5101 atcaagtaca ggtcaacgta atcaagttgc agtcgattca gagagcgttc gaactgggct
     5161 ttggcccctt cgtaactggt atcctgcaac cagagtttcg tggtgacaaa gagttcgtta
     5221 cgggcgatac cgctttgttt cagtgcgttc ccgacctgga tttcattttg ataagaggcg
     5281 gcggtgtcaa tcagtcgata accgctgtta atagcgtcaa tgaccgctct ttcgcattcg
     5341 gcggcatcgg tcatctgaaa tacaccaaag cccaacaggg gcatttcaat accgttgttc
     5401 agttttactt tttgcatggt gttatccttc cgttatcgtt catgagaagc ataacgtaaa
     5461 gggaagagct cgattagacg gcaggatttg tcaggggtta tgaacaaaat tcataaatct
     5521 gtttgagggt tgagattagt cgtgcaagtt cgatatcccc ggaatttaca gtccgatgaa
     5581 taaaaatgaa tgtccataaa aaatatattg gtttatccca gcgtatatac ttacttcgac
     5641 gtactcc
//
LOCUS       DAZKJR010000230         5628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKJR010000230 DAZKJR010000000
VERSION     DAZKJR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.231"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1750
                     /locus_tag="ACHOWS_004925"
     CDS             <1..1750
                     /locus_tag="ACHOWS_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFQ0050381.1"
                     /translation="MGPKGEKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPR
                     GEPGPAGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLNSATDSDDETKA
                     ATPKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEF
                     VAWLKEKGAFDQPYWMMKASLHAEFNKVITDVGSGKLNLGGCAIEVMGTYNAAIVRVT
                     IGEYGGDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSS
                     DTDSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGDKGDTGPAGPAGPG
                     GVQGPKGDPGPQGPKGDTGAAGAKGEKGATGATGPQGPKGDTGAAGPAGPQGPKGDKG
                     DPGVAGPAGPAGAPGLKGDKGDPGEAGPAGPAGPAGPEGPQGPKGDKGDPGAPGQGTE
                     LLTTANTWTQAQTFNGGINGNLTVNGNGSFNDVQIRSDKRNKRNLVKLDNALDRLEAL
                     TGYLYEIQYSADGWQTSVGLIAQDAQKALPELVTEDADVISGEKRLRLNYNGIIALLV
                     EGFKTLRHEIKELREK"
     gene            1837..2349
                     /locus_tag="ACHOWS_004926"
     CDS             1837..2349
                     /locus_tag="ACHOWS_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050382.1"
                     /translation="MGITSGWVGSSAKSETGEQWMGAAGTKLGLDKPFMMSQMVGRAM
                     GCKIETAYYKWNSSDQVENWGAVGTDWPLEEKSKGTITNAENCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVTQDLKIS"
     gene            2583..4208
                     /locus_tag="ACHOWS_004927"
     CDS             2583..4208
                     /locus_tag="ACHOWS_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050383.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWPDVVDVIRSPVAQDAHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQSGDVSDDNPNDDETRFYTETFVSAYG
                     EEGPPGPESLEVTVGIPDTPVQLTLSPVPLQDANINRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKDLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPTSIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPANMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGDS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPEQVGVTIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            4205..5473
                     /locus_tag="ACHOWS_004928"
     CDS             4205..5473
                     /locus_tag="ACHOWS_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFQ0050384.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGAKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            5488..>5628
                     /locus_tag="ACHOWS_004929"
     CDS             5488..>5628
                     /locus_tag="ACHOWS_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050385.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQ"
BASE COUNT         1488 a         1190 c         1673 g         1277 t
ORIGIN      
        1 tatggggcca aaaggcgaga aaggtgatcc gggaggtcct gcaggcccgc aaggtcctaa
       61 aggtgaacga ggagaagccg gaccacaggg accgatggga gcacgaggtg agcgtgggga
      121 gactggcccc cgaggtgaac ctggtcctgc aggtccgaga ggcgaacgag gagagaccgg
      181 acctcaggga cctcgtggag agccaggtcc ggcaggcagc gctgcaaatg tggctgatgc
      241 gacgacggca cagaagggaa ttgtgcagtt aaacagcgca acggacagtg atgatgaaac
      301 gaaggcagcc accccgaaag cggtgaaagc ggcaatggac aaagcggacg gatgcctgga
      361 gaaagcgaaa aacggtgacg atatcccgga taaggtgaag tttctgaaca ccgtgggagc
      421 agccagagta tacgggcggg acattcatac gggggccggt gaatggacca cgagtgagtt
      481 tgtcgcctgg ctgaaagaaa agggggcatt tgaccagcct tactggatga tgaaggcatc
      541 actgcatgcg gaatttaata aggtcatcac ggatgtcgga tcgggaaaac tcaatctggg
      601 aggctgcgcc attgaggtga tggggacgta taacgcagcc atagtccggg tcaccatcgg
      661 cgaatacggt ggcgatggtt ttctgaacgg cacggtctgt acctgtacag tttacggaga
      721 cacacaacgt tttcactggc gggtggatta cagcacaaaa aacaaaccgg atacggtcag
      781 ccagcgggat gccagcacga cgcagaaagg tgtggtgcag ttaagcagtg atactgacag
      841 taatgacgaa acaaaggcag ccacgccgaa ggccgtgaag gcggcaatgg atgtggcaaa
      901 tgaagcgaaa acaaaggcag aagaggctgc agcaggaggt ggtgttcccg gtccgaaagg
      961 agataaaggg gacacggggc cagcaggtcc ggctgggccg gggggcgttc agggaccgaa
     1021 aggtgatccc ggcccccagg gaccaaaggg ggatactgga gctgctgggg caaagggtga
     1081 gaaaggagcc accggcgcaa ccggaccgca gggacctaaa ggggatacgg gagccgcagg
     1141 cccggcagga ccacagggac caaaaggtga caaaggcgat ccgggggtgg ctggaccagc
     1201 aggtccggca ggtgcgcctg ggctgaaagg cgataaaggt gatccgggag aagcaggtcc
     1261 agcaggtcca gcaggtccag caggtccgga agggccgcag ggtccgaagg gagataaagg
     1321 cgatcctgga gcccccgggc aaggaacaga actgcttact actgccaata catggactca
     1381 ggcacaaact tttaatggtg gtattaatgg aaatttgacg gtaaacggaa acggatcatt
     1441 taacgatgtt cagatccgct cggataaacg caacaagcga aatctggtaa aactggataa
     1501 tgcgttagat cgtctggagg cacttactgg ttatctttac gagatacagt actctgccga
     1561 cggttggcaa acgtcggttg gtttaattgc tcaggatgca caaaaagcct tgcctgaact
     1621 ggtaactgaa gacgcagacg ttatatctgg tgaaaaacgt ctgcgtctta actacaacgg
     1681 cataattgca ttgttagtcg aaggttttaa aacacttcgt catgagatta aagaactccg
     1741 ggagaagtaa acgacagctg ttgtagtttc tggtttctac tgaatttaaa ttgtggggat
     1801 gatactcacc ccacgaattt cagaaggata tatgaaatgg ggataacatc gggatgggta
     1861 ggttcttcgg ctaagagcga aacaggtgag caatggatgg gggctgctgg cactaaacta
     1921 ggattggata aaccttttat gatgagtcaa atggtggggc gagctatggg ttgtaaaata
     1981 gaaaccgctt actataaatg gaactcttcg gatcaagttg aaaactgggg ggcggttgga
     2041 actgattggc cattagaaga aaaaagcaaa gggacaatta caaacgctga aaactgtggt
     2101 tctgggagac tggtgggggc tgtcgttaca ctttctcact ttttgacgaa ctctacaccg
     2161 acagctgctg tttatttagc cggtggtaaa gcaggtaaca tcaccgtaaa cgtaggtggt
     2221 gctacacaaa ccatgattta tcagggcgtt gttagtgggt tccagtatta ctggtcaggt
     2281 tctgttagtt ccgctttcgt ggaggcaatg aaaaagacgg gagtaaccca ggatctaaaa
     2341 attagttaaa tggtaaatga ataattttaa aaacttcacg ttctacatac cggagacgcc
     2401 ggatatatag gatattgttt taagttgcca gagaaatttt ttccggacgg atgctgataa
     2461 taatgatgta atttttcaga tttctggaaa tctgtattct gcacaggcgc aattgtatgc
     2521 cgcctttaaa acttcaattc agtgactcac ctgccattca aattttcgga taccagacaa
     2581 ccatgcctta tatcgatata acaactatgc gcgggatgat gccaggcgtt attgcatcta
     2641 tgctgccaga tcattctgct gtactggcag aaaactgtca ttttcgctat ggagtaatca
     2701 cgcctgaaca ccagatgtca gaggctgaga aaacattcgc gattaagccg aaaaccattt
     2761 ttcattaccg tgacgatttc tggtttgcat ggccggatgt ggtggatgtg atccgcagtc
     2821 cggtcgctca ggacgcccac gggcgtattt actacactga cgggcgtttt cctaaagtga
     2881 cggatgcgac cattgccaca aaaggggacg ggaatcaccc gacatcatcg tatcgtctgg
     2941 ggatccccgc gccgacgaca gctcctgtct gtactgttca gcagagcggt gatgtttctg
     3001 acgataaccc gaatgatgat gaaacccggt tttatacgga aacctttgta tcggcgtatg
     3061 gtgaggaagg gcctcccgga cctgaatcgc tggaagtcac cgtggggatc cctgatactc
     3121 cggttcagtt aacactctct ccggttccgt tgcaggatgc aaacatcaat cgtcgtcgta
     3181 tttatcgttc tgcctcaggg ggaggagaag ccgatttttt actcgtggct gaactggatg
     3241 catccgtgct cagttacacg gacaaaatac cggcgaaaga ccttgggcct tccctggcga
     3301 catgggatta cctgccgccg ccggagaata tgacaggcct ttgcctgatg gctaatggta
     3361 ttgccgccgg gtttgccggt aatgaagtga tgttttcgga agcgtatctg ccgtatgcat
     3421 ggccggaagt gaatcgtcac acgacggcag aagatattgt agctatctgt ccgctgggaa
     3481 cgtcactggt ggtggcgaca aagggggagc cttatctgtt cagtggtgta tcgccttcca
     3541 ccatttctgg ctccagaatt ccgtccatgc aggcatgcct gagccgaaga agtatggtgg
     3601 cgatggaggg attcgtactc tatgccggga caaacggtct ggtatctgtt gatgtaaacg
     3661 gtaatacagc actggcaacg gaaaagatta tttcgcctga acaatggcag agtcagttta
     3721 acccgacgtc cattgtggct tattcctggc gtggtgagta cattgcctgt tacacgaaac
     3781 cggatggtaa gcaggatgtg tttgtcttca gtccggcgaa catggatatc cgttatctca
     3841 gtacaccgtt tgactgcgca tgggttgatc tcgcgaaaga tatgatgcgc gtagtgacag
     3901 gagacaaaat gtcagtgctt gccggggact ctctgccgtc cacgataagg tggcattcaa
     3961 aaattttttc attacctgaa agaacctctt tttcctgtat cagggtgaaa tctccggcgc
     4021 ctgagcaggt gggggtcacc attatggctg atgatgttcc tgtgattcat tttgcgccgg
     4081 gtacgtttaa gggaagtgtg gtgagacttc cggcagcaac cgggcaaaac tggcaggtga
     4141 tggtatccgg attcgggcag gtggaacgaa taaccctgag tacatcaatg tcggagatgc
     4201 cggtatgacc agaaaaccgt ggcgtgcggg gaaggattta tccactgttg tggagaatat
     4261 ggaaattggc accgggcagc gtggtgacgg acgtcacgca tttgtgaccc gtgaggaact
     4321 ggttggtctt aaactcgccc ggcgtcgaac atcgggtggt gcctcatatg cactgaatcc
     4381 gggtattgag attgacagta ctttaatgac tgttgatttt cccacaaaac cgctgaattt
     4441 taaggcgacc ggtggatttg gctcggttct tcttgaatgg gatatgccta attatcgcgg
     4501 acattcactg actgaaatct ggcggggtac ggaggatgac cttgctgatg cagtgctggt
     4561 tgccacgacg ccggggcagg tttacggcga tccggttgac cctggctggt cgggatttta
     4621 ctggatacgt tttgttaacg cggcaggagc taaaggtcca tggaatgctg aaaaaggcac
     4681 tcaggcacaa acacagatcg gcgtgaaggc tatcattgac cagatccgcg atgaggctgc
     4741 aaagtcgccg gttgtgtccg agctgcgtaa agaaataaaa aacgcgcagg ggcaggctgt
     4801 aaaggatgct gcaattaaga caaccgaagt tgtagggact ctcagggaag aaacgacaag
     4861 aacgattggt ggtattgaaa cccgcattag cacactggat tcgtcaacca gtgaatcgct
     4921 taatgaggtc gacaagcgca tcactaaact ggataaagaa ggcggtgagg cgtttctggc
     4981 aatgtggtca aaaaaagcgg gagttgatgg tatcactgcg gggatcggga ttgtcgccgg
     5041 aaaagacagt gaaggcaggc ctgtaagtca ggttgcaatt tctgcgtcgc agttgtttgt
     5101 ctttgacccg aacaacccgg ataacaccgc ctatccgttt gcggtatcag gtggcaaggt
     5161 tgtgatcccg aaagcgatga tttatgacgc ggtgattgaa acactggtgt cgcggaaggt
     5221 tgtggcggat gaggtaaaag ccggggtaag tatcacttcg ccagttatcc ggagtgccgt
     5281 tattcagaac ggaaactttc aggttgattc tcagggtaac ctgaatattg gaggcctttt
     5341 cagtgttacg tcacaagggc aactgacaat tcgttactct aatcagaatg taggactggt
     5401 gatccgcaat gataaaattg aggtttatga tcagaatgga cgactggctg ttcgcatagg
     5461 cagattacgc tgatcaggag gtgagtattg gaatacggtt ttgccattta taacagaaat
     5521 aacgttaatg ttacgggcgt gctgactccg gtatttttcc tggacagatt tacagcggag
     5581 tctggctcaa agacgtacac gaataaaccc gacgggaaat cattgcag
//
LOCUS       DAZKJR010000231         5582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKJR010000231 DAZKJR010000000
VERSION     DAZKJR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.232"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            195..451
                     /locus_tag="ACHOWS_004930"
                     /pseudo
     CDS             195..451
                     /locus_tag="ACHOWS_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(473..744)
                     /locus_tag="ACHOWS_004931"
                     /pseudo
     CDS             complement(473..744)
                     /locus_tag="ACHOWS_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(741..2501)
                     /locus_tag="ACHOWS_004932"
     CDS             complement(741..2501)
                     /locus_tag="ACHOWS_004932"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFQ0050386.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPRITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPSADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSSVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            complement(2494..3135)
                     /locus_tag="ACHOWS_004933"
     CDS             complement(2494..3135)
                     /locus_tag="ACHOWS_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0050387.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            complement(3139..4056)
                     /locus_tag="ACHOWS_004934"
     CDS             complement(3139..4056)
                     /locus_tag="ACHOWS_004934"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0050388.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPSKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            complement(4053..4658)
                     /locus_tag="ACHOWS_004935"
     CDS             complement(4053..4658)
                     /locus_tag="ACHOWS_004935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0050389.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            complement(4716..4901)
                     /locus_tag="ACHOWS_004936"
     CDS             complement(4716..4901)
                     /locus_tag="ACHOWS_004936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050390.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            complement(5142..>5582)
                     /locus_tag="ACHOWS_004937"
     CDS             complement(5142..>5582)
                     /locus_tag="ACHOWS_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFQ0050391.1"
                     /translation="DRIDNITGYILIVAGVLSASFYVVISSRLIVNFEPVTLLTGQLT
                     FSFILACFFIVLTGTSLQLPIESVFLVFLSGILQYFLAFCFYLYSLRWIPVHIAGAML
                     YFIPLVALLLSWFFLQEDISFIQGLGVFITILCVYILNKKYAIE"
BASE COUNT         1536 a         1475 c         1353 g         1218 t
ORIGIN      
        1 ataatcggcc tctaaaaaat atattgtatc cactttgttg tggttctgac agaagtaaat
       61 taatttgaag taaataaagc aaaatatcag aagaatatat cggggcgcac agtctatctc
      121 tgcctttgct tcatgggatg ttctttgtat attttacttg ctgtttaagt atgtatataa
      181 ggagtgcatt cattatgcca atgaagcttg taaagaagaa tccccggagc aaagtgattc
      241 gggagcttag ctgtgagcaa gagatggcag taaaggcggt ctgcggactg aaaaaacagc
      301 aactacggca ctacatgcct tggccaatga tcttctccgg gaaatgcgtg agtatgatgt
      361 atggttacag tgtgactgta ttccgggaga ttcgccgacc atgaattttg ctgctcttaa
      421 aaacaacacg ggaacttttt atctgtccag tttggatata gataattatt gtactaataa
      481 gggaagccaa caccagccga cagaccagac gccgctgctc attcagtgaa acgcagataa
      541 acagtaaagc aaatccagac tccagtacgg aataatcgtc agcggtatta acgggatccg
      601 gtgctcccag tcaaacacca ggctgctgat gttttctctc ccggcggtaa actgattcgt
      661 catgccataa gtaagaaaga agaatggggc cagcaccatt agccagccga ttccctgaat
      721 aatcaccgca cgccgcgttg tcatggttga cgtcgtaccg ccatagagac cgtgaagatg
      781 cccatttcat caattagttg cgtgcatttc tcaaaaccgg cgtcacggac cagcgcgtcc
      841 atctcgccct gagtacgtac gcgcattacc caggctttgc catttttgtg actggtgagt
      901 gaccaggcga tggtttttaa ctgagggtgc cagggctgac aggtgtagat cagaatgcct
      961 cccggttcaa tcgctgcagc cagcccggca agcgaatctc gtaccggccc gttttccggg
     1021 aacagttcat acagaccgga cacgatcccc agcgtcggac gcggttccag tgccgccagt
     1081 tcattacgat taaaggcatc cccctgctca aatcgcgcac ggcccgccat accgcgagag
     1141 gctatcatct cctgaccttt cgccacgttt aattcgctgt aatcgcgcag aaggatctcg
     1201 cttaccgccg tttcgccttg cagggcatca agcacatagc gtccatgccc ggcggcaata
     1261 tccacgatcc ggaccgagct accctgctct gttaaccgag caacagcctg ctgaattaac
     1321 atctgcagat gaattttacg ctggcggatc cccttccagc ccacgctatt caggtagttt
     1381 ttatcaatga ggcgtcccaa cgaggtgctg ccctgaggct ggttgcgata gatgtagtct
     1441 agggtactgc cggaatcgaa accagtctca aatcccagac gcacccccgt ggactgcgtg
     1501 cccagcactt tcatcccgta gcgcaaacag cggtaagaga tatcgtccag cgaatacagc
     1561 gcagggccac cgctcagtat gcgccaggta tcggcgctgg ggctccagcg atcttcctgg
     1621 ctgtaatcaa aacgctgcac gggacgggca tacaaggtat caataaaact gcgcatcctg
     1681 tcaaatgcca gatgccgctc tttttcgcct aaggtgtcat gataaaatcc cggcagtaca
     1741 tgcagttctt tctgcggatt tcgcaggttc tgataaaaat caagctgcgg ctggcggtgc
     1801 acaacgtaat cttccccgga gaccagcaac tgcacaggta gcgtaatggc gctggcatcg
     1861 ctgacgactc gctcagccgt tttgtagaga tcaagcagaa tattcacagc aattggtcgg
     1921 gtaatccgcg gatccttatt gaagcttgct acccgctcgg gatcgtgcgt cagatacttg
     1981 cctttcacat acgagttgac gaagaaaaga ccgcgcaggc gatacatcaa ttccagcccc
     2041 tggcgagcaa acggcacata caatttgacc ttaaacgccg gtgatgccaa caccaggcca
     2101 cgaatgtccg gggcataatc atgcgcccag gttgccgcca gaaccgcccc gacgctttgc
     2161 gccacgacga ccacatccgt catcccagct tggctatctg cggcggcgaa acgcacaaac
     2221 tcgtcaacat cctgcaccga acgcacgagc gacggactgt aaccacgcag acccggtgag
     2281 cgaccatgac ctcgggcatc catggcataa aacgctgtat ccggcatcgc cagctcatca
     2341 acaatgtgtt gcaaacgccc ggagtgctca tgcccacgat gaaacaggac caccactttt
     2401 ggcgttgaac tggcttcttg tgctggccag tggcgataaa acagctctgt attatcactg
     2461 gtcagaaact gccgttcatc gggtatgcgg gaattagtca tggggatgat ttccttgcga
     2521 gggttgcaaa agcgccgtta gtctttgctc caggtcgtcc ataaacgttt tcttctccgg
     2581 atgcgtactt agcgcatcgc caatatttac atcaataaac agcggcaaag ggatacgatt
     2641 acctttcgcc atcacacgct cagtaccgcg caacccaacg gggataatgg gcacctccgg
     2701 catgcgctga ctcaaatgcc acactccgga tttcaatggc gcaagtacgc ctggctcacc
     2761 gcgcgtccct tccggaaaca ggatcagtgt tttaccttca cgcagcgcct gttcacattg
     2821 cgccagcgga tcggtataac cgccctttct tgaaatagga atgatgttaa ggatcttcag
     2881 cgcaaaccaa gccatgcgtt tatcccgcag aaaataatcc gcggcagcaa ccggatgaac
     2941 agaagcctgg taacgcagcg gaaaaagcga aagcaaggcg aaaacatcca tatggctgtt
     3001 gtgatttgcc acgattatcg aaggcccgtg cttcggcagt cgctcgcggt ggcggacacg
     3061 cagaccaagc cacagccaga tgaccggcca gacgatacac agcgtaaaca gggtacggag
     3121 tagtcgtgtc atcataggtt agtagcagaa atagcgaaca aaatggaaga agaccggtgc
     3181 ggtaaaaatc agcgaatcca gccgatcaag gatgcctcca tggccgggta gcagcgtacc
     3241 actgtctttg acgccgatat cacgcttgat tgccgacatc accacgtcgc cgcaaaagcc
     3301 agtaacgccg ataataaccc cggctaacag tgccatcggc cagctcaggg gagtcaacag
     3361 tggccccagg actgttgccg ccagcgccgt cgttgccacc ccacccagca atccctccag
     3421 ggttttgttt gggctgacct tcggtatgac ttttatacga ccaagcgact tgccccacag
     3481 gtattgcgcg atgtcgttaa attcggtcag gccgaccaaa aacagcacca acagcgcgct
     3541 ggtttgcatt ccatccgccg gtaacaccag taaaaacgcg acatggctga aggcaaatac
     3601 ggtggtcatc agactccagt gcaactggga aacggtacgt aaaaagccct gtgtatcccc
     3661 agcaatcacc attcgcgccg gtaaaaaaag gaacacgtag accgggataa acaccacaaa
     3721 catgccgtac cagccgatac ctatcagcca gtagttaagg ggaattgcaa taaacatcca
     3781 taacagcggc atgcggtcgg actggcggga aggcaccagc gtcaaaaact cttttagcgc
     3841 cataaagctg accagcgcga agaacgtcag tgccagccag ctaggactta atagtgcgaa
     3901 ggaaaacagg acgataatca cccaccaggt gcgaatacgt aatgtcagct cgcgccagtc
     3961 tttacttgga cgcagccaca ccagcaaccc gttgattacg gtggcaatca acagcaggga
     4021 aaaaataacg atcagtgatt tttcaagtag cgtcatttca caccctcttc acgcaatgcg
     4081 ctgcgacagc gattaaaaat cgtccataac aacaacagtg ttgcgacacc ccatatgaca
     4141 ttattccagg cgacaatctg cggccagatc gacagtgaca gcccccatgc accaaacacc
     4201 agcgctctgt cgcttttgcc cagcggaccg gcatagctgc gcacgccgtt aatcgtttgc
     4261 gccagcacgc cgcaaaactc ggtcatcagc gcacaaaaca ccacgatcag caccagcggt
     4321 atactgctct gcgggagtag cataaagggc agataaagcg agatgtcgga aattacatca
     4381 cccgtctcat tgaggatggc ccccagacgc gatctttgat tacattcacg cgccagcata
     4441 ccatcgagcg cattgagcgc cattcgtaca aacaacacca cgggcagaag gacgaataat
     4501 ccgggctgag gatgaataat gagcaccacg ccggtgataa gggaaagcac catcgcgcac
     4561 agtgtaatgt gattagcggt gacgccgcgc ctgttcagcc agaacatcag cggtcttaac
     4621 agagcctgaa acgagggctt tatctggtaa agggtcatca tcactcctta ataagcatat
     4681 ttgacggggt tgagtctggc cttttattgg ggctgtcact ggcacactga aagtgaatac
     4741 agttccgggc gcggaaatcc tgcctgctgc gccggtaaag gcgactgact ggtattacgg
     4801 gaggccagag agccagtacc tgtgccggta gactgtgtgg ctgaaccata aacatagtgt
     4861 cctggacaac ggtacagcac tctgtgcagc tgtgtcagca tgcgcttttc tttaattatg
     4921 cccagtcatg tgcggaggca aggatttcaa aatatccgtt acatttgttg catgatgcct
     4981 tatcgctggt ccgggctatt gctttacttc ttttaaatca gctctgatga acatttttta
     5041 ccactttatc gcattaaggt tatgtcattc atcgtctggc atcacgtttt tggttatggt
     5101 gtcagggtgt accctaccgc gataacgcaa ccctaataaa gtcattcgat ggcatatttc
     5161 ttattgagaa tatatacgca taaaatagtt ataaaaacgc ctagtccctg aataaatgat
     5221 atatcttcct gcaggaagaa ccaggacaac agcaatgcta ccagaggaat aaaatagagc
     5281 attgctcctg ctatgtgaac gggaatccag cgtagggaat aaagatagaa acaaaaagcc
     5341 aaaaaatatt gtaaaattcc tgacaaaaac acaagaaata cagattctat tggcagttgc
     5401 agggatgtgc cagttagaac aataaaaaaa caagcaagaa taaaagagaa agtcaactgc
     5461 ccggttaaca acgtaacagg ctcaaaattc acaataagtc gtgatgagat aacaacataa
     5521 aaggatgctg agagtacacc tgcaacaata agtatatatc ctgtaatatt atcaatgcgg
     5581 tc
//
LOCUS       DAZKJR010000232         5506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKJR010000232 DAZKJR010000000
VERSION     DAZKJR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.233"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1285)
                     /locus_tag="ACHOWS_004938"
     CDS             complement(<1..1285)
                     /locus_tag="ACHOWS_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0050392.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWSTYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLIMRFYLINN"
     gene            1572..2324
                     /locus_tag="ACHOWS_004939"
     CDS             1572..2324
                     /locus_tag="ACHOWS_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0050393.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVSDVIRKNLDLDIHVQTVFCIKRVRYMGSTPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            2338..3414
                     /locus_tag="ACHOWS_004940"
     CDS             2338..3414
                     /locus_tag="ACHOWS_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0050394.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWSGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            3414..4412
                     /locus_tag="ACHOWS_004941"
     CDS             3414..4412
                     /locus_tag="ACHOWS_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0050395.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            4417..5331
                     /locus_tag="ACHOWS_004942"
     CDS             4417..5331
                     /locus_tag="ACHOWS_004942"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFQ0050396.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
BASE COUNT         1683 a         1188 c         1190 g         1445 t
ORIGIN      
        1 cattattaat cagataaaat ctcatgatga gtccggtaat caggttaagt atcgcgggaa
       61 tgatcgtcat taacaggcag atcccccaca acgcctgtgg tgtttgttct acgccaggtt
      121 gatagtccat aaacgctaaa gcgattccga taaaccctcc cgccagtccc ataccaaact
      181 tttgaaaaaa gagaatcccg ccaaatgcca gtccagaaac acgttgcccc gttttcattt
      241 caccataatc cactgactca gcaatggatg cccaataaat gggaagctga atatcagtca
      301 gaaaggtcac cacaatataa aatgtaaaag ctaacactat agaatttggt tgcacgaaat
      361 acatcgcacc acccaccacg aacgccagta attgggtata acgaaatatt tttattttgt
      421 cgtagagtcg agtaagccag gtgcaagcga tcatagccag aacagaggcg atcacgcctg
      481 aagttaaaaa cggtgaaatt aaagcatccc caccattcag gtaatatttt gcataatatg
      541 cagcgacaga gttacgaata atcccgccaa acataatgat cgcaattaca gcgcccaaaa
      601 tgagccattg atcatttttg atcagtaacc gaaactgcgc ggccataccg agactgggtt
      661 ttggatggta gatgcgctct ctcacgctaa aaaaacaata caggcataaa cacgttgcca
      721 gaattcccat tgcccccatt gctatttgat aacccagttg tacgttattc ttcccaagat
      781 acattgcaga taaaggaaca ataattgtta cggcgaacat ggcaattttt gtcataacaa
      841 aacgataacc attggcgctc aatcgctctt gtggatcatc cgtgataacg ctgattaatg
      901 aaacataagg aatagcgatt aacgtatagg tcaaggtaag taacacatag gtagaccagg
      961 cccataaaag cttaactgaa taatcggtat taggtgtggt gaacatcaac caaatgctta
     1021 tcccaaacgg tattgacata aaaagcaacc acggccgata ttttccccaa cgtgtattcg
     1081 ttctgtctgt cattgttccc acaactggat caatcacagc atcaaggata cgtacagaaa
     1141 ataacagaat acctaagtcg acgggtttca gaccaaatac atccgtgtaa aaataagtga
     1201 tgatcatggc caaagacatc attactatcg cgatagccat atcaccagca ccaaaaccta
     1261 tcttttcatt tattgtaagt ttcatacaca gcactcattg attacgtgaa cttatattaa
     1321 aaacaaatat atctgaactc attcagcaca taactttacg agtgagcata aatgcattgg
     1381 ttgataccac aacaaaccat tgagtcaata ttgagcaaca tcacataaaa accaatttca
     1441 cttaatctca tgatttatat atttaattgt ggttttttct gttttatgtc acagatagtg
     1501 aacaagcacc atgcgtaata aaagttaaag aatgtagagt tggtatcaac cacttgaaat
     1561 gaggctttgt catgataaaa atttataaag ccagtaaagg tcagctaaag catcgtgaaa
     1621 tctttgatga aataaagaaa aaagtagata acggtgaatg gaaagaaggc aaagccatac
     1681 cgacagaaag ggaattgtca gaatattatt taagtagcag aacaactatt cgaaaggcaa
     1741 tagaaagtct taaacaacgc ggctacctgc atagtgtcca cgggcaagga acatttgtat
     1801 tgccttctcg tagtagagaa aatcatttat tgcatagttt taccgacgat attaaagctc
     1861 gaggtggtat accagcacag aatattcttg agataggcta tgttcctgtc agtgatgtta
     1921 tcagaaaaaa tctcgacctg gatatccatg tccaaaccgt attctgtata aaacgcgttc
     1981 gctatatggg aagtaccccc ctgggtattc agacatcatg gcttgcatta aatgacaggc
     2041 aagaaataac ccaagaagat ctgctggcga cgggttcgct ctatatttta ctggaagaaa
     2101 aactcgctat taaaccttta gaagcaacag aaattatctc tgcccggctt ccctcgcccc
     2161 ttgagcgtca attactggaa ctgactgccg atgatgtcgt attaagttgt acccgcgtca
     2221 cgttatccgt ggaacgcaaa ccaatggagt atgtagagat ggtgtatccc gcaagccggt
     2281 actcctacaa agcacgaatt acaaaagaca gttttagcgt gtaacagaga gaatattgtg
     2341 aacaaaatag aacaagctgt aatttataac aaaatattag gttctttagc ctgcgcaggt
     2401 atgggcgatg cattaggcgc agcgactgaa ctctacagca ttgatgaaat aaaagctcaa
     2461 tggggaggtt ttttaaatgc ctttgtttct cctcctgccg atacattcgc tggctcatta
     2521 aatggtattg cgggacttat caccgacgat tccagccaaa tgtatgtgtt ttcagaaggg
     2581 ttaattgagg ctggatttga taacttcacc aataatgact ggttagcttg tcttctgcgt
     2641 tgggccgata tgcagcccta cgcaaattat aaaggcccca caacagaaca aatcgtcaag
     2701 gcactaaaag aaggtagacc aacaaacacc attgggcgta ttggaacatc ttctcgtcag
     2761 gcacccaatg tcggcaccac gaatggagca ggaatgcgtg tagcccctgc aggattgatc
     2821 tggtcaggca agaaagaaaa agcgtgtcac ctggcgctaa taacttgcct accttcgcat
     2881 gacaccaata ttgcgattgc cagcgcttgc gccatcgctg cggcgacatc acaagctatg
     2941 ttaccagaag cttcgctcac atcattactg gacgccgcaa tctggggagc gaattacggc
     3001 gaacgcctgg ccaaacaata tgcacgatgt gtcgcagggc catctatcgc catgcgcatc
     3061 caacttgcag cggacatcgc cagacgagcc aatgacctgg agtcttgtct gcgagaaatg
     3121 gaaggtttgg ttggtaactc tgttgccgcc catgaatcca ttcctgcggc aatcggtcta
     3181 ttgctgtact gcaaaggaga accctgggaa accattcatg cctgcgccaa tattggcaat
     3241 gacaccgata gcatcgctac gatggcaggc gcaattgcag gggcatggcg cggttttgat
     3301 gcgctccctg aagataaata tgcctttttc cgcgccgtaa ataataaaga tttcgatatt
     3361 gaagcgatcg catcgggtct gacattgctt gcattagagg cacaggagaa ataatgaaca
     3421 tgccagcccg tactcctgtt atgccattag cgcaaactcg tgaggagata aatgctacgg
     3481 cggcatcgtt acgccatttg gcagctatct ggggtgaatg tttttctact tttcagctcg
     3541 ataaacgtgc tgttgaacgc gtctgtatag tgggatcggg cgactcctgg acggtggcac
     3601 tatgtgccgc cgcctggctg gggaaataca cacatctgtt ttgttacgca ttgcaaacgt
     3661 gggattttct tcagactgat ttaacccgtt atcagaaaga aacgttaatc attattctca
     3721 gtgcaagtgg cagaccatca atgaccgttg acgccctctg ccacgcagta tgctcaaacg
     3781 cgcaagtgct gggtgtgacg aattgccctg gaacaccatt ttgcgcaata acagaaaaca
     3841 tgctctatac atgggcgaat aagcagggta ttccaacgca aagtagtagc gtcacacttt
     3901 atgccctcct gcgcttagcg caaaaattat gtccaggctt aacaccactg cagattgaag
     3961 atgatttgga agggaaattt agccagatta atcagcactg gcagcaaaag aagcgccatt
     4021 tttaccaaca aaaagagata acgtttcttg ggagtggctt aagttgggga ctggcaatat
     4081 cggggagcaa tttactttct tgcggcccac agattcgtgc gacagccctt ccactggaag
     4141 aattttatca ttcattacgc ttacatcagg ctggtgtcgg tcagcattat attttactac
     4201 cagccacatc ctgcgagtcc ccattttacc ttgcaaccca aaaagcaatt gttgaacagg
     4261 gagcgacagc agaattaatc agttttatac ctgacgccag tgagtccaac aatttatttc
     4321 tggtcatgca gtggttatat gaaatgtgct ggcatttaag ttgtgattat gtcgacgcag
     4381 gcgggcaacg cgttagccat agggagaaat aagactatga agaaatttga tgtcgccgct
     4441 ttaggcagtg gaaatataga tattttttta tctatcccgt cactccccac tcgtggcggt
     4501 aaagttgtcg ggacacgtct gggtgaacaa gctggcggca cagtcgccaa cagtgcctgt
     4561 gcgatgggac aactgggtct caatgtggta tctgtttcat gtgtgggcaa tgatcattca
     4621 gcctcgatca ttcttgatgg atttaaaaaa tatcatgtaa attgtgattt cgttcaggta
     4681 attccggaat tgattgccaa taccgcgatt atctttattg atgaattagg tgaaaaaacg
     4741 ttggtttact cgcctggtag tgatcatgag tgggataaag aaaaagccct gcaggcaatt
     4801 gcgcaaagcc gttattttta tacgatgcca gcagatatcg agaaatttcg tatgctggcc
     4861 gaatattccc gtagccagat gacaaaagtt gtcgttgata ttgagcctca tatcattgca
     4921 acacctgaac accttgccag aattttacac cttgctgata ttgcgatatt taattatgac
     4981 ggttttattc gtggctatgc aacagagcca gattttacac ttctgcatga tatacaagac
     5041 gagtatcaac ttgacgctgt cgtggttacg ctggatgtta ggggagtaat tgcagtcaaa
     5101 ggcaatgagc aagtaaaaat aggtagttat acaattcccg ttattgatac cacaggcgct
     5161 ggagatactt ttaatggtgc atttgtttat agcttaataa aaaatatgcc cttaattgaa
     5221 gcactaaaat ttgcttctgc cacagcagca ataaatatta ctgcattagg tgccagaggt
     5281 catctgccga caccatcgga ggttaatctt tttctttcac aacacgacta aaatcatata
     5341 tatacaaagg taaatatcat gaaaataaga tatgccgcta tttttctggc attaccgctg
     5401 ttatgcacaa cagcgcaggc aaacagtttt cttcattatg ctggggagtt taacttccgg
     5461 acgggagaaa agcagaacaa tgtcacaatc gcggggctat cagcca
//
LOCUS       DAZKJR010000233         5428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKJR010000233 DAZKJR010000000
VERSION     DAZKJR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.234"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            264..1427
                     /locus_tag="ACHOWS_004943"
     CDS             264..1427
                     /locus_tag="ACHOWS_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFQ0050397.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ESELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1432..3579
                     /locus_tag="ACHOWS_004944"
     CDS             1432..3579
                     /locus_tag="ACHOWS_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFQ0050398.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3639..4886
                     /locus_tag="ACHOWS_004945"
     CDS             3639..4886
                     /locus_tag="ACHOWS_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFQ0050399.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            5172..>5428
                     /locus_tag="ACHOWS_004946"
     CDS             5172..>5428
                     /locus_tag="ACHOWS_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050400.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAV"
BASE COUNT         1358 a         1164 c         1546 g         1360 t
ORIGIN      
        1 ttgaagtatc tcacattttt attatcttct gtcagcgcgg aagataatgg cggtagcttg
       61 gatcaattcc ggtgtctctg catttcaatt ttactcaata ttttacccgg ttgcatcatg
      121 atgcctgatt atatttcccg cgtgtcccgg aggttcgggt gaagagaata ttatctttct
      181 gttttctttt ctctgtccac aacgtgttcg cttctccact gccggccctc ctgagggatg
      241 ctctcattca tgatccatca gtgatggagg caagggcaga agccacctct gcccgttccc
      301 gtgtggagca ggctcgttca gcacactggc cggtcgtcac agcaacaggc agtaaactcc
      361 tttcccagag tcaccgctac gactatgact acgactctga agatattttg cccggtatcc
      421 gcggtgaaat gaatctgttt gcttcgggag ctattgaggc ggatgtcagc cggagtgagt
      481 cggaagccga gtattaccgt tataaagttg atgaggctgg cgaggaaaca attcgctcct
      541 ttgtttcatt atatcttgat gcgctaaggg aaaagcaatc cattgcggta cttgaacaga
      601 gtctctcccg gcataacgca attcttaatg atcttaacac catcagtatt cacgataccg
      661 ggcgggaatc tgaatttgtt caggccgaag cccgcaggct gatggtccgg cagcagataa
      721 atacccgcac ccgagtgctc aaaaccacgc tgggaaaact ttcgacctgg accacaaatc
      781 cggtaaccga agccgatctt gaaaatcctt tttcccggat gacagagtcc agattgttaa
      841 ctgattttac gcagacgcct cagaaaggga atccgtcgtg gcttgccagt caggctgatg
      901 tagagagtaa aaaagcggcg ctgaaagcgc aggagcttgc ccggtatccc cgggtggatt
      961 taacgggatc agtgacccgg gatgacaggc agataggtgt cagtctgtcc tgggacctgt
     1021 tcaaccgtaa tgccagttat ggtgtcagtg aaaaggccgc gcagatagct gcagctaccg
     1081 gacgactgga ctctgttgcc cgaatgattg atgaaaccgg acgattatcg ctgataacgg
     1141 tcagacaaag tcggatggaa atggaaacgc tgagacgtca ggagcaggcc tctgccagag
     1201 ttgtggactt ttatcgtctt cagttcaggg tggcaagaaa gacactgatt gaattactga
     1261 atgcagaaag cgaactgtac agtgtcgggc tttcccgggt tcagacggag gatcagatgc
     1321 tccacggaat gctggattat ctgtattccc agggaatgct cctgaaatgg agcggagtga
     1381 atctttctgg tgcagaagaa aaagtaacag ggcagaagga cagataaata agtgaagagt
     1441 atttttgaag gattatcaca ggtaacggca ttactgggta cacctgtatc tgctgagacg
     1501 ctggctgccg gtacagtccg gacagatgtc tccgggattg attttcgttc tgttggcgag
     1561 tttcttcgca gtgaagggtt tgataatcat ctttcccggc gggctccgga agatattcct
     1621 tcacttgcgg ttcctgtgct tcttttactg aatgcacagg aagccattgt ggtggtgcgt
     1681 attgagggtg ccggcctggc ccggcagtat catgtactcc agcccggggg actccgtaag
     1741 acactttctc acgatgaatt aatggctgtt tattcaggtt actgctggtt tgttcagcca
     1801 caggcaacac aggatcgccg ttcggaactg ccggagtatg acatgaccgg ggcatggttc
     1861 tggcgggtac tgtggcggtt caggccatat tacggacagg tcattcttgc gagttttatc
     1921 attaactttc ttgccctggt cagttccctg tatgtcatga acgtttatga ccgggtcata
     1981 cccaatcgct cctgggaaac gttatggatg ctgagtatcg gggtgattgc agccattctg
     2041 tttgagtgtg ctgcaaagtt aatccggagc tatctgacgg atattgcggg taagaaggca
     2101 gacctgatta tcagctcagc actgttaagg cgggtgatgg cagtgaaaat gtcggacagg
     2161 ccggcgtctt ccggttcata cgccagcaat ttgcgggaat atgagtctgt cagggagttt
     2221 atgaccagcg ccacgctgct ggcttttgtg gatttgccgt ttatcctgct tttcatcggt
     2281 gtcatcgggc ttgtgggggg ctggcttgct gttgttcctc ttacactggt tccgctggtg
     2341 attctggcag gaatattact gcagcgaccg ctttcgcgtc atattaatgc ctccatgcgg
     2401 gaatcatcgc agcgccaggg attacttgtg gaggcactgg acggaataga aaccctgaag
     2461 ctgaataatg cagttaactg ggtccagcgc cgctgggatg gatatacagc cagtgtcgcc
     2521 cgctcgtcca tacgggtaaa aaataccagt aatgtcataa tctgcttttc cacagcagtt
     2581 cagcaactga atactgttct gctggtcatt ctggggacgt accttattca cagtgatgac
     2641 ccacataacc gaatcaccat gggggcactg attgccgcag ttattctttc cggtcgtgcg
     2701 ctggcaccgc ttggtcagat agcatcactg gccacccgtt ttcagcaggc ccgcctggcc
     2761 atgcaggggg tgaatgacat tgtccgccgt ccggtggagc gggaaagtca gacacattat
     2821 ctgacagtga aggatatcca gggagagttt cgtttcagtg atgtctcctt ttcttataag
     2881 cccggattac ccgacgcatt gtgcggtctg tcgctctgta tccggcccgg agagcgggtg
     2941 ggtattattg gctcaatagg ttgtggtaaa tcgacgctgc tgaaaatgat ggtggggctg
     3001 taccgtcaga acaggggaca aattactctt gacggggttg cgatgcagca actggacccc
     3061 tgttttgtcc gggataaagt gtttcttctg gaacagaatc cccggctttt tctgggaact
     3121 ctgcgtgaaa atctgcagct ttcgcgtctt ccgggaagcc tgtcggatgt ggatatgatt
     3181 aatgcagccg gacgtttcgg gtttgaccgt tttatccggc atcatccccg cggacttgat
     3241 atgcagacag gggaaggcgg tcagggactg tcaggaggac agaggcagat ggttgccctg
     3301 acgagaatgg tactgggttc tccccgggtg gtactgcttg atgagcccac gacaggtctg
     3361 gacctgcaga ctgagtctgt tgttctgaaa ggtctgagcc actggagccg tggacgcaca
     3421 atggtggtgg tgacacatcg tctgcaggtt ctgaatattg ttgaccgtat tgtcgtgatg
     3481 aatgaagggc gggtaatgat ggacggtccc cgtgatgaaa tactgaaaaa actgtcaggg
     3541 aaacagcaga gtggggagag cagagcgata acttcatgag cgtcaaccgg ataagaaaaa
     3601 agagcagata tgtttaacag tttgtggacc gcagggatat gacagaaaaa acggaacgga
     3661 ataaagcgtc gtcagaggtg gtcaggcagt cagacctggc gctgttgaca gatgtacagg
     3721 cagcccttca gcaggaaaag catcatgccc ttttcctgat ggtgattttt ctgctggtac
     3781 tggtagtgac gtttgttatc tgggcatgga acagtcctct ggatgaagtg acccgaggtc
     3841 aggggagtat tattcccggc agcagggagc aggttataca gacccttgac ccgggtatac
     3901 tgaaatcact tgaggtcagg gaaggcgata ttgttgagaa aggtcaggta ctgctgacac
     3961 tggatgatac ccggagctct gccatgctga gggaaagtga agcccgggtg aataacctgg
     4021 aggccgtcag ggcaagactc cgtgcagaag cttattccga atcactgaca tttccggata
     4081 acgttcctga ggatttgcgg gagcgggaaa gtacggtcta ttttctccgg aagacggagc
     4141 tggcgcagag cattgccggt ctgaagcaga gtaaggcgct gctggacagg gaaatagcaa
     4201 tgacgcggcc aattgtgcgt gaaggggcaa tgtcggaggt cgaactgtta cgtatgcagc
     4261 gtcagtcagc ggagctgcag ttacagatgg atgagaaaca gaataaatac ctgacggaag
     4321 ccggtgctga actggtgaag acagaagcag agcttgctca ggcaaaagag aatatggctg
     4381 gcagagccga cccggttgag cgttcacgtc tcagggcccc cctgagaggt atcgtaaaaa
     4441 atatacgggt aaacacactg ggaggggtgg tttctgcagg acaggacata atggaaatta
     4501 ttccgctgga agaccagttg ctgattgaag cttatataaa tccacgggat gttgcctatg
     4561 tcagaacagg gatgccggca cttgtaaaac tgacggctta tgactatgcc atttatggtg
     4621 gtcttgacgg cgtggtgaca ctggtaagtc cggatacttt acgtgaccag aaacggccgg
     4681 gagatttaaa actggacccg aatgaagctt attaccgggt actggttacc accagtaata
     4741 attatctgac agacaggaac ggaaaaagat tgccagttat tcctggcatg atagccagcg
     4801 tggatataaa aaccgggcgt aaaacggtat ttcagtatct gattaaaccg ataaccagaa
     4861 tgaagcaggc acttcaggaa agataataaa aaggtcttcc ggggaaagta ttttaaatca
     4921 attttaaacg ggcagaatga tttcacttta ttctgcccgg gtcattgcgc atgcctgtat
     4981 gtgatttggg gctgaagata atataaatgt tatatctgtc aggggaaaat aaatctgaaa
     5041 aaagcggaat gattaaggag gtccggtcag agaaataaat tatctgtaat ctgattcgtt
     5101 tttatttaat gactcccttt agcggataaa acataagagc atactggttt tattaacctg
     5161 aggatactta tatgaaaatg attttcaccg gcaaggtcag tggagaaaaa acagtactga
     5221 cagccggagc ccgtcataca gtaaaggcac aggccgggga acagtatgga ctggttgatg
     5281 aggtgacagg tctggtcccg gatggtgtgg aagcggaccg ttctggtgat gatttaattc
     5341 tgcgtaaaaa ggaagatgat acagaaatcc ggattgaggg attctgggaa gaatgccagc
     5401 cgggtgaaac acagtgtaca gcagtatt
//
LOCUS       DAZKJR010000234         5157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKJR010000234 DAZKJR010000000
VERSION     DAZKJR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.235"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            338..919
                     /locus_tag="ACHOWS_004947"
                     /pseudo
     CDS             338..919
                     /locus_tag="ACHOWS_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            1006..1284
                     /locus_tag="ACHOWS_004948"
                     /pseudo
     CDS             1006..1284
                     /locus_tag="ACHOWS_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1552..2556)
                     /locus_tag="ACHOWS_004949"
     CDS             complement(1552..2556)
                     /locus_tag="ACHOWS_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0050401.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTTNQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
     gene            2605..3560
                     /locus_tag="ACHOWS_004950"
                     /pseudo
     CDS             2605..3560
                     /locus_tag="ACHOWS_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3600..4079
                     /locus_tag="ACHOWS_004951"
                     /pseudo
     CDS             3600..4079
                     /locus_tag="ACHOWS_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            4222..4451
                     /locus_tag="ACHOWS_004952"
                     /pseudo
     CDS             4222..4451
                     /locus_tag="ACHOWS_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4438..>5157)
                     /locus_tag="ACHOWS_004953"
     CDS             complement(4438..>5157)
                     /locus_tag="ACHOWS_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFQ0050402.1"
                     /translation="ALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHTVFLKKGSF
                     ASRKTDDMLLLQETERALADKSSPKVIFLHMIGSHPNPCDRLNSWPNHYLEQYPRKIA
                     CYLASISKLDNFLGQLDGILRRHSRHFAMLYFSDHGLSVSDSANPVHHDGHVQGGYSV
                     PLIITASDITSHQSVSRKISARHFAGIFQWLTGIRTENIPPFNPLTDEDNEPVMVFNG
                     ERNVPADSLKPQPLILPVRGK"
BASE COUNT         1413 a         1187 c         1230 g         1327 t
ORIGIN      
        1 gtaagttaag ctgagaacac gttattctga ttttcactca cttcacgatt attcctccgg
       61 cctgcaggtt cggaggattc ttatttttat gttttatttt cagataaagt ttttgacaaa
      121 acgtttattc tgcatcagca aaattatcac aaaacaacgc aatcacagac aaattaacat
      181 ttttcagacg ggtatattga gtgcccacaa tgtaagacag gcaggcatcc ggtgacatgg
      241 taaaatatta atccagggca aaaacaaggc ttgatacaca acatcatgac ttttatgctc
      301 tcagactatg ccaccacaga ttttacaggc tcagaaaatg aatgacagat tacgctttga
      361 agtgaacgat aaccagggct gtttcgtgtt cccggacgcc tggtttggct cgttgctgga
      421 tgagtttgac gaactaattg atgcatacga cagagatgaa atcagcgaaa caagttatat
      481 caacaaactc agacgccttg cccgacagga gcctgacttt attgatgtac acgcttacct
      541 ggcatgcgca tttctggagc agaatgcccc gcgtaaggca ctgaacgctg ctcttaaagg
      601 actggcagca ggtaatcgtc tcatcccgga aagtttcagt ggtgaaatca tctggatgca
      661 tcccgaaaac cggccttatc tgcgcgcgct ttatgcagca attctcgcaa atgtgcactt
      721 acagcgtcat caggatgcag ctctgctcac tgataaaata ctggcataca atcctgaaga
      781 caatcaggga gcacgctggc tactgggacc ggaactgtta cgtactggcg accatgaaca
      841 ggcacgccat gtactgaaag cgcatgcaga tgaattttca ccttactggt atgaactcgg
      901 ccttctgcat tttctgaacc ggccccttga aacgccagac agtggatgta tcttaaaata
      961 agagataggc ttggatatat gtctaacact agttctaatt ttgagatgac tggaattttg
     1021 ctggggcagg agcttcgtaa acgtaaaact cctcaggaaa aaatcgccat tattcagcag
     1081 acaatggaac ccggtatgac ggtgtcccat gtggcgcgtt tgcatggtat tcagcccagc
     1141 ctgttgttta agtggaaaaa acaatatctg gaaggaagtc tcactgctgt tgctgccgga
     1201 gaggatgtcg ttcctgcctc agaactggct gccgctataa aacaaataaa agagttacag
     1261 cgcttgctgg gtaagaaatc tatggtcccc accatttttg caacactgat tttgattgat
     1321 gttggcttgc ctaaatctat ccggcgtcat tatgggcatg gatgcccgcg cctcgatgag
     1381 ttccgcacct gatgagccca aaaaaccatc cggcttcagt gagccatttt cttgctgagg
     1441 cttttcatca ggccgatatg ccgttcatgt catcaataat cagtcttcgc aaaaccagat
     1501 tgggaactac tttaattcat gattaacctg ccagccagac tggcgccatc gttacctttc
     1561 gaacacagtc tgctgtgttg tgattgccca cgctatccgg gctaatttgt tggccagtgc
     1621 gcaacatact acgctggagt gttttcttgc caacaacgcc ctgacccaat ccgccaacct
     1681 tcctgactga ttctcgattc tctgaataaa ggatcttgcg cactgaacga gtaaccgtcg
     1741 cagattttta tctccacgtt tacttatccc taaaagtgta tttttaccac ctgtgctgta
     1801 ctgccggggt accagaccgg ttgaagcagc aaagtcccga ctgcagctat attgtttacc
     1861 gtctcccagc tgcgatgaca gtacgctggc ggttatcgga ccaataccgg gtattgtctg
     1921 aagacgttgt cctacctcat cacgactcag ttcctgctcc agtgcgattt caagctcggt
     1981 gatctgtccg acaagataaa tataatgggc atgtaagcgc atcagtaact gcgccagata
     2041 gggaggaagt tcgttctctg caagtactgt tgaaagtcgt tttaccactg cgactcctgt
     2101 cggcatactg atgccaaatt ccagcaaaaa agcatgcatc tgattggtcg ttttgacttt
     2161 atcactgacc aatgattccc tgacccggtg aagcgcccgc atggcctgct gagcttctgt
     2221 tcgtggttgc acaaatcgca tggagggacg agatgccgct tcgcatatgg cttctgcatc
     2281 gacaaaatcg ttcttgttgc tcttaacaaa agggcgaacg aactgcggag aaatcaattt
     2341 tgcttcatga cctaaatcag caatccggcg agccataaaa tgagctccgg cgcaggcttc
     2401 catcacaacg gtagacgagg cacagcctgc cagaaactca ataagtttgg tacgggtaaa
     2461 ctttttacgc agcaatacct tgcctgactt atcttggcag tgaatgtgaa aggaatgctt
     2521 gccaagatct ataccaataa gcgtaatgtt ttgcatgatg gtcttctcca tgaaagaaaa
     2581 acactctact agggtacagc tcacagggtg gtggggacca tctcattaac catggaagtt
     2641 gagatcctca aagaagcggt ggagtatggt cagtcgcgaa aatggatagc gcatgcgccc
     2701 ttgttgccaa cggacaagga ataaccctgg taagccgtat tctgggcgtg tcgcgtgcgc
     2761 aactggcaca acggatgaaa acatctggta ataagccgaa taaacgtcgt cgcagatgtg
     2821 atgaggctgc ggatgcggaa gtgttagcac gtatccttga tatcatcagc gatatgccga
     2881 catatggcta tcgtcgtgtc tgggccattc tcagacgaca gtcccgtaac aaaggagtgc
     2941 cgttcgtgaa tgcaaagcgc gtttatcgga taatgagcga aaataacctg ttgttacatg
     3001 acaaaccttc acggccgcag cgggaacata aaggccgaat atcggtgaag gaaagcgacc
     3061 agcgctggtg ttccgatggc tttgagttcg gttgtgatga tggcgcaaaa ctgcgtgtca
     3121 cgttcgcact ggactgttgc gatcgtgaag ccatagactg ggccgcaagc acgggaggtt
     3181 atgacaaagc aacggtgcag gatgtcatgt taggagcaat agaaaaacgc tttggcgata
     3241 aaggagcgga acggccaatc cagtggctga ctgataacgg ttcggcttat acagctcatg
     3301 aaacacggca gttcgccaga gagttgaatc tggagccctg catgacggca gtcagtagcc
     3361 ctcagagcaa tggaatggca gaaaggttcg tgaaaacgat gaaagaagac tacatcgcgt
     3421 tcatgccgaa accgaatgta agaacggcat tgcataacct tgcagtggcg atcgagcatt
     3481 acaatgaaac ccatccgcat agtgcgttag gttatctctc accaagggaa tatcgacgtc
     3541 agcgggtaac gtcaacttaa gatacaacga ctgtctgaat atcgtgggtc aaggacagcc
     3601 ttctgcattt tcttaatggg gaactggtaa aagctgcaac tgcatttcgt cgcggctttg
     3661 cggcaaatac ttacatcgca gaaatactgt gcggaaatct gcatccattt ccacttgctg
     3721 tatggcataa cttttcaggc ggtccggata ctgctgaaga ttattatgcc acataccacc
     3781 cgttatgggg gcaatatccg gaagcattac tgtttgtaaa ctggctctat aatcattcct
     3841 ctgtgctgca tgaacgggca gaaatcatta aatgcgccga aatgctgatg caggaagatg
     3901 atttcgaaat atgcgaaagt attttaagac agcaggagaa gttgcgtgaa agaattgatg
     3961 agacgctttc tgagaaaatt gtacagaaat gcagaaatat gaatggtgaa tatgtctggc
     4021 cctggatatt gccgttttca gcggcaggca tgaaacatac tggcatacag tatcagtaga
     4081 tattgcatta gtgtatcctg cacacaagta ataatttatc caccaataat aacactgtta
     4141 atgtcccctt cccctggttg tcagccaggg gttatcttct gaatatttct tttgaaaagg
     4201 ataacacagt aaattatttt tatgaattat cccatggact cattaacacc ctttcataat
     4261 gctttattgt caaacacgtt atggctgaca tcaaaaaaac cggatttcct ctgccagcgg
     4321 gtaatcacct ccccggtgtt ttcggttggt ctggttactc cagtctggta attagcaaga
     4381 taatcgatat aaacagtgga aaactcatcg tacataatct ggtgatgaac attacgctta
     4441 ttttcccctg accggaagaa tcagaggctg cggtttcaga ctgtctgccg gtacattcct
     4501 ctccccgtta aaaaccataa cgggttcatt atcttcgtct gtcagcggat tgaatggcgg
     4561 tatattttca gtacgaatgc cggtcagcca ctgaaaaata cctgcgaaat gacgggcact
     4621 gattttcctg ctgacggact gatgagacgt gatgtcactg gcggtaataa tcaggggaac
     4681 gctgtagcct ccctgcacat gaccatcatg atgaacagga ttggcactat cgctgaccga
     4741 cagcccatgg tcagaaaagt aaagcatggc gaaatgacgg gaatgccggc gaaggatacc
     4801 atcaagctga ccgagaaagt tatccagttt actgatgctg gcgaggtaac aggcaatctt
     4861 tcggggatac tgctccaggt aatgattcgg ccaggagtta agtcggtcac acggattcgg
     4921 atgagagcca atcatgtgca ggaaaatcac cttcggagag gatttatccg ccagcgcacg
     4981 ttctgtttcc tgtaacaaca acatgtcatc cgttttacgg gaagcaaagc tgcctttctt
     5041 gaggaaaacg gtatgctccg catcagaagc aataacagag atgcgtgtat catgctcccc
     5101 cagttttccc tgattggata tccaccatgt gctgtatcct gcttttgctg ccagcgc
//
LOCUS       DAZKJR010000235         5078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKJR010000235 DAZKJR010000000
VERSION     DAZKJR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.236"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..795
                     /locus_tag="ACHOWS_004954"
     CDS             <1..795
                     /locus_tag="ACHOWS_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFQ0050403.1"
                     /translation="LLKETDGKQRYEAWLMWLCDTFSVESGYAGLSFALPYARERMFP
                     CEFALAQRFSGVMVDSLGTLEGGEAVEGLKGACWYTILGTPWLEKLGGAERVKHRLAK
                     TPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSPLLVKVNQVIRSIRYDGRNGLHFYS
                     EYEGLQFEEASSMAWFARFDEASALLDKEEMGKSCAPERIKCWTDEIAPHAGQWAAMV
                     NGTTEYIQTREGQKMPPFEDKHGKQHRACWKLLKRDDNGSVYTLPE"
     gene            809..1162
                     /locus_tag="ACHOWS_004955"
     CDS             809..1162
                     /locus_tag="ACHOWS_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFQ0050404.1"
                     /translation="MTVQAGERLGLFARTGQLSLKSGEGAVDVQAQNGSMRLFAEKKL
                     TLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTMATYLRRTKRTMVAAPATMPLP
                     IPLLLDLYPPLNGQI"
     gene            1491..1946
                     /gene="tssE"
                     /locus_tag="ACHOWS_004956"
     CDS             1491..1946
                     /gene="tssE"
                     /locus_tag="ACHOWS_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFQ0050405.1"
                     /translation="MSHHSPSLYETLYGNFTGGLDLHQVCEENQVIVSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTTILQGMPGTAHQLMRVLSDVLLKYEPRIKRVDVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRHLKQQQFVDNSSYYHV"
     gene            2014..2319
                     /locus_tag="ACHOWS_004957"
     CDS             2014..2319
                     /locus_tag="ACHOWS_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050406.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRVFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            2374..2688
                     /locus_tag="ACHOWS_004958"
                     /pseudo
     CDS             2374..2688
                     /locus_tag="ACHOWS_004958"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            3013..3162
                     /locus_tag="ACHOWS_004959"
                     /pseudo
     CDS             3013..3162
                     /locus_tag="ACHOWS_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3337..3738
                     /locus_tag="ACHOWS_004960"
                     /pseudo
     CDS             3337..3738
                     /locus_tag="ACHOWS_004960"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            3720..3944
                     /locus_tag="ACHOWS_004961"
                     /pseudo
     CDS             3720..3944
                     /locus_tag="ACHOWS_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            3947..4233
                     /locus_tag="ACHOWS_004962"
                     /pseudo
     CDS             3947..4233
                     /locus_tag="ACHOWS_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4255..>5078
                     /locus_tag="ACHOWS_004963"
     CDS             4255..>5078
                     /locus_tag="ACHOWS_004963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050407.1"
                     /translation="MKRISVNVLDLYLDSKNPRHDPINNRMEIINYLIQNEKIKVLAK
                     SIAENGTSPLETLAVMENENGHFIALEGNRRVCALTLLNDPDLCPENERAYFRKLSDN
                     SDVIPDAVECVLFENREDANKWIELRHNGEQDGAGLLTWNATQKARHFGSSDNTLALK
                     LLDYAETKGFISKQKRDGILTTASRFLGNPVFRSTMGIRTGRSESNVMIDVSRDEFDK
                     VLERFINDLINGENNVTSRSKKEDWENYAKQLRSEGIAPKGHCEPFALFSTEHSNAS"
BASE COUNT         1385 a         1103 c         1316 g         1274 t
ORIGIN      
        1 ctgctgaaag aaactgatgg aaagcagcgt tatgaagcct ggctgatgtg gttgtgtgac
       61 accttttcgg tggaaagtgg ttatgcaggg ttgtcgtttg cacttcctta tgcccgtgag
      121 cgaatgttcc cctgtgaatt cgccctggcg cagcgttttt ccggtgtgat ggtggattct
      181 cttgggacgc ttgaaggcgg tgaagccgtt gaggggctga aaggtgcctg ctggtacacc
      241 attctcggta cgccgtggct ggaaaaactg gggggggcag agagagtaaa acatcgttta
      301 gctaaaacgc ctgaaattag cctgctaccc tataacaatg gtgtcgtact taaagccggt
      361 gaactccccc cgccactggg agagatgaaa acagaaggtt tatcgccgct gctggtgaag
      421 gtgaaccagg tcattcgttc cattcgctat gatggacgca atggtctgca tttttactca
      481 gaatatgaag gacttcagtt tgaagaagcg tcttcaatgg catggtttgc ccgttttgac
      541 gaagccagtg ccttgctcga taaagaagaa atggggaaat cttgcgcccc cgaacgcatt
      601 aaatgctgga ccgatgaaat agcgccccat gccggacaat gggccgcgat ggttaatggc
      661 accaccgagt acatccagac ccgcgaaggg cagaaaatgc ccccgtttga ggataagcac
      721 ggtaagcagc acagggcttg ctggaaacta ctgaaacgag atgacaatgg tagcgtttat
      781 acactacctg agtaaggtcg tgtgctgaat gaccgtgcag gccggagaga ggctggggct
      841 ttttgcccgc accggtcaac ttagcctgaa atccggggaa ggggcagtgg atgttcaggc
      901 gcaaaacggc agcatgcggt tgtttgctga gaagaagctg acgctgagct cagcgagtga
      961 catctccttt gccgggaaaa aacgcatcac gctgattggc gggggaagtt acctacggct
     1021 ggaggcgggg aaaatagagt acggtacgat ggcgacgtat ctgcggcgga cgaaacggac
     1081 gatggtagca gccccggcaa caatgccgct ccccataccg cttctcctgg acctgtatcc
     1141 accacttaat ggccaaattt gaattgaatg tctcctcaat gccatcaaag tcaacgatgc
     1201 tatcgttcag ggagcgtagt caatggatgt cacaaaaatg atgcaggtgc tacaggattt
     1261 gtttttatgc tgctaaatgt acagcgactt cgtgatatgg ggttgttggt gttttttata
     1321 ataatgcttt ttacggaatc aaaaaaacag acaacatatt aattatttga gaaagcagcg
     1381 tcaaaaagga aaagttaaaa tgtcgaatcg attttttact ccggtaatct tctttccagt
     1441 gtgttgattg aactatttcc aagattaaat gattatgaga agaagtcgtg atgtctcatc
     1501 atagcccctc cctttacgaa accttatacg gaaattttac cggcggcctc gacctccatc
     1561 aggtctgtga agaaaatcag gtcatcgtgt cggtgctcga taacatgcag cgtattctga
     1621 atacccgggc cggaagcctt aaacatcttc cggactatgg gctgccggat atgacaacca
     1681 ttctgcaggg aatgccgggt acggcgcatc aactgatgcg cgtgctttca gatgttctgc
     1741 tgaagtatga gccacgtatt aagcgtgtcg acgtgacgat gcaggaacag actcagcctg
     1801 gtgagttgca ttatgtcatt gatgcagaac ttaaagatgc cgggctggtt cgttacggaa
     1861 caacatttat gccggagggc agagtgttgc tgcgccatct taagcaacag cagtttgtgg
     1921 acaattcatc ttattatcat gtctgatttt tttatccggg ctgcccctgt gtgggtggtc
     1981 cggatgaaaa atatattttt tgtgattttg attatgaacc cgttatttaa cgatattcag
     2041 atgcgtcttt tttatctgaa ccactctccg tactcctggc actggaatgt cagattcaga
     2101 cctcaggaag ccgtatatat tggcagcgat acctgtcata tcaccattac ctgtaatcag
     2161 tccggttttc atttaacccg ggacggacaa cgggtattca ctgagcgtta tatcagaaac
     2221 cttaatgagc tgttaccagt tctgaaacgg cggtgggatg tgacaccggc aatcatccgt
     2281 gcagtggaat acctttcccg ggtcccggta tcacactgac attctttccg taactgttaa
     2341 tcatgattta atacagcaac actgaatctg ccgatgactt ctgaagtgaa actgaaaaca
     2401 ggtggtgacc cccggtcatt cccggattat gctgccctgc gcgatgaaat cagcaaactg
     2461 acccacccgg cacgcccgga tgtggactgg cgttatgtgg aaacgctctg cctgcgcctg
     2521 tatgaacaca atggcgtgga attacagaca gcatcctggt acaccattgc ccggatgcat
     2581 acaacagggt taagtggcct gaatgaaggg ctggctctga ttgtggcgct gacccggcat
     2641 cactggtcag tgatgtggcc gctgaatacc cacgcccggc tggaaattat taccggtctg
     2701 ttcaaccgtc tgcaaaaaac gctccgggcc atgccaccgg acgaccgtga caacctgccc
     2761 ctgctgtatc agaccgaaac attcctgaag gcactgagtg atacactggc ccggcacgaa
     2821 cttaaacaga gcagtaaagt ggctttaccg gaaaccatgg tgaagggata tatcacccgt
     2881 ctggaaaatc agccgatgca gggcgaagtg tcgtctccgg taacccttcc tgctcgggca
     2941 ctccggtctg atgctccgga tgtacaggga catcagcccc tgccgcactc ccgtctggtg
     3001 tatattgttt ctgaaacaaa agcagagccc acaccctccc ttcaaaaatc cccgcccacg
     3061 tttctgaaac cgtttgtggc tggtgtctgt gcggcattgc tggcggtcag cgtggccatt
     3121 ccgggatggc agtttctgac acagccatca ccggcggagc agcaactcag ggcgctgctg
     3181 gcaccactac cggatatgct ttcagcagac cagatggcac agctcagcag aacaccctca
     3241 ctgttaaatc atgcgtcaga ctggataaca ctttcaggtc agcagataac gcgactgaca
     3301 gaactaccgc tgacatacaa cctgcagcgt agtgcgcagc tgttgcagca actgatggta
     3361 cttttccccg ataatcctcg tgtacaggaa atggtggata actggcagaa gagcgtaagg
     3421 agtcgtgcgc tcccggaaga ggcgatgacg ggctggaatg aaggcatgac ccgcttacag
     3481 cagctggctg aacgcctgaa ccgcctggat gaacagcggg gaaaatacat gacggtcagt
     3541 gaactgaaaa cggaggtgtt tggcatcatg caggctttta accggcacat accggcagaa
     3601 gagcagttac gtcgctatga tgaagtccgt aatcagaatg gcagcgaatc tcagcagaaa
     3661 ctggcgcagg atgctctgat ggaacagttg aaccgctact ggcttctgag acatggagat
     3721 gctgggaatc cggcgtgact tgggttcgcc aaccaccgga agacgccatt acggatatgg
     3781 cgtattttcc cttttaccgg tatgccatgg cctttgaagg attcattccg gcattttcat
     3841 cttggtttgc aggtaaccgg tgtggagtgg caatactgac cggtgtccgg gcggatgaat
     3901 ccctcaactg ctttatgggg ctggtgtctc agcgcaaact gagagaatta cgtgggtagg
     3961 atcagatgat ggtattggaa ctgaaggtga tatgggaaaa tggttgttat tattttgtct
     4021 gctgtagctc agtctgaatg acaacatata cttgaaagaa caaatgaagg ccgtctcgaa
     4081 gccgaagtaa agggtattta atttgggcga aaaagaacgg tcaacaggga acaagtacaa
     4141 accttacgaa gtctgggtat cggcgctaca gaaatttcgc gaaagatgaa atttacacgt
     4201 tctactgtgt ataaaatatt agagggtaaa taatattttc atatggaggg ttacatgaaa
     4261 cgtatttcag taaatgtttt agatttatat ttagatagca aaaatcctcg acacgatcct
     4321 attaataacc gcatggagat aattaactat ctgattcaaa atgaaaaaat taaagtattg
     4381 gctaaaagta ttgctgagaa tggaacaagt ccattagaaa cacttgcggt gatggagaac
     4441 gaaaatggac actttattgc ccttgagggg aataggagag tttgtgcatt aactctgctt
     4501 aatgatcctg atttatgtcc agaaaatgag agggcttatt tcagaaaact atctgataat
     4561 agtgacgtca ttcctgatgc agttgaatgc gttttatttg aaaatagaga ggatgcaaat
     4621 aaatggattg aattgcgcca taatggagaa caagacggtg cggggcttct tacttggaat
     4681 gcaactcaaa aagcaagaca tttcggttct tctgataata cattagcgtt aaaattatta
     4741 gattatgctg aaactaaagg atttatttcg aagcaaaaac gagatggcat tttgacaaca
     4801 gcatcgagat tcttaggtaa tcctgtattc aggtcaacta tgggaattag aacagggcgt
     4861 tcagaatcta atgtgatgat tgatgtttcc agagatgaat ttgataaagt tcttgaaagg
     4921 ttcatcaatg acttaattaa tggtgaaaat aatgtcacat caagatcaaa gaaagaagac
     4981 tgggagaatt atgcaaaaca gctaagatcg gaaggtattg cccccaaagg acactgtgaa
     5041 ccatttgctc tttttagtac ggaacatagc aatgcttc
//
LOCUS       DAZKJR010000236         5063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKJR010000236 DAZKJR010000000
VERSION     DAZKJR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.237"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..536)
                     /locus_tag="ACHOWS_004964"
     CDS             complement(<1..536)
                     /locus_tag="ACHOWS_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0050408.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFQRLKDRKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPP"
     gene            1378..1572
                     /locus_tag="ACHOWS_004965"
     CDS             1378..1572
                     /locus_tag="ACHOWS_004965"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFQ0050409.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(3128..4144)
                     /locus_tag="ACHOWS_004966"
     CDS             complement(3128..4144)
                     /locus_tag="ACHOWS_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0050410.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLAERPAARWLFMTLTVKNPPVEDTRETLIRMNAAWNRLVRRKEFRSVLGWLRTTE
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLIGADIPAELDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT         1397 a         1351 c         1085 g         1230 t
ORIGIN      
        1 gggggaacca tcagcagagt gtgaaaatgg ggatgtgcgc taccgtcctt gccgcgcgtt
       61 acttccgtgg tacgcaccca tcccagcaca tgacgaaatt ccttgcgatc tttcagccgc
      121 tgaaaaccag cattcatcgc ggtcagcgtc tcaccaagcg cgtcaatgga acagttgcgc
      181 accgtcagcg tgagaaacaa ccaacgcgcg ccgggatgtt ccgtcacaat ctttggcagg
      241 gactgataga aacgggcttg ccacatcatg gagcgcctcc actggcagat cggacagtgc
      301 cggacacgac agaaccgcgc atcacgcaac cgcaggcgcg tttctccggt ctctgtgttt
      361 gttgaccacc caaaccgcaa aataccacta caagccccga cacgttcgcc gtagtgctca
      421 aatccggcag cacgcagata aataccgccc acatcgtcgg aaaagctccg gcgctcatcc
      481 cacggtttat cctccgggga ataatccgtc agatttttaa gttcaggatt tttcatgtct
      541 gccgttccct agtgagcgac agacgaaatt acgtgttatc attcctgtat cacctgtgtt
      601 gtcgtgtcac caattcgaat tcctacaggt ggtcacaccc ccgaacgacg cccatcgttg
      661 cgggggtcgc ttttaatgcg accacttccg acaaaatact ctcaaaccta tttccacaca
      721 aatccgcgct cagcccagat gtgacaaggg ctagagctaa attacaatcc cagagatatc
      781 ccttaagtat atatacaagg atcgcgcgca cgatcaccca ccaaaaatgc gcgttttttg
      841 agcgcttttc cgcacaacaa aacaacacac aaaaaccact ctcccgacaa tactccagta
      901 ggttttctca ccgattttcc gaacaacaaa accagctttg caggtggttg gtcccactgc
      961 gtgacaccaa ccaccatcac cagtgttttt ttctcgtgcc gtcgtcaatc acaaaatcgg
     1021 aacccacgcc ggaggcgcgg gaccccacga ttttctgttt gacgagtcag aaaacggcga
     1081 acgctgacag atttcaatct ggctgcgtga gacgttggcg gggtgtcggg gcgcagccct
     1141 gaccaggcgg catttgtctg attgcgtgtg cgcggtccga caaatacaca tcctgtcccg
     1201 tttttcgcac ggtttttatt gattaccgga cagatgaacc cggattgcca tgttgcgaaa
     1261 atcacgttaa aacgattacg agcgtgcagt tgggctgata tatcaacagt catgccaata
     1321 tgtattaacc cgacaggccg ctctttttta attcaaataa aaatataaat tacgagtatg
     1381 aatcaggaca aagcaattta tttagaaaat aaatattttt cacccgcaaa cgaagctgtc
     1441 agactggagc gtgaaggcga ttacattgga gcatggaaaa aatggaatga tgcgcttcat
     1501 tatgccaaag gcgaaaatat agactggtgt atagccagag ctgaatattg taaaaagaca
     1561 ggccggtttt aattctgtcg gggtttcaaa ggggcgtccc ctttggcacg tcgacgtatc
     1621 aaaatgcgtc agcattttgt atacagtcgg taacgtgcct ccaggattta ccatcaaaaa
     1681 cgcccctgta gttaatcctg aatcaagata taactaagat ataattagcg tataactaag
     1741 gcataactaa agtataacta acttagtgtg catataatta agatataact aatatataac
     1801 taatgtataa ccaacggttc accaacggat cgcatgtagt agacgctaca taaaaaatcc
     1861 tgaaatggag gattcgtcat aaagagattc ctgtttttag cgttctgatg caggataggg
     1921 caaacaccgc aaaatgacgt ctttggtatg ttttgtgatg tttgcttctg gcatctgtta
     1981 tctgttttat tgggtgtttt tatgagtgtt gatgtaatta aacaaattca gagtatacag
     2041 gacaaaggaa ttattattta ttcaaaatac agagctaacg aatttaacag ggatgatgta
     2101 tacagagaga gttacagcct gattattgag tttaatcatc ttattgctga acatgccgtt
     2161 catgatgaaa aactgattaa ccatactgtc tgtattctga atgaattacg aagcatagcg
     2221 ataaattaaa aaatccccct ggattacagg gggaagccat cagagtttga gtaatgagaa
     2281 atattatttt ggagagagca ccacaaatgc gctcttttcc aatctgccgt aaccatcggt
     2341 aaaagacaat caggacaggc gaaaaaatcc ccctatatgg caagggggtg tcaatgagga
     2401 catggcaatt gtgagttttg tgtgttttat gtgttgcagt ttgcaacgtc agaaatttgc
     2461 cgtagttata cgtgaaaaac aatcctgatt tctggacagg atgtgtaatc gtataaccgc
     2521 gcacgcacgg ctatacgatt accacctggt caggtctttg cccgacaccc tcgacaccgt
     2581 tcccgcgata cgatagaaca tcagggcgcg tttccgggta aacgcatgtt aatgaccggg
     2641 aattcattga ttagggaccg gagacgcgct acaaaactcc tgcgcgtttc tggcatcaac
     2701 tgaccatggg gatggtttgg cagtagcagc aagcgccaga aacgcgctca aaatcatcag
     2761 atgtacagcg aacaaaaaat tgtcatcagc aaaaaatcac gttaaaacaa tcgcgagcac
     2821 cttcctgtct ccttcaatta cggtcaaaca ttttgcatct gtcgggaagt tgctcacctc
     2881 cataattcaa tttctcattg acaataattc cggtgatatc ccctcttttg aggggctttt
     2941 tttacccaca caccacaaac agcagccata ccatcgtcaa aaacgcgctc aaaacgtctc
     3001 tgacgtcctg aaccaaccaa aacatacaaa accattgccc aatgcaaaaa actgcgttag
     3061 aaagcgtctg agcgcgtttc tgacgtcttt tttgttgctg tgtcgctact cgttgtcaga
     3121 ttcgcgctca atccttcatc ctgtcagctt caggattccg cttgtaacgt ttttccgtac
     3181 gtccccagtc aaacgccagc cagaaggctt gctcctcgaa ctcgtccagt tctgccggaa
     3241 tatctgcccc gatcaaatcc tcgtccgtct ccggtttatc gactttcaga acctctttga
     3301 gacgaccgcc ggttgccacc atgcgaagcc cataggtctg ccgcgccagc tccagaaacc
     3361 acgccggatc acgcacaact tccgaagatt tcaccgtgta tttcatcgtc tcaatgacgc
     3421 ctgactccag cgccgcacga tgcatatcag gcagggttac accatccggc cgtttccagc
     3481 cctgtttcgg ttttaccacc cgaatatcca cacccgcctc ataatcgaca cgaaggcatt
     3541 cactccagat ctccacccag cgagcatgtt tcacataacc attaccggac aacatcgaag
     3601 gggggaccat catcagaacg tgaaaatgcg gatggcaaca tcctggcacc ttcccgtaag
     3661 taatttccgt cgtacgtaac cagccaagaa ctgacctgaa ttctttacgc cgaaccaacc
     3721 tgttccacgc cgcattcatc cgaatgagtg tttcgcgggt atcttccaca gggggatttt
     3781 tgacagtcag tgtcatgaac aaccagcggg ccgcaggacg ttcggccagc agctttggca
     3841 aggcctccca gaggcgacgc atccagcgga gagagcgacg acgactacac accggacaaa
     3901 acggaacatg acaaaactcc cccttccgaa gggccagctt catttcgccc gtcaggcggg
     3961 acaccttcgg cgcaaacaac aaccgacggg aacaatcaaa catccgttct ccacgagagg
     4021 aaaacatcga atcgctttcg taaatctgcg ccaccctgtc agccatcact cgatcactgt
     4081 cccattttgt atcccgcgtg ctgtaatcgc gtaacgagag taccggactg tctggatttt
     4141 tcatttgcac cccccaagaa ctgggcagat gcggaaattt tgtgttagca tttttctgtc
     4201 acctgtgact ctgttgccga acaacgaatt ccaaactggt gatcgtcccc tggagtgtta
     4261 ccgcactcgc aggggaaccc cctcaaaatc gttcattttt taaccagaag tttttatctc
     4321 ttttccaccc atttttaccg ctcaaagccg cgccaccact gggctaagcc aaaataacac
     4381 atccttgagt tgttgcctat agtataaata caaggatcgc gcgcgcgatc gcccacccaa
     4441 aatgcgcgtt ttttgagcgt tcccgagaac aaaaaaacaa cacatccaaa ccacctgatc
     4501 agcaggtttc tcaccgactt tctgaacaac aaaaccgctt tgcaggtggt tggtcccact
     4561 gcgtgacacc aaccaccatc accagtgttt tttctcgtac cgtcgtcaat cacaaaatcg
     4621 gaacccacgc cggagacgcg ctacaaaact cctgcgcgtt tctggtattt actaaccagg
     4681 ggatggtttg ccgtagcatc gctgtgtcac cagaaacgcg ctcagaacac ctctgacggc
     4741 ctgaaaacaa aaaaacgagc aaaaccttgc ccgcttcctg aaatcgcgtt acaaagcgtc
     4801 tgagcgcgtt tcttcctcgt tcaatctgtt cagttcagtc attccattgc ctagaaaacc
     4861 tctggaaacg cgctcaaaac gtctctgacg gcctgaacca accaaaacat acaaaaccat
     4921 tgcccaatgc aaaaaactgc gttagaaagc gtctgagcgc gtttctggtg agcaaaaagc
     4981 tgtcagccgt ggatttccat tgcagagacc cagaaacgcg ctcaatcatc gctgcttagc
     5041 cgacgtttat ctttcgcccg atc
//
LOCUS       DAZKJR010000237         4969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKJR010000237 DAZKJR010000000
VERSION     DAZKJR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.238"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..1508
                     /gene="garP"
                     /locus_tag="ACHOWS_004967"
     CDS             174..1508
                     /gene="garP"
                     /locus_tag="ACHOWS_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFQ0050411.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1524..2294
                     /gene="garL"
                     /locus_tag="ACHOWS_004968"
     CDS             1524..2294
                     /gene="garL"
                     /locus_tag="ACHOWS_004968"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFQ0050412.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2324..3214
                     /gene="garR"
                     /locus_tag="ACHOWS_004969"
     CDS             2324..3214
                     /gene="garR"
                     /locus_tag="ACHOWS_004969"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFQ0050413.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3311..4456
                     /gene="garK"
                     /locus_tag="ACHOWS_004970"
     CDS             3311..4456
                     /gene="garK"
                     /locus_tag="ACHOWS_004970"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFQ0050414.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4489..4865
                     /gene="rnpB"
                     /locus_tag="ACHOWS_004971"
     ncRNA           4489..4865
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOWS_004971"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1172 a         1178 c         1412 g         1207 t
ORIGIN      
        1 ctcaaaataa tatttaaata gtactaatta aattagcatt tgcacaatga ttattattct
       61 cgcgctgctt atactaattc ccgagttcat tgtcgtaacg gcataaatga aactcataaa
      121 taacaccttc cccataataa taaaaatcag cataagtacc cgaggtaaat aaaatgatgc
      181 tggacaccgt tgacgaaaaa aagaaaggcg tgcatacccg ctatttaata ttactgatta
      241 tttttattgt taccgccgtt aactacgccg atcgtgcaac gctgtctatt gctggtaccg
      301 aagtggcaaa agagttgcag ttaagtgcgg tttcgatggg ttacatcttc tctgcttttg
      361 gctgggccta cttgctgatg caaatccccg gcggctggct gcttgataag tttggttcga
      421 aaaaagttta cacctacagc ctctttttct ggtcgctatt caccttcctg caaggctttg
      481 ttgatatgtt cccgctggcc tgggcaggga tctccatgtt ctttatgcgc tttatgctgg
      541 ggttctcgga agcgccatca ttcccggcga acgcccgtat tgtcgccgcc tggttcccga
      601 cgaaagaacg tggtactgcc tccgccatct ttaactcggc gcaatatttc tcgctggcgc
      661 tcttttcgcc gctgcttggc tggctgactt tcgcgtgggg ctgggagcac gtctttaccg
      721 ttatgggggt aattggtttt gtgctgacgg cgctgtggat caagttgatt cataacccga
      781 cagatcaccc acgtatgtct gcggaagagc tgaagtttat ctcggaaaat ggcgcggtgg
      841 tcgatatgga ccacaaaaag ccgggcagtg cggcagcaag cggacccaaa ctgcattaca
      901 tcaagcaatt gctctctaac cgcatgatgc tgggcgtatt tttcggacaa tattttatca
      961 acaccatcac ctggttcttc ctcacctggt tcccaattta tctggtgcag gaaaaaggca
     1021 tgtcgattct gaaagtgggt ctggtcgcct cgattccggc actgtgtggc tttgcgggcg
     1081 gcgtgctggg aggtgtcttc tcggattatc tgatcaaacg cggtttgtcc ctgaccctgg
     1141 cacgtaagct accaattgtg ctgggaatgt tgctggcttc caccatcatc ttatgtaact
     1201 acaccaacaa caccacgctg gtggtcatgc tgatggcgct ggctttcttt ggtaaaggat
     1261 ttggtgcgct gggctggcca gtgatttctg acaccgcgcc gaaagagatt gttggcctct
     1321 gcggcggcgt ctttaacgtc tttggcaatg ttgcctccat tgtcactcca ctggtgattg
     1381 gctacctggt aagtgaactg cactccttca atgcagcact ggtttttgtg ggatgttcag
     1441 cgctgatggc gatggtctgc tacctcttcg tagtcggcga cattaaacgt atggaattgc
     1501 agaaataagc aaaggtataa gcgatgaata acgatgtttt cccgaataaa ttcaaagccg
     1561 cactggctgc gaaacaggta caaattggtt gctggtcagc tctctctaac ccgattagca
     1621 ctgaagttct tggtttggct gggtttgact ggctggtgct ggatggtgaa catgcgccaa
     1681 acgatatttc cacgtttatt ccgcagttaa tggccctgaa aggcagcgcc agcgcgccag
     1741 tagtgcgagt gccgaccaac gagccggtaa ttattaagcg tcttctggat atcggtttct
     1801 ataacttcct gattcctttt gtagaaacaa aagaggaagc agagcaggcg gtggcatcaa
     1861 ctcgttaccc accggaaggc attcgcggcg tctccgtttc tcaccgcgcc aatatgtttg
     1921 gcaccgtggc ggattatttc gctcagtcga acaagaacat cactattctg gtccagatag
     1981 aaagtcagca gggcgtagat aacgtcgatg ctattgccgc taccgaaggt gtagacggca
     2041 tcttcgtcgg ccccagcgat ctggctgcgg cattaggcca tctcggcaat gcctcgcacc
     2101 cggatgtaca aaaagcaatt cagcacattt ttaaccgtgc cagcgcgcac ggcaaaccca
     2161 gcggcatcct cgcaccagtc gaggctgatg cgcgtcgtta tctggaatgg ggcgcgacgt
     2221 ttgtggccgt cggcagcgat ctcggcgtct tccgctctgc cactcagaaa ctggctgata
     2281 cctttaaaaa ataaccacca ccgcaataag agagatgatt gatatgacta tgaaagttgg
     2341 ttttattggc cttgggatta tgggtaaacc aatgagtaaa aaccttttga aagcaggtta
     2401 ctcgctggtg gttgctgacc gtaacccaga agctatcgct gacgtgattg ctgcaggtgc
     2461 agaaacagcc tctacggcta aagcgatcgc tgaacagtgc gacgtaatca taaccatgct
     2521 gccaaactcc cctcatgtga aagaggtggc gctgggtgag aatggcatta ttgaaggcgc
     2581 gaagccaggt acggtattga tcgatatgag ttctatcgca ccgctggcaa gccgtgaaat
     2641 cagcgaagcg ctgaaagcga aaggcattga tatgctggat gctccggtga gcggcggtga
     2701 accgaaagcc atcgacggta cgctgtcagt gatggtgggc ggcgacaagg ctattttcga
     2761 caaatactat gatttgatga aagcgatggc gggttccgtg gtgcataccg gggaaatcgg
     2821 tgcaggtaac gtcaccaaac tggcaaatca ggtcattgtg gcgctgaata ttgctgcgat
     2881 gtcagaagcg ttaacgttgg caaccaaagc gggcgttaac ccggatctgg tgtatcaggc
     2941 aattcgcggt gggctggcgg gcagtaccgt gctggatgcc aaagcgccga tggtgatgga
     3001 ccgcaacttc aaaccgggct tccgtattga tctgcatatt aaggatctgg cgaatgcgct
     3061 ggatacttct cacggcgtcg gcgcgcaact gccgctcaca gctgcggtta tggagatgat
     3121 gcaggcactg cgtgcagatg gtttaggaac ggcggatcat agcgccctgg cgtgctacta
     3181 cgaaaaactg gcgaaagtcg aagttactcg ctaatgacgt cgtgcccgga tggcgtattg
     3241 caatccgggc ctggaatcca ggccgaataa gagaaacgcc ctccggcgtc atgtggtaac
     3301 aggcataggt atgaaaatcg taatcgcccc agactcttat aaagaaagtt tatctgccag
     3361 cgaggttgcg caggcgatag aaaaaggatt tcgggaaatt tttcctgatg cacagtatgt
     3421 ttctgttccg gttgccgacg gtggtgaagg aacggtggaa gcgatgattg cagccaccca
     3481 gggggctgaa cgtcacgcct gggttacagg gccgctgggc gagaaagtga atgccagttg
     3541 ggggatctcc ggcgatggca aaaccgcgtt tatcgaaatg gcggcggcca gtgggctgga
     3601 actggtacct gcggaaaaac gcgatccact cgtgaccact tcacgcggta ctggtgagtt
     3661 aatcctgcag gcgctggaga gcggtgcgac aaacattatt atcggcattg gcggcagcgc
     3721 caccaacgac ggcggcgcag gcatggtaca ggcgctgggg gcgaaattat gcgatgccaa
     3781 cggcaatgaa attggttttg gcggcggtag tcttaatact ctgaatgata ttgatatttc
     3841 cggtctcgat ccgcgcttaa aagattgtgt catccgcgtc gcttgtgatg tcaccaaccc
     3901 gttagtcggc gacagtggtg cttcgcgcat ctttggccca caaaagggag ccagtgaggc
     3961 gatgattgtc gagctggaca ataacctctc tcactatgcc gatgttatta aaaaagcgct
     4021 gcatgttgat gtgaaagatg tccccggcgc aggagctgcg ggcggtatgg gcgcggcgct
     4081 gatggcgttt cttggtgcag aactgaaaag tggtattgaa atcgtcacta cggcgctgaa
     4141 tctggaggaa catattcacg attgtacgct ggtgatcact ggtgaagggc gtattgatag
     4201 ccagagtatt cacggcaagg taccgattgg tgtcgcaaac gtggcgaaga agtaccataa
     4261 accggtgatt ggcattgcgg gtagcctgac caatgatgtt ggcgttgtac atcagcatgg
     4321 cattgatgcg gtcttcagcg tattgaccag cattggtacg ttggacgaag cattccgcgg
     4381 ggcttatgac aatatctgcc gtgcttcacg taatatcgcc gcgacactgg cgattggaat
     4441 gcgcaacgcg gggtgacaag ggcgcgcaaa ccctctatac tgcgcgccga agctgaccag
     4501 acagtcgccg cttcgtcgtc gtcctcttcg ggggagacgg gcggagggga ggaaagtccg
     4561 ggctccatag ggcagggtgc caggtaacgc ctggggggga aacccacgac cagtgcaaca
     4621 gagagcaaac cgccgatggc ccgcgtaagc gggatcaggt aagggtgaaa gggtgcggta
     4681 agagcgcacc gcgcggctgg taacagtccg tggcacggta aactccaccc ggagcaaggc
     4741 caaatagggg ttcataaggt acggcccgta ctgaacccgg gtaggctgct tgagccagtg
     4801 agcgattgct ggcctagatg aatgactgtc cacgacagaa cccggcttat cggtcagttt
     4861 cacctgattt acgtaaaaac ccgcttcggc gggtttttgc ttttggtgcc cagggtaaga
     4921 tgaatatcta cggtattaat aatagacttt gtaagaagtt attaagtct
//
LOCUS       DAZKJR010000238         4903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKJR010000238 DAZKJR010000000
VERSION     DAZKJR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.239"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(25..222)
                     /locus_tag="ACHOWS_004972"
     CDS             complement(25..222)
                     /locus_tag="ACHOWS_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFQ0050415.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(234..722)
                     /locus_tag="ACHOWS_004973"
     CDS             complement(234..722)
                     /locus_tag="ACHOWS_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFQ0050416.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPTF
                     DW"
     gene            complement(719..1093)
                     /locus_tag="ACHOWS_004974"
     CDS             complement(719..1093)
                     /locus_tag="ACHOWS_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFQ0050417.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(1183..1536)
                     /locus_tag="ACHOWS_004975"
     CDS             complement(1183..1536)
                     /locus_tag="ACHOWS_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFQ0050418.1"
                     /translation="MSDTLPDGNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAG
                     IRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEADTLGSC
                     GYVYMAVYPTPETKK"
     gene            complement(1612..1833)
                     /locus_tag="ACHOWS_004976"
     CDS             complement(1612..1833)
                     /locus_tag="ACHOWS_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFQ0050419.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(1902..2378)
                     /locus_tag="ACHOWS_004977"
     CDS             complement(1902..2378)
                     /locus_tag="ACHOWS_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFQ0050420.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(2394..2879)
                     /locus_tag="ACHOWS_004978"
     CDS             complement(2394..2879)
                     /locus_tag="ACHOWS_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFQ0050421.1"
                     /translation="MTTFSHSSTTPSVSVTTASGSDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(2970..3788)
                     /locus_tag="ACHOWS_004979"
     CDS             complement(2970..3788)
                     /locus_tag="ACHOWS_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFQ0050422.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(4115..>4903)
                     /locus_tag="ACHOWS_004980"
     CDS             complement(4115..>4903)
                     /locus_tag="ACHOWS_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFQ0050423.1"
                     /translation="LEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDD
                     AGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGWGWLGSLETGLPFSI
                     TDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTS
                     SRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSL
                     DLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT         1109 a         1299 c         1385 g         1110 t
ORIGIN      
        1 gtaatgaagt ctggtgagta agactcaccg gttctgttgc agaaactgca gttggcgcag
       61 ggtacggata tcttccctgg cctgttcaat gcagggcagc agtgctgccg aatccgtttt
      121 atcctcatcg ttgtcaaaga taattcccga ttcgcagtcg atattgtcct gcagccatgc
      181 aatcagaata tccagcgctg tttccgtggt taatgatttc atgttgtttc tggttaccag
      241 tcgaaagtgg ggaaacccgg cagacatccg gcactggcat ccagatgaat gagactgacg
      301 ccataacgtc ggatgagtgt gacaaccaga cggcggaacg tttttgacag ccccaggcgt
      361 ttaagccgca gaaccgggta tgaccacgca tcggtacgta acagatatcc gctacctgtg
      421 aaatgaatcc attctgattc accaacgtca ctggtctgat gcgacagcga gtacagccag
      481 gcgttatcct tttccgtgat atgtgcggta ctgcagcgta tgccggcaag ggttgcaaac
      541 ggtggtagtg tgcaggttga ctgttggtcg gattcctcca ccacgcggag tgaataaccg
      601 ttttcagcga ccttgttaat cagttcagtg agattaacac catcgacgtc aacgacaatt
      661 tgccccatgt tcagtgcctg cacattaatt ctgtcggctt ccagcatcag ggaaagtttc
      721 atcgtttctc cggatgctta cccagggtaa tgttatttac cgttctgtaa ttgtcgcggg
      781 tcatcagacc tgttgcccgg cgtgcccgga gaatatcaat gctgttgatt aactgcgacg
      841 aagttcctgc gctgaatccc ggctggtcgg tacgcaccag cgcgtatttt tccacgagaa
      901 aattcaccgc atcacacagt gaaatgcctg cctcaatatg ctgctcaatc acacgttcat
      961 cagcgaacgg tgtgtcattc agcgtaaggc catagtgctg gtccagcagt cgggtgagca
     1021 gtgtctgcca gatttcaaca ggagatgggc gtgaactggc cgactgcccg ggtaatacag
     1081 gtaatgtttt catactgaag attttcctga tatgcaaaga tgaaaaatgg gaaagtggtg
     1141 tggtgaaaac accaggccgg agcagagggt tattctggag agttactttt tcgtttcggg
     1201 cgtcggataa acagccatat aaacgtagcc acaactgccg agggtgtcgg cttcgcaggt
     1261 cagccctctg gcatacagcg tgacggtatg ctgatggcgg ggattcagtt caccgctggt
     1321 gagcatgagt tccagttgtt tcatcagcag cggaaaggcc tggtccaggt ggtacgcatc
     1381 tgcatcgctg aacaggcctc tgataccagc gcggtcggca aggtaatgca accggttacc
     1441 ctcctgcacc agacgggcgc cgaaacaggg cgtcacggtg cagggcagcc cccaccaggg
     1501 gcggtcgtga ttgccgtcgg gaagtgtgtc tgacacgata aaatcctcac agaaaatcgg
     1561 ttaagaatgg tccggtggtg gcgataattc tgctcatcag aatgccctga ctcagttcag
     1621 ggtgacgctc atcagccgga catacgggcc aaaactgtcc ttacggcgtt cagcaaacac
     1681 ggccagcaca ccgggaatat cctgtacttc acgaccggta tacgccccgg cactgccgtg
     1741 ccagcggtac ttaccggtgc agaacggaaa aagacgggag gccggatgct gttggtgaat
     1801 acgcatggct tcaccacggg tgatgatttt catgatgtga tacctctgaa gacagaagat
     1861 aaaagtgaaa acaggtatga tgtggttgtg acggtgacgg gttaaagcag accgtgttcg
     1921 gcaaaggaga aaacctggct gccaccgact atcagatggt ccggcacccg gatatccacc
     1981 aggcccagtg cctgtaccag acgttccgta ataagccggt ctgccttact gggtgtgact
     2041 tcaccggacg ggtgattgtg tgccagtacc acggcagcgg cattgtggta cagggcgcgt
     2101 ttaatcactt cccggggatg gacttccgtg cggttgatgg tgccggtgaa gagggtttca
     2161 ccggcaatca actgattctg gttgttcaga tacagcaccc ggaattcttc acgctccagt
     2221 cccgccatgt tcagaatcag ccattcccgt gccgctcggg tggaggtgaa ggccacgccg
     2281 ggttcatgaa gatggcggtc cagggttttc agggcccgca gaatgagact gcgctcgccg
     2341 ggcgtcatct ctccgggcag aaaggaaagt tgttgcattg tgcttctctc cattcagtcg
     2401 atgatgcgca taatggcgct gcattccgga tgctgcaggg cgtaatcccg caaccggtaa
     2461 taatggaccg tcatggcata acactccgta cgacaggcat gatgactgta cgtcatcaga
     2521 caggcagcaa tgccggcggc ttccgggctc atttcagcgc ggttaccgtt catggcattg
     2581 aacagtaccc agttttcgcc atcatcgtca tccggttcgg gggccataaa tgcgccaccg
     2641 ttgttcagtg tgtacagatt ccagatacca ccgcagtagt cttcgcaaag acggtccatc
     2701 cagccgaaga tacggggctc cagggtcacc cactgtggaa tgaggccaaa atgctgcggc
     2761 cagaagctga tgcgctgttc atcagggact atggtggcaa ccagctgagg ctggtcactc
     2821 cctgatgcag ttgttacgga aacagaaggt gtggtggagc tatgcgaaaa ggttgtcatg
     2881 aaatgattcc ttataaaaag caaatgaatg aagaagactc gggggcaggg acagacgtga
     2941 gtcaggacgg cgctttcagg taaacggtat cagcgcatgc tctccagcag cgtttccgcc
     3001 atcacccaca atgcgcgatt gagcttgatg tcggtgtcga tactgtgaat ggcacgggta
     3061 tggatacgtt ttccttttgc actacggccg gaaatcccgc ctttcagcat attctcctgg
     3121 atggtctgat atgcgctcca caggtcctta ccgtaatcct cccggcgtcg tggtgtcaga
     3181 atgtcggcag tggtgacggg ttgatgttca tcaccataac ggtaagtcag cgccgcctgt
     3241 gccagtgcct gacgtgccgg tggcggcaga accagagact gcatggcatc acgcttttcc
     3301 tcaatccggt caaaaacgcc cactacctcg taagcccctt caatgactct gtccaccaca
     3361 tttccccggt ggggcacacg gacttccccc agagactgac cgcagacgca gccgttctgg
     3421 cagacgaatc tgaagtaacc cggcagcatc tggtagctgg aggtaccgtc atgggagttg
     3481 agcagaataa tttcaggcac atgctgaccg gttatctgtc cggcccgccg cagacgcagc
     3541 atgtgtttgg tatattcccg gcggcccggg tcacgtacgc gggtctggca ggcgaagaat
     3601 ggctgaaagc cttcccgctg caggctttcc agcacggtga tggtgggaat ccaggtatag
     3661 ttctcgcttc tggatgtgtg tttctcttcc ccgaatacgc tggggacgac ctgcatcagt
     3721 tcttcacgag ttaaggggcg gtcacggcgt atctggttcg cataaccaaa acgactggct
     3781 aatcgcatga tttattcctt atgaatgagt tagcggtata aatgaaaaag ccgcgtctcc
     3841 cggagaagac gcggcctgat gggggaaatg aatgacgttt attgtctgag aagcccttca
     3901 ctggcgagct gcgtattttt atggaaggag atcacccaat aaccccatcg ggatcgggta
     3961 ataaccggtc agactgtgat cgattcagat gccatttggc agcaaaagag ccttcggcaa
     4021 taaacgtgct gtacaacccg gcatatctgt tgctccctga aagtaaaaag ccccggtcat
     4081 gatgaccggg gcctgaaggg gagtgacctg attatcagaa agtcacattc agcgtggcct
     4141 gaccgttata gccttcagcg ctgctgccgc tgacgctgtg ggcataaccg gcctgaacgc
     4201 ccagggtgat attttcccgg acacgggctt ccagtccggc ctgcaggtcc agtgatgtgc
     4261 cattctgtga gggagagaac gtcatcccgc tgcctgcagt ggaagtcccc acacgcatat
     4321 ctccccggga gctgaaggtg cggataacgg aaggctgtac ccaccagttc accggcagtt
     4381 cacgcacact gtgttttgca ctgtcacgca ggggggcacg ggatgaggtg ccttcaccaa
     4441 aggtcatatc gttgtggctg cccagacgga aaccggcacg cacatgctgt gcactgccat
     4501 gcccgaactt cacataaccg gcgttgtcct taccgtcatc cagcgaaagc ccctgccagg
     4561 tgtactgcag ccgtggctcc agcatcaggt tgtcagtgat actgaagggc agaccggttt
     4621 ccagtgagcc cagccagccc cagccccgtg cgcggaagtc gttattgccc gatgacgctt
     4681 tcatactgtg gcgggttccc tgtgccacaa tgtcagccca caggccggag gaggtgtgtg
     4741 tcaggttcat atatccgccc aggctgccgg catcatcccg gaccgtgccg gcgcgggagc
     4801 cgtcatcatc cttaacatca acggaagaat ggcctgcagc accatacact cctgtcgtca
     4861 gtgacatacc ggcgacctct gttctgagca ggtcaccctc cag
//
LOCUS       DAZKJR010000239         4857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKJR010000239 DAZKJR010000000
VERSION     DAZKJR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.240"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            138..854
                     /gene="nanC"
                     /locus_tag="ACHOWS_004981"
     CDS             138..854
                     /gene="nanC"
                     /locus_tag="ACHOWS_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFQ0050424.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            874..1980
                     /gene="nanM"
                     /locus_tag="ACHOWS_004982"
     CDS             874..1980
                     /gene="nanM"
                     /locus_tag="ACHOWS_004982"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFQ0050425.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRVYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2045..3025
                     /gene="nanS"
                     /locus_tag="ACHOWS_004983"
     CDS             2045..3025
                     /gene="nanS"
                     /locus_tag="ACHOWS_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFQ0050426.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYSSHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            complement(3033..3683)
                     /locus_tag="ACHOWS_004984"
     CDS             complement(3033..3683)
                     /locus_tag="ACHOWS_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0050427.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEELKALMDANKSN"
     gene            3820..3963
                     /locus_tag="ACHOWS_004985"
                     /pseudo
     CDS             3820..3963
                     /locus_tag="ACHOWS_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4671..4754)
                     /locus_tag="ACHOWS_004986"
                     /pseudo
     CDS             complement(4671..4754)
                     /locus_tag="ACHOWS_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025205684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1415 a         1036 c         1136 g         1270 t
ORIGIN      
        1 cttatacaaa aaatataaaa taagctaccc gttgccaaag acactataag cctggcaaaa
       61 aatattacac aacataaatg ctaattgttt atgcgggctt tgtattgctt tctgtatcct
      121 acaaatgagt gaaatttatg aaaaaggcta aaatactttc tggcgtatta ttactgtgct
      181 tttcgtcacc attaatttct caggctgcga cactggacgt acgtggtgga tatcgtagcg
      241 gaagccatgc ctatgagact cgactcaaag tcagtgaggg atggcaaaat ggatggtggg
      301 caagcatgga aagtaatacc tggaatacca ttcatgataa taaaaaggaa aatgccgcac
      361 tcaatgatgt tcaggttgaa gttaattacg cgattaaact tgatgatcaa tggacggtgc
      421 gcccgggaat gttaacgcat tttagcagca acggcacacg ctacggaccc tacgtaaaac
      481 tgtcctggga cgcgacaaaa gatcttaatt ttggcattcg ctatcgttac gactggaaag
      541 cttaccgaca acaagactta tccggtgata tgtctcgtga taacgttcat cgttgggatg
      601 gatatgtcac ttaccatatt aatagtgatt tcaccttcgc atggcaaacg acgctataca
      661 gcaaacagaa tgattatcgc tatgcaaacc acaagaaatg ggcgacggaa aatgcctttg
      721 ttctacaata ccatatgacg cccgatatta cgccatacat agaatatgac taccttgacc
      781 gtcagggtgt ttacaacggc agagataatt tatcggaaaa cagttatcgc attggtgtgt
      841 catttaaact gtagtagaca ggagacagtc acaatgaata aaacaataac ggcgcttact
      901 atcataatgg cttcatttgc cgcaaacgcg tctgtattac cggaaactcc tgtgccattt
      961 aaaagtggta ccggagcaat tgataacgac actgtctaca ttggtttagg tagcgcaggt
     1021 acggcatggt acaagctgga tacacaggcc aaagataaaa aatggacagc gttagctgca
     1081 ttccctggcg gaccgagaga tcaagcaacc tctgcattta ttgatggcaa tctgtatgtg
     1141 tttggcggca ttggcaaaaa cagcgagggc ttgactcaag tatttaatga cgtacacaaa
     1201 tacaacccca aaaccaatag ctgggttaaa ttgatgtcgc acgcgccgat gggcatggcg
     1261 ggccatgtga cttttgtaca caacggcaag gcttatgtta ctggcggtgt taaccagaat
     1321 atcttcaatg gctattttga agatctcaac gaagctggaa aagattcaac cgctatagat
     1381 aaaatcaacg cccactattt tgacaaaaaa gcagaagatt atttcttcaa taagtttctg
     1441 ttgtcttttg atccctcaac acagcaatgg agttacgctg gcgaatctcc ctggtacgga
     1501 acggctggtg cggcggttgt gaataaaggt gataaaacct ggcttattaa tggcgaagcc
     1561 aaaccaggat tgcgaacgga tgccgtattt gaacttgact tcaccggtga taatttaaaa
     1621 tggaataagc ttgctcccgt cgcatcacca gatggcgtcg ctggcggttt tgcggggata
     1681 agcaatgatt ctcttatatt tgccggaggg gccggattca aaggttcgcg ggaaaattac
     1741 caaaacggta agaactatgc gcatgaaggc ctgaaaaaat catatagcac tgatattcat
     1801 ctttggcata acgggaaatg ggataaatct ggtgaattat cgcaaggtcg ggtctacgga
     1861 gtatcattgc cctggaataa tagtttatta attattggcg gtgaaactgc aggcggcaaa
     1921 gcggtgacgg attcagtttt aatttctgtg aaggataata aagtcacagt acaaaactaa
     1981 cgcttcaggg cctcggtaaa ggggccctgc tatcaatttg tcatttgtat tacgcgaaaa
     2041 ttatatgaac gcaataatat cgcccgatta ttactatgtt cttaccgttg ctggtcagtc
     2101 taatgccatg gcgtatggcg aaggactgcc attaccggac agggaagatg cgcctcatcc
     2161 cagaattaaa caattagcga gatttgcgca tacgcatccc ggaggcccgt catgtcactt
     2221 taacgacatt attccactga ctcactgccc acacgatgtt caggatatgc agggttatca
     2281 ccatcctctg gcaacaaatc atcaaacaca gtacggcacc gttggccagg cactgcatat
     2341 tgcacggaaa ttactgccct ttattcctga taacgcaggg gttctcatcg ttccgtgttg
     2401 ccgtggcgga tcggctttta ccgcgggcag cgaagggaca tattcagaac ggcacggagc
     2461 cagccatgat gcttgtcgtt ggggaacgga tactccgctg taccaggatt tagtcagcag
     2521 aacgcgagcc gcactggcaa aaaatccgca gaacaaattc ctcggcgtat gctggatgca
     2581 aggcgaattt gacttaatga cctgtgacta ctcgtcacac cctcaacact ttaatcatat
     2641 ggttgaagcc tttcgtaggg atctaaaaca ataccattct cagcttaata atattactga
     2701 cgcaccgtgg ttttgcggcg ataccacctg gtactggaaa gaaaatttcc ctcatgcgta
     2761 tgaagcgatt tatggcaatt atcaaaataa tgttttagcc aatattattt tcgtcgactt
     2821 ccagcaacaa ggtgaaagag gactgacgaa cgcgcctgat gaagatccgg acgatttaag
     2881 cacgggatat tacggttcag cgtaccggtc accggagaac tggacgacgg cactgcgaag
     2941 cagtcatttc agcgcggcag cccgtcgggg gattatttct gacaggtttg tagaagcaat
     3001 tttgcggttt tggcgcgaaa ggtgagcgtt cattaatttg atttatttgc atccatcaac
     3061 gcttttaact cctccggtga aaacggtggt ttgcgtcggt gcagcatcgc atggcaatta
     3121 gggcacacag gcagcaaatc attaactgga tccagctggt aatcctcttt aatggctgaa
     3181 agcggcacca ggtgatgcat atggataaag ccttcagcga tatcaccata gatcttcacc
     3241 agatcaacgc cgcatacgtt gcactggcat ccatgatgtt cgaccgcttt atctcgggcc
     3301 tttttatcgc gttcatagcg gtttactgtc acctttatcg ccgcaccttc aaggtatgtc
     3361 tcctgcgacg gcaactcatc cgggaacaga acgtcggcta tgggagccgc agcggcttta
     3421 tcctgatgaa tatgaaatcc ctgctctatg agtagtttga cgcatttaga ttttattccg
     3481 ccggtgaaat ccgcaggggt aaattgcgtc cccgtcatca atgtcgctgc gatgccagca
     3541 atcgccttcg aggggtaacg tcggccatga tattcaatct catacaaccg cgcaggctgg
     3601 aactgatgag tttctcccgc atcccataaa tggatagctt cgatgatata ccgccgcgga
     3661 agatgatcag ggagtttatt catggagaat gtccttatac gttctcagaa caggcagcaa
     3721 aaaaacaaat gagatcaagg taatatatct tcacttgcag gcttgaacag cagaaaattt
     3781 gcgagcatga tcgcaattat ttcactgcca gggatatcaa tggcttccag taaatcgcta
     3841 cagcaggcaa ttgccaatat aaaaatctgg cacaagggtg aacagcgcgc gccgcataag
     3901 ccattgttat tgctatacgt attagcggga tacctgaatg gacatccgcg ccttttcgat
     3961 tattgtagtg gaacagtcta cgaggatgca gttcgaagtg agtaagtaaa ggttgctgga
     4021 aacgatcaat gccgccatac aatgattttt gaggatcaac aatgagtaat aaaaactacg
     4081 aaagtcaccg caaagcgatt gttagtaaag gtataccacc cgctctatta aataggctca
     4141 ccaattcgga tgttcaggtg atcaatacct tcttaacacg agtgagcaaa ctggaactat
     4201 ctcaacaaga gaaagactgg atcataaaaa tcatctctat ggtttagaca atttcagcct
     4261 tgtagccttt caagggtttt gtgtgggtac aaacattatc gaatcgggat atagccgcag
     4321 ctgtagctgt atccacatca gtaagttgat gtaggttgac ttaggttgag cgaaaaatag
     4381 cgaaaagcct tgtgtggcgc ggatttcagt cataaaaaaa cgtccgttga cgtctattga
     4441 tgttccgatg gtgcgaaggt cgaaggccgg actcgccttt gcgtttaatt cgttgaaaaa
     4501 taatggtttg tgagatttta ttttgaggat ttgcccccaa aatgggacat aaaattgaat
     4561 actgggtgga agatcacaaa gttgcgtaat tattttccag gtccgaaacg cattcaagag
     4621 caaggcctct cccggcactg cgtcagtcgg gatgctaaat ctcccacatg tagccctagt
     4681 aaatcgcgcc agaatgtcag gctagtttgc aaattactga cggcgagcgt taagtggttc
     4741 agagattgaa gcataggatg cctattgatg gttaataatt taaccaaaca ctaatagcta
     4801 ataaaacccc acatggaaag agattttttg cgggtcttgt tttgtaataa atttctt
//
LOCUS       DAZKJR010000240         4846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKJR010000240 DAZKJR010000000
VERSION     DAZKJR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.241"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(690..1133)
                     /locus_tag="ACHOWS_004987"
     CDS             complement(690..1133)
                     /locus_tag="ACHOWS_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050428.1"
                     /translation="MHHYKFFALIAMLLCGASFASDVYFLCNTEKGTIKLDENKGVLR
                     YTLSKDHKTEFNYESKGSDYSGFKYNHYSRFQTDYFNVSFVNSEYKYTIFSNYEDDRK
                     SSGVSVTSLNSKKESVYYCKSIGIDRLSDLSAKMACDKYSALGCD"
     gene            complement(1120..3432)
                     /locus_tag="ACHOWS_004988"
     CDS             complement(1120..3432)
                     /locus_tag="ACHOWS_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HFQ0050429.1"
                     /translation="MMNIRYPVRKADGRDYKSYDELLTDIRKNAHGWWLLGVNRYWHG
                     GIHVGRSSSPASVLNQETPEKSVPLQFMMDGEVVAWRVNRDYAAIECYQERPLRQSGT
                     FVLVKSVYKPDEQDESSWLTLYQLYMHIAPLSEFPKRPLYRVTQKGHGVRMRKYSRHD
                     DSREIAPDVLANKHGHPRTLTQGDTLAVLQQKSFLLKQRPESFALVQRLQDGKPAGEL
                     FWVSMRPEYLEPDGQCYVCLPDWMHSALNHGVFDDVVVPPVPLKVMVKAGDAVGFLGA
                     QDLADEDNYPQIITTDYKAHIELLSLDEHVPDVVANMKGIKTGKQFIKLKLKRPLYLR
                     NGEGEESTFEQMSAITRIDAGKIIPRDATYPFTDKTGITYFQIRPHTWMHQNDVEQLS
                     QHDLAGLNFHCIEAEHTTDFTRTLDERWLIDALKSISSHFDGEKGPQFAQAKMFYDSL
                     IHNAENRRAPNPYPDKSLDQYLFGALHTNQMNIPEYARRLIVKHDSCWHSTRDDTRWS
                     SVCKSRDESPVVKMANGGFLDATHWMDKVPPFASQRSVWHFHPLEFPEILRSAEFPRT
                     PINGVLTPIEFIHFYNGDKIDDTDYEEAAKELGCEVAAIKAVAKTETGSYGSYFKFED
                     NDDYVPAILFERHHFHKYTNGKYDQFEDISNPVAGGYGATSIQYAKLVKAYTLDKKAA
                     LKSASWGKFQILASNYATAGYASPEDFVFALSKSEKNQLKAFVSFIKADRVLLRSIRT
                     KDWLSFAQRYNGPRQKGYDLKMERNYNASL"
     gene            complement(3773..3987)
                     /locus_tag="ACHOWS_004989"
                     /pseudo
     CDS             complement(3773..3987)
                     /locus_tag="ACHOWS_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3993..4679)
                     /locus_tag="ACHOWS_004990"
     CDS             complement(3993..4679)
                     /locus_tag="ACHOWS_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFQ0050430.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQLGNVKALFESKPFITLSEPACGAGSMILAMVDTLNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
BASE COUNT         1290 a         1184 c         1004 g         1368 t
ORIGIN      
        1 atccaaacag ccccatactt caggttaaaa agaacaataa aaaatgatgc taaaattgca
       61 acattctcta aataaattca attaattatc taaacaaggt gaaactttca gaggaaatga
      121 gcgcggcccc ccctaataat cttaagctgg gtacagggaa gtatcgacca ttacgcagtg
      181 tcgatatcga atcgtacaaa aaacagtctt ggcgtgggat atttacacat aattattagc
      241 tcacaatctc cctatacctg tcgtgttttc tggggattcc tcagggagtg accatagatt
      301 ccgttttcca ggtgcccccc tttttctcca aaaattgaaa aataactctg tagatgccgc
      361 taacaatcaa ttcgagttga tattttaaag cgttctgtgc agaaagaaat aaatttcaaa
      421 aatgaatcag gggagaaaag tgcgcttttt aacctgttca gtgtccagaa cacatgaaac
      481 cgatcgccat tcagtttcat gcatagtcat gcaacgcact gaaaatgatt ctgacgcatt
      541 tttccggttt cccccgagga aaacatccct ttttgcgtca gaatcgcgct cagcgcgttt
      601 tagcggcacg tacaattaca ggattataaa tcgtcaccag cgactggcgt aaaaaagccc
      661 ctctctgcag gcgagattta gatgttttat taatcacacc ccaaagctga atatttatca
      721 caagccattt ttgccgacaa atcactaagg cgatcaatgc caatggactt acaataataa
      781 acagattctt ttttgctatt caggctcgta acactaactc cgctgctttt tctatcatcc
      841 tcataattac taaatatcgt gtacttatac tctgagttta caaacgatac attgaaataa
      901 tcggtttgaa atcgtgagta atggttgtat ttaaaccctg aataatcact acctttcgat
      961 tcataattaa attcagtttt gtggtctttc gataaggtgt accgaagtac acctttgttt
     1021 tcatctagtt tgattgttcc tttttccgtg ttgcataaaa aatacacatc acttgcaaac
     1081 gatgccccac acagaagcat tgcaatcaac gcaaagaact tataatgatg cattgtaatt
     1141 tctctccatt ttcaaatcat atcctttctg ccttggtccg ttatatcttt gagcaaatga
     1201 aagccaatct ttagtccgaa tagagcgcag taatactcta tcagccttaa tgaagctaac
     1261 gaatgctttt aactgatttt tctcagactt acttaatgca aaaacgaaat cctcagggct
     1321 cgcgtagcct gctgtggcgt agttcgacgc taaaatctga aacttacccc aggatgctga
     1381 ttttaaagct gctttcttgt ctaaagtata cgctttaact agctttgcat actgtattga
     1441 tgttgcacca tacccacccg caacagggtt tgatatatct tcaaactgat catacttccc
     1501 atttgtatat ttatgaaaat gatgcctttc aaataatatc gctggaacat aatcatcgtt
     1561 atcttcaaat ttaaaatagg agccataaga accagtttct gtttttgcta cagctttaat
     1621 tgctgcaact tcgcatccta attcttttgc tgcctcttca taatcagtat catcaatttt
     1681 gtctccatta tagaagtgaa taaattctat aggagttaat actccattaa taggagttct
     1741 gggaaactct gcacttcgta atatttccgg aaactcaagc ggatgaaaat gccatacaga
     1801 ccgctgactg gcgaacggag gcaccttatc catccagtgt gttgcatcca gaaatccccc
     1861 gttagccatc ttcacaaccg ggctttcatc cctggactta cataccgatg accagcgtgt
     1921 atcatcgcgg gtactgtgcc agcagctgtc gtgtttcact atcaggcgtc gcgcatattc
     1981 cgggatgttc atctgattgg tatgcagcgc accaaacaaa tactggtcca gagatttatc
     2041 gggatacggg tttggcgctc ttctgttttc agcgttgtgg atcaggctgt cataaaacat
     2101 ttttgcctgc gcgaactgcg gtcctttttc accgtcaaag tgcgagctga tgcttttcag
     2161 ggcatcaatg agccagcgtt catccagcgt gcgggtaaaa tctgttgtgt gttcagcttc
     2221 aatacagtga aaatttagcc ctgctaaatc gtgctgactg agctgctcca catcgttctg
     2281 gtgcatccac gtatgtgggc gaatctgaaa gtatgtgatc ccggttttat ccgtaaacgg
     2341 gtacgtggca tcacgtggga taatttttcc ggcatcaatt cgggttatgg cgctcatttg
     2401 ttcaaatgta ctttcttcac cctctccatt tcgcaggtac agcgggcgtt tcagcttcag
     2461 cttaatgaac tgtttgcctg ttttaatccc tttcatgttt gccaccacat ctggcacatg
     2521 ctcatcgaga ctcagtagct cgatatgggc tttatagtcc gtggtaataa tttgcggata
     2581 gttatcttcg tcagccagat cctgagcgcc gaggaatccg accgcatccc ctgctttcac
     2641 catcactttc agtggcacag gcggcaccac cacatcatca aacacgccgt gattcagcgc
     2701 actgtgcatc cagtcaggca ggcacacgta acattggcca tcaggttcca gatattccgg
     2761 acgcatcgag acccagaaaa gttctccggc gggtttaccg tcctgaagac gttgcaccag
     2821 agcaaacgac tccggccgct gtttcagtaa aaacgatttt tgttgcagta cggcaagcgt
     2881 gtcaccctgc gtcagtgttc tggggtggcc gtgtttgttt gccagtacgt ccggcgcgat
     2941 ttcacggctg tcatcatggc gggaatattt acgcatccgc acaccgtggc ctttttgtgt
     3001 aacccggtaa agcgggcgtt tcggaaattc tgacaatggc gcgatgtgca tgtaaagctg
     3061 gtacagcgtc agccaggatg attcatcctg ctcgtctggc ttgtacactg attttaccag
     3121 cacaaaggta ccggactggc gcagggggcg ttcctgataa cattcgatag ccgcgtaatc
     3181 gcggttaacc cgccatgcca ccacttcgcc atccatcatg aactggagcg gcaccgattt
     3241 ttcaggtgtt tcctgattca gcaccgaggc cggggacgat gacctcccga catgaatacc
     3301 tccgtgccag taacgattta cgccaagtag ccaccaaccg tgggcgtttt tgcggatgtc
     3361 ggttagcagc tcgtcatagc tcttatagtc tcgtccatcg gctttccgta ccggataacg
     3421 tatgttcatc atcttttctt aagtgaaatc acatatttcg atggattctc gcgcgtattt
     3481 tgggtttggg gtgtagtgga accaaaaaga cggttaagcg gttaaattga atggtcaata
     3541 acaggtaaat caatctgttg agatcaacga cgtcttttgc tccgcaaacg gctaagatga
     3601 gtacagataa tttggttatc gtaaaaaagg tggctaaaaa tttcaacctg caatgtaaat
     3661 tggccggttg atttttggct atttaaattg acctcttaac ggcgtttatt gttccattgc
     3721 tccccaaccc cctatggatt ataagccgca catacattac ttcgttctgc atatcaccac
     3781 taatctgctt aagatgtact aacaacatca tcgcctgttg gctaaacgga actataactc
     3841 ctctttcatt ttcatgccgc gatacaagta atgcacacct ttaatttctt cacgttttgc
     3901 cggaatgcgc caaatagcgt tatcaaaatc aaattctttt taacgagcaa aacgccagtc
     3961 cgaagactgg cgcagtgata aaacattctg catcatgcca cattctgctc ctgaagatga
     4021 cgcagacgat ggtcccagtt tcccaggtaa tgcccgggag tcagcagtac gcggcgtcgc
     4081 tcattacaga gcgagttacc aatcaccact tctccggcca caccacacag cgacagttgt
     4141 atatacgcca tgccggctgc cagcgggtca atatccgttg ccgacaccca catcctgtgg
     4201 tatgccggat accccgaacg gttcagggtg tcaaccattg ccagaatcat actgccggca
     4261 ccacaggcag gttcactgag cgtgataaag ggtttactct caaacaacgc cttcacattt
     4321 cccagttgca tctgtgccat catgctggca acactccagg gcgtgaaaaa ctgactccgg
     4381 tatttatccc ccagttccag ttgcatgaac acactaccca gaaaatcaca gaatccctgc
     4441 tccagtccct caatgacatg agcaagcaaa tgcgcaatgt ggataacatc ctgcttttca
     4501 taaccactga cgattcgcag atacttctgt tcccgggcct cactgaactg aagccggttt
     4561 tccagggcaa tcacactaca gctgacaaag tcttcaaaca ccttatgacg tttgtgataa
     4621 caggctgtcc ggttaaacaa cgaaatgaat tccttctgat ggtcagtcat ggtggccatg
     4681 ttttctccgt aaaaacaggg aacacatggc ccggacaggg gaacaggtgt tccccttcgg
     4741 gttgaaatgt tgataaaacg ggcatcacgg acataatacc cggaggttaa ttactgggct
     4801 gagaccagag gaaccggtaa aggtgcggct ttcgtctgtt cttcag
//
LOCUS       DAZKJR010000241         4669 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKJR010000241 DAZKJR010000000
VERSION     DAZKJR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.242"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..1114
                     /gene="pstS"
                     /locus_tag="ACHOWS_004991"
     CDS             74..1114
                     /gene="pstS"
                     /locus_tag="ACHOWS_004991"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0050431.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1201..2160
                     /gene="pstC"
                     /locus_tag="ACHOWS_004992"
     CDS             1201..2160
                     /gene="pstC"
                     /locus_tag="ACHOWS_004992"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFQ0050432.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALTVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2160..3050
                     /gene="pstA"
                     /locus_tag="ACHOWS_004993"
     CDS             2160..3050
                     /gene="pstA"
                     /locus_tag="ACHOWS_004993"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0050433.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3141..3914
                     /gene="pstB"
                     /locus_tag="ACHOWS_004994"
     CDS             3141..3914
                     /gene="pstB"
                     /locus_tag="ACHOWS_004994"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0050434.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            3929..4654
                     /gene="phoU"
                     /locus_tag="ACHOWS_004995"
     CDS             3929..4654
                     /gene="phoU"
                     /locus_tag="ACHOWS_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0050435.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
BASE COUNT         1071 a         1191 c         1299 g         1108 t
ORIGIN      
        1 ctgtcacctg tttgtcctat tttgcttctc gtagccaaca aacaatgctt tatgaatcct
       61 cccaggagac attatgaaag ttatgcgtac caccgtcgca actgttgtcg ccgcgacctt
      121 atcgatgagt gctttctctg tgtttgcaga agcaagcctg acaggtgcag gtgcaacctt
      181 ccctgcgccg gtgtatgcca aatgggctga cacttaccag aaagaaaccg gtaataaagt
      241 taactaccag ggtatcggtt cttccggtgg cgtaaaacag attatcgcta ataccgttga
      301 tttcggtgcc tctgacgcgc cgctgtctga cgaaaaactg gctcaggaag gcctgttcca
      361 gttcccgacc gtgattggcg gcgtggtgct ggcggttaac attccagggc tgaagtctgg
      421 cgaactggtg ctggatggta aaaccctcgg cgacatctac ctgggcaaaa tcaagaagtg
      481 ggatgatgaa gccatcgcca aactgaatcc gggtctgaaa ctgccttcac agaacattgc
      541 tgtagtacgc cgcgcagatg gctccgggac ttccttcgtc ttcaccagct acctggcgaa
      601 agtgaacgaa gagtggaaaa acaacgttgg tactggctct accgtaaaat ggccgatcgg
      661 tctgggcggt aaaggtaacg acggtatcgc cgcgttcgtt cagcgtctgc cgggtgcaat
      721 tggttacgtt gaatatgctt acgcgaagca gaacaacctg gcgtacacca aactgatctc
      781 cgctgatggc aaaccggtga gtccgaccga agaaaacttc gctaatgcag caaaaggcgc
      841 agactggagc aaaacattcg ctcaggatct gaccaaccag aaaggcgaag atgcatggcc
      901 tattacctct accacgttca ttctgatcca taaagatcag aagaaaccag aacaaggcac
      961 cgaagtgctg aagttcttcg actgggcata caaaaccggg gctaaacagg cgaacgacct
     1021 ggattatgcc agcctgccgg atagcgtagt tgaacaggtt cgcgctgcgt ggaagaccaa
     1081 tattaaagac agtagcggca agccgctgta ctaataaaac tccaggccgg gtacggtgtt
     1141 ttacgccgca tccggcatta caaaatgact ttgtaaacgc gtttaactga agagtaactt
     1201 atggctgcaa ccaagcctgc ttttaaccca ccgggtaaaa agggcgacat aattttcagc
     1261 gtgctggtaa aactggcggc gctgactgtg ctattgatgt tgggtggcat tattgtctct
     1321 ctgatcatct catcctggcc gagcattcag aaatttggtc tggctttcct gtggaccaaa
     1381 gagtgggatg cgccgaacga tatctacggg gcgctggtgc cgatctacgg tacgctggtg
     1441 acctcgttta tcgcgctgct gatcgccgtc ccggtgagtt ttggtatcgc cctgttcctg
     1501 accgagcttg cgcctggctg gctgaaacgc ccgctgggta tcgccattga gctgctggct
     1561 gccattccaa gtatcgttta cggcatgtgg ggcttgttta tcttcgcccc gctgttcgcc
     1621 gtttacttcc aggagccggt cggcaatatc atgtcgaata tcccgattgt cggcgcgctg
     1681 ttctccggcc cggcctttgg tatcggtatc ctcgcggcag gcgtgatcct cgccatcatg
     1741 attattccgt acattgcggc ggtaatgcgc gatgtattcg aacaaacccc ggtgatgatg
     1801 aaagagtcgg cctacggtat tggctgcacc acctgggaag ttatctggcg tatcgttctt
     1861 ccgttcacca aaaatggtgt tatcggcggc atcatgttgg ggctggggcg cgcgctcggt
     1921 gaaaccatgg cggtgacctt tatcatcggt aacacctacc aactcgacag cgcctcgctg
     1981 tatatgccgg gcaacagtat cacctctgcg ctggccaacg aatttgcgga agcagagtcg
     2041 ggtctgcatg ttgctgcact gatggaactg ggcctgatcc tgtttgtgat taccttcatc
     2101 gtcctcgccg catcgaagtt tatgattatg cgcctggcta agaatgaggg ggcacgctaa
     2161 tggctatggt tgaaatgcaa accactgcgg cgctggctga atcccgccgt aaaatgcagg
     2221 cgcgtcgccg ccttaagaac cgtattgcgc tgacgctctc gatggcgacg atggccttcg
     2281 gtctgttctg gctgatctgg attttaatgt ccaccatcac tcgcggtatc gatggtatgt
     2341 cgctggcgct gttcaccgaa atgacgccgc cgccaaatac ggaaggcggt ggtctggcga
     2401 acgccctggc gggtagcggc ctgttaattt tgtgggccac ggtattcggt acgccgctgg
     2461 gcattatggc ggggatttat ctggctgaat atggtcgtaa atcctggctg gcagaagtga
     2521 ttcgcttcat taacgacatt ttgctctccg caccgtcgat tgtggttggt ctttttgttt
     2581 acaccatcgt ggtggcgcag atggagcact tctccggctg ggcgggcgtg attgcgctgg
     2641 cgctgttgca ggtgccgatt gttatccgca ccaccgagaa catgctgaaa ctggtgccgg
     2701 acagcctgcg tgaagcggct tatgcgctgg gtacgccgaa gtggaagatg attgccgcga
     2761 tcacgctgaa agcgtcggtg tccgggatca tgaccggtat cctgctggcg attgcccgta
     2821 ttgctggtga aaccgcgccg ctgctgttta ctgcgctctc caaccagttc tggagcacgg
     2881 acatgatgca gccgatcgcc aacctgccgg tgacgatctt taagtttgcg atgagcccgt
     2941 ttgcggaatg gcagcaactg gcctgggccg gggtgttgat cattaccctg tgcgtactgc
     3001 tgctgaacat tctggcgcgc gttgtttttg cgaagaataa acacggttaa cactttgctg
     3061 gatgcggcgt aaacgcctta tccggcctac ggtaagcctg attcgcgaag tgcatcaggc
     3121 acgatgagga aaagattgca atgagtatgg ttgaaactgc cccgagtaaa attcaggttc
     3181 gtaatttgaa cttctactac ggcaaattcc atgccctgaa aaacatcaac ctggatatcg
     3241 ccaaaaacca ggttacggcg tttatcgggc cgtccggctg cggtaaatca acgctgctgc
     3301 gtaccttcaa caaaatgttt gaactgtacc cggaacagcg tgcggaaggt gaaattctgc
     3361 tcgatggcga caacatcctg accaactctc aggatatcgc cctgctgcgt gcgaaagtgg
     3421 gcatggtgtt ccagaaaccg acgccgttcc cgatgtccat ctacgacaac atcgcttttg
     3481 gcgtgcgtct gtttgagaag ctctcccgtg ctgacatgga cgagcgcgtg cagtgggcat
     3541 tgaccaaagc cgcattgtgg aacgaaacca aagataaatt gcaccagagc ggttactctc
     3601 tctctggtgg tcagcaacag cgtttgtgta ttgcgcgtgg tatcgccatt cgcccggaag
     3661 tgctgctgct cgacgaacca tgttcggcgc tcgaccccat ctccaccggg cgtattgaag
     3721 agctgattac cgaactgaag caggattaca ccgtggtgat cgtcacccac aacatgcagc
     3781 aggctgcgcg ttgttccgac cacacggcgt ttatgtacct gggcgaattg attgagttca
     3841 gcaacacgga cgatctgttc accaagccag cgaagaaaca aacagaagac tacatcaccg
     3901 gtcgttacgg ttgattcagg agtgcgttat ggacagtctc aatcttaata aacatatttc
     3961 cggccagttc aacgccgaac tggaaagcat ccgcacgcag gtgatgacca tgggcggcat
     4021 ggtggagcag cagctttctg atgcgatcac tgcaatgcac aaccaggaca gcgatctggc
     4081 gaagcgcgtc atcgaaggcg acaagaacgt caacatgatg gaagtggcga tcgatgaagc
     4141 ctgcgtgcgc attattgcca aacgccagcc gacggcgagc gacctgcgtc tggttatggt
     4201 gatcagtaaa accattgccg agctggagcg tattggcgac gtggcggaca aaatctgccg
     4261 tactgcactg gagaaattct cccagcagca tcagccattg ctggtaagtc tggagtcgct
     4321 gggccgtcat accatccaga tgctgcacga cgtgctggac gcgttcgcgc ggatggacat
     4381 tgatgaagcg gtacgtattt atcgtgaaga taaaaaagtc gatcaggagt acgaaggtat
     4441 tgttcgtcag ctgatgacct acatgatgga agattcgcgc accattccaa gcgtacttac
     4501 cgcgctgttt tgtgcgcgtt caatcgaacg tatcggcgac cgctgccaga atatttgtga
     4561 gtttatcttc tactacgtga aggggcagga tttccgtcac gtcggtggtg acgagctcga
     4621 taaactgctg gcagaaaaag atagcgataa ataattcacc aagtaaatc
//
LOCUS       DAZKJR010000242         4632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKJR010000242 DAZKJR010000000
VERSION     DAZKJR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.243"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1230)
                     /locus_tag="ACHOWS_004996"
     CDS             complement(<1..1230)
                     /locus_tag="ACHOWS_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050436.1"
                     /translation="MVNQTMQQNQYYRILSGESRPFPGIYEAVSEQLLLLIFKARQLY
                     QDPEYRPWFEEQHKVLLALRDNTELQKIFNKQRKENFALGASTSSQPTALTNALNVMC
                     HLYNGFDEGNISLKSWGAMQEVQTTPDFFSLGPRTWKTPELAKFVGDDLFNILTECKA
                     WSSPIPAEARQGYCHQDLSVAHSCTSELLAHLKQYHEIDESTILAITIGLAVNEFYQY
                     KQNIPEWLSCFENKNKWPWDCRKSDTEDVHRLRERLSIIGCTEFVKNMFSVASQHEIK
                     TMYNIYVHIYAAEICGWVKTSMAYFKPTDFIQYTWKFATVTPDKDEKTENTTAWERDY
                     CQITIQRDELLRLIRTNTLQDAAIHLSGCLQYAYLESVKNDIDYPGIAPNAEEKKMKY
                     TDSIEFSGIRNNIINAVK"
     gene            1960..2187
                     /locus_tag="ACHOWS_004997"
     CDS             1960..2187
                     /locus_tag="ACHOWS_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0050437.1"
                     /translation="MWVPLTDEQRSVILKAYGTEGDRLVREKERKEITSISRSQAWKL
                     EREGNYPPRKTIGKKSCGWLLSDLLWWIQTR"
     gene            2283..3716
                     /locus_tag="ACHOWS_004998"
     CDS             2283..3716
                     /locus_tag="ACHOWS_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFQ0050438.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQQNTQAPLAMVATSALTA
                     MSIACQNQIDVCRPGNLRGPVNIYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNNSPVPPQIRRTI
                     FNDTTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTMSAMIQPDVYHKGFCTRKKELVKTSGHHARFLMCQPTSMQGTRIITG
                     DNYSSQYQELFDKRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYMRLSNPL
                     TFDNTALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT         1289 a          856 c          999 g         1488 t
ORIGIN      
        1 ttttactgca tttatgatgt tattcctaat tccagaaaat tcaatagaat ctgtgtattt
       61 cattttcttt tcttcagcat taggggctat tccaggatag tcaatatcat tctttacaga
      121 ctccagatat gcatattgca ggcagcctga aagatgaatt gctgcatcct gcagagtatt
      181 agtgcgaatc aatctaagga gttcatctcg ttgtatggtt atctgacaat aatccctttc
      241 ccatgcggtc gtattctcgg ttttttcatc tttatccgga gtaactgttg cgaatttcca
      301 tgtgtactgg atgaaatctg ttggtttaaa atatgccatg cttgttttta cccaaccgca
      361 gatttcagct gcataaatat ggacataaat attgtacatt gtctttattt catgctgact
      421 ggcgactgag aacatatttt ttacgaattc tgtacatccg attatcgata gtcgttctct
      481 cagtctgtgc acatcttcag tatctgactt cctgcagtcc cagggccatt tgttcttatt
      541 ctcaaaacag gaaagccatt ctggaatatt ttgcttatac tggtagaatt cgttgaccgc
      601 cagaccaatg gttatggcaa gtattgttga ttcatctatt tcatgatact gcttcaggtg
      661 tgccagcaac tctgaggtgc aggagtgcgc aacagacaga tcctgatggc aatacccctg
      721 ccgggcttct gctggaattg gtgaactcca ggctttacat tcagtcagaa tattaaataa
      781 atcatctcct acaaactttg ccagttcagg tgttttccag gttcttggtc ccaggctgaa
      841 aaagtctggt gttgtctgta cctcttgcat cgctccccag cttttcaggc tgatatttcc
      901 ttcatcaaag ccattgtata aatggcacat aacattaagc gcattagtaa gagcagtggg
      961 ttgagacgat gtagatgcgc caagagcaaa attttctttt ctttgtttgt taaaaatttt
     1021 ctggagctct gtattatcac gcaatgccaa gagaactttg tgctgttctt caaaccaggg
     1081 gcgatattct ggatcctgat atagctgacg tgccttaaaa atgagcaaaa ggagttgttc
     1141 ggaaacagcc tcatatatgc cagggaatgg gcgggattct ccggataaaa tacgatagta
     1201 ctgattttgt tgcatagtct gatttaccat tatgtaagaa ggtttccttt tgccgcatac
     1261 cggatactgg cagttaaaat cgatgttatc tcctgaaagg atacgttgat actgagtctg
     1321 catctactaa taaaacggat gcctgccgtg ttttcggggt gatacagggt atacctgaga
     1381 atgcatctat tattacgata attttcctcc agcgtgttgt aacaattata acacccgtca
     1441 gatctccttc ttcttcctcc tcctgaatat tccgatctgt tgaagctgtt gttgtcattt
     1501 caatgacagg taaaatgcct ggctctcttt ccctcctatt gtgaatagta aaaactaatt
     1561 tatttttgtg atcttggtca caaatttaac cccctccttc tcttctttac tatcactgtc
     1621 agaagtttat ctgagcataa catgaagtcg atactagatt cattttgtgt cattgtcaac
     1681 agtgaaaacc acttcttggc ttaaaatcat tgtttgtaat gtgcattatt ggttgttttg
     1741 ttgctaatca acacatattg acctatgagt gggcttgtga gtcaagtatt gttttatttg
     1801 tcttaacttg acttgatttg ttttcgtatg taccttgctg tccatgggtg ttctgtgagc
     1861 aatatgacaa cggaatctcc actatggatt gtccaggaaa ttcagtcatt tcatgctcac
     1921 aactgagata agaatcattg atatatcagg agggccgtca tgtgggttcc attaacagat
     1981 gagcaacgtt cagtaatttt gaaagcctat gggactgagg gtgacagact ggtacgtgaa
     2041 aaggaacgaa aagagatcac ctcaatttcc cgttcacaag cctggaaact tgaacgagag
     2101 ggaaactacc caccgcgtaa gactatcgga aagaaatctt gtggctggtt actaagtgac
     2161 cttctctggt ggattcagac cagatgaact gtttgtattt tcagtctgaa tagcagctat
     2221 tttcgctatt gtagcggaaa ttgctgcaac tttactaacc cattaatgaa aatggaggct
     2281 ttatgtgctt actagcacca gaaaatcctt atccgatata tgcactacca ccgctggtga
     2341 gaaatgcaat aattgaaact caacaaaata cacaggctcc tttggctatg gtggcgacat
     2401 ccgcattaac agcgatgtca attgcctgtc agaatcagat tgacgtgtgc agacctggaa
     2461 atttacgtgg gcccgttaat atttactctc tgattctggc tgattccggt gaaaggaaaa
     2521 cgaccgtgga taaagtgttt atgaaagcat tttatctcag ggatgaagcc ctggcggaag
     2581 aatacgcgaa actggttgag aattacagta cagaaaagga aatatgggag caaaaacaaa
     2641 aagcgctgga atcaaaattt cataaagaga ttcgtgccgg taaagattac aaggcaacag
     2701 aatcagagct tgaaacgcat ctgaataact ctcctgttcc gccgcagata cgtcgaacaa
     2761 tttttaatga tacaacgata gagggaatgt taaaatatta ctccgatagc aatcgttctt
     2821 ttgctcttgt atccagtgaa gggggagtaa tttttgacag ccgggccatg agtaaactgg
     2881 gaattattaa cactttgtgg gatggaggtt ctcttttcat cgacaggaaa tcatctcccg
     2941 gaattaattt gaaggagcca agactgacga tgtcggcgat gattcagcct gatgtttacc
     3001 acaaaggttt ttgtacgcga aaaaaagaac ttgtgaaaac gtcaggacat catgcaaggt
     3061 ttttgatgtg tcaaccaaca tcaatgcagg ggacaaggat aataaccgga gataattatt
     3121 catcacagta tcaggagtta tttgataaga ggatcaatga acttattgat gaaagtctgg
     3181 cgatgagtgg tgaacgacgt tgccttcact tttctcctca ggctgccaga atctggacgg
     3241 attattacaa tgatgtggaa tcgaaactgg ggggattggg gcctttaaga cattgtcggg
     3301 aatatgctgc caaaaatgca gagtatatgg caagactggc aggacttatt taccattcga
     3361 gcggtgaaga gggggagatt tccccttaca ctgcagaaat ggcgagagaa ttagcaatat
     3421 ggtacggtaa tgagtatatg cggttgtcta atccgttaac ttttgacaac actgctctga
     3481 ccgtacctgt gcgactcatt ccagaggagc ttgaactttt caactggata aaaagctatt
     3541 gcattgagaa ggggatcctc tgtatgaaaa aaaatgacat tttacagcgt ggtccgaacc
     3601 gtttccggaa aaaggataaa atcaactggt tactggattt actgtatgaa caaaacagag
     3661 ttgtaccggt tattgaggga aaaacgttgt gtgtcgcacc taactttgac ctctgaacga
     3721 cttaatcgtt gagtaacata tagtctcctc cgttacttgc ggaggagatt aatgccatga
     3781 ccatgtcagt gttgtattac agaactttgg tacaggtcag tcagtaaata taactggtgt
     3841 aactactatt tactattgga tagagtgttg tacaggatat tgctggcttt ggccttagat
     3901 aaggagggca cttcattgtt ttcgtattca ataaataatt taggttaatt agtattatta
     3961 atgggagtta tgttatctgt tatgttgtct ctttctatcg taatgtcact cctctttatg
     4021 ctgagtattc tgtatgtgcg taatgcgtct atccccatat gctacagtgt ggtggtgtat
     4081 ggggattagt tatgtgtaaa ttatgtcact cactcatagc gtaatattga gaaagttaat
     4141 ccattgagtg tgtgtctggt tcgtatttaa cactagttac gtaccagaac actttaggga
     4201 tatattatct ttggttcgta cgttatagta gcgttacagt ttataagtat gggtaaatat
     4261 aatcttttct gtatgaaata atcttcttat ggtgtgtggg gtatcaatag cggtgttgcc
     4321 tgtagctacc cgatactgac ttgtgtgaat gtcagattac ttcctgtaag tgattgaatg
     4381 gtacttcctt aaacttaatt ctatgaggtg tgtttttacc attgtaattc gtactggtcc
     4441 tcagtttaag tgagtctcac tgtattctga agtattacct cattgctgta atgaggtaat
     4501 acaaaacagt tttaacaggt tgtctgtact gctggttatc tcttttatta atgccgtggt
     4561 acgacgtttc tgtaccctca gcatttacat aatttcctct tcctgtgagt tttataaagt
     4621 aaggactata aa
//
LOCUS       DAZKJR010000243         4624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKJR010000243 DAZKJR010000000
VERSION     DAZKJR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.244"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..531)
                     /locus_tag="ACHOWS_004999"
     CDS             complement(<1..531)
                     /locus_tag="ACHOWS_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0050439.1"
                     /translation="MNKYIIPWCFIVFIFSFSGATFAAPKGICTSDNGSFHSTLDFSG
                     YLITADENRVGKTFNKTVTNGDSYPAHCHCDTGNVGEFPYIYYTARINESLGYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIIPE"
     gene            complement(542..3064)
                     /gene="lpfC-O113"
                     /locus_tag="ACHOWS_005000"
     CDS             complement(542..3064)
                     /gene="lpfC-O113"
                     /locus_tag="ACHOWS_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFQ0050440.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQVQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKELGALGAISFDVTHARAKFSHADTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDQGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            complement(3089..3820)
                     /gene="lpfB-O113"
                     /locus_tag="ACHOWS_005001"
     CDS             complement(3089..3820)
                     /gene="lpfB-O113"
                     /locus_tag="ACHOWS_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFQ0050441.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGT
                     RVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
                     IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(3868..4440)
                     /gene="lpfA-O113"
                     /locus_tag="ACHOWS_005002"
     CDS             complement(3868..4440)
                     /gene="lpfA-O113"
                     /locus_tag="ACHOWS_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFQ0050442.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         1277 a         1096 c          983 g         1268 t
ORIGIN      
        1 ttcaggaata attaactttc cagcaaaagt cttcttaata taaaaataaa ttattgcttt
       61 tgcaccactg gaaatactta atgggtccgt gcgactacat ttatatgaac ctccagtagg
      121 cctgttagca tggtattcaa aaggtgcatt aatgtatcct acaccgagaa ggtctatcga
      181 tattccaaca tccagattag gattcaaatt aaagtagttt acattagaac gaacacctgc
      241 ataacctaag gactcgttta ttctcgctgt ataataaata taaggaaact ctcccacgtt
      301 ccctgtatca caatggcagt gagcaggata agagtcccca tttgtcacgg ttttattaaa
      361 ggtctttccc actctgttct cgtctgccgt aatcagatag ccggaaaaat caagtgtgct
      421 atgaaaggac ccattatctg aagtgcagat accttttggg gccgcaaatg ttgcgccact
      481 aaaggagaag ataaaaacaa taaagcacca tggtataata tacttgttca taaaaatcct
      541 tttaatgaca tgtcacacct tgttcaatta agccgctttc actttctggt attgaaagtg
      601 aataatcgaa ggtgcatgta gtggcggcat tttctcccca ggagagcatc acctgccctt
      661 tttcaggaag gcccgagaga taaagctccc cctggtcacc gacaattcct gaatcagcgg
      721 agcttgtatt ttgtactgta gcaatagcgc caaaaggtgc tggtttacct tttgtacccg
      781 caaccctgaa aagcacacga taaccaacac gagtaacaaa ttcggcctta accactgcac
      841 cacgggtggg aaccatcgta acgacggtat tatcaagttc tatattcgac ttcgagaaat
      901 agttatcgct gattgctacc cggttttcat cataagggtt tagctgtgtc ttgaccgtat
      961 agccgcgcca gtccgtcgca gcaccgcgca tattgtctat ttctaacccg gcagcccctg
     1021 gtgctttaac aagagctatg gtttcaccca actcctggct taacgttacg ccttcgctgt
     1081 gcgccagaat tccccctctg ataccataat tcagcctctg agaattttgg ctgtagttat
     1141 agctgccgtt caccgtgcca taagtgccat cataggtcag ggacatatta ccgccaacct
     1201 catcatcatg gctgttatag gactgcatta aattccagga aaggttattt ctttccagcg
     1261 ccgtaccgct gagtccaagt tgatgtgagc taccggaatt atcgttttgt gaaacagaat
     1321 aggtggcgta actatttccc caccagcccg ataaaggaac agataaagag agcatgtaga
     1381 tctgctcact ggtgttatca ctggcatggt tgttccccgt cccctgccgg gcagtattgg
     1441 aataactcca ggagacgttc cacgagagac gcttccagct actgctgtag ccaaattgca
     1501 ttaatcgttc aacatcatcg gtatgccagt actgctggcg acttaatgtc agatataagt
     1561 tgccatagtc tctgcccagt gattgcgtta acgttccttc cacacggctg cggcggttac
     1621 cggtttccca gaaatcttca ggattattac gccgcgacgc ccattcattg agagtatagt
     1681 aaccttcggt ggagtaacga tagccaacca ggcgcaagct ggtatccgta tcgtcaaagc
     1741 gtttcgaata cagaaagcga taagactgac cggtttccgt atcagcatgg ctaaatttcg
     1801 cacgggcatg tgtcacatcg aaagaaattg cacccagtgc gccaagctct ttaccgacac
     1861 ccaaagcggc agaaccatac ttttcagcag cctgcatacc tccatacaag gtgaaaccgt
     1921 agggaaaacc atgaaggatc tgcacctgaa gaacgtcagg ggtaaagcca tcttcatcgc
     1981 gtaattctcc cacactgaca tcaacatcag tttgaccttc acgtttgagg atggctaacg
     2041 acgcataagg ttgagtaaat cgttgttcgc taccgtcttc ttctttgatg gttacgtcca
     2101 gatcaccacc tgtactggcg gtgttgaggt cggtaatttc aaaagcacct tgtggaacgg
     2161 ctgactgata aatcacgtag ccattctggc gaataatgac agtggcgtta gactttgcaa
     2221 tcccacgcac tacgggggca aatccgtttt ggctggcagg caacatatcg ttatcggtat
     2281 acaagcgtgc gccacgaatt tgcgtactgt cgaagatatc gctcgccgtc cacgtatcac
     2341 ctatcataat ctggcttctt aacgcagcta tatcacgttg tatccaggtg cttatagagt
     2401 tgtattctgc actgccatcg gtcgtattta aggtgctgta attgcgtaat cgccacggcc
     2461 caatattcag tccattgcgc agattgagat aactggtatc tgtctcttgt gaaccataat
     2521 ccgaacgtag agtatgtgaa ccagaaaaat cgtagttgat ggtgagcgcg ttaatcccct
     2581 catcccaacg cgaggggctg atgtaatcat aagccatcgc atccatatag atttggggaa
     2641 tggagatatt cagttgcagt ttatcgctgt cgaactgcca tgatgagcct tcaatgcgtg
     2701 gcccaggatc aatacagact gtttcatcta catcattgag cgatttcagc gcatcaactt
     2761 ttacgccaaa ggtggttagc gtttttttaa tagcgacaaa gcaggcgtgt aaatcagcat
     2821 cgggattatc ctgaacaaag gtcacatcct gacgttcaaa cattttaccg ttgataaaaa
     2881 tatcaacgct ataaacacca gggggcatca ggtttgcccg attaaactgg cgaatatcga
     2941 ctccggattg atggtcgatt tccagaagcg ccggattgaa tacactgacc tcggccatca
     3001 ggctttgcga gaaaatcaga cacgtccctg ccgtgaggcc aaccactatt ctggtcgtca
     3061 tcataatacg cctatcctta tcccttgctt atagcgtcat ttttatcggg tcactgactg
     3121 cgccgtaatc attaataaat cgccaggtca tttgacctcc cgcagaatta gcaggtaaag
     3181 gtaacgtgag cgtttcaaaa ggacggatca tgttgagggg cacatcatga ccatcaacga
     3241 tgacatccat cacactgata tgaatcgggt tgggattctc tgcaatcagg tttcgccctt
     3301 cccggtgcca cttcaccttc ggtgcagcat cttcgggaat caggcgaatg cttgccggtc
     3361 gccagaacag cttgatacgc gttttaactg cgatttccag tgagttcgcc gctttgttgc
     3421 tcgcagaggg cggaatattt ttaatattca gccagaagac tgactcgcga tcatcaggca
     3481 atgacacacc tttggtatga ataatgcgta atactttttc ttgcgcgggt tctaatcgtg
     3541 ataccggagg aataacggta aacggtgctt tgcttttatc ctctttatta aacggatcta
     3601 cccatgcctg gatgagataa ggaatttttt cttccttgct aaatacagaa atagatttat
     3661 ctgggttatt acttaagtaa actacccgtg taccaccaat gacaacgcct gcataggtta
     3721 atgctggttg gactaaaaaa acactgagta taatcgcggt aaataactta catgaaacgg
     3781 ttgttttaaa gagcctgatt aaacaacttt ttctggacat aacgactccc tggccaaagc
     3841 caactgaata aaaaggccct tgataaatta tttcttatat tcgacagtaa attgtgaatc
     3901 ggcgttcgcc gtacccgccg ttacggttgc attggttgcc acatagcgtg caataaactt
     3961 catttcagcc ttgccttcag cgtcgatagc ggtataggca gaatcgttat aaagtttaac
     4021 ctgcgagtta tctgactggt taaaaatacg aataccgaca cccgttgccg caatcgcttg
     4081 tcctgtaccg gtaaaatcgc cagggttgcc cgctgtaagc ggcgtaccca ctttcaggtc
     4141 gccgtcgccg ccaggcgctt ctgtaccatc aaaacgaacg gctgcctgag tgtaggttgc
     4201 cgggcacttt tcaagtttaa tactgaactg ctgtggtgac gccagactac ctacacctga
     4261 aaatgcattt ttactgactt tacccagtgg gacggtttga tcggctgtat cggaggtaac
     4321 ttcacaagtc gtgtcgacaa tttcaccgac gaagtgaaca acaccatctt ctgccagtgc
     4381 atgtccggcc agcattaaag atgccagagt gaatgcaccg cctataatat tacgcttcat
     4441 aaaaactcca ttgatataat atatccttta ccatgaatca tcacttatat taataagtgt
     4501 atttttgatc caaaggaata gtacaccaaa gagacaactt tatccaaaaa taatattaat
     4561 cactcaatct taaatataaa ttattattta aatattatta aaacagcaag aaatcaagtt
     4621 tatt
//
LOCUS       DAZKJR010000244         4603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKJR010000244 DAZKJR010000000
VERSION     DAZKJR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.245"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHOWS_005003"
     CDS             complement(<1..593)
                     /locus_tag="ACHOWS_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0050443.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="ACHOWS_005004"
     tRNA            complement(708..783)
                     /locus_tag="ACHOWS_005004"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="ACHOWS_005005"
     tRNA            complement(790..864)
                     /locus_tag="ACHOWS_005005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="ACHOWS_005006"
     tRNA            complement(981..1065)
                     /locus_tag="ACHOWS_005006"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="ACHOWS_005007"
     tRNA            complement(1074..1149)
                     /locus_tag="ACHOWS_005007"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="ACHOWS_005008"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="ACHOWS_005008"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0050444.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHOWS_005009"
     CDS             complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHOWS_005009"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFQ0050445.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHOWS_005010"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHOWS_005010"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0050446.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1181 a         1157 c         1046 g         1219 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgccccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgcctg
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1921 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2461 atttaaaggg gagttaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccaacttttc ccagcgcgaa agataaggtg tcaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     3061 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3601 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3661 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3721 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3781 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3901 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     4021 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt accgcgatag tcttccagaa
     4321 aaagtacatt acttccttca cccagaataa gaacggattg tccttttgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tctgcggggg gatggcttgt agatatgatg acaggaagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactgggcc ttc
//
LOCUS       DAZKJR010000245         4461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKJR010000245 DAZKJR010000000
VERSION     DAZKJR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.246"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1688)
                     /locus_tag="ACHOWS_005011"
     CDS             complement(<1..1688)
                     /locus_tag="ACHOWS_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFQ0050447.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WAALLPAESVEEVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAATRPAVCDDGVAATSGGGEGKKRRYRKNNVSGLTAEMLDAWARKNLYAYQLHCREN
                     RHREWRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFALQ
                     FGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMAT
                     HDKYRTTYFSTPSAKTHQAYLVWNGDEWRGDDPARRAVEFPKESAMRVGCECPDGIWR
                     YIIRLEEAVAGGLSARVNIERIRNRYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEVE
                     METWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPLHKGERFRVLAVWQWQG
                     FNFSWQAEQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNRL
                     VMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMAIRRTTTKSGNALTFVAERS"
     gene            1855..2913
                     /locus_tag="ACHOWS_005012"
     CDS             1855..2913
                     /locus_tag="ACHOWS_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFQ0050448.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     MLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTTHAPSKPESFLKRLFSAGQTDNTDNTDTSKPDTGEDAKMDEIKALLEKLITLVE
                     GGSAAAAGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTA
                     AKAKLEDAIKAFSTITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGI
                     TPRPAGTPAESEKPFDFV"
     gene            2925..3944
                     /locus_tag="ACHOWS_005013"
     CDS             2925..3944
                     /locus_tag="ACHOWS_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFQ0050449.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGSENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEEA
                     EDVGA"
     gene            3962..>4461
                     /gene="gpM"
                     /locus_tag="ACHOWS_005014"
     CDS             3962..>4461
                     /gene="gpM"
                     /locus_tag="ACHOWS_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0050450.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTTWR
                     LHEEIRA"
BASE COUNT         1041 a         1388 c         1216 g          816 t
ORIGIN      
        1 gaccgttccg ccacgaacgt cagcgcgtta ccgctttttg tggtggtgcg gcgtatggcc
       61 ataaacgacg cggggatctc cgtgcgttca cggctggcct catcaatggc atctttcgcc
      121 cactcgatac gcttatgggc caccacgtct atcatcttca tcaccaggcg gtttttgctc
      181 tccacgctgt aaagaatggc gttagcctca cgcggcgcaa agcgactgac gatatcaaac
      241 acccctttcc cgatgccggt aatatcgatg ccgatatagg taatgttaaa gcgctgcatc
      301 agttcgcgga tctgttcggc ctgccagctg aaattaaatc cctgccactg ccagactgcg
      361 agcacccgga aacgctcgcc cttatgaagc ggcggagcca caatcacgaa cgtggaatta
      421 tccccggacc ttgacgggtc aaagcccgcc cacacctcac gattgccgaa cggacgtgct
      481 gcggtggggt cgtagtcccc ccacgtctcc atctccacct cgcacctgac cagttcggag
      541 aacttaaata ccgcatcctt gctgtcaaca aactcgcagc cgtacagcat cgcgtaggtg
      601 gtcgggttat agcgattcct gatacgttca atattgaccc gcgcggacag gccacccgcc
      661 accgcctctt ccagacggat aatgtagcgc cagatcccgt ccggacattc gcaccccaca
      721 cgcatcgcac tttctttcgg aaactcaacc gcacgacgcg ccggatcatc gccgcgccac
      781 tcgtcaccgt tccacaccag ataagcctgg tgggttttgg cgctgggcgt ggaaaagtac
      841 gtcgtgcggt atttgtcgtg ggttgccatt gccgaggcca cttcatgcag tcgcgtgaat
      901 tttgggatcc agaacacctc atcgccgtaa aggtggccgt taaatccctg ggcggtactg
      961 gcattggtgg acaggaaacg cagcattgcg ccattgctta aacggatatg cttacccgtg
     1021 agcgtgatcc cgaactgcag ggcgaaatta ataatatatt cgcggaagat ttccgcctga
     1081 acccgtgacg cagaaaagaa tatctggtta tcaccagtca ccaccgcatc ttcgaacgcc
     1141 tcccatgcaa aataccaggt catgcctatc tggcgactct tgaggatgaa acgccactca
     1201 cggtgccggt tctcgcggca gtgcagctgg taggcataaa gatttttacg cgcccatgca
     1261 tccagcattt cagcagtcag cccggaaacg ttgtttttcc ggtagcgccg ctttttccct
     1321 tcccccccgc cgctggtcgc cgccacaccg tcgtcgcaca ctgccggacg cgtggcggca
     1381 atttccgcca tcctttcagc gtgtttgttt ttctgggcga taatcttcac gtgctgtgcg
     1441 ataagctcgc gcagttcggc aagttcgcac tccgtcttac cctcgcgccc ggtcagcaca
     1501 tcaatacgcc gggcgatcac ctcctcaacg gattcagcgg gcagcagggc ggcccactgc
     1561 cccacatcac accagtggta aaccgtgcgc ggcggaatac cgagctccgc cgcaatatct
     1621 cggggatgcc accgcttcag ataaagcgag cgggccgcct ctttaatttc gtcagaatat
     1681 ttagccatgc gcccattatg gcggcgcatc cggcccgcat tcaggcagaa aatttcggct
     1741 gtgttcggct atcccacgtt atccgaagac agccgaattc agccagtagc cccccgctgt
     1801 aaaaatcccg acactcaccg gagttcttca gattttcacg taaaagaggc cacgatgccg
     1861 cagccaacgt tgatgaccga ctggatatgt atcgccacat caggaccgac cgttgacggc
     1921 cgcgaaattg atccgcagtg gctgactgat gccgctgaaa cctattcccg caacacctac
     1981 acggcgatgc tgtggccctg gcatgaggaa gaaatcggac accgccagta caccagtaat
     2041 ctgggtgaag tggactcact gaaagcggaa accggcgagg acggtaaaac ccgcctctac
     2101 gcgcggctgg tccctaacca gtacctgatt gaagccaaca gactggggca gaagctgttc
     2161 acatccgccg agatagtgga gaacttcgcc cggagcggac gcgactatct cctgggcgtg
     2221 gcggtgacgg atatccccgc cagtctgggg acggaaaaaa tacgcttcac cctgaacaac
     2281 gaggaataca cagcacagcg ggcgaactgc cagacgttca cgctgggcag cctgtcaccg
     2341 gacaccaccc atgcccccag taaacctgaa tcattcctga aacgcctgtt cagcgcagga
     2401 cagaccgaca acaccgacaa caccgatacc agtaagcctg atacaggcga ggacgcaaaa
     2461 atggacgaaa ttaaagcact gcttgaaaag ctgatcaccc tggtggaagg cggcagcgcc
     2521 gctgccgctg gcgagggcac agcagacacg ccggaagaag caaaagccga agtcgcggaa
     2581 atcgccgccg aaatcgccga cgccgccgca gaggtggcag agctggcaga agaagtgaaa
     2641 gccgccccgg aagacgaggt taaggccgaa gcctttaccg ctgcaaaagc gaagctggaa
     2701 gacgccatca aagccttcag caccatcaca ccggaaaaag gcgaacgtta ccgccgcttt
     2761 gccgcccgtc gccgggacga caccgacaaa gaagaaatca aagccattgc cgaaaaactt
     2821 aacgacctga ccacgaagct gtcagccacc ggcatcacgc cacgcccggc tggcacaccg
     2881 gcagaaagcg aaaaaccgtt tgactttgtt taaggacacg caacatgcaa ctgaaccaga
     2941 aaaccgaatc actgcttcgt aagtacgctg ccgggctggg cgcggcaaac ggggttgacg
     3001 acgtcacacg ctattttgcg ctgtctgacc cgaaggagac cgcgctgcgt gacgccatca
     3061 tgcactccga cgagttttta tcaaaactgc cgaacgtgct ggatgttgaa caggtgacag
     3121 gccaggtggt taccaccggc gttccgggcc tccataccgg acgtaaaaaa aatgcccgct
     3181 tcacccttcc gctgggtgtt accgggaatg aatacaagct ggctgaaacc gattccggcg
     3241 cccacctcct gtacaccaca ctggtcacct gggcgaacgc aggaagtgaa aacgagttct
     3301 ttaaccgcat tcagaatttc gttaacagac agattgcgct ggatatcctg cgcatcgcct
     3361 ttaacggtac cagcgtggcc gaagataccg acccggtaac caacaaaaac ggcgaggacg
     3421 tcaacaccgg ctggcatgcc atcgtcaaag gccgcagcgc aaaacagatc gtggaggctg
     3481 gcccgacgct gggcaagggt ggcgacttta tcgggctgga tgcagccgtg gcagacctcg
     3541 tgcataccct cattgatgag tcgtatcgcg gtcatccgga tctggtggta ctggttgcgc
     3601 ccgatcttgt ggcacaggac aaggtgacgc tgcttaacgc catcgacgcc cccacggaaa
     3661 aagtggccgc gcagcttatc ggtcgccaga tcgccgggct gacagcatac accccgccgt
     3721 tcatgccgga agggcgcctg attgtcacca cgctggcaaa ccttcacatc tacacgcagc
     3781 gcggcacccg tcagcgccgt gcggagtgga acgacgatcg caagcgtttt gaaaataact
     3841 atctgcgcat ggaaggttac gccgtggagc acgacgaaat ttacgcggcc tacgacaagc
     3901 tgaccatcaa gggcgcagaa gaagccgaag acgtcggcgc ataagcacag agggcggcgc
     3961 aatgacaccc tgccagcgac accgggcgaa aatccgcaca caggaggcac tcgaacgccg
     4021 ggaggcgctg acggcctccc cggtcagctt ccacctgtta cgcgcggagc ttgatcgcga
     4081 tgtggcacgg ctgcgcagtc ttccggtccg tgaagagcgg ctggcgttca agcgcgacat
     4141 tctcctgccg cgctggctgc ccgtcgcgga gcgctatatc gctgacggaa agcgccacgc
     4201 ctgcccggta ctggtgtact gcatcatctg gctgttcgac acgggcgacc tgagtcgcgc
     4261 ccttgactgg gcagacattg ccatcagtga aggccaggcc acgccggaca acttccgtag
     4321 caccctgccc gccttcgtgg ccgacacggt gctgcagtgg gccaccgaca acagcgaggc
     4381 cggacacagc atagagccgt atttttcgcg caccttcagg aacgtcacca ccacgtggcg
     4441 gctgcatgag gaaatccgcg c
//
LOCUS       DAZKJR010000246         4342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKJR010000246 DAZKJR010000000
VERSION     DAZKJR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.247"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..1407
                     /gene="pepQ"
                     /locus_tag="ACHOWS_005015"
     CDS             76..1407
                     /gene="pepQ"
                     /locus_tag="ACHOWS_005015"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFQ0050451.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENITRDLKLA"
     gene            1407..2021
                     /locus_tag="ACHOWS_005016"
     CDS             1407..2021
                     /locus_tag="ACHOWS_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFQ0050452.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            2060..3511
                     /gene="trkH"
                     /locus_tag="ACHOWS_005017"
     CDS             2060..3511
                     /gene="trkH"
                     /locus_tag="ACHOWS_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFQ0050453.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            3523..4068
                     /gene="hemG"
                     /locus_tag="ACHOWS_005018"
     CDS             3523..4068
                     /gene="hemG"
                     /locus_tag="ACHOWS_005018"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFQ0050454.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         1008 a         1085 c         1200 g         1049 t
ORIGIN      
        1 ggcagaaatc acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa
       61 acagaagggt aaaaaatgga atcactggcc tcgctctata aaaatcatat agctacctta
      121 caggaacgga ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc
      181 ggcgaactgt tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa
      241 ttcaaagcgt gggtgccagt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg
      301 aataagccga aactgtggtt ctatctaccg gttgattact ggcacaacgt cgaaccgctg
      361 ccgaactcct tctggactga agatgtagaa gtgatcgcgc tgccgaaagc cgatggcatt
      421 ggtagtctgt tgcctgctgc gcgcggcaat atcggttata tcggtccggt gccagaacgt
      481 gcgctgcaac tgggtattga ggccagcaat atcaacccga aaggggtgat cgactacctg
      541 cattactatc gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa
      601 atggcggtca acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc
      661 gatatcaaca ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttatagc
      721 aacattgtgg cgcttaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag
      781 gcaccggaag agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg
      841 gctgatctaa cccgtacctg gtcggcaaaa agcgacaacg actacgcaca gctggtgaaa
      901 gacgtaaatg atgaacaact ggcgctgatc gctaccatga aagcaggcgt cagctatgtg
      961 gattaccata tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc
     1021 accgatatga gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac
     1081 ggtatcggcc atccgctggg cctgcaggtg catgacgtag ccggttttat gcaggatgat
     1141 agcggtacgc acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc
     1201 cagccgggca tggtgttaac catcgaaccg ggtatctact tcatcgaatc gcttctggca
     1261 ccgtggcgtg aagggcagtt cagcaaacac ttcaactggc agaaaattga agcactgaaa
     1321 ccgttcggcg gcattcgtat cgaagacaac gtggtgattc acgaaaacaa cgtggaaaac
     1381 attacccggg atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca
     1441 cggtcgttga agagatcaaa aagagccgct tcattacgct gttggcgcat accgatggcg
     1501 ttgaggcggc aaaagcgttt gttgaatcgg tgcgggcaga gcaccccgat gcccgccacc
     1561 attgcgtggc gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg
     1621 acggggagcc ggcgggaacg gcaggtaaac cgatgctcgc ccaactaatg ggcagcggcg
     1681 tcggggaaat taccgctgtg gtggtgcgct actacggcgg catattgctt ggcaccggtg
     1741 ggttagtgaa agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac
     1801 gcaagacacc attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg
     1861 aagcgttgct ggggcagtgt gacggcaaaa ttattaacag tgattatcag gcattcgttc
     1921 tgctgcgggt ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt
     1981 ttagccgtgg ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag
     2041 aactaaggaa gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt
     2101 catcttattt tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg
     2161 agcgggccgc gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg
     2221 gtggccgaac cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt
     2281 gctgttctgg accgtgctgg gtagcgtcgg tgcgctccct tttatcttct cggaaagccc
     2341 gaacctcacg attaccgatg cgttttttga atctttctct ggcctgacca ccacgggggc
     2401 cactacgcta gtggggctgg attcgctccc tcacgccatc ctcttttatc gccagatgct
     2461 gcaatggttt ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg
     2521 cgtgggtggg atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat
     2581 gcgcccgcga attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt
     2641 cgcctgtgcg ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag
     2701 ctttgcgact atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga
     2761 cagcccgact attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg
     2821 tctgcacttt tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt
     2881 tcgcatgttt atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt
     2941 tcataatgtc tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc
     3001 gatggcgaca accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc
     3061 ggtactgctc ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct
     3121 gaaagtgatc cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt
     3181 gcatccgaac gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct
     3241 cgaagccgtg tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc
     3301 gattatcgcc acgggcgtgg atgacttttc tgcctttgcc tcggttgttg cgacattgaa
     3361 taacctgggg ccagggcttg gtgtggttgc tgataacttt accagtatga acccggtggc
     3421 taaatggatc ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt
     3481 gctctttacc ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt
     3541 ttctcaacaa gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa
     3601 gaactgggga tccaggcgga tgtcgccaat gtgcaccgca ttgaagaacc acagtgggaa
     3661 aactacgacc gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc
     3721 caggagtttg tcaaaaagca tgcgacgcga ctgaattcga tgccgagcgc cttttactcc
     3781 gtaaacctgg tggcgcgtaa accggagaag cgtactccac agaccaacag ctacgcgcgg
     3841 aagtttctga tgagttcaca atggcgtccc gatcgctgtg ccgtcattgc cggggcgctg
     3901 cgttacccac gttatcgctg gtacgaccgt tttatgatca agctgattat gaagatgtca
     3961 ggcggtgaaa cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat
     4021 ttcgcccgag aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata
     4081 aaaaatgtgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct
     4141 ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct
     4201 cctgagaatc tcaacagaga aaagcaaaaa aatgcttgac tctgtagcgg gaaggcgtat
     4261 tatgcacacc ccgcgccgct gagaaaaagt gaagcggcac tgctctttaa caatttatca
     4321 gacaatctgt gtgggcactc ga
//
LOCUS       DAZKJR010000247         4224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKJR010000247 DAZKJR010000000
VERSION     DAZKJR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.248"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            302..910
                     /locus_tag="ACHOWS_005019"
     CDS             302..910
                     /locus_tag="ACHOWS_005019"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFQ0050455.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1008..2399
                     /gene="selA"
                     /locus_tag="ACHOWS_005020"
     CDS             1008..2399
                     /gene="selA"
                     /locus_tag="ACHOWS_005020"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFQ0050456.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2396..>4224
                     /gene="selB"
                     /locus_tag="ACHOWS_005021"
     CDS             2396..>4224
                     /gene="selB"
                     /locus_tag="ACHOWS_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFQ0050457.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDAL"
BASE COUNT          946 a         1075 c         1240 g          963 t
ORIGIN      
        1 cggctgaacc ctggacaatg ctaccgccat actgcgtcat gtcgccctga cgcgccgccg
       61 gttttccgct catctgctca tccttgtttt atcgggttat ttgtctgctg acaggcgaaa
      121 ccctacagat ttataagaaa gccttaagtg agatatctgt cagataagaa caattaggaa
      181 aatatttata tcaccatgat aaatatgtga tttattggga agtaacaaac catcaacatt
      241 ttctgccctt tttcctatca ccaggccata ctgatatttt tcctcgcaca aaggactcgg
      301 catgaaactc gtcggtagct acaccagccc gtttgtacgc aaactttcta ttctgttgtt
      361 agaaaagggc ataactttcg aatttattaa tgaactgccc tataacgcgg ataacggcgt
      421 ggcgcaattt aacccgttag ggaaagtgcc ggtgctggtg accgaagagg gcgaatgctg
      481 gtttgattcg ccgatcatcg ctgaatatat tgaattaatg aatgtcgctc cggcgatgtt
      541 gccgcgcgat ccgctggagt cgttgcgggt gcgcaaaatt gaggcactgg cggatggcat
      601 tatggatgcc gggctggtat cggtgcgtga acaggcgcgt ccagcggcgc agcagtctga
      661 agatgaattg ttacgccagc gcgagaaaat caaccgcagt ctggatgtgc tggaaggata
      721 tctggtcgat ggcacgctca aaaccgatac ggtcaatctg gcgactatcg ccattgcctg
      781 tgccgtcgga tatctcaatt tccgccgcgt tgcgccaggc tggtgtgtcg accgcccaca
      841 cttagtcaaa ctggtcgaaa acctgtttag ccgcgaaagt tttgctcgca ccgaaccgcc
      901 aaaggcttga tgcgcgatat gtcctcctga cccatctcac gttacaatcc gtggttatgt
      961 taaacgccct tctccgtgtg agagggcctt gatcagccag gtttcctatg acaaccgaaa
     1021 cgcgttccct ctatagtcaa cttccggcta ttgatcgctt attgcgcgat agctccttcc
     1081 tttctttgcg tgatacttat ggtcacaccc gcgtggtgga attgctgcgt cagatgcttg
     1141 acgaagcgcg agaagtgatt cgtgatagcc agacgctgcc cgcatggtgt gaaaactggg
     1201 cgcaagaagt cgatgcccgg ttgacgaaag aggcgcagag cgcgctgcgt ccggtgatca
     1261 acctgacggg aaccgtgctg cataccaatc ttgggcgagc tttacaggca gaagccgcgg
     1321 tggaagccgt tgcgcaggct atgcgttcgc cagtgaccct cgagtacgat ctggacgacg
     1381 ccggacgcgg acatcgcgat cgggcgctgg cgcagctgct gtgccgcatt acgggagcgg
     1441 aagatgcctg tatcgttaat aacaatgcgg cggcggtgtt attgatgttg gcggccactg
     1501 ccagcgggaa agaggtggtg gtatctcgcg gcgaactggt ggagattggc ggcgcgtttc
     1561 gtattcctga tgtcatgcgt caggcaggct gcaccctaca cgaagtgggg accaccaacc
     1621 gcacgcacgc taatgattat cgtcaggcgg tgaatgaaaa taccgcgctg ttgatgaaag
     1681 tacataccag taactacagc attcagggat tcaccaaagc gatagatgaa gcggaactgg
     1741 tggcgctcgg caaagagctg gatgttcccg tagtgactga tttaggcagt ggctcgctgg
     1801 ttgatcttag ccagtacggt ttgccgaaag agccaatgcc gcaggagttg attgcggcgg
     1861 gcgtcagtct ggtgagtttc tccggtgaca agttgttagg cgggccgcag gcaggaatta
     1921 ttgttggtaa aaaagagatg atcgcccgcc tgcaaagcca cccgctgaag cgtgcattac
     1981 gcgcggataa aatgaccctc gcggcgctgg aagccacgtt gcgtctttat ttacaccctg
     2041 aagctctgag tgaaaaatta ccgaccctgc gcctgcttac ccgcagcgca gaggtcattc
     2101 aaatccaggc acaacgttta caggctcccc ttgccgcaca ttacggcgcg gagtttgcag
     2161 tccaggttat gccatgtctt tcgcagattg gcagtggttc actgccggtt gatcgcctgc
     2221 cgagcgcggc attaacgttt acaccccatg atggacgcgg tagccacctt gagtcattag
     2281 ccgcccgctg gcgtgaattg ccagtgccgg tgattggtcg tatttatgac ggacgattgt
     2341 ggctggattt acgctgcctt gaagatgagc aacggttttt ggagatgttg ttgaaatgat
     2401 tattgcgact gccggacacg ttgaccacgg caaaacaacc ttattgcagg cgattactgg
     2461 cgtaaatgcc gatcgtctgc cggaagaaaa aaagcgcggc atgactatcg atctcggcta
     2521 tgcctactgg ccgcagccgg atggccgcgt gcctggtttt atcgacgttc ccggtcatga
     2581 aaagtttctt tccaacatgc tggcgggcgt cggtggtatc gatcacgcgc tgttggtggt
     2641 ggcgtgcgat gacggtgtga tggcacagac ccgtgagcat ctggcgattt tgcagctgac
     2701 tggtaacccg atgctgacag tggcgctgac caaagccgat cgcgtggacg aagcgcgtgt
     2761 tgatgaggtt gaacgccagg taaaggaggt gctgcgggaa tacggttttg ctgaggcaaa
     2821 actgtttatc accgcagcaa ccgaaggtcg gggaattgat gccctgcgcg agcatctgct
     2881 tcagttacca gaacgcgaac acgccagcca acatagtttc cgcctcgcga tcgaccgtgc
     2941 atttaccgta aaaggtgccg ggctggtcgt caccggtacg gcgttaagcg gggaagtgaa
     3001 ggtaggcgat tcactctggc tgactggtgt aaataaaccg atgcgcgtgc gtgcgctgca
     3061 tgcgcaaaat cagccaacag aaactgccca tgccgggcag cgtatcgcgc ttaacatcgc
     3121 gggtgatgcg gaaaaagagc agattaaccg tggcgactgg ctgcttgccg atgcgccgcc
     3181 ggagccgttc acacgggtga ttgtcgagct tcaaacccat acaccgctga cccagtggca
     3241 gccgctgcat attcaccacg ccgccagcca cgtcacggga cgcgtttcgc tgctggaaga
     3301 taaccttgcc gagctggtgt tcgacacgcc gctgtggctg gcggataacg atcgcttagt
     3361 gttgcgcgat atctcggccc gcaacacgct ggcgggggcg cgtgtggtga tgcttaaccc
     3421 gccgcgtcgc ggtaagcgta agccggaata tctgcaatgg ctggcgtctc ttgcacgggc
     3481 gcagagcgat gccgatgcgt tatctgttca tctggaacgc ggagcggtta accttgcgga
     3541 tttcgcctgg gcacggcagc tcaacggcga agggatgcgc gaactgctgc aacagcctgg
     3601 ctatattcag gctggttata gcttgttgaa tgcgccggtt gccgctcgct ggcagcggaa
     3661 aattctcgac acattagcga cttatcatga gcaacatcgc gatgaacctg gtcctgggcg
     3721 agaacgtctg cgacgtatgg cgttgccaat ggaagatgaa gcgctggtac tgttgctgat
     3781 tgaaaagatg cgcgaaagcg gcgacatcct cagtcatcac ggctggctgc atctgccgga
     3841 tcacaaagcg ggcttcagcg aagagcagca ggccatctgg caaaaagcag agccactgtt
     3901 tggtgacgaa ccgtggtggg tgcgagacct ggcaaaagaa acgggaaccg acgagcaggc
     3961 aatgcgcctg acgctacgcc aggcggcgca acaggggata attactgcga tcgtcaaaga
     4021 tcgttattac cgtaacgatc ggattgtcga atttgccaat atgatccgcg atctcgatca
     4081 ggaatgtggt tcaacctgcg cggcggattt ccgcgatcgc ttaggcgtag gccgaaagct
     4141 ggcaattcaa atcctggaat attttgaccg cattggcttt acgcgtcgtc gtggaaatga
     4201 tcatttattg cgcgacgcat tatt
//
LOCUS       DAZKJR010000248         4179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKJR010000248 DAZKJR010000000
VERSION     DAZKJR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.249"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..694)
                     /gene="reiD"
                     /locus_tag="ACHOWS_005022"
     CDS             complement(<1..694)
                     /gene="reiD"
                     /locus_tag="ACHOWS_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HFQ0050458.1"
                     /translation="MAYVSQVSESNGPFVRFMHMHPRTVELILITEGKGEYFIGDRIY
                     PVRKGDLVIYNSEVVHDEYLESGRPVGTICCGINNIVCPGLRENALIPDDTVPVIPLY
                     HHYATIEKLMSSIFNVINQHTVEGPTMAQLLTRVLLKYVEGNVLMRTGNDITRRHKNL
                     LDSIKAYIDHNFYEPIRLDALATKFNVSPYYVSHEFKRRYGYSPMDYLIKRRLGEAQS
                     LLTTDEGGCEKIT"
     gene            1125..2630
                     /gene="iolA"
                     /locus_tag="ACHOWS_005023"
     CDS             1125..2630
                     /gene="iolA"
                     /locus_tag="ACHOWS_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate-semialdehyde dehydrogenase IolA"
                     /protein_id="HFQ0050459.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQPLGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLTSDPRI
                     QAVSFVGSSSVAEHIYTTASAHGKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGS
                     AGERCMALPVVVAVGDETANRLIERLKPLIAALRIGPGELREKDENEMGPVVSRMHQQ
                     KVLAYIDKGVSEGATLVMDGRNYCVKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVL
                     GIVRVTDYATAIRTVNSHEFGNGSVIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAF
                     HSFGGWKRSVFGALNVHGPDGVRFYTRMKTVTARWPNGQQIASEFSMPTLG"
     gene            2655..3464
                     /gene="iolB"
                     /locus_tag="ACHOWS_005024"
     CDS             2655..3464
                     /gene="iolB"
                     /locus_tag="ACHOWS_005024"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFQ0050460.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            3696..3863
                     /locus_tag="ACHOWS_005025"
     CDS             3696..3863
                     /locus_tag="ACHOWS_005025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050461.1"
                     /translation="MPYTAACYSGFLTGSIRWAVYREIIRFSVEVEVEVEVEVEVEVE
                     VEVEVEGVYRS"
     gene            3866..4176
                     /locus_tag="ACHOWS_005026"
                     /pseudo
     CDS             3866..4176
                     /locus_tag="ACHOWS_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1132 a          961 c          958 g         1128 t
ORIGIN      
        1 aggtaatttt ttcacaccca ccttcatcag tggtaagtaa agattgtgcc tcccctaacc
       61 gacgtttaat caaatagtcc attggcgaat atccatagcg ccgtttaaat tcatgagaaa
      121 catagtaagg gctgacatta aacttagtcg ccagagcatc cagacggatg ggttcataga
      181 aattatggtc aatataagct tttattgaat ccagtaaatt tttatggcgc cgtgttatat
      241 cattacctgt gcgcattaat acatttccct cgacgtattt aagcagcacc ctggtcagca
      301 actgcgccat tgttggtcct tctactgtat gctgatttat aacattaaat attgaactca
      361 tcaatttctc gatcgtcgca taatgatgat atagtggaat tacgggcaca gtatcatctg
      421 gaatcagagc attctcccgc agacccgggc acacaatatt gtttatccca cagcaaatag
      481 tacccacagg ccgtccgctt tcaagatact catcatgcac tacttcgctg ttataaataa
      541 ccaaatcacc ttttctgact gggtaaatgc gatcaccgat gaaatactcc ccctttccct
      601 cggtgataag gattagctcc acggtcctgg gatgcatatg catgaaccgt acaaacgggc
      661 cattgctttc gctaacctga ctgacatacg ccaactgagc ccgggactta ttatcttgtt
      721 ctgaaggaca gaagcaaaaa cgcatgattt ttcctccatt atccgaatct gtagtgctac
      781 ggccaaaact aaaactattt ttttataata taaaaaaaca gaataaaacg ttacatagat
      841 catattattt tctggtgaaa ctcatctaac acggataaga tcatcgaata aaacaataca
      901 gaatcacaat ttattttctt tgatattcaa atagttaaca acaagcaacc tataaccatc
      961 accccccatg cagtgaactt attttttcat aaatttaaat ttgaaattta taatccgaag
     1021 ctatacatta aacactgtgt gattttagac tgtagatgat caggctttgc ctgtgaacac
     1081 tcagtcggaa cgtcgttaac attgattcaa gaaggtaaac agtgatggaa acagttggaa
     1141 attttattca gggtaaaacg agactgagca acagtggtga aacactaccg gtgactaacc
     1201 ctgcaaccgg agaagtgatt cgcctggtta cccagagcac ccggcaagat atgctggagg
     1261 cgattcagag tgctcatgaa gcttttcctg catggagtaa aatggcaccg ttgcgccgtg
     1321 caagaatcct ttttgaattt aaagtgttgc tggaaaaaca ccgagatgaa ctggcggctc
     1381 tgatcgtcag tgaacatggc aaagtatggt ccgacgcgct tggtgaactg actcgcggaa
     1441 tcgaagttgt tgaattcgcc tgcggcattc cgcatctcat taaaggagag ttctctttta
     1501 atgttggcac aggcgttgac agcttttcat taatgcaacc acttggtgtt gtagctggca
     1561 tcacaccctt taacttccct gcaatggtgc caatgtggat gttccctatc gctctcgcct
     1621 gcggcaatac cttcgtcctg aagcctccag cgctggtgcc ctccgcatcg ttacgtatgg
     1681 cgcaactact acaggaggcc ggactaccgg atggtgtatt caatgtagtt cactgtggca
     1741 atgacgcagc cagcctgttg accagtgatc cacgcattca ggccgtcagt tttgtcggat
     1801 cttcttccgt cgctgagcat atctacacca ccgccagtgc acacggtaag cgagtccaag
     1861 ctttcggtgc cgcaaaaaac cacgctattg tcatgccgga tgctgacctt gatgctaccg
     1921 ttaacgctat tatgggtggg gcttttggtt ccgcaggcga acgttgtatg gcattacctg
     1981 tggtggtcgc tgtcggtgat gaaacagcca accgtcttat tgaacgccta aaaccactta
     2041 ttgcagcgct gcgtattggc cccggtgagt tacgagaaaa agacgaaaat gaaatggggc
     2101 cagttgtttc acgtatgcat caacaaaaag tccttgcata tatagacaaa ggcgttagcg
     2161 aaggagcaac actggtgatg gatgggcgaa attattgcgt gaagggctat cctgaaggat
     2221 tttatgtcgg cggtaccttg tttgataacg tcacgccgga aatgactatc tggcgtgagg
     2281 aaatttttgg tcccgtgctc ggcatcgtac gcgtaacaga ttatgccacc gccattcgta
     2341 cggtaaactc tcatgaattt ggcaatggta gcgtgatctt cacctccaac ggccattatg
     2401 cccgcgaatt tgctcaatcg gttgaagccg ggatggtcgg tatcaatgtt ccggtaccgg
     2461 ttccaatggc gtttcactct ttcggcggct ggaaacgctc ggtgtttggg gcgctgaatg
     2521 tccacggacc ggatggggtg cgtttttata cccggatgaa aaccgttacc gcacgctggc
     2581 cgaacggtca gcagatcgca tccgagttca gtatgcctac gctaggttaa taaacgttca
     2641 ggaaggagaa gattatgacc aacttgctga gtacctgcac caccagggct ggaaatattc
     2701 aacatatttc tccgcaaaac gcaggctggg agtatgtcgg ttttgatgtc tggctgttga
     2761 aagccggcga atctttaacc ttgccatccg atgatcgcga acgttgcctg gttctggttg
     2821 ccggacttgc cagcgtaaag gccgctgacc acttttttta tcggattggc cagcgcatga
     2881 gtccttttga gcgaattccg gcttattctg tttacctccc gcaccataca gaagcaaaag
     2941 tcactgccga aactgatctg gaactggctg tctgcagtgc tcctggattc ggtgaacttc
     3001 cggtaagact gatctcacca caagatattg gggtggaaca taggggaaaa gggcgcaatc
     3061 agcgcttggt tcataatatc ctcccagaca ctcaacctgc tgacagtctg ctggtagtgg
     3121 aagtatatac aagggaaggc gatacaagtt cctggcctgc ccataagcat gacacagcag
     3181 ttgaagggca ggaaacctat ctggaagaaa cctactacca ccgtttcgat ccaccccagg
     3241 gattcagctt acagcgagtc tacaccgccg atcgcagtct ggacgaatgt atggcagtat
     3301 ataaccgcga tgtggtaaaa gtcccgaaag gctatcatcc ggtggccact atcgcaggtt
     3361 acgataacta ttatctgaat gtgatggccg ggccattacg caaatggcaa ttcacatggg
     3421 aagctgatca cgcatggatt aattcgtcag agtatccgcg ttaacaatac gttaaatatt
     3481 cctttgccag ttgtcctctg cagctggctt ttttgtgcac agtccccaac agggttctgc
     3541 ctcttataca caaatccaca ggcaacagaa tcggtggatt tttttgaatt ttttctctgt
     3601 ctgataatat cctgtttctg gtgttctgtt atggtttttt ctgattctta ctcctgagct
     3661 gacaatattt ttggttctgc cgaattagga gagaaatgcc gtacacggcg gcttgttact
     3721 ctggcttcct aacgggctca atacgttggg cagtctatcg tgaaatcatc cgtttctcag
     3781 ttgaagttga agttgaagtt gaagttgaag ttgaagttga agttgaagtt gaagttgaag
     3841 ttgaaggagt ctatcgcagt tagctctgaa aacatcaatg tctcgtaaag ggaactgtta
     3901 cgacaaaacc cctatggaaa gcttttgggg aactctgaag aatgaaagtc tgaaccacta
     3961 ccattttagt agccggaatg aagtcatctc agtaatactg ggtatacatt gagatattct
     4021 acaaccgtca gcgccgccac tcccgtctgg ggaatatatc cccggaaagc ttcaggaaaa
     4081 tattatcaaa tggcagctaa aaagaacaaa tggttgtgtc caccatgcca gcacacctca
     4141 cagaataaaa gcaccaacat gaaaaagata atatagccg
//
LOCUS       DAZKJR010000249         4146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKJR010000249 DAZKJR010000000
VERSION     DAZKJR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.250"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..801
                     /gene="tssC"
                     /locus_tag="ACHOWS_005027"
     CDS             <1..801
                     /gene="tssC"
                     /locus_tag="ACHOWS_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFQ0050462.1"
                     /translation="AFRETDDARYIGLVMPRVLGRLPYGPDTVPVRSFNYVEEVKGPD
                     HHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIP
                     SEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINAR
                     LPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGLVTEMTDPGDELQASHP
                     LRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            817..2154
                     /gene="tssK"
                     /locus_tag="ACHOWS_005028"
     CDS             817..2154
                     /gene="tssK"
                     /locus_tag="ACHOWS_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFQ0050463.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCKDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            2151..2804
                     /gene="tssL"
                     /locus_tag="ACHOWS_005029"
     CDS             2151..2804
                     /gene="tssL"
                     /locus_tag="ACHOWS_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFQ0050464.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            2807..>4146
                     /locus_tag="ACHOWS_005030"
     CDS             2807..>4146
                     /locus_tag="ACHOWS_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFQ0050465.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWYCLSSRRDAPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLA"
BASE COUNT          820 a         1055 c         1246 g         1025 t
ORIGIN      
        1 gctttccgtg aaacggatga tgcccgctat attggtctgg tgatgccacg tgttctggga
       61 cgcctgccat atggtcctga taccgtacct gtccgcagct ttaactatgt tgaagaggtg
      121 aaaggtccgg atcatcataa gtatctctgg acaaatgcgt cttttgcttt tgcggccaat
      181 atggtgcgca gttttgtcac caacggctgg tgtgttcaga tccgcggtcc tcaggccggc
      241 ggtgcggtgc aggatttgcc gatccatctg tatgatctgg gaaccggtaa tcaggtcaaa
      301 attccgtcag aggtgatgat tccggaaact cgggagtttg aattcgctaa tctgggcttt
      361 attccgctct cttactacaa gaaccgggat tatgcctgct tcttctccgc taactccacc
      421 cagaaaccgg cactgtacga tacgccggat gcaacggcga acagccgtat taatgcccgt
      481 cttccctata tcttcctgct gtcccgcatt gcgcattacc tgaagatcat tcagagggag
      541 aatatcggta ccaccaaaga tcgccgtctg cttgaactgg aactgaataa ctggatccgg
      601 ggtctggtga cggagatgac cgatccgggt gatgaactgc aggcctcaca tccgctgcgt
      661 gacggaaaag tggtggtgga agacatcgaa gacaacccgg gatttttccg cgtgaaactc
      721 tatgctgtac cacatttcca ggtggaaggt atggacgtca gtctgtcact ggtttcgcag
      781 atgccgaagg caaaagcgta aggcaggcgt cgggaaatga agatttatcg tccactgtgg
      841 gaagacgggg cttttctgat gccccagcag tttcagcagc aggctgcctg ggatgttcat
      901 ctggcagaca gcgttgcccg aatggggctg gcgcatccct ggggcgtggt ggctgcagag
      961 tttgatgact ctctgttacc gctttcacgg ttgaatgcca cccgtctgat tgttcgtttc
     1021 ccggacggaa cgcttattga tacggaacgt gcggataatc tgcctccggt ctgtgattta
     1081 tcgacggttt ctgagcgttc cctggtggat attgtgctgg cgctaccgtt actgaatgct
     1141 aacggtggca accttgatga tggcagtgag agcgagcgtc cgcgtcgctg gaaatcagaa
     1201 agggtgaatg tgcaggagct ggccgggcat gagcaaagcg aagtggcggt attgcggcat
     1261 aacctgactc tgcgcatggc tcatcaggaa aatgctgcct ggctgacctg tcctgttacc
     1321 cgtcttgttc gagatgcaca gggacagtgg tgcaaggacc cgcgttttat tccccctctg
     1381 ctgacgctgt ctgccagtcc ttcgctgatg actgagcttg cggagttgct tcatcacctg
     1441 caggcgcgtc gccagcgact gatgtccatg cgtcgggaga acaatgcccg tcttgcggat
     1501 tttgcagtgg cggacgtgtc gttgttctgg ttgctgaatg ccctgaacag tgcggagcct
     1561 gttctgaaag agttgcttga catgccttac cgccacccgg aactgttgta cagggagctt
     1621 gcccggctgg caggctccct gctcacattc tcgcttgagc ataatgtgga tgcggttccg
     1681 gcataccgtc atgaaacacc tgaaaatgtg tttccaccct tgctttcttt gctgaaccag
     1741 ctgctggagg caagcctgcc ttcccgtgtg gttttcattg aactgaaaca aaatggtgtg
     1801 atgtgggagg gggcattaca tgatgcgcgt ttgcgcgaag gggcggattt ctggctttca
     1861 gtgcgctctt ccatacccgg gcatgaactg cagacgaagt ttccgcagct atgcaaagcc
     1921 ggaagccccg atgatgtgtc tgaggtggtg aatgtggcgc tgagcggggt cattatccgt
     1981 ccggttacgc atgtaccggc ggccattccg ctccggctgg aaaaccagta tttcgcgctg
     2041 gatttaagta cggatgcagc ccgggcgatg ctggacgcgg ggcgttgtac tttctacacc
     2101 ccggcatcgc tgggggatgt gaaactggaa ctttttgcgg tactgcggac atgaataaac
     2161 ctgttatctc ccgggctgaa cagatttttt atcccggctg gctgatggtc agccagctgc
     2221 gctgcggaca accggttgag gatggtaagg ccctgtatcg ccgtgcctgt cagctggtga
     2281 cgcaggcgcg tgaagagctg gctgaagcag aattcagtca ggaaagcagt gaaatcatgt
     2341 tgtatgcctt ctgcgccctg ctggacgaga gtgtgctgaa ccgtgaaaaa acagacgatg
     2401 gctggcacac ctggcagcag gacccgctgc aggctcattt ttttggtacg ctcaatgccg
     2461 gagaagagct ctgggagcgt attcgtgagc aactgaaatt acctgcgccg gatgtggcgg
     2521 tactgacatg cctctgccgt acgcttcagc tcggttttac cggtcagtac cggtcgcagg
     2581 atgatgagcg tcgcgaagat gtaattcgcg cactgacagc gcgtgttcct gcatttactt
     2641 tcgcacagga tgcgccggtg gtggtgcgtg caccgggtta ccgtgctggc agaacgatgt
     2701 actggttgtc atgggtggcg ggtattgtga cgctggctgg tctctggtgt gtgctttctt
     2761 ctgtgctcgc agaccaggtg gcacggctta ccgggcaggg ataaccatgc acgtacgttc
     2821 ccgtctctgg ctgacaatac tgagtctgtt cctgacactg tggctgattt cgggattcct
     2881 gccactgtct gcagtaaacc agataatact gaccctggtt tcagttgtgg cggcgggact
     2941 ggtgctgtgg taccagtggt attgtctgtc ttcccgtcgt gatgccccgg agaaggctgg
     3001 cgatgacggt ctgccgcctg aatattttca gggacatgtg ctgcttgttg ccggaaactc
     3061 agatgcgtgg ttcgaacccg gtcaggcata ccgggaaaca gcatcgggat ggtatcttcg
     3121 tgtggatacg ccggagcaga tgagaatact ggcagagcgg ttgtcgtaca gccgtccggc
     3181 cctgtgtggc caggtcgtgg tcatgctggg acttttaccg gagcagcata cctcagatga
     3241 agctttcctg tcgtggttgc ggaactggca gcgtgcagtc atgcagtgcc ggcagtggct
     3301 gaataccatg ccgccggtgc tggtgacatt gatggtttca gggccgtcac cacaaaacac
     3361 atccttaaat aagcactctg cacactggtt tacactgaca ccggacctgt cgggaatgca
     3421 catacgcctg acggaagccg gagccattcc ggcagaggaa tggctccggg agtgtgatgc
     3481 agcgtccagg ctccggtgcg tcagtgaact gttatggctg aatgccatgt tgtcctggta
     3541 caaccgggtt actgatgccc tgacagatat ttgccggcag gagcactttt atttccggcc
     3601 ggttgccgtt ggattctgtc tgacgtcggt tgcggcccgg cccggtaatc tctggcaaca
     3661 acaactgacg tcactcactg tactgccacc gcctgccggc gtgtcatcac aggcactgcc
     3721 gttgccggat attctgctgt ccggtattcc ccgtcatcgt ggactcagtc actggcacag
     3781 actttgtctc cagacggggg cgattgtcgg cgtttttctt ctgctggcta tgctggcgtc
     3841 ttttatgaat aaccagcgac tggttcgcag cgtgggggaa cacctgacgg cttggcaccg
     3901 gatgcgcgga tattcatctg aatcgaaaaa aactgcactg caacgtcttc aggctgacag
     3961 ccagttactg aacgactggc agcaacgtgg tgtgccgcag cgttacggac tgggattgta
     4021 tcagggaatg tggctgatac cgccggttga gcgagcgact ggtgagtgga ttgcaccact
     4081 gcctccccgt ccggtaatac agaaagttac tcagacgccg aaaatggttc gccttaacag
     4141 cctggc
//
LOCUS       DAZKJR010000250         4051 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKJR010000250 DAZKJR010000000
VERSION     DAZKJR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.251"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(213..860)
                     /gene="ychE"
                     /locus_tag="ACHOWS_005031"
     CDS             complement(213..860)
                     /gene="ychE"
                     /locus_tag="ACHOWS_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFQ0050466.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(891..944)
                     /gene="ychE"
                     /locus_tag="ACHOWS_005032"
     CDS             complement(891..944)
                     /gene="ychE"
                     /locus_tag="ACHOWS_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFQ0050467.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1337..4012
                     /gene="adhE"
                     /locus_tag="ACHOWS_005033"
                     /gene_synonym="adhC"
     CDS             1337..4012
                     /gene="adhE"
                     /locus_tag="ACHOWS_005033"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0050468.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVAYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1121 a          998 c          948 g          984 t
ORIGIN      
        1 catttcaatt attcttcagc aagcccagga ttatgatgtg gcgaaaataa catgatggtg
       61 aggagaagca gttaaggaaa tccttataac taactgtaat ttgagaagaa tattttcacc
      121 cattcctttt gcttttgata actgcgcggg aacaaagtaa aaataatgac gaagtaaacg
      181 gagcatgaca gctccgtttc atttgaaagg aattaattaa gcaggccggg gaaaatcccc
      241 ttaataccag taacgataaa ttcaatcccc aatgccatca gcaatagccc cataatacgc
      301 gtaatcacgt tgatgccggt ctggcgtaaa acccgtacca gccacggtgc catgcggaac
      361 aatccccagc aacataaagc gaacaatgca atagccacaa agaaaccaaa cagatagcta
      421 atgctgtgat aacgcgtccc ccagacgatg gtagaactga tcgcccctgg ccccgccatc
      481 aacggcaacg ccaatggcac cacaccaatg ctttcacgca ccgcggtttc tgatttttct
      541 tgcttgttct gtttatcctc gccaagcttg ccgctgatca tcgacatcgc tatcgtcacc
      601 accaggatgc caccggcgat acggaatgaa tcaattgata taccaaaaag ttgtagaatc
      661 gtgtcgccga gaaaaagcga gatccacaag ataatggcca cagacaggtt ggctgtaagg
      721 ttcgttttgt ttcgcgctgc cgctgtctga taactggtca tgctgataaa gacgggaata
      781 atccctaccg ggttgaccag cgcaaataac ccgatgaaaa atttgaagta aacgggaaaa
      841 tcaaaaaggg tctgaatcac ggttagctcc gaagcaaaag ccggataatg ttagccataa
      901 ataaggttaa aaagacgcgc tgacaatacg ccttttgaca gcatttttca cctcctaact
      961 acttaaaatt gctatcattc gttattgtta tctggttgtg caaaacatgc taatgtagcc
     1021 aacaaatcat actacaattt attaactgtt agctataatg gcgaaaagcg atgctgaaag
     1081 gtgtcagctt tgcaaaaatt tgatttggat cacgtaatca gtacccagaa gtgagtaatc
     1141 ttgcttacgc cacctggaag tgacgcatta gagataataa ctctaatgtt taaactcttt
     1201 tagtaaatca cagtgagtgt gagcgcgagt aagcttttga ttttcatcgg ttaagcaaat
     1261 catcaccgca ctgactatac tctcgtattc gagcagatga tttactaaaa aagtttaaca
     1321 ttatcaggag agcattatgg ctgttactaa tgtcgctgaa cttaacgcac tcgtagagcg
     1381 tgtaaaaaaa gcccagcgtg aatatgccag tttcactcaa gagcaagtag acaaaatctt
     1441 ccgcgccgcc gctctggctg ctgcagatgc tcgaatccca ctcgcgaaaa tggccgttgc
     1501 cgaatccggc atgggtatcg tcgaagataa agtgatcaaa aaccactttg cttctgaata
     1561 tatctataac gcctataaag atgaaaaaac ctgtggtgtt ctgtctgaag acgacacttt
     1621 tggtaccatc actatcgctg aaccaatcgg tattatttgc ggtatcgttc cgaccactaa
     1681 cccgacttca actgctatct tcaaatcgct gatcagcctg aagacccgta acgccattat
     1741 cttctccccg cacccgcgtg caaaagatgc aaccaacaaa gcggctgata tcgttctgca
     1801 ggctgctatc gctgccggtg ctccgaaaga tctgatcggc tggatcgatc aaccttctgt
     1861 tgaactgtct aacgcactga tgcaccaccc agacatcaac ctgatcctcg cgactggtgg
     1921 tccgggcatg gttaaagccg catacagctc cggtaaacca gctattggtg taggcgcggg
     1981 taacacgcca gttgttatcg atgaaactgc tgatatcaaa cgtgcagttg catctgtgct
     2041 gatgtccaaa accttcgaca acggcgtaat ctgtgcttct gaacagtctg ttgttgttgt
     2101 tgactctgtt tatgacgcag tacgtgaacg tttcgcaacc cacggcggct atctgttgca
     2161 gggtaaagag ctgaaagctg ttcaggatgt tatcctgaaa aacggtgcgc tgaacgcggc
     2221 tatcgttggt cagccagcct ataaaattgc tgaactggca ggcttctctg taccagaaaa
     2281 taccaagatt ctgatcggtg aagtgaccgt tgttgatgaa agcgaaccgt tcgcacatga
     2341 aaaactgtcc ccgactctgg caatgtaccg tgctaaagat ttcgaagacg cggtagaaaa
     2401 agcagagaaa ctggttgcta tgggcggcat cggtcatacc tcttgcctgt acactgacca
     2461 ggataaccaa ccggctcgcg ttgcctactt cggtcagaaa atgaaaacgg cccgtattct
     2521 gatcaacacc ccggcgtctc agggtggtat cggtgacctg tataacttca aactcgcacc
     2581 ttccctgact ctgggttgtg gttcctgggg tggtaactcc atctctgaaa acgttggtcc
     2641 gaaacacctg atcaacaaga aaaccgttgc taagcgagct gaaaacatgt tgtggcacaa
     2701 acttccgaaa tctatctact tccgccgtgg ctctctgcca atcgcgctgg atgaagtgat
     2761 tactgatggc cacaaacgtg cgctcatcgt gactgaccgc ttcctgttca acaatggtta
     2821 tgctgatcag atcacttccg tactgaaagc agcaggcgtt gaaactgaag tcttcttcga
     2881 agtagaagct gacccgaccc tgagcatcgt tcgtaaaggt gcagaactgg caaactcctt
     2941 caaaccagac gtgattatcg cgctgggtgg tggttccccg atggatgccg cgaagatcat
     3001 gtgggtgatg tacgagcatc cagaaactca cttcgaagaa ctggcgctgc gctttatgga
     3061 tatccgtaaa cgtatctaca agttcccgaa aatgggcgtg aaagcgaaaa tgatcgctgt
     3121 caccaccact tctggtacag gttctgaagt cactccgttt gcggttgtaa ctgacgacgc
     3181 tactggtcag aaatatccgc tggcagacta tgcgctgacc ccggatatgg cgattgtcga
     3241 tgccaacctg gttatggaca tgccgaagtc cctgtgtgct ttcggtggcc tggacgcagt
     3301 aactcacgcc atggaagctt atgtttctgt actggcatct gagttctctg atggtcaggc
     3361 actgcaggca ctgaaactgc tgaaagaata tctgccagcg tcctaccacg aaggatctaa
     3421 aaatccggta gcgcgtgaac gtgttcacag tgcagcaact atcgcgggta tcgcgtttgc
     3481 gaacgccttc ctgggtgtat gtcactcaat ggcgcacaaa ctgggttccc agttccatat
     3541 tccgcacggt ctggcaaacg ccctgctgat ttgtaacgtt attcgctaca acgcgaatga
     3601 caacccgacc aagcagactg cattcagcca gtatgaccgt ccgcaggctc gccgtcgtta
     3661 tgctgaaatt gctgaccacc tgggtctgag cgcaccgggc gaccgtactg ctgctaagat
     3721 cgagaaactg ctggcatggc tggaaacgct gaaagctgaa ctgggtattc cgaaatctat
     3781 ccgtgaagct ggcgttcagg aagcagactt cctggcgaac gtggataaac tgtctgaaga
     3841 tgcattcgat gaccagtgca ccggcgctaa cccgcgttac ccgctgatct ccgagctgaa
     3901 acagattctg ctggatactt actacggtcg tgattatgta gaaggcgaaa ctgcagcgaa
     3961 gaaagaagcc gctccggcta aagctgagaa aaaagcgaaa aaatccgctt aatcagtagc
     4021 gctgtctggc aacataaacg gccccttctg g
//
LOCUS       DAZKJR010000251         4048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKJR010000251 DAZKJR010000000
VERSION     DAZKJR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.252"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..148
                     /locus_tag="ACHOWS_005034"
     CDS             <1..148
                     /locus_tag="ACHOWS_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050469.1"
                     /translation="NLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYMSI
                     NTHF"
     gene            258..1606
                     /locus_tag="ACHOWS_005035"
     CDS             join(258..738,738..1606)
                     /locus_tag="ACHOWS_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0050470.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNMVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIRSLKCGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHNDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAVMEN
                     FLGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPLEYRTEYQKAG"
     gene            1760..2380
                     /gene="entD"
                     /locus_tag="ACHOWS_005036"
     CDS             1760..2380
                     /gene="entD"
                     /locus_tag="ACHOWS_005036"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFQ0050471.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFEPASFHEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAVHALREFGFKYAPPVGERRQPLWPAGLYGSISHCQATALAVVSHQ
                     PTGIDIEEIFSAQAAEELKDSIMTPAEYELLVVSNLPFSVVLTLAFSAKESAFKASKL
                     QTDQGFLDYQIVNWNKQRIIICWENEIFAVQWQIRGKQLITLCQHD"
     gene            2724..3659
                     /locus_tag="ACHOWS_005037"
                     /pseudo
     CDS             2724..3659
                     /locus_tag="ACHOWS_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3807..>4048)
                     /locus_tag="ACHOWS_005038"
     CDS             complement(3807..>4048)
                     /locus_tag="ACHOWS_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein acyltransferase"
                     /protein_id="HFQ0050472.1"
                     /translation="YVHNGVVITQSDYIRLNNGRGYKTTLVNGSLLAKFSVAPNTKLI
                     NVQNYGGTIVFNNDWEIDWDIYNKSKKEHFFVVTL"
BASE COUNT         1145 a          926 c          987 g          990 t
ORIGIN      
        1 caacctgttc gacaaacgtt tgtggcgtgc gggtaatgcc cagaccacgg gcgatctggc
       61 aggagccaac tatatcgccg gtgccggggc gtatacctat aacgagccgg gacgtacgtg
      121 gtatatgagc attaatactc acttctgatg ctaacgtcag attgttgaca aagtgcgcgt
      181 aattcatgcc ggatgcggcg tgaaactgaa ctgcacccca aatgttggac ataatctgac
      241 atctgaaggt gcagtttatg aaacattcat ttgaagtaaa acttgccgct gtaaatcatt
      301 acctggctgg tcatgcaggc atcatttcta cggcaaaact ctttcaactt tctcacacca
      361 gcctctcaca ctggattaat ctttttcttc ttcacggtcc tcgggcactg gattgcagac
      421 acaagcgtag ctattctcct gaagataaac tttgcgtggt tctttatgct ctcgggcatt
      481 ctgagtctct accccgggta gctgcccggt ttaacattcc cagccacaac atggttaaaa
      541 actggataaa aggctaccgc aaatctggta atgaggcctt tatcaggcgc aggaaggaga
      601 aaagcatgac ccgttctgat gatacccatg aaaacgaggc aaacatgacg ccggaagaga
      661 tgaagaatga gctgcgttat cttcgcgcag aaaatgcata tctgaaagcc atgcaggagc
      721 atctcctgga aaaaaagcgc caggagctgg agaaaaaacg aaagtcatcc ggagcctgaa
      781 gtgcggacac tgccagtctg acctgttaaa ggctgcaggg ctggccagaa gcacgctgta
      841 ctaccagctg tcactgcaga aggctaagga caagtatgct gatgtgaaac agctcatagc
      901 ctccatcttc catgaacacc ggggatgtta tgggtaccgg cggatccatt gtgagcttca
      961 gaaaagaggt cttaagttca gtggcaaaac ggtgcgaaaa ctgatgcaac agctcggcct
     1021 gaagtctcct gtgcgcctga agaaataccg gtcttaccgg ggaaatatgg gactcgctgc
     1081 agaaaatatc cttcagcggc agttcaaagc ggaagctccc tgtgagaaat gggtgacaga
     1141 tatcaccgaa ttcagggctg gcggacaaaa actgtacctg tccccgatac ttgatttgtt
     1201 taacggggaa atcgtggcgt gggaaactgc ctgcaggcct acagaggagc tggtgaagcg
     1261 catgttaaat aaggggctgg aaagccttgc agaaggagaa aaaccgctgc tccacaatga
     1321 tcaagggtgg cattaccgga taaaaagtta tcagtctgcc ctggcagaca aagggttagt
     1381 gcagagcatg tcacgcaagg gcaactgtct tgataatgcg gtaatggaga atttcttagg
     1441 tcacctgaaa gaagaaatgt actatcgccg ggactacaga agtgtagaag agctggaaaa
     1501 tgccgttaac gaatacataa cttactggaa tcaaaaaaga ataaaactca gtcttggggg
     1561 actcagcccg ttagaatacc ggactgagta tcaaaaagcc ggttaactga aactgtccag
     1621 gttttggggt tcagttcaga acgccttatc cggcctacaa aaatcttgcc aattcaatat
     1681 attgcaggat agtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcatc
     1741 agtctctaat atggtcgata tgaaaactac gcatacctcc ctcccctttg ccggacatac
     1801 gctgcatttt gttgagttcg agccagcaag ttttcacgaa caggatttac tctggctgcc
     1861 gcattatgcg caactgcaac atgccggacg gaaacgcaaa gccgaacatt tagccggacg
     1921 gattgctgct gttcatgctt tacgggaatt tggctttaag tacgcccccc ctgtcggtga
     1981 acgtcgccag ccactttggc ctgccgggtt atacggcagt attagtcact gccaggctac
     2041 ggcattagcg gtggtatccc atcaaccaac gggtattgat atcgaagaga tcttctctgc
     2101 acaagcagcg gaagagttga aagacagcat tatgacacca gcagaatacg agctgcttgt
     2161 ggtcagcaat ttaccctttt ctgtggtgct tacactggcg ttttcagcga aagagagtgc
     2221 gtttaaggca agcaagttac agacagatca aggttttctg gattatcaga tagtgaactg
     2281 gaataaacag cggatcatta tttgttggga gaacgagata tttgctgtgc aatggcagat
     2341 aagaggaaaa caactcataa cgctttgtca acacgattaa tggataaccc ttactgtggt
     2401 tagccagcaa ccattgcttg tagtagtcaa gtaatattga ccaccgtttt acgctaagaa
     2461 cgttatctgt tctctaattt tttcggtgtc agcccaccgt tataatggtg agatctgacg
     2521 ctattatagt tgttcaatat acaaccactg atttgctgcc atatatccgg cgtcgttagt
     2581 ggactgatga cccgcgccat gatggcatcc gcaccctgtt tcctaaccta tgttaccacc
     2641 ccgacaccgt tcaggttgtt acagagtcga ttatgcgatc tgatcaatcg ctgaatatcc
     2701 caaatcacca accggacgga gcagtgccga tcatagcacc aatacccaga actgaacgac
     2761 gcctgatcca gaaaacgatc cataaaacaa aggacaaaaa accacgcccg gcggctcaca
     2821 gccatgctga tgctgcatcg tggtgacacc gtcagtcatg ctgccagaac actctgctgt
     2881 gctcgctcat cgattggtcg ctggattaac tggttcacgc tgtcgggtgt tgaaggcctg
     2941 aagtcattgc cgtcaggacg tgagaggcgc tggccgttcg agcatatttg cgcattgctg
     3001 cgtgagcttg ttaagcattc tcccggcgat ggttacctgc agccgaactg gtatggcgca
     3061 gagcagcacc aacactgcgt atccggggcc cgaataagga agaaaaaatg gcagcgatta
     3121 atgtagcgtt agtcagttgt agcatagaac accctgtgtt ttacgaagat gaagtggaga
     3181 tccacctcaa tcccaaaatc ggcgcagact ggcagttacg cgggcagcaa aagcgcgttg
     3241 ttacgccggg ccggaatgaa aagtactatc ttgccggtgc gctgcacagt ggtacgggca
     3301 aagtcagcta cgtgggtgga aacagtaaag gttcatcctt atttatcagg ctgttaaaac
     3361 atctgaagga gacatactga cgggcaaaaa agattacgct cattgtcgat aactacatca
     3421 cccacaaaag ctgtgaaacg ttgcgctggc tgaaagcaaa cccaaagttc agggtgattt
     3481 atcagccaat ttactcgcca tgggtcaatc atgtcgaacg gttgtggcag gcgcttcatg
     3541 aaacaataac ccgaaatcac cagtgccgtt caatgtggca actgttgaaa aaggttcgtc
     3601 actttatgga taccgtcagc ccattccccg gtggaaagca tgggctggct aaagtgtagc
     3661 ggtattagga tcagctactt agaagcgaat ggagtaactg gcatagcaca ggggaattta
     3721 actactcagc atatatatgc aagacttgaa taatatgtgg gcgcgtaaat agctcataaa
     3781 aatttcaaca acactccctc gctgtattat aaagtaacca caaaaaaatg ttcttttttg
     3841 gatttgttat aaatatccca gtcaatttcc caatcattat taaaaacaat agttccacca
     3901 taattctgga catttattaa cttggtgttt ggtgcaacgg aaaactttgc aagtaagcta
     3961 ccgttaacca atgtagtctt atatccccta ccgttattaa gtcttatgta atcagattgt
     4021 gttattacga ccccgttgtg tacatatt
//
LOCUS       DAZKJR010000252         3979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKJR010000252 DAZKJR010000000
VERSION     DAZKJR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.253"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..972
                     /locus_tag="ACHOWS_005039"
     CDS             <1..972
                     /locus_tag="ACHOWS_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0050473.1"
                     /translation="HEKETLEYKRQLAELGDKVEYQKRLNELAQQAVRFEEQQSAKQA
                     AISAKARGLTDRQAQRESEEQRLRDVYGDNPQALAQVTGALKQTWADEDMLRGDWLAG
                     LKSGWGEWTESATDSFSQVKSAATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTE
                     ILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIEAAAANFHFATGGFTGTGGKYEPA
                     GIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNH
                     VVIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
     gene            969..1298
                     /locus_tag="ACHOWS_005040"
     CDS             969..1298
                     /locus_tag="ACHOWS_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0050474.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            1298..1996
                     /locus_tag="ACHOWS_005041"
     CDS             1298..1996
                     /locus_tag="ACHOWS_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFQ0050475.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            2007..2750
                     /locus_tag="ACHOWS_005042"
     CDS             2007..2750
                     /locus_tag="ACHOWS_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0050476.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCMNISAEPE
                     AYFRIAPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCCFGSSVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            2747..3328
                     /locus_tag="ACHOWS_005043"
     CDS             2747..3328
                     /locus_tag="ACHOWS_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFQ0050477.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAC
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          901 a          903 c         1339 g          836 t
ORIGIN      
        1 catgagaaag agacgctgga gtacaaacgt cagctggctg agctgggcga caaggttgaa
       61 taccagaaac ggctgaatga gctggcacag caggcggtgc ggtttgaaga gcagcagagc
      121 gcgaagcagg ccgccatcag cgcaaaagcc cgtggtctca ctgaccgtca ggcacagcgg
      181 gagtctgaag agcagcgtct tcgtgacgtg tacggcgata atccgcaggc gctggcacag
      241 gtcaccgggg cactgaaaca gacatgggcg gatgaagaca tgctgcgcgg tgactggctg
      301 gccgggctga agtccggctg gggggagtgg acggaaagtg cgacggacag tttttcgcag
      361 gttaaaagtg ctgccacgca gacctttgac ggtattgcac agaatatggc ggcgatgctg
      421 accggtgcag aggcagactg gcggggattc acccgttcgg tgctgtccat gatgacagaa
      481 atcctgctta aacaggccat ggtgggaatt gtcgggcgta tcggcagcgc cattggcggt
      541 gctttcggtg gtggtgcatc tgcttcctcg gggacggcca ttgaggctgc ggcggcgaac
      601 ttccatttcg cgaccggagg atttacgggg acgggcggca aatatgagcc tgcggggata
      661 gttcaccgcg gggagtttgt tttcacgaag gaggcaacca gccggatagg tgtggggaat
      721 ctttaccgtc tgatgcgcgg ctatgcggaa ggggggtatg tgggtggtgc cggaagtccg
      781 gcgcagatgc ggcgggcgga aggtattaat tttaatcaga acaatcacgt ggtgattcag
      841 aacgacggta tcaacggaca ggcggggccg cagctgatga aggcggtgta tgacatggcc
      901 cgtaaggggg cgcaggatga actccggctg cagttgcgtg atggcggtat gttatcaggg
      961 agcgggcgat gaaaaccttt cgctggaaag tgaagccgga tatggaggtg aactcgcagc
     1021 catcggtgcg tgaagtgcgt tttggtgacg ggtactcaca gcgtatggcg gcagggctga
     1081 atgctgacct gaaaacatac agggtgatgc tttccgtgac ccgggaggag gcccggcatc
     1141 tggaagcgtt tctggcagag cacgggggct ggaaggcatt tttgtggaag ccaccctatg
     1201 cataccggca gataaaggtg acctgtgccg ggtggtctgc gcgggtcggg atgttgcgcg
     1261 ttgagttcag cgcggagttt aagcaggtgg tgaactgatg caggatattc acgaagcaag
     1321 cctgaacgag tcggttaagt cagagcagtc accgcgggtg gtgctctggg aaatcgacct
     1381 gacggtgcag ggcggtgagc ggtatttttt ctgcaatgag ctgaatgaaa aaggggagcc
     1441 ggtgacctgg caggggcgtg aatatcaggc gtacccgatt gagggcagcg gctttgagat
     1501 gaacggaaag ggcagcagtg cccgcccgtc gctgacggtg tccaatctgt ttggccttgt
     1561 caccgggatg gcggaggatt tgcagagcct ggtgggtgcc acggtggtcc gccgccgggt
     1621 gtatgcgcgt tttctggatg cggtgaactt tgtggcaggg aatccggagg cagacccgga
     1681 gcaggagctg acggaccggt gggtggtgga gcagttatca gcgctgacgg ccatgacggc
     1741 ctcgtttgtg ctggcgacac cgacggagac ggacggtgcg ctgtttcccg gtcgcatcat
     1801 gctggcgaac acctgtatgt gggattaccg gggcgatgaa tgcgggtata acggtccggc
     1861 agtggcggat gagttcgaca accccaccac ggatatccgg aaggacagat gcagtaaatg
     1921 catgcgcggg tgtgagatgc gcggcatggc ggtcaatttt ggcggtttcc tttccatcaa
     1981 taaactttcg cagtaaatcc cgttttatga cacagactga atcagcgatt ctggtgcatg
     2041 cccggcggtg tgcgccagcg gagtcgtgcg gcttcgtgat aagcaccccg gagggcgaac
     2101 ggtaccagcc ctgcatgaat atctccgcag agccggaggc gtattttcgt attgcaccgg
     2161 aagactggct gcaggcacag atgcaggggg agattgtggc gctggtccac agtcatcccg
     2221 gtggtctgcc ctggctgagc gaggcggacc ggaggctgca gataaagagt gccctgccct
     2281 ggtggctggt ctgccggggt gaaattcacc ggttccgctg tgtgccgcac ctgaccggac
     2341 ggcgctttga acacggtgtg acggactgtt acaccctgtt ccgggatgca taccatctgg
     2401 cggggataac gctgccggat tttgtgcgtg aggatgactg gtggcgcaac ggccagaacc
     2461 tgtacctgga caacctggcg gaaaacggct tttaccgggt gtctccgtcc tgtgcacagg
     2521 caggcgatat cctgctgtgc tgctttggtt catcggtgcc gaatcatgcc gccatttact
     2581 gtggcaacgg tgaactgctt caccatatac ctgaacaact gagtaaacgg gagaggtatt
     2641 ctgaaaaatg gcaacgacga acgcattctg tctggcgtca ccgccactgg tccgcatctg
     2701 ccttcacggg gatttacaac gatttggtcg ccgcatcagc ctgtatgtga acacggcagc
     2761 ggaggccatc cgtgccctgt cgctgcaggt gccgggattc cgctgtcaga tgaacgaagg
     2821 ctggtatcag atacgtattg ccggtgagga taccgcgccg gaggcggtgt atgcctgtct
     2881 tcacgaacaa ctgggtgagg gagcggtcat ccacattgtg ccgcgactgg ccggagccgg
     2941 gggaaatggt gtttttcagg tggtgctggg ggttgcagcg attgtcgggt catttttcac
     3001 tggtggtgca acactggcgg catggggcgc agccctgagt gccggagggc tgactgccac
     3061 cacgatgctg ttctcactgg gtgccagcat gatactgggt ggtgtggccc agatgctggc
     3121 cccgaaggca aagacgccgg agtacaaaag tacggataac ggtaaacaga acacgtattt
     3181 ttcgtcactg gacaacatga ttgcccaggg taacccgatg ccggtgcctt acggtgaaat
     3241 gctggttggt tcacgacgga tatcccagga catcagcacc cgtgatgagg gcggagacgg
     3301 gaaagtggtg gttatcgggc ggggataaaa ataaaaaaaa tcccgcagag ttagcggagc
     3361 tgcgggaaat aacgatgaag attaacgtta tggagttatt tttttgggca gaaaaacatt
     3421 aacgcagcga aattataagc gctacatgga ttttgcgaaa atgtgaagaa attcagaaat
     3481 tttttgctgc agaggtgcag ggagcttgcc gggtatttgt ggcagaagac agaaaacatc
     3541 atgccggggt taagggataa aaaaatcccg cagagtccgg agactgcggg acagaaccga
     3601 tgaagattaa cgttcaagga gttttgtttt tattgacccg aaaaaactgt aacgccccgg
     3661 aatgatatct gccacgggga cgtacagaaa atgtgaagaa attcagactt tttctgtcgc
     3721 aggtttacag aacggatgac cgccagggta ccagtggcgt aaatgagcag gatgttgtgg
     3781 tgtggctgaa gcaaaaaaat ctcccgtaat atcgccggat actgacggga gacgaatagt
     3841 caccttaagg aggtctgata attattgctt ttattgagca aaaaaactgt aacgctgcgt
     3901 cattatagtg gctacaggca attgccggaa atgtgaagaa tttcagaaat tttattccgt
     3961 catgacacag gcaccctcc
//
LOCUS       DAZKJR010000253         3963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKJR010000253 DAZKJR010000000
VERSION     DAZKJR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.254"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1357
                     /locus_tag="ACHOWS_005044"
     CDS             <1..1357
                     /locus_tag="ACHOWS_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0050478.1"
                     /translation="AVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSMDVEPGLYR
                     VTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAVAAEILKRA
                     EASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGETGPQGLQG
                     VKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAATPKAVKAAMDKADGCL
                     EKAKNGDDIPDKVKFLNTVGAARVYGRDIHTETGEWTTSEFVAWLKEKGAFDQPYWMM
                     KASLLAEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVRVTIGEYGGDGFLNGTVCTC
                     TVYGDTQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLRQWGSIEVSEDDGKLMTQ
                     HSIRFPVAFPVAALNAQVSAVGDPNAIKMYTLSNPSLVSVTLITVRGCYGKFSWEAIG
                     Y"
     gene            1367..1591
                     /locus_tag="ACHOWS_005045"
     CDS             1367..1591
                     /locus_tag="ACHOWS_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0050479.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            1591..2262
                     /locus_tag="ACHOWS_005046"
     CDS             1591..2262
                     /locus_tag="ACHOWS_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFQ0050480.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPENRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILSYRV
                     GWAQE"
     gene            2637..3788
                     /locus_tag="ACHOWS_005047"
     CDS             2637..3788
                     /locus_tag="ACHOWS_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFQ0050481.1"
                     /translation="MDKSLSRSVISVVIGGILFGVSAGVLAQDAAENKLIASGSIPEN
                     TDLSASARADGEIALAVGTGASADKKFATSVGSWSAARGQQSVALGGATYAYADASTA
                     LGSVAFVDSTATYGTAAGNRAKVDKDATEGTALGAKATVTNKNSVALGANSRTTRDNE
                     VYIGYEAESGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQDTNTRLVVEA
                     KKSREYTDSRTTVGVNSDGTLTRAEGASKTLAVNDGLVALSGRTDRIDATVGSVDRRV
                     TKNTQAIQSTSRQLQEHNTRLNSHQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFN
                     ATAAVGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSWNAGVNFEF"
BASE COUNT         1051 a          793 c         1251 g          868 t
ORIGIN      
        1 agcggtaccg ggatgcacga tagagctgaa agcgcgacgc acaacggaga cggtgattgt
       61 caccacggtg gcgtatggtc agccggggga aaccggcagt tacagtatgg atgttgagcc
      121 ggggttgtac cgggtgacgc tgaacacgga agggtacgcg ccgtcatatg tgggtgacat
      181 tctggtgaag gcggattctg caccgggaac gctgaataaa tttctgatgg acctggaaga
      241 cgcacagtat tacccgaagg cccttgcaga gctggaagcg gtggcagcgg aaatactgaa
      301 acgtgcggaa gcgtccgcag ccagtgcgga ggaggcaaaa aaacgggcag agaatgcgcg
      361 gggaccgaag ggggataagg gggacaccgg gccgcagggt attcccgggc caaaaggcga
      421 taccggcgag cgtgggccaa agggtgagcg tggtgagaca ggaccacagg ggcttcaggg
      481 tgtgaaaggt gaacggggag agaagggcga aaaaggcgag ccgggagggc cggatgcgac
      541 gacggcacag aagggaattg tgcagttaag cagcgcaacg gacagtgatg atgaaacgaa
      601 ggcagccacc ccgaaagcgg tgaaagcggc aatggacaaa gcggacggat gcctggagaa
      661 agcgaaaaac ggtgacgata tcccggataa ggtgaagttt ctgaacaccg tgggagcagc
      721 cagagtatac gggcgggaca ttcatacgga gaccggtgaa tggaccacga gtgagtttgt
      781 ggcctggctg aaagaaaagg gggcatttga ccagccttac tggatgatga aggcatcact
      841 gcttgcggaa tttaataagg tcatcacgga tgtcggaccg ggaaaactca atctgggagg
      901 ctgcgccatt gaggtgatgg ggacgtataa cgcagccata gtccgggtca ccatcggcga
      961 atacggtggc gatggttttc tgaacggcac ggtctgtacc tgtacagttt acggagacac
     1021 acaacgtttt cactggcggg tggattacag cacaaaaaac aagcccacaa cagcaagcct
     1081 gacagtaaat ggctgggagc gtgatgaagt aaccggtcgt ttgcggcaat ggggcagtat
     1141 tgaggtaagt gaggatgatg gtaagcttat gacacaacac tctatccgtt ttcctgtcgc
     1201 ttttccggtt gctgctctga atgctcaggt gtctgcagtg ggagacccca acgccattaa
     1261 gatgtatacc ctgtcgaatc cgtcattagt gtctgtgact ctgataacag tcaggggatg
     1321 ctacggcaag ttttcatggg aagcgatcgg atactgaggg cattgtatgg aacagtatgt
     1381 tttttcaccg tcagagaata tgttctatcc cctgtccctg cggccggttt atgaggcagc
     1441 agggcgctgg ccggaagacg gtattgtggt ggattatgtg gtgtataaag tctttgcagc
     1501 ggatgctgca ccggcaggta tgaagcgggg cgtcggtgcg gaaaaaatgc cggtctgggt
     1561 gccggtatcg gaggagggca cggaaacatg atgcatatca gaaatttttc gtattacacc
     1621 ccggcggagc cggatgttgc aggcgcgatg tacctgaagt ctgaggatgg tcaggactgg
     1681 tatgagtgtc aggcgttgtt ttcgccggag acgctgaagg tggtgtatga cagccggggt
     1741 gtgattacgg gatacggtaa ggacactgcg ttactgtggc cggtgaacca gagtgtggca
     1801 gaagtgccgg ataccccgga gaaccggaaa attgacctga gcgggcgctg gggttttgac
     1861 ggggagaaaa tcacggacct gctgaccgcg gagaaagcac gagggatgaa gggcgatgaa
     1921 attaacgcct ggcgtaatgc gatggaagcg gcgaactaca cgtttgagca caatgggcgt
     1981 aaatgggact acgggaagtc aacgcagacg cgtcttgagc catcggtggc ggcggcgaaa
     2041 gcggggaaac tgccggaggc gtttttctgg acggatgcgg aaaacaatga tgtggaggtg
     2101 acagcagaag agcttatagc gctgagtgaa gcggcagagc aggcgatgtt caccaaaggg
     2161 atggaaatcc acgtccggca gcgcaccatg aagaaagagc tggaaaagct gaccagtgcg
     2221 gatgagatac tgtcatacag ggttggctgg gcacaggagt aattggtgga aaagagggat
     2281 tgtccctttc tgtttttact gaaagagtcg taataattac gcagggaaat atcatggctg
     2341 aactaaacag ttaaattttg atgatattgt gatgttgctc ataaattaca gtgtaagcag
     2401 atgtgaaata tccagcggca tgagatcagt tttttattga gcattgctct gccttgtgct
     2461 ccagtatttg gagtaagggt gtaaaaaata cactaagata tttcctaaaa attaaaatta
     2521 aatttgtggg attttttagg aatatcctca tgggtgccga aatacatcag attttcttta
     2581 ttcatctgat gtatttcggt ttgtttgtat acaaataaat tcatgaggaa tcatacatgg
     2641 ataagtcttt atcacgaagt gttatttccg ttgttattgg cggtatttta tttggtgtaa
     2701 gcgctggcgt tctggctcag gatgcagcgg agaataagct tatcgcctca gggagtatcc
     2761 cagaaaatac agatctttct gcttcagctc gcgcggacgg agagattgcg ctagctgttg
     2821 gtacaggggc cagcgcggat aaaaaatttg caacatcagt gggatcatgg tctgcagcac
     2881 gaggacagca aagtgtggct ttaggtgggg caacatatgc ttatgcagat gcatctacag
     2941 ccctgggaag cgttgcattt gttgatagca cggctacata tggcacggca gcaggtaacc
     3001 gagccaaagt tgataaagat gcaacagaag gtactgcgtt aggggcaaag gcaacagtaa
     3061 caaataaaaa cagtgttgca ttaggagcta attcacgcac aacccgtgac aatgaggttt
     3121 atatcggtta tgaagcagag tcgggtaagg cttataaaac ccgggtgctt ggtggtctga
     3181 gtgacggtac tcgtccctca gatgctgcaa ccgttcgtca ggttgaccgt gtaaaagaca
     3241 gtgttgagca actggctcag gatacgaata cccgccttgt ggttgaagcg aaaaagtctc
     3301 gtgaatatac agactccaga acgacagttg gtgttaactc cgacggaacc ctgacccgtg
     3361 cagaaggagc cagcaaaacc ctggctgtta atgatgggct tgtagctttg tccggcagaa
     3421 cagaccgtat tgatgctact gttggtagtg ttgaccgccg ggttactaaa aatacacagg
     3481 cgattcagtc cacttcccgt cagttgcagg agcacaacac acgtctgaac agtcatcagc
     3541 gtcagattaa tgaaaaccac gaagagatga aacgtgcggc agcccagagc gcagcgctgg
     3601 caggtctttt ccagccgtac agtgtgggta aattcaacgc cactgctgcc gttggtggtt
     3661 acagtgataa acaggctgtt gccgtgggtg ttggttaccg tttcaatgag cagacagcag
     3721 cgaaagcagg tgtggcagcg agcgatggcg atgtatcctg gaacgctggt gtgaactttg
     3781 agttttaatt gctgacagtg acatgaagca tgacggcagg cgtggagttt tgcctgccgt
     3841 tttttctcat gtgagcatga aacaggtgac agcgatgaaa gtgattctgg cgacccgcaa
     3901 ccgttatctg gagtatggcc tgcagcagat gcttgagggg tacaggataa tactggcgag
     3961 gga
//
LOCUS       DAZKJR010000254         3843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKJR010000254 DAZKJR010000000
VERSION     DAZKJR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.255"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..574)
                     /gene="ogt"
                     /locus_tag="ACHOWS_005048"
     CDS             complement(59..574)
                     /gene="ogt"
                     /locus_tag="ACHOWS_005048"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0050482.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAANPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(585..728)
                     /locus_tag="ACHOWS_005049"
                     /pseudo
     CDS             complement(585..728)
                     /locus_tag="ACHOWS_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            728..944
                     /locus_tag="ACHOWS_005050"
                     /pseudo
     CDS             728..944
                     /locus_tag="ACHOWS_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1118..1933)
                     /locus_tag="ACHOWS_005051"
     CDS             complement(1118..1933)
                     /locus_tag="ACHOWS_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0050483.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            2030..3058
                     /locus_tag="ACHOWS_005052"
     CDS             2030..3058
                     /locus_tag="ACHOWS_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0050484.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSRKANLES
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLVQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            3055..>3843
                     /locus_tag="ACHOWS_005053"
     CDS             3055..>3843
                     /locus_tag="ACHOWS_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFQ0050485.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGSAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDK"
BASE COUNT         1022 a          836 c          922 g         1063 t
ORIGIN      
        1 gttgttaagg gtaactttac ataaaagtgt gaacaagctg gcacaaattg tttaatgttt
       61 acagcaaaag ataaccttcg tggcgcaata accactcttt tcgctgaact ccgcctgcat
      121 atccggtcat ggtgccgttt cggccaataa cccgatggca aggtacaaca atgctgatgg
      181 gattcgatcc gttagccgca ccaacggcac gcgccgcgcc agggcgaccc aattgttcgg
      241 ccagttgacc gtaatgcatt acctgcccgc aggggatagt gcgtagtgtt ttccagactt
      301 cgcgctgaaa tggcgtcccc cccgtggcag tgggaagcgt atcaataatg ctaagattac
      361 cggcaaaata ttcacgaagc ttgtcgctta aaccacctgg gttggcggca gaaatgcgct
      421 catagccttc tttgcgataa tggatgtcca gcagctgcac catgcgttcg ctgtactctt
      481 cccattcaac cgcccgcagg cgaaattgct catcgcaaat cacccatagt ggacccagtg
      541 gcgtggcaat tttttcttca agtaatctca gcatccgttc tctcttaaga caaacgtggg
      601 taaatccccg gacctatcgg cagaccaaca agataccacg ccagcaacat cagcagccat
      661 accaccaaaa agataagcgg atagggcaag actaacgaat agtaagtacc cagtttcgcg
      721 tctggtttgt cgcagtctga tagcataaag cgattttttg atttttctgc ctgaagaaat
      781 gagggcaagc catcacatgg atcgagggtg ggataattcc agtcagcgaa attttaccgt
      841 aggctatctg ttatttgatt ctgtggcgtg atagccgcca gacaccactg atctcgtcac
      901 tcattcccca atcccggcga gaatgtggtt atttacagag ctaaaaaatg aataaaaaaa
      961 tagagcctgt cgacatccgc cagactctac agtacacaca gcagtgcatc ctcgtcttaa
     1021 atccggcctg gggtcctggt gaccggtgac agtgttataa cggttttacc catccgttgc
     1081 caattttctt cagtgacaga tttcacgaaa atataagtta aatcatttgc cttgtgattg
     1141 cataatcatg catcgttaat cgacgagtaa atttaccggg cgagtagccc gttcgacgct
     1201 taaacatatt gccgaacgat tcagctgagg catagcccag acgatgggca attttggtca
     1261 acgaatatcc atcatcaaga agcgtcactg cagaaataac gtgcatttgc tgaacccagt
     1321 ggcgaaatgt taatcccgtc tctttaacaa ataagcgcat gagtgtacgg ctactgacgt
     1381 taattttatc cgctagatta tccagtgttg gcagataccc ttggttgttt agcaaattct
     1441 cacaaagctg ctgtagccgc tcatcatttg gccaagggag ttcaagtgac attttcaccc
     1501 ccatttttat ttcctgaagt atcagtgtct cgagggctga aaaacgtaac tgttgtttat
     1561 ctgtggtact ttgcgacggt ggaagttggt taattgcaat tagtaattct cttaacaggg
     1621 gtgaaatacc cgtcatgaag caaacttttc ccagcgtttc actttccgct tctgaaacca
     1681 gtacggtttt taaaagtacg ttgcctgtcg caacaaactt atgcggcatt tgtgggggca
     1741 gccaaagcgc gctgctggca tgcactagcc agacgctttc tccagcccat acacgaacga
     1801 ctccttcagc cgggtaaagc aactgggcat agagatgttt atgccaggga actgtgcctc
     1861 cattcagatg ttccgaatcc agtccgataa tgtgatgccc tggtgatgct ttgggttgca
     1921 gtgtgattgt catttacagt aggtattttg tcagaaatca tcaaataagc catgttaaat
     1981 tttaaatatg gcgaatgcaa gtttccggat atatacggtg taaagagtga tgataatttc
     2041 taaaaaacag cttattgggg tagtagcgat cggtattatt ttggctggcg ttgtcttttt
     2101 tatctggtgg gtaagcaaag gaagatttat acagacgacg gatgatgctt acattggtgg
     2161 taatattacc acggtggcaa gtaaagtttc tggttatatt tctgctatag aggtgcgtga
     2221 taatcagagc gtaaaaaaag gcgatattat cctgcgtctc gatgatcggg attatcgtgc
     2281 aaatgtcgcc aggcttgagg caaaaataaa aagtagaaaa gccaatctcg aaagcattca
     2341 agccactatt acgatgcagc agtcgataat tcaaagtgcc agtgaaacat ggcaggcagt
     2401 aaaacatgag gagcaaaagc gcctgcgaga tactgaacgt tatgaaaaac tcgtacaatc
     2461 agcggcaata tcgcagcaaa ttattgataa cgccaggttt gactaccagc aggtcgcagc
     2521 aaaggagcgc aaagccgcga acgatttcct ggtggagaag caacggctgg cagttttatc
     2581 tgctcaggaa gaaaacgtac gtgcgagtat cgaagaagtt caggccgctt tgactcaggc
     2641 actactggat ctggaatata cccttgttcg tgcgccgatt gatggcattg tcgcaaaccg
     2701 cagcgcccat acaggcagtt gggtggaagg cggcacatcg ttagtttcac ttgtaccagt
     2761 cagtgagttg tgggttgatg caaattataa agaaaaccaa atagcgggaa tgaagccagg
     2821 aatgaaggct gagatccgtg ctgatattct caaaggggaa gtgtttcacg gtcacattga
     2881 aagcctttcc ccagcgacag gagccagttt cagcttgatt ccgatagaaa atgccactgg
     2941 caatttcacc aaaattgttc agcgtgtccc ggtacggatt gcatttgacg acgcaaagga
     3001 attaaagcaa cttcttcgcc cgggactgtc cgtcaccgtc agtgtcgatg agagatgatg
     3061 tctatgcgta aacatattgc tttcgccagt atgtgtatgg gattatttat tgctcagctt
     3121 gatattcaaa ttgtatcgtc atccctaaat gaaataggtg gtggtctgtc cgcaggtaaa
     3181 gacgaaatgg cctggcttca gaccagctat ttaattgctg agattattgt tattccgctc
     3241 tctgggtggc tgtcacgggt attttccact cgttggcttt ttactctatc cgcgggaata
     3301 tttactttaa tgagtatcgc gtgtggactt gcatggaata ttcagatcat gattttgttt
     3361 cgtgctttac agggagcggc tggcgcatcc atgatccctt tagttttcac gacggcgttt
     3421 atttattacc agggtaaaga gttggggtcg gctgctgcgg tagtcagtgc gctggcctca
     3481 ctgtcaccga cgctagggcc aactctgggg ggctggatca ctgataacct cgactggcga
     3541 tggctatttt atatcaatat attacctggc atatatttag ttttgagtat tccttttctg
     3601 gttaattttg ataaacctga tctctcttta ctgaaagttg ctgattatcc gagtattatc
     3661 ctgttggcca tgacactggg atgtctggaa tatacccttg aagaaggagc gcgctgggga
     3721 tggctggatg ataatactat tttgctgaca tcagttctgg cattggtgag ttttatttta
     3781 tttgctgcgc gtacacttac aatatccaat ccgataatgg atcttcatgc ttttaaggat
     3841 aaa
//
LOCUS       DAZKJR010000255         3823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKJR010000255 DAZKJR010000000
VERSION     DAZKJR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.256"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..854
                     /locus_tag="ACHOWS_005054"
     CDS             <1..854
                     /locus_tag="ACHOWS_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0050486.1"
                     /translation="RAAARKMNISQPALSRTIKELENVLGCNLLIRTRDGIVLTEAGK
                     IFSVHSRMILEEIETAALEVAKVNIDNTGKVAFGISSLFGVTILNNVLDNFRMKYPKK
                     RVMIREGQLSSLIPFLREGVLDFAMGTLTKDMPVGDFIVVELFNAPFTIVARKHHPLR
                     NCTSIDELATARWVFPETQMGYYHYISRLIPFGHPDEPYAPIFTDSVVCIMNLVLYDD
                     YITVLSRARLQEARFKEILCSIPVKYQLPVSQYGLIYPRKRLMTAPAQIMIEYFQQEI
                     QRYDWNG"
     gene            1419..2252
                     /gene="iolR"
                     /locus_tag="ACHOWS_005055"
     CDS             1419..2252
                     /gene="iolR"
                     /locus_tag="ACHOWS_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HFQ0050487.1"
                     /translation="MSKHQTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDT
                     VASIAQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNIN
                     DGEPTPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSF
                     SVACYLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVEL
                     GAKRDAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASMCLAQTLAVSLALAA
                     E"
     gene            complement(2305..3738)
                     /gene="iolT1"
                     /locus_tag="ACHOWS_005056"
     CDS             complement(2305..3738)
                     /gene="iolT1"
                     /locus_tag="ACHOWS_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HFQ0050488.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
BASE COUNT         1137 a          785 c          855 g         1046 t
ORIGIN      
        1 taagagctgc tgcccgtaaa atgaatatat ctcagccagc attaagcaga acgataaaag
       61 aactggagaa tgtattagga tgtaaccttc tgattcgaac aagggatggt atcgttttaa
      121 cagaagctgg aaagatattc tcggtccatt cacgtatgat tcttgaagag attgaaactg
      181 cagctcttga agttgcgaaa gtaaatatag ataacacagg aaaagtagct tttggaattt
      241 cttcgctgtt tggcgtaaca atactgaata atgtacttga taacttccgg atgaaatatc
      301 cgaaaaagcg tgtgatgata agagagggac agttgtcctc ccttatccct ttcctgagag
      361 aaggtgttct ggattttgca atgggtacat taacaaaaga catgccagtt ggagatttta
      421 ttgtggttga attgtttaac gcaccgttca ctatagtggc aagaaaacac catcctttaa
      481 ggaactgtac atcaatcgat gaacttgcaa ctgcaagatg ggtattcccg gaaactcaaa
      541 tgggatacta tcattacata tcccgtctta ttccctttgg acatccggat gaaccatatg
      601 ctccaatatt taccgattcg gtagtttgta ttatgaatct tgttctctat gatgactata
      661 taacggttct ctccagagca aggttacagg aagcaagatt caaagaaata ttgtgttcaa
      721 ttcctgttaa gtatcaacta cccgttagcc agtacgggct tatttatcca cgaaaaaggc
      781 taatgacggc accagcacag ataatgattg aatattttca acaagagata caacgatatg
      841 actggaatgg atgagcaata cccggaatga tggtgtacac cgggataaca atttattcgg
      901 atgtgccaat cccacggttg taatgggcaa tataattgtt agccgattcg ctaatggcag
      961 ggaaaagcgc ggcataaata taagcccaca cacatgtaac gtactgcaga tactcgcttg
     1021 gcagagttgt tacttcttgg gaggcagtac cagccatcag atattcccgt tccatgccaa
     1081 aacctgagtt acccggatac cattacattg cctgtatttc attcatctgc cacacacgtg
     1141 tcagctcttt gtgacctgac gcaatcatct gactcagcag cgattcacgg tactggtctt
     1201 tttggtgacc gttctgacag agcaatacag tgggacaaac gaccatcgtc atcactggag
     1261 aggcgtgcat ggaaactcgt caacaactca agttcgccgg aacggaacgt taacaataga
     1321 actttactgt ataaatttga atactgctaa atgtatgaat ggttcattca tgttaatatt
     1381 gtgatttgca aaaatttcat tttattagag taaaatacat gtctaaacac caaacacagc
     1441 tttccttact gcaggacgac atccgcagtc gctatgacag tctcagcaaa cgtcttaaac
     1501 aagttgctca gtatattctg gataacagta atagcgttgc ttttgatacg gttgcttcta
     1561 ttgcgcagca cgcggaggta cctccttcga cattaattcg ttttgccaat gcgtttggtt
     1621 ttagcggatt caatgaaatg aaacaagtat ttcgccagca tttgatggag gaaaccgtta
     1681 gctatacaga acgggctcgt ctgttcagac aaaaaaacat caatgatggg gaacccacac
     1741 cggaaaaacc gggcgaaatt ctgaagctat tttccatggt aaatgcacag gtactacaac
     1801 aattaccgat gcagattaaa aatgagcaac tggatgctgc agtaagttta ttgtcaaaag
     1861 ccgataatat ttatgtgatt ggattacgtc gttcatttag cgtggcctgt tatttaacct
     1921 atgcattgcg ccatcttgag aggcgtgcat ttttaattga tggtattgga ggcatgtttt
     1981 ctgagcaact gagtatgatc agctctaacg atgtagtcat cgccatcagt tattcgccat
     2041 acgctcagga ggttgttgaa ctggttgagt tgggggcaaa acgtgatgca catcatatcg
     2101 tcattaccga cagtcaggtc agtccgttgg ctgcgttcag tgaggtctgt tttgtaatac
     2161 gtgaagcgca ggtggatggc ttccgttcgc aggtagcgtc gatgtgtctg gcgcagacgt
     2221 tggccgtctc actggctctg gctgctgaat aagaggaaga tgccgataat ctgtttttca
     2281 tgatcatcgg cattttggct aagcttaatc tgaagagcgt tcagcctgaa taggttgtgg
     2341 cttgttcctg cgttttgtca gcacgatatc ctccattttc tctagtgaga cacctttggt
     2401 ttccggcagg tagcggcaaa taaagaaata gctaaagaca cagcaggctg cgaatatcca
     2461 cattggaaat gcaccgtgga aatgggccag taaccaggga ttttcgttaa ttataggaaa
     2521 gaactgtgaa accataaagt ttgctatcca cataaatcct acagaaatac tcattccctg
     2581 tgaacgcatg cggttgggga atatttctga tatgagcacc catgctccaa cgccccagga
     2641 aagtgcatag aaaatcataa agaacagcat accaaagaga gcataatagc cggttgtttg
     2701 gctgtacaga gcccatgaag ttaataataa accaatgatg ctaccaatgg tgccttttcg
     2761 cattagcgat aaacgcccca ttttgtccat gatcattgcg ccaataattg atccgacgag
     2821 ttgaattacg ccaatccaga tggtctgaaa cagcgcttcc tgggcgctac cggtaacatc
     2881 tctcaataca ataggtgcgt aatacatcat aacgttaacg ccggttactt gctgtaacat
     2941 tgcgatcatg caaccgagga aaaggataaa acgtacgtta ctgtcatggt aattgagctt
     3001 ttggtgggcc gtcagccggt cattttgcag agaggttttt atatctgtta gcaaatgtcg
     3061 tgcatgttcc tcgttagata tctttgtaag aatttttagc gtttcttctt cacgaccagc
     3121 catcatcatc cagcgggggg attccggtat aaagaatact aaaatacaga acagaatgca
     3181 tggggcaatg ccggcagcaa acatataacg ccagccaatc tcaatcagcc atgtattggt
     3241 cgcaatcgag gcgattttat agttgacgta aaatatgagg atctgaccaa aaacgatggc
     3301 aaactgttgc atactcaatg ccctgccacg catgtcctta ggtgaaacct ccgacatata
     3361 cattggagat actgttgctg cgaggccgac agcaagtcca cctacgatac gataaataat
     3421 aaagtgcagg aaggtgtagg aaagagatgt tcccactgct gagattgtga acagcagggc
     3481 ggaaatcatg agtgatttct tgcgaccaaa acgtttggat aagtaacctg agctgaatga
     3541 tcccaccaca caacctacaa cgacgctgga aaccgcccag ccggtttcgg ccggagatag
     3601 gtgaaaataa ctggtgagag aactgattgc acctgaaata acggcagtat cgtagccaaa
     3661 taatatcccg cctaatgctg caatagaaca gatacgtaat atatagcctg tattgtaacg
     3721 attcactgat atgggcatag ctgattatct cctgagcaga aatcatctat ctatttgacc
     3781 tataatgaaa aaattgtgtt tggtcatttc cgtagctctg cag
//
LOCUS       DAZKJR010000256         3714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKJR010000256 DAZKJR010000000
VERSION     DAZKJR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.257"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..509
                     /locus_tag="ACHOWS_005057"
                     /pseudo
     CDS             1..509
                     /locus_tag="ACHOWS_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
     gene            750..1715
                     /gene="lipA"
                     /locus_tag="ACHOWS_005058"
     CDS             750..1715
                     /gene="lipA"
                     /locus_tag="ACHOWS_005058"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFQ0050489.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1816..2019)
                     /gene="tatE"
                     /locus_tag="ACHOWS_005059"
     CDS             complement(1816..2019)
                     /gene="tatE"
                     /locus_tag="ACHOWS_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFQ0050490.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2148..2936)
                     /locus_tag="ACHOWS_005060"
     CDS             complement(2148..2936)
                     /locus_tag="ACHOWS_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFQ0050491.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARVLDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFALPQLL"
     gene            3029..3412
                     /gene="crcB"
                     /locus_tag="ACHOWS_005061"
     CDS             3029..3412
                     /gene="crcB"
                     /locus_tag="ACHOWS_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFQ0050492.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3466..3675)
                     /gene="cspE"
                     /locus_tag="ACHOWS_005062"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3466..3675)
                     /gene="cspE"
                     /locus_tag="ACHOWS_005062"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFQ0050493.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT          960 a          972 c          852 g          930 t
ORIGIN      
        1 caaattgaac cctgtttaag cagaaaatcg tcagaaggta aaccacaaat atttacaacc
       61 ttacgaaata ttgatcttac ccttctgact attttgaagt tgcatatgtc cataaaggaa
      121 tcgttaatgc agcgaaagtg cttaatctgc ccccctcggc aatctatcag cctattcaga
      181 aactgcgcgt tatattccct gacctattgt ttattcgcaa aggccagggt gtcactccta
      241 ccgcatttgc gatgcatcta tatgagtcta tcactcaggg acttgagtcc attcttggcg
      301 cgctggatat cgaaggaagc tatgataagc aacggacgat aactattgcg ccctccccct
      361 cggtcggggc ccaggtcctt cctgtcatct atcgggcgat taaaactcac tatccacagc
      421 ttttactgcg caacccgccc atcggcggcg cggaaaacca actctgtcag tttcaaaccg
      481 atctcatcac cgataacatg ttttgcacag gaccatagat gttatccgta atgcattttg
      541 aaaaagtatt gttacagcca gatcacagcg cagaacgata aaatggcaca atccaacaac
      601 aattttacat tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc
      661 ggtcgttaat tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct
      721 tcgtaattcg caactggaac acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg
      781 ttaaataccg cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc
      841 gcgaagccct gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac
      901 gtatccaggg catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag
      961 cctcctgccc taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg
     1021 gcgctatttg tacccgccgt tgtccgttct gtgacgttgc ccacggtcgc ccggtagctc
     1081 cggatgccaa tgaaccagtg aaactggcgc aaaccattgc cgatatggcg ctgcgttatg
     1141 tggttatcac ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccag cactttgcgg
     1201 attgcattac tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg
     1261 atttccgcgg tcgtatggat cgtgctctgg atattctgac cgccacacca ccagatgtgt
     1321 tcaaccataa cctggaaaac gtaccgcgta tttaccgtca ggtacggcct ggtgcagatt
     1381 acaactggtc gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca
     1441 agtctggtct gatggtgggt ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg
     1501 acctgcgccg tcatggtgtg acgatgctaa cgctggggca atatttgcag ccaagccgcc
     1561 atcacctgcc ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag
     1621 cgctggcgat gggctttacc catgctgcat gcggtccgtt tgtccgctcg tcttaccacg
     1681 ccgatttgca ggcgaaaggg atggaagtta agtaatcctg taataattac ttacatctgt
     1741 ctgataaaaa acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc
     1801 gtcctgctcg ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc
     1861 gccttttttc gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc
     1921 tccaaggtct ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc
     1981 cgcaactacc agcagtttgg taatactaat ctcacccata gataccttct tgacatataa
     2041 ccggcaacag aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca
     2101 caatagcaat ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc
     2161 ggcagcgcaa agcgacggtt gtttaagacg ggcagttgcg cgcgtacctg acgcacacgt
     2221 tcgggcgtca cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg
     2281 ccaaagggat ctataatccg gctttgcccg atatttttgt tcccgcactc ccccgccgcc
     2341 accatataac aggtggtatc cagcacacga gcggcaagca acgttgacca gtgatgctct
     2401 ttgagcggcc cacgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaatgcc
     2461 tgtgccagcg ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc
     2521 ccttccacct ccagtaacgg agctatttca ttaccagcat caacatggcg tgattcctga
     2581 atggcaaatg catcatagag atgcagtttg gcataacggg cgacgatgtt tcctgcctgt
     2641 agcgccacca gcatattcca cgcgcgcccc ggtgttgaag gaacatgaat cgtcagaatt
     2701 gtcgtcatca tgttacgttt actttctcgc cgtaaacggt cgaggaattc gccttccagc
     2761 agctgtgctg atttaaccga aagatccgca tcatgatcat cacgcgccag taatgcttcc
     2821 ggcaggacaa acagcgaaac ctcgttttcc gccgcctgcg ccatcaacga ggcacaaatc
     2881 tcagcgttct tttcccacac agatgtaaca gcaaactgtc ctgcggcaac cagcattgcc
     2941 acctcctgtc agagtcgtaa aattcgccaa cggcgattat tcggcgttac acttatcact
     3001 catacaaatc aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg
     3061 cggtggtacg ggaagcgtgg cgagatggct gttaagtatg cgatttaacc cgctgcatca
     3121 ggcgattccg ttggggacgc tggctgcaaa cctgattggg gcattcatca taggaatggg
     3181 attcgcctgg ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac
     3241 cggattttgt ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt
     3301 acaagagggc cgctttggct gggcgttact gaacgttttc gtcaaccttt tagggtcttt
     3361 tgccatgacc gcactggctt tctggctgtt ttccgcctca accgcacact aaagaccaaa
     3421 aaaaacccgc tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac
     3481 gtttgcagca gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc
     3541 agcaagagtt ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct
     3601 gccgtcttcc ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc
     3661 tttaatctta gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgt
//
LOCUS       DAZKJR010000257         3624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKJR010000257 DAZKJR010000000
VERSION     DAZKJR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.258"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(139..250)
                     /locus_tag="ACHOWS_005063"
                     /pseudo
     CDS             complement(139..250)
                     /locus_tag="ACHOWS_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(690..1532)
                     /locus_tag="ACHOWS_005064"
     CDS             complement(690..1532)
                     /locus_tag="ACHOWS_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFQ0050494.1"
                     /translation="MSDITVSRPEVVNWHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLEIMLHLFSGKPIPDNR
                     ENITIHLDDHVQSVQSKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAI"
     gene            complement(1617..1814)
                     /locus_tag="ACHOWS_005065"
     CDS             complement(1617..1814)
                     /locus_tag="ACHOWS_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFQ0050495.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(1826..2065)
                     /locus_tag="ACHOWS_005066"
                     /pseudo
     CDS             complement(1826..2065)
                     /locus_tag="ACHOWS_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            2132..3294
                     /locus_tag="ACHOWS_005067"
     CDS             join(2132..2393,2393..3294)
                     /locus_tag="ACHOWS_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFQ0050496.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          905 a          852 c          887 g          980 t
ORIGIN      
        1 taaacttctt gattacatag tgttgagaaa ggattttact gagaaccaga agtaatattc
       61 cttaccatca aaattcatca tctttgccaa agaaaaatgt tcagaaaata atccatggaa
      121 aattgtccgg agcgcttact attttaatgg attgttagtc tttgcatgag caaacggact
      181 gatacatttc tctttgttct cattcagaaa atctcatcag ttggcgttct gcccgatgtt
      241 gtgctttatg ggtgcgttac gctcctgatg acgtcatttg acgttcaaca gcatcacggg
      301 gccgcacgac atttcacgtc agtcagcgct atagctcaga aacaaatttt cccgaattgg
      361 aatatgcccg caaattgctg gggatgtggg gagaatctgg ttgagttcgg tagaattgat
      421 ttggcggcag gacgcttaaa tcgtggcgtc ctgaaacgaa aaacggacct ccgtggaggt
      481 ccgtttatat gaatttggtg ccggactcgg aatcgaagat ttcatttatc atattgatat
      541 taatctcgtt tatttggttt gatgaaaaat gtaccctctc ttgtaccctc ggtcccttct
      601 ggtggttttt atgagaacaa gtagatcaag atttttgtca tcagaacacg tgtatttttg
      661 tgggttttat tcctgagttt tattcctttt tatatcgcta acgctccggg atagtgctta
      721 gcaataatat cattaagcct gtcaaccagc tctggtttcc tgaacgtgat gtgcgcggag
      781 cccttcttaa agtatctgat gctgaacatc tcatcttcat agtcctcttt actctgaaca
      841 gactggacgt gatcatccag atgaatagtg atgttttccc ggttgtcggg gatcggtttg
      901 ccgctgaaca ggtgtagcat tatttccaga tcagcaagtc gatctgcctg ttgcccggtg
      961 ataagatgaa agccccaccg gtcccacctc accaggttgt tgacgataat tttactgcca
     1021 aatttgcagg ggcaattggt tttgtaattc cagctcaacc ctctgaagac gttgatcacc
     1081 ccccgctcaa acacctcatc cttattctgg tgcagttgtt caaatgtgct cagaatgttc
     1141 gcttcactga tttccggaaa attatcgtat tccagtgacc ggtaccatgt atcacgggcc
     1201 tgtgcatcca ttaaggaaag catcccggaa cgttgcatca ggtcacgcca gatttcgcga
     1261 tcgagattgc gtgtgatggc tttcattgcg gtttcaggtt tctccatcag ccagcaaccg
     1321 cagtgaaaat tctgtttcat ggcccagtcc agtgcggttt taccgccaat gctttctgtc
     1381 attgctgaga tttcagccag ttgccggata agtgtgtcga tttgcagcag tgtactcttt
     1441 cgtacagcca gaatgtggag gatactggtt gagcagataa cgtccgtatg ccagttgaca
     1501 acttccggac gggagactgt gatatcagac ataatcgata ttcctttttg cagataaatg
     1561 aaaacgccag ccctgttcag gctggcgtca gtggaatgaa gtctggtgag tgagagtcac
     1621 cggttctggt gcagaagctg caactggcgc agtgtacgga tatcttccct ggcctgttca
     1681 atgcagggca gcagtgctgc cgaatccgtt ttatcctcat cgttgtcaaa gataattccc
     1741 gattcgcagt cgatattgtc ctgcagccac gcaatcagaa tatccagcgc tgtttccgtg
     1801 gttaatgatt tcatgttgtt tctggttacc agtcgaaagt gggtaaaccc ggcaggcatt
     1861 cagcgctggc atccagatga atgagactga cgccataacg tcgggtaagt gtgacaacca
     1921 gacggcggaa cgtttttgac agccccaggc gtttaagccg cagaaccggg tatgaccacg
     1981 catcggtacg taacagatat ccgctacctg tgaaatgaat ccattctgat tcgccggtgt
     2041 cattcgtctg atgtgacagc gagtactgat cttacccagc aatagtggac acgcggctaa
     2101 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagtaaaaa
     2161 accccgtaaa cagcattcgc ctgaatttcg cagtgaagcc ctgaagcttg ctgaacgcat
     2221 cggtgttact gccgcagccc gtgaactcag cctgtatgaa tcacagctct acaactggcg
     2281 cagtaaacag caaaatcagc agacgtcttc tgaacgtgaa ctggagatgt ctaccgagat
     2341 tgcacgtctc aaacgccagc tggcagaacg ggatgaagag ctggctatcc tccaaaaggc
     2401 cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     2461 gagttcagca tcaaagcaat gtgccgcgtg ctccgggtgg cccgcagcgg ctggtatacg
     2521 tggtgtcagc ggcggacaag gataagcacg cgtcagcagt tccgccaaca ctgcgacagc
     2581 gttgtcctcg cggcttttac ccggtcaaaa cagcgttacg gtgccccacg cctgacggat
     2641 gaactgcgtg ctcagggtta cccctttaac gtaaaaaccg tggcggcaag cctgcgccgt
     2701 cagggactga gggcaaaggc ctcccggaag ttcagcccgg tcagctaccg cgcacacggc
     2761 ctgcctgtgt cagaaaatct gttggagcag gatttttacg ccagtggccc gaaccagaag
     2821 tgggcaggag acatcacgta cttacgtaca gatgaaggct ggctgtatct ggcagtggtc
     2881 attgacctgt ggtcacgtgc cgttattggc tggtcaatgt cgccacgcat gacggcgcaa
     2941 ctggcctgcg atgccctgca gatggcgctg tggcggcgta agaggccccg gaacgttatc
     3001 gttcacacgg accgtggagg ccagtactgt tcagcagatt atcaggcgca actgaagcgg
     3061 cataatctgc gtggaagtat gagcgcaaaa ggttgctgct acgataatgc ctgcgtggaa
     3121 agcttctttc attcgctgaa agtggaatgt atccatggag aacactttat cagccgggaa
     3181 ataatgcggg caacggtgtt taattatatc gaatgtgatt acaatcggtg gcggcggcac
     3241 agttggtgtg gcggcctcag tccggaacaa tttgaaaaca agaacctcgc ttaggcctgt
     3301 gtccatatta cgtgggtagg atcactactc atactataag agggtccact tccccccgga
     3361 tatatgatga tggatggagt atccggagcc cagcatgctc cattagcatc aatatatgtt
     3421 cccatatctt ttccacgtag atctattaca ttagtagaac caggtaatcc attaagattc
     3481 atccctcctt tcacggaatc aagcatgtta tctgatattt ctctcatttt aaaaacctcc
     3541 tgctcattta tgttcagata ttaatttggt gttgcaacca tttattatca cagaattcag
     3601 aatatcattc agttttattt ttgt
//
LOCUS       DAZKJR010000258         3618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKJR010000258 DAZKJR010000000
VERSION     DAZKJR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.259"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..227
                     /locus_tag="ACHOWS_005068"
     CDS             54..227
                     /locus_tag="ACHOWS_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050497.1"
                     /translation="MSYLSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            229..573
                     /locus_tag="ACHOWS_005069"
     CDS             229..573
                     /locus_tag="ACHOWS_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFQ0050498.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGVLLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            577..912
                     /locus_tag="ACHOWS_005070"
     CDS             577..912
                     /locus_tag="ACHOWS_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFQ0050499.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(970..3612)
                     /locus_tag="ACHOWS_005071"
                     /pseudo
     CDS             complement(970..3612)
                     /locus_tag="ACHOWS_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
BASE COUNT          950 a          863 c          784 g         1021 t
ORIGIN      
        1 caacattgta ttaacactca atgacacttc ttgaataaaa atattgtcaa tcaatgagtt
       61 atttatcatt tgagattatt cttattcccg taaaaaacat aattcctatc atcacagtca
      121 cgctcatttt gaattatctg aataatagcg agcgttcttt agttaagcag atcttaattg
      181 aagacgaaat tattgtgtgt gcaacttact taataccgga catctaagtt gaagaaaaaa
      241 atacttgcct tcgggcttat ctcagcattg ttctgctcca cgccagcgat ggcggatatg
      301 aaccgcacca cgaaaggtgt attgctaggc gcaggcgttg gtttacttac tggcaatggt
      361 gttaatggcg tactgaaagg tgcagctgtt ggcgctggtg ttggtgcagt aacagaaaaa
      421 ggccgcgacg gtaaaaatgc gcgtaaaggt gcaaaagtgg gtgccgccgt tggtgctgtc
      481 actggcgttc tgaccggcaa tggcctcgaa ggggcgatta aaggtgccgt gattggcggt
      541 acgggtggtg caattctggg taaaatgaaa taaggaatga tgatgaaaaa atttgcttta
      601 ctggcgggtc tgtttgtttt cgcaccaatg acctgggcgc aggactacaa tattaaaaac
      661 ggtttgccgt cagaaaccta tattacttgc gcagaagcca acgaaatggc aaaaactgac
      721 agcgcacagg ttgcagaaat tgttgcggta atgggtaatg ccagcgttgc cagccgtgat
      781 ttaaaaattg agcaatctcc ggaactaagc gccaaagtgg tagaaaaact gaatcaggtt
      841 tgcgctaaag acccgcaaat gctgttgatc acggcaattg atgacaccat gcgtgctatc
      901 ggcaaaaaat aatatttcat atgttaccgc gtcgatgaat tcgacgcggt tttttagcta
      961 atggcgggat cagaacttat atttcaggcc caccatcatt gcggtgtcat tgtagccatt
     1021 atcccccagc tgcacgccca cattacccca gacgctggcc gccggattca gattgccatt
     1081 tacgccaagt ttaatttccc ccagattacg cgccccgtct tgttcaactt tcacaccgtt
     1141 cattgagaca gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa
     1201 gtcgtcaccg tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaagccgag
     1261 tcgggtctgg atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg
     1321 cgcattatga tcgacgccca tccagatagc ctgtggctgg aagagccacg tatatttcgc
     1381 cgcttccaga ccaaaggatt tattcagggc aaaaccgtaa cccgcttcca gcgagacggt
     1441 tgcacctttc gcgctatagg attcataggc taattcatcc cctttcaccg agtttttaaa
     1501 ccagctatat tacgcccagg tgtcaatgta tgcgcctttt ttactgatgt catcggcaaa
     1561 ccaggtggca tacagccctg cgctatagcc tctgacactc cctttcgaac gataatccga
     1621 cacgctggaa tgagttgagt tgtagtcgcg ggcataacca gccaacactc ccaaacgcca
     1681 actgtcactg tcggtaaaac caccggttaa cagatccgcc cctaactgcg agacgtagcg
     1741 atgcgaggtc gttctcaact gtccgttgct gtcacgccag gcattatgcc cgccaatttg
     1801 acgtagccaa agccggcttg aacgctcctg ttaagttaca ggatcgatgt aacgcgtttc
     1861 acctgcacga tcgttcagac gcataacaaa aagagtgtta gcggctgcca ggttggcgac
     1921 gtaacttcca gcttcagggc gcaggacggg ggtaggcgca acagtaggcg ctgaacttcc
     1981 tccggagttt gtttcaccgc cgctcgttgc ctggaagtta gtcagatacc agtttttatt
     2041 ggtcgcttcg gtattacctc gggttaatga atattcgtag gcaccggcga aaatcctcga
     2101 atccttaata aattctccat ttgattcccc ctcaacgctg ataatctcta tcccatttaa
     2161 cgtgtaagcc ccgctgccgc cagcattggt aactttaact cgggttgttc cagaggtatt
     2221 tccattaata acgactttat ccgtagctga attatcatca cccaattcag ttctgagatg
     2281 caatacgcca ttattaccaa cgtaattttc agttattgtc aatcttgttg gttcgaaatc
     2341 ctttccatct gcccgggaaa tatagaccgt actattatca accgtgagtt tgctcaccgt
     2401 tgagtcagct ttaatatccc aggtactatt atctgaaagc gacagatagg tatgagtgtt
     2461 atcatctgtt gaaagattgg ctgaacccgt tatttttgaa ttagttgcat cgacggtcaa
     2521 atgaccttta atattattaa taaaaacatc tgcagccgat gatacctcac tatccgtaat
     2581 ggtcacgtta ctcgtctcat ttttatggta gaggatttca tttgctgacc gtagtgagct
     2641 accagataaa atatctaccg ttgcttcctg accatttatg gaagactcca gcacaattcc
     2701 agccccctga tgcgcgactg cagatgtatt tttcagcgtc accttgccgc cattataaat
     2761 tttagcaatc gcggcgtcag gacccgttgt ataaatttct ccctggttaa atgaaactgt
     2821 cccctggcga gcagcgatgg catgagcatt gtccccatgc gtggtgatat caacatcggt
     2881 taatgtaacg ctggcattgg tataatcaag cttgctaccc tccttgacga cagtgccatt
     2941 agcgtaagcg gcataacttt ctgttccgtg ggttgttatc tccccttttg ccatatccag
     3001 ttttgctttg gcgttatttt cattacttcc tgtatcggca gtactttctt catcaagaga
     3061 agatacacac gcacgcgcgc ttccgggaga tgcacacgca agaacgccga ctgcgtgatt
     3121 acccgcagta acgataaaag tattacctcc gtcagaaaaa gagggggtag ccgaaccgtc
     3181 agggttgact cttattatgg tatcagtatt caggtttcca tcaacttgac ttaacaaacc
     3241 aacagaataa tctcccgtcg tgacaatcgt attattaccc cgcaaagtga cagcaccgcc
     3301 atattcactt acgactgcgc tagctgtata tgctgtgctt agagaactat tgtcagaagg
     3361 atataaaccg cccgttgtta caatgacgcc attgttaatg gttaaattgc taccctttcc
     3421 tgcaacagat acaccgtaac ttccattacc cgttgttata actagcgcat tatcaatatc
     3481 ttttacttct ccatctttta tttcaacaac atgtttaggt tcatcgtgct gaataaactg
     3541 ttcagcatat gcattaccca ccgccaaagc aataagtcct aaactaaacc cggggagttt
     3601 tttcagtttc atagatta
//
LOCUS       DAZKJR010000259         3617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKJR010000259 DAZKJR010000000
VERSION     DAZKJR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.260"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..305)
                     /locus_tag="ACHOWS_005072"
     CDS             complement(165..305)
                     /locus_tag="ACHOWS_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFQ0050500.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(504..>3617)
                     /locus_tag="ACHOWS_005073"
     CDS             complement(504..>3617)
                     /locus_tag="ACHOWS_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0050501.1"
                     /translation="QSLVETTSKGDRNPSSVRLLIQLERNGHWVTEKDITINGKTTSQ
                     YLMSVILNNLPERPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     MLTHPRYGMGKRLGAPDVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKVW
                     DVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKD
                     RHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLWVI
                     KTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDRE
                     VTLPEAGTSTVNLINGSGKPVRVDITAHPVPDRIQVSALPDGVETYGVWGLSLPSLCR
                     RLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPLSGSLNSVIPPAVQHLTVE
                     VSASDGQYLALAKWDTPRVVKGVRFSLRLTSGNGENSRLVTSAITADTEHRFRGLPPG
                     EYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYFQITVVPHPAVYDPTVQF
                     EFWFSEKRITDTAQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVE
                     ASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETCNEITQ
                     TVNKKLEDQSATIQQIQRVQKETDDSLSALYMLKVQKTKDGIPYVAGIGAGIEDIDGQ
                     PLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFS
                     LTPEGWLTARNADISGHISANSGTLNNVVIAENCTINGTLKAENIIGDLVKCAGVAFP
                     VDGSYLANGTRTLTVYDDHSFDRQIIIPPIIYVGSKQESRTSNDIWTECFLHVDQNGR
                     RIYSGRSVTEPGIFSGIIDMPAGHGHITLSFTVSSRRQNGSFGSSRISNLQAIVVKKN
                     SAGISIR"
BASE COUNT          704 a         1116 c          950 g          847 t
ORIGIN      
        1 cacccgatat tctgactgtc atggatttcc cccataaaaa aaccgcagta ccggtttccc
       61 ggcctgcggt aaaatttgtg gtttgttggt gttaaaacgg tgccatccgg ctgaccaccc
      121 tcagcaaccg gtcggcgggg gatattctcc cccgccacgg tttcttactg cttacactgt
      181 aagaacgccg caatctccgc gcccgctatc cggaaccgga actcgcacag tgaactgtgg
      241 gtgatccaga taatgagcac taccgtgata caaatcacgg tggtttttaa cggtttttgc
      301 gacataaacg cttgctcctt ttacggagag gcgctaacct tcaatttgct gaagatcgaa
      361 agtcaggccc cgggttaaac atgaatgttt gtccggggcc ttcgtctttc cggccttcag
      421 gtgttccctc cggccatcag ccaaaggcac ccgcgcatac tgtacggttt ttgtctcctt
      481 ccggcaatcc cggggcgcga tgttcagcgg atgctgatcc ccgcgctgtt tttcttcacc
      541 actatcgcct gaaggttact gatacgtgaa ctgccaaaac tcccgttctg acgtcgtgaa
      601 cttacggtaa aactcagggt gatatgacca tgaccggctg gcatatcgat gatcccgctg
      661 aaaattcccg gctctgtcac tgacctgccg gaataaatcc ggcgtccgtt ctgatcaaca
      721 tgcaggaagc actctgtcca gatgtcatta ctggtgcggg attcctgttt tgacccgaca
      781 tagattatcg gcgggattat aatctgccgg tcaaagctgt gatcgtcata caccgtcagc
      841 gttcgtgtac cgttcgcaag gtaactacca tccaccggaa aagccacccc tgcacatttc
      901 acaagatcac caataatgtt ctccgctttc agcgtgccat ttatcgtaca gttctccgct
      961 atcacgacat tattgagcgt gcccgagttc gcactgatat gtccgctgat gtccgcattg
     1021 cgggccgtca gccagccctc cggcgtcagg gagaacgtcg ggggattgcc ggacgaggtg
     1081 atgctcactg caaacagtcg cttcaggaac acatcgttca tgaacagctg attcccctgc
     1141 gccacaaaca gcggcgtggt gttgccgttc tccgggttaa tcatcgcgat acggtccgcc
     1201 tgcagcagaa tattgctcag gggctggcca tctatatcct caatccccgc accaataccg
     1261 gcaacatacg gaatgccgtc ctttgttttc tgcaccttca gcatgtacag tgcgctcagg
     1321 ctgtcatccg tctccttctg cacacgctgt atctgctgta tggtggcact ctggtcttcc
     1381 agctttttat tgaccgtctg cgtgatttca ttacaggttt ctgtgatggt ggtcttcatc
     1441 tccgccatct catcgtcaag ctgactgtta tcaatcagtt cccacatccc ctgtgccaga
     1501 tgtgtcttcc cgatttctcc tctgaaaaag tccagatacc cttccgcatc attgctcgcc
     1561 cggccactgg cttccacaaa cgcagatttc cccaccagat tgacgctgcg cacataaaac
     1621 cagaaatcct tccccggctt aatgtgcggg ccggagacac tccactggct gcctgtcccc
     1681 agataacggg cagaggtttc cacctgtgcc gtgtccgtga tgcgtttttc tgagaaccag
     1741 aactcaaact gtaccgtcgg gtcatacacc gcaggatgcg ggaccaccgt tatctgaaaa
     1801 tagccgggcg tcagttcaat gctggccggt gctgccgggg cgttaatccg gaacgtggtg
     1861 gtggcaggtt cgccctgctg gccgtagctg tttatcgccc tgaccgtcag ggtgtattcc
     1921 ccgggcggca ggcctctgaa acggtgctcc gtgtcggcag tgatggcgct ggtcaccagg
     1981 cggctgtttt caccgtttcc gctggtcagg cgcagactga agcgcacgcc cttcaccacc
     2041 cgcggcgtgt cccatttcgc cagcgccaga tactggccgt ctgaggcact cacctccacc
     2101 gtgaggtgct gcactgccgg cgggatgacg ctgttcagtg agccggagag cggctcaaag
     2161 cgggccccgt tatccacaat ggcttctttt tccggcacat gctgcaccgc cgtgatggca
     2221 aaggtgccgt ccgtgttttc ccggatggaa acacagcgga acaggcgacg acacagtgac
     2281 ggcagggaga gtccccacac accgtatgtc tccacgccat ccggcagggc gctgacctgt
     2341 atccggtcag ggacggggtg tgcagtgatg tccacgcgca ccggcttacc gctgccgtta
     2401 atcaggttca ccgtcgatgt acctgcttcc ggcagtgtca cctcacggtc cagcgtcagt
     2461 gtgcggctgg cggcatcgat ggacaggata cgtccgccgg tcagggtccc ggcatagtcg
     2521 ttatcacaga tttcaatgat gtcaccgggc gtgtgccgca gcccctgtga cccgagcgtg
     2581 aaatccaccg tctgcgtttc cagcagttcg gtctttatca cccacagtcc ggcacggtga
     2641 gcctgaccgc ggctggtaca gccgaacgca tccatcttca gcaggttgcg cccgtagcgc
     2701 aggatggcgt ccgggtcttc caccagttcc gtggaagtct gccagccgtt ctgcgggtca
     2761 gtgtaattca cctccaccgc cgtgtgccgg tccttcaggg cactgaagct gtagcggaac
     2821 cccacgccgt tatcatccac caccacatcg ctgttggtgt acggccacac cacatccgac
     2881 ggacggtcct gaacgaacgt cagcgtctga ccgttccata ccggcataca gcgcatcgcc
     2941 gagcagaaat cccccaggac atcccagacc ttacgctgct gtgacaggta cgcattgaaa
     3001 gtcatccgcg gctctgttcc cccgaaacca tccgggaccg tctggtcaca gtactgcccg
     3061 atggcataca gtgcccactt gtccacgtcc ggggccccca ggcgttttcc catcccgtag
     3121 cgcgggtggg tcagcatgtc ccacaggcac caggccgggt tattgctgta tgccggtttc
     3181 agactcccgt cccagatacc gctgtaggtg cgtttttccg ggtcatagtt tgacggcacc
     3241 tgaatgatgc gaccacggat atggtagttc accgtcagct gctggccacc aaactgctcc
     3301 gcatccacct gcagccccac aatcgccgtg ttcgggtagc actgtttcac atcgatgatt
     3361 tcggtgtatg acgaccacag cgttctgttc tgcagctggt ccgtggtgct gtccgccgtc
     3421 tccctgacca tccggatgtt aaaggggcgc tcagggagat tattcagaat caccgacatc
     3481 aggtactgcg aggtggtctt gccgttaatg gtgatatcct tctccgtcac ccagtgcccg
     3541 ttacgctcaa gctgaatcag cagccggaca gaagagggat tacggtcacc ctttgaggtg
     3601 gtctccacca gtgactg
//
LOCUS       DAZKJR010000260         3604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKJR010000260 DAZKJR010000000
VERSION     DAZKJR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.261"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..665
                     /gene="ybcL"
                     /locus_tag="ACHOWS_005074"
     CDS             114..665
                     /gene="ybcL"
                     /locus_tag="ACHOWS_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFQ0050502.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            675..1472
                     /locus_tag="ACHOWS_005075"
     CDS             675..1472
                     /locus_tag="ACHOWS_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0050503.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGYEFYIENYPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIR
                     NKTSFFLPQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSK
                     QSLISRCYNLLLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            1589..1690
                     /locus_tag="ACHOWS_005076"
     CDS             1589..1690
                     /locus_tag="ACHOWS_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050504.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            1687..2142
                     /gene="ybcN"
                     /locus_tag="ACHOWS_005077"
     CDS             1687..2142
                     /gene="ybcN"
                     /locus_tag="ACHOWS_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFQ0050505.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            2142..2312
                     /locus_tag="ACHOWS_005078"
     CDS             2142..2312
                     /locus_tag="ACHOWS_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFQ0050506.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            2305..2595
                     /locus_tag="ACHOWS_005079"
     CDS             2305..2595
                     /locus_tag="ACHOWS_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFQ0050507.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            2592..2954
                     /gene="rusA"
                     /locus_tag="ACHOWS_005080"
     CDS             2592..2954
                     /gene="rusA"
                     /locus_tag="ACHOWS_005080"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFQ0050508.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            2951..3091
                     /locus_tag="ACHOWS_005081"
     CDS             2951..3091
                     /locus_tag="ACHOWS_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFQ0050509.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            3177..3560
                     /gene="quuD"
                     /locus_tag="ACHOWS_005082"
     CDS             3177..3560
                     /gene="quuD"
                     /locus_tag="ACHOWS_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFQ0050510.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT         1031 a          731 c          855 g          987 t
ORIGIN      
        1 attgggttgg gggtacaatt gtgattgctt ttgcatgaac attgcgcctt tatgcataat
       61 gagataaagg aatatcaaat aaaataacga taggtcataa caaagaggtt tttatgaaaa
      121 cacttatcgt ttcaactgta ttggcattca taacattttc tgcgcaggct gcagcatttc
      181 aggtcactag taatgaaata aaaacaggag agcaacttac aacgtctcat gtcttttctg
      241 gatttgggtg tgaaggtggt aatacatcgc cctcattaac ctggtctggt gttcctgaag
      301 gtaccaaaag ctttgccgta actgtatatg atccagatgc acctacaggc agtggttggt
      361 ggcattggac tgttgttaat attccagcaa cagtaacata tttgcccgtt gatgcaggga
      421 gacgtgatgg aacaaaactg ccgactggtg ctgttcaagg ccgaaatgat tttggctatg
      481 ctgggtttgg tggcgcatgt cctcctaaag gagataaacc acatcattac cagtttaaag
      541 tatgggctct aaaaactgaa aagattcctg tagattctaa ctccagcgga gcgttagttg
      601 gttatatgct taatgctaat aaaatcgcaa ccgctgagat aacaccagtt tatgagataa
      661 agtagggtga gagtatgctg gcaagaggta agactaactt aaagatcgaa gaaatacgga
      721 tgcataaaca tcatgagatt catagggtta agcctcttat gccagctttg tgtcgtatcc
      781 gtcagggaaa gaaagttatc aattgggaga cgcatacttt aactgttgat aataatcaaa
      841 taatattatt tccttgtggt tatgaatttt atattgagaa ttatcctgaa gcagggcttt
      901 atcttgcaga aatgctttac ttacccattg atttaattga gagtttccaa aaactttata
      961 cggtaactga tcaaatacgt aacaaaacaa gtttcttttt acctcagaat cctgagttaa
     1021 tatattgttg ggagcaacta aaaacatctg tttcccgagg cttctcaact aaaattcagg
     1081 agcacttagc aatgggcgtt ctactttcgt taggagtgaa tcatgttaat catttacttt
     1141 tatcatatag taaacaatca ttgataagtc gttgttataa cctgctgcta tccgaacccg
     1201 gcacaaaatg gacagcaaac aaggttgctc gatatctcta catttctgtt tctacattac
     1261 atcgccgtct agcaagcgag ggggtaagtt tccaaagtat actggacgat gtgaggttaa
     1321 ataatgcgtt gtctgctata caaacgacgg taaaacctat aagcgagatt gccagagaaa
     1381 atggttataa gtgtccttct cgttttactg aaagatttca taatcgtttt aatataacac
     1441 caagagagat aagaaaagct tccagagagt aaaagtgttt taagaaggag caattctatc
     1501 gattttgatt ttgggaaatc aacacggcat aattatgtca ccggagcctg aacaactccg
     1561 gtgacttctg cgctaaacgg ggacgtttat gcgcacatac aatccaaact ctcttctccc
     1621 ttcacagatg cagaaatgca cctgcaattc tttgcatcta gcgtttgacc tctgcggagg
     1681 tgaagcgtga acctctcaca agacggcatc aaattacatc gcggcaactt caccgctatc
     1741 ggtcggcaga tccagcctta tctggaggag ggcaaatgct ttcgcatggt gcttaaaccg
     1801 tggcgtgaga aacgcagtct ttcccagaat gcactcagcc acatgtggta cagcgaaatc
     1861 agtgaatacc tcatcagcag gggtaaaacg ttcgccactc cagcttgggt aaaagatgct
     1921 ctcaaacaca catatctcgg ttatgaaacc aaagacctgg ttgatgtcgt aaccggtgat
     1981 atcaccacta tccagtcgtt acgccatacc tccgatcttg ataccggaga gatgtatgtc
     2041 ttcctgtgta aggttgaagc ctgggcggtg aatattggct gccacctgac tattccgcag
     2101 agctgcgagt tccagctgct ccgcgacaag caggaggcgt aatggctaca ccgcttattc
     2161 gtgtcatgaa cggacacatc tacagagtat caaatcgtcg taagcgtaag cctgagctga
     2221 agccatccga aataccaaca ctgctcggat ataccgctag cctggttgat aaaaaatggt
     2281 tgcgactggc agcaaggagg aatcatggct gatttgagaa aagcagcgcg tggtcgggaa
     2341 tgccaggtaa gaatccctgg cgtatgtaat ggcaattctg aaacgtctgt actggcacat
     2401 atccggctgg ctggattgtg cggtaccggt atcaaaccgc cagacctgat tgccaccatt
     2461 gcatgttctg cctgccacga cgaaatcgac cgccgcacac attttgtcga tgctgcatat
     2521 gcaaaagaat gcgcgctgga aggtatggcg agaacacagg ttatctggct gaaagagggg
     2581 gttattaagg cgtgaatacc tacagtatca cattaccctg gcctccgagc aataatcgct
     2641 attaccgcca taatcgcggg cgcacgcacg tcagtgcaga ggggcaggca taccgcgata
     2701 acgtcgcccg aatcattaaa aacgcaatgc tggatatcgg cctggctatg cctgtgaaaa
     2761 tccgcattga gtgccacatg ccggatcgcc gtcgccgtga cctggataat ctgcaaaaag
     2821 ccgcttttga cgcactcacc aaagcaggtt tctggctgga tgatgctcag gtcgttgatt
     2881 accgcgttgt gaagatgcct gttaccaaag gtggcaggct ggaactgacc atcaccgaaa
     2941 tggggaatga atgatgtttg agttttatat ggcagaactt cttcgccacc gctgggggca
     3001 tctgcgctta tatcgtttcc ccggttctgt tttgaccgat taccgaatac tgaagaatta
     3061 cgccaaaacc ctgacaggag caggagtatg aagtcagaga taacaatcaa ctaatactgt
     3121 tttgttgatt tttgcttgta attggcgttc tggtctgatt tttgtggagt aagttgatgc
     3181 gtgatattca gatggttctt gagcgttggg gagcgtgggc ggctaataat catgaagatg
     3241 tgacctggtc gtccattgcc gccggtttta agggattaat tacttcaaaa gtaaaatctc
     3301 gcccgcaatg ttgtgacgat gacgcgatga ttatttgcgg gtgcatggcc cgtctgaaaa
     3361 agaacaacag cgatttgcac gatttattag tagattatta tgtagtcggt atgacattca
     3421 tgtcactggc aggtaagcat tgctgctctg atggttatat cgggaaaagg ttacagaagg
     3481 ctgagggcat aattgaaggg atgttaatgg cattagatat ccggttagag atggatatcg
     3541 ttgttaataa ctctaattaa tatgccaatt gtttactaaa aattattaaa aatggggcgt
     3601 tgca
//
LOCUS       DAZKJR010000261         3578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKJR010000261 DAZKJR010000000
VERSION     DAZKJR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.262"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(352..441)
                     /locus_tag="ACHOWS_005083"
     tRNA            complement(352..441)
                     /locus_tag="ACHOWS_005083"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(405..407),aa:Ser,seq:cga)
     gene            535..1332
                     /gene="mtfA"
                     /locus_tag="ACHOWS_005084"
     CDS             535..1332
                     /gene="mtfA"
                     /locus_tag="ACHOWS_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFQ0050511.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1433..1508
                     /locus_tag="ACHOWS_005085"
     tRNA            1433..1508
                     /locus_tag="ACHOWS_005085"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1466..1468,aa:Asn,seq:gtt)
     gene            1821..2594
                     /locus_tag="ACHOWS_005086"
                     /pseudo
     CDS             1821..2594
                     /locus_tag="ACHOWS_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            2644..>3578
                     /locus_tag="ACHOWS_005087"
     CDS             2644..>3578
                     /locus_tag="ACHOWS_005087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HFQ0050512.1"
                     /translation="MAGPADASQSVLKNNRSSLKGDFTESAELYLVLHDLSGHPINVS
                     EGLEFVQSGTNVSYVQISTIDYTQNLYGEYKATVTGGGEGIATLIPVLNGVHQAGLST
                     TIEFISAGTRPMNGTVSVNGANLPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSS
                     TASWVDVDTSGKVTFKNVGDRNAVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLS
                     QAENYCSNQVGNGYPLPRADLLSNGHMRREIGSLYGEWGDMGNYMKEADFYSMVYWSS
                     NSAGAGQQYIVSLETGTQNTYQTYEFFYGACYKQI"
BASE COUNT          947 a          812 c          854 g          965 t
ORIGIN      
        1 caacaactct tggaaattat ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct
       61 tttttggctc aattattgac cccttctcta cgcaacttca gttcccgcca gcaactttgc
      121 ggcagtttca taggatcaat gtctaaaaga ataatggtga tcgataagaa aacgactgaa
      181 taactgtaga ttttcgctcg aaaccttcct gtcagctcca tagcgaatca agtgctgaat
      241 gtcacagtat cgaacagaaa acagtgacga tctaaccctt caagaatatt ctacgattgt
      301 tctgtttagg aaaagcaagg cgggaagtcg ggagataagt cattgataaa gtggcggaga
      361 gagggggatt tgaacccccg gtagagttgc ccctactccg gttttcgaga ccggtccgtt
      421 cagccgctcc ggcatctctc cgttcagatg gttgccatga tgccaggaaa tttggcattt
      481 taacagtccc tgtccgtgca attttgttca agtgacgagt ttgcgagcaa aacgatgatt
      541 aagtggccct ggaaagtaca agaatcagca catcaaactg cccttccctg gcaggaagca
      601 ctatcgatcc cccttttaac gtgtctgacg gaacaggaac aaagcaaatt agtcactctt
      661 gccgaacgtt ttttacagca aaagcggctt gttcctttac agggctttga actggattca
      721 ttaagaagct gccggatagc acttctattt tgcctgcccg ttctggagtt aggactggaa
      781 tggctggatg gttttcatga agtcttaatt tatcctgcgc catttgtggt cgatgatgaa
      841 tgggaagacg atatcggtct ggtgcataac caacgtattg ttcagtcagg tcagagctgg
      901 cagcaagggc ctatcgtttt gaactggttg gatatacaag attcttttga tgcttctggt
      961 tttaacctga ttattcatga agtcgctcat aagctggaca cccgtaacgg cgatcgcgcc
     1021 agcggagttc cctttatttc gttgcgtgag gttgctggct gggaacacga tcttcatgct
     1081 gcaatgaaca acattcagga agaaatcgaa ttagttggtg agaatgcggc gagcattgat
     1141 gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac tttctgaata tttctttagc
     1201 gccccagaac tttttgctcc tcgtttccct tcattgtggc aacgtttctg tcaattttat
     1261 caacaagatc ctttgcagag actgcatcac gctaatgata cagactcgtt ttcggcgacg
     1321 aatgttcatt aattaacaac tttgcagatt aattaaccaa ttgaaatggc ttatgaaatt
     1381 tagtgttgac agacaaggta ccgctaagta atatgcgccc cgttctcacg attcctctgt
     1441 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
     1501 aggagccaaa ttctaaaaat tcgcttttta gcgcaatgtc actgacctta gttgagcatt
     1561 gctttttaac ggatagcggg tttttgacat cttaagcgcc ctcgaccttt atggttgagg
     1621 gcgttttgct atgaacgcca tcaccatttt cccctcgatt acaaaacttg agtaattcag
     1681 tagtctcccc tcttgcaact cacacccaaa actgcttaac gaaaagttat taattttcaa
     1741 tcatattgct atcaggattt acattttttc gctgtgctag aaagggcgca tttatgttag
     1801 ctcgttcagg gaaggtaagc atggctacga agaagagaag tggagaagaa ataaatgacc
     1861 aacaaatctt atgcgggatg ggtattaaac tacgccgctt aactgcgggt atctgcctgg
     1921 taactcaact tgtgttccct atggctgcgg cagcacaagg tgtggtaaac gccgcaaccc
     1981 aacaaccagt tcctgcacaa atcgccattg caaatgccaa tacggtgccc tatacccttg
     2041 gagcgctgga atcggcccaa agcgttgccg aacgtttcgg tatttcgttg gctgagttac
     2101 gcaaactcaa ccagtttcgt acgtttgctc gaggttttga taatgtccgc cagggtgatg
     2161 aactggatgt cccggcacaa gttagtgaaa aaaaattaac cccgccgccg ggtaatagca
     2221 gtgacaacct tgagcaacag atagccagta cttcacagca aatcgggtct ctgctcgccg
     2281 aagatatgaa cagcgagcaa gcggccaata tggcgcgtgg atgggcctct tctcaggcat
     2341 ctggcgcatt gacagactgg ttaagccgct tcggtaccgc aagaatcacg ctgggcgtgg
     2401 atgaagattt tagcctgaag aattcccagt tcgattttct ccatccatgg tatgaaacgc
     2461 ctgataatct ctttttcagc cagcataccc tccaccgtac tgacgaacgt acgcagatta
     2521 acaacggctt gggttggcgt catttcactc ccacatggat gtcgggcatc aacttctttt
     2581 tcgaccacga tcttaagcgc agaaacgagc tatggtgggg cgcaaacagt agatataacg
     2641 ctggtggcag gcccggcaga cgcctcgcag tccgtcctta aaaacaaccg gtcatcactg
     2701 aaaggggact ttaccgaaag tgctgaacta tatcttgttt tacacgatct atcaggtcat
     2761 ccgatcaatg tttctgaagg gctggaattt gtgcaatcag gtaccaacgt gtcctatgtg
     2821 caaatcagca caattgatta cactcaaaat ttatacggtg agtataaagc cactgttaca
     2881 ggcggcggag agggtatcgc aacgctgatc cccgtgttga atggtgttca ccaggcgggt
     2941 ctgagtacca cgatagaatt cattagcgcg ggaaccagac caatgaacgg tacagtatca
     3001 gtgaatggtg caaacctacc tgcagcttca ttcccttcgc agggatttac cggggcgtat
     3061 tatcagttga ataatgacaa ctttgcacca ggaaaaacgg cggctgatta tgcgttttca
     3121 agcactgcct cctgggttga tgttgatact tccggtaagg tgacattcaa aaatgttgga
     3181 gatagaaatg ctgtaataat cacggcgacc ccaagatcag gtggcgctat ttaccaaacg
     3241 caagtacggg tgaagggctg gtgggtgaat cacggtaata acctcatgca attaagccag
     3301 gctgaaaact actgcagcaa ccaggtcggt aatggctacc cccttccccg cgcggacctt
     3361 ttaagcaatg ggcacatgcg gcgcgaaata gggtcactgt atggtgagtg gggagatatg
     3421 ggaaattaca tgaaggaagc tgatttttat tcaatggtat attggtcttc taattccgca
     3481 ggcgcaggcc aacaatacat tgtatccctg gaaacaggga cgcaaaacac ttatcaaacg
     3541 tatgaatttt tttatggggc gtgttataaa cagattta
//
LOCUS       DAZKJR010000262         3453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKJR010000262 DAZKJR010000000
VERSION     DAZKJR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.263"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..868
                     /locus_tag="ACHOWS_005088"
     CDS             2..868
                     /locus_tag="ACHOWS_005088"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFQ0050513.1"
                     /translation="MAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGALRL
                     LVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSVIND
                     AQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSGVTD
                     TECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSASTI
                     AELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSDSGL
                     IGATLWDRDCIT"
     gene            917..2389
                     /locus_tag="ACHOWS_005089"
     CDS             917..2389
                     /locus_tag="ACHOWS_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0050514.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            2393..3223
                     /locus_tag="ACHOWS_005090"
     CDS             2393..3223
                     /locus_tag="ACHOWS_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0050515.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            3269..>3453
                     /locus_tag="ACHOWS_005091"
     CDS             3269..>3453
                     /locus_tag="ACHOWS_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HFQ0050516.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNT"
BASE COUNT          912 a          552 c          865 g         1124 t
ORIGIN      
        1 actggctgtt gatataggag gaacgaaaat ttcagcggcg ttgatatctg atgacggttc
       61 atttttgctg aaaaaacaga tatcaacacc tcatgaaaga tgtcctgacg aaatgacagg
      121 ggctttacgt ctactggttt ctgaaatgaa gggaacagcg gagcgatttg cagtagcatc
      181 aacagggatt attaataatg gagtattgac agctttgaat ccagacaatc ttggagggct
      241 gaaagagtat cctctgaaaa atataatgga agatataact ggtttaaatg gaagtgtgat
      301 aaatgatgcc caggcagccg catgggcaga atatacagtt ttgccaaaag aaatttgtga
      361 tatggttttt ataaccgttt ctacgggggt tggaggggga attgtagtta acagaaaatt
      421 attaacaggt gtttctggat tagccggaca tgttgggcat atattgtccg gagtaacgga
      481 tacagaatgt ggatgtggtc gtcggggatg cgttgaagcg gtttcatctg gtcgggccat
      541 catgggggca gcaaaaaatg aacttgcagg atatagcacc aaatatattt ttgaattagc
      601 gagacaggga tataaggaag ccgaattttt gacagagcgt tctgccagta ctattgctga
      661 attaatagta agtttaaaat tactgcttga ttgtcaggtt gttgttgtcg gaggaagtgt
      721 tggacttgct gatgggtata ttcagaaagt gtcgaaacat ctttccatat attctgaaat
      781 ttgtaatgta atgctgtttc cggcatattt cagaagtgac tctggtctta ttggtgcaac
      841 tctttgggac cgggattgta ttacttaaag taatactcca gtgtattata aatccgctat
      901 tattgtgagg tttaatatgg actcctgtat tcagaaaaag caggtttggt ataagcatct
      961 ttcacccaga cagtggaaga tattcggtgc ggcatggaca ggttatttac ttgatggttt
     1021 tgatttcgtt ctgatatctc tggttcttac agaggttcag catgaattcg gcttgacgac
     1081 aatagaagcg gcaagcctta tttccgctgc ttttatttcc cgatggtttg gggggcttgc
     1141 tattggcgct ttatcggaca agatggggcg caggatggca atggttttaa gtatagtgct
     1201 attctctctc ggaacccttg cctgtggtct ggctcctgga tatgcagtga tgtttattgc
     1261 tcgcattgtt attgggcttg gtatggcagg tgaatatggc tcaagtgtaa catatgttat
     1321 cgaaagctgg cctgttcatc tcagaaacaa ggcaagtggt tttttaattt ctggattttc
     1381 tattggggga ggtttagctg cacaggttta tagcattgta gtaccactat ggggctggcg
     1441 ttctcttttc tttgtcggca tgcttccaat attatttgct ttttatcttc gcaagaatct
     1501 tccggagtct gatgactggc aaaaaaggca acaagagaat aaacccgtca gaacaatggt
     1561 ggatattctg tacagagaaa agaataaata cataaatatt cttctgtctt gtattgcatt
     1621 tgcatgtcta tatgtttgtt tttcaggcgt cactgcaaac gctgctttaa tcacagttat
     1681 ggcattgtgc tgtgcggctg tttttatcag ttttatatat cagggtatgg ggaaaagatg
     1741 gccaacaggc attatgttga tgcttgtggt tatgttctgt ttcctctacg gatggccact
     1801 gcaggctttt cttcctacat ggcttaaggt tgatatgcag tattcaccag aaactgttgc
     1861 tctcatattt atgctggcag gttttggctc tgcagctgga agttgtatag gtggttttat
     1921 gggggactgg cttggaaccc gaaaagcata tgttatatca cttcttatcg gtcagcttgt
     1981 tattatcccc gtttttcttg tggaccggga ctatgtatgg ttacttggcc ttctgatctt
     2041 tactcagcag gtttttggtc aggggattgg tgctctggtg ccgaaaataa tcagtggtta
     2101 ttttaatgtg gagcagcgtg cggcaggact ggggtttatt tataatgtag gttcacttgg
     2161 tggagcatgt gcgcctattc ttggtgcagt tgttgcgtct catacaagtc ttggtacagc
     2221 aatgtgttct cttgctttta tcttaacatt tgttgttctt gtccttattg gttttgatat
     2281 gccatcacgt gtacagagat ggatccatcc agaagctgcg cttgaatatg atactgtcga
     2341 tggaaaacct ttttatggag caagaaaaaa gaatgttgca ggagaataaa ggatgctatc
     2401 agggaataaa aaaatgattc attacaggga gaatatatat tctcttgata attatgagcg
     2461 ttacataact gacaattcgc tatttgagaa tactgacgat ggcataatat atacaggtat
     2521 tgtgcccctt attacagttg ttgagcctgt tgtatctaat caacatagtg tgctgattat
     2581 acctggaggt ggatacagga ggattgttta tgaaagagag ggacttgatg taggcagatg
     2641 gatagcaggt cagggatata cggcatttat tctttcttac agaatgccct ccgttaatgg
     2701 ttattattcc tttgcggatg cgaagaatgc cctgaacttt atttcttact gggccggaat
     2761 gtcggagaga acatttaaga gtgttggatg tatggggttt tcggcaggtg gtcatcttgc
     2821 tggatatatt actcatctcc attatgagga agagacttct gtgcgtcctg actggatggc
     2881 tctgatatat ccagttatat ctcttgacag gagtattgct catcagggat gccggaaacg
     2941 tcttctggat gcaggtgaaa gtgaatattc agccagtctt gaaaatagtg ttacagagta
     3001 tatgccacca gtctttattg tgcatgcaac agatgataag gatgttccag ttattcactc
     3061 tctgagatat tttaactcta tccgggataa gggacatcat gcagaagctc atttttttga
     3121 taagggaggg catggatttg gtgtatccgg aaaaaatgga tatccgatag aaaaatggca
     3181 aagcctgctt ctggaatggc ttgcggaaca taagtttcat tgagaaatca gattacaagt
     3241 gataaatata tttaatgatg aggcatttat gaaaaaatat tatccactat taactgtgat
     3301 tcttcttggt ttacctttgt gctctcaggc attaactctt aatctacgtg gaggatacag
     3361 aagcgccagt cacgcttatg aaacgttgat tcaggttagt caggggtgga ataatggttg
     3421 gtgggcaagt tttgagtcaa atacctggaa tac
//
LOCUS       DAZKJR010000263         3423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKJR010000263 DAZKJR010000000
VERSION     DAZKJR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.264"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..621)
                     /gene="dsbA"
                     /locus_tag="ACHOWS_005092"
     CDS             complement(<1..621)
                     /gene="dsbA"
                     /locus_tag="ACHOWS_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFQ0050517.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEK"
     gene            complement(638..1624)
                     /gene="srkA"
                     /locus_tag="ACHOWS_005093"
     CDS             complement(638..1624)
                     /gene="srkA"
                     /locus_tag="ACHOWS_005093"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFQ0050518.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTPDQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1701..1970)
                     /locus_tag="ACHOWS_005094"
     CDS             complement(1701..1970)
                     /locus_tag="ACHOWS_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFQ0050519.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2040..2624
                     /gene="mobA"
                     /locus_tag="ACHOWS_005095"
     CDS             2040..2624
                     /gene="mobA"
                     /locus_tag="ACHOWS_005095"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFQ0050520.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWIHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            2606..3133
                     /gene="mobB"
                     /locus_tag="ACHOWS_005096"
     CDS             2606..3133
                     /gene="mobB"
                     /locus_tag="ACHOWS_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFQ0050521.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALMDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(3236..3351)
                     /gene="rrf"
                     /locus_tag="ACHOWS_005097"
     rRNA            complement(3236..3351)
                     /gene="rrf"
                     /locus_tag="ACHOWS_005097"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          888 a          841 c          827 g          867 t
ORIGIN      
        1 tttctcggac agatatttca cagtatcagc atactgctga acaaaaacat ccatattgct
       61 ggtatccata ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacgcc
      121 acgcaattgc acgtcagctg cagctttttc ctgctgagcg accagggatt tcaccacgaa
      181 gctgttccac gctgcgtcgt actcttcacc tttaatacct gcgttgataa atacatcgcg
      241 gatatcagat gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt
      301 gactttgtct tccacgccca gtgccatcgc cactgcccat gcctgagtca gatctttgcc
      361 caggtcaccc cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt
      421 tttcttcacg ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa
      481 gaaagagaaa aactccagca cttgcggcgc gccagcaact ggtttttcca gggtagtgta
      541 ctgtttacca tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag
      601 cgccagccaa atctttttca tgatctactc tctccgatta atacataggt gttaattgca
      661 aagggggttc ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgcagccagt
      721 aatcttcccc ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caacgccgca
      781 ttagccaggc aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt
      841 cagcggtgtc gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc
      901 gctgctcggc tttatcgcca ttgagcaaca tccacaaatc ctgaatagcc ggaccattac
      961 gtgcatcatc cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc
     1021 agtctccatg tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga
     1081 tcagctcatc tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt
     1141 cctcaaacag cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgga
     1201 taaaaagctg tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca
     1261 tctgatcgat attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat
     1321 aaaatccctg atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa
     1381 cttcatcatt taccaactgc aacgcaaatt gatgttcttc gaggatttga tcgggtgtcc
     1441 aacgttcagg acgataaaat ttgacgacaa aacgtcgacg atcttcgtcc tgaaattgat
     1501 agacacggtt ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct
     1561 caaacagagc atccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt
     1621 tcatcccatc atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg
     1681 gtggcaccgc ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt
     1741 ttgaagtctt cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca
     1801 cgcattttca ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa
     1861 cctgactctt tcgccagttt ctgcaaaaat tgcagcaggt taaggtctgg ctctttttgc
     1921 caggctggct ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc
     1981 cttactcttg agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg
     2041 tgaatctgat gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg
     2101 gcggcgtaga taaaggattg cttgaattaa acggcaaacc attatggcaa catgtcgctg
     2161 acgcgcttat gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct
     2221 atcaggcaag cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg
     2281 caggaatgct ttcagtaatg cagcaggaag cgggtgagtg gtttttgttt tgtccgtgcg
     2341 atacgcctta cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc
     2401 ctgtcgtgtg gatccacgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg
     2461 caattgagcc tttattactg gaatatctgc aagcaggaga acgccgggtt atggcattta
     2521 tgcgtctggc tggcggtcat gcggttgatt tcagcgatcg taaagaggca tttataaacg
     2581 tgaatacgcc agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt
     2641 tgccgcgtgg agtggtactg gaaaaactac actgctgaaa aaactgatcc cggtattatg
     2701 cgccagaggg atccgtccgg gactgattaa gcatacgcac catgatatgg atgttgataa
     2761 accgggcaaa gatagctatg agttgcgcaa ggctggcgcg gcacaaacca tcgttgccag
     2821 ccagcaacga tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt
     2881 tctcgcaagt cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca
     2941 tgaagagatc gcaaagattg tgctgtttcg cgatggagcc ggacatcgac cggaagaatt
     3001 agtgatagac agacatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc
     3061 gttaatggat attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa
     3121 gcaggacgga taagcaaaag taggctggat aagacgcgcc agcgtcgcat ccggtattta
     3181 ttctgctcca gcgcaaaaag gccatcctga cggatagcct tctgcttaat ttgatgcctg
     3241 gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac
     3301 ttctgagttc ggcatggggt caggtgggac caccgcgcta aggccgccag gcaaattctg
     3361 ttttatcaac acgtctcgtt atgacatgtc gatttaatct gtatcaggct gaaaatcttc
     3421 tct
//
LOCUS       DAZKJR010000264         3420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKJR010000264 DAZKJR010000000
VERSION     DAZKJR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.265"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..97)
                     /locus_tag="ACHOWS_005098"
     CDS             complement(<1..97)
                     /locus_tag="ACHOWS_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050522.1"
                     /translation="MTSREARLGMFLASLLRRIAFSYYDYKAYNFN"
     gene            385..1701
                     /gene="shiA"
                     /locus_tag="ACHOWS_005099"
     CDS             385..1701
                     /gene="shiA"
                     /locus_tag="ACHOWS_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFQ0050523.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1803..3257
                     /gene="amn"
                     /locus_tag="ACHOWS_005100"
     CDS             1803..3257
                     /gene="amn"
                     /locus_tag="ACHOWS_005100"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFQ0050524.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          804 a          832 c          815 g          969 t
ORIGIN      
        1 tattgaaatt ataagcttta taatcgtagt aactaaatgc aatacgtctc aacaatgatg
       61 ctaaaaacat acctaacctc gcctccctac tggttataat gcaatgcagt ctatcagact
      121 catcagggtg ccattttgtg catatgcgga cttttatgtt tcatatctcg aacctgtgag
      181 gcctctgctt aatccttaaa caacaccagc aactcctgcg ctttcatctt ccagcgaatt
      241 tttcatgttg ctgctcatca gccataaaat catttgcaga tgcgctctgt cgaggtagtc
      301 tcataaggtt cgtttataga tcgacggcaa tgtgagttac cttttccata ctaattataa
      361 aaagacagta caaacaggat cattatggac tccacgctca tctccactcg tcccgatgaa
      421 gggacgcttt cgttaagtcg cgcccgacga gctgcgttag gcagtttcgc tggtgccgtc
      481 gtcgactggt atgatttttt actctatggc atcaccgccg cactggtgtt taatcgcgag
      541 tttttcccgc aagtaagccc ggctatggga acgctcgccg catttgccac ctttggcgtc
      601 ggattcctgt tccgtccgct cggcggtgtc attttcggtc actttggcga ccgactggga
      661 cgtaagcgca tgttaatgct gaccgtctgg atgatgggca tcgcgacagc cttgattggt
      721 attcttcctt cattctcgac cattgggtgg tgggcaccta ttttgctggt gacactgcgt
      781 gccattcagg gatttgccgt cggcggcgaa tggggaggcg cggcgttgct ttccgttgaa
      841 agtgcgccga aaaataaaaa agccttttac agtagcggtg tacaagttgg ctacggtgta
      901 ggtttactgc tttcaaccgg actggtttca ttgatcagta tgatgacaac tgacgaacag
      961 tttttaagct ggggctggcg cattcctttc ctgtttagca tcgtactggt actgggagca
     1021 ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca acataatcaa
     1081 gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc cggtgctttc
     1141 ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt tactgccttt
     1201 gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct taatattggt
     1261 ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct tgccgatcgt
     1321 tttgggcggc gcagggttta tatcacaggt gcgttaatcg gaacgttgag cgcatttcct
     1381 ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc catcatgctg
     1441 gcaaacattg cgcatgatat ggtggtgtgt gtacaacaac cgatgtttac cgaaatgttt
     1501 ggtgccagtt atcgctatag tggcgctgga gtcggttatc aggttgccag tgtggttggc
     1561 ggtggattta caccttttat tgccgctgca ctcatcactt actttgccgg gaactggcat
     1621 agcgtcgcca tttatttgct ggctggatgc ctgatttccg caatgaccgc tttgttgatg
     1681 aaagacaatc aacgcgctta atagcctggc gaagatcatc cgatcttcgc cttacacttt
     1741 tgtttcacat tcctgtgaca tactatcgga tgtgcggtaa ttgtatggaa caggagacac
     1801 acatgaataa taagggctcc ggtctgaccc cagctcaggc actggataaa ctcgacgcgc
     1861 tgtatgagca atctgtagtc gctttacgca acgccattgg caaatatatt acaagtggcg
     1921 aattacctga tgaaaacgcc cgcaaacaag gtctttttgt ctatccatca ctgaccgtaa
     1981 cctgggacgg tagcacaacc aatcccccca aaacgcgcgc atttggtcgt tttacccacg
     2041 caggcagcta caccaccacg attactcgcc ctactctctt tcgttcgtat ctcaatgaac
     2101 aacttacgtt gctgtatcag gattatggtg cgcatatctc ggtgcaaccc tcgcagcatg
     2161 aaatccctta tccttatgtc atcgatggct ctgaattgac acttgatcgc tcaatgagcg
     2221 ctgggttaac tcgctacttc ccgacaacag aactggcgca aattggcgat gaaactgcag
     2281 acggcattta tcatccaacg gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg
     2341 atttttccct cgcgcggttg cgccattata ccggtacgcc agttgaacat tttcagccgt
     2401 tcgtcttgtt taccaactac acacgttatg tggatgaatt cgttcgttgg ggatgcagcc
     2461 agatcctcga ccctgatagt ccctacattg ccctttcttg tgctggcggg aactggatca
     2521 ccgccgaaac cgaagcgcca gaagaagcca tttccgacct tgcatggaaa aaacaccaga
     2581 tgccagcatg gcatttaatt accgccgatg gtcagggtat tactttggtg aatattggcg
     2641 tgggaccgtc aaatgctaaa accatctgcg atcatctggc agtgctacgc ccggatgtct
     2701 ggttgatgat tggtcactgt ggcggattac gtgaaagtca ggccattggc gattatgtac
     2761 ttgcacacgc ttatttacgc gatgaccacg ttcttgatgc agttctcccg cccgatattc
     2821 ctattccgag cattgcggaa gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg
     2881 gcaggcctgg tgaagaagtc aaacagcggt tacgtactgg cacggtggta accacagatg
     2941 acaggaactg ggagttacgt tactcagctt ctgcacttcg ttttaactta agccgggccg
     3001 tagcaattga tatggaaagt gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat
     3061 acgggacact actgtgtgtt tcagataaac cgttacatgg tgagattaaa cttcccggcc
     3121 aggctaaccg tttttatgaa ggcgctattt ccgaacacct gcaaattggc attcgggcga
     3181 tcgatttgct gcgcgcagaa ggcgaccgac tgcattcacg taaattacga acctttaatg
     3241 agccgccgtt ccgataataa gttatgaacc ttcttatcag aaatagatgt gaaggattaa
     3301 gtaagacatc tggcaaatag cctgcaataa acgctggctg tttgcttcct tgggcagatg
     3361 cgagttttat tatcgtctta atgatttcca catattaaaa gcaagtatgc tttcaaaaca
//
LOCUS       DAZKJR010000265         3349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKJR010000265 DAZKJR010000000
VERSION     DAZKJR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.266"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..196
                     /locus_tag="ACHOWS_005101"
     CDS             <1..196
                     /locus_tag="ACHOWS_005101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050525.1"
                     /translation="ASFKLQPIQEFSLTHLQLFPFFINVKKQLCFGIKRKTPEDMSVT
                     VLLSPLPLPWTVNAYHPYLS"
     gene            171..1382
                     /gene="bluF"
                     /locus_tag="ACHOWS_005102"
     CDS             171..1382
                     /gene="bluF"
                     /locus_tag="ACHOWS_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFQ0050526.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELMCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
     gene            1597..2328
                     /gene="bluR"
                     /locus_tag="ACHOWS_005103"
     CDS             1597..2328
                     /gene="bluR"
                     /locus_tag="ACHOWS_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFQ0050527.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2549..2953
                     /locus_tag="ACHOWS_005104"
     CDS             2549..2953
                     /locus_tag="ACHOWS_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFQ0050528.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRVAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTKKNPAKIKEAALLHISGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            3006..3116
                     /locus_tag="ACHOWS_005105"
     CDS             3006..3116
                     /locus_tag="ACHOWS_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050529.1"
                     /translation="MEIKVQRLSLWMINTVFLLPPINNHQANTINLIFEM"
BASE COUNT          997 a          696 c          686 g          970 t
ORIGIN      
        1 tgcatcattt aaattacaac ctatacaaga attttcgcta actcatcttc aactttttcc
       61 attttttatc aatgtaaaga aacagttatg ttttggtatt aaaagaaaga caccggaaga
      121 tatgtctgtt accgtcttac tctcgcctct cccattaccc tggacagtta atgcttacca
      181 cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc gaagaaatgg
      241 tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta ctgtttaatg
      301 gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg atatatcggg
      361 ctatatgcca ggatccacgg cactataata ttgttgagct gatgtgcgat tacgcgcctg
      421 ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac gagcgagatg
      481 acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact tatgatgaca
      541 gagcgctaca attttttcgt acttttgtcc ttgcaaccga acaagcaacc tatttcgaga
      601 tccctgccga agactcctgg ctttttatcg ctgacggatc tgataaagaa cttgattcct
      661 gtaccctttc accaaatata aacgaccact ttgcctttca tcctattgtc gatcccttat
      721 cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagacagc ccgtcagcta
      781 tagcggttgg gcagcgtaaa gaaggggaaa tctatacagc ggatctcaaa agtaaggcgc
      841 ttgcattcgc gatggcacac gcacttgacc tcggtgataa aatgatttca atcaatctat
      901 tacctatgac cctggttaac gaacctgacg cagtctcttt tttacttgat gaaataaagg
      961 ccaatgctct ggtgcctgaa caaatcatcg ttgaatttac tgaaagtgaa gtcatatctc
     1021 ggtttgatga gtttgccgaa gcggttaaat cgctaaaggc tgctggtatc agtgtagcaa
     1081 ttgatcattt tggcgcaggt tttgccggtt tgttactcct gtcacgcttc cagcctgaca
     1141 gaattaaaat cagtcaggaa ttgattacca atgtccataa aagtgggcca cggcaggcaa
     1201 ttattcaggc gatcataaaa tgctgtacat cacttgaaat tcaagtctct gctatgggtg
     1261 tgacaacacc agaagagtgg atgtggcttg aatctgcagg aattgagatg tttcagggag
     1321 atctgtttgc gaaagctaaa ttgaatggta tcccttcagt tgcgtggcca gagaaaaaat
     1381 aattttcagc acattatttc acatgatttc acatgatttc cgtaaatcat cgtgaaaatg
     1441 aatacgttat gtttaaattt aatccacatt aaaaataatg tttcattcaa tttagctgaa
     1501 atcttattta tactttgtac aattttagcg tatattgtac aaagtattat tgggtcgtgt
     1561 acaggcgacg gagatttgtg accgcaagga ggaattgtgg cttattacag cattggtgat
     1621 gttgctgaac gttgcgggat taatcctgtc actctccggg cctggcaacg ccgctacggt
     1681 ttgttaaaac cacagcgcag tgaaggcgga caccgactct ttgatgaaga agacatacaa
     1741 cgcatcgaag agatcaagcg ttggataagt aatggcgttc ctgtaggcaa agttaaagca
     1801 ttactggaaa ccaccagcca ggatacggaa gatgactgga gtcgcctgca agaagagatg
     1861 atgtcaattc ttcgtatggc taatcctgcc aaactacgcg cgagaattat ttcactgggt
     1921 cgagagtacc cagtagatca attgattaat catgtttacc ttcctgttcg ccagcgtctc
     1981 gtgcttgatc acaacacctc ccgcattatg agcagtatgt ttgacggcgc attaattgaa
     2041 tacgcagcag cctcactttt cgaaatgcgc cgtaagcccg gtaaagaagc cattctgatg
     2101 gcgtggaatg ttgaagagag agcacgattg tggctggaag catggcgttt atcattgtca
     2161 ggatggcaca tttctgttct tgctgatccc attgaatcgc cgcgcccgga actgttcccg
     2221 acgcaaacat tgattgtctg gacaggcatg gcaccaacga gaaggcaaaa cgaactttta
     2281 caacactggg gtgagcaagg ttataaagtc atctttcatg ccccctaatt tcacctttat
     2341 cccgcccctt agacagaatc ttataatctg aacccacaac agcttttgta ccggagctgg
     2401 tccgttgcaa aatgctcacc gggagaattc aggtaatccc cctttcacac tttgtacata
     2461 tcacagccaa aggacagacc agattgaagt ggtaaaaccg atcgtgcaca ctacgcagtc
     2521 tcacaactaa caagaggtta ttatcctgat ggatcaggtc gttgtttttc aaaagatgtt
     2581 tgagcaagtg agaaaagagc aaaacttctc ctggttttat tcagaattaa aacatcaccg
     2641 cgttgcacat tacatttatt atctggctac ggataacatc agaattatta ctcacgatga
     2701 cacggttttg ttattaagag gaaccaggaa cctgttaaaa gttagtacga aaaagaaccc
     2761 tgctaaaata aaagaggccg cattgcttca tatttccgga aaatctacat ttcgggagta
     2821 ctgttcaaca ctggcagacg cgggcgtttt ccggtgggtt actgatgtta atcataacaa
     2881 acgcagttac tacgccattg ataatacgct tttatatatt gaagatgtag aaaataataa
     2941 accattaatc tagctaaagt tggatgctta aaaaatgctt cataattcag taagacatta
     3001 gcataatgga aataaaagtg cagagactat ccctatggat gataaatact gtctttttat
     3061 tgccacccat aaataatcac caggccaata ctatcaactt gatatttgaa atgtgatcac
     3121 ttgactttct atacgttatt ttataacggt taacatattt ataaaaccaa cgggcgtgcc
     3181 atacgcccgt ttcaatactt aacgcacatg tgttttggtt tagtcatcat ccgattatat
     3241 gtattttagt caggaacggg tttaaccatt cctatataac tcaaaaattg aaaccttatt
     3301 ctcatgtcat gcttatattc attattatcg ttatataaaa atgcaacca
//
LOCUS       DAZKJR010000266         3347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKJR010000266 DAZKJR010000000
VERSION     DAZKJR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.267"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..768
                     /gene="ompT"
                     /locus_tag="ACHOWS_005106"
     CDS             <1..768
                     /gene="ompT"
                     /locus_tag="ACHOWS_005106"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFQ0050530.1"
                     /translation="DWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDW
                     MDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWFLNEPNYRLGLMAGYQESRYSFTAR
                     GGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSG
                     WVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRVTNKK
                     GDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1000..1668
                     /locus_tag="ACHOWS_005107"
     CDS             1000..1668
                     /locus_tag="ACHOWS_005107"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0050531.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1750..2092)
                     /locus_tag="ACHOWS_005108"
                     /pseudo
     CDS             complement(1750..2092)
                     /locus_tag="ACHOWS_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2105..2700
                     /locus_tag="ACHOWS_005109"
                     /pseudo
     CDS             2105..2700
                     /locus_tag="ACHOWS_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3184..>3347
                     /locus_tag="ACHOWS_005110"
     CDS             3184..>3347
                     /locus_tag="ACHOWS_005110"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HFQ0050532.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEG"
BASE COUNT         1042 a          737 c          707 g          861 t
ORIGIN      
        1 gactggaaat tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
       61 cagatatcta tcggggctgc tggctggaca acactcggca gccgaggtgg caatatggtc
      121 gatcaggact ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct
      181 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gttcctcaac
      241 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
      301 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
      361 ttcccgaatg gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc
      421 ttgactggaa gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc
      481 tgggtggaag catctgataa cgatgaacac tatgacccag gaaaaagaat cacttatcgc
      541 agtaaagtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaacg
      601 cctaatgcaa aagtttatat tgaaggcgca tggaatcggg ttacgaataa aaaaggtgat
      661 acttcacttt atgatcacaa tgataacact tctgactaca gcaaaaatgg tgcaggcata
      721 gaaaactata acttcatcac tactgctggt cttaagtaca ccttttaaca acgttaaata
      781 gattttctcc ccggtggtat tgtatagccc cggggaattt gtttaccgtt aaaagcaaac
      841 aatatcccct aatcttgtaa gcatttaacc tgcgtcctgg cgtgtaacct cttaccgcaa
      901 cacaagactc aatatattca aattcgttat gccgtggcat catttaaccc cttgtaattc
      961 atcgctataa ttgatttaat tcacaaataa aactataaca tggtgaaact aatgaaaaaa
     1021 aacacagatg atggggctaa aatttacaca ccacttaccc taaagcttta tgactggtgg
     1081 gttttgggtg tatcaaatcg gcttgcatgg ggatgtccta caaaggaaca ccttcttcca
     1141 cactttctgg aacatttagg taacaaccat ctggatattg gcgttggaac tgggttttac
     1201 cttactcacg tacctgagag tagtctgata tctttaatgg atttgaacga agctagcctg
     1261 aacgcggcat cgacaagggc tggggaatca aaaattaaac ataaaattag ccatgatgtt
     1321 tttgatcctt atcccgcggc gttacatggt caatttgatt ccatttccat gttttacctt
     1381 cttcactgcc tgcctggaaa tatatctaca aaaagctgtg taatacgcaa tgcggcgcag
     1441 gccttaactg acgatggaac tctatacgga gccacaattc ttggtgacgg cgttgtgcac
     1501 aatagcttcg gtcaaaaact gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca
     1561 aaagattccg aagaaggctt aacacatata ctctcagagc atttcgagaa tgttaaaacc
     1621 aaggttaaag gtactgtagt aatgttttcc gcttcaggga aaaaatagca tccagccgca
     1681 gcacgttctt gcgcacgacg ttctgcggca taatcccaat gattactctc tgacagggtt
     1741 cacaggccac taaatatcag gggcagttga tgtatcaact cggttcagca acacccgata
     1801 catcttccag gcttccagca acgaggtttc ttcctccgta tcctttatcc atgatatgcc
     1861 gttccacttc tgaaactccc ctgccgggga tacctggtga cattttccgg taacggaccg
     1921 agttcagaaa taaataatgc gtcccctgat gccacgtcat aaaccgtttt accccgatgg
     1981 tcttcaacga gatgccacga tgcctcatca ctgttgaaaa caaccacgaa gccagccgga
     2041 atatctgggc aatatcagta ctgtttgcag gcaaacctgt atgaggtgga atggcacaag
     2101 agacttacaa aagaatcagg ccaaacgcct gattgatgag agccccgaac cactgaaatc
     2161 tgtcgtcaaa tttgcgctgg taactgttct gagaaagtcg aacatcataa atctggagtg
     2221 gcaacagatt gacatgcagc gacaagttgc cagggtgaat ccagaagata gcaaatcaaa
     2281 ctgcgccatt ggtgtggcgc tgaacgatac cgcctctaaa gtgttgcgtg atcaaacagg
     2341 caagcatcac aaatgtgggt gtacatacca gggcggctaa gcgagcagat ggaacatcaa
     2401 tgcctgcgct caggaagatg cgcattgaca gcaagacagc atggctatca gcttgtcgtc
     2461 gtgcaggaat tgaaaatttc cgtttccatg acctctggca tacctgggca agctggttga
     2521 ttccgtcagg cgtcccatta tcagtgcttc aggaaatggg cggatgggag tccagagaaa
     2581 tggttcgtag atatgctcac cttgcgccta atcatttgac tgagcacgcg aggaaaatag
     2641 acgacatttt tggtgataat gtcccactct ggaattatag aagaaataaa gaaggcgtaa
     2701 ctgattgaat tttaatggcg cgccctgcag gattcgaacc tgcggcccac gacttagaag
     2761 ttcctagaac gacattttaa gtcaacaact taccgcgcca tctctgcgct cacacgtccc
     2821 actacctcaa aacatgtaaa gccttgcaag ccattgcgag gccttatgtg tctcagtttt
     2881 gtccctcttt tttgtactaa aaaacatagt aattgaggat aaacctcatg ctattttcgc
     2941 ttatatgcct ctaaaggcat ggcacttaaa tagataaaag caccacaaaa gcataaaaaa
     3001 accacacagt aaaaccgcaa tatgaaacaa taacagataa ttaaaccaaa aacagatagc
     3061 gcattgtgat aatcattcaa tactaaacaa aatataaaca gtggagcaat atgtaattga
     3121 ctcattaagt tagatataaa aaatacatat tcaatcatta aaacgattga atggagaact
     3181 tttatgcggg cgaaacttct gggaatagtc ctgacaaccc ctattgcgat cagctctttt
     3241 gcttctaccg agactttatc gtttactcct gacaacataa atgcggacat tagtcttgga
     3301 actctgagcg gaaaaacaaa agagcgtgtt tatctagccg aagaagg
//
LOCUS       DAZKJR010000267         3310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKJR010000267 DAZKJR010000000
VERSION     DAZKJR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.268"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(134..>3310)
                     /locus_tag="ACHOWS_005111"
     CDS             complement(134..>3310)
                     /locus_tag="ACHOWS_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0050533.1"
                     /translation="QSLVETTSKGDRNPSSVRLLIQLERNGHWVTEKDVTINGKTTSQ
                     YLTSVILNNLPERPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKVW
                     DVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKD
                     RHTAVEVNYTDPQNGWQTSTELVEDPDGILRYGRNLLKMDAFGCTSRGQAHRAGLWVI
                     KTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDRE
                     VILLEAGTSMVNLINGSGKPVRVDITAHPAPDRIQVSALPDGVETYGVWGLSLPSLRR
                     RLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPMSGSLNSVIPPAVQHLTVE
                     VSASDGQYLALAKWDTPRVVKGVRFSLRLTSGNGENSRLVTTAITADTEHRFSGLPLG
                     EYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYYQITAVPHLTVYDPTVQF
                     EFWFSEAKIADTSQVETSARYLGTGRQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVE
                     ASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQ
                     TVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQ
                     PLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGEPPTFS
                     LTPDGKLTARNADISGNINANSGTLNNVVIAENCTIKGTLRAENIIGDVVKTHNVSLP
                     ALSAAGDHRHATERTVTVIDDQSFDRNIVIPPVFYSGIVYQGSDGDGATSICTLTVSV
                     NDRVVFSKSSSIGTGRTSGGYPVQKDTMYPGSSTPFSYFMRMASGGGDVRIKFRIEVI
                     YYGTAPGFMTTGIQITTMKATASGFMES"
BASE COUNT          646 a         1018 c          868 g          778 t
ORIGIN      
        1 cgggttaaac atgaatgttt gtccggggcc ttcgtctttc cggccttcag gtgttccctc
       61 cggccatcag ccaaaggcac ccgcgcatac tgtacggttt ttgtctcctt ccggcaatca
      121 cgatcgtccg ggattaactt tccataaatc ctgaggctgt cgccttcatc gttgtgatct
      181 gtatccctgt ggtcataaaa cccggtgcag ttccataata gatgacctca atccggaact
      241 tgatcctgac atctcctccc cctgacgcca tcctcatgaa atagctgaac ggagtggatg
      301 acccgggata catggtgtct ttctgaacgg ggtatccacc tgacgtacgc ccggtcccga
      361 tggatgagct tttgctgaac actacccgat cattcacact taccgtcagc gtacagattg
      421 aagttgcacc atcaccatca cttccctggt agacaatacc ggaataaaaa accggcggga
      481 taacaatgtt ccggtcaaac gactgatcat caatgacagt aaccgttcgt tctgtcgcgt
      541 gtctgtgatc ccctgctgca ctgagagcag gaagactgac attgtgcgtt ttcaccacat
      601 cgccaatgat attttccgct ctcagagtac ctttgatggt acagttctcc gctatcacga
      661 cattattgag cgtgcccgag ttcgcattaa tattcccgct gatatccgca ttacgggctg
      721 tcagtttccc gtccggcgtc agggaaaacg tcggaggctc acctgatgag gtaatactca
      781 ccgcaaacag tcgtttcagg aacacatcgt tcatgaacag ctgattcccc tgcgccacaa
      841 acagcggcgt ggtgttgccg ttctccgggt taatcatcgc gatacggtcc gcctgcagca
      901 gtatattgct caggggctgg ccatcagcat cctcaatccc cgcacctata ccggcaacat
      961 acggaatgcc gtttttcgtt ttctgcacct tcagcatgta cagcgcagcc aggtcgttat
     1021 ttgtgtctgt ctgcacccgc tgtatctgct gtatggtggc actctggtct tccagggttt
     1081 tactgaccgt ctgcgtgatt tcattgcggg tttctgtgat ggtggtcttc atctccgcca
     1141 tctcatcatc aagctggctg ttatcaatca gctcccacat cccctgtgcc agatgtgtct
     1201 tcccgatttc tcctctgaaa aagtccagat acccttccgc atcattgctg gcccggccac
     1261 tggcttccac aaacgcagat ttccccacca ggttgacgct gcgcacataa aaccagaaat
     1321 ccttccccgg cttaatgtgc gggccggaga cactccactg gcggcctgtc cccagataac
     1381 gggcagaggt ttccacctga gatgtgtctg cgatttttgc ctccgaaaac cagaactcaa
     1441 attgtaccgt cgggtcatac accgtaagat gcgggaccgc cgttatctga taataccccg
     1501 gcgtcagttc aatgctggcc ggtgctgccg gtgcgttaat ccggaacgtg gtggtggcag
     1561 gttcgccctg ctggccgtag ctgtttatcg ccctgaccgt cagggtgtat tcccccagag
     1621 gcaggccact gaaacggtgc tccgtgtcgg cagtgatggc ggtggtcacc aggcggctgt
     1681 tttcaccgtt tccgctggtc aggcgcagac tgaagcgcac gcccttcacc acccgcggcg
     1741 tgtcccattt cgccagtgcc agatactggc cgtctgaggc actcacctcc accgtaaggt
     1801 gctgcaccgc cggcgggatg acgctgttca gtgagccgga catcggctca aagcgggccc
     1861 cgttatccac aatggcttct ttttccggca catgctgcac cgccgtgatg gcaaaggtac
     1921 cgtccgtgtt ttcccggatg gaaacacagc ggaacaggcg acgacgcagt gacggcaggg
     1981 agagtcccca cacaccgtat gtctccacgc catccggcag ggcgctgacc tgtatccggt
     2041 caggggcggg gtgtgcagtg atgtccacgc gcaccggctt accgctgccg ttaatcaggt
     2101 tcaccatcga tgtccctgct tccagcagta tcacctcacg gtccagcgtc agggtacggc
     2161 tggcggcatc gatggacagg atgcgtccgc cggtcagggt cccggcatag tcgttatcac
     2221 agatttcaat gatgtcaccg ggtgtgtgac gcagcccctg tgacccgagc gtgaaatcca
     2281 ccgtctgcgt ttccagcagt tcggtcttta tcacccacag tccggcacgg tgggcctgac
     2341 cgcggctggt acagccgaac gcatccatct tcagcaggtt gcgcccgtag cgcaggatgc
     2401 cgtccgggtc ttccaccagt tccgtggaag tctgccagcc gttctgcggg tcggtgtaat
     2461 tcacctccac cgccgtgtgc cggtccttca gggcactgaa gctgtagcgg aaccccacgc
     2521 cgttatcatc caccaccaca tcgctgttgg tgtagggcca caccacatcc gacgggcggt
     2581 cctgaacgaa cgtcagcgtc tggccgttcc ataccggcat acagcgcatc gccgagcaga
     2641 aatcccccag gacatcccag accttacgct gctgtgacag gtacgcatta aaagtcatcc
     2701 gcggctctgt gcccccgaaa ccatccggga ccgtctggtc acagtactgc ccgatggcat
     2761 acagtgccca cttgtccaca tccgcggccc ccaggcgttt tcccatgccg tagcgcgggt
     2821 gggtcagcat gtcccacagg caccaggccg ggttattgct gtatgccggt ttcagactgc
     2881 cgtcccagat accgctgtag gtgcgttttt ccgggtcata gtttgacggc acctgaatga
     2941 tgcgaccacg gatatggtag ttcaccgtca gctgctggcc accaaactgc tccgcatcca
     3001 cctgcagccc cacaatggcc gtgttcgggt agcactgttt cacatcgatg atttcggtgt
     3061 atgacgacca cagcgttctg ttctgcagct ggtccgtggt gctgtccgcc gtctccctga
     3121 ccatccggat gttaaagggg cgctcaggga gattattcag aatcaccgac gtcaggtact
     3181 gcgaggtggt cttgccgtta atggtgacat ccttctccgt cacccagtgc ccgttacgct
     3241 caagctgaat cagcaggcgg acagaagagg gattacggtc accctttgag gtggtctcca
     3301 ccagtgactg
//
LOCUS       DAZKJR010000268         3289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKJR010000268 DAZKJR010000000
VERSION     DAZKJR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.269"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..208
                     /locus_tag="ACHOWS_005112"
     CDS             <1..208
                     /locus_tag="ACHOWS_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050534.1"
                     /translation="FTDAVGHEFMVESHLITTTTPCPSDADYLYIHLADGTQITAIAS
                     TVREVTAIRGAWKSETQAHGELRP"
     gene            205..417
                     /locus_tag="ACHOWS_005113"
     CDS             205..417
                     /locus_tag="ACHOWS_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050535.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            444..998
                     /locus_tag="ACHOWS_005114"
     CDS             444..998
                     /locus_tag="ACHOWS_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFQ0050536.1"
                     /translation="MRLTPMGTPGKAPAHVKPWTQQEDRLLISLYQEHNTHQFAERIQ
                     RTRKAVMHRIRFLRNRGLIGRKRKAPLSTEVIAFLIKNRHVKTAREMALEAGCSECTV
                     RYTLRKRGYNLQKCGENHHCARYSDHLVELVTELRDIHNMTFCMIAEHINSTTQMHIN
                     DDTAFHLYHRRTATDAVLRELLPG"
     gene            1008..1631
                     /locus_tag="ACHOWS_005115"
     CDS             1008..1631
                     /locus_tag="ACHOWS_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050537.1"
                     /translation="MHTQKNRLPAFAWSGYSFPAPHKTGAGISTPPITKAHNRASGFF
                     VRTVLPRLFRVRIMAGRTGPTSVGPGSLVTGCGNPVRLATPSFPPLDGEFSKFTTKEA
                     TPWQTANNSAPALSVFTSALKLTADSIGLHLSPSSCSQTYYTDLTTVSLPGITRQSSA
                     IWPKTWQNCRTLSTSSNTTTKQHPEAIPHPCAGGFRTSVTGGSHEER"
     gene            1618..2034
                     /locus_tag="ACHOWS_005116"
     CDS             1618..2034
                     /locus_tag="ACHOWS_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050538.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDGRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMTLVLEMTTHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQATTAGECDA"
     gene            2031..>3289
                     /locus_tag="ACHOWS_005117"
     CDS             2031..>3289
                     /locus_tag="ACHOWS_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFQ0050539.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVDFLTNTDEGKRINSHLHHDEERRIRLEKLTRHKRENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSTALERACDEVIEKTG
                     RGDDDLLTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTDPDPERVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMACVRGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRF"
BASE COUNT          813 a          973 c          867 g          636 t
ORIGIN      
        1 tttcaccgat gccgtgggcc acgagttcat ggtggaaagt catttgatca caacgaccac
       61 gccgtgcccg tcagacgctg attatctcta catccatctg gctgacggca cacaaattac
      121 cgcgattgcc agtaccgttc gcgaagtaac ggccatcaga ggggcatgga aatcagaaac
      181 acaagcccac ggagagctgc ggccatgatc agcaagtaca ccaccaacgc acgtaaaccc
      241 ggtaaagacg cacgccacca catcgccgta ctccgcaatc tgttactgga ggaaatcgac
      301 gacttcacgg cgacgctgag cggtccgggc gcaccagtaa caccaggtga gatccaccgg
      361 gaccttaaac gccgtattga aaacgccatt gattacaccc tgaacccgac cgaataaccg
      421 gaacagtaac ggaggttatc gcgatgagac ttacaccaat gggaacgcca ggcaaagctc
      481 cggcacatgt aaagccgtgg acacaacagg aagatcggtt actcatctcg ctttatcagg
      541 aacacaacac acatcagttc gccgaacgaa ttcagcgaac ccgtaaagca gttatgcacc
      601 ggattcgttt tttacgtaat cgtgggctga ttggccgcaa gagaaaagca ccactgagta
      661 cagaagtcat agcctttctt attaaaaacc gtcatgtaaa aaccgcacgg gaaatggctc
      721 tggaagcggg atgcagtgag tgcacagtga ggtacacact gcgtaaacgg ggttacaacc
      781 tgcaaaaatg cggtgagaac caccattgcg ccagatacag cgatcatctg gttgagctgg
      841 tcacggagct acgcgacata cacaacatga cgttctgcat gatcgctgaa cacatcaaca
      901 gcacgacaca aatgcacatt aacgacgata cggcgtttca tctctaccac cgccgcaccg
      961 caactgacgc agtgctccgt gaactgttac cgggctgagt gatcatcatg cacacacaaa
     1021 aaaaccgctt gccagcgttc gcctggtcgg gttacagttt tcccgcacct cataaaacgg
     1081 gtgccgggat ttccaccccg ccgataacca aagcgcacaa ccgcgccagc ggtttttttg
     1141 tgcgtactgt attgccacgt ctttttcgcg tcagaattat ggcggggcgt acggggccga
     1201 cttcggtcgg gccgggttct ttggttaccg gttgtggaaa ccctgtacgc ctcgccaccc
     1261 cgagttttcc acctctggat ggtgagtttt caaaattcac aaccaaagag gccacaccat
     1321 ggcaaaccgc aaacaacagc gcgcccgcgc tgagcgtctt cacatccgca ctgaaattaa
     1381 ccgcagactc catcgggctg catctgtctc cttcatcatg cagtcaaacg tattacaccg
     1441 acttaacgac ggtatcgctg ccgggtatta cgcggcagtc ttcagctatc tggccgaaga
     1501 cctggcagaa ctgcaggacc ttatcaacca gcagcaacac caccactaaa caacatcctg
     1561 aagccatccc gcacccgtgc gcgggaggct tccgcacgtc tgttaccgga ggttctcatg
     1621 aagagcgtta agggactttc agatattcag gattttttta actggatgtg ggcagtggat
     1681 ccccggctgg cttcccgcct gaacgactgg cacgactatt accgccgcgc ctgtaaacag
     1741 gccgccagac gcgtcaccac atacggggat ttctccttca ccgttgacgg ccgttaccgc
     1801 gtggacgtga aaggcgatgg tgatggtttt ggtttatttc acatcaacgg aaccgacagc
     1861 accgccatca gctggtcgtc gtcagagatg acgcttgttc tggaaatgac aacgcacagc
     1921 gtggccgtga aaggcatata caccgcagaa gactttatcg gtgaatacca gcgccttctc
     1981 gtgctgtacc gcgcggcgtt tcaggcgacg actgccgggg agtgcgacgc atgagtgacc
     2041 acaatgcaca gctggcgtac ccgtggaacg ccccgctacc ggttatcgat cctgaaacgt
     2101 tcggcagagc gcagccaacc gcattacgcc aggccattca gagctacatc aacgaggata
     2161 ttcgcactga cgccagactg gatgaagaaa ccgtcgactt cctgacaaac acagacgaag
     2221 gcaagcgaat taattcgcat cttcatcacg acgaagagcg tcggatcaga ctggaaaaac
     2281 tgacgcgaca caaacgcgaa aatccaccca cgctggtatc tgaagccatg gcagagctgc
     2341 gtgccctgcc accgtttctt gccggtccgc tcatccgtga cctggcccgc ctgagccgca
     2401 aacaggagag cgcacgccgg gaagggctta agaataacag cgcatatgcg gcggataact
     2461 tcgttcgtca cgggctgcgt gcccgcctga aacgtatcgc ccgtgtgaat gaacgctttg
     2521 ccaccccggc ctttaaggcc accgccgcgc gcgaacggct ggatgaactg cttatgctgc
     2581 cacagctgag ccgtgacgaa atccagcgcc ttgccaccct gaccgccacc gcgttttcca
     2641 ccgcactgga gcgcgcatgt gatgaagtca tcgaaaagac cggcaggggt gacgatgacc
     2701 tgctcacctg gttactgacc taccagccac tggcacacat ggcgttaaaa ctgggtatca
     2761 cgccaccctg ctggccgtcg ctggaaatca ggcccgaccg ccgcaccgac cccgacccgg
     2821 aacgggtgcc gggtgcggtc atgcgccttt cctgtgcgac atggtggaac agtcagttaa
     2881 gacacctccg ggacatctgg cgtgaagagc tgttacgcgc cgcaggcctg gtgtcacgta
     2941 agacgtcggt atatatcagc catgaagcgt tactggactg gcgcgaaaaa cgccagcgca
     3001 cccgcgattt tctgaaggcc tgggacatcg agaacgaaga gggagaacgg ttcagccttg
     3061 aggatatcta ctgggcaggt ctctctaatc cgcgtaaccg ccgtaacgag atgatggcct
     3121 gcgtccgggg gatggagcag gtcgccgaag cacgcggcga cgtggcgttt ttcgtcaccg
     3181 tcacagcccc ttcccgtttt cacagcgtga acgatgacgg cagcctgaac ccgaaatact
     3241 gcggagcaac ggtacgcgat gccagtgatt atctggttta ccgcttttt
//
LOCUS       DAZKJR010000269         3200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKJR010000269 DAZKJR010000000
VERSION     DAZKJR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.270"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            109..627
                     /locus_tag="ACHOWS_005118"
     CDS             109..627
                     /locus_tag="ACHOWS_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFQ0050540.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            837..2945
                     /locus_tag="ACHOWS_005119"
     CDS             837..2945
                     /locus_tag="ACHOWS_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFQ0050541.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQVIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            3012..>3200
                     /locus_tag="ACHOWS_005120"
     CDS             3012..>3200
                     /locus_tag="ACHOWS_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050542.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETD"
BASE COUNT          796 a          818 c          932 g          654 t
ORIGIN      
        1 aatactgaaa aatgatgcgg ttccgtgaag agagctgcat ctgcctgtct gggattaacg
       61 gcaggtcatt gtaagtagta attaaccacg ttaacaagga gtttacaaat gccaacaccg
      121 tgttatatct ccatcactgg tcagacccag ggaaatatta ctgcaggtgc atttaccgct
      181 gattctgtcg gcaacatcta tgtgcagggg cacgaagacg agatgctggt gcaggaattc
      241 ctgcataacg ttactgtccc gaccgatcct cagtccggcc agcctgcggg tcagcgtgct
      301 cacaagccgt tcatttttac cgtggcgctg aacaaagctg ttccgctgat gtacaacgca
      361 ctggcctccg gcgaaatgct gccaaccact gaacttcact ggtggcgtac ctctgtggaa
      421 ggcaaacagg agcattattt caccacccgc ctgaccgatt cgacgattgt cgatatgaaa
      481 ctgcatatgc cgcattgcca ggatccagcg aaacgcgagt tcacccagtt gctcgaagtt
      541 tcactggcct accgcaaaat agagtgggaa cacgtcaagt ctggcacctc cggtgccgac
      601 gactggcgcg caccgctgga agcataagtt aagccaacag catccggcta tcaggccgga
      661 tgctgttttt gtattacgcc gtaaaaagaa aggttgtcag taagggatta cgttcacggg
      721 agatggcgga tgccggaaac ttcggcggta gcgtgcctga acgcaagcgt aacactgtac
      781 tgttacagcc gctttaacag cgaactgtct gtttttcctt aaacccggag gttgctatgt
      841 caaccggatt acgtttcacg ctggaagtgg acggtctgcc accggacgcg tttgcggtgg
      901 tctcctttca tctgaaccag tcactctctt cgctcttttc cctcgatctc tctctggtca
      961 gccagcagtt tctctccctt gaatttgcgc aggtgctgga caaaatggcc tacctgacgg
     1021 tatggcaggg cgatgacgta cagcgccggg tgaaaggtgt ggtgacctgg tttgaactgg
     1081 gggagaacga caaaaaccag atgctgtaca gcatgaaggt gtgcccaccg ctgtggcgca
     1141 cagggctgcg ccagaacttc cgtatcttcc agaatgagga catcgaaagc atactcgcga
     1201 cgatcctgaa agaaaacggg gtgaccgagt ggagtccgct gttcagcgag ccgcatcctt
     1261 cccgtgagtt ttgtgtccag tacggcgaaa ccgattacga tttcctgtgc cggatggcgg
     1321 cggaggaagg catcttcttt tatgaggagc acgcgcaaaa aagtaccgac cagagcctgg
     1381 tcctgtgcga caccgtgcgt tatctgccgg agtcctttga gatcccctgg aatccgaaca
     1441 cccgtaccga ggtgagcacc ctatgcatca gccagttccg ctacagcgca caaatccgcc
     1501 catcttccgt ggtgaccaaa gactacacct ttaaacgacc cggctgggca gggcgttttg
     1561 atcaggaagg ccagcaccag gattaccagc gcacacagta tgaagtgtat gactaccccg
     1621 gacgtttcaa gggtgcccac gggcagaact ttgcccgctg gcagatggat ggctggcgaa
     1681 acaacgcaga agtggcgcgc ggaacaagcc gttcgccgga gatatggccg ggacggcgaa
     1741 ttgcgctgac ggggcatccg caggcgaacc tgaaccggga atggcaggtg gtggcaagtg
     1801 aactgcacgg cgagcagccg caggcggtgc cgggacgcag gggttcaggt accacgctga
     1861 ataaccactt tgcggtaata ccggcagaca gaacatggcg gccacagccg ttgctgaaac
     1921 cgctggtgga cggcccgcag agcgccgtcg tgacgggacc ggcaggagaa gaaatcttct
     1981 gtgatgaaca tggccgcgtg cgggtgaaat ttaactggga ccgctataac ccgtcaaacc
     2041 aggacagttc atgctggatc cgtgtggcac aggcgtgggc aggcaccggc tttggcaacc
     2101 tggcgatacc gcgtgtgggt caggaagtca ttgtggactt cctcaacggc gatccggacc
     2161 agccgattat tatggggcgt acctaccacc aggaaaaccg cacccccggc agcctgccgg
     2221 gaacaaagac gcagatgacc attcgttcga aaacctataa gggcagcggg tttaatgaac
     2281 tgaagtttga cgatgcgaca gggaaagaac aggtctacat ccacgcgcaa aagaacatga
     2341 acaccgaggt gctgaataac cggaccacgg atgtgataaa caaccatgct gagaccatcg
     2401 gcaacaatca ggtgattgcg gttaccaaca atcagataca gacggtgggc gttaaccaga
     2461 tagagacggt gggcagtaac cagatcatta aagtgggctc tgttcaggtg gaaacgattg
     2521 gacttgttcg tgcgctgacc gtgggcgtgg cgtatcagac gacggtaggt ggcattatga
     2581 acacctcggt ggccctgatg cagtcctcgc agatcggttt gcataaatcg ttgatggtcg
     2641 ggctgggtta tgacgtcaaa gtcggaaata acgttacctt caccgttggt aaaacgaaaa
     2701 aggatgatac cggacagacc gcgatttact ccgccggtga gcatctggaa ctctgctgtg
     2761 gtaaggcaag gctggtgctg actaaggacg gacaaatttt tctcaacggc acaaaaattc
     2821 atttgcaggg taaggagcag gttaatggtg actcactgtt gattaactgg aactgtgcag
     2881 cctcgaaatc cccaccgaag acccctgatg aaaagcagga tacgccggat atgagagagt
     2941 actgatcccg tacctgttcg gtattcagta acaaacaagc attaaaaata aacagaagaa
     3001 ggaattgaat catgagcgga aaaccagcgg cgcgtcaggg agatatgact cagtatggcg
     3061 gtcccattgt ccagggttcg gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct
     3121 cggtgtgtcc gggcgggatg acttcgggca acccggtaaa tccgctgctg ggggcgaagg
     3181 tgctgcccgg cgagacggac
//
LOCUS       DAZKJR010000270         3198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKJR010000270 DAZKJR010000000
VERSION     DAZKJR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.271"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2262
                     /locus_tag="ACHOWS_005121"
     CDS             <1..2262
                     /locus_tag="ACHOWS_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFQ0050543.1"
                     /translation="FFKKKKTKSAGSYTSAPVKAPPKEWLANNKKNQSVMQNEDSEDD
                     NLATFTLSGGRNVEYRITATQRQNFSKTTGALARWIFPGEMITVSGIEVTGGHFYLGQ
                     RMKPAGHNSGGYYDGNNEASLVDDTLKIYPTPYIYEDSSLGYWPSFSSLSPEARGAYL
                     SWLASDRCDTSCPIGYVFIYLYGLERRALVDSRDNNISDDEFYSLFKEICRLRSVFNE
                     NRSFRNYSSQLLEAMSILRPNENLAAALNDDSEFSNSMQFRLILAKTVDTGAPVPADL
                     ALTWVTNHAEYSLRTPARRCAKEFAALFKRRYTLKYGEGMVVKPNKARLRLDYTPASP
                     SLRGIRLPVPDLPDPGALKGPVQKLMAIADICTGELDAYSRYLGRKGTSANDTAAILL
                     LPSEIVNESAEKILSTFKHWADDAIRSKGGIVSVADYWSHMNATCPAKINKKEADLMQ
                     AFAQKMGYCLAPDPYHHHVKADVDGVLVLFPAGERGRFSPYPEFITAVLTLRLGSVVA
                     LIDNSLDQAEQKVLENAINNNASFSDDEKRSLHAYLTWQLHTPANMTGMKSRIELLGA
                     AEKSAVGKVIVSVACSDGRITPAEIKQLEKIYTSLGLDPSSVSSNIHQHSATETALVS
                     SVPANQSTAGFTLDANVLARHESATDDVRKLLNSIFTEEEPEQPESAPASLTGEGGLD
                     SAHSQLYRRLLEKEQWSRKEATELCSNWNLMLGGALEVINDWSYAVVDAPVLEDADDH
                     IWIDLEIAKELEG"
     gene            2266..2667
                     /locus_tag="ACHOWS_005122"
                     /pseudo
     CDS             2266..2667
                     /locus_tag="ACHOWS_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
     gene            complement(2638..2846)
                     /locus_tag="ACHOWS_005123"
                     /pseudo
     CDS             complement(2638..2846)
                     /locus_tag="ACHOWS_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2881..3015)
                     /locus_tag="ACHOWS_005124"
     CDS             complement(2881..3015)
                     /locus_tag="ACHOWS_005124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050544.1"
                     /translation="MSEIKIIGIELAKTNFYPFNINDYGKTVGKIKFSRSNLLNLLVQ
                     "
BASE COUNT          896 a          716 c          739 g          847 t
ORIGIN      
        1 tttttcaaaa agaagaaaac taagtccgcg ggctcttata cttccgctcc tgtgaaagca
       61 ccacctaaag aatggcttgc taacaacaaa aaaaatcagt ctgtaatgca aaacgaggac
      121 agcgaagatg acaatctggc tacgtttaca ctaagtggcg ggcggaatgt cgaatacagg
      181 ataacagcca ctcaacgcca gaatttctca aaaactactg gtgctttggc tcgttggatt
      241 tttccgggag agatgatcac agtcagtggg atagaggtta ccggcggtca cttctatctt
      301 gggcagcgta tgaaacctgc cgggcataac tcaggcggtt attatgatgg caataacgag
      361 gcatcgctag ttgacgacac gcttaaaata taccccacac cttacattta cgaagatagt
      421 tcattagggt attggcccag tttttcctca ctttcacctg aagctcgtgg tgcgtacctg
      481 agttggcttg ccagcgacag atgcgacact tcctgcccaa ttggttacgt tttcatttat
      541 ctgtatggtc tggaaagaag ggcgcttgtt gacagcaggg acaacaatat atccgacgat
      601 gagttctatt ctcttttcaa agagatctgc cgtctcagat cggttttcaa tgaaaaccga
      661 tctttccgga attactcatc ccagttactt gaagcgatgt ccattcttcg tccgaacgaa
      721 aatcttgcag cagctctcaa cgatgattcg gagttcagca atagcatgca gttcaggtta
      781 atcctggcaa agactgttga tacaggagcc ccggttccag cggatctggc tctgacgtgg
      841 gtgacaaacc atgctgaata ctcattacga acccccgccc gccgctgtgc gaaagaattt
      901 gctgcactat ttaaacggcg ttacaccctt aaatacggtg aagggatggt agtgaagccg
      961 aataaagcgc gtctgcgttt agattacacg cctgcaagtc ccagtttgcg aggtattcgg
     1021 cttcctgttc ccgacttgcc tgatccaggt gcattgaaag gcccagttca gaagcttatg
     1081 gccattgccg atatatgcac aggtgaactg gatgcttaca gtcgttacct tggtagaaaa
     1141 ggtacttcag caaatgatac cgctgcaatt ttgttgctgc cttcagaaat cgttaacgaa
     1201 agcgcagaaa aaatattaag tacattcaaa cactgggctg atgacgcaat ccgcagtaag
     1261 ggtgggatag tttcagttgc tgactactgg tcacatatga atgccacctg tccggcgaag
     1321 atcaataaaa aagaggccga cttaatgcaa gcctttgcac agaaaatggg atactgtctg
     1381 gctcctgacc cctatcacca ccacgtaaag gcagatgtcg atggtgtact tgtcttattc
     1441 cctgctggtg aaagaggacg tttctcacct tatcctgaat ttatcacagc tgtattgacg
     1501 ctccgattag gttcggtagt cgccttgatt gacaattctc tcgatcaagc cgaacagaaa
     1561 gtgcttgaga atgccatcaa caacaatgcc agcttcagcg atgatgaaaa gcgctctctg
     1621 cacgcatatt taacgtggca gcttcatact ccggcgaata tgacgggaat gaaaagccgg
     1681 attgaattac tcggtgcagc ggaaaaatca gctgtgggta aggttatcgt tagtgtcgct
     1741 tgctctgacg gcagaattac gcctgccgaa atcaaacagc ttgagaagat ttatacaagc
     1801 ctgggactgg atccctcatc cgtatcaagc aatatccatc agcattccgc aactgaaact
     1861 gctctcgttt catctgtacc agctaatcag tcgacggcag ggtttacgct ggatgccaac
     1921 gtacttgccc gccacgaatc tgccactgat gatgttcgta aattactgaa ctcaattttc
     1981 actgaagaag aaccagaaca accagaaagc gctccagcct cattaaccgg ggaaggggga
     2041 cttgactccg ctcatagcca actttatcgc aggttgctgg agaaagaaca atggtccaga
     2101 aaagaggcga cagagttgtg tagtaactgg aacctgatgc tcggcggtgc gcttgaggtg
     2161 attaacgatt ggtcctatgc ggtggttgat gcaccagtgc tggaagatgc tgatgatcac
     2221 atttggattg acctggaaat agccaaagaa ttagagggtt agtgaatgtc tgtaacacgt
     2281 ataagagtga aagagcgcga cgctatcatt cagtcactta agtcgggagt aacgccaaga
     2341 ataggtattc agcatatcca ggttggccgc gtgaatgaga taaccgccct ccatcaggat
     2401 attgaacgga ttgctgatgg cggggcaagc ttcagactta tcattggtga gtacggttcc
     2461 gggaaaacat tctttttaag tgttgtacgg tctattgcgt tagagaagaa gctggtcacg
     2521 gtcagtgcag acctttctcc cgacagacgt atccactcat cgggaggtca ggctcgtaat
     2581 ctctactcag agcttatgaa aaatatgtcc accagaaaca aaccagatgg caatgcatta
     2641 cttagcgttg tggaaaggtt tgttacagtc aaggcagtca ccaaactcga taataaagcg
     2701 gctcatttcc atacgtctgt ccttaagggg cattgttcat ttctgtgacc aagccgcacc
     2761 acctttttta ctgattcgtc tgtcgggaac actttgcgtt tcttgatgac ataacggatc
     2821 acactattca acgactctat ggcattttgc acatacttcc atagcaactg tcatcggtag
     2881 ctactgaacc agcaagttaa gtaagttact acgtgaaaat tttatttttc caacggtttt
     2941 tccataatca ttaatattaa aaggataaaa atttgtttta gccagctcga taccaatgat
     3001 ttttatttca gacatgatat ccttcttaca tttcattgat taaagtttat ccatcatggt
     3061 atgatctgct tctaccgtga aggaggggct gacactcaat ggaccaaaaa ctcacgttaa
     3121 acaattgtag caaaggaaag ttaaaggatc ctgcgaggat cctttgatag gtgttaaaat
     3181 tgcattctta ttaccata
//
LOCUS       DAZKJR010000271         2959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKJR010000271 DAZKJR010000000
VERSION     DAZKJR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.273"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..840
                     /locus_tag="ACHOWS_005125"
     CDS             <1..840
                     /locus_tag="ACHOWS_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFQ0050545.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKPLNEAVLFSAPEGIALT
                     SGEDVQLAAGQNLAVNAGGDISTGTMGNLTALAGERLGLFARSGQLSVKSGEGPVEVQ
                     AQNGSMRLFAEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTTVSYLRKV
                     KRTIAAGAASQQPEMPWLADPVKPSASALMQYLYHDDVPVVGAPFKATLADGTVREGN
                     LDGAGYLHLEDVPPGRVLVELGPDTRDYLRKNKTDNRLFVGTSLSEADIDSLIAKYSE
                     GVE"
     gene            837..2381
                     /locus_tag="ACHOWS_005126"
     CDS             837..2381
                     /locus_tag="ACHOWS_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050546.1"
                     /translation="MSGVNVAWFKETSLETGQWIWGTLQGAFNEKQTIGQIIVDAVIG
                     MIPLVGDVTAVRDLIAVSIGLAKEPKKRESVTEWVFLVILLFALIPVIGGVIKGVGRL
                     ALRVTTKVAENVETLGAVIKFLNRIGEGDAVKWLNKLNVVEYQAQLIEKMGDFCNTMI
                     TAINKILKARVGRMLPEKWQADLLLVRDGFAALQRLASRMIPQALKELDAKLRVLQAL
                     VYKGEIHTITTGMGRAEVRREAEAYLIERPLCEAPKQGTRYPSVHAEVIHEDRLRLKY
                     SREGYPDLLGYKGKSPAFGDEQVFTNIASFSGEITAKSASEMGGSTIFRAFGNPTELA
                     PNGSWAAGGFWGYQKVPGSAEEWRQLSAVLDEWNGNGFLVVLHLPEDLATRMPGSNAW
                     HGKIAEQFGQKRPDQYLQGGGEQVIINLDETLKTELNRIGEQVKASGQAVSIEINGVN
                     AEFYPTGWKEVEGVHGYSKPEGIEPPYVTYTRELHDDELRSKVTERTILNAARIEYQT
                     TTGSEQ"
     gene            2378..>2959
                     /locus_tag="ACHOWS_005127"
     CDS             2378..>2959
                     /locus_tag="ACHOWS_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050547.1"
                     /translation="MITIEKTKYPMSDEQVRRKLFWVLQRLSSYSLWQRKRDAWAYFT
                     QQYEQALKNWPEEITKGFDPEAIIWAYDALRLYDEGLSELAVGNRQVWQRLTGEFHQL
                     ARPVDLIESYFYLPCHERGVQQEPYPVEVEKLNKLKIAAKIHGDYLMYPPHNNVCNFF
                     DAAYLLELDNYKYNFNKLAYPVFPENLPPVPERS"
BASE COUNT          786 a          595 c          908 g          670 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa accgctcaat gaagccgtgc ttttctcggc gccggaaggc
      121 attgccctga ccagcggtga agatgtgcag ctggctgctg gtcagaacct ggctgtcaac
      181 gccggaggtg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagagg
      241 ctggggcttt ttgccaggag cggacaactg agcgtcaaat ccggggaggg gccagtagag
      301 gtacaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac gctgagttcg
      361 gcaagtgata tctcctttgc cgggaaaaaa cgtatcacgc tgattggcgg ggggagttac
      421 ctgcggctgg aggcggggaa aatagagtac ggtacgacgg tgtcgtatct gcgcaaagtt
      481 aaacggacta tagcggcggg ggctgcgtca cagcagccgg aaatgccttg gcttgccgac
      541 ccggttaaac cctctgccag tgcgctgatg cagtatctgt atcacgacga tgtcccggta
      601 gtgggcgcgc ctttcaaagc gactcttgct gatggcactg tacgggaagg taaccttgac
      661 ggtgcgggat atctgcatct ggaagacgtc ccgccaggga gagtgctggt ggagctcggg
      721 ccggatacca gagattattt acgtaaaaat aagaccgata atcggttgtt tgttgggaca
      781 tctctcagcg aggctgacat tgactcactg atagcgaagt acagcgaagg tgtggaatga
      841 gtggagtaaa cgttgcctgg tttaaggaaa ccagcctgga aaccggacag tggatctggg
      901 ggacgcttca gggggcattt aatgaaaaac agaccattgg gcagatcatc gttgatgcgg
      961 tgatcggcat gatcccgctg gtcggcgatg taacggcagt gcgagatctg atagcggttt
     1021 cgataggact ggcaaaagaa ccgaagaaac gggaaagtgt gactgaatgg gtgtttctgg
     1081 tgatcctgct gtttgcgcta atcccggtaa ttggtggcgt gataaaaggt gtcggacgtc
     1141 tggccctgcg tgtgacaaca aaagtggcag agaatgtgga aacccttggc gctgtgatta
     1201 agtttctgaa ccgtatcggc gaaggtgatg cggtgaagtg gctgaataag ctgaatgtcg
     1261 ttgagtatca ggcacagttg attgaaaaga tgggggattt ttgcaacacg atgattaccg
     1321 ccatcaataa aatcctgaaa gcccgggtcg ggcggatgtt gccggagaaa tggcaggcgg
     1381 atttactctt ggttcgggat ggttttgcgg cactgcaacg gctggcgtcg agaatgatcc
     1441 cgcaggcgtt gaaagagctg gatgcgaaac tgcgggtgtt gcaggcgctg gtttataaag
     1501 gtgaaataca tacaatcacg acggggatgg gacgtgctga agtgcgccgt gaggctgaag
     1561 catatcttat tgagcgtccg ctgtgtgaag cgccgaagca ggggacgcgg tatccgagtg
     1621 ttcatgcgga ggtaattcac gaagacagat tgcggcttaa atattccagg gagggatatc
     1681 cagatttgtt gggatataaa ggaaaaagtc cggcatttgg tgatgagcaa gtatttacaa
     1741 atattgcctc ttttagcggc gagattaccg caaaaagtgc aagtgagatg ggcggtagta
     1801 cgatttttcg cgcttttggt aatccaacag aattagcacc caacggttct tgggcggctg
     1861 gcggattctg ggggtatcaa aaagtgccgg gcagtgctga agaatggcgt cagctttcag
     1921 ctgtactgga tgagtggaat ggtaacggtt ttctggtggt gctgcatttg ccagaagacc
     1981 tggcaactcg tatgccgggt agcaatgctt ggcacgggaa aattgctgaa cagttcggac
     2041 agaagagacc tgaccagtat ctgcaaggtg gtggcgaaca ggtaattatt aatctggatg
     2101 aaacgttgaa aacagagttg aaccgcatcg gggagcaggt aaaagccagc ggtcaggctg
     2161 tcagtattga aattaatggc gtaaatgccg agttttatcc gacgggctgg aaagaggtgg
     2221 aaggcgtgca tggttacagt aagccggaag ggatagaacc gccgtatgtg acgtataccc
     2281 gtgaactgca tgacgatgaa ctgcgcagca aagtgacaga gcgaacgata cttaatgcgg
     2341 cccgcattga gtaccagacc acgacaggaa gcgaacaatg attaccattg aaaaaacaaa
     2401 atacccgatg agcgatgagc aggtacgtcg caaattattc tgggtgttac agcggctgag
     2461 cagctatagc ctttggcagc gcaaacggga tgcctgggcc tattttaccc agcagtatga
     2521 acaggcgctg aaaaactggc cggaagagat aacgaagggg tttgatcccg aggcgataat
     2581 ctgggcatat gatgctttgc gtctttacga tgaagggcta tcagaattgg cagtgggaaa
     2641 tcggcaggta tggcagcgtt taacgggaga atttcatcaa cttgctcgcc cagtggattt
     2701 aattgaatct tatttttatc tcccctgcca tgaacgaggt gtacagcaag aaccataccc
     2761 ggtagaagta gaaaagctta ataagctgaa gattgcagca aaaatacatg gtgactatct
     2821 aatgtatcca ccacataaca atgtatgcaa ctttttcgat gctgcatatt tattagaatt
     2881 agataattat aaatataatt ttaataagtt agcgtatccg gttttcccag aaaatctccc
     2941 ccctgttcca gaacgcagc
//
LOCUS       DAZKJR010000272         2939 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKJR010000272 DAZKJR010000000
VERSION     DAZKJR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.274"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1074
                     /locus_tag="ACHOWS_005128"
     CDS             <1..1074
                     /locus_tag="ACHOWS_005128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050548.1"
                     /translation="KADAEEAAEARVFYEKAFSNVYQTDDLYARTDATSLFAPAAIKL
                     AKSTAQWATILEKNAGAQMSQDQNAGGETRYIYNGISGSDVITVGKSLGGTGLNMTAT
                     RNDMKVMTGDGDDIIITGQDYGRLASAGQWDYKYLTEMGNGNDTLIVGASNSNLNVIM
                     FNDGSIAAVKKDGAQLGSVIPFDSAYDTADGGNISGTTIDMGSGNDTVLALGHENGGT
                     AIINSTIKLGAGNDTIQINGDVKGGKSPSVITGDAGMDTLIISNGSVYSEHFSGFENI
                     ELGSKGEVKIVAADLVGKDSNSIQGGMLKIIGNSDSKVDLDGSDWIKGEIKNEGDITY
                     NVYTHASAPNISVLIEDKITQVI"
     gene            1153..1962
                     /locus_tag="ACHOWS_005129"
     CDS             1153..1962
                     /locus_tag="ACHOWS_005129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050549.1"
                     /translation="MGMFNYKIYNAAHVTYLVSSSYKLAIYSNTGKVLGLGSFLASKL
                     FAKHPDKIDYSPDALNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSLGGGFTNIMARLSESLADGFYANSDYIAWASPLIFDGDGKVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFL"
     gene            2101..>2939
                     /locus_tag="ACHOWS_005130"
     CDS             2101..>2939
                     /locus_tag="ACHOWS_005130"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050550.1"
                     /translation="MDEQSGWTSWYSWVHDKGDDSRERPVFIIGNDGNNLLAGGQGGD
                     YIEGAGGNDKIEPGKGANRVDGGSGYDTLLLGDVMDDWNIYRVSDGTLFFHSERGAGL
                     TEATGIEGISFSGDWRSKLSPYQIEEEQITDHRYLLKWRNKNVDYGKHTEGTNGDDSL
                     FGNIIFGLDGNDILHSAGGEGVSLLHGGRGNDYLHGASGNDELYGAEGNDWLYGGGGN
                     DYLYGGTGHDVFVFDAACEGNSFIMDFNKHKGEKDFLFLSKDIVTSTDEFISCLKQTG
                     NDV"
BASE COUNT          851 a          461 c          769 g          858 t
ORIGIN      
        1 aaggcggatg cagaggaagc ggcagaggcc agagtctttt atgagaaagc attctcaaat
       61 gtgtatcaga ccgatgatct gtacgctaga actgacgcaa catcattatt cgcacctgcg
      121 gcaataaaat tggctaaatc cacggcccaa tgggcaacaa tccttgagaa gaatgctgga
      181 gctcagatgt ctcaagatca gaatgcaggg ggagaaacac gatacatata taacggtatt
      241 tccggaagtg atgttattac agtaggcaaa agcttgggtg gcacaggctt aaatatgaca
      301 gcgacgcgta atgacatgaa ggtaatgact ggtgatggcg atgatataat tatcactggt
      361 caggattatg gtcgtctggc aagtgctggt cagtgggatt ataaatatct cacagagatg
      421 gggaatggaa acgatactct tattgtcggg gcatcaaact ctaatctgaa tgtaattatg
      481 ttcaatgatg gtagtattgc tgcagttaaa aaagatggtg cccaattagg gagtgtaatt
      541 ccttttgatt ctgcttatga cacagctgat ggaggtaata tatcaggaac tacaattgat
      601 atgggaagtg gtaatgatac tgttttagcc ttggggcatg agaatggtgg aactgctatt
      661 attaactcaa caataaaact tggagcaggt aatgacacta tccagatcaa tggggatgtt
      721 aagggaggta aatctccttc tgtcatcact ggtgatgctg gtatggatac attgattatt
      781 agtaatggta gtgtatactc tgaacatttt tctggctttg agaatattga gttaggtagt
      841 aagggtgaag taaaaatagt agcagcagat cttgtaggaa aggatagtaa ctccattcag
      901 ggaggaatgt tgaaaatcat aggtaattca gactctaagg ttgacttgga tggaagtgac
      961 tggattaaag gtgaaatcaa gaacgagggc gatataactt acaatgttta tactcatgca
     1021 tctgcaccta atatcagcgt gttaatcgaa gataagataa ctcaggttat ttgatttgag
     1081 ctaaatagtg acaaggccgc gctgtatgcg tggcctctta atatatagat ttggtaattt
     1141 aaaggagtct gtatggggat gtttaattat aaaatttata atgctgcgca tgtaacatac
     1201 cttgtttctt cttcttataa gcttgctata tatagcaaca caggaaaggt tcttggattg
     1261 ggcagttttc tggcatctaa actatttgcg aagcatccag acaaaattga ttactctcct
     1321 gatgctctta atgaaggctg gcgtgatatt acagtggaag aactcaggtt atcttcggaa
     1381 tatgtcgatt caagaggttt ttatcgcttt cccagtccgg ttacaggaaa ccctgcgtca
     1441 ggatttggtc ctcagttgaa ggttgttggg ttgtttgatg ctcaacagaa tctacagaaa
     1501 attgcaatat cattctgtgg aacaaatgat atctgcgata ttgttgatta tacacagctc
     1561 aataatggaa aagcgctgtc tgaagcagaa aacctgcttc aaagtatcag agactttgca
     1621 atcagtaatg gtttaagtgg tgaagatgtt attgtcacag gctatagcct ggggggcggg
     1681 tttactaaca ttatggccag actcagtgag tctctggctg atggttttta tgctaattct
     1741 gactatatag cctgggcttc cccgctgata tttgacgggg atgggaaggt attaaatatc
     1801 ggttttgaaa acgatccggt atttcgtgtg ctgggagatg aatccgggtt ctgggaggca
     1861 gttaaagcaa tggggccggg cttgacaacc ccggatcgtg aatatgactc ttctcctgat
     1921 aacattgttt tatacacaga cctttatggt tcattcctct gaaatattat ccccttttct
     1981 gtgcttgctc tcccgctggg aagctggagt gcgcattttg cgggttcaaa aactgacgcc
     2041 tttgaaagaa ttttagggtc tcagttttac gactacatgt caccggacag tacagtcatt
     2101 gtggatgagc agagtggctg gacatcctgg tattcatggg ttcatgacaa gggggatgac
     2161 agtcgtgaaa gacctgtatt catcatcggt aatgatggca ataatctgct ggcaggtggg
     2221 cagggtggtg actatataga gggggcagga gggaatgata agattgaacc aggtaaggga
     2281 gcaaacaggg ttgatggtgg cagtgggtat gacacgcttc ttcttggcga tgtaatggat
     2341 gactggaata tatacagggt cagtgacggg acactgtttt tccactcaga acggggagcc
     2401 ggtcttactg aggcaacagg tattgaaggt atctcttttt ctggtgactg gaggagtaaa
     2461 ctaagccctt atcagataga agaagagcag attacagacc acaggtatct tctgaagtgg
     2521 cgtaataaaa atgttgatta cggaaaacat acagaaggta caaacggaga tgatagtctg
     2581 ttcggaaata ttatttttgg tcttgacgga aatgatatat tacatagtgc cgggggagaa
     2641 ggagtaagtc ttttacacgg aggtcgcggg aatgattatc ttcatggggc ttctggtaat
     2701 gatgagttgt acggagcaga aggtaacgac tggttatatg gtgggggagg taatgattat
     2761 ctgtatggtg gtactggtca tgatgtcttc gtctttgatg ctgcttgtga agggaattca
     2821 tttatcatgg attttaataa acataagggt gaaaaagatt ttctgttttt atcaaaggac
     2881 attgttacat cgacagatga gtttatttca tgtctgaaac aaaccggaaa tgatgttga
//
LOCUS       DAZKJR010000273         2923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKJR010000273 DAZKJR010000000
VERSION     DAZKJR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.275"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..901)
                     /locus_tag="ACHOWS_005131"
     CDS             complement(<1..901)
                     /locus_tag="ACHOWS_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050551.1"
                     /translation="MRMFTNLLYDICTVFELFKEGESPRDKRKSTDFGAHQRFWDQRY
                     NELSHIIDAEGVYSLEQRRIIFSRYEYFYYMMNSYPVYSTLKSEYIRNYFLKSFGVVF
                     IVLDIYNTYRPENETGFYYHIYNFLQKSYCPCLDYSGTESDEAAVKRYLREYLAELGF
                     NREDFRENGKMYELGKYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELD
                     RILNNIDKFYYAFYSLSILLDMQRKVKILDSIAYYLRVLIREGLWVHGLYGYAARYLY
                     DFNIFDTTPYARALLERFHEFESG"
     gene            1002..1250
                     /locus_tag="ACHOWS_005132"
     CDS             1002..1250
                     /locus_tag="ACHOWS_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0050552.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            1266..2831
                     /locus_tag="ACHOWS_005133"
     CDS             1266..2831
                     /locus_tag="ACHOWS_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFQ0050553.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSIMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACTKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRICMTLSNAASHDLEIFHKEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDKWLRENIIRVKGYEYQKSHILQYGPYALRNKCVLDEAL
                     DILAEQKKIVIDYSIGQKIIYIGDAITPCELANEANIPIMERGMFMVCWDHKLNKYRN
                     EEQTKIWNITTKN"
BASE COUNT          933 a          657 c          553 g          780 t
ORIGIN      
        1 ggccactctc aaactcatga aaccgttcaa gtaatgctct tgcataaggc gttgtgtcaa
       61 aaatattaaa atcataaaga tagcgtgcag cgtaaccata caacccatgt acccaaagcc
      121 cttctcttat cagcactctg agataatatg caatactgtc aagtatcttt accttccgtt
      181 gcatatcaag caaaatactt aatgaataaa atgcataata gaacttgtca atgttgttga
      241 gtattctgtc cagctcactc ttatcaagct tcttctctct gtaatccttc ttgtattcat
      301 tcttacaggc ctttatatat tgtttcataa ggctcttcct tttaccgtac ccttttcgaa
      361 tagttccctg gtattttccc agctcataca tttttccgtt ttcccgaaaa tcttctctgt
      421 taaatcccag ttcagcaaga tactcccgga gataacgttt aacggcagct tcatcgcttt
      481 ctgtaccaga gtaatccagg cagggacaat atgatttttg caaaaaatta tagatatgat
      541 aataaaaccc tgtttcattc tccggacgat atgtgttata tatatccagc acaataaaaa
      601 caactccaaa gcttttcaga aaatagttac ggatatattc ggactttaat gttgaataca
      661 cagggtaaga attcatcatg taataaaaat attcataccg ggaaaatata attcgccgtt
      721 gttccagact atacacccct tcagcatcaa taatgtgact gagctcatta taacgttgat
      781 cccagaatct ttgatgcgct ccaaaatcag tactcttccg cttatctctc ggactctctc
      841 cttccttaaa caactcaaaa actgtacaaa tatcatacaa aagatttgta aacatgcgca
      901 tctgctctga tctccggttg gtttccgttt tatcacaggg gacattctgg ccttaaccta
      961 gcaaattaat gacatagcct tcaactcaca tggagaacaa tatgtcaacc cagaccggac
     1021 atttttatac cccctcacgc atcctgcgta tggctgaact tacccggctc cttggtatat
     1081 caagatcgac catctacgaa aaaatgaatc cggcatcaaa atactacgat gccacctttc
     1141 cccggcctgt ccggcttggg agttcggctg taggctggcg ggcatctgcc attgatgagt
     1201 ggttaacttt acatacaaca ccaaccagat ccgtaactaa ggataaataa ggaaaactgt
     1261 acgccatgac aaaattatcc ccccctgaac ttgacataac taaactgcct gacaggtgcc
     1321 aggcattact tgatgaaatg catgaagaaa cgggaataag ccgtgaaata ctgctgtcta
     1381 ttatgctgac ggtcaaggct gcctcggtcc aggatacgca tgaagttgaa ctttctggag
     1441 ggcagcgtac cagtcttcag atatatatgt gtctttcatc agcatcaggc agtggaaaaa
     1501 cctctgcctg cacaaaatta atcgcccctg ttcatgaaac agaagaagaa ctacatcagg
     1561 catatatcga cgataaaaaa aactatgatc gcatgatgga aatgtggaca acagataaaa
     1621 aaatcctgga acggagatat aaaaaagaaa tggaaagatc cccggaaaat gctgctgctg
     1681 cacgagcagc gcttgaaaag tgtatagcaa ataaacccgt acctcccgta caacaggttc
     1741 tcatcgtgaa tgacgcgaca ccagaaggga tagctttgaa actcagccag tcaccttctc
     1801 ttctgttgct gtctgatgaa ggaggaacta ttctggataa gcgtttcgaa cgcaaatcgg
     1861 cgctgtacaa cacattatgg agtgggcaac cagtcaccgt agaacgggca tccagacccg
     1921 ggttccgggt aaaggattcc cgtctcacta tgcttatact cactcaaccg gtaatatttg
     1981 ataagttttt tactctcact ggcgaccaga tccggggcaa tggctttctg gcccgggtgc
     2041 tattttgcga acccggagca aataaaataa tgacgacaga accgcatact gccactcctg
     2101 ttgtaacgca gcaatgtgca acctgttttc atgagaaaag ttttggcagt cagatcagag
     2161 actctctgag agcgtcccgg gaaaggcgtg caaaaggtga gcaacgcatc tgcatgacat
     2221 tatcaaacgc agcatcccac gatctggaaa tctttcacaa agaaaatatg agtgcagtca
     2281 ggcagaaccc acgtatgacc actttcgaag acattatcgt caggaaaaga gaacaggctg
     2341 ttcgaatagc cgcacttctg gagctggaga acaatccaca tggtacagta ataacacgag
     2401 aaagtatcaa tagtgctatt tatctcgttg atttttattt tcagcatctt atatccaaac
     2461 tggaatcact tcgggaaata tctcctgcag aaaaactgga taaatggctc agagaaaata
     2521 tcatccgggt aaaaggatac gaatatcaaa aaagccatat actacaatat ggtccatatg
     2581 cgctcaggaa taaatgtgtt cttgatgaag cacttgatat acttgcagaa cagaaaaaaa
     2641 tcgtaattga ttattcaatc ggccaaaaaa tcatttatat cggggacgcg attacacctt
     2701 gtgaattagc aaatgaggca aacatcccga taatggagcg tggaatgttt atggtctgtt
     2761 gggaccataa gctgaataag tacagaaatg aagaacagac taaaatatgg aacataacta
     2821 caaaaaatta aagttcccgt cttttatcat tcagttacca acctgaatat ctgaaacaat
     2881 gccagacatc acttacagac tggcattgtt tttatcattc aat
//
LOCUS       DAZKJR010000274         2885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKJR010000274 DAZKJR010000000
VERSION     DAZKJR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.276"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(207..>2885)
                     /locus_tag="ACHOWS_005134"
     CDS             complement(207..>2885)
                     /locus_tag="ACHOWS_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFQ0050554.1"
                     /translation="DKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVM
                     KERYVQPLAKLLANYKISQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAA
                     EIMQRVRNSGKQAQYDRLAGIVDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLK
                     GQDVDGVVSLPRTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVG
                     NAFLKLVQDNPDKDYWQVFTDDRPDTMRTIAERKDQETGETRREVVERPVPMAMMADR
                     YFTTKKNGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFL
                     VSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLT
                     GKGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFL
                     NLVEDANGAVENALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYM
                     FANASIQGTANLVRTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSV
                     AGEDDDGVNWYDKVPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEG
                     VAAGDLTASRAAGNVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNEN
                     FMGAQIYQENMPFGTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDVTPES
                     LKYWIDYISGGTGRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYD
                     RMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADS
                     TLSLAQQSAMVKSVERDMKIAVDRFNREYNKKVGVE"
BASE COUNT          602 a          874 c          666 g          743 t
ORIGIN      
        1 ggtgtagtca ctcagaacca cgactacacc accggaacag gcatcagaaa aaacttactc
       61 tcatcaggca ttctgtttca catctgctta cactgtaatt tctgatcaat atcacaacat
      121 tatccgaatt taactgttca gttcatctac aatatttccc tgcgtgactt tatatcagcc
      181 ccgcacttcc gtacggagca tgttctttac tccactccca cttttttgtt gtactcgcgg
      241 ttaaaccgat ccacggcaat tttcatatcc cgctctaccg atttcaccat cgccgactgt
      301 tgcgccagac tgagagtact gtcggcgtaa atggcatcac gctgtttacg caaatccttc
      361 agtctcttcc gggtatcctg cataagcccg ttcatcgaca attttccgtt gttctcgtca
      421 atgaacgccg ttctttctgc accggtcaga ctcttcagct ctgcgtggta ctgcgcaacc
      481 tctgtcatcc ggtcgtacat cttctgctgg tcagcatacg gcatcacctc acctgaaatt
      541 ttccccagga aaggcacctg ctgctccggt atatcaatac cattcagcga tttcaccgcc
      601 gcatccgtgg ttttggaaat gaagcgccct gtaccaccag agatatagtc aatccagtat
      661 ttcagtgatt ccggcgtgac atccaccgcg ccgggacggt actggctgcc acctgagaac
      721 gcattcagcc aggatgcaaa cgccttgtac gcttctggcg ttgaacgtct tcccagctgg
      781 ctgtcaggtt ttggtgtacc aaacggcatg ttctcctggt aaatctgcgc ccccatgaag
      841 ttttcattca tggcaaggtt cgcaaacgga cgcagaatgg tcggcgctgc atttttcagc
      901 aatgccccgg acagtgtttc cgacgtctca ctgccaatcg ggctgaatgc accaagcaca
      961 ccaccaacaa cattaccggc agcacgggac gccgtcaggt cacccgccgc cacaccttca
     1021 gcggtatgcc cgagcaggaa gaaaacgttg tacccgtaag gcagaggaat actccagtac
     1081 tctccggcct tgcccccgaa caccgatttc ataatgacga ggttacgctc tttcacatga
     1141 gacggcacct tgtcatacca gttaaccccg tcatcatcct cacccgccac actgcggtta
     1201 agcgagccaa gcagataacc tgctcccaca gcggcaagcg cgattttctg cggaacattc
     1261 agattcttcc agcgaaggcg ctccagtaaa ggtccctcac cattaagatg tccgagcgtt
     1321 ctcaccagat ttgccgttcc ctgaatactg gcgttggcga acatgtacag cgagttcatc
     1381 agcgccccct gctcaccacg acggttaaag ttcaccgtca tgtttttggc aagagacgcc
     1441 gcctgctggc gtgacaaacc ggcatcacgg gcgtgtttat aagctgacag acgcagtgcg
     1501 ttttcaaccg cgccgttggc atcctcgaca aggttaagga acgaattcca tgcaccgata
     1561 ctctggcctt tccatcctcc cttcgccagc gaaacaaggc gatccatttc cttctgctgg
     1621 ccttcaaggt cacccatgtt aaaccaaccg gttttacctc cgtcctcaac aaactctttc
     1681 cacaccttct gccactgtgc acctttgccc gtgagggttt taccacgcag actggcgtat
     1741 acggctgaca tggcagaacg gctgtctttg actacagcca gggcggataa gttatccagc
     1801 cctttcagtt taccgtcgct ccttccctgc tcagccttca ggttcatcac cgccgtctgc
     1861 acgtcacgga tgaagttact gaccaggaat tccgggttat acgacgtgtt caccgttgcc
     1921 aggaagcggt taactttccc cagcgtacgg attacggcgt tactggtttc cggtcccata
     1981 ttcttcatcg cacgcatcag gcgcgggtca tggagtttga tgtagtacgt tttgccgttc
     2041 tttttggtgg tgaagtagcg gtctgccatc attgccatcg gtacagggcg ttcgacaact
     2101 tcgcgacggg tttcaccagt ttcctggtcc ttgcgctctg caatcgtccg catggtatcc
     2161 ggtctgtcat cggtgaatac ctgccagtaa tccttgtcgg gattatcctg caccagtttc
     2221 aggaaggcgt tacccacttc gtttttgcga tggcggatca gcgattcgct caggtcctgt
     2281 atcgcctgag tggacggaga ctgtgcacgg gatgcacgcc ccatggcctg cctgctttca
     2341 cgtccgccga tggtgaagcc cttacctgta cggggaagtg acaccacacc gtcaacatcc
     2401 tgacctttca ggggaacgta gtaacggtag gcgttctgcc aggcatccac cacaccgttc
     2461 tcttcaagtc cggcctcacg gataagctca cggcgacggg ccagcatatc gtcaacaatc
     2521 cctgccagac ggtcatactg tgcctgtttg ccgctgttac gcacacgctg catgatttcc
     2581 gccgcctccg cgttggtcat ccccgaaccg ccgtccggca ttttagggtt gattttcgca
     2641 atatgtgcgt tacgttccgg cgcgtgacgg gcgtagaggt actcatccag atcggcctga
     2701 gagattttgt agttcgccag caatttggcc agcggctgaa cgtagcgctc cttcattacg
     2761 ttcaggtcgt tttccgcctt cccgtggaag agttcttcag ccatataggc gttattgctg
     2821 tcatctattt ttccgccagt tttacggata ttctcctgca ccgccttcag cacctggaat
     2881 ttatc
//
LOCUS       DAZKJR010000275         2821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKJR010000275 DAZKJR010000000
VERSION     DAZKJR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.277"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..2426
                     /gene="ydeP"
                     /locus_tag="ACHOWS_005135"
     CDS             147..2426
                     /gene="ydeP"
                     /locus_tag="ACHOWS_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFQ0050555.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTIGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2674..>2821
                     /gene="safA"
                     /locus_tag="ACHOWS_005136"
     CDS             2674..>2821
                     /gene="safA"
                     /locus_tag="ACHOWS_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFQ0050556.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMG"
BASE COUNT          764 a          657 c          679 g          721 t
ORIGIN      
        1 atcttttgtt taacgataat agattaaaat agaaagagag tagtccccta ctcttatata
       61 tccatgttga cgataatccc tttgtatata ctgtgcatca tcgctattac aaatcctaat
      121 aattcatttc cacacaggat aagcagatga agaaaaaaat tgaatcctac cagggagctg
      181 ccggtggttg gggtgctgtt aaatccgtag caaatgcagt acgtaagcag atggatatac
      241 gccaggatgt tattgccatg tttgatatga ataagccaga gggctttgac tgtccgggat
      301 gtgcatggcc agatcctaag cacagtgcgt cattcgacat ttgtgaaaac ggcgcaaaag
      361 caatcgcctg ggaagtcacg gataagcagg taaatgcctc tttctttgct gagaatacgg
      421 ttcaatcatt acttacctgg ggagatcacg agcttgaggc tgcgggacgt ctcactcagc
      481 ctttgaaata tgatgacgtc agcgactgtt acaagccatt aagctggcaa caagctttcg
      541 acgaaattgg cgaacgcctt caaagctata gtgatcccaa tcaggttgaa ttctatactt
      601 cgggccgcac ttccaatgaa gctgcctttc tttatcagct ttttgcccgt gaatacggga
      661 gcaataactt ccccgactgc tccaacatgt gccatgaacc gaccagtgtg ggtttggcag
      721 cgagtatcgg tgtaggtaaa gggaccgtgt tgctggaaga ctttgagaaa tgcgatttag
      781 tcatttgcat tgggcataac cctggtacaa accaccctcg catgttgact tcgttgcgcg
      841 ctttagtgaa acggggagcg aaaatgatcg ccatcaatcc tctacaggaa cgtggcctgg
      901 agcgatttac cgcaccgcaa aacccgtttg aaatgctgac gaactctgag actcagttgg
      961 ccagtgccta ctacaacgtg cgcattggtg gtgatatggc gttgctcaag gggatgatgc
     1021 gcctgttaat tgagcgcgat gatgctgcaa gcgccgcagg tcggccctca ttgctggatg
     1081 acgaatttat tcaaacgcat accatcggct ttgacgagct acgccgtgac gtgctcaatt
     1141 ccgagtggaa agatatcgaa cgtatttctg gactaagtca gacacaaatc gccgaacttg
     1201 ctgatgcata tgccgctgcc gaacgaacca ttatctgtta cggaatgggg atcactcagc
     1261 acgaacatgg tacccagaac gtacagcaac tggtcaatct gctgttgatg aaaggtaaca
     1321 ttggtaagcc tggtgcgggt atctgcccac tacgaggtca ctctaatgta cagggcgacc
     1381 gaaccgtcgg tatcaccgag aaaccgtctg cagagtttct ggctcgcctg ggtgagcgct
     1441 atggtttcac cccacctcat gcacctggac atgctgcaat tgccagcatg caagcaatat
     1501 gtacggggca ggctcgagca ttgatctgca tggggggcaa ctttgcactg gcaatgccag
     1561 atcgggaagc aagcgctgta ccgttaacac aattagatct gacggtacac gtagccacta
     1621 agcttaaccg ctctcatctg ctgaccgcac ggcatagcta tattctgccg gttctgggac
     1681 gcagcgagat tgacatgcaa aaaagcggtg cgcaggcggt aaccgttgag gattcaatgt
     1741 cgatgattca tgcctcgcgt ggcgtgttaa aacccgccgg tgtaatgctg aaatcagagt
     1801 gtgcagtggt cgcgggaatc gcgcaggcag cactacccca gagcgtggta gcctgggagt
     1861 atctggtgga agattatgat cgcattcgca atgacattga agctgtgctg ccagagttcg
     1921 ccgactataa ccagcgcatc cgtcatcccg gtggttttca cctgataaat gcagctgctg
     1981 aaaggcgctg gatgacgccg tcaggtaagg ctaatttcat taccagtaaa gggctgttag
     2041 aagatccctc ttcagcgttt aacagtaaac tggtcatggc gacagtacgc agccacgatc
     2101 agtacaacac gacgatttat ggtatggatg atcgctatcg aggtgtattc ggtcaacgag
     2161 atgtggtctt tatgagtgct aaacaagcta aaatttgccg tgtaaaaaac ggcgaaagag
     2221 ttaatcttat tgcgctcacg ccagacggta aacgtagctc acgccgcatg gatagattaa
     2281 aagtggtcat ttaccctatg gctgaccgct cactggtgac ctattttcca gaatcgaatc
     2341 acatgctaac acttgataac cacgatccat taagtggcat tcctggctat aaaagtattc
     2401 cggttgaatt agaaccatca aattaattcg cctcctcatt tcttctgctg tcatccgcac
     2461 agcagaagaa ttcctcattg accattgtcg cgcaatttac tcacatgtgc taaattaaac
     2521 tgctcgttat aagatatata cctccgccta cagctgtaag aaactccgct cagtactgaa
     2581 gcgccagtcc tatttcccct attctccagc ctgttatatt aagcatactg attaacgatt
     2641 tttaacgtta tccgctaaat aaacatattt gaaatgcatg cgaccacagt gaaaaacaaa
     2701 atcacgcaaa gagacaacta taaagaaatc atgtctgtaa ttgtggttgt cttattactg
     2761 acacttacgt tgatagccat tttttcggca attgatcagc tgggtatttc agaaatgggt
     2821 c
//
LOCUS       DAZKJR010000276         2817 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKJR010000276 DAZKJR010000000
VERSION     DAZKJR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.278"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..589
                     /locus_tag="ACHOWS_005137"
     CDS             <1..589
                     /locus_tag="ACHOWS_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFQ0050557.1"
                     /translation="VDKNIAEAVVSDEGAAGGKALQLNKPESHIWFLEHDAAGQGAEL
                     LKKGGRVSVRFKVPGALVPNQFALGIYWQLSSLPEGVTLAEEGNDMLMSFFLQTDATN
                     LNAMYHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPAS
                     GAADKLQLTDISKAATYTLLIDSVKVEVNNADAAA"
     gene            728..910
                     /locus_tag="ACHOWS_005138"
     CDS             728..910
                     /locus_tag="ACHOWS_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFQ0050558.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            936..1181
                     /locus_tag="ACHOWS_005139"
     CDS             936..1181
                     /locus_tag="ACHOWS_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFQ0050559.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGDPTPDSDMMM"
     gene            1258..1473
                     /locus_tag="ACHOWS_005140"
     CDS             1258..1473
                     /locus_tag="ACHOWS_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFQ0050560.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAERGE"
     gene            1477..1725
                     /locus_tag="ACHOWS_005141"
                     /pseudo
     CDS             1477..1725
                     /locus_tag="ACHOWS_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
BASE COUNT          763 a          598 c          795 g          661 t
ORIGIN      
        1 tgttgataaa aacatcgctg aggctgtggt cagtgatgag ggtgccgccg gtggtaaggc
       61 cctgcaactg aacaaaccgg aaagccacat ctggtttctg gagcatgatg ccgccgggca
      121 gggggcagag ttgctgaaga aaggcggacg tgtgagcgta cggtttaagg tgccgggcgc
      181 actggtgccg aatcagtttg ccctgggtat ttactggcag ttgtcgtccc tgccggaggg
      241 agtgacgctg gcagaggaag gcaacgacat gctgatgtcc ttcttcctgc agacggatgc
      301 gacgaacctg aacgcgatgt accacaagaa gcagaatgcg aagctggata cgttcggggt
      361 ctttgataac ggatggcaca cactggcttt tgagtttgcc ggaaacaaca gcattcaggt
      421 gacgccggta ctggatgaga aacggggtac accgttcaca ctggtgaaat ctccggcatc
      481 aggggcggcg gacaaactgc aactgacaga catatcaaag gcggcgacat atacgttgct
      541 gattgacagt gtgaaggtgg aagtgaacaa cgcggacgcc gcggcatgat aaaaagagcc
      601 gccagcggca ggaatggaag ctggcggagg taatcccaat ggagaatgta aagaaaagat
      661 gctttcgtac atcggttgtt ttttaaatga aaacagttct cattgtcaac cataacggta
      721 agaaattatg acatttattc atcaggtgat gctgtacttc tgtacggcgg tctgtgtgct
      781 gtatcttctt tcgggtgggt acagggcagt gcgcgatttc tggcgcaggc agattgataa
      841 aagggccgca gagaaaatca gcgccagtca gtcagccgga agcaaacccg aagagcccgt
      901 tactccttaa caaccccttt cagcgagaaa atcccatgtc agaaatcaca tccctggtca
      961 ctgcagaggc agtgaaggaa gtcctgcgct ctgaagaagt cctgagcgca ctgaaacaga
     1021 aactccgcca gaaccttgag tcgcgtcttg atgcagaagt ggatgccatt ctggatgaac
     1081 tgctgggcgc accggcagtt ccggagccgg aaggtatcgc gggtgagggg agtgcttcgg
     1141 atggcggtga ccccacacct gacagcgaca tgatgatgta agcatgcgca agggaccatc
     1201 ggtgtgtgcc ggtggtcttt atattgttgt gagcttccgg attgcgggag acggggtatg
     1261 taccagatgg aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt gggaacgggc
     1321 tactggttcc tgcagttgct ggacagggtt tccccgtctc agtgggcggc aataggcgtg
     1381 ctggggagtc tgctgtttgg tctgctgaca tatctgacga acctgtattt caaaatcaga
     1441 gaggaccggc gtaaggcgga gcggggagag tagacgatga ataaacaata cgaactggtt
     1501 gttaaaggga taaataatta cccggataag attactgtta ctgtggcgct ggaaactggt
     1561 gggtatacgt cactgttgtt gccaaatgtg gtgattgatc ttgatcgtgt tgaaggtgcc
     1621 ccgctggagt tttacgaagc tgaggcgaaa aagaaggcga agcagttttt catggatatt
     1681 gctgccgggt tatgtgaagg gaacgaaccg ttgccggaaa agcgcctctg ttcagaagag
     1741 cggtatacca tacaaattaa caatgcatat aacacgatac agtcagaaaa agatgatatc
     1801 gaatcacgca ttgaaaaact ggagaacagc gtcgttgaac tgaataaaaa actgtcggtg
     1861 cagatccctt ccgaaggtga aaaaaaacgc cgtgatgagc agttcgcggc gttttacgat
     1921 tattgccgga aagtgatgag cagaaatctc gcagagtgtt tccgtattca aaaaaacaga
     1981 gagcgctctg caaatagtga gattctggat aattttttaa aggggacgca tctcgccagt
     2041 gattgtttca ttgctcattc ttttggtggc tttcatgagg ccatcaaaca tgatgttgag
     2101 agaagtccag gtatcctctg ttccattccc gtaagcatcg aaatagacac aaatacgata
     2161 accggtgaac tcgttaccgc cggagtcggt atattccgtc atgaggggaa cgatgccaaa
     2221 ggttccttta caaaaatcgg gccgttcttt ctgaaggtag agcgatagtt tgttaagtac
     2281 ccatccgaca gctcgctcat tttggtttat tttcgtatcg cgaaacaaaa attcgaagcg
     2341 gccagatgac gcatgggtgc aaccaaatac cggggcgtat tcatcaacct ttggtgttcc
     2401 tgaaaacatg cagtccgtgc tctcgaatgc agatgaattg ctgttgatcc acttaacaaa
     2461 agaaaaaaag gtctgaatcg cttgtctgtc tgaatattca acaaaatcct caaggtgtgt
     2521 ctcgatcagt tccgggtgtt tacgaaagtc ataatattga ccgggacgat atttatgagt
     2581 ggttgtttta tcccatggtt gtgctctgcg tccctgtaaa cggaggtctg ccgctggata
     2641 gataagcata agtaatctcc tggtggaatg taatccacga tcatccgggg aaattaagaa
     2701 cccgccagtg cccaccactg gtggggtgaa ggcttaacat atccagggat tcggaaccga
     2761 taaatcctga taaatatcca tgaacaccaa aatcaaatac ggcctgtcgg ctgccgt
//
LOCUS       DAZKJR010000277         2802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKJR010000277 DAZKJR010000000
VERSION     DAZKJR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.279"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(737..2674)
                     /gene="iolC"
                     /locus_tag="ACHOWS_005142"
     CDS             complement(737..2674)
                     /gene="iolC"
                     /locus_tag="ACHOWS_005142"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFQ0050561.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVLDIDYRPVLWGLTSLGDGETRFIASPQVTEQLQQVLHHFDLIVG
                     TEEEFHIAGGSTDTLTALRRVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGV
                     RVDILNVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQEL
                     DDYLSREHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEF
                     TIPELKMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGS
                     YPLKLEHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHD
                     LLLEVILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDP
                     ECRGILILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLI
                     AAVKQKYRTLIDYWREYRPAL"
BASE COUNT          732 a          686 c          709 g          675 t
ORIGIN      
        1 ttaaagtatg ttgtagagaa aagtttgtct gaagtggcct actgaaatga attgcgcagg
       61 aaagtgagac ggaagatcgg tcagaccgac accagcaaaa tatgatatat ccgacgacca
      121 tcaaaggtca taaaggcgat aaggaatagg tgcgcagata tccgtcatgg ctctgcaaaa
      181 aactgtaaaa tgaagccgaa tgtacggggg cagtctgaac cccaactgaa gcatcttttt
      241 tgcacaaaat cgggagtcca tgtacggata taaagctgca acctaccgtc aatgtcagaa
      301 caatggaagc tgtgaaaaac gggatgcgtt aatataaact ccatgacagt agtggaacgg
      361 gttaatcaag caggattacc agcaagatga gggagctgat tcagcacagg cgacctgacc
      421 ctggccacgg ataaccgtct gtcgcccgtc tgaggtgtcg ttttttcgtt ctgccacccc
      481 tcggttgagt aaaatggaaa ttaaccgtca agctgcctgt agcgtaccga tcagaagatt
      541 gctcagatgg tacagctgtt gtacgttctg cggcaacgtg acgagggaac taccaatagc
      601 cagacagttc agaaatatca ttaatgcaga tacgaaacag cactgtcggg atgatgagga
      661 aagtaggacg gtcatgaaac atctttgaat acgccccgag caagaataca gacgcgggac
      721 gtaatgcaga cggatactaa agcgccggac gatattcgcg ccagtagtcg atcaatgtgc
      781 ggtatttttg tttcactgct gcgatgagct gttcatcgtt aatctggccg ctgaaccact
      841 gccgtgatgg ctcgccaaag atggtgcgac ctacggcgaa gcctttaacc catggtatat
      901 ccgcagcggc ggcaaagcca gatttcagct cgctttccgg tgcatccagt ccaagtatta
      961 aaataccgcg gcattcggga tcgtagcgct caatatgctg gctgatttct tgccaacgag
     1021 ccggacttaa cggtggaagc ttccaccagt caggctggat ccccagcagg taaaaacgtt
     1081 ccacaatctg tgtatagtaa tgctcgcttt gatctgctgc gtcgcgtggc aagatgacct
     1141 ccagcagcag atcgtgtcca gactggcagc aggcccggta tatctcatca atgagtgctt
     1201 cttgcttaag gcggagggat tctgcatcca gtgggtgata gaagacaagg catttcacga
     1261 cgtgctcttt cggccaactg acaagttgtg aaccgatatt accgtgctcc agcttcagag
     1321 gatatgaccc cggcatttcg ataggacgtc caatccacca tccctgacca gtcacgtcgt
     1381 taagtgcttt ctgaccaaaa gtagtatccg ccaggatgcc gcagttatgc agtaatccag
     1441 cctcgtgcgc cgcctgacgt gcgccttcca gtagtagcat cttcagctca gggatagtga
     1501 actcgctggc acctgtcgct ctggcgatat ccaccagttg attgcgatgg tcaaaggcga
     1561 atacacacag ttccggccag tgctgttttc gggttgtcgt tcggtgaaga tggttaaggt
     1621 gagggtcttt gtcgggtctg gtgatagcgt gctcacggga gagatagtca tccagttcct
     1681 gtttggttgg catcgcgggg gcgcagccgt ggcgcgatac aactaatgcg ccacatgcgt
     1741 tagcataacg acaagcttgc tcccaacctt catcgttcag atagcctcga agcagtcccg
     1801 acataaaggc gtctcctgca cccagcacat ttaggatgtc cacacgtaca ccgctgtgca
     1861 tctgcacctg agaccagtcg tcggcgatgt ttccttcaaa tactgaacac cctaatgcgc
     1921 cgcatttgca taccaatacc gcagaggtta gctgacgcac acggcgcagg gcggtcaacg
     1981 tatcagtact gccgccggcg atatgaaatt cctcttcggt gccaactatc agatcaaaat
     2041 ggtgcaatac ctgttgaagc tgttcagtca cctgtggtga agcgatgaaa cgtgtttcac
     2101 catcccccag cgatgtcaac ccccatagta ccggacgata atcaatatcc agcactctac
     2161 gcagaccgtg tttttgagca taggtcagcg ctttaagcac tgcggcgcgg gtattgatgt
     2221 gagaaagatg agtgccggtg acggccaggg cacgtgaaga ggcaatgaat gtttcgtgga
     2281 tatcctcagg cgccagcgcc atatccgcac agttatctcg ataaaagatc agcgggaagg
     2341 tgtcctgatc tttaatacca aggatcacca gggctgtcag acgttcagga tcagtcaaca
     2401 ggcattgtgt attgacgcca acacggttta attcttcgcg gatgaaacgt cccatatgtt
     2461 catcgcccac acgtgccagc atggcggaac gtagtccctg aattgctgtg ccgtaagcga
     2521 cgttgcctga tgagcctcct aaatattttg caaaggttgt catgtcttcc agacgtgacc
     2581 cgatttgttg accatataaa tcaacggcga cacgccccag acagataatg tccagcgttt
     2641 tttgctcact gctgactgtg ttgttcatag agatatcctc ctacagaaga ccgtgtgatt
     2701 tttgtttaca ctaaggttat ataatttcga tttcaacaaa aaggaaaaaa taattccatt
     2761 attttttgac gataatcatg cttttagatt ctatgttaca ac
//
LOCUS       DAZKJR010000278         2791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKJR010000278 DAZKJR010000000
VERSION     DAZKJR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.280"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1092
                     /locus_tag="ACHOWS_005143"
     CDS             <1..1092
                     /locus_tag="ACHOWS_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFQ0050562.1"
                     /translation="FANSKLRMEDRRGEEHIKLATEYGKTQLNSGHLVDSQGQLRGKG
                     TELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLNQQLQQLENAAERA
                     QALKADIDSQIQMFAQRLKPLNEAVLFSAPEGIALTSGEDMQMSAARNVAVNAGGDIS
                     TGTMGNLTALAGERLGLFARTGQLSVKSGEGPVEVQAQNGSMRLFAEKKLTISSESDI
                     SFTGKKRITLIGGGSYLKLEAGKVEYGTMATCMRRVKRTMTAAAQSMPMAFPRMSSSH
                     IYSAIYRLLDKHGNILVNTPCRLTTPSGQTVTGYSDNEGRSVPVYTRQQEDVELHFFV
                     KKPQPEETMWFVGETDTQQLETELRESLP"
     gene            1092..2381
                     /locus_tag="ACHOWS_005144"
     CDS             1092..2381
                     /locus_tag="ACHOWS_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0050563.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGGIGHEDRMTILEIHDCRP
                     EEHQWVDQVINVTMKAWQTSGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKAVS
                     DLSEGVVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVK
                     SWTNTQIKAAWTEIQHATDMTLDMLKQVDWVQVLTSVAKTVGVILVAVGVGGLVVTLG
                     IPEAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVG"
     gene            2394..>2791
                     /locus_tag="ACHOWS_005145"
     CDS             2394..>2791
                     /locus_tag="ACHOWS_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI immunity family protein"
                     /protein_id="HFQ0050564.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNPGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSV"
BASE COUNT          715 a          668 c          832 g          576 t
ORIGIN      
        1 ttcgctaact caaaactgcg gatggaggac cggcgcggcg aggagcatat caaactcgcc
       61 acggagtacg gtaagaccca gcttaacagc ggacatctgg tggacagcca ggggcaactg
      121 cggggtaaag gcacagagct gcgtaccgat gagtggggaa ccctgagagc ggggaaggga
      181 ctttttgttg ctgcggatgc gcaggcgaaa gcacagggcg aggcactgga catggatgcc
      241 gcgctgaagg aaatcgacag gcttaatcag caactgcagc aactggaaaa tgccgcagaa
      301 cgggcacagg cgctgaaggc tgatattgac agtcagatac agatgtttgc gcagcggctg
      361 aaaccgctca atgaagccgt gcttttctcg gctccggaag gcattgccct gaccagcggt
      421 gaagatatgc agatgagtgc agcacgaaat gtggctgtca acgccggagg tgatatcagt
      481 accggaacaa tgggcaacct gaccgcgctg gccggagaaa ggctggggct ttttgccagg
      541 accggacaac tgagcgtcaa atccggggag gggccagtag aggtacaggc gcaaaacggc
      601 agcatgcggt tgtttgctga gaagaagctg accatttcgt cagaaagtga tatctcgttt
      661 accggcaaga aacgcattac gctgattggc ggtggcagtt acctgaaact ggaagccggg
      721 aaggttgaat atggcacgat ggcaacctgt atgcgtcggg tgaagcggac gatgacggcg
      781 gctgcgcaga gtatgccgat ggcattcccc cgtatgagtt cttcgcatat ctactcggct
      841 atctataggt tgctggacaa acacggcaat attctggtca ataccccctg tcgcctcacc
      901 actccatcag gtcagacagt aacggggtat agcgataacg aaggccgctc cgtgccggtt
      961 tacacccgcc agcaggagga tgtggaactg catttttttg ttaaaaaacc acaacccgaa
     1021 gaaacgatgt ggtttgtggg ggaaaccgat acgcagcaac tggaaaccga actcagggag
     1081 agcctgccat aatggaaatg cccgatttac ccgagacacc gacatctgat attcccaccg
     1141 gggcgaacaa ccagccgtgg gactgcacta cgacgggtgt cgaaatggtc gaaaagcagt
     1201 cgcgtctgcc aaccacggaa aaccgtcagt acctgcatat aaaggtggaa tacgccatgc
     1261 gttttccgca actgtcggca caaatccgcc ggaaaggtaa aacttaccag atgagcctta
     1321 agcagaccgt tatgagtggt cttattcgtg tggatgaaat cgcccgcaat tactgctggt
     1381 attataaggc ggaggtggcg tttgatatgc gcaccacgcc accacgccct tttctgagca
     1441 gtacgctgct ggaaaaagac ggtgctggcc gtcgccacac gaccaatcct ttccctgtgc
     1501 ccggcagggg catttaccgc cgcccggacg tgattattgt taaaaataag gaggaccgct
     1561 ggcccggact ggcggggccg gataccgaag gcaatatgca cagcgataac ctggcccgcc
     1621 tggtggaggt gaagtttccg ggagataggc tgtatgaaga ccaatatgaa gagtatatgc
     1681 agattgtcgg tggaattggt catgaggatc gcatgaccat tctggagatc cacgactgtc
     1741 gcccggaaga gcaccagtgg gttgatcagg tgatcaacgt gacgatgaaa gcctggcaga
     1801 ccagcggcgc gaaatactgg ccgctggctc tctacccgcc ggtctttatg ccccgtccgc
     1861 catcaacccc cgttgctgcc agaatagagc cctggactta cgccggtaaa gcagtatctg
     1921 atctcagtga gggtgtggta cgtggtgtgg ctgaaggctg gaatgccctg tcaaaagaga
     1981 cgcaactggt cctgaattca gcagccagct ggcttaacga cagcggtgag tgggtttacc
     2041 ggcagggaag tgaggtctgg gtctgggcca gccaaaccgg taagtcggtg aaatcctgga
     2101 ccaacaccca gattaaggcc gcatggacag aaatacagca cgccaccgat atgacgcttg
     2161 atatgctgaa acaggtggac tgggtacagg ttttaaccag tgtcgcgaaa acggtcggcg
     2221 ttattctggt tgctgtgggt gtcggcggac tggtcgtaac gctgggtatc ccggaagcca
     2281 ttattggcgc gtttttactg attgttcgtc tggcaataag cgcctgggga acactggcga
     2341 cgattctcgg tacgggtacg gcggtactgg cagttggtta aacaggagca ggtatggaat
     2401 tgcatgattt tgagctgaac tacattgaga aatggctgcc ggaagcctcc gtgaaataca
     2461 aagacggcag tccggcagtc aatcccggtc tgattatcac cgtctttttt aaggacggac
     2521 atacgccaga agtccgccgc aggatggtgg agtgcgtgga tcgcttctat ggggagttta
     2581 aaccctggct gaaaaaaacg atcaccaaaa actgggttgg catcactgat aataactacg
     2641 ccaaaaagcg gcaggagatc ctcgattcaa cgcctgaaga gattttttcc tggtatctga
     2701 gtagcgcaga gaaggatttt ttagcccccg attacagtat tttaattatg gggaaaagaa
     2761 tttttcataa tgacaataat cgttcagtca t
//
LOCUS       DAZKJR010000279         2742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKJR010000279 DAZKJR010000000
VERSION     DAZKJR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.281"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            107..295
                     /locus_tag="ACHOWS_005146"
     CDS             107..295
                     /locus_tag="ACHOWS_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFQ0050565.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            439..1284
                     /gene="mscS"
                     /locus_tag="ACHOWS_005147"
     CDS             439..1284
                     /gene="mscS"
                     /locus_tag="ACHOWS_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFQ0050566.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            1410..2630
                     /locus_tag="ACHOWS_005148"
     CDS             1410..2630
                     /locus_tag="ACHOWS_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFQ0050567.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
BASE COUNT          682 a          514 c          753 g          793 t
ORIGIN      
        1 ggatgcttct ggtcagaaaa gggcggttgt gaagcagggc agcttatttc cggtaatgtt
       61 aaacgcagcc tctgattcgt taatttcata atcataagga gtttttatgc gtctgttgat
      121 tgcttttctt cttccctggc tgactttttt caccatcggc cggcctctga cggggatttt
      181 ttgtctggtg ttacagataa cgctgctggg ctggataccg gcaacaatat gggccgtgta
      241 tgcgctttcg cagtataaga cggaacagaa aatccgcgaa gccacgggga aatgatgact
      301 gcagaagagc aggttgtcat accggtgtat atgatgcggg ataaaggccc ggagattcct
      361 ggaaataaaa tgttccgggg aatataaaac agggaaaaac cggatgtgat ggacgcttct
      421 ggcgaggcgg tttcatgaat tgtccgtaat caggatatgt tgcccggtta cgccgcgaat
      481 cttgtggcag cagtggttat tattatcggg ggtatcgctg cacggatgat ttctggtgtg
      541 ctgaataatc tgatgctggg caggaatatt gatgccacag tggcgctgtt cctttccgcg
      601 ctgatacgtc atgccattat agcctttacg ctgattgctg ccctgggacg tgtgggggtt
      661 cagacagctt ccgtaattgc agtcctgggg gctgcaggtc ttgctgttgg acttgccctg
      721 caggggtcat tgtcaaacct tgcagctggt attcttctcg tgatgttccg tcctttcagg
      781 gctggtgaat atgctgacct gggtggtgtt gccgggactg tgcagaatgt acatatattc
      841 tcaaccacga tgcgtacact ggatggtaaa attgttgtca ttccgaacgg gaagattatt
      901 gccggagaga ttgttaattt ctccagggag cctgaacgac gaaatgagtt tatcatcagt
      961 gtttcatatg atgcagatat tgactgtgtg aaacaagtcc tgacagatat tgtgatgtca
     1021 gaagagaggg ttctgaagaa cagggaaatc actgttcgcc tgaatgagcc tggggagtca
     1081 tccatgaatt ttgtagtacg ggtatggagc aggcgtgatg accttcagtc tgtatactgg
     1141 gatgtactgg agagaattaa gcgggagttt gattcagaag ggataagttt tccatatcca
     1201 caaatggata tccatcttgt tcgctccggg aagcaggcag agtcggtaac ccataacagt
     1261 cgctcagtca gttccagaca gtaaaatgaa aagcgcattt tatttgtgaa agatgaacag
     1321 cctgaatctg tcaggctgtt ttcattgtgt ttttatggtc atttttggta tatgtactgt
     1381 atataatgtc atctgtacaa ggatataaaa tgccaaagaa aataatgaca gggattgatg
     1441 tttttgacgc tgccatggag cgtattgaat gggtgtttga tacgttttca tcagtttgtc
     1501 tttctttttc cggcggaaaa gactccactg tcctgtttca cctgatggcg aatattgccc
     1561 gccgaaaaaa acgccgtttt tctgtactgt ttgttgactg ggaggctcag tatcagtgta
     1621 ccattgagca tgtcaccagg ttgagggaaa tgtacgatga tatcacagat acttttttct
     1681 gggtggcgct gcctctgacg accgttaatg gagtgtctca ggtacagcct gagtgggtat
     1741 gttgggagga cggggctgaa tgggttcgcc agccaccaga agatgccata accagagcgt
     1801 ctttttttcc tttttatcag aatgcgatga catttgaaga gtttattccg gcatttaatc
     1861 attggtttgc ccgggaaaaa gggatggttg ctgcgattct tactggtgtt cgcgctgatg
     1921 agtcactgaa tcgttttatg ggactggtat ccggacgtaa gctccgttat gcggaggata
     1981 aaccctggac cacggcatcg gctgagggat tttactacac catgtatcct ttgtacgact
     2041 ggaaaacaag agatgtatgg atttaccatg cgagaacacg tgctgcctat aactctcttt
     2101 atgacctgat gtacagggct ggagttcctc ttcgcaatat gcgggtgtgt gagccttttg
     2161 gaccggagca acgtaagggg ttgtggcttt atcatgtact ggagccggat acatggggca
     2221 gaatgtgtgc tcgcgtatca ggtgctgtag ggggagcgct ctatgcaaat gaaagtggtg
     2281 cttattttgc tttgaggaaa cagataacaa aaccagttca ccatacatgg cagagttatg
     2341 caatgtttct gctggagtca atgcctgaga aaacggcgga gcattacaag aataagattg
     2401 ctgtttatct tcggtggtgg cagtctcatg gatggccgga tggcatccct gatgagcagg
     2461 ataatgacca gggatgtaaa gatattccct cctggcgtcg catctgtaag acgttactga
     2521 aaaatgattt ctggtgtcgg atgctggcgt ttagtccgaa taagccccga cattatgagc
     2581 gctacctggc cagaatgcgt gaaaaaagga aagagtgggg aatattgtaa attatattta
     2641 aacatcagaa atatgacggt taatccagga tagtagctaa aaaaataaga ttccttgctg
     2701 atttatccct gtttgatatg taaaatctga ggggcttata ag
//
LOCUS       DAZKJR010000280         2712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKJR010000280 DAZKJR010000000
VERSION     DAZKJR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.282"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2530
                     /locus_tag="ACHOWS_005149"
     CDS             <1..2530
                     /locus_tag="ACHOWS_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFQ0050568.1"
                     /translation="FGQMTAVHREEGVSLYRRYDSRGQLISVKDAQGRETRYEYNAAG
                     DLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSY
                     DALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVILWYYDESDRITHRTVNGEPAE
                     QWQYDGHGWLREISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETKQA
                     YNEQGLTNRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLLEYMRDRLHRETVRSFGSMA
                     GNTAAYELTSTYTPAGQLQSRHLNSLVFDCDYNWNDNGDLMRISGPRQTREYGYSATG
                     RLASVRTTTSDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAEDAHYVYRHDE
                     YGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGR
                     RMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDHLTTVQTDTTRIQTVYEPGSFTPLI
                     RIETDNGEREKTQRRSLAEKLQQEGSEDGHGVVFPAELVRMLDRLEGEIRADRVSSES
                     RQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISTEGATEWRGEYDEW
                     GNLLNEENPHHLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNL
                     YNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAF
                     AKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGG
                     HFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWVN"
     gene            2542..>2712
                     /locus_tag="ACHOWS_005150"
     CDS             2542..>2712
                     /locus_tag="ACHOWS_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFQ0050569.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQIGKYSDIYK
                     ITSSDFQTATSEK"
BASE COUNT          681 a          663 c          849 g          519 t
ORIGIN      
        1 cttcggccag atgacggcgg tccaccgtga ggaaggtgtc agtctttacc gccgctatga
       61 cagccgtggt cagttaatct cggtgaaaga cgcacagggc cgtgaaacgc ggtatgaata
      121 caacgccgca ggcgacctga ctgccgttat caccccggac ggcaaccgga gcgagacaca
      181 gtacgatgcg tggggaaagg cggtcagcac cacgcagggc gggctgacgc gcagtatgga
      241 gtacgatgct gccggacgtg tcatcagcct gaccaacgag aacggcagtc acagcgactt
      301 cagctacgat gcgctggacc gcctggtaca gcagggcggc tttgacgggc ggacgcaacg
      361 ttatcattat gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcatcct
      421 ctggtactac gatgaatcgg accgtatcac tcaccgcacg gtgaacggcg aaccggcaga
      481 gcagtggcag tatgatggcc acggctggct gagagaaatc agccacctga gcgaaggcca
      541 ccgtgttgcc gtccactatg gctatgacga taagggccgt ctgaccgggg agcgccagac
      601 ggtggagaac ccggagacgg gggaactgct gtggcagcat gagacgaaac aggcatacaa
      661 cgaacagggg ctgacaaacc gcgtcacgcc ggacagcctg ccgccggtgg agtggctgac
      721 ctatggcagc ggttatctgg cgggcatgaa gctgggcggg acgccgctgc tggagtacat
      781 gcgggacaga ctgcaccggg agacggtgcg cagcttcggc agcatggcag gcaatacagc
      841 cgcatatgaa ctgaccagca cttacacccc cgcaggccag ttacagagcc ggcacctgaa
      901 cagcctggtg tttgattgtg actataactg gaatgacaac ggcgacctga tgcgcatcag
      961 cggcccgcga cagacgcggg aatacggcta cagtgccacg ggcaggctgg cgagcgtgcg
     1021 cacgaccaca tcagacctgg atatccgcat cccgtatgcc accgacccgg cgggaaaccg
     1081 cctgccggac ccggagctgc acccggacag tacactcaca gtgtggccgg ataaccgcat
     1141 cgcggaggat gcgcactatg tctaccgcca cgatgaatac ggcaggctga cggagaagac
     1201 ggaccgcatc ccggcgggtg tgatacggac ggacgacgag cggacccacc actaccacta
     1261 cgacagccag caccgcctgg tgttccacac gcggatacag catggcgagc cactggtcga
     1321 gagccgctac ctctacgacc cgctgggacg gcgaatggcg aaacgggtat ggcggcggga
     1381 gcgtgacctg acggggtgga tgtcgctgtc gcgtaaaccg gaggtgacgt ggtacggctg
     1441 ggacggcgac cacctgacga cggtgcagac cgacaccacg cgtatccaga cggtatacga
     1501 gccgggaagc ttcacaccgc tcatccgcat cgaaacagac aacggcgagc gggagaaaac
     1561 gcagcggcgg agcctggcgg agaagctgca gcaggaagga agcgaggacg gccacggtgt
     1621 ggtgttcccg gcagaacttg tgaggatgct ggacaggctg gagggggaaa tccgggcaga
     1681 ccgcgtgagc agtgaaagcc gccagtggct ggcacagtgc gggctgacgg tggagcggct
     1741 ggcggcacag atagagccgg tttatttacc tgagcgaaaa attcatcttt atcactgtga
     1801 ccaccggggg ctgccgctgg cgctcatcag taccgaagga gcaacggaat ggcgtgggga
     1861 atatgatgaa tggggcaacc tgctgaatga ggagaacccg catcacctgc accagccata
     1921 ccgtctgccg gggcagcagc atgatgagga atcaggactg tactataacc ggaaccggta
     1981 ctatgacccg ctacagggga ggtatattac acaagacccc attgggctgg aggggggatg
     2041 gaacctgtat aattacccac tgaatccgat aataaggatg gatcctttgg gtttgtataa
     2101 tttatatcaa ttattatatg atgtttggca tgatgattca tatggaacat catcaattga
     2161 tattactggc agtggagatc taatatcatt aggtggtcat gcaggacttg gcgttgcgtt
     2221 tgctaaaaag aaaggtgaaa tgttatctga tatttgtatt tatgctacag catgtggaca
     2281 tgcaggaatt ggtggtggga taaatgcggc tatcacatat tctgagacca agtctttacc
     2341 tacatcggga gtcagtaatt cagtaggtgt aacggttggc ggcggagttg gggggcattt
     2401 tgcgtatact tatgtagtgg atgttgataa tccagaatca tcgacagaat ctgttggtat
     2461 cggtgcaggt gttgacgctt cagttatgac tctggcttgt agaacgtggc aagaatgctg
     2521 ggtcaattaa caggaggaaa tatgaaaaaa tatatatttt tagttttatt tttattgtgt
     2581 ggttgttctc cagtggacat agagccagct agacaagaag ttaatatctt tcgtggttta
     2641 tatcagatag ggaaatacag tgatatatat aaaatcactt caagtgattt tcagactgcc
     2701 acatctgaaa aa
//
LOCUS       DAZKJR010000281         2703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKJR010000281 DAZKJR010000000
VERSION     DAZKJR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.283"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..434
                     /locus_tag="ACHOWS_005151"
     CDS             <1..434
                     /locus_tag="ACHOWS_005151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050570.1"
                     /translation="IFNTSTQVGYGGVIKVLPVFTLIQTTIFSIPGVALFPAVIGTAI
                     LSGIVGSTSGGVGLVMVTFGQDLLELSQAQNISPGLMHRIIVFSASTLDTLPHSGFII
                     TLLGVCGLSHKQSYKELFIVTCVFPAFAVLSMLVYAFLFLI"
     gene            447..2012
                     /locus_tag="ACHOWS_005152"
     CDS             447..2012
                     /locus_tag="ACHOWS_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFQ0050571.1"
                     /translation="MSIYSRKQCTLEDAVNMIHDNDCVILGGFIGAVVPECIEKGIED
                     RFLKTGKPRNLKIIFAAGQGDAGTKAVNHLSHEGLVTSAIGGHWGLIPKLQKLANEGK
                     ITGYNLPQGVICHLFRDSAAGKLGTLTHVGLGTFVDPRVEGGKINDKTQEDIVSVTEI
                     NGVENLFYKKIDANIAILRGTTSDQSGNITMEDECLILENLAAAQLVHNNGGKVIVQV
                     KQIVRDGTLDPLQVRIPGIYVDAVVVSEDEYHMQTFAEYMNFSYVGKGVKEVKDKETR
                     PLDIKKIIARRAAMELKKNAVVNYGIGAPEVIAEVTDEEGITSELIATVEPGAIGGTP
                     AGGLSFGASSFPQAIITQDQMFDFYDGGGLDQAFLGLAETDEKGDLNVSKFGTKIAGC
                     GGFINITQNAKKVYFCGSFTAGKNKIYVSNGKLHIKEDGSVKKFIKKVQQITFSSDTA
                     RKNNKEVFYITERAVFRLSQKSIELIEIAPGVDLQKNIIECMEFVPEISPSLKIMDER
                     IFLEQPMGLELKD"
     gene            2015..2431
                     /locus_tag="ACHOWS_005153"
     CDS             2015..2431
                     /locus_tag="ACHOWS_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HFQ0050572.1"
                     /translation="MVYTIDKLHLGQKASFEKTITEADVLIFSGISGDINSVHINEEA
                     AKKSIFKKRVAHGVLVSSLTSAVLGMKLPGEGTIYLRQENNFRKPVFIGDTVKATCEV
                     VDINKEKNIVKLSTITTNQNDEVVIEGIAVVMAPKS"
     gene            2457..>2703
                     /locus_tag="ACHOWS_005154"
     CDS             2457..>2703
                     /locus_tag="ACHOWS_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HFQ0050573.1"
                     /translation="MIIKPVIKGVVSKSAHPEGCRHAIMEQIKYCQSHLQQINNGPKR
                     VLILGASSGFGLAARISLTFGGAKCDSIGVSFEKGPSE"
BASE COUNT          850 a          414 c          594 g          845 t
ORIGIN      
        1 caatatttaa tacatctacc caagttggtt atggtggggt aattaaagtc cttcctgtgt
       61 ttacccttat tcaaacaaca attttttcta ttcctggagt agcactattt cctgcagtta
      121 taggtacagc aattctttcg ggaatagtgg gctcaacatc aggaggggtg ggattagtta
      181 tggtaacgtt tggtcaagac ttattagaac tatctcaagc tcagaatatt tcgccaggtt
      241 taatgcatcg tattattgtt tttagtgcat caacgttgga tacgcttcct cattctggct
      301 ttattatcac attgctagga gtttgtggct tatctcataa gcaaagttat aaagagcttt
      361 ttattgttac ttgtgtgttt cccgcatttg cggttctctc catgttggta tatgcattct
      421 tatttttaat ttaaaagagg tttaatatgt ctatttattc aagaaaacaa tgtacactag
      481 aagatgccgt aaatatgatt catgataatg attgtgttat tcttggcgga tttattggtg
      541 cagttgttcc tgagtgtata gaaaaaggca tagaagatcg ttttctgaaa acgggaaaac
      601 caagaaatct caagattatt tttgctgctg gtcaagggga tgcaggaaca aaagctgtca
      661 atcatttatc tcatgagggg cttgtcacta gtgccattgg cgggcattgg ggactaatac
      721 ctaaattgca gaagttagct aatgagggaa aaattacggg ttataatctt cctcaagggg
      781 ttatttgcca tttattccga gatagtgctg ctgggaagtt agggacatta actcatgttg
      841 gtttaggtac atttgttgat ccccgcgtag agggcgggaa aattaatgat aaaacacaag
      901 aggatattgt gtccgtcaca gaaattaatg gtgtggaaaa cctattttat aagaagattg
      961 atgctaacat agcaatcctc agaggtacta catctgatca gagtgggaat ataacaatgg
     1021 aggatgaatg tttgattctt gaaaacctgg ctgctgccca gttagtccat aataatggag
     1081 ggaaagttat tgttcaggta aaacaaatag tacgtgacgg tacattagat cctttacaag
     1141 tcagaattcc aggtatttat gttgatgctg ttgttgtttc tgaagatgaa tatcatatgc
     1201 aaacttttgc ggagtatatg aacttcagct atgtcgggaa aggtgtaaaa gaggtaaaag
     1261 acaaagaaac acgccctctt gatataaaaa aaattatagc tcgacgagcc gcaatggaac
     1321 tgaaaaaaaa tgcagttgta aattatggca taggggcacc tgaagttata gctgaagtaa
     1381 cagatgaaga ggggattacc tccgaactta tcgcaactgt tgaaccaggt gcaattggag
     1441 gaacgcctgc tggaggtctg tcatttggag cttcttcgtt ccctcaggcg ataattactc
     1501 aggatcagat gtttgatttc tatgacggtg gtgggcttga tcaagcgttt cttggattag
     1561 ctgaaacaga tgaaaaagga gatttaaatg tttctaaatt tggaacaaaa attgcagggt
     1621 gcggaggatt tattaacata actcaaaatg ctaaaaaagt atatttctgc ggttctttca
     1681 ctgctggaaa aaataagatt tatgtcagca atgggaaatt gcatataaaa gaggatggga
     1741 gtgtaaaaaa attcattaag aaggttcaac aaatcacttt ctcgtcagat actgcaagga
     1801 aaaataataa agaagtgttt tatattacag agcgtgctgt ttttagactt tctcaaaaat
     1861 ccattgagtt gattgaaata gcgcctggag tcgatttgca aaaaaatatt attgaatgca
     1921 tggagtttgt tccagaaatt tctccgtctt tgaaaattat ggatgagcgt atttttttgg
     1981 aacagccgat gggattagaa ctaaaggatt aatcatggta tacactattg ataaactaca
     2041 cttaggtcag aaggcgagct ttgaaaaaac aataacagag gcagatgttt taattttttc
     2101 tggtatttct ggcgatatta attctgttca tattaatgag gaagctgcaa agaagagtat
     2161 tttcaagaaa cgagtagctc atggtgttct tgtatcatct ctaacatcag cagtattagg
     2221 tatgaaactc ccaggtgaag gcacaatata tcttcgtcag gaaaacaatt ttcgcaaacc
     2281 tgtttttatt ggtgacactg ttaaagcaac ttgcgaggta gttgatatta ataaagaaaa
     2341 aaatattgta aagctttcaa caatcacaac aaatcaaaat gatgaggttg taatcgaagg
     2401 cattgcagta gtcatggctc caaaatctta atttatttaa ctatcaagga ccttatatga
     2461 ttataaaacc tgtaattaaa ggtgttgtct cgaaaagtgc tcatccagag ggatgtcgtc
     2521 atgcgataat ggaacagatt aagtattgcc agtcccatct tcagcaaatt aataatggcc
     2581 cgaaacgagt tctgattctc ggggcatctt caggatttgg tcttgctgca aggatttctc
     2641 tcacttttgg cggcgcaaag tgcgattcta tcggagtttc gtttgaaaaa ggtccctctg
     2701 aga
//
LOCUS       DAZKJR010000282         2702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKJR010000282 DAZKJR010000000
VERSION     DAZKJR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.284"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..356
                     /locus_tag="ACHOWS_005155"
     CDS             51..356
                     /locus_tag="ACHOWS_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050574.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            411..1775
                     /locus_tag="ACHOWS_005156"
     CDS             411..1775
                     /locus_tag="ACHOWS_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFQ0050575.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILARHELKQSSKVALPE
                     AMVKGYITRLENQPVQGEVSSPVTLPAQALRSDAPDVQEHQPLPYSRLVYVVSETKTE
                     PTLFLQKSPLTFLKTFVTGVCTALLAVCVAIPGWQFLTQPSPAEQQLRALLAPLPDVL
                     SADQMAQLSRTPSLLNHTSEWITLSGQQITRLTELPPAYNLQRSAQLLQQLRGLFPDN
                     PRVQEMVDNWHKSVRSRALPEEAMTGWNEGMARLQQLAERLNRLDEQRGKYMTVSELK
                     TEVFGIMQAFNRHIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLINRYQMENAGKP
                     KSQP"
     gene            1791..2423
                     /locus_tag="ACHOWS_005157"
     CDS             1791..2423
                     /locus_tag="ACHOWS_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0050576.1"
                     /translation="MEMENTPGRARTPWTIEDVRYLETHYGVKKTREIAAYLNRSATA
                     VRAMAYLVGITTPVAADWSETEIEIIRRDYPAGVALADICARLNGRAPKSVCWMARRF
                     GLKRLGYWSAEEDEILRQYYPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRL
                     WTAEEWNVLSGNMASGLSALMKLLPGRSRSSVQGAMRRLQKMSAQKSVRQ"
BASE COUNT          728 a          627 c          734 g          613 t
ORIGIN      
        1 tatggttccc cttatccgtg tgcttaacag acataaacga gggctgaatg atgaacccgt
       61 tatttaatga catccagatg cgcctttttt atctgaatca tgctccgtat tcctggcact
      121 ggaatgtcag attcaggcca caggaagccg tatatattgg taatgatgca tgccatatta
      181 ccattacctg taatcagtcc ggttttcatt taaccaggga cgggcagcga ctgttcactg
      241 aacgttatat cagaacactc agtgagttat tagcggttct gaagcggcgg tgggatgtga
      301 caccggcaat catccgtgcg gtggaatacc tttcccgggt accggtatca cactgatgtc
      361 ctttccgtaa ctgttaatca tgatttaata cagcaacacc gaatctgccg atgacttctg
      421 aagtgaaact gaaaaccggt ggtgaccccc ggtcattacc ggattatgct gccctgcgtg
      481 atgaaatcag caaactgacc cacccggcac gtccggatgt ggactggcgt tacgtggaaa
      541 cgctctgcct gcgtctgtat gaacacaatg gcgtggaatt acagacggta tcctggtaca
      601 ccattgcccg gatgcataca gcagggttaa gtggtctgaa tgaagggctg gctctgattg
      661 tggcgctgac ccggcatcac tggtcagtga tgtggccgct gaatacccac gcccggctgg
      721 aaattattac cggtctgttc agtcgtctgc aaaaaacgct ccgggccatg ccaccggatg
      781 agcgtgacaa cctgcccctg ctgtatcaga cagaaacatg cctgaaaacg ctgagtgaca
      841 tactggcccg gcatgaactt aaacagagca gtaaagtggc tttaccggaa gccatggtga
      901 agggatatat cacccgtctg gagaatcagc ctgtgcaggg tgaagtatcg tcgccggtaa
      961 cccttccggc tcaggcactc cggtctgatg ctccggatgt acaggaacat cagcccctgc
     1021 cgtactcccg tctggtgtat gttgtttctg aaacaaaaac agagcccaca ctcttccttc
     1081 aaaaatcccc gctcacgttt ttgaaaacgt ttgttacagg tgtctgtacg gcattgctgg
     1141 cggtctgcgt ggccattccg ggatggcagt ttctgacaca gccatcaccg gcggagcagc
     1201 aactcagggc gctgctggca ccactaccgg atgtgctttc agcagaccag atggcgcaac
     1261 tcagcagaac accctcactg ctaaatcata cgtcagagtg gataaccctt tccggtcagc
     1321 agataacgcg actgacagaa ctaccgccgg catacaacct gcaacgcagt gcgcagctgc
     1381 tgcagcaact gaggggactt ttccccgaca atccccgtgt tcaggaaatg gtggataact
     1441 ggcataagag cgtaaggagc cgcgcgctcc cggaagaggc gatgacgggc tggaacgaag
     1501 gcatggcccg tttacagcaa ctggctgagc gcctgaaccg tctggatgaa cagcggggaa
     1561 aatacatgac ggtcagtgaa ctgaaaacgg aggtgtttgg catcatgcag gcttttaacc
     1621 ggcacatacc ggcagaagag cagttacgtc gttatgatga agcccgtaac cagaatggca
     1681 gtgaacagca gcaaaaacag gctgaaatgg cgctgaatca gttgattaat cgttatcaga
     1741 tggagaacgc aggcaaaccg aagagccagc cataatggct gagataaaac atggaaatgg
     1801 aaaatacccc cggaagagcg cgcacaccat ggacaatcga agatgttcgc taccttgaaa
     1861 cgcattatgg cgtgaagaaa acccgcgaaa tagcggcata cctgaacaga agtgcgacag
     1921 cagttcgtgc catggcgtat ctggtgggga taacaacacc agtggctgcg gactggtcag
     1981 agacagagat tgagattatt cgcagggact atccggcagg cgttgcgctt gctgatattt
     2041 gtgcccgtct gaacggacga gcgccgaaat ccgtgtgctg gatggcccgc aggtttggtc
     2101 tgaaacgcct gggttactgg agtgcagaag aagatgaaat cctgcggcaa tattatcctt
     2161 cagaaggtgt gaaagtgagc cgacgccttt ccggacgtac agaaaatgca gtgaaactga
     2221 gagcgcggga gctgggtgta aagttcacgg gaaacgggcg attacgtctc tggacggcag
     2281 aggagtggaa tgtgttgtcc ggaaatatgg catcggggct atcggcactg atgaagttac
     2341 tgccggggcg ttcccgtagt tcagttcagg gggctatgag gcgtttacag aaaatgagtg
     2401 cgcagaaatc tgtcaggcag tagccaaaat attcctgcat caggagaacg gcagtggcaa
     2461 taataagcag caaactgata cataatgcag cttctgtaaa cggtaatgat caagtaggga
     2521 tatttttatg ttgactggtt gtgaaaaaat gatcaaaatt taaattaacc tttgcatgag
     2581 attagaatct attaatttta tcaaaggaga ttgtgtgaca atagtaagag attctgttag
     2641 tggtgaaatt gttcatagtc atgatcttcg ggaaatggat gttctttata tacaaaaaac
     2701 ca
//
LOCUS       DAZKJR010000283         2683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKJR010000283 DAZKJR010000000
VERSION     DAZKJR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.285"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..198)
                     /locus_tag="ACHOWS_005158"
     CDS             complement(<1..198)
                     /locus_tag="ACHOWS_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFQ0050577.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIE"
     gene            442..699
                     /locus_tag="ACHOWS_005159"
     CDS             442..699
                     /locus_tag="ACHOWS_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFQ0050578.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(749..1699)
                     /gene="uspE"
                     /locus_tag="ACHOWS_005160"
     CDS             complement(749..1699)
                     /gene="uspE"
                     /locus_tag="ACHOWS_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFQ0050579.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1851..2603)
                     /gene="fnr"
                     /locus_tag="ACHOWS_005161"
     CDS             complement(1851..2603)
                     /gene="fnr"
                     /locus_tag="ACHOWS_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFQ0050580.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT          695 a          627 c          636 g          725 t
ORIGIN      
        1 ttctataaca taattaatga tgctaccaac agcactgata attatggtgt agattaaaaa
       61 ttgcaccgca tggaataaaa atccttttct tttacgattt ccgcggcgaa accaaaagct
      121 catcagaatc aatgctgcgc agctaccgaa aataatgacc agattaagcg cattatttgt
      181 aaacagttca gcgatcattg ttttatcagg ctcctccaga taattgtcgt catgccggaa
      241 acccctggcg gggctatttt accgcgacaa ttcattcaga tcatcaatag tcagggaagg
      301 aagtagcaac attagctaac taatcaatgc gttgattgta aatccagcta agaggtgagg
      361 ttttcagagc agacaacggt gaaatgtcat ggtattgtta cgtttaggta acaagaaatt
      421 tgtctgcaca aggattacat catgattatg gcgaaactga agtcagcgaa agggaagaaa
      481 tttctctttg gtttgttggc ggttttcatt attgcggcgt cggttgtgac tcgcgcgacc
      541 atcggcggcg tgatagaaca gtacaatatt ccgctgtctg agtggacgac atcaatgtat
      601 gtgattcagt catcgatgat ttttgtttat agcctggtct ttactgtgtt gctggcaatc
      661 ccgttgggaa tttatttcct tggcggcgaa gagcagtaag taaaaaatag gcccgataac
      721 tcgggccttg tcagttattg aagagtcgtt aatcgtcttc ttcgtcatcc agttcaacgg
      781 gtgtctgata ctggtcaggt ttaatgacca gcaggtcgca gcgaagatga tcaatcacct
      841 gttccgccgt gttaccaagg aatgctgctg aaataccggt gcgtccgacc gtgcccagaa
      901 ccacaatccc cgcctgtaag tgctccgcca aatcaggaat cacctcttct ggcagacctt
      961 tttctacgtg cgtcatgttt tcattaatgc cgaatttctg ccgcagggct ttcattgcca
     1021 gcaaatgttg cccacgaatg gcatcgttat aaacgctcgg atcaaattcc ggcagttcaa
     1081 tcgcgatatt aattggcgtt accggataag cgccaaccag atgaacttcg gtatggttga
     1141 cttgttccgc cagttcgatc gtctctttga ccagtttttc attgagcgca ttatgatacg
     1201 gctcttcact ggcgagattc accgccacca gcgccttacc cccttccggc cacggctggt
     1261 ctttcaccat ccacacgggg cttgggcatt tgcgtaacag atgccagtcc gttggcgtaa
     1321 aaatcaccgc ttccagacgg tcatgttggt gcgccatttt tagcaccaaa tcgtgcccgc
     1381 cgctgatcac ttcctgaatg atggcttcga aaggacggtt atgccagacc actttaattt
     1441 caatgggaac gccagcattg agataatatt ttgcctgctc gtggatccag gctgtacgct
     1501 ggctgatgac gccctgacgc atagcggtac gttcgtccgg ggagagcagg gtggtcattt
     1561 cgtatgagaa gtcatagatc ggcaaaaagg ctttaatttt gccaccaatc cgttgatgta
     1621 aataaacagc tcgccgcaat gctggttggt cgtcctggtt aggatcgata acaacgagca
     1681 tgttctgata catagccata cagggtctcc ttacaacaac tgtcaacgca gtttgtaatt
     1741 aaaagattaa cccatatctg gtgaatgaaa cagtgatgaa ccttctgcca gatcaataaa
     1801 tcagaaaaat ttaatgatat gacagaagga tagtgagtta tgcggaaaaa tcaggcaacg
     1861 ttacgcgtat gaccagcaag ctgggccagc gcatcgttat tttcgatggt gatgtattta
     1921 cctttgactg ccagcatacc gcttttctgg aagcgaccca gcagacggct gatggtttct
     1981 accgtcaggc ccagatagtt accaatatcg ccacgagtca tcgtcaggcg gaattcacga
     2041 ggggagaagc cgcgttgggc aaaacgacgg gacaggttgt agatgaatgc agccagacgt
     2101 tcctcggcat ttttcttcga caacagcagg atcatgtcct gatcgccttt gatttcaccg
     2161 ctcatcagac gcatcatctg ctgacgcaga ttcggcattt taccggacaa atcgtccagc
     2221 gtttcgaacg ggatttcaca taccatcgag gtttccagcg cctgcgcgaa gctcgggtga
     2281 tggccgctgc cgatggcgtc aaatcccacc aggtcgcctg ctaaatggaa accagtgatt
     2341 tgctcgtcgc cttgctcagt gatggtataa cttttaatcg taccggagcg gatggcataa
     2401 agcgatttaa gttcatcacc agccttaaac agcgtctggc ctttctgaat aggcttcttc
     2461 cgctcaatga tattatcaag ctgatcaagc tcatgttcgt tgagtgtgaa cgggatgcaa
     2521 agctggctga tgctgcaatc ctggcaatgg atagcacaac cgccagactg aatgcgccgt
     2581 ataattcgct tttccgggat cataggtctg ctcaagccgt aattgatatt tgtcaatttt
     2641 aacatctttt tagggagcaa gtaagtctaa gcaaacctta aca
//
LOCUS       DAZKJR010000284         2682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKJR010000284 DAZKJR010000000
VERSION     DAZKJR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.286"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..>2682)
                     /gene="chiA"
                     /locus_tag="ACHOWS_005162"
     CDS             complement(34..>2682)
                     /gene="chiA"
                     /locus_tag="ACHOWS_005162"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFQ0050581.1"
                     /translation="ALPALAMEVWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGKAK
                     ANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQG
                     TVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRYVRA
                     ATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQE
                     GSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQYGNPGSC
                     SVKPDNNSGAVTPVDPTPETPVTPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGSEIY
                     HVIFNGNVYQNAWWVGSEDCPRGTSAENSNNPWRLVRTATAAEMSQYGNPTTCEIDNG
                     GVIVADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSAS
                     TVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSYSNEELN
                     NAPQFNPETLYASDTLIRFNGANYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTPK
                     KAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGMQ
                     NYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRVLDF
                     DIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSDA
                     KAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLHPNKSDA
                     EIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTNLS
                     PEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          618 a          711 c          644 g          709 t
ORIGIN      
        1 atgcggcgat accggaaggt atcgccaaca catttattgc ttagtaaacg gcgcgaagat
       61 ttcgctaaat gcgtacttcg gtgcctgttc tttagtcagg ccgtggaatt ccggagacag
      121 gttagtgccg cctggcaggt cgcgcgcgat tgaccagata ccaaccatac cgagattacg
      181 cttctgcgca tcctgcatga ccagacgagc atcagagaga tagaacacct cgccctgaac
      241 gtcgttcacg ccaaccatcg gcgtggtacc catcatagcg tcaatttctg catcgctctt
      301 attgggatgg aggcctttca attgtgaatg caggttggca atcgcagacg ttgcacactt
      361 accgtgaatg ttctggcctt cggtatttgc agactgacaa atcgcgttac cgtagtccat
      421 tgtcatcacg ttcacacccg ccagctcaac acctttcgct ttggcatcgc tcaggacatt
      481 catcccttcc ggcgtcaggc cagtcggcag aattggcaag gtgtaccaga tagcaatatc
      541 tttgccctct gacttccatt tatcctgcac tttcttcaca gcaaggttac gacgttcaat
      601 agatgcctga tccgcaaccc aggtgccttc gatatcgaag tccaggactc tgaggttcag
      661 gttatcaacg atgtcataat aatgctgcat cagatcgtct acgttcttac aggaagcagc
      721 cagcggagcg ttgttagcac caccgataga cagcatcaca tcgccgccag cttcacgcag
      781 agctttgatt ttgctgtact gagcgtaatt ctgcataccg taagcggtac cccatgtcgg
      841 cagacaggtg ttcgcatctt tactcaccac aaacgccagc gtgaagtggt tgacgttatg
      901 attcttagcc agcgcagcca gatccgggat cgtattcagc gtaaagtcaa catacggtgc
      961 ataaacgtgt ttcggccacg ctttcttcgg cgtacctaca cgttctttgg ttccggtcca
     1021 gtcaacaaaa acacgccacg ggttgctgtc agaaggagaa actttctgca ctttactctg
     1081 agaaatgtag ttcgcaccgt taaagcgaat cagcgtatcg ctggcataaa gcgtttctgg
     1141 attaaactgc ggcgcattat tcagctcttc gttgctatag ctctgagcct tacctaacgg
     1201 cttccacggg cggctattgc taccggtggc gttttggttc gcaaccagag ctgggttgtc
     1261 gctttgcgtc cagaacagcg cttcataagc ctgcccatca acaacaacgc gatcaccttt
     1321 cacgtacacg gtggaagcgg accatgcttt cgccggttcg tatttcttcc acgggttatc
     1381 gccgcccggg acaaagcccc atgcgtcttg atcgacagag gttttataat gtgcatcgtt
     1441 ataatctacg atgctgttcg cgctgtacgc tttgctggcc tggaaaccat ccgcaacaat
     1501 gacgccgcca ttatcaattt cacaggtagt tgggttaccg tactgactca tttccgcagc
     1561 ggtagctgta cgcacgagac gccatgggtt attggagttt tctgcactag tgccacgtgg
     1621 gcaatcttca gaaccaaccc accaggcatt ctgataaaca ttaccattga agataacatg
     1681 gtaaatttcg ctaccttcct ggccactcca cgcctgcagt gagtaatcgt tactgctatc
     1741 cgctggtgtt gatggctcgt tattatccgg agtaggagta actggagtta ccggtgtttc
     1801 cggagttgga tcaaccggag tcacagcacc gctattatta tccggcttaa cggaacagga
     1861 acccggatta ccatactggc tgatttctgt tgccgttgcc gcgcgaacat aacgccatgg
     1921 gttgctggca tcgttttctt tcgcatcgcc tgggcaattt gccgcgccta cccaccaggc
     1981 gttcttataa atgccgccgt tgtagataac gtaccatgta gaagaacctt cctgaccttt
     2041 ccaggctacg acagcatagt tgtcgtttga cttatcagct ggagctggct gaacatcagg
     2101 agccggttta acgtccggtg ccggtttcac atccggcgca ggctgtggtg ctgaagtaca
     2161 ggactgtgga ttactggttt ctgagatttc cgttgccgta gcggcacgaa cataacgcca
     2221 tgggttgctg gcatcattgc ctttcgcatc acccgggcaa ttagaagagg ctacccacca
     2281 ggcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
     2341 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
     2401 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
     2461 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgtcg cggtacgctt
     2521 taaacgccat gggttagttg catcatttgc ttttgctttt cccgggcaat ttgtagaaga
     2581 aacccaccag gcattttcat aaattttgcc atcgaaaata acctgatatt tattaccacc
     2641 ttgttggtta ttccatactt ccattgccaa tgctggcaga gc
//
LOCUS       DAZKJR010000285         2668 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKJR010000285 DAZKJR010000000
VERSION     DAZKJR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.287"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..911)
                     /locus_tag="ACHOWS_005163"
     CDS             complement(<1..911)
                     /locus_tag="ACHOWS_005163"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFQ0050582.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKKMK"
     gene            1029..1581
                     /locus_tag="ACHOWS_005164"
                     /pseudo
     CDS             1029..1581
                     /locus_tag="ACHOWS_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1815..2501)
                     /locus_tag="ACHOWS_005165"
     CDS             complement(1815..2501)
                     /locus_tag="ACHOWS_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFQ0050583.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
BASE COUNT          767 a          672 c          525 g          704 t
ORIGIN      
        1 attttcattt ttttatttct gtcatgttct tcgagcagtc gctctgcaag ttttgataat
       61 acatccagat tcttttcctt taccggagaa aaaggacttc tgcatacagg agtatttata
      121 atccccatat agtaaagaag agtttttata cctgccagga caccactctg aataagagta
      181 tcaataacct ggttacaggc cacctgcatt tccttcgcct taatcacatc attatttttg
      241 actgcttcaa atatttcaag ataacgccac cccattatat tatatgtact accgattccc
      301 ccatctgcac ctgcaattaa ccctgatgca aatatttcat cgtaaccatt atataacaca
      361 agctctggat gattcctttt gatttgttcc atctggaata aatcaccgga tgtctgtttt
      421 aatgcacaaa ccccgggtat tgtgacaagc tcatttatct gatctaaaga aaaacgcact
      481 ccgctaagag ccggtatatt ataaactacc atcggaagtc catctgcaga atcaataatc
      541 ttcctgtaat ggatacaatg ctcttcaaat gaaaaaggat aataaaatgg agtaacagca
      601 gatatagcat gatatccgta tttttttgca gcttttgcaa gtacctcgct ttctgccgta
      661 ctgattccgc ccacatgcgc tataagtgtt agtcttccat ctgattcatc ggctactgtt
      721 tcaagaattt tttctcgctc tgcaacattc tgaagaaatg cttctccagt agagcctcca
      781 acatacaatc ccttaatatt ttgttcgata ttaaaacgga tcagttttcg aagactctcc
      841 atatcgattt gctgggattg gtcataagga gtgggtaatg ctgatataac ccctttaaat
      901 tcacattgca tttttaatca ccttcactta actgcataat gggataccta gtacaccatc
      961 aagtacaaag attatccaac agaaaagaag cacaatgtga agattatcaa aaaaaagcca
     1021 tcagaaagct gtcatgccgc tcagttaagg atcggttgat cgatccagtg tccagttact
     1081 attctctgcg taaccctctg cgactatctt gccctggaac tgattaccca cagccctgaa
     1141 gagtccgaca ctgtcacgct acacaaacgg cgtcttcctc tggaaatgat ggtctggtga
     1201 attatcagta tggcccatga gcgtaaggaa ccttcatcag attgtgaacc ggctggacat
     1261 catgctgcca ggcagtagcg ccgtgctcca tgcccgccag cggccaggaa gcggagccat
     1321 acgctgcgtg ttcactcaaa cagcgcagct ctagcataac accacgccat atccgctctg
     1381 gtgcgatctg aatctgctga ccattgatgg cgtgttctga cgaacacagg acacgcaaga
     1441 acacgataaa gcttcactcg ccaaatacat agtggaaacc agacacttta tcctcaggta
     1501 aaaatggtct gtctgataga actgaccagc catctactga cggcagcggc cttcgataca
     1561 atgaatctgg cactaaataa gactggcaat atagccgata atgcgttgaa tcagtggcta
     1621 tatcgtatac ataaaacaga catcattatt ctgtcttgtt gtccaacaca taaaaactaa
     1681 taaaaacata ccattccctg tcgtcagaga atttattgat aaacaggctt tattgaacaa
     1741 atcaaacatt cagggaactg gcagttgaac aaaacgccag tccgaagact ggcgcagtga
     1801 taaaacattc tgcatcatgc cacattctgc tcctgaagat gacgcagacg atggtcccag
     1861 tttcccaggt aatgcccggg agtcagcagt acgcggcgtc gctcattaca gagcgagtta
     1921 ccaatcacca cttctccggc cacaccacac aacgacagtt gtatatacgc catgccggct
     1981 gccagcgggt caatatccgt tgccgacacc cacatcctgt ggtatgccgg ataccccgaa
     2041 cggttcagga tgtcagccat tgccagaatc atactgccgg caccacaggc aggttcactg
     2101 agcgtgataa agggtttgtt ctcaaacaac gccttcacat ctcccatctg catctgtgcc
     2161 atcatgctgg caatactcca gggcgtgaaa aactgactcc ggtatttatc ccccagttcc
     2221 agttgcatga acacactccc cagaaaatca cagaatccct gctccagtcc ctcaatgaca
     2281 tgagcaagca aatgcgcaat gtggataaca tcctgctttt cataaccact gacgattcgc
     2341 agatacttct gttcccgggc ctcactgaac tgaagccggt tttccagggc aatcacacta
     2401 cagctgacaa agtcttcaaa caccttatga cgtttgtgat aacaggctgt ccggttaaac
     2461 aacgaaatga actccttctg atggtcagtc atggtggcca tgttttctcc gtaaaaacag
     2521 ggaacacatg gcccggacag gggaacaggt gttccccttc gggttgaaat gttgataaaa
     2581 cgggcatcac ggacatgata cccggaggtt aattactggg ctgagaccag aggaaccggt
     2641 aaaggtgcgg ctttcgtctg ttcttcag
//
LOCUS       DAZKJR010000286         2661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKJR010000286 DAZKJR010000000
VERSION     DAZKJR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.288"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..665
                     /locus_tag="ACHOWS_005166"
     CDS             36..665
                     /locus_tag="ACHOWS_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFQ0050584.1"
                     /translation="MSIEQIINEINVYLNSLKKEDERIDAINKIKIELHRSSPFYAEP
                     VDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGF
                     HRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIV
                     RDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
     gene            complement(847..2592)
                     /locus_tag="ACHOWS_005167"
     CDS             complement(847..2592)
                     /locus_tag="ACHOWS_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0050585.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
BASE COUNT          831 a          644 c          531 g          655 t
ORIGIN      
        1 aagaaaaata tacaaattaa aagaaataga aacacatgag cattgaacaa atcataaatg
       61 aaataaatgt ataccttaat tctcttaaga aagaagatga acgcattgat gcaattaata
      121 aaataaagat tgagttacat aggagtagtc ctttttacgc agaacctgtt gattgtgtta
      181 tatggataaa aaacaatcta attgaagcaa acgactataa tcctaacgtc atggctccct
      241 ccgaaaaaag gttattaaca cactccctgt tggttgatgg attcactcaa cctgttgtca
      301 ccttagctca aaaggggaaa tacaccgtta ttgacggatt tcacagacaa atgcttggga
      361 aaagcacaac aaaactagag aaaaaactaa aaggatattt acctgtcaca tgcattaagc
      421 atgataataa ctttaaagct tactctatcg cctcgaccat caggcataac agggccagag
      481 gaaagcacca gatagacgcg ctctccgata tcgtccgaga tctttcccga atgggatggg
      541 atgatctccg aataggtagt gaactgggca tggatacaga tgaggtactg agattaaaac
      601 aaataagtgg gttaacagaa ctatttgagg aggaaaactt tagtccggca tggacaataa
      661 agtaatcatt tagggggtga ctttatccag taattaccga ctgatatatg atcaccccat
      721 gtaatggaca gctattgaca gatcaaaaac atgggggata ttaattcaac tctgaatact
      781 cgctaaatac ccatcttaga ttatcaaaaa acgaatgatt acatatcagc aataataaaa
      841 cacaacttat acaaaatcct caagatgacg actgatactg ttttcacgat aacaggtcac
      901 cagtaattcc cgtgtagccc gggtgaacgc aacataaagc gccggaataa cttcagactc
      961 atccgcagca cctgagtgaa cgaaagaagc attgataacc gcaacatacg gaaactccag
     1021 tcccttactg ctctggtagg tcagtaaatg cacaacatcc ttgcgtcgcg aatatttctt
     1081 cttaccttca ctgtcaaagc aggtggcata aggtatcccg cgttgtgtca tgacctcttt
     1141 cagatgtttc actgaaaact ctgcaggaca caaaacagcc atatcactcc agtgaccgca
     1201 cagtgtatag cgtttctcca gccagcccac aacctgcact gcctcatcac tctcagaagc
     1261 acaacgcaga atttccggct ctgtgccctc ttcaccgccc gcctgtggct gaacaaacgg
     1321 tatttcacgg ttctgatgct tctcaaaata ctcacgggag aatgcataag caaagtgcag
     1381 aatgcgtttc gtattacggt aatttaccgg caggacggaa gtacgtccct gcgcctgaat
     1441 ccccacactg gcaagcgaaa agttcagagc ccgttcccgt ctgtaaatgg actgtgcgtc
     1501 atcgtacatc aggagcagtg aacgggacgc gttatcaaac aggcgggcta tcagagccag
     1561 ccagcgactc tcaaaatcat gcccctcatc cacaagtacg gcatcgtatc cagtatcagt
     1621 aattgttccg ctgttcacgg catcttccag cgcactgaag catttctccg ggtaatcctt
     1681 tcctccagca gtcacctgaa ttccatggcg ctttatcatt gaggcgcacc aactatgaaa
     1741 atggaatacc gtaacccgtg acttcagtcc cctggactca atacattccc gcaggtagct
     1801 ggccagagta atgttgtaac atagcaccag aatcttacca ggggtagttt cagccaagta
     1861 aagacaacgg aacaaaagaa tcattgtttt tccagagccg gctaccccat gaataacacg
     1921 atgcccatca ccaatatttc tggccagaat ctcctgctga atatccatta ttttgatttg
     1981 tgagtgctgt ctgacggtca tttcaggaaa cagatgagca cgcagaatat ctctaacctg
     2041 tggtgtcacc cgggttctga atcccatcgt aaacatcccg gcaatttttt ggcggaatat
     2101 atcaggtaaa acagactgag tcatatcatc ctgacagatt gtctgcgctg aagggaaaat
     2161 cctttccaca gcattttcat cattcccagc cagagccttc agttgctggc gggtaatacg
     2221 ggtaaacaca acaccatacg cccaggccag attcagacgc cccttatact gaccatcatt
     2281 ctgtcgcaaa cgtgagtctg caggtaaagc atttacggta tcacaggcat aacgacgaac
     2341 ctgtagcagc ggatttattt ctgatttaag aataccgtta gtttcaagaa tgacctgttc
     2401 gtgatttacc tgatgcaacg tgtgaacggt ccagtccttg acttcaagaa ataccagacc
     2461 attctccggg tcaataatga caaaatcagg atgtcggttt ttccgtccca ccggaatatc
     2521 gtaccagacc agacactcat tactcataaa tgcctcaaga cgggatgcaa cccgcttttc
     2581 cccggcagtc atccgctcct cggtaacacc tgatatattt atcagtttag ccataaaaca
     2641 gcccctgaca gatgatgaac a
//
LOCUS       DAZKJR010000287         2655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKJR010000287 DAZKJR010000000
VERSION     DAZKJR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.289"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(149..910)
                     /locus_tag="ACHOWS_005168"
     CDS             complement(149..910)
                     /locus_tag="ACHOWS_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFQ0050586.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1700..>2655
                     /gene="subA"
                     /locus_tag="ACHOWS_005169"
     CDS             1700..>2655
                     /gene="subA"
                     /locus_tag="ACHOWS_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFQ0050587.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKMSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEG"
BASE COUNT          760 a          606 c          604 g          685 t
ORIGIN      
        1 caacattatt atgaagagaa taaaataata agaaaaacag aaatatcctg taaagcagat
       61 aacagacaac cctgcagtat aattacggca gggagtttcc gtttaattac agaagacagg
      121 taattattat ctttcaggag tagtttcgtc agaagttata agtcacacca agcatgatgt
      181 catgactttt aacatcaact tctgatttat atttatcgcc ccactcatcc ttgtaactca
      241 cactggcatc acctgcatca agatagcgat agctgaggtc cagagcgata tccggggtta
      301 cgtcatagcg gacacctgcc ccaaaactcc atgcaaaatt gttatcatat cccgggtaat
      361 gcaacgcaga aaaattgctc acctcaccag atttattcca ggaggtatca gtatatgttg
      421 ccttatgatg tatccgtgca taacccagtc caacagacac ccatggagtg aatgcactgc
      481 tggtgaatgc actgctgttt ctgaagtcat aatacgtatt caccatcaga gtattcacac
      541 tgagcctgtt ctgtgtgtct tcccgaccac caccgaccat cggagagtac catgtatcca
      601 gtgtataacg ggagtccgca gctccgcggc catagaattc cacttccgtg cgcacaggaa
      661 cattgtaatg ctgataaaaa tcatagccca ccgcaaggcc ggcaccgaat accgtatcgt
      721 tcttatcgcc gcccttatac ttataaacat cgtccccaaa gtcctgacga aagcgctggt
      781 cagtctgagt cacaaccgaa gcaccagctt taccggtcat ataaaagcct gttttgcctc
      841 tatcagccag ggccaggcct gaaaacatgc ccaccacagc aagcgctgaa accgcaataa
      901 tctttttcat ttcagttatc tccgctatca ctttccccga acgataaacg tggttgctta
      961 ttctaaaccc atgcaaaaga cagggtaaaa agagaaaaat cgatcgaatc gatcgacaaa
     1021 atcgatcatg catgatgaag atttcttatc gaatacataa aaatagtgat ggcaatcgtg
     1081 gcaggagctt acgtcaggcc ctgaagtact gacacaggca ttgatggcag gtgatataag
     1141 gagtgctcca gggaatgact cccgactgac ccaggcactg tcccgttgtc aacccgtatg
     1201 gttactgctc tgactcattt cctgtcgtca gacgttcaac tctggaccac tggttatgac
     1261 gacaggtgtc gctcatttgt cagcactata acggtcaaag atcgtggcat tgacacattc
     1321 tccagaggta aatttctcaa gaggtaacaa ctctgccagg aggatatctg cagagagtta
     1381 cattcgtttg ggttcatatc tgtgccgcgc gttttccttc catcagtgaa caaacattca
     1441 gtcccatgac gaccgggcaa atagcgtgtt cacagatatt gctgtccagg tacgaagtac
     1501 cataatggat attggtaagt tattgggtga cccccctcca tgaaaaaacc aaatataaaa
     1561 atgaatgttt cgggagagac ttcactcgct ttattgatag tgattgttta tcttttactg
     1621 aagacaggat atttcagatg tgatatccct gtaacatatt gaccagcaat actttttatt
     1681 tgtaccactg gaggatattg tgctgaaaaa tttacggctg catattctgt tcctgctttt
     1741 tatctcagtg tcagtcaggg cggaaaaacc atggtatttt gatgcaattg gtctgacaga
     1801 aacaaccatg tcgctcacag acaaaaatac cccggttgtc gtatctgttg ttgattccgg
     1861 agtggcattt gtaggaggcc tcagcgacag cgaatttgcc aaattcagtt ttactcagga
     1921 tggttcgcca ttcccggtaa aagagcctga agctttatat attcatggta ctgccatggc
     1981 ttcactcatt gcctcacgtc atgaggttta tggtgtttat cctcatgcac tgatatccag
     2041 cagaagggtt attcctgacg gtgtacagga ctcatggatt agagcaactg aaagcattat
     2101 gtcaaatgtt tttcttgcac caggagaaga gaaaatcatt aatatatcgg gaggccagaa
     2161 ggggatatct tccgcatcgg tctggtccga actgctttcc cgtatgggcc ggaataatga
     2221 gcgattaatt gttgcggcag tgggtaatga tggcgcagat atacgaaaaa tgagtgctca
     2281 gcagagaata tggcctgcgg cttaccatcc ggtcagcagt gtgaataaaa agcaggatcc
     2341 tgtgataaga gtggcagctc tggcacagta ccggaaagga gaaacacctg tattgcatgg
     2401 tggaggggtc accggaagtc ggttcggaaa cggctgggtt gatattgccg caccgggaca
     2461 gaatattaca ttcctcagac cagatggaaa aacggggatc ggtagtggaa cctcggaagc
     2521 aacagctata gtttccggcg ttctggcagc gatggtctca tgtaatcccc gtgcaacagc
     2581 gacagaactg aagcgtacac tgctggagtc tgcagacaaa tacccctcac ttgcagataa
     2641 ggtgacggaa gggag
//
LOCUS       DAZKJR010000288         2635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKJR010000288 DAZKJR010000000
VERSION     DAZKJR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.290"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            181..1794
                     /gene="mppA"
                     /locus_tag="ACHOWS_005170"
     CDS             181..1794
                     /gene="mppA"
                     /locus_tag="ACHOWS_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFQ0050588.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAQTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPVYDKVLAQASTENTVKARNADYNTAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1845..>2635)
                     /gene="ynaI"
                     /locus_tag="ACHOWS_005171"
     CDS             complement(1845..>2635)
                     /gene="ynaI"
                     /locus_tag="ACHOWS_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFQ0050589.1"
                     /translation="CTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKIT
                     IIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGD
                     WIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTI
                     GLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVW
                     AEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPDQGR"
BASE COUNT          761 a          588 c          623 g          663 t
ORIGIN      
        1 gagttctatg tgctttaccg cattttggag actattattt acactaaatc tgatttgata
       61 tattgatact taaaacattt gatgcttcct ttgtcacttt tttgatggaa gttgtttgca
      121 tttctttaag gcgaaacaaa taattacgca tcaattttaa tgtcggttag agggaaactt
      181 atgaagcact ctgtttcagt cacgtgttgt gcgctgttgg tcagcagcat ttctctttcg
      241 tatgctgctg aagttccgag cggcacagta ctggcagaga agcaggagct ggtgcgccac
      301 attaaagatg agcctgcgtc gctggatccc gctaaagccg tgggcctgcc agagattcag
      361 gtcattcgcg atctgtttga aggactggtg aatcagaacg agaaagggga gattgtcccc
      421 ggcgttgcga ctcagtggaa aagtaatgac aaccgtatct ggacttttac cctgcgcgat
      481 aacgcaaaat gggcggatgg cacaccggta acggcgcaag attttgtcta cagctggcaa
      541 cgtctggtgg acccaaaaac attgtcgcca tttgcatggt ttgccgcgct ggcgggaatc
      601 aacaacgcac aggcgattat tgatggtaaa gccgcgcctg accagcttgg cgtcaccgca
      661 gttgatgccc aaactttgaa aatccagctt gataaaccgt tgccgtggtt tgtgaattta
      721 accgctaact ttgccttctt cccggtgcaa aaagccaacg tagaaagcag taaagagtgg
      781 acgaaacccg gaaatctgat cggtaatggc gcttatgttc ttaaagagcg cgtagtcaat
      841 gaaaaactgg tcgtggtacc gaatacccat tattgggata acgccaaaac ggtgctgcaa
      901 aaagtgacct tcctgccaat taatcaggaa tccgcagcca ctaagcgtta tctcgcgggg
      961 gatattgata tcaccgaatc cttcccgaaa aatatgtatc agaagctgtt gaaggatatt
     1021 ccggggcagg tttatacgcc gccacagctc gggacctatt attatgcgtt taacacgcaa
     1081 aaagggccga cggccgatca gcgtgttcgc ctggcattaa gtatgacgat agatcgccgc
     1141 ctgatgaccg aaaaagtgtt gggaacgggc gaaaagccag cgtggcattt tacaccagat
     1201 gttactgcag gatttacgcc ggaaccttcg ccgtttgaac aaatgagtca ggaagaactg
     1261 aatgcgcagg caaaaacttt gttgagcgca gctggttatg gtccgcaaaa accgctgaag
     1321 ctgacgcttt tgtataacac ttcagaaaac catcaaaaaa ttgcgattgc tgtagcatca
     1381 atgtggaaaa agaaccttgg cgtagatgtt aaattgcaaa atcaggaatg gaaaacctat
     1441 atcgatagcc gtaacaccgg caattttgat gttatccgag cctcgtgggt aggggattat
     1501 aatgaaccct ccactttcct gacattatta acgtcaacgc attcaggaaa tatttcacgc
     1561 tttaacaatc cggtatacga caaagtactg gcccaggcat caacggaaaa taccgttaaa
     1621 gcgcgtaatg ccgattacaa tacggcagaa aaaatcctca tggagcaagc accgattgca
     1681 ccaatttatc aatataccaa tggacgatta atcaagccgt ggctgaaagg ttatcccatt
     1741 aataacccgg aagatgtggc gtacagtcgg actatgtata ttgtgaagca ttgatgtgat
     1801 gggaactggc gttacccttg tggataacgc cagtgatgtc tgatttagcg accctggtcc
     1861 ggcggtgtaa tgttatccat atacagcgtc tggctgggga aggcaaagtc cgcgccgtgt
     1921 gactgtacaa tatcgataat cttcaaataa acgtcttgct gtgcagcaag ccattcagcc
     1981 cataccgtgg ttttggtaaa gcaataaacc ataatattca atgaagagtc agcaaactgg
     2041 ttgaaataaa ccagtaaggt ttgtcgctgg tcgatggccg ggtgattttt cagcatctca
     2101 cgtacggctt cgacaataac gcccactttt gccgcatcct cataacgtaa accaatggtc
     2161 gtggtaatgc gccggttggt cattcgtcct gggttttcta cgctgatcga cgaaaacagc
     2221 gagttcggta cgtacaacgg acgattatca aaagtcgtaa ttttggtaat tcgccagcca
     2281 atttccgcta ctgtaccttc gatatttctg tccggtgaac ggatccagtc gccaatacta
     2341 aaaggacggt cgaaatagag cataatcccg gaaaagaagt tactcaaaat atctttaccg
     2401 gccataccga cagccagacc accaatacca ccaaaggtca acaagccaga aaggctcata
     2461 ccgaaatgtt caccataaag cagaacaagc accacaataa tggtgatttt gatgatacgc
     2521 gacataatcc gcgcactggt gatatcgcga ccttttttaa tctgctgttt ttcaaactga
     2581 tttatcagca gaaatagctt aatcgtcaga ataaccgcaa tcagggacgt acaga
//
LOCUS       DAZKJR010000289         2604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKJR010000289 DAZKJR010000000
VERSION     DAZKJR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.291"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..180
                     /locus_tag="ACHOWS_005172"
     CDS             <1..180
                     /locus_tag="ACHOWS_005172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050590.1"
                     /translation="PVGSPERTRKNDNNYHLHVTTCIYAIKPRQIINYDAGIVLIDLH
                     QLNVKTTSDNTNQRH"
     gene            196..390
                     /locus_tag="ACHOWS_005173"
     CDS             196..390
                     /locus_tag="ACHOWS_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFQ0050591.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            751..1044
                     /gene="iss"
                     /locus_tag="ACHOWS_005174"
     CDS             751..1044
                     /gene="iss"
                     /locus_tag="ACHOWS_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFQ0050592.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(1135..1317)
                     /gene="rzoD"
                     /locus_tag="ACHOWS_005175"
     CDS             complement(1135..1317)
                     /gene="rzoD"
                     /locus_tag="ACHOWS_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFQ0050593.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1534..2031)
                     /gene="rrrD"
                     /locus_tag="ACHOWS_005176"
     CDS             complement(1534..2031)
                     /gene="rrrD"
                     /locus_tag="ACHOWS_005176"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFQ0050594.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(2031..2246)
                     /gene="essD"
                     /locus_tag="ACHOWS_005177"
     CDS             complement(2031..2246)
                     /gene="essD"
                     /locus_tag="ACHOWS_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFQ0050595.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          743 a          627 c          556 g          678 t
ORIGIN      
        1 cctgtcggat cgccggaaag gacccgcaaa aatgataata attatcatct acatgtcaca
       61 acgtgcatct acgccatcaa accacgtcaa ataatcaatt atgacgcagg tatcgtatta
      121 attgatctgc atcaacttaa cgtaaaaaca acttcagaca atacaaatca gcgacactga
      181 atacggggca acctcatgtc aacgaagaac agaacccgca gaacaacaac ccgcaacatc
      241 cgctttccta accaaatgat tgaacaaatt aacatcgctc ttgagcaaaa agggtctggg
      301 aatttctcag cctgggtcat tgaagcctgc cgtcggagac taacgtcaga aaagagagca
      361 tatacatcaa tccaaagtga tgatggatga acatcccggt ttcttccacc atcgcaccgg
      421 aaaagcgact atgagggtaa ccctgcgtct gtcagcacag taaaacccgg tgtgcatcgt
      481 ttttgattat tcccgcacac tcacgcagaa ggaattcccc gtcgggctac ggtcatggtt
      541 aatgcgggaa tacggcgacg atacagcgca gctaaaaggg taatggacag atagagcggt
      601 ttatttcatt ccacaggatt ctgagtgccc cccctcctcc aataggctga gcatccacct
      661 atatagtttt aattttcatc aatccattta actatcgttt aattgttgtc acataggatt
      721 ctgccgtttt taacaatgca ggataataag atgaaaaaaa tgttgttttc tgccgctctg
      781 gcaatgctta ttacaggatg tgctcaacag acgtttactg ttggaaacaa accgacagca
      841 gtaacaccaa aggaaaccat cacccatcat ttcttcgttt cgggaattgg acaggagaaa
      901 actgttgatg cagccaaaat ttgtggcggc gcagaaaatg ttgttaaaac agaaacccag
      961 caaacattcg taaatggatt tctcggtttt attactttag gcatttatac tccgctggaa
     1021 gcgcgtgtgt attgctcaca ataattgcat gagttgccca tcgatatggg cagctctatc
     1081 tgcactgctc attaatatac ttctgggttc cttccagttg tttttgcata gtgatcagcc
     1141 tctctctgag ggtgaaataa tcccgttcag cggtgtctgc cagtcggggg gaggctgcat
     1201 tatccacgcc ggaggcggtg gtggcttcac gcactgactg acagactgct ttgatgtgca
     1261 accgacgacg accagcggca acatcatcac gcagagcatc attttcagct ttcgcatcag
     1321 ctaactcctt cgtgtatttt gcatcgagcg cagcaacatc acgctgacgc atctgcatgt
     1381 cagtaattgc cgcgttcgcc agcttcagtt ctctggcatt tttgtcgcgc tgggctttgt
     1441 aggtaatggc gttatcacgg taatgattaa cagcccatga caggcagacg atgatgcaga
     1501 taaccagagc ggagataatc gcggttactc tgttcattgc tgaccccaca aacagatttc
     1561 acgctcaatc tcacgacgag tcatgagacc tttccattgc ttaccgccag catatgtcca
     1621 gcgacgtagc tgatcacatg cgcctttgat atcgccctgg tttattttgc gaagaagcgt
     1681 cgatgttctg aaattgccag cacccacgtt gtaaacgaat gagtaaagag cgccgcgcat
     1741 tgtttccggt atatcgactt tgatgtacgg gttaatttgt ctggcgaccg tggcaaggtc
     1801 tttattcagg agggctttgc attctgcttt ggtatacgtt ttaccgagca tgatgtcttt
     1861 tcctgtatgc ccgtgacata cagtccatac accaacaata tctttgtatg gtatgtagct
     1921 gacaccttcc agaccatcgt taccacttgg gccagtgatt aacactgatg ctatagcaat
     1981 tgctccgcca ccaatagcag cagcaacggc ttttcgtaat gatggaggca ttattcacct
     2041 ctcgcagcct tgcgcttatc ttctttaatc ttgaaataaa ggtttgtcag gtacgtcagc
     2101 aggccaaata ccaggctacc cagcacacct attgctgccc actgtgaggg cgtgacttta
     2161 tcgagcagct gtaaaaacca gtaaccggca ctacctgctg aggtgccata ggcgacaccc
     2221 gttgttaact tatccatgga tttcataacc ccacctcgca gacaaagcgg gtgtaaattg
     2281 agggaataca acgtatcgca aaaaagcaga aacgtaacag actcggagtc agtgaataac
     2341 tcaggtattg ggttatcagc taatatcgag actcaaaaaa tggaaaaacc cgctcgacgg
     2401 cgggtttaag ctgtgtgacg aagtaaccac tcttaacagc ataaccaatt ttttacgtac
     2461 gtaaaccaca aaatgatatt tgcgagaatg ctaccgagta ttgaaaacac cactacaaat
     2521 acataagcaa atctcaacaa ataaccaaca aataatttcc ggtgttattt ttagccgatt
     2581 taaattgaac cttcaaatta caga
//
LOCUS       DAZKJR010000290         2584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKJR010000290 DAZKJR010000000
VERSION     DAZKJR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.292"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1029
                     /locus_tag="ACHOWS_005178"
     CDS             <1..1029
                     /locus_tag="ACHOWS_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFQ0050596.1"
                     /translation="IYYLFRQMSLCVLIFLALVNKVSENTKQRNLFSKKVTLCITLFF
                     VLGGPIVAHIFSSHYESYDLHIAELTNKNGQVVWKASYVTIMIFMWLTLLSVNLYFNG
                     LRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFS
                     ALRVTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKKPYCVMIMDIDHFKKVNDTWGH
                     PVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERL
                     TGDNPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEI
                     LINRKDDE"
     gene            1306..2514
                     /locus_tag="ACHOWS_005179"
     CDS             1306..2514
                     /locus_tag="ACHOWS_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0050597.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYVFPQLGAKTLDTITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
BASE COUNT          730 a          493 c          648 g          713 t
ORIGIN      
        1 atttattatt tgtttcgtca gatgagtttg tgcgtattaa tatttctggc attggtaaat
       61 aaagttagtg aaaacacaaa acagcgcaat ttgttttcca aaaaagtgac tttgtgcata
      121 actttgtttt ttgttcttgg ggggccgata gttgctcaca tattttctag ccattatgag
      181 agctacgatt tacacattgc cgagttaacc aataaaaatg gtcaggtggt ttggaaagcc
      241 tcatatgtta ccataatgat tttcatgtgg ttaaccttgc tttcggttaa tctatacttt
      301 aatggtttac gctgtgatat ctggaacggg gtgtcggtga ttgctttttg tgccgtgctg
      361 tacaatatct ctctgttatt tatgagcagg tatagcgtgt ccatttggta tattagtcgc
      421 actattgaag tagtcagtaa gttaaccgtt ttggtaattt ttatgtgcca tatattcagt
      481 gcgctacgag taacaaagga cattgcacat cgcgatccct taaccaatat atttaacaga
      541 aattattttt ttaatgaact gacggttcaa tcagcatcgg ccaaaaaaaa accttattgc
      601 gtcatgatta tggatattga ccacttcaaa aaagtcaacg acacctgggg acatccggtt
      661 ggcgatcaag tgataaagac agtggtgaat atcattggga aaagcatacg accagatgac
      721 cttttagcgc gcgttggcgg cgaagagttt ggcgtcttgc tgacggagat cgatactgaa
      781 tgtgcgaaag ctttagcgga aaggattcgg gaaaatgttg aacgtttaac tggcgataat
      841 cctgaatacg ccataccaca aaaagtgaca attagtattg gcgctgttgt aacgcagaaa
      901 aatgagttaa accctaagga gatctatcaa ctggctgata acgcacttta cgaggcgaaa
      961 gagaccgggc gtaataaggt ggtagtaaaa gaggcagaaa ttcttatcaa ccgcaaggat
     1021 gatgaatgag tctgtcgcta gttgtcgacc tccgcctggg gcttctcatc ccccagtgtg
     1081 tgaaatatac gaaaaaaagc tcgcactttc gtacgagctc ttctttaaat atggcggtga
     1141 gggggaccgc ctgaaataaa tctgacttaa tccgggagtg ttatataact tcattataaa
     1201 caatgcatta caatttttgc tgtccgatgt tatctaatgt attcttagtg aagtgagaga
     1261 ctaatggcat actggatggc ataccagagt aacaggaatt agcgtatggc agtattgacg
     1321 gatactaaag caaggcatat caaacctgat gacaaaccat tgccccatgg gggaattaca
     1381 ggactgactc ttcatccttc ttcagtaaag gggcggggga aatgggtttt tcgttatgta
     1441 agtccggtga cacaaaaaag gcgtaatgct ggattgggta cttaccctga ggtcagtatt
     1501 gctgaagctg cacgtactgc ccggataatg cgagagcaac ttgctgcagg tgatgatcct
     1561 ctggagatta aaaaggctga agctgaaaaa gttgttatcc caacatttgc cgatgcagcc
     1621 cggcgtgtac atgcagaatt gtcaccggga tgggaaaacc caaagcatgt caggcagtgg
     1681 ttgtcgacgc ttgaaaatta cgtgtttcct caactgggag caaaaacgct ggatacgatt
     1741 acggctgcgg acgtggcaga gacactgcgt ccagtctggt tgaccttatc agaaacggca
     1801 agccgggtta aacagcgcat tcatgttgtt atgcagtggg gctgggcgca tggtttttgt
     1861 gtggcgaatc ctgttgatgt ggttgatcat ttgcttccac agcaatcaag aggacgtgat
     1921 gaacaccagc cggcaatgcc ctggaggcag ttaccgcttt ttgtggcgac cagtgtgtat
     1981 acagatgaac cttataatgt tacccgggca ctgttattaa tggtgatact gacagcaacc
     2041 cgctcgggcg aagcaagggg aatgcgctgg gctgaaattg attttcataa gcggatatgg
     2101 acgatacccg cagaaagaat gaaagccagg atacagcatc gtgttccttt atcccgacag
     2161 gccattcacg ttctggaaaa tatacgtggt ctgcatgacg aactggtgtt tccttctccc
     2221 agaaagcagc agatcctttc agatatggtg ttgacgagtt ttctacgtaa aaagaaggcc
     2281 atcagtgata tacccggacg agtggctaca gcacatggtt ttcgttcaac attcagggac
     2341 tggtgtagcg aacagggata ttcgcgggat ttggcggaaa gggcgcttgc ccacacgctg
     2401 aaaaataagg ttgaggcggc atatcaccgg actgatctgc tggatcagcg tataccgatg
     2461 atgcaggcat gggcggatta tgtgatgtct cagattatgg aaaaccagcg atgaaatcac
     2521 atctcacaga tttgcagatg tcgtctgtaa tcacgaaaaa tatacttatt gagttataat
     2581 gctt
//
LOCUS       DAZKJR010000291         2562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKJR010000291 DAZKJR010000000
VERSION     DAZKJR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.293"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..194
                     /gene="hemP"
                     /locus_tag="ACHOWS_005180"
     CDS             3..194
                     /gene="hemP"
                     /locus_tag="ACHOWS_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFQ0050598.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(198..1634)
                     /gene="selO"
                     /locus_tag="ACHOWS_005181"
     CDS             complement(198..1634)
                     /gene="selO"
                     /locus_tag="ACHOWS_005181"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFQ0050599.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVALSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYASRPPDWGKRLEVSCSS"
     gene            complement(1697..2410)
                     /gene="ydiV"
                     /locus_tag="ACHOWS_005182"
     CDS             complement(1697..2410)
                     /gene="ydiV"
                     /locus_tag="ACHOWS_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFQ0050600.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
BASE COUNT          696 a          659 c          587 g          620 t
ORIGIN      
        1 ttatgcgtta tacggatagc agaaaactca cgcctgaaac ggatgccaat cacaagaccg
       61 cttccccgca gcccatccgg cgaattacca gccagacact gttaggtccg gatggcaaac
      121 tgattatcga tcatgacggg caagaatatc tgctccgtaa aactcaggct ggcaagctgc
      181 tgttgaccaa gtaaccttta actcgagcag ctgacttcca gccgtttacc ccagtcaggt
      241 ggacgactgg cgtagtcatc atctctgtcg ctgaaaggat ttcgcaacgc ctcatgcagg
      301 cggtgcaatt ccgtcatatc acccttttct gccgcttcaa tcgcccgttg cgccaaccag
      361 ttgcgcaaca ccagagctgg gttaacgctt tgcatcagtt gctgacgctc aatatcgcta
      421 acctcgtctt gttgcaaacg cctccgataa cgggcaaacc agtcatcaaa tgccgcacga
      481 tcaataaact catcacgtag cggtgacgcc gcgctgtgct gctcggtcag actcagcatg
      541 cgaaatgtgc gggtataatc gctgcgctct cgcgccatca gactgaataa ttcatttagt
      601 agcgcgttat cctctttttg ctccgtcatg aagcccagtt tctgccgcat ccgttgtcca
      661 taatgcgtca acaaaacctg ctgatagctg tccagggcct cattcagggc atcaacggcg
      721 acaaatggcg acaatgtctg cgccagacgc tgtaaattcc acaacgcgac ggcaggttga
      781 ttatcaaagc tgtaacgccc ttggtgatcc gagtgattac aaataaaacc gggttcgtaa
      841 tcatcaagaa aaccaaacgg cccgtaatca agcgtcagcc ccagcagcga catgttgtcg
      901 gtattcatca ccccatgagc aaagccgacc gtctgccatt gggcaattaa cgatgcggta
      961 cgtgcgacaa catcgctaaa ccagagacgg tatttgtcct catcatcttc aagatgagac
     1021 cagtaatgac gaatggcaaa gtcagccaac tgacgaacct tttccggctc gcggcgatag
     1081 taaaaatgtt cgaaatgacc aaagcgcaga tgacttagtg ccacacgcat cagcatcgcg
     1141 cctggctcca ccgtttcccg atacactggc gaatcgctgg tgacgatact taacgcgcgg
     1201 gtcgtcggaa tgcccagata atgcatcgcc tcactggcga gactttctcg tatcgtcgaa
     1261 cgtaaaaccg cccgtccatc acccattcgc gaataaggcg tcaggccagc acctttcaga
     1321 tgccagtcca ttgtagtgcc atcagcaagc cgttgttcgc cgagtaaaat gccgcgccca
     1381 tcacccagtt ggcccgccca gacgccgaac tgatgaccac tgtaaacctg cgccagtggt
     1441 gacataccag gcagtagggt ttcgccgccc cagacacctg cgccattttt aaacagcgac
     1501 gatggaatac tcagcgtgtt agcgagttcg gtattatgcc aaatcagccg ggcattattt
     1561 aaaggcgtag gggaaagtgc tgtataggtt tctggcaatt catcgcgcca gcgggtaaca
     1621 aaagacaggg tcatagatcc tcctatttga tagtgtagac ggttactctc gtcttaaaca
     1681 ccagcaaaca ggagggttat cgctgaacca acgtcgttat ctgatttaca ggaacggcag
     1741 gccacaggca tccctggaga gcatggaaat caaagggggt aatctgtgcc attatttctg
     1801 gcgtatcaat gccccccgcg atgatgcaat tgcaacatgg ggaaatttga gcctggatcg
     1861 cacgaataaa cggttcgaag gagcgatgcg tgatttgttg ctgaataaaa cttttatcca
     1921 gcatgacgcg ggtgaaaaga ccatcaaaaa ccgctttcat tgtgctattg cctgcgccca
     1981 aattacctaa cacaagggga tacatttgcg ataaggacaa aagatcgcga ttatcttttc
     2041 cttcatcgag atgcgggtaa ttctcattaa tcaacagttc aataaacgga tattttaata
     2101 gttcgcctgc gtaattatcc ctgtctaata ataatgtagc cacttgtggc gttaaattaa
     2161 gccaggcaaa gagtttatgt tgaataaaaa agtgttggca tgattttagt aattccaact
     2221 gctctgaaaa taattgccaa tgttgctcct cggtaagttg cgctataacc cgactcgtcg
     2281 ggatacgaac cgtaccatct tcactggaga aatgggtaat taactcaacg ccgacaaggt
     2341 cttgctggtt atctcttatc ggcaggaaat agcaatcaga atgataaaga ttctccaaaa
     2401 aaatcttcat ggtaaccgtc cctctcgaag gatgtttttc agtatccggc taaaagggat
     2461 gaggccataa gacataacaa ttacagaagg agtaactttc atttgttcca tgttaaccac
     2521 tttttcaggg gtcctttttt tagattatcc tgattataaa cg
//
LOCUS       DAZKJR010000292         2555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKJR010000292 DAZKJR010000000
VERSION     DAZKJR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.294"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(96..314)
                     /gene="ymgF"
                     /locus_tag="ACHOWS_005183"
     CDS             complement(96..314)
                     /gene="ymgF"
                     /locus_tag="ACHOWS_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFQ0050601.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQH"
     gene            complement(446..1969)
                     /locus_tag="ACHOWS_005184"
     CDS             complement(446..1969)
                     /locus_tag="ACHOWS_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFQ0050602.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIREALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2305..2553)
                     /gene="ymgC"
                     /locus_tag="ACHOWS_005185"
     CDS             complement(2305..2553)
                     /gene="ymgC"
                     /locus_tag="ACHOWS_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFQ0050603.1"
                     /translation="MNNSIPERFILQCALFKNLEREVFMTHGYVDSYIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
BASE COUNT          772 a          570 c          528 g          685 t
ORIGIN      
        1 acaactattt gcgcaataat tcctttcttc aatgatccag gcgttcactg ccagcaaccc
       61 attcagccgc atactgacag tgagaaactg aaagattagt gctgatccct gtggcttacg
      121 agtctttccc ttaatcgatt aatcagattg atcgccagcc aaacaagggc attaagcaaa
      181 tttatcgtca caagcacttc tcccagcccg gtactgggtt ttgcaataag gatgaccagg
      241 agagtgatca gcataaatgc aatggaaaag atgatataga gagtgaaata atccagatta
      301 ttactattgt tcatgctact aatggcaatc ctgtcaaaac tttcaaacaa ttatagcaaa
      361 caaaataatg aacaatagta acaagaggca acgagctaat tagctgtcta taattacacc
      421 gcactatggc cttactgatc gattttcact caaccacaac cttcaccttc ggtttagaaa
      481 ggataatttt gaccaggtcg atgtatgtaa caggtttata gaaataataa ccctgctgaa
      541 atgtgatatt attttgattc agatagtcaa gttgttcttt cgtttcgaca ccttcagcga
      601 cgatactgat cgaaagttta cgcgccagat ccaatacaca atccagaatt cgggttgatt
      661 ctgggtctgc gttgacgcgg ccaacgaaac tgtgatcaat tttgatataa tcaatatgca
      721 ggtcatgcag ataagaaagc cctgagtagc cagtaccgaa atcatccagc gcgatgacaa
      781 aaccattttc atgcagaatg ttcaaccgct gaaccagact ttcatcaaca tttaatggct
      841 cacgctcggt aacttccaga acgaggttta aatcgcggcg ggtaaaactg tcacggtaat
      901 ttaagcactc gtcgacaaac gtcggcgaaa taatatgcga ggcgctaaaa ttaattccaa
      961 tatgaaaacc ttccggcaat ttactcgcga tagcgttcat ctgtctggca acctgattca
     1021 tcaggcttcg cgtaagcgga acgattaatc cggatttttc agcaagtgga ataaatgccg
     1081 caggtgatat atatccaccg tgaggttgtt tccagcgggc taacacctca actccccgca
     1141 atgtcccttc ccgaccattt accacaggtt ggtaaaaagg gacgatctcc ccattattta
     1201 tagcccgtcg caggatctct tcaggcgttg cgcttttatt gaaataccta tcaagcagga
     1261 aggcagcggc gcaggcgatt aacagaataa atatcaaaat cccaaagcct ttatcgataa
     1321 gtctgtagaa gctaaagagt ggcggcgggt taaagataat agtgaaatga tattttgtag
     1381 agtgaacctt ctcggtatag ggatagtgtc cattaagcgt catcacatca cccgtaggtc
     1441 caataatgtc atctgccacg cgtaatacat accttacccc tttcaagggt acattaagcg
     1501 cctcgcgaat atgctgatca cttatcgtaa ccaaaatgcg cgtatctgca aattggctct
     1561 gatagagcag gataggtaat ctattaacgg tgtcgattgc cggagccagc agtaaattac
     1621 tgtccgggaa aacaggaata cgagacagta ggacccgatt ccccggtaag gatgtacacc
     1681 agaccgttcc ctgtttgaga atgatgattg tgcgcaggtg aggcttcaga gctgcttcag
     1741 tgccaagctg atattgctcc tcgaggtcgc attccttccc ggcaatgtta accgccgttt
     1801 gagtcgcctg tgacgcttct tcaagtatat gattgatatt gttggcggca tatctcgctc
     1861 ccgccaggta ctctgctttc gcggaatacc agagctggat gttcaaaata gcgacaccgg
     1921 ttataaacaa gcatatcgca acgaggatgg gtatgagtgt attgcgcatt cgcttgtccc
     1981 ggacaaaggg tttaatacat gaaaatgacg ttcattcgct cgcgctggcg tctacagaga
     2041 gcatggtaga gagcatcaca aatatccacg ccgaagataa aattttctcc aacttcatct
     2101 aacctattaa ttattgagca caatgtaggc agaattaaac aaattaggtg ggatatcagg
     2161 cgtcaagaat gaaaaacgag cgctctccat tcctgtttgc cccctgatat tgcggatata
     2221 ttaagaaagc ataacgcctg aaatgctcac tttgcaccag catggtgata cagctgatgt
     2281 ttattctata accttactca agttctaaga gatcacggat tccctgtcat taataatgat
     2341 atccgttagt gtagtcttat gcatttcaat gtactgcaat atttgcagat agagatcaga
     2401 aagaatcaca ctggtttcat cttttaaacg caagcgtaaa gcctgatcaa taatataact
     2461 atcaacatac ccatgcgtca tgaacacctc cctctcgaga tttttaaaca aagcacattg
     2521 caaaataaat ctctctggga ttgaattatt catgt
//
LOCUS       DAZKJR010000293         2543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKJR010000293 DAZKJR010000000
VERSION     DAZKJR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.295"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            350..493
                     /locus_tag="ACHOWS_005186"
     CDS             350..493
                     /locus_tag="ACHOWS_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFQ0050604.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            506..715
                     /gene="cspE"
                     /locus_tag="ACHOWS_005187"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             506..715
                     /gene="cspE"
                     /locus_tag="ACHOWS_005187"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFQ0050605.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            881..1690
                     /gene="rlmA"
                     /locus_tag="ACHOWS_005188"
     CDS             881..1690
                     /gene="rlmA"
                     /locus_tag="ACHOWS_005188"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFQ0050606.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1687..2253)
                     /gene="mntP"
                     /locus_tag="ACHOWS_005189"
     CDS             complement(1687..2253)
                     /gene="mntP"
                     /locus_tag="ACHOWS_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFQ0050607.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDKEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     regulatory      complement(2385..2475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          673 a          595 c          620 g          655 t
ORIGIN      
        1 tggtggccgt taactgactg ttttatgaga aaaagtgata taacttttta ttcattgcat
       61 agcagaaaat gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac
      121 tgaacttatt gaactatgtt agaaaatacg tcagtttaag tatctgcctg aactggcaag
      181 gttaagcaca atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa
      241 gacgtatcga gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa
      301 cgttgtatgt gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat
      361 tttcagtaaa gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc
      421 ttatattggt gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa
      481 gaaaacaatc taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa
      541 cgagtctaaa ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca
      601 cttctctgct atccagggta atggtttcaa aactctggct gaaggtcaga acgttgagtt
      661 cgaaattcag gacggtcaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa
      721 tccactgatc tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag
      781 gctttttact ttgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc
      841 gcgttacact tgcggccttt agtatcctgc cggagttgtc atgtcgtttt cctgtccact
      901 ttgccatcag cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt
      961 tgatatggcg aaagaagggt atgtcaatct gctgcccgtt cagcataaac ggtctcgtga
     1021 tccgggcgac agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta
     1081 tcagccgctg cgtgatgcaa ttgtcgccca actgcgggaa cggcttgatg aaaaggccac
     1141 agcggtgctg gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgct
     1201 gcccgaaatc accacgtttg gcctggatgt ctcgaaggta gcgataaaag cggcggcgaa
     1261 acgctatccg caggtcactt tttgtgttgc ttccagccac cgtttgccgt tttccgatac
     1321 tagtatggac gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcgcgagt
     1381 agtgaagcct ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct
     1441 gaaggggctg atttacaatg aagtacatct tcatgcacca catgcagaac aactggaagg
     1501 ttttacatta cagcagagtg atgagttgtg ttatccgatg cgtcttcgcg gtgatgaagc
     1561 cgtcgcatta ttgcagatga cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac
     1621 actggcagca aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg
     1681 ttcttattaa ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagcaccag
     1741 cccgccgaga atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat
     1801 ccctaatgtc gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt
     1861 gacctgcagg aaggcgagac caacacccac agccatggca tccaggctgg tggcaatcgc
     1921 ggtggttacc agtagccaga aaccgtgccg acggcgcggc tctttatctt catcatctgc
     1981 gccacgaaaa ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat
     2041 ccagtgattc cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag
     2101 cggtgtcagg gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt
     2161 cggtttatgg agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc
     2221 aaacgcaaga agaacagtag cagtgatatt catgacaacg tcctgaccgg ggtatccata
     2281 aaacacatca tcgcccccag caaacatgca tacatgaaca acaagccgcg atgggttttc
     2341 gcgttcatgc aggcatagcg attgatgtgc atatggtctc gcctgactga aagtgttcag
     2401 tccgtacgtg ccacgttttg caacgagtat gttgacacgt acatttccgg aatctggaaa
     2461 tcggctactc cccaaatgag ggcgcaacct taacatattt tgagaatata aaacaactgt
     2521 tggaatatat tatttcattg ctt
//
LOCUS       DAZKJR010000294         2512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKJR010000294 DAZKJR010000000
VERSION     DAZKJR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.296"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..270
                     /locus_tag="ACHOWS_005190"
     CDS             <1..270
                     /locus_tag="ACHOWS_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFQ0050608.1"
                     /translation="PPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALGV
                     QLHESAVVSVRYGARVRLADGRTAYWNDSTRRIDITPAQNTEAPQ"
     gene            267..671
                     /locus_tag="ACHOWS_005191"
     CDS             267..671
                     /locus_tag="ACHOWS_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050609.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATRPQPGDELEITIV
                     SPDRTTTAIITDILNGRPDNTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNTELTRTLASINRKAAIARYVHSAANRQSRR"
     gene            720..890
                     /locus_tag="ACHOWS_005192"
     CDS             720..890
                     /locus_tag="ACHOWS_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050610.1"
                     /translation="MNDTRRISDRELTKMIESAQQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            887..1213
                     /locus_tag="ACHOWS_005193"
     CDS             887..1213
                     /locus_tag="ACHOWS_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0050611.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTNKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
     gene            complement(1279..1542)
                     /locus_tag="ACHOWS_005194"
     CDS             complement(1279..1542)
                     /locus_tag="ACHOWS_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFQ0050612.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(1580..>2512)
                     /locus_tag="ACHOWS_005195"
     CDS             complement(1580..>2512)
                     /locus_tag="ACHOWS_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0050613.1"
                     /translation="HNIWYDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAG
                     GYTGGGLTTDQMEQAVFDYLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVV
                     LQEGPALVYTPEDIIFLRMYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAH
                     MGFLLYTNDPGITTEMEDEIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSA
                     KDEFSNIKGISAQDVFTAHRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKI
                     MNAVNSDPDIPEHLYLCFDVDAGHVAEEKAVPVG"
BASE COUNT          639 a          736 c          682 g          455 t
ORIGIN      
        1 ccacctgaca cggaaaccgt gaaaaacggc ggcatcagtg cgcccgctgg cggcgttcgc
       61 gtgagtgtcg ctctgaccgc tgagccacac atcaccgcac aaatcacccg cgagcttgat
      121 gcgctgggcg tgcagctgca tgaaagcgcc gtcgtttccg tccggtacgg tgcccgcgtg
      181 cgcctggctg acgggcgcac cgcgtactgg aatgacagca cccgccgcat tgatatcacc
      241 cccgcacaga atacggaggc gccacaatga cccgctttta ttgcgccatg cagcgacagc
      301 gttttgacga aatgatgcgc cagcgcacgc ccttactgac cctgaacaac attgccacga
      361 gaccgcaacc aggcgatgag ctggaaatca cgatcgtcag tcccgatcgc accaccacgg
      421 ccatcatcac cgacatactc aacggacgac cggacaacac taaccgcagc tggcttgtcg
      481 ctgttcgtcc gcgccgtccg cccggagagc cggagccgct cacgcctgcc ggaaaaatca
      541 tgcggcagta ccacgaagag caggcacaca aacacaacac agaactgacc agaacactgg
      601 catcgattaa ccgcaaagcg gcaattgccc gctatgtgca ttcggccgcc aacaggcaat
      661 cacgcaggta aacaaagacc atgaacatga ccagaaacag ccaactaaag gagcgcacaa
      721 tgaacgacac caggaggatc agcgacaggg agcttacgaa aatgattgag tcggcacagc
      781 agctgctttc acgacaaaac ccggtcgtgg aagaaacatg gtgggagaat ttactggcag
      841 cactgaccga actccgggaa cgccggaaca acgaaggagc cgccaaatga agaccccagg
      901 aatgaccgga aaggaaatca ttttacgcta cctggaaacg cacgcacgtt tcagcgcccc
      961 agaactggta gaaaaactgg gcgtgaaaag aaccgtcatt aatcaggccg ccggaaaaat
     1021 ggtacgggaa gggttgctgg ttgtggacgg gtacacgaac aagataacgc attaccggaa
     1081 gccaacggaa aaggaacgac aggaactggc gcaacgggct gaacggcagc aggaaaaatc
     1141 agtcatcgaa gcctgcaagc gcagcgaaat catgaagcga attctgttta tctacggcgc
     1201 tgcattccgt taaaggagct ggtgagtcgg ggcaaaaatt ctttttgccc cggcgaagca
     1261 gcagacaagc gaagcgcgtc aggaacgccg cgacagtagt tcaagcgcat accggcgatc
     1321 ttccgggcgc atggcatcca ccaggaactt caccatattg cgaccggagc gggcaccggg
     1381 gctgagggta tgggaaaagg tcatcgtaca gacgaaggtg tgcccgcatt cgacatcact
     1441 gcatgcacag tacagatccg caagttcagg ggttttccag tctgtcttcc ggatgacagc
     1501 aggtgcgcca cagtcaggac accgggcttt aaaaatccgc atcttacctc ccgataacac
     1561 gtattccggg gaggacattt tacccgaccg gcaccgcttt ttcttccgcc acatgcccgg
     1621 catccacatc aaaacaaaga taaaggtgtt ccggtatatc gggatcgctg ttcacggcgt
     1681 tcatgatttt acgctgaagc ggtatcacct catccttgcg gtacgtttca cgcgtcgttt
     1741 ccgggttagg gatggtggcc ccgttcgtgg ggataatccc cgcaagaccg gcaggaaagc
     1801 ggtgtgccgt aaacacatcc tgggcgctga tccccttgat gtttgaaaat tcatccttgg
     1861 cgctcacctc accaatcgga ataattttca ccccatccgg cgaacctttg gggatgttga
     1921 tgtacagatt gcggaaattt cccagcccct tagactccgc caccttctcg cggatctcat
     1981 cctccatctc cgtggtgatc ccagggtcgt tcgtatacag caaaaagccc atatgcgccc
     2041 cgttgttgta gtaacggcga cgaaaaaggc tggcctcgct gttcagcagc gccgagtgga
     2101 tgccaccgat ataatccggc agcccgtaaa tttgctgacg cggatcatac atccgcagaa
     2161 agataatatc ttccggcgta tagaccagcg cgggaccttc ctgaagcacc acgaactcac
     2221 cggttttgcg gcgccggaga tacagcgacg gaagcggctc aagtgccacg acgtcgccga
     2281 atacgttacg caccttgagc agggcgagat cgccgaacaa caggtagtca aataccgcct
     2341 gttccatctg gtccgttgtg agaccaccac cggtatagcc ccccgccacc atgttacggc
     2401 gggcatacag caccccaccg tgctgggcgt taaggttcgg gagctgcgcc agtgccaggc
     2461 ggttaatcgg cagctgccag tggttgtgtt cgttgtcata ccagatgtta tg
//
LOCUS       DAZKJR010000295         2473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKJR010000295 DAZKJR010000000
VERSION     DAZKJR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.297"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..546
                     /locus_tag="ACHOWS_005196"
     CDS             <1..546
                     /locus_tag="ACHOWS_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFQ0050614.1"
                     /translation="QFELNELGSGIHLVIKNCSICKKMVPFYSDDNCLLCNKKNEELS
                     PTRDEINKIREFDVTISQDNLNSVTQRLLPTPLAAVRARRVAFENPYRNKVLSESLPA
                     GNLLYRSALAFIQALIALVPSKKILDLDKITDALRRRYVHLEQLEYAELRNALACALS
                     TCYNRKYLIKVAKGKYLPVDD"
     gene            628..975
                     /locus_tag="ACHOWS_005197"
     CDS             628..975
                     /locus_tag="ACHOWS_005197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0050615.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            1148..1312
                     /locus_tag="ACHOWS_005198"
     CDS             1148..1312
                     /locus_tag="ACHOWS_005198"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0050616.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(1316..1783)
                     /locus_tag="ACHOWS_005199"
                     /pseudo
     CDS             complement(1316..1783)
                     /locus_tag="ACHOWS_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1847..2131
                     /locus_tag="ACHOWS_005200"
     CDS             1847..2131
                     /locus_tag="ACHOWS_005200"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0050617.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQPRVSVARIARKHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPAFYAR"
     gene            complement(2348..>2473)
                     /locus_tag="ACHOWS_005201"
     CDS             complement(2348..>2473)
                     /locus_tag="ACHOWS_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0050618.1"
                     /translation="LLITFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT          737 a          535 c          562 g          639 t
ORIGIN      
        1 caatttgaat taaatgaatt aggctcaggg atacatctgg taataaaaaa ttgtagcata
       61 tgtaaaaaga tggtaccttt ttattctgat gataattgtt tgctttgtaa taagaagaat
      121 gaagagttgt caccaaccag agatgaaatt aataaaattc gcgagtttga tgtaacaata
      181 tcccaggaca atcttaattc agttactcaa cgattgttac ccacaccatt agctgcggta
      241 agagcccgaa gagttgcatt tgaaaatcca tatagaaata aagtactaag tgaatcatta
      301 cctgctggaa accttcttta cagaagtgca ttagctttta tacaggcctt gattgcatta
      361 gtcccatcaa agaaaatact ggacttggat aagataacag atgctctgcg taggcggtat
      421 gttcatcttg agcaattaga atatgccgaa ttaagaaatg ctttagcttg tgcattgtca
      481 acttgttata atagaaaata tctcattaaa gttgcaaaag gaaaatattt acctgttgac
      541 gactaggtgc gtattaccgt gaaggagatc ggtgagtaac cccctgtgtc agcatagttg
      601 tcgcctcaat tttttaagga gataacaatg ccaggacaac caataccaag tgagctcaaa
      661 gagaaaggac tgatatggct tttaccacca tactgctggt cacatcgtca gatagcccgt
      721 aagctaaaga tcagtgcctc agttgtatca aaatggagat atgagttagt ggagcaaggg
      781 cttttacctg aaaatgaaaa gattacctcc gttgatgttg aagactggac accggagcgt
      841 cgattctcag ttgttcttga gacggctacg ctgtcagaaa tggagcttgc tgattattac
      901 cgacgcatgg gattgtttgt tgaacaggtt aaagaatggc gggctatctc aattcaggcg
      961 catgaaaaaa attgacggaa aatattcgaa cagacagggc attgcgtgac gcaaaaaata
     1021 agttacgaga actgtagaaa gagcttaagc gtaaagataa agctctggcg gaagctgcgg
     1081 ccctgcttat attgagggaa aagttcaatg ccctctggga caacagcgag gaagactgac
     1141 ccctctcctg gagcggcgta aaattattgc ccttgtccgc gaggccgtct ccaacggcgc
     1201 ccggaaaagt caggcttgta ggtgtatcgg tatttcaatt cggacacttc agcgctggac
     1261 tgatggcaac gttattacca cggatgtaag cgtcaacgga gcaccgtatt gacgcttatt
     1321 tattggtgag tactacgttc catggcagga gttcatcaac tcggttggaa ggccattccg
     1381 gcagtacgct caggataagg cgcagatacg cttccggatc gataccgttc agacggcagg
     1441 tgccgatcag cccgtacaac agtgctccac gctcgccgcc gtgatcgctg ccaaagaaca
     1501 taaagttttt ctttccgaga cagactgcac gaagcgctct ttccgcagca ttattatccg
     1561 cctccgccag accgtcatca ctgtaataac agagggcatc ccactgattc agtacatagc
     1621 tgaacgcttc gcccagtctg gattttttcg acagcgtacc attcttctcc accatccatt
     1681 catgcagcga cgtcagtaac actttgcttc gctgctgcct gacggaaaga cgctctgact
     1741 ccggtaatcc ccgtatttcg taagcgtaaa ccgaccgccg tatgtagcca ttaagccagt
     1801 attggtaacg taggtgccca ccttttcaaa ctagtggacg cctgttatgg agcaaaaaac
     1861 attatccgca gaaccccgca gatcattttc aaataatttt aaacttcaaa tggttaaact
     1921 ggctttacaa ccaagggttt ctgttgcccg tattgcccgg aagcatgata tcaatgataa
     1981 tcttctgttc aaatggctca ggctctggct gatcaaaggg cgtatatcac ggcgtcttcc
     2041 ggtaaggaac tcttctggca ttggcgttga attattacct gtggagatga cgctggatga
     2101 gcggccttac cccgcttttt acgcgaggtg attatgaaga tcaatgacta gccgtcgaac
     2161 cgtgtgcatg aactgctgca ctgaaacttc tcagctgtaa aataatcctt acgctacgta
     2221 cttcccggag cgcttacgat taatcggatg cagtcctgac tggaagaaag aaaaatggga
     2281 aaaaataaga ggaattaata tcctccacgt tcctgaaatc ctggaggata ttaatgtcat
     2341 caaaatatta gcgaacgata atccctaaca aaatacccag tgcgccaaag tcagcatcac
     2401 tgaaggtggt gttagcaaaa ccaatgttac ccagcacagg caataagaat actggcagga
     2461 aggtaataag caa
//
LOCUS       DAZKJR010000296         2418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKJR010000296 DAZKJR010000000
VERSION     DAZKJR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.298"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1076
                     /locus_tag="ACHOWS_005202"
     CDS             <1..1076
                     /locus_tag="ACHOWS_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFQ0050619.1"
                     /translation="SPSIRPSLYAYGANATTLKPFSVALGAGSVADRGAGIEAYMPET
                     KRWNPGDVVKSRMSDKQLNRLMEIDKLISAYNSQETLSAEETKQLEEVKKERADMFAT
                     YIATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQTVTEKADAAL
                     DKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEHKLLISENSK
                     QISTNTLGIRENTQRVQDEGQARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKE
                     DIDKNRKRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSA
                     VKVNAAWDTENGAGVSAGMALGW"
     gene            complement(1506..2081)
                     /gene="dolP"
                     /locus_tag="ACHOWS_005203"
     CDS             complement(1506..2081)
                     /gene="dolP"
                     /locus_tag="ACHOWS_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFQ0050620.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
BASE COUNT          695 a          567 c          592 g          564 t
ORIGIN      
        1 catccccgtc aatacggccc tcgctgtacg cttacggtgc aaacgcaaca acattgaaac
       61 cattctctgt cgctctgggc gcaggaagtg ttgctgatcg tggcgctggt attgaagcat
      121 atatgccaga gacaaagcgc tggaatccag gggatgtagt aaaatccagg atgtctgaca
      181 agcagcttaa caggcttatg gaaatcgata agctaatatc cgcttataat tcacaagaga
      241 cgctgtctgc tgaagagact aaacaactgg aggaagtgaa aaaagagcgg gctgatatgt
      301 ttgcgacata tatagcaacg cacgatgctg tatctgtagg tgttggtgca acgacaacag
      361 gacgacatgc tgttacaaga cagataacta gcgttgcagc aggttctgtc gatacggatg
      421 ctgttaacgt tgcacagctc agggatttgc agactgtgac agaaaaggca gatgcagcgc
      481 ttgataaacg cataacaaac aacaccagtg cgataactaa aaatctgcag gtggttaaca
      541 gtaataaaga ggaaatcagt aacattaata aaacgacaaa aattctggat gaaagagtga
      601 cagccaatac acagacagca gaagaacata agctgcttat ttcggagaac agcaaacaaa
      661 tcagtaccaa cactcttggt atcagagaga atacacaacg ggtccaggat gaaggacagg
      721 ctcgaatccg gggagacagg gaaacactgc gtgcagcgac cggttatacc gacatgcggg
      781 taaacgggct ggagcgtaac acgaataaac gttttgcaca gcttaaagag gatatagaca
      841 aaaacagaaa acgtgcagat gcaggtattg caggtgcgat ggcaatgacg gggataccgt
      901 ccgtccccgg gaaaaaggtc tcatttggta tggccatggc cggataccgg agtgaaggtg
      961 ctgttgctgc cggcttccat tttaatacaa gtgaaaacag tgctgttaaa gtgaatgcag
     1021 cctgggacac tgaaaatgga gctggcgttt cagccgggat ggctctggga tggtaatgct
     1081 tccgggccag aagccgttcg cagatattgt tatccagggg acactgcacc accattcagg
     1141 aagcgattca gtttatcgca tcggctcagg atgtaattaa tacggacaat ctggctcctg
     1201 caccggagcc agacgtacac gtgtcaacgc cacgatcttt gacctgtatg gataaaagct
     1261 cagattattt accagcagcg acatccggag agcaccggga ttttatctgt gagtgaacat
     1321 acccgggcaa aaaaatcccc cgaattcagg ggggtgtcaa agaggaatta tgtatattgg
     1381 ttattataat gagcacgtct ccggtattcc gcgacgaatg ttcacgtggt ggcatgaacg
     1441 gcccggaaac gtgctcttct gatttttaac gtaaatttgc actaaaggca actcagatac
     1501 ggccttcacc gtatacagga gaatgccgtc gtgacccgct tcacgccact caccctactg
     1561 gcaatatctg ctgccgctcg cccttcctgc tctgtaacca ggcccagcaa aaagacttcg
     1621 ctgttttctg tcaccacctt gacactggat gatttgacac gggcacttgt gagtaactga
     1681 gaacgaattt ttgtcgtgag ccaggcatca cctgccatta tgaccggact gacgggagtc
     1741 ccctgccgga tttcattata gacctcctga acaccatcga caccgagtgc tatctgacgg
     1801 gcacgggcag ataattcacg gtccggagac tgaccgatca gcagcacgcg tccctgatat
     1861 gcaatctcac tgatgcgtgc ttcttttctg atttgttcat cttttgaaag tgcactgctg
     1921 accctcagct ccagcacttc atcatccacc tgcgttccga cactgcgtgg gtcggttgct
     1981 gcctttgtcc cgacagcggc tgtacccacc acagttgcag caacgcatcc ctgtaacaat
     2041 aaggctgaaa tcaggacaac agcagaatat aataaacgca tatctccccc ggaatttatc
     2101 gtacaaaaag tttaccttca gtgaaacaaa acaagctacg gactgttatt ctcttccggc
     2161 acagccggaa acagcggata cgttacgtga catatctgta tctgccagac ggaaatgttt
     2221 atgacggata tggatgtact gaaatttaat attgttcatg tttttgcacg aaaacgttga
     2281 tagcgtagta aagattctgt tttttaacta cctggtttgc tgagcaggat ctccttttcg
     2341 ttatacacag gctaaaaggc accgtggccc cccggtgcct ttttcataaa acagagaaaa
     2401 cacggctttg aatatacc
//
LOCUS       DAZKJR010000297         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKJR010000297 DAZKJR010000000
VERSION     DAZKJR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.299"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(125..1138)
                     /gene="argF"
                     /locus_tag="ACHOWS_005204"
     CDS             complement(125..1138)
                     /gene="argF"
                     /locus_tag="ACHOWS_005204"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0050621.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
     gene            1300..1716
                     /gene="rraB"
                     /locus_tag="ACHOWS_005205"
     CDS             1300..1716
                     /gene="rraB"
                     /locus_tag="ACHOWS_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFQ0050622.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1762..2265)
                     /locus_tag="ACHOWS_005206"
     CDS             complement(1762..2265)
                     /locus_tag="ACHOWS_005206"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0050623.1"
                     /translation="MNNIAPQSPVIRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          635 a          579 c          556 g          625 t
ORIGIN      
        1 ctaaatcctc cggaaaattt acgtatagca atagaaaaat ttggctggaa gaaaaaaact
       61 atcactgcat aaaataatgt gatgccggga tgtttttgta tttcccggca tctttataga
      121 gatattaatt atttaattta ctgagcgtcg cgaccatcac cgctttgatg gtatgcatgc
      181 ggttttccgc ctgatcaaaa acaatgctgg cggcagattc gaagacctca tcagtcactt
      241 ccattccgcc atgtaggcca aactcttccg ccattttctt accaagcgtc gtctggtcgt
      301 catgaaacgc gggcaggcag tggaggaatt tgacctccgg gttaccggtc aactgcatca
      361 tcttgctgtt cacctgataa tcacgcagta atgcaatgcg ttcagcccat ttttcttttg
      421 cttcacccat cgacacccat acatcggtat agataaagtc agcaccttca actcctttag
      481 ctacatcttc cgtcagcgta atattcccgc cattttgttg tgccagggcg cggcattccg
      541 taaccagcgc agcttctggc cagcatgcct gtggcgcgac cagacgcaaa tccagaccgg
      601 taagcgccgc agcttcgagc atcgaattgc ccatgttgtt acgtgcgtca cctgcataga
      661 ccagcgtcat ttcgttgaat gctttaccgg gtaaatgctc ctgcatggtg aggagatccg
      721 ccagcaactg cgtggggtga aactcattgg tcaggccgtt ccataccggc acgccagcgt
      781 attccgccag tgtttcgaca atctcctgac catagccgcg atactgaata ccgtcataca
      841 tgcgaccaag cacgcgggca gtgtctttaa tcgactcttt atgaccaatc tgactaccgc
      901 ttgggccgag ataagtaacg cgagcaccct ggtcatatgc ggcaacttcg aaagagcatc
      961 gggtacgggt cgagtctttt tcgaagatga gcgcgatgtt tttaccagtg agtttggctt
     1021 cttctttacc gcttttctta tcagctttca gcttcgcggc taactgcagc aggctgttaa
     1081 gttcagctgg cgtgaaatcg agtaatttca ggaaatgctt atgataaaac ccggacatag
     1141 atccctcctg tggctaacgc ctcaatgaat taaaattcaa tttatatgga tgattattca
     1201 tttgcaagtc taaagcataa atctttatca caaaggtgga ggcaatgtca gtggtgtgtg
     1261 acaataagag tatcggcagg acattaagag gaatgagcca tggcaaaccc ggaacaactg
     1321 gaagaacagc gtgaagaaac acgtttgatt attgaagaat tactggaaga tggcagcgat
     1381 ccggacgcgc tgtacaccat cgaacaccat ctttccgcag acgatctgga aactctggaa
     1441 aaagcggcag ttgaagcgtt taaactcggt tacgaagtga ccgatccaga agagctggaa
     1501 gttgaagatg gtgatatcgt gatttgctgc gacatcctca gcgagtgcgc gttgaatgcc
     1561 gatctgatcg atgcccaggt tgaacaactg atgacgctgg cggagaaatt tgacgttgag
     1621 tacgacggtt ggggcactta ctttgaagat cctaacggcg aagatggcga cgatgaagat
     1681 tttgtcgatg aagacgatga cggggttcgc cactaattaa ttgattaaaa tgcccgatag
     1741 cacatactag cgggcatttt ttcagagttc gcgcaacatc cgcacttcac aatcaacatg
     1801 gcccgtgcag ccaagtgcat agtcgatatg ttgaaaacct aaatgttcat ataaaccaat
     1861 cgcttctttc aaaaaagcgg tcgtttccag atagcagcgt ttgaaaccca tttctcgcgc
     1921 ctgctccatc gccattaagg ccagtttttt tgccagccct ttaccgcgaa tggcgggaag
     1981 aaagtacatt ttctgcagtt cgcaaatgtc cgcttcacta cctgttaatg gcgctatccc
     2041 accgccgccg accacttcac cctcgtactc aaccacccaa tatgcatggc caggctgact
     2101 atacacttga tacagctcgt caagattcgg atcggcgacg gtgtagcctt tatcagcggt
     2161 aagaccgtat tcggcggata cctgacgaat gacgcgggcg atagcaggat tatcctgcag
     2221 cgtcaggcga cgtattacag gtgattgcgg cgcaatgtta ttcatagttt aacccaataa
     2281 aaataaagta attatacatt tgtttaatac cactctcttc ttacgtgcgc aagcgtctta
     2341 taaagcaata aagtttttgc cagcctatta ctgataagag tattgtaacg tggca
//
LOCUS       DAZKJR010000298         2382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKJR010000298 DAZKJR010000000
VERSION     DAZKJR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.300"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1371)
                     /locus_tag="ACHOWS_005207"
     CDS             complement(<1..1371)
                     /locus_tag="ACHOWS_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0050624.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            1548..2339
                     /gene="kdgR"
                     /locus_tag="ACHOWS_005208"
     CDS             1548..2339
                     /gene="kdgR"
                     /locus_tag="ACHOWS_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFQ0050625.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
BASE COUNT          695 a          556 c          627 g          504 t
ORIGIN      
        1 tgccctggat cgtggctgag tgatacgtaa accactgaca caggctgcaa tcaccgccag
       61 aatagccgca gctatcagcg agacgtgcgt accattatca ccaaactgat ttagcatcag
      121 cgccaccaga gccgcgccgc tactctgacc cagaagacga gccgtcccta acatgccact
      181 ggctccaccg ctacgttcgc gaggagcgga ggtaataatg gtgtggttat tgggtgactg
      241 gaacaagcca aatccagcac cacataagat catcggccag ataatattga tatccgcagg
      301 tgacgctggc agcagaacta gggaaaaaag ccccgcagcc atgatgaata accctaaagc
      361 acccagcaat cctgcatgta cgcgttcaat caaatagcct gccagtggag ccatcaccat
      421 tgttgctaac ggccacggtg tcagaagtaa acctgtttcg acttcactac gcccgagcac
      481 ggtttgcagg taaaagggaa gggaaaccat tgccagcatt tgtgcgcaga aagagcaaac
      541 agatgtgcaa atagaaagtg aaaacagcgg gatacgcagt aaatccaccg gtagcagcgg
      601 tacgggaaga gaaagctggc ggcgaataaa gaaaatacca acgacaacca ttaccaccag
      661 ttccgcacca atcaatgtca gcgattgccc ctgagcgaaa ccactcaatg cagtaataag
      721 caggccgaag gttaacgcgt tcatcacggc gctgggcagg tcgaaacggg gtttactggc
      781 gcgagaacca ttgggtggca gaaaacgcat cgccagaagc agggcgataa tacccaacgg
      841 tacgttgatt aaaaataacc atttccagga tgcgatggag aggattgctg cagcaattgt
      901 cggcccggca gcagaagaaa cggcaacaat aaacgagttt atgcccatcc ctctacccag
      961 aaaacgttgt ggatagatca ggcggataag tgcggtatta acgctcatca acgctgcacc
     1021 gccgaaacct tgtatgacac gcgcaagggt gagcatttgc agcgaatcag aaagggcgca
     1081 gaatagtgaa gacaacagaa aaacgaccag accgcattta taaatacgtc gatagccaaa
     1141 catatcgccc agaaacgaaa acgagagcag ggagatgaca atggcgattt gataggcgtt
     1201 cactacccag atggaactgg ctggcgtggc atgaaggtcc gtggcgattg ttggcaaggc
     1261 gacgttggcg attgcgccgt caaggacagc catcgaaata ccaatcacaa tggttaatat
     1321 cgcaccgtat cgctggggca atggcaggcc gtcggcctga acttttggca taggaatttt
     1381 atatctttgg tgaataatca acaggggtat gattctagca ctgatattca cggtggatgt
     1441 cgcagaattg taactaaccg gcaaaagatg gattgcgacc accctggtgc gaaattataa
     1501 taaaaaacag ttctgatttt tataaaacac tcgcaatgag gtgataaatg gctaacgcag
     1561 atctggataa acagcctgat tctgtatctt ccgtgctaaa agtttttggc attttgcagg
     1621 cgctgggtga agagcgcgaa atagggataa ccgagttgtc gcagcgcgtc atgatgtcaa
     1681 aaagcaccgt ttatcgcttt ttacagacca tgaaaacctt aggttatgtg gcgcaggaag
     1741 gggagtcgga gaaatattcg ctgaccctga aattgtttga actgggcgct cgcgcgttac
     1801 aaaacgtcga tttaattcgt agcgcagata tccagatgcg tgagatctcc cgcctgacca
     1861 aagaaactat ccacctcggc gcactggacg aagacagtat tgtttacatt cacaaaattg
     1921 actctatgta caatttgcgc atgtattcac ggattgggcg tcgtaatccg ctgtacagca
     1981 ccgcgattgg taaggtactg ctggcatggc gcgatcgcga tgaagtgaag caaattcttg
     2041 agggcgtgga gtataaacgc agtaccgagc ggaccatcac cagtacagaa gcgttattac
     2101 ccgttctgga ccaggtgcgc gagcaggggt atggcgaaga taacgaagag caggaagaag
     2161 ggctgcgatg cattgcggtt ccggtatttg atcgctttgg cgtggtcatt gccggtttga
     2221 gcatctcctt cccgacgtta cgtttctctg aagagcgttt acaggaatat gtcgcaatgt
     2281 tgcataccgc ggcgcgcaaa atttctgccc aaatgggtta tcacgactat ccgttctgat
     2341 gagagtaaga acctgtctga atatcaaaca gacaggttct tt
//
LOCUS       DAZKJR010000299         2342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKJR010000299 DAZKJR010000000
VERSION     DAZKJR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.301"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..261
                     /locus_tag="ACHOWS_005209"
     CDS             <1..261
                     /locus_tag="ACHOWS_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050626.1"
                     /translation="PDGFLVLRSVLSDDGIPYVLVLLGVCTGSNSVERYLPEVKTLTR
                     LAGGRWAEFHTARRGFIRLGKRLGFERMSDDEDGFMVFRIAV"
     gene            355..1011
                     /locus_tag="ACHOWS_005210"
     CDS             355..1011
                     /locus_tag="ACHOWS_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050627.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            1014..1460
                     /locus_tag="ACHOWS_005211"
     CDS             1014..1460
                     /locus_tag="ACHOWS_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050628.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAV"
     gene            1470..1721
                     /locus_tag="ACHOWS_005212"
     CDS             1470..1721
                     /locus_tag="ACHOWS_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0050629.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            1732..>2342
                     /locus_tag="ACHOWS_005213"
     CDS             1732..>2342
                     /locus_tag="ACHOWS_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050630.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIG"
BASE COUNT          595 a          512 c          720 g          515 t
ORIGIN      
        1 cctgatggct ttctggtgct gcgttctgtg ctcagtgacg atggtatccc ttatgttctg
       61 gtgttgctgg gcgtgtgtac ggggagtaac agcgttgagc gttatctgcc ggaggtgaag
      121 acattaaccc gtctggctgg cggacgctgg gctgaatttc atacggcaag gcggggattt
      181 atccggctgg gaaaacgtct gggctttgag cgaatgtcgg atgatgagga tggcttcatg
      241 gtgttcagga tagcggtctg actgctacag ttttcatcat tgtgtttaaa ccaacattgt
      301 aattcacatt ctgaccctgc tccggcaggg ttttttgtta tccagggggc cattatgggt
      361 ggaagtaaag gcggtggtga taccaaagta aaaccaacag cagcgcaaat agcacaggaa
      421 gaagtggcct ggaaagggtg gcaggattat caaaaaatcc tccgcccggc tgaagataac
      481 ttcatggaaa aggtcgatga tctgaacagt gagcagcagt acgacaatat tgctggcacc
      541 acaaatctgg ggtatcagaa acagtttggc gaagcacgga aggagctggc gggtaatctt
      601 gctcagtccg gtgttgaccc gtccagtggt cgttttaacg cggtaatgaa tgcgaaccag
      661 agtgatcagg taaccgggca gattgacaca accacacggg ggcaggtatc gcaggcagat
      721 aagtatgttg ccgggctaca ggatgttgca gctctcggtt ctggtcagaa ggcggatgcg
      781 ttacagagtt ttaactcgct ggcagacagc agtctggcaa aagctaaatc ggacgcacag
      841 gctgcgttta cgaaacagca ggggcgagcc tctcttgttg gcgctggttt gggtgcggca
      901 ggtgcatatg cgatgcataa ggctggcggt agcggaggaa gtggcggtgc taaaacacct
      961 ggcaccggcg ctaatgccat tcagcatcag gctcagaact ggagactgtg attatggagt
     1021 acggtaaata cgaaacactt gcgagatacg gttataccgg agctgcccgt ccacagggtg
     1081 actggcagac gtcagcagcg ctgacacgcc aacaatacga cgactggcga accagatatt
     1141 tgccccgcgt ggcaaggctg gctgaccttg gcgagaacaa cagcctgatg aatgcacagc
     1201 ttgcccgggt gggcggcctt gccacttcca gtctccgtac agcgcagatg gcgcaggata
     1261 accagatggc gagatacggg gtaagccgcc cggataatcc cgacagtaat acgctggggt
     1321 tacgtaatgc cctggcaatt gctggcgcga aaaatggtat ccgtgaagcc gaacaggatc
     1381 gccagatgaa tattctgacg ggggcttctg caccggcaag acagaaactg agtgttggcg
     1441 gacaactggt ggcagtgtaa gggggcaata tgggatacgg tttactggat attgcaaatc
     1501 agtcgcggcg tgaggcatta cagggaataa gtgacgcaga cagacgacgc acagagattg
     1561 aggcatcaaa taaacaactg gctgctcaac agaaagctca gaaaaaacag aatatcggta
     1621 cgggcattgg tacgggggcg gctattggcg catccgttgg tggtcctgtt ggcgctgttg
     1681 ctggtgcagt aattggcggc attgctggtt ctttgtttta aggagtggtg aatgagcgga
     1741 tttgcacagg ggttacttgc cggattcagc actgttgacc aggcaatgac ccgtcgtaag
     1801 gagcttggtt tgcgagaagc acagcttgct cagcaacaga aaaataacga gcgcgatttt
     1861 gaatttgcgc agtctcagtt tgaacataat aaagacgttg atcagcggaa ctttgattac
     1921 agagccaaag ttgatgaccg caattacaca ctgcaggaaa gggagtttaa cgccaaccag
     1981 aattaccgga atgcgtcgct ggggatggaa cagcaacgac tccagttgca gaaatacaac
     2041 cagcgacggc ttgagtataa cgatatgctg gcgcgcgatc agcctgtgat ggcggcgctg
     2101 ggaaaagcga ttgatgcggg tgaccatgat gcggcttctc atctgttcgg taaattatcg
     2161 gacgccaatc cgcttaagat gatgtcaacg gtaggctatg ctgcgaaagc gggtcaggcc
     2221 gtgaacaacc tgcagaaaat ctttgatgat aagccggaca gggcgattgc ttcgctgaat
     2281 accccggaaa atctcgatgt gctttccggc gtgtttgccc cggaactaca acagcgtatt
     2341 gg
//
LOCUS       DAZKJR010000300         2334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKJR010000300 DAZKJR010000000
VERSION     DAZKJR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.302"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2334
                     /locus_tag="ACHOWS_005214"
     CDS             <1..>2334
                     /locus_tag="ACHOWS_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFQ0050631.1"
                     /translation="AAEIMQRVRNSGKQAQYDRLAGIIDDMLARRREIIREAGLEESG
                     VVDAWQKAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQAMGRASRAQSPSTQAI
                     QDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRTIAERKDQETGETI
                     REVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTLGKV
                     NRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAVVKDSR
                     SAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLA
                     KGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAGLSRQQAASLAKNMTVN
                     FNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGDGPLLERLRWKNLNVPQKIAL
                     AAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIMKSMFGGKAGEYWSIPL
                     PYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGILGAFSPIGSETSETLSGALLKNA
                     APTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRSTPEAYKAFASWLNAFS
                     GGSQYRSGAVDITPESLKYWVDYISGGTGRFISKTTDAAVKSLNGIDIPEQQVPFLGK
                     ISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLMQDTRK
                     RLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAV"
BASE COUNT          576 a          555 c          729 g          474 t
ORIGIN      
        1 gcggcggaaa tcatgcagcg tgtgcgtaac agcggcaaac aggcacagta tgaccgtctg
       61 gcagggatta ttgacgatat gctggcccgt cgccgtgaga ttatccgtga ggcaggactg
      121 gaagagagcg gcgtggtgga tgcctggcag aaagcctacc gttactacgt tcccctgaaa
      181 gggcaggatg ttgacggtgt ggtgtcactg ccacgtacag gcaagggatt caccatcggt
      241 ggacgtgaaa gcaggcaggc catggggcgt gcatcccgtg cacagtctcc gtccactcag
      301 gcgatacagg acttgagcga atcgctgatc cgccatcgca aaaacgaagt gggtaacgcc
      361 ttcctgaaac tggtgcagga taatcccgac aaggattact ggcaggtatt caccgatgac
      421 agaccggata ccatgcggac gattgcagag cgcaaggacc aggaaactgg tgaaaccatt
      481 cgcgaagttg tcgaacgccc tgtaccgatg gcaatgatgg cagaccggta tttcaccacc
      541 aaaaagaacg gcaaaacgta ctacatcaaa ctccatgacc cgcgcctgat gcgtgcgatg
      601 aagaatatgg gaccggaaac cagtaacgcc gtaatccgca cgctggggaa agttaaccgc
      661 ttcctggcaa cggtgaacac gtcgtataac ccggaattcc tggtcagtaa cttcatccgt
      721 gacgtgcaga cggcggtgat gaacctgaag gcggagcagg gaaggagcga cggcaaactg
      781 aaagggctgg ataacttatc cgccctggct gtagtcaaag acagccgttc tgccatgtca
      841 gccgtatacg ccagtctgcg tggtaaaacc ctcacgggca aaggtgcaca gtggcagaag
      901 gtgtggaaag agtttgttga ggacggaggc aaaaccggct ggtttaacat gggtgacctt
      961 gaaggccagc agaaggaaat ggatcgtctt gtgtcgctgg cgaagggggg atggaaaggc
     1021 cagagtatcg gtgcatggaa ttcgttcctg aacctggtcg aggatgccaa cggcgcggtt
     1081 gaaaacgcac tgcgtctgtc agcttataaa cacgcccgtg atgccggttt gtcacgccag
     1141 caggcggcgt ctcttgccaa aaacatgacg gtgaacttta accgtcgtgg tgagcagggg
     1201 gcgctgatga actcgctgta catgttcgct aacgccagca ttcaggggac ggcaaacctt
     1261 gtgagaaccc ttgggcacct taatggtgac ggtccgttac tggagcgcct tcgctggaag
     1321 aatctgaatg tacctcagaa aatcgcgctt gctgctgtgg gagcgggtta tctgcttggc
     1381 tcgcttaacc gcagcgtggc gggtgaggat gatgacgggg ttaactggta tgacaaggtg
     1441 ccgtctcatg tgaaagagcg taacctcgtc attatgaaat cgatgttcgg gggcaaggcc
     1501 ggagagtact ggagtattcc tctgccttac gggtacaacg ttttcttcct gctcgggcat
     1561 accgctgaag gtgtggcggc gggtgacctg acggcgtccc gtgctgccgg taatgttgtc
     1621 ggtggtatcc tgggagcatt cagcccgatt ggcagtgaga cgtcggaaac actgtccggg
     1681 gcattgctga aaaatgcagc gccgaccatt ctgcgtccgt ttgcgaacct tgccatgaat
     1741 gaaaacttca tgggggcgca gatttaccag gagaacatgc cgtttggtac accaaaacct
     1801 gacagccagc tgggaagacg ttcaacgcca gaagcgtaca aggcgtttgc atcctggctg
     1861 aatgcgttct ctggtggcag ccagtaccgc tcaggcgcgg tggatatcac accggaatcg
     1921 ctgaaatact gggtggacta tatctctggt ggtacagggc gcttcatttc caaaaccacg
     1981 gatgcggcgg tgaaatcgct gaatggtatt gatataccgg agcagcaggt gcccttcctg
     2041 gggaaaattt cgggtgaggt gatgccgtat gctgaccagc agaagatgta cgaccggatg
     2101 acggaggttg cgcagtatca cgcagagctg aagagtctga ccggtgcaga aagaacggcg
     2161 ttcattgacg agaacaacgg aaaattgtcg atgaacgggc ttatgcagga tacccggaag
     2221 agactgaagg atttgcgtaa acagcgtgat gccatttacg ccgacagtac tctcagtctg
     2281 gcgcaacagt cggcgatggt gaaatcggta gagcgggata tgaaaattgc cgtg
//
LOCUS       DAZKJR010000301         2327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKJR010000301 DAZKJR010000000
VERSION     DAZKJR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.303"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..2264
                     /locus_tag="ACHOWS_005215"
     CDS             123..2264
                     /locus_tag="ACHOWS_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFQ0050632.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
BASE COUNT          640 a          553 c          554 g          580 t
ORIGIN      
        1 cgtgattgaa tgattctcaa ttacatatcc gatggcgaaa taacaaacgg atagtgctta
       61 gtgagcaata agaaacaatt ttcactcgcg acaatccgcg agtttttggg ataacagact
      121 aaatggctat gttcacacct tcattctcag gactcaaagg tcgggcgctc ttttcactgc
      181 tttttgcggc accgatgatt catgcaacag actctgtaac gaccaaagat ggcgaaacaa
      241 tcactgttac agcagatgca aataccgcaa ctgaggcgac cgatggttat caacctctga
      301 gcacttccac ggcgacatta accgatatgc cgatgctgga tatcccgcag gtggtcaata
      361 cggttagcga tcaggttctg gaaaaccaga atgcgacaac gctggatgag gcgctttata
      421 acgtcagtaa cgtggtacag accaatacat taggcgggac tcaggatgcc tttgtacgcc
      481 gtgggtttgg cgcaaaccgg gatggctcca tcatgaccaa cggtctgcga accgtacttc
      541 ctcgcagttt caacgccgcc acagaacgtg tggaagtgct aaaaggcccg gcctccacgc
      601 tgtatggcat tctcgatcct ggcggactga ttaacgtcgt gaccaagcgc ccggaaaaaa
      661 cattccatgg ttcggtttca gccacctcct ccagttttgg tggcggcact gggcaacttg
      721 atatcacagg tcccattgaa ggcactcagc tggcgtatcg ccttaccggg gaagtgcagg
      781 atgaagatta ctggcgaaac ttcggtaaag agcgcagtac atttattgcc ccgtcactca
      841 cctggtttgg tgataatgca acagtaacca tgctctattc ccatcgggac tataaaactc
      901 cgttcgatcg tggaacgatt ttcgacctta cgacgaaaca gcccgtaaac gttgatcgaa
      961 aaatacgttt tgacgaaccg tttaatatta cagatggtca gtccgatctg gcgcaactca
     1021 acgcagaata tcatctcaat agccagtgga cagcgcgctt tgattacagc tacagccagg
     1081 ataaatacag cgataatcag gcgcgtgtta ccgcgtatga tgcaacgaca ggaacactga
     1141 cacggcgtgt tgatgcaact cagggatcta cccagcgtat gcatgctact cgtgcggatc
     1201 tgcaagggaa tgttgatatt gccggattct ataatgagat tctgggtggg gtgtcatatg
     1261 aatattatga tcttctgcgt acagatatga ttcgctgtaa aaaagctaaa gatttcaata
     1321 tctacaaccc tgtttatggt aataccagca aatgtacaac ggtttcggcg tcggacagtg
     1381 atcagacgat caaacaggag agctactcag cgtatgcaca ggacgcactc tatctgaccg
     1441 ataactggat tgccgtcgcc gggatccgct atcagtatta cacgcagtat gcgggtaaag
     1501 gccgtccttt taatgtcaat actgacagcc gcgatgaaca atggactccc aaactggggt
     1561 tggtctacaa actgacgcca tcggtatcct tatttgccaa ttattcgcaa acatttatgc
     1621 cgcagtcgtc aattgccagc tacattggcg atcttccacc ggaatcatct aatgcttacg
     1681 aagtcggggc aaaattcgag ctattcgatg gtatcaccgc agatattgcg ctgtttgata
     1741 tccataaacg taatgtgttg tataccgaaa gtgttggtga tgaaaccatt gccaaaacgg
     1801 caggccgtgt tcgttcaaga ggggtagaag tcgaccttgc gggagcatta actgaaaaca
     1861 ttaatatcat tgccagctac ggctataccg atgctaaggt tctggaagat cctgattatg
     1921 caggtaaacc attgccgaat gttcctcgtc ataccggttc gctattcctg acctatgaca
     1981 ttcataacat gcctggcaat aacacactga cgtttggcgg tggtggacat ggcgtaagcc
     2041 gtcgttcggc aaccaatggg gctgactatt atctgccagg ctattttgtt gccgatgcct
     2101 tcgccgcata caaaatgaaa ttgcagtatc cggtcacact gcaattaaac gtcaaaaacc
     2161 tgtttgataa aacgtattac acctcttcca tcgccacaaa taatctgggg aatcagattg
     2221 gcgatccgcg tgaagtgcaa ttcacggtga aaatggaatt ttgatcaaaa aagcccggta
     2281 tgtttaccgg gctgagactg atgacaaact ccaaattgtc tgatgcg
//
LOCUS       DAZKJR010000302         2293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKJR010000302 DAZKJR010000000
VERSION     DAZKJR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.304"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1612)
                     /locus_tag="ACHOWS_005216"
     CDS             complement(<1..1612)
                     /locus_tag="ACHOWS_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050633.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAEATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVVGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLVKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELEEQ
                     AKRQAVMNELKAKPRSELLEEYRRLSQKE"
     gene            complement(1682..>2293)
                     /locus_tag="ACHOWS_005217"
     CDS             complement(1682..>2293)
                     /locus_tag="ACHOWS_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050634.1"
                     /translation="IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTV
                     LAIPVDKAIAQVRDRSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNA
                     QAAVDEYYAATGSQPHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQN
                     QKLLENGYANFLRIQKARGEQARDESASSASQFIRGLKQNYAQ"
BASE COUNT          473 a          701 c          531 g          588 t
ORIGIN      
        1 cctctttctg gctgagtctg cggtattcct caagcagttc agaacgcggc ttcgccttca
       61 gctcattcat cacagcctgt cgcttcgcct gttcctccag ttcattcaac aactggctgg
      121 cagcttcccg gcgatgagct gcggatgcgt caccctctgt tgccatatcc gcatcagcat
      181 actgctccag aagctgctcg cgattcatgc cctgcatgga ttcacgctgc tgcgccaccg
      241 gatcaacaga ttctggttgc ggggctgcgt cgtcctgctg cacggtttca gcatcacgca
      301 tggcggtttc ttctgcggcc tgacgtctgc cacgatatcc ggcaaccgca ccggatggag
      361 cccccatcgc agcaccaaag gctgcacctt cgatcgttgc gtcagccacg ccctcccacg
      421 gtgacacatc cattccggcg gtctcacgca atgccgtgtt ttcctgatag cgtgaatagc
      481 cgccctgcgc cgcattaatc gccccctgtt ccgtggcatt tctgacaatg ccgcttttaa
      541 cggtcttcgc tgtgcctcgt gtcaccagat taaacagttg tgcgtcaccc agttttgccg
      601 ccatggcatt cacagccagc aattcaggat cggttgccag ctgcgcgcgc acctcatcgg
      661 caacacgctc ttttaccaga tccattttct ggcgatcagt aagctgtgcg tgctgcgggt
      721 cggcgtcaat ggacaaaaac gtctgctgaa atttcggtga ctgcgccagc tcagagtaat
      781 ccgcattaag aacagcatct gctgccgcca ttgcactctg cccctgtgca ctggctgtgg
      841 aatgggtgat caggcccgcc tggaataaat ccggcatttt tttatcgaca gcttctgctg
      901 ccagtgccgt ggctctttcc ggctgcatcc ctgccgcgat gtattttttc tccagcccgg
      961 cggtcagcat ttttcgcagt gtgacatcac ccaccttttt agccacaccg ccaacaacca
     1021 tatcaggtac aagcgcacca atcaggttta cacccttcgc cacccagacc gcagaatcat
     1081 catagccttc ggtcatcggc gtattcagcg cacgaactgc acccggcgac atcttaccgg
     1141 tcagccattc atcagtggct tcagcaccat cactgacagc cttaccggtt gccttcagac
     1201 ctttaccgac agtatcagta accgcgtttt tgccatcagg cagggtatcg atgacctcat
     1261 cagcccccct gctgccaccg gcaaaaatat cctgcacagt tgcgacaccc ggcagcccca
     1321 tacggctgaa ctcatttaaa atacgcgccc ctgtttttac cgggctctga atcatcgcat
     1381 caccgagtcc acgggccatt tcccctgttc cccggacgga ctgggcgaaa cctttaccca
     1441 ttgttggcaa tacatcgccc aggctgaacg acgtactgtt atccttccag cgattcggat
     1501 cagagaaaaa tgcttcatag ctgtcagttt cgccgggttg ctgaatgttc aggctgttac
     1561 gattctggtt accgagttgc gcctcaggac gctgttcctc tgaataggcc atacagactc
     1621 cataaaaaaa cccggcacaa tggccgggca tcaggaaatg gtattaatgg aatatcgtga
     1681 attactgggc gtaattctgt ttcagtccac ggataaactg agatgcagat gaagcacttt
     1741 catctctggc ctgttccccc ctggcctttt gaatacggag aaaattcgca tacccgttct
     1801 ccagcaattt ctgattctga ggttccagca tttcaggctg acgggccgct acatttctgg
     1861 caaatgacag cttatcagga tcatctcccg cccagttgat aacctgttgc tgaagttttt
     1921 gttgctgaat tttctgttga tgcggttgtg aaccagtcgc agcataatat tcatccaccg
     1981 cagcctgagc attaccgcca ttcttgatcg cgtccgccgc tacgttgctg gcccccttct
     2041 gaagctcttt cagagaaagt ccctgctgct tcggcataaa ataaccataa tttttcgata
     2101 tctctgcaaa tttgctgcga tccctgacct gagcgatagc cttatcaacg ggtatcgcca
     2161 gcacggtttg atcatcaggg tgcgcactgc cgtattctgt tacaggttta tgcgcggtgg
     2221 agccatcgct gtatgtgaga tcaaggccaa taagtacgta cccttcctgc tgcgccggta
     2281 cgatactgcc aat
//
LOCUS       DAZKJR010000303         2285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKJR010000303 DAZKJR010000000
VERSION     DAZKJR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.305"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..109
                     /locus_tag="ACHOWS_005218"
                     /pseudo
     CDS             17..109
                     /locus_tag="ACHOWS_005218"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            182..>2285
                     /locus_tag="ACHOWS_005219"
     CDS             182..>2285
                     /locus_tag="ACHOWS_005219"
                     /inference="COORDINATES: protein motif:HMM:NF017476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFQ0050635.1"
                     /translation="MATAVGFNALANASNALAVGSQASADGEDSLAIGSQSTTGKKST
                     VALGQGATASAAEGNVAIGADSVDKAATPVSGATIKLKNGGTIEYKGFAGDKAASVVS
                     VGDAGKERQIVNVGAGAISDTSTDAINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGS
                     ATINGGKDNKNGNTLTVKGDGDIAVTVNDKGMTIDGTTLKNEIKNDAIKIKGGDGKTL
                     TKSLGDNVEITGKGPIKTQVAGDKLEISAETASLPVNGGNGKITVNNGDGDKLVTAKN
                     IAEAINQSGWKIKADKDTGGVIEGNGQGTETLIKPGDMVTLKAGKNLSVKQSGGEFTF
                     SVIDTPSFSSVQLNGNGKNGNVTLSSEGNKLTLSGGNGAGQNGVVISGVASGLEGKEL
                     STLTNTDKEMKNAASVADLKTAIDGVSTSMQGAGFALKDSKGQEVKQSLGQSIKVSGD
                     SNITTTAITNGDKGLKISLNNNLSVGSNNTPGSIKVNGADGKEAVSLNGQDGTIKLTS
                     APAANGKSASATIAVNAGKGGLTDAAGTTKTRLTYAPDGTLPAEELATMNDGLSFEGD
                     DGKVIHKKLNEKLTIKGGAGDNEDVTDSNIRVDANEKGDGLLVRMTRNLRDLASAVFG
                     TGGQITTVNDKGITITTPAAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALADAG
                     GDVLNNAANIGDLKNALRD"
BASE COUNT          681 a          431 c          660 g          513 t
ORIGIN      
        1 attcggtgat aaagaaattc cgaataatta ctcgtcatct gaagcatcag gagacggaag
       61 tattgttgtg ggctcaagga gcacagctgc aggagacctg tctgtgtccc tgggctcaat
      121 aagctctgcc aacgggacgg ctgctaatgc actgggagcc acggcggaag ctaacgggga
      181 tatggcgact gcggttggtt ttaatgcgct ggctaatgcc agtaatgctc tggctgttgg
      241 tagtcaggcc tctgccgacg gagaggattc cctggctata gggagtcaga gtacgacggg
      301 caaaaagtcc acggttgctc tggggcaggg agctacagcc tctgccgcgg aggggaacgt
      361 ggccatcggt gctgattcag tggacaaggc agcaactccg gtttccggcg ctaccattaa
      421 gttaaagaat ggcgggacta ttgaatacaa gggctttgcc ggagataagg ctgcatcagt
      481 agtatctgtt ggtgatgcgg gtaaagagcg tcagattgtt aacgtggggg cgggtgcgat
      541 ttctgacaca agtacagatg ccattaacgg tagtcagtta tatgcgatag cgaagacact
      601 gaaagatgac cttgatgcca ttaatccgac gtggacgcta aaggatgata agggaggttc
      661 tgcgactatt aatggtggga aggataataa gaatggtaac actttaactg ttaaaggtga
      721 tggagatatt gcagttactg ttaatgataa aggaatgacg attgacggaa caacgctgaa
      781 aaatgaaata aaaaatgatg ccatcaaaat taaaggtgga gatggtaaga cgctgacgaa
      841 gagtcttggt gacaatgtcg aaatcactgg caaaggccca ataaaaaccc aggtggctgg
      901 agataaactg gagatttcag cagagacagc ttcgttgcct gtaaatggtg gtaacgggaa
      961 gattacagta aacaatggtg atggcgataa acttgttact gcgaaaaaca ttgctgaagc
     1021 cataaaccag tctggctgga aaattaaagc ggataaagat accggtggtg ttatcgaggg
     1081 aaatggtcag ggaacagaaa cactgataaa acccggtgat atggttacgc tgaaagccgg
     1141 taaaaatctc agcgtgaaac aaagtggtgg tgaatttaca ttctctgtta ttgatactcc
     1201 gtcattcagc agtgttcagc tgaacggtaa tggaaaaaac ggaaatgtaa cgctctcatc
     1261 cgaaggtaat aagctgacat tgtcaggcgg taatggtgcc ggacaaaatg gtgttgttat
     1321 cagtggtgtg gcaagtggac tggaaggtaa ggaactttcg acgttaacga atactgataa
     1381 agagatgaaa aatgccgcca gtgttgcaga cctgaaaacg gcaattgatg gtgtgtccac
     1441 cagtatgcag ggagcaggtt tcgcactgaa agattctaag ggacaggaag tgaaacagtc
     1501 tcttgggcag tcgattaaag tatccgggga cagcaatatc accaccacag ccatcaccaa
     1561 tggggataag gggttaaaaa tatccctgaa taataacctg agcgttggga gcaataatac
     1621 tcccggcagc atcaaagtta acggggctga tggtaaggag gctgtgagtc tgaatggtca
     1681 ggatggcacg attaaactga catccgcgcc tgctgcgaac ggaaagtctg cgtcagcaac
     1741 cattgctgtt aatgcaggca aaggtggatt aacagatgct gccggtacca cgaagacacg
     1801 tctgacttat gctccagatg gaacattacc ggcagaagag ctggcgacca tgaatgacgg
     1861 tctttctttt gagggtgatg acggaaaggt tattcataaa aaactgaatg aaaaactgac
     1921 catcaaaggt ggtgctggtg ataatgagga tgtaacagac agtaacattc gtgttgatgc
     1981 aaatgaaaag ggagatggtc ttctcgtccg gatgaccaga aacctccgtg atttggccag
     2041 cgcggttttt ggtacaggtg ggcagataac aacagttaat gataaaggca tcacaatcac
     2101 aacaccggct gcgggcggtg gtcaggggaa tacagtttcc cttaccggct ccggcctgaa
     2161 taacggtgga aatcagataa ccagtgtggc cagtggtctg aacggcactg cgcttgctga
     2221 cgccggtgga gatgttctga ataatgcggc caatatcggt gacctgaaaa atgctctccg
     2281 tgaca
//
LOCUS       DAZKJR010000304         2273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKJR010000304 DAZKJR010000000
VERSION     DAZKJR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.306"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            79..969
                     /gene="alsR"
                     /locus_tag="ACHOWS_005220"
     CDS             79..969
                     /gene="alsR"
                     /locus_tag="ACHOWS_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFQ0050636.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            1028..1963
                     /gene="alsB"
                     /locus_tag="ACHOWS_005221"
     CDS             1028..1963
                     /gene="alsB"
                     /locus_tag="ACHOWS_005221"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFQ0050637.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGETRRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2091..>2273
                     /locus_tag="ACHOWS_005222"
     CDS             2091..>2273
                     /locus_tag="ACHOWS_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0050638.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGT"
BASE COUNT          630 a          500 c          581 g          562 t
ORIGIN      
        1 ttgtgaagtt gccagcatct tttctgttct tgctgtggtg atatagtggc gtcttcaatt
       61 caaggacaag agaacgtgat gagccagtca gagtttgatt cagcgcttcc gaacggtatc
      121 gggttagcgc cttacctgcg aatgaagcag gaaggaatga cagaaaacga aagccgcatc
      181 gtggagtggt tactcaaacc cggtaacctg agttgtgcac ccgcaattaa agatgtcgca
      241 gaagctctgg cggtatctga agcgatgatc gttaaggtat caaagctgct ggggtttagc
      301 ggctttcgta acttacgcag tgcgctggaa gattattttt ctcagtcaga acaagtattg
      361 ccttccgagt tggcttttga tgaagcgccg caggatgtgg tgaataaggt atttaacatc
      421 actttacgca ccattatgga aggtcagtcg atcgtcaacg ttgatgagat ccaccgtgcc
      481 gcccgctttt tctatcaggc cagacagcgg gatttgtacg gtgccggagg atcaaatgca
      541 atctgtgctg atgtacagca caagtttttg cgcattggcg tacgctgcca ggcctatccg
      601 gacgctcaca tcatgatgat gtctgcttcg ttgttacagg aaggagatgt ggtgctggtg
      661 gtgacccatt ccgggcgaac cagtgatgta aaagcggctg tagaactggc aaaaaagaac
      721 ggggcaaaga ttatttgtat aacgcatagc taccattcac cgatagcgaa gctggccgat
      781 tatattattt gctcaccggc cccggaaaca ccgttattag gtcgtaatgc ctcggcaaga
      841 atattacaac taactttgct ggacgcgttt tttgtctctg tcgcccagct caacattgaa
      901 caagctaata ttaatatgca aaaaaccggc gcaattgttg atttcttctc accaggcgcg
      961 ctgaaataag aaaaaggtaa ttccgccatc gcggagttac ctatcccctt accgtgagaa
     1021 catgattatg aataaatatc tgaaatattt cagcggcaca ctcgtgggct taatgttgtc
     1081 aaccagcgct tttgctgccg ccgaatatgc cgtcgtattg aaaacactct ccaatccatt
     1141 ttgggtagat atgaaaaaag gcattgaaga tgaagcaaaa acgctgggcg tcagcgttga
     1201 tatttttgcc tctccttcag aaggcgattt tcaatctcaa ttgcagttat ttgaagatct
     1261 cagtaataaa aattacaaag gtatcgcctt cgcaccatta tcctcagtga acctggtcat
     1321 gcctgtcgcc cgcgcatgga aaaaaggcat ttatctggtc aatctcgatg aaaaaatcga
     1381 catggataat ctgaaaaaag caggcggtaa tgtggaaggt tttgtcacca ccgataatgt
     1441 tgccgtcggg gcgaaaggcg cgtcattcat tattgacaag ctgggtgccg aagggggtga
     1501 agtcgcaatc attgagggta aagccggtaa cgcctccggt gaaacgcgtc gtaatggtgc
     1561 caccgaagcc ttcaaaaaag caagccagat caagcttgtc gccagccagc ctgccgactg
     1621 ggaccgtatt aaagcactgg atgtcgccac taacgtgttg caacgtaatc cgaatattaa
     1681 agcgatctat tgcgcgaatg acacgatggc aatgggtgtt gctcaggctg tcgcaaacgc
     1741 cggaaaaacg ggaaaagtgc tggtcgtcgg tacagatggc attccggaag cccgcaaaat
     1801 ggtggaagcc ggacaaatga ccgcgacggt tgcccagaac ccggcggata tcggcgcaac
     1861 gggtctgaag ctgatggttg acgctgaaaa atccggcaag gttatcccgc tggataaagc
     1921 accggaattt aaactggtcg attcaatcct ggtcactcaa taaaactaaa tgatgccgga
     1981 tggtgatgct aatgaagtgt cttatccggc caacaaaata acctgaacgt agacctgata
     2041 agtgtagccc atcaggcaat aatacgatat ttcgtgcaga gaggttattt atggccacgc
     2101 catatatatc gatggcgggg atcggcaagt cctttggtcc ggttcacgca ttaaagtcgg
     2161 ttaatttaac ggtttatcct ggtgaaatac atgcattact aggagaaaat ggcgcgggta
     2221 aatccacgct aatgaaagtt ttatccggaa tacatgagcc gaccaaaggc acc
//
LOCUS       DAZKJR010000305         2237 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKJR010000305 DAZKJR010000000
VERSION     DAZKJR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.307"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..1123)
                     /gene="aroH"
                     /locus_tag="ACHOWS_005223"
     CDS             complement(77..1123)
                     /gene="aroH"
                     /locus_tag="ACHOWS_005223"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFQ0050639.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1280..2113)
                     /gene="ppsR"
                     /locus_tag="ACHOWS_005224"
     CDS             complement(1280..2113)
                     /gene="ppsR"
                     /locus_tag="ACHOWS_005224"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFQ0050640.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT          560 a          552 c          575 g          550 t
ORIGIN      
        1 ccagagcaag cagtaatttg ttgagaagac aacaagttgc gcagaaatgc ccatcccgtc
       61 aggaatgggc acgcattcag aagcgagtat ctaccgcaga ggcgagtttt tcgaccaggc
      121 gttcggtatc ttcccagccc agacacgggt cggtaatgga ttgaccgtaa gtgagcggct
      181 gaccgccgac gattttttgc gttccttcgc gcaggaaact ttccgccata attccagcaa
      241 tcgccgtaga tccattgcgg atttgctgac aaatatcctc acaaacttct aactggcgtc
      301 ggtgctgctt ctggcagtta ccgtggctga aatccaccac cagatgttca ggtaaatcaa
      361 actcgtgcag cgtatcgcag gctgcggcga tatcatcggc atgataattc ggttttttgc
      421 cgccacgcat aataatgtgg ccatacgggt tgccgctggt ctgatagatg gtcatctgac
      481 catttttgtc tggcgagagg aacatatggc tggcgcgggc tgcgcggata gcatccacgg
      541 caatccgcgt gttgccatcg gtaccatttt taaaacctac cggacaggag agtgccgaag
      601 ccatttcacg gtggatctga ctttcggtag tacgtgcgcc aatcgcgccc caactgatta
      661 aatcagcaat aaactgaccg gtcaccatat cgaggaactc agtcgcggta gggacgccca
      721 gctcatttac ctgtaaaagt aatttgcgcg ccagctccag accgtgattt acccgatagc
      781 tgccgtttaa atctggatcg gagattagcc ctttccagcc gacaacagtt cgtggttttt
      841 caaaataggt gcgcattacg atttccagcc gtgactggta ctggttgcgc agcgactgca
      901 gacgggtggc gtactccatt gcagcggtga gatcgtggat cgagcagggg ccaataatga
      961 ccaacagtcg cttatcttca ccattcagta ttttttcaat tctgcggcgg gagtcggtga
     1021 catgggtggc gacgccaggc gttacgggat accgtagcgc gagttcggcg ggcgttacca
     1081 ggctctcaat acgcgcagta cggagttcgt cagttctgtt cattacaagt ctcagaaatt
     1141 tgttgcttct acggcaggac taccgggaag tgatgcgcat cacgataaac caatcgcagc
     1201 tgatttcaag tgcgaggtgt gtcacacctc gccgggtggt tagcatgata acaaaaaaac
     1261 aagctaatgc actagttctc tagtacattc gacggctaag gcccatgata tcgaggatct
     1321 tggtggcaat ctcttctacc gaataattgg tactgttaat ccacgggatc tgatttttac
     1381 ggtacaacgc ttccacttcc gcgacttcca tcctgcactg acgaagcgag gcatagcgac
     1441 tgttctcccg acgttcctcg cgaatcgccg ccagacgttc cgggtcgata gtcaggccga
     1501 acaatttatg ctgaagcggt ttgagcgacg cgggtagcac aagattatcc atatcgtcgg
     1561 caataaaggg gtagtttgcc gcgcggatac caaactgcat tgccagatac agactggtgg
     1621 gggttttacc acagcgagaa acgccgagca ggatcacctg agcctggtcc agattgcgta
     1681 acgaaatgcc atcatcgtgg gcgagggtgt aatcaatcgc agcaatgcgc gcatcatatt
     1741 tattgagatt attagggtta aggccatggg tacgatgagc aatcggcgtt ggatccagtt
     1801 tcatctcttg ttgtagcggg gcaaccagcg cctgaacgat atcctggcaa aagccctcac
     1861 tttgcaagat gatggcgcga atctccggca acacgatgga gtagaagacc agcgggcgca
     1921 cacctgtctg gtgataaatt gcgtcaatct gatccttcac tgcccgtgca cggctctcat
     1981 tttcgacaaa cggcagcgta atgctgctga tagtgacggg aaattgtgac attactgcgt
     2041 gtcctaatac ctccgcagtt attgccgtac catcagaaat ataaaaaacg tggcgatcaa
     2101 cagcattatc cattttgttc ttcccgtgat gcagacataa ttctatgaaa gcataaatta
     2161 aaaacgcaca gaagcgtaga acgttatgtc tggtttataa aatgaacctt caattttatt
     2221 ttttatgaaa acagcat
//
LOCUS       DAZKJR010000306         2218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKJR010000306 DAZKJR010000000
VERSION     DAZKJR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.308"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..76
                     /locus_tag="ACHOWS_005225"
     CDS             <1..76
                     /locus_tag="ACHOWS_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFQ0050641.1"
                     /translation="GVELETERFEQTVREVQDLASQNG"
     gene            117..192
                     /locus_tag="ACHOWS_005226"
     tRNA            117..192
                     /locus_tag="ACHOWS_005226"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Ile,seq:cat)
     gene            200..276
                     /locus_tag="ACHOWS_005227"
     tRNA            200..276
                     /locus_tag="ACHOWS_005227"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:234..236,aa:Arg,seq:tcg)
     gene            290..366
                     /locus_tag="ACHOWS_005228"
     tRNA            290..366
                     /locus_tag="ACHOWS_005228"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:324..326,aa:Arg,seq:tct)
     gene            458..1417
                     /gene="stxA2b"
                     /locus_tag="ACHOWS_005229"
     CDS             458..1417
                     /gene="stxA2b"
                     /locus_tag="ACHOWS_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFQ0050642.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1430..1693
                     /gene="stxB2b"
                     /locus_tag="ACHOWS_005230"
     CDS             1430..1693
                     /gene="stxB2b"
                     /locus_tag="ACHOWS_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFQ0050643.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          570 a          489 c          549 g          610 t
ORIGIN      
        1 tggcgttgag ctggagactg aacgttttga gcagacggtc agggaagttc aggatttagc
       61 cagtcagaac ggatgatatt gcaggattag ttacgtaccg ttattatcct gcgcccggcc
      121 ctttagctca gtggtgagag cgagcgactc ataatcgcca ggtcgctggt tcaaatccag
      181 caagggccac catcacatac cgccattagc tcatcgggac agagcgccag ccttcgaagc
      241 tggttgcgcg gggttcgagt cctcgatggc ggtccattat ctgtacactg cgttgttagc
      301 tcagccggac agagcaattg ccttctaagc aatcggtcac tggttcgaat ccagtacaac
      361 gcgccatact tattttttct ggctcgcttt tgcgggcctt ttttatatct gcgccgggtc
      421 tggtgctgat tacttcagcc aaaaggaata cctgtatatg aagtgtatat tgttaaaatg
      481 ggtactgtgc ctgttactgg gcttttcttc ggtatcctat tcccgggaat ttacgataga
      541 cttttcgacc caacaaagtt atgtctcttc gttaaatagt atacggacag aaatatcgac
      601 cccacttgaa catatatctc aggggaccac atcggtgtct gttattaacc acaccccacc
      661 gggcagttat tttgctgtgg atatacgagg gcttgatgtc tatcaggcgc gttttgacca
      721 tcttcgtctg attattgagc aaaataattt atatgtggcc ggattcgtta atacggcaac
      781 aaatactttc tacaggtttt cagattttgc acatatatca gtgcccggtg tgacaactgt
      841 ttccatgaca acggacagca gttataccac tctgcagcgt gtcgcagcgc tggaacgttc
      901 cggaatgcaa atcagtcgtc actcactggt ttcatcatat ctggcgttaa tggagtttag
      961 tggaaatgcc atgaccagag atgcatccag agcagttctg cgttttgtca ctgtcacagc
     1021 agaagcctta cggttcaggc aaatacagag agaatttcgt ctggcactgt ctgaaactgc
     1081 tcctgtttat acgatgacac cggaagaagt ggacctcaca ctgaactggg ggagaatcag
     1141 caatgtgctt ccggagtttc ggggagaggg tggtgtcaga gtggggcgaa tatcctttaa
     1201 taatatatca gcgatactgg gcacagtggc ggttatactg aattgccatc atcagggggc
     1261 gcgttccgtt cgcgccgtga atgaagagat acaaccagaa tgtcagataa ctggcgacag
     1321 gccagttata aggataaaca atacgttatg ggaaagtaat accgcagctg cttttctgaa
     1381 tcgcagggct cactctttaa atacatccgg agaataacag gagttaaata tgaagaagat
     1441 atttgtagcg gctttatttg cttttgtttc tgttaatgca atggcagctg attgtgcaaa
     1501 aggtaaaatt gagttctcta agtataatga gaatgataca ttcacagtaa aagtggccgg
     1561 gaaagagtac tggactaacc gctggaatct gcaaccgcta ctgcaaagcg cacagttaac
     1621 aggaatgacg gtaacaatca aatcaaatac ctgtgcgtca ggttcaggat ttgctgaagt
     1681 gcagtttaat taatatcaga agcattgctg gtttcgtggt gtgcagcaat gtagttacag
     1741 tgtaatcaat gtcacaattc agtcagttga aggttgtctg cccgactgag aatttgttaa
     1801 aaaaatcctg catggtgaat ccccctgagc ggaggggcat atcagcgtca caggtgtttc
     1861 tgttttacct ctatcctttc tgtgcgggtt caggtgctga tactgaactc accgggaggc
     1921 acccggcacc atgcatgaac ggtacatagc gcatacacca gcccctctcc ggaggggctt
     1981 tcttgtgggc aaaaaaaagc ccgagtgggt tcgggcaata gcatgagata tacattttta
     2041 taatcgaatg gattttaacc aggattcata aggctgcgca actgcgcggc ctttttcgta
     2101 tttcgggctg tagtcttcct tctgccattg tcctgtaact tccggacttc agcccgcccc
     2161 ttatctgact cacaacatta tcccggccgg gaggattcat ggcatttaaa cactatga
//
LOCUS       DAZKJR010000307         2217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKJR010000307 DAZKJR010000000
VERSION     DAZKJR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.309"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2047
                     /locus_tag="ACHOWS_005231"
     CDS             <1..2047
                     /locus_tag="ACHOWS_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFQ0050644.1"
                     /translation="KSRKSVKSVEPDIADRANSWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGAGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ"
     gene            2080..>2217
                     /locus_tag="ACHOWS_005232"
     CDS             2080..>2217
                     /locus_tag="ACHOWS_005232"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFQ0050645.1"
                     /translation="MKSGGKWEEYTIERLFSIQTPKKKFNANKLTFNGNYPYVARGSN
                     NN"
BASE COUNT          691 a          422 c          534 g          570 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacagctg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcgca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggtag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat gatacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcattcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaactgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccaccgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctggcc gattttgacg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagacc aggccagtag ataccagacc gacactggag gattttaaaa agacggttgg
     1981 cgactatctt gcgtgggagg tgtcaaaact tcttcagggg cgtgatgcgt cagaaaaaaa
     2041 gcagtaaact cccgtcttgc agagcttgag cgggagttta ttaaatctgg tggtaaatgg
     2101 gaagaataca caatcgaaag attgttcagt attcagacac ctaaaaagaa atttaatgcc
     2161 aataaattaa catttaatgg caactatcct tatgttgcca gaggaagtaa caataac
//
LOCUS       DAZKJR010000308         2213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKJR010000308 DAZKJR010000000
VERSION     DAZKJR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.310"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1214)
                     /locus_tag="ACHOWS_005233"
     CDS             complement(<1..1214)
                     /locus_tag="ACHOWS_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFQ0050646.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFAFLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     LGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRL
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTG"
     gene            1712..1807
                     /gene="dqlB"
                     /locus_tag="ACHOWS_005234"
     CDS             1712..1807
                     /gene="dqlB"
                     /locus_tag="ACHOWS_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFQ0050647.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELID"
     gene            complement(1950..>2213)
                     /locus_tag="ACHOWS_005235"
     CDS             complement(1950..>2213)
                     /locus_tag="ACHOWS_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFQ0050648.1"
                     /translation="AQITAAAEDAEIHHDYARALILWQHAYHAATLTLNKNLAVAKID
                     FCIKRIRMREQMSGIIRKTGTDEILFRLSKHHHLYEKKKTKEG"
BASE COUNT          520 a          524 c          628 g          541 t
ORIGIN      
        1 ccagtgtcag tgtcttcgtc gttatcatgg cggcggggta tcacgtcatc cgtgagtgac
       61 cagcgggtcc tgatctgacg tttacgatcg cgatctggct gaggttcgtc ccatatcagg
      121 cccagcccca gcatgtcccg tcgaagccct tcaatggcct cttccacaca gggttccggg
      181 caggggaggc cgagacggct ggcttcattt tctgcccggg caacggccac cacgccggcc
      241 ccttcgatga agccttttgt ggtgtttgcc cggtgaaggg catagaagag cgtccggtca
      301 acgcccttga gccacagcca gttcggaggc gtcaggcgca aatcatgtgc gtaaagccag
      361 accagaccgc tgcgaacgta acggtgcagc ctgagccact gttgtgcgcc ttctgttttg
      421 atgacccgga taaaggcgtt tctggccagt gaatacaccg gcgtgctgaa acgcccctgg
      481 tcccgtttac ttttgagcct gcaggagaga ttgaggttgt tcatcagggc taccgctgct
      541 ttccggtcac cgaggaagaa ctgcaggcca aagatggcaa agagcgcttt ttcatgtggg
      601 gccatgtctt tccagctggt cagtggttgc cccagttgcg ccatgaagca ctggcgtgtg
      661 gacgcaacat cgagctgcat gttgcggata aggttgttct gctccacaaa gacttcgggg
      721 gttagtgcga cacggcgctc gggacgtttt tgtccgtgga agagtctgtt gctgtctccg
      781 tcagccagaa cgggggcgat cgccggtgac agtgatgaaa acaggtgtgg cagcgtgtgg
      841 atattcagtg gtctgcgggt gaagcggggc tgggccggat gctgaaacca catccacaga
      901 aagccgagcg tcatgggaag cagaatcagc cagaggatcc ccacggtctg tgacatgaca
      961 tccagccact ggctgaaagt gatcgttgca gcgttgttgg cggtgttggc cagcaggtta
     1021 taacgggggg cggcaaaatg gtgaaaaggc ccccagtcag tcatccccca cattgtgtgc
     1081 aggatcaggc aggtggcaaa gacgatgtcg ggtaaaaaga gccagatgag aaacgcaaag
     1141 agcaggcata tggtgccaac gatataccat atattcatcg ggtcttcagt tgtgggtgag
     1201 tgttggtaag acacgagtat tcctccatcg ataaatggat aaatttcggg atgaggctga
     1261 gtggaattaa tgcgaacaat cgccggtaat gaccgggcag ggatgaaata accagggtat
     1321 tttcacagga acagcagaag gcagtgagag acaggcacaa aaaaaccgac tggtgaggtc
     1381 ggttttttgc tattccgggg cggagttggt gactctgacc gaaagggaaa tcactctaca
     1441 tctaacacca tcatagtagg ctgcgcttct cttttttgca atcagaaaaa tgactataat
     1501 cgctccgtta gctctgtctg gtgatgcttc tgccgtgatc tccaatggga cgatgaacgc
     1561 gagattttaa gtcctggctg atcgaacagg tggtgctgtg ttcagtgctc tggctggaga
     1621 gaaatcactt tacatctaac aatgaaagag ctgttgtgac aggcaatgta agagtaaaca
     1681 acagtgattt cctccattga cggagaaacg gatgattaac ctgttaatca tcgttctcag
     1741 ggcggttgtt gctgtggcta acgcagtgat agcaatcctg gaactgatcc gggaacttat
     1801 tgactgataa tcccggaaac gtacttaagg gcagaggggc accgccttct gccctttaat
     1861 aaacaggata ttattacccc cacaggactg ccagtatcaa aaatgcacct gcagtaagca
     1921 tggcaagttt ttcccatatc atgggattgt catccctctt ttgttttctt tttctcatat
     1981 aaatgatggt gcttactcag acggaagagt atttcgtctg tgccagtctt ccggattatc
     2041 ccgctcattt gttcacgcat ccggatacgt ttaatacaga agtcgatttt agcaactgcc
     2101 aggtttttgt tgagggtcag cgtggcagca tgatatgcat gctgccacaa tattaatgcc
     2161 cgggcataat catgatgtat ttccgcatct tcagctgctg ccgttatctg cgc
//
LOCUS       DAZKJR010000309         2212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKJR010000309 DAZKJR010000000
VERSION     DAZKJR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.311"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..701)
                     /gene="tssG"
                     /locus_tag="ACHOWS_005236"
     CDS             complement(<1..701)
                     /gene="tssG"
                     /locus_tag="ACHOWS_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFQ0050649.1"
                     /translation="MERESQPARTGLTQTLALNNDIWRVSFYRFCQLLELENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRVLITEPDPV
                     KVHIDNRSGLGAGKR"
     gene            complement(665..>2212)
                     /gene="tssF"
                     /locus_tag="ACHOWS_005237"
     CDS             complement(665..>2212)
                     /gene="tssF"
                     /locus_tag="ACHOWS_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFQ0050650.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAGSTWFEIDIVLSEAWSSGLPFET
                     ENFRLHCAPVINLFTLEADPLILNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVMNLTAPTMPLYPPANDRFHWRV
                     MSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVAVKHTLIRRFEKGFMLR
                     GVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKEN
                     HNLHVPG"
BASE COUNT          493 a          620 c          639 g          460 t
ORIGIN      
        1 atgcgttttc cggcacccag cccactgcga ttatcgatat ggaccttaac cggatcgggt
       61 tcggtgataa gtacgcgggt atccggtgcc agcagactca ccagagcccg gatgccttcc
      121 gcattgcggg tgggtaatcg catggcaccc agtaaggcca gaaaacggga aaccggcgtt
      181 gccacctgtt cagcggtgcc gggtataccg agtccgacca gccccagcag acactgcgat
      241 gtggcatcgc gtcccccctc ctcaaaagtg gccggatacg catattttcg ccagatccgg
      301 tagtactgcg tggtgatgcg gtggctgaaa atatccagaa atgaggtgag cgcctcatgt
      361 ccttcacggc gctgcgtaat atcatccagc caggacgtcg gcagaggtga gtccacccca
      421 tacattccga gaaatgtcgt gcggattgtt gggggtaatg tcggacggtc ttcgtcgatc
      481 tccaccgctt tcagggtgct gaccgggaat cccatcccgg gccagggtct gaatcgcacc
      541 ggatcatcac ccggatggct ggtcgtgccc agttttggcc cgtccggatt ttccagctcc
      601 agcagctggc agaaacgata aaaactgacc cgccagatat cgttgttaag agccagggtc
      661 tgcgtcagcc cggtacgtgc aggttgtgat tctctttcca tctcagtcgt ttccctgtgg
      721 gttgcagtac cagtgtgagc tggttgaaaa ggtggacatc agcgtagagc gcaaagaaac
      781 ggtgcagcat ctccccgaag agattcacat cgccctcgcc ggcaaaatta tccatattca
      841 gcgtgacctc aatgtccacg ccacggagca taaagccctt ttcaaaacgg cggatcaggg
      901 tgtgcttcac ggcaacgatg gcttcaagcc ggcggcggtt catttcatcg tcggtccagt
      961 catagagcgc cagcgtcccc cgcagcacct ccggattgtc catcatgctg agaaagtttg
     1021 atccgaggtg actcatcacc cgccagtgaa accggtcatt tgccggagga tacagtggca
     1081 tcgtcggtgc ggtgaggttc atgactctga cggggacttt gccggttttg accaccctgt
     1141 ccaggagcgt actttccagc gccctgcgcg gaagctgccc gttggtggcg gtgattcgca
     1201 tcgagaggga ctccggttta tcagacagct gctccagttc gaacgagcgt cccccgagaa
     1261 ctaaccatgt atcatacaga ccggaggggc cccgtttcac gcgggtgtgg taataacgtt
     1321 caggagcatc gtggcgcatc ataccgcccc gatgccggaa gctggtgaac gggacataag
     1381 ggtgtctgcc attcttcacc gagccgtgaa tgttatcgac gctgtaaatt tcggtgtgac
     1441 catcctgaag acgcagcggc ctgagcaggt actcgttatc cagcgggtta aggatcagcg
     1501 ggtcggcttc cagggtaaag agattgataa ccggcgcaca gtgcaggcgg aagttttctg
     1561 tctcaaaagg caaaccggat gaccaggctt cactgagcac aatgtcgatt tcaaaccagg
     1621 tactgcccgc cgtgagcccg atggtgtcga ggccgcgcag ttcaacaaac ataaacttcg
     1681 gacggaagct gaaatactcc agcagcaact ggtaaccact gaaggcagta tcggctttct
     1741 tccacaggca gtcattgtcg tcaaagccca tgggtctgca ccagccgtca aactgatgac
     1801 gtccggtata tgtcccggca tgacgcgcat acatggcatg tacacgccgg gtcattgcca
     1861 gatgcagagc agcgcttacc ggcgcctctg cgttaagaaa aatggcgact ttgtcgatcc
     1921 cggctttact ccagtccact ttttcagggc attcgaagcg caggcggata gcagagcggc
     1981 catcggattc agtatggaga cgggcatctg caaggtgcag cggttgtagc aacacatcgc
     2041 gggtcgtccg gtactgacag gcggttttat gcggcccgac aggacgggac aaaacggaga
     2101 agccttcagc cagtatttcg gcctgacgca gactacgcca gtcaggcgag aactcaacga
     2161 tggccagcga cggaatagtc cgcatatagt gcggccacag caggctcacc ag
//
LOCUS       DAZKJR010000310         2196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKJR010000310 DAZKJR010000000
VERSION     DAZKJR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.312"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..1387)
                     /locus_tag="ACHOWS_005238"
     CDS             complement(104..1387)
                     /locus_tag="ACHOWS_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0050651.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGRNRTRMTILEIHDCRPEE
                     HQWVDQVVNVTMKAWQISGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKVVSDL
                     SEGAVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVKSW
                     TNTQIKAAWTEIQHVTDMTLDMLKQVDWVQVLTNVAKTVGVILVAVGVGGLVVTLGIP
                     EAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVS"
     gene            complement(1387..>2196)
                     /locus_tag="ACHOWS_005239"
     CDS             complement(1387..>2196)
                     /locus_tag="ACHOWS_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFQ0050652.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKLLNEAVLFSAPEGMALT
                     SGEDMQMSAARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGAVDVQ
                     AQNGSMRLFAEKKLTISSESDISFTGKKRITLIGGGSYLRLEAGKVEYGTMATCMRRV
                     KRTMTEAAQSMPMTFPRMSSSHIYSAIYRLLDKHGNILANTPCRLTTPSGQTVMGYSD
                     NEGRSVPVYTRQQEDVELHFFVKKPQPEETMWFVGETDTQQLETELRESQS"
BASE COUNT          450 a          664 c          535 g          547 t
ORIGIN      
        1 gattgactgc cggactgccg tctttgtatt tcacggaggc ttccggcagc catttctcga
       61 tgtagttcag ctcaaaatca tgcaattcca tacctgctcc tgtttaacta actgccagta
      121 ccgccgtacc cgtaccgaga atcgtcgcca gtgttcccca ggcgcttatt gccagacgaa
      181 caatcagtaa aaacgcgcca ataatggctt ccgggatacc cagcgttacg accagtccgc
      241 cgacacccac agcaaccaga ataacgccga ccgttttcgc gacgttggtt aaaacctgta
      301 cccagtccac ctgtttcagc atatcaagcg tcatatcggt gacgtgctgt atttctgtcc
      361 atgcggcctt aatctgggtg ttggtccagg atttcaccga cttaccggtt tggctggccc
      421 agacccagac ctcacttccc tgccggtaaa cccactcacc gctgtcgtta agccagctgg
      481 ctgctgaatt caggaccagt tgcgtctctt ttgacagggc attccagcct tcagccacac
      541 cacgcaccgc cccctcactg aggtcagata ctactttacc agcgtaagtc cagggctcta
      601 ttctggcagc aacgggggtt gatggcggac ggggcataaa gactggcggg taaagagcca
      661 gcggccagta tttcgcgccg ctgatctgcc aggctttcat cgtcacgttg accacctggt
      721 caacccactg gtgctcttcc gggcgacagt cgtgaatctc cagaatggtc atgcgggttc
      781 ggttcctgcc cacaatctgc atatactctt catattggtc ttcatacagc ctatctcccg
      841 gaaacttcac ctccaccagg cgggccaggt tatcgctgtg catattgcct tcggtatccg
      901 gccccgccag tccgggccag cggtcttcct tatttttaac aataatcacg tccgggcggc
      961 ggtaaatgcc cctgccgggc acagggaaag gattggtcgt gtggcgacgg ccagcaccgt
     1021 ctttttccag cagcgtactg ctcagaaaag ggcgtggcgg cgtggtgcgc atatcaaacg
     1081 ccacctccgc cttataatac cagcagtaat tgcgggcgat ttcatccacg cgaataagac
     1141 cactcataac ggtctgctta aggctcatct ggtaagtttt acctttccgg cggatttgtg
     1201 ccgacagttg cggaaaacgc atggcatatt ccacctttat atgcaggtac tgacggtttt
     1261 ccgtggttgg cagacgcgac tgcttttcga ccatttcgac acccgtcgtg gtgcagtccc
     1321 acggctggtt gttcgcccca gtgggaatat cagatgtcgg tgtctcgggt aaatcgggca
     1381 tttccattat gactggctct ccctgagttc ggtttccagt tgctgcgtat cggtttcccc
     1441 cacaaaccac atcgtttctt cgggttgtgg ttttttaaca aaaaaatgca gttccacatc
     1501 ctcctgctgg cgggtgtaaa ccggcacgga gcggccttcg ttatcgctat accccattac
     1561 tgtctgacca gatggagtgg tgaggcgaca gggggtattg gccagaatat tgccgtgttt
     1621 gtccagcaac cgataaatag ccgagtagat atgcgaagaa ctcatacggg ggaatgtcat
     1681 cggcatactc tgcgcagcct ccgtcatcgt ccgcttcacc cgacgcatac aggttgccat
     1741 cgtaccatac tcgaccttcc cggcttcaag ccgcaggtaa ctccccccgc caatcagcgt
     1801 gatgcgtttc ttgccggtaa acgagatatc actttctgac gaaatggtca gcttcttctc
     1861 agcaaacaac cgcatgctgc cgttttgcgc ctgaacatcc actgcccctt ccccggattt
     1921 caggctaagt tgaccggtcc gggcaaaaag ccccagcttc tctccggcca gcgcggtcag
     1981 gttgcccatt gttccggtac tgatatcccc cccggcgttg acagccacat ttcgtgctgc
     2041 actcatctgc atatcttcac cgctggtcag cgccattcct tccggtgccg agaaaagcac
     2101 tgcttcattg agcagtttca gccgctgctc aaacatctgt atctgactgt caatatcagc
     2161 cttcagcgcc tgtgcccgtt ctgcggcatt ttccag
//
LOCUS       DAZKJR010000311         2195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKJR010000311 DAZKJR010000000
VERSION     DAZKJR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.313"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2195
                     /locus_tag="ACHOWS_005240"
     CDS             <1..>2195
                     /locus_tag="ACHOWS_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD1 domain-containing protein"
                     /protein_id="HFQ0050653.1"
                     /translation="MREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFG
                     NYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPG
                     EVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRRIFKDG
                     RYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARAFER
                     YVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRE
                     TDKGVAFYSRKGVTRTPEGNLISDVNRSAAEAKGSPVPQVEAVARGVMSGIKDSDLKV
                     RVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKIRHEIIHH
                     AMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAE
                     KQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPRED
                     GAGASSQYSRSGKPDPFKADAYDAKQFASEVRRIAGLQSVPRGGEVRVGDTPPVLRAL
                     GAPNKELVMPAHVVHKATRPELREHYVSVETMADLPQLLADPVAVMTSKTEHDSLVTL
                     IEAKDRRGAPVIVAIHMRAKGAVAEVNKIASVYGKDNRIALQEQLDTSLLYLNKEKAA
                     DWLLSAGLQLPGANTINGSSGFKVLHPEDIRKGAYYSRTSSLTPEETIAS"
BASE COUNT          534 a          504 c          662 g          495 t
ORIGIN      
        1 atgcgggaga tttcgcgtga ggagcagcgt aacgccacca accgcgaccg taccggacca
       61 gaacgccgta aaggggatgt ttcaccggag cagttcagtg atgcgtttgg tttccgtggt
      121 gtgcagtttg gtaactacgt ggaaggtccg cgtcgtcagg ctgatttgaa ccgggcttat
      181 gactcgctgc atgaccttgc ggaagtactg aatgtaccga caaaagcgct ttccctgaac
      241 ggtcgtcttg gcctggcatt tggtgcccgt ggtaagggta aggcggcggc acactatgag
      301 ccgggtgagg tggcaatcaa cctgacaaaa ggtaacggac cgggtgcgct ggcgcacgaa
      361 tggttccatt ctctggataa ttattttggt cgttatgacg tttccaatga cgggaaaatt
      421 acgtcaggtg gcgactttat gacggaagca cagcgtgtca ggcgcatatt taaagacggc
      481 aggtatgttg atgctgaata tccggtacgt caggaggttt acgacgcttt taaaggtgtg
      541 attcaggcca ttaaaaacag tgacatgccg cgtcgttcag cgcttcttga tgaggtgcgc
      601 tcaaaaccgt actggtcaac ggatgttgaa atggcggcgc gtgcctttga gcgttatgtt
      661 caggataagg cgcgtatggc tggcgtggag aatgattatc tggtcaatat ccgtaaggca
      721 cctgagcaca acacagataa cacctgggct tatccgacga atgcggaact ggatggcggt
      781 attcgtgatg cattcgatca cctgttcagt acgctgaaaa cccgtgagac ggacaagggc
      841 gttgcgtttt attcccgtaa gggcgtaacc cgcacacctg aaggcaatct catttcggat
      901 gttaaccgta gtgcggcgga agccaaaggc agcccggtcc cgcaggttga agcggttgcc
      961 cgtggcgtga tgagcggcat taaggacagt gacctgaagg tccgtgtggt gaagtcacag
     1021 aaagaggctg aagcgctggc gggtgaatcg ttcgacggtt acggcagggt gcacgcgttc
     1081 tatcgtccgg ataaacgtga aattgtcctg gtggcggata acatccctga cgggcggacc
     1141 gtacgcgaga agatacgtca cgagataatt caccatgcca tggagcatgt tgtcacgcca
     1201 gcggaatatc agacgattat caaaaccgtg ctgaaaaccc gcgacagtga taacgccacc
     1261 atccgtgaag cctggcgtaa ggttgatgcg tcctatggta aggaatcacc ggaagtacag
     1321 gcgggtgaat ttctggcaca tatggcggag aaacagccga ataaattcgt ggcggcatgg
     1381 gagcgtgttg ttgccctggt caaaggggta ctgcgtcgta cggggttact gaagccgacg
     1441 gaactgaacg atatcagact tgttcgcgag accatccgta cgttaggcca gcgtgtgcgg
     1501 gaaggttaca cgccgcgtga ggatggcgcg ggcgcatcgt ctcagtactc ccgtagtggt
     1561 aagccggatc cgtttaaggc tgacgcctat gatgcaaagc agtttgcttc agaagtgcgt
     1621 cgcattgccg gattacaatc ggttccacga ggtggagagg tgcgggttgg tgacactccg
     1681 ccagtgttaa gagcacttgg cgcaccaaat aaagagctgg ttatgccagc tcatgtggtt
     1741 cacaaggcca ccagaccaga attacgtgag cactatgttt ctgttgaaac gatggctgac
     1801 ttaccgcaat tgcttgccga cccggttgcg gtgatgacat caaagacgga acatgactca
     1861 ctggtaactt tgattgaggc taaggacaga agaggtgcac ctgtcattgt tgcaattcac
     1921 atgcgtgcta aaggggcggt agctgaggtt aataagattg ccagtgtcta cggaaaggac
     1981 aacaggatcg ctttgcagga acagttggat acttccttgc tgtatctgaa taaagaaaaa
     2041 gccgctgact ggcttctttc agccgggctc caattgcccg gggcgaacac catcaacggc
     2101 tcttctggat ttaaagtact acatcctgaa gatattcgca agggggcgta ttactcccgc
     2161 accagcagtc tgacaccgga agagaccatt gcatc
//
LOCUS       DAZKJR010000312         2193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKJR010000312 DAZKJR010000000
VERSION     DAZKJR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.314"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1238..>2193
                     /gene="subA"
                     /locus_tag="ACHOWS_005241"
     CDS             1238..>2193
                     /gene="subA"
                     /locus_tag="ACHOWS_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFQ0050654.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKRDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGTGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEG"
BASE COUNT          617 a          468 c          500 g          608 t
ORIGIN      
        1 gggggaggat taaccatcga tgattgccgg aacaaaaaac aatataaacc aacaagatag
       61 aaaatcatca caacaaaggg aaaggtctgt ggataagagg gggaggaact ttctgcttca
      121 tgtcacataa ttgatatact ttatcccgca acctccagtt gcattatata taattcaacc
      181 tcaggctccc atcacaggcc aaaaataata tatcgcaagc cgcatcaaat ccggattttt
      241 atataataaa taatacctgt tttttccatc accagtctgt taatcaactt aatatatttc
      301 agggttattt tacattagcc cggcaaaagt tatcaggcat gcaggataca ttatcaggga
      361 gagcggtagt gaacgccggt acctgcttcc ccaaagttat ttaattaacc cgtatcaaca
      421 gggtaacagc atggcaaaat ccagcaatta cgcacctgtt aaggtcatta tttacgttga
      481 tatcggtaaa gatccctgcc acactgttat cattaatcgt tctgataatt agttcgacaa
      541 aatattagca aacgtcgaaa acaagctcag gaaacagata tccggcctca ggaaatatgg
      601 gcatatactg ctgattgctg accagcctgc taccatcgtt gtattacccg tccacattgc
      661 acgctcagaa ggtgtccttg tcagatacct gtaagtggac tgactgcccc gtattgatat
      721 ctattgacca gtcagttcga ctttcccggg gagcagctcg catccccggt ttaacggtca
      781 ttcatgaata tactcagtga cctgatatcc ggggcttacc aatgaaccac accaccattc
      841 actgattgcc ccggtaaaac ggcgctgtga tatcctgttc gccatgatgc ctgacggaac
      901 tttttattgt cataaacatg ccaggcaact tacttgcctc ctcttatttt ttctggttaa
      961 gttttatcca tcatggctgt atttacttct gctctgaagg aggaggcaga gcatcttatc
     1021 gctgacctgt tgcagaaatc cagatgcata ttaccgtgaa ggtgagcgtt aattcattgg
     1081 atacataaat ataaaaatta atgtttttga gacgggcttc actcgcttta ttggttgtga
     1141 ttgtttatct tttgtcgaaa acaggatatt tcagatgtga tgttcctgta agatattgac
     1201 ctgcaatact gtttatttgt actgactgga ggatattgtg cttaagattt tatggccgca
     1261 tattctgttc ctgcttttta tatctgtgtc tgtcagggca gaaaaaccct ggtattttga
     1321 tgcaattggc ctgacagaaa caaccatgtc gctcacagac aagaataccc ctgttgtcgt
     1381 atctgttgtt gattccggag tagcatttgt aggaggcctc agcgacagcg aatttgccaa
     1441 attcagtttt actcaggatg gttcgccatt cccggtaaaa gagtctgaag ctttatatat
     1501 tcatggtact gccatggctt cactcattgc ctcacgtcat ggggtttatg gtgtttatcc
     1561 tcatgcactg atatccagca gaagggttat tcctgacggt gtacaggact catggattag
     1621 agcaactgaa agcattatgt caaatgtttt tcttgcacca ggagaagaga aaatcattaa
     1681 tatatcggga ggccagaagg ggatatcttc cgcatcggtc tggaccgaac tgctttcccg
     1741 tatgggccgg aataatgagc gattaattgt tgcggcagtg ggtaatgatg gcgcagatat
     1801 acgaaaactg agtgctcagc agagaatatg gcctgcggct taccatccgg tcagcagtat
     1861 gaataaaaag cgggatcctg tgataagagt ggcagctctg gcacagtacc ggaaaggaga
     1921 aacacctgta ttgcatggtg gagggatcac cggaagtcgg ttcggaaacg gctgggttga
     1981 tattgctgca ccgggacaga atattacatt cctcagacca gatggaaaaa cggggacagg
     2041 tagtggaacc tcggaagcaa cagctatagt ttccggcgtt ctggcagcga tggtctcatg
     2101 taatccccgt gcaacagcga cagaactgaa gcgtacactg ctggagtctg cagacaaata
     2161 cccctcactt gcagataagg tgacggaagg gag
//
LOCUS       DAZKJR010000313         2189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKJR010000313 DAZKJR010000000
VERSION     DAZKJR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.315"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2189
                     /locus_tag="ACHOWS_005242"
     CDS             <1..>2189
                     /locus_tag="ACHOWS_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD1 domain-containing protein"
                     /protein_id="HFQ0050655.1"
                     /translation="MREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFRGVQFG
                     NYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPG
                     EAAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEARRVKRVFKDG
                     RYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARAFER
                     YVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIREAFDHLFRTLKTRE
                     TDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDLKVR
                     VVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHA
                     MEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEK
                     QPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDG
                     VGASSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVLRHLGA
                     PDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAV
                     DKNGDPVVSAVHMKAVRSRLEINKVASVYGTEGGMSKANSLDKAGLTLYRKKKLSRDN
                     LLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIAS"
BASE COUNT          538 a          499 c          665 g          487 t
ORIGIN      
        1 atgcgggaga tttcgcgtga ggagcagcgc aacgccacca accgcgaccg taccggacca
       61 gaacgccgca aggggaatgt ttcaccggag cagttcagtg atgcgtttgg tttccgtggt
      121 gtgcagtttg gtaattatgt ggaaggtccg cgtcgtcagg ctgatttgaa ccgggcttat
      181 gactcgctgc atgaccttgc ggaagtactg aatgtaccga caaaagcgct ttccctgaac
      241 ggtcgtcttg gcctggcatt tggtgcccgt ggtaagggta aggcggcagc acactatgag
      301 ccgggtgagg cggcaatcaa cctgacaaaa ggtaacggac cgggtgcgct ggcgcacgaa
      361 tggttccatt ctctggataa ttattttggt cgttatgacg tttccactga cgggaaaatt
      421 acgtcaggtg gcgactatat gacggaagcc cggcgtgtta agcgcgtatt taaagacggc
      481 aggtatgttg atgctgaata tccggtacgt caggaggttt acgacgcttt taaaggtgtg
      541 attcaggcca ttaaaaacag tgacatgccg cgtcgttcag cgcttctcga tgaggtgcgc
      601 tcaaaaccgt actggtcaac ggatgttgaa atggcggcac gtgcctttga gcgttatgtt
      661 caggataagg cgcgtatggc tggcgtggag aatgattatc tggtcaatat ccgtaaggca
      721 cctgagcaca acacagataa cacctgggct tatccgacga atgcggaact ggatggcgga
      781 attcgtgagg cattcgatca cctgttccgc accctgaaaa cccgagagac ggacaaggga
      841 gttgcgtttt attcccgtaa gggcgtgacc cgcacacctg aaggtaatct catttcggat
      901 gttaaccgca gtgcggaagc caaaggcagc ccggtcccgc aggttgaagc ggttgcccgt
      961 ggcgtgatga gcggcattaa ggacagtgac ctgaaggtcc gtgtggtgaa gtcacagaaa
     1021 gaggctgaag cgctggcggg cgaatcgttc gatggttacg gcagggtgca cgcattctat
     1081 cgtccggata aacgagaaat tgtcctggtg gcggataaca tccctgacgg gcggaccgtt
     1141 cgcgagaagc tgcgtcacga gataattcac catgccatgg agcacgttgt cacgccagcg
     1201 gaatatcaga cgattattaa gaccgtactg aaaacccgcg acagtgataa cgccaccatc
     1261 cgtgaagcat ggcgtaaggt tgatgcgtcc tatggtaagg aatcaccgga agtacaggcg
     1321 ggtgaatttc tggcgcatat ggcagagaag cagccgaata aattcgtggc ggcatgggag
     1381 cgtgttgttg ctctggtcaa aggggtactg cgtcgtacgg ggttactgaa accgacggaa
     1441 ctgaacgata tcagacttgt tcgcgagacc atccgtacgt taggccagcg tgtgcgggaa
     1501 ggttacacgc cgcgtgagga tggcgtgggc gcatcgtctc agtactcccg tagtggtaag
     1561 cctgatccgt tcaaagtgcc ggaaggtgag ggtgaacgtt atcgcgatga ccttgccaga
     1621 atgatgaaat ctctgcgtac cacagattta acggtaaaca tcgggcgtac gccgccggta
     1681 ttgcgtcacc ttggcgcacc ggatttgccg ctggttatct cccgcgatac tgtgcggaag
     1741 gccaccaatg gtgtgaaaca tgtggtgccg atggatgtta tcgagagact accggaactg
     1801 atgcacgatc cggatgcaat ttaccgttca gcaacagaaa gaaatgcggt tgtgatgctg
     1861 cttgatgccg tggataaaaa tggtgatccg gtagtgtctg cggtgcatat gaaggctgtc
     1921 cggtcgcgtc tggaaatcaa caaggtggct tctgtttacg gtacggaagg aggaatgagt
     1981 aaggcaaata gtctggataa agccggatta acgctttacc ggaagaaaaa attaagccgc
     2041 gataaccttc tgcacagagg gcttcaattg cccaaagggg aacattctta tcgcggctct
     2101 gcggataaaa tactctatcc tgaagatatt cgcaaggggc cgtattactc ccgtaccagc
     2161 agtctgacgc cggaagaaac aattgcatc
//
LOCUS       DAZKJR010000314         2153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKJR010000314 DAZKJR010000000
VERSION     DAZKJR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.316"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..560)
                     /locus_tag="ACHOWS_005243"
     CDS             complement(72..560)
                     /locus_tag="ACHOWS_005243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0050656.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
     gene            complement(566..847)
                     /locus_tag="ACHOWS_005244"
     CDS             complement(566..847)
                     /locus_tag="ACHOWS_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFQ0050657.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIIAAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            complement(1265..1840)
                     /gene="dolP"
                     /locus_tag="ACHOWS_005245"
     CDS             complement(1265..1840)
                     /gene="dolP"
                     /locus_tag="ACHOWS_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFQ0050658.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGAQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPGRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSYIR"
BASE COUNT          557 a          523 c          476 g          597 t
ORIGIN      
        1 tgttgcccgt taacatttaa cttcgtgggc tgacgttgcc acaagcaccg tatttccctg
       61 atttattctg tttattttat tgtggataat gatgccaatg gcatgaataa agttcttccg
      121 gactccggga acgccataaa gccataatgt ttgtaaaatg cgactgccgt gtcatctttt
      181 gcatcaacaa taagcgcata tgctgcaatt tcagatgtgt atgcacgata tagcgcatca
      241 gctaaaagtg ctcccccaag ccccgttcct tttgatgaca gacttactgc cagtcgtccc
      301 atacgaatag ccggaatagt gggatatcgc ggaagctttt ttctcaaatg ctcaggtaag
      361 tcagttaata atatactggc cgctgataac gtatagtatc cgatgatatg atgatcgtta
      421 tctacagcga caaaacaggc cgcgatgcgt cgtcgaatat cctgtgtgac atgctctttt
      481 atgtaacggt ccagagcatc agagccacat aaaaatgagg ttctgtcata tgtgctgtta
      541 aatggtgtca gttgaaacat tatatttatt ttgtgtacag catattatta cggcgtttaa
      601 aggcccgttt aagcgcatca ttcggctctg ttggtgacag taatgcctga gcaaaaattt
      661 cctgatctgt ccgggacagt cggattatat cggtttgttc gatagcgcgt tgggccgcat
      721 cctgaacggc agcaatgata aaatcggtca tagtgcgccc ctggatttct gcagcgcgct
      781 tcagcatggc atgtagttct gtgctgatcc ttgcttccag tcgggcagtt tgtgttgcag
      841 atggcatgat ttgtacctcc tgaatacact gtacggcaaa ttgccgtatt gtgcaaatta
      901 cactctgaca ccgggacggc agacagacat tctgcctgaa aagaaaaatc agattcagac
      961 aaaactatcc gggggatgtt aacgccacga tctttgacca ttgcatacag ataaaagctc
     1021 agattattta ccagcagtga catccggagt acaccgggat tttatctgtg agtgaacata
     1081 cccgggcaaa aaaatccccc gaattcaggg gggtgtcaaa gaggaattat gtatattggt
     1141 tattataatg agcacgtctc cggtattccg cgacgaatgt tcacgtggtg gcatgaacgg
     1201 cccggaaacg tgctcttctg atttttaacg taaatttaca ctaaaggcaa ctctgatact
     1261 gccttcaccg gatataggag aaggccgtcg tgacccgctt cactccgctc accctactgg
     1321 caacatctgc tgccgctcgc ccttcctgct ctgtaaccag gcccagtaaa aagacttcgc
     1381 tgttttctgt caccaccttg acactggatg atttgacacg ggcacttgtg agtaactgag
     1441 aacgaatttt tgtcgtaagc caggcatcac ctgccattat gaccggactg acgggtgtcc
     1501 cctgccggat ttcattatag acctcctgaa caccatcgac accgagtgct atctgacggg
     1561 cacgggcaga taattcacgg cccggagact gaccgatcag cagcacgcgt ccctgatatg
     1621 caatctcact gatgcgtgct tcttttctga tttgttcatc ttttgaaagt gcactgctga
     1681 ccctcagctc cagcacttca tcatccacct gcgctccgac actgcgtggg tcggttgctg
     1741 cctttgtccc gacagcagct gtacccacca cagcggcagc cacgcatccc tgtaacaata
     1801 aggctgaagt aaggacaaca gcagaatata ataaacgcat atctcccccg gaattgtttt
     1861 gatttttact tcatattccc ggaatttatc gtacaaaaag tttaccttca gtgaaacaaa
     1921 acaagctaca gactgttatt atcctccggc acagccggaa atagcggatg cgttacgtgg
     1981 catatctgta tctgtttttt gcacgaaaac tttgataccg tcgtaaagat tcttttttta
     2041 actatctggt tcgctgaaca ggatctcctt ttcgttatac acaggctaaa aggcaccgtg
     2101 gagcccccgg tgcctttttc ataaaacaga gaaaacacgt ctttgaatat acc
//
LOCUS       DAZKJR010000315         2144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKJR010000315 DAZKJR010000000
VERSION     DAZKJR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.317"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..63
                     /locus_tag="ACHOWS_005246"
     CDS             <1..63
                     /locus_tag="ACHOWS_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFQ0050659.1"
                     /translation="IVSSLHEMSNSGRLDDIPEE"
     gene            44..1750
                     /locus_tag="ACHOWS_005247"
     CDS             44..1750
                     /locus_tag="ACHOWS_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFQ0050660.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            1750..>2144
                     /locus_tag="ACHOWS_005248"
     CDS             1750..>2144
                     /locus_tag="ACHOWS_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0050661.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDA"
BASE COUNT          596 a          498 c          560 g          490 t
ORIGIN      
        1 atcgtgtcat cgctgcatga aatgagtaat tcggggagac tggatgacat tccggaagaa
       61 taaaacgcga tgtgatgaac ctgcagaaat gactgagacc gaacaacgtc tgttcattat
      121 gacaaaactg agcaatccct ggtggcgcct caatcatctc tacaaaatac agaacgaaaa
      181 aggtgaactg gtcaccttca gaatgcgacc ggcgcaacgt cagttgtttc gtaacatgca
      241 taataaaaac attatcctga aagcgcgcca gctgggattt tccacagcca ttgatattta
      301 tcttctcgac caggcattat tcattccgca tctcaaatgc gggatcgtcg ctcaggataa
      361 acaggctgcc agtgaaattt tccgcacaaa aattgctgta ccgtttgatc atctccctga
      421 ctggctgaga gcctcattca ccatcgttga acgtcgtagc ggtgccagcg gtggctatat
      481 cctgtttggt cacggctcga gtattcaggt ggcaacctca tttcgctcag gtacggtgca
      541 gcgcctgcat atctcagagc acggcaaaat ttgcgcgaaa tatccggcta aggcgaaaga
      601 gctgcgaacc ggtacgctta atgccgtctc tgatgaatgc attatttttg atgaatccac
      661 ggctgaaggc gtgggtggtg atttttacga gatgagtaac cgtgcacagg agagcactgc
      721 atcaggctta ttgctgacgg cacaggatta taaattccat ttttacgcat ggtggcagga
      781 tcctaaatac agcgccagag tgcctgaaag cgggctgaag ctgtcacggg aaaaaatgac
      841 gtatttttct gcggttgaga aggcaatgaa catcacgctt accgatgagc agaagcagtg
      901 gtacatcagt aaggaaactg aacagcgtga ggaaatgaag caggagtttc cctcaacgcc
      961 acaggaggcg tttctgacgt ccggacgacg tgtgttcagt gccgaaagta cattgcaggc
     1021 agaatcattc tgttcgccac cgatgattgt ttatgacatt gaacctgtta caggagcgaa
     1081 gactaaagct cagtctctgc gtgaaggaaa taaaaacgag ttgcagcgga cgctgatgaa
     1141 ttatctgctg gtatgggaac tgccggatcc ggatgaagag tatgtttgtg gggcagatac
     1201 tgccgaaggg ctggagcacg gagaccgctc atcgctggat gttgtcaaac gcagtaatgg
     1261 cgagcaggtg gctcactggt tcgggcatct cgatgctgaa ctttttgctc atctcatttc
     1321 gcaggtctgt cgtatgtata acaacgcgtt tgtggggccg gagcgtaata atcacggaca
     1381 tgcagttatc ctgaaactcc gggaactcta tccgacacgt tatatctaca acgaacagca
     1441 tcttgaccag gcatatgacg acgatacgcc ccgccttggc tggctgacaa cccgtcagag
     1501 caaacctgtt ctgaccgaag gaatgaaaac gctcctgaat aatggaatat cagggatccg
     1561 ctggtcaggc acattatcgg aaatgaacac ctacgtttat gacgcgaaag gctccatgaa
     1621 tgcacaggaa ggctgctttg atgatcagct catgagctac atgattgccc aggagatgcg
     1681 cgccagaatg ccggtgaggg taaaacagaa aacggataaa cgcagaacca cacactggat
     1741 ggcacactga tgaaaaatga aattaacacc acagcgatga aaaacgatca tggatccacg
     1801 ccgcgttttt ctcagcgtca gttactgtct ctctgttctg atattgacag tcagcctctc
     1861 tggcgtgatg ccgcaaacaa ggcctgtgcg tattatgatg gcgaccagct ggcaccggaa
     1921 gttatccagg tactgaaaga tcgcggtcag cccatgacca tccataacct cattgccccc
     1981 acggtagatg gtgtactggg aatggaggca aaaacaagaa cggacctgat agtgatgtca
     2041 gacgatccga acgatgaaac agagaaactg gcagaggcca ttaatgcgga gtttgctgat
     2101 gcgtgccgtc ttggcaacat gaataaggcc cgctccgatg ccta
//
LOCUS       DAZKJR010000316         2143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKJR010000316 DAZKJR010000000
VERSION     DAZKJR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.318"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1609)
                     /locus_tag="ACHOWS_005249"
     CDS             complement(<1..1609)
                     /locus_tag="ACHOWS_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050662.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGV
                     ATVQDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKATGKAVSDGAKASDEWLTEQM
                     SPGAVRALNTPITEGYDDSAVLLAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTAG
                     LEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENMALRETAG
                     MDVSPWEGVTDAGIEGTVFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAP
                     QPEPVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKE"
     gene            complement(1695..>2143)
                     /locus_tag="ACHOWS_005250"
     CDS             complement(1695..>2143)
                     /locus_tag="ACHOWS_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050663.1"
                     /translation="QVRDRSKFAEISKNYGYFMPKQQGLSLAKLQEVASNVAADAIKN
                     GRDAQAEVDEFYAANGLQPHQQKVQQRKTQQLLQKWQTWAAGDPVKQEFVKEAAANAP
                     ALLEPGREKEVEIYYQNHLRDKKAEHEKALDYSASAAAEKIKGWMK"
BASE COUNT          437 a          664 c          486 g          556 t
ORIGIN      
        1 cctctttctg gctgagtctg cggtattcct caagcagttc agaacgcggc ttcgccttca
       61 gctcattcat cacagcctgt cgcttcgcct gttcgtccag ttcattcaac aactggctgg
      121 cagcttcccg gcgatgagct gcggatgcgt caccctctgt tgccatatcc gcatcagcat
      181 actgttccag aagctgctcg cgattcatgc cctgcatgga ttcacgatgc tgtgccaccg
      241 gatcaaccgg ttcgggttgc ggggctgcgt cgtcctgctg cacggtttca gcatcacgca
      301 tggctgcttc ttctgcggcc tgacgtctgc cacgatatcc ggcaaccgca ccgaatggag
      361 cccccatcgc agcaccgaaa acagtgcctt caatccctgc gtcagtcacc ccctcccatg
      421 gcgatacatc cattccggcg gtctcacgca atgccatgtt ttcctgatag cgcgaatagg
      481 cgttctgtgc cccgttaatt acgccctgtt ccgtggcatt tctgacaatg ccgcttttaa
      541 cggtcttcgc tgtgcctcgt gtcaccagat taaacagttg tgcgtcaccc agttttgccg
      601 ccatggcatt cacagccagc aattcaggat cggttgccag ctgcgcgcgc acctcatcgg
      661 caacacgctc ttttgccaga tccattttct ggcgatcagt aagctgtgca tgctgcgggt
      721 cggcgtcaat ggacaaaaac gtctgctgaa atttcggtga ctgcgccagc tcagagtaat
      781 ccgcattaag aacagcatct gctgccgcca ttgcactctg cccctgtgca ctggctgtgg
      841 aatgggtgat caggcccgcc tggaataaat ccggcatttt tttatcgaca gcttctgctg
      901 ccagtgccgt ggctctttcc ggctgcatcc ctgccgcgat gtattttttc tccagcccgg
      961 cggtcagcat ttttcgcagt gtgacatcac ccacctttct agccacaccg ccagcaacca
     1021 tatcaggtac aagcgcacca atcaggttta cacccttcgc cagcaagacc gcagaatcat
     1081 catagccttc tgttatcggc gtattcagcg cacgaacagc acctggcgac atctgttctg
     1141 tcagccattc atccgaggct ttggcaccat cactgacagc cttaccggtt gctttcagac
     1201 ctttaccgac agtatcagtg accacgtttt ttccatcagg cagggtatcg atgacctcat
     1261 cagcccccct gctgccaccg gcaaaaatat cctgaacagt tgcgacaccc ggtagcccca
     1321 tacggctgaa ctcatttaaa atacgcgccc ctgtttttac cgggctctga atcatcgcat
     1381 caccgagtcc acgggccatt tcccctgttc cccggacgga ctgggcaaca cctttaccca
     1441 ttgttggcaa tacatcaccg aggctgaacg atgtgctgtg atcaatggca ttctgcgggt
     1501 tgctgaaaaa atcctgccag taattttctc ccggctgctt aatgttcagg ctgctgcggt
     1561 tctggttacc caattgcgct tcagggcgct gctcatctga aaaagccata cagactccag
     1621 taaaaaaaag cccgacacga tggtcgggcg taagtgaacg atattaacgg ggagcgcgtt
     1681 actgccgcat atctttactt catccagcct ttgatcttct cggcagctgc ggaagcgcta
     1741 taatccagcg ccttttcatg ctctgccttt ttatcccgga ggtgattctg gtaataaatt
     1801 tccacctcct tttcacgacc aggttccagc aaagcaggcg cattggcggc agcctctttt
     1861 acaaactcct gtttaaccgg atcgcccgcc gcccacgtct gccacttctg taaaagctgt
     1921 tgcgttttcc gttgctgaac tttctgttga tgcggctgta aaccattcgc agcataaaat
     1981 tcatccacct cagcctgagc atcacggcca ttcttgatcg catccgccgc tacgttgctg
     2041 gctacttcct gaagtttcgc cagagaaagt ccctgctgct tcggcataaa ataaccataa
     2101 tttttcgata tctctgcaaa tttgctgcga tccctgacct gag
//
LOCUS       DAZKJR010000317         2129 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKJR010000317 DAZKJR010000000
VERSION     DAZKJR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.319"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            735..943
                     /locus_tag="ACHOWS_005251"
                     /pseudo
     CDS             735..943
                     /locus_tag="ACHOWS_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            930..1119
                     /gene="tnpB"
                     /locus_tag="ACHOWS_005252"
                     /pseudo
     CDS             930..1119
                     /gene="tnpB"
                     /locus_tag="ACHOWS_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1122..1310)
                     /locus_tag="ACHOWS_005253"
     CDS             complement(1122..1310)
                     /locus_tag="ACHOWS_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFQ0050664.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            1845..1986
                     /locus_tag="ACHOWS_005254"
                     /pseudo
     CDS             1845..1986
                     /locus_tag="ACHOWS_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          587 a          502 c          426 g          614 t
ORIGIN      
        1 gcctcacaca gcattaatgc agaaacgaca gagcacacaa ccatacttaa ttttcgcata
       61 aaaaaatccc ctgtgcacat ttttagtatg gttttaaaaa ttaaactatg gcgaaaaaat
      121 tatcactatg caaagatgta acaaaatatt gctcagtgat ctatattgtt gtttttattt
      181 gaggttttat attttctagt gtatttgact tttctttctc aagtgttatt tcttgacctt
      241 tatcacagaa ccatctccct ggatatcccc atcatattgc atgaaaagca agttttacca
      301 atcttgattt tcagtgttaa ataagattaa taactgtaat tctgcaaaaa aaagaacacc
      361 atatgtagta tgactacgta ttccactctg ttcgttcacg gactggcatc gaagtctgag
      421 gtgtactggc aatagcggat acagtcattt gttctttttt taagcattca tccgatgata
      481 cttttcctga atgctaccgg agtggtattc ccctgccgtg agggagggcg ctgacggtta
      541 taaaaatcct gatatatacc cattcccgta tgacagcgat ggcgtcacat ctctgttcta
      601 ccatcttcac agaaatattt cgttcattag cattccggta ttcgtataac caccttcggt
      661 ggagaaagaa taaacaatcc ctggaatgcg atgagcgctt gaccagaaaa gcatcatatt
      721 gcttcatggc gcatcagcga ccacaggatg ccgcaaaagc cgaaaaacgg accagagcgc
      781 ctgcaatcat tcctcgcctc agtatctgtt gcccatggag tggccgataa cagatcattg
      841 tcggatgagc cttatcctct tccttcccgg tggtatccgg atgtgctgcc agccttctca
      901 gttaaccgat gttttccggg ctttttgcca tactgaggac tgataacgta ttcctggtcg
      961 taaaacccgt caatatgcgc cggggcatcg acaaaactga cacagtacgt ccggaaagaa
     1021 cttaacgctg catggcacga caatgctgcc tttgtcttta ccaacaaggc tcgttcccgc
     1081 ataaaagttc tacgccggga taaacatggc gtatggctgc gtcaggcctt cactacttca
     1141 caccggaagt cttccaccct gacacgaata taccgtccgg gctctttcgc aggtgcaaac
     1201 agtttttcac attcggcgaa tgtcatttct gaatcctggt aacgcaacca ctgaaccggc
     1261 atattaccgg gtttgattat tgtcgcagtt attttatatc gtttccccat atcgcctctc
     1321 atcctgacaa caaaattacc ggctgagcca tcactccggt aaagcatgcg aaaaaaagtg
     1381 agcgtctcag caatggctca tgcgatgatt ctgtacgggc accgatggca cctcagaata
     1441 caacaaagat atcagagtaa catgtctctc tcgttatttt tcagagttct tgacaccctc
     1501 cttctgccac attgtcaacg ccagtggaga cttctgctgt cagttttttc atccatacag
     1561 agtgacatta attatcctga acaaaacgaa atatcctgaa tatatttatt ttctcctggt
     1621 gccggagaca tgatgatgta caggctcagg tgcggtgtga atgtaaaatg gtatgttaat
     1681 tattcatttc tgaatgcaaa ctgaatgcaa acttattccg aattgaattc tcctgacacc
     1741 acatgataaa aatgaggata taataaaata cttctcttct ggatgtgcca ttacctgccg
     1801 ttgctgtaat acggcgggta ttttttactg acatatcggg aagccagcag acgttcgtag
     1861 ctacatcccc gaaagtggca cactgcaaac tataatacga gtaaaacatc gatcaccgta
     1921 gctgatgtgt tcaacgatcg tgcactagcg ttctgcgatt ctcagggact gcctgttctg
     1981 agaatcggcg agtccgcgat cggatacgat gcgatttcgc agtgactcag ggattgcatc
     2041 tgaaaaccct ccggctatgt cggtaaatat ttatttctat gaaccgtacg attcagatag
     2101 cattcgcccg gccccgtaag aataatctc
//
LOCUS       DAZKJR010000318         2115 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKJR010000318 DAZKJR010000000
VERSION     DAZKJR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.320"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..482
                     /locus_tag="ACHOWS_005255"
     CDS             81..482
                     /locus_tag="ACHOWS_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050665.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            576..1232
                     /locus_tag="ACHOWS_005256"
     CDS             576..1232
                     /locus_tag="ACHOWS_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050666.1"
                     /translation="MGGSKGGGDTEVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            1235..1681
                     /locus_tag="ACHOWS_005257"
     CDS             1235..1681
                     /locus_tag="ACHOWS_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050667.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNPQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            1691..1963
                     /locus_tag="ACHOWS_005258"
     CDS             1691..1963
                     /locus_tag="ACHOWS_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0050668.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGATIGATVGSAVPVVGTAVGAVAGAVIGGIAGALF"
     gene            1974..>2115
                     /locus_tag="ACHOWS_005259"
     CDS             1974..>2115
                     /locus_tag="ACHOWS_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050669.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQ"
BASE COUNT          538 a          456 c          658 g          463 t
ORIGIN      
        1 gcgttcttac agtgtaagca gtaggaaaac cgcgacgggg acgaaagtcc ccgtcaactg
       61 gttgctgagg ttcagccgat atggcacccg tttcaggtga gagaatgaac gataaaattc
      121 tccggtatat gcagcgtgtg gtgagaaatt cccgcaaccc tgaatttatg aatgaagtta
      181 aagacgcctg ccttaaaaag caggcgtttt gttttgaggc acctgatggc tttctggtgc
      241 tgcgttctgt gctcagtgct gatggtatcc cttatgttct ggtgttgctg ggcgtgtgta
      301 cggggagtaa cagcgttgag cgttatctgc cggaggtgaa gacattaacc cgtctggctg
      361 gcggacgttg ggctgaattt catacggcaa ggcggggatt tatccggctg ggaaaacgtc
      421 tgggctttga gcgaatgccg gatgatgagg atggcttcat ggtgttcagg atagcggtct
      481 gactgccaca gttttcatca ttgtgtttaa acgaacattg caattcacat tctgaccctg
      541 ttccggcagg gttttttatt atccaggggg ccattatggg tggaagtaaa ggcggtggtg
      601 atacagaagt aaaaccaaca gcagcgcaaa tagcacagga agaagtggcc tggaaagggt
      661 ggcaggatta caaaaatatc ctccgcccgg ctgaagataa cttcatggaa aaggtcgatg
      721 acctgaacag tgagcagcag tacgacaata ttgctggcac cacaaatctg gggtatcaga
      781 aacagtttgg cgaagcacgg aaggagctgg cgggtaatct tgctcagtcc ggtgttgatc
      841 cgtccagtgg tcgctttaac gcggtaatga atgcgaacca gagtgatcag gtaaccgggc
      901 agattgacac aaccacacgg gggcaggtat cgcaggcaga taagtatgtt gccgggctac
      961 aggatgttgc tgctctcggt tctggtcaga aggccgatgc gttacagagt tttaactctc
     1021 tggcagacag cagtctggca aaagctaaat cggatgcaca ggcggcgttt acgaaacagc
     1081 aggggcgagc ctctcttgtt ggcgctggtc tgggtgcggc aggtgcatat gcgatgcata
     1141 aggctggcgg tagcggagga agtggcggtg ctaaaacacc tggcactggc gctaatgcca
     1201 ttcagcatca ggctcagaac tggagactgt gattatggag tacggtaaat acgaaacact
     1261 tgcgagatac ggttataccg gagcagcccg ccctcagggg gactggcaga catccgcagc
     1321 gctgacccgc cagcaatacg acgactggcg caccagatat ttaccccgtg tagcaaggct
     1381 ggctgacctt ggggagaaca acagtctgat gaatccacag cttgcacggg tgggaggcct
     1441 tgccacttcc agtctccgta cagcgcagat ggcgcaggat aaccagatgg cgagatacgg
     1501 ggtaagccgc ccggataatc ccgacagtaa tacgctgggg ttacgtaatg ccctggcaat
     1561 agcaggtgcg aaaaatggta tccgtgaagc cgaacaggat cgccagatga atattctgac
     1621 gggggcttct gcaccggcaa gacagaaact gagtgttggc ggacaactgg tggcagcgta
     1681 agggggcaat atgggatacg gtttactgga tattgcaaat cagtcgcggc gtgaggcatt
     1741 acagggaata agtgacgcag acagacgacg tacagagatt gaggcatcaa ataaacaact
     1801 ggctgctcaa cagaaagctc agaaaaaaca gaatatcggt acgggcattg gtacgggggc
     1861 cactatcggg gcgactgttg gttcagcagt accggtagtg gggacagccg tcggcgctgt
     1921 tgctggtgca gtaattggcg gcattgcagg cgctttgttt taaggagtgg tgaatgagcg
     1981 gatttgcaca ggggttactt gccggattca gcactgttga ccaggcaatg acccgtcgta
     2041 aggagcttgg tttgcgagaa gcacagcttg ctcagcaaca gaaaaataac gagcgcgatt
     2101 ttgaatttgc gcagt
//
LOCUS       DAZKJR010000319         2109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKJR010000319 DAZKJR010000000
VERSION     DAZKJR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.321"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..449)
                     /locus_tag="ACHOWS_005260"
     CDS             complement(<1..449)
                     /locus_tag="ACHOWS_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFQ0050670.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMPEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYRCLPDCHHDEAVL"
     gene            complement(449..1138)
                     /gene="tssL"
                     /locus_tag="ACHOWS_005261"
     CDS             complement(449..1138)
                     /gene="tssL"
                     /locus_tag="ACHOWS_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFQ0050671.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCFHRVTALGFQGVYGLKAVSQSQRDE
                     VMKALSERVSLPDGGLSLVVYRMEKHRWRLIRSVWFWTVLAVILTGVIWWGGYLWLQS
                     LLSSQIPELHG"
     gene            complement(1135..>2109)
                     /gene="tssK"
                     /locus_tag="ACHOWS_005262"
     CDS             complement(1135..>2109)
                     /gene="tssK"
                     /locus_tag="ACHOWS_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFQ0050672.1"
                     /translation="VRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHENNDSWKVCP
                     VARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRLMAMRRESNE
                     RLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAGSMLTFSLDH
                     DPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTWKASLHDIRL
                     REEADLWLSVRSDTPAWQVAEKFPALCLAGSPDNVSEIYGAALRGIPLIPVSRVPAAL
                     PVRMENQYFALDMESPAAHEMLEQGVCMFYVPELLGALELELFAVLRS"
BASE COUNT          539 a          629 c          537 g          404 t
ORIGIN      
        1 agaaccgctt catcatgatg acagtctggc aggcagcggt acatcacaga gagctgaccg
       61 acctggcgcg ggaactgcgt ctgtatgctg cggacaacgt cagttaagcg actgacgtca
      121 ccgacccgca gccaccatcc ccgggctgtt ttacgcagcg gaccgtcctg aaacaacgct
      181 tccggcatgt ctccacatac cagtacaaca gggccccggg tgtcggataa tgccggttca
      241 gcatccgtca gggtaacgtc agatgtgagc ttttgctttt cactccggct gtttttaaaa
      301 acactccagc ctgccagaag aaggatcagc agagtagcca gaccgttaaa aacgttgcct
      361 gccgggatga aataccacag caaccacagc aacgtggaaa cggccaccag ggcatgcagg
      421 gctaccggaa tgatgacaga tccacgcatc agccatgcaa ctccggtatt tgtgatgaca
      481 aaagagactg aagccagagg taaccccccc accagataac gccggtaaga atgacggcga
      541 gcactgtcca gaaccagact gagcgtatca gacgccagcg atgtttctcc atccggtaaa
      601 caaccaggga cagaccacca tcaggcagag aaacacgttc agaaagggct ttcatgacct
      661 catcacgctg tgactggctg actgctttca ggccgtacac cccctgaaaa cccagtgctg
      721 tcacccggtg aaagcaggtt aaaacggccg ggtccggtga aggctgacgc agaacctctg
      781 caatgcgatc ccacagtgct tcaccagccc gaagagaacc aaaaaagcgg gcctgaagcg
      841 gcactttacg ccaggccgcc tggccttcgt caacgtccgg ctccccgcct tccccgcttt
      901 tttcaggctt acggctcatc accgtttcgt ccaggagggc acattgtgca taggtaatat
      961 gctccacact ggcatcgtca taaccggcac gctccagcgc ttcccgcaca ctttcgacct
     1021 gtttaacgca tttgttgtaa agcgcagtgc catcgggcgt gagtgcacct tttttcagca
     1081 tggtgacggt aagccatgtc tccatcatca gctggtcgat atcaatatcc tgtctcatga
     1141 gcgcaatacc gcaaacagtt caagttccag ggcaccaagt aactcaggca catagaacat
     1201 acacactccc tgctccagca tttcatgtgc agcgggactt tccatatcaa gtgcgaagta
     1261 ctggttttcc atacgtacag gcaacgcagc cggaaccctg ctcaccggga tcaaagggat
     1321 ccccctgagt gccgcgccat agatctcact gacattatca ggtgaacccg ccagacagag
     1381 tgccggaaat ttctcagcaa cctgccaggc cggagtatcc gagcgcacag acagccagag
     1441 gtccgcctct tcgcgcaggc ggatatcgtg aaggctggcc ttccaggtct gtccatccgg
     1501 gcgggacatt tccagagcaa tgacccggga tggcagacag gcctccagca gacccgttat
     1561 caggtcgaac agcggaagaa acgcaccggc cggatccttg tggtcataac ccggtatggc
     1621 gtcaggatca tgatccaggg aaaaagtcag catactgcct gccagacgag cgagctccgc
     1681 ccacacctgt tcaggtggac gggaaggaaa acgttcgtat tccgtcagta cactggcgtg
     1741 ggagttcagc gcattcagca accagaaaag ggaaacatca gcgacagcaa aatcggcgag
     1801 tctttcgtta ctttcacgac gcatcgccat cagacgctgg cgtcgggagc gtaactgacg
     1861 attgagcaac accagacgtt cgcgcagaac cggacttgcc ccaaataacg ccaccggagg
     1921 aataaaagac ggatcctgtc gccacgcatt ctgaccatct ctgatcaatc ttgctaccgg
     1981 acagactttc catgaatcat tgttttcatg ggcaaaccgg atggtgaaat taaaccgggc
     2041 aacggctatg ggctcttctt cagagccaaa agcatcctgc agcgtgaccc actcttcccg
     2101 atagcgaac
//
LOCUS       DAZKJR010000320         2098 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKJR010000320 DAZKJR010000000
VERSION     DAZKJR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.322"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..497)
                     /locus_tag="ACHOWS_005263"
     CDS             complement(<1..497)
                     /locus_tag="ACHOWS_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0050673.1"
                     /translation="MDIFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYHQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMV"
     gene            complement(497..2077)
                     /locus_tag="ACHOWS_005264"
     CDS             complement(497..2077)
                     /locus_tag="ACHOWS_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0050674.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQNIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYTVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADTPTLDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGMR"
BASE COUNT          654 a          490 c          399 g          555 t
ORIGIN      
        1 aacaccatct ttgcgctttt aatcttaaga tcaaaatctt gccagttgat agcgtagccc
       61 ccctcatttt ttataatcaa agaggctgtc agaatatttc gctcgctatg tttaattaca
      121 tcaaaaaatg cattatagac aggtgtaaaa acgagaatat tatcaccatg cgatgtcaat
      181 aactctattg cctttgcaat agagtacatc acactggggc tataggttat ccattcaggt
      241 ttaatgtctg tttgatattg ttgatgatac cagtttacta tcgcattcag atattcacca
      301 tgttgccaac gactgtaccc taaaatcggg tgttccaggc gtttttttaa tgctctggtg
      361 atagcgtctg gtatcggaag atccatatct gaaatagaga agggtaatac atcttttaca
      421 ccaaatctgt cctcgcaaaa gtcccattgg gtgcaatatg ttcctttacg ggaaatagga
      481 gtattaaaaa tatccatcag cgcatcccca tgtagttatc catatcccta cgcagggcga
      541 tgactttagt gccgataatg acttgtaggg tattttgatc aagtttaaca accgcaatcc
      601 cgccagctct ttttattgca acttcatcaa tcaatcccat atctgcgatt gttaaacgta
      661 aacgagttac gcaattatcc agtgacttaa tattctcttt accaccaagt gccttaacca
      721 tctgctccgc tgcttgcaac tctgatttat caagagttgg ggtatccgct atagccgtat
      781 cttcgtgagc gttcagcgtg acaggactgg actcttcacg tcctacggtc atcaggttaa
      841 atttgagaat ggtccaacgg aacacaaagt aataaatgaa gaaataggcc acttcaacac
      901 acaccgcgat cggccaacct gttcttgtgc cacgcaatac gccaaagcta ataaattgaa
      961 tcagatcgcc agtaaatccc atcttcacgc ccatatatgg caagaccatg ttggcgaatc
     1021 cattcataaa ggcatggaag acaaacagca gcggactgac aaataagaac agaaattcaa
     1081 tcggttcgct gacgccacca ataataaccg taagcacacc ggaaataagc aatgctttga
     1141 tagcctttct gttttgagca tgagcagtgc gatacatggc cagtgcagca ccagccagac
     1201 cgtactgaat catcagtttg ccctgctcca ttttccccgc caaatccagc ggaatgaccg
     1261 cattctcttt gacgtacgcc ataaacatgt tcagggtgcc gtaatactct tcgccgccaa
     1321 tcacagccga accgccgatt ggcgtaaagc ggaacactga cgtcaccaga tggtttaagc
     1381 caaaaggaat cgtcacacgt tcggcaactg tatagaagaa ataaccaaca ggaccggaag
     1441 ttgatatcca gtggccaatg agcatgaaga gattgaaaaa tggcggccag ataaaaggaa
     1501 tgatcagacc aaatccactc atcacaatca gtgtaatgat aggcaccaga cgtgggccgc
     1561 tataaaaacc taatgattct ggaatgcgta aattaacgat ctttttatac atgctggcga
     1621 ctaataaccc agcaacaatt ccccctaaca cgctggtatt gtaggactgg atccccagaa
     1681 tgatgttttg cccatgtgtc gacatttggt cagcaacgac caataagtcg tgctgtttaa
     1741 gataaaagtt cgttcccaga tgcatcgcca taaaaccaat taagccagaa aaagcaccat
     1801 aggctttatc ctctttatct tttaataatc ctaagggaat cgctatcgca aacaatacag
     1861 gtaaattaac aaaggcaaac aaaccaagac taacaatgaa atcaagtatg gttttaatta
     1921 ttggaatagc cagaaatgga attaactttg ccatatcatc actggctaaa ccacttccca
     1981 gccctagcat catgccacat acacttagca gagcaatggg atacataaat gccttcccca
     2041 ggctctgaaa aaaactccag gctttctttt gtttcatgtg ggttatctca tataaatg
//
LOCUS       DAZKJR010000321         2085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKJR010000321 DAZKJR010000000
VERSION     DAZKJR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.323"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            682..1458
                     /locus_tag="ACHOWS_005265"
     CDS             682..1458
                     /locus_tag="ACHOWS_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0050675.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGIFGYIMHRTMPGISFPVFLLNGLIPFFIFNSISNRSVEAIDANQGLFNYRPVKP
                     MDTIIARTLLETLIYIIVYVLLMLIVWMSGEYFEIISLLQLVLVWTLLIIISCSVGLI
                     FMVVGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQYWTYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            1455..>2085
                     /locus_tag="ACHOWS_005266"
     CDS             1455..>2085
                     /locus_tag="ACHOWS_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0050676.1"
                     /translation="MIKIENLTKSYRTSTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDRGKIITNKTISWPIGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     DKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKERHKESSFLMVSHSLNSLKEYCDVVIYIKSFNEVKYYSVVTD"
BASE COUNT          616 a          359 c          458 g          652 t
ORIGIN      
        1 taagagtaag ctattagatg taaattactt tggtcaggtc cttcacagca gaaacagaat
       61 gtcatgaaag ataaatttac gcagtcactt gatcaactgc ttagttttgc gtcttaaagg
      121 attgtaacta tggcaactgt ggagcgaaaa atcattgggc acgggaaaag tgcggtcata
      181 tagaaaagtg ctctctattc agcatctcat ggtttatcag cgaagacaga aaaaaattat
      241 aacgatgcgc aggaatttgt ggaggtttgc taatatagag cgattcgagc atccctcgtt
      301 aaatctctcg aaccgcagta tattcagaca tttttaaaat acgccagatg attacgacag
      361 aaagaacaca acatataatt catgttcgct gtcctcttgc tgaatacgga attgtcttca
      421 ataaaggtgc tacacattat ggtgagagct accacggttt cttgaggatg cagaaaatgg
      481 atttaccgat atcgtgcaat tttgtatcgg tagtatgtcc gactcatgtc tcatggataa
      541 tgaaccatat attataactg cgggtagaaa aatgcgcatc taacgacttt gcctggacgt
      601 gtcgattaac tgttcctggg tttgctactc ttgtcagtca gacgataaaa gtgagcataa
      661 atatatagga ttacatatca agtgaaaaga agtggatttg aagttcagaa ggtgacagta
      721 gaggcattat ttctacgaga aatacgaaca cgttttggta agtttcgtct gggatatctg
      781 tgggcaatcc tggaacccgc tgctcatttg ctggtgctgt taggtatttt tggctatatt
      841 atgcatcgta cgatgccagg tatctcgttc ccggtatttt tacttaatgg tttgattccc
      901 ttttttatct ttaatagtat cagtaatcgg tctgtggaag ctattgatgc aaaccagggg
      961 ctttttaatt atcggccagt aaagcccatg gatacaataa tagccagaac cctgcttgag
     1021 acactaattt acattatagt ttatgtgttg cttatgctta ttgtctggat gtcaggagaa
     1081 tattttgaaa taataagttt attacaactg gtacttgtat ggactcttct gattattatt
     1141 tcatgtagtg tcggattaat atttatggtc gttggtaaaa ctttccctga aatgcaaaag
     1201 attctaccga tatttcttaa accgctgtat ttcatttcct gtatcatgtt ccctctacat
     1261 tcgatcccac agcaatactg gacatatcta ctctggaacc cattagtcca tgtcgtagag
     1321 ttaagtcgtg aggcagttat gccgggctat atcagcgaag gcgtgagttt gaactatctg
     1381 gcaatgttca ctctggtcac cctgttcatc ggtctggcgc tataccgaac gcgtgaagag
     1441 gcaatgctga catcatgatt aagattgaga atctgacgaa gtcatatcgc acgtcaacag
     1501 gtcgacacta tgtgtttaag gatttaaata tcgaactccc ttcaggaaaa agtgttgcct
     1561 ttattggtcg taatggtgca ggtaaatcaa ctttactgag aatgattggc ggtattgacc
     1621 gccccgatag aggaaagatc atcaccaata aaacgatatc atggcccatt gggctcgccg
     1681 gtggattcca gggcagttta accgggcgag aaaacgtaaa atttgttgca cggttatatg
     1741 cgaagccaga agaactgaaa gataaaattg agtttgtcga agaatttgcc gaactcggta
     1801 agtactttga tatgccgatc aaaacttact catctggtat gcgatctcgc ctgggttttg
     1861 gtttaagcat ggcatttaag tttgattatt atattgtcga tgaagtaacg gcagtcggtg
     1921 atgccaggtt taaagaaaaa tgtgcacatt actttaaaga aaggcataaa gagtctagtt
     1981 ttttaatggt ttctcatagt ttaaattctt tgaaagagta ttgcgatgtg gttatatata
     2041 ttaaaagttt taatgaagta aaatactaca gtgtagtaac tgatg
//
LOCUS       DAZKJR010000322         2063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKJR010000322 DAZKJR010000000
VERSION     DAZKJR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.324"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..647
                     /locus_tag="ACHOWS_005267"
     CDS             180..647
                     /locus_tag="ACHOWS_005267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050677.1"
                     /translation="MKGKIIPEKNKIKNLINQERDAIALWIKDGMRLPDIYRLLKEKH
                     PELTFSQNGFVFSLRRDASDLHENALFNRSTVAVFMQKQHSEMTLMVNSGCLLKNVHE
                     ALFSHISYSVFLRYIVKLYPDLHYQAKLNRKGAVCGELHHEENASVYQNDEVC"
     gene            complement(770..1051)
                     /locus_tag="ACHOWS_005268"
     CDS             complement(770..1051)
                     /locus_tag="ACHOWS_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050678.1"
                     /translation="MKTNKKGTTWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
BASE COUNT          559 a          368 c          438 g          698 t
ORIGIN      
        1 tcatggcagc ctcatgtatt tatgtggaga ttatttcatc ggatattatt ttatccagga
       61 atgttgtttt gttaaagtga gtaagtctta tagattatgt ttctttactg aattccgggt
      121 atgtagacaa agctggttct gtatatgtga acttctttgt tgttcctgag ggagataata
      181 tgaaaggcaa gattatacca gaaaaaaata agataaaaaa cttaatcaac caggagcgtg
      241 acgccattgc actctggatt aaggatggaa tgagactgcc agatatttac agattactga
      301 aggaaaaaca cccggaactg acgttttcac agaatggttt tgtattttca ctgagacgtg
      361 atgcaagtga tttacatgaa aatgcgcttt ttaatcgcag tactgttgct gtatttatgc
      421 agaaacaaca ttctgagatg actctcatgg ttaattctgg ttgtttgctg aaaaatgtgc
      481 atgaggcgtt attcagtcat atatcttact ccgtgtttct tcgatatatt gtgaagttat
      541 atccagattt acactatcag gcaaaattaa acaggaaagg ggctgtctgt ggtgaattac
      601 atcatgagga gaatgcttct gtttatcaga atgacgaggt ttgttaaagg tgttttataa
      661 aaatagtggg gtagtgctgg cacagagatg tcttacaggc attctcccgc aaaaaatgaa
      721 cccgttgctt gttatagtgc cttgcttaat acattgcctg aatccggagt tactttttaa
      781 taattctgtt aatggttatt agctcaccta atatttcgaa gttatcaggt gggcagttaa
      841 tccatttgtt agagccagcg atgatttccc tgtcccagcg ttttattctt tcgccatttt
      901 tttctatgca tatcatgtaa tttgatgggg tcattaattc tttggatact gaaagacatt
      961 cccgaaatga tggtatttca agcaaaactt ccgcgcctga attttcacgg taaaaaatgt
     1021 gccatgttgt gcctttttta ttagttttca cgtgcgtatt ctcataggat aaatataaaa
     1081 ccttgtaaca ttcaggaggt aacaggattt attctgtaag aacaacagac agaacaatga
     1141 tatctgtttt atgtatacga tgtagccagt gatttagtac attgctggct atatcacttt
     1201 tcccattttg tgataagtct gctttgaaac gatggtgtcc ctcctcctgt attgtgaaag
     1261 ttgccacatt tttttcttca tatccctgaa catccccgtc gtgccttata taattgactc
     1321 cattaagaag ttgtcagaat aattatttct gattgcaacg gagttaattt cagtgatggt
     1381 aaagatatcc gtggcaccat aacgcatttt tcatcatcag tatagtggta tgcgtgaaca
     1441 tatatggtgg cattttgcct gatgttgtta aggcttttca gcaggtctac agtcaactcc
     1501 atcgaggtat ccttcaggtt gttatcattc gttgatgatg cgatctgttg aaggcgttgg
     1561 cgacaattaa ttctttttct cttctgaaaa atttacggaa tacaaaaaag atctgttttt
     1621 aactgattgt tactttctga agatattctc tgctccattt gctttccggg taagacgtat
     1681 ggctggttat ggtatggtcg gactgcagcc acagggggat gactgtaata cctctgatca
     1741 ccacagtcag tcatctgtaa atgttaaata cctcacagtt cagtcagtgg attattgtcg
     1801 gtcttacggg atatttgtta aaaaaatact gcatggtgaa tccccctgag cggcggggca
     1861 tatcagtatc gtaggtgttt ggtcacatcc cttatctgtg cgggttcagg tgctgatact
     1921 gaactcaccg ggaggcaccc ggcaccatgc tacataacac tccattacgc tacactcgcc
     1981 cctctccgga ggggttttct ctgcaggatg tgaatatgag atgctgcatc attcactgcg
     2041 aatattgatg gtgagaatct tta
//
LOCUS       DAZKJR010000323         2038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKJR010000323 DAZKJR010000000
VERSION     DAZKJR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.325"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1126
                     /locus_tag="ACHOWS_005269"
     CDS             <1..1126
                     /locus_tag="ACHOWS_005269"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0050679.1"
                     /translation="PAIFSFFSGSGFLDLGFENAGYEVVFVNEFHPPFMDAYKHSRKK
                     LGKQPPRFGYAQESIETIEHSVIKHSMKTLKGENRLVGFIGGPPCPDFSVAGKNKGKD
                     GENGKLSQSYIDLIIEMMPDFFLFENVKGLYRTLKHREFFEQIKNELASVGYILHERL
                     INSIEYGAPQDRERIILVGFLDKSLAKNFEWSGQIYPNKEAFQFPWPTTNTYEEDSSM
                     ECPQGIPEELTIEYWFEKNDVTNHPNAKHVFTARAGLARFQSVEEGDDSKKSYKRLHR
                     WRYSPTAAYGNNEVHLHPYKPRRITVAEALAIQSLPKNFELPGDMTLSNMFKTVGNGV
                     PYIAALGIAQSILQHLGYSNEDNSIESGSGDFELTQKYAV"
     gene            1062..>2038
                     /locus_tag="ACHOWS_005270"
     CDS             1062..>2038
                     /locus_tag="ACHOWS_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFQ0050680.1"
                     /translation="MRITASNLVQAISSLPKNTLFNYINDRNSGKIEIVRIQHPEGPI
                     EIKRFDPKKGQTQATAKTESISTQMLWRLANALEENRPVNVERVFGASYNTRSVLESL
                     LAHTPEFYWCRPARLEIMNAQKSIKPGHKHLIFLPDMPHTNGLLIEHQTNIVVSEMSF
                     DVVHQSVDIETIKPTKGMTIEEKRRHAQIQIALVKIGYCLGLDTWVAANDRSLQYNGK
                     AIAQMDGVISSLSDEQVLQSYNDAIKDARLIDCIWFREGRMMPAVMEIEHSTGIKSGL
                     TRMKQFYDHAPRLQDIRWTIVAPDEYRAKVIEFANMPQFIELKAKFFPYS"
BASE COUNT          672 a          401 c          419 g          546 t
ORIGIN      
        1 acccgctata ttttcattct tttcaggatc aggttttctt gacctaggct tcgaaaatgc
       61 cgggtatgag gtggttttcg tcaatgaatt ccatcctccc ttcatggacg cgtataaaca
      121 ctcaagaaaa aaacttggca aacagccgcc acgctttggg tatgcccaag aaagcattga
      181 aactattgag cattcagtga ttaagcatag catgaaaaca ctgaaaggtg aaaaccgcct
      241 agtgggcttc attggcggac caccatgccc tgacttttct gttgctggca aaaacaaagg
      301 caaagatggt gaaaatggca aattatcaca aagttacatt gacctgataa ttgaaatgat
      361 gcctgatttc ttcctttttg aaaacgttaa aggcttatac cggactctta aacatagaga
      421 atttttcgaa caaattaaga atgaactagc ttcagtagga tatatcctgc atgaaagatt
      481 gattaattca attgaatacg gtgcgcctca agatcgagaa aggattattt tagttggttt
      541 tcttgataag tctttagcca aaaacttcga atggagtggc caaatatacc ccaataagga
      601 agctttccag ttcccttggc caacaacaaa cacatacgaa gaagactcaa gtatggaatg
      661 cccgcaaggt attccagaag aacttactat agaatactgg ttcgagaaaa acgatgttac
      721 caatcatcca aacgctaaac atgtatttac ggcccgagcc ggtttagcaa gattccagtc
      781 agtagaagaa ggcgacgata gtaaaaaatc atataaaagg cttcatcgct ggagatactc
      841 tccaacagca gcatatggta ataatgaagt tcaccttcac ccatacaaac ctcgtcgtat
      901 tacggtcgct gaagcattgg caatccagtc tttgccaaaa aattttgagc taccagggga
      961 tatgactttg tcgaatatgt ttaaaacagt cggaaacgga gttccctata tcgccgcgct
     1021 cggtattgcc caatcaattt tgcaacattt aggatacagt aatgaggata acagcatcga
     1081 atctggttca ggcgatttcg agcttaccca aaaatacgct gtttaactat ataaacgata
     1141 ggaatagcgg gaagattgag attgtacgta tccaacatcc cgaaggtcct atcgaaataa
     1201 agagatttga tcctaagaaa ggtcaaactc aagcaacagc taaaacagag agtatttcga
     1261 ctcaaatgct ttggcgactt gcaaatgcac tcgaagaaaa cagacctgtc aacgttgaaa
     1321 gggttttcgg tgccagttac aacacccgct cggtattgga gagcttgctc gcacatactc
     1381 cagagttcta ttggtgtagg ccagccagac tggaaatcat gaatgcacaa aagtctataa
     1441 aaccaggaca taagcatctg atttttctgc cagatatgcc acatactaat ggtttgttaa
     1501 ttgagcacca gactaatatt gttgtctctg aaatgagctt cgatgttgtt caccaaagcg
     1561 ttgatattga gacaattaaa ccgactaaag gcatgactat tgaagaaaaa agacgtcatg
     1621 ctcagataca aattgcctta gtaaaaatag gttattgttt aggtttagat acttgggtag
     1681 cagccaatga tcgtagctta caatataatg gtaaagctat agcgcaaatg gacggtgtaa
     1741 taagttcatt atctgatgag caagtactcc aatcgtataa tgatgcgatt aaagatgctc
     1801 gtcttataga ctgcatttgg tttagggaag gccgcatgat gccagcagta atggagattg
     1861 aacacagtac tggtataaaa agtggtctga ctcgcatgaa acaattctat gatcatgcac
     1921 cgagattaca agatatccgt tggactatcg ttgctcctga tgaatatcga gcaaaggtga
     1981 tagaatttgc aaacatgccc caattcattg aattaaaagc taaattcttc ccttactc
//
LOCUS       DAZKJR010000324         1904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKJR010000324 DAZKJR010000000
VERSION     DAZKJR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.326"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1183..1785
                     /gene="fimB"
                     /locus_tag="ACHOWS_005271"
     CDS             1183..1785
                     /gene="fimB"
                     /locus_tag="ACHOWS_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFQ0050681.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          577 a          337 c          401 g          589 t
ORIGIN      
        1 ttacccagag tcataaaata gagtgtctgt tgatctcatt ttcatattcc tttcagactg
       61 aagtgcgttt tcatcacccg atgagaaaga actgaagtga tgtttgccat agattgcgag
      121 atgccctgtc tcattgacta tcgcggtgca cctttatacc tgttatacca gatcaaaaat
      181 cacgcaatcc atacaacaaa acgagatttg caattcgtgt cacaaaatat gtcgatcttt
      241 ttctaagagg cagatgccat gtgaagccag acgaacactt gcggtggtct tcaaaaatta
      301 aagattttat tttaactgtc tgttttataa aaaatttatt tagaatctaa acaaagaaag
      361 ctttgcaagc taactcagtg agcttggtga aaataagtgt ttacccgcca tcaggctgag
      421 cataattctc atcatgaaat atgtttcttg gtttgtggct tgtaactggt cactcctgat
      481 gtcgatctgg agaggcttgt tgatgttggt gttttcagga tgatgtttca cttagtttgt
      541 ttgccgtatc gcccggcgaa tagctgtgat tgaggaaggt ttaagtcgta gtgaccaaag
      601 ccatagttac caacggatgt agatgaaaaa gcgtcgcctg cgttccccca tatctccagg
      661 ataaaaggga atgtaacaat ctcatggcct aagctgacga accaggcagg aataatcgct
      721 agggatctaa gaattagcat gataatagcc actaagaaat tactgcgttc catgaaatag
      781 ccattttgtg gcaaatgcag ttgactaata atgtcatatg tgagacggct agttgaacga
      841 atattaaatt ttgctgaatt ttttatgttg attttacttg ttacagaaca tatcacatga
      901 tatatagata agattagttg cattaatgat gagggttatt attcgattcg tatcagattg
      961 ataaatatat aaaggtacat agcatgcaag agcatggcgt ttgtatggca aagttattat
     1021 aagtaactat cgctactcca tttaagttcg ctcagaagaa ctggtccact tacgttagtt
     1081 attaagcaaa cgttcgcttt tataaacata atcaggataa aaatgttgga ttattgctaa
     1141 cccagcacag ctagtgcgcg tctgtaatta taagggaaaa cgatgaagaa taaggctgat
     1201 aacaaaaaaa ggaacttcct gacccatagt gaaatcgaat cactccttaa agcagcaaat
     1261 accgggcctc atgcagcacg taattattgt ctgactttgc tttgttttat tcatggtttc
     1321 cgggcgagtg aaatttgccg attgaggatt tcggatattg atcttaaggc aaagtgtata
     1381 tatatccatc gattaaaaaa aggcttttca acaacgcacc cgctattgaa caaagaaatt
     1441 caggctttaa aaaactggtt gagtatccgt acttcttatc cgcatgctga gagcgagtgg
     1501 gtattcttat cacgtaaggg gaatccactt tctcggcaac agttttacca tattatctcg
     1561 acttccggtg gtaatgccgg gttgtcactg gagattcatc cgcacatgtt acgccattcg
     1621 tgtggttttg ctttggcgaa tatgggaata gatacgcgac ttatccagga ttatcttggg
     1681 catcgcaata ttcgtcatac tgtctggtat accgccagca atgcagggcg tttttacggc
     1741 atctgggata gagccagagg gagacagcgt cacgctgttt tatagagata gttgagatac
     1801 cagggatggt gttaaaaact acattattct tacgatatat caaaaatgat taaccattta
     1861 ttaattgatg ttattgtaag tgtgattctt aattatcata ataa
//
LOCUS       DAZKJR010000325         1888 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKJR010000325 DAZKJR010000000
VERSION     DAZKJR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.327"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1767)
                     /gene="tssD"
                     /locus_tag="ACHOWS_005272"
     CDS             complement(<1..1767)
                     /gene="tssD"
                     /locus_tag="ACHOWS_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFQ0050682.1"
                     /translation="MSDIVYLRIIGEKQGYISSGCGTYASVGNCWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECITVDYDYILCKHLIVNTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKEKGCTDAGTAEEPAENFGQVA
                     IFAQPVVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLDQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDLRKLPGVVTGRGVPLSLGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEF
                     REALWLEVSKDSVLLAQFIKSNQNNVSQGYSPYVPEEGYYYGPNEIVKKFQIHHVVAI
                     EHGGGVYDIDNFRIVTPRLHDEI"
BASE COUNT          502 a          469 c          413 g          504 t
ORIGIN      
        1 aatttcatca tgtagtcggg gcgtaacaat cctaaaatta tcaatatcat acactccacc
       61 accatgttct atagcaacta catgatgtat ctggaatttc ttaactatct catttggtcc
      121 gtaataatac ccctcctcag ggacataggg ggaatatcct tgactaacgt tattctgatt
      181 actttttata aactgcgcca ataacactga gtctttactc acctccagcc acagcgcctc
      241 gcgaaactca tcaaaggttt taaactcccg tccgcgcaat ttatcagcaa tatgcgccgg
      301 gattggtgcg ccgttgccgt tattggatac cgacatatcc agccagcgag ttcctagtga
      361 aagcgggacg ccgcgccctg ttacaacacc aggcagcttt cgcagatcct ccttcaggta
      421 aacatagatg ggcttaatac cggatcccgc cggaaaaacc agaatataat cgttgaattc
      481 tttcggatca ggaaccggga atggcggcgt ggtataggta tcggtgccgt ccggaatcgg
      541 cgtcaccaga atggtgggct gatccagcgg cggcctgtcg ttaccggtat ggcccggaac
      601 attcccatgt tccggatttt ccggcgtcca ggtaatcagg gggccatcga catcgtgagc
      661 gggggtaaat tcataacgct gcgtctgctc gttccactgc atgttcgcca cgcggacgtt
      721 ttcatacggc gtaccttcac ccgtatgaac accatacacc tgaggcttgc cgtgaatgtc
      781 agttccccag aaaaaacgta cccgggtggt ggcggtactg cctgctagtg ctttctgccg
      841 cagttcttcc tcataccaga actcatcacg tccgggcacg cgagcactgc cctcgcccgc
      901 agagggaata tacaaagcag atgccaacgc accgatgtaa gagccgcggg ttgcactgag
      961 tatgccactg agcgaccatt ttccccatat ttgagccgcc aacacactac ccggaaacag
     1021 tagcatggtt tgagcttctg cgggttgccc gacatcgtta gcctcgggat ggtggtatcc
     1081 acagcagtca tcaaccactg gctgagcaaa aatcgctact tgaccgaagt tttcagcggg
     1141 ttcttctgcc gttccagcat ctgtacatcc cttctccttc agacaggatt ttgcatagac
     1201 cgggttaacc gctggtgaag agagtggaag gttaacaact ggtagaggtt caggcttaag
     1261 attactggca ggtgggcttt taggcggaat aaataagcta ttatagccag caggaaaggc
     1321 caaccagtca aattccgtat tgactattag atgtttacat aatatgtagt cataatcgac
     1381 agttatacat tcggtaggaa gattattgtc agaaatgttt acatgaatag cagttaatga
     1441 agcatttctt agctgaatat aataataccg ttcccagcgc ccgcttttat ttatacgata
     1501 caaatcgatt tcaataaata accgctcatt ctgattgatg gcattagaca gtagcggtga
     1561 gcttttatca atgagtttgc aaaattgtag cccctgtaga ttaacgcctt taccagtact
     1621 ggtaatggcg ttggtgagtg caaaggcaaa aatttcatct tcatggccaa cctgccagca
     1681 attaccaacc gacgcatacg tcccacagcc tgaagaaata tagccctgct tctcaccgat
     1741 tattctcagg taaacaatat cgctcacttt atttcattcc agtttatcct taacttattt
     1801 gcattaaata gaaaacttaa ttctcgatca ataagaaacg cttttaatga aagattattt
     1861 caattactta acgttttcac ttagctcg
//
LOCUS       DAZKJR010000326         1878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKJR010000326 DAZKJR010000000
VERSION     DAZKJR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.328"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1736
                     /locus_tag="ACHOWS_005273"
     CDS             <1..1736
                     /locus_tag="ACHOWS_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050683.1"
                     /translation="PADSNLNATGSNKRKYGLVTLKGLPEKNSSTLTPGQRYWVVVDN
                     NNVKPAPGAGPGWWRHLLPPAKEAMVFDKTVSLSSPFAIKVGDPIGHMGYYQAPKDGG
                     YEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLWLKYAPGLALYKKDAFTGTFTKDA
                     RVTTRTGILPLKQVQTEVDKSTKQEYWQLRPENAYVPKGQVEPQLLSQYDLAKLGFRT
                     EIAEPTSFDYLDGKKQPTGFFRSLINSLYEAANDDTRTSHALVKHNYQRLLDKIDSGS
                     DRYSPMEYWRALHNPDYRDVLQKTIVKHPSDWYFKKDDAIWQPFLNALKKDAPEWKKY
                     SEDFLDKMAWMQDVTTEKLGPLLWHMHPIMFLGCMKKSSGIWPLGKTSERYESGGRGP
                     GVISSGKGDHGGVSYGMYQLSSKMGTVNKYIIMSKYKDLFEGKKPATPEFNEIWKKIA
                     LIHKEEFAQDQHLFIKKTHYQVQLDYLASKGLNFSHSLAAINDMIWSTSVQYGPTTGL
                     ISRALKGKNLETLTDADVITIVQDYKINHVEDLFPSSPTWWDDLKTRALTEKKDLLEL
                     NELNLELKNED"
BASE COUNT          609 a          373 c          432 g          464 t
ORIGIN      
        1 acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac ggtcttgtga
       61 cgctaaaggg tttaccagaa aaaaacagca gtactctcac tcccggtcag cgctactggg
      121 ttgttgtgga taacaacaat gttaagccag cgccgggggc cgggcccggc tggtggcgac
      181 acttgctgcc tcccgcaaag gaagccatgg tattcgataa aacagtaagc ctcagttctc
      241 catttgccat taaggtcggt gacccgattg ggcacatggg ctactatcag gccccaaagg
      301 atggggggta tgaggcacga tatcaggtac atatcgaatg cacgagtatg gatgataatc
      361 tggagaaatt cctgaccaat cctgagcagg tgggaaaaaa taatccactc tggctgaagt
      421 acgccccggg gcttgcgttg tacaagaaag atgcttttac aggaacattc acgaaggacg
      481 cgagagtgac gaccaggacg ggaattctgc cactgaaaca ggtgcaaaca gaagtcgata
      541 agtcgaccaa acaggaatac tggcaattgc ggccagaaaa cgcgtacgtg ccaaaaggtc
      601 aggtggaacc tcaacttttg tcacagtatg atctggcaaa gctggggttc agaactgaaa
      661 tcgcagagcc taccagcttt gattacctgg atggaaaaaa acagcccact ggattcttcc
      721 gcagtcttat caactcactc tatgaggctg cgaacgatga tacgcgaacc agtcatgcgc
      781 tggtgaaaca taattatcag cgcctgctgg ataaaattga tagtggaagc gaccgttact
      841 ctccaatgga atactggaga gcacttcata accccgacta tcgtgatgtt cttcagaaaa
      901 caatcgttaa acatcccagc gactggtact tcaaaaaaga cgatgcaatc tggcaaccat
      961 ttctgaatgc gctgaaaaaa gatgctccgg aatggaagaa atacagcgaa gattttcttg
     1021 ataagatggc ctggatgcag gatgtgacca cggagaaact gggccctttg ttgtggcata
     1081 tgcatccgat tatgttctta ggctgtatga aaaaatcgtc aggaatatgg ccacttggga
     1141 aaacttcaga gcgttatgag tctggaggaa gagggccagg tgtaatttca tctggaaaag
     1201 gagatcatgg cggtgtgtca tatggaatgt atcaattatc ttctaagatg ggaacggtta
     1261 ataaatatat tataatgtca aaatacaaag atttatttga gggtaaaaaa cctgctacac
     1321 ctgaattcaa tgaaatatgg aaaaaaattg ctctcattca caaagaagaa ttcgcacaag
     1381 atcagcatct ttttataaaa aaaacacact atcaagtaca attagactat ttggcatcca
     1441 aagggctaaa tttctctcat tctcttgccg caatcaatga tatgatttgg tcgacttccg
     1501 ttcaatatgg ccctacgact ggtttaattt cgagagcgtt gaaaggaaag aatttagaaa
     1561 cattaacaga tgcagatgtg ataactattg ttcaggacta caaaataaat catgttgaag
     1621 atctttttcc atcatccccg acttggtggg atgatttaaa gacacgagcg ctaacagaaa
     1681 agaaagattt attggaatta aatgaattga atctggagtt aaagaatgaa gattaaatta
     1741 tttgcattgc ttattttctt gccgattatc tcttttgcga aagaaccaca cgtttgcaat
     1801 tcctcgatgc agtctcatcc agatattatc gattgcatac aaattagcac aaggaaaata
     1861 gaggattcca ttaaagaa
//
LOCUS       DAZKJR010000327         1860 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKJR010000327 DAZKJR010000000
VERSION     DAZKJR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.329"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..284
                     /locus_tag="ACHOWS_005274"
     CDS             <1..284
                     /locus_tag="ACHOWS_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0050684.1"
                     /translation="DKQFAVILESEAFKELGIVNYSMIPDTELFRYLSDMGKDINSVL
                     NDDRDFRTLMRETDDPDILELLSTERAIKRLATGINKELDIAFNHLEII"
     gene            294..923
                     /locus_tag="ACHOWS_005275"
     CDS             294..923
                     /locus_tag="ACHOWS_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050685.1"
                     /translation="MSGNATNEIILAKINDILVDFNELNRQINNFPEKFSGKIDAINF
                     AIKQTPDALEESLKKLFSAAEELEKTVQESTDECRVSMRAYAINELDNLKVKISEAIN
                     ESIKNSIDMPLDQAKERIIQINKMLQKSTGVEMSTRVAFGIGGLLGLSFLSLAMCISA
                     GYLYFQERSKAERYYNAFREQQIVIERLPAETQRQFRDELGRYLARNQK"
     gene            1264..1623
                     /locus_tag="ACHOWS_005276"
     CDS             1264..1623
                     /locus_tag="ACHOWS_005276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFQ0050686.1"
                     /translation="MVTKKKEKDKVNRDVVISLRLTEKEFSDFVDDIKKSQLSRSAFF
                     RKLILERSSSVVIKVNNTAPLVYHFNKAGNNLNQLAYKVNSAFLMGRISESLYVRVLN
                     ALVDIRELLMAGIKNAH"
     gene            1613..>1860
                     /locus_tag="ACHOWS_005277"
     CDS             1613..>1860
                     /locus_tag="ACHOWS_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFQ0050687.1"
                     /translation="MLIRVKGYNDGVKEYLEEGKKNGRDLSRNELDERLILHGDLELT
                     QQIYRSIPDHGQDRYLSITHSYFEDNIEPDVFLNVINE"
BASE COUNT          630 a          257 c          401 g          572 t
ORIGIN      
        1 ttgataaaca gttcgctgtg atactggaga gtgaagcatt taaagaacta ggtatagtaa
       61 attattcaat gattcctgat acggagttat ttagatatct ttctgatatg gggaaagata
      121 taaactctgt tctaaatgat gacagggatt ttagaacatt aatgagggaa actgatgatc
      181 cagatatact tgagttattg tcaacggaaa gagctataaa gcgtcttgct acagggataa
      241 ataaagagct ggatattgca tttaatcatc tcgaaattat ataaggtgta aatatgtctg
      301 gaaatgcaac aaatgaaatt attttggcaa aaataaacga tattcttgtc gactttaatg
      361 agttaaacag gcaaataaat aactttcctg aaaagttctc agggaaaata gatgcaatta
      421 attttgccat aaaacagaca cctgatgcgc ttgaggaatc attaaaaaaa ttgttttctg
      481 cggcagaaga gttagaaaaa acagtgcagg aaagtacaga tgaatgtcgg gtatcaatga
      541 gagcgtatgc tataaatgag ttagataatc tgaaagtaaa gatatcagaa gctataaatg
      601 aatcaattaa aaattctatt gatatgccgt tagatcaggc gaaagaacgg attatccaga
      661 taaataagat gctacaaaaa agtaccgggg tagaaatgag tacaagagta gcatttggta
      721 ttggtggatt gctggggctt tcatttcttt ctttagcaat gtgtatatca gcaggatatt
      781 tgtattttca ggagcggagc aaagcagagc gttattataa tgccttccgt gaacaacaga
      841 tagtgataga acgtttgcca gcagaaacgc agcgccagtt ccgggatgaa ttggggcgtt
      901 atcttgcccg taatcagaag taaagtaggc cctagttagg ccctagttag gtgtacctta
      961 ggttaattat accttagtta taccttagtt atatgttaat tatatcttag ttagctacac
     1021 cttgagttag ttatacgtta attatacctt agttatacgt taattatatc ttagttatac
     1081 cttaattatg cgtcagttac agaggcgttt tgagggtaaa ttctggaggc acgttaccga
     1141 ctgtatacaa aatgctggcg cattttgata cgtcgacgtg ccaaagggga cgcccctttg
     1201 aaaccccgtc aggagacgga cgacctgacg gtggcattgt taattattta caggaggtat
     1261 cctgtggtca ctaaaaaaaa ggaaaaggac aaagttaaca gggatgttgt tatatcctta
     1321 agattgacag agaaagaatt tagcgatttt gtcgatgata taaaaaaatc tcaactcagt
     1381 cgttctgcat ttttccgaaa actgattctt gaaagatcat cctctgtcgt aattaaagtt
     1441 aataatacag ctcctttagt atatcacttt aataaagcag gtaataatct taaccagctt
     1501 gcttataagg tgaactcggc ttttttgatg gggcgtattt ctgaatcttt gtatgtgaga
     1561 gtattaaatg ctcttgttga cattagggaa ttattaatgg caggaataaa aaatgctcat
     1621 tagagtgaaa ggttataatg atggagttaa agaatatcta gaggagggga aaaaaaatgg
     1681 aagagattta tcgcgtaatg aattagatga gaggcttatt cttcatggtg atcttgagct
     1741 tactcagcaa atttatagat cgataccaga tcatgggcag gatagatatt tatcaataac
     1801 acattcttat tttgaggata acattgagcc tgatgttttt ctaaatgtta ttaatgagta
//
LOCUS       DAZKJR010000328         1837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKJR010000328 DAZKJR010000000
VERSION     DAZKJR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.330"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(197..1621)
                     /locus_tag="ACHOWS_005278"
     CDS             complement(197..1621)
                     /locus_tag="ACHOWS_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0050688.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIVSSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     LAVSLMWIANFLISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPETK
                     GIPLTQIEQLAGNKLREIQGKAMM"
BASE COUNT          644 a          455 c          291 g          447 t
ORIGIN      
        1 aattaatgaa atttaacaca aataccaaat aaaatacgtg tatcaaatgc aatcagaaat
       61 atattttata gttggttata aaaaaccttc tggatattta ttatctcctt ttgttaccta
      121 tattaacaca aggaaaatcc cggtaagatg ctcaacatta ccgggcatta ctgctgattg
      181 tgacacaatc agactattac atcatagctt taccctgaat ttcacgcagt ttatttccgg
      241 caagctgctc aatttgtgtt agcgggatcc cctttgtttc aggaacatag cgagaaataa
      301 aaacataaca aaccagatta aagacaacaa aaatccacat agaaaacgct ccgccaaagg
      361 tttcctgcag ccaggtatta tcatttatta ccggaaacaa cagtgatatt agaaaattcg
      421 caatccacat taaactaacc gccagcccca tgccgaatcc tttaatcttt tcgggaaaca
      481 cctcagaaat cagtacccat gctcctacgc tccagctgac agcaaacaac agcataaaaa
      541 ataaaatccc aaaaatggtg atatagccag tatcatgggt atacaaacca tatgatgcga
      601 tcaacaaacc aataattgac ccgatagtgc caatcttcat gattggtatt cgtccatagc
      661 gatcgaacaa aatcatcccg ataaaactac cgatcacatt acaaacagca ataaaaatcg
      721 tctggaacat cacttcctct gtactgctgt tttgtagcac cagaggtgcg tagtagttca
      781 tcacattgac accagtaaac tgttgtaaaa cagcgataca acagccaacc agcagaatat
      841 agcgcaacac aggagagctt ttcaggataa cgaattccct gcctgcagca ttctcccggg
      901 cctccgtttc cctcatacta tcaaaaatag cggctacatc ggtagcgcta accccccggt
      961 agattctggc aagcactttc agagaacgtt tttccccctt gttattacgg gcacaccatg
     1021 ccggcgattc aggcaaaaat aaagttatca ctaacattgt aacagaaggt atcacctgag
     1081 cgaataacat gctcttccag ccttcttcga ccagcacatc agagggcatc cccttagcaa
     1141 tcacataatt aacaataaaa acaatcacct gaccagaaac tactgatata ttgtaaatac
     1201 ccagagcttt accacgaatg tcagctggtg ccagttcaga catatacatg gtagatgcag
     1261 tccccgccat cccaacagca aaaccactga cgatgcgcga aatcgagaaa accataaaag
     1321 attcactcat cgccccccac agtgacgaga cgataaaaat aactgcaata atcagaagcg
     1381 caggcttacg ccccaactta tcggcaattt tccccccgac cagcgcacca atgatacaac
     1441 caatcacaat gctcgatact gcccacccag tttcagttga agtgagctgg taatattgct
     1501 gtaaaggcag tatcaccccc gttatcactg ccgtcgaata gccaaacatc aatccagcca
     1561 gtgcagcaac gcagcacaat gcgtacacat aggctgaatc atacttgctt ctctgaaaca
     1621 taccccctcc ctgcctgttg aggaaatgac atacgaacat ttattccata cttaaacaaa
     1681 tcagctcaaa tattccacaa caaagatcac aaaatataaa aatttcataa ctgtgaaaaa
     1741 tagtttgtag cacaataatc atattgaaaa tacagaacaa taaaaaacta ttatttttca
     1801 tattgataaa agttacctgt tgatgagtga gtatata
//
LOCUS       DAZKJR010000329         1825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKJR010000329 DAZKJR010000000
VERSION     DAZKJR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.331"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1640
                     /locus_tag="ACHOWS_005279"
     CDS             <1..1640
                     /locus_tag="ACHOWS_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFQ0050689.1"
                     /translation="PADSNLNATGSNKRKYGLVTLKGLPEKTSSTLTPGQRYWVVVDN
                     NNVKPAAGAGPGWWRHLLPPAKEVMVFDKTVSLRSPFAIKAGDPIGHMGYYQAPKDGG
                     YEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLWLKYAPGLALYKKDVSTGTFTKDT
                     GVTVRTGILPLKQVQTEVDKSTKQEYWQLRPENAYVPKGQVEPQLLSQYDLAKLGFRT
                     EIAEPTSFDYLDGKKQPTGFFRSLINSLYEAAIDDSRTSHALVKHNYQRLLDKIDSGS
                     DRYSPMEYWRALHNPDYRDVLQKTIVKHPSDWYFKKDDAIWQPFLNALKKDAPEWKKY
                     SEDFLDKMAWMQDVTTEKLGPSLWHMHPIMFLASLIQPNEDIRILRLRAFLRMIRIGE
                     GTIQEDGYRTMFTGVKFTDFSKHPNIRHEANGVVSTAAGAYQFLYGTWRNLQRRYSFP
                     DFSPSNQDLGCIALIAGRKALDVVMQGKISEAIHLCRIEWASLPGSPHAQPTANKKMI
                     MEKYELYLAEEKEGKTSLHATTEKIVKFIEGNYPEYL"
BASE COUNT          574 a          377 c          417 g          457 t
ORIGIN      
        1 acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac ggtcttgtga
       61 cgctaaaggg tttaccagaa aaaaccagca gtactctcac tcccggtcag cgctactggg
      121 ttgttgtgga taacaacaat gttaagccag cggcgggggc cgggcccggc tggtggcgac
      181 acttgctgcc tcccgcaaag gaagtcatgg tattcgataa aacagtaagc ctccgttccc
      241 cattcgccat taaggctggt gacccgattg ggcacatggg ctactatcag gcaccaaagg
      301 atggggggta tgaggcacga tatcaggtac atatcgaatg cacgagtatg gatgataatc
      361 tggagaaatt cctgaccaat cctgagcagg tgggaaaaaa taatccactc tggctgaagt
      421 acgccccggg acttgcgttg tacaagaaag atgtttctac aggaacattc acgaaggata
      481 cgggggtgac ggtcaggacg ggaattctgc cactgaaaca ggtgcaaaca gaagtcgata
      541 agtcgaccaa acaggaatac tggcaattgc ggccagaaaa cgcgtacgtg ccaaaaggtc
      601 aggtggaacc tcaacttttg tcacagtatg atctggcaaa gctggggttc agaactgaaa
      661 tcgcagagcc taccagcttt gattacctgg atggaaaaaa acagcccact ggattcttcc
      721 gcagtcttat caactcactc tatgaggctg cgatcgatga ttcgcgaacc agtcatgcgc
      781 tggtgaagca taattatcag cgcctgctgg ataaaattga tagtggaagc gatcgttact
      841 ctccaatgga atactggaga gcacttcata atcccgacta tcgtgacgtt cttcagaaaa
      901 caatcgttaa acatcccagc gactggtact tcaaaaaaga cgatgcaatc tggcaaccat
      961 ttcttaatgc gctgaaaaaa gatgctccgg aatggaagaa atacagcgaa gattttcttg
     1021 ataagatggc ctggatgcag gatgtgacca cggagaaact gggcccttcg ttgtggcata
     1081 tgcatccaat aatgttttta gcctccctaa ttcaacctaa tgaagatatt agaattcttc
     1141 gtttacgtgc atttctacga atgatccgca tcggtgaagg gacaattcag gaagacggat
     1201 acagaacgat gtttactggt gtaaaattta cagattttag taaacacccc aatatcaggc
     1261 atgaagctaa tggtgttgtt tcgactgctg ctggagctta tcaattttta tacggaacat
     1321 ggagaaattt gcaacgtaga tattcatttc cagatttcag tccgagcaat caagatctcg
     1381 gttgtatagc gctcattgca ggtcgaaaag cattagatgt agtaatgcag ggtaagatta
     1441 gtgaagccat acatttatgt cgtattgagt gggccagttt acccggttca ccgcatgctc
     1501 aaccaactgc aaataaaaaa atgattatgg aaaaatatga actctactta gctgaagaaa
     1561 aagaaggtaa aacaagttta catgccacca cagaaaaaat agtaaaattc attgaaggta
     1621 attacccaga gtacctatga aaaaattcat tttcctgtta ctaattccag tgttttgtaa
     1681 tgcaaacact cctactgttt tcaagtcttc ttgcagtggt attcaataca gtatcgaatc
     1741 attttgtagt gtaaataatc ttaatgatga ccaggataaa cttaccatac caatttgtag
     1801 caaacaaata tttacccttg gtaag
//
LOCUS       DAZKJR010000330         1814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKJR010000330 DAZKJR010000000
VERSION     DAZKJR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.332"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..536)
                     /locus_tag="ACHOWS_005280"
     CDS             complement(<1..536)
                     /locus_tag="ACHOWS_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0050690.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTSMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPP"
BASE COUNT          437 a          518 c          457 g          402 t
ORIGIN      
        1 gggggaacca tcagcagagt gtgaaaatgg ggatgtgcgc taccatcctt gccgcgcgtt
       61 acttccgtgg tacgaaccca gcccaacaca tggcgaaatt ccttacgctc tttcaaccgc
      121 ttaaaccccg cattcatcga ggtaagcgtc tcaccaagcg cgtcaatgga acagttgcgc
      181 actgtcagtg tgagaaacaa ccagcgcgcg ccgggatgtt ccgtcacaat ctttggcagg
      241 gactgataga aacgggcttg ccacatcatg gaacgcctcc actgacagat cggacagtgc
      301 cggacgcgac agaaccgtgc atcacgcaac cgcaggcgcg tttctccggt ctctgtgttt
      361 gttgaccacc caaaccgcaa aataccacta caagccccga cgcgttcgcc gtagtgctca
      421 aatccggcag cacgcagata aataccgccc acatcgtcgg aaaagcttcg gtgttcatcc
      481 cacggtttat ctgctggaga gtaatccgtc agattcttaa gttcaggatt tttcatgtct
      541 gccgtcccct tgcgagcgac agacgaaatt gcgtgttatc attcctgtat cacctgtacc
      601 aattgtcgtc aaatcagttc atgttctagg tggtcgcgcc cctggagtgg tggaagactc
      661 gcaggggcta atttataacc actcccgaca aaatactctc aaacctattt acacgcaaat
      721 ccgcgctcag gccagatatg gcaagggata gaggcgatcc aaaaaatcgc atttatccct
      781 taagtataaa tacaaggaaa cgcgcgcgaa gatagcagca aaatcgcctg ttttttgttc
      841 gattccccga gggcaaaaac aacaaccgcg aaagcgtttt cccgacaaaa cacacacagg
      901 cttttccacc gattttgtga acagcaaaaa ccgtcttgca ggtggttggt ctcactccgt
      961 gacaccaacc accatcccca gtgtttttcc cgtgccgtcg tcaatcacaa aatcggaacc
     1021 ccggaggggc ccccgcgatt ttctgtttga cgagttcgtg aacggtgagc gggacgcgga
     1081 attttttcgc gtgccgtgag gcgttggcgg ggtgtcgggg tgcagccctg accaggtggg
     1141 gaatgtctga gtgcgcgtgc gcggtccgac attcccacat cctgtcccga tttccgcggg
     1201 tttgtgttac cggaacgccg acgcaatatc ggcagttttt gcgccgtttc gccctacacg
     1261 gaagcgcgca gttctggcaa ccggacggca aatcattttg ccgttttcgt gccgcagccg
     1321 ttccgcgcga tcgacaaact ggcagtcggg ggaacctcac gaagtgcacg aagtgcgcac
     1381 ttatacaaac ttcacttcgt ttcgtttgtt gtgcggtctg cgacgctcaa aaaaccgaga
     1441 aacggcggga attttcggga caggatgtgt aatcgtataa ccgcgcacgc acgccgatac
     1501 gattacctcc tggtcagggc tgcaccccga caccctcgac aacctgcccg ggacacgtta
     1561 aaacgacatc tgcgcgtttc tggtgagcga aaagctgtca gccgtggatt gccgtagcag
     1621 agacccggaa acgcgcttaa aacgcttctg agcgcagaga aacaaaaaaa acgggcaaaa
     1681 ccttgcccgt ttctgaaaat cacgttagaa cgcttctgag cgcgtttctg aatattctcc
     1741 cagtgcatga atagttcgcg cctgaaacgc gctcaatcct cgccacttag ctgacgttta
     1801 tctttcgccc gatc
//
LOCUS       DAZKJR010000331         1793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKJR010000331 DAZKJR010000000
VERSION     DAZKJR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.333"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..266)
                     /locus_tag="ACHOWS_005281"
     CDS             complement(<1..266)
                     /locus_tag="ACHOWS_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFQ0050691.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIV"
     gene            complement(418..1158)
                     /locus_tag="ACHOWS_005282"
     CDS             complement(418..1158)
                     /locus_tag="ACHOWS_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFQ0050692.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1382..1567
                     /locus_tag="ACHOWS_005283"
                     /pseudo
     CDS             1382..1567
                     /locus_tag="ACHOWS_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          536 a          410 c          379 g          468 t
ORIGIN      
        1 acaataaaac tgttgttgat tttaccgtac gtcatacccg caggcgcata cagtgcacaa
       61 cagaacagaa ataacagagc tgtaatggat gtaagggtat ttctgtgtgc aaggagcaga
      121 agaaggaaca gcagcaacac attcccggtg gcattcttct cagtgtatcc gcatgcaatt
      181 gtggttatga cagaaataac aaaaaagaat aaaaacaata aaatccgtgg tgtgttgtcc
      241 ggacaaaaca gttttctgat attcatcgga gtatatcgac aacattattg tgaagagaat
      301 aaaaatcaga agtctcctgt gaaacagata acaaacaacc ctgcagtata attactgcag
      361 ggagtcttcg tttaattaca gaagatgggt aattattatc tttcaggagc agttttgtca
      421 gaaattataa gtcacaccaa gcatgatgtc atgactttta acatcaactt ctgatttata
      481 tttatcgccc cactcgtcct tgtaactcac actgctgtca cctgcatcaa gatagcgata
      541 gctgaggtcc agagcgatat ccggggttac gtcatagcga acacccgcgc caaggctcca
      601 tgcgaagttg tcagcagagc ctgaacgtga caacgattcg cgaccactgc ttccgtaccc
      661 ataatcccag gtactgatac cggttgtttt ctggtgaatt ctggcgtagc caatccctgc
      721 agatacccat ggtgtgaatg cactgtcatt ccggaagtca tagtacgcat tcagcatcag
      781 tgtgttgact gacacctcat tcttcaggtc atcacgccag taaccgcctg accagctgtc
      841 tttatctacg ttatacttcg aatcagcttt tccacgagcg taaaactcca gttccgtacg
      901 aaccggaata ctgaactgcg ggtaaaaatc ataaccggcc gcgataccgc cactgaatac
      961 cgtatcatgg ccatcaccgc ctttatattt tgatgtttcc tctccatcac ccgacaggaa
     1021 acgctggtct gcaagtgaca taacagaagc acctgcttta ccggtcacat aaaagcctgt
     1081 tttgctctca tcagccagag cctgggtcga aaacatgcct gccatggcaa gcgctgaaac
     1141 cgcaataacc ttattcattt caatcatctc cgctatcact ttccccgaac gataaatggg
     1201 gttgctcatt ctaaaaccat gcaaaagaca gggtaaaagg aatgttttcg atcgaatcga
     1261 tcgataaaat cgatcgtgcc cgataaagtt ttcttatcgt ccccataaaa atggtaaggg
     1321 ctggtttaag aggtaatcgc agcggcatgg aagtattaag accaaaacag cacgagataa
     1381 agcggctgaa cgtcagatga gccacatgga acgtaattca ttaagggcgg cttatatcca
     1441 taaaacagaa cacctggaat agcgtcggtt gatgctacag tggtgggggg attttctgga
     1501 tacaaactcg aaagaaaata ccagcccctt tgagtatgcg aagattaaca atccattaaa
     1561 atattaagca ctccgggcaa tctgcccgga gtgctttagc tggcataacg ggctactgat
     1621 ctcctctggt tacagataac gacttcagcc ctctgaaaga gaaaagcgga agctcaggaa
     1681 ggttgtgatt tgcttttaac ctttttaatt tagaagtatt attttgagat taatccactt
     1741 cccatcgtca tcctttttgg tgtgcgtgaa aagctatttt taaggttact gcc
//
LOCUS       DAZKJR010000332         1788 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKJR010000332 DAZKJR010000000
VERSION     DAZKJR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.334"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..486
                     /locus_tag="ACHOWS_005284"
     CDS             <1..486
                     /locus_tag="ACHOWS_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0050693.1"
                     /translation="NNAGVTRDNLINNMTEQDWDIVIDINLKGVFNITQAVAPIMMEQ
                     NHGAIITMSSVVGTDGNIGQSNYAATKGGVIAMSKGWAKEFSRKGAKVRVNCVAPGFV
                     ITPMTENLPEKVINHMIAKTPLGRMATPEDISNGVLFLASEKSSFITGQTLKIDGGLV
                     I"
     gene            496..1704
                     /locus_tag="ACHOWS_005285"
     CDS             496..1704
                     /locus_tag="ACHOWS_005285"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008913448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFQ0050694.1"
                     /translation="MEKVYITGAKRTPIGCFGGSLSTVSASDLGAIAISAAIKQSRIN
                     VECIDEVIVGNVLSAGQGMGPGRQAAVKAGIPWKTPALTLNMICGSGMKTIIDAAMKI
                     RSGDMDVVVTAGIENMSQAPYLIDHHARSGVRMGNQVMSDSLITDGLTDAFNHYHMGI
                     TAENIARQFNITRYEQDTFAFESQKRALKAIDNGEFEDEITPVQVHTRKGNIEVTTDE
                     YPRRDSSLEKLSVLRPAFEKNGTVTAGNSSGINDGAAALILASEKAIQTLELQPLAEI
                     RSFGQGGVDASVMGLGPVPAIDNALKKAKLDIKDIDLFEINEAFAAQAIGVLKSISEN
                     HSVSIDWLNQRTNVNGGAIALGHPLGASGSRIVVSLLYQMIKNNKKRGLASLCIGGGM
                     GTAVIIELCS"
BASE COUNT          587 a          263 c          415 g          523 t
ORIGIN      
        1 aataacgcag gagttacaag agataatctg atcaataata tgacagagca agactgggat
       61 attgtaattg atataaacct taaaggtgtt tttaatataa ctcaggcagt tgcacctatt
      121 atgatggaac aaaatcatgg ggcaatcata acaatgtctt ctgttgtagg aactgatggt
      181 aatataggtc agagtaatta tgccgcaact aaaggtggtg ttattgcaat gtctaaaggt
      241 tgggctaaag aattttcccg aaaaggtgct aaagtacgcg tgaattgtgt agctccagga
      301 ttcgttatta ccccgatgac agaaaatctg ccagagaaag tcattaacca tatgatagct
      361 aaaacccctt tagggcgaat ggcaacacct gaggatattt cgaatggtgt tcttttttta
      421 gctagtgaga aatcatcttt tattactggt caaacattaa aaattgatgg tggattagtt
      481 atttaaggag gaggaatgga aaaagtatac attacagggg caaaaagaac gcctatagga
      541 tgttttggcg gttcgttatc aacagtgtca gcttcagatc ttggagcaat tgcgatatct
      601 gcagcaatta aacagagcag aataaacgtt gaatgtattg atgaagttat tgtcgggaat
      661 gttttgtctg ctggacaagg aatggggcca ggtagacaag ctgctgttaa agccggtata
      721 ccatggaaaa ctccggctct gactttgaat atgatttgtg gtagtggtat gaaaactatt
      781 atcgatgctg caatgaaaat tcgtagtgga gatatggatg ttgttgttac ggcaggcata
      841 gagaatatga gtcaggcacc ttacttgatt gatcatcatg caagaagtgg tgtacgaatg
      901 ggtaatcagg taatgagtga ctctcttatt acagatggtc ttactgatgc ttttaatcac
      961 tatcatatgg ggattacagc agaaaatata gcccgacagt tcaatattac aagatatgaa
     1021 caggatacat ttgcgtttga aagtcagaag agagccttaa aggccattga caatggagaa
     1081 tttgaggatg aaattactcc ggtacaagta catacccgaa aaggaaatat tgaagttact
     1141 acagatgagt atcctcgtag agactcctca ttagagaaac tttctgtgtt acgtccagca
     1201 tttgaaaaaa atggtaccgt gactgcagga aattcatctg gaataaacga tggtgcagca
     1261 gctcttattc ttgcttcaga gaaagcaatt cagacattgg aattacaacc tttggctgaa
     1321 attcgctcat ttggacaagg aggcgttgat gcatcagtga tggggctggg gcctgttcca
     1381 gcaatagata atgcattaaa aaaagcaaaa ctggatatta aagatattga tctttttgaa
     1441 attaacgagg cgtttgcggc tcaggcaata ggggtcttaa aaagtatttc tgaaaatcac
     1501 tcagttagta ttgattggct aaatcaaaga acaaatgtta atggaggagc gatagctcta
     1561 ggccatcctt tgggagcctc tggctcaaga attgttgttt ctttattata tcaaatgata
     1621 aaaaataata aaaaaagagg tctcgcttcc ctttgtattg gtggaggaat ggggacagct
     1681 gttattatcg aattgtgcag ttaactttga aataagtatc tcttgcttgt caggagatac
     1741 ttaaataata atattaatat tgagagttat aatatgatag gcttgata
//
LOCUS       DAZKJR010000333         1786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZKJR010000333 DAZKJR010000000
VERSION     DAZKJR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.335"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..129
                     /locus_tag="ACHOWS_005286"
     CDS             <1..129
                     /locus_tag="ACHOWS_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFQ0050695.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHSRYVPG"
     gene            93..1181
                     /gene="tssG"
                     /locus_tag="ACHOWS_005287"
     CDS             93..1181
                     /gene="tssG"
                     /locus_tag="ACHOWS_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFQ0050696.1"
                     /translation="MERESQPVRTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPNEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            1217..1741
                     /gene="tssJ"
                     /locus_tag="ACHOWS_005288"
     CDS             1217..1741
                     /gene="tssJ"
                     /locus_tag="ACHOWS_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFQ0050697.1"
                     /translation="MVTVIRWWVPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDVAGYQMLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
BASE COUNT          376 a          503 c          531 g          376 t
ORIGIN      
        1 gagatgctgc accgtttctt tgcgctctat gctgatgttc accttttcaa ccagctcaca
       61 ctggtactgc aacccacagg gaaacgactg agatggaaag agaatcacag ccggtacgta
      121 ccgggctgac gcaggccctg gcccttaaca acgatatctg gcgggccagt ttttatcgtt
      181 tctgccagct gctggagcag gaaaatccgg acgggccaaa actgggcacg accagccatc
      241 cgggcgatga tccggtgcga ttcagaccct ggcccgggat gggattcccg gtcagcaccc
      301 tgaaagcggt ggagatcgac gaagaccgtc cgacattacc cccaacaatc cgcacgacat
      361 ttctcggaat gtatggggtg gactcacctc tgccgacgtc ctggctggat gatattacgc
      421 agcgccgtga aggacatgag gcactcacct catttctgga tattttcagc catcgcatca
      481 ccacgcagta ctaccggatc tggcgaaaat atgcgtaccc ggccactttt gaggaggggg
      541 ggcgcgatgc cacatcgcag tgtctgctgg ggctggtcgg gctcggtata cccggcaccg
      601 ctgaacaggt ggcaacgccg gtttcccgtt tcctggcctt actgggtgcc atgcgattac
      661 ccacccgcaa tgctgaaggc atccgggctc tggtgagtct gctggcaccg gatacccgcg
      721 cacttatcac cgaacccgat ccggttaagg tccatatcgg taaccgcagt ggactgggtg
      781 ccggaaaccg catccgtctt tcgcagcggg cgccgctggg taaaacgaca ggggaagcat
      841 gcagccggct cctgatgaca ctggaaacag cagatccgaa cgaagcggaa ggctggttgc
      901 ccggtggtgt cctccatacc gatttgctgg tcctgctgcg tgtttatctc ggctaccgca
      961 gtgacgtccg gctgcgactc actgtccccg tgcggttgtt gcctgagccc cgcctgggca
     1021 aaggcaggcc tgttcagctg ggccgtaccg gtttgctggg gctgaaagcg ggcaagctca
     1081 gcaatggccg ggagagtctg acggtcagtc tgggctgcta cgaagggttg aaatgtgctg
     1141 cgctaccgcc tgctgaagac ggacattatc gcttcgaata atcacctgtt ctctctctca
     1201 ataaaggagc gcctgaatgg taacagtaat ccgctggtgg gtgccgatgt ttattgcctg
     1261 tggcctgctc gcaggctgcg gtctgacgca gaccgtcacg gacggcacga tcagcatcac
     1321 gaaatccatc ttctacaaaa aaatcaaaac gctgcatctg gattttaccc cacgcacggc
     1381 cattaacact gacggggcac agacgccact ggcaaccatg gtgcgggttt accagctcag
     1441 ggatcgtaaa gccgttgatg tcgccggtta ccagatgctg ctgcgtaatg ccgatacggt
     1501 acttaaggac gacgtgctgg cctcaaaaga gctgctggtg atgcccggcg gcagcgtgac
     1561 gctcaatatg cccatggatg aagatgcgca gtttgtggct gtggtgggat tgtttaaccg
     1621 gccggatcag aaggacaatc gctggcgcct ggtactgacc cgtgatgacc tcaatccgga
     1681 taagccccgc accattgaac tgggtgatgg ctggctcagc cttgtgccgg taaaggagtg
     1741 acgtgatgga taaccggtca gcgttttttg acgggcagac ccgtta
//
LOCUS       DAZKJR010000334         1785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKJR010000334 DAZKJR010000000
VERSION     DAZKJR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.336"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..577
                     /locus_tag="ACHOWS_005289"
     CDS             44..577
                     /locus_tag="ACHOWS_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFQ0050698.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATQVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            868..999
                     /locus_tag="ACHOWS_005290"
     CDS             868..999
                     /locus_tag="ACHOWS_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050699.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            963..1117
                     /locus_tag="ACHOWS_005291"
                     /pseudo
     CDS             963..1117
                     /locus_tag="ACHOWS_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1182..1643
                     /locus_tag="ACHOWS_005292"
     CDS             1182..1643
                     /locus_tag="ACHOWS_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFQ0050700.1"
                     /translation="MNRILYVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREAPTTSGVDNATAPRLADTVERDYFTLRERLMLMQKQLEGAQDYIRTQC"
BASE COUNT          432 a          399 c          512 g          442 t
ORIGIN      
        1 attaaagaag accggcgtaa ggcggcgcgg ggagagtaag ctgatgagca ggaaattccg
       61 ctatggttta tcagcggtcg ttctggcgct gattgctgca ggtgcttctg cgcctgaaat
      121 cctcgaccag tttcttgacg aaaaagaagg taaccacacc acggcatacc gtgatggtgc
      181 gggtatctgg accatctgcc gtggtgccac ccaggtggat ggtaagcctg tcgtccccgg
      241 catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc attgaacgtg ataaggcgct
      301 ggcatgggtg gcgaaaaaca tcagagtgcc actgaccgaa cctcagaaag cggggatcgc
      361 gtcattctgt ccttacaaca ttggccccgg taagtgtttc ccgtcgacgt tttataaacg
      421 aattaatgca ggcgatcgca ggggagcgtg tgaagcgatt cgctggtgga ttaaggacgg
      481 tggcagagac tgccgtatcc gctcaaataa ttgctacggt caggtatccc gtcgcgacca
      541 ggagagtgcg ctggcatgtt ggggtatcga caggtagcag aatattttgc tgaaaaataa
      601 cgttggtcaa cgcggacgga taacacgaaa tcctgcgaac tggcaaaacc taagtgaata
      661 aaagtaaaaa ccccgttttt ggcagcaagc ggggttttgt gtttatggca gtaagctatg
      721 ggtggctgcc ttgattgatt ttagcaaact gattagggag ttgcgactca tgattagtca
      781 attaccaaac tggaagtttt tgctggtctg gagcatccct tttttatggg gggtatccca
      841 gttaattgtg gcaattaagg ggtagctatg tcagacaaac tcataacgct ggcgaagatc
      901 ctctgtgtaa tcgtcggcat ttcattttca ctaatgctgg ttgctatttt tcttttcctg
      961 ccctggatga tgttgtcttc gtcggggatg ctggggtaac agtgactgat gacatcagca
     1021 gagcgctggc tttgctatta agtgggtggc ggttggtatc gctgtgtctc cgatactgta
     1081 tgggctggca aaactggtca ttgcgctgaa atcgtgaact ttatttaaac agatgagtaa
     1141 tgaaattgct gtactggtcc gggcaattcg ctggtggtga tatgaaccgt attctgtatg
     1201 tggtgattat tgtactgctg gtggcctgtg gtgcgcttag tctggggctg aatcattacc
     1261 gcgataacgc catcacctac aaagcgcagc gcgataaaaa agccagagag ctggagctag
     1321 caaacgcaac cattactgac atgcagcagc gccagcgtga tgttgctgcg cttgatgcca
     1381 gatactcaag ggaattagcc gatgcgagag ctgaaaatga aactctgcgc gctgatgttg
     1441 ccgctggtcg taagcgcctg cggatcaacg ccacctgctc cggtaccgtg cgtgaagccc
     1501 ccaccacctc cggcgtggat aatgcaaccg ccccccgact ggcagacaca gttgaacggg
     1561 attatttcac tctcagagag cggttgatgc tgatgcagaa gcagctggaa ggggcacagg
     1621 actatatccg cactcagtgc tgaactaagt tttgctgatg cgccgtatcg tcgccgtatt
     1681 cccgcataaa cagagaccgc agccacctta tctgcgtgag tgtgcggggg taatcaaaaa
     1741 cgatgcacac cgggttttct catttttcac gagatgggag cgatt
//
LOCUS       DAZKJR010000335         1753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKJR010000335 DAZKJR010000000
VERSION     DAZKJR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.337"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            631..1533
                     /locus_tag="ACHOWS_005293"
     CDS             631..1533
                     /locus_tag="ACHOWS_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFQ0050701.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            1566..>1753
                     /locus_tag="ACHOWS_005294"
     CDS             1566..>1753
                     /locus_tag="ACHOWS_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050702.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADY"
BASE COUNT          513 a          320 c          437 g          483 t
ORIGIN      
        1 cttttattct tttcatttgt ttgccagcac ttcctgcgac tgtgcgcgct gtataagacc
       61 atttgaaatt tggtcgctta cgcgtgtatc tggcgtctga agctgcgcgg cgtcactttc
      121 gtcagcgtgt cacgatgaca aagtgacgca cgcgcgtctg cttaacgaac ccgcacgaac
      181 tgccgtttca cgtgatgttt tttaacattt gaagaagtga cccggcgtga gagtgttttt
      241 tcaacctctt ctgcaaattt acggacgatt ttgtgaataa cgtccataaa tggttttttg
      301 cagaatcaat tgttttttaa ggagttttat gtagtgagat gtcgaatcgg cgataccact
      361 tattagaggt ggtgtcgggg gcgacctgga gacaggtaca gcaaatgctg cagtcggtgt
      421 gcaacaccga tttgttaccc ctcaatacct aaacaatctg cgctaccctg gcgcacaggc
      481 agtcactacc cgaaggatcg caacgtttaa ccgtgtcgtc agggagaaat gcagatgagt
      541 ttttctgttg tgttcacggc acaacagaaa aactcatctg ccgaacgtac agctgtttaa
      601 ggtaaaaatt attatgtgca ggagaagttg atgtccagac gtgaaaaatt actgaaacag
      661 gatctggttg ctcacattcg aaaagacaaa ggcagcttca agaagctgag tgattacacc
      721 aggatcatgc accgctttgc tgaaagtctg gcaaaaatga atgtacagat cagttctgcc
      781 gcgcaggtaa aagtccggca tattgagcta tatatgagaa gccggagtaa tgtttctggg
      841 cgaacaaggg aaaatgaaat gtctgcaata cgggttatgt tacgtcaggc aggtaaatat
      901 aagatggctg atcccgaaca tcctcgtctc agcaataaag cgttaggcat ttccggctcc
      961 agtcgtaagg gaacaaaaaa attaatcacg gatgagcgtt ttcgggagat tcttcagcgt
     1021 gtggaagcaa aagataaagg ggtggctgct gttgttaaac tgtcacgtta cctcggtctg
     1081 agaaatgaag aagctatcca gtcggccaaa tcattacgaa cctggaaaaa agcgttacaa
     1141 cggggagatg aaaaaataag agtgattttt ggtacgaaag ggggacggga aagaatgaca
     1201 acggttgttg accgggataa cgttcttgat gctgttaatt atgcactcaa atatgccagt
     1261 gaacataatg gacgtcttat agacagagat aatcttaagt cggcaatgga ttattatctt
     1321 aatacattac gtcgttatgg agaactgaaa ggcggagatg agactgcaca cagttttcgg
     1381 tacagttacg ctgcaagtgc catgaaatat catattatga acggttacag ttataaggag
     1441 gcacttgcac tgacatcaat tgatcttggt catggtgatg gcagaggacg atatattgaa
     1501 caggtttatt gtcagttgtt aggctatgag taataactca tggcgatttc gtacgggtaa
     1561 atattatgga ttgtactatt ttgctgaaaa taatctctgt aaaagagatt acaaaaaatt
     1621 cgcctttgtg gtcacactat ctggcgcgat atgttgattc cggatatcag gccgagtttg
     1681 aatgtgaagg aacagcagat aaatgggata tctatgatga atgggatatc tatgaatcca
     1741 ttgcggatta ttt
//
LOCUS       DAZKJR010000336         1738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKJR010000336 DAZKJR010000000
VERSION     DAZKJR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.338"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..589
                     /locus_tag="ACHOWS_005295"
     CDS             <1..589
                     /locus_tag="ACHOWS_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFQ0050703.1"
                     /translation="VDKNIAEAVVSDEGATGGKALQLNKPESHIWFLEHDAAGQGAEL
                     LKKGGRVSVRFKVPGALVPNQFALGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATN
                     LNAMYHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPAS
                     GAADKLQLTGISKAATYTLLIDSVKVEVNNADAAA"
     gene            740..922
                     /locus_tag="ACHOWS_005296"
     CDS             740..922
                     /locus_tag="ACHOWS_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFQ0050704.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLIP"
     gene            960..1205
                     /locus_tag="ACHOWS_005297"
     CDS             960..1205
                     /locus_tag="ACHOWS_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFQ0050705.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            1282..1497
                     /locus_tag="ACHOWS_005298"
     CDS             1282..1497
                     /locus_tag="ACHOWS_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFQ0050706.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            1501..>1738
                     /locus_tag="ACHOWS_005299"
     CDS             1501..>1738
                     /locus_tag="ACHOWS_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFQ0050707.1"
                     /translation="MNKQYELVVKGINNYPDKITVTVALEIAGQPSLLSPYVAISLDR
                     TEGAKLEFYEAEAKKKAKQFFMDIAAALCEGDEQS"
BASE COUNT          452 a          376 c          528 g          382 t
ORIGIN      
        1 tgttgataaa aacatcgctg aggctgtggt cagtgatgag ggtgccaccg gtggtaaggc
       61 cctgcaactg aacaaaccgg aaagccacat ctggtttctg gagcatgatg ccgccgggca
      121 gggggcagag ttgctgaaga aaggcggacg tgtgagcgtg cggtttaagg tgccgggcgc
      181 actggtgccg aatcagtttg ccctgggtat ttactggcag ttgtcgtccc tgccggaggg
      241 agtgacgctg tcagaggaag gcaacgacat gctgatgtcc ttcttcctgc agacggatgc
      301 gacgaacctg aacgcgatgt accacaagaa gcagaatgcg aagctggata cgttcggggt
      361 ctttgataac ggatggcaca cactggcttt tgagtttgcc ggaaacaaca gcattcaggt
      421 gacgccggta ctggatgaga aacggggtac accgttcaca ctggtgaaat ctccggcatc
      481 aggggcggcg gacaaactgc aactgacagg catatcaaag gcggcgacat atacgctgct
      541 gattgacagt gtgaaggtgg aagtgaacaa cgcggatgcc gcggcatgat aaaaaaggcc
      601 gccagcaatg aaggaaaggg atgaactttg tccactacat gaatgctggc ggctggtaaa
      661 acgaccatga tgacaccgaa gtctgcggtg tttttctaca aaaataagta aatcatgtca
      721 actatcacgg tgagaaacca tgacatttct gaaccagtta atgctgtact tctgtacggc
      781 ggtctgtgtg ctgtatcttc tttcgggtgg gtacagggct gtgcgcgatt tctggcgcag
      841 gcagattgat aaaagggccg cagagaaaat cagcgccagt cagtcagccg gaagcaaacc
      901 cgaagagccg ctcattccgt agtcactttc ttaacaacac ctttcaacga gaaaatccta
      961 tgtcagaaat aaaatcgctg gtcactgctg aagcagtgaa ggaagtcctg cgctctgaag
     1021 aagtcagaag cgcactgaaa cagaaactcc gccagaacct tgagtcgcgt cttgatgcag
     1081 aagtggatgc cattctggat gaactgctgg gcgcaccggc agttcctgag ccggaaggca
     1141 ttgcgggtga cgggagtgct tcagatggcg gtgaacccac acccgacagc gacatgatga
     1201 tgtaagcatg cgcaagggac catcggtgtg tgtcggtggt ctttatattg ttgtgagctt
     1261 ccggattgcg ggagacgggg tatgtaccag atggaaaaaa tcacaacagg tgtgtcatac
     1321 accacgtcag cggtgggaac gggctactgg ttcctgcagt tgctggacag ggtttccccg
     1381 tctcagtggg cggcaatagg cgtgctgggg agtctgctgt ttgggctgct gacatacctg
     1441 actaacctgt atttcaagat taaagaagac cgtcgtaagg cggcgcgggg agagtagacg
     1501 atgaataaac aatacgaact ggttgttaaa gggataaata attacccgga taagattact
     1561 gttactgtgg cactggaaat tgccgggcag ccgtcgttgt tgtcgccata tgtggcgatt
     1621 agtcttgacc gtactgaagg tgccaagctg gaattttacg aagctgaggc gaaaaagaag
     1681 gcgaagcagt ttttcatgga tattgctgcc gctttatgtg aaggggatga acagtcac
//
LOCUS       DAZKJR010000337         1730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKJR010000337 DAZKJR010000000
VERSION     DAZKJR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.339"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            241..624
                     /locus_tag="ACHOWS_005300"
     CDS             241..624
                     /locus_tag="ACHOWS_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050708.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            717..1694
                     /locus_tag="ACHOWS_005301"
     CDS             717..1694
                     /locus_tag="ACHOWS_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFQ0050709.1"
                     /translation="MKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSADALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
BASE COUNT          382 a          406 c          522 g          420 t
ORIGIN      
        1 ttgacagctt actgctgcag tttttgtccg gtcaggggcg acagttatcc ccggatgact
       61 gggcagcact ggaagaggcc gaatatcagc tttactggct gcgggctctt gccggtgggc
      121 ggtaacagaa cgccgtagtt tctttatccg gaataacgtc atcacgtatt ccgtcattat
      181 atcacccggc agggacatat ccctgtgggg agagtcccgg ctttattcag gagaattgtt
      241 atgggactcg atatttactt tctggctcgt gataaccatc acagtggtgc cgttgtggga
      301 acacaggaac atcaggaggt gggggctttc cgtaaggtga aaccgcttct gacatggttt
      361 cgtgaacata ccggtgcttc agatgagcag catgctttcc ctgtaacccg cagttcactg
      421 gctgcattat tgcgcgacct cagtatgctg acaccgggga actgccacag gctgtttcct
      481 tatgtcacag gctcgtgttt acggggggaa gattatgccg gtgattactg gtatgacgtg
      541 gatgacgtaa aagcgtgggt cagcaatacg ctgaagacgt ttgattttga gcgtcgttca
      601 ctggtgatgc tggcgttgtg gtaattctgg ccagtctgat tttcgttatt aatttaactg
      661 tacggtgcac tctcaccgta cagggagcgt gcatcttttt cagggggcat gctcttatga
      721 aaacgaaaaa acatacttca cgtaccgcga ctaaaactcc ggaacgtgac ctgtatcagc
      781 ttgtgaccga cagggttgtt acggccattg aaaatggtgt gccaccctgg aaacgtccct
      841 ggcgctctgc gcgtaatggc gtggggcttc cggagatgat gccggcaaat gccctgaccg
      901 ggaagcctta tacaggggtg aacgtcctgt tgctgtggct ggccgcggat gaagccgggt
      961 atgtcagcaa tgactggctg acgtaccgtc aggcgcaaca actgggtggc cacgtccgta
     1021 aaggggaggt ggccacgctg gcggtgattt acaaagactg gaaaaaacag gctgtggatg
     1081 aacacggtaa ccggctgacg gacagtgagg gggagcctct catggaaatc gtaccggtac
     1141 tgaaatcgaa tcctctgttt aatgtggggc agtgtgacgg tttacctgaa catctgatgg
     1201 tgaaggcgga aggcagcgcg gatgcactgc cggcagatac cttcagtgag cgggtgctgc
     1261 aggtactgaa tggcagcggc gtcagcgtct cgtcacttcc acaggacagg gcattctacc
     1321 ggcctgatct tgaccgtatc gtgatgccac tgtcgtcgca gtttgaccag gtggctgatt
     1381 actggaccac cctgctgcat gagctggtac acagtaccgg tcatgaaaag cgcctgcggc
     1441 gggtggggat aagtaccttt accggtcgca acagcccgga atatgcgttt gaagagctga
     1501 ttgccgagat gggcagtgct tttctgtgtg cacatctcgg gattgccggt gacgtacagc
     1561 acgaaagtta tattgacgga tggcttaagg ttctgaagtc tgacaaaaaa gcgctgtttc
     1621 gcgcctgccg gcaggcacgt gaagcatcag agtatttact gacgcggcga acttcgcagg
     1681 agtgcgcagc ataattttta ctaccggtgg cggttataac ctgaccgcca
//
LOCUS       DAZKJR010000338         1722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKJR010000338 DAZKJR010000000
VERSION     DAZKJR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.340"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1437
                     /locus_tag="ACHOWS_005302"
     CDS             <1..1437
                     /locus_tag="ACHOWS_005302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050710.1"
                     /translation="IKVVVDDTGKYEINLNGWDIKDADGAEVKIVDSAGNVKSGNLYN
                     IARLYVDMNTNKAITLDSAYNIIGSQKEGTDPAKALIMSDDNDWVYIGGGISPHIGDQ
                     NPGSDNNIDMAGGDDILSSVGAVLDGANINMGDGDDKLYTQDGFASSGTRNIIMGDGN
                     DVISVDNSFGGKNTISLGEGNNLFIVGNYVNSIAENDITAGSGDDRIEIGTNLDGKNK
                     IDLGDGDNTIQVGGYITNSHTITGNSGDDIIYVATNIDGSGSFNLGEGNNNFIVGGYI
                     QGKNTIEMGSGDDTVSVSTRIADNVKIQLNAGDDSVYAGGLLNKAIVDLGDGDDVVTL
                     SGISDNGKRNNMEELVSTNAMLTGGEGNDTLKINGSFKLLNMKNISGFETIDLGESSE
                     NHLDVGIKSDMLDISSSSGVKIFTIMGGAGNTVDLGKANITSHNSVEQGNYADSWYKG
                     DTVDGYTTYTPVGDKSVELHIQQDILVI"
BASE COUNT          548 a          222 c          407 g          545 t
ORIGIN      
        1 ataaaagtag ttgttgatga cacaggaaag tatgaaataa atctaaatgg ctgggacatc
       61 aaggatgcag atggcgctga agtaaaaatt gttgattctg ctggtaacgt taaatcagga
      121 aacttatata atatcgcgcg tttgtatgtg gatatgaata caaacaaagc tataacgctg
      181 gattcggctt ataatataat tggctctcag aaagaaggaa ccgatcctgc taaagctctt
      241 atcatgtcag atgataatga ctgggtgtat attggtggag ggatatctcc ccatataggg
      301 gatcagaatc caggctctga caacaacatt gatatggctg gcggtgatga tattctgtca
      361 tctgttggtg cagttttgga tggagccaat attaatatgg gtgatggaga tgataaacta
      421 tatacgcagg atggtttcgc ttcttcaggt acccgaaata ttattatggg agacgggaat
      481 gacgttattt ctgttgataa ttcatttggt ggaaaaaata ccatttcttt aggagaggga
      541 aacaacttat ttattgttgg taactatgta aatagtattg ctgaaaatga tattacggct
      601 ggtagtggtg atgacagaat agaaattggt acaaatctgg atggtaaaaa caaaattgat
      661 cttggggatg gtgacaacac tattcaggtt ggaggataca taaccaactc acataccatt
      721 actgggaata gtggtgatga tattatctat gtcgctacaa atatagatgg ttctggctca
      781 tttaatttag gtgaaggtaa taacaacttt atagtcggag gatatatcca ggggaaaaac
      841 acaattgaga tgggttccgg tgatgatacg gtttctgttt ccactcgtat tgcagataat
      901 gttaaaatcc aacttaatgc cggggatgac agtgtgtatg caggtggatt gttaaacaag
      961 gcaattgttg acctcgggga tggggatgat gtcgtcacat tgagtggaat atcagataat
     1021 ggtaagcgta ataatatgga ggagttagta tccactaatg caatgcttac aggaggggaa
     1081 ggaaacgata ctctgaagat taatggttct ttcaaattgt taaatatgaa aaatatatct
     1141 gggtttgaaa ctattgatct tggggaaagt tcagagaatc atctggatgt cggtataaaa
     1201 tcggatatgc tggatatttc atcgtcatct ggggtgaaaa tttttactat catgggtggg
     1261 gcgggaaata cagttgattt aggtaaggca aatataacgt ctcacaattc agtagaacaa
     1321 ggaaattatg ctgattcatg gtataaagga gatactgtgg acggatacac aacgtatact
     1381 cctgtaggtg ataaatctgt tgaactccat atccaacagg atatattagt aatataattt
     1441 taatagcata aaagtggtcg catatgtgac cacttttatt tgttggcttt agttatttca
     1501 ggttttatta agaaagaagc taataatata catcatgttg ttatttgttg gtttaatgtt
     1561 aacatgtatg ataaaaatca tgtgggacgg gcatgtgccc ctccctttcc tttattatgt
     1621 ctgataatga aatgctaaaa ttatttttgt ttgtagactg tgttatatgc tttatggaaa
     1681 agtagtattt tgatttatgt gaagatgagc tgaactcgat tt
//
LOCUS       DAZKJR010000339         1715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKJR010000339 DAZKJR010000000
VERSION     DAZKJR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.341"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..556)
                     /locus_tag="ACHOWS_005303"
     CDS             complement(113..556)
                     /locus_tag="ACHOWS_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0050711.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAAYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            713..1642
                     /gene="metA"
                     /locus_tag="ACHOWS_005304"
     CDS             713..1642
                     /gene="metA"
                     /locus_tag="ACHOWS_005304"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFQ0050712.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          419 a          475 c          384 g          437 t
ORIGIN      
        1 aaaggccatc cgaaaggatg gcctttttgc attggcgcag aaaaaatgcc ggatgcgacg
       61 ctggcgcgtc tgctccagct tacgcaagca cgatagcacg atagggggca gcttattccc
      121 ctacatatgc cagattcagc aatggatacg gtttccccaa atcgtccacc tcagagcgtc
      181 ccgtaacctt aaaacccatc ttcttataga acccaacagc ctgcttattt tgctcattaa
      241 cattggttgt cagttccggt gccatcgaga gcgcatgttc caccagcatc cgacccacgc
      301 cgcagccgcg cacatcagga tcgataaaca gcgcatccat atgctgccca cttaacaaca
      361 taaacccaac cggccgatcc cgctcattaa ccgcgaccca caacggcgct tccggcagga
      421 aggaacgaac tagctcctcc agctcggccc gatacgctgc tgataaaaaa tcgtgagtgg
      481 catcgacaga acgacaccaa atcgcaacga gctcctcccc ttcctcatgc cgtgagcggc
      541 gaatactaat aaccattttc tctccttttc gtcattctta tattctaacg tagccttttc
      601 cttgaaactt tctcaccttc aatatgcagg ctcgacattg gcaaattttc tggttatctt
      661 cagctatctg gatgtctaaa cgtataagcg tatgtagtga ggtaatcagg ttatgccgat
      721 tcgtgtgccg gacgagctac ccgccgtcaa tttcttgcgt gaagaaaacg tctttgtgat
      781 gacaacttct cgtgcgtctg gtcaggaaat tcgtccgcta aaggtgctta tccttaacct
      841 gatgccgaag aagatcgaaa ctgaaaatca gtttctgcgc ctgctttcaa actcaccttt
      901 gcaggtcgat attcagctgt tgcgcatcga ttcccgtgaa tcgcgcaaca cgcccgcaga
      961 gcatctgaat aacttctact gtaactttga agatattcag gaacaaaact ttgatggtct
     1021 gatagttacc ggcgcgccgc tgggcctggt ggagtttaat gatgttgctt actggccgca
     1081 gatcaaacag gtgctggagt ggtcgaaaga tcacgtcacc tcgacgctgt ttgtctgttg
     1141 ggcggtccag gccgcgctca atatcctcta cggcattcct aagcaaaccc gcaccgacaa
     1201 actctctggc gtttacgagc atcatattct ccatcctcat gcgcttctga cgcgtggctt
     1261 tgatgattca ttcctggcac cacattcgcg ctatgctgac tttccggcag cgttgattcg
     1321 tgattatacc gatctggaaa ttctggcgga gacagaagaa ggggatgcct atctgtttgc
     1381 cagcaaagat aagcgaattg cctttgtgac gggccatccc gaatatgatg cgcaaacgct
     1441 ggcgcaggaa tttttccgcg atgtggaagc cggactagac ccggatgtac cgtataacta
     1501 tttcccgcat aatgatccgc aaaatacacc gcgagcgagc tggcgtagtc acggtaattt
     1561 gctgtttacc aactggctca actattacgt ctaccagatc acgccatacg atctacggca
     1621 catgaaccca acgctggatt aatcttctgt gatagtcgat cgttaagcga ttcagcacct
     1681 tacctcaggc accttcgggt gcctttttta tttcc
//
LOCUS       DAZKJR010000340         1699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKJR010000340 DAZKJR010000000
VERSION     DAZKJR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.342"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..186
                     /locus_tag="ACHOWS_005305"
     CDS             <1..186
                     /locus_tag="ACHOWS_005305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050713.1"
                     /translation="MNTELPDGRIALLRFHDPRVQKRLGEMLNDQQHRELTGLMQEWL
                     TIVDGKAWSFKQREFIC"
     gene            180..524
                     /locus_tag="ACHOWS_005306"
     CDS             180..524
                     /locus_tag="ACHOWS_005306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050714.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVRDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            524..1288
                     /locus_tag="ACHOWS_005307"
     CDS             524..1288
                     /locus_tag="ACHOWS_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFQ0050715.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQQIQ
                     EFKYCGVSFDGWKDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAVAT
                     VNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQP"
     gene            1285..>1699
                     /locus_tag="ACHOWS_005308"
     CDS             1285..>1699
                     /locus_tag="ACHOWS_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050716.1"
                     /translation="MIELFNTSINIHSLKERDITIDDCLNELYRSTVVVKNLTQHDHI
                     WYLTGYSRKDASKHVVFSDNEPNQNTIDTFERHYKKNRPLINESIWNGQPDGLACSIS
                     HFMKFIDNPKKLNLIIDIDQRVANTSDMIDSLLLLA"
BASE COUNT          526 a          297 c          448 g          428 t
ORIGIN      
        1 atgaatacag aactaccgga tggacggatt gcgttgctgc gtttccatga tccgcgcgtt
       61 cagaagcgcc tgggagaaat gctgaacgac caacaacatc gggagctgac ggggttaatg
      121 caggagtggc tgacaatagt ggacggaaaa gcctggtcat ttaaacaaag ggagttcata
      181 tgctgaagtt gtctgaagag cagtgtgttg gtatggacaa aagggaagat gatgattttg
      241 ttcgcgacac cgcgctaaga ctgaagaaga aatttccggg aattactgaa tcagatgaca
      301 cgttccatgt tcgcctgaga aaagcgctgg attatgccaa tacccttccg cttaaagaga
      361 aaaacgtgaa gcgggacttc ttgtttctgg aggccttctg gcctggtttt tatctgaaac
      421 ccgaagtgga taaatggttg agaacgccga atgcttattc aactgaccag cgggttgaag
      481 attataagca tgtgatgata aacagagaaa ggagggagct ctgatggtta tggtcctgac
      541 aggggctggt atggggagcg cagctaccgg aggtgttggt atggctggtg cctcaaccgg
      601 aacagcagta tttcccggag caggaagccg cgatggagac tgggaagaag ataatatatc
      661 ttatcctggc tcaggcagtg gttcgcgtca gggtaacgga gggttttatt atcaggggaa
      721 acagtatgat ccaattctga atgagccgat ggttgtcagc gcagagacgc atggtgattt
      781 ctgggaagat gatatggatc attcagaaac agtaaactat gaaggtattg atagcaaagc
      841 cgaagtacag gcgaagacgg atgagaaaac gtgtgagaac tgcccaccgg aagggaaggt
      901 aatgccgatg ataaggcgtt gctcgcgctg gagtatggta acaatcagct accagacaag
      961 aatatgtggc acgttttata atccggagac gcaacagata caggaattta agtactgcgg
     1021 cgtatcgttt gatggctgga aagataagct gtgtcagttc tgggaagcta aagcgcggta
     1081 tgaccaattt ttcagggctg atggttcaaa gaaaccctgg tggacaggta atctaagcgc
     1141 aattaatcaa gcatcccgac atcaggcggt tgcaacagtc aatcagccat tgaaagttgt
     1201 ctggatattt atgcaacctg taagttgtga gtatttcagg aaaatgttta aagattacaa
     1261 ggatattatt acgaggtggc aaccgtgatt gaattattta acacgtcaat aaatatacat
     1321 tctttgaaag aaagagacat aacgatagac gattgtttga atgaactata cagaagtacc
     1381 gtagttgtta aaaatctaac gcagcacgat cacatatggt acttaactgg ctactcaaga
     1441 aaggacgctt caaagcatgt cgtgtttagt gataatgaac cgaaccaaaa tacaatagat
     1501 acctttgagc ggcattataa aaagaatcgt cctttaatta atgaatctat atggaatgga
     1561 caacccgatg ggctggcttg ctcaatatca cattttatga aatttattga taaccctaaa
     1621 aaactaaatc ttatcataga tattgatcag agagttgcca atacttcgga tatgattgat
     1681 tccttgcttc tgttggctg
//
LOCUS       DAZKJR010000341         1698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKJR010000341 DAZKJR010000000
VERSION     DAZKJR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.343"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..408)
                     /locus_tag="ACHOWS_005309"
     CDS             complement(<1..408)
                     /locus_tag="ACHOWS_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFQ0050717.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDC"
     gene            complement(405..788)
                     /locus_tag="ACHOWS_005310"
     CDS             complement(405..788)
                     /locus_tag="ACHOWS_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFQ0050718.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWRCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(1160..>1698)
                     /locus_tag="ACHOWS_005311"
     CDS             complement(1160..>1698)
                     /locus_tag="ACHOWS_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050719.1"
                     /translation="EHMDGMFQRFSKLLPFRIDFAYRKDTPSFGHSCKHSMCMEIYRL
                     LSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNSYQISRQLGDIWRRITEGEG
                     YFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAKQDQKEHGIILGRSRLPEKS
                     NRGRPRLDSTLSEICSLV"
BASE COUNT          412 a          447 c          420 g          419 t
ORIGIN      
        1 gcagtcctgg cacaaatctg gctggtacct gtaaacagac ttcgtacatg ttgctctgca
       61 ataaatcccc gtaccacagg cctcacagaa tttcttccca tgaaaaaact gctcacacat
      121 cacacaatgc cactccagca cgaccggtaa tggcgataga aacatcgcca tatcctcaat
      181 gtaagggtgg gacttttccg gattcagcac cacgcaggtc gccttctgtt gcgcgctcag
      241 ggcatgtgaa tcatgctcaa accacgcccc ctgagcatct gtctgcaaaa tcaaccgacc
      301 acgacaggaa aggcagaaac actgccggat atttctgctg agcgcagagt cggcactgat
      361 aatgtgttca cccggcgtga tccccagccc cgtttttata ccgttcattc agccactccc
      421 tcctcactga agtgccctgt atggcagtgt gtgcagtacc gctccccata ataatcgtgg
      481 tgacattgtc tgcagcgcca gctggcttta cgcaccacgg gtaaggcatc cgggacgaat
      541 tgctgcagac gtttaatcaa ccgtatttct ctgcgttccg gcctgacata aggacattgc
      601 tgcgcgtgct ccgtcagccc ttcgtctgcg tgttcaaacc agggacgctc agtgtcgtat
      661 tgcgggtggt atctgagcgc actgccgcaa aggtggcaag tgtagcgatc gtaaggtgca
      721 gtctgtgcgg tacgggcacc cgtcagacgc ccgttgccat caaatgcaat aaaggatttt
      781 gcgtacatcg tcatattcct cagccgccag acgacacgca ggcaacagct gtcccggtac
      841 gggcagtgtg gtcagggtgt gaatggcggt tcagttaaaa tctggactgg agtttgggta
      901 aaacaggctg gttgctgtta ccgcactgtg gttgtcacac aatggcccac ctgcgtaaaa
      961 aactgtcagc aggcagggat ggacatcggg ggatgtgctg tatcggttgc cggaaggaat
     1021 ggtaaagaat gtgccatggt taaatctccg tatggttcat acggaatagc catgtcgtaa
     1081 aaaatgcgca gagtccctga agtggcctcc ggaaccgact gtaacaatta gcgccagccc
     1141 gtagttgtcg ttcaggggct tacaccagag aacatatctc cgacagggta ctgtccagtc
     1201 gcggtcggcc ccggttactt ttttcgggaa gcctgctacg ccccagaata ataccgtgtt
     1261 ctttctgatc ctgtttagcc aggtagctga gggcgtaacg taagtcgtct accgcagatt
     1321 tatcactata gtgaatcacg tgatcgatac gcaccgggta tttgtcttta gcccggcaca
     1381 gatgaaaata accctcccct tccgtgatcc gcctccagat atctcccagt tgcctggata
     1441 tctgataaga gttcttgtga cgttgtccat ccagatagcc gataaaatga atatgaagcc
     1501 ctttgtctgg tgtatattcc attacccagt aatatcctgc taacatcgtc tgggtttcgc
     1561 taagtaaacg gtatatttcc atacacatac tgtgcttaca ggaatgcccg aaactgggcg
     1621 tgtctttcct gtaggcaaaa tcaattctga aaggtaacag tttagaaaaa cgttgaaaca
     1681 taccgtccat gtgttcat
//
LOCUS       DAZKJR010000342         1692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKJR010000342 DAZKJR010000000
VERSION     DAZKJR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.344"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(254..421)
                     /locus_tag="ACHOWS_005312"
                     /pseudo
     CDS             complement(254..421)
                     /locus_tag="ACHOWS_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            571..1239
                     /locus_tag="ACHOWS_005313"
     CDS             571..1239
                     /locus_tag="ACHOWS_005313"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0050720.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGIVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1605..>1692
                     /locus_tag="ACHOWS_005314"
     CDS             1605..>1692
                     /locus_tag="ACHOWS_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFQ0050721.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGIS"
BASE COUNT          537 a          366 c          283 g          506 t
ORIGIN      
        1 ataaaaagag tatcattttt acttgcattt cttatcaagt cacattcaac aacagtaaaa
       61 aaacattatt agaaccattc aattaacaaa aaaaccaaca tccagcttgc ttaatttttc
      121 tttattaaac gatattgaaa atcaattgat aaaatacatc taaacaacct tttggggcgc
      181 aaaagcataa catcaaacaa ataacacacc gaaaaaactc acaattaata acctatgata
      241 tacatactgt ttattatggt tgaataagcc actcgatatc tggtgctacg gaagtgtcca
      301 cacggtttag cagcacccga tacttttttc aggcttccag caacgatctt tcttcctccg
      361 ttgcgatttc cagatctaca gcatcctgca gtggcgcaat atactcactg aattcctgga
      421 tgtagaactg tgtggtgacg gtcttccagc tattcggctc ctgctgtatt gaagcatacc
      481 aggctatttc aatatcgcta tgctgcggca gcatttaacc ccttgtaatt cattgccata
      541 attgatttaa ttcacaaata aaactataac atggtgaaat taatgaaaaa aaacacagat
      601 gatggggcta aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga
      661 gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg
      721 gaacatttag gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac
      781 gtacctgaga gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca
      841 tctacaaggg ctggggaatc aaaaattaaa cataaaatta accatgatgt ttttgaacct
      901 tatcccgcgg cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc
      961 ctgcctggaa atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact
     1021 gacgatggaa ctctatacgg agccacaatt cttggcgatg gaattgtgca caatagcttc
     1081 ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc
     1141 gaagaaggct taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa
     1201 ggtactgtag taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct
     1261 tgcataaggc gtgtctgcgg tttttcaact attcagatac atcactccca tcacattcat
     1321 tcctccgcat cataggcata taggctatat caccttgata tttttcttct tcagataaaa
     1381 actgttatct atgtatactt ttaaacccaa tccgtgtaga gtctctgcat aagatagttt
     1441 gcagttgaca cttcagcttg cgccataaac cgcctgattt ttgccgccac ctgttagcat
     1501 tcctgtatac ctgaaacgac aatgtttatc tacgaacttt aagaacaccc aggataaaaa
     1561 ttgtcaacta tatcatatat aacacattac taattcgagg ctatatgaac agcatactga
     1621 taatcacttc gctccttatc atattcagca tttttagtca tgctctaata aaattaggga
     1681 ttggcatatc ca
//
LOCUS       DAZKJR010000343         1654 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKJR010000343 DAZKJR010000000
VERSION     DAZKJR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.345"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..743
                     /locus_tag="ACHOWS_005315"
     CDS             <1..743
                     /locus_tag="ACHOWS_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFQ0050722.1"
                     /translation="FGGKGYFSYPKPTSLIKTLFGSVKTEDKDIYLDFFAGSGTTAHG
                     ILELNIEDGRKRNFICVQLDEETDKKKQAYKEGYKSIFNITKDRIIKAGKKLKKDNPN
                     YNGDLGFKIFETVHDFRAKDESELTLSNLSFFDDAVLTPEQYDTLLTTWCVYDGSLLT
                     TPIEDIDLNGYKAHLCDGRLYLIAPNFTSEALKALLQKLDADKDFAPNKVVFYGSNFE
                     SAKQMELNEALKSYANKKSIDLDLVVRN"
     gene            746..>1654
                     /locus_tag="ACHOWS_005316"
     CDS             746..>1654
                     /locus_tag="ACHOWS_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0050723.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDAIKEHFRDDYERELKTYVVESQKSSGKNTK
                     SYMPQAIHDFVEASNFNKKYIHILVINSGMINSKSLTDTYDVGLLDNQFDTPFSALSA
                     IKPFIIIDEPHKFPTGKKTWEHIQKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNED
                     LVKGIDAYIEDIVGDGNANLKLVKSDG"
BASE COUNT          580 a          283 c          304 g          487 t
ORIGIN      
        1 tttttggtgg gaaaggatat ttctcctacc cgaagccaac aagtttaata aaaacactat
       61 ttggttcagt aaaaaccgaa gataaagaca tttatcttga cttttttgct ggctctggaa
      121 ctacagctca tggtattctg gagttaaata ttgaagacgg caggaaaaga aacttcatct
      181 gtgttcaatt agatgaagaa acagataaaa agaaacaagc ctataaagaa gggtataaat
      241 ccatcttcaa tataacaaaa gacagaatta ttaaggcagg aaaaaagctg aaaaaagata
      301 atcctaatta taatggagat ttgggattta aaatatttga aacagttcat gattttcgag
      361 cgaaagatga gtctgaactt acgctttcta atcttagttt ctttgacgat gctgtattga
      421 caccagagca gtatgacact ctattgacga catggtgcgt gtatgatggc agcttgctaa
      481 caacaccaat tgaagatatt gaccttaatg gctacaaagc acacttgtgc gatggtcggc
      541 tatatctaat tgcgcctaat ttcacaagtg aggctttgaa agctttactt caaaagctgg
      601 atgcagacaa agactttgcc cctaacaaag ttgtatttta tggcagcaac ttcgaaagtg
      661 cgaaacagat ggaactcaat gaagcactaa aaagttatgc taataaaaaa tctattgatt
      721 tagatttggt ggtaaggaac taatcatgtc taaagggttc acattcgaaa aaaatttacc
      781 tcatcaaaag gctggcgttg attcggtaat gaatgtcttt gtatccgcca tacctcatca
      841 gacggatcat gttgcaattc gcctgttagc taatccagaa ctgaatctat ctgaacaaca
      901 atactacaac aacataaaaa atgttcagga gttcaatggt attgagcact caaaagataa
      961 ttacgatgct aaaagcaacg taattgatgt ttctatggag acaggtacag gtaaaactta
     1021 tacttacacc aaaacaattt ttgacctgaa caaatcattt ggtattaaca aatttatcat
     1081 cattgttcct accctatcga tcaaagccgg aaccgttaac tttttaaaaa gcgatgctat
     1141 aaaagaacac tttcgagatg attacgagcg tgaactaaag acttacgttg ttgaaagtca
     1201 gaaaagttcg ggtaaaaata caaaatcgta tatgcctcaa gctatccatg attttgttga
     1261 agcaagtaat ttcaataaga aatatataca cattcttgtg atcaactcag ggatgattaa
     1321 ctctaagtct ttaactgaca cttatgatgt tggcttgctt gataatcagt ttgatacccc
     1381 attttctgca cttagtgcga taaaaccatt cattatcatt gatgagcctc ataaatttcc
     1441 tactggtaaa aaaacatggg aacatattca aaaatttaat gcccaatata tcatccgcta
     1501 tggcgcaacc tttagtgaag gctataagaa cttagtttat cgtcttacag ccgtagatgc
     1561 cttcaatgaa gatcttgtta aaggtattga tgcatacatt gaggatattg ttggtgatgg
     1621 taatgccaac ctcaagttgg tcaaatctga cgga
//
LOCUS       DAZKJR010000344         1641 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKJR010000344 DAZKJR010000000
VERSION     DAZKJR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.346"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..715
                     /locus_tag="ACHOWS_005317"
     CDS             <1..715
                     /locus_tag="ACHOWS_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0050724.1"
                     /translation="HARRCAPAESCGFVISTPEGERYQPCVNISAEPEAYFRITPEDW
                     LQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHRFRCVPHLTGR
                     RFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAENGFYRVSPSCA
                     QAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRRTHSVWRHRHW
                     SASAFTGIYNDLVVASACM"
     gene            712..1293
                     /locus_tag="ACHOWS_005318"
     CDS             712..1293
                     /locus_tag="ACHOWS_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFQ0050725.1"
                     /translation="MNTAAEAIRALSLQVPGFRGQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTAGATMALWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            complement(1315..>1641)
                     /locus_tag="ACHOWS_005319"
     CDS             complement(1315..>1641)
                     /locus_tag="ACHOWS_005319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050726.1"
                     /translation="HPGGCLCHDGIKFLKLFTFPAIACSRNNDAALQFLWSIKQNSLK
                     LVFIVISRRTVNAAGFFYPRQKNLNFFTFSVRLRGRYHSGALHCPVREKQFILVNLHR
                     YFPQLR"
BASE COUNT          369 a          399 c          517 g          356 t
ORIGIN      
        1 gcatgcccgg cggtgtgcgc cagcggagtc gtgcggcttc gtgataagca ccccggaggg
       61 cgaacggtac cagccctgcg tgaatatctc cgcagagccg gaggcgtatt ttcgtattac
      121 accggaagac tggctgcagg cacagatgca gggggagatt gtggcgctgg tccacagtca
      181 tcccggtggt ctgccctggc tgagcgaggc ggaccggcgg ctgcagataa agagtgccct
      241 gccctggtgg ctggtctgcc ggggtgaaat tcaccggttc cgctgtgtgc cgcacctgac
      301 cggacggcgc tttgaacacg gtgtgacgga ctgttacacc ctgttccggg atgcatacca
      361 tctggcgggg ataacgctgc cggattttgt gcgtgaggat gactggtggc gcaacggtca
      421 gaacctgtac ctggacaacc tggcggaaaa cggcttttac cgggtgtctc cgtcctgtgc
      481 acaggcgggc gatattctgc tgtgctgctt tggcgcatcg gtgccgaatc atgccgccat
      541 ttactgtggc aacggtgaac tgcttcacca tatacctgaa caactgagta aacgggagag
      601 gtattcagag aaatggcaac gacgaacgca ttctgtctgg cgtcaccgcc actggtccgc
      661 atctgccttc acggggattt acaacgattt ggtcgtcgca tcagcctgta tgtgaacacg
      721 gcagcggagg ccatccgtgc cctgtcgctg caggtgccgg gattccgcgg tcagatgaac
      781 gaaggctggt atcagatacg tattgccggt gaggataccg cgccggaggc ggtgtatgcc
      841 cgtcttcatg agccactgag cgggagggcc gtgattcata ttgtaccgcg gctggccgga
      901 gccgggggaa atggtgtttt tcaggtggtg ctgggggcag cagccatcgt gggctctttc
      961 ttcacagccg gtgcaacgat ggcgttgtgg ggcgcagccc tgagtgccgg agggctgact
     1021 gccaccacga tgctgttctc actgggtgcc agcatgatac tgggtggtgt ggctcagatg
     1081 ctggccccga aggcaaagac gccggagtac aaaagtacgg ataacggtaa acagaacacg
     1141 tatttttcgt cactggacaa catgattgcc cagggtaacc cgatgccggt gccttacggt
     1201 gaaatgctgg ttggttcacg acggatatcc caggacatca gcacccgtga tgagggcgga
     1261 gacgggaaag tggtggttat cggacgggga tgaaaataaa aaaatcccgc agagttagcg
     1321 gagctgcggg aaataacgat gaagattaac aagaatgaat tgtttttctc ggacagggca
     1381 atgtaacgcc ccggaatgat atctgccacg gagacgtaca gaaaatgtga aaaaattcag
     1441 atttttttgc cgggggtaaa aaaatcccgc agcgttcaca gttctgcggg aaataacaat
     1501 aaagactaac tttaaggagt tttgttttat tgaccataaa aactgtaacg ctgcgtcatt
     1561 attgcggcta caggcaattg ccggaaatgt gaagagtttc agaaatttta ttccgtcatg
     1621 acacaggcac cctccggggt g
//
LOCUS       DAZKJR010000345         1598 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKJR010000345 DAZKJR010000000
VERSION     DAZKJR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.347"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1203
                     /locus_tag="ACHOWS_005320"
     CDS             <1..1203
                     /locus_tag="ACHOWS_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFQ0050727.1"
                     /translation="GMGWQVITGNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLD
                     EMDGFTGCVALWDTGKNGAVTAFRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHAC
                     GHDGHISIGLGLAQWVSENNHKLCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLS
                     SHIGMGVPTGNVVASPTDLLCTTKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLL
                     AIPRHGEGMSRINIGTLNAGEGRNIIPSFARMQLEVRGETETINQYMREHALKIAQGI
                     SSIFGLRFEYKVVGEATDLHNDPLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRR
                     VQKIGGKAAYIILGSDLKAGHHEVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGN
                     GKLESELY"
BASE COUNT          493 a          279 c          368 g          458 t
ORIGIN      
        1 ggtatgggat ggcaggttat tacggggaat aaatacctgt caacagagta tattatgggc
       61 cgtaatgcaa ctgaagttgc agatagtata aaacgtgcag ttgattcagg cgttccccat
      121 gaattgctgg atgaaatgga tggctttaca gggtgtgttg ctttatggga tacaggaaaa
      181 aatggagctg tcactgcgtt tcgttttgac attgattgtg tacaggtaag tgaatcaaaa
      241 tcgaaagatc atattcctga aaaaatggga tttgcatcca tacactctgg cttaatgcat
      301 gcatgtggac atgatggtca tatatcgatt ggtttaggat tggcacaatg ggtatctgaa
      361 aataaccata agttatgcgg gaaaattaag ctaatttttc aggctgcaga agaaggcgtt
      421 cgcggtgcaa aacctattgc agaaggtggt attcttgatg actgcgacta cttcctttct
      481 tcacatattg gtatgggagt tcctacagga aatgttgtcg cttcacctac agatttgttg
      541 tgtacgacaa aaatggattt ctttttctat ggtactccgg cacatgctgg tgtaaatcct
      601 cacctgggaa ataatgctct ggcagctgct tgtcactgtg caacgcaatt acttgccatt
      661 ccacgccatg gtgaaggtat gtcccggata aatattggca cgctaaacgc aggtgaagga
      721 aggaacataa tcccgtcttt tgcaaggatg caacttgaag ttcgtggaga aacggaaaca
      781 ataaatcaat acatgcgtga acatgctctg aaaatagcac aaggtatttc ttccatattt
      841 ggtttacgtt ttgagtacaa agtggttggt gaagcaacgg atttacataa tgacccttta
      901 cttgtcaatc tgatacacta tatagcacaa gaagatatga acatgtcttt gacagagaaa
      961 tcatttgggg gcagtgaaga tgcaacgctc cttattcgcc gtgtacagaa gataggagga
     1021 aaagcagcat atataatact tggcagtgat ttaaaagctg gtcatcacga agttgatttt
     1081 gattttgatg agaatgtgct ggatacaggc gttaaactgt tttcgttatg tctggagaga
     1141 ttgaactctc ccggactaaa taaaattgta gggaacggaa aactggaatc tgaattgtat
     1201 tgattgctga caggtatcct gtcaatcact cctgatttct gcctttaaat acagtctcca
     1261 ttaacgaata aagagaaaat ggagactttt atattcccaa aagagagaat aggattgtag
     1321 cgaattttaa ccaaatagac aaacacggct cagcatatac tggtgatatg ggaagaggat
     1381 gattgcgaga ttaccctatc ctaatttaca gaataagaga ttatatacag ataaaaaagc
     1441 ctcatactca tcacgtactg tttttgtatg acgcaacaat atcagcgaac gagccatttt
     1501 ccgccagcca caaagcctga cgcatctacc tgaattgacg gatgagcaaa ctggctggac
     1561 tctgtagtcc ggaatttacg ggaccttgtt attataat
//
LOCUS       DAZKJR010000346         1586 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKJR010000346 DAZKJR010000000
VERSION     DAZKJR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.348"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            416..>1586
                     /locus_tag="ACHOWS_005321"
     CDS             416..>1586
                     /locus_tag="ACHOWS_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFQ0050728.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVR"
BASE COUNT          370 a          300 c          510 g          406 t
ORIGIN      
        1 ggtcttaagt acaggagtgt gaagcacctg gttggacgtg cgtagccgga atgcatattc
       61 gtgagtcggg taatgagagt ggaacggtgg cagtgagaat ggaacagaaa aaaatcagat
      121 gcggaaagcg gaaatacaca gcgggtgcga tgtgcttcat gctgtttgta tggctggggg
      181 gtgtggtgac attctgtgtg ctggccgctc tgataatgag gtgaaaacgg aagagagttt
      241 acttccttaa ttcgaacttt ctgataactg agaacttgtt aaatgcctgt gggtttatgt
      301 atttaaatca attggttaat ggtgatttta tctttatcag tcaaagttcg ttttagtatg
      361 atgattgtgc ccgctgttta ttgttgtccg ggagaaaata cagggaatcc aggggatgaa
      421 caaaatatac tcactcagag ttaatgacag aaaagaactg gttgctgtac cggaaacggt
      481 ggggggcagg aaaaagtcgt ccggcattcc cggtgcgggg atgctttccc gtcttcttct
      541 ggcctcagga gccgttgccg gcgttctgtt ttcttctcct gcgctggcat ccgttgtcgg
      601 gaatactctg ccatggcaga cctacaggga ttttgctgaa aacaaagggg cgtttcatgc
      661 cggggccaca aatattcctc tttatggtaa gaatggggct gtcagtgggc ggctggacaa
      721 ggctccgatg atggatttca gtgttgtgga ccagatactg ggggtggcaa cactgatttc
      781 tccgcaatat gtggcggggg ttaagcataa cggcagctac aatacggtgc gttttggtta
      841 tgcggacgat acgacatatc gccttgttga ccgtaatgag cactggcgtg attttcatac
      901 tccccggctg aacaaactgg tgacagaagt tgccccggtg agcgtcacgg atgccggaac
      961 cggcaagggg gtataccaga accgcagtcg ttatccggtg ttttatcgta tggggagtgg
     1021 aacgcagtac accggagctg cttccggtgc gctgaccaga atagcggatg cttacgtctg
     1081 gaggactggc gggacggtgg gaagtccggt catttctgac tggagtctgg tgtcaaaccc
     1141 gggaaatctt taccagggtg ttaatggtcc gctggcgtct tacgggacat caggagacag
     1201 tggctcaccg ctttttgcct gggatgcggt gaagaagcag tgggtatttg ttgctgtact
     1261 tcatggttat gccggtgaga acgggaagac gaactggttt acggtgattc ctgcagggga
     1321 tgtgaataac acgataaagc aggacagttc aggcaccgtc gtgccggcag ttgccggtgg
     1381 tgacattgtg tggaattaca gtaagggaag cggagaggga accctttctc aggatgggaa
     1441 ggtatggaag atgaacggct tcaggggagg gagtctgaat gacgggaaag atatcacctt
     1501 tgggggtaag ggcacggttg ttctgaagga tgatgttgtc cagggggcgg gttcgctcac
     1561 gtttaatggt gattatacgg tcagac
//
LOCUS       DAZKJR010000347         1574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKJR010000347 DAZKJR010000000
VERSION     DAZKJR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.349"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..738
                     /gene="nanR"
                     /locus_tag="ACHOWS_005322"
     CDS             4..738
                     /gene="nanR"
                     /locus_tag="ACHOWS_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFQ0050729.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(774..>1574)
                     /locus_tag="ACHOWS_005323"
     CDS             complement(774..>1574)
                     /locus_tag="ACHOWS_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0050730.1"
                     /translation="PEHNGEFELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYRA
                     VMLDKEGTALAPEFLSQGYTLFVLSYRLPSPAEEENINKTFIDAISAMLYIRKNADKW
                     HLQRHRIGVLGFSAGGHLASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKD
                     DVTHPDSRKQLLGDNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYR
                     GLIEKGVSAEMHLYEKGGHGFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          541 a          330 c          282 g          421 t
ORIGIN      
        1 tatatgtccg acaacaaaaa aaccgtgtta cctgggctaa caacgaccag aaagcccatt
       61 gttacaaaaa aactttcaga aatcgttgcc gaagagcttg aaagaatgat ccgccaggag
      121 gagataaggg aaggtgaata cctgccttct gaacgtgaac ttatggcatt ctttggcgtt
      181 ggaagaccat ctgtcagaga ggctttatca tctctcagac ataaagggct aatccggtat
      241 aacaagggag aaaaagccag tgtctgccgt ccatcaccag aaaccatcat taatgggtta
      301 tcaggcattg ttaaagattt tttatccaca gctgatggca tcaaggattt tgaaagacta
      361 cgcatctttt ttgaaaccaa ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat
      421 attcacaggc ttgagaaggt gctcagtatc catcataact cactgagaac ccccgaagtg
      481 tatgctcaat atgatattga atttcatagt atacttgctg atatacctga caataaagta
      541 ttaaaaacaa tacactcagc attttctgaa tggctgataa tggccaaacc tctggaaaag
      601 aataaaatga catatgaaaa taacaaaaaa acccatcagc agcatattga aatacttacg
      661 gccataaaag agcatgattg tgataaagct gaacagctaa tgtataatca cctttataaa
      721 cgaattgaag catcatagtt actcgacgct tcaccaggta gtcaccataa atattattta
      781 aatccatgag aatacatcca gtccataaac agttttatcc atcctccaac aggaagatct
      841 gtggcccact gaacactaaa gccatgccct cctttctcat agagatgcat ttcagcagaa
      901 acgcctttct ctattaggcc acggtacatg agcagactgt tttcaattga cacaagatca
      961 tcatcacaac aatggagtaa aaacacagga ggaaaacatg atgtgacatt aagatccgca
     1021 ctatattgta ttatattttc tgaggtacta ttgtccccca gtaattgctt cctggagtct
     1081 ggatgtgtca catcatcttt cattgatatt acaggataaa ttaacccgac aaaatcggga
     1141 atagcgaaaa tctcttgttc tttactgtca gaagaacgtt tgaaatcaca aatctgattc
     1201 acaaacgttg aggcgagatg ccccccagca gaaaatccta atacaccaat acgatgacgc
     1261 tgtagatgcc atttatcagc attcttacga atataaagca ttgcagatat tgcatcaata
     1321 aaagttttgt ttatattttc ttcctcagct ggcgatggta atctgtaact aagaacaaat
     1381 aatgtatagc cctgagataa aaactctgga gcaagagctg taccttcctt atctaacata
     1441 acagcccggt agcccccccc gggaataatc aatattcctg ttccatttgg tttatctggt
     1501 aaaaaaataa ttaactgagg agcataaata tctgtcaata cacgatcaag ttcaaattcc
     1561 ccgttatgct cagg
//
LOCUS       DAZKJR010000348         1560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKJR010000348 DAZKJR010000000
VERSION     DAZKJR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.350"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..474
                     /locus_tag="ACHOWS_005324"
     CDS             <1..474
                     /locus_tag="ACHOWS_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFQ0050731.1"
                     /translation="APAPDILDQFLDEKEGNHTTAYRDGAGIWTICRGAIMVDGKPVI
                     PGMKLSKEKCDWVNAIERDKALAWVEKNIRVPLTEPQKAGIASFCPYNIGPGKCFPST
                     FYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            695..808
                     /locus_tag="ACHOWS_005325"
     CDS             695..808
                     /locus_tag="ACHOWS_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050732.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIIAIAVLIWAIRWW"
     gene            810..1277
                     /locus_tag="ACHOWS_005326"
     CDS             810..1277
                     /locus_tag="ACHOWS_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFQ0050733.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTVTRDYFTLRERLMTMQKQLEGAQEYIRTQCIK"
     gene            1301..1525
                     /locus_tag="ACHOWS_005327"
     CDS             1301..1525
                     /locus_tag="ACHOWS_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050734.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQPGRVLVEGTFSGKCTQSYSRVFR
                     VDASNEELTVECAVNAAKCRMVSAALQPVC"
BASE COUNT          385 a          350 c          453 g          372 t
ORIGIN      
        1 gcgcctgcgc ctgatattct cgaccagttt ctggatgaaa aagaaggcaa ccacaccaca
       61 gcataccgtg atggtgcggg tatctggacc atctgccgag gtgccatcat ggtggatggt
      121 aagcctgtga ttcctggcat gaagctgtcg aaggaaaaat gcgactgggt taacgctatc
      181 gaacgggata aggcgctggc gtgggtggag aaaaacatca gagtgccact gaccgaaccc
      241 cagaaagcgg ggatcgcgtc attctgtccg tacaacattg gtcccggtaa gtgtttcccg
      301 tcgacgtttt acagacgaat taatgctggt gatcgcaggg gagcatgcga ggcgattcgc
      361 tggtggatta aggacggtgg cagagactgc cgtattcgtt caaacaactg ttacggtcag
      421 gtatcccgtc gtgaccagga gagcgcgctg gcgtgctggg gtatcgacag ataagcagaa
      481 tattttgctg aaaaatgacg ttgaccaacg cggacggata acacgaaatc ctgcgaactg
      541 gcaaaaccta agtgaataaa agtaaaaacc ccgtttgttg gcagcaagcg gggttttgtg
      601 tttctgacct tggataaggc aagggagaaa ttatggtgag ggaggtgctt tccctgtgag
      661 gaagtataaa agattctttc tgaggttgtc cattatgaaa ggcattgaag tggagacgcc
      721 agccagtctg gatttaacaa gagcggcagc ttttgccatt cgtattatag ccattgctgt
      781 tctgatctgg gcaatccgtt ggtggtgaaa tgaaccgcgt tctgtgtgtg gtcatcattg
      841 ctctgctggt ggcctgtggt gcgcttagtc tggggctgaa tcattaccgc gataacgcca
      901 tcacttacaa agcgcagcgc gataaaaaag ccagagagct ggagctggca aacgcaacca
      961 ttactgatat gcaggtgcgt cagcgtgatg ttgctgcgct cgatgcaaaa tactcgaggg
     1021 aattagccga tgcgagagct gaaaatgaaa ctcttcgcgc tgacgttgcc gctggtcgta
     1081 agcgcctgcg tatcaacgcc acctgtccag gtcccgtgcg tgaagccacc ggcaccgccc
     1141 gcgtggataa tgcaaccggc ccccaactgg cagacaccgt tacacgggat tatttcaccc
     1201 tcagagagcg gctgatgacg atgcagaagc aactggaagg ggcgcaggaa tatatccgta
     1261 ctcagtgcat taagtagtct ttttattatc cggaggatat atgaagaaat tactggtaac
     1321 cgtaaagcct tttcagggaa caattccgtt ccgtgttttg cagcctggtc gtgttcttgt
     1381 tgaaggtacg ttcagtggta aatgtacgca atcatactcc cgggtctttc gggtggatgc
     1441 ctcgaatgaa gagctaaccg tggagtgtgc tgtgaatgcc gctaaatgcc gcatggtatc
     1501 ggctgcatta cagccagtgt gttgagcgac cttattatcc atggcgcggt attgtcgccg
//
LOCUS       DAZKJR010000349         1558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKJR010000349 DAZKJR010000000
VERSION     DAZKJR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.351"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..105)
                     /locus_tag="ACHOWS_005328"
     CDS             complement(<1..105)
                     /locus_tag="ACHOWS_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050735.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVF"
     gene            complement(89..>1558)
                     /locus_tag="ACHOWS_005329"
     CDS             complement(89..>1558)
                     /locus_tag="ACHOWS_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFQ0050736.1"
                     /translation="NRLPDPELHPDSTLTVWPDNRITEDAHYLYRYDEYGRLAEKTDR
                     IPAGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRTGKRVWRRG
                     RDLTGWMSLSRKPEETWYGWDGDRLTTVQADTTRIQTVYQPGSFTPLIRIETDVTELE
                     KAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRTDRVSSESRAWLAQCGLT
                     VEQLEKQVEPEYTPARKVHLYHCDHRGLPLALISEDGNTAWSGEYDEWGNLLNEENPH
                     HLHQPYRLPGQQHDEESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQV
                     IDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADA
                     MRHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSA
                     KCSDACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          317 a          472 c          364 g          405 t
ORIGIN      
        1 aaatacgatg tcagaatttg aattgttatc aaacccaagg taaacaacaa ataaacaaag
       61 ggtaattaaa aaaatgacaa gtttgcctct aataatttga agcatctgaa ctcccttgct
      121 ttgcttttat tggattatct gcttttatac catctaaccc gaagagttgc ccagtgttat
      181 atttttcaat acatgcatcg ctacatttgg cagaaaaatc gccacaagca ataccgacag
      241 tgttattttt aagatccatg attctttctt tagctggctg accttgtcta tcccctgccg
      301 cctcatgatt tttacctatc acgtcagcta ttgttgatcc aaatgtctta gtcattcgac
      361 acatcaagta acaatgtctc attgcatcgg cggccccatt atgagctccg ccaacaatag
      421 catccatatc ataaccgaac tggtaagcac tgttagtaat gctgtttgca gcaatcaatc
      481 caggtacacc agatacggca taaattagtc cacctgatgt catattctca atcgcatcta
      541 accccattgg gtctatcact tgtatgggat tcaacggata ctgatacata ttccatccac
      601 ctctcaaccc aatcgggtcc ggggtgatat accgcccctg caacggatcg tagtaccggt
      661 gacggttata gtacagcccc gactcctcat catgctgctg ccctggcaga cggtacggct
      721 ggtgcaggtg atgcgggttt tcctcattca gcaggtttcc ccactcatca tactccccgc
      781 tccacgccgt attgccgtct tcgctgataa gcgccagcgg cagtccccgg tggtcgcagt
      841 ggtaaagatg aacttttcgc gccggtgtgt attccggctc cacctgtttt tccagttgct
      901 ccaccgtcag cccgcactgc gcaagccacg cccggctttc actgctcacg cggtctgtcc
      961 ggatttcttc ctccagcctg tccagcagcc gcaccagttc agccgggaac accacgccgt
     1021 gtccgtcctc gctcccttcc tgctggagct tctccgccag gctgcggcgc tgcgctttct
     1081 ccagttcggt cacgtctgtc tcgatgcgga tgagcggcgt gaagcttccc ggctggtata
     1141 ccgtctggat acgcgtggtg tcggcctgca ccgtcgtcag cctgtcaccg tcccagccgt
     1201 accacgtctc ctccggttta cgcgacagcg acatccatcc tgtcaggtca cgcccgcgac
     1261 gccacacccg tttacccgtc cggcggccca gcgggtcgta aaggtagcgg ctctcgacca
     1321 gtggctcgcc atgctgtatc cgcgtataga acaccaggcg gtgctggctg tcgtagtggt
     1381 agtggtgggt ccgctcgtcg tccgtccgta tcacacccgc cgggatgcgg tccgtcttct
     1441 ccgccagcct gccgtattca tcgtaacggt acaggtagtg cgcgtcttcg gtgattcggt
     1501 tatccggcca cactgtgagt gtactgtccg ggtgcagctc cgggtccggc agccggtt
//
LOCUS       DAZKJR010000350         1516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKJR010000350 DAZKJR010000000
VERSION     DAZKJR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.352"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..417
                     /locus_tag="ACHOWS_005330"
     CDS             1..417
                     /locus_tag="ACHOWS_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFQ0050737.1"
                     /translation="MTNGEPFWPDLDLAELQGSRTLPADLPDDTAAMALLAAVAEVNT
                     TLAPVVAHWKAQGHERAADVPGARLGDETQLVAQYKKAVYARAKADLMGEFATIGRRE
                     TYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            414..887
                     /locus_tag="ACHOWS_005331"
     CDS             414..887
                     /locus_tag="ACHOWS_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0050738.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMELFVSRSRE"
     gene            884..>1516
                     /locus_tag="ACHOWS_005332"
     CDS             884..>1516
                     /locus_tag="ACHOWS_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFQ0050739.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRHRLLWRIARHGIIAAA
                     RRHQRNQCAPDGTPWEPRKNKKKKAGKNTGKMLKKLPRLMAVREIPGQDAVKVYFRAV
                     RYGPTASASNVAWAHHNGATIRYHGDSFRHKKPGRSVPCSLKQAQALRAWGLELKSGK
                     RWRKATLSMIRRRVSNRQAGWLLARFERDDWENFRDLHSSDGPGIRQRNSP"
BASE COUNT          358 a          435 c          477 g          246 t
ORIGIN      
        1 gtgacaaacg gcgagccgtt ctggccggac cttgatctgg ctgagcttca ggggtcacgc
       61 acgcttccgg ctgatttacc cgacgacacg gcggccatgg cgctgcttgc cgcagtggcc
      121 gaggtgaaca ccacgctggc gccggttgtg gcgcactgga aggcgcaggg ccatgaacgc
      181 gccgcagacg taccgggggc caggctgggc gatgaaaccc agctggtcgc acagtacaaa
      241 aaggccgttt atgcccgtgc caaggccgac cttatgggcg agttcgccac cattggtcgc
      301 cgtgaaacct atccggggca ggaaagcgag gacacacgcg ccaccttact ggctgaagcc
      361 tcgctggtca tgcgtaacat gctggggcac agacgcgcgg gagtgcacat catatgaacc
      421 agctggaaag cctgaccgcc ttcattaccg aaaatctgcc gcctgacgcc atgcagttct
      481 tcatgagcag tcaggaagac gcgcagctta tccgcagtca gaaggcgctg ggtaacgacc
      541 agatccgcat gggcgtgatg acgtacaccg gcgtgattgc atgggataac ttcccgttcc
      601 gcaaatactc accggggctg gtttacgcgc tggtcatggc ctggctggaa gagcacgcgg
      661 acccgctgag ggaggaattc cgcccggagg atgtgaacgt cgacccgcag tttaacgatg
      721 aggattcgtg ccttctcgat atcacggtgg gactggctga cccgctgatc atccgggtgc
      781 aggaagacgg ccccgttccg tacaggggga aacgctggac actggttaac cctgaaatct
      841 ggacggcgga aaccatggag ctgtttgtca gcaggagccg cgaatgataa gcggccagct
      901 gaacccgcag cagctggcag aagcgcaggc gatgcttcag tcgctggatc tgcccccgaa
      961 aaaacgccat cgcctgttat ggcgtattgc ccggcacggc attatcgccg cagccagacg
     1021 ccatcagcgc aaccagtgcg caccagacgg cacgccgtgg gaaccacgga aaaataaaaa
     1081 aaagaaggca ggcaagaaca ccggaaagat gcttaaaaaa ctcccgcgcc ttatggcagt
     1141 gcgggagata ccgggacagg acgcggtgaa ggtgtatttc cgcgcagtca gatacggccc
     1201 gacagcgtcg gcgagtaacg tcgcgtgggc acatcacaac ggcgccacca tccgctatca
     1261 cggcgacagc ttccggcaca aaaaaccggg gcgttccgta ccgtgttccc tgaagcaggc
     1321 acaggcgctg agagcatggg ggcttgagct gaaaagcggc aagcgctggc gaaaagccac
     1381 cctgtcaatg atacggagaa gggtatctaa ccgacaggca ggctggctgc tggcaagatt
     1441 tgagcgcgac gactgggaaa attttcgcga ccttcacagc agtgatggtc cgggaatacg
     1501 acaacgtaac agccca
//
LOCUS       DAZKJR010000351         1492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKJR010000351 DAZKJR010000000
VERSION     DAZKJR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.353"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..795
                     /locus_tag="ACHOWS_005333"
     CDS             <1..795
                     /locus_tag="ACHOWS_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFQ0050740.1"
                     /translation="LLKETDGKQRYEAWLMWLCDTFSVESGYAGLSFALPYARERMFP
                     CEFALAQRFSGVMVDSLGTLEGGGAVIGLKGACWYTILGTPWLEKLGGAERVKHRLAK
                     TPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSPLLVKVNQVIKPVRFNESRSLHFYS
                     EYEGLQFDKESTMKWYRRFDEASALLDKEEMGKSCAPERIKCWTDEIAPHAGQWAAMV
                     NGTTEYIQTREGQKMPPFEDKYGKQHRACWKLLKRDDNGSVYTLPE"
     gene            821..1200
                     /locus_tag="ACHOWS_005334"
                     /pseudo
     CDS             821..1200
                     /locus_tag="ACHOWS_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
     gene            1197..>1492
                     /locus_tag="ACHOWS_005335"
     CDS             1197..>1492
                     /locus_tag="ACHOWS_005335"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFQ0050741.1"
                     /translation="MNSVSFSLAAQHLICSTKVTLFALVDGLQYERYFGESLSVQQPA
                     AVPLFDTWPDSRIAFAGPWVMEMNSIMDFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT          362 a          328 c          452 g          350 t
ORIGIN      
        1 ctgctgaaag aaactgatgg aaagcagcgt tatgaagcct ggctgatgtg gctgtgtgac
       61 accttttcgg tggaaagtgg ttatgcaggg ttgtcgtttg cacttcctta tgcccgtgag
      121 cgaatgttcc cctgtgaatt cgccctggcg cagcgttttt ccggtgtgat ggtggattca
      181 ctgggaacgc tggaaggcgg tggggcagtc attggtctga aaggtgcctg ctggtacacc
      241 attctcggta cgccgtggct ggaaaaactg gggggggcag agagagtaaa acatcgttta
      301 gctaaaacgc ctgaaatcag cctgctaccc tataacaatg gtgtcgtact taaagccggt
      361 gaactccccc cgccactggg agagatgaaa acagaaggtt tatcgccgct gctggtgaag
      421 gtgaaccagg tcattaagcc agtgcggttt aatgaatcaa gatctcttca tttctactct
      481 gaatatgaag gacttcaatt cgacaaagaa tccaccatga agtggtacag gcgctttgac
      541 gaagccagcg ccctgctcga taaagaagaa atggggaaat cttgcgcccc cgaacgcatt
      601 aaatgctgga ccgatgaaat agcgccccat gccggacaat gggccgcgat ggttaatggc
      661 accaccgagt acatccagac ccgcgaaggg cagaaaatgc ccccgtttga ggataagtac
      721 ggtaagcagc acagggcttg ctggaaacta ctgaaacgag atgacaatgg tagcgtttat
      781 acactacctg agtaaggtcg tgtgctgaat gaccgcgcag gccggagaga ggctggggct
      841 ttttgcccgg accggacaac tgagcgtcaa gtccggggag gggccggtag aggttcaggc
      901 gaaaaacggc agcatgcggt tgtttgctga gaagaagctg acactgagct cagcgtgtga
      961 tatctctttt gccgggaaaa aacgtatcac gctgattggc gggggagtta cctgcgactt
     1021 gaagccggga aggtcgagta tggtacgacg gcaaagtatc tgcgcaaagt gaagcggaca
     1081 atggcggcgg gggctgcatc aataccagtc aggtccattg tggggggagg tatttgcctg
     1141 agctgtctga tgaaggcggc aatgaatggc gacacctttg ttgtaagggg tgggtcatga
     1201 acagcgtcag ttttagcctt gcggcacaac acctgatttg ttcaacaaaa gtgacattat
     1261 ttgctctggt ggatggtctg caatatgagc ggtatttcgg tgagagtctg tctgttcaac
     1321 aacctgccgc cgtgcccctg ttcgatacct ggcctgattc gcgtattgca tttgcggggc
     1381 catgggttat ggagatgaac agcataatgg atttcagaga aaggctgtgt gagcttgaag
     1441 ctgcgcttcc gtcagtttcc tggatgatat cttcttcctc gcttactgaa ct
//
LOCUS       DAZKJR010000352         1481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKJR010000352 DAZKJR010000000
VERSION     DAZKJR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.354"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..1462
                     /gene="gndA"
                     /locus_tag="ACHOWS_005336"
     CDS             56..1462
                     /gene="gndA"
                     /locus_tag="ACHOWS_005336"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0050742.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIV
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          375 a          356 c          375 g          375 t
ORIGIN      
        1 ctttttaagt tattctcata ccgcacatgc ggtaaaaccc tgacaggagt aaacaatgtc
       61 aaagcaacag atcggcgtcg tcggtatggc agtgatgggg cgcaacctcg cgctcaacat
      121 cgaaagtcgt ggttataccg tctctatttt caaccgttcc cgtgaaaaga cggaagaagt
      181 gattgtcgaa aatccaggca agaaactggt tccttactat acggtgaaag agtttgttga
      241 atctctggaa acgcctcgtc gcatcctgtt aatggtgaaa gcaggtgcag gcacggatgc
      301 tgctattgat tccctcaagc catacctcga taaaggtgac atcatcattg atggtggtaa
      361 taccttcttc caggacacca ttcgtcgtaa ccgtgagctt tctgccgaag gctttaattt
      421 tatcggtacc ggtgtttccg gtggtgaaga aggtgcgctg aaaggtcctt ccattatgcc
      481 tggtgggcag aaagaagcct atgaactggt tgcaccgatc ctgaccaaaa tcgccgcagt
      541 ggctgaagac ggtgagccat gcgttaccta tattggtgcc gatggtgcag gtcactatgt
      601 gaagatggtt cacaacggta ttgaatacgg cgatatgcag ctgattgctg aagcctattc
      661 tctgcttaaa ggtggcctga atctcaccaa cgaagaactg gcgcagacgt ttaccgagtg
      721 gaataacggt gaactaagca gctacctgat cgacatcacc aaagatatct tcaccaaaaa
      781 agatgaagac ggtaactacc tggttgatgt gattctggat gaagcggcta acaaaggtac
      841 cggtaaatgg accagccaga gtgcgctgga tctcggcgaa ccgctgtcgc tgattaccga
      901 gtctgtgttt gcacgttata tctcttctct gaaagatcag cgcgttgccg catctaaagt
      961 tctctctggt ccgcaagctc agtcagcagg cgacaaggct gagttcatcg aaaaagttcg
     1021 tcgtgcgctg tatctgggca aaatcgtttc ttacgctcag ggcttctctc agctacgcgc
     1081 agcgtctgaa gagtacaact gggatctgaa ctatggcgaa atcgcgaaga ttttccgtgc
     1141 tggctgcatt atccgtgcgc agttcctgca gaaaatcacc gatgcttatg ccgaaaatcc
     1201 gcagatcgct aacctgctgc tggctcctta cttcaagcaa attgccgatg actaccagca
     1261 ggcgctgcgc gatgtcgttg cttatgcagt acagaacggt atcccggttc cgaccttcgc
     1321 cgctgcggtt gcctattatg acagctaccg tgccgctgtt ctgcctgcga acctgatcca
     1381 ggcacagcgt gactatttcg gtgcgcatac ttataagcgc attgataaag aaggtgtgtt
     1441 ccataccgaa tggctggatt aatctgattt aatcaacaat a
//
LOCUS       DAZKJR010000353         1481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKJR010000353 DAZKJR010000000
VERSION     DAZKJR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.355"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(176..1420)
                     /locus_tag="ACHOWS_005337"
     CDS             complement(176..1420)
                     /locus_tag="ACHOWS_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFQ0050743.1"
                     /translation="MSKKFTKTLLSSSLVVGLLSVSSFTSASEYSYTALDIVGRHGTT
                     YTIVYNSDSDTAYVKWKTVPAGAEGIESGSIVQGLSKINRDGTVEVTTKDGTSTYRVY
                     DREFHDLMNSLVKQKREGSLGKNEGTKTELVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGA
                     TKTIAVNDGLVALSGRTDRIDATVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTA
                     AKAGVAASDGDVSYNVGVNFEF"
BASE COUNT          376 a          406 c          273 g          426 t
ORIGIN      
        1 tccctcgcca gtattatcct gtacccctca agcatctgct gcaggccata ctccagataa
       61 cggttgcggg tcgccagaat cactttcatc gctgtcacct gtttcatgct cacatgagaa
      121 aaaacggcag gcaaaactcc acgcctgccg tcatgcttca tgtcactgtc agcaattaaa
      181 actcaaagtt cacacctacg ttgtaggaca catcgccatc actcgctgcc acacctgctt
      241 tcgctgctgt ctgctcattg aaacggtaac caacacccac ggcaacagcc tgtttatcac
      301 tgtacccccc cagggcagca gtggcgttga atttccccac actgtacggc tggaaaagcc
      361 ccgccagcgc tgcactctgt gctgctgcac gcttcatctc ttcgtgattc tcacgaattt
      421 gacgctgctg gctgttcagg cgtgcattat gttcctgcaa ctggcgagta ttggactgaa
      481 tagcctgtgt atttttagta acccggcggt caacgctacc aacagtagca tcaatacggt
      541 ctgttctgcc ggacagagca acgagaccgt cattaacagc aatcgttttt gtagcgccat
      601 cagcgctggt caaggttccg tccgcattca caccaacggt ggttcgggaa tcaattttat
      661 tcgtattcgc agccgttgct gcatcaatga tatctgtgcg ggatgaaagc tcagacagtc
      721 ctttcttctg gatcgccagc ccttctttct gttcttccac atctttcgct atagtgacaa
      781 cgccgtcatt gatacgtaac ggcgttacaa tatctgtctc attcagagtt agcttaccgt
      841 ttttatctag gccaattgcg ctatttgcag cctctgtagc cagataactc tgcaaagaca
      901 cgcgttcttt aatactctct gcactagttg ccactcgccc atattcatcc agaccaacat
      961 tagtttccat ttcagagatc gcagcaattc tgccaaaaag attccgaata tcagcatcag
     1021 atacaagctc tgttttagta ccctcgttct ttccaagaga accttctctt ttctgcttga
     1081 ccagtgaatt cattaggtca tgaaactccc ggtcatacac acgatatgta ctagtgccat
     1141 ccttggtagt aacttctaca gtcccatctc tattaatttt tgataatcct tgtacaatac
     1201 ttccactttc aatcccttca gcaccggcag gtactgtttt ccatttaaca taagcggtgt
     1261 cagaatcact attataaaca atggtgtaag tagttccgtg tctaccgaca atatccaagg
     1321 cagtgtagct atattcactt gcacttgtaa aagaactaac actcaacaga cctacaacca
     1381 gagaagatga cagaagtgtc tttgtaaatt ttttactcat tatggatatc ctgttaaatt
     1441 atctgtatac aaatcagcca ctacacctca tactcataaa t
//
LOCUS       DAZKJR010000354         1453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKJR010000354 DAZKJR010000000
VERSION     DAZKJR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.356"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHOWS_005338"
     CDS             complement(<1..1001)
                     /locus_tag="ACHOWS_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFQ0050744.1"
                     /translation="MRSFLTSKAGSVVQQFSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYLVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(986..>1453)
                     /locus_tag="ACHOWS_005339"
     CDS             complement(986..>1453)
                     /locus_tag="ACHOWS_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFQ0050745.1"
                     /translation="SDLLMQLLDEQPEATEASLHELLRPILRDHFQPALLARFRTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLAWSDEDGIVLVFDAQAEGESSCAVS"
BASE COUNT          322 a          432 c          391 g          308 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccgctgaca
       61 agatactgcg cattcagaat acgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgc cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgtccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgctc aagacggctg
      421 tccatttcat aacgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagctcc cggggtggat actcccggga gagccggaac
      541 tcaaaatcct gcccttccag accatgttta cgcaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcaat acggggaacg
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcagaaa tgcgacgaaa cgactcaacc
      721 acgccgaata ccgttctgag cggcagggcc ggtgttccgt cagacaccgg ggtctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga ggagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaaactgc
      961 tgtacaacgc tgccagcctt gctggtcagg aaactgcgca tgacgactct ccctctgcct
     1021 gtgcatcaaa taccagtaca atgccatcct cgtcactcca tgccagagtc agcgcctgcg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagctgct
     1141 ggttcagcag actctccaca ttccgggcac ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta cacacgttca tcaaccgatg tgctcagccc ataatggcag tgcaggcgtt
     1261 cgcagacctg tgcaagcttc atctccacaa tggtgcgcat ggcagactgc gtcagcggac
     1321 ggtaaatcac ggtccggaaa cgggcgagca gtgcaggctg gaagtggtcg cgcagtatcg
     1381 ggcgcagcag ttcgtgcagg ctggcctcag tggcttccgg ttgttcgtcc agcaactgca
     1441 tcagaaggtc act
//
LOCUS       DAZKJR010000355         1453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKJR010000355 DAZKJR010000000
VERSION     DAZKJR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.357"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..486
                     /locus_tag="ACHOWS_005340"
     CDS             52..486
                     /locus_tag="ACHOWS_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFQ0050746.1"
                     /translation="MNDNALVNVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKDGLLSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            490..804
                     /locus_tag="ACHOWS_005341"
     CDS             490..804
                     /locus_tag="ACHOWS_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050747.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKEELWYYNLFINPSADELMNVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            888..>1453
                     /locus_tag="ACHOWS_005342"
     CDS             888..>1453
                     /locus_tag="ACHOWS_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFQ0050748.1"
                     /translation="MSEWRKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKII
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLL"
BASE COUNT          356 a          318 c          424 g          355 t
ORIGIN      
        1 cctcacgggg cagtgctctc tgttatttca ggattacaga ggaactgagt gatgaacgat
       61 aacgcactgg ttaatgttga cgatgctgtt gtggcccgta tcagggatgt gctggcgtat
      121 acatttccgg gacagaaatt ttctgtgcgg aaatgcgatc catctttccc gaaagttttc
      181 aacaatgtgt gggtgagctg gctggatggt ccaacggagg ccgaagtacg ctctgtcaca
      241 gaggggtttg agaataaaga cgggctgctg tctgtgcttg aatcccgtta tttcagcgat
      301 gaactggtac aggagtgtat tgatcgcctt cgtaaagagt acggacaggg gaacgtcccg
      361 ttgagtgtga ccgtggagcg ttaccggaaa aacgagctgg agaagattga aacagcgcgt
      421 tttccgggca acatatccgt agccataaac gagatcggga taaataccag caaatacagg
      481 aggtaaggta tggctggtaa atgccttgtc tggttctgta acgggatctc tgaaccgtgt
      541 ctgatagcag gaaatatcag tgaattactg gcagatgtta cgatcacgtc aaattaccgt
      601 ggagatgtca catctgaaat cagggatgtt gtacaggagt ggctcaggac cggcaggggg
      661 aacctggcgg atttaaagga agagctctgg tactacaacc ttttcatcaa tccgtctgca
      721 gacgaactga tgaatgtcaa caggcggtat ggccctgagc gaactgcctg gctgcagggt
      781 tttatccgca acaacggggc gtgatacccc gttttcccgt gagggcattg tacctcacgg
      841 gacaatgccc tctttttaca tctgtataaa aaagaggagc gtaaacaatg tctgaatggc
      901 gcaaaaaccg ttttgaaatt accggtaaat cagtctgtct tgatgtactt acacagtgga
      961 ttgaagggcg tgagaccccg cgctaccgtc atgccataca gcagagtata cagctgtttc
     1021 tggccggttg tgcagggctg gtaaaaccca cacggacgac gcagtatccg ccatatccgg
     1081 cgctggttcg tcacggaacg gggttgtcat ctccggctaa ccaggcgttt gagcagtggc
     1141 tggggttgct ggtaaaaaat gccgttctgg atggtgaaac gataaagatc attgagcgtc
     1201 tttatcacca gtcatgtatc ggcgctgttc gctggggtaa tctgccggat aatgccaggg
     1261 agatcatcac cacgctgatg agccgtcagt attctgactg gtttggtctg gccgggctga
     1321 gtgaacacat tgatgcagaa gcctgctgga gctggttgtc ggattacccg gaacaggcgc
     1381 agccctgtga catgctgatg gtcataccct cccgtctggc tacagagctg aatggttcag
     1441 gtggactgct gca
//
LOCUS       DAZKJR010000356         1440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKJR010000356 DAZKJR010000000
VERSION     DAZKJR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.359"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1301
                     /locus_tag="ACHOWS_005343"
     CDS             <1..1301
                     /locus_tag="ACHOWS_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFQ0050749.1"
                     /translation="TGASESSTRWGVGRPLYKDLIGRTKAALAKNPKNVLLAVVWMQG
                     EFDFNGTPANHAARFTEVVDQYRADLADMAGQCVGGSADSVPWICGDTTYFWKQKNET
                     AYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPDIGYYGSKWRTDRST
                     WTSQDRASHFSSWARRGIISDRLATAILSCAGKSSAFVNGTAGVVVPDRPVTTSESVI
                     FYDAKKASDNQLKPYGWDGMDGRRTLVDDSGNKALRIEKNNSAKSWSMYCDIAADKAK
                     LLLEKGGEIAVRFKIPENVNLETTRNKYAFGLYWRIAEWPGEGGEGYLSSFFVQTDKA
                     SIDVAYHHTVNQQKELGTFGAFDHDWHTLAFKFKGSNSINVTPVLDGVDGQAFDLVKW
                     ANTANGLNRFVITDITGSAETYPVLIDTVEVKANKAGAAA"
BASE COUNT          412 a          289 c          396 g          343 t
ORIGIN      
        1 ctactggcgc ctcagagagt tccacccgct ggggggtggg caggccgctg tataaggacc
       61 tcattggtcg tacaaaagcc gcgctggcga agaatccgaa aaatgtgctg cttgccgtgg
      121 tatggatgca aggggaattt gattttaacg gaacgccagc gaatcatgca gcccgtttta
      181 cagaagtggt ggatcaatac cgtgcggacc ttgcagatat ggcggggcag tgtgtgggtg
      241 gctcagctga cagtgttccc tggatttgtg gagacacaac atatttctgg aagcagaaga
      301 acgaaacggc gtaccagacg gtgtatggta gttacaaaaa taaaacggaa aagaatatcc
      361 atttcgtgcc gttcatgacc gatgagaacg gggtgaatgt gccgacgaac aaaccggaag
      421 aagacccgga tattccggat atcgggtatt acggttcaaa atggcgaacg gacagaagca
      481 cctggacatc tcaggacagg gccagtcatt tcagttcatg ggctcgccgt gggattattt
      541 ccgaccgtct ggcaacggcg attctgagct gcgcgggtaa gtcttctgcg tttgttaatg
      601 gtactgccgg ggtggttgtt ccagacagac cggttaccac ctcagagtct gtaatttttt
      661 acgatgccaa aaaagcttca gacaatcagc tgaaacctta tggctgggac ggtatggatg
      721 gcagacgcac actggttgat gacagcggca ataaagctct gcgaattgag aaaaataaca
      781 gcgcgaaatc ctggtcaatg tactgtgata ttgctgcaga caaggcaaaa cttttactgg
      841 aaaaaggcgg ggaaattgct gtccggttta aaatccccga aaacgtcaat cttgagacaa
      901 ccagaaacaa gtatgccttt ggtttgtact ggcgaatagc ggaatggccg ggtgagggtg
      961 gtgaaggcta tctgagttct ttctttgtcc agacagataa agccagtatt gatgttgcat
     1021 accatcatac agttaatcaa caaaaagaac ttggcacgtt tggcgcattc gaccatgact
     1081 ggcatacgct tgcatttaaa tttaagggca gtaacagcat taatgttact ccggtgcttg
     1141 atggtgtgga tggacaggcg tttgacctgg tgaaatgggc caatactgct aatggactca
     1201 acaggtttgt cattacggat attacaggta gtgcagaaac ctaccctgta cttattgata
     1261 cggtggaagt taaagcaaac aaagctggag cagccgcata attgctaaaa aaagccgcca
     1321 gcggcaggaa tggaagctgg cggaggtaat cccaatggag aatgtaaaga aaagatgctt
     1381 tcgtacattg gttttttaaa tgaaaacagt tctcattgtc aaccataacg gtaagaaatt
//
LOCUS       DAZKJR010000357         1431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKJR010000357 DAZKJR010000000
VERSION     DAZKJR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.360"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(127..1398)
                     /gene="dcuC"
                     /locus_tag="ACHOWS_005344"
     CDS             complement(127..1398)
                     /gene="dcuC"
                     /locus_tag="ACHOWS_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFQ0050750.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYLNPGVAHNS
                     FISKISQTPLMEFVVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVEF
                     TDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKEL
                     VSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGPF
                     LMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATIIC
                     AGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
BASE COUNT          465 a          309 c          286 g          371 t
ORIGIN      
        1 cataccacgc agaatagtaa ttatctttga cgttgtgata aattcgcacc acccactttc
       61 cggaatatgg tgaaattctc tccgccattt ttgtaactga ttcagttcag acatatacgc
      121 ctcctgttaa aggaataata atgcaataaa caataccgtc acaatcattc cgggggcagt
      181 gcgttttgca atttcaactg gattaactcc agccagaccg gcacagataa tagttgcacc
      241 agccaacgga gacatgtttc tccctagcgc agaagccagc ataactgcag cacccagagt
      301 atttgtatcg taaccaaaat tgttcgcatg tggtgtgaca acttcgttga atgcgaatgc
      361 agtcgcatcc cctgtgccgg ttataagtcc catcaggaat ggccccaatg taccgcccca
      421 gcgggcaaat tctggtgagt cagtaagaaa tctgataaac acatcaatga taccaacaga
      481 tcttaaacct tcaatgaaaa cagctgcgga aattatgatc ccaagaatat ttgcatatga
      541 tgtccccata ccctcaaaga agctggaaac taattctttg ggattggatt tactgacaat
      601 cagggcataa agacaaccga gaatcattgc ggctggaacg gttattttag tctctccaag
      661 aaaaccaaat tcagccagca taagtaatgc tacgggaaga aatggtgcta cagatttcag
      721 ataaaaacag atatcacggt cagtgaactc aacctgtgtt tgttctgata tgtttcctgc
      781 agaatcacat tttgtttctt ttcttacaat agcaacaact gtaagaataa cagcacatat
      841 tacaccacat gttattgtag tcggagtatg gaatacgacg aattccatta atggtgtttg
      901 tgatatttta gatataaatg aattatgtgc aacaccggga ttgaggtaag caccgatagt
      961 tcctgaaagg atagctgctc cggctattgc aggatgaata cgtgctgaaa taagtaatgg
     1021 tatcagcgtt gcaccaacag cagctgatgc tccagcagca gatggaatcg cgatgcaaac
     1081 aataaaactg acggtaaccg cagcaggaat aagaaagata ccaagacgag ataatccttt
     1141 cgttagaaaa tgaaccaggc atgtatcaca accagtgaat ttcataacac aggcaaagcc
     1201 catactggta caaatggcct gaatgagacc ggactcagtc attctgcttg caaatgccga
     1261 aaatgcgttt aaaggtgaca tggcaactat acatagcaga atacctgcac caattagtgt
     1321 cgttcgtgtg tcgtatcttt ttatcaaaag cacaactgtg attatcagaa tcacacatcc
     1381 agttatcacg ttaaacatga gatcctcctt cttcaaagaa ggataatctt t
//
LOCUS       DAZKJR010000358         1426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKJR010000358 DAZKJR010000000
VERSION     DAZKJR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.361"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..381
                     /locus_tag="ACHOWS_005345"
     CDS             <1..381
                     /locus_tag="ACHOWS_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFQ0050751.1"
                     /translation="LAELQGSRTLPADLPDDTAAMALLAAVDEVNTTLAPVVAHWKAQ
                     GHERAADVPGARMGDETQLVAQYKKAVYARAKADLMGEFATIGRRETYPGQESEDTRA
                     TLLAEASLVMRNMLGHRRAGVHII"
     gene            378..851
                     /locus_tag="ACHOWS_005346"
     CDS             378..851
                     /locus_tag="ACHOWS_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0050752.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMELFVSRSRE"
     gene            848..>1426
                     /locus_tag="ACHOWS_005347"
     CDS             848..>1426
                     /locus_tag="ACHOWS_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050753.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQTAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQNGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITIMEDQIIAGPRQQKA"
BASE COUNT          321 a          412 c          456 g          237 t
ORIGIN      
        1 ctggctgagc ttcaggggtc acgcacgctt ccggctgatt tacccgacga cacggcggcc
       61 atggcgctgc ttgccgcagt ggacgaggtg aacaccacgc tggcgccggt tgtggcgcac
      121 tggaaggcgc aggggcatga acgcgccgca gacgtaccgg gggccaggat gggcgatgaa
      181 acccagctgg tcgcacagta caaaaaggcc gtttatgccc gtgccaaggc cgatcttatg
      241 ggcgagttcg ccaccattgg tcgccgtgaa acctatccgg ggcaggaaag cgaggacaca
      301 cgcgccacct tactggctga agcctcgctg gtcatgcgta acatgctggg gcacagacgc
      361 gcaggagtgc acatcatatg aaccagctgg aaagcctgac cgccttcatt accgaaaatc
      421 tgccgcctga cgccatgcag ttcttcatga gcagtcagga agacgcgcag cttatccgca
      481 gtcagaaggc gctgggtaac gaccagatcc gcatgggcgt gatgacgtac accggcgtga
      541 ttgcatggga taacttcccg ttccgtaaat actcaccggg gctggtttac gcgctggtca
      601 tggcctggct ggaagagcac gcggacccac tgagggagga attccgcccg gaggatgtga
      661 acgtcgaccc gcagtttaac gatgaggact cgtgccttct cgatatcacg gtgggactgg
      721 ctgacccgct gatcatccgg gtgcaggaag acggccccgt tccgtacagg gggaaacgct
      781 ggacactggt taaccctgaa atctggacgg cggaaaccat ggagctgttt gtcagcagga
      841 gccgcgaatg ataagcggcc agctgaaccc gcagcagctg gcagaagcgc aggcgatgct
      901 tcagtcgctg gatctgccac caaaaaaacg ccgtcgcctg ttatggcgta ttgcccggca
      961 cggcattatc gccgcagcca gacgccatca gcgcgatcag accgcgccgg acggcacagc
     1021 atgggcaccc cggaaacgcg gcaaggggaa aatgttcaga aagctgccgc gtcttctggc
     1081 ggtccgtgag atgcccggac aggatgccgt aaaggtttat ttccgtaacg gcaatcacac
     1141 cagtggcagg agccgcatca gtgccggtgc cgtgggctgg gttcaccaca acggcgcaga
     1201 aatccgggtc agtgctgaca gcttcagcgg tcaggaccag aacgggcaga ccgccacccg
     1261 ccgacaggca aaacgactgc gggagctggg ctttaaggta cgccagaagg ggcgctggcg
     1321 gaaagcatcc atgaaatacc tgttaacgct ttcgcgggcg caggccgggt tgctgatcac
     1381 cattatggaa gatcagatta tcgccggacc acgccagcag aaggcc
//
LOCUS       DAZKJR010000359         1419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKJR010000359 DAZKJR010000000
VERSION     DAZKJR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.362"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1124)
                     /locus_tag="ACHOWS_005348"
     CDS             complement(<1..1124)
                     /locus_tag="ACHOWS_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFQ0050754.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDGSSWASPDTASASETDST
                     AVGAWANAGKAGTALGSASYAHAPFSTSVGMSAYVNPEASNGVAVGSWASADALNSVA
                     LGTGAQAMHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGLLSQRDVLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQ
                     EMATENRAAVSRNRQVGERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQ
                     SAALAGLFQPYSVGKFNATAAVGGYSDKQAVAVGMGYRFN"
BASE COUNT          369 a          407 c          303 g          340 t
ORIGIN      
        1 tcgttaaagc gatatcccat gcccaccgca acagcctgct tgtcactgta cccgcccaca
       61 gccgctgtgg cgttaaattt acccacactg tatggctgga aaagaccggc cagcgctgct
      121 gactgggctg ctgcccgctt catttcctca tgattctcat taatctgacg ctgctgactg
      181 ttcatacgac gctcatgcgc ctgcagggta cgtgcctgag aagcaatggc gcgactgttg
      241 cgttcaccca cctgacggtt acggcttacc gcagcacggt tttctgtggc catttcctgt
      301 gtacgtacca gcccttcctg tacagtaatt cgttcaccgg ttccctcacc gcgcgtcaca
      361 taaccatgac tgtccacagc aacggtactt ccggtattta tctgctgttg ctggctgttc
      421 aggcgctctt catgcgtctc cagcacgtct ctctgggaaa gcagccccct ggtgttacgg
      481 tttatggcca ccgtgttccg gataatattc tccctgttct ctgcagcaac cgtatcggta
      541 tggtcacgcg ccttaagtgc ttccgctgca atccgtccac tgacgtcact gtacacctca
      601 tccgccttcc ggttcagttg cccgacagtg gctgcatccg tatcccgtgt accatcactg
      661 acaccaccga gaacccgggt tttatccggt acacgcggac tggccgggtt tgtgacacct
      721 gagtcatatc cgatgtagac ttcattttca cgggttgtac cggactgctg accaagagcc
      781 actgaatacc tgtgcattgc ctgtgcaccc gtacccagtg ctactgaatt taaagcatct
      841 gcagatgccc agctacctac tgccacacca ttagacgcct ccggatttac ataagcagac
      901 atccccacgg atgtggaaaa cggcgcatgc gcatacgatg cagagccaag agctgtccct
      961 gccttcccgg cattcgccca tgcccccact gccgtggagt cagtctctga tgcagaggct
     1021 gtgtccggag acgcccagga actgccatcc tgcgcaaacg ccggagcacc ggcaatcaga
     1081 aaggaaataa gcgataaagt aataccgtgt cgatttattt tcataaacaa cctcattgat
     1141 atacacaata tactggcaca gagttaaatt aatatataat caggagagaa gtgaaagagt
     1201 acccggtgcg aaaatgaatt aatctgagca gatttaacgg taaacgaagc gcaataaatt
     1261 aaaattaaaa aacaaaatca cttgcagtct attatcttta cgttcctcac cgcaaccgca
     1321 cacaaaaaca aaacacacaa tgcattgatt ttaaacaaaa aaacaaaaca gaaactcaac
     1381 acataaaaat cagacaatat aaacagaatt gtgatttat
//
LOCUS       DAZKJR010000360         1404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKJR010000360 DAZKJR010000000
VERSION     DAZKJR010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.363"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..332
                     /locus_tag="ACHOWS_005349"
     CDS             <1..332
                     /locus_tag="ACHOWS_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFQ0050755.1"
                     /translation="IPGFHFTGTRQALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTM
                     TQWADKIGHLQIADNPHRGEPGTGEINYEYLFTVIENSDYNGWVGCEYKPQTTTEAGL
                     RWMDPYR"
     gene            432..1310
                     /gene="glxR"
                     /locus_tag="ACHOWS_005350"
     CDS             432..1310
                     /gene="glxR"
                     /locus_tag="ACHOWS_005350"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFQ0050756.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
BASE COUNT          385 a          303 c          360 g          356 t
ORIGIN      
        1 atattcctgg tttccatttc accggaaccc ggcaggcact gaaactgatt gacgatgttg
       61 gttgctgcaa tttgaaaatt cagtatgaca tttatcacat gcagcggatg gaaggtgaat
      121 taactaatac catgactcag tgggccgata aaattggtca cctgcaaatt gctgataatc
      181 cgcatcgcgg cgaaccggga accggagaaa ttaattacga atatctcttt acggtaattg
      241 aaaattctga ctacaacggt tgggttggct gtgaatataa accccaaacc accacggaag
      301 ccggtttacg ctggatggat ccgtaccgtt aaaacgtaac gctattcaga caatgctttt
      361 ttaggccgct aagttggcag gggatcgtgt tgtctgaatt caggaaaagc gaaatttaaa
      421 agaggttaat tatgaaactg ggatttattg gcttaggcat tatgggtaca ccgatggcca
      481 ttaatctggc gcgtgccggt catcaactgc atgtcacaac cattggtccg gttgctgatg
      541 aattactgtc actgggcgcc gttagtgttg aaactgcgcg ccaggtaaca gatgcatcgg
      601 acatcatttt tattatggtg ccggatacgc cacaggttga agacgttctg tttggtgaaa
      661 atggttgtac caaagcctcg ctgaagggca aaaccattgt tgatatgagc tccatttccc
      721 cgattgaaac taaacgtttc gctcgtcagg tgaatgaact gggcggcgat tatctcgatg
      781 cgccagtctc cggcggtgaa atcggcgcgc gtgaagggac gttatcgatt atggttggcg
      841 gtgatgaagc agtatttgaa cgtgtaaaac cgctgtttga actgctcggt aaaaatatca
      901 ctctcgtggg cggtaacggc gacggtcaaa cctgcaaagt ggcaaatcag attatcgtgg
      961 cgctcaatat tgaagcggtt tctgaagcct tgttgtttgc ttcaaaagcc ggtgcggacc
     1021 cggtacgtgt gcgccaggcg ctgatgggcg gctttgcttc ctcacgtatt ctggaagttc
     1081 acggtgagcg catgattaaa cgcaccttta atccaggttt caaaattgcg ctgcaccaga
     1141 aagatctcaa cctggcactg caaagtgcga aagcactggc gctgaacctg ccaaacaccg
     1201 cgacctgcca ggaattgttt aatacctgtg cggcaaacgg tggcagccag ttggatcact
     1261 ctgcattagt gcaggcgctg gaattaatgg ctaaccataa actggcctga tacccgcaat
     1321 aaaaatggcc gatatcagaa aatgaatcgg ccagcaatat taaaaaaaga aagcagccaa
     1381 agatgttgct tcagtattaa aaat
//
LOCUS       DAZKJR010000361         1402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKJR010000361 DAZKJR010000000
VERSION     DAZKJR010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.364"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1006
                     /locus_tag="ACHOWS_005351"
     CDS             <1..1006
                     /locus_tag="ACHOWS_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFQ0050757.1"
                     /translation="YVKGGVMYRYDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLD
                     KPESRVKQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCV
                     GQGLHIAKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTA
                     LYEDLVARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSE
                     YSSQCVGNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRH
                     ELTNEPEEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL
                     "
     gene            complement(1321..>1402)
                     /locus_tag="ACHOWS_005352"
     CDS             complement(1321..>1402)
                     /locus_tag="ACHOWS_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFQ0050758.1"
                     /translation="LTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          406 a          264 c          350 g          382 t
ORIGIN      
        1 ttatgtaaag gggggagtga tgtatcgtta tgattttaat gaaaaaatag atttctggta
       61 tgtgatagcc ctcgcaggac agtctaatgg tatggcctac ggtgaaggta ttcccctgcc
      121 agatacactc gacaaacccg agtcccgtgt aaaacaactg gctcgtcgaa aaacaatcac
      181 accaggagga aaagagtgta aattcaatga gattattccg gcagatcact gtctgcatga
      241 tgttcaggat atgagtggat atcaccatcc ggctgctgat ttgcataaag gggaatatgg
      301 ttgtgtcgga cagggattgc atatagctaa aaagttactg ccatacatac ctgagcaagc
      361 agggatatta ctggttcctt gttgcagagg aggtgccgcc tttactgttg gagctgaagg
      421 tatgtatgta cctgatacag gggcaacagc tgatgcaatg cggtggggca ccggaacagc
      481 actctatgag gacttagtag cccgggttaa ggtagctctg gaatataaca gaaagaataa
      541 actgctatct gtctgctgga tgcaggggga gtttgatctt atgtcaccag attatgaaaa
      601 gcatcctgac ttgttttatc agatggtaac ttctttcagg tctgagttat ctgaatactc
      661 atctcagtgt gttggtaatt cgtcagaaag agttccatgg ttgtgtggtg atacaacctg
      721 gtactggaag gaatcttatc aaaaagaata tgattttata tatggacatt acagacaacg
      781 gactgatgat gaaatacatt ttctcagctt tcaggatagt aaccgacatg aattaactaa
      841 tgagccagaa gaagatgccg atgatttatc tgtcgggtat ctggggtcat catggaggac
      901 agaattaagc tggacaacgt cacagagaag tacacacttt aattcaatgg ccagaagggg
      961 agttattgct gaatgttatg cgcaaaaaat aagaaattat ttgtagacaa gttcaggata
     1021 tatctacagc tctgacatga ttctctgaga gacatgacgg agtggtatgg agttgtcggt
     1081 atcaacacca agatagctgg tcgttaccgt ttgatatcac tggttctgat actatgtgga
     1141 cggtagtgtg tcacgaaggc acgcaccagg gacgtgagtc cctgtaatat catcgacagt
     1201 cccattatgg catgtctgca cctacaacgt catttcgagg tcggctcagt gttcactcga
     1261 gtcttatatc actgacgccg acactccatc cagagtatct ctggacggaa acatcaccta
     1321 ttagttcttt tttctgtcag ggccaaaaca ttcagtcgat gtggcgcagg tcgataaccc
     1381 aacaagggca ttggacgtta at
//
LOCUS       DAZKJR010000362         1379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKJR010000362 DAZKJR010000000
VERSION     DAZKJR010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.365"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            160..774
                     /gene="evgA"
                     /locus_tag="ACHOWS_005353"
     CDS             160..774
                     /gene="evgA"
                     /locus_tag="ACHOWS_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFQ0050759.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            779..>1379
                     /locus_tag="ACHOWS_005354"
     CDS             779..>1379
                     /locus_tag="ACHOWS_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0050760.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALAS"
BASE COUNT          434 a          276 c          267 g          402 t
ORIGIN      
        1 ttgacatttc atttatgccg actatttata tggtatactt gttgaattat cttaaaggaa
       61 gctcaaattt tcttattttg attgagaaaa tgagatgatg ccttatgtct gtattactac
      121 agggagaagg gaaatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat
      181 gaccatcctc ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc
      241 ttagcggagt tgactgaagg gggaagtgcc gttcagcggg tggaaacact taaacctgat
      301 atcgtcatca ttgatgtcga tatccccgga gttaacggta tccaggtgtt agaaacgctg
      361 aggaagcgcc aatatagcgg aattattatt atcgtctccg ctaaaaatga ccatttttac
      421 gggaaacatt gtgctgatgc tggcgctaat ggtttcgtga gtaaaaaaga aggcatgaac
      481 aatatcattg cagctattga agctgcaaaa aatggctact gctatttccc cttctctctc
      541 aaccggtttg ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa
      601 gaaattagtg tcatgcggta tattcttgat ggcaaggata ataatgacat tgctgaaaaa
      661 atgttcatca gcaacaaaac tgtcagcact tataaaagtc gcttgatgga aaaattagaa
      721 tgcaaatcac tgatggatct ttacacattc gcacaacgta acaaaatcgg ctaaccacat
      781 gaagttttta ccctatattt ttcttctctg ttgtggtctt tggtcgacca taagtttcgc
      841 agacggagat tacatcgaat atcgtggcat cagtagtaac aaccgtgtca cacttgatcc
      901 actacgtctg agcaacaagg aattacgttg gctcgcgagc aaaaaaaatc ttgtgattgc
      961 agtacacaag tcccaaacag ctacgttgtt gcataccgat tcgcagcaac ggattcgtgg
     1021 tattaatgct gattatttaa atcttttaaa aagagcgtta aatatcaaat taacactccg
     1081 ggaatacgca gatcatcaaa aagcaatgga cgcgctggaa gacggtgaag ttgatatagt
     1141 gttatcacat ttagttgctt cgccgcctct taatgatgac attgctgcaa ccaatcctct
     1201 gataattacc tttccggcgc tggtcactac ccttcacgat tcaatgcgac cgcttacctc
     1261 atcaaaacca gtaaatattg ctcgagtagc aaattatccc ccagacgagg taattcatca
     1321 atcatttcca aaagcaacaa ttatctcttt tacaaattta tatcaggcat tagcatccg
//
LOCUS       DAZKJR010000363         1352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKJR010000363 DAZKJR010000000
VERSION     DAZKJR010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.366"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..715)
                     /locus_tag="ACHOWS_005355"
     CDS             complement(<1..715)
                     /locus_tag="ACHOWS_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0050761.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVKIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFY"
     gene            complement(741..>1352)
                     /gene="rfaY"
                     /locus_tag="ACHOWS_005356"
     CDS             complement(741..>1352)
                     /gene="rfaY"
                     /locus_tag="ACHOWS_005356"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFQ0050762.1"
                     /translation="ILAYNFKTVKVLRNIDDTKVSLIDTKYGRYVFKVFAPKTKRNER
                     FFKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVE
                     LNDMPIIPENVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAER
                     KARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          434 a          276 c          233 g          409 t
ORIGIN      
        1 cataaaagca atcataatcg ccgctaataa aaatacaatg accaaagaac agaatattta
       61 aaatatcctg atcaaggcat ttatatttcg tgtgctgcgg gtcacttgcc agcatagcaa
      121 tggctttatt cataaaatca tttttcgccc atgcttcaag ctgcagatat actacaccgg
      181 cgttgaaata attacctgga agtccctcta tagccagacg cttcgctggt ttttcctgca
      241 taaaaggcac atctttaata acagctgcga acttatcgcc atcaaaatta atatcaagca
      301 atccagctaa tgagccttta cagatgacat cagcatcgat atataacaga ttttcaataa
      361 cttcactcag atactggaac gcaactaaac ggaagaatgt tgcataagac cactgtttca
      421 ggctggggaa gatatcaata aaggcactgg agactttata aatttttact tcaacgttag
      481 catttttgct ggtaaaagcg ttaacacgtt gtagataatc ttcatcaatg tagtcggtaa
      541 agagatgaaa cttaaccgca aggtccgggt tatgcataac aactgattgc atagatactg
      601 ccgcaccaaa taaaaaatta cggtcaatac cgtaagcgat atttaacaca cgatcggtgt
      661 tagcaatttc cccagagaca gcgacttcag taatactctc agctaataaa tccatgttgg
      721 ttccgttttg actgtgtggt ttacgctttg ccttttaatt ttttaataaa tttgcgtagt
      781 ttggttctgt agattactga atagtagcca taatctttta tttcgttggc aatacccagg
      841 tgacgttcca tggccagacg atcacgagct ttacgctctg ccgtacagct tttaccggat
      901 aggtcaatta tcctgacacc atctttacta acgataaagt taccccggtg agggtcgccc
      961 gatagcatat ttaaggcatg caatttttcc attgaagcct taatttctgc tttaacattt
     1021 tctggaataa tcggcatatc attgagttct acgccctcga catactcgat aagcatgata
     1081 aaaacactgg catagttgaa aattttacgc tcagccagaa aataaaaatc attaggaaaa
     1141 gtaagacctg cacttctgac acgatcggtt tcaacgatca aattctgata gtagtcacct
     1201 tttacaaaag atttaaaaaa acgttcattc cttttcgttt tgggagcaaa gactttaaaa
     1261 acatagcgac catatttcgt atcaatcaac gatactttgg tatcatcaat attacgtaat
     1321 acttttactg ttttaaaatt ataggccagt at
//
LOCUS       DAZKJR010000364         1350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKJR010000364 DAZKJR010000000
VERSION     DAZKJR010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.367"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1350
                     /locus_tag="ACHOWS_005357"
     CDS             <1..>1350
                     /locus_tag="ACHOWS_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFQ0050763.1"
                     /translation="NRLPDPELHPDSTLTVWPDNRIAEDAHYVYRHDEYGRLTEKTDR
                     IPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRTGKRVWRRE
                     RDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDSTRIQTVYQPGSFTPLIRIETDVTELE
                     KAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRTDRVSSESRAWLAQCGLT
                     VEQLEKQVEPEYTPARKVHLYHCDHRGLPLALISEDGNTVWSAEYDEWGNLLNEENPH
                     HVYQSYRLPGQQHDEESGLYYNRNRYYNPLLGRYITQDPIGLAGGWSLYAYPLNPVNG
                     IDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKRLNLGGTDQFFHCMAFCRVSK
                     LNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSHSEMIEDNKKDLAVNEHGLTC
                     "
BASE COUNT          352 a          309 c          434 g          255 t
ORIGIN      
        1 aaccgcctgc cggacccgga gctgcacccg gacagtacac tcacagtgtg gccggataac
       61 cgcatcgcgg aggatgcgca ctatgtctac cgccacgatg aatacggcag gctgacggag
      121 aagacggacc gcatcccggc gggtgtgata cggacggacg acgagcggac ccaccactac
      181 cactacgaca gccagcaccg tctggtgttc cacacgcgga tacagcatgg cgagccactg
      241 gtcgagagcc gctacctcta cgacccgctg ggccgccgga cgggaaaacg ggtatggcgg
      301 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggaggt gacgtggtac
      361 gggtgggacg gcgacaggct gacgacggtg cagactgaca gcacgcgtat ccagacggtg
      421 taccagccgg gaagcttcac accgctcatc cgcatcgaaa cagacgtgac cgaactggag
      481 aaagcgcagc ggcggagcct ggcggagaag ctccagcagg aagggagcga ggacggacac
      541 ggcgtggtgt tcccggctga actggtgcgg ctgctggaca ggctggagga agaaatccgg
      601 acagaccgcg tgagcagtga aagccgggcg tggcttgcgc agtgcgggct gacggtggag
      661 caactggaaa aacaggtgga gccggaatat acaccggcgc gaaaagttca tctttaccac
      721 tgcgaccacc ggggcctgcc gctggcgctt atcagcgaag acggcaatac ggtgtggagc
      781 gcggagtatg atgaatgggg aaacctgctt aatgaggaga acccgcatca tgtgtatcag
      841 tcataccgtc tgccggggca gcagcatgat gaggagtcgg ggctgtacta caaccggaac
      901 cggtactaca atccgttgct gggacgatat attactcagg acccgattgg gctggcgggg
      961 ggatggagtc tgtatgcgta tccgctgaat ccggtgaatg gtattgatcc attaggatta
     1021 agtcctgcag atgtagcgtt aatgcgaaaa aaagaacaat taaaccatca aagagcatgg
     1081 gatatattat ctgatactta tgatgatatg aagagattaa atttaggtgg gactgatcaa
     1141 tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg tgttagccga
     1201 tcagcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa tatgttcggt
     1261 atgtacggca gaaaagtaaa gttatcccat tctgagatga ttgaagataa taaaaaagac
     1321 ttggctgtaa atgaacatgg gttgacatgt
//
LOCUS       DAZKJR010000365         1338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKJR010000365 DAZKJR010000000
VERSION     DAZKJR010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.368"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..110)
                     /locus_tag="ACHOWS_005358"
     CDS             complement(<1..110)
                     /locus_tag="ACHOWS_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFQ0050764.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWS"
     gene            380..577
                     /locus_tag="ACHOWS_005359"
     CDS             380..577
                     /locus_tag="ACHOWS_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050765.1"
                     /translation="MILSRKKIEIEVVQSGTADNPETVTKIKVSRPEDLEACGVIVEK
                     ICAIHAKSVNTTASNDHNKYN"
     gene            complement(694..834)
                     /locus_tag="ACHOWS_005360"
     CDS             complement(694..834)
                     /locus_tag="ACHOWS_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFQ0050766.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSQFAAFLQC
                     KQ"
BASE COUNT          339 a          323 c          311 g          365 t
ORIGIN      
        1 tctgaccaca tgatgttaag tctgaactca cacggagaac ggtgtatcat ccagacggtc
       61 aacaacatgg ttaagcataa aatgctcacg atgatcgttt tttgcggcat agcgcttgac
      121 tccttttaca gggaggcgct aaccttccac ttgtcacggt aggaagtgtt agggcctcgg
      181 gttaacattt aagttgactc ggggcctttc cacatccggc cttcaggtaa tccttccagc
      241 catcagccga aaggcacccg cgtgtaatgt actgatttcc ctttgtgtgc gcaatgcctg
      301 gcgttgaagc agacaacgct atcgcactgg tgatattctt tcgcacaggc ttactctgtc
      361 ctgtgtcggg gcgatgaaaa tgattctttc aagaaaaaaa attgaaattg aggttgtgca
      421 aagtggaacg gctgataatc ctgagacggt tacaaaaata aaggtttcac gcccggaaga
      481 tcttgaagcg tgtggtgtta tcgtggaaaa aatttgtgct attcatgcaa aatcagttaa
      541 taccactgcc agtaacgacc acaataaata caactgaaca aaaaagcccg catattgcgg
      601 gcaaatacgt ctgtaatcca atggagaaag gggtgccata tcgaccgaac ccggcaaccg
      661 gatagcgggg gctttcaccc ccgctgcggt ttcttactgt ttacactgca agaacgccgc
      721 aaattgtgac cacatgatgt taagcctgaa ctcacacggt gacttgtgta tcatccagac
      781 aatcatcaaa atagttatgc ataaaatgct cactatgatc gttttttgcg gcatagcgct
      841 tgactccttt tacagggagg cgctaacctt ccacttgcga ggtagatggg ttagggcctc
      901 gggttaaaca aatgtttgac tcggggcctt tccacatccg gccttcaggt aatccttcca
      961 gccgtcagcc gaaaggcacc cgcgcgtaat ttacctttta cgtccgttac gtgcaacgga
     1021 tgacgtggct gtttttcctt ccccttcgcc atcctttttc ggagcagttg tgcaaaagtg
     1081 tgcaaaagtg atttttgttg ccactccccg ccaggccgcg ccagttctgg ctttcctgat
     1141 tttttcgttt tcgtcgccgg atcctttttc agatcttcga tcgtgacgta attttaaaaa
     1201 tctcttgtta ttcagtgcat taaaattttt cgggatcctc agaaagccgg agtttttgca
     1261 cacttttgca aaaccttgca caaagtgcaa aaccattcag aagggaaaac ctcagtaatg
     1321 gcgcgccctg gcggggtg
//
LOCUS       DAZKJR010000366         1329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKJR010000366 DAZKJR010000000
VERSION     DAZKJR010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.369"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..407)
                     /locus_tag="ACHOWS_005361"
     CDS             complement(144..407)
                     /locus_tag="ACHOWS_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0050767.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            597..839
                     /locus_tag="ACHOWS_005362"
     CDS             597..839
                     /locus_tag="ACHOWS_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050768.1"
                     /translation="MNNESEKAMWRCKPVDNPWTLDSLLKKTISAVLPASDKVSHQNK
                     NKLRNNEFIKIEHPCSKSESRIDGDYIISGGTEYRL"
     gene            complement(887..>1329)
                     /locus_tag="ACHOWS_005363"
     CDS             complement(887..>1329)
                     /locus_tag="ACHOWS_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0050769.1"
                     /translation="AMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLITLTFVVFA
                     VHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGTMFLVLPA
                     VWLGALSWAGVHIGDGISRGLPNGISEAKGAASSAGSIANRGIK"
BASE COUNT          391 a          327 c          261 g          350 t
ORIGIN      
        1 tggcatccac atagtgcgct gggttatcgc tctccacggg aatatctgcg gcggcgggca
       61 gtaatgggtt aagtgatacc agatgtctgg aaatatagga gcaaatccac cacgcatgga
      121 gcctgttaag actctcactc tcatcattct cccttctgag tcccaacgaa acgaccaaaa
      181 cgctgagtaa agcgggcaac attgccttct gatgccaccg ttctgagttt accggtatag
      241 aacggatgcg atttggagga aacgtcaatc gtcacatacg gatacgtcac accatccagt
      301 tcaatctcgc gatctgtttt gatggtagaa cctattttaa agtactcatc cacactcgtg
      361 tcatggaaca ctactgtgcg gtattcagga tggatatttg gtttcatctc tcttcactca
      421 tgttataaca taacaataag atacattctc agctagatca tttcaacaga atccccgagc
      481 ccaagggtaa tgtggcatcg cagcctctca tcaaatggaa gacatgtgat tgcacattta
      541 tcatacaaga cgtatgataa cactgtttta tttatcctgc tgccgagggt atagatatga
      601 ataacgaatc tgaaaaagca atgtggcgtt gtaagcctgt ggataatcca tggacgttgg
      661 attcattact aaaaaagact atttctgctg ttttacctgc atcagacaaa gtttcacacc
      721 aaaataaaaa caaattacgt aataatgaat tcattaaaat tgagcatcca tgctcaaagt
      781 ctgaatccag aatcgatggt gactacatca ttagtggagg aacagaatac cgtttgtaat
      841 taagcaggag aattttctgt aaattctcct gtaacttttt tacttcctat tttatacctc
      901 gattagcaat acttcctgca ctcgaagctg cacctttcgc ttccgaaata ccattcggta
      961 atcctcgact gattccatcg ccaatatgaa caccagccca ggacaacgct cccagccaca
     1021 ctgccggcaa gacgagaaac atcgtcccca tcaccagatt cattatcaga tcatccgatg
     1081 tgttctggaa tcccatcata ttgaaccggg tatgtgtatc cgaactgtac agcgccgtca
     1141 gcatccagct gtcaagccac cgtgcaagct cccaccagaa cgtcagaaag tgtaccgcaa
     1201 acaccacaaa cgtcagtgtg attaacgtcg acagttcata tgccgcaaac gccagaatca
     1261 gcggtatcat gatgtaaatc gccatcagta ttatcgactg taccatcggc agtgcctgcc
     1321 gcattgcat
//
LOCUS       DAZKJR010000367         1315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKJR010000367 DAZKJR010000000
VERSION     DAZKJR010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.370"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..1229
                     /locus_tag="ACHOWS_005364"
     CDS             147..1229
                     /locus_tag="ACHOWS_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFQ0050770.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          397 a          273 c          284 g          361 t
ORIGIN      
        1 aaaaagttca gataaaaata gagatctact tcacaaatca aacgagaaac caaaacttac
       61 atcttgaaat aatcacattg attagatgaa tatttatcgc gcagtgacat cattttttaa
      121 taatagttca aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      181 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      241 acaagctgga tctatatggg aaagttaatg ccaagcacta cttctcctct aatgatgcag
      301 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      361 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      421 gctcctccaa agacaaaacc cgtcttgcat ttgcaggcct gaaattcggg gactacggct
      481 caatcgatta cggccgtaac tacggtgtag catatgacat cggtgcgtgg actgacgttc
      541 tgccagaatt cggtggcgat acctggaccc aaacagatgt gttcatgact ggtcgcacca
      601 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      661 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      721 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
      781 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
      841 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
      901 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
      961 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1021 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1081 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1141 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1201 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1261 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaa
//
LOCUS       DAZKJR010000368         1312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKJR010000368 DAZKJR010000000
VERSION     DAZKJR010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.371"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..834
                     /locus_tag="ACHOWS_005365"
     CDS             <1..834
                     /locus_tag="ACHOWS_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFQ0050771.1"
                     /translation="PRQVWRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFAT
                     YITRQGGVLIPRNTYLVRTAYETAEEANDYGEFPQRIYGVRAPSLGERYTICTHPDTW
                     KLVRRKPENEDRTDEGFDVECGFSAPWTRGNNCPPDQNLTINDPPERQPDGRREQRLT
                     LPEGDLGEWLRSLTPTERKQLTRQLKSPPPDTETVKNGGISAPAGGVRVSVALTAEPH
                     ITAQITRELDALGVQLHESAVVSVRHGARVRLADGRTSYWNDSTRRIDITPAPNTEAP
                     Q"
     gene            831..1235
                     /locus_tag="ACHOWS_005366"
     CDS             831..1235
                     /locus_tag="ACHOWS_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050772.1"
                     /translation="MTRFYCAMQRQRFDELMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTTIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLASINRKAAIARYVHSAAGRQSRR"
BASE COUNT          322 a          444 c          352 g          194 t
ORIGIN      
        1 ccccgccagg tatggcgcga actccgccgt cttgccgggc aaatggcccg taacccggct
       61 gccccgcagc gtctggacca tgacgacatt gacgccatac tggccgccgc cgacgtggga
      121 tgttttgcca cctatatcac ccgccagggt ggtgtgctca ttccccgcaa cacgtacctg
      181 gtccgcaccg catacgaaac tgccgaagag gccaacgact acggcgaatt cccgcaacgc
      241 atttacggcg tccgcgcccc gtcactcggg gagcgctaca ccatatgcac ccacccggac
      301 acctggaaac tggtcagacg taagccggaa aacgaagacc gcacggacga gggttttgac
      361 gttgagtgcg gcttttccgc accttggact cgtggcaata actgtccccc tgaccagaat
      421 ctgaccataa acgacccgcc agaaaggcag ccggacggac gcagggagca gcgacttacc
      481 ctgccggaag gtgatttggg tgaatggtta aggtcactga cccccacaga gcgaaaacaa
      541 ctcaccaggc agctgaaaag cccgccacct gacacggaaa ccgtgaaaaa cggcggcatc
      601 agtgcgcccg ctggcggcgt tcgcgtgagt gtcgctctga ccgctgagcc acacatcacc
      661 gcacaaatca cccgcgagct tgatgcgctg ggcgtgcagc tgcatgaaag cgccgtcgtt
      721 tccgtccgtc acggtgcccg cgtgcgcctg gctgacgggc gcacctcgta ctggaatgac
      781 agcacccgcc gcattgatat cacccccgca ccgaatacgg aggcgccaca atgacccgct
      841 tttattgcgc catgcagcga cagcgttttg acgaactgat gcgccagcgc acgcccttac
      901 tgaccctgaa caacattgcc acggggccac aaccgggcga tgagctggaa atcacggtcg
      961 tcagtcccga tcgcaccacc acgaccatca tcaccgacat actcaacgga cgaccggaca
     1021 gcaccaaccg cagctggctt gtcgccgttc gtccgcgccg tccgcccgga gagccggagc
     1081 cgctcacgcc tgccggaaaa atcatgcggc agtaccacga agagcaggca cacaaacaca
     1141 acgcagaact caccagaaca ctggcatcga ttaaccgcaa agcggcaatt gcccgctatg
     1201 tgcattcagc cgctggcaga caatcacgca ggtaaacaaa gaccatgaac ataaccagaa
     1261 acagccaact aaaggagcgc accatgaaca gcaccaggag gatcagcgac ag
//
LOCUS       DAZKJR010000369         1311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKJR010000369 DAZKJR010000000
VERSION     DAZKJR010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.372"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            662..>1311
                     /locus_tag="ACHOWS_005367"
     CDS             662..>1311
                     /locus_tag="ACHOWS_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050773.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPIFFSHGSHIHSYFICSPFFLVHEEIKNN
                     NPELFPHGFDIIIEDTTFQMYSPDREQQIKHVKKTLKYDGIFIFTEKFSTSTKEYQ"
BASE COUNT          450 a          267 c          228 g          366 t
ORIGIN      
        1 gtaagaacat tccggcatca ccggatagat aaggatcaca caaccactgc tgtgtattgc
       61 ccgtaataaa gggcgcggtt aaaagaaacc ttcatcatca tgactgacga tatctgctct
      121 caaaggtggg cagatttacc accaccagaa acatcctagc tgcacatccc ctttcaggtt
      181 gcgggggtgt tgatcacccg aaggcgttcc tctcagatca acggaacatt ggtgcccacc
      241 gaagcgggat gtggtcaccc gaaggcgctc ctctcagacc acttatggag cattggtggt
      301 cgccgaagcg acagataatg cattctgagc atatcagagc gcataaaagg cgtcaattat
      361 ccatgctccc tcacgaaaca gaagctgaac tcttaacacc tgctatcgga actcactgaa
      421 taaataacta aaaaggacat tgtgaaaatt aacatcaaca gtatgtaaag tgatttgttt
      481 tgtcttaaat gggtgaatgt aaaaaaatat tctatacaat ccacacatcc ttgtatcata
      541 tatcaagtaa acatccacag agataacagc ttatttcaaa taagcagatt ctgtttgata
      601 cttcagaaaa atagtatatg ctcctgattt actgatgtta taagttatac aggatagtta
      661 aatgtgtgat tttataaaat catgttatgc agcaccaaga attaacaaaa tggctgattt
      721 tttctcatca acagcatctg gagtaaaaca taaacctttg aagcatgtaa aaattgataa
      781 caggcttttc ccatctcctg agtatataat tttttctgac tatcttacga aaaacagtgg
      841 tccatttgtt aaccactact tttgcagtat tccatacagc aaagaagaag aatgcaggct
      901 gggtgtgact attatgaaat atgcaagcca tcgaaataag ccattgagct tatggtgtct
      961 tggaatggca gaaggagcca tggccagaac aatttctcaa ctatcaaaag gacaggtttt
     1021 atcattaaca accagcccaa caaaagaaaa tgaacctata ttctttagtc atggttctca
     1081 tattcactct tactttattt gctccccatt ctttcttgtt catgaagaaa taaaaaataa
     1141 caaccctgaa ctatttcctc atggttttga tattataata gaagatacaa cgtttcagat
     1201 gtattctccc gacagagaac aacaaataaa acatgtaaaa aaaacattaa aatacgatgg
     1261 tattttcatt ttcacagaga aattttccac ctccacaaaa gaatatcaga t
//
LOCUS       DAZKJR010000370         1304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKJR010000370 DAZKJR010000000
VERSION     DAZKJR010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.373"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(38..421)
                     /locus_tag="ACHOWS_005368"
     CDS             complement(38..421)
                     /locus_tag="ACHOWS_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFQ0050774.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            complement(792..>1304)
                     /locus_tag="ACHOWS_005369"
     CDS             complement(792..>1304)
                     /locus_tag="ACHOWS_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050775.1"
                     /translation="RFSKLLPFRIDFAYRKDTPSFGHSCKHSMCMEIYRLLSETQTML
                     AGYYWVMEYTPDKGLHIHFIGYLDGQRHKNSYQISRQLGDIWRRITEGEGYFHLCRAK
                     DKYPVRIDHVIHYSDKSAVDDLRYALSYLAKQDQKEHGIILRRSRLPEKSNRGRPRLD
                     STLSEICSLV"
BASE COUNT          303 a          334 c          338 g          329 t
ORIGIN      
        1 tcacccggcg tgatccccag ccccgttttt ataccgttca ttctgctccc ccctcctcac
       61 tgaagcgccc ggtctggcag tgtgtgcagt actgctcccc ataataatcg tggtgacatt
      121 gcctgcagtg ccagctggct ttacgtacta cgggtaaggc atccgggacg aattgctgca
      181 gacgtttaat cagttgtatc tccctgcgtt ccggtctgac ataagggcac tgctgaccgt
      241 gctccgtcag cccgtcgtca gtgtgttcaa accagggacg ctcagtgtcg tattgcgggt
      301 ggtatctgag cgcactgccg caaaggtggc aagtgtagcg atcgtaaggt gcagtctgtg
      361 cggtacgggc acccgtcaga cgcccgttgc catcaaatgc aataaaggat tttgcgtaca
      421 tcgtcatatt cctcagccgc cagacgacac gcaggcaaca gctgtcccgg tacgggcaga
      481 gtggtcaggg tgtgaatggc ggttcagtta aaatctggac tggagtttgg gtaaaacagg
      541 ctggttgctg ttaccgcact gtggttgtca cacaatggcc cacctgcgta aaaaactgtc
      601 agcaggcagg gatggacatc gggggatgtg ctgtatcggt tgccggaagg aatggtaaag
      661 aatgtgccat ggttaaatct ccgtatggtt catacggaat agccacatcg taaaaatgcg
      721 cagtgtcgct gaaatggcct ccggaaccga ctgtaacaat tagcgccagc ccgcagttgt
      781 cgttcagggg cttacaccag agaacatatc tccgacaggg tactgtccag tcgcggtcgg
      841 ccccggttac ttttttcggg aagcctgcta cgcctcagaa taataccgtg ttctttctga
      901 tcctgtttag ccaggtagct gagggcgtaa cgtaagtcgt ctaccgcaga tttatcacta
      961 tagtgaatca cgtgatcgat acgcaccggg tatttgtctt tagcccggca cagatgaaaa
     1021 taaccctccc cttccgtgat ccgcctccag atatccccca gttgcctgga tatctgataa
     1081 gagttcttgt gacgttgccc atccagatag ccgataaaat gaatatgaag ccctttgtct
     1141 ggtgtatatt ccattaccca gtaatatccc gccagcatcg tttgggtttc gctaagtaaa
     1201 cggtatattt ccatacacat actgtgctta caggaatgac caaaactggg cgtgtctttc
     1261 ctgtaggcaa aatcaattct gaaaggtaac agtttagaaa aacg
//
LOCUS       DAZKJR010000371         1269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKJR010000371 DAZKJR010000000
VERSION     DAZKJR010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.374"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1269
                     /locus_tag="ACHOWS_005370"
     CDS             <1..>1269
                     /locus_tag="ACHOWS_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFQ0050776.1"
                     /translation="SAIHDLTLDALNKNTAVLSNGIELKIGSSINPYSYDQTLADNMM
                     RKAVKEHFKLEKEYLTQRPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEAN
                     ELLKTEKDEFYRNYLEKTVKDISSVHGGYFSKDNSDKDDKIEQEINEILHDKELLLSL
                     ENPRRFIFSKWTLREGWDNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVKN
                     RDFTLNYYVDFTEKDFVDSLVKEVNDSSFKETVPGKFTQELKDKILSQYPELSSRTLM
                     NEIFDAEIIDDNENFKDSDAYSRLKAKYPAAFPAGVKPSKIKKATDGKKRTKMRIGKF
                     SELKELWDLINQKVVIEYKINSENEFLSIFKSFMIEETDRFTKSGVHTRIDRIYIHND
                     TAMSKSIVSDDDNFAKLNTMSYREFLDNLSQ"
BASE COUNT          445 a          201 c          246 g          377 t
ORIGIN      
        1 agtgcgatcc atgatttaac tcttgatgct ttaaataaga atacagcagt gttgagtaat
       61 ggtattgagc taaaaattgg tagctcgata aacccctatt cttacgatca gacacttgct
      121 gataacatga tgcgtaaagc tgtcaaagaa cactttaagt tagaaaaaga gtatctgaca
      181 caaaggccac gcataaaacc tcttactctt ttcttcattg atgacattga aggctatcgt
      241 gatggtaatg atattgcagg aagcctgaaa actaagttcg aagaatacgt tttggcagaa
      301 gcaaatgaac tgttaaaaac agagaaagat gagttttaca gaaactacct tgaaaaaact
      361 gttaaggata tatcttctgt tcatggtggt tatttttcaa aagataatag tgataaagat
      421 gataaaattg agcaggaaat caacgaaatc cttcatgata aagagcttct attgtcttta
      481 gaaaatcctc gccgtttcat tttctctaaa tggacgttgc gtgagggatg ggataaccca
      541 aatgtctttc agatttgtaa gctccgttcg agcggtagca ccacatctaa acttcaggag
      601 gtagggcgtg gtttacgtct tccagtgaat gaatacatgt gccgtgtgaa aaaccgcgat
      661 tttactctca actattatgt tgattttacg gaaaaagatt ttgttgattc tcttgtaaaa
      721 gaggttaatg atagttcttt caaagaaact gttccaggta agtttactca agaacttaag
      781 gataaaatct tatctcagta tcctgagctt tcatccagaa cactcatgaa tgagatcttt
      841 gatgctgaaa ttattgatga taatgaaaac ttcaaagatt ccgatgccta tagccgttta
      901 aaagcaaaat atccggctgc attccctgcc ggggtgaaac caagcaaaat aaaaaaagcc
      961 acggatggaa aaaagcgtac taaaatgcgt attggtaagt tcagtgagtt gaaagaacta
     1021 tgggatttga tcaatcaaaa agtagtaatt gagtataaaa taaatagtga aaatgaattc
     1081 ttatccattt tcaaatcatt catgattgaa gaaacagata gatttactaa atcaggtgtt
     1141 catactcgca ttgatagaat ttatattcat aatgatacgg cgatgtcgaa aagtatcgtc
     1201 agtgacgatg ataactttgc taaactaaac acaatgagct atcgggaatt tcttgataac
     1261 ttatcacaa
//
LOCUS       DAZKJR010000372         1266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKJR010000372 DAZKJR010000000
VERSION     DAZKJR010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.375"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            125..388
                     /gene="ybeD"
                     /locus_tag="ACHOWS_005371"
     CDS             125..388
                     /gene="ybeD"
                     /locus_tag="ACHOWS_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFQ0050777.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            489..1130
                     /gene="lipB"
                     /locus_tag="ACHOWS_005372"
     CDS             489..1130
                     /gene="lipB"
                     /locus_tag="ACHOWS_005372"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFQ0050778.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT          325 a          313 c          282 g          346 t
ORIGIN      
        1 cactggtttg gttaaaaatt gaacacttga aagtgtaatt tccgtcccca tatactaagc
       61 atcagtaaaa aactcccgcc ttctggcggg agttgctatt taattacgtt acgccggagc
      121 tgacatgaaa accaaactta acgaactgct tgaattccct actcctttta cttacaaagt
      181 tatggggcag gcgttacctg agctggttga tcaggtggtt gaagtggtac agcgccatgc
      241 gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa ggcaactacc actcggtatc
      301 tatcactatc aacgccactc atatcgagca ggttgaaaca ctgtatgaag aactgggcaa
      361 aatcgatatt gtccgcatgg ttctgtaact cgcttctccg ttacccggct cctggtcggg
      421 taactcccct ctcctcgctg tgatatactt ttcccccact tttactcatt ctccacggag
      481 atgccgtttt gtatcaggat aaaattcttg tccgccagct cggtcttcag ccttacgagc
      541 caatctccca ggctatgcat gaattcaccg atacccgcga tgatagtacc cttgatgaaa
      601 tctggctggt cgagcactat ccggtattca cccaagggca ggcaggaaag gcggagcaca
      661 ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg cggtgggcag gtgacttatc
      721 acgggccggg gcaacaggtg atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg
      781 tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa taccctggct gaactgggta
      841 tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct
      901 cactgggttt acgtattcga cgcggttgtt cattccacgg tctggcatta aacgtcaata
      961 tggatctttc accattttta cgtattaatc cttgtgggta tgcaggaatg gaaatggcta
     1021 aaatatcaca atggaaaccc gaagcgacga ctaataatat tgcaccacgt ttactggaaa
     1081 atattttagc gctactaaac aatcctgact tcgaatatat taccgcttaa ttccatacat
     1141 caatggccca atttacactg ggtcattact cataatacct gctaatgctt tacatctcct
     1201 tcgcgtttgt atattttctt tatcacttgc agaaatgcga tgctacgcct tgctccgtag
     1261 cccacc
//
LOCUS       DAZKJR010000373         1258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKJR010000373 DAZKJR010000000
VERSION     DAZKJR010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.376"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(260..547)
                     /locus_tag="ACHOWS_005373"
     CDS             complement(260..547)
                     /locus_tag="ACHOWS_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFQ0050779.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(622..765)
                     /gene="mgrB"
                     /locus_tag="ACHOWS_005374"
     CDS             complement(622..765)
                     /gene="mgrB"
                     /locus_tag="ACHOWS_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFQ0050780.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            924..1163
                     /locus_tag="ACHOWS_005375"
     CDS             924..1163
                     /locus_tag="ACHOWS_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFQ0050781.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
BASE COUNT          400 a          274 c          240 g          344 t
ORIGIN      
        1 gaatgcgcaa aaaaatcaag agagaaacgt ttcgctaata aataatacca cgcttaatca
       61 caaatccgat gtaaacatcc taataaatta atgggctaaa tatctttttc tgatatcccg
      121 ttacgaaaat aaataatatg ataattattt gaagtcagac cagagtttta atttgacgca
      181 gttgcaccat gacagtgcgt ataatcagga tgaattgagt aacccttcaa cgacggggtt
      241 actcaagatt gaggaaagat tatcgttcgg cgaccagacg aataatatct tcgtcttcaa
      301 ccgatttttg actctcaacg gtctttttat ctgctttctc tggttcgttt gcttcgcaca
      361 aacgacgtaa cagagcattt tgacgttttt gctgatccag caacgcctca agcagttcaa
      421 tctgttcgtt agtccgtgaa ctggcacgat tgataaaaaa ccacaagata agcccgataa
      481 aaagaaccac caccgataca accaaagacg caatgttcat cacgcctgaa tttacaactt
      541 cgttcatttc accacctctg agtagaggcg ctattctacc actgctggag aggaagaaaa
      601 tctagtgctg aaaaaatgat atcaccacgg gataaactgg ttaatggcac aaattccgct
      661 gaaaaattgt acatcctgat cgcacatcat gttgaatacc tgcgcccaaa gcagcaagca
      721 agccaacacc acgacaacca gaacgaccca tcgaaacttt ttcactccaa tctccgtttc
      781 acctacctta tgtcattaaa ctagcatgcg ttagttaaac agcgcctaac tatgtcgata
      841 ttcgtgcttt ttcagaatga gtcgcttgct tcagcgcata ttgccgctac gattaagcga
      901 acataaaaag agaagaggtt gtaatgcgat tcatcattcg cacagttatg ctgatagcac
      961 tggtatggat aggattatta ctcagcggat atggcgtttt gattggaagc aaagagaatg
     1021 ccgcgggatt aggtttgcaa tgtacctatc tgaccgccag aggcaccagc acggtacaat
     1081 acctgcacac taaaagtggt tttctgggga taacagattg cccgttgtta cgtaaaagca
     1141 atattgtggt cgataatggt taagggtaat aaataaccag attctcactc atgagtaaaa
     1201 tattcttatc gcaagtgcgt ttaatataac gccaccttca actttatttt tctcatgc
//
LOCUS       DAZKJR010000374         1257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKJR010000374 DAZKJR010000000
VERSION     DAZKJR010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.377"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..276
                     /locus_tag="ACHOWS_005376"
     CDS             <1..276
                     /locus_tag="ACHOWS_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0050782.1"
                     /translation="TTLEIAKTVNIENSEFLKAVALMGGGIAYLPYPLVQSEILSGKL
                     VSIMNDYIKTRFEVSLWFRKPLSLRCVTFRNFLVERASQLKDTVQFR"
     gene            450..1257
                     /locus_tag="ACHOWS_005377"
                     /pseudo
     CDS             450..1257
                     /locus_tag="ACHOWS_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          378 a          275 c          305 g          299 t
ORIGIN      
        1 acaacattgg aaatagctaa aacagtaaac attgaaaata gtgagttttt aaaagcagtt
       61 gcactaatgg gtggggggat agcatactta ccatatcctc ttgttcaatc agaaatactg
      121 tcaggaaaac tagtatcaat aatgaatgat tatattaaaa ctcgttttga agtaagtctg
      181 tggtttagga agcccctatc cttgcgttgt gttacattta ggaatttcct agtagaaaga
      241 gcctcacaac tcaaggatac tgtacaattc agataaatca cttaacttga tgcatcaatc
      301 acccagcgac cagttatgct catcataatc attattcagg tgtgagcata atgagcataa
      361 cccagcgatc cagacaggtt ccctgaatct ccagacaacc aatatcaatt aaataagtga
      421 tagtctaaaa actagtcatt agagaacaga tgattgatgt cttagggccg gagaaacgcg
      481 gacggcgtac cacacaggaa aagatcgcta ttgttcagca gaactttgaa ccgggaataa
      541 ctgtctccct cgttgcacgg caacacggtg tgacagccag ttatttctct ggcgtaagca
      601 ataccaggaa ggaaatctca ctgctgtggc tgctggagaa caggttgttc tggcctctga
      661 gcttgctgcc gccatgaagc acattaaaga actccagcgc ctgcccggca agaaaacgat
      721 ggaaaattaa ctcattagag aagccgttga atattggcgt gcaaaaaagt ggatagcgca
      781 cgcccaatat tgcccgggaa tggggagtaa gtttcgtcag ccgttatctc cgtgtgttgc
      841 gtgcgcagtt gcacgtcatt ctcagacgaa ccgatgactg gaaggatggc cgccgcatga
      901 tacggatgtt tatctccgta ataacatggt gtacaccatg ttatcggaga gctgcccacg
      961 tatggttatc gtcggctgtg aaacagtaca ggacgtcatg ctgggagcgg tagaacaccg
     1021 cttcggcagc gaccttcctg cgtctccagt ggagtgcctg acggataata gttcatgcta
     1081 tcaggctaat gaaacacgcc agttcgcccg gatgttggaa cttgaaccga agaacacggc
     1141 ggtgcggtat ccggagagta acggcatagc agagagcttc gtgaaaacga taaagcgtga
     1201 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcaga
//
LOCUS       DAZKJR010000375         1242 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKJR010000375 DAZKJR010000000
VERSION     DAZKJR010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.378"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1242
                     /gene="tssC"
                     /locus_tag="ACHOWS_005378"
     CDS             <1..>1242
                     /gene="tssC"
                     /locus_tag="ACHOWS_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFQ0050783.1"
                     /translation="LIDHHIAELDDQISRQLDAVLHHPEFQKVESLWRGVKSLVDKTD
                     FRRNVKIELLDLSKDDLRQDFEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALVSAWEF
                     DASAQDVALLKNISRVAASAHMPFIGSVGPAFFRKETMEEVAAIKDIGNHFERAEYIK
                     WNAFRETDDARYIGLVMPRVLGRLPYGPDTVPVRSFNYVEEVKGPDHHKYLWTNASFA
                     FAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIPSEVMIPETREFE
                     FANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINARLPYIFLLSRVAH
                     YLKMIQRENIGTTKDRRLLELELNTWLRGLVTEMTDPGDELQASHPLRDGKVVVEDIE
                     DNPGFFRVKLYAIPHFQVEGM"
BASE COUNT          270 a          293 c          358 g          321 t
ORIGIN      
        1 gctgattgac catcatattg cagagcttga tgaccagatc agtcgtcagc tggatgccgt
       61 gcttcatcat ccggaatttc agaaagtgga atccctgtgg cggggcgtga agtctctggt
      121 ggataaaacg gacttccgcc gtaatgtgaa aattgagctg ctggatctgt ccaaagacga
      181 tctgcgccag gattttgagg atgcgccgga aattattcag agcggccttt atctgcagac
      241 ctatgtggct gaatatgaca cgccgggtgg tgaacccatt gctgccctgg tttcggcctg
      301 ggaatttgat gcatctgcgc aggatgtggc gttgctgaaa aatatttccc gggtggcagc
      361 atccgcacat atgccgttta ttggttctgt tggtccggct ttcttccgga aagaaaccat
      421 ggaagaggtg gctgctatta aggatattgg caatcacttt gagcgtgccg agtacatcaa
      481 gtggaacgct ttccgagaga cggatgatgc ccgctatatt ggtctggtga tgccccgtgt
      541 gctgggacgc ctgccgtatg gccctgatac tgtacctgtc cgcagcttta actatgttga
      601 agaggtgaaa gggccggatc atcacaagta tctctggacg aacgcgtctt ttgcttttgc
      661 ggccaatatg gtgcgcagtt ttgtcaccaa cggctggtgt gttcagatcc gcggtcctca
      721 ggccggtggt gcggtgcagg atttgccgat ccatctgtat gatctgggaa ccggtaatca
      781 ggtcaaaatt ccgtcagagg tgatgatccc ggaaacgcgg gagtttgaat tcgctaatct
      841 gggttttatt ccgctctctt actacaagaa ccgggattat gcctgtttct tctccgctaa
      901 ctccacccag aaaccggcac tgtacgatac gccggatgca acggcgaaca gccgtattaa
      961 tgcccgtctt ccctatattt tcctgctgtc ccgcgttgcg cattacctga agatgattca
     1021 gcgggaaaat atcggtacca ccaaagatcg gcgtctgctg gagctggagc tgaatacctg
     1081 gctccggggc ctggtgacgg agatgaccga tccgggcgat gagcttcagg catcgcatcc
     1141 gctgcgtgac ggaaaagtgg tggtggaaga tattgaagat aacccggggt tcttccgtgt
     1201 gaaattgtat gccattccgc atttccaggt ggaaggaatg ga
//
LOCUS       DAZKJR010000376         1232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKJR010000376 DAZKJR010000000
VERSION     DAZKJR010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.379"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(242..1067)
                     /locus_tag="ACHOWS_005379"
                     /pseudo
     CDS             complement(242..1067)
                     /locus_tag="ACHOWS_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          313 a          299 c          271 g          349 t
ORIGIN      
        1 caggcaatta tcacaggcaa aaaaacaata tcttgtaagc ttcatagaat ttggaacttt
       61 atataataaa taatgctgag ttttttatca ccaatacgtt aacaaaatta atgtattcca
      121 gagatttcac attagtctcc aatttttcag aagaatctat ttacccggca ggaaaagtca
      181 tctggtgttt cgccggagtg aggggtacag gcaatagcgg acataaccat ttgttctttt
      241 tctaagcagc catctgataa tattttatcc tgaaggctgt cggggagata ttccccagac
      301 gagagtgacg acgctgacga ttgtagaaaa tctcaatgta ttcccgtatt actgagatgg
      361 cctcatcccg gcttttaaaa cgatagtggc tcagactttc atttttcagc gttccccaga
      421 agctttccat cggagcattg tcgtaacagt taactttacg cgacattgat attttcagac
      481 caaactgctc ctgtatggcc ctgtaatcgt atgcgcagta ctgtgaacca cgatcggagt
      541 ggtggattag cccggtaggt gagcgctggt tcctgagcgc cataaacagg gctttacctg
      601 tcagctcttt tgtcatgcgc tctcccatgg cgtagccgac aatttcgcac gtataaacat
      661 ctttgacgcc agcgatatac agccatcctt ccggggggca acatacgtta tatctgcaac
      721 caagacctga ttaggtgctg taggcgtaaa gttctgattc aggagattcg gcgcaaccgg
      781 cagattgtga ccagagttcg tgttcgccct gaacttgcgt ttcagcttac agtgcagcct
      841 cagctcctta cgaagacgtg ccagtctgtc acgaccgacg ataattccgt tatctgccag
      901 ctccgtctgg agcagctagg ttccatatgt ttcgcgtgtg cggatatgtg ccaccttaat
      961 ctccagtttt agccgctcat cactttgttt tctgtctgag ggtcatgctg tactcagtcg
     1021 taataaccga tcctgtatac accaagtacc cgacacatcg cttcaataca taatcgcatc
     1081 tgaatcgttc accgattcgc ggcatttttc aaggtagttg tcgccattgt tcgactaact
     1141 gatcgacatt acagcctaga gcctagctgg gggctgtgca caaaaaagcc ccggacattc
     1201 cggggctaca ctgcatatta acgaacgctc tg
//
LOCUS       DAZKJR010000377         1222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKJR010000377 DAZKJR010000000
VERSION     DAZKJR010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.380"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..542)
                     /locus_tag="ACHOWS_005380"
     CDS             complement(<1..542)
                     /locus_tag="ACHOWS_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFQ0050784.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGD"
     gene            complement(542..871)
                     /locus_tag="ACHOWS_005381"
     CDS             complement(542..871)
                     /locus_tag="ACHOWS_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0050785.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRTAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(868..>1222)
                     /locus_tag="ACHOWS_005382"
     CDS             complement(868..>1222)
                     /locus_tag="ACHOWS_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0050786.1"
                     /translation="GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAE
                     GGYVGGAGSPAQMRRVEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDEL
                     RLQLRDGGMLSGSGR"
BASE COUNT          252 a          462 c          261 g          247 t
ORIGIN      
        1 tcatcgcccc ggtaatccca catacaggtg ttcgccagca tgatgcgacc gggaaacagc
       61 gccccgtccg tctccgtcgg tgtcgccagc acaaacgagg ccgtcatggc cgtcagcgat
      121 gacatctgct ccaccaccca ccggtccgtc agctcctgct ccgggtcggc ctcaggattg
      181 cctgccacaa aattcaccgc atccagaaaa cgcgcataca cccggcgacg gaccaccgtg
      241 gcacccacca ggctctgcaa atcctccgcc atcccggtga caaggccgaa aagattggag
      301 accgtcagcg acgggcgggc actgctgccc tttccgttca tctcaaagcc gctgccctca
      361 atcgggtacg cctgatattc acgcccctgc caggtcaccg gctccccttt ttcattcagc
      421 tcattgcaga aaaaataccg ctcaccgccc tgcaccgtca ggtcgatttc ccagagcacc
      481 acccgcggtg actgctctga cttaaccgac tcgttcaggc ttgcttcgtg aatatcctgc
      541 atcagttcac cacctgctta aactccgcgc tgaactcaac acgcaacatc ccgacccgcg
      601 cagaccaccc ggcacaggtc acctttatct gccggtatgc atagggtggc ttccacaaaa
      661 atgccttcca gcccccgtgc tctgccagaa acgcttccag atgccgggcc tcctcccggg
      721 tcacggaaag catcaccctg tacgtcttca ggtcagcatt cagccctgcc gccgttcgct
      781 gcgaataccc gtcaccaaaa cgcacttcac gcaccgacgg ctgcgagttc acctccatat
      841 ccggcttcac tttccagcga aaggttttca tcgcccgctc cctgataaca taccgccatc
      901 acgcaactgc agccggagtt catcctgcgc ccccttgcgg gccatgtcat acaccgcctt
      961 catcagctgc ggccccgcct gtccgtttat accgtcgttc tgaatcacca cgtgattgtt
     1021 ctgattaaaa ttaatacctt ccacccgccg catctgcgcc ggacttccgg ccccacccac
     1081 ataaccacct tccgcatagc cgcgcatcag acggtaaaga ttccccacac ctatccggct
     1141 ggttgcctct ttcgtgaaaa caaactcccc gcggtgaact atccccgcag gctcatattt
     1201 gccgcccgtc cccgtaaatc ct
//
LOCUS       DAZKJR010000378         1220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKJR010000378 DAZKJR010000000
VERSION     DAZKJR010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.381"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1220
                     /locus_tag="ACHOWS_005383"
     rRNA            <1..>1220
                     /locus_tag="ACHOWS_005383"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          339 a          258 c          383 g          240 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 aagtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1021 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
     1081 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatat
     1141 gtaggtgaag cgacttgctc gtggagctga aatcagtcga agataccagc tggctgcaac
     1201 tgtttattaa aaacacagca
//
LOCUS       DAZKJR010000379         1190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKJR010000379 DAZKJR010000000
VERSION     DAZKJR010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.382"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1190)
                     /gene="tssF"
                     /locus_tag="ACHOWS_005384"
     CDS             complement(<1..>1190)
                     /gene="tssF"
                     /locus_tag="ACHOWS_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFQ0050787.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEIDIVLSEAWSSCLPFET
                     ENFRLHCAPVINLFTLEADPLLLNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVLNLTAPTMPLYPPANDRFHWRV
                     MSHLG"
BASE COUNT          273 a          325 c          353 g          239 t
ORIGIN      
        1 ccgaggtgac tcatcacccg ccagtgaaac cggtcatttg ccgggggata cagtggcatc
       61 gtcggtgcgg tcaggttcag gactctgacg gggactttgc cggttttgac caccctgtcc
      121 aggagcgtac tttccagcgc cctgcgcgga agctgcccgt tggtcgcggt gattcgcatc
      181 gagagggact ccggtttatc agacaactgc tccagttcga acgaacgtcc cccgaggact
      241 aaccatgtgt catacaggcc ggagggtccc cgtttcacgc gggtgtggta ataacgttca
      301 ggagcatcgt ggcgcatcat accgccccgg tgccggaagc tggtgaacgg gacataaggg
      361 tgtctgccat tcttcaccga gccgtgaatg ttatcgacgc tgtaaatttc ggtgtgacca
      421 tcctgaagac gcagcggcct gagcaggtac tcgttatcca gcgggttaag gagcagcggg
      481 tcggcttcca gggtaaagag attgataacc ggcgcacaat gcaggcggaa gttttctgtc
      541 tcaaaaggca aacaggatga ccaggcttca ctgagcacaa tgtcgatttc aaaccaggta
      601 ctggccgcag tgagcccgat ggtgtcgagg ccgcgcagtt caacaaacat aaacttcgga
      661 cggaagctga aatactccag cagcaactgg taaccactga aggctgtatc ggctttcttc
      721 cacaggcagt cattgtcgtc aaagcccatg ggtctgcacc agccgtcaaa ctgatgacgt
      781 ccggtatatg tcccggcatg acgcgcatac atggcatgta cacgccgggt cattgccaga
      841 tgcagagcag cgcttaccgg cgcctctgca ttaagaaaaa tggcgacttt gtcgatcccg
      901 gctttactcc agtccacttt ttcagggcat tcgaagcgca ggcggatggc agagcggcca
      961 tcggattcag tatggagacg ggcatctgca aggtgcagcg gttgtagcaa cacatcgcgg
     1021 gtcgtccggt actgacaggc ggttttatgc ggcccgacag gacgggacaa aacggagaag
     1081 ccttcagcca gtatttcggc ctgacgcaga ctacgccagt caggcgagaa ctcaacgatg
     1141 gccagcgacg ggatagtccg catatagtgc ggccacagca gactcaccag
//
LOCUS       DAZKJR010000380         1190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKJR010000380 DAZKJR010000000
VERSION     DAZKJR010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.383"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..528
                     /locus_tag="ACHOWS_005385"
     CDS             25..528
                     /locus_tag="ACHOWS_005385"
                     /inference="COORDINATES: protein motif:HMM:NF042725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFQ0050788.1"
                     /translation="MFRDIAADKAKLLLEKGGEIAVRFKIPENVNLETTKNKYAFGLY
                     WKTGEWPGEGGEGYLSSFFVQTDKASIDVAYHHTGNQQKELGTFGAFDHDWHTLAFKF
                     KGGNSINVTPVLDGQDGAAFDLVKWTNTANELNRFVVTDITANAETYPVLIDTVEVKV
                     NSVDTAA"
     gene            668..850
                     /locus_tag="ACHOWS_005386"
     CDS             668..850
                     /locus_tag="ACHOWS_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFQ0050789.1"
                     /translation="MTFIHQLMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            876..1148
                     /locus_tag="ACHOWS_005387"
     CDS             876..1148
                     /locus_tag="ACHOWS_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFQ0050790.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT          335 a          265 c          310 g          280 t
ORIGIN      
        1 cgggagagta caaaatcctg gtccatgttc cgtgatattg cagcagacaa ggcaaaactt
       61 ttactggaaa aaggcgggga aattgctgtc cggtttaaaa tccccgaaaa cgtcaatctt
      121 gaaacaacca aaaacaagta tgcctttggc ctgtactgga aaactggtga atggccgggt
      181 gagggcggtg aaggctatct gagttctttc tttgtccaga cagataaagc cagtattgat
      241 gttgcatacc atcatacagg taatcaacaa aaagaacttg gcacgtttgg cgcgttcgac
      301 catgactggc atacgcttgc ctttaaattt aagggcggta acagcattaa tgttactccg
      361 gtgcttgatg gtcaggatgg agcggccttc gacctggtga aatggaccaa tactgctaat
      421 gaactcaaca ggtttgttgt tacggatatc acagcgaatg ctgaaactta ccctgtgctc
      481 attgatacgg tggaagtcaa agtaaacagc gttgatacag ctgcataact tctgaaaaaa
      541 aacgccagcc acaacagttg cgctggcggt ggtaatcccc atggaggaag taggacatta
      601 gatttcgaac atcggctatg ttttaaacaa aaatagttcc tggtgtcaac aataacggtg
      661 agaaattatg acatttatac atcagttaat gctgtacttc tgtacggcgg tctgtgtgct
      721 gtatcttctt tcgggtgggt acagggctgt gcgcgatttc tggtgcaggc agattgataa
      781 aagggccgct gagaaaatca gcgccagtca gtcagccgga agcaaacccg aagagcccgt
      841 tactccttaa caaccccttt cagcgagaaa atcccatgtc agaaatcaca tccctggtca
      901 ctgcagaggc agtgaaggaa gtcctgcgct ctgaagaagt ccggagcgca ctgaaacaga
      961 aacttcgcca gaatcttgag gcgcgtcttg atgcagaagt tgatgccatt ctggatgagc
     1021 tgcttggtgt acaggcagag ccaccgactg aagcgggaga taccaccgca gagagcggtg
     1081 aagttcagcc tgaatcaccg gtcgccgatg cgactgaacc tcaacccgaa tcggtcatga
     1141 tgctgtaacg aggagtcagg gccatcagta aacggctgct ggcctttttc
//
LOCUS       DAZKJR010000381         1186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKJR010000381 DAZKJR010000000
VERSION     DAZKJR010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.384"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..577
                     /locus_tag="ACHOWS_005388"
     CDS             44..577
                     /locus_tag="ACHOWS_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFQ0050791.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCLPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            574..1041
                     /locus_tag="ACHOWS_005389"
     CDS             574..1041
                     /locus_tag="ACHOWS_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFQ0050792.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPRLADTVERDYFTLRERLMMMQKQLEGAQEYIRTQCLK"
BASE COUNT          290 a          290 c          342 g          264 t
ORIGIN      
        1 attaaagaag accggcgtaa ggcggcgcgg ggagagtaag ctgatgagca ggaaattccg
       61 ctatggttta tcagcggtcg ttctggcgat gattgctgca ggtgcttctg cgcctgaaat
      121 cctcgaccag tttcttgacg aaaaagaagg taatcgcacc acagcatacc gtgatggtgc
      181 gggtatctgg accatctgcc gtggtgccac ccgggtggat ggtaagcccg tcgttcccgg
      241 catgaagctg tcgaaggaaa aatgcgcaca ggttaatgcc attgagcgtg ataaggcgct
      301 ggcatgggtg gagaaaaaca tcaaagtgcc actgaccgaa ccccagaaag cgggtattgc
      361 gtcattctgc ccgtataaca ttggccccgg taagtgtctc ccgtcgacgt tttataagcg
      421 gcttaatgcc ggtgatcgta aaggtgcctg tgagtcgatt cgctggtgga ttaaggacgg
      481 tgggcgtgat tgccgcacac gttcaaataa ctgctacgga caggttattc gtcgtgacca
      541 ggaaagcgca ttagcttgtt gggggataga tcagtgagaa gagtaaccgc gattatttac
      601 gctctggtta tctgcatcat cgtctgtctg ttatgggctg ttaatcatta ccgcgataac
      661 gccatcacct acaaagcgca gcgcgataaa aaagccagag agctggagct ggcaaacgca
      721 accattactg acatgcagca gcgccagcgt gatgttgctg cgcttgatgc cagatactca
      781 agggaattag ccgatgcgag agctgaaaat gaaactctgc gcgctgatgt tgccgctggt
      841 cgtaagcgcc tgcggatcaa cgccacctgt ccaggctccg tgcgtgaagc ccccaccacc
      901 tccggcgtgg ataatgcaac cggcccccga ctggcagaca cagttgaacg ggattatttc
      961 accctcagag agcggttgat gatgatgcag aagcagctgg aaggggcaca ggaatatatc
     1021 cgcactcagt gcctgaaata agttttgctg atgcgccgta tcgtcgccgt attcccgtat
     1081 taacagcgac tgcagccctg acgggtacct cctgcgtgag tgtgcgggga taatcaaaaa
     1141 cgatacacac cgggtttccc tcatttttca ggagacgtga gcattt
//
LOCUS       DAZKJR010000382         1184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKJR010000382 DAZKJR010000000
VERSION     DAZKJR010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.385"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..254
                     /locus_tag="ACHOWS_005390"
     CDS             <1..254
                     /locus_tag="ACHOWS_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050793.1"
                     /translation="TGMEEMQDKMAWMQNLITEKLGPSLWHMHPIAFPGALIKKNTGW
                     AHSKFANLLGSVESENDYSAYNVTKTGKSVPHYNINLTK"
     gene            425..1003
                     /locus_tag="ACHOWS_005391"
     CDS             425..1003
                     /locus_tag="ACHOWS_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050794.1"
                     /translation="MGCNKKSNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIHFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGTPVGLPFEIDTYKNSSMIAITGQSISVS
                     KSDNPVCKTTLFNFNNNEYVKLIESLDGCNNQ"
BASE COUNT          400 a          186 c          251 g          347 t
ORIGIN      
        1 gtaccggaat ggaagaaatg caggataaga tggcctggat gcagaatctg atcacggaga
       61 aactgggccc ttcgttgtgg catatgcatc cgattgcctt tcctggagca ttaataaaga
      121 aaaatactgg gtgggcgcat agcaagtttg caaacctttt gggaagtgtc gagtcagaaa
      181 atgattattc ggcatataat gttactaaga caggtaaatc tgtcccgcac tataatatta
      241 atctcacaaa atgacattat ctgaggtaat tgatgcccca agagaacgaa aaatggtaaa
      301 aagaaataca ggttagtacc tagggtggca gaagttgagg gggagtcgta ctattctggt
      361 gatggattga ataaagctca cctattacca gatacgatga ttaaagctct tgaggattct
      421 aaaaatggga tgcaataaaa aatcaaacaa atttttgctt tttgtgtcat gcatgctctt
      481 gtcgtttgta acgtactcac aagacagttc taatcctcag ttttctgact acctggttcc
      541 tgtaagcaac ggaccatttg aaaagaatat tcactttaat aaagagcagg aaaattactc
      601 gcaacactgg aagaatgcag tccaagagga actaaaaaag cctgttaatt ttgcagggca
      661 ctttagaatc tatacagcct ttggcggtca tggtaaagaa tgccttcgtg ataattgggt
      721 atgtggttgg gttattgata aactatcagg ggaagttgta gcaacacttc catcagataa
      781 taatggaagt tataactatg ctgatgtgtc tgataatggt acccctgttg gtcttccatt
      841 tgagattgat acttataaaa atagttcaat gattgcaata accggtcaat ctatctcagt
      901 aagcaagagt gataatcctg tatgtaaaac gactttattt aattttaaca ataatgaata
      961 tgtgaaatta attgaatcat tggacgggtg taataatcaa taaatctctc atgaatattc
     1021 atcataatga gaaaacgact gtagtaaaat tcggatgcga catgtatcag tgaacgttca
     1081 gggttgagtt aatagattcg actctaaatc cgtgataaat gttaatttaa gctaagcaaa
     1141 tggacttaag aaaaacatgg cttatttgca ttctcacatg taat
//
LOCUS       DAZKJR010000383         1181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKJR010000383 DAZKJR010000000
VERSION     DAZKJR010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.386"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1181
                     /locus_tag="ACHOWS_005392"
     CDS             <1..>1181
                     /locus_tag="ACHOWS_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0050795.1"
                     /translation="TFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQRSITAPDRIG
                     EGKTWVYQRDTMVERGDNRGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYELAA
                     DVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAILHA
                     FNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTI
                     HNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDI
                     ESGIPGYWQGQVLIPFTIDPVTKERKQIHPEDIHLTAKDFETSKDNISKDEWENLHAL
                     KEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPL
                     T"
BASE COUNT          424 a          242 c          216 g          299 t
ORIGIN      
        1 caacttttca cgcagaaaaa gtcattaatg atctgttaaa aaacgattat aaaatttccc
       61 tttctccaga tacaacttac cgagagctga ataaagctgc acagcgtagc attacagcgc
      121 cagacaggat aggagaaggg aaaacatggg tttatcaacg agatacaatg gttgaaagag
      181 gtgataacag aggcgttcat cagtatggtc cagctgaaca tttcactcac attatatctg
      241 acaaaccatc cccaaaagat aagtatgttg catatgctat taacattcct gactatgagc
      301 tagcagccga tgtatataat attaacgtga cgtcaccttc cggacagcaa gaaacattta
      361 aaatactaat caatccagaa catctacggc aaacacttga gcgtaaatct cttactgctg
      421 ttcagaaatc acaatgtgaa atcatcaccc ccaaaaaacc tggcgaagcg attcttcatg
      481 cttttaatgc cacctaccag caaatcaggg aaaatatgtc tgagtttgca cgttcccatt
      541 atgggtatat acaaattcct ccagtgacaa ctttccgcgc cgacggacca gaaactcccg
      601 aagaagaaaa aggttactgg tttcacgctt atcaacccga agatctttgt accatccata
      661 atccaatggg agatttgcag gattttatcg cattggttaa agatgctaaa aaatttggta
      721 tcgatatcat tcctgattat acctttaact ttatgggaat tgggggtagt ggtaaaaatg
      781 acctagatta tccctctgct gatatacgag cgaagatcag taaagatata gaaagtggta
      841 tccctggcta ttggcaaggt caggttttga ttccatttac tatagatcca gtaacaaaag
      901 aacgtaaaca aatccatcca gaagatatac atctcactgc aaaagacttt gaaacaagta
      961 aagataacat ctctaaggat gaatgggaaa acctccatgc attaaaagaa aagcgtttaa
     1021 atggaatgcc taaaacaacc cccaaaagtg accaggttat tatgttgcaa aatcaatacg
     1081 ttcgtgaaat gcgaaaatat ggcgtacgag gtttacgtta cgatgcagca aaacactcaa
     1141 aacatgaaca aatagaaaga tcaataactc caccgcttac a
//
LOCUS       DAZKJR010000384         1177 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKJR010000384 DAZKJR010000000
VERSION     DAZKJR010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.387"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(355..927)
                     /gene="hdeD"
                     /locus_tag="ACHOWS_005393"
     CDS             complement(355..927)
                     /gene="hdeD"
                     /locus_tag="ACHOWS_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFQ0050796.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT          413 a          256 c          222 g          286 t
ORIGIN      
        1 gagacacgga tatttatgca atgtaatcaa cgccagttgc aaaaaaatga acccggatta
       61 cttatttaac cgcccatagc atcaacgctt acggaactct ttgttcactt tcccacagca
      121 cttttaaaac attaaacctg accacggaaa atatcagcca tgataatgtt tgaaatggct
      181 aatttgccat ggaacgaaaa aaatcagatt aaattcagta ggttgaaata atcaacagca
      241 ggcaattgtt tcaatgatag tgcgtaattg aactgaaaca ctgtatagct aagagagtat
      301 taactggatc atagtcactt aatgtgacta tgatccaggt ggaaataggg ataattattg
      361 ctttttaacg aacaaactgg cgaagctgaa cagactggcg gcgctaaata tcagttcaat
      421 tcccaccagt gtggaaacca gcgttaccga caccatcggc gtagcaccaa ggaatatcca
      481 ggcaatgacg atatccagca caccaataac gagctgtagc caactgcctt tcattgaacg
      541 ctgacgatac caactcatca ggcgaataac ccctgccaca cagaacaaac cggcaataaa
      601 tgccgcaatg gcaaaaatgc ccagctctgg tgcgcggatg aagaaatagc cgatcaataa
      661 ataggcgact gcgacgagga aaccggataa taccggccag aaattatgac tgcggttgct
      721 gaatagcccg acaataagcg caatgcccga gcagattaat aatgcaccca ctactgtgct
      781 taaaatgtcg ccagagacga acggaaaact gatacacagc aacccgacga taaacagcag
      841 cacggcaata aactggattg ctctgcgatg ttttttaagc atctccagat caaacttcaa
      901 aattgttgcc ttatctatat ataacataga accaccctat aaaattaaga agaaaatccc
      961 ctgctatcaa tctatgccaa aaacgcgtgt aagaatgcag tcgatttaat aaaaatttcc
     1021 taattacagt atctgatgcg tctgtaactc attgtattga aataaaaata tctgattttg
     1081 atattttcca tcaacatgac atatacagaa aaccaggtta taacctcagt gtcgaaattg
     1141 attcgtgacg gctctttcac tttgtagatg aggatat
//
LOCUS       DAZKJR010000385         1174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKJR010000385 DAZKJR010000000
VERSION     DAZKJR010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.388"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..186)
                     /locus_tag="ACHOWS_005394"
     CDS             complement(<1..186)
                     /locus_tag="ACHOWS_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0050797.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRR"
     gene            complement(254..>1174)
                     /locus_tag="ACHOWS_005395"
     CDS             complement(254..>1174)
                     /locus_tag="ACHOWS_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HFQ0050798.1"
                     /translation="QLAALMAGQLLPGEAGAWLATVPGALQNLAGAVWHQHPEMALIR
                     TVLQEAMEEVVSPFAAGDVAEVPVAEVVPVLLQSAAISAESVIQPLPVVPSPVEDAEN
                     EGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPDKESSIELPEESGC
                     VTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLSVNSVDSNVHVVSG
                     YVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFFQARLYSDAEHSGR
                     YKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
BASE COUNT          287 a          372 c          227 g          288 t
ORIGIN      
        1 acgccggtat cgctgaacat cacggacagt cacctccccc ggcaaaatat caccgataaa
       61 ttccctgaat accctgacaa ctttgcagta gctccactct gtatccggcc gaagattctt
      121 tgaaaaaaag tactcttcca gcagttgttc ccaggtgatt tttgtgacgg aatcgtcatt
      181 ctgcattata tattcccctt gtttactgtg ctcccgcact ctccggtcaa attctgcaca
      241 tcatcgcctt tactcacccg aaaacaataa gcgggctgtc tccgggaacc gtaatattcc
      301 cgtacagaaa cctgccctta accagatatc ccgaaaccat tttgtaacgt ccggaatgtt
      361 ctgcgtcact ataaagacgg gcctgaaaaa aacgctctcc tgaacgtacc ttatggatac
      421 ctgcccgttc aaagagcgac tgaacataac gacgatcctg cccggaacca ctctccttca
      481 gaaaacgata aaaaatcccc ggggcacaca gaaaaacata tccggaaaca acatgaacat
      541 ttgaatcaac gctgttaacc gaaagtgaac cctccctcag cccctctttt aaccatctga
      601 taaattcact ctctaccctg ctatcctctg tcagggaaaa ctctgccgtt ttttccactg
      661 tttccgggat acggtttccc tgattttctg ttacacatcc gctctcttcc ggtaactcaa
      721 tactgctttc tttatccggc ggcacgcctg acgctatctg ccctccctca tccgacaaat
      781 cagcagccga aaacagcgta aggagcattc cggtatcagc atcagtttcg ttcccctcat
      841 ccacctcctg ttccgctgtc cggccctcat tttcggcatc ctccactggc gacggtacaa
      901 ccggcaacgg ctgaatgacg ctttctgctg aaattgctgc tgactgcagc agtaccggaa
      961 caacctcagc caccggaact tcggccacat ctcctgcagc gaagggactc acgacttcct
     1021 ccatagcttc ctgtaatact gtacgtatca gcgccatttc aggatgctga tgccagactg
     1081 ctccggcaag attctgtaat gcccccggga cagtggcaag ccaggctccc gcctctccgg
     1141 gcagcagttg tcccgccatc agggctgcca gttg
//
LOCUS       DAZKJR010000386         1167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKJR010000386 DAZKJR010000000
VERSION     DAZKJR010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.389"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..902)
                     /locus_tag="ACHOWS_005396"
                     /pseudo
     CDS             complement(1..902)
                     /locus_tag="ACHOWS_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          279 a          338 c          299 g          251 t
ORIGIN      
        1 gccgcgtcac gttcaaagta ttgtttgtac tcagaccagg tggtggcggc aatggtacgc
       61 agttcacccc gcgccagcgc aggcttcagc aggttggccg catccgcgcc gccggcctga
      121 ttaccggccc cgataagggt atgggcttca tctataaaca acagtacagg agccgatgac
      181 tgctgcacgg catcaatcac gtttttcagg cgctgttcaa attcaccctt cacgccggca
      241 ccggcctgca gcaggccgag gtcaagggtg cgcagggtga cggggctgag tgattccggt
      301 acgttacctt ccgcaatacg cagggcgagc ccttccacca gagccgtttt ccccacacca
      361 gggtccccca ccagaatcgg gttgttcttg cggcggcggg agagaatatc caccatctga
      421 cggatttccg tatcgcgtcc cgataccggg tcaattttcc cctctctcgc gctggccgtg
      481 atgtcccggg tgaatttatc cagagcactc tgtaacacag gcggcaggtc acctgacggt
      541 atacccgccg tatccgcacc cacgggacgc ccgataaagg tcacattttc tccctgaggc
      601 agcacaggct ctgcatcctg taatctctcc ggacactcat cagactgcgc atccagcagc
      661 ggggacagac gctgcaactg gctatgactc agtgtcagca gcggccacag cccgtcacag
      721 cgaaccaggt gcggattttc cgtcagggca cccagcagat gcacgctgcg gatatgttct
      781 tctccctgta atgaggccac catccaggcc tgtttcagca gggcattcag ggacggcgca
      841 agctgcgggc ggctgcgtac gctacggggc tggttatcca gccagccgag cagcgacttg
      901 taaccaggtg aatcatttga taccgacaag ggttttagtg aaaggaggtg aaatcacctg
      961 taagatgctg aaataaaaca ggtgatgtat acttcggacg gaaaactctt tttgataccg
     1021 actgctcaac aaatagtcaa aagatcaaaa aacggcgaca catttgatac tgctaattta
     1081 cccttaaaat cttcttcaaa acatcttcaa actgccaaaa tgctcacata atcgatctaa
     1141 ctataatcag tatgataatc atcatga
//
LOCUS       DAZKJR010000387         1162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKJR010000387 DAZKJR010000000
VERSION     DAZKJR010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.390"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(240..1043)
                     /locus_tag="ACHOWS_005397"
     CDS             complement(240..1043)
                     /locus_tag="ACHOWS_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050799.1"
                     /translation="MSADGISDLIKPEIFLVIFPGTTTTAKRGGAGWLNNKRVVFRVW
                     LYISKASYKKMGIFQRGSAPESIPYREYHFPSYPDTHEKRKKVLRDWISFMRSRFNIN
                     QIAQFCYRMENEWAFTFDNIHVPRHFREGTDDTEWSWNYIKKKRLFSLSGLQHIQPQN
                     NTERKLLINAFFDTCLIENNPADDIDIKNMLLRKMNAAFYKRKSRARARIHSRNNQQM
                     SVSSGTDKQVNIRLKDFQYQQLLFIAQKENKSKSEIIKELITRAYRAIQ"
BASE COUNT          325 a          211 c          243 g          383 t
ORIGIN      
        1 ttttttacat tgttgcattc ggatataatg tccaggttta gtttaagaga attattcttc
       61 agaatagaaa gtggacgtga taataataag aaaagggatc ttatacgcga atatgtaagc
      121 gatgtaacaa aagaatattc gttatgtata cctggcatag tcagtcggca cgataaaaaa
      181 tagtcagtat catcccgtgc atatgatgta tggtcgggat gaaatggtga acgtggtggt
      241 tattgtatcg cccggtatgc cctggtaatg agttccttta tgatttctga cttacttttg
      301 ttttctttct gcgcgataaa aagaagttgc tgatactgaa aatccttaag acgaatattt
      361 acctgtttat ctgttcctga actgactgac atctgttggt tattccggga gtgaattcgc
      421 gcacgcgccc ggctctttct tttataaaac gctgcattca tctttctgag taacatgttc
      481 tttatgtcaa tatcgtcagc agggttgttt tcaataaggc aggtatcaaa aaaagcatta
      541 atgagaagct ttcgttcagt attgttctga ggctgaatgt gttgcaggcc gctcagggaa
      601 aataatcgtt ttttcttaat gtaattccat gaccattctg tgtcatcagt tccctcccga
      661 aagtgtcgtg gaacgtggat attatcaaag gtgaatgccc attcattttc catacgataa
      721 cagaactgag ctatctgatt tatattaaaa cgggagcgca tgaaggatat ccagtcacgt
      781 aatacctttt tccttttttc atgggtgtca gggtatgagg gaaaatggta ttcacgatag
      841 ggtattgatt cgggcgcaga accgcgctga aaaatcccca tcttcttata actggcttta
      901 ctgatataaa gccatacccg gaacacgact cttttattat tcaaccaccc ggcgccaccc
      961 cgttttgctg tggttgttgt accaggaaaa atgacaagaa atatttctgg ttttattaaa
     1021 tcggaaatgc catctgcgct catgtttgta cctccattaa attattaaat actgacattt
     1081 tcatgatatg tcagtcaatt agttttgtga catgcccatg tcgtctcaga agaacaatga
     1141 cgagtctgta ttatgggata tt
//
LOCUS       DAZKJR010000388         1157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKJR010000388 DAZKJR010000000
VERSION     DAZKJR010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.391"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..254
                     /locus_tag="ACHOWS_005398"
     CDS             <1..254
                     /locus_tag="ACHOWS_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050800.1"
                     /translation="TNDDMKKSTGGIITGSKFIGLIIVDLEEEKFFVYQYSLNNRIKR
                     KFRAYMKIKKDSPVKAITQFTKVNILYWDEMLKQLMNKL"
     gene            347..724
                     /locus_tag="ACHOWS_005399"
     CDS             347..724
                     /locus_tag="ACHOWS_005399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050801.1"
                     /translation="MIKTLFDPETMSREDMLREIQLLRDSRDLALELVFGYPETKAAE
                     QIKLNDDEITLLKAKLPEHHFQWLAGDGDEALTVGRLGYFADLADDFSVLAGEIDPDL
                     EEPEEEVISRGPCGVVFCLIESD"
BASE COUNT          410 a          185 c          270 g          292 t
ORIGIN      
        1 aaactaatga tgatatgaag aagtctaccg gagggataat tacaggtagt aaatttatag
       61 gactaatcat tgttgatctg gaagaagaaa aattttttgt gtatcaatat agcctgaata
      121 acagaatcaa aagaaaattc agggcatata tgaaaataaa aaaagacagt cctgtaaaag
      181 caataactca atttacaaag gtaaatatat tgtattggga tgaaatgtta aagcaactta
      241 tgaataaatt atgacagaca aggcggggaa acccgcctta ctctgtatta ttacttgtgt
      301 tagcatagtt aaaaacaagc attaataaat taaacgagat atatttatga ttaaaacctt
      361 gttcgatcca gaaaccatga gcagggagga tatgcttagg gaaattcagc tactgcgcga
      421 ctcacgtgat ctggcgcttg aactggtgtt cggctatcca gaaacaaagg ccgcagaaca
      481 aattaaattg aatgatgatg aaataaccct tctgaaagcg aaactaccgg aacaccattt
      541 tcaatggtta gcaggagatg gagatgaggc gctaacagtt gggcgattag gttattttgc
      601 agatctggcc gacgatttta gcgttctggc cggagagata gatccagacc tggaagaacc
      661 agaggaagaa gtgatatccc gtgggccttg tggtgtcgta ttttgtttga ttgagtctga
      721 ctgatcttgt gtgtgaggtg tagtaaatgc cgcgtctgcg gcatgggagg cgacaaaaac
      781 aagttaaata taaattggat gtaaagtgtc gccgtgacgc ggctacgccg ctcggatgta
      841 aataaatcac aggaacacct ccccaccctg ccagacgtgt ttgtgtctgg cgcagaatgc
      901 gccagacaac catatatcag gcaacacgtt acttaatggt cgtagacaag caattgtaac
      961 agtaacaggc ttgaatttta tggagatgat gaaatgggag attggcatac taagccaaga
     1021 acaggagcct ctcaaattgc tgagtatgaa gaggaaataa gattattaaa ggaaaaacaa
     1081 tatacgaaca aacaaatata tgaggtaata tcccagaaag ggatgacaat aggacagcaa
     1141 atgtttaata tatatat
//
LOCUS       DAZKJR010000389         1143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKJR010000389 DAZKJR010000000
VERSION     DAZKJR010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.392"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..917
                     /locus_tag="ACHOWS_005400"
     CDS             <1..917
                     /locus_tag="ACHOWS_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFQ0050802.1"
                     /translation="VIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQD
                     HATLKEIKDLAKNITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCY
                     SPLCKSASRMDQNLRAPVPMTWAFMMREAGFPAVRPKYELPLSKDVLDEVREEMRSLG
                     SYIALNLEGSSQERTFSLSIAENLIEKIQSETDIPIVIVHGPKGEEKARVLVDCYNNV
                     FRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVS
                     ESVVVGQKIDNISLDEFAKALKSVLLRI"
BASE COUNT          366 a          213 c          251 g          313 t
ORIGIN      
        1 ctgttattcg ggcgcttgaa ttgcataaaa ttaagcatct tttaattgta acgtcgacaa
       61 ttaatttaga agtctttaat gcgctcaaac ttgaacagac taaattaaca ttagttacaa
      121 tgactatgca ggatcacgca acattaaaag aaataaaaga tctagcgaag aacataacac
      181 agcaatacgg tacgccggat ctttgtatag aggcgatgcg taaaaagaac ctgaaaacga
      241 tgatatttat cagtcagttg aaagcaaaaa cgaattttca ggttgttggt ttaaccatga
      301 agtgctattc ccctttgtgt aagagcgcgt cccggatgga tcagaatctc cgggctcctg
      361 tacccatgac atgggcattt atgatgcgtg aggcgggttt tccagcagtc aggccaaaat
      421 atgaattgcc actaagtaag gatgtactcg atgaggtgcg cgaggaaatg cgatcgttag
      481 gatcttacat tgcgcttaat ttagaaggta gctcgcagga acgtacattt tcattatcga
      541 ttgcagaaaa tctaatagaa aaaattcaaa gtgaaacaga tataccaata gtgatcgttc
      601 atggaccaaa aggcgaagaa aaagccaggg tgttagtgga ttgttataat aatgtcttcc
      661 gtttatcctt atcaccctcg attaaacgtt cggcagcaat cataaaagat gcttatatcg
      721 caataactcc tgacacctca atattacata tggcaagtgc ctataatacc cccgttgttg
      781 caatttatgc tgattacaaa acgcgatggc ccgcaatggc cgatgtctcg gagtcagtcg
      841 tcgttgggca aaaaattgac aatataagtc tggatgaatt cgcaaaggca ttaaaaagtg
      901 ttttgttgag aatatgaggc agtacttatg catagtagtg ccgatctgtt atgaacggtg
      961 gtgtcatcaa tactttgtgg ctggtcaatc attacagaaa gccagccttt ggatcaaagc
     1021 tgtactcacc ggatacaacc atagtcacac cgaacgcgaa tctggcggta tcgcattttt
     1081 atttattcat tttctgtgag agaagggcaa gttctgtaga gagaatgtaa aaaagagcgc
     1141 ccc
//
LOCUS       DAZKJR010000390         1137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKJR010000390 DAZKJR010000000
VERSION     DAZKJR010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.393"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1137
                     /locus_tag="ACHOWS_005401"
     CDS             <1..>1137
                     /locus_tag="ACHOWS_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFQ0050803.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNLHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDMQGHEMRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TQISHLSEGHRVTVHYRYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRC
                     IPDSLPAVEWLTYGSGWLAGMKLGDTPLVDFTRDRLHRETLRSFG"
BASE COUNT          276 a          337 c          338 g          186 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacaccggat ggcagggcat cggcatttta ctataaccac
       61 cacagccagt taacgtcagc caccgggcct gacgggctgg aattgcgccg ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatctac acagtgactt accctgcgca acggaagatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacat gcagggccat
      421 gaaatgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggcttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac acaaatcagc
      841 catctcagcg aaggccaccg ggtgacggtg cattacaggt atgatgagaa aggccgcctg
      901 accggtgagc gccagacggt gcatcacccg cagacggaag cactgctctg gcagcatgag
      961 accagacatg cgtacaacgc gcaggggctg gcgaaccgct gtataccgga cagcctgccc
     1021 gccgtggaat ggctgactta cggcagcggc tggctggcag gcatgaaact cggcgacaca
     1081 ccgctggtgg atttcacccg tgaccgcctg caccgggaaa cgctgcgcag cttcggc
//
LOCUS       DAZKJR010000391         1128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKJR010000391 DAZKJR010000000
VERSION     DAZKJR010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.394"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..546
                     /locus_tag="ACHOWS_005402"
     CDS             <1..546
                     /locus_tag="ACHOWS_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFQ0050804.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLCSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGVDLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..>1128
                     /locus_tag="ACHOWS_005403"
     CDS             562..>1128
                     /locus_tag="ACHOWS_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050805.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVIAYRICRDYLT
                     IAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYESEAENQWVVQD
                     TLKAYSEMDWLTAKLTSDETSPQIAGYMP"
BASE COUNT          294 a          255 c          307 g          272 t
ORIGIN      
        1 attaggtttg tgaccgacaa aatcagggaa cttcagtctg cggtcctgct gggtagtgcg
       61 ccgcaggggg tggccctgac ctcaggtgaa caccttcagc tctgcagtac ccgtaacacc
      121 atgatcaatg caggccagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caaccaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tctctgaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaactctgac gctgaacggt
      361 ggtgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg tagatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt ggggggagaa
      661 tctaccgggc tgtacatgat aggtcgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccggttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgatt gcatgggaat ccggtccgct gaatttttcc
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac cagtggactg
      961 catttctata ctctgtatat gcatcttgcg ccgtattcag cttatgaatc tgaagcagaa
     1021 aatcagtggg tcgttcagga cactctaaaa gcatattctg aaatggactg gctgacggcg
     1081 aaactgactt cagatgaaac ttctccacaa attgcagggt acatgcct
//
LOCUS       DAZKJR010000392         1108 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKJR010000392 DAZKJR010000000
VERSION     DAZKJR010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.395"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..592)
                     /locus_tag="ACHOWS_005404"
     CDS             complement(<1..592)
                     /locus_tag="ACHOWS_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HFQ0050806.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDA"
     gene            824..>1108
                     /locus_tag="ACHOWS_005405"
     CDS             824..>1108
                     /locus_tag="ACHOWS_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050807.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIR"
BASE COUNT          296 a          302 c          252 g          258 t
ORIGIN      
        1 tcgcatcctg cgcctcatca aacaggatgg tgtcccatcg gtgagacagg tctgcaccgg
       61 acaactggaa gagtttcaga taagtatcgt gcgtcaccgg gaacgtcccc ccggggttgc
      121 tcatttcgtt ccagagcaac tgcaccgccc gaagcgcatc tgctggcgga acaccccgca
      181 tctcatcctt atccggcagg tgatgcaggc ccggtaccgc atcggcagaa cagataaagt
      241 gattcagccc cgtccctgcc acccgtgcca gagcccagta tcgtgtattc agctttctcg
      301 ccacatcagt aatacgtaat cccggaacca gacgatgacg gtagtgtctt ccaacccggg
      361 catacgccag ctgatgtgac gttctgcatt ccacgttaaa gggaaattta cgctctgcct
      421 catcacgtat tgcccggtta taggcaagat agagcatacg gctgtccgga ctggcccggg
      481 caaactgtac cagcgtactc gtcttccctg tgccggaaaa cgcattcaca accagttgct
      541 gcccctgcca gttaataatg gcgtactgtt catctgtggg attaaaagac atgtattctc
      601 ttctccggaa aaacatttcc gtaatctgat gcatcagggg tatgcccacc acaaataact
      661 ctgtacagca acagaaaaat ttccctggtc atatgatgaa ttaatcatcg ccatcacacc
      721 ggatgcttct catcctgtcc tcagaaaggc aggtggtctc ctgtctgccg cgcttatcca
      781 ggataccggg acctgaaagg tcataatctg gcaggaaaaa ataatgtccg aagtaaaaaa
      841 acacgtttct tattcattaa cagttgcagc tatgctgtca gccgccatcg tctgtcagac
      901 agcacatgct gatgtcaaca caccgaacat caccggagaa aaaaacaata caacaacacg
      961 attctccgga aatatttacg gcagtgaaaa tactgtcaca tcagagtcaa acagtctggt
     1021 tgtgggtaac agtaattccg tggagggagg gtataacaac attgttattg gtaattcctc
     1081 cacggtcaaa gcttccggtt tgatcaga
//
LOCUS       DAZKJR010000393         1075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKJR010000393 DAZKJR010000000
VERSION     DAZKJR010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.396"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..318
                     /locus_tag="ACHOWS_005406"
     CDS             76..318
                     /locus_tag="ACHOWS_005406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050808.1"
                     /translation="MMMARDITAYHLNSVHISARRFPGIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
BASE COUNT          285 a          206 c          252 g          332 t
ORIGIN      
        1 atccagaagt cctgctgagc cttccgtctg ttcatcatgt gccaggtttg cgttcgggat
       61 atgatgtgcc atcttatgat gatggcaaga gatattactg cgtaccacct caattctgta
      121 cacatcagtg cccgtcgttt ccccgggatt tttcagtggg ccaccggtat gcaaacaaaa
      181 aatctgccgt taactaattc tttgttacag aatgattacg cagaaagtat ttttgtattt
      241 aatggcgaac gtgacgtttc ggaaatgcct ttaccggaag aaatgctact gactggaaac
      301 gctgagtcag aaagataatt cggtgtattc atgactgtta tcaatgtttt atgtgtatga
      361 tgtcaacgac ggatgaaaag ttatccactt atatgaggca ttataggatt atttctggct
      421 gacgttgttt cagttcagac caggggcagt gaatatgctg acctgctttt tcgctgatat
      481 cagtccccct gtaattacat agataataga tatacctctc cgttgatcag gctgtccagg
      541 taattgatac cgcacttcct catcagttcg tagcagagtg tacttagcat ggctagtgca
      601 gtttctaatg atttacagtg tgtattcagg agagtaagaa attgctcaaa acatcctggt
      661 ttatactggt gctttgatat tcattgtgtc ccttttccgt tcgttattat ttccatattt
      721 aatatgatgc ctgtacatgt aaaataatgg aagatttcag gggagcattg cataaagtcc
      781 gcctggagaa taaggctcag gtcagcgaat cgcatcgtgg ccagactgca ctgattgcaa
      841 taagtgcgga tacagagttg cagctactcg tcgatatcag aacaacagaa tccttgcaaa
      901 cagggatgtg ttcattcagg gggtatgatg ctacgacgaa cgcttttagt gattattaat
      961 cgacgatact gtgttttgtc tggatggtga gggactcgct ttgaggattc accagggaaa
     1021 tacggggctt gtggcaataa tgttagcctg cgaacttaaa tgtttacggg caaca
//
LOCUS       DAZKJR010000394         1074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKJR010000394 DAZKJR010000000
VERSION     DAZKJR010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.397"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            155..1036
                     /gene="htpX"
                     /locus_tag="ACHOWS_005407"
     CDS             155..1036
                     /gene="htpX"
                     /locus_tag="ACHOWS_005407"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFQ0050809.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT          258 a          246 c          298 g          272 t
ORIGIN      
        1 acagcagcaa gatgccgtaa atcagatgct acaaaatgta aagttgtgtc tttctggtga
       61 cttacgcact atccagactt gaaaatagtc gcgtaaccca tacgatgtgg gtatcgcata
      121 ttgcgttttg ttaaactgag gtaaaaagaa aattatgatg cgaatcgcgc tcttcctgct
      181 aacgaacctg gccgtaatgg tcgttttcgg gctggtactg agcctgacag ggatacagtc
      241 gagcagcgtt caggggctga tgatcatggc cttgctgttc ggttttggtg gttccttcgt
      301 ttcgcttctg atgtccaaat ggatggcatt acgatctgtt ggcggggaag tgatcgagca
      361 accgcgtaac gaaagggaac gttggctggt caatacggta gcaacccagg ctcgtcaggc
      421 ggggatcgct atgccgcaag tggctatcta ccatgcgccg gacatcaatg cttttgcaac
      481 cggtgcgcgc cgtgatgcct ctctggttgc tgtcagcacc ggtttgctgc agaacatgag
      541 cccggatgaa gccgaggcgg taattgctca cgaaatcagc cacatcgcca atggtgatat
      601 ggtcaccatg acgctgattc agggcgtggt gaacaccttc gttatcttta tttcccgtat
      661 tctggcgcag cttgccgcgg gttttatggg cggaaatcgt gatgaaggtg aagagagcaa
      721 cggcaacccg ctgatctact ttgcggttgc aacggttctg gaactggtgt ttggtattct
      781 ggcgagcatt atcaccatgt ggttctcgcg tcatcgtgaa ttccacgccg atgcgggttc
      841 agcaaaactg gttggtcgcg agaaaatgat tgctgcattg cagcgcctga aaaccagcta
      901 tgaaccgcaa gaagcaacca gcatgatggc tttctgcatt aacgggaagt cgaaatcgct
      961 cagtgagttg ttcatgactc acccgccact ggataaacgt attgaagctc tgcgtacggg
     1021 tgaatacctg aagtaatatg aagtaaatac taaaaagcgc gtcctgatcg acgc
//
LOCUS       DAZKJR010000395         1067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKJR010000395 DAZKJR010000000
VERSION     DAZKJR010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.398"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..1053
                     /gene="tdcA"
                     /locus_tag="ACHOWS_005408"
     CDS             115..1053
                     /gene="tdcA"
                     /locus_tag="ACHOWS_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFQ0050810.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT          299 a          196 c          243 g          329 t
ORIGIN      
        1 aataacgata ccctggaaag tattgaaagt taatttgtga gtggtcgcac atatcctgtt
       61 catttcattt tgatacactt catgccgtca atgaggtaat taacgtaggt cgttatgagc
      121 actattcttc ttccgaaaac gcagcacctg gtagtctttc aggaagttat tagaagtggt
      181 tctatcggct cggctgcaaa agagttaggg ttaactcaac cggccgtcag taaaatcatt
      241 aacgatattg aagattattt tggtgtggag ttagtggtgc ggaaaaatac cggtgtaaca
      301 ttaacacctg ccggtcaatt gttactctcc cgttccgaat ccattacccg tgaaatgaaa
      361 aatatggtta atgagataag cggtatgtct tctgaggcgg tggtggaagt ctcatttggt
      421 tttccttcat tgattggttt tacttttatg tcagggatga tcaacaagtt caaagaggtg
      481 ttcccgaaag cgcaggtttc tatgtatgaa gcgcaactgt cttcgttctt accggcaatc
      541 cgcgacggtc gactggattt tgcgattggt acgttaagcg cagaaatgaa gcttcaggat
      601 ttacatgttg agccgctgtt cgagtccgag tttgtgctgg tagccagtaa gtcccgaaca
      661 tgcaccggca ccaccacgct ggagtcgttg aagaacgaac agtgggtgtt gccacaaacc
      721 aatatggggt actacagcga actacttact acgttacaaa gaaatggcat cagtattgaa
      781 aacatcgtta aaaccgactc agtcgtgaca atttataatc ttgttctcaa tgctgatttc
      841 ttaactgtaa ttccttgtga tatgacatca ccttttggtt ctaatcaatt tattactatt
      901 ccggttgaag aaacattacc tgtggcacaa tatgccgcgg tatggtcgaa aaattatcgt
      961 attaaaaaag cagcatcggt tttggtggaa ttagccaaag agtattcatc ttataatggg
     1021 tgtagacgaa ggcaattaat tgaagttgat tagttatttg ttttaat
//
LOCUS       DAZKJR010000396         1064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKJR010000396 DAZKJR010000000
VERSION     DAZKJR010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.399"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..893
                     /gene="gadX"
                     /locus_tag="ACHOWS_005409"
     CDS             69..893
                     /gene="gadX"
                     /locus_tag="ACHOWS_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFQ0050811.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
BASE COUNT          313 a          192 c          235 g          324 t
ORIGIN      
        1 atttgactta agagggcggc gtgctacatt aataaacagt aatatgttta tgtaatatta
       61 agtcaactat gcaaccacta catggtaatt gtctaattgc gtacgcaaga cataaatata
      121 ttctcaccat ggttaatggt gaatatcgct attttaatgg cggtgatctg gtttttgcgg
      181 atgcaagcca aattcaagtg gataagtgtg ttgagaattt cgtattagtt tcaagagaca
      241 cgctttcatt atttctcccg atgctcaagg aggaggcatt aaaacttcat gtacataaaa
      301 aagttccttc attactcgtt catcactgta ccagagatat tcctgttttt caggaagttg
      361 cgcaactatc gcagaataag aatcttcgct atgcagaaat gctacgtaaa agggcattaa
      421 tctttgcgtt gttatctgtt tttcttgagg atacgcagtt tataccgtta cttctgaacg
      481 tgttgcaacc gaatatgcga acacgagttt gtacggtcat caataataat atcgcccatg
      541 agtggacatt agcccgaatt gctagcgaat tgttgatgag tccaagcctg ttaaagaaaa
      601 aattgcgcga agaaggtacg tcatattcac agttgcttac tgagtgtagg atgcagcgtg
      661 ctttacaact tattgttata tatggggttt caattaagcg agttgcagta tcctgtggat
      721 atcacagcgt gtcgtatttc atttacgttt ttcgaaatta ttatgggatg acgcccacag
      781 agtatcagga gcgatcggcg caggaattac cgaactgtgg ctcggcggca agtatggctg
      841 cgcaagggat tttttacggc actgaccgtt ctgcggaagg aataagatta tagagtttta
      901 ctcagacata aaaaaaaccc ggcatagggg accgggaaga ggatagtctg ccgtctccag
      961 actaataaac cgttataaca ctccctgttg gcacgggaaa ctttgtgctc tcagtaagtt
     1021 aaatataact tttactggaa ataagatcag ccattttttt ataa
//
LOCUS       DAZKJR010000397         1063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKJR010000397 DAZKJR010000000
VERSION     DAZKJR010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.400"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1063
                     /locus_tag="ACHOWS_005410"
     CDS             <1..>1063
                     /locus_tag="ACHOWS_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFQ0050812.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGTDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TGISHLSEGHRVTVHYRYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRC
                     IPDSLPAVEWLTYGSGWLAG"
BASE COUNT          258 a          311 c          322 g          172 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacgccggat ggcagggcat cggcgtttta ctataaccac
       61 cacagccagt taacgtcagc caccgggact gacgggctgg agttgcgcag ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatccac acagtgactt accctgcgca acggacgatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacac gcagggccat
      421 gaaacgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggtttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac aggcatcagc
      841 catctcagcg aagggcaccg ggtgacggtg cattacaggt atgatgagaa aggccgcctg
      901 accggtgagc gccagacggt gcatcacccg cagacggaag cactgctctg gcagcatgag
      961 accagacatg cgtacaacgc gcaggggctg gcgaaccgct gtataccgga cagcctgccc
     1021 gccgtggaat ggctgactta cggcagcggc tggctggcag gca
//
LOCUS       DAZKJR010000398         1059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKJR010000398 DAZKJR010000000
VERSION     DAZKJR010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.401"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..856
                     /locus_tag="ACHOWS_005411"
     CDS             <1..856
                     /locus_tag="ACHOWS_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HFQ0050813.1"
                     /translation="GIEEGDAREELSRKNELPFIGCDKQLRTITNSLKDNFSFQYLEA
                     ERFGPRDNIPLSQHNYHEGWLGKNGEHTIEVLESLVTRRRLNFSAQEDPRKHEGIINN
                     NVFNHIEAWMSEISPGHRISPRIEARANVAFNVIIPSPGQLTKPINIGFGFSYALSIV
                     TALMLASKNELVIIENPEAHLHPRGQSYLGRLIALSAKAGIQVIIETHSDHLLNGIRV
                     IARTDPTFDASLFTLYYISRENDETSVAKIKINNEGKLSEWPRGFFDQQSQDMYTIMT
                     GSFDHPNI"
     gene            876..>1059
                     /locus_tag="ACHOWS_005412"
     CDS             876..>1059
                     /locus_tag="ACHOWS_005412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050814.1"
                     /translation="MKAGVFLIPECFDFNTDILSLSSDLDLASKLVVNTRSEFIHFMK
                     SREFDSKIAELLFSTSP"
BASE COUNT          343 a          171 c          205 g          340 t
ORIGIN      
        1 tggtatagaa gaaggggatg ctagagaaga actttctagg aaaaatgaat taccttttat
       61 tgggtgtgat aaacaattaa gaactataac aaactcactt aaagataact tttcattcca
      121 atatttggaa gcggagagat ttggtccaag agataatatt ccattatcac agcacaatta
      181 tcatgaaggt tggcttggga aaaatgggga acatacaatt gaagttcttg agagtttggt
      241 cacaaggagg cgtttaaatt tttctgctca agaggatcct aggaaacatg aaggaattat
      301 taataataat gttttcaatc atattgaagc atggatgtct gagatttcac cagggcatcg
      361 tatctcacct agaattgagg ctagggcgaa tgttgcattt aatgtcataa tcccttcacc
      421 tggacaatta acgaaaccta ttaacatagg atttggattt agttatgctc ttagtattgt
      481 cactgcttta atgctggcat caaaaaatga actcgttatt attgaaaatc ctgaggctca
      541 tctacatcca agaggtcaaa gctatttagg acgacttatt gctttatcag caaaagcagg
      601 gatccaggtt attattgaga ctcatagtga tcatcttctt aatggcataa gagtaattgc
      661 acgcacagat ccaacttttg atgcttctct ttttactctt tactatattt cacgagaaaa
      721 tgatgaaaca tctgtcgcca aaataaaaat aaataatgaa gggaaacttt ccgaatggcc
      781 tagagggttc tttgatcaac aatcacagga tatgtatacg ataatgacag gctcttttga
      841 tcatcccaat atttaatatt gtagggacat ataaaatgaa agctggcgtg tttcttattc
      901 ctgaatgttt tgatttcaat acggatattt tatctttaag ttcggacctt gatctggctt
      961 cgaaactcgt agtaaatacc cgttcagaat ttattcactt tatgaaatct agggagtttg
     1021 actcgaagat tgcggagtta ttgttttcca catcacctg
//
LOCUS       DAZKJR010000399         1058 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKJR010000399 DAZKJR010000000
VERSION     DAZKJR010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.402"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..534
                     /locus_tag="ACHOWS_005413"
     CDS             <1..534
                     /locus_tag="ACHOWS_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFQ0050815.1"
                     /translation="IGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            531..>1058
                     /locus_tag="ACHOWS_005414"
     CDS             531..>1058
                     /locus_tag="ACHOWS_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050816.1"
                     /translation="MKRILFFLLMIFVSFGVIADCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTIWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECL"
BASE COUNT          359 a          200 c          225 g          274 t
ORIGIN      
        1 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt tcagtatata
       61 gattcaatgg gactggcatc aaaatatgga cacttaaata atggcggata tggagcgaga
      121 cccaacaaac cgcctacgcc cgatccaagt aaattgccag acatagcgaa acaattaaga
      181 ctgccatatc ctattgacca ggccagtagt gcgcctaatg ttttcaaaac attcttcaga
      241 gcattaagcc cttacgacta cacactgtat tgcaggaagt gggtaaaacc aaatctgact
      301 tgtacgccac aggatgatcc ccagtatcca gggatggata caaagacagc aagtgattac
      361 ctgccacaga caaattggcc aacaactcaa ttaccaccag gatatacttg tgcagaaccc
      421 tatttattcc cagacattaa taaacccgat gggccagcaa cagcagggat agatgatttg
      481 ggtgaaattt tagctaaaat gaaacagaga acatcgagag gaataagaaa atgaaaagaa
      541 ttttgttctt tttgctgatg atatttgtta gttttggtgt tatagctgat tgcgaaacac
      601 aagctaaaga tcaagattgc tttaccattt tcactaaggg tactattttt tccgcgtttc
      661 ctgtcttaaa taataaaacc atatggagat ggtatcagaa tgaggacata ggtgagtatt
      721 attggcaaac agaacttggt atatgtaaaa ataacaaatt cacaccaagc ggggcgagac
      781 tcttaattcg cgtcggatca ctacgcctta atgaaaatca tgcaaccaaa ggaacgttgc
      841 aggagttgct aaatacagca gaaaaaacgg cgtttctcgg cgatcggttt agaagttata
      901 taagagcggg tatctatcaa aaaaagagca gtgatccagc tcagttgttg gcagtccttg
      961 ataattcaat catggttaaa tattttaaag atgagaagcc aacttatgct cgaatgacag
     1021 cacatctccc gaacaaagat gagtcttacg aatgttta
//
LOCUS       DAZKJR010000400         1055 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKJR010000400 DAZKJR010000000
VERSION     DAZKJR010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.403"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..>1055
                     /locus_tag="ACHOWS_005415"
     CDS             20..>1055
                     /locus_tag="ACHOWS_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0050817.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIK"
BASE COUNT          352 a          163 c          237 g          303 t
ORIGIN      
        1 tacgttagca ggtgtcacca tgaaaaaagc catagcatat atgcgatttt catcaccagg
       61 tcagatgtct ggcgactcat taaaccgaca gagaagactt attgctgaat ggttaaaggt
      121 aaatagtgat tattatcttg ataccataac atatgaagat ttaggattaa gtgcattcaa
      181 aggaaagcat gcacaatcag gagctttttc ggaattttta gatgctatag agcatggtta
      241 tatattgcca ggaactacat tgttagttga aagtctggac agactttcaa gagaaaaagt
      301 cggtgaagcg attgaacgtc tgaaattgat tttgaatcac ggtattgatg ttataactct
      361 ttgcgataat acagtctata atattgactc tttgaatgag ccatattcat taataaaagc
      421 catacttata gcacaaaggg caaatgaaga aagcgagata aagtcaagtc gggttaaatt
      481 atcatggaag aaaaaacggc aggatgcact ggaatcaggt acgattatga cggcgtcttg
      541 tccgagatgg ctctccttag atgacaaaag aacggctttt gttccagacc ccgacagggt
      601 gaaaactatt gagctaattt ttaaactcag gatggaaagg cgctcattga atgcaatagc
      661 caagtattta aatgatcatg ctgtaaagaa tttctcagga aaagaaagtg catggggacc
      721 ttctgtaatt gaaaaattat tagcgaataa agctctgata ggtatatgcg taccttcata
      781 tcgtgcaaga gggaaaggga taagtgaaat cgctggctat tatcccagag tcatatcaga
      841 tgatttgttt tacgctgtac aggaaattcg gttggcacct tttggtatta gcaatagtag
      901 caagaatcct atgctaataa atctacttcg aacagttatg aagtgtgagg cttgtggtaa
      961 taccatgatt gttcatgcgg tatctggaag tttgcatggc tattatgttt gtccgatgag
     1021 aagattacat cgatgtgaca ggccatcaat aaaaa
//
LOCUS       DAZKJR010000401         1053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKJR010000401 DAZKJR010000000
VERSION     DAZKJR010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.404"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1053
                     /locus_tag="ACHOWS_005416"
     CDS             <1..>1053
                     /locus_tag="ACHOWS_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFQ0050818.1"
                     /translation="ATQAAEIYNKDGNKLDFYGRVKARHYISDGSMDGDRTFARLGFK
                     GETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAGIRHNILGSLDYGRNYGVAY
                     DVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDFFGLVDGLTFSAQYQGKNDR
                     DNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEKEGRKRFMTSKDSEGKEVIT
                     PRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPFGSAGIADKARNIEVVAQYM
                     FDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGANYFLNKNMSLLIDYKINLI
                     DESEFTKKAGVATDNIL"
BASE COUNT          305 a          194 c          259 g          295 t
ORIGIN      
        1 ggcaacacag gcagcagaaa tctataataa agacggtaat aaactggatt tttatggtcg
       61 cgttaaagcc cgtcattaca tctctgatgg ttctatggat ggcgacagaa catttgcacg
      121 tcttggtttc aaaggcgaaa cacaaattaa tgagagcctg actggctttg gtcagtggga
      181 ataccagttc ctggggttta agacagaaag tgaagtacgc aacgataaaa atcgtctggc
      241 atttgcaggt attcgccaca atatactcgg ttcacttgat tatgggcgaa actatggtgt
      301 tgcctatgat gtgggggctc tgactgacgt acttccggac tttggtggtg acagctggac
      361 ttactctgat aactatatga ccggcagaac aaccggagtt ctgacctggc gcaatacaga
      421 tttcttcggg cttgttgatg gacttacttt ttctgctcaa tatcagggta aaaatgaccg
      481 tgataattta cttgaagcta atggtgatgg atatggtttc tccgtaagct atgactatga
      541 aggttttgga atcgttgccg cttacactaa tgccgacaga accagtctgc aggaaaaaga
      601 aggccgtaaa cgtttcatga catcgaaaga ttcagaagga aaagaagtta ttacgcctcg
      661 ctggattgcc gggggaaaac atgcggagtt tgcaggtgtt ggggtcaagt ataatgataa
      721 tgacctgtat ctggcagctg actactctga aacccgaaat atgactccat ttggtagcgc
      781 aggcatcgca gataaagcac ggaatattga agttgtggct cagtatatgt ttgatttcgg
      841 cctgcgtcct tcactggctt acgttcagtc caaagggatg gatgttaaag gtggaatagt
      901 aaactatggc gatcagtctc ttgttgagta tattgatgtt ggcgcaaact actttctgaa
      961 caaaaacatg tcacttctta ttgattacaa aattaacctt attgatgaaa gcgaatttac
     1021 caaaaaggct ggagttgcaa ccgataatat act
//
LOCUS       DAZKJR010000402         1034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKJR010000402 DAZKJR010000000
VERSION     DAZKJR010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.405"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(739..>1034)
                     /gene="cdiI"
                     /locus_tag="ACHOWS_005417"
     CDS             complement(739..>1034)
                     /gene="cdiI"
                     /locus_tag="ACHOWS_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease toxin immunity protein CdiI"
                     /protein_id="HFQ0050819.1"
                     /translation="LMYNENRFLDAVENIVNKESYILDGIYCNFPDMNSYDESEHFEG
                     VEFAVGYPPDEDDIVIVSEETCFEYVRLACEKYLQLHPEDTEKVNKLLSKIPS"
BASE COUNT          299 a          192 c          223 g          320 t
ORIGIN      
        1 ttttattttt ccaacagtaa agcgaaaaaa caatggagaa ctcgaagaag cagcatagcg
       61 tttcgtagag ttagcccttg ctaagggatg attagttagt attgtttgat ggttattgct
      121 aagtcttcca tagttggtaa aattccgaaa tgttaaactt ttgataaatg gtattaaatc
      181 aaggggttat atggaaatca aagatgtttt cggggcgcaa cccaaaagtg tgtgggagta
      241 tatctgtgag aatggtcaag gattgtatgt gccagcatat caaaggcaat attcttggga
      301 taaacctcca ggttgtgatg ccagttccac catacgaagt ttgaattcat tcgaatagtt
      361 tttacgaggt tcttttcgcc agtcctgtaa ttccatactt agatgtccgt ctatatcaga
      421 tgggcgtcta agttaccaat tctcgtctga tggctacata cggcggtcag tttacgctta
      481 cggttttgac atcaattatc tccggtaatt tgatgaatga atgtcggtaa tgctaactga
      541 ctgaaaatcg ctgtgaagat gacttccccg taacgacaca acgtatgtca acaggccgca
      601 gatgctcacg tggtttaacg ccttccccgg tgcgcttaac gacattagag atgtgccggt
      661 gattaagcgt ggggatggac attgtggatg agcctgacgg ttaaagaaag atcccggcgc
      721 agtggccgga atctttactc aggatgggat tttagacagt aatttgttta ctttttcagt
      781 atcttcaggg tgtaactgaa gatatttttc acaggccaga cgaacatatt caaaacaggt
      841 ttcttcgctt acgatcacta tatcatcttc atctggggga tagccgacag caaattcaac
      901 accttcaaaa tgttcgcttt catcataact attcatatca ggaaagttac aataaattcc
      961 atccagaata taagattctt tgttaactat attttctaca gcgtccaaga acctattttc
     1021 attatacata aggt
//
LOCUS       DAZKJR010000403         1031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKJR010000403 DAZKJR010000000
VERSION     DAZKJR010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.406"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(75..638)
                     /gene="ipbA"
                     /locus_tag="ACHOWS_005418"
                     /gene_synonym="fimX"
     CDS             complement(75..638)
                     /gene="ipbA"
                     /locus_tag="ACHOWS_005418"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFQ0050820.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKCRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          314 a          182 c          202 g          333 t
ORIGIN      
        1 tgaatgagct gtttttttat ggaagaattg aggcaaacgt aggaatctga cattttgaat
       61 cagaaggtac tgttttaacg aggctttttt ttccacaccc ctttaaatcg agcagcatta
      121 ctggctgtgt atctgacagt atgctgaata tttcgatgac ccagataatc ctgaagtagt
      181 cgggtatcga caccattgtc agccagagca aatccacagg catgacgtaa catatgagga
      241 tgtgctttta cagctaatcc tgcacgtctt cctgcctcac gaatgatcga aaaaaaatgt
      301 tgtctgctga tagggcggca tttccgggag agaaaaagcc agtctccttc aactccattt
      361 tcaattaact tcctctgctt aagccacaac ttgatcaaat tatattcatc aggaagaaga
      421 ggatgtgttg tcgagaagcc atttttaata cgccggatat taagttgttt tcctgatgca
      481 tcaatatcgg ataaacgtaa atcaagaagt tcactggccc taaaaccgtg aatgaaagcc
      541 atcataatta gacagcgatt tctttcagga aaggacatgc tctgggctgc atccatcagc
      601 ctgtagactt catcctgtgt gagatatttt ctcgtcatac gccaccgtgt acagttgtaa
      661 aaaaaagagg cacacccaga taagtgtgcc tgcaatcaga acgttatcga tgtaatttca
      721 ttatctcatt aataagattt tttgaatagt agttctacgc aatagattaa atatgaaata
      781 tttattgtgc ggtttttcta caactcattt ttaatgcgcg attttttcta caatgatgtg
      841 aaacaaaaaa tattaaaacc ctgagtcaag gagcgggggt gaatgtgtat tacgtctaat
      901 gttttagtga tgttcaatag tcttgattta tttaatgtta tttttacgta aaataacttt
      961 ttctgcatat attattgatt ttatttgttt tttatgcgtt gcaaagtaag ggtgcagaaa
     1021 agaaaagatc t
//
LOCUS       DAZKJR010000404         1030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKJR010000404 DAZKJR010000000
VERSION     DAZKJR010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.407"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(158..637)
                     /locus_tag="ACHOWS_005419"
     CDS             complement(158..637)
                     /locus_tag="ACHOWS_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050821.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(720..>1030)
                     /locus_tag="ACHOWS_005420"
     CDS             complement(720..>1030)
                     /locus_tag="ACHOWS_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050822.1"
                     /translation="RNNLSYYSRWYTNPAGNLSVRVCMFRRGGVWTPCLYYRQGMYRN
                     EYTGWYQPLPREVIYLNRFTGRISRRRTWHAACRMAEQLTTLRYRFGLQGDGIVAQPC
                     "
BASE COUNT          277 a          298 c          237 g          218 t
ORIGIN      
        1 ggaacaaaac tcccgtcagg gaaaacaccg gagcaaaaac acggtgcagt aagtaaaaga
       61 aaaaccgtta cgggagcaaa acagccatca ttgcaaaatg gcagttgcat cgctcacgat
      121 ttgacatcct tcctgtgact gactggcatc ctgcccgtca gtctgcgtcg gaaaattcaa
      181 tacaggcctg cttcaggagc ccggctatac gttgattcag acattccgtc cagccatcgt
      241 tccatccacg ctcccatgga ttgcagacat cctcccatgg attgcagaca tcgttacagc
      301 cattcttcca tgtcttccat gcctccatct ccatgacagc ccccagcctg tggcctttca
      361 cacgtatatg ccacagaaac ggccggaaaa gtcctgattt cagttggtca ccaccgcttt
      421 tgtagcccag atgacgtgca atactttcag tatattcaaa tgcatcattc tgcattccag
      481 ccgccttagc cagcgagctg tacgcatcac gaagtatcgt cataccttcc agttgatact
      541 cacaccacgc acggacagcc gacactggtg cgattacccg aagaatggtg caggcacgcc
      601 atacaacaac gtaaaggagt attaacagac tatgcatgtg ttcacctcag atacaaaaaa
      661 taacgggaac acacctgccc tgcgggaagc gtgttcccga cagggtaata aatgataaat
      721 cagcagggct gtgcgacgat tccatcaccc tgcagaccaa accggtaacg cagggtggtc
      781 agttgctccg ccatacggca ggcagcatgc catgtcctgc gacgggatat ccgtccggta
      841 aaccggttca gataaatgac ctcacgcggt aacggctgat accatcctgt gtactcgttg
      901 cgatacattc cctgccggta atacaggcag ggtgtccaga caccgccacg acggaacata
      961 cagacccgca cgctcagatt gccggccgga ttagtgtacc agcgactgta atatgacagg
     1021 ttattgcggg
//
LOCUS       DAZKJR010000405         1026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKJR010000405 DAZKJR010000000
VERSION     DAZKJR010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.408"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..402
                     /locus_tag="ACHOWS_005421"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACHOWS_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            397..777
                     /locus_tag="ACHOWS_005422"
     CDS             397..777
                     /locus_tag="ACHOWS_005422"
                     /inference="COORDINATES: protein motif:HMM:NF026977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050823.1"
                     /translation="MNKTVEFSRGPQTYVSDLGHVTGSTARARNKAITAILKEDFAYL
                     KLTYIPQYNPFMRTGIAKLWEGTQFGKNSFSSRAALRDVIIHEELHHRWWKRGLNDHH
                     PKGTEMEQKFYETIRRYKRMRGWL"
     gene            777..>1026
                     /locus_tag="ACHOWS_005423"
     CDS             777..>1026
                     /locus_tag="ACHOWS_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050824.1"
                     /translation="MFGDEFKTTIECPCCGKICSIAIGQTSHNGILAWYESIICHNCG
                     LRSESDDIGFPPEKIRLQLIDFEGLWNVRLENIKSQMET"
BASE COUNT          288 a          200 c          276 g          262 t
ORIGIN      
        1 ccgtccgtga ccccgcagtg ggtgacgcgg acgaattatg cggtgagcga cctgacaggc
       61 cgcccgctga tgctctttaa cagtgaaggt aaaaccgtct ggcggccggg acagaccagc
      121 ctgtggggac tggcactcag cctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gatccgctgg ggttgctggg gggtgagcag acttaccggt atgtgcctaa tccgtgtggg
      361 tatgttgatc cgctgggatt agctataact agtataatta ataaaacagt cgagttttct
      421 agagggccac agacttatgt ttctgatctc ggtcatgtta caggttctac ggccagagcc
      481 agaaataagg caattacagc catacttaaa gaagattttg catatcttaa actaacatat
      541 atccctcaat acaatccgtt tatgcgaaca gggattgcta aactttggga aggaactcag
      601 tttggtaaaa atagtttttc gtctcgtgca gcattaagag atgttattat tcatgaagaa
      661 ttacatcata ggtggtggaa gcgaggattg aatgatcatc atcccaaagg tactgagatg
      721 gaacagaaat tttacgagac gattcgaaga tataagcgga tgagagggtg gttatagtgt
      781 ttggtgatga atttaaaacg actattgagt gtccttgctg tggaaaaata tgttctatag
      841 ctatagggca gacgagtcac aatggtatac tcgcatggta tgaatcaatt atatgccata
      901 attgcggatt acgtagtgaa tcagatgata taggctttcc cccagaaaaa attcgtctac
      961 aattgattga ttttgagggt ttatggaatg ttagacttga aaatataaaa tcacagatgg
     1021 aaacta
//
LOCUS       DAZKJR010000406         1020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKJR010000406 DAZKJR010000000
VERSION     DAZKJR010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.409"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..268)
                     /locus_tag="ACHOWS_005424"
     CDS             complement(<1..268)
                     /locus_tag="ACHOWS_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFQ0050825.1"
                     /translation="MRFICDNSYFLEGVKEYINPGKFIISGMQTDNVVIISSSRISEI
                     VGFLYKNNISDMNTLYFSSPEALRFLWGVVDSPVYDIRHLTERCS"
     gene            complement(392..868)
                     /locus_tag="ACHOWS_005425"
     CDS             complement(392..868)
                     /locus_tag="ACHOWS_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFQ0050826.1"
                     /translation="MQIIIMTRDRYLEYGLMRMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVIFHEYGCTFLADQLGISMKTVCAHLYNAMEKSGLRGVSVKYLCSTADR"
BASE COUNT          289 a          292 c          195 g          244 t
ORIGIN      
        1 ctgaacatcg ctcagtaaga tgtctgatat cataaaccgg actgtcaacc actccccaga
       61 gaaaacggag cgcttccgga gaagaaaaat aaagggtgtt catatcactt atattattct
      121 tatacagaaa gccgacaatt tcagatatcc gggaagaaga tattatcacc acattatctg
      181 tctgcatgcc gctgataatg aatttaccgg gattaatata ctccttcact ccctccagaa
      241 aataactgtt atcacaaata aaacgcatac ccgttccaca tgaaatttat cacgcctgac
      301 cacataacat taattatccg gagcaccggg atgcccgtga cacagcatgc acagaaattc
      361 ccgtcgcaac agccttcatc agaccggctt tttaccggtc tgcggtgctg cagagatatt
      421 taacactgac acctcgcaga ccgcttttct ccatcgcatt gtaaagatgc gcacataccg
      481 tcttcatgct tatccccagc tggtctgcca gaaaggtaca gccgtactca tggaaaataa
      541 ccccgaagac caccaccatc tcggtacggg tcagtggtct tgccgtatgt ccgaacagcc
      601 acgcaccacg acggatggcc tgcctgatat ctgtcaggca tctcacagag gaaacaggaa
      661 tgacaagaaa acgacgccca cagaacatac agtatgtaag cctttccaga ttacggtcac
      721 agagaatcac ataactgtct tcgggaagtg gcagacggcg ctttccggca tcaaacagtt
      781 cacggcctgt cgtgagcctg tatccgttca gcatacgcat caggccatat tccagatatc
      841 tgtcacgggt cataataata atctgcatac tctcagaccc ttatctcccg gataccggct
      901 ccgttcacca ttaccggtat aactgactca tcattcataa cggaaactgt ctgccgcata
      961 catcagtgcc cgctcttcca gctctgacag actcagctct ttcagttccc cttctccttc
//
LOCUS       DAZKJR010000407         1014 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKJR010000407 DAZKJR010000000
VERSION     DAZKJR010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.410"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..489)
                     /locus_tag="ACHOWS_005426"
     CDS             complement(31..489)
                     /locus_tag="ACHOWS_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050827.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPTQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILLR
                     GKETETISERLIREADTREYELWRPVRRFFSIIFLIVLLVCIHSLITTIK"
     gene            947..>1014
                     /locus_tag="ACHOWS_005427"
     CDS             947..>1014
                     /locus_tag="ACHOWS_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050828.1"
                     /translation="MQITEALISEPGEIRRFVQQAV"
BASE COUNT          302 a          168 c          196 g          348 t
ORIGIN      
        1 tattttcctg tatcagaaca gccataactt ttattttata gttgttatca gtgaatgaat
       61 gcaaactaat agaacaatga gaaaaataat ggagaaaaag cgacggaccg gacgccatag
      121 ttcatattct cgtgtatcag cctcacgtat caacctttct gaaatggttt cggtttcttt
      181 tcctcgcagc agaatatgtc tgtaatattt cctcatgaca tataccgaaa actgaaacca
      241 gattaatatg gatggacaca caacacataa acttctgatg atatcacccg ggaaagtatc
      301 ttgggtgggt aaaagaaagt catatactct ggataaaatc atcatcgaga taaatgagac
      361 gccagcacct atccagtatt cgatatgaga agatgctgca ttatatgcgt cagtaaaatt
      421 tttctttctt aatgaaggtg gaagtgctga aagttcagga atgcgttttc ttgtccagta
      481 tattttcata tagtaactca tataaaaagt gttctacttt actatgatga tgacgattta
      541 taaaaggctt ataatgacta cttctccttt cattctcgaa tttcacgata atgatagaga
      601 taatcattat caattgatag tgtcagtaat tttgacttac atcacacttt catggtttga
      661 tcaggtgatt gatttaatga aaagagtgtg tttgtggatg tattgtgtat atgtcttacc
      721 gggcatctgt tcttacatta attaatatat tgctatttaa gtattggtag tattgtcagt
      781 aacattcttt tggttaattt agttattatt cctcactgat taattgtcct tccgttctta
      841 ttgtttgtaa cttgtgtttg tatcgtacct ttcaggtgtt gatgcgcgct ttactgtgcc
      901 tgcaccggct tgacaaaatt caacaggggt tgaaaaggaa catttcgtgc aaataaccga
      961 agccttaatt tcagagccgg gggaaatccg gcgttttgtt cagcaggcgg tgga
//
LOCUS       DAZKJR010000408         1013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKJR010000408 DAZKJR010000000
VERSION     DAZKJR010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.411"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..1005
                     /gene="leuO"
                     /locus_tag="ACHOWS_005428"
     CDS             61..1005
                     /gene="leuO"
                     /locus_tag="ACHOWS_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFQ0050829.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          273 a          219 c          245 g          276 t
ORIGIN      
        1 ggattatttc tctgcattcc aataagggaa agggagttaa gtgtgacagt ggagttaagt
       61 atgccagagg ttcaaacaga tcatccagag acggcggagt taagcaagcc acagctacgc
      121 atggtcgatc tcaacttatt aaccgttttc gatgccgtga tgcaggagca aaacatcacc
      181 cgtgccgctc atgttctggg aatgtcgcaa cctgcggtca gtaacgctgt tgcacgcctg
      241 aaggtgatgt ttaatgacga gctttttgtt cgttatggcc gtggtattca accgactgct
      301 cgcgcatttc aactttttgg ctcagttcgt caggcattgc aactagtaca aaatgaattg
      361 cctggttcag gttttgaacc cgcgagcagt gaacgtgtat ttcatctttg tgtttgcagc
      421 ccgttagaca gcattctgac ctcgcagatt tataatcaca ttgagcagat tgcgccaaat
      481 atacatgtta tgttcaagtc ttcattaaat cagaacactg aacatcagct gcgttatcag
      541 gaaacggagt ttgtgattag ttatgaagac ttccatcgcc ctgaatttac cagtgtgcca
      601 ttatttaaag atgaaatggt gttggtagcc agcaaaaatc atccaacaat taaaggcccg
      661 ttactgaaac atgatgttta taacgaacaa catgctgcgg tttcgctcga tcgtttcgcg
      721 tcatttagtc aaacctggta tgacacggta gataagcaag ccagtatcgc gtatcagggc
      781 atggcaatga tgagcgtact tagcgtggtg tcgcaaacgc atctggtcgc tattgcgccg
      841 cgttggctgg ctgaagagtt cgctgaatcc ttagagttac aggtattacc gctgccgtta
      901 aaacaaaaca gcagaacctg ttatctctcc tggcatgaag ctgccgggcg cgataaaggc
      961 catcagtgga tggaagagca attagtctca atttgcaaac gctaactgaa tgc
//
LOCUS       DAZKJR010000409         1010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKJR010000409 DAZKJR010000000
VERSION     DAZKJR010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.412"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..558)
                     /locus_tag="ACHOWS_005429"
     CDS             complement(<1..558)
                     /locus_tag="ACHOWS_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFQ0050830.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVG"
     gene            complement(555..>1010)
                     /locus_tag="ACHOWS_005430"
     CDS             complement(555..>1010)
                     /locus_tag="ACHOWS_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFQ0050831.1"
                     /translation="SDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLARFQTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERLYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLTWSDEDGIVMVFDAQAEGEPS"
BASE COUNT          232 a          297 c          272 g          209 t
ORIGIN      
        1 ccccacctcc gccagcaggc gccggataaa ctcaaggtca gtttcccgcc accgggtgat
       61 gagttcccta ggtggatact cccgggagag ccggaattca aaatcctgcc cttccagacc
      121 atgtttacgt aatacctgct caaccacttc agtgactgac tgattgaggt agatttcgct
      181 ccctttcgta tagttcagaa gggcgatacg gggaacaatc gtcagtgcgt aacgggtctc
      241 atcattcgag gcaaagatac gacgaaacga ctcaaccacg ccgaataccg tcctgatcgg
      301 cagggccggt gttccgtcag acatcgggac ctggaaagta aatgacgccg gcttcagcag
      361 taacgcgtca caggcaatat ccgcagaaga gcaggtgaca accacgtcat agcgccaggg
      421 ctggctgagg gactcaaccg cgctgtaacg caacacatcc agaaaatggc tgcattcatg
      481 cacatgaacc cggtaccgtg attgccctga aagctgctgt acaacgctgc cagccttgct
      541 ggtcagggaa cggttcatga cggctctccc tctgcctgtg catcaaatac catcacaata
      601 ccgtcctcgt cactccatgt cagagtcagc gcctgtggtt tctgcttcgc ggccatatgg
      661 ctcagcaact gccggctcag taccggcagc agttgctggt tcagcagact ctccacattc
      721 cgggcgcccg tatccggcag caggcaggct gatgtcaggg catcgtacag acgttcatca
      781 accgatgtgc tcagcccata atggcagtgc aggcgttcgc agacctgtgc aagcttcatc
      841 tccacaatgg tgcgcatggc agactgcgtc agcggacggt aaatcacggt ctggaaacgg
      901 gcaagcagtg ctggctggaa gtggtcgcgc agcagtggac ggataagctc atgcagctcc
      961 gattccgttg tctccggctt ttcgctcagt tgctgcatca gaaggtcact
//
LOCUS       DAZKJR010000410         1000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKJR010000410 DAZKJR010000000
VERSION     DAZKJR010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.413"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1000
                     /locus_tag="ACHOWS_005431"
     CDS             <1..>1000
                     /locus_tag="ACHOWS_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFQ0050832.1"
                     /translation="QVTEAVLTQDGPVLENFTAGQSGTLSDDDRGGFIWLGGLAFGGG
                     LAAMALAAGGGGGSKHRHENHEADTTAPSAPELTAGDNGSVSVELPGDANKGDTVEIT
                     FEDENGDEQKVTLEKGDNGWTSSNPALIPDSQGDTATIAPDTVKDNSEVTAVAKDPAG
                     NESTPVTVTSKTDGVADAPVLTIPEVADSVANAAELKDGLQAEVKLPAGTVEGAVITL
                     TVTHPDQSTENVTHNVTGDEVTAGKVSMDIPEDAVVDGQNSVSVSLTQGSNPAKAGNK
                     VEFVVDGQVPGDTNGDGVADVTPAVAIPEATDGVNAKELKDGVQAEVTVPAGSAEGD"
BASE COUNT          267 a          206 c          316 g          211 t
ORIGIN      
        1 caggtaactg aagctgtcct gacccaggat ggccctgtac tggagaattt cactgcagga
       61 caatccggga ccttgtcaga tgatgatcgt ggcggtttta tatggttggg cggtctggca
      121 tttggtgggg ggcttgctgc tatggctttg gctgcaggtg gtggtggcgg cagcaagcac
      181 agacatgaaa accatgaggc agatacaaca gcaccgtcag caccggaatt aacggccgga
      241 gataatggtt cagtttctgt tgaactgccg ggtgatgcaa ataaaggcga tactgttgaa
      301 atcacctttg aagatgaaaa tggtgatgaa cagaaagtca cactggagaa aggggataac
      361 ggctggacat ccagcaatcc tgcactgatc ccggacagcc agggtgatac agccacaatc
      421 gccccagaca ctgttaaaga taacagtgaa gtcacagcag ttgctaaaga tccggctggt
      481 aatgaaagta ctccggtcac tgtgacgtca aaaactgatg gtgtggcaga tgctccggtc
      541 ctgaccattc cagaagtggc tgatagtgtg gccaatgcgg ctgagctgaa agatggttta
      601 caggcagaag tcaagttgcc ggcaggaaca gtggaaggtg cggtcattac actgacagtg
      661 actcatccgg atcaaagtac cgaaaatgtg acacataacg tcaccggaga tgaggttact
      721 gccggtaagg tatccatgga tattcctgag gatgctgttg ttgacggtca gaacagtgtg
      781 agtgtcagcc tgacccaggg cagtaatccg gctaaagcgg ggaataaagt ggagtttgtg
      841 gttgacgggc aggttccggg tgacaccaat ggtgatggtg tggcggatgt cacaccggca
      901 gtggccattc cggaagcgac ggacggtgtg aatgccaaag aactgaagga tggcgtacag
      961 gcagaagtga cggttccggc aggcagtgct gaaggtgata
//
LOCUS       DAZKJR010000411          996 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKJR010000411 DAZKJR010000000
VERSION     DAZKJR010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.414"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(231..902)
                     /gene="queE"
                     /locus_tag="ACHOWS_005432"
     CDS             complement(231..902)
                     /gene="queE"
                     /locus_tag="ACHOWS_005432"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFQ0050833.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          276 a          242 c          211 g          267 t
ORIGIN      
        1 attgattaaa aagctaaata tttaaaataa ctctatacaa actaaaatct accaaacttc
       61 gccgcaataa ttttcactcc agcgaaaaat taacgccaca gaatttgtag agaatgataa
      121 tgattagtcc gggatgggca agatggatga gttttcagag gcgacaaaca atataaatga
      181 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      241 tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      301 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      361 tcatcggtca gcgttgccag tagttcatcc agtgcttcaa tatcacgtac gcgccccact
      421 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacttcata gccgccgcgc
      481 atgttcagct ttggtgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta
      541 ccactggttt caatctggca gctaaaacca ttcttttcga gcagatcagt cagtggtagc
      601 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg
      661 cgaccaatga cagccagcaa gtcttcactg ctcgccgccc cccacttatc actctctttg
      721 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      781 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      841 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      901 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat
      961 gtcttattaa cattctgtta caggcaggtt taaaga
//
LOCUS       DAZKJR010000412          993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKJR010000412 DAZKJR010000000
VERSION     DAZKJR010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.415"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>993)
                     /locus_tag="ACHOWS_005433"
     CDS             complement(<1..>993)
                     /locus_tag="ACHOWS_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFQ0050834.1"
                     /translation="ILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESIT
                     PVTNTIPGEEDDDEPVFSEDAVSEHLRFRYGRRWQHKVRLLLVMGDPDVVRKAAPGLC
                     HDLWQEGDGYVLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALPTDGERD
                     ALLRYRQKADHQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVKGADEAFST
                     LALHLQKAGMAQILDEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMF
                     SPELTVAGAVPNTCPDSPAWKAVIDDCGAASGRKLGFSWLKMLRLILLSLIVLWG"
BASE COUNT          217 a          310 c          264 g          202 t
ORIGIN      
        1 cccccacagg acaatcaggg aaagcagaat caatcgcagc atcttcagcc agctgaagcc
       61 cagttttctg ccgctggcgg caccacagtc atcaatgacg gctttccagg ccggtgagtc
      121 ggggcatgtg tttggtaccg ctccggcaac ggtcagttca ggactgaaca tgacaccgcg
      181 taaacgccag gcagcagtcc cctgcatcag tccactgagc aaaacagcca ggctggcttt
      241 cagctcctgc cgcagtctga gtgacaactg aaggagaccg aaatcagcag gctcatccag
      301 tatctgcgcc attcccgctt tttgcaggtg taacgccagt gtggaaaacg cttcgtccgc
      361 acctttcaca gttccttccg gtccgaagat gacccctgtt gcggccgctt tcgcgtcctg
      421 ccgggcaccg gtagcgttgt cagtcagcca cagccagact ggtgcctgcc agcccaactg
      481 atggtcggct ttctggcggt aacgcaggag ggcatcccgt tcgccatccg tcgggagggc
      541 tgaaatgctc atcacctgaa tgatgccatc aaccggttgc ccggatcgca gacgcttcag
      601 ttttgaaagg aagatctcgt ccgggagtga ctgcgcgtct ccgccataaa tcagcacgta
      661 gccgtcgcct tcctgccaga ggtcatggca aaggcccggg gctgctttcc ggaccacatc
      721 cggatccccc atcaccagca ggagtcgcac cttatgctgc cagcgacgcc catagcgaaa
      781 gcgcaaatgt tctgaaacag catcttcgct gaaaaccggc tcatcgtcgt cttcttcacc
      841 gggaatggtg ttcgtcaccg gagtgatact ctcagccttt ttctgccccc actggaaata
      901 cagcccggat ttaccggcga agtcgaaagc cttctccgtc agccagccga gtaaaaacag
      961 cgtggcaacc agtatggcgc agaatgtgag tat
//
LOCUS       DAZKJR010000413          989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKJR010000413 DAZKJR010000000
VERSION     DAZKJR010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.416"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..417
                     /locus_tag="ACHOWS_005434"
     CDS             112..417
                     /locus_tag="ACHOWS_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050835.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            472..>989
                     /locus_tag="ACHOWS_005435"
     CDS             472..>989
                     /locus_tag="ACHOWS_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0050836.1"
                     /translation="MTSEVKLKTGNDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTASWYTIARMHTTGLSGLNEGLALIVALTRHHWPVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILTRHELKQSSKVALPE
                     AMVKGYITRLEN"
BASE COUNT          253 a          239 c          255 g          242 t
ORIGIN      
        1 tactgcgata tctgaagcaa cagcagtttg tggataatgt gatgactttc agggggtgat
       61 ttatggttcc ccttatccgt gtgcttaaca gacataaacg agggctgaat gatgaacccg
      121 ttatttaatg acatccagat gcgccttttt tatctgaatc atgcaccgta ttcctggcac
      181 tggaatgtca gattcaggcc acaggaagcc gtatatattg gtaatgatgc ctgccatatt
      241 accattacct gtaatcagtc cggttttcat ttaaccaggg acgggcagcg actgttcact
      301 gaacgttata tcagaacact cagtgagtta ttggcggttc tgaagcggcg gtgggatgtg
      361 acaccggcaa tcatccgtgc ggtggaatac ctttcccggg taccggtatc acactgacgc
      421 tctttccggg aatattaatt atgatttgat acagcaacac tgaatctgcc gatgacttct
      481 gaagtaaaac tgaaaacagg taatgacccc cggtcattac cggattatgc tgccctgcgc
      541 gatgaaatca gcaagctgac tcacccggca cgtccggatg tggactggcg ttatgtggag
      601 acgctctgcc tgcgtctgta tgaacacaat ggcgtggaat tacagacggc atcctggtac
      661 accatcgccc ggatgcatac aacagggtta agtggcctga atgaagggct ggctctgatt
      721 gtggcgctga cccggcatca ctggccagtg atgtggccgc tgaataccca cgcccggctg
      781 gaaattatta ccggtctgtt cagtcgtctg caaaaaacgc tccgggccat gccaccggat
      841 gagcgtgaca acctgcccct gctgtatcag acagaaacat gcctgaaaac gctgagtgac
      901 atactgaccc ggcatgaact taaacagagc agtaaagtgg ctttaccgga agccatggtg
      961 aagggatata tcacccgtct ggagaatca
//
LOCUS       DAZKJR010000414          986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKJR010000414 DAZKJR010000000
VERSION     DAZKJR010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.417"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..334)
                     /locus_tag="ACHOWS_005436"
     CDS             complement(<1..334)
                     /locus_tag="ACHOWS_005436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050837.1"
                     /translation="MTTCSELKERARFDGMHDASLEVAWQLLMTKSVSTITTEIISGA
                     DMSNFSVIIRERYKHAIAKSLLNHLSPDVVSDIIGLSHDEFNQQYKGNLTRQQHLRDI
                     AVSLTDKGF"
     gene            complement(418..>986)
                     /locus_tag="ACHOWS_005437"
     CDS             complement(418..>986)
                     /locus_tag="ACHOWS_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFQ0050838.1"
                     /translation="QTIRKIKSGFSKYLLNNSFNKFSLGYNVISGSIHPTKFTNADGN
                     HLHEVLSGDLGVLQDNTKAPLDSYLFEEVFYDSELERLNITDGEIHSVAVFTKIPKNS
                     IKIPVAGGYTYSPDFAYVVKTTKGDYLNFIIETKNVEGKESLRKEELDKIKHAQKLFE
                     QVSKSVKVTFITQFSGDRIHDLIKNVIQ"
BASE COUNT          289 a          174 c          173 g          350 t
ORIGIN      
        1 tgaatccttt atctgttaaa cttacagcaa tatctctcag gtgttgttgt ctggttaaat
       61 tacctttata ttgttgattg aactcatcat gagataaacc gatgatgtca gaaacaacat
      121 cgggagataa atgattgaga agagattttg ctattgcatg tttatatcgt tccctgataa
      181 tcactgaaaa attggacatg tctgcaccgg atattatctc agtagtgata gtggatacac
      241 ttttcgtcat aagtaattgc catgccactt caagagaagc atcatgcata ccatcaaaac
      301 gggctctttc cttcagttca gaacatgttg tcattgaact cacctgcgaa ttttgtgatg
      361 tgtgtataat tataaagtgg gggtgaaacc acctccactt tgttatcatg catgtgttta
      421 ctgaatcaca ttttttatca gatcatggat tctgtcacct gagaattgag ttatgaaagt
      481 tacttttaca gatttactta cctgttcgaa aagtttttgt gcatgcttta ttttatccag
      541 ttcttcttta cgtaaacttt ctttaccttc gacatttttt gtttcaataa taaagttaag
      601 gtagtcaccc tttgttgttt ttacaacata agcaaagtca ggagaatatg tgtatccacc
      661 tgccaccgga attttaattg agtttttcgg tatctttgta aaaacagcaa cagagtggat
      721 ctctccgtct gttatattga gtctttcaag ctctgaatca taaaaaactt cttcaaaaag
      781 ataagagtcc aggggggctt ttgtattatc ctgtaatact ccaagatcac ctgataatac
      841 ctcatgcagg tgattaccgt ctgcattagt gaattttgta ggatgaattg agcctgaaat
      901 cacattatat ccaaggctga atttgttaaa tgaattgttc aacaaatact tgctaaaccc
      961 agatttaatc tttctgattg tctgaa
//
LOCUS       DAZKJR010000415          979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKJR010000415 DAZKJR010000000
VERSION     DAZKJR010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.418"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(705..780)
                     /locus_tag="ACHOWS_005438"
     tRNA            complement(705..780)
                     /locus_tag="ACHOWS_005438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(745..747),aa:Ile,seq:cat)
BASE COUNT          301 a          206 c          178 g          294 t
ORIGIN      
        1 tgtcattata aaaaaccgct catttgagac aattgctgac attaactgtt tcacttgcta
       61 cgcatggact ttttaattaa attagcacaa gaatgttaaa tttaataaac aaaaagttat
      121 ttcgctgtat gataaaaacc aaccgttatg atttattagt ggaattcgtt tttcgagtgt
      181 tagaaattta tatctaaata gcgttaatta atgagcaaag ccagactcct gtaacgctca
      241 taagactcat ctgcctgcgg cgggtgttct ggtcagtagt agatatttaa ggcgtggcag
      301 agacatttca tccttactct acggcattgg tccacataca aatcgtggtt gtgacacttc
      361 acttatcatc agtgagcgaa cagagaatag ttcagtgatt tgagtaatta acctgattaa
      421 atgaagaggc atgataaatg ataatactct ggctttatcg ttaattactt aattccacat
      481 gtaagcaatt tgcccgcttg gcatagcggg cattttttcc aggtactttt gaatgagtac
      541 tgatggataa atacattgca gtggcgtgcc acgtaccaaa acaccagccc tcattcgaaa
      601 ccacccaccg cacttcttcc tttaaatggc gttagtcatg aaatatagac cgccatcgag
      661 taccccttgt acccttaact cttcttgata catgaatgat gatttggtgg cccctgctgg
      721 acttgaacca gcgaccgggc gattatgagt cgcctgctct aaccactgaa ctaaagggcc
      781 ttaagtatgc aataacaata cttataaacc acgcaataaa catgatgatc ttatgatgta
      841 aataacagat ttttatgcgt tctcatccat cctgggtcgt caatacatgc gacataaatc
      901 ccgacaccgc ttccattcgc aaagtcgata ctcgcagtca acaagcatgt taataataag
      961 cactatctat agttatcat
//
LOCUS       DAZKJR010000416          953 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKJR010000416 DAZKJR010000000
VERSION     DAZKJR010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.419"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..546
                     /locus_tag="ACHOWS_005439"
     CDS             <1..546
                     /locus_tag="ACHOWS_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFQ0050839.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLSSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGANLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..>953
                     /locus_tag="ACHOWS_005440"
     CDS             562..>953
                     /locus_tag="ACHOWS_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050840.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPLPFKGEQAIRCMADGEVIAYRICRDYLT
                     LGWESGPLSFSGSFVLVKHYIQPGEKET"
BASE COUNT          250 a          217 c          266 g          220 t
ORIGIN      
        1 attaggtttg taaccgataa aatcagggaa cttcagtctg cggtcctgct gggtagcgcg
       61 ccacaggggg tggccctgac ctcaggtgaa caccttcagc tcagcagtac ccgtaatacc
      121 atgatcaatg caggtcagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caatcaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tttcagaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaaccctgac gctgaacggt
      361 ggcgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg caaatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt gggaggagaa
      661 tctaccgggc tgtacatgat aggccgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccgcttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgcta ggctgggaat caggtccgct aagtttttcg
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac cag
//
LOCUS       DAZKJR010000417          953 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKJR010000417 DAZKJR010000000
VERSION     DAZKJR010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.420"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..229)
                     /locus_tag="ACHOWS_005441"
     CDS             complement(<1..229)
                     /locus_tag="ACHOWS_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFQ0050841.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDTN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCS"
     gene            complement(325..801)
                     /locus_tag="ACHOWS_005442"
     CDS             complement(325..801)
                     /locus_tag="ACHOWS_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFQ0050842.1"
                     /translation="MQIIIMTRDRYLEYGLMCMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
BASE COUNT          273 a          265 c          180 g          235 t
ORIGIN      
        1 ctgaacatcg ctcagtaaga tgtctgatat cataaaccgg actgtcaacc actccccaga
       61 gaaaacggag cgcttccgga gaagaaaaat aaagggtgtt cgtatcactt atattattct
      121 tatacagaaa accgacaatt tcagatatcc gggaagaaga tattatcacc acattatctg
      181 tctgcatgcc gctgataatg aatttaccgg gattaatata ctccttcatt ccctccagaa
      241 aataactgtt atcacaaata aaatgcatac ccgttccaca tgaaatttat cacgcctggc
      301 cacataacat taattatccc ggatttaccg gtctgcggtg ctgcagagat atttaatact
      361 gacgccgcgc agaccatttt tctccatcgc attgtaaaga tgcgcacata ccgtcttcat
      421 gcttatcccc agctggtctg ccagaaaggt aaaaccgtac tcatggaaaa caactccgaa
      481 gaccaccacc atctcggtac gggtcagtgg tcttgccgta tgtccgaaca gccacgcccc
      541 acgacggatg gcctgcctga tatctgtcag gcatctcaca gaggaaacag gaatgacaag
      601 aaaacgacgc ccacagaaca tacagtatgt aagcctttcc agattacggt cacagagaat
      661 cacataactg tcttcgggaa gtggcagacg gcgctttccg gcatcaaaca gttcacggcc
      721 tgtcgtgagc ctgtatccgt tcagcataca catcaggcca tattccagat atctgtcacg
      781 ggtcataata ataatctgca tactctcaga cccttatctc ccggataccg gctccgttta
      841 ccattaccgg tataactgac tcatcattca taacggaaac tgtctgccgc atacatcagt
      901 gcccgctcct ccagctctga cagactcagc tctttcaggt ccccttctcc ttc
//
LOCUS       DAZKJR010000418          953 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKJR010000418 DAZKJR010000000
VERSION     DAZKJR010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.421"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..428
                     /locus_tag="ACHOWS_005443"
     CDS             <1..428
                     /locus_tag="ACHOWS_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0050843.1"
                     /translation="NTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDAIYHSHD
                     TIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIVLAATDS
                     TSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            444..731
                     /locus_tag="ACHOWS_005444"
     CDS             444..731
                     /locus_tag="ACHOWS_005444"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0050844.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TTSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            744..>953
                     /locus_tag="ACHOWS_005445"
     CDS             744..>953
                     /locus_tag="ACHOWS_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0050845.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQ"
BASE COUNT          246 a          206 c          256 g          245 t
ORIGIN      
        1 taaatacaga ggtcgttcag gtcgtggaac aggtcaaaga ctggcgggaa gccgtggcga
       61 tttcatgccg accgttgatt gaaaacggct ctatcgaacc gcgttatgtt gatgcgatct
      121 atcactcgca cgacactatc ggtccgtact atgtggttgg ccctggtatt gcgatgcctc
      181 atgcgcgccc ggaagagggg gctaacaagc tgtcattagc actgaccctg atcccttcag
      241 gtgtgaattt tgatgccgat gaaaacgatc cagtgaaatt actgattgtg ctggctgcaa
      301 ccgacagtac cagccacatt gaagcgattt ctcaactcgc caagttattc gataacgaaa
      361 aagacataca ggccattttg acggccaaaa cgacgcagga cattttgtct gttattgcgc
      421 gctattaatc cagaggaaca tttatgaaaa ttacagtggt ttgcggtaac ggcttaggca
      481 ccagcctgat gatggaaatg agcattaaaa gcattttgaa ggatttgtcc gtcagcgctg
      541 atgttgatca tgtcgacctg ggctccgcga aagggacgac gagtgatatc tatgtcggca
      601 ccaaagacat tgccgagcag ttagccgcgc agtctgtcgc aggcaaaatc gtcgcactgg
      661 aaaatatgat tgataagaaa gccatgagag aacgtttgtc cgtggtatta accgaattag
      721 gcgcactgta agctgggggc gatatggatt tctttcgttt tttgatgagc gatgtacttt
      781 ccgaacccgc agtgctggtc ggtttaatcg ccctgattgg cctgattgca cagaaaaaac
      841 cggtaaccga atgcattaaa ggtaccgtca agaccattat ggggtttgtg attttaggcg
      901 caggggcggg tttagttgtg tcgtcactgg gtgacttcgc aaacattttc cag
//
LOCUS       DAZKJR010000419          947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKJR010000419 DAZKJR010000000
VERSION     DAZKJR010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.422"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..586)
                     /locus_tag="ACHOWS_005446"
     CDS             complement(<1..586)
                     /locus_tag="ACHOWS_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFQ0050846.1"
                     /translation="MNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTT
                     YRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAASQLVTQRAGF
                     FPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASG
                     TGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETD"
     gene            complement(617..778)
                     /locus_tag="ACHOWS_005447"
                     /pseudo
     CDS             complement(617..778)
                     /locus_tag="ACHOWS_005447"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT          164 a          293 c          256 g          234 t
ORIGIN      
        1 ggtcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccacaac
       61 tgtcatagcc ataggactca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctgaccgttc cggtccagcc ggtattcccg ccgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacagcc gacacattgc ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgaggggag cagcccgccc catgtggttt cctccgggaa aaatccggcg cgctgggtga
      301 caagctggct ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcaccgg
      481 gatgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtagtggta ttcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgac ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca gaccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggctactc cgttgtaccc agctcataga atttatctag
      841 caatttgtat taagggggtt tcacccacat gttttaataa actcattaac cagatggcct
      901 cttctctcgt tccggagtat attaccttcg attcatttct aataatt
//
LOCUS       DAZKJR010000420          944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKJR010000420 DAZKJR010000000
VERSION     DAZKJR010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.423"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>944
                     /locus_tag="ACHOWS_005448"
     CDS             <1..>944
                     /locus_tag="ACHOWS_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFQ0050847.1"
                     /translation="VAGALPEIPEPFKYGVGGIDNGKIYIGLGSLGNNWYMIDTNQSE
                     KKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVITLQKDVYSYDIAKDKWEK
                     LMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVELSKGNSALTEKVNRDYFS
                     KPVDDYFLNNHMISYDPSKNQWKNLGTTPFPGTAGSSVIFAEQQIYILGGERKPGLRS
                     VRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGE
                     YWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVS"
BASE COUNT          298 a          153 c          230 g          263 t
ORIGIN      
        1 ggtagccgga gctttaccgg aaattcctga accatttaaa tatggtgtag gagggatcga
       61 taatggtaaa atatatatag gactgggaag tctgggaaat aactggtata tgatagatac
      121 aaaccagagt gaaaaaaaat ggactaagat agcacaatgg ccgacagttc ccagagagca
      181 ggcaacagct actattattg atggtaagat ctatgtattt ggaggaattg gaaaagataa
      241 aagtggagtc ataaccttac aaaaagatgt ctattcctat gatattgcta aagataaatg
      301 ggaaaaattg atgacccgtc cccctgtatc tcttgccggt catgtttctt ttatccataa
      361 tggtcatgcg gtatctactg gtggtgtaaa tgagaatatt tttaatggat acttttcaga
      421 tgtcgagtta tcaaaaggga attccgcgct gacagagaaa gtcaacaggg attattttag
      481 taaacctgtt gatgattatt ttttaaataa tcatatgatt tcttatgacc cgtcaaaaaa
      541 ccagtggaag aacctgggaa cgactccatt tccgggaact gctggttcat cggtgatttt
      601 tgcagaacag caaatttata tactgggagg ggagagaaaa ccgggccttc gttctgttag
      661 atcctggacg ggggagcttt cgcatgatcg aattaaatgg tctgaacttc caccagtagc
      721 ttcacccgag ggggtgtcag gagcatatgc ctctgttatt gatggaaata tctttctggc
      781 cggtggtgca tatttcccag gggcggcaga aaagtatagc aatggtgaat actggtctca
      841 taaaggactg gataaagcct attcaaaaga gatatatcag ctaataaaaa atgactggaa
      901 aaaagttggt tctttgccgg aaggacttgc ttatggtgta agcc
//
LOCUS       DAZKJR010000421          942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKJR010000421 DAZKJR010000000
VERSION     DAZKJR010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.424"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>942
                     /locus_tag="ACHOWS_005449"
     CDS             <1..>942
                     /locus_tag="ACHOWS_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFQ0050848.1"
                     /translation="PAPVLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRTIAA
                     TTWSEYRQYLERDAALERRFQMVRVDEPDDETACLMLRSLKSRYAEHHNVHITDEAVR
                     AAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQLTRIRSQLASLGMEKQAL
                     LEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARECELTGELLESRSDISRQSE
                     TLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGVPLSSLMKDEQTELLHLEKDIG
                     RRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLVG"
BASE COUNT          197 a          256 c          295 g          194 t
ORIGIN      
        1 accggctcct gtactgttgt ttatagatga agcccatacc cttatcgggg ccggtaatca
       61 ggccggcggc gcggatgcgg ccaacctgct gaagcctgcg ctggcgcggg gtgaactgcg
      121 taccattgcc gccaccacct ggtctgagta cagacaatac ctggaacgtg acgcggctct
      181 ggagcggcgt tttcagatgg tcagagtgga cgagccggat gatgagacgg cctgtctgat
      241 gctgcgctcc ctgaaatccc gttatgcgga acatcataat gtgcatatca ccgatgaggc
      301 ggtacgtgct gccgtcacgc tgtcgcgccg ttatctgaca gaacgccagt taccggacaa
      361 ggccgttgac ctgctcgata ctgctgctgc ccgtgtgcgt atgagcctcg acacggtgcc
      421 ggaacagctg acccgtatcc gttcacagct cgcttccctc ggtatggaaa aacaggcact
      481 gctggaagat attgccgttg gtcatcagaa tcacggcgaa cgtctgtctg ccattgagca
      541 ggaagagaat gtgctgatga cagcacgtga tgacctggag cagcagtacg cccgggagtg
      601 cgaactcacc ggtgagttac ttgaaagtcg cagcgatatt tcccgtcaga gtgagacact
      661 gcatctgcag caggcactgc acgacattca gcagaatcag cccctgctca gtgtggatgt
      721 tgacgtacgc accgttgccg gtgtggtcgc ggactggacc ggggtgccgt tatcttccct
      781 gatgaaggac gaacagacgg aactgctgca tctggaaaag gatatcggca gacgggtggt
      841 cggacaggac gtggcactgg aatccattgc gcagcgtctg cgtgcggcga aaacgggtct
      901 gacatccggt aacggccccc agggggtgtt cctgctggtt gg
//
LOCUS       DAZKJR010000422          938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKJR010000422 DAZKJR010000000
VERSION     DAZKJR010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.425"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            131..319
                     /locus_tag="ACHOWS_005450"
     CDS             131..319
                     /locus_tag="ACHOWS_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFQ0050849.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
BASE COUNT          230 a          155 c          238 g          315 t
ORIGIN      
        1 atgaagttga gttgttgtac aaggggatgc ttctggtcag aaaagggagg ttgtgaagca
       61 ggacagctta tttccggtaa tgttaaacgc agcctctgat tcgttaattt cataatcata
      121 aggagttttt atgcgtctgt tgattgcttt tcttcttccc tggctgactt ttttcaccat
      181 cggccggcct ctgacgggga ttttttgtct ggtgttacag ataacgctgc tgggctggat
      241 accggcaaca atatgggccg tgtatgcgct ttcgcagtat aagacggaac agaaaatccg
      301 cgaagccacg gggaaatgat gactgcagaa gagcgggttg tcatagctgt gtatatgatg
      361 cgggataaag gcccggagat tcctgaaaat aaaatggttc gtggaatata aaacagggaa
      421 aaaccggatg tgatggacgc ttctggcgag gcggtttcat ggattgtccg taatcaggat
      481 atgctgcccg gttacgtcgt gaatcttgtg gcagcagtgt gtttttactt ttgaaacgta
      541 tatgacagtt ttgtgtgccg gttcagaaca aggagtgatt tgtgataact tgtgttttta
      601 tttttactgc tatccttctg gcagtcataa cttttatccc tgtttccggt aatacatcat
      661 gggttgtcag ggtatgggat tttccccgac tacagatact tttgctgtgt actttgaatg
      721 tttttctgtc tgttgttttt cttgactcat ttgcagagtt gtttgttgtg ataataagtt
      781 taatttgtgt cgcttaccag ttggtatgga taattcctta tacagtaatt ggcaagaagc
      841 aggtaaagta aataaaggac tctgatggat ttccgggttt aagtatactg gtattcaatg
      901 tccttatgac caataaaaaa tatatgcctt tgcttgaa
//
LOCUS       DAZKJR010000423          932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKJR010000423 DAZKJR010000000
VERSION     DAZKJR010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.426"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..>932)
                     /locus_tag="ACHOWS_005451"
     CDS             complement(246..>932)
                     /locus_tag="ACHOWS_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0050850.1"
                     /translation="LWWEQKLSPLDEVFPYSLKCPKENLYGMHVYSRMILSDAEIQYL
                     VDKEIPSMHMIVHMPEGQRIRLHCLHPVPPSPTESDESTDRDSELLMVGKSVADSELP
                     VILAGDLNDVAWSATTRLFMKISGLLDPRRGRGFFSTFHSHYFFLRWPLDHVFHSQHF
                     LLKSLRRLPDMGSDHFPVMAELVYAPAHGMQQESLEKEKSYEVLAQKKMDMTDSTAED
                     VHTPGGQKKS"
BASE COUNT          262 a          217 c          184 g          269 t
ORIGIN      
        1 atttttgacg gtaaagatac aaggggaagt atgtctgtaa ggagtaccct gaataatgga
       61 caggcatgca tcgtggctgt tcccctgaaa ttatcagtac aaccagtcag ctcataatcc
      121 ctgtctcttt ttccggggag ccctcagggg gactttccct tatcccccca gtttctggag
      181 agacgtaaaa aagaattaat catatgtcag gtatctgaat atgtcccgta attaatatat
      241 ttacgtcagg attttttctg cccgccagga gtatgaacat cttccgccgt tgagtctgtc
      301 atatccattt ttttctgtgc cagtacttca tatgattttt ctttttccag actctcctgc
      361 tgcatcccgt gagccggagc atatacaagc tcagccatca ctggaaaatg atctgaaccc
      421 atatccggta aacggcgtaa acttttaagc agaaaatgct ggctgtgaaa tacatggtca
      481 agcggccatc gcaggaaaaa ataatggctg tgaaacgtgc taaaaaaacc acgtccccgg
      541 cgtggatcca gcagtccact tattttcatg aataaacgag tggtcgcaga ccacgcaaca
      601 tcattcagat cccccgctaa tattacaggt aattcagaat ctgcaacact tttcccgacc
      661 attaaaagtt ctgagtcacg atcagttgac tcatcacttt cagtcgggct cggtggaaca
      721 ggatgaagac aatgtaatct tattctctga ccttccggca tatgaactat catatgcatg
      781 gagggaattt ccttgtccac taaatactgt atttctgcat cacttaaaat catccttgaa
      841 tatacatgca tgccatataa attttctttc gggcatttca gggagtatgg aaacacttca
      901 tccagaggtg atagtttctg ttcccaccat aa
//
LOCUS       DAZKJR010000424          922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKJR010000424 DAZKJR010000000
VERSION     DAZKJR010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.427"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..888
                     /locus_tag="ACHOWS_005452"
     CDS             88..888
                     /locus_tag="ACHOWS_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050851.1"
                     /translation="MNSKKVCCICVLFSLLAGCASQSSIDEKKKKAQVTQSNINKNIP
                     PQLTDKDLFGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YHDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKMSNKELSNDHVTEVQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          288 a          167 c          213 g          254 t
ORIGIN      
        1 gttattattt aagtggggta tataattaga gtccgttatc agtgctaaat attctaatca
       61 ttatgacagg cgagggagtg tccgattatg aattcaaaaa aggtttgttg catatgtgta
      121 ttattctcgc tgcttgcagg atgtgcctct caatcttcta ttgatgaaaa aaagaaaaaa
      181 gctcaagtta cacaaagtaa tattaataaa aacattcccc cgcaactgac agacaaagat
      241 ttattcggta atgaaaccac tctggctgta tccgaggagg atattcaggc tgcgcttgaa
      301 ggagatgagt ttcgtgtgcc tctcaattct cccgtaatac ttgtccagtc tggcagtcgc
      361 gcaccggaaa ccattatgca ggaggagatg agaaaatatt atactgtttc cacattctct
      421 ggtatcccgg acaggcaaaa gcctctgact tgtaacaaaa ataaagataa aaatgagaac
      481 gaggatgttg ccagtgctga aaatatgaac tggatgcagg cattgcgttt tgtggctgcg
      541 aaagggcatc agaaagcgat tattgtttac catgatacgt tgcagacagg aaaatatgac
      601 tctgcgctga aatcaacagt atggtccgac tataaaaatg aaaaactcac tgacgctatc
      661 tccctgcgct acctggtacg tttcacgctg gtggatgtgg caacaggtga gtgggctacc
      721 tggtcgccgg tgaattatga atcgagagta atttttccgc cggctggaat aacaaaaatg
      781 agcaataaag aactatcaaa tgaccatgtt acggaagtgc aactgtttaa tttgaaacaa
      841 aaaacatatg tatcgatggt gaaagacttg gtgagtcgat tccagtaatc gttattgtta
      901 tgctgccgcc tgtagtttag gg
//
LOCUS       DAZKJR010000425          893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKJR010000425 DAZKJR010000000
VERSION     DAZKJR010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.428"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>893
                     /locus_tag="ACHOWS_005453"
     CDS             <1..>893
                     /locus_tag="ACHOWS_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0050852.1"
                     /translation="LLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVASLQGEEHIRSV
                     HLLGALTENPHLVRCDGVWPLLTLSQSQLQRLSPLLDAQSDECPERLQDAEPVLPQGD
                     NVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRYITASAREGKIDPVSGRDTEIRQM
                     VDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLRTLDLGLLQAG
                     AGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAANLLKPALARG
                     ELRTIAATTWSEYRQYLERDAA"
BASE COUNT          178 a          257 c          284 g          174 t
ORIGIN      
        1 ctgctcggct ggctggataa ccagccccgt agcgtacgca gccgcccgca gcttgcgccg
       61 tccctgaatg ccctgctgaa acaggcctgg atggtggcct cattacaggg agaagaacat
      121 atccgcagcg tgcatctgct gggtgccctg acggaaaatc cgcacctggt tcgctgtgac
      181 ggggtgtggc ccctgctgac actgagtcag agccagttgc agcgtctgtc cccgctgctg
      241 gatgcgcagt ctgatgagtg tccggagaga ttacaggatg cagagcctgt gctgcctcag
      301 ggagataatg tgacctttat cgggcgtccc gtcggtgcgg atacggcggg tataccgtca
      361 ggtgacctgc cgcctgtgtt acagagtgcg ctggataaat tcacccggta catcacggcc
      421 agcgcgagag aggggaaaat tgacccggta tcgggacgcg atacggaaat ccgtcagatg
      481 gtggatattc tctcccgccg ccgcaagaac aacccgattc tggtggggga ccctggtgtg
      541 gggaaaacgg ctctggtgga agggctcgcc ctgcgtattg cggaaggtaa cgtaccggaa
      601 tcactcagac ccgtcaccct gcgcaccctt gacctcggcc tgctgcaggc cggtgccggc
      661 gtgaaaggtg aatttgaaca gcgcctgaaa aacgtgattg atgccgtgca gcagtcaccg
      721 gctcctgtac tgctgtttat agatgaagcc cataccctta tcggtgccgg taatcaggcc
      781 ggtggcgcgg atgcggccaa cctgctgaag cctgcgctgg cgcggggtga actgcgcacc
      841 attgccgcca ccacctggtc tgagtacaga caatacctgg aacgtgacgc agc
//
LOCUS       DAZKJR010000426          878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKJR010000426 DAZKJR010000000
VERSION     DAZKJR010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.429"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>878
                     /locus_tag="ACHOWS_005454"
     rRNA            <1..>878
                     /locus_tag="ACHOWS_005454"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          205 a          198 c          279 g          196 t
ORIGIN      
        1 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
       61 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
      121 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
      181 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg
      241 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg
      301 ttctaacgtt ggcccctgat cggggttgcg gacagtgtct ggtgggtagt ttgactgggg
      361 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc
      421 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg
      481 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa
      541 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt
      601 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg
      661 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct
      721 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg
      781 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga
      841 agagataagt gctgaaagca tctaagcacg aaacttgc
//
LOCUS       DAZKJR010000427          878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKJR010000427 DAZKJR010000000
VERSION     DAZKJR010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.430"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            290..406
                     /locus_tag="ACHOWS_005455"
                     /pseudo
     CDS             290..406
                     /locus_tag="ACHOWS_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(511..587)
                     /locus_tag="ACHOWS_005456"
     tRNA            complement(511..587)
                     /locus_tag="ACHOWS_005456"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Arg,seq:tct)
     gene            complement(601..677)
                     /locus_tag="ACHOWS_005457"
     tRNA            complement(601..677)
                     /locus_tag="ACHOWS_005457"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(641..643),aa:Arg,seq:tcg)
     gene            complement(687..762)
                     /locus_tag="ACHOWS_005458"
     tRNA            complement(687..762)
                     /locus_tag="ACHOWS_005458"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(727..729),aa:Ile,seq:cat)
BASE COUNT          216 a          231 c          225 g          206 t
ORIGIN      
        1 tcgtagtgtt taaatgccat gaatcctccc ggtagggata atgttgtgag tcacataagg
       61 gacgggctga agtccggaag ttacaggaca atggcagaag ggagactaca gcccgcaata
      121 cgaaaaaggc cgcgctattg cgcagagtga ttactgtcgg gtattattcg ccagctgaaa
      181 tattacttca cgttttgttg gtttattcct tgctgcccgc gtctcccggc gcgggctttt
      241 tttgtccata agaaagcccc tccggagagg ggctggagag tggcgctatg tgccattgca
      301 tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga ttatcccata
      361 tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt tcttttaaca
      421 aactccccgc aaaacagaca actgtcaacc gtctgaattg tgagacattt aaaaaaaagg
      481 cccgcaaaag cgagccaggg aaaataagtg tggcgcgttg tactggattc gaaccagtga
      541 ccgattgctt agaaggcaat tgctctgtcc ggctgagcta acaacgcatg atgcagataa
      601 tggaccgcca tcggggactt gaaccccgcg cagccagctt cgaaggctgg cgctctgtcc
      661 cgatgagcta atggcgatat ctgatatggt ggcccttgct ggatttgaac cagcgacctg
      721 gcgattatga gtcgctcgct ctcaccactg agctaaaggg ccgggcgcag gataataacg
      781 gtacgtaact aattctgcaa tatcatccgt tctgactaac taaattctga acttccctga
      841 ccgtctgctc aaaacgcccg ctctccagct caacgcca
//
LOCUS       DAZKJR010000428          877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKJR010000428 DAZKJR010000000
VERSION     DAZKJR010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.431"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>877
                     /locus_tag="ACHOWS_005459"
     CDS             <1..>877
                     /locus_tag="ACHOWS_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0050853.1"
                     /translation="VPNNEAIVSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFL
                     TGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDV
                     AIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTC
                     LFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLV
                     PNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGA
                     AAGVFGNATGGRRGA"
BASE COUNT          182 a          194 c          235 g          266 t
ORIGIN      
        1 tagtgccgaa caatgaagcc attgtttccg tagcgcagaa aagcttcggg aaagaaatgg
       61 cgatgattat gttctttgcg atggttatca acattatgat tgcccgtttc acaccgtgga
      121 aatttatctt cctgaccggt catcacacgt tgtttatgtc gatgatggta gcggtaattc
      181 tggcaacagc aggcatgacc ggcattacgc ttatagccgt tggctctttg gttgttggcg
      241 tggcaatggt ctttttcccg gctattgcgc atccgtacat gaagaaagtg accggttctg
      301 atgacgttgc catcggccac ttctcaacat tatcttatgt attggccggt ttcatcggca
      361 gcaagtttgg taataaagag cactcgactg aagacatgaa tgtgccgaaa agtctgctgt
      421 tcttgcgcga cacgccggtt gctatctctt ttaccatgag cataattttc ctggtgacgt
      481 gcctgtttgc gggtgcagat gcggtgaaag agcttagcgg tggtaaaaac tggttcatgt
      541 tctctatcat gcaatccatt accttcgcag ctggcgtgta catcattctg cagggtgtgc
      601 gcatggtgat tgcggagatt gtcccggcat tcaaaggtat ttcagacaag ctggttccga
      661 atgccagacc tgctctcgat tgcccggtcg tcttccctta cgcacctaac gctgtactgg
      721 ttggtttcct gagtagcttt gctgcaggtt taatcggtat gttcacgttg tatctgctga
      781 acatgatcgt gattattcct ggtgtggtgc cgcacttctt cgtaggtgca gctgcagggg
      841 tattcggtaa tgcaacgggt ggacgtcgtg gtgcgat
//
LOCUS       DAZKJR010000429          870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKJR010000429 DAZKJR010000000
VERSION     DAZKJR010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.432"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>870
                     /locus_tag="ACHOWS_005460"
     CDS             <1..>870
                     /locus_tag="ACHOWS_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFQ0050854.1"
                     /translation="SLCDVFTWHKLCHQARNKLSLSPESPAGWFESGCCEDLLNALNK
                     NKLRDYDALIIDEAQALHSSWLETLSCWFENKRIIAFCDETQTFSFEKGTNYADLQRI
                     INPQFSFNLSVVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTLLN
                     EILQLKESSVLPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITLYS
                     ESMEDAQLFCAYSRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAINEAKHNSS
                     SSVLMAGFFREGE"
BASE COUNT          267 a          155 c          171 g          277 t
ORIGIN      
        1 agtcattatg cgacgttttt acctggcaca aactttgtca tcaggctcga aacaaattat
       61 ccctgtcccc cgagtctccg gcaggctggt ttgaatcagg atgctgcgaa gatttgctta
      121 atgcattaaa taaaaataaa ctaagagatt atgatgcttt aatcattgat gaagcacagg
      181 cgttacattc ttcgtggctg gagactttat cttgttggtt cgaaaataaa aggattatcg
      241 cattttgtga tgaaactcaa acattttcct ttgaaaaagg aacaaattac gctgatcttc
      301 agcgaataat aaatccccaa ttttctttta acttatctgt tgtgttaaga tgtcctagag
      361 ccgttactga ttatttaata gctgtcagcc ctgcctcata ccaaattact tcccctagga
      421 ataacgaacc ggatacgcta aaagaattag ttgttacaga tccactctcc acattgctta
      481 atgaaatttt acaattgaaa gaaagttctg tactgcctga tgagattact gtccttgttc
      541 ctacggattt agtgaaaaat tccctccaga atgatatgag aactttgggg gtgaatattg
      601 aaacagtatc tcgttttcga ggtatggaat catctgtcgt gattacattg tattccgaaa
      661 gcatggagga tgcacagtta ttttgtgctt attcaagagc tacaactgct tttattgcaa
      721 tttatgattc ggagaaatta gcatggagag aaaaaaataa atttttgcat aaactggtta
      781 acagaaagga ggttcagaga gcaattaatg aagcaaagca taattcttcc tccagtgttc
      841 ttatggccgg ttttttccgc gaaggagagt
//
LOCUS       DAZKJR010000430          868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKJR010000430 DAZKJR010000000
VERSION     DAZKJR010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.433"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>868
                     /locus_tag="ACHOWS_005461"
     CDS             <1..>868
                     /locus_tag="ACHOWS_005461"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0050855.1"
                     /translation="ICFTGFDQSDKSRLSALAKENGIDVKSDVGKSLFFLCVGKNKGW
                     RKVQKASDYGVIILTEEQFINFIDTGEIPLEQDCVVENDISKSLEKDVNTYKSMIDEF
                     SLSIRTVREFRRSNALIAVFVDGYAAGWRFAVKECYKDSLDIKLTPVKTEFFNYEIWT
                     QGDSFQFNRGDVFYSDRLAYDSYCDFLKLDDAVVLQVKYECFSGYDSVSTFDGVMDGE
                     FSFSRMEPRPVYKLPVLIETQLYDPGKIIVDVFVPDAAKKQLKLKDTLELKQDEFVCL
                     LQSGCCWVKRREA"
BASE COUNT          236 a          123 c          208 g          301 t
ORIGIN      
        1 caatttgttt taccggattt gaccagtctg ataaatctcg cttaagtgct cttgcaaaag
       61 aaaatggtat tgatgttaag tctgatgttg gaaaaagttt gtttttcctt tgtgtaggaa
      121 agaacaaagg gtggcggaaa gttcagaaag ccagcgatta tggcgtaatt attctgacag
      181 aagaacaatt tatcaatttt attgatactg gtgaaattcc tcttgagcaa gactgcgtgg
      241 ttgagaatga tatcagtaaa tcgcttgaga aggatgttaa cacttacaag agtatgatag
      301 atgagttctc gctttctatc cgtactgtaa gggagtttcg ccgtagtaat gcgttgattg
      361 cggtatttgt tgatggttac gcagctggtt ggcgctttgc tgtaaaagag tgttacaagg
      421 attctttgga tataaaactt accccagtaa aaacagagtt ttttaattat gaaatttgga
      481 cgcaagggga ttcctttcag tttaatcgcg gtgatgtttt ttatagtgat cgcctggcgt
      541 atgattctta ttgcgatttt ttaaagcttg atgatgctgt tgttcttcag gtgaaatatg
      601 agtgcttttc tggttacgat tctgtaagta cttttgatgg tgtgatggat ggtgagtttt
      661 ctttttctcg tatggaaccc agacctgtct ataaattacc tgtgctgatt gaaactcaac
      721 tgtatgaccc cggaaagatt attgttgatg tttttgttcc tgatgctgcg aaaaaacaat
      781 taaaacttaa agacacctta gagctgaagc aggatgaatt tgtctgttta cttcagtcag
      841 ggtgttgttg ggttaaacga agggaagc
//
LOCUS       DAZKJR010000431          866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKJR010000431 DAZKJR010000000
VERSION     DAZKJR010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.434"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>866
                     /locus_tag="ACHOWS_005462"
     rRNA            <1..>866
                     /locus_tag="ACHOWS_005462"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          220 a          195 c          285 g          166 t
ORIGIN      
        1 gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata
       61 ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc
      121 cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt
      181 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
      241 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      301 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      361 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      421 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      481 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      541 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      601 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      661 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      721 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      781 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      841 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAZKJR010000432          848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKJR010000432 DAZKJR010000000
VERSION     DAZKJR010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.435"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHOWS_005463"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHOWS_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFQ0050856.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQTEWESFRSAGISALASPFPWGV
                     EKVELNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADHDAVTVV
                     IALPHMQPGVINVEQ"
     gene            complement(369..>848)
                     /locus_tag="ACHOWS_005464"
     CDS             complement(369..>848)
                     /locus_tag="ACHOWS_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFQ0050857.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
BASE COUNT          209 a          238 c          215 g          186 t
ORIGIN      
        1 tcctgctcaa cattaatcac gccaggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgtggt cagccagctg accggcatct aactctcgcg gtgccggagg caaatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggatg acagaagact gtcattaagc tcaacctttt ccactcccca gggaaacggg
      241 gatgccagcg cagaaatacc ggcactgcga aaagactccc attcagtctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggtaaatctt catttcccga
      361 cgcctgcctt acgcttttgc cttcggcatc tgcgaaacca gggacagact gacatccata
      421 ccttccacct ggaaatgcgg tacagcgtag agtttcacac ggaaaaatcc cgggttgtct
      481 tcgatatctt ccaccaccac ttttccgtca cgcagcggat gcgaggcctg caactcatca
      541 cccggatcgg tcatctccgt caccagaccc cggatccagt tattcagctc cagctcaagc
      601 agacggcgat ctttggtggt accgatattt tcccgctgaa tcatcttcag gtaatgcgca
      661 atgcgggaca gcaggaagat atagggaaga cgggcattaa tacggctgtt cgccgttgca
      721 tccggcgtat cgtacagtgc cggtttctgg gtggagttag cggagaagaa gcaggcataa
      781 tcccggttct tgtagtaaga gagcggaata aagcccagat tagcgaattc aaactcccgc
      841 gtttcagg
//
LOCUS       DAZKJR010000433          844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKJR010000433 DAZKJR010000000
VERSION     DAZKJR010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.436"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>844
                     /locus_tag="ACHOWS_005465"
     CDS             <1..>844
                     /locus_tag="ACHOWS_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFQ0050858.1"
                     /translation="RFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGA
                     AATTTAELWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTV
                     GDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMS
                     LFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVE
                     QVKLLLVQGDLALAEFIQSAWETTKDVPGVSALFGELAEENREFIEKTKRDELALRKS
                     IAERD"
BASE COUNT          199 a          192 c          288 g          165 t
ORIGIN      
        1 cgatttatgt cggtggcccg tgtggcggca ggtggcgggg ttgccgtact ggccgcgctt
       61 gctgccgccg cctggcaggc agagcaggct gaccgggcct tattacgttc actgaccctg
      121 accggagggg cggctgccac cacaacggca gaattgtgga aaatggccgg ggtgatcagc
      181 gatgaagccg gtggtggtat cagacaggcg gcagaaaatc tggcccgtct ggcagaaagc
      241 gggaaatata ccgccgggca gctacggatc atgggggaaa cctctcagag atggctgcag
      301 acggtggggg acgatgccgg gaaggtggaa aaagcctttg aagggattgc agcagatccg
      361 gtgaaggcgc tggcctccct gaatcagcag tataatttcc tgagcgtttc ccagttacgc
      421 catattgatg agcttgagcg cacgaaaggt aaacaggctg cggtgacgga ggcgatgtcc
      481 ctgtttgcag atgtcatgaa tgcacgtctg gagcaacttg ataaagcggc cacgccggtg
      541 gaaaaaatct gggacgatgt taaaacctgg acttctgacg catgggcatg gataggtgat
      601 catactctgg gggcactcag tctgatcact gacgtggtgg ccggaaccgt tgaacaggtg
      661 aagctgctgc ttgtgcaggg ggatctggcg ctggctgaat ttattcagtc agcctgggaa
      721 acgacaaaag atgtccccgg cgtcagtgcg ctgtttggcg agctggcaga agagaaccgg
      781 gaatttattg agaaaacaaa acgcgatgaa ctggcgctga gaaaatccat tgcggaacgg
      841 gatg
//
LOCUS       DAZKJR010000434          844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKJR010000434 DAZKJR010000000
VERSION     DAZKJR010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.437"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>844
                     /locus_tag="ACHOWS_005466"
     CDS             <1..>844
                     /locus_tag="ACHOWS_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFQ0050859.1"
                     /translation="RFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGA
                     AATTTAELWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTV
                     GDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMS
                     LFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVE
                     QVKLLLVQGDLALAEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDELALRKS
                     IAERD"
BASE COUNT          198 a          188 c          290 g          168 t
ORIGIN      
        1 cgatttatgt cggtggcccg tgtggcggca ggtggcgggg ttgccgtact ggccgcgctt
       61 gctgctgccg cctggcaggc agagcaggct gaccgggcct tattgcgttc actgaccctg
      121 accggagggg cggctgccac cacaacggca gaattgtgga aaatggccgg ggtgatcagc
      181 gatgaagccg gtggtggtat cagacaggcg gcagaaaatc tggcccgtct ggcagaaagc
      241 gggaaatata ccgccgggca gctacggatc atgggggaaa cctctcagag atggctgcag
      301 acggtggggg acgatgccgg gaaggtggaa aaagcctttg aagggattgc agcagatccg
      361 gtgaaggcgc tggcctccct gaatcagcag tataacttcc tgagcgtttc ccagttacgc
      421 catattgatg agcttgagcg cacgaaaggt aaacaggctg cggtgacgga ggcgatgtcc
      481 ctgtttgcgg atgtcatgaa tgcacgtctg gagcaacttg ataaagcggc cacgccggtg
      541 gaaaaaatct gggacgatgt taaaacctgg acttctgacg catgggcatg gataggtgat
      601 catacactgg gggcactcag tctgatcact gacgtggtgg caggaaccgt tgaacaggtg
      661 aagctgctgc ttgtgcaggg ggatctggcg ctggctgaat ttattcagtc agcctgggaa
      721 acgacaaaga atgtgcccgg cgttggtgcg ttgtttggtg aactggcaga agagaaccgc
      781 gtatttattg agaaaacaaa acgcgatgaa ctggcgctga gaaaatccat tgcggaacgg
      841 gatg
//
LOCUS       DAZKJR010000435          841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKJR010000435 DAZKJR010000000
VERSION     DAZKJR010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.438"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..286
                     /locus_tag="ACHOWS_005467"
     CDS             <1..286
                     /locus_tag="ACHOWS_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HFQ0050860.1"
                     /translation="IKVDSKGYWRNDSNVFPDRVYVGWMLYMPHILLPELIPEAAKIV
                     PVFEGEKQKGTIVVSTEDVFDGNNKEHIGKANDIEIRLLDLGLLPLLTEL"
     gene            541..>841
                     /gene="tssE"
                     /locus_tag="ACHOWS_005468"
     CDS             541..>841
                     /gene="tssE"
                     /locus_tag="ACHOWS_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFQ0050861.1"
                     /translation="MNRKSPSLYEILYGNFTGGLELNQVSEEEQVILSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTSILQGMPGTAHQLMRVLSDVLLKYEPRIKRVGVTMQEQ"
BASE COUNT          263 a          147 c          192 g          239 t
ORIGIN      
        1 gataaaagtc gactcaaaag gttattggcg taatgacagt aatgtttttc ctgacagggt
       61 atatgtggga tggatgttat atatgcccca catactctta cctgaattaa tccctgaagc
      121 cgcaaaaata gtaccggttt tcgagggcga gaagcaaaag ggaactattg ttgtttcaac
      181 cgaagacgta tttgacggaa ataataagga acacatcgga aaagctaatg atattgaaat
      241 aagacttctg gatcttggac tgctaccttt gctaacagag ctttaatcca accttatcac
      301 tcatgggtag atgacgggac agttacaccc gttaatggcc ttatttttaa cacatcagat
      361 aaaattgaat gctcaccatt aagaatacct ggagaaatta aaaaacggag attgtttcaa
      421 gagcttcttt tatttaaggg tttacaggtc aaaaatgtga atagactagt atcatgactg
      481 ttgtatagct gtattaaatt agatatttaa catgacagta atacaaattg gattattatt
      541 atgaaccgaa aatccccatc attatatgaa attctctatg gcaatttcac tggcgggctg
      601 gagttaaatc aggtcagtga ggaagagcag gttattcttt cggtgctcga taacatgcag
      661 cgtattctga atacccgggc tggaagcctt aaacatcttc cggattatgg tctgccggat
      721 atgacaagca ttctgcaggg aatgccggga acggcgcatc aactgatgcg tgtgctttca
      781 gatgttctgc tgaagtatga gccacgtatt aagcgtgtcg gggtgacgat gcaggagcag
      841 a
//
LOCUS       DAZKJR010000436          836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKJR010000436 DAZKJR010000000
VERSION     DAZKJR010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.439"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>836)
                     /locus_tag="ACHOWS_005469"
     CDS             complement(<1..>836)
                     /locus_tag="ACHOWS_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFQ0050862.1"
                     /translation="ILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESIT
                     PVTNTIPGEEDDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLC
                     HDLWQEGDGYVLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALLTDGERD
                     ALLRYRQKADHQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVNGADEAFST
                     LALHLQKAGMAQILNEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMF
                     SP"
BASE COUNT          187 a          259 c          218 g          172 t
ORIGIN      
        1 tcaggactga acatgacacc gcgtaaacgc caggcagcag tcccctgcat cagtccactg
       61 agcaaaacag ccaggctggc tttcagctcc tgccgcagtc tgagtgacaa ctgaaggaga
      121 ccgaaatcag caggttcatt cagtatctgc gccattcctg ctttttgcag gtgtaacgcc
      181 agtgtggaaa acgcttcgtc cgcaccgttc acggtacctt ccggtccgaa gatgacccct
      241 gttgcggccg ctttcgcgtc ctgccgggca ccggtagcgt tgtcagtcag ccacagccag
      301 actggtgcct gccagcccaa ctgatggtcg gctttctgac ggtaacgcag gagggcatcc
      361 cgttcgccat ccgtcaggag ggctgaaata ctcatcacct gaatgatgcc atcaaccggt
      421 tgaccggatc gcagacgctt cagttttgaa aggaagatct cgtccgggag tgactgcgcg
      481 tctccgccat aaatcagcac gtagccgtcg ccttcctgcc agaggtcatg gcaaaggccc
      541 ggggctgctt tccggaccac atccggatcc cccatcacca gcaggagtcg caccttacgc
      601 tgccagcgac gcccatagcg aaagcgcaaa tgttctgaaa cagcatcttc gctaaaaacc
      661 ggctcatcgt cgtcttcttc accgggaatg gtgttcgtca ccggagtgat actctcagcc
      721 tttttctgcc cccactggaa atacagcccg gatttaccgg cgaagtcgaa agccttctcc
      781 gtcagccagc cgagtaaaaa cagcgtggca accagtatgg cgcagaatgt gagtat
//
LOCUS       DAZKJR010000437          836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKJR010000437 DAZKJR010000000
VERSION     DAZKJR010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.440"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..>836
                     /locus_tag="ACHOWS_005470"
     CDS             136..>836
                     /locus_tag="ACHOWS_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFQ0050863.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETD"
BASE COUNT          269 a          196 c          201 g          170 t
ORIGIN      
        1 tgcggtggtg gaagaaaccg gataaaacaa cctcattgta caaatatcga tcaagtatgg
       61 cgctgctgtg tgaattctga aaaatcacag cggtcattat gcgtcagttt ttaacagagg
      121 acgtcagaaa gtgacatggc aaagctggac tggaaaaagc tggagcaggc attccgacgc
      181 gaacatgccg aaacgggaat aacattactg gactggtgcc ggaagaaaaa gattaattac
      241 aacaccgcca gaacccgtat aaaaatgggc aaaatcgatc atgaaattga tcataaaacc
      301 gatcatgaaa tcgatcatga catctcagat gaagaaccca gcaatgacgc gtgttccgac
      361 gatgaaaaat gcgcaaaaaa ctctgaaaaa aactgcgcaa attcggcaga aacgaaacgg
      421 attcgtggtt cccgactttt acctccttca aacgcttttt ctcagcgaaa cacccacgcc
      481 gtaagacacc gtggatatgc gaagtatctt gaggcagata acctcatgga tgatgcgtcc
      541 gacatggtgc tgttcgatga actggtgttc acccgggccc gcgcactttc agtaactaag
      601 gcacttaaag ggatgttcgc cgacctggaa gaggcaactg acgtggaaac ccgcgttgct
      661 ctttacgaca aaatactcaa agctgaacag gcccttgacc ggaatattgc ccgtatcgag
      721 tcaattgaac gctcattgct gacgctggac gtcctggctg agacagcacc aaaacttcgt
      781 gctgaccggg aaagaatcaa cgccgccaga gataaactca gagcggaaac cgatat
//
LOCUS       DAZKJR010000438          827 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKJR010000438 DAZKJR010000000
VERSION     DAZKJR010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.441"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(243..>827)
                     /locus_tag="ACHOWS_005471"
     CDS             complement(243..>827)
                     /locus_tag="ACHOWS_005471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFQ0050864.1"
                     /translation="ISYELTPQEYNAIELLNSNNLMWGTYNLKKLFGPAIRGKRLKNA
                     DRIPGSLPFVTAGEGNEGISAFISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAV
                     VHTEFFDRNAVIFITASIHKSSCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFME
                     TLILAVHKLVIKDVVQYTDKKLVAYRQITSEKVV"
BASE COUNT          228 a          174 c          156 g          269 t
ORIGIN      
        1 atgcgatttt taagattttg ttattaattg cggtcgcacc ttcctttctg tgtactttct
       61 gtgtactttc cgtataactt acaggattct ggatacaaaa aacccgcgca tcggcgggtt
      121 aagcagcgtg gcaatgtaac cacttttgtc atgatatgat gatttttacg attgtaaact
      181 gtttttctgc atcagggcac gataacctga ccattcccca ccctgtcagg tggggatttg
      241 cactaaacaa ccttctcaga tgtaatctgc ctgtacgcta ccagcttttt atcagtgtac
      301 tggacaacat ctttgatgac cagtttatgt actgctagaa ttagcgtctc catgaaagaa
      361 taatcagggt tattatcttt tgacggcaaa gataccatta actcgtcggc atcttttgca
      421 tagaaatttc tcccgtagtt gaactttccg gtacaagaag acttatgaat tgatgctgtt
      481 atgaaaataa cggcatttct gtcaaagaat tctgtatgaa caactgccac atggtcatct
      541 gcaccatact cataatttct gtattttgcg gagccaaaca tatctatcgt aatggtattt
      601 tcagggaata caacaacatc attactgata aatgcagaaa taccttcatt gccttcaccc
      661 gcagtaacaa aaggcaagga gccgggtata cgatctgcgt ttttcagacg cttacctctt
      721 attgcggggc caaataactt tttgaggtta taagtgcccc acataagatt attgctattt
      781 aatagctcaa tcgcgttata ttcctgtggt gtaagctcat agcttat
//
LOCUS       DAZKJR010000439          821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKJR010000439 DAZKJR010000000
VERSION     DAZKJR010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.442"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>821
                     /locus_tag="ACHOWS_005472"
     CDS             <1..>821
                     /locus_tag="ACHOWS_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0050865.1"
                     /translation="LGQKCRVVLVGDCYQQIYRFRGANNALSHPALKNADRLWLTQSF
                     RFGPAVARMANLLLQREGETREVKGCGGDDEVLLKCHAREHLQGHYTVLSRTVAGVIA
                     TALMAAMNGQKVYWVGGIEGYRTGELEDLYWFQVDMPERMHSDRLRRDYRNFEEYKYI
                     AKSTKDVEMNQSLRLLELCFPLPKKLELLRQYTVTNEQDADITVSTAHRSKGLEWPVV
                     MLDEDFQDITDPLMTEAERHDETCLLYVAVTRALRTLVLNDLMHQLAEEQQKTEG"
BASE COUNT          200 a          165 c          260 g          196 t
ORIGIN      
        1 tctggggcag aagtgtcggg tggtccttgt tggagattgt tatcaacaga tttaccgctt
       61 tcggggtgcg aataatgcgc tttcccatcc tgcactgaag aatgcggacc ggttgtggct
      121 gacacagagt ttccgttttg gtccggcggt ggctcgtatg gcaaatctgc ttctgcagcg
      181 tgaaggtgaa acacgagagg tgaaaggttg tggtggtgat gatgaggtat tgctgaaatg
      241 ccatgcgcgt gaacatctgc aggggcatta cacggtactc agtcgtactg tcgcgggtgt
      301 gattgctact gcgctgatgg cggcaatgaa cgggcaaaag gtttactggg tggggggaat
      361 cgaaggttac aggaccgggg aacttgagga tctctactgg ttccaggttg atatgccgga
      421 acggatgcat tcagacaggc tgcgccggga ctacaggaat tttgaagaat ataaatatat
      481 tgcaaaatcc acgaaggatg tggagatgaa tcagtccctt cggcttctgg agttgtgttt
      541 tccgttaccg aaaaaactgg aacttctccg gcagtatacg gtaacgaatg agcaggatgc
      601 agatatcacg gtatcaacgg cacaccggag caaggggctg gagtggccgg ttgtaatgct
      661 ggatgaggat ttccaggata tcacggatcc gctgatgacg gaggccgagc gtcatgatga
      721 aacctgtctg ttatatgtgg ctgtcacccg ggccctcagg acgctggtac tgaatgacct
      781 gatgcaccaa ctggcagaag aacagcagaa aactgaaggt t
//
LOCUS       DAZKJR010000440          820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKJR010000440 DAZKJR010000000
VERSION     DAZKJR010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.443"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          231 a          198 c          165 g          226 t
ORIGIN      
        1 gtggaatatc gggaaaacag gaagttacgt taaaagccga aggaaaaact cacaatccct
       61 tcttcaatca gcctctgaaa atcggctttt cagcatgtct tcccccattg ttcgtatacc
      121 ctgttcgatg gattattatt gatccatatg atcgcttcac gtaaacacga ttctggctcc
      181 acaccattct ggacgcgatt gttaccaaaa aagacaacca tcacaagacg tcacagacgg
      241 tgcgtgcgct tactcctgta tatctggcgc aacagaatct accagtgtaa actctctatg
      301 cttttgcgca gaagcagtgg gcctgggttc gcttatgtcc tgcatctaca agcgaatgac
      361 tgaccagaga ctcccatatc tggtcagtcg cccattttta ttttccctat acactcagac
      421 tacaatagcc gtaacaaaca gcataccccc agtcatagtg gcctctttat cttcttatgg
      481 cttgtctgtg gtactggaga tagagcaact gtgggagtac tgttatggcc cccggtaaga
      541 aaaactcttg cttaatgtac agcagaactg ttcccagaaa gaagcctgtg catattttca
      601 tagaaggaga tcacccataa cggcgttttt cacggtaata cacgccaggt aaagaatgct
      661 ggattatcaa tcaaagctcc gcacaggaag cgcttatgcc gtagagcgcc ctctggagaa
      721 aagtgctact tcttgtcgat tcagactagt tcaggaaatt atacgaaaat attacctgaa
      781 tgagctaaat gttatttacc ttcctttctg ttcattgtaa
//
LOCUS       DAZKJR010000441          819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKJR010000441 DAZKJR010000000
VERSION     DAZKJR010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.444"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..364)
                     /locus_tag="ACHOWS_005473"
     CDS             complement(<1..364)
                     /locus_tag="ACHOWS_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFQ0050866.1"
                     /translation="MARLLTIVLFFSISANAVTVNQFQRELDLADKTILWALISASTQ
                     EGRKACSLSYFSCKAAEAELGLAYMAANDNKAFLTSLSRIMMYKIDAGLSESYTCYLL
                     SKGKIIRPYLKNLNPHQLV"
BASE COUNT          225 a          162 c          138 g          294 t
ORIGIN      
        1 cgacaagttg gtgtggattt agattcttca gatatggtct tatgatcttt cccttgctta
       61 ataaatagca tgtataggat tcggaaagtc cagcatctat tttgtacatc attattctag
      121 ataaggatgt gagaaatgcc ttgttatcat tagcagccat atatgccagt ccgagttcag
      181 cctctgcggc tttacatgaa aaatagctaa gtgaacacgc ttttcgtccc tcttgggtac
      241 tggctgatat tagtgcccat aatattgtct tgtctgccaa atccaactct ctctgaaatt
      301 ggtttacagt aactgcattc gcagaaatag agaaaaaaag cacaattgtt aataaccttg
      361 ccatatttct agtccgtcac tgttaatgtg ctatctggct ctgaaaatat tagctcattt
      421 aatttttgca tgtcattaga tgaaccagtt atacaaccct cggaacagaa tcgaggtcct
      481 aaagatggac caaaagcgtt taaacaacta tgtgttaaaa atccatcacg attatacgtt
      541 tctgtatttg ctgacggtac taaacgtata ccattaggtt ttctattggt tgctccagga
      601 ctgttaagca agcctcgtcc ggtttgcttc attgagttct ccactttaga ctattggact
      661 atctttcaac atggcatcgc cttagtatac cgctggtttt ccgctcattg ttaattttct
      721 ctttcgttca tttcacttaa ttttcagttt tttccaaaag tcaatattcc cttctaataa
      781 aattttttct atggaatctt cattaatttt attattaga
//
LOCUS       DAZKJR010000442          819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKJR010000442 DAZKJR010000000
VERSION     DAZKJR010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.445"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>819
                     /locus_tag="ACHOWS_005474"
     CDS             <1..>819
                     /locus_tag="ACHOWS_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050867.1"
                     /translation="GNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGS
                     DTLKRRITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITET
                     RARTEGDAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAG
                     DTETLAAARTHSDENDKRTLVSANTYADQKETAINNRTDGLLMTERQARIAGDTATLA
                     AARTHSDENNKRTLVSANTYADQKETDINNRTDGLLMTERQARIAGDAETLATA"
BASE COUNT          266 a          218 c          201 g          134 t
ORIGIN      
        1 tcggtaatgc caccagaatt aacggtgatt catctgttgc cataggtctg acatcgtccg
       61 taaccgggaa cgactccgtg gcactgggca gtcattccag tgctaacgga aacaatatcg
      121 tatccgtcgg ctctgacaca ctgaaacgcc gcatcacaaa tctggcacag gggaaagatg
      181 accatgatgc ggtaaatctg atgcagatga ccagtgctgt taaccgggaa gctggagaaa
      241 gaataaaatc aacaaacaac ctgcagaatc agcttaatac cctctctggt caggtcataa
      301 ctgaaaccag ggcccgaaca gaaggtgatg cagcagcgct ggctgcagcc agagcccaca
      361 gcgatgaaaa cgacaaaaga acactggtca gtgcgaatac ctatgctgac cagaaagaaa
      421 ccgccatcaa taaccgtact gacggattac tgacgaccga acgccaggca cgcaccgcgg
      481 gcgatactga aactctggct gctgccagaa cgcacagcga tgaaaacgac aaaagaacac
      541 tggtcagtgc gaatacttat gctgaccaga aagaaaccgc catcaataac cgtactgacg
      601 gattactgat gaccgaacgc caggcacgca tcgcgggcga tactgcaact ctggctgctg
      661 ccagaacgca cagcgatgaa aacaacaaaa gaacactggt cagtgcgaat acctatgctg
      721 accagaaaga aaccgacatc aataaccgta ctgacggatt actgatgacc gaacgccagg
      781 cacgcatcgc aggtgatgca gaaactctgg caacagcag
//
LOCUS       DAZKJR010000443          816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKJR010000443 DAZKJR010000000
VERSION     DAZKJR010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.446"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..>816
                     /locus_tag="ACHOWS_005475"
     CDS             61..>816
                     /locus_tag="ACHOWS_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050868.1"
                     /translation="MSIDFTPCMINTYHGDIYNRTTDTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQYQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAEYRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFITEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNV
                     TGQDLSGFVLTLSRFYFEDLLNINFTDANLGDTI"
BASE COUNT          310 a          160 c          126 g          220 t
ORIGIN      
        1 cctgctctaa ttctccgatt tacaaaaatt tagcgaaata gatgaacaat aaggaatgtt
       61 atgtcaattg actttacccc atgtatgata aatacatatc acggcgatat ttataaccgc
      121 acaacagata ccgacaatgt taaaacacca gacacaccga agtggccttg cgataattgg
      181 gaagaacaac aacccattaa ttccacattc tctggagaag gatatacctc tgatcaatat
      241 gatttagcgc aataccaact tcaacaaatt aatgcgtgcc acacgaacac tacatataca
      301 aatgcagact actcaaaagt tgtggctcaa cttgtaagtc ttattactaa cattgaaacg
      361 ataagttcga cacaacttac gcaacagaca caatcaatac ttaatcagat taataacatt
      421 agatacgaaa aaaacaaaag tgctgaatat cgaatcattg ttgtcgctaa tcctaaaccc
      481 gacaaggcaa tcataactaa aattagcgta gaagagggaa taccagtaag attcagtgtc
      541 caaaccatgt tttcagatac taattttatt actgaacaac gagctgactt accgactaat
      601 ataaaagata ttcaaagcct gtatcaaaaa atgacgaagt tatatattga acacagtgaa
      661 aataaaaaca ggatgaaagt ctttgctgga accaacttta ttgatttcaa tgtgacagga
      721 caggaccttt ccgggttcgt tctaaccttg tcaaggtttt atttcgaaga tctacttaat
      781 ataaatttca ctgacgcaaa tttaggtgac actatt
//
LOCUS       DAZKJR010000444          814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKJR010000444 DAZKJR010000000
VERSION     DAZKJR010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.447"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>814
                     /locus_tag="ACHOWS_005476"
     CDS             <1..>814
                     /locus_tag="ACHOWS_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HFQ0050869.1"
                     /translation="STLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDE
                     RTHRYHYDSRHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSR
                     KPEVTWYGWDGDRLTTIQNDRTRIQTVYQPGSFTPLIRVETATGELAKTQRRSLADAL
                     QQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQIQNQMDPV
                     YTPARKIHLYHCDHRGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQL"
BASE COUNT          188 a          240 c          264 g          122 t
ORIGIN      
        1 acagcaccct cagcatgtgg ccggataacc gtatcgcccg tgacgcgcac tatctttacc
       61 ggtatgaccg tcacggcagg ctgacagaga aaaccgacct catcccggaa ggggttatcc
      121 gcacggatga tgagcggact caccggtacc attacgacag ccggcaccgg ctggtgcact
      181 acacgcggac acaatatgca gagccgctgg tcgaaagtcg ctatctttac gacccgctgg
      241 gccgcagggt ggcaaaacgg gtatggcggc gtgaacggga cctgacgggc tggatgtcgc
      301 tgtcacggaa accggaagtg acctggtacg gctgggacgg cgaccggctg accacgatac
      361 agaacgacag aacccgcatc cagacggttt atcagccggg gagcttcacg ccactcatca
      421 gggtcgaaac cgccaccgga gagctggcga aaacgcagcg ccgcagcctg gcggatgccc
      481 ttcagcagtc cggcggcgaa gacggtggca gtgtggtgtt cccgccggtg ctggtgcaga
      541 tgctcgaccg gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa agccgccgct
      601 ggctggcatc gtgcggcctg acggtggagc agatacaaaa ccagatggac ccggtgtaca
      661 cgccggcgcg aaaaatccac ctgtaccact gcgaccatcg cggcctgccg ctggcgcttg
      721 tcagcacgga aggggcaaca gaatggtgcg cagaatacga tgaatggggc aacctgctga
      781 atgaagagaa cccgcaccag ctgcagcagc tcat
//
LOCUS       DAZKJR010000445          813 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKJR010000445 DAZKJR010000000
VERSION     DAZKJR010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.448"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>813
                     /locus_tag="ACHOWS_005477"
     CDS             <1..>813
                     /locus_tag="ACHOWS_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFQ0050870.1"
                     /translation="AINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDAKQ
                     QPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNISSD
                     KESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSIDAD
                     GRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFNDW
                     DYSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCI"
BASE COUNT          236 a          171 c          180 g          226 t
ORIGIN      
        1 gctattaacg atagcagaaa caataataaa gaaggagatg ccagtcttta tacactaaaa
       61 atacaggtat catccagggg cattgagcaa gtgaattttc tcaaccagcg tccactgctt
      121 gatgcaaaac agcaaccttt cgtgaccaat acggtggatg ccgaaggtct ggccctgaca
      181 cacaatggta aatcattact ctggagttcg gagttaggtg ctccactgcg tctgagcact
      241 ctcgatggcg taatggagaa agattttacg tctttatttc ctgctcgctt taatattagt
      301 tctgacaaag agagcagcaa tggtattcgc agcggcaacg cctgggaggg cctgacagtt
      361 acaccagatg gaaaatcact ttttatcgcc gttgaaagca gccttaaaca ggatgggcct
      421 attgcaagtc caataaattc aggcacatca cgcttactgc aattttctat agatgcagac
      481 ggtcgacctt caaagcaatt gcatgaatat ctctatatta ctgatcctgt tccgcaagtg
      541 agtaagttcg gtattaatga taatggcgta tccgaggtca tggcgctgaa tgatcaccaa
      601 ttgttggtca tcgaacgttc cggcagaaac gtcagtgctg ggttcaatga ctgggattac
      661 tctgtcagag tttatatggt cgatttaact gctgccagcg atatcaagaa tattgacagt
      721 ctgcaagatt ggtccaataa atccacgtta caacccgtca gtaaaaaatt attaattgat
      781 tttgctgatt acacttcctc tgcggattgt att
//
LOCUS       DAZKJR010000446          806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKJR010000446 DAZKJR010000000
VERSION     DAZKJR010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.449"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..762)
                     /locus_tag="ACHOWS_005478"
     CDS             complement(<1..762)
                     /locus_tag="ACHOWS_005478"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050871.1"
                     /translation="MGSDIPVFYSWTGPFMDSVVSFISAYFPIFLVGAVFGTLMNASG
                     AALTIAHFISDKMGEKRAIIAVIIASFLLTYGGISVFVVVFAVLPIARTIFIKSNYPQ
                     RLLPSAICAGCLPAYVAPGSAQFINAIPIPVFNTTIYSGLVIGIVGTAVWLLFAVWYM
                     SAKIKNAKIKGEGYGTEVSNDCNLETGKVTLAFIPILIVIIGNAFLTYFFQIDEVVDY
                     YRDFGGVQGTWAILISIAIAIVVTICIYWNTFKSDI"
BASE COUNT          302 a          163 c          116 g          225 t
ORIGIN      
        1 tatatcactt ttaaaagtat tccagtaaat acaaatagtg acgacaatag caattgctat
       61 ggaaattaaa attgcccatg ttccctgaac acctccaaaa tctctgtaat aatcaaccac
      121 ttcgtcaatc tggaaaaaat atgttaaaaa tgcatttcct ataataacta tcagtatagg
      181 tataaatgcc aaagtaactt ttccagtctc aagattacag tcattactta cttctgtacc
      241 atatccttct ccttttatct tagcattttt aatttttgca gacatatacc atacagcgaa
      301 caataaccat acagcagtgc caacgatacc aattactaaa cctgaatata tggttgtatt
      361 gaacacagga attggtattg catttataaa ctgggcagaa ccaggagcta catatgctgg
      421 taagcaacca gcacagatag cactgggtaa taatctttga gggtaattac tttttataaa
      481 tatagttctc gcaataggta aaactgcaaa aaccacaaca aaaactgata ttcctccata
      541 agttaataaa aatgatgcaa taataactgc aataatagcc ctcttctcac ccattttatc
      601 tgatataaaa tgggcaatag ttaatgctgc tccacttgca ttcattaatg ttccaaaaac
      661 agcaccaaca agaaatatag ggaagtatgc ggatataaaa ctcacaaccg agtccataaa
      721 tggaccggtc catgaataaa agacaggaat atcacttccc ataaagacag ccagcattgc
      781 catcaaaggg gatactatca gaactg
//
LOCUS       DAZKJR010000447          804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKJR010000447 DAZKJR010000000
VERSION     DAZKJR010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.450"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..768
                     /gene="ompT"
                     /locus_tag="ACHOWS_005479"
     CDS             <1..768
                     /gene="ompT"
                     /locus_tag="ACHOWS_005479"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFQ0050872.1"
                     /translation="DWKFNSAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDW
                     MDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTAR
                     GGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSG
                     WVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKK
                     GNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          258 a          168 c          180 g          198 t
ORIGIN      
        1 gactggaaat tcaatagcgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
       61 cagatatcta tcggggctgc tggctggaca actctcggca gccgaggtgg caatatggtc
      121 gatcaggact ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct
      181 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac
      241 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
      301 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
      361 ttcccgaatg gagaaagggc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc
      421 ttgactggaa gttatcgtta tgaagatttt gaactcggtg gcacatttaa atacagcggc
      481 tgggtggaat catctgataa cgatgaacac tatgacccgg gaaaaagaat cacttatcgc
      541 agtaaggtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtcaca
      601 cctaacgcaa aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat
      661 acttcacttt atgatcacaa taataacact tcagactaca gcaaaaatgg agcaggtata
      721 gaaaactata acttcatcac tactgctggt cttaagtaca cattttaaga acgccaacta
      781 aaatttcccc gaggtgaaaa tcgc
//
LOCUS       DAZKJR010000448          793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKJR010000448 DAZKJR010000000
VERSION     DAZKJR010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.451"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..701)
                     /gene="tssG"
                     /locus_tag="ACHOWS_005480"
     CDS             complement(<1..701)
                     /gene="tssG"
                     /locus_tag="ACHOWS_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFQ0050873.1"
                     /translation="MERESQPARTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIDNRSGLGAGKR"
     gene            complement(665..>793)
                     /locus_tag="ACHOWS_005481"
     CDS             complement(665..>793)
                     /locus_tag="ACHOWS_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFQ0050874.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHNLHVPG"
BASE COUNT          163 a          224 c          234 g          172 t
ORIGIN      
        1 atgcgttttc cggcacccag cccactgcga ttatcgatat ggaccttaac cggatcgggt
       61 tcagtgataa gtgcgcgggt atccggtgcc agcagactca ccagagcccg gatgccttcc
      121 gcattgcggg tgggtaatcg catggcaccc agtaaggcca ggaaacggga aaccggcgtt
      181 gccacctgtt cagcggtgcc gggtataccg agtccgacca gccccagcag acactgcgat
      241 gtggcatcgc gtcccccctc ctcaaaagtg gccggatacg catattttcg ccagatccgg
      301 tagtactgcg tggtgatgcg gtggctgaaa atatccagaa atgaggtgag cgcctcatgt
      361 ccttcacgac gctgcgtaat atcatccagc caggacgtcg gcagaggtga gtccacccca
      421 tacattccga gaaatgtcgt gcggattgtt gggggtaatg tcggacggtc ttcgtctatc
      481 tccaccgctt tcagggtgct gacagggaat cccatcccgg gccagggcct gaatcgcacc
      541 ggatcatcgc ccggatggct ggtcgtgccc agttttggcc cgtccggatt ttcctgctcc
      601 agcagctggc agaaacgata aaaactggcc cgccagatat cgttgttaag ggccagggcc
      661 tgcgtcagcc cggtacgtgc aggttgtgat tctctttcca tctcagtcgt ttccctgtgg
      721 gttgcagtac cagtgtgagc tggttgaaaa ggtggacatc agcgtagagc gcaaagaaac
      781 ggtgcagcat ctc
//
LOCUS       DAZKJR010000449          775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKJR010000449 DAZKJR010000000
VERSION     DAZKJR010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.452"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..402
                     /locus_tag="ACHOWS_005482"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACHOWS_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            394..729
                     /locus_tag="ACHOWS_005483"
     CDS             394..729
                     /locus_tag="ACHOWS_005483"
                     /inference="COORDINATES: protein motif:HMM:NF026979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain fold toxin domain-containing protein"
                     /protein_id="HFQ0050875.1"
                     /translation="MAKTRDLATTISDKFKQLFQCDKFAAALKSKMQQAGVNGEHLIL
                     ETKNKQGAFGNIWSDRIGKNIATNGHHEAIKVGDTVFDNMNPSGIKYEDWLNDIYSPG
                     EYTITPSRF"
BASE COUNT          236 a          157 c          199 g          183 t
ORIGIN      
        1 ccgtcggtgg ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc
       61 cgtccgctga tgctctttaa cagtgaaggt aaaaccgtgt ggcgaccgtg acaaaccagc
      121 ctgtgggggc tggcactcag tctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gacccgatag ggctgagagg cgggataaac aattatgcat atgttcataa tccccttggt
      361 tatattgatc cattaggatt aaccatttgc gatatcgcaa aaacaaggga tcttgcaaca
      421 acaataagtg ataagtttaa acagcttttt caatgcgata aatttgccgc agcgttaaaa
      481 agtaaaatgc aacaagctgg ggtaaatggt gagcatctca tactcgaaac taaaaataaa
      541 caaggtgcct ttggcaatat atggagtgat agaataggta aaaatatagc aactaatggt
      601 catcatgaag caataaaagt aggtgataca gtttttgata atatgaatcc ttctggtata
      661 aagtatgaag attggctgaa tgatatatac tctcctggcg aatacaccat tactccatca
      721 aggttttaat tatgaatgat attgttaaac tgattgatat gatagaaaaa agacc
//
LOCUS       DAZKJR010000450          767 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKJR010000450 DAZKJR010000000
VERSION     DAZKJR010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.453"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            188..>767
                     /locus_tag="ACHOWS_005484"
     CDS             188..>767
                     /locus_tag="ACHOWS_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050876.1"
                     /translation="MSPDIISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPV"
BASE COUNT          244 a          153 c          144 g          226 t
ORIGIN      
        1 attgtttcat tagtaataat cacttaaatg tatcgatgaa acaggtatga cttagcggga
       61 gtgattgttt ctgagtgaac agagaccact ttcgcctgat aaatgaaaaa tgagcgattt
      121 ttgcaagtta gtttttctat agccgagtac gctcagcgcc tcgcttgatt acaatgaggt
      181 tgctgctatg agtcctgata ttatcagttc tgctgtcaat cgcttctcag gttggccatc
      241 taatgatcct ctaaaaacag ataaagaatc aatgacatca cccatgactg tgcaggtaat
      301 aaagatgatt gccgccatcc ttgacccaca tcggatagtc gttggaatcg atacacttta
      361 tacaacgcag cgcgaaacat ttacctcaga gagtcaacag ctcattgaat cagcattgca
      421 aacacttcat gtgatgaatt taaataacct ggaaacaaat ctcgatgagg aaggtcacta
      481 tctggctttt ggatataacg ttgacactcc tgacagaata accgtttctg cttataatga
      541 aaaaaagcct ggctgctatg gccaatgtct tttaatacag gatgacgaaa cagacttatt
      601 taacctgata aaagttaaaa tttttgaagc caggcataat tatgtcgctc aattgatgag
      661 cgatccggaa tttatgttca aatttagtta tagcgctcaa caaaatagat tagaacctca
      721 tcttctggtt cccacttatt tccctctaaa aacaaacgaa ccggtta
//
LOCUS       DAZKJR010000451          767 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKJR010000451 DAZKJR010000000
VERSION     DAZKJR010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.454"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>767
                     /locus_tag="ACHOWS_005485"
     CDS             <1..>767
                     /locus_tag="ACHOWS_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFQ0050877.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPLIDGTEVAIVFDGGDPDRPYIAYALHDSEHPDHVTSDNHTRNVWRTPANNKLRME
                     DKRQEEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTDEHGAVRAAKGLFLTADA
                     QARAQGPVLEMAAAISQINQANSQMQALNSAAEAAGAL"
BASE COUNT          179 a          207 c          236 g          145 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgcttat cgacggaacg gaagtggcca ttgtatttga cggcggagat
      361 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
      421 agtgacaatc atacgcggaa cgtgtggcgc acgccggcga acaataagct gcggatggag
      481 gataagcgcc aggaagagca tatcaagctc gcaacggaat atggcaaaac acagctgaac
      541 atgggacacc tggtcaacag ccggcgtgaa aagcgaggtg ccggttttga actgcgaacg
      601 gatgagcatg gggctgtacg tgcagcaaaa gggctgttcc tgacggcaga tgcccaggcc
      661 agagcccagg ggccggtgct ggaaatggcg gccgcgataa gtcagattaa ccaggctaac
      721 agccagatgc aggccctgaa cagcgctgcc gaagccgccg gcgccct
//
LOCUS       DAZKJR010000452          762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKJR010000452 DAZKJR010000000
VERSION     DAZKJR010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.455"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..>762
                     /locus_tag="ACHOWS_005486"
     CDS             113..>762
                     /locus_tag="ACHOWS_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050878.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     IKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSKDL
                     AQSAAAVKDLVNYFNELYIIKVNSGKIGKTVDENWVRLIKNMTVLRNDIYTKGDKI"
BASE COUNT          266 a          125 c          154 g          217 t
ORIGIN      
        1 taaatagtcg cagtatttta atttaatttc atacttgctt atgaatatta ccaggaaata
       61 gccacattac tatttaatta tctgagataa actcaacgtc aagaggtggg ttatgattag
      121 atatagtgga atatttctat cgctgttaat gctttcaggt tgtgcaactg aagcattgaa
      181 aaacgaacaa gctgaaactg ttattactgc gggtaacaaa gcagtagcgg cgaatgcaag
      241 tttcattaaa caatactatg cttctcgtga aaacttcctt gttaatttct acgcgactaa
      301 tccggattgt gccctgcgac ctgattatac cattgatata ggcggtgatg ggaatggcgg
      361 aaaattatgc cagcgttcag ggccaaatac aaaacaagta caattagtcc ctttcgagaa
      421 agaaaaatta ctggcctcaa tggaagtcat taatagcatg agcgcttatg tggaacttct
      481 gacaaaggat atggcaacta cggaaagtca gcaagtggca ttacttaaac aggctctgga
      541 tcacgcaaac aacgcacaaa cactacttgg tgtttcacat agcaaagatt tagctcaatc
      601 tgcagcagcg gttaaagatt tggtaaatta tttcaatgag ttatatataa ttaaagtcaa
      661 ttctggaaaa ataggcaaaa cggtagatga aaactgggta agacttataa aaaacatgac
      721 tgttttgcgt aatgatattt atacaaaagg cgataagatt ga
//
LOCUS       DAZKJR010000453          757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKJR010000453 DAZKJR010000000
VERSION     DAZKJR010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.456"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..450
                     /locus_tag="ACHOWS_005487"
     CDS             <1..450
                     /locus_tag="ACHOWS_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFQ0050879.1"
                     /translation="RGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAV
                     FRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRSQAR
                     EQFTGAVDRIFDTAESVCVASVVRTALRAVRNTVVSVARAVWNWIFF"
BASE COUNT          165 a          211 c          195 g          186 t
ORIGIN      
        1 cggggacatc agcaggtgct gtttgtggtg acgcaggccg acaaaacgga gccctgccat
       61 gaatgggata tggccggtat tcagccttct cctgcacagg cacagaatat tcgcgaaaaa
      121 acggaggcgg tattccgtct gttccggccc gtacatccgg ttgtggccgt atcggcccgc
      181 accggctggg aactggatac gctggtcagt gcgctcatga cagcgcttcc cgaccatgcc
      241 gccagtcccc tgatgacccg actgcaggac gagctgcgca cggaatctgt ccggtctcag
      301 gcccgtgaac agtttaccgg tgcggtggac cggatatttg acacagcgga gagcgtctgt
      361 gttgcctctg ttgtccgtac ggccctgcgc gctgttcgta acacggtggt ctctgttgcc
      421 cgtgcggtat ggaactggat tttcttctga acctgtcgtg actgatgccc tccctgactc
      481 tgagtctgct cacaaaagcg ctgttttcgt tactgtctct cttgtccgtg caatagccaa
      541 acaatagaat aaaacgatcg atacctattt tatcgatcgt ttatatcgat cgataagcta
      601 ataataacct ttgtcagtaa catgcacaga tacgtacaga aagacattca gggaacaaca
      661 gagccacacg tcagaaactt ccgtcagccg gacctctggc actgtaaccc tttacctgcc
      721 ggtatccaca tctgtggata ccggcttttt tattcac
//
LOCUS       DAZKJR010000454          756 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKJR010000454 DAZKJR010000000
VERSION     DAZKJR010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.457"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>756
                     /locus_tag="ACHOWS_005488"
     CDS             <1..>756
                     /locus_tag="ACHOWS_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFQ0050880.1"
                     /translation="ANVGIPADKVTQDGEYTVKAEITDPAGNTSGQGKATQFGVDTVA
                     PSEPALKAENDGSVSATLPDGANKGDKVEVTFTDEEGNEQKVTLEKGDGNWSSDKPEL
                     IPDSTDNKVTVPADKVKNNTEVTATAKDPGGNESAPVTVTSKTDGVADAPALTIPEVA
                     DSVANAAELKDGLQAEVKLPAGTVEGAVITLTVTHPDKTTRTETHTVSKDEAADGTVS
                     MVVPKGSVVDGQNSVSVSLTQGSNPAKAGNKVEF"
BASE COUNT          222 a          154 c          237 g          143 t
ORIGIN      
        1 gcgaatgtgg gcattcctgc ggataaagtg acgcaggatg gggaatacac agttaaggct
       61 gaaatcacag acccggcagg caacaccagt ggtcagggta aagctaccca gtttggcgtg
      121 gatactgtag ctccgtcaga accagcactg aaggctgaga atgatggttc tgtatcagcg
      181 actctgccag atggtgcgaa caaaggtgac aaggtggaag tgacctttac ggatgaagag
      241 ggtaatgagc agaaagtcac gctggagaaa ggggatggca actggagttc cgacaagcct
      301 gaactgatcc cggacagcac cgataataaa gtgacagttc cggcagacaa agtgaagaac
      361 aacacagagg tgacggcaac agcgaaagat ccgggtggta atgaaagtgc tccggtcact
      421 gtgacgtcaa aaactgatgg tgtggcagat gctccggccc tgaccattcc agaagtggct
      481 gatagtgtgg ccaatgcggc tgagctgaaa gatggtttac aggcagaagt caagttgccg
      541 gcaggaacag tggaaggtgc tgtcattaca ctgacagtga ctcatccgga taaaaccacc
      601 agaaccgaga cgcatactgt tagcaaagat gaggctgcag acggtactgt gtcaatggtt
      661 gttccgaagg gaagtgttgt tgacggtcag aacagtgtga gtgtcagcct gactcagggc
      721 agtaacccgg ctaaagcggg gaataaagtg gagttt
//
LOCUS       DAZKJR010000455          754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKJR010000455 DAZKJR010000000
VERSION     DAZKJR010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.458"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..438
                     /gene="tssJ"
                     /locus_tag="ACHOWS_005489"
     CDS             <1..438
                     /gene="tssJ"
                     /locus_tag="ACHOWS_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004163322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFQ0050881.1"
                     /translation="GTISITKSIFYKKIKTLHLDFTPRTAINTDGAQTPLATMVRVYQ
                     LRDRKAVDAADYQMLLRRADTVLKDDVLASKELLVMPGGSVTLNMPMDEDAQFVAVVG
                     LFNRPDQKDNRWRLILTRDDLNPDKPRTIELGDGWLSLVPVKE"
     gene            443..>754
                     /locus_tag="ACHOWS_005490"
     CDS             443..>754
                     /locus_tag="ACHOWS_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFQ0050882.1"
                     /translation="MDNRSAFFDGQPRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LE"
BASE COUNT          176 a          191 c          217 g          170 t
ORIGIN      
        1 ggtacgatca gcatcacgaa atccatcttc tacaaaaaaa tcaaaacgct gcatctggat
       61 tttaccccgc gtacggccat taacactgat ggggcacaga cgccactggc aaccatggtg
      121 cgggtttatc agctcaggga tcgtaaagcg gttgatgccg ccgattacca gatgctgctg
      181 cgccgggccg atacggtact taaggacgac gtgctggcct caaaagaact gctggtgatg
      241 cccggcggca gcgtgacgct caatatgccc atggatgaag atgcgcagtt tgtggctgtg
      301 gtgggactgt ttaaccggcc ggatcagaag gacaatcgct ggcgcctgat actgacccgt
      361 gatgacctca atccggataa gccccgcacc attgaactgg gtgatggctg gctcagtctt
      421 gtgccggtaa aggagtgacg tgatggataa ccggtcagcg ttttttgacg gacagccccg
      481 ttactttctg gaaattaatg gcagtgcagt aaagcctgat gttttacgtt ttcgtggcag
      541 ggaagcactg agtgagccgt tccgatggga tatcgggttc acctcccgga atgcgaacat
      601 tcctccggaa gaggtgctga tgaaatatgc gtcgttgcgg atgcgtaacg gcaaaaatgt
      661 ccatggcatt gtgacccgtc tggaatggct gtccacatcg aaagaccagt cccactaccg
      721 gcttacactc agctcccgtc tggcactgct ggag
//
LOCUS       DAZKJR010000456          753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKJR010000456 DAZKJR010000000
VERSION     DAZKJR010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.459"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..461
                     /locus_tag="ACHOWS_005491"
     CDS             <1..461
                     /locus_tag="ACHOWS_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFQ0050883.1"
                     /translation="GISGFNNLGAYNYFIRGMNAPRQVYYDVLIESDTEMVVPDDPIR
                     YRDVHWRLVWEDTRYSDGIIPDESEYFLDDAPGKRITCPSGELCPHSGHWATIAGGHQ
                     QFIDVQAGQLMPEATKYQSNMYADEIHLPAIWNLLRRDDEGSVYVKTGES"
     gene            539..>753
                     /locus_tag="ACHOWS_005492"
     CDS             539..>753
                     /locus_tag="ACHOWS_005492"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFQ0050884.1"
                     /translation="MDGQRYFGESLSVQQPAAVPLFDTWPDSRIAFAGPWVMEMNSIT
                     DFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT          189 a          162 c          195 g          207 t
ORIGIN      
        1 atggtatcag cggatttaat aacctcggtg cttataatta ttttattcgt ggaatgaatg
       61 ctcctcgcca ggtgtattac gacgtattaa ttgaaagtga cacagaaatg gtcgtacccg
      121 atgacccaat ccgttaccgg gacgttcact ggcgtctggt ctgggaagac acgcgctaca
      181 gtgacggaat tattcccgat gagtcagaat actttctgga tgatgcacca ggcaaacgca
      241 ttacctgccc gagtggggag ctttgcccgc attcaggtca ttgggcgacc atagccggag
      301 gacatcagca gtttattgat gttcaggccg ggcagttgat gccagaagca actaaatatc
      361 agagcaatat gtatgcagat gaaatccatc ttcctgcgat atggaacctt ttacgccggg
      421 atgatgaggg gagtgtttat gtaaaaacag gggaaagtta aataactgat caacgctttt
      481 tgacggacag atctgttacc acctgatttg ctcaacaaaa gtgacattat ttgctctggt
      541 ggatggtcag cggtatttcg gtgagagcct gtctgttcaa caacctgccg ccgtgcccct
      601 gttcgatacc tggcctgatt cgcgtattgc atttgcgggg ccatgggtta tggagatgaa
      661 cagtataacg gatttcagag aaagactgtg tgagcttgaa gctgcgcttc cgtcagtttc
      721 ctggatgata tcttcttcct cgcttactga act
//
LOCUS       DAZKJR010000457          745 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKJR010000457 DAZKJR010000000
VERSION     DAZKJR010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.460"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..>745
                     /locus_tag="ACHOWS_005493"
     CDS             39..>745
                     /locus_tag="ACHOWS_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFQ0050885.1"
                     /translation="MNSYLKISLAGITMLSCLSPTLVCAEQPHGFIDYRHEYLDDTRT
                     HADRVEFGTFFSNGIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKK
                     FWLEPMFWLDTTQYWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRN
                     NRRYDVWIDYRPQKTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKMGSWIPYMS
                     IADYKGVDKTSSNRQIR"
BASE COUNT          237 a          140 c          154 g          214 t
ORIGIN      
        1 gatcaccaaa aatatatttt attctgaaag ggaatattat gaattcttat cttaaaatat
       61 cacttgcagg aataacaatg ttatcctgcc tgtcacctac actagtctgt gcagaacagc
      121 cgcatggatt tattgattat cgccatgagt atcttgatga tactcgcacc cacgcggacc
      181 gcgttgaatt tggtacattt tttagtaatg gtatcggttt gatgggagaa ttacgctata
      241 acaccgatga aggtgataaa gataagtggg acccctcaca atttggcaat aacggaacag
      301 gattatctgt tgtttaccgc tttaagccgc tggatgacaa aaaattctgg ttagaaccca
      361 tgttctggct tgataccacg caatattggt caacttacga atatggtctt tctgcgggtt
      421 atgacttcag caaagaatgg aaagtttcag gtcggtttcg ttatgacatg gataaagcca
      481 ccgataaatc caaaggttat ggcaacgatg atcgtaataa ccgccgttac gatgtgtgga
      541 tcgactatcg accacaaaaa accaacttcc aatatcagtt aaacctggtc tattacaata
      601 atggttattt aacctggaat gatggccata aaaactatac cgcatcattt aaagtagggt
      661 acaaaatggg atcgtggata ccctatatga gtattgcgga ttataaaggt gtggataaaa
      721 catcaagtaa tcgccaaatc cgctg
//
LOCUS       DAZKJR010000458          735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKJR010000458 DAZKJR010000000
VERSION     DAZKJR010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.461"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..260)
                     /locus_tag="ACHOWS_005494"
     CDS             complement(<1..260)
                     /locus_tag="ACHOWS_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050886.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTI"
     gene            complement(342..719)
                     /locus_tag="ACHOWS_005495"
     CDS             complement(342..719)
                     /locus_tag="ACHOWS_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050887.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
BASE COUNT          187 a          224 c          177 g          147 t
ORIGIN      
        1 tcaatagtga cattcaccga ccacagcccg tcggcagacc ggcagtaaaa cgccagcgtg
       61 tccgttaccg gaaacaactc tgacgcccgg gtcagggagc cgaaatacgc attctgcggc
      121 cgtaacgcct ccggcaactg ccagaaggtt tcagtgacag ccagaacaaa agcatgccac
      181 tgcttttccg gtattgtgga aacctgctca agggtatata ccaccggctc acgggccgga
      241 cgtgaagata actcagacat ggaacattcc tcatggttaa aacgaaacaa cggggaatgt
      301 ctcccggacg gagaactccc ccgtcagggt caggtcatga attacatcat gtcgtgccgg
      361 gcaaaatatt catccgctcc ggccaccatc tttccggtac tgatactgtc agcccagatt
      421 tcatgattaa tttcacgccc cagagccatc agactgtcga gtgcctgacg ggcctgaatt
      481 cccggtgcgg aaatctcctc ctgctccagg tcatacaaca gccagcccag cgcctcgccg
      541 gtatcccaca gatacacgta taccagtgaa ggctccgtct catcatccgg ctccggggta
      601 atacccagcc gttgccaggc acgtaaccgc atcagctcgc cttccatctg actgatatga
      661 gccagcagag cagactgcgt ctgaagcgct gaataatgag caattaaagg tgaatacatg
      721 atgacctccg tctct
//
LOCUS       DAZKJR010000459          726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKJR010000459 DAZKJR010000000
VERSION     DAZKJR010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.462"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          186 a          153 c          153 g          234 t
ORIGIN      
        1 atatgacggt taacaacatg ataaataatg gtttttatgc taagagtgag acttttggag
       61 gaaatgggcg cagaaccccc tttacggcag ctgccagacg tgcggcgcgt tggggtgctt
      121 cccagcggtc gtcactgtaa ttagcaactt cagccagata atccttttca aggttttcag
      181 ccgtgtaaca cctgccgcct aaaaaaaacc gcatggcctc ccgggttttg tctctcacat
      241 atcatccctc attaatgcta atgtatgcat acattagcat taatatctga cagcaacaag
      301 cggcgaagac tacccctaat caagtacaac cgcacatgta cttcttagta tcgaagatga
      361 gtttctcata gagaaatgat tttacagccc tccttttctc tggagggcgt tatcatttga
      421 aatttacgtc acgtaactcc gtacatccgt ttataatgtt gcttccggct ctactgctgg
      481 ttgggtgttt agttttggtg ataattctca tgtttcgttt ctcagcgtgg ggagtacaga
      541 ctttatggtg aatgattggt tgcttttctc tgttagtgta ttagtttttt tagtgataat
      601 cctgatgtta catttgtcct taaaaacagc cagtcttctt tctgaatgct ctgaatatcg
      661 cacgaaatgt gataatcttt atcatctatg ccagatgcaa gaggaagagt tagaacatct
      721 acgtgc
//
LOCUS       DAZKJR010000460          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKJR010000460 DAZKJR010000000
VERSION     DAZKJR010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.463"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(52..285)
                     /locus_tag="ACHOWS_005496"
     CDS             complement(52..285)
                     /locus_tag="ACHOWS_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050888.1"
                     /translation="MARSDYDFINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(380..>725)
                     /locus_tag="ACHOWS_005497"
     CDS             complement(380..>725)
                     /locus_tag="ACHOWS_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFQ0050889.1"
                     /translation="FGCVYSLKASLSGVPDTYRYHLSHRIRRMVGSENTSLPYQQVAR
                     EVKAPRERLKYALEAGLLVTALDGLFWSGSQRIAADVLRLRKAGMPVVTTSVEVHDNL
                     TGTTRKIPAYHL"
BASE COUNT          175 a          225 c          158 g          167 t
ORIGIN      
        1 aagaaccccc tgccggcaat cacactgcct gatggcagca taatccactt attaattctc
       61 atccccggag acaagccctg aaaaccactc gggatgttca ctgtatgctg cgttcagagc
      121 atcccaggaa acgttcatac agaagctgtc ctgcaatttg caggcaacca cctctgccag
      181 gcggttccgg ttatccgcca gtccggcata acctctctct gccagccact cattcagttc
      241 atgttccaga gacagattga taaaatcgta atcggacctt gccattcccg tttccttaac
      301 acatgtcttc agactcaggg ataaagtata aaaatgtcgc cctgctgctg ccagagcaat
      361 attccccctg atggccgtat cagagatggt atgccggtat tttacgggtg gttcccgtca
      421 ggttatcgtg tacctccacg ctcgtagtca ccaccggcat tccggccttt ctcagcctca
      481 aaacatcagc tgcaatacgc tgactaccag accagaacag gccgtccagt gcagtcacca
      541 gtaaccccgc ctccagcgca tacttcagcc gttcacgggg cgctttcact tcccgggcaa
      601 cctgctgata tggcaatgat gtgttttcac tcccgaccat ccggcgaata cgatgagaca
      661 gatgataccg gtatgtatcc ggcacacctg aaaggctggc cttcaggctg tacacgcagc
      721 caaag
//
LOCUS       DAZKJR010000461          722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKJR010000461 DAZKJR010000000
VERSION     DAZKJR010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.464"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..>722
                     /locus_tag="ACHOWS_005498"
     CDS             31..>722
                     /locus_tag="ACHOWS_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0050890.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKV"
BASE COUNT          240 a          124 c          161 g          197 t
ORIGIN      
        1 tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa tattgttgct
       61 aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg tgtagaaatc
      121 tatgctgctg ccaaacaagg tgaacccgat ccagaattaa acacatcttt aaaattcgtt
      181 attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc aattgataaa
      241 gccaaaggcg gcggagatga aacgttcgta cagggacgtt atgaaggctt tggtcctaat
      301 ggctcaatga ttattgctga gacattgact tcaaatgtta accgtacgat tgctaacgtt
      361 cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt cagctatatg
      421 tttgacaata cgggtgtgat tgtatttaaa ggaacagacc ctgaccatat ttttgaaatt
      481 ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa cattgttatt
      541 tatactgaac ctaccgacct tcataaagga atcgcggctc taaaagcagc tggaatcact
      601 gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct ttccccagaa
      661 gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga tgtacaaaaa
      721 gt
//
LOCUS       DAZKJR010000462          719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKJR010000462 DAZKJR010000000
VERSION     DAZKJR010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.465"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(407..>719)
                     /locus_tag="ACHOWS_005499"
     CDS             complement(407..>719)
                     /locus_tag="ACHOWS_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050891.1"
                     /translation="DKERVYDGETLAELITENKILSSRESKENLIGLFTGRHKYTLLQ
                     CIEKLGVLVDYVISPADDIKNVMMLYGNNAENKNRRRLIYNALTIICGDDTKNNQSDP
                     R"
BASE COUNT          240 a          147 c          106 g          226 t
ORIGIN      
        1 tttgtattgg atgttcaatg tgtacttctg cacagacaaa aaaataaaac ccacaaaata
       61 aaatatcaat cccaataaaa aattttaaaa tacagcgtga aacaccacgt tatggatatg
      121 ttttcatatc accagaagca tatttcacac atacaataca ggaacaattc atcatataat
      181 cagggttact gttcactcct cacggttatc cgcgagacga taaaacatga cactaatcac
      241 aaaaacgaga gatagtattt gcaaaatgca aacaagataa aaatcaatgt gttgcgattt
      301 tttatttttt cagacatgca tgccccctct gctattgttg ctagccgact agcaacaata
      361 gcagggatat acaaacatcg gatgccaaaa acacgttccg ataatatcat ctagggtctg
      421 actggttgtt tttagtatca tcaccacaaa tgatggttaa tgcgttgtag atcagtctgc
      481 gtctgttctt gttttcagca ttatttccat acaacatcat gacgttttta atatcatcag
      541 ccggtgatat tacataatca accaatacgc caagcttctc aatacattgc agtaaagtat
      601 atttgtgtct gcccgtaaat aatcctatca ggttttcttt actttcacga ctggataata
      661 ttttgttctc tgtaattaat tcagccaacg tttcaccatc gtaaacacgt tctttatct
//
LOCUS       DAZKJR010000463          709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKJR010000463 DAZKJR010000000
VERSION     DAZKJR010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.466"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          240 a          129 c          138 g          202 t
ORIGIN      
        1 aattgggaca gttttttata actcattaat ccctacctgt tgctttattt tcattatgaa
       61 aagttaccaa atcttagcac ctcagtcagg agagtcaggt catcgataaa gaatattcca
      121 gtagagtggc gctcatgatc aaagataaca gcaaagttga tgacagcgct gaccgactgt
      181 catttacaga aaccctgttt gtagcacatt ccatcttacc tgacccagac aaagacaaag
      241 acaaaagaga atgattgtta tttacttttc aaaaaaaagt gtcatattgt ccatataaag
      301 tcaagaggta ttttacgtta tgtcactgat gcaattgtta gtcaggatct catggttgtc
      361 gctcatgtat aacctgagac ttgaacgcac actaatagca ctgacggtca tacatcggga
      421 aatgtgagga ggcataagaa tgaattgtga taataatcac agaaatgaag aattcattgt
      481 tacctttgat aaaggcaaca agcaagacaa ttcaagacga aaacacgata attttcctat
      541 agaggtagaa tcctccgtag agcaggagac acactgtatc acaaataata agtcggcttc
      601 cggtatagta acacatgact atgatgccga ttatatttgt ggttgtggtg aaattatgtg
      661 tcctggttgc ggtcatgacc tataacaaac atttttgcaa actgatacc
//
LOCUS       DAZKJR010000464          706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKJR010000464 DAZKJR010000000
VERSION     DAZKJR010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.467"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>706
                     /locus_tag="ACHOWS_005500"
     CDS             <1..>706
                     /locus_tag="ACHOWS_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0050892.1"
                     /translation="KVAHLRAIMGFGIKMKLLPQTENPLVEASVASTEKKKKTLSDNQ
                     MLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLDVLRYTGMRFNQLKHIRVRD
                     VCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLERARQCGAGDDDYLFDVCRFS
                     EPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRFRHTLATRLMKYPERNLHLV
                     KSILGHRSLTTTMGYV"
BASE COUNT          183 a          151 c          195 g          177 t
ORIGIN      
        1 acaaggtggc ccacttgcgg gcaattatgg gattcgggat caagatgaaa ctgctgcctc
       61 agacagaaaa cccgttggtg gaagcatcag ttgcgagtac tgaaaaaaag aaaaagacac
      121 tgagtgacaa tcagatgctc agggtttacc tggttgtgca gaggtttgca gaagcggaag
      181 agcagaacat ggtgaatagt cgtcgacgca atgctatata ccctgcctgg tactggctga
      241 cagttctgga tgttctgcgt tataccggaa tgcgctttaa tcaactgaag catatccgtg
      301 tgagggatgt ctgtccggat gaaagcataa ttgatcttaa gctggaggga agtaaaactc
      361 acagggaatg gggcgttcct ctggtggctc ccctgaaggt atcgatggaa aaactgctgg
      421 agcgggcccg tcagtgcggg gcaggtgatg atgactatct ttttgacgtc tgtcgtttca
      481 gtgagccgat ctctgccgac agatatcagt actgtcccgt tcgggcgcat cagaccgtac
      541 gttcattttt ccggcgactt tcgcgagaat gtggttttcc tgtttcacca catcgtttca
      601 gacatacgct ggcaaccaga ctgatgaaat atcctgaacg taatttacat ctggtcaaat
      661 ccatactggg acacaggagt cttacaacga ccatggggta tgttga
//
LOCUS       DAZKJR010000465          703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKJR010000465 DAZKJR010000000
VERSION     DAZKJR010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.468"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>703
                     /locus_tag="ACHOWS_005501"
     CDS             <1..>703
                     /locus_tag="ACHOWS_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0050893.1"
                     /translation="LPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPL
                     TEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYL
                     LTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTI
                     TDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHL
                     FAHGEYIKGITSLIKY"
BASE COUNT          225 a          134 c          136 g          208 t
ORIGIN      
        1 ctgccgacgt cacagttttg gtcgtacgca acatacttca gggtattgtc ttttgaatat
       61 ctgagtgaaa gtatttctac actgctgtat ctggatgccg atgttgtttg taaaggaagc
      121 ctgaaacctc tcacagaaat tatatttaaa gatgagtttg ctgcggtcat tcctgacaat
      181 gatagtactc aggcggcatg tgcaaaacgc ctcaacattc ccgaaatgaa tggacgttat
      241 ttcaatgcag gcgttatcta tgtcaatctt aaaaaatggc atgaagcaaa tttgacaccg
      301 tatttactca cgcttttacg aggggaaact aaatatggct ctcttaaata tttagatcag
      361 gatgcgttga atatcgcatt taatatgaat aatatctacc tcgcgaagga ttttgatact
      421 atttataccc tgaaaaacga acttcatgat cgtagtcatc gaaagtatca gcaaaccatt
      481 accgataaaa cagtattgat tcactataca gggataacta aaccatggca tagctgggct
      541 ggatatccat ctgcatcata ctttaatatc gcgcgtgaac aatctccctg gaagaaatat
      601 cctcttaaag aggcgcggac tgttgcagaa atgcagaaac aatataagca tctgtttgcc
      661 catggtgagt atattaaagg tataacttca ttaattaagt aca
//
LOCUS       DAZKJR010000466          698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKJR010000466 DAZKJR010000000
VERSION     DAZKJR010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.469"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(222..>698)
                     /locus_tag="ACHOWS_005502"
     CDS             complement(222..>698)
                     /locus_tag="ACHOWS_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFQ0050894.1"
                     /translation="IGLKGGWNFYQYPLNPVSGFDPLGLKVSFQGDESTQKTLKEAYK
                     AVAETKFGHKITEELESSEHEYIFRGLRKGINQTCYDDTEYSFYIDIDNDHSSCVYQG
                     KNKACAMKPTLLSVVLAHEMGHAKGMKDDGTDSMANVDKYENPFRKELGLPARMKY"
BASE COUNT          215 a          143 c           96 g          244 t
ORIGIN      
        1 atctgtaata atttttgtgc accatctttt attgcaaaat tttcaatttt acttttagtg
       61 acaggggaat atgtttggat gcgaaaaggc atcctataat ggaaaacatc agctatacag
      121 caagatgaaa aaaatacgga aataaaatac agacatactt tcataccatc tcctaaactg
      181 gtgatatatt aaaaacatac ccaaaagcaa tataaaaatg tttaatattt cattcttgct
      241 ggcaagccca actctttacg aaatgggttt tcatatttat caacattagc catcgagtca
      301 gttccatcgt ctttcattcc ctttgcatgc cccatttcat gcgcaagaac cacactcaac
      361 agagtgggtt tcatggcaca tgctttgttt tttccttgat atacacatga gctatggtcg
      421 ttatcaatat caatgtaaaa actatattca gtatcatcgt agcatgtttg gtttatgcct
      481 tttcttaatc ctctaaatat atattcatgc tctgatgact ctaattcttc tgttatttta
      541 tgtccaaact ttgtttctgc tacagcttta tatgcttctt ttaaagtttt ttgtgtactt
      601 tcatctccct gaaaagacac ttttaaccct aatggatcga aaccagatac cggattcaac
      661 ggatactgat aaaaattcca tccccccttc agcccaat
//
LOCUS       DAZKJR010000467          696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKJR010000467 DAZKJR010000000
VERSION     DAZKJR010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.470"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..450
                     /locus_tag="ACHOWS_005503"
     CDS             <1..450
                     /locus_tag="ACHOWS_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFQ0050895.1"
                     /translation="CGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQEQNIREKTDAV
                     FRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRSQAR
                     EQFTGAVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          151 a          196 c          184 g          165 t
ORIGIN      
        1 tgcggacatc agcaggtgct gtttgtggtg acgcaggccg acaaaacgga gccctgccat
       61 gaatgggata tggccggcat tcagccttct cccgcacagg aacagaacat tcgtgagaaa
      121 acagatgcgg tattccggct gttccggccc gtacatccgg ttgtggccgt atcggcccgc
      181 accggctggg aactggatac gctggtcagt gcgctcatga cagcacttcc cgaccatgcc
      241 gccagtcccc tgatgacccg cctgcaggac gagctgcgca cggagtctgt ccgctcgcag
      301 gcccgtgaac agtttaccgg tgcggtggac cggatatttg acacagcgga gagcgtctgt
      361 gttgcctctg ttgtccgtac ggccctgcgc gctgttcgtg acaccgtggt ctctgttgcc
      421 cgcgcggtat ggaactggat cttcttctga acctgttgtg gatgatgtcc tccctgcctc
      481 tgagtctgct cacaaaagcg ctgttttcgt tactgtctct cttgtccgtg caatagccaa
      541 ataatagaat aaaacgatcg atacctattt tatcgatcgt ttatatcgat cgataagcta
      601 ataataacct ttgtcagtaa catgctcaga tacgtacaga aagacattca gggaacaaca
      661 gagccacacg tcagaaactt ccgtcagccg gacctc
//
LOCUS       DAZKJR010000468          686 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKJR010000468 DAZKJR010000000
VERSION     DAZKJR010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.471"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          281 a          148 c           97 g          160 t
ORIGIN      
        1 taaaagagat aaccagcagc acagacaacc tgataaaact gttttgtatt acctcattac
       61 agcaatgagg taatatttca gaatacagtg agactctctt aaactgagga ccagtacaaa
      121 ttacaatggt aaaaacacca cctcatagaa ttgagtttaa gaaaacacca ttcactcaac
      181 ctgacattca caaaagtcag taccaggtag cctcaggtaa catcgctatt aatattatcc
      241 tgccattaag taaaaacaga aactctgtga ggaagcctca ccaatatttg accagtagca
      301 taaagaataa cgatatgaga gatacgccca caccagagat gattagccca tacaaaaaga
      361 taatatttat taataccacc gaacctcaat agagtaataa cacactaata gcgttttagt
      421 acacacttaa cactaactat gcaccagaca catactcagg attaacattc tcaacattta
      481 tggtactctc aaagagtata attcaccaca caggtaaact ctcacaccag tagtatggag
      541 ataatgctgt acacatactt acagagtaaa gtgtacagct agcatacagt ggcgagcaga
      601 cattacaaca taaagactca agatagcata ataaagcata ataagaacac aacaaaactc
      661 ccactaataa tactaattaa ccttaa
//
LOCUS       DAZKJR010000469          685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKJR010000469 DAZKJR010000000
VERSION     DAZKJR010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.472"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..553
                     /gene="ydeO"
                     /locus_tag="ACHOWS_005504"
     CDS             <1..553
                     /gene="ydeO"
                     /locus_tag="ACHOWS_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFQ0050896.1"
                     /translation="TVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSC
                     LSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLK
                     QEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRV
                     SKEYRCQRHTGMNTDNTMSALAI"
BASE COUNT          208 a          132 c          141 g          204 t
ORIGIN      
        1 gacggtgcct gttcctgcga agtttttacg tgcttcaaat gtaccgactg gattgcttaa
       61 tgaaatgatt gcttatctca actcggaaga acgcaatcat cataattttt cagaactttt
      121 gcttttttct tgcctgtcta tttttgccac atgcaaaggt ttcattacac tattaactaa
      181 cggtgtgcta tccgtttccg ggaaagtgag aaatattgtc aacatgaagc tggcgcaccc
      241 atggaagctg aaagatattt gtgactgcct gtacattagt gaaagcctgt tgaagaaaaa
      301 actaaagcaa gagcaaacga cattctcaca gattctttta gatgccagaa tgcagcacgc
      361 aaaaaatttg atacgcgtag aaggttcagt caataaaatt gccgaacaat gtggttatgc
      421 gagtacatct tattttattt atgcgttccg caaacatttc ggcaacagtc cgaagagagt
      481 ttctaaggag taccgttgtc aaaggcacac gggtatgaac acggataaca cgatgagtgc
      541 tttagctatt tgattatttg ctaacgagta gtcaaccacg cacgttacgt agaaattaaa
      601 atggggcagc cattccctgc cccgcgttgt ttttaggcga tatattattg aaataaataa
      661 gtgacattca tcacatattt atgca
//
LOCUS       DAZKJR010000470          679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKJR010000470 DAZKJR010000000
VERSION     DAZKJR010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.473"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>679
                     /locus_tag="ACHOWS_005505"
     CDS             <1..>679
                     /locus_tag="ACHOWS_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HFQ0050897.1"
                     /translation="GSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRE
                     TGRESAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQL
                     PSLDSSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLW
                     QGLSHAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEE
                     DVMYGDEA"
BASE COUNT          128 a          190 c          218 g          143 t
ORIGIN      
        1 ggatccgccg tggcagactg gccggaagcg gagaagccgt cttttgcggg gctttgttcc
       61 gcgcttgaga accgcctggc acgttcaggg ggcatggagg cactggtccc ccagaacagc
      121 agtccccggg agaccgggcg tgaatcagct cccccggact caccacaact ggcagcggtg
      181 acctcggggc gcgacttact cgaccaggcc aggttacttt cccgctggct gagcgggcag
      241 ccgcagggct ggctggcttc tcaccggctg atgaagacgg tgcggtggga tacggtcgat
      301 cagctccctt cgctggacag tagcggacgc acccgcctgg tccccccgaa aaccgggtat
      361 cgcgctcagc ttaagcgact ctggcttcag aaaaactggg ttgagctgat tgaactggcc
      421 agtcagatgt attgcgaagg tgtgaatcat ttctggctcg atcttcagtg gtatctctgg
      481 cagggactca gtcatgctgg tcagccctgg gattcctggt ctgacgctgt cctgtcagat
      541 ctgcggctgt tgcttaaacg tctcccgggc cttgaaggac tggcatggaa tgacggtact
      601 ccctttgctg acgaggtcac cctcgcctgg atcgctgaaa aagtgaatga agaagatgtg
      661 atgtacggcg atgaagctg
//
LOCUS       DAZKJR010000471          679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKJR010000471 DAZKJR010000000
VERSION     DAZKJR010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.474"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..611)
                     /locus_tag="ACHOWS_005506"
     CDS             complement(<1..611)
                     /locus_tag="ACHOWS_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFQ0050898.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKANCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDG"
BASE COUNT          191 a          158 c          131 g          199 t
ORIGIN      
        1 tccccatcac tataacttgt tgatgtatat gcctggttat tattaaaagc atcatcactt
       61 aaatagacgt cattattgat ggtataaatt gcaccgcctt tgccgtcatt agcgatgtta
      121 ttgtgaaata cggcgttagt gacacgtaaa tcggcggcac cggtatcatt ggtaccggca
      181 gaatagattg ctccgccata gcctcctgca acattactgt cgaaaataac attcgcaaga
      241 tttatcgttg agttttcttt agcaaagata gccccaccat tattatattc gccactaacc
      301 gagttatcag caaacagggt catccctgtt tcattttgtg gtgaaatcgt aaattcagca
      361 ctttgctgca aaaatactgc gccaccgctg gcattattgg ccatacccga aaaaatcaac
      421 cattgcccat ccgcgatgct ccagtcctgc atattgcccg aaaggctttg cctgctggcc
      481 tgacagttcg ctttgatatc atatccctgg catgaatcga tgaccgctgc ggcaccgtta
      541 ccagagaaaa gagatgcaat cattgacggg agtaaagata aatactcctt gcgtagaaag
      601 ataatccgca tattcatcta accatcattt tctataagat ggcgtattta atcgcattat
      661 gcgatatagg gtatttttc
//
LOCUS       DAZKJR010000472          674 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKJR010000472 DAZKJR010000000
VERSION     DAZKJR010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.475"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..568)
                     /gene="fimE"
                     /locus_tag="ACHOWS_005507"
     CDS             complement(<1..568)
                     /gene="fimE"
                     /locus_tag="ACHOWS_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFQ0050899.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLI"
BASE COUNT          202 a          170 c          139 g          163 t
ORIGIN      
        1 ttatgagatt atttctttcc cataatccgg caaaacgagc agcattactg gcggtataac
       61 gtacagtatg gcgaatattt cgatgcccga gataatcctg aattaaacgg gtatcggcac
      121 cacgctccgc cagttcataa ccgcaagcat gccttaacat atgaggatga gtctgcgtta
      181 cggttccagc ttcaatacct gcatcgcgaa taatgcgata ggcctgctgg cgagaaagcc
      241 gactcccgcg gcgagaaata aatatagcgt cggtccggtc agcgcctttc cagttagcac
      301 gttcctgggt ccagcgttcc acggcttcac gctcatcaaa acgtaacggg tgaacggtag
      361 aaaatccgtt cttcagtcgg cgaatattta ttcgaccttc attaaggtca agatcctgat
      421 aatgcagatc aagcagttca ctaatacgca tcccatgccg atatgccaac agaataagac
      481 aataatctct ggctcccgtt gccccgtaac aaaccgcctg catcatggcc tgaacttctt
      541 taccggtaag ataacgacgt ttactcacaa tagtagtact cctaactgag atatatttga
      601 atgaatacct ataggaaacc tcaatcggtc aaaattagcc caatagataa aaagataacc
      661 acacacgaag tgat
//
LOCUS       DAZKJR010000473          671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKJR010000473 DAZKJR010000000
VERSION     DAZKJR010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.476"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHOWS_005508"
     CDS             complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHOWS_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFQ0050900.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          157 a          187 c          125 g          202 t
ORIGIN      
        1 ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca
       61 cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat
      121 agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct
      181 gtatcttcgc cgtgttctta cccaccgtat cgacgtagta ccctctgcac cagaactctc
      241 tgttcctgta tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc
      301 ctttcagata tcccataaag ccggatacac tcattttggg cgggatctcc acaagcatat
      361 ggatatgatc tgcacagcat tcagcttcaa gaatccgtac gcttttccac tcacacagct
      421 ttctcaaaat actgcctatt gctctacgct tctctctgta gaacacttgt cttcggtatt
      481 ttggcgcaaa aactatgtga tatttacagt tccatcgggt gtgcgctaag ctcttttcgt
      541 tccccattgg gacccccttt tgatttcttg tttgacactt gcagttgcca gaccgcaagg
      601 tgttttaaca aatcaaaagg ggttttaata actggctcaa agctgaaagc tttccggaac
      661 ccccagccta g
//
LOCUS       DAZKJR010000474          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKJR010000474 DAZKJR010000000
VERSION     DAZKJR010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.477"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..602)
                     /locus_tag="ACHOWS_005509"
     CDS             complement(<1..602)
                     /locus_tag="ACHOWS_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFQ0050901.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          166 a          167 c          159 g          178 t
ORIGIN      
        1 ttttcgtcgc aggcttcaat catgcgtttc gggtccataa acaactgacc ggggcgcata
       61 gggatctcac gcaactccac atcccagtag cgggcgaatt tatgccagca gatttgtacc
      121 ggaccgcaca ccaggtttgg tttatccgtt ggtttgcctg cggcttccat acgcttgcgc
      181 caacgccatt tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg
      241 gtgccaacgg cctgaccatt tttcggtgca ggcgcatgcc acagatcggc aaccatgttt
      301 acgcaacgca ggtcgattgc agcggattgc ggatattctt ctttgtcgat ccagttttta
      361 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
      421 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctgaaatgca
      481 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      541 tttgcaccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      601 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      661 agcaaggcat
//
LOCUS       DAZKJR010000475          663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKJR010000475 DAZKJR010000000
VERSION     DAZKJR010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.478"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..>663
                     /locus_tag="ACHOWS_005510"
     CDS             101..>663
                     /locus_tag="ACHOWS_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050902.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASPAPGHLKDKFIEPLVKRVKDVAEGR"
BASE COUNT          198 a          110 c          188 g          167 t
ORIGIN      
        1 gctgaatgaa taaaatcttc atctggtgtt tttgttgcgc gtaaatgcag gtgtaactgt
       61 ggatgttatc cactgaagag ctgctgatga ggatgaagac atgctgacta aaaaaatgtt
      121 actcgtttgt gcttcgtgtg ttgcgctgag tgcctgttct gcacaggaaa ttgcagagct
      181 gaacaggcag ataagtgacg gtgcatttgc tgtgacaaaa gtactgaacg taggtgtaaa
      241 ccaggcagat gatgatctca aatatctgtc tcaggcaccc cgggcaaaaa gagagaaacg
      301 tgatcatgtc atagaagtcc ctgttgatgt ggatacagct gcgggaagac tgaaacggta
      361 ctataagttt tattcaaatg aagaagtcga tgcgattcgt aataacggac atgcgaacgg
      421 gaagtgggcc gcagcagcaa taagtcagaa cgggcatatg tgggatgcca tgcccggtag
      481 ttattataaa atgggcagtg actgggatgg tggcgagttt gatgatcatc tgacgcttga
      541 acttgaaaaa aatggcaaag gaagtcggat atatataacg tatgcatctc cggcaccggg
      601 gcatctgaaa gataaattta ttgagccatt agttaaacgt gtgaaggatg tggctgaagg
      661 ccg
//
LOCUS       DAZKJR010000476          661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKJR010000476 DAZKJR010000000
VERSION     DAZKJR010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.479"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..630
                     /gene="pagP"
                     /locus_tag="ACHOWS_005511"
     CDS             70..630
                     /gene="pagP"
                     /locus_tag="ACHOWS_005511"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFQ0050903.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYDP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          179 a          125 c          158 g          199 t
ORIGIN      
        1 ttaaggttat gttaattgta gctttgctat gctagtagta gatttttgat aaatgtttta
       61 tggtcacaaa tgaacgtgag taaatatgtc gctatctttt cctttgtttt tattcagtta
      121 atcagcgttg gtaaagtttt tgctaacgca gatgagtgga tgacaacgtt tagagaaaat
      181 attgcacaaa cctggcaaca gcctgaacat tatgatttat atattcctgc catcacctgg
      241 catgcacgtt tcgcttacga caaagaaaaa accgatcgct ataacgagcg accgtggggt
      301 ggcggttttg gtcagtcgcg ttgggatgaa aaaggaaact ggcatggcct gtatgccatg
      361 gcatttaagg actcgtggaa caaatgggaa ccgattgccg gatacggatg ggaaagtacc
      421 tggcgaccgc tggcggatga aaattttcat ttaggactgg gattcaccgc tggcgtaacg
      481 gcacgcgata actggaatta catccctctc ccggttctac tgccattggc ctctgtgggt
      541 tatgatccag tgacttttca gatgacctac attccgggta cctataacaa tggcaatgtg
      601 tactttgcct ggatgcgctt tcagttttga gacaaatgaa attttagtaa actctttaaa
      661 a
//
LOCUS       DAZKJR010000477          653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKJR010000477 DAZKJR010000000
VERSION     DAZKJR010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.480"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>653
                     /locus_tag="ACHOWS_005512"
     CDS             <1..>653
                     /locus_tag="ACHOWS_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFQ0050904.1"
                     /translation="ELLREDGVIFISIDENELNQLKTICDEIFGEANFIENIVWNKRI
                     PKNDKGIGNIHEYILAYAKNNEISKEFLMRKDGIDDVYQFIEKLKKEKVPLDKAEQQL
                     KKFYSSNGYDRGITLYNGLNEDYRPWGKINMSWPNADSFGPTYEVLHPLTNNPVKIPD
                     RGWRWKEGTFNHIAKRIDGKYADIKKLHDGSVICGGIWFARTEKTQPSSVKFLDEVE"
BASE COUNT          238 a           89 c          138 g          188 t
ORIGIN      
        1 gtgagcttct acgtgaagat ggtgtgatat ttatttcgat tgacgaaaat gaattaaatc
       61 aacttaaaac tatttgtgat gaaatttttg gcgaagcaaa tttcatagaa aatattgtat
      121 ggaataaacg tatccctaaa aatgataagg gtattggtaa tatccatgag tacatccttg
      181 cttatgcgaa gaataacgaa ataagtaaag aatttttaat gcggaaagat ggtattgatg
      241 atgtatatca gtttattgaa aagttaaaaa aggaaaaggt gccattagat aaagcagagc
      301 agcaactcaa aaaattctac agctccaacg gatatgatcg tggaattact ctttacaacg
      361 gtttgaacga ggattatagg ccatggggta aaattaatat gagttggcct aatgcagatt
      421 catttggacc cacgtatgaa gtattgcacc ctttgactaa taaccctgtc aaaatcccag
      481 acagaggctg gcgttggaaa gaaggcactt ttaatcatat tgcaaaaaga atagatggaa
      541 agtatgcaga tatcaaaaaa ctacatgatg gtagcgttat ttgtggtgga atttggtttg
      601 cccgtacaga aaaaacccaa cccagttcgg ttaaattttt agatgaagtt gaa
//
LOCUS       DAZKJR010000478          633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKJR010000478 DAZKJR010000000
VERSION     DAZKJR010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.481"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>633
                     /locus_tag="ACHOWS_005513"
     CDS             <1..>633
                     /locus_tag="ACHOWS_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050905.1"
                     /translation="MEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGD
                     LQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALP
                     FSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGI
                     WSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKL"
BASE COUNT          201 a          123 c          130 g          179 t
ORIGIN      
        1 catggaatac ctggtaactt gtcagttgga tgaagaacaa atgtcatcgc tgctttatga
       61 aagagatgat ttaagcgcca ttgatttttc attgctcatg aagacgataa aagccttttc
      121 atttggtggc gatctccaaa cccttgcatc aaaaccgggt tcaacaatct caagcatccc
      181 gtcaaaaaga cggatattga ttaacattaa ccacgatttt cctaacaatg gcaatctttt
      241 caatgacttt ctatttaacc atcaacaaga tgaacaatta gcaatggcat atatggccgc
      301 tctcccgttc agcaggcctt tagtttactg ggatggccaa gtattaaaat caacgactga
      361 aattaaaaat tatgatgggt cgacgcgtgt cggcggtgag gcgtggctta ataaaggttg
      421 ctctacctat cagcagctct acaatgaatt ccacgcatta tatatagata aagcaggaat
      481 atggagcgca tttgagggtg tatttgcaac taaaaacgtt ctggccttta gtcgtgggga
      541 ttctgtgaac attaatcact ctcctcatga tggactagtt ataataaata aaggaaacga
      601 agaagttgaa ggtacctggc ctaacaaatt gca
//
LOCUS       DAZKJR010000479          631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKJR010000479 DAZKJR010000000
VERSION     DAZKJR010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.482"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..123
                     /locus_tag="ACHOWS_005514"
                     /pseudo
     CDS             61..123
                     /locus_tag="ACHOWS_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
     gene            420..613
                     /locus_tag="ACHOWS_005515"
                     /pseudo
     CDS             420..613
                     /locus_tag="ACHOWS_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          182 a          144 c          162 g          143 t
ORIGIN      
        1 tgactgcctc ggcatgccag ttaacagcca tccgcaatgg cgtgttcggt tttgcgaata
       61 aaaaatggtt actgcagaag cggattttca ccacctgtca accatgaatg gtcagtggag
      121 tgagacgact ggtatgattt ggtcgatgac ttcttccggt gaatacggac agaaacattg
      181 aaatattgta agagagaacg ttcagaattc tgtgtcacgt gaaaatacta cagcgttatc
      241 accttattca gtcgaccttc aaaataaacg tctaaccgtg acaatcacca tttggattgc
      301 ccactgggga ggcgaacgat tgcctcgacg gcatttataa tgccagccca cattgttggc
      361 gctgacaata ctaaggacaa acgatgaggg agaaccgtct tataccacta gcgctgacac
      421 cggcacacct tccccgtgaa atacaccggc tggagcctga agaaagctgt tgcccggagt
      481 gtggcggtga gctggattat ctggggaagt cagcgctgaa caactggaac tggtgagcag
      541 ctccctgaaa gtgatcagca cagaacgggt aaaaaaagcc tgtaaaaaat gtgacggtac
      601 cgttgaagcg ccggtctcgt aagaataatc c
//
LOCUS       DAZKJR010000480          630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKJR010000480 DAZKJR010000000
VERSION     DAZKJR010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.483"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..233
                     /locus_tag="ACHOWS_005516"
     CDS             6..233
                     /locus_tag="ACHOWS_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0050906.1"
                     /translation="MEIENTPENIKRLRKKIGLTQKECAEMFGMTARSWRRKEEPTGT
                     VSGSKLSPVEFEYILLLAGEHPEYVLSKRDA"
     gene            344..630
                     /locus_tag="ACHOWS_005517"
                     /pseudo
     CDS             344..630
                     /locus_tag="ACHOWS_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866645.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          211 a          101 c          146 g          172 t
ORIGIN      
        1 tgaaaatgga aattgaaaat acaccagaaa atatcaagcg tctgcgaaag aaaattggat
       61 taacgcagaa agaatgtgca gagatgttcg ggatgacagc ccgttcatgg cggcgcaaag
      121 aagaacccac tggaacggta agcggatcaa aactttcacc tgtagagttt gaatacattc
      181 tgttactggc tggggagcat cctgaatacg tcctttctaa acgggatgcc tagcaaaaaa
      241 gaagaaaaat aacttgcaaa taggtcgttt atgacctatt attaatatat cgaaagggga
      301 tggtccccaa taaccaaagc cgcacagtgg caggagatta aaaatgttaa cttttaaaaa
      361 tggtacagtt tgtgttggtg acatatttgt gtcttcatgg ggatacgaac aaacgaatgt
      421 taatttctat caggtcattt ctgttcacgg aaaaaaacgg tgactgttcg tgagatttgt
      481 gccagtgttc actttaccgg aaatatgatg gggtataaac gtccgttgtt gaatgatttc
      541 agaggagagc cactaaaacg tcgagttctt gattgttatc gtgttccagt aattgtaatt
      601 gaagaattcg aaatagcgta taaaacttta
//
LOCUS       DAZKJR010000481          621 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKJR010000481 DAZKJR010000000
VERSION     DAZKJR010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.484"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..559)
                     /locus_tag="ACHOWS_005518"
     CDS             complement(<1..559)
                     /locus_tag="ACHOWS_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFQ0050907.1"
                     /translation="MNIRNNHLPGTETLFSETETANSKQLAVLKTNFPQCFDKNGAFI
                     QEKLLEIIRASNVEISKESYCLNWLGKSYARLLANLPPTTLLSEDKDHNRREENKNSQ
                     NLLIKGDNLEVLKHMVNAYSEKVKMIYIDPPYNTGSDGFAYNDDRKFTPEQLSDLAGI
                     DLDEAKRILEFTTNGSSSHSAWLTFI"
BASE COUNT          172 a          121 c          131 g          197 t
ORIGIN      
        1 aaatgaacgt taaccatgcg ctatggctgc ttgaaccatt cgtggtaaat tcaagtatac
       61 gtttagcctc atcaagatcg atacctgcca gatcagaaag ctgttctggt gtaaatttgc
      121 gatcatcatt ataggcgaac ccatcagaac ccgtgttata tggcggatcg atataaatca
      181 ttttaacctt ttcagaataa gcattaacca tatgctttaa tacctcaagg ttatcccctt
      241 tgattaacag attttgactg ttcttgttct cttcacgtct gttatggtct ttatcttctg
      301 acagcaatgt cgttggtggt aggttagcca gcaggcgagc ataggatttg ccaagccagt
      361 ttaagcagta tgactcttta gagatttcaa catttgatgc tctgatgatc tcaagcagct
      421 tttcctgaat gaaagctccg tttttatcga aacactgtgg aaagttagtt ttcaaaacag
      481 caagttgttt actgttcgca gtttcagttt cagaaaaaag agtttctgtt cccggtagat
      541 gattgttgcg tatattcata cagcacctcc agaaagcacg gaggtagagg tacttgacgg
      601 agagagttca ggcgagcata t
//
LOCUS       DAZKJR010000482          621 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKJR010000482 DAZKJR010000000
VERSION     DAZKJR010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.485"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..>621
                     /locus_tag="ACHOWS_005519"
     CDS             56..>621
                     /locus_tag="ACHOWS_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFQ0050908.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLL"
BASE COUNT          144 a          148 c          178 g          151 t
ORIGIN      
        1 cctcacggga caatgccctc tttttacatc tgtataaaaa agaggagcgt aaacaatgtc
       61 tgaatggtgc aaaaaccgtt ttgaaattac cggtaaatca gtctgtcttg atgtacttac
      121 acagtggatt gaagggcgtg agaccccgcg ctaccgtcat gccatacagc agagtataca
      181 gctgtttctg gccggttgtg cagggctggt aaaacccaca cggacgacgc agtatccgcc
      241 atatccggcg ctggttcgtc acggaacggg gttgtcatct ccggctaacc aggcgtttga
      301 gcagtggctg gggttgctgg taaaaaatgc cgttctggat ggtgaaacga taaagaccat
      361 tgagcgtctt tatcaccagt catgtatcgg cgctgttcgc tggggtaatc tgccggataa
      421 tgccagggag atcatcacca cgctgatgag ccgtcagtat tctgactggt ttggtctggc
      481 cgggctgagt gaacacattg atgcagaagc ctgctggagc tggttgtcgg attacccgga
      541 acaggcgcag ccctgtgaca tgctgatggt cataccctcc cgtctggcta cagagctgaa
      601 tggttcaggt ggactgctgc a
//
LOCUS       DAZKJR010000483          616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKJR010000483 DAZKJR010000000
VERSION     DAZKJR010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.486"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>616
                     /locus_tag="ACHOWS_005520"
     CDS             <1..>616
                     /locus_tag="ACHOWS_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFQ0050909.1"
                     /translation="GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVE
                     KSGSGTLTVSNTTLIQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAID
                     PTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNG
                     TISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLAT"
BASE COUNT          160 a          188 c          168 g          100 t
ORIGIN      
        1 gggcaccacc acgctgaata acggtgccac gcttaccctt tccgggaaaa cggtgaataa
       61 cgataccctg accatccatg aaggtgatgc actcctgcag ggaggcgctc ttaccggtaa
      121 cggcagggtg gaaaaatcag gaagtggcac actcactgtc agcaacacca cactcatcca
      181 gaaaaccgtc aacctgaatg aaggcacgct gacgctgaac gacagtaccg tcaccacgga
      241 tgtcatcgct cagcgcggca cggccctgaa gctgaccggc agcacagtgc tgaacggtgc
      301 cattgacccc acgaatgtca ccctgacatc cggtgccacc tggaatatcc ccgataacgc
      361 cacggttcag tcggtggtgg atgacctcag ccatgccgga cagattcatt tcacctccgc
      421 ccgcacaggg aagttcgttc cgacaaccct gcaggtgaaa aacctgaacg ggcagaatgg
      481 caccatcagc ctgcgtgtac gcccggatat ggcgcagaac aatgctgaca gactggtcat
      541 tgacggtggc agggcaaccg gaaaaaccat cctgaatctg gtgaacgccg gcaacagtgg
      601 tacggggctg gcgacc
//
LOCUS       DAZKJR010000484          614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKJR010000484 DAZKJR010000000
VERSION     DAZKJR010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.487"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..492
                     /locus_tag="ACHOWS_005521"
     CDS             <1..492
                     /locus_tag="ACHOWS_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFQ0050910.1"
                     /translation="DYSQPMADVYIRGDITAPQSCEINSLRPINFDFKEIPAADFSSV
                     VGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPSTDNSMVVTSNPNVGIKIYD
                     KNNKEINVNGGELPTDMGKSTVFGEKAGSVTFSAAPASLTGARPAPGTFTATATITIE
                     IVR"
BASE COUNT          189 a          131 c          119 g          175 t
ORIGIN      
        1 gattactcac aacctatggc ggatgtttat attcgtggcg atatcactgc accgcaaagc
       61 tgtgaaatta acagtttaag acccattaat tttgatttta aagaaattcc tgcggcagat
      121 ttttcttcgg tagttggaag taccgtgaca acgcataaaa ttaccaaaac cgtcaccatt
      181 gagtgtgaaa atttaggaat actaaatact gatgatatca gtacctcttt ttatgctacc
      241 gaacccagta ctgacaactc aatggtcgtg acatcaaacc cgaacgttgg gattaaaatt
      301 tatgataaaa ataataagga aatcaatgtt aacggtggtg aactgccgac agatatgggt
      361 aaatcaaccg tctttggtga aaaagccggt agcgtcactt tttctgctgc tcctgcaagc
      421 cttacggggg ctcgccccgc gccaggaaca tttaccgcga ctgcaacgat aacaattgag
      481 attgtacgct aaacgaactg acagcggtgt tcctcacacc gttgtcatct tacgcatttt
      541 tgtccgcgag ttttcactct gcatgacaaa aagatgacat tacttttctt tttcttagtc
      601 aataaaatca aaaa
//
LOCUS       DAZKJR010000485          609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKJR010000485 DAZKJR010000000
VERSION     DAZKJR010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.488"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..262)
                     /locus_tag="ACHOWS_005522"
     CDS             complement(<1..262)
                     /locus_tag="ACHOWS_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFQ0050911.1"
                     /translation="MKTSDNESTKYAITGQAVLQILRMKINFSLQTLIKQLLVMKSVE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAITDNKSIFNGKKIN"
     gene            complement(305..541)
                     /gene="ycgZ"
                     /locus_tag="ACHOWS_005523"
     CDS             complement(305..541)
                     /gene="ycgZ"
                     /locus_tag="ACHOWS_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFQ0050912.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRIL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          172 a          128 c          103 g          206 t
ORIGIN      
        1 tgtttatttt cttaccattg aaaatacttt tattgtcagt tatcgctgtt cttctgtttg
       61 gccctcctgt atcactgttg ctaaaatcac gaattatgct gtcaattaaa tctcgtcgga
      121 aagcattttc ctcaacagat ttcattacga gtaattgctt aataagagtc tgcaacgaaa
      181 aatttatctt catgcgcagt atctggagca ccgcctggcc agtaattgca tattttgtac
      241 tttcattatc agatgtcttc ataacgactt catagctaac ctgttaaata atcaggctgt
      301 tgcattattc aaaaagcaac ccaattagtg cattataatg tttctgttcc tcttcccctg
      361 tcgcttgttc cagtcggcat agaagtttgg cacaaagcga ctttcgactc agaatacgtc
      421 catctttcaa aatctcagtc acaatttccc cgagagtttc ctgctgagta ggcaagttag
      481 cttttgcaaa gtaacgagtg attgctcccg cagaatctaa agtcactgaa ttttgatgca
      541 tgctacgcct ctgttaaaaa tgttaatcaa tgttaagtga ggtaacaata gcaaacttgt
      601 acgaaatat
//
LOCUS       DAZKJR010000486          608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZKJR010000486 DAZKJR010000000
VERSION     DAZKJR010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.489"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          174 a          138 c          143 g          153 t
ORIGIN      
        1 ctcaacaaaa acctggcagt tgctaaaatc gacttctgta ttaaacgtat ccggatgcat
       61 gaacaaatga gcaggataat ccggaagact ggcacagacg aaatactctt ccgtctgagt
      121 aaacaccatc atttatatga gaaaaagaaa acaaaagagg ggtgagaaac ccattgtatg
      181 gggaaaactt gccatgctca ctgcgggggc atttttgtac tggctgttct aaccgggtaa
      241 cccgttgata cacatagtac aataatgttt tatgcaggct tacgcgccag actgccatca
      301 gtagtgcatt gcgtgccagc tcgcggacag gggcagtgac cagccaccac atgagtaagc
      361 atcatttcag cgccatcagg cagctcctga aactcctgcg agttgaaaaa gcatcctcct
      421 gcattagcaa tactggcttg tcagtgccag tgtgatataa gacgtaatgt caaagttcgt
      481 ggtgttgaca tatttctgtc gcaaccaaag tatgctcccg cccagtatat atctgggcag
      541 gagcatcatt tattagttct ttttcctgtc ggggccaaaa cattctgtcg atgtggcgca
      601 ggttgata
//
LOCUS       DAZKJR010000487          607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZKJR010000487 DAZKJR010000000
VERSION     DAZKJR010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.490"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(238..>607)
                     /locus_tag="ACHOWS_005524"
     CDS             complement(238..>607)
                     /locus_tag="ACHOWS_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFQ0050913.1"
                     /translation="LKKLCIAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIR
                     NHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMDRNYLASVLAGSGLS"
BASE COUNT          159 a          136 c          112 g          200 t
ORIGIN      
        1 atcttgatag tccatgccag cattataata atcacaagat ataattatca tggaaataat
       61 tatcactaat atcttcccaa gcatttccat ttactttacc tcttgaaatt caaatgggaa
      121 tccagtgaaa ggacctttag gcataatata tctttgatat ccataaagct caccgagcac
      181 ttgtaggctt ataggcgcat ttgtattcat agaggggttg ttaaacccag ggaaagatta
      241 cgaaagcccg ctccctgcaa ggactgacgc caggtagttt ctgtccatgg ctgcttttcg
      301 catcttacgt cttaaccctg ccttgaatac cttatcattc gttaaaatat taatagcgat
      361 gtgccgtatc cctgaaaata attctgcggc gtttcctctt cttattttgc agtcgtcttc
      421 attcattacc acgtccagac gccagtgcag cttattctcc acgtgccagt ggtttcggat
      481 tgctgtggcg aacttttctg cggttaaatc agcagaactg atataatatc tgactgtcat
      541 ttcgggctct ttcttttgct ctgctattat tgagcgaaag gagactgcta tgcataattt
      601 cttcagc
//
LOCUS       DAZKJR010000488          604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZKJR010000488 DAZKJR010000000
VERSION     DAZKJR010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.491"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..269
                     /locus_tag="ACHOWS_005525"
     CDS             <1..269
                     /locus_tag="ACHOWS_005525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050914.1"
                     /translation="IYPAAEGIDMNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPD
                     FTPAQDKPQNMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            complement(320..>604)
                     /locus_tag="ACHOWS_005526"
     CDS             complement(320..>604)
                     /locus_tag="ACHOWS_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0050915.1"
                     /translation="FWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVM
                     GTMFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
BASE COUNT          178 a          160 c          105 g          161 t
ORIGIN      
        1 ttatttatcc tgctgctgag ggtatagata tgaataacga atctgaaaaa gcaatgtggc
       61 gttgtaagcc tgtggataat ccatggacct tcacttccct gctgaaaaaa gcagtctgtg
      121 ccgttttacc agatttcaca cctgctcaag ataaacccca aaatatgttt tcactaagta
      181 atgaaaaaga tactgattat tcagtaccaa tgagaccctt tgcattatat gacccgtgga
      241 aacatcacca tgacaatgct gatttctaag aaaagcaagg gtttccccaa ttatcccagc
      301 taaggaaacc ctaagaattt tacttattag ctaaatctcc actttttgct gctgccttag
      361 atacattact tgttcctttg ttaattgcat cccccataac cgttccgaca cttaccccag
      421 cccaggacaa cgctcccagc cacactgccg gcaagacgag aaacatcgtc cccatcacca
      481 gattcattat cagatcatcc gatgtgttct ggaatcccat catattgaac cgggtatgtg
      541 tatccgaact gtacagcgcc gtcagcatcc agctgtcaag ccaccgtgca agctcccacc
      601 agaa
//
LOCUS       DAZKJR010000489          572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZKJR010000489 DAZKJR010000000
VERSION     DAZKJR010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.492"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..374
                     /locus_tag="ACHOWS_005527"
     CDS             117..374
                     /locus_tag="ACHOWS_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFQ0050916.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            377..>572
                     /locus_tag="ACHOWS_005528"
     CDS             377..>572
                     /locus_tag="ACHOWS_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050917.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGL"
BASE COUNT          150 a          128 c          145 g          149 t
ORIGIN      
        1 cccggaaaca ttcataataa aaatccagac aatcactatc gatggcagag cccagcataa
       61 acatgggaat taaagagtga tgaattagtt aagagataag aaagaggaat ttaataatgc
      121 ttcatattat tcgtaaaggc gataaaacta ctcacggagg ttccgttctg accgcttctg
      181 aaacgatgaa attcggtggg ataggcgtcg cccgtaaagg cgataaagtg tcatgtccgg
      241 gtcatggtcc taccatcatc atagaaggca atcctgatta tcttgaccat ggtatcccgg
      301 tggcatttca cggtcataaa tgcgcctgtg gttgcacatt gatcagttca tttgccgcag
      361 caaaggttgc ctgattatgc ctgtctggct ggatgccatc cctgaaaagg ccccaaagat
      421 agcgcgcccc aatactcgcc gctggctgtt gttcctggca tttatcatgc tgggggggat
      481 ggcgctcaca ttatggtgct ggacgtcaga acgaacagga tttgtcttct ggttcaccgc
      541 tctggggctt ccattttgca cgtgggggct ta
//
LOCUS       DAZKJR010000490          569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZKJR010000490 DAZKJR010000000
VERSION     DAZKJR010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.493"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            356..>569
                     /locus_tag="ACHOWS_005529"
     CDS             356..>569
                     /locus_tag="ACHOWS_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFQ0050918.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESE"
BASE COUNT          190 a           95 c          123 g          161 t
ORIGIN      
        1 taatgatttt atataaatgt gaaaagcacc tcatttcata aataagcatg ttgtccggtt
       61 tctgatgaat ttgctaaaaa aggcaggcat ggtgaatccc cctgtgcggt ggggtgatac
      121 cattaatttc ctatgttgct gactggtatc acgaacagtc tgttcaccgg gaggcacccg
      181 gcaccatgct acataacact ccattaacgc tacactcgcc cctcttcgga ggggttttct
      241 ctgcaggata tgaatatgag atgcggcatt attcactgcg aatgatggtg agaatcttta
      301 ataaatttaa agtttatatg tattgttaca gtgttgatga aaggagataa aaacaatgtt
      361 cccggagtac agagaactga tttcagcatt gaaaggaaat cataaacgct ttgattcttt
      421 atttgaaaag cataataaac ttgatcacga aataaaaaga attgaagatg aatttggttc
      481 agcgtattcg acgaaagtgg tcaggctgaa gcttgaaaaa cttgagatca aaagagaaat
      541 ccagaaaatc cttgaagatg agtcagaaa
//
LOCUS       DAZKJR010000491          549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZKJR010000491 DAZKJR010000000
VERSION     DAZKJR010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.494"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            18..392
                     /locus_tag="ACHOWS_005530"
     CDS             18..392
                     /locus_tag="ACHOWS_005530"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFQ0050919.1"
                     /translation="MSEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLMVLLLNN
                     DVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNAEPGVLE
                     QTARAYIESHGTIERHLGCSDF"
BASE COUNT          135 a          115 c          143 g          156 t
ORIGIN      
        1 gtgaactgca tagactcatt tctgaatatt tgcagtgtaa gcgacgtaaa gatcaatctg
       61 ttgagcccac ccctttatat tatctctgga gtagggaagc tgtggctaac gataccactc
      121 cttatcttat ggtgctgcta cttaataacg atgtctggtg taagagtcgt gaggtggtgg
      181 ttctcagaaa tctgattgag caggcgtggg gaaaagtatt gggtgaagat aactccatta
      241 cacatctggt tgtgtatcct gaagtgctgt cgttggtatt tgatgcagct aacacataca
      301 acgctgagcc tggtgtactg gaacagacgg cccgggctta cattgaatca catggtacca
      361 ttgaacgtca tcttgggtgt agtgactttt aacgtcaggt gacgcattgt cacttctgac
      421 ctgctacctg tacgccggaa tataatggtc agagaggcat atttcgacgc ccctaatctg
      481 gggcgtttga ttcaggatgt ctggaacaat ccgggctttg tgtcaccggg cgcatcaaag
      541 cagtaccac
//
LOCUS       DAZKJR010000492          546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZKJR010000492 DAZKJR010000000
VERSION     DAZKJR010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.495"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(106..303)
                     /locus_tag="ACHOWS_005531"
     CDS             complement(106..303)
                     /locus_tag="ACHOWS_005531"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFQ0050920.1"
                     /translation="MPQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
BASE COUNT          155 a          131 c          136 g          124 t
ORIGIN      
        1 agcccgatac agagttgcaa ggacacactt ttgccagagg gcgttacagg aaagcttaac
       61 gtttgattct gtatacataa taaatcacct tacagttaca acaggtcaaa aaccgctgta
      121 gccagagtta cgctggcctg atgctttagt accgggcttc gtcagataat ccagacgctc
      181 caataagcgc tgatactgct cagggaaatc aggatcatga atatccagga tgtaacgtcc
      241 attagcaggg aaatgaataa tgcagccccc tggattaaca atgcagaaat cgtcctgagg
      301 cactgatcaa tacggagagg actctcgcgt gtggtttatt gacaccacag tgcagattcg
      361 gcgaatccgc gatcacggtg caatttcgtt ccacagcaca caatcataac cccgggtttt
      421 attcaggtaa gcaggactgc ggatatccgg tgtcgcgcct ttgtgtcacg aacggggtag
      481 gtgcgaaaca ccggataaaa tgcaggctgg caatacctct gaacgccctg cgcagagcgg
      541 atattt
//
LOCUS       DAZKJR010000493          546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZKJR010000493 DAZKJR010000000
VERSION     DAZKJR010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.496"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..119
                     /locus_tag="ACHOWS_005532"
     CDS             <1..119
                     /locus_tag="ACHOWS_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFQ0050921.1"
                     /translation="INILTNDNVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            129..546
                     /locus_tag="ACHOWS_005533"
                     /pseudo
     CDS             129..546
                     /locus_tag="ACHOWS_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          164 a          100 c          135 g          147 t
ORIGIN      
        1 ctattaatat tttaacgaat gataatgtat tcaaggcagg gttaagacgt aagatgcgaa
       61 aagcagccat ggacagaaac tacctggcgt cagtccttgc tgggagcggg ctttcgtaat
      121 ctttccctgg gcggtatatc atccaggact cgattgggct gaagggggga tggaatctgt
      181 accaatatcc gctgaatcca gtgcaacacg ttgatccatt agggcttagt actatgataa
      241 taggtaacgg ccccgttcct gataatcctt ttgggcatgc tgcggcggca aatcgatatg
      301 gattaatgag ttcgggaact ggggatgaga tgggagcctc tgtctctgat tattttaaaa
      361 agatgcaacc ccgcagagat acctggattt ggataataga tacaacagat gaagaagaac
      421 aatgtatgat gaataaggca atagacctta ataaaaaact aaaaactatt aacctcgggc
      481 ctcttcctgt tttaaataat tgttttagcc gaacgaacat gatttttgag gcgtgcgggt
      541 ttaaca
//
LOCUS       DAZKJR010000494          543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZKJR010000494 DAZKJR010000000
VERSION     DAZKJR010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.497"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..539)
                     /locus_tag="ACHOWS_005534"
     CDS             complement(<1..539)
                     /locus_tag="ACHOWS_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFQ0050922.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGHWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMP"
BASE COUNT          127 a          177 c          125 g          114 t
ORIGIN      
        1 ggcatcttcc tgaaccggaa ccggtatacg ccctccggta cagatatccc cggctgccag
       61 catctgccgt cggcctgttc gccttccagt tccgccagca acggcaggtg ataaaacaga
      121 gccgaccaga acaccaccat gttccacaca ctcccctgcg cggcctgctc ttctgctgtc
      181 gctcccggtg gaaacattct gcctctggcc agccggacag catacgtcgt gaactgcagg
      241 gtcaggtcac caaaaccacc ggtgtaaccc cagtgacctt ctgctgtggc cggcagattc
      301 tgactgacag acaacaactg atgcacagga tcaagacaaa ggcgctgata catatccacc
      361 ggcatggaac tgttttccca taactgtttc agacaccttt tcctgaacgg tgtggatacc
      421 agtacatcag ccggcaatgg tgtaaaatac ccctccggca ccgtttcccc gggaatatcc
      481 gtccggacat cagagcattc tgtcttcaca ggaaataacc ttttcaggcg ttcaagcatg
      541 ccc
//
LOCUS       DAZKJR010000495          541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZKJR010000495 DAZKJR010000000
VERSION     DAZKJR010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.498"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>541
                     /locus_tag="ACHOWS_005535"
     CDS             <1..>541
                     /locus_tag="ACHOWS_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFQ0050923.1"
                     /translation="SGASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQG
                     ESDFDGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPWICGDTTYFWKQKNES
                     TYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPDIGYYGSKWRTDRST
                     WTSQDRASHFSSWARRGIIS"
BASE COUNT          145 a          119 c          164 g          113 t
ORIGIN      
        1 cgagtggtgc ctcggagaat tcaacccgct ggggtgtgga caagccgctg tataaggacc
       61 ttatcggtcg tacaaaagcg gcactgaaga agaacccgaa aaatgttctg tttgccgtgg
      121 tgtggatgca gggggaatct gattttgacg gcacgccggc aaatcacgca gcacagtttg
      181 gtgcgctggt tgataaattc cgtgcagacc tggcggatat ggcaggccag tgcgtcggtg
      241 gctctgctgg cggtgttccc tggatatgtg gagacacgac gtatttctgg aagcagaaga
      301 acgaatccac gtaccagacg gtgtacggca gctataaaaa caaaacggaa aagaatatcc
      361 atttcgtgcc gttcatgacc gatgagaacg gggtgaatgt gccgacgaac aaaccggaag
      421 aagacccgga tattccggat atcgggtatt acggttcaaa atggcgaacg gacagaagca
      481 cctggacatc tcaggacagg gccagtcatt tcagttcatg ggcacgacgt ggaattattt
      541 c
//
LOCUS       DAZKJR010000496          539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZKJR010000496 DAZKJR010000000
VERSION     DAZKJR010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.499"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>539
                     /locus_tag="ACHOWS_005536"
     CDS             <1..>539
                     /locus_tag="ACHOWS_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050924.1"
                     /translation="AQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVR
                     GKKIEDFGEEIKGAAKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGAD
                     ADTLAMIALYRSDIPAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMP
                     SRYADTWQLLRTLPPSQMD"
BASE COUNT          166 a          136 c          155 g           82 t
ORIGIN      
        1 gcacagcagg cagaagtacg ccgacaggcc ggaggaaatc gtgacatccc gcagcctgag
       61 acaatcgcac ctgaatctga aacaactgtc tctactgaca gggaagctac cgtgcgcgga
      121 ggtgaagtca ggggcaaaaa aattgaagac tttggcgagg aaattaaagg ggcagccaaa
      181 caccgttatg cacagcttgc tgaaacactg ggtaaaacgc tggaagacag ggattatgcc
      241 acgcagccgc tgagcaaact gttcccgaaa ccggactacg caaaactggc gaacgaaggt
      301 gccgatgctg ataccctggc aatgatagcg ctgtatcgta gcgatattcc ggcgaagacg
      361 aaacacaata cggcaggctg gggggagagc ataaaaaaag tacgacacag tgtatcggaa
      421 atgctgaacg gaacggtcag cgcgaaacgc ctcgcagaat ggatggaagg cagaatgccc
      481 tcccgttacg cggacacctg gcaactgtta cgcactctgc caccctcaca gatggacag
//
LOCUS       DAZKJR010000497          536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZKJR010000497 DAZKJR010000000
VERSION     DAZKJR010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.500"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(219..410)
                     /gene="yfgG"
                     /locus_tag="ACHOWS_005537"
     CDS             complement(219..410)
                     /gene="yfgG"
                     /locus_tag="ACHOWS_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFQ0050925.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            428..>536
                     /locus_tag="ACHOWS_005538"
     CDS             428..>536
                     /locus_tag="ACHOWS_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050926.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVME"
BASE COUNT          174 a           93 c          126 g          143 t
ORIGIN      
        1 ttattaaaat tcatgcggga aataatttat aaaaatacat catcacaaca tatgtattaa
       61 tgatgtattt atttcgcagt cgaacaggat aaacatcagt caggacaaaa acgtaaaatt
      121 taagatgaaa aaatatctgc cgctttgata atcccaaaga gcaaaagcat ccgacagcga
      181 ggtgccgtta tgttctgtga gagtggagaa ggtaaccgct aacgttgtac tggtgattcg
      241 acggagagtg tggattgctg agcttctttt ttgctctgat ggtgctgcca ggcaccgacg
      301 gacgagtaga taaaacggcc aaagaagaag ataaagctga tgagcagtac gatacgggtc
      361 atgcgactgt taaatcggtg tcgttttcgc atactggttg cctgactcac aaaaggttcc
      421 ttgaagtatg tcccacgccg tggacggtac ttacattaag gcacaacagg acaaaatggt
      481 caattcttcg ttatgtaaaa aagcgtcagt ggatacatat tttaatgtta tggaag
//
LOCUS       DAZKJR010000498          529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZKJR010000498 DAZKJR010000000
VERSION     DAZKJR010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.501"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>529)
                     /locus_tag="ACHOWS_005539"
     CDS             complement(<1..>529)
                     /locus_tag="ACHOWS_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFQ0050927.1"
                     /translation="GYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLA
                     TNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAGGEFSGE
                     ITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRL
                     SAVWLTHDPEYPDNLP"
BASE COUNT           88 a          193 c          162 g           86 t
ORIGIN      
        1 ggcaggttgt ccggatattc cgggtcgtgc gtcagccaca cggcagacag acggatgccg
       61 ttgtcccggc catattcggt gtaacccggc agggtatcag gaaaagcgga cggttccgtc
      121 ccgccggaaa tggcctgctg ccgggcctct tctgcccgct gcgcctgcgt ggtcagtacc
      181 agccggaagt gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcgccg
      241 ccagcctcac ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc
      301 cggtacggcg gcagcggcgc aggcagcacc tcatccgcct ccggcacccg ctcacaccag
      361 ccgagtaccc accacggccc ctgcggactg ttcgtcgcca gataacgatg cggactcagg
      421 cgaagctctt ccggcagcgc ctgccagagt gcggccagcc gatgaccttc atccagtttc
      481 gccacgccgc cgcgcaccag ccagagtgac tcgctgcggc tgtaaccgt
//
LOCUS       DAZKJR010000499          527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZKJR010000499 DAZKJR010000000
VERSION     DAZKJR010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.502"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..>527
                     /locus_tag="ACHOWS_005540"
     CDS             87..>527
                     /locus_tag="ACHOWS_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HFQ0050928.1"
                     /translation="MDFSTLITFSIITLSQTVSIGPGVALIISESLTYGAKKAITTSI
                     YIRFGEIVVMLVTLFSIALAINLEKHLDTIKTCGGGYLIFLGITRLIKRKGSKGNKAN
                     IPFLIPMLNPKAFLFFAAFMPSFIKNNSNFNTDFLLLCIIFIIVS"
BASE COUNT          174 a           92 c           83 g          178 t
ORIGIN      
        1 tataatgttg cttcctgagg cagccatacc tatggagtat gtttatttta aggttccaac
       61 cagaataaca ggagttatat aatcccatgg acttctcaac attaataaca ttctctatta
      121 taaccttatc acaaacagta tcgattggtc cgggggtggc attaatcata tctgaaagtc
      181 tgacatatgg tgcaaaaaaa gcaataacga caagtatata tataagattt ggcgaaattg
      241 ttgtcatgct agtcacacta ttttctatag cactggcaat caatttagaa aaacatcttg
      301 acacaataaa aacttgtggt ggtggatatc tgatttttct tggtattact cgtctcatca
      361 agagaaaagg atctaaagga aacaaagcaa atatcccttt tttaataccg atgctcaatc
      421 caaaggcctt tttgttcttt gctgcattta tgccatcatt cataaaaaac aacagtaact
      481 ttaatactga ctttttgtta ctttgtatca tttttataat tgtgtca
//
LOCUS       DAZKJR010000500          524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZKJR010000500 DAZKJR010000000
VERSION     DAZKJR010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.503"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            109..230
                     /locus_tag="ACHOWS_005541"
                     /pseudo
     CDS             109..230
                     /locus_tag="ACHOWS_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            258..524
                     /locus_tag="ACHOWS_005542"
                     /pseudo
     CDS             258..524
                     /locus_tag="ACHOWS_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
BASE COUNT          125 a          115 c          150 g          134 t
ORIGIN      
        1 caggtctttg aatttgttag ccagatttct gttctaattt tggatagcag aatatgcaat
       61 gttatttttc gaatcctcca taactgatat atgtgctgtt agtggagtac aagaataagc
      121 tcgcgttagc agtctgtcag tactgcgaca gcgttgtctg ggagggcttt acccggtcaa
      181 aacagcatta ttgtgcaccc catgtgacgg atgaactgtg tgctcagggg gcattttact
      241 gtaaaaaccg tgctgcggtc agccgtaacc gtcaggtggg tgaacgcaac agtcgcgcca
      301 ttgcttttca ggcacgtacc ctgcaggcgc atgagcgtcg tatgaacagc cagcagcgtc
      361 agattaatga gaatcatgag gaaatgaagc gggcagcagc ccagtcagct gcgctggccg
      421 gtcttttcca gccatacagt gtgggtaaat ttaacgccac agcggctgtg ggcggacagt
      481 gacaggcagg ctgttgcggt gggcatggga tatcgcttta acga
//
LOCUS       DAZKJR010000501          524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZKJR010000501 DAZKJR010000000
VERSION     DAZKJR010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.504"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(24..125)
                     /locus_tag="ACHOWS_005543"
                     /pseudo
     CDS             complement(24..125)
                     /locus_tag="ACHOWS_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(381..>524)
                     /gene="tdcR"
                     /locus_tag="ACHOWS_005544"
     CDS             complement(381..>524)
                     /gene="tdcR"
                     /locus_tag="ACHOWS_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFQ0050929.1"
                     /translation="RYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECN
                     RFP"
BASE COUNT          158 a          103 c          102 g          161 t
ORIGIN      
        1 catttggacg ttataaccta aatttttacc gtccaataat caatatttct gttgtgattg
       61 ttaaccactg cgtgcattgg cgggattgaa gggtgaaaaa tatgtgcaat tgggaagcct
      121 ttcattgatg cctgccttgt catcatgaca aacaataata gatgcttatt ttcctcgcag
      181 ggttctttcg cactatcaaa aaatgctcat ttacccggca ttacagtcaa cgaagcaaca
      241 ttctatggcc tacgctgatg taaagcacag tgagaatccc ccttgttgtc ttcgtctgac
      301 cttatcgcgg cgaccagcaa ttattcgttt tgtgatcctc aacacaaaat gctattcgtc
      361 gcctatgagg ggagaggaat ttatggaaaa cgattgcatt cacaaaatga cgtcctgtca
      421 gaagagaaaa agttgacgtt aatcaattgt tcgagatgat cgatacctgt attcgaaatt
      481 ttaaaatatt gataaaataa atgtttgttc tccaacaaat agcg
//
LOCUS       DAZKJR010000502          522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZKJR010000502 DAZKJR010000000
VERSION     DAZKJR010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.505"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..499
                     /locus_tag="ACHOWS_005545"
     rRNA            <1..499
                     /locus_tag="ACHOWS_005545"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          130 a          127 c          153 g          112 t
ORIGIN      
        1 acaggtgctg catggctgtc gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac
       61 gagcgcaacc cttatccttt gttgccagcg gtccggccgg gaactcaaag gagactgcca
      121 gtgataaact ggaggaaggt ggggatgacg tcaagtcatc atggccctta cgaccagggc
      181 tacacacgtg ctacaatggc gcatacaaag agaagcgacc tcgcgagagc aagcggacct
      241 cataaagtgc gtcgtagtcc ggattggagt ctgcaactcg actccatgaa gtcggaatcg
      301 ctagtaatcg tggatcagaa tgccacggtg aatacgttcc cgggccttgt acacaccgcc
      361 cgtcacacca tgggagtggg ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct
      421 taccactttg tgattcatga ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg
      481 cggttggatc acctccttac cttaaagaag cgtactttgc ag
//
LOCUS       DAZKJR010000503          521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZKJR010000503 DAZKJR010000000
VERSION     DAZKJR010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.506"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>521
                     /locus_tag="ACHOWS_005546"
     CDS             <1..>521
                     /locus_tag="ACHOWS_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFQ0050930.1"
                     /translation="RITQKLKEGWQPYGSALISTAGYGAEFIQPVVSEGSISSPEEPG
                     NRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTFDSPDPRIKQLARRSTVTPG
                     GAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGCVGQGLHIAKKLLPFIPANA
                     GILLVPCCRGGSA"
BASE COUNT          121 a          123 c          147 g          130 t
ORIGIN      
        1 cgaataactc aaaaactgaa ggaagggtgg cagccttatg gtagtgcgct gatttcgaca
       61 gctggttatg gtgcggagtt catccagcca gttgtgagtg aggggagcat ctcatcacca
      121 gaggagccag gcaaccgtcc gacgacctca gcgccttctg ttgcgccaga atattactat
      181 gtgatcgcgc ttgctggtca gtccaatggt atgtcatacg gtgagggact gccattaccg
      241 gatacattcg acagccctga tccacgtatt aaacagttag cgcgtcgcag tacggtgaca
      301 ccgggaggtg ctgcatgtat ctataacgat attatcccgg cagatcactg tctccatgat
      361 gtggaggata tgacagggct taatcatccc aaagcggatt tatcgaaagg tcagtacggc
      421 tgtgtgggac aggggctgca tatcgccaaa aagcttctgc catttatacc ggcgaatgcg
      481 ggcattcttc ttgttccgtg ctgccgtggt ggttcagctt t
//
LOCUS       DAZKJR010000504          518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZKJR010000504 DAZKJR010000000
VERSION     DAZKJR010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.507"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..>518)
                     /locus_tag="ACHOWS_005547"
     CDS             complement(107..>518)
                     /locus_tag="ACHOWS_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050931.1"
                     /translation="QISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAYLSRC
                     LASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDTVIFS
                     ILSSLIDSTISKFQHQNPVNGSVNPDLLYEDYTD"
BASE COUNT          160 a           77 c          119 g          162 t
ORIGIN      
        1 cgtaaaaaag aaaagtacat gaaggtgcta ccagtacgag aaacgttaat caaaagtttt
       61 attacgtctg gcaataacgc aggccatttt tcgcgacagg agggatttaa tcagtataat
      121 cctcatacaa taggtcagga tttacagaac cgttaacggg gttttggtgt tgaaatttag
      181 aaatggtgga gtcaatcaat gaagacagta tcgaaaaaat gactgtgtca gaattaattt
      241 caattaatcc ttctcgttgt tgaccaaaaa agatatttat cttgatttca tttgtactac
      301 ggcaatcaat atgtagtaat ttttgatcgc tgagattttc tgtttcataa atgtttttgg
      361 cgatagtcgg atgtattgat gccagacatc tgctcagata agccagatcc tttagggaac
      421 accccgctga gtagaggatg tcagagaatt tttgtgtgtc aactgttggc tctgaagcgt
      481 atgttgcatt gactggaagg gtgttgaagg agatttga
//
LOCUS       DAZKJR010000505          517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZKJR010000505 DAZKJR010000000
VERSION     DAZKJR010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.508"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>517
                     /locus_tag="ACHOWS_005548"
     CDS             <1..>517
                     /locus_tag="ACHOWS_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050932.1"
                     /translation="DVLLWKLGKNDSRRRSGVYYLNAYKDAYVQYNRDKIIKHAYAAG
                     IRPELLGGVAWIESGGMPENYKFQIYETKRMIGSLDMPENKTSFGSMGIQIRTAAITL
                     GLDPSELTTRNQLELATCLMEDDFTFKIAATHLRDLALFDYPSSATLYMTNEQYIMAG
                     IRYNRGVERDLG"
BASE COUNT          165 a           84 c          115 g          153 t
ORIGIN      
        1 ggacgtttta ctctggaagt taggaaaaaa tgattcgcgt cgccgttcag gagtatatta
       61 tttaaatgct tataaagatg catatgtaca atataatagg gacaaaataa ttaaacatgc
      121 atatgccgca gggattcgtc cagagttact gggcggcgtg gcctggattg agtcaggagg
      181 tatgcctgaa aattataagt ttcagatata tgaaacaaaa agaatgatcg gatcgttaga
      241 tatgcctgaa aataaaacat catttggctc aatgggtata caaatacgaa ccgcagcgat
      301 tactctaggc cttgaccctt cagaattaac taccagaaat cagttagagt tagcgacatg
      361 cctgatggaa gatgatttta cctttaaaat tgctgctact catttaagag atcttgccct
      421 ttttgattat ccctcttcgg caacactgta tatgacgaat gagcagtata ttatggctgg
      481 tattagatat aatcgtggtg ttgaacgaga tttgggt
//
LOCUS       DAZKJR010000506          511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZKJR010000506 DAZKJR010000000
VERSION     DAZKJR010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.509"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>511)
                     /locus_tag="ACHOWS_005549"
     CDS             complement(<1..>511)
                     /locus_tag="ACHOWS_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050933.1"
                     /translation="AESRNVEREALIDSEILRGQRCAWILGTYIQSPAGNKADDLLEA
                     MEVAAPAIDFSHPRGCDKPVRYAALPEYQTDLTKALKGAVNKLTTRVEGIVHPLPPAL
                     PCWLVLDCDNDLYPLIEEQLKADLSLKTGRIFRLMTGKGLGAFDAWLDKRWDTPGILV
                     VITLSLPASP"
BASE COUNT          115 a          150 c          131 g          115 t
ORIGIN      
        1 gggagaggca ggtaacgaca gcgtgatgac aaccagaatg cccggggtgt cccatcgctt
       61 gtccagccag gcatcaaacg cccccagccc ttttccggtc atcaggcgaa aaatcctgcc
      121 cgtcttgagt gagaggtcgg ctttaagctg ctcttcaatc aggggataca gatcgttatc
      181 acaatccagc accagccagc atggtagcgc gggcggaagc ggatgaacta tcccctctac
      241 acgcgtggtc agtttattca ccgccccttt cagagccttc gttaaatctg tctggtattc
      301 tggtaatgcc gcgtacctga ccggtttatc acatcctctt gggtgcgaga aatcaatagc
      361 aggagccgcg acttccattg cctcaagcag gtcatcggct ttgtttccgg ccgggctctg
      421 aatataagta ccgaggatcc aggcacagcg ctgaccacgc agaatttcac tgtcaataag
      481 cgcttcccgc tcgacatttc gggactcagc c
//
LOCUS       DAZKJR010000507          508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZKJR010000507 DAZKJR010000000
VERSION     DAZKJR010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.510"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>508
                     /locus_tag="ACHOWS_005550"
     CDS             <1..>508
                     /locus_tag="ACHOWS_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFQ0050934.1"
                     /translation="EDLIESPEISGVDSGDKNIEEQSESELAPEQKRTGIAKMLMTTP
                     VLTLIFDRVRYDEIELQPDFQRKDRIWPDDKKSKLIESILMKLPLPGFYFGEKPNGNW
                     VVIDGLQRTTTICDYMSGHFSLKGLSILEHLNGKSFKDLTRTEQRDIREYQITAYQIE
                     LNDDSSELV"
BASE COUNT          177 a           77 c          108 g          146 t
ORIGIN      
        1 gaggatttaa ttgagtcccc tgagataagt ggagttgatt caggtgataa gaatattgag
       61 gaacaatcgg aatcagaatt agcacctgag caaaaacgta cgggcattgc aaaaatgctt
      121 atgactacac cagttttaac actaattttt gatcgtgtaa gatatgatga aattgagtta
      181 caacctgact ttcagagaaa agaccgaatt tggcctgacg acaaaaagtc aaaacttatt
      241 gaatcaatat taatgaagct tccattaccg gggttttact ttggtgaaaa gccaaacggt
      301 aactgggtag ttattgatgg ccttcaaaga acgactacaa tttgtgatta tatgagtggt
      361 catttctcat tgaaagggct ctctatttta gagcacttaa atggaaagag ttttaaagat
      421 cttactcgaa ccgagcaacg agacattaga gagtatcaaa ttacggctta ccaaatagaa
      481 ctaaatgatg atagttctga attggttg
//
LOCUS       DAZKJR010000508          493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZKJR010000508 DAZKJR010000000
VERSION     DAZKJR010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.511"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>493
                     /locus_tag="ACHOWS_005551"
     CDS             <1..>493
                     /locus_tag="ACHOWS_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFQ0050935.1"
                     /translation="KGGWNFYQYPLNPITNIDPLGLETLKCIKPLHSMGGTGERSGPD
                     IWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVET
                     DNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNI
                     GRFI"
BASE COUNT          158 a           77 c          124 g          134 t
ORIGIN      
        1 aaggggggat ggaattttta tcagtatccg ttgaatccga tcactaatat agatccttta
       61 ggattagaaa cactaaaatg cattaagcca ctgcattcaa tgggcggaac tggtgaaaga
      121 agcggtccag atatatgggg gaatccgttc tatcatcaat atctttgtgt cccagatggt
      181 aaaggggact atacttgtgg tggccaagac caacggggag aatcaaaagg agatggtcta
      241 tgggggccag gtaaagcaag taatgataca aaagaagctg ctggccgttg tgacctcgtt
      301 gaaaccgata atagttgtgt ggagaactgt ttaaaaggga agtttaaaga ggtaaggccg
      361 cgttattctg tattgcctga tatattcaca cctataaatt tagggctatt taaaaactgc
      421 caagactggt ctaatgattc tttagaaaca tgtaagatga agtgctccgg aaataacatt
      481 ggacgtttta tta
//
LOCUS       DAZKJR010000509          488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZKJR010000509 DAZKJR010000000
VERSION     DAZKJR010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.512"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..352
                     /locus_tag="ACHOWS_005552"
     CDS             <1..352
                     /locus_tag="ACHOWS_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFQ0050936.1"
                     /translation="SLTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRL
                     NAGIAGVTAISSIPYVAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDS
                     SHNTAIGAGVAGGW"
BASE COUNT          148 a          125 c          112 g          103 t
ORIGIN      
        1 ttcactgaca ctgaaacagg caacagccta cagcgatatc agtacccgcc gggcactgtc
       61 agaggccaat cgttataccg acaacaaatt cagaaacctg agcaataaaa ttgaacgggc
      121 agaaaaaaga cttaatgcag gcatcgccgg agtgacagca atatcctcca tcccctatgt
      181 ggctgaaaat tctttttcat acgggatcgg cgtgggtaac tatcagaacg ctaatgctgt
      241 ggccgcaggc attcagtata aaacatcacg taataccaat atacgcctca acatctcctg
      301 ggactcatct cataacaccg cgattggtgc aggggttgcc ggcggctggt aaataatctt
      361 gcgtacatac ggcattatgg tattccgtaa tcacattaat tcagtacgca acacagcccc
      421 atccggaaca gaccactgga ctgagcggga taatcaagga aaaagggcgc gcagtgtggt
      481 cagccttc
//
LOCUS       DAZKJR010000510          485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZKJR010000510 DAZKJR010000000
VERSION     DAZKJR010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.513"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     repeat_region   67..400
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=67..95
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          118 a          121 c          129 g          117 t
ORIGIN      
        1 tactgtaaag ttggtagatt gtgactggct taaaaaatca ttaattaata ataggttatg
       61 tttagagagt tccccgcgcc agcggggata aaccgagatt tcagaggcag ccgccaggcc
      121 gttgatcgag ttccccgcgc cagcggggat aaaccgccat ttcggtagcg ccatcaaaat
      181 ggttacgcga gttccccgcg ccagcgggga taaaccgcgt cgttagcggt ctggtgacgt
      241 taaaggggtg agttccccgc gccagcgggg ataaaccgtt gatcgccgtg gcggtgagat
      301 tagaaacgcg gagttccccg cgccagcggg gataaaccgg aagtggcaaa gctgccgttt
      361 cttttctcta tgagttcccc gcgcctgcgg ggataaacca ccatataacc cgttatctct
      421 ttctcaagtt tttatattag ctgtacttgt aataagcaac atatccacgt aacacctcat
      481 gttca
//
LOCUS       DAZKJR010000511          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZKJR010000511 DAZKJR010000000
VERSION     DAZKJR010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.514"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..438
                     /gene="tssJ"
                     /locus_tag="ACHOWS_005553"
     CDS             <1..438
                     /gene="tssJ"
                     /locus_tag="ACHOWS_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFQ0050937.1"
                     /translation="GMISITKSIFYKKIKTLHLDFTPRTAINTDGAQTPLATMVRVYQ
                     LRDRKAVDAADYQMLLRRADTVLKDDVLASKELLVMPDGSVTLNMPMDEDAQFVAVVG
                     LFNRPDQKDNRWRLVLTRDDLNPDKPRTIELGDGWLSLVPVKE"
BASE COUNT          114 a          124 c          142 g          103 t
ORIGIN      
        1 ggtatgatca gcatcacgaa atccatcttc tacaaaaaaa tcaaaacgct gcatctggat
       61 tttaccccgc gtacggccat taacactgat ggggcacaga cgccactggc aaccatggtg
      121 cgggtttacc agctcaggga tcgtaaagcg gttgatgccg ccgattacca gatgctgctg
      181 cgccgggccg atacggtact taaggacgac gtgctggcct caaaagaact gctggtgatg
      241 cccgacggca gcgtgacgct caatatgccc atggatgaag atgcgcagtt tgtggctgtg
      301 gtgggactgt ttaaccggcc ggatcagaag gacaatcgct ggcgcctggt actgacccgt
      361 gatgacctca atccggataa gccccgcacc attgaactgg gtgatggctg gctcagtctt
      421 gtgccggtaa aggagtgacg tgatggagaa ccggtcagcg ttttttgacg gacagccccg
      481 tta
//
LOCUS       DAZKJR010000512          482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZKJR010000512 DAZKJR010000000
VERSION     DAZKJR010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.515"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..250
                     /locus_tag="ACHOWS_005554"
     CDS             <1..250
                     /locus_tag="ACHOWS_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050938.1"
                     /translation="IKGVRHSIALAQKLVGDTVKLGNEGINVLTMLTDLADVVEELAD
                     ITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            complement(287..>482)
                     /locus_tag="ACHOWS_005555"
     CDS             complement(287..>482)
                     /locus_tag="ACHOWS_005555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050939.1"
                     /translation="GAQKSFSDVRGSNRAGGIISSLRGVTRRFTWGLRGAFRPSFFLL
                     RTVFSWRVMSGDEYFLLSGE"
BASE COUNT          144 a          138 c          110 g           90 t
ORIGIN      
        1 gattaagggc gtccgtcaca gtattgcgct ggcgcagaag ctggttggcg atacggtgaa
       61 gctgggaaac gagggcatta atgtactgac catgctgacg gaccttgccg atgtggtgga
      121 agagctggca gatattacgg catcacatac ccaccccaaa acgggcacgt ctccgcaggc
      181 cgcacagttc agccaggtcg ctcaggagtg ccgccagctt aaaaataaat attcaccaat
      241 aatcgagtaa ttattcactt taacaggccg cgcaatgtgg ccttttttat tcccccgaca
      301 ataaaaaata ttcatcaccg gacatcaccc gccacgaaaa aaccgtccgc agcagaaaaa
      361 aagatggacg aaacgcccca cgtaatcccc acgtaaaacg tcgcgtaacg ccacgtaacg
      421 aagaaataat cccgccagcc cgattcgatc ccctgacatc agaaaacgat ttctgcgccc
      481 ca
//
LOCUS       DAZKJR010000513          481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZKJR010000513 DAZKJR010000000
VERSION     DAZKJR010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.516"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>481
                     /locus_tag="ACHOWS_005556"
     CDS             <1..>481
                     /locus_tag="ACHOWS_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0050940.1"
                     /translation="DSGRITTFLKTHLGGLVHQEGNHWVAEPSASQGMVVSPDFLRAI
                     NQLAELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNLDYFNQMESWQSFTWPG
                     NTYYPGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPLDASRTQLVWITADGNP
                     "
BASE COUNT           99 a          126 c          146 g          110 t
ORIGIN      
        1 gcgactcggg tcggatcacg acgttcctga aaactcatct tggcgggctc gtccaccagg
       61 aggggaatca ctgggtcgct gagccttccg caagccaggg gatggttgtc agtccggatt
      121 tcctccgggc gattaatcag ctggcagagc tgtcagatat cgtgttctct cagggcgatg
      181 cgggggttca cttcgaactg atggcgcgtc cttcacggga tgttgccaga gtgcagctta
      241 cccttgatga acagaatctg gattacttca atcagatgga aagctggcag agcttcacct
      301 ggccggggaa tacttactat ccaggtgtgt cgctcagctg gcggagtgta agcagtggta
      361 tgcaactcta cgccagtcac caggggaact gggggtttat tcgcctgctg gacgaagccc
      421 tgatcacgcc acttgatgcc agccgcacac aactggtgtg gatcactgcc gatggtaatc
      481 c
//
LOCUS       DAZKJR010000514          480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZKJR010000514 DAZKJR010000000
VERSION     DAZKJR010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.517"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(227..>480)
                     /locus_tag="ACHOWS_005557"
     CDS             complement(227..>480)
                     /locus_tag="ACHOWS_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050941.1"
                     /translation="IFEHNGRCFMKKNETWMLLNGDATPNIRTMSNKKIECLEKSEVE
                     WASRVCDSITDKERENLKALICSKPAKSVNTGKRPALGY"
BASE COUNT          114 a          105 c           86 g          175 t
ORIGIN      
        1 cagcttcagg taatgaggaa tatctcaatt tttaaacata aaatgcgaat tatttagtac
       61 aaaaaagcag cgttgcgtga tctttcccta gtccctgtcc cggattgtag taccggtgcc
      121 agttgtagtg cagttcggat tcgtcgtcat actgctagcc cggatggcgg agcagttgct
      181 ctagtcactc agaatattgt aatagtgata gagtgcagtg ccggtttcaa tatcctaacg
      241 ctggtctttt gcctgtatta actgatttag ctggtttgct acatattaat gccttcagat
      301 tttccctttc cttgtctgtg atactgtcgc aaaccctact agcccattct acttcactct
      361 tctccaggca ttctattttc ttattgctca ttgttctaat gtttggtgtt gcatctccat
      421 ttaataacat ccatgtttca tttttcttca taaaacatct tccattatgt tcaaagatat
//
LOCUS       DAZKJR010000515          474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZKJR010000515 DAZKJR010000000
VERSION     DAZKJR010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.518"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..371)
                     /gene="subB"
                     /locus_tag="ACHOWS_005558"
     CDS             complement(<1..371)
                     /gene="subB"
                     /locus_tag="ACHOWS_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFQ0050942.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKVLTSNALVGLS"
BASE COUNT          151 a          117 c           82 g          124 t
ORIGIN      
        1 gataacccaa caagggcatt ggacgttaat accttcacaa agggcggata cgtccatacc
       61 ccgggttcat aataaatcct gaccaactga cctgttgtgt aaaaatatag tgcctgatta
      121 tataatgtgg aaaacgaagg cccccagaca gatgaattct tcatcgagca ggctcttatg
      181 gaagaaccac caggttgttt cccttcaata caaaaataag ggttattatc tatttgccct
      241 gtatgaaact gatcaataac aacgcctgaa aacatgccat cccgggcatc gccagtccac
      301 tcagccatgg caggattaag ggataaacat cccataacac ctgcacatac aaaaaacctc
      361 ttaatcgtca tattcacctc cggaatttta cagttcttca ctcatccttc cctgacggac
      421 aggaatataa tttttcttgc agaacatgct aattgctttt tctgcattaa gaac
//
LOCUS       DAZKJR010000516          473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZKJR010000516 DAZKJR010000000
VERSION     DAZKJR010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.519"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>473)
                     /locus_tag="ACHOWS_005559"
     CDS             complement(<1..>473)
                     /locus_tag="ACHOWS_005559"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0050943.1"
                     /translation="HIQNKPMLNYANSARLRKFIIIGTLCEGKGQKIAIEAMHNLIRE
                     GYLCHLKIIGNNRVPYASYLNKIVADYNLSDYVEFMGFRHDLDQIRLDNDVCLIPSLS
                     EAFGRVTIESMAAGMIVVASDSGASKEIINDGINGFLFSSGSVSDLTSVLKKILD"
BASE COUNT          164 a           99 c           64 g          146 t
ORIGIN      
        1 atcaaggatt tttttaagta cgctagtaag atcacttacc gaacctgaag aaaacaaaaa
       61 accatttata ccatcattga tgatctcttt actagcccca gaatcactag caacaactat
      121 catacctgca gccattgatt caatggtaac tctaccaaaa gcttcagaaa gagagggaat
      181 taagcaaaca tcgttgtcaa gccttatttg atcaagatca tgcctaaatc ccataaactc
      241 tacataatca ctcaaattat aatcagcaac aattttatta agataacttg catatggaac
      301 tctattattt ccaattattt ttaagtgaca taaatatcct tcacgaatca gattatgcat
      361 agcctctatg gctatttttt gacctttgcc ctcacacaat gtgccaataa taatgaactt
      421 ccgcagccta gccgaattag cataatttag cattggtttg ttttggatat gga
//
LOCUS       DAZKJR010000517          473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZKJR010000517 DAZKJR010000000
VERSION     DAZKJR010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.520"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHOWS_005560"
     CDS             complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHOWS_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFQ0050944.1"
                     /translation="TKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYFNTK
                     LENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNERTTA
                     "
BASE COUNT          112 a          128 c          108 g          125 t
ORIGIN      
        1 ttcactcact gtcagaagac cttcagacac gctaccgccc ttcgccataa cccgggcgca
       61 tcaggtctgt gttcaggatt gagaattctt tcttcagcac ccgccaacag atactgaaga
      121 aaaaagaaaa atgacggaca ggataccctg cccgtccggg agaattacgc agtggtacgt
      181 tcattccacg cgtcagaatg aatgatgtta ccgtccttat aagtccaggt gattttctcg
      241 tagcgcagct ccacctgctc aaggtggtta tgcttctcgt aggcgggatt cttgatgtcg
      301 tgcatgaccg ggttaacctt caccaccttc acgttctcaa gcttcgtatt gaaatactcc
      361 acttcctgac cggcatcatt gattctgtac cacttaaatt cagcagactt cagggtctgc
      421 cccgtggtca ccgccttgta aaggtagggg ctggaggaat caatttcctt ggt
//
LOCUS       DAZKJR010000518          471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZKJR010000518 DAZKJR010000000
VERSION     DAZKJR010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.521"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..222
                     /locus_tag="ACHOWS_005561"
     CDS             <1..222
                     /locus_tag="ACHOWS_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFQ0050945.1"
                     /translation="GVAKVTADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNE
                     SVTLDVAYEGSGSGDWRTSGVTAGIGLKF"
BASE COUNT          120 a          106 c          130 g          115 t
ORIGIN      
        1 ggcgtggcta aagttaccgc tgacctgaaa attaaggaca atattaacag tagtggcgga
       61 ttttctgaaa gcaacagcac gaaaaaaacc tcccttgcgt gggctgcagg tgcacagttt
      121 aacctgaatg agagtgttac actggatgtg gcttacgaag gttccggctc tggcgactgg
      181 cgcacgagtg gcgttactgc tggcattggc ctgaaattct gacctgtatc cggtaaccat
      241 ttactacccg ctgtgatggc gggtttttta ttgcccgtac agggcaaaaa tcgtaaatta
      301 tgcgtgggtg cctttcggct gatggctgga gggtgaacct gaaggcctga tgtggaaagg
      361 ccccgagtca acttaacgtt aacccgaggc cctaacactt cgtaccttaa gcaagtagaa
      421 ggttagcgcc tctctgtaaa aggagtcaag cgctatgtcg caaaaatcgc t
//
LOCUS       DAZKJR010000519          470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZKJR010000519 DAZKJR010000000
VERSION     DAZKJR010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.522"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(219..452)
                     /gene="ydfK"
                     /locus_tag="ACHOWS_005562"
     CDS             complement(219..452)
                     /gene="ydfK"
                     /locus_tag="ACHOWS_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFQ0050946.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNKNLNGKM"
BASE COUNT          132 a           75 c           92 g          171 t
ORIGIN      
        1 aaacgaatgc gtatgcaacc ccgtaatttt ggtgagaccc aaaatcgatt ttgtgaaaaa
       61 tggccttaac tcggtttgtt tttcgagttc cgggcggact caaggaagaa gaatagtgtt
      121 gcgtgttatt ttaaccagat ttcaagttgt ttggtcgtgg aaaagtggag caaaatgttg
      181 ttaaagtgga aaaatgataa aaaagtaagt ttattatatt acattttacc atttaaattt
      241 ttgttgtctt taagaactga tatcgctgtt tgtaataatt ctttgttatc cagccatgac
      301 tttttcttta tgtttccttc aatgtaatca agcaatgttc tggtattgat aggtcttccc
      361 tgttttgcta cttccactac agcatcccct aggataattc ttacttcagg aagctgcgca
      421 gggaaccact ttagggtgtc ttttgatttc atgaagatat tccttaaaat
//
LOCUS       DAZKJR010000520          470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZKJR010000520 DAZKJR010000000
VERSION     DAZKJR010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.523"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>470
                     /locus_tag="ACHOWS_005563"
     CDS             <1..>470
                     /locus_tag="ACHOWS_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0050947.1"
                     /translation="NRSFTSALKIKKEKVVVFNSISKTFNVPALTGSYLISTDEDYNH
                     KFRTISRYRDFVNSPSVLNIIATIVAYNECEGWLKSLKAHIASNIQFTQQYLNENTPE
                     LIVRPADGCYFSWIDCSAIGYSFDEFYSRLIHEGKVGIMAGNVYGTEGEGYLRL"
BASE COUNT          145 a           84 c          102 g          139 t
ORIGIN      
        1 taatcgtagc tttacatccg cgcttaaaat aaaaaaagaa aaagtagtgg tatttaactc
       61 gatatcgaaa acatttaatg ttcctgcgct aacaggatcg tatctgattt caactgacga
      121 ggattataat cataagttca gaacgatatc gaggtatcgc gatttcgtaa actccccatc
      181 agtactgaat ataattgcaa ccattgttgc ctataatgaa tgtgagggat ggttaaagtc
      241 attaaaggca catatcgcct ctaacattca attcacccag cagtatctga acgaaaatac
      301 tccagaattg atcgtaaggc cagctgatgg ttgctatttt tcgtggatag attgctctgc
      361 tattggttat tcctttgatg aattctacag tcgattaatt catgaaggca aagtgggcat
      421 tatggcgggg aatgtctatg gaacggaggg ggagggctac cttcgtctga
//
LOCUS       DAZKJR010000521          469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZKJR010000521 DAZKJR010000000
VERSION     DAZKJR010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.524"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..222
                     /locus_tag="ACHOWS_005564"
     CDS             <1..222
                     /locus_tag="ACHOWS_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFQ0050948.1"
                     /translation="GVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNE
                     SVTLDVAYEGSGSGDWRTSGVTAGIGLKF"
BASE COUNT          117 a          108 c          130 g          114 t
ORIGIN      
        1 ggcgttgcta aagttagcgc tgacctgaaa attaaggaca atattaacag tagtggcgga
       61 ttttctgaaa gcaacagcac gaaaaaaacc tcccttgcgt gggctgcagg tgcacagttt
      121 aacctgaatg agagtgttac actggatgtg gcttacgaag gttccggctc tggcgactgg
      181 cgcacgagtg gcgttactgc tggcattggc ctgaaattct gacctgtatc cggtaaccgt
      241 ttactacccg ctgtgatggc gggtttttta ttgcccgtac agggcaaaaa ccgtaaatta
      301 tgcgtgggtg cctttcggct gatggctgga gggagaacct gaaggcctga tgtggaaagg
      361 ccccgagtca actttaacgt taacccgagg ccctgaccgt acaaccttca caagtagtag
      421 gttagcgcct ctccattcag gagacaagcg ttatgtcgca aaaatcgct
//
LOCUS       DAZKJR010000522          466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZKJR010000522 DAZKJR010000000
VERSION     DAZKJR010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.525"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          127 a          116 c          119 g          104 t
ORIGIN      
        1 ataaatcacc ttacagttac aacaggtcaa aaaccgctgt agccagagtt acgctggcct
       61 gatgctttag taccgggcat cgtctgataa tccagacgct ccaataagcg ctgatactgc
      121 tcagggaaat caggatcatg aatatcctgg atgtcacgtc cattagcagg gaaataaaca
      181 atgcagcccc ctggattaac tatgcagaaa tcgtcctgag gcactgataa atacggagag
      241 gactctcgcg tatggttggt tgacaccaca gtgcggattc ggcgaatccg cgatcacggt
      301 gcgatttcgc accacagtac acaatcaata ccccgggttt tattcaggta agcaggactg
      361 cggatatccg gtgtcgcgcc tttgtgtcac gaacggggtg ggtgcgaaac accagataaa
      421 atgcaggttc cgatacctct gaacgccctg agcaggtcgg gcattt
//
LOCUS       DAZKJR010000523          466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZKJR010000523 DAZKJR010000000
VERSION     DAZKJR010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.526"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>466)
                     /locus_tag="ACHOWS_005565"
     CDS             complement(<1..>466)
                     /locus_tag="ACHOWS_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050949.1"
                     /translation="SIPSGKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNP
                     GVTTVYAFANMVTPPSEYGIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCA
                     IMPALVGYYNWKRTPYDRPIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDK"
BASE COUNT          132 a          107 c           90 g          137 t
ORIGIN      
        1 cttatctata tacggctttc gaatatccca tgttgcacca ccggaataat gctcacagga
       61 atatatttta tttcctgtag caccagtgga tgtggtataa atcggtcgat cataaggagt
      121 tcttttccag ttataatacc cgaccagtgc aggcatgatt gcgcatggat atccaaggtc
      181 cttttcaaat ttgatatcaa caggtattaa cttcgcgtca agcagcatca tttcgccatg
      241 ataaatcatc tcaccgtccg ggttatacat ggcgatacca tactcagaag gtggagtaac
      301 catattcgcg aaagcataaa ccgtcgtaac accagggttc gttcccctga caattacatg
      361 aagccttagt gcatgatatc catcaatctg ttcatgcgtg tacatgacat cggccttctt
      421 ctctgttctg ataaagaaaa aacaactttt gcctgacggg attgat
//
LOCUS       DAZKJR010000524          459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZKJR010000524 DAZKJR010000000
VERSION     DAZKJR010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.527"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>459
                     /locus_tag="ACHOWS_005566"
     CDS             <1..>459
                     /locus_tag="ACHOWS_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFQ0050950.1"
                     /translation="WKQRHAEGYASVYGGYKGKESQNIFFVPLMTDENGANVQTNDPA
                     EDPDLEAVGYYGSKWRNDQKTWTSVDRASHFSSWARRGIISDRLATAILVHAGRTAEF
                     ITGKQPDTVKPTGPSGEGPEREPEAPVSNRTLMSLLASGEDLASQGWRYY"
BASE COUNT          112 a          111 c          144 g           92 t
ORIGIN      
        1 tctggaagca gcgtcatgcg gaagggtatg catcagtata cggagggtat aagggtaagg
       61 agtcgcagaa cattttcttt gtaccgttaa tgacggatga gaatggtgcg aatgtgcaga
      121 ccaatgaccc ggcagaagac ccggacctgg aagccgtcgg ttattacggt tcaaagtggc
      181 gtaatgacca gaagacctgg acatctgtgg acagggccag ccatttcagt tcatgggctc
      241 gccgtgggat tatttccgac cgtctggcaa cggcgattct ggtgcatgcc gggagaaccg
      301 ctgaattcat taccggaaaa cagcctgata cggtgaagcc caccggacct tccggtgaag
      361 gcccggagag agagccggaa gccccggtca gtaaccgaac cctgatgagt ctgctggcgt
      421 ccggtgaaga cctggcatca cagggctggc gctattatc
//
LOCUS       DAZKJR010000525          456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZKJR010000525 DAZKJR010000000
VERSION     DAZKJR010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.528"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>456
                     /locus_tag="ACHOWS_005567"
     CDS             <1..>456
                     /locus_tag="ACHOWS_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0050951.1"
                     /translation="KDTLLTRMNDLNRDLVNYSHDNTRTSVGARLAMYEVGLKTYSPI
                     GQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDLIDTLSTRGIPGVALTILAFSAI
                     FIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAY"
BASE COUNT          142 a           81 c           82 g          151 t
ORIGIN      
        1 taaagataca ttacttacga gaatgaatga cttaaatagg gatttagtta attattcgca
       61 tgataacacc agaacttcag tcggtgcccg tctggcaatg tatgaagttg gtttaaaaac
      121 atattctcca ataggacaat cactggaaaa acgtgcggaa aaaatacatg aactagaaga
      181 gaaagagcct agattaagtg gcgctttacc ctttgtagat tctcatttgc ataacgatct
      241 catagatacg ttatcaacgc gtggtattcc tggagtagca ttaacaattt tagccttttc
      301 agcaatattc atatatgcct taagaactgc taaagaacct tatattttaa tcttgctttt
      361 ttcactactg gtagtaggcc taagtgatgt aatactcttt tctaaaccgg ttccgactgc
      421 tgtgtttgtc accataatat tgctttgtgc ttattt
//
LOCUS       DAZKJR010000526          445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZKJR010000526 DAZKJR010000000
VERSION     DAZKJR010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.529"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            78..440
                     /locus_tag="ACHOWS_005568"
     CDS             78..440
                     /locus_tag="ACHOWS_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050952.1"
                     /translation="MKKYILVSLMGLSLNAFAGGSGWNPDRVDPSRCVMATGSGPTSA
                     GYMVYRTTDSVCMQGINEGKVKGVHIYDGHHEFNDGTVDTFSGNVTPISPLTLKTDVQ
                     KVNKVGIKRWWYKASWIK"
BASE COUNT          140 a           68 c          113 g          124 t
ORIGIN      
        1 gactgaggga gcgtaagtgc agtacatgca acttgttgca tgacaaatat attatctatt
       61 aacaataaga ggttataatg aagaaatata ttttagtatc tctgatggga ttaagtttga
      121 acgcatttgc gggtggcagc ggctggaacc cagacagggt agacccgagc cgttgtgtta
      181 tggcgacggg gtcaggtcct acctctgcgg gttatatggt ataccgaacg actgactcag
      241 tgtgtatgca gggtataaat gaaggaaaag tgaaaggtgt ccatatttat gatgggcatc
      301 atgagttcaa tgatggtact gttgacacgt ttagcggtaa tgttacccca atatcacctt
      361 tgacattaaa aactgatgtt caaaaagtta ataaagttgg catcaaacgc tggtggtata
      421 aagctagctg gattaaataa aaaat
//
LOCUS       DAZKJR010000527          442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZKJR010000527 DAZKJR010000000
VERSION     DAZKJR010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.530"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          129 a           91 c          101 g          121 t
ORIGIN      
        1 ttgacaggaa cacattcaaa aaaatttctt tattcatttc aggagcgatt ttcatctgtt
       61 acttgaagta tctgtgagat taagtgactc cccctaacgt cgtactaaat agtaattact
      121 gtccataccg ggagcagtat tcggcgctcc ccacatgcag cagaataacc gcatcagcga
      181 agatgtattg tatttttact atcacaatgt gtacggacgg tttatggaaa agaatgaaaa
      241 ttagatacgc gatggggcgg caggttacac ggttatcact acgatagcca atactggctg
      301 gtgcattacc gccgtgaaca ataggacgcc gtactgctgg aaatttctta cctttatgac
      361 tccccgcgcc gccgtattgg gaaacgggaa gcaatcaaga tgaggctgat ataatgtgcg
      421 gaatgcctga gtatttttag aa
//
LOCUS       DAZKJR010000528          440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZKJR010000528 DAZKJR010000000
VERSION     DAZKJR010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.531"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..218)
                     /locus_tag="ACHOWS_005569"
     CDS             complement(<1..218)
                     /locus_tag="ACHOWS_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFQ0050953.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQL"
     gene            complement(307..>440)
                     /locus_tag="ACHOWS_005570"
     CDS             complement(307..>440)
                     /locus_tag="ACHOWS_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050954.1"
                     /translation="SAGGQCRVERCFRVARPGSSGQYVALKTAVQSLMPRGIATIIR"
BASE COUNT          118 a          115 c          102 g          105 t
ORIGIN      
        1 atcagttgtg gtgtacgggt aagcaacaag cgaaaaggca gttcaaaact ggtgacgtaa
       61 tccacattca gcagggcaat gcgaagtcgt tcttctggtc cggcttctgt ctgccggcac
      121 tcctccagga catcctgcca ctgcaggcga agacgggaag actcattcag ttctgtaaag
      181 cagtatttat ccgccagata gtcaattcgt gtatgcatac tgaagagtat tccgtataaa
      241 gattcagctg gcaaaacttt atcagtctgt aaaaactaac ggaagagtcg atatttctcc
      301 cgtccatcac cggatgattg ttgcaatacc ccgtggcatc agagactgaa cagccgtttt
      361 taacgccacg tattgccctg atgaaccagg ccgggcaacc cgaaaacacc tttccacccg
      421 gcattgtccg ccagcgctta
//
LOCUS       DAZKJR010000529          430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZKJR010000529 DAZKJR010000000
VERSION     DAZKJR010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.532"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>430)
                     /locus_tag="ACHOWS_005571"
     CDS             complement(<1..>430)
                     /locus_tag="ACHOWS_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HFQ0050955.1"
                     /translation="SQEERLSLISRANEALSLWDNWLHPFTSGSETGDDPAYDDNFQL
                     MREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRDGERGLSEGLLLLV
                     AMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGV"
BASE COUNT          101 a          128 c          109 g           92 t
ORIGIN      
        1 tccaccccgg gccataacag cagtgcatca gtcacttttg cactgttcag ccactccagc
       61 gcagctttcc gggcggcagg acgccgggga tgacatgcct gtccgtagcg ggtcagcatc
      121 gcaacaagaa gtaataagcc ttcggacaac cctctttcac catcccgggt gagacgggcc
      181 agcacgtacc aggtcacgac acgaatatct ctggcgcatt cgcaaaggca cttctgagcc
      241 agttcgcaca acagcgctga atcagtacct gaaatcttat tgatctcttc acgcatcagc
      301 tgaaaattat catcgtatgc aggatcgtcc ccggtttcag atccggaggt aaatggatgc
      361 agccagttat cccagagcga cagggcctca tttgcgcggg aaatgaggct cagcctttct
      421 tcctggctgg
//
LOCUS       DAZKJR010000530          429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZKJR010000530 DAZKJR010000000
VERSION     DAZKJR010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.533"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            230..>429
                     /locus_tag="ACHOWS_005572"
     CDS             230..>429
                     /locus_tag="ACHOWS_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFQ0050956.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSGYMEAGDDSICYAALDDG"
BASE COUNT          117 a           95 c          107 g          110 t
ORIGIN      
        1 caccggaaag gtggcggggg actcacaacg ataatatctg tctgaaatta ttttgaatgt
       61 acccggtgca ctgtaaaaga aaggcagtgt tccgttcctc ctccctgtca tgaataacct
      121 ggtgtatctc acaatatctg ctgacttgct ccggggctgc ctgaaccaca agattgctgt
      181 agtattgccc ccggagattc agttccagac caggaaaaac aggttaaaaa tgacaagtat
      241 tcaacaaaga gcagaactgc accgccagat atggcaaatt gctaacgatg tcagaggttc
      301 ggtcgatgga tgggatttta agcaatacgt tctgggcgcg cttttctacc gttttatcag
      361 cgaaaatttc tccgggtata tggaagccgg tgatgacagc atctgttatg cggcacttga
      421 tgacggcat
//
LOCUS       DAZKJR010000531          427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZKJR010000531 DAZKJR010000000
VERSION     DAZKJR010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.534"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..200)
                     /locus_tag="ACHOWS_005573"
     CDS             complement(<1..200)
                     /locus_tag="ACHOWS_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFQ0050957.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSHMENGDDSICYAKLDDNV"
BASE COUNT          107 a           98 c           94 g          128 t
ORIGIN      
        1 acgttgtcat ccagttttgc ataacagatg ctgtcatcac cattttccat atggctggaa
       61 aaattttcgc tgataaaacg gtagaaaagt gcgcccagaa cgtattgctt aaaatcccat
      121 ccatcaacag aacctctgac atcattggca atttgccata tctggcggtg cagttctgct
      181 cgttgttgaa tacttgtcat ttttaatcca ttttcctggt ctggacctga atcattgggg
      241 gcaattctac agcaatcttg tggttcaggc agccccggag taagtcggtg tacattgtga
      301 gatacctggg gtattcacga caaggaggag gaacggaata ctgcctttcc tttgcagtgc
      361 accaggtaca ttctaaataa tttcagacag atattatcgt tgtgagtccc ccgcctcctt
      421 tccggtg
//
LOCUS       DAZKJR010000532          422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZKJR010000532 DAZKJR010000000
VERSION     DAZKJR010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.535"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          115 a          114 c           88 g          105 t
ORIGIN      
        1 tgagttatat ttttctgtat atatgacact ttggttccca ctacttatta tttggtggac
       61 acaactttgt cagatgcttc agattatgac cagacaactt tcactgtacg tctaaaagga
      121 tttaccttct cttggtaagt gatacctgca gtcgggagtt aaacgcctga cgacaggaaa
      181 tcagccaggc cagtaaccac acgggctgac agcgaatcag aaccagaatc agcccagagt
      241 cgttcccggg gcagttctca ccaatacccg tgtcaacact tcgcaacctg acgcaattag
      301 agcttcagag ccaacctccg gtacctgccc agcgatcgct tctgtgcaac aaaataaatc
      361 atccatcgtc agagggcgat cattcgtatg tttcaccgat ggccccccga aaacgcatca
      421 gg
//
LOCUS       DAZKJR010000533          416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZKJR010000533 DAZKJR010000000
VERSION     DAZKJR010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.536"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(115..>416)
                     /locus_tag="ACHOWS_005574"
     CDS             complement(115..>416)
                     /locus_tag="ACHOWS_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050958.1"
                     /translation="NELKGSWISGPTLTSGSSWNNACEENGVTFSLSEDNFSIDPVLG
                     YTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKEEVRKESPGNG"
BASE COUNT          102 a          106 c          111 g           97 t
ORIGIN      
        1 aagccggtgg ccgaagcgac gttttccgga aaggcataaa aaagaaatgc cacgatgacc
       61 gtcagaaaaa aggcgacagt cacaacagag ccgatgagtg ttttaacttt taccttagcc
      121 attcccgggt gattccttgc gaacttcttc tttagtgatg acggcaaccc agacgtcgtc
      181 tttgtcagct gtgggctggg cggcgataac ctgcgcacct cgttgttcaa aggcagcatc
      241 agcaagcgtg atggcaagcc atggagcagc atggccggta tagcccagca cgggatcgat
      301 actgaaattg tcttctgaaa ggctgaatgt cactccgttc tcttcgcagg cattattcca
      361 gctgctgccc gacgtcaatg tgggaccgga gatccagctt cctttaagct cattgg
//
LOCUS       DAZKJR010000534          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZKJR010000534 DAZKJR010000000
VERSION     DAZKJR010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.537"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>415)
                     /locus_tag="ACHOWS_005575"
     CDS             complement(<1..>415)
                     /locus_tag="ACHOWS_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFQ0050959.1"
                     /translation="MFPDAVLSLLAPFASTVQGGQRTRRLILLIWLCVLTALGISALN
                     NRDLIRQVSTDLQRWNAVPKGHYRPEAESLAALKQDALLLEQWQRQGVPLRYSLGYYP
                     GQRLWLALQQAIDTRVPPPSPEPEPVPEIVRLDSLA"
BASE COUNT           96 a          126 c          118 g           75 t
ORIGIN      
        1 gccaggctat cgagtcgtac aatttccggc accggttctg gttcagggga tggtggtgga
       61 acccgggtat cgatagcctg ctgcagggcc agccacagac gttgtcccgg gtaatagccc
      121 aggctgtaac gtaaaggcac tccctgacgc tgccactgct caagcagaag cgcatcctgt
      181 ttcagtgcgg ccagcgattc cgcctcagga cggtagtgcc ccttcgggac agcattccag
      241 cgctgtaaat cggtgcttac ctggcggatc agatcacgat tgttaagcgc agaaatcccc
      301 agcgcagtca ggacacacag ccatatcagc aaaatcagac gacgggtacg ctggcctccc
      361 tgtaccgtcg acgcaaaagg tgccagcagc gacagaaccg catccgggaa catcc
//
LOCUS       DAZKJR010000535          414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZKJR010000535 DAZKJR010000000
VERSION     DAZKJR010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.538"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>414)
                     /locus_tag="ACHOWS_005576"
     CDS             complement(<1..>414)
                     /locus_tag="ACHOWS_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050960.1"
                     /translation="QFQAIREVIRPQQEVTPEAQSQPENAEDGNGSIYPTVRFRDPNE
                     VRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGSDVFRNAAATGLKPSVVKKGENQ
                     YAVEMDNPAFSEDVATETINTLADGERIADADPME"
BASE COUNT           77 a          123 c           98 g          116 t
ORIGIN      
        1 tgctccatcg ggtcagcatc agcaatacgc tctccgtcag ccagggtgtt aatggtttcc
       61 gttgccacat cttcagagaa cgcaggatta tccatttcaa cggcatactg attctcgcct
      121 ttcttcacta cggacggttt caggccggta gcggcggcat tacggaaaac atcactcccc
      181 atggcgcttt tctcatccgt gaaataacgg ttgtccgggc gcaccttttc aatgcgttcc
      241 gctggtctgg acgcaccatt cccgttaatt tcaatgcgga cttcattcgg gtcccggaac
      301 cgcaccgtcg ggtaaatgct cccgttacca tcctccgcat tttcaggctg tgactgtgct
      361 tccggcgtca cttcctgttg tgggcgaatg acttctcgta ttgcctgaaa ctgt
//
LOCUS       DAZKJR010000536          413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZKJR010000536 DAZKJR010000000
VERSION     DAZKJR010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.539"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..200)
                     /locus_tag="ACHOWS_005577"
     CDS             complement(<1..200)
                     /locus_tag="ACHOWS_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFQ0050961.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDD"
     gene            complement(234..>413)
                     /locus_tag="ACHOWS_005578"
     CDS             complement(234..>413)
                     /locus_tag="ACHOWS_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFQ0050962.1"
                     /translation="TLEDWEPALLFEVRARRLKLLRMKAGRSESDKARLMPEMDTLLA
                     GLIAIDPARAMVLCG"
BASE COUNT          101 a          130 c          101 g           81 t
ORIGIN      
        1 tcatcatcca gcttttcacg caaacgtccc atcaggaagg caaagccttc aaacagacgt
       61 tccacaccgg gatcgcaggc gccaggctta tccagattca gcattgctgc ccgatccgga
      121 tgagcacggg caaactcttt accggcttcc cgcagatagc gcatctctgc ttcgtaatag
      181 cgcagggtta aatcatccat gcacggcatt cctgaaaatg attaaagaga acatcagcca
      241 cagagcacca tggcacgggc cgggtcaatg gcgatcagcc ccgccagcag cgtatccatc
      301 tcaggcatca gacgggcttt atcagattcg ctgcgaccgg ctttcatccg tagcagcttc
      361 aggcgtcggg cccggacttc aaacagcaaa gcgggctccc agtcttccag cgt
//
LOCUS       DAZKJR010000537          404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZKJR010000537 DAZKJR010000000
VERSION     DAZKJR010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.540"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>404
                     /locus_tag="ACHOWS_005579"
     CDS             <1..>404
                     /locus_tag="ACHOWS_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFQ0050963.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKV"
BASE COUNT           76 a          121 c          115 g           92 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcagc cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggcggt
      301 cagattggta ctttcgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
      361 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag taca
//
LOCUS       DAZKJR010000538          397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZKJR010000538 DAZKJR010000000
VERSION     DAZKJR010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.541"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>397
                     /locus_tag="ACHOWS_005580"
     CDS             <1..>397
                     /locus_tag="ACHOWS_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFQ0050964.1"
                     /translation="HGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTINKNGRQ
                     VIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGWQVIKE
                     GGTAAHTTINQKGKLQVNAGGKASDVTQNT"
BASE COUNT          110 a          100 c          127 g           60 t
ORIGIN      
        1 acatggcgga ctggcaaccg gaaccatcgt caacaccggt gcagaaggtg gtccggagtc
       61 tgaaaatgtg tccagcggtc agatggtcgg agggacggct gaatccacca ccatcaacaa
      121 aaatggccgg caggttatct ggtcttcggg gatggcacgg gacaccctca tttacgccgg
      181 tggtgaccag acggtacacg gagaggcaca taacacccgg ctggaggggg gtaaccagta
      241 tgtacacaac ggtggcacgg caacagagac gctgataaac cgtgatggct ggcaggtgat
      301 taaggaagga ggaactgccg cgcataccac catcaaccag aaaggaaagc tgcaggtgaa
      361 tgccggcggt aaagcgtctg atgtcaccca gaacacg
//
LOCUS       DAZKJR010000539          397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZKJR010000539 DAZKJR010000000
VERSION     DAZKJR010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.542"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>397
                     /locus_tag="ACHOWS_005581"
     CDS             <1..>397
                     /locus_tag="ACHOWS_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFQ0050965.1"
                     /translation="LMLARPDKADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFY
                     AQNLPAHPEWKIKPDDELISTVRQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLAD
                     MTGDTDASTVFSTDEVVPGMFTRQAWEEQV"
BASE COUNT          101 a           96 c          117 g           83 t
ORIGIN      
        1 atctgatgct ggcgcgcccg gacaaagcgg acgccggctg gctggcaaaa aatgcgctga
       61 aagccttacc gaaacgccgg aatgtgccgg atggcatatg gcagaacgtc gcaccgaaac
      121 tgctggggtt ttatgcgcaa aaccttccgg ctcatccgga atggaaaatt aagcctgacg
      181 atgaattaat cagtacggtc cgtcagattc tgcttaagca aattggccag cggaatgctg
      241 agtccgggtt atatcaggag atgcttaagc gcgtggccag taactggcct gatttaacac
      301 ttgcggatat gaccggtgac accgatgcct caacggtatt cagcactgac gaagtcgttc
      361 cgggaatgtt tacgcgtcag gcctgggaag agcaggt
//
LOCUS       DAZKJR010000540          395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZKJR010000540 DAZKJR010000000
VERSION     DAZKJR010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.543"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>395
                     /locus_tag="ACHOWS_005582"
     CDS             <1..>395
                     /locus_tag="ACHOWS_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFQ0050966.1"
                     /translation="IFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVGTTDTLR
                     EMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTPWLNTAP
                     GLFIDGYTSAAASTVPSLSRTLIYDYEQNP"
BASE COUNT           93 a           97 c          108 g           97 t
ORIGIN      
        1 attttctggc ggacacacca ccgcggtcac cgtaactggc tggcactgct gctgttcgta
       61 ttatgctctg taaacagctg gccgttgcgg atggttaaag gaaccgttgt ggggacaact
      121 gacacattgc gtgaaatgca gcgttatgaa caactgagtc agcacagggc tgacagctgg
      181 aaaatcctgc cgggagcacc gttgtatgac acgattgtga ttgtcactgg tgagagtgtg
      241 cgcagggatt atatgtcggt atatggttat cccgtaccaa ccacaccgtg gctgaatacg
      301 gctcccggtt tatttattga cggctataca tcggcagcag ccagtaccgt accttccctg
      361 agccggacac tgatttatga ctatgagcag aaccc
//
LOCUS       DAZKJR010000541          387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZKJR010000541 DAZKJR010000000
VERSION     DAZKJR010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.544"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..>387
                     /gene="tssB"
                     /locus_tag="ACHOWS_005583"
     CDS             23..>387
                     /gene="tssB"
                     /locus_tag="ACHOWS_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFQ0050967.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PEQVARQIPRLKAMLAMRSL"
BASE COUNT          107 a           87 c          105 g           88 t
ORIGIN      
        1 gcaggtttca caggagaacg tcatgagcag ttcgtttcag aatgagatcc cgaaggcaag
       61 ggtgaatctg aaattagacc tacatacagg tggtgggcag aagaaagtgg agcttccgct
      121 caaactgctg gttgccggtg atttcagtaa cggacaggaa gagcgcccgc tgtcagagcg
      181 cataaaaatg gatgttaata aaaataactt taatgctgtt ctggctgata tctcgccgtc
      241 agtacgcctg aatgtggaga acacacttgc cggtgatggt actgaagaga gcatcagcct
      301 gactttccgt aatatgaacg atttcacgcc ggagcaggtt gcccgacaaa ttccccgtct
      361 taaagccatg ctggccatgc gcagcct
//
LOCUS       DAZKJR010000542          379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZKJR010000542 DAZKJR010000000
VERSION     DAZKJR010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.545"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>379
                     /locus_tag="ACHOWS_005584"
     CDS             <1..>379
                     /locus_tag="ACHOWS_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050968.1"
                     /translation="YRINRFGRWEKYYYIQLRGAFLSAIHHQIFKNQLDTETITVSYE
                     FILCQHLITNTEFSYLALPENYNRLFLPNSKNKTNNGLKTLNSEAVGRLLAAGGVYNG
                     NIEGFRDTAEKLGGDAIKGYDQIL"
BASE COUNT          135 a           68 c           70 g          106 t
ORIGIN      
        1 ctatcgaata aatagatttg gcagatggga aaagtattat tatatacaac taagaggcgc
       61 ttttttatcg gctattcatc accagatctt taaaaaccaa ctggatacag aaacaataac
      121 tgttagttat gaatttatcc tctgccaaca tcttatcact aacaccgagt tcagctattt
      181 ggcactccct gaaaattata accgcttgtt tttaccaaat tcaaaaaaca aaacaaataa
      241 tggtttaaaa acgttaaaca gcgaagctgt tggcaggcta cttgctgctg gtggcgtata
      301 caatgggaac attgaaggtt tcagagatac tgcggaaaaa ctgggtggag atgcaatcaa
      361 aggctatgat caaatacta
//
LOCUS       DAZKJR010000543          377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZKJR010000543 DAZKJR010000000
VERSION     DAZKJR010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.546"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>377)
                     /locus_tag="ACHOWS_005585"
     CDS             complement(<1..>377)
                     /locus_tag="ACHOWS_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0050969.1"
                     /translation="YVKHARWVELWRDCLRVAYDPNVDIRVVKSRKPKDDESLPCVVA
                     EQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGALKNVLQLERETNE
                     DLIMADGMAEGTDDGSRLAFSWVP"
BASE COUNT           93 a          122 c           75 g           87 t
ORIGIN      
        1 ggcacccacg aaaacgccag acgggaaccg tcatctgtac cctctgccat gccatccgcc
       61 atgattaaat cctcgttcgt ttcccgctcc agttgcagaa cgttcttaag cgctcccccc
      121 gtagcaacaa aacgacgctt gtgtacctgc cgtgttaatt cgaggaacca ttcaggatca
      181 gccagcatat cagcaggctt tgtggaatat ttcagagttt caaccaccgc gccgcgcagc
      241 tgttccgcaa caacacaagg taaagattca tcgtcttttg gtttccgtga cttcaccact
      301 ctgatatcca cattcgggtc atacgccacc cgcagacaat cacgccacag ttccacccaa
      361 cgggcatgtt tcacata
//
LOCUS       DAZKJR010000544          376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZKJR010000544 DAZKJR010000000
VERSION     DAZKJR010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.547"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>376
                     /locus_tag="ACHOWS_005586"
     CDS             <1..>376
                     /locus_tag="ACHOWS_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFQ0050970.1"
                     /translation="GWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIR
                     YRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEGGLKRVVKKEHA
                     DGSVTQSQFDAVGRLKAQTDAAG"
BASE COUNT           93 a          103 c          129 g           51 t
ORIGIN      
        1 ggctggacgc cgcgcggcga actggcggcg gtgtatgacc gcagcaatac acaggtgcgc
       61 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg
      121 ccggaaatcc gttaccgtta cgacagtgac gggcgggtga cagaacagct aaacccggca
      181 ggcttaagct acacgtatca gtatgagaaa gaccacatca ccatcaccga cagcctggac
      241 cgccgtgaag tgctgcacac gcagggcgaa ggcgggctga agcgggtggt gaaaaaggaa
      301 cacgcggacg gcagcgtcac gcagagtcag tttgacgcgg tgggcaggct taaggcacag
      361 acggatgccg caggcc
//
LOCUS       DAZKJR010000545          375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZKJR010000545 DAZKJR010000000
VERSION     DAZKJR010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.548"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>375
                     /locus_tag="ACHOWS_005587"
     CDS             <1..>375
                     /locus_tag="ACHOWS_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFQ0050971.1"
                     /translation="KQYGDFENGIPVHDTIARVVSCISPSKFHECFINWMRDCHTSDD
                     KDVIAIDGKTLRHSYDRSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEK"
BASE COUNT          119 a           72 c           84 g          100 t
ORIGIN      
        1 aaacaatatg gtgattttga aaatggtatt cctgttcacg ataccatcgc cagagttgta
       61 tcctgtatca gtccttcaaa atttcacgag tgctttatta actggatgcg agattgccat
      121 acttcagatg ataaagacgt tattgcaatc gatggaaaaa cactccggca ttcttatgac
      181 aggagtcgcc gcaggggagc gattcatgtc attagtgcgt tctcaacaat gcacagtctg
      241 gtcatcggac agatcaggac ggatgagaaa tctaatgaga ttacagctat cccagaactt
      301 cttaacatgc tggatattaa aggaaaaatc atcacaactg atgcgatggg ttgccagaaa
      361 gatattgcag agaag
//
LOCUS       DAZKJR010000546          374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZKJR010000546 DAZKJR010000000
VERSION     DAZKJR010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.549"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..100
                     /locus_tag="ACHOWS_005588"
     tRNA            24..100
                     /locus_tag="ACHOWS_005588"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHOWS_005589"
     tRNA            143..218
                     /locus_tag="ACHOWS_005589"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT          108 a           79 c           90 g           97 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
      361 caacacgatg atga
//
LOCUS       DAZKJR010000547          374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZKJR010000547 DAZKJR010000000
VERSION     DAZKJR010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.550"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>374
                     /locus_tag="ACHOWS_005590"
     CDS             <1..>374
                     /locus_tag="ACHOWS_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFQ0050972.1"
                     /translation="LHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQD
                     FEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASA
                     HMPFIGSVGPAFFRKDTMEEVAA"
BASE COUNT           77 a           81 c          116 g          100 t
ORIGIN      
        1 cttcatcatc cggaatttca gaaagtggag tccctgtggc ggggcgtgaa gtctctggtg
       61 gataaaacgg acttccgccg taatgtgaaa attgaactgc tggatctgtc caaagacgat
      121 ctgcgtcagg attttgagga tgcgccggaa attattcaga gtggtcttta tctgcagact
      181 tatgtggcgg agtacgatac gccgggtggt gaacccattg cagccctgat ttctgcctgg
      241 gagtttgatg cgtctgcgca ggatgtggcg ctgctgaaaa atatttcccg ggtggctgcg
      301 tccgcgcata tgccatttat tggttcagtt ggcccggctt tcttccggaa ggatacgatg
      361 gaagaggtgg ccgc
//
LOCUS       DAZKJR010000548          363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZKJR010000548 DAZKJR010000000
VERSION     DAZKJR010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.551"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>363
                     /locus_tag="ACHOWS_005591"
     CDS             <1..>363
                     /locus_tag="ACHOWS_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0050973.1"
                     /translation="LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSR
                     HSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGE
                     RGALLYSLIGTCKLNDVDP"
BASE COUNT           91 a           86 c          115 g           71 t
ORIGIN      
        1 gttgtacgcc atagaggcgg atataagggg aatgccggca gagcagcggc ttgctgaacg
       61 tcagcgaaaa acgaaaccgc tgttgaaatc cctggaaagc tggttgcgtg aaaagatgaa
      121 gaccctgtcg cgacactcag agttggcgaa ggcgttcgcg tacgcactta accagtggcc
      181 ggcactgacg tactatgcga acgatggctg ggtggaaatc gacaacaaca tcgctgaaaa
      241 tgccctgcgg gcggtcagtc tgggtcgtaa aaacttcctg ttcttcggct ctgaccatgg
      301 tggtgagcgg ggagcgctac tgtacagcct gatcgggacg tgcaaactga atgacgtgga
      361 tcc
//
LOCUS       DAZKJR010000549          360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZKJR010000549 DAZKJR010000000
VERSION     DAZKJR010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.552"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..>360)
                     /locus_tag="ACHOWS_005592"
     CDS             complement(59..>360)
                     /locus_tag="ACHOWS_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFQ0050974.1"
                     /translation="EAEGWLPGGVLHTDLLVLLRVYLGYRSDVRLRLTVPVRLLPEPR
                     LGKGRPVQLGRTGLLGLKAGKLSNGRESLTVSLGCYEGLKCAALPSAEDGHYRFE"
BASE COUNT           84 a          115 c           96 g           65 t
ORIGIN      
        1 cccaccagcg gattactgtt accattcagg cgttccttta ttgagagaga acaggtgatt
       61 attcgaagcg ataatgcccg tcttcagcag acggtagcgc agcacatttc agcccttcgt
      121 agcaacccag actgaccgtc agactctccc ggccattgct gagtttgccc gctttcagcc
      181 ccagtaaacc ggtacggccc agctgaacag gcctgccttt gcccaggcgg ggctcaggca
      241 acaaccgcac ggggacagta aggcgcagcc ggacgtcact gcggtagccg agataaacgc
      301 gcagcaggac cagcaaatcg gtatggagaa cacctccggg caaccagcct tccgcctcgt
//
LOCUS       DAZKJR010000550          358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZKJR010000550 DAZKJR010000000
VERSION     DAZKJR010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.553"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          116 a          114 c           66 g           62 t
ORIGIN      
        1 gatattacgg catcacatac ccaccccaaa acgggcacgt ctccgcaggc cgcacagttc
       61 agccaggtcg ctcaggagtg ccgccagctt aaaaataaat attcaccaat aatcgaataa
      121 tcattcactt taacaggccg cgcaatgtgg ccttttttat tcccggacga taaaaaatat
      181 tcatcaacgg acatcaccca ccacgaaaaa ccgtccgcag cagaaaaaaa gatggacgaa
      241 acgccccacg taatccccgc gtaaaacgtc acgtaacgcc acgtaacgaa gagataaccc
      301 cgccagcccg attcgatccc ctgacatcag aaaacgattt ctgcgcccca gaatcgcc
//
LOCUS       DAZKJR010000551          354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZKJR010000551 DAZKJR010000000
VERSION     DAZKJR010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.554"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..251)
                     /locus_tag="ACHOWS_005593"
     CDS             complement(<1..251)
                     /locus_tag="ACHOWS_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HFQ0050975.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDVEYIFVAEKIVQYAEKNL"
BASE COUNT          107 a           74 c           55 g          118 t
ORIGIN      
        1 tcgagatttt tctccgcata ttgcactatt ttttcggcaa caaaaatata ctcaacatcc
       61 acatgttcaa tcaatttgta gagtttattt ttttgttcgt ttctgacata aaaaatccgg
      121 ttagccggat gttcagggac cgacattcca tattttttgt tgtaaccgac gccaggtcca
      181 gaaatgataa cctcctgtcc attcatcgat gcctgtacac aattattgtt catgactttt
      241 tcgatgatca ttccatgact ccaaaaaaaa aggcaaggca cgtcagaagt aataattact
      301 tcaaacattg ccttgcctga tttaacagtt acaagatgat taactaatgc gtat
//
LOCUS       DAZKJR010000552          351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZKJR010000552 DAZKJR010000000
VERSION     DAZKJR010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.555"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..>351
                     /locus_tag="ACHOWS_005594"
     CDS             71..>351
                     /locus_tag="ACHOWS_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFQ0050976.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVL"
BASE COUNT           79 a           73 c           74 g          125 t
ORIGIN      
        1 aaagatacga atagcattta cacaataccc ggaatagact ttcgtcactt ttaattaaag
       61 ggatgttttt atgcagtggt atttagccgt attaaaaaat tatgttggtt tctctggtcg
      121 cgcacgtcgt aaagagtact ggatgtttac tctgattaac gccatcgtcg gcgctattat
      181 caatgtcatt caattgattt taggtttaga gtttccattc ctttctctca tttacctggc
      241 agcaacaatc attccggtca ttgctctgtg cgttcgtcgt ctgcacgata ctgaccgttc
      301 aggtgcgtgg gcgcttctct atctggttcc gatcattggc tggttagttc t
//
LOCUS       DAZKJR010000553          350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZKJR010000553 DAZKJR010000000
VERSION     DAZKJR010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.556"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>350)
                     /locus_tag="ACHOWS_005595"
     CDS             complement(<1..>350)
                     /locus_tag="ACHOWS_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050977.1"
                     /translation="RFRGFTGDDTEVQARLARGNAPMAEELVRSQMAEGDAGPTAQEL
                     TERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAPAP
                     ENAAGRQGETLEGE"
BASE COUNT           61 a          124 c           93 g           72 t
ORIGIN      
        1 atttcaccct cgagtgtttc cccctggcga ccagcggcgt tttccggtgc cggagcacgt
       61 cctttctgga aactctggcc cttaacctca ccggttgtgg taaaacgacc accacgtccg
      121 gcctgattct catccggcgt acgcgccacc tctcccggta acggatatcc ctgtccggga
      181 tgaatatcgc cgggggcggg cagacgcgga cgctcagtta actcctgtgc tgtcggaccg
      241 gcatcaccct cagccatctg tgaacgtaca agctcctccg ccatcggcgc gttgccacgg
      301 gcaaggcggg cctgtacctc cgtatcatcc cccgtgaaac cacggaatcg
//
LOCUS       DAZKJR010000554          350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZKJR010000554 DAZKJR010000000
VERSION     DAZKJR010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.557"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>350)
                     /locus_tag="ACHOWS_005596"
     CDS             complement(<1..>350)
                     /locus_tag="ACHOWS_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050978.1"
                     /translation="RFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQEL
                     TERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAP
                     ENAAGRQGETLEGET"
BASE COUNT           59 a          120 c           93 g           78 t
ORIGIN      
        1 gtttcaccct cgagtgtttc cccctggcga ccagcggcgt tttccggtgc cggagcttgt
       61 cctttctgga aactctggcc cttaacctca ccggttgtgg taaaacgacc accacgtcct
      121 gcctgattct catccggcgt acgcgccacc tctcccggta acggatatcc ctgtccggga
      181 tgaatatcgc cgggagcggg caggcgtgga cgctcagtta actcctgtgc tgtcggaccg
      241 gcatcacctt cagccatctg tgaacgtaca agctcctctg ccgtcggcac gttgccacgg
      301 gcaaggcggg cctgtacctc cgtatcatcc cccgtgaaac cacggaatcg
//
LOCUS       DAZKJR010000555          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZKJR010000555 DAZKJR010000000
VERSION     DAZKJR010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.558"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>344
                     /locus_tag="ACHOWS_005597"
     CDS             <1..>344
                     /locus_tag="ACHOWS_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFQ0050979.1"
                     /translation="SGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKA
                     KVWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSA
                     SDARKYIDSHRD"
BASE COUNT          106 a           66 c           97 g           75 t
ORIGIN      
        1 tcgggtgtgt atcaggcggc aggagggaag cgttacgatc cgccacagaa actttattca
       61 ctgcgcaata aggacaataa ggggagtaac ctctttttct cggaaagcag ggatgaatta
      121 ctggcaaagg cgaaagtctg gtttgcagag caggaggaaa aatcacaggc gaaaggtgat
      181 gaaaaaacag caccgtcacc ggatgacaaa atccgctttg acgtttaccg gaatacccgc
      241 agtggcgata tttttatcgc ttacggtaaa aacaaaatgc gggtgagagg tggctttaag
      301 tcagccagtg atgcgcgtaa gtacattgat tcacatcgtg atga
//
LOCUS       DAZKJR010000556          339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZKJR010000556 DAZKJR010000000
VERSION     DAZKJR010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.559"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>339
                     /locus_tag="ACHOWS_005598"
     CDS             <1..>339
                     /locus_tag="ACHOWS_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HFQ0050980.1"
                     /translation="FDIIFLGSDQIWNANYTNGVDPNYYGQGPYCKAHKLVSYAASMG
                     KLCLGKYEEQAFLSLINNIQQIGVRENYLKTYIEEKTDLKCDVNLDPTLLLTKSDWDK
                     LAAPNDTQERY"
BASE COUNT          120 a           50 c           68 g          101 t
ORIGIN      
        1 ttcgatatta tctttcttgg tagtgatcag atatggaatg caaattatac taacggagta
       61 gaccctaact attatggaca aggaccttat tgtaaagcgc acaaattagt ttcttatgcg
      121 gcaagtatgg gtaagctgtg tttgggaaaa tatgaggaac aagcattctt atctttgata
      181 aataatatac agcagattgg tgttagagaa aattatttaa agacgtacat agaagaaaaa
      241 accgatttaa aatgtgatgt taatcttgat ccgactcttc tgcttactaa aagcgattgg
      301 gataaactgg cagcacctaa cgatacacaa gaacgctat
//
LOCUS       DAZKJR010000557          338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZKJR010000557 DAZKJR010000000
VERSION     DAZKJR010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.560"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..>338
                     /locus_tag="ACHOWS_005599"
     CDS             7..>338
                     /locus_tag="ACHOWS_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050981.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDR"
BASE COUNT           84 a           84 c          123 g           47 t
ORIGIN      
        1 gacagagtgg gtaaaggggg cggcaagggg cacacaccgc gtgaggcgaa ggacaatctc
       61 aaatccacgc agatgatgag cgtgattgat gccattggtg agggaccggt ggaaggtccg
      121 gtgaagggac tgcagagtat tctggtgaac aaaaccccgc tgacggacac ggacggtaat
      181 cccgtgatac acggtgtgac cgccgtctgg cgtgccgggg agcaggagca gacaccgccg
      241 gaaggctttg agtcctccgg ggcagaaacc gcactgggcg tggaggtgac gaaggcaaag
      301 ccggtgacgc gcaccatcac gtcagcgaac attgaccg
//
LOCUS       DAZKJR010000558          336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZKJR010000558 DAZKJR010000000
VERSION     DAZKJR010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.561"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>336
                     /locus_tag="ACHOWS_005600"
     CDS             <1..>336
                     /locus_tag="ACHOWS_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0050982.1"
                     /translation="KQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGY
                     CSAQGEQWEYRYDASGRRTEKRCDRKKIRFTYLWDGDNIAEIREYRDDKLYSVRHLVF
                     NGFELISQQ"
BASE COUNT           88 a           89 c          109 g           50 t
ORIGIN      
        1 gaaacaggcc ggaaacacgc agtatgacta tgacgccgca ggccggatgg tcagccgcac
       61 cagacaccgt gacggctacc gcccggaaac agagcggttc cggtgggaca gccgtgacca
      121 gctgaccggg tattgcagcg cacagggtga gcagtgggaa taccgctacg acgccagcgg
      181 caggcggacg gaaaaacgct gcgaccggaa gaaaatccgt tttacgtacc tgtgggacgg
      241 cgacaatatt gcggaaatcc gggaataccg cgatgataaa ctgtacagcg tacggcacct
      301 ggtgtttaac ggctttgagc tgataagcca gcagtg
//
LOCUS       DAZKJR010000559          334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZKJR010000559 DAZKJR010000000
VERSION     DAZKJR010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.562"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>334)
                     /gene="tssK"
                     /locus_tag="ACHOWS_005601"
     CDS             complement(<1..>334)
                     /gene="tssK"
                     /locus_tag="ACHOWS_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFQ0050983.1"
                     /translation="WSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGVEQVEFND
                     SLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVVIALPHMQ
                     PGVANVEQ"
BASE COUNT           84 a          100 c           88 g           62 t
ORIGIN      
        1 tcctgctcaa cgttggcaac gcctggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgcggt cagccagctg accggcatct aactcacgtg gcgccggagg cagatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggacg acagaagact gtcattaaac tcaacctgtt ccactcccca gggaaacggg
      241 gatgccagcg cagagatacc ggcactgcga aaagactccc attcagcctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc caca
//
LOCUS       DAZKJR010000560          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZKJR010000560 DAZKJR010000000
VERSION     DAZKJR010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.563"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..252
                     /locus_tag="ACHOWS_005602"
     CDS             <1..252
                     /locus_tag="ACHOWS_005602"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFQ0050984.1"
                     /translation="QMRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAA
                     KANFAVAVGQYSQANAEKSISLGASARAIENNSTALGAK"
BASE COUNT          106 a           62 c           70 g           95 t
ORIGIN      
        1 caaatgcgta aaatcagtgt cagttatata tgcaaatcat tatttctcta ctgctcagct
       61 tctttacctg tcttatctgc aaatggtatt cctgataaaa tgatcgtgga acttggtgat
      121 aacagtgcgg caaaagctaa tttcgctgtt gctgttggtc aatattcaca ggcaaatgca
      181 gaaaaaagta tttctttagg tgcatcagca agagctattg aaaataactc taccgcgtta
      241 ggtgcaaagt aagcgtacag cctgaaccgt ctggtcagaa tctgacgaat tagacaaagt
      301 ggtgtccacc aaataagtag tgggaaccaa agt
//
LOCUS       DAZKJR010000561          324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZKJR010000561 DAZKJR010000000
VERSION     DAZKJR010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.564"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>324
                     /locus_tag="ACHOWS_005603"
     CDS             <1..>324
                     /locus_tag="ACHOWS_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFQ0050985.1"
                     /translation="VRTRRDEIDWVLTDKTHQTGSDISPEALKSRLTERYFTDFGNAW
                     LNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSVV
                     DSAKKL"
BASE COUNT           84 a           82 c           92 g           66 t
ORIGIN      
        1 gtcagaacac gccgcgatga aattgactgg gtactgacgg ataaaacaca tcagacaggg
       61 agcgatatct ctccggaggc cctgaagagc cgcctgactg aacgctattt taccgatttt
      121 ggtaatgcct ggctgaacat gctcaacagc atacagtggc atgaacccac gtcactttcc
      181 ggagccattg cccagttgag tctgctcggt gacgtccgcc agtctccgtt agttgcactg
      241 atgaacacgc tgaactacca ggggaaaacg gggcagaagg gggaggcact ggctgattca
      301 gtcgtggatt cagcgaaaaa gctg
//
LOCUS       DAZKJR010000562          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZKJR010000562 DAZKJR010000000
VERSION     DAZKJR010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.565"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>323
                     /locus_tag="ACHOWS_005604"
     CDS             <1..>323
                     /locus_tag="ACHOWS_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFQ0050986.1"
                     /translation="VRTRRDEIDWVLTDKTHQTGSDISPEALKIRLTERYFTDFGNAW
                     LNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSVV
                     DSAKKL"
BASE COUNT           82 a           80 c           92 g           69 t
ORIGIN      
        1 gtcagaacac gccgcgatga aattgactgg gtactgacgg ataagacgca tcagacaggg
       61 agcgatatct ctccggaggc gctgaagatc cgcctgactg agcgctattt taccgatttt
      121 ggcaatgcct ggctgaacat gctcaacagc atacagtggc atgaacccac gtcactttcc
      181 ggagccattg cccagttgag tctgctcggt gatgtccgtc agtctccgtt agttgcactg
      241 atgaacacgc tgaactacca ggggaaaacg gggcagaaag gtgaggcact ggctgattca
      301 gtcgtggatt cagcgaaaaa gct
//
LOCUS       DAZKJR010000563          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZKJR010000563 DAZKJR010000000
VERSION     DAZKJR010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.566"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..>321
                     /locus_tag="ACHOWS_005605"
     CDS             204..>321
                     /locus_tag="ACHOWS_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0050987.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQ"
BASE COUNT          107 a           65 c           76 g           73 t
ORIGIN      
        1 gaagaaaaac gacggcacaa caatggcatg tcgtggtcac tggtgcacca ctcccgcaca
       61 ggtgaaaaca gagaaaacaa cacgacatat acaatcaaaa aaaagtgaaa tttcttcact
      121 ttttcactct tttttatcgc gctgacttgg tgactatgat gataaaataa agtgaagttt
      181 tttcactttt tcaaaggtgt tttatgaccg gctgggaact gcgaatctgg cgaaaaagca
      241 tgctctggtc acgcgaaaaa gcggccagag aatttggcgt gacacagaga acatggcacg
      301 cctgggaaaa tgcggaacag g
//
LOCUS       DAZKJR010000564          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZKJR010000564 DAZKJR010000000
VERSION     DAZKJR010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.567"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>321)
                     /locus_tag="ACHOWS_005606"
     CDS             complement(<1..>321)
                     /locus_tag="ACHOWS_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFQ0050988.1"
                     /translation="QNQSVPEVVEQVLRKHGLEGADFEFRLERAYPSREIITQWQETD
                     LQFIQRILSEVGIFWRTEMDDVRGLDTYIFADSQLNYQFDVCLPYSEPSGLFDGAAES
                     VWDV"
BASE COUNT           81 a          102 c           74 g           64 t
ORIGIN      
        1 gtacatccca gaccgattct gcagcaccgt caaaaaggcc cgacggctcg ctgtacggca
       61 gacacacatc aaactggtag ttaagctggc tgtcggcaaa aatgtacgta tccagtccgc
      121 gcacatcatc catctccgtg cgccagaaaa tccccacctc agacagaatg cgctgaatga
      181 actgcaaatc cgtttcctgc cactgggtga taatttcacg cgacgggtaa gcgcgttcca
      241 gtctgaattc aaaatcagcg ccctccagcc cgtgtttgcg cagcacctgc tccaccacct
      301 cagggacgga ctggttctga a
//
LOCUS       DAZKJR010000565          312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZKJR010000565 DAZKJR010000000
VERSION     DAZKJR010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.denovo.569"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..>312
                     /locus_tag="ACHOWS_005607"
     CDS             17..>312
                     /locus_tag="ACHOWS_005607"
                     /inference="COORDINATES: protein motif:HMM:NF017942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFQ0050989.1"
                     /translation="MSAGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRA
                     RNDQHQFDGYVTYKVNDSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMS"
BASE COUNT          104 a           55 c           67 g           86 t
ORIGIN      
        1 gattcagaca caatggatgt cagcaggaag tcagtataac ccttatctga aaatttcgta
       61 tcaggcaacg ccagatctta atctggcctt acgctatcgc tatgactgga aagctttccg
      121 acagacggat ttagatggca acagggcgcg taatgatcag catcagtttg atggttatgt
      181 gacatacaaa gttaatgatt catggttgtt tgcatggcag accacggtat atacgaaggt
      241 taataatttc aaatatggga atcataaaaa aacagcaact gaaaatgcat ttgttctgca
      301 atataaaatg tc
//
LOCUS       DAZKJR010000566        19618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.guided.096, whole genome shotgun sequence.
ACCESSION   DAZKJR010000566 DAZKJR010000000
VERSION     DAZKJR010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.guided.096"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            214..2022
                     /gene="frdA"
                     /locus_tag="ACHOWS_005608"
     CDS             214..2022
                     /gene="frdA"
                     /locus_tag="ACHOWS_005608"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFQ0050990.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2015..2749
                     /gene="frdB"
                     /locus_tag="ACHOWS_005609"
     CDS             2015..2749
                     /gene="frdB"
                     /locus_tag="ACHOWS_005609"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFQ0050991.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2760..3155
                     /gene="frdC"
                     /locus_tag="ACHOWS_005610"
     CDS             2760..3155
                     /gene="frdC"
                     /locus_tag="ACHOWS_005610"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFQ0050992.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3166..3525
                     /gene="frdD"
                     /locus_tag="ACHOWS_005611"
     CDS             3166..3525
                     /gene="frdD"
                     /locus_tag="ACHOWS_005611"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFQ0050993.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3588..4721
                     /locus_tag="ACHOWS_005612"
     CDS             3588..4721
                     /locus_tag="ACHOWS_005612"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFQ0050994.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4810..5343
                     /gene="blc"
                     /locus_tag="ACHOWS_005613"
     CDS             4810..5343
                     /gene="blc"
                     /locus_tag="ACHOWS_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFQ0050995.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5344..5661)
                     /gene="sugE"
                     /locus_tag="ACHOWS_005614"
     CDS             complement(5344..5661)
                     /gene="sugE"
                     /locus_tag="ACHOWS_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFQ0050996.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5836..5982)
                     /gene="ecnB"
                     /locus_tag="ACHOWS_005615"
     CDS             complement(5836..5982)
                     /gene="ecnB"
                     /locus_tag="ACHOWS_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFQ0050997.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6093..6218)
                     /gene="ecnA"
                     /locus_tag="ACHOWS_005616"
     CDS             complement(6093..6218)
                     /gene="ecnA"
                     /locus_tag="ACHOWS_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFQ0050998.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(6270..6836)
                     /gene="efp"
                     /locus_tag="ACHOWS_005617"
     CDS             complement(6270..6836)
                     /gene="efp"
                     /locus_tag="ACHOWS_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0050999.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6878..7906
                     /gene="epmB"
                     /locus_tag="ACHOWS_005618"
     CDS             6878..7906
                     /gene="epmB"
                     /locus_tag="ACHOWS_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFQ0051000.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            8141..9010
                     /locus_tag="ACHOWS_005619"
     CDS             8141..9010
                     /locus_tag="ACHOWS_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFQ0051001.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(9060..9413)
                     /locus_tag="ACHOWS_005620"
     CDS             complement(9060..9413)
                     /locus_tag="ACHOWS_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFQ0051002.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(9551..11197)
                     /gene="groL"
                     /locus_tag="ACHOWS_005621"
     CDS             complement(9551..11197)
                     /gene="groL"
                     /locus_tag="ACHOWS_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0051003.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(11241..11534)
                     /locus_tag="ACHOWS_005622"
     CDS             complement(11241..11534)
                     /locus_tag="ACHOWS_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0051004.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            11810..13066
                     /gene="yjeH"
                     /locus_tag="ACHOWS_005623"
     CDS             11810..13066
                     /gene="yjeH"
                     /locus_tag="ACHOWS_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFQ0051005.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(13082..13558)
                     /locus_tag="ACHOWS_005624"
     CDS             complement(13082..13558)
                     /locus_tag="ACHOWS_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFQ0051006.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            13895..15331
                     /gene="aspA"
                     /locus_tag="ACHOWS_005625"
     CDS             13895..15331
                     /gene="aspA"
                     /locus_tag="ACHOWS_005625"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFQ0051007.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            15450..16751
                     /gene="dcuA"
                     /locus_tag="ACHOWS_005626"
     CDS             15450..16751
                     /gene="dcuA"
                     /locus_tag="ACHOWS_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFQ0051008.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            16867..17205
                     /gene="cutA"
                     /locus_tag="ACHOWS_005627"
     CDS             16867..17205
                     /gene="cutA"
                     /locus_tag="ACHOWS_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFQ0051009.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            17181..18878
                     /gene="dsbD"
                     /locus_tag="ACHOWS_005628"
     CDS             17181..18878
                     /gene="dsbD"
                     /locus_tag="ACHOWS_005628"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFQ0051010.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIANVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            18915..19490
                     /locus_tag="ACHOWS_005629"
     CDS             18915..19490
                     /locus_tag="ACHOWS_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0051011.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         4767 a         5075 c         4940 g         4836 t
ORIGIN      
        1 tctgatggtt tagtaattaa attaatcatc ttcagtgata atttagccct cttgcgcact
       61 aaaaaaatcg atctcgtcaa atttcagact tatccatcag actatactgt tgtacctata
      121 aaggagcagt ggaatagcgt tcgcagaccg taactttcag gcacttaccc tgaagtacgg
      181 ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa cctttcaagc cgatcttgcc
      241 attgtaggcg ccggtggcgc gggattacgt gctgcaattg ctgccgcgca ggcaaatcca
      301 aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc gtagccatac cgttgctgca
      361 gaagggggct ccgccgctgt cgcgcaggat catgacagct tcgaatatca ctttcacgat
      421 acagtagcgg gtggcgactg gttgtgtgag caggatgtcg tggattattt cgtccaccac
      481 tgccccaccg aaatgaccca actggaactg tggggatgcc catggagccg tcgcccggat
      541 ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg agcgcacctg gttcgccgcc
      601 gataagaccg gcttccatat gctgcacacg ctgttccaga cctctctgca attcccgcag
      661 atccagcgtt ttgacgaaca tttcgtactg gatattctgg ttgatgatgg tcatgttcgc
      721 ggcctggtag caatgaacat gatggaaggc acgctggtgc agatccgtgc taacgcggtc
      781 gttatggcta ccggcggtgc gggtcgcgtt tatcgttaca ataccaatgg cggcatcgtt
      841 accggtgacg gtatgggtat ggcgctaagc cacggcgttc cgctgcgtga catggaattc
      901 gttcagtatc acccaaccgg tctgccaggt tccggtatcc tgatgaccga aggctgccgc
      961 ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt atctgcaaga ttacggcatg
     1021 ggcccggaaa ctccgctggg cgagccgaaa aacaaatata tggaactggg tccacgcgac
     1081 aaagtttctc aggccttctg gcacgaatgg cgtaaaggca acactatctc cacgccgcgt
     1141 ggcgatgtgg tttatctcga cttgcgtcac ctcggcgaga aaaaactgca tgaacgtctg
     1201 ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg atccggttaa agaaccgatt
     1261 ccggtacgtc cgaccgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa
     1321 acccgcatta aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgca
     1381 aaccgtctgg gttctaactc cctggcggaa ctggtggtct tcggccgtct ggccggtgaa
     1441 caagcgacag agcgtgcagc aactgccggt aatggcaacg aagcggtaat tgaagcgcag
     1501 gcagctggcg ttgaacaacg tttgaaagat ctggttaacc aggatggcgg cgaaaactgg
     1561 gcgaagatcc gcgacgaaat gggcctggct atggaagaag gctgcggtat ctaccgtacg
     1621 ccggaactga tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaaacgc
     1681 gtgcgcatca ccgacacttc cagcgtgttc aacaccgacc tgctctacac cattgaactg
     1741 ggccacggtc tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc
     1801 cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt caacttcctc
     1861 aaacacaccc tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg
     1921 aagattacta cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat
     1981 aaggcggaag cagccaataa gaaggagaag gcgaatggct gagatgaaaa acctgaaaat
     2041 tgaggtggtg cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt
     2101 gccttatgac gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc
     2161 accggacctg agctaccgct ggtcctgccg tatggcgatt tgtggttcct gcggcatgat
     2221 ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat
     2281 gaaggttgaa gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccca
     2341 cttcatcgaa agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga
     2401 tcagggtact aacatccaga ccccggcgca gatggccaag tatcaccagt tctccggttg
     2461 catcaactgt ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat
     2521 cggtccggct gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa
     2581 gaaggagcgt atggcgcagt tgaacagcca gaacggcgta tggagctgta ctttcgtggg
     2641 ctactgctcc gaagtctgcc cgaaacacgt cgatccggct gcggccattc agcagggcaa
     2701 agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca
     2761 tgacgactaa acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc
     2821 cgttttatcg cttctacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca
     2881 ttgaactgat tttcgggctg tttgccctga aaaatggccc ggaagcctgg gcgggattcg
     2941 tcgacttttt acaaaacccg gttatcgtga tcattaacct gatcactctg gcggcagccc
     3001 tgctgcacac caaaacctgg tttgagctgg caccaaaagc ggccaatatc attgtaaaag
     3061 acgaaaaaat gggaccagag ccaattatca aaagcctctg ggcggtaact gtggttgcca
     3121 ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat
     3181 ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc
     3241 gcaattattg cgccggtgat gatcctgctg gttggtattc tgctgccact ggggctgttt
     3301 ccgggtgacg cactgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc
     3361 gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac
     3421 gcgatgcacg atctgaaaat ccacgtacct gcgggcaaat gggttttcta cggtctggct
     3481 gctatcctga cagttgtcac gctgattggt gtcgttacaa tctaacgcat cgccaatgta
     3541 aatccggccc gcctatggcg ggccgttttg tatggaaacc agaccctatg ttcaaaatga
     3601 cgctctgcgc cttattaatt accgcctctt gctccacatt tgctgcccct caacaaatca
     3661 acgatattgt gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatag
     3721 cggtggcggt aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg
     3781 ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat
     3841 ttacgggcgt gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatcccg
     3901 cgacaaaata ctggcctgaa cttaccgcta aacaatggaa tgggatcaca ctattacatc
     3961 tcgcgaccta caccgctggc ggcctgccat tgcaggtgcc ggatgacgtt aaatcctcaa
     4021 gcgacttgct gcgcttctat caaaactggc aacctgcatg ggctccagga acacaacgtc
     4081 tgtatgccaa cgccagtatt gggttgttcg gtgcactggc ggtgaaacca tccggtttga
     4141 gctttgaaca ggcgatgcaa actcgtgttt tccagccact caaactcact catacgtgga
     4201 ttaatgtgcc gtccgcagaa gaaaagaatt acgcctgggg atatcgcgaa ggtaaggcag
     4261 tgcatgtttc gccaggggcg ttagatgctg aggcttatgg tgtgaagtcg accattgaag
     4321 atatggcccg ctgggtgcaa agcaatttaa aaccccttga tatcactgag aaaacgcttc
     4381 aacaagggat acaactggca caatctcgct actggcaaac cggcgatatg tatcagggcc
     4441 tgggctggga aatgctggac tggccggtca atcctgacat catcattaac ggcagtgaca
     4501 ataaaattgc actggcagca cgccccgtaa aagcgattac gcccccaact cctgcagtac
     4561 gcgcatcatg ggtacataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta
     4621 ttccagaaaa agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga
     4681 gagtcgccgc cgcctgtcag attctcaacg ctctacagta aaatttcatc gggtccgaat
     4741 ttttggacct tttctccgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag
     4801 gaaacattta tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt
     4861 gcctgcagtt ctcctacgcc gccccgtggc gtgaccgtag tgaataattt cgatgctaaa
     4921 cgctatctcg gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg
     4981 gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt cattaataaa
     5041 ggctataacc ctgacagaga aatgtggcag cagagtgaag ggaaagcgta ctttaccggc
     5101 gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggttataac
     5161 gttattgcac tcgatcggga ataccgccat gcgctggttt gcggtccgga ccgcgactac
     5221 ctgtggatac tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca
     5281 gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt gggtacagca gcccggtagt
     5341 tagttagtga gtgctgagtt tcagaccaat aatccccaat acgattaacg ccagactcgc
     5401 caggcgcatc gggttagcgg actcgccgag cagcacaatg ccggtgatgg ccgcgccgac
     5461 tgcgccaata cccgtccaca cggcataagc cgtccctact ggtaacgatt tcatcgccca
     5521 ggcaagtagc gccatactga caatcatcgc cgtcacagta ataacacttg gcgtcaaacg
     5581 actaaagccg tgggtatatt tcaggccaac ggcccatacc acttccagca ggccagcaat
     5641 aactaagata atccaggaca tatcaggctc cggagaattg gggccgtccc cggtgaaaag
     5701 aggcgtttgc aggtcgtcct gcaaagctga tggttgaacg gatattttgc caggtggcag
     5761 gggaatttca actattacct ttgtgactat cgcaaaaaaa caaacggcac gacacagctg
     5821 tcgtaccgtt attgcttact gctgcgcttt cgttgctgcg ccagaaatcg cgttaccgcc
     5881 atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcaa gcagttaata ctgttgaaag
     5941 caccagaaca gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt ccttttaggt
     6001 aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca aaaatgatgg
     6061 aaatctgatg atttttcgga ggagaagaaa aattagctgg cagcgcggga gatggagttg
     6121 ccgagatgtt taatgtcttc accaaaaccg cgagcggtgt tacagcccgt gagcagcgca
     6181 ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa taagtgatgg
     6241 tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta ttcaccagag
     6301 cgggtatcca ctttgatgac ttcgccgatc tgtacgaaca gcggaacttt aaccacagcg
     6361 ccagtagaca gggtagctgg tttgccgcca gtacctgcgg tatcaccttt caggcccgga
     6421 tcggtatcaa cgatttccag ctcaacgaag ttcggcggag taacggagat cggctgacca
     6481 ttccacagag ttacgataca ctctgcctga tccagcaacc atttggcgtt gtcgccaatt
     6541 gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca
     6601 ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcaccttc agcggaatca
     6661 gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag tttaacgcga
     6721 gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc gtaaggttcg
     6781 ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt tgccataagg
     6841 ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg taaccctaaa
     6901 taccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg atcctgatga
     6961 acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccgggc gcagcgccaa
     7021 aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga aaggcaatcc
     7081 gaacgatccg cttttgcgtc aggtacttac ctcgcaagat gagtttgttg tcgcgcccgg
     7141 attctccacc gacccgctgg aagaacagca cagcgtagtg ccaggtttgt tgcataaata
     7201 ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc gctattgctt
     7261 ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag cgtaactggc agactgcact
     7321 tgagtatgtt gctgcgcatc cggaactgga cgagatgatt ttctccggcg gcgatccgct
     7381 gatggcgaaa gatcacgagc tggactggtt gctcacacaa ctggaagcca tcccgcatat
     7441 caaacgtctg cggattcaca gccgtctgcc gattgtgatc ccggcacgca tcacagatgc
     7501 gctggttgaa cgcttttctc attctacgct gcaaatcttg ctggtgaatc acattaacca
     7561 tgccaatgag gtagatgaaa cattccgtca ggcgatggct aagttgcgcc gggtaggcgt
     7621 tactttgctc aaccagagcg ttctgttacg tggtgtgaac gataacgcgc aaacgctggc
     7681 aaacctgagt aatgcgttgt tcgatgcggg cgtgatgccg tattacctgc atgtgctcga
     7741 taaagtacag ggcgcggcgc attttatggt gagtgatgac gaagcacggc agattatgcg
     7801 tgagttgctg acactggtgt cgggatatct ggtgcctaaa ctggcgcgag aaattggcgg
     7861 cgaacccagc aaaacgccgc tggatctcca gctacgccag cagtaactga ttaaacaggg
     7921 aagtttaatc tttgcacgtt gcataacctt taattattca tatacattgc tgttattact
     7981 ttttatataa ccaacaccaa taattaacct ttcttttctg tttatataga gtgagcacgg
     8041 ctatttatgt tattttaaca actattacta ccaagcccat ttatctggct tataaattaa
     8101 aattaattat ttaattgaga aatggttagg gagaacctac atggccataa gcatcaaggg
     8161 tgttaatacc ggtgttattc gcaagagcaa taatttcatt gcactggcgc tgaaaattaa
     8221 agagcctcgc aataaagaat cgttgttctt tctgtctgtc atggaactgc gcgaccttct
     8281 gattgcgctg gaaagccgtc tgcatcaaaa gcataaactg gatgctgctg cacgccttca
     8341 gtatgagcaa gctcgcgata aagtcattaa gaaaatggta gaaaatatcc cagaaattct
     8401 ggttgatgag cttaaaaatg ccgatattaa tcgtcgggtt aacacgctgg aacttaccga
     8461 taatcagggc gaaaatctta ccttcgtatt aacactacat gacggtagca aatgtgagtt
     8521 agtcatcaat gaattacaga ttgaaatgct ggcgcgagca atcattcatg caattaataa
     8581 tgcggaaatg cgtgaactgg cattacgtat tacttcactg ttagatttcc tgccgttata
     8641 cgacgttgac tgccaggaca atggcaatct ggaatatgac acctattccc aaccggaatg
     8701 gaaacataac ttgttcaacc attatctggc ggtgctttac cgctttaaag atgaaagtgg
     8761 caaagaacaa tttagcggcg cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaagt
     8821 ggaggcgatc actcgccgaa tgcttgattt cagcccccga ctgaaaaaac tcgcgggtgt
     8881 gccatgtcag gtctatgttc gcaccgtagc ggcaaataac gcccaaccgc taacccagga
     8941 tcaatgctta cgcgcgctgc atcacttacg tgttcaatcc accagcaaaa ctgcaccaca
     9001 ggcgaaataa tctcatctgt tttactacga ggctggaagt atccagcctc tggatcacat
     9061 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     9121 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     9181 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     9241 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     9301 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     9361 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     9421 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     9481 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgagg
     9541 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     9601 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     9661 ggccagccac agaagctgcg tactgcagag cagaacgagt tactttggtt ggatccagga
     9721 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     9781 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aatacgatct
     9841 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     9901 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     9961 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
    10021 ttttctcttt catttcaact tcggtagcag cacccacttt gataactgca acgccgcctg
    10081 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
    10141 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcaa
    10201 tgatggtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
    10261 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
    10321 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
    10381 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc cagcgcttcg ccttctacat
    10441 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
    10501 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
    10561 ctgcgccagt ttccggcttg ttgataaagt aaggagacag gtagccacgg tcgaactgca
    10621 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
    10681 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
    10741 tagcggagat ggtaccaacc tgagcaatcg ctttagagtc agagcatggt acggacagcg
    10801 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
    10861 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
    10921 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
    10981 tctgcgcacc catgttttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
    11041 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggcctttcg
    11101 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
    11161 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
    11221 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
    11281 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
    11341 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
    11401 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
    11461 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
    11521 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
    11581 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
    11641 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
    11701 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
    11761 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga catcaaccca tgagtggact
    11821 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
    11881 tggcgtgttt gccgttcctg cgttagctgc actggtagcg ggcaataaca gcctgtgggc
    11941 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
    12001 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
    12061 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
    12121 acaaattgcc gccgggttcg gccaggcaat gtttggctgg catagctggc aactgttgtt
    12181 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actcgcggtg ccagttccag
    12241 tgcaaatcta caaaccgtta ttgccggact tatcgtcgcg ctgattgtcg ctatctggtg
    12301 ggcgggcgat atcaaacctg cgaatatccc cttcccggca cctggtaata tcgaacttac
    12361 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aggcatttgc
    12421 ccatctcgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
    12481 tctgctgctg gcaggattag tctactgggg atgtacggta gtcgtcttac acttcgacgc
    12541 ctatggtgaa aaaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg
    12601 tgtaggagcg ttatggattg cctgcgtgat tggctatctg gcctgctttg ccagtctcaa
    12661 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
    12721 ctacctgtca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgctcgg
    12781 ctgctgcgtg gtgagcactt tggtgattca tgctttagag atcaatctgg acgctcttat
    12841 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
    12901 attattgcaa ggacgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
    12961 actgacaatg gtcggctgga aaagtctcta tgcgctgatc atgctggcgg ggttatggct
    13021 gtttctgcca aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac
    13081 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctggt
    13141 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
    13201 gagaaaagcg caaatgcggc atcaacttca ctgtcagatg cttttgcacc ggcggcaaca
    13261 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
    13321 tgatcagcga aacactttta atcatctccg ccgctgggtt ttcacccgcc gccatttttt
    13381 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
    13441 acgtgaatat aaccagcacg agagtcagca atacccccaa tacatgggca acctgaataa
    13501 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
    13561 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt
    13621 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
    13681 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
    13741 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
    13801 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
    13861 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
    13921 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
    13981 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
    14041 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
    14101 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
    14161 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
    14221 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
    14281 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
    14341 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
    14401 actgcgtgaa ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg
    14461 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
    14521 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
    14581 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
    14641 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
    14701 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
    14761 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tcaggcccac gtgccggcct
    14821 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
    14881 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
    14941 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggagc cggtcattgg
    15001 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
    15061 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
    15121 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
    15181 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
    15241 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
    15301 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
    15361 acgtcagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atctaatatc
    15421 aacttgttaa aaaaacaagg aaggctaata tgctagttgt agaactcatc atagttttgc
    15481 tggcgatctt cttgggcgcc agattggggg gaataggtat tggttttgca ggcggattgg
    15541 gggtgctggt tcttgccgct attggcgtta aacccggtaa catcccgttc gatgtcattt
    15601 ccattatcat ggcggttatc gccgctattt ctgccatgca ggttgctggc ggtctggact
    15661 atctggttca tcagacagaa aagctgctgc gccgtaaccc gaaatacatc acgatcctcg
    15721 caccgatcgt gacctatttc ctgacaatct ttgctggtac tggcaacatc tctctggcga
    15781 cactgccagt tatcgctgaa gttgcgaagg aacaaggcgt taaaccttgc cgtccgctgt
    15841 ctactgcagt ggtatccgcg cagattgcga tcaccgcatc gccaatctca gcggcagtgg
    15901 tttacatgtc ttccgtaatg gaaggtcatg gcatcagcta cctccatctg ctctccgtgg
    15961 tcatcccgtc caccctgctg gcggttctgg tgatgtcctt cctggtcact atgctgttca
    16021 actccaaact ctctgacgat ccgatttatc gcaagcgtct ggaagaaggc ctggttgaac
    16081 tgcgcggtga aaagcagatt gaaatcaaat ccggtgcaaa aacgtccgtc tggctgttcc
    16141 tgctgggcgt agttggcgtt gttatctatg caatcatcaa cagcccaagc atgggtctgg
    16201 ttgaaaaacc gctgatgaac accaccaacg caatcctgat catcatgctc agcgttgcaa
    16261 ctctgaccac cgttatctgt aaagtcgata ccgacaacat cctcaactcc agcaccttca
    16321 aagcaggtat gagcgcctgt atttgtatcc tgggtgttgc gtggctgggc gatactttcg
    16381 tttccaacaa catcgactgg atcaaagata ccgctggtga agtgattcag ggtcatccgt
    16441 ggctgctggc cgtcatcttc ttctttgctt ctgctctgct gtactctcag gctgcaaccg
    16501 caaaagcact gatgccgatg gctctggcac tgaacgtttc tccgctgacc gctgttgctt
    16561 ctttcgctgc ggtgtctggt ctgttcattc tgccgaccta cccgacgctg gttgctgcgg
    16621 tacagatgga tgacacgggt actacccgta tcggtaaatt cgtcttcaac catccgttct
    16681 tcatcccggg tactctgggt gttgccctgg ccgtttgctt cggcttcgtg ctgggtagct
    16741 tcatgctgta atgatcaatc gcggggcgtt cacgccccgc tttctttccc gccgactaac
    16801 atccttcccc cgtccgttgt atagtgacct ctctcttgcg gttccatctg ttcttgcgag
    16861 gtgtttatgc ttgatgaaaa aagttcgaat accgcgtctg tcgtggtgct atgtaccgca
    16921 ccggatgaag cgacagccca ggatttagcc gccaaagtgc tggcggaaaa actggcggcc
    16981 tgcgcgacct tgatccccgg cgctacttct ctctattact gggaaggtaa gctggagcaa
    17041 gaatacgaag tgcagatgat tttaaaaact accgtatctc accagcaggc actgctggaa
    17101 tgcctgaagt ctcatcatcc atatcaaacc ccggaacttc tggttttacc tgttacacac
    17161 ggagacacag attacctctc atggctcaac gcatctttac gctgatcctg ctactttgca
    17221 gcacttccgt ttttgccgga ttattcgacg cgccgggacg ttcacaattt gtccccgcgg
    17281 atcaagcctt tgcttttgat tttcagcaaa accaacatga cctgaatctg acctggcaga
    17341 tcaaagacgg ttattacctc taccgtaaac agatccgcat tacgccggaa cacgcgaaaa
    17401 ttgccaacgt gcagctgcca caaggcgtct ggcatgaaga tgagttttac ggcaaaagcg
    17461 agatttaccg cgatcggctg acgcttcccg taaccatcaa ccaggcgagt gcgggagcga
    17521 cgttaactgt cacctaccag ggctgtgctg atgccggttt ctgttatccg ccagaaacca
    17581 aaaccgttcc gttaagcgaa gtggtcgcca acaacgcagc gtcacagcct gtgtctgttc
    17641 cgcagcaaga gcagcccacc gcgcaattgc ccttttccgc gctctgggcg ttgttgatcg
    17701 gtattggtat cgcctttacg ccatgcgtgc tgccaatgta cccactgatt tctggtatcg
    17761 tgctgggtgg taaacagcgg ctctccactg ccagagcatt gttgctgacc tttatttatg
    17821 tgcaggggat ggcactgact tacacggcgc tgggtctggt agttgccgcc gcagggttac
    17881 agttccaggc ggcgctacag cacccatacg tgctcattgg cctcgccatc gtctttacct
    17941 tgctggcgat gtcaatgttt ggtttgttta ccctgcaact cccctcttcg ctgcaaacgc
    18001 gcctcacgct gatgagcaat cgccaacagg gcggctcacc tggcggtgtg tttgttatgg
    18061 gagcgattgc cggactgatc tgttcaccat gcaccaccgc accgcttagc gcgattctgt
    18121 tgtatatcgc ccaaagcggg aacatgtggc tggggggcgg cacgctttat ctctatgcgt
    18181 tgggcatggg cctgccgctg atgctaataa ccgtctttgg taaccgcctg ctgccgaaaa
    18241 gcggcccgtg gatggaacaa gtcaaaaccg cgtttggttt tgtgatcctc gcactgccgg
    18301 tcttcctgct ggagcgagtg attggcgata tatggggatt acgcttgtgg tcggcgcttg
    18361 gtgtcgcatt ctttggctgg gcctttatca ccagcctaca ggccaaacgc ggctggatgc
    18421 gcgtggtgca aataatcctg ctggcagcgg cattggttag cgtgcgccca cttcaggatt
    18481 gggcatttgg tgcaacacat accgcgcaaa ctcagacgca tctcaacttt acacaaatca
    18541 aaacggtaga tgagttaaat caggcgctcg ttgaagccaa aggcaaacca gtgatgttag
    18601 atctctatgc cgactggtgc gtcgcctgta aagagtttga gaaatacacc ttcagcgacc
    18661 cgcaggtgca aaaagcgtta gcagacacgg tcttacttca ggctaacgta actgccaacg
    18721 acgcacaaga tgtggcgctg ttaaagcatc ttaatgtcct tggcctaccg acaattctct
    18781 tttttgacgg acaaggccag gagcatccac aagcacgcgt cacgggcttt atggatgctg
    18841 aaaccttcag cgcacatttg cgcgatcgcc aaccgtgaac gacacttgca gtgggaaaga
    18901 cggaggaaaa tagcgtgcaa cgtgaagatg tactgggaga agccctgaaa ttattagaat
    18961 tacaagggat tgccaacacc acgcttgaga tggttgctga acgtgtggat tacccactgg
    19021 acgagctacg ccgcttctgg ccagacaaag aggcaatcct ctacgatgcg ctgcgttatc
    19081 ttagccaaca gatagatgcc tggcgtcgtc agctgatgct ggacgaaacg caaaccgccg
    19141 aacaaaagct gctggcacgt tatcaggcgt tatcggagtg cgttaaaaac aaccgctatc
    19201 cgggctgtct atttatcgct gcctgtacgt tttatcccga tcctggccac cctattcatc
    19261 aactggccga tcagcaaaaa agcgcggcct acgatttcac ccacgaactg ttaaccacac
    19321 tggaagttga cgatccggcg atggtagcaa agcagatgga actggtgctg gaaggctgtt
    19381 taagccgaat gctggtgaat cgtagccagg cggatgtcga caccgcacat cggctggcgg
    19441 aagatatcct gcgcttcgcc cgctgccgcc agggtggtgc tttgacctga cagaaacaca
    19501 gaaaagaagc gatttgccgc aatcttaagc agttgaatcg cttttactga aattaggttg
    19561 acgagatgtg cagattacgg tttaatgcgc cccgttgccc ggatagctca gtcggtag
//
LOCUS       DAZKJR010000567         2981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.guided.272, whole genome shotgun sequence.
ACCESSION   DAZKJR010000567 DAZKJR010000000
VERSION     DAZKJR010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.guided.272"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..326
                     /locus_tag="ACHOWS_005630"
                     /pseudo
     CDS             120..326
                     /locus_tag="ACHOWS_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            322..674
                     /gene="prfH"
                     /locus_tag="ACHOWS_005631"
                     /pseudo
     CDS             322..674
                     /gene="prfH"
                     /locus_tag="ACHOWS_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHOWS_005632"
     CDS             complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHOWS_005632"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFQ0051012.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2449..2907
                     /gene="gpt"
                     /locus_tag="ACHOWS_005633"
     CDS             2449..2907
                     /gene="gpt"
                     /locus_tag="ACHOWS_005633"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0051013.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
BASE COUNT          728 a          740 c          773 g          740 t
ORIGIN      
        1 tgcaggaagg cgacaagcga gtgaatcccc aggagcttac taaagtaagt gactggggtg
       61 aacgaacgcg gtcgcagtac atgcaacttg aagtatgacg agtataaatg gaatgataca
      121 tgggcaaata tattcgtccc ttatccgatg cggtatttac catcgcatct gatgacctgt
      181 ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc aacatgcagc
      241 gcgttgtggg gatgccagat ttacatcccg gacgcggcta cccgattggc gcagcgttct
      301 tctctgttgg tcgtttttac ccggcaagac gtcgcggtaa cggtgctgga aacagaaacg
      361 ggccgctact ctgacacgct gcgttcggcg ctgatttctc tggatggcga taatgcatgg
      421 gcattaagcg aaagttggtg cggcactatt cagtgtattt gtccgagtcc gtatcggcct
      481 catcatgggc gcaaaaactg gtttctgggc atgctaacaa gcggctggcg cgattgctga
      541 ttgcctggaa gctggaacaa cagcaacagg aaaatagcgc ggcgctgaaa tcgcagcggc
      601 gaatgttcca tcaccagatt gaacgtggca acccgcgacg aacgttcaca gggatggctt
      661 ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca
      721 aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagtagt gtccagtaat
      781 gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg atggttggcc
      841 cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg
      901 cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca
      961 gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct ttcgcttcgg
     1021 ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag tctttaccgc
     1081 tgtcgatcag agaacggatc aggcagtgaa tttctacgtt attgtcagtc atggtcacca
     1141 caccgacgtt cagggaggtt tcgaccacac cttttgccac gtcggagttg cggatcacac
     1201 cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag
     1261 ctttatcgtt cgcaacagag tccagcaaca aggccagatt tttctctttc tctgccagct
     1321 cgtttttcag gatctcctga taggtattca ccagagattt caaagcgtcg actttatcag
     1381 ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt
     1441 tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca
     1501 gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg cctttcaggc
     1561 cttttaaggt taacttgaag gtttcaaagc cagctggaac cgcttcacga tctaaatgca
     1621 ggttggaggt gaagtcgata cctcccgcac aacccatgta gatttcacct tcttcttcgg
     1681 agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagcca aaagcaccgt
     1741 ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt
     1801 cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg
     1861 tgccgcgtgc tttaacccat tcgccatcaa tataaggctg gataggatct ttggtgaagt
     1921 catgcacggt gtcgttattt ttttgcggca ccatatcgag gtgggcctgc aagacgaccg
     1981 gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat
     2041 cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct
     2101 cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct
     2161 gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca
     2221 gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata
     2281 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     2341 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
     2401 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
     2461 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
     2521 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg
     2581 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     2641 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     2701 atcgttattg atgacctggt agataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     2761 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgat
     2821 tatgttgttg atatcccgca aaatacctgg atcgaacagc cgtgggatat gggcgtcgta
     2881 ttcgtgccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     2941 ctttttaact tcaggcgggt tacaatagtt tccagtaagt a
//
LOCUS       DAZKJR010000568         1450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1910T71129
            SAMN43267101-rid23081563.guided.358, whole genome shotgun sequence.
ACCESSION   DAZKJR010000568 DAZKJR010000000
VERSION     DAZKJR010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267101
            Sequence Read Archive: SRR30307141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350459.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350459.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:11:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,635
            CDSs (total)                      :: 5,537
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 98
            rRNAs                             :: 4, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 43 of 178
            Pseudo Genes (multiple problems)  :: 50 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267101-rid23081563.guided.358"
                     /serotype="O146:H28"
                     /isolate="1910T71129"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2019-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..1076
                     /gene="stxA2b"
                     /locus_tag="ACHOWS_005634"
     CDS             117..1076
                     /gene="stxA2b"
                     /locus_tag="ACHOWS_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFQ0051014.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1089..1352
                     /gene="stxB2b"
                     /locus_tag="ACHOWS_005635"
     CDS             1089..1352
                     /gene="stxB2b"
                     /locus_tag="ACHOWS_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFQ0051015.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          396 a          292 c          350 g          412 t
ORIGIN      
        1 ggttcgaatc cagtacaacg cgccatactt attttttctg gctcgctttt gcgggccttt
       61 tttatatctg cgccgggtct ggtgctgatt acttcagcca aaaggaatac ctgtatatga
      121 agtgtatatt gttaaaatgg gtactgtgcc tgttactggg cttttcttcg gtatcctatt
      181 cccgggaatt tacgatagac ttttcgaccc aacaaagtta tgtctcttcg ttaaatagta
      241 tacggacaga aatatcgacc ccacttgaac atatatctca ggggaccaca tcggtgtctg
      301 ttattaacca caccccaccg ggcagttatt ttgctgtgga tatacgaggg cttgatgtct
      361 atcaggcgcg ttttgaccat cttcgtctga ttattgagca aaataattta tatgtggccg
      421 gattcgttaa tacggcaaca aatactttct acaggttttc agattttgca catatatcag
      481 tgcccggtgt gacaactgtt tccatgacaa cggacagcag ttataccact ctgcagcgtg
      541 tcgcagcgct ggaacgttcc ggaatgcaaa tcagtcgtca ctcactggtt tcatcatatc
      601 tggcgttaat ggagtttagt ggaaatgcca tgaccagaga tgcatccaga gcagttctgc
      661 gttttgtcac tgtcacagca gaagccttac ggttcaggca aatacagaga gaatttcgtc
      721 tggcactgtc tgaaactgct cctgtttata cgatgacacc ggaagaagtg gacctcacac
      781 tgaactgggg gagaatcagc aatgtgcttc cggagtttcg gggagagggt ggtgtcagag
      841 tggggcgaat atcctttaat aatatatcag cgatactggg cacagtggcg gttatactga
      901 attgccatca tcagggggcg cgttccgttc gcgccgtgaa tgaagagata caaccagaat
      961 gtcagataac tggcgacagg ccagttataa ggataaacaa tacgttatgg gaaagtaata
     1021 ccgcagctgc ttttctgaat cgcagggctc actctttaaa tacatccgga gaataacagg
     1081 agttaaatat gaagaagata tttgtagcgg ctttatttgc ttttgtttct gttaatgcaa
     1141 tggcagctga ttgtgcaaaa ggtaaaattg agttctctaa gtataatgag aatgatacat
     1201 tcacagtaaa agtggccggg aaagagtact ggactaaccg ctggaatctg caaccgctac
     1261 tgcaaagcgc acagttaaca ggaatgacgg taacaatcaa atcaaatacc tgtgcgtcag
     1321 gttcaggatt tgctgaagtg cagtttaatt aatatcagaa gcattgctgg tttcgtggtg
     1381 tgcagcaatg tagttacagt gtaatcaatg tcacaattca gtcagttgaa ggttgtctgc
     1441 ccgactgaga
//
